LOCUS       DATGJW010000001         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_315, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000001 DATGJW010000000
VERSION     DATGJW010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_315"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            234..1388
                     /locus_tag="VKU03_00005"
     CDS             234..1388
                     /locus_tag="VKU03_00005"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HLJ69668.1"
                     /translation="MKRPSYYEFFCGGGMVRAGLGAGWECLLANDIDAKKARAYAANW
                     GTDRLRVGDIHDLSADDAPSRADLAWASFPCQDLSLAGPGGGLKGERSGAFWGFHALM
                     EGLVRAGRAPKIIALENVVGALTSNRGADFVAICRAVQELGYRFGALTIDAAHFTPQS
                     RPRLFIVAMQRTLSVPEGFARDQPSDDFASRALRQAQALLPADLAADWLWWALPAPPK
                     RNVALADLLETSPKDALWRSREETEALLAIFSQGSRAKFEEALRRKGRVVAAVYRRMR
                     PDGEGGRRMRAEARFDGLAGCLRTPRGGSSRQFVLVAEGGVVRSRLLSAREAARLMGL
                     PEEYQLPARYNEAYHLVGDGVAAPVVRFLAEHILESLARSDARPVLEAAE"
     gene            1430..1834
                     /locus_tag="VKU03_00010"
     CDS             1430..1834
                     /locus_tag="VKU03_00010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="very short patch repair endonuclease"
                     /protein_id="HLJ69669.1"
                     /translation="MDRSAVMRAVKSQDTGPERALRKMLRAMAPGYRLHRADLPGKPD
                     VVYASRRLAIFMHGCFWHGHDCKRGARPPKNNADYWRGKIARNRARDEKNVIALEALG
                     WRALTIFECELKDAPGLEARLNEALAGSRDGT"
     gene            complement(1835..>2105)
                     /locus_tag="VKU03_00015"
     CDS             complement(1835..>2105)
                     /locus_tag="VKU03_00015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490918.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FMN reductase"
                     /protein_id="HLJ69670.1"
                     /translation="DWLVGSTEFPGKPVMLINASPRAFHAQASLREILATMSAQLVTE
                     AFVTVKAPSEAKNIDVLLAEAGIVEALRSGVERFAAAIRAQHPKR"
BASE COUNT          353 a          667 c          685 g          400 t
ORIGIN      
        1 aaacctggct catcgcgcca ttcaccttgc cgccgacact cccgtcaagt cgacaacact
       61 tttgcgcccc tgaaaccccg ttgcgacgaa tgctttacgt caccagcgcc tcggcggcgc
      121 tatggctcca ataaaacgcc gaagatgcgt cggcgatcca cgatcgtcgc ttttgcgaaa
      181 ttgcattcgc gaggcggcgc gctagcgtga cggcggagac gattcgccgc cccatgaaac
      241 ggccgagcta ttatgaattt ttttgcggcg gcggcatggt ccgcgccggc cttggcgcgg
      301 gctgggaatg tctcctcgcc aatgacatcg acgccaagaa ggcgcgcgct tatgcggcga
      361 attggggaac ggacaggctt cgcgtcggcg acattcacga cctcagcgct gacgacgcgc
      421 caagtcgcgc cgatcttgct tgggcgtcct tcccttgtca ggatctttcg ctcgcgggcc
      481 ccggcggcgg attgaaagga gaaagatccg gcgccttttg gggctttcac gcgctgatgg
      541 agggactcgt tcgcgcgggg cgcgcgccca aaatcatcgc gttggaaaat gtcgtcggcg
      601 cgctgacctc caatcgcggc gccgatttcg tcgcgatctg ccgcgccgtg caagaactcg
      661 gctatcgctt cggcgctttg acgatcgatg cggcgcattt tacgccgcag tcgcgtccgc
      721 gacttttcat cgtggcgatg caacgtactc tttccgttcc agaaggcttc gcccgcgatc
      781 agccaagcga tgattttgct tcgcgcgcct tgcggcaagc gcaggcgctt ttgcccgccg
      841 atcttgccgc cgactggttg tggtgggcgc tccccgcgcc gcccaagcgc aatgtcgcgc
      901 ttgccgacct tctcgaaacc tcgccaaaag acgcgctttg gcggagccgc gaggagacgg
      961 aggcgctgct cgcgattttc tcgcaaggct ctcgcgccaa atttgaagag gcgctccgcc
     1021 gcaagggccg cgtggtcgca gcggtctatc ggcgcatgcg gccggacggc gaggggggcc
     1081 gtcgcatgcg agccgaggcg cgtttcgatg ggctcgccgg ctgcctgcgc acgccgcgcg
     1141 gcggctccag ccggcagttt gtgctggtcg ctgaaggcgg cgtcgtacga tcccggctgc
     1201 tctctgctcg cgaggccgcg cgattgatgg gtctgcccga agagtaccaa ttgccggcgc
     1261 gctataacga ggcttaccat ctcgtcggcg acggcgtcgc ggcgcctgtc gtacgtttcc
     1321 tggcggagca tatattggag tctttggccc gaagcgacgc ccgcccggtc ctcgaggcgg
     1381 cggaatgacc agtcgaaaac caacgcaacg ggaaaaacca cgcattgcga tggatcgctc
     1441 cgccgtcatg cgggcggtga agtcgcaaga caccgggccg gagcgggcgc tgcgcaaaat
     1501 gcttcgcgcc atggcgccgg gttatcggct gcatcgcgcc gatcttccgg gcaagccgga
     1561 cgtcgtttac gcttcgcgtc gcctggcgat cttcatgcat ggctgctttt ggcatggcca
     1621 tgattgcaag cgtggcgcgc ggccgccgaa gaacaacgcc gactattggc gcggcaagat
     1681 cgcccgcaat cgcgcacgcg acgaaaagaa cgtcatcgcg ctcgaggcgc tcggctggcg
     1741 ggcgctgacg atctttgaat gtgagttaaa agacgcgcct gggctggagg cgcggctcaa
     1801 cgaagccctt gccggttcac gcgatggtac gtagctatcg cttgggatgc tgagcgcgga
     1861 tggccgctgc gaagcgttcg acgccgcttc gcaaggcctc gacgatgccg gcttcagcga
     1921 gcaggacgtc gatgttcttc gcctcgctcg gcgcttttac cgtgacgaac gcctcggtga
     1981 cgagttgcgc cgacatggtt gcgagaattt cgcgtaagct cgcctgagca tgaaaagcgc
     2041 gcggcgacgc gttgatcagc atgaccggct tccccggaaa ttcggtcgag ccgaccagcc
     2101 agtcg
//
LOCUS       DATGJW010000002         5227 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1141583, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000002 DATGJW010000000
VERSION     DATGJW010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5227)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5227)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5227
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1141583"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VKU03_00020"
     CDS             complement(<1..394)
                     /locus_tag="VKU03_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016843605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1697 domain-containing protein"
                     /protein_id="HLJ69671.1"
                     /translation="MTVFVALLRAVNVGGTGKLPMSELKAICEAAGFARVRTYIASGN
                     VVFESKLTEKETKAALEKRLKAYAGKPVGVLVRTAAEMKAVVKDNPFAKAAPNRTVAF
                     FLDEPPPADALARATGVKNEEMRLGRREI"
     gene            531..1301
                     /locus_tag="VKU03_00025"
     CDS             531..1301
                     /locus_tag="VKU03_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ69672.1"
                     /translation="MPDLSLFDLTGRLALVTGSSDGIGLALASGLGRAGARVALNGRN
                     AAKLDAAASRLIGQGIAVETARFDVADRDAVFQAVDRIERDLGAIDILVNNAGVQRRH
                     PFESFPEETWREIMAINIDGVFHVTQAVGKRMVERKRGRIINICSVTSELGRATIVPY
                     ATTKGALRQMTRGLSAEWAKHNVQINAIAPGYIRTPLNQALMDDEKFSAWVAARTPAG
                     RWADAEELVGAAIFLASDAASYVNGHVLYVDGGLSTSV"
     gene            1476..2852
                     /locus_tag="VKU03_00030"
     CDS             1476..2852
                     /locus_tag="VKU03_00030"
                     /inference="COORDINATES: protein motif:HMM:NF013715.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="divalent metal cation transporter"
                     /protein_id="HLJ69673.1"
                     /translation="MSSGAISDLSTPFAGSAPPLSEEDVARAQDRRKVLETRSAGQRL
                     RLLWLLAGPGVLAMLGENDGPSMVSYATTGATYGIGFFVPFILLTFVMAFAVQEMTIR
                     LGAATHRGHAELIFERFGSFWGYFAMGDLVFGNVLTLITEFIAISAGGLYFGVPAWLS
                     VGLALLLVLSSLAFRRYATWERAAMALAAFNLLFIPAALFAHPSGAALAKAVATWSPL
                     QGGVTIAFVTLVMSNIGATVTPWMIFFQQSAVVDKGLTMADAPQARLETAIGAVLAAI
                     AGIATVVAAAPLFVSKVDVSNFSNGADFATALRPLLGTTGATFFALGMIEAGVVAAMT
                     ISTSSAYAFGETLRRRHSLNLDFAQGRWFYGAAILSTLIAAGIVLIPGAPLLALTLTV
                     NVIATLLMAPALLLLLLLVNDREIMGAHANGWRANLVAGTIVVALVLAGAAYGVVTVF
                     PNLLGGQP"
     gene            2849..3034
                     /locus_tag="VKU03_00035"
     CDS             2849..3034
                     /locus_tag="VKU03_00035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69674.1"
                     /translation="MSQEAELIGALEPDQLTAATERALPPRKLGRGMTALLIALRLYI
                     FVAVAIVGYAFVQALTR"
     gene            3371..4564
                     /locus_tag="VKU03_00040"
     CDS             3371..4564
                     /locus_tag="VKU03_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS110 family transposase"
                     /protein_id="HLJ69675.1"
                     /translation="MLRDHLEEPTAIRTQFGAIFVSLELSQKTWLITSLSPGKGEKMS
                     KHGVEARDVAGLLARFAELQRKAAARTGKTFPIIVIQEAGMDGFSIHRVLRQQAIESH
                     VVDPASIAAPRRRRRAKTDRLDGEALLRALLAFKRGEPRVCSMVVAPSVEEEDQRRLC
                     RERDVLIGERISHVNRIKGLLSAQGIADYLPLARDRRKRLLELRTLDGRPLPAHMKAQ
                     ISRELDRLELVVEQIKAVEKERNRLLLAASPEAGGPAVAAAGSKGPALLAELRGLAAD
                     STTKLWSEGLSRSFANRRQVAAYAGLAPTPWRSGSLVHEQGVSKAGNPRLRTTMVQIA
                     WLWLQHQPQSALARWYFAKVGERPTKARKKATIVALARKLLVALWKYATFGEAIAGAA
                     MKRAA"
BASE COUNT          855 a         1692 c         1716 g          964 t
ORIGIN      
        1 agatttcccg ccggccgagc cgcatttctt cgttcttgac gccggtagcg cgagcgagcg
       61 cgtcggcggg cggcggctcg tcgagaaaga acgccaccgt ccggtttggc gccgccttgg
      121 cgaagggatt gtccttgacc acggctttca tttcagccgc ggtgcggacg agaacgccga
      181 ccggcttgcc ggcataggct ttcagacgct tttcgagcgc cgccttcgtc tctttctcgg
      241 tcagcttgct ttcgaatacg acattgccgc tggcgatgta ggtgcggact ctggcgaagc
      301 ccgccgcctc gcatatcgct ttcaactcgc tcatcggcag cttgccggtt ccgccgacat
      361 tgacggcgcg caaaagggcg acgaagacgg tcatcggaac tccaggacag agagggcggg
      421 cgcgcgatgc gccgtcgcgc ggctctcgcg gagatggggc aattcgcgcg caggcgcaag
      481 gccgggcgca tgctatgaga gccggccgtc gctttcacaa aagaactgcc atgcccgatc
      541 tcagcctgtt cgatttgacc ggccgcctcg ccctcgtcac cggctcgtcg gatgggatcg
      601 gcttggcttt ggcgagcggc cttggccggg ccggcgcgcg cgtcgcgctc aacggacgaa
      661 acgctgcgaa actcgacgcc gcggcctcgc gcttgatcgg gcaggggatc gcggtcgaga
      721 cggcgcggtt cgacgtcgcc gatcgcgacg ctgtttttca agccgtcgat cgcatcgagc
      781 gcgatctcgg cgcgatcgac attctggtca acaatgccgg cgtgcagcgc cgccatcctt
      841 tcgagagctt tccggaagag acgtggcgcg agatcatggc gatcaatata gacggcgtct
      901 tccatgtgac tcaggccgtc ggcaagcgca tggtcgagcg caaacgcggc cgcatcatca
      961 atatctgctc ggtgacgagc gagcttggcc gcgccaccat cgtcccctat gcgacgacga
     1021 aaggcgcgct gcgccaaatg acgcgcggcc tgtcggcgga atgggccaag cacaatgtgc
     1081 agatcaacgc catcgcgccc ggctatatcc ggaccccgct caatcaagcc cttatggatg
     1141 acgagaaatt ctccgcctgg gtcgcggcgc gaaccccggc cggccgctgg gccgacgccg
     1201 aagaactggt cggcgctgcg atcttcctcg cctccgacgc cgcctcctat gtcaacggcc
     1261 acgtcctcta tgtcgatggc gggctgtcga ccagcgtctg aggaaatgcg gcggcggcgc
     1321 ttccgcttcg ccgcccgacg ctcggcccgc ggcaagcgcg ccctggcgca taaaataaag
     1381 tattgactac attttgacgc ctgacccgct ttggaaagct cctccataaa tccgaagccc
     1441 attgacttga atatagctaa ggctacatta agcgcatgtc cagcggcgcg atctcggatc
     1501 tctcgacgcc ttttgcgggc tcggcgcccc cgctgagcga ggaggatgtc gcgcgggcgc
     1561 aagaccgccg caaagttctc gaaacgcggt cggcgggcca gcgtttgcgc ctgctctggc
     1621 ttctggccgg gccaggcgtg ctcgccatgc tcggcgagaa tgacgggccg agcatggtct
     1681 cctacgccac gaccggcgcg acctatggga tcggcttttt cgttccattc atcctgctca
     1741 ccttcgtgat ggccttcgcc gtgcaggaaa tgacgatccg cctcggcgcg gcgacgcatc
     1801 gcggccatgc cgagctgatt ttcgagcggt tcggctcgtt ctggggctat ttcgcgatgg
     1861 gcgacctggt gttcggcaat gtgctgacgc tgatcaccga gttcatcgcg atcagcgccg
     1921 gcggcctcta tttcggcgtt ccggcttggc tgtcggtcgg cctcgcgctc ctcctcgttc
     1981 tgtcctcgct cgccttccgc cgctatgcga cctgggagcg cgccgccatg gcgctcgccg
     2041 cgttcaatct tctgttcatc ccggcggctc tgttcgccca tccgagcggc gcggcgctcg
     2101 ccaaggcggt cgcgacctgg agtccgcttc aaggcggcgt cacgatcgct ttcgtgacgc
     2161 tggtcatgtc caatatcggc gcgacagtga cgccctggat gattttcttc cagcagagcg
     2221 cggtcgtcga caagggcctg accatggccg acgcgccgca ggcgcggctg gagacggcga
     2281 tcggggcggt tctggcggcg atcgccggga tcgcgaccgt cgtcgccgcg gcgccgcttt
     2341 tcgtcagcaa ggtcgacgtg tcgaatttct ccaacggcgc cgatttcgca accgccttgc
     2401 ggccattgct ggggacgacc ggcgcgacgt tcttcgcgct cggcatgatc gaggcgggcg
     2461 tggtcgccgc gatgaccatc tcgaccagtt cggcctatgc gttcggcgag acgctgcgcc
     2521 gccggcacag cctcaatctc gatttcgcgc aagggcgctg gttctatggc gcggcgatcc
     2581 tgtcgaccct gatcgcggcg ggaattgtgc tgatccccgg cgcgcctctg ctcgctctca
     2641 ccttgaccgt caacgtcatc gcgacgctgc tcatggcgcc ggcgctgctc ctcctgctgc
     2701 tgctcgtcaa cgaccgcgag atcatgggcg ctcacgccaa tggctggcgc gccaatctcg
     2761 tcgccggtac gatcgtcgtg gcgctcgttc ttgccggcgc cgcctatggc gtcgtcaccg
     2821 tgtttccgaa ccttttggga ggccagccat gagccaggaa gccgaactga tcggcgcgct
     2881 ggagccggac caattgaccg cggcgaccga gcgcgcgctg ccgccccgaa agctcggccg
     2941 cggcatgacg gcattgctca tcgcgctgcg cctctacata ttcgtcgccg tcgcgatcgt
     3001 cggctacgcc ttcgttcaag cgctgacgcg ctgagccctt ctccgcgcga agcgcagggc
     3061 gtcccgggaa agtgagaggc gagtcgcatc gtggtcgacg aagtctcgtg aaataacgcg
     3121 atttcgagat ttcctcgttg agtttccggc gccgcgactc agaaaataaa catgcggacc
     3181 tcggggctgc tcacaaactc ttgcgagcag tacggatgca acgatgacgc aaatccccta
     3241 cgccgcgctg ccacgccggg cgatcgcagt cacccggagg gccggagcga agattccatg
     3301 ggggaggtgg atagcggccg tttctgagaa cagactgagg ttgctgatcc actcaccctg
     3361 gaggcgaccg atgttgcgcg atcacctgga agaaccgacc gctatccgca cgcagtttgg
     3421 cgcgattttc gtttctttgg aactcagtca aaagacttgg ctgatcacct cgctctcgcc
     3481 cggcaagggc gagaagatgt cgaagcacgg cgtcgaggcg agagacgtcg ccgggctctt
     3541 ggcgcgcttt gccgaactgc agcgcaaggc ggcggcgcgg acgggcaaga ctttcccgat
     3601 catcgtcatt caagaagccg gaatggatgg gttctcgatt catcgggtcc tgcgccagca
     3661 ggcgatcgag agccatgtgg tcgatccggc ctcgatcgcg gcgccgcgcc ggcgacggcg
     3721 ggctaagacg gacaggctcg acggcgaggc gttgctgcgg gcgctgctgg ctttcaagcg
     3781 cggcgagccg cgggtctgct cgatggtggt cgcgccttcg gtagaagagg aggaccaacg
     3841 ccgattgtgc cgcgagcgcg acgtgctgat cggcgagcgg atcagccacg tcaaccggat
     3901 caaaggcctt ttgtcggcgc aagggatcgc cgattacctg ccgctggcgc gcgatcgccg
     3961 caagcggctt ttggagctgc gcacgctcga cgggcggccg ttgccggcgc atatgaaggc
     4021 gcagatcagc cgcgagctcg accggctcga actggtggtc gagcagatca aggcggtgga
     4081 gaaggagcgc aatcggctct tgcttgcggc ttcgccggag gctggcggac ccgccgtcgc
     4141 ggcggcgggc tcaaagggcc cggcgctgtt ggccgagctt cgcggcctcg ccgccgattc
     4201 gacgacgaag ctgtggtcgg aaggactatc gcgcagcttc gccaatcgtc ggcaggtcgc
     4261 cgcttacgcc ggccttgcgc cgacgccgtg gcggagcggt tcgctcgtcc atgagcaagg
     4321 cgtgtccaaa gcgggcaatc cgcggctgcg tacgacgatg gtgcagatcg cctggctgtg
     4381 gctgcagcat cagccgcaat cggccttggc gcggtggtat tttgcgaagg tcggcgagcg
     4441 cccgaccaag gcgcgcaaga aggcgacgat cgtggcgctg gcgcgcaagc ttctggtcgc
     4501 gctgtggaag tatgcgacct tcggcgaggc catcgcgggc gcggcgatga agcgagccgc
     4561 ttgaaccgga gaccttcgaa ctcctttcaa agcgaaagga cttcaactca aatcgccgtg
     4621 gctcgatcct cgatcagccc cggccgatcc gggcggacga accgttgctc cgcatggctt
     4681 gaaacgccgc agcgtagatt ggcctcgtcc tctcgagctc gcccgcgcaa gcggaatgat
     4741 ggcgccagtc gcttcgaacg acggccgaat gtgagtttga tctggctgac gcccagatcg
     4801 ttcgcaaaac gggctcgacc ttggatcccg aacgttcgaa aggcccggtc atgtcttgaa
     4861 cccgcttgcc tcccgaccca accagcttag cgccgtgctc gcgtcgtcaa ggccgcttcg
     4921 cgccgccttc ggcggcggcc tgacggccgg ccttgacgac gcttgcgcgc ggcgctccgc
     4981 taggtctcag gtcgggacga agggatggtc gtttcggtcg aacaaaggga tgtcccgcca
     5041 aaacgcggtc tttgtccgcg gcccttgaca cgaaacgccc catgtgagcg gaggacccgc
     5101 catagcgccg cacaagcgga tatgatcttg cacgtagctt gccggcgcta tggcgggttg
     5161 acggcgatcg gccggcgtgg cgccgcggcg ctgaaagatt acgtccaaga cgcgccataa
     5221 cctcaga
//
LOCUS       DATGJW010000003         6745 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1245381, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000003 DATGJW010000000
VERSION     DATGJW010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6745)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6745)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6745
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1245381"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(88..1092)
                     /locus_tag="VKU03_00045"
     CDS             complement(88..1092)
                     /locus_tag="VKU03_00045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ69676.1"
                     /translation="MRDGRGVAFGSAAAQRRALFLAVAVALAGCAGPAPVRQAAAPPT
                     SSGFTQAGGVNVAYAEFQNSAFPYHGAIPDEGRPFLDVNANGRLGHSSPRGGVYWEDE
                     TYNDRHVLIAASADFDARRGGDLVLYFHGNQATLARDVVDRQQAPQQVAESGLNGVLI
                     APQMAVNALDSSAGRFWRPGQLAAFLNEADQRLAGLYGGSAGEFRRLPVVIVAYSGGY
                     LPAAYSLAVGGADYRVRGVVLLDALYGEEDKFFDWIRRSRGRAFFVSAYSKSSRDQNE
                     ALKARLIAAGVPVQDHLPDRLGPGVVAFIDAGDAVHDDFVNYAWTSQPLRDLLARVAR
                     "
     gene            complement(1122..3917)
                     /locus_tag="VKU03_00050"
     CDS             complement(1122..3917)
                     /locus_tag="VKU03_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69677.1"
                     /translation="MNDRFAPAKALPASLIAADSDLVLDRRGRLALYLAVGLAAGAVI
                     ALQIDVMRVFSVGSWAHFGSLVVSLAMLGFGLTSAVMCVAKNWFQRHWRGAAGVALAS
                     FGPLAVAANLYVQKLGFNAIFLVSDPAQKWKLLQIFLVDLTPFLAGAVFLGCVFLKSN
                     KTFGRVYFADLAGSGLCGLVFLGAMYLFRPENLIAAPLALWLAAMIAWAFGPGGLRAL
                     APFAALAVLAFGGHFVAAPALGLPRLAVNDYKGVAYARKFPDSKRIYESVSPFGYIEA
                     YSSSYLHFAPGLSDNAAFNLPAVPANAYIAMYIDSDGPIGVIRDLADKDTAYFRFLPM
                     YYPYVIKKDPRVFVVQFGGGISTAVALRSGAKKVTVAEGNRAVLEAFRSKVFKDFTGD
                     ILGRVNKVIDYEGRHYLAHTNQRFDVIDLSLADSTGLSNPGGFAIVEKFPYTREAMET
                     YMRALAPGGVLSVTLWNKEEPPKSILKLYATMAAAAERVDPAHIADSFFVVSSYLSTA
                     TVLYKRGGFAEDEIAKLRKHTKAMSFDEIYSPGFFYDGTQTDRTLNGYAAQIFSTSSP
                     AGGPTAPGAAAEGGDNGGASDPSGPSDDNGAGGGGDDNGVMPATVMGRLAWASLINGE
                     WPQIAKRYVFNTHILTNDTPYFAAYVKTKDLPRTLDFDSFADSLNWKALGDWDLKTFW
                     RDLDFQRLFDRLEPLQDQWGYLLIWATLGVAALTASVLLAIPVLWGWRAIFSKTPGKL
                     LTIVYFACLGAGYIMVEVGLIAHFVMALGNATVSASVLITGMLVFSGLGSLVSERILP
                     RMRIAMPPIFLAIGGLLIGYALFLDHALDAIGALPYGVRLVLAFAFIAPPAFLMGFPM
                     PTAMTTLGRLGKDHMFIWAWGVNGCFSVIGAAAAPVIATNFGLGAVIEIAGCAYLLAL
                     PAFFGVISAGRSKQRFA"
     gene            complement(3991..4860)
                     /locus_tag="VKU03_00055"
     CDS             complement(3991..4860)
                     /locus_tag="VKU03_00055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HLJ69678.1"
                     /translation="MQRRQFLAGSAGLLASALVPRLALANVPTPFTFDMVPPTGSREE
                     FVKWGVAQRGEDPKFLGERFDRYGVLVRNEDLWDDRNKHAFLLTPREEFVLKQNLSRA
                     YDHAFLDIGYGVTISGPHIVGRMTSTIDVKKGEKVLEIGTGSGYQSAYLAHLTDNVHT
                     IEIIKPLAERTRGIYDALIDRGYTEFKAIATKNADGYYGWEEAAPFDKIIVTCGIDHI
                     PPPLLQQLKPNGIMVIPVGPPGAQRVLKVVKSQAEDGTITVARSDIYHGRIVPFVPFT
                     KLEGDVIKGTHNE"
     gene            5120..5881
                     /locus_tag="VKU03_00060"
     CDS             5120..5881
                     /locus_tag="VKU03_00060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019964419.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter permease"
                     /protein_id="HLJ69679.1"
                     /translation="MRSLANPTAFLAFAQRVLPWLWAATACLFAIGLWLALFVAPPDY
                     QQGETSRIMYLHVPAAWMGMLVYAAMALAALGTLVWRHPLADAAQKAAAPLGAGFTFI
                     CLATGSLWGKPMWGTYWVWDARLTSVLVLFILYLGLIALWRAIDEPGRAARAAAILTL
                     VGVVNIPIIKFSVDWWNTLHQPASVFRLGGPTIAPLMLWPLLVMAVAFLALFVALHLM
                     AIRNEILRRRVARLTMAAATRRDAFASTLAAEARL"
     gene            5878..6036
                     /gene="ccmD"
                     /locus_tag="VKU03_00065"
     CDS             5878..6036
                     /gene="ccmD"
                     /locus_tag="VKU03_00065"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmD"
                     /protein_id="HLJ69680.1"
                     /translation="MTEPAHIGFVIAAYAVAAIVLVGMVVAVFADYRAQRKALRRLES
                     RERQARLD"
     gene            6032..6619
                     /locus_tag="VKU03_00070"
     CDS             6032..6619
                     /locus_tag="VKU03_00070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbE family thiol:disulfide interchange protein"
                     /protein_id="HLJ69681.1"
                     /translation="METPIGAAQTGAPQRRLLALLPLLVVLALAALFYARLFAGDASR
                     IPSALIGKSAPPIDLKGLDGGPGLSDADLRAGHVSLVNVFATWCLPCHAEHETLMALA
                     ADKDLAAKGVKLYGVAQKDDPENLRRFLGAKGDPYAKIGLDPDNRAGIDWGVYGVPET
                     FIIKGDGVIAYKLIGPMDPQILAAIVKPQIEKAMN"
BASE COUNT         1213 a         2184 c         2232 g         1116 t
ORIGIN      
        1 attcccgcga aaaagcgaga atccagaaac ctccgcgctc gataggccgc gttggattcc
       61 cgcttgcgcc ggaatgagag ttgtattcta ccgcgcgacg cgcgccagta ggtcgcgcaa
      121 cggctggctg gtccaggcgt agttgacgaa atcgtcatgg acggcgtcgc cggcgtcgat
      181 gaacgcgacg acgcccgggc cgagacgatc gggcaaatgg tcctgcaccg gcacgcccgc
      241 cgcgatcagc ctggctttca gcgcttcgtt ctgatcgcgc gacgatttcg aataagcgct
      301 gacgaaaaag gcgcggccgc gcgagcggcg aatccagtcg aagaacttgt cctcttcgcc
      361 atagagcgcg tcgagcagca cgacgccgcg cacccggtag tccgcgccgc cgaccgccag
      421 cgaataggcg gccggcagat agccgccgct ataggcgacg atgaccaccg gcaagcgccg
      481 gaactcgccg gccgagccgc catagaggcc ggcgaggcgc tggtccgctt cgttcaggaa
      541 agccgccaat tggcccggcc gccagaaccg gccggcgctg gaatcgagcg cgttgaccgc
      601 catttgcggc gcgatcagca cgccgttgag gcccgattcc gccacttgct gcggcgcctg
      661 ctggcgatcg acgacatcgc gcgccagcgt cgcctggttg ccgtggaaat agagaacgag
      721 atcgccgccg cggcgcgcgt cgaaatcggc gctcgccgcg atcaggacat ggcggtcgtt
      781 ataggtttcg tcctcccagt agacgccgcc gcgcggcgaa gaatggccca gtcggccatt
      841 cgcattgacg tcgagaaagg gccgtccctc gtcgggaatg gcgccgtgat aggggaaggc
      901 cgagttttgg aactcggcgt aagcgacatt gacgccgcca gcctgcgtga agcccgaact
      961 cgtcggcggc gcggccgcct gcctcaccgg cgccggcccg gcgcagccgg ccagcgccac
     1021 tgcgacagcc agaaaaagcg cgcgtcgttg cgcggcggcc gagccgaagg caacgcccct
     1081 tccatctctc attcgcccct gctccggatt ttttaatccg attacgcgaa tctttgcttt
     1141 gaacgtcccg ccgatattac cccgaaaaag gccggcagag ccagcaggta cgcgcagccg
     1201 gcgatctcga tcaccgcgcc gaggccgaaa ttggtggcga tcaccggcgc cgccgcggcg
     1261 ccgatcaccg agaagcagcc attgacgccc cacgcccaga tgaacatgtg atccttgccg
     1321 agtcggccga gcgtcgtcat ggccgtcggc atcgggaagc ccatcaggaa agccggcggc
     1381 gcgatgaagg cgaaggcgag cacaaggcgc acgccatagg gcagcgcgcc gatcgcatcg
     1441 agcgcgtggt cgaggaacag ggcgtagccg atgagcaatc cgccgatcgc caggaagatc
     1501 ggcggcatgg cgattctcat gcgcggcagg attcgctcgg agacgaggct gcccaagccg
     1561 gaaaagacca gcatgccggt gatcagaacg gaggccgaca cggtggcgtt gccgagcgcc
     1621 atgacgaaat gcgcgatcag gccgacttcg accatgatgt agccagcgcc gaggcaggcg
     1681 aaatagacga tggtcaggag cttgcccggc gttttcgaaa agatcgcgcg ccagccccaa
     1741 aggacgggaa tggcgagcag cacgctggcg gtcaaggcgg cgacgccgag cgtcgcccag
     1801 atcaggagat agccccactg gtcctgcagc ggctcaaggc gatcgaacag gcgctgaaaa
     1861 tccaagtcgc gccagaacgt cttcaggtcc cagtcgccga gcgccttcca attgagagag
     1921 tccgcgaagg aatcgaagtc gagcgtccgc ggcaaatcct tggtcttcac ataggcggcg
     1981 aaatacggcg tgtcgttggt caggatatgg gtgttgaaaa cgtagcgctt ggcaatctgc
     2041 ggccattcgc cgttgatcag gctggcccag gcgagccggc ccatcaccgt cgccggcatg
     2101 acgccgttgt cgtcgccgcc gccgcccgcg ccgttatcgt cgctcggccc gctcggatcg
     2161 gacgcgccgc cattgtcgcc gccctccgcc gcggcgcccg gcgccgtcgg gccgccggcc
     2221 ggactgctcg tcgagaagat ctgggcggcg tagccgttca acgttcgatc ggtctgcgtg
     2281 ccgtcgtaga agaagcccgg cgaatagatt tcgtcgaagg acatcgcctt ggtgtgcttg
     2341 cgcagcttgg cgatctcgtc ctcggcgaag ccgccgcgct tataaaggac ggtcgcggtc
     2401 gacaggtacg aggaaacgac gaagaacgaa tcggcgatat gggcgggatc gacccgctcg
     2461 gccgccgccg ccatggtcgc atagagcttg aggatcgatt tcggcggctc ctccttgttc
     2521 cagagcgtga ccgacagcac gccgcccggg gcgagggcgc gcatataggt ctccatcgcc
     2581 tcgcgcgtat aggggaactt ttcgacaatg gcgaagccgc cgggattgga gaggccggtc
     2641 gaatcggcga gcgacaggtc gatcacatcg aagcgctgat tggtgtgagc gaggtagtgg
     2701 cgcccttcgt agtcgatcac cttattgacg cggccaagga tgtcgccggt gaaatccttg
     2761 aagaccttgc tgcggaacgc ttcgagcacc gctctattgc cctcggcgac cgtcaccttc
     2821 ttggcgccgg agcgcagcgc gacggccgtc gaaatgccgc cgccgaattg cacgacgaaa
     2881 accctcggat ctttcttgat cacataagga tagtacatcg gcaggaagcg gaaataggcc
     2941 gtatccttgt cggcaaggtc gcgaatgacc ccgatcggcc catcgctgtc gatatacatc
     3001 gcgatatagg cgttggccgg caccgccggc aggttgaacg ccgcgttgtc ggagaggccc
     3061 ggcgcgaaat gcaggtacga gctcgaatag gcctcgatat agccgaaggg cgagacgctt
     3121 tcgtagatgc gcttcgaatc ggggaatttg cgagcgtagg cgacgccctt gtagtcgttg
     3181 acggcgaggc gcgggagccc cagcgccggc gcggcgacga aatggccgcc gaacgcgagg
     3241 accgcgagcg ccgcgaaggg cgccagcgcg cgcaggccgc ccggcccgaa agcccaggcg
     3301 atcatcgccg cgagccagag ggcgagcggc gccgcgatca ggttttccgg ccggaaaaga
     3361 tacatcgcgc cgagaaacac caggccgcac aggccggagc cggcgagatc ggcgaaatag
     3421 acgcggccga acgtcttgtt gctcttgagg aagacgcagc cgagaaacac cgcgccggcg
     3481 agaaacggcg tcaggtcgac gagaaagatc tgcagcagct tccatttctg ggccggatcg
     3541 gagaccagaa agatggcgtt gaagccgagc ttctgcacat agaggttggc ggcgacggcg
     3601 agcgggccga acgaggcgag cgcgacgccg gcggcgccgc gccaatggcg ctggaaccag
     3661 ttcttggcga cgcacatgac cgcgctggtc aagccgaagc cgagcatcgc gaggctgacg
     3721 acgagcgagc cgaaatgcgc ccaactgccg accgagaaga cgcgcatcac gtcgatttgc
     3781 agcgcgatca ccgcgccggc ggcgaggccg acggcaagat agagcgcgag ccggccgcgc
     3841 cggtcgagga cgaggtcgct gtccgcggcg atgagactgg ccggcagcgc cttggcggga
     3901 gcgaaacgat cgttcatcgc gcggcctcta gcagcgtcgc tatcccttct ccccgcaagg
     3961 cggggagaag ggaatgccgc gcctcgcccg tcactcgttg tgggttccct tgatcacgtc
     4021 gccttccagc ttggtgaagg ggacaaaggg cacgatccgg ccgtgataga tgtcggaccg
     4081 cgcgacggtg atcgtcccgt cctcggcctg cgacttgacg actttcagga cgcgctgcgc
     4141 gccgggcggg ccgaccggaa tgaccatgat gccgttcggc ttcaattgtt gcagcagggg
     4201 cggcgggata tggtcgatgc cgcaagtgac gataatcttg tcgaacggcg ccgcctcctc
     4261 ccagccgtaa tagccgtcgg cgttcttggt ggcgatcgcc ttgaactcgg tatagccgcg
     4321 atcgatcagc gcgtcgtaga tgccgcgggt gcgctccgcg agcggcttga tgatctcgat
     4381 cgtatggacg ttgtcggtca gatgggcgag ataggccgac tgatagcccg agccggtgcc
     4441 gatctcgagc accttctcgc ccttcttgac gtcgatggtc gaggtcatgc gccccacgat
     4501 gtgcgggccg gaaatcgtca cgccatagcc gatatcgagg aaggcgtggt cataggcgcg
     4561 ggaaaggttc tgcttgagca cgaactcctc gcgcggcgtc agcaggaagg cgtgcttgtt
     4621 gcgatcgtcc caaagatcct cgttgcggac gagaacgccg tagcggtcga agcgctcgcc
     4681 gaggaatttc gggtcctcgc cgcgctgcgc gacgccccat ttgacgaatt cctcgcggct
     4741 tccggtcggc gggaccatat cgaaggtgaa cggcgtcggc acattggcca gggcaaggcg
     4801 cgggaccagc gcgctggcca gcaaaccggc cgacccggcg aggaattgac gtcgttgcac
     4861 caggcgcctc cttggatgaa gaatcgcgac tcaaagccgt gcggccgggc ttcgaagccg
     4921 cccgctgata aaccaaaacg ccccaaggcg cgacaagaga tttggacgaa agctttcctt
     4981 gccgccgatt gtcgcgattg tgtggcggcc ggcgcgagcc gggtttcggc ccgttaagga
     5041 acggtttggc cggcgcggct attgtcctga agcgccgcct cattgccgcc gctccggagg
     5101 aggaataaaa gccccgctta tgcgcagcct cgccaatccg acggcttttc tcgccttcgc
     5161 ccagcgggtc ttgccctggc tgtgggcggc gacggcgtgc ctgttcgcca tcggcttgtg
     5221 gctcgctctt ttcgtcgcgc cgcccgatta tcagcaaggc gagacgtcgc gaatcatgta
     5281 tctgcacgtg cccgccgcct ggatggggat gctggtctac gccgcgatgg cgcttgcggc
     5341 gctcggcact ctagtgtggc gccatccgct cgccgacgcc gcgcagaagg cggccgcgcc
     5401 gctcggcgcc ggcttcacct tcatctgcct cgccaccggc tcgctctggg gcaagccgat
     5461 gtgggggacc tattgggtct gggacgcgcg gctgacctcg gtcctggtcc tgttcatcct
     5521 ttatctcggt ctgatcgcgc tgtggcgcgc cattgacgag ccgggccggg cggcgcgcgc
     5581 cgccgccatc ctgacgctgg tcggcgtcgt caacattccg atcatcaaat tctcagtcga
     5641 ctggtggaac acgctgcatc agccggcctc tgtgttccgg cttggcggcc cgaccatcgc
     5701 gcctttgatg ctttggccgc tcctcgtcat ggctgtcgca ttcctggcct tgttcgtcgc
     5761 cttgcacctg atggcgatcc gcaacgagat tttgcgccgc cgcgtcgccc ggctcaccat
     5821 ggccgcggcg acgcgacgcg acgcgtttgc gtcgaccctc gccgccgagg cccgcctatg
     5881 accgaaccgg cccatatcgg cttcgtcatc gccgcctacg ccgtcgccgc gatcgtgctt
     5941 gtcggcatgg tcgtcgccgt cttcgccgac taccgcgccc agcggaaggc gctgcgacgg
     6001 ctcgaaagcc gcgaaaggca ggcgcgcctt gattgagacg ccgatcggcg cggcgcagac
     6061 cggcgcgccg cagcgccgct tgctggcgct gctcccgctt ctcgtcgtcc tggcgctggc
     6121 cgccttgttc tatgcccgcc tcttcgccgg cgacgcctcg cgcatcccct ccgcgctgat
     6181 cggcaagagc gcgccgccga tcgatctgaa aggcctcgac ggcggcccgg gcctttccga
     6241 cgccgatttg cgcgccggcc atgtctcgct ggtcaatgtc ttcgccacct ggtgcctgcc
     6301 atgccatgcc gagcacgaga cgctgatggc gctggccgcc gacaaggacc tcgccgccaa
     6361 aggcgtgaag ctttatggcg tggcgcagaa ggacgatccg gaaaacctcc gccggtttct
     6421 cggcgccaag ggcgatcctt acgccaagat cggtctcgat cctgacaacc gcgccggcat
     6481 cgactggggc gtctatggcg tgccggaaac gttcatcatc aagggcgacg gcgtcatcgc
     6541 ctataagctg atcggcccga tggacccaca aattctggcg gcgatcgtca agccgcagat
     6601 cgagaaagca atgaactgag ccgccctgcg cccgtcattg caagcgcagg gaagcaattc
     6661 cgtcgtaggc ggcgctgttg tctcggaata gattgcttcg ctcgcggata agagcccccg
     6721 cctcaattca ccacccgctt cacat
//
LOCUS       DATGJW010000004         3207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_104323, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000004 DATGJW010000000
VERSION     DATGJW010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3207
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104323"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VKU03_00075"
     CDS             <1..445
                     /locus_tag="VKU03_00075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLJ69682.1"
                     /translation="VLLARGARRVHAVDVGHGQLDPRLRADPRVVVHEGLDARKIAPS
                     LFAAPPEAIVCDVSFISLRLILPAILPLAAKSAWLAALIKPQFEAGRERLRKGAVKDP
                     AIQREICDAIVACLETLGWERVGVIPSPIRGGDGAEEFLLGARHG"
     gene            438..1706
                     /locus_tag="VKU03_00080"
     CDS             438..1706
                     /locus_tag="VKU03_00080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLJ69683.1"
                     /translation="MDEAALADVTIAVLGRRGEGVAERAQGPLYIPYALPGERVGARV
                     SGERGHLIEIRQASAHRARPICRYFGDCGGCAAQHMDAELYAQWKRGLVVRALAQAGV
                     AAEIAPLVDAHGEGRRRATFHARYADGAVAVGYMQARAHRIVAIEECPILAPGMADAL
                     ASARDIAKALRPLAKPLDLSIAAGLTGLDIDLRGAGPLDFGARQELVALAERRDLARL
                     SNHGEIFVERRPPEIAMGKARVKPPPGAFLQATEEGERMLAALAIAAVRGERIADLFS
                     GAGAFALRLAESRNVHAVDIDSAALAALARAARESQGLRPISTEARNLFERPLTRQEL
                     GRFDAVLFDPPRAGAAAQAAEIAASGAPSVVAVSCNPATFARDAKILLNGGYQARSVT
                     PIDQFRFSPHVEIVGVFARPAKKRARGLLG"
     gene            1703..3130
                     /locus_tag="VKU03_00085"
     CDS             1703..3130
                     /locus_tag="VKU03_00085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLJ69684.1"
                     /translation="MNDATAEKRLAALAAIVGPAHVVADPERLASHLVEPRGLYHGRA
                     LAMARPGSTEEIARLLAYCNETRLPVVPQGGNTGLVGGQIPDQSGGAIVLSLQRMNRV
                     REVDPDSNSMVVEAGVTLAAAQQAAEKADRLFPLSLASEGSCTIGGNLSTNAGGVGVI
                     AYGNARDLALGLEVVLANGAVINGLSKLRKDNTGYDLKDLFIGAEGTLGIIAAASLKL
                     FPRPRAMATAFVGAPDPAAALKLLALAREAVGPGVTSFELLPRIGLDFVLRHTPGARD
                     PLASPHPWYVLIELSSQQSGRLDDALAEILAAAVEQGIAADAAIAATLEQRKAFWRLR
                     EEMSDAQRPEGGSIKHDVSVPVGATADFIAEASAAVLAFMPDARVVAFGHLGDGNIHF
                     NVRGPAEADSAQWFDTNGDAVSRLVDDLIVAAGGSISAEHGIGQFKLAEFARTAGPAR
                     LSALRAVKRALDPEFKMAPGVLFEP"
BASE COUNT          467 a         1147 c         1095 g          498 t
ORIGIN      
        1 tgtcctgctc gcccgcggcg cgcgccgcgt ccacgccgtc gatgtcggcc acggccaact
       61 cgatccccgc cttcgcgccg acccgcgcgt cgtcgttcat gaggggctcg acgcgcgcaa
      121 gatcgcgcca agtcttttcg ccgcgccgcc cgaggcgatc gtctgcgacg tgagcttcat
      181 ttcgctgcgg ctcatcctcc ctgccattct gccgctcgcg gcgaaaagcg cctggctcgc
      241 cgcgctgatc aagccgcaat tcgaagcagg ccgcgagcgc ttgaggaaag gcgccgtcaa
      301 agacccggcc atccagcgcg aaatttgcga cgcgatcgtc gcctgcctcg agactttagg
      361 ctgggagaga gtcggcgtta tcccttcgcc gattcgcggc ggcgacggcg ccgaggaatt
      421 cctgctcgga gcgcggcatg gatgaggccg cgctcgcgga cgtcacgatc gccgttctcg
      481 gccgccgcgg cgaaggcgtc gccgagcgcg cgcaggggcc gctttacatc ccctacgctt
      541 tgccgggcga acgcgtgggc gcgcgcgttt ccggcgaacg cggccatctc atcgagatca
      601 ggcaagcgag cgcccatcgc gcccggccga tctgccgcta tttcggcgat tgcggcggct
      661 gcgcggcgca gcacatggac gccgaacttt atgcgcagtg gaaacgcggc ctggtcgtgc
      721 gcgcgctggc ccaggcgggc gtcgcggccg agatcgcgcc gctcgtcgac gcgcatggcg
      781 agggccgccg ccgggcgacc ttccacgccc gctatgcgga cggcgccgtc gcggtcggct
      841 acatgcaggc gcgtgcgcat cggatcgtcg ccatcgagga atgtccgatc ctggccccgg
      901 gcatggcgga cgccctggcg agcgcgcgcg acatcgcaaa ggcgttgcgc ccgctcgcca
      961 agccgctcga tctctcgatc gcggccggcc tgaccgggct cgacatcgat ctgcgcggcg
     1021 ccggtccgtt ggatttcggc gcccggcaag agcttgtcgc cctggccgag cggcgcgatc
     1081 tcgcccgtct ctccaatcac ggcgaaatct tcgtcgagcg ccggccgccg gaaatcgcca
     1141 tgggcaaggc gcgggtgaag ccgccgcccg gcgcgtttct tcaggcgacc gaggaaggcg
     1201 agcgcatgct cgcggccttg gcgatcgccg ccgtcagggg cgagcgcatc gccgatctct
     1261 tcagcggcgc cggcgccttc gccttgcgcc tcgccgaaag ccgaaacgtc cacgcggtcg
     1321 acatcgacag cgccgcgctg gcggcgctcg cccgcgcggc gcgcgagtcg cagggcttgc
     1381 ggccgatctc gaccgaggcg cgcaaccttt ttgagcggcc gctgacgcgg caggagctcg
     1441 gccgtttcga cgccgtcttg ttcgatccgc cgcgcgccgg cgccgcggcg caggcggccg
     1501 aaatcgcggc gagcggcgcg ccctcggtcg tcgccgtttc gtgcaacccg gcgaccttcg
     1561 cgcgcgacgc gaaaatcctg ctcaacggcg gctaccaggc gcgatcggtc acgccgattg
     1621 accagttccg cttttcgcct catgtggaaa tcgtcggcgt gttcgcgcgg ccggcgaaga
     1681 aacgcgcaag aggacttctc ggatgaacga cgcgacggcg gagaagcgtc ttgccgcact
     1741 cgccgcgatc gtcgggccgg cccatgtcgt cgccgatccg gagcggctgg cgagccatct
     1801 cgtcgagccg cgcggcctct atcacggccg cgcgctggcg atggcgcggc cgggatcgac
     1861 cgaggagatc gcccgcctcc tcgcctattg caacgagacg cgcctgcccg tcgtgccgca
     1921 aggcggcaat accggcctgg tcggcggcca aattccggac cagagcggcg gcgcgatcgt
     1981 cctctcgctg caacggatga accgggtgcg cgaggtcgat ccggattcga attcgatggt
     2041 cgtcgaagcg ggcgtgacgc tcgccgccgc gcaacaagcg gccgagaagg ccgatcgcct
     2101 gtttccgctc tcgctcgcct cggaaggctc ctgcacgatc ggcggcaatc tttcgaccaa
     2161 tgccggcggc gtcggcgtga tcgcctatgg caatgcgcgc gatctcgcgc tcggcctgga
     2221 agtcgtgctc gccaacggcg cggtgatcaa cggcctcagc aagctgcgca aggacaatac
     2281 cggctacgac ctgaaggacc tgttcatcgg cgcggaaggc acgctcggca tcatcgccgc
     2341 cgcgtcgctg aagctgtttc cgcggccgcg cgccatggcg accgcgttcg tcggcgcccc
     2401 cgatccggcc gccgccctga agctactcgc cttggcgcgc gaggcggtcg gccccggcgt
     2461 gaccagcttc gaattgctgc cgcggatcgg tctcgatttc gtcctccgcc atacgccggg
     2521 cgcccgcgat cccttggcgt cgccgcatcc atggtacgtc ctgatcgagc tctcctcgca
     2581 gcagagcggc cgcctcgacg acgcgctcgc cgaaattctc gcggcggcgg tcgagcaagg
     2641 aatcgcggcc gacgccgcga tcgccgcgac gctcgaacag cgcaaggcgt tttggcgcct
     2701 gcgcgaggag atgtccgacg cccagcgccc ggaaggcggc tcgatcaagc acgatgtcag
     2761 cgtgccggtc ggcgcgacgg cggatttcat cgccgaggcc agcgcggcgg tcctcgcctt
     2821 catgcccgac gcgcgggtcg tcgctttcgg ccatctcggc gacggcaata tccatttcaa
     2881 cgtgcgcggc ccggccgaag ccgattcagc gcaatggttc gacaccaacg gcgatgcggt
     2941 gtcgcggctg gtcgacgatc tgatcgtcgc cgcaggcggc tcgatctcgg ccgagcatgg
     3001 cattggccag ttcaagctgg ccgaatttgc ccgaaccgcc ggtcccgccc ggctctcggc
     3061 gctacgcgcg gtgaagcggg cgctggaccc ggagttcaag atggcgcccg gcgtactctt
     3121 cgagccgtga gttcagatca cgatcaacgc agagcccgca gaaaagaagt tgggcgttac
     3181 gtcaacaagg cggcgacgcc cgcgagc
//
LOCUS       DATGJW010000005        21726 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_623292, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000005 DATGJW010000000
VERSION     DATGJW010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 21726)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21726)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21726
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623292"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(145..1320)
                     /locus_tag="VKU03_00090"
     CDS             complement(145..1320)
                     /locus_tag="VKU03_00090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003549244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ69685.1"
                     /translation="MLRTLLAGGVVAGAIFVAGAALADDNTVTIGMILPMTGASASTG
                     KQERAGAELYIKQHGDTIAGKKIQLVVKDDTGAPDVTKRLAEELIGNDKAKVLIGFGL
                     TPLALAVAPIATAAKVPEVVSAAGTAMITEKSPFIVRTSFTLPQAAQPMAEWCLKNNI
                     HKVVTLVTDYGPGIDAEKWFAEPFAKGGGTVIEKIRVPLKNPDFAPFLQRVKDDKPDA
                     VFVFVPSGFGAAFMKEFVERGLDKSGIKLIGTGDVTDDDLLPSMGDAAIGAVTTHHYS
                     AAHDSPENKAFVADFEKANNGMRPNFMAVGAYDGMALIYHALEATKGSTDGQALIDAM
                     KGAKWMSPRGPVEIDPQTRDIIQNIYVRKVEKVNGQLYNVEFDKIEAVKDPFKAAGN"
     gene            1634..4012
                     /locus_tag="VKU03_00095"
     CDS             1634..4012
                     /locus_tag="VKU03_00095"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HLJ69686.1"
                     /translation="MALRIVKWAAIVVAALIVLAVGGVAALFYRAMPDYSGSASLPGL
                     SADVRVYRDAYAVPHIFAASWDDAARALGYIHASERLYEMEIQRRAGQGRLAEIVGPD
                     LLGVDKFIRTLGFYRLAESSFAALSPEAQKTLQAYADGVNAFLASHRGRLPPEFLLLG
                     DNPEPWRPADTLVIGKLLSLQLSNNYGLEMDRAMLATKLPPDQARLLFPAPPAGTPIT
                     TEPAARADHAEAPSPLEKLGEILPFAHGASNEWVISGAHTVTGKPILANDPHLGIEAP
                     IVWYLARIVTPGGWVKGASLPGSPLVVLGQNDHIAWGFTNTGSDVQDLFVETVDPKDS
                     SRYLTPDGAKPFETREETIHIKGAPDVALKIRATRHGPVLSDVVGKMAAIAGPGKVMA
                     LAYNALGGRDTTIEAMLRIDHAKDWSQFRDALKLFQTPEQNMVFADAGGNIGFISPAL
                     LPVRKSGDGLAPSDGASGATDWIGYAPFDKMPQIFNPPAGFIFNANNAIVPPDNDPYL
                     GQDWEEPYRARRIQQFFDRLGDKHSLDTSEAMQGDIVSLAAKDLLPVVARATASTDQA
                     RQALALLAKWDGAMDKDRPEPLIFDAWMREMRLILVNEKTGLPLSELGPYDATAITSL
                     VKDHPQWCDGIKGPDPDCRATITRALDQALALLSKRDGADMTKWRWGAEHISVLTHKL
                     YSHVPILDRLSDLSVASSGDFYTLNRGGAHDPPAARPFARLHAAGYRGIYDLGDPAKS
                     RFMITTGESGHIFSPHYGDLVPLWNDVRAITLAGSEEDLKRRGASELVFSTK"
     gene            complement(4172..4984)
                     /locus_tag="VKU03_00100"
     CDS             complement(4172..4984)
                     /locus_tag="VKU03_00100"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLJ69687.1"
                     /translation="MELKAAPIDALSSAALHLTPTTPVVVFGPEARLDIHDFAVRNHL
                     DPAMVAREHAASGVIHCGNARGAGQLTLSNSVITTAAHVFFDERGELRGDNGHCVFTA
                     IVGGQEITTTIAVSSILAGDKKPYAHPAVNDWAVARLMRPVTGATPYALGGRAQAGSP
                     IEFVARGHSDWGGGKAMSLEDCRLRNQLSTGGQGTREFSFDCDAGVGASGGALLAHGD
                     QLVGVFVGYRSIAPEKAMPFSSQHYNFAVSVDGAFRKAVETVAATPAAISSR"
     gene            5225..6397
                     /gene="zapE"
                     /locus_tag="VKU03_00105"
     CDS             5225..6397
                     /gene="zapE"
                     /locus_tag="VKU03_00105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapE"
                     /protein_id="HLJ69688.1"
                     /translation="MSATLKTQYDALVAAGRLEADPAQSAVIEELAALAERLERYAPA
                     RRPNGLARLIGARPAEPPRGLYLYGPVGRGKTLLMDLFFEAAPTGAKRRVHFHAFMAD
                     VHQRIHEWRQRHKAGHATGDDPIAPVAADLAAEAWLLCFDEFSVNDIADAMILGRLFA
                     ALFAAGVVVVATSNVAPDDLYKDGLNRALFLPFIALLRERLDICELQGRADYRLEKLV
                     RAPVYYHPADAAAKAALGRAFERLTGHARGAPSTIELLGRSLSIPEADDGVARFGYAD
                     LCRQPLGATDFLAIARRFHTIIIDDIPILDPERRDEVKRFINLVDTLYDERVKLVASA
                     AAEPADLYSGGDGFEAFEFARAASRLVEMRSADYLALAHGHAGASVSGDLGGLVET"
     gene            6457..7065
                     /locus_tag="VKU03_00110"
     CDS             6457..7065
                     /locus_tag="VKU03_00110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ69689.1"
                     /translation="MPRSQSAEPVEARVLEIACAHVRKFGAGRTTIVGVAREAGMTHA
                     NVYRYFPSKEALFEEMTAAWLRPLETRLREAADGADPAYDKLERMLIAIHRAYRQKLE
                     SDAPLFDLLVEALQKGKSSARKHRARVQADLQRVVEEGIAAGAFAMTDRRRALSLVFD
                     AAHRFIHPLAVRLDAETPAAALEARFERIIALALRALRSGRV"
     gene            7142..7879
                     /gene="truA"
                     /locus_tag="VKU03_00115"
     CDS             7142..7879
                     /gene="truA"
                     /locus_tag="VKU03_00115"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HLJ69690.1"
                     /translation="MPRYKLTLEYDGAPFVGWQRQANGLSVQQALEEAILAASGEEVV
                     ANGAGRTDAGVHATGQVAHVDLAKDWRGFRLSEALNALLVPKPIAVLAAERVADDFDA
                     RFSARMRRYLYRIVNRRAPLALERERAWRVKRPLDAEKMREGAALLVGKHDFSTFRDS
                     QCQAASPVKTLSSFDIERIGDEIVMRLSARSFLHRQVRSMVGSLEHVGSGKWSVGDLR
                     AALEARDRARCGQVAPAAGLYLAKVEY"
     gene            complement(8054..9220)
                     /gene="dapE"
                     /locus_tag="VKU03_00120"
     CDS             complement(8054..9220)
                     /gene="dapE"
                     /locus_tag="VKU03_00120"
                     /EC_number="3.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009014 - succinyl-diaminopimelate
                     desuccinylase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinyl-diaminopimelate desuccinylase"
                     /protein_id="HLJ69691.1"
                     /translation="MPQPPLAALDIARALIACPSVTPDEAGALTYLRGILVEAGFAAE
                     IVSFDAPGAARVDNLYARFGRGAPHLVFAGHTDVVPPGDPAKWRFDPFSGEIADGMLW
                     GRGACDMKGGVAAAVAAALRFVVRKEGVKGSVGFLITGDEEGAAVNGTVKLLDWALAK
                     GERFDHCILGEPTSREKLGDMIKHGRRGSLTGRLTLIGKQGHVAYPHLADNPLRALAP
                     VLAALTEPPLDSGTPAFDASNLEATSVDVGNRAANVIPGEARIVFNIRFNDIWTPETL
                     RAEIEKRVARAAGRMRYELTFDPTNATAFLTPSGAFTGLVADAVQDVVGRRPTLSTSG
                     GTSDARFIKNACPVVELGLVGETMHGVDERAPVDDIEALSQIYERILEKYFAGF"
     gene            complement(9457..10053)
                     /locus_tag="VKU03_00125"
     CDS             complement(9457..10053)
                     /locus_tag="VKU03_00125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ69692.1"
                     /translation="MPNDVVDWIRKALLATGKTRSGLAKALGRSPSAVTDLLNGARRL
                     RAEEIVVAANYLGVEPPPFLGGGRRPPSGLLVPVVGFVGAGAEAHFYAEGQGPFDEVP
                     APDGASSQTVAVQVRGHSLGALFDNWLVFYDDVRDPPGDDLVGHMCVCGLSDGRVLIK
                     ALKRSQIAGLWTLLSNVEPPIYDVALDWAAQVSAMRPR"
     gene            10189..10413
                     /locus_tag="VKU03_00130"
     CDS             10189..10413
                     /locus_tag="VKU03_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69693.1"
                     /translation="MGKVRLRRRAPKIDTARMEAIVACAEHLRDLKRVHGAPPPDIEV
                     ATSGTPQRLAPMATNSYCTSPAELCAELVK"
     gene            10410..11006
                     /locus_tag="VKU03_00135"
     CDS             10410..11006
                     /locus_tag="VKU03_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69694.1"
                     /translation="MSGRASTRRPTRSRRDAIAAPERLEATPERLARARDCGQEIALD
                     RERRTRIDDPFDAMRARRALAPQDSRLNDLRWLIGEALRRLHHRAGLDSLRAMAPDRV
                     GLVAPGPRPGLPLSDLALHARDKLRACESLAGAAAWPVLRRIVIEGANLRDCRPLIAE
                     VSTPWRADAILADRLRCGLDALGGHLGVTAKRSGFASG"
     gene            11098..11394
                     /locus_tag="VKU03_00140"
     CDS             11098..11394
                     /locus_tag="VKU03_00140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69695.1"
                     /translation="MTRSLASKALAAAAGASLFALTLAAPVPASAFTLAAPDLGHSFA
                     DNGQVHKAWWDRWGRWHPNYWGPRYGWRPGWGYGGWGPHRHCWMSPWGWRCRWY"
     gene            complement(11545..12291)
                     /locus_tag="VKU03_00145"
     CDS             complement(11545..12291)
                     /locus_tag="VKU03_00145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YebC/PmpR family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLJ69696.1"
                     /translation="MAGHSQFKNIMHKKGKQDAIRSKLFSKLAREITVAAKLGLPDPA
                     MNPRLRAAVIAARAENMPKDNIERAIKKAAGGDAETYDEVRYEGYAPGGVAIIVEALT
                     DNRNRTAGEVRSSFAKAGGSLAETGAVSFMFDRVGLIVYDRKVASDDAMLEAAIEAGA
                     DDVASSGETHEIVTSIESLRESQKALEAQFGEPQKAAIVWRPQNSIAVDDEAGEKILK
                     LVDALEENDDVQNVYANFEVSDALIAKMQG"
     gene            complement(12335..13516)
                     /locus_tag="VKU03_00150"
     CDS             complement(12335..13516)
                     /locus_tag="VKU03_00150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ69697.1"
                     /translation="MDPRTNPYAPGAGSPPPELAGRDDTIERAAIALDRIRMGRPARS
                     LILVGLRGVGKTVLLNHIRRDAEARGIIGVSIETPEDRSLPSLLVPALRIGLLQLSRI
                     EALRARLDFAGRVLASAAKAFKIKFQDVEFNFDAISEMGIADSGDFERDLSDLFVAVG
                     SAARERQTALVLAIDELQYVPETQLSALIAALHRASQEQLPVTMIAAGLPQLMGLAGR
                     AKSYAERLFEFAQIGALGQDDATLALCKPAEREGVEFSPGAIVEILRRTHGYPYFLQE
                     WGKHAWNIAKVSPIETVDAEKATESALADLDAGFFRVRFDRLTPAEKRYMRAMAELGS
                     GPHRSGDIAEVLGKKVTNVAPVRSSLVAKGMLYSPAHGDNAFTVPLFDGFMKRTIPLA
                     K"
     gene            complement(13583..14398)
                     /locus_tag="VKU03_00155"
     CDS             complement(13583..14398)
                     /locus_tag="VKU03_00155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00282 family metallophosphoesterase"
                     /protein_id="HLJ69698.1"
                     /translation="MRLLFIGDVVGRSGRTIVNQQLPLLREKWRLDFVVVNAENAAGG
                     FGVTEAICQELIDSGADAVTLGNHAFDQREALIYIERQPRLIRPANYPPGTPGRGANL
                     IEAKDGRKMLVVNMMGRTFMDALDDPFARIDQELAACPLGAGCDAAIVDFHAEATSEK
                     QAFGLYVDGRVSLVVGTHTHTPSADHRILPGGAGFITDAGMSGDYDSVIGMDKEEPLR
                     RFTRRLPSARFEPALGPATLCGVAVETGEGGLARKIAAVRIGGILTPATPDFW"
     gene            complement(14405..14992)
                     /locus_tag="VKU03_00160"
     CDS             complement(14405..14992)
                     /locus_tag="VKU03_00160"
                     /EC_number="6.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030272 - 5-formyltetrahydrofolate
                     cyclo-ligase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-formyltetrahydrofolate cyclo-ligase"
                     /protein_id="HLJ69699.1"
                     /translation="MTTAPAAAKADLREAALARRRALGQADRAKFSARLAREGLRLAR
                     RTGARAVSAFHAFPDEPDTGPLLAALAEADFVTLLPITGTRGAPLVFRRWRPGEPTRP
                     GRMNIPEPLEDAPARDPDLLFVPLACFDRRGHRIGFGAGYYDLTLRALRAKGRAVAVG
                     VGYSVAECPILPDEPHDERLDYVLTESELIDCRDS"
     gene            complement(15022..15175)
                     /gene="ssrS"
                     /locus_tag="VKU03_00165"
     ncRNA           complement(15022..15175)
                     /ncRNA_class="other"
                     /gene="ssrS"
                     /locus_tag="VKU03_00165"
                     /product="6S RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00013"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00013"
     gene            complement(15223..15507)
                     /locus_tag="VKU03_00170"
     CDS             complement(15223..15507)
                     /locus_tag="VKU03_00170"
                     /inference="COORDINATES: protein motif:HMM:NF014019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal-binding domain-containing protein"
                     /protein_id="HLJ69700.1"
                     /translation="MQISESQQLDDGRQTFAIGRIKAAGAWRAANAAGGEADRQAALR
                     KLIREAEECGADAIVGLEFKMDGVMGADIDGVALRRVVATGLAVRFAMAA"
     gene            complement(15733..17877)
                     /gene="pnp"
                     /locus_tag="VKU03_00175"
     CDS             complement(15733..17877)
                     /gene="pnp"
                     /locus_tag="VKU03_00175"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HLJ69701.1"
                     /translation="MFDIQREELNWCGRKLTLETGRMARQADGAVFASWGETSLLATV
                     VAAKAPKPGVDFFPLTVNYQEKAFAAGRIPGGYLKREGRPSDRETLTSRLIDRPIRPL
                     FPDGFRCDTQVIVTVLSHDLENDPDVLALVAASAALTLSGIPFMGPIGAARVGHVGGQ
                     LKVNPTIDEMKESALDLVVAGTAEAVLMVESEAKELSEETMLQAVMDGHRAFQPVIEA
                     IIRLAEKAAKEPRELVVPDKSAVLNAVLEHGESELRAAYAITAKQERYAAVDAVKAKV
                     MAALAPDEGEAKFSKEEVAEAFHEAQAKVVRWNILDQKRRIDGRDLTTVRPILAQVGV
                     LPRTHGSALFTRGETQALAVATLGTGEDEQYVDSLEGTYKERFMLHYNFPPYSVGETG
                     RMGSPGRREIGHGKLAWRAIHPMLPTAAEFPYTIRVVSEITESNGSSSMATVCGSSLA
                     LMDAGVPMKRPTAGIAMGLILEDKRFAVLSDILGDEDHLGDMDFKVAGTTEGVTSLQM
                     DIKIAGITEEIMKVALAQAKDGRLHILGEMAKALSGSRAELGEFAPRIETIKIPTDKI
                     REVIGSGGKVIREIVEKTGAKINIEDDGTVKIASADGAKIKAAINWIKSIASDPEVGM
                     IYDGTVVKTVDFGAFVNFFGSRDGLVHISQLAKGRVAKTTDVVKEGDKVKVKLLGFDD
                     RGKIRLSMRVVDQQTGADLEKQQAAESASQSA"
     gene            complement(18070..18339)
                     /gene="rpsO"
                     /locus_tag="VKU03_00180"
     CDS             complement(18070..18339)
                     /gene="rpsO"
                     /locus_tag="VKU03_00180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HLJ69702.1"
                     /translation="MSISVERKQALVKEYAVKAGDTGSPEVQIAILTERIANLTEHFK
                     THVKDTHSRRGLLKMVSQRRTLLDYVKRQDEARYKSLLERLGIRR"
     gene            complement(18564..19043)
                     /locus_tag="VKU03_00185"
     CDS             complement(18564..19043)
                     /locus_tag="VKU03_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69703.1"
                     /translation="MRDWLKILHAAPLGMMSLLAVAIVLYGALLANSGAPPAGGEEAY
                     SQAWAGFLLTLGLWSVLALMLAVGAAKGRMPVWAALAALLLHPISGLAAFVALDAASR
                     RVGLGAVLLVLLPLPIASYAFWARLPQWREAYPPGLASAVAWGAVALLSLAAFATGL"
     gene            19300..20322
                     /locus_tag="VKU03_00190"
     CDS             19300..20322
                     /locus_tag="VKU03_00190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69704.1"
                     /translation="MTRLDDKLARIRDGKYRRADFIIADAKDPDMGPSMAAAGPARQK
                     DGAATRNRTREEFLGQIRAIVEQDVVDIMLTSASNLDRLARSGVFKGSKIQPAIRAND
                     TTDIWRVRGGAYPQKPSRPFRSANLCRVMWGSATPKVGSPPTGTDLGLYSITFNNDID
                     ADIATLEAFAEFRAEAAALGFRYFLEVFNPNVATGIAPEILPHYVNDCIQRCLAGLTD
                     AERPLFLKVVYNGPKALEEMASYDPSLVVGVLGGGAGTTRDCFELIAQAERYGARVAL
                     FGRKINLAESPLAMVTHMRAVADGAMSPSEAVKAYHGALQKAGIRPDRDLASDDLITE
                     AALKTG"
     gene            20326..21345
                     /locus_tag="VKU03_00195"
     CDS             20326..21345
                     /locus_tag="VKU03_00195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018210085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HLJ69705.1"
                     /translation="MAARRGFVTAGTWCVDNNKLIAAWPEEDTASEVLAIDRQGGGSA
                     CNLAIDMKRLDPGAPVETIGLIGDDENGRFLAAQCDAHGIGRAQMRVVPGEATFSVDA
                     FTVAKGKRTHLFYPGVAKRLSPDDFDVSKTDARILHLGLPGAHQVMDAPWRDEANGWV
                     AVLKAARRNGLETNLELMTIAPERLIALARPCLPHLDYLIVNDFEIGAATGIETRVGG
                     RTDFAAVRRAIGAALASGPMRVVAAHFPEGAIAGARDGATAAIGSLAMPEADIVGANG
                     AGDAFAAGLLYGLHEGWDLRRALALAHCAAAASMRKISTTEGVGTWRDCLALGERWGF
                     RDAPG"
     gene            complement(21514..>21726)
                     /locus_tag="VKU03_00200"
     CDS             complement(21514..>21726)
                     /locus_tag="VKU03_00200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69706.1"
                     /translation="TGRERFFAIASATYAAFHAVDQNIFWTSRHFRLKLFAQQAAGRG
                     LRLQGTAQEGRSEPKRRIQIFRFVSL"
BASE COUNT         3703 a         7142 c         7103 g         3778 t
ORIGIN      
        1 agcatcgaga cgggtacagg gggaccatgc gaagcatggt ggagggggcc gcggcggttg
       61 agatagtcct caggcgccga ggccccctcc accgcgctta cgcgcggtcc ccctcccccg
      121 tcttcgacgg gggaggaagt acgctcaatt ccccgcggct ttgaacgggt ccttcaccgc
      181 ttcgatcttg tcgaactcga cattgtagag ctgtccgttc actttctcga ccttgcggac
      241 gtagatgttc tggatgatgt cgcgggtttg cggatcgatc tcgaccggcc cgcgcgggct
      301 catccatttc gcccctttca tcgcatcgat cagcgcctgg ccgtcggtcg agcccttggt
      361 cgcttcgagc gcgtgataga tcaaagccat gccgtcataa gcgccgaccg ccatgaaatt
      421 cggccgcatg ccgttattgg ctttctcgaa atcggcgacg aaggccttgt tctccggcga
      481 atcgtgggcg gccgaatagt ggtgcgtcgt caccgcgccg atcgccgcgt cgcccatcga
      541 ggggagaaga tcgtcgtcgg tcacgtcgcc ggtgccgatc agcttgatcc ccgatttgtc
      601 gaggccgcgc tcgacgaact ccttcatgaa cgcggcgccg aagccggacg gcacgaagac
      661 gaagaccgcg tcgggcttgt cgtccttcac ccgctgcaga aagggcgcga aatccgggtt
      721 cttcagcggc acgcgaatct tttcgatcac cgtgccgccg cctttggcga aaggctcggc
      781 gaaccatttc tcggcgtcga tgccggggcc gtaatcggtc accagcgtca ccactttatg
      841 gatgttgttc ttcaggcacc attcggccat gggctgagcc gcctgcggca gcgtgaagga
      901 ggtgcggacg atgaacggcg atttctcggt gatcatcgca gtgccggcgg cgctgacaac
      961 ttcgggaacc ttggcggcgg tggcgatcgg cgcgaccgcc aaagcgagcg gcgtcaggcc
     1021 gaagccgatc aggaccttcg ccttgtcgtt gccgatcagc tcctcggcga ggcgcttggt
     1081 cacgtcgggc gcgccggtat catccttgac gacgagttgt atctttttgc cggcgatcgt
     1141 gtcgccatgc tgcttgatgt aaagctcggc gcccgctcgc tcctgcttgc cggtcgaggc
     1201 ggaagcgccg gtcatcggca ggatcatgcc gatcgtgacg gtattgtcgt cggccaaggc
     1261 cgcgcccgcg acaaagatcg cgccggcgac gacgccaccg gcaagcaacg ttctcaacat
     1321 cgtcagctcc tcccaaagcg cctcgcgcca gagcgcaaat ccttggccga acaatcctat
     1381 agcatgggtc gggagacaag agccggagcc tcattctttc gtcaagcgcc gccgcgcctc
     1441 ccagaccgct ggcgctcgat ctaggggagc ggctttcacc cgtcttatcg attcctcgat
     1501 ggtcactatc accgccggtt ataaagacgc acgcagccga cggcttggct ggcggacatg
     1561 gacaaacgag gtcaaaccgg attgatttgg ccggcgtctc gggggcaggc gctttcagtt
     1621 ccagggaccg cggatggctc ttcgcatcgt gaaatgggcg gcgatcgttg tcgccgcgct
     1681 gatcgtcctc gcggtcggcg gcgtcgcggc tctgttctat cgcgcgatgc cggattattc
     1741 gggctcggct tctttgcccg gcctctcggc cgacgtgcgc gtctatcgcg acgcctatgc
     1801 cgtgccgcac attttcgcgg cgagctggga cgatgcggcg cgggcgctcg gctacatcca
     1861 tgccagcgag cggctctacg agatggagat ccagcgccgc gccgggcagg ggcgcctcgc
     1921 cgagatcgtc ggccccgatc ttctcggggt cgacaagttc atccgcacgc tcggcttcta
     1981 ccggctcgcc gaaagcagct ttgcggcctt gtcgcctgag gcgcagaaga cgctgcaagc
     2041 ctatgccgac ggcgtcaacg ccttcctcgc ttcgcatcgc ggccggctgc cgccggaatt
     2101 cctgctgctc ggcgacaatc ccgagccgtg gcggcccgcc gacactttgg tgatcggcaa
     2161 gctgctgagc ctgcagctca gcaacaatta cggcctcgaa atggaccgcg cgatgctggc
     2221 gacgaaactg ccgcccgacc aggcgcgcct cttatttccc gcgccgccgg ccgggacgcc
     2281 gatcaccacc gagccggcgg cgcgcgccga ccatgccgaa gcgccttcgc cgctcgaaaa
     2341 gctcggcgaa atcctgccct tcgctcatgg cgcgtcgaac gaatgggtga tttccggcgc
     2401 ccataccgtg accggcaagc cgatcctcgc caacgatccg catctcggca tcgaggcgcc
     2461 gatcgtctgg tacctcgccc gcatcgtcac gccgggcggc tgggtcaagg gcgcgtcgct
     2521 gccggggtcg ccgctggtcg ttttggggca aaacgaccat atcgcctggg gcttcaccaa
     2581 taccggctcg gacgtccagg accttttcgt cgaaaccgtc gacccgaaag attccagccg
     2641 ctatctgacg ccggacggcg cgaagccgtt cgagacccgc gaagaaacga tccatatcaa
     2701 aggcgcgccg gatgtcgcgc tcaaaatccg cgcgacgcga catgggccgg tcctttccga
     2761 cgtggtcggc aagatggcgg cgatcgccgg tcccggcaaa gtcatggcgc ttgcctataa
     2821 cgcgcttggc gggcgggaca cgacgatcga agcgatgctt cgcatcgacc atgcgaaaga
     2881 ttggagccag ttccgcgacg cgctgaaact gtttcagacg cctgagcaaa acatggtttt
     2941 cgccgacgcc ggcggaaata tcggcttcat cagcccggct ctgctgccgg tccgaaaatc
     3001 cggcgacggg cttgcgccga gcgacggcgc ctcgggcgcg accgactgga tcggctatgc
     3061 gccgttcgac aaaatgccgc aaatcttcaa tccgccggcc ggcttcatct tcaacgccaa
     3121 caacgcgatc gttccgcccg acaacgatcc ctatctcggc caggattggg aggagcccta
     3181 tcgcgcgagg cgcatccagc aattcttcga ccggctcggc gacaagcaca gcctcgatac
     3241 gtcggaagcc atgcaggggg acatcgtctc gctcgcggcg aaggaccttc tgccggtcgt
     3301 cgcccgcgcg acggcctcca ccgatcaggc caggcaggcg ctggcgctac tcgccaagtg
     3361 ggacggcgcg atggacaagg accggcccga gccgctgatt ttcgacgcct ggatgcgcga
     3421 aatgcggctc atcctggtca acgagaagac cggcttgccg ctgagcgagt tgggcccata
     3481 cgacgcgacc gcgatcacgt cgctggtcaa ggaccatccg caatggtgcg acgggatcaa
     3541 gggaccggac ccggattgcc gcgcgacgat cacccgcgcg ctcgatcagg cgctggccct
     3601 gctgtcaaag cgcgacggcg cggatatgac gaagtggcgc tggggcgcgg agcatatttc
     3661 cgtcctgacc cacaagctct acagccatgt gccgattctc gaccggttga gcgatctcag
     3721 cgtcgccagc agcggcgatt tctacacgct caaccgcggc ggcgcgcatg atcctcccgc
     3781 cgctcggccc ttcgcccgtc tgcacgcggc gggctatcgc ggcatctacg atctcggcga
     3841 tccggccaaa tcgcgcttca tgatcacgac cggcgagtcg ggccacatct tctcgccgca
     3901 ttacggcgac ctcgtcccgc tctggaacga cgtcagggcg atcaccctcg ccggctcgga
     3961 agaggatttg aagcgacgag gggcgagcga gctcgtattc agtacgaagt gatttggcta
     4021 tcctcccccg cgaagcgggg gagggggacc gcgcgatagc gcggtggagg gggccacggt
     4081 ctctcaggat aatcccaatc gccggctgcc ccctccaccg cgctgcgcgc ggtccccctc
     4141 ccccgtcttc gacgggggag gataaatacc gtcagcgcga agaaatggcg gccggcgtcg
     4201 cggcgaccgt ctcgaccgcc ttgcggaaag cgccatcgac cgagaccgcg aaattgtagt
     4261 gctgggagga gaacggcatc gccttttcgg gcgcgatcga gcggtagccg acaaagacgc
     4321 cgacaagctg gtcgccatgc gccagaagcg cgccgcccga ggcgcccacg ccggcgtcgc
     4381 aatcgaagga gaattcgcgc gtgccctggc cgccggtcga gagctgattg cgcagccggc
     4441 aatcttcgag cgacatcgcc ttgccgccgc cccaatcgct atggccgcgc gcgacgaatt
     4501 cgatcggcga accggcttgg gcccgtccgc ccagcgcata gggcgtcgcc ccggtgaccg
     4561 ggcgcatcag ccgcgccacc gcccaatcgt tcaccgccgg atgggcgtag ggcttcttgt
     4621 cgccggcgag aatcgacgag acggcgatcg tcgtcgttat ttcctggccg ccgacgatcg
     4681 cggtgaagac gcaatggccg ttatcgccgc gcaattcgcc ccgttcgtcg aaaaacacat
     4741 gggcggcggt ggtgatgacg ctgttcgaca gggtcaactg gccggcgccg cgcgcattgc
     4801 cgcaatgaat gacgcccgac gcggcgtgct cccgcgccac catcgccggg tcaaggtgat
     4861 tgcgcaccgc gaaatcgtgg atgtcgagcc gcgcttcggg cccaaaaacg accactggcg
     4921 tcgtcggcgt cagatggagc gcggccgacg aaagggcgtc gatcggcgcc gccttcaatt
     4981 ccacgcaagc aatgagagaa aaacaagata caacagccgc gcggatgata tgaagcatcg
     5041 gcgagccgcc tcctaaagag aactggcctc caaaggccag tccccggaag cgccggaaac
     5101 cgatcaatca caaaaccgtg tttatgcgca agacctcgga acaaatagca acagttaaaa
     5161 tgtcgcgctt cgacgtccat tcattcgccg caatccgcct taaaaagcac gccgttcggc
     5221 cgcaatgagc gccactctca agacgcagta cgacgcgctc gtcgcggccg gccggctcga
     5281 agccgatccc gcgcaaagcg ccgtgatcga agaattggcc gcgcttgccg aaaggctgga
     5341 gcgttacgcg ccggcgcggc ggccgaacgg attggcccgg ctcatcggcg cccggccggc
     5401 cgagccgccg cgcggcctct acctctatgg cccggtcggc cgcggcaaga cgctgctgat
     5461 ggacctcttc ttcgaggcgg cgccgaccgg cgccaagcgc cgcgtccact tccacgcttt
     5521 catggccgat gttcaccagc gcatccatga atggcggcag cgccacaagg ccggccacgc
     5581 gacgggggac gatccgatcg cgccggtcgc cgccgacctt gccgccgaag cgtggctcct
     5641 ctgcttcgac gagttcagcg tcaacgacat cgctgatgcg atgattctcg gccgcctgtt
     5701 cgcggcgctt ttcgccgcgg gggtcgtcgt cgtcgcgacc tcaaatgtcg cgccggacga
     5761 cctctacaag gacggcctca accgcgcgct gttcctgccc ttcatcgcct tgctgcgcga
     5821 gcgcctcgac atttgcgagt tgcagggccg cgccgattac cggctcgaga agctcgtccg
     5881 cgcgccggtc tactaccatc cggccgacgc cgcggccaag gccgcgctcg gccgcgcctt
     5941 cgagaggttg accggccatg cgcgcggcgc gccctcgaca atcgagctgc tcggccgctc
     6001 gctgtccatc cccgaggccg atgacggcgt cgcccgcttc ggctatgccg atctctgccg
     6061 ccagccgctc ggcgcgacgg atttcctggc gatcgcccgg cgattccata cgatcattat
     6121 cgatgacatc ccgatcctcg atccggagcg gcgggacgag gtcaagcgct tcatcaatct
     6181 cgtcgacacg ctctatgacg agcgcgtcaa gcttgtcgcc tcggcggcgg ccgagccggc
     6241 cgatttgtat tcgggcggcg acgggttcga agccttcgaa ttcgcccgcg ccgcctcccg
     6301 cctcgtcgag atgcgctcgg ccgattatct ggcgctggcg catggccatg ccggcgcctc
     6361 ggtctccggc gatctcggcg ggctggtcga gacctgaacg cgatctggat cggcgcggcg
     6421 tcttatgcga gcgtggcggc gtcggggggc gaaacagtgc cgcgatcgca atcagccgag
     6481 ccggtcgagg cgcgcgtcct ggagatcgcc tgcgcgcatg tgcgcaaatt cggggccggc
     6541 cgcacgacga tcgtcggcgt cgcgcgcgag gcgggcatga cccacgccaa tgtctaccgc
     6601 tactttccct cgaaagaggc cctgttcgaa gagatgaccg ccgcctggtt gcggccgctg
     6661 gagacgcgct tgcgcgaagc cgccgacggg gccgatcccg cctatgacaa gctcgagcga
     6721 atgctgatcg ccatccaccg cgcctatcgg caaaagctgg aaagcgacgc gccgcttttc
     6781 gacctgctcg tcgaggcgct gcagaaaggc aaaagcagcg cccgcaagca tcgcgcccgc
     6841 gtgcaggccg atttgcagcg cgtcgtcgag gaaggcatcg ccgccggggc cttcgcgatg
     6901 accgaccggc gacgcgccct gtcgctggtc ttcgacgccg cgcaccgctt cattcatccg
     6961 ctcgccgtgc ggctcgacgc cgaaaccccc gcggcggcgc tggaggcgcg gttcgagagg
     7021 atcattgcgc tggcgctgag ggcgctgcga tcgggccggg tgtgataata tacgtacatt
     7081 cgatgagacc cccttctccc cgctgcgcgg ggcgaggggt tcgtacgctg gatcgtgacg
     7141 catgccccgt tacaagctga cgctcgaata tgacggcgcg ccgtttgtcg gctggcagcg
     7201 gcaggccaac gggctctccg tccagcaggc gttggaggag gcgatcctgg cggcgagcgg
     7261 cgaggaggtc gtcgcgaatg gcgccgggcg caccgacgcc ggcgtgcatg cgaccggcca
     7321 ggtcgcgcat gtcgatcttg ccaaggattg gcgtggtttc cggctgagcg aagcgctgaa
     7381 cgcgctgctc gtccccaagc cgatcgccgt tcttgccgcc gagcgggtcg ccgacgattt
     7441 cgacgcccgc ttctcggcgc gcatgcgccg ctacctttat cgcatcgtca atcgccgcgc
     7501 gccgctggcg ctggagcgcg agcgcgcctg gcgcgtcaag cgcccgctcg acgccgagaa
     7561 gatgcgcgaa ggcgcggcgc tgctcgtcgg caagcacgat ttctcgacct tccgcgattc
     7621 gcaatgccag gcggcgagcc cggtcaagac attgtccagt ttcgatatcg agcgtatcgg
     7681 cgacgagatt gtcatgcggc ttagcgcgcg ctccttcctg catcgccagg tgcgctcgat
     7741 ggtcggctcg ctcgagcatg tcggctcggg caaatggagc gtcggcgatt tgcgcgccgc
     7801 gctcgaagcg cgcgaccgcg cccgctgcgg ccaagtcgcg ccggcggcag ggctgtacct
     7861 ggcgaaggtg gagtactgag gggccggcta tcctcccccg cgaaagcggg ggagggggac
     7921 catgcgaagc atggtggagg gggcggccgg cggttgacga taatctcctg acgccgaggc
     7981 cccctccacc gcgctgacgc gcggtccccc tcccccgttg cgcttcgctt cacgggggag
     8041 gatcaggtct cgctcaaaat ccggcgaagt acttttcgag aatccgctcg tagatttgcg
     8101 acaaagcctc gatgtcgtcg actggggcgc gctcgtcgac gccgtgcatg gtctcgccga
     8161 ccaggccaag ctcgaccacc gggcaggcgt tcttgatgaa gcgcgcgtcc gacgtgccgc
     8221 cgctggtcga gagggtcggc cgccgcccga ccacgtcttg cacggcgtcg gcgacgaggc
     8281 cggtgaaggc gccgctcggc gtcaggaagg cggtggcgtt ggtcggatcg aaggtcaatt
     8341 cgtagcgcat gcggccggcg gcgcgcgcga cgcgcttctc gatttcggcg cgcaacgtct
     8401 caggcgtcca gatgtcgttg aagcggatgt tgaagacgat ccgcgcctcg ccgggaatga
     8461 cattggccgc gcgattgccg acatcgacgc tggtcgcctc gagattcgac gcgtcgaaag
     8521 ccggcgtgcc ggaatcgagc ggcggctcgg tgagcgcggc gaggactggc gcgagcgcgc
     8581 gcaacggatt gtcggcgaga tgcggatagg cgacatggcc ctgcttgccg atcagggtca
     8641 ggcggccggt cagcgagccg cgccttccgt gcttgatcat gtcgccgagc ttctcgcggc
     8701 tggtcggctc gccgagaatg caatgatcga agcgctcgcc cttggccaag gcccagtcga
     8761 ggagcttgac cgtgccgtta accgcggcgc cttcctcgtc gcctgtaatc agaaagccga
     8821 ccgagccctt gacgccctcc tttcggacga cgaagcgcaa tgcggccgct accgcggcgg
     8881 cgacgccgcc cttcatgtcg caggcgccgc ggccccagag cattccgtcg gcgatttcgc
     8941 cggagaacgg atcgaagcgc catttggcgg gatcgcccgg cggcacgacg tcggtatggc
     9001 cggcaaagac gagatgcggc gcgccgcggc cgaagcgggc gtagagattg tcgacccttg
     9061 ctgcgcccgg cgcgtcgaaa gagacgatct cggcggcgaa gccggcctcg accaggattc
     9121 cgcgcagata ggtcagcgcg cccgcttcgt cgggcgtcac cgaggggcag gcgatcagcg
     9181 cgcgggcgat gtcgagggcg gcgaggggcg gctgcggcat gacgagcgaa tcagtctccg
     9241 agaggttcgt tggctcgcga cgtacgtacg gcccgcgcgg cttaccctca tccggccgct
     9301 tcgcggccac cttctcccgc aagcgggaga aggggttgtc gcgcgccgat gaagcgctag
     9361 gcgcgccccc tctcccgcaa gcgggagagg gccggggtga gggtaatcca gaggacggat
     9421 agaaactacc tatggctctt taacgacaca ttcgcctcac ctgggccgca tcgcgctgac
     9481 ctgcgcggcc cagtcgagcg cgacatcata gatcggcggc tcgacattgg agagcagcgt
     9541 ccacaagccg gcgatctggc ttcgcttaag cgccttgatc agcacgcggc cgtcggacag
     9601 gccgcagacg cacatatggc cgacaagatc gtcgccgggc ggatcgcgca cgtcgtcgta
     9661 gaagaccagc caattgtcga acagcgcgcc gagcgaatgg ccgcggacct gcaccgcgac
     9721 cgtttgcgag ctcgcgccgt ccggcgccgg gacttcgtcg aacgggccct ggccctcggc
     9781 gtagaaatgc gcctcggcgc cggcgccgac aaagccgacg accggaacga gaaggccgga
     9841 gggcggccgc cgcccgccgc cgaggaaagg cggcggctcg acgccgagat aattggcggc
     9901 gacgacaatc tcctcggccc tgagccgcct cgcgccattg agcaggtcgg tgaccgccga
     9961 cggcgatcgg cctagcgcct tggcgaggcc gctccgggtc tttccggtcg ccagaagggc
    10021 tttgcggatc caatcgacga catcgttcgg catagccgaa ttattctgct tttcagaacg
    10081 aaaacaatta tgatttccag aaaatttcct tgacgggata ttctgatttt cagaatatag
    10141 ctcgccgctg aacaacgcac tcgcccaggt cgaaggagcc gctcgaaaat ggggaaagtc
    10201 cgcctgcgcc gccgcgcgcc caaaatcgac accgcccgga tggaggcgat cgtcgcctgc
    10261 gcggagcatt taagggattt gaagcgcgtt catggcgcgc cgccgccgga catcgaggtc
    10321 gcgacaagcg ggacgccgca gcgcctcgcg ccgatggcga cgaactccta ttgcacgtcg
    10381 cctgccgaat tatgcgcgga gctcgtcaag tgagcggccg cgcctccacc cgccgtccga
    10441 cccgttcgcg ccgcgatgcg atcgccgcgc cggagcggct cgaagcgacg cccgagcgtc
    10501 tcgcccgcgc ccgcgactgc ggccaggaaa tcgctctgga ccgcgagcgc cggacgcgga
    10561 tcgacgatcc gttcgacgcg atgcgcgccc gtcgcgcgct ggcgccgcag gattcgcggc
    10621 tcaacgactt gcgctggctg atcggcgaag cgctgcgccg cctgcatcac cgagccggcc
    10681 tcgattcctt gcgcgccatg gctcccgacc gcgtcggcct ggtcgcgcct ggcccccgcc
    10741 cgggcctgcc gctctcggac ctcgctttgc atgcgcggga caagctgcgc gcctgcgaaa
    10801 gcctcgccgg cgcggcggcc tggccggtcc tgcgccggat tgtcatcgaa ggggccaact
    10861 tgcgcgattg caggccgctg atcgccgagg tctccacccc ttggcgggcc gacgccatcc
    10921 tcgccgaccg cctgcgctgc ggcctcgatg cgcttggcgg ccatctcggc gtcacggcga
    10981 aaagatcagg gtttgcgtcc ggttagaggc aaaaagcggc gcatccggtt cactggccgt
    11041 tcacatggag catgcttagg tcggcgggcg ttggagagac ctcgaggagt ttcgaaaatg
    11101 acacgctctt tagcaagcaa agcgctggcc gccgccgcag gcgcctcgct cttcgccttg
    11161 actttggctg cgccggtccc tgcttcggct ttcacgttgg ccgcgccgga tctcggtcat
    11221 tcctttgccg ataacggtca agtgcacaag gcctggtggg accgctgggg ccgctggcac
    11281 ccgaattatt ggggtccgcg ctacggctgg cggcccggct ggggctatgg cggctggggt
    11341 ccgcaccgcc attgctggat gagcccgtgg ggctggcgct gccgctggta ttgagcgcct
    11401 cggcttgaaa atgaacgctg cgggggatgc gctcccccgc ggttttcagg atgtcactcc
    11461 ccgcggaaag cgggaatcca gagcgcctca gcgtttgtag gagggtgccg tactggattc
    11521 ccgcttgcac gggaatgaca ggctctagcc ctgcatcttg gcgatgagcg cgtccgacac
    11581 ttcgaaattg gcgtagacgt tctgaacgtc gtcattctct tcgagcgcgt cgaccagctt
    11641 caaaatcttc tcgcccgcct cgtcgtcgac ggcgatcgag ttttgcggcc gccagacgat
    11701 cgcggctttt tgcggctcgc cgaattgcgc ctcgagcgcc ttttgcgatt cgcgcagcga
    11761 ctcgatcgag gtgacgattt catgcgtctc gccgctcgaa gcgacatcgt cggcgccggc
    11821 ttcgatcgcc gcctccagca tggcgtcgtc ggaggcgact ttccggtcgt agacgatgag
    11881 gccgacgcga tcgaacatga aggagacggc gcccgtttcg gcgagcgagc cgccggcttt
    11941 ggcgaagctc gaccgcactt cgccggcggt gcggttgcgg ttatccgtta gcgcctcgac
    12001 gatgatggcg acaccgcccg gcgcatagcc ctcatagcgc acctcgtcgt aagtctcggc
    12061 gtcgccgccg gcggccttct tgatcgcgcg ctcgatattg tccttcggca tgttctcggc
    12121 ccgcgccgcg atgacggcgg cgcgcaggcg cggattcatc gccggatcgg gcaggccgag
    12181 cttggccgcg accgtgatct cgcgcgccag cttggagaag agcttggagc ggatcgcgtc
    12241 ctgcttgccc ttcttgtgca tgatgttttt gaactggctg tgaccggcca ttttcccgtc
    12301 tctcgcaatc agtttggctc agcgcgctgg cgcgtcattt cgccaaggga atagtccgtt
    12361 tcatgaaccc gtcgaagagc gggaccgtga aagcgttgtc gccatgagca gggctataga
    12421 gcatcccctt ggccacaaga gagctgcgca ccggcgcgac attggtgact ttttttccaa
    12481 gcacttccgc aatatctccc gaacggtggg gaccggatcc caactccgcc atggctcgca
    12541 tgtatcgctt ttccgccggc gtcaagcggt cgaagcggac gcgaaaaaag ccggcgtcaa
    12601 gatcggccaa ggcgctttcg gtcgcttttt ccgcgtcgac cgtctcgatg ggggaaactt
    12661 tcgcgatatt ccacgcgtgc ttgccccatt cctgtagaaa atatggatag ccgtgcgtac
    12721 ggcgcaatat ttcgacaatc gctccgggcg aaaattcgac cccctcgcgt tcggccggct
    12781 tgcacaaggc caatgtggcg tcgtcttggc cgagagcccc tatttgcgcg aattcgaaaa
    12841 ggcgctcggc ataggacttc gccctgccgg ccaagcccat caattgcggc aagccggcgg
    12901 cgatcatggt gacgggaagc tgttcttgag aagcgcggtg caaggccgca atcagcgccg
    12961 atagctgggt ttccgggacg tactggagct cgtcgattgc taaaacgaga gcagtctggc
    13021 gctctctcgc cgccgagccg acagcgacaa agaggtcgga aagatcgcgc tcaaaatcgc
    13081 cgctgtcggc gatgcccatc tccgatattg cgtcaaaatt gaactccacg tcctggaatt
    13141 tgattttgaa cgccttcgcg gcgctggcga gaacgcgccc ggcaaaatcc aatctcgctc
    13201 taagtgcttc aattctgctc aattgcagca gtccgatccg cagagccgga acgagaaggc
    13261 ttggcaggga ccgatcttcg ggcgtctcga tcgaaacgcc gataattcct ctcgcttccg
    13321 catcgcggcg gatgtggttc aaaagtacgg tcttgccgac gccgcgcaga ccgaccagga
    13381 tcaaactgcg cgcagggcgg cccatccgga tgcgatccag cgcgatggcg gcgcgctcga
    13441 tcgtgtcgtc gcggcctgcc aattcggggg gcggactgcc tgcgcccggc gcataagggt
    13501 ttgttcgcgg gtccaatttt gaggcttctt atgaaagtta tgcaaaaaag ataaattact
    13561 ctaaactcac cataatttca aactaccaga aatccggggt cgccggcgtg agaatcccgc
    13621 cgatcctcac cgcggcgatc tttctggcca gtccaccctc gccggtctcg acggcgacgc
    13681 cgcagagcgt cgccgggcca agggccggct cgaagcgcgc cgagggcagg cggcgggtaa
    13741 agcggcgcag cggctcctcc ttatccatgc cgatcaccga atcgtagtcg ccgctcatcc
    13801 cggcgtcggt gatgaagccg gcgccgccgg gcaggatgcg atggtccgcg ctcggcgtat
    13861 gggtatgggt gccgacgacg aggctgacgc ggccgtcgac atagaggccg aaagcctgtt
    13921 tctcgctcgt cgcctcggca tggaaatcga cgatcgccgc atcgcatccg gcgccgaggg
    13981 gacaggcggc gagctcctgg tcgatgcgcg cgaaaggatc gtcgagcgca tccatgaagg
    14041 tgcggcccat catgttgacg accagcatct tgcgcccgtc cttggcctcg atgagattgg
    14101 cgccgcggcc gggcgtgccg ggcggataat tggccggccg gatcagccgc ggctggcgct
    14161 cgatatagat gagcgcctcg cgctggtcga aagcgtggtt gccgagcgtc acagcgtcgg
    14221 cgccggaatc gatcagctcc tggcagatcg cctcggtgac gccgaagccg ccagccgcgt
    14281 tctcggcgtt gacgacgacg aaatcgagcc gccatttctc gcgcaagaga ggcaattgct
    14341 ggttgacgat ggttctgccg cttcgcccga ccacgtcgcc gatgaataag agacgcatgc
    14401 cgggctacga atccctgcaa tcgatcaatt cgctctccgt cagaacgtag tcgagccgct
    14461 cgtcatgcgg ctcgtcgggc agaatagggc attccgcgac gctatagccg accccgacgg
    14521 cgacggcgcg gcctttggcg cgcaaggccc ttaaggtcag atcgtaatag ccggcgccga
    14581 agccgatgcg atggccgcgg cgatcgaaac aagcgagcgg cacgaagagc aggtccggat
    14641 cgcgcgccgg cgcgtcttcg agcggctcgg gaatgttcat ccgcccgggc cgggtcggct
    14701 cgcccggccg ccagcggcga aagacgagcg gcgcgccgcg cgttccggtg atcggcagca
    14761 gcgtcacgaa gtccgcttcc gcgagcgcgg cgagcagcgg gccggtatcg ggctcgtctg
    14821 gaaaggcgtg aaacgccgag accgcccgcg cgccggtgcg cctcgcgagg cgcaggccct
    14881 cgcgggcgag ccgcgccgag aatttcgctc tgtcggcttg gccgagggcg cggcgccggg
    14941 caagcgcggc ctcccgcaag tccgccttgg ccgcggctgg ggccgtcgtc accggctcat
    15001 tgctccctgg aagcggatat gcgcggggcc gcgatggccg tgggacggcg atcccgggaa
    15061 cctacaaagt aggtgggcgc cgttgtgacc aagcccacgg gcaaggccag ggacagctcc
    15121 cttcaggatc gtaaggcccc ggggataagt gtcctcacgc accccgcagt cccgctgagc
    15181 taatgtagga cgcaaggagg cgattggcta gccggcccgc gctcaagccg ccatggcgaa
    15241 gcgcacggcg aggccggtcg cgacgacgcg gcgcagcgcg acgccgtcaa tatcggcgcc
    15301 catgacgccg tccatcttga attcgagccc gacgatcgcg tcggcgccgc attcctccgc
    15361 ttcgcggatc agctttctca acgccgcctg ccggtccgct tcgccgcccg ccgcattggc
    15421 ggcgcgccaa gccccggccg ccttgatccg gccgatcgca aaggtctggc ggccgtcgtc
    15481 caattgctgg ctttcactga tctgcatgac gctgattgag gccctgttgc tcgtggcgtc
    15541 ttccataaag agcggctgtt tacgcgaagt ttaacggcga ctcgccgcgc cggcttcgcg
    15601 cttgcctttc gaggacatgg tgaacggctc ctgagagaaa cgttcgagga aacccctttc
    15661 gcgattacgg gacgccataa agcaaaggcg gcgccggaag gcgccgcctt gagaggtcga
    15721 gagcgccgcc gctcaggccg attggctggc ggactcggcg gcctgctgct tttcgagatc
    15781 agcgccggtc tgctgatcca cgacgcgcat cgacaggcgg atcttgccgc gatcgtcgaa
    15841 gccgagcagc ttgaccttga ccttatcgcc ttccttgacg acgtcggtcg tcttggcgac
    15901 gcggcccttg gcgagctgcg agatgtggac gaggccgtcg cgcgagccga agaagttcac
    15961 gaaagcccca aaatccaccg tcttcaccac ggtgccgtca tagatcatgc cgacttccgg
    16021 atcggaggcg atcgacttga tccagttgat cgccgccttg atcttggcgc cgtcggcgga
    16081 ggcgatcttc accgtgccgt cgtcctcgat attgatcttg gcgccggtct tttcgacgat
    16141 ctcgcggatg accttgccgc cggagccgat cacttcgcgg atcttgtcgg tcgggatttt
    16201 tatcgtctcg atgcgcggcg cgaattcgcc gagttcggcg cgcgagccgg acaaggcttt
    16261 ggccatctcg ccgagaatat gcaggcgccc gtccttggcc tgcgccaggg cgaccttcat
    16321 gatctcctcg gtgatgccgg cgatcttgat gtccatctgc agcgaggtga cgccttccgt
    16381 ggtgcccgcc accttgaagt ccatgtcgcc gagatgatcc tcgtcgccga gaatgtcgga
    16441 gaggacggcg aagcgcttgt cctcgaggat caggcccatg gcgatgccgg cggtcggccg
    16501 cttcatcggc acgccggcgt ccatcagggc gagcgaggag ccgcagacgg tcgccatcga
    16561 ggacgagccg ttcgactcgg tgatctcgga aacgacgcgg atcgtatagg gaaattccgc
    16621 cgcggtcggc agcatcggat ggatggcgcg ccaggcgagc ttgccatggc cgatttcgcg
    16681 gcggccaggc gagcccatgc ggccggtctc gccgaccgaa tagggcggga aattgtaatg
    16741 gagcatgaag cgctccttat acgttccctc cagactgtcg acgtactgtt cgtcctcgcc
    16801 ggtgccgagc gtcgcgacgg cgagagcttg cgtttcgccg cgcgtgaaca aagccgagcc
    16861 atgggtgcgc ggcaacacgc cgacttgcgc caggatggga cgaacggtcg tcagatcgcg
    16921 gccgtcgatg cggcgcttct ggtcgagaat gttccagcgc acgactttgg cctgcgcctc
    16981 gtggaacgct tcggcgactt cctccttgga aaatttcgct tcgccctcgt ccggcgcgag
    17041 cgccgccatc accttggcct tgaccgcgtc gaccgcggcg tagcgctcct gcttggcggt
    17101 gatcgcgtat gcggcgcgca gctcgctttc gccatgttcg agcaccgcgt tcagcaccgc
    17161 cgacttgtcc ggaacgacga gctcgcgcgg ctctttggcc gccttttcgg cgaggcggat
    17221 gatcgcctcg atcaccggtt ggaaggcgcg atgaccgtcc atcaccgcct gcagcatcgt
    17281 ctcttcggaa agctccttgg cttccgattc gaccatcagc accgcttccg ccgtgccggc
    17341 gacaacgagg tcgagggccg attctttcat ctcgtcgatc gtcggattga ccttgagctg
    17401 gccgccgaca tggccgaccc gcgccgcgcc gatcggcccc atgaagggaa tgccggaaag
    17461 ggtcagcgcc gccgaggcgg cgaccaacgc caaaacgtcg ggatcgtttt cgaggtcatg
    17521 gctgaggacg gtgacgatga cctgcgtgtc gcagcggaag ccatcgggaa agagcggccg
    17581 gatcggccgg tcgatcaggc gcgaggttag ggtctcgcgg tcggacggac gcccttcgcg
    17641 cttgagatag ccgccgggaa tgcgtcccgc agcgaaggct ttctcctgat aattgacggt
    17701 caaggggaag aaatcgacgc ccggcttcgg cgctttggcg gcgacgacgg tggcgagaag
    17761 gctggtctcg ccccaactcg cgaacaccgc gccgtccgcc tgtcgggcca tccggccggt
    17821 ttcgagcgtg agcttgcggc cgcaccaatt gagctcttca cgttggatat cgaacatggt
    17881 cgttcatctt tcggtcatgg cgaaagcatt aacggccatg cgcaagacgg cgagaggctg
    17941 gccgcgaaac cgctcgaagc ggttggcgcc agcccctggc gatcctgcca tggcgcgtcg
    18001 ctttcaaggt ctaattgtca aggaaggcgg ccccatgccg ccgtcccctt tctcgttgtc
    18061 aaacaatcgt cagcgacgaa tgccgagccg ctcgagcagc gacttgtaac gggcttcgtc
    18121 ctgccgcttg acgtaatcaa ggagcgttcg ccgctgcgac accatcttga ggaggccgcg
    18181 gcgcgaatgc gtgtccttca catgcgtttt gaaatgttca gtgagattgg cgattctttc
    18241 agtgaggatc gcgatctgca cctccggcga acccgtatcg ccggccttca cagcatattc
    18301 cttcacgagc gcttgcttgc gctcgacgct gatcgacatc gggtctttcc tttctcgttg
    18361 aagccggcgc cgcgctcttt tcgagcgatc ggcgccctga ggcgttcggc gcatcggcgc
    18421 ccgccggatc ggtggccgca ccgggatgtc gtccaggacg gtcacgttga gcgaggcgaa
    18481 aatcgcctcg cgggcgctct tatacaggaa agaggcggtt gcgccagcct ctttcggccg
    18541 ggcgttccgg cttcgaaggc cgtctagaga cccgtcgcaa aggccgcgag ggagagaaga
    18601 gcgaccgcgc cccacgcgac ggcgctggcg aggccgggcg gataagcctc gcgccattgc
    18661 ggcaagcgcg cccagaaggc gtaacttgcg atcggcagcg gcaacaggac gaggaggacc
    18721 gcgccgaggc cgactcgccg cgacgcagcg tcgagcgcga caaaggcggc gaggccggag
    18781 atcggatgca gcagcagcgc ggcgagcgcc gcccagaccg gcatccggcc tttcgccgcc
    18841 ccgacggcga gcatcagcgc cagcacgctc cataacccga gcgtcagcaa gaagccggcc
    18901 caagcctggc tataggcctc ctcgcccccg gccggcgggg cgccgctatt ggcgagcaga
    18961 gccccataga ggacgatcgc gaccgccaag agcgacatca tgccgagcgg cgcggcgtgt
    19021 agaattttaa gccagtcgcg cacggaatcc cccttgccga acgtcgccgg cgccgctgga
    19081 gcgcttacgg gacgcgcgaa ttgtcgcgat cgttttgaat cttgccgcca tcgccgcaac
    19141 cgcgccggct ttcacgcgcg gccgttttca ggtggaatga caaattgctg aaacgccgaa
    19201 gaatcgcacg tttgcgcctc gcctcgcggc ggtataagcg atccgccaag cccgattgcg
    19261 gctcttcgcc ccattttttg cttcaagaga ggaatcctga tgacgcggct cgatgataaa
    19321 ctggctcgca tccgcgacgg caaatatcgg cgcgccgatt tcatcatcgc cgacgccaag
    19381 gaccccgata tggggccaag catggccgcg gccggcccgg cccggcaaaa ggacggcgcc
    19441 gcgacgcgca accggacgcg cgaggaattc ctcggccaga tcagggcgat tgtcgagcaa
    19501 gacgtcgtcg atatcatgct gacgtccgcc tccaatctcg atcgcctggc gcgcagcggc
    19561 gtgttcaaag ggagcaagat ccagccggcc atccgcgcca acgacacgac cgacatttgg
    19621 cgggtgcgcg gcggcgccta tccgcaaaag ccgtcgcggc cgttccgctc ggccaatctc
    19681 tgccgggtga tgtggggctc ggcgacgccg aaagtcggct cgccgccgac cgggaccgat
    19741 ctcggcctct actcgatcac gttcaacaac gacatcgacg ccgatatcgc gacgctcgaa
    19801 gccttcgccg aattccgcgc cgaagcggct gcgctcggct tccgttattt cctcgaagtg
    19861 ttcaatccga atgtcgcgac cggcattgcg ccggaaatcc tgccgcatta cgtcaacgac
    19921 tgcatccagc gctgcctcgc cggcctgacc gacgccgagc ggccgctgtt cctaaaagtg
    19981 gtctataacg gccccaaggc gctggaggag atggcgtcct acgatccgag cctggtcgtc
    20041 ggcgtgctcg gcggcggcgc cggcacgacg cgcgattgtt tcgaactgat cgcccaggcc
    20101 gagcgctacg gcgcgcgcgt cgcattgttc ggccgaaaga tcaatctcgc cgaatcgccg
    20161 ctcgccatgg tcacccatat gcgcgccgtc gccgacggcg cgatgagccc gagcgaagcg
    20221 gtgaaggcct atcacggggc gctgcagaaa gcggggatca ggccggaccg cgatctcgcc
    20281 agcgacgatc tgatcaccga agccgctttg aagacgggct aagccgtggc ggcgcggcgc
    20341 ggcttcgtca cggcgggcac atggtgcgtc gataacaaca agctgatcgc cgcctggccg
    20401 gaagaggata cggcgagcga agtcctcgcg atcgaccgcc agggcggcgg ttcggcctgc
    20461 aacctggcga tcgacatgaa gcggctcgat ccgggggcgc cggtcgagac gatcggcctg
    20521 atcggcgacg acgagaacgg ccgcttcctc gccgcgcaat gcgacgcgca tggaatcggc
    20581 cgcgcccaga tgcgcgtcgt tcctggcgag gcgaccttct cggtcgacgc gttcacggtg
    20641 gcgaagggca aaaggacgca tctcttctat ccgggcgtcg ccaagcggct gagcccggac
    20701 gatttcgatg tctcgaaaac tgacgcgcgc attctgcatc tcggcctgcc cggcgcgcac
    20761 caggtcatgg acgcgccctg gcgcgacgag gcgaatggct gggtcgcggt gctgaaggcg
    20821 gcgcggagaa acggccttga gaccaatctc gaattgatga cgatcgcgcc ggagcggctg
    20881 atcgctctag cgcggccctg cctgccgcat ctggattatc tgatcgtcaa tgatttcgag
    20941 attggcgcgg cgacggggat tgaaacgcgc gtcgggggac gtaccgactt cgccgccgtg
    21001 cggcgcgcca tcggcgccgc cctggcctcc gggccgatgc gcgtcgtcgc cgcgcatttt
    21061 ccggaaggcg cgatcgccgg cgcgcgcgac ggcgccacgg ccgcaatcgg ctcgctcgcc
    21121 atgcccgaag ccgacatcgt cggcgccaat ggcgcgggcg acgccttcgc ggcagggttg
    21181 ctctatgggc tgcatgaggg ctgggatttg cggcgcgcgc tggcgctggc ccattgcgcc
    21241 gcggcggcct cgatgcggaa gatttccacc accgaaggag tcgggacctg gcgcgattgc
    21301 ctggcgctcg gcgagcgctg gggattccgc gacgcgccgg gatagcgtac cgccgccgat
    21361 acagtagacg cctcggcggg cgcctttggc gctcgcttcc aagaaagaaa tttgaatttc
    21421 aaaatttctt tttggcggaa tcaatgaaat caatgcccga aaatttgcat ctccaaattt
    21481 ttccaaactt ttatttggcg gttttgcgga ctatcagaga cttacgaagc gaaaaatttg
    21541 gatccgcctt tttggttcgg atcgtccctc ctgcgccgta ccttgcaagc gcaaaccgcg
    21601 tcctgcggcc tgctgcgcaa atagtttgag tcggaaatgg cgagatgtcc agaaaatgtt
    21661 ctgatcgacc gcgtggaagg ccgcgtacgt cgctgaagcg attgcgaaaa atcgttcacg
    21721 tccggt
//
LOCUS       DATGJW010000006         6540 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1038348, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000006 DATGJW010000000
VERSION     DATGJW010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6540)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6540)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6540
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038348"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            184..1029
                     /locus_tag="VKU03_00205"
     CDS             184..1029
                     /locus_tag="VKU03_00205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HLJ69707.1"
                     /translation="MTSLATDPARAHAVRPRIVYRHSAIVRVTHWINALCFALLLMSG
                     LQIFNAHPALYWGQASTFDRPFLSLGAEEDANGGVKGVTTVLGHSFNTTGILGASRDD
                     DGEMAARGFPTWITIPSYQDLATGRRWHFFFAWILALNGLVYFGNLVTKRRMTRDFAL
                     SLAELRSIPHAIVEHARLRFPKGDAALHYNVLQKLAYLSVIIAFPVLVLAGLTMSPGV
                     DSGFPWLLELFGGRQSARTIHFLLASYLVLFLLVHLVMVLVSGVFNNLRSMITGRYRI
                     EEEKP"
     gene            1026..1808
                     /locus_tag="VKU03_00210"
     CDS             1026..1808
                     /locus_tag="VKU03_00210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004119459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLJ69708.1"
                     /translation="MRRDVQPTRRRLLAALTAGAAFGLSGCDRLSQSPSWLSLLDSAE
                     GLTRRAQRLLIGSRQALAPEYTEADISKVFKPNGSVDPDDPDYVTAKQENFANWRLAV
                     DGLVEKPLSLSLAELRQLPARTQITRHDCVEGWSCIGKWTGTPLKELLDRAGLKPNAR
                     YIVFHCADTLEQTLDGTGRYYESIDLVDAFHPQTILAYDMNGASLPVEHGAPLRLRVE
                     RQLGYKMAKYVMRVEAREEFASLGRGRGGFWEDRGYEWYAGI"
     gene            1960..2580
                     /locus_tag="VKU03_00215"
     CDS             1960..2580
                     /locus_tag="VKU03_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HLJ69709.1"
                     /translation="MTARLHRGDLPADLELGPVVAIDTETLGLDVRRDRLCVVQLSRG
                     DGTAEIVQIAPGQKRAPNLEKLLADPAVLKLFHFGRFDMAALANAFGVMAAPVYCTKI
                     ASKIARTYTDRHGLKDLTRELLGVDLSKVQQSSDWGAPDLSEAQIAYAASDVLHLHAL
                     RDKLDLMLAREGRAELARAAFAYLPDRARLDLAGFAEMDIFAHASP"
     gene            2688..3386
                     /gene="lptC"
                     /locus_tag="VKU03_00220"
     CDS             2688..3386
                     /gene="lptC"
                     /locus_tag="VKU03_00220"
                     /inference="COORDINATES: protein motif:HMM:NF018537.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter periplasmic protein
                     LptC"
                     /protein_id="HLJ69710.1"
                     /translation="MNYAVAIEPSDRPYAGERAAAFAAARRHSKLVRFLRWAILGGAA
                     GAVAILALIAIFDPLGRITGDASFAGLSLDGTRVAMDRPKLAGFRTDGRPYLVNAKRA
                     VQDVLHPTIVELHGIDAEVGISGNGASKVTADLGVYDSATEHMDLSHNVSVKSAEYDV
                     RLQSASIDFKAGIYRSRDPVTVVTSNGATINADSVAAIDNGRELIFEGHVRSVLQVGD
                     AAQRTSAELKGTDQ"
     gene            3533..4144
                     /locus_tag="VKU03_00225"
     CDS             3533..4144
                     /locus_tag="VKU03_00225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LptA/OstA family protein"
                     /protein_id="HLJ69711.1"
                     /translation="MSTAIADPATAASKSASASAILPGSNSKEPISIDADKLVYYDKE
                     QKAIYSGNVVAIQGDSKLTCSALTIFLARNEAPNGASKDGAGAAASGEGPGASTSQVK
                     RMEASGPVTVVSKTQVATGDRGVYDKTENKVWLFGNVTLSDGGNVTKGDKLTYDLTTG
                     QAVVETGHSSQRVHGLFIPNSDEGAEKPGPGKKRLPDGAAPKP"
     gene            4188..5075
                     /gene="lptB"
                     /locus_tag="VKU03_00230"
     CDS             4188..5075
                     /gene="lptB"
                     /locus_tag="VKU03_00230"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012172446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter ATP-binding protein"
                     /protein_id="HLJ69712.1"
                     /translation="MNDDFASSGAKPRAFGGRWIGWGVTGSGGGAALAPAGPYAGFGE
                     SQPADYGRSAETGALAVYNLGKSYSGRAVVKDVSISVRRGEAVGLLGPNGAGKTTVFY
                     MITGLVRPDKGVIELDGHDVTALPMYQRARLGIGYLPQEASIFRGLNVEDNIRAVLEV
                     TEPNRKTREAMLDSLLEEFDIARLRKSPSIALSGGERRRCEIARALAGKPSFMLLDEP
                     FAGIDPIAVGDIQDLVRHLKGRGIGVLITDHNVRETLGLIDRAYIIHSGHVLTEGGPD
                     EIIAHPDVRRFYLGEDFRM"
     gene            complement(5307..5642)
                     /locus_tag="VKU03_00235"
     CDS             complement(5307..5642)
                     /locus_tag="VKU03_00235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HPr family phosphocarrier protein"
                     /protein_id="HLJ69713.1"
                     /translation="MSEAEAAATGEEDCPPPPEFAGALAREIRIVNRKGLHARATAKF
                     VQCVDRFDAEVRVTRCGETVGGDSIMGILTLGAAMGSTITVSASGAEAEAALDAIEKL
                     VASRFGEDE"
     gene            complement(5639..6040)
                     /locus_tag="VKU03_00240"
     CDS             complement(5639..6040)
                     /locus_tag="VKU03_00240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS sugar transporter subunit IIA"
                     /protein_id="HLJ69714.1"
                     /translation="MIGMVLVTHGQLASGFRAALEHVVGPQSQFETIAIGPEDDMEQR
                     RGDILNAVSRVQDGGGVVLLTDMFGGTPSNLAISVMDGGRVEVICGINLPMLIKLASV
                     RETADLEHAVGEARDAGRKYITVASKVLAGK"
     gene            complement(6159..>6540)
                     /locus_tag="VKU03_00245"
     CDS             complement(6159..>6540)
                     /locus_tag="VKU03_00245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="serine/threonine protein kinase"
                     /protein_id="HLJ69715.1"
                     /translation="KSALALALIELAGERGLFGRLIGDDRILVQACNGRLLLRGAPQI
                     PGLIERRGVGLEITPAEPAAVARLAVDLLDDGDAAARLPEAADEVATFGPIRLRRLAF
                     DESSRPFERARAVLAALAAEMTAL"
BASE COUNT         1115 a         2196 c         2063 g         1166 t
ORIGIN      
        1 catgaatcaa ctccgtcggt tgccgcgctt tggtcccagg cgcgtatgca acagatacgg
       61 aagcctcgcc gcgcggtttc aaaaaacggc tggaaatttt cgacgcgccg gcaaccccgc
      121 tttggtgtaa cttttggctc cggcgtccgt aatagcaacg gaggctgaag gagcgctttg
      181 aaaatgactt cgctggcgac cgaccccgcc cgtgcgcatg cggttcgccc gcgcatcgtc
      241 tatcggcatt cggcgatcgt gcgagtcacc cactggatca acgcgctctg cttcgcgctg
      301 ctgctgatga gcggactgca aatcttcaac gcgcatccgg ccctttattg gggccaggcc
      361 tcgacattcg atcggccttt tctctcgctg ggcgccgaag aagacgccaa tggcggcgtg
      421 aaaggcgtca cgaccgtgct cggccattcc ttcaacacga ccggaatcct cggcgcctcg
      481 cgcgacgatg acggcgagat ggcggcgcgc ggctttccga cctggatcac cattccctcc
      541 tatcaagacc tcgccaccgg ccggcgctgg catttcttct tcgcctggat tctggcgctg
      601 aacggcctcg tctatttcgg caatctcgtc accaagcggc gcatgacgcg cgactttgcg
      661 ctgtcgctcg ccgaattgcg ctcgattccg cacgccatcg tcgagcacgc gcggctgcgc
      721 tttccgaaag gcgacgcggc gctccattac aatgtgctgc agaagctcgc ctatctctcc
      781 gtgattatcg cctttccggt cctcgtcctg gccgggctga ccatgtcgcc cggcgtggat
      841 tccgggtttc cgtggctcct ggagcttttc ggcggacggc aatcggcgcg cacgatccat
      901 ttcctgcttg cgtcgtacct tgtccttttc ctcttggttc atctcgtcat ggtgctcgtc
      961 tcgggggtct tcaacaatct gcggtcgatg atcaccggcc gctaccgcat cgaggaggaa
     1021 aagccgtgag gcgcgacgtt cagccgacgc gacgccgctt gcttgcggcc ctgaccgccg
     1081 gcgcggcttt cggcctttcc ggatgcgacc gcctgtcgca atcgccaagc tggctctcgc
     1141 tgctcgactc ggcggaaggc ctgacgcgcc gcgcgcagcg cctgctgatc ggctcgcgcc
     1201 aagccttggc gccggaatat accgaggccg acatctcgaa agtcttcaag ccgaacggct
     1261 ccgtcgaccc ggacgatccc gattatgtca cggcgaagca ggagaatttc gcgaattggc
     1321 ggctcgccgt cgacgggctt gtcgagaagc cgctcagcct gtcgctcgcc gagcttcgcc
     1381 agttgccggc gcggacccag atcacgcggc atgattgcgt cgaaggctgg agctgcatcg
     1441 gcaaatggac cgggacgccg ctgaaagagc tgctcgaccg cgccggcctc aagcccaacg
     1501 cccgctacat cgtcttccac tgcgccgata cgctcgaaca aaccctcgac gggaccggcc
     1561 ggtattacga gagcatcgat ctcgtcgacg ccttccatcc gcagacgatc ctcgcctatg
     1621 acatgaacgg cgcgagcctg ccggtcgagc atggcgcgcc attgcggctc cgggtcgagc
     1681 gccagctcgg ctataagatg gcgaaatatg tcatgcgggt cgaggcgcgc gaggaattcg
     1741 cttcgctcgg ccgaggccgc ggcggcttct gggaggatcg cggctacgaa tggtacgcgg
     1801 ggatttaggc ggcggcccag gcgcaagccc gcccttgctt gacgcgccct gccgcggctg
     1861 ttaatcatgc cgccgctggc gaggcggaaa gatgttttat ttcaatagat tggtacgcgg
     1921 cccgggtttt gcccgcggcc ggcttgcccc gtcttcgcca tgaccgctcg cctgcatcgc
     1981 ggcgatttgc cggcggatct cgagctcggc ccggtcgtcg cgatcgacac cgagacgctc
     2041 ggcctcgacg tgcggcgcga ccggctctgc gtcgtgcaac tctcgcgcgg cgacgggacg
     2101 gccgagatcg tccagatcgc gccgggccag aaacgcgcgc ccaatctcga aaagctcttg
     2161 gccgatcccg ccgtgctgaa gctcttccat ttcgggcgct tcgacatggc cgcgctggcc
     2221 aacgcgttcg gcgtcatggc cgcgcccgtc tattgcacca aaatcgcctc caaaatcgct
     2281 cgcacctata ccgatcgcca tgggttgaag gatctgaccc gcgaattgct gggcgtcgat
     2341 ctgtcaaaag tccagcaatc ctccgattgg ggcgcgccgg atttaagcga agcgcagatc
     2401 gcctacgccg cctccgatgt gctgcatcta cacgccttgc gcgacaagct ggacttgatg
     2461 ctggcgcgag aaggtcgcgc cgagctcgcc cgcgccgctt tcgcctatct gccggaccgc
     2521 gcccggctcg atcttgccgg cttcgcggag atggacattt tcgcccacgc ttcgccttaa
     2581 ccaggccgct ttgcgctgca acttcgccat cccttttctt taacggccgt catattatgt
     2641 gacccgccgg ggcgatcaga gagcgccgcg gcttcggagc gagagttttg aattacgccg
     2701 tcgccatcga gcccagcgat cgcccctatg cgggcgagcg cgcagcggct ttcgccgcgg
     2761 cccggcgtca ctccaagctg gtgagatttc tgcgctgggc gatcctcggc ggggcggcag
     2821 gggcggtcgc gatcctggcg ctgatcgcca tcttcgatcc gctggggcgg ataaccggcg
     2881 acgcctcgtt tgcaggcctg agccttgacg ggactcgcgt ggcgatggac cgcccgaagc
     2941 tcgccgggtt ccgcacggac ggccggccct atctcgtcaa tgcgaaacgg gcggttcagg
     3001 atgtcctgca tccgaccatt gtggaattgc acggcatcga cgccgaggtc ggcatttccg
     3061 gcaacggcgc gtcgaaagtc accgccgatc tcggcgtcta tgacagcgcc accgagcata
     3121 tggatctgtc gcacaatgtc agcgtcaaga gcgccgaata cgacgtccgc ctgcaatcgg
     3181 cgagcatcga tttcaaggcc ggaatttacc gctcgcgcga tccggtgacg gtcgtcacca
     3241 gcaacggcgc gacgatcaac gccgattccg tcgccgcaat cgacaatggc cgggaattga
     3301 ttttcgaagg gcatgtccgg tcggtgctgc aggtcggcga cgccgcccag cgcacgagcg
     3361 cggaactcaa gggaacggac cagtgatcgg cgtggcgctc gctgtcattc cgcgcggcgg
     3421 ctctctggat tcccgctttc gcgggactga caggggcgtt tcgtcgagag gacggccggc
     3481 cgcgcgccgc tggctttcaa tccgcctcgg cttgtttgct gtagccgcga tcttgtcgac
     3541 ggcgatcgcc gatccggcga ccgccgcgtc gaaatcggcg agcgcctcgg cgatcctccc
     3601 aggctcgaat tcgaaggagc cgatctcgat cgacgccgac aagctggtct attacgacaa
     3661 ggagcagaag gcgatctatt cgggcaatgt cgtcgcaatc cagggcgaca gcaagttgac
     3721 ctgctccgcc ttgacgattt tcctggcgcg aaacgaagcg ccgaacggcg cgtcgaaaga
     3781 cggcgcgggc gccgccgctt cgggcgaagg gccgggcgcc tcgaccagcc aggtcaagcg
     3841 catggaggct tccggcccgg tgacggttgt gtcaaagacg caggtcgcga ccggcgaccg
     3901 cggcgtttac gacaagaccg agaacaaggt ctggctgttc ggcaatgtca cgttgagcga
     3961 cggcggcaac gtgaccaagg gcgacaagct cacctacgac ctgacgaccg ggcaagccgt
     4021 ggtcgaaacc ggccattcgt cgcagcgcgt ccatggcctc ttcattccga attccgacga
     4081 gggcgccgag aagcctgggc caggaaagaa aaggcttccc gacggcgccg cgccgaagcc
     4141 ttaaatcaag agcggttcag aaggggccgc cgccgaggaa atccagcttg aacgacgact
     4201 ttgcctccag cggcgcgaag ccccgcgcct tcggcggacg atggatcggt tggggcgtca
     4261 cgggatcggg cggcggcgcg gcgctggcgc ccgccggccc gtacgccggc tttggcgaga
     4321 gccagcccgc cgattatggg cgcagcgccg aaaccggcgc gctcgccgtc tacaatctcg
     4381 gcaagagcta tagcggccgc gcggtcgtca aggacgtcag catctctgtg cggcgcggcg
     4441 aggcggtcgg cctgctcggg ccgaacggcg cgggcaagac cacggtcttc tacatgatca
     4501 ccggcctggt ccggccggat aagggcgtca tcgagcttga cggccatgac gtgaccgccc
     4561 tgccgatgta ccagcgcgcc cggctcggca tcggctatct gccgcaggaa gcctcgatct
     4621 tccgcggcct caatgtcgag gacaatatcc gcgcggtgct cgaagtgacc gagcccaacc
     4681 gcaagacgcg cgaagcgatg ctcgattcgc tgctcgaaga attcgacatc gcccggttgc
     4741 gaaaatcgcc gtccatcgcc ctgtcgggcg gcgaaaggcg gcgctgcgag atcgcccgcg
     4801 cccttgccgg caagccttcg ttcatgctgc tcgacgaacc cttcgccggc atcgacccga
     4861 tcgcggtcgg cgacatccag gaccttgtcc gccatctcaa gggccgcggc atcggcgtgc
     4921 tgatcaccga ccacaatgtc cgcgagacgc ttggcctgat cgaccgcgcc tatatcattc
     4981 atagcggcca tgtcctaaca gagggcggac cggacgagat catcgcgcat ccggatgtgc
     5041 gaaggttcta tctgggcgag gatttcagga tgtgaggagg ggggcgtacg tactccggac
     5101 gccttcggcc ctgcgccgtc tcgtcattgc gaggagcgaa gcgacggagc aatccatacg
     5161 tcgccgcgcc ctacgagcgg atcgtttcgc ttcgctcgca atcacagcag acgcgtattt
     5221 cccgggtcct gcgcagccgc caacgcgtga caacctcaga cttccccgga tagctctgcg
     5281 caaagcgggg agaagggatt tgcgccctat tcgtcctcgc cgaacctgct cgccaccagc
     5341 ttttcgatcg cgtcgagcgc cgcttcggcc tccgcgccgc tggccgagac ggtgatcgtc
     5401 gagcccatcg cggcgccgag ggtcagaatg cccatgatcg aatcgccgcc gaccgtctcg
     5461 ccgcagcgcg tcaccctgac ctcggcgtcg aagcggtcga cgcattggac gaatttggcg
     5521 gtcgcccgcg catgcaggcc cttgcggttg acgatgcgga tttcgcgcgc cagggcgccg
     5581 gcgaattcgg ggggcggcgg acaatcctct tcgcctgtcg cggcggcttc cgcttcgctc
     5641 atttgccggc caagaccttg ctcgcgacgg tgatgtattt gcgcccggcg tcgcgcgcct
     5701 cgccgaccgc atgttcaaga tcggcggtct ctctcaccga agcaagcttg atcagcatcg
     5761 gcaggttgat gccgcagatc acttcgacgc ggccgccatc catcaccgaa atggcgagat
     5821 tggagggggt gccgccgaac atgtcggtca gcaggacgac gccgccgcca tcctgaacgc
     5881 gcgagacggc gttcaaaatg tcgccgcggc gctgctccat atcgtcttcc gggccgatgg
     5941 cgatggtttc gaattgcgac tgcggcccga ccacgtgctc caaggcggct cgaaacccgc
     6001 tcgccaattg gccgtgggtc accaggacca ttccgatcat caacaacccg ctgatcttac
     6061 gccggcgctt aacgccgccc gtcgccgcgc ccggcgtgac aaagcgcggc atatttgtgc
     6121 actgcggcgt attattcaag caaaatgagc aatcctcgtc acaaagctgt catctcggcg
     6181 gcgagcgccg ccaaaaccgc gcgcgcccgc tcaaacgggc ggctcgactc gtcgaaagcg
     6241 agccgccgca ggcggatggg gccgaatgtc gcgacctcgt cggccgcctc gggcaggcgg
     6301 gcggcggcgt cgccgtcatc aagcaaatcg accgccagcc gcgccaccgc ggccggctcg
     6361 gccggcgtga tttccaggcc gacaccacgc cgctcgataa ggcccggtat ttgaggggcg
     6421 ccgcggagca acaaccggcc gttgcaggct tgaacaagaa tgcggtcgtc gccgatcaag
     6481 cggccgaaca agccccgctc gcccgcgagc tcgatcagcg ccagcgccag cgcgctcttg
//
LOCUS       DATGJW010000007         2717 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1349664, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000007 DATGJW010000000
VERSION     DATGJW010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2717)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2717)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2717
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1349664"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..720
                     /locus_tag="VKU03_00250"
     CDS             <1..720
                     /locus_tag="VKU03_00250"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ69716.1"
                     /translation="GEQFHFINDGYADFDAFLAALSSRKRKTIRRERAEALGDEVTVE
                     WITGPALGEKHWDAFFAFYMDTGSRKWGSPYLTRDFFSKVGAALADRILLILAKRKGR
                     YIAGALNFIGDDALYGRNWGAIEERPFLHFEICYYQAIDFALRRKLKRVEAGAQGEHK
                     LARGYRPVPTYSAHELADRRLAEAVADYLRRERQAVKETIAFYDEHAPFRRGDPLLPA
                     QRGEGGRAQHGRMGGSAEPAD"
     gene            complement(820..2508)
                     /locus_tag="VKU03_00255"
     CDS             complement(820..2508)
                     /locus_tag="VKU03_00255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HLJ69717.1"
                     /translation="MTKPHGLDGGLTNYGDRDFARFLRRSFARSMGVSRALLDKPVVG
                     IAMTPSGFNNCHRLMPDLVEAVSRGVLAAGALPRAFPTISLGEVFLNPTSMVYRNLMA
                     MDTEEMIIAQPMDAVVLIGGCDKTVPAQLMGAVSAGVPAIQLVTGPMSTGRHRGQRLG
                     ACTDCRAFWAKYRAGSVGAEEIAEVEGRLAVTAGTCAVMGTASTMACIAETLGMALPG
                     SAAIPAVHADRLVAAEETGRAAAALIANPIPPERIITEKSVENALRVLMALSGSTNAV
                     IHLAAIAGRLGLRLSLERLNQISDETPTLVDLKPVGQGYMEDFHAAGGLGAVLRELKP
                     LLHLETIDVTGRTLGARLAEPLNWIDRAVIRPFDDPIAKFGGLIALKGTLAPDGAILK
                     RAAATPALFEHEGRAVVFDGLEDLAKRIDDPRLDVTPDDVLVLKNAGPKAAGMPEAGY
                     LPIPKKLAKAGVKDMLRVSDARMSGTAFGTIVLHAAPEAAVGGPLAAARSGDRIRLSV
                     EKRRIDLLVADEEIRRRLAARQPPPTPARGYKALYAKSVTQAPDGCDFDFLMKR"
BASE COUNT          464 a          897 c          898 g          458 t
ORIGIN      
        1 ggcgagcagt ttcatttcat caacgacggc tatgccgatt tcgacgcgtt tctcgccgcg
       61 ctctcgtcgc gaaagcgcaa gacgatccgc cgcgagcgcg ccgaagcttt gggcgacgag
      121 gtgacggtcg aatggatcac cggcccggcg ctcggcgaaa agcattggga cgcgttcttc
      181 gccttctata tggataccgg ctcgcgcaag tggggctcgc cgtacctcac gcgcgatttt
      241 ttctccaagg tcggcgccgc cctcgccgac cgcatcctgc tcattctcgc caagcgaaag
      301 ggacggtaca tcgccggcgc gctgaatttc atcggcgacg acgcgcttta cggacgcaat
      361 tggggcgcga tcgaagagcg gccgttcctg catttcgaga tctgctacta ccaggcgatc
      421 gatttcgccc ttcggcgcaa gctgaagcgc gtcgaggccg gcgcgcaagg cgagcacaag
      481 ctggcgcgcg gttatcggcc cgtgccgacc tactcggcgc atgagctcgc cgaccgccgt
      541 ctcgccgagg ccgtggcgga ctatctgcgt cgcgagcgac aagcggtgaa agagacgatc
      601 gccttctacg acgagcatgc gccgttccgg aggggcgatc cccttctccc agcgcagcgg
      661 ggagaaggcg gccgtgcgca gcacggccgg atggggggca gcgctgaacc tgccgattag
      721 actagccctt cgaaaggggc gcgtactttc tgagtcctgc gccgcccctc atgccgccgc
      781 ttcgcggcca ccttctccac gtggagccgg tagaagtgtt caccgcttca tcaggaaatc
      841 gaagtcgcat ccatccggcg cttgcgtgac gcttttcgca tagagcgctt tgtaccctcg
      901 tgcgggtgtg ggcggcggct ggcgtgcggc gagccgccgg cggatttcct catccgccac
      961 caggagatcg atgcgcctct tctcaactga taagcggatg cggtcgccgc tccgcgccgc
     1021 cgcgagcgga ccgcccaccg ccgcctcggg cgcggcgtga agcacgatcg tcccgaacgc
     1081 cgtgcccgac atgcgcgcat cggagacgcg cagcatgtcc ttgacgccgg ccttggcgag
     1141 ctttttcggg atcggcaaat agccggcctc cggcatgccg gccgctttcg ggccggcgtt
     1201 cttgagcacc agcacgtcgt ccggcgtcac gtcgaggcgg ggatcgtcga ttctcttggc
     1261 caaatcttcg agcccgtcga agacgaccgc cctgccctca tgctcgaaca atgccggcgt
     1321 cgccgccgcg cgcttcagaa tagcgccgtc cggcgccagc gtccctttca gcgcaatcag
     1381 tcccccgaat ttggcgatcg gatcatcgaa gggccggatg acggcgcgat cgatccagtt
     1441 cagcggctcg gcgagcctcg cgccaagcgt gcgtcccgtc acgtcaatcg tctcgagatg
     1501 cagcaagggc ttgagctcgc gcaagacggc gcctaggccg ccggcggcgt gaaaatcctc
     1561 catatagcct tggccgaccg gcttgagatc gacaagggtc ggcgtctcgt ccgagatttg
     1621 attgagccgt tcgagcgata ggcgaaggcc gagccttccg gcaatggctg cgagatgaat
     1681 gaccgcattg gtcgagccgc tcagcgccat cagcacgcgc aacgcgttct cgaccgattt
     1741 ctcggtgatg atccgctccg gcgggatcgg attggcgatg agcgcggccg cggcgcggcc
     1801 ggtctcctcg gcggcgacca gccgatcggc atggacggcg gggatcgcgg cgcttcccgg
     1861 cagagccatg ccgagcgttt cggcgatgca ggccatcgtg ctcgccgtac ccatcaccgc
     1921 gcaggttccc gcggtcacgg cgagacggcc ctcgacttcg gcgatctcct cggcgccgac
     1981 gctcccggcc cgatatttcg cccagaaggc gcggcaatcg gtgcaggcgc cgaggcgttg
     2041 gccgcgatgg cggccggtcg acatgggccc ggtgacgagc tggatcgccg ggacgccggc
     2101 cgagaccgcg cccatcaatt gcgccggcac ggtcttgtcg caaccgccga tcagcacgac
     2161 cgcgtccatc ggctgggcga tgatcatctc ctccgtgtcc atcgccatca ggttgcgata
     2221 gaccatgctg gtcgggttca ggaacacttc gccgagcgag atggtcggaa aggcgcgcgg
     2281 caaggcgccc gcagcgagaa cgccgcgcga cacggcctcg accagatcgg gcattaggcg
     2341 gtggcaattg ttgaaaccgg acggcgtcat cgcaatgccg accaccggct tgtcgagaag
     2401 cgcgcgcgag acgcccatcg agcgcgcgaa ggagcggcgc aggaaacggg cgaaatcgcg
     2461 gtcgccgtaa ttggtgaggc ctccgtcgag gccgtgcggc ttggtcattg cggattcccg
     2521 gattttgaac ggcccagtgt cgcgcgccgg cggaaaagcg gcaatgcggg gcggctcgcg
     2581 ccgcgcaaaa gccattttca cccggttcag atttagaaat agtctaaaca gaggcgcgat
     2641 tctcaacgcg agctggcgcc gcgccatcaa aggccctgcg aggagcgatg acggaagaac
     2701 gaccgatccg ttacgat
//
LOCUS       DATGJW010000008         9586 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1453456, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000008 DATGJW010000000
VERSION     DATGJW010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9586)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9586)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9586
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1453456"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..386)
                     /locus_tag="VKU03_00260"
                     /pseudo
     CDS             complement(6..386)
                     /locus_tag="VKU03_00260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974950.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase N-terminal
                     domain-containing protein"
     gene            530..1069
                     /locus_tag="VKU03_00265"
     CDS             530..1069
                     /locus_tag="VKU03_00265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HLJ69718.1"
                     /translation="MVKSAASPHASRGIGPIVSSAHLAEGASPALSEFEFGLILASHA
                     FLRWIVRGMNAAGYPDLSTIDVMVLHSVAHRERRKRQADICLVLNIEDTHLVAYAIKK
                     LERLGLVSSEIVAKEKLVSVSERGEAACRAYREIRERLLVEPVKAMGFEESRLSKLAA
                     RLRALSGHYDQAARAAASL"
     gene            1184..1312
                     /locus_tag="VKU03_00270"
     CDS             1184..1312
                     /locus_tag="VKU03_00270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69719.1"
                     /translation="MSAHLVSKSGAGAAVNLDGLAAAATPSSSRERGNKDGERKTP"
     gene            complement(1330..2058)
                     /locus_tag="VKU03_00275"
     CDS             complement(1330..2058)
                     /locus_tag="VKU03_00275"
                     /inference="COORDINATES: protein motif:HMM:NF018928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SapC family protein"
                     /protein_id="HLJ69720.1"
                     /translation="MADAPLFYQNIVPLDRERHRKLRLVQNQRPFAFAAKSHMIPAVV
                     DEFVMASRFLPIVFLPSSPHPSPVFLVGARTGQNLYVDKDGKWAEGYVPAFVRRYPFM
                     LGTPENGAPIICVDEKHEGLRQNGAGDRLFSEDGAETPVLQEFAKLTESYFAAAKRTE
                     TFLETISALQLLRGVTIEMRPEPGSSNALHGFMTIDEARLNALPDQDFLRLRAEGWLA
                     HIYAHLFSLGVIDRLRRLSAPANA"
     gene            2360..3070
                     /locus_tag="VKU03_00280"
     CDS             2360..3070
                     /locus_tag="VKU03_00280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69721.1"
                     /translation="MRRPALRLFAAAGLAVWTGAFTGGALAETPAPNAGVAAPQPATG
                     STPIAEPSESRKLELTGFRSALFGMSEAEVRGAILKDFGVATASAVKASQNAAERTQV
                     LTIKAPDVLSDGGIAEVSYVFGYKTKKLIQIGVLWSKATDEKMTAERLVSDADALRAY
                     FLTAGYEPQTIAQNLAVPNGVLMFRGSDAAGHTTLLLLHGVTHEKPSHEFSPTALSLF
                     YVADVKNPDVFKVQPGKF"
     gene            3131..4759
                     /locus_tag="VKU03_00285"
     CDS             3131..4759
                     /locus_tag="VKU03_00285"
                     /inference="COORDINATES: protein motif:HMM:NF015803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="POTRA domain-containing protein"
                     /protein_id="HLJ69722.1"
                     /translation="MQKLIGAAAFAGAAMAPLSAAAQTPPPPNPGLISNQNQQNREQL
                     EQRNALPQGPAVLAPPPYQSAVGPAGGPTFVLRGVKVEASHFLSKREIDAITGQYIGK
                     RVDISAVQRMVKEINDLYAARGIVTAAAYLPPQKLKDGIVTIKIVEGRLGRLKVTGAN
                     QLSPDFVLSHVDQKVGEVVDVPRMSRELAFFNKTGVARIQALMQPGAQFGLTDVELSV
                     LEPPKNLIQIFGDNMGVSSVGSLEAGTLLQHYGILGLDDKLTLYAVKSEGNFNGNVSY
                     NLAVDPWGGRLGFSLTRGAIRVISGPYKSLDVDGTAETASINASQPVLGNAEWMLLLN
                     GSLSRVVSTSDQTHVPVTGDYTSLQTGGVTLAYTGANVSASVSPTLSAAQSYSDVTGV
                     KENFFLQSGTFSSLAQLPYNFSATLAGAWQTSSKELLPGDQLFQLGGPTTVRGYPTDA
                     VAGPDGYYANLELHHPIPGPMSTALDAYVFYDRGSVFNHFPAVQTLNAIGAGVSWTVG
                     KYAVAEVSAGFPLNTVVEPQAPYEIYFRLTAKID"
     gene            4976..6052
                     /locus_tag="VKU03_00290"
     CDS             4976..6052
                     /locus_tag="VKU03_00290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003591812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLJ69723.1"
                     /translation="MAQKSFRAPARQAAKRGGKQLAGALTMQKIAELAGVSAMTVSRA
                     LKSDASILPETRNRVLDIVSRIGYVPDATARIFASRRSGFIAALVPSLNNSNFAETVR
                     GMEETFAPVGLQMLLGDTEYSLAREEDLIGAFLQRRPEAMVLTGGVHTARARQMLAGA
                     GIPIVETWDLPKAPLDHVVGFSNEAAGAAMVRYLHGRGRRRIGFIGGATTLDTRGSER
                     RAGYARAIRELGLPRGRIVTFGKPPVSMSQGAEALDLMLKQWPDVDAILCVSDLSAFG
                     VLMECQRRGLDVPGQLAIAGFGDFEVARCCHPRLTTIAVDCQGIGRRAAEIALNALQV
                     KKQGEALPPETVVMEFRVVARETA"
     gene            complement(6161..7192)
                     /locus_tag="VKU03_00295"
     CDS             complement(6161..7192)
                     /locus_tag="VKU03_00295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008133307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-idonate 5-dehydrogenase"
                     /protein_id="HLJ69724.1"
                     /translation="MRAVVAHAAKDLRIEELPDQQIGEKDVRVRIAFGGVCGSDLHYF
                     NHGGFGQVRLREPMVLGHEVAGIVEEIGREVRRIKVGDRIAVSPSQPCNQCRYCLEGQ
                     QNQCLDMRFYGSAMRFPHIQGAFRETLVCRQEQAHVVAPGVSLGEAAMAEPLAVCLHA
                     ARRAGPLLGKRVLVIGAGPIGALCVAAARRAGAAEIVITDIADGVLDFAARVRPDRIV
                     NTAKEPDALSAYAHDKGYFDTLFEASGAAVALRGAIDVVRPGGALVQVGLGGDMTLPI
                     NVIVAKELQLRGTFRFHEEFATAVEFIGKGLVDVKPLISATLPFVEARQAFELAGDRS
                     RSMKVQLQF"
     gene            7281..8057
                     /locus_tag="VKU03_00300"
     CDS             7281..8057
                     /locus_tag="VKU03_00300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ69725.1"
                     /translation="MGQLIERLFGLSGRIALITGSSGGIGEALARGLAEAGAQVVVNG
                     RTAAKVDGVVASIAAAGGSAAGAVFDVTDPDAVHGAIARIERDIGPIDILVNNAGIQR
                     RKPLEDFPVETWRELMKANLDSVFFVGQAAARFMIPRKRGRIINICSVQSELARSSIA
                     PYTASKGAVKMLTKGMATDWGKYGICVNAIGPGYFRTELNKALVVDDKFSAWLAGRTP
                     LGRWGEVDELVGAAIFLASDAASFVNGHVLYVDGGITSTL"
     gene            8192..>9586
                     /locus_tag="VKU03_00305"
     CDS             8192..>9586
                     /locus_tag="VKU03_00305"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ69726.1"
                     /translation="MSAAPTAKPETLIGFRDIAKTFGGVVALRGVSLDLQAGEILALL
                     GENGAGKSTLIKILAGIFEPSRGEIVYRGEPYRHRPPKFGERQPVAFIHQDLGLIEWM
                     TVAENIGLATGFPRKYGVVDWRAVGVATERALAQVDCRLDPTTRIRDLSRTEKSLVAI
                     ARALSVEADVLVLDEPSASLPADEVHRLFDALRSLKARGVGMIYVSHRLDEVFEIADR
                     VAVLRDGALVGERRVGETDPEELVSLIVGKALERYEGARLARRGAARVEAREFRVDGA
                     GPVSFEVAQGEILGLVGLRGAGQERIGRALFGTLPHSGTLLIDGAPAAFRSPAEAMTA
                     GIGLIARDRVEESTANGLTIRENTFLNPAALGRGVVSFLPPRREAERARDIGFRVGLR
                     PNLPDLPIEALSGGNQQKVIVGRWLEIAKRLLIAEDPTAGVDIGAKAEIYRLLDKALS
                     GGLGIVVVSTDFEEV"
BASE COUNT         1702 a         3055 c         3104 g         1725 t
ORIGIN      
        1 atctagattt tcggccgcgc ctgatagccg atttctagcg cgtcgcgaaa gccgcgcgtc
       61 gtggcgagca aggtccgcac gcccttgcgc tcgagcaaag cgttggtcgc gaccgtggtc
      121 cccatcttca ccgcgccgat cgtcccggtc ccgatcggcg cgcccggttg gagcccaaga
      181 agatcgcgga tcgcctgcac ggcggcgtcg ctataggccg gattctcgga cagaagcttg
      241 cggacgtgga ggcgcccttg tgggtcgcgg ccgatcacat cggtgaacgt accgccgcga
      301 tcgacccaga agtcccacgc gcctgtgacg cttgtgtgat ggccaacgcg cttcgccacg
      361 aacgccccct gcgccaactc cgtcatcgca agcgaagcga agcaatccat tcgtcgggca
      421 gaaccttacg tgcggattgc ttcgtcgctc cgctcctcgc aatgaggatg tcgattgaca
      481 atatagataa tttatcgata aattatctat aacgcaaggc tacgaaagca tggtcaaatc
      541 ggcggcatcg cctcatgcgt cgcggggaat cggcccgatc gtctcttcgg cgcatctcgc
      601 cgaaggggcc agcccggctt tgtcggaatt cgaattcggc ctgattctgg cgagccacgc
      661 cttcttgcgc tggatcgtcc gcggcatgaa tgcggccggc tatcccgatc tgtcgaccat
      721 cgatgtgatg gtcttgcatt cagtcgccca tcgcgagcgg cgcaagcgcc aggctgatat
      781 ctgcctggtg ctcaatatcg aagacaccca tctcgtcgct tatgcgatca agaaattgga
      841 gcgccttggc ctcgtgagca gcgagatcgt cgccaaggaa aagctcgtct cggtcagcga
      901 gcgcggcgaa gcggcctgcc gcgcctatcg ggaaatccgc gagcgcctgc tggtcgagcc
      961 ggtcaaggcg atgggtttcg aagaaagccg gctgtcgaag ctcgcggcgc gcctgcgcgc
     1021 gctgtccggc cattacgatc aagcggcgcg cgccgcggcg agcttgtgac gggtcatcgc
     1081 gcagccgggg ttttgtagcg cggccgttgg ccgcgtcagc cgcgctcgtg gagcgcggct
     1141 acatcccgcc cacgaggcgc gccggctctg gtggtgcgcc gcgatgagcg cgcatctggt
     1201 gagcaagagc ggcgctggcg cggcggtcaa tctcgacggc ctcgccgcag cggctacacc
     1261 gtcgtcttca cgcgaacgcg gcaataagga cggcgaacgg aaaacgccgt gacccggctc
     1321 ggcccgcggt caggcgttgg ccggcgccga gaggcggcgc aagcgatcga tgacgccgag
     1381 gctgaaaagg tgagcataga tatgcgccag ccagccttcg gcgcgcagcc ggaggaaatc
     1441 ctggtcgggc agagcgttga gccgcgcctc gtcgatcgtc atgaagccgt gaagcgcgtt
     1501 gctcgatcca ggctccggcc gcatctcgat tgtcacgccg cgcaggagct gcagcgcgct
     1561 gatcgtttcg aggaacgtct cggttctctt cgccgctgcg aaatagcttt cggtgagctt
     1621 ggcgaattcc tgcaggaccg gcgtctcggc gccgtcttca gagaacagcc ggtcgccagc
     1681 gccgttttgc cgcaggccct catgtttttc gtcgacgcag atgatcggcg cgccgttttc
     1741 cggcgtgccc agcatgaacg ggtagcggcg gacgaaggcg ggaacgtagc cttcggccca
     1801 tttgccgtcc ttgtcgacat agaggttctg tccggttcgc gcgccgacca ggaagactgg
     1861 cgagggatgc ggcgaagagg gaagaaagac gatcggcaga aaccgggacg ccatgacgaa
     1921 ttcgtcgacc accgccggga tcatgtgaga cttcgctgcg aacgcgaacg gcctttggtt
     1981 ttgaaccagg cgaagcttgc ggtgacgctc tcgatcgagg ggaacgatgt tttgatagaa
     2041 aagaggagcg tcagccatca gctttattcg ctttctaaag acaagaccgc ggacgataac
     2101 gcgccgctat tcgcgcgatc aataggagcg gttgagaata attaatgatc ctagcggcga
     2161 tccctctttt ttcttgtcgc aaacgcggcg atctgctttt gatgagtcgg gaggcctctt
     2221 cgcgccttgc gcaatcggcc ctcttaaaac aatgagcccc agcgggcgcc tccgcggctg
     2281 agaaagcaag aggggtttcg ccagcagccg tagcgggcgc cgtcagtggg gtcggtgggt
     2341 catgagcgtg agaatgatca tgaggaggcc cgcgctgcgg ctgttcgcgg ctgcagggct
     2401 tgcggtctgg acgggcgcct ttacaggcgg cgctttggcc gagacgccgg cgccgaatgc
     2461 cggcgtcgct gcgcctcagc cggcgacggg atcgacgccg atcgccgagc caagcgagtc
     2521 gcgcaagctt gaattgaccg gctttcgctc cgcgcttttc ggcatgtccg aggcggaagt
     2581 ccgcggcgcc atcctgaagg atttcggcgt cgcgacggcg agcgccgtca aggcctcgca
     2641 aaacgccgcc gagcgcacgc aagtcttaac gatcaaggcg cccgacgttt tgtcggacgg
     2701 cggaatcgcc gaagtctcct atgtcttcgg ctacaagaca aagaagctca tccagatcgg
     2761 cgtgctgtgg tccaaagcga ccgacgagaa gatgacggcg gagcgcctgg tttccgacgc
     2821 cgatgcgctg cgcgcctatt tcctcaccgc cggctacgag ccgcagacga tcgctcaaaa
     2881 tcttgccgtg cccaatggcg tgttgatgtt ccgcggcagc gacgccgccg gccacacgac
     2941 gctcttgctc ctgcatgggg tgacgcatga aaagccgtcg catgaatttt ctccgacggc
     3001 gttgagcttg ttctatgtgg cggatgtgaa gaatcccgac gttttcaagg tgcagccggg
     3061 caagttctga tcgaaacgcc gatcgggcgc aaaagcgggg agactttcct tgggcggggc
     3121 tggggggcgc ttgcaaaagc tgattggcgc ggccgctttt gccggcgcgg cgatggcgcc
     3181 gctttcggcc gccgcgcaaa cgccgccgcc gcccaatccc ggcctgatct ccaatcagaa
     3241 ccagcagaac cgcgagcaat tggagcagcg gaacgcgctg ccgcaggggc ctgcggtgct
     3301 ggcgccgccg ccctatcaaa gcgcggtggg cccggcgggc gggccgacat tcgtgctgcg
     3361 cggcgtcaaa gtcgaagcgt cgcatttcct gtcgaagcgc gagatcgacg cgatcaccgg
     3421 ccaatatatc ggcaagcgcg tcgacatatc cgccgttcag aggatggtga aggagatcaa
     3481 cgatctctac gccgcgcggg gcatcgttac ggccgccgcc tatctgccgc cgcagaagct
     3541 gaaagacgga atcgtcacga tcaagatcgt cgaagggcgg cttgggcggc tgaaagtgac
     3601 gggcgcgaac cagctctcgc ccgatttcgt cctgtctcat gtcgatcaga aagtcggcga
     3661 agtggtcgat gtgccgcgca tgtcgcgcga gctcgctttc ttcaacaaga ccggcgtcgc
     3721 ccgcatccag gccttgatgc agcccggcgc gcaattcggc ctgaccgatg tcgagctctc
     3781 cgtcctcgag ccgccgaaaa atctcatcca gatattcggc gacaatatgg gcgtctcgtc
     3841 agtcgggagc ctcgaagccg gcacgttgct tcagcattat ggaatcctcg gccttgacga
     3901 caaattgacg ctctacgccg tcaagtccga gggcaatttc aatggcaacg tctcctacaa
     3961 tctggcggtc gatccttggg gcgggcgtct tggattcagc ctcacccgcg gggccatccg
     4021 cgtcatctcc ggcccgtaca aatccctcga tgtcgacggt acggcggaaa ccgcttcgat
     4081 caacgccagc cagcccgtcc tcggcaatgc cgaatggatg cttctcctca acggttcgct
     4141 aagccgcgtc gtttcaacgt ccgaccagac ccatgttccg gtcacgggcg actatacgag
     4201 cttgcagacg ggcggcgtca ccttggccta tacgggcgcg aacgtctccg cgagcgtctc
     4261 gccgaccctc tccgccgctc agtcctatag cgacgtgacg ggcgtaaagg agaatttctt
     4321 cctgcagagc gggacgttca gctcgctggc ccagcttccc tataatttca gcgcgacgct
     4381 ggccggcgct tggcaaacct cgtccaagga gcttttgccc ggcgatcagt tgttccagct
     4441 cggcggcccg acgacggtgc gcggctatcc gaccgacgcg gtcgcgggtc cggacggcta
     4501 ttacgccaat ctcgaattgc atcatccgat ccctggtccg atgagcaccg ccttggacgc
     4561 ttatgtcttt tacgatcgcg gctcggtctt caatcatttc ccggccgtgc agaccctgaa
     4621 cgcgatcggc gccggcgtct cctggactgt cggcaaatac gccgtcgcgg aagtcagcgc
     4681 cggcttcccg ctcaacactg tcgtcgagcc gcaagcgcct tatgaaatct atttccggct
     4741 gaccgcgaag atcgattgaa cgcggccgcg cttgcggcga agcattgaat ttcaaacgat
     4801 cgttcggcct gcgttgtttt ctcgcaacgc attcgcccgt tcgacctgct cagtctgtcg
     4861 gcctgcgcgc tcccatgcgc tcgacaaccg ctcgtgccga tgttatcgat accatgcgcg
     4921 cggatcggcg atcgcctgcg ttcgattgcg cgtcgagagc agagaagggt cgagcatggc
     4981 tcaaaagagc ttccgtgcgc cggcgcggca ggccgcaaag cgcggcggaa agcagttggc
     5041 cggcgcgctg accatgcaaa agatcgccga gctcgccggc gtttcggcca tgaccgtctc
     5101 gcgcgcgctg aagagcgacg cgtcgatcct gccggaaacg cgcaaccgcg tcctcgatat
     5161 cgtcagccgc atcggctatg tgcccgacgc caccgcgcgc atcttcgcca gccggcgctc
     5221 cggctttatc gctgcgctgg tgccgtcgct caacaactcc aatttcgccg agacagtgcg
     5281 cggcatggag gagacgttcg ccccggtcgg cttgcagatg ctgctcggcg ataccgaata
     5341 ttccctcgcc cgcgaggagg atctgatcgg cgcgttcttg cagcggcggc cggaggcgat
     5401 ggttctgacc ggcggcgtgc atacggcccg cgcccgccag atgctagccg gagccggcat
     5461 tccaatcgtc gagacatggg acttgccgaa agccccgctc gatcacgtcg tcggcttctc
     5521 caacgaagcg gccggcgcgg cgatggtgcg ctatctgcac gggcgcggcc gacgcaggat
     5581 cggcttcatc ggcggcgcga ccactctcga cacgcgcggg tcggagcgcc gcgccggcta
     5641 tgcgcgggcg atccgcgagc tcggcctgcc gcgcggccgc atcgtcacgt tcggcaagcc
     5701 gccggtctcg atgagccaag gcgccgaagc gctggacttg atgctgaagc aatggccgga
     5761 tgtcgacgcc attctttgcg tctccgatct gtcggctttc ggcgtgctga tggagtgtca
     5821 gaggcgcggc ctcgacgtgc cggggcagtt ggcgattgcc ggctttggcg atttcgaagt
     5881 ggcgcgctgc tgccatcctc gcttgaccac gatcgccgtc gattgccaag gcatcggccg
     5941 acgcgccgcc gaaatcgccc tcaacgcgct ccaagtgaag aagcaaggcg aggcgttgcc
     6001 gcccgaaacc gtggtgatgg agttccgggt tgtcgcgcgg gagacggcgt aggacgcccc
     6061 gtgtcgctcc cgcgcaagcg gaaatccagt acgatctatc ccgcaacgct gagactcctg
     6121 gattcgcgct tgcgcgggac taaaccaagg ttcagtgccg ctagaactgg agctgtacct
     6181 tcatcgagcg cgagcgatcg cccgccaatt cgaaggcttg gcgcgcctcc acgaagggca
     6241 gggtcgcgga aatcagcggc ttcacgtcga cgaggccctt gccgatgaat tccacggcgg
     6301 ttgcgaactc ctcgtggaag cggaacgtgc cgcgcaattg cagctccttg gcgacgatga
     6361 cgttgatcgg cagcgtcatg tcgccgccaa gtccgacttg gacaagcgcg ccgccgggcc
     6421 gcacgacgtc gatcgcgccg cgcagcgcca cggccgcccc cgacgcctcg aacaaggtat
     6481 cgaagtagcc tttgtcgtgc gcgtaagccg aaagcgcgtc gggctctttc gcggtgttaa
     6541 cgatccggtc gggcctgacg cgggcggcga aatcgagcac gccgtcggca atatcggtga
     6601 tcacgatttc agccgcgccg gcgcgccgcg ccgccgcgac gcagagcgcg ccgatggggc
     6661 cggcgccgat gacgagcacg cgcttgccga gcaaggggcc ggcgcgccgc gcagcgtgca
     6721 ggcacaccgc cagcggctca gccatggccg cctcgcccag cgagacgccc ggcgcgacga
     6781 catgggcctg ctcttggcgg cagacgaggg tctcgcggaa tgcgccctgg atgtgaggga
     6841 agcgcatcgc gctgccatag aagcgcatat ccaaacattg gttctgctgg ccttcgaggc
     6901 agtagcggca ctgattgcac ggttggctcg gactgaccgc gatacggtcc ccgaccttga
     6961 tgcgccgaac ctcccgcccg atctcctcga caatgccggc gacctcatgg ccgagcacca
     7021 tcggctcgcg caggcgcact tggccaaagc cgccatggtt gaaatagtgc agatcggagc
     7081 cgcagacgcc gccaaaggcg atgcgcacac gaacatcctt ttcgccgatc tgttggtcgg
     7141 gcagctcctc gatccggaga tctttcgccg catgggcgac cacagcgcgc ataggaactc
     7201 ccttgactcg agtcgtgtta tcgataacat aaaccgagcg aaagcgcaaa gtctcgaccg
     7261 gagacaagga ggaagcccga atgggccagc tcatcgagcg gttgttcgga cttagcggcc
     7321 gcatcgctct aatcaccgga tcatccggcg ggatcggcga ggccctggcg cgcggcttgg
     7381 ccgaagcagg cgcgcaggtc gtcgtcaatg gacgcacggc ggcgaaggtc gacggcgtcg
     7441 tcgcttcaat cgccgccgcc ggcggaagcg ccgccggcgc ggttttcgac gtcaccgatc
     7501 ctgacgccgt ccacggcgcg atcgcgcgta tcgagcgcga catcggccct atcgacatcc
     7561 tcgtgaacaa tgccggcatc cagaggcgta agccgctcga agattttccg gtcgaaacct
     7621 ggcgcgaatt gatgaaagcc aatctcgaca gcgtcttttt cgtcggccag gcggcggcgc
     7681 gctttatgat ccctcgcaag cgcggccgca tcatcaatat ctgctcggtg caaagcgaac
     7741 tcgctcggtc gagcatcgcg ccctacaccg cctcgaaagg cgccgtgaaa atgctgacca
     7801 aaggcatggc gaccgattgg ggaaagtacg ggatttgcgt aaacgcaatc ggcccaggct
     7861 actttcgcac cgagctcaac aaggcgctgg tcgtcgacga taaattctcg gcctggcttg
     7921 ccgggcggac gccgctcggc cgctggggcg aggtcgacga actcgtcggc gcggcgatct
     7981 ttctcgcctc cgacgccgcg agcttcgtga atggccatgt cctctatgtc gatggcggca
     8041 taacgtcgac gctctgacag cgcgcgagca gcggcgcgtg gcggcgccgc gccgctttca
     8101 aatcacgtca aaagaaatcg cttgccagcc cttcttgtta ccgataacat aaagaaccta
     8161 gaaatcgccg cggagcgaat aaggggagga tatgtccgcc gcgccgaccg ccaagccgga
     8221 gaccttgatc ggctttcgcg acatcgccaa gacgttcggc ggcgtcgtag cgctgcgcgg
     8281 cgtgagcctg gatttgcaag ctggcgaaat cctcgctttg ctcggcgaga acggcgccgg
     8341 caaatcgacg ctcatcaaaa tcctcgccgg catattcgag ccgagccgcg gcgagatcgt
     8401 ctatcgcggc gagccgtatc gtcatcggcc gccgaaattc ggcgagcgcc agccggtcgc
     8461 cttcattcac caggatctcg gcctgatcga atggatgacc gtggcggaga atatcggtct
     8521 tgcgaccggc ttcccgcgaa aatacggcgt cgtcgattgg cgcgcggtgg gcgtggcgac
     8581 tgagcgcgcc ttggcgcaag ttgattgtcg cctcgatccg acgacccgca ttcgcgacct
     8641 ctcacgcacc gaaaaatctc tcgtcgcgat tgctcgcgcg ctcagcgtcg aagctgacgt
     8701 gctggtgctg gacgagccgt ccgcgtcgct gccggcggac gaagtgcatc ggctcttcga
     8761 cgcattgcgg tcgctgaaag cgcgcggcgt cgggatgatc tacgtctccc atcggctcga
     8821 cgaagtgttc gagatcgctg accgggtcgc cgtgttgcgc gacggggcgc tggtcggtga
     8881 gcgccgggtc ggcgaaaccg atccggagga gcttgtttcg ctgatcgtcg gcaaggcgct
     8941 tgagcggtac gaaggggcgc gcctcgccag gcggggcgct gcgcgcgtcg aagcgcgcga
     9001 atttcgcgtc gacggcgctg ggccggtgag cttcgaggtc gcgcaaggcg agatcttggg
     9061 cctggtcggc ttgcgcggcg cgggccaaga gcggatcggg cgcgccttgt tcgggacgct
     9121 gccgcattcg ggaacgctgc tcatcgacgg cgcgccggcc gcgttccgct ctccggctga
     9181 ggcgatgacc gccggcatag gcctgatcgc ccgcgaccgg gtcgaggaat cgacggctaa
     9241 cgggctcacc atccgagaaa atacttttct caatcccgcc gcgcttggcc gcggcgtcgt
     9301 ctcgttcttg ccgccgcgcc gcgaggccga acgcgcgcgc gacatcggct tccgcgtcgg
     9361 cttgcggccg aacctgccgg atttgccgat cgaagcgctg tccggcggca atcagcaaaa
     9421 ggtcatcgtc gggcgctggc tggaaatcgc caagcgattg ctgatcgccg aggacccgac
     9481 ggccggcgtc gatatcggcg ccaaggctga gatttatcgg ctgctcgata aggcgctttc
     9541 cggcggactc ggcatcgtcg tcgtctcgac cgatttcgag gaggtc
//
LOCUS       DATGJW010000009         4476 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_934776, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000009 DATGJW010000000
VERSION     DATGJW010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4476)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4476)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4476
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_934776"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..757)
                     /locus_tag="VKU03_00310"
     CDS             complement(<1..757)
                     /locus_tag="VKU03_00310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF72 domain-containing protein"
                     /protein_id="HLJ69727.1"
                     /translation="MSKGAKPRIHIGIGGWTYEPWRGTFYPDGLAQKRELEYASRKLS
                     SIEINGTYYGLQKPASFIKWRDETPDGFVFALKGTRFITNRRALAEAGDSLKAFFDSG
                     VAELGAKLGPINWQFPPFKKFDAEDFEAFLKLLPKTAGGVELRHAVEVRHESFRSPAF
                     IALARKYGVAVIIAADSDYPQIADVTAPFVYIRIMGTTKEDKLGYSDKALDLWAKRAR
                     EWAAGGAPDGLKTVAEHQPEKAGRDVYLYVISGF"
     gene            complement(783..1508)
                     /gene="pgl"
                     /locus_tag="VKU03_00315"
     CDS             complement(783..1508)
                     /gene="pgl"
                     /locus_tag="VKU03_00315"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017057 - 6-phosphogluconolactonase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HLJ69728.1"
                     /translation="MTALAADLETAPIVRRDFPSSESLAEAFAEWSASLLQEAVAAHG
                     EATFIVSGGKTPELYFERLSHQDIDWSRVNVTLADERRVSENSPRSNAKLVREHLLQN
                     RAAEARFAPLADNRLSPEQELAAAQARIASLPLPADLVVLGMGDNGHTASWFPGGDNL
                     EAAINPGERALVLPMNAPDAAEPRLTLTARVILRAQAIALHIAGEEKRAVLKKALGEG
                     PAEELPIRAVLRGAPEKLTIFET"
     gene            complement(1505..2980)
                     /gene="zwf"
                     /locus_tag="VKU03_00320"
     CDS             complement(1505..2980)
                     /gene="zwf"
                     /locus_tag="VKU03_00320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLJ69729.1"
                     /translation="MTSRIIKVDPFDLVVFGGTGDLAYRKLFPALFHRDADGQFTDPT
                     RIVGVSRRPLDNEEFRASVKDALWKFAGVKDGAEPIVERFLRRIDYVPVDATGDAGWD
                     RLKALLDGENKVRAFYLATGPDLFGPIAKKLAANGMATPQARIIVEKPIGKDGASAAA
                     INDELGAVFAENRIFRIDHYLGKETVQNLMALRFANALFEPLWNAAHIDHVQITVAET
                     LGVEGRGAYYDGAGALRDMTQNHMLQLLCLVAMEPPRSLDADALRDEKLKVLKSLEPI
                     NAANAAAVTVRGQYRAGIADGKPVPSYLDDIGKESSDTETFVALKIGIANWRWAGVPF
                     FLRTGKRLPQRFSEIVIGFRRVPHAIFESNSDGIPANRLVIRVQPDEGIKLWLMIKEP
                     GAGGMRLQHVPLDMSFAKAFAGLSIDAYERLLMDVVRGNATLFMRRDEVEAAWRFIDP
                     IREAWAQLRESPRPYAAGSWGPAASIALIERDGRTWNEEFE"
     gene            complement(2992..4248)
                     /locus_tag="VKU03_00325"
     CDS             complement(2992..4248)
                     /locus_tag="VKU03_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018898259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HLJ69730.1"
                     /translation="MALGETEIVDTRLPAPVGADQTGVRLYNERLILSLMRRRRHLSK
                     IEVARQTGLSVQTASAIMNQLHADGLLKRETPQRGKVGQPTVPFSLDPEGAFSLGLKI
                     GRRSCDLVLIDFLGAIRERAHETFAYPQPGSTLGFVARALPGLTQGLTSRQRARLTGL
                     GVATPFYLWNWGAEVGAPPGAMDVWRNVDIKSEIASLCPVPVILCNDASSACAAEFFF
                     GEGWRHHDFLYFFVGSFIGGGVVIDGALFTGRTGTAGALGPIPIMRIENGRLVARQLI
                     HCASIYRLEERLRAAGVDPSSIWRTPNAWDDFSIHLDGWIEETAEALAYACLAAISVV
                     DFEAVIVDGAMPIAVRARLVTRIAEIYESLDRRGLVDPTIKAGSIGADARALGGAALP
                     LIANFARDREVLFKEPPTGSLSPNLP"
BASE COUNT          797 a         1446 c         1465 g          768 t
ORIGIN      
        1 tgaagccgct gatcacataa aggtacacgt cgcggcccgc tttttccggc tgatgctcgg
       61 cgacggtctt caggccgtcc ggcgcgccgc ccgccgccca ttcgcgcgcc cgcttcgccc
      121 agaggtcgag cgccttgtcg gaatagccga gcttgtcttc cttggtcgtc cccatgatgc
      181 ggatatagac gaacggcgcc gtcacgtcgg cgatctgcgg atagtcgctg tcggcggcga
      241 tgatgacggc gacgccgtat ttgcgcgcca gagcgatgaa agccggcgag cggaagcttt
      301 catgccggac ttcgacagcg tgacgaagtt cgacgccgcc ggcggtcttg ggcagcagct
      361 tcaggaaagc ctcgaaatcc tcggcgtcga atttcttgaa cggcgggaac tgccagttga
      421 tcggcccgag cttggcgccg agttcggcga cgccgctgtc gaagaaggcc ttgagcgaat
      481 cgccggcctc ggccagcgcg cgccgattgg tgatgaagcg cgtgcccttc agcgcgaaga
      541 cgaagccgtc cggcgtctca tcgcgccatt tgatgaagct cgccggcttc tgcaagccgt
      601 aataggtgcc gttgatctcg attgagctca gcttgcggct ggcgtattcg agctcgcgct
      661 tctgagcgag gccgtccgga tagaaggtcc cgcgccaggg ctcgtacgtc cagccgccga
      721 tgccgatgtg aatgcgcggc tttgcgcctt tgctcatcgc ccctccctgc gcctcgcggc
      781 gcttaggtct cgaaaatcgt cagcttttcc ggcgcgccgc gcagcaccgc gcggatcggc
      841 aattcctcgg ccggcccctc gccaagcgcc tttttcagca ccgcgcgttt ctcttccccc
      901 gcgatatgca gcgcaatcgc ctgggcgcga aggatgacgc gggcggtcaa agtcaggcgc
      961 ggctccgcgg cgtcgggcgc gttcatcggc aggacgagcg cccgctcgcc gggattgatc
     1021 gccgcttcga gattgtcgcc gcccggaaac cacgacgcgg tgtggccgtt atcgcccatg
     1081 ccgagcacga cgagatcggc cggcaggggc aggctggcga tgcgcgcctg cgccgcggcc
     1141 agttcctgtt cgggcgaaag ccggttgtcc gccaagggcg cgaagcgcgc ctcggcggcg
     1201 cggttttgca gcaagtgctc gcgcaccaat ttcgcgttgg agcgcgggct attctccgac
     1261 acccggcgct cgtcggcgag cgtcacattg acccggctcc agtcgatgtc ctgatgcgac
     1321 aggcgctcga aatagagttc gggcgttttg ccgcccgaga cgatgaaagt cgcctcgcca
     1381 tgcgcggcga cggcctcctg cagcagcgaa gccgaccatt ccgcaaaagc ctcggcgaga
     1441 ctttcgctcg acggaaaatc gcgccggacg atcggcgccg tttcgagatc ggcagcaaga
     1501 gcagtcattc gaattcctca ttccaagtgc gtccatcgcg ctcgatgagc gcaatcgacg
     1561 ccgcagggcc ccaagagcct gcggcatagg gccgcggcga ctcgcgcaat tgcgcccaag
     1621 cctcgcggat cggatcgatg aagcgccatg cggcctccac ctcgtcgcgc cgcatgaaca
     1681 aggtcgcgtt gccgcgcacc acgtccatca acaatcgctc gtaagcgtca atcgacaggc
     1741 ccgcaaaggc tttggcgaag ctcatatcga gcggcacgtg ctgtaaacgc atgccgcccg
     1801 cgcccggctc cttgatcatc agccataatt tgatgccttc gtccggctgg acgcggatga
     1861 cgaggcggtt cgccggtatg ccgtcggaat tggactcgaa gatggcgtgc ggcacgcgcc
     1921 tgaatccgat cacgatttcg gaaaagcgtt gcggcagccg cttgccggtg cgcaggaaaa
     1981 acggcacgcc ggcccagcgc caattggcga tgccgatttt cagcgcgacg aacgtctcgg
     2041 tgtcgctcga ttccttgccg atatcgtcca gatacgaggg aaccggcttt ccatcggcaa
     2101 ttccggcgcg atattgcccg cgcacggtga ccgccgccgc gttggcggcg ttgatcggct
     2161 cgagcgattt caggaccttg agcttttcgt cgcggagcgc atcggcgtcg agcgagcgcg
     2221 gcggctccat cgcgacgagg cacagcaatt gcagcatgtg gttctgcgtc atgtcgcgca
     2281 gcgcgccggc gccgtcataa taggcgccgc ggccttcgac gccgagcgtc tcggccaccg
     2341 tgatctggac atgatcgata tgggcggcgt tccatagcgg ctcgaacagc gcattggcga
     2401 agcgcagcgc catcagattc tgcaccgtct ccttgccgag atagtgatcg atgcggaaga
     2461 tgcggttctc cgcgaacacc gcgccgagct cgtcattgat cgcggcggcg ctcgcgccgt
     2521 ccttgccgat cggcttttcg acgatgatgc gcgcctgcgg cgtcgccatg ccattggcgg
     2581 cgagcttctt ggcgatcggg ccgaagagat cggggccagt ggcgagatag aaggcgcgca
     2641 ctttgttctc gccgtcgagc agcgctttca acctgtccca gccggcgtcg ccggtggcgt
     2701 cgaccgggac atagtcgatg cgccgaagaa accgctcgac gatcggctcg gccccgtcct
     2761 tgacgccggc gaatttccac agcgcgtctt tgacgctggc gcgaaattct tcgttgtcga
     2821 gcggacggcg cgacacgccg acaatccttg tcggatcggt gaattggccg tcggcgtcgc
     2881 gatggaacag agccggaaac aatttccgat aggcaaggtc gcccgtgccg ccgaaaacga
     2941 caagatcgaa tgggtcgact ttgatgatgc gcgaggtcat cgacgcctca gttagggcaa
     3001 attcggggag agggagccgg tgggcggctc cttaaacaac acttcgcggt cgcgcgcaaa
     3061 attggcgatc agcggcaaag ccgcgccgcc gagagcgcgc gcgtcggcgc cgatcgagcc
     3121 ggccttgatc gtcggatcga caaggccgcg gcgatcgagc gattcgtaaa tctcagcgat
     3181 gcgcgtcacc agccgcgccc gcaccgcgat cggcatcgcc ccgtcgacga tcaccgcctc
     3241 gaaatcgacc accgaaatcg ccgcgagaca ggcataggcg agggcctcgg cggtctcctc
     3301 gatccagccg tccaaatgga tggagaaatc gtcccaggcg ttgggcgtac gccagatcga
     3361 ggagggatcg acgccggcgg cgcgcaagcg ctcttcgaga cggtagatcg aagcgcaatg
     3421 aatcaattgg cgggcgacga ggcgcccgtt ttcgatccgc atgatcggaa tcggcccgag
     3481 cgctccggcc gtgccggtgc ggccggtgaa tagggcgccg tcgatgacga cgccgccgcc
     3541 gatgaacgag ccgacgaaaa agtagagaaa atcatgatga cgccagccct cgccgaaaaa
     3601 gaactcggcc gcgcaggccg acgaggcgtc attgcataga ataaccggaa ccgggcagag
     3661 cgacgcgatc tcgctcttga tgtcgacatt tcgccaaacg tccatcgcgc ccggcggcgc
     3721 gccgacctcg gcgccccagt tccagagata gaagggcgtc gccacgccga ggccggtcag
     3781 ccgggcgcgc tggcgcgacg tgaggccttg cgtcaggccg ggcagggcgc gggcgacgaa
     3841 accgagcgtc gaaccgggct gaggataggc gaaggtttcg tgcgcgcgct cgcggatcgc
     3901 ccccaaaaag tcgatcagca cgagatcgca gcttcgccgt ccgatcttca ggccgagcga
     3961 aaaagcgcct tcagggtcga gcgagaacgg cacggtcggc tgcccaacct tgccgcgctg
     4021 cggcgtctcg cgcttcaaca gcccatccgc gtgaagctga ttcataatcg ccgaagcggt
     4081 ttgaaccgaa agcccggtct gccgcgcgac ctcgattttc gagagatggc ggcgccggcg
     4141 catcaacgag aggatcagcc gctcattgta gaggcgcacg cctgtctggt cggcgccgac
     4201 gggcgccggc agccgcgtgt cgacgatttc cgtctcgccc aaggccatag ccgatctctc
     4261 ttgacggacg tccgacctga ggccacgctt gcgcctgcgt ggcggacctg ccgcgccctg
     4321 cgccgatcgc cgcaaaggcg gacaatgggc gccggcggcg ctcgtgtcaa taattcaggg
     4381 cgactgattt atcttgactc aaacagcgga ttttgcgcct atggcgctgc gacggcgcgg
     4441 gttgcagcca tactgcggct tgtgcggatc gccagg
//
LOCUS       DATGJW010000010         2445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1038741, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000010 DATGJW010000000
VERSION     DATGJW010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2445
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1038741"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VKU03_00330"
     CDS             <1..323
                     /locus_tag="VKU03_00330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HLJ69731.1"
                     /translation="RYGEGKMYTRNAGDQRVFWQGPSLGFDAGADGDRTMMLVYNLPA
                     VDAIFRRYGGIDGSAYFVGGFGFTALGGDGVIVVPIRSGVGARLGVNLSYLKFTPRAT
                     WNPF"
     gene            462..1292
                     /locus_tag="VKU03_00335"
     CDS             462..1292
                     /locus_tag="VKU03_00335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69732.1"
                     /translation="MYFVAGLLVAGLVLMLILPAFWRRALRLSARRARLLTPLSVNEA
                     IAERDQLRAEHAVARRLLERRIETLQTELAQKRVELGREMKRAALDEDKLGLTRDIAA
                     LRDELAAKARDNRALEAELGASRLALYDFSAQLDRALTQVAALRQAALKAETRADEQR
                     AAIAGLETRAAGLEARLADEARASKLKIEALEAGRGRGAEPGEGRTDPDRALREAIAK
                     LGDDVLRLAARQGEEGALLVSKLGAPPKREIVAPLPPPEKNKDKAPPLRRARTTSPAP
                     "
     gene            1350..1931
                     /locus_tag="VKU03_00340"
     CDS             1350..1931
                     /locus_tag="VKU03_00340"
                     /inference="COORDINATES: protein motif:HMM:NF025595.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4337 domain-containing protein"
                     /protein_id="HLJ69733.1"
                     /translation="MSEHVGVHVHAAHEEMVHEMAEHGGGEGLAQRVAIVTALLATIG
                     ALFGYQAGRVSTDAMLLKNDSISAQARASDMWAEYQAKSTREFLSKNLALIATDDAHK
                     AKLAAEGDRYNKEKQEISGKAKAFEEEAKAANEKAEANVLPHERMGLGVALMQAAVAL
                     ASITVLTRKTWLFYGALAFAAIGVGVSTWGYFS"
BASE COUNT          440 a          750 c          819 g          436 t
ORIGIN      
        1 tgcgctatgg cgaaggcaaa atgtatacgc gcaacgccgg cgaccagcgc gtcttctggc
       61 agggcccgtc gctcggcttc gacgccggcg ccgacggcga tcgcacgatg atgctggtct
      121 ataatctgcc ggcggtcgat gcgattttcc gccgctatgg cggcattgac ggctccgcct
      181 atttcgtcgg cggcttcggc ttcaccgctt tgggcgggga tggggtgatc gtggtgccga
      241 tccgctccgg cgtcggggcg cgactcggcg tcaatctgag ctatttgaag ttcacgccgc
      301 gcgccacctg gaaccccttc tagccggcaa attcgtctcg gaccctgacc ggcgcgcgaa
      361 tcgttagaac agcgcgcgtc ggctctttgc gcgccgttgc gcccatgcga tgcttagaaa
      421 tgttcgcgaa gcccaggtga gccgcattgg tcgagcaggc aatgtatttt gtggccgggc
      481 tcctcgtcgc cgggctcgtc ctgatgctga tcctgcccgc cttttggcgc cgggccttgc
      541 ggctctcggc gcggcgggcg cggctgctga cgccgctttc ggtcaacgag gcgatcgccg
      601 agcgcgatca attgcgggcc gagcacgccg tggcgcgccg cctcttggag cggcgcatcg
      661 agacgttgca gacggaattg gcgcaaaagc gcgtcgagct cggccgcgag atgaagcgcg
      721 ccgcgctcga tgaggacaag ctcggcctga cgcgcgacat cgccgctttg cgcgacgaac
      781 tcgccgccaa ggcgcgcgac aatcgcgcgc tggaggccga gctcggcgcg agccgtctcg
      841 cgctctacga tttcagcgcg cagctcgatc gcgcgttgac ccaggtcgcg gcattgcgcc
      901 aggcggcgct caaggcggag accagagcgg acgagcagcg cgccgcgatc gccggcctcg
      961 agacgcgcgc cgccggcctc gaagcgcgac tcgccgatga agcgcgcgct tccaagctca
     1021 agatcgaagc gcttgaagcg gggcgcggcc gcggggccga gcctggcgaa ggccgtaccg
     1081 acccggatcg cgccctgcgt gaagcgatcg ccaagctcgg cgacgatgtc ctgcgcctgg
     1141 cggcgcggca aggcgaagag ggggctttgc tggtgagcaa attgggcgcg ccgccgaagc
     1201 gcgagattgt cgcgcccttg ccgccgccgg aaaaaaacaa ggataaagcg ccgccgctgc
     1261 ggcgcgcgcg cacgacgtcg ccggcgcctt agcgcgggcg gactattccc gccttcgccg
     1321 gggggaggcg gatttcaacg gaggggcgca tgtcggagca tgttggcgtt catgtccatg
     1381 cggcgcatga ggaaatggtc catgaaatgg ccgagcatgg cggcggcgaa ggcctcgccc
     1441 agcgcgtcgc gatcgtcacg gccttgctgg cgacgatcgg cgcgctgttc ggctaccagg
     1501 ccggccgcgt ctcgaccgac gcgatgctgc tgaagaacga ttcgatctcg gcgcaagcca
     1561 gggcctccga catgtgggcg gagtaccagg ccaagagcac gcgcgaattc ctttcgaaga
     1621 atctcgcgct gatcgcgacc gacgacgcgc ataaggccaa gcttgccgcc gagggcgacc
     1681 gctacaacaa ggagaagcag gagatcagcg gcaaggccaa ggcgttcgag gaagaggcca
     1741 aggccgccaa tgagaaggcc gaggccaatg ttctgccgca cgagcggatg gggctcggcg
     1801 tcgcgctgat gcaggccgcc gtcgcgctcg cctcgatcac cgtgctgacg cgcaagacct
     1861 ggctcttcta cggcgcgctc gctttcgcgg cgatcggcgt cggcgtttcg acctgggggt
     1921 atttttcgta aagggcgaca acgccgcggc gctggatggc gcaaatttgg aaattcttgg
     1981 agcgcaaaaa tgggcgcgcc gccaaaaatt ccggttcaaa aaactgtttt ctcgtcaaaa
     2041 agcggaagac caagtttttc caaatttttc tcaggccgat ttaagcgatt ccaacgggtt
     2101 aggccgagaa aaaatttggc ggcgcgtttt tcgcgagtcc gttcgctcgt acaatgagag
     2161 gcgctggcgc cggatgataa cctaagcggg acatttgaga aagaaaatgt tcgtacctcg
     2221 ataataagct tatgacgacg tcgctatttc tccgttcgca acgatcgacc cgccccttgt
     2281 tcgactcgcc tccggcgggt cgaacaaggg gctggatgat tgtcatggcc gcctaatcgt
     2341 ccttcgcggg acttcgatta acgggtcgtg tcaacagctt acgggaaagg tacgaacaat
     2401 ttctttttca aacgctgcgt ctcgattatt atcgttggcg ccgtc
//
LOCUS       DATGJW010000011         4498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_935115, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000011 DATGJW010000000
VERSION     DATGJW010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4498
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_935115"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1026
                     /locus_tag="VKU03_00345"
     CDS             <1..1026
                     /locus_tag="VKU03_00345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HLJ69734.1"
                     /translation="IAAHATLSWPLDNIGPSLPNLMATIAGNLFELKQFSGLRILDLR
                     LPDAFAAAYPGPKFSIEGTRKLAGVEKRPLIGTIVKPSVGLTPQQTAELVETLCDADI
                     DFIKDDELQADGPSCPFAERATAVMGAINRSADRRGRKTMYAFNLTGEIDEMRRRHDL
                     VESLGGTCVMTSLNSVGLAGVIALGRHSRLPIHAHRNGWGYLTRHPALGWSYVAWQKF
                     WRLAGADHMHVNGLANKFSESDESVIKSARACLTPLFADKPCLAMPVFSSGQSAKQAA
                     GTLAALGSTDLIFAAGGGIMAHPGGPGDGVRALREAWEAAMAGVPAERYAASHPALAA
                     ALAMRSA"
     gene            1023..2414
                     /locus_tag="VKU03_00350"
     CDS             1023..2414
                     /locus_tag="VKU03_00350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="four-carbon acid sugar kinase family protein"
                     /protein_id="HLJ69735.1"
                     /translation="MSEAAELPAGLLLAFYGDDFTGSSAVMEVMTFAGLPAVMFMAPP
                     SPAQLARFSGYRAIGVASVARAQPPSWMDAHLPEAFAALAALDAPVTQYKICSTLDSS
                     PTIGSIGRAIDIGAPIFGAREGAAQWLPLVVAAPGIRRYQAFGNLFAAHAESVYRLDR
                     HPVMRAHPVTPMREADVRLHIAQQTARPIGLVDCLAMKDGSGFRRLQEELRAGRSIVA
                     LDIVDDETLRWVGAVIWRNRGKGVFAVGSQGIEYALIAHWRACGALPESRESPSARRV
                     PQIVAASGSVSANTAAQIEWAEANGFEIVALDASAAVDAREWRRAVAAAIDGARAALS
                     AGRSPLVVTARGPQDRAVAAMRAKLASGRSDPLEANARIGEGLGEVVAEVARQAKLPR
                     GAISGGDTSGFAMRALGGYALEAVAPLAPGSPLCRVFSSDSEIDGFEIALKGGQMGAP
                     DFFGSVRAGCAIS"
     gene            2427..3281
                     /locus_tag="VKU03_00355"
     CDS             2427..3281
                     /locus_tag="VKU03_00355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphogluconate dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HLJ69736.1"
                     /translation="MTKIALLGAGGKMGVRLATNLKGSRFDVDHVEVSEEARARLKET
                     VGVECMEQGQAISSADAVVMAVPDRLIGKILKTFVADLRPGAKVIMLDAAAPHAGELP
                     ERADITYFVTHPCHPPIFNDETEPQAKTDYFGGVHAKQHIVCALMQGPEAHYAECEEI
                     ARQIYKPVMRSHRCTVEQLAILEPALSETVGATFALALREATDEAVRRGVPKQAAIDF
                     IIGHLNIELAIAFGVFPEGRFSDGALQAIAEAKPVIFKDDWLERVFAPESVLKSVREI
                     CNPPSPKS"
     gene            complement(3360..3434)
                     /locus_tag="VKU03_00360"
     tRNA            complement(3360..3434)
                     /locus_tag="VKU03_00360"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3400..3402),aa:Gln,seq:ttg)
     gene            3684..4115
                     /locus_tag="VKU03_00365"
     CDS             3684..4115
                     /locus_tag="VKU03_00365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HLJ69737.1"
                     /translation="MSVAQILRDKGRDVVTTQPHQTLMEAAQLLAEKGIGAVVVSDVS
                     RRVLGILSERDIVRAIARNGAGALDQSVSKYMTQKVFTVAEDTPVDTVMETMTNGRFR
                     HVPVTTDGQLVGLVSIGDVVKHHVNEIESEHRALKDYIATA"
     gene            complement(4150..>4498)
                     /locus_tag="VKU03_00370"
     CDS             complement(4150..>4498)
                     /locus_tag="VKU03_00370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="NTE family protein rssA"
                     /protein_id="HLJ69738.1"
                     /translation="EAAAEGMESGVLGGMLGMRRGAAMLLRPFTRSESGAPGIAAVMI
                     DSFNITQDRITRSRLAGDPPDVLITARLGKIGLFEFHRAEELIALGRETARRSLDELR
                     QHIDLAPAAAGAV"
BASE COUNT          724 a         1510 c         1520 g          744 t
ORIGIN      
        1 atcgcggcgc acgccactct ctcctggccg ctcgacaata tcggcccgtc gctgcccaat
       61 ttgatggcga cgatcgccgg caatcttttc gagctcaagc agttttcggg cctgcgcatt
      121 ctcgatctgc gcctgcccga cgccttcgcc gccgcctatc ccggtccgaa attctcaatc
      181 gagggcacgc gcaagctggc cggggtggag aagcggcccc tgatcggcac catcgtcaag
      241 ccctcggtcg ggcttacgcc gcagcagacg gcggagctgg tcgagacgct gtgcgatgcc
      301 gacatcgatt tcatcaagga cgacgagctg caagccgacg gaccgagctg tcccttcgcg
      361 gagcgcgcga cggcggtgat gggcgcgatc aatcgcagcg ccgaccgtcg cggccgcaag
      421 acgatgtacg ccttcaacct gaccggcgaa atcgacgaga tgcgccgccg tcacgacctc
      481 gtcgaaagcc tcggcggaac gtgcgtgatg acgagcctca attcggtggg tctcgccggc
      541 gtgatcgcgc tcgggcggca ttccagattg ccgatccacg cccaccgcaa cggctggggc
      601 tatctgaccc gccatccggc gctcggctgg tcctatgtgg cgtggcagaa gttctggcgg
      661 ctcgccggcg cggatcacat gcacgtcaac ggactcgcca acaagttcag cgaatccgac
      721 gagagcgtca taaagtcggc gcgcgcctgt ctgacgccgc tcttcgccga caagccctgc
      781 ctggcgatgc cggtcttctc ctcgggccaa tccgccaagc aggcggccgg gaccctggcc
      841 gcgctcggat cgaccgacct catattcgcc gccggcggcg gcatcatggc ccatcccggc
      901 ggcccaggcg acggcgtccg cgcgctcagg gaggcctggg aggcggcgat ggcgggcgtt
      961 cccgccgagc gctacgcggc gagccatccc gctctcgccg cggcgctggc gatgcgctcg
     1021 gcatgagcga ggccgcggaa ctgcccgccg gcctgctgct cgccttttac ggcgacgact
     1081 tcaccggctc gtccgcggtc atggaagtga tgacctttgc cggcttgccc gcggtgatgt
     1141 tcatggcgcc acccagcccc gcgcaactgg cgcgcttctc cggctaccga gcgatcggcg
     1201 tcgctagcgt cgcccgggcg cagccgccca gctggatgga cgcgcatctg ccggaggcct
     1261 tcgccgcgct cgccgccctc gatgcgccgg tgacgcagta caagatatgc tcgacgctcg
     1321 attcctcgcc gacgatcggc tcgatcggcc gcgccatcga catcggcgcg ccgattttcg
     1381 gcgcgcgcga gggcgcggcg caatggctgc cgctcgtcgt cgccgcgccg ggaatccggc
     1441 gctatcaggc ctttggcaat ctgttcgcgg ctcatgccga gagcgtctat cggctggatc
     1501 gccatccggt gatgcgggcc catcccgtca cgccgatgcg cgaggccgac gtgcgcctgc
     1561 acatcgccca gcagacggcg cggcccatag gcctcgtcga ttgcctggcg atgaaagacg
     1621 gctcgggatt caggcggctg caagaggagt tgcgcgcggg ccgctcgatc gtcgcgctcg
     1681 acatcgtcga cgacgagacg ctgcgctggg tcggcgccgt gatttggcga aaccgcggca
     1741 agggcgtttt cgcggtgggc tcgcagggaa tcgaatatgc gctgatcgct cattggcgcg
     1801 cttgcggcgc gctgcctgaa tcccgggaga gcccgtccgc tcggcgagtg ccgcaaatcg
     1861 tcgccgcctc cggttcggtc tccgccaaca ccgccgcgca gatcgaatgg gcggaggcca
     1921 acggcttcga aatcgtcgcg ctcgacgcga gcgcggcggt cgacgcgcgc gaatggagaa
     1981 gggcggtcgc cgccgccatc gatggcgcgc gcgcggcttt gtccgccggg cggtcgccgc
     2041 tcgtcgtcac cgcccgcggt ccgcaggatc gcgccgtcgc ggcgatgcgc gcgaaacttg
     2101 cgtccggaag atctgatccg ttggaggcca acgccagaat cggcgagggg ctcggcgagg
     2161 tcgtcgccga agtcgcgcgg caggcgaaat tgccgcgcgg cgcaatttcg ggcggcgaca
     2221 cgtcgggttt cgcgatgcgc gctttgggcg gctatgcgct cgaagcggtc gcgccgctgg
     2281 cgcccggctc gcctctgtgc cgcgtatttt catccgattc cgaaatcgac ggattcgaaa
     2341 tcgcgctgaa gggcggccag atgggcgcgc ccgacttctt tggatcggtt cgcgcggggt
     2401 gcgcgattag ctagtggaga ttgaacgtga cgaagatcgc attgctcggc gccggcggaa
     2461 aaatgggcgt tcgtctcgcg accaatctga aaggctcgcg cttcgacgtc gaccatgtcg
     2521 aggtctccga ggaagcgcgg gcgcggctga aggagacggt cggcgtcgaa tgcatggagc
     2581 aaggccaggc gatctcctcg gccgacgcgg tcgtcatggc cgtgcccgat cggctgatcg
     2641 ggaaaatcct gaagacgttc gtcgccgatc tgcggcccgg cgcgaaagtc atcatgctcg
     2701 acgccgccgc gccgcatgcc ggcgaactgc cggagcgcgc ggacattact tatttcgtca
     2761 cccatccgtg ccacccgccc atcttcaacg atgaaaccga gccgcaggcc aagacggact
     2821 atttcggcgg ggttcacgcc aagcagcaca tcgtctgcgc gctgatgcaa gggcccgagg
     2881 cgcattacgc cgaatgcgaa gagatcgccc gccagatcta taagccggtg atgcgatcgc
     2941 accgctgcac ggttgagcaa ctcgccattc tcgaaccggc gctttccgag acggtcggcg
     3001 cgaccttcgc cctcgctttg cgcgaagcga cggacgaagc ggtgcggcgc ggcgtcccca
     3061 agcaggccgc gatcgatttc atcatcggcc atctcaatat cgagctcgcc atcgccttcg
     3121 gcgtctttcc ggagggccgg ttttccgacg gcgcgctgca ggcgatcgcc gaagccaagc
     3181 cggtcatctt caaggacgat tggctcgagc gggtgttcgc cccggaatcg gtgctcaagt
     3241 ctgtgcgaga gatttgcaac ccgccttcgc ccaaatcgtg acaaagggag gggagcgcgg
     3301 ggccgcggat gcagcacggc tggcgcaagc gttatgcgga ggaggcgcta atttttggct
     3361 ggctggggcg ggaggattcg aacctccgta tggcggaatc aaaatccgct gccttaccgc
     3421 ttggctacgc cccaatcgct tcgaatgttt gatccgcccc cgcgaagcgg cgcgaccata
     3481 gtgtgggacg gcgccgcccg caatccttta ccgcgcagcc gccgcagctt gtctcgcggc
     3541 gtccggccgg gactgcggtt tctgaggaat ttcaaggtct ttgccggcgc cgatcaggtc
     3601 gcctcttgcc ctggacggcg accgggcgca tccttagcgg gagcggccgc cggattgcag
     3661 gggcggcgaa gagggaggcg catatgagcg ttgcgcagat tctcagagac aagggccgcg
     3721 acgtggtcac gacccagccg catcagacct tgatggaagc cgctcagttg ctcgccgaga
     3781 aaggcatcgg cgcggtggtc gtttccgatg tcagccggcg cgtcctcggc attctctcgg
     3841 agcgcgacat cgtccgcgcc atcgcccgca acggagccgg cgcgctcgat cagtcggtgt
     3901 ccaaatacat gacccagaaa gtcttcaccg tcgccgagga cacgccggtc gatacggtga
     3961 tggagacgat gaccaacggc cgcttccgtc acgtcccggt gacgaccgac ggccaactcg
     4021 tcggcctcgt ctcgatcggc gacgtggtca agcaccatgt caacgagatc gagagcgagc
     4081 accgcgcgct caaggactac atcgccaccg cctgatgcgc cgtggggcgc ggcgggattg
     4141 ccgagccgct cagaccgcgc cggcggcggc cggggcaagg tcgatatgct ggcgcaactc
     4201 gtcgagcgag cgccgcgccg tctcgcggcc gagcgcgatc agctcttcgg cgcgatggaa
     4261 ttcgaacagg ccgattttgc cgaggcgggc ggtgatcaag acgtccggcg gatcgccggc
     4321 gagccgcgac cgggtgatcc ggtcttgcgt aatgttgaag gagtcgatca tcacggcggc
     4381 gatgcccggc gcgccgctct ccgatcgggt gaaaggacgc agcaacatcg ccgcgccgcg
     4441 tcgcatcccg agcatgccgc cgaggacgcc gctctccatg ccttcggccg cggcttcc
//
LOCUS       DATGJW010000012         2686 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_623907, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000012 DATGJW010000000
VERSION     DATGJW010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2686)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2686)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2686
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_623907"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1156
                     /locus_tag="VKU03_00375"
     CDS             <1..1156
                     /locus_tag="VKU03_00375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="topoisomerase C-terminal repeat-containing
                     protein"
                     /protein_id="HLJ69739.1"
                     /translation="RDFWKDFSAAIGGTKELRTAQILDSLNELLGPHIFPPRADGADP
                     RLCPNCNKGQLSLKLGKYGAFIGCSNYPECRYTRTLAQSANGEAQADGERAGVRVLGE
                     DPQTGEEISVREGRFGAYLQQGEGDKPKRSSLPKDLAPADVTLAQAIALLSLPREVAR
                     HPTTGEPILAGLGRYGAYVQHGKTYANLGKDDHILEIGANRAIDLIVAKESGVGGRRF
                     GPSPGRVLGEHPTLKGPVAVKEGRYGAYVNHGKVNATLPQGVDPATISLDSAVALLAD
                     KASGRAGGGRPLGEHPSGGPVTVRAGRFGAYVNWGRVNATIPKSLSPDAVTLGEALDL
                     IAEKGGRPAAKGKAKPPATSAAKAKAKPGRAPQKAAKPAAKTAEKARRKA"
     gene            1308..1976
                     /locus_tag="VKU03_00380"
     CDS             1308..1976
                     /locus_tag="VKU03_00380"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLJ69740.1"
                     /translation="MAKQSAARPFAAPLSSDGLYGRFAPLYDLVFDLPFHPGRAAAAR
                     AAAEAAGATGEVLVVGVGTGLELGLLPKTLRVTGIDLSARMLDIARDRVARKALRQVK
                     ALQVMDAAALEFPAARFDVALAPYVMSVVPSPTRALDEMWRVVKPGGEIVIVNHFSSE
                     RGLRVRIEQAMEQSASWLGWHPVFPYASIGEWVAGRKEAQVVEKRQVAPFRMFTLLRV
                     KKTA"
     gene            1987..2352
                     /locus_tag="VKU03_00385"
     CDS             1987..2352
                     /locus_tag="VKU03_00385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69741.1"
                     /translation="MGRIKLFLCNTLIAFLYFIYALGGGAAAEENYDLFKYGGLSCDE
                     WIRHSTLTEAETPKWFLGDLKQLYESGGYRLDILNQFKPDQITAWLDSYCRARPLDGL
                     AVAGTVLTNDLAERAAAKK"
BASE COUNT          464 a          925 c          861 g          436 t
ORIGIN      
        1 gcgcgatttc tggaaggact tttccgccgc gatcggcggc accaaggaac tgcgcaccgc
       61 gcagatcctc gacagtctga acgaactgct cggcccgcac attttcccgc cgcgcgccga
      121 cggcgccgat ccgcgccttt gtccgaattg caacaagggc cagctttcgc tgaagctcgg
      181 caaatacggc gcttttatcg gctgctcgaa ctatcccgaa tgccgctaca cgcgcaccct
      241 cgcgcagagc gccaatggcg aggcgcaagc cgacggcgag cgggccggcg tgcgcgtgct
      301 cggcgaggac ccgcaaacgg gcgaagaaat ctcagtccgc gaggggcgct tcggcgccta
      361 tctgcagcag ggcgagggcg acaagccgaa acgctcgtcg ctgcccaaag atctcgcccc
      421 ggccgacgtg accctggcgc aggccatcgc cctgctctcg ctgccgcggg aagtggcgcg
      481 ccacccgacc accggcgagc cgatccttgc cggcctcggc cgctacggcg cctatgtgca
      541 gcacggcaag acctacgcca atctcggcaa ggacgaccac atcctggaga tcggcgccaa
      601 ccgcgcgatc gacctcatcg tcgccaagga aagcggcgtc ggcgggcggc gcttcggccc
      661 gtcgcccggc cgcgttctcg gcgagcatcc gacgctgaaa ggcccggtcg cggtcaagga
      721 aggccgctac ggcgcttacg tcaatcacgg caaggtcaac gcgactttgc cgcaaggggt
      781 cgatcccgcg acgatctcgc ttgattccgc agtcgccctg cttgccgaca aggcctcggg
      841 ccgcgccggc ggcgggcggc cgctcggcga gcatccgtca ggcggtccgg tcaccgttcg
      901 cgccggccgc ttcggcgctt atgtcaattg gggcagggtc aacgcgacga tccccaaatc
      961 cttgtcgccg gacgcggtca cgctcggcga agcgctcgac ttgatcgcgg aaaagggagg
     1021 ccggccggct gcgaagggca aggccaagcc gccggcgaca tccgccgcca aggccaaggc
     1081 gaagcccggc cgcgcgccgc agaaagcggc caagccggcg gccaaaaccg ccgagaaggc
     1141 gcggcgaaaa gcctaaagca cgcttaaggg cgcgcgcctc atgtgaacaa tcgcgccttt
     1201 ttctcgttct tagcgggttc aatcagccgc gccttgcatg tccggccgcc ttgcgccaat
     1261 tttgagcgcg ccgggcggcg cggccccggc gcggagggac gaagctcatg gccaagcaga
     1321 gcgcggcgcg gcccttcgcc gctccgcttt cctccgacgg cctctatggc cgctttgcgc
     1381 ccctctacga cctggttttc gatctgccct tccatcccgg ccgggccgcg gcggcgcgcg
     1441 ccgcggccga ggccgccggc gcgaccggag aagtgctcgt cgtcggcgtc ggcacgggac
     1501 tcgaactcgg cctcctgccc aagacgttgc gggtgacggg catcgatctt tccgcgcgca
     1561 tgctcgacat cgcccgcgac cgagtcgcgc gcaaagcctt gcgccaggtc aaggctttgc
     1621 aggtgatgga cgccgcggcg ctcgaatttc cggcggcgcg cttcgacgtc gcgctcgccc
     1681 cttacgtgat gagcgtcgtg ccgtcgccga cccgggcgct cgacgagatg tggcgggtcg
     1741 tcaagccagg cggcgaaatc gtcatcgtca atcacttctc gtccgagcgc ggtctgcgcg
     1801 tgcggatcga gcaagcgatg gagcagtcgg cctcttggct cggctggcat ccggtcttcc
     1861 cttacgcttc tatcggcgaa tgggtcgccg ggcgcaagga agcgcaggtg gtcgaaaagc
     1921 gccaagtcgc gccgttccgg atgttcacgc ttctgagggt gaagaagacg gcgtagcgag
     1981 gataagatgg ggcggataaa gcttttcctt tgcaatactt tgatcgcgtt tctttacttt
     2041 atttatgcgc ttggcggcgg cgcggcggcc gaagagaatt acgatctctt caaatatggc
     2101 ggcctttcct gcgacgaatg gatccgccat tccacgctga ccgaagcgga gacgccgaaa
     2161 tggttcttag gcgatctcaa gcagctttac gaaagcggcg gctatcgcct tgatattctg
     2221 aaccagttca agcccgatca aatcacagcc tggcttgatt cctattgccg cgcgcggccc
     2281 ctcgacggcc tcgccgtcgc cggaaccgtg ctgaccaacg acttggccga gcgcgccgca
     2341 gcgaagaaat agcggcggtg caatgcgcga agcgcggccg gatgaggggc cgcgctgagc
     2401 ccggtgattg gcgtttcatg gagcgctcac cttcgcgttc cgcgccgccc ctcatccggc
     2461 gcttcgcgcc accttctccc cgtcttgcgg ggagaaggga tttcccggcc ttgcgcaaga
     2521 cgcctcgcgt catgatatag gctctatccg cggcgctggc gccgccgatt ccaaaaacat
     2581 ttcccaaacg aagtcgaatt gcccaaaacc atgaaaaagg cgcgccccga gcgcgcaaga
     2641 ccgccgcgcg acaaacgcgc gccccacgaa gccgccctgc ccacgc
//
LOCUS       DATGJW010000013         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1454167, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000013 DATGJW010000000
VERSION     DATGJW010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1454167"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..441
                     /locus_tag="VKU03_00390"
     CDS             <1..441
                     /locus_tag="VKU03_00390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="insulinase family protein"
                     /protein_id="HLJ69742.1"
                     /translation="SLLYRVLTVEERIAAAIGAQYMGSALDDTRFFLYALPAPGVTLD
                     ALEKAVDRVIAQFLVEGVDEPTLERAKTRLIADAIYAQDSQAMLARWYGASLSTGLGI
                     DDVKQWPARIEAVDAAAVRAAAKKHLDARKAVSGFLLPEEKAAA"
     gene            683..1981
                     /locus_tag="VKU03_00395"
     CDS             683..1981
                     /locus_tag="VKU03_00395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLJ69743.1"
                     /translation="MTALVHQQTVSRAVPVQQIVSPRGVKAWLVEDYAVPLVSIEFAL
                     KGGAAQDPPGKAGAATLLAGLLDEGAGPLDSQAFHRALDEKAVEISFSSDRDHISGRM
                     RSLIRNLDRAAELLRLAVNEPRFDEEPFVRVREHFNATLRREAKDPSSLAWRTWRART
                     FPNHPYGAPARGTLESLAAMERADIVALAKRLIARGALHVAIVGALDAARATKLLDHV
                     FADLAPESVLTPVADIEPAGLGGIEIVDLDVPQSTIRYGRPGIKRHDPDRMTAIVLTH
                     ILGGGTGLSSRLFREVREKRGLAYSVDASLAALDHAAFLHGGTSTKNERAKESLDVIR
                     NEILDLAESGPNEDELEKGKKYLVGSYALRFDTSAKIAGQLVHIQMDGYGPEWLVERN
                     RQIEAVTAAEAKRVARRLFGDGALSVVAVGRPAGLHVPTA"
BASE COUNT          336 a          690 c          674 g          322 t
ORIGIN      
        1 agcctgctct atcgcgttct gaccgtcgag gagcgcatcg cggcggcgat cggcgcgcaa
       61 tatatgggct cggccttgga cgatacgcgc ttcttcctct acgccttgcc ggcgcccggc
      121 gtgactttgg acgctctcga aaaagccgtc gatcgcgtga tcgcgcaatt tctcgtcgaa
      181 ggcgtcgacg agccgacgct ggagcgcgcc aagactcggc tgatcgccga cgcgatctac
      241 gcccaggaca gccaagcgat gctggcgcgc tggtacggcg cttccctctc gaccgggctc
      301 ggcatcgacg acgtgaagca atggccggcg cggatcgaag ccgtcgacgc cgccgcggtg
      361 cgcgccgcgg cgaagaagca tctcgacgcg agaaaagcgg tcagcggctt cctcctgccg
      421 gaagaaaagg cggcggctta ggcaggctgc tttggccggc gcatgcagct taccctcacc
      481 cccagcccgc aagcgggaga gggggcgcgg ggccagatcg cggggaatcc cttctcccgc
      541 ttgcggggag aaggtggcgc gaagcgccgg atgagggcga gccgcggcgc tgacgatcgg
      601 cgcgcgccgc gccgcatcgg gagagaaaca ggctttgacc gcccttggcg gcaaggctca
      661 agtccttgaa aggcgagaat tcatgaccgc actcgtccat caacagaccg tctcgcgcgc
      721 cgtgccggtg cagcagatcg tctcgccgcg cggcgtcaag gcctggctgg tcgaagacta
      781 cgccgtgccg ctggtctcga tcgaattcgc gctgaagggc ggcgcggcgc aggatccgcc
      841 cggcaaagcg ggcgccgcga cgctgctcgc cggccttctc gacgagggcg ccggcccgct
      901 cgattcgcaa gccttccacc gcgcgctcga cgagaaggcg gtcgaaatct cgttcagttc
      961 tgaccgcgac catatcagcg gccgcatgcg ctcgcttatc cgcaatctcg accgcgccgc
     1021 cgaattgctg cgcctcgcgg tcaacgagcc gcgctttgac gaagagccgt tcgtccgcgt
     1081 gcgcgagcat ttcaacgcga cgctgcgccg cgaagccaag gacccgtctt cgctcgcctg
     1141 gcggacctgg cgcgcccgca cctttccgaa tcatccttat ggcgcgccgg cgcggggaac
     1201 attggaaagc ctcgccgcaa tggagcgcgc cgacattgtc gcgctcgcca agcggctgat
     1261 cgcgcgcggg gccttgcatg tcgcgatcgt cggcgcgctc gacgcggcgc gcgcgacgaa
     1321 gctgctcgac catgtcttcg ccgatcttgc gccggaaagc gtcctgacgc cggtcgccga
     1381 tatcgagccg gccggccttg gcggcatcga gatcgtcgat ctcgacgtgc cgcaatcgac
     1441 gatccgctac ggccggccgg ggatcaaacg ccacgatccc gaccgcatga ccgccatcgt
     1501 cctcacccat attctcggcg gcggcaccgg cctgtcgtca cggctgttcc gcgaagtgcg
     1561 cgagaagcgc ggcctcgctt attcggtcga cgcgtcgctg gcggcgctcg atcacgccgc
     1621 cttcctgcat ggtggcacct cgaccaagaa cgagcgggcg aaggaatcgc tcgacgtgat
     1681 cagaaacgag attctcgacc tcgccgaatc cggccccaac gaggacgagc tcgaaaaggg
     1741 caagaaatac ctggtcggct cctacgcttt gcgcttcgac acgtcggcca agatcgccgg
     1801 ccagctcgtc cacatccaga tggacggcta tgggccggaa tggctcgtcg agcgcaatcg
     1861 ccaaattgaa gcggtgaccg cggcggaagc caagcgcgtc gcccgccgcc tgtttggcga
     1921 cggcgccctg agcgttgtgg cggtgggacg gccggcgggg ctgcacgtcc cgacggcctg
     1981 atcggcggcc tatttgtagt tttgcgggtc ggccatgcac tt
//
LOCUS       DATGJW010000014         5973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1039047, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000014 DATGJW010000000
VERSION     DATGJW010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5973
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1039047"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1385)
                     /locus_tag="VKU03_00400"
     CDS             complement(<1..1385)
                     /locus_tag="VKU03_00400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLJ69744.1"
                     /translation="MTIEGPLPNRLSQPFEDAAEIQAVLGPTNTGKTHYAIDRMLAHR
                     SGVIGLPLRLLAREVYGRIAERAGAASVALITGEERINPGDPRYWVCTVEAMPRGLKP
                     DFLAVDEIQLAADLQRGHTFTDRLLNYRGRLETLFLGAATIEPVIRTLAPKAATSTRP
                     RLSSLVHEGERKLTRLPRRSAIVAFSAQDVYEIAELIRRRHGGAAVVLGALSPRTRNA
                     QVDLYQNGDVDYIVATDAIGMGLNLDIEHVAFASDQKFDGREYRRLTFAEFAQIAGRA
                     GRNRRDGSFGSTGRCPPFEPDLVEALETHEFAPAARALWRNSELDFSSIEALLQTLRR
                     FPERAGLMRSDSGDDIRALEILSGDPAVRRAAKTPADIGRLWDACQIPDYRNVSGGAH
                     AELVKSVFEFIVRKGNISEDWFWRHLSSADRTEGDLDALSSRIDQVRSCAYMANREDW
                     LADPGHWQVIAR"
     gene            1694..2299
                     /gene="leuD"
                     /locus_tag="VKU03_00405"
     CDS             1694..2299
                     /gene="leuD"
                     /locus_tag="VKU03_00405"
                     /EC_number="4.2.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009316 - 3-isopropylmalate dehydratase
                     complex [Evidence IEA];
                     GO_function: GO:0003861 - 3-isopropylmalate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009098 - leucine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-isopropylmalate dehydratase small subunit"
                     /protein_id="HLJ69745.1"
                     /translation="MDKFTTLTGVAAPLPIDNIDTDMIIPKQYLKTIKRTGLGKGLFA
                     EMRYKDDGSPNPDFVLNKPAYAKAQIIVAGDNFGCGSSREHAPWALLDFGIRCVISTD
                     FADIFYNNCFKNGILPIKVSAEDLAKLMDDAERGANATLSIDLEKQEIRGPDGGLVRF
                     DIDPFRKHCLLEGLDDIGLTMVEAPAVERYEKKAAAAHPWI"
     gene            2665..3846
                     /locus_tag="VKU03_00410"
     CDS             2665..3846
                     /locus_tag="VKU03_00410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HLJ69746.1"
                     /translation="MASARLLLAILAASLAPPGAARADFASCLAAIRNEAVHSGIRGS
                     VFDQATAGVQPDMKILGLQKNQPEFKTPIWDYLAGLVDDERIADGKDGLRKYARAFAL
                     AEERYGVPRTIIAAVWGVESNYGKTMGARPLIQSLATLGCYGERAGYFRGELIAALKI
                     VDHGDIAADRLVGSWAGAFGQTQFMPSTFLRLAVDLDGDGRRDIVDSVPDAIGSTANF
                     LHRAGWRSGLGWGFEVKLPEGYSGPSGRRRKAPMSAWAARGIRRADGRPLGEGEAGLL
                     LPAGPNGPAFLVTSNFDAIYSYNAAESYALAIAHLSDRLAGGGPFVTPWPTDDPGLSR
                     AERRELQRLLIRRGYDVGEPDGAIGDKTREAIADFQRKIGRPPDGRASVKVLAALRQG
                     M"
     gene            complement(3871..4266)
                     /locus_tag="VKU03_00415"
     CDS             complement(3871..4266)
                     /locus_tag="VKU03_00415"
                     /inference="COORDINATES: protein motif:HMM:NF017020.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEPN domain-containing protein"
                     /protein_id="HLJ69747.1"
                     /translation="MTPEAARFLDKAQKLLAEADVMIGVGLTDAAGRTAYLAAFHAAQ
                     ALIFERIGRTLKSHTGVHAEFLRLTKDDSNIGELRAFLSRAYNLKALADYETGPGSEI
                     SPDRATKALADAKLFVAYLAALIERSGPK"
     gene            complement(4263..4583)
                     /locus_tag="VKU03_00420"
     CDS             complement(4263..4583)
                     /locus_tag="VKU03_00420"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLJ69748.1"
                     /translation="MGETIDDPVLARFRTALEDIYGDRLDRVVLFGSRARGDAGPDSD
                     YDIAVFLTSLPDRWSELDRLADLRVRFLDDSGVFFDVRPYSAETYRDATPLMSEIRHD
                     GLRL"
     gene            complement(4908..5669)
                     /gene="hisF"
                     /locus_tag="VKU03_00425"
     CDS             complement(4908..5669)
                     /gene="hisF"
                     /locus_tag="VKU03_00425"
                     /EC_number="4.3.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011508734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000107 - imidazoleglycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazole glycerol phosphate synthase subunit
                     HisF"
                     /protein_id="HLJ69749.1"
                     /translation="MLKVRVIPCLDVKDGRVVKGVNFVGLRDAGDPVQCAIAYDAAGA
                     DELCFLDITASHENRGILLDVVRRTAEACFMPLTVGGGVRTIDDIRNLLLAGADKVSI
                     NTAAVKDRGFVKAAAQKFGSQLVVVAVDAKKVGPGRWEIFTHGGRNATGIDAIDYARD
                     VVALGAGEILLTSMDRDGAKSGYDLELTRAVADGVNVPVIASGGVGTLDHLVDGVRKG
                     HASAVLAASIFHFGEHTIREAKAHMAAAGLAMRMD"
     gene            complement(5662..>5973)
                     /locus_tag="VKU03_00430"
     CDS             complement(5662..>5973)
                     /locus_tag="VKU03_00430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HLJ69750.1"
                     /translation="DLSALELGRRFEDAGVGAIIYTDIARDGALKGLNIEATLALAEA
                     VSIPVIASGGLASLDDVARLTEPDCARLAGAIAGRALYDGRLDPAQALALIRKGRGAH
                     A"
BASE COUNT         1101 a         1936 c         1922 g         1014 t
ORIGIN      
        1 cgcgctatga cctgccaatg tcccggatcg gcgagccaat cttcgcgatt ggccatatag
       61 gcgcaggagc ggacctggtc gatgcgcgac gacaacgcgt cgaggtcgcc ctcggtccgg
      121 tcggcgctgg acaaatggcg ccagaaccag tcctcggaaa tattgccctt gcgcacgatg
      181 aattcaaaca ccgatttgac cagctcggca tgggcgccgc cggagacgtt gcggtaatcg
      241 ggaatctggc aggcgtccca cagccggccg atatcggccg gcgtcttggc ggcgcggcgc
      301 acggcgggat cgccggagag aatctccagc gccctgatgt cgtcgccgga atccgagcgc
      361 atcaggccgg cccgctccgg gaagcggcgc agcgtctgca gcagggcctc gatcgacgag
      421 aaatcgagct cgctgttgcg ccacagcgcc cgcgccgccg gcgcgaattc atgggtttcg
      481 agcgcctcga ccaggtccgg ttcgaaaggc gggcagcgcc cggtcgagcc gaacgagccg
      541 tcgcggcggt tgcggccggc ccggccggcg atctgggcga attcggcgaa ggtcagccgc
      601 cgatattcgc ggccgtcgaa tttctggtcg gaggcgaagg cgacatgctc gatgtcgaga
      661 ttgagtccca tgccgatcgc gtcggtggcg acgatatagt cgacatcgcc gttctggtag
      721 agatcgacct gagcgttgcg ggtgcgcggc gacagcgcgc cgagcacgac cgcggcgccg
      781 ccatggcggc ggcggatcag ctccgcgatc tcgtagacgt cctgcgccga gaaggcgacg
      841 atcgccgagc gccgcggcag ccgggtcagc tttctttcgc cttcatggac gagcgaggaa
      901 aggcgcggcc gcgtcgaggt cgccgctttc ggcgccagcg tccggatgac cggctcgatc
      961 gtcgccgcgc cgagaaacag cgtctccagc cggccgcgat aattgagcag gcgatcggtg
     1021 aatgtgtggc cgcgctgcaa atcggcggcg agctggatct cgtcgacggc gagaaaatcg
     1081 ggcttaaggc cgcgcggcat cgcttcgacc gtgcagaccc agtaacgcgg atcgcccgga
     1141 ttgatccgct cctcgccggt gatcaaagcg accgaggccg cgcccgcccg ctcggcgatg
     1201 cggccataga cctcgcgcgc cagcagccgc agcggcaggc cgatcacccc ggagcgatgg
     1261 gcgagcatcc ggtcgatcgc gtaatgcgtc ttgccggtat tggtcgggcc caggaccgct
     1321 tggatttcgg cagcgtcttc aaagggctga ctgaggcgat tgggcaaagg cccttcaatc
     1381 gtcatgaggc ttttatgtcc tgaatgccgc cgcggcgccg cggaaatgaa gaaagtcttg
     1441 ccgccggcag cggttaaatc cgcgccgaaa gcggcgattc gaacgggatg ggaacgaatc
     1501 gcggccgaat cggcgactcg tcgggaatcc ggttttgttc acgacaatct atggatggtt
     1561 ttgcgagagt cccgccacat ttagaatcgc gccgcgctgc gacggttgat cgaggtccgg
     1621 catttgcgcc caaataggac atttgttaaa tcgccgccac gcgccgcctc acgcggcctt
     1681 catgagaact tcgatggaca agttcacgac cctgaccggc gtcgccgcgc cgctgccgat
     1741 cgacaatatc gatacggata tgatcatccc caagcaatat ctcaagacga tcaagcggac
     1801 cgggctcggc aagggcctct tcgccgaaat gcgctacaag gacgacggct cgcccaatcc
     1861 cgatttcgtg ctcaacaagc ctgcttacgc caaggcgcaa atcatcgtcg ccggcgacaa
     1921 tttcggctgc ggctcgtcgc gcgagcacgc gccctgggcg ctgctcgatt tcggcattcg
     1981 ctgcgtgatc tcgacggatt tcgccgacat tttctacaat aactgcttca agaacggcat
     2041 cctgccgatc aaggtctcgg ccgaggatct ggccaagctg atggacgacg ccgagcgcgg
     2101 cgccaacgca acgctgagca tcgatctgga aaagcaggaa atccgcggcc ccgacggcgg
     2161 cctggtgcgc ttcgacatcg acccgttccg caagcattgc ctgctcgaag gcctcgacga
     2221 tatcggcctc accatggtcg aagcgccggc ggtcgaacgt tacgagaaaa aagccgccgc
     2281 ggcgcatcct tggatttgaa cggaaccaat ttggcgggcc agacagcggc gcagcgggct
     2341 accctcatcc ggcccttcgg gccaccttct cccgcaagcg ggagaaggga ttagcgccct
     2401 tttctgacga ccttgcagag cgccgtataa cgcgcccggc gcgccccctc tcccgcttgc
     2461 gggagagggt tggggtgagg gtgaaccgcc gcttttgctc aatgaaacaa acaggctcga
     2521 cccccagtcg cgaatcgccg atgatggaga ctgtgagaac atcgccccag ttactgcgag
     2581 cgaagccaag ccctccactc gaagggcggc gcccttcgag tggagggctt cgcttcgccc
     2641 gcgatgacga ttagggcccc gccaatggcg agcgcccgcc ttctcctcgc tatcctcgcc
     2701 gcctcgctcg cgccgcccgg cgccgcgcgg gcggatttcg cctcttgtct cgccgcgatt
     2761 cgcaacgagg ccgtccatag cggaatccgg ggctcggtct tcgaccaggc gaccgccggc
     2821 gtgcagccgg acatgaaaat cctcggcctg cagaagaacc agccggaatt caagacgccg
     2881 atctgggact atctcgccgg cctcgtcgac gacgagcgga tcgccgacgg caaggacggc
     2941 ttgcgcaaat acgcccgcgc cttcgctttg gcggaggagc gctacggcgt tccgcgcacg
     3001 atcatcgccg cggtctgggg cgtcgaatcc aattatggca agacgatggg cgcgcgcccg
     3061 ctgatccaat cgctggcgac gctcggctgc tacggcgagc gcgccggcta ctttcgcggc
     3121 gagttgatcg ccgctttgaa gatcgtcgac catggcgaca tcgccgccga tcgcctcgtc
     3181 ggctcctggg ccggcgcctt cggccagacc cagttcatgc cctcgacctt tctacgtctc
     3241 gccgtcgatc tcgacggcga cggcaggcgc gacatcgtcg attccgtacc cgatgcgatc
     3301 ggctcgaccg ccaatttcct gcatcgcgcc ggctggcgct cagggctcgg ctggggcttc
     3361 gaagtcaaac tgccggaagg ctattcgggc ccgtccggcc ggcggcgcaa ggcgccgatg
     3421 tcggcttggg cggcgcgcgg aatcaggcgc gccgacgggc gtccgctcgg cgaaggcgag
     3481 gccggtcttt tgttgccggc cggaccgaac gggccggcct tcctcgtcac ctcgaatttc
     3541 gatgcgatct attcctacaa cgccgccgag tcctatgcgc tcgccatcgc ccatctctcc
     3601 gatcgcctcg ccggcggcgg cccgttcgtc acgccttggc cgaccgacga tcccggcctg
     3661 tcgcgcgccg agcggcgcga attgcagcgg ctcctgatca ggcgcggcta cgatgtcggc
     3721 gagcccgacg gcgcgatcgg cgacaagacg cgcgaggcga tcgccgattt ccagcgcaag
     3781 atcggccgcc cgccagacgg cagagcatcg gtgaaagtgc tggcggcgct caggcaggga
     3841 atgtaagtcg cgagaccggt cgtgcgaact ctattttggc cctgagcgtt cgatgagggc
     3901 ggccagatag gcgacgaaca gtttggcgtc agcaagagcc tttgtcgcgc gatcgggcga
     3961 aatttcggag cctggccccg tctcgtagtc agcgagagcc ttgaggttgt aggcgcgcga
     4021 caagaaggcg cgcaactcgc cgatattaga atcgtctttg gtgagacgga ggaattcggc
     4081 atgaaccccg gtgtggcttt tcaaagttct tccgattcgc tcaaagataa gcgcttgggc
     4141 ggcatggaag gcggcaagat aggcagtacg tccggcggcg tctgtgagac cgacgccgat
     4201 catcacatcg gcttccgcca ggagcttttg cgctttatcc aaaaatcgag cggcctctgg
     4261 cgtcacaatc gaagtccatc atgacgaatt tcgctcatga gcggcgttgc gtcccgatag
     4321 gtctcggcag aatagggtcg aacatcgaag aagacgcccg aatcatcgag aaagcgaacc
     4381 cgcaaatcgg caagacgatc cagttcgctc cagcggtcgg gcaacgaggt caagaagacg
     4441 gcgatatcgt aatcggaatc cggccctgca tcgcctcgcg cgcgcgatcc gaacagcacg
     4501 actcggtcga gccgatcgcc gtaaatgtcc tcaagcgccg tgcggaagcg cgccaagacc
     4561 gggtcgtcaa tcgtctcgcc cataggcagc tcatcggagg aaccgagcaa aggttaccac
     4621 attacggctg ggaacgacgt gattcctcgt ttagaaagag tttcgcctcc gcgtcagtag
     4681 cgaactttcg tgcgctcaca agcagcccat cgacccaaat ttcgacgtga taaggagtcg
     4741 gttgtgggcg tccagcccaa atagcttcct cgccccaaat gctctcccat atgctttccg
     4801 ttaagcatag cagaaaatga gagcatagcg agccgccgtc aaaaggatcc aaactcaaac
     4861 ttggcgctat ccgagcttcg cgggcgtcct ccagaccgga agaacgttca atccatccgc
     4921 atcgccagac ccgccgccgc catatgcgcc ttggcttcgc ggatcgtgtg ctcgccgaaa
     4981 tggaagatcg aggcggcgag gacggcgctg gcgtggcctt tcctcacgcc gtcgacgaga
     5041 tgatcgagcg ttccgacgcc gcctgaagcg atgaccggca cattgacgcc gtcggcgacg
     5101 gcgcgggtca attccagatc gtagcctgac ttggcgccgt cgcgatccat cgaggtcagc
     5161 aggatttcgc cggcgccgag cgcgacgacg tcgcgcgcat agtcgatcgc gtcgatgccg
     5221 gtggcgttgc ggccgccatg agtgaaaatc tcccaccggc ccggcccgac ctttttggcg
     5281 tcgaccgcga cgacgaccag ttggctgccg aacttctgcg ccgccgcctt gacgaagccg
     5341 cgatccttca ccgcggcggt gttgatcgag actttgtcgg cgccggcaag cagcaggttg
     5401 cggatatcgt cgatcgtgcg cacgccgccg ccgaccgtca gcggcataaa gcaggcttcg
     5461 gccgtgcggc gcacgacatc gagcaggatg ccgcgattct catggctcgc cgtgatgtcg
     5521 agaaagcaga gctcgtcggc gccggccgcg tcataggcga tcgcgcattg caccgggtcg
     5581 ccggcgtcgc gcaggccgac gaagttcacg cccttgacga cgcggccgtc cttcacgtcg
     5641 aggcagggga tgacgcggac cttaagcatg cgcgcctcgt cctttgcgga tgagcgccaa
     5701 agcctgggcc gggtcgagcc ttccgtcata gagcgcgcgc ccggcgatcg cgccggcgag
     5761 gcgcgcgcaa tcgggctcgg tcaagcgcgc cacgtcgtcg agcgaggcaa ggccgcccga
     5821 ggcgatgacc ggaatcgaaa ccgcttccgc caaggcgagc gtcgcctcga tgttgaggcc
     5881 cttgagcgcg ccgtcccgcg cgatgtcggt atagatgata gccccgacgc ccgcgtcctc
     5941 gaagcgccgg ccaagctcga gcgcggagag atc
//
LOCUS       DATGJW010000015         3600 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_105839, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000015 DATGJW010000000
VERSION     DATGJW010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3600)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3600)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3600
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105839"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..566)
                     /locus_tag="VKU03_00435"
     CDS             complement(<1..566)
                     /locus_tag="VKU03_00435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012042982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLJ69751.1"
                     /translation="MFTSSIEVLDERFEKLAHGNVHLEKLYTGCRWAEGPAYFAAGKY
                     LVWSDIPNDRVMRYDDTDGSVSVFLAPAMNHNGHTVDREGRLVSCEHRGRCVSRIEHD
                     GRRVVLADRFEGKRLNSPNDVVVKSDGSIWFTDPPFGILGNYEGDRADPELTPAVYRV
                     DGETGEATVVADDIQGPNGLCFSPDEKRL"
     gene            complement(663..1481)
                     /locus_tag="VKU03_00440"
     CDS             complement(663..1481)
                     /locus_tag="VKU03_00440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EcsC family protein"
                     /protein_id="HLJ69752.1"
                     /translation="MTLEIMTEELSEADLNELRAAVRALEGDSFATRMADLIGRQVQA
                     AGRALPSPARKVIARASERALRTALRLAIKTIDPNAPAKPQVGLHKALAAASGAAGGA
                     FGLAALAAELPVSTTIMLRSIAQIARAEGEDLSAPEAALACIEVFALGGHAPGEAALE
                     SEYFAARGALAISLRDSARFILHHGLANETAPVLVRLVSQIAARFGVVVSEKLAAQAL
                     PVVGAVGGAAVNLAFVQHFQTIARGHFTVRRLERAHGAELVRFEYERLRNEKTR"
     gene            complement(1629..2318)
                     /locus_tag="VKU03_00445"
     CDS             complement(1629..2318)
                     /locus_tag="VKU03_00445"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLJ69753.1"
                     /translation="MQAVSGPTTPVASGRFVLRTTEKRKGFAHLMHLFGNLGHVVILG
                     PLALATLFYLIAIGARRDAAAFAIALLAGLAATIGAKLFFSLCGLHVLHPRMESPSGH
                     ESFSAAVYGCIALIVATGRPKLQQVLIGGAAALLIVLIGIGRVAQHAHSPQEVAFGLS
                     IGIAATALFHLLRGAAARPISLPWRAMALLSPAALVLAFGALVFLRHWTPEYFIEGIG
                     RRLGAHFGLCL"
     gene            2372..3052
                     /locus_tag="VKU03_00450"
     CDS             2372..3052
                     /locus_tag="VKU03_00450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69754.1"
                     /translation="MFDPWIRLARAATPDGDELILRRRGGEFEIRFNGWELMASRTSR
                     SEEALARLAFQELRRPPRAVLIGGLGMGYTLRAALDLAPPDARIVVSELVPAIVEWAR
                     GPLAALAGRPLDDPRVEIRVGGVADSLAASRHAFDAVLLDTDNGPEAVLREANGFLYS
                     REGLESAIGAVAGDGAIAFWSADRSEKFEDVLNAADVQWRRLDVEARRGGPAHTIYLA
                     RPPAKARG"
BASE COUNT          597 a         1191 c         1254 g          558 t
ORIGIN      
        1 agccgcttct cgtcgggcga gaagcacagc ccgttcggcc cctgaatgtc gtcggcgacg
       61 accgttgcct cgccggtctc gccgtcgaca cggtagacgg cgggcgtcag ttccgggtcg
      121 gcccggtcgc cctcgtaatt gccgaggatg ccgaacggcg ggtcggtgaa ccagatcgaa
      181 ccgtccgact tgacgacgac gtcgttcggc gaattgagcc gcttgccctc gaatcgatcg
      241 gcgagaacga cgcgcctgcc gtcgtgctcg atgcgcgaga cgcagcggcc gcgatgctcg
      301 caggagacga gccgtccctc gcggtcgacg gtatggccat tgtgattcat cgccggggcg
      361 agaaagaccg agaccgagcc gtcggtgtcg tcgtagcgca tcacgcgatc gttcggaatg
      421 tccgaccaga cgagatattt gcccgcggcg aaataggccg gcccctcggc ccagcggcag
      481 cctgtataga gcttttcgag atggacattg ccatgggcga gcttctcgaa acgctcgtca
      541 aggacctcga tgctcgacgt gaacatggaa cgcttcccct tacggacgcc gaccggcgct
      601 tgatcgccgg cacaatcgcc gaagcgccgc gcgcgcgcaa cccgccgcgc ggcgtcggaa
      661 gcctatcgcg tcttttcgtt gcgcaggcgc tcgtattcga agcgcaccag ctccgcgcca
      721 tgggcgcgct cgagccggcg caccgtgaaa tggccgcgcg cgatggtttg gaaatgctgg
      781 acgaaggcga ggttgaccgc ggcgccgccg accgctccga ccacgggcag ggcctgggcg
      841 gcgagctttt cgctgaccac caccccgaac cgcgcggcga tctgcgagac gaggcggacc
      901 agaaccggcg cggtctcgtt ggcgaggccg tgatggagaa tgaaccgcgc gctgtccctg
      961 agactgatcg ccagggcgcc gcgcgcggcg aaatattcgc tttcgagcgc cgcctcgccg
     1021 ggcgcgtggc cgcccaaggc gaagacctcg atgcaggcga gcgcggcctc gggcgcgctt
     1081 aaatcctcgc cttcggcccg cgcgatctgg gcgatcgagc gcagcatgat cgtcgtcgag
     1141 accggcaatt ccgcggcgag ggcggcgagg ccgaaggcgc cgccggcggc gccggacgcg
     1201 gcggccaggg ccttgtggag accgacctgc ggcttggccg gcgcgttcgg atcgatcgtc
     1261 ttgatggcga ggcgcaaggc ggtccgcagc gccctctcgc tcgcccgagc gatgactttg
     1321 cgcgccggcg agggcaaagc gcggcccgcc gcctggactt ggcggccgat gagatcggcc
     1381 atgcgcgtcg cgaagctgtc gccctcgagc gcgcgaaccg ccgcgcgcag ctcgttgagg
     1441 tcggcttcgc tcaattcctc ggtcatgatt tcgagcgtca tcgcgcgtct ctttggccgc
     1501 ccgaacgggc tctgtgaaat atggcgacgc cgaaggcgca gcggaagagg ctgcggccgc
     1561 tcttgccccg gatcagaacc gcgcgaagcg gcgggcggtc ctccgcgcca gggcaaagcc
     1621 ttgtcgcttc aaagacatag accgaaatgc gcgccgagcc ggcggccgat tccctcgatg
     1681 aaatattccg gcgtccaatg gcggaggaaa accagggcgc cgaaggcgag gaccagcgcc
     1741 gcgggcgaca ggagcgccat ggcgcgccag ggcagggaaa tgggcctggc ggccgcgccg
     1801 cgcagcaggt ggaacagggc ggttgcggcg atcccgatcg acaggccgaa ggcgacctct
     1861 tgcggcgagt gcgcgtgctg ggcgacccgg ccgatcccga tcagcacgat cagcagggcc
     1921 gcagcgccgc cgataaggac ttgctgcagc tttggccggc cggtcgcgac gataagggcg
     1981 atgcagccat agaccgcggc gctgaagctt tcatggccgc tcgggctttc catgcgcgga
     2041 tgcaggacat gcaagccgca gaggctgaag aacagcttgg cgccgatcgt cgcggccagg
     2101 ccggcgagca aggcgatcgc gaaagccgcc gcgtcgcgcc gcgctccgat ggcgataaga
     2161 tagaagagcg tcgccagcgc gagcgggccg agaatgacga catggccgag attgccgaaa
     2221 agatgcatca gatgagcgaa acccttgcgc ttctccgtcg tccttaagac gaaacgcccc
     2281 gaagcgacag gcgttgtcgg cccgcttacc gcttgcatcg cgtcttttcg gttaagatgc
     2341 gggcgaagat ttcgggcgaa ggccttcgcg catgttcgat ccatggatcc gattggcgcg
     2401 cgccgccacg cctgacggcg acgaattgat cttgcggcgg cgcggcggcg aatttgaaat
     2461 ccgcttcaat ggctgggagc tcatggcgag ccggacctcg cgctccgaag aagcgctggc
     2521 gcggctcgct ttccaggaat tgcggcggcc gccgcgcgcc gttctgatcg gcggcttggg
     2581 aatgggctat acgctgcgcg ccgcgctcga ccttgcgccg ccggacgctc gcatcgtcgt
     2641 ttcggagctc gtcccggcca tcgtcgaatg ggcgcgcggc cccctggcgg cgcttgccgg
     2701 ccgtccactc gacgatccgc gcgtcgaaat ccgcgtcggc ggcgtcgccg attctctcgc
     2761 ggcgtcgcgc catgctttcg acgccgtcct gctcgatacg gacaacgggc cggaagccgt
     2821 gctgcgcgag gcgaacggct ttctctattc ccgcgaaggt ctcgaatcag cgatcggcgc
     2881 cgtcgccggc gacggcgcga tcgccttctg gtcggccgac cgctcggaga agttcgagga
     2941 cgtactaaac gccgcggatg tgcaatggcg gcggctggac gtcgaggctc gccgcggcgg
     3001 gcccgcgcac acgatctatc ttgcgcggcc gccggcgaag gcgcgcggat agggagacct
     3061 ttgtagccgt ggtcgccgac cgcggccgac gcgaagcgtc ccgtttcacc ttgaagggag
     3121 gctacggcat gcacacatct cggcgcggcg gggccacatc ggcccggcat cgtcaagcgg
     3181 ccatagcccg accgaccgtt gcgcgaaccg accaagctct gcgcagcggg ctatggccgc
     3241 tgcgccgctt cgcttcgccc cttcgggtga cgacgccggg ccgatgcggc tttttgcatc
     3301 caagagcttt gccgacgaaa ccgtcggaaa gcccgacggt tcgtcggcaa agctcttgga
     3361 agccaaatcg cctgagccgc gcggagccgt caaggctcgc gagccatcgc gccgcgccaa
     3421 ctgattttct cctcagcctg ctttggcgag cgacgcgcga agcgcgcttg gccttgacgg
     3481 cgagcacggc tcaggcgcgc cgcgcttcaa gatatgtgca tgccgtagtc gcgaagcagg
     3541 ggagaaggta caggctcagc tcgaaatctg ctccagcgac agattagtcg tgcgcgggcc
//
LOCUS       DATGJW010000016         3873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_416823, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000016 DATGJW010000000
VERSION     DATGJW010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_416823"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..718)
                     /locus_tag="VKU03_00455"
     CDS             complement(<1..718)
                     /locus_tag="VKU03_00455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbon-nitrogen hydrolase family protein"
                     /protein_id="HLJ69755.1"
                     /translation="MKVSLVQMNSISDKAANIAAAKALIARAVAEEGPDWILLPEQFD
                     WAGGSKADKLANAETLPGGPAYAMAQDLARQHKVFIHAGSIMEKIDGENRIHNTSVVF
                     NREGEEIARYRKIHLFDVTTPNGVAYNESATVKPGEPAAITYDCEGVTIGCSICYDLR
                     FPDLFQALAEKGAQIIALPAAFTLQTGKDHWEPLLRARAIETETYVCAAAQTGSFTVG
                     NEQRQTYGHSLVADPWGHVVA"
     gene            complement(804..1301)
                     /locus_tag="VKU03_00460"
     CDS             complement(804..1301)
                     /locus_tag="VKU03_00460"
                     /inference="COORDINATES: protein motif:HMM:NF017856.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family protein"
                     /protein_id="HLJ69756.1"
                     /translation="MSLSQRIGATWRPKREHLWQTLRIIGACALAYLGAVLIGLPEIY
                     WSLITAVVVTQPGLSDTLAAGRDRIVATLAGAILGFVVLEISALGAPKLPLFWAALAP
                     LALLTAIKPNLRLASATLIVIVIVPSYGGVMVRPLDRVIEILVGVVASIVASAALDPA
                     RLWRR"
     gene            complement(1298..2074)
                     /locus_tag="VKU03_00465"
     CDS             complement(1298..2074)
                     /locus_tag="VKU03_00465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pseudouridine synthase"
                     /protein_id="HLJ69757.1"
                     /translation="MTPEETRARLLYRDGLMLVLDKPAGIAVHRGPKGGASLEDDFAA
                     LRFGLPRNPALAHRLDRETSGCLVLGRHRKALEKLGLLFKQGKIAKTYWAVVEGGPDA
                     PEGLIDLPLGRLDKSRGWWMKVDPAGAPAETRWRLLGRGEWRGRRIVWLALSPLTGRT
                     HQLRVHCAAKGWPILGDAVYGSAARSGGAPLHLHARRIEIPIVKAKPPVAVEAAAPVH
                     MREALEACGFSAADEKRPASPSGHAAQEAEAATLSLDPQR"
     gene            2262..3323
                     /gene="ftsY"
                     /locus_tag="VKU03_00470"
     CDS             2262..3323
                     /gene="ftsY"
                     /locus_tag="VKU03_00470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HLJ69758.1"
                     /translation="MSSDRDGEAKAGFFRRVFGGGRSEPAPPEASETSPAEAEPAETE
                     PASKRSWWSRLTGGLSRSSAAIGQGVTNIFTKRKLDAEALDDLEDILIQADLGVAAAS
                     RVREAVGSGRYDKAIEPSEVKAILAGEIEKILTPVARPLAIDHSKKPFVILVVGVNGS
                     GKTTTIGKLAAKFAAEGQKVTLAAGDTFRAAAIEQLRIWAGRTGAEIVAGAQGGDSAS
                     LAFEALSKARKDGADVLLIDTAGRLQNRAELMAELEKLIRVVRKIDPEAPHAALLVLD
                     ATVGQNALSQVETFGRVAGVTGLVMTKLDGTARGGILVALAEKFALPVHFIGVGESVD
                     DLEPFEARDFAEAIVGIER"
     gene            3397..>3873
                     /locus_tag="VKU03_00475"
     CDS             3397..>3873
                     /locus_tag="VKU03_00475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacylglutathione hydrolase family protein"
                     /protein_id="HLJ69759.1"
                     /translation="MTTHVHQFICLKDNFGVLIHDSASGATASIDVPDADAVIAAAAS
                     QGWEVTDLLITHHHADHVQGMPGLKSRYPKLRVAGPAKEAARIGGIDIQLNEGDLARV
                     GGLAAKVIETPGHTAGHISYWFEEDEIAFCGDTLFALGCGRVFETPMAVMWSSLMKL"
BASE COUNT          671 a         1246 c         1338 g          618 t
ORIGIN      
        1 tggcgacgac atggccccaa gggtcggcga cgagcgaatg gccgtaggtc tggcgctgct
       61 cgttgccgac ggtgaagctg ccggtctgcg cggcggcgca gacataagtc tccgtctcga
      121 tcgcgcgggc gcgcagcagc ggctcccaat gatccttgcc ggtctgcaag gtgaaggccg
      181 ccggcagcgc gatgatctgc gcgcctttct cggcgagcgc ctggaagagg tcgggaaagc
      241 gcagatcgta gcagatcgag cagccgatcg tgacgccttc gcaatcatag gtgatggcgg
      301 ccggctcgcc cggcttgacg gtggcgctct cgttataggc gacgccgttc ggcgtcgtca
      361 cgtcgaagag atggattttg cggtagcggg cgatctcctc gccctcgcga ttgaagacga
      421 cgctcgtatt gtggatgcgg ttctcgccgt cgatcttctc catgatcgag ccagcatgaa
      481 tgaagacctt atgctgtcgc gcgaggtcct gcgccatggc gtaagcgggg ccgcccggca
      541 gggtctcggc attggccagc ttgtccgcct tgctcccgcc tgcccaatcg aattgctcgg
      601 gcagcaaaat ccaatccggc ccctcctcgg cgacggcgcg cgcgatcagc gccttggcgg
      661 cggcgatatt ggccgccttg tcgctgatcg aattcatctg gacgagcgag actttcatcg
      721 aaggccctct gacgctgaag tcaatgtcgg agcgcggccg ctcggcgtca agcgggcgtt
      781 cggcgcccgg cggcggtcct cgatcagcgc cgccaaaggc gggcagggtc gagcgcggcc
      841 gaggcgacga tcgaagcgac aacgccgaca agaatctcga tgacgcgatc caacggacgg
      901 accatcaccc cgccatatga aggtacgata acgatgacga tcagggtcgc cgaggcgagt
      961 cgaagatttg gcttgatcgc tgtcaagagc gcgagcggcg cgagggccgc ccagaacaat
     1021 ggcagcttcg gggcgccgag cgccgagatt tcgagtacga caaagccgag gatcgcgccg
     1081 gcaagcgtcg cgacgatgcg gtcccggccg gcggcgagcg tgtcgctgag gcccggctgg
     1141 gtcaccacga ccgcggtgat gagcgaccag tagatttccg gcagaccgat cagcacggcg
     1201 ccgagataag caagggcgca ggcgccgatg atccgcagcg tctgccacag atgctcgcgc
     1261 ttcggccgcc atgtcgcccc tattcgctga gaaaggctca tcgttgcggg tccagcgaga
     1321 gcgtcgccgc ctccgcctct tgcgcagcat ggccggacgg cgaagccggg cgtttttcgt
     1381 ccgcggcgga aaagccgcag gcctccagcg cttcgcgcat atggaccggc gccgccgctt
     1441 cgacggcgac cggcggcttg gctttgacga ttgggatttc gatgcggcgg gcgtgaagat
     1501 gcagcggcgc cccgccgctc cgcgcggcgc ttccgtaaac cgcatcgccg aggatcggcc
     1561 agcccttggc ggcgcaatgg acgcgcaatt ggtgcgtacg gccggtgagc ggcgataaag
     1621 ccaaccagac gatgcgccgc ccgcgccatt cgcctcggcc gaggagccgc cagcgcgtct
     1681 cagccggcgc gccggccggg tcgactttca tccaccagcc gcggctcttg tcgagccggc
     1741 cgagcggcag gtcgatcagg ccctcgggcg cgtcggggcc gccctcgacc accgcccaat
     1801 aggtcttcgc aatcttgccc tgtttgaaga gcaagccgag cttttcgagc gccttgcggt
     1861 gccgaccgag aacgagacaa cccgacgtct cgcgatcgag ccggtgagcg agcgccgggt
     1921 tgcgcggcag gccgaagcgc aaagcggcga aatcgtcctc caggctcgcg ccgcccttgg
     1981 ggccgcgatg gaccgcgatt ccggcaggct tatcaaggac gagcatcagg ccgtcgcgat
     2041 agagaagccg cgctcttgtt tcttcgggcg tcatgaccca aaagctaccc gccgcgtcat
     2101 tcccgcgaaa gcggaaatcc agacggcgtt ggcgctgaaa cgcggcggtt ctccggattc
     2161 ccgcttttgg cgggaatgac atggagcttg atttaaggcg cggccgcccg cccgcggcgg
     2221 cgtcaaatgc gtgatcacga cagagcggaa ctgacgggtc gatgagttca gaccgagacg
     2281 gcgaggctaa ggccggcttt ttccggcgcg tctttggcgg cggccggagc gagccggcgc
     2341 cgcctgaagc cagcgagact tcgccggctg aggccgagcc ggccgagacc gagccggcgt
     2401 cgaaacgcag ttggtggagc cggctgaccg gcggcttgtc gcgctcctcc gcggcgatcg
     2461 gccaaggcgt cacgaacatt ttcaccaagc gaaagctcga cgcggaagcg ctggacgatc
     2521 tcgaagacat tctcatccag gccgatctcg gcgtcgccgc agcgtcgcgc gtgcgcgagg
     2581 cggtcggcag cggccgctac gataaggcga tcgagccgag cgaggtgaag gcgatcctcg
     2641 ccggcgagat cgagaagatt ttgacgccgg tggcgcggcc gctggcgatc gaccattcga
     2701 aaaagccctt cgtcattctc gtcgtcggcg tcaatggatc gggaaaaacc acgacgatcg
     2761 gcaagctcgc ggcgaaattc gccgccgaag gacaaaaagt gacgctcgcc gcaggcgaca
     2821 cgttccgcgc cgcggcgatc gagcaattgc gcatctgggc gggacggacc ggggccgaga
     2881 tcgtcgccgg cgcgcaaggc ggcgattccg caagcctcgc cttcgaggcg ctgagcaagg
     2941 cgcggaaaga cggcgccgac gtgctgctga tcgacacggc cggccgcctg cagaatcgcg
     3001 ccgagctgat ggccgagctt gaaaagctga tccgcgtggt caggaagatc gatccggaag
     3061 cgccgcatgc cgccttgctc gtgctcgacg cgaccgtcgg ccagaacgcg ctgagccagg
     3121 tcgagacgtt cggccgggtg gcgggcgtga ccggcctcgt catgaccaag ctcgacggca
     3181 ccgcgcgcgg cggcatcctc gtcgccctgg ccgagaaatt cgcgctgccg gtgcatttca
     3241 tcggcgtcgg cgagagcgtc gacgatctgg agccgttcga ggcgcgcgat ttcgccgagg
     3301 cgatcgtggg gatcgagcgg tgattccgta cccttcgtca gtcccttctc cccgcttcgc
     3361 ggggtgcgag ggggagccca tcacaaggag ccggtcatga cgactcacgt tcatcaattc
     3421 atctgcctca aggacaattt tggcgtgctc atccacgatt ccgcctcagg cgcgacggcg
     3481 tcgatcgacg ttcccgacgc ggacgcggtg atcgccgccg cggcgtcgca gggctgggag
     3541 gtgaccgacc tgctgatcac gcatcatcac gccgatcacg tccagggcat gcctggcctc
     3601 aaaagccgct atccgaagct gcgcgtcgcc gggccggcca aggaggcggc gcggatcggc
     3661 gggatcgaca ttcagcttaa cgagggcgac ctcgcccggg tcggcggcct cgccgccaag
     3721 gtgatcgaaa cgcccggcca tacggccggc catatttcgt actggttcga agaggacgag
     3781 atcgcctttt gcggcgacac gctgttcgcg ctgggctgcg gccgcgtctt cgagacgccg
     3841 atggcggtga tgtggagttc gctgatgaag ctc
//
LOCUS       DATGJW010000017         8534 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_417002, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000017 DATGJW010000000
VERSION     DATGJW010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8534)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8534)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8534
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417002"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(79..1410)
                     /locus_tag="VKU03_00480"
     CDS             complement(79..1410)
                     /locus_tag="VKU03_00480"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HLJ69760.1"
                     /translation="MRLWIKDPLAIFAEGAERGLVVEDARIVELVGAGKTPVAPVAAT
                     FDASRHVVLPGLVNAHHHFYQTLTRAHPAGLNQELIPWLVALNTIWARLKPEHFRVAV
                     RMALIELMLSGCTTAADHHQMFPRGLENAIDIEIEEARKLGVRMTVTRGSQNVSEKDG
                     GLPLDEIVQDEDSILADSERVLKLFHDPRRGALIRIALAPCSPLAVTKGVMIGSAALA
                     ERYDCQIHTHLAQIPDEEAYCRQAFGMGLVDKLEEIGWLGPRLWLAHGSCFEAQDVAR
                     LGAAGVGVCHCAASDMLLGLKICPTKDLEAAGATIGLGVDGSASNDSSNAMEALRHSL
                     MLGRLRYGASGVTHRDAIRWATEGSARCLGRSDIGRIAAGFEADLAMFTLDEPRFSGA
                     HDPLAALVLCGAHRADRVMIAGQWRVIDGAPVGLDLEALLAEHRAAARAFA"
     gene            1555..2667
                     /locus_tag="VKU03_00485"
     CDS             1555..2667
                     /locus_tag="VKU03_00485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ69761.1"
                     /translation="MTSENGLGEEILIARSAVREEIMGDDKPTDGGSASGIWRAAAGL
                     ALCAGVIASPSAAWALDKVTFGTNWLADPEAGGFYQAVVDGTYAKYGLDVTIMPGGPQ
                     TNGGLLLVAGKIEFFMTGDMLGDFLEVEQGIPTIPVAAEFQKNPEIMMSHPSVGLDKW
                     TDLPKATAYYVSQGAVTSFFAWMRSAWGFKEENVKPYTFNSAPFIADKHSIQQGYVTS
                     EPFEVEKQGGFKPNIFLLADYGYSSYSTLIEARRDLVQKNPDLVQRFVDASAIGWYHY
                     LYGDNKKANEAIKKDNPEMTDEQIAYSIAKLKEYGIVDSGDTLKLGIGAMNEARIKDF
                     FDKMVKAGIVKPDTDYKKAFTLQFVNKGVGVDLRPK"
     gene            2767..3396
                     /gene="folE"
                     /locus_tag="VKU03_00490"
     CDS             2767..3396
                     /gene="folE"
                     /locus_tag="VKU03_00490"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HLJ69762.1"
                     /translation="MANTTKLAAQPAKRSEDFRPTDAAAEEAFRSIIRWVGEDPDRDG
                     LHETPQRLVRAFREYFCGYDEDPEEILKKTFSEVEGYDEMVVLRGVPFESHCEHHVAP
                     IIGRAWVGYVPSRRVVGISKLARVVEAYARRLQIQERMTAQIANAIETVLKPVGVAVV
                     IKATHHCMLSRGVKKHGADLVTSRMLGVFRDNQMTRSEFLALVNSDVEV"
     gene            complement(3494..3970)
                     /locus_tag="VKU03_00495"
     CDS             complement(3494..3970)
                     /locus_tag="VKU03_00495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69763.1"
                     /translation="MEFATKEEAEAFLAKALPKATAGNPKYRAADGGLTQWLTRQVVF
                     GPGASPSGVAVEMKEDVLQFRDGRQTSKGTHEVRFFIEDVQISELADSADVTENGEKA
                     LGIIFRCNSGKCIEAKWDGVPSPSDWSDISIQDPAMRTKILGAFQALKRVAGNRAE"
     gene            4390..6057
                     /locus_tag="VKU03_00500"
     CDS             4390..6057
                     /locus_tag="VKU03_00500"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ69764.1"
                     /translation="MSANLDTAAEADVSRWGAFGQPAFRVIWLTSIVANIGLAMFDTG
                     SGWLMTSLNANPMAVSMVQVVTTLPMFLFTLPAGALADIIDPRRLLILVEIVVTIVAA
                     IFASAVSLQLATPAALLLTAFLLGAGGALTAPAWSAITPLLVPREELDNATAANSVGY
                     NLSRAVGPALGGIVIATSGIASPFWAFTASNLVIIAGLLWWRPKPKCSQSLPAERLTS
                     AVRTGLRYAANNPHLRATLMRAVAFFPFASAYWALLPLVARHQMHNGPEVYGLLLGAI
                     GLGAIIGSLTLGWLKAAFGPDGLGAVGTLATAIALVLFGMAHHSALAFCAAVIAGASW
                     TIVLATLYVSAQIALPDWVRGRGLAVFLTVYFGAMTAGSAIWGHIAGMESLSTALFIA
                     AAGAVVAVPLTWRWKLQTAAGLDLTPSMHWRAPAFKLKVENDQGPVLVTVEYRTDTKD
                     RAAFLAAIEEMGYERKRDGAFAWGVFEDPVDPDRFVETFLIESWLELRHLRERVTNAD
                     RLLEEEVRKLLLAPPKVGFLVASDRPRRRRKSRAPQQIAPPMATAAE"
     gene            6124..7083
                     /locus_tag="VKU03_00505"
     CDS             6124..7083
                     /locus_tag="VKU03_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ69765.1"
                     /translation="MKTALCLTPDRAFFRPAICTAATILAQDDADRFDVFVVCESEDV
                     ATGVDRLAPDLRRRIKILCADFSAFDRGLRAEGRFSRAVFRRLFLDRVLPADYERIVS
                     IDSDVWVARPGLGRLAALDFGGAPFAAAYDMIFLMDFARNTLAKQFQADRAALGLAPT
                     TPYFNAGVIAIDRRKWSAMALGERVVAALRRAPERYPFLEQSALNEIIAGGFAPLSPR
                     YNFMGDFFLLDLEEAFQPIALHFVNAPKPWQYELWRGERRFAENYRAWFAASPWPELA
                     EDGGRLAAAPARKPRLTPQRRAFAERLTAYLRTRAFIDGWRAP"
     gene            7099..7518
                     /locus_tag="VKU03_00510"
     CDS             7099..7518
                     /locus_tag="VKU03_00510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="secondary thiamine-phosphate synthase enzyme
                     YjbQ"
                     /protein_id="HLJ69766.1"
                     /translation="MRQAADILPIQTSGRGLREITPDIAAWVDRQNVSTGLLTLFCRH
                     TSASLLIQENAAPAVKTDLENFFAAIAPEDPSRYAHNDEGPDDMPAHLRAALTGVNLA
                     VPIIGGRLQLGTWQGVYLFEHRRRPHRREIALHVIGE"
     gene            7640..>8534
                     /locus_tag="VKU03_00515"
     CDS             7640..>8534
                     /locus_tag="VKU03_00515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016799 - hydrolase activity, hydrolyzing
                     N-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HLJ69767.1"
                     /translation="MDVDTGTDDAVAIMLAALHPELELLAVTTVNGNVEVAHCTDNTL
                     RTLEWIGRADIPVYEGLAHPIVRSDFPTPRAAKRDAGVHMEALPLPEAKGLKQKLPAP
                     LFLTEVFAQNPREITLVAVGPLSNLAAAIALDPTFVGNVAELMIMGGAINKSNVTPSA
                     EFNIWADPEASAIVLSAGWRKATMVPLDATHEALISLDQCRRLSALGSPAGEAAAKFI
                     EYRIAGYQANQPTGIPDTAPVHDAVCIAALVDDKVISTRHLNVVVETRGEHTVGRTVV
                     DHEHRTKRPANCHVALHADRER"
BASE COUNT         1432 a         2790 c         2753 g         1559 t
ORIGIN      
        1 tatagtcgcc caatccattc cccgagcgcc gcgctccaaa tagatggccc cgcggcgctc
       61 gcaaacatgg cgtatctctc aggcaaacgc ccgcgccgcg gcgcggtgtt cggcgagcag
      121 cgcctccaaa tcgagcccga ccggcgcgcc gtcgatcacc cgccattggc cggcgatcat
      181 cacccggtcg gcgcggtggg cgccgcagag gaccagggcg gcgagcgggt cgtgcgcgcc
      241 ggaaaagcgc ggctcgtcca gcgtgaacat cgccaggtcc gcttcgaacc cggcggcgat
      301 gcgcccgatg tcgctccggc cgaggcagcg cgccgaacct tccgtcgccc agcggatcgc
      361 gtcgcgatgg gtgacgccgg aggcgccgta gcgcagccgg ccgagcatca ggctgtggcg
      421 gagcgcctcc atcgcgtttg aggaatcgtt ggaggccgag ccgtcgacgc cgagtccgat
      481 cgtcgcgccg gcggcttcca ggtccttggt cggacagatt ttcaacccga gcagcatgtc
      541 ggaggcggcg caatggcaga cgccgacccc tgccgcgccg agccgcgcga cgtcctgcgc
      601 ctcgaagcag gagccatgcg cgagccagag gcgcggcccg agccagccga tctcctccag
      661 cttgtcgacg agccccatgc cgaaggcctg gcggcaatag gcctcctcgt cggggatttg
      721 cgcgagatgc gtatggattt ggcaatcgta gcgctcggcg agcgccgccg agccgatcat
      781 cacgcctttg gtcaccgcca gcggcgaaca aggcgcgagc gcgatgcgga tgagggcccc
      841 gcgcctggga tcgtgaaaca gcttcaagac gcgctcgctg tcggcgagga tcgagtcttc
      901 gtcctggacg atctcgtcga gcggcagccc gccatctttt tccgacacgt tctgggagcc
      961 gcgcgtcacc gtcatccgta cgccgagctt gcgcgcttcc tcgatttcga tgtcgatcgc
     1021 gttttcgagg cctcgcggga acatctgatg atgatcggcc gccgtcgtgc agcctgacag
     1081 catgagctcg atcagagcca tgcggaccgc gacgcggaaa tgctcgggct tcagccgcgc
     1141 ccagatcgtg ttgagagcga caagccaggg aatgagctcc tggttgagcc cggccggatg
     1201 ggcgcgggtc agggtctggt agaagtgatg atgcgcgttg acgaggccgg gcaggacgac
     1261 atggcgcgaa gcgtcgaatg tcgccgcgac gggggcgacc ggcgtcttgc cggcgccgac
     1321 gagctcgacg atccgcgcat cctcgacgac gaggccgcgc tcggcgcctt ccgcgaaaat
     1381 cgccagcgga tctttgatcc agagacgcat ttgaaccctt aaggcgccgg acaaggcgcg
     1441 gcttgatcct tcgcccaagg cgcggcgtct gtccatgccg gaagcggcct ccttattgct
     1501 tggcgccggc cgcgagcgct cgcctcttgc ggggagctgg ggactaagtg caacatgacg
     1561 agcgaaaatg ggctcggcga agagattctc atcgcgcgaa gcgccgtcag ggaggaaatc
     1621 atgggtgacg acaagcccac ggacggcggt tctgcgtccg gaatttggcg agcggccgcg
     1681 ggcttggcgc tctgcgccgg cgtgatcgct tcgccgagcg ccgcctgggc gctcgacaag
     1741 gtgacgttcg gcaccaattg gctcgccgat cccgaggcgg gcggctttta tcaagcggtc
     1801 gtcgacggca cctacgccaa atacggcctc gacgtgacca tcatgccggg cggtccgcag
     1861 accaatggcg gcttgcttct ggttgccggt aaaatcgaat tcttcatgac tggagatatg
     1921 cttggcgact tcttggaagt cgaacagggc atcccaacga tcccggtcgc cgccgagttc
     1981 caaaagaacc cggaaatcat gatgtcgcat ccgagcgtcg ggctcgacaa atggaccgat
     2041 ctgcccaaag cgaccgctta ctacgtgagc caaggcgcag tgacctcatt ctttgcatgg
     2101 atgcgctccg cctggggctt caaggaggaa aacgtcaagc cctatacgtt caactcggcg
     2161 ccgttcatcg ccgacaagca ttcgatccag cagggttacg tcacctcgga gccgttcgag
     2221 gtggagaagc agggcgggtt caagcccaat atcttccttc tcgccgatta cggatattcg
     2281 agctactcga cgctgattga ggcccgccgc gacctcgtcc agaaaaatcc cgatctggtt
     2341 cagcgcttcg tcgacgcctc cgccatcggc tggtaccatt atctttacgg cgacaacaaa
     2401 aaggccaatg aggcgatcaa gaaggacaat cccgaaatga ccgatgagca gatcgcctat
     2461 tcgatcgcca agctcaagga atacggcatt gtcgattccg gcgacacgct gaagctcggc
     2521 atcggcgcga tgaacgaggc gcggatcaag gatttcttcg acaagatggt gaaggccggc
     2581 atcgtcaagc cggacacgga ttataagaag gccttcacgt tgcaattcgt gaacaagggc
     2641 gtcggcgtcg atcttaggcc gaaataaggc ggcgcgccgg cggcgcatat ttcgctcgca
     2701 tcgcagccgc aaggcgcgtt aaaagcctcg ccgaagcgcg gcccaacgcg cttagaagga
     2761 tgtcccatgg cgaatacaac caagctcgcc gcacaaccgg cgaagcggag cgaggatttc
     2821 cggccgaccg acgcggcggc cgaggaggcg tttcggtcga tcatccgctg ggtcggggaa
     2881 gatccggacc gcgacggctt gcatgagacg ccgcagcgcc tcgtgcgcgc ctttcgcgaa
     2941 tatttctgcg gctacgatga agacccagag gaaatcctga aaaagacctt ctcggaggtc
     3001 gagggctacg acgagatggt cgtgctgcgc ggcgtgccct tcgagagcca ttgcgagcat
     3061 catgtcgcgc cgatcatcgg ccgcgcctgg gtcggctatg tgccgagccg ccgcgtcgtc
     3121 ggcatatcca agctggcgcg cgtcgtcgaa gcctatgcgc gccgcctgca gattcaggag
     3181 cggatgacgg cgcaaatcgc caacgcgatc gagacggtgc tgaagcctgt cggcgtcgct
     3241 gtggtgatca aggcgacaca tcactgcatg ctgagccgcg gcgtcaaaaa acacggcgcc
     3301 gatctcgtca ccagccggat gctcggcgtt tttcgcgaca atcagatgac ccgcagcgag
     3361 tttctcgcgc tggtcaattc cgacgtggaa gtctgaccac gccagtcatt tccgcgcatc
     3421 cgggatccag agaaccgccg cgcctgcagg atagattccg gctttcgctg gaatgatata
     3481 acggccgcga acgctattcc gcgcggtttc cggcgacgcg tttcagcgcc tggaaggcgc
     3541 cgaggatttt cgtccgcatc gctgggtcct ggatggaaat gtccgaccag tcgcttggcg
     3601 agggaacgcc gtcccatttc gcttcgatgc attttccgga attgcagcgg aagatgattc
     3661 caagcgcctt ctcgccgttt tccgttacat cggcgctgtc ggcgagctcg gaaatctgca
     3721 cgtcctcgat gaaaaagcgg acctcgtgcg tacccttgct ggtctgccgg ccgtcgcgaa
     3781 attgcagaac gtcctctttc atttcgacgg cgacgccgct cggcgacgcg cccggcccga
     3841 aaacgacctg ccgcgtcagc cattgggtga ggccgccatc cgcggcgcga tatttgggat
     3901 tgcccgccgt ggctttgggc agggccttgg ccaggaacgc ctcggcctcc tcctttgtgg
     3961 caaattccac attttgaaca cgagattgag cgttaagcat tgcggcctcg gaggcggagc
     4021 gcggagcggc ttcggcggcc ggcgcgaggg cggtcgcgct aaggagcagg cggccaccgg
     4081 cagccgcgag aacgaggact ctgggacgcg ccatgcttcg attgtccaat tcgtgagcgg
     4141 atgcgtcttg agcccgccgc cttcgaccac tatctgcggg ccacgcatgt tgatgatggg
     4201 gcagtttggc ggatgagtcg attcttggcc aaggccagcg gaccggcgca gggctcgacg
     4261 agcctccaaa cggccgaatg gttcgccgtc cggcgaacaa gtcgccggcg ttccgatttg
     4321 agtgattgat ttccttagac ctgcttgcgg gaatcggttt ggcgcggcgc gccgcgccgg
     4381 gagagcgatt tgagcgcgaa tttggatacg gcggcggagg cggatgtctc gcgctggggc
     4441 gcattcggcc agccggcttt ccgggtgatc tggctgacga gcatcgtcgc caatatcggc
     4501 cttgcgatgt tcgacaccgg ttcgggctgg ctgatgacca gcctcaacgc caatccgatg
     4561 gccgtctcga tggtccaggt ggtcaccacc ttgccgatgt tcctgttcac gctgccggcc
     4621 ggcgcgctcg cggatattat cgatccgcgg cggctgctga ttctcgtcga gatcgtcgtc
     4681 accatcgtcg ccgcgatttt cgcctccgcc gtgtcgctgc aattggcgac ccctgccgcg
     4741 cttctgctga cggcctttct gctcggcgcc ggcggcgcgc tgaccgcccc cgcctggtcg
     4801 gcgatcacgc cgctgctggt gccgcgcgag gagctcgaca acgccaccgc cgccaacagc
     4861 gtcggctaca atctgtcgcg cgccgtgggc ccggcgcttg gcggcatcgt catcgccacg
     4921 tcgggaatcg cctcgccgtt ctgggccttc acagccagca acctcgtcat catcgccggc
     4981 ctcctgtggt ggcggcccaa gccgaaatgt tcgcagagcc tgccggccga gcgcctgacc
     5041 agcgcggtgc gcaccggcct gcgctacgcc gcgaacaacc ctcatttgcg cgccaccttg
     5101 atgcgcgcgg tcgccttctt tcccttcgcc agcgcctatt gggcgctgct gccgctggtc
     5161 gcgcgccatc aaatgcacaa tgggccggaa gtttacggcc tgctgctcgg cgcgatcggc
     5221 ctcggcgcga tcatcggctc gttgacgctc ggctggctca aggctgcttt cggccccgac
     5281 gggctcggcg ccgtcggcac gctcgccacc gccatcgctc tcgtcctgtt cggcatggcg
     5341 catcattccg ctctggcttt ctgcgccgcg gtcatcgccg gcgcctcctg gacgatcgtc
     5401 ctcgccacgc tctatgtctc ggcccagatc gccctgccgg actgggtgcg cgggcgcggc
     5461 ttggcggtat tcttgaccgt ctatttcggc gcaatgaccg ccggcagcgc gatctggggc
     5521 catattgccg ggatggagtc gctttcgacc gccctgttca tcgccgcggc cggcgccgtc
     5581 gtcgcggttc ccttgacctg gcgctggaag ctgcagaccg cggccgggct cgacctgacc
     5641 ccgtcgatgc attggcgcgc gcccgccttc aagctgaaag tcgagaacga tcagggcccg
     5701 gtcctggtga cggtcgagta tcggaccgac accaaggacc gcgccgcctt tctcgcggcg
     5761 atcgaggaaa tgggctacga gcgcaagcgc gacggcgcct tcgcctgggg cgtgttcgag
     5821 gatccggtcg atccggatcg cttcgttgag acctttctga tcgaatcctg gctcgagctt
     5881 cgccatttgc gcgagcgggt gacgaatgcc gaccgcttgc tcgaagaaga agtccgcaag
     5941 ctgctgctcg cgccgcccaa ggtcgggttc ctggtcgcca gcgatcggcc gcgccggagg
     6001 cgcaagagtc gcgcgccgca acaaatcgcg cctcccatgg cgaccgcggc ggaatagaag
     6061 cggctttttc ccgcgcatgg cgcgtgctac agcctcgcgc cgctcttccg tttcccagcc
     6121 ttcatgaaaa ccgctctctg cctgacgccg gaccgcgcct tctttcgccc ggcgatctgc
     6181 acggcggcga cgatcttggc gcaggacgat gcggaccgct tcgatgtttt cgtcgtttgt
     6241 gagagcgagg acgtagcgac gggcgtcgac cggctcgccc ccgacttgcg ccgccgcatc
     6301 aaaattctct gcgctgattt ttcggctttc gaccggggct tgcgcgctga ggggcgcttc
     6361 tcgcgcgccg tgttccgccg cctgtttctc gatcgcgtgc tgccggccga ctatgagcgc
     6421 atcgtctcga tcgattcgga tgtttgggtc gcccggcccg ggctcggccg attggcggcg
     6481 ctcgatttcg gcggcgctcc gttcgccgcc gcctatgaca tgatcttcct gatggatttc
     6541 gcgcgcaaca cgctcgccaa gcaatttcag gccgaccgcg ccgcgctcgg ccttgcgccg
     6601 accacgcctt atttcaatgc cggggtgatc gcgatcgatc gccgaaaatg gagcgcgatg
     6661 gcgctcggcg agcgcgtcgt cgccgccttg cgccgtgctc cggagcgtta tccgttcctc
     6721 gaacagagcg cgttgaatga aatcatcgcc ggcggcttcg cgccgctgtc gccgcgctac
     6781 aatttcatgg gcgatttttt cctgctcgat ttggaggagg cgtttcagcc gatcgcgctg
     6841 catttcgtca atgcgcccaa gccctggcaa tacgaacttt ggcgcggcga aaggcgtttt
     6901 gccgaaaact accgcgcctg gttcgcagct tcgccttggc ccgaattggc cgaggatggc
     6961 ggacggctcg ccgccgcgcc ggcgcgcaag cctcggctga cgcctcagcg gcgcgccttc
     7021 gccgagcggc tgaccgccta tctgaggacg cgcgccttca tcgacggatg gcgcgcgccg
     7081 tgaattggga gtttggccat gcgtcaagcc gccgatattc tgcccattca gacgagcggc
     7141 cggggtctgc gcgagatcac gcccgacatc gccgcctggg tggatcggca gaacgtctcg
     7201 accggcctgc tcactttgtt ctgccgccat acgtcggctt cgctgctgat ccaggaaaac
     7261 gccgcgccgg ccgtcaagac cgatctggag aatttcttcg cggcgatcgc gccggaggat
     7321 ccgagccgtt atgcgcataa cgacgaaggg ccggacgaca tgccggcgca tttgcgcgcg
     7381 gcgctgaccg gagtcaatct ggccgtgccg atcatcggcg ggcgattgca gctcggaacg
     7441 tggcagggcg tctatctctt cgagcaccgc cgccggccgc atcggcgcga gatcgcgctg
     7501 catgtgatcg gagagtagcg ctcatgtcat tcccgaggag gcgtttcggc tctccggatt
     7561 cacgcttaac ccgggaatga caggcgcgtg ggcgtgaggg tacaaaggga gacgggagcg
     7621 catggcgagg aagcttgtct tggacgtcga caccggcacc gacgacgcgg tggcgatcat
     7681 gctcgccgcg ctgcatccgg agctggagct cctcgccgtg accaccgtga acggcaatgt
     7741 cgaagtggcc cattgcacgg acaacacgct tcgcacgctc gaatggatcg gcagggcgga
     7801 cattccggtc tatgaaggcc tcgcccatcc gatcgtgcgc agcgatttcc ccacaccgcg
     7861 cgcggcgaag cgcgacgccg gggtccatat ggaggctctg ccgctgccgg aggcgaaggg
     7921 actcaagcaa aagcttccgg cgccgctctt cctgacggaa gtcttcgcgc aaaatccccg
     7981 cgaaataacc ctggtcgcgg tcgggccctt gtcgaatctc gcggcggcga tcgcgctcga
     8041 tccgactttc gtcggcaatg tcgccgaatt gatgatcatg ggcggagcga tcaacaaatc
     8101 caatgtcacc ccctccgccg agttcaacat ctgggccgat cccgaggcgt ccgccatcgt
     8161 cctctccgcc ggctggcgaa aggcgacgat ggtcccgctc gacgccacgc atgaggcgct
     8221 gatctcgctc gaccagtgcc ggcgcctcag cgcgctcggt tcgcccgccg gcgaggcggc
     8281 ggccaagttc atcgaatatc ggatcgccgg ctatcaggcc aatcagccga ccggaattcc
     8341 cgacacggcg ccggttcacg acgccgtctg catcgcggcg ctggtcgacg acaaggtcat
     8401 ctcgacccgt catctcaacg tcgtcgtcga aacgcgcggc gaacatacgg tcggccgcac
     8461 cgtggtcgat cacgagcatc gcacgaagcg gcccgccaat tgccacgtcg ctttgcatgc
     8521 ggatcgggag aggt
//
LOCUS       DATGJW010000018        25271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_728335, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000018 DATGJW010000000
VERSION     DATGJW010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 25271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 25271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..25271
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_728335"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..723
                     /locus_tag="VKU03_00520"
     CDS             <1..723
                     /locus_tag="VKU03_00520"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ69768.1"
                     /translation="VIKQAADKGIVVVAVDQAVSAPEAYVLTNDQVAYAKVGAKWLFD
                     KLGGKGNVIEMRGIDGVPADADRHNGFEQVLKDYPNIHVTSVFTGWALNKTAQETKDL
                     LASGKEIDGIWTSGIDSTVVDAYQTAGKPFVPVVGADNNGFIGQLINLKDKGLTGAAV
                     SNPPAVGGAGLGLALDVLQKKPHPHVVKITPTVWDNTSAAGMEELKKAYNPKLDPFYS
                     VAINVPPYTHYTMQQILACKGP"
     gene            795..2294
                     /locus_tag="VKU03_00525"
     CDS             795..2294
                     /locus_tag="VKU03_00525"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ69769.1"
                     /translation="MSPDQDSILHISGVAKRFDSVVALRSAELKVRAGEVHALMGANG
                     AGKSTLVKIITGVFPADSGDIVIAGERKVFRSPAEARQAGIVSVYQDPAIVPDLTVAQ
                     NMRLARVGLDAVRQSMRSLGVERIDLSRAAREIDFATLRLIDLSRALASNPRILLLDE
                     ITAALPIDLAERVFAVVRDWRQRGASIIFISHRMAEVAALCDRATVLRDGVTVGVTAT
                     KGAEDKIVNMMLGAEVAKAAVSAGARAPKAALAAASALEVKDLACGHGLRGVSFAVRP
                     GEVLGVAALEGQGQEELFDCIAGVRRADAGEIWADGKPLRARHPADAVRAGLVLVPAN
                     RLQALLQQRSIRENVALPIFGRVKNWGPIAMKDERRRVDDAVRRLQIDTRASSELRRL
                     SGGNQQKVVIARWLASGFRTLLCFDPTRGIDVGTKRQIYELLREVARQGASVLLFTSE
                     LPEIRLACDRAIVLFGGRISGEMTADEADEARLLRAAHGLTAEAAEARA"
     gene            2291..3277
                     /locus_tag="VKU03_00530"
     CDS             2291..3277
                     /locus_tag="VKU03_00530"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ69770.1"
                     /translation="MSEASLRESGGLVRMLSRRSRLLGMPALLAAAILATAFIHPDFG
                     SFDIQSLALGALPLAMAAAAQAIVVISGGIDLSVGSLIAVSNVLAASLMQHASFGASL
                     GIAVAVLALGAAQGAINGLTVAISRIPDVVVTLTTGFIWGGVALLILERPGGGAPAAF
                     LNIAAGADVSAWIPNALIVMVVVIAAIWLPIKLSRIGLLLYASGSDPVAAFRSGVDTR
                     LARTLAYTFGGVACAAGGLSLTMTTGIGAPLAGTYYTLSSVAAAVIGGVSLNGGRGGM
                     VGPVIAALLLTLIPMDLVFLGIDPNFGQVIQGTLIVLVVMIGGFVTFVQSRK"
     gene            3274..4215
                     /locus_tag="VKU03_00535"
     CDS             3274..4215
                     /locus_tag="VKU03_00535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ69771.1"
                     /translation="MNAALQFLKTRTIVALILLLAIFLIGIEIVRPGTVTPLWGSNAL
                     LFAAPLAIMGAGQTLVMLTGGIDLSVASVATGAAYLLATNASSGDSFAIVIALALGLI
                     VGVVNGIGVAVLRVQPLVMTLGTGLMTGGVMIVYSQQMLASQPHVPAVIQTLGAGKVL
                     GLVPVDLFLWIVIAALMLYGLNRTGFGRLIYAVGDNREACHLAGIRVWRVLFVDYLLC
                     ALLAAIAGLVLVGGTNAADLSLADAYLLPSVACVIIGGTSIFGGRGGYSGTIVGALIL
                     TALTSLLTLLDVPEPVKQILYGAIILSLAAAYARLTE"
     gene            4409..6679
                     /locus_tag="VKU03_00540"
     CDS             4409..6679
                     /locus_tag="VKU03_00540"
                     /inference="COORDINATES: protein motif:HMM:NF017964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching N-terminal
                     domain-containing protein"
                     /protein_id="HLJ69772.1"
                     /translation="MKPSIVNRLSVDPDKIIIFRGGHVVIANWRGLIVSQDQGYYVHH
                     TRFLSRFDLRLDGRSVQPVCCNRIAPHAAMGYYLAPSPAGREAAPPGDDDPSGGEVVQ
                     KGIEIKVRTYAGGGCHQDIVLVNHGLAPATTSLDLVFAADFADLEGVTGGERRQDAPI
                     RRNFKELGHGRGELTFSYEHPKLNHATRIVVAGPGGLVDDGAALRITLALRPQAPETI
                     AVDIAPIFLGEQIQPWFGIGGEPTAHHPDQARLERWLDGCVEVAFANRAVQAAWNRAA
                     TDLWSLQGLRGEGEELFTPIAGIPKYTGLFGRDALVAGLQSAMLNPSTLRGTLSTLAR
                     WAAKTLDDRFDAEPGKILHQRQLGPLALLGKNPFLHYYGDYSAAGLFVIGAALHFAHC
                     GDRDAFEAFRNPVLAALEWMDRDGDKDGDGFYEYQTRAGDSGIKNQGWKDSNQAILYP
                     DGSFTPNPVAVAEIQGIYYAAKQSMACLFATIGEREAADKLFGQAAALKRRFNERFWM
                     EDLGFFALALGPDKKPVKTIASNVGSCLAYGIVDEDKAKAVADRLLAPDMFSGWGVRT
                     LSSEHPAFNPLSYHLGSIWPAANANICFGLKRYGFNDACHKIAKAIFDAAGLFEHDRL
                     PEVFGGHPRDKDHPHPGVYPESCWPQAWSASAVIQICHALAGLTFAAPLNALVLDPAL
                     PDWLPKMTIDNLIVGGKRAKIEVERDASGAVAHRVVEAADLRIVRLDPDLPPGKDRLA
                     HAAKKLAQSQMASNPV"
     gene            6875..7525
                     /locus_tag="VKU03_00545"
     CDS             6875..7525
                     /locus_tag="VKU03_00545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HLJ69773.1"
                     /translation="MAVFGPRANLAVNAALVGAVGLLLVLLLMIWVVPVTGYNTLERY
                     TPIQPVPFSHKHHVSGLGLDCRYCHTTVETSQNAGIPPTKTCMTCHSQIWTNAAILAP
                     VRQSLADGKPLRWTRVYGLPDYVYFDHSIHIAKGVGCTECHGPIGDMPLTWKATTLYM
                     SWCLSCHRDPAPHLRPLNEVFNPHWRRSPATPSGAELMATYHIHTETLSDCGTCHR"
     gene            7513..10449
                     /locus_tag="VKU03_00550"
     CDS             7513..10449
                     /locus_tag="VKU03_00550"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAT-variant-translocated molybdopterin
                     oxidoreductase"
                     /protein_id="HLJ69774.1"
                     /translation="MPSMSAAGKAYWRSLEDLAESPAFAAAIEREAPRLRDLVNSVDR
                     RRFLQLMAASIALSGLTGCGPEANLRQLLPYVEQPEGIVPGRSRYYATATTLEGYATG
                     VLIAHEMARPIKVEGNPDHPASLGAAGAIMQATILSLYDPQRAQSIVGNRQIDTWQGF
                     IAALHDRRAALGQTHGEGLRLLTGAVSSPSLVAQIADLQKQFAGMRWHQWEPLARDNQ
                     WTALQQAFGRPVETIFRLDRADVVFAVESDLISSTPGWLAYARQFAARRRPTETNGAM
                     SRVYAIESTPTLIGAKADHRLALRPAEIAAALRIVAGTVGAGPKDWIAIEAANADWFK
                     AAAEDLIQHKGRALVHVGREQPLEIHQLAAAINGALGAFGGTIGVIEPVIASPAPHIQ
                     SLRDLVDDMKAGRVDTLILIDTNPVYAAPADFEFADALAKVKFSASLSLYADETAHAC
                     LWRVPLAHEYEAWSDARAFDGTITIQQPQVRRLYGGHSAQEALAALQGNTQPDDHKLL
                     REFWRGKAQQENRGDFEQFWRDSLRAGLVADSAAAPVTAPLKSDLAGAAPASTPNASL
                     IELLFRPDESLWDGRFADNAWLLETPRAFTRLTWDNAALIAPQTAKRLGLADGDVVEI
                     ATQHGKLKAPAFVLPGQAPDCVTLPLGFGRRAGGLSVGQGFDAYSIRGAGAPWLAPAA
                     SIVKTGDTARLATVQGHDRVAGRDLIREGDLADFRQNPDFLQRKTSEESLYPGYDYPG
                     RAWAMAIDLNSCIGCQACVVACQAENNVPTVGKDLVIGQRIMHWLRIDRYYSGPADAP
                     SVAFEPMPCMHCENAPCEVVCPVHATTHDHEGLNLMVYNRCVGTRFCSNNCPYKVRRF
                     NFFAFADRTDRPPESWNPQVSVRGRGVMEKCTYCIQRIRNAEIDADRDNRPLRDGEVV
                     TACQQSCPTQAIVFGDRNDRGSAVAKRKAAPTDYTLLDELNTRPRTSYAALIRNPNPR
                     IEGEKT"
     gene            10446..11807
                     /gene="nrfD"
                     /locus_tag="VKU03_00555"
     CDS             10446..11807
                     /gene="nrfD"
                     /locus_tag="VKU03_00555"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HLJ69775.1"
                     /translation="MTLAADVPRPAPVVEPGVTLRSLSEQVSGRLLSRRPPLWWWVGF
                     GWALALLLVLIVSVTNLFVTGIGIWGVNIPVAWGLAIAEYVWWIALASGGTIVSALFH
                     VTRSPWRSATNRIAETMLLSAAACAGIMPILHLGRPGLFYWLFPYSTVMGVWPQVRSP
                     LWWDFVCLLCYILMSIMYYYVGLVPDVATVRDLAQSRSKKVFYGVLAMGWRGSARHWR
                     KHKTVYAIMSAIMAPMVISVHSVVGLDFAAGLTPGWHSTQFPPYFFFGAVISGTALVV
                     MLTILVRWGYGLENVITDYHLNALGKLVLVGSVMLGYAYLFEAWGPIYGADAADKTEF
                     ASRVFGFTAGAYWGKIALNILIPQLLWLPAIRRSKAALFLICAGIIVGMWLERYIIVV
                     SSLHRNHMPSYWGVYYPTAWDWSILAGSIGLFLTMFFTLLRIVPIVAMSEVREIIDKG
                     GPR"
     gene            11804..12343
                     /locus_tag="VKU03_00560"
     CDS             11804..12343
                     /locus_tag="VKU03_00560"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3341 domain-containing protein"
                     /protein_id="HLJ69776.1"
                     /translation="MSNGEPLGLVGVFASPQPMVEAARQLHALGIEAVEAYTPYPVEA
                     LGEQAHAGRPTALPWIMAAGALVGACCGYFIQYWDEWLNYPLNVGGRPYNSWPAFTVG
                     AFEVTLLFAVTAGFFGLWFACRLPRLYDPKVEDEVFDRASTDRFVLRVETGASNVPRD
                     RIRRVFEQHGAEQIVEVAA"
     gene            12340..12852
                     /locus_tag="VKU03_00565"
     CDS             12340..12852
                     /locus_tag="VKU03_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLJ69777.1"
                     /translation="MTRRPALLLLALALGLAACDNMANQRKRMPYEMPYGAEAANWPA
                     QPPDGIVARDEKPVAPPPVTMALLKRGQERFGIYCTPCHSLLGDGDGMIVHRGFPRPP
                     SYSIQRLRDAPAQHFYDVITHGYGVMFPYAARVAPADRWAIVAYIRALQASTDGRLAD
                     VPADQRGSLQ"
     gene            12849..13940
                     /locus_tag="VKU03_00570"
     CDS             12849..13940
                     /locus_tag="VKU03_00570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69778.1"
                     /translation="MNGEWNWRRLNLAALAAFVAGAIGCGVGAALDLDGFSRAWLCAF
                     LFWLGLPICGVTLVLVHDLTGGRWMASARPALNAAIATMPLAALAGIPAFVRIAALYR
                     WAHPGQSLSNAFYLNSTAFYLRYAIDVVLWCAIAAFALWGPRGAAAPIAPARSWISAV
                     GLIALAFSASFASIDWVLSLEPTFWSSIFPMIVGAGWFNTGLALVLLVVAAAGVERDH
                     MADIAAILLGVVIFWAYVEFMQFLIIWEENLKSEIPWYLPRLSGVWAPTALVGLALGF
                     FVPFFVLVSRPGKRSRAAVMIVCAAILLSRLADHWWLVLPEFSPGGPFWLDVAAIFTL
                     GGLMLLLFFAGLRSQGLAVSAATLARDHG"
     gene            13933..14307
                     /locus_tag="VKU03_00575"
     CDS             13933..14307
                     /locus_tag="VKU03_00575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69779.1"
                     /translation="MAESEPRVAANPNAAYETSDWPLRPVAWTYASGLILLAIAVGAM
                     LWAYPHTLGDVSRKRGVEPPAPRLQIDPAEDLAAFRADKERKLNSYYWIDKKNGVVHI
                     PIEQAMEKIVKSGIDGFPKAQP"
     gene            14304..15089
                     /locus_tag="VKU03_00580"
     CDS             14304..15089
                     /locus_tag="VKU03_00580"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLJ69780.1"
                     /translation="MMRAFSVPILATMLAFGAAIHPAAADDFSDLAFDPRPGAQLPLK
                     EVFIDEQGRMTALGNYFTGKPVVLVLDYLRCKALCGVTLEHLTAALGALPLELGRDYQ
                     LVGISIDPRDKPIDAAAAKAKYLALYGHAGGESGAHFLTGDGAGARRVAAAIGFRYRY
                     DAALDQYIHPAGFVIATTDGRISRYMLTVGPALATLQAALGDAAAARVTDPLTRFLLL
                     CHVEGAPLGRYDGPILVAFSLANLAAMAALIVLFGTIRRRRNG"
     gene            15095..16051
                     /gene="coxB"
                     /locus_tag="VKU03_00585"
     CDS             15095..16051
                     /gene="coxB"
                     /locus_tag="VKU03_00585"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HLJ69781.1"
                     /translation="MRHWLPFWPLISAKQGVVVNNLVIAEVAVSALTVLAVFLMMVTF
                     CIRYRRGSQASRAHLREKTWRIEIGWTTATLIGFLALFVWGAGIYVWLFQSPPGDIEI
                     YVVGKQWMWKFQHPGGQREIDALHVPAGKTIRLVFSSQDVIHSFYIPAFRIKHDVVPG
                     TYETLWFNTTKTGVYRLECTQYCGDQHATMTGEVIVMEPTAYEKWLTDQGVHQSLAEQ
                     GEALFRQYGCSGCHGANSTVHAPSLAGVYGSMVQLQDGSVRRADEAYLRDCILTPRTF
                     TVAGYPPIMPNFSGQIGEDDLMKLIAYIQSLAQGARHGDDRR"
     gene            16032..17669
                     /locus_tag="VKU03_00590"
     CDS             16032..17669
                     /locus_tag="VKU03_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HLJ69782.1"
                     /translation="MAMIAAEIEPTYLDNEHTIASWLLTKDHKRIAMMFLVSITVFFF
                     IGGIAATLIRLHLATPQGLLQPSLYNRMFTMHGVIMVWFFLIPSIPTTLGNFLVPMMI
                     GAHDLAFPRINLASWYVFNLSGLVLLYALIMGGVDTGWTFYTPYSTVYSNGYVFAAAL
                     AVFVNGFSSIMTALNFVVTIHRLRAPGMTWARLPLFIWGIYAVSVVMLLATPVLAMDT
                     MLLGVERFIGLGMFDPRRGGDPILWQHMFWFYSHPAVYIMILPGMGLVSELIACYARR
                     PIFGYAGMTIAMMAIAILSFFVWAHHMFVAGISIYSAIVFSLTTFFVAVPSAIKVFNW
                     TVSLHKGAIIFDAPMLYALGFVLLFTIGGVTGLMLATLGVDVATHATYFVVAHFHYIM
                     VGATVSAFFGGVHFWWPKMTGRLYPEMWARFAAILTFIGFNVTFFPQFILGNLGMPRR
                     YYTYPPEWQSWNLLSSAGASILAVAYLLPLFYLGWSLVYGKRAGPNPWRATGLEWLTP
                     SPPPKDNFERTPVVTIGPYSYDPETDHYITQDGVSHA"
     gene            17662..18306
                     /locus_tag="VKU03_00595"
     CDS             17662..18306
                     /locus_tag="VKU03_00595"
                     /inference="COORDINATES: protein motif:HMM:NF012720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HLJ69783.1"
                     /translation="MPSATLRPETQYATLAQQGEVAQLGMWVFLATETLFFGALIFTY
                     YLYRLSYPEAIAEAAKDAVLWCGSVNIGLLLTSSLTMVLAINAAAQGRRGAATGWLLT
                     TIGLGLCFLGVKGYEYVADYSDHVVPAVNFALKPGESPVSELFWVYYFVATGLHAIHV
                     TTGLGLLIYILWRLRRGRISAAYYAPIEVVGLYWSFVDTVWVFLYPTIYLAGRT"
     gene            18303..18602
                     /locus_tag="VKU03_00600"
     CDS             18303..18602
                     /locus_tag="VKU03_00600"
                     /inference="COORDINATES: protein motif:HMM:TIGR02229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oxidase"
                     /protein_id="HLJ69784.1"
                     /translation="MNIEAKEASSFPFVCAWLLLLILVGLSVLSAFQGLALWAAVIEF
                     GIAAFQALVIFLLFMRLKGPPSLKWVFAGTGFFWLIFLYGLSMTDYATRTGWPTK"
     gene            complement(18616..19179)
                     /locus_tag="VKU03_00605"
     CDS             complement(18616..19179)
                     /locus_tag="VKU03_00605"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016552403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLJ69785.1"
                     /translation="MSQAAQRYFVFETAAGFCGLAWSQSGITRFQLPTRSAEATERLL
                     RRRTPGAEPAAPPPEILEAAAAVKSYFEGEETDFSRFDLDLADQDDFFQRIYAAARQV
                     GWGRTTTYGALAKALGAGPEAARDVGVAMAKNPVALIIPCHRVLAAGGKIGGFSAPGG
                     AATKRRMLELEGVRLEPPRPVQAAFEF"
     gene            complement(19333..19725)
                     /gene="rplS"
                     /locus_tag="VKU03_00610"
     CDS             complement(19333..19725)
                     /gene="rplS"
                     /locus_tag="VKU03_00610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L19"
                     /protein_id="HLJ69786.1"
                     /translation="MNIIETLEAEQAAKLAQTSKVPEFQPGDTVVVNVKVKEGERTRV
                     QAYEGVCIARSGGGLNESFTVRKISYGEGVERVFPIYSPMIDSVKVLRRGKVRRAKLY
                     YLRDRRGKSARIAERSEGPAGKKDEAKA"
     gene            20141..20713
                     /locus_tag="VKU03_00615"
     CDS             20141..20713
                     /locus_tag="VKU03_00615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HLJ69787.1"
                     /translation="MAPAAAQATIPMDQLMAPGPLPDIVQGKADAPVTIIEYASMTCT
                     HCAAFHAETWPALKAKYVDTGKVKFILREFPLDPLATAAFMLARCAGPDKRNAIVDLL
                     FDKQKDWAFVDKPLEALANTVKQAGISQGDFESCLKNQQLYEQVNQTRDNAAKNFHVD
                     ATPTFFINGKKLSGERPLADFDKIIQPLLK"
     gene            21049..24507
                     /gene="smc"
                     /locus_tag="VKU03_00620"
     CDS             21049..24507
                     /gene="smc"
                     /locus_tag="VKU03_00620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosome segregation protein SMC"
                     /protein_id="HLJ69788.1"
                     /translation="MKFDKLRLAGFKSFCETTDFKIESGLTGIVGPNGCGKSNLVEAL
                     RWVMGENSYKNMRASGMEDVIFSGSGNRPARNIAEVGLTLDNTDRQAPAAFNDADIIE
                     VTRRIEREAGSTYRVNGREVRARDVQLLFADASTGARSPALVRQGQIGEIIAAKPQAR
                     RLILEEAAGVSGLHSRRHEAELRLKGAEENLVRLEDVLAQIDQQIESLKRQARQATRY
                     KGLAAEIRRVQALALHISWRDEEQRFAEAQRRLEADTRDVAERTADQAEAARVQGIAA
                     AALPPLRDEEARAGAALHRLVLAREALDGEERRAKERTAELERRVAQLAQDAQRETAL
                     IDDAAATLERLAQEDAALAEQQGASETARQSAEARLAEAEQLLAACEARLNEAQAALS
                     DLNARRNALSAALAEETRRLSRFEAECVSIAQERDKLVAEAGGLNEQSEDAAALEALN
                     ARLAAAEARTLAAEAAQAEAREAEQRSRGPLADAERSAQRLETEARTLMKLLGSDSGG
                     LWPPVVESLSVEKGYEAALGAALGDDLDASTNTSAPAHWTELDGSFEPAPLPPGVQSL
                     ASLVKAPPALQRRLSQIGVVLRGEGRALLPLLKQGQRLVSKEGDLWRWDGFVSAAEAP
                     TPAARRLAEKNRLGDLTRDAEAARQAAEALKAQAVAAQAALREAASAEQAARHEAREA
                     RRLVDAARERLAALERRRAQTAARLSALEEAASRASASRDEAAMRKSEAEAALAGLVA
                     TDHLALDLERARGEATKARAECAEARAASQNLAREAAARAARRQAIAEDQKSWSQRRE
                     RAEVQIAEFAARQSEATRELEPLADAPAAFLQRRRALISEIDAAEQARKKASDARVAA
                     ETALSEADRAAKAALEAMSAAREARAASEERLEAARQRRENLLHTIAVELETEPHALA
                     GLAGVKADEDLRELAQVERKLENLKQERERLGAVNLRAEDELVEVEASRSRLIAERDD
                     LIEAIKRLRVAIGSLNKEGRERLVAAFDVVNKHFGELFATLFEGGSAYLQLIESEDPL
                     EAGLELIAKPPGKKPQTLTLLSGGEQALTAMSLIFAVFLTNPSPICVLDEVDAPLDDS
                     NVERFCDLLEEMRKRSDTRFVTITHNPITMARMDRLFGVTMAERGVSQLVSVDLAEAE
                     RFLEAS"
     gene            complement(24516..24854)
                     /locus_tag="VKU03_00625"
     CDS             complement(24516..24854)
                     /locus_tag="VKU03_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012709483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ69789.1"
                     /translation="MSTRVTLRLDFDAGRRLGPGKVALLEAIRDTGSISAAGRAHEMS
                     YRRAWLLVEELNTMFAEPLVTARPGGAKGGGAALTEAGERLVALYREAERKTRASASA
                     EIARIEKGLA"
BASE COUNT         4019 a         8480 c         8086 g         4686 t
ORIGIN      
        1 gtcatcaagc aggccgcgga taagggcatc gtcgtcgtcg cggtcgatca ggcggtctcg
       61 gcgcctgaag cctatgtctt gaccaacgac caggtcgcct acgccaaggt cggcgccaaa
      121 tggctgttcg acaagctcgg cggcaaaggc aatgtcatcg agatgcgcgg cattgacggc
      181 gtgccggccg acgccgatcg ccataatggc ttcgagcagg tgctgaagga ttacccgaac
      241 atccacgtca cctccgtctt caccggctgg gctttgaaca agacggcgca agagaccaag
      301 gaccttctcg cctccggcaa ggagatcgac ggcatctgga cctccggcat cgattccacc
      361 gtggtcgacg cctaccagac ggcgggtaag ccgttcgtgc cggtggtcgg ggccgacaat
      421 aacggcttta ttggccagtt gatcaatctg aaggacaagg gcctgaccgg cgccgcggtt
      481 agcaacccgc cggctgtcgg cggcgccggt ctcggcctcg ccctcgatgt gctgcagaaa
      541 aagccgcatc cgcatgtcgt gaagatcacg ccgacggttt gggacaatac cagcgcggcc
      601 ggcatggagg agctcaagaa ggcttacaat cccaagcttg atcccttcta cagcgtcgcg
      661 atcaacgtgc cgccctacac gcattacacg atgcagcaga ttctggcttg caaggggccg
      721 tgatcctcgt attcgtgtag gatgtttctt tacggggccg gcggcgaaac cgccggcctt
      781 tttgagcctg cctgatgtct ccggaccaag attcgatctt gcatataagc ggcgtcgcca
      841 agcggttcga ttccgtggtc gcgctgcgct ccgccgaatt gaaggtgcgc gccggcgagg
      901 tccatgcgct gatgggcgcc aacggcgccg gcaagagcac cctggtcaag atcatcaccg
      961 gcgtgtttcc ggccgattcc ggcgacatcg tgatcgccgg cgagcgcaag gttttccgct
     1021 cgccggcgga ggcgcgccag gccggcatcg tctcggtcta tcaggatccg gccatcgttc
     1081 cggatttgac cgtcgcccag aatatgcgtc tcgcccgggt cgggctcgac gccgtgcgcc
     1141 agtcgatgcg cagcctcggc gtcgagcgga tcgatctttc ccgcgccgcg cgcgagatcg
     1201 acttcgcgac cttgcgcctg atcgaccttt cgcgcgcgct cgcctccaat ccgcgcatcc
     1261 tgctgctcga tgagatcacc gcggcgctgc cgatcgatct cgccgagcga gtcttcgccg
     1321 tcgtccgcga ctggcgccag cgtggcgcca gcatcatctt catttcgcat cgcatggccg
     1381 aggtcgcggc gctttgcgac cgcgcgaccg tgctgcgcga cggcgtcacg gtgggcgtga
     1441 ccgcgaccaa aggcgccgaa gacaagatcg tcaacatgat gctcggcgcc gaggtggcga
     1501 aagcggcggt atcggccggc gcgcgcgcgc cgaaggcggc gctcgccgct gcgtcggcgc
     1561 tcgaagtgaa ggaccttgcc tgcggccatg gcctgcgcgg cgtctccttc gccgtgcggc
     1621 ccggcgaggt tttgggagtc gccgcgctcg aaggccaggg tcaagaagag ctcttcgatt
     1681 gcatcgccgg cgtgcgccgg gcggacgccg gggagatatg ggcggacggc aagccgctgc
     1741 gcgcgcgcca tcccgccgac gccgtacgcg ccggtctggt tctggtgccg gccaatcgcc
     1801 tgcaagcgct tctgcagcag cgctcgatcc gcgagaacgt cgccttgccg attttcggcc
     1861 gggtgaagaa ctgggggccg atcgcgatga aggacgagcg gcggcgggtc gacgacgcgg
     1921 tccgccgcct ccagatcgac acccgcgcgt cgtccgagct gcgccgcctc agcggcggca
     1981 atcagcagaa agtggtgatc gcgcgctggc tcgcttccgg ctttcgcacc ttgctctgct
     2041 tcgatccgac gcgcggcatc gatgtcggca cgaagcggca gatctacgaa ttgctccgcg
     2101 aggtggcgcg gcaaggcgcc tcggtgcttc tcttcacctc ggagctgccc gaaatccgac
     2161 tcgcctgcga tcgggccatc gttcttttcg gcggccgcat ttccggcgaa atgaccgccg
     2221 acgaggcgga cgaggcgcgc ttgctgcgcg ccgcccatgg cttgaccgcc gaagcggcgg
     2281 aggcgcgcgc gtgagcgagg cgagcctgcg cgaatccggc ggcctcgtcc gcatgttgag
     2341 ccggcgctcg cgcctgctcg gcatgccggc gctgctggcc gcggcgattc tcgcgacggc
     2401 gttcatccat cccgatttcg gctcgttcga cattcagtcg ctcgcgctcg gcgccttgcc
     2461 gctggccatg gccgccgccg cccaggcgat cgtcgtgatc agcggcggca tcgatctttc
     2521 cgtcggctcg ctgattgcgg tctcgaacgt gctggcggcg agcctgatgc agcatgcctc
     2581 gttcggcgcc tcgctcggca tcgccgtggc ggtcctggcg ctgggcgccg cgcaaggcgc
     2641 gatcaacggc ctgaccgtcg cgatctcgcg cattcccgat gtcgtcgtca ccttgaccac
     2701 cggcttcatc tggggcggcg tggcgctgct cattctggag cggcctggcg gcggcgcgcc
     2761 ggcggcgttc ctcaacatcg ccgcgggcgc ggacgtctcc gcctggatac cgaacgccct
     2821 gatcgtcatg gtcgtcgtca ttgcggcgat ttggctgccg attaagctca gccggatcgg
     2881 cctcttgctc tacgcctcgg ggagcgatcc ggtcgccgcc tttcgcagcg gcgtcgatac
     2941 ccgcctcgcc cgcacgctgg cttatacgtt cggcggcgtc gcctgcgccg ccggcggcct
     3001 ttcgctgacc atgacgaccg gcatcggcgc gccgctcgcc ggcacgtact atactttgag
     3061 cagcgtcgcc gccgcggtga tcggcggcgt cagcctcaat ggcggacgcg gcggcatggt
     3121 cggcccggtc atcgccgctt tgctgctgac gctcattccc atggatctgg tcttcctcgg
     3181 catcgatccg aatttcggcc aggtcatcca gggcacgctg atcgtgctcg tcgtgatgat
     3241 cggcggcttc gtcactttcg tccagagccg caaatgaacg ccgcactgca attcctgaaa
     3301 accaggacga ttgtcgcgct catcctgctg ctcgcgatct ttctgatcgg catcgagatc
     3361 gtgcggcccg gcacggtgac gccgctctgg ggttcgaacg cgctcctctt cgccgcgccg
     3421 ctggcgatta tgggcgcggg gcagacgctc gtcatgctca ccggcggcat cgacctgtcg
     3481 gtggcgagcg tcgcgacggg ggccgcctat cttctggcga ccaacgcctc gtcgggcgat
     3541 tcattcgcca tcgtcatcgc tttggcgctt gggctgatcg tcggcgtcgt caatgggatc
     3601 ggcgtcgccg tcctgcgcgt ccagccgctg gtgatgacgc tcggcaccgg cctgatgacc
     3661 ggcggcgtga tgatcgtcta cagccagcaa atgctggcga gccagccgca tgtgccggcc
     3721 gtcattcaga cgctcggcgc cggcaaggtg cttggcctgg tgccggtcga tcttttcctc
     3781 tggatcgtga tcgccgcgtt gatgctttac ggcctcaacc ggaccggctt cgggcgcttg
     3841 atctacgccg tcggcgacaa tcgcgaagcc tgccatctcg ccggcattcg cgtctggcgg
     3901 gtcctgttcg tcgactacct gctctgcgcg ctcctggccg cgatcgccgg ccttgtcctg
     3961 gtcggcggga ccaacgccgc cgacctcagt ctcgccgacg cgtatctgct gccctccgtc
     4021 gcctgcgtga tcatcggcgg cacgtcgatc ttcggcggcc gcggcggcta ttccggcacg
     4081 atcgtcggcg cgctgatcct caccgccctc accagcctct tgacgctgct cgacgtcccg
     4141 gagccggtca agcaaatcct ttacggggcg atcatcctgt cgctcgccgc cgcttatgcc
     4201 cggctcaccg aatgagcgcg gcgcaatttc ctgatccgat ggcgtggatc ggaactttcg
     4261 cacggcctac gtcgtagtaa ggcgcataga cgctgcgaaa cggcctcagc ttggcctctg
     4321 tcgcatcgcc gcgctcggga cgcaaggcga tgcgcggctc cgaaggagtc ggctcgatct
     4381 gtggcggcgt cgcaatgggc gtgtcgccat gaagccgagc atcgtcaatc gcctcagcgt
     4441 cgatccggat aagatcataa tttttcgcgg cggccacgtc gtgatcgcca actggcgcgg
     4501 gctgatcgtt tcgcaggacc aaggctacta cgtccatcat acgcgcttcc tctcgcgctt
     4561 cgatttgcgc cttgacggac gcagcgtcca gccggtctgc tgcaaccgca tcgcgccgca
     4621 tgccgcgatg ggctactacc ttgcgccgtc gccggccggc cgcgaagcgg cgccgcccgg
     4681 cgacgacgat ccgagcggcg gcgaagtcgt ccagaaagga atagagatca aggtcaggac
     4741 gtatgccggg ggcggctgtc atcaggacat cgtcttggtc aatcatgggc tcgcgccggc
     4801 gacaacgtct ctggatttgg tttttgcggc cgattttgcc gatcttgaag gggtcacggg
     4861 cggcgagcgg cggcaggatg ccccgatccg ccgcaacttc aaggagctgg gccacggccg
     4921 cggcgaactg accttcagct acgagcatcc gaagctcaac catgcgacgc ggatcgtcgt
     4981 cgccggccct ggcggcctcg tcgacgacgg cgctgcgctt cgcataacgc tggcgctgcg
     5041 cccgcaggcg cccgaaacga tcgccgtcga tatcgcgccg atctttctcg gcgaacagat
     5101 ccagccctgg ttcgggatcg gcggcgagcc gaccgcgcat cacccggatc aagcccgcct
     5161 cgagcgctgg ctggacggat gcgtcgaggt cgcattcgcg aaccgcgcgg tccaggccgc
     5221 atggaatcgc gccgcaacgg acctctggtc gctgcagggc ctgcgcggcg agggggaaga
     5281 actcttcacg ccgatcgccg gcatcccgaa atataccggc cttttcggcc gagacgccct
     5341 ggtcgcggga ctgcagtcgg ccatgctcaa cccctcgacc ttgcgcggaa cgctgtcgac
     5401 tttggcgcgc tgggcggcaa aaaccctcga cgaccgcttc gacgccgagc cgggcaaaat
     5461 attgcaccag cgccagctcg gccctctggc gctgctcggc aagaatccat tccttcatta
     5521 ctacggcgac tattctgctg ccggcctctt cgtcatcggc gcggcgctgc acttcgccca
     5581 ttgcggcgat cgcgacgcgt tcgaggcttt tcgcaatccg gtcttggccg cgctcgaatg
     5641 gatggatcgc gacggcgaca aagacggcga cggattctac gaataccaaa cccgggccgg
     5701 cgattccggc atcaagaatc aaggctggaa ggactcgaac caggctattc tctatcccga
     5761 cggctcgttc acgcccaacc ccgtcgcggt tgccgagatt caagggatct attacgccgc
     5821 caagcagagc atggcgtgcc tcttcgcgac gatcggcgag cgcgaggcgg ctgacaaact
     5881 cttcggacag gcggccgcgc tcaagcggcg gttcaacgag cgcttctgga tggaggacct
     5941 cggctttttc gcgctggcgc tcggtccgga caagaagccc gtcaagacga tcgcgtcgaa
     6001 tgtcggctca tgccttgcct atgggatcgt cgacgaagac aaggcgaagg cggtggcgga
     6061 tcgcctgctg gcgccggaca tgttcagcgg ctggggggtg cgcaccctgt cgagcgagca
     6121 tcccgccttt aatccgcttt cgtaccatct cggctcgatc tggccggccg ccaacgccaa
     6181 tatatgcttc ggcctaaagc gctatgggtt caacgacgcc tgccataaaa tcgccaaggc
     6241 gattttcgat gctgcgggct tgtttgagca tgatcgcctg cccgaagtgt tcggcggtca
     6301 tccgcgcgac aaggatcatc cgcatcccgg cgtctatccg gaatcgtgtt ggccgcaagc
     6361 ctggtcggcc tcggcggtca tccaaatttg tcacgccttg gccggcctga cattcgccgc
     6421 gcctctcaac gcgctggtgc tcgatcccgc tctgcccgat tggctgccga agatgacgat
     6481 cgacaacctc atcgtcggcg gcaagcgcgc gaaaatcgaa gtcgagcgcg acgcgagcgg
     6541 cgccgtggcg catcgcgtcg tggaagcggc ggacttgcgc atcgtgagat tagaccccga
     6601 tttgccgccc ggtaaagatc ggcttgcgca cgccgccaaa aagctcgcgc aaagtcaaat
     6661 ggcgtcaaac ccggtttaag cgaatgcgcc gtcgctgctg tggcgcggac gccgttttgc
     6721 agcgtcagat tgacgcattt gaaaagcttc aggcgaattt tccaattttt ggcggaacaa
     6781 agcgccgcgc gaacccgttc gggcaaccgt ccctgcaata attgcgtttc ccgatcagcg
     6841 ctgatcgacg ggggcgccgc ggcggagtga tccggtggcg gtgttcggtc cgagggcgaa
     6901 tttagcggtc aacgcggcgc tggtcggcgc ggtggggctg cttctggtcc tgctcctcat
     6961 gatctgggtg gttccggtga cgggttacaa caccctggag cggtacacgc cgatacagcc
     7021 cgtccctttc agtcacaagc atcacgtcag cggccttggg cttgattgcc gctattgcca
     7081 tacgacggtc gagacgtcgc agaacgccgg cattccgccg accaagacgt gcatgacctg
     7141 tcattcccag atctggacca atgcggcgat ccttgcgcca gtacgtcaaa gcctcgctga
     7201 cggcaagccg ctgcgctgga ccagggtcta cggtctgccc gactacgtct atttcgatca
     7261 cagcatccat atcgccaaag gcgtcggctg cacggaatgc catggtccga tcggcgacat
     7321 gccgctgacc tggaaggcga cgacgctcta catgagctgg tgtctgtcct gccaccgcga
     7381 tccggcgccg catttgcggc cgctcaatga agtgttcaac ccgcattggc gccggtcgcc
     7441 cgcgacgccg agcggcgctg aactcatggc gacctatcac atccataccg aaaccttgag
     7501 cgattgcggc acatgccatc gatgagcgca gccggcaaag cctattggcg cagccttgag
     7561 gacctggcgg aaagcccggc tttcgccgcc gcgatcgagc gcgaagcgcc gcgcctgcgc
     7621 gatctcgtca attcagtgga ccgtcgccgc ttcctgcaat tgatggcggc ttcgatcgct
     7681 ctcagcgggc tgaccggctg cggtcccgag gccaatctgc gtcagctctt gccctatgtg
     7741 gagcagcctg aaggaatcgt gccggggcgg tcgcgctatt acgccaccgc gacgacgctg
     7801 gaaggctatg caaccggcgt gctcatcgcg catgaaatgg cgcggccgat caaggtcgag
     7861 ggcaatcccg atcatccggc cagcctcggc gcagccggcg cgatcatgca agcgaccatt
     7921 ctcagccttt atgatccgca gcgcgcgcag tcgattgtcg gcaatcgtca aatcgacacg
     7981 tggcagggat tcatcgcggc gctgcacgac cgtcgcgccg cgctcggcca aactcatggc
     8041 gagggcctgc gcctgttgac cggcgcggtg tcgtcgccct cgctcgtcgc ccagatcgcg
     8101 gatttgcaga aacagtttgc cgggatgcgc tggcatcaat gggagccgct cgcccgagac
     8161 aatcaatgga cggctctgca acaggcgttc ggccgtccgg tcgaaacgat tttcaggctc
     8221 gatcgcgccg atgtggtttt cgccgtcgaa agcgatctca tttcctcgac gcccggctgg
     8281 ctggcctatg cccgccaatt cgcagcgcgg cggcggccga ccgagacgaa cggcgcgatg
     8341 agccgcgtct acgccatcga gagtacgccg accttgatcg gcgccaaggc ggaccatcgc
     8401 ctcgctttgc gcccggcgga aatcgccgcg gcgttgcgga tcgtcgccgg aacggtcggc
     8461 gccgggccga aggactggat cgccatcgag gccgccaacg ccgattggtt caaggcggcc
     8521 gccgaggatt tgatccagca taagggcagg gcgctcgttc atgtcgggcg cgagcagccg
     8581 ctcgaaatcc atcagctcgc agcggcgatc aacggcgcgc tcggcgcgtt cggcggcacg
     8641 atcggcgtga tcgagccggt catcgcctcg ccagcgccgc atatccaatc gctgcgcgac
     8701 ctcgtcgacg acatgaaagc cgggcgggtc gacacgctca tccttatcga caccaatccc
     8761 gtctatgccg cgccggccga tttcgaattc gccgacgcgc tcgccaaggt caaattctcg
     8821 gcgagccttt ccctctatgc cgacgaaacg gcgcatgcct gtctttggcg cgtgccgctc
     8881 gcgcacgaat atgaggcctg gagcgacgcg cgcgctttcg atggaacgat tacgatccag
     8941 cagccgcagg tgcgccgcct ctatggcgga cattcggcgc aagaggcgct cgcggcgctg
     9001 caagggaaca cccagccgga cgatcataag ctgctgcgcg aattttggcg gggcaaagcg
     9061 caacaggaga atcgcggcga cttcgagcag ttctggcgcg actcgctgcg cgccggtctc
     9121 gtcgccgaca gcgccgccgc gccggtcacg gcgccgctca agtccgatct cgccggcgcc
     9181 gctccggctt ccacgccgaa cgcatcgctg atcgagctcc tgttccggcc cgatgaaagc
     9241 ctttgggacg gacgtttcgc ggacaatgcg tggctgctcg aaacgccgcg ggccttcacc
     9301 cggttgacct gggacaatgc ggcgctgatc gcgcctcaga ctgcgaagcg ccttggcctc
     9361 gccgacggag acgtggtcga aatcgcgacg caacacggca agctgaaagc gccggccttc
     9421 gtcctgcccg gccaggcgcc ggactgcgta acgttgccgc tgggcttcgg ccggcgcgcc
     9481 ggcggcctca gcgtcgggca gggcttcgac gcctattcca tacgcggcgc cggagcgcca
     9541 tggcttgcgc cagcggcgtc gatcgtcaaa accggcgaca ccgcgagatt ggcgacggtg
     9601 cagggccacg accgcgtcgc cggccgcgac ctgatccgcg aaggcgatct tgccgacttt
     9661 cggcaaaacc cggattttct gcagcgcaag acgagcgagg aatcgcttta tcccggctac
     9721 gactatcccg gccgggcctg ggcgatggcg atcgacctca attcctgcat cggctgccag
     9781 gcctgcgtcg tcgcttgcca ggccgagaac aatgtgccga ccgtcggcaa ggacctggtc
     9841 atcggccagc gcatcatgca ttggctgcgg atcgacaggt actattccgg cccggccgac
     9901 gcgccctccg tcgccttcga gccgatgccc tgcatgcatt gcgaaaacgc gccttgcgag
     9961 gtcgtctgtc cggttcatgc gacgacgcac gatcatgaag gcttgaattt gatggtctac
    10021 aaccgctgcg tcggcacgcg gttctgctcg aacaattgcc cctacaaggt gcggcgattc
    10081 aatttcttcg ccttcgccga tcgcaccgac aggccgcccg aatcgtggaa tccgcaggtc
    10141 tcggtgcgcg gccgcggcgt catggagaaa tgcacctatt gcatccagcg catccgcaac
    10201 gccgagatcg acgccgaccg cgacaatcgc cccttgcgcg acggcgaggt cgtcactgcc
    10261 tgccagcaga gctgtccgac ccaggcgatc gtcttcggcg atcgcaacga tcgtggcagc
    10321 gcggtcgcca agcgcaaggc ggcgccgacc gattatacgc tgcttgacga gctcaacacc
    10381 cggccgcgga ccagctatgc ggcgctgatc cgcaacccaa atccgcgcat cgagggcgag
    10441 aagacatgac cctcgccgcc gacgtcccgc gtcccgctcc ggtcgtcgag ccgggcgtca
    10501 cgctgcgctc gctgagcgag caggtcagcg gccggctgct gagccggcgc ccgccgcttt
    10561 ggtggtgggt cggcttcggc tgggcgctgg cgctgctgct tgtgctgatc gtctcggtga
    10621 ccaatctgtt cgtcaccggc atcggcatct ggggggtcaa cattcccgtc gcctggggcc
    10681 tggcgatcgc cgaatatgtc tggtggatcg ccttggcgag cggcgggaca atcgtctccg
    10741 cgctctttca tgtgacccgg tcgccttggc ggtcagcgac caatcgcatt gccgagacca
    10801 tgctgctttc agcggcggcc tgcgccggca tcatgccgat cctgcatctc gggcggccgg
    10861 gccttttcta ttggctgttc ccgtattcca cagtgatggg ggtatggccg caagtgcgca
    10921 gcccgctgtg gtgggacttc gtctgcctcc tttgctacat cctgatgtcg atcatgtact
    10981 attatgtcgg cctggtgccg gacgtcgcca ccgtgcgcga cctggcgcag agccgatcga
    11041 aaaaagtttt ttacggcgtt ctcgccatgg gctggcgagg ctcggcgcgg cactggcgca
    11101 agcacaagac cgtctatgcg atcatgtcgg cgatcatggc gccgatggtc atctccgttc
    11161 atagcgtggt cggcctcgac ttcgccgccg gtctgacgcc gggctggcac tccacgcaat
    11221 ttccgcccta ctttttcttt ggcgccgtga tttccgggac cgcgctcgtc gtcatgctga
    11281 cgatcctggt gcgttggggc tacgggctcg aaaacgtcat caccgactat caccttaacg
    11341 cgctcggcaa gctcgtcctc gtcggcagcg tgatgctcgg ctacgcttat ctgttcgagg
    11401 cttgggggcc gatctatggc gccgacgccg ccgacaagac cgagttcgcc tcaagagtct
    11461 tcggattcac ggcgggcgcc tattggggca agatcgccct caacatcctc atcccgcaat
    11521 tgctgtggct gcccgccatc cgccgcagca aggcggctct gttcctgatt tgcgccggca
    11581 tcatcgtcgg catgtggctc gagcgctaca tcatcgtggt gtcgagcctg catcgcaacc
    11641 acatgccgtc ctattggggc gtctattatc cgacggcgtg ggactggagc attctcgccg
    11701 gctcgatcgg cctgttcctc acgatgttct tcacgctgct gcgcatcgtg ccgatcgtcg
    11761 ccatgtccga ggtgcgcgag atcatcgaca agggcgggcc gcgatgagca atggcgagcc
    11821 gctcggcttg gtcggcgtct tcgcttcgcc gcagcccatg gtggaggcgg cgcgccagtt
    11881 gcatgcgctc ggcatcgagg cggtcgaggc gtatacgccc tatccggtcg aggcgctcgg
    11941 cgagcaggcg cacgccggcc ggccgaccgc tttgccttgg atcatggcgg ccggcgcgct
    12001 tgtcggggcg tgctgcggct atttcatcca gtattgggac gagtggctga attacccgct
    12061 caatgtcggc ggcaggcctt acaatagttg gccggctttc acggtgggcg cgttcgaagt
    12121 caccttgctg ttcgccgtca ccgccggttt tttcggcctg tggttcgcct gccgcctgcc
    12181 gcgcctttac gatcctaaag tcgaggacga ggttttcgac cgggcctcca ccgatcggtt
    12241 cgtccttcgg gtcgaaaccg gcgcgtcgaa cgtcccgcgc gacaggatcc ggcgcgtctt
    12301 cgagcagcat ggcgccgagc agatcgtcga ggtggccgca tgacccgccg gccggcgctc
    12361 ctcctgctcg ccttggcgct tggcctcgcc gcctgcgaca acatggccaa tcagcgcaag
    12421 cgcatgccct atgaaatgcc ttatggcgcg gaggcggcga actggccggc tcagccgccg
    12481 gacggaatcg tcgcgcgcga cgagaagccg gtcgcgccgc cgccagtgac gatggcgctg
    12541 ctcaagcgcg gccaggagcg cttcggcatt tattgtacgc cgtgccacag cctgctcggc
    12601 gacggcgacg gcatgatcgt ccatcgcggc tttccgaggc cgccttccta ttccattcag
    12661 cgcctgcgcg atgcgccggc gcagcatttc tacgacgtga tcacccatgg ctatggggtg
    12721 atgttccctt acgccgcccg ggtggcgccg gcggaccgct gggcgatcgt cgcctacatc
    12781 cgcgccttgc aggcgagcac ggacggacgc ctcgccgatg tccctgccga tcagcgcggg
    12841 agcctgcaat gaacggcgaa tggaattggc ggcgtttgaa cctcgccgcg ctcgccgcct
    12901 tcgtcgccgg cgcaatcggt tgcggcgtcg gcgcggcgct cgatctcgac ggttttagcc
    12961 gcgcatggct ctgcgccttc ctgttttggc tcggcctgcc gatctgcggc gtcaccctgg
    13021 ttctcgtcca tgacctgacc ggcgggcgct ggatggcgag cgcgcggccg gcgctcaatg
    13081 cggcgatcgc caccatgccg ctcgccgcgc tggccggcat tccggctttt gtccgcattg
    13141 cggcgctgta ccgttgggct catccgggac aaagcctcag caacgctttc tacctcaact
    13201 cgaccgcctt ttatttgcgc tacgcgattg acgtcgttct gtggtgcgcg atcgcggctt
    13261 tcgcgctgtg ggggccgcgc ggcgccgccg cgccgattgc gccggcgcgc tcctggatca
    13321 gcgccgtcgg cctgatcgcg cttgctttct cggcgagttt cgcttcgatc gactgggtac
    13381 tgtcgctgga gccgacattc tggtcctcga tctttccgat gatcgtcggc gccggctggt
    13441 tcaataccgg gctcgccttg gtcctgctgg tggtcgcggc ggccggcgtg gagcgcgacc
    13501 acatggccga catcgccgcg atcctgctcg gcgtcgtgat tttctgggct tacgtcgaat
    13561 tcatgcaatt cctgatcatc tgggaggaaa atctcaaatc cgaaattccg tggtacttgc
    13621 cgcgcctgtc cggcgtctgg gcgccgaccg cccttgttgg cctggcgctt ggatttttcg
    13681 ttccgttctt tgtcctcgtc tcgcggccgg gcaagcgcag ccgcgccgcg gtgatgattg
    13741 tctgcgcggc gatccttctg agccgcctcg ccgaccattg gtggctggtc ctgccggaat
    13801 tttctcccgg cggcccgttc tggctcgatg tcgccgcgat tttcacgctt ggcggcttga
    13861 tgcttctctt gttcttcgcg ggtctccggt cgcaaggctt agcggtttcc gcggcgactt
    13921 tggcgcgcga tcatggctga gagcgaaccg cgcgtcgcgg ccaatcccaa cgccgcctac
    13981 gagacctccg actggccgtt gcgcccggtc gcctggacat acgccagcgg cctcatcctg
    14041 ctggcgatcg ctgtcggggc gatgctctgg gcctatcctc acacgctagg cgacgtctct
    14101 cgcaagcgcg gggtcgagcc gccggcgccg cggcttcaga tcgatccggc cgaggatttg
    14161 gcggcgttcc gcgccgacaa ggaaaggaag ctgaacagct actattggat cgataaaaag
    14221 aacggcgtcg tccatatccc gatcgagcag gcgatggaga agatcgtcaa gagcgggatc
    14281 gacggattcc cgaaggcgca gccatgatgc gcgcgttctc agtgcccatt ctcgcgacga
    14341 tgcttgcttt tggggcggcc atccatccgg ccgccgccga cgatttctcc gatctcgcgt
    14401 tcgatcctcg ccccggcgcg caattgccgc tgaaggaagt tttcatcgac gaacagggga
    14461 gaatgaccgc gcttggcaat tacttcacgg gcaagccggt cgtgcttgtt ctggattatc
    14521 tgcggtgcaa ggcgctgtgc ggcgtcacgc tcgaacatct gacggccgcg ctgggcgcgc
    14581 tgccgctcga actgggtcgc gactaccaac tcgtcgggat cagcatcgat ccgcgcgaca
    14641 agccgatcga cgccgccgcc gccaaggcga aatatctcgc cctttacggc cacgccggcg
    14701 gcgaatcggg cgcgcatttc ctgaccggcg atggggcggg ggcgcgccgc gtggcggcgg
    14761 cgatcggctt ccgctatcgg tacgacgccg cgctcgatca atacatccat ccggcgggtt
    14821 tcgtcatcgc cacgaccgac ggtcggatca gccgctacat gctgaccgtc ggcccggcgc
    14881 tggccacgct tcaggcggcg ctcggcgacg ccgccgcggc gcgcgtgacg gacccgctga
    14941 cgcggtttct tctcctgtgc catgtcgaag gcgcgccgct cggccgctat gacgggccga
    15001 ttctcgtcgc gttcagcctc gccaacctag cggcgatggc cgccttgatc gtccttttcg
    15061 gcacgatccg gcgccggcgc aacggttgat cgcgatgcgc cactggctcc ccttctggcc
    15121 gttgatcagc gccaagcagg gcgtcgtggt caacaatctc gtcatcgcgg aagtcgccgt
    15181 ctcggcgctc accgtcctcg cggtcttcct catgatggtc accttttgca tccgctaccg
    15241 gcggggcagc caggcttcgc gcgcccattt gcgggaaaag acctggcgga tcgagatcgg
    15301 ctggacgacc gcgaccttga tcgggtttct cgcgctcttc gtctggggcg ccggaatcta
    15361 tgtctggctc tttcaatcgc cgccgggcga catcgaaatc tatgtcgtcg gcaagcaatg
    15421 gatgtggaag ttccagcatc ccggcggaca gcgcgagatc gatgcgctgc atgttccggc
    15481 tggcaagacg atccggctgg tcttttcctc gcaagacgtg attcacagct tctacattcc
    15541 ggccttccgc atcaagcacg acgtcgtgcc tggaacgtac gaaacgctct ggttcaacac
    15601 gacgaagacc ggcgtttacc ggctggaatg cacccaatat tgcggcgacc agcacgccac
    15661 catgaccggc gaagttatcg tcatggagcc gacggcctat gaaaaatggc tgaccgacca
    15721 gggcgtccat cagtctctgg ccgagcaggg cgaagccttg ttccggcagt atggctgcag
    15781 cggttgccac ggcgcgaaca gcaccgtcca cgccccgtct ctggcgggcg tttacggcag
    15841 catggttcaa cttcaggacg ggtcggtgcg gcgcgccgac gaagcctatt tgcgcgattg
    15901 catcctcacg ccgcgcacct tcaccgtcgc cggctatccg ccgatcatgc cgaatttctc
    15961 cggccagatc ggcgaggacg atttgatgaa gctgatcgcc tatatccaat cgcttgctca
    16021 aggagcgcgc catggcgatg atcgccgctg agatcgagcc gacctatctc gacaacgaac
    16081 atacgatcgc ctcgtggctc ctcaccaagg accataagcg cattgcgatg atgttcctgg
    16141 tctcgatcac cgtttttttc ttcatcggcg ggatcgccgc cacgctcatc cgcctgcatt
    16201 tggcgacgcc gcaaggcctg ctgcagccct cgctctacaa ccgcatgttc acgatgcacg
    16261 gcgtgatcat ggtttggttc ttcctcatcc cctcgatacc gaccacgctc ggcaatttcc
    16321 tggtgccgat gatgatcggc gcgcacgacc tcgcctttcc gcggatcaat ctcgccagtt
    16381 ggtacgtctt caacctgtcc gggctcgttc tgctttatgc gctgatcatg ggcggggtcg
    16441 acaccggctg gactttctac acgccctatt cgacggtcta ttcaaacggc tatgttttcg
    16501 ccgcggcgct ggcggttttc gtcaacgggt tttcctcgat catgacggcg ctcaacttcg
    16561 tcgtcaccat tcaccggttg cgggcccccg gcatgacctg ggcgcgcctg ccgctcttca
    16621 tctggggcat ctatgcggtg agcgtcgtca tgttgctggc gacgccggtc ctcgccatgg
    16681 acacgatgct cctcggcgtc gagcggttca tcggcctcgg catgttcgat ccgcggcgcg
    16741 gcggcgaccc gattctttgg cagcacatgt tctggttcta ttcgcacccg gcggtctaca
    16801 tcatgatcct gccgggaatg ggcctggtca gcgagctcat cgcctgttac gcgcgccggc
    16861 cgatcttcgg ctatgcggga atgacgatcg cgatgatggc gattgcgatc ctcagcttct
    16921 tcgtctgggc gcatcatatg ttcgtcgccg gaatttcgat ctattcggcg atcgtctttt
    16981 cgctgaccac cttcttcgtc gcggtgccct cggcgatcaa ggtgttcaac tggaccgtca
    17041 gcctgcacaa gggggcgatc atcttcgacg cgccgatgct ttacgcgctt ggcttcgttc
    17101 ttctcttcac catcggcgga gtcacggggc tcatgctggc gacgctcggc gtcgacgtgg
    17161 cgacgcatgc aacctatttc gtcgtcgcgc atttccacta catcatggtc ggcgcgacgg
    17221 tcagcgcctt tttcggcggc gtccatttct ggtggccgaa aatgaccggc cgtctctatc
    17281 cggaaatgtg ggcgcgtttc gccgccatcc tcaccttcat cggcttcaac gtgacttttt
    17341 tcccgcaatt cattctcggc aatctcggca tgccgcggcg ctactacacc tatccgccgg
    17401 aatggcaatc gtggaacctt ctatcgtcgg ccggcgcctc gatcctggcg gtcgcctatc
    17461 tgctgccctt gttctatctc ggttggtcgc tcgtttacgg caagcgggcc ggccccaatc
    17521 cctggcgcgc caccggactg gaatggctga cgccctcgcc gccccccaag gataatttcg
    17581 agcgcacccc ggtcgtcaca atcggacctt attcctacga tccggaaacc gaccactata
    17641 tcacgcagga cggcgtctcc catgcctagc gcgacgctcc ggcccgagac gcaatatgcg
    17701 acgctcgccc agcagggcga ggtcgcgcaa ctcggcatgt gggtgttcct ggcgaccgag
    17761 acgctgttct tcggcgcgct gatcttcaca tattatctgt accggctttc ctatcccgaa
    17821 gcgatcgccg aggcggcgaa ggacgccgtc ctctggtgcg gcagcgtcaa tatcggcctg
    17881 ctgctgacca gcagcttgac catggtcctg gcgatcaacg cggcggcgca gggtcgccgc
    17941 ggcgccgcga ccggttggct gctgacgacg atcggccttg gcctctgctt cctcggcgtc
    18001 aaaggctacg aatatgtcgc cgactattcg gaccatgtcg tgccggcggt gaatttcgcg
    18061 ctcaagccgg gcgagtcgcc ggtgagcgaa ttgttctggg tctattattt cgtcgccacc
    18121 ggcctccacg cgattcacgt cacgaccggg ctcggcctgt tgatctatat cctgtggcgc
    18181 ctccggcgcg gccggatcag cgccgcctac tacgcgccga tcgaagtcgt cgggctctat
    18241 tggagcttcg tcgacacggt ctgggtgttt ctctatccga ccatctacct tgcaggccgc
    18301 acatgaacat cgaagccaaa gaagcgagct cgtttccctt cgtctgcgcc tggctgcttc
    18361 tgttgatcct ggtcggctta agcgtgctca gcgcttttca aggcctcgcg ctttgggcgg
    18421 cggtgatcga gttcggcatc gccgcgttcc aggccctggt catctttctg ctcttcatgc
    18481 ggctcaaagg cccgccttcg ctcaaatggg tcttcgccgg cacgggcttt ttctggctga
    18541 tctttctcta cgggctcagc atgaccgatt acgcgacgcg aaccggctgg ccgacgaagt
    18601 aaggcgagac tcggcttaaa attcaaacgc cgcctgaacc ggccgcggcg gctccagcct
    18661 tacgccttcg agctccagca tgcgcctttt cgtcgccgcg ccgcccgggg ccgagaagcc
    18721 gccgattttg ccgccggcgg caagaacgcg atggcaaggg atgatcaagg cgaccggatt
    18781 cttcgccatg gcgacgccta cgtcgcgggc ggcttccggc cccgcgccga gcgccttggc
    18841 gagcgcgcca taggtcgtcg tgcggcccca gccgacctgt cgcgccgctg cgtagatgcg
    18901 ctggaagaag tcgtcctggt cggcgaggtc gaggtcgaag cgcgagaaat ccgtctcttc
    18961 gccctcgaaa tagcttttca cggcggcggc ggcttccaga atctctggcg gcggcgcggc
    19021 gggctctgcg ccgggcgtcc gccgccggag caggcgctcg gtcgcttccg cgctgcgcgt
    19081 cggcaattgg aagcgggtga tcccggattg gctccaagcg aggccgcaga agccggcggc
    19141 tgtctcgaag acgaaatagc gttgcgcagc ttggctcatc ggatggcgct cgaaaccggt
    19201 cgcctcagat tgtggagccg cggcggaaat tcaatccgat tcttcgagcc gaaattcggc
    19261 gcgcgcccga taggctcaaa tgaaaacggg cgccgatcgg cgcccgttgc aagcttgtgg
    19321 ggcgaatccc gctcaggcct tcgcctcgtc cttcttcccg gccggccctt cgctgcgctc
    19381 ggcgatgcgc gccgacttgc cgcgccggtc ccgcagataa tagagcttgg cgcggcggac
    19441 cttgccgcgg cgcaggacct tcaccgaatc gatcatcggc gaatagatgg ggaagacgcg
    19501 ctcgacgcct tcgccatagg aaatcttgcg cacggtgaag ctctcgttga ggccgccgcc
    19561 gctgcgcgcg atgcaaacgc cctcataggc ctgaacgcgg gtgcgctcgc cttccttgac
    19621 cttcacattg acgacgacgg tgtcgccggg ctggaactcc ggcactttgc tcgtctgggc
    19681 gagcttggcg gcctgttcgg cctccagcgt ctcgatgata ttcatgataa actcctgccg
    19741 cgacgtcgaa gcttccggca ataagctatt cattctaatg ctttttcagc gaagcttcga
    19801 acgagcgctt cggcgcgctg ttttcatttg aagcgcggct catacaccaa gggagcgacg
    19861 ctgtcgaggc gatgcaaaaa tccatctcgc ggaacgtccg atcgccaccg tcgattcggt
    19921 cggcgccggg cgaaaatgac tgaaaattga cctcttcgcc ggcacccgcg tcttgccaca
    19981 atgttgcttg acgctatttt ccgttcgccg tccggctgaa attcatcacc caatcccgat
    20041 caaggagcga tgcgtgatca cctctaagcc cgcgccggga ccgactcggc gccgcgccat
    20101 cgccgccttg gccgttgcgg ccggcctcgc cgccgcgtgg attgcgccgg ccgcggcgca
    20161 agccacgatc ccgatggatc agttgatggc gcccgggcct ttgccagaca tcgtccaggg
    20221 caaggcggac gcgccggtca cgatcatcga atacgcctcg atgacctgca cgcattgcgc
    20281 cgcctttcac gccgaaacct ggccggcgct gaaggcgaaa tatgtcgata ccggcaaggt
    20341 caagttcatc ctgcgcgaat tcccgctcga cccgctcgcc accgccgctt tcatgctggc
    20401 gcgctgcgcc gggccggaca agcgcaacgc catcgtcgat cttctcttcg acaagcagaa
    20461 agactgggcc ttcgtcgaca agccgctcga agcgctcgcc aacaccgtca agcaggccgg
    20521 catctcgcag ggcgatttcg aatcctgcct gaagaaccag cagctctacg aacaagtcaa
    20581 tcagacgcgc gacaatgcgg cgaagaactt ccacgtcgac gcgacgccga ctttcttcat
    20641 caacggcaag aagctcagcg gcgaaagacc gctcgccgat ttcgacaaga tcatccagcc
    20701 attgctgaaa tgacttgaaa ccgggttgtg cgtttcgcac agctttgccg ccgcgcggcc
    20761 ttgattcggc gcccgccttc atggcccggt aacatgatcg ctcgccgatg attcgggcgg
    20821 cgcgacgatt ttgaccagca tgggttttaa gttgatgccg gcaatccgtc tttgcgagtc
    20881 cgtggcgagc gcgccgaccg agcctgaccg accggctttc ccggtcgttc ccgcgcaagc
    20941 gggaatccag ggagccgcca tgcgcgaaaa atcgacagtc ctggattcct gcttgcgcgg
    21001 gaatgaccat ggcgcctcgc tcgcaacggc gacgccgcga ggtcgggcat gaaattcgac
    21061 aagcttcgcc tcgccggatt caaatccttc tgcgagacga ccgatttcaa gatcgagtcc
    21121 ggcctgaccg ggatcgtcgg gccgaatggc tgcggcaagt ccaatctcgt tgaagccctg
    21181 cgttgggtga tgggcgagaa ttcctacaag aacatgcgcg cctccggcat ggaggacgtg
    21241 atcttttccg gcagcggcaa tcggccggcg cgcaacatcg ccgaggtcgg cctgacgctc
    21301 gacaataccg accgccaggc gccggcggcc ttcaacgacg ccgacatcat cgaggtgacg
    21361 cggcggatcg agcgcgaggc gggctcgacc tatcgggtca atggccgcga agtgcgcgcg
    21421 cgcgacgtgc aattgctgtt cgccgacgcc tcgactgggg cgcgctcgcc ggctttggtg
    21481 cggcagggcc agatcggcga gatcatcgcc gccaagccgc aggcgcggcg cttgatcctc
    21541 gaagaagcgg ccggcgtctc cggcctgcat tcgcgccgcc acgaggcgga attgcgcctg
    21601 aaaggcgcgg aggaaaatct cgtccggctc gaagacgtgt tggcgcagat cgatcagcag
    21661 atcgagagcc tgaagcgcca agcgcggcag gcgacgcgct ataagggcct cgccgccgaa
    21721 atccgccgcg tgcaggcttt ggccctgcat atttcctggc gcgacgagga gcagcgcttc
    21781 gccgaggcgc agcgccggct cgaagccgat acgcgcgatg tcgccgagcg caccgccgat
    21841 caggccgagg cggcgcgcgt ccaaggaatc gccgccgccg ctttgccgcc attgcgcgac
    21901 gaggaggcgc gggccggcgc ggctttgcat cgcctggtcc tggcccgcga ggcgctcgac
    21961 ggcgaggagc gccgcgccaa ggagcggacc gccgagttgg agcgccgcgt cgcccaattg
    22021 gcgcaggacg cgcagcgcga gacggcgttg atcgacgacg ccgcggctac cctggagcgc
    22081 ttggcgcagg aagacgccgc gttggccgaa cagcaggggg cgagcgaaac cgcccggcag
    22141 agcgccgagg cgcggctggc ggaggccgag cagctcctcg ccgcctgcga agcgcggctc
    22201 aacgaggccc aggccgcgct ttccgatctc aacgcccggc gcaatgcgct ttccgccgcc
    22261 ttggccgaag agacgcgccg cctttcccgg ttcgaggccg aatgcgtttc aatcgcgcag
    22321 gagcgcgaca agctcgtcgc cgaggccggc ggcttgaacg agcaaagcga ggacgctgcc
    22381 gctttggagg cgctcaacgc ccgcctcgcc gccgccgaag cccggaccct ggccgctgaa
    22441 gccgctcaag cagaggcgcg cgaggcggag caacgcagcc gcggcccctt ggccgacgcc
    22501 gagcgcagcg cgcagcgcct tgagaccgag gcgcgtacgc tgatgaagct gctcggctcg
    22561 gattcgggcg gattatggcc gccggtcgtc gagtccctct ctgtcgagaa gggctacgaa
    22621 gccgcgctcg gcgccgcgct gggcgacgat ctcgacgcct cgaccaacac gtccgcgcct
    22681 gcccattgga ccgagctcga cggctcattc gagccggctc cgctgccgcc cggcgtgcaa
    22741 tccctcgcga gcctcgtcaa ggcgcccccc gccttgcagc gccggctgtc gcagatcggc
    22801 gtcgtcttgc gcggcgaagg ccgcgccttg ctgccgctgc tgaagcaagg ccagcggctc
    22861 gtgtccaagg agggcgacct ctggcgctgg gacggtttcg tctccgcggc cgaggcgccg
    22921 acgccggcgg cgcggcggct cgccgagaag aaccggctcg gcgatttgac ccgcgacgcc
    22981 gaggcggccc gacaagccgc cgaggcgctc aaggcgcaag cggtcgccgc ccaggcggcg
    23041 ctccgcgaag ccgcttcggc cgagcaagcc gcccgtcatg aggcgcgcga agcgcgccgc
    23101 ctggtcgacg cagcgcgcga gcgcctcgcc gccttggagc gccgccgcgc ccagacggcc
    23161 gcgcgcctct ccgccttgga ggaggccgcc tcgcgcgcca gcgcctcgcg cgacgaagcc
    23221 gcgatgcgaa aaagcgaggc cgaggcggcc ctggccggcc ttgtcgcgac cgaccacctt
    23281 gctctggatt tggagcgcgc ccgcggcgaa gcgaccaagg ccagggcgga atgcgccgag
    23341 gcccgcgccg cctcgcagaa cctcgcccgt gaagcggcgg cgcgcgctgc gcggcggcaa
    23401 gccatcgccg aggaccagaa atcctggagc caacgccgcg agcgcgcgga agtccagatc
    23461 gccgagttcg ccgcccgcca atccgaggcg acgcgcgaat tggagcctct cgccgacgcg
    23521 ccggccgcct tcctgcagcg ccgccgcgcc ctgatcagcg agatcgacgc ggccgagcag
    23581 gcgcgcaaga aagcgagcga cgcgcgcgtc gcggccgaga cggcgctgag cgaggcggat
    23641 cgcgccgcca aggcggcgtt ggaggcgatg agcgcggcgc gcgaagctcg cgccgcctcc
    23701 gaggagcggc tcgaagccgc gcgccagcgc cgcgaaaacc tgctgcatac gatcgccgtc
    23761 gagctcgaga ccgagccgca cgccctcgcc gggcttgccg gcgtcaaggc cgacgaggac
    23821 ttgcgggagc tggcgcaagt cgagcgcaag ctcgaaaacc tcaagcagga gcgcgagcgg
    23881 ctcggcgcgg tcaatctgcg cgccgaagac gagctcgtcg aggtcgaggc gagccgcagc
    23941 cgcctcatcg ccgagcgcga cgacctgatc gaggcgatca agcgcctgcg ggtcgcgatc
    24001 ggcagcctca acaaggaggg acgcgagcgg ctcgtcgcgg cgttcgacgt ggtcaacaag
    24061 cacttcggcg aactgttcgc gaccttgttc gagggcggct cggcctatct gcagctcatc
    24121 gaatcggagg atccgctcga agccgggctc gaactgatcg ccaagccgcc gggcaagaag
    24181 ccgcagacgc tgaccctgct ttccggcggc gagcaggcct tgacggcgat gtcgctgatc
    24241 ttcgccgtgt tcctgaccaa tccgtcgccg atctgcgtgc tggacgaagt cgacgcgccg
    24301 ctcgacgatt ccaatgtcga gcgcttctgc gatctgctcg aggagatgcg caagcgctcc
    24361 gacacgcgct tcgtgacgat cacgcataat ccgatcacga tggcgcggat ggatcgcctg
    24421 ttcggcgtga ccatggccga acgcggcgtc agccaactcg tctcggtcga tctcgccgag
    24481 gcggagcggt tcctcgaggc gagctgagca cggggctagg cgagcccctt ttcgatccta
    24541 gcgatctcgg ccgaagcgct cgcccgggtc ttgcgctcgg cctcgcggta aagcgcgacg
    24601 agccgctcgc cggcttcggt cagcgccgcg ccgccgcctt tggcgccccc gggccgcgcc
    24661 gtcaccagcg gttcggcgaa catcgtgttg agctcctcga ccagcagcca ggcgcggcga
    24721 taggacattt cgtgtgcgcg gccggcggcg gaaatcgagc cggtgtcgcg gatcgcttcg
    24781 agcagcgcga ccttgccggg accgagtctt cggccggcgt cgaaatcgag gcgaagcgtg
    24841 acgcgcgtcg acatcgggtc attgtgcaat aatggaggcc gccgaggcaa acctctcctg
    24901 ctccccgctt tgcggctacg gcatgcacac atcttgaagc gcggcgcgcc tgagccgtgc
    24961 tcgccgtcaa ggccaagcgc gattcgcgcg tcgctcgcca aagcaggctc aggagaaaat
    25021 cagttggccc cgcgccgaga tgcgtgcatg ccgtagcgcc ttgcacggag aagggagttc
    25081 cctgcgcaat ggcgggagcg atcgaatgag cgaaagcgac atcctgatcg agacgtgcgc
    25141 gccctccgat ttggacgatt gggtcgccct tcgcgccgcg ctatggccgg acgagtcggt
    25201 cgaggcgctt cgcgcccatg ccgaggagct gatgcgcaaa ggcggcgacg ccgcgacttt
    25261 cctcgcccgc g
//
LOCUS       DATGJW010000019         5252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1350747, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000019 DATGJW010000000
VERSION     DATGJW010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5252
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350747"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..663
                     /locus_tag="VKU03_00630"
     CDS             <1..663
                     /locus_tag="VKU03_00630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HLJ69790.1"
                     /translation="QAAIEAGLVRINGEILRKASTLIETDARIEAEAPHPWVSRGGVK
                     LAAALDAFKLDPTGLVCLDVGASTGGFTDVLLSRRARLVYAVDVGRDQIDPRLRADPR
                     VVVHESLDARKIVREMFEAPPQAIVCDVSFISLRHVLPHVLPLAADGAWLAALIKPQF
                     EAGRDKVKKGTVKDVATQSEVCAAIIASVAALGWTSLGVVPSPIRGGDGAEEFLLGAR
                     RG"
     gene            656..1924
                     /locus_tag="VKU03_00635"
     CDS             656..1924
                     /locus_tag="VKU03_00635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLJ69791.1"
                     /translation="MAEAVEELNIASLGSRGEGIVKSAQGAIYIPYALPGERVRARVA
                     GDRGTLLDILTPSHKRAAPLCPYFGDCGGCAAQHMNPVLYGQWKRDILLRALAQAHVE
                     TDVHPLVDAHGEGRRRATFHARYTSEGTSVGYMQARAHRVVAIETCPILAPSMSAALA
                     SAREIARALHPLGKPLDIAVTATLAGLDIDFRGAGALDFTLRQSLIAVAGRLDLARLS
                     SHGEILIERRAPEVLMGAARVTPPPGGFLQATAEGERVLAALAIEAVEGERVADLFCG
                     AGAFALPLAERFKVHAVELDAGALAALTRAARDTRGLLPMSQEPRDLFRRPLTPEELK
                     RFDTVLFDPPRAGAAAQAAEIAKSGAPRVIAISCNPATFARDARTLIDGGYRLERATP
                     IDQFRFSPHLEIVGVFTRPAEKKRPRGLLG"
     gene            1921..3339
                     /locus_tag="VKU03_00640"
     CDS             1921..3339
                     /locus_tag="VKU03_00640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010658346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLJ69792.1"
                     /translation="MNDAAADKRLSELAAIVGQAHVVNDPQLLASRLVEPRGLYHGRA
                     LALVRPGATEEVARLLAYCNEKRLPIVPQGGNTGLVGGQIPDESGAAIILSLQRMNKV
                     REVDAASNSMIVEAGVTLAAAQAAAENADRLFPLSLASEGSCTIGGNLSTNAGGVGVI
                     AYGNTRDLALGLEVVLANGSVLSGLNKLRKDNTGYDLKNLFIGAEGTLGVITAASLKL
                     FPRPRAMATAFVGLPDPAAALKLLALARQYVGGGVTSFELLPRIGIEFVLRHAPGSRD
                     PLGERHPWYVLIELSSQIAARLDETLAELLQAAMEQEIILDGAVAATLEQRKAFWRLR
                     EDMSDVQRAEGGSIKHDVSVPMGTAADFIAEATASVLALMPEARVVAFGHLGDGNIHF
                     NVSQPIGADKAAFLARWEEVNSVVHAVVARYKGSISAEHGIGQLKRALLRQTKDPVAL
                     DVMRRLKATLDPNGVLNPGKVL"
     gene            3525..4193
                     /locus_tag="VKU03_00645"
     CDS             3525..4193
                     /locus_tag="VKU03_00645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HLJ69793.1"
                     /translation="MSFDAANYIICSFDARKDDIKLFWKGTDDKAYGGFSRLATALSA
                     HGRALRFAMNAGMFEEDLSPVGLYVEDGRLRHRADLREGASNFHLKPNGVFYIGDSGA
                     GVVETSRFLATGLKTRFATQSGPMLVLDGRIHPKISPTGASAKIRNGVGVREGSQVVF
                     AISEEPVTFHAFAQLFRDRLNCPNALYFDGSVSSLYAPNLNRRDDPARPIGPIVGVIA
                     PTGG"
     gene            complement(4222..5007)
                     /locus_tag="VKU03_00650"
     CDS             complement(4222..5007)
                     /locus_tag="VKU03_00650"
                     /EC_number="1.1.1.194"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HLJ69794.1"
                     /translation="MLFGKTIVVTGVSSGIGARTAELAATLGADVIGVDIKEPSEPLS
                     AFIKTDLSSPQSIAELADTLPKRIDALCNVAGLSGLVGAAKTLAVNFYGLRAVSEALA
                     PRLREGGTVVNVASIAGYGWRANLERAKAMIAPPGFPDIAALLAAHKVPDAEGYPLSK
                     ELLLLWTMRAAHQPLFKDRGIRVNAVSPGPVVTPILREFRSVFGDPRVDSDIARVGRA
                     GAPPDIAPVILFLCSDGARWINGANVPADGGLEASVNAEALGF"
BASE COUNT          916 a         1736 c         1628 g          972 t
ORIGIN      
        1 caagcggcga ttgaggccgg cttggtacgg atcaatggcg aaatcttgcg caaggcctcg
       61 acgctcattg agacggacgc gcgaatcgaa gccgaggcgc cgcatccctg ggtctcgcgc
      121 ggcggcgtca aacttgcggc tgcgttggac gcgttcaaac tcgatccaac cggcctcgtt
      181 tgcctcgatg tcggcgcatc gacgggcggc ttcaccgacg tgctcctctc ccggcgcgcg
      241 cgtctcgtct acgccgtcga cgtcggccgg gatcagatcg atcctcgctt gcgcgccgat
      301 cctcgcgttg tcgttcatga atctctcgat gcgcggaaga tcgtaagaga aatgttcgag
      361 gccccgccgc aggcgatcgt ctgcgacgtc agcttcattt cgttgcggca tgtcctgccg
      421 catgttcttc cgctcgccgc tgacggcgct tggctggccg cgctgatcaa accgcaattc
      481 gaagccggtc gcgacaaagt gaaaaagggc accgtcaagg acgtggcgac gcagagcgag
      541 gtctgtgccg cgatcatcgc cagcgtcgca gcgcttggct ggacgagtct cggcgtcgtt
      601 ccttcgccga tccgcggcgg cgacggcgcc gaggaattcc ttctcggcgc gcgccgtggc
      661 tgaggccgtc gaagaattga atatcgcctc gctggggtcg cgcggcgaag gcattgtgaa
      721 aagcgcccaa ggcgcgatct acatccccta cgcattgcca ggcgaaagag tacgcgcgcg
      781 cgtcgccggc gatcgcggaa cgctcctgga tatcttgacg ccgagccata agcgcgccgc
      841 gccgctttgt ccctattttg gcgattgcgg cggttgcgcc gctcaacaca tgaatccggt
      901 tctctacggg caatggaaac gcgacattct tttgcgcgcg cttgcgcaag cgcatgtcga
      961 aacagatgtg catccgctcg tcgacgctca tggcgagggg cgacggcgcg cgacttttca
     1021 cgcccgctac acaagcgaag gaacaagcgt cggctatatg caagcgcgcg cccatcgcgt
     1081 cgtcgctatt gaaacctgtc caatcctcgc gccgtcgatg agcgccgccc tcgcaagcgc
     1141 gcgtgaaatc gccagagctt tgcatccgct cggcaagccg ctcgatatcg ccgtcaccgc
     1201 cacccttgcc ggcctcgaca ttgattttcg cggcgcgggt gctttggatt tcacgctgcg
     1261 gcaatcgctt atcgccgtcg ctgggcggct cgatcttgcc cggctctcca gccatggcga
     1321 aatcctcatc gagcgccgcg cgccggaagt cctgatgggc gcggcgcgcg tgacgccgcc
     1381 gccaggcggt tttctccagg cgaccgccga aggcgaacgt gttctcgccg ctttggcgat
     1441 cgaggcggtg gaaggcgaac gcgtcgccga tctcttttgc ggcgcgggcg ctttcgcgct
     1501 gcctctcgct gagcgcttca aggtccatgc ggttgaactg gatgcgggcg cgctcgccgc
     1561 tctcacccgc gcggcccgag atacgcgagg tctattgccc atgagccaag agccgcgcga
     1621 tctcttccgc cggccgttga cgccagaaga attgaagcgc ttcgatacgg tgcttttcga
     1681 cccgccgcgc gccggtgcgg cggcgcaggc cgcggaaatc gctaagagcg gcgcgccgcg
     1741 ggtcatcgcc atctcctgca atcctgcgac tttcgcgcgc gatgcacgaa ccctcatcga
     1801 cggtggctat cggctggagc gggcgacgcc gattgaccag tttcgttttt cgccgcattt
     1861 ggaaatcgtc ggcgtgttca ctcggcctgc tgagaaaaaa cgcccgagag gattgcttgg
     1921 atgaacgatg cggcggcgga caagcgccta tcggaactag cggcgatcgt cggccaggcg
     1981 catgtcgtca atgatccgca attgctggcg agccgactcg tcgaaccccg cggcctctac
     2041 cacggccgcg ccttggcgct cgtccggccg ggcgcgacgg aggaagtagc gcgcctcctc
     2101 gcctattgca acgagaagcg tctgccgatc gtcccgcagg gcggcaatac cggcctggtc
     2161 ggcggccaaa ttcccgacga aagcggcgcg gcgatcattc tgtcgctgca gcgcatgaac
     2221 aaagtgcgcg aagtcgatgc ggcctcgaac agcatgatcg tcgaagccgg cgtcacgctc
     2281 gccgccgctc aggctgcggc tgagaacgcg gatcggctgt ttccgctgtc gctcgcttct
     2341 gaagggtcat gcacgatcgg cggcaatctt tcgaccaacg ccggcggcgt cggcgtcatc
     2401 gcctatggca atacgcgcga tcttgctttg gggctggaag tcgttctcgc caatggttcg
     2461 gtcctgtccg gcctcaacaa actgcgcaag gacaataccg gctatgatct gaagaacctt
     2521 ttcatcggcg cggaagggac gctcggcgtc atcacggccg cgtcgctgaa actcttccca
     2581 cgcccgcgcg ccatggcgac ggcctttgtc ggcctgcctg atccggccgc cgcgctcaaa
     2641 ttgctggctc tggcgcgaca atatgtcggc ggcggcgtca cgagtttcga gctcctgccg
     2701 cgtatcggga tcgaattcgt tttgcgccac gcgcccggct cgcgcgatcc gctcggcgaa
     2761 cgccatccct ggtacgtcct gatcgaactc tcctcccaaa tcgcggcgcg tctcgacgag
     2821 actctcgccg aacttttgca ggcggcgatg gagcaggaga tcatcttgga cggcgccgtc
     2881 gccgccacgc tcgaacagcg caaggccttt tggcgcctgc gcgaggacat gtccgacgtg
     2941 cagcgcgccg agggcggatc gatcaagcac gatgtcagcg tgcccatggg aacagcggcc
     3001 gacttcatcg ccgaggcgac agcctctgtc ctcgccttga tgcccgaggc gcgcgtcgtc
     3061 gctttcggcc atctcggcga cggcaatatt catttcaacg tctctcagcc gatcggcgcc
     3121 gacaaggcgg ccttcctcgc ccgctgggag gaagtgaaca gcgtcgtcca tgcggtcgtc
     3181 gcgcgctaca agggctcgat ctccgccgaa catgggatcg gccaattgaa acgcgcgctc
     3241 ttgcgtcaga ccaaggatcc cgtcgccctt gatgtgatgc gaaggctcaa ggcgacgctc
     3301 gacccgaatg gcgtgctcaa tcccggcaag gtgctttgac gattcgtttc aggttatgat
     3361 gcgcgaagat tcgctcacga gccgtcgcgc gcccgcccat gaaccgacag acgcagcaga
     3421 cgacatcgcc cctggcgcgg cgcgtccgcg ttatgatcgc gcttgtcctg gcatggcttt
     3481 tcgcggggcc ggccttcgct caaacggcga acccttgcgc atcgatgagc tttgacgccg
     3541 ccaattatat catctgctcc ttcgacgccc gcaaagacga catcaagctg ttctggaaag
     3601 ggaccgacga caaggcctat ggcggctttt cgcgcctcgc cacggcgctc tcggcgcatg
     3661 gtcgcgcgct gcgtttcgcg atgaacgccg gcatgttcga agaggatctt tcgccggtcg
     3721 gtctttatgt cgaggacggt cgcttgcgcc atcgcgccga tctcagggaa ggcgcgtcca
     3781 attttcacct caagccgaat ggcgttttct atatcggcga cagcggcgcg ggcgttgtcg
     3841 agacatcgcg ttttctcgcc accggtttga agacgcgctt cgcgacgcag tcaggtccga
     3901 tgctggtcct cgatggccgc attcatccca agatttcgcc aaccggcgcc tcggcgaaga
     3961 tacgcaatgg cgtcggcgtc cgcgagggtt cgcaagtcgt cttcgcaata tccgaagaac
     4021 ccgtcacttt tcatgctttc gcgcaattgt tccgcgaccg gctcaattgc ccgaacgcgc
     4081 tctacttcga cggctccgta tcgagcctct atgcgccgaa cttgaaccgt cgcgacgatc
     4141 ccgcgcgacc gatcgggccg atcgtcggcg tgatcgcgcc gacgggcgga tagcgcctga
     4201 catgcggcgc tgaaccttcc ctcagaaccc cagcgcttcc gcattcaccg acgcctcgag
     4261 cccgccatcc gccggcacat tcgcgccatt gatccagcgc gcgccatccg agcagaggaa
     4321 taagatcacc ggcgcaatat ccggcggcgc cccggcgcgg ccgacgcgag caatgtcgct
     4381 atcgacgcgc ggatcgccga agacgctacg aaactccctc aggatcggcg tcaccaccgg
     4441 acctggactg acggcgttga cgcgtatgcc gcgatccttg aacaagggct gatgcgcggc
     4501 gcgcatggtc cagagcagca gcagctcttt ggacagggga tagccttcgg cgtccgggac
     4561 cttatgcgcg gcgagcagcg cggcgatgtc gggaaagccc ggcggcgcga tcatcgcctt
     4621 cgcccgctcg agattggcgc gccagccata gccggcgatg gaggcgacgt tgacgaccgt
     4681 gccgccctcg cgaagccgtg gcgcaagcgc ttcgcttacg gcgcgaagtc cataaaaatt
     4741 caccgccagc gtttttgccg cgccgacgag gccggagagg cccgcgacat tgcaaagcgc
     4801 gtcgatgcgc ttgggcagcg tatcggcaag ttcggcgatt gattgcggcg aagaaagatc
     4861 ggtcttgatg aaagcgctga gcggctccga cggctccttg atgtcgacac cgatcacgtc
     4921 ggcgccaagc gtcgccgcca actccgccgt ccgcgcgccg atgccggacg agacgccggt
     4981 gacgacgatg gttttgccga acagcatgaa gcttcctccc tgatcattca tgcgccgcgt
     5041 cattgcgagc gaggcgaagc aatccaagcg gccgaatgga ttgcttcgtc gcttcgctcc
     5101 tcacaaagac gacgcgtcac ggcttctcaa tcgctgtcaa tcggtaccca accggcaaca
     5161 attgcagcgc aaatcgctcc tgcaacagtc cccaaagtcc cttcggcagg aacagcgaaa
     5221 acaacagcgc cgtcgcgccg agcccgacga gg
//
LOCUS       DATGJW010000020         3973 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1143263, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000020 DATGJW010000000
VERSION     DATGJW010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3973)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3973)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3973
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1143263"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1315
                     /locus_tag="VKU03_00655"
     CDS             <1..1315
                     /locus_tag="VKU03_00655"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HLJ69795.1"
                     /translation="RKKRDGVALSAAEITAFIQGATRGDVSEGQIGAFTMAVYLNGMT
                     IDETAALALAMRDSGRVLDWAGLALDEARLIDKHSSGGVGDEKITLLVVPLAAACGVL
                     VPNLSGRGLDYCAGEIDMLDAIPGYQTAPPPDLFMQTVKDVGGAIISPTLDLAPADRK
                     LFYVRDVTATVESIPLITGSILSKKLASAPRGLVVSVGAGSGAYMASLDDARRLASSM
                     AEVSARAGTPNVMLLTDLDSVLGTAVGNAVGVAETVEFLTGRRRDPRVLDLVLSLTAE
                     MVVLAGIESDLAAARRRAAAMLDSGAAAERFARMVKALGGPGDFLERAASYLPAAPLI
                     RPVFAERPGFVAGMNAKEIGLTLVALGGGRKRPDETIDLGVGLAEFIQVGDFADRDRP
                     LCVVHARDEESYELAARRIRASVQTSAESAGAAGPIVKERLSRRP"
     gene            1514..2581
                     /locus_tag="VKU03_00660"
     CDS             1514..2581
                     /locus_tag="VKU03_00660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017265517.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLJ69796.1"
                     /translation="MRKPLNVERPTGGVSRRSFLQTSFGAATLAGAGALSSANIAAAA
                     ESGLVALVHTQAAGDNGPIDSMISALKKLSGEKKFPIRTIYAQDAATYGTIFKSLADA
                     GAAVIVATFNEVAEPMKALAPKYPKTKWIQIFGDPINPPIPNFETVSYDYYLGCYLSG
                     MFAAKVSKTGKIGYIGGVSLPPLNADVNSLKAAIAAVNPKATFTHAFAGSFQDPAKGH
                     EIASQMYQSGIDYIQTDSAATDTGIIQAANEGQDRMVSAISPAQYKIGPKTVAALVEL
                     NFGQSLYNEVSKALGPDFKGGHLATGLGTGVIDFVLSPTWQEQGPKDLVAKAKSVWPE
                     IEKAKADIISGALKPPFDTKL"
     gene            2712..>3973
                     /locus_tag="VKU03_00665"
     CDS             2712..>3973
                     /locus_tag="VKU03_00665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ69797.1"
                     /translation="MIEAIPSEAPWPSALQCRDISVQYGEVAALVGVSLAFAPKLIHA
                     VVGQNGAGKTTFARVAAGIVKPISGTLAIAGKEISTGNVGQARAAGVELVHQSFALPP
                     SFTVAEAMEFGAERGRPFFSRRGLEARWRKHLENLGVRAGLRDRIRDLPVEAQQGVEI
                     ARALVADAKVLILDEPTAVLSPSGIETLFERLKSLKASGVTIILILHKIREVLGIADT
                     VTVLRGGRLVEGPVAARATNARRLSASIIGALAEQDIAADEAALVGATGETKAAPVPA
                     EVSRAAPLLAMERIVTRADSEGPGLEEASLTIGRGEIVGVAGVEGNGQRTLVRVLANL
                     VDAQSGRILLGGRDITRTPLSERRARGLRVIPFERNSEGLSLLSALWENWSARQLVLG
                     PALKLINPARYRKACDVSLKEWDVRYHTT"
BASE COUNT          702 a         1321 c         1289 g          661 t
ORIGIN      
        1 ccgcaagaag cgcgacggcg tcgcgctgag cgccgccgaa atcaccgcct tcatccaagg
       61 cgcgacgcgg ggcgatgtct ccgagggcca gatcggcgcc ttcacgatgg cggtctatct
      121 caatggcatg acgatcgacg agacggcggc gctggccctc gcgatgcgcg attccggccg
      181 cgtcctcgat tgggccggcc ttgcgctgga cgaggctcgt ctgatcgaca agcattcctc
      241 cggcggcgtc ggcgacgaga aaatcacgtt gctcgtcgtt cctctggcag ccgcctgcgg
      301 cgttctcgtt cccaatcttt ccgggcgcgg tctcgattac tgcgccgggg agatcgacat
      361 gctcgacgcc attccgggct accagacggc gccgccgccg gacctcttca tgcagacagt
      421 gaaggatgtc ggcggcgcga tcattagccc gacgctcgac ctcgctccgg ccgaccgcaa
      481 acttttctat gtgcgcgacg tcacggcgac agtggaaagc atcccgctga tcaccggctc
      541 aatcctgtcc aagaagctcg catccgcgcc gcgcggcctg gtcgtttccg tcggcgccgg
      601 ctccggcgcc tacatggcga gcctcgatga cgcccggcgg ctcgcaagca gcatggctga
      661 ggtctcggcc cgcgccggaa cgcccaatgt catgcttctg acggatcttg attcggtgct
      721 ggggaccgcg gtcggaaacg ccgtcggcgt cgccgagacg gtggagttcc taaccggccg
      781 ccgccgcgac ccgcgcgtcc tcgacctggt tctttccttg accgccgaga tggtcgtctt
      841 ggccggaatc gagtccgacc tcgccgccgc gcgccggcgg gccgccgcca tgctcgatag
      901 cggcgcggcc gccgagcgtt tcgcgcggat ggtcaaagcg ctgggcggac ccggcgactt
      961 cctggagcgg gcggcgagct atcttcccgc agcgcctctc atccgccccg tcttcgcgga
     1021 gcgccccggt ttcgtcgccg gaatgaacgc caaggagatt gggctgaccc tggtcgcgct
     1081 gggcggcggc cgcaagcgac ccgacgagac gatcgacctc ggggtggggc tggcggagtt
     1141 cattcaggtg ggagacttcg ccgatcgcga tcggccgctc tgcgtcgtcc acgcccgcga
     1201 cgaggagagc tatgaactcg ccgcacggcg catccgggcg tccgttcaaa cgagcgccga
     1261 atccgccggc gcggctggac ccatcgtgaa agagcggttg tcgcggcggc cttaggagat
     1321 gcaggccaga cggagcggta aaaagttgac gctttggtca ggatacggca tcgttgtctc
     1381 cagccgccag gaggccctga agaacacatc gcgttgttcg caagaaataa aattcacgcc
     1441 ggttcggcag atggcgattc ggtacgattt tcgtgagttc tgcgcgccaa cgcagcggag
     1501 agggaggatg tcaatgagaa aaccgttgaa tgtcgaacgg ccgaccggcg gcgtatcacg
     1561 ccgctcgttt ctgcaaacct cgttcggcgc ggcgacgctc gccggcgccg gcgctctttc
     1621 ctcggccaat atcgcggcgg cggccgaaag cggattggtc gcgctggtcc atacccaggc
     1681 cgccggcgat aacgggccga tcgactcgat gatcagcgcg ctcaaaaagc tgtcgggcga
     1741 gaagaaattc ccgatccgca ccatctacgc gcaggacgcc gcgacctacg gcacgatttt
     1801 caagagcctc gccgacgcgg gggccgcggt gatcgtcgcg accttcaacg aggtcgccga
     1861 gccgatgaag gcgttggcgc cgaaatatcc caagaccaag tggatccaga ttttcggcga
     1921 tccgatcaac ccgccgattc cgaatttcga gaccgtctcg tacgactatt atctcggctg
     1981 ctatctttcc ggcatgttcg cggcgaaagt ctcgaagacc ggcaagatcg gctatatcgg
     2041 cggcgtcagc ttgccgccgc tcaatgcgga cgtgaattcg ctgaaagccg ccatcgctgc
     2101 ggtcaatccg aaggccacgt tcacgcacgc tttcgccgga tcgtttcagg accccgccaa
     2161 ggggcacgag atcgccagcc agatgtatca aagcggcatc gattatatcc agacagactc
     2221 ggcggcgacc gacacgggga ttatccaagc cgccaatgaa ggccaggacc gcatggtgag
     2281 cgcgatctcg cccgcgcaat acaaaatcgg ccccaagacc gtggcggcgc ttgtcgagct
     2341 gaatttcggc caatcgctct acaacgaagt ctcgaaagct ttggggccgg acttcaaagg
     2401 cggccatctc gccaccggac ttggcacggg cgtgatcgac ttcgtgttgt cgcccacctg
     2461 gcaggagcaa ggcccgaagg acctcgtcgc caaagcgaaa tcggtttggc cggaaatcga
     2521 aaaagccaag gccgacatca tttccggcgc gctcaagccg cctttcgaca ccaagctata
     2581 agcagcccat gcggggtcgg gccggcccaa tattcgccgg tccgacggcc gccgccgcgc
     2641 tggacggaaa tcgccgcctt gactcctatt ctggtcgctc gatgaatagc ggcggacagc
     2701 gcgaggcggc cgtgattgaa gcgatcccca gcgaggcgcc ttggccatcc gcgctgcaat
     2761 gccgcgacat ttcggttcaa tacggcgaag tcgccgcttt ggtcggggtg agcctcgcct
     2821 ttgcgccgaa actgatccat gcggtggtcg gacaaaacgg cgcgggcaag acgaccttcg
     2881 cccgcgtcgc ggccggcatc gtcaagccga tctcgggaac gctggcgatc gccggcaagg
     2941 aaatctccac cggcaatgtc ggccaggcgc gcgccgccgg cgtcgaattg gtccatcaaa
     3001 gtttcgccct gccgccctcc ttcaccgtcg ccgaagcgat ggagttcggc gcggagcgcg
     3061 gccggccgtt cttttcccgc cgcggcctcg aggcgcgctg gaggaagcac cttgaaaatc
     3121 tcggcgtgcg cgccggcttg cgcgaccgca tccgcgattt gccggtcgag gctcagcaag
     3181 gcgtcgagat cgcgcgcgcc ctggtcgccg acgccaaggt gctgatcctc gacgagccga
     3241 ccgcggttct ctcgccgagc gggatcgaga cgctgttcga gcgcctcaag agcctgaagg
     3301 cgagcggcgt caccatcatc ctcatcctcc ataagatccg cgaagtgctc ggcatcgccg
     3361 acaccgtcac ggtcttgcgc ggcggcaggc tggtcgaggg cccggtcgcc gcccgcgcca
     3421 ccaacgcgcg gcggctgtcg gcctcgatca tcggcgcgct ggccgaacaa gacatcgccg
     3481 cagacgaagc tgcgcttgtc ggcgcgaccg gcgaaaccaa agccgcgcca gtcccggccg
     3541 aggtcagccg cgcggcgccg cttctcgcca tggagcgcat cgtcacgcgc gcggattcgg
     3601 aaggacccgg cctggaagag gcgagcctca cgatcgggcg gggcgaaatc gtcggcgtgg
     3661 ccggggtcga aggcaacggc cagcgcactc tggtgcgcgt cctggccaat cttgtcgacg
     3721 cgcaatccgg ccgcatcctg ctcggcggcc gggacatcac aaggacgccg ctcagcgaaa
     3781 ggcgcgcgcg cggcttgcgg gtcattccgt tcgagcgcaa cagcgaaggc ctgagcctct
     3841 tgagcgcgtt gtgggaaaat tggtcggcgc gccagctcgt gctcggcccg gcgctcaagc
     3901 tgatcaatcc ggcgcgctat cgcaaggctt gcgacgtctc gttgaaagaa tgggacgttc
     3961 gctatcatac gac
//
LOCUS       DATGJW010000021         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_209606, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000021 DATGJW010000000
VERSION     DATGJW010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_209606"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(160..474)
                     /locus_tag="VKU03_00670"
     CDS             complement(160..474)
                     /locus_tag="VKU03_00670"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLJ69798.1"
                     /translation="MKPFVYRLLPPRPSFAQDMTEEDRAVMLEHSAYLRRLAAKGEAV
                     AFGPVLDPKGSWGVGLFQVESAERMEEIARADPAIRSGRGFAYEILPMAALVTGAGAH
                     PN"
     gene            575..901
                     /locus_tag="VKU03_00675"
     CDS             575..901
                     /locus_tag="VKU03_00675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septum formation initiator family protein"
                     /protein_id="HLJ69799.1"
                     /translation="MVVKTRVRAILLPIAFYLVSGAASGFFVWQASIGDRGLHAKAHY
                     QAQMAALSAQLTSLQEEHKALQHKVGLMRDNAIDRDLLDEEARAKLDRVDKNDLVVFT
                     NPVKLR"
     gene            1198..2508
                     /gene="glyA"
                     /locus_tag="VKU03_00680"
     CDS             1198..2508
                     /gene="glyA"
                     /locus_tag="VKU03_00680"
                     /EC_number="2.1.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004372 - glycine hydroxymethyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0070905 - serine binding [Evidence IEA];
                     GO_process: GO:0019264 - glycine biosynthetic process from
                     serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydroxymethyltransferase"
                     /protein_id="HLJ69800.1"
                     /translation="MTIAVATPADGRIRSNSFFSAPLAEADPEIAKAVELELGRQRDE
                     IELIASENIVSRAVLEAQGSVMTNEYAEGYPGRRYYGGCQFVDIAEKLAIERACKLFS
                     CKFANVQPNSGSQANQGVFMALMQPGDTFMGLDLAAGGHLTHGSPVNMSGRWFKPVSY
                     TVDRDSHRIDMEGVERLAHQHKPKLIIAGGSAYARHWDFKRFREICDAVGAYFVVDMA
                     HFAGLVAGGAHPSPFPHAHVATSTTHKTLRGPRGGLILTDDEDLAKKINSAIFPGLQG
                     GPLMHVIAAKAVAFGEALTQEFKNYAQAVVVNAKALAAPILAGGYALVTGGTDNHLML
                     VDLRPKRLTGKAAEAALGRAHITCNKNGVPFDTEKPTITSGIRLGSPAATSRGFGAAE
                     FARVGELIVETLDGLASAGESGNAATEARVRASVAELTRRFPIY"
     gene            2548..3054
                     /gene="nrdR"
                     /locus_tag="VKU03_00685"
     CDS             2548..3054
                     /gene="nrdR"
                     /locus_tag="VKU03_00685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003616064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140110 - transcription regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator NrdR"
                     /protein_id="HLJ69801.1"
                     /translation="MRCPYCGSLETQVKDSRPTEDSSSIRRRRVCPDCGGRFTTFERV
                     QLRELTVVKKSGRRAPFDRDKLQRSVEVALRKRPVASDRIERLVNGIVRQLESQGDGD
                     IPTDRIGELVMEGLKGLDDVAYVRFASVYRNFREARDFNAIVGELSAEPERPAKPAGN
                     GRKAPAES"
     gene            3026..>3759
                     /gene="ribD"
                     /locus_tag="VKU03_00690"
     CDS             3026..>3759
                     /gene="ribD"
                     /locus_tag="VKU03_00690"
                     /EC_number="1.1.1.193"
                     /EC_number="3.5.4.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA];
                     GO_function: GO:0008835 -
                     diaminohydroxyphosphoribosylaminopyrimidine deaminase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     diaminohydroxyphosphoribosylaminopyrimidine
                     deaminase/5-amino-6-(5-phosphoribosylamino)uracil
                     reductase RibD"
                     /protein_id="HLJ69802.1"
                     /translation="MGARRPPSLKVNDRAALDERFMAAALALGRRGLGRAAPNPAVGA
                     LIVRDGVVVGRGFTAAGGRPHAERSALDQAGHLAKGATIYVTLEPCAHQGRGPPCAAA
                     IVEAGIACVVSAMEDPDPRTAGQGHALLRRAGIEVAVGVGAAAARRDHLGHILRVSQN
                     RPAVTLKLAQTADGFAAGARYDPRLAITGEAANSRVQILRAMHDAIMIGIGTALGDDP
                     LLTVRFPGAEARPLRVVLDPRLSLPPR"
BASE COUNT          609 a         1307 c         1210 g          633 t
ORIGIN      
        1 cccagaggat cgccctcacc ctagccgtcc ccgcaaagcg ctaaagggga cgcgcggtgt
       61 ttgtagccgc ggtcgtcgac cgcggctaca gtccctctcc cgcttgcggg agaggtgaac
      121 cgcagcgctt aaagccgagc cgtcggaagc gctccgtcgt cagttcggat gcgctcccgc
      181 gccggtcacc agcgcggcca tgggcaagat ctcataggcg aagccgcgcc cgcttcggat
      241 cgccggatcg gcccgcgcga tctcctccat ccgctcggcg ctctcgacct ggaaaaggcc
      301 gacgccccac gagcctttcg ggtcgaggac cggaccgaag gcgaccgcct cgcctttggc
      361 cgcgaggcgg cgcagataag cgctatgctc cagcatcacc gcgcgatcct cctcggtcat
      421 gtcctgggcg aagcttgggc gcggcggcaa caggcgatag acgaacggct tcatctctct
      481 cgacgccttt cttcgtagcc attcttgcga tcctcgccga tcggacgctg ctcgcgcaac
      541 cgaatcttaa ccgcgtcggt ctcaaagtcg tctcatggtc gtcaagacgc gcgtgcgcgc
      601 catcctgctg ccgatcgcct tctatctcgt atcgggcgcc gccagcggct tctttgtctg
      661 gcaagcctcg atcggcgatc gcggcctcca cgcaaaagcg cattatcagg cgcaaatggc
      721 ggcgctgagc gcgcaattga cgagcctgca ggaggagcac aaggccctgc agcacaaggt
      781 cggcctaatg cgcgacaacg ccatcgaccg cgatctgctc gacgaggagg cccgcgccaa
      841 gctcgaccgg gtcgacaaga acgaccttgt cgtcttcacc aatccggtga agctgcgctg
      901 agcggcgctc attcctcgat atgctcgcgc ccgcggcacg gtccccttct caagcgggct
      961 gtaaaagaac gcgcccacgc ttcagggctt ctaccgagga acatcgtccc ggctgtcacg
     1021 tcctgcggct gtccccgaat cgatctctga tcggacccta acaatcatgc gccgcgagac
     1081 gggcggcgcc gggcgctttc catccgccaa atcgtagtct ttgttcgcct gcgccttcgt
     1141 gctatggccg ctggccggcc gcgagcgcgt ttctccgccg gtccttgagg gcaagccatg
     1201 acgatcgctg tcgcgacgcc cgccgacggg cgcattcgct ccaattcctt cttttccgcg
     1261 ccgctcgccg aagccgatcc ggaaatcgcc aaggcggtgg agctggagct cggccgccag
     1321 cgcgacgaga tcgagctgat cgcctccgag aacatcgtct cccgggctgt gctcgaagcg
     1381 cagggctcgg tgatgaccaa cgaatatgcc gagggctatc caggccggcg ctattacggc
     1441 ggctgccaat tcgtcgacat cgccgaaaaa ttggcgatcg agcgggcctg caagctgttt
     1501 tcctgcaaat tcgccaatgt gcagccgaat tccggcagcc aggccaatca gggcgtgttc
     1561 atggccctga tgcagccggg cgacacgttc atggggctcg acctcgccgc cggcggccac
     1621 ctcacgcatg gctcccccgt caacatgtcg ggccgctggt tcaagccggt ctcctacacg
     1681 gtcgaccgcg acagccatcg gatcgatatg gagggggtcg aaaggctcgc ccatcagcac
     1741 aagccgaagc tgatcatcgc cggcggctcg gcctatgccc gccattggga cttcaagcgc
     1801 ttccgcgaga tttgcgacgc ggtcggggcc tatttcgtcg tcgacatggc tcatttcgcc
     1861 ggcctcgtcg ccggcggcgc ccatccgtcg cccttcccgc acgcccatgt cgccacctcg
     1921 acgacgcata agaccctacg cgggccgcgc ggcggcttga tcctcaccga cgacgaagac
     1981 ctcgccaaga agatcaattc ggcgattttt ccgggcctcc agggcggtcc gttgatgcat
     2041 gtcatcgccg ccaaggcggt cgccttcggc gaagcgctga cccaggaatt caagaactat
     2101 gcgcaagccg ttgtcgtcaa cgccaaagcg ctcgccgcgc cgattttggc gggcggctat
     2161 gcgctggtca ccggcggcac cgacaaccat ctgatgctgg tcgacctgcg tccgaaacgc
     2221 ctgaccggca aggccgccga ggcggcgctc ggccgggccc acatcacctg caacaagaac
     2281 ggcgtgccgt tcgatacgga aaagccgacg atcacctccg gcatccggct cggctcgccg
     2341 gcggcgacgt cgcgcggctt tggcgcggcg gaattcgcca gggtgggcga actgatcgtc
     2401 gagacgctcg acggcctggc ctcggcgggc gaatcgggaa atgctgcgac cgaagcgcgg
     2461 gtgcgggcga gcgtcgcgga actgacccgg cgctttccga tctattgatt ctgcgccggc
     2521 cagaggcggc gctggagcga acagccgatg cgatgcccct attgcggcag cctggagacg
     2581 caggtcaagg attcgcgccc gaccgaggat tcctcctcga ttcgccgccg ccgcgtctgt
     2641 cccgattgcg gcggccgctt cacgaccttc gagcgcgtgc aattgcgcga attgacggtg
     2701 gtcaagaaat ccggccggcg ggcgccgttc gaccgcgaca agctgcagcg ctccgtcgaa
     2761 gtggcgctcc gtaagcggcc ggtcgcctcc gaccggatcg agcgcctggt caacggcatc
     2821 gtgcgccagc ttgaaagcca gggcgacggc gatatcccga ccgaccggat cggcgaattg
     2881 gtgatggagg gcttaaaagg ccttgatgat gtcgcttatg tccgcttcgc ctcggtctac
     2941 cgcaatttcc gcgaggcgcg cgacttcaac gccattgtcg gcgaattgtc cgccgagccc
     3001 gagcggcccg caaagccggc cggcaatggg cgcaaggcgc ccgccgagtc ttaaagtgaa
     3061 cgaccgcgcc gctttggacg agcgcttcat ggccgccgct ttggcgctgg gccggcgcgg
     3121 ccttggccgc gcggcgccca atccggccgt cggcgcgctc atcgtgcgcg acggcgtcgt
     3181 cgtcgggcgc ggcttcaccg cggcgggcgg ccggccgcat gccgagcgca gcgcgctcga
     3241 tcaggccggt catttggcca aaggcgcgac aatttacgtg accttggagc cctgcgcgca
     3301 ccagggccgc ggcccgcctt gcgctgcggc gatcgttgag gcggggatcg cctgcgtcgt
     3361 ttcggcgatg gaggaccccg atccgcgcac cgccggtcag ggtcacgctt tgcttcgccg
     3421 ggcgggaatc gaggtcgccg tcggcgtcgg cgccgcagcg gcgcggcggg atcatctcgg
     3481 ccatatcttg cgggtgagtc aaaatcggcc cgcggtgacc ttgaagctgg cccagaccgc
     3541 cgacggcttc gccgccggcg cgcgatacga tccgcgtctc gcgatcaccg gcgaggccgc
     3601 caattcccgg gtgcaaatcc tgcgcgccat gcacgatgcg atcatgatcg ggatcggcac
     3661 ggcgctgggc gacgatccgc tgctcaccgt ccgattccct ggcgccgagg ccaggccgct
     3721 cagggtcgtg ctcgatccgc gcctgagcct gccgccgcg
//
LOCUS       DATGJW010000022         6187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1246642, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000022 DATGJW010000000
VERSION     DATGJW010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6187
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1246642"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..548)
                     /locus_tag="VKU03_00695"
     CDS             complement(<1..548)
                     /locus_tag="VKU03_00695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)(+) transhydrogenase (Re/Si-specific)
                     subunit alpha"
                     /protein_id="HLJ69803.1"
                     /translation="MLIGVVAETDPSETRVAATPETVKKFIALGADVVVETGAGLKSG
                     VTDADYQAAGARLAGAGEVLSQADILLKVRRPTGDELAGIKSGALIVATMDPYGHQDA
                     VDALARAGVAAFAMELLPRITRAQVMDVLSSQANLAGYRAVIDASAEYGRALPMMMTP
                     AGTVAAAKIFIMGVGVAGLQAIA"
     gene            complement(616..900)
                     /locus_tag="VKU03_00700"
     CDS             complement(616..900)
                     /locus_tag="VKU03_00700"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HLJ69804.1"
                     /translation="MADDHSVATAEDVMDMPAHEETYADFLALTEVTVVSLLCILLLL
                     VIWGLEGHPLAAFVGFLLTSAAAAIGLYSGLTWRAVLPVFVLLGLACIVL"
     gene            1056..1874
                     /gene="lgt"
                     /locus_tag="VKU03_00705"
     CDS             1056..1874
                     /gene="lgt"
                     /locus_tag="VKU03_00705"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HLJ69805.1"
                     /translation="MPLFVLPYPLINPILVEIGPFAIRWYALAYIAGLIIGWALIRRT
                     LRDKTLWADAPRPSVDSVDDLLVYCALGVIVGGRLGNVLFYDPGYYFAHPIEIFKVWE
                     GGMAFHGGLIGALIGMALFARRYRAPLLTTFDLVALVAPIGIFLGRIANFIRPELWGR
                     PTDVPWAVIFPGSDGLPRHPSQIYEALLEGLAVFVILQIAARMGALRRPGLATGLFGA
                     AYGVARIFCEFFRDPDPRLEDLGHGLTMGMVLSLPLVIIGICLIVFANRQWRSA"
     gene            1885..2973
                     /locus_tag="VKU03_00710"
     CDS             1885..2973
                     /locus_tag="VKU03_00710"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012322303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLJ69806.1"
                     /translation="MSALGEEIKALIRDHGPISLERYMAMALTHPTHGYYMHRDPFGA
                     SGDFTTAPEISQMFGELIGLWAAEVWASMGAPNPVHLIELGPGRGTLMSDALRAARVA
                     PNFRAVLDVCLIETSPMLAGIQHETLLTAGAPISWVSQWRDAPDGPAIIIANEFLDAL
                     PIRQYVRGQRDWRERLVALDDNGALAFALAANGEPYIGARAEEGAVLEVNAIAHRLLY
                     AIAERLTNQGGAALFIDYGHTVTGFGDTLQAVRAHRMVDPLSEPGTADITAHVDFAAM
                     ARSARAAGAAVYGPIPQGDFLRALGLDQRAATLARKGGPQQAADIEAARSRLAGKGEG
                     EMGALFKAMVLANRNMPPPPGFQPAGFA"
     gene            2970..3752
                     /locus_tag="VKU03_00715"
     CDS             2970..3752
                     /locus_tag="VKU03_00715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphenol oxidase family protein"
                     /protein_id="HLJ69807.1"
                     /translation="MSEGEAEALTSGLLDAPGITHAFFTRRGGVSAGFYATLNGGVGS
                     RDEPGAVAENRARMAAAIGVAPECLLVSYQVHSPDALIVDEPWPPAERPRCDALATAT
                     RGLGLGVTGADCGVLLFADAEAGVIGAAHAGWKGALTGIIEATVAAMARLGAKRGRIA
                     AALGPTIRQDSYEVGPEFLARFLDADAAYARFFKESARSGRAMFDLPGFIAARVAQAE
                     IGRFEDLKLDTYADDERFFSYRRATHRGEADYGRLVAAIALR"
     gene            complement(3777..4436)
                     /locus_tag="VKU03_00720"
     CDS             complement(3777..4436)
                     /locus_tag="VKU03_00720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ69808.1"
                     /translation="MAPIREPAELVGRTMKITKQQAEHNRARVVETAARLFREKGFEG
                     VGVADLMRAAGLTHGGFYNHFESKDELSAAACAHALAQAVAAIESVAESQPAKAEALG
                     EYRRRYLSHKSRDAEGFRCPMVAFGSDVSRQGPELRDVYAGGLKRYLDAFVRAYASER
                     GGRRAEADLRAEAIAHFATMVGAVSLARSVAKADPVLSDEILDATLANLEGAKVKRRR
                     R"
     gene            4552..5847
                     /locus_tag="VKU03_00725"
     CDS             4552..5847
                     /locus_tag="VKU03_00725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011503698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ69809.1"
                     /translation="MLAEALARRLARRGVHYGWAIAAVTFLTMLTTAGAVGLPGAFIL
                     PLSKEFGWDAAQISGPLAVRLILFGLMAPFAAALIERFGVRNVVVSAIGLIVAGLLLA
                     LTMSAIWQLFIYWGIVIGVGTGLTAMVFAAIVSNRWFNQRRGLVLGMLTAANSTGQLV
                     FLPLAAWLIDHVGWRYALAPSIGALVLAGLLILLLMRDRPSDLGLAPYGDPPLAPGGA
                     PAPPAPAPAIGRAFSVLAECSRSGAFWVLFFTFYVCGLSTNGLIQTHFISFCVDFGLP
                     AVAAASTLAMMGLFDIAGTIGSGWLSDRYDSRALLFWYYGLRGLSLLYLPYSGFTVYG
                     LSIFSVFYGLDWIATVPPTVKLATQTFGKEKAAVAFGWIFAAHQLGAATAAFGAGFSR
                     TEWSTYVPAFLIAGAACLFASASVWLIGARKQAPATAAA"
BASE COUNT          914 a         2120 c         2021 g         1132 t
ORIGIN      
        1 gcgatcgcct gcaggccggc gacgccgacg cccatgatga atattttcgc cgccgccacc
       61 gtgccggccg gcgtcatcat catcggcaag gcgcggccat attccgccga tgcgtcgatc
      121 accgcgcgat agccggcgag attggcttgg ctcgacagca cgtccatgac ctgggcgcgg
      181 gtgatgcgcg gcagcagctc catggcgaac gcggcgacgc ccgctcgcgc cagcgcgtcg
      241 accgcgtcct gatggccgta agggtccatg gtcgcgacga tcagcgcgcc gcttttgatt
      301 ccggccagtt cgtccccggt cggccggcgc actttgagga ggatgtcggc ttgggaaagg
      361 acctcgccgg cgccggcgag gcgcgcgccg gccgcctgat aatccgcgtc ggtgacgccc
      421 gatttgagcc cggcgcccgt ctcgacgacg acatccgcgc ccagcgcgat gaatttcttg
      481 accgtctcgg gtgtcgccgc gacgcgcgtc tcagacggat cggtctcggc gacaaccccg
      541 atgagcatgg ctgacaccct aaaggaagag gcgcggaccg aagcggcgtc ccgccctgcg
      601 ctggcgagaa ggcggctaga ggacgatgca ggcgaggccg agcaggacga agacggggag
      661 aacggcgcgc caggtcaggc cggaatagag gccgatcgcc gccgccgcgc tggtcagcag
      721 gaagccgaca aacgccgcca gcggatggcc ctccaagccc cagatgacca gcagcagaag
      781 gatgcacaac aggctgacga ccgtgacttc ggtcagagca aggaaatcgg cgtaagtttc
      841 ctcatgcgcc ggcatgtcca tgacgtcttc cgccgtcgca accgaatgat cgtccgccat
      901 accccgtccc tttaggagaa gccgttagag ctgaaatcgc ctttaccgga aaggcggcgg
      961 cttcgcaacg tgacaatccg cgccgcgccc gcggcgcggc tgagcgcgcg aaccgcttga
     1021 cgccgagccg cgacgcagcg catcacgccc cgtccatgcc gcttttcgtt ttgccctacc
     1081 cgctgatcaa tccgatcctg gtcgaaatcg gccccttcgc catccgctgg tatgcgctcg
     1141 cctatattgc cgggctgatc atcggctggg cgttgatccg gcggaccttg cgcgacaaga
     1201 cgttgtgggc ggacgcgccg cggccgagcg tcgacagcgt cgacgacctg ctcgtctatt
     1261 gcgcgctcgg cgtgatcgtc ggcggccggc tcggcaatgt ccttttctac gatcccggct
     1321 attatttcgc tcacccgatc gagattttca aagtttggga aggcgggatg gcctttcatg
     1381 gcggcttgat cggcgccttg atcggaatgg ctcttttcgc ccgccgctac cgcgcgccgc
     1441 tcctgacgac cttcgatctt gtcgcgctgg tcgcgccgat cggcattttc ttgggccgca
     1501 tcgccaattt cattcgcccc gaactctggg gccggccgac cgacgttccc tgggcggtga
     1561 tctttcccgg ctccgacggc ctgccgcgtc atccgagcca gatctacgag gctttgctcg
     1621 aagggctcgc cgtcttcgtc attttgcaga tcgccgcgcg catgggcgcg ctgcgccgtc
     1681 ccggcctcgc caccggtctt ttcggcgcgg cttacggcgt cgcgcgcatc ttttgcgagt
     1741 tctttcgcga tcccgatccg cggctggaag atctcggcca cggcttgacc atggggatgg
     1801 ttttgtccct gccgctcgtc ataattggga tttgcctgat agtctttgcg aatcgccaat
     1861 ggcggagcgc ataggggagc gcagatgagc gcgctggggg aggagatcaa agccttgatc
     1921 cgcgatcatg gcccgatctc gctcgagcgt tacatggcga tggcgctcac ccacccgacg
     1981 catggctatt acatgcatcg cgatccattc ggcgcgtccg gcgatttcac caccgcgccg
     2041 gaaatcagcc agatgtttgg cgaattgatc gggctctggg ccgccgaagt ctgggcgtcg
     2101 atgggcgcgc cgaaccccgt ccatctgatc gagctcgggc cgggacgcgg caccctgatg
     2161 agcgacgcgc tgcgcgccgc gcgcgtcgcg ccgaatttca gggccgtgct cgatgtctgc
     2221 ctcatcgaaa cgagcccgat gctggcggga atccagcacg agactctgtt gaccgccggc
     2281 gcgccgatct cctgggtttc gcaatggcgg gacgcgccgg acggcccggc gatcatcatc
     2341 gccaacgagt ttctcgacgc tttgccgatt cgccaatatg tgcgcggcca gcgcgattgg
     2401 cgcgagcggc tcgtcgcctt ggacgataac ggcgccttgg ccttcgcctt ggcggcgaat
     2461 ggcgagccct atattggcgc gagggccgaa gaaggcgcgg tcctggaagt gaacgcgatc
     2521 gcccatcgtc tcctctacgc gatcgccgaa agactgacga accaaggcgg cgcggccttg
     2581 ttcatcgatt acggccatac ggtgaccggc ttcggcgaca ctttgcaggc ggtgcgcgcg
     2641 catcgcatgg tcgatccgtt gagcgagccg ggcaccgccg acatcaccgc ccatgtcgat
     2701 ttcgccgcga tggcgcgcag cgcccgcgcc gctggcgcgg cggtctatgg gccgatcccg
     2761 caaggggact ttctgagagc tttgggcctc gatcaaaggg cggcgacgct cgcccgcaag
     2821 ggcggtccgc aacaggccgc cgacatcgag gcggcgcgtt cgcgtctcgc cggcaagggc
     2881 gagggcgaga tgggcgcgct gttcaaggcg atggtcctcg ccaaccgcaa catgccgccg
     2941 ccgcccggct tccagcccgc gggcttcgca tgagcgaggg cgaggcggaa gccttgacca
     3001 gtggcctcct cgacgcgccg ggaatcacgc acgccttctt cacccgtcgc ggcggcgtgt
     3061 cggccggctt ctacgccaca ctcaatggcg gcgtcggctc gcgcgacgag ccgggcgcag
     3121 tggctgaaaa tcgcgcccgc atggccgcgg cgatcggcgt cgcgccggaa tgcttgctcg
     3181 tctcctacca ggtccattcg ccggacgcgc tcatcgttga cgaaccctgg ccgccggccg
     3241 agcggccgcg ctgcgacgcg ctggcgaccg cgacgcgcgg tctcggcctc ggcgtcaccg
     3301 gcgccgattg cggcgtcctg ctcttcgccg acgcggaggc cggcgtgatc ggcgccgctc
     3361 atgccgggtg gaaaggcgcc ctgaccggaa ttatcgaagc gaccgtcgcg gcgatggcgc
     3421 ggctcggcgc caagcgcggc aggatcgccg cggcgctcgg cccgacgatc cggcaagaca
     3481 gttacgaagt cggccccgaa tttctcgcgc gattcctcga cgcggacgcc gcttacgcgc
     3541 ggtttttcaa agaaagcgct cgctctggcc gcgcaatgtt cgatttgccg ggctttatcg
     3601 cagcgcgcgt cgcgcaagcc gagatcggac ggttcgagga cctgaagctc gacacctacg
     3661 ccgacgacga gcggtttttc agctaccgcc gcgcgaccca tcgcggcgag gcggattacg
     3721 ggcggctggt ggcggcgatc gcgctgcggt gaaggcctcg gatccctttg cgactcttat
     3781 cgcctccgcc gcttgacctt cgcaccttcc agattcgcca gcgtcgcatc gagaatctca
     3841 tccgacaaaa cgggatcggc cttggcgacg gagcgggcga gcgacaccgc cccgaccatc
     3901 gtcgcgaaat gagcgatcgc ctcggcgcga aggtcggcct cggcgcgccg gccgccgcgc
     3961 tcgctcgcat aggcccgaac gaaagcgtcg agatagcgct tcaagccgcc cgcgtagacg
     4021 tcgcgcaatt ccggtccttg ccgcgacacg tcactgccga acgccaccat cggacagcga
     4081 aagccttccg cgtcgcggct cttatgcgaa agataccgcc gccggtactc gccgagcgct
     4141 tccgccttcg ccggctggct ttccgcgacc gattcgatcg ccgcgaccgc ttgcgccaaa
     4201 gcgtgggcgc aggcggcggc gctcagttcg tccttggatt cgaaatgatt gtagaagccg
     4261 ccatgggtga ggccagccgc gcgcatgagg tcggcgacgc cgaccccttc aaaacctttc
     4321 tcgcggaaaa gccgcgccgc ggtctcgacg acgcgggcgc gattgtgctc cgcctgctgc
     4381 ttggtgatct tcatcgttcg gccaacgagc tctgccggtt cgcgaatagg cgccatgcgc
     4441 cggcgccctt gactaatgat gacgaatatc atcatttaat gatgactgtc atcattaaaa
     4501 tggcgtttcg ccgcgcttcg cgcggctggc gcgcccgaaa aggagcgagg catgctggct
     4561 gaggctttgg cgcgaaggct ggcgcggcgc ggcgtccatt acggctgggc gatcgcggcg
     4621 gtgacgttcc tcaccatgct gaccaccgcc ggcgcggtcg gcctgccggg cgcgttcatt
     4681 ctgccgctgt cgaaggagtt cggttgggac gcggcgcaga tttccgggcc gctcgccgtg
     4741 cggctgatcc tgttcggcct gatggcgccg ttcgccgcag ctttgatcga gcgcttcggc
     4801 gtgcgcaacg tcgtcgtctc ggcgatcggc ttgatcgtcg ccggcctgct gctcgccctg
     4861 acgatgagcg cgatctggca attgttcatc tattggggga tcgtcatcgg cgtcggcacc
     4921 ggccttaccg ccatggtctt cgccgcgatc gtctccaacc gctggttcaa tcagcggcgc
     4981 ggcctcgtgc tcggcatgct gacggcggcc aactcgaccg gccagctcgt cttcctcccg
     5041 ctcgccgcct ggctcatcga tcatgtcggc tggcgctacg ccttggcgcc gtcgatcggc
     5101 gcgctggtcc tcgccggctt gctgattctt ctgctgatgc gcgaccggcc gtcggatctc
     5161 ggccttgcgc cctatggcga tccgccgctc gcgccgggcg gcgctcccgc gccgcccgcc
     5221 cccgccccgg cgatcggccg cgccttctcg gttctcgccg aatgttcacg gagcggcgcc
     5281 ttctgggtcc tgttcttcac cttctatgtc tgcggcctca gcaccaacgg cctgatccag
     5341 acccacttca tctcgttctg cgtcgatttc ggcctgccgg cggtcgccgc cgcctcgact
     5401 ctggcgatga tgggcctgtt cgatattgcc ggtacgatcg gctcgggctg gctctccgac
     5461 cgctatgaca gccgggcgtt gctgttctgg tactacggct tgcgcggcct gtcgctgctt
     5521 tacctgccct attccgggtt cacggtttat gggctgtcga tcttctcggt cttttacggc
     5581 ctcgattgga tcgcgaccgt gccgccgacc gtcaagctcg ccacccagac gttcggcaag
     5641 gaaaaggcgg ccgtcgcttt cggctggatt ttcgcggctc accagctcgg cgcagccacg
     5701 gcggctttcg gcgccggctt ctcgcggacc gaatggagca cctacgttcc ggccttcctc
     5761 atcgccggcg cggcgtgcct gttcgcgtcg gcgtcggtct ggctgatcgg cgcgcgcaag
     5821 caagcgcccg ccacggcggc ggcgtaaccc cttctccccg tttgcgggga gaaggtggcg
     5881 cgaagcgccg gatgaggggc tgcgcaggac tcgaaaagta cgcacccttc gaaggaagct
     5941 aatcggcagg ttcggcccct catccggccg gcttcgccgg ccaccttctc cccgctgcgc
     6001 ggggagaagg atgtctgcac gcccctcagt gcagcgcgac gccgctcttg gcgtcgaaga
     6061 ggtgaattcg ctccggcggc gcggcgagct tgatcgtgcc gccgggccga gcgttgaccc
     6121 gctcgcggaa cacgctaacg atctcgcggc cgcagaattt cgcgaagacc tgcgtctccg
     6181 agcccgt
//
LOCUS       DATGJW010000023         6941 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1350972, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000023 DATGJW010000000
VERSION     DATGJW010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6941)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6941)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6941
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1350972"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            177..401
                     /locus_tag="VKU03_00730"
                     /pseudo
     CDS             177..401
                     /locus_tag="VKU03_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429118.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
     gene            492..2051
                     /locus_tag="VKU03_00735"
     CDS             492..2051
                     /locus_tag="VKU03_00735"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLJ69810.1"
                     /translation="MRRTGLTRAVQPAGAAKDDPPPRLATRSGGEAPLGALARPPQAL
                     AQDRAVLLAIATTTLIGLALRLWCAESPLWLDEIWSLDNLAGLSHGWQVFWRISHDNN
                     HFLNSLWLFWVSGDSHDPLLLRAPSIAMGAALIPVMAAILWRRGGAAAVAAALLTACS
                     YFFVSYSAEARGYAGEALALAAAYLCLERAMADPRSPARFGLAAAAGLGMFWHLATLP
                     ALILLGFACLAELRRREGRWDTALHSAFHLFAPVGLAVLPALACLAAGILVTGKFTIG
                     GLRPFAYGPAFAAMGSAADQTLGLPETAPALATLAGAASAFALGLIFRLVAEERRIVY
                     GVFLIGAPAAVLLLRPANAHIARYYLPCALFLILFAAEAAATLWRQGGLRRWAAALAV
                     AASLTGGAAALSARQNSLSAAWPQALVTIEASGKRVVASSADQRTARLIGEFNRAHAP
                     PIALAARPDWCAKRPDWLLAEPASVEGEAPQIEIGPCRLRFVFVGAFGGGGLTAVDWL
                     LYRRAADEDGH"
     gene            complement(2121..2834)
                     /gene="rplA"
                     /locus_tag="VKU03_00740"
     CDS             complement(2121..2834)
                     /gene="rplA"
                     /locus_tag="VKU03_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L1"
                     /protein_id="HLJ69811.1"
                     /translation="MAHQGKRIRKAVEGIERTKLYKLEEAVKLVKERAVAKFDETVEI
                     ALNLGVDPKHADQMVRGVVNLPNGTGRTLRVAVFARGAKADEAKAAGADIVGAEDLVN
                     TVNGGEINFDRCIATPDMMPLVGRLGKVLGPRGLMPNPKVGTVTMDVAAAVKASKGGA
                     VEFRVEKAGIVQGSVGKASFGEDKLAENIRAFVDAVNKAKPTGAKGTYLKRVSISSTM
                     GPGVKLDLGSVSTATAVGA"
     gene            complement(2839..3285)
                     /gene="rplK"
                     /locus_tag="VKU03_00745"
     CDS             complement(2839..3285)
                     /gene="rplK"
                     /locus_tag="VKU03_00745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11"
                     /protein_id="HLJ69812.1"
                     /translation="MAKKVAGYIKLQVPAGAANPSPPIGPALGQRGLNIMEFCKAFNA
                     RTAQMEKGAPIPVVITAYQDRSFTFEMRQPPVTFFLKKAAGLKLGKKPASGAKTPGRG
                     FVGKVTKEQVREIAEKKIVDLNCSTIDAAMAMIEGSARSMGLEVVE"
     gene            3454..3693
                     /locus_tag="VKU03_00750"
     CDS             3454..3693
                     /locus_tag="VKU03_00750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69813.1"
                     /translation="MPAITVRNLSAETLRALKALAARHGRSAEAEIRSILEAAARGEP
                     RVKLGSLLVEIGREFGGADLDVKRDKIVTEPLDFE"
     gene            3816..5015
                     /locus_tag="VKU03_00755"
     CDS             3816..5015
                     /locus_tag="VKU03_00755"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLJ69814.1"
                     /translation="MRPASSDGGREEPAIARRAGGGAAPASDKAAKLLHSAKRTTLII
                     AAAYLYDTLILFGYWAAGFVDIAVPVAVFALLAALIGAVALAHATGWSLGRRDPTLFL
                     PHQLYAIAVALGVALVAPQIGFQPFATLFAISAFSFMAPNARSLIIAWVAVAIGAAAV
                     IFLVGPRLAVPASTLAGQALTSAVIAGLLARCIWIAMFVRTLRGRLAEKNRSLTAAMA
                     RIETLATTDEVTGLPNRRAIAESLREHMALSRRAGLPLSLAFVDIDHFKRINDAYGHL
                     AGDGALQLIAGALRVGVRESDRVGRFGGEEFLIVMPSTALKEAREPLERLREDVARLD
                     WSSIGLDVQATVTIGAAQYAPGEALEALVRRADLALYLGKETGRNRVVLNPPVEQSGP
                     RRRALSA"
     gene            complement(5019..5357)
                     /locus_tag="VKU03_00760"
     CDS             complement(5019..5357)
                     /locus_tag="VKU03_00760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69815.1"
                     /translation="MSPFNLVQLAAATGIFLVAASSAKGWALSPSLARAVLTLALYSA
                     GNLLMMRLVRSLGMATAFSLSAVIQLIAINVVAILAFGEKLGWAQSAGVALGVVAVAL
                     ITLGPSLTPP"
     gene            complement(5467..5871)
                     /locus_tag="VKU03_00765"
     CDS             complement(5467..5871)
                     /locus_tag="VKU03_00765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ69816.1"
                     /translation="MIVIDSSALVAIFLNEPERQVFEDIVADEDRCVMSAVNVHETIC
                     VLRTRLGPKAVERFWQMLQDSQIEIFPFDEVQVRAAADAFDRYGKGVNSKSKLNLADC
                     AAYALATTMNSRLLFKGRDFSETDVRTAFEGR"
     gene            complement(5868..6281)
                     /locus_tag="VKU03_00770"
     CDS             complement(5868..6281)
                     /locus_tag="VKU03_00770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69817.1"
                     /translation="MARSGWVQPDLAEISDWSKRPDRRVLRPPRRAAAIWAISGRACY
                     KARMIALSAETEALAERLAAAQRLPVAEAVRLAIEEKARIAGIAPGPSRPRDQTAAAI
                     AARRAALDRFVAEISSMPVRDARSPCEIIDDLNAL"
     gene            6280..6711
                     /locus_tag="VKU03_00775"
     CDS             6280..6711
                     /locus_tag="VKU03_00775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010971569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HLJ69818.1"
                     /translation="MAHAYDPNNIFAKILRGEAPCVKVFEDETALAFMDIMPRGEGHI
                     VIVPKIAARNILDIAPADLARFMPSVQKVAVAAKDGLNAEGVKIEQYNEPAAGQTVYH
                     LHFHILPRWTGVALKPPGGPMVSADVLAPIAEKIRAAIARE"
BASE COUNT         1075 a         2359 c         2225 g         1282 t
ORIGIN      
        1 aggatttttt gaccgcttcg cgcaaagttg ttcccgcccc gtcacgcccg ccccgcccct
       61 gtccgctcct tgaggaaggc gatgacgcgc tgctcggccg ccttggtcgg gccggtctcc
      121 cgcatgtgca gggtcaacac ggaatggggc gactgtttgg acggggccgc gtcggcctta
      181 tccaattgcg cgtgctcgaa cgcgacgggc tggtccagcg cacggcctat cctgtcattc
      241 cgccgcgggt cgagtacaaa ttgaccgacc tcgggcggga attgattggc ctgctcgacg
      301 ccatcctgcg ctggtccgag cggcacgcgc ccgaaatcct caaagcgcgc gaacttgcgc
      361 gaaggcgcag ccaggatcgc gagagcgacg cccaggcctg agcgcgcggc ttctcgggag
      421 gcgcctgtcc tcgggccttt attaattttt gcgctttagc gttctgccgt gcgcggcaaa
      481 gcgaacccat catgcgccgg acagggctga cgcgagcggt gcaaccggcc ggcgccgcta
      541 aggatgatcc gccgccgcgc ctcgcgacgc gttccggcgg agaagcgccg ctcggcgcct
      601 tggcgcgacc gccccaagcc ctggcccagg accgcgcagt ccttctcgcc atcgccacga
      661 cgaccctgat cggcctggcg ctccgcctat ggtgcgcaga aagcccgctc tggctcgatg
      721 aaatctggtc cctcgacaat ctggccgggc tttcccatgg ctggcaggtc ttctggcgca
      781 tctcgcacga caacaatcac ttcctgaatt cgctctggct gttctgggtt tctggcgact
      841 cgcacgatcc gctgctcctg cgcgcgccgt cgatcgcgat gggcgccgcg ctgatcccgg
      901 tcatggcggc gatcctctgg cggcgcggcg gcgcggccgc cgtcgccgcc gccctgctga
      961 ccgcctgctc ctatttcttc gtcagctaca gcgccgaagc gcgcggctat gccggcgagg
     1021 cgttggcgtt ggccgccgct tacctgtgtc tcgaacgggc catggccgat ccgcggtcgc
     1081 ccgcccgttt cggtctcgcc gcggccgctg ggctcggcat gttctggcac ctcgccacgc
     1141 tgccggcgct tatcctcctc ggcttcgcct gtctcgccga attgcggcgg cgggagggcc
     1201 gctgggacac ggcgctgcac agcgcattcc atcttttcgc gccggtcggc ttggccgttc
     1261 taccggctct cgcctgcctc gccgccggca ttctcgtcac cggcaaattc accattggcg
     1321 gccttcgccc cttcgcctat gggccggctt tcgccgccat gggctccgcg gccgaccaga
     1381 ctctcggcct gccggaaacc gctcccgctc tggcgacgct tgccggcgcc gcgtcggcgt
     1441 tcgcgctcgg ccttatcttc cggctcgtcg ccgaggagcg gcgcatcgtt tatggcgtct
     1501 ttctgatcgg cgcgccggcc gccgtcctgc tgttgcgccc ggccaacgcc catatcgcgc
     1561 gctactacct gccttgcgct ctgttcctga tccttttcgc ggcggaagcg gcggcgaccc
     1621 tatggcgtca gggcggcttg cggcgctggg cggcggcttt ggccgtcgcc gcctcgctga
     1681 ccggcggcgc ggccgcgctg agcgcccgcc agaactcgct cagcgcggcc tggccgcagg
     1741 cgctggtcac gatcgaagcc tccgggaaac gcgtcgtcgc gagcagcgcc gaccagcgga
     1801 cagcgcgact gatcggcgag ttcaaccgcg ctcacgcgcc gccgatcgcg ctcgccgcac
     1861 ggccggattg gtgcgcgaag cggccggatt ggctgctcgc cgaaccggcg agcgtcgagg
     1921 gcgaggcgcc gcaaatcgag atcgggccct gccggctgcg ctttgttttt gtcggcgcct
     1981 ttggcggcgg cgggctgacc gccgtcgact ggctgctcta tcgccgcgct gcggatgagg
     2041 acggccacta acgaaaaggc ctcgcgcgaa cgcgaggcct ttagcgacaa taagttgtca
     2101 aaaaaagcga agagtcagct ttaagcgccg accgccgtcg ccgtcgagac cgagccgagg
     2161 tcgagcttga cgcccgggcc catcgtcgag gaaatcgaca cacgcttgag gtacgtgccc
     2221 ttggcgccgg tcggcttggc cttgttcacc gcgtcgacaa aggcgcgaat attctcggcg
     2281 agcttgtcct cgccgaacga ggccttgccg accgacccct ggacaatgcc ggccttttcg
     2341 acccggaact cgacggcgcc gcccttcgag gctttgaccg ccgcagcgac gtccatcgtc
     2401 acggtgccga ctttcggatt cggcatcagg ccgcgcggtc caagcacttt gccgagccgg
     2461 ccgaccagcg gcatcatgtc gggcgtcgcg atgcaacggt cgaaattgat ctcgccgcca
     2521 ttgaccgtat tgaccaggtc ctcggcgccg acaatgtcgg cgcccgccgc cttggcttcg
     2581 tccgccttgg cgccgcgggc gaagaccgcg acgcgcaggg tgcggccggt gccgttcggc
     2641 aggttgacga cgccgcgcac catctggtcg gcgtgcttgg gatcgacgcc aagattgagg
     2701 gcgatctcga ccgtctcatc gaatttcgcg acggcgcgct ccttgacgag cttcaccgcc
     2761 tcttcgagct tgtagagctt ggtccgctcg attccctcga ctgctttacg gattctcttg
     2821 ccttgatgcg ccatggccct actccaccac ttccaggccc atcgagcggg cggagccttc
     2881 gatcatcgcc atcgccgcat cgatcgtcga gcaattgaga tcgacgatct tcttctcggc
     2941 gatttcgcgc acctgttctt tcgtcacctt gccgacaaag ccgcggcccg gcgtcttggc
     3001 gccggaggcc ggcttcttgc cgagtttcag gcccgccgcc ttcttgagga agaaggtcac
     3061 cggcggctgg cgcatttcga aggtgaagga gcggtcctga taggcggtga tcacgaccgg
     3121 gatcggcgcg cccttctcca tctgcgccgt gcgcgcattg aaggccttgc agaactccat
     3181 gatgttgagg ccgcgctggc cgagcgcggg accgatcggc ggcgagggat tggccgcccc
     3241 cgccggtact tgcagtttga tatagccggc tactttttta gccattggcg ctgctccaaa
     3301 atgagcgcgg ccgaagcctg gcaaggctcg gctcgcgccg ttgcagttgc gtggtgcgag
     3361 cggtttttcc cggatggctt gccgggcgct ctcccacgcg aggaagcgcg gcgcttagca
     3421 ggtctggtct gaagccgcaa ggaatgatcg caaatgccgg ctattaccgt gcgcaacctc
     3481 tccgccgaaa cccttcgcgc tctgaaagcg ctcgccgctc ggcatgggcg gagcgccgag
     3541 gccgaaatcc gcagcatcct tgaagcggcg gcgcgcggcg agccgcgcgt caagctcgga
     3601 tcgctgcttg tcgagatcgg ccgcgaattc ggcggagccg atcttgatgt caaacgtgat
     3661 aagatcgtga ccgaacctct tgattttgaa tgacattgct caatacgtcg tcacccggct
     3721 cgccattgcg gcgattcgct cgaccctgct aagggtttgt tattgatgcg gctccacaat
     3781 catctccaac gtgcggttct cgcgaggttt ggagtttgcg tccggcgtcg tccgatggcg
     3841 gcagagaaga gccggcgata gcgcgccgag ccggcggcgg cgccgcgccc gcgtcggata
     3901 aggccgccaa gctgctgcac agcgccaagc gcacaacgct gatcatcgcc gctgcttatc
     3961 tttacgatac gctgatcctg ttcggctatt gggcggcggg ctttgtcgac atcgccgtgc
     4021 cggtcgccgt ctttgctctt ttggcggcgc tgatcggcgc tgtggccttg gctcatgcaa
     4081 ctggctggag cctgggccgg cgcgacccga ccctgttcct gccgcaccag ctctacgcga
     4141 tcgccgtcgc cctcggcgtc gcgctcgttg ccccgcagat cggattccag cccttcgcca
     4201 cgcttttcgc gatcagcgcg ttcagcttca tggcgcccaa cgcccgaagc ctgattatcg
     4261 cctgggtggc ggtggcgatc ggcgcggcgg cggtgatctt tctcgtcggc ccgcgcctcg
     4321 ccgtgccggc ctcgaccctg gccggccagg ctttgaccag cgcggtgatc gccggcctgc
     4381 tggcgcgctg catctggatc gcgatgttcg tgcgcacgct gcgcggccgg ctcgccgaga
     4441 agaaccgctc gctgacagcg gcgatggcgc ggatcgaaac gctggccacg accgacgagg
     4501 tcactggcct gcccaatcgg cgcgccatcg ccgagagcct gcgcgagcat atggcgctta
     4561 gccggcgcgc cggcctgccg ctatcgctcg ctttcgtcga tatcgaccat ttcaagcgga
     4621 tcaacgacgc ctatggccat ctcgccggcg acggcgcgct tcagctcatc gccggcgcgt
     4681 tgcgcgtcgg cgtccgcgaa agcgaccgcg tcggccgctt cggcggcgaa gaattcctca
     4741 tcgtcatgcc atcaacggcc ttgaaggagg cgcgcgagcc gctggagcgg ctgcgcgagg
     4801 acgtcgcccg gctcgattgg tcctcgatcg ggctcgacgt ccaggcgacg gtgacgatcg
     4861 gcgccgcgca atatgcgccc ggcgaggcgc tcgaagcctt ggtgcggcgc gccgatctcg
     4921 cgctctatct cggcaaggag accggccgca accgcgtcgt gctcaatccg ccggtcgagc
     4981 agagcgggcc gcggcggcgc gcgctgagcg cttaaacatc agggcggggt cagagacggg
     5041 ccgagcgtga tcagcgccac cgcgacgacg ccgagcgcga cgccggcgct ttgcgcccag
     5101 ccgagcttct cgccgaaggc taaaatcgca accacattga tggcgatgag ctgaatcacg
     5161 gcggaaaggc tgaaggctgt cgccatgccg agcgagcgga ccaatcgcat catcaaaaga
     5221 ttgccggccg aatagagcgc gagggtaagc acggcccggg cgaggctcgg cgacagcgcc
     5281 cagcctttcg cgctgctcgc cgcgacgagg aaaatcccgg tcgcggcggc gagttgaacg
     5341 aggttgaacg gactcatcgg cgcgcctccc tgccgctcac gtagcattgc ggcggcggcg
     5401 cctcaacgac gagggctggt cgcggcgcgg acgcttggcg aactcggccg ccgcaaagcg
     5461 tggcggtcag cgtccttcga acgccgtcct cacatctgtt tcggagaaat cgcgcccttt
     5521 aaacaagagt cgcgaattca tggtcgtcgc gagcgcatag gccgcgcagt cggcgaggtt
     5581 gagctttgat tttgaattga cgcccttgcc gtagcggtcg aaggcgtcgg ccgccgcgcg
     5641 aacttgaacc tcgtcaaatg gaaagatctc gatttggctg tcctggagca tctgccaaaa
     5701 ccgctcgacc gctttggggc cgaggcgcgt gcggagcacg catatcgtct catgcacatt
     5761 caccgccgac atgacgcaac gatcttcgtc cgcgacgatg tcttcgaaga cttgcctctc
     5821 gggctcgttg agaaaaatcg caacaagcgc cgaactgtcg atgacgatca tagagcgttc
     5881 agatcgtcga tgatctcgca cggcgagcgg gcgtcacgca ccggcatcga cgatatttcc
     5941 gcgacaaatc gatccagcgc ggcccgccgg gcggcgatag cggcggcggt ttggtcgcgc
     6001 ggccggctcg gcccgggcgc aatgcctgcg atccgcgctt tttcctcaat agcaagtctc
     6061 acagcttccg caacgggaag gcgttgcgcc gccgccaagc gctcggcgag cgcttcggtt
     6121 tccgcagata gagcgatcat gcgggcttta tagcacgccc ggcctgaaat tgcccatatc
     6181 gctgcggcgc gcctgggtgg tcgcaggacg cggcgatcag gacgcttgct ccagtccgaa
     6241 atctcggcaa gatcgggttg cacccatccc gagcgcgcca tggcccacgc ttacgacccg
     6301 aacaacatct ttgccaaaat cctgcgcggc gaagcgcctt gcgtcaaagt cttcgaggac
     6361 gagaccgccc tcgccttcat ggacatcatg ccgcgcggcg agggtcacat cgtgatcgtt
     6421 ccgaaaattg cggcccgcaa cattctcgac atcgcgccgg ctgacctcgc ccgtttcatg
     6481 ccgtcggtac agaaggtagc ggtcgccgcg aaggacggcc tcaacgccga aggcgtcaag
     6541 atcgagcaat ataacgagcc ggccgccggc cagaccgtct accatctgca tttccatatc
     6601 ctgccgcgct ggaccggcgt cgcgctcaag ccgccgggcg gaccgatggt gagcgcggac
     6661 gttctggcgc cgatcgcgga gaagatacgc gcggcgatcg ctcgcgagta attggagtcg
     6721 tcattgcgag cgtagcgacg caatccagga acgggattgc tttcgtcgct ttcgctcctc
     6781 gcagcgacgg acgcgatttg aatttctttt cacacaagtt cgtacttcgc gccagcaaga
     6841 cggcgtgatc agcccatctt ccgccggtcg ctccgctatt ttgaccccca gtgaaattcc
     6901 gcttgacagg caactcacgg gttgcctata taaaggcaac c
//
LOCUS       DATGJW010000024         4666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1455143, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000024 DATGJW010000000
VERSION     DATGJW010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1455143"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VKU03_00780"
     CDS             complement(<1..262)
                     /locus_tag="VKU03_00780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69819.1"
                     /translation="MVAGALRALDFKGRLSAPEGLRAYAAKAAPSERDFRGIRRRVAV
                     SYVSIRTKEIVTLTPRRVAGAQVLYLHGGGFVLPPHNQHWRFL"
     gene            803..1570
                     /locus_tag="VKU03_00785"
     CDS             803..1570
                     /locus_tag="VKU03_00785"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLJ69820.1"
                     /translation="MNEKKSKPRRLAFGVDPKRPQRYSLRQARYDALAHDIDALAAEA
                     ARQGRRLSVLDIGAGGGASPLHLRARPNFATIDLDGADRDRQYNVDESLYRRFFIADL
                     TEGCPELSSESYDVVICEQVLEHIHELRKPMETIGRLVKPGGKAFVGVPIFLPPLHLA
                     RRHLVPRLDRLVRSTHRRDHVQAFTLASVKGALLAYSKLSFVEARGFRIISGGILRPL
                     EERRAWWAFNRWLGARIPAACIEVQIVLEKPAASQKA"
     gene            1567..2208
                     /locus_tag="VKU03_00790"
     CDS             1567..2208
                     /locus_tag="VKU03_00790"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HLJ69821.1"
                     /translation="MIARLPRFSPGQRIGLLGGSFNPPHQGHLLISLFALKRLRLDRV
                     WWLATPGNPLKDNEGLPSLAKRLAEASAVARDPRIAVTGFEAEIGARFTADTVAFLSR
                     HAPGVSFVWLMGADNLRQFHLWRRWRRLAEQVPVAAIARPGSTLTALAAPAAGYFAKF
                     RVPEARAADFAGSRPPAFIFLHGPQSMLSSSAIRRDEAVANRVEKRAETAHVL"
     gene            2250..2672
                     /gene="rsfS"
                     /locus_tag="VKU03_00795"
     CDS             2250..2672
                     /gene="rsfS"
                     /locus_tag="VKU03_00795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HLJ69822.1"
                     /translation="MTLAQPQNAAVEPRPPASRSVESEAGFLAQTITTSLEDAKAEDI
                     VSIDLRGKTTLADLMVIATGRSNVHVGAIADRLLKAFKAGGLAAPRIEGLPNGDWVLL
                     DASDAIVHIFRPEVRKFYNLEKMWGADRPGEKRPARAT"
     gene            2703..3185
                     /gene="rlmH"
                     /locus_tag="VKU03_00800"
     CDS             2703..3185
                     /gene="rlmH"
                     /locus_tag="VKU03_00800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (pseudouridine(1915)-N(3))-methyltransferase RlmH"
                     /protein_id="HLJ69823.1"
                     /translation="MRLLLIAVGRLKAGPEREWFERYAKRLADMARAVGIASFEWREI
                     EEGRARRAEERRAEEARAIRAALPAGGLLVVCDERGKALTSAQFAAEIGAARDRGEVA
                     YALVLGGPDGLEPKLRAEARGAIAFGAMTWPHQLARIMAAEQAYRALTILAGHPYHRA
                     "
     gene            3229..4584
                     /locus_tag="VKU03_00805"
     CDS             3229..4584
                     /locus_tag="VKU03_00805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLJ69824.1"
                     /translation="MTIRPISARGAAPALAGASAAALALVLFAAPAARGQTPPPSAAA
                     AKPDDQASRQTELRGLEDTMRASEEQRRRIEADVESIKADRARLSAALIETTAKVQEN
                     ERQTNAANQRLATLTDTESAMRKSLENRRGVIADVLAVLQRMGRNPPPAILVKPEDMA
                     QAIRAAILLGAVVPELRAETQALADDLDELAKLRKSIAAQREDLAKRAQGLAADRARL
                     AALIDARQQSLAQAEKALDAERDRAADLARQAATLKDLIAGVENQNAAAKAQAEAARR
                     SNEATAAEIRMKAAALQGKDPTRLKPTLAFADAKGTLELPAAGTIVKAFGAPDAYGGA
                     EKGVSLATPPLATVASPVDGSVVFSGPYRTYGQLLIINAGGGYYVVLAGMDRINVATR
                     QFVLAGEPVATMGDGAQRTATAAAIGAAQPILYIEFRKDGAAIDPGPWWAKADIEKAR
                     G"
BASE COUNT          789 a         1576 c         1498 g          803 t
ORIGIN      
        1 ctaggaagcg ccaatgctga ttgtggggcg gaagcacgaa cccgccgcca tgcaggtaca
       61 aaacctgcgc gcccgcgacg cggcgcgggg tgagcgtgac gatttccttt gtccggatgc
      121 tgacatagga cactgcgaca cgacggcgga ttccgcgaaa atcgcgttcc gacggcgccg
      181 ctttcgcggc ataggcgcgc agcccctccg gcgcgctcag gcgccccttg aagtcgagag
      241 cgcgaagcgc gccggcgacc aaccttgatt ggaaacttgg cagctttggc tccttcgggc
      301 cctgtcgccg cctcggcggc atccgcccaa gcgcttaaac aagttttggg gctgcttcca
      361 tcactctatg tccctcaaca atttggagat ttggcggaaa cggcctggcg cgcagagcga
      421 cgattttttt ctttctgaaa gtcagaatat ctgctaggct tgccgtcttc cgcgagcgtt
      481 caacgcggaa aggcgactcg ttcgcgacga atgacccggt cagacaaaaa acgaagctgc
      541 aatttttttt cattgcaact cattgatctt gttcgtttgg gccaaggaaa gttttggaaa
      601 actttggcgt ggcggatgag ccagcgctga aaagattggg attttttaag ccagatttgg
      661 cgaactcccg ccgcgaaaag ccttttccag gaactttcca aatgggcaag gcgagcgccg
      721 agcgtccgga ccatgtcgag gcccttccgt ccgccgcgaa atgcgctatg cgtccccgct
      781 tcgctgaccc ttgcctccgg ccatgaacga gaaaaaatcc aagccgcgcc gcctcgcctt
      841 cggcgtcgat ccgaagcgcc cgcagcgcta cagcctgcgc caggcgcgat acgacgccct
      901 cgcccatgat atcgacgcgc tcgccgccga ggcggcccgt caggggcggc ggctttcggt
      961 cctcgatatc ggcgccggcg gcggcgcctc gccgctccat ttgcgcgccc gcccgaattt
     1021 cgcgacgatc gatctcgacg gcgccgaccg cgaccggcaa tacaatgtcg acgaatcgct
     1081 ctaccggcgc ttcttcatcg ccgatctgac cgaaggctgt ccggagcttt cctccgaatc
     1141 gtacgacgtc gtgatttgcg agcaggtgct cgaacatatc catgagctgc gcaagccgat
     1201 ggagacgatc ggccgcctgg tcaagccggg cggcaaggcc tttgtcggcg tgccgatctt
     1261 cctgccgccg cttcatttgg cgcgccgcca tctcgtgccg cggctcgacc gcctggtccg
     1321 ctcgacgcac cggcgcgatc acgtccaagc tttcaccctc gccagcgtca agggggcgct
     1381 gctcgcctat tccaagctca gcttcgtcga ggcgcgcggc ttccgcatca tttcgggcgg
     1441 gattttgcgg ccgctcgaag agcggcgcgc atggtgggcg ttcaatcgct ggctcggcgc
     1501 ccgcattccc gccgcctgca tcgaagtgca aatcgtcctc gaaaagccgg ctgcaagcca
     1561 aaaggcatga tcgcgcgcct gccccgcttc agccccggcc agcggatcgg cctgctcggc
     1621 ggcagcttca acccgccgca tcaaggccat ctgcttatct cgctttttgc gcttaagcgc
     1681 ttgcgcctgg atcgggtctg gtggctggcg acgccgggca atccgctcaa ggacaatgaa
     1741 ggcctgccga gcctcgcgaa gcgccttgcc gaggccagcg ccgtcgcccg cgatccacgc
     1801 atcgcggtga ccggcttcga ggccgagatc ggcgcccgct tcaccgcgga cacggtcgct
     1861 tttctgagcc ggcatgcgcc gggcgtgagc ttcgtctggc tgatgggggc cgacaatctg
     1921 cgccagttcc atctctggcg gcgctggcgc cggctcgccg aacaggttcc ggtcgcagcg
     1981 atcgcccggc ccggctcgac cttgaccgcc ttggcggcgc cggcggccgg ctatttcgcc
     2041 aaattccgcg tcccggaagc gcgcgccgcc gatttcgccg gaagtcgacc gcctgcgttc
     2101 atctttttgc atggtccgca atctatgctc tcatcgagcg cgattcggcg cgacgaagct
     2161 gtcgcgaacc gggttgaaaa gcgcgccgag accgctcatg ttttatgaag gccgccgcct
     2221 taagggcggc ctgaaggagg gtcaagactc tgactttagc gcagccccaa aatgcggctg
     2281 ttgagccgcg cccgccggct tcccgttccg tagaatccga agccggcttt ctcgcccaga
     2341 cgatcacgac cagtctcgaa gacgccaagg ctgaagacat cgtttcgatc gatctgcgcg
     2401 gcaaaaccac cctcgccgat ctgatggtca tcgcaaccgg gcgctccaac gtccatgtcg
     2461 gagcgatcgc cgaccgcctg ctgaaggcgt tcaaggccgg cggcctcgcc gcgccgcgca
     2521 tcgaaggcct gccgaatggc gattgggtct tgctcgacgc cagtgacgcg atcgtccata
     2581 ttttccgtcc ggaggtccgc aaattctata acctcgagaa aatgtggggc gcggatcgcc
     2641 cgggcgagaa gcggccggcc cgggcgactt agaaaccgca gcatcgtcaa aggcctgcgg
     2701 tcgtgcgctt gctgttgatc gcggtcggcc gtctgaaggc cggcccggag cgcgaatggt
     2761 tcgagcgcta cgccaagcgg ctcgccgaca tggcgcgggc cgtcgggatc gccagcttcg
     2821 aatggcgcga aatcgaggaa ggccgcgcgc ggcgcgccga agagcgccgc gcggaagagg
     2881 cgcgcgcgat tcgagcggct cttcccgccg gcggcctgct cgtcgtctgc gacgagcgcg
     2941 gcaaggcttt gaccagcgct caattcgccg ccgagatcgg cgcggcgcgc gatcgcggcg
     3001 aagtcgccta cgccctggtt ctcggcggcc ccgacgggct cgagccgaag ctgcgcgccg
     3061 aggcgcgcgg cgcgatcgct ttcggcgcca tgacttggcc gcatcagctt gcgcgaatca
     3121 tggccgccga gcaggcctat cgcgcgctga cgattctggc cggccatccc tatcaccgtg
     3181 cgtgaaggcg ccttattggt aagcttttcc cctttaggct acgcgggcat gacgattcgt
     3241 ccgatttccg cgcgcggcgc cgctccggcg ctggccggcg cgtcggcggc cgcgttggcc
     3301 ctcgtccttt tcgccgcgcc agccgcccgc ggccaaacgc cgccgccttc cgcggccgcc
     3361 gcgaaacccg acgaccaggc ctcgcgccag accgaattgc gcggccttga agacacgatg
     3421 cgcgcgtccg aggagcagcg ccggcggatc gaggcggatg tcgaatcgat caaggccgat
     3481 cgcgcccggc ttagcgccgc cctgatcgag acgaccgcca aggtccagga gaacgagcgc
     3541 cagaccaacg ccgccaatca gcgcctggcg acgctaaccg acaccgaaag cgcgatgcgc
     3601 aaatcgctgg agaatcgccg cggcgtcatc gccgacgtgc tggccgttct ccagcgcatg
     3661 ggccgcaacc cgccgccggc gattctggtc aagcccgagg acatggcgca ggcgattcgg
     3721 gcggccatcc tgctcggcgc ggtcgtgcct gaactcaggg ccgaaaccca ggcgttggcc
     3781 gatgatctcg acgaattggc caagctgcgc aagtcgatcg ccgcccagcg cgaggacctc
     3841 gccaaaaggg cgcaaggcct ggccgccgac cgcgcccggc tcgccgcgct gatcgacgcg
     3901 cgccagcaat ccctggcgca agccgagaag gcgctcgacg ccgagcgcga ccgcgccgcc
     3961 gacctcgccc gccaggccgc cactctcaaa gatttgatcg ccggcgttga gaatcaaaac
     4021 gcggcggcca aggctcaggc cgaagcggcg aggcggagca acgaagcgac ggcggccgaa
     4081 atcaggatga aggcggcggc gctgcaaggc aaggacccga cgcggctgaa accgactctc
     4141 gccttcgccg acgcgaaagg gacgcttgag cttcccgccg ccggcacgat cgtcaaagcg
     4201 ttcggcgcgc cagacgctta tggcggcgcg gagaagggcg tgtcgctggc cacgccgccg
     4261 ctcgccaccg tcgcctcgcc ggtcgacggt tcggtcgttt tttcgggtcc ctaccgcact
     4321 tatggccaac tcttgatcat caatgccggc ggcggctatt atgtcgtctt ggcagggatg
     4381 gatcgtatca acgtcgcgac gcggcagttt gtgctggccg gagagcctgt cgcgacgatg
     4441 ggcgacggcg ctcagcgtac ggcgacggcg gccgccattg gcgccgcgca gccgatcctt
     4501 tatatcgaat tcagaaaaga cggggcggcg atcgatcctg ggccgtggtg ggccaaggct
     4561 gatattgaaa aggcgcgcgg atgatgcgga aggtttccta cttggttctt ggcgctgcga
     4621 ttggcgccgc ggctgttacg gtcggctcac atacgtcttt gcttgc
//
LOCUS       DATGJW010000025         8720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_417328, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000025 DATGJW010000000
VERSION     DATGJW010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8720
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_417328"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(391..1140)
                     /locus_tag="VKU03_00810"
     CDS             complement(391..1140)
                     /locus_tag="VKU03_00810"
                     /EC_number="2.6.99.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033856 - pyridoxine 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxine 5'-phosphate synthase"
                     /protein_id="HLJ69825.1"
                     /translation="MPAPLRLGVNVDHVATVRNARGGRAPDPVRAALLAIEAGADGIT
                     AHLREDRRHIHDEDMTRLRAAIEKPLNFEMAATEQMLDIALKIKPHACCLVPERRTER
                     TTEGGLDVIGGHNHLKPFVSELVRAGIRVSMFIEPSGEALAASASLGAPVVELHTGAW
                     CEALVRRDSEKAAEEFERLKQAAAATRGLKLECHAGHGLDFDSARMIAALPEIVELNI
                     GHFLVGEAIFIGLAESVRTMRAAMDEGRKNA"
     gene            complement(1184..1792)
                     /gene="pyrE"
                     /locus_tag="VKU03_00815"
     CDS             complement(1184..1792)
                     /gene="pyrE"
                     /locus_tag="VKU03_00815"
                     /EC_number="2.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004588 - orotate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotate phosphoribosyltransferase"
                     /protein_id="HLJ69826.1"
                     /translation="MAESDILSESQVLDEFRAAGALLEGHFILSSGLRSPVFLQKMFV
                     FQDPARTERLCRALARKAEAAFGKIDVVVSPALGGIVPGYETARWLGAKAIFVERVDG
                     VFQLRRGFAIEKGARVLMVEDIITTGLSSRECLEAIKAHPGSVVGAACLIDRSGGKAD
                     IGVNRVALATLDIPTYPADALPPELARLPPVKPGSRQSVGAA"
     gene            1892..2284
                     /locus_tag="VKU03_00820"
     CDS             1892..2284
                     /locus_tag="VKU03_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLJ69827.1"
                     /translation="MRDFETLALPPEPSVVAPDGTLGRILLALKGGSLAHFTLPAGAT
                     SHAVKHRTVEEIWFFLSGRGEIWRAGDAREEIVPALPGTSLTIPLGTAFQFRALGGEP
                     LVFLAITMPPWPGDDEAFRVDGPWKAKL"
     gene            complement(2350..2523)
                     /locus_tag="VKU03_00825"
     CDS             complement(2350..2523)
                     /locus_tag="VKU03_00825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69828.1"
                     /translation="MNKEFENPVPPRTKDAGGRIIEDKAAEARERIGRIEKAASGAAA
                     EGSSRRAVIGATL"
     gene            2673..2748
                     /locus_tag="VKU03_00830"
     tRNA            2673..2748
                     /locus_tag="VKU03_00830"
                     /product="tRNA-Thr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2706..2708,aa:Thr,seq:tgt)
     gene            2771..3418
                     /locus_tag="VKU03_00835"
     CDS             2771..3418
                     /locus_tag="VKU03_00835"
                     /inference="COORDINATES: protein motif:HMM:NF042734.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta family hydrolase"
                     /protein_id="HLJ69829.1"
                     /translation="MNASETSSPFQRPGIRGFLHRPAQPSGEGLVLAHGAGANCNAPV
                     LLAAAGAFQAAGVTVLRCDLPFRQKRSSGPPHPSGAAEDREGLRLAAAALREIAAGAV
                     YLGGHSYGGRQASILAAERPDAAEALLLLSYPLHPPNKPEQLRTAHFPNLRAPATFVH
                     GAADPFGSIAEMEQAIASIPAQARLIVADGAGHDLKRGRLDFAPIVAAVQASVRP"
     gene            complement(3422..4918)
                     /locus_tag="VKU03_00840"
     CDS             complement(3422..4918)
                     /locus_tag="VKU03_00840"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLJ69830.1"
                     /translation="MSAYVIGLDIGTTSTIGVLLALPNTIVAVASRKTTLRSPHPGWA
                     EEDPAEWWDNACAVLRELAAALPGERGALRGLCVTGMVPALVLLDEDGQVLRPSIQQS
                     DGRAAGEVEELKREIDEAAFLARTGNGVNQQLIATKLRWLERHEPEVFAKIRTVFGSY
                     DYVNWRLTGMRGVEQNWALEAGIVDLSDHWIKDDLVALTHAPRGAFPAKHASHSVLGA
                     VNASASAATGLPQGLPVFGGAADHIASALAAGLVEPGDVLLKFGGAGDIVVSSDVARP
                     DPRLFLDYHLVPGLYAPNGCMAASGSALNWLAANLRIDEPGGNPHAALDRLAASVAPG
                     GDGVLCLPYFLGEKTPIQDPLARGAFIGLSLSHGPAHLWRALLEAVGFGFRHHVEVLG
                     EIGFAPKRFLASDGGARSRVWMQIVADILQAPVRLVDNAHGSAVGAALVAGVASGGGI
                     GWGDVGRLARLGETIVPQSANGGAYDRSYAKYRALYPALKPILRMDED"
     gene            complement(4938..5738)
                     /locus_tag="VKU03_00845"
     CDS             complement(4938..5738)
                     /locus_tag="VKU03_00845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184646.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HLJ69831.1"
                     /translation="MPFEFFGSRRKVVIAMAHIGALPGTPLYDADGGVPRLIDNVARD
                     IANLQNGGVDAIMFGNENDRPYRFAAPPEGVAAMTAVIAALKPTLKVPFGVNYLWDPV
                     ATVAIACATGASFAREIFTGLFASDMGLWRPNAADALTLRRNLGRPDLKLFYNINAEF
                     AWSLDARPIELRAKSAVFSSLADAILVSGPLTGQAADNSDLKKVREAVPETPVLANTG
                     VRIDNVREVFAIADGCVIGTHFKIDGDTWKPVDGERVKRFMDFVGKLA"
     gene            complement(5762..6595)
                     /locus_tag="VKU03_00850"
     CDS             complement(5762..6595)
                     /locus_tag="VKU03_00850"
                     /inference="COORDINATES: protein motif:HMM:NF012669.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DeoR/GlpR family DNA-binding transcription
                     regulator"
                     /protein_id="HLJ69832.1"
                     /translation="MTEPLLPDLALRRLPAGARHARILELVDRHGFVSVSDIAESFSV
                     SDMTVRRDLGALEEKGMVVRTHGGAVGVGRREVFDAAEPAFSSRRRQNAAAKAAIARA
                     AAKLIAPRESIGLDVGTSTLALAEEIAARRDAMVFTSNLYAAAVLGRGGCPVHVLGGL
                     VRGPELSVVGSNPREQIRQFYVDKLFLGVSGVIEDGFFDYSPEDTEIKRAFIEQAGEI
                     VVLCDASKFGHRSVSKICALDRAAILVTERAPPPHLAEALSRAKVELIVCDFAQSSAL
                     A"
     gene            6709..7491
                     /locus_tag="VKU03_00855"
     CDS             6709..7491
                     /locus_tag="VKU03_00855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183582.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ69833.1"
                     /translation="MAGIFDLTGRRVMVTGGARGIGGAIVRAFARAGARVAIADADLP
                     GAQALAGEIGDAAAAFAIDVRDRTSVEGAFAKIVAHFGGLDILAANAGVSTMQRAVDL
                     TDEEWDFNFAVNARGVFLTNQIACRHFLAEKRAGIIVNTASLAGKVGAPLLAHYSASK
                     FAALGWTQALAREMAPHGIRVNAVCPGFVRTSMQEREIVWESQLRGMTPEAVRTEYVS
                     LTPMGRIEEPEDVADVVLFLASDAARFMTGQGINVTGGVRMD"
     gene            complement(7618..>8720)
                     /locus_tag="VKU03_00860"
     CDS             complement(7618..>8720)
                     /locus_tag="VKU03_00860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLJ69834.1"
                     /translation="DGAERIDLHGALLVPGFVDGHIHLDKTLLGLPFQPHRRGDTVAE
                     RIAREKELRRELRYPVEERSRRLIEQVVAFGTTALRSHVDIDSDVRLSGLHALLKVRD
                     AARDLVGIQLVAFPQSGIVADPGVKDLLDQAIRDGADLVGGLDPAGIDNDVNGHLDAI
                     FAIAERHGVGLDIHLHDPGPLGCFELRQIAMRAAALGLKGRVAVSHAFALGAVDEAEF
                     GRTAEALAAADVAIMTNGPGPVPMPPVKRLIQAGVRVFAGSDNIRDAWSPYGNGDMLE
                     RAMMIGYRQDFRADSDLELAFDLTTRAAARELGLAEYGLEVGAAADLVAIPAASIPEA
                     VAAHPPRTLVLKRGRVLPLAPRSGERVARRAG"
BASE COUNT         1445 a         2951 c         2961 g         1363 t
ORIGIN      
        1 ccctctcccc gcaaagcggg gagaggtacg gagaggggcc ggtcgcctag cttcgactcc
       61 tgagttctgc cccccgcgcc cctctcctta cctctccccg ttccgcttcg cttcacgggg
      121 agaggggccc caagcgccgc ggcaagactc cagcgaccgg cggcgcgcca tgcgcggcgc
      181 ggcaagctaa ggcgcggcgc cgggggcccc tctccccgca aagcggggag aggtacggag
      241 aggggccggt cgcttagctt cgaatcctga gttctgcccc ccgcgcccct ctccgtacct
      301 ctccccgttc cgcttcgctt cacggggaga ggggccccaa gcgccgcggc aaggacgcga
      361 gcgccgcagg ctggccgatc cctccgcgcc tcacgcattc ttccgcccct cgtccatcgc
      421 ggcgcgcatt gtccgcacgc tctcggcaag gccgataaag atggcttcgc cgaccaggaa
      481 atggccgata ttcaactcga cgatttccgg cagcgccgcg atcatccggg cgctgtcgaa
      541 atcgagccca tggcccgcat gacattcgag cttcagcccg cgcgtcgcgg cggcggcctg
      601 cttgagccgc tcgaattcct ccgccgcctt ttcgctgtcg cgacggacga gcgcttcgca
      661 ccacgcgccg gtatgcaatt cgaccaccgg cgcgccgagc gaagcggagg ccgccaaggc
      721 ctcgcccgag ggctcgatga acatcgagac gcgaatgccg gcgcggacga gttcgctgac
      781 gaacggcttc agatgattat ggccgccgat cacatcgagc ccgccctcgg tggtgcgctc
      841 ggtgcgccgc tccggcacca ggcagcaggc gtgcggcttt atcttcagag cgatgtcgag
      901 catctgctcg gtcgccgcca tttcgaaatt gagcggcttt tcgatcgccg ccctcagccg
      961 cgtcatatcc tcgtcatgga tgtgccgccg gtcctcgcgc aaatgcgcgg tgatcccgtc
     1021 cgctcctgct tcgatggcga ggagcgccgc ccgaaccgga tcgggcgcgc ggccgcctcg
     1081 cgcattgcgc actgtcgcga catgatcgac attgacgccg agacgaagcg gagcgggcat
     1141 ggcgaaacct ctcttatcgt accgaccaga acctgcgcct cttctaggcg gccccgacgc
     1201 tctgccggct gccgggcttg accggcggca atcgcgcaag ctccggcggc agcgcgtcgg
     1261 cgggataggt cggaatgtcg agcgtcgcca gcgcgacgcg gttaacgccg atatcggctt
     1321 tgccgccgga tcgatcgatc aggcaagccg cgccgaccac gcttcccgga tgcgccttga
     1381 tcgcctccaa gcactcgcgc gaagagagcc cggtggtgat gatgtcctcg accatcagca
     1441 cccgcgcgcc cttctcgatg gcgaagccgc gccgcaactg gaagacgccg tcgacccgct
     1501 cgacgaaaat cgccttggcg ccgagccagc gcgccgtctc gtagccaggc acgatgcccc
     1561 ccaaagccgg cgaaacgacc acgtcgatct tgccgaaagc ggcctcggct ttgcgcgcca
     1621 aggcgcggca gaggcgctcg gtgcgggccg gatcctggaa gacgaacatc ttctgcagga
     1681 agacgggcga gcgcagtccg gaagacagga tgaaatgccc ttcgagcagg gcccccgccg
     1741 cgcggaactc gtcgagaact tggctttcgc tgagaatgtc ggattcggcc atagcctcaa
     1801 atagcagagg gcgcgccgcg gccaaagcgc gggcggcgct tgcccgggcg cggcggcttc
     1861 gcgcaggata gcggccgcct ggagcccgcc gatgcgcgat ttcgaaaccc tcgctttgcc
     1921 gcccgagccg tccgtcgtcg cgcccgacgg gacgctgggg cgcatccttc tcgcgctgaa
     1981 gggcggcagc ctggcccatt tcaccttgcc ggccggcgcg acctctcacg cggtcaagca
     2041 ccggaccgtc gaggaaatct ggttcttctt gagcgggcgc ggcgaaatct ggcgcgccgg
     2101 cgacgcgcgc gaggaaatcg tccccgccct gcccggaacg tcgctcacca tcccgctcgg
     2161 aaccgccttc cagttccgcg ccctcggcgg ggagccgctg gtcttcctcg caatcacgat
     2221 gccgccctgg ccgggcgacg atgaagcgtt ccgggtcgac gggccttgga aggcgaagct
     2281 gtaggcgcgg ttgttgaccg cgccgctgca atggcgaaaa cccgccccgg ccgcggtcag
     2341 cgaccgcggc tacagggtcg cgccgattac ggcgcgccgg ctcgacccct cggccgcggc
     2401 gccgctcgcg gccttttcga tccggccgat ccgctcgcgc gcctcggcgg ccttgtcctc
     2461 gatgatcctg ccgccggcgt ccttcgtgcg cggcgggact ggattttcaa actccttgtt
     2521 catggcggcc tcgctgatgg gactcaaaag tcacgaccag cggcggccgg aattgttccg
     2581 gccgcgtcca cggccggcga cctgggctct tttcagccgg cctcggcccg cgtatggaga
     2641 gtttccgcgc agagcctcgc caagcccagc atgccggctt agctcagttg gtagagcaac
     2701 tgatttgtaa tcagtaggtc gcgggttcga tccctgcagc cggcaccaca acaaggccac
     2761 tcccgggaaa atgaacgcgt ccgagacctc ctcgcctttt caaaggcccg gcatacgcgg
     2821 cttcctgcat cggccggcgc agccttcggg cgaagggctg gtcttggcgc atggcgccgg
     2881 cgcgaattgc aacgcgccgg ttctgctcgc ggccgcgggc gcgtttcaag cggccggcgt
     2941 gacggtgctg cgctgcgacc tgcctttccg ccagaagcgc agttccgggc cgccgcatcc
     3001 ctccggcgcg gcggaagacc gcgagggctt gcgcttggcc gccgccgcgc ttcgggagat
     3061 cgccgccggc gctgtctatc tcggcggcca ttcctatggc ggccgccagg cgagcatctt
     3121 ggccgcggag cggccggatg cggctgaggc gctgctgctc ctttcctatc cgcttcatcc
     3181 gccgaacaag ccggagcaat tgcgcaccgc gcatttcccc aacctgcgcg cgccggcgac
     3241 cttcgtccat ggcgccgccg atccgttcgg ctcgattgcg gaaatggagc aggcgatcgc
     3301 gtcgattccc gcccaggctc ggctgatcgt cgccgacggc gcgggacacg acctcaagcg
     3361 cggccgtctg gatttcgcgc cgatcgtggc ggctgtccaa gcgagcgttc ggccttagct
     3421 ttcaatcttc atccatccgc aagatcggct tcaacgcagg atagagcgcg cggtatttcg
     3481 cgtaggagcg gtcgtacgcg ccgccattcg ccgattgcgg gacgatcgtc tcgccgagcc
     3541 gggcgaggcg gccgacatcg ccccagccga tcccgccgcc cgaggcgacg ccggcgacga
     3601 gcgcggcgcc aacggccgag ccatgcgcgt tgtccaccag gcgcaccggc gcttgcagaa
     3661 tgtcggcgac gatctgcatc cagacgcggc tgcgcgcgcc gccgtcggaa gcgaggaatc
     3721 gtttcggcgc gaagccgatc tcgccgagca cctcgacatg gtggcggaag ccgaagccga
     3781 ccgcctccaa gagcgcccgc caaagatgcg ccgggccatg gctgaggctg aggccgataa
     3841 aggcgccgcg cgccagcggg tcctggatcg gcgtcttctc gccgagaaaa taaggcaggc
     3901 agagtacgcc gtcgccgccc ggcgcgaccg aggcggcgag gcggtcgagc gcggcgtgcg
     3961 gattgccgcc cggctcgtcg atgcgaagat tggccgcgag ccagttgagc gccgagccgg
     4021 acgccgccat gcagccgttc ggcgcataga ggcctggtac gagatgataa tcgagaaaca
     4081 gccgcggatc gggccgcgca acgtcggacg agacgacgat gtcgccggcg ccgccgaatt
     4141 tcagcagcac gtcgccgggc tcgaccaggc cggcggccag cgccgaggcg atgtggtcgg
     4201 ccgcgccgcc gaagaccggc aggccttgcg gcaatccggt cgccgcagag gcggacgcat
     4261 tcaccgcgcc caagacgcta tgcgaagcat gctttgccgg aaaggcgccg cgaggggcat
     4321 gcgtcagcgc gacgagatcg tcctttatcc agtgatcgga gagatcgacg atcccggctt
     4381 cgagcgccca gttctgctcg acgccgcgca ttccggtcag gcgccagttc acataatcgt
     4441 acgagccgaa aacggtcctg attttcgcga aaacctcagg ctcatgccgc tccagccagc
     4501 gcagcttggt cgcgatgagc tgctgattga cgccgttgcc ggtgcgcgcg agaaacgcgg
     4561 cttcgtcgat ttcgcgcttg agctcttcga cttcgccggc ggcgcgtccg tcgctctgct
     4621 ggatgctcgg ccgcaagacc tgcccgtcct cgtccaacag gacgagagcg ggaaccatgc
     4681 cggtcacgca aaggccgcgg agcgcgccgc gctcgccggg cagcgcggcg gcgagctcgc
     4741 gcagcacggc gcaggcattg tcccaccatt cggccgggtc ctcctccgcc catcctggat
     4801 gcggcgagcg aagcgtcgtc ttgcgcgagg cgacggcgac gatcgtattc ggcaaggcga
     4861 gcagaacgcc gatcgtcgag gtcgtgccga tatcgagacc gatgacgtag gcgctcacat
     4921 tcgcctcgtt tcgccgctca ggccagcttg ccgacaaagt ccatgaagcg cttgacgcgc
     4981 tcgccgtcga ccggcttcca cgtgtcgccg tcgattttga aatgggtgcc gatcacgcag
     5041 ccgtcggcga tggcgaaaac ctcgcggaca ttgtcgatgc gcacgccggt attggcgagg
     5101 acgggcgttt cgggcacggc ctcgcgcacc tttttcagat cggaattgtc cgccgcctgc
     5161 ccggtcagcg gcccggaaac caggatggcg tcggcgagcg aggaaaacac cgcgcttttg
     5221 gcgcgaagct cgatcggccg cgcgtcgagc gaccaggcaa actcggcgtt gatgttgtag
     5281 aaaagcttca aatcgggccg gccgagattg cgccgaagcg tcagcgcgtc ggcggcgttg
     5341 ggccgccaca ggcccatatc ggaggcgaac aggccggtga agatttcgcg ggcgaacgat
     5401 gcgcccgtgg cgcaggcgat ggcgacggtc gcgaccgggt cccaaaggta gttcacgccg
     5461 aacgggactt tcagcgtcgg cttcaacgcg gcgatgaccg cggtcatcgc cgcgacgccc
     5521 tccggcggcg cggcgaaacg gtacggacgg tcattctcgt tgccgaacat gatcgcgtcg
     5581 acgccgccgt tttgcagatt ggcgatgtcg cgcgcgacat tgtcgatgag gcgcggcacg
     5641 ccgccatccg cgtcatagag cggcgttccg ggcagcgcgc cgatatgcgc catggcgatc
     5701 acgactttgc ggcgcgagcc gaagaattcg aaaggcatcg ggagcgtcct tcttgaaaag
     5761 ctcaggcgag cgccgaggat tgggcaaagt cgcagacgat gagctcgacc ttggcgcgcg
     5821 acagcgcctc ggcgagatgg ggcggcggcg cgcgctcggt caccagaatc gcggcgcggt
     5881 ccaaggcgca gattttcgaa acggagcggt ggccgaattt cgacgcatcg cagaggacga
     5941 cgatttcgcc ggcctgctcg ataaaggcgc gcttgatctc ggtatcttcc ggcgaatagt
     6001 cgaaaaagcc gtcctcgatc acgccggaca cgccgaggaa cagcttgtcg acatagaatt
     6061 ggcgaatctg ctcgcgcgga ttcgagccga cgaccgaaag ctccggcccg cgcaccaggc
     6121 cgccaagcac atggacggga cagccccctc gtcccagcac ggccgccgca tagagattgc
     6181 tggtgaacac catcgcgtcg cgccgcgccg cgatctcctc ggcgagcgcc agcgtcgagg
     6241 tgccgacgtc aaggccgatc gattcgcgcg gcgcgatcag cttggcggcg gcgcgggcga
     6301 tcgccgcctt ggccgcggcg ttctggcgcc ggcggctcga aaaagccggc tcggctgcgt
     6361 cgaagacttc gcgccgtccg acccccacgg cgccgccatg ggtgcgtaca accatcccct
     6421 tctcctccaa cgcgccaagg tcgcggcgga cggtcatgtc ggagacggaa aagctctcgg
     6481 cgatatcgga aacggagacg aaaccgtgcc gatcgacgag ttcgagaatg cgggcatggc
     6541 gcgcgccggc cggcagccgc cgcaaggcga gatctggaag caacggctcg gtcacattag
     6601 cgctcatgtg cgattatgat cgtaatcact caaaatcgtc gcgtcgagcg ttaaaatgcg
     6661 ttagattgag cgtcgttgag caaaagtttg aacaggcgga ggggacgcat ggccggaatt
     6721 ttcgatctga ccgggcggcg ggtgatggtg accggcggcg cccgcggcat tggcggggcg
     6781 atcgtccgcg ccttcgctcg cgccggggcg cgcgtcgcca tcgccgatgc ggatcttccc
     6841 ggcgcgcagg cgctggccgg cgagatcggc gacgcggcgg ccgcctttgc gatcgacgtg
     6901 cgcgatcgca catcggtcga gggcgccttc gccaagatcg tcgcccattt cggcggcctc
     6961 gacattctcg ccgctaatgc gggcgtctcg acgatgcagc gcgcggtcga cctcaccgac
     7021 gaggagtggg acttcaattt cgcggtcaat gcgcgcggcg tattcctgac caaccagatc
     7081 gcctgccggc attttctggc cgagaagcgc gccgggatca tcgtcaacac cgcttcgctc
     7141 gccggcaagg tcggcgcgcc gctgctcgcc cattattcgg cgagcaaatt cgccgcgctc
     7201 ggctggacgc aggcgctcgc gcgcgaaatg gcgccgcatg gaataagggt caacgccgtc
     7261 tgtccgggat tcgtgcgcac ctcgatgcag gagcgcgaaa tcgtctggga aagccagctt
     7321 cgcggcatga cgcccgaggc ggtgcgcacc gaatatgtct cgctgacgcc gatgggccgc
     7381 atcgaagagc cggaggacgt ggccgacgtg gtgctttttc tggcgtcgga cgctgcgcgg
     7441 ttcatgacgg gccaggggat caacgtcacc ggcggggtac ggatggattg attgatcccc
     7501 ttctcccgct ccgcggggag aaggtggacg cgcgaagcgc ggccggatga ggggcggcgc
     7561 agaactcggt gggtacgcgc acttcgaagc gctaatccca gaagtcagcg cagcccctca
     7621 cccggccctt cgggccaccc tctccccgct gcgcggggcg agggggagta cgcggccgcg
     7681 cttcagcacc agggtacgcg ggggatgcgc ggcgaccgct tcgggtattg acgcggcggg
     7741 gattgcgaca aggtcggcgg cggcgccgac ttcgagcccg tactcggcga ggccgagctc
     7801 gcgcgccgcc gcgcgggtcg tcagatcgaa ggcaagctcc agatcggaat cggcgcggaa
     7861 gtcctggcgg tagccgatca tcatcgcgcg ttcgagcatg tcgccgtttc catagggcga
     7921 ccaggcgtcg cggatattgt cggagccggc gaagacgcga acgccagcct gaatgagacg
     7981 cttgaccggc ggcatcggca caggacccgg cccgttggtc atgatcgcga catcggcggc
     8041 ggcgagagcc tcggcggtac ggccgaattc cgcctcatcg accgcgccga gcgcgaaagc
     8101 atggctgacc gcgacacggc ctttaaggcc gagcgccgcg gcgcgcatcg cgatctggcg
     8161 caactcgaaa cagccgagcg gccccggatc gtgaagatgg atgtcgaggc caacgccgtg
     8221 acgctcggcg atcgcaaaga tcgcgtcgag atggccgttg acgtcattgt ctatgccggc
     8281 cggatcgagc ccgccgacga gatcggcgcc gtcgcggatc gcctggtcga gcaggtcctt
     8341 gacgccggga tcggcgacga tcccgctttg ggggaaggcg acgagctgaa tcccgacgag
     8401 gtcccgcgcc gcgtcgcgga ctttcagcaa tgcgtgaagg ccggaaaggc ggacatcgct
     8461 gtcgatatcg acatggctgc gcaacgcggt cgtgccgaag gcgacgacct gctcgatcag
     8521 gcggcgcgag cgctcctcga ccggatagcg caattcgcgc cgcagctcct tctcgcgggc
     8581 gatgcgctcg gcgaccgtgt cgccgcgccg atgcggttgg aacggcaagc cgagcagcgt
     8641 cttgtcgaga tgaatatggc cgtcgacaaa gccgggaacg agcagcgcgc catgaagatc
     8701 gatccgctcg gcgccgtcgg
//
LOCUS       DATGJW010000026         3777 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1351108, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000026 DATGJW010000000
VERSION     DATGJW010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3777)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3777)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3777
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1351108"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..410
                     /locus_tag="VKU03_00865"
     CDS             108..410
                     /locus_tag="VKU03_00865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69835.1"
                     /translation="MYEIVTRSNEERLERLIDRLPGRLQAATRWLRRPSSRWARIPAG
                     ALLILGGFLSILPVFGLWMLPLGFVLLADDLPYFRRLREALLDWLERRRPDWFAER"
     gene            490..813
                     /locus_tag="VKU03_00870"
     CDS             490..813
                     /locus_tag="VKU03_00870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011472049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HLJ69836.1"
                     /translation="MIETFAGREKGFEAEFARNEELAFRLTARRNRLFGLWVAERLDL
                     PAAEASAYAAAAASAALDAPGEEDFIRKVLADLTARGLDVREAELRREFARAADTARH
                     EIFGA"
     gene            889..3558
                     /locus_tag="VKU03_00875"
     CDS             889..3558
                     /locus_tag="VKU03_00875"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012905.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HLJ69837.1"
                     /translation="MPTNDAHPAWHTLAAEAAAESLETDLAAGLPPHEASLRLAQYGP
                     NELIEQGRRNFFRIALAQFSDFMIVVLIAAALISWLVGDSEDAIVIFAIVLLNGAIGF
                     AQEIRAERAIAALKQLAALTAEVVRAGRRMTLAAAEIVPGDLVRVEAGNAVPADLRLV
                     ESSALKINESSFTGESVPVEKQAGPLPQRDAPLAERVNMAFKGTVATYGRGLGLAVAT
                     GMATELGKIAGMLAAAPAEPTPLQKRLAAFGRQLGFAVLAVCAVVFAMGLWRGEPILL
                     MLLTAISLAVAAIPEALPAVVTVMLATAARTMARRQALVRRLPAVETLGSVSCICTDK
                     TGTLTLNEMRARQAYIRDAYIEAAALDPSRPSARRFLAALALCNDAARGEERVAGDPT
                     EIALWRLAADAGFDKAALERSHPRVLELPFDSERKRMTTIHRAEDGFVAFTKGAPESV
                     LGRCVKSAGDEADGALAGDKLLAAAEAMAGRGLRVLAIAERRWETPPEELDVDFVERD
                     LTLLGLIGLVDPPRPEAKNAVALCKSAGIVPVMITGDHPATARAIAVALGITGPDDPV
                     LTGEALARLGEPELADRIKDTRVYARVDPAQKIRIVKAARASGQFVAMTGDGVNDAPA
                     LAYADIGVAMGKTGTDVAREAASLILLDDNFATIVAAVAEGRRIFDNVRKFVSYVLTC
                     NAAEIWTILLAPFFGLPLPLLPIHILWINLVTDGLPGLALAAEPAEAKAMQRPPRPAN
                     EGIFAHGMWRHILWIGLAMSAVTLGTQAYAIRVAGLAHWQTMVFTVLTLSQMGAALAT
                     RSERESLFRQGLFSNRYLVGAVFLTMALQLLLIYVPFFNDVFKTAPLSAAELALCLVV
                     SSVVFFVVEAEKWLARRGLLRRGRPELLSQSAA"
BASE COUNT          572 a         1289 c         1248 g          668 t
ORIGIN      
        1 cgcgagcaga aaatgctcgc cttgatccgg cgcgtcttcg gcggcgcgcc gctgcccttc
       61 gtccgcggcg atttgcaacg agcgccgaac tgaaagtgaa gagcgcaatg tacgagatcg
      121 tgacgcgttc gaacgaagag cggcttgagc gattgatcga ccgcttgccc ggccggctgc
      181 aagccgcgac gcgctggctg cgccgcccgt cctcgcgctg ggcgcggatt cccgcgggcg
      241 ccttgctgat ccttggcggc tttttgagca tccttccggt gttcgggctt tggatgctgc
      301 cgctcggctt cgtcctgctc gcggacgacc ttccgtattt ccggcgcttg cgcgaagcgc
      361 tgctcgattg gctcgaacgg cgccggccgg attggttcgc cgagcgctaa ttgcgtcgag
      421 cgccgcacaa agacgacggc gcgaggagag aagaccgcct cgcctttcac attcttaagg
      481 agaacgaaca tgatcgagac tttcgccggg cgtgaaaaag gattcgaagc ggagtttgcc
      541 cgcaacgagg agctggcgtt tcggcttacg gcgcgccgga accggctgtt cgggctttgg
      601 gtcgccgagc gcctcgacct tccggctgcg gaagcgtcgg cctacgccgc cgcggccgcc
      661 tcggcggcgt tggatgcgcc gggcgaggag gatttcatcc gcaaggtcct cgccgacttg
      721 accgctcgcg gcctcgatgt tcgcgaagcc gagttgcgtc gggaattcgc tcgcgccgcc
      781 gacacggctc gccacgagat ttttggcgca taggcaaagc catccgcgcc ggccaaccta
      841 tcttagccgg cgtcgttccg acgccggcgc cccaatttag acatttgcat gccgacgaat
      901 gacgctcatc ctgcctggca tacgctcgct gccgaggcgg ccgccgagtc tttggagacc
      961 gatctggccg cgggcctgcc gccgcacgag gcgtccctgc ggctcgctca atatggcccc
     1021 aacgagctga tcgagcaggg gcggcgcaat ttcttccgga tcgcgctcgc gcagttcagc
     1081 gatttcatga tcgtcgtcct gatcgccgcg gcgctgattt cgtggctggt cggcgattct
     1141 gaggacgcga tcgtcatctt cgccatcgtc ctgttgaacg gcgcgatcgg ctttgcgcag
     1201 gagattcgcg ccgagcgcgc catagcggcg ctgaagcagc ttgccgccct caccgccgag
     1261 gtcgtccgcg ccggacgccg catgacgctc gccgccgccg agatcgtacc cggcgatctc
     1321 gtcagggtgg aggccggcaa tgcggtgccg gccgacctgc ggctggtcga atcctcggcg
     1381 ctcaaaatca acgaatcgtc cttcaccggc gaatccgtcc ccgtcgagaa acaggccggc
     1441 ccgctgccgc agcgcgacgc gcctttggcc gagcgggtca acatggcgtt caaaggtacg
     1501 gtcgcgacct atggccgcgg cctcggcctc gccgtcgcga ccggcatggc gaccgagctc
     1561 ggcaagatcg cgggcatgct ggctgccgcc ccggccgagc cgaccccgct tcagaagcgg
     1621 ctcgccgctt tcggccggca attgggattc gccgtcctcg cggtctgcgc cgtcgttttc
     1681 gccatgggcc tgtggcgcgg cgagccgatc ctgctgatgc tgctgacggc gatcagcctc
     1741 gctgtcgcgg ccattcccga agccttgccg gcggtggtga cggtcatgct ggcgaccgcg
     1801 gcgcggacca tggcgcggcg ccaggccctg gtgcgccggc tgccggccgt cgaaacgctc
     1861 ggctcggtga gctgcatctg caccgacaag accggcacgc tgacgctgaa cgagatgcgc
     1921 gcgcgccagg cctacatccg cgacgcgtat atcgaggcgg cggcgctcga cccgtcgcgg
     1981 ccgtccgcac ggcgcttcct cgccgcgctc gcgctgtgca acgatgcggc gcgcggggag
     2041 gagcgcgtcg cgggcgatcc gaccgagatc gcgctctggc gcctcgccgc cgacgccggc
     2101 ttcgacaaag ccgcgctcga gcgctcgcat ccgcgggttc tcgagcttcc gttcgattcc
     2161 gagcgcaagc ggatgacgac gatccaccgc gccgaggacg gcttcgtcgc ctttaccaag
     2221 ggggcgcccg aatcggttct cggtcgctgc gtcaaatcgg ccggcgacga agcggacggc
     2281 gcgctcgccg gggacaagct gctcgccgcg gccgaggcga tggcgggcag gggcttgcgc
     2341 gtgctcgcca tcgcggagcg gcgttgggag acgccgccag aagagctgga tgtcgacttt
     2401 gtcgagcgcg atctgacctt gctcgggctg atcggccttg tcgacccgcc gcgcccggaa
     2461 gccaaaaacg ccgtcgcctt gtgcaagtcg gccgggatcg tcccggtcat gatcaccggc
     2521 gatcatccgg cgacggcgcg cgccatcgcc gtcgccctcg gcataaccgg gccggacgat
     2581 cccgtcctga ccggagaggc tctcgcccgc ctcggcgagc cggagcttgc cgaccgcatc
     2641 aaggacacgc gcgtctacgc ccgcgtcgac ccggcgcaga aaatccgcat cgtcaaggcg
     2701 gcgcgcgctt ccggccaatt cgtcgcgatg accggagacg gcgtcaacga cgcgccggcg
     2761 ctcgcctatg ccgatatcgg cgtcgccatg ggcaagacag gcaccgacgt cgcccgcgag
     2821 gcggcgagcc tcattctgct cgacgacaat ttcgcgacga tcgtcgcggc cgtcgccgaa
     2881 ggtcggcgca ttttcgacaa tgtccggaaa ttcgtcagct atgttctgac ctgcaacgcc
     2941 gcggagatct ggacgatcct gctggcgccg tttttcggcc tgccgcttcc gctgctgccc
     3001 atccacattc tctggatcaa cctggtcacc gacggcctcc ccggcttggc gctcgccgcc
     3061 gagccggccg aggcgaaggc gatgcagcgt ccgccgagac cggcgaacga aggcattttc
     3121 gcccacggca tgtggcggca cattttatgg atcggattgg cgatgagcgc ggtgacgctt
     3181 ggaacccagg cttatgcgat ccgcgtcgcc ggcctggccc attggcaaac gatggtcttc
     3241 accgtcctga cgctttcgca gatgggcgcc gcgctcgcca cgcgctccga gcgggaatcg
     3301 ctattcaggc aaggcctgtt ctcgaaccgc tatctggtcg gcgccgtgtt cctgacgatg
     3361 gcgctgcagc ttctgctgat ctacgtcccc ttcttcaacg acgtgttcaa gacggcgccg
     3421 ctctcggcgg cggagctggc gctgtgcctc gtcgtctcaa gcgtcgtttt cttcgtcgtc
     3481 gaagccgaaa agtggctggc gcgccgcggc ctgcttcgcc gaggacgccc ggaactattg
     3541 tcgcagtcgg ccgcctagtc ctcctgacgc cgatggatcg gtttgcgctt accctctccc
     3601 gcgtgcgtcc ccacgacgga gggataagaa ggcgaccgtc agatgtccga ggtcgaagat
     3661 gtgttccgca tgtcatagac attgaagctg agacctgacg ggccgaagag cctcataaag
     3721 tcccgcgtat cataggtggc aatttgaaca gccggccgcg tatcacactc gcgagat
//
LOCUS       DATGJW010000027         2688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1456226, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000027 DATGJW010000000
VERSION     DATGJW010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2688
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1456226"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..642)
                     /locus_tag="VKU03_00880"
     CDS             complement(<1..642)
                     /locus_tag="VKU03_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLJ69838.1"
                     /translation="MLGLMQDWPLLLHKIIDFAAIQHGEREVVSRTVEGPMHRETYRQ
                     VRERALKGAQRLARDGIKLGDRVATLAWNSHRHLESWYSIVGLGAIYHTVNPRLFPDQ
                     IAWIVNHAEDRMVMVDLTFVPLLEALADKLPTVERYIVFTDAAHMPQTRLKNAVPYEE
                     WLAEADGDFRWADFDEQTAAGLCYTSGTTGNPKGVLYSHRSNVLHSLACTGDDG"
     gene            807..1259
                     /locus_tag="VKU03_00885"
     CDS             807..1259
                     /locus_tag="VKU03_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ69839.1"
                     /translation="MSEEAGEAEAVRVWFRLMRLETRMRVAVSDRLRHLGLSVPQCDV
                     LTTLTEREGISQQALAQRLYVTKGNISGLIDRLVAAGLVERRPIAADKRQHALYLTPA
                     GRAAAEKAIACQRRFVAETIGRLPAGKLSSLDQLLTEARDLVRTRAGG"
     gene            complement(1314..2045)
                     /locus_tag="VKU03_00890"
     CDS             complement(1314..2045)
                     /locus_tag="VKU03_00890"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLJ69840.1"
                     /translation="MPIAAILFDKDGTLIDFDGTWGPATYEVMRALAKGDEAALLRQA
                     EALHFSLDTRRFLITSPLIAGSSASYGSLWGEALGRRDLDALKREIDALTAIESLKSL
                     APIGRPSEVLAALKARGLRLGLATNDSEASARRQAAALGLTELLDFIAGYDSGHGGKP
                     EPGMALAFARHVGVAPGAVAMVGDTLHDLRSARAAGALAVAVLTGPAAIADLADEADH
                     VLDDIGGLLDLIDTLAAAEAAIETG"
     gene            complement(2110..>2688)
                     /gene="yihA"
                     /locus_tag="VKU03_00895"
     CDS             complement(2110..>2688)
                     /gene="yihA"
                     /locus_tag="VKU03_00895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTP-binding protein
                     YihA/YsxC"
                     /protein_id="HLJ69841.1"
                     /translation="IYAASRPDALPPIGQPEIAFAGRSNVGKSSLINALTGRQTLART
                     SHTPGRTQELIFFDLAGALRLVDMPGYGYAAVGKEKAASWGRLIRDYLRGRSSLLRVF
                     LLVDGRHGFKDKDGEMMQALDEAAVSYAVVLTKADAVKAAERAARLESAEAALKRRPA
                     AYPEALFVSAHKGDGVADLRAHIARLLAERGG"
BASE COUNT          425 a          893 c          959 g          411 t
ORIGIN      
        1 gccatcgtcg ccggtgcagg cgagcgaatg caaaacgttg gagcggtgcg aatagagcac
       61 gcccttggga ttgccggtcg tgcccgacgt gtagcaaagg ccggcggcgg tttgctcgtc
      121 gaaatcggcc cagcggaaat cgccgtccgc ttccgccagc cattcctcgt aggggacggc
      181 gttcttcaat ctcgtctgcg gcatatgcgc ggcgtcggtg aagacgatgt agcgctcgac
      241 ggtcggcagc ttgtcggcca gggcttcgag cagcggcacg aaggtcaggt cgaccataac
      301 catccggtcc tcggcgtgat tgacgatcca ggcgatctga tcgggaaaga ggcgcggatt
      361 gacggtgtgg tagatcgcgc cgaggccgac gatcgagtac caggattcga gatggcgatg
      421 cgagttccag gcgagcgtcg cgacccggtc gccgagcttg atcccgtcgc gcgccaggcg
      481 ctgcgcgcct ttcagggcgc gctcgcgcac ctggcggtag gtctcgcgat gcatcgggcc
      541 ttcgacggtc cgcgagacga cctcccgctc gccatgctgg atcgccgcaa agtcgatgat
      601 tttgtgcagc agcagcggcc aatcctgcat cagtcccagc atgtcgtcct ccctcccggc
      661 ggcgggcgtt gagcatccgc ggcgcgccgg aaattattac tcttatcgta acatgacggg
      721 cttggccgcg catccaaatt gcccgttggc gaataggcca ttagcatagg ttcggacgct
      781 cggcgtcggg tcacgaggcg aggcggatga gcgaggaggc gggcgaagcg gaagcggtgc
      841 gggtatggtt ccgcttgatg cgcctggaga cacgcatgcg cgtcgcggtc tccgaccggc
      901 tgcgccatct cgggctctcc gtgccgcaat gcgacgtgct gacgacgctg accgagcgcg
      961 agggaatcag ccagcaggcg ctggcccaac ggctctatgt gaccaagggc aatatttccg
     1021 gcctgatcga ccgcctggtc gccgccggcc tggtcgagcg ccggccgatc gccgccgaca
     1081 agcgccagca tgcgctctat ctcaccccgg ctggccgcgc cgccgccgaa aaggcgatcg
     1141 cctgccagcg ccgcttcgtc gccgagacga tcggccgcct gcccgccggc aaactgagct
     1201 cgctcgatca actcctgaca gaggcgcgcg acctggtgcg cacgcgggcg ggcgggtgag
     1261 gaggagacgg gccgtggccg ccattgcgag gagcgacgca atgacggaag ggcttagccc
     1321 gtctcgatcg cggcttccgc ggcggcgagc gtgtcgatca agtcgagcag cccgccaatg
     1381 tcgtcgagca catgatcggc ttcgtcggcg aggtcggcga tggcggcggg gccggtgagg
     1441 accgcgacgg cgagcgcgcc ggcggcccgg gccgagcgca ggtcgtgcag cgtgtcgccg
     1501 accatcgcca ccgcgcccgg cgcgacgccg acatgccggg cgaaagcgag cgccatgccc
     1561 ggctccggct tgccgccatg gccggaatcg tagccggcga tgaaatccag gagttcggtc
     1621 aggcccaatg cggccgcctg ccgccgcgcg ctggcctccg aatcgttggt cgccaggccg
     1681 agccgcaagc cgcgcgcctt cagcgcggcg aggacttcgc tcggccggcc gatcggggcc
     1741 aagcttttca gcgattcgat cgcggtgagc gcgtcgatct cgcgctttag cgcgtcgagg
     1801 tcgcggcggc cgagcgcctc gccccagagc gagccatagc tcgccgagga gccggcgatc
     1861 aggggcgaag tgatcaggaa ccggcgcgtg tcgagcgaaa aatgcagggc ctcggcctga
     1921 cgcagcagcg ccgcctcgtc gcccttggcg agggcgcgca tgacctcgta ggtcgccggc
     1981 ccccaggtcc cgtcgaaatc gatcagggtc ccgtccttat cgaacaggat ggcggcgatc
     2041 ggcatgggac ctcttctttt ttcgcgtgaa cgcgccggca atagcaaatg cgggcgcgcg
     2101 ccgtcgactc tacccgccgc gctcggcgag aaggcgcgcg atatgggcgc gcagatcagc
     2161 gacgccgtcg cccttatgcg ccgagacgaa cagcgcctcg ggataggcgg cggggcgccg
     2221 cttcagcgcc gcctcggcgc tttccagccg ggcggcgcgc tcggccgcct tgaccgcgtc
     2281 ggccttggtc aggaccacgg cgtaggagac ggccgcctcg tcgagcgcct gcatcatctc
     2341 gccgtccttg tccttgaagc cgtggcggcc gtcgaccagc aggaagacgc gcagcagcga
     2401 cgagcggccg cgcagatagt cgcggatcag cctgccccag gaggccgcct tctccttgcc
     2461 gactgcggca tagccgtagc cgggcatgtc gacgagcctc agcgcgccgg cgaggtcgaa
     2521 gaaaatcagc tcctgggtgc ggccgggcgt gtgcgaggtc cgcgccaggg tctgccggcc
     2581 ggtcagggcg ttgatcagcg acgatttgcc gacattggag cggccggcaa aagcgatttc
     2641 cggctgcccg atcggcggca gcgcgtccgg ccgcgacgcg gcgtagat
//
LOCUS       DATGJW010000028         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1145411, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000028 DATGJW010000000
VERSION     DATGJW010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1145411"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..925)
                     /gene="dnaN"
                     /locus_tag="VKU03_00900"
     CDS             complement(<1..925)
                     /gene="dnaN"
                     /locus_tag="VKU03_00900"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit beta"
                     /protein_id="HLJ69842.1"
                     /translation="MKATLERTALLKALGHVHRIVERRNTIPILSNVLIEARERQLIL
                     KATDLDLEATESAPADIAQDGSTTVAAHVLYDIVRKLPEGAQVSLDMTGETGQLLLRS
                     GRSRFHLQSLPASDFPDLATGELSHRFTLAAADLKKLIDNTQFAISTEETRYYLNGIF
                     IHTVDVSGATMLRAVATDGHRLARVEMPAPQGAAGMPGVIVPRKAVSEIQKLIEDIDA
                     EVSIELSTAKARFRFGETVLTTKLIDGTFPDYARVIPAGNDKRLVVDKAPFQAAVDRV
                     STISSERGRAIKLSIADGKMTLSVNNPDSGSA"
     gene            1178..>2111
                     /locus_tag="VKU03_00905"
     CDS             1178..>2111
                     /locus_tag="VKU03_00905"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HLJ69843.1"
                     /translation="MFGLGRRRNRERIEPRFESSRDRSADLRADPEDRPRRGGGGGKK
                     RAGPARRKERRARRSGFGRLLYWCFVLGVWAFIGVGGLFAYYASQLPPIDQLAVPKRP
                     PNIAILADDGALLANRGDTGGAAVHLSELPPYLPKAFVAIEDRRFYSHFGIDPIGIMR
                     AVARDILHRGGATEGGSTLTQQLAKNLFLTQERTISRKIQEAILALWLERKYSKDQIL
                     ELYLNRVYFGSGAYGVEAAAQKYFGKSARFVTLSEAAVLAGLMKSPTRLAPNHNLDAA
                     NARAAQVITAMAEQGYITEAMAKLALARPAEVVKQ"
BASE COUNT          343 a          710 c          707 g          351 t
ORIGIN      
        1 tggccgagcc ggaatcagga ttgttgacgg agagcgtcat cttgccgtcg gcgatggaga
       61 gcttgatcgc ccggccacgc tccgacgaga tcgtcgagac gcggtcaacc gcggcctgga
      121 acggcgcctt gtcgacgacg aggcgcttgt cgttgccggc cgggatgacc cgggcgtagt
      181 ccggaaaggt cccgtcgatc agcttggtcg tcagcaccgt ctcgccgaag cggaagcgcg
      241 ccttggcggt cgacagttcg atcgagacct cggcgtcgat atcctcgatc agcttctgga
      301 tttcgctcac cgccttgcgc ggcacgatca cgccgggcat gccggccgcg ccctgcgggg
      361 ccggcatttc cacccgggcg aggcggtgac cgtcggtcgc cacggcgcgc agcatggtcg
      421 cgcccgagac gtcgaccgtg tggatgaaaa tgccgttgag gtagtagcgc gtctcctcgg
      481 tcgagatcgc gaactgcgta ttgtcgatga gcttcttgag atcggcggcc gcgagggtga
      541 agcgatggct cagttcgccg gtggcgaggt cgggaaagtc cgaggccggc agcgactgca
      601 agtggaagcg cgagcggccg gagcgcaaga ggagctggcc tgtctcgccg gtcatgtcga
      661 gcgaaacctg cgcgccttcc ggcaacttgc gcacgatatc gtagagcaca tgggcggcga
      721 ccgtggtcga accgtcctgg gcgatatcgg ctggcgccga ttcggtcgcc tccaggtcga
      781 gatcggtcgc tttcaggatg agctggcgct cccgcgcctc gatcagcacg ttcgagagaa
      841 tcggaatcgt gttccgccgc tcgacgatgc gatgcacatg ccccagagct ttgagcaggg
      901 ccgtccgctc caacgtcgct ttcatcgcag cgtcccgaac ctcaaatacg cgcagagccg
      961 gcaatccggc gggccgcgac tgtggcaaga gggccgcgcc ggcgcaaggc cgcgccgccg
     1021 gcttccccag ccgttttgcc tcccggccgc cgcgcgccgg gcgacttaac cggcccttaa
     1081 atcgccgcct ttagcttcga ttgcctggct cggcaagcgc cgcgcgccgg gccttcgatc
     1141 cgggcggcgg ctcgcgggac cttgggggca ggttgacatg ttcggcctcg gacgcaggcg
     1201 aaaccgcgag cggatcgagc cgagattcga atcctcgcgc gaccgcagcg ccgatttgcg
     1261 cgccgatccc gaagatcggc ctcgccgcgg cggcgggggc ggcaagaaac gcgccggccc
     1321 cgctcggcga aaggagcgtc gagccaggcg ctccggattc gggcgcctcc tctattggtg
     1381 cttcgtgctc ggcgtctggg cgtttatcgg cgttggcggc ctcttcgcct attacgccag
     1441 ccaattgccg ccgatcgatc agctggccgt gccgaagcgg ccgcccaaca tcgccattct
     1501 cgccgatgac ggcgcgctgc tcgccaatcg cggcgacacc ggcggcgcgg cggtccatct
     1561 gagcgagttg ccgccctacc tgcccaaggc gttcgtcgcc atcgaggaca ggcgcttcta
     1621 ttcgcatttc ggcatcgatc cgatcggcat catgcgcgcc gtcgcgcgcg acatcctcca
     1681 tcgcggcggc gccacagagg gcggctcgac cctgacccag cagctcgcca agaacctgtt
     1741 cctgacgcag gagcgcacga tctcgcgcaa gatccaagag gcgatcctgg cgctgtggct
     1801 cgagcgcaaa tattcgaagg accagattct agaactctat ctcaaccgcg tctatttcgg
     1861 ctcgggcgcc tatggcgtcg aagcggcggc gcagaaatat ttcggcaaga gcgcccgctt
     1921 cgtgacattg tcggaagccg ccgtgctcgc cggcctgatg aagtcgccga cccggctcgc
     1981 gcccaatcac aatctcgacg ccgccaacgc ccgcgccgcc caggtcatca ccgccatggc
     2041 cgagcagggc tatatcaccg aggcgatggc caagctggcg ctggcgcggc cggccgaagt
     2101 ggtcaagcag a
//
LOCUS       DATGJW010000029         2438 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1248928, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000029 DATGJW010000000
VERSION     DATGJW010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2438)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2438)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2438
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1248928"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2438)
                     /gene="malQ"
                     /locus_tag="VKU03_00910"
     CDS             complement(<1..>2438)
                     /gene="malQ"
                     /locus_tag="VKU03_00910"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HLJ69844.1"
                     /translation="GLNRDAFLTGAPFDASGLPDVEWRDANGPLNEGGWNDPAGAVLV
                     AVFAAPQGDGVDRVAVGMNRSDADTELCLPPPRAGMAWRALIDTHDPDAPERFLAIAD
                     RVRLRARSSLIVAETPASGGLHSGAPTIETIDTLANAAGIAAEWWDVGGKRTLVSAET
                     RIALLEALGLDVASEAQARDSLTRLIDDTGRRRLPFSLVLRQGEPLTAPLRDMPRAAE
                     ARIVGEDGAATEWTIEAAGGAERRLPDGRSVVERAIALPSLPIGRHRLIVDGVECALT
                     IAPSQAYSPEAASRKRFGVAAQLYALRREGDQGIGDFSASGEAGEAAGKAGAAYLGLS
                     PLHMLFPGNRERASPYFPSDRRFLDPIFIDALDDAGLPRDEALNGALAASAPAFAAAS
                     ATAHVEYEPVWRAKRAALEARCAAFSRLRAARPSDPLVADYHAFARQGGETLRRFAAF
                     QATADGKLGENWRAWPQGLRDGEPKAIDAAIERNRQAFEFALFCQWLADRQLGRAAAR
                     ARKGGLEIGFYRDLAVGAAPDGAESWAHAPALARGVTVGAPPDPFSSEGQNWSLPAFN
                     PLAGAREGWASFSAVYRANMRHAGMLRIDHAMGLERLFLIPEGARPAEGAYLAYPFDD
                     LIGHVALESQRAQCMVVGEDLGTVPEGFRDRLTRARIAGMRVLRFERDGARVRAPESY
                     PPMSVACVATHDLATLAGWQQGADIGERLMLGLLTLAKAGEAIAARREEKRGLVAALL
                     AAGLISSAPREDAPLTEAVAVAVHALIGGAGSILAHAQFDDLVGEIVQTNLPGTDQER
                     PNWRLKSSLDVTAA"
BASE COUNT          354 a          882 c          874 g          328 t
ORIGIN      
        1 ggccgccgtg acatccagac tcgacttgag gcgccagttg gggcgctctt ggtccgttcc
       61 gggcaggttg gtctgcacga tttcgccaac cagatcgtcg aactgggcgt gggccaggat
      121 cgagcccgcg ccgccgatca gcgcgtggac cgccacggcg accgcctcgg tgagcggcgc
      181 gtcctccctc ggcgccgagg agataagccc cgcggcgaga agcgccgcga caagcccgcg
      241 cttctcctcc cgccgcgcgg cgattgcctc gcccgccttg gccagggtca gaaggccgag
      301 catgagccgc tcgccgatgt cggcgccttg ctgccagccg gcgagcgtcg ccagatcgtg
      361 cgtcgcgacg caggcgaccg acatcggcgg ataggattcc ggcgcccgca ccctggcgcc
      421 gtcgcgttcg aaccgcagca cccgcatgcc cgcgatcctg gcgcgggtca gccggtcgcg
      481 gaagccctcc ggcacggtgc cgaggtcctc gccgaccacc atgcattgag cgcgttggct
      541 ttcgagcgcg acgtggccaa tcaggtcgtc gaatggatag gcgagatagg cgccttccgc
      601 cggacgggcg ccctcgggga taaggaagag gcgctcgagc cccatcgcgt gatcgatcct
      661 gagcatgccg gcgtgtcgca tgttggcgcg atagacggcg ctgaaggaag cccagccctc
      721 gcgcgcgccg gccagcggat tgaacgccgg caggctccag ttctgccctt cggacgaaaa
      781 cggatcgggc ggcgctccga ccgtgacgcc gcgcgccagc gccggggcgt gggcccagga
      841 ttcagcgccg tctggcgcgg cgccgaccgc aagatcgcga tagaaaccga tctcgagacc
      901 gcccttgcgc gcgcgcgccg ccgcccgccc gagctgccgg tcggcgagcc attggcagaa
      961 cagcgcgaac tcgaaagcct gccggttgcg ctcgatcgcc gcgtcgatcg ccttgggctc
     1021 gccgtcgcgc aagccctgcg gccatgcccg ccagttctcg ccaagcttgc cgtcggcggt
     1081 cgcctggaag gcggcgaagc ggcgcaaggt ttcgccgcct tgtcttgcga aggcgtggta
     1141 gtcggcgacc agcggatcgc tcggcctcgc cgcgcgcagg cgggagaagg cggcgcatcg
     1201 ggcctcgagc gccgcgcgct tggcgcgcca gaccggctcg tactcgacat gagcggtcgc
     1261 cgaagccgcg gcgaaagcgg gcgccgatgc ggcaagcgcc ccgttcagcg cctcgtcgcg
     1321 cggcagcccc gcgtcgtcca gcgcgtcgat aaagatcgga tcgaggaagc ggcgatcgga
     1381 cgggaaatac gggctcgccc gctcccggtt cccggggaac agcatgtgca gcgggctcaa
     1441 ccccagatag gccgcgccgg ccttgcccgc cgcctcgccc gcctcgccgc tcgcggaaaa
     1501 gtcgccgatc ccctggtcgc cttcgcgacg caaggcgtag agttgggcgg cgacgccgaa
     1561 ccgtttgcgc gacgccgcct cagggctgta cgcttgcgac ggcgcgatgg tcagcgcgca
     1621 ttcgaccccg tcgacgatga gacggtggcg cccgatcggt agcgaaggca gcgcgatcgc
     1681 gcgctcgacg accgagcggc cgtcgggcag gcggcgctcg gcgccgccgg cggcttcgat
     1741 tgtccattcg gtcgcggcgc cgtcctcgcc cacgatccgc gcctcggccg cgcgcggcat
     1801 atcgcgcaag ggcgcggtca gcggctcgcc ctggcgcagc acgagcgaga agggaaggcg
     1861 gcggcgcccg gtgtcatcga tgagccgggt caggctgtcg cgcgcctgag cctcgctcgc
     1921 gacatcgagg ccgagcgcct ccagaagcgc gatcctggtc tccgctgaaa ccagagtgcg
     1981 cttgccgccg acgtcccacc attccgccgc aatgcccgct gcattcgcca gcgtgtcgat
     2041 cgtctcgatg gtcggcgcgc cggaatgaag gccgccgctc gccggcgtct cggcgacaat
     2101 gagggaggag cgcgcccgaa gccggacgcg gtcggcgatc gcgagaaagc gctcgggcgc
     2161 gtcgggatca tgggtgtcga tcagcgcgcg ccacgccatg ccggcgcgcg gcggcggcag
     2221 acaaagctct gtgtcggcgt ccgaccggtt catcccgacc gcgacgcggt cgacgccatc
     2281 gccctgcggc gccgcgaata cggcgaccag gacggccccc gcgggatcgt tccacccgcc
     2341 ctcgtttaag ggcccattgg cgtcgcgcca ctcgacgtcg ggaaggccgc tcgcgtcgaa
     2401 cggcgccccg gtcaggaaag cgtcgcggtt gagcccgg
//
LOCUS       DATGJW010000030         2545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1249561, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000030 DATGJW010000000
VERSION     DATGJW010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2545
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249561"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1513)
                     /locus_tag="VKU03_00915"
     CDS             complement(134..1513)
                     /locus_tag="VKU03_00915"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HLJ69845.1"
                     /translation="MLAVEVGFSYVAAAADFGAIVAAAAASHILYRLATFGFFPRAES
                     VVEVGLVLGAVVVALNAQRGEYDIKQYAQMEGHIGRCLSTWNIAFFCVLALGFVTKTT
                     EVFSRGATAVLYVAGFLALGGARASLVALIAAAKKRRLLPRRRLVLVGLETQLASFAE
                     RYDLDRSGMEVVSAAVIRDGDGTVRDDLALAAATVRVLRPDDVFIAIPWARARLIETC
                     VDAFIRTPAEIHLGPEEILDRFTDAEVAKLGPIASLNITRRPLTTVQLIEKRLFDIVA
                     ATLALIALAPLFTIAALLIKLDSAGPVFFSQRRYGFNQEPFRIYKFRTMTTMEDNSAV
                     KSATRNDPRVTRIGAILRRYSIDELPQLLNVLTGDMSLIGPRPHALAHDQLYERRIAQ
                     YARRHNVKPGITGWAQVRGFRGEIASDEKMRRRVEHDLYYIDNWSFWLDLKIALLTVF
                     SPKTHSDVY"
     gene            1804..>2545
                     /locus_tag="VKU03_00920"
     CDS             1804..>2545
                     /locus_tag="VKU03_00920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WecB/TagA/CpsF family glycosyltransferase"
                     /protein_id="HLJ69846.1"
                     /translation="MRLALKQKGAAANPAAYIGDLPIDAVGLEETARAFIDYCRSAER
                     RRAERPLLSTSVNGQVVSLCAIDRSTAQLFRNADSINADGQPIVTLSRLLCRTPLPER
                     VATSDLFPAVARLAAQSGATFYLLGGAEAVNRAAMERTLALYPRLRIIGRRNGYFSRA
                     EEPAIVEEIASLAPDILWIGLGVPLEQQFCARNLRALRGVGIVKTSGGLFDFLALAKP
                     RAPNWMQRLGFEWLFRMLAEPRRLFLRYL"
BASE COUNT          441 a          819 c          838 g          447 t
ORIGIN      
        1 gtctcggcgc cggaaactca acgaggaaat ctcgaaatcg cgttatttca cgagacttcg
       61 tcgacgacga tggactcgct ctcactttcc ccggatagcc ctgcgccttg cggggagaag
      121 gggagagtca tgctcagtac acgtcgctat gcgtcttcgg cgaaaacacc gtcagcagcg
      181 caatcttcaa gtcgagccag aacgaccaat tgtctatata atagagatcg tgctcgacgc
      241 ggcggcgcat cttttcgtcg ctggcgatct cgccgcggaa gccgcgcacc tgcgcccagc
      301 cggtgatgcc gggtttgaca ttgtgccgcc gcgcatattg ggcgatgcgc cgctcataga
      361 gctgatcgtg ggcgagggcg tgcggccgcg gcccgatcag cgacatgtcg ccggtcagga
      421 cgttgagaag ctgcggcaat tcgtcaatgc tgtagcgccg caaaatcgcg ccgatccgcg
      481 tcacgcgcgg atcgttgcgc gtcgctgatt tcactgcgga attgtcctcc atcgtcgtca
      541 tggtgcggaa tttgtagatg cggaacggct cctgattgaa tccgtaccgc cgctgcgaga
      601 agaagaccgg tccggcgctg tcgagcttga tcagcaaggc cgcgatcgtg aaaagcggcg
      661 ccaaggcgat cagcgcaagt gtcgccgcga cgatgtcgaa gaggcgcttc tcgatgagct
      721 gcaccgtcgt cagtggccgc cgggtgatgt tgaggctggc gatcggcccg agcttggcga
      781 cctccgcgtc tgtgaaacga tcgaggattt cttccggccc gagatggatt tcggcaggcg
      841 tgcggatgaa cgcgtcgacg caggtttcga tcagcctcgc ccgcgcccag gggatggcga
      901 tgaacacgtc gtccgggcgg agcacgcgaa ccgtcgccgc ggcaagcgcg agatcgtcgc
      961 ggacggtgcc gtcgccgtcg cgaatcaccg cggccgagac gacctccatg ccggaacggt
     1021 cgagatcgta ccgctcggcg aagctcgcca gttgcgtttc aagtccgacc aggacgaggc
     1081 gccgccgcgg cagtaagcgg cgctttttgg ccgccgcgat cagcgcgacc aagctggcgc
     1141 gcgcgccgcc gagcgcgaga aatccggcga catagagaac cgcggtcgcg ccgcgcgaga
     1201 agacttccgt cgtcttggtg acgaaaccga gcgccagcac gcaaaaaaag gcgatgttcc
     1261 aggtggaaag gcaacgcccg atatgcccct ccatctgcgc gtattgcttg atgtcgtatt
     1321 cgccgcgctg cgcgttgagc gcgacgacta cggcgccgag gacgaggccg acctcgacga
     1381 cagattcggc gcgcggaaaa aagccgaacg tcgctagccg gtacagaatg tgggacgccg
     1441 ccgcggcggc gacgatcgcg ccaaaatccg ccgccgccgc gacatagctg aagccgacct
     1501 ccaccgcgag catgggcgcg ccggcgcgcc ggcgctggat tgcgccgctt ggcgctgcgc
     1561 gcttttgggc gcggcgcggc catgccggag cgtcgtgaaa ctgaactctc gaatgcttca
     1621 tatcggcaca gatctctacg catgacgcga aacgccgcag gggaggcggc ggtcggcgtt
     1681 tgagcggcaa gcggcgtgcc gcggcctccc gccgccgcct cgttctcgcc gccaagtgtg
     1741 tcgtttcggg aggcacgccg cttgccggtt ggcgacgacg acaaagaaat ccggaacctt
     1801 ccgatgcggt tggcgctgaa acagaaaggc gcggcggcca atccggccgc ctatattggc
     1861 gatctgccga tcgacgcggt tggcctcgaa gagacggcgc gcgccttcat cgattattgc
     1921 cgcagcgccg agcggcgccg ggccgagcgg ccgcttctct cgacctcggt caacggccag
     1981 gtcgtgtcgc tctgcgcgat cgaccgcagt acggcgcaat tattccgcaa cgccgattcg
     2041 atcaacgccg atggccagcc gatcgtgacg ctatcgcgcc ttctttgccg cacgcctttg
     2101 ccggagcggg tcgcgaccag cgatcttttt ccggccgtcg cccggctcgc cgcccaatcg
     2161 ggcgcaacgt tttacctgct cggcggggcc gaagcggtca atcgcgccgc aatggagagg
     2221 acgctcgctc tttatccgcg cctgcgcatt atcggccggc gcaacggcta tttctcgcgc
     2281 gccgaggagc cggcgatcgt cgaggagatc gccagcctgg ccccggacat tctctggatc
     2341 ggactcggcg ttccgctcga gcagcaattc tgcgcccgca accttcgcgc gcttcgcggc
     2401 gtcgggatcg tcaagacgtc gggcggcctg ttcgattttc tcgctctcgc caagccgcgc
     2461 gcgccgaatt ggatgcagcg gctcggcttc gaatggctgt ttcggatgct cgccgagccg
     2521 aggcggctgt ttcttcgcta tctga
//
LOCUS       DATGJW010000031         9383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_523847, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000031 DATGJW010000000
VERSION     DATGJW010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9383
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_523847"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3122)
                     /locus_tag="VKU03_00925"
     CDS             complement(<1..3122)
                     /locus_tag="VKU03_00925"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLJ69847.1"
                     /translation="MRRSVPSLIAEAAASARPAPAKRPRAARRDRPEAAPVLSELFHP
                     AVAAWFARHFAAPTAAQSEAWPAIKSGRHTLIAAPTGSGKTLAAFLAAIDDLVRQGLA
                     GALRDETQIVYVSPLKALSNDIHRNLDIPLAGIRRQLAEMGLPDVEIRAVVRTGDTPA
                     AERERMRRKPPHILVTTPESLYVLLGSESGRAMLATARSVIVDEIHAVASNKRGAHLS
                     LSLERLAALCGDKLLRVGLSATQNPIEAVAHLLVGAGPEGAPAAEAAIIDTGHQRARD
                     LAIEIPEAPLEAVMSADVWRLVYDKLARLIEDHRTTLVFVNTRRMAERVAHELTARLG
                     EKALTAHHGSMAKEHRLAAERRLKNGELKALVATASLELGIDIGEVDLVCQLGSPRSI
                     AAFLQRVGRSGHAVGATPKGRLFPLSRDDLVECAALLDSVRRGELDRLAIPHQPLDAL
                     AQQIAAEVAAKDWPESELYALYRRAWPYRALKREDFGAVVHMLAEGFTTRRGRRGALL
                     HYDAVNHMLRGRRGARTTALTSGGMIPDTADYQVLLEPENQVIGSVNEDFAVESMAGD
                     VFLLGNASYRILRVERGTVRVEDAHGQPPTIPFWLGEAPGRSDELSAAVSRLRETTAQ
                     KLRLDASGAAARTWLVGALGLSEAAATQLVEYLAAAFGALGCLPTQETIVFERFFDEA
                     GGMQFIVHSPFGSRVNRAWGLALRKRFCRKFNFELQAAATEDNIVLSLTTAHSFELAE
                     VARYLHSNNVRHVLIQAMLDSPMFTTRWRWVASASLALPRYRAGKKVPPQFMRMQAED
                     LIAAVFPDQIACAENLVGEREVPDHPLTDQAIDDCLHEAMDIDGLERLLRRIEAGDVR
                     IVACDLTQASPLALEVLSARPYAFLDDAPLEERRTQAVMARRWLDPESAADLGRLDGE
                     AIERVRAEAWPEPGDAEELHDALLWLGCLTEAEAKAAPDWLFWLEALAKARRATRLVT
                     PRAMLWVAAERLNQVQAIWPDAARQPDIAPPADQAQAVWSAEQALVEIVRGRLEGLGP
                     VTETALAAPLG"
     gene            3342..4652
                     /locus_tag="VKU03_00930"
     CDS             3342..4652
                     /locus_tag="VKU03_00930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF763 domain-containing protein"
                     /protein_id="HLJ69848.1"
                     /translation="MARRAGSADLPLHGGRVPAWLGERMTRLGAVMCEAIVHHYGRDE
                     LLRRLAHPFWFQSFGCVMGMDWHSSGITTSVLGALKRGLQPLSGELGLHVCGGRGKHS
                     RKTPQELVAIGERKGFDGAKLATASRLVAKVDSAAVQDGFALYLHGFIVADDGHWVVV
                     QQGMNGDRGQARRYHWLSEGLKSFVEEPHAAIDGRSQGEIVNLTDRRAALSRRGQVEM
                     LQSLGPDRIVAELASLEGEARAEPAADPQLTLPHLIMPAHHGVRPKDVLARRLHGTLA
                     AAAERGPQDFSELLLAPGVGARTVRALAMVAEVVHGAPYRFSDPARFALAHGGKDRHP
                     FPVPLSVYDQTIAVLKSAVQKAKLGREEELGALKRLDDQARLLERVATGPTVDEHIAQ
                     ERRDSHLYGGRSVFGWEKAPEELSSPAKRGRGTARRAVEGTAGG"
     gene            complement(4656..6035)
                     /gene="glcF"
                     /locus_tag="VKU03_00935"
     CDS             complement(4656..6035)
                     /gene="glcF"
                     /locus_tag="VKU03_00935"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcF"
                     /protein_id="HLJ69849.1"
                     /translation="MQTNFPPELLADPHMRASEKILRSCVHCGFCTATCPTYLLLGDE
                     LDSPRGRIYLIKDMLENQKPASAEVVTHIDRCLSCLSCMTTCPSGVHYGHLVDHARAY
                     IEETYRRPLLDRALRGALALILPSPRLFRLALLAAKAGRPLAPVLARLPKLGQKLAVM
                     LALAPRQLPKRRAVGPQAFAAQNGRAKQRVALLTGCAQSVLTPETNAAAIELLTRFGI
                     EVVLAEGEGCCGSLVHHMGHEARALQQARANIDAWMREIEGRGLDAVLITASGCGTTV
                     KDYGFMLRDDPAYAEKAARVSALAKDVTEYLATLDLAFVENEKLTVAYHSACSMQHGQ
                     KVTEQPKALLRKAGFSVREPAESHICCGSAGTYNILQPEIAARLRERKLKNIHRLKPD
                     VIASGNVGCITQLALGANVPIVHTVELINWATGGAKPEAMRLANPPPSETGEGDHAKH
                     GGGGRGPSG"
     gene            complement(6042..7232)
                     /gene="glcE"
                     /locus_tag="VKU03_00940"
     CDS             complement(6042..7232)
                     /gene="glcE"
                     /locus_tag="VKU03_00940"
                     /EC_number="1.1.99.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycolate oxidase subunit GlcE"
                     /protein_id="HLJ69850.1"
                     /translation="MTQAAAFAPESEEEAAAIVRDAAARGQKYDITGGRARAGLGRPR
                     RRGANLSTARLSGIVFYEPAEMVIALRAGTPVAEVEKLLAARGQMLPFEPMDHRPLYA
                     AGGEPTIGGIVAGNISGPRRISAGAARDSLIGVRIVNGRGEAIKSGGRVMKNVTGLDL
                     VKLVCGAHGTLGLVTEATFKLLPKPEREATVVIRRLDDKSAIAAMTAALGSPYGVSGA
                     AHLTPGMGREFARTFFRVEGFASSVEHRVGELIKLLAPFGAKHALVEEESRRLWRGVR
                     DAEFLAEPRERAVWRVSLAPSKGADFVAKLGPAALAHFYDWGGGLVWLASEASRAAGE
                     TIRAALAPFGGHATLTRAPDELRESVDVFEPLSPALTKLTAGIKASFDPDRIFNFERM
                     YRGI"
     gene            complement(7229..8665)
                     /locus_tag="VKU03_00945"
     CDS             complement(7229..8665)
                     /locus_tag="VKU03_00945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017959129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLJ69851.1"
                     /translation="MSHIAFPPPDPSILARRDAILAGLAAALPNGGLIVAEEERRAFE
                     TDALTAYRKLPLAVALPSSIDEIAAILKFCRRAGVNAVPRGAGTSLSGGAIPQEDAIV
                     IGLSKMNRILEINLADRYARVEAGVTNLAISEAVAADGFFYAPDPSSQLACTIGGNIG
                     MNSGGAHCLKYGVTTNNLLGLKVVLLDGEIVELGGAQLDSGGLDLMGLMTGSEGQLGV
                     VAEAIVRLLPAPEGARPVLFGFDSAQAAGACVAAIIGAGILPVAMEYMDKLAIEICEA
                     FAHAGYPLDVEAMLIIEVEGSAKEIDAELAKILAIAKEHGVKAVRECMSAMEAAQIWK
                     GRKSAFGATGRVADYICMDGTVPTSRLPEALKRTGEIVAAHGLRVANVFHAGDGNMHP
                     LILYNVNDPKEQAKAEAAGEDILKMCVDLGGCLTGEHGVGIEKRDLMGHQFSPADLAQ
                     QMRVRAVFDPDWRLNPAKVFPLAGRIAA"
     gene            complement(8736..9155)
                     /locus_tag="VKU03_00950"
     CDS             complement(8736..9155)
                     /locus_tag="VKU03_00950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ69852.1"
                     /translation="MFVDASAILAILLDEADGPKLASALQSAKRRKPVTSVLAAWEAA
                     VGLNRKKGLPIEEAEARILEFIETAGVDLVGVSAAELSAALRAFDRYGRHRYPEADRN
                     KALNLADCFHYACAKARRMPILHQDAGLALTDAPSVF"
     gene            complement(9155..9382)
                     /locus_tag="VKU03_00955"
     CDS             complement(9155..9382)
                     /locus_tag="VKU03_00955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HLJ69853.1"
                     /translation="MRSDEVDRLAGKLARRKGVTKTEAVRLALLNELRRVEDAVPLWE
                     RLAPLRRKIAAHPDTGLEADKAFFDELSGAF"
BASE COUNT         1551 a         3149 c         3178 g         1505 t
ORIGIN      
        1 ccgagcggcg cggcgagcgc ggtctcggtg acgggcccta agccctccag ccggccgcgc
       61 acgatctcga ccagcgcctg ctcggccgac cacaccgcct gagcctggtc cgccggcggc
      121 gcgatgtccg gctggcgcgc cgcatccggc cagatcgcct gaacctggtt gagccgctcg
      181 gcggccaccc acagcatcgc ccgcggcgtg acgagacggg tggcgcgcct ggccttcgcc
      241 agcgcttcca gccagaaaag ccagtccggc gccgccttcg cttccgcctc ggtgagacag
      301 ccgagccata acagcgcgtc gtgaagctct tcggcgtcgc ccggctcggg ccaggcttcg
      361 gcgcggacac gctcgatcgc ctccccgtcg agccggccga gatcggcggc gctctccgga
      421 tcgagccagc gccgcgccat caccgcttgc gtgcgccgct cctcgagcgg cgcgtcgtcg
      481 aggaaagcgt agggccgggc ggacaggacc tccagcgcca gcggcgacgc ctgcgtcagg
      541 tcgcacgcga cgatgcggac gtcgccggcc tcgatccgcc gcagcagccg ctccagcccg
      601 tcgatatcca tcgcctcgtg caagcaatcg tcgatcgcct gatcggtgag cgggtgatcg
      661 ggcacttcgc gctcgccgac gagattttct gcgcaagcga tctgatcggg gaacaccgcg
      721 gcgatcaggt cctcggcctg catgcgcatg aattgcggcg gcactttctt ccccgcccga
      781 tagcgcggca aagcgagcga ggcgctcgcc acccatcgcc agcgggtggt gaacatcggc
      841 gaatcgagca tcgcctggat cagcacatgg cggacgttgt tcgaatggag atagcgcgcg
      901 acctcggcga gctcgaaact gtgggcggtg gtcagcgaaa gtacgatatt gtcctccgtc
      961 gccgccgcct gcagctcgaa gttgaacttg cggcagaaac gcttgcgcag cgccaagccc
     1021 caggcgcggt tgacgcggct gccgaacggc gaatggacga tgaattgcat gccgcccgct
     1081 tcgtcgaaga agcgctcgaa gacgatcgtc tcttgcgtcg gcaggcagcc gagcgcgccg
     1141 aaagcggcgg cgagatattc gacgagctgc gtcgccgcgg cctcgctcaa gccgagcgcg
     1201 ccgacgagcc atgttcgggc cgccgcgccc gatgcgtcga gcctgagctt ctgcgccgtc
     1261 gtttcgcgca gccgcgacac cgcggcggaa agctcgtcgc tgcggcccgg cgcttcgccg
     1321 agccagaagg gaatcgtcgg cggctggcca tgggcgtcct cgacccgcac cgtcccgcgc
     1381 tcgacgcgca ggatgcgata ggaggcgttg ccgagcagga agacgtcgcc ggccatgctt
     1441 tcgaccgcga aatcctcgtt gacgctgccg atgacctggt tttccggctc gagcagcact
     1501 tggtaatcgg cggtgtcggg gatcatgccc cccgatgtga gcgccgtcgt tcgcgcgccg
     1561 cgccttccgc gcagcatgtg attgactgcg tcatagtgaa ggagcgcgcc gcgccttccg
     1621 cgccgcgtcg taaagccctc ggcgagcata tgaacgaccg cgccgaagtc ttcgcgcttc
     1681 aacgcgcgat agggccaggc gcggcgatag agcgcgtaga gctcgctttc gggccaatcc
     1741 ttcgccgcga cttccgcggc gatctgctgc gccaaggcgt cgagcggctg atgcggaata
     1801 gccagccggt cgagctcgcc gcggcggacg ctgtcgagca aggcggcgca ttcgacaaga
     1861 tcgtcgcgcg acagcgggaa gagccgcccc ttcggcgtcg cgccgacggc gtggccggag
     1921 cggccgaccc gctgcaggaa ggcggcgatc gagcgcggcg agccgagctg gcagacgaga
     1981 tcgacctcgc cgatatcgat gccgagttcg agcgaggcgg tggcgaccag cgctttcaat
     2041 tcgccattct tcaagcgcct ctcggcggcg aggcgatgct ccttggccat gctgccgtga
     2101 tgagcggtca gcgccttctc gccgagccgc gccgtcaatt catgggcgac gcgctcggcc
     2161 atccgccgcg tgttgacgaa gaccaacgtc gtgcgatggt cttcgatgag ccgtgcgagc
     2221 ttgtcgtaga ccaggcgcca gacatcggcc gacatcaccg cttcgagcgg cgcctcgggt
     2281 atttcgattg caaggtcgcg cgcgcgctga tggccggtat cgatgatcgc cgcttccgcc
     2341 gccggcgcgc cctccggccc ggcgccgacg agaagatgcg ccacggcctc gatcggattc
     2401 tgcgtcgcgg aaagaccgac gcgcaaaagc ttgtcgccgc acaaagcggc gaggcgttcg
     2461 agcgacaggc tcaaatgcgc gccgcgcttg ttcgacgcga cggcgtgtat ctcgtcgacg
     2521 atgacgctgc gcgcggtcgc cagcatggcg cggcccgatt cggagccgag cagcacatag
     2581 agcgattccg gcgtcgtcac gagaatatgc ggcggcttgc ggcgcatgcg ctcgcgctcc
     2641 gctgcgggcg tatcgccggt gcgcacgacg gcgcgaatct cgacatcggg caggcccatc
     2701 tcagccaatt gccggcggat gccggcgagc ggaatgtcga gattgcgatg gatgtcgttc
     2761 gacagcgcct tcaagggcga cacatagacg atctgggtct cgtccctgag cgcgccggcg
     2821 aggccttggc ggacgagatc gtcaatcgcg gcaagaaagg cggcgagcgt cttgccggag
     2881 ccggtcggcg cggcgatcag cgtgtggcgg ccggatttga tcgccggcca ggcctcgctt
     2941 tgcgccgcgg tcggcgcagc gaaatggcgg gcgaaccagg cggcgacggc ggggtggaaa
     3001 agctccgaca aaacgggcgc cgcctcgggc cggtcgcgcc gggcggcgcg agggcgcttc
     3061 gccggcgccg ggcgggcgct ggcggccgct tcggcgatga gggagggaac ggatcgccgc
     3121 atcaaaactc ctcgccgcgc ggccgctcca agccgagcgg gggcgcttct gcagcaacac
     3181 aaccgggtgg atcgatctta tttaagaccg gcccgcctga gaacaagcgt ccttccaagc
     3241 gcgtttcgag gctctgccgc ctcttatcaa agtcaaaaca gcctagccta aacgatggat
     3301 tttctctaga acattccagg aacagtttcg tgaggtccac gatggcacgc cgcgccggca
     3361 gcgccgatct tcccttgcat ggcggccgtg ttcccgcctg gctcggcgag cggatgacgc
     3421 ggctcggcgc ggtaatgtgc gaggcgatcg tccatcatta cggccgcgac gaattgctgc
     3481 gccggctcgc ccatcctttc tggttccagt ccttcggctg cgtcatgggc atggactggc
     3541 attcgtccgg gatcacgacc agcgtgctcg gcgcgctgaa gcgcggcctg cagccgcttt
     3601 ccggcgaatt aggcctgcat gtctgcggcg gccgcggcaa gcattcgcgc aagacgccgc
     3661 aagagctcgt cgcaatcggc gaaaggaaag gcttcgacgg cgcgaagctc gccacagcga
     3721 gccgcctcgt cgccaaggtc gacagcgccg ctgtgcagga cgggttcgcc ctctacctgc
     3781 acggcttcat cgtcgccgac gacggccatt gggtcgtcgt gcagcagggc atgaacggcg
     3841 atcgcggcca ggcgcggcgc tatcactggc tgtcggaagg cttgaagagc ttcgtcgagg
     3901 agccgcacgc cgcgatcgac gggcgctcgc aaggcgagat cgtcaacctc accgaccgcc
     3961 gcgccgcctt atcgcggcgc gggcaggtcg agatgctgca aagcctcggg ccggaccgga
     4021 tcgtcgcgga actcgcttcc ctcgaaggcg aggcgcgcgc cgagccggcc gcggacccgc
     4081 agctaactct gccgcacctg atcatgccgg cccatcacgg cgttcggccg aaagacgttc
     4141 tggcgcggcg cctgcacgga acgctcgccg cggcggctga gcgtgggccg caggattttt
     4201 ccgagcttct gctcgcgccg ggcgtcggcg cgcgcaccgt gcgcgcactg gccatggtgg
     4261 cggaagtcgt gcatggcgcg ccctatcgct ttagcgaccc ggcgcgtttc gcgctcgccc
     4321 atggcggcaa ggaccggcat cccttccccg tgccgctcag cgtctacgat cagacgatcg
     4381 ccgtgctgaa atccgccgtg caaaaggcca agctcggccg cgaggaggag cttggcgcgc
     4441 tgaagcggct cgacgatcag gcgcggcttt tggagcgcgt cgcgacagga ccgacggtcg
     4501 atgagcacat cgcgcaagag cggcgcgatt ctcacctcta tggcggccgc agcgtgttcg
     4561 gatgggagaa agcgccggaa gaactgtcct cccccgcgaa gcgagggagg gggactgctc
     4621 gaagggcggt ggaggggacc gccggcggtt gaggattatc ctgatggacc gcggccccct
     4681 ccaccatgct tcgcatggtc cccctccccc gtctccgacg ggggaggatt ggccagacgc
     4741 atcgcctccg gcttcgcgcc gccggtcgcc cagttgatca actcgactgt atggacgatc
     4801 gggacattcg cgccgagcgc gagctgcgtg atgcaaccga cattgccgct ggcgatcacg
     4861 tccggcttga ggcgatggat gttcttgagc ttgcgctcgc gtaagcgcgc cgcgatctcg
     4921 ggctgcagga tgttgtacgt cccggccgag ccgcagcaaa tatggctctc ggccggctcg
     4981 cgcaccgaaa agcccgcctt gcgcagcagc gctttcggtt gctcggtcac tttctggccg
     5041 tgctgcatcg aacaggccga gtgataggcg acggtcagct tctcgttctc gacgaaggcg
     5101 agatcgagcg tcgccagata ttcggtcacg tccttggcca gcgccgaaac ccgcgccgcc
     5161 ttctcggcat aggcgggatc gtcgcgcagc atgaagccgt aatccttgac tgtcgtgccg
     5221 cagcctgatg ccgtgatcag gacggcgtcg aggccgcgcc cttcgatctc gcgcatccag
     5281 gcgtcgatat tggcgcgcgc ttgctgaagc gcccgcgcct catggcccat atgatggacg
     5341 agcgagccgc aacagccctc gccttcagcg aggacgacct cgatgccgaa gcgcgtcaaa
     5401 agctcgatcg ccgccgcatt ggtctcaggc gtcaagaccg attgcgcgca gcctgtcagc
     5461 aaggcgacgc gctgtttcgc acgtccgttc tgcgcagcga aggcttgcgg gcccacggcg
     5521 cgccgcttcg gcaattgccg cggcgccagc gcgagcatga ccgcgagctt ctggccgagc
     5581 ttcggcaggc gcgccaaaac cggcgcaagg ggccgtccgg ctttggcggc gagcaaggcg
     5641 agccggaaca ggcgcggcga gggcaggatc agagccaaag cgccgcgcag cgcgcggtcg
     5701 aggagcggcc gtcggtacgt ttcttcgata taggcgcggg cgtgatcgac aaggtggccg
     5761 taatgcacgc ccgagggaca ggtggtcatg caagagagac aggataggca gcggtcgata
     5821 tgcgtaacca cctcggccga ggccggcttc tggttctcca gcatgtcctt gatgagatag
     5881 atgcggccgc gcgggctgtc gagctcgtcg ccaagcagca ggtaggtcgg gcaggtcgcg
     5941 gtgcagaagc cgcaatggac gcaggagcgg aggattttct ccgacgcccg catatgcgga
     6001 tcggcgagaa gctcaggcgg gaaattggtc tgcatgccgc ctcaaatccc gcgatacatc
     6061 cgctcgaaat tgaaaatccg gtccggatcg aagctcgcct tgatcccagc cgtaagcttc
     6121 gtcagcgccg gcgacagcgg ctcgaaaaca tcgacgctct cgcgcaattc gtccggcgcg
     6181 cgagtgagcg tcgcatgccc gccgaacggc gcgagcgcgg cgcggatcgt ctcgccggcg
     6241 gcgcgggaag cctcgctcgc cagccagacc aggccgccgc cccaatcgta aaaatgcgcc
     6301 agcgcggccg ggccgagctt ggcgacgaaa tcggcgccct tcgacggcgc gagcgagacg
     6361 cgccagaccg cccgctcgcg cggctcggcg agaaattcgg cgtcgcggac gccgcgccat
     6421 agacggcgcg attcctcctc gaccaaggcg tgcttggcgc cgaacggcgc cagcagtttg
     6481 atcagctctc cgacccggtg ctcgaccgag ctggcgaagc cttcgacgcg gaagaacgtg
     6541 cgagcgaatt cgcggcccat gcccggcgtg agatgagccg cgccgctgac gccatagggc
     6601 gagccgagcg cggccgtcat cgccgcgatc gcgcttttgt cgtcgaggcg gcgaatgacg
     6661 acagtcgctt cgcgctccgg cttcggcaga agcttgaacg tcgcctcggt cacgaggccg
     6721 agcgttccat gcgcgccgca gaccagcttg acgagatcga ggccggtgac gtttttcatc
     6781 acccggccgc cgctcttgat cgcttcgccg cggccattga cgatcctgac gccgatcagg
     6841 ctgtcgcgcg ccgcgccggc gctgatccgg cgcggccccg atatgttgcc ggcgacaatg
     6901 ccgccgatcg tcggttcgcc gcccgccgca tagagcggcc gatggtccat cggctcgaaa
     6961 ggcagcatct ggccgcgcgc ggcgagcaac ttttcaacct cggccaccgg cgttccggcg
     7021 cggagcgcga tcaccatctc ggccggctcg tagaacacga ttccgctgag ccgggccgtc
     7081 gagagattcg cgccgcgccg cctgggccgg ccgagcccgg cccgcgcgcg gccgccggtt
     7141 atgtcgtact tctgccctcg cgcggccgcg tcgcgaacga tcgccgccgc ctcctcttcg
     7201 ctctccggcg caaaagccgc tgcctgcgtc acgcggcgat ccttcccgcc aagggaaaaa
     7261 ccttggccgg attgagccgc caatcgggat cgaacacggc gcgcacgcgc atctgctggg
     7321 caagatcggc ggggctgaat tgatggccca tcagatcgcg cttctcgatg ccgacgccgt
     7381 gctctccagt caggcagccg ccgagatcga cgcacatttt cagaatatcc tcgcccgccg
     7441 cttcggcttt ggcctgctct ttcgggtcgt taacgttata aagaatcagc ggatgcatgt
     7501 tgccgtcgcc ggcgtgaaag acattggcga cgcgcaagcc gtgcgcggcg acgatctcgc
     7561 ccgtccgctt cagcgcttcc ggcaggcggc tggtcggcac ggtgccgtcc atgcagatat
     7621 agtcggcgac gcggccggtg gcgccgaagg ccgatttgcg gcctttccag atttgcgcgg
     7681 cctccatcgc cgacatgcat tcgcgcaccg ccttgacgcc gtgctctttg gcgatggcga
     7741 ggattttcgc cagctcggcg tcgatctcct tggccgagcc ctcgacctcg atgatcagca
     7801 tcgcctcgac gtccagcgga tagccggcat gggcgaaagc ctcgcagatt tcgatcgcca
     7861 gcttgtccat atactccatc gccaccggca ggatgccggc gccgataatc gcggcgacgc
     7921 aggcgccggc ggcctgcgcg gaatcgaagc cgaacagaac cggccgcgcg ccttcggggg
     7981 ccggaagcaa gcgcactatg gcttccgcaa cgacgccgag ctggccttcc gagccggtca
     8041 tcaggcccat cagatcgagg ccgccggaat cgagctgcgc gccgccgagc tcgacgatct
     8101 cgccgtcgag cagcacgact ttcaggccga gcaggttgtt ggtcgtgacg ccgtatttga
     8161 ggcaatgggc gccgccggaa ttcatgccga tattgccgcc gatcgtgcag gcgagctgcg
     8221 aggacgggtc gggcgcgtag aaaaatccgt ccgccgcgac ggcctcggaa atcgccagat
     8281 tggtgacgcc ggcctcaacc cgggcgtagc ggtcggcgag gttgatttcg aggatgcggt
     8341 tcatcttcga aaggccgatg acgatcgcat cctcctgcgg gatggcgccg ccggacaaag
     8401 aggtgccggc gccccgcggc accgcgttga cgccggcgcg ccggcagaat ttgaggatgg
     8461 cggcgatctc gtcgattgag ctcggcagcg ccacggccag cggcagcttg cgataggcgg
     8521 tgagggcgtc ggtttcaaag gcgcgacgct cctcctcggc gacgatcagg ccgccattcg
     8581 gcagcgccgc cgcgaggccg gccaggatcg cgtcccgccg cgcgaggatg gacgggtcgg
     8641 gaggggggaa ggcgatgtgc gacatgggga gctcaatggt cgcgctgaat gccggatttt
     8701 actctcgctc gcgcgccggg gcgagcgaca aggcttcaaa acacgcttgg cgcatccgtc
     8761 aacgccaatc cggcgtcttg gtgcaaaatc ggcatgcggc gagccttggc gcaagcgtaa
     8821 tgaaaacagt cagcaagatt tagcgccttg tttcgatccg cctctggata gcgatggcgg
     8881 ccatagcggt cgaaagctcg caacgccgcg gagagctccg ccgccgagac gccgacaagg
     8941 tcgacgccgg ctgtttcaat gaactccaag attctggcct ccgcttcctc gattggcagg
     9001 ccctttttcc gattgagccc aaccgccgcc tcccaagccg ccagaacgga ggttacgggt
     9061 tttcgtcgtt tcgcggattg cagcgcgctc gcgagcttcg ggccgtccgc ctcgtcgaga
     9121 aggatcgcaa ggatcgcgga ggcgtcgacg aacatcagaa cgcgccgctg agctcgtcaa
     9181 aaaacgcctt atccgcttcg agacctgtat cgggatgagc ggcgatctta cggcggaggg
     9241 gggccaagcg ctcccacaag ggaaccgcgt cctccactcg gcgtaactcg ttcagtaacg
     9301 ccagccgcac cgcttcggtt ttggtcacgc ctttccgcct ggccagcttg ccagcaagcc
     9361 gatcgacttc gtcgcttcga atg
//
LOCUS       DATGJW010000032         6580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1249688, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000032 DATGJW010000000
VERSION     DATGJW010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6580
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1249688"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VKU03_00960"
     CDS             <1..664
                     /locus_tag="VKU03_00960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HLJ69854.1"
                     /translation="KVAIIAGNVATRDGAQALIDAGADAIKVGIGPGSICTTRIVAGV
                     GVPQLSAIMEAAEAARRADIPVIGDGGIKYSGDLAKAIAGGADCAMIGSLLAGTDESP
                     GEVFLYQGRSYKSYRGMGSLGAMARGSADRYFQQDIKDQMKLVPEGVEGQVPHRGPAG
                     AILHQLVGGLRAAMGYVGARDIAEFQAKAQFMRISSAGLRESHVHDVAITRESPNYPS
                     RL"
     gene            774..1007
                     /locus_tag="VKU03_00965"
     CDS             774..1007
                     /locus_tag="VKU03_00965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69855.1"
                     /translation="MTKIVLGALLAVAVATSANAGFSVSPITSDPGVTRVAEGCGPGM
                     WRGPHGRCHPMFNGRACPRGYHLGPHHRRCWPD"
     gene            1178..1435
                     /locus_tag="VKU03_00970"
     CDS             1178..1435
                     /locus_tag="VKU03_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69856.1"
                     /translation="MTIRFIVGAVFAVSLVSSANAAMLAPPVTNGDSGVEKVYGGCGP
                     YGHRGPYGGCRPGGQWGGYRWGRPCPPGFHLGRYGHRCWPN"
     gene            complement(1550..1705)
                     /locus_tag="VKU03_00975"
     CDS             complement(1550..1705)
                     /locus_tag="VKU03_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69857.1"
                     /translation="MIRTVRCLAFAALALSLAGCAPPPPPPSAQIVAPAKKPLDAKTQ
                     LETGRTY"
     gene            1799..3091
                     /locus_tag="VKU03_00980"
     CDS             1799..3091
                     /locus_tag="VKU03_00980"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RsmB/NOP family class I SAM-dependent RNA
                     methyltransferase"
                     /protein_id="HLJ69858.1"
                     /translation="MTPGARISAAIDILVAIEEQRRPAAEAIKEWGFAHRFAGSRDRS
                     AIAGLVYDALRRKASSTWLMGAPSPRAAMIGALKQARRMSADAIADLFTGEAHAPARL
                     SAAERERLTAATLDAAPDYVRGDFPEWLAPAFAESFGAKAAAEGEALAQRAPVDLRVN
                     TLKGDRAKALAALAHLSPVEAPFSPIGLRIEIGADGRGPALAAEPAYVKGLVEIQDEG
                     SQLAALLSAASPGQQVLDLCAGGGGKSLALAAMMKNQGQIYATDSDGRRLMPIYDRLE
                     RAGARNVQVRAPKGARDILGDLVGRCDMVLIDAPCSGSGAWRRNPDAKWRMRPGALAQ
                     RIKEQQQTLEQASRYVKKGGRLVYATCSLFKAENEDRIAEFLSRRDDFLPVEAGHLAR
                     AAGLPALAAHGSKLGAGLRLSPLETGTDGFYIAALARS"
     gene            3165..4172
                     /gene="lipA"
                     /locus_tag="VKU03_00985"
     CDS             3165..4172
                     /gene="lipA"
                     /locus_tag="VKU03_00985"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HLJ69859.1"
                     /translation="MTVVLDLLNDDPRRRSAERHPEKAHRPDQPIERKPPWIRVKAPG
                     SRQFAETSRIVRTNKLVTVCEEAACPNIGECWDKKHATFMIMGDVCTRACAFCNVRTG
                     LPGALEAGEPERVAEAVAKLGLAHVVVTSVDRDDLADGGAEHFARTIRAIRAAAPATT
                     IEVLTPDFLRKDGALEIVVGARPDVFNHNLETVPSKYLTVRPGARYFHSVRLLQRVKE
                     IDPSMFTKSGIMVGLGEERHEVLQLMDDLRSAEVDFLTIGQYLQPSRKHHRVVRFVTP
                     DEFASYAAIGAAKGFLLVSSTPLTRSSHHAGDDFGRLKAARVARFAPAAASIDPLGAK
                     L"
     gene            4197..4272
                     /locus_tag="VKU03_00990"
     tRNA            4197..4272
                     /locus_tag="VKU03_00990"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:4230..4232,aa:Lys,seq:ttt)
     gene            4734..5009
                     /locus_tag="VKU03_00995"
     CDS             4734..5009
                     /locus_tag="VKU03_00995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69860.1"
                     /translation="MPSRFTSGAYGPELVKVMTGAFDRAWDDFEPRPRNLNLARSLMA
                     SAIIEAVEAGDRDEQSLVRKATVTLIKAIKVDPDLLNAAAPPDLSNV"
     gene            5143..5409
                     /locus_tag="VKU03_01000"
     CDS             5143..5409
                     /locus_tag="VKU03_01000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69861.1"
                     /translation="MARISKICGSITLFFVIASGGAALADVPRLHCGVLSHPVANLGA
                     VNPRHAYWCKSNVAGYLGEDRSVPIRPYLAHRYRRRHRKRVWID"
     gene            complement(5410..6102)
                     /locus_tag="VKU03_01005"
     CDS             complement(5410..6102)
                     /locus_tag="VKU03_01005"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HLJ69862.1"
                     /translation="MFENGKRRSLAAAAGVGAAGFVILVATTVVARGPDIGNPYELLL
                     PNYSKVAPQTAPAPSIPMTSPRRRSIAAIAGHPVCVRLCDGAYFPISTGNGEEAQCRN
                     LCPDAATAIYQAPNGSDDIGEAVSKTGKRYSELPTALRYRSGLDAACVCHRSIAPHYS
                     LAEDSTLRKGDYVMTRNGLAVFEGGAALPHHAADFVALDKARLSAEYTRSAEALHGPK
                     GAPRRAIRITTP"
     gene            6366..>6580
                     /locus_tag="VKU03_01010"
     CDS             6366..>6580
                     /locus_tag="VKU03_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HLJ69863.1"
                     /translation="MKVTSPDIKPGAKIAEEQVANGFGCEGKNISPALSWSGAPAGAK
                     SFAVTMYDPDAPTGSGFWHWVVFNIPAG"
BASE COUNT         1121 a         2117 c         2168 g         1174 t
ORIGIN      
        1 caaggtggcg atcatcgccg gcaatgtcgc cacgagagac ggcgcgcagg ctctgatcga
       61 cgccggcgcg gacgcgatca aggtcggcat cggcccgggc tcgatctgca cgacgcgcat
      121 cgtcgccggc gtcggcgtgc cgcagctcag cgcgatcatg gaggcggcgg aagcggcgcg
      181 ccgcgccgac atcccggtga tcggcgacgg cggaatcaaa tattccggcg acctcgcaaa
      241 agcgatcgcc ggcggcgccg attgcgcgat gatcggttcg ctgctcgccg gcaccgacga
      301 aagcccaggc gaggttttcc tctatcaagg ccgttcgtac aaatcctatc gcggcatggg
      361 ctcgctcggc gccatggcgc gcggctcggc cgaccgctat ttccagcagg acatcaagga
      421 ccagatgaag ctcgtgccgg aaggcgttga aggccaagtg ccgcatcgcg ggccggccgg
      481 cgcaatcctt caccaactgg tcggcggctt gcgggcggcg atgggctatg tcggcgcgcg
      541 cgacatcgcc gagttccagg ccaaggcgca attcatgcgc atctcgtcgg cgggcttgcg
      601 cgagagccat gtccacgatg tcgcgatcac ccgcgagagt ccgaactatc cgagccggct
      661 gtaacgattt tccgcacgcc gattggcgtt ccctccgccg gatgctacgg cttcgccgtc
      721 ccgattcgct gaggcgcgat attgggcgaa tcgaacgagg acggaggaag cagatgacga
      781 aaattgttct cggcgcgctg ttggcggtcg cggtcgcgac gtcggcgaat gccggattct
      841 cggtttcgcc catcacaagc gatccgggcg tgacgcgcgt cgccgaaggc tgcggcccgg
      901 gcatgtggcg cggtccgcat ggccgttgcc atccgatgtt caatggccgg gcgtgcccgc
      961 gcggctacca tctgggcccg catcatcgcc gctgctggcc ggactgagtc gtcggcggac
     1021 tttcgctcaa ggagcgaggc gttttcgcct cgcttttttt ataccgtgct cggccgcgaa
     1081 ccccgcagag ttgcactggc cggttttgtt gatactgtct cccgcgccaa acctcaaggg
     1141 cgcgcgcctt tgcgttcccg gagaagggga aatgatcatg accatcaggt ttatcgtggg
     1201 ggcggtattc gcggtcagtc tggtttcatc cgccaacgcg gccatgttgg cgccgccggt
     1261 gaccaatggc gatagcggcg tcgagaaagt gtatggcggc tgcgggccct atggccatcg
     1321 cgggccctat ggcggctgcc gtcccggcgg ccaatggggc ggctatcgtt ggggccgtcc
     1381 atgccctccc ggcttccatc tggggcgcta cggccaccgc tgctggccga actgagcgga
     1441 gtttttctcc agccgggaag agcgaggcca accgcctcgc tctttttttg tttctattac
     1501 cgcgccgagc gccgatttgc gcttcgtccg ccggtcggtc taaacgacgt cagtaagttc
     1561 tgccggtctc aagctgggtt ttggcgtcga gcggcttctt ggccggcgcg acgatttggg
     1621 cgctgggcgg cggaggcggc ggagcgcagc cggcaagcga caaagcgagc gcggcaaacg
     1681 cgagacaacg aacggtccga atcatgacgt ctccaatgga tggggctgcg aagcgccgga
     1741 aggcgcgcgc tcgcgcattc ggcttagacc aagcatcggc ggcgacggtc aaggctttat
     1801 gacgcccggc gcacgtattt ctgcggcgat cgacattctc gtcgcgatcg aagaacagcg
     1861 ccggccggcc gccgaggcga tcaaggagtg ggggttcgcc catcgcttcg ccgggtcgag
     1921 ggaccgatcg gcgatcgccg gcctcgtcta tgacgccttg cggcgaaaag cgtcctccac
     1981 ttggcttatg ggggcgccca gcccgcgcgc ggcgatgatc ggcgcgctca agcaggcgcg
     2041 aaggatgagc gccgacgcca tcgccgatct gttcaccggc gaggctcatg cgccggcgcg
     2101 gctttccgcc gccgagcgcg agcgtttgac ggccgcgact ctcgacgccg cgcccgatta
     2161 cgtccgcggc gattttcccg aatggctcgc gccggctttc gccgagagct tcggcgcgaa
     2221 agccgcggcg gaaggcgagg cgctcgccca gcgcgcgccg gtcgatttgc gggtcaacac
     2281 gctcaaaggc gaccgcgcca aggcgctcgc cgcgctggcc catttgtcgc cggtcgaagc
     2341 gccgttttcg ccaatcggcc tgcgcatcga gatcggcgcc gacggccgtg gaccggcgct
     2401 ggccgccgag ccggcttacg tcaaaggcct cgtcgaaatt caggacgagg gctcgcagct
     2461 tgccgccttg ctgtcagccg cgtcgcccgg ccagcaagtc ctcgaccttt gcgccggcgg
     2521 cggcggcaaa agcctggcgc tggccgcgat gatgaagaat caaggccaga tctatgcgac
     2581 cgattcggac ggaaggcggc tcatgccgat ctacgatcgg ctcgaacggg caggggcgcg
     2641 caatgtccag gttcgcgctc caaagggcgc gagggacatt ctcggcgatc tcgtcggccg
     2701 ctgcgacatg gttctcatcg acgcgccttg cagcggctcc ggcgcctggc ggcgcaatcc
     2761 cgacgcgaaa tggcggatgc ggcctggcgc gctcgcgcag cggattaaag agcaacagca
     2821 aacgcttgag caagcgtcac gatatgtaaa gaagggcggg cgactggttt acgcgacctg
     2881 ctcgctgttt aaggcggaga acgaagaccg catcgccgag ttcttgagcc gccgcgacga
     2941 ttttctcccg gtcgaggccg gccatctcgc ccgcgccgcc ggcttgccgg cgcttgccgc
     3001 tcatggctcg aagcttgggg ctggtcttcg cctcagccca ctcgaaaccg gcacggacgg
     3061 attttatatt gcggctctgg cccgctcgtg accggagcgc tatcgaagag gcgcaaaagc
     3121 gtgagtgagc ctctttcgag gcgcggcgac gccggagctg aaaaatgacg gtcgttctcg
     3181 accttttaaa cgatgatccg cgccgccgct cggccgagcg gcatccggaa aaggcgcatc
     3241 gccccgatca gccgatcgag cgcaagccgc cctggatccg cgtgaaggcg cctggatcgc
     3301 ggcaattcgc cgagaccagc cgcattgttc gcacgaacaa gctggtgacg gtctgcgagg
     3361 aggcggcctg tcccaatatt ggcgaatgct gggacaagaa gcacgcgacc ttcatgatca
     3421 tgggcgatgt gtgcacccgc gcctgcgcct tttgcaatgt ccgcaccggc cttcccggag
     3481 cgctcgaagc gggcgaaccg gagcgcgtcg ccgaggcggt cgccaagctc ggcctcgccc
     3541 atgtcgtggt cacctcggtc gaccgcgacg atctcgccga cggcggcgcc gaacatttcg
     3601 cccgcacgat ccgcgccatc cgcgctgcgg ctcctgcgac cacgatcgag gtcctgacgc
     3661 cggatttcct ccgcaaggac ggagcgctcg aaattgtggt cggcgcgcgg ccggacgtgt
     3721 tcaaccacaa tctggagacg gttccgtcga aatatctgac ggtgcggccg ggcgcgcgtt
     3781 atttccactc ggtcaggctg ctccagcgcg tcaaagagat cgatccttcg atgttcacca
     3841 aatccggcat catggtcgga ctcggcgagg aacgtcatga agttctccag ctcatggatg
     3901 acctgcgctc cgcagaggtc gatttcctca ccatcggcca atatttgcag ccaagccgaa
     3961 agcatcaccg ggtcgtgcgc ttcgtgacgc cggacgagtt cgcctcctat gcggcgatcg
     4021 gcgcggcgaa gggattcctg ctcgtttcgt cgacgccgct gacgcggtcc tcgcatcatg
     4081 cgggcgacga tttcggccgg ctgaaggctg cgcgggtggc tcgttttgcg ccggccgccg
     4141 cttctattga cccgctggga gccaagctgt aggcaggccc cgcccttaga aaaagggagc
     4201 gcgtagctca gccggtagag catctgactt ttaatcagga ggtcatgggt tcgagtccca
     4261 tcgcgctcac caagcagatt ttaaagcccg gcagaggtca tttccgattg aagtggcgta
     4321 aagttgggcc agccagcccg cttccgcgac gcccttggaa gcccgtctgg tccgggaact
     4381 taagaggcgc aaggcggcca tgcggcgaaa tttgaggctc ttggcgcttc tcctggcgct
     4441 cggccaggct tgcccggctg tcgcgcaatt ctcgcccatc ccaagcatgc cgcaaccgat
     4501 cgtcgtcgtt ccgcccgccc agccgcaaaa gacctgctgc cgagacgagt gcgcgccgga
     4561 cgattcctgc cgccgcggcg cgacatgccc ggcgatctgc acgcgaaaat gcgcccgctg
     4621 tccgtgacgg cgcccggcaa gagcttccga aacgaacgcc aaggaaatta tggacttgga
     4681 cgcgccgcca tggtcttctt gcggccggct ggcgaccaaa cggagggtaa aacgtgcctt
     4741 cccggttcac ttcgggcgct tacgggccgg aactcgtgaa agtgatgacg ggcgcgttcg
     4801 atcgcgcgtg ggacgatttc gagccgcggc ctaggaattt gaacttagcc agatcgctga
     4861 tggcgagcgc gatcatcgag gcggtcgaag ccggcgatcg cgacgagcaa tcgctggttc
     4921 gcaaagcgac cgtcaccttg atcaaggcga tcaaggtcga cccggacctg ctcaacgccg
     4981 cggcgccgcc cgatctttcg aacgtttaac aacgttttgg cgcggagatt tgaacgccgg
     5041 atcggatcga ttgcggccga gccgaggtta tgaacatcgg cggcttggcg ttctcgccgt
     5101 cgcccgatac tgtccgctcg tcccaagccg attcgctcaa tcatggctcg tatcagcaaa
     5161 atttgcggct cgatcacgct cttcttcgtc atcgcgagcg gcggcgccgc gctggcggat
     5221 gttccgcggc ttcattgcgg cgtcctttcc cacccggtcg ccaatcttgg cgccgtcaat
     5281 ccgcgccatg cctattggtg caaatcgaat gtcgccggct atctcggcga ggaccgatcg
     5341 gttcctatcc gtccttacct cgctcatcga taccgcaggc gtcacagaaa acgcgtctgg
     5401 atcgactgat cagggcgtcg tgattcgaat ggcgcgacgc ggcgcgcctt taggcccatg
     5461 caaagcctcg gccgaccgcg tgtactcggc cgatagacgc gctttgtcga gagcgacaaa
     5521 atcggcagcg tgatgcggca gggccgcgcc gccctcaaaa accgccaagc cgttccgcgt
     5581 catcacgtaa tcgcctttgc gcagcgtcga atcctcggcc agcgaataat gcggcgcgat
     5641 gctgcgatgg cagacgcaag ctgcatccaa accggagcga tagcgcaagg cggtgggcaa
     5701 ttccgaatag cgctttccgg tctttgacac cgcttcgcca atatcgtcgg atccgttcgg
     5761 cgcctggtaa atcgccgtcg cggcgtccgg acaaagattc cggcattggg cttcttctcc
     5821 attccccgtc gagatcggaa aataggcgcc gtcgcaaagg cgcacgcaaa ccggatggcc
     5881 ggcgatcgcc gctatggagc ggcggcgcgg cgaggtcatg ggaatgcttg gcgccggcgc
     5941 ggtttgcggc gcgactttcg agtagttcgg cagcaaaagc tcatacggat tgccgatgtc
     6001 ggggcccctt gcgacgacgg tggtggcgac caggatcacg aacccggccg cgccgacgcc
     6061 ggcagcggcg gcgagcgatc gcctcttccc gttttcgaac ataacgcggc gctccgagcc
     6121 gagagaccgt cgcgataacc ggacgttccg cctttcgttc cgcgccgcgc tgatctgcgc
     6181 cgccggcgat tccgcttcgg cgcgcttgcc tgaccgcccg ccttggcctg ataaaagagc
     6241 gcgctttgca tgcggcgaag ccgacgcggg gcgcttttca ggggaggatc gatatgatgc
     6301 gcaagacggc tttggcggct gcgctcgttt tgggcggcgc ggcgacggcg ggcgcggcgc
     6361 ccgcgataaa ggtgaccagt ccggacatca agcccggcgc caaaatcgcc gaagaacagg
     6421 tcgccaatgg atttggctgc gagggcaaga acatttcgcc ggcgcttagc tggtcgggcg
     6481 cgccggccgg cgccaagagc ttcgcggtga cgatgtacga tcccgacgcg ccgaccggca
     6541 gcggcttctg gcactgggtc gttttcaata ttccggccgg
//
LOCUS       DATGJW010000033         3120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_938926, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000033 DATGJW010000000
VERSION     DATGJW010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3120
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_938926"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..740)
                     /locus_tag="VKU03_01015"
     CDS             complement(15..740)
                     /locus_tag="VKU03_01015"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ69864.1"
                     /translation="MTLIVKTVLADQIFEIVRARIISGALAGEAPIRQDALAAELGVS
                     KIPLREAFVRLEQDGLVVSKANRGFFVSPLSAAEAYDVFDLRLKLEPAAVAEGATRAG
                     EADRDAARRALAALDRATEARVASVASLNRAFHMALARPAGRRVTLMILERLQVVAER
                     YVVRHLEPSGRSARAIGEHQALYAAWAAGRAEQAARLSAMHIESTLRDLRTELEGAQF
                     SSPAKAGKGDHAKHGGGGRGLSG"
     gene            complement(897..1895)
                     /locus_tag="VKU03_01020"
     CDS             complement(897..1895)
                     /locus_tag="VKU03_01020"
                     /EC_number="5.1.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006327593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxyproline epimerase"
                     /protein_id="HLJ69865.1"
                     /translation="MRHVFFCIDGHTAGNPVRLVAGGAPLLRGVTMSERRQDFLARFD
                     WIRTGLCFEPRGHDMMSGGFLYPPTRDDADIGILFIETSGCLPMCGHGTIGLVTFGLE
                     HGLIQPRRPGRLRAEVPAGSIDLEYGREGDKVAWVKIVNVPAYVAARGVAIDVPGFGP
                     LSVDVAYGGNYYAIVEPQGSYKGLDDLGASRLVELSAKVRNLVRKSVEPVHPLDPTIR
                     GVSHVLWADKPKDARADGRNAVFYGERAIDRSPCGTGTSARLAHLAAQGRLRPGDRFV
                     HESYIGSLFTGRIEAETEIAGQKAIVPSIEGAAFATGFNTIWIDKAEPFWNGFQVR"
     gene            complement(2037..2951)
                     /locus_tag="VKU03_01025"
     CDS             complement(2037..2951)
                     /locus_tag="VKU03_01025"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018071799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HLJ69866.1"
                     /translation="MTTGWRGVFPAATTQFAEDLSVDIEATQRSLDALVKDGVHGLIL
                     LGTCGENNSLDPEEKRALLKAAVEAIGRRVPVVVGVSEFDARRAAAFARDAEKLGADG
                     LMVLPAMVYVPKPEELVAHFRAVAAATALPIMLYNNPPAYRVNIGVDVLKALQDVANI
                     KAVKESAPDPRRFTDLINAFGDRFDLFAGLDDVALEGLLLGAKGWVSGLTSAFPQESV
                     EMVAAAERGDWETARRIYRWFMPLLHLDAEHDLVQSIKLAEEIMGRGSERVRLPRLPL
                     SGARRVEVIAMVERAAATRPKLGLRDAA"
BASE COUNT          549 a         1029 c         1017 g          525 t
ORIGIN      
        1 acggcggttg agaattatcc tgagaggccg cggccccctc caccatgctt cgcatggtcc
       61 ccctttcccg ctttcgcggg ggaggaaaat tgcgcccctt ccaactcggt tcgtaaatct
      121 cgtaacgtac tttcgatatg catcgcgctg agcctagccg cctgctcggc cctgcccgcc
      181 gcccaggccg cataaagcgc ctggtgctcg ccgatcgcgc gcgcgctgcg gccgctcggc
      241 tcgagatgcc tgaccacgta acgctcggcg accacttgca ggcgttctaa gatcatcaag
      301 gtgacgcggc ggccggcggg gcgggcgagc gccatgtgga aagcgcggtt caacgacgca
      361 accgatgcga cgcgcgcttc ggtcgcgcgg tcgagcgccg cgagcgcccg ccgcgccgcg
      421 tcgcgatcgg cctccccggc ccgcgtcgcg ccttcggcga ccgccgccgg ctccagcttc
      481 agccgcaagt cgaacacgtc gtacgcttct gcggccgaga gcggcgagac gaaaaagccg
      541 cgattggcct tcgagacgac gagcccgtcc tgctccaatc tgacgaaggc ttcgcgcaag
      601 gggattttgc tgacgccgag ctcggcggcg agcgcgtcct ggcggatcgg cgcctcgccc
      661 gcgagcgcgc cgcttatgat gcgggcgcgg acgatctcga aaatctggtc ggcgagaaca
      721 gttttaacga tgagggtcat gaagacgcgc cgactttcga atggattgct tcgtcgcttg
      781 gctcctcgca atgacggact gcgctctcgc cattcccgag aaagcggaaa tccatgaggc
      841 cgccgccctt gcgagccggc gcagcctgga ttgccgcttg cgcggggttg cgctcctcac
      901 cgcacctgaa acccgttcca gaacggctcc gccttatcga tccagatcgt gttgaatccg
      961 gtcgcgaaag ccgcgccttc gatcgacgga acgatcgcct tctggccggc gatctccgtc
     1021 tccgcctcga tccgcccggt gaacaagctg ccgatatagc tttcgtgaac gaagcggtcg
     1081 cccggccgca ggcggccttg cgcggccaga tgagcaaggc gcgcggaagt gcccgtcccg
     1141 cagggcgaac ggtcgatggc gcgctcgcca tagaagaccg cgttgcggcc gtcggcgcgc
     1201 gcatctttcg gcttgtcggc ccagagcaca tgcgagacgc cgcggatcgt cggatcgagc
     1261 ggatggaccg gctcgacgct cttgcgcacg aggttgcgta cttttgcgct aagttcgaca
     1321 agccgacttg cgccgagatc gtcgaggcct ttgtaggagc cttgcggctc gacgatcgca
     1381 tagtagttgc cgccataagc gacatcgacg gagagcggac cgaagccagg tacgtcgatg
     1441 gcgacgccgc gcgctgcgac ataggccggc acattgacga ttttgaccca ggcgaccttg
     1501 tcgccctccc ggccatattc gagatcgatc gacccggccg ggacttcggc gcgcagccgg
     1561 ccgggccggc gcggctggat caagccatgc tccagcccga acgtgacaag accgatcgtt
     1621 ccgtgcccgc acatcggcag gcagccgctc gtctcgatga agagaatgcc gatatcggcg
     1681 tcatcgcgcg tcggcggata gagaaagccg cccgacatca tgtcgtggcc gcgcggctcg
     1741 aagcagaggc cggtcctgat ccaatcgaag cgggcgagga aatcctggcg gcgctccgac
     1801 atggtgacgc cgcgaagaag cggggcgccg cccgcgacca ggcgcaccgg attgccggcg
     1861 gtgtggccgt cgatgcagaa gaagacgtgg cgcatgggat cgactttaaa tgtagcgcgg
     1921 cgggtatttg attcaccgtc atttgcgagg agcgaagcga cgaagcaatc cattcgtagg
     1981 gcggcgctct atgaaccgat tgcctcgctt cgctcgcgat gacggctggg acggtttcac
     2041 gccgcgtcgc gcaggccgag cttcggccgc gtcgcggcgg ctcgctcgac catcgcgatc
     2101 acctcgacgc ggcgcgcgcc ggaaagcggc aggcgcggca ggcgcacgcg ctccgagccg
     2161 cggcccatga tctcttcggc gagcttgatc gactggacga gatcgtgctc ggcgtcgaga
     2221 tggagcaagg gcatgaacca gcggtagatg cgccgcgccg tctcccaatc gccgcgctca
     2281 gccgccgcga ccatttcgac cgattcttgc ggaaaggcgc tggtcaggcc tgagacccag
     2341 cccttggcgc cgagcaacag cccttccagc gcgacatcgt cgagcccggc gaagagatcg
     2401 aaacggtcgc cgaacgcgtt gatgagatcg gtgaagcggc gcggatcggg cgcgctctcc
     2461 ttgaccgcct tgatgttcgc aacgtcctgc agagccttaa ggacatcgac gccgatattc
     2521 acccggtagg ccggcggatt gttgtagagc atgatcggca aggccgtcgc cgcggcgacg
     2581 gcgcggaaat gcgcgacgag ctcctcaggc ttcggcacat agaccatggc cggcaggacc
     2641 atcaatccgt cggcgccgag cttttcggcg tcgcgggcga aggccgcggc gcggcgcgca
     2701 tcgaattcgg aaaccccgac cacgaccgga acgcgacgtc cgatcgcttc gaccgccgcc
     2761 ttgagcagcg cgcgcttctc ctcgggatcg agcgaattgt tctcgccgca tgtgccgagc
     2821 aggatcaggc cgtggacgcc gtctttcacc agcgcgtcca gactgcgctg ggtcgcttcg
     2881 atgtctacgc tcaaatcctc ggcgaattgc gtcgtcgccg ctggaaacac gccgcgccag
     2941 ccggtggtca tgacaatctc caaaatcgta tacgatatac gatgccagtt aggctcgaat
     3001 gtcaagcgcg gcaaagcgcc cgatgccgcg cccacgattt gagttagatt actggtcgga
     3061 attggaggct cgttcatgtc cgaagacagc ttggtcgtcg acgatcgcca gcttgcgccc
//
LOCUS       DATGJW010000034         3497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1042762, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000034 DATGJW010000000
VERSION     DATGJW010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3497
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1042762"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..720)
                     /gene="cdd"
                     /locus_tag="VKU03_01030"
     CDS             complement(304..720)
                     /gene="cdd"
                     /locus_tag="VKU03_01030"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019902947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HLJ69867.1"
                     /translation="MSKSADPSVLAALFEAAKAAQSKAYAPYSRFRVGAAILTPSGAI
                     HAGCNVENAAYPQGACAEANAIGAMVQGGEQRIAEILVIGDGDALVTPCGGCRQRIRE
                     FAALDAPIHIAGPDGVRATFTLEELLPHSFGPDHLK"
     gene            complement(704..1675)
                     /locus_tag="VKU03_01035"
     CDS             complement(704..1675)
                     /locus_tag="VKU03_01035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003536145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ69868.1"
                     /translation="MDAFDAFIVIFASGIRLAVPLILGCLAGLWSERSGIVDVGLEGK
                     MLTGAFAAAAAATFTGSAWIGLCAGVVATVLMSLIHGYASIDQRANQIVSGVAINMVA
                     DGMTALIGNAWWGQGGRTPQLPDSALFNAITLPDAQSFGRVPIIGPIYQHLLSGHDLI
                     TYAAFVLAPATYWVLYKTRFGLRLRAVGENPAAVDTAGISVRALRYGGVIICGVLVGL
                     AGTYLSIAQAAYFQPHMSAGKGFIALAALVFAKWRPMPALATCLLFGFLDALSIRLQG
                     TILPVIGPVPVQAIQALPYILTVILLAGFIGKAIPPKASGIPYVKER"
     gene            complement(1888..3015)
                     /locus_tag="VKU03_01040"
     CDS             complement(1888..3015)
                     /locus_tag="VKU03_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011582962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ69869.1"
                     /translation="MSQPADLPKWADVAAIPLINVAAAFVISGLVVTAIGENPFEAVK
                     LLIVGAIGDLEGIGFTLYYATSFIFTGLAVAICFQAGLFNIGVDGQAYVAGLGAALVA
                     LHFGFLPGVLLAPLAVLGAALFGAGFAAIPGYLQAKRDSHLVITTIMFNYIASALMGY
                     LLVDVLRNPGQMNPETPGFPPGAAMPQISDLVAALGWQWPNTPFNLSFVLALLCAWLI
                     WLLIWRTRLGYEIRAVGANPTAAIYGGVSPARITMITTLLSGALAGGLAVNVLLGEEH
                     RLILQYTADFGFVGIAVALMGRGHPAGIVLAAILFGMLYQGGAELAFDKPKITRDMIV
                     VIQGLVVLFAGALEHMFRRPLALFLARFQAPVADAPAAERA"
     gene            complement(3012..>3497)
                     /locus_tag="VKU03_01045"
     CDS             complement(3012..>3497)
                     /locus_tag="VKU03_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC transporter ATP-binding protein"
                     /protein_id="HLJ69870.1"
                     /translation="VMPFQACESAMLGFQDDKVYGAGLFFDRGAIVADEAEKMEAFDI
                     RPRAPLLKTANFSGGNQQKIVLAREIERNPHVMLVGQPTRGVDIGAIEFIHKRIVALR
                     DSGKAVLLVSVELDEIMALSDRILVVCGGRITGERLPETCNQQDLGLLMAGVSEREHA
                     A"
BASE COUNT          689 a         1122 c         1191 g          495 t
ORIGIN      
        1 aatccgcgcc gcctgccgaa gcgccaagtc gacaagagcc gctttgtcca gcgaatcaag
       61 atatgcgcga ttcatcctta tcgtgaatca cgccgcccca cgccgcgcaa gcttaaagcc
      121 gataaggcgc taaacagata caagcgggga gaggtaaggt gaggggctgg tcggtcaatc
      181 gcaaccgtcg gctttaccgc ccccggcccc tctccgtacc tctccccgcg aagcggggag
      241 aggggtcctc ggcgtcgcgc cctatatctc atcccgtgta ccggctgccg attcagcacg
      301 cgatcacttc aaatgatccg gcccgaacga atgcggcagc aattcttcga gcgtgaaagt
      361 cgcgcgcacg ccgtccggcc cggcgatatg gatcggcgcg tcgagggcgg cgaattcgcg
      421 gatgcgctgg cggcagccgc cgcacggcgt caccagggcg tcgccgtcgc cgatgaccag
      481 gatttccgcg atgcgctgct cgccgccttg gaccatggcg ccgatcgcgt tcgcctcggc
      541 gcaggcgcct tgcggatagg cggcgttctc gacgttgcag ccggcatgga tcgcgccgga
      601 cggcgtcagg atcgccgcgc cgacgcgaaa gcgcgaatag ggcgcatagg ctttgctctg
      661 cgccgccttg gccgcctcga acaacgcggc caaaacggag ggatcagcgc tctttgacat
      721 aggggattcc cgaagccttg ggcgggatcg ccttgccgat gaagccggcg agcaggatca
      781 ccgtcaggat atagggcaaa gcctggatcg cctggaccgg caccggcccg atcaccggca
      841 ggatcgtgcc ttgcaggcgg atcgacaagg cgtcgaggaa gccgaacaag aggcaggtgg
      901 cgagagccgg catcggccgc catttggcga agaccagagc ggcgagggcg atgaagccct
      961 tgccggcgct catgtgcggc tggaaatagg cggcctgcgc gatcgaaagg tacgtcccgg
     1021 cgaggccgac aaggacgccg cagatgatca cgccgccata gcgcaaggcg cgcaccgaaa
     1081 tgccggccgt gtcgaccgcg gcgggatttt cgccgaccgc cctgaggcgc agcccgaacc
     1141 gggtcttata gagaacccaa taggtcgcag gcgccagaac gaacgccgcg taggtgatca
     1201 ggtcgtgccc cgaaagcagg tgctgataaa tcggaccgat gatcgggacc ctcccgaagc
     1261 tctgcgcgtc gggaagcgtg atcgcgttga acagagccga atccggcaat tgcggcgtgc
     1321 ggccgccctg gccccaccag gcgttgccga tcaaggcggt catgccgtcg gcgaccatgt
     1381 tgatggcgac gccggagacg atctggttgg cgcgctgatc gatcgaggcg tagccatgga
     1441 tgagggacat cagcacggtc gccacgacgc ccgcgcaaag cccgatccag gcgctgccgg
     1501 tgaaggtcgc cgcggcggcc gcggcgaagg cgccggtcag catcttgcct tcgaggccga
     1561 catcgacaat gccggagcgc tccgaccaca ggccggcgag gcagccgagg atcagcggca
     1621 cggcgaggcg aatgccggag gcgaagatga caatgaaggc gtcgaaagcg tccattgctc
     1681 tatgcgaccg tggggtggga gcacaaacga tcggcgccgc ggcttaccct caccccagcc
     1741 ctctcccgca agcgggagag ggggcgcgcc gcggcgtgct gtagccgcgg tcgccgaccg
     1801 cggctacaac cccttctccc gcttgcggga gaaggtggcg cgaagcgccg gatgagggtg
     1861 aggcgcaagg cctgccagtt cagcatgtca cgccctctcg gcggcgggcg cgtcggcgac
     1921 cggcgcctgg aagcgggcca ggaacaaagc cagcggccgc cggaacatgt gctccagcgc
     1981 gccggcgaag agcacgacca gaccctggat caccacgatc atgtcgcggg tgattttcgg
     2041 cttgtcgaag gcgagctcgg cgccgccttg gtacagcatg ccgaagagaa ttgcggcgag
     2101 cacgatgccg gccggatggc cgcgccccat cagcgccacg gcgatgccga cgaaaccgaa
     2161 atcggccgtg tattgcagga tgaggcgatg ctcctcgccg agcagcacgt tgacggcgag
     2221 gccgccggcc agcgccccgg acaggagcgt cgtgatcatg gtgatgcggg cgggcgagac
     2281 gccgccatag atcgccgcgg tcggattggc gccgacggcg cgaatctcgt agccgagtcg
     2341 cgtccgccag atcagaagcc agatcagcca ggcgcaaagc aaagccaaaa cgaagctcag
     2401 attgaacggc gtgttcggcc attgccagcc gagagcggcg acgaggtcgc tgatctgcgg
     2461 catggccgcg ccgggcggaa agccgggcgt ctccggattc atctggccgg gattacgcaa
     2521 aacgtcgacg agcagatagc ccatcagcgc cgaggcgatg tagttgaaca tgatcgtcgt
     2581 gatgacgaga tggctgtcgc gcttggcttg cagatagccg ggaatcgccg caaagcccgc
     2641 gccgaacaga gccgcgccga gcaccgcgag cggcgccagc aggacgcccg gcagaaagcc
     2701 gaaatgcagc gccaccaaag ccgcgccgag gccggcgaca taggcttggc cgtcgacgcc
     2761 gatattgaac aacccggcct ggaagcagat cgcgacggca aggccggtga agatgaagct
     2821 cgtcgcataa tacagcgtga agccgatccc ttcgagatcg ccgatcgcgc cgacgatcaa
     2881 aagcttgacc gcctcaaacg gattttcgcc gatcgcggtc acgaccaggc cggaaatgac
     2941 gaaagcggcg gcgacgttga tcagcgggat cgccgcgaca tcggcccatt tgggcaaatc
     3001 ggccggctgg ctcatgcggc gtgctcgcgc tcggaaacgc cggccatcag caggccgaga
     3061 tcctgctggt tgcacgtctc ggggagccgc tcgccggtga tgcgtccgcc gcaaacgacg
     3121 aggatgcggt ccgacaaagc catgatctcg tcgagttcga cggagacgag cagcaccgcc
     3181 ttgccggaat cgcgcaaggc gacgatgcgc ttatggatga attcgatcgc gccgatatcg
     3241 acgccgcgcg tcggctggcc gaccagcatg acatgcggat tgcgctcgat ctcgcgcgcc
     3301 agcacgatct tctgctggtt gccgccggag aaattcgccg tcttgagcag cggcgcgcgc
     3361 ggccggatgt cgaaggcctc catcttctcg gcctcgtcgg cgacgatcgc gccgcggtcg
     3421 aagaacaagc ccgccccata gaccttgtcg tcctggaaac cgagcatcgc gctctcgcaa
     3481 gcctggaacg gcatgac
//
LOCUS       DATGJW010000035         6776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_524180, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000035 DATGJW010000000
VERSION     DATGJW010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6776
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_524180"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..233
                     /locus_tag="VKU03_01050"
     CDS             <1..233
                     /locus_tag="VKU03_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69871.1"
                     /translation="PDCEKYKEPLAYNRCLASHGPSAARALAARASEGAASEDHRFHS
                     RSIRHSHRGRMSATFVIREGHASHRRGKFSQM"
     gene            437..754
                     /locus_tag="VKU03_01055"
     CDS             437..754
                     /locus_tag="VKU03_01055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008552324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2849 domain-containing protein"
                     /protein_id="HLJ69872.1"
                     /translation="MSEVITANRLIDGIVVFQAENGWVEDFAQATIYDNAEASKQALA
                     RAKEDEARDVVVDSYAIVVEMRSGHYAPKALREIIRAAGPTTRPDLGKQAQGQSPRFV
                     EGR"
     gene            766..2439
                     /locus_tag="VKU03_01060"
     CDS             766..2439
                     /locus_tag="VKU03_01060"
                     /EC_number="1.8.7.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0050311 - sulfite reductase (ferredoxin)
                     activity [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite/sulfite reductase"
                     /protein_id="HLJ69873.1"
                     /translation="MYRYDEFDAAFVAQRVNQFRDQVDRRVKGELTEDQFRPHRLMNG
                     LYLQLHAYMLRIAVPYGTLASRQMRKLAEIARKYDRGYGHFTTRQNLQFHWPALKDVP
                     DILAELASVEMHSIQTSGNCIRNVTADHFAGATADEIADPRPYAEIIRQWSSLHPEFS
                     FLPRKFKIAVNAAPKDRAAIQAHDIGLQIVRNDEGEIGFTVYVGGGLGRTPMIAHKVR
                     DFLPEADLLAYIAAILRVYNLEGRRDNKFKARVKILVHEKGEAAMREAVEQEFAAGAR
                     DGLELPRAEIERIRAYFALPALEAKKPVSERCERRRGKDAKFSSFMQRNVAPHKVPGY
                     AIVTISLKPIGGTPGDCSADQMDAVADLAEDYSADELRVSHEQNLVLPHVALDDLPAV
                     YDRLASVGLATPNAGLISDIISCPGLDYCTLATARSIPIAQRISQRFGDGARAREIGP
                     LKIKISGCINACGHHHVGHIGILGLERAGEETYQITLGGSGDETASLGQITGPGFSTE
                     TVVDAVETIVSTYLGLRRDPSEDFLAAFRRVGMAPFKEALYERSAGSDI"
     gene            2442..3179
                     /locus_tag="VKU03_01065"
     CDS             2442..3179
                     /locus_tag="VKU03_01065"
                     /EC_number="1.8.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004604 - phosphoadenylyl-sulfate
                     reductase (thioredoxin) activity [Evidence IEA];
                     GO_process: GO:0019379 - sulfate assimilation,
                     phosphoadenylyl sulfate reduction by
                     phosphoadenylyl-sulfate reductase (thioredoxin) [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoadenylyl-sulfate reductase"
                     /protein_id="HLJ69874.1"
                     /translation="MSAHDIKLFGDQIAASRIEVKFAGLKAASLLRLAIQELFPGRIA
                     LVSSFGADSAVLLHMVSEIDKATPVVFVDTGQLFPETLAYRDALCATLGLTNVIVATP
                     NEAVLAKEDPEKFLFADDPDRCCDIRKVLPLAEALEGYEAWITGRKSFQSVTRATLPL
                     FEAEGERVKVNPLVGWSAGDILDYIKRAGLPPHPLVAKGYPSIGCLPCTTPVRPGEDG
                     RAGRWRGRGKIECGIHIGALSSAGSDI"
     gene            3181..3687
                     /locus_tag="VKU03_01070"
     CDS             3181..3687
                     /locus_tag="VKU03_01070"
                     /inference="COORDINATES: protein motif:HMM:NF017849.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF934 domain-containing protein"
                     /protein_id="HLJ69875.1"
                     /translation="MALWRRDGFADNIWTRVADGDALPAAGAIIVGVGRWRAEREALS
                     ARADPVGLAVSPGKEAYEHLREAAERPLVALDFPKFADGRAFSLAQLLRQRFGFKGEL
                     RAIGDVLLDGLALMLRCGFDSFEIVNEPTLRALERGWKPPVSLYYQPSLVREAPAGTR
                     PWLRRAAG"
     gene            complement(3784..4719)
                     /locus_tag="VKU03_01075"
     CDS             complement(3784..4719)
                     /locus_tag="VKU03_01075"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ69876.1"
                     /translation="MVQVAFLGLGVMGYPMAGHLQAKGHRTTVYNRTAAKAARWVAQH
                     GGASASSPREAAADCDFVFACVGNDDDLRSVALGPDGALAGMKPGSVFVDHTTASAFV
                     ARELHAEAGKRGLCFIDAPVSGGQAGAENGALTVMCGGEAEPYGRAEPVIAAYARACR
                     LLGGPGAGQLTKMVNQICIAGLVQGLAEGLQFARKAGLDLEAVIAVISKGAAQSWQMD
                     NRSKTMAAGKFDFGFAVDWMRKDLGICLDEARRNGAPLPVTALVDQFYSDVQARGGGR
                     WDTSSLIARLETPRPAQRGEGGREAAGCKGQRRTL"
     gene            complement(4798..5403)
                     /gene="mobA"
                     /locus_tag="VKU03_01080"
     CDS             complement(4798..5403)
                     /gene="mobA"
                     /locus_tag="VKU03_01080"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase MobA"
                     /protein_id="HLJ69877.1"
                     /translation="MPRPRMMLGVVLAGGKSTRMGAEKAFAPLENRPLIAYAIERLRP
                     QVQALAISANGDPRRFAAFGLPVLADPPSESDAGPLAGVAAGLAYAAREGHDIVATAP
                     CDAPFAPTDFVRRLAEAMIDSAAPAALVESPRGLEPLFALWRVDLLAALRAHLGGGGR
                     SPRDFLLAQGAARARFADEEPFANLNSPAEMARARAKAKPG"
     gene            5522..6646
                     /locus_tag="VKU03_01085"
     CDS             5522..6646
                     /locus_tag="VKU03_01085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ69878.1"
                     /translation="MSAGRRQFIAGLGLATAGLAAAKPALADSAPEVHWRLTSSFQPS
                     LDLIYGGAETFAATLSDLTDGRFTVTVLPAGEIAGALDALDAASNGVAECAHTALHYY
                     WNKDPTFVFGSSTPFGMNARQHTAWLMEGGGGQLLDAFLADHKVFALPAGNTGGQMAG
                     WFRKEVKTVNDLQGMKVRIGGFAGKVFQLLGAEPVAMPKDSVYASFESGALDAMEWVG
                     PYDDDKFGERKDGEKQVLAKVAPYYYYPGWWRGGMQLHLIVNRQKFDALPKPYQAALR
                     AAASVANATMQAKYDAANPAALKRLVVGGAELRLFPQDVLEACYKTASDLYSQLSAEN
                     ANFKKIADSYMAFRGDQYLWWQVAEYSFDNFMIRQRRTKS"
BASE COUNT         1178 a         2295 c         2166 g         1137 t
ORIGIN      
        1 accccgattg cgaaaaatat aaagagccgc tcgcctacaa tcggtgcctg gcctcgcatg
       61 gtccgtcggc cgcccgcgct ttggccgcgc gggccagcga gggggcggcg tcggaagacc
      121 atagatttca cagccgatcg atccgccatt cccatcgcgg ccgaatgagc gcgactttcg
      181 tgatccgtga gggccatgcc tcgcatcggc gcggcaagtt ctcgcaaatg tgagaattcc
      241 cgccattgaa ctaaaaattt ttgttataat aattatatcc tagattttct tcgtatattc
      301 tgctatccgc ttcttcagac aagcaaatct cgcattttgg atacgtgaat tgagcaggcg
      361 agacgatgcg agcgccttga attccgccct gcgacaaaca tctatcgccg acgcgtccgg
      421 agccgtttga ccagccatga gcgaagtcat caccgccaat cgcctgattg acggcatcgt
      481 cgtcttccaa gccgagaacg gctgggtcga agacttcgcc caagcgacga tttacgacaa
      541 cgcggaggcg agcaaacaag cgctggcccg cgccaaagaa gacgaagcgc gcgacgtcgt
      601 cgtcgattcc tacgcgatcg tggtcgagat gcgcagcggc cattatgcgc cgaaagcgct
      661 tcgcgaaatc attcgcgccg ccggtccgac cacccggccc gatctcggca agcaagcgca
      721 ggggcaatct ccccgctttg tcgaaggacg ctgacggagc tcccgatgta ccgttatgac
      781 gaattcgacg ccgccttcgt cgcccagcgc gtcaatcagt tccgcgatca ggtcgaccgt
      841 cgcgtcaaag gcgaattgac cgaggaccag ttccggccgc accggctgat gaacggcctc
      901 tatctgcaac tgcacgccta catgctgcgc atcgcagtgc cctatggcac gctggcttcg
      961 cgccagatgc gcaagctcgc cgagatcgcc cgcaaatacg accgcggcta cggccatttc
     1021 acgacgcggc agaatctgca gttccattgg ccggctttga aggacgtgcc ggacattctc
     1081 gccgagctcg ccagcgtcga gatgcattcg atccagacct ccggcaattg catccgcaac
     1141 gtgaccgccg accatttcgc cggcgccacc gccgacgaga tcgccgaccc gcgcccctat
     1201 gccgagatca tccggcaatg gtcctctctc catccggaat tttcgttcct gccgcgcaag
     1261 ttcaagatcg cggtcaacgc cgcgccgaaa gatcgcgccg cgatccaggc gcacgatatc
     1321 ggcctgcaga tcgtgcgcaa cgacgaagga gagatcggct ttacggttta tgtcggcggc
     1381 ggcctcggcc gcacgccgat gatcgcgcac aaagtgcgcg acttcctgcc cgaggccgat
     1441 cttctcgcct atatcgcggc gatcttgcgg gtctacaatc tggagggccg gcgcgacaac
     1501 aagttcaagg cgcgcgtcaa gatcctcgtc cacgagaagg gcgaggcggc gatgcgcgag
     1561 gcggtggagc aggaattcgc cgccggcgcg cgcgacgggc tcgaattgcc ccgcgcggag
     1621 atcgagcgca tccgcgccta tttcgccctg ccggccttgg aggcgaagaa gccggtcagc
     1681 gagcgctgcg agcgccggcg cggcaaggac gccaagtttt cgagcttcat gcagcgcaac
     1741 gtcgcgccgc ataaagttcc gggctacgcc atcgtcacga tttcgctcaa gccgatcggc
     1801 ggcacgccgg gcgattgcag cgccgaccag atggacgcgg tcgccgatct cgccgaggac
     1861 tattccgccg acgaactgcg cgtctcgcac gagcagaacc tggttctgcc gcatgtcgcg
     1921 ctcgacgatc tgcccgccgt ctacgatcgg ctcgcctcgg tcggcctggc gacgccgaac
     1981 gccggcctga tcagcgacat catttcctgc cccggccttg attattgcac gctcgccacg
     2041 gcccgctcga tccccatcgc ccagcggatt tcgcagcgct tcggcgacgg cgcgcgcgcc
     2101 cgggagatcg gcccgctcaa gatcaagatt tccggctgca tcaacgcctg cggccatcac
     2161 catgtcggcc atatcggcat tctcggcctg gagcgcgccg gcgaagagac gtaccagatc
     2221 acgcttggcg gctcgggcga cgagaccgct tccctcggcc agatcaccgg gccgggcttt
     2281 tcgaccgaga cggtcgtcga cgcggtcgag acgatcgtct cgacctatct cgggttgcgc
     2341 cgcgatccga gcgaagactt cctcgccgcc ttccgccgcg tcggcatggc gccgttcaaa
     2401 gaggcgctct acgagcgcag cgccggatcg gacatttgac catgagcgcg cacgacatca
     2461 agcttttcgg cgaccagatt gccgcctcgc gcatcgaggt gaaattcgcc ggcctgaaag
     2521 cagcgtcgct gctgcggctg gcgatccagg aattgtttcc cggccggatc gcgctcgtct
     2581 ccagcttcgg cgccgattcg gccgtgctgt tgcacatggt gagcgagatc gacaaggcga
     2641 cgccggtcgt gttcgtcgac accggccagc tctttccgga gaccttggcc tatcgcgacg
     2701 cgctttgcgc cacgctcggc ttgaccaatg tgattgtcgc aacgccgaac gaggcggttc
     2761 tggccaagga ggaccccgag aaattcctct tcgccgacga tcccgaccgc tgctgcgaca
     2821 ttcgcaaggt gctgccgctc gccgaggcgc tcgaagggta cgaagcctgg atcaccggcc
     2881 gcaagagctt ccagagcgtg acccgggcga ccttgccgct gttcgaggcc gaaggcgagc
     2941 gcgtcaaggt caatcccctg gtcggctgga gcgccggcga cattctcgac tacatcaagc
     3001 gcgccgggct gccgccgcat ccactcgtcg ccaaaggcta tccgtcgatc ggctgcctcc
     3061 cctgcaccac gccggtcagg cccggcgagg atgggcgcgc cggccgctgg cgcggccgcg
     3121 gcaaaatcga atgcggcatt catatcggcg cgctctccag cgccggatcg gatatctgac
     3181 atggccctgt ggcgacgaga cggcttcgcc gacaacatct ggaccagggt cgccgacggc
     3241 gacgccttgc cggccgccgg cgcgatcatc gtcggcgtcg gccgctggcg ggccgaacgc
     3301 gaggcgctga gcgcgcgggc cgatccggtc ggcctcgccg tctcccccgg caaggaggct
     3361 tacgagcatt tgcgcgaagc cgccgagcgg ccgctggtcg cgctggactt tccgaaattc
     3421 gccgatggcc gcgccttctc gctcgcccaa ttgctgcgtc agcgcttcgg cttcaaaggc
     3481 gagttgcgcg cgatcggcga cgtgctgctc gacggcttgg cgctgatgct gcgctgcggc
     3541 ttcgacagtt tcgaaatcgt caacgagccg actttgcgtg cgcttgagcg cggctggaag
     3601 ccgccggtgt cgctctacta tcagccgagc ctggtccgcg aagcgccggc cgggacgcgg
     3661 ccgtggctca ggcgggcggc ggggtgagta tctcttctcc ccgcaaggcg gggtgaaggt
     3721 ggcgcgaagc gccggatgag gggcggcgca ggactcgaaa gtacgtgcgc tttgaattgc
     3781 taatcacaag gttcggcgct gccccttgca cccggccgct tcgcggccac cctctccccg
     3841 ctgcgcgggg cgaggggttt ccagccgcgc gatcaggctc gacgtatccc agcggccgcc
     3901 gcccctcgcc tgcacgtccg aatagaattg atcgaccaag gccgtaaccg gcagaggcgc
     3961 gccgttgcgc cgcgcctcat cgaggcaaat gccgaggtcc ttgcgcatcc aatcgacggc
     4021 gaagccgaaa tcgaacttgc cggcggccat ggtcttggag cgattgtcca tctgccagga
     4081 ttgggccgcg cctttcgaaa tcacggcgat caccgcctcc agatcgagcc cggccttgcg
     4141 ggcgaattgc aggccctcgg cgaggccttg gacgaggccg gcaatgcaaa tctggttgac
     4201 cattttcgtc aattgcccgg cgcccgggcc gcccagaagc cggcaggcgc gggcgtaagc
     4261 cgcgatgacc ggctcggcgc ggccgtaagg ctccgcctcg ccgccgcaca tcaccgtcaa
     4321 ggcgccgttc tcggcgcccg cctgtccgcc cgagaccggc gcgtcgataa agcaaaggcc
     4381 gcgcttcccg gcctccgcgt gcagctctcg cgcgacaaac gccgaagcgg tggtgtgatc
     4441 gacgaagacc gagccgggct tcatgccggc caaagcgccg tcaggcccaa gcgcgacgct
     4501 gcgcagatcg tcgtcattgc cgacgcaggc gaagacgaaa tcgcaatcgg ccgccgcctc
     4561 gcgcgggctc gacgcgctgg cgccgccatg ctgggcgacc cagcgcgcgg ctttcgccgc
     4621 cgtacggtta tagaccgtgg tccgatggcc cttggcctga agatgtccgg ccatcggata
     4681 gcccatcacg cccaaaccga ggaatgcgac ctgaaccaaa ggaaactccg ttaaattcgc
     4741 ccgcgcgcga aggttgcctt aacgccttcg aagcgacaag gcgaggcgga tcgccggtta
     4801 gcccggcttg gccttggcgc gggcgcgcgc catctccgcg gggctgttga gattggcgaa
     4861 aggctcttcg tcggcgaaac gcgcccgcgc cgcgccttgg gcgagcagga agtcgcgcgg
     4921 gctgcgcccg ccgccgccga gatgagcgcg taaggcggcg aggaggtcca cccgccagag
     4981 cgcgaagagc ggctcaaggc cgcgcgggct ttcaacgagc gcggccggcg ccgccgaatc
     5041 gatcatggcc tcggccaaac gccggacgaa atcggtcggc gcaaaaggcg cgtcgcaggg
     5101 cgctgtggcg acaatgtcgt ggccctcgcg cgccgcatag gcgaggccag ccgcgacgcc
     5161 tgcaagcggg ccggcgtcgc tttcggatgg ggggtcggcc agcaccggca ggccgaaggc
     5221 ggcgaagcgg cgcggatcgc cattggcgct tatggccaga gcttggacct gcgggcgcaa
     5281 gcgctcgatc gcatacgcaa tgaggggacg gttctccaac ggcgcaaagg ccttctcggc
     5341 gcccatccgc gtcgatttcc cgccggcgag aacaacgccg agcatcatgc gcggacgcgg
     5401 caagcgatct ccttttttcc cgcgccggcg gcgtcgccgc ttcgcgtttg aatcgcgccg
     5461 caatttggcg ccggatcgcc tatatagtga tcggcgttat tctcggccac ggatggagtt
     5521 catgagcgct gggcgtcgtc aattcatcgc cggattgggc ctcgcgacgg ccgggctcgc
     5581 ggcggccaag ccggccctcg ccgattcggc gccggaagtc cattggcgcc tgacgtcgag
     5641 ctttcagccg agcctcgatc tcatctatgg cggcgcggag actttcgccg ccaccctttc
     5701 ggacctgacc gacggccgct tcacggtcac ggtgctgccg gccggcgaga tcgccggcgc
     5761 tctcgacgcg ctcgacgccg cgtcgaacgg cgtcgccgaa tgcgctcaca ccgccctgca
     5821 ctattactgg aacaaggatc cgaccttcgt cttcggctcc tcgacgccgt tcggcatgaa
     5881 tgcgcgccag cataccgcct ggctcatgga aggcggcggc ggccaattgc tcgacgcgtt
     5941 tctcgccgat cacaaagtgt tcgcgctgcc ggccggcaat accggcggac agatggcggg
     6001 ctggttccgc aaagaggtga agacggtcaa cgatctgcaa gggatgaagg tgcggatcgg
     6061 cggtttcgcc ggcaaggtct tccaattgct cggcgccgag ccggtcgcca tgccgaagga
     6121 cagcgtctac gcgtcgttcg aatcgggcgc gctcgacgcg atggagtggg tcgggcctta
     6181 cgacgacgac aagttcggcg agcgcaagga cggcgagaag caggtcctcg cgaaagtcgc
     6241 gccctattac tattatccgg gctggtggcg gggcggcatg cagctccacc tcatcgtcaa
     6301 ccgccagaaa ttcgacgcgc tgcccaagcc ctaccaggcc gctttgcgcg ccgcggcgag
     6361 cgtcgccaac gccacgatgc aggccaaata cgacgcggcc aatcccgccg ccttgaagcg
     6421 cctcgtggtc ggcggcgccg agttgcggct ctttccgcag gacgtgctcg aagcctgcta
     6481 caagaccgcc agcgacctct acagccagct ctcggcggag aacgccaatt tcaagaagat
     6541 tgccgattcc tacatggcct tccgcggcga tcagtaccta tggtggcagg tggcggaata
     6601 ttcgttcgac aatttcatga tccggcagcg gcggaccaag agctgagcgc ggccttcccc
     6661 ttctccccgc ttcgcgcagg gctgtccggg gaaagtctga ggttatggcg cgtcttggac
     6721 gcaatctttc agcgccgcgg cgccacgccg gccgatcgcc gtcaacccgc catagc
//
LOCUS       DATGJW010000036         3270 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_109752, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000036 DATGJW010000000
VERSION     DATGJW010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3270)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3270)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3270
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109752"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            516..1508
                     /locus_tag="VKU03_01090"
     CDS             516..1508
                     /locus_tag="VKU03_01090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent oxidoreductase"
                     /protein_id="HLJ69879.1"
                     /translation="MPVNKQIVLDSRPAGAAEAGNFRLIESEAPNPGPGQVLVRHLYL
                     SLDPYMRGRMSDAKSYAKPQELGAVMVGGTVGEVVASNNPRFKAGDKVVGMGGWQNYA
                     LSDGGDLRVVDASRIPIQAYLGPLGMPGVTAWYGLNKIIAPKNGETVVVTAATGAVGS
                     VVGQLAKLAGATAIGVAGGPEKCAYARDELGYHVCIDHKSPSFVADLKAALPKGVDGL
                     FENVGGLPFETAARRFNDFARVAICGLIASYDGAPAPAIDLRVMLTKRIRIEGFIVSD
                     HMALWPTALAELAGHVAAGRIRYRETISQGLESAPEAFLGLLKGKNFGKQLVKL"
     gene            1618..2604
                     /locus_tag="VKU03_01095"
     CDS             1618..2604
                     /locus_tag="VKU03_01095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P1 family peptidase"
                     /protein_id="HLJ69880.1"
                     /translation="MNNLITDLDGVAVGAAEDERIASGVSIVVFETPAVASIAIHGGA
                     PAARDTALLEPEMTVPAVDALVLSGGSAFGLDAAGGAQARLLEIGRGYPVGPFRVPIV
                     PSASLFDLANGGDKNWGRRSPYWDLGYEAASKAGKAFALGTAGAGYGATTSDLKGGLG
                     SASAVSSRGFLVGALVAANAVGRATRGSCPHFWAAPYERGGEFGGLGEGRAAADALTM
                     RLKNEAPANTTIGVVVTDAALTKAQMKRVATMAQDGYALAIRPAHAPTDGDIVFAATT
                     GRAEQSPDLRDVTEIGMLAAECLARAIARGVYEAKSLDNSVAPRCWREKYGS"
     gene            complement(2824..>3270)
                     /locus_tag="VKU03_01100"
     CDS             complement(2824..>3270)
                     /locus_tag="VKU03_01100"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLJ69881.1"
                     /translation="PLGAKRGYHMHYGAKGNATLSRPVLDAEMGYVLTPMARGIRLTT
                     GAEFARRDDPPSSAHLDRLEPFARALFPLAERRDAAPWLGRRPCLPDMLPVIGPAPRR
                     ASLWLDFGHHHLGLTLGPISGRLLAEMMTGEKPSLDPRPFSAARFG"
BASE COUNT          514 a         1089 c         1154 g          513 t
ORIGIN      
        1 cgggcgcaaa agccttggac cagagcggtt tcggtcttgc cggcaagtcc ggcctccgcg
       61 atccacgcgg cgagcgcgac gatgtctttc tcgatcatga ggcgatcata atcctccccc
      121 gcggagcggg ggagggggga ccatgcgaag catggtggag ggggtacggc ggttggggat
      181 tatcctgcga gaccgcgccc cctccaccgc gctgacgcgc ggtccccctc ccccattccg
      241 cttcgctcca tgggggagga tgggcgcctc aaaacgtcgc gaaattacaa gtctccgccg
      301 tcgcgcatcg tcgtcggcag cgagcctgcg ggtcgccgcc gccatgcgag cctcccggtt
      361 gagcgcaacg ctgggatatt gtaacccgtc acacagtcgg cgacgatgaa caacgtcatt
      421 cccgcgaaag ccggaatctc gcgtatgcgc gaaggacacg cggcgctgga ttctcacttt
      481 cgcggaaatg gcagtgcaag ggtaaggagg acgtcatgcc cgtcaataag cagatcgtgc
      541 tcgattcgcg tccggccggc gccgccgagg cgggcaattt tcggctgatc gagagcgagg
      601 cgccgaatcc cgggccgggc caggttctcg tgcgccacct ctatctgtcg ctcgatcctt
      661 acatgcgcgg ccggatgagc gacgcgaaat cctacgccaa gccgcaggag ctcggcgcgg
      721 tgatggtcgg cggcacggtc ggcgaggtgg tcgcgtcgaa caatccgcga ttcaaggccg
      781 gcgacaaggt ggtcggcatg ggcggctggc agaattatgc gctgagcgac gggggcgatc
      841 ttcgcgtcgt cgacgcctcg cgcattccga tccaggccta tctcggcccg ctcggcatgc
      901 cgggcgtcac cgcctggtac ggtctcaaca agatcatcgc gccgaagaac ggcgagacgg
      961 tggtcgtcac cgccgcgacc ggcgcggtcg gctcggtcgt cggccaattg gcgaagcttg
     1021 ctggcgcgac cgcgatcggc gtcgccggcg ggccggagaa atgcgcctat gcgcgcgacg
     1081 agctcggcta ccatgtctgc atcgatcata aatcgccgag tttcgtcgcc gacttgaagg
     1141 ccgcgctgcc gaaaggcgtc gacggcctgt tcgaaaatgt cggcggcttg cctttcgaga
     1201 cggcggcgcg gcgcttcaac gatttcgccc gcgtcgcgat ttgcggcctg atcgcctcgt
     1261 acgacggcgc gccggcgccg gcgatcgatc tgcgcgtcat gctgaccaag cgcatcagga
     1321 tcgaaggctt catcgtcagc gatcacatgg cgctgtggcc gaccgcgctc gccgagctcg
     1381 ccggccatgt cgcggcgggc cgcatccgct atcgcgagac gatttcgcaa gggctggaaa
     1441 gcgcgccgga agcgttcctc ggcctgctca agggcaagaa tttcggcaag cagctcgtca
     1501 agctgtgacc gtcattgcga gcgaagcgaa gcaatcccgc ccctacgaat ggatcgcttc
     1561 gtcgcttcgc tcctcgcaat gacgacgtta agggctccgt acggggcgga aggacatatg
     1621 aacaatctca tcaccgatct ggacggcgtc gcggtcggcg cggccgagga cgagcgcatc
     1681 gcatcgggcg tcagcatcgt cgttttcgaa acgccggccg tcgcctcgat cgccattcac
     1741 ggcggcgcgc cggcggcgcg cgacaccgct ttgctcgagc cggaaatgac ggtgccggcg
     1801 gtcgatgccc tcgtcctttc gggcggctcg gctttcggcc ttgatgccgc gggcggcgcg
     1861 caggcgcgcc tgctggagat cggccgcggc tatccggtcg gtccgtttcg cgtgccgatc
     1921 gtaccgagcg ccagcctctt cgacctcgcc aatggcggcg acaagaattg gggccggcgc
     1981 tcgccttact gggatcttgg ctacgaagcc gcgtcgaagg ccggcaaggc cttcgcgctc
     2041 ggcacggccg gggccggcta cggcgcgacg accagcgatc tgaaaggcgg cctcggctcg
     2101 gccagcgccg tctcgtcgcg cggctttctc gtcggcgctc tggtcgcggc caacgcggtc
     2161 gggcgcgcca cgcgcggctc gtgcccgcat ttctgggccg cgccttacga gcgcggcggc
     2221 gaattcggcg ggctcggcga ggggcgcgcc gccgccgacg cgctgaccat gcggctgaag
     2281 aacgaagcgc cggccaacac gacgatcggc gtcgtcgtca ccgacgcggc gttgacgaag
     2341 gcgcagatga agcgcgtcgc gaccatggcg caggacggct atgcgctggc gatccgtcct
     2401 gcgcatgcgc cgaccgacgg cgacatcgtc ttcgcggcga cgaccggtcg cgccgagcaa
     2461 tcgccggacc tgcgcgacgt gaccgagatc ggcatgctcg ccgccgaatg cctggcgcgg
     2521 gcgatcgcgc gcggcgtcta tgaggcgaag tcgctggaca attccgtcgc gccgcgctgc
     2581 tggcgggaaa agtacggatc gtaacgtttc gctcggaact gcggggcgag cgcgagcagg
     2641 cgctacgcgt tcgccctcac cccaaccctc tcccgcaagc gggagagggg gcgcgtcgcg
     2701 gccgtcatcg accgccgcgg tgaagccgtg atcgccgatc gcggctacaa ttccttctcc
     2761 cgcttgcggg agagggtggc gcgaagcgcc ggatgagggt gcgccgcgcc ccgcgaaaga
     2821 gcgtcacccg aagcgggcgg cgctgaacgg ccgcggatcg agcgacggct tttcgccggt
     2881 catcatttcg gcgagcagcc tgccgctgat cggcccgagc gtcaggccga gatggtgatg
     2941 gccgaaatcg agccaaaggc tcgcgcgcct cggggccgga ccgatgaccg gcagcatgtc
     3001 gggcaggcat ggccggcggc cgagccaggg cgccgcgtcg cggcgctcgg ccagcgggaa
     3061 caaggcgcgg gcgaagggct cgagccggtc gagatgggcg ctggacggtg gatcgtcgcg
     3121 ccgcgcgaac tcggcgccgg tcgtcagcct tatgccgcgc gccatcggcg tcagcacgta
     3181 gcccatttcc gcgtcgagca ccggccggct cagcgtcgca ttgcccttgg ccccgtaatg
     3241 catgtgatag ccgcgcttgg cgccgagcgg
//
LOCUS       DATGJW010000037         3201 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1354330, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000037 DATGJW010000000
VERSION     DATGJW010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3201)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3201)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3201
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1354330"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..797)
                     /locus_tag="VKU03_01105"
     CDS             complement(219..797)
                     /locus_tag="VKU03_01105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HLJ69882.1"
                     /translation="MKERPASTNASDLRRLTDEEIELWRAVAKSVAPRKGVILPASRK
                     RFAPAATPIGTEKPAAAPKARAPQSLALAPLEKQLRRRLARGGAGPDDAIDLHGMTEA
                     EAHRALRGFLSYAQARGARLVLIITGKGEGKGRASPLWAEEPGVLRRLAPHWLRAPDL
                     RAVVLGFEEAGRSHGGAGALYVRLRKPREGTR"
     gene            complement(794..1888)
                     /locus_tag="VKU03_01110"
     CDS             complement(794..1888)
                     /locus_tag="VKU03_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HLJ69883.1"
                     /translation="MRRALAPARFDDLAGFAQDDHLDAFRAWRRSAQALIAGVRPTRP
                     GAAIPPAFRDVARIALARDVTDCKAARDFFVAHFRPYRVRPDRGQAGFVTGYYEPLLR
                     GALSPAPGFSAPVLGRPDDLVSFAPGETPPDLDPQLSGGRRGVGGRFEPYPDRAAIEA
                     AAESGAMRPILWLEDHVELFLAQVQGSARVALPDGRVVRLAYDGRNGQPYTSIGRALI
                     EEGAIKESEMSLAALKAWLRRDRQAGRALMRRNRSYVFFRLEENFDPSAGPTGGAGIA
                     LTKLRSIAIDRRLWPYHAPFWIEAMLPWRGPSPSPFRRLMIAQDTGSAILGAARADIF
                     FGDGEEAGQRAGDIRREAALTVLLPVGAEL"
     gene            complement(1944..2669)
                     /locus_tag="VKU03_01115"
     CDS             complement(1944..2669)
                     /locus_tag="VKU03_01115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HLJ69884.1"
                     /translation="MPPVDATTIVFAIIAIFVVWKLYSVLGTRTGAEPPPFDSNLNRD
                     SASAGPGAPRGPGQIIRLPGAAAPSAPAAPPPLPDPQRWKDFIEPGSKAASGLDAIAA
                     ADRSFDAAGFLSGAKIAYESIVLAFARGDRNALAGLLATDVLDNFSRAINERLARGET
                     METNIVSIDSAKVEDARLVGGAAQIAVRFAAKLISSTRDKAGTVIEGSADQVADHLDM
                     WTFARDISSRNPNWRLIATESGH"
     gene            complement(3017..>3201)
                     /locus_tag="VKU03_01120"
     CDS             complement(3017..>3201)
                     /locus_tag="VKU03_01120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69885.1"
                     /translation="GGFLYDWIMKVASADDIIAALRAHEGELRQAGIRRLSLFGAAAR
                     GAGEASDIDLAAELDP"
BASE COUNT          537 a         1104 c         1080 g          480 t
ORIGIN      
        1 cccatatgcg catagagcgc ctcggcccgc gcctcgtcga tcagagtcat gccgcctttt
       61 tgaatctttt cggcgcgcag gaaatcgtcc tcggcggggg caagaatatc gaccagcgaa
      121 ttgccgaggc cgagcacttc gatccgttcg cgcgtcatgc gtggaagggt ctccttttta
      181 aggtctcgtc gcatttgagg cgccgagggt caatggcgtc agcgcgtccc ctcgcgcggc
      241 ttcctcaggc ggacgtacag cgctcccgcc ccgccatggc tgcggccggc ctcttcgaag
      301 ccgagcacga cggcgcgcag gtcgggggcg cgcagccaat gcggcgccag ccgccgcagc
      361 acgcccggct cctccgccca gagcggcgag gcgcgccctt tgccttcgcc cttgccggtg
      421 atgatcagga ccagacgcgc gccgcgcgct tgcgcatagg acaggaagcc gcgcagcgcg
      481 cgatgggcct cagcctcggt catgccgtgc agatcgatcg cgtcgtccgg cccggcgccg
      541 ccgcgggcga ggcggcgcct tagctgtttt tccagcggcg ccaaggccaa gctttgcggc
      601 gcccgggcct ttggcgccgc cgccggcttt tccgtcccga tcggcgtcgc cgccggcgcg
      661 aaacgcttgc gcgaggccgg caggatgacg cccttgcgcg gcgcgacgct tttggcgacg
      721 gcgcgccaga gctcgatctc ctcgtcggtc aaacgccgca aatcggatgc gttcgtactg
      781 gccgggcgct ccttcacagc tccgctccga ccggaagaag gacggtcaac gcggcctcgc
      841 gccgtatgtc gccggcgcgc tggccggcct cctccccgtc gccgaagaaa atgtcggcgc
      901 gcgcggcgcc gagaatggcc gagccggtat cctgggcgat catcaagcgg cgaaacggag
      961 acggcgaggg accgcgccac ggcagcatcg cttcgatcca gaacggcgcg tgataaggcc
     1021 aaagtcgccg atcgatggcg atcgaacgca gtttcgtcag cgcgatcccg gcgccgccgg
     1081 tcggcccggc gctcggatcg aaattctcct caagcctgaa aaaaacgtat gagcgattgc
     1141 gccgcatgag cgcgcgcccg gcttggcgat cgcgccgcag ccaggctttc aacgcggcga
     1201 gcgacatctc gctctccttg atcgcgccct cctcgatcaa ggcgcggccg atcgacgtat
     1261 aaggctggcc gttgcgtccg tcataagcaa ggcgcacgac gcggccgtcc ggcagcgcga
     1321 cgcgggccga gccttgcacc tgcgccagga acagttcgac atgatcttcg agccacagga
     1381 tcggccgcat cgcgccgctt tccgcggcgg cttcgatcgc cgcgcggtcg ggatagggct
     1441 cgaagcggcc gccgacgccg cgccggccgc cggaaagctg cggatcgaga tcgggcggcg
     1501 tctcgcccgg ggcgaagctg acgagatcgt ccggccggcc gaggaccggc gccgaaaatc
     1561 ccggagcagg cgaaagcgcg ccgcgcagca gcggctcgta atagccggtg acgaagccgg
     1621 cctggccacg atcggggcgg acgcgatagg ggcggaaatg cgcgacgaag aagtcgcgcg
     1681 ccgctttgca gtcggtcaca tcgcgcgcga gcgcgatccg cgcgacgtcg cgaaaagccg
     1741 gcgggatcgc cgctcccggc cgcgtcggcc gaacgccggc gataagcgcc tgcgccgagc
     1801 ggcgccacgc gcgaaaagcg tcgagatgat cgtcttgcgc aaagccggca agatcgtcga
     1861 aacgcgccgg cgccaacgct cgtcgcaaag ccgcctcttc gccgcgcgcg cctgcgttcg
     1921 cccggcccat ccgcgcgtcc cggctaatgg ccgctttcgg tcgcgatcag ccgccagttc
     1981 ggattgcgcg aggatatgtc gcgggcgaag gtccacatgt cgagatgatc ggcgacctgg
     2041 tcggccgaac cttcgatcac cgtgccggct ttatcgcggg tggacgagat cagcttcgcc
     2101 gcgaagcgca cggcgatctg cgccgcgccg ccgacaagcc gcgcatcctc cactttcgcc
     2161 gagtcgatcg agacgatatt cgtctccatc gtctcgcccc gggcgaggcg ctcattgatc
     2221 gccctggaga aattgtcgag cacgtcggtc gcgagcaggc ccgccagcgc attgcgatcg
     2281 ccgcgcgcaa aggcgaggac gatggattca taggcgattt tcgcgccgct gaggaatccc
     2341 gccgcatcga agctgcgatc ggccgcggcg atcgcgtcga gcccggaagc ggctttcgag
     2401 cccggctcga taaaatcctt ccagcgttgc ggatcgggaa gcggcggcgg cgcggccggc
     2461 gcgctcgggg cggctgcgcc gggtaagcgg atgatttggc cgggcccgcg cggcgcgccg
     2521 ggaccggcgc tcgccgaatc gcggttgaga ttggaatcga aaggcggcgg ctcggcgccc
     2581 gtcctcgtgc cgagcacgga ataaagcttc cacaccacga aaatggcgat gatagcaaag
     2641 acgatcgtcg tagcatccac aggcggcatc agcacaactc ttcagctcgc gcccgcgccc
     2701 gccctgcaat gaggggaggc gccaaatcac gtcgtgtctt ggacagatag agcttgtcgc
     2761 gaagcttcgc cagccccgcc gcgtagcgcc cagggttact tcggattaat gcgccgaatc
     2821 cgctccaagg ccagtcgcga gtcggccgct aggccgggca aatcctccgg aatggagttc
     2881 cagatcacct ctaggccgac ccggtcatag gcatggcgca ggcgattgcc tgccgcggcg
     2941 aatgcgccca aggctggttg ggcgtgagct ggtcgccaag acggaacgct gcctcgcatc
     3001 cgctcgaagc agcgccctac gggtcgagtt cggcggcaag atcgatatcg cttgcctcgc
     3061 ccgcgccacg cgccgcggcg ccgaacaagg acaaccggcg aatgcccgct tgccgcagtt
     3121 cgccttcgtg cgctcgaaga gccgcgataa tgtcgtcagc cgaagcgact ttcataatcc
     3181 aatcatagag gaagccgccg c
//
LOCUS       DATGJW010000038         5517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_213633, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000038 DATGJW010000000
VERSION     DATGJW010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5517
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_213633"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            464..>5517
                     /locus_tag="VKU03_01125"
     CDS             464..>5517
                     /locus_tag="VKU03_01125"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HLJ69886.1"
                     /translation="MISKTAQKPLPSFVFAAGGAVLVAAGLWASLALQAWFAAALLGA
                     AGLAGWRYAATRSPGSPVEIALDAAAFAIFAIMRNNALGFWQLPGPWKDVPFFNPSGA
                     EIAYAVYLGGFLAALTLGGRGLRAVEALALMATPFLFNLLMTVAADWHMQELGALLTP
                     GLPLSFQAQVFVGRAAILFVVAEAMLAAFATMRMGRPALDPKLHGLLIVVAALAAATP
                     PLANFAQLAGFSPALAILAGALFAALAQAGLWSIVYALTGVALDWIGGKPPSTLSVYG
                     HWRAGLVKGAIYGGLFMFGILAVAAPLRVPAFAAFAQTNALLLSPLVGALAFPLAATI
                     VGSADGTPPFFGRLAAAYRNPRAYARGAVAGLGAALALTSNLRDADGSTRFLSLFLVG
                     ALAYAGVDLLFDLASIFRGERRVLQTWRLYALGAGLGGLVAGALGWYFDAPQIKVVVD
                     KFWAYADINYTLTGRHLSDFTTFPIFNKYGAIDLGEIVGGVRLFYTESLAGVINWSIA
                     APLFGVNFVLLNSLFDRSLRPIKQLVSEAGIEGLVAQTVRVMRWGLWMAPIINSFLRQ
                     QPEPSWFNQDGAIRTIVSIGADIGLPAIDFHNFSLTVFLGLLAYDWLRVLIWFDHMGL
                     RVATLVNLTFIGGDRADEAAARYLGHAARTRVVPDGIRRFSTWAPLLIPFYIPRGADW
                     DHAWTGAEALRHSGAALPGPVNIMLIAYCCAGCAFAAAAVIAGSRMREKTGAAAPALA
                     GAPPALDGTPLEVSLTNGAVGLDMMRDGRGAAFVTGEERGGFPIDLTRRPLDPLQARG
                     VFFYIREGEGEPWSIGYEPARRAGLYRFEETGRNGVVIANEIDGLEARMEAGPDALGA
                     VLSWRIGLVDRSGKKRRLRLASFSEIAAHETGAYMRDLDFAGMHVETFFMRDLNTIMA
                     RNRLLRSKRAGRSEMAFFAARPLSEGVRLIGYEDSRARFLGEGSLAKPQGLDEKRPRA
                     LDDQGKLWTFDPAASLSFDIEIEANGAAELEFIIGRADNERAAADLIARRLALPPIGK
                     EELAARVYHTRMVEPTPQPASRWPFAFSADGIELRLTNRTPRPWAHVMANELGAGAVV
                     SNDGEVYSFFGNAQQNGLTPYRFDSVTIPQPGQVVYLRNLDTDETDSIGFTPFQCEDA
                     TYDVTYEPGVATFRKKRRGLSVAYSVFTPPDFRGDMRLLSLSNGGEKPLSLRIVPFFD
                     IALAETPNASKDLLSVEQVGSALLFENPRNDFQRGTAFVATSLEDPAIETIRKRFFGG
                     RGRNIVSPVLVETGMADASQHDDGRRVAAFAREIQLRPGEEMKIAIVFGQEKDRDAAL
                     AAAAKADVDEVEAALAHTRRVWRERLSHVHIKTNRPDFDRLVNTWLPYQTMASRLFGR
                     VGPNQRGGAFGFRDQLQDVLPLVFIEPRLTRAQIVLHAGQQFLEGDVLKWWHRAPDGR
                     TGLGQRTKASDPHLWLPYVATRYVEATGDSSVLDEKTAYLEGPAVPEHEDTWVIVPRA
                     SREIGSVYEHCQRAIDYTLRHMGANGLPLLGAGDWNDGIDGLGREGRGVSVWMGFFFY
                     DVLTRFAPLAAARGDKEYAKTCDEARAGLRKALEIGWQGTHYALDFADNGQPLAMPNA
                     MTTGWPAYSGAVAFERGVAAIEGGLKGIERPDRILLTETPFFEHSSPYPGRIADYPPG
                     VRENGGQYSHGASWIV"
BASE COUNT          795 a         1898 c         1799 g         1025 t
ORIGIN      
        1 tgggcatggg ctgactggga gctggtcggt gaaagccggc ggcgctttcc tatatccccc
       61 gcttcgacaa gggaagggcc gccacgagag gctcgccgct cacgacgtcc ggcgcgccgc
      121 cttgtcggga cgcggccgta ccagagcacg ccaacgaagc cggcggctaa ggaatcgccc
      181 tgatccagtc gccatcgagg cggcgcatct caaatttgac gccaaaatcc cctgttgcag
      241 atgggattcg acctcggcct ctcggcggaa gaccgtcccg cgcgcaagcg cttgatgttc
      301 cttcgccaat ccgatcccct cggccgcgat cggcggccga tgaggccgtt tttcgatctg
      361 accacacgaa tgccttatcc ccaccttagc tggccgcgcg gccgctttcg cttgcgcgcc
      421 gtttatcatt ggcccaagcc ttggctcggg agacgcgttg catatgattt cgaagacggc
      481 gcaaaagccc cttccttcct tcgtgttcgc cgcggggggc gccgtcctcg tcgcggctgg
      541 gctctgggct tcgctcgcgc tgcaggcttg gttcgcagcg gcgctgcttg gcgccgccgg
      601 tctggccggg tggcgctatg cggcgacgcg cagccccggc tcgccggtcg agatcgcgct
      661 cgacgccgcc gctttcgcga ttttcgcgat catgcggaat aacgcgctcg gcttctggca
      721 attgccgggg ccttggaagg acgtcccttt cttcaacccg tccggcgcgg agatcgccta
      781 cgcggtctat ctcggcggct tcctggccgc cctgacgctc ggcgggcgcg gcttgcgcgc
      841 ggtcgaagcc ttggcgctga tggcgacgcc gttccttttc aatctgctga tgaccgtcgc
      901 cgccgattgg cacatgcagg agctcggcgc gttgctgacg cccggcctcc cgctgtcctt
      961 tcaggcccag gtctttgtcg gccgggcggc gattcttttc gtcgtcgcgg aagcgatgct
     1021 cgccgcgttc gcgacgatgc gcatgggacg gccggcgctc gatcccaagc tgcacggtct
     1081 gctgatcgtc gtcgccgcgc tcgccgccgc gacgccgccg ctcgccaatt tcgcccagct
     1141 cgccggcttc tcgccggctc tcgccattct cgccggggcc ctcttcgccg cgctggcgca
     1201 ggcgggcctg tggagcatcg tctatgcgct gaccggcgtc gcgctcgatt ggatcggcgg
     1261 caagccgccg agcacgctct ccgtgtacgg acattggcgc gccggcctgg tcaagggcgc
     1321 gatctatggc ggcctgttca tgttcggcat tctcgccgtc gccgcgccgc tgcgcgttcc
     1381 cgccttcgcc gccttcgccc agaccaacgc gcttcttcta tctcccctgg tcggggcgct
     1441 cgcctttccg ctcgccgcga cgatcgtcgg cagcgccgac ggaacgccgc cctttttcgg
     1501 ccgcctcgcc gccgcctacc gcaatccgcg cgcctatgcg cgcggcgcgg tcgccgggct
     1561 cggcgcggct ttggcgctga cctccaacct tcgcgacgcc gacggctcga cgcgctttct
     1621 ttcgctcttc cttgtcggcg cgctcgccta tgccggcgtc gatcttctgt tcgatctcgc
     1681 cagcattttc agaggcgagc gccgcgttct tcagacctgg cggctctatg cgctcggcgc
     1741 cgggctcggc ggcctcgtcg ccggcgcgct cggctggtat ttcgacgcgc cgcagatcaa
     1801 ggtcgtggtc gataaattct gggcctatgc cgacatcaac tatactttga ccggccgcca
     1861 tctcagcgac ttcaccacct ttccgatctt caacaaatac ggcgcgatcg acctcggcga
     1921 gatcgtcggc ggcgtgcgct tgttctacac cgagtcgctt gccggcgtga tcaactggtc
     1981 gatcgccgcg ccgcttttcg gcgtcaattt cgtactgttg aacagcctgt tcgatcgcag
     2041 cctgcggccg atcaagcagc ttgtcagcga agcgggaatc gaaggcctcg tcgcccagac
     2101 cgtccgggtg atgcgctggg gcctctggat ggcgccgatc atcaattcgt tcctgcgcca
     2161 gcagccggag ccgagctggt tcaatcagga cggcgcgatc cgcacgatcg tctccatcgg
     2221 cgccgatatc ggcttgccag cgatcgactt ccacaatttc agcctcaccg tgttcctcgg
     2281 actgctcgcc tatgactggc tgcgggtgct gatctggttc gaccatatgg gcttgcgcgt
     2341 cgcgacgctg gtcaatctga cctttatcgg cggcgaccgc gccgacgagg cggcggcgcg
     2401 ctatcttggc catgcggcgc gcacgcgcgt cgtgccggac gggatccgcc gtttctcgac
     2461 ctgggcgccc ctgctgatcc cgttctacat tccgcgcggc gcggattggg accatgcttg
     2521 gaccggcgcc gaggccttgc gccattcggg cgcggcgctg cccgggccgg tcaacatcat
     2581 gctgatcgcc tattgttgcg ccggctgcgc cttcgccgcc gccgccgtga tcgccggctc
     2641 gcgcatgcgc gagaagaccg gcgccgcggc gcctgctttg gccggcgcgc cgccggcgct
     2701 tgacgggacg ccgctggagg tgtcgctgac caacggcgcg gtcggcctcg acatgatgcg
     2761 cgacgggcgc ggcgcggctt tcgtgaccgg cgaggagcgc ggcggctttc cgatcgatct
     2821 gacccggcgg ccgctcgatc cgctgcaggc gcgcggcgtg ttcttctaca tccgcgaagg
     2881 cgagggcgag ccgtggtcga tcggctacga gccggcccgt cgggccgggc tctatcgctt
     2941 cgaggagacc ggccgcaacg gcgtcgtcat cgccaacgag atcgacgggc ttgaggcgcg
     3001 gatggaggcg ggaccggacg cgctcggcgc ggttctgtcc tggcgcatcg gcctggtcga
     3061 caggtcgggc aaaaagcggc ggctccgcct ggccagcttc agcgaaatcg cggcccacga
     3121 gaccggcgcc tatatgcgcg atctcgattt cgccggcatg catgtcgaaa ccttcttcat
     3181 gcgcgatttg aacacgatca tggcgcgcaa ccggctgttg cgctcgaagc gcgccggccg
     3241 cagcgagatg gctttcttcg ccgcgcggcc gctttccgag ggcgtccggc tgatcggcta
     3301 tgaggattcg cgcgcgcgct ttctcggcga aggatcgctc gccaagccgc aggggctcga
     3361 cgaaaagcgg ccgcgagcgc ttgacgatca aggcaagctc tggactttcg atcccgccgc
     3421 gagcctgtcc ttcgacatcg agatcgaagc gaacggcgcg gcggaactcg aattcatcat
     3481 cggccgcgcc gacaatgagc gcgccgccgc cgacctgatc gcgcgccgcc tcgctttgcc
     3541 gccgatcggc aaagaggagc tcgcggcccg cgtctatcat acgcgcatgg tcgagccgac
     3601 gccgcagccg gcgtcgcgct ggcctttcgc cttttccgcc gacgggatcg agttgcggct
     3661 gaccaaccga acgccgcggc cttgggcgca tgtcatggcc aatgagcttg gcgccggcgc
     3721 ggtcgtctcc aatgacggcg aggtctattc tttcttcggc aatgcgcagc agaacgggct
     3781 gacgccgtac cgtttcgaca gcgtcacgat cccgcaaccg gggcaggtcg tctatctgcg
     3841 caatctcgac acggacgaga ccgactcgat cggcttcacg cccttccaat gcgaggacgc
     3901 gacctacgac gtgacgtacg agccgggcgt cgcgaccttc cgcaaaaagc ggcgcggcct
     3961 gtcggtcgcc tattcggttt tcacgccgcc ggatttccgc ggcgatatgc ggctgctttc
     4021 cctctccaat ggcggcgaaa agccgctgag cctgagaatc gttccgttct tcgacatcgc
     4081 gctcgccgag acgcccaacg ccagcaagga tctgctctcg gtcgagcagg tcggcagcgc
     4141 gcttctcttc gagaatccgc gcaacgattt ccagcgcgga acggcttttg tcgcgaccag
     4201 cctggaagac ccggccatcg agacgatccg caagcgcttc tttggcgggc gagggcgcaa
     4261 catcgtttcg ccggttctgg tcgagaccgg catggccgac gcttcgcagc acgatgacgg
     4321 ccggcgcgtc gccgctttcg cgcgggaaat tcagcttcgc cccggcgaag aaatgaaaat
     4381 cgcgatcgtc ttcggccagg agaaagaccg cgacgccgcg ctcgccgctg cggcgaaagc
     4441 ggatgtcgac gaggtcgaag cggcgctcgc gcatacccgc cgcgtttggc gcgagcggct
     4501 gagccacgtg catatcaaga ccaaccgccc cgacttcgat cggctggtca atacctggct
     4561 gccttatcag acgatggcct cgcgcctgtt cggccgggtc gggccaaacc agcgcggcgg
     4621 cgccttcggc ttccgtgacc agcttcagga tgtcctgccg ctggtcttta tcgagccgag
     4681 actgacgcgg gcgcagattg tgctgcatgc cggccagcaa ttcctcgaag gcgatgtgct
     4741 caaatggtgg catcgcgcgc cggacggccg caccggtcta ggccagcgca ccaaggcgag
     4801 cgatccccat ctgtggctgc cctatgtggc gacgcgctat gtcgaggcga cgggcgacag
     4861 cagcgtgctg gacgagaaga cggcctatct cgaaggcccc gccgttcccg agcatgagga
     4921 tacatgggtg atcgtgccgc gcgcctcgcg cgaaatcggc tcggtctacg agcattgcca
     4981 gcgcgcgatc gattacacgc tgcggcatat gggcgccaac ggcttgccct tgctcggcgc
     5041 cggcgattgg aacgacggga tagacgggct cggccgcgag ggccgcggcg tcagcgtgtg
     5101 gatgggcttc ttcttctacg acgtgctcac gcgtttcgcg ccgctcgctg cggcgcgcgg
     5161 cgacaaagaa tatgcgaaga catgcgacga agcccgcgcg ggtctccgca aagccttgga
     5221 gatcggctgg caggggacgc attacgcgct tgactttgcg gataatggcc agccgctcgc
     5281 catgcccaac gcgatgacga caggctggcc ggcttattcc ggcgccgtcg ccttcgagcg
     5341 cggcgtcgcc gcgatcgaag gcggcctgaa agggatcgag cggccggatc gcatcctgct
     5401 gaccgagacg ccgttcttcg agcattcttc gccctatccg ggccgcatcg ccgactatcc
     5461 gccgggcgtg cgcgagaatg gcggccaata tagccacggc gcgtcctgga tcgtcga
//
LOCUS       DATGJW010000039         9371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1251109, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000039 DATGJW010000000
VERSION     DATGJW010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1251109"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1378
                     /locus_tag="VKU03_01130"
     CDS             <1..1378
                     /locus_tag="VKU03_01130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493626.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heme biosynthesis HemY N-terminal
                     domain-containing protein"
                     /protein_id="HLJ69887.1"
                     /translation="WGIIRFIFRIPSLMTLARRARRREKGMAALSRGMIAVGSGDARA
                     ARRHAAEAHRLIGDEPLTHLLRAQAAQLAGDRAAAEAAFRDMLGKASTHALGLRGLHL
                     EARRRGDHAAALQYAAEANKHAPLPWAGQAVLDHRAAQGDWGGALATVESNAAAHLID
                     KPTAHRWRAVLKTALAQERAQREPQAALALAQEAVRLAPDLAPAAALAGRLLAAQGDY
                     RRASKILETSFAKTPHPDLAATYLRMRPGDSASDRLLRARALARVAPNDPESRLTVAR
                     AALEARDFKAARDAIGPLIEADAPAGRPTRRVCLLMADLEETEGGAPGAVREWLARAS
                     RAPHDPAWIAEGIISDVWAPASPSGKLDAFVWRAPDERLSAPVEPPPAREEPTMIEHV
                     AETPEAPEPSVRKEEPPSMEPPLSTAIEKKPGPAAPRSVIDLPPPNPPDDPGADGAPE
                     KPRFRLFS"
     gene            complement(1406..2527)
                     /locus_tag="VKU03_01135"
     CDS             complement(1406..2527)
                     /locus_tag="VKU03_01135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLJ69888.1"
                     /translation="MDEKKTSAGCIGGPLAGVRMVEFASIGPGPFAAMLLADMGAEII
                     AIDRPEPSPFRPGAILSRGRPAVIADLKDPAQRDEALSLIASADALIEGFRPGVMERL
                     GLGPDVALKRNPKLVYGRMTGWGQTGPLAGSAGHDINYIALAGALAAIGTPEQPIAPL
                     NLLGDFGGGSLYLVAGLLAAIIAARRTGEGQVVDCAICDGAASLMAMCAEMSAAGRWR
                     KARGANLLDGGAPFYRTYVCADGEHIAIGALEPRFYARLCALIGLDETIDRDDPANWP
                     ALREKMAALFKTRTREEWRARLEGTDACFAPVLTLAEAARHPHLEARETFVAIDGVTQ
                     PAPAPRFSRTPAAIRAIEERPRTLAEAAAAWGGDGKAAS"
     gene            2928..4208
                     /locus_tag="VKU03_01140"
     CDS             2928..4208
                     /locus_tag="VKU03_01140"
                     /inference="COORDINATES: protein motif:HMM:NF024189.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69889.1"
                     /translation="MKPNSLSCAARASFAAALLGAVCLVGSPAFAQEEACAPPTVVDQ
                     PPPPLPDYDQPPIPAPGYIWSPGYWAWDSDESDYYWVPGTWTEPPRPGLLWTPGYWAW
                     VAGAYVFHAGYWGPRVGYYGGIHYGYGYTGEGYEGGRWEHGTFFYNRTVNNIGNVTIP
                     NVYEKKIVINNNTTTNISYNGGKGGVEAKPTPEDKTYARQAHFAPTPAQRQHVTTASK
                     DPALFEKTNHGAPAVAATPRPADFKGPGVVPAHAAATQPGAAGKKPEEQKPAPGKPEL
                     EQKKPAAGAVQPGVEEKGKTPLTEEKKPAEGNKPALEQKKPLTEEKKQMEGNKPAVEE
                     KKPAIQEKKPAIEEKKPVIEEKRPTIEEKRPVEEKRPQPTRPETQSRPEPMRPQPQAR
                     PEPKMQMEKPAMRPQPPAGAHPPGKPEEKEKKQP"
     gene            4261..4616
                     /gene="ssrA"
                     /locus_tag="VKU03_01145"
     tmRNA           4261..4616
                     /gene="ssrA"
                     /locus_tag="VKU03_01145"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF01849"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="alphaproteobacterial-type; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /db_xref="RFAM:RF01849"
     gene            4683..5246
                     /locus_tag="VKU03_01150"
     CDS             4683..5246
                     /locus_tag="VKU03_01150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ClpXP protease specificity-enhancing factor
                     SspB"
                     /protein_id="HLJ69890.1"
                     /translation="MPTDLIRYDLLVQDALRAVVRRVLSDAARNGLPGEHHFNISFKT
                     QAPGVALPAAIRQKYPDEMTIILQHEFWDLAVEADGFEVSLNFSRRPERLKIPFDAIV
                     GFSDPSVPFGFKLEPREQAAASAPALEKQEPPPAPLAKPQPAPLPAPAAKPAASGASR
                     PAAGETRKTPEKDDATKVVSIDAFRKK"
     gene            5254..5448
                     /locus_tag="VKU03_01155"
     CDS             5254..5448
                     /locus_tag="VKU03_01155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4169 family protein"
                     /protein_id="HLJ69891.1"
                     /translation="MGEIVNLRRTRKRQAKAAAEDEAAANRLAFGRSKEERRRLKAER
                     EAQAKRLDGHRLDKPVGNGE"
     gene            5438..5671
                     /locus_tag="VKU03_01160"
     CDS             5438..5671
                     /locus_tag="VKU03_01160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HLJ69892.1"
                     /translation="MASDEARGAAIVKRSIAIAGHRTSISLEDAFWRELKALARRRGR
                     SVSALVAAIDAERKSANLSSAIRVFVLEALAER"
     gene            complement(5798..>9371)
                     /locus_tag="VKU03_01165"
     CDS             complement(5798..>9371)
                     /locus_tag="VKU03_01165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69893.1"
                     /translation="DGGADLSCEEAKLELSLAPLVRGEIRFTDAVFERPRIEIKRNAG
                     GAIASPLLNVKVAPESVGLDTIALHDGSIVIDGAAGAPPIAIGGVFLDAQADSLRGPF
                     RGRGRAAGPDGRAVAFHFATGVMQKGALPIKVAADLGPAAPRAEFDGALALGASDFGY
                     AGGAVFSGAFARPGAAPTSWRIAGDLSANFETAKLKKLEARLGEEPRGLTLQGSAEAH
                     FGASPRLAVTLGGKELNLDALLRGKGEDSAPPERAYRAIAAFLSNAGLDRGPPLPVTV
                     KIDAPGIILGGDALNDAELDASAAPGAPVTLGVQALAPGQTRLAASGEVELGPASHFK
                     GALDIRVGDLDRLRDWISQGDDALHGRLAAIGAALPYSAASLAGDVDLSETSFVARNM
                     TLGLARSTLKGDLAWTRAVGADRDRLFLDLKTDSLDLDALPNFAAGGDFLRNVDLNLT
                     LDADALHLARFGEGAVDSGSLNLRLSKAGDDVTLDRLAIADLGGATIEADGALKPSER
                     WLNVDVDAQRLRDFALVLRRIAPSAAAQKLVERAGALSPAKLTFEARATGAAKDSVFA
                     PDTLAIRGVMGATKIAAKIDRPADSGGKLVATASLDAPEVSVLLRQFGLQAPPLTGLG
                     RGAVDLRAEGDWSRGFAGEATASLAGADLTWRGRALDPTATASGALTLKSANLLPLLA
                     AFGVVSREASGRAGADLSADLTWRGGEARLSRVKGVAAGSAISGALTYQAAPPQAGAA
                     AMPGAPPADPPPQIQGALTLDRLPLGALAALALGPAQPAKAGQPWSDLKFSPVVANPP
                     STDVALTIGSLDLMNGLAAHDANIRLKIGPTATALDDVSMRVAGGAVSGHATLRRDGA
                     TAAIAGRIALDSISLDRAGLNAKLAGVIDFAATGESQAALVAGLAGSGHIDLAGAELP
                     RLDPGALARVVAAAQASGFDIQNADINHMLAVEMDRKPMKIADQSAPAALSAGVLRVG
                     PLTAADQTGDAKVQASLDLRSFDLDIRAAISEKQAPKQFWSGPPPAMTVALKGRVGAL
                     ARDIDAASLANGLEAQAIARETERIAEFEADVRERAFFNRRLKAAHFMRQRELELQAY
                     AADQAQLKSEAERRRVEDDLLKASEDAREAEEARKTDEARQAEEAKKADDARKAEEEA
                     RKLLAAPPLPPPVPDAPPNSLASPTALQPPAKPPPRRLDPTGIY"
BASE COUNT         1583 a         3142 c         3247 g         1399 t
ORIGIN      
        1 gtgggggatc atccgtttca tctttcgcat tccctcgctg atgacgctgg cgcgacgggc
       61 gcgccgacgc gagaaaggca tggccgcgtt gtcgcgcggc atgatcgcgg tcggctccgg
      121 cgatgcgcgc gctgcccggc gccacgcggc cgaagcccat cgcctgatcg gcgacgagcc
      181 gctgacgcat ctgttgcgcg cgcaagccgc gcagctcgcc ggcgaccgcg ccgccgccga
      241 ggcggcgttt cgcgacatgc tcggcaaggc gtcgacccac gctttgggcc tgcgcggact
      301 gcatctcgaa gcgcgccggc gcggcgatca cgccgcggcg cttcaatacg ccgccgaagc
      361 gaacaagcac gcgccgctgc cctgggccgg ccaagcggtg ctcgatcacc gcgcggcgca
      421 aggcgattgg ggcggcgcac tggcgacggt cgagagcaac gccgccgccc atctcatcga
      481 caagccgacc gctcatcgct ggcgcgccgt cctcaaaacc gccttggcgc aagagcgcgc
      541 ccagcgcgag ccgcaagccg cgctcgcctt ggcgcaggag gccgtgcggc ttgcgcccga
      601 tctcgcgccg gctgcggcgc ttgccggcag gctccttgcc gcgcagggcg actatcggcg
      661 cgcctcgaaa atcctggaga cgagcttcgc caagacgccg catcccgatc tcgctgcgac
      721 gtacctccgc atgaggccgg gcgattcggc gagtgatcgc ctgttgcggg cgcgggcgct
      781 tgcccgcgtc gccccgaacg atccggaaag ccggctgacc gttgcgcgcg ccgcgctcga
      841 agcgcgcgat ttcaaggccg cgcgtgacgc catcgggcca ctgatcgaag cggacgcccc
      901 ggccggccgg ccgacgcggc gcgtctgtct cttgatggcc gatctcgaag agacggaggg
      961 cggcgcgccg ggcgccgtgc gcgaatggct ggcccgcgct tcgcgcgccc cgcacgatcc
     1021 ggcttggatc gcggagggga tcatcagcga cgtttgggcg ccggcttcgc cgagcggcaa
     1081 gctcgacgcg ttcgtttggc gcgcgcccga cgagcgcctg agcgcgccgg tcgagccgcc
     1141 gccggcgcgg gaggagccga cgatgatcga gcatgtggct gagacgccgg aggcgcctga
     1201 gccgagcgtt cggaaggaag agccgccatc catggagccg ccgttgagta cggcgatcga
     1261 aaagaagcct gggcctgccg ctccacgctc tgtgatcgac ttgccgccgc ccaatccgcc
     1321 ggatgatccc ggcgctgacg gcgcgcctga gaaaccgcgt ttcaggttat tctcgtgacg
     1381 cctggcgcca ttgcgccgct cgccgtcagc tcgccgcctt cccgtctccg ccccaggccg
     1441 ccgccgcctc cgcgagcgtc ctcggccttt cctcaatcgc tcgaatcgct gcaggagtcc
     1501 gcgaaaaacg cggggccggc gcgggttgcg tcacgccgtc gatcgcgacg aacgtttcgc
     1561 gcgcttcaag atgcgggtgg cgcgccgctt cggcgagtgt gaggacgggc gcaaagcagg
     1621 cgtcggttcc ttccaggcgc gcgcgccatt cctcgcgcgt ccgcgtcttg aatagcgccg
     1681 ccattttttc gcgcaaagcc ggccagttgg ccggatcgtc gcggtcaatc gtctcgtcaa
     1741 ggccgatcag cgcgcaaagc cgggcgtaga agcgcggctc cagcgcgccg atcgcgatgt
     1801 gctcaccgtc ggcgcagaca taagtacgat agaacggcgc gccgccgtcg agcaggttgg
     1861 cgccgcgcgc tttgcgccag cggccggccg cgctcatctc ggcgcacatc gccatgagcg
     1921 aggcggcgcc gtcgcaaatc gcgcaatcca ccacttgccc ttcgcccgtc cgccgtgcgg
     1981 cgatgatcgc cgcgagcaat ccggcgacca gatagagcga gccgccgccg aaatcgccga
     2041 gcaggttgag cggcgcgatc ggctgttcgg gcgttccgat cgcggcgagc gcgccggcaa
     2101 gcgcgatgta attgatgtcg tggcccgcgc tgccggcgag cgggcctgtc tgtccccagc
     2161 cggtcatgcg cccatagacg agctttggat tgcgcttcag cgcgacgtcc gggccaagcc
     2221 caaggcgctc catcacgccg ggccgaaagc cttcgatcaa agcgtcggcg ctggcgatca
     2281 atgacagcgc ttcgtcgcgc tgcgccgggt ccttgagatc ggcgatcacg gcggggcggc
     2341 cgcgcgagag aatggcgccg ggccggaacg gcgacggctc cggccggtcg atcgcgatga
     2401 tctcggcgcc catatcggca aggagcatcg cggcgaacgg gccgggaccg atgctcgcga
     2461 attcgaccat ccggacgccg gccagcgggc cgccaatgca gccggcggac gttttctttt
     2521 catccattca gcgccccccg ctttgcaagc aggcgcagcc tagcgccgaa tcggcgccct
     2581 gtcgctctga gcccacgcgc cggagggcga tgcgggaaca tcgctttcgc cacattctcg
     2641 gactcaggac gccgtcattc agccacgttg gcgaaaaagt acgacatgtt tccgccatca
     2701 tcgggcttgc ggaatcgcga cgcagcggca ttcacgtcgc attcagggtt gatcatgctt
     2761 ccttttgctg gattttcgct atgtcggaac gagcgaaaat ccgttttggt ttcttaattc
     2821 aatgagcgcc gctcgctgaa ggagcaacaa gcagtcgcgc ttcgccgatc cgactggaag
     2881 cgagcgctgc gcccttggtg agcaaaggcc aacagaaagg accgccaatg aaaccgaatt
     2941 ctctctcttg cgccgcgcgc gcgtcgttcg cggcggcgct tcttggcgcc gtctgccttg
     3001 tcggctcgcc cgcttttgcg caggaggagg cttgcgcgcc gccgaccgtc gtcgatcagc
     3061 cgccgccgcc tctgccggac tacgatcagc cgccgattcc cgcgccgggc tatatctggt
     3121 cgccgggtta ttgggcgtgg gattcggacg agagcgatta ttactgggtg cctggcacgt
     3181 ggaccgagcc gccgagaccg ggcctgctct ggacgccggg ctattgggcc tgggttgccg
     3241 gcgcctatgt cttccatgcc ggctattggg ggccgcgcgt cggctattac ggcggcattc
     3301 attacggcta tggctatacg ggcgagggct atgagggcgg ccgctgggag cacggcacgt
     3361 tcttctacaa tcgtaccgtc aacaatatcg gcaacgtcac catcccgaac gtttacgaga
     3421 agaagatcgt catcaacaac aacacgacga ccaacatcag ctacaatggc ggcaagggcg
     3481 gcgtcgaggc caagccgacg cccgaagaca agacctatgc gcgccaggcg catttcgcgc
     3541 ccaccccggc gcaacgccag catgtgacca cggcgagcaa ggatcccgcc ttgttcgaga
     3601 aaaccaatca cggcgccccg gcggtcgccg cgacgcctcg cccggcggat tttaaagggc
     3661 ccggcgtcgt cccggcccat gctgcggcga cgcagccggg cgcggcagga aagaagccgg
     3721 aagagcagaa gccggccccc ggcaaacccg agctcgaaca gaaaaagccg gccgccggag
     3781 cggtccagcc cggcgtggag gaaaagggca aaacgccgct gaccgaagag aaaaagccgg
     3841 ccgagggaaa caagccggcg ctcgagcaga agaagccgct gacggaagag aaaaagcaga
     3901 tggaagggaa caaacccgcc gtcgaggaaa agaaaccggc catccaagag aagaagccag
     3961 cgatcgagga gaaaaaaccc gtcatcgagg agaagcggcc gacgatcgaa gagaagaggc
     4021 cggtcgaaga aaagcggccg cagccgacga gaccggaaac gcaaagtcgg cccgagccga
     4081 tgcgcccgca gccgcaagcg cggccggagc ccaaaatgca gatggagaag ccggcgatgc
     4141 ggccgcagcc gccggccggc gcccatccgc ctggcaagcc agaggaaaaa gaaaagaaac
     4201 agccttaatc cgagccgaaa tcgccatgcg gcgcccaatc ggcgccgcat ggcgcggcgg
     4261 gggctttcaa cccgcgccgc cggcgcttat attggctctg tcgccgcaag gcgactatgg
     4321 cgataaacgg acgtcggaat aaacctatcg gacccggggg cggtacccgg cgcctccacc
     4381 aaagcccgtt cgcgaagcgg gtttcggcgg gggcgaaata ggatcgacga gggtgtaaag
     4441 ggcgctcttt tgctcggcat gataccgccg ttatcgggtc aaccttatag ttgccaacga
     4501 caactatgct ccggtggctc tcgccgcgta atgcggtgac gaaactggaa atcaagtcct
     4561 gaagggtagc actttcaggc ggggctcgga ggcgcctggc aacagaagcc tccactttct
     4621 ttttcccgca tcaacgcgcg ttgcaatggc gccgccgctc cgatacttgt tgccgccgag
     4681 ccatgccgac cgacctcatc cgctacgatc ttctggtgca agacgcgttg cgcgccgtgg
     4741 tgcgccgcgt cttgagcgac gccgcgcgca acggcctgcc gggtgagcac catttcaaca
     4801 tttctttcaa aacccaggcg cctggcgtcg cgttgccggc ggcgatccgg caaaaatatc
     4861 cggacgagat gaccatcatc ttgcagcatg aattctggga tttggcggtc gaggccgacg
     4921 ggttcgaagt cagcctgaac ttttcgcgca ggcccgaacg gctgaagatc ccgtttgatg
     4981 cgatcgtcgg attcagcgat ccgtccgtcc cgttcggatt caagctggag ccgcgcgagc
     5041 aggccgcggc gagcgcgccg gcgctcgaaa agcaagagcc gcctcctgct cctctggcca
     5101 agccgcagcc ggcgcccctc ccggcgcccg ccgccaagcc cgccgcgagc ggcgccagcc
     5161 gtccggcggc gggagagacg cgcaagaccc cggaaaaaga tgacgcgacc aaagtcgtct
     5221 cgatcgacgc tttccgcaaa aagtaggttc gggatggggg agatcgtcaa tctccgccgg
     5281 acccgcaagc ggcaagccaa ggccgccgcc gaggacgaag cggccgccaa taggctcgct
     5341 ttcggccgaa gcaaagagga gcgccggcga ctaaaagccg agcgcgaggc gcaggccaag
     5401 cgcctcgatg ggcatcggtt ggataagccc gtcggcaatg gcgagtgacg aggcgcgagg
     5461 cgccgcgatc gtcaagcgct cgatcgccat agccggccat cgcaccagca tttcgctcga
     5521 agacgcgttc tggcgcgagc tcaaagcgct cgcccggcgg cgcggccgct cggtcagcgc
     5581 gctcgtcgcg gccatcgacg cggagcggaa aagcgccaat ctgtcatcgg cgattcgtgt
     5641 cttcgtgctc gaagccctgg cggagcgctg aggagcgctc cgcgccctgt cattcccgcg
     5701 aaaagcggga atccgggagc atttgcgttt gctggataga tcgtactgga ttcccgcttg
     5761 cgcgggaatc gtagaagata ccaggtacga actccgctca ataaatccct gtcggatcga
     5821 gccgacgcgg cggcggcttt gccggcggct gcagcgcggt cggggaggcg agagaattgg
     5881 gcggcgcatc gggcacgggc ggcggcaagg gcggagccgc gagcagcttg cgggcttcct
     5941 cttcggcctt gcgggcgtcg tcggcctttt tcgcttcctc cgcctgtcgc gcttcgtcgg
     6001 tcttccgcgc ctcttccgct tcgcgcgcgt cctcgctcgc cttgagcaag tcgtcctcga
     6061 cgcggcggcg ctcggcttcg gatttcaact gcgcctgatc ggcggcatag gcttgcagct
     6121 ccaactcgcg ttggcgcatg aaatgcgccg ctttcagccg gcgattgaaa aaggcgcgct
     6181 cgcgtacgtc cgcctcgaat tcggcgatgc gctcggtttc gcgcgcgatc gcctgcgctt
     6241 caaggccgtt ggcgaggctc gccgcatcga tgtcgcgggc cagcgcgccg actcggccct
     6301 tcaaggcgac cgtcatcgcc ggcggcggcc cgctccagaa ttgttttggc gcttgcttct
     6361 cgctgatcgc ggcgcggata tcgaggtcga aagagcgcaa atcgaggctc gcttgcactt
     6421 tcgcatcgcc ggtctggtcg gccgccgtaa gcggcccgac gcgcagcacg ccggcgctca
     6481 gcgcggccgg cgcgctttgg tcggcgattt tcatcggctt tcgatccatc tcgactgcga
     6541 gcatgtgatt gatgtcggcg ttttggatgt caaagccgct cgcttgcgcg gcggcgacga
     6601 cgcgggccag cgcgccggga tcgaggcgcg gcaactcggc gccggcgagg tcgatatggc
     6661 ccgagccggc gaggcctgcg acgagcgccg cctggctctc gccggtggcg gcgaaatcga
     6721 tcacgccggc gagcttggcg ttgagcccgg cgcgatcgag cgaaatcgaa tcgagcgcga
     6781 tgcggccggc aatggcggcg gtcgcgccgt cccgacgcaa ggtcgcatga ccgctgaccg
     6841 cgccgccggc aacgcgcatc gaaacatcgt cgagcgctgt cgcggtgggt ccgatcttca
     6901 agcggatgtt ggcgtcatgc gcggcaagtc cgttcatcaa gtcgagcgag ccgatcgtca
     6961 gcgccacgtc ggtcgagggc ggattggcga cgaccggcga gaatttgagg tccgaccatg
     7021 gctggccggc tttggccggc tgcgccgggc caagcgccag cgcggcgagc gcgccgagcg
     7081 gcaggcggtc gagcgtcagg gcgccttgaa tctgcggcgg cgggtcggcc ggaggcgcgc
     7141 ccggcatcgc cgctgcgccg gcctgaggcg gtgcggcctg ataagtcaga gcgccgctga
     7201 tcgctgagcc ggccgcgacg cctttcacgc gcgacagccg cgcctcgccg ccgcgccagg
     7261 tcaggtcggc ggaaaggtcg gcgccggcgc gtccgctcgc ctcgcgcgag acgacgccga
     7321 acgccgccag gagcggcagc agattggcgc tcttcaaagt cagcgcgccg ctcgccgtcg
     7381 ccgtcgggtc gagggcgcgg ccgcgccagg tgaggtcggc gccggcgagc gaggccgtcg
     7441 cctcgccggc gaaaccgcgg ctccagtcgc cttcggcgcg aaggtcgacg gcgccgcggc
     7501 caaggccggt caaaggcggc gcctgcaggc cgaattggcg cagcagaacg gaaacttcag
     7561 gggcgtcgag cgaggcggtc gcgacaagct tgccgccgga atccgccggg cggtcgattt
     7621 tcgcggcgat cttcgttgcg cccatgacgc cgcgtatggc gagcgtatcg ggcgcaaaga
     7681 cgctatcctt cgccgcgccg gtcgcgcgag cctcgaaggt cagcttggcc ggcgacagcg
     7741 cgccggcgcg ttcgaccagc ttctgcgcgg cggcgctcgg cgcaatgcgg cgcaagacca
     7801 aagcgaaatc gcgcaagcgc tgggcgtcga catcgacatt gagccagcgc tcggacggtt
     7861 tcagcgcgcc gtccgcctcg atcgtcgcgc cgccgagatc ggcgatggcg agacggtcga
     7921 gcgttacgtc gtcgccggcc ttggagaggc gcaggttcag cgagccgctg tcgacggctc
     7981 cttcgccgaa acgcgcgaga tgcagcgcgt cggcgtccag cgtgagattg agatcgacat
     8041 tgcgcaagaa atcgccgccg gcggcgaaat tcggcagggc gtcgagatcg agcgaatcgg
     8101 tcttgagatc gaggaacaag cggtcgcgat cggcgcccac ggcgcgggtc caggcgagat
     8161 cgcctttgag cgtcgagcgc gccaggccga gcgtcatgtt gcgcgcgacg aagctggttt
     8221 cggaaaggtc gacgtcgcca gcgagcgagg ccgcgctgta aggcaaagcc gcgccgatcg
     8281 cggcgaggcg gccatgcaga gcatcgtcgc cctgggaaat ccagtcgcgc aagcggtcga
     8341 gatcgccgac gcgaatgtcg agcgcgccct tgaaatggct cgccggtccg agctcgacct
     8401 cgcccgacgc ggcgaggcgg gtttggcctg gcgcgagcgc ttgcacgccg agcgtgaccg
     8461 gcgcgccggg cgccgccgac gcgtcgagct cggcgtcgtt cagcgcgtcg ccgccgagaa
     8521 tgatcccggg cgcatcgatt ttgaccgtaa cgggcagcgg cggcccacga tcgagcccgg
     8581 cattcgatag gaacgcggcg atcgcccggt aggcgcgctc cggcggcgct gaatcctcgc
     8641 ccttgccgcg cagcagcgcg tcgagattga gctccttgcc gccgagcgtg acggcgagtc
     8701 gcggcgaggc gccgaaatgc gcctcggccg agccttgcag ggtcaagccg cgcggctcct
     8761 cgccaaggcg cgcttcgagc tttttcagct tggcggtttc gaaattggcg gaaagatcgc
     8821 cggcgatgcg ccacgacgtc ggcgcggcgc ccgggcgggc gaacgcgccg gaaaagaccg
     8881 cgccgccggc atagccgaaa tcgctcgcgc caagcgcaag cgcgccgtcg aattcggcgc
     8941 gcggcgcggc ggggccgaga tcggcggcta ccttgatcgg cagcgcgcct ttttgcatga
     9001 cgccagtggc gaaatggaag gcgacagcgc gtccgtccgg gcccgcggcg cggcctcgtc
     9061 cccggaaagg gccgcgcaag gaatcggcct gcgcgtcgag gaagacgccg ccgatcgcga
     9121 tcggcggcgc gcccgccgcg ccatcgatga cgatcgagcc gtcatgaagc gcgatcgtgt
     9181 cgaggccgac cgattccggc gcgaccttga cgttgagaag cggcgaggcg atcgcgccgc
     9241 cggcattgcg cttgatctcg atgcgcgggc gctcgaagac cgcgtcggtg aagcggattt
     9301 cgccccggac cagcggcgct agcgagagtt cgagcttcgc ctcctcgcag gaaagatcgg
     9361 cgccgccatc c
//
LOCUS       DATGJW010000040         6875 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_525539, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000040 DATGJW010000000
VERSION     DATGJW010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6875)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6875)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6875
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_525539"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(373..726)
                     /locus_tag="VKU03_01170"
     CDS             complement(373..726)
                     /locus_tag="VKU03_01170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69894.1"
                     /translation="MTQTDPAALESEINRLAAESLALRAILSHVLAELGTIANPDIAA
                     AIRRALAGAERTLAARGTRETERLTPSGLAVALGAVQSMKKAALAAAKPAAGFKEEPG
                     AFHAESNDRWPEDDY"
     gene            complement(779..2506)
                     /locus_tag="VKU03_01175"
     CDS             complement(779..2506)
                     /locus_tag="VKU03_01175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667806.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tannase/feruloyl esterase family alpha/beta
                     hydrolase"
                     /protein_id="HLJ69895.1"
                     /translation="MRSLVLLASVSFCALRALPAFAGPSPDQVAARAKPIACAELTTD
                     GLGLANVTIDSASEAPAANGLPAACIVKGAADRRTGIDGKPYALDFEIRLPSQWNGRF
                     LHQVNGGNDGAVVPAIGDPQELNAYGGKPALARGFAVLSSDEGHNGADPVNASFGLGA
                     GVAFAADPEARDDYGYRGDMILGPIGKAIVARYYGEAPARSYMFGCSNGGRHAMVAAT
                     RMGDQYDGFVAGDPGFNLPRAAIQHAWDAQSFERIDPDIKKSFSPADMALIAHKVLAA
                     CDKLDGVEDGMVGDIKACQKVFHLADLQCAGEKTDQCLSTVQVEALTRAFGGPHNSKG
                     EQLYVDWPFDGGMSSANWRFWKVFSGVPPWHGNPLIATMGAASLAAIFTTPPTFTKGD
                     PDSLMAFLSHFDFDRDAPKIYAKGTFTVDGKPVEYKESAWEFMTPPDADDPKLATLRA
                     SGHKIILYHGQSDGVFSFNATADWMDKLDANSGGDAGAFARLFAVPGMNHCSKGPATD
                     NFDMLQAIVDWAENGKAPDRIIAGVTPGNKEIPADWSPQRTRPLCPWPKVARYIGGDK
                     EKAESFACR"
     gene            2705..3856
                     /locus_tag="VKU03_01180"
     CDS             2705..3856
                     /locus_tag="VKU03_01180"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HLJ69896.1"
                     /translation="MRIATSVFSVLVLAAAGAAYGLAAEDAPLLTGAAAYGDWRADAP
                     GVRRKITPADMPPPLASRPVAEPPRIVARPDGATPKAPRGFAVSLFAKGLAEPRTIRV
                     APNGDVFVAESVAGRVRVLRAKAGAEEAADSQVFASGLNRPFGIAFYPPGLNPSFVYV
                     ATSDRIVRYPYHGALAAGPAEPIATLPRGEYHWTRDLVFSPDGETMYVSVGSASNVAE
                     EVATPPAAELKRFVESHPLGAIWGDEEERADILAFDPDGRNKRIFATGLRNCSGETIQ
                     PETGALWCATNERDLLGDDLPPDYATSVKEGAFYGWPWYYIGDHPDPNRANERPDLAG
                     KVTVPDVLIQPHSAPLGITFYEGAQFPAAYKGDAFVALHGSWNRAKRRL"
     gene            3861..4046
                     /locus_tag="VKU03_01185"
     CDS             3861..4046
                     /locus_tag="VKU03_01185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69897.1"
                     /translation="MRLIFQDGKPTGEYDDFLTGFVASDAAVWGRPVAVAVAADGALL
                     VTDDAGGAIWRVAYQGK"
     gene            4358..4636
                     /locus_tag="VKU03_01190"
     CDS             4358..4636
                     /locus_tag="VKU03_01190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metal/formaldehyde-sensitive transcriptional
                     repressor"
                     /protein_id="HLJ69898.1"
                     /translation="MSHTIHHKKKLLARIRRIRGQVEAIERALDSEAGCEKVMHLIAG
                     ARGAIAGLMSEVVEDHVRTHLVDAKRHPEALNGDAAEQLLDVVRTYLR"
     gene            4642..5880
                     /gene="dmeF"
                     /locus_tag="VKU03_01195"
     CDS             4642..5880
                     /gene="dmeF"
                     /locus_tag="VKU03_01195"
                     /inference="COORDINATES: protein motif:HMM:NF033827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDF family Co(II)/Ni(II) efflux transporter
                     DmeF"
                     /protein_id="HLJ69899.1"
                     /translation="MTSSDRRGPSAPHSHIFLGEGHERSERRAWAVIGLCGAMMAAEI
                     VGGLLFGSIALVADGLHMSTHAGALLLAALAYRYARRHAEDPRFSFGVGKLGDLAGFA
                     SAIILSMIALLIGYEAVQRFFRPVPIDFAEAIPIACLGLAVNVASVLILGGGHAHGHA
                     HRHLDPHAGDEGEESNAAAGAEAPALEIFEDGAPPRFRLRAAAAPLPAPQAVSVETVR
                     PDGARQTFLFADRGGFLESIDEIPEPHEFAAHLRVAGAERRLVFEEHSHAVGAAARDN
                     NMRAALVHVLADATVSVLVIAGLVLARAFDWLWIDPLVGMIGAFVIASWSYGLIRDTG
                     AILLDMSADRDLETRLRQVVESEGDTLADLHLWRLGPGHLGAILSIRTVQRRDADYYQ
                     ARLARFKALSHLTVEVKKAA"
     gene            6011..6400
                     /locus_tag="VKU03_01200"
     CDS             6011..6400
                     /locus_tag="VKU03_01200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69900.1"
                     /translation="MPNGDIALGLAGLQEWSEPVIFDRLLDIYRDCLGVGVSAWSLAR
                     LHASVWRAAIKGDIEKFEAARAALTSALSEHPVGGDDVAAIDAEILTELLDIVMARFQ
                     RSSGAARAYHLALIALAGRLTPQPAAA"
BASE COUNT         1073 a         2311 c         2305 g         1186 t
ORIGIN      
        1 ccctcccgct tgacgggtct gaacggcagg atgtcggctt tggcggcggg gcgcatggaa
       61 ttcccctcga gaggagttgc gccgatcaac gcgcgaagga attgcggggt tccggcagca
      121 aagcagacgc gccgagtacg ctggggtccg cataaaattc ggcctcgccg ggctgccagc
      181 gaggccgaaa gttggcgcac cgcgggccag cgggggaggg gcgcagcccg cgcgcctggc
      241 gccgtaaccc tggcgcgcag cgaaggttcg cggcggcggt tcacagaatc gcgaccgcga
      301 cgggccgtac ctcgccgcgc gccggggaag cagcgctaac gcgtccagtc tatcgctgtt
      361 ggcgcggccg gatcaataat cgtcctccgg ccatcggtcg ttcgattcgg cgtgaaaggc
      421 gccgggctct tccttaaatc cggccgcggg cttggcggcg gccagcgccg cttttttcat
      481 cgattggacg gcgcccagcg ccacggcaag gccggaaggc gtcagccgct ccgtttcgcg
      541 cgtcccgcgc gccgccagcg tccgctcggc cccggcgagg gcgcgccgga tcgcggcggc
      601 gatgtccgga ttggcgatcg tcccgagctc ggcgaggaca tgcgagagaa tggcgcgcag
      661 cgccagcgat tccgccgcca aacggttgat ctcgctctca agcgccgccg gatcggtttg
      721 cgtcatcgct cgctcccgcc attcgcctcg atctgggaag gcggccgcgc cgccttgctc
      781 aacggcaggc gaagctctcc gccttctcct tgtcgccgcc gatatagcgc gcgactttcg
      841 gccatgggca gagcgggcgc gtgcgttgcg ggctccaatc ggcggggatt tccttgttgc
      901 ccggcgtcac gccggcgatg atccgatccg gcgccttgcc gttctcggcc caatcgacga
      961 tcgcctgcag catgtcgaaa ttgtcggtcg ccggtccttt cgagcaatgg ttcatgccgg
     1021 gcacggcgaa gaggcgggcg aaggcgccgg cgtcgccgcc ggaattggcg tcgagcttgt
     1081 ccatccaatc ggccgtggcg ttgaaggaga aaacgccgtc gctctggccg tggtagagga
     1141 tgatcttgtg gccgctcgcc cgcagcgtcg cgagcttcgg atcgtcggcg tcgggcggcg
     1201 tcatgaactc ccaggccgat tctttgtact cgaccggctt gccgtccacc gtgaacgtcc
     1261 ccttggcgta gattttcggc gcgtcgcgat cgaaatcgaa atggctcagg aaagccatca
     1321 gcgaatccgg atcgcctttg gtgaaagtcg gcggtgtggt gaagatcgcg gcgagcgagg
     1381 ctgcgcccat tgtcgcgatc agcggattgc catgccaggg cggaacgccg gagaagactt
     1441 tccagaagcg ccaattggcc gagctcatgc cgccgtcgaa cggccaatcg acatagagct
     1501 gctcgccctt gctgttatgc gggccgccaa aagcgcgggt cagcgcctcg acctgtactg
     1561 tcgagaggca ttggtcggtc ttttcgccag cgcattgaag atcggcgaga tggaagactt
     1621 tctggcaggc tttgatgtcc ccgaccatgc cgtcttcgac gccgtcgagc ttgtcgcagg
     1681 cggccaggac cttatgggcg atcagcgcca tatcggccgg cgagaagctc ttcttgatgt
     1741 cgggatcgat cctttcgaag ctctgcgcgt cccaggcgtg ctggatcgcg gcgcggggca
     1801 ggttgaagcc gggatcgccg gcgacaaagc cgtcgtactg atcgcccatc cgcgtcgccg
     1861 cgaccatggc gtggcgaccg ccgttggagc agccgaacat gtaggagcgc gccggcgcct
     1921 cgccataata tctggcgacg atcgccttgc cgatcgggcc gagaatcatg tcgccgcgat
     1981 agccgtaatc gtcgcgcgct tcgggatcgg cggcgaaggc gacgccggcg ccgaggccga
     2041 acgaggcgtt gaccggatcg gcgccgttat ggccttcgtc gctgctcaac accgcgaagc
     2101 cgcgcgccaa agccggcttg ccgccatagg cgttgagctc ctgcggatcg ccgatcgccg
     2161 gaacgacggc gccgtcattg ccgccattga cctggtgcag gaagcggcca ttccattgcg
     2221 agggcagccg tatctcgaaa tcgagcgcgt aaggcttgcc gtcgatcccg gtccgccgat
     2281 cggccgcgcc tttgacgatg caggcggccg gcaagccgtt cgccgccggc gcttcgctgg
     2341 ccgaatcgat cgttacattg gcgaggccta gcccatcggt cgtgagttcg gcgcaggcga
     2401 tcggcttggc gcgagccgcg acctgatcgg ggcttgggcc ggcgaaagcg ggcagggcgc
     2461 gtagcgcgca aaacgagacg ctcgccagaa gaacaaggct ccgcatgacg tcctccctgt
     2521 tccggcgcgt tcgcccgcgc cggatgagaa ttgttatgct acataactga ttgacgcaag
     2581 cgcggtttca atgttttcgt cattccttgt tacatctccg gaaaagcggg cggcgcggac
     2641 tcaacaggcc cagccgaggg cctctaacgt cgcgggcgcc atcctccgga gcctcccaac
     2701 ggccatgcga atcgcaacga gcgtcttttc cgttttggtc ctggccgcgg ccggcgcggc
     2761 ttacggcctt gccgcggaag acgcgccgct gctgaccggc gccgccgcct atggcgattg
     2821 gcgcgccgac gcgccgggcg tgcgccgcaa gatcacgccg gccgacatgc cgccgccttt
     2881 ggcgagccgg cccgtcgccg agccgccgcg tatcgtcgcg cgccccgacg gcgccacgcc
     2941 gaaagcgccg cgcggctttg ccgtctcgct attcgccaaa ggcctcgccg agccgcggac
     3001 catccgcgtc gcgccgaacg gcgacgtatt cgtcgccgaa agcgtcgcgg gccgcgtgcg
     3061 cgtcctccgc gccaaggccg gcgccgagga agcggcggat tcgcaagtct tcgcttccgg
     3121 ccttaatcgt cccttcggca tcgccttcta cccgcccggg ctcaatccaa gcttcgtcta
     3181 tgtcgcgacg agcgaccgaa tcgtccgtta tccgtaccac ggcgcgctcg ccgccggccc
     3241 ggccgagccg atcgcgacct tgccgcgcgg cgagtaccat tggacgcgcg atctcgtctt
     3301 ctcgccggac ggcgagacga tgtacgtctc ggtcggatcg gcctcgaatg tcgcggaaga
     3361 ggtcgcgacg ccgcccgccg ccgagctgaa gcgctttgtc gagtctcatc cgctcggcgc
     3421 gatctggggc gatgaggagg agcgcgccga catcctcgct ttcgatccgg acggccgcaa
     3481 caagcggatc tttgcgacgg gcctgcgcaa ttgctccggc gagacgatcc agccggagac
     3541 cggcgcgctg tggtgcgcga ccaacgagcg cgatctgctc ggcgacgatc tgccgccgga
     3601 ctatgcgacc agcgtgaaag aaggcgcgtt ctatggctgg ccctggtact atatcggcga
     3661 tcatcctgat ccgaaccgtg cgaacgagcg gcccgatctc gccggcaagg tcacggttcc
     3721 cgacgtgttg atccagccgc attcggcgcc gctcggcata accttctacg aaggcgcgca
     3781 atttcccgcc gcatacaaag gcgacgcgtt cgtcgccctg cacggctcct ggaaccgcgc
     3841 caagcgccgg ctataaagtc gtgcgcctca tcttccagga cggcaagccg accggcgaat
     3901 acgacgactt cctcactggc ttcgtggcga gcgacgccgc ggtctggggg cggcccgtcg
     3961 ccgtcgccgt cgccgccgac ggcgccttgc tggtcaccga cgacgccggc ggcgcgatct
     4021 ggcgcgtggc gtatcagggg aagtaaggaa cttgcgccct cgaggccgtc attgcgcgcg
     4081 aagcgaggca atccattcgt agggcgggcg tataactgga ttgcttcgtc gctccgctcc
     4141 tcgcaatgac gaataggggc tatcggctct tctcgaacca atgatgcgcg tgtaaccggg
     4201 gcaaatttcc cggcgccgcc gtccttgtag ccgcggtcgc cgaccgcggc gggcgcgaag
     4261 cgccctgttt cacgcgaccc ggtcagcgac cgcgcctgca tgcccgcccg gaagcgttgc
     4321 gcgtccccgc cagatagggt aggggcctat ccctaaaatg tcacacacca tccatcacaa
     4381 gaagaagctt ctcgcccgga ttcgccgcat ccgcggccag gtcgaggcga tcgagcgcgc
     4441 gctggatagc gaagcgggat gcgagaaggt catgcacctg atcgccggcg cgcgcggcgc
     4501 catcgccggc ctgatgtcgg aggtcgtcga ggaccatgtg cgcacgcatc tcgtcgacgc
     4561 caagcgccat cccgaggcgc tgaacggcga cgcagcggag caattgttgg atgtcgtgcg
     4621 gacctatctc aggtagcggc gatgacgagc agcgatcggc ggggcccttc ggcgccgcat
     4681 agccacatct ttctcggcga aggccacgag cgaagcgagc gccgcgcctg ggcggtgatc
     4741 gggctctgcg gcgcgatgat ggccgccgaa atcgtcggcg gattgctgtt cggctcgatc
     4801 gccctcgtcg ccgacggcct ccatatgagc acgcatgccg gcgcgctgct gctcgccgcc
     4861 ttggcctatc gctacgcccg ccgccacgcc gaggacccgc gcttttcttt cggcgtcggc
     4921 aagctcggcg atctcgccgg cttcgccagc gcgatcatct tgagcatgat cgcgctgctg
     4981 atcggttacg aagccgtgca gcgcttcttc aggccggtcc cgatcgactt cgccgaagcc
     5041 attccgatcg cttgcctcgg cctggcggtg aatgtcgcca gcgtcctcat actcggcggc
     5101 ggccatgcgc atggccatgc tcatcgccat cttgatccgc atgccgggga cgagggcgag
     5161 gaaagcaacg ccgccgccgg ggccgaggcg ccggcgctcg aaatcttcga agacggcgcg
     5221 ccgccgcgct tccgcctgcg cgccgcggcg gcgcccctgc ctgcgccgca agccgtctcg
     5281 gtcgaaaccg tgcggccgga cggcgcgcgc cagacctttc ttttcgccga tcgcggcggc
     5341 tttctcgaat cgatcgacga aattcccgag ccgcacgaat ttgccgccca tttgcgcgtc
     5401 gccggcgcgg agcgtcgcct cgttttcgag gaacatagcc atgcggttgg cgcggcggcg
     5461 cgcgacaaca atatgcgcgc ggctctggtc catgtcctcg ccgacgccac ggtctccgtc
     5521 ctggtcattg ccgggcttgt cctcgcccgc gctttcgatt ggctgtggat cgatccgctc
     5581 gtcggcatga tcggcgcctt cgtcatcgcg agttggtcgt acggcctcat ccgcgacacc
     5641 ggcgcgatcc tgctcgacat gtcggcggat cgcgacctgg agacgcgcct gcgccaggtc
     5701 gtcgagagcg agggcgatac gctcgccgac ttgcatctgt ggcggctcgg cccgggccat
     5761 ctcggcgcga tcctttcgat caggaccgtc cagcgccgcg acgcggatta ttaccaggcg
     5821 cgattggcgc ggttcaaggc gctgtcccat ctcaccgtgg aggtgaagaa ggcggcgtag
     5881 ggcggcgatt cccagatatt tgcatcgccg gagcattctc tcttgcggaa cggcggaagg
     5941 ctcgattacc atcggcgcgt tgcgagattc ggtttgcgcg ttcacgcgaa ggcaacgtga
     6001 gacagcgaac atgccgaacg gcgacatagc cctggggctg gccgggctgc aggaatggtc
     6061 ggagcccgtc atcttcgacc ggcttctcga tatctatcgc gattgtctcg gcgtcggcgt
     6121 ttccgcctgg agcttggcgc gcctgcatgc cagcgtatgg cgcgcggcga tcaagggcga
     6181 catcgagaaa ttcgaggcgg cgcgcgcggc cttgacgagc gcgctgagcg aacatccggt
     6241 cggcggcgac gacgtcgccg cgatcgacgc cgagatcctc accgaattgc tcgatatcgt
     6301 gatggcgcgc tttcagcgct cgagcggcgc ggcccgcgcc tatcacctgg cgctgatcgc
     6361 cttggccggc cggctgacgc cgcagccggc cgcggcgtag cgcaggtcgt acccttttcc
     6421 cgcgcagcgc gctgcacggg gagaaggtgg cgcgaagcgc cggatgaggg gcggcgcagg
     6481 actcgaaggg tacgcgctct acgaaacgct catcaccgag ctcagcgctg ccccgctgcc
     6541 atcgacgcgg aggcgcgtca aggtcaagcc gcttcgcggt cgctccgctt cgcttcgcga
     6601 accttgacac gcctccgcgt cgatggccgt ttgccaccaa gacgttttgc tgctcgaaac
     6661 ggccgaatgg ccggtttcga cagcaaaacg tcttggtagc caataagcca cgccgaccga
     6721 tcgcagtcac ccgaagggcc ggagcgcagc ggaggacccg ccatagcgcc tcgcaagtgg
     6781 atgtccgtct tgcgcgtacc ttggcggcgc tatggcgggt tgacggcgat cgggcggcgt
     6841 ggcgccacag cgctggaagt taagtcgcca ttcag
//
LOCUS       DATGJW010000041         3548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_940225, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000041 DATGJW010000000
VERSION     DATGJW010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3548
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_940225"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1640
                     /locus_tag="VKU03_01205"
     CDS             <1..1640
                     /locus_tag="VKU03_01205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012561235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HLJ69901.1"
                     /translation="VAGCAIAILFFRRVSFMIAAAAPPLLAILLALGALGWLDFRLNM
                     FLNVMTPLIMVISFSDSMQLTFAARDRLIAGEDRFAAFRNAVWIVGPACVLTHATAGL
                     SFIALQFSDSDLIKAFGQAGLIATIIALIAVLTLAPALGVLLVRREAAFAVKIRGADT
                     GVDALRRFCLWVAHRMVSRPGLYSLISLLVVAALALNYLNLQPRYRLADQVPDRQQAV
                     KASNQLDAKLAGANPIDVLITFPKGASLYAPDTLATIAEVHATLESQKGVANVWSLET
                     LRRWLAEKLGKTDVETLKQYVNALPDYLVRRFIAADKSAVVVTGRVPDKDASELLPIV
                     NQLDKRLDTVRKSHPGYQIAVTGLSAIAARSSADMIGKLSRGLTIEFLFVAAFIGLAF
                     RSLPVALAAFLPGIFPIVSAGALLALLGEGLQFASVVALTVSFGLGLSATIHFLNRMR
                     RERRAGADPAIAVERATVLIGPALIVTSIVLACGLAVTAFSDLPTLRLFGWLSAFAML
                     MALTADLLILRPTITFLLRFAARLKGATPLQKGEGQG"
     gene            complement(1867..2367)
                     /locus_tag="VKU03_01210"
     CDS             complement(1867..2367)
                     /locus_tag="VKU03_01210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69902.1"
                     /translation="MSLQFVMRMKACLSGAALVASLAAAPAFAEDGPFTQFAGPWAGN
                     GVITASNGVSERIRCRVHYYVAQQGRDLNQQLRCASDSYHFDVNSALLDVGAGNIVGN
                     WTETNRNAVGKVRGQVDGNVVNAEVVGTGFTAALTVALRGDRQSVKIVPTGSDIASVT
                     IDLRRQ"
     gene            complement(2509..>3548)
                     /locus_tag="VKU03_01215"
     CDS             complement(2509..>3548)
                     /locus_tag="VKU03_01215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HLJ69903.1"
                     /translation="KPLIYALAFEDGLAHPETILFDRPARYGAYAPENFDLGYQGTVT
                     ARYALQQSLNLPAIELLADIGPATFLARLRSAGAEITLPKDAPIGLAIGLGGLGVTLT
                     DIARLYVGLARGGEAPPLVERLDAPRTGEPRRVTSPVAAYYVDDILRGAPPPANALSG
                     RIAYKTGTSYGFRDALAIGFDKGTTIAVWTGRPDNGPTPGLFGRQAAAPILFDAFARL
                     GRDIELILPPKGVLRAATTADLPPPLRHLRKDAPKNIAATAIPALKIAFPPDGARVDL
                     GLKEGASDSHLALKALGGAPPLTWFVNGAPISTPEARRQTAWRPDGAGFARISVIDAK
                     GATDSVVVRLE"
BASE COUNT          566 a         1171 c         1149 g          662 t
ORIGIN      
        1 tcgtcgccgg ctgcgcgatc gcgatcctgt tcttccgccg cgtctcgttc atgatcgcgg
       61 cagcggcgcc gccgctgctc gccatccttc tcgcgcttgg cgcgctcggc tggctcgatt
      121 tccggctcaa tatgtttctc aacgtgatga cgccgctgat catggtcatc agcttttcgg
      181 acagcatgca gctcaccttt gcggcgcgcg accggctgat tgccggcgaa gaccgtttcg
      241 cggcgttccg caacgcggtg tggatcgtcg gcccggcctg cgtgttgacc catgcgacgg
      301 cgggcctctc cttcatcgct ctgcaatttt ccgattcgga tttgatcaag gccttcggcc
      361 aagcggggtt gatcgcgaca atcatcgcct tgatcgccgt gctgacgctg gcgccagctt
      421 taggcgtctt gctggtccgc cgcgaagccg ctttcgccgt gaaaatcaga ggcgccgata
      481 ccggcgtcga cgcgctgcgg cgcttctgtc tttgggtcgc ccaccgcatg gtcagccggc
      541 cgggccttta cagcctgatc agccttctgg tggttgcggc gcttgcgctc aactatctca
      601 acctccagcc acgctaccgc ttggccgacc aggtgccgga tagacagcag gcggtcaagg
      661 cgagcaacca gctcgacgcc aagctcgccg gcgccaatcc gatcgatgtg ttgatcacct
      721 ttccgaaagg cgcttcgctc tatgcgcccg atacgctggc gacgatcgcc gaggttcatg
      781 cgacgctgga atctcagaag ggcgtcgcca atgtctggtc gctcgaaacc ttgcggcgct
      841 ggctcgccga gaagctcggc aagaccgatg tcgagacgct caagcaatat gtcaacgcac
      901 tgcccgacta cctggtgcgg cggtttatcg ccgctgacaa aagcgcggtg gtcgtgaccg
      961 gccgcgtgcc tgacaaggac gcgagcgagc tactgccgat cgtcaatcag ctcgacaaga
     1021 ggctcgacac ggtgcgcaag agccatcccg gctaccagat cgcggtcacc ggcttgtcgg
     1081 cgatcgcggc gcgcagcagc gccgacatga tcggcaagct gagccgtgga ctgacaatcg
     1141 agtttctgtt cgtcgccgcc tttatcggcc tcgccttccg ctcgttgccg gtggcgctcg
     1201 ccgccttcct gccggggatt ttcccgatcg tcagcgccgg cgcgctgctg gcgcttctcg
     1261 gcgagggact gcaattcgcc agcgtggtcg ctctgaccgt gtcgttcggc ctcggcctga
     1321 gcgcgacgat ccatttcctc aaccggatgc ggcgcgagcg gcgcgcgggc gccgatccgg
     1381 cgatcgcggt cgagcgggcg acggtgctga tcggcccggc gctgatcgtc acctcgatcg
     1441 tcctcgcttg cggcctcgcc gtgaccgctt tttccgactt gccgacgctg cgccttttcg
     1501 gatggctcag cgctttcgcc atgctcatgg cgctgaccgc cgatctcttg atcctgcgtc
     1561 cgacgatcac gtttttgctt cgcttcgcgg cgcgattgaa aggcgcaacg cctctccaga
     1621 agggagaggg gcaggggtga gggatacggc gccgaagagt acgatcctgc ctcgctgacg
     1681 tacctctcag cgccgcgcct ttcctccccc gcgagagcgg gggaggggga ccgcgcgtca
     1741 gcgcggtgga gggggcgccg gtctttcagt ataattctca accaccgcgg ccccctccac
     1801 catgcttcgt atggtccccc tcccccgttg cgcttcgctc cacgggggag gatacgccgc
     1861 ggcgagttac tgccggcgca ggtcgatcgt cacagaggcg atatcgctgc cggtcggtac
     1921 gatcttcacc gattgccggt cgccgcgcaa cgccacggtc aaggcggcgg tgaagccggt
     1981 tccgaccacc tcggcgttga cgacgttgcc gtcgacctgg ccgcgcacct tgccgaccgc
     2041 gttgcgattg gtctcagtcc aattgccgac aatgtttccc gcgccgacat cgagcagggc
     2101 gctgttcacg tcgaaatggt agctgtcgct agcgcagcgc aattgctgat tgaggtcgcg
     2161 gccctgctgg gcgacgtaat agtgaacccg gcagcggatg cgctcgctta ccccgttcga
     2221 tgccgtgatg acgccgtttc ccgcccaagg gccggcgaac tgcgtgaaag gtccgtcctc
     2281 ggcaaaagcg ggcgcggccg cgaggctcgc aaccaaagcc gcgccgctca agcaagcttt
     2341 catccgcatg acgaattgta gcgacatgga gacaccgccc ttcgttattt caccggtcga
     2401 gctcataatg taggcgccgc gccgaagaat gacagccgcc gccgcttttc actgtcgatt
     2461 ttttcgattg gctcggccta gcgcgttgaa aagaaaagag actgtctctt actcgagccg
     2521 caccacaacc gaatcggtcg cgcctttcgc gtcgatcacc gagatgcggg cgaagcccgc
     2581 gccgtccggc cgccaggcgg tttggcggcg cgcttcgggc gtggagatcg gcgcgccatt
     2641 gacgaaccag gtgagcggcg gcgcgccgcc gagcgccttc agcgccaaat ggctatcgct
     2701 cgcgccctcc ttcaagccaa gatcgacgcg cgcgccgtcg ggcggaaagg cgattttcag
     2761 cgctggaatc gccgtggccg cgatattctt cggcgcgtcc ttgcgcaaat ggcgcaaagg
     2821 cggcggcaga tccgccgtgg tcgcggcgcg caacacgcct ttcggcggca agatcaattc
     2881 gatatcgcgc cccagcctgg cgaaagcgtc gaacaggatc ggcgccgcgg cttgccggcc
     2941 gaacaggccg ggcgtcggcc cattgtccgg ccggccggtc cagaccgcga tggtcgtacc
     3001 tttgtcgaag ccgatcgcca gcgcgtcgcg aaagccgtag gacgtgccgg ttttgtaggc
     3061 gatgcggcct gagagagcgt tggccggcgg tggcgcgcca cgcaaaatgt cgtcgacata
     3121 ataagccgcg acgggactgg tgacgcggcg cggctcgccg gtacgcggcg catcgagccg
     3181 ctcgacgagc ggcggcgctt cgccgccgcg agcgaggccg acatagaggc gcgcaatatc
     3241 ggtcaaggtc acgccaagcc cgccgagccc gatcgccaag ccgatcggcg cgtccttggg
     3301 caaggtaatt tcggcgccgg cgcttctgag ccgggcgagg aacgtcgccg gtccgatatc
     3361 ggcgagaagt tcgatggccg gcaagttgag cgattgctgc aaggcgtagc gcgccgtcac
     3421 ggtgccttga tagccgagat cgaaattctc cggcgcatag gcgccgtagc gcgccggccg
     3481 gtcgaaaagg atggtctccg gatgcgccaa cccgtcttcg aaggcgagcg cgtagatgag
     3541 gggcttca
//
LOCUS       DATGJW010000042         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_526106, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000042 DATGJW010000000
VERSION     DATGJW010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526106"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..754
                     /gene="lpxD"
                     /locus_tag="VKU03_01220"
     CDS             <1..754
                     /gene="lpxD"
                     /locus_tag="VKU03_01220"
                     /EC_number="2.3.1.191"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="UDP-3-O-(3-hydroxymyristoyl)glucosamine
                     N-acyltransferase"
                     /protein_id="HLJ69904.1"
                     /translation="AVAEVLRRLYPSASRPGSAFGEAGISPSASVHPSAQLEAGIVVD
                     PGAVIGPGAEIGAGTVIGANAVIGPNVRIGRDGAVGAGATVFCALIGNRVIIHPGVHI
                     GQDGFGFAMGPRGHTKVAQVGRVIIQDDVEIGAGSAIDRGANRDTVIGEGTKIDNLVQ
                     VAHNVVIGRHCVLAAQVGVSGSCTLEDFVAIGGQAGLAGHLHIGAGAQIAAAAGLMED
                     VPAGGRWVGMPARPAREWWKDVANIKKLRDGL"
     gene            948..1415
                     /gene="fabZ"
                     /locus_tag="VKU03_01225"
     CDS             948..1415
                     /gene="fabZ"
                     /locus_tag="VKU03_01225"
                     /EC_number="4.2.1.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-ACP dehydratase FabZ"
                     /protein_id="HLJ69905.1"
                     /translation="MDQAATRTFESYDIQKLLTLLPHRYPFLLVDRIIEAKGDESCIG
                     IKNVTANEPQFTGHFPGRPIMPGVLLIEAMAQTAGALCVASLGGAIRSPSVYLMTVDK
                     AKFRKPVVPGDRVEFHMTKFAKRRNIWWFRGEAKVDGALVCEAEVSAMLVAAE"
     gene            1423..2262
                     /gene="lpxA"
                     /locus_tag="VKU03_01230"
     CDS             1423..2262
                     /gene="lpxA"
                     /locus_tag="VKU03_01230"
                     /EC_number="2.3.1.129"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008780 -
                     acyl-[acyl-carrier-protein]-UDP-N-acetylglucosamine
                     O-acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-ACP--UDP-N-acetylglucosamine
                     O-acyltransferase"
                     /protein_id="HLJ69906.1"
                     /translation="MALISPMAPTSGAVIHASAIVEPGATIGAGVSIGPFCHVGAGVV
                     LEDGVTLISHVSLAGTTIVGARTKIYPFASIGHPPQDLKYRGEPVRLIIGPDCLIREG
                     VTMNPGTAGGGSETIVGARGVFLANSHVAHDCKLGDDVILSNNVMLAGHCRIGDFAIL
                     GGGAAVHQFARIGAHAFIGGLAGVENDVIPFGIALGNRAALAGLNVVGLRRRGFGREA
                     IKELHRLYRLLFAAEGTLKERVESLIAEFAGQPEAMQILDFLREGGDRAVCMPRPGKD
                     EAV"
     gene            2259..>2861
                     /gene="lpxI"
                     /locus_tag="VKU03_01235"
     CDS             2259..>2861
                     /gene="lpxI"
                     /locus_tag="VKU03_01235"
                     /EC_number="3.6.1.54"
                     /inference="COORDINATES: protein motif:HMM:NF037151.4"
                     /note="LpxI, functionally equivalent to LpxH, replaces it
                     in LPS biosynthesis in a minority of bacteria; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-2,3-diacylglucosamine diphosphatase LpxI"
                     /protein_id="HLJ69907.1"
                     /translation="MTGGTQASQLAAERPLAIICGGGDFPVALAEAALQAGRSPFLIG
                     LAGSADKRIEAFPHLWLRLGEVGKFFDALTERNIVEVAAVGRVRRPELAELRLDFGAV
                     KRLPALTALFAGGDNRLLAGVARLLEREGLRLVGVHQVAPELLAPEGALARRAPSAQA
                     LDDARLGASVIAALSPYDVGQAVVVAKGRVLAIEAAEGTDA"
BASE COUNT          429 a          985 c          989 g          458 t
ORIGIN      
        1 cgcggtcgcc gaagtcttgc gccgcctcta tccgagcgct tcgcgcccgg gttcggcctt
       61 cggcgaggcc ggcatatcgc cgagcgcttc cgtccacccc tccgcgcagc ttgaagccgg
      121 aatcgtcgtc gatcccggcg cggtgatcgg gcccggcgcg gaaatcggcg ccggaacggt
      181 gatcggcgcc aacgccgtca ttggcccgaa cgtccggatc gggcgcgacg gcgccgtcgg
      241 cgccggcgcg acggttttct gcgccctgat cggcaaccgc gtcatcatcc atcccggcgt
      301 ccatatcggc caggacggat tcggcttcgc catggggccg cgcggccaca cgaaagtggc
      361 gcaggtcggc cgcgtcatca ttcaggacga tgtcgagatc ggcgccggct cggcgatcga
      421 ccgcggcgcc aatcgcgaca cggtcatcgg cgaggggacg aaaatcgaca atctggtgca
      481 ggtcgcccac aacgtggtga tcggccggca ttgcgtgctc gccgcgcaag tcggcgtctc
      541 aggctcgtgc acgctggagg acttcgtcgc gatcggcggc caggccgggc ttgccggcca
      601 tctccatatc ggcgccggcg cccagatcgc cgccgcggcc ggcctgatgg aggatgtccc
      661 cgccggcggc cgctgggtcg gcatgccggc gcggccggcg cgcgaatggt ggaaggacgt
      721 cgccaatatc aagaagctcc gcgacgggct gtagccgcgg tcgttgaccg cggcgcgcgg
      781 actgcggcgg cgttggccgc gatcagcgat cgcggctcag gcgactgcgg ctacaggcgg
      841 cggcaggctg gcgcgcggcg acaaaaagca ctatccaatc gcgcatcgcc gccgcaaaaa
      901 tttgcctcgg cggccggctc gcttttcaag gcaatgaggg gacaggaatg gatcaagccg
      961 cgacgcggac gttcgagagc tacgacattc aaaagctgct gacgctcctg ccgcatcgct
     1021 atccgttcct gctcgtcgac cggatcatcg aggccaaggg cgacgaatcc tgcatcggca
     1081 tcaagaacgt caccgccaac gagccgcaat tcacggggca tttccccggg cggccgatca
     1141 tgcccggcgt gttgctgatc gaggccatgg cgcagaccgc cggcgcgctg tgcgtcgcct
     1201 ctttgggcgg agccatccgc tcgccgtcgg tctatctgat gacggtcgac aaggcgaagt
     1261 tccgcaagcc cgtcgtgccg ggcgaccgcg tcgagtttca catgacgaag ttcgccaagc
     1321 ggcggaacat atggtggttc cgcggcgagg cgaaggtcga cggcgctctg gtctgcgaag
     1381 cggaagtctc cgcgatgctg gtcgcggcgg agtaaggcgc gaatggcgtt gatcagcccg
     1441 atggccccga cgagcggcgc cgtcattcac gcgagcgcca ttgtcgagcc gggcgcgacg
     1501 atcggcgccg gcgtctcgat cgggccgttc tgccatgtcg gcgccggcgt cgtgctggag
     1561 gacggcgtaa cgctgatctc ccatgtcagc ctggccggta cgacgatcgt cggcgcccga
     1621 accaaaatct atcccttcgc ctcgatcggc catccgccgc aggatctcaa atatcgcggc
     1681 gagccggtcc gcctgatcat cgggccggac tgcctgatcc gcgaaggcgt gacgatgaat
     1741 cccggcaccg ctggcggcgg ctcggagacg atcgtcggcg cgcgcggcgt gttcctcgcc
     1801 aattcgcatg tcgcgcatga ctgcaagctg ggcgacgacg tcatcctgtc caacaacgtg
     1861 atgctcgccg gccattgccg gatcggcgat ttcgccattc tcggcggcgg cgcggcggtc
     1921 caccaattcg cgcggatcgg cgcgcacgcc ttcatcggcg gcctcgccgg cgtcgagaac
     1981 gacgtcatcc ccttcggcat cgcgctcggc aatcgcgcgg ccctggccgg actcaatgtc
     2041 gtcggcctcc gccggcgcgg cttcggccgc gaggcgatca aggaattgca ccgactgtac
     2101 cgattgctct tcgccgccga aggcacgctg aaagagcgcg tcgagagcct catcgccgaa
     2161 ttcgccggcc agcccgaggc catgcagatc ctcgatttcc ttcgcgaagg cggcgaccgc
     2221 gcggtctgca tgccgcggcc cggcaaggat gaggccgtgt gaccggcggg acgcaagcct
     2281 cgcaactcgc ggccgagcgg ccgctggcga tcatttgcgg cggcggcgat tttccggtcg
     2341 ccttggcgga agccgcgctt caggccggcc gatcgccttt cctcatcggg ctcgccggct
     2401 cggccgacaa gcgcatcgaa gcttttcccc atctctggct gcgccttggc gaggtcggca
     2461 aattcttcga cgccttgacg gagcgcaaca ttgtcgaggt cgccgcagtc gggcgcgtcc
     2521 ggcggcccga actcgcggaa ctgcgtctcg atttcggcgc agtgaagcgc ctgccggcgc
     2581 tcaccgcctt gttcgccggc ggcgacaatc ggttgcttgc cggcgtcgcc aggctcctgg
     2641 agcgggaagg gctcaggctc gtcggcgttc accaggtcgc gccggaactg cttgcgccgg
     2701 aaggcgcgct ggcgcgccgc gccccgtcag cgcaggccct cgacgacgcg cgcctcggcg
     2761 cctccgtcat cgcggcgctg tcgccctacg atgtcggcca ggcggtcgtc gtcgccaagg
     2821 gcagggtgct ggcgatcgag gcggcggaag gcaccgacgc g
//
LOCUS       DATGJW010000043         5064 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_7577, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000043 DATGJW010000000
VERSION     DATGJW010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5064)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5064)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5064
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7577"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..1640)
                     /locus_tag="VKU03_01240"
     CDS             complement(99..1640)
                     /locus_tag="VKU03_01240"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HLJ69908.1"
                     /translation="MRKGWIDRARPGKARSVLALLFAIAAFFALSASPVYAAADRRVA
                     LVIGNAAYKIAPRLDNPVIDAKAVAAKLKSLGFQVFEGYDLNSDQMRSALSQFSAALE
                     GSDAALVYYSGHGVALDGENYLLPTDINPKTPGDLDLNAISATQVLKQMRRDERAIVL
                     ILDACREDPFAAELARTKSRAIIGARGLNPIEGDAARGALIAFATDPNSVAFDGPPGQ
                     HSPFTKALLDHLDDAGVPIETVMSRVRAEVWAATKNKQLPWVNTSIIGEFALNPKPEP
                     AAAASPAPAPASQSTENLLWESAQRSNLAADYKAYLDAFPSGVFAQMARNRIAALSAP
                     DADQSASLSRTATVEADAAEAEKKLNLAPAERRELQLRLAALKFDAGPPDGDFGDKAR
                     AAIREWQKRHDLKPTGYFEAPQQVTMLREESEDAYRRLIAPPIRIQKAPVANAHPEPL
                     REEPRRRQRPSRPAHRPVAEPSRQAAQPAPPPATDQFIGGCQAGFHNVPAPTPSGYRC
                     VQNGY"
     gene            1856..2629
                     /locus_tag="VKU03_01245"
     CDS             1856..2629
                     /locus_tag="VKU03_01245"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006202949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HLJ69909.1"
                     /translation="MAYHTIIVETHGKVGLVRLNRPQALNALNARLIEELGQALKGFE
                     ADSAIGAIVLTGSEKAFAAGADIKEMSGKTYMEAYLENFIGEWQALADTRKPVIAAVA
                     GFALGGGCELAMMCDFIIAAETAKFGQPEIKLGVIPGAGGTQRLARFIGKAKAMDLIL
                     TGRMMDAAEAERCGLVARVVPADKLIEEALKAAATIAGYSLPSVMIAKESVNRAYETT
                     LAEGVRFERRVFHSLFATEDQKEGMAAFVEKRPPSFKNR"
     gene            complement(2738..2977)
                     /locus_tag="VKU03_01250"
     CDS             complement(2738..2977)
                     /locus_tag="VKU03_01250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69910.1"
                     /translation="MAREIGETDGAADFADDPLHFVVGQDGGGNWIVTEAHGLYGGIF
                     CSKDAALRFAKFEKADRLGQCAVTAERIEFDVRRR"
     gene            3207..4811
                     /gene="aceB"
                     /locus_tag="VKU03_01255"
     CDS             3207..4811
                     /gene="aceB"
                     /locus_tag="VKU03_01255"
                     /EC_number="2.3.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004474 - malate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malate synthase A"
                     /protein_id="HLJ69911.1"
                     /translation="MNDSPPETVSGVVLQAQKKARAEEILTPAALAFLADLHRRFDKR
                     RRELLARRAERQKLFDAGETPDFLPETRDIRESDWRVAPIPADLLDRRVEITGPVDRK
                     MIVNALNSGAKVFMADFEDATSPSFANMIEGQINLKDRWDGKIDFVDPTSGKAYKLAA
                     KPAVLMVRPRGWHLPERHLTVDGEVMSGALFDFGLYVLHNAKAQMAQGATPAFYLPKM
                     ESHLEARLWNDVFTFAEERLAIPKGTFKATVLIETLPAAFEMDEILYELRDHMAGLNC
                     GRWDYIFSFIKRLGKNARFLTPDRARMVMGEAFLAAYSLLLIKTCHRRGAFAMGGMAA
                     QIPVKGDAAANEVAFAKVRADKEREATNGHDGTWVAHPDLVPVAAEVFDRVMPQPNQI
                     DRLREDVTVSREDMLRVHEGQRTEAGLRENIRVGVQYIEAWLRGRGAVPLYNLMEDAA
                     TAEISRAQIWQWLRLGATLDDGRQVTPELFRATLADEMNKLRNAFTPAVFESGRFAEA
                     IKLFSDMSLATEFEEFLTLPAYKLID"
BASE COUNT          854 a         1673 c         1656 g          881 t
ORIGIN      
        1 gctcgtcatt gaaccaaaat aagctcgcgt ccatcggctc ggtcccctaa tcttcgacca
       61 agccgttgaa tcaaaacaac cttgtttggg tctggaccct agtacccgtt ctgcacgcag
      121 cgatagccgg agggggtcgg cgccggcacg ttgtggaagc ccgcctggca gccgccgatg
      181 aactggtcgg tcgcgggcgg cggcgccggc tgtgcggctt ggcgggacgg ctccgccacc
      241 gggcgatgcg ccggccggct cggccgctgg cggcggcgcg gctcttcacg caaaggctcg
      301 ggatgggcgt tggcgacggg cgccttttga atgcggatcg gcggggcgat gaggcgccga
      361 taggcgtcct cgctctcttc ccgcagcatc gtcacttgct gcggcgcctc gaaatagccg
      421 gtcggcttaa gatcgtgtcg cttctgccat tcgcggatgg cggcgcgcgc cttgtcgccg
      481 aaatcgccgt ccggcggtcc ggcgtcgaat ttcagcgcgg cgaggcgcaa ttgcaattcg
      541 cggcgctcgg ccggcgcgag attgagcttc ttctcggctt cggcggcgtc ggcttcgacg
      601 gtcgccgtcc gcgaaaggct cgccgactga tcggcgtccg gcgccgagag ggccgcaatg
      661 cggttgcgcg ccatctgcgc gaaaacgccc gacgggaagg cgtcgagata ggccttgtag
      721 tcggcggcga ggttggagcg ctgcgccgat tcccagagaa ggttttccgt cgattggctc
      781 gccggcgccg gcgcgggcga agcggcggcg gccggctcgg gcttcgggtt aagagcgaat
      841 tcgccgatga tcgaggtgtt gacccaggga agctgcttgt tcttcgtcgc cgcccagact
      901 tcggcgcgca cccgcgacat gaccgtctcg atcggcacgc cggcgtcgtc gagatggtcg
      961 agcagcgcct tggtgaacgg gctgtgctgg ccgggcggcc cgtcgaaggc gacgctgttc
     1021 ggatcggtgg cgaaggcgat cagcgcgccg cgcgccgcgt cgccctcgat cgggttgagg
     1081 ccgcgcgcgc cgataatcgc gcggctcttg gtgcgcgcga gttccgccgc gaacgggtcc
     1141 tcgcggcagg cgtcgagaat gagcacgatc gcccgttcgt cgcgacgcat ctgcttcaag
     1201 acttgcgtcg cgctgatcgc gttgaggtcg agatcgcccg gcgtcttcgg gttgatgtcg
     1261 gtcggcagca gatagttctc gccgtcgagc gcgacgccat ggccggaata atagaccaaa
     1321 gccgcgtccg atccctccag cgccgccgag aattgcgaca gcgccgagcg catctggtcg
     1381 ctgttcaaat cgtagccttc gaagacttgg aagccgagac tcttgagctt cgccgcgacg
     1441 gccttggcgt cgatgaccgg attatcgaga cgcggcgcga tcttataagc cgcattgccg
     1501 atgaccagcg cgacgcggcg atcggcggct gcgtaaactg gcgaggccga gagggcgaag
     1561 aaagccgcga tagcaaaaag gagcgcaagg acgctccgcg ctttgcccgg cctcgctctg
     1621 tcgatccaac cctttcgcat gacgcagcgt ccaatcttgc tcgccaagcc ttgtcgaacg
     1681 ggcgatgcta gtccccgccc gccgctcttt caatgcggcg ccgcggacgg agcgtgacgg
     1741 cttggctttt cctttggctg tccgcgacgg ctttgattta cggtccgcga gcggcttgaa
     1801 gcggcttttc attcctgccg ccctcggcta ggccttccgc cgaggagtgt tcgacatggc
     1861 ttaccacacg atcatcgtcg agacgcatgg aaaagtcggc ctggtccgcc tcaatcggcc
     1921 gcaggccctc aacgccttga acgcgcggct gatcgaagag ctcggccagg cgctgaaggg
     1981 cttcgaagcc gattcggcga tcggcgcgat cgtcctgacc ggctccgaaa aggccttcgc
     2041 cgccggcgcc gacatcaagg agatgagcgg caagacctac atggaggcct atctcgaaaa
     2101 ctttatcggc gagtggcaag cgctcgccga tacacgcaag ccggtgatcg cggcggtcgc
     2161 cggcttcgcg ctgggcggcg gctgcgagct cgccatgatg tgcgatttca tcatcgcggc
     2221 ggagacggcg aaattcggcc agcctgagat caagctcggc gtcattcccg gcgccggcgg
     2281 gacgcagcgc ctcgcccggt tcatcggcaa ggccaaggcg atggacctca tcctcaccgg
     2341 ccgcatgatg gacgccgccg aggcggagcg ctgcggcctc gtcgcgcgcg tcgttcctgc
     2401 cgacaagctc atcgaggaag cgctgaaggc cgccgcgacg atcgccggct attcgctgcc
     2461 ttcggtgatg atcgccaagg aaagcgtcaa ccgcgcctat gagacgacgc tcgccgaagg
     2521 cgtgcgcttc gagcggcgcg tcttccattc gctcttcgcc accgaagacc agaaggaagg
     2581 catggccgcc ttcgtcgaga agcggccgcc gagtttcaag aaccggtaga tcggcagtcg
     2641 gcagtaggca gtaggcagtg ggcagtaggt ccaaaagacg accgccattg ccgactgccg
     2701 actgcccact gccgactgcc cactgcccac tgccgaccta tcgtcgccgc acatcgaact
     2761 cgatccgctc ggcggtcacc gcgcattggc cgagccggtc ggccttttcg aatttggcga
     2821 aacgcaaggc ggcgtccttg ctgcagaaaa tgccgccata gaggccatgc gcttcggtga
     2881 cgatccagtt tccgccgcca tcctggccaa cgacgaaatg gagcgggtcg tcggcgaagt
     2941 ccgccgcccc gtccgtctca ccgatctccc gcgccatggc gctgctccct tgctgatctg
     3001 cgctgcgccg cccgggcgcc tttcgcggca taaagcgcgt ttatcggccg caaggtccca
     3061 aacacgacga ttgggcgctc ggctcgcgaa aatgtggctc aaaaacgtta agaaacgcgc
     3121 gccggggatg cgaattgcgc cgaacgcgct tcaaatctgc gatgtcttgc cttaagggga
     3181 ctggccagcc cgaaagagat ggaaaagtga acgattcccc tcccgagacc gtttccggcg
     3241 tcgtccttca agcccagaag aaggcccgcg ccgaggaaat tctcaccccc gccgccctcg
     3301 cttttttggc cgacctccac cgccgcttcg acaaacgccg gcgcgaattg ctggcccgcc
     3361 gcgccgaacg ccagaagctg ttcgacgccg gcgagacgcc ggattttctg cccgagaccc
     3421 gcgacatccg cgagagcgac tggcgggttg cgccgatccc ggccgatctt ctcgaccggc
     3481 gcgtcgagat caccggcccg gtcgaccgca agatgatcgt caacgcgctg aattccggcg
     3541 cgaaagtgtt catggccgat ttcgaggacg cgacctcgcc ctccttcgcc aatatgatcg
     3601 agggccagat caacctcaag gaccgctggg acggcaagat cgacttcgtc gatccgacga
     3661 gcggcaaggc ctacaagctc gccgccaagc cggccgtgct gatggtgcgg ccgcgcggtt
     3721 ggcatctccc ggagcgccat ctcaccgtcg acggcgaggt catgtccggc gcgctgttcg
     3781 atttcggcct ttacgtgctc cacaacgcca aggcgcagat ggcgcaaggc gcgacgcccg
     3841 ccttctacct gccgaagatg gagagccatc tcgaggcgcg gctgtggaac gacgtgttca
     3901 ctttcgccga ggagcgcctg gcgatcccga agggaacgtt caaggcgacc gtcctcatcg
     3961 agacgctgcc cgccgccttc gagatggacg agatccttta cgaattgcgc gatcacatgg
     4021 ccggcctcaa ttgcggccgc tgggactata ttttctcctt catcaagcgg ctcggaaaga
     4081 acgcccgctt cctgaccccg gaccgggcgc ggatggtgat gggcgaggcg tttctcgccg
     4141 cctattccct gctgctcatc aagacctgcc atcgccgcgg cgccttcgcc atgggcggca
     4201 tggcggcgca gattccggtc aagggcgacg ccgcggccaa tgaagtcgcc ttcgccaagg
     4261 ttcgcgcgga caaggagcgc gaggcgacca acggccatga cggcacctgg gtcgcccatc
     4321 ccgatctcgt gcccgtggcg gcggaagtct tcgaccgcgt catgccgcag ccgaaccaga
     4381 tcgaccgcct gcgcgaggac gtgacggttt cgcgcgagga tatgctgcgc gtccatgagg
     4441 gccagcgcac cgaggcgggc ttgcgcgaga acattcgcgt cggcgtgcag tatatcgagg
     4501 cctggctgcg cggccgcggc gcggtgccgc tctacaatct catggaggac gccgcgaccg
     4561 ccgagatttc gcgcgcccag atttggcaat ggctgcgcct cggcgcgacg ctcgacgacg
     4621 gccgccaggt cacgccggag cttttcaggg cgacgctcgc cgacgagatg aacaagctcc
     4681 gcaacgcctt cacgcccgcg gtcttcgaga gcggccgctt cgccgaggcg atcaagctgt
     4741 tctccgacat gtcgctcgcc accgagttcg aggaattcct gaccctcccg gcctataagc
     4801 tgatcgattg agaggcgccg ccttcccctc gcccgcgaag cgcggagagg gcggccgcga
     4861 agcggccggg tgagggctgc gccgagcttg ccgatgagct ttcgtacggc gcttaacctt
     4921 tcgagtcctg cgcagcccct catccggcgc ttcgcgccac cttctccccg caaggcgcag
     4981 ggctgtccgg ggaaagtaac aggcgagtcg catcgttgtc gacgaagttt cgtgaaataa
     5041 cgcgatttcg agatttcctc gttg
//
LOCUS       DATGJW010000044         2473 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1045552, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000044 DATGJW010000000
VERSION     DATGJW010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2473)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2473)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2473
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1045552"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..306)
                     /locus_tag="VKU03_01260"
     CDS             complement(<1..306)
                     /locus_tag="VKU03_01260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ69912.1"
                     /translation="MSLASRNALVTGSTSGIGLAIARALAKNGANIMLNGMGDPAAIE
                     KERAGLEKEFGVKALYNGADMTKPAEIAAMIADAQTKLGSLDILVNNAGIQFVSPIED
                     "
     gene            complement(460..1299)
                     /locus_tag="VKU03_01265"
     CDS             complement(460..1299)
                     /locus_tag="VKU03_01265"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HLJ69913.1"
                     /translation="MAQEPNPKAKRDRRRPAAYHASLEAGEAVHDAASPMKRNANDSL
                     AAALGAEIIAGVYPPGSRLPSESALLDRFKVSRPTLREAFRVLAAKGLIVSRQKVGTS
                     VRPKSDWNMLDPDFLAWHLRAALTEEFVADLFQLRQMVEPQAAYLAAQSRDPEAIERI
                     KSAYADMERWKTGRGDLTGADLRFHQHILDATGNLFIGALGGLIHTALIGTFKLGWSG
                     AVIKDDRLHQHRAVLEAIEAGAPEQARERMAVLLQDSIDDVRRALVKRRATPAGRRSR
                     ELA"
     gene            complement(1319..2284)
                     /gene="yjfF"
                     /locus_tag="VKU03_01270"
     CDS             complement(1319..2284)
                     /gene="yjfF"
                     /locus_tag="VKU03_01270"
                     /EC_number="7.5.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003579213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0103116 - ABC-type D-galactofuranose
                     transporter [Evidence IEA];
                     GO_process: GO:0042875 - D-galactonate transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactofuranose ABC transporter, permease
                     protein YjfF"
                     /protein_id="HLJ69914.1"
                     /translation="MNERYMPLAATIVVFVAIYAFGALAYNNFLSTLVFGDLLTDNAF
                     VIIAAIGATFVILSGGIDLSVGSMIGFVSVAMAALDAHGWHPLLSALLMVSFGALFGG
                     VMGALIDACDIQPFILTLAAMFLLRGLCFVITLDSLPINHPFVGAFSDWRIPLPGGGR
                     LSASALLMLASLAGGIALAHFTRFGANVYAIGGDRNSARLMGAPIRRTMIQVYALGGF
                     YGALAGVAYAFYTGSGYPLAAVGVELDAIAAVVLGGTLLTGGVGYVAGTLFGGLIQGT
                     IQTLITFDGRLTSWWTKIVIGALLFFFITAQRSITRSTALMRRPA"
     gene            complement(2281..>2473)
                     /locus_tag="VKU03_01275"
     CDS             complement(2281..>2473)
                     /locus_tag="VKU03_01275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ69915.1"
                     /translation="RIYIFNTLIGVLIIQSLTTSILVSGLPPEYNLIVKAIVVFFCLL
                     LQAQPVRQAVGGMMWTKRP"
BASE COUNT          493 a          778 c          808 g          394 t
ORIGIN      
        1 gtcttcgatc ggcgagacga actggatgcc ggcattgttg acgagaatgt cgagcgatcc
       61 cagcttcgtc tgcgcgtcgg cgatcattgc cgcgatttcg gccggcttgg tcatgtcggc
      121 gccgttataa agcgccttga cgccgaactc cttctcaagc cccgcccgct ccttctcgat
      181 cgccgccgga tcgcccatgc cgttaagcat gatgttcgcg ccgttcttgg ccaaggcgcg
      241 ggcgatggcg aggccgatgc cgctggtgga gcccgtgacc aacgcgttgc gggaggcgag
      301 agacatggcg aactccttgt aaggcggaga ttgaggcgcg ccgagcatgc gggattgagc
      361 gctcgtgagg ctcatatatt gcgttgcagc aaaaaaggcg agagcggcca tccgcggccg
      421 attgacaggg cggcgttgcc gccgtcgtcg cacccctcgc tatgcgagct cgcgcgagcg
      481 ccgccccgcg ggcgtcgcgc gtctcttgac cagcgcgcgg cgcacgtcgt cgatcgaatc
      541 ctgcaggaga accgccatgc gctcgcgcgc ctgttcgggt gcgccggcct cgatcgcttc
      601 gagcacggcc cggtgctggt gcaagcggtc gtccttgatc accgcgccgc tccagccaag
      661 cttgaacgtg ccgatcagcg ccgtgtgaat aaggccgccc agcgcgccga taaacagatt
      721 gccggtcgcg tcgagaatgt gttggtgaaa ccgcaaatcc gcgccggtca ggtcgccccg
      781 tccggtcttc caacgctcca tatcggcata agccgatttg atccgttcaa tcgcctccgg
      841 atcgcgcgat tgggcggcga gataagccgc ctgcggctcg accatttgcc gcaattgaaa
      901 gaggtcggcg acgaattcct cggtcagcgc ggcgcgcaaa tgccaggcga ggaaatccgg
      961 atcgagcata ttccaatcgg atttcggccg gacgcttgtg ccgaccttct ggcgcgagac
     1021 gatgagcccc ttggcggcga ggacgcgaaa cgcttcgcgc aaggtcggcc gggagacttt
     1081 gaaccgatcg agaagcgcgc tttcgctcgg caggcgcgag ccgggcggat agacgcccgc
     1141 gatgatctcg gcgccaagcg cggcggcgag cgaatcgttg gcgttgcgct tcatcgggct
     1201 ggccgcgtca tggaccgcct cgcctgcctc gagcgacgcg tgataggccg caggccgccg
     1261 tctgtcgcgc ttcgccttcg gatttggctc ctgcgccacc gaaccgctcc ttatgcgcct
     1321 aggccgggcg ccgcatcaaa gccgtcgacc gggtgatcga acgctgagcg gtgatgaaaa
     1381 agaatagaag cgcgccgatc acgattttgg tccaccagct cgtcaatctc ccgtcgaacg
     1441 tgatcagggt ttggatagtg ccctgaataa ggccgccgaa caatgtcccg gcgacgtagc
     1501 cgacgccgcc ggtcaacagc gtgccgccga gcacgaccgc cgcgatggcg tcgagctcca
     1561 cgccgaccgc cgcaagcgga tagcccgacc cggtatagaa ggcgtaggcg acgccggcca
     1621 acgcgccgta aaagccgcca agcgcataga cctgaatcat ggtgcggcgg atcggcgcgc
     1681 ccatcagccg cgctgaattg cgatcgccgc cgattgcgta aacgttggcg ccgaagcggg
     1741 tgaaatgcgc gagcgcgatt ccccccgcca aagaagcgag catcaaaagc gcggaggccg
     1801 acaggcgccc gccgcccggc aaaggaatgc gccagtcgga aaaggcgccg acaaaaggat
     1861 ggttgatcgg cagcgaatcg agcgtgatga cgaagcacag gccgcgcagc aggaacatcg
     1921 ccgccagggt gaggatgaag ggctggatat cgcaggcgtc gatcagcgcc cccatgacgc
     1981 cgccaaacag cgcgccgaac gagaccatca gcaaggccga aaggagcgga tgccagccat
     2041 gcgcgtcgag cgccgccatc gcgacgctga caaagccgat catcgagccg accgagaggt
     2101 cgatgccccc cgacaggatg acgaaggtcg cgccgatcgc cgctataatc acgaacgcat
     2161 tgtcggtgag gagatcgccg aataccaaag tcgacaggaa attgttatag gcgagcgcgc
     2221 cgaaggcgta gatggcgacg aagacgacga tcgtcgccgc caagggcatg tagcgctcat
     2281 tcatggcctt ttcgtccaca tcatgccgcc gacggcttgc cgcaccggct gcgcctgaag
     2341 cagcaggcag aagaagacca cgatcgcctt gacgatgagg ttgtattccg gcggcaagcc
     2401 gctgaccagg atcgaggtcg tcaggctctg gatgatcagg acgccgatca gcgtattgaa
     2461 aatatagatg cgt
//
LOCUS       DATGJW010000045        10695 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_526970, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000045 DATGJW010000000
VERSION     DATGJW010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10695)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10695)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10695
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_526970"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VKU03_01280"
     CDS             <1..1232
                     /locus_tag="VKU03_01280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007634472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ69916.1"
                     /translation="WMTVAENMALAQGFPRRDWLGRFGFIDWRRAEKRADEALALIGC
                     QIDPSRRIRSLSRTEKSLVAIARALMVECDFLVLDEPTASLPADEVERLFDAVRRLRQ
                     NGVGMIYVSHRLDEIFRIADRVVVLRDGRFVGGKPVKETNPDELIGMIVGRSTLDLFH
                     KAEIAAGPDRVVVRNLVTPRAGPVDFEIREGELLGLVGLRGAGQEEIGRALFGAAPHS
                     GSILINGESPELSSPRAALRAEIGLVARDRAEESVATSLSVRENAFLNPGASGRGLFS
                     RLSPAAEARKAVALGASVGLRPNDPDLPIEGLSGGNQQKVIVGRWLATGRRLLIAEDP
                     TAGVDVGAKAEIYRLIERALQEKLAVAVVSTDFEEIAHICHRALVFNRGRIIAELSGP
                     ALTTEAVIAAASASEAA"
     gene            1256..2305
                     /locus_tag="VKU03_01285"
     CDS             1256..2305
                     /locus_tag="VKU03_01285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ69917.1"
                     /translation="MATNSIRSDALEPTRGELKNVSFAMRVTRLLPVYGLPILTIALI
                     ALFSIVLPDTFPTMLNLRSIIADKAIIAILSLAAMITMASGKIDLTVGYGIVIWHILA
                     ISLQSVFGLPWPLAVIIVLLLGAFVGLINGLLVEVAQIDSFIATLGAGTALYAIALWH
                     TGGRQVVANLPHGFLALNGTMIFGLPITGYYVIGLAVILWFVFEHLPVGRYLYAIGAN
                     PKAAELNGIPVRRFTVAAFVASGVLTAVAGVLLASKLRIGQASVGLEYLLPALVGAFL
                     GSTTIKPGRVNVWGTVVGVAILAVGIAGIQQFGGSFWVEPMFNSVTLLVAIGIAGYAQ
                     RRRGAAERQIKKTSA"
     gene            2334..3440
                     /locus_tag="VKU03_01290"
     CDS             2334..3440
                     /locus_tag="VKU03_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ69918.1"
                     /translation="MSRLLLRTAVSGLALALAIGASHADGLVDEAKAIVVKATSRADK
                     WDGPTSGPKAEAKKTVVYVAGDMRNGGILGVAHGVKEAADTIGWTYREIDGQGTVSGQ
                     AAALGQAIALKPDAIIVGGSDAVQQKAGLEDAAKQGITIVGWHAGPKPGPMDGAPVFA
                     NVTTDAMEVAKVAAMKAIADSNGTAGVVVFADSAYAIAIAKGRAMEAEIKKCSGCKVL
                     AFEDTPLADTAGRVPQLTTTLLQQFGAKWTHSLAINDLYYDSMGPSLQVAGLKGDGDP
                     HNISAGDGSESAYQRIRDKDYQSATVPEPLNMQGWQLVDEINRAFAKEKWSGFVPGVH
                     LVTADNIAFDGGPKNVFDPDNGYRDQYKKIWGAK"
     gene            3485..4525
                     /locus_tag="VKU03_01295"
     CDS             3485..4525
                     /locus_tag="VKU03_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003577789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLJ69919.1"
                     /translation="MTDSFEKSGRNQPTMADVAARAGVSAMTVSRALGKGGSIAESTR
                     RRIMAAADELGYVFDQAAGTLSSKRSGFIAVLIPAFDNSNFADTTRGLAEAVESSGLQ
                     VLFGCTDYSLDKEERLIESMLRRRPEGMIVTDGRHTERANRLLRTSGVPVVETWDMPS
                     DPIEHVVGFSNADAMNAVVHSLYQRGYRRIAFIGSASARDTRGGDRERGYRQAVGSLD
                     MPKGRIVSIGEPPMSMTHGCAAIVDVLSRWPDTDAVACLSDLAAFGALMECRRRGWDV
                     PGRVALAGFGDFELSRTAAPRISTVFVDAFAIGRAAGEIMLQAIEAARSGGSLTPQIV
                     AQPFRVLERESA"
     gene            complement(4532..5359)
                     /gene="tam"
                     /locus_tag="VKU03_01300"
     CDS             complement(4532..5359)
                     /gene="tam"
                     /locus_tag="VKU03_01300"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HLJ69920.1"
                     /translation="MTQPIETSERASPFIHANEWDVALYLKFEKERVRAANDLLAHVP
                     TRTPRRVVDLGCGPGASTRLLQTRFGEADVIGLDSSDRMLESARTRLPKINFEKRDIA
                     AWRPTTPPDIIFANSSLQWLPDHHELTPRLYSLLAEGGSLAIQMPDNRQEPSHALMRM
                     VAADGPWTDRLVPIAKTRAVIGAYSDYYRWLRPLGARLEIWQTTYIHALNGVGELVDW
                     FRGSGLRPFLNPLDEAEREQFIARYMEELAAAYPSEPDGCVLFLYPRLFIVATKPGG"
     gene            complement(5506..6420)
                     /locus_tag="VKU03_01305"
     CDS             complement(5506..6420)
                     /locus_tag="VKU03_01305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ69921.1"
                     /translation="MDRLESMSILVTAAEAGSLSAAARRLGVPLATVSRKVSELEAAL
                     KARPLIRSARRLSLTEAGEAYVAACRRILEEVEAAERAAAGEYSEAKGELTLTAPIVF
                     GRLHILPIVTEFLKAYPRIDVRLILADRVMNLLEDRIDLAVRIARLEDSSLLATKVGA
                     IRRVVCASPAYFAARGAPKTPADLDGHDCITFEGLSSPAHWTFALDKKNVATPIHSRL
                     TVTTAEAAIDAAIAGLGLARVFSYQAVAAVRAGALVLALEDFEPPPWPVSLVRGPGGL
                     TPLKLRAFLDFATPRLKERLSQAALSRE"
     gene            6542..7168
                     /locus_tag="VKU03_01310"
     CDS             6542..7168
                     /locus_tag="VKU03_01310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HLJ69922.1"
                     /translation="MSDLRHYSSDIAFTPRVKAIQAQKGSRQAYAEMEEQGAWETRVT
                     PDLAQFIGAQRSVFLATANSEGQPYIQHRGGPPGFLRVIDEKTIGFADFAGNRQYITL
                     GNLADNPKAYLFLIDYTMRRRVKIWGEARIVEDDPALTASLATKGYRARPERALLFTI
                     GAWSVNCPQHIPQRFEAADVAAALAERDQRIADLEAEVKALREAVKAE"
     gene            7745..8569
                     /locus_tag="VKU03_01315"
     CDS             7745..8569
                     /locus_tag="VKU03_01315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69923.1"
                     /translation="MAHLRSAIAGIESQEGPRPRAPFRLPVARALDGALGGGLAGDAL
                     HEIAPAAPGDGAAAMAFSLCLAARFVAASPRRFCLIAYEEFCAREAGALYGPGLAAFG
                     LRRDELVFVKAQDGRALLWTMEEALKSGALAVVVGELWSVAKHYGLAASRRLLLAARA
                     GATPALMIHGGVFGQANALSSAAETRFEIAAAASRRLPPAGGRLALPGAPAFAARLVK
                     SRLSPSAQGPPARLFDETRVERLEWSSAEKSFHDPTVPLPLVRAPLDGPRARVAGK"
     gene            8553..10109
                     /locus_tag="VKU03_01320"
     CDS             8553..10109
                     /locus_tag="VKU03_01320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HLJ69924.1"
                     /translation="MLRENERSAARALTPLATYLKIKNAQQLVCIDRQAASLGLTRGL
                     PLADARARHPNLVAVEADPAEEGKLLARIADWLQRFTPLVALDGADGIMLDIAGVAHL
                     FGGEKAMIQEIETRLAAQGLGARAGIADFPRAAWALARFSKERIAPTGAERKIVAQLY
                     HGLPLAALGLAETNVADMARAGLRRIGDVALRPRAPIAARFGADVIARLDALYGLERS
                     AISPRFAAPEFSAERRFASAIVQKEAVEATLLKLAEDLAALLQRQVKGARRLELALFR
                     VDGAVRRIVVGAGRPLNEPRAMARLFRERFEAPDEDEIDAGYGFDVVRLSCLLAEPLS
                     PAQGDFARAPEEKETRSLADLLDRLSARLGMRRVTRIDLVETHIPEFAVVARPATLGD
                     ARAPFTMLRMVPLPRCAGEEKGGAKQGEGAPPTRPLRLLEQPEPIEAIAEVPDGPPLR
                     FKWRRATHHVAAIEGPERISAEWWRAEGPTRDYFRAEDSEGRRFWLYREGLYGREVAR
                     PRWYMHGLFG"
     gene            10302..10655
                     /locus_tag="VKU03_01325"
     CDS             10302..10655
                     /locus_tag="VKU03_01325"
                     /inference="COORDINATES: protein motif:HMM:NF016370.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HLJ69925.1"
                     /translation="MRDDISLSAKKRARAMRRKPTEAERKLWYTLRDRRMQNVKFRRQ
                     APVGPYIADFLCVEHRLIVEADGGQHGESTRDSHRDAWLTKNGYKVLRFWNRDIFLNR
                     ESVVATIAAACGLPW"
BASE COUNT         1864 a         3266 c         3576 g         1989 t
ORIGIN      
        1 agtggatgac cgtggcggag aatatggctc tcgcccaagg cttcccccgc cgcgattggc
       61 tcggtcgttt cggtttcatc gattggcggc gggccgagaa acgcgcagac gaggcgctcg
      121 ccttgatcgg ctgccagatc gacccgagcc ggcgcatccg aagcctcagc cgtaccgaaa
      181 aatcgctggt ggcgatcgcg cgggccctga tggtcgaatg cgactttctc gtcctcgacg
      241 agccgacggc gagtcttccc gcggacgagg tcgagcgctt gttcgacgcc gtgcgccgcc
      301 ttcggcaaaa cggcgtcggg atgatctatg tgtcgcatcg gctcgatgag atttttcgga
      361 tcgccgatcg cgtcgtcgtg ttgcgcgatg gaagatttgt cggcggcaag ccggtgaagg
      421 agaccaatcc ggacgaattg atcggtatga tcgtcggccg ctcgacgctg gatttgttcc
      481 acaaggccga gatcgccgcc gggcccgacc gggtcgtggt acgaaatctg gtgacgccgc
      541 gcgcaggtcc ggtcgatttc gagattcgcg aaggcgaatt gctgggcctg gttggcctgc
      601 gtggcgcggg gcaagaggaa atcggccgcg cgctgttcgg cgccgctccc catagcggtt
      661 cgatcctcat caatggcgaa agtcccgaac tctcgtcgcc gcgcgccgcg ctccgcgcgg
      721 aaatcggtct ggtcgcgcgc gatcgcgccg aggaatccgt cgcgacctcg ctcagcgtcc
      781 gcgaaaacgc ctttctcaat cccggcgctt cgggccgcgg cctcttctca cggctgtcgc
      841 cggccgccga agcgcgcaag gccgtcgcgc tcggcgcgtc ggtgggcttg cggccgaacg
      901 atcccgacct gccgatcgaa ggtctatcgg gcggcaatca gcaaaaggtc attgtcggtc
      961 gctggctggc gacgggacga cggcttttga tcgccgagga tccaacggcc ggcgtcgacg
     1021 tcggcgccaa ggccgagatc taccggctga tcgaacgcgc gctgcaggaa aagctcgccg
     1081 tggcggtggt ttcgaccgat ttcgaagaga tcgcccatat ctgccatcgt gcgcttgtct
     1141 tcaatcgggg acggatcatc gcggaattgt cggggccggc gctgaccaca gaggcggtga
     1201 tcgccgccgc ctcggcgtct gaggcggctt aaggcgcagg ggcagggagg ctcgcatggc
     1261 gacgaattcg atccgctcag acgctttgga gccgacgcgc ggcgagttga agaatgtatc
     1321 tttcgcaatg cgggtgacgc gtctcctgcc ggtttatggc ctgccgatcc tgacaatcgc
     1381 gttgatcgcg ctgttctcga tcgtgttgcc cgacacgttt ccaaccatgc tcaatctgcg
     1441 ttcgatcatc gccgacaagg cgattatcgc gattctttcg ctcgcggcga tgatcaccat
     1501 ggcctcgggc aagatcgatc tcaccgtcgg ctatggcatc gtcatctggc atattctggc
     1561 gatcagcctg caatcggtct ttggcctgcc ctggccgctg gcggtgatca tcgtacttct
     1621 cctcggcgca ttcgtcgggt tgatcaacgg cctcctcgtc gaagtcgcgc agatcgattc
     1681 cttcatcgcg acgctgggcg cgggcaccgc gctttacgca attgcgcttt ggcataccgg
     1741 cgggcggcaa gtcgtcgcga atctgccgca tgggttcctc gcgctcaacg gaacgatgat
     1801 tttcggcctg ccgatcaccg gctactatgt gatcgggctc gccgtaatct tatggttcgt
     1861 tttcgagcat ttgccggtcg gccgctacct ctacgcgatc ggcgccaatc ccaaggcggc
     1921 ggagctgaac ggcataccgg tacgccgttt cacggtcgca gccttcgtcg cttccggcgt
     1981 gttgacggcg gtcgccggtg tgcttctggc gtcgaaactc cgtatcgggc aggcgagcgt
     2041 cggcttggaa taccttctgc cggctttggt cggcgccttt ctcggttcga cgacgatcaa
     2101 gcccggccgc gtcaatgtct ggggtacggt cgtcggcgtc gccattctcg ccgtcggcat
     2161 cgcgggcatt cagcaattcg gcggctcgtt ctgggtcgag ccgatgttca acagcgtcac
     2221 gctgcttgtc gccatcggca tcgccggcta cgcccagcga cgccgcggcg cggcggagcg
     2281 acagatcaaa aagacgtccg catgatgcgg ccgtcgcagc agggaggaaa cgcatgtcga
     2341 gattgttgtt gaggacggcg gtcagcggcc tggcgcttgc gctggcgatc ggcgcgtcgc
     2401 acgccgacgg cctcgtcgac gaggccaagg cgatcgtcgt caaagcgacg agccgcgccg
     2461 acaaatggga cggtccgact tccggcccga aagcagaggc taagaagacc gtggtctatg
     2521 tcgccggcga catgcgcaat ggcggcattc tcggcgtcgc ccatggcgtc aaggaagcgg
     2581 cggacacgat cggctggacg taccgcgaga tcgacgggca gggcacggtt tcgggtcagg
     2641 ccgccgcgct gggccaggcg atcgcgctga agcctgacgc gatcatcgtc ggcggcagcg
     2701 atgcggtaca gcagaaggcc ggcctcgaag acgccgccaa gcaggggatc acgatcgtcg
     2761 gctggcatgc cggaccgaag ccgggtccga tggacggcgc gccggtcttc gccaatgtca
     2821 cgaccgacgc gatggaagtt gcgaaagtcg cggcgatgaa ggcgattgcc gattccaacg
     2881 gaacggcggg cgtcgtcgtc ttcgccgatt cggcctatgc gatcgccatc gccaaggggc
     2941 gcgccatgga ggcggagatc aagaaatgca gcggctgcaa ggtcctcgcc ttcgaggata
     3001 cgccgctcgc cgacaccgcc ggccgcgtgc cgcagctcac cacgaccttg ctgcagcaat
     3061 tcggcgcgaa atggacccat tcgctggcga tcaacgatct ctactatgat tcgatgggcc
     3121 cctcgcttca ggtcgcggga ttgaagggcg acggcgatcc gcacaatatt tcggcgggcg
     3181 atggttcgga atccgcctat cagcgcatcc gcgacaagga ctatcagtcg gcgaccgtgc
     3241 ccgagccttt gaacatgcag ggctggcagc tcgtcgacga gatcaatcgc gcattcgcga
     3301 aggagaaatg gtcgggcttc gtgccgggcg tgcatctcgt cacggccgac aatatcgctt
     3361 tcgacggcgg tccgaagaat gtcttcgatc ctgacaatgg ctatcgcgac caatataaga
     3421 agatttgggg cgcgaagtag gtttgaccgg ccggcgttta tctgagacgc cggccgatga
     3481 aaatatgacc gatagtttcg aaaagtcagg gcgcaatcag cccactatgg cggatgtcgc
     3541 cgcgcgcgcg ggcgtgtcgg cgatgacggt gtcgcgcgcg ctcggcaagg gcggttcgat
     3601 cgcggaaagc acgcgccgcc gcatcatggc ggcggcggat gagcttggct atgtattcga
     3661 ccaggccgca gggacgcttt catcgaagcg ctcgggcttt atcgcggtgc tgatcccggc
     3721 tttcgacaat tccaacttcg ccgatacgac gcgcggcctc gccgaagccg tcgaatcctc
     3781 gggactgcag gttctattcg gctgtacgga ttattcgctc gataaagaag agcggctgat
     3841 cgaatccatg ctccggcgcc ggccggaagg gatgatcgtc accgacggtc gtcatacgga
     3901 gcgcgcgaat cgccttcttc gtacttccgg cgtcccggtc gtcgagactt gggacatgcc
     3961 gagcgacccg atcgagcatg tcgtcggctt ctccaacgcc gatgcgatga acgccgtggt
     4021 tcatagcctc tatcaacgcg gctaccggcg catcgctttt atcgggagcg ccagcgcgcg
     4081 cgatacgcgc ggcggcgatc gcgagcgcgg ctatcggcaa gcggtcggga gcttggatat
     4141 gcccaaggga cggatcgtct cgatcggcga gccgccgatg tcgatgacgc acggctgcgc
     4201 ggcgatcgtc gacgttctgt cgcgatggcc ggacaccgac gccgtcgcct gcctatccga
     4261 tctcgccgca ttcggcgctt tgatggaatg ccgaaggcgc ggatgggatg tgccgggacg
     4321 cgtcgctttg gcggggttcg gcgatttcga attatcgcgt accgccgcgc cgcggatcag
     4381 cacggtcttc gtcgacgctt tcgcgatcgg acgcgctgcg ggcgagatca tgctgcaagc
     4441 gatcgaggcg gcgcggagcg gcggcagtct cacgccacag atcgtcgccc agcccttccg
     4501 cgtgctcgaa cgcgagagcg cgtgaagccg tctagccgcc gggtttcgtc gcgacaatga
     4561 acaggcgcgg atagaggaaa agcacgcagc cgtccggctc gctgggatag gccgcggcga
     4621 gctcctccat atagcgcgcg atgaattgtt cgcgctcggc ctcgtcgagc ggattgagga
     4681 agggacgcaa gccgctgccg cggaaccaat cgacgagttc gccgacgccg ttcagcgcgt
     4741 gaatataggt cgtctgccag atttccaacc ttgcgccgag cggccgcagc cagcgataat
     4801 agtcggaata ggcgccgatg accgcgcgcg ttttggcgat cggcacgagg cgatcggtcc
     4861 aaggtccgtc cgcagcgacc atgcgcatca acgcatgcga cggctcctgg cgattgtccg
     4921 gcatctggat cgcgagcgag ccgccttcgg cgagcagaga atagagacgt ggtgtcaatt
     4981 catgatgatc gggcagccat tgcagcgagg aattcgcgaa aatgatgtcc ggcggcgtcg
     5041 tcgggcgcca ggcggcgata tcgcgctttt caaaattgat tttcggcaac cttgtacggg
     5101 cgctctccaa catgcgatcg gaactatcga gaccgatcac gtcggcttcg ccgaaccgcg
     5161 tttgcagcag acgggtgctg gcgcccggcc cgcagccaag gtcgacgacg cggcgtgggg
     5221 ttcgggttgg cacatgggcg aggagatcgt tcgcggcgcg tacccgctct ttctcgaatt
     5281 taagatagag agcgacgtcc cattcgttgg catgaatgaa aggcgaggcg cgttcagacg
     5341 tctcaatcgg ctgagtcact gggcccctcc cggacagaca ttaaggaggg agattggcga
     5401 gcaatatgtc caatttcagg cctgttttgg agcgtgtcca aagagttgaa tcgctgtgat
     5461 ccgcggaagg gcgcgcaatc agcaacggat aagccacgtt cttaatcatt cgcgtgaaag
     5521 cgctgcttgc gataatcttt cctttagtcg cggcgtcgcg aaatcgagaa aggcgcgcag
     5581 cttgagcggc gtcaggccgc caggtccgcg caccaggctg accggccaag gcggcggttc
     5641 gaaatcctcg agcgcaagaa ccagcgcgcc ggctctgacc gccgcgacgg cttggtacga
     5701 gaagacgcgc gcgaggccga ggccggcgat cgccgcgtcg atggccgcct cggccgtggt
     5761 gacagtaaga cgcgagtgaa ttggcgttgc gacattcttt ttgtccaaag cgaaggtcca
     5821 atgcgcgggc gagctcaagc cttcgaacgt gatgcagtcg tggccgtcga gatcggccgg
     5881 cgtctttggc gcgccgcggg cggcgaaata ggcggggctc gcgcaaacga cgcggcggat
     5941 cgcgccgact ttcgtcgcca gaaggctgct gtcctcgagc cgggcgatgc ggacagcgag
     6001 atcgatgcga tcttcgagga gattcatcac gcgatcggcg aggatcaggc gaacatcgat
     6061 tcgtggatag gctttgagaa attccgtgac gatcggcaat atatggagcc ggccgaagac
     6121 gatcggcgcg gtgagggtca gttcgccctt cgcctcgcta tattcgccgg cggcggcgcg
     6181 ttcggcggcc tcgacctctt cgagaatgcg cctgcaggcc gcgacatagg cttcgccggc
     6241 ttcagtcaag gaaaggcgcc gcgccgagcg gatgaggggc cgcgccttga gcgccgcctc
     6301 cagttccgag actttgcggc tgacggtggc gagcgggacg ccgagacggc gcgctgcggc
     6361 cgagaggctg ccggcttcgg cggcggtcac cagaatggac atggattcga ggcggtccat
     6421 cgctccctcc cggatattgg gaggaatgat atcagaaacg ccgtctaccg cggaattacg
     6481 gaaggattaa atcggtgtgg ctttggcggg cgcggctgcg cgcacgcctc gagaggctat
     6541 gatgagcgac ctcagacatt attcgagcga catcgccttt acgccgaggg tcaaggcgat
     6601 ccaggcgcag aaaggctcgc ggcaggccta tgccgaaatg gaggagcagg gcgcgtggga
     6661 gacccgcgtc acgccggatc tggcccaatt catcggcgcg caaaggagcg tctttctcgc
     6721 cacggccaat tccgaggggc agccctatat ccagcatcgc ggcgggccgc caggtttctt
     6781 gcgcgtgatc gatgagaaga cgatcggctt cgccgatttc gccggcaacc ggcagtatat
     6841 cacgctgggc aatctcgccg acaatccgaa ggcctacctc ttcctcatcg attatacgat
     6901 gcgccggcgc gtcaaaatct ggggcgaagc gcgcatcgtc gaggacgatc cggcgctgac
     6961 ggcgagcctg gcgacgaagg gctatcgcgc gcggccggag cgggcgctcc tgttcacgat
     7021 cggcgcctgg agcgtcaatt gcccgcagca tattccgcaa cgtttcgaag ccgccgacgt
     7081 cgccgccgcg ctggcggaac gcgatcagag gatcgcggat ttggaggcgg aagtgaaggc
     7141 tttgcgggag gcggtgaagg cggagtaaat ggctgtctga cgctagcgcg gctccggcgc
     7201 gccgcaatgg ggacaaaaag gtatcttctg ttttgggtgc atcacccact tgaaatgaca
     7261 gtttgggcat tccgcgtgaa tatgagcgat ggcgcggttt tgccaagttc gaaacgtgag
     7321 gcagaatagc aaaacagcga aatcctcggc tgtattttcg aggacgatgt acaatattct
     7381 ctcatgcccg tgatcgtgcc agtttgtggc gatatcatcg agtaaaaaga cgacgaaggg
     7441 gatgacaaag atgtaccatc catagcgtcg gacaaaagcc atcgtggcgg ctccctcgga
     7501 ccggctctcg catatctctg aaatcgttaa ttggagttgg taaaccgata gaacccaata
     7561 agagtacaaa gccgactccg gcgctgtaag ttcgagatac gtttaatcgg gcgtgaatta
     7621 actctcatta tcgatgcgtc ttcccgtttc ctcttgactc tccgggcaat cgattattgt
     7681 atagaacaaa gagagaacat gccggaatat tcatatcatg tcgtcgggtc aaagagtgca
     7741 ggccgtggcg catttgcgca gcgccattgc ggggatcgaa tcccaggaag ggccgcgccc
     7801 gcgcgcgcct ttccggctgc cggtcgcccg ggcgctggac ggggcgctgg gcggcgggct
     7861 tgccggcgat gcgcttcatg agatcgcgcc ggctgctccc ggcgacggcg cggcggccat
     7921 ggctttttcg ctctgcctgg cggcgcggtt tgtggccgct tcgccgcgcc gtttctgcct
     7981 gatcgcttac gaggaattct gcgcccgcga ggctggcgcg ctttatggac cgggcctcgc
     8041 ggcctttggc ctgcggcgcg acgagcttgt tttcgtcaag gcgcaggatg ggcgcgcctt
     8101 gctctggacg atggaggagg cgctcaaaag cggcgcgctt gccgtcgtcg tcggcgagct
     8161 ttggagcgtc gccaaacatt acggcctcgc cgcttctcgc cggctgcttc tggcggcgcg
     8221 agcaggcgcg acgccggcgc tgatgatcca tggcggcgtt ttcggtcagg ccaatgcgct
     8281 ctccagcgcc gccgagacgc gttttgaaat cgccgccgcg gcgagccggc gcttgccgcc
     8341 ggccggcggc cgcctcgccc tgccgggcgc gccggccttc gccgcgcggc tcgtcaagtc
     8401 gcggctttcg ccctccgctc aagggccgcc ggcgcggctt ttcgacgaga cgcgcgttga
     8461 gcgtctcgaa tggtcgagcg ctgagaaaag cttccatgat ccgacggtac ctctccctct
     8521 ggttcgcgcg cctctcgacg gaccgcgcgc gcgtgttgcg ggaaaatgag cgcagcgctg
     8581 cgcgcgccct cacgccgctg gcgacgtacc tcaagatcaa aaacgcgcag caactcgtct
     8641 gcatcgatcg gcaggcggcg agtcttggct tgacgcgcgg cctgccgctg gccgatgcgc
     8701 gggcgcgcca tcccaatctt gtcgcggtcg aagccgatcc ggcggaagag ggaaagctcc
     8761 tcgcccggat cgccgattgg ctgcagcgtt tcacgccgct cgtcgcgctc gacggcgccg
     8821 acgggatcat gctcgacatc gccggcgtcg cccatctctt cggcggcgaa aaggcgatga
     8881 tccaggagat cgagacgcgc ctggccgcgc agggccttgg cgcgcgggct ggaatcgccg
     8941 attttccgcg cgcggcctgg gcgctcgcgc gtttctccaa ggagcgtatt gcgccgaccg
     9001 gcgcggagag aaaaatcgtc gcgcaattgt accatggcct gccgctcgcc gcgcttgggc
     9061 tcgctgagac gaatgtcgcc gacatggccc gcgccggtct tcgccgcatc ggcgatgtcg
     9121 ccttgcgtcc gcgcgcgccg atcgctgcgc gtttcggcgc cgacgtcatc gcccggctcg
     9181 acgcgcttta cggcctggag cgctcggcga tttcgccccg ttttgccgcg cctgaatttt
     9241 ccgccgagcg gcgtttcgcc agcgcgatcg ttcagaaaga ggcggtcgag gcgacgcttc
     9301 tgaaactcgc ggaggatttg gcggcgttgc tgcagcgtca ggtcaaaggc gcgcggcggc
     9361 ttgaattagc gctctttcgc gtcgacggcg ccgtgcgccg catcgtcgtt ggcgccggcc
     9421 ggccgctcaa tgagccgcgc gcgatggcgc gtctgttccg cgagcgtttc gaggcgcccg
     9481 acgaggacga gatcgacgcc ggctatggtt tcgatgtggt gcggctttcg tgtcttctcg
     9541 ccgagccgct tagccccgcg caaggcgatt tcgcccgcgc gccggaagaa aaagagacgc
     9601 gttcgctcgc cgatctgctc gaccgcttga gcgcgcggct cgggatgcgc cgcgtgaccc
     9661 ggatcgatct cgtcgagacg catattccgg aattcgccgt tgtggcgagg ccggcgactt
     9721 tgggcgacgc gcgggccccc ttcaccatgc ttcgcatggt ccccctcccc cgctgcgcgg
     9781 gggaggagaa aggcggcgcg aagcaagggg aaggggctcc gccgacgcgt cccctccgtc
     9841 tcctcgaaca gccggagcct atcgaagcca tcgccgaagt ccccgacgga ccgccgttgc
     9901 gcttcaaatg gcggcgcgca acgcatcacg tcgcggcgat cgaaggtcct gaacggattt
     9961 ccgctgaatg gtggcgcgcg gagggaccga cgcgcgacta ttttcgcgcc gaggattccg
    10021 aaggccgccg cttctggctc taccgcgaag gcctctacgg ccgcgaagtc gcccggccgc
    10081 gatggtacat gcatgggttg ttcgggtgag gcggcggctt accctcatcc ggcgcttcgc
    10141 gccaccttct cccgcaggcg ggagaagggg tggttggcgc gcttattctg gcgagggcgc
    10201 ggatgacggc ctcggcgcgc cccctctccc gcttgcggga gagggttggg gtgaggggaa
    10261 gcctcgaact tcgacacagc actcgtacat gaggaccatc gatgcgcgat gacatctcct
    10321 tgtccgcaaa gaagcgcgct cgggcgatgc ggcgcaaacc aaccgaagcg gagcgtaagc
    10381 tctggtacac attgcgcgac cgacgtatgc aaaatgtcaa gtttcgaaga caagcgccag
    10441 tcggcccgta cattgcggat tttctttgcg ttgagcatcg gttgattgtc gaagcagacg
    10501 gcggacaaca tggagagagc acaagagaca gccatcgtga cgcctggttg acaaaaaatg
    10561 gatataaagt tctgcgcttt tggaaccggg acatcttcct caaccgcgaa agcgttgtag
    10621 cgacaatcgc cgccgcgtgc gggttgccat ggtgaggcgg cggcttaccc tcatccggcg
    10681 cttcgcgcca ccttc
//
LOCUS       DATGJW010000046         3192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_527182, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000046 DATGJW010000000
VERSION     DATGJW010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3192
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527182"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VKU03_01330"
     CDS             complement(<1..309)
                     /locus_tag="VKU03_01330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HLJ69926.1"
                     /translation="MKETGTFYRRDRGWHPPPLTPQYKTSVLRSPRVALLSLENTISE
                     LTGPVFGHDILGARDNDLIVNYAKTGEAIGPRINVYGRVLDENGRGVDGVLVEIWQAN
                     A"
     gene            complement(454..879)
                     /gene="pcaC"
                     /locus_tag="VKU03_01335"
     CDS             complement(454..879)
                     /gene="pcaC"
                     /locus_tag="VKU03_01335"
                     /EC_number="4.1.1.44"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047575 - 4-carboxymuconolactone
                     decarboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxymuconolactone decarboxylase"
                     /protein_id="HLJ69927.1"
                     /translation="MNEEKKSERFAAGMKTRRAVLGDAHVDRASAAMTDFDEPFQTFI
                     TEGAWGAVWSRPQLAKRERSLITIALLAALGHDEEVAMHVRATANTGASPEDVREALL
                     HVAVYAGVPAANRAFKIVKRTYAEMASILPREAGEGDRA"
     gene            complement(876..1658)
                     /gene="pcaD"
                     /locus_tag="VKU03_01340"
     CDS             complement(876..1658)
                     /gene="pcaD"
                     /locus_tag="VKU03_01340"
                     /EC_number="3.1.1.24"
                     /inference="COORDINATES: protein motif:HMM:TIGR02427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047570 - 3-oxoadipate enol-lactonase
                     activity [Evidence IEA];
                     GO_process: GO:0042952 - beta-ketoadipate pathway
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipate enol-lactonase"
                     /protein_id="HLJ69928.1"
                     /translation="MRTNSGVAHYADEGPRSARAIVFVNSLGTDLRIWDEVAAPLAKQ
                     WRAIRYDKRGHGLSSHPVAAASIGDFAADLAGLIDNLQAGPATIVGISIGGLIAQELY
                     RRRRDLVASLVLCDTGHKVGTPEFYNSRADVVQSGGIESIAEGVLERWFTADYRTNNP
                     DMMAGWRAMLTRTPKDGYVAACRTVGQPDFTEAAKAVAAPTLVIVGDEDAATPPELAR
                     QLAGLIPRARLEIVAGAGHLPCIEKPDVMRALIEAHVKAISP"
     gene            complement(1759..>3192)
                     /locus_tag="VKU03_01345"
     CDS             complement(1759..>3192)
                     /locus_tag="VKU03_01345"
                     /inference="COORDINATES: protein motif:HMM:NF026047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLJ69929.1"
                     /translation="RPNLGIVLDSFHIGVRRNPIEPIAALPAERIALVQIADAPAIEM
                     DPLSLSRHYRCHPGQGDYPLIDFLDAVTRAGYRGPVSLEIFNDQFRGASAATIALDGL
                     RALKMSGEALAKKRQARGDPPLADLAPLPEAPRVEAVEFIEFAVAERESAALAALLGG
                     LGLVRAARHRSKDVDLYRAGAINIVVNRERESFAHSFNLLHGPSICALALRVDKVEQA
                     LERAGAMECPTYFGRIGPGEASIPAVAGIEGSLVYFVDADSHWERDFVADADVEAQAP
                     LRKIDHLSNVVRRSEFLTWALFYKSVLGFHSEPQVEIADPHGAFFSRSLRSPNDEARI
                     ALNVSDGGATIATRFLDAFGGAGFQQIALLAEDIFAVVEAARAKGVAFLDIPDNYYDD
                     LAARFDLAPDLLAGMRNLGVLYDRVKDGEFFHIYTRTFQDRFFFEIVQRKNYDLFGAA
                     NTPVRLAAQARAQDEEAALRLDLESAP"
BASE COUNT          543 a         1026 c         1084 g          539 t
ORIGIN      
        1 ggcattggcc tgccagatct cgaccagcac gccgtccacg ccgcggccgt tctcgtcgag
       61 aacccggcca tagacattga tccgcggacc gatcgcttcg ccagtcttgg cgtagttgac
      121 gatcaggtcg ttgtcgcgcg cgccgagtat gtcgtggcca aagaccggtc cggtcagttc
      181 ggaaatcgtg ttctcgagcg aaagcagcgc gacgcgcggc gagcgcagca cggaagtttt
      241 gtattgcggc gtcagcggcg gcggatgcca gccgcggtcg cggcgataga atgtgccggt
      301 ttctttcatt cccaagcctt tcggttcttt cctcccccgc gcagcggggg agggggacca
      361 tgcgaagcat ggtggagggg gccgcggccg cgagggataa tcaccagctc cggagccccc
      421 tccaccgcgc tacgcgcagc cccctccacc gcgctacgcg cggtccccct cccccgcttc
      481 gcgggggagg atagaagcca tctcggcgta tgttctcttc acgatcttga aggcgcggtt
      541 ggcggcgggg acgccggcat agacggcgac atgcagcagc gcctcgcgca catcctcggg
      601 gctcgcgccg gtattcgccg tggcgcggac atgcatggcg acttcctcgt catggcccag
      661 agcggcgagc aaggcgatgg tgatcagcga gcgctcgcgc ttggcgagct gcggccgcga
      721 ccagaccgcg ccccaggccc cctcggtgat gaacgtctgg aacggttcgt cgaaatcggt
      781 catcgccgcg ctggcgcgat cgacatgggc gtcgcccagg acggcgcgcc gcgtcttcat
      841 accggcggcg aaacgctcgc tctttttctc ttcgttcatg gcgagatagc cttcacatgc
      901 gcttcgatga gcgcgcgcat aacgtccggc ttttcgatgc agggcagatg gccggcgccg
      961 gcgacgattt cgagcctggc gcgcgggatg aggccggcga gctggcgcgc cagctcgggc
     1021 ggcgtcgcgg cgtcctcgtc gccgacgatc accagggtcg gcgccgcgac cgccttggcg
     1081 gcctcggtga aatcgggctg gccgaccgtc cggcaggcgg cgacgtaccc gtccttcggc
     1141 gtccttgtca gcatggcccg ccagccggcc atcatgtcgg gattgttcgt ccggtagtcc
     1201 gccgtgaacc agcgctccag cacgccctcg gcgatggatt cgatgccgcc gctttgcacg
     1261 acgtcggcgc gggaattgta gaattccggc gtaccgacct tgtggccggt gtcgcagaga
     1321 accagcgacg cgacgaggtc gcgccggcga cgatagagct cctgcgcgat caggccgccg
     1381 atcgaaatcc cgacgatcgt cgccgggccg gcctgcagat tgtcgatgag gccggcgaga
     1441 tcggcggcga aatcgccgat gctcgccgcc gcaaccggat gcgaggagag gccgtggccg
     1501 cgcttgtcgt aacggatcgc gcgccattgc ttagccaacg gcgccgcgac ctcgtcccag
     1561 atgcgcaaat ccgtgccgag cgaattgacg aagacgatgg cgcgcgccga tcgcggcccc
     1621 tcgtcggcgt aatgcgcgac cccgctattg gtccgcatga atttcacggt tcgccgctcc
     1681 tccctgccac ggaatcgttt gtcacccaac aattatattc tataagattt aacgcaagct
     1741 tgaaaccgcc gcgtcgcttc agggcgccga ttcgaggtcg agccggaggg cggcctcctc
     1801 gtcctgcgcc cgcgcttggg cggccaggcg caccggcgtg ttggcggcgc cgaacagatc
     1861 ataattcttt cgttggacga tttcgaagaa gaatcgatcc tggaacgtgc gcgtatagat
     1921 gtggaaaaat tcgccgtcct tgacccggtc gtagagcacg ccgaggttgc gcatcccggc
     1981 gaggagatcg ggggcgaggt cgaagcgcgc cgcgaggtcg tcgtagtaat tgtcgggaat
     2041 atcgaggaag gcgacgcctt tcgcccgcgc cgcttcgacg acggcgaaaa tatcctcggc
     2101 gagcagcgcg atctgctgaa agccggcgcc gccaaacgcg tcgagaaagc gcgtcgcgat
     2161 cgtcgcgccg ccatccgaca cgttgagcgc gatgcgcgcc tcgtcattcg ggctgcgcaa
     2221 cgagcgcgag aagaaggccc catgcgggtc ggcgatctcg acctgcggct cgctgtgaaa
     2281 gccgagcacc gatttataga acaaggccca ggtcaggaat tcgctgcggc ggacgacatt
     2341 cgacagatgg tcgattttgc gtaaaggcgc ttgcgcttcg acatccgcgt cggcgacgaa
     2401 gtcgcgctcc caatgcgaat cggcgtcgac gaaatagaca aggctgccct cgattccggc
     2461 gaccgccgga atcgaggctt cgcccgggcc gatcctgccg aaataggttg gacactccat
     2521 cgcgccggcg cgctccagcg cttgctcgac cttgtcgacg cgcagagcga gcgcgcagat
     2581 cgatgggcca tgcagcaggt tgaaggaatg ggcgaagctt tcgcgctcgc gattcaccac
     2641 gatgttgatc gcgccggcgc gatagaggtc gacgtccttc gagcggtggc gcgcggcgcg
     2701 gacgaggccg agccctccga gcaaggcggc aagcgcggcg ctttcgcgct cggcgacggc
     2761 gaattcgatg aactcgaccg cctcgacgcg tggcgcttca ggcagcggcg caagatcggc
     2821 gagcggcgga tcgccgcgcg cctgacgctt cttggcgagc gcttcgccgc tcatttttaa
     2881 ggcgcgcaag ccgtcgagcg cgatcgtcgc cgcggaggcg ccgcggaatt ggtcgttgaa
     2941 aatctccagc gaaacggggc cgcgatagcc ggcgcgggtg acggcgtcga gaaagtcgat
     3001 cagcgggtaa tcgccctggc cgggatggca gcgatagtgg cggctcagcg acagcgggtc
     3061 catttcgatc gccggcgcgt cggcgatctg cacgagcgcg atgcgctcgg ctggcaaagc
     3121 ggcgatcggc tcgatcggat tgcgccgcac gccaatatga aaactgtcga gcacgattcc
     3181 gagattgggc ct
//
LOCUS       DATGJW010000047         2474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_112181, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000047 DATGJW010000000
VERSION     DATGJW010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2474
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112181"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VKU03_01350"
     CDS             <1..536
                     /locus_tag="VKU03_01350"
                     /inference="COORDINATES: protein motif:HMM:NF013899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="urease accessory protein UreD"
                     /protein_id="HLJ69930.1"
                     /translation="ATRLSLAAGARLAWTPQETILFDKARLERRLDADIAEDAALLLI
                     ESVVFGRLAMGEDGIAASLSDRWRIRRGGRLVFAEELRIGDAAAALSRPAVGGGARAI
                     ASFLFMAPNAAAVLDKVRAALGEATGDGAAAEAGASALDGFIVGRAVAAEPVRLRALV
                     LAVMMALTGRPAPRVWQ"
     gene            668..1288
                     /locus_tag="VKU03_01355"
     CDS             668..1288
                     /locus_tag="VKU03_01355"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009039 - urease activity [Evidence IEA];
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0043419 - urea catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit gamma"
                     /protein_id="HLJ69931.1"
                     /translation="MNLTPREKDKLLIAMAAIVARRRLDRGVKLNHPEAVALIADFVV
                     EGARDGRSVAELMEAGAHVLTADQVMPGIAEMIHDIQVEATFPDGTKLVTVHDPIRGA
                     DDRLKPGEVLAEPGDILMNQGRETLTITVSNSGDRPIQVGSHYHFFETNPALKFDRAK
                     TRGFRLDIPAGTAIRFEPGQSREVRLVALAGKREVYGLRQEVMGKL"
     gene            1504..>2474
                     /locus_tag="VKU03_01360"
     CDS             1504..>2474
                     /locus_tag="VKU03_01360"
                     /EC_number="3.5.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010682666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease subunit alpha"
                     /protein_id="HLJ69932.1"
                     /translation="MAKAKSHNEPAHASLPRAAYADMFGPTKGDRIRLADTDLIVEVE
                     KDFTTYGEEVKFGGGKVIRDGMGQSQVARRRGAVDTIITNAVILDHWGIVKADVAIKD
                     GLIAGIGKAGNPDIQPDVNIVIGPGTEAIAGEGKILTAGGFDTHIHFICPQQIEEALM
                     SGVTSMLGGGTGPATGTFATTCTPGPWHIARMIEAADDFPMNLGFAGKGNASKPAALI
                     EQIEAGACALKLHEDWGTTPAAIDCCLSVADDHDIQVMIHTDTLNESGFVEETIKAFK
                     GRTIHAFHTEGAGGGHAPDIIKVAGLNNVLPSSTNPTRPYTKNTIDE"
BASE COUNT          441 a          822 c          844 g          367 t
ORIGIN      
        1 tcgcaacgcg cctgagcctg gcggccgggg cgcggctcgc ctggacgccg caggagacga
       61 tcctgttcga caaggcgcgg ctggagcgcc ggctggacgc cgacatcgcc gaagacgcgg
      121 cgctgctgct gatcgaatcc gtcgtgttcg gccgcctcgc catgggcgag gacgggatcg
      181 cggcgagcct gagcgatcgc tggcgcatcc gccgcggcgg gcgcctcgtc ttcgccgagg
      241 agctccgcat cggcgacgcc gccgcggcgc tcagccggcc ggcggtcggg ggcggggcgc
      301 gggcgatcgc ctcttttttg ttcatggcgc ccaacgcggc ggcggttctc gacaaggttc
      361 gcgcggctct cggcgaagcg accggggacg gcgcggcggc cgaagccggc gccagcgcgc
      421 tggacggctt catcgtcggc cgtgcggtcg ccgccgagcc ggtccgattg cgcgccttgg
      481 ttctcgccgt catgatggcg ctgacgggga gaccggcgcc gcgcgtctgg caatgagcgg
      541 cgccgcgctt gcacgctccg tcgtggcgag cgaagcaatc cattcacggg cagagcctta
      601 cgagtggatt cgccgctgcg ctcctcgcaa tgacaggtaa cgatttaacg tcttcaagag
      661 cacgccgatg aacctgacgc cgcgcgaaaa agacaagctg ctcatcgcca tggccgcgat
      721 cgtggcgcgg cggcggctgg atcggggcgt caagctcaac catcccgagg cggtggcgct
      781 gatcgccgac ttcgtcgtcg aaggcgcgcg cgacggccgc tcggtcgccg agttgatgga
      841 ggccggcgcg catgtcttga ccgccgacca ggtcatgccc ggaatcgccg agatgatcca
      901 cgacatccag gtcgaggcca cctttccgga cgggacgaaa ctggtcaccg tgcatgatcc
      961 gatccgcggc gcggacgata ggctgaaacc cggcgaagtc ctcgccgagc ccggcgacat
     1021 tctgatgaat cagggccgcg agacgctgac gatcaccgtg tcgaacagcg gcgaccggcc
     1081 gatccaggtc ggctcgcatt atcatttctt cgaaaccaat ccggcgctga aattcgaccg
     1141 cgccaagacg cgcggcttcc gcctcgacat tcccgccggc acggcgatcc gcttcgagcc
     1201 gggccaaagc cgcgaggtcc gcctcgtcgc gctcgccgga aagcgcgagg tgtatgggct
     1261 gcgccaagag gtgatgggga agctgtgaga cgaacgtgca gcgccgcggc ttaccctcat
     1321 ccggcgcttc gcgccacctt ctcccgcaag cgggagaagg ggttgtagcc gcggtgggcg
     1381 accgcggcta cagcgcggcg gctgatgaag gccgcacagg gccccctctc ccgcttgcgg
     1441 gagagggctg gggtgagggt aagccgccgc ctgatacaag aatgtacttc attggggcgc
     1501 agcatggcga aagcgaaatc gcataacgaa ccggcgcacg cgtctctgcc gcgcgcggct
     1561 tacgccgaca tgttcggccc gaccaagggc gaccgcattc gtctcgccga caccgacctg
     1621 atcgtcgagg tcgagaagga ttttacgacg tacggcgaag aagtgaaatt cggcggcggc
     1681 aaggtgatcc gcgacggcat gggccagagc caggtcgcgc gccggcgcgg cgcggtcgac
     1741 acgatcatca ccaacgccgt catcctcgac cactggggaa tcgtcaaagc cgacgtcgcg
     1801 atcaaggacg ggctaatcgc cggcatcggc aaggccggca atcctgatat tcagccggac
     1861 gtcaacattg tcatcggccc aggcacggag gcgatcgccg gcgagggcaa aatcctcacc
     1921 gccggcggct tcgacaccca tatccatttc atctgcccgc agcagatcga ggaggcgctg
     1981 atgagcggcg tcacttcgat gctgggcggc ggcaccggcc cggcgaccgg cacgttcgcg
     2041 acgacctgca cgccggggcc gtggcacatc gcccggatga tcgaggcggc cgacgatttc
     2101 ccgatgaatc tcggcttcgc cggcaagggc aacgcctcca agccggcggc gcttatcgag
     2161 cagatcgagg ccggcgcctg cgcgctgaag ctgcacgagg attggggcac gacgcccgcg
     2221 gcgatcgatt gctgcctgtc ggtcgccgac gatcacgata tccaggtgat gatccatacc
     2281 gatacgctga acgaatcggg cttcgtcgaa gagacgatca aggcgttcaa gggccgcacg
     2341 atccacgcct ttcataccga gggcgccggc ggcggccacg cgccggacat catcaaggtc
     2401 gccggactaa ataacgtgct gccgtcgtcg accaatccca cgcggcccta taccaagaat
     2461 accatcgacg aaca
//
LOCUS       DATGJW010000048        10189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_527474, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000048 DATGJW010000000
VERSION     DATGJW010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10189
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_527474"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1844)
                     /locus_tag="VKU03_01365"
     CDS             complement(<1..1844)
                     /locus_tag="VKU03_01365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HLJ69933.1"
                     /translation="MEDRTVIQPAAPAGSVRRGARLNGIYEIGPLIAHGGMGEVYRGF
                     NIVTNDPVAIKMILPELSGDPDAFAMFKREASVLLNLHHEAIVRYFVFSVDPELQRAY
                     LAMEFVDGPSLGKRLAQGPLPIDEVRILQRRLASALDAAHRLGVVHRDISSDNVILPG
                     GDPRRAKIIDFGIARSLRLGEGTIIGSRFAGKFNYASPEQLGLAGGEVGPKSDIYSLG
                     LVLAEAMLGRPVDMGGSQAETIEKRRKVPDLTAIDPSMRPLIAAMLQPLPKDRPESMA
                     AVAASLPASAPRARGDRGRRESSPVGRLAAAAAALIVIVSVGGTLYAYRDLLPWRETP
                     SLAPPVAEQSAAAPTSRPAPSEQASADANSSTSPNLPPLPTPSAASPGPAIPPPAAPN
                     EAVSAAPAPSPPAESVPSAKPAAHVPTADELVNALKSHKQAEAATSVAALPPPAGEQA
                     KANASPPPPAPAKANLPAPGAPQPKTNLPPPLPEPKAEAAPAPLPAPKVDAAPPLPEL
                     KAHPAPPLPAPKADASPPPPKTAAATPTPAATLRAPQDQLVLDQATVGKEYLADLPPF
                     SDSADPRGVALRAEPSLPEGLVLADLGHGFGRISGEPQKPGQYSFSIVA"
     gene            2157..2687
                     /locus_tag="VKU03_01370"
     CDS             2157..2687
                     /locus_tag="VKU03_01370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLJ69934.1"
                     /translation="MRASIVAALGLAAGLTVHPAFAGPAYKASTVADFFAKAALGKSR
                     AICFGTAADCPQPPPDGSAKFDLLVTFEFDSDRLTAAAKENLDQFAEALHDPRLKGQK
                     FEIDGHTDATGTEQYNLGLSERRAASVIAYLATKGVDARELIGKGFGKTRPRVADPFS
                     PENRRVETHLLEQPGG"
     gene            2893..3900
                     /locus_tag="VKU03_01375"
     CDS             2893..3900
                     /locus_tag="VKU03_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69935.1"
                     /translation="MPDPKQQIDAALGDGYYDEWKQMDVDLGSENNRFDSIRRLLNLP
                     FSPIFDRAYDDFRTVNDIARDYQQGMSLILAGRAVNVGKLKEIHDLYTLALGVAENDN
                     DLLEAFWGSGFAAAWVIFPFTLLQAKSNAMLAALKELKKELAEAEHEVNKAVWKRRIH
                     LVVSFVEAMFPEISLTGRAALYLGDVILDKALGPKNTSTAQKYFGDVNPGVKQFSEAV
                     HHIEAYGETAKGVFEWTGKASTVATFYVDFEEIGEGEERVEKLKSLMEKVKDAYDALH
                     KTIEDNKSKLMAFLAAFDRVVLAIESVRINGDNIRQALVDDAAKYRYPRGAAASWLAV
                     P"
     gene            4022..5755
                     /locus_tag="VKU03_01380"
     CDS             4022..5755
                     /locus_tag="VKU03_01380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLJ69936.1"
                     /translation="MTRQKGRFFLIAALAVAVACAALIGFRFIKRSSAPPPPASVATP
                     AANPVKPAGPISTIIDLPGDPVLVRRGPSAALRSVHIDAPIALTPNAAKFDGDAYFVA
                     SPLLSTNGGFMGKFPETGEGADQAAMIAVADQVDQSDAAMEAGFDDDGGANTPAYADA
                     LLPNAANVSQIEVKAGGANGQPQIKETVIKTVASEKLSDLLIRAGYSEESARAIEAAS
                     KALYNIQALPAGSVAIAEGALDVSGAYRVVQLALYEGGEYVGAVALAEAGGYGEGAKP
                     PVPEGVFNEGQSAPAAPAQYNLADGLYSAGVRNNVPEPVIREAIQLLGKMTDLKALAS
                     PEATMRLMFSRVFRGKSKSTGRIIYVGLVGPGGAVDCYPFAGADGEFRCFNPKGGAPA
                     APIPTPRPGAPPVGAGEIFPPIKGAPITSLFGMRFHPILHIMRLHAGIDFGAPIGSIV
                     RAVADGKVEIAGPVSGFGNHVRLKHNGFETSYSHLSEIPANITPGATVVAGQEIGLSG
                     NTGLSTGPHLHFEYYLNGVAVDPLPHMTGEAGAAIASAVSAPSPPSEQEIAGFNTAKA
                     TIDAALDAASR"
     gene            5851..7494
                     /locus_tag="VKU03_01385"
     CDS             5851..7494
                     /locus_tag="VKU03_01385"
                     /inference="COORDINATES: protein motif:HMM:NF012234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ69937.1"
                     /translation="MWFKIEGKRSSLGQKFVVRCKSELAGKAVFEERIVKGAPEARKT
                     AEILSTSLNVTATVYGADAAGEFVYGVYEMAEEASEASNPIIKRLFAESAAASLSFKD
                     RLLADIPEETIRGFATMAAALGYFAAQHKQIRRSSSPQISYSTLFRIFCGGGESSTLT
                     LASDDATIAILTQKLADLPGWMWLSELPGLGSADHGIVSPYARPNKVTLNIDGELQVL
                     RPRTVFRGVAPGSGAHFMLIAQLDDRHGRPRHSIGLVYREADEASIVALVRRLLQESN
                     PFKNRVVLINRDLRTVRSRMSERTWRDIIPHEQARAELNFITASIRNRDMLKAEGLSV
                     RRGLLLSGPPGDGKSSAIECFVNDIANEATIIVVEAVDHIRSLYHLAQMLAPTVVILE
                     DLDLITKARQNPYAIGGKDDVTGELLQVLSGGSAYADIVTIATTNHPEAIDDALSKRA
                     GRFDAHIRMGYPSEAEKQRILALYLDRYGVDDELTRRRLQQMLTRDLGRLHLVPAHIE
                     EFVKAGVKRARLARRAPEYLDFEPGIEATKSIAASKPAA"
     gene            complement(7600..8895)
                     /locus_tag="VKU03_01390"
     CDS             complement(7600..8895)
                     /locus_tag="VKU03_01390"
                     /inference="COORDINATES: protein motif:HMM:NF018516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ImpA family N-terminal
                     domain-containing protein"
                     /protein_id="HLJ69938.1"
                     /translation="MSSAMAATLDKLPTLAPISAEAPCGPDLDAAGDGEFMNFLAAAE
                     GRLPSAYFAFDRKTVDFAETLASGRKLLERTLDLRLIVLLAKMAILDRDLRGFAFWLS
                     ETARLIAEYWDEVHPRGEDGDFAVRMAQLATLNDGPVVILPLQYAPLAETQRDGAVNF
                     RAHLIALGEAALREGETAPDSATIEKILLNCDLDGLKQALAALQAIKAAVAQIRSVSI
                     EKGSFEQAPTFEALEPLAERMAAFVQAAVARRDPSIAPPEAPAPQAGQAESGAGAAAA
                     PSDFASLAEVDAALAGALGYFARAEPSSAAVLLIGQARQLLGKNIYEVMKVLAPNYAD
                     QARVFVGAEPAFSVPVSSVGPAAEPVDPAPAPTEPAASRAAAIALIDGVAAHFRKVEP
                     SSPVPFLLDRAKSLASRDFLGLLKELLSEEAIEQMKRGG"
     gene            9115..9651
                     /locus_tag="VKU03_01395"
     CDS             9115..9651
                     /locus_tag="VKU03_01395"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69939.1"
                     /translation="MATDYDYELNPPRLARQKDLDTCWACCMSGLLAANMSRRQASED
                     ELVRTYATTPTGGIDVSKLRVVAKDFSYLCNDFQTAADARSILSDRFVVDRLRGNGML
                     MAAWRVHDPNKPNEVFFHAQIVWGVIYLSNQDVGTERALMRTMNPFTAKYELYPLFTV
                     YRSDNMPLFTCWPSTSAP"
     gene            9759..>10189
                     /locus_tag="VKU03_01400"
     CDS             9759..>10189
                     /locus_tag="VKU03_01400"
                     /inference="COORDINATES: protein motif:HMM:NF017739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="contractile injection system protein, VgrG/Pvc8
                     family"
                     /protein_id="HLJ69940.1"
                     /translation="MSLSQDDRIGKLTTPLGENVLVLSRLDGGEHLSEPFEFRIEAVS
                     EQQNVDFSGALGKGVTVKLKTSDHKDRYFNGVLAEARWAGPRADLFVYQLVLRPWLWL
                     LSLTSDCRIFAQKSPKEIIKQVFSDRGFSDFRDELKSSYPTL"
BASE COUNT         1795 a         3351 c         3334 g         1709 t
ORIGIN      
        1 gcgacaatgc tgaaagaata ttgccccggc ttttgcggct cgcccgatat ccggccgaag
       61 ccgtggccga gatcggccag gaccaggcct tccggcaggc tcggctcggc gcgaagcgcc
      121 acccctctcg gatcggcgga atcgctgaat ggcggcaggt cggcgaggta ttccttgccg
      181 accgtcgcct ggtcgaggac aagctgatcc tgcggcgcgc gcagcgtcgc ggcgggcgtc
      241 ggcgtcgccg ccgcggtctt gggcggcggc ggcgaagcgt cggccttcgg cgctggaagc
      301 ggcggcgccg gatgagcctt cagctcggga agcggcggcg cggcgtcgac cttgggcgcc
      361 ggaagaggcg ctggcgcagc ctcagccttc ggctcaggaa ggggcggcgg caggttggtc
      421 ttcggctggg gcgcgcccgg cgccgggaga ttggctttgg ccggggcggg aggcggcggg
      481 gacgcattcg ccttggcctg ctcgccggcg ggcggaggaa gggcggcgac gctagtcgcc
      541 gcctcggcct gcttgtgcga cttgagcgcg ttgacgagtt cgtcggctgt cgggacatgc
      601 gccgccggct tggcggaggg gacggactcc gccggcggcg aaggggccgg cgcggcgctt
      661 accgcctcgt tgggcgccgc tggcggcggg atggccgggc cagggctcgc cgccgaaggc
      721 gtcggcagcg gcggcaggtt gggcgaggtc gacgaattgg cgtcggcgct cgcctgctca
      781 cttggcgctg gtcggctggt tggggcggcg gcgctttgct ccgccaccgg cggcgcgagc
      841 gacggcgttt ctcgccacgg caggagatcg cgataggcgt agagagtccc gccgacgctg
      901 acgatgacga tcaaggccgc ggcggcagcg gcgagacggc cgaccggcga actctcgcgc
      961 cgccctctat cgccgcgcgc ccgcggcgcg gaagcgggca acgatgcggc gacggcggcc
     1021 atgctttccg gccggtcctt gggcaaaggc tgcagcatcg ccgcgatcag cggccgcatg
     1081 gacggatcga tcgccgtaag gtcgggaacc ttgcggcgtt tctcgattgt ctcggcctgc
     1141 gagccgccca tatcgaccgg ccggccgagc atcgcctcgg ccaggacgag gccgaggcta
     1201 taaatgtcgg atttggggcc gacctcgccg ccggcgaggc cgagctgctc cggcgaggcg
     1261 tagttgaact ttccggcgaa acgcgagccg atgatcgtgc cctcgccgag ccgcagcgag
     1321 cgcgcgatgc cgaaatcgat gatcttggcg cgacgcggat cgccccccgg caggatcacg
     1381 ttgtcggaag aaatgtcgcg gtggacgacg cccagccggt gcgccgcatc gagcgcgctg
     1441 gccaatcggc gctgcaggat ccgcacttcg tcgatcggca gcggcccttg cgccaaccgc
     1501 ttgcctaaag aggggccgtc gacgaactcc atcgccagat aggcgcgctg caattcggga
     1561 tcgaccgaga agacgaagta gcggacgatc gcctcgtgat gcagattgag gagaaccgat
     1621 gcttcccgct tgaacatcgc gaaggcgtcc gggtcgcccg aaagctcggg caggatcatc
     1681 ttgatcgcga ccggatcgtt ggtgacgatg ttgaagccgc gatagacctc gcccatcccg
     1741 ccatgggcga tgagcggccc gatctcatag attccgttga ggcgcgcgcc ccggcgcacg
     1801 ctgcccgcag gcgccgccgg ctgaatgacc gtcctatctt ccacgcttca tcgctcccgg
     1861 tcgatgattt acgggccgcg gccggcctca ccatatcaga atcaagccag cgggcgaatg
     1921 gcccggaacg ttagaccgta agccgaagcg cagaaggctc gccggtcggc cggacttgcc
     1981 ggcgctttgg cgaacagacg ggagcaactc acatggtctt ctcgcggatg gctttactcg
     2041 gccgcctcgc ccgcagggat cgcggccatt ctattttcgt tgacggtctc gcggagcgcg
     2101 ccgatagtgc cgccgctcgc gcgctcaacc gcgagccgag tccagggagg cttctcatgc
     2161 gcgcttccat tgtcgccgcg ttgggcctgg ccgccggcct gaccgtccat ccggcttttg
     2221 cgggtcccgc ctacaaagct tcgaccgtcg ccgatttctt cgcgaaagcg gcgctcggca
     2281 aatcgcgggc gatttgcttc ggcaccgctg ccgattgtcc gcagccgccg cccgacggct
     2341 cggcgaagtt cgaccttttg gtgacgttcg aattcgattc cgacagactg accgccgccg
     2401 ccaaggaaaa tctcgaccag ttcgccgagg ccctgcacga tccgcgcttg aaaggccaga
     2461 agttcgagat cgacggccat accgacgcga ccggcaccga gcagtataat ctcggcctgt
     2521 cggagcgccg cgccgcctcg gtcatcgcct atctcgccac caagggcgtc gatgcgcgcg
     2581 aactgatcgg caagggcttc ggcaagacca ggccgcgcgt cgccgatccg ttcagcccgg
     2641 aaaaccggcg cgtcgagacg catttgctcg agcagccggg aggctgacgc accggccccg
     2701 ctcgccggtc ccgctcgaaa agcgggaagc gcgccgcaag cacattctct tcgacgcccg
     2761 cctgccgcgg cgtcggggcg cccgcgccgc cgtcccatcc aggatgatcg tacctcgccg
     2821 cgccgccgcc aagcggaccg taatcgccga aatgaccgaa acgttcgcca agcgccggaa
     2881 cggagatgac ctatgcccga tcccaaacag caaatcgacg cggcgctcgg cgacggctat
     2941 tacgacgaat ggaagcaaat ggatgtcgac ctcggtagcg agaacaatcg tttcgactcg
     3001 atccggcgcc tcctcaatct gcccttctct ccgatcttcg atcgcgccta tgacgatttt
     3061 cggaccgtca atgacattgc gcgggactat cagcagggga tgagcctaat tttggcgggc
     3121 cgcgcggtga acgtcggcaa gcttaaggag atccacgacc tctacacgct cgcgctgggc
     3181 gtcgcggaga acgacaacga tcttttggag gcgttttggg gcagcggctt tgccgcggcc
     3241 tgggtgatct tccccttcac gctgctgcaa gccaaatcga acgcaatgct cgccgcgttg
     3301 aaggagctga agaaggaatt ggcggaagcc gagcatgagg tgaataaggc cgtttggaag
     3361 cggcgcattc atctcgtcgt ctctttcgtg gaggcgatgt tcccggaaat ctctctcacc
     3421 gggcgcgccg cgctctatct cggcgacgtg atcctcgata aagcgctcgg accgaagaac
     3481 acgtcgacgg cgcaaaaata ttttggcgac gtcaatcccg gcgtgaagca attctccgag
     3541 gccgttcatc atatcgaagc gtatggcgag acggccaaag gcgtcttcga atggaccggc
     3601 aaggcctcga ccgtcgccac cttttatgtc gattttgaag agatcggcga gggcgaggag
     3661 cgggtcgaga agctaaaaag cctgatggag aaggtaaagg acgcctacga cgccctgcat
     3721 aagacgatcg aggacaacaa gtccaagctg atggcctttc tggcggcgtt cgatcgcgtc
     3781 gttctcgcca tcgaaagcgt tcgcatcaac ggagacaata tccgccaggc gctcgtcgac
     3841 gacgccgcga aataccgtta tcctcgcggc gccgcggcgt cctggctggc cgttccgtaa
     3901 ggcgtcattc ccggaaacga aggcggatcg gcgccggctt gcgccaggcc tcgtcctggc
     3961 gtttgatccc ttgagcccga cggcgcccaa ggaccgccga atcggcgcat agcgaaagag
     4021 aatgacgcgg cagaaagggc gcttttttct catcgcggcc ctggctgtgg ccgtcgcctg
     4081 cgcggcgctg atcggctttc gcttcatcaa gcgaagcagt gcgccgcctc ccccggcaag
     4141 cgtcgcaacg ccggccgcca atccggttaa gcccgccggt ccgatctcga cgatcatcga
     4201 tttgccgggc gatccggtcc tggtgcgccg cggtccgtcc gcggccctcc gctcggttca
     4261 catcgacgcg ccgattgcgc tgacgcccaa cgcggcgaaa ttcgacggcg acgcctattt
     4321 cgtcgcttcg ccgctcctat cgaccaatgg cggcttcatg ggcaagttcc cggagaccgg
     4381 cgaaggcgcc gaccaggcgg cgatgatcgc cgtcgccgac caggtcgatc agagcgacgc
     4441 ggcgatggag gcgggcttcg acgacgacgg cggcgcgaac acgccggctt acgccgatgc
     4501 gctgttgccg aacgccgcca atgtcagcca gatcgaagtc aaagccggcg gcgccaatgg
     4561 ccagccgcaa attaaggaga cggtgatcaa gacggtcgcc agcgagaagc tttccgacct
     4621 gctcatccgc gccggctaca gcgaggagag cgcgcgcgcg atcgaagcgg cgtccaaagc
     4681 gctctacaac atccaggcgc tgccggccgg cagcgtggcg atcgccgagg gcgccctcga
     4741 cgtctccggc gcgtaccgcg tagtgcaatt ggcgctttat gaaggcggcg aatatgtcgg
     4801 cgcggtcgcg ctggccgagg cgggcggcta tggcgaaggg gcgaagcccc cggtccctga
     4861 gggcgtcttc aacgaagggc agagcgcgcc cgcggcgccg gcgcaatata atctggccga
     4921 tgggctctac agcgccgggg tgcgcaacaa tgttccggag ccggtgatcc gcgaggcgat
     4981 ccaactgctt ggcaagatga cggacctgaa ggcgctcgct tcgccggagg cgacaatgcg
     5041 cctgatgttc tcgcgcgtct ttcgcggcaa gtccaagtca accggtcgga tcatctatgt
     5101 cggccttgtc ggcccgggcg gcgccgtcga ctgctatccc ttcgccggcg cagatggcga
     5161 atttcgctgc ttcaacccga agggcggcgc gccggcggcg ccgattccta cgccgcgccc
     5221 gggcgcgccc ccggtcggcg ccggcgaaat tttcccgccc atcaagggcg cgccgatcac
     5281 ttctctgttc ggcatgcgct tccatccgat cctgcacatc atgcggcttc acgccgggat
     5341 cgatttcggc gcgccgatcg gctcgatcgt gcgcgccgtc gccgatggca aggtcgagat
     5401 cgcaggcccg gtttccggct tcggcaatca tgtgcgcctc aagcataacg gcttcgagac
     5461 gtcgtactct catctgtcgg aaatccccgc gaatatcacg ccgggcgcga cggtcgtcgc
     5521 cggtcaggaa atcggtcttt ccggcaatac cgggctgtcg acggggccgc acctgcattt
     5581 cgaatattac ctcaacggcg tcgccgtcga tccgctgccg catatgaccg gcgaggcggg
     5641 cgcggcgatc gcctccgccg tcagcgcgcc gagcccgccc agcgagcagg aaatcgccgg
     5701 cttcaacacg gccaaggcga cgatcgacgc ggcgctggac gcggcgagcc gctgagttga
     5761 tctccccgcc aaagatggac gtgtcttttc tctttacaca actttaggtt gcatgctaga
     5821 cggatgccgg cgtgcggctg ggaggcgtcg atgtggttca agatcgaagg caagcgatct
     5881 tcgctcggcc agaaattcgt cgttcgctgc aagtctgagc tcgccggcaa agcggtgttc
     5941 gaggagcgca tcgtcaaggg cgcgcccgag gcgcgcaaga ccgcggaaat tctatccacc
     6001 tcgctcaacg tgacggcgac ggtctacggc gccgacgccg ccggcgaatt cgtctatggc
     6061 gtctacgaga tggcggagga ggcgagcgag gcgagcaatc cgatcatcaa gcgccttttc
     6121 gccgaatcgg cggccgcgtc cctgtctttc aaagacaggc tgctcgccga cattcccgag
     6181 gagacgatta ggggttttgc gactatggcg gcggcgctcg gctatttcgc cgcgcagcac
     6241 aagcagatca ggcggtcgag ttcaccccag atcagctatt cgaccctctt ccgcattttc
     6301 tgcggcggcg gcgaatcgag cacgttgacg ctcgcctcgg acgacgcgac gatcgcaatc
     6361 cttacgcaaa aactcgccga cctgcccggc tggatgtggc tcagcgaatt gcccggcctc
     6421 ggctcggcgg atcacggcat tgtcagccct tatgcgcggc cgaacaaagt cacgctcaat
     6481 atcgacggcg aattgcaagt gctgcggccg cgcaccgttt ttcgcggcgt cgcgccgggg
     6541 agcggcgcgc atttcatgct gatcgcgcag ctcgacgatc ggcacgggcg cccgcgccac
     6601 agtatcggcc ttgtctaccg cgaggccgac gaggcgtcga tcgtcgcgct tgtacgccgg
     6661 cttttgcagg aatcgaatcc gttcaaaaat cgcgtcgtgc tcatcaaccg cgatctgcgc
     6721 accgtgcgct cgcgcatgtc ggagcgtact tggcgcgaca tcatcccgca cgagcaagcg
     6781 cgcgccgagc tcaacttcat caccgcctcg atccgcaacc gcgacatgct gaaagcggag
     6841 ggtttgagcg tccggcgcgg cctgcttttg tccggcccgc cgggcgacgg caaatcgagc
     6901 gccatcgaat gtttcgtcaa tgacatcgcc aacgaggcga cgatcatcgt cgtcgaagcg
     6961 gtcgaccata tccgctcgct ctatcacctc gcccagatgc tggcgccgac cgtcgtcatc
     7021 ctcgaagacc tcgatctcat cacaaaagct cggcagaacc cctacgcgat cggcggcaag
     7081 gacgacgtca ccggcgaatt gctgcaggtc ctgtccggcg gctcggctta tgccgacatt
     7141 gtcaccatcg ccacgaccaa ccatcccgag gcgatcgacg acgcattgtc gaagcgggcg
     7201 ggccgcttcg acgcccatat ccgcatgggc tatccgagcg aagcggaaaa gcagcgcatt
     7261 cttgcgctct atctcgaccg ctatggcgtc gacgacgaac tgacgcgccg ccgcctacag
     7321 caaatgctga cgcgcgacct cggccggctg cacctcgttc ccgctcatat cgaggaattc
     7381 gtcaaagccg gcgtcaagcg cgcgcgcttg gctcgccgag ccccggaata tctcgatttc
     7441 gagcccggca tcgaagcgac caaatcgatt gcggcgtcca agccggcggc gtagatcgca
     7501 acgttccgcc atattgcgag taaaccgacg catccaattc atcgggtccg acgccctatg
     7561 accccccccg cgcgacgcgc gaaatcctcg caatgacgat cacccgccgc gcttcatctg
     7621 ctcgatcgcc tcctcggaca gcagctcctt caataggccg agaaagtcgc gcgaggcgag
     7681 cgatttggcg cggtcgagca gaaagggaac cgggctggac ggctcgactt tgcggaaatg
     7741 ggcggcgacg ccgtcgatga gcgcgatcgc ggcggcgcgg gaggcggccg gctccgtcgg
     7801 cgccggggca ggatcgaccg gctcggcggc ggggccgacg ctgctgaccg gaaccgagaa
     7861 agccggctcg gcgccgacaa agacccgggc ttgatcagcg taattcggcg ccaggacttt
     7921 catcacctcg taaatgtttt tgccaagcaa ttgccgcgcc tgcccgatca gcaagaccgc
     7981 cgcgctggac ggctcggcgc gggcgaaata gccgagcgcg ccggcgagcg cggcgtcgac
     8041 ttcggcgagc gaagcgaagt cgctcggcgc ggcggcggcc cccgcgccgg attcggcctg
     8101 gccggcctga ggcgcgggcg cttccggcgg cgcgatgctg ggatcacgcc gggcgaccgc
     8161 cgcctgcacg aaagccgcca tgcgctcggc gagcggctcc aaggcctcga aggtcggcgc
     8221 ctgctcgaaa ctccccttct cgatcgagac cgacctgatc tgggcgaccg ccgccttgat
     8281 cgcctggagc gcggcgagcg cctgtttcaa gccgtcgaga tcgcaattga gcaggatttt
     8341 ttcgatcgtc gcgctgtccg gcgcggtttc gccctcgcgc aaggcggcct cgccgagcgc
     8401 gatgagatgc gcgcggaaat tgactgcgcc gtcgcgctgc gtctcggcga gcggcgcata
     8461 ttgcagcggc aggatgacga ccggcccgtc gttcaatgtg gcgagctgcg ccatccgcac
     8521 ggcgaaatcg ccgtcctcgc cgcgcggatg aacctcgtcc caatattcgg cgatcaggcg
     8581 cgcggtctcg ctcagccaaa aggcgaagcc gcggagatcg cggtcgagaa tcgccatttt
     8641 ggcgagcagg acgatgaggc ggaggtcgag cgtgcgctcc agaagcttgc ggccgctggc
     8701 cagcgtctcg gcgaaatcga ccgtcttgcg gtcgaaggcg aaataggcgc tcggcaggcg
     8761 cccttccgcc gcagcgagga aattcatgaa ctcgccgtcg ccggccgcgt cgagatccgg
     8821 cccgcaaggc gcctctgccg agatcggcgc cagcgtaggc aatttgtcga gcgttgcggc
     8881 catggcggag ctcatggtta aagcggcttc gttcataacg agaggcccca agcccttcgt
     8941 acatcgaagg ccatcatagc cgaacccggc gctgcgggaa gctccgccgc gccttgccgg
     9001 cagcccgtaa gaacagcgcg ccagcttgac ggccttcggc ggcttaagat gtaatcgccg
     9061 tccggctcga agaccaggcg ccggtcacgc tggcggagat aagcgggcgc gacgatggcg
     9121 acggactacg attacgagct caatcccccc aggctcgccc ggcagaagga cctcgacacg
     9181 tgctgggcct gctgcatgtc cggcctgctc gccgccaaca tgtcgcgtcg gcaggccagc
     9241 gaggacgagc tcgtcaggac ttacgcgaca acgccgaccg gcggcatcga cgtctccaag
     9301 ctccgggtcg tggccaagga tttcagctat ttgtgcaacg acttccagac cgccgccgac
     9361 gcgcgctcga tcctgagcga ccgcttcgtc gtcgaccgcc tgcgtggcaa cggcatgctg
     9421 atggcggcct ggcgggtgca tgacccgaac aagccgaacg aggtgttttt ccacgcccag
     9481 atcgtctggg gcgtgatcta cctgtcgaac caggatgtcg gcaccgagcg cgcgctcatg
     9541 cgcacgatga atccgttcac cgcgaaatat gagctctatc ctctcttcac cgtctatcgt
     9601 tccgataata tgccattgtt cacatgctgg ccaagcacga gcgccccata atgcgggcag
     9661 accgtaaagc ctatcgggaa gaagtttgcg cgtcgaaatc gtcgcgcgat cgaaaatccg
     9721 cggcgcgaag cggtcttagg gagccttaag aggacgccat gagcctgtcg caggacgatc
     9781 gcatcggcaa attgaccacg ccgctcggcg aaaacgtgct ggtcctctcc cgcttggacg
     9841 gcggcgagca tttaagcgag ccgttcgagt ttcgcatcga ggcggtcagc gagcaacaga
     9901 acgtcgattt cagcggcgcg ctcggcaaag gcgtcaccgt caagctgaaa acctcggatc
     9961 acaaggaccg ctatttcaac ggcgttctgg ctgaagcgcg ctgggccggc ccgcgcgccg
    10021 atctcttcgt ctatcagctc gtgctgaggc cgtggctctg gctgctgtcg ctcacctcgg
    10081 attgccgcat cttcgcgcag aaatcgccga aagagatcat caagcaggtg ttctcggatc
    10141 gcggcttctc cgattttcgc gacgagctga aatcgtcgta cccgacgct
//
LOCUS       DATGJW010000049         3938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_838250, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000049 DATGJW010000000
VERSION     DATGJW010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3938
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_838250"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..731)
                     /locus_tag="VKU03_01405"
     CDS             complement(<1..731)
                     /locus_tag="VKU03_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69941.1"
                     /translation="MTGEAAKPAQERDLGTSREISTSAFPRQAATAEQSAASRERVAE
                     KTQIDTMSPPKDASRPAPAASVAEEGPVAASSAQPATAPATASSAATGPDGEGPSRGQ
                     AEHDPPYDFQYLPSPKDSSPQRPLASTPESSSTPPASSPLDIAASAKRASVDTAARRG
                     SDFGENAPPPSPAGAESSAKSSAPRDEAAPVRPAMRYDFAQIEHEGAASLSPSPAESA
                     TDASTRLEAPPEIAAPQGQAQLASGG"
     gene            1677..2438
                     /locus_tag="VKU03_01410"
     CDS             1677..2438
                     /locus_tag="VKU03_01410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69942.1"
                     /translation="MAADNPLPSLLPIAVLALAVTLCGSSPSLALGVGVGVGVGVGGH
                     GGATAGASVGASGVSAGASVGAAGTSAGIGASVGSNGANAGVGASNGATGTSAGIGAS
                     VGSTGANGGVGASNGATGTSAGIGASVGSTGANGGVGASTGATGTSASVGASAAASGS
                     SVGATGSASSDGAAADSSAAATAPVSSAFAATLDPLGPPPLSKNGRIAKLRLRCVVVR
                     AHPTVYDKDLVGLCAAIARRDARALRRAHARAAWR"
     gene            complement(2665..2913)
                     /locus_tag="VKU03_01415"
     CDS             complement(2665..2913)
                     /locus_tag="VKU03_01415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69943.1"
                     /translation="MLILTKLMILLASLFSGYDAYSVLVIVPLGAAALVVFFAARPEA
                     LANGWSKGGVSYVADMTARNCVLVGVLFGLGRLLSAAV"
BASE COUNT          600 a         1240 c         1445 g          653 t
ORIGIN      
        1 ccgccgctgg caagttgcgc ctgcccttgc ggcgcggcga tctcgggcgg cgcctcaaga
       61 cgcgtcgagg catcggtcgc tgactcggca ggcgaaggcg agagggatgc ggcgccctcg
      121 tgttcgattt gggcgaagtc atagcgcatc gccggccgaa ccggagcggc ttcgtcacga
      181 ggggcggagc tttttgcgga gctctcagcg ccggcggggg atggaggcgg cgcgttttcg
      241 ccgaagtcgc tcccacgccg cgcggccgta tcgaccgatg cgcgctttgc gcttgcggcg
      301 atatcaagcg gcgagcttgc cggcggggtc gatgacgatt cgggcgtcga cgccagcgga
      361 cgttgcggcg aagagtcttt cggcgatgga aggtactgaa aatcgtacgg cggatcgtgt
      421 tcggcttgcc cgcgcgacgg tccttctcca tccggcccgg tcgcggccga ggaggccgtc
      481 gcgggcgccg tagcaggctg cgccgatgac gccgcaaccg gaccttcctc tgcgaccgac
      541 gcagccgggg ccggacgcga cgcgtctttc ggcggcgaca tggtatcgat ttgcgtcttt
      601 tcggcgactc gttcccgcga ggccgccgac tgttccgcgg tcgcagcctg gcgcgggaaa
      661 gctgaggtag aaatttctcg cgacgtaccg agatcgcgct cttgcgccgg ctttgccgct
      721 tcgccggtca tcgccaatgc cggattttgc gaccgcggcc ctgacgtgga tcctattgct
      781 gggacagccg ccttcgccgc ctctgactcg gccgcgcgag ccggcgcgcc ggaagggtca
      841 gaatggttca tctccggcgc gctggctggc ggggtcgcgc tcgcgtcgtc gtgcggcgtc
      901 gtcgcgcgcc gcgcctcttc gggcgacggg tggcaagcct ttttgcgagc atcgagatcg
      961 gattcaagat cgccaatccg aaggaacgcg tcattggctt ctttgaattt gttgtcgtgg
     1021 atgagctcaa tcgcttcggc gacgagccgg tcgcgctttg gaagaaagat caaactgaga
     1081 ttggctcggc acagcgtttc gagcgaatcg acgacgctcg cgctttttgc ggcgcgaaag
     1141 gcggcatgcg tccgggcgat aacttgatcg gcgctcgacg tctgcgcgcc ggcggcggaa
     1201 acctgaacgg cgaaggcgat cgccgcgatg ttgcgcctcg tcgcgccata acggcgcttg
     1261 cgcggaattc ggttcgcctg cgccatttgc ttaagctcca tgtcgcgtta tggacctgtc
     1321 attgcgcgcg gcgaagtttc ggcgtcttcg gaagcctccc aaagccgctc ttgcaaaaac
     1381 ttcagccgtt ccgccagcgg cgccatgtcg atcgcgctcg ctcctgcaaa tggaacgctt
     1441 gtcggctctc gcgtaaaagc gtggcggtgc gcccctcatc cggaatggcg atagccgcta
     1501 acaacccgac gagtataaaa cgaacgccgg gaaaatgcgc tggaacgaag aacgcgcgaa
     1561 ggggctacct cttcagcgcc gacgagcgcg atcgcaattt gtgtcggcaa tatggaacta
     1621 tgtcgacgcg tgccgatttt gactacaatt tagcgaaatt tcgacgggag cgtgaaatgg
     1681 ccgcggataa tcctctccct tcactcttgc cgattgcggt tctcgccctc gcggtgacgt
     1741 tatgcggctc ctcccccagc ctcgctctcg gcgttggcgt tggcgttggc gtcggggtcg
     1801 gcggccatgg cggcgcgacg gctggcgcga gcgtcggcgc gtccggggtc agcgccggcg
     1861 cgagcgtcgg cgcggccgga acgagcgccg gaatcggcgc cagcgtcggc tctaatgggg
     1921 cgaatgccgg cgtcggcgcc agcaatggcg cgaccgggac gagcgccgga atcggcgcca
     1981 gcgtcggctc gaccggggcg aatggaggcg tcggcgccag caatggcgcg acggggacga
     2041 gcgccggaat tggcgccagc gtcggttcga ccggggcgaa tggcggcgtc ggcgccagca
     2101 ctggcgcgac cgggacgagc gccagcgtcg gcgcgagcgc ggcggcgtcg ggttcgagcg
     2161 tcggcgcgac ggggtcggcg tcaagcgatg gcgcagccgc agactcttcg gccgcagcta
     2221 cagcgccagt ctcatcggct tttgcggcaa cgcttgatcc actcggtcca cctcctctct
     2281 cgaaaaacgg ccgaatcgca aaattgagat tgcggtgcgt cgtcgtgcgg gcgcatccga
     2341 ccgtctatga caaagacctg gtcggcctat gcgccgcaat cgcccggagg gatgcgcgcg
     2401 cgttgaggcg cgctcacgcc cgcgcggctt ggcgctgaca atccaagcac gccgcttcgg
     2461 ggtctgggcc ggcctggcga cggtccgacc cgggtatcgc gcgtccgcct caagagccgg
     2521 aatggcgcga ccgctgcttt tctgaaacat cgccgcgcat cggcaagatc aactttaaga
     2581 ccttgccggg ccggcctgtg tgggacatag ggatcttttc ggggctctcc cggggcaacc
     2641 aaatcgcgag cgtcatccaa ttgcttagac cgccgcgcta aggaggcggc caagcccaaa
     2701 taaaacgccg accagcacac aatttctggc ggtcatatcc gcaacgtatg aaacgccgcc
     2761 cttactccag ccattcgcca acgcctcagg ccgggccgcg aagaaaacga ccagcgcggc
     2821 ggcgcccaac ggtacgatga ccaaaaccga gtatgcgtcg taacccgaaa agagcgacgc
     2881 gaggagtatc atcaattttg taagaatgag catttgctcc tcccggccaa aacacggagg
     2941 tcggcgcggg acttgagaga tccttatcat cgctggttat ttgatcaaaa tgatggcgac
     3001 ggatcgggtg tggccaggag ccaaagcgtc tcagcaccgc gcgaccgaac ggcgtccttt
     3061 gagaaacgtc ccgaaccgtg gaggctcggc ctagtctagc caatcgacgt agccccagag
     3121 cggccttccg ggacgccttc gctcgtgcgc gtaaggtcgc gccaaacgcg ccttaatcgg
     3181 ctgcccatgc aaaaccgcgc cctgggaagg gcgcggtccg gacgctcatc acttcggaca
     3241 aggcggttcg gatttgccgc cgcaggccgg cctcgggtga gcggcaggag ctggcgcggg
     3301 gtgtggcgcc ggcggcggcg cggcgtgaag aggcggcggc gctggcggtg gggcggcatg
     3361 gaccacgggc ggcgcggccg tctgcggcgg cgcatgcacg accggcggcg ccgctgggtg
     3421 cggcgtcggc ggcggggcgg catgaaccac gggcggcgcg gccgtctgcg gcggcgcatg
     3481 cacggctggc ggcgcggccg tatgcggcgt cggcggcggc gcgagatgag ttgccggtgg
     3541 ggcaatatga gcggccggcg gcggggccgg cttgcgctcc ggcgcaacat cgccgccggg
     3601 tggcttcgac ggctgtgtcg gcgcgacagg ccgcggcagg gcgtgtccgg caggggtctg
     3661 cggctggggt ttcggcggcg gtgcggcgtg gccggcggcc ggcgctggct gtggcgctgc
     3721 aggctgtggc tgggcgtggc cagccggcgc ctgcggctgc ggcgcggcgt gaccgcctgg
     3781 cgcgggggcc ggcggcggca gggcgccgcc ggccggcgcc tggggcggcg gggccgcgtg
     3841 attgcccgtg ggggacggcg ccggctgcgg cagagcgtgg cctgccggag cttgcggttg
     3901 cggcggcgcg gcgtggccgc ctggagtcgg ttgcggcg
//
LOCUS       DATGJW010000050        16545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_112263, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000050 DATGJW010000000
VERSION     DATGJW010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 16545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16545
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112263"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2331
                     /gene="malQ"
                     /locus_tag="VKU03_01420"
     CDS             <1..2331
                     /gene="malQ"
                     /locus_tag="VKU03_01420"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HLJ69944.1"
                     /translation="DRVAIALNRGHDDVDIALPTPRAGLAWRILLDSSALDRRDWDIA
                     PEEKPACPARTSMILAESAPQPRGARASDPALVDKLAEAAGIAGEWWDIEGKRTIVGR
                     ETKIALLKAFRLPAATNAEARDSLQRLVDERQRRPLPFSFVARDGESAYVPLRLDPKN
                     PNAPPIRLNCEGGREIALAPDVEAQRVTLADGHEIVERRILLPALPIGRHKLIAGDAA
                     CDLAVAPAKAHLPDMLRRRVFGVSAQLYAQRRLGDQGIGDFSTLGRLGAAAGAAGAAL
                     LGVNPLHMLFAQDRERASPYHPSDRRFLDPIHVDALDGVLLPSDAGFVELALERAQEL
                     AGLARAGAVDYPRVWRVKSALLESRFAAFRKARAAQPRDPLFAEYDRFIAAGGETLER
                     FACFQALAEERRGEDWRQWPDDLRDGAPAALRGATERLRERVDYHAFLQWLADRQLAG
                     AASRGKAGGLSLGLYRDLAIGAAPDGAEAWARAHELGQGVSVGAPPDPFSRDGQIWHL
                     PPPDPLAMARDAWRGFSALLAANMRHAGLLRLDHAMGLTRLFLVPDGAKPSDGAYVSY
                     PLQDLIGLLALESRNNQCAVVGEDLGTVPEGFREKLTEAGIFGMRVLWFEKDDDAFRA
                     PAAYPALAVACAATHDLPTVAGWWAGADIAERKALGLIGAESAQSQIEAREREKRALA
                     EALVAAGLLREAPDPTAPASDALAAAIHAFVARAPSLVALAQADDLAGETIAPNLPGT
                     DRQRPNWRRKLPDDAGALFSSARAQAIIAALKTERP"
     gene            2454..4184
                     /gene="araD"
                     /locus_tag="VKU03_01425"
     CDS             2454..4184
                     /gene="araD"
                     /locus_tag="VKU03_01425"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="L-arabinonate dehydratase"
                     /protein_id="HLJ69945.1"
                     /translation="MGAQKKRPEDLRSFRWFGPQDLRSFGHRSRLLQMGYGAQDFAGK
                     PVIAIINTWSDINPCHAHFKARVEDIKRGVLQAGGFPIELPAMSLSETFVKPSTMLYR
                     NFLAMETEELIRSHPVDGVVLMGGCDKTTPALLMGAISADLPAIFMPAGPMLRGAYRG
                     ESLGSGSDVWKYWDEKRAGKISDEEWREMEAGIARSYGVCMTMGTAATMTALAEVLGM
                     TLPNASSIPAADSAHIRMAAETGRRAVEMVFEDLTPSAILTAASFDNAIVALMALGGS
                     TNAIIHLVALARRAGVALDLDRFDEIARRTPVIANIRPTGQFLMEDFWYAGGLKAFLS
                     TLAPLLDMGQRTVNGNSLAENIAGAKVWRDEVMRPLNDPVAGSDGLAVLKGNLAPRGA
                     VMKPAAAEPRLRRHRGPALVFASYDEMAKAVERDDLAITPDHVLVLQNAGPQGAPGMP
                     EWGMLPIPKKLVRDGVRDMLRISDARMSGTSYGACVLHVAPEAYIGGPLALAKTGDMI
                     SVDVDKRSLTLDVSDDELARRRAGFVPRPPRLARGYGALFARAIGQADEGCDFDFLLR
                     GEKLPEPEIH"
     gene            4344..5486
                     /locus_tag="VKU03_01430"
     CDS             4344..5486
                     /locus_tag="VKU03_01430"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HLJ69946.1"
                     /translation="MSVYELARIPRVTAGIGAREKIGALAASEAKSHALLVADPGVKA
                     IGLIDETAALLRKSGVSFTLFDSFQSDPTVVQADAAAEIARREKASVVVCLGGGSALD
                     LGKTVAAIALAPHSAGHYALAAQPLPPRGLKKICAPTTSGTGSETTRTAILTRDDHAK
                     VWLWGDEIKADEVVLDPELTASLPGPLTAATGIDALVHAVEAATNRNANLAAGLFAHQ
                     AIRLVGRHLLSAIENPRDLAAREALQWAAAYGGIAIDNCGTAIAHALGHAMGSLRPIH
                     HGRAVGIAMLASLPWNVEGNDAFAACAADLGGERSSAGFIAAYEKLLRATGMKLSLAE
                     DFAGITAADLAEQTARPENDAMRASNRRVSNKTDLLKLADAVLSVA"
     gene            5591..6739
                     /locus_tag="VKU03_01435"
     CDS             5591..6739
                     /locus_tag="VKU03_01435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007690100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLJ69947.1"
                     /translation="MTDRFQPLASAPQATITAIEITRHRLHFDPPFNASWDTKPRTYW
                     DAAIVRVHTDLGVTGIGSGDMMLGFEGHEPLFIGQDALAIERHWRVLNNISFHWGRCW
                     PLDIALWDLAGKIAGQPCWKLIGGLSDRVKAYASSGTLRGPAELADAAEAYLAMGFSA
                     LKIRFHRGDWRADIKALEAVRGRIGDRLDLMVDCNQGWRMSWDTYAPWSLKDAVKVAR
                     ELERLGVYWMEEPLHRGDRDGMRRLRDMTDIRIAGGEMTRELYEFRDLIAEGCLDVLQ
                     PDAALVGGLTGLRRVALMAQEHNLVFTPHTWTNGIGVVANAHLTAGLADAPFVEYPYD
                     PPEWDVDRRDYMMREPLKISADGNIVLNDRPGMGYDLDEERLAKTRIG"
     gene            6786..7787
                     /locus_tag="VKU03_01440"
     CDS             6786..7787
                     /locus_tag="VKU03_01440"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLJ69948.1"
                     /translation="MDRKWLPLNALRAFEAAGQHLSFTAAANSLTVAQSAVSRHVIVL
                     ENFLGVTLFERRPQQLVLTEAGRHILPVLTKSFDRIDQALSEVIKEKGRPKRVLKVAL
                     PTTFAHRLAIPILRDFRAEHPGITLEIVSKPTSGAEVDGDVDIAIVYSEPRVTDAILD
                     LLWMVRSTILCSPKLLQGVERIDPARLIASCDLLHVKVDDRPRHFFWEMLTRSLGRPD
                     LPVDRGLVFDTAQLAVQYALSGDGLVLADPMLFQEEVRSGRLVRPFDLGLEEGYGYYL
                     TTHPEDLNNEAVALFRSWLIARFSAETAEAAAAPTAPATPPAADMPEAERPKLRAAK"
     gene            7884..8297
                     /gene="arfB"
                     /locus_tag="VKU03_01445"
     CDS             7884..8297
                     /gene="arfB"
                     /locus_tag="VKU03_01445"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003747 - translation release factor
                     activity [Evidence IEA];
                     GO_process: GO:0006415 - translational termination
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alternative ribosome rescue aminoacyl-tRNA
                     hydrolase ArfB"
                     /protein_id="HLJ69949.1"
                     /translation="MQVGRINIDESEISETFVRASGPGGQNVNKVSTAVELRFDARGS
                     KSLPDDVSVRLQRLAGSRLTQDGIVVIFAQRFRSQERNRADARERLAALLMQAQKRPT
                     VRRPTRPTKASKEKRLESKSLRGRIKNLRRGPLDE"
     gene            complement(8323..8559)
                     /locus_tag="VKU03_01450"
     CDS             complement(8323..8559)
                     /locus_tag="VKU03_01450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDCCVxC domain-containing (seleno)protein"
                     /protein_id="HLJ69950.1"
                     /translation="MQLNSVVTCPVCRHQALETMPTDACRVFYECKSCGALLKPKPGD
                     CCVFCSYGSAPCPPIQAERAACSAASCRRPGDQT"
     gene            complement(8603..9001)
                     /locus_tag="VKU03_01455"
     CDS             complement(8603..9001)
                     /locus_tag="VKU03_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLJ69951.1"
                     /translation="MASIRKELQLDADAADVWAALADFGAVDTRVAPGFVVSSRLDGD
                     RRFVKFANGVEAIETLVSRDDESRRLVYAILGGRMAHYNAALRVFAEGAGRSRLVWIV
                     DLLPDDFAAYIDGQMEAAFKAMKPRLEARS"
     gene            complement(9006..9881)
                     /locus_tag="VKU03_01460"
     CDS             complement(9006..9881)
                     /locus_tag="VKU03_01460"
                     /EC_number="1.1.1.157"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxybutyryl-CoA dehydrogenase"
                     /protein_id="HLJ69952.1"
                     /translation="MAINKIGVIGAGQMGNGIAQVCAVAGLNVVMSDVTEDRLKAALA
                     TINGHLAREVARGQIDEAKRHEALSRIDTTTDLDKLSDCDLVIEAASENEEVKRKIFT
                     NLRPYLKSDAIVASNTSSISITRLASITANPERFIGIHFMNPVPRMQLVELIRGIATQ
                     NETFETAKAFVSRLGKTATLSEDFPAFIVNRILLPMINEAIYTLYEGVGTVEAIDTAM
                     RLGANHPMGPLQLADFIGLDTCLAVMQVLHEGLADSKYRPCPLLVKYVEAGWLGRKTQ
                     RGFYDYRGEHPMPTR"
     gene            complement(9958..10902)
                     /locus_tag="VKU03_01465"
     CDS             complement(9958..10902)
                     /locus_tag="VKU03_01465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLJ69953.1"
                     /translation="MATLLLAEVANGHLADIVAKALTAAAQLGEPVDILVAGRNVGAA
                     AEAASKLAGLRKVRVADDPRYEHGLAEPLAALVVSLAGDYSAIVAGSSAYAKNVMPRV
                     AALLDVMQVSDIIKVVSPDTFERPIYAGNAIQTVQTSEPKKIVTVRTASFQATGAGGP
                     APVEPVAAAPDPGLSVFKGEALAKSDRPELTSAKIVISGGRAMQNAENFKKYIEPVAD
                     KLGAAMGASRAAVDAGYAPNDWQVGQTGKVVAPDLYIAVGISGAIQHLAGMKDSKVIV
                     AINKDEEAPIFQVADYGLVADLFTALPELAEELSKTGR"
     gene            complement(10904..11653)
                     /locus_tag="VKU03_01470"
     CDS             complement(10904..11653)
                     /locus_tag="VKU03_01470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013761780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HLJ69954.1"
                     /translation="MKILVPVKRVVDYNIKIRVKADGTGVELANVKMSMNPFDEIAVE
                     EALRQKEAGKASEVVIVSIGAAQAAETIRTALAMGADRGVLIKTDQIVEPLAVAKLLK
                     GVVEAEQPGLVILGKQAIDDDCNQTGQMLAALLGWGQGSFASKVEIGEGAVAVTREVD
                     GGLQTVELKLPAIVTTDLRLNEPRYASLPNIMKAKKKPIEEKAPADFGVDVAPRLKVL
                     KTVEPGGRKAGVKVKSVAELVAKLKDEAGVI"
     gene            11975..13555
                     /locus_tag="VKU03_01475"
     CDS             11975..13555
                     /locus_tag="VKU03_01475"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ69955.1"
                     /translation="MNELNCQREEPRIGAPSRREFLGGAAALGCSGYAASSRDAAAAE
                     TIKKGGLLRLGLAGGGPSDSFDPASYVDAVMIVAGRGLFNGLVEWTAEGGVKPELAAN
                     WEPRDGAKTWIFNLRKGIKFSNGQEFTAEDAVASLNRGRVGKSLASRDSTPIAEIKKA
                     DKYQVQVTLETPDADFPYVLTDCRLLMAPAGFKDWSKPIGTGAYRLDKFEPGVRVSLK
                     RAPDYWRAGEGRGNAEAAEITVLNELSERVNALVAGHIDVINRVDPRTVALLQKTPKI
                     ETLFAASGWRPVMAMAVDQPPFNNADFRLAMKYAIDREQMIRALYAGYAALGNDHPIP
                     PNDPYFNKELPQRKHDPDRAAFYLKRSGLSGAEIALEVSPAAFAGAPEAASLLQASAK
                     QAGVRMQIKSEPAEGFWRNVWLKAPFVESYWRGRPAAVHMLAIAFGADSPLNETHWRS
                     DKFEKLLADARSETEEAKRKPYIWEMQAMLHDEGGAIVPLFRDWIDARRDAVGGYAAR
                     GGADLANGYILEKAFLKA"
     gene            13921..16188
                     /gene="ptsP"
                     /locus_tag="VKU03_01480"
     CDS             13921..16188
                     /gene="ptsP"
                     /locus_tag="VKU03_01480"
                     /EC_number="2.7.3.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008965 - phosphoenolpyruvate-protein
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate--protein phosphotransferase"
                     /protein_id="HLJ69956.1"
                     /translation="MMRAPGGPRLLLRRLREVMAEPVTAQARLDRIVVLIASNMVAEV
                     CSLYVARADGPLELFATEGLKREAVHVTTMRPGEGLVGLIASTAEPLALSDAQSHPAF
                     SYKPETGEEIYQSFLGVPVLRGGATLGVLVVQNRARRTYSEEEVEALQTTAMLLAELI
                     ASGELQSIIRPGVDIALRRPVALKGAPIAEGLGLGHVVLHQPRVTVSKLVADDIPAEI
                     ERLDRAIEAMRASVDELIGRTESAGDGEHRDVLEAFRMIAHDRGWLRRLNEAVMSGLT
                     AEAAVERVMNDARARLQRQTDPYLRDRLHDLSDLANRLLHQLAGQSLILDPSELPENA
                     ILVARSMGPAALLDYDRQRLRGLVLEEAGAASHVAIVARALSIPAVSEVANISEIAEQ
                     GDAIIVDGGVGEVQLRPSADVEASYAEKARLRARRQEQYRELRDTPAITRDGVAIDLH
                     MNAGLIVDLPHLDESGAQAIGLFRTELQFMLAARFPRLADQQQLYETVFNAAGERPVT
                     FRTLDIGGDKVLPYMKGPEEENPALGWRAIRIGLDKPALLRTQLRAMLRAGADRNLRI
                     MLPMVASVDEFVAARALLERELAFNDRCGRRPPADIKLGVMVEVPSLLWQLDEIAERA
                     DFLSVGSNDLMQYLYAADRDNRRVADRFDPLSAGFLRALAAIAEAGRNAGKPVTLCGE
                     IGGRTLEAMTLIAIGYRGLSMSAASIGPVKAMVLSLDAAAATEATAELLARSGEAASL
                     REPLTALAERLGVRL"
BASE COUNT         2720 a         5599 c         5439 g         2787 t
ORIGIN      
        1 gaccgcgtcg cgatcgcgct caatcgcggt cacgacgatg tcgacatcgc tctgccgacg
       61 ccgcgcgccg gcctggcctg gcgcatcctt ctcgattcga gcgcgcttga tcgccgggat
      121 tgggatatcg cgccggagga gaagcccgcc tgtccagcgc ggaccagcat gatccttgcc
      181 gaatctgcgc cgcagccgcg cggcgcgcgc gccagcgatc cggcgcttgt cgacaaactg
      241 gccgaggcgg ccggaatcgc cggggaatgg tgggacattg aagggaagcg cacgatcgtc
      301 ggccgcgaaa cgaaaatcgc gctgctcaag gcgttccggc tccccgccgc gaccaacgcc
      361 gaggcgcgcg acagcctgca gcgcctcgtc gacgagcgac agcggcggcc cctgcccttc
      421 tctttcgtcg cacgcgacgg cgagagcgct tatgtcccgt tgcgcctcga tccgaagaat
      481 ccgaacgcgc cgccgatcag gctcaattgt gagggcggac gcgagatcgc gctcgcgccg
      541 gacgtcgagg cccagcgcgt cacgctcgcc gacgggcatg aaatcgtcga gcgccgaatc
      601 ctcttgccgg ccctgccgat cggccggcac aagctgatcg ccggggacgc ggcctgcgat
      661 ctcgccgtcg cgcccgccaa ggcccatctg ccggatatgc tgcgccggag ggttttcggc
      721 gtcagcgcgc aactctatgc gcaacgccgg ctcggcgacc aggggatcgg cgacttttcg
      781 acgctcggac ggttgggcgc ggccgccggc gcggccggcg cggctttgct cggcgtcaac
      841 ccgctgcaca tgctgttcgc gcaggaccgc gagcgggcga gtccgtacca tccgtccgac
      901 cggcgctttc tggacccgat ccatgtcgat gcgctcgacg gcgtcctgtt gcccagcgac
      961 gccggcttcg tcgaactggc cttggagcgc gcgcaggagc tggccgggct cgcgcgcgcc
     1021 ggcgcggtcg actatccgcg cgtctggcgc gtcaagagcg ccttgctgga gtcgcgcttc
     1081 gccgcgtttc gcaaagcccg cgccgcccag ccgcgcgatc ctctcttcgc cgaatatgac
     1141 cgcttcatcg ccgccggcgg cgaaacgctt gagcgcttcg cctgtttcca agcgctggcc
     1201 gaggaacgcc gcggcgaaga ttggcggcaa tggcccgacg atctgcgcga cggcgcgccg
     1261 gcggctttgc gcggcgcgac ggagcgcctg cgcgaacgcg tcgattacca cgctttcctg
     1321 caatggctgg ccgatcgcca gctcgccggc gcggcttcgc gcggcaaggc gggcggcctc
     1381 agcctcggcc tctatcgcga cctcgcgatc ggcgcggcgc cggacggggc cgaagcctgg
     1441 gcgcgggcgc acgagcttgg gcagggcgtg tcggtcggcg cgccgcccga tccgttttcg
     1501 cgcgacggcc agatttggca tttgccgccg cccgacccgc tcgccatggc gcgcgacgcg
     1561 tggcgcggct tttccgccct cctcgccgcc aatatgcgcc atgccgggct gctgcggctc
     1621 gaccacgcca tgggcctgac ccgcctcttc ctcgtgccgg acggcgccaa gccctcggac
     1681 ggcgcctatg tctcctatcc cttgcaggat ttaatcggcc tgctcgcgct cgaaagccgc
     1741 aacaatcaat gcgccgtggt cggcgaggac cttggcacgg tcccggaagg ctttcgtgaa
     1801 aagctgaccg aagccggcat tttcggcatg cgcgtcctat ggttcgaaaa agacgacgac
     1861 gccttccgcg ctcccgcggc ctatccggcg ctcgccgtcg cctgcgccgc gactcacgat
     1921 ctgccaaccg tcgccggttg gtgggcgggc gccgacattg ccgagcgcaa ggcgctcggc
     1981 ctgatcggcg ccgaaagcgc gcaaagccag atcgaagcgc gcgagcggga aaagcgcgca
     2041 ttggccgagg cgctcgtcgc cgccgggctc cttcgcgaag cgccggaccc aaccgcgccg
     2101 gcttcggacg cgctcgccgc ggcgatccac gctttcgtcg cgcgcgcgcc gtcgcttgtc
     2161 gctttggcgc aggccgacga ccttgctggc gagacgatcg cgcccaacct cccgggcacc
     2221 gaccgccagc ggccgaattg gcggcgcaag ctccccgacg acgccggcgc cttgttctcc
     2281 tcggcgcgag cgcaggcgat catcgccgcc ttgaaaacgg agcggccgtg atggttatga
     2341 cgagtcgccg tcattgcgaa aagcgaaacg gcgaagccat cccctcatag ggcgatgtcc
     2401 tacgaatgga tggctttgct tcgctcgcaa tgacgcggtg tgatggagca gcaatgggcg
     2461 cccaaaagaa acgtcctgaa gatttgcgca gcttccgctg gttcggcccg caggacttgc
     2521 gctcgttcgg ccatcgctcg cgcctgcttc agatgggcta cggagcccag gatttcgccg
     2581 gcaagccggt gatcgccatc atcaacactt ggagcgacat caatccctgc cacgcccatt
     2641 tcaaggcgcg cgtcgaggac ataaagcgcg gcgtgctcca ggccggcggc tttccgatcg
     2701 aattgccggc gatgtcgctc tccgagacct tcgtgaagcc ctcgaccatg ctctatcgca
     2761 acttcctggc gatggagacc gaggagctga tccgctcgca cccggtcgac ggcgtcgtgc
     2821 tgatgggcgg ctgcgacaag acgacgcccg ctctcctgat gggcgcgatc agcgccgacc
     2881 tgccggcgat cttcatgccg gccggcccga tgctgcgcgg cgcctatcgc ggcgagagcc
     2941 tcggcagcgg ctcggacgtc tggaaatatt gggacgagaa gcgcgccgga aagatcagcg
     3001 acgaggaatg gcgcgagatg gaggcgggga tcgcccgctc ctacggcgtc tgcatgacga
     3061 tgggcaccgc cgcgacgatg accgcgctgg cggaagtcct cggcatgacg ctgcccaatg
     3121 cgtcctcgat tcccgccgcc gattcggctc acatccgcat ggcggcggag accggccggc
     3181 gcgccgtcga aatggtcttc gaggatttga cgccctcggc aatcctcacc gcggcctcgt
     3241 tcgacaacgc cattgtcgcg ctgatggcgc tcggcggctc gaccaatgcg atcatccatc
     3301 tcgtcgcact ggcgcggcgg gcgggcgtgg cgctcgatct cgaccgtttc gacgagatcg
     3361 cccgccgcac gccggttatc gccaatatcc gaccgaccgg acaattcctg atggaggatt
     3421 tctggtacgc cggcggcctc aaggcgtttc tgtcgacgct cgccccgctt ctcgacatgg
     3481 ggcagaggac cgtcaacgga aatagcctcg ccgaaaatat agccggcgcg aaagtctggc
     3541 gagacgaggt catgcgcccg ctcaacgacc cggtcgccgg cagcgacggc ctcgccgtcc
     3601 tgaaaggcaa tctcgcgccg cgtggcgcgg tgatgaagcc ggccgcggcc gagccacggc
     3661 tgcgccgtca tcgcggcccg gctctggtct tcgccagcta tgacgagatg gccaaggcgg
     3721 tcgagcgcga cgatctcgcc atcacgcccg atcacgtcct cgtcctccag aacgccggcc
     3781 cgcaaggcgc gcccggcatg ccggaatggg gcatgctgcc gattccaaaa aagctggtac
     3841 gcgacggcgt gcgcgacatg ctgcgcattt ccgacgccag gatgagcggc acgagctacg
     3901 gcgcctgcgt gctgcatgtc gcgcccgagg cctatatcgg cggcccgctg gcgctggcca
     3961 agaccggcga catgatcagc gtcgatgtcg acaagcgcag cctgacgctg gacgtttccg
     4021 acgacgagct cgcgcgccgc cgggccggct tcgtccctcg cccgccgcgc ctggcgcgtg
     4081 gctacggcgc gctcttcgcc cgcgccatcg gccaggccga cgagggctgc gatttcgatt
     4141 ttctgctgcg cggggagaag ctgcccgagc cggagatcca ttgaggccgg cggcaggccg
     4201 aatggattgc ctcgcttcgc tcgcaatgac aggctggccg ccatgccaat ttgcgattgt
     4261 cgcatcttca aaaggctatt ccgccgctcg acgcggcggg acatggtgcc gcggccccgc
     4321 ttcacgcttt gacgagcctt acaatgagcg tctacgaact tgcgcgcatt ccgcgcgtca
     4381 ccgccgggat cggcgcgcgg gagaagatcg gcgcgctcgc cgcaagcgaa gcaaaaagcc
     4441 acgccctgct cgtcgccgat cccggcgtca aagcgatcgg gctcatcgac gagactgccg
     4501 cgctcctgcg caaaagcggc gtctcgttca cgcttttcga ctcctttcag agcgacccga
     4561 ccgtggtcca ggccgacgcc gcggcggaga tcgcgcgccg tgagaaggcg agcgtcgtcg
     4621 tctgcctcgg cggcggctcg gcgctcgatc tcggcaagac cgtcgccgcg atcgcgctgg
     4681 cgccgcattc ggccggccac tatgcgctcg ccgctcaacc gctgccgccg cgcggcttga
     4741 agaaaatctg cgcgccgacc acgtccggga ccgggtcgga gacgacgcgc acggcgatcc
     4801 tgacgcgcga cgaccacgcc aaggtctggc tgtggggcga cgagatcaaa gccgacgagg
     4861 tcgtgctcga tcctgaattg acggcgagcc tacccgggcc gctaaccgcc gcgaccggaa
     4921 tcgacgcgct ggtccacgcc gtcgaggcgg cgaccaatcg caacgccaat ctggccgccg
     4981 gtctcttcgc tcatcaagcg attcgcctgg tcggccgcca tctcctcagc gctatcgaga
     5041 acccgcgcga tctcgccgcc cgcgaggccc tgcaatgggc cgccgcctat ggcgggatcg
     5101 ccatcgacaa ttgcggcacg gccatcgccc atgcgctcgg ccatgcaatg ggctcgctac
     5161 ggccgatcca tcatggccgt gcggtcggca tcgccatgct cgcgagcctg ccctggaatg
     5221 tcgaaggcaa cgacgccttc gccgcttgcg ccgccgatct cggcggcgag cggagcagcg
     5281 ccgggttcat cgccgcctat gagaagctgc tgcgcgcgac cggcatgaag ctgtcgctcg
     5341 ccgaggactt tgctggaatc accgcagccg accttgccga gcaaaccgcg cggcccgaga
     5401 atgacgcgat gcgcgcttcg aaccggcgcg tgtcgaacaa aaccgatctg ctgaagctcg
     5461 ccgatgccgt attgtccgtt gcttaggagc cgagatcgtc attgcgagca aagcgaagcg
     5521 atccattcct agagcgccca aacggattgc tttgtcgctt cgcttctcgc aatgacggcc
     5581 gatatcgccg atgaccgatc gcttccagcc tctcgcatcc gccccccaag cgacgatcac
     5641 ggcgatcgag atcacccgcc atcgccttca tttcgatccg ccgttcaacg cgagctggga
     5701 caccaagccg cggacctatt gggacgcggc gatcgtccgc gtccataccg atctcggcgt
     5761 gaccggaatc ggctcgggcg acatgatgct cggcttcgag ggccatgagc cgctcttcat
     5821 cggccaggac gctttggcga tcgagcgcca ttggcgcgtg ctcaacaata tcagcttcca
     5881 ttgggggcgc tgctggccgc tcgacatcgc cctctgggac ttggccggca agatcgccgg
     5941 gcagccctgc tggaagctga tcggcggcct ttccgaccgc gtcaaagcct atgcgtcgtc
     6001 ggggacgctg cgcggcccgg ccgagctcgc cgacgccgcc gaggcctatc tcgccatggg
     6061 cttttcggcc ctgaagatcc gcttccatcg cggcgattgg cgcgccgaca tcaaggccct
     6121 cgaagcggtt cgcggccgaa tcggcgaccg gctcgacttg atggtcgact gcaatcaagg
     6181 ctggcggatg tcttgggaca cttacgcgcc ctggtcgctg aaggacgccg tcaaggtcgc
     6241 gcgggaattg gagcggctcg gcgtctattg gatggaggag ccgctgcatc gcggcgaccg
     6301 cgacggcatg cgtcgccttc gcgacatgac cgacattcgg atcgcgggcg gcgagatgac
     6361 gcgcgagctc tacgaattcc gcgacctgat cgccgaaggc tgcctcgacg tgctgcagcc
     6421 ggacgccgct ttggtcggcg gcctgaccgg cctgcgccgc gtcgccctga tggcgcagga
     6481 gcacaatctc gtcttcacgc cgcatacttg gaccaatgga atcggcgtcg tcgccaacgc
     6541 ccatctcacc gccggcctcg ccgacgcgcc tttcgtcgaa tatccctacg atccgcccga
     6601 atgggatgtg gatcgacgcg attacatgat gcgcgaaccc ttgaagatca gcgcggacgg
     6661 aaatatcgtg ctgaatgatc ggcccggcat gggctacgac cttgacgaag aaaggctcgc
     6721 gaaaacgcgc atcggataat tttagttggt tccgtccaaa cgcgcgtcgt ctcgaggggg
     6781 cgacgatgga tcgcaaatgg ctgccgctta acgcgctgcg cgctttcgag gccgcaggcc
     6841 agcatctgag ctttacggcg gccgcaaaca gcctgacggt ggcgcagagc gcggtcagcc
     6901 gccatgtcat cgttctggag aattttctcg gcgtcaccct gttcgaacgt cggccgcagc
     6961 aattggtgct gaccgaggcc ggccggcata tcctgcctgt tctgacgaag tctttcgacc
     7021 gcatcgacca ggcgctgagc gaagtcatca aggaaaaggg ccggccgaag cgggtgctga
     7081 aagtcgcgct gccgacgacg ttcgcccacc gcctcgccat ccctatcctg cgcgatttcc
     7141 gcgccgagca ccctggcatc acgctggaga tcgtctccaa gccgaccagc ggcgccgaag
     7201 tcgacggcga tgtcgacatc gccatcgtct attccgagcc gcgcgtcacc gacgcgatcc
     7261 tcgaccttct ctggatggtg cgctcgacaa tcctgtgcag cccgaaactg ctgcaaggcg
     7321 tcgagcgcat cgaccctgcc cggctgatcg cctcctgcga tcttctgcat gtaaaggtcg
     7381 acgaccggcc gcgccatttc ttctgggaga tgctgacgcg ctcgctcggc cgcccggatc
     7441 tgccggtcga tcgcggtctc gtcttcgaca cggcgcagct cgcggtgcaa tacgctctgt
     7501 cgggcgacgg cctggtgctc gccgatccga tgctgttcca ggaggaggtg cgatccgggc
     7561 ggctggtcag gcctttcgat ctcgggctcg aagaaggcta cggctattac ctgaccaccc
     7621 atccggaaga cctcaacaac gaggcggtgg cgctgttccg ctcctggctc atcgctcgct
     7681 tctcggccga gaccgcggaa gccgccgccg cgccgacggc cccggcgacc ccgcccgccg
     7741 cggacatgcc ggaagccgag cggccgaagc tgcgcgcggc gaagtagctc ctgcgatctt
     7801 cgcgcctgct tcgtgtcctt tgtgttggaa ttttaaaacc acgaaggacg tgaagggggc
     7861 gcgaaggtca taattggcga attatgcagg ttggccgcat caacatcgac gagagcgaga
     7921 tcagcgagac cttcgttcgc gcctccggcc cgggcggaca gaacgtcaat aaggtctcga
     7981 ccgcggtcga gctgcgcttc gacgcgcgag gctcgaagag cctgccggac gacgtttcgg
     8041 tccggctgca gcgcctcgcc gggtcgcgcc tgacgcagga cggaatcgtc gtcattttcg
     8101 cgcagcgctt ccgcagccag gagcgcaacc gcgccgatgc gcgcgaaagg ctcgcggcgc
     8161 tgctgatgca ggcgcagaag cggcccaccg tcaggcgtcc gacccgcccg accaaggcct
     8221 ccaaagagaa gcgcttagag tcgaagagcc tgcgcggacg gatcaagaat ttgcgccgcg
     8281 gtccgctcga cgagtaggcg ccgatttgga tcggccgttc gctcaggtct gatcgccagg
     8341 tcggcggcag gacgccgccg agcatgccgc tctttccgcc tggatgggcg ggcatggcgc
     8401 cgagccgtag gaacagaaga cgcagcaatc ccctggcttc ggcttgagca aagccccgca
     8461 gcttttgcat tcgtagaaaa cgcggcaagc gtcggtcggc atcgtctcga gcgcctgatg
     8521 acggcaaacc ggacaagtaa ccactgagtt cagttggatc atgacaatgc gccagattcg
     8581 gcgtgaggcg cccggctcgc ggctaggatc gagcctccag cctcggcttc atcgccttga
     8641 acgcggcctc catctgcccg tcgatatagg cggcgaaatc gtcgggcagc aggtcgacaa
     8701 tccagacgag ccgcgagcga cccgcgcctt cggcgaagac ccgcaaggcg gcgttgtaat
     8761 gggccatgcg gccgccgagg atcgcataga ccaagcgccg cgattcgtcg tcgcgcgaga
     8821 ccagcgtttc aatcgcctcg acgccattgg cgaatttgac gaagcgccgg tcgccgtcga
     8881 gccgcgatga gacgacgaag cccggcgcga cgcgcgtgtc gacggcgccg aaatcggcga
     8941 gagccgccca aacgtcggcc gcatcggcgt caagctgaag ctccttgcgg atcgacgcca
     9001 tcgccttacc gcgtcggcat cggatgctcg ccgcgatagt cgtagaagcc gcgctgcgtc
     9061 ttgcggccga gccagccggc ctcgacatat tttacgagca gcgggcaggg ccggtatttc
     9121 gaatcggcaa ggccttcgtg cagcacctgc atcacggcca ggcacgtatc gaggccgatg
     9181 aaatcggcga gctgcagcgg ccccatcgga tggttggcgc cgagccgcat cgccgtgtcg
     9241 atcgcctcga cggtgccgac gccttcgtaa agcgtgtaga tcgcctcgtt gatcatcggc
     9301 agcaggatgc gattgacgat gaaggccgga aaatcttccg acaatgtcgc cgtcttgccg
     9361 agcctgctga cgaacgcctt ggccgtctcg aatgtctcgt tctgcgtagc gatgccgcgg
     9421 atcagctcga ccaattgcat gcgcggcacg ggattcatga aatgaatgcc gatgaagcgc
     9481 tcgggattgg cggtgatcga ggcgaggcgg gtgatcgaga tcgaggacgt gttcgaggcg
     9541 acgatcgcat cgcttttgag gtaggggcgc aaattggtga aaatcttgcg cttgacctcc
     9601 tcgttctcgg aggccgcctc gatgacgaga tcgcaatcgg aaagcttgtc gagatcggtg
     9661 gtcgtatcga tgcgcgacag ggcctcgtgg cgcttggcct cgtcgatttg gccgcgggcg
     9721 acctcgcgcg cgaggtggcc gttgatcgtc gccagcgccg ctttgagtcg atcttcggtc
     9781 acgtcgctca tgacgacgtt gaggccggcg accgcgcaga cctgcgcgat gccgttgccc
     9841 atctgcccgg cgccgatcac gccaatcttg ttgatcgcca tcgtcatctc atcaatccga
     9901 ggggcggaag ctttgcgaga ttaggccgca aaggcggcgc gcccagggcc tcagcagcta
     9961 tcgtccggtc ttgcttaact cctcggcgag ttcgggcaag gcggtgaaga ggtcggcgac
    10021 gaggccgtaa tcggcgacct ggaagatcgg cgcctcctcg tccttgttga tcgcgacgat
    10081 aaccttcgaa tctttcatgc cggcgagatg ctggatcgcg ccggaaatgc cgaccgcgat
    10141 atagaggtcc ggcgccacta ctttgccggt ctggccgacc tgccaatcgt tcggcgcgta
    10201 gccggcgtcg acggcggcgc gcgaagcgcc catcgccgcg ccgagcttgt cggccaccgg
    10261 ctcgatatat ttcttgaagt tctcggcgtt ctgcatcgct cttccgccgg aaatgacgat
    10321 tttcgccgag gtcagctccg gccggtcgga cttggccagc gcctcgcctt tgaacacgga
    10381 gagcccggga tccggcgcgg cggcgaccgg ttcgaccggc gccggaccgc cggcgcccgt
    10441 cgcctggaac gaggcggtgc ggaccgtgac gatctttttc ggctcactcg tttgaaccgt
    10501 ctggatcgca ttgccggcat agatcggccg ctcgaacgta tcgggcgaaa cgactttgat
    10561 gatgtcggag acctgcatca cgtcgagcaa ggcggcgacc cgcggcatca cattcttggc
    10621 gtaggcgctc gatccagcga cgatcgccga atagtcgccg gccagcgaga cgaccagcgc
    10681 ggcgagcggc tcggcgagac cgtgctcgta gcgcggatcg tcggcgacgc gcactttgcg
    10741 caggccggcg agcttcgacg ccgcctccgc ggccgcgccg acattgcggc cggcgacgag
    10801 aatgtcgacc ggctcgccga gctgcgcggc ggcggtgagc gccttggcga cgatgtcggc
    10861 gagatggccg ttggcgactt ccgccaggag gagggtcgcc atgtcagatc actcccgctt
    10921 cgtctttgag cttggcgacg agctccgcca ccgatttgac cttgacgccg gccttgcgtc
    10981 cgcccggctc gaccgtcttc agcactttga ggcgcggcgc gacatcgacg ccgaaatccg
    11041 ccggcgcctt ctcctcgatc ggcttcttct tggccttcat gatgttcggc agcgaggcgt
    11101 agcgcggctc gttgaggcgc agatcggtgg tgacgatcgc cggcagcttg agctcgaccg
    11161 tctgcaagcc gccgtcgact tcgcgcgtca cggcgacggc gccctcgccg atttcgacct
    11221 tcgaggcgaa ggagccctgg ccccagccga gcagggcggc gagcatctgg ccggtctgat
    11281 tgcaatcgtc gtcgatcgcc tgcttgccga gaatgaccag gccgggctgt tccgcctcga
    11341 ccacgccctt gagcaatttc gccacggcca aaggctcgac gatctggtcg gtcttgatga
    11401 gcacgccgcg atcggcgccc atggcgaggg cggtgcggat ggtttccgcg gcttgggccg
    11461 cgccgatcga gacgatgacg acctcgctgg ctttgcccgc ctccttttgg cgcagcgcct
    11521 cttcgaccgc aatctcgtcg aacggattca tcgacatttt gacgttggcg agctcgacgc
    11581 ccgttccgtc cgccttcacc ctgatcttga tgttgtaatc gaccacccgt ttgaccggca
    11641 cgagaatctt catcgcttcg cgctctcctt ctcgccgcgg gaaatcgcgt ttcgcctcgc
    11701 cgagaatgcc ttgagacccg ttccggccgc cgccctcaac cttcaggcgg cgccgcgccc
    11761 gctcggcggg ccgccagcgc gcggcgccgg atgtgggcaa cccctaatgg ccgccaaagg
    11821 gcgagtcaac gcggcgcgcg ggctgcccgg ctgggccgct tcggctgatt tgcgagtcgg
    11881 aaagcgccgg cgcgattgac tttccgcgcg gcgaaaaccg attgtgcgac gatggtcttc
    11941 ctttcacagc gcggcgctaa agcctgagcc ggcgatgaac gaactgaatt gtcagcgcga
    12001 agaaccgcgg atcggcgcgc cgtcgcgccg cgaatttctt ggcggcgcgg cggcgctcgg
    12061 ctgctccggt tatgcggcgt cgagccgtga cgcggcggcc gccgagacga tcaagaaagg
    12121 cggcctgttg cggcttggcc ttgccggcgg cggcccgtcg gacagtttcg atccggcgag
    12181 ctacgttgac gcggtgatga tcgtcgccgg ccgcggcctg ttcaacggcc tcgtcgaatg
    12241 gaccgcggaa ggcggcgtca agccggagct cgccgccaat tgggagccga gggacggcgc
    12301 caagacctgg attttcaatc tccgcaaagg gatcaaattc tccaacggcc aggaattcac
    12361 cgccgaggac gcggtcgcct cgctcaatcg cggtcgcgtc ggcaaatcgc tcgcgtcccg
    12421 cgattcgacg ccgatcgccg agatcaagaa ggccgacaaa taccaggtcc aagtcaccct
    12481 ggagactccc gacgcggact ttccctatgt gcttaccgat tgccgcctgc tgatggcccc
    12541 ggcgggtttc aaggactggt cgaagccgat cggcaccggc gcctacaggc tcgacaagtt
    12601 cgagccgggc gtgcgcgtgt cgctgaagcg ggcgccggat tattggcgcg ccggcgaggg
    12661 ccgcggcaat gccgaggccg ccgagatcac tgtgctcaac gaactttccg agcgcgtcaa
    12721 tgcgttggtc gccggtcata tcgacgtcat caatcgcgtc gatccgagga ccgtcgcgct
    12781 gttgcagaag acgccgaaga tcgaaaccct tttcgcggcg agcggctggc gtccggtcat
    12841 ggccatggcg gtcgatcagc cgcccttcaa caatgccgat ttccgcctcg cgatgaaata
    12901 cgcgatcgat cgcgagcaga tgatccgcgc gctctatgcc ggctacgccg cgctcggcaa
    12961 cgatcatccg attccgccga acgatcccta tttcaacaag gagctgccgc agcgaaagca
    13021 cgatccggat cgggcggcgt tctatctgaa gcggtccggg ctgagcggcg ccgagattgc
    13081 gctcgaagtc tcgcccgccg ccttcgccgg cgcgccggag gccgcctccc tgctgcaggc
    13141 gagcgccaag caggcgggcg tcaggatgca gatcaagagc gaaccggcag aagggttctg
    13201 gcgcaatgtc tggctcaaag cgcccttcgt cgaaagctat tggcgcggcc ggccggccgc
    13261 cgtccatatg ctcgccatcg ccttcggcgc ggactcgcca ttgaacgaga cgcattggag
    13321 gagcgacaaa ttcgaaaagc ttttggccga cgcgagaagc gagaccgaag aggccaagcg
    13381 caagccctat atctgggaaa tgcaagccat gcttcacgac gagggcggcg cgatcgttcc
    13441 gctattccgc gactggatcg acgcgcggcg cgacgcggtc ggcggttatg cggcgcgagg
    13501 cggcgcggat ctggccaacg gctatattct cgaaaaagcg tttctgaagg cctgatccgg
    13561 cttgctcgcc agccgcgccg ttcgcaacgc tgcggatcgc ctcgcctggg cgcacgccgc
    13621 tcccgccgct tgcccggcgc gaggctcatt ggctatacgg ctcttcgccg gacccccaat
    13681 attgagtccc tcggagggcg aggccaactg ggctgccgaa cgccggccgt ggcgtcgccg
    13741 atcggtccca gggctttgcg tctttatatg cgctggaagg cgccagccgt ttcaagcgcc
    13801 gagggtcgct gcgcgtcagc caaagcccga aggtggcgcg gcggcggtta ataaagttgt
    13861 tgacagttcc gcctgagcgc gaggccctgt tggcgcgacg gcggagcaga acgaggtaga
    13921 atcatgcgcg ccccgggcgg accgcgcctt ttgctgcggc ggcttcgcga ggtgatggcg
    13981 gagcccgtca cggctcaggc gcggctcgac agaatcgtcg tcctcatcgc ctccaatatg
    14041 gtggccgagg tctgctcgct ctacgtcgcg cgggccgacg gtcccttgga gctctttgcg
    14101 accgagggat tgaagcgcga agcggtgcat gtgacgacca tgcgccccgg cgagggcctg
    14161 gtcggcctga tcgcctcgac ggccgagccg ctcgcgcttt ccgacgcgca gagccatccg
    14221 gccttctcct acaagccgga gacgggcgaa gaaatctacc aatccttcct cggcgtgccg
    14281 gtcctgcggg gcggcgccac gctcggcgtc ttggtcgtcc agaaccgcgc ccggcgcacc
    14341 tattccgaag aagaggtcga ggcgctgcaa acgacggcga tgctgctcgc cgaactgatc
    14401 gcctcgggcg aattgcagtc gatcatccgg ccgggcgtcg acatcgcgct gcgccggccg
    14461 gtcgcgctca aaggcgcgcc gatcgcggag ggcctcggcc tcggccatgt cgtcctgcac
    14521 cagccgcggg tgacggtctc caagctcgtc gccgacgaca ttcccgccga gatcgagcgc
    14581 ctcgaccggg cgatcgaagc gatgcgcgcc tcggtcgacg aattgatcgg ccgcaccgaa
    14641 agcgccggcg acggcgagca ccgcgacgtg ctcgaagcct tccgcatgat cgcccatgac
    14701 cgcggctggc tgcgccgcct caacgaagcg gtgatgagcg gcctgaccgc cgaagccgcg
    14761 gtcgagcggg tgatgaacga cgcgcgcgcc cggcttcagc gccagaccga tccctatttg
    14821 cgcgaccggc tgcacgacct ctccgatctc gccaatcgcc tgctccatca actggctggc
    14881 cagagcctga tcctcgatcc gagcgaattg ccggaaaacg ccattctcgt cgcccgctcg
    14941 atgggaccgg cggccttgct cgactacgat cgccagcgcc tccgcggtct cgtgctggag
    15001 gaggcgggcg cggccagcca cgtcgcgatc gtcgcccgcg ctttgtcgat tcccgcggtc
    15061 agcgaggtcg ccaacatctc ggaaatcgcc gagcagggcg acgcgatcat tgtcgacggc
    15121 ggcgtcggcg aagtgcaatt gcgcccgtcg gccgatgtcg aggcctccta tgccgagaag
    15181 gcccgcctgc gcgcccgccg gcaggagcaa tatcgcgaat tgcgcgacac gccggcgatc
    15241 acccgcgacg gcgtcgcgat cgatctgcat atgaacgccg gcctgatcgt cgatttgccg
    15301 catctcgacg agagcggggc gcaggcgatc ggcctcttcc gcaccgagtt gcaattcatg
    15361 ctggccgcgc gctttccgcg gctcgccgat cagcagcagc tctatgagac cgtgttcaac
    15421 gcggccggcg agaggcctgt gaccttccgt acgctcgata tcggcggcga caaggttctg
    15481 ccctatatga aagggccgga ggaggaaaat ccggcgctcg gctggcgcgc gatccgcatc
    15541 gggctcgaca agcccgcttt gctgcgcacg caattgcggg cgatgctgcg cgccggcgcg
    15601 gaccgcaatt tgcgcatcat gctgccgatg gtggcgagcg tcgatgaatt cgtcgccgcg
    15661 cgcgccttgc tcgagcgcga actcgcgttc aacgaccgct gcggccggcg ccccccggcc
    15721 gacatcaagc tcggcgtgat ggtcgaggtt ccgtccctgc tctggcagct cgacgagatc
    15781 gccgagcgag ccgatttcct ctcggtcggc tccaacgact tgatgcaata tctctacgcc
    15841 gccgatcgcg acaaccgccg ggtggcggat cggttcgatc ccctctccgc cggtttcctg
    15901 cgcgcgctgg cggcgatcgc cgaagccggg cgcaacgccg gcaagccggt gactttatgc
    15961 ggcgagatcg gcggccgcac tttggaggcg atgacgctga tcgcgatcgg ctatcgcggc
    16021 ctctcgatgt cggcggcctc gatcggcccg gtcaaggcga tggtcctgtc gctcgacgcc
    16081 gcggcggcga cggaagcgac cgcggagctg ctggcccgca gcggcgaggc ggcgtcgttg
    16141 cgcgagccgc tgaccgcgct ggcggagcgg ctgggggtga ggctgtagcc cggccgtcag
    16201 tgcgagaagc gaagcgacga agcaagcctt gcacaaggcg cagcgtcagg agcaaaaggg
    16261 aagaaagaaa cgacgtggag gcggaatact tcaaactcga ccagcgtcgc ggcgccacgc
    16321 cgtccgatcg ccgtcaaccc gccatagcgc ctcgaaggct cgtgtgagac gacgccgccc
    16381 gccttcgagg cgctatggcg ggtcctccgc ttcgcttcgg cccttcgggt gactgcgatc
    16441 cgccggcgcg gcttattggc tatcaagacg ttttgctgac gaagccggcc gacggccggt
    16501 tcgatcagca aaacgtcttg atagcaaaac cgccatcgcc gagcg
//
LOCUS       DATGJW010000051         3106 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1047016, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000051 DATGJW010000000
VERSION     DATGJW010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3106)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3106)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3106
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1047016"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1017
                     /locus_tag="VKU03_01485"
     CDS             <1..1017
                     /locus_tag="VKU03_01485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002971229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenine deaminase C-terminal domain-containing
                     protein"
                     /protein_id="HLJ69957.1"
                     /translation="EKLSKGMFILVREGSVSKDLHALAPLITERHSPYIAFCTDDRNP
                     LDIAEEGHLDFLIRTAIKLGAPPLAAYRAASISAARIFGLMDRGLVAPGWRADLAVID
                     DLESCAVSQVFTAGRPVEETLFGQRKIIAPVGRGSVKAARVTAADFVARGQSPSTQAI
                     GVVPGKIITERAPVSLPFVSGARKIDLDQDAVKVAVVGRHGVNRNIAVAFVKGFGMKR
                     GAIASSVGHDCHNICVVGANEADMAVAVNRLIEIEGGFAVAEGGKILAEIALPIAGLI
                     SLMSFEEVRKALIPLRAAAKSLGVALPEPFLQVAFLPLPVIPHLKITDKGLVDVDRFE
                     FVRD"
     gene            complement(1081..1464)
                     /locus_tag="VKU03_01490"
     CDS             complement(1081..1464)
                     /locus_tag="VKU03_01490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF423 domain-containing protein"
                     /protein_id="HLJ69958.1"
                     /translation="MARPPFALAALASLMGAAGVGLAAAAAHGRGADGLARIAALFLI
                     LHAAALLGLAALAVQFAGGRLRRALLACSFGLGCAAILFSADLAARALAGGRLFPMAA
                     PIGGTGMILFWLALAAIFVFRAIGD"
     gene            1599..2243
                     /locus_tag="VKU03_01495"
     CDS             1599..2243
                     /locus_tag="VKU03_01495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DedA family protein"
                     /protein_id="HLJ69959.1"
                     /translation="MPLVGHLHGVVQHYGYFAVFATVCLECLGVPIPGETALVAAAIF
                     AAAGEINIFGVIGAAALAAIIGGSIGYWIGRALGFPLVVRYGRYIHLDEPRLKLGQYL
                     FKRHGGKIVFFGRFIALLRAFAALLAGINRLPWPRFSLFNALGAVAWASVFGCGGYFL
                     GQAFEHYARPVGIAVLVCAAIGVVAASRFIAHHERRLIEEAEAAMPGPLVAPRD"
     gene            complement(2313..>3106)
                     /locus_tag="VKU03_01500"
     CDS             complement(2313..>3106)
                     /locus_tag="VKU03_01500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176819.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLJ69960.1"
                     /translation="QGARGAEIATLEGRQLYARVLGDDPDTDLALLRIDDGATLPAAK
                     LGDSAKLKRGQIAIAIGNPLGFDASVTAGVVSALGRSLRAKSGRMIEDVIQTDAALNP
                     GNSGGPLVSSRGEVIGINTAIIMGAQGICFAVAANTANFVLGEIARHGRVRRAYIGIG
                     AGTVAIPRRIALRLGLEQANGARVLQVEQGSPADQAGLLSGDLVVALDGKRVNGVADL
                     LRLLDADKISRTVSIDVIRRSEPLRFWIGPTERVGAQLAASLRSQ"
BASE COUNT          487 a         1070 c         1045 g          504 t
ORIGIN      
        1 gagaagctct ccaagggcat gttcatcctg gtgcgcgagg gctcggtctc gaaagacctt
       61 cacgcgctcg cgccgctgat caccgagcgc cactcgccct acatcgcctt ctgcaccgac
      121 gaccgcaatc cgctcgacat cgccgaggaa ggccatctcg atttcctcat ccgcaccgcc
      181 atcaagctcg gcgcgccgcc cctcgcggcg taccgcgccg ccagcatttc cgcggctcgg
      241 attttcggcc tgatggatcg cggcctggtc gcgccgggct ggcgggccga tctcgccgtc
      301 atcgacgatc ttgaatcttg cgcggtctcg caggtcttca ccgccggacg gccggtcgag
      361 gaaacactgt tcggacaaag aaagatcatc gcgccggtcg gccgcggcag cgtcaaggcg
      421 gcgcgcgtca cggcggcgga tttcgtcgcc aggggtcaaa gcccctcgac ccaagcgatc
      481 ggcgtcgttc ccggcaagat catcaccgag cgcgcgccgg tgtcgctgcc tttcgtaagt
      541 ggcgctcgca agatcgacct cgatcaggac gccgtcaaag tcgcggtggt cggccgccac
      601 ggcgtcaacc gcaatatcgc ggtcgccttc gtcaaaggat tcggcatgaa gcgcggcgcg
      661 atcgcctcgt cggtcggcca tgattgccac aatatctgcg tcgtcggcgc gaacgaggcg
      721 gacatggcgg tcgcggtcaa ccggctgatc gagatcgaag gcggcttcgc cgtggcggaa
      781 ggcggcaaga ttctcgccga aatcgcgctg ccgatcgccg gcctgatcag cctgatgagc
      841 ttcgaggagg tgcgcaaggc gctgatcccg ctgcgcgcgg cggcgaaaag cctcggcgtg
      901 gcgctgcccg aacccttcct gcaagtcgcc ttcctgccgc tgccggtgat cccgcatctg
      961 aagatcaccg acaaggggct ggtcgatgtc gaccggttcg aattcgtgcg ggattgatcg
     1021 cgcacgtacc cgtcattgct tcgctccttg caatgacggc gcggtacggg gcgccgcggc
     1081 tcaatccccg atcgccctga agacgaagat cgccgcgagc gccagccaga acaggatcat
     1141 ccccgtcccg ccgatcggcg ccgccatcgg gaaaagccgg ccgccggcca gggcgcgcgc
     1201 cgcgagatcg gcgctgaaca gaatcgcggc gcagccgagg ccgaacgagc acgccagcag
     1261 cgcgcggcga aggcggccgc ccgcgaattg aacggcgaga gccgccaggc cgagcagcgc
     1321 cgcggcgtgc aggatcagga acagcgcggc gatgcgggcg aggccgtccg cgccgcggcc
     1381 atgcgcggcg gccgcggcga ggccgacccc cgccgcgccc atgagcgagg cgagcgcggc
     1441 aagggcgaag ggaggacgcg ccatgaatac ttgttacccg agccggcgcg cgaagggcag
     1501 acgcgccggc tttcttttca agcggccgct tctcgcgccc atcgacctgc gttaagggag
     1561 gcctccccgc ggctcaggcc gcctggcagg gtcagccgat gcctcttgtg ggccatctcc
     1621 acggcgttgt tcagcattac ggctatttcg ccgtgttcgc gacggtctgt ctcgaatgtc
     1681 tcggcgtgcc gatccccggc gagacggctt tggtggcggc ggcgattttc gccgccgccg
     1741 gcgaaatcaa catttttggc gtgatcggcg cggccgccct ggccgccatc atcggcggct
     1801 cgatcggcta ttggatcggc cgcgcgctgg gctttccgct ggtcgtccga tatggccgct
     1861 atatccatct cgacgagccg cggctgaagc tcggccaata tctgttcaag cggcatggcg
     1921 gcaagatcgt cttcttcggc cgcttcatcg ccttgctgcg cgccttcgcc gccttgctcg
     1981 cgggaatcaa ccgcctgccc tggccgcgct tctcgctgtt caacgcgctc ggcgcggtgg
     2041 cctgggcgag cgtgttcggc tgcggcggct atttcctcgg ccaggccttc gagcattatg
     2101 cgcggccggt cgggatcgcg gtcctggtct gcgcggccat tggcgtggtg gcggcgagcc
     2161 gcttcatcgc ccatcacgag aggcggctga tcgaagaggc ggaggcggcg atgccggggc
     2221 cgcttgtcgc gccgcgagat tagcggccta tccagaactt ccccacagct tgtccccagc
     2281 ctgtcagcca catttccaca agcctcgtac tgtcattgcg agcgaagcga agccgccagc
     2341 tgcgcgccga cgcgctccgt cggaccgatc cagaagcgca atggctccga ccgccggatc
     2401 acatcgatcg agaccgtgcg gctgatcttg tcggcgtcga gcaggcgcag caagtcggcg
     2461 acgccgttca cgcgcttgcc gtcaagcgcg accaccaaat cgccgctgag caagccggcc
     2521 tggtcggccg ggctgccctg ctcgacctgc aatacgcgcg cgccgttggc ctgctccaag
     2581 cccaaacgca gcgcaatgcg ccgcggaatg gcgaccgtgc cggcgccaat gccgatatag
     2641 gcgcggcgga cgcggccatg ccgcgcgatc tcgccaagca cgaaattggc ggtgttggcg
     2701 gcgacggcga agcagatgcc ctgcgcgccc atgatgatcg ccgtgttgat cccgatcacc
     2761 tcgccgcgcg aggagacgag cgggccgccg gaattaccgg gattgagcgc ggcgtcggtc
     2821 tgaatcacgt cctcgatcat ccgcccgctt ttcgcgcgca acgaacggcc gagcgccgag
     2881 acgacgccgg cggtgacgct ggcgtcgaag ccaagcgggt tgccgatcgc gatggcgatc
     2941 tggccgcgct tgagcttggc ggaatcgccg agcttagccg ccggcaaggt cgcgccgtcg
     3001 tcgatgcgca gaagcgccag atcggtgtcc ggatcgtcgc cgaggacgcg ggcatagagc
     3061 tggcggcctt ccagcgttgc gatttccgcg ccgcgcgcgc cttgca
//
LOCUS       DATGJW010000052         6092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_113202, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000052 DATGJW010000000
VERSION     DATGJW010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6092
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113202"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..620)
                     /locus_tag="VKU03_01505"
     CDS             complement(<1..620)
                     /locus_tag="VKU03_01505"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLJ69961.1"
                     /translation="MPRVLLVGDVMVDMIVRPEGPIAVGSDRRATIRARPGGSAANQA
                     AWLAHFGVEARLVGRVGAADRDAQVALFAKAGVEAHLAADESRETGRLIALIDPSGER
                     SFLTDRGANDGLTASDIPDALFDGADHLHVSGYSFVHEGPRAAMIDLIARAKARRVPA
                     SVDPASSEFLREIGPQNFLAWTAGVEMCFPNEDEARMLTGAERTDE"
     gene            complement(621..1544)
                     /locus_tag="VKU03_01510"
     CDS             complement(621..1544)
                     /locus_tag="VKU03_01510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HLJ69962.1"
                     /translation="MTIARLLDIGADVAAAIEARRPVVALESTIITHGMPFPQNFETA
                     SKLEETVRAQGATPATIAVVDGRLVVGLDKAGLEKLARLTGGVAKASRRDLAAVLARK
                     GAAGTTVAATMYVAALAGIEIFATGGIGGVHRGAEETFDISADLVELSRSKVAVVCAG
                     AKSILDIPKTLEYLESHGVPVIGYRCDEFPAFYARSSGVKLDHRCEGLHDLACMIRLQ
                     REIGPGGLLIANPIPEAHALDASQMEARIEEAVREAKAKGIGQKEVTPFLLRRLVEMT
                     GGRSLAANMALVEANAALAGQVAVELARLTS"
     gene            1741..2391
                     /locus_tag="VKU03_01515"
     CDS             1741..2391
                     /locus_tag="VKU03_01515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69963.1"
                     /translation="MDEKKPDHLKSVPPSLKAALRRARVESAEQSHVVAELRETETAR
                     LEILEEAIRPILDQVPAEVDIFDPGLSHGERPRLFIDMIGFVEMAHDRRVYRFLQDTR
                     HGRALIAESDRVERMVEAIADYIARRLVERERALASDWRSRDQAEAAPAERGEAKQAA
                     ANPGLAERGGLAEFFRIFFMMLGAAAFCAVVALGLAMLWQIYGRALWSAYIGPPLF"
     gene            complement(2417..3319)
                     /locus_tag="VKU03_01520"
     CDS             complement(2417..3319)
                     /locus_tag="VKU03_01520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HLJ69964.1"
                     /translation="MTFTIAHLSDAHIGPLPRPTLRQLAGKRGLGYVNWRRARHREHD
                     MDLLGRLVADLSAQKPDHVAMTGDILNIGLPAEFPIAAAWLSTLGSPRDVSFTPGNHD
                     AYTRDAMRHLDGTFAPWVAGDAPGGPIFPYLRIRGDVALIGLSSGLPTGPLMATGRLG
                     AGQLSRLETLLREIGEKNLARVVLIHHPPLRAGASPMRRLVDADALEAALVRTGAELV
                     LHGHNHVGSIAHLKSSASRAAGGRIPIIGVSSASSVSRAPRHRAAYHLLRLERAGGVW
                     RTFGHARGPLDGSREVGELREISP"
     gene            complement(3319..4290)
                     /locus_tag="VKU03_01525"
     CDS             complement(3319..4290)
                     /locus_tag="VKU03_01525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009027621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin family protein"
                     /protein_id="HLJ69965.1"
                     /translation="MKAFADLTEREILAIGVSAEEEDARIYQAFAEDLAVRYPDSAKI
                     FEEMAEEEKTHRAKLLSMYEERFGQNLPPIRREDVKGFLKRRPIWLTKNLPLETIRKQ
                     AETMEFQAARFYSRAAEQATDVGVRRLLGELAIIEQGHEDLAHKLAKENLTADARAKE
                     DETARRIFVLQYVQPGLAGLMDGSVSTLAPLFAAAFATHNNWSTFLVGLAAAIGAGIS
                     MAFAEALSDDGSLTGRGAPLIRGGVCGVMTAIGGLGHAIPYLVPDSWPNAFWVATSIA
                     CVVVFFELWAIAYIRARFMDTPFLQAAFQVVIGGVLVLIAGMLIGGA"
     gene            4369..4848
                     /locus_tag="VKU03_01530"
     CDS             4369..4848
                     /locus_tag="VKU03_01530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLJ69966.1"
                     /translation="MTDQPKSLFARLRSRFFHLYFVATRPMTLGVRAAVFDSQGRVFL
                     VRHTYVPGWHFPGGGVEPGETTLDALARELLEEGAISVEEEPRLHGLFFNRGASRRDH
                     VALFIVRRFRQDAPPKPNREIAATGFFAPDALPEATSRPTQARLKEILQAAPPSPLW"
     gene            5076..5240
                     /locus_tag="VKU03_01535"
     CDS             5076..5240
                     /locus_tag="VKU03_01535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HLJ69967.1"
                     /translation="MSKALKKFIREESGATAIEYGLIASLIALVIIGAITVVGTKLQT
                     TFNEVASNLH"
     gene            5554..6051
                     /locus_tag="VKU03_01540"
     CDS             5554..6051
                     /locus_tag="VKU03_01540"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HLJ69968.1"
                     /translation="MPDIPLVLEAETPADAEAVERLSARVFGPGRFARTAYRLRETTP
                     HDLNLSFIARVGSLLVGANRMTPIVIGETPALLLGPLIVEPVFRSQGIGEALVTRSLD
                     AARQAGAKLSILVGDEPYYSRMGFKAAPPGRLQMPGPVDPARLLYCEIAPGAFEGVQG
                     RVRGA"
BASE COUNT          988 a         2067 c         2065 g          972 t
ORIGIN      
        1 tgttcgtccg tacgctcggc gccggtcagc atgcgcgctt cgtcctcgtt cggaaagcac
       61 atctcgacgc ccgccgtcca ggcgaggaag ttctgcggcc cgatctcgcg caggaactcg
      121 ctcgacgccg gatcgaccga ggccgggacg cggcgcgcct tggcgcgggc gatgaggtcg
      181 atcatcgccg cgcgcgggcc ttcatgcacg aaggaatagc cggaaacatg cagatgatcg
      241 gcgccgtcga aaagcgcgtc ggggatgtcg cttgccgtga ggccgtcatt ggcgccgcga
      301 tcggtgagga acgagcgctc gccggaagga tcgatcagcg cgatcaggcg gccggtttcg
      361 cggctttcgt cggcggcgag atgcgcttcg accccagctt tggcgaagag cgcgacctgc
      421 gcgtcgcgat cggcggcgcc gacgcggcca acaagccgcg cctcgacgcc gaaatgagcg
      481 agccaggcgg cctggttggc cgccgagccg cccggccgcg cgcggatcgt cgcgcgccgg
      541 tccgagccga ccgcgatcgg cccttccggc cgcacgatca tgtcgaccat cacgtcgccg
      601 acgagaagaa cgcgcggcat tcaggaggtc agccgcgcca attcgaccgc gacctgcccg
      661 gccagcgccg cattggcctc gaccagcgcc atattggcgg cgaggctccg gccgccggtc
      721 atctcgacca ggcggcgcag caggaagggc gtcacctcct tctggccgat ccccttcgcc
      781 ttggcttcgc gcaccgcctc ttcgatccgc gcctccatct ggctcgcatc gagcgcatgg
      841 gcttccggga tcggattggc gatgagcagg ccgcccggcc cgatctcgcg ctgcaggcgg
      901 atcatgcagg caagatcgtg caggccttcg cagcgatggt cgagcttgac gccgctcgaa
      961 cgcgcgtaga aggccgggaa ctcgtcgcag cgatagccga tcaccggcac gccatgcgat
     1021 tcgagatatt cgagcgtctt gggaatatcg aggatggatt tggcgccggc gcagaccacc
     1081 gcgaccttgc tgcgcgacaa ttcgacgaga tcggcggaaa tgtcgaacgt ctcctcagcg
     1141 ccgcgatgga cgccgccaat gccgccggtg gcgaagattt cgattcccgc caaggccgcg
     1201 acatacatcg tcgcggcgac cgtggtgccg gccgcgccct tgcgggcgag caccgcggca
     1261 agatcgcgcc gcgacgcctt ggcgacgccg ccggtgagcc gggcgagctt ttcgaggccc
     1321 gctttgtcga ggccgacgac cagccgcccg tcgacgaccg cgatcgtcgc tggcgttgcg
     1381 ccttgcgcgc gcaccgtctc ttcgagcttc gacgccgtct cgaaattctg cggaaacggc
     1441 atgccatggg tgatgatggt cgattcgaga gccacgaccg gacggcgcgc ctcgatcgcc
     1501 gcggcgacgt ccgcgccgat atcgagcagg cgggcgatgg tcattggacg aggctccctg
     1561 gggcggcgcg cgattgaggc tttgcgtctt atcagcttga gcgccgcgaa ccaacgcggc
     1621 gggcgggcgg gacgttcccc ttctcgccgc ttgcggggag aaggggatgc gcgcattagc
     1681 gcggcgcggc ggcgcgtgct attgagcgcc gcgccaatcc tttttccgca aggggcggcc
     1741 atggacgaga aaaaacccga ccatctcaag agcgtgccgc cgagcctgaa ggcggcgctg
     1801 cgtcgcgccc gcgtggaaag cgccgagcaa agccacgtgg tcgcggaatt gcgcgagacc
     1861 gagacggcgc ggctggaaat tctcgaagag gcgatccgcc cgatcctcga tcaagtgccg
     1921 gccgaggtcg atattttcga ccccggcctc tcgcatggcg agcggccgcg cctgttcatc
     1981 gacatgatcg gctttgtcga gatggcgcat gaccggcggg tctaccgctt cctgcaggat
     2041 acgcggcatg gccgcgcgct gatcgccgag agcgaccgcg tcgagcgcat ggtcgaggcg
     2101 atcgccgatt acatcgcgcg gcggctggtc gagcgcgagc gcgcgctcgc ctccgattgg
     2161 cgcagccgcg atcaggcgga ggcggcgccc gcggagcgcg gcgaggccaa gcaggcggcg
     2221 gccaatcccg gcctcgcaga gcgcggcggc ctggcggagt tcttccgtat tttcttcatg
     2281 atgctcggcg cggcggcgtt ctgcgccgtc gtcgcgctcg gcctcgccat gctctggcaa
     2341 atctatggcc gcgccttgtg gagcgcctat atcgggccgc cgctgtttta gccggcgtcg
     2401 cttccagaaa cgtacgtcaa gggctgatct cccgcagttc gccgacctcg cgcgaaccgt
     2461 cgagcggccc gcgcgcatgg ccgaatgtcc gccagacgcc gccggcccgc tccagccgca
     2521 gcaggtgata ggcggcgcga tggcgcggcg cgcgcgaaac cgaggaggcc gaggagacgc
     2581 cgataatcgg aatgcgcccg ccggccgcgc gcgaggcgga cgatttgaga tgggcgatcg
     2641 aaccgacatg gttgtggcca tgcaggacca actcggcgcc ggtccggacg agagcggctt
     2701 cgagcgcgtc ggcgtcgacc agccggcgca tcggcgacgc gccggcgcgc aagggcggat
     2761 gatggatcag cacgacgcgc gcgagatttt tttcaccgat ttcccgcagc agagtttcga
     2821 ggcgggagag ttgcccggcc ccgagccggc cggtcgccat caacgggcca gtcggcaggc
     2881 cggacgacag gccgatcaac gcgacatcgc cgcgaatcct taaatacggg aagatcggcc
     2941 cgccgggcgc atcacccgca acccaaggcg cgaaagtccc gtcaagatgc cgcatcgcgt
     3001 cgcgcgtata ggcgtcatgg ttgcccggcg tgaaggagac gtcgcgcggc gatccgagcg
     3061 tcgaaagcca ggccgcggcg atgggaaact cggccggcag gccgatattg aggatgtcgc
     3121 cggtcatcgc gacatgatcg ggcttctgcg cgctgaggtc ggcgacgagg cggccgagca
     3181 gatccatgtc gtgctcgcga tggcgagcgc gccgccaatt gacatagccg aggccgcgct
     3241 tgccggcgag ctggcgcaag gtcgggcgcg gcagcggccc gatatgcgcg tccgagagat
     3301 gcgcgatggt gaaagtcatc acgccccgcc gatcaacatg ccggcgatga ggaccagcac
     3361 gccgccgatc acgacctgga aggccgcctg caggaacggc gtatccatga agcgcgcgcg
     3421 gatataggcg atcgcccaga gctcgaaaaa gaccacgacg caggcgatcg aggtcgcaac
     3481 ccagaaggcg ttcggccagg aatcgggcac gaggtagggt atggcgtggc cgaggccgcc
     3541 gatcgccgtc atcacgccgc agacgccgcc gcggatgagc ggcgcgccgc gccctgtcaa
     3601 cgagccgtcg tcggacaggg cttcggcgaa ggccatcgag atgccggcgc cgatcgccgc
     3661 ggcgaggccg accaggaacg tgctccaatt gttgtgcgtg gcgaaggccg cggcgaagag
     3721 cggcgccagg gtcgaaaccg agccgtccat caggccggcg aggcccggct gcacatattg
     3781 caggacgaag atccgccgcg ccgtctcgtc ctccttggcg cgggcgtcgg cggtcaggtt
     3841 ctccttggcc agcttgtggg cgaggtcctc atggccctgc tcgatgatgg cgagctcgcc
     3901 gagcaggcgg cgcacgccga catcggtcgc ctgctcggcc gccctcgaat agaagcgcgc
     3961 cgcctggaac tccatcgtct cagcctgctt gcggatggtc tccagcggca ggttcttggt
     4021 cagccagatc ggccggcgct tgaggaagcc cttgacgtcc tcgcgccgga tcggcggcag
     4081 attctggccg aagcgctcct catacatgga gaggagcttg gcgcgatggg tcttctcctc
     4141 ctccgccatt tcctcgaaaa tcttcgccga atcgggatag cgcacggcga ggtcctcggc
     4201 gaaagcctga tagatgcgcg catcctcctc ctcggcggaa acgccgatgg cgaggatttc
     4261 gcgctccgtg agatcggcga aagctttcat cgtcagcccc gaaacagatt ataatagttc
     4321 taaagtgagc cgcggcgaga ttcaaccctg ccgcgcccag gagagcccgt gaccgaccaa
     4381 ccgaaatcgc tgttcgcgag gctgcgctcg cgctttttcc acctctattt cgttgcgacg
     4441 cggccgatga cgctcggcgt ccgcgcggcg gtcttcgatt ctcaagggcg cgtctttctg
     4501 gtgcgccaca cctatgtgcc cggctggcat tttcccggcg gcggcgtcga acccggcgaa
     4561 accacgctcg acgccctggc gcgcgaactc ttggaggaag gggcgatctc ggtcgaggaa
     4621 gaaccgcgcc tgcacggatt gttcttcaat cgcggcgcgt cgcgccgcga tcacgtcgcc
     4681 ctgttcattg ttcggcgctt ccgccaggac gcgccgccca agcccaatcg cgagatcgcg
     4741 gcgaccggat tcttcgcccc ggacgccttg cccgaggcga cgagccgtcc gacgcaggcg
     4801 cgcctcaagg agattctcca agccgcgccg ccttcgccgc tctggtgagc ggccaaaggc
     4861 ttggcccgcc cgaggaagcg gccggccgcc ggcatggata aggaaaagca atccattgtc
     4921 gcgccggctt ttaaccagat cgaagggcta aattcgaatt tgtgtcgccg ggggcaggtt
     4981 ttacgaaccc cattaaccaa gtcgttcgca ttcccggcgc aaggtccccg cacgtccagc
     5041 gccattggcg gcgttggcga ggaggcttgc aaacaatgtc caaagctttg aagaagttca
     5101 tccgtgaaga gtccggcgcg accgccatcg aatacggcct gatcgcttcg ctgatcgccc
     5161 tcgtcattat cggcgcgatc accgtggtcg gcaccaagct gcaaacgacc ttcaatgaag
     5221 tggccagcaa tctgcactga tccaggcttt tcggggaacc tttgcgcctg acgccgggtt
     5281 gcgtatttcg gcgtcggaca tgatcccctc ctgtccgcgc cgcccctacg gccccgccga
     5341 tctctcagcg gggccgattt ttatgtcggg tctcggccgg ctggcctcgg accgacgccg
     5401 cccccggaag accggcgctc cgcccctttc gtcacggcgt cgcgccgtgt tatgggcggg
     5461 ccatgcggat gatgcgacga cgacccgccc tgccgacgcg ccgatgaccg gcgcccgccc
     5521 gcctttcccg tccccgacaa atcgttcatt cgcatgcccg acatcccgct tgtgctcgaa
     5581 gccgagacgc ccgccgacgc cgaggcggtc gagcggctga gcgcgcgcgt cttcggtccc
     5641 ggccgcttcg cccgcaccgc ctatcgcctg cgcgagacga cgccgcacga cctcaatctt
     5701 tccttcatcg cgcgggtcgg ctcgctcctg gtcggcgcca atcgcatgac gccgatcgtc
     5761 atcggcgaga cgccggcttt gctgctcggc ccgctgatcg tcgagccggt cttccgctcg
     5821 caagggatcg gcgaagcctt ggtgacgcgc tcgctcgacg cggcgcgcca ggcgggggcc
     5881 aagctcagca tcctggtcgg cgacgagccc tactattcgc gcatgggctt caaggccgcc
     5941 ccgcccggcc gcctgcaaat gcccggcccg gtcgatccgg cgcggctgct ctattgcgag
     6001 atcgcgcccg gggcttttga aggcgtgcag gggcgagtgc gcggcgcgta gatagatccc
     6061 ttctccccgc cttgcgcagg gctgtccggg ga
//
LOCUS       DATGJW010000053         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_424252, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000053 DATGJW010000000
VERSION     DATGJW010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_424252"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(573..1061)
                     /gene="rnhA"
                     /locus_tag="VKU03_01545"
     CDS             complement(573..1061)
                     /gene="rnhA"
                     /locus_tag="VKU03_01545"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HI"
                     /protein_id="HLJ69969.1"
                     /translation="MSGDGRVAIWTDGACSGNPGPGGWGAILRFGTREKELSGGEAST
                     TNNRMELMAAISALEALSRPCSVDLHTDSQYLRGGITEWLARWKRNGWRTAEKKPVKN
                     EELWRRLDEAAQRHDIHWKWVRGHAGDAMNERADALARRGMAPYLGKGRAGALGPSEA
                     SR"
     gene            complement(1058..2023)
                     /locus_tag="VKU03_01550"
     CDS             complement(1058..2023)
                     /locus_tag="VKU03_01550"
                     /EC_number="2.7.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004413 - homoserine kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine kinase"
                     /protein_id="HLJ69970.1"
                     /translation="MAVYTEVSDDALAAFLADYELGAVQSFKGIAEGVENSNFLLVCE
                     SGRYILTLYEKRVKKDDLPFFIGLMEHLAQRGVTCPLPVRSRGGEALGRLADRPAAIV
                     TFLNGLWTRRPTARHCAKVGEALATMHRAGEGFALHRPNALSIGGWPGLLAAAEARAD
                     EVAPGLAREAAQALAQLERAWPSGLPEGVIHADLFPDNVFFLGETLSGLIDFYFACND
                     AFAYDLAVCLNAWCFEPDASFNITKGMALIGEYEKVRRLEAGEVEALPILARGAALRF
                     MLTRLVDWLNVPAGAKVVPKDPLEYLKKMRFHAAIKSAREYGLAR"
     gene            complement(2252..>3159)
                     /gene="ispH"
                     /locus_tag="VKU03_01555"
     CDS             complement(2252..>3159)
                     /gene="ispH"
                     /locus_tag="VKU03_01555"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051745 - 4-hydroxy-3-methylbut-2-en-1-yl
                     diphosphate reductase activity [Evidence IEA];
                     GO_process: GO:0009240 - isopentenyl diphosphate
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0015968 - stringent response [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HLJ69971.1"
                     /translation="GVVRAIDVVERALALYGPPVYVRHEIVHNRYVVESLKKKGAIFI
                     EDLDEAPNSPAPVIFSAHGVPKSVPAEAEARGLVTIDATCPLVTKVHREAQVHFKRGR
                     KIILVGHAGHPEVIGTMGQLPPGVITLVDSVEEVEALRFDDGQDLAYITQTTLSVDDT
                     RDVVEAILKRFPAIHGPPLADICYATTNRQEAIKRAAPMVEAAIVVGSANSSNSQRLR
                     EVAERAGVEKTQLAAGAADIDWNIFGAISSLAVTAGASAPEILVEEVIAAFEERYDIE
                     VETLSTADESVFFPLPRTLRAPASA"
BASE COUNT          556 a         1031 c         1048 g          524 t
ORIGIN      
        1 ggcgttccag gagcaaagcc gaggcgccgg cgaagccgaa gctcattccg agcaggatca
       61 ggctccacaa aaaatgcggg aggcctggcc gaggcgggac ctgcgcggag agccgaacca
      121 ccacgcgcca atttagaacg attatcgtcg gcggatcatg agcttaggat aaaagtacga
      181 attatttctt cttcaaaggg tcttccccgt gtataatcct tgccgctaga aaaggtacgc
      241 cgccggagaa ccgagccgag gccaggaagc gcgagaggac ggaaaatcgc aatttccaaa
      301 ttttttgccc cctaactgct tggaatcgcc caaaatggcc aaataaaaat ttggagaaat
      361 ttgcaaaaat ttcgccggcg gcactgcggc gtcattgatc ggaacgggaa ttttgggacg
      421 cggaatttga ccgagcggcg cgtttcgcgc catccaagga ttgtccaaat ctttgccccg
      481 cgcgggattt gtccggccgt cgaccgcgcg aaggggtcgg cgcgaggcgc gaaggtacgc
      541 cgctcttgcg agcctgcgcc ctcggcgggc ccctatctcg atgcttcgct cggcccgagc
      601 gcgccggcgc ggcccttgcc gagatagggc gccatgccgc ggcgggcgag ggcgtcggcg
      661 cgctcgttca tcgcgtcgcc ggcatggccg cgcacccatt tccagtggat gtcgtggcgc
      721 tgcgccgcct cgtcgagccg gcgccacagc tcctcgttct tgaccggctt cttctccgcc
      781 gtgcgccagc cgttgcgctt ccagcgggcg agccattcgg tgattccgcc gcgcagatat
      841 tgcgaatcgg tatggagatc gaccgagcaa ggccggctca gcgcttcgag cgccgaaatc
      901 gccgccatca gctccatgcg attgttggtg gtcgacgctt cgccgccgga caattccttc
      961 tcgcgcgtcc caaagcgcaa gatcgcgccc cagccgccgg gcccgggatt gcccgagcag
     1021 gcgccgtcgg tccagatcgc gacgcgtccg tcgccgctca tcgggcgagg ccatattcgc
     1081 gggcgctttt gatcgcggcg tggaagcgca tcttcttgag atattcgagc gggtccttcg
     1141 gcacgacctt ggcgcccgcc ggcacgttga gccaatcgac cagccgggtc agcatgaagc
     1201 gcagcgcggc gccgcgcgcc aggatcggca gcgcctcgac ctcgcccgcc tcaagccgcc
     1261 tcaccttctc gtattcgccg atcagcgcca tgcccttggt gatgttgaac gaggcgtcgg
     1321 gctcgaagca ccaggcgttg aggcagacgg cgaggtcata ggcgaaggcg tcgttgcagg
     1381 cgaaatagaa atcgatcagg ccggaaagcg tctcgccgag aaagaacacg ttgtcgggaa
     1441 agaggtcggc atggatgacg ccttccggca ggccgctcgg ccatgcgcgc tcgagctggg
     1501 cgagcgcttg cgccgcctcg cgggcgaggc ccggcgcgac ttcgtcggcg cgcgcttcag
     1561 ccgccgccag cagacccggc cagccgccga tcgacaaggc gttcggccga tgaagcgcga
     1621 agccctcgcc ggcgcgatgc attgtcgcca gggcttcgcc gaccttggcg caatggcgcg
     1681 ccgtgggacg gcgcgtccac aggccgttga ggaaggtcac gatcgccgcc ggccggtcgg
     1741 ccaggcggcc aagcgcctcg ccgccgcggc tgcgcaccgg cagcggacac gtgacgccgc
     1801 gctgggcgag atgctccatc aggccgatga agaagggcag gtcgtccttc ttcacccgct
     1861 tttcgtagag cgtcaggatg tagcggccgc tttcgcagac gagcaggaaa ttcgagttct
     1921 cgacgccctc ggcgatgccc ttgaacgact gcaccgcgcc gagctcgtag tcggcgagga
     1981 acgcggcgag cgcatcgtcg gagacttcgg tatagaccgc caagatcagg ctcgatcggc
     2041 gttcgcttgg cggccgcgtg aggtacaagc ctcgcggcca accctcatcc ggcgcttcgc
     2101 gccaccttct cccgcaagcg ggagaaggga ttgacgaccc ccggcgaatt cgtcgaggtt
     2161 catgacggcc gcggcgcgcc ccctctcccg cttgcgggag agggttgggg tgagggcaag
     2221 ccgcatgctt ctatccccag cgtgatcggg cttaagcgga ggcgggggcg cgcagcgtgc
     2281 gcggcagcgg gaagaagacg ctctcgtcgg cggtcgacaa ggtttcaacc tcgatgtcgt
     2341 agcgctcctc gaaagccgcg atcacctcct cgacgagaat ctccggcgcc gaagccccgg
     2401 cggtgacggc gagggacgag atcgcgccga aaatattcca atcgatatcg gccgcgccgg
     2461 ccgcgagctg ggtcttctcg acgcccgcgc gctcggcgac ctcgcgcagc cgctgcgaat
     2521 tggacgaatt ggccgaaccg acgacgatcg ccgcctcgac catcggcgcg gcgcgcttga
     2581 tcgcctcctg gcgattggtc gtcgcatagc aaatgtcggc gagcggcggc ccgtgaatgg
     2641 cgggaaagcg cttgaggatc gcctcgacga catcgcgcgt atcgtcgacg gagagagtcg
     2701 tctgggtgat ataggcgaga tcctgcccgt cgtcgaagcg cagcgcttcg acctcctcga
     2761 ccgagtcgac gagcgtgatc acgcccggcg gcaattgccc catcgtgccg atgacttccg
     2821 gatggccggc atggccgacc aggatgatct tgcggccgcg cttgaaatgg acctgcgcct
     2881 cgcgatgtac tttcgtcacc agcgggcagg tggcgtcgat cgtgacaaga ccgcgcgcct
     2941 cggcctcggc cggcacgctt ttcggcacgc catgggccga gaagatgacc ggcgcgggag
     3001 agttcggcgc ctcgtcgaga tcctcgatga aaatggcgcc cttcttcttc agggattcga
     3061 cgacatacct gttatgcacg atttcatggc gcacatagac gggcggccca tagagcgcga
     3121 gcgcgcgctc gaccacgtcg atcgcccgca ccaccccgg
//
LOCUS       DATGJW010000054         3433 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_528694, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000054 DATGJW010000000
VERSION     DATGJW010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3433)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3433)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3433
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_528694"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..785)
                     /locus_tag="VKU03_01560"
     CDS             complement(90..785)
                     /locus_tag="VKU03_01560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ69972.1"
                     /translation="MTDLLAVDHLSAGYGEARVITGLDLRLGEGRSLAVLGRNGAGKT
                     TLINSLIGVTTRHAGRIAFAGVDVTALSPYQRARAGLGWVPQERNIFRSLSVEENLTA
                     VALPGPWTLKRVYSMFPRLKERRRNGGGQLSGGEQQMLAIGRALMTNPRLLILDEATE
                     GLAPLVRAEIWHALAALKAAQQSILVIDKNVAALTRIADRHYVLERGQIVWRGSSAEL
                     AADVELQHRYLGV"
     gene            complement(778..1533)
                     /locus_tag="VKU03_01565"
     CDS             complement(778..1533)
                     /locus_tag="VKU03_01565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ69973.1"
                     /translation="MNAPALETRALSRRFGGLTASDNVSLKLEQGARHALIGPNGAGK
                     TTLVNLLTGTLRPSSGAVLLDGADVTGLSAHARVRRGLARTFQINQLFADLTPAESLC
                     LAVGERRGHGRNGLSSAGRDRTIVTEVAALIERFGLGDVMAQKTRTLAYGKQRLLEIA
                     LALACRPRVLLLDEPAAGVPDDERADILAALAALPAEVTILLIEHDMDLVFRFAERIS
                     VLVGGRLLMEGAPAEVAADKDVRAVYLGESGHD"
     gene            complement(1530..2537)
                     /locus_tag="VKU03_01570"
     CDS             complement(1530..2537)
                     /locus_tag="VKU03_01570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ69974.1"
                     /translation="MSAIAAADAVAAHLNRGARWRPIEIVFWLAVVAAILALPSRALL
                     INEILLAGLFALSLDLVLGYAGIVSLGQAAFFGTGAYVAGIVANAGVAEPLLGLVISG
                     IAAGALGLVTAPLLLRGSDLTRLMVTLGIALLLGEVANRNSWITGGADGLSLAAAPVL
                     GLFPIGFTGQRNAAFYSLIVLFVLFILARRLMQSPFGLSVRAVKENRLRAGALGVSAA
                     SRLIAIYAIAAAYAGTAGALVAQTTGLVSLDVFDFHRSADVMLMLIIGGAGYLYGGLI
                     GAAFFIALRDGVASATPEYWEFWIGLPLVILVLIGRERIGVWIAKSAQRAAVLVGRGA
                     K"
     gene            complement(2534..3394)
                     /locus_tag="VKU03_01575"
     CDS             complement(2534..3394)
                     /locus_tag="VKU03_01575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ69975.1"
                     /translation="MLTILFDGVAYGMLLFVLACGLSVTLGLMNFVNLAHGAFAMAGG
                     YAAAVLMNRYGVPFLATLPAAFAFAAILGVALERTLYRHLYGASHLDQVLFTIGLVFM
                     AMPLANYLLGDQPQPVHVPAFLSGRFEIAGMGIGVYRLFIIAICGAIAGALQVFLIGT
                     RFGAQLRAAVDDRRVASGLGIHVDRLFAITFAIGSGLAGLGGALAADVVGGIDPSFPV
                     KFLVSFLIVVAIGGSNGIVGSLLGALLLGILDVAGKYYVPSIGAFIIYAVMVAALIIR
                     PQGLFSRSGR"
BASE COUNT          653 a         1124 c         1202 g          454 t
ORIGIN      
        1 ctcaatgacg gtgatgatct ccaccgcgcc gctcccttgg ccttaccagt gcaaaatatg
       61 cggatgccgc gcatcctcgt caatggacgc tacaccccga gatagcggtg ctgcagctcg
      121 acatcggcgg cgagctcggc cgaggagccg cgccagacga tctggcctct ttccagcacg
      181 tagtggcggt cggcgatgcg ggtgagcgcg gcgacgttct tgtcgatcac caggatcgat
      241 tgctgcgccg ccttgagcgc ggcgagcgca tgccagatct cggcgcgcac cagcggcgcc
      301 agcccttccg tcgcctcgtc gaggatgagg agccgcggat tggtcatcag cgcccgtccg
      361 atcgccagca tttgctgctc gccgcccgag agttggccgc cgccattgcg ccgccgctct
      421 ttcaggcgcg gaaacatcga atagactcgt ttaagggtcc aggggccggg cagggcgacg
      481 gcggtcaggt tttcttcgac gctcagcgag cggaagatgt tgcgctcctg cgggacccag
      541 ccgagcccgg cgcgggcgcg ctggtagggg ctgagcgccg taacgtccac gccggcgaaa
      601 gcgatccgcc cggcatggcg cgtcgtgacg ccgatcaggg aattgatcaa ggtcgtcttg
      661 ccggcgccgt tgcggccgag cacggcgagc gagcgcccct cgccgagcct cagatcgagc
      721 ccggttatga ccctggcttc gccgtagccg gcgctcaaat ggtcgaccgc gagcaggtca
      781 gtcatggccg ctctcgccga gatagacggc gcgcacgtct ttgtcggccg cgacctccgc
      841 cggcgcgccc tccatcagca ggcggccgcc gaccaggacc gaaatgcgct cggcgaagcg
      901 gaagacgagg tccatatcgt gctcgatgag caggatcgtc acctccgccg gcagcgcggc
      961 gagggcggcg agaatgtcgg cgcgctcgtc gtccggcacg cccgcggccg gctcgtcgag
     1021 cagcaggacg cgcggccggc aggcgagggc gagggcgatt tcgaggagcc gttgcttgcc
     1081 ataggcgagc gtgcgggtct tttgcgccat gacgtcgccg agaccgaagc gctcgatgag
     1141 cgccgcgact tccgtgacga tcgtccggtc gcggccggcg gaggagaggc cgtttcggcc
     1201 atggccgcgc cgctcgccga ccgcgaggca cagcgattcg gccggagtca ggtcggcgaa
     1261 gagctggttg atctggaatg tgcgcgcgag gccgcgacgt acccgcgcat gagcggaaag
     1321 gccggtcacg tccgcgccgt cgagcaggac cgcgccggag ctcggccgca acgtccccgt
     1381 cagcaaattg accaaagtcg tcttgccggc gccgttcggc ccgatcagcg catggcgcgc
     1441 gccttgttcg agcttcagcg agacattatc cgaggcggtc aggccgccga agcggcgcga
     1501 cagcgcgcgc gtctccaagg ccggcgcgtt cacttcgcgc cccgtccgac caggacggcg
     1561 gcgcgttgcg cggatttggc gatccagacg ccgatccgct cgcgtccgat cagcaccaga
     1621 atgaccagcg gcaggccgat ccagaattcc caatattcgg gcgtcgcgct ggcgacgccg
     1681 tcgcgcagcg cgatgaagaa ggcggcgccg atcagcccgc catagagata gcccgcgccg
     1741 ccaatgatca gcatcagcat cacgtcggcg gagcggtgga aatcgaacac gtccagcgac
     1801 acaagcccgg tcgtctgggc gaccagcgcg ccggcggtcc cggcgtaagc ggcggcgatc
     1861 gcatagatcg cgatcaggcg gctcgccgcg gagacgccga gcgcgccggc gcgcaggcgg
     1921 ttctccttga ccgcgcgcac ggagaggccg aagggcgatt gcatcagccg ccttgcgaga
     1981 atgaaaagta cgaagaggac gatcaagctg tagaatgcgg cgttgcgctg gccggtaaag
     2041 ccgatcggaa agaggccgag gacgggcgcg gcggcgaggc tcaaaccgtc agcgccgccg
     2101 gtgatccagg aatttcggtt ggcgacttcg ccgagcagga gcgcaatgcc gagcgtcacc
     2161 atcagccgcg tcagatcgga gccgcgcagc aagagcggcg cggtgacaag gccgagcgcg
     2221 ccggcagcga tcccgctgat gacgaggccg agcagcggct cggccacgcc ggcattggcg
     2281 acgatgcccg cgacataggc cccggtcccg aagaacgccg cttggccgag cgagacgatc
     2341 ccggcatagc cgagcaccag atcgagcgac aaggcgaaca ggccggcgag caggatttcg
     2401 ttgatcagca gcgcgcgcga cggcagcgcc agaatggcgg cgacgacggc cagccaaaag
     2461 acgatctcga tcgggcgcca gcgcgcgccg cgattaagat gggccgcgac cgcatccgcc
     2521 gcggcgatcg cgctcaccgg ccgctccgcg agaacaggcc ttgcggccgg atgatcaggg
     2581 ccgcgaccat caccgcatag atgatgaagg cgccgatcga agggacgtag tatttgccgg
     2641 cgacgtccag aatcccgagc agaagcgcgc cgagcagcga gccgacaatc ccgttggagc
     2701 cgccgatcgc cacgacgatc aggaacgaga ccaggaattt gaccggaaag ctcggatcga
     2761 tgccgccaac cacgtcggcg gcgagcgcgc cgccgagccc ggccagcccg ctgccgatcg
     2821 caaaggtgat cgcgaacagc cggtcgacat ggatgccgag gccgctggcg actcggcggt
     2881 cgtcgaccgc ggcgcgcaat tgcgcgccga atcgggtgcc gatcagaaag acttgcaggg
     2941 cgccggcaat ggcgccgcag atcgcgataa tgaacagccg atagacgcct attcccatcc
     3001 cggcgatttc aaaccggcct gaaagaaaag ccggcacatg gaccggctgg ggctgatcgc
     3061 caagcagata attggcgagc ggcatggcca tgaagacgag gccgatcgtg aataggacct
     3121 ggtcgagatg gctcgcgcca tagagatggc ggtacaaggt tcgctccagc gcgaccccga
     3181 gaatagcggc gaacgcaaag gcggcgggca aggtcgccag aaacgggacg ccgtaacgat
     3241 tcatcaggac cgcggcggcg tagccgccgg ccatggcgaa agcgccatgg gcgaggttga
     3301 cgaaattcat caggccgagc gtcaccgaca ggccgcaagc cagtacaaag agcagcatgc
     3361 cgtacgcgac gccgtcaaag aggatggtca gcacgtttca ggccccgatc ctcccccgcg
     3421 aagcggggga ggg
//
LOCUS       DATGJW010000055         2886 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_839649, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000055 DATGJW010000000
VERSION     DATGJW010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2886)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2886)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2886
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_839649"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /gene="trmFO"
                     /locus_tag="VKU03_01580"
     CDS             complement(<1..535)
                     /gene="trmFO"
                     /locus_tag="VKU03_01580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HLJ69976.1"
                     /translation="MKPVHVIGGGLAGSEAAWQIAKAGVPVVLHEMRPSRGTEAHKTG
                     GLAELVCSNSFRSDDSETNAVGIIHREMRAMGSLIMAAADAHQVPAGGALAVDRDGFS
                     AAVEAAIRARPEIAIERDEIAGLPPEDWDSVIVATGPLTSPALAAAIRTLTGEEQLAF
                     FDAIAPIVHRDSIDMDKA"
     gene            complement(706..867)
                     /locus_tag="VKU03_01585"
     CDS             complement(706..867)
                     /locus_tag="VKU03_01585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HLJ69977.1"
                     /translation="MTPFKTLAEKLNAWLRYREAVKELAQLSDRELNDIGIRRGDIEF
                     IVRQSATSA"
     gene            complement(1269..2180)
                     /locus_tag="VKU03_01590"
     CDS             complement(1269..2180)
                     /locus_tag="VKU03_01590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006479 - protein methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLJ69978.1"
                     /translation="MLEGLPPNNAAHRLRLVCDETRARAIVDLIVESFEPAEAAASAF
                     ESEAGAPDKRDWIVEAYFGFEPDRDALRNLIAAAAGEEAAGRIEFSGMAEADWVANSL
                     AGLAPVKAGRFLIHGGHDRARVRPNDIAIEIEAALAFGTGHHGTTRGCLLLFERVLRR
                     RRPARILDIGCGTGVLAIAAAKALRRPAAAGDIDPVAVATAAANARLNGAGELVRPVV
                     SRGAERRELRQGGPYDLIFANILARPLRRLAPSLAALTAPRGEAIVSGLLAADVAGVL
                     SSWRAQGFRLVQRLDLEGWASLLLCRA"
     gene            2706..>2886
                     /locus_tag="VKU03_01595"
     CDS             2706..>2886
                     /locus_tag="VKU03_01595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLJ69979.1"
                     /translation="MTAAASPPDATLRTPRPGSPLEVLRAFARLGVSSFGGPIAHLGY
                     FRQEFVIRRGWLDERA"
BASE COUNT          471 a          931 c         1014 g          470 t
ORIGIN      
        1 aggccttatc catgtcgatc gaatcgcgat ggacgatcgg cgcgatcgcg tcgaaaaaag
       61 cgagctgctc ttcgccggtc agcgtgcgga tcgccgcggc gagcgcgggc gaagtcagcg
      121 ggccggtggc gacgatgacg ctgtcccaat cttccggggg caggccggcg atttcgtcac
      181 gctcgatcgc gatttccggc cgggctctga tcgccgcctc gaccgcggcg gaaaagccgt
      241 cgcggtcgac ggcgagcgcg ccgccagccg gcacctgatg agcgtcggcc gccgccatga
      301 tcagcgagcc catcgcgcgc atctcgcggt gaatgattcc gaccgcgttg gtttcggaat
      361 cgtccgagcg gaacgaattg gagcagacga gctcggcgag gccgcccgtc ttatgcgcct
      421 cggtgccgcg cgaaggtcgc atttcgtgca ggacgaccgg gacgccggcc ttggcgattt
      481 gccaggcggc ctccgagccg gcgaggccgc cgccgatgac atggacgggc ttcatgcgga
      541 tgaattgggc ccttcggcgc aagccgtcaa tgcggccggc ggcggacagc ccggcaaata
      601 aaaagcgccc gccgggggag ggtccggcgg gcgcctgaaa cgcggacggg agaggctttg
      661 ggagggagga gggaagcctc cggccggccg cgaaactgtt ttatgttacg ccgaggtcgc
      721 gctctggcgc acgatgaatt cgatgtcgcc gcggcggatg ccgatatcgt tcagctcgcg
      781 gtcgctcagc tgggccagct ccttgacggc ttcgcgatag cgcagccagg cgttgagctt
      841 ctcagcaagg gtcttgaacg gggtcattgt catgctcctg atgtctttgt tcttgcaggt
      901 cggtcggact gtgaggcgcg gcgctcattg ctgttcgctt ggcctgacgc ggtgcaacat
      961 aggaccgcgc cgggccgatg tgtagagcga tctttggccc tcagctatga tgattatgca
     1021 tgggtaattc aatcaaattt taagtaattg cggaaatccg tctgtcttct gtgtgcgaat
     1081 atccgaaaat atgccgtgaa aatgagcgcg ctgaattctt caaggccttt taggaatgcg
     1141 aaaatttcat gacgagaata tgctgcaatg cagcatagca tcgcgccgcg aaagagaatg
     1201 tggcggcgga agcgttaggt ccgggcgagc tggcatgtca gccaaatcgc ggacgctgag
     1261 ataggcggtc aggcccggca aagcaagagg ctcgcccagc cttcgaggtc cagccgctga
     1321 accaagcgga agccctgcgc ccgccatgac gagagcacgc cggcgacatc ggcggcgagc
     1381 aggccggaga cgatcgcttc gccgcgcggc gccgtcaggg cggcgaggga gggggcgagc
     1441 cgccgcagcg gccgagcgag aatgttggcg aagatcagat cgtaaggccc cccttggcgc
     1501 agctcgcggc gctcggcgcc gcgcgagacg accgggcgca caagctctcc ggcgccgttg
     1561 agccgggcgt tggcggcggc ggtcgcgacg gcgaccgggt cgatatcgcc ggccgccgcg
     1621 gggcggcgca gggccttggc ggcggcgatc gccagcacgc ccgtgccgca gccgatatcg
     1681 aggatgcggg ccggccggcg ccggcgcagc acccgctcga ataggagcag acagccccgg
     1741 gtggtgccgt gatggccggt gccgaaggcg agggccgcct cgatctcgat ggcgatgtcg
     1801 ttcggccgga cgcgggcgcg gtcatggccg ccgtgaatga ggaagcggcc cgccttgacc
     1861 ggcgcaaggc cggccagcga attggccacc caatcggcct ccgccatgcc tgagaattcg
     1921 attctgcccg ccgcctcttc gcccgccgcg gcggcgataa ggttgcgcag cgcgtcccgg
     1981 tcgggctcga agccgaaata ggcctcgacg atccagtcgc gcttgtccgg tgcccccgcc
     2041 tcgctctcga aagcgctcgc cgccgcctcc gccggctcga agctctcgac gatcagatcg
     2101 acgatcgcgc gagcgcgggt ctcgtcgcag acgaggcgca ggcggtgggc ggcgttgttg
     2161 ggcggcaggc cttcgagcat gggcggcagg gaccgatggc gggacagctc aggtcttaag
     2221 gcgatcggcc agagctgcaa agcgggcggc ttggcggcgc taatcccctg gcgctctgta
     2281 gcctgagccg cgagcggccc tcaaggccaa gcgcgcttcg cgcggcgctc cgcgagcctt
     2341 gaccgccgct cgcggctcag gctccttggc gtccaagaac tttggtgctg aagccgtcgg
     2401 cttccgacgg tttcagcacc aaagttcttg gttgcaaaaa gccgcatcgg accgggctcg
     2461 tcatccgaag ggccgaaacg aagtgaagga gtgtccgtag cccgcggctc cgcggataaa
     2521 ggaaaggcgc tccgcctcgg gcgggctacg gatgcttgac gaggccgggc cgatgtggcg
     2581 ccgcggcgct tctcactggg gcgctacatg gtcgcgatca aggggcgagc cctcatgtca
     2641 ttcgtacaaa ctcggtacga tcggttcaat tcgccgcccg cgccctccgc gcgcctcgcc
     2701 ccgccatgac cgccgcggct tctccccccg acgcgacgct gcgtacgccg cgccccggct
     2761 cgccccttga agtcctgcgc gccttcgccc ggctcggcgt ctccagcttc ggcgggccga
     2821 tcgcccatct cggctatttc cgccaggaat tcgtcatccg ccgcggctgg ctcgacgaac
     2881 gcgcct
//
LOCUS       DATGJW010000056         5918 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_113934, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000056 DATGJW010000000
VERSION     DATGJW010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5918)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5918)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5918
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113934"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(118..906)
                     /locus_tag="VKU03_01600"
     CDS             complement(118..906)
                     /locus_tag="VKU03_01600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69980.1"
                     /translation="MANGAQTSIIGAAMAAWQDALAARQRMPTLFVTAIVAIAVLDLV
                     YFALIFLSPFAGPGLLAFVVGLIYAAAQGFLLTPLAIAVHRHLLLGEVRDNYRLDTQD
                     PRFMKFFTFLAGLAIVMFVPRLIGELFSGATGHSSFGALVSLILSIVAAVIFVRNVIL
                     FPAVAVDAPGADWRNAMADTKGHSWRVFFILLCAILPPAIVVSIVLVIFFAIPLIGWL
                     LAVALQAVFGVFVVAAAAAAASRLYVDYAQQLGRPLGLASQTAA"
     gene            complement(1054..2031)
                     /locus_tag="VKU03_01605"
     CDS             complement(1054..2031)
                     /locus_tag="VKU03_01605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLJ69981.1"
                     /translation="MSEAGNSHGRRQDDRSDHGHDHDHSGHSHAHVHVPAQFGAAFAI
                     GTALNLGFVIVEAAYGVIGHSVALLADSGHNLGDVLGLLVAWTASLLVRRAPTARYTY
                     GLRGSSILAALFNAVFLLITVGAISWEAVQRFGSPEPVAGGTVMIVAAIGILINGATA
                     WLFAAGRHGDVNIRGAFLHMAADALTSAGVVAAGLVILLAGWLWVDPLVSLAINAIIV
                     WGTWRLLRETVDMSMAAVPSRIDPARVRSFLESQPGVVAIHDLHIWPISTTEIALTCH
                     IVMPAGHPGDAFLHRIAAGLAAQFRIVHPTLQVETDPQSACALAPDEVV"
     gene            2235..3035
                     /locus_tag="VKU03_01610"
     CDS             2235..3035
                     /locus_tag="VKU03_01610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citryl-CoA lyase"
                     /protein_id="HLJ69982.1"
                     /translation="MLIGKSGEAKTAIAAAAADRVLVRGRDLAKDLIGSVSFAEFYFL
                     LATGRDPTPDQRFFLDACLVALAEHGLTPSVQAARMTLAADPAALQGAVAAGILGCGS
                     VILGTADLCARALDDIFRRVEAGVSLDAAATGWARERRAAGAPAPGYGHPLHKPLDPR
                     AERLIALAQARGVAGRAVDCATRLTGIVADIWGKKLPMNVSMAIAAVLKDVDVPEALI
                     RGLPILARTAGLIAHLAEEARTPIGFFMASRAEAAIDHSEAARGGAER"
     gene            3032..4336
                     /locus_tag="VKU03_01615"
     CDS             3032..4336
                     /locus_tag="VKU03_01615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLJ69983.1"
                     /translation="MSLVDPDIESAPWERQKEADAPLYERQIAYLFAHSRFYAEKLAA
                     AGFSDAKSAGGLDAIARLPFTEKDELRRSRSADEPIGTHCTARPEDIVRIYSTSGTTG
                     APSYIPLTAGDLDVWVRTSSRSYGASGVTRGERLVSTYNAGPFVAGAAIDAFARLGLC
                     HIPVGTGNTERLVAAIEHLKPTAATTTPSYALHLIEWTRARGIDLARSSVKRLMVAGE
                     PGGGEPAMRAKLEEGWGARVTEAMGVGDISISMWGECEEKSGMHFCGRGFIHFEVIDP
                     ETGAAKEIADGAEGELILTHLVNRSAPLLRFRTRDHVRLAVGPCACGRTSPRLRCIGR
                     TDDMLIVRGVNVFPSAIREVVNEFAPLTTGAILIRPRAPGVRQDPPLPIVVEAAAESE
                     GLAEKIRARIRDKLVVTAEVTIAPPGALPRSEYKSKLVEKAG"
     gene            4346..4930
                     /locus_tag="VKU03_01620"
     CDS             4346..4930
                     /locus_tag="VKU03_01620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329328.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ69984.1"
                     /translation="MEKIQVQGVHHITLVGADRQTSIDFWEGVLGMPFVFEQPNLDNP
                     RESHIYFDPGDGRLITVFTNEERKPDPRRTPTEPGCVHHIAFTVSRAVYMQAEKRLNE
                     RGVTPAIRDRFMFDAMYFTDPLGLLIELSCYKFEPPAGATHVDVLFEAHKLRVARGAR
                     QIEETHIADAIENLVTRDRATLSGDRAPKNPYRR"
     gene            4963..5655
                     /locus_tag="VKU03_01625"
     CDS             4963..5655
                     /locus_tag="VKU03_01625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLJ69985.1"
                     /translation="MAAIKLHILKPSVNNMAARVFVRAAKLDFTEDDAWGKTRSPDFL
                     TRNPAHLTPMMEFDHLPKGVMWESCAIMQYLCNKHHLDKFYPPAPEARAMIDSAMFYI
                     IGTLYPYIARATYPALGFPQYAGEVGASEADASAKAAAQKAASAAVEEPLGVFHKFYM
                     DGKPFIGGAEPSIADIRLAASLEFLAVIDYPLPAWAKTYMGAIEKALGAAYSEPAADV
                     RGYIAHVKGQKK"
     gene            complement(5826..5915)
                     /locus_tag="VKU03_01630"
     CDS             complement(5826..5915)
                     /locus_tag="VKU03_01630"
                     /EC_number="3.5.2.17"
                     /inference="COORDINATES: protein motif:HMM:NF012785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyisourate hydrolase"
                     /protein_id="HLJ69986.1"
                     /translation="MRFAVADPKAHYHVPLLASPWSYSTYRGS"
BASE COUNT         1037 a         1963 c         2012 g          906 t
ORIGIN      
        1 aaggccggtg gagggggccg ccggcggttg ggctttatcc tgatagatcg gagccccctc
       61 caccatgctt cgcatggtcc ccctcccccg ctttgcgggt gaggaaagcg cacgtaccta
      121 cgcggcggtt tggctggcga ggccgagcgg gcggccgagc tgctgggcgt agtcgacata
      181 gagccgcgag gcggccgcgg ccgccgcggc cacgacgaag acgccgaaaa cagcctgaag
      241 ggcgacggcg agaagccatc cgatcagcgg gatcgcgaag aagatcacca acacgatcga
      301 gaccacgatc gccggcggca ggatcgcgca gagcaggatg aagaagacgc gccaggaatg
      361 gcctttcgtg tcggccatgg cattgcgcca atcggcgccg ggcgcgtcga ccgcgaccgc
      421 gggaaagaga atgacgttgc gcacgaagat caccgccgcg acgatcgaca ggatgaggct
      481 gaccagtgcg ccgaagctcg aatggcccgt ggcgccgctg aagagctcgc cgatcagccg
      541 cggaacgaac atgacgattg cgagcccggc caggaaggtg aagaacttca tgaagcgcgg
      601 gtcttgcgtg tcgagccggt aattgtccct gacttcgccg agcagaagat ggcgatggac
      661 ggcgatggcg agcggcgtca ggaggaagcc ttgcgccgcc gcgtagatca ggccgacgac
      721 aaaggccaag aggcccggcc cggcgaatgg gctgaggaaa atgagggcga aatagacaag
      781 atcgagaacg gcgatcgcaa cgatcgccgt cacgaaaagc gtcggcatgc gctgacgcgc
      841 cgcgagcgcg tcctgccaag cggccatcgc cgcgccgatg atggaagttt gtgcgccatt
      901 cgccatgacc gcccctccaa cgctcatgag cgcgccccgc ctgattgacg aggcgcgaac
      961 ggaaggggat cgcgacaatc tgccgctgtc aaggcgcttg cgccccgcgg gctcgggact
     1021 cggcggaacg gtgtggatta ctggggcttc ggctcaaacc acctcgtcgg gggccagcgc
     1081 gcaggccgat tgcggatcgg tttcgacctg cagggtcggg tggacgatcc ggaactgcgc
     1141 cgcgaggccg gcggcgattc ggtgaagaaa ggcgtcgccc gggtggccgg ccggcatgac
     1201 gatgtggcag gtcagcgcga tctcggtcgt gctgatcggc cagatatgca ggtcgtgaat
     1261 cgcgacgacg cccggctggc tttcgaggaa cgagcgcact ctggccggat cgatgcggct
     1321 cggcaccgcc gccatcgaca tgtcgaccgt ctcacgcagc aggcgccagg tcccccagac
     1381 gatgatagcg ttgatcgcca ggctgaccag gggatcgacc cagagccagc cggcgagcag
     1441 aatgacgagg ccggccgcga ccacgccggc cgaggtcagc gcgtcggcgg ccatatggag
     1501 gaaggcgccg cggatattga cgtcgccatg gcgtcccgcg gcgaagagcc aggcggtcgc
     1561 gccgttgatc aggatgccga tcgccgcgac gatcatgacg gttccgcccg cgaccggctc
     1621 gggcgagccg aaccgttgca cggcctccca cgagatcgcc ccgaccgtga tcagaagaaa
     1681 gaccgcattg aacaaggcgg cgagaatcga ggagccgcgc aggccataag tatagcgcgc
     1741 ggtcggcgcg cgccggacga gcaggctcgc ggtccaggcg acgaggaggc ccagcacgtc
     1801 gccgagattg tggccggaat cggcgagaag cgcgaccgaa tggccgatca cgccgtaagc
     1861 cgcctcaacg atcacgaagc cgagattgag cgcggtcccg atcgcgaacg cggcgccgaa
     1921 ctgcgccggg acatggacat gagcgtggct atggccggaa tgatcatggt cgtgaccatg
     1981 atcggaccga tcgtcttggc ggcggccatg cgaatttccg gcttcgctca ttctcgatcc
     2041 atcctgcagc gacgcgcggc tagattagcg caattttcca tcgcattctc gccggcgagg
     2101 caatcgcgcg gcgacggcaa ggccgcaagc tcaagcttgc cggcgcgctc taggcgcata
     2161 tcgtcgactg tcctactctc ccgggaaatg caggaaggct ctatcgtcat cgcagggagg
     2221 aaggcttaaa agccatgctg atcggcaaaa gcggcgaggc gaaaaccgcg atcgcggcgg
     2281 ccgcggccga ccgcgtcctc gtacgcggcc gcgacctcgc caaggatttg atcggctcgg
     2341 tcagcttcgc cgaattctac ttcctcctgg cgaccggccg cgatccgacg ccggaccagc
     2401 gtttcttcct cgacgcctgc ctcgtcgctc tggccgagca tggcctgacc ccttcggtcc
     2461 aggcggcccg gatgacgctc gccgccgatc cggccgcctt gcagggcgcg gtcgcggcgg
     2521 ggattctcgg ctgcggctct gtcattctcg gaacggccga tctttgcgct cgcgcgctcg
     2581 acgacatttt tcggcgcgtc gaggccggcg tttccctgga cgccgccgca accggatggg
     2641 cgcgcgagcg ccgcgccgcc ggcgcgccgg cgccgggcta tgggcatccc ttgcataagc
     2701 cgctcgatcc gcgcgccgag cggctgatcg cgctcgccca ggcgcgcggc gtcgccggcc
     2761 gggcggtcga ttgcgcgacg cgcttgaccg gcattgtcgc cgacatctgg ggcaagaaac
     2821 tgccgatgaa cgtctcgatg gcgatcgccg ccgttctcaa ggacgtggac gttcccgaag
     2881 cgctcatccg cggcttgccg atcctggcgc gcaccgccgg tctgatcgcg catcttgccg
     2941 aagaggcgcg gacgccgatc ggcttcttca tggcttcgcg agccgaggcc gcgatcgacc
     3001 attccgaggc ggcgcgcggg ggggcggagc ggtgagcctg gtcgatcccg acatcgaaag
     3061 cgcgccctgg gagcgccaga aggaggccga cgcgccgctt tacgagcggc agatcgccta
     3121 tcttttcgcc cattcgcgct tctacgccga aaagctcgcg gcggccggct tttccgatgc
     3181 gaaatcggcc ggcgggctcg atgcgatcgc gcggctgccc ttcaccgaga aggacgagtt
     3241 gcgtcgctcg cgttccgccg acgagccgat cgggacgcat tgcacggcgc ggccggaaga
     3301 catcgtgcgc atctattcga ccagcggcac caccggcgcg ccgagctata tcccgctcac
     3361 cgccggcgat ctcgacgtct gggtgcgcac gtcctcgcgc agctacggcg cgtcgggcgt
     3421 cacgcgcggt gagcgcttgg tgtcgaccta caatgccggc cccttcgtcg ccggcgcggc
     3481 gatcgacgcc ttcgcccgcc tcggcctctg tcacattccg gtcggcaccg gaaacaccga
     3541 gcggctggtc gcggcgatcg agcatttgaa gccgaccgcc gcgacgacga cgccgtccta
     3601 tgcgctccat ctcatcgagt ggacgcgggc gcgcgggatc gacctcgccc gatccagcgt
     3661 caagcggctg atggtcgccg gtgagccggg cggcggcgag ccggccatgc gcgccaagct
     3721 cgaggaaggg tggggcgcgc gcgtcaccga agcgatgggc gtcggcgaca tttcgatctc
     3781 gatgtggggc gagtgcgaag aaaaaagcgg catgcatttc tgcggccgcg gctttatcca
     3841 tttcgaagtg atcgatcccg agaccggcgc ggcgaaggag atcgccgacg gcgcggaagg
     3901 cgagctgatt ctgacccatc tcgtcaaccg ctccgcgccg ctgctgcgct tccggacgcg
     3961 cgatcatgtc aggctggcgg tcggcccttg cgcctgcggc cgcacgtcgc cgcgcctgcg
     4021 ctgcatcggc cggaccgacg acatgctgat cgtgcgcggc gtcaatgtct ttccctccgc
     4081 catccgcgaa gtcgtcaacg aattcgcgcc gctgaccacc ggcgcgatcc tcattcgccc
     4141 gcgcgcgccc ggcgtgcgcc aggacccgcc gctgccgatc gtggtcgagg cggcggcgga
     4201 gagcgagggc ctggcggaaa aaatccgcgc gcgcatccga gacaagctcg tcgtgaccgc
     4261 cgaggtgacg atcgccccgc cgggcgcgct gccgcgcagc gagtacaagt ccaagctcgt
     4321 cgagaaagcc ggataggagg cggcgatgga gaaaatccag gtccagggcg tccaccacat
     4381 cacgctcgtc ggcgccgacc gccagacgtc gatcgacttc tgggagggcg tgctcggcat
     4441 gcctttcgtc ttcgagcagc ccaatctcga caatccgcgc gagagccaca tctatttcga
     4501 tcccggcgac gggcgactga tcaccgtctt caccaatgaa gagcgcaagc ccgatccgcg
     4561 ccgcacgccg accgagccgg gctgcgtcca ccacatcgcc ttcactgtgt cgcgcgccgt
     4621 ttatatgcag gcggagaagc ggctgaacga gcgcggcgtg acgccggcga tccgcgaccg
     4681 cttcatgttc gatgcgatgt atttcaccga tccgctcggc ctgctgatcg agctgtcctg
     4741 ctacaagttc gagccgcccg cgggcgcgac gcatgtcgat gttcttttcg aagcccataa
     4801 gctgcgcgtc gcgcgcggcg cgcggcagat cgaggagacg catatcgccg acgcgatcga
     4861 aaatctggtc acgcgcgatc gcgcgacgct gtccggcgat cgcgcgccga agaacccgta
     4921 ccggcgctaa aggcgccggt tcatcttgca agggaggaag cgatggctgc gatcaagctg
     4981 catattttga agccgagcgt gaacaatatg gcggcgcgcg tcttcgtccg cgccgccaag
     5041 ctcgatttca ccgaggacga cgcctggggc aagacccgct cgcccgattt cctgacgcgc
     5101 aatccggctc atctgacgcc gatgatggag ttcgaccatc tgccgaaagg ggtgatgtgg
     5161 gagagctgcg cgatcatgca gtatctctgc aacaagcatc atctcgacaa gttctatccc
     5221 cccgcgccgg aagcgcgcgc gatgatcgac agcgcgatgt tctacattat cggcacgctc
     5281 tacccgtaca tcgcccgtgc gacctatccg gcgctcggct tcccgcaata tgccggcgag
     5341 gtcggggcga gcgaggccga cgcttccgcc aaggcggccg cgcagaaggc ggcgagcgct
     5401 gcggtggagg agccgctcgg cgtgttccat aagttctata tggacggcaa gccgttcatc
     5461 ggcggcgccg agccgtcgat cgccgacatc cggctcgccg cctcgctcga attcctcgct
     5521 gttatcgact acccgctgcc ggcctgggcc aagacctata tgggggcgat cgagaaggcg
     5581 ctgggcgccg cctattccga accggccgcc gacgtgcgcg gctacatcgc ccatgtgaag
     5641 ggacagaaga agtaaacgct taccctctcg ccacgaacgc tgcggcttgc cctcacccca
     5701 gccctctccc gcaagcggga gagggggcgc gccgcgcgct ttatcgatgc tgcggatata
     5761 gggcggcggc gtacccgccg cgctgtgccg cggtcgccga ccgcggctac aatcctcgcc
     5821 tggctttagc tgccgcgata ggtcgaatag ctccacgggc tcgccagaag cggcacgtgg
     5881 taatgcgcct tgggatcggc gacggcgaag cggatcgg
//
LOCUS       DATGJW010000057         9732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_943776, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000057 DATGJW010000000
VERSION     DATGJW010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9732
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943776"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1124
                     /locus_tag="VKU03_01635"
     CDS             <1..1124
                     /locus_tag="VKU03_01635"
                     /inference="COORDINATES: protein motif:HMM:NF006775.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016813 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear
                     amidines [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="allantoate amidohydrolase"
                     /protein_id="HLJ69987.1"
                     /translation="VAWCEEAGLRAKIDASGNVWARYEGKTAGAPALMLGSHIDTVRN
                     AGRYDGNLGALAALAVVEELAAKDERLDHAIEIVAFGDEEGVRFPTTLTGSRALAGLF
                     ARSALDQQDADGVAMRDALRAFGCDPEAVADLTARHVAAFVELHIEQGPVLESENIAV
                     GVVTAINGQTRFQAIVRGEAGHAGAVPMGLRRDALAAAAEMMLSIEQRARSEGDLVAT
                     VGRLDVAPGAVNVIPAEARFTIDIRAPGDEQRRRAAEDVSKRLAAIAAARRVELLLTQ
                     THEAGAYTCHPAIVAALKESVERTSGRPARLLPSGAGHDTMIMGQLCPAGMLFVRCKG
                     GISHNPAESITTEDAEMGLAALIDFTRRFRAAAPAHATP"
     gene            1322..1582
                     /locus_tag="VKU03_01640"
     CDS             1322..1582
                     /locus_tag="VKU03_01640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69988.1"
                     /translation="MHTHHHRANAPNHRANAPNVGALDAASLEENNEIRRRFGATPIY
                     VLRQSYGEGFAPGTPGDLTLAQAIDHIDAASLHLLARRLNRM"
     gene            1842..3149
                     /gene="xylA"
                     /locus_tag="VKU03_01645"
     CDS             1842..3149
                     /gene="xylA"
                     /locus_tag="VKU03_01645"
                     /EC_number="5.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0009045 - xylose isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xylose isomerase"
                     /protein_id="HLJ69989.1"
                     /translation="MTDFFGKIAPIRYEGPQSDNPLAFRHYDAGRKVFGKTLAEQLRP
                     AVCYWHNFAWTGVDMFGAPTFERPWFGETMQAAKLKADVAFEMFSLLGAPFFTFHDRD
                     VVPEGESLAEFGRNLAEITEYFAAKMTASGVKLLWGTANLFSHARYMAGAATNPDPDV
                     FAYAAAQVKNVLDATHRLDGANYVLWGGREGYETLLNTDLRREQEQLGRFLSLVVDYK
                     HKIGFKGVILVEPKPQEPTKHQYDYDVATVFGFLQRFGLEKEVKVNIEVGHAVLAGHS
                     FEHEVAMAVALDIFGSLDLNRNDYQSGWDTDQFPNNAAELIPAFYHILKGGGVAPGGL
                     NFDAKLRRQSIDPEDLLIAHIGGLDICAQALIAAAKLIEDGKYDSALAQRYAGWSEPE
                     ARAMLDGQRSLEDIAKRVAEKKISPKPRSGKQEFFENLINRYV"
     gene            3384..4166
                     /locus_tag="VKU03_01650"
     CDS             3384..4166
                     /locus_tag="VKU03_01650"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ69990.1"
                     /translation="MSNAIEDAPIATYPSLKGKVVLITGGGGGIGAAITRRFAEQGAK
                     TAFIDIAEAPSKALAETLSGAGREVHFEKADLKNIEALRGAVAAIRKRFGPIDILINN
                     AAHDERHAMETVTPEYFDDRIAVNLRHQFFMAQAVLPDMVEKKKGVIVNFGSTSWMLG
                     RGGMPVYTASKSAVLGLTRSIARDYGPHNVRCLAIAPGWIMTERQMTLWLTPEGEAEL
                     MNGQCVKRKLVPDDMARVVLFFASDEAAAMTNQQYVADGGWL"
     gene            4182..5078
                     /locus_tag="VKU03_01655"
     CDS             4182..5078
                     /locus_tag="VKU03_01655"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HLJ69991.1"
                     /translation="MIVVFGSINLDLITRVERIPGPGETTLGGDYATSPGGKGANQAL
                     AARRAGAQVAMVGAVGKDAFATEALALLTADSVDLSATEAVAAPTGAAFIAVDANGEN
                     AIVVASGANRLAKAAQLEKLAIGAGDILVLQRETPPAEVEAAALHAKSRGARVILNLA
                     PAGPPGDKLLRALDALIVNEHEALTLGEALKLATREPEEVAAALDRRLGLAAIVTLGP
                     AGAVGYAKGARFAAPAPPVKVVDTTGAGDTFVGAFAAALERGLSFEAAMKRGLAAGSL
                     ACTKPGAQPSMPYAAEIEALAK"
     gene            5154..5801
                     /locus_tag="VKU03_01660"
     CDS             5154..5801
                     /locus_tag="VKU03_01660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLJ69992.1"
                     /translation="MKTALCYGDSNTWGCVPMASLDDDARHEESRRWPKIMGKALGAD
                     WRAIAEGLPGRTTVHDDPVEGGHLSGLAYLRPCLESHRPLDVVVLMLGTNDFKRRFCL
                     EAEEIAQGIERLMVEISHLDVFGGKEERIFVICPPPIAVVGPLATMFQDADRKSRQLA
                     PLLRDIARRRGAGFLDAGEIVRSSPIDGIHLDADAQVALGRAVAAALPAFIAKAA"
     gene            5912..6454
                     /locus_tag="VKU03_01665"
     CDS             5912..6454
                     /locus_tag="VKU03_01665"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HLJ69993.1"
                     /translation="MLRSTLLVAILACVLAAYAPTLFPALLQAMSEKTSAPQPLPVVA
                     PAPPPAAESGDNDERRVEIDADAGGQYSTEVAVNGVVVHMLVDTGATTVALSADTAQR
                     LGLPLSDASYTALVRTANGVARAAPVILSAISIGDIYVPAVQGVVFERKAGHVDLLGM
                     SFLKRLSSVEQKSGRLILRQ"
     gene            6722..7120
                     /locus_tag="VKU03_01670"
     CDS             6722..7120
                     /locus_tag="VKU03_01670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69994.1"
                     /translation="MKKMSLTLGGATLLALAGFAVLPARAADKVLMGHTVACDSAQEV
                     KSFVGGKSDEQAKTALNRVNRRYGKDACSVETLVFAPEKMAGDVITPAGLVEIMKVEV
                     LGVVDGSHLKEFMQPITQYVPAIDQGGLEV"
     gene            7254..8000
                     /locus_tag="VKU03_01675"
     CDS             7254..8000
                     /locus_tag="VKU03_01675"
                     /inference="COORDINATES: protein motif:HMM:NF024430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3891 family protein"
                     /protein_id="HLJ69995.1"
                     /translation="MLFRDDGSEVLAVSQLSHAWIAGQILRAWRENLGERLLFATELH
                     DIGWLDWEAAPTFNPQTGRPHLFREVAPAVHAPMWTQGVERALAGWGAHVALLVSRHG
                     GLIYRRYARRGDGADAAAVDNYLAAQAAFEGIWAGKLGLSDEALNKETALLAFADALS
                     LALCGDLKAPLDLEAPGHDGETVRFRLEQLGRPFEFTLSPWPFREAVLIVEGEGRPLP
                     TGGRFPSEAAMREWLEAPRRASFQSILRRA"
     gene            complement(8134..8463)
                     /locus_tag="VKU03_01680"
     CDS             complement(8134..8463)
                     /locus_tag="VKU03_01680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69996.1"
                     /translation="MAVCVQPGTELAFDNEVLTSYDFSFLRRFWRRKSRGKVAQFRQV
                     NMHRPNVHHDALEFPDGEIVMLTSLRPGQHARVLQLPAKPSDSTGAPAEEQGVFELHR
                     RLQPDRV"
     gene            8851..9579
                     /locus_tag="VKU03_01685"
     CDS             8851..9579
                     /locus_tag="VKU03_01685"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ69997.1"
                     /translation="MSEPEPSSPAYDVSEAALKELYRDLDADRADVMKLIDEAEALIE
                     SNLERKPGSNKREARAAADIASKPAADSLPKPTDDVGHDALVRPRRRRRAGAGLALGL
                     VLLLVGFVAYAPFRSRLTSDVPRRPPSITGFLRVADLAAGHTIKPGDVYAVDIPSDAV
                     RIKVLSGRVALVTKNGASLRACSDQPFVIEIKHGAPSAANPLLSGCGGAPSRIQAVIP
                     QPPLPSPVPAPAISPALLKRQDRD"
BASE COUNT         1669 a         3170 c         3225 g         1668 t
ORIGIN      
        1 tcgtcgcctg gtgcgaagag gcgggattgc gagccaagat cgacgcgtcg ggcaatgtct
       61 gggcgcgcta tgaaggcaag acggccggcg cgccggcgct gatgctcggt tctcatatcg
      121 acaccgtgcg caatgccggc cgctatgacg gcaatctcgg cgcgctggcg gccttggccg
      181 tggtcgagga gttggcggcg aaggacgagc ggctcgacca cgcaatcgag atcgtcgcgt
      241 ttggcgacga ggagggcgtg cgctttccga ccacgctgac cggctcgcgc gctctcgccg
      301 gcttgttcgc ccgttccgcg ctggaccagc aagacgccga cggcgtcgcg atgcgcgatg
      361 ctctccgggc tttcggttgc gatcccgagg cggtggcgga tttaacggcg cgccacgtcg
      421 ccgccttcgt cgaattgcat atcgagcaag gaccggtcct cgaaagcgaa aatatcgcgg
      481 tcggcgtcgt gacggcgatc aacggccaga cgcgcttcca ggcgattgtt cgaggcgagg
      541 ccggccatgc cggcgccgtg ccgatggggc tgcgccgcga cgcgctcgcg gcggcggccg
      601 agatgatgct gtcgatcgag cagcgcgcgc gcagcgaggg cgacctggtc gccaccgtcg
      661 ggcggctcga cgtcgccccc ggcgcggtca atgtcattcc tgccgaagcg cgcttcacca
      721 tcgacatccg cgcgccaggc gacgagcagc gccggcgcgc cgccgaggat gtctccaaac
      781 ggctcgcggc gatcgccgcg gcgcgacggg tcgagctctt attgacgcag acgcatgaag
      841 cgggcgccta tacatgtcac ccggcgatcg tcgccgcgct caaggaaagc gtcgagcgta
      901 catccggacg gccggcgcgg ttgcttccgt ccggcgctgg gcatgacacg atgatcatgg
      961 gccagctgtg cccggccgga atgctgttcg tgcgctgcaa gggcgggatc agtcacaacc
     1021 cggcagagtc gatcacaacc gaagacgcgg agatgggact ggcggctttg atcgacttca
     1081 cgcgacgctt ccgtgcagcc gctccggccc atgcgacccc ttaaaatagc cgcattggcg
     1141 ccatctcgtc gcaggagcgg cgaaagcgcg agcgggtggg cgtcacgcaa cggcgcgccg
     1201 gaacaagcgg cgcatcaaga gcgttgttac ctctaggaaa ttcgctttcg gctggagcaa
     1261 agcctccaac gcctggcgca gcgtagcgcg gcgggcgtcg ccatgaagcg ggaggatcac
     1321 catgcatacg catcaccatc gcgccaatgc gcccaaccat cgcgccaatg cgcccaatgt
     1381 cggcgctttg gacgcggcca gtctggaaga gaacaacgag attcgcagaa ggttcggcgc
     1441 aacgccgatc tacgtcctgc gccaatccta tggcgaaggc tttgcgccgg gcacgccggg
     1501 cgatcttacg cttgcccaag ccattgacca cattgacgcc gcgtcgctgc acttgttggc
     1561 gcggcgattg aaccggatgt agaccggccg cctcgtcgcc tggaccgtcg ccccgcggcc
     1621 gcgccgcggg gcttttcatt ggcgccgcgc cttgacaggc ttgcctgcgg cgaaacaaaa
     1681 tacggaatcc ggaataaatg actcggggcg ccaaaggcgg caggccggaa cgtcattggg
     1741 ccaagtgcga agcgcgcgga gctccggctt ttcgcgaaaa tatctgttga taagttgagc
     1801 cgcgaaaggc gcgcgctcgc agcggaagag tgggggaaac gatgacggat ttcttcggca
     1861 aaattgcgcc gatccgctac gaggggccgc aaagcgacaa tccgctcgcc tttcgccatt
     1921 acgacgccgg gcggaaagtt ttcggcaaga cgctcgccga gcaattgcgg ccggcggtct
     1981 gctattggca caatttcgcc tggaccggcg tcgacatgtt cggcgcgccg accttcgagc
     2041 ggccgtggtt cggcgagacc atgcaggcgg cgaagctcaa agccgacgtc gccttcgaga
     2101 tgttctcgct gctcggcgcg cctttcttca ccttccacga tcgcgacgtg gtcccggaag
     2161 gcgagagcct cgccgaattc ggccgcaacc tcgcggagat caccgagtat ttcgcggcca
     2221 agatgaccgc ctcgggcgtg aagctgctct ggggcaccgc caatcttttc agccacgccc
     2281 gctacatggc cggcgcggcg accaaccccg atcccgacgt cttcgcttat gcggcggcgc
     2341 aggtgaagaa cgtgctcgac gccacgcatc gtctcgacgg cgccaattac gtactctggg
     2401 gcggccgcga gggctacgag acgctgctca acaccgatct tcggcgcgag caggagcagc
     2461 tcgggcggtt tctgtccctg gtcgtcgact acaagcacaa gatcggcttc aagggcgtga
     2521 tcctggtcga gcccaagccg caagagccga ccaagcacca atacgattac gacgttgcga
     2581 ccgtcttcgg cttcttgcag agattcgggc tcgagaaaga ggtcaaggtc aatatcgagg
     2641 tcggccatgc ggttctcgcc ggccattcct tcgagcatga agtggcgatg gcggtcgcgc
     2701 tcgatatttt cggctcgctg gacctcaacc gcaacgatta tcagtccggc tgggacaccg
     2761 atcagttccc gaacaacgcg gccgagctga ttccggcttt ctatcacatc ctgaaaggcg
     2821 gcggcgtcgc gccgggcggg ctcaatttcg acgccaagct gcgccgccaa tcgatcgacc
     2881 ccgaggatct tctcatcgcc catatcggcg gtttggatat ttgcgcacag gcgctgatcg
     2941 cggcggcgaa gctgatcgag gacggcaaat acgattccgc gctcgcccag cgctatgcgg
     3001 gctggagcga gccggaggcc agggccatgc ttgacggcca gcgctcgctc gaagacatcg
     3061 ccaagcgcgt cgccgagaag aagatttcgc ccaagccgcg gtcgggcaag caggaattct
     3121 tcgagaatct gatcaatcgg tacgtttgat ccagccctcg ccgactgatg gcgtaaaaca
     3181 ccgctgtcag gaacccctct ccccgtgaaa cggggagagg tacggtgagg ggcaggtcgg
     3241 ctaggctcga cctcggcgtt ttacgcccca agcccctctc cgtacctctc cccgcgaagc
     3301 ggggagaggg ggccctcggc gccgcgttct taatacatgc ggcggtgaat ccgccgtcaa
     3361 actcgtcagg agtcctcttc aaaatgtcga acgccatcga agacgcgccg atcgcgacct
     3421 atccgagcct caaaggaaag gtcgtcctga tcaccggcgg cggcggcggc atcggcgcgg
     3481 cgatcacgcg ccgcttcgcc gagcagggcg ccaaaaccgc tttcatcgac atcgcggagg
     3541 cgccgtccaa ggctttggcc gagacattga gcggcgccgg ccgcgaagtc catttcgaga
     3601 aagcggacct gaagaacatc gaggcgctgc gcggcgcggt cgcggcgatc cgcaagcgct
     3661 tcggcccgat cgacattctc atcaacaacg ccgcccatga cgagcgccac gccatggaga
     3721 cggtgacgcc cgaatatttc gacgaccgca tcgcggtcaa tctcaggcat cagttcttca
     3781 tggcgcaggc cgtgctgccg gacatggtcg agaagaagaa aggggtgatc gtcaatttcg
     3841 gctcgacctc ctggatgctc ggccgcggcg gcatgccggt ctatacggcg tccaaatcgg
     3901 cggtccttgg cttgacgcgc tcgatcgccc gcgactatgg cccgcacaac gtaagatgct
     3961 tggccatcgc gccgggctgg atcatgaccg agcgccagat gacgctgtgg ctgacgccgg
     4021 aaggcgaggc cgaactgatg aacggccaat gcgtcaagcg caagctggtt cccgacgaca
     4081 tggcccgcgt cgttttgttc ttcgcctccg acgaagccgc ggctatgacc aatcagcaat
     4141 atgtcgccga cggcggctgg ctgtgaccgg aggctgaaag catgatcgtc gtcttcggct
     4201 cgatcaatct cgacctgatc acgcgcgtcg agcgcattcc ggggccgggc gagacgacgc
     4261 tgggcggcga ttatgcgacg agccccggcg gcaagggcgc caatcaggcc ctggcggcgc
     4321 gtcgcgccgg cgcgcaagtc gcgatggtcg gcgcggtcgg caaggacgct tttgcgacgg
     4381 aggcgttggc gcttttgacg gctgacagcg tcgatctttc ggcgaccgag gccgtcgctg
     4441 cgccgaccgg cgcagccttc atcgccgtcg acgctaatgg cgagaacgcg atcgtcgtcg
     4501 cctccggcgc caatcggctc gcaaaggcgg cgcaactcga gaagcttgcg atcggcgcgg
     4561 gcgacattct cgttttgcag cgcgagacgc cgccggcgga agtggaggcc gccgcgctcc
     4621 acgccaagag ccgcggggcg cgggtgatcc tgaatctcgc accggccggc ccgcccggcg
     4681 acaagctgtt gcgggcgctc gacgcgctga ttgtcaacga gcacgaggcg ctgacgctcg
     4741 gtgaagcgtt gaagcttgcg acgcgcgagc cggaagaggt cgccgccgct ctcgaccgga
     4801 ggctcggcct tgccgcgatc gtgacgctcg gtccggccgg cgccgtcggc tatgcgaagg
     4861 gcgcgcgctt cgccgccccg gcgccgccag tgaaagtcgt cgatacgacc ggcgcgggcg
     4921 atacctttgt cggcgccttc gccgctgcgc tcgaacgcgg cctatcgttt gaagccgcga
     4981 tgaagcgcgg cctcgccgcc gggagcctcg cttgcacgaa gcccggcgcg cagccgagca
     5041 tgccctacgc ggcggagatc gaggcgctgg cgaaatagag cggaggggcg agggagcgtg
     5101 gtcccgggcg ctgctgcgct cagccgagtc ctgccgaacc gagagggaag gggatgaaaa
     5161 cggctttgtg ctacggcgat tcgaacacct ggggctgtgt cccgatggct tcgttggacg
     5221 acgatgcgcg gcacgaagaa tcgcggcgct ggccgaagat catggggaag gcgctcggcg
     5281 cggattggcg ggcgatcgcc gaaggcctgc cgggacgcac caccgtgcat gacgatccgg
     5341 tcgagggcgg ccacttgtcg ggcctcgcat atctcaggcc ttgtctcgag agccatcggc
     5401 cgctcgatgt cgttgttctc atgctcggga cgaacgactt caaacggcgc ttctgcctgg
     5461 aagccgagga gatcgcgcaa gggatcgagc ggctgatggt tgaaatttca catctcgatg
     5521 tctttggcgg caaggaggag cggattttcg tcatttgccc gccgccgatc gcggtcgtcg
     5581 gcccgctcgc gacaatgttt caagacgccg atcggaagtc gcgccaactc gcgccgctgc
     5641 tgcgggacat tgcgcggcgg cgcggagcgg gattcctcga cgccggcgag atcgtccgtt
     5701 cgagcccgat cgatgggatt catctcgatg cggacgcgca ggtcgcgctc gggcgggccg
     5761 tcgcggcggc gcttcccgcc ttcatcgcca aggcggcttg acgccttttc cgacgcgtcc
     5821 ctgaaattcg ccggcttccg ttaacgcctt attcgcgggc gccgcggtaa tgtcgcgatc
     5881 tccgtccccg cacggggcct gcgcgtccgc catgttgcgt tcaacccttc ttgtcgccat
     5941 cctggcctgt gtgcttgccg cctacgcgcc gacgctcttc ccggcgctct tgcaggcgat
     6001 gagcgaaaag acgagcgcgc cgcagccgct cccggtcgtc gcgccagcgc cgcctccggc
     6061 ggccgaaagc ggcgacaatg acgaacgcag ggtcgaaatc gacgccgatg ccggcggcca
     6121 atattcgacc gaggtcgccg tcaacggcgt cgtcgtccat atgctggtcg ataccggcgc
     6181 gaccacggtg gcgctttccg ccgacacggc gcagcggctc ggcctgccgc tgagcgacgc
     6241 cagctacacg gccctggtgc gcaccgccaa tggcgtggcg cgcgccgcgc cggtcatcct
     6301 ctccgcgatc agcatcggcg acatttacgt ccctgccgtg cagggcgtgg tgtttgagcg
     6361 caaggcgggt catgtcgatc tgctcggcat gagcttcttg aaacggctct cgtccgtcga
     6421 gcagaaatcc ggacggctga ttttgcggca ataaagcgaa aagaaggtcg tcggcgtccc
     6481 cggctctgta atcgtacgca cgggccgacc tgccttggct ttccgccaag tgccgacagt
     6541 gccgggactc gggtcaaggg cggccgccgc ccgcgctgac gctcatcgag agcggcgctc
     6601 cagccgatcc cgtcgcgccg agggcgctcc cgcgctctcg cccttgtgat ggcgcgccgg
     6661 aaccggctcg aatcccggcc gtttttatca aaacagcctc gttttcaaag gatttgcggc
     6721 tatgaaaaag atgtcgctca ccctcggcgg cgcgacgctt ctcgccctcg ccggattcgc
     6781 cgtccttccg gcgcgagcgg cggacaaggt tctgatggga cataccgtcg cctgcgattc
     6841 ggcgcaggag gtcaagtcct tcgtcggcgg caaatcggac gagcaggcga agacggccct
     6901 caatcgcgtg aaccgccgct acggcaagga cgcttgcagc gtcgagacgc ttgtctttgc
     6961 gccggagaaa atggctggcg acgtgatcac gccggccggc ttggtcgaaa tcatgaaggt
     7021 cgaagtgttg ggcgtcgtcg acggctcgca cctgaaagag ttcatgcagc cgatcaccca
     7081 atacgtgccg gcgatcgatc agggcggcct ggaagtttga ccccgtgaca tgtgaccccg
     7141 tgacatgggg cgtgcgcttc ggcaaagcgg tatagttcgc cggcgcccgg gcaggtcacg
     7201 gcgcgcttgc cggcgccaag ccggaaaccc tctcctccag aaagaggcgc cgcatgctgt
     7261 ttcgcgacga cggctccgag gtcctcgccg tcagccaact cagccatgct tggatcgccg
     7321 ggcaaatcct ccgcgcttgg cgcgagaacc tgggcgagcg cctccttttt gcaaccgagt
     7381 tgcacgacat cggctggctc gactgggagg ccgccccaac gttcaatccg cagaccggcc
     7441 gcccccacct gttccgcgaa gtcgcccccg ccgttcacgc cccgatgtgg acgcaaggcg
     7501 tcgagcgcgc gctcgcggga tggggcgccc acgtggcgct gctcgtctca cgccatggcg
     7561 gcctgatcta ccggcgctac gcgcgccgcg gcgacggagc ggacgcggcg gcggtggaca
     7621 attacctggc cgcccaggcg gcctttgaag ggatttgggc cggcaagctc gggctgagtg
     7681 acgaggcgct gaacaaggaa acggcgctgc tcgccttcgc cgacgccttg tcgcttgcgc
     7741 tttgcggcga cctgaaagcg cctctcgacc tggaggcccc cggccatgac ggcgagacgg
     7801 tcagattccg gctggagcag ctcggccgcc ccttcgaatt taccctctcg ccgtggccgt
     7861 tccgcgaggc ggtcctgatt gtcgagggtg agggccggcc cctgccgacg ggcggccgat
     7921 ttcccagcga ggccgccatg cgcgagtggc ttgaggcgcc gcggcgcgcc agtttccaat
     7981 cgattttgcg ccgagcgtaa cgcgcggcgg gcgcgcatca gcgggcctga ccgccgatct
     8041 ttcggcgcgc tcccccgccc cgccgctttg cgctgaagct cgcaggcgcg gcttctcggc
     8101 cctgcagcca agcggcggga ggccttcttc cggtcagacg cgatccggct gaaggcggcg
     8161 atggagttcg aaaacgcctt gctcttccgc gggagcgccg gtcgaatcgg acggcttggc
     8221 cgggagttgg agcacccgcg catgctgtcc tgggcgaagc gaggtcaaca taacgatttc
     8281 gccgtccgga aactccaggg cgtcgtgatg aacgttgggc cggtgcatgt tcacctgccg
     8341 gaactgcgcc accttgcctc gggatttccg ccgccagaat cggcgcagga aggaaaaatc
     8401 gtatgaggtc agcacttcgt tgtcgaaagc gagctcggtt ccaggttgaa cgcagaccgc
     8461 cacatgggga gcgccaatgg cgcagaagcc gcgcgttgac gtcgccccga atcttgtcga
     8521 cactaatctg tcgccgatct ttgccggaac agacgcaaca tgatgaagac tatagtcgca
     8581 cattgctcaa acctccaatt tcttggatag actgcagagc cgtcggtccg atcgatattt
     8641 cggatggtgt gtaacgcgcg gagcagggga aagtttaagc ggccggctca tttataattg
     8701 agcttcgcgg cgccaatcgc ctcccgcctt acgatcgccc gaaatgatcg aacgggtagg
     8761 cgccgaagca gcggcgcatc cccgcggagg acgcccggat aggctggcgt gtgaaggtcc
     8821 tcgcgcgcca cgcagtattt ggagactcgt ttgagcgagc ccgaaccatc ttcgcccgct
     8881 tacgacgtga gcgaagccgc actcaaggag ctttatcgcg atctcgacgc agaccgcgcg
     8941 gatgtgatga agctcatcga cgaggccgag gcgctgatcg agtccaatct tgaacgcaag
     9001 ccaggctcaa acaagcgcga ggcgcgcgcc gccgcagata tcgcatcgaa gccggcggca
     9061 gattcgcttc cgaagccgac ggacgatgtc ggccacgacg cgctggttcg tccgcggcgg
     9121 cggcgaaggg ccggagctgg cttggcgctt ggcctcgttc ttctcttggt tggcttcgtt
     9181 gcttacgccc cgtttcgctc gcgactgacc tcggacgttc cgcgccgacc gccatcgatc
     9241 accggatttc tgcgcgtcgc cgaccttgcc gccggccata cgatcaagcc gggagacgtc
     9301 tatgcggtcg acatcccatc ggacgcggtg cgcatcaagg tgctatccgg acgcgtggcg
     9361 cttgtgacga agaacggcgc gtcgcttcgc gcttgctcgg accagccctt cgtgatcgaa
     9421 atcaaacatg gtgcgccgag cgccgccaat ccgcttcttt cgggctgcgg cggcgcgcct
     9481 tcgcggatcc aggccgtcat tccgcaaccg ccgctgcctt cgccggttcc tgctccggcg
     9541 atttcgcctg cgctgctcaa gcggcaggac cgcgactgac ggccggctca gcggcgaatt
     9601 ctcttctgcg cgcgttgcgc ggcagctttc gtacccaata attagggagt cggcggtcgc
     9661 cgagttttcg ggcggcggcg agcagtcggc agcgccattt tgatcaatcc ctcccggccg
     9721 ttggcggaac ca
//
LOCUS       DATGJW010000058         4959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_943896, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000058 DATGJW010000000
VERSION     DATGJW010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4959
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_943896"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..732)
                     /locus_tag="VKU03_01690"
     CDS             complement(<1..732)
                     /locus_tag="VKU03_01690"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HLJ69998.1"
                     /translation="MDVHARVGEVTERIVKRSAETRRRYLDQIEAYGAKTLPRKRLGC
                     ANFAHGFAACGPADKQALRDGDGPNLAIVTTYNDMLSAHQPFERFPEIIKRIAREAGG
                     TAQVAGGAPAMCDGVTQGEAGMELSLFSRDVIALSTAVALSHQMFDAAVYLGVCDKIV
                     PGLLIGALAFGHLPALFLPAGPMPSGLANDEKSKVRQLYAEGKVDRAALLEAEAKAYH
                     APGTCTFYGTANTNQMLMEIMGLHLP"
     gene            948..2639
                     /locus_tag="VKU03_01695"
     CDS             948..2639
                     /locus_tag="VKU03_01695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02231.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mucoidy inhibitor MuiA family protein"
                     /protein_id="HLJ69999.1"
                     /translation="MRSLVTTSALAGSSLLVFLSLAPAQAADIEAHSAVDAVTVYPDA
                     ALVTRTAQVDLPEGDSVLAFKDLPLTLDPASLRVAGAGDAKIAIGAVDSDVEPAAAKP
                     PDSALEAKLANLKTEREALRSKIDALQAKRAMILNYAKASPEKLTLDAKPLDISQWGA
                     AWDAVANGLFKLGDDLRPAEARAHALDDEIKALEAQRQRPSPATAPRRAANVALNAAA
                     PTHATITLSYRIAGANWVPAYDASLDTSGAKSVALVRRALLSQRTGEDWSNVSLTLST
                     ARVARSADAPDVRPIKIDFWQPPVYAPLESQRTLSAAPQAAQAPAPTAKKEMALAKPS
                     IAAEETASEGESGAYSAEFKVPGRVSLAADGAQKSFLLARASAPATIVAKTAPGLDQT
                     AYLEAHLTAAGDAPLLPGEVALHRDGMFVGRARIGFVAPGDGFDLGFGADDKIKVARA
                     PVNRKENEPTWYNQTKIETREFKTTVKNLHDFPVKLQVVDRLPYSENAAITAELLPAT
                     TPPTEKQVGDKRGVMSWTLDLQPGESKAITLAYRLKWPADRDIVIGAVTPAPAAR"
     gene            complement(2678..3829)
                     /locus_tag="VKU03_01700"
     CDS             complement(2678..3829)
                     /locus_tag="VKU03_01700"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HLJ70000.1"
                     /translation="MSETTRTQLPFLASLAGRRDPESRRILLRVATDRFVNDPAPSAE
                     ASARYEREALPLILDCDPATRLIVARKLAPLLAAPDAMLDVIVARGGDAALTVLERAV
                     AIDRDRLIAAIRGEVAEAAAVARRTDLDDELIRLIALRPEPAIGFALADNASAPIPTD
                     IFSLLARRAARDEKLARALLARPAAPLDQAPLFLYATSSQRAAMLLAAQRAELARSSA
                     AAAPWPAGAAAAVRLERQALARQPAQFVATLAEALGCDAELAERIVAEPLGEALAVAL
                     AALGAPEDATMRILLLGDAVSLDYKRLGALTRLKDALRPAAARRIISALVGRERLPGV
                     AAEPALDPAAAPTPSRPSPGRLGLPKADAAQVLRRRRAFALGGGMTRNG"
     gene            3981..4172
                     /locus_tag="VKU03_01705"
     CDS             3981..4172
                     /locus_tag="VKU03_01705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70001.1"
                     /translation="MLRPRSNAAPHPQAQQRPEGAQIVFFTGVRYQRIEPAPAPTAKR
                     APPKDGLNGAGRGKRKRRA"
     gene            complement(4280..4534)
                     /locus_tag="VKU03_01710"
     CDS             complement(4280..4534)
                     /locus_tag="VKU03_01710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HLJ70002.1"
                     /translation="MKKKFPEFKTDADAEKFVETADLSEYDLSDMIPMRFELKRKDKS
                     VSLRLPETLLDAVRQRARRSGMPYQRFIRMAIERALQHKI"
     gene            complement(4497..>4959)
                     /locus_tag="VKU03_01715"
     CDS             complement(4497..>4959)
                     /locus_tag="VKU03_01715"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HLJ70003.1"
                     /translation="FFSAPSRRVPPFKSQPNVRLRGLSGYRASAFLVLLLHMQNDMQV
                     AGGRERVEFDGFDWDDGNRGKCQKHGVSVAEIESLFRGSPLVGPDAKHSTVEGRYRAI
                     GATAQGRSLFIVFTWRQRGKALLLRPISARYMHRKEIKAHEKEIPRVQDRR"
BASE COUNT          863 a         1699 c         1630 g          767 t
ORIGIN      
        1 cggcagatgc aggcccataa tctccatcag catctggttg gtgttggccg tgccgtagaa
       61 ggtgcacgtg cccggcgcgt ggtacgcttt cgcctcggct tcgagcagcg cggcgcggtc
      121 gaccttgccc tccgcataga gctggcgaac cttcgatttc tcgtcgttgg caaggcccga
      181 cggcatcggc ccggccggca ggaacagagc cggcagatgg ccgaaggcga gcgcgccgat
      241 cagaagcccc ggcacgatct tgtcgcagac gccaagatag accgccgcgt cgaacatctg
      301 gtgcgacagc gcgaccgccg tcgacagcgc gatcacgtcg cgcgagaaga gcgacagctc
      361 catcccggct tcgccttgcg tgacgccgtc gcacatagcc ggcgcgccgc cggcgacctg
      421 cgccgtcccg cccgcctcgc gcgcgatcct cttgatgatt tccgggaagc gctcgaacgg
      481 ctgatgcgcc gacagcatgt cgttataagt cgtgacgatc gcgaggttcg gcccgtcgcc
      541 gtcgcgcaag gcctgcttgt cggccgggcc gcaggcggcg aagccatgcg cgaaattcgc
      601 gcagccgagg cgctttctcg gcaaggtctt cgcgccataa gcttcgatct gatcgagata
      661 gcgtcgccgg gtctcggcgc tgcgcttgac gatccgctcg gtgacttcgc cgacgcgcgc
      721 atgaacatcc atcgtcagcc tcgaataact tttgagccaa tgacggccgc cgcgtcctgc
      781 ggcagggcct tccgccgcct tttcgccgat agaagcgcga tcggcaagcg cgccgccttt
      841 tcatcggtcc cgcccaagcg tagccacgct tgcgccatcg cggcggccag ctttcgccca
      901 actgcccgtc gcaaatcgcc gcggcgataa tttcagaagg aaactccatg cgctcgctcg
      961 tcacgacctc ggctctcgcc ggatcgtcgc ttctcgtttt cctctccctc gccccggccc
     1021 aggccgccga catcgaggcg cattcggcgg tcgacgccgt gaccgtctat cccgacgccg
     1081 cattggtgac ccggaccgcc caagtcgacc tgccggaagg cgacagcgtt cttgccttca
     1141 aggacctccc cttgacgctc gatcccgcct cgctgcgggt cgcgggagcg ggcgacgcga
     1201 aaatcgcgat cggcgcggtc gattccgatg tcgagccggc cgccgccaag ccgccggaca
     1261 gcgcgctcga agcgaaattg gcgaacctca aaacggagcg cgaggcgcta cggtcgaaga
     1321 tcgacgcgct ccaggccaag cgcgcgatga tcctcaatta cgcgaaagcg tcgccggaaa
     1381 agctgacgct cgacgccaag ccgctcgata tttcgcaatg gggcgcggcc tgggacgcgg
     1441 tcgccaatgg gctcttcaag ctgggcgacg atctgcgccc ggcggaagcc cgggcgcatg
     1501 cgctcgacga cgagatcaag gcgctggagg cgcagcgcca gcgcccatcg ccggcgaccg
     1561 cgccgcgccg ggcggcgaat gtcgcgctca acgccgcggc gccgactcac gcgaccatca
     1621 ccctctccta tcgaatcgcc ggcgcaaatt gggttccggc atacgatgct tcgctcgaca
     1681 cgagcggcgc caaaagcgtc gcgctcgtcc ggcgcgcgct gctgtcgcag cgcaccggcg
     1741 aagattggag caacgtctcg ctcacccttt cgacggcgcg cgtcgcccgc tccgccgacg
     1801 cgcccgacgt gcggccgatc aagatcgact tctggcagcc gccggtctat gcgccgcttg
     1861 aatcgcagcg gacgctgagc gccgccccgc aagcggcgca agcgccggcg ccgacggcga
     1921 aaaaagagat ggctttggcc aagcccagca tcgccgccga ggaaaccgcc tctgaaggcg
     1981 agagcggcgc ctacagcgcc gagttcaaag ttcctgggcg ggtcagcctc gccgcggacg
     2041 gcgcgcagaa atccttcctg ctggcgcgcg cttcggcgcc ggcgacaatc gtcgcgaaaa
     2101 cggcgcccgg tctcgaccag acggcctatc tcgaagccca tctgaccgcc gccggcgacg
     2161 cgccgcttct gcccggcgag gtcgcgctgc atcgtgacgg catgttcgtc ggccgagccc
     2221 ggatcggctt tgtcgcgccc ggcgacggct tcgatctcgg cttcggcgcg gacgacaaga
     2281 tcaaagtcgc gcgcgcgccg gtcaaccgca aggagaacga gccgacctgg tacaatcaga
     2341 ccaagatcga gacgcgcgaa ttcaagacca cggtcaagaa cctgcacgac ttcccggtga
     2401 agctccaagt cgtcgatcgg cttccctatt cggagaacgc cgccattacc gccgagcttc
     2461 tgcccgccac cacgccgccg accgaaaagc aggtcggcga caagcgcggc gtgatgagct
     2521 ggacgctcga tctgcagccc ggcgagagca aggccataac tctcgcctac cgcctgaaat
     2581 ggcccgcgga ccgcgacatc gtgatcggcg cggtcacgcc ggcgccggcc gcgcgatgag
     2641 cgctgctgca gccgcgctcg gcgaccgcgg cgacagctca accgttgcgg gtcatgccgc
     2701 cgccgagggc gaaggcgcgg cggcggcgca ggacttgcgc ggcgtccgct ttgggcaggc
     2761 cgagccggcc cggcgacggc ctgctcggcg tcggcgcggc ggcaggatcg agcgccggct
     2821 cggcggcgac gcccggcaga cgctcccggc cgaccaaggc cgagatgatc cggcgcgccg
     2881 cggccgggcg caaggcgtcc ttcaggcggg ttagggcgcc gaggcgctta tagtcgaggc
     2941 tgaccgcatc gccgagcaac aggatgcgca tcgtcgcatc ctccggcgcg ccgagcgccg
     3001 cgagggcgac ggcgagggcc tcgccgagcg gctcggcgac gatgcgctcg gccagttcgg
     3061 cgtcacaacc cagcgcctcg gccaaggtgg cgacgaattg cgccggctgg cgcgccagcg
     3121 cctggcgctc gagccgaacc gccgccgcgg cgccggccgg ccagggcgcc gccgccgctg
     3181 aagagcgcgc caattcggcg cgctgcgccg cgagcagcat ggcggcgcgc tgcgaagagg
     3241 ttgcgtaaag gaagaggggc gcttgatcca agggcgcggc cggccgggcg aggagggccc
     3301 gcgccagctt ctcgtcgcgg gccgcccggc gcgccagcag cgagaaaata tccgtcggaa
     3361 tcggcgccga ggcgttgtcg gcaagcgcaa acccgatcgc cggttcgggc ctgagcgcga
     3421 tgaggcgaat cagctcgtcg tcgagatcgg ttcgcctggc gaccgccgcg gcttcggcga
     3481 cctcgccgcg aatcgccgcg atcagccggt cgcgatcgat ggcgacagcg cgttcaagca
     3541 cggtcagcgc ggcatcgccg ccgcgcgcga cgatcacgtc cagcatcgcg tcgggcgcgg
     3601 cgaggagcgg ggcgagcttt cgcgcgacga tcagccgggt cgccggatcg caatcgagga
     3661 tcagcggcaa ggcctcccgc tcgtatcgcg cgctcgcttc ggctgacggc gcgggatcgt
     3721 tgacgaatcg gtcggtcgcg acccgcaaca ggatgcgacg cgattcggga tcgcgacgac
     3781 cggccagcga ggcgaggaaa ggaagctgcg tgcgtgttgt ctcggacaca ggcgccaagc
     3841 tccgttaagg atgaacgcgt attgtcgagg ttaggccgat ctcgttgacc aatcgttaac
     3901 cttcacgccg gttaactgaa ttctcgaaac gccgcttggg gagcagcgct tcgggagaag
     3961 gggctatggg cgatttggtg atgcttcgac ctcggtcgaa cgccgcgccg catccgcagg
     4021 cgcagcagcg cccggaaggc gctcagatcg tgttcttcac cggcgtcagg tatcagcgca
     4081 tcgaaccagc gccggcgccg acggccaaac gggcgccgcc gaaggatgga ctcaacggcg
     4141 ctggacgcgg caagcgcaaa aggcgggctt gagccttcgc cgcgcgctgg cttcttacac
     4201 ggcgttaatc ccgacgaacc ttagcggcgc aacatcggac gcgcgcgacc gacgaagctc
     4261 catcgcgccc cggagatccc taaattttgt gttgcaaagc gcgctcgatc gccatgcgaa
     4321 taaagcgctg ataaggcatg cctgaacgtc gcgcgcgttg gcgcacggcg tcgaggagcg
     4381 tttcgggcag gcggaggctg acagatttat ctttccgctt caattcgaag cgcatgggaa
     4441 tcatgtcgga caagtcgtat tcacttaggt cggctgtttc gacgaacttt tcagcgtcag
     4501 cgtctgtctt gaactctggg aatttctttt tcatgggcct taatctcttt tctatgcatg
     4561 tagcgcgcgc taatcggccg cagcaaaaga gccttgccgc gctgtctcca tgtgaacacg
     4621 ataaataagc tgcggccctg cgccgtggcc ccgatcgccc ggtagcgacc ttcgacggtc
     4681 gagtgctttg cgtcggggcc cacaagcggc gagccacgga acaggctctc gatctcggct
     4741 accgagacgc cgtgcttctg gcacttgccg cgattgccgt cgtcccaatc gaagccgtca
     4801 aactccaccc tctccctccc tcctgcaacc tgcatatcat tttgcatatg caaaagcaat
     4861 actaaaaacg cgctggctcg gtagcccgaa agacctcgta accttacgtt aggctggctc
     4921 ttaaagggag gaactcttcg tgaaggcgcc gaaaagaac
//
LOCUS       DATGJW010000059         3636 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1358778, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000059 DATGJW010000000
VERSION     DATGJW010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3636)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3636)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3636
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1358778"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(36..557)
                     /locus_tag="VKU03_01720"
     CDS             complement(36..557)
                     /locus_tag="VKU03_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70004.1"
                     /translation="MAKLSRRGVMALIAMGAILASSTGGAETPPPLPPNTHFKAIRVD
                     VAPLLANSGEPTAGWMAQSLTGPLQAAFAAHLAPGDRAAPTLVVRIDNVFLGESGNGV
                     AGFTGDVTARDNIQGAGVVVGPDGRTVAVYPLFSVLYNYTGGTNFEMGSYRRRIAELA
                     QSFAQWLPGQMGL"
     gene            complement(596..1069)
                     /gene="greA"
                     /locus_tag="VKU03_01725"
     CDS             complement(596..1069)
                     /gene="greA"
                     /locus_tag="VKU03_01725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HLJ70005.1"
                     /translation="MEKFPITVAGHSKLFEEFQRLQQVERPRIIQAIAEARGHGDLSE
                     NAEYHAAKEAQSLNEGRIAELEDKLQRAEVIDVSKLSGTTVKFGATVTLVDEDTDEEK
                     ILQIVGETEADVKSGRISITSPTARALIGKKKGDTVEVNTPGGGKSYEILKVTFV"
     gene            complement(1182..>3636)
                     /gene="carB"
                     /locus_tag="VKU03_01730"
     CDS             complement(1182..>3636)
                     /gene="carB"
                     /locus_tag="VKU03_01730"
                     /EC_number="6.3.5.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase large subunit"
                     /protein_id="HLJ70006.1"
                     /translation="LPADPNAVRAALARTSPDRLRIVAEAMRAGFPRSEIAAITRYDR
                     WFLDEIANDITGGATPASFEPTIDYVVTKIPRFAFEKFPGAEPILTTSMKSVGEAMAI
                     GRTFAESLQKALRSLETGLTGLDEVEIEGLGKGDDHNVMRAAIGAPTPDRLLNVAQAM
                     RFGMSVDEIHDVCRIDPWFLERLAEIVALEEKVRKFGLPKDAANLRRLKAAGFSDVRL
                     ATLAGISETEVAAARRALGVVPVYKRIDTCAAEFASPTAYMYSTYEAPFGGKTVCEAR
                     PSAREKIVILGGGPNRIGQGIEFDYCCCHACFALSEAGYETIMINCNPETVSTDYDTS
                     DRLYFEPLTAEDVTAILDKEREQGKLKGVIVQFGGQTPLKLARAIEQAGAPILGTSVD
                     SIDLAEDRDLFKRLLDKLGLKQPKNGIAYSVEQSRLVAADLGLPLVVRPSYVLGGRAM
                     AIIRDQSDLDDYLLGTLPGLVPSEIKARYPNDKTGQINTVLGKNPLLFDRYLSDAVEV
                     DVDALADGDQAVVAGVMEYIEEAGIHSGDSACSLPPRSLGAETVEKLADQTRKLALAL
                     KVGGLMNVQFALKDGEIYVLEVNPRASRTVPFVAKVVGRPIAKIAARVMAGEKLSAFA
                     LKFGALGHVGVKEAVFPFARFPGVDTVLGPEMRSTGEVIGLDRSFEVAFAKSQLGAGA
                     AAPRSGTVFVSVRDADKPRILATMELLAGLGFNIVATGGTQRFLVAAGVPAQKINKVS
                     EGRPHCVDAIKNGSIQLVFNTTEGAQALADSRSLRRAALLHKVPYYTTLAGAIAAAQG
                     IKAYIGGDLEVRALQDYFS"
BASE COUNT          612 a         1184 c         1195 g          645 t
ORIGIN      
        1 tggattgctt cgcttcgctc gcaatgacgg gcgcctcaaa ggcccatctg gccgggcagc
       61 cattgcgcga agctttgcgc aagctcggcg atgcgccggc gataggaccc catctcgaaa
      121 ttcgtcccgc cggtgtaatt gtagagcacg ctgaacagcg gatagaccgc caccgtgcgg
      181 ccgtccggtc cgacgacgac gcccgcgccc tggatgttgt cgcgggcggt gacgtcgccg
      241 gtgaagcccg ctacgccatt gccggattcg ccgaggaaga cgttgtcgat cctcacgacc
      301 agggtcggcg cggcgcgatc gccgggcgcg agatgagcgg cgaaggccgc ctgcagcgga
      361 ccggtcaggg attgcgccat ccagcccgcg gtcggctcgc ccgaattggc gaggagcggc
      421 gcgacatcga cgcgaatggc tttgaaatgg gtgttgggcg gcagaggcgg cggcgtttcc
      481 gcgccgccgg tcgaactcgc caagatcgcg cccatggcga tgagcgccat cacaccgcgc
      541 cgggaaagct tcgccatcgc cttctccttc gtctctcccc ggccggcagg cccgatcaca
      601 cgaaagtgac tttcaggatt tcatagcttt tgccgccgcc gggcgtgttc acttcaaccg
      661 tgtcgccctt cttcttgccg atcaaagcgc gggccgtcgg cgaggtgatc gagatgcggc
      721 ccgacttcac gtcggcctcg gtctcgccga cgatctgcag gattttctcc tcgtcggtgt
      781 cctcgtcgac cagcgtcacg gtggcgccga acttgacggt ggtgccggaa agcttcgaca
      841 cgtcgatgac ctcggcgcgc tgcagcttgt cttcgagctc ggcgatgcgc ccttcattga
      901 gcgattgcgc ctccttggcg gcgtggtatt cggcgttctc cgaaaggtcg ccatggccgc
      961 gcgcttcggc gatcgcctgg atgatgcgcg ggcgctcgac ttgctgcagg cgctggaatt
     1021 cctcgaaaag cttggaatgg ccagcgacgg tgatcggaaa cttctccatc ctcaaccttg
     1081 ccttagatct gttgcgttct atttacgcga cttcagcgta tatcctgcgc cctcccgatg
     1141 gcaggctcgc tttggcgcgg aaagcctcgc gaatcgatcg ctcaggaaaa ataatcctgc
     1201 aacgccctca cttcgaggtc gccgccgata taagccttga ttccctgagc cgccgcgatg
     1261 gcgcctgcta gagtggtgta ataggggacc ttatgcaaga gggcggcccg gcgcagcgag
     1321 cgtgaatcgg cgagcgcctg cgccccctcg gtcgtgttga aaaccagctg aatcgagccg
     1381 tttttaatgg cgtcgacgca atgcgggcgc ccctccgaca ctttgttgat cttctgggcc
     1441 ggaacgcccg cggcgacgag aaagcgctgg gtgccgccgg tcgcgacgat gttgaagccg
     1501 aggccggcga gcagctccat cgtcgcaagg atgcgcggct tgtcggcgtc gcgcaccgag
     1561 acgaagaccg tcccggagcg cggcgccgcc gccccggcgc cgagttggct cttggcgaag
     1621 gccacttcaa acgaccggtc aaggccgatc acctcgccgg tcgagcgcat ttccggtccg
     1681 agcaccgtat cgacgccggg aaagcgggcg aaggggaaca ccgcctcctt gacgccgaca
     1741 tgaccgagcg cgccgaactt caaagcgaag gcggaaagct tctcgccggc cataacccgc
     1801 gccgcgatct tggcgatcgg ccggccgacg accttggcga cgaagggcac ggtgcgcgag
     1861 gcgcgcgggt tgacctccaa cacgtagatt tcgccgtctt tcagcgcgaa ttgcacgttc
     1921 atcaggccgc cgaccttgag cgccaaggcg agcttgcggg tctggtcggc gagcttctcg
     1981 accgtctcgg cgccgagcga gcgcggcggc aacgagcagg cgctgtcgcc ggaatgaatg
     2041 ccggcctcct cgatatactc catcaccccg gcgacgaccg cctgatcgcc atcggcgagc
     2101 gcgtcgacgt cgacctccac cgcgtccgac agatagcggt cgaagagcag cggattcttg
     2161 ccaagcaccg tgttgatctg gccggtcttg tcgttcggat agcgcgcttt gatctcggac
     2221 ggcacgaggc ccggcagcgt gccgagcagg taatcgtcga ggtcgctttg gtcgcgaatg
     2281 atcgccatgg cccggccgcc gagcacatag gacgggcgca ccactagcgg caggccgaga
     2341 tcggcggcga cgagccgcga ctgctcgacc gaataggcga tgccgttttt cggctgcttg
     2401 aggccgagct tgtcgagcag gcgcttgaac aaatcgcgat cctcggcgag gtcgatcgaa
     2461 tcgaccgacg tgccgaggat cggcgcgccg gcctgctcga tcgcccgcgc cagcttcagc
     2521 ggcgtctggc cgccgaactg gacgatgacg cccttgagct tgccctgctc gcgctccttg
     2581 tcgagaatcg cggtcacgtc ctcggcggtg agcggctcga aatagagccg atcggacgta
     2641 tcgtaatccg tcgatacggt ctccggattg caattgatca tgatcgtctc gtacccggcc
     2701 tcgctcaaag cgaagcaggc gtgacagcag caatagtcga attcgatgcc ttggccgatg
     2761 cggttcgggc cgccgccgag aatgacgatc ttctcgcgcg ccgacggccg cgcctcgcaa
     2821 accgtcttgc cgccgaacgg cgcttcgtag gtcgaataca tgtaggcggt cggcgaggcg
     2881 aattccgccg cgcaggtgtc gatgcgctta tagaccggga cgacgccgag ggcgcgccgc
     2941 gccgcggcga cttccgtttc ggaaattccg gcgagcgtcg ccaggcgcac atcggaaaag
     3001 cccgccgcct tgaggcggcg gagattggcc gcgtccttcg gcaggccgaa tttgcgcacc
     3061 ttctcttcga gcgcgacgat ttccgccaaa cgctcaagga accacggatc gatccggcag
     3121 acgtcgtgaa tctcgtcgac gctcatgccg aagcgcatgg cctgggcgac attgagcagc
     3181 cgatcgggcg tcggcgcgcc gatcgccgcg cgcatcacgt tgtgatcgtc gcccttgcca
     3241 agcccttcga tctcgacctc gtcgaggccg gtcaatcccg tctccagcga gcgcagcgcc
     3301 ttttggagcg actcggcgaa ggtgcggccg atcgccatcg cctcgccgac cgacttcatc
     3361 gacgtcgtga ggatcggctc ggcgccgggg aacttctcga aggcgaagcg cgggattttg
     3421 gtgacgacat aatcgatcgt cggctcgaac gaagccggcg tcgcgccgcc ggtaatgtca
     3481 ttggcgatct cgtcgaggaa ccagcggtca tagcgcgtga tggccgcgat ttcgctgcgc
     3541 ggaaaaccgg cgcgcatggc ttcggccacg atccgcaggc gatccgggct cgtgcgagcg
     3601 agcgccgcgc gcaccgcgtt ggggtcggcc ggaagg
//
LOCUS       DATGJW010000060         6025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_736491, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000060 DATGJW010000000
VERSION     DATGJW010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6025
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736491"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..463
                     /locus_tag="VKU03_01735"
     CDS             <1..463
                     /locus_tag="VKU03_01735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HLJ70007.1"
                     /translation="IRRRDGDPIIAFADLHRQPGDRPDIETVLAPDELIAAFRLPAAP
                     WTRRSLFLKIRDRESYEFALASAAVALDLDQGGVVREARIALGGVATTPWRARRAEAA
                     LAGKTLDDEAAQQAAEIAFRDAAPRKHNAFKIALGRQTLTRALLETAALEI"
     gene            465..2738
                     /locus_tag="VKU03_01740"
     CDS             465..2738
                     /locus_tag="VKU03_01740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLJ70008.1"
                     /translation="MATAPANNMGKPLPRLDGRLKVTGAARYPSDAAVANPAFACLVT
                     SSIARGVIRAIRLDAARAVPGLLDILTFQNANEIRPLKTFSEGGQAASSIAPLGAPKI
                     WHDGQIVALVVAETFEAASEAAQKVIVEYDEERPTPTLGAPGAEVQAVADVSKAHKDP
                     GFGDAAAAYEAAPVKIDAEYSTPTQHHNPMELFSTTCLWADGKLTIHEPSQFVYGVKN
                     GVATQLGMNPDDIRVESRYIGGAFGSKGSLTARTAIVALAAKRLGRPVKLVAARDQGF
                     TIATYRAETLHKVNLGADRGGRLLAYLHEAFELTSRPDNYNVSGTTSTAVMYNYGAVA
                     TKVNIVHADRNTPGFMRSPPEVPYMYALESAIDELAYALKMDPVELRRVNDTMKDPAD
                     GRPYTSRSLMQCFDRAAAAFGWDRRSPQPGSMRDGDWLIGWGCATACYPTQIAPAAAR
                     VSLARDGSARVQIAAHEIGTGAYTVIGQAAADRLGLDIERVTVELGDTALPPGPVAGG
                     SNTTASATSAVVKACDAIREKLFRAAIASNESPLAGKPVAALDLAGGRIVAGEASEAL
                     EAAFKVLGQATIEEYVESIPLSLSPEAMEKLYQGRVALAGGPKGDRLMFAFGAEFVEA
                     RVHARTREIRVPRAVGAFAAGHIMNPRTAHSQLMGGMIWGVSSALHEATEIDERTARY
                     VNDNLADYLIPVNADVQTVDVLFVPEIDRDINPLGVKGLGELGNVGTNAAVANAVYHA
                     TGVRVRDLPIRIEKLLV"
     gene            2754..3194
                     /locus_tag="VKU03_01745"
     CDS             2754..3194
                     /locus_tag="VKU03_01745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPC domain-containing DNA-binding protein"
                     /protein_id="HLJ70009.1"
                     /translation="MKSKLVAGVDGRSFVLALDPGEEAFAAITKFAQAEGLPGASLTA
                     IGAFERATVGWFDIAKKEYRKIEIAEQCEALSLVGDVAIDDGGAPSLHLHAVLGLSDG
                     STRGGHLLEAIVQPTLEVMIRETPKGFRRKKRPDLGLALIDLSQ"
     gene            3298..3435
                     /locus_tag="VKU03_01750"
     CDS             3298..3435
                     /locus_tag="VKU03_01750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70010.1"
                     /translation="MEERPVLKPTEARQGVTGHNVRYVVVIGVALVIIAFIAVAAFVG
                     R"
     gene            3530..5734
                     /locus_tag="VKU03_01755"
     CDS             3530..5734
                     /locus_tag="VKU03_01755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLJ70011.1"
                     /translation="MAVSPAWAAGAPAPSEGKFLAALVVILLASRLLGEAMQRLGQPA
                     VIGHLIAGILLGPSFFGWVWPAGQHVVIPSDSTDKAMLAGLAQFGVLLLLLLTGMDTD
                     IALIRKVGRPAISVSLTGIAVPFVCGVAAAYFLPAALLPHAEQRLVMALFLGVALSIS
                     SIKIVAMVVHEMNFMRRDLGQIILASSIIDDSIGWIIIAIVFGIARAGAIQVEPLLES
                     VIGVAAFLAISLTVGRRAVAFAIRFVNDNFTSELPVLTLILLIMGTMALITAALGVQS
                     VLGAFVAGLLIGESPILTKHLTIQLRGMIVAFFAPVFFAIAGLSADLTVLTAPELAAL
                     TLGLVLIASVGKFAGAFLGGAIGRLSRRESLALAIGMNARGSTEVIVASIGLSIGALS
                     QNLYTMIVTMAVLTTCAMPPTLRWALSRVPLRPGEKERLERESYEAKGFVANMERLLL
                     AASDDANGRFASRLAGLLAGPRGLPTTVLHVEGEPALGQEKMIVAERADEMAANVRQS
                     AKAARHQPNGETDAPAVAVKTRAEAALPARAVSNEAPKGYDLLIIGLDPARMPEGGFN
                     PEIARTARAYDGPLAVVVARGAHSEDPIASRLTLLVPVTGAPASKRAAEIGIELARAA
                     HAELTILHVAAAQSTANATSRRRQRMIARRHEEAVLREIVEIADRYDVRARTKITTSA
                     NPAHAILSEAERARDTLIVLGVATRPSDALLFGATADQLLEASRCSLLFVAS"
BASE COUNT         1004 a         2043 c         1925 g         1053 t
ORIGIN      
        1 aatccggcgg cgggacggcg acccgatcat cgctttcgcc gatcttcatc gtcagcctgg
       61 cgaccggcca gacatcgaga cggttctggc gcctgacgag ttgatcgccg cctttcgcct
      121 tcccgcggcg ccatggacgc gccgctcgct tttcctgaaa atccgcgatc gagagtctta
      181 cgaatttgcg ctggcgtcgg ccgccgtagc gctcgatttg gaccaaggcg gcgtcgtgcg
      241 cgaagcgcgc atcgcgctgg gcggcgtcgc cacgacgccc tggcgcgctc gccgcgccga
      301 agccgctctt gccggcaaga ctctggatga cgaggcggcg caacaggcgg cggaaatcgc
      361 cttccgggac gccgcgccgc ggaagcacaa cgccttcaag atcgcgctcg ggcgacagac
      421 cttgacgcgc gcgcttctcg aaaccgccgc gttggagatt tgagatggcc accgcgcccg
      481 cgaacaatat gggcaagccc ctgccccggc tcgacggccg gctcaaagtc accggcgctg
      541 cgcgttatcc ttccgacgcg gcggtcgcca atcccgcttt cgcctgcctc gtcaccagca
      601 gcatagcccg cggcgtcatt cgcgccatcc ggctcgacgc cgcccgcgcc gttcccggcc
      661 tgctcgacat tctgactttc cagaacgcga acgagatccg gccgctgaag accttctcgg
      721 agggcggcca ggcggcgagc tcgatcgcac cgctcggcgc gccgaagatc tggcatgacg
      781 gccagatcgt cgccctggtc gtcgccgaga ctttcgaagc ggccagcgaa gcggcgcaaa
      841 aagtcatcgt ggagtacgac gaagagcggc cgacgccgac gcttggcgcg cccggcgcgg
      901 aggtccaagc cgtcgctgat gtcagcaagg cgcacaaaga cccagggttc ggcgacgccg
      961 ccgccgcgta cgaggccgcg ccggtcaaga tcgacgccga atattcgacg ccgacgcagc
     1021 atcacaatcc gatggagctt ttttccacga cctgcctttg ggccgacggc aagctgacca
     1081 tccatgagcc gagccaattc gtctatggcg tcaagaatgg cgtcgcgacg cagctcggca
     1141 tgaatcccga cgatatccgc gtcgagagcc gctatatcgg cggcgcgttc ggctcgaagg
     1201 gctcgctcac cgcgcgcacg gcgatcgtcg cgctcgcggc gaaacggctc ggccggccgg
     1261 tcaagttggt cgcggcgcgc gatcagggct tcaccatcgc gacgtaccgc gccgagacgc
     1321 ttcataaggt taatctcggc gccgatcgcg gcggaaggct tctcgcctat ttgcatgaag
     1381 cgttcgagct cacgtcgcga cctgacaatt ataacgtttc cggcacgacg agcacggccg
     1441 tgatgtacaa ttacggggcg gtcgcgacga aggtgaacat cgtccatgcc gatcgcaaca
     1501 cgcccggctt catgcgctcg ccgccggaag tgccgtatat gtacgcgctc gaaagcgcga
     1561 tcgacgagct ggcctatgcg ctgaagatgg acccggtcga gttgcgccgc gtcaacgaca
     1621 cgatgaagga ccccgccgac ggccggcctt acaccagccg ctcgctcatg caatgtttcg
     1681 atcgagccgc ggcggctttc ggctgggacc gtcgatcgcc gcagccaggc tcgatgcgcg
     1741 acggcgactg gctgatcggc tggggctgcg cgacggcctg ctacccgacc cagatcgcgc
     1801 cggccgcggc gcgggtcagc ctggcgcgcg acggatcggc gcgcgtgcag atcgccgcgc
     1861 acgagatcgg cacgggcgcg tatacggtga tcggccaggc cgccgccgac cgcctgggcc
     1921 tcgacatcga gcgcgtgacg gtcgaactcg gcgacaccgc cttgccgccc gggccggtgg
     1981 ccggcggctc aaacacgacg gcgagcgcga cctccgccgt ggtcaaggct tgcgacgcca
     2041 ttcgcgaaaa gctctttcgc gccgcgatcg cctcgaacga gagtcccttg gccggaaagc
     2101 ccgtcgcggc gctcgacctt gccggcgggc ggatcgtcgc cggcgaggcc tccgaggcgc
     2161 ttgaagcggc tttcaaagtc ttggggcaag cgacgatcga ggaatatgtc gaatcgattc
     2221 cgctgtcgct ctcgcccgag gcgatggaga agctctatca gggtcgcgtc gccttggccg
     2281 gcggtccgaa aggcgatcga ttgatgttcg ctttcggcgc cgagttcgtc gaagcgcgcg
     2341 ttcatgcgcg aacacgggaa attcgcgtgc cgcgcgctgt cggcgcgttc gcggcgggcc
     2401 acatcatgaa tccccgcacc gcgcatagcc aattgatggg cggcatgatt tggggagtga
     2461 gctcggcctt gcacgaggcg acggaaatcg acgagaggac cgcgcgctat gtcaacgaca
     2521 acttggcgga ctatctcatc ccggtgaatg cggacgtcca aaccgtcgac gtcctcttcg
     2581 ttccggagat cgaccgcgac atcaatccgc tcggcgtcaa gggactcggc gaactgggca
     2641 atgtcgggac gaacgccgcc gtcgccaacg ccgtctatca cgcgacgggc gtgcgggtgc
     2701 gcgatctgcc gatacggatc gagaagctgc tcgtctaaat cggaggagag gcgatgaaat
     2761 cgaaactggt cgccggcgtc gacggccgca gcttcgttct ggcgctcgat ccaggcgaag
     2821 aggctttcgc cgcaatcacc aaattcgccc aggcggaagg gctgcccggc gcgtcgctga
     2881 cggccatcgg cgccttcgaa cgcgcgacgg tcggatggtt tgacatcgcc aagaaagaat
     2941 atcgaaaaat cgagattgca gagcaatgcg aagcgctgag cctggtcggc gatgtcgcga
     3001 tcgacgacgg cggcgcgccg agcctccatc tccacgctgt gctcgggctc agcgacggct
     3061 cgacccgcgg cggccacttg ttggaggcca tcgtccagcc gacgctcgaa gtgatgatca
     3121 gggaaacgcc gaaggggttt cggcgcaaga agcggccgga tctcggcctc gcgctcatcg
     3181 atctctcgca ataacgcaaa gagcgcctct gcgccgatct cggccgcctg gaacgcggcc
     3241 gcgggctcgc gcgtttacca aaaaggaggc gtcaattcgt cgccagcgga gctcgccatg
     3301 gaagaacggc ctgttctgaa gcccaccgag gcgcggcaag gcgtgaccgg ccacaatgtt
     3361 cgctatgttg ttgttatagg cgtcgcgctg gttatcatcg ctttcattgc ggtcgcggcg
     3421 ttcgtcggcc gataaacagg cggagccgcc gccgacgtct cagggggcgc tgttgaaatt
     3481 tccctcagca gcccgcttcg ccggatgcgc cggagcggct ttgctttgca tggcggtttc
     3541 gccggcctgg gccgccggcg cgcccgcgcc gtccgaaggc aaattcctcg cggcgctcgt
     3601 cgtcatcctt ctcgcctcgc ggctgctcgg cgaagcgatg cagcgccttg gccagcccgc
     3661 tgtgatcggc cacctcatcg ccggcatcct gcttggcccg tctttcttcg gttgggtctg
     3721 gccggccggc cagcacgtcg tcattccgtc ggactcgacg gataaggcga tgctcgccgg
     3781 cctcgcgcaa ttcggcgtgc tgcttctgct gctcctgacg ggcatggata ccgacatcgc
     3841 gctcatccgc aaagtcggcc gtccggcgat cagcgtctcg ttgactggaa tcgccgtgcc
     3901 gttcgtctgc ggcgtcgcgg cggcctattt tctccccgcg gcgctgcttc cgcatgccga
     3961 gcaaagactc gtcatggcgc tctttctcgg cgtcgccctg tcgatctcgt cgatcaagat
     4021 cgtcgcaatg gtcgtgcatg aaatgaattt catgcgccgc gatctcggcc agatcatcct
     4081 cgcctcctcg atcatcgacg attcgatcgg ctggatcatc atcgcgatcg ttttcggcat
     4141 tgcgcgcgcc ggcgccattc aagtcgagcc gctccttgaa agcgtcatcg gcgtcgcggc
     4201 gttcttggcg atcagtttga ccgtgggccg gcgcgccgtc gccttcgcca ttcgcttcgt
     4261 caatgacaat ttcaccagcg agctgccggt cctgacgttg attctcctga tcatggggac
     4321 aatggccctg attaccgccg cgctcggcgt tcagagcgtg ctcggcgcct tcgtcgccgg
     4381 cctgctgatc ggcgaatcgc cgatcctcac caagcatctc accatccaat tgcgcggcat
     4441 gatcgtggcg ttcttcgcgc cggtcttttt cgcgattgcc ggcctcagcg ccgatctcac
     4501 cgtgctgacg gcccccgaac tcgccgcgtt gaccctcggg cttgtcctca tcgccagcgt
     4561 cggcaaattc gccggcgctt ttctcggcgg cgcgatcggc cgattgtccc gtcgcgaatc
     4621 gctggcgctc gccatcggca tgaacgcccg cggctcgacc gaggtcatcg tcgccagcat
     4681 cggcctgtcg atcggggccc tcagccaaaa tctctatacg atgatcgtca ccatggcggt
     4741 tctaacgacc tgcgcgatgc ctccgactct gcgctgggcg ctgtcgcggg ttcccctacg
     4801 cccgggcgag aaagagcggc tcgagcgcga atcgtacgaa gcgaaaggtt tcgtcgccaa
     4861 tatggagcgg ctgctgctcg cggcgagcga cgatgcgaat ggccgcttcg cctcgcgcct
     4921 agccggcctt ctcgccgggc cgcgcggcct gccgaccacc gtcctgcatg tcgaaggcga
     4981 gccggcgttg ggccaagaga agatgatcgt cgccgaaagg gccgacgaga tggccgccaa
     5041 tgtcaggcaa agcgcaaaag cggcgcgcca tcagccgaac ggcgaaaccg acgcgccggc
     5101 cgtcgccgta aagacgcgcg ccgaggcggc gctcccggcg cgcgccgtct ccaacgaagc
     5161 gccgaaaggc tacgatctgc tgatcatcgg cctcgatccg gcgcgcatgc cggaaggcgg
     5221 cttcaatccc gaaattgcgc gcacggcgcg cgcctatgac ggcccgctgg cggtagtcgt
     5281 cgcgcgcggc gcccatagcg aagatccgat cgccagccgg ctgactttgc tcgtgcccgt
     5341 caccggcgcg cctgcgtcaa aacgcgccgc cgaaatcggc atcgagcttg ctcgggcggc
     5401 ccatgccgag ctcacgatcc ttcacgtcgc cgccgctcaa tcgaccgcga acgcgacatc
     5461 gcgacgcagg caacgcatga tcgcgcgccg ccacgaggag gcggtcctcc gcgaaattgt
     5521 cgagatcgcc gaccgctacg acgtgcgcgc cagaaccaag atcacaacgt ccgccaatcc
     5581 cgcccatgcg attctgagcg aggcggagcg cgcgcgcgat acgctgatcg tgcttggcgt
     5641 cgccacccgg ccaagcgacg ctctgctctt cggcgccacc gccgatcaac tgttggaggc
     5701 gagccggtgc tcgctgctgt tcgtcgccag ttgagcgggc cgcaatatcg ccccaatagc
     5761 cgaaagcgcg ccgaattggt cgtgcgacaa gcgcgccggg cgcgagcagg aacaaaggaa
     5821 gcgccatcgc ggttcgcttt cagttagggt cttttcggat atggcgcggc tctgtcgctt
     5881 ttcgccgccg cgttgattcc gatcgccttc ctgctgcaaa aggtcgatct tggatcggcc
     5941 ccgagaggac actgagcgcg cccgccgcgc tctctttcga gatcagcttt tgctggcctg
     6001 ccactggcct tgatacttga attct
//
LOCUS       DATGJW010000061         8268 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_736728, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000061 DATGJW010000000
VERSION     DATGJW010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8268)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8268)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8268
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_736728"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            371..697
                     /locus_tag="VKU03_01760"
     CDS             371..697
                     /locus_tag="VKU03_01760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HLJ70012.1"
                     /translation="MIARLWRGYTRPEHAEAYEGMLKPELLPGVGKIPGYKGSYLLRR
                     EVGGEVEFVTVMLWDSIDAIRAVAGADYEKAVVPEERRPYLKRYDAASAHYDIVATHA
                     APSGEK"
     gene            complement(863..1831)
                     /locus_tag="VKU03_01765"
     CDS             complement(863..1831)
                     /locus_tag="VKU03_01765"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLJ70013.1"
                     /translation="MELSSEEDRSFATRLMEHLVVPTFVIDASGRVMIWNKACERLTG
                     VSADEIIGKSDHWRAFYGEPRPCLADLVLAGRYKDIKDLYASYNNVGFSDYGVSAENW
                     CVMPTIGHRLYLAIDAGPIYDGSGRLTAVVETLRDITVQKQAQMELETLATRDGLTGL
                     LNRRSFDERLATEARRAARERQPLSLLMLDIDCFKQYNDAYGHQKGDECLRLVAGALT
                     KGLWRAGDAAARYGGEEFAVILPDTTRQGAASVAERMRREVIRLDIPHQASIVSDHVT
                     VSIGGSTIVAATISPDALLTSADAALYQAKHDGRNRSVFPEFTEAA"
     gene            complement(1957..2412)
                     /locus_tag="VKU03_01770"
     CDS             complement(1957..2412)
                     /locus_tag="VKU03_01770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70014.1"
                     /translation="MRSLVLGAGFLAVLAGAAPARQATAGEAYCPNPAHAGPETAPPR
                     MAAAIARALHIDEAAARNAFVRCVGAKLMACTVGANLDCFKADTRRSLPGATAWCRDN
                     PDADNIPMVATGHATIYAWSCQGGRAVAGKRLVAVDPQGFVANNWREVR"
     gene            complement(2433..3068)
                     /locus_tag="VKU03_01775"
     CDS             complement(2433..3068)
                     /locus_tag="VKU03_01775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HLJ70015.1"
                     /translation="MIHPANLAAFGAVALGLVLTPGPNMAYLVSRSVCQGRAAGLVSL
                     AGVALGFIVYVLFAAFGVTALLFAIPYAYDGLRWAGAAYLAYLAWGALKPGGASPFQL
                     RELKADSPRRLFGMGLLTSLLNPKIAALYLSLLPQFVDRGSGGILGQTLTLGALQIAI
                     SVTFNAIFVFAAGTIAAFLAGRPLWARVQRWIMGGVLAALAARLALDSGRR"
     gene            complement(3114..4919)
                     /locus_tag="VKU03_01780"
     CDS             complement(3114..4919)
                     /locus_tag="VKU03_01780"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008123004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLJ70016.1"
                     /translation="MNRRALLQIAAGLPFASALWRSALTPAQAAPTARVRPGDPGWPS
                     DESWEQLNSEVGGRLIKVRSPLDACFEAPSGAACAQVFKELKNPYYLGDEAALTQTLG
                     WVDAWTSRPSVYAVAAETTSDIVAAVNFARKYNLRLVVKGGGHSYQGQSNAADSLLIW
                     TRKMNTVALHDAFVGVGCAGRSEPAPAATIGAGAIWGHTYNQATTKSGRYVQGGGCMT
                     VGVAGLILGGGFGSFSKAFGTAAASLLEAEIVTADGEVKLANSCTNPDLFWALKGGGG
                     ALGVVTRVTVRTHDLPQIFGAVFATIKAGSDDAYRRLIDKIVALYRDKLFNSHWGEQL
                     VFRPGGVLAVAMVFQGLEQSEAEAAWRPFFDWIKAAPQDFTMQAGPQVLAVPARHFWD
                     PAALKSVPGLVIADDRQGAPADNIFWAGNLGEAGEVLHAYQSLWLPASLLDDDRRQSL
                     VDALFAAAQHWGVSLHFNKGLAGASPETIDSAKDTAMNPAVVDAFALAISGAHEQPAY
                     PGVAGHEPDAAAARSDAASVTKAMGELRKLVPRAASYIWESDFFEPNWQESYWGGHYA
                     RLRAVKEKYDPHGLFYIHHGVGSESWSADGFTKHG"
     gene            5075..5755
                     /locus_tag="VKU03_01785"
     CDS             5075..5755
                     /locus_tag="VKU03_01785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLJ70017.1"
                     /translation="MTQSSQTLARAPLFASLGEADIRALDSRCVWRKVKAGAWVIDDH
                     ADGADVFFVVDGHARVVIAGSKRDIILRDILDGEYFGELSAIDGKPRSAGIVAITDTV
                     VAQMSAKTFREMIHQHPSVCDQVLERLAASIRAVNDRVNEQVNFEARDRLILELLRLS
                     REREGGRVVVSPPPTHAEIAARIGAHREAVTKFLNALEREGAISRTRGAIVLTDPELL
                     RNMVDRAA"
     gene            complement(5871..6125)
                     /locus_tag="VKU03_01790"
     CDS             complement(5871..6125)
                     /locus_tag="VKU03_01790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70018.1"
                     /translation="MRSLLFIGLLAVALGYSDGRALAASNPNAGSTSVAAVEQNEGTN
                     ESFAAEAGKIAILAGAGLIGTLAAVGLRRIWLRNPIHDPY"
     gene            6355..6879
                     /locus_tag="VKU03_01795"
     CDS             6355..6879
                     /locus_tag="VKU03_01795"
                     /inference="COORDINATES: protein motif:HMM:NF021538.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2269 family protein"
                     /protein_id="HLJ70019.1"
                     /translation="MVRLLKICHLLGLTLFLGSILGHIVASVAAGAPGGESFLYAREH
                     IALATHFLTLPGLALSIVSGIALVALSRLSPLRHRWLAAKIGLTLVVMVNSTLFLIPA
                     GARALAGAVALSRGDADAMAEIVAALRVEQHAGAANILLILLIVVLGVFKPRLQSRRR
                     AAGKVLSSSGAASR"
     gene            6893..7363
                     /locus_tag="VKU03_01800"
     CDS             6893..7363
                     /locus_tag="VKU03_01800"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ70020.1"
                     /translation="MAKKAGRAAPWRYRREESLGYLVNYLGRVLARALERRLVKHGVT
                     LGQFPLLLTLWEEESLTQSEIARRLDIEQPTVANTLRRMERDGLVKAEPDPSHRRQVL
                     IKLTEKGRKLERPLKLEALAVNQAAAAKISASDLEAFRRTIRLLLETLEGRTDA"
     gene            complement(7463..>8268)
                     /locus_tag="VKU03_01805"
     CDS             complement(7463..>8268)
                     /locus_tag="VKU03_01805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HLJ70021.1"
                     /translation="GLVPGQHFENVDPEDDPRYRAYVASYLDVAGRKGVTPSAAKTVV
                     RTNTTVIGALAVRRGEADALICGLDGRFQTRLRLIKDIIGLAPGFEDCAALSLVIAQS
                     GAFFFADTHVRYDPSAEEVADVAIASASHVRRFGVTPKIALISHSDFGSADTPSALKM
                     RKALDILRQRAPDLEVDGEMQADTALSIVARERVLTGSRLNGEANVLIFPNLDTGNTA
                     LQLTRVIAGALPVGPILIGPAKPAHILTPSVTARGIVNITAIAAVEAGG"
BASE COUNT         1506 a         2593 c         2751 g         1418 t
ORIGIN      
        1 gcgttctatt ttcagatcgg ctggcgacgc ggggccgaca tcgtgcaaga ggggcagacg
       61 gattcgggtc tcgcctcgcg atggagcttc accgagtcac gccgcgctcg ttccgctggc
      121 tcggcgaatt ttcttccgac aagggcgcgt cctggcggct cgcggttgaa gttctcgctc
      181 gtcggatttc gccggtggat caggattgca aaataccggc ggaacaggat gcgagcgttt
      241 agcggctcgg tccgccgtca ttgcgaggcg cgacgaagct ccatctaacc ctagctctac
      301 gaaggaattt tttcgcgacg ctcgcggcgt ggcgcgactc gcgaaattca agcgagaaag
      361 gcgacgactc atgatcgcaa gactatggcg cggctatacc aggccggaac atgccgaggc
      421 ctatgaaggc atgctgaagc ccgagctcct gccgggcgtt ggaaagatcc ccgggtacaa
      481 gggaagctat cttctcagga gagaagtcgg cggcgaggtg gaattcgtca ccgtcatgct
      541 ctgggattcg atcgacgcca tccgcgcggt cgccggggcc gactatgaaa aagcggtggt
      601 tcccgaagag cggcgcccat atctcaagcg gtacgacgcc gcgtccgctc actacgacat
      661 cgtcgcgacg catgcagcgc cgtcaggcga gaaatgacgc gtgcggaaat cctcccccgc
      721 gaagcggggg agggggaccg cccgaagggc ggtggagggg gcctcggcgg tcgggattgt
      781 cctgagagac cgttgccccc tccaccatgc ttcgcatggt tcccctcccc cgcttcgcgg
      841 gggaggataa gcgccggcgg ctttaggcgg cttcggtgaa ttccgggaag acgctgcggt
      901 tccgcccgtc atgcttggct tggtagagag cggcgtcggc ggaggtcagc agagcgtcgg
      961 gagaaatcgt cgcggcgacg atggtcgagc cgccgatgct caccgtgaca tggtcggaga
     1021 cgatcgaggc ttggtgggga atgtcgaggc ggattacttc ccgccgcatc ctttccgcga
     1081 ccgaagccgc gccttgtcgc gtcgtatccg ggaggatcac cgcgaattcc tcgccgccat
     1141 agcgtgcggc ggcgtcgccg gcgcgccaga ggcccttggt caacgcgccg gcgacgaggc
     1201 gcaaacattc gtcgcctttt tgatggccat aggcgtcgtt atattgtttg aagcaatcga
     1261 tatcgagcat cagaagcgag agcggctggc gctcgcgcgc tgctcggcgc gcttccgtcg
     1321 ccagcctttc gtcgaacgat cggcggttca gcagcccggt caggccgtcg cgggtcgcga
     1381 gcgtctccaa ctccatctgc gcctgctttt gcacggtgat gtcgcgcaac gtctcgacca
     1441 ccgcggtcaa gcggccgctg ccgtcataga tcgggccggc gtcgatggcg agatagaggc
     1501 gatggccgat cgtcggcatg acgcaccagt tttccgccga aacgccgtag tcgctgaagc
     1561 cgacattgtt gtagctggcg tagaggtcct tgatgtcctt gtagcgcccg gcgaggacga
     1621 ggtcggcgag acaaggccgc ggctcgccgt aaaaggcgcg ccaatgatcg gactttccaa
     1681 taatttcgtc cgcggaaacg ccggtcaggc gctcgcaggc cttgttccag atcatcacgc
     1741 gacccgaagc gtcgatgacg aaggtcggca ccacgaggtg ctccatgagc cgggtcgcga
     1801 agctccggtc ctcctccgaa ctcaactcca ttgctccccc caacgcgtaa cgaattagac
     1861 tatgacggaa ggattaatga agctttgcgg gcagggccga cgggacggcg gcaaagactt
     1921 tggttggcga atcgtaaaaa aatggcgacg cgcctgctac cgaacctccc tccaattgtt
     1981 cgcgacgaag ccttgcggat cgacggcgac cagccgcttg cccgccaccg cgcggccgcc
     2041 ttggcaggac cacgcataaa tcgtggcgtg gccggtcgcg accatgggga tgttgtcggc
     2101 gtccggattg tcgcggcacc aggcggtcgc gccgggcagg ctgcggcgcg tatcggcctt
     2161 gaagcagtcg agattggcgc cgaccgtgca ggccatcagc ttcgcgccga cgcagcgcac
     2221 gaaagcgttg cgcgccgccg cctcgtcgat gtgcaaggcc ctggcgatcg ccgccgccat
     2281 gcgcggcggc gccgtttcag ggccggcatg cgcgggattg gggcagtagg cctcgccggc
     2341 ggtcgcttgg cgcgccggcg cggcgcctgc aaggacggcc agaaagccgg cgccaaggac
     2401 aagcgatcgc atgtcagcct ccattcgtct gttcagcgcc gtccggaatc gagagcaagg
     2461 cgcgccgcca gggccgccag cacgccgccc atgatccagc gctggacacg cgcccacagc
     2521 ggccggccgg cgaggaaggc ggcgatcgtg ccggcggcaa agacgaagat cgcgttgaag
     2581 gtgacgctga tcgcgatttg cagcgcgccc agcgtcaagg tctggcccaa aatgccgccc
     2641 gaaccgcggt cgacgaattg cggcaacagg gaaagataga gcgcggcgat tttcggattg
     2701 agcaggctgg tcaggagccc catcccgaac agccggcggg gcgaatccgc tttcagctcg
     2761 cgcaattgga acggcgaagc gccgcccggc ttcagcgcgc cccaggcgag ataggcgaga
     2821 taggcggcgc cggcccagcg caatccgtca taggcgtaag gaatcgcaaa gagcagcgcg
     2881 gtgacgccga aggccgcgaa gagaacatag acgataaagc cgagcgcgac gccggcgagc
     2941 gagacgagac ccgcagcgcg gccctgacac accgaacgcg agacgagata ggccatgttc
     3001 ggcccaggcg tcagcaccaa gcccagcgcc acagcgccga aagcggcgag attggcagga
     3061 tgaatcattg agccccaggc gtcgcgtccg atcgcggaag acgtcatcgc gcgtcagccg
     3121 tgctttgtaa atccgtcggc gctccaactt tcgctgccga cgccatgatg aatgtagaag
     3181 aggccgtgag gatcgtattt ctccttgacg gcgcggagcc gcgcgtaatg gccgccccaa
     3241 taggactctt gccaattcgg ctcgaagaaa tcgctctccc atatatagga ggcggcgcgt
     3301 ggaacgagct tgcgcaattc gcccatcgcc tttgtgacgg aagcggcgtc gcttcgcgcc
     3361 gcggctgcat cgggctcgtg tccggcgacg cccggatagg cgggctgctc gtgcgcgccg
     3421 ctgatcgcca gcgcgaaagc gtcgaccacg gccgggttca tcgccgtatc cttcgccgaa
     3481 tcgatcgtct cgggagaagc gccggcgagc cctttgttga agtgaaggga gacgccccaa
     3541 tgttgggccg ccgcaaacag cgcgtcgacg aggctttgcc gccggtcgtc gtcaagcagg
     3601 gaggcgggca gccacagcga ttgataggcg tgtagaactt cccccgcctc gccgagattg
     3661 ccggcccaga aaatattgtc ggccggcgcg ccttggcgat catcggcgat gaccaggccg
     3721 ggaacgctct tcagcgccgc cgggtcccaa aaatgccgcg ccggaacggc gaggacctgc
     3781 ggcccggcct gcatcgtgaa atcctgcggc gcggctttga tccaatcgaa aaagggacgc
     3841 caggcggcct cggcctcgct ctgttcgagg ccctgaaaga ccatcgcaac ggccaggacg
     3901 ccgcccggcc gaaagaccaa ttgctcgccc caatgcgaat tgaacagctt gtcgcgatag
     3961 agcgcgacga ttttatcgat caggcggcga taggcgtcgt ccgagccggc tttgatcgtc
     4021 gcgaaaaccg cgccgaaaat ttgcggcaaa tcgtgcgtgc gcacggtgac gcgggtgacg
     4081 acgccgagcg cgccgccgcc gcctttgagc gcccagaaca ggtcgggatt cgtgcaagaa
     4141 ttggcgagct tgacctcgcc gtctgcggtg acgatctccg cctccagcag gctcgccgcg
     4201 gccgtcccaa aggccttgga gaagctgccg aaaccgcctc ccaggatcaa accggcgacg
     4261 ccgaccgtca tgcagccgcc gccttgaaca tagcggccgc tctttgtcgt cgcttgattg
     4321 taggtatggc cccaaatcgc gccggcgccg atcgttgcgg ccggcgccgg ttcgctccgc
     4381 ccggcgcagc cgacgccgac aaacgcgtcg tgcagcgcga ccgtgttcat cttgcgcgtc
     4441 cagatcaaga gggaatcggc ggcgttcgat tgaccttggt agctgtggcc gccgcccttc
     4501 acgacgagtc gcaaattgta cttccgcgcg aaattgacag cggcgacgat atcgctcgtc
     4561 gtttcggcgg cgaccgcgta gacgcttggc cgcgacgtcc aggcgtcgac ccagccgagg
     4621 gtttgggtca gcgcggcttc gtcgccgaga taataggggt tcttgagttc cttgaagact
     4681 tgcgcgcaag ccgcgccgga cggcgcttcg aagcaagcgt cgagcggcga gcgcaccttg
     4741 atgagccggc cgccgacctc gctgttaagc tgctcccagc tttcgtcgga aggccagccc
     4801 ggatcgccgg ggcggacgcg cgcggtcggg gcggcctgag ccggcgtcag cgccgatcgc
     4861 caaagggcgc ttgcaaaggg aaggccggcg gcgatctgca acaaagctct tctgttcatg
     4921 ctatgaggcc tcgggttaca atgcgctcga aagccggtga gcctattcca gcataaaagc
     4981 tccctcggcg gcggcaaatc cggtcaggcc ggtccatgac atgcggcggc cagactggag
     5041 gcccaataag aatgacaggt cacggaagca gcgaatgacc caaagctcgc aaactctggc
     5101 ccgcgcgccg cttttcgctt cgctcggcga agcggatatc cgcgcgctcg attcccgttg
     5161 tgtttggcgc aaggtcaagg cgggcgcctg ggtgatcgac gatcacgccg acggcgccga
     5221 cgttttcttt gtcgtggatg gtcatgcgcg ggtggtcatc gccgggtcga agcgcgacat
     5281 catcctgcgc gacatccttg acggcgaata tttcggcgag ctctcggcaa tcgacggcaa
     5341 gccgcgttcc gccggcatcg tcgcgatcac cgatacggtt gtcgcgcaaa tgtccgccaa
     5401 gacgttccgc gaaatgatcc atcagcatcc gagcgtctgc gaccaggtgc tggaaaggct
     5461 cgccgcctcg attcgcgcgg tcaacgaccg ggtcaatgag caggtgaatt tcgaggcgcg
     5521 cgatcgtctg atcttggagt tgctgcggct gtcgcgggag agggaaggcg gacgcgtcgt
     5581 cgtgtcgccg ccgccgaccc atgcggaaat cgccgcgcgg atcggcgccc atcgcgaggc
     5641 ggtgaccaaa ttcttgaacg cgctcgagcg cgagggcgcc atctcgcgaa cccgcggcgc
     5701 gatcgtcctc accgatccgg agcttctgcg caacatggtc gaccgcgccg catgagctgg
     5761 accgattgtg atcgccgacc tcgcgtagca aaaaaaagcc ccgcccaggg aggagggggc
     5821 ggggcagttg aggcggggcg ccttaagagc gatcgtcaag tcgggccgtc tcaataagga
     5881 tcgtggatcg ggttgcgaag ccaaatccgc cgcaggccga cagccgccaa ggttccgata
     5941 aggccagcgc ccgctaggat ggcgatctta ccggcctcgg cggcgaaact ttcgtttgtt
     6001 ccttcgtttt gctcgaccgc cgcaaccgac gtcgatccgg cgttcggatt cgacgccgct
     6061 aatgcgcgcc cgtcagaata gccgagagcg acggcgagaa ggccaatgaa gaggaggctg
     6121 cgcatggcgg tcactcctta agttcaaccc gcgcctgatg actgctcggt ccgctcaggc
     6181 ttctgtttcc tttcgtcaaa aagatagggc gagagcggcg acatgggtgt gacgtttctc
     6241 acaaacctgt cttgccggcg gccttcgcag gcggggagcc ggatgtcata gcttgctata
     6301 atgaccgcgc aatgttatag cttgctatga aatagcgcga agaggaatga tcccatggtt
     6361 cgtctcctca agatctgcca tctcctcggc ctgacgctct tcctcggcag cattctcggc
     6421 cacattgtcg ccagcgtggc ggcaggcgcg ccgggcgggg aaagcttcct ctatgcgcgc
     6481 gagcacatcg cgctggcgac gcattttctg acgctgccgg gcttggcttt gtccatcgtt
     6541 tcgggaattg cgctcgtcgc tttgtcgcgc ctttcgccgc tgcgccatcg ttggcttgcg
     6601 gcgaagatcg gcctgaccct cgtcgtgatg gtcaattcga cgctttttct catcccggcc
     6661 ggagcgcggg ccttggcagg cgcggtcgcc ctttcgagag gcgacgccga cgccatggcg
     6721 gagattgtcg cggctttgcg cgtcgaacaa catgccggcg cggcgaatat cctgctgatc
     6781 ctgctgatcg tcgttttggg agtcttcaag ccgcggctac aatcgcgccg gcgagccgcc
     6841 ggcaaggtcc tttcctccag cggcgcggca agccggtgag ctggaaggcg cgatggcgaa
     6901 gaaagcgggg cgcgcggcgc cgtggcggta caggcgcgag gagagcttag gctatctcgt
     6961 caactatctt ggccgcgtgt tggcgcgcgc gctcgagcgc cgcctcgtca agcatggcgt
     7021 gacgctcggg caattcccat tgcttttgac tctttgggag gaggagagcc tcacccaatc
     7081 ggaaatcgcc cggcgcctcg atatcgaaca gccgaccgtg gcgaacacct tgcgccgcat
     7141 ggagcgcgac ggcctggtca aggccgagcc ggacccttcg caccggcggc aagttctcat
     7201 caagctcacc gagaaaggcc gcaagctcga acgccctttg aagctggagg cgctggcggt
     7261 caaccaagcc gccgccgcca aaatctccgc gtccgatctc gaagcgttcc gccgaacgat
     7321 tcgcctgctg ttggaaacct tggaggggcg gacggacgcg taaaaaccgt cattgcgaga
     7381 gcgcagcgac gaagcaattc atgcgtaggg tacggtccta caatggattg cttcgcttcg
     7441 cgagcaatga cgatgcctgc gtctagccgc cggcctcgac cgcggcgatc gcggtgatgt
     7501 tgacgatgcc gcgcgccgtc accgacggcg tcaggatatg cgccggcttg gccgggccga
     7561 tcaggatcgg gccgaccggc agggcgccgg cgatcacccg cgtcaattgc agcgcggtgt
     7621 tgccggtgtc gagattgggg aagatcagca cattggcttc gccgttgagc cgcgacccgg
     7681 tcaggacgcg ctcgcgcgcg acgatagaaa gcgccgtatc ggcctgcatc tccccgtcca
     7741 cttcgaggtc cggcgcgcgc tggcgcaaaa tgtcgagcgc cttgcgcatt ttgagcgccg
     7801 atggcgtatc ggcggagccg aaatccgaat gcgagatcag cgcgattttc ggcgtcacgc
     7861 cgaagcgccg cacatggctt gcgctggcta tcgcgacatc ggcgacctct tcggcggaag
     7921 gatcgtagcg cacatgcgtg tcggcgaaaa agaaggcgcc gctttgcgcg atgacgaggg
     7981 agagcgccgc gcaatcctca aagccgggcg ccaggccgat aatgtccttg atcagcctga
     8041 gccgcgtctg aaagcgccca tcgaggccgc agatcagcgc gtcggcttcg ccgcgccgca
     8101 ccgccagcgc gccgatcacg gtcgtgttgg tgcgaaccac ggtcttggcg gcgctcggcg
     8161 tcacgccctt gcggccggcg acgtcgagat aggaggcgac ataggcgcga tagcgcggat
     8221 catcctcggg atcgacattt tcgaaatgct ggccgggtac gaggccga
//
LOCUS       DATGJW010000062        18819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_840998, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000062 DATGJW010000000
VERSION     DATGJW010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 18819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18819
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_840998"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            471..1199
                     /locus_tag="VKU03_01810"
     CDS             471..1199
                     /locus_tag="VKU03_01810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70022.1"
                     /translation="MIQGTFRLSAIALCATALASLIPLSSAGAASAEMRRHGAAAIHR
                     TAPSTVMRSGHAMTLRSGHRFAARAHFAGRRYGYHRHYRHGYAVVGGVIGGGYAYPYY
                     TGGYYAYGYPRHHSCWWYYRYDPADTPSWCAAYGYAYPSYEYDYAYAYPSYGFAFGFG
                     EGFRRHRHHHFAFADHRFGRHFAFHGAASPVEGRAASVSGGAMRHDFGAAHFGAGAHM
                     TGGTHFMSGGAGAGAHVGGGHYIH"
     gene            complement(1273..2508)
                     /locus_tag="VKU03_01815"
     CDS             complement(1273..2508)
                     /locus_tag="VKU03_01815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70023.1"
                     /translation="MSTVQDTTETAREAPALAAPIRSAETTTVAIILAVSVSHMLNDM
                     MQSLAPALYPVFRNEFRLTFFQTGLITFTFQVTASLLQPLIGLYTDRRPKPFSLPVAM
                     VFTFCGLAVLAYGDAYATLLVAVGLIGIGSAIFHPEASRVARAASGGRHGFAQSLFQV
                     GGNFGQSLGPLMAAFIVVPYGQRSVLWFTALALAAVAILSRVSLWYARRRPTAKHAHA
                     AKVSALPRSVVKRSMAILVVIMFSKVFYLSSLSSYYTFYLISTFRVSVQDSQLYLFIF
                     LAAAAVGTFIGGPVGDRFGRKFVISGSIIGVLPFTLLLPHLNLFWTAVDSVMIGLILS
                     SAFAAIVVYAQELMPGNIGAVAGLFFGLSFGLGGIGAAVLGEIADWTSIGFVYKICAF
                     LPAIGLLTCFLPDVRTRRG"
     gene            complement(2684..3211)
                     /gene="infC"
                     /locus_tag="VKU03_01820"
     CDS             complement(2684..3211)
                     /gene="infC"
                     /locus_tag="VKU03_01820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HLJ70024.1"
                     /translation="MKAVATPEKGGPLTNREIRAREVQLIDADGHNRGAMPIADALQL
                     AEEAGLDLVEIVPNASPPVCKILDFGKFRFLEQKKAAEARKRQKVVEIKEIKLRPGID
                     DHDYEVKMRAVRRFFEEGDKVKVTLRFRGREMAHQDLGFKLLNRVRAETGPFAKVEAE
                     PLMEGRQMTMVLAPK"
     gene            complement(3440..4072)
                     /locus_tag="VKU03_01825"
     CDS             complement(3440..4072)
                     /locus_tag="VKU03_01825"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLJ70025.1"
                     /translation="MSPAKLALLSAIWLAGATLPAEANILIQIDQSTQRMSVDADGQH
                     LYDWRVSTGRPGYDTPNGTFRPNRMDADHFSQEYDNAPMPNAIFFDLQGHAIHGSYDP
                     VGHPAASHGCVRLDPANAAKLFDLVKAEGMANTTVEITGDVRVALRNAKPAPQVRTAR
                     RQPLPSEPDANWDDDAQQLGSYDAYGYPRQPQLDEPSYGYYPAPQPGWKW"
     gene            4557..4859
                     /locus_tag="VKU03_01830"
     CDS             4557..4859
                     /locus_tag="VKU03_01830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HLJ70026.1"
                     /translation="MYLSNQSLIVIVVVGVIAGWIAGQVVAGGGFGFIGDLIVGILGA
                     FIGDWLLPRLGVHFGVGLLALIVNAAIGAIVLLLILRLLTGGWRRNPGWGWRNRRW"
     gene            complement(4993..5988)
                     /locus_tag="VKU03_01835"
     CDS             complement(4993..5988)
                     /locus_tag="VKU03_01835"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008465 - glycerate dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycerate dehydrogenase"
                     /protein_id="HLJ70027.1"
                     /translation="MSKRKLLVVVTRKLPERIETRMRELFDARLNLDDKPMSGAELAA
                     AMREADVLVPTVTDRIDSGLIAQAGEQMKLIANFGNGVDNIDVISATRRGIIVTNTPG
                     VLTEDTADMTMALILSTARRLAEGARIIPNGEWPGWSPNWMLGRRITGKRLGIVGMGR
                     IGQALARRARAFGLSIHYHNRRRVAAEVEEALEATYWESLDQMLARMDIVSVNCPHTP
                     ATYHLLSARRLKYLRPEAIVVNTARGEVIDENALARMLEAGEIAGAGLDVFEREPAVS
                     DKLVKLAAAGKVTLLPHMGSATQEGRNDMGEKVIVNIRTFMDGHRPPDRVLPSML"
     gene            6236..6772
                     /locus_tag="VKU03_01840"
     CDS             6236..6772
                     /locus_tag="VKU03_01840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HLJ70028.1"
                     /translation="MLTLRTSLFRFFHLAAALVATALFLPKLAEAQQVGTVTGRLVPR
                     YVSLKTDRVNLREGPSKDHPTIWVFERAGLPVEITAEFDTWRKIRDSEGAEGWVLHSL
                     LSGRRTALVGPGKQGQIFKLYSRPSDKAALVAKLESGVIGNVRACDGQWCRIEGEGFD
                     GYIQQVELWGVYPNEKVE"
     gene            complement(6847..8085)
                     /locus_tag="VKU03_01845"
     CDS             complement(6847..8085)
                     /locus_tag="VKU03_01845"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HLJ70029.1"
                     /translation="MSQSIGDPESVGMSGRRLERIKPAMQAYIDRGVYAGISTLVARR
                     GKIVHAAEVGWRDKEAKAPMKADTIFRLYSMTKPIVCAALMTLYEEGRVRLIDPVANY
                     IPAFGAVKVTTAYGGLADPARPPTVGDLMSHTSGLTYHFLDDWPVARRYEKAKLLDAG
                     ATLEAAIDELAGLPLAFQPGSRWHYSVGIDVAGRVIEAISGRPLGEFLHERIFAPLGM
                     ADTAFGVPAEKSGRVAAMYGRPDILAPGTTARIAQDAWRRGVNERLDVSATYPVDKPK
                     TFQRGGHGLFGTAGDYLRFAQMLLNGGTLDGARILGRKTLALMHANHLRPDLLPIEIG
                     GLPLLGYGFGLGSRVLMDAAQAGSVGSGGEYGWAGAAKTHYWVDPAEEIVAVFMTQSM
                     LSYDLPELDLRALVYQAIVD"
     gene            8289..9632
                     /locus_tag="VKU03_01850"
     CDS             8289..9632
                     /locus_tag="VKU03_01850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActS/PrrB/RegB family redox-sensitive histidine
                     kinase"
                     /protein_id="HLJ70030.1"
                     /translation="MSALGDDDFGQLARQLRVDTLIRLRWLAVAGQSAAVLLTRFGLG
                     FDVPIGWCFLSIGFAVLLNLGLRLRYPVSRRLADNEATAILSFDIFQLAALLFLTGGL
                     VNPFSILFLAPVMISAASLPLRRTFLLLVLVLGCATVLTFWRRPLPWLPGQDLQLPML
                     YSVAIWAALAVGAAFIAIYATRVAEEARQLAGALTATELALARAQHLTQLDGLAAAAA
                     HELGTPLATLALVVKEMTRLKPPPGPYADDLALLAQEIGRCRAILGKLASLGEDSAGP
                     LGEMTIGQLIEEIVAPHRPLDIAILVETKGEPPEPTCRRNPGMLYGLRNIIENAVDFA
                     EKQVRVAASWDEDQVKIVIADDGPGVAPHILARLGEPYITTRGEDGRDGGGGLGLGLF
                     IAKTLLERSGAATTIVNATPPATGAIATIAWTRGAFEQGRRQPQEVLEGETAAAN"
     gene            9670..10233
                     /locus_tag="VKU03_01855"
     CDS             9670..10233
                     /locus_tag="VKU03_01855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ActR/PrrA/RegA family redox response regulator
                     transcription factor"
                     /protein_id="HLJ70031.1"
                     /translation="MEHQREIEAATAAADKSLLIVDDDKPFAARLARAMEGRGFAPIL
                     AGGVGEGLAAIADKPPAFAVIDLRLGDGSGLDVIRELKAVRPDARAIILTGYGAIATA
                     VTAVKLGAFDYLAKPVDADEILAALMATRADKPQSAEHPMSADRVRWEHIQRVYESCD
                     RNVSETARQLNMHRRTLQRILAKRAPR"
     gene            10539..11813
                     /gene="urtA"
                     /locus_tag="VKU03_01860"
     CDS             10539..11813
                     /gene="urtA"
                     /locus_tag="VKU03_01860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181067.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033219 - urea binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter substrate-binding protein"
                     /protein_id="HLJ70032.1"
                     /translation="MLRATGALIGGAIAFGAFAAQAEDTIKIGILHSLSGTMAISETT
                     LKDVMLMLIAEQNKKGGVLGKKLEPVVVDPASNWPLFAEKARELIAKDKVAVVFGCWT
                     SVSRKSVLPVFQELDSILFYPVQYEGEESQRNVFYTGAAPNQQAIPAVDYLMSSDGGS
                     VKRWVLEGTDYVYPRTTNKILEAYLKAKGVPPEDIMVNYTPFGYSDWQTEVSKIKAFG
                     SAGKKTAVVSTINGDANVPFYKELGNQGVKATDIPVVAFSVGEEELAGLDTKPLVGHL
                     AAWNYFESIDTPENKAFIDQWHDFIKNPKRTTNDPMEAHYIGFNMWVKAVEKAGTTDP
                     DKVIDALIGVSVPNLTGGFSTMMPNHHITKPVFIGEIQADGQFNTVWQTPGTVVAQEW
                     SPYLEGSRDLIADWRKPLSCGNFNVKTGKCGG"
     gene            12021..13610
                     /gene="urtB"
                     /locus_tag="VKU03_01865"
     CDS             12021..13610
                     /gene="urtB"
                     /locus_tag="VKU03_01865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtB"
                     /protein_id="HLJ70033.1"
                     /translation="MLARSLYALLALLSLVAALGAPARADPLDDTLARFAADKYSESE
                     KAVGELAASGAPAAQTIIEALSDNRLYYDAARKVYFKDPSGALLDAKTGAKAIAANLK
                     KVRVNNGLRSAIAAALGAMTLAAPDPDKRIAAAEAVFKSRDVKALASVEAALAKETDP
                     AAAEALRQARAAIRLAGPGASADDRLAAIAVLKTRGDQDAQNMLDLVSQKEPAGPVKA
                     AAQAALSAIQFRLALWSVAQNLWYGLSLSSVLLLAAIGLAITFGVMGVINMAHGEMTM
                     LGAYTVFVVQSLLPPSLSEWSLAISLPLAFAVAACAGVVIERLVIRFLYGRPLETLLA
                     TWGVSLILQQAVRTAFGANNRQVYAPLFMSGGVTLGGLEITFGRLWIIGLAAFVFVAL
                     QLALRFTPFGLRMRAVTQNRRMASAMGISTGLIDMLAFALGSGVAGVAGVALSQIDNV
                     SPNLGQGYIIDSFMVVVLGGVGNLWGTLLGSLTLGVANKLLEPFAGAVLGKIALLVFI
                     ILFIQKRPRGLFALKGRAIEA"
     gene            13607..14746
                     /gene="urtC"
                     /locus_tag="VKU03_01870"
     CDS             13607..14746
                     /gene="urtC"
                     /locus_tag="VKU03_01870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter permease subunit UrtC"
                     /protein_id="HLJ70034.1"
                     /translation="MIAARFPGRLGGTGAAFVLVLGAAAIVVPVLALAIPRASPLYIP
                     PYVVALLGKYLCFAILAVALDLVWGYCGILSLGHGAFFALGGYAMGMYLMRQIGARGV
                     YANPVLPDFMVFLNWHALPIAWRGFDSFPYAMTMVMLVPGILAFAFGWFAFRSRVNGV
                     YLSIITQAMTYALKLAFFRNDFGFGGNNGLTDFKDILGIPLQADATRAGFFSISALFL
                     ILAYLAAKAIVSSKYGKALIAVRDAESRIRFLGYRVENYKLFVFVVSAAMAGIAGALY
                     VPQVGIINPSEFDPANSIEAVIWVAVGGRGTLVGAALGAVLVNFAKTYFTGALPEYWL
                     FALGGLFVLTTLFLPKGILGVLTRRGGRRAGPAFKALAPRQGAAE"
     gene            14743..15504
                     /gene="urtD"
                     /locus_tag="VKU03_01875"
     CDS             14743..15504
                     /gene="urtD"
                     /locus_tag="VKU03_01875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding protein UrtD"
                     /protein_id="HLJ70035.1"
                     /translation="MTADNAKLTQSLLYLDGVTVSFDGYKALRGLSLALEPGEMRAII
                     GPNGAGKTTMMDVITGKTRPDRGDVYFDGRYDLTQLDEAEIAQLGIGRKFQKPTVFES
                     HTVYDNILLAAKAPREVLSTLFGRDGARIGEHIDDILRTIRLADRRDRLAGALSHGQK
                     QWLEIGMLLAQDPKLLLVDEPVAGMTDAETAATADLLRDIARDHSVVVVEHDMAFVRA
                     LGVKVTVLHEGAVLAEGALDQVSADERVIEVYLGR"
     gene            15682..16377
                     /gene="urtE"
                     /locus_tag="VKU03_01880"
     CDS             15682..16377
                     /gene="urtE"
                     /locus_tag="VKU03_01880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033221 - ATPase-coupled urea
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0015840 - urea transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urea ABC transporter ATP-binding subunit UrtE"
                     /protein_id="HLJ70036.1"
                     /translation="MLSVDKIDLFYGAAQALRGVSLKAEIGKVTCVLGRNGVGKSSLL
                     RAIVGLEAIKAGAICWRGEEMSRLPAHRRARRGVGYVPQGREIFPLLTVRENLLTGLA
                     TQPRATRFIDEEIFNLFPVLSDMLGRRGGDLSGGQQQQLAIARALVTRPKLLVLDEPT
                     EGVQPSIIKDIGRAIAYLRRKGDMAILIVEQYFEWARDLCDDYVVMDRGAVALSGERA
                     GMVESEVRRHMSV"
     gene            complement(16523..16972)
                     /locus_tag="VKU03_01885"
     CDS             complement(16523..16972)
                     /locus_tag="VKU03_01885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007599174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ70037.1"
                     /translation="MVNNKEKAHAAGRKPSRPSPADYESLAEFRFQIRRFLEFSEAAA
                     RAVGLTPRQHQALLAIKGLPSGAAMAVGDLAARLGIRHHSAVELADRLAEAGLIARSA
                     DSGDHRRVLLSLTERAESRLAELSLAHLDELARIEPVLKKVLARKAG"
     gene            17064..>18819
                     /locus_tag="VKU03_01890"
     CDS             17064..>18819
                     /locus_tag="VKU03_01890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015599356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HLJ70038.1"
                     /translation="MIVRSASDQPKSPSPIPARSDRRPLGDFTTDPRVLIIAGIAVIV
                     ATGGVAAGVLLLKLIRLATNIAYFGRFDLADLKLQDSPLGPWAVLVPVVGALIVGIMA
                     RFGSEKIRGHGIPEAIEAILLGRSRLDAKVAVLKPLSSAVAIGTGGPFGAEGPIIMTG
                     GAIGSLIAQMLPVSDNERKTLLVAGAAAGMTTVFGTPIAAIMMAVELLLFEWTPRSFI
                     PVAVAAVVAAVERSMLHMPSPLFPFSGGADITLVGLACWLAVGVLAGALSGLLTQLVY
                     ACEDGFQKLPIHWMWWPMLGGVVVGLGGLVEPQALSVGYDNIAGMLQGNVLPKAALLL
                     LVCKAIIWAVALGSGTSGGVLAPLLIMGGALGTVIGGFLPFGGAGFWALLAMAATMGG
                     TMRSPLTATFFAVELTGNVHMLLPLIAACGAAHAMTVLLLRRSILTEKVARRGHHLVR
                     EYRVDPFALTRVRQVMTTAVETVPATTTLHGAAKLLTDPATSHPSFPVVDAEQKVLGV
                     IDPPAVLRWRRSGKHRTTTLAELLAGTKVFSAFPDEYLESLADRMTTANVAHLPVISR
                     EDARLVGYIGWKDLMRVRT"
BASE COUNT         3014 a         6223 c         6163 g         3419 t
ORIGIN      
        1 ttggtcttgc cggccccggc gccggcgacg acgaggagcg gggcggggcc ggccggatcg
       61 ccgagcccat gctcgaccgc gctcgcttgc gcctcgttca aggcgctcga tatcggacgc
      121 tcgcgaatcg ctatggccgc catgcgttcc aggaatcatg tttcgggctc ggctccaagc
      181 cgtctcgtcg caggcgggct tgtaggcgcg gtcgccgacc gcgccgcctc cggcgcgaag
      241 cgcgcgctcg gggcggccgc ggtcagcgac cgcggctacc aaggcctcgg ctgctcgtta
      301 caccggaatg tgaagcattg ttcatgcggg attcagcaag gcagcgtaga gtggccgtta
      361 aagggatgaa aatctggaga aaagagcgct tggcaagcgc tcgtctcgtc ctatctcccg
      421 ggatgaaagg ggcgacctgc ggcgcgctgc gccgcgaata ggaggtcgtc atgatccaag
      481 gaactttccg actttcggcc atcgcgctct gtgcgacggc tttggcgtcg ctgattccgc
      541 tttcgtcggc cggcgcggcg tcggcggaaa tgcgccgcca cggcgccgcc gctatccatc
      601 ggacggcgcc gtcgaccgtc atgcggtccg gtcacgcgat gacgctccgc agcggccatc
      661 gcttcgcggc gcgcgcgcat ttcgccggcc gccgctatgg ctatcatcgc cattatcgcc
      721 acggctatgc ggtcgtcggc ggggtgatcg gcggcggcta cgcctatccg tattataccg
      781 gcggctatta cgcctatggc tacccccgtc atcatagctg ctggtggtac tatcgctatg
      841 atccggccga cacgccgtcc tggtgcgcgg cttatggcta cgcgtatccg tcctacgagt
      901 acgattacgc ctatgcctat ccgtcctatg gattcgcgtt cggctttggc gagggcttcc
      961 ggcgccatcg ccatcaccac ttcgctttcg ccgaccatcg cttcggccgc cacttcgctt
     1021 ttcacggcgc ggcaagcccg gtggaggggc gcgcggcgag cgtgagcggc ggcgcgatgc
     1081 gccatgattt cggcgccgct catttcggcg ctggcgcgca tatgacgggc ggaacgcatt
     1141 tcatgagcgg cggcgccgga gccggcgcgc atgtcggcgg cggacattac atccactaag
     1201 ccgactgcac gacgctcagc ttgcgcaagg cgcgccaatc ccgcgggccg ccgccggccc
     1261 gcgggccagc gcttatcctc gccgcgtcct cacatccggc aggaaacagg tcaaaagccc
     1321 gatcgccggc aggaaggcgc agattttgta gacgaagccg atgctggtcc agtcggcgat
     1381 ttcgccgagc acggcggcgc cgatgccgcc aaggccgaaa gagaggccga agaagagacc
     1441 ggccaccgcg ccgatattgc ccggcatcag ctcctgcgca tagacgacga tcgcggcgaa
     1501 ggccgaggac aggatcaggc cgatcatcac gctgtcgacc gccgtccaga acaggttgag
     1561 atgcggcagg agcagcgtga agggcaaaac gccgatgatc gagccggaaa tgacgaattt
     1621 gcggccgaac cggtcgccga ccgggccgcc gatgaatgtg ccgaccgccg cggcggcgag
     1681 gaagatgaag agatagagct gcgaatcctg caccgacacg cggaacgtgc tgatcagata
     1741 gaatgtgtag tagctcgaca ggctcgaaag gtagaagact ttcgagaaca tgatgacgac
     1801 caggatcgcc atcgagcgct tcaccaccga gcgcggcaag gcggagacct tcgccgcgtg
     1861 tgcgtgcttg gccgtcgggc ggcgcctggc gtaccacagg ctgacgcggc tcagaatggc
     1921 gaccgcggcg agggcgagcg cggtgaacca gaggaccgag cgctgcccgt aaggcacgac
     1981 gatgaaggcc gccatgaggg gcccgagcga ctggccgaaa ttgccgccga cctggaatag
     2041 cgactgggcg aagccgtgcc ggccgcccga ggcggcgcgg gcgacgcgcg acgcttcggg
     2101 atggaagatc gccgagccga tcccgatcag gccgacggcg accagcagcg tcgcataggc
     2161 gtcgccatag gcgagcacgg cgaggccgca aaaggtgaag accatggcga ccggcagcga
     2221 gaagggtttc ggccggcgat cggtatagag gccgatcagc ggctggagca gcgaggcggt
     2281 gacctggaag gtgaaggtga tgagtccggt ctggaagaag gtcaggcgga attcgttgcg
     2341 gaacaccgga tagagcgccg gcgccagcga ttgcatcatg tcgttgagca tgtgcgagac
     2401 gctgaccgcc aggatgatcg cgaccgtcgt cgtctcggcc gagcgaatcg gcgcggccaa
     2461 agccggcgct tcgcgcgccg tttcggtcgt gtcttggaca gtgctcatcg aggcgcccgc
     2521 gccggtctgg atgcgacgct gcagggagcg cccggaatca ttaaggcgct tgaaataatc
     2581 cgcaaaaagc tgttgcgcca ctgccgatag ggcatgacgc ccatgcggcg cagcgccggc
     2641 tttcgccggc gcggtcaata agcggcgcgc cgcgcgccgc cctctatttc ggcgcgagca
     2701 ccatcgtcat ctgccgccct tccatgagcg gctcggcctc gaccttggcg aacggcccgg
     2761 tctcggcgcg gacgcggttg agcagcttga agccgagatc ctggtgcgcc atttcgcggc
     2821 cgcggaaacg caaggtcact ttgaccttgt cgccttcctc aaagaagcgg cgaacggccc
     2881 gcatcttgac ctcgtaatcg tggtcgtcga tgccggggcg caacttgatt tccttgatct
     2941 cgacgacctt ctggcgcttg cgcgcctcgg cggccttctt ttgctcaagg aagcggaatt
     3001 tgccgaaatc gagaatcttg cagaccggcg gactggcgtt gggcacgatc tcgaccaggt
     3061 cgagtccggc ctcctcggcg agttgcagcg catccgctat aggcatggcg ccgcgattgt
     3121 gaccgtcggc gtcgattaat tgaacttccc gcgcccgtat ttcgcggttg gttagcggtc
     3181 cccctttctc tggagtcgct acggccttca tgggacgacg aatggcttat ctcctcttgc
     3241 tgttgacacg aatgaaggat gtttgacgcg tctaacacga caagccccgc ggcgcagggc
     3301 tgcgagggct atcaagcacg atgattcatg gattgtatgg cgaagtcaat cgcgggtcgc
     3361 cgatcctcgc gcggaacgct ggtcatcggg cgatgccggc ccgacgcttt atgccggcgg
     3421 cgccgcgccg acgctttgct caccatttcc agcctggctg aggcgccggg taataaccat
     3481 aggacggctc gtccagctgc ggctgccgcg gatagccgta cgcgtcatac gaaccgagct
     3541 gctgggcgtc atcgtcccag ttggcgtcgg gctcgctcgg caaaggctgt cggcgcgcgg
     3601 ttctcacttg cggcgcgggc ttcgcgttgc gcagcgccac cctcacatcg ccggtgatct
     3661 cgaccgtcgt attggccatg ccctcggcct tgacgaggtc gaacagcttg gcggcgttgg
     3721 cggggtcgag cctgacgcag ccatgcgagg cggccggatg gcccacagga tcatagctgc
     3781 cgtggatggc gtgcccttgc agatcgaaaa agatcgcgtt cggcatgggc gcgttgtcgt
     3841 attcctggga gaaatggtcg gcgtccatcc ggttgggccg gaaagtgccg ttcggcgtgt
     3901 cgtagcccgg ccgtccggtc gagacgcgcc agtcatagag atgttggccg tcggcatcga
     3961 cgctcatccg ctgcgtcgac tggtcgatct ggatgagaat attggcctct gccggcagcg
     4021 tcgcgccggc aagccagatc gcagagagca gggcgagctt tgctggactc atcgaacgac
     4081 ccatagtcac atacgccgcc tcggatcggc cggggctgtt tgacgggtga aacacggcca
     4141 ccgtaaggcg ccgtcacccg ccgcttcgct tatagcatgc cgggagcgcc cggtcagacg
     4201 gatggcggcc gtcaatgaag gcgcatatag cgacgattcg gaattcggcg atgcttttac
     4261 gattatcccc attccgcctc gctgcgtctt cctccttaag gatccgcgcc gcggcccgca
     4321 tcgcgcgccg gtcccgccca aacgagcgcc tcggcgagag ggtgagaaga gggcgacttg
     4381 cgccgatatg gggattgagc aaaggcgttt catcgcctcg ctaagatacg cgagaacgga
     4441 gctccttacg gctaccgcct gctcctccgc ctgatatcgc cgatggtcgc cgatagacga
     4501 cggaacattc gaacatgccc ccgcgttcat cgtcgcgtcg tcggggggcc atgggcatgt
     4561 atctttcaaa ccagagtctc attgtgatcg tcgtcgttgg cgtgatcgcc ggatggatcg
     4621 ccggccaggt cgtcgccggc ggcggtttcg gctttatcgg cgacctcatt gtcggcatcc
     4681 ttggcgcgtt catcggggat tggcttctgc ccaggctggg cgtccatttc ggcgtcggcc
     4741 tccttgccct catcgtcaac gccgcgatcg gcgcgatcgt gcttctgctc atcctgcgcc
     4801 tgctaacggg cgggtggcgg cgcaatcctg gctggggatg gcgaaaccgc cgctggtagt
     4861 acccagtagg cgcgcatccc cgcaggacgc cgggcggcct catcgccgcc ctgcgccgcg
     4921 gagacgatcg gccggccgcg accgcgcaag ggcgcgcttc agtaagctgt cgccgaatta
     4981 aatcggtctc gcttacagca tgctcggcag cacccggtcg ggcggacggt ggccgtccat
     5041 gaaggtgcgg atgttgacga tcaccttctc gcccatgtcg ttgcggccct cctgggtggc
     5101 cgagcccata tgcggcagga gcgtcacttt gccggccgcg gcaagcttga ccagcttgtc
     5161 ggagacggcc ggctcgcgct cgaacacgtc gagtcccgcg ccggcgatct cgccggcctc
     5221 gagcatgcgg gcgagcgcgt tctcgtcgat cacctcgccg cgcgccgtgt tgacgacgat
     5281 cgcctccggc cggagatatt tcaggcggcg ggcggaaagc aggtgatagg tcgccggcgt
     5341 gtgggggcaa ttgaccgaga cgatgtccat ccgggccagc atctggtcga gcgattccca
     5401 ataggtcgcc tccagcgcct cttcgacctc ggccgcgact cgccgacgat tgtgataatg
     5461 gatcgagagc ccgaaggcgc gggcgcggcg cgccagagcc tggccgatcc ggcccatgcc
     5521 gacaatgccg agccgcttgc cggtgatccg ccggccgagc atccaattcg gcgaccagcc
     5581 cggccattcg ccgttcggga tgatcctcgc gccctcggcg aggcggcgcg ccgtcgagag
     5641 gatcagcgcc atcgtcatgt cggctgtgtc ctcggtgagc acgcccggcg tgttggtgac
     5701 gatgatgccg cgccgcgtcg ccgagatcac gtcgatattg tcgacgccgt tgccgaaatt
     5761 ggcgatcagc ttcatctgct cgccggcttg cgcgatcagg ccggaatcga tccggtcggt
     5821 gacggtcggc accagcacgt cggcttcgcg catcgccgcg gcgagttcgg cgccgctcat
     5881 cggcttgtcg tcgagattga gcctggcgtc gaacaattcg cgcatacgcg tctctatccg
     5941 ctccggcaat ttccgcgtga cgacgacgag gagtttcctc ttgctcatgc ggcgcggcct
     6001 ttgagagtcg cttgagcctt cacaggccgc attctcggcc tgttgcaaat tcgaaacggg
     6061 gatggattcg tcgcggctct tccaatcgcc tttacgtccc gttaagcgtc aaagcgccag
     6121 atggaggcag cccgcctgtc gccgatctga agcctttctc ggcctctcta gcagaagacg
     6181 gagcaaagac aaggacccgt cgatccatcg gcgaaggagg ccgaaacgcc gagccatgct
     6241 gaccttgcgc acgtcccttt ttcgattttt tcatcttgcc gccgctctcg tcgccacggc
     6301 tctctttctt cccaaattgg ctgaagccca gcaggtcgga accgtgaccg gacggctggt
     6361 gccgcgctat gtcagcctca agaccgaccg cgtcaatctg cgcgaggggc cgtcaaagga
     6421 ccatccgacg atctgggtct tcgagcgcgc cggcctgccc gtcgagatca ccgccgaatt
     6481 cgacacctgg cgaaaaatca gggattcgga aggcgccgag ggctgggtcc tccattcgct
     6541 cctctccggc cgccgcaccg ccttggtcgg tcccggcaag cagggtcaga tcttcaagct
     6601 ctattcccgg ccgagcgaca aggccgctct tgtggcgaag ctggaatccg gcgtgatcgg
     6661 caacgttcgc gcctgcgacg gccagtggtg ccggatcgaa ggcgaaggct ttgacggata
     6721 tatccagcaa gtcgagcttt ggggcgtcta tccgaacgag aaagtggagt agggcgcgag
     6781 ctcccgttgt agccgcggcc ggcgaccgtg cccacagccg gcgacggccg gggcgcgatt
     6841 cgaacttcaa tccacgatcg cctgatagac cagcgcccgc agatcgagct cggggagatc
     6901 gtagctcagc atcgattggg tcatgaacac ggcgacgatc tcttccgcgg ggtcgaccca
     6961 ataatgggtc ttggcggccc cggcccagcc atattcgccg cccgagccga ccgagccggc
     7021 ctgcgccgca tccatcagca cgcgcgagcc gagcccgaat ccatagccga gcagaggcaa
     7081 gccgccgatc tcgatcggca gcaggtcagg ccgcaggtga ttggcgtgca tcaaagccag
     7141 cgtcttgcgg ccgaggatgc gcgcgccgtc cagcgttccg ccattgagca gcatctgggc
     7201 gaagcgcaga tagtcgccgg ccgtgccgaa caggccatgg ccgccgcgct gaaaggtttt
     7261 gggcttgtcg accggatagg tcgccgaaac gtcgaggcgc tcgttgacgc cgcgccgcca
     7321 ggcgtcctgc gcgatgcgcg cggtcgtgcc tggcgccagg atgtccggcc ggccatacat
     7381 cgccgcgaca cggccgcttt tctcggcggg aacgccgaaa gcggtgtcgg ccatgccgag
     7441 cggggcgaaa atgcgctcgt ggagaaactc gccgagcgga cggcctgaaa tcgcctcgat
     7501 gacgcggccg gcgacatcga tgccgacgct gtaatgccag cgcgagccgg gctgaaaggc
     7561 gagcggcagg ccggcaagct cgtcgatcgc cgcttcgagc gtcgccccgg cgtcgagcag
     7621 cttggctttt tcgtagcgcc gcgcgaccgg ccaatcgtcg aggaaatgat aggtcaggcc
     7681 gctcgtatgg ctcatcaaat cgccgaccgt cggcggccgc gccggatcgg cgaggccgcc
     7741 ataggcggtc gtcactttca cagcgccgaa ggcaggaatg tagttggcga ccggatcgat
     7801 cagccggacg cgcccttcct cgtaaagcgt catcagcgcc gcgcagacga tcggcttggt
     7861 catcgagtag aggcggaaaa tcgtgtcggc cttcatcggc gccttggctt ccttatcgcg
     7921 ccagccgacc tcggccgcat gcacgatctt gccgcgccgc gcgaccagcg tgctgatccc
     7981 ggcatagacg ccgcggtcga tataggcctg catcgccggc ttgatgcgct cgaggcgccg
     8041 cccgctcatg ccgaccgatt ctggatcccc gatggactgg ctcacttttt ctcccctgct
     8101 cgccgcgagt cgttgcgcgg cgccattctg cgccagcctt ccgcgaattg tcttcaaaga
     8161 tttgcgagcc gcgccgaagc gttgtcattc cctgggaaag cgggaatcca ggctgagcca
     8221 gttccgcagg cgcggcggtt ctctggattc ctgctttcgc gggaacgaca gagaggtaaa
     8281 atcggctcat gtccgcgctt ggcgatgacg atttcggcca gcttgcgcgt cagctccgcg
     8341 tcgacacgct gatccggctg cgctggctgg cggtcgccgg ccagtcggcg gcggtgctgc
     8401 tgacccgctt cggccttggg ttcgacgtcc cgatcggctg gtgctttctg tcgatcggct
     8461 tcgcggtcct gctcaatctc ggcttgcgcc tgcgctatcc ggtcagccgg cgcctcgccg
     8521 acaacgaggc gaccgccatc ctgagcttcg atattttcca gctcgcggcg ctgctcttcc
     8581 tcacgggcgg cctcgtcaat ccgttctcga tcctattcct tgcgccggtg atgatttcgg
     8641 cggcgtcgct gccgctgcgc cgcactttcc ttctgctggt tctcgtgctc ggctgcgcga
     8701 ccgtcctgac cttctggcgg cgtcctctac cctggctgcc gggacaggat ctgcaattgc
     8761 cgatgctcta ttcggtggcg atctgggcgg ccctggcggt cggcgccgcg ttcatcgcga
     8821 tctatgcgac gcgcgtcgcc gaggaggccc ggcaattggc tggcgccttg acggcgaccg
     8881 agcttgctct ggcgcgcgcc cagcacctga cccaactcga cggcctcgcc gcggccgccg
     8941 cgcacgaatt gggcacgccg ctcgcgacgc tcgccctcgt cgtcaaggag atgactcggc
     9001 tgaagccgcc gcccgggcct tacgccgacg acctggcgct gctcgcccag gagatcggcc
     9061 gctgccgagc catcctcggc aagctcgcct cgctcggcga ggacagcgcc ggcccgctcg
     9121 gcgagatgac gatcggccag ctcatcgagg agatcgtcgc cccgcaccgc ccgctcgaca
     9181 tcgccatcct ggtcgaaacg aagggcgagc cgcccgagcc gacctgccgg cgcaatcccg
     9241 gcatgctcta cggcttgcgc aacatcatcg agaacgccgt cgatttcgcc gagaagcagg
     9301 tccgcgtcgc cgcctcctgg gacgaggacc aggtgaagat cgtcatcgcc gacgacgggc
     9361 cgggcgtcgc gccgcatatt ctggcgcggc tcggcgagcc ctatatcacg accaggggcg
     9421 aggacggccg cgacggcggc ggcggcctgg gcctcggcct gttcatcgcc aagacgctgc
     9481 tggagcgctc cggcgccgcg accacaatcg tcaacgcgac gccgccggcg accggcgcga
     9541 tcgcgacgat cgcctggacg cgcggcgcct tcgagcaagg tcgacggcag ccgcaggaag
     9601 tcctagaagg agaaaccgcc gcggccaatt gacgccgcga cgccaaggcg cagcgaattg
     9661 gatttggcga tggagcacca gcgtgaaatc gaggccgcga cggccgcagc ggataaatcc
     9721 ctcctgatcg tcgacgacga caagccgttc gcggcgcggc tcgcccgcgc gatggaggga
     9781 cgcggctttg cgccaatcct ggcgggcggc gtcggcgagg gcctcgccgc gatcgccgac
     9841 aagccgccgg ctttcgccgt gatcgacctg cgcctcggcg acggcagcgg cctcgacgtg
     9901 atccgcgagc tgaaagcggt caggcccgac gcgcgcgcga tcatcctgac cggctatggc
     9961 gcgatcgcca ccgcagtgac cgcggtcaag ctgggcgcct tcgattatct cgccaagccg
    10021 gtcgacgccg acgaaatcct cgccgctttg atggcgacgc gggccgacaa gccgcaaagc
    10081 gccgagcatc caatgtcggc cgaccgggtg cgctgggagc atatccagcg cgtctatgaa
    10141 tcctgcgacc gcaacgtctc cgagacggcg cgccagctca acatgcaccg ccgcacgctg
    10201 cagcgaatct tggcgaagcg cgcgccgcgc tgaaggcgcc aacccgtcat tgcaggagcg
    10261 cggcgacgaa gcaatccatg catagggcga agtcctacga atggattgct ttcgcttcgc
    10321 tcgcaatggc gggtgcggcc ttttcctcct ccgcctgcat attcccgcgc cacgccgcct
    10381 aatttttgcg cgctcccgcg tcgcccgggc ggcgtttcat gcgtcccggc ctggcacgtc
    10441 ccttgcgagg gcttcccatc ccataggggc ggcggcctca agccgccgcg gaggcgtttc
    10501 gcaagacggg gtttgccatg tcattttctc gacggtacat gctgcgtgcg accggcgcgc
    10561 tcatcggcgg cgcgatcgct ttcggagctt tcgccgcgca ggcggaagat acgatcaaga
    10621 tcggcattct tcactctctg tccggcacga tggcgatcag cgagacgacg ctgaaggacg
    10681 tcatgctgat gctgatagcc gagcagaaca agaagggcgg cgtgctcggc aagaagctcg
    10741 agccggtggt cgtcgacccc gcctcgaact ggccgctctt cgccgaaaag gcgcgcgagc
    10801 taatcgccaa ggataaagtc gcggtggtgt ttggctgttg gacgtccgtc tcgcgcaaat
    10861 ccgtcctgcc ggtcttccag gagctcgact cgatcctctt ctatccggtt cagtacgagg
    10921 gcgaggaatc gcagcgcaac gtcttctata ccggcgcagc gcccaatcag caggcgattc
    10981 cagcggtcga ctatctgatg agctcggacg gcggctcggt gaagcgctgg gtgctcgaag
    11041 ggacggacta tgtctatccg cgcacgacca acaagattct cgaggcgtat ctgaaggcca
    11101 agggcgtgcc gcccgaggac attatggtca attacacacc gttcggctat tcggactggc
    11161 agaccgaggt ctcgaaaatc aaggctttcg gctcggccgg caagaagacg gccgtggtct
    11221 cgacgatcaa cggcgacgcc aacgttcctt tttataagga gctcggcaac cagggcgtca
    11281 aggcgaccga cattccggtc gtcgcctttt cggtcggcga ggaagagctc gccggcctcg
    11341 acaccaagcc gctggtcggc catctcgccg cttggaacta tttcgaatcg atcgacacgc
    11401 cggagaacaa ggccttcatc gaccagtggc atgacttcat taagaatccg aagcgcacca
    11461 ccaatgaccc gatggaggcc cattacatcg gcttcaacat gtgggtgaag gcggtggaga
    11521 aggccggcac gaccgatccc gacaaggtga tcgacgcgct gatcggcgtc tccgtgccga
    11581 acctcaccgg cggattttcg acgatgatgc cgaaccatca catcaccaag ccggtcttca
    11641 tcggcgaaat tcaggccgac ggccaattca acacggtctg gcaaacgccg ggcacggtcg
    11701 tcgcgcagga atggtcgccg tacctggaag gctcgcgcga cctcatcgcg gattggcgca
    11761 agccgctctc ctgcggcaac ttcaacgtga agaccggcaa gtgcggcggt tgattaccta
    11821 ataggaagag gagctgaggc tccccctcgc cccgaccctc tcccgcagcg cccgcagtgg
    11881 gagagggggc gcgccgagcc cgtcatcggt cgtgtcgcgt agccgtcagg cgcggccgcg
    11941 ccccttctcc cgcttgcggg agaaggtggc cgcgaagcgg tcggatgagg gtgagccgcg
    12001 gcgataggaa catagcttgc atgcttgccc ggtcccttta cgcgcttctc gctctgctca
    12061 gcttagtcgc cgcgctcggc gcgccggctc gcgccgatcc gctcgacgat acgctcgccc
    12121 gcttcgccgc cgacaaatat tcggaaagcg agaaggcggt cggcgagctt gccgcgagcg
    12181 gcgcgccggc ggcgcagacg atcatcgagg cgctgtcgga caatcgcctc tactatgacg
    12241 ccgcgcgaaa ggtttacttc aaggatccga gcggcgcttt gctcgacgcc aagaccggcg
    12301 cgaaggcgat cgccgccaat ttaaagaaag tgcgcgtcaa caacggcctt cgcagcgcga
    12361 tcgcggcggc gctgggcgcg atgacgctcg ccgcgcccga tcctgacaag cgtatcgcgg
    12421 ccgcggaggc ggtgttcaaa tcgcgcgacg tcaaggcgct ggcctctgtc gaggccgcgc
    12481 tcgccaagga gaccgatccc gccgccgccg aggccttgcg tcaggcccgc gccgccatcc
    12541 ggctcgccgg gccgggcgcg agcgcggacg accggctcgc cgcgatcgcc gtcctcaaga
    12601 cgcgcggcga tcaggatgcg cagaacatgc tcgatctggt ctcgcaaaag gagccggccg
    12661 gcccggtcaa ggcggcggcg caggcggcgc tttcggcgat ccagttccgc ttggccttgt
    12721 ggagcgtcgc gcaaaatctc tggtacggac tgtcgctctc atccgttctg ttgctcgcgg
    12781 cgatcggcct cgccatcacg ttcggcgtga tgggcgtcat caatatggcg catggcgaga
    12841 tgacgatgct cggggcctat acggtttttg ttgtccagag cttactgccg ccatcgctgt
    12901 ccgaatggtc gctggcgatt tccctgccgc tcgcctttgc cgtcgcggcc tgcgccggcg
    12961 tcgtcatcga gcgcctcgtg atccgcttcc tctatggccg gccgctcgag acgctgctcg
    13021 cgacctgggg cgtctcgctg attcttcagc aggcggtgcg caccgcgttc ggcgccaaca
    13081 atcgccaggt ctatgcgccg cttttcatgt ccggcggcgt cacgctcggc ggcctcgaaa
    13141 tcaccttcgg gcgcctctgg atcattggct tggcggcttt cgtcttcgtc gcgctgcaac
    13201 tggcgctgcg ttttaccccg ttcggcttgc gcatgcgcgc cgtcactcag aaccgccgca
    13261 tggcctcggc catgggcatt tcgacgggcc tgatcgacat gctcgctttc gcgctgggct
    13321 ctggcgtcgc cggcgtcgct ggcgtcgcgc tgtcgcagat cgacaatgtc tcgcccaatc
    13381 tcggccaagg ctacatcatc gacagcttta tggtcgtggt tctgggcggc gtcggcaatc
    13441 tctggggaac gctgctcggc tcgctgacgc tcggcgtcgc caataagctg ctcgagcctt
    13501 tcgccggcgc ggtactcggc aagatcgcgc tgctggtctt tatcatcctg ttcatccaga
    13561 agcgcccgcg cggcttgttc gcgctcaagg gaagggcgat cgaggcatga tcgcggcgcg
    13621 ctttcccggt aggctcggcg gaacgggcgc ggccttcgtt ctcgtcctcg gcgccgcggc
    13681 gatcgtcgtg ccggtcctgg cgctcgccat tccgcgcgca tcgccccttt acattccgcc
    13741 ttatgtcgtt gcgctgctcg gcaaatacct atgcttcgcg atcctcgccg tcgcgctcga
    13801 cctggtctgg ggctattgcg gcatcctctc gcttggccac ggcgccttct tcgccttggg
    13861 cggctacgcg atgggcatgt atctgatgcg ccagatcggc gcgcgcggcg tctacgccaa
    13921 tccggttctg ccggatttca tggtgttcct gaattggcat gcgctgccca tcgcctggcg
    13981 cggcttcgat tcctttccct acgccatgac gatggtgatg ctggtcccgg gaatcctcgc
    14041 cttcgcattc ggatggttcg ccttccgctc gcgcgtcaat ggcgtttacc tttcgatcat
    14101 cacccaggcg atgacctatg cgctgaagct cgccttcttc cgcaacgatt tcggcttcgg
    14161 cggcaataac gggctcaccg atttcaagga tattctcgga attccgctgc aagccgatgc
    14221 gacccgcgcc gggttcttct cgatatctgc cctctttctc atcctggcct atctcgccgc
    14281 caaagcgatc gtctcctcga aatacggcaa ggcgctgatc gcggtgcgcg acgccgagag
    14341 tcgcatccgc ttccttggct atcgggtcga gaactataag ctcttcgtct tcgtcgtctc
    14401 ggcggcgatg gccggaatcg caggggcgct ttacgtgcca caggtcggta tcatcaatcc
    14461 gtcggagttc gatcccgcca attcgatcga ggccgtgatc tgggtcgcgg tgggcggccg
    14521 cggcacgctg gtcggcgccg cgctcggcgc cgtcctcgtc aatttcgcca agacctattt
    14581 taccggcgcg ctgcccgaat attggctgtt cgcgctcggc ggactcttcg tcttgacgac
    14641 cttgttcctg ccgaaaggca ttctcggcgt cctgacccgg cggggcggcc ggcgggcggg
    14701 gcccgccttc aaagctcttg cgccgcggca aggagcggcc gaatgaccgc agacaacgct
    14761 aagctgacgc agagcctgct ctatctcgac ggcgtcaccg tctccttcga cggctataaa
    14821 gccctgcgcg gcctatcgct ggcgctcgaa cccggcgaga tgcgggcgat catcggtccg
    14881 aacggcgcag gcaagacgac gatgatggac gtcatcaccg gcaagacccg gcccgatcga
    14941 ggcgatgtct attttgacgg ccgatacgat ctcacccaac tcgacgaggc cgagatcgcc
    15001 cagctcggaa tcggccgcaa gttccagaag ccgaccgtct tcgagagcca cacagtttac
    15061 gacaacatcc tgctcgccgc aaaggcgccg cgcgaggtcc tgtcgaccct gttcgggcgc
    15121 gacggcgcgc gcatcggcga gcatatcgac gacatcttgc ggacgatccg cctcgctgat
    15181 cgtcgcgacc gtctggccgg cgccctatcg catggccaga agcaatggct cgaaatcggc
    15241 atgctgctgg cgcaagatcc aaagctcctg ctcgtcgacg agccggtcgc cggcatgacc
    15301 gacgccgaga cggcggcgac cgccgatctc ctgcgcgaca tcgcccgcga ccattcggtc
    15361 gtcgtggtcg agcacgatat ggctttcgtc cgcgcgctcg gcgtcaaggt gacggtgctg
    15421 catgagggcg ccgttctcgc cgagggcgcg ctcgaccagg ttagcgccga cgagcgggtg
    15481 attgaagtct atctggggcg gtgaggatga acctccgccg tcattgccag cgaagcgaag
    15541 caatccagga agcgcaggcg tttcctcgac gaagcgcggg gccgaagccc cctctccccg
    15601 caagcgggga gagggggccc cggcgccgcg cctgctccgt caagcgccag cgcttccgca
    15661 ttgctcgcca tcggggcgcc catgctgagc gtcgataaaa tcgatctctt ctacggcgcc
    15721 gcgcaagcct tgcgcggcgt atcgctcaag gccgagattg gcaaggtcac ctgcgtgctt
    15781 ggccgcaatg gcgtcggcaa atcctcgctg ttgcgcgcga tcgtcggtct ggaagcgatc
    15841 aaggccggcg cgatctgttg gcgtggcgag gagatgagcc gccttcccgc tcatcggcgc
    15901 gcccggcgtg gcgtcggcta cgtgcctcag gggcgcgaga tttttccgct gctcacagta
    15961 cgtgaaaatc tcttgaccgg ccttgcgacg cagccccggg cgacgcgctt catcgacgag
    16021 gagattttca atctgtttcc ggtcctttcc gatatgctcg gccggcgcgg cggcgatctt
    16081 tccggcggcc agcagcagca attggcgatc gcccgcgcgc tggtcaccag gcccaagctc
    16141 cttgtcctcg atgagccgac cgaaggcgtc cagccgtcga tcatcaagga tatcggccgc
    16201 gcgatcgcgt atctccggcg aaagggcgat atggcgatcc tgatcgtcga gcaatatttc
    16261 gaatgggcgc gcgacctctg cgacgattat gtcgtgatgg atcgcggcgc ggtggcgctg
    16321 tcaggcgagc gggccgggat ggtcgagagc gaggtgcgcc ggcatatgtc ggtttaggag
    16381 cttcagacga gcggctccgc gcttgttgcg cgggggcggt tctctcgtgg atattcctgt
    16441 atatacgtga ggcggcccct atgaagatcg cccgaacccg gtggtgatcg gccgcgcctc
    16501 cctgcgccgg aggcgcgccg cttcagcccg ccttccgcgc caggaccttc ttcaacaccg
    16561 gctcgatgcg cgcgagctcg tcgagatggg cgagggagag ctcggcgagg cgcgattcgg
    16621 cgcgctcggt caggctcagg aggacgcgcc gatgatcgcc ggaatcggcg ctgcgcgcga
    16681 tcaggcccgc ttcggcgagg cggtcggcga gctcgaccgc gctgtgatgg cggattccga
    16741 gccgcgccgc cagatcgccg accgccatcg ccgcgccgga cggaaggccc ttgatcgcca
    16801 gcagggcctg gtgttggcgg ggcgtcagcc cgaccgcgcg cgccgcggct tcgctgaact
    16861 ccaggaagcg gcggatctga aaacggaatt cggcaaggga ttcgtagtcc gccgggctcg
    16921 gacggctggg ctttcgtccg gccgcatggg ccttttcttt attgttaacc aaagcctcgc
    16981 tccagcttgc ctttttatat cgcaatgcga tacgaggcgc attcctcccc caatatgctt
    17041 attcgcagag attgcttccg tcgatgattg tccgctccgc ttccgaccag ccgaaatccc
    17101 catcgccgat tccggcgcgg agcgatcgcc gcccgctcgg cgacttcacc accgatccgc
    17161 gcgtcctgat catcgccggc atcgcggtga tcgtggcgac ggggggcgtc gccgccggcg
    17221 tcttgctgct caagctgatc aggctggcga ccaacatcgc ctatttcggc cgcttcgatc
    17281 tcgccgatct caagctgcag gattcgccgc tcggcccgtg ggccgtgctg gtcccggtgg
    17341 tcggcgcgct gatcgtcggg atcatggcgc ggttcggcag cgagaaaatc cgcggccacg
    17401 gcatccccga ggcgatcgag gcgatcctgc tcggccgctc gcggctcgac gcgaaagtcg
    17461 ccgtcctcaa gccgctgtcg tccgccgtcg ccatcggcac cggcggcccg ttcggggcgg
    17521 aaggcccgat catcatgacc ggcggcgcga tcggctcgct gatcgcccag atgctgccgg
    17581 tcagcgacaa cgagcgcaag acgctgctcg tcgccggcgc cgcggccggc atgaccacgg
    17641 tgttcgggac gccgatcgcc gcgatcatga tggcggtgga gctgctcctc ttcgaatgga
    17701 cgccgcgcag cttcatcccg gtcgctgtgg cggctgtcgt cgcggcggtc gagcgcagca
    17761 tgcttcacat gccctcgcca ttgtttccgt tcagcggcgg cgccgatatc acgcttgtcg
    17821 gcctcgcctg ctggctggcg gtcggcgtcc tcgccggcgc tctctccggc ctgctgaccc
    17881 aactcgtcta tgcctgcgag gacggattcc agaagctgcc gatccattgg atgtggtggc
    17941 cgatgctggg cggggtcgtc gtcggccttg gcggccttgt cgagccgcaa gcgctgagcg
    18001 tcggctacga caacatcgcc ggcatgctgc agggaaacgt ccttcccaag gcggccttgc
    18061 tgctcctcgt ctgcaaggcg atcatctggg cggtggcgct cggctcgggc acgtcgggcg
    18121 gcgtgctggc gccgctgctg atcatgggcg gcgcgctggg cacggtgatc ggcggcttcc
    18181 tgccgttcgg cggcgccggc ttctgggcgc tgctcgccat ggccgcgacg atgggcggga
    18241 cgatgcgctc gccgctgacc gcgaccttct tcgccgtcga gctgaccggc aatgtccata
    18301 tgctgctgcc gctgatcgcc gcttgcggcg ccgcgcacgc catgaccgta ttgctgctgc
    18361 gccggtcgat cctcaccgag aaagtcgcgc ggcgcggcca tcacttggtc cgcgaatatc
    18421 gcgtcgatcc gttcgcgctg acgcgggtgc gccaggtcat gaccaccgcg gtcgaaacgg
    18481 ttccggcgac gacgacgttg cacggcgccg ccaagctgct caccgatccg gcgacgtcgc
    18541 atccgagctt tccagtcgtc gacgccgagc agaaggtgct cggcgtgatc gatccgccgg
    18601 ccgtgttgcg ctggcggcgc tcgggcaagc accgtacgac gacgcttgcc gaattgctgg
    18661 ccgggaccaa ggtcttctcc gcctttcccg acgaatattt ggagagcctc gccgatcgca
    18721 tgaccaccgc caatgtcgcc catctcccgg tgatctcgcg cgaggacgcg cgcctcgtcg
    18781 gctatatcgg ctggaaggac ctgatgcggg tgcgcacga
//
LOCUS       DATGJW010000063         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_425446, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000063 DATGJW010000000
VERSION     DATGJW010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_425446"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1220)
                     /locus_tag="VKU03_01895"
     CDS             complement(<1..1220)
                     /locus_tag="VKU03_01895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase"
                     /protein_id="HLJ70039.1"
                     /translation="MKAKFYVPGDSAALSVGADGVAQAIAGEAARRGLDVSIVRNGSR
                     GLFWLEPLVEIETSTGRLAYGPIEPEDVASLFDAHFLSGRAHPKALGRIEDHPYLKNQ
                     QRLLFARCGVTDPLSIEDYRSHGGLKGLERALAIGLQAIGEDVLASGLRGRGGAGFPA
                     GIKWRTVAATAADQKYVICNADEGDSGTFADRMIMEGDPFTLIEGMAIAAVAVGATKG
                     FVYIRSEYPHAFAKFNEALAAARKAGLLGRSVLGSPHAFDIETRLGAGAYICGEETSL
                     LESLEGKRGEVRAKPPVPAISGLFGKPTAMNNVLTFASAPWILTHGGKAYADYGMGRS
                     RGTQPFQLAGNIKQGGLVELAFGATIRTLVEDFGGGTRSGRPIRAVQVGGPLGAYFPV
                     SLLETPLDYEAMLEA"
     gene            complement(1217..1696)
                     /locus_tag="VKU03_01900"
     CDS             complement(1217..1696)
                     /locus_tag="VKU03_01900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HLJ70040.1"
                     /translation="MTRKQAWDEALAGRLIEERKGADGALLPILHALQDAFGYIDRAA
                     IAPIAAALNVSKADVHGVVSFYHDFRATPADGHVLKLCRAESCQAMGCEDLVDHLREA
                     HGLTPDRDNGDGLHVETVYCLGNCALSPAAVLDGAPIGRLDAAKLDRVVRQAREAAP"
     gene            complement(1818..2744)
                     /locus_tag="VKU03_01905"
     CDS             complement(1818..2744)
                     /locus_tag="VKU03_01905"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLJ70041.1"
                     /translation="MMLRQLEYLVALAREKHFGRAAAACHVSQPTLSAAIRQLEEELG
                     APIVERGHKFHGLTAQGKAALDHAHRILAEAGALKRDLEEMDRGLSGRLRIGAIPTAL
                     PIIAHITAPFYQRFPNVLVTILSLNSQQIQQGIDDFELDVGLTYLDNEPLSNVRAKPV
                     YVEQYMFLTPSSGPYARRRSITWSEAGGTPLCLLTPDMQNRRIVDGIFRSADASPKPA
                     METNSIFNLCSHVSAGLWSSIVPVQLLQFFGVPRGAKAIELVEPKAQRTIGLIIADRE
                     PPSPLARNLFAMERPADIGSLIVPPVSAAEPV"
BASE COUNT          518 a          948 c         1000 g          473 t
ORIGIN      
        1 gcttcgagca tcgcctcgta gtcgagcggc gtctcgagca aggaaaccgg aaaatacgcg
       61 ccgagcgggc cgccgacctg cacggcgcgg atcggccggc cggagcgcgt gccgccgccg
      121 aaatcctcga cgagagtgcg gatggtcgcg ccgaaagcga gctcgacgag gccgccctgc
      181 ttgatattgc cggcgagctg gaagggctgc gtcccccgcg agcggcccat gccgtaatcg
      241 gcgtaggcct tgccgccatg ggtcaggatc cagggcgccg aggcgaaggt cagcacattg
      301 ttcatggcgg tcggcttgcc gaacaggccg gaaatcgccg gcaccggcgg cttggcgcgc
      361 acttcgccgc gcttgccttc caggctttcg agcagcgagg tctcctcccc gcaaatatac
      421 gcgccggcgc cgagccgcgt ctcgatgtcg aacgcgtgcg gcgagccgag cacggagcgg
      481 ccgagcagtc cggccttgcg cgccgcggcg agcgcctcgt tgaacttcgc aaaagcgtgc
      541 ggatattccg agcggatata gacgaagcct ttcgtcgccc cgaccgcgac cgcggcgatc
      601 gccatgccct cgatcagcgt gaacggatcg ccctccatga tcatgcggtc ggcgaacgtg
      661 ccgctgtcgc cttcgtcggc gttgcagatg acgtattttt ggtcggccgc ggtcgcggcg
      721 acggtgcgcc atttgattcc ggcgggaaat ccagcgccgc cgcgcccgcg caggccggaa
      781 gcgagcacgt cttcgccaat cgcttgcagg ccgatcgcca gggcgcgctc tagacccttc
      841 aggccgccat ggctccgata gtcctcgatc gataacggat cggtgacgcc gcaccgggcg
      901 aaaagcaggc gctgctggtt tttcaaatag ggatggtcct cgatccggcc gagcgccttc
      961 ggatgggcgc ggccggaaag gaaatgcgcg tcgaacaggg aagcgacgtc ctccggctcg
     1021 atcggcccgt aggcgaggcg tccggtcgag gtctcgatct cgaccagcgg ctccagccag
     1081 aagaggccgc gcgagccgtt gcggacgatg cttacgtcga ggccgcgccg ggcggcctcc
     1141 ccggcgatcg cttgggcgac gccatcggcg ccgaccgaca gggccgcgga atcgccggga
     1201 acgtagaatt tggccttcac ggcgccgcct cgcgcgcttg tcgaacgact ctgtcgagct
     1261 tggccgcatc caggcggccg atcggcgcgc cgtcgagcac agcggcgggc gaaagcgcgc
     1321 aattgccgag gcaatagacc gtctcgacat ggagaccgtc gccattgtcg cggtccggcg
     1381 tcagcccatg cgcctcgcgc agatggtcga ccaaatcttc gcaacccatc gcctggcagg
     1441 attcggcccg gcacagcttg agcacatggc cgtccgccgg cgtcgcccgg aaatcatggt
     1501 aaaagctgac gacgccgtga acgtcggcct tggagacgtt aagcgctgcg gcgatcggcg
     1561 cgatggcggc gcggtcgata tagccgaacg cgtcctggag cgcgtgcaga atgggcagca
     1621 gggcgccgtc ggcgcctttc cgctcctcga tgagccggcc cgccaaagcc tcgtcccaag
     1681 cctgcttgcg cgtcatcgtc tccctctatc aaacggcgct tttgcaactc tcgccggccg
     1741 cgccccggct atagaatcga tcaaaattgc tcgcagaagc aaattgaacg tttcgaccga
     1801 attatagtaa ttttctatca aacgggctcc gccgccgaga ccggcggcac gatgagactg
     1861 ccgatatcgg ccggccgctc catggcgaaa aggttgcggg cgagcggcga gggcggctcg
     1921 cggtcggcga tgatcagacc gatcgtccgc tgcgccttgg gctcgacgag ctcgatcgcc
     1981 ttggccccgc gcggcacgcc gaaaaattgc aggagctgca ccggcacgat cgacgaccac
     2041 aggccggccg agacatgcga gcagagattg aagatcgaat tcgtctccat ggccggcttg
     2101 ggcgacgcgt cggccgagcg aaagatgccg tcgacgatgc ggcggttctg catgtcgggc
     2161 gtcagcaggc agagcggcgt tccgccggct tcgctccagg tgatcgagcg gcgtcgcgcg
     2221 taagggccgg aagacggcgt caggaacatg tattgctcga catagaccgg cttggcgcgg
     2281 acattgctca gcggctcgtt gtcgagatag gtcagcccga catcaagctc gaaatcgtcg
     2341 atcccctgct gaatctgctg cgagttcagc gacaggatgg tgaccagcac gttgggaaaa
     2401 cgctgataga acggcgcggt gatatgggcg atgatcggca gcgccgtcgg gatcgcgccg
     2461 atccgcagcc gcccggaaag gccgcggtcc atttcttcga gatcgcgctt caaggcgccg
     2521 gcctcggcaa ggatgcggtg ggcgtggtcg agagcggctt tcccttgcgc ggtcaggccg
     2581 tggaatttgt ggccgcgctc gacgatcggc gcgccaagct cttcctcaag ctggcggatc
     2641 gcggccgaga gcgtcggctg cgatacatgg cacgccgccg cggcgcggcc gaaatgcttc
     2701 tctcgcgcca aggcgacgag atattcgagc tggcgcagca tcatcggggc gctcctcccg
     2761 gagatgaaag ccggatgata ggctgtcgct atcggatcag gcgccgtcca gccgcgtcaa
     2821 gtccgctgag gccacatctt tcgaggcgag ccaaacgata ggcccgaagt atggaatgcg
     2881 gcgccggcgc gccccggcga tgtcgcggaa gagcgcaatc cgccattgtt tggaacgca
//
LOCUS       DATGJW010000064         2073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1256680, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000064 DATGJW010000000
VERSION     DATGJW010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2073
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1256680"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VKU03_01910"
     CDS             <1..980
                     /locus_tag="VKU03_01910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013895417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ70042.1"
                     /translation="DPSGAVLYANPAAEQIFGHSKDELLNIPLGRPVSAGEIAELTVR
                     RPGKKPAEVEMRVVEFSWDGQPAILASLRDVSARRAHEERRRQSQKLEAIGRLSAGLV
                     HDLNNLLGVFDAGLRLLRKQIAEDPADPKIDMLIEEMLKRAKSGGALTQQLLAFSRRQ
                     PLTPVAVDLNARMESLTNLLKQTLGGGVQIETRLDPALGPVLIDANQFDAALLNLAAN
                     GRDAMGGQGVLTIETCGAPSDLEDLPSAGAGYVRVTVADSGSGMSEDVLAQAFEPFFT
                     TKGEGDGTGLGLSQVYGFVRQSGGQVRIDSEVGTGTRVHLFLPRAPAES"
     gene            1070..1519
                     /locus_tag="VKU03_01915"
     CDS             1070..1519
                     /locus_tag="VKU03_01915"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ70043.1"
                     /translation="MSEYGKLRVLIVEDELLIAMELESCLALLGQEVIGIAAESGEAL
                     ELARTASPDLAVVDLHLRDGCTGPQIAADLVHRHKVLVVFSTGTPHHIPSDYAGALAA
                     ITKPWSPEAVEHVVRFVHAYRAGDRDAMAAPLLPGISIAPSLRETLL"
     gene            1596..>2073
                     /locus_tag="VKU03_01920"
     CDS             1596..>2073
                     /locus_tag="VKU03_01920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLJ70044.1"
                     /translation="MAGTALEHDAGVEPLIERARRNDRAAIRAIIKQQNRRLYRIARS
                     IVGDDSEAEDVLQEAYIRAFRDLGAFRGEAQFGTWLARIVINEALGLLRRRKPVIMLG
                     EAGESAAREAEIIPFPNANPDPETAMAQREIVALLERAIDRLPEPFRAVLVARLVEG"
BASE COUNT          363 a          707 c          665 g          338 t
ORIGIN      
        1 tcgatccgtc cggcgccgta ctctacgcca atcccgccgc cgaacagatt ttcggccatt
       61 cgaaggacga gttgctcaac atcccgctcg gccgccccgt ctccgccggc gaaatagccg
      121 agctgacggt acgccggccg gggaaaaagc cggccgaagt ggaaatgcgc gtcgttgaat
      181 tttcctggga cggccagccg gcgattctgg cgagcctgcg cgacgtctcg gcgcgccgcg
      241 cccacgaaga gcgccgacgc cagtcgcaaa agctggaggc gatcggccgg ctctcggccg
      301 gcctcgtcca tgacctgaac aatctgctcg gcgtcttcga tgcgggcctg cgccttctgc
      361 gcaagcagat cgcggaagat ccggccgatc cgaaaatcga catgctgatc gaggaaatgt
      421 tgaagcgggc gaagagcggc ggcgcgctca cccagcaatt gctggctttc tcgcgtcggc
      481 agccgttgac gccggtcgcc gtcgatctca acgcgcgaat ggaatccttg accaatcttc
      541 tcaagcagac gcttggcggc ggcgtccaaa tcgaaacgcg gctcgatccc gcgctcggcc
      601 cggttctcat cgacgccaat cagttcgatg ccgccctcct caacctcgcc gccaatgggc
      661 gtgacgcgat gggcggccaa ggcgtgctga cgatcgagac atgcggcgcg ccgagcgacc
      721 tcgaagactt gccgagcgcg ggcgccgggt acgtacgggt caccgtggcc gatagcggct
      781 cgggaatgag cgaggacgtg ctggcccaag ctttcgagcc gttcttcacg acaaagggcg
      841 aaggcgacgg gaccggtttg ggcctcagcc aggtctatgg cttcgtccgc cagtccggcg
      901 gccaggtccg catcgacagc gaggtcggaa ccggaacccg cgtccacctc ttcctgccgc
      961 gcgcgccggc ggaaagctag ccgccggcgc ttcttccgca actccaggtg gaaccaacgg
     1021 ctcgccgagc cgttggttcg gaccggatcg aagctccgcg gattggacga tgagcgaata
     1081 cggcaagctg cgcgttctca tcgtcgagga cgagcttctc atcgcgatgg agttggaatc
     1141 ctgcctcgct ttgcttggcc aggaggtgat cggcatagcg gcggaaagcg gcgaagcgct
     1201 cgaactcgca aggaccgcct cgccggacct tgcagtggtc gatctgcatc tccgcgacgg
     1261 ctgcaccggt ccccagatcg ccgccgatct cgtccatcgc cataaagtcc tcgtggtttt
     1321 ctccactgga acgccgcacc atattccgag cgattacgcc ggcgcgctgg cggcgatcac
     1381 caagccgtgg agcccggaag cggtggagca cgtggtccgc ttcgtgcacg cctaccgcgc
     1441 cggcgatcga gacgcgatgg cggcgcccct gctccccgga atcagcatcg ccccgtcctt
     1501 gcgggagacg ctcctctaaa ccgctctcgc ctggcggccg ggaacctttt cggcgagatt
     1561 gcatctaaag cctgtcgaac gcgaaaggga tggcgatggc cgggacagcg ctggagcacg
     1621 atgcgggcgt cgagccgctg atcgagcggg cgcggcgcaa cgatcgcgcc gcgatccgcg
     1681 ccattatcaa gcagcagaat cgtcgcctct accggatcgc gcgcagcatt gtcggcgacg
     1741 acagcgaggc cgaggacgtg ctgcaggaag cctatatccg cgcctttcgc gacctcggcg
     1801 cattccgcgg cgaggcgcaa ttcggcacat ggctcgcccg catcgtgatc aacgaggcgc
     1861 ttggcctcct gcgccgccgc aagcccgtca tcatgctcgg cgaagccggc gagagcgcgg
     1921 cccgcgaagc ggagatcatc ccgtttccca acgccaatcc cgatcctgag acggccatgg
     1981 cccaacgcga aatcgtcgct ttgctcgaac gggccatcga ccggcttccc gagccgtttc
     2041 gcgccgtgct ggtcgcccgt ctcgtcgaag gcc
//
LOCUS       DATGJW010000065         3947 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_323307, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000065 DATGJW010000000
VERSION     DATGJW010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3947)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3947)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3947
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_323307"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..217
                     /locus_tag="VKU03_01925"
     CDS             <1..217
                     /locus_tag="VKU03_01925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="YbhB/YbcL family Raf kinase inhibitor-like
                     protein"
                     /protein_id="HLJ70045.1"
                     /translation="GAVQSGDDAGVKGYFGPCPPKGDKPHHYHITVFAVDVDKLDADQ
                     NSTAAVVGFNLHFHTLAKGVLTGLYGR"
     gene            545..1546
                     /locus_tag="VKU03_01930"
     CDS             545..1546
                     /locus_tag="VKU03_01930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HLJ70046.1"
                     /translation="MVLSVASLAGCAQAPTPRHLSSREHFSPAIYGKASPRLVADGQP
                     VPRGGGVYLVGHAYRVAGHTYVPRENDNYTAIGMASWYGDAFHGRRTANGEIYDKDSI
                     SAAHPTMPLPSYARVTNLSNGRSIIVRVNDRGPFHGGRVMDVSSRVADALEFKGAGTA
                     RVKVEYVGRAPLEGSDDEKLLASLRTDGAPATLDGFPSSAPVMVAGAAMAPVEKLASV
                     FSPAPPPAHRRPPPESPPSPDMVATESQPMRPTPVDLPVPPARPFDLGTIPGAAVPVA
                     FTGPAILPPRRPNSHNQSADNALYFADSGKIRLALDRKGAPFAKLRPQRFEPLSEAR"
     gene            1631..2824
                     /locus_tag="VKU03_01935"
     CDS             1631..2824
                     /locus_tag="VKU03_01935"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HLJ70047.1"
                     /translation="MRTSFSVWRILSAFVACLLVVVAPGGAAEAQSFQSIAPFAYLMD
                     FDSGAALYEKNPDATMAPASMSKLMTLELVFRELKEGRLKLDDEFEVSENAWRTGGAP
                     SHGSAMFLAVHSKARVEDLIRGVAVESGNDAAITLAEGIAGTEQNFTAMMNKRAGELG
                     LTHCSFANPWGKPDPHERVTAHELALLAAHLIRDYPEYYHYFGEKEFTWNRVRQLNRN
                     PLLNMDIGADGLKTGETAESGYGLVGSAVQNGQRLIVVVNGLKTAQERAEEARKLLQW
                     GFRSFDPRTLFQAGDVVGSAEVYGGVKGGVALISPRPITVFLTHGSDERLTAKIVYQG
                     PLVAPVEKGVQVARLKVWRGQTQILDAPLETGEAVGVGTLQQRALDAGFELALELLRK
                     PFTKN"
     gene            2824..3492
                     /gene="tmk"
                     /locus_tag="VKU03_01940"
     CDS             2824..3492
                     /gene="tmk"
                     /locus_tag="VKU03_01940"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HLJ70048.1"
                     /translation="MARKGRFITFEGGEGVGKSTQVKLLAEKLAEAGIAAITTREPGG
                     SPRAEELRRVILSGAAASFGPAGEAILFSAARIDHIDQKIAPALAAGEWVLCDRFADS
                     TRAYQGAAGKLDASLIASLERVAIGETRPDLTFVLDMPVEKSLQRAAERRAKAIKPDR
                     FEGEARAFHETLRRAFLDIAAAEPQRCVVVEADRPSEEVARDIWRLVRERLLDERRRA
                     EVET"
     gene            3489..>3947
                     /locus_tag="VKU03_01945"
     CDS             3489..>3947
                     /locus_tag="VKU03_01945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610822.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta'"
                     /protein_id="HLJ70049.1"
                     /translation="MSRIARDSAPESDAYEGAPHPRETLGLVGHAAAEAELLNAYRAG
                     RLAHAWLIGGPAGIGKATLAWRFARFILAHPDPSAPDVKAARDLSVSDAAPAVRQILA
                     QAHPDVAALRRLWNADRKTFYADIRVEDVRRALQLFQKSAGAGGWRICIVD"
BASE COUNT          652 a         1331 c         1296 g          668 t
ORIGIN      
        1 gggcgcggtg cagtccggcg acgacgcagg cgtgaaaggc tatttcgggc cgtgtccgcc
       61 aaaaggcgac aaacctcacc attatcacat cacggtcttt gcggtggacg tcgacaagct
      121 cgacgcggat caaaactcca ccgccgccgt ggtgggtttc aatctccact tccatacttt
      181 ggcgaagggc gtcttgaccg gcctttacgg ccgctgagcg agcagggcgg gcctcaaatt
      241 caggttttgc gctggctcgc cctctttgtc ctgccgcggc ccgccatatt tgcgtccagc
      301 ttgtggcgct ggtattttgc ctgacaattg acgccaaaaa ggcgttagga ctccggaaag
      361 gaagcagggg caaattgacc atagccgtgt cgcatgtgca cgcccgccgc aattcggcgc
      421 gcgcgtattc cgccgtcgca tgcggcggag cgtgtcagtt tgcgtcgggt atgcgtcgat
      481 gatcaaagcc atccgatggc cgggcgggaa tccatcggcg cggcgcgccc tcaaagccgg
      541 ggcgatggtt ttgagtgtcg caagccttgc cggctgcgcg caggccccga ccccgcggca
      601 tctgagttcg cgcgagcatt tttctccggc gatctatggc aaggcgagtc cgcgcctggt
      661 ggcggacggg caaccggttc cgcgtggcgg cggcgtttat ctggtcggcc acgcctatcg
      721 cgtcgccgga cacacttacg tgccgcgcga aaacgacaat tacacggcga tcggcatggc
      781 gtcctggtac ggcgacgctt ttcacggccg ccgcaccgcc aatggcgaga tttacgacaa
      841 ggattcgatt tccgccgcgc acccgacgat gccgctgccg agctacgccc gggtcaccaa
      901 tctcagcaat ggccgctcga tcatcgtccg cgtcaatgat cgcgggccgt tccatggcgg
      961 ccgcgtcatg gacgtttcgt cccgcgtcgc cgatgcgctc gagttcaagg gcgccggcac
     1021 ggcgcgggta aaggtcgaat atgtcggccg ggcgccgctc gaaggctccg atgacgagaa
     1081 gctgctggcg agcctgcgca ccgacggcgc tccggcgacg ctggacggct tcccgagcag
     1141 cgctccggtc atggtcgccg gcgccgcgat ggcgcccgtc gaaaagctcg ccagcgtgtt
     1201 ctcgccggcg ccgccgcccg ctcatcgccg gccgccgccc gaaagccctc cgtcgcccga
     1261 catggtcgcg acggaaagcc agccgatgcg cccgacgccg gtggatttgc cagtcccgcc
     1321 ggcgcgcccc ttcgatcttg ggacgattcc gggcgcggcc gtccccgtcg cctttaccgg
     1381 cccggccatt ctgccgccgc gacgcccgaa cagccataat cagtccgccg acaacgcgct
     1441 ctatttcgcc gacagcggca agatccggct cgccctcgat cgcaaaggcg cgcctttcgc
     1501 gaagctgcgg ccgcaacgct tcgaaccgct gagcgaggcg cgctgaagcc ctcgccgccg
     1561 ttgaattgcc gcatccaacg ctccacatga caaaggctgg aaagcgagcg ggtcgcttcg
     1621 gctcgaggcg ttgaggacgt cgttttcggt gtggcgcatc ctttcagcgt tcgtcgcctg
     1681 tctcctcgtc gtcgtcgcgc ctggcggcgc ggctgaggcg cagagctttc aaagcatcgc
     1741 gcccttcgcc tatctgatgg attttgacag cggcgcggct ctctacgaga aaaatcccga
     1801 cgccacgatg gcgccggcct ccatgtccaa attgatgacg ctggagcttg tctttcgcga
     1861 gttgaaggaa ggccggctga agctcgacga cgagttcgaa gtgtccgaaa acgcctggcg
     1921 caccggcggc gcgccgtcgc atggctcggc gatgtttctg gcggttcata gcaaggcgcg
     1981 ggtcgaggac ctgattcgcg gcgtcgccgt cgaatccggc aatgacgcgg cgatcacgct
     2041 tgccgagggg atcgccggca ccgagcagaa cttcaccgcc atgatgaaca agcgcgccgg
     2101 cgagctcggc cttacccatt gcagcttcgc caatccttgg ggcaagccgg acccgcatga
     2161 gcgcgtcaca gcgcatgaat tggcgctgct cgcggcgcat ctcatccgcg actatcccga
     2221 atactatcat tatttcggcg agaaggagtt cacctggaac cgggtgcgcc agctcaaccg
     2281 caatcctctg ctcaacatgg atatcggcgc ggacggcttg aaaaccggcg aaacggcgga
     2341 gagcggctac ggcctggtcg gctcggcggt acaaaacggg cagcgcctga tcgtcgtcgt
     2401 caacggcctc aaaacggcgc aggagcgcgc cgaggaggcg cggaaattgc tgcaatgggg
     2461 cttccgctcc ttcgacccgc gcacgctctt tcaagctggc gacgtggtcg gctcggccga
     2521 agtctatggc ggcgtcaagg gcggcgtggc tttgatctcg ccgcggccga tcaccgtgtt
     2581 cctgacgcat ggctcggacg agcgcctgac cgccaagatc gtctatcaag gcccgctggt
     2641 cgccccggtc gaaaagggcg tgcaagtcgc tcgcctcaag gtctggcgcg gccagacgca
     2701 aattctcgac gcgccgctcg aaaccggcga agcggtcggc gtcggcacgc tgcagcagcg
     2761 cgcgctcgac gccggattcg agttggcgct tgaattgctg cgcaagccgt ttacgaaaaa
     2821 ctgatggcca gaaaaggccg cttcatcacc ttcgaaggcg gggagggggt cgggaaatcg
     2881 acccaagtca agctgctcgc cgagaagctc gccgaggccg gaatcgccgc gatcacgacg
     2941 cgcgagccgg gcggctcgcc gcgcgccgag gagctgcgcc gcgtcatcct ctcgggcgcg
     3001 gcggcgtcgt tcggacccgc cggcgaggcg atcctttttt cggccgcgcg gatcgaccat
     3061 atcgatcaga aaatcgcgcc ggccctcgcc gccggcgagt gggtgctgtg cgaccgcttc
     3121 gccgattcga cgcgcgccta tcagggcgcg gccggcaagc tcgatgcgag cctcatcgcc
     3181 agcctggagc gcgtcgcgat cggcgagacg cggccggatt tgaccttcgt gctcgatatg
     3241 ccggtcgaga aaagcctgca acgggcggcc gaacgccgcg cgaaagcgat caagccggat
     3301 cgcttcgagg gcgaggcgcg cgcgttccat gaaacgctcc gccgcgcgtt tctcgacatc
     3361 gccgccgccg agccgcagcg ctgcgtcgtc gtcgaggccg accggccgag cgaagaggtg
     3421 gcgcgcgaca tatggcgtct ggtgcgcgag cggctcctcg acgagcgccg gcgggcggag
     3481 gtcgagactt gagccggatc gcccgcgact cggcgccgga aagcgatgct tatgagggcg
     3541 cgccgcatcc gcgcgaaacc ctcggccttg tcggtcacgc cgccgccgag gcggaactgc
     3601 tcaacgccta tcgcgccgga cggctcgccc atgcctggct gatcggcggc cctgccggaa
     3661 tcggcaaagc cactctcgct tggcgcttcg cccgcttcat cctcgcccat cccgatccgt
     3721 ctgcgccgga cgttaaggcc gcgcgcgatc tttccgtcag cgacgccgcg ccggcggtgc
     3781 gccaaatcct cgcccaggcc catccggatg tcgccgcgtt gcgccgcctg tggaacgccg
     3841 accgcaagac gttctatgcc gatatccgcg tcgaagatgt ccgccgcgcg ctgcaattgt
     3901 ttcagaaatc ggcgggggcg ggcggctggc gcatctgcat cgtcgat
//
LOCUS       DATGJW010000066        13789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1049733, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000066 DATGJW010000000
VERSION     DATGJW010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 13789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13789
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1049733"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            3..560
                     /locus_tag="VKU03_01950"
     CDS             3..560
                     /locus_tag="VKU03_01950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLJ70050.1"
                     /translation="MGDQAADALVERPVSLVEVAGYVKWFDVGKGYGFIVPDDGQPDV
                     LIHVTVLRRDGFQALHEGSRVVAEAVQREKGLQVFRVISVEPADGSHPSQLPPARTHV
                     QVTPTSGFEIVIVKWFNRVRGFGFLSRGEGTEDIFVHMETLRRYGFTEIKPGDTLLAR
                     FGAGPKGLMAAEVRPLESSRAFSSH"
     gene            733..1209
                     /locus_tag="VKU03_01955"
     CDS             733..1209
                     /locus_tag="VKU03_01955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF192 domain-containing protein"
                     /protein_id="HLJ70051.1"
                     /translation="MSLALSSPNGGFWRVLVASLAFILLFYPRAFAQSLDKLEIVSAN
                     GPHVFNVEVVDDEAGRERGLMYRRYMPQDRGMLFDFKQEAPVAFWMKNTYIPLDMIFI
                     SRRGVITKIVANAEPLSERLIPSGGPVYGVLEINGGVAARLGVKPGDRVRDSIFKP"
     gene            complement(1232..3352)
                     /locus_tag="VKU03_01960"
     CDS             complement(1232..3352)
                     /locus_tag="VKU03_01960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004677535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLJ70052.1"
                     /translation="MNSETSSSSERDARLSAEIERRIAVTPAMLHSIDERGRIISVSD
                     LWLAKLGYERDEVIGRPSTDFLTPESRERAARFVLPEFFKTGRCDNVEYQMVRKDGGV
                     IDVLLSGVLDFDPVRNAPVSLAVITDVTSLKIAERKLTESEARYRGLVEDQSEFLSLA
                     APNGDLIYVNPAYANLYGLTPGELIGRNIFEFIPPEAHAQVKEHFRFVCTVSHSVESR
                     NQALLPNGERRWTSWTNRALRDAQGAVTAIHSVGRDIEEWVRADKLLQESESRYRFLA
                     ENTADMILLVGLDGRRHYASPACRALLGYEPEEMLQIRTKDAIHPDDAAKVLQALAGA
                     STGASLTYRMRRKDGGYVWVETSGRLVNFSDSEPKRLVIVRNIEQRVAAEQRLRESEA
                     RYRLLADNSTDVVLLLDLDLSRRYVSPACREIFGYEPEELLGAKTGGMAHPDDTAQVA
                     QVLKSLLSGQADRQTVVARRRHRDGKWIWIEAKYRLLRDAETGSPIGIVGTIRDISSR
                     KAIEEQLAEAYRRLEVLAREDGLTGLANRRVFDDSLVREFRRAYRAKRSLALIMIDVD
                     QFKPFNDQYGHPAGDECLRLISGAIKVTTRRPGDLATRYGGEEFAVLLPDTDESGASE
                     VAERIRRAVAELSLQHAGVRIKCTTISAGVAAVTPQKFGGSGAAAALVDAADRALYRA
                     KRAGRNAVIRASAEDSSTKSSSAA"
     gene            complement(3663..4127)
                     /locus_tag="VKU03_01965"
     CDS             complement(3663..4127)
                     /locus_tag="VKU03_01965"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ70053.1"
                     /translation="MAADLDALIRLNEAIQSVHANLYPKQFKPRPNPASLRTFFAARL
                     TEIVLAELEGLPVGYVWCEIQSRPETPFSPGRPRLYVHHLSVEPQARRRGVGAALMRY
                     VEQEAIQRGVAEIALDVWEENDEALAFFGTQGFAKLIVTLHKTLETPPPPAC"
     gene            4273..5403
                     /locus_tag="VKU03_01970"
     CDS             4273..5403
                     /locus_tag="VKU03_01970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ppx/GppA phosphatase family protein"
                     /protein_id="HLJ70054.1"
                     /translation="MEAKVRPASGRARGVRALALIAPLGAPAEARAALGDLSRPRRAG
                     GGAPAYAALDLGTNNCRLLVARPTHDGFRVVDAYSRIVRLGEGLSQTGRLSQAAIERT
                     MAALAVCRDKMRARGVSRARLIATEACRAAENGAEFLDEVRERLGIHLEIVDRETEAR
                     LAVVGCGALVDRDAKSTVLFDIGGGSSEVVWLAGGEGRAGGFAEPGVKAWESLRLGVV
                     TLAERFGGVDVDAGVFAAMVEFVAERLAPFAARVDGEARCERFHLLGTSGTVTTIAGV
                     YLDLPRYDRRYVDGLWMNRIAIDETVGRLRAMSYAERVANPCIGRERADLVLAGCAIL
                     EAIRLSFPAERLRIADRGLREGILTQLMSADRVWRQANGAEA"
     gene            5400..6119
                     /locus_tag="VKU03_01975"
     CDS             5400..6119
                     /locus_tag="VKU03_01975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RlmE family RNA methyltransferase"
                     /protein_id="HLJ70055.1"
                     /translation="MKSAKDGPGRSGREGGRSLKSRVRTADKRSLSSTLWLERQLNDP
                     YVAAARRQGYRSRAAFKLIEIDDKYRLLKRGQNIVDLGAAPGGWSLVAAERVGAASGE
                     GRVVGIDLLDIEPIAGVDFKVMDFHDPQAPAFLKETLGGKADGVLSDMAANATGHRKT
                     DHLRIVALAELAADFAREVLAPGGFFLAKVLQGGAEGALLADLKRDFATVRHVKPKAS
                     RADSAELYVLATGFRGAPAKD"
     gene            complement(6300..7718)
                     /locus_tag="VKU03_01980"
     CDS             complement(6300..7718)
                     /locus_tag="VKU03_01980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0006855 - xenobiotic transmembrane
                     transport [Evidence IEA];
                     GO_process: GO:0009410 - response to xenobiotic stimulus
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DHA2 family efflux MFS transporter permease
                     subunit"
                     /protein_id="HLJ70056.1"
                     /translation="MPRSLLTPLIIATALLMENLDGTVISTALPAIAHDFGLDPIVLK
                     LALTSYMLTLAVFIPASGWVADRFGGRTVFCAAIIVFTLGSICCGASNSLRTLIAARI
                     FQGVGGAMMVPVGRLVLLRSVPKKELVSALAYLTIPAMLGPVLGPPVGGFITTYFHWR
                     WIFWINVPIGLIGLTLAFAFIDNIREEEVAPFDYTGFFLSGFGLAAVMFGLTVIGRNF
                     TSLPTELAILAVGATAITLYVRHAKSEPNAILDLSLLKTPTFFAGVVGGFIFRIGVGA
                     IPFLLPLMLQVGFGLTPFASGSLTFAAAAGSMAMKFSATSIIRRFGFRRILVVNAFIS
                     GLFLASYGLFTHATPQSLIFVALLVGGFFRSLEFTALNAIGFADIEQARMSRATSFSS
                     VAQQMSQTTGIAAGAAAVEGIQYLYGDADLLARDFGAAFFIVALISTGSAAIFARLAP
                     NAGAEVSGRRARRSESAPAAAE"
     gene            7992..9074
                     /gene="recA"
                     /locus_tag="VKU03_01985"
     CDS             7992..9074
                     /gene="recA"
                     /locus_tag="VKU03_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580602.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008094 - ATP-dependent activity, acting
                     on DNA [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase RecA"
                     /protein_id="HLJ70057.1"
                     /translation="MTQANLRLVEGTSVDKTKALDAALSQIERAFGKGSIMRLGKNQQ
                     AVEIETISSGSLGLDIALGVGGLPRGRVIEIYGPESSGKTTLTLHVIAEAQKRGGVCA
                     FVDAEHALDTVYARKLGVNLEDLLVSQPDTGEQALEITDTLVRSGAVDVLVVDSVAAL
                     TPRAEIEGEMGESQPGLQARLMSQALRKLTASISRSNTMVIFINQIRMKIGVMYGSPE
                     TTTGGNALKFYASVRLDIRRIGAIKDREEVVGNQTRVKVVKNKVAPPFKQVEFDIMYG
                     EGVSKSGELIDLGVKAGVVEKSGAWFSFDSQRLGQGRENAKAFLKANPDIADKIERAI
                     RENAGLIAERILDNTDPAELDDAADA"
     gene            9461..12124
                     /gene="alaS"
                     /locus_tag="VKU03_01990"
     CDS             9461..12124
                     /gene="alaS"
                     /locus_tag="VKU03_01990"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HLJ70058.1"
                     /translation="MNGVNEIRASFIDYFAKHGHEAVASSSLVPRNDPTLMFTNAGMV
                     QFKNVFTGVEKRAYKRAVTAQKCVRAGGKHNDLDNVGYTARHHTFFEMLGNFSFGDYF
                     KPLAIELAWNLITKEFGLAKDKLTVTVYHDDDDAFDLWKRVAGLPESRIVRIATSDNF
                     WAMGDTGPCGPCSEIFYDHGDTIPGGPPGSADADGDRFIEIWNLVFMQFETLPGGERI
                     NLPRPSIDTGMGLERIAAVLQGVHDNYDIDLFRALIRASIEATGVDDEGARKASHRVI
                     ADHLRASSFLVADGVLPSNEGRGYVLRRIMRRAMRHAQLLGAKEPLMSRLVPTLVREM
                     GQAYPELVRAEALIAETLKLEETRFRAALARGLSILDEETKALAPGASLSGEIAFKLY
                     DTYGFPLDLTQDALRARGLGVDTEGFSAAMERQRAEARKAWAGSGEAATETIWFSLRE
                     KLGATEFLGYETEEAEGVALAILKDGADVERLAAGEKGAVILNQTPFYAESGGQVGDT
                     GAILGGSLRARVTDTHKKLGDLFVHDVTVEEGALAVGKPLELKVDHERRAAIRANHSA
                     THLLHEALRIALGEHVAQKGSLVAPDRLRFDIAHPKPITKTELAQVEDIANRIVLQNE
                     PVVTRLMAVDEAVASGARALFGEKYGEEVRVVSMGAADGANGPRPYSVELCGGTHVAR
                     TGDIGLISIIGESAVAAGVRRIEAKTRDGARRRLNEEAAALAEVAEKLRAPLAEVGAR
                     LDALLDERRKLERELSDAKKKLAMGGGSAGGNPVRDVAGVKVYARVVSGVEMKDLKSL
                     ADEAKGGVGSGVVAIVALGEDGKASVVVGVTNDLTQRFNAVDLVRIASATLGGKGGGG
                     RPDMAQAGGPDGAKAEAALSAIEEALRKKAA"
     gene            complement(12183..12713)
                     /locus_tag="VKU03_01995"
     CDS             complement(12183..12713)
                     /locus_tag="VKU03_01995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4188 domain-containing protein"
                     /protein_id="HLJ70059.1"
                     /translation="MRTRVERQTVDLSGYPDLVVIYLGMRVNRLAGIKTLLGFGPKIS
                     ASVAEQPDGLLRHETIIYSLFPMHIGMRQYWRDPGSLLAWTRSEPHRQWWRNFLSDSG
                     GTGFWHEAYFRRGGFEAIYDDVPQPLGFMSFAPLAAARGPMFGAAARAGKASPGEPVL
                     SEEELYGGADAAPRGT"
     gene            12860..>13789
                     /locus_tag="VKU03_02000"
     CDS             12860..>13789
                     /locus_tag="VKU03_02000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004180 - carboxypeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLJ70060.1"
                     /translation="MLTEELKRYEPDLVAIRHDIHRHPETGFEEHRTAKLVAARLREW
                     GVDVAEGLAKTGIVGTLRGARPGQKAIGLRADLDALHISEKTGRDYASEISGKMHACG
                     HDGHTAMLLGAARYLAAHRDFAGVVHFIFQPAEEGLGGGRLMVEEGLFERFPVDAVYG
                     MHNSPGLPVGKFATRVGPFLSASDTWTVTFRGTGGHGGAGAHRAKDPTIPQAHFVLAL
                     QTIVGRSVPAVETAVVSVGSIAGGDHASPNIIPAEIVVRGTARSYKPAIRDLIEKRLG
                     ELAHALAAAYGCVAEVEYERRYPPLITHAEETAT"
BASE COUNT         2395 a         4517 c         4551 g         2326 t
ORIGIN      
        1 agatcggcga tcaagccgcg gacgcgctgg ttgaaagacc ggtcagtctt gtcgaggtcg
       61 ccggctatgt gaaatggttc gacgtcggca agggttacgg tttcatcgtg cccgacgacg
      121 gccaaccaga cgtgctcatt cacgtcacag tgttacgtcg cgacggcttc caggccttgc
      181 atgagggctc gcgggtcgtc gccgaggcgg tgcagcgcga aaaaggtttg caggtcttcc
      241 gggtgatttc ggtcgagccg gccgacggct cgcatccctc gcaattgccg cccgcccgca
      301 cccatgtcca ggtgacgccg accagcggct tcgaaatcgt gatcgtcaaa tggttcaacc
      361 gggtgcgcgg cttcggcttc ctgtcccgcg gcgaaggcac cgaggacatt ttcgtgcata
      421 tggagacctt gcggcgctac ggcttcaccg agatcaagcc gggcgacacg ctgctggcgc
      481 gcttcggcgc cggcccgaaa gggttgatgg ccgcggaggt gcggccgctc gaaagctcgc
      541 gcgctttctc ctcgcattga agctggcgcg gcggctctgc gtcgaagcgg gcgctcgccc
      601 gctctaatgg ccggatttcc tggacatcgc cgctcccggt tccgtcgcaa ggccgcctca
      661 ttcctctttc atggagccgc gcagcgcttt aaaagcgtag cgcggcgaag ttcgtgaatg
      721 tcgaggatcg ccttgtctct tgccctctcc agcccgaacg gcgggttttg gcgcgttctc
      781 gtcgcatcgc tcgccttcat tctccttttt tatccgcgcg ccttcgcgca aagcctggac
      841 aagctcgaaa tcgtcagcgc caacggcccg catgtcttca acgtcgaggt cgtcgacgac
      901 gaggcggggc gtgagcgcgg cctgatgtac cgccgctaca tgccgcaaga ccgcggcatg
      961 ctgttcgact tcaagcaaga ggcgccggtc gcgttctgga tgaagaatac gtacattccc
     1021 ttggacatga tcttcatttc gcgccggggc gtgatcacga aaatcgtcgc caacgccgag
     1081 cctctatcgg agcgcctgat tccgtccggc ggcccggttt atggcgtgct cgaaatcaat
     1141 ggcggcgtcg ccgcccgcct cggcgtcaag cctggcgacc gggtgcggga ttcgattttc
     1201 aagccgtaga atcgcaatgt tctgagcgat ttcaagcggc ggacgagctc tttgttgagg
     1261 agtcctcggc cgaggcgcgg atcaccgcgt tgcggccggc gcgcttggcg cgatagagcg
     1321 cccggtcggc ggcgtcgacc aaagcggcgg cggcgccgct cccgccgaat ttctgcggcg
     1381 ttacggccgc gacgccggcg ctgatcgtcg tgcattttat ccggacgccg gcatgctgga
     1441 gcgacagttc cgcgacggcg cgccggattc gttcggcgac ctcgctcgcg ccgctctcgt
     1501 cggtgtcggg aagaaggacc gcaaattctt cgccgccata tcgggtggcg agatcgcccg
     1561 gccggcgggt cgtcaccttg atcgcgccgc tgatcagccg caggcattca tcgccggccg
     1621 gatggccata ttggtcgttg aacggcttga attggtcgac gtcgatcatg atcagcgcca
     1681 agcttctctt ggcgcgatag gcccgtctga attcccgcac caggctgtcg tcgaagacgc
     1741 ggcgattggc gagaccggtc aggccgtcct ctcgcgccag cacttcgagg cgccgataag
     1801 cctccgccaa ttgctcttcg atcgccttgc gcgaggaaat atcccggatc gtcccgacaa
     1861 tcccgatcgg gcttccagtt tcggcgtctc tcagcaggcg gtatttggct tcgatccaga
     1921 tccactttcc gtcgcggtgg cgccgcctcg caacgaccgt ttgacggtcg gcctggccgg
     1981 acagaaggga tttcaggacc tgagccacct gggccgtatc gtcgggatga gccatcccgc
     2041 cggttttcgc gccgagcaat tcctccggct cgtagccgaa gatttcgcgg caagccggag
     2101 agacgtagcg gcgcgacaaa tcgagatcga gcaacaggac gacgtcggtg ctgttgtcgg
     2161 ccaaaaggcg gtagcgcgct tcgctttccc tcaggcgctg ctccgccgcg acgcgctgtt
     2221 cgatgttgcg gacgatgacc aggcgtttgg gctcgctatc ggaaaaattg accagccgtc
     2281 ccgacgtttc gacccagaca tagccgccgt ccttgcggcg catccggtac gtcaggctgg
     2341 cgcccgtgct cgccccggcc agcgcctgca ggactttcgc cgcgtcgtcc ggatgaattg
     2401 cgtccttggt cctgatttgg agcatctctt ccggctcata gccgagaagc gcgcggcagg
     2461 ccggcgaagc gtagtgtctg cggccgtcaa ggccgacgag gaggatcatg tcggcggtgt
     2521 tttcggccag gaagcgatac cgggattcgc tttcctgaag gagcttgtcg gcccgcaccc
     2581 attcctcgat gtcgcgcccg accgagtgaa tggcggtcac cgcgccttgc gcgtctcgca
     2641 gagcgcgatt ggtccatgac gtccagcgcc gctcgccgtt cggcagcagc gcctggttcc
     2701 tgctttcgac gctatggctc accgtgcaga cgaagcgaaa gtgctccttc acctgcgcgt
     2761 gcgcttccgg cgggatgaat tcgaaaatat ttctgccgat caattcgccg ggcgtcaggc
     2821 catagagatt ggcgtaggcc ggattcacat agatgaggtc gccattgggc gcggcgaggg
     2881 aaaggaactc cgactgatct tcgaccaggc cgcggtagcg cgcttcgctt tcggtgagct
     2941 ttcgctcggc gattttgagc gaagtcacat cggtgatgac ggcgagcgag acgggcgcat
     3001 tgcgtacggg atcgaaatcc aggacgccgg acagcaagac gtcgatgacg ccgccatcct
     3061 tgcgcaccat ctggtattcg acattgtcgc accggccggt cttgaagaat tccggcaaga
     3121 cgaagcgcgc ggcgcgttcg cgcgattccg gcgtcaggaa atcggtcgac gggcggccga
     3181 tcacctcgtc gcgctcatag cccagcttgg cgagccaaag atcgctgacc gagatgatcc
     3241 gtcctctttc gtcgatcgaa tgcagcattg ccggcgtgac ggcgatccgc cgctcgatct
     3301 cggcgctgag ccgcgcgtcc cgctcgcttg aggatgaggt ttcggaattc acattcagcg
     3361 ccgtcgcgac gacatcccgg cccgctttgc cgcatcatcc aggataagtc ggatgaataa
     3421 agaaacctgt cctggttccc gcctatttcc cctttgcgtt aggaactcgt taccaaagcc
     3481 gcttgaattg atcgggtcgc gaatgctccc acgccgaaag gggcggggga cttgcgcgca
     3541 gcgccggacg gctgactccg tttcgagcgc cgcggcccct ccaccatgct tcgcgtgccc
     3601 ccccgttccg cattctctcc agggggagga aagctcggtt cgagctttca agctcgataa
     3661 ggtcaacaag caggcggtgg cggcgtttcg agcgttttgt gcagcgtgac gatgagcttg
     3721 gcgaagcctt gagtcccaaa gaaggcgagc gcctcgtcgt tctcctccca gacgtccagc
     3781 gcaatttcag cgacgccccg ctggatcgcc tcttgctcga cataacgcat caacgccgcg
     3841 ccgaccccgc gtcgccgcgc ttgcggctcg accgacagat gatgcacata aagccgtggc
     3901 cgtcccggtg aaaacggcgt ctctggtcgg ctctgaattt cacaccagac atagccgacc
     3961 gggaggccct caagctcagc tagaacgatt tccgtaagcc gggcggcaaa gaaggtccgc
     4021 aacgaggccg gatttggcct gggcttgaac tgcttggggt aaaggttggc gtgcacgctt
     4081 tgaatcgcct catttaggcg gataagcgcg tccaggtccg ccgccaaggc tcgtcgcact
     4141 gtcgcaggca tcgggtccct cccgccgccc agcctcgaaa cggcgggttc ttctgtcaac
     4201 gcctgcatcg cccggccgct gggaaggggc cgcgtccatc cgtcggttgc gtcgccccgt
     4261 ccggtcgcta caatcgaggc gaaagttcgc ccggcgagcg gccgggcgcg aggagttcga
     4321 gctttggctc tcatcgcgcc gttgggcgcg cccgcagagg cgcgcgcggc tcttggggat
     4381 ttatcgcggc cgcggcgcgc aggcgggggc gcgccggcct atgccgcgct cgatctcggc
     4441 acgaacaatt gccggctgct cgtcgcccgg ccgacccatg acgggttccg ggtcgtcgac
     4501 gcctattcgc gcatcgtccg gctcggcgag ggcctgtcgc agaccggccg gctgagccag
     4561 gcggcgatcg agcgcaccat ggcggcgctc gccgtctgcc gcgacaagat gcgcgcgcgc
     4621 ggcgtcagcc gcgcccggct gatcgccacc gaggcctgcc gcgccgccga gaacggcgcg
     4681 gaattcctcg acgaggtccg cgagcggctc ggcatccatc tcgaaatcgt cgatcgcgag
     4741 accgaggcgc ggctcgccgt ggtcggctgc ggcgccttgg tcgaccgcga cgccaaatcc
     4801 accgtcctgt tcgacattgg cggcggctct tcggaagtcg tgtggctcgc cggcggcgag
     4861 gggcgcgctg gcggattcgc cgagccgggc gtgaaagcgt gggaatcgct gcgccttggc
     4921 gtggtgaccc tcgccgagcg cttcggcggc gtcgatgtcg acgccggcgt tttcgccgcg
     4981 atggtcgaat tcgtcgccga gcgcctggcg ccgttcgcgg ccagggtcga cggcgaggcg
     5041 cgctgcgagc ggttccatct gctcggcacg tccggcacgg tgacgacgat cgccggcgtc
     5101 tatctcgatc tgccgcgcta cgaccggcgc tatgtcgatg gcctgtggat gaaccgcatc
     5161 gcgatcgacg agacggtcgg ccgcctgcgc gccatgtcct acgccgagcg cgtcgccaat
     5221 ccgtgcatcg gccgcgaacg cgccgatctt gtcctggccg gctgcgccat tctcgaagcg
     5281 atccgcctga gcttcccggc cgagcgcctg cgcatcgccg accgcggcct gcgcgagggc
     5341 atcctcacgc aattgatgag cgccgatcgc gtctggcggc aggcgaacgg cgccgaagcg
     5401 tgaagagcgc caaggacggc cccggccggt cgggccgcga aggcggccgc tcgctgaaaa
     5461 gccgcgtgcg caccgccgac aagcgcagcc tttcctcgac cctgtggctg gagcgccagc
     5521 tcaacgaccc ctatgtcgcc gcggcgcggc ggcagggcta tcgctcgcgc gccgccttca
     5581 aactgatcga gatcgacgac aaataccggc tcctcaagcg cggccagaac atcgtcgatc
     5641 tcggcgcggc gccgggcggc tggtcgctgg tcgccgccga gcgcgttggc gcggcctcgg
     5701 gcgagggcag ggtggtcggc atcgaccttc tcgacatcga gccgatcgcc ggcgtcgatt
     5761 tcaaagtcat ggattttcac gatccgcagg cgccggcttt tttgaaagag acgctgggcg
     5821 gcaaagccga cggcgttctc tcggatatgg cggccaatgc gaccggccat cgcaagacgg
     5881 accatctgcg catcgtcgcc cttgccgagc tcgccgccga tttcgcgcgc gaggtattgg
     5941 cgccgggcgg gttctttctc gccaaggtcc tgcaaggcgg cgcggaaggc gcgctgctcg
     6001 ccgatttgaa gcgggacttt gcgactgtgc gccacgtcaa gccgaaagcc agccgggcgg
     6061 acagcgccga gctctatgtg ctggcgaccg ggttcagagg cgcgccggcg aaagactgac
     6121 gtaccctcac cccaaccctc tcccgcaagc gggagagggg gcgcaccagc gtcgcgctgt
     6181 agccgcggtc gacgacctcg gctccaatcc ttctcccgct tgcgggagaa ggtggcgcga
     6241 agcgccggat gagggtgagc cgcggcggct ataaaagtac gattcgccca acgggccgct
     6301 cactccgccg cggccggggc gctctcgctc cgtcgcgctc gtcgtcccga cacttccgcg
     6361 cccgcgttcg gcgcaaggcg cgcgaagatc gccgccgagc ccgtcgagat gagggccaca
     6421 atgaagaagg ccgcgccgaa atcgcgcgcc aagaggtcgg cgtcgccgta aagatactga
     6481 atcccctcca ccgccgccgc gccggcggcg atgccggttg tctgcgacat ttgctgcgcg
     6541 acgctggaga agctggtggc ccggctcatc ctggcctgct cgatatcggc gaagccgatc
     6601 gcgttgagcg cggtgaattc gagcgagcgg aaaaaaccgc cgacgagcag cgcgacgaag
     6661 atcagcgatt gcggcgtcgc atgggtaaag aggccgtacg acgccaagaa caggccgctg
     6721 atgaacgcat tgacgaccag gatccggcga aagccgaagc gccggatgat ggacgtcgct
     6781 gagaatttca tcgccatcga gccggcggcc gcggcgaagg tcaacgagcc cgaggcgaaa
     6841 ggcgtcaagc cgaagccgac ctgcagcatc agcggcagca gaaacggaat ggcgccgacg
     6901 ccgatgcgaa aaataaatcc gccgaccacg ccggcgaaaa aagtcggcgt ctttaaaagg
     6961 ctcaaatcga ggatcgcgtt cggctcgctt ttggcgtgcc ggacatagag cgtgatcgcc
     7021 gtcgcgccga ccgccaggat cgccagctcc gtcggcagcg aggtgaagtt gcggccgatc
     7081 acggtcaagc cgaacatcac ggcggcgagg ccgaagccgg acaggaagaa gccggtataa
     7141 tcgaacggcg cgacctcctc ttcgcggata ttgtcgatga aggcgaaggc gagcgtcagg
     7201 ccgatcagcc cgatcggcac gttgatccaa aaaatccagc gccagtggaa gtaagtggtg
     7261 atgaagccgc cgaccggcgg gccgagaacc gggccgagca tggccgggat ggtgaggtaa
     7321 gcgagcgcgc tgaccagctc ctttttcggg acgctgcgca ggagcacgag gcggccgacc
     7381 ggcaccatca tcgcgccgcc gacgccctga aagatgcgcg ccgcgatcag ggtgcgcagc
     7441 gaattggacg ccccgcagca gatcgagccg agcgtgaaga cgatgatcgc cgcgcagaac
     7501 acggtccggc cgccgaaacg gtcggcgacc cagccgctcg ctggaatgaa cacggcgaga
     7561 gtcagcatat aggaggtcag cgccagcttc aggacgatcg gatcgaggcc gaaatcatgg
     7621 gcgatggccg gcagcgccgt cgagatcacc gtcccgtcga gattctccat cagcaaagcg
     7681 gtcgcgatga tcagcggcgt cagcagggag cggggcacgg cgaaaccttt ccgcgtcctt
     7741 gtggcgccgc atggcgaggc tcgcaatggc cggcggcccg gaaaaaccgg gttggcgagg
     7801 cgggcggcgc gcacgaatcg gtgaacgagc ggcggcgaat ggagcgccgc gccgagcaat
     7861 ttgccggcct ctcgcaattc gaacgcaaca ggaacaaaaa tcgcttgcgc atgagaacaa
     7921 aacatgcaca tattgtcgtc cgaggccgct gttgcggcct cgccgccgac ctgtcagaag
     7981 gatgacgcgc cgtgacgcaa gccaatctcc gcctcgtgga agggacctcc gtggataaga
     8041 ccaaggcgct cgacgccgct ttgtcgcaaa tcgagcgggc cttcggcaaa ggctcgatca
     8101 tgcggctcgg caagaaccag caggcggtgg agatcgagac gatttcgtcc ggctcgctcg
     8161 ggctcgacat cgcgctcggc gtcggcggcc tgccgcgcgg ccgggtgatt gaaatttatg
     8221 ggccggaatc gtccggcaag accacgctca ccctgcatgt cattgccgag gcgcagaaaa
     8281 gaggcggcgt ctgcgccttt gtcgacgcgg agcatgcgct ggacacggtc tatgcgcgca
     8341 aactcggcgt caatctcgaa gacttgctcg tctcccagcc cgataccggc gagcaggcgc
     8401 tcgaaatcac cgacactttg gtgcgctccg gggcggtcga cgtgctggtc gtcgattcgg
     8461 tcgcggcttt gacgccgcgc gccgaaatcg aaggcgagat gggcgaatcg cagcccggct
     8521 tgcaggcgcg gctgatgagc caggccttgc gcaaactgac cgcctcgatc tcgcgctcca
     8581 acacgatggt catcttcatc aaccagatcc gcatgaagat cggcgtcatg tatggttcgc
     8641 cggagaccac gaccggcggc aacgccttga aattctacgc ctccgtccgc ctcgacatcc
     8701 gtcgtatcgg cgccatcaag gatcgcgagg aggtcgtcgg caaccagacc cgggtcaagg
     8761 tcgtcaaaaa caaggttgcg ccgcctttca agcaggtcga attcgacatc atgtatggcg
     8821 agggcgtctc caagagcggc gaattgatcg atctcggcgt caaggccggc gtggtcgaga
     8881 aatcgggcgc gtggttctca ttcgacagcc agcgcctcgg ccagggccgc gagaacgcca
     8941 aggcgttcct gaaggccaat ccggatattg ccgacaagat cgagcgcgct atccgcgaga
     9001 atgccgggct gatcgccgag cgcattctcg acaacaccga tccggcggaa ctcgacgacg
     9061 ccgccgacgc ttgaaagagg tcaaccgggc gcttgtcccc cggcgcccgg ccgccgaacc
     9121 ttcgttccag cttttgcggc ttcccgcggc cgcgccccaa gggcgcggcc gcgtttggcc
     9181 gtttaggcgc aggtacgggc gcatcccttc gccccgcgaa gcggggagaa agggtggacg
     9241 cgcgcagcgc ggccggatga ggggctacgc tgaattctgg gatgagcgct tcgtagggcg
     9301 ctcgtttcgt tagtcctgcg ccgcccctca tccggccctt cgggccacct tctccccgaa
     9361 gggattctca ggccatttcc gctttcccat tcgcggcgcg atgttctaga acaggccgct
     9421 ttttcaaggc cccgcggcgc gcggggcagg gcgagggttc atgaacggcg tcaacgagat
     9481 cagggcgagc ttcatcgatt atttcgccaa gcatggccac gaggcggtgg cgtcgagctc
     9541 gctcgtgccg cgcaacgacc cgaccttgat gttcaccaat gccggcatgg ttcagttcaa
     9601 gaacgtcttc accggcgtcg agaaacgagc ttacaagcgg gcggtcaccg cccagaaatg
     9661 cgtgcgcgcc ggcggcaagc acaacgacct cgacaatgtc ggctacaccg cgcgccacca
     9721 caccttcttc gaaatgctcg gcaatttctc gttcggcgac tatttcaagc cgctggcgat
     9781 cgagctcgcc tggaatctga tcaccaagga attcggcctc gccaaggata agctcaccgt
     9841 caccgtctat cacgacgacg acgatgcgtt cgatctctgg aagcgcgtcg ccggcctgcc
     9901 ggaatcgcgc atcgtccgca tcgccacgag cgacaatttc tgggccatgg gcgataccgg
     9961 cccgtgcggg ccgtgctcgg aaattttcta cgatcacggc gacacgattc cgggcggccc
    10021 gcccggctcg gccgatgcgg acggcgatcg cttcatcgag atctggaacc tcgtcttcat
    10081 gcaattcgaa acgctgccgg gcggcgagcg gatcaacctg ccgcggccgt cgatcgatac
    10141 cggcatgggc ctcgagcgca tcgccgccgt gctgcagggc gtgcacgaca attacgacat
    10201 cgatctcttc cgcgcgctga tccgcgcctc gatcgaggcg accggcgtcg acgacgaagg
    10261 cgcgcgaaaa gcctcgcacc gtgtgatcgc cgaccatttg cgcgcctcgt cctttctcgt
    10321 cgccgacggc gtgctgccgt ccaacgaagg ccgcggctat gtgctccgcc ggatcatgcg
    10381 ccgggccatg cgccacgccc agctcctcgg cgcgaaggag ccgctgatgt cgcggctcgt
    10441 gccgactctg gtccgcgaaa tgggccaggc ctatcccgag ctcgtccgcg ccgaagccct
    10501 gatcgccgaa acgctgaagc tcgaagagac gcgctttcgc gccgccttgg cgcgcggcct
    10561 ctccatcctc gacgaggaga ccaaggcgct cgcgcccggc gccagccttt ccggcgaaat
    10621 cgcgttcaag ctctatgaca cctacggttt tccgctcgac ctgacccagg acgccttgcg
    10681 cgcccgcggc ctcggcgtcg acacggaagg cttctccgcc gcgatggagc gccagcgcgc
    10741 cgaggcgcgc aaggcctggg ccggctcggg cgaagcggcg accgagacga tctggttctc
    10801 cttgcgcgaa aagctcggcg cgaccgagtt cctcggctac gagaccgaag aagccgaggg
    10861 cgtcgcgctg gcgattctga aagacggcgc ggacgtcgag aggctggcgg ccggcgagaa
    10921 gggcgcggtc atcctcaacc agacgccgtt ctacgccgaa tctggcggcc aggtcggcga
    10981 taccggcgcg atcctcggcg ggtccttgcg cgcgcgcgtc acggatacgc ataagaagct
    11041 cggcgatctc ttcgtgcatg atgtcacggt cgaggaaggc gcgctcgcgg tcggcaagcc
    11101 gctcgagctg aaagtcgatc acgagcgccg cgcggcgatc cgcgccaatc actcggcgac
    11161 ccatctcctg cacgaggccc tgcgcatcgc gctcggcgag catgtcgcgc agaaaggctc
    11221 gctggtcgcg cccgaccgcc tgcgcttcga catcgctcat cccaagccga tcaccaagac
    11281 cgaactggcg caagtcgagg acatcgccaa tcgcatcgtc ctgcagaacg agccggtcgt
    11341 gacgcggctg atggcggtgg acgaagcggt cgcctccggc gcccgcgcct tgttcggcga
    11401 gaaatacggc gaggaagtgc gcgtcgtctc gatgggcgcc gccgacggcg ccaacggtcc
    11461 gcggccctat tcggtcgaat tgtgcggcgg cacgcatgtg gcgcgcaccg gcgatatcgg
    11521 cctcatctcg atcatcggcg agagcgcggt cgccgctggc gtgcgccgca tcgaagccaa
    11581 gactcgcgac ggcgcgcgcc gtcgcctcaa tgaggaggcg gcggcgctgg cggaagtcgc
    11641 cgagaagttg cgcgcgccgc ttgccgaagt gggcgcgcgg ctcgatgctt tgctcgatga
    11701 gcggcgcaag ctcgaacgcg aattgtcgga cgccaagaag aagctcgcca tgggcggcgg
    11761 ctctgcgggc ggcaatccgg tcagggatgt cgccggcgtc aaggtctatg cgcgcgtcgt
    11821 cagcggcgtc gagatgaagg atttgaaatc gctcgccgac gaagccaaag gcggcgtcgg
    11881 ctccggcgtg gtcgccatcg tcgcgcttgg cgaggacggc aaggcaagcg tcgtcgtcgg
    11941 cgtgaccaac gatttgaccc agcgcttcaa cgccgtcgat ctcgtccgca tcgcctccgc
    12001 gacgcttggc ggcaaaggcg gcggcggccg ccccgacatg gcccaagccg gcggccccga
    12061 cggcgcgaag gccgaggctg cgctcagcgc gatcgaggag gcgctgcgca agaaggcggc
    12121 gtgagcaacc gcgccgcaac gccgcttcgc caagcgcgca agccgaaagc gctttcgtcg
    12181 ggtcacgtcc cgcgcggcgc agcgtcggct ccgccgtaaa gctcttcttc ggataaaacg
    12241 ggctctccgg gggatgcttt gccggccctt gcggctgcgc cgaacatcgg gccacgcgcc
    12301 gccgccaggg gcgcgaagct catgaaaccc aacggctgcg gcacatcgtc atagatggcc
    12361 tcgaagccgc cgcgccgaaa ataggcctcg tgccagaatc cagttccacc cgaatcgctc
    12421 aagaagtttc gccaccattg ccggtgcggc tccgaacgcg tccaggccag cagcgacccg
    12481 gggtcgcgcc aatattggcg catgccgata tgcatgggga aaagcgaata tatgatcgtc
    12541 tcatggcgga ggagcccgtc cggctgctcg gcaaccgaag cagatatttt gggtccgaag
    12601 ccgagcagcg tcttgattcc ggcgaggcga tttactctca tgccgagata aatcaccacc
    12661 aagtccggat agccggaaag atcgacggtt tgccgctcta cgcgcgtgcg catcggaaaa
    12721 ggcgctccga cgggcataat actcctcttt gtcgcccggc aaatcttgtc ggcggcggcc
    12781 gagccgcgcc ggcgccgggt ttcttcgggc aatcttgcct gctgtcgctg gggacggaag
    12841 ggcacaggaa ggggtcgcca tgctgaccga ggaattgaaa cgttacgagc ctgacctcgt
    12901 cgccatccgc catgacattc atcgccatcc cgagacaggt ttcgaggagc atcgcacagc
    12961 caagctcgtc gccgcgcgct tgcgcgaatg gggcgtcgac gtcgccgaag gcctcgccaa
    13021 gaccggaatc gtcggcacgc tgcgcggcgc ccggcctggg cagaaggcga tcgggcttag
    13081 ggccgatctc gatgcgctcc acatcagcga aaagacgggc cgcgattacg cctctgaaat
    13141 ttccggcaag atgcatgcct gcggtcacga cggccatacg gcgatgctgc ttggcgcggc
    13201 gcgttatctc gcggcgcatc gcgactttgc cggcgtcgtc catttcatct tccaaccggc
    13261 ggaggagggg ctcggcggcg gccgcctgat ggtcgaagag ggtctgttcg agcgcttccc
    13321 ggtcgatgcg gtctatggga tgcataacag cccgggcctt ccggtcggaa aattcgcgac
    13381 gcgggtcggg ccgttcctgt cggcgagcga cacttggacc gtgacctttc gcggcacggg
    13441 cggccatggc ggcgccggcg cccaccgcgc caaggatccg accatcccgc aggcgcattt
    13501 cgtacttgcg ctgcagacaa tcgtcggccg cagcgtgccg gcggtcgaga cggcggtggt
    13561 cagcgtcggc tcgatcgcgg gcggcgatca cgcctcgccg aacatcatcc cggcggagat
    13621 cgtggtacgc ggcaccgcgc ggtcttataa gccggctatc cgcgatttga tcgagaagcg
    13681 cctcggcgag ctcgcccatg cgctcgccgc cgcctatggc tgcgtcgccg aagtggagta
    13741 cgagcgccgc tatccgccgc tgatcaccca cgccgaggag acggcgaca
//
LOCUS       DATGJW010000067         4044 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1360623, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000067 DATGJW010000000
VERSION     DATGJW010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4044)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4044)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4044
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1360623"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..970)
                     /locus_tag="VKU03_02005"
     CDS             complement(<1..970)
                     /locus_tag="VKU03_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLJ70061.1"
                     /translation="MDSSLIDSKAPAVSPPDDLESGPKIVNFRLANGLEVVVIPDHRA
                     PVVTHMVWYRNGSADDPPGKSGIAHFLEHLMFKGTAAHPQGEFSKVIAELGGQENAFT
                     GNDYTAYFQRVAKQHLGVMMAFEADRMTGLQLTDEVVAPERDVVLEERRMHCDADPGA
                     QLGEAMQAALFTHHPYGTPIIGWAHEIEDLSRADALAYYRRFYTPQNATLIVAGDVEP
                     DEVRTLAETTYGKIKPLGETPQRRRPKEPPALARRTVTVNDEKVEQPVWQRCYLTPSY
                     RTAQPGEAEAIEVLAHILGGGQTSLLYRVLTIEERIAAAIGAQYLGS"
     gene            complement(1104..1487)
                     /locus_tag="VKU03_02010"
     CDS             complement(1104..1487)
                     /locus_tag="VKU03_02010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890604.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ70062.1"
                     /translation="MAINPQFIKAPDGSELVVITRAEFDALVNRFDEDEEEAAIFDAR
                     IAELRANEAETLPQEITQLMLNGDSLLKALRKWRDQTQLCLESKTGLSQSYISDLESG
                     KKQGTPETLRKIAAALQIEPKWLGV"
     gene            complement(1558..2985)
                     /locus_tag="VKU03_02015"
     CDS             complement(1558..2985)
                     /locus_tag="VKU03_02015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015205 - nucleobase transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleobase:cation symporter-2 family protein"
                     /protein_id="HLJ70063.1"
                     /translation="MSDVAIKPVDEILPAPRLTALGLQHVLVMYAGAIAVPLIVAGAL
                     GLPAEQRAILINADILACGLASIVQSIGFPGVGIRLPVMMGVTFASVAPMLAMITAAK
                     EAGGTNFSAVAVLTTIYGAVAAAGIFAIVVAPYMSRLLRLFPPVVTGSIILVIGLSLM
                     RIGVGWVEGPFVVPNIVDGVFKGVIPNPNRDAIAGFVLSLIVLLVILGVTKFGKGFWA
                     NIAVLIGIVVGAIIAAIIGKMEFGAVAKASWLGVTVPFQFGFPSFQLVPILTMCVVMV
                     VVMIESTGMFLALGEMTGKTIGPAELTAGLRADGVGTIIGALFNAFAYTSFSQNVGLV
                     GVTGVRSRFVTAVGGVILIVLSLIPKLAAVVGAIPVEVLGGAGLVMFGMVAATGVRIL
                     SAVDFKNNRNNLFIVAIAVAFGLLTLISPTFFKNFPTALKPILDSGIILTTVVAVALN
                     AYYNGLGSQGEAESRLTEAARAAEH"
     gene            complement(3135..3401)
                     /gene="rpsT"
                     /locus_tag="VKU03_02020"
     CDS             complement(3135..3401)
                     /gene="rpsT"
                     /locus_tag="VKU03_02020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172624.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HLJ70064.1"
                     /translation="MANTSSAKKAVRKIARRTAVNKSRRSEMRTYVRKVEEALAAKDA
                     AAAAAALNEAQPRLARAAQKGIVHKNAASRKISRLSKRVKALAG"
     gene            complement(3533..>4044)
                     /locus_tag="VKU03_02025"
     CDS             complement(3533..>4044)
                     /locus_tag="VKU03_02025"
                     /inference="COORDINATES: protein motif:HMM:NF014035.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HLJ70065.1"
                     /translation="GAVDAALPLVLIAVALYFAFSPTMSDADRAKRLGERAYAFSVAP
                     LVGFYDGVFGPGAGSFYIIGFVALVGYGVTRAVANARLANFASNLGSLAIFALGGHIV
                     LIAGLATGAGQFLGSRLGAHAVLGSGARLVRPLIVAVSCAMAVKLAISPGHPAHSWLL
                     HAAKMLGGG"
BASE COUNT          772 a         1275 c         1331 g          666 t
ORIGIN      
        1 ccgagccgag atattgagcg ccgatcgctg cggcgatccg ctcttcgatg gtcaggacgc
       61 gatagagcag gctggtctgg ccgccgccga gaatatgggc gagcacctcg atcgcctccg
      121 cttcgccggg ctgggcggtg cggtacgagg gcgtcaaata gcagcgctgc cagaccggct
      181 gttcgacctt ctcgtcgttc accgtcaccg tccgccgcgc cagcgccggc ggctctttcg
      241 gacgccggcg ctgcggcgtc tcgccgagcg gcttgatctt gccgtaggtc gtctcggcga
      301 gcgtgcgcac ctcgtccggc tcgacatcgc cggcgacgat cagcgtcgca ttttgcggcg
      361 tatagaagcg ccggtaatag gcgagcgcgt cggcgcggct taaatcctcg atttcatgcg
      421 cccagccgat gatcggcgtc ccatagggat gatgggtgaa caaggccgcc tgcatcgctt
      481 cgccgagctg agcgccggga tcggcgtcgc aatgcatgcg gcgctcctcc agcaccacgt
      541 cgcgctccgg cgccacgacc tcgtcggtga gctggagtcc ggtcatgcgg tcggcctcga
      601 acgccatcat cacgccgaga tgctgcttgg cgacgcgctg gaaataagcg gtgtagtcgt
      661 tgccggtgaa ggcgttctcc tggccgccaa gctcggcgat gactttggag aattcgccct
      721 gcggatgggc ggcggtgcct ttgaacatca aatgttcgag aaaatgggcg atgccggatt
      781 tgccgggcgg atcgtcggcg gagccgttgc gataccacac catatgggtg acgacggggg
      841 cgcgatggtc gggaatgacg acgacctcca acccgttggc gaggcggaaa ttgacgatct
      901 tgggaccgct ttccaggtcg tcgggcggcg aaacggcggg agctttcgag tcgatcaaac
      961 ttgagtccat gggcgccgcg acaattggag ttcacacgag atcgaacggg gaaaacgcct
     1021 cgccgacatg aaatcgacat ataggcggcc ctggagcaaa gcgccagcga ttcgggacgg
     1081 ggccgactaa tcggcggggg cgttcaaacg cccagccatt tcggctcgat ttgcaaggcg
     1141 gcggcgatct tgcggagcgt ttccggcgtt ccttgctttt tgccgctttc aagatcgctg
     1201 atgtagcttt gcgaaaggcc ggtcttagac tccagacaaa gctgagtctg atcgcgccat
     1261 ttccgcaacg ccttgagcaa cgaatcgccg ttcagcatga gctgcgtgat ttcttgcggc
     1321 aacgtttcgg cttcgttcgc gcgaagctcg gcgatacgcg cgtcgaaaat cgcggcctcc
     1381 tcttcgtcct cgtcgaaacg attcacaagc gcatcaaatt cggcgcgggt aatgacgacg
     1441 agctccgacc cgtccggcgc cttaatgaat tgcggattga tggccatgcg tcttccccgc
     1501 atacgtcctg ccggtcggga agcggcggcg cgtttttttt atcgcgccgc gacgccatca
     1561 atgctcggcc gcccgcgcag cctcggtcaa tcgactctcc gcctcgccct gcgagccgag
     1621 cccattgtaa taggcgttga gcgcgaccgc gacgacagtc gtcaggatga tgccggaatc
     1681 gaggatcggc ttcagcgccg tcgggaagtt cttgaagaag gtcggcgaaa tcagcgtcag
     1741 caggccgaag gcgacggcga tggcgacgat gaacagattg ttgcggttgt tcttgaagtc
     1801 gaccgcgctc aaaatcctca cgccggtcgc cgcaaccatg ccgaacatga cgaggccggc
     1861 gccgccgagc acttcgacgg ggatcgcgcc gaccaccgcc gccagtttcg gaatgaggct
     1921 gagcacgatc aggatcacgc cgcccaccgc ggtgacgaac cgcgagcgca cgccggtcac
     1981 cccgaccagg ccgacattct gcgagaagga cgtgtaggcg aaggcgttga acaacgcgcc
     2041 gatgatcgtg ccgacgccgt cggcgcgaag gccggcggtg agctcggccg ggccgatcgt
     2101 ctttccggtc atttcgccga gcgccaggaa catgccggtc gattcgatca tcacgacgac
     2161 catcacgacg cacatcgtca ggatcggcac gagctggaag ctcggaaagc cgaattggaa
     2221 cggaacggtg acgccgagcc aagaggcctt ggcgaccgcg ccgaactcca tcttcccgat
     2281 aattgcggcg atgatcgccc cgacaacgat gccgatcaaa acggcgatat tggcccagaa
     2341 gcctttgccg aacttggtga cgccgaggat caccaacaac acgatcagcg acaggacgaa
     2401 tcccgcgatc gcgtcgcggt tgggattggg gatcacgccc ttgaagacgc cgtcgacgat
     2461 gttcggcacg acgaaggggc cttcgaccca gccgacgccg atgcgcatca gcgacaggcc
     2521 gatcaccaga atgatcgagc cggtcacgac cggcggaaac agccgcagca agcggctcat
     2581 atacggcgcg acgacgatgg cgaagattcc ggcggcggcc accgcgccgt agatcgtcgt
     2641 cagcacggcg acggccgaga aattcgtccc gcccgcttct ttcgccgcgg tgatcatcgc
     2701 cagcatcggc gcgaccgagg cgaaggtgac gcccatcatc accggcaggc ggatgccgac
     2761 gccgggaaag ccgatcgatt ggacgatgct ggcgaggccg caagcaagaa tgtcggcgtt
     2821 gatcaggatg gcgcgctgct cggccgggag gcccaaagcg ccggcgacga tcagcggcac
     2881 cgcgatcgcg ccggcataca tcaccaagac atgctgcaag cccagcgcgg tcaggcgcgg
     2941 cgcgggcaat atttcatcga ctggcttgat cgcgacgtcg gacatggctg ctccccccga
     3001 agatttgccg ggaggatcat agacgccgct caaaccggct gtatcgtgaa actttcataa
     3061 attcctgccg cggagctgag ataataacag ctctgtccat gcggcggcgc atgggcggcg
     3121 cgaggaagcg cggatcagcc ggcgagggcc ttgacccgct tgctcagccg ggaaatcttc
     3181 cgcgacgcgg cgttcttgtg gacgatgccc ttctgggcgg cgcgagcgag ccggggctgc
     3241 gcttcgttca gggccgcggc cgccgcggcg gcgtccttgg cggcgagcgc ctcctccacc
     3301 ttgcgcacat aggtgcgcat ttcgctgcgg cgcgatttgt tcaccgccgt gcgccgggcg
     3361 atcttgcgga cggctttctt cgccgaactc gtattggcca tctggacgag aatccttgga
     3421 aattttctaa aggctcaaga ctcgccggcg agcctcggcc caaaaacaaa acgaccgggc
     3481 gtccaaggcc cagcctgtcg gccgcgctta tagacaggcg ctttcgtcgc cgtcaaccgc
     3541 cgccaagcat tttcgcggcg tgaagcagcc aggaatgcgc cggatgaccc gggcttatcg
     3601 cgagcttgac ggccatggcg caggagacgg cgacgatcag cggccggacc agccgcgcgc
     3661 ccgagccgag caccgcatgc gcgccgagtc gcgagccgag aaattgccca gcgcccgtcg
     3721 ccaggccggc gatcaggacg atgtgcccgc caagcgcgaa aatggcgagg cttcccaagt
     3781 tgctggcgaa attggcgagg cgcgcgttgg cgacggcgcg cgtcacgcca tagccgacca
     3841 gcgcgacaaa gccgataatg tagaacgagc cggcgccggg gccgaacacg ccgtcataga
     3901 agccgaccag cggggcgacc gagaaggcgt aggcgcgctc gccgaggcgc ttcgcgcggt
     3961 cggcgtcgct cattgtgggc gaaaaggcga aatagagcgc gacggcgatc agcaccaagg
     4021 gcagggccgc atcgaccgcg ccgc
//
LOCUS       DATGJW010000068         2955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_634650, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000068 DATGJW010000000
VERSION     DATGJW010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2955
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634650"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..658)
                     /locus_tag="VKU03_02030"
     CDS             complement(<1..658)
                     /locus_tag="VKU03_02030"
                     /inference="COORDINATES: protein motif:HMM:NF024956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0030420 - establishment of competence for
                     transformation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HLJ70066.1"
                     /translation="MKAEGRAGVWAGAGPVAAATRPALHALYASAFAREVEERRLSLW
                     VAVAAIAGVALYFTADREPILWLSGLIAGAFCGFALATRGRPIAFAAMVGLAALSGGF
                     FSAGWRTARVATPMLDHIRVVKLKGYIEELDLRRVGARLILRVEDAGDMPAAIAPRRV
                     RVTMRKAPDAAAGDYVALTARLLPPTHAVLPGGYDFARDAFFAGVGAVGSTFGAVVKI
                     A"
     gene            784..2205
                     /gene="gltX"
                     /locus_tag="VKU03_02035"
     CDS             784..2205
                     /gene="gltX"
                     /locus_tag="VKU03_02035"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLJ70067.1"
                     /translation="MTKVVTRFAPSPTGFLHIGGARTALFNWLYARRQNGTFLLRIED
                     TDRERSTDAAVTAIIDGLAWLGLKWDGEAISQFARVARHREVAEMLLASGGAYRCYAS
                     PEELEEIRQKARAEGKPMRYDGRWRDRDPSEAPPGVKPVIRLRAQQEGETVIDDKVQG
                     RVVFANKDLDDLVLLRSDGTPTYMLAVVVDDHDMGVTHIIRGDDHLTNAARQLQIYQG
                     LGWEAPVMAHIPLIHGPDGAKLSKRHGALGVDAYRAMGYLPAALRNYLLRLGWSQGDK
                     EFFSTEEMIEAFDLSSIGRSPSRFDFAKLENMNGHYMRATPDDELLAILIGSLPHLPG
                     GPELAQHLDEAGRAKLAAALPGLKERAKTLVELIDGAGYLFAERPLRLDDRAKEIVAQ
                     GGRAHISALLPRLAAVGDWTPPSIEAAVRDYAGESGAKLGQIAQPLRAALTGRATSPG
                     IFDVLAVLGREESLGRLEDCAAL"
     gene            2357..>2955
                     /locus_tag="VKU03_02040"
     CDS             2357..>2955
                     /locus_tag="VKU03_02040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLJ70068.1"
                     /translation="MTRLETIASVGRRALPNHWDVLALGAIMAAFVGIVHAFHGMNVP
                     LPAANQPAVDLSYWNLPYYALRTILRMFAALAASFVFTFTYATLAAKSRRLEIVLIPI
                     LDVLQSVPILGFLSFTVTFFLGLFPGNELGAECAAIFAVFTSQAWNMAFSFYQSLRTV
                     PRDLDEVSRGFRLTGWQKFWQLEAPFAVPGLIWNTMMSMS"
BASE COUNT          484 a         1034 c          944 g          493 t
ORIGIN      
        1 gcgcgatctt gaccaccgcg ccaaaggtcg atccgactgc gccgacgccc gcgaaaaacg
       61 cgtcgcgcgc gaaatcgtag ccgcccggca agacggcatg ggtcggcggc agaagccgcg
      121 cggtcagcgc gacatagtcg ccggccgccg catctggcgc cttccgcatc gtcacccgga
      181 cgcggcgcgg cgcgatcgca gccggcatgt cgccggcgtc ctcgacgcgc aggatcagcc
      241 gcgcgccgac gcggcgcaag tcgagctcct cgatatagcc cttcagcttg acgacgcgga
      301 tatggtcgag catcggcgtc gcgacgcgcg cggtgcgcca accggccgaa aagaaacctc
      361 cactcagcgc ggcgagcccg accatcgccg caaaggcgat cgggcggccg cgcgtcgcca
      421 aggcgaagcc gcagaatgcg ccggcgataa gccccgacag ccacagaatc ggctcgcggt
      481 cggcggtgaa gtaaagcgcg acgcccgcga ttgcggccac ggcgacccag agcgacagcc
      541 ggcgctcttc gacctcgcgc gcgaaggcgc tcgcatagag cgcgtggagc gccggccgcg
      601 tcgccgccgc gaccggcccc gcgcccgccc atacgcctgc tcgcccctcc gccttcattg
      661 agctttaagc cctttggcgc cgtcagcccg ccatgataca ggggccgcca atgcaggcga
      721 ggccgaaaat tctgattttt ccctttttcg gccgcccgac ccggtgctgg attggctaga
      781 acaatgacga aagtggtgac gcgctttgcg ccctctccga cgggctttct gcatattggc
      841 ggcgcgcgca ccgccttgtt caactggctt tacgcgcgcc gtcagaacgg aacctttctg
      901 ctgcgcatcg aggatacgga ccgcgagcgc tcgaccgacg ccgccgtgac ggcgatcatc
      961 gacggcctcg cttggctcgg cttaaagtgg gacggcgagg cgatcagcca attcgcgcgc
     1021 gtcgcgcgcc atcgcgaagt cgccgagatg ctgctcgcct cgggcggcgc ctatcgctgc
     1081 tacgccagcc cggaagagct cgaagagata cgccagaagg ccagggccga aggcaagccg
     1141 atgcgctatg acgggcgctg gcgcgaccgc gacccgtcgg aggcgccgcc cggcgtcaag
     1201 ccggtgatcc gcctgcgcgc ccagcaggag ggcgagaccg tcatcgacga taaggtacaa
     1261 ggccgcgtcg tcttcgccaa taaggacctc gacgatctcg tccttctccg ctcggacggc
     1321 acgccgacct atatgctcgc ggtggtggtc gacgatcacg atatgggcgt gacccacatc
     1381 attcgcggcg acgatcacct caccaacgcc gcgcgccagt tgcaaatcta tcagggactc
     1441 ggctgggagg cgccggtcat ggcgcatatc ccgctgatcc atgggccgga cggggccaag
     1501 ctctccaagc gccacggcgc gctcggcgtc gacgcgtatc gcgccatggg ctatctgccc
     1561 gcggctttgc gcaactatct cttgcggctc ggctggagcc agggcgacaa ggaattcttc
     1621 tcaaccgagg agatgatcga agccttcgat ctttcgtcga tcggccgctc gccgtcgcgg
     1681 ttcgatttcg ccaagctcga aaacatgaac ggccactata tgcgcgcgac gccggatgac
     1741 gagctcctcg cgatcctgat cggcagcctg ccgcatctgc cgggcggtcc cgaactcgcc
     1801 cagcatctcg acgaggccgg gcgcgccaaa ttggccgcgg ctttgccggg cctcaaagag
     1861 cgcgccaaga ccctggtcga actcatcgac ggcgccggct atttgttcgc cgagcggccg
     1921 ctgcggctcg acgaccgcgc caaggaaatc gtcgcgcaag gcggacgagc gcatatctcg
     1981 gcgctgctgc cgcggctggc ggcggtcggc gactggacgc cgccttcgat cgaagcggcg
     2041 gtgcgcgact atgccggcga gagcggcgcc aagctcggcc aaatcgcgca gcccttgcgc
     2101 gccgccctga ccggccgggc gacatcgccg ggcattttcg acgtgctcgc agtgctcggc
     2161 cgcgaggaga gtctcggccg gctggaggat tgcgcggcgc tgtagccgcg gccgggaccg
     2221 cggcgacaag acctgtcgca tcgctgtcac atctctcggc cagagaggcg gaccgaggcg
     2281 ccggcatttt cctgttgcct tttctgccga atttatggtt ccccagggtc gaatcgcccc
     2341 tcagcggaat tggccgatga cgagactgga gaccatcgca tcggtaggcc gccgcgcctt
     2401 gcccaatcat tgggacgtgc tcgcgctcgg cgcgatcatg gcggcctttg tcggaatcgt
     2461 ccacgccttt cacggcatga acgttccgct gcccgccgcc aaccagccgg cggtggactt
     2521 gagctattgg aacctgccct actatgcgct gcgtacgatc ctgcgcatgt tcgcggcgct
     2581 cgccgcgtcc ttcgtcttca ccttcaccta cgccacgctc gccgccaaga gccggcgcct
     2641 cgaaatcgtc ctgatcccga ttctggacgt gctgcaatcg gtgccgatcc tcggctttct
     2701 ctcgttcacg gtgacgttct ttctcggcct ttttcccggc aacgagctcg gcgcggaatg
     2761 cgcggcgatt ttcgcggtct tcaccagcca ggcctggaac atggccttct ccttctatca
     2821 atcgctgcgc accgtgccgc gcgatctcga cgaggtctcg cgcggcttcc gcctcaccgg
     2881 ctggcagaaa ttctggcaat tggaggcgcc gttcgccgtg ccgggcctga tctggaacac
     2941 gatgatgtcg atgtc
//
LOCUS       DATGJW010000069        10771 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_634795, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000069 DATGJW010000000
VERSION     DATGJW010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10771)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10771)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10771
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_634795"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..493)
                     /locus_tag="VKU03_02045"
     CDS             complement(<1..493)
                     /locus_tag="VKU03_02045"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: protein motif:HMM:NF012690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HLJ70069.1"
                     /translation="MRIESDIKLDFKDVLIRPKRSTLNSRNDVDVHRSFRFLHTGGEW
                     TGFPLIAANMDVTGTIAMARALGRFGAMTALHKHYPEKTLAEFFASGDGAHAFYSIGT
                     TEADFAKLAKVKRSAAIKHLCLDVANGYSEKFLDTVKRARDDNPDAVIMAGNVVTGDM
                     TEAL"
     gene            692..1438
                     /locus_tag="VKU03_02050"
     CDS             692..1438
                     /locus_tag="VKU03_02050"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLJ70070.1"
                     /translation="MRLKGKSAVVTGGAQGFGLGIAETFAREGANVAVLDYNGEAAKA
                     AAAKLGGSAIALTCDVSKKDQVDAAAKAAIGAFGRIDIIVNNAGTTYRNRPMLEVDEA
                     EFDRVMAVNVKSIFLMAHAFVPHMREKGGGAIINIGSTAGLRPRPGLTWYNTTKGAVH
                     LMTKSMAVELAPDKIRVCALAPVAGETPLLSSFLGEDTPERRAAFRATVPLGRFSTPQ
                     DIANSALFLASDEADFLTGVVLEVDGGRCI"
     gene            complement(1615..2118)
                     /locus_tag="VKU03_02055"
     CDS             complement(1615..2118)
                     /locus_tag="VKU03_02055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HLJ70071.1"
                     /translation="MSEPTPLENLPPSPRKVYDAARALGLDIEVHIRTESTRTAEEAA
                     AACGCSVAQIVKSLVFRGGASGKTLLFLVAGDNRLDEALAGALVDDGLERPDGRSVRE
                     TTGFAIGGIPPFGHAVPLETIMDEDLLAHDVVWAAAGAPNAVFPIAPHKLRAATRAQV
                     ARLAAAL"
     gene            complement(2210..3325)
                     /locus_tag="VKU03_02060"
     CDS             complement(2210..3325)
                     /locus_tag="VKU03_02060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ70072.1"
                     /translation="MTMSYYTLGRSGLRVSRLALGTMTFGTDWGWGADKETARSLFDR
                     YVEQGGNFFDTADLYTNGASETWLGEFVADRRLRDKAVIATKFSFNAEPGNPNAGGNG
                     RKNMLRAVEGSLKRLKTDYIDLYILHAWDRVTPAEEVLRTFDDLVRAGKVRHIGLSDV
                     PAWYASRMQAIAELRGYEPLSALQLEYSLAERNIENEFVPLATAQGMGVMVWSPLASG
                     LLSGKYRPSQDGGGAGAGRLETLKGASNPGFQKFTPRNWAIVAELEAVAKETGRGMAQ
                     VALNWVANRPGVASVILGATKLAQLEDNLQALTFALPPELAARLDTASAPERSFPYTF
                     FDAEMQGMIHGGVAVGAKPVTYQPPIEISGAGADVTT"
     gene            3552..4385
                     /locus_tag="VKU03_02065"
     CDS             3552..4385
                     /locus_tag="VKU03_02065"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLJ70073.1"
                     /translation="MRKASRRAALATAGLFALGSSLVSNVGAASAAQHIVVIPGVGPG
                     DATQPGPLPPVPPAPPVKGAYGGGFIEFLITGDSGGQSVSRPAASSYATPVYGPPPAP
                     AYSNEAVAALPEPAPANEPDLAVRRESPAPEFRRQEVAYDGDQKPGTIIIDTPRKFLF
                     LVEPGGRAIRYGIGVGRPGFEWSGVKRVTRKAEWPGWTPPPQMLLRRPDLPSHMDGGL
                     GNPLGARALYLGSSLYRIHGTNEPWTIGQNVSSGCIRMMNQDVIDLYNRVPIGAKVVV
                     L"
     gene            complement(4492..5238)
                     /locus_tag="VKU03_02070"
     CDS             complement(4492..5238)
                     /locus_tag="VKU03_02070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2076 domain-containing protein"
                     /protein_id="HLJ70074.1"
                     /translation="MTPQERQLLGGLFQRIASTASNSRDPEAENFISEAVRMQPHAPY
                     VLAQTVLVQQQALEAAAKRVQELEAQIQGQNPPEQQEGSFLGNLGRSLFGGGQSAPPA
                     PRSNYDASAYTKGSSAPPPGPQQAYPQQNYGPPPGAGPWGAPPSSGGGFLHNALTTAA
                     GVAGGMMAADALRGMFGGHAYGLGGGYGLGGVGGETVVNNYYENADPAGQQAQDTLQD
                     MDQDQDDLQDAADDSGFDDGGNDNDGSFDT"
     gene            complement(5348..6025)
                     /locus_tag="VKU03_02075"
     CDS             complement(5348..6025)
                     /locus_tag="VKU03_02075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HLJ70075.1"
                     /translation="MADLITGPRLEPKSGQAKQLVVFLHGYGADGNDLIEIGRHWRAW
                     LPDAAFVSPHAPEPCAMSPMGRQWFALTMRDPDERWRGVVKARPVLDAFLDAELARLD
                     LDESRLALVGFSQGTMMALHVGLRRPRAPAAILGYSGVLVGPDRLAEAAARDVRGAPP
                     PILLVHGDQDQLIPFEALFESAEALAKANIPCQWHLSLGVAHGIDEGGLRHGGLFLAK
                     SFGVKAG"
     gene            complement(6057..6686)
                     /locus_tag="VKU03_02080"
     CDS             complement(6057..6686)
                     /locus_tag="VKU03_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase 2 family
                     protein"
                     /protein_id="HLJ70076.1"
                     /translation="MNVAIDQLMARDPDLIGKLLAAGGRPPLRRREPGFAGLVWIIVS
                     QQVSTASANAIFKRVEANLAPLSAAGVLAADDEALRACGLSWPKMRALRALAGAVEDR
                     TLDLDRLAPLAAEEAHRALVAVKGIGPWTADIFLLFCLGHPDAFPAGDLALQEAARLA
                     LGLKKRPDAKALHKIAERWRPLRGVAARLLWAYYRAARQGREGMALAGA"
     gene            6881..7771
                     /gene="gluQRS"
                     /locus_tag="VKU03_02085"
     CDS             6881..7771
                     /gene="gluQRS"
                     /locus_tag="VKU03_02085"
                     /EC_number="6.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA glutamyl-Q(34) synthetase GluQRS"
                     /protein_id="HLJ70077.1"
                     /translation="MPNDQQRPVYRFAPTPNGRLHLGHAYSALMNAKGAERFGGRLLL
                     RMEDLDRSRCKPEYEQAAIADLAWLGLSFDPAIRRQSEHVSDYAAAYRVLEALGLLYP
                     CFCTKEEIAKASRAAGLGRDPDGAPLYAGACRRRGSPKRHGLAPTLRLDMARALALAG
                     SELFWREFGEGDDERRERADPAAWGDVALKRRGAPATYHVAVVVDDALQGVSDVVRGR
                     DLFAATGLHRLLQVLLNLPPPRYRHHRLVLDAEGEKMSKSASSTPLCRLREAGVSAAQ
                     IRAALGFGPAQGRRIEAWIS"
     gene            complement(7764..9785)
                     /locus_tag="VKU03_02090"
     CDS             complement(7764..9785)
                     /locus_tag="VKU03_02090"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HLJ70078.1"
                     /translation="MAATIALAQRRAGPSRAVVDLGREQPIEAGERRPDHHERRNISF
                     RWLSGAVLTGFCGIALVGSALYLDLDRQSNFAQAPEIAAPARPTSEAEQGVNPGKGDR
                     LVRPVDIVAAKQTFRAATTIKVGDKEVVKPRTFTFVSTTLTTTPTGFAGEVPEFNPLK
                     LTAASIEEPDQPTDPGQVQDDADVTFTTRDLTAADAALISDRLSAAEAQAEVAESLRA
                     LTAAKNAPPIPPQLLLTRTSRAGLDPVGGLAYAAAGNVGANAPFASIDVRMVPENVTN
                     VPRSPASETAEPTERLVVLRHGETLADLLAANGATKPDAVKIVAAFGAKKGDGLIAEG
                     QKALLTFAETDAPGPRKIARVSLYADEQLKATVAANDAGDYVAVTLPPPAAKKSPEGP
                     GGMSLYESFYQTVLKQGVPKPIIEEMVKAFANDVDFQRSVTAGDSVEAFYSEPDDIDS
                     RPELLFGMFTAHDQTFKYYRFQTPDDGRVDYYDENGRSVRKFLIRKPIAAGEMTSPFG
                     MRYHPILHYSRMHTGVDWANAPGTPVLAAGNGVIIKAGGEQGYGRRIEIQHANGYVTT
                     YSHLSGFARGIVEGAHVNQGQVIGYLGQSGMATGPHLHYEVIVNGRFVDPMAIKLART
                     REFDGKMLAAFKRERDRIDQLIGEAPNAAPVAQAPSAGPGVTPLAQKTN"
     gene            9839..10288
                     /locus_tag="VKU03_02095"
     CDS             9839..10288
                     /locus_tag="VKU03_02095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70079.1"
                     /translation="MIIRRELSSAGGSAIDGRVEVAINNGANGAIGWPAESPTEASFP
                     HSYRVPPNRMNFFQKPFDSLKTQSYIRATDGDDALGGRKAIRFSRVYQAHASRPKGWG
                     EDGSVFLEAQAFGRVFCCLTIESEERETWTAEVLADRPLTRAVEETL"
     gene            10393..>10771
                     /locus_tag="VKU03_02100"
     rRNA            10393..>10771
                     /locus_tag="VKU03_02100"
                     /product="16S ribosomal RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00177"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00177"
BASE COUNT         1762 a         3406 c         3688 g         1915 t
ORIGIN      
        1 ccagcgcctc cgtcatgtcg ccggtgacga cgttgccggc catgatgacc gcgtcgggat
       61 tgtcgtcgcg ggcgcgcttc accgtatcga ggaatttctc ggaatagcca ttggcgacgt
      121 cgaggcagag atgcttgatc gccgcgctcc gcttcacctt ggcgagcttg gcgaaatcgg
      181 cctcggtcgt gccgatcgaa tagaaggcgt gcgcgccgtc gccgctggcg aagaactcgg
      241 ccagcgtctt ttccggatag tgcttatgca gcgccgtcat cgcgccgaaa cgcccgagcg
      301 cgcgggccat ggcgatggtg ccggtcacgt ccatattggc ggcgatcagc ggaaagccgg
      361 tccattcgcc gccggtatgc aggaagcgga agctgcgatg gacgtcgaca tcgttgcggg
      421 agttcagggt cgagcgcttc ggacggatta gcacgtcctt gaagtcgagc ttgatatcgc
      481 tttcgatacg catagggttt tccaaaagaa ggcttgaggc gggcaaacgg cctgatatct
      541 gtctgatgct taccatgttc aagggcccgc ggcccatcct tccgaaggat ggccgcgggc
      601 ccttttttgt cgccagggcg cgaccgggct gataatcgaa aatcccagga gccgcccgtt
      661 agcggcccat cgaatcgggt gaggagcgag catgcgattg aagggaaaga gcgcggtcgt
      721 gaccggcggc gcacagggtt tcggcctcgg catcgccgaa acatttgcgc gggaaggcgc
      781 gaatgtcgcg gtgctcgatt ataacggcga ggcggcgaag gcggcggcgg ccaagctcgg
      841 cggctcggcg atcgctctta cttgcgacgt ctcgaagaag gaccaagttg acgccgccgc
      901 caaggcggcg atcggcgcgt tcggccgcat cgacatcatc gtcaacaatg cgggcaccac
      961 ctaccgcaat cggccgatgc tcgaagtcga cgaggcggag ttcgaccgcg tcatggcggt
     1021 caatgtgaag tcgatcttcc tgatggcgca tgctttcgtg ccgcatatgc gcgagaaggg
     1081 cggcggcgcg atcatcaata tcggctcgac cgcgggcctc aggccgcggc cggggctgac
     1141 ctggtacaac acgaccaagg gcgcggttca tctgatgacc aagtcgatgg ccgtggagct
     1201 cgcgccggac aagatcaggg tctgcgccct ggccccggtg gccggcgaga cgccgctcct
     1261 gtcgagcttt ctcggcgagg acacgcccga gcgccgcgcc gccttccgcg ccaccgtgcc
     1321 gctcggccgc ttctcgacgc cgcaggacat cgccaattcg gccctgttcc tcgcttcgga
     1381 cgaagcggac tttctcaccg gcgttgtgct ggaggtcgat ggcgggcgct gcatttgagg
     1441 cgaatcctct cccgtgaagc gaagcggaac gggggagggg gaccatgcga agcatgatgg
     1501 agggggccgc ggtggttggg aattatcctg agagaccgag gccccctcca ccgcgctggc
     1561 gcgcggtccc ctcccccgtc tttggcgggt gaggaaatcg gtcgccgacc gccgctacag
     1621 cgctgccgcg agccgcgcga cctgcgcccg ggtcgcggcg cgcagcttgt gcggcgcgat
     1681 cggaaacacc gcgttcggcg cgccggccgc ggcccagacg acatcgtggg cgagcaggtc
     1741 ctcgtccatg atcgtctcca gcggaacggc atggccaaag ggcggaatgc cgccgatggc
     1801 gaagccggtc gtctcgcgca cggaacgacc gtccggccgc tcgagaccgt cgtcgaccaa
     1861 ggcgccagcg agcgcttcgt cgagccgatt gtcgccggcg acaaggaaca ggagcgtctt
     1921 gccgcttgcc ccgccgcgaa agacgaggga tttcacgatc tgcgccacgc tgcagccgca
     1981 agccgctgcg gcctcttcgg ccgtacgcgt cgattcggtc ctgatatgga cctcgatgtc
     2041 gagcccgagc gctcgcgccg cgtcgtagac tttgcgcggg cttggcggca ggttttccag
     2101 gggcgttggc tcactcattt ttgcactcac gatagaggct gtttcggcgc gaaggcgttt
     2161 atgcgccgcg ccgaggcgat acgccatgca tgccggctat accggcaagt caggtcgtga
     2221 cgtcggcgcc ggcgccggaa atttcgatcg gcggctgata ggtgacgggc ttggcgccga
     2281 cggcgacgcc gccatggatc atgccttgca tctccgcgtc gaagaacgtg taggggaagg
     2341 agcgctccgg cgcgctcgcc gtatcgagcc gcgcagcgag ttccggcggc agcgcgaatg
     2401 tcagggcttg cagattgtct tcgagctgcg cgagcttggt cgcgccgaga atgactgaag
     2461 cgacgcccgg ccggttggcg acccagttga gcgcgacctg cgccatgccg cggccggtct
     2521 ctttggcgac cgcctccaat tcggcgacga tcgcccagtt gcgcggcgtg aacttctgaa
     2581 agccgggatt gctcgcgccc ttgagcgtct ccagccggcc cgcgccggcg ccgccgccgt
     2641 cctggctcgg gcggtatttg ccgctgagca agccgctggc gagcgggctc caaaccatca
     2701 cgcccatgcc ttgggcggtc gcgagcggga cgaactcgtt ctcgatgttt cgctcggcga
     2761 gcgaatattc gagctgcagc gccgaaagcg gctcatagcc gcgcagttcg gcgatcgcct
     2821 gcatgcgcga agcgtaccaa gccggaacgt cgctcaagcc gatatgccgc accttgccgg
     2881 cgcggaccag atcgtcgaac gtgcgcagga cctcttccgc cggcgtgact ctgtcccagg
     2941 cgtgcaggat gtagagatcg atgtagtcgg tcttgagccg cttcagcgag ccctcgacgg
     3001 cgcgcagcat gtttttgcgg ccattgccgc cggcattggg attgcccggc tcggcgttga
     3061 aagagaattt ggtcgcgatc accgccttgt cgcggagccg ccggtcggcc acgaactcgc
     3121 cgagccaggt ctcgctggcg ccgttggtgt agagatcggc ggtgtcgaag aaattgccgc
     3181 cctgctcgac atagcgatcg aacaggctgc gcgccgtctc cttgtccgcg ccccagcccc
     3241 aatccgtgcc gaaggtcatg gtcccgagcg ccagccggct gacgcgcaat cccgagcggc
     3301 cgagcgtgta ataagacata gtcaatggcg ttctccttgt ttcgcaatca cggcggggcg
     3361 cttggaggga aagctccgag agacaaaacg agagggcttg cgaagaaagc acacgcgccg
     3421 atcgtacccg cgcgcgtcac gtcttgataa ccatctcgcc ggatagtccg tattttcacg
     3481 acaggtttcg cgtatgatta aggcgagaaa cggccgtttc gcaacggtcg gttcggcttc
     3541 ggatggtgtt catgcgtaaa gcgtcgcgtc gggcggctct ggcgacggcc gggttattcg
     3601 ccctcggctc ctctctcgtc tccaacgtcg gcgccgcctc agcggcgcag cacatcgtgg
     3661 tcattcccgg ggtcgggccg ggcgatgcga cgcagccggg gcctcttccg cccgtcccgc
     3721 cggcgccgcc cgtgaagggc gcttacggcg gcggcttcat cgaattcctg atcaccggcg
     3781 attcaggcgg ccagtcggtc tcgcggccgg ctgcttcgtc ctacgcgacg ccggtctacg
     3841 ggccgccgcc cgcgccggct tattcgaacg aggcggttgc ggccctgccg gagccggcgc
     3901 ctgcaaacga gccggacctt gcggtgcgac gggagtctcc cgcgccggag ttccgccggc
     3961 aggaggtcgc ctatgacggc gatcagaagc cgggtacgat catcatcgat acgccgcgca
     4021 agttcctgtt cctggtggag ccgggcgggc gcgccattcg ctacgggatc ggcgtcggcc
     4081 ggccgggatt cgaatggtcc ggcgtgaaaa gggtcacccg aaaggccgaa tggccgggct
     4141 ggacgccgcc gccgcaaatg ttgctgcgcc gtcccgacct gccgtcgcac atggatggcg
     4201 gcctcggcaa tccgctcggc gcccgcgcgc tctatctcgg ctcgtcgctc tatcgcatcc
     4261 atggcaccaa cgagccgtgg acgatcggcc agaacgtctc atcaggctgt atcaggatga
     4321 tgaatcagga tgtcatcgat ctttacaatc gcgtacccat cggcgcgaag gtggtcgtac
     4381 tttaaacttt gaagccgcca ttgcgagcga agcgtagcaa tctcttcgtc gggcggcgca
     4441 ctacgcatgg atcgcttcgt cgctgcgctc atcgcaatga cggtggcgag ttcaagtgtc
     4501 gaaactgccg tcgttgtcgt tgccgccatc gtcgaagccg gaatcgtccg cggcgtcctg
     4561 caggtcgtcc tggtcctgat ccatgtcctg cagcgtgtct tgcgcctgct ggccggccgg
     4621 atcggcgttc tcgtagtaat tgttgacaac cgtctcgcca ccgacgccgc ctaggccata
     4681 gcctccgccg aggccatagg cgtggccgcc gaacatgccg cgcagcgcgt ccgccgccat
     4741 catgccgccg gcgacgccgg ccgccgtggt cagggcgtta tgcaggaagc cgccgccgga
     4801 agagggcggc gcgccccatg gcccggcgcc gggcggggga ccgtaattct gctgaggata
     4861 ggcttgttgc ggaccgggcg gcggcgcgct cgatcccttc gtataggcgc tcgcatcgta
     4921 attgctgcgc ggcgccggcg gcgcgctctg gccgccgccg aacaacgagc ggcccaaatt
     4981 gccgagaaag ctgccttctt gttgctctgg cggattttgg ccctggattt gcgcttcgag
     5041 ctcctgaacg cgcttcgccg cggcttcgag ggcctgttgc tggacgagga cggtctgggc
     5101 caggacgtaa ggcgcgtgcg gctgcatgcg gacggcttcg ctgatgaaat tttccgcttc
     5161 gggatcgcgc gaattggaag ccgtcgacgc gatgcgctgg aataatccgc cgagcaattg
     5221 gcgttcttgg ggggtcatag acatctccca ttcaggctga tcgcccggcc aggatatggg
     5281 cgcggccggc gccgatccaa gggcggaata acgtaaaatt tcggcaatct tttgcggacg
     5341 agacgggtca accggctttg acgccgaagc ttttcgcgag gaacaggccg ccatgccgta
     5401 ggccgccttc gtcgatgccg tgcgccacgc cgagcgacaa gtgccactgg cagggaatgt
     5461 tcgccttggc gagcgcctcc gccgattcga acagcgcctc gaacgggatg agctggtcct
     5521 gatcgccatg gacgagcaga atcggcggcg gcgcgccgcg cacatcgcga gcggccgcct
     5581 cggccagccg atcgggcccg accaggaccc ccgaatagcc gaggatcgcc gccggcgcgc
     5641 gcggccgccg caggccgaca tgcagggcca tcatggtccc ctggctgaag ccgaccaaag
     5701 cgaggcggga ttcgtcgagg tccaaacggg cgagctcggc gtcgagaaag gcgtcaagga
     5761 ccggccgcgc cttgaccacg ccgcgccaac gctcgtcggg atcgcgcatc gtcagcgcga
     5821 accattgccg ccccatgggg gacatcgcgc agggctcggg cgcatgcggc gagacgaagg
     5881 cggcgtcggg cagccaagcg cgccagtggc ggccgatttc gatcagatcg ttgccgtccg
     5941 cgccatagcc gtgcaggaag acgacgagct gcttggcctg gccgcttttc ggctcgagcc
     6001 gcggtccggt aatcagatcg gccatagacg ctccaaatct ttggcgctgt ctatcctcaa
     6061 gcgccggcga gcgccatgcc ctcccggcct tgccgcgcgg cccggtaata ggcccagagc
     6121 aggcgggcgg cgacgccgcg caatggccgc cagcgctcgg cgatcttgtg caaggctttt
     6181 gcgtccggcc gcttcttcag gccgagcgcc aggcgcgcag cctcctgcaa agcgaggtcg
     6241 ccggcgggaa aggcgtccgg atggccgagg cagaacaaaa ggaaaatatc ggccgtccag
     6301 gggccgattc ccttgaccgc gaccaaggcg cggtgcgcct cctcggcggc gagcggcgcg
     6361 agcctgtcga gatcgagcgt ccggtcttcg accgcgccgg cgagcgcgcg caaggcgcgc
     6421 attttcggcc atgagagccc gcaggcgcgc agcgcctcgt cgtcggcggc gagaacgccg
     6481 gcggcgctga gcggcgcgag attggcttcg acgcgcttga agatcgcgtt ggcgctcgcg
     6541 gtcgagactt gctgcgacac gatgatccag accaggccgg caaagcccgg ctcgcggcgg
     6601 cgcaagggcg gccgtccgcc cgcggcgagg agcttgccga tcagatcggg atcgcgcgcc
     6661 ataagctggt cgatcgcgac gttcaaggcc tcctcgccgt cgatgagcgg accgggatga
     6721 taaacgttcg acgcgggccg ccgcgcccgc cggcgcgttt tcgcttttcg cagcttggcg
     6781 ttttttgcgg aattggtccg cgcggccggt gcgccgccgc ccttgccggc gccgccgttt
     6841 ttccgaagag tgaggctcgt caccttttct cgcgtcccct atgcccaacg accagcagcg
     6901 cccggtctat cgcttcgcgc cgacgcccaa tggccgattg catctcggcc acgcctattc
     6961 ggcgctgatg aacgccaaag gcgccgagcg attcggcggc cgcttgttgc tgcgcatgga
     7021 agacttggac cgctcgcgct gcaagccgga atatgagcag gcggccatcg ccgaccttgc
     7081 ttggctcggc ctgtctttcg atccggcgat ccgccggcag agcgagcatg tttcggacta
     7141 tgcggccgcc tatcgcgtgc tggaggcgct cggtcttctc tatccgtgct tctgcacgaa
     7201 ggaggagatc gcgaaagctt cgcgcgccgc cggtttgggc cgcgacccgg acggcgcgcc
     7261 gctctatgcc ggggcctgcc gccggcgcgg atcgccgaag cgtcacggcc tggcgccgac
     7321 tttgcgcctc gatatggcgc gcgctctggc cttagccgga agcgaattgt tttggcggga
     7381 attcggcgaa ggcgacgacg agcggcgcga gcgcgccgat cccgccgcct ggggcgacgt
     7441 cgccttgaaa cggcgcggcg cgccggcgac ttatcatgtc gcggtcgtcg tcgacgacgc
     7501 gctgcaaggc gtcagcgatg tcgtgcgcgg ccgtgacctc ttcgccgcga ccggcctgca
     7561 tcggcttttg caggtcctgc tgaacctgcc gccgccgcgc tatcgccacc accgcctcgt
     7621 gctcgacgcg gaaggtgaaa aaatgtcgaa aagcgcgtct tcgacgccgc tttgccgcct
     7681 gcgcgaagcg ggcgtttcag ccgcgcagat tcgcgccgct ttggggttcg ggccggcgca
     7741 gggccggcgg atcgaggcct ggatcagtta gtcttctgcg ccagcggcgt cacgcccggc
     7801 ccggcgcttg gcgcctgcgc gaccggcgcg gcgttcggcg cctcgccgat caactggtcg
     7861 atgcggtcgc gctcgcgctt gaaggcggcg agcatcttgc cgtcgaattc gcgggtgcgg
     7921 gccagcttga tcgccatcgg gtcgacgaag cggccattga cgatgacttc gtagtggaga
     7981 tgcgggccgg tcgccatgcc ggattggccg agatagccga tcacttggcc ctgattgaca
     8041 tgcgcgcctt cgacaatgcc gcgggcgaat ccggaaagat gcgaataggt cgtcacatag
     8101 ccgttggcgt gctgaatctc gatgcgcctt ccatagccct gttcgccgcc ggctttgatg
     8161 atgacgccgt tgccggcggc cagaacgggc gtgccgggcg cgttcgccca gtcgacgccg
     8221 gtatgcatgc gcgaataatg caggatcgga tgatagcgca tgccgaacgg cgaggtcatt
     8281 tcgccggctg cgatcggctt gcggatcagg aatttgcgca ccgatcggcc gttctcgtcg
     8341 taataatcga cgcggccgtc gtccggcgtc tggaagcggt agtatttgaa cgtctggtcg
     8401 tgggcggtga acatgccgaa gagcagttcc ggccgggaat cgatgtcgtc gggctccgaa
     8461 tagaaggcct ccaccgaatc gccggcggtg acgctgcgct ggaaatcgac gtcgttggcg
     8521 aaggccttga ccatttcttc gatgatcggc ttcggcacgc cctgcttgag cacggtctgg
     8581 tagaagctct cgtagaggct catgccgccg ggcccttcag ggcttttctt ggccgccggg
     8641 ggcggcagag tgaccgcgac ataatcgccg gcgtcattgg ctgcgacagt ggccttaagc
     8701 tgctcgtcgg catagagcga gacgcgggcg attttccggg gtcctggggc gtccgtctcg
     8761 gcgaaggtca gcagcgcttt ctgtccttcg gcgatgagcc cgtcgccctt cttggccccg
     8821 aaagcggcga cgattttgac ggcgtcgggc ttcgtcgcgc cattggcggc caggagatcg
     8881 gccagggttt cgccgtgccg caagacgacc aggcgctcgg tcggctcggc cgtttcgctc
     8941 gccgggctgc gcggcacatt ggtgacgttt tccggcacca tgcgcacgtc gatcgaggcg
     9001 aaaggcgcgt tcgccccgac attgccggcc gccgcatagg cgaggccgcc gaccgggtcg
     9061 agcccggctc ggctggtgcg cgtcaacagg agctgcggcg gaatcggcgg ggcgttcttg
     9121 gcggcggtga gggcgcgcag cgattcggcg acctcggctt gcgcctcggc cgcgctcagc
     9181 cggtcggaaa tgagggccgc gtcggcggcg gtcaaatcgc gggtcgtgaa ggtcacgtcg
     9241 gcgtcgtctt gaacctggcc gggatcggtc ggctgatcgg gctcctcgat cgaggccgcc
     9301 gtcagtttga gcggattgaa ttccggcact tcgccggcga agccggtcgg cgtcgtcgtc
     9361 agggtcgtcg agacaaaggt gaaagtccgc ggcttgacga cctccttgtc gccgaccttg
     9421 atcgtcgtgg cggcgcggaa cgtctgcttg gccgcgacaa tgtcgacggg gcgcaccagg
     9481 cgatcgcctt tgccgggatt aacgccctgt tccgcctcgc ttgtcggccg agccggggcc
     9541 gcaatttcgg gggcttgggc gaagttggac tgacggtcga ggtcgagata aagggccgag
     9601 ccgaccaaag cgatgccgca aaagccggtg agaacggcgc cgctcagcca gcggaaggag
     9661 atgttgcgcc gctcgtgatg gtccggccgg cgctcgcccg cctcgatcgg ctgctcgcgg
     9721 ccaaggtcga cgacggcgcg ggaggggccg gcgcggcgct gggctaaggc gatcgtggcg
     9781 gccaaactgg tctcaagctc ccctcgtcga accgacctga cggccgcctt tttcgctttt
     9841 gatcatccgg cgcgaattga gttccgcggg cgggtcggca atcgacggcc gggtagaagt
     9901 cgcgataaac aatggcgcga atggggcgat cggctggccg gccgaatcgc ccacagaggc
     9961 cagttttcca cactcttacc gcgttccgcc caatcgcatg aacttttttc aaaaaccgtt
    10021 tgacagcctg aaaacccagt cctatatacg cgccaccgac ggcgacgacg ccttgggcgg
    10081 tcgaaaagcc atccggtttt cgcgagttta tcaggcccat gcctcccggc cgaaaggttg
    10141 gggcgaagac ggttcggttt ttttggaggc gcaagccttc ggccgtgtct tctgctgttt
    10201 gacaattgaa tcggaagaaa gagaaacgtg gacggcggag gtccttgcgg accgtcctct
    10261 cacgagggcg gtcgaagaga cactctgacg gtcacgtaaa tgagttcatc gcgttttcag
    10321 gcgaaagcct agaagcggat gtgctcggaa ctcgtcaaga gtttagtgac cagtcgggat
    10381 aaagttctca ttcaacttga gagtttgatc ctggctcaga acgaacgctg gcggcaggct
    10441 taatacatgc aagtcgaacg ggcgtagcaa tacgtcagtg gcagacgggt gagtaacgcg
    10501 tgggaacgta cccctcggtt cggaataact cagggaaact tgagctaata ccggatacgg
    10561 ccgagaggcg aaagatttat cgccgaggga tcggcccgcg tccgattagc tagttggtga
    10621 ggtaacggcc caccaaggcg acgatcggta gctggtctga gaggatgatc agccacattg
    10681 ggactgagac acggcccaaa ctcctacggg aggcagcagt agggaatatt ggacaatggg
    10741 cgaaagcctg atccagccat gccgcgtgag t
//
LOCUS       DATGJW010000070         5653 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1153322, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000070 DATGJW010000000
VERSION     DATGJW010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5653)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5653)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5653
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1153322"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..1138)
                     /locus_tag="VKU03_02105"
     CDS             complement(176..1138)
                     /locus_tag="VKU03_02105"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HLJ70080.1"
                     /translation="MPELCRMILDVDTGVDDAMAILYALNRPGIKLEALTTTFGNTDI
                     DKATANTLRILELAGRSDIPVARGPGRSLLKPFVKGADHVHGRNGLGDIVLPEPKGKP
                     VAEAASDLMIRMARENPGEITLCPVGPITNVAIALAKAPDIAPLFKEVVIMGGTIHHP
                     GIHGIPTPIADANFWNDPEAAQIVMRSGAKITLVGMDVTMKVLLTRKMRQEIAAGGRI
                     GKTMMDIADFYVRAYESMHPGMDGCGLHDPLAVAIAEDKRLATIERMCVDIELNGALT
                     RGAIVADRRRNVAPERLNADVCVDVDAERFSTMFVETMRGVGAR"
     gene            complement(1334..2296)
                     /locus_tag="VKU03_02110"
     CDS             complement(1334..2296)
                     /locus_tag="VKU03_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70081.1"
                     /translation="MSGWITPFLIHSAVQAITPILLAALAGSLCARAGVFNVALEGKI
                     LIGAFAAVVASYYTQSATGGVAAAMIAAMVFSLILAYGATIFRGDHVVICIGMNLLAS
                     GLTAYLLRVLFGVSGVFSDPAIVSLGKIRIPAIRDIPIIGWGFFSQTIVTWIAWGLVV
                     AISLILFRTPIGLRLRGVGEQPDAAETLGVNVAAYRIAAVIAAGALCGLAGAQLSLGA
                     VSVFAEDMSAGRGWIAVVAVMLGRANPIYVAGACALFGLADAMSVRLQGEGLPNQLTD
                     ISPYAVTLAALVISHRKRRRAALPSSEVDAASPGGVAEPQPDTR"
     gene            complement(2293..3327)
                     /locus_tag="VKU03_02115"
     CDS             complement(2293..3327)
                     /locus_tag="VKU03_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70082.1"
                     /translation="MTAPDAAARPRRRFRFPPAATAILFAALVGGVLVLASGANPLNA
                     YYQILAGSLSLGSLPDTLNWAAPLVGMAVAAAIPLRGGMINLGGDGQMVIGGLTSAAV
                     ALYLPAPGPVAAAAAILAAAIAAGLYASLAAWGETRHGIPMLVSSLLLSYPAVGICSY
                     IVRFPLRDTTTGLPQTRMIPEGARLLAIAGPLNIGLLIIVAIAAAAVFLDRRAIVGYE
                     LRLRGLNKRFAGYGGVDLEKQAMLAMFASGAIGGIVGAILVLGSDFRFIDEALLIPSY
                     TWSGLMAALLANGEPFGAIIAGLFFAALQTGGFAMQRETTIPRVLTLVLQAIVILFLA
                     VRHGLGRRNA"
     gene            complement(3512..5026)
                     /locus_tag="VKU03_02120"
     CDS             complement(3512..5026)
                     /locus_tag="VKU03_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70083.1"
                     /translation="MALELEAQGVVKRYGALLANDHVDLAVERGEIHAVMGENGAGKS
                     TLMSILYGLQQPDEGRILLRGAEVRFRSALDAIAAGMGMVHQAFKLFNSLSVWENIVY
                     AQEPVSGPFIDRRAARRRVADLAQRHRLGVDPDASVGALSVGVRQRIEILKALYRDAK
                     ILILDEPTAVLTPQERDGLFDVMRHLAAEGRTILFVTHKLHEVMAITGRVTVLRDGRV
                     VAKMKTSETSPREIIRAMTGRNVSLNVEKAPARLGAPLLEAIDLTVGSGRKPAVDRAN
                     LSVRAGEIVGIAGVAGNGQSELVEALVGLRSPASGAVRVAGRDVTKTNVAERRAAGIA
                     YVPEDRALVGSALPASAADNLAMGFQRNPPVARGFLIDPAAMASRAQALIAKFGVKIA
                     GARAAVGSLSGGNLQKVVVARELSHEAPVLIAEQPTRGLDVGATEFIHGQLVAERDKG
                     RAVLLISAELSEILALSDRILVMYEGKILAEVPQAKANEETLGLLMAGRIPAAA"
     gene            complement(5030..>5653)
                     /locus_tag="VKU03_02125"
     CDS             complement(5030..>5653)
                     /locus_tag="VKU03_02125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLJ70084.1"
                     /translation="EYEASFLAGAEAALTTKTGKVGAISALDIPFLHRYTDGFIEGAK
                     HVKADIKISPTLWVGGSNPFQDPARGQQRAAAMLADGADRIMAAGAGSNGGIFKAVED
                     VSGAYAFGVDVNQCVQAPGAVMDNVEKKTDIAVELAVKGIMDGKQQPITALGLKEGGM
                     TLTGLEPDVKASKCTIANYPDVIAKVKSLRDEIAGGSLKIEDPMTKK"
BASE COUNT          943 a         1878 c         1961 g          871 t
ORIGIN      
        1 agctggcgag gaattcgcaa acccaaggca agcgggcggc ttcggcttag cccgccaaaa
       61 gggtccgtca ttgcgaggcg cgcagcgacg aagcaatcca ttcgtagagc cgcgcccgcc
      121 gaatggattg cttcgctgcg ctcgcattga cggttcccaa tgcgggaagc ggccgctatc
      181 gggctcccac ccccctcatc gtctcgacga acatcgttga gaaccgctcc gcgtcgacat
      241 cgacgcagac atcggcgttg aggcgctcgg gcgcgacgtt gcggcggcgg tcggcgacga
      301 tggcgccgcg ggtcagcgcg ccattgagct cgatatcgac gcacatgcgc tcgatcgtcg
      361 ccaggcgctt gtcctcggcg atggcgacgg cgagcggatc gtgcaggccg cagccgtcca
      421 tgccgggatg catgctctca taggcgcgca cgtagaaatc ggcgatgtcc atcatcgtct
      481 tgccgatgcg gccgcctgcc gcgatctcct gccgcatttt ccgggtcagc aggaccttca
      541 tcgtcacgtc catgccgacc agggtgatct tggcgcccga gcgcatgacg atctgcgccg
      601 cctctggatc gttccagaaa ttggcgtcgg cgatcggcgt cggaatgccg tgaatgccgg
      661 gatggtggat cgtcccgccc atgatgacga cctccttgaa cagcggcgcg atgtcgggcg
      721 ccttggcgag ggcgatcgcc acattggtga tcgggccgac cgggcagagc gtgatctcgc
      781 cgggattttc gcgcgccatg cggatcatca ggtcgctcgc cgcctcggcg acaggcttgc
      841 ccttcggctc gggcagaacg atgtcgccga ggccgttgcg gccatggaca tgatcggcgc
      901 ctttgacgaa gggcttcagc agcgagcgcc cgggcccgcg cgcgacggga atgtcgctgc
      961 gcccggcaag ctccaggatg cgcagcgtgt tggccgtggc cttgtcgata tccgtgttgc
     1021 cgaaggtcgt cgtcaaagct tcgagcttta tgcccggccg gttcagcgcg taaagaatgg
     1081 ccatggcgtc gtcgacgccg gtgtcgacgt ccaagatcat gcggcagagt tcgggcatcg
     1141 cttccttcca atcgttcgct taaaaccaat ctatcaacct gcggctgacc ctcaccccag
     1201 ccctctcccg caagcgggag agggggcgcc tgtagccgcg gtcccccgac cgcagctaca
     1261 atcccttctc ccgcttgcgg aaggtggccg cgaagcggcc ggatgagggg tcaaccgcag
     1321 cgcctgccac gtgtcacctc gtgtccggct gcggctcggc gacgccgccg ggcgaggcgg
     1381 cgtcgacttc cgaggagggc agggcggcgc gcctgcgctt gcggtggctg atcaccaagg
     1441 ccgcgagcgt caccgcatag ggcgaaatgt cggtgagttg gttgggcagg ccctcgcctt
     1501 gcagccgcac gctcatcgcg tcggcgaggc cgaacaaagc gcaggccccc gcgacataaa
     1561 tcggattggc gcgcccgagc atcaccgcga ccaccgcgat ccagccgcgc ccggcgctca
     1621 tatcctcggc gaagacgctg accgcgccga gcgagagctg cgcgccggca aggccgcaga
     1681 gcgcgcccgc cgcgatcacg gcggcgatgc gataagccgc gacattgacg cccaaagtct
     1741 cggccgcgtc cggctgctcg ccgaccccgc gcaagcgcag gccgatcggc gtgcggaaca
     1801 ggatcaagct gatcgcgacg acgaggcccc aggcgatcca ggtgacgatg gtctggctga
     1861 aaaagcccca gccgatgatc gggatatcgc ggatcgctgg aatgcgaatc ttgccgaggc
     1921 tgacaatggc gggatcgctg aacacgccgc tgacgccgaa caggacgcgc aacaggtacg
     1981 cggtgagccc ggaggcgagc aggttcatgc cgatgcagat gacgacatga tcgccgcgaa
     2041 agatcgtcgc gccataggcg aggatcagcg agaagaccat ggcggcgatc atcgccgcgg
     2101 cgacgccgcc ggtcgcgctt tgcgtgtaat agctggcgac gaccgcggcg aaggcgccga
     2161 tcaggatctt gccttcgagc gccacgttga agacgccggc gcgcgcgcag agcgagccgg
     2221 cgagggcggc gagcaggatc ggcgtgatcg cctggacggc agagtgaatc aggaaaggcg
     2281 tgatccagcc gctcatgcgt tgcgcctgcc gaggccgtgg cgaaccgcca ggaagaggat
     2341 cacgatcgct tgcaagacga gggtgaggac gcgcgggatc gtcgtctcgc gctgcatcgc
     2401 gaagccgccg gtctgcaagg cggcgaagaa caggccagcg atgatcgcgc caaaaggttc
     2461 gccattggcg agcagggcgg ccatcaggcc ggaccatgta tagctcggaa tcagcaaggc
     2521 ctcgtcgatg aagcggaaat ccgagccgag caccaggatc gcgccgacga ttccgccgat
     2581 cgcgccgctc gcgaacatcg ccagcatcgc ctgcttttcg aggtcgacgc cgccatagcc
     2641 ggcgaagcgc ttattgaggc cgcgcaggcg cagctcatag ccgacgatcg cgcgccgatc
     2701 gaggaacacc gcggcggcgg cgatggcgac gatgatgagg aggccgatat tcagcggccc
     2761 ggcgatcgca agaagccgcg ccccttcggg gatcatccgc gtctgcggca ggccggtcgt
     2821 cgtgtcgcgc agcgggaagc gcacgatgta cgagcagatg ccgacggccg ggtagctcag
     2881 cagcaggctc gacaccagca tcggaatgcc gtggcgggtc tcgccccagg cggcgagcga
     2941 ggcgtagagg ccggcggcga tcgcggcggc gaggatcgcg gccgccgccg cgaccgggcc
     3001 gggcgccgga aggtacaagg cgaccgccgc cgaggtgagg ccgccgatca ccatctgtcc
     3061 gtcgccgccg agattgatca tgccgccgcg cagcggaatc gccgccgcga ccgccatgcc
     3121 aaccagcggc gccgcccaat tcagcgtgtc gggcagggag ccgaggctca gcgagccggc
     3181 gaggatttgg taataggcgt tgagcggatt ggcgccggag gcaaggacga gaacgccgcc
     3241 gacgagagcc gcgaagagga tcgccgtcgc ggcgggcggg aagcggaagc gccggcgcgg
     3301 ccgggcggct gcgtcgggcg cggtcatgga ggcggctcga agccaatcct tccccgcgaa
     3361 gcgggggagg gggaccgcgc tttagcgcgg tggagggggc tgcggcgttt gggattcctc
     3421 ctgatgcggc gcgcgccccc tccaccatgc ttcgcatggt ccccctcccc cgcttcgcgg
     3481 gggaggatag tccagcccga gcccgccgcg ctcatgcggc ggccgggatg cggccggcca
     3541 tcagcaggcc gagcgtttcc tcattcgcct tggcttgcgg cacctcggcg agaatctttc
     3601 cttcgtacat gacgagaatc cggtcgctca gcgcgaggat ttcgctgagt tcggccgaaa
     3661 tcagcaggac ggcgcggccc ttgtcgcgct cggcgacgag ctggccatgg atgaattccg
     3721 tcgccccgac atcgaggccg cgggtcggct gctcggcgat caaaaccggc gcctcgtgcg
     3781 aaagctcgcg cgcgaccacc actttctgga gattgccgcc ggagagcgag ccgacggcgg
     3841 cgcgcgcgcc ggcgatcttg acgccgaact tggcgatcag cgcctgcgcc cggctcgcca
     3901 tggcggcggg atcgatcagg aagccgcggg cgaccggcgg gttgcgctga aagcccatgg
     3961 cgagattgtc ggcggcgctc gccggcaaag ccgagccgac cagcgcgcga tcctccggca
     4021 cataggcgat cccggcggcg cggcgctcgg cgacattggt cttggtcacg tcgcggccgg
     4081 cgacgcgcac cgcgccggaa gcggggcttc tcaggccgac cagcgcctcg acaagctcgc
     4141 tctggccatt gccggcgacg ccggcaatgc cgacgatctc gccggcgcga acgctgagat
     4201 tcgcccggtc gaccgccggc ttgcggcccg agccgaccgt cagatcgatc gcctcgagca
     4261 gcggcgcgcc gagccgcgcc ggcgccttct cgacattcag gctgacattg cgcccggtca
     4321 tcgcccggat gatttcgcgc gggctcgtct cgctcgtctt catcttggcg acgacgcggc
     4381 cgtcgcgcag cacggtgacg cggccggtga tcgccatcac ctcatgcagt ttgtgcgtga
     4441 cgaagaggat cgtccggcct tcggcggcga gatggcgcat cacgtcgaaa agcccgtcgc
     4501 gctcctgcgg cgtcagcacc gcggtcggct cgtcgaggat gagaatcttg gcgtcgcgat
     4561 agagggcttt caggatttcg atgcgctggc gcacgccgac cgaaagcgcg ccgaccgagg
     4621 cgtcgggatc gacgccgagg cgatggcgct gggcgagatc ggcgacacgc cgccgcgccg
     4681 cgcgtcggtc gatgaacggg ccgctcaccg gctcctgagc atagacgatg ttctcccaga
     4741 ccgagagcga gttgaacaat ttgaacgcct ggtgaaccat gcccatgccg gcggcgatcg
     4801 cgtcgagcgc cgagcggaaa cggacctcgg cgccgcgcag caggatgcgg ccctcgtcgg
     4861 gctgctggag gccgtaaagg atgctcatca gcgtcgactt gccggcgccg ttctcgccca
     4921 tgaccgcgtg aatctcgccg cgctcgacgg cgaggtccac atgatcattg gcgagcagcg
     4981 cgccgtagcg cttgacgacg ccctgcgcct cgagttcgag cgccaccctc tacttcttcg
     5041 tcatcgggtc ttcgattttc agcgagccgc cggcgatttc gtcgcggagc gatttcacct
     5101 tggcgatcac gtcgggatag ttggcgatcg tgcatttcga cgccttcacg tccggctcaa
     5161 gcccggtcag cgtcatgccg ccttccttca agccgagggc cgtgatcggc tgttgcttcc
     5221 cgtccatgat gcccttgacg gcgagctcga cggcgatgtc ggtcttcttt tcgacattgt
     5281 ccatcaccgc gcccggcgcc tgcacgcatt gattgacgtc gacgccgaac gcataggcgc
     5341 cggaaacatc ctcaaccgcc ttgaagatgc cgccattcga gccggcgccg gcggccatga
     5401 tgcggtccgc gccgtcggcg agcatggcgg cggcgcgctg ctggccgcgc gccggatcct
     5461 ggaacggatt ggagccgccg acccatagcg tcggagaaat cttgatgtcc gccttcacat
     5521 gcttggcgcc ttcgatgaag ccgtccgtgt agcgatgcag gaagggaata tcgagcgcgc
     5581 tgatcgcgcc gaccttgccg gtcttggtcg tcaaggccgc ctcggcgccg gccaggaagc
     5641 tcgcctcata ttc
//
LOCUS       DATGJW010000071         7580 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_842834, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000071 DATGJW010000000
VERSION     DATGJW010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7580)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7580)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7580
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_842834"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..717
                     /locus_tag="VKU03_02130"
     CDS             <1..717
                     /locus_tag="VKU03_02130"
                     /inference="COORDINATES: protein motif:HMM:NF017853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF937 domain-containing protein"
                     /protein_id="HLJ70085.1"
                     /translation="MQAAQGGQGVNNLASQFGLTPDQAQSAVQAVIPAFSQALQRMGQ
                     DPNALGGVLAHIASGAHQGSFADPSQAAASAQAGGNVLGQIFGSPQVSANISQQASRV
                     SGVSPEIIQAMLPVVASMLMGGLMHSMNSQGLGGVLGQLANAVTASGGLGSVLEGGAG
                     QAGQSGGGLGGLVGSVLGGLLGGGQSSASPQSSTLQTGLNSLINMFESGVQANPAHEQ
                     GISNILDSLGAQFAQGGARS"
     gene            complement(733..1374)
                     /locus_tag="VKU03_02135"
     CDS             complement(733..1374)
                     /locus_tag="VKU03_02135"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007699252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HLJ70086.1"
                     /translation="MKIRFAAFALVFISTAAPAAAQTIIDDWAKVKAPPAPELKSVTL
                     DAKTAALLLLDFNGHDEATSGPCNKATKPRCLASLPPVRKLLERAREKGVFVVYSLSP
                     NGAPGDIRPAIAPTPDEPIVKSGPDKFLRTDLGELLTGRGIKTVIVAGTASEGAVLDT
                     GTDAALHGMNVVVPVDGMSSTDLYAEQYVAWHFTHAPGVSQKSTLSRTDLIGF"
     gene            complement(1475..2383)
                     /locus_tag="VKU03_02140"
     CDS             complement(1475..2383)
                     /locus_tag="VKU03_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ70087.1"
                     /translation="MNIHDLEAFVAVVESGSIVGASAKLNLTQPGVTRRVQNLEDALG
                     AALLDRQSKPLKPTHAGREAYEQGRRALRALENLKASVSPTGEIRGEFRLGIMPYLSE
                     AALAAPLDALRGAFPQLTLRIVSGWSTRLIEQVLRSELDAAALCLADGLNPPDGLVSE
                     NLGTQQVLLVAARSLKVPKPATLADLSKFPWVMNENGCGFRAFMRHSFEAARLPFHVG
                     VEAISADLRLSLVARGLGIGILTPAAFAKRERREEVELVESPDFRPRVRAWMLHRPPA
                     GRLARPIEVFRDELLEALKSPAAFAS"
     gene            2554..3708
                     /locus_tag="VKU03_02145"
     CDS             2554..3708
                     /locus_tag="VKU03_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011086739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70088.1"
                     /translation="MALFAIATGVIVTNLFAPQTLIGLIGATLRLSPAAGGFAAMTTL
                     LGYAAGLFLLVPLADLRENRRLVVIMIAAAAASALAAALASNGGALLAALFGLGAASS
                     AIQILVPLAAAMASADTRGRVIGDVMGGLMIGILLSRPIASAIADAYDWRAFYGLSAA
                     AMAILAISLAALLPERKPSQSTSYAALIASLWSLLRAEPVLRRRALTASLSMAAFSLF
                     WTAVALRLAQQPFVLSQKGIAIFALVGVGGALATPLAGRAGDRGWTRAATILAHTAIF
                     AAFALAAWAGLSRADAALRLVLMAVAAVLLDIGVTGDQTLGRRSVNLLQPEARGRLNG
                     LFVGLFFLGGALGSAVVGAAWTQGGWTMICCVGGGFGLLALIVDILSKRD"
     gene            complement(3767..5866)
                     /locus_tag="VKU03_02150"
     CDS             complement(3767..5866)
                     /locus_tag="VKU03_02150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HLJ70089.1"
                     /translation="MTHPSENLDAAAPRAERKPERRTAHGRAWTDDYAWLRAENWREV
                     LRDPKQLPAPIRRHLDAENAYSAEILGPFKALKRELAREMRARLKEDDSEAPQPDGPY
                     AYYTRYRHGGQHRIFCRIPRGGGEESVLVDGDAVAAGKTFFHLFDARHSPDHSKLAWS
                     ADGKGSELFAIRVRDIASGADLDDRVENCTGHAVWTRDSSAFLYVAVDENHRPWRVLL
                     HRLGEAASADRVIFEEADPAWFINVSPTRLGRLAIISVHGHDSSESHVVDLDQPAAAP
                     RVIEPRRPGLRYDVMDHGDRFFIRANSGGAADFKIVVAPAEAPGMANWRDLIPHKAGR
                     LIVATALFKRFLARLEREDGLPRIVVRDLETDAEHRVAFEAEAYSLGFENVFEFDQGV
                     MRFSYSSMTTPREIYDYDMATRERTLRKRQMAPKGFQSSDYVTRRVFATAPDGESVPI
                     SLLHRKDTPIDGTAPLLIYGYGAYGLAIDAAFSTNRLSLVDRGFVFAIAHVRGGTDKG
                     WRWYEDGKLAKKTNTFGDFIAATEHLVASGFAAKDKVVAQGGSAGGMLMGAIANRAPG
                     LYRAIIADVPFVDVLTTMLDASLPLTPPEWLEWGNPILDQEAFETIRSYSPYDNIEAR
                     RYPAILAMAGLTDPRVTYWEPAKWIAKLREIATGGPFLLHVNMGAGHGGASGRFDQLE
                     DVALGYVFALGAISGEL"
     gene            6069..6713
                     /locus_tag="VKU03_02155"
     CDS             6069..6713
                     /locus_tag="VKU03_02155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007528984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FMN-binding negative transcriptional regulator"
                     /protein_id="HLJ70090.1"
                     /translation="MYEPLHHKKDDLAAQHALIRAQPLGLLISHGPDGLEANAIPFLI
                     DASASKLGTLRAHMARANKQWRALQQAEEALVVFQGPDRYISPNWYATKRETGKVVPT
                     WNYVMVQAAGRPRVIEDPEWLRAQIGELTRAHEGVRPAPWAVSDAPADFVAMQIKAIV
                     GVEIEIARIAGKWKASQNRPAADRDGVVQGLIAEGEPMALDMADIVRKEGGPKA"
     gene            6946..7419
                     /locus_tag="VKU03_02160"
     CDS             6946..7419
                     /locus_tag="VKU03_02160"
                     /inference="COORDINATES: protein motif:HMM:NF039330.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6036 family nucleotidyltransferase"
                     /protein_id="HLJ70091.1"
                     /translation="MYPDFKELLSAFNDNAVKYLVVGGYAVSFHAQPRATKDLDIFLG
                     PSADNAAAAYRALVQFGAPIAALKPEDLVEPGKFFRMGTPPVMVGIMPEIIGIAFDDA
                     WSRRVEAVIDPDLQLKAPIVSKGDLIAAKLAAARPQDLADVDAIEKAERDRTSGD"
BASE COUNT         1263 a         2443 c         2532 g         1342 t
ORIGIN      
        1 atgcaggcgg cgcagggcgg ccagggcgtc aacaatctgg cgtcgcaatt cggcctgacg
       61 cccgaccagg cccagtcggc ggtgcaggcg gtcattcccg ccttttcgca ggccttgcag
      121 cggatggggc aggacccgaa cgcgctcggc ggcgtgctcg cccatatcgc cagcggggct
      181 catcaaggtt ccttcgccga tccgtcgcaa gccgccgcct cggcgcaagc cggcggcaac
      241 gtgctcgggc agattttcgg ctcgccgcaa gtctccgcca atatcagcca gcaggcctcg
      301 cgcgtttccg gggtcagccc tgaaatcatc caggccatgc tgccggtcgt cgcctcgatg
      361 ctgatgggcg gcttgatgca ttcgatgaat tcgcaaggcc tgggcggcgt tctgggccag
      421 ttggcgaacg ccgtgaccgc ttccggcggc cttggctcgg tgctcgaagg cggcgcgggc
      481 caagccgggc aaagcggcgg cggcttgggc ggccttgtcg gcagcgtcct tggcggcttg
      541 ctgggcggcg gccagagcag cgccagtccg caaagctcga cgctgcaaac cggcctcaac
      601 tcgctgatca atatgttcga atccggcgtt caggccaatc cggcgcatga gcaggggatc
      661 agcaacattc tcgattcgct cggcgcgcaa ttcgcgcagg gcggcgccag aagctgaggc
      721 gccgcgcggc gttcaaaagc cgatcaggtc agtccggctc agcgtggatt tctggctgac
      781 ccccggcgcg tgggtgaaat gccaagcgac atattgctcg gcatagagat ccgtcgacga
      841 catgccgtcg accggaacga cgacattcat gccgtgcagg gccgcgtccg tcccggtgtc
      901 gagcaccgcg ccttccgagg cggttccggc gacgatgacc gtcttgattc ccctgccggt
      961 cagaagctcg ccgagatcgg tgcgcagaaa tttgtccggg ccggatttga cgatcggctc
     1021 gtcgggcgtc ggcgcgatgg ccggccggat gtcgccgggc gccccgttcg gactgaggct
     1081 gtagacgacg aaaacgcctt tttctcgcgc ccgttcgagg agcttgcgaa caggcggcag
     1141 cgaagcgagg cagcgcggct tggtcgcctt gttgcaaggg ccgctcgtcg cctcgtcatg
     1201 gccgttgaag tcgagcagca gcagcgccgc ggtcttcgcg tcgagcgtga ccgatttcag
     1261 ctccggcgcg ggcggcgctt tcactttcgc ccagtcgtcg atgatcgtct gcgcggcggc
     1321 gggagccgct gtggagatga aaaccagcgc gaaagctgcg aaacgaattt tcatcaggcg
     1381 atcctcccga tttcgacgcc tcgcgcggct atcctggatt ctccaacgcc gatttgcgat
     1441 cacgatcgcg cgacctgatc tgtgcgcccg gcattcacga tgcgaacgcg gcaggagatt
     1501 tcagcgcctc aagcagttca tcgcgaaaaa cttcgatcgg ccgcgccagg cggccggccg
     1561 gcggccgatg cagcatccag gcgcgcactc gcggccgaaa atccggcgat tcaacgagtt
     1621 cgacctcctc ccgccgctcc cgctttgcga aagcggcggg cgtgagaatg ccgatgccga
     1681 gcccgcgcgc gaccagcgat agacgcaagt cggcgctgat cgcctcaacg ccgacatgga
     1741 aaggcagccg agcggcttcg aaactatgcc gcatgaaagc gcgaaagccg cagccgttct
     1801 cgttcatcac ccaaggaaat ttagacaggt cggcaagcgt cgcgggcttc ggcactttga
     1861 ggctgcgcgc cgcgacgagc agcacctgct gggtccccag gttttcgctg acgaggccat
     1921 cgggcgggtt gagcccgtcg gccaggcaca gagccgcggc gtcgagctcg ctgcgcagga
     1981 cttgctcgat gagccgcgtc gaccagcctg agacgatgcg aagcgtcagt tgcggaaacg
     2041 cgccgcgcaa tgcgtcgagc ggcgccgcca aagcggcctc ggaaagatag ggcatgattc
     2101 cgagccgaaa ctcgccgcgg atttcgccgg tcggcgaaac gctggccttc agattctcca
     2161 gagcgcgcag cgcgcgccga ccttgctcat aggcctcgcg tccggcgtgc gtgggcttga
     2221 gcggctttga ttggcggtcg agcagcgccg cgccaagcgc gtcctcgaga ttttgcacgc
     2281 ggcgcgtgac gccgggctgg gtcagattga gcttcgctga agcgccaacg atcgagccgg
     2341 attccacaac ggccacaaag gcttcgagat cgtggatgtt catatcagac tcgcataaac
     2401 atcataaata gaattcaatt gtatcatgat tacggcaggc aataaagctc tccatcgata
     2461 tgcgatttca gcaatcgaag gagaccccat gtccacgccg acccgttctg cctcgcagcg
     2521 cagccagccg ctgcggtcgg cccccggccg cctgtggctc tattcgccat cgcgacgggc
     2581 gtgatcgtca ccaatctttt cgctccgcag accttgatcg gtctcatcgg cgcaacgctc
     2641 cgcctttcgc cagccgccgg cggctttgcc gcgatgacga cgctgcttgg ctacgcggcg
     2701 ggcttgtttc tgctggtccc gcttgccgac cttcgcgaga atcgacgcct cgtcgtgatt
     2761 atgatcgccg ccgcggcggc aagcgccctc gccgccgcct tggcgtcgaa tggcggcgcg
     2821 cttttggccg cgctgtttgg tctcggcgcg gcgtcatccg ccattcagat ccttgttccg
     2881 ctcgccgccg cgatggcttc cgccgatacg cgcggccgcg tcatcggcga cgtcatgggc
     2941 ggcttgatga tcggcatact cttgtcgcgg ccgatcgcca gcgccattgc cgatgcgtac
     3001 gattggcgcg ccttctacgg tttgagcgcc gccgcgatgg ccatcttggc catctcgctc
     3061 gccgcgcttt tgcccgagcg aaagccctcg cagtccacga gctacgcggc gctcatcgcc
     3121 tcgctgtgga gcctgttgcg ggcagagccg gttctgcgcc ggcgcgcgct gaccgcgagc
     3181 ctttccatgg cggcgttcag cctattctgg acggcggtcg cgctcaggct tgcgcagcag
     3241 cccttcgtcc tgagccaaaa gggaattgcg atcttcgcgc tggtcggcgt cggcggcgcc
     3301 ttggcgacgc cgctcgccgg acgcgccggc gatcgcggct ggacgcgcgc cgcgaccatc
     3361 cttgcgcata ccgcaatctt cgccgcattc gcgctcgcgg cctgggcggg attgtcgcga
     3421 gccgatgcgg ccctccggct cgttctcatg gcggttgcgg cggtcttgct cgatatcggc
     3481 gtcaccggcg atcagaccct cggccgccgc tcggtcaatc tgctccagcc ggaggcgcgc
     3541 ggtcggctca acgggctgtt tgtcggcttg ttctttcttg gcggcgcgct tggctcggcg
     3601 gttgtcggcg cggcttggac gcagggcgga tggacgatga tttgctgcgt cggcggcggg
     3661 tttggcctgc tcgccttgat cgtcgatatt ttaagcaagc gagactgacg cgcaaagacg
     3721 tagccctgcg agcggaccgg ttcgctccgc tcgcaatgat ggtacgctac aactccccgc
     3781 tgatcgcacc gagcgcgaag acgtagccca aagcgacgtc ttcgagctgg tcgaagcggc
     3841 ccgaggcgcc gccatggccg gcgcccatat tgacatggag caggaacggc ccgcccgtgg
     3901 cgatctcgcg cagcttggcg atccatttcg ccggctccca ataggtaacg cgcggatcgg
     3961 tcagcccggc catggcgaga atcgccggat agcggcgcgc ctcgatattg tcgtagggac
     4021 tgtaggatct gatcgtctcg aaggcctctt ggtcgaggat cgggttgccc cattcgagcc
     4081 attcgggcgg cgtcagcggc aggctggcgt cgagcatggt ggtcagcacg tcgacgaagg
     4141 gcacgtcggc gatgatcgcc cggtagaggc ccggcgcccg gttggcgatc gcgcccatga
     4201 gcatgccgcc agcgctgccg ccttgcgcga cgaccttgtc cttggcggcg aagccgctgg
     4261 cgacgagatg ctcggtcgcg gcgatgaaat cgccgaacgt gttggtcttc ttggcgagct
     4321 tgccgtcctc gtaccagcgc cagcccttgt cggtcccgcc gcgcacatgg gcgatggcga
     4381 agacgaagcc acggtcgacc agcgacaggc ggttggtcga gaaagccgcg tcgatcgcca
     4441 ggccataggc gccgtagcca tagatcagca gcggcgccgt gccgtcgatc ggcgtgtcct
     4501 tgcgatgcag gagcgagatc ggcacgctct cgccgtccgg cgccgtggcg aagacgcgcc
     4561 gcgtgacgta gtcgctcgat tgaaagccct tcggcgccat ctggcgcttg cgcaaggtcc
     4621 gctcgcgcgt cgccatgtcg tagtcgtata tctcgcgcgg cgtcgtcatc gacgaatacg
     4681 agaagcgcat cacgccctgg tcgaattcga agacgttctc gaagccgagc gaataggcct
     4741 ccgcctcgaa ggcgacgcga tgctccgcgt cggtctcgag atcgcgaacg acgatgcgcg
     4801 gcaggccgtc ctcgcgctcg agccgcgcca ggaagcgctt gaacaaggcc gtcgcgacga
     4861 tcagccggcc ggccttgtgc gggatgagat cgcgccaatt ggccatgccc ggcgcctcgg
     4921 ccggcgcgac gacgatcttg aaatccgcgg cgccgccgct attggcgcgg atgaagaagc
     4981 ggtcgccgtg atccatcacg tcgtagcgca ggcccggccg gcgcggctcg atcacgcgcg
     5041 gcgcggcggc cggctggtcg agatcgacga cgtggctttc cgacgaatcg tggccatgga
     5101 cggagatgat cgccaatcgc ccgagccggg tcggcgagac attgatgaac caggccgggt
     5161 cggcctcttc gaaaatcacc cgatcggcgc tcgcagcctc gccgagccgg tgcagcagca
     5221 cccgccaggg gcgatggttc tcgtcgacgg cgacatagag aaaggccgag gagtcgcgcg
     5281 tccagacggc atggccggtg caattctcga cgcggtcgtc aagatcggcg cccgaggcga
     5341 tgtcgcgcac gcggatcgcg aacagctcgg agcccttgcc gtcagcgctc caggcgagct
     5401 tgctgtggtc cggcgaatgg cgcgcgtcga agagatgaaa gaacgtcttc ccggcggcca
     5461 ccgcgtcgcc gtcgacaagg acgctctcct cgccgccgcc gcgggggatg cggcagaaaa
     5521 tccgatgctg cccgccatgg cgatagcgcg tgtaataggc ataggggccg tcgggctgcg
     5581 gcgcctcgct gtcgtcctcc ttgagccggg cgcgcatttc gcgcgccagc tcgcgcttta
     5641 acgccttgaa gggaccgaga atttcggcgg aataggcgtt ctcggcgtcg agatggcggc
     5701 ggatcggcgc cggcaattgt ttcgggtcgc gcaggacctc gcgccaattc tccgcccgca
     5761 gccaggcgta atcgtcggtc caggcgcggc catgcgccgt ccgccgttcg ggcttccgtt
     5821 ccgccctcgg cgccgccgcg tccaagtttt cggaagggtg cgtcatcagt ctttcgagca
     5881 tgggcggcgt tgaagcccgg ctgagggggc tttgtccagg cctcggcctg ctcatgcttg
     5941 ccatgcgccg ggctcatggg aaagcctgcc cgcgggcggc gattgtgtcg gggcgatcgg
     6001 gaggcatata gatcggcccg cccggcgaga gggcggccac gcgctccggg cgctgcggat
     6061 tgaaaccgat gtatgagccc ctgcaccata agaaggacga cctcgccgcc cagcatgcgc
     6121 tgatccgggc ccagccgctc ggcctcctga tcagccacgg tccggacggg ctcgaggcca
     6181 atgcgattcc tttcctcatc gacgcctcgg cctcgaagct cgggacgctg cgcgcccata
     6241 tggcgcgggc gaacaagcaa tggcgcgcct tgcagcaggc ggaggaggcg ctcgtcgtgt
     6301 ttcaggggcc ggaccgctac atctcgccga actggtacgc gaccaagcgc gagaccggca
     6361 aggtcgtgcc gacctggaat tacgtgatgg tgcaggccgc cggccggccg cgcgtcattg
     6421 aagatcccga atggctgcgc gcgcagatcg gcgagctgac gcgcgcgcat gaaggcgtgc
     6481 ggcccgcgcc ctgggcggtc tccgacgcgc cggccgattt tgtcgcgatg cagatcaagg
     6541 cgatcgtcgg cgtcgagatc gagatcgccc gcatcgccgg caaatggaaa gcgagccaga
     6601 accgccccgc cgccgaccgc gacggggtgg tccaaggcct gatcgccgaa ggcgagccga
     6661 tggcgctgga catggcggat atcgtgcgca aggagggcgg acctaaagct tgaaaggccc
     6721 tttcgattct gccgaaaatc cgttttcggg tataatcgcg gctgccggat aaggaattgc
     6781 ccctcgtgga aagaagagcc cgtttttcaa tcaaacgtta cgacagcgtg gacgccatga
     6841 aggcggacga atacgcctat tggcgagagc gctccccgca agaacgcttc gcggccgtct
     6901 ccgaaatttc aagtgaggct tacggcttga aagaccctgg cgcgaatgta ccccgacttc
     6961 aaagaactct tgtccgcctt caacgataac gccgtaaaat atctcgttgt tggcggctac
     7021 gccgtctctt ttcatgcgca gcctcgggct acgaaggatt tggatatttt tcttggtccg
     7081 agcgccgaca acgcggcggc cgcatatcgc gcgttggtgc agtttggcgc cccaatcgcg
     7141 gcgcttaagc cggaagatct ggtagagcca ggcaaattct tccgcatggg aacgccgcct
     7201 gtcatggtgg gtatcatgcc ggaaatcatc ggcattgctt ttgatgacgc gtggtcgcgg
     7261 cgtgtcgagg ccgttataga ccctgatcta caattgaaag cgccgattgt ctccaaaggc
     7321 gacttaatcg cggcaaagct cgcagcggcg cgaccacaag accttgcaga cgtcgatgcg
     7381 atcgagaagg ccgaaaggga tcgcacaagt ggcgattgag aaagctccct tttgtgtcgc
     7441 gcgataatgg agaaggggcg gcctcgcgct tgcggacgcg ttgaaaattg caaagggatc
     7501 gtccgcgcgc ccgcggccta aggcggcagg ccacccttcc gcaggccgtc gagtaaatgg
     7561 tcgcggtcgg cggcgaattt
//
LOCUS       DATGJW010000072         4919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_116961, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000072 DATGJW010000000
VERSION     DATGJW010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4919
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116961"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VKU03_02165"
     CDS             <1..958
                     /locus_tag="VKU03_02165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ70092.1"
                     /translation="DEVVVMQRGRIVEQGQVGKVFANPQHPYTKMLLAAEPKGRAPAL
                     DIAAPAVVEAKNIKVWFPIKRGFLRRTIGHVKAVDGVTVAVREGQTVGVVGESGSGKS
                     TLGLAILRLIRSEGVVIYAGKRIDGLKMKAMRPFRRDMQVVFQDPYGSLSPRLSIAEI
                     VEEGLIVQRKGMKAAARREAVARALADTGLDPAAMDRYPHEFSGGQRQRIAIARAMAL
                     DPKFVVLDEPTSALDMSVQAQIVDLLSDLQKKRSLAFLFISHDLKVVRALASEIIVMR
                     QGKVVESGPAEQIFAAPQEDYTKALFKAAFNIEADTSGAVSQ"
     gene            1141..2358
                     /locus_tag="VKU03_02170"
     CDS             1141..2358
                     /locus_tag="VKU03_02170"
                     /EC_number="5.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008973 - phosphopentomutase activity
                     [Evidence IEA];
                     GO_process: GO:0009117 - nucleotide metabolic process
                     [Evidence IEA];
                     GO_process: GO:0043094 - cellular metabolic compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopentomutase"
                     /protein_id="HLJ70093.1"
                     /translation="MGRALLIVLDSVGCGGAEDAAAYGDDGADTLGHIAEACARGEGD
                     RKGLRRGPLHLPNLDRLGLGRAMAASTGRKPPGLGESGPIGHWGYGVETSLGKDTPSG
                     HWEIAGAPVRFQWGYFPKEAPAFPPGLTATLIEQARLPGILGDKHASGTEVIEEFGEE
                     HLRTLKPICYTSADSVLQIAAHEEAFGLDRLYDLCRIARRLCDPLKIGRVIARPFVGR
                     RAGEFSRTPRRKDFAIPPPPGNLFERAAKAGRDIVSVGKIGDIFAHSHTGREVKGADN
                     MAHVDLALQALASVADGGLIFVNLVDFDTEYGHRRDVAGYAACLEAFDRRLPEIIAAM
                     RPDDYGIVTADHGNDPTWRGFDHTREHVPILGFGDGVKPAPIGRRKTLADIAQTLSKK
                     LGLPPGPNGESWI"
     gene            2363..2803
                     /locus_tag="VKU03_02175"
     CDS             2363..2803
                     /locus_tag="VKU03_02175"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331571.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HIT family protein"
                     /protein_id="HLJ70094.1"
                     /translation="MSGFALDPRLAADTLALGDARLSRVLLMNDARFPWLILVPRRAG
                     LAEIAELSPNERETLMEEIVAVSNALSQRQGVEKINVGALGNVVRQLHVHIVGRSRDD
                     AAWPGPVWGSGARQPYQNAAAQQLLRELAQPIAHILIAASDESG"
     gene            2948..3925
                     /gene="sppA"
                     /locus_tag="VKU03_02180"
     CDS             2948..3925
                     /gene="sppA"
                     /locus_tag="VKU03_02180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptide peptidase SppA"
                     /protein_id="HLJ70095.1"
                     /translation="MTIPSSSVDYVVDRRRMRRQATFWRAAAFVILVLAIIALGWRYS
                     GLPTGSRLESHIARVSITGLITGDEASLKLIRDLRDSNAKAVLLSISSPGGTTVGSER
                     LYEALRRLAAKKPVVAVVDNMAASGAYIAALGADRIFAEGNSLVGSIGVLVQYPNFSG
                     LLDKIGVKYEAIKSSPLKAAPNGFEPTSEEARAAMASLVSDSYDWFKKLVKDRRGLSD
                     SELAAVDDGRVFTGRQGLSLKLIDAIGGEREAIAWLEKDKGVAKNLPILDWKPQRSLG
                     GFGFFGSAAAAADWLGMTNLGALLRQSELAGEGQLLDGLVSIWQLPDRD"
     gene            3979..4269
                     /locus_tag="VKU03_02185"
     CDS             3979..4269
                     /locus_tag="VKU03_02185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005694 - chromosome [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integration host factor subunit beta"
                     /protein_id="HLJ70096.1"
                     /translation="MIKSELVQRMSARNPHLYQRDVENIVDAILDEITAALARGERVE
                     LRGFGAFSTKQRAARTGRNPRTGDQVPVIEKVVPFFKTGKEMRERLNKGANG"
     gene            4283..4585
                     /locus_tag="VKU03_02190"
     CDS             4283..4585
                     /locus_tag="VKU03_02190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385846.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LapA family protein"
                     /protein_id="HLJ70097.1"
                     /translation="MLRVAKFVVLALVAVVLLLFAFANRQIVSVSFDPFASAEAPAFA
                     LAAPLFLVLMLTLILGVIVGGAAVWLNQSRFRRAARQSRAEIERLRAQSANVPSVH"
     gene            4618..>4919
                     /locus_tag="VKU03_02195"
     CDS             4618..>4919
                     /locus_tag="VKU03_02195"
                     /EC_number="4.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014766549.1"
                     /note="catalyzes the formation of L-proline from
                     L-ornithine; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HLJ70098.1"
                     /translation="MQVIDAKAVDERLDFPALIDALAEAFRGAFKAPARVHHQIERAG
                     AAATHLLMPAWTTSAYRQGAFLGTKIVSVFPDNAARGLPAVHGVYVLQSGETGAPLA"
BASE COUNT          817 a         1626 c         1597 g          879 t
ORIGIN      
        1 cgatgaggtc gtggtgatgc agcgcggccg catcgttgaa caaggccagg tcggcaaagt
       61 cttcgccaat ccgcagcatc cttatacgaa aatgctcctc gccgccgagc cgaaaggccg
      121 cgcgccggcg ctcgacatcg ccgcgccggc ggtggtcgaa gccaagaaca tcaaggtctg
      181 gtttccgatc aagcgcggct tcctgcgccg cacgatcggt catgtgaagg cggtcgacgg
      241 cgtcaccgtc gccgtccgcg aaggccagac cgttggcgta gtcggagagt ccggctccgg
      301 caagagcacg ctcggcctcg ccattttgcg gctgatccgc tcggaaggcg tcgtcattta
      361 cgccggcaag cggatcgacg ggctcaagat gaaggcgatg cgtcccttcc ggcgcgacat
      421 gcaggttgtg ttccaggacc cctacggctc gctgtcgccg cgcctgtcca tcgccgaaat
      481 cgtcgaagaa gggctgatcg tgcagcgcaa gggcatgaag gccgcggcgc ggcgcgaagc
      541 ggtggcgcgg gcgctcgccg ataccggcct cgatccggcc gcgatggacc gctacccgca
      601 cgaattttcc ggcgggcagc gtcagcgcat cgccattgcc cgcgccatgg cgctcgatcc
      661 gaaattcgtg gtgctcgacg agccgacctc ggcgctcgat atgtcggtgc aggcgcagat
      721 cgtcgaccta ttgagcgact tgcagaaaaa gcgctcgctg gcctttctct tcatctcgca
      781 cgatctgaaa gtggtgcggg cgcttgcttc cgagatcatc gtcatgcggc aaggcaaggt
      841 cgtcgaaagc ggcccggccg agcagatttt cgccgcgccg caggaggatt ataccaaggc
      901 cttgttcaag gcggcgttca acatcgaggc ggatacgagc ggcgcggtga gtcagtaaag
      961 gagctatcct cccccgtgaa acggcggagg gagaccgcgc gcagcgcggt ggagggggcc
     1021 gccggcggtt gagattatcc cgagagaccg taccccctcc accatgctcc gcatggtccc
     1081 cttcccccgc ttcgcggggg aggattggcc gccgcccaca aacaatgcaa aggaacatcc
     1141 atggggcgcg cgcttctcat cgtgcttgac tcggtcggct gcggcggcgc ggaggatgcg
     1201 gcggcgtatg gcgacgacgg cgccgatacg ctcggccaca tcgccgaggc ctgcgccaga
     1261 ggcgaaggcg accgcaaggg cctgcgccgc ggaccgcttc atctgcccaa tctggatcgg
     1321 ctcggcctcg gccgcgccat ggcggcctcg accgggcgca agccgccggg cctcggcgag
     1381 agcgggccga tcggccattg gggctatggc gtcgaaacct cgctcggcaa ggatacgccg
     1441 tccggccatt gggagatcgc cggcgcgccg gtacgtttcc aatggggtta tttcccgaaa
     1501 gaagcgcccg ccttcccgcc tggcttgacc gcgacgctca tcgaacaggc ccgcctgccc
     1561 ggcattctcg gcgacaagca cgcatcgggc acggaggtca tcgaggagtt cggcgaagag
     1621 catttgagaa ccttgaagcc gatctgctac acctcggccg attcggtgct gcagatcgcc
     1681 gcgcatgaag aggcgtttgg cctcgatcgg ctttacgatc tttgccgcat cgcgcggcgc
     1741 ctgtgcgatc cgctcaagat cggccgcgtc atcgcgcgcc cgtttgtcgg ccgccgcgcc
     1801 ggcgaatttt cgcgcacgcc gcgacgcaag gatttcgcca ttccgccgcc gcccggcaat
     1861 cttttcgaac gcgcagccaa agccgggcgc gacatcgtct cggtcggcaa gatcggcgat
     1921 atcttcgccc atagccatac cggccgcgag gtgaagggcg cggacaatat ggcgcatgtc
     1981 gatctggcgc ttcaggcgtt ggcgagtgtc gcggatggcg gcctgatttt cgtcaatctg
     2041 gtcgatttcg ataccgaata cggccatcgc cgcgacgtcg ccggctacgc cgcctgcctc
     2101 gaagcgttcg atcggcggct gccggagatc atcgccgcga tgcggccgga cgattacggc
     2161 atcgtcaccg ccgaccacgg caacgatccg acctggcgcg gcttcgacca tacgcgcgag
     2221 catgtcccga tcctcggctt cggcgacggc gtgaagccgg cgccgatcgg ccggcgcaag
     2281 acgctcgccg acatcgcgca aaccttatcg aaaaagctcg gcctgccgcc cggccccaac
     2341 ggcgaaagct ggatttgatc cggtgagcgg tttcgcattg gacccgcgcc ttgccgccga
     2401 tacgctggcg ctcggcgatg cgcgcttgtc gcgcgtcctt ttgatgaacg acgcgcgctt
     2461 tccttggctc atcctcgttc cgcgccgagc cggtctcgcc gagatcgccg agctttcgcc
     2521 gaacgagcgc gagactctca tggaggagat cgtcgcagtc tcgaacgcgc tttcgcagcg
     2581 ccaaggcgtc gagaagatca atgtcggcgc gctcggcaat gtcgtgcggc agttgcacgt
     2641 ccacattgtc ggccgctcca gggacgatgc ggcgtggccc ggaccggtct ggggctccgg
     2701 cgcgcgccag ccctatcaga acgccgcggc gcagcaactt ttgcgcgagc ttgcgcagcc
     2761 gatcgctcat attttaatcg ccgcgtcgga cgaatccggc tgaaagccac ggcttgcgcg
     2821 gcaagacccg cttggatcgc atggcgatcc atgcgccagt tagcgccttg cgcgcctgag
     2881 gggcggcgac tagtgtccgc gccgaacaag ggcgacaagc ccgaccggcc atgtcttcga
     2941 gagcgcgatg acgatcccct cctcttctgt cgattatgtc gtcgaccgcc gacggatgcg
     3001 ccgccaggcg acgttctggc gcgccgcggc gttcgtcatt ctcgttttgg cgatcatcgc
     3061 gctgggctgg cgctatagcg gcctgcccac cggctcgcgt ctcgaatccc atatcgcccg
     3121 cgtctcgatc accggcctga tcaccggcga cgaagcctcg ctgaagctga tccgtgacct
     3181 tcgcgattcc aacgccaagg cggtgctgct ctcgatttcg agccccggcg gcaccacggt
     3241 cggctcggaa aggctttatg aggcgctgcg ccgcctcgcc gcgaagaaac cggtggtcgc
     3301 ggtggtcgac aacatggcgg cgtcaggcgc ctatatcgcg gcgctcggcg cggaccggat
     3361 tttcgccgag ggcaattcgc tggtcggctc gatcggcgtg ctcgtccagt atccgaactt
     3421 ttccggcctg ctcgacaaga tcggcgtgaa atacgaagcg atcaagtcct cgcctttgaa
     3481 agccgcgccg aacggtttcg agccgaccag cgaagaagcg cgcgccgcca tggcttcgct
     3541 tgtctccgac agctacgact ggttcaagaa gctggtgaag gaccgccgcg gcctttccga
     3601 cagcgaattg gccgccgtcg acgacggacg ggtctttacc ggccggcagg gcctttcctt
     3661 gaagctcatc gacgcgatcg gcggcgagcg cgaggcgatc gcctggctcg aaaaggacaa
     3721 gggcgtcgcc aaaaaccttc ccattctcga ttggaagccc cagcgcagcc tcggcggctt
     3781 cggatttttc ggctcggccg cggccgccgc cgattggctc ggcatgacga acctcggcgc
     3841 gcttttgcgg cagagcgagc ttgccggcga aggtcaattg cttgacggtc tcgtgtcgat
     3901 ttggcagctt cctgatcgag actgagtgga cattccgccg cgcgtccctt tgccggccgc
     3961 caagaatggg gaaatccatt gataaagtcc gaactcgtgc agcgcatgtc ggcgcgcaat
     4021 ccgcatctct accagcgcga cgtcgaaaac atcgtcgacg ccattctcga tgagatcacg
     4081 gcggctctgg cgcgcggcga aagggtggag ttgcgcggtt tcggcgcttt ctcgaccaag
     4141 cagcgcgccg cccgcaccgg ccgcaatccg cgcaccggcg accaggtccc ggtgatcgaa
     4201 aaggtcgttc cgttcttcaa gaccggcaag gaaatgcgcg agaggctgaa caagggcgcc
     4261 aacggctgac gcggcttttt cgatgttgag ggtcgcgaaa ttcgttgtcc ttgcgctcgt
     4321 cgccgtcgtc ctgctgctct tcgctttcgc caatcgacaa atcgtcagcg tttccttcga
     4381 tccgttcgcc tcggccgaag cgcccgcctt tgcgcttgcg gcgccgttat tcctggttct
     4441 gatgctgacc ttgatcctcg gcgtgattgt cggcggcgcg gcggtgtggc tgaatcaaag
     4501 ccgattccgc cgggcggcgc ggcaaagccg cgccgagatc gagcgccttc gcgcgcagtc
     4561 cgcaaacgta ccgagcgtcc actgatttcg atcagcgttc ggcaagcgag gcccgccatg
     4621 caggtgatcg acgccaaggc ggtcgacgaa cggctcgatt ttccggctct gatcgacgcc
     4681 ttggccgagg cgttccgcgg cgcctttaag gcgccggcgc gcgtccatca tcagatcgag
     4741 cgcgccggcg ccgccgcgac gcatcttttg atgccggcct ggacgacgag cgcctatcgg
     4801 cagggcgcgt tcctcggcac gaagatcgtc agcgtctttc cggacaatgc cgcgcgcggc
     4861 ttgcccgcgg tgcatggagt ctacgtcctg caatcgggcg agacaggcgc gccgctggc
//
LOCUS       DATGJW010000073         7329 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_532371, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000073 DATGJW010000000
VERSION     DATGJW010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7329)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7329)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7329
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_532371"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..417
                     /locus_tag="VKU03_02200"
     CDS             <1..417
                     /locus_tag="VKU03_02200"
                     /EC_number="2.1.1.63"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003908 -
                     methylated-DNA-[protein]-cysteine S-methyltransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylated-DNA--[protein]-cysteine
                     S-methyltransferase"
                     /protein_id="HLJ70099.1"
                     /translation="ARMARLLRLHYGAVELAHGAAPEATKRALDAYFAGDVGALASLQ
                     TATRGSLFQRGVWAALRTIPPGATTTYGELAAKLGRPNASRAVGLANGANPIAVVVPC
                     HRVIGANGDLTGYGGGLPRKRWLLDHERRYAPPPKA"
     gene            638..1900
                     /locus_tag="VKU03_02205"
     CDS             638..1900
                     /locus_tag="VKU03_02205"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLJ70100.1"
                     /translation="MAKDRFEVVVIGGGAAGIAAARRLNEAGVEALIVEARDRLGGRA
                     WTVMDSAGHALDLGCGWLHSADLNPWREIADRQGVAVDRTPPPWMRPSAQTGFPEAEQ
                     AAFRDALMRFRRRADAAGLAGPDRPASALIEPTDRWTPLLNAVSTFYSGAELDRVSIV
                     DLARYEDTGVNWRLSSGYGALIARYGAGLAAQLGCAVEQIDRRGQSLRIATPHGTIAA
                     RAAIIALPSDIVAAKPDLFLPALPEKTEAASGLPLGLADKLFLSLTEPEQFAKDSRCW
                     GGTDRSAIAAYHLRPFGRPMIEAYFGGALAAELEKGGEAAFFDFAAGELAGLFGADFR
                     RRVRPLAHHSWAADPFARGSYSYALPGKADCRAALAASVENRLFFAGEACSRPDFSTA
                     HGAYRTGIAAADQAIAARRGAKARKRAL"
     gene            complement(1965..3506)
                     /locus_tag="VKU03_02210"
     CDS             complement(1965..3506)
                     /locus_tag="VKU03_02210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006814 - sodium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HLJ70101.1"
                     /translation="MTIFELTLALLLGSVLIAGLARKLAAPYPAFLALAGAGLAFLPH
                     APVVEIEPDLALALFIAPVLLDAAFDTSPRDLKRYAAPIVSLALVAVVLTTAAVAYVG
                     HELVGLPTAAAIALGAIVAPPDAVAAAAVLGQLKPPRRAVAILQSESLLNDAGALLIY
                     RAAVVAATGPLVLATAGPMFLLSAAGSVVFGYVFARFMRAALPYVRDAASTTVVQFVT
                     VFAVWILADKIGLSAIIAVVVFAMTLAQGPPRALAARNRISTYSVWETAVYVLNVLAF
                     VLMGLQARPILSRLADDNRRGAFLLAAASLATAVTVRLVWVLASSAVTRAFRRSQGRP
                     SESDLRSDILIGWCGMRGLVTVAAALALPEGFPGRDPIVLAAFCVVLGTLVIQGLTLR
                     PLLLLLKFKQDNSVEEEVSRGRVAIMDAAIRSLDGDASKAAMVVRKQYAAAREVAENP
                     EAPQGATDFDELRLRAIESQRDELDRLRRQGAIGDEAYHRLQEEIDWAELDAAPVGRF
                     QPLMT"
     gene            complement(3616..5028)
                     /gene="glmU"
                     /locus_tag="VKU03_02215"
     CDS             complement(3616..5028)
                     /gene="glmU"
                     /locus_tag="VKU03_02215"
                     /EC_number="2.7.7.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589750.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003977 - UDP-N-acetylglucosamine
                     diphosphorylase activity [Evidence IEA];
                     GO_function: GO:0019134 - glucosamine-1-phosphate
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0006048 - UDP-N-acetylglucosamine
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional UDP-N-acetylglucosamine
                     diphosphorylase/glucosamine-1-phosphate
                     N-acetyltransferase GlmU"
                     /protein_id="HLJ70102.1"
                     /translation="MPSNKAAQSEAPNVAERAVTPAKERRCLAIVLAAGEGTRMKSAR
                     PKALHPIAGRAMIGHALKAAAEAGADALAVVVGPGREDVAAEAKRCSPEAAIFVQERR
                     LGTAHAVLAARAAIEQGFDDLLVVYADIPLLTAATLRKLRAAIAGGAAVAALGFKARN
                     PAGYGRLVAANGRLLAIREDKDASPEEKAIDLVNAGPMALDGRRALDILSRIGSRNAQ
                     NEFYLTDAVAVAAELGLGAGVEIAMESETMGVNDRAQLAAAEAEMQKRLRERAMAEGA
                     TLIAPETIFLSEDTTIGRDAVIEPHVVIGKGVEIGEGAVIHSFSHLEGAKIGPNASIG
                     PFARLRPGADIGRKAKIGNFVEVKNSLVEEGAKANHLTYLGDARVGPAANIGAGTITC
                     NYDGFDKHFTDIGAEAFIGSNSALVAPVKIGDGAYIGSGSVITEDVEKDALALGRGRQ
                     AAKPGWAARFRAGRKKKERG"
     gene            5270..6802
                     /locus_tag="VKU03_02220"
     CDS             5270..6802
                     /locus_tag="VKU03_02220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004448154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HLJ70103.1"
                     /translation="MHPRRDFLKLAMAGAMSGATSAFGVSLAASAAEPSNAPTPFGPN
                     TVRDLSRALAKKPYEAPKAPLPDPFSSLTYDQYVAIRPKPGSAVWSGDNVGFAIEPLH
                     RGFIFSTPMDIYIVEGGFARKLTYESSAFEFGKLVIPPNLPDIGFSGFRVLRTVAGGS
                     YDVAIYQGASFFRAEARGQNFGVTARGLSIRTADPQGEEFPIFRAVWIEKPTLTDNVL
                     VIHALLDSPSLTGAYRFTLRPGEATIIDTEMTLNTRAAVDHFGLGAMSATYLFGPLDH
                     KRPDDIRPMVHEVDGLQMLTGKGEWVWRPVSNRNTLQISAFTDSNPRGFGLLQRNRSF
                     EAYQDDDAHWELRPSLWIEPIGDWADGEVRLVEIPSDSENNDNLIVYWRPRTSLAANS
                     EIAYAYRQFWCWSPPAKPPLATVVESREGKFGAKLRRFLAEFTSDAFAKPERLAEIKP
                     NIAAKPGRIVSIRTFPSKERMTFRVLFDLEPTSDGVSEIRLVLEAEGKPASETWLYRW
                     TV"
     gene            6790..>7329
                     /locus_tag="VKU03_02225"
     CDS             6790..>7329
                     /locus_tag="VKU03_02225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HLJ70104.1"
                     /translation="MDGLKAETSHRRPAMPPEAPLDMPTQSLTHFARADRRALKAPDL
                     AYTPWLSRLFIFGGALALTGYGANEMYGVVKIGEVTLLEWVLVALFVANFSWIALAFT
                     SSVAGFFWLLFHGGGGGAPVELKCKTAIIMPIYNEAPARVFAAMQAICEDVRATGHGD
                     AFDYFFLSDTTDPDIFIAEE"
BASE COUNT         1209 a         2533 c         2393 g         1194 t
ORIGIN      
        1 gcccgcatgg cgagattgct gcgccttcat tacggcgctg tcgagcttgc ccatggcgcg
       61 gcgccggagg cgacaaagcg cgcgctcgac gcctatttcg ctggcgacgt cggagccttg
      121 gcttccctcc aaacggcgac gcgcggctcg cttttccagc gcggcgtatg ggcggcattg
      181 cgaaccattc cgcccggcgc gacgacgacc tatggcgaac tcgcggcgaa gctggggcgc
      241 cccaacgcga gccgcgcggt cggcctcgcc aatggcgcca atccaatcgc cgtcgtcgtg
      301 ccctgccatc gggtgatcgg cgcgaacggc gacctgaccg gctatggcgg cggcctgccg
      361 cgcaagcgct ggctgctcga ccacgagcgc cgatacgccc cgccgcccaa ggcttaaagc
      421 cggggaataa ccggggcgca tttccgggcg gcattctagg cgcagtcgcg gaccgcgccg
      481 cttccgccgt cgcgtctaaa ggcgaaaccg gacgctgcgc cggccccggt tagcgaccgc
      541 ggctataagc ctgcttggcg aagcccgccc gccaggattg gcggaacaag cggcaaaatg
      601 cgatcttccc gctcgcatag tcgcggaacc gcgaatcatg gccaaggatc gattcgaagt
      661 cgtcgttatc ggcggcggcg cagcgggaat cgcggcggcg cggcggctca atgaggcggg
      721 cgtcgaggcg ctgatcgtcg aggcccgcga ccggcttggc ggccgcgctt ggaccgtcat
      781 ggattcggcc ggacacgcgc tcgatctcgg ctgcggctgg ctgcattccg ccgacctcaa
      841 tccctggcgc gagatcgccg accgccaggg cgtagcggtc gaccgcacgc cgccgccctg
      901 gatgcggcct tccgcccaga ccggtttccc tgaagccgag caggcggcct tccgggacgc
      961 gctaatgcgt tttcggaggc gcgccgacgc ggccgggctc gccgggccgg acaggccggc
     1021 ttcggcgttg atcgagccga cggatcgctg gaccccgctg ctcaacgccg tgagcacgtt
     1081 ctatagcggc gccgagcttg accgcgtctc gatcgtcgat ctcgcccgct acgaggatac
     1141 gggcgtcaat tggcgcctct cgtcgggcta tggcgcgctg atcgcccgct acggcgccgg
     1201 tctcgcggcg cagcttggct gcgcggtcga gcagatcgac cggcgcggcc aaagcttgcg
     1261 gatcgcaacg ccgcacggga ccatcgccgc gcgcgcggcg atcatcgccc tgccaagcga
     1321 catcgtcgcc gccaagcccg atcttttcct gccggctttg ccggagaaaa ccgaggccgc
     1381 ctccggcctg ccgctgggac tcgccgacaa gctgttcctg tcgctcaccg agcccgagca
     1441 attcgccaaa gacagccgct gctggggcgg aacggaccgc agcgccatcg cagcctacca
     1501 cttacggccg ttcggccggc cgatgatcga ggcctatttc ggcggcgcgc tcgccgccga
     1561 gctagaaaag ggcggcgagg cggctttctt cgatttcgcc gccggcgagc ttgccggact
     1621 gttcggcgcc gatttccgcc ggcgcgtccg gccgctcgcc catcattcct gggccgccga
     1681 ccctttcgcg cgcggctcct attcctacgc attgcccggc aaggccgatt gccgcgccgc
     1741 gctcgcagca tccgtcgaga atcgcctgtt cttcgccggc gaggcctgct cgcgcccgga
     1801 tttttcgacg gcgcatggcg cgtaccggac cgggatcgcc gccgccgatc aggcgatcgc
     1861 ggcgcggcgc ggcgcgaaag cgcgaaagcg cgcgctgtag ccgcgctcgg cgagcgcggc
     1921 tgacgcggtc gccgagcgcc tctacaaagc agggatacgt actttcacgt catcagcggc
     1981 tggaatcttc caaccggcgc ggcgtcgagc tcggcccaat cgatctcctc ctgcaggcga
     2041 tgataggcct cgtcgccgat cgcgccttgt cggcgcaggc ggtcgagctc gtccctctgg
     2101 ctctcgatcg cgcgcaggcg caattcgtcg aaatcggtcg cgccttgcgg cgcttcagga
     2161 ttttccgcga cctcccgcgc cgccgcgtat tgcttgcgga ccaccatggc ggcttttgaa
     2221 gcgtcgccgt ccaggctcct gatcgccgcg tccatgatcg cgacgcggcc gcgcgaaacc
     2281 tcctcctcca ccgaattgtc ctgcttgaat ttcagcaaga ggaggagcgg cctcagggtg
     2341 aggccttgaa tgaccaacgt gccgagcacc acgcagaacg ccgcgagcac gatcggatcg
     2401 cggccgggaa atccctccgg cagcgccaac gccgcggcga cggtgaccag gccgcgcatc
     2461 ccgcaccaac cgatcaagat atcgctgcgc agatcgcttt cgcttggtcg gccttgcgag
     2521 cgccggaacg cccgcgtgac ggcgctcgac gccaggaccc aaacgaggcg caccgtgacc
     2581 gcggtcgcca aagaagccgc agcgagaaga aaggcgccgc ggcgattgtc gtcggcgagg
     2641 cgggagagga tcggccgcgc ctgcaagccc atcagcacga aagcgaggac gttgaggaca
     2701 taaaccgcag tctcccagac cgaataggtg ctgatccggt tgcgcgccgc aagggcgcgc
     2761 ggcggacctt gcgccaaggt catcgcgaag acgacgacgg caatgatggc cgaaagcccg
     2821 attttatcgg cgaggatcca gaccgcgaaa accgtcacga attgcacaac cgtcgtgctc
     2881 gccgcatcgc gcacataagg cagcgcggcg cgcatgaacc gggcgaagac gtagccgaag
     2941 acgacgcttc cggcggccga aagcagaaac atcggcccgg ccgtggcgag gacgagcggg
     3001 ccggtcgccg ccacgaccgc ggcgcgatag atgagcagag cgccggcgtc gttgagcagg
     3061 ctctccgatt gcaggatcgc cacggcgcga cgcggcggct tgagctggcc gagcacggcc
     3121 gccgcggcca ccgcgtcggg cggcgcgacg atcgcgccga gcgcgatcgc ggcggcggtc
     3181 ggaaggccga cgagttcatg gccgacatag gcgaccgccg ccgtcgtcag gacgacggcg
     3241 acaagggcga gcgaaacgat cggcgcggcg tagcgcttca gatcgcgcgg cgacgtatcg
     3301 aaagccgcgt cgagcagaac cggcgcgatg aacaaggcca gcgcaaggtc aggctcgatc
     3361 tcgacgaccg gcgcatgcgg cagaaaagcc aagcctgcgc cggccagcgc gagaaaggcc
     3421 ggatatggcg cggcgagctt gcgcgccagg cccgcaatca gcacggagcc gagaaggaga
     3481 gcgagggtga gctcgaaaat ggtcatgagt ttaggcgggc atatcgcaat tggcgctcaa
     3541 cttcgtaccg ccacaggccc ctcccgtacc ctcgcccgcg aagctgggga gggtacggga
     3601 gggggccgcc cgcccctacc cgcgctcctt cttcttgcgc cccgcgcgaa acctcgccgc
     3661 ccatcccggc ttggcggcct gccttccgcg tcccagcgcg agcgcgtctt tttcgacgtc
     3721 ttcggtgatc accgagcccg agccgatata ggcgccgtcg ccgatcttga ccggcgcgac
     3781 cagagccgaa ttcgagccga tgaacgcctc cgcgccgata tcggtgaaat gcttgtcgaa
     3841 gccgtcgtaa ttgcaggtga tcgtccccgc gccgatattg gcggccggac cgacgcgggc
     3901 gtcgccgaga taggtcaggt gattggcctt ggcgccctcc tcgacgagcg agttcttcac
     3961 ctcgacgaaa ttgccgatct tcgccttgcg cccgatatcc gcgcccggcc gcagcctggc
     4021 gaacggcccg atcgaggcgt tggggccgat cttcgcccct tcgagatgcg agaaggaatg
     4081 gatcaccgcg ccttcgccga tctccacgcc tttgccgatc acgacatgcg gctcgatcac
     4141 cgcatcgcgg ccgatcgtgg tgtcctccga aagaaagatc gtctccggcg cgatcagggt
     4201 ggcgccttcg gccatggcgc gctcgcgcag ccgcttctgc atctccgcct cggccgcggc
     4261 gagttgcgcg cgatcattga cgcccattgt ttcgctctcc atcgcaattt cgacgccggc
     4321 gccgagaccg agctcggccg cgaccgcgac cgcgtcggtc agatagaatt cgttctgcgc
     4381 gttgcgcgag ccgatccgcg acaggatgtc gagcgcgcgg cggccgtcca gcgccatcgg
     4441 cccggcattg acgaggtcga tcgccttctc ctcgggactg gcgtccttgt cctcgcggat
     4501 ggcgagcaag cgcccgttcg cggcgaccag ccgcccatag ccggcgggat tacgcgcctt
     4561 gaaaccgagc gccgcgaccg ccgcgccgcc ggcgatggcg gcgcgcagct tgcgcagcgt
     4621 cgcggcggtc agcagcggaa tatccgcata gacgacgagg aggtcgtcga agccctgttc
     4681 gatcgccgcg cgcgccgcga gaacggcgtg cgccgtgccg agccgccgct cctggacgaa
     4741 aatcgccgct tccggcgagc atctcttcgc ctcggcggcg acatcctcgc ggcccggccc
     4801 gacgaccacg gcgagcgcat ccgccccggc ttcggccgcc gctttgagcg cgtggccgat
     4861 catggcgcgg ccggcgatcg gatggagcgc ttttggccgc gccgatttca tccgcgtgcc
     4921 ttcgcctgca gcgaggacga tcgccaggca gcgccgctct ttggccggcg tgacggctcg
     4981 ttcggcgaca ttgggcgctt cgctctgcgc ggctttgttc gacggcatgc ctcgccttaa
     5041 tttgaaaccg cggcgaaagt ccagaaggtc gctagaaaat cggaacgcgg cgctgcaata
     5101 ttaggttata gaaggcgcga gaccctttac ccgccaatgc cgcaaggggc tgcacgtgtc
     5161 ggtaacagct tgttttctcg cgtctttctc aggagcatgg gaggtctttc gacggccgcc
     5221 gcgggcgcgg cggcgaacct gcggcgaacg acaagacgcg tagcggccga tgcatccccg
     5281 ccgcgacttc ctcaaattgg cgatggccgg cgcgatgagc ggagcgacgt cggctttcgg
     5341 cgtttcgctc gccgcgagcg cggccgaacc ttccaatgcg ccgacgccgt ttgggccgaa
     5401 cacggtccgc gacctgtctc gcgcgctggc caaaaagccc tatgaggcgc cgaaagcgcc
     5461 cctgcccgat ccgttcagca gcctgaccta cgatcaatat gtcgcgatac ggccaaagcc
     5521 cggctcggcg gtgtggagcg gcgacaatgt cgggttcgca atcgagcccc tgcatcgcgg
     5581 cttcatcttt tcgacgccga tggacattta catcgtcgag ggcggctttg cgcgaaagct
     5641 cacctacgaa agctcggctt tcgaattcgg caagctggtg atcccgccga acctgcccga
     5701 catcggcttt tccggctttc gcgtcttgcg gaccgtcgcc ggcggctcgt acgacgtcgc
     5761 gatctatcag ggtgcgagct tctttcgcgc cgaagcgcgc ggccagaatt tcggcgtcac
     5821 cgcgcgcggc ctctcgatcc gcacggccga cccgcagggc gaggagtttc cgatctttcg
     5881 cgcggtgtgg atcgaaaagc cgacgctcac cgacaatgtg ctcgtcatcc acgcgctgct
     5941 cgactcgccg agcctgaccg gcgcctatcg cttcaccttg cgccccggcg aggcgacgat
     6001 catcgacacc gagatgacgt tgaacacccg cgccgcggtc gatcatttcg gcctgggcgc
     6061 gatgtccgcc acctatctgt tcggaccgct cgatcacaag cgacccgacg atatccgccc
     6121 gatggtccac gaagtcgacg gtttgcagat gctgaccggc aagggcgaat gggtgtggcg
     6181 gccggtctcg aaccgcaaca ccctgcaaat ctcggctttc acagactcga acccgcgcgg
     6241 cttcggcctc ctgcagcgca atcgctcctt cgaagcctat caggatgacg acgcgcattg
     6301 ggagctgagg ccctcgctct ggatcgagcc gatcggcgat tgggccgacg gcgaggtgcg
     6361 gctcgtcgaa attccttccg actcggaaaa caacgacaat ctcatcgtct attggcggcc
     6421 gcgcacgagc ctggccgcca attccgagat cgcctacgcc tatcggcaat tctggtgctg
     6481 gagccctccc gccaagccgc cgctcgccac agtcgtcgag tcgcgcgagg gcaagttcgg
     6541 cgcgaaattg cgccgcttcc ttgccgaatt taccagcgac gccttcgcca agccggagcg
     6601 cctcgccgaa ataaagccga acatcgccgc caagccgggc cggatcgttt cgatccggac
     6661 cttcccgtcc aaagaacgga tgacttttcg cgtgcttttt gatctagagc caacctcgga
     6721 cggcgtgtcg gaaatccgcc tcgtccttga ggcggaaggc aagcccgcca gcgaaacatg
     6781 gctctatcga tggacggtct aaaagccgag acgagccaca ggcggccggc catgccgccg
     6841 gaagcgccgc tcgatatgcc gacgcaatcg ctgacgcatt tcgcccgcgc cgatcggcgc
     6901 gcgctcaagg cgccggacct tgcctatacg ccgtggcttt cgcgcctgtt cattttcggc
     6961 ggcgcgctgg cgcttaccgg ctacggcgcc aatgaaatgt acggcgtcgt caagatcggc
     7021 gaggtgacgc tgctcgaatg ggtcctggtg gcgctcttcg tcgccaattt ttcctggatc
     7081 gcgctcgcct tcacatcgag cgtggcgggt ttcttctggc tgcttttcca tggcggcggg
     7141 gggggcgccc cggtcgagct gaaatgcaag accgcgatca tcatgccgat ctacaacgag
     7201 gcgccggcgc gcgtcttcgc ggcgatgcag gcgatctgcg aagacgtgcg cgcgaccggc
     7261 catggcgacg ctttcgatta ttttttcctg tccgacacga ccgacccaga cattttcatc
     7321 gccgaggag
//
LOCUS       DATGJW010000074         3368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_947324, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000074 DATGJW010000000
VERSION     DATGJW010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3368
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_947324"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..641
                     /locus_tag="VKU03_02230"
     CDS             <1..641
                     /locus_tag="VKU03_02230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DNA ligase-associated DEXH box helicase"
                     /protein_id="HLJ70105.1"
                     /translation="FGLPIYKWRPQAEIIAEIERWRRANAEDGRASVIAAYPLGKAQR
                     ILRRLDPSLGPIVCHGAVEAMNEVYRSRGIPLPPTVVTRDLADRKALAGAVILAPPSA
                     LSSPWMRRFGDYSDAFASGWMQVRGNRRRRGVDQGFVLSDHADWPGLLRAIEATGARR
                     ILVTHGGVAPLVRYLGEKGYDAAPIHAEYGDETLEREEAKEEAQEATPGGTA"
     gene            638..2392
                     /locus_tag="VKU03_02235"
     CDS             638..2392
                     /locus_tag="VKU03_02235"
                     /EC_number="6.5.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003910 - DNA ligase (ATP) activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HLJ70106.1"
                     /translation="MKDFAALYHRIDAATSTKRKSEALQDYLRAAIADPSRHASAAWA
                     VYFLAGGKPRQMAPTKLLWRLALAETGLPDWLLGESYENVGDLAETLALLLPPPTDSE
                     DLPLDAWMNGRLLALRGMEDEDKFESLRAWTRRLPAAERLPFFKLITGAWRVGVSQRQ
                     VVQALAEATGVPAKAMAQRMMGYTQSGRLLLADDFEALIAAEDDARPQGAASGQPYPF
                     FLAHPFQAPLEDMPATLGPIENWLIEWKFDGIRAQYIHRAGQWWLWSRGEDLISASYP
                     DLAPLANFAPSGAVLDGELVVVGTDPAEGARDRAVDAIDDIRPFASLQQRLGRKALTA
                     KILKELPVALIAYDLLELDGIDIRQRPQRERRALLERVVADAFRRAAERGQDIPLRLS
                     PALHRPDWASLATLREQARGLGAEGMMLKGMSSAYGVGRRKSGPAGEDLWWKWKLNPM
                     SVDAVLIYAQRGSGRRSGVYSDYTFAVWSGPPEDSSRTLVPFAKAYSGLTDEEMRQVD
                     ANIRRTTIETFGPVRSVKPIMVFELGFEGIAPSKRHKSGVATRFPRMLRWRQDKPAEE
                     ADTLEQLRALLPKEARGG"
     gene            2550..2846
                     /locus_tag="VKU03_02240"
     CDS             2550..2846
                     /locus_tag="VKU03_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase domain-containing
                     protein"
                     /protein_id="HLJ70107.1"
                     /translation="MKRDEIILTLKEREADLRARGVRHAALFGSVARNEQRPDSDIDI
                     LVDLDPAIIATMFDYAGVKDYVAGLFQDSADVIDRDALKPRFRARAAADAIYAF"
     gene            2836..3177
                     /locus_tag="VKU03_02245"
     CDS             2836..3177
                     /locus_tag="VKU03_02245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HepT-like ribonuclease domain-containing
                     protein"
                     /protein_id="HLJ70108.1"
                     /translation="MLSEIDRDALEDIRDNIARVSRFVDGFDLDGFIADDKTFYAATR
                     CLEIISEASRRLSPAFKARFPEIPWKDVAGSGSIYRHSYENVQERRVWATIHEALPPL
                     RAVAEAALRDG"
BASE COUNT          593 a         1120 c         1089 g          566 t
ORIGIN      
        1 ccttcggcct gccgatctat aagtggcgcc cgcaggccga gatcatcgcc gaaatcgagc
       61 gctggcgccg cgccaacgcc gaggacggac gggcgagcgt gatcgcggcg tacccgctcg
      121 gcaaggcgca gcgcatcctg cgccggctcg atccgtcgct cggcccgatc gtctgtcacg
      181 gcgcggtcga ggcgatgaac gaggtctacc gcagccgagg aattccgctc ccgccgacgg
      241 tcgtcacgcg cgacctcgcc gatcgcaagg cgctcgccgg cgccgtcatt ctcgcgccgc
      301 ccagcgcctt gtcgagccca tggatgcgcc ggttcggcga ttattccgac gccttcgcca
      361 gcggctggat gcaagtgcgc ggcaatcgcc gacggcgcgg cgtcgaccag ggcttcgtcc
      421 tttccgacca tgccgactgg ccgggcctgc ttcgagcgat cgaggcgacc ggcgcgcggc
      481 gcatcctcgt cactcatggc ggcgtcgcgc cgctcgtccg ctatctcggc gagaagggtt
      541 acgacgccgc gccgatccac gccgaatatg gcgacgagac gctcgagcgc gaggaagcga
      601 aggaggaggc gcaagaggcg acgccgggcg gtacggcatg aaagacttcg ccgccctata
      661 ccatcgcatt gacgccgcga cctcgaccaa gcgcaagagc gaagcgctgc aggactacct
      721 gcgcgccgcc atcgccgatc cgtcgcgtca cgccagcgcc gcctgggcgg tctatttcct
      781 cgccggcggc aagccgcggc agatggcgcc gaccaagctg ctgtggcggc tggcgctggc
      841 ggagaccggc ctgcccgact ggctgctcgg cgaaagctac gagaatgtcg gcgatctcgc
      901 cgagactctg gcgctgcttc tgccgccccc gaccgacagc gaagacctgc cgctcgacgc
      961 ctggatgaac ggccgcctgc tggcgctgcg cggcatggag gacgaggaca agttcgaatc
     1021 cctccgcgcc tggacccgcc ggctgcccgc cgccgagcgc ctgccctttt tcaagctgat
     1081 caccggcgcc tggcgggtcg gcgtctcgca gcggcaagtc gtccaggctc tggccgaggc
     1141 gaccggcgtt ccggccaagg ccatggcgca gcggatgatg ggctataccc aatcgggccg
     1201 gctgctcctc gccgacgatt tcgaggcgtt gatcgcggcc gaggacgacg ccaggccgca
     1261 aggcgcggcg agcggccagc cctatccgtt cttcctcgcc catccctttc aggcgccgct
     1321 cgaagacatg ccggcgacgc tcggtccgat cgagaactgg ctgatcgaat ggaagttcga
     1381 cggcatccgc gcgcaatata tccaccgcgc cgggcaatgg tggctgtggt cgcgcggcga
     1441 ggatctgatc agcgcgagct atcccgacct cgcgccgctc gcgaatttcg cgccgagcgg
     1501 cgccgtgctc gacggcgagc tggtcgtggt cggaacggac ccggccgaag gcgcgcggga
     1561 ccgcgccgtc gacgcaatcg acgacatcag gcctttcgcc tcgctgcagc agcggctcgg
     1621 ccgcaaggcg ctcaccgcga aaatcctgaa agagctgccc gtcgcgctga tcgcctatga
     1681 tcttctcgaa ctcgacggga tcgacatccg ccagcggccg cagcgcgagc ggcgggcgct
     1741 gctggagcgc gtcgtcgccg acgcctttcg ccgcgccgcc gaacgcggcc aggacattcc
     1801 gctccgcttg agccccgcct tgcatcggcc cgattgggcg agcctcgcga ctttacgcga
     1861 acaggcgcgc ggcctcggcg ccgagggcat gatgctcaag ggcatgagca gcgcctatgg
     1921 ggtcggccgg cgcaagagcg gccccgccgg cgaggattta tggtggaaat ggaagctcaa
     1981 cccgatgagc gtcgacgcgg tgctgatcta cgcgcagcgc ggcagcggcc gacgctccgg
     2041 cgtctatagc gactatacgt tcgccgtctg gagcggcccg ccggaagatt cttcgcgcac
     2101 gctcgtgccg ttcgccaagg cctattcagg cctcaccgac gaggagatgc gccaggtcga
     2161 cgccaatatc cggcgaacga cgatcgagac cttcggcccg gtgcgcagcg tcaagccgat
     2221 catggtcttc gagctcggct tcgaaggcat cgcgccgagc aagcggcaca aaagcggcgt
     2281 cgcgacgcgc ttcccgcgca tgctgagatg gcgccaggac aagccggcgg aggaagcgga
     2341 tacgctagag caattgcggg cgctgctgcc gaaggaggcg cggggcgggt gaggttggcg
     2401 ggcttcgtat tttcgctttt caaccggcat tgaacgccta ccaagccgtg gtagaataat
     2461 tgtggcggaa agccagatat tcctcaaaat ggagagcgct cgcgccctga cggcgcgaat
     2521 cgcacaatcg acagtttgac cgagacgaca tgaagcgcga cgagatcatc ctgaccttga
     2581 aggaacgcga agccgacttg cgcgcgcgcg gcgttaggca tgccgcgttg ttcggttcag
     2641 tcgcgcgcaa cgagcagcgc cccgacagcg acattgacat tttggttgac ctcgatccgg
     2701 cgattatcgc cacgatgttc gattacgccg gcgtgaaaga ctatgttgcg ggcctgtttc
     2761 aagattccgc ggacgtgatc gaccgagacg ccttgaaacc ccgctttcgc gcgcgtgcgg
     2821 cggctgacgc catttatgct ttctgaaatc gaccgcgacg ctcttgagga tattcgcgac
     2881 aacatcgccc gcgtgagccg tttcgtcgac ggttttgacc tcgacggctt catcgctgac
     2941 gacaagacgt tttacgcggc gacgcgatgc ctagaaatca tctctgaagc ttcccgccgt
     3001 ctgtctcccg ccttcaaggc gcgttttcct gaaattccgt ggaaggacgt agccggctca
     3061 ggcagcatct accgccacag ctacgagaac gttcaagaac gtcgcgtttg ggcgacaatt
     3121 cacgaagcct tgcccccact tcgcgcggtg gcggaggcag cattacgcga cggatagctt
     3181 tgctcaaaaa tgcagccggc tcggcatgaa cgcggcggtc tcgtcgtgac agttggccgc
     3241 ttgtatcggc caattgaatg cactgtcacc gtaacgcccc aattgaatgc actgtcaccg
     3301 taacgcccaa agcattcgca aatattttct tggcgagctg aaaaattttc ctttgttttc
     3361 aatggctc
//
LOCUS       DATGJW010000075         3743 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_221238, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000075 DATGJW010000000
VERSION     DATGJW010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3743)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3743)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3743
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_221238"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..625)
                     /locus_tag="VKU03_02250"
     CDS             complement(155..625)
                     /locus_tag="VKU03_02250"
                     /EC_number="3.5.4.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0052717 - tRNA-specific adenosine-34
                     deaminase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside deaminase"
                     /protein_id="HLJ70109.1"
                     /translation="MDELAGGRAARPDPMGAAFDAARAAGAAGEVPVGAAIARGGAVI
                     VSAGNRTVLDRDPTAHAEMLALRAAAAALGSERLADCDLYVTLEPCAMCAAAISFARI
                     RRLYYAASDEKGGAVEHGPRFFSQPTCHHAPEVYGGIRASEAAELLRAFFAARR"
     gene            complement(645..1607)
                     /locus_tag="VKU03_02255"
     CDS             complement(645..1607)
                     /locus_tag="VKU03_02255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ70110.1"
                     /translation="MRQGLALFVLAAMAAASAASAQETSFVLPPQAGGPPAPAGPQPD
                     YAFAAYQRGYYITAFEEAMKALSRNPKDAAAMTLIGELYRTGAGIKPNGAEAERWYRL
                     AADLGDRQARFALGLLLLTGQNGAAKDRAVAKALFEQAAAQGHAGALYNLGVMALEGD
                     EKTKPDFAKAADDFKRAADLGDGDAAYSYAVLLRSGRGAPLDTAEAARWMKHAADAGV
                     VAGEVEYAIMLFNGEGVDRDEAAAAKLFGKAAAQNNPIAENRLAHLYATGRGVKLDLA
                     QAAAWNMLARAGGIKDAELDKLAEDLTPQQRAAALETVRRQKMF"
     gene            complement(1607..2248)
                     /locus_tag="VKU03_02260"
     CDS             complement(1607..2248)
                     /locus_tag="VKU03_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HLJ70111.1"
                     /translation="MTDIEKSRLYLLTPPLAETDLADFAALLGDVLSAGDVACVLARI
                     AQGAEGDAKTIVSRLAEIASRAEAALLADNNARLAARAGADGAHIHGPGEALDEALKS
                     LKPERIVGAGLLRSRDDAMRAGEKGVDYVMFGEPRADGFIPPAEQTLERVGWWAEIFE
                     PPCVGFAARLGDIAPLAAAGADFIALADAIWRADRPADALAQAQAALLAPQVN"
     gene            complement(2348..2887)
                     /locus_tag="VKU03_02265"
     CDS             complement(2348..2887)
                     /locus_tag="VKU03_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1465 family protein"
                     /protein_id="HLJ70112.1"
                     /translation="MTRFFDPQTISVVRPGTVSFIQKLAGSNAFKAMFKEGMALVEEA
                     AAYLDGPGREESRQLRRPAALAYATESMRLTTRLMQIASWLLLQRAVNEGEMTLAQAA
                     TDKHRVRLSRQDIACAAEIFEELPDRLRDLSLKSLRLQARVMHLDRCLFARDDAMTIS
                     PSPIASQVERLRAAFQTAM"
     gene            complement(3315..3518)
                     /locus_tag="VKU03_02270"
     CDS             complement(3315..3518)
                     /locus_tag="VKU03_02270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429919.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1192 domain-containing protein"
                     /protein_id="HLJ70113.1"
                     /translation="MAGFDDDLLGAPRKAPTAHEIGQTIDTLSVHELEERIALLKAEI
                     VRLEAAIKARQATKDAASAFFKR"
BASE COUNT          621 a         1221 c         1303 g          598 t
ORIGIN      
        1 cgaagcgagg gcggttacgg gcgcgctgat tccggtgagc ggcggggtgt agtttcgtcg
       61 ttgcgagcga agcgaagcaa tccacttgta ggtacacggc tatgaatgga ttgcttcgtc
      121 gcttcgctcc tcgcaatgac gcctcgtctt gtccctatcg gcgcgcggcg aagaaagcgc
      181 gcagcagctc cgccgcttcg ctcgctctga tgccgccata gacttccggc gcgtgatgac
      241 aggtcggctg agagaaaaag cgcggtccgt gctcgaccgc gccgcccttt tcgtcgctcg
      301 cggcatagta caagcgccgg atgcgggcga aagagatcgc ggcggcgcac atcgcgcaag
      361 gctcgagcgt cacatagagg tcgcaatccg cgaggcgctc cgaaccgagc gccgcggccg
      421 cggcgcgcag cgccagcatc tcggcatgag cggtcggatc gcgatcgagg accgtgcggt
      481 tgccggcgct cacgatgacg gcgccgccgc gggcgatcgc cgcgccgacc ggaacctcgc
      541 ccgcggcgcc ggccgcgcgc gccgcgtcga acgcagcgcc catcgggtcc ggcctggcgg
      601 cgcggcctcc cgccaattcg tccatctttg tcggccttca gggctcagaa catcttctgc
      661 cggcgcaccg tctccaaagc cgcggcgcgc tgctgcggcg tcaagtcctc ggcgagctta
      721 tcgagctcgg cgtctttgat cccgcccgcc cgcgccagca tgttccaggc ggccgcttgc
      781 gcgagatcga gcttcacccc gcggcccgtc gcatagagat gggcgaggcg gttctcggcg
      841 atcggattgt tctgcgcggc ggctttgccg aacaatttgg ccgccgccgc ctcgtcgcga
      901 tcgacgccct cgccgttgaa cagcatgatc gcgtattcga cttcgccggc gacgacgccg
      961 gcgtcggcgg cgtgcttcat ccagcgcgcc gcttcggccg tgtcgagcgg cgcgccgcgg
     1021 ccgctgcgca gcagcacggc gtaagaatag gcggcatcgc cgtcgccgag atcggcggcg
     1081 cgcttgaagt catcggcggc cttggcgaaa tcgggcttgg ttttttcgtc gccctcgagc
     1141 gccatgacgc cgagattgta aagcgcgccg gcatggccct gcgccgcggc ctgctcgaac
     1201 agcgccttgg cgacggcgcg gtccttggcc gcgccgtttt gaccggtgag cagcaggagg
     1261 ccgagcgcga aacgggcttg ccggtcgccg agatcggcgg cgaggcggta ccagcgctcg
     1321 gcttcggcgc cgttcggctt gatcccggcg ccggtgcgat agagctcccc gatcagagtc
     1381 atcgccgcgg cgtctttggg attgcgggag agcgctttca tcgcctcctc gaaggcggtg
     1441 atgtaatagc cgcgctgata ggcggcgaag gcgtaatccg gctgggggcc ggccggcgcc
     1501 ggcggcccgc cggcctgggg cggcaggacg aagctggtct cctgggccga agcggcggag
     1561 gccgccgcca tggcggcgag gacgaacaac gcaagtccct ggcgcatcaa ttgacctgag
     1621 gcgcgagcaa agcggcctga gcctgggcga gcgcgtcggc cggccggtcg gcgcgccaga
     1681 tcgcgtcggc gagggcgatg aaatcggccc cggcggccgc gagcggcgcg atgtcgccga
     1741 gccgggcggc gaagccgacg cagggcggct cgaaaatctc cgcccaccag ccgacccgct
     1801 ccagcgtctg ctcggctggc ggaatgaacc cgtcggcgcg cggctcgccg aacatcacgt
     1861 agtcgacgcc cttctcgccg gcgcgcatcg catcgtcgcg cgagcgcaat aagccggcgc
     1921 cgacgatgcg ctccggcttg aggctcttaa gcgcctcgtc aagcgcctcg cccggtccat
     1981 ggatgtgcgc cccgtcggcc ccggcgcgcg cggcgaggcg tgcgttattg tcggcgagca
     2041 gcgcagcttc ggcgcggctg gcgatctcgg cgaggcggct gacgattgtc ttggcgtcgc
     2101 cctcggcgcc ctgcgcaatg cgggcgagga cgcacgcaac gtcgccggcc gaaagaacgt
     2161 cgccgagcaa ggccgcgaaa tcggccaggt ccgtctccgc cagaggcggc gtcagaagat
     2221 aaagccggct tttctcgata tccgtcatgg cgcccagatc ggcggtcgct ccttgtaggc
     2281 gaccaatggg gccaaaacgt gagacgcgca agggccgccc ggcgcagcga ggccgggcgc
     2341 ttgtcgctta catcgccgtc tgaaacgcgg cgcgcaggcg ctcgacctgg ctggcgatcg
     2401 ggctcgggct gatcgtcatg gcgtcgtcgc gggcgaacag gcagcggtcg agatgcatca
     2461 cccgcgcctg taggcgcagc gacttgaggc tgaggtcgcg caagcgatcg ggcagctctt
     2521 cgaaaatctc cgcggcgcag gcgatgtcct ggcgcgacaa acggacgcga tgtttgtcgg
     2581 tcgcggcctg ggcgagggtc atttcgcctt cattgaccgc acgctgaagc aacagccacg
     2641 acgcgatctg catcaatcgg gtcgtcaggc gcatgctctc ggtagcgtaa gccagggcgg
     2701 cgggccggcg caattggcgc gattcctcgc ggcccggccc gtccaaataa gccgcggctt
     2761 cctcgaccag ggccatgcct tccttgaaca tcgctttgaa ggcgttggag ccggcgagct
     2821 tctgaatgaa ggacaccgtg ccgggcctca cgacggagat tgtctgaggg tcaaaaaagc
     2881 gcgtcatcgc tgaactctcg aagttctcgc attcgtcgcg gcgctttcgg cgccgatctt
     2941 taaaggagcg aacgcaaagt tagcgccgtc gcgcctgcgc cgcttgttta ctctttgata
     3001 aggttaacaa aacgtatagg acaaacaaca aatcaaaaag aaagccgccc gagggcggct
     3061 tgggagttaa aaacgggagg cgtcgaacag agtggacagg agccactcga agatcctgaa
     3121 gaacaggatg aaatctttca taaagcgtaa ttgttaattt tgtcctaacg gacaacaaag
     3181 cctttataga aaaatttttc ttacgctccg tttggggacg gccggcggct cgaaataaga
     3241 cggccgcgcg ctggcgcggc ggcgatccgc ctctcgcgtt ggaaatgaag acagggggag
     3301 ggggcggcgg cccgctaacg cttgaagaag gcgctcgccg cgtccttggt cgcttgtcgc
     3361 gccttgatcg ccgcttcgag ccggacaatt tccgctttga gcagcgcgat gcgctcttcg
     3421 agctcgtgca cggagagcgt gtcgatcgtc tggccgatct catgcgctgt cggcgctttg
     3481 cgcggcgcgc ccaggagatc gtcgtcgaat cccgccatga aaatctccgc cccgatccga
     3541 ggccgccgcc aattaagcgc cggacggttg cgaactcaag gcggcgtcgc ggcgctcagc
     3601 ctcgagccaa gcgcaaggcc caggcggcgg cgaagggcgc gccggcgacc gcggcgaggg
     3661 tcaaggcgca gagcgccagg aagggcggcg cgaacggcgc cgcgccgccg ctcgccgccg
     3721 aggcggcggc gacgccgaaa atc
//
LOCUS       DATGJW010000076         3622 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_325277, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000076 DATGJW010000000
VERSION     DATGJW010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3622)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3622)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3622
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_325277"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..940)
                     /locus_tag="VKU03_02275"
     CDS             complement(<1..940)
                     /locus_tag="VKU03_02275"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLJ70114.1"
                     /translation="MTGGAIETGFRARLAAFDWRALDLRRIAEPIAVCSPLALIVSAQ
                     EPVMIVVGLLFVAHSWRAGDWAWLKSGWLRALLALWVYAFLRTLLIHPTATGVLTALQ
                     WIHFAVYAAALATWILPRKAARDRLLWALAATIAFFSLDCFLQYATGYDIIGRPKEPN
                     RLTSVFRKPGVGAEIGWLFLPAVAGLWGESRRLLALALGVIALAAVLLSGDRMALLIA
                     LASIVLFCLFAARANKALLLAPAAIALVLAGLLYFNPAIYHRQVETTETIVSRVGDSV
                     YGIIWKTGLDIARDHPAFGVGVRNYQAACLDDRYGPL"
     gene            1100..2185
                     /locus_tag="VKU03_02280"
     CDS             1100..2185
                     /locus_tag="VKU03_02280"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLJ70115.1"
                     /translation="MFAIVTQCFGPDLGGVEALMTGLADALSAAGEDIAVFADRAHLA
                     DAFARPYPIERFGFVRPFRRLMKRRAIARAEGLAGIFADSWKSVEAIPPVKAPIAVLA
                     HGMELPPDASRRKARRIAAAFGRARAIVANSAYTADLARRFVAAGGPAILVAPPPAPP
                     QSEPTARALADIDALIAGRGPVLATVARLEPRKGIDATLRALPAILAAFPRAVYLVGG
                     AGADLGRLRRLAASLGVAEAAHFLGRIDEAHKAALLARADLFAMPTRREGASVEGYGI
                     AYIEAAWRGKPALAGRAGGAADAVIDGETGLLCDGADDREVTRALLRLLGDEKLRKRL
                     GDAALARARAEHSWPAALPRYLAAVGR"
     gene            complement(2189..3115)
                     /locus_tag="VKU03_02285"
     CDS             complement(2189..3115)
                     /locus_tag="VKU03_02285"
                     /inference="COORDINATES: protein motif:HMM:NF025736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLJ70116.1"
                     /translation="MSVFDFPSRAAWAALVLCAAAAFCGLAALGVAIRPLSLALPLAA
                     TALCLAASTFYRRYRPDRRLAAMTAGAGFLLAFSAAGGALTYPAAAIGGPLQDKAFIA
                     FERALGVDWPALAAAMSANPYLNLVWALAYMSSLPMLAVVVVALTLTGREERLANFLS
                     LFVVTLLTTVAGSVAGPALGPIPSYGISGEVFSRLGGGGKDFLADFQALRAHQFAVFD
                     LVRLQGIITFPSFHCVLAVLTAWALAPIRWLGRFAIGLSVLVVLSTIPQGGHYVADIC
                     AGLLIGFCAIAVAAGLRLADRPAAAPALAPAE"
     gene            complement(3240..>3622)
                     /locus_tag="VKU03_02290"
     CDS             complement(3240..>3622)
                     /locus_tag="VKU03_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158589.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HLJ70117.1"
                     /translation="TIKAFQYDIACNGYELASGGIRNHRPEAMVKAFEIAGYGEETVK
                     ERFGGMYRAFHYGAPPHGGMAAGVDRIVMLLAGEQNLREVALFPMNQRAEDLLMGAPS
                     PATPKQLRELHLRLNLPEGKTGGG"
BASE COUNT          626 a         1244 c         1249 g          503 t
ORIGIN      
        1 gcagcggccc gtagcgatcg tcgaggcaag cggcttgata gttgcgaacg ccgacgccga
       61 acgccggatg gtcgcgggcg atgtcgagcc ccgtcttcca gatgatcccg tagacggagt
      121 ctccgacccg gctgacgatc gtctcggtcg tctcgacctg gcgatgataa atcgccggat
      181 tgaaatagag cagcccggcg agaaccagcg cgatcgccgc cggcgcaagc agcagcgcct
      241 tgttggcgcg cgcggcgaag aggcagaaga gaacgatcga cgccaaagcg atcagcaagg
      301 ccatgcggtc gccgctcagc agcacggcgg cgagcgcgat cacgcctaaa gccagcgcca
      361 agagccgccg gctttcgccc cagaggccgg ccacggcagg caggaacagc cagccgatct
      421 cggcgccgac gccgggcttg cggaacacgc tggtcagccg gttcggctct ttcggccggc
      481 cgataatgtc gtagccggtc gcgtattgga ggaagcaatc gagcgagaag aacgcgattg
      541 tcgcggccaa ggcccagagc agccggtcgc gcgccgcctt tcgcggcaga atccaggtgg
      601 cgagcgcggc ggcgtagacg gcgaaatgaa tccattgcag cgccgtgagc acgccggtcg
      661 cggtcggatg gatgagaagc gtgcgcaaga aggcgtagac ccagagcgcg agcaaggctc
      721 tgagccagcc gctcttgagc caggcccaat cgcccgcccg ccagctatgg gcgacgaaga
      781 gcagcccgac gacgatcatc accggctctt gcgcgctgac gatcagggca agaggagagc
      841 aaaccgcgat cggctcggcg atgcggcgca gatcgagggc gcgccagtcg aacgccgcaa
      901 ggcgggcgcg aaagccggtc tcgatcgcgc cgccggtcat cttgctcctc ttgaacgatt
      961 tcgcccgcct cccggagggg cgcggcggct ctatcgcaga tgaggcggct gagttcaatt
     1021 ctcgtggcgc ggccggaggg aagcggagaa ttcggatttt ctgctatgga ggccaagcag
     1081 cgtttgagcc ggattcctct tgttcgccat cgtcacgcaa tgtttcggcc ccgatctcgg
     1141 cggcgtggag gccttgatga cgggcctcgc cgacgccttg agcgcggccg gcgaggacat
     1201 cgcggttttc gccgatcgcg cccatctggc cgacgctttc gcgcggccct atccgatcga
     1261 gcgtttcgga ttcgtccgcc cttttcgccg gctgatgaag cggcgggcga tcgcccgcgc
     1321 cgaggggctt gccggaatct tcgctgattc ctggaagagc gtcgaggcga tcccgccggt
     1381 caaagcgccg atcgccgtgc tcgcccatgg catggagctg ccgcccgacg cgtcgcgccg
     1441 caaggcgcgg cgcatcgccg ccgctttcgg ccgggcgcgg gcgatcgtcg ctaattccgc
     1501 ttacacggcc gatctggcgc gccgtttcgt cgccgcgggc ggcccggcga tcctcgtcgc
     1561 gccgccgccg gcgccgccgc agagcgagcc gaccgcccgg gccctcgccg atatcgacgc
     1621 gctgatcgcc ggccgcggcc cggttctggc gacggtcgcc cggctcgagc cgcgtaaagg
     1681 gatcgacgcc accctgcggg ccttgccggc gatcctggcg gcctttccgc gggcggtcta
     1741 tctggtcggc ggcgccggcg ccgaccttgg ccggctgcgc cgcctcgccg cctcgctggg
     1801 cgtcgcggag gccgcgcatt ttctcggccg gatcgacgag gcgcacaaag ccgccctgct
     1861 cgcccgcgcc gatcttttcg ccatgccgac caggcgcgag ggcgcctcgg tcgaaggcta
     1921 cggcatcgcc tatatcgagg ccgcctggcg cggcaagccg gctttggccg gccgcgccgg
     1981 cggcgcggcc gacgcggtga tcgacggcga gacgggcctg ctctgcgacg gggccgacga
     2041 ccgggaggtg acgcgcgcgc tgcttcgcct gcttggcgac gagaagctgc gcaagcggct
     2101 cggcgacgcc gcgctggcgc gcgcccgcgc cgagcattcc tggcccgccg ccctgccccg
     2161 ctacctggcc gcggtcgggc ggtagctttt attcggccgg agccaaagcc ggcgcagccg
     2221 ccggccggtc cgcgaggcgc aagccggccg cgacggcgat cgcgcagaac ccgatcagca
     2281 gcccggcgca gatatcggcg acgtagtgcc cgccttgcgg gatggtcgaa aggacgacca
     2341 gcacgctcag gccgatcgcg aaacggccga gccagcggat aggcgcgagc gcccacgcgg
     2401 tcaggaccgc gaggacgcaa tgaaatgagg gaaacgtgat gatcccctgc aaacgcacca
     2461 agtcgaacac tgcgaattgg tgggcgcgca gcgcctggaa atcggccagg aagtccttgc
     2521 cgccgccgcc gagccgcgaa aagacttcgc cgctgattcc gtagctcgga atcggaccga
     2581 gcgccggccc cgctaccgac ccagcgacag tcgtcaggag cgtgacgacg aacagtgaca
     2641 ggaagttcgc caagcgctcc tcgcggccgg tcaaagtcag cgccacgacg acgaccgcca
     2701 gcatcggcag cgaactcata taggccagag cccaaacgag attgagataa gggttggcgc
     2761 tcatcgccgc cgcgagcgcc ggccaatcga cgccgagcgc acgctcgaag gcgataaagg
     2821 ccttgtcttg aagcggaccg ccgatcgctg cggcgggata agtgagcgcg ccgcccgcgg
     2881 cgctgaacgc caggaggaag cccgcccctg cggtcatcgc cgcaagccgg cgatcggggc
     2941 gatagcggcg gtagaaggtc gaggccgcca gacataacgc ggtcgccgcc agcggcaggg
     3001 ccagcgatag aggtcttatc gcgacgccca gcgcggcgag gccgcaaaat gcggcggccg
     3061 cgcaaaggac gagcgccgcc catgcggcgc gcgaagggaa atcaaagacg ctcatgagac
     3121 ggtccggcca aagatcggac caacctagcc tcgccaagtt aatcaagcct tggcgcaaat
     3181 gcgggggatt cgccgctcca ccgtcttccg gccgagaaag cgcgcctgcc gagagcgccc
     3241 tatccgccgc ccgtcttccc ctccggcaga ttgagccgca gatgcagctc gcgcaattgc
     3301 ttcggcgtcg ccggcgacgg cgcgcccatc agcaaatcct cggcgcgctg gttcatcggg
     3361 aacagcgcga cctcgcgcaa attctgctcg ccggcgagca gcatgacgat gcggtcgacg
     3421 cccgccgcca tgccgccatg cggcggcgcg ccgtaatgga aggcgcggta catgccgccg
     3481 aaccgctcct tcaccgtctc ctcgccatag ccggcgatct cgaaagcttt caccattgct
     3541 tccggccgat ggttacggat accgccagag gcgagttcgt agccattgca ggcgatatcg
     3601 tattggaagg ccttgatcgt ca
//
LOCUS       DATGJW010000077        12076 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1154234, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000077 DATGJW010000000
VERSION     DATGJW010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12076)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12076)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12076
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1154234"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(54..1112)
                     /locus_tag="VKU03_02295"
     CDS             complement(54..1112)
                     /locus_tag="VKU03_02295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="questin oxidase family protein"
                     /protein_id="HLJ70118.1"
                     /translation="MTAALRSAVGDFTREETELERLLDEGRKFSAEFPLFLANHLPMV
                     LVAMRRLGGSDRRLAEYFATYREINRLCPPPPPVAPIAREAWTEALGAREREADYRAF
                     FQEEVARLGARAAAGLYLPRLLPGLAASATHGLMRLAYGAMRNDEAEIAAALGYWAAT
                     YLELGRASGAPPITDDPVEVLVRMQPIETFRHVETELDLLWHFMRAIAQKPEFAPIVD
                     WLAIGPGTFQRVRKASLALYAGTMDFCALHALTGCHWLRLIAPITPDPDLALRYFWQS
                     IAALYPKIGFPGLPDTETLEAWRRAPCPDWEEIAAAAVKSDDEHDLSLVFSAREEGRL
                     YGDRLYQVVAARRMKLIA"
     gene            1341..2612
                     /locus_tag="VKU03_02300"
     CDS             1341..2612
                     /locus_tag="VKU03_02300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007814703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ70119.1"
                     /translation="MKARRILGIAAAISLASLAQAKAADKPYDGVTLTLASQNDQFAT
                     VMADLAPKFKEATGVTVKVDILNYPELLSKVTADFIGHTKGYDLATMDIVWAGQFAEG
                     GYTVELSDWIKRDAAEIHPDDIYPVLMSALGNFKGKQVAFPFAGYANVLAYRTDLYEA
                     AGLKPPQTMEDMVANAYKLTDPPKHYGFVANGQKGPAVAQDWMQYNNEMGGSILGPDG
                     KPALNSAANIKSLTVYKELFDKCAPPGAADFDWGGREEAFRQGLAAQMQTWSIGAASY
                     SDPEKSKIVGKVAITVAPTGKGVPKKYGVGGWGMSINADIDQKHKEAAWLWIKWLTSP
                     AIHKQLNLMGAGSYLRISETHDPELVAKFPFLPVIDEVFKHGDGDYRPRIPQYPQIQD
                     LLGTAVNAVLVGHEDPKKALDEAQAEAVKLF"
     gene            2714..3607
                     /locus_tag="VKU03_02305"
     CDS             2714..3607
                     /locus_tag="VKU03_02305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020572137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLJ70120.1"
                     /translation="MTYPRRRQIFLVLLGAPALIYVLLVAIWPLARGIGYSLYDYNLL
                     RPARTAFVGFGNYSALWANDAARNAIETTAMFTIAAVAVEFILGLGLALLLWRDSAFQ
                     RVCLTLLLIPVTVTPIAVGLIFRALLAPDYGLIGYYLAAWGLSDPHGLLGDPASALWT
                     LVAVDAWQWTPFMALILLAGLKALPTELLEAAEVDGATALQRLRLIILPMLLPAAFLA
                     LVLRTMDAFRIFDIIYSTTNGGPADATNVLMVYAVKQGLEFFNIGFASAIANLMIVCI
                     AIFSAAFIVLVRRADKRANQS"
     gene            3604..4428
                     /locus_tag="VKU03_02310"
     CDS             3604..4428
                     /locus_tag="VKU03_02310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015918088.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLJ70121.1"
                     /translation="MSRSPSASAVIAERSALVVIVAIFLAPVAWMISTAYKPPRDIFS
                     IPPTLSFTPTWANFANVFRYFDLFHLIESSLAISLGAAALSLLIGAPAGYALARAQTK
                     WAIGLAYFFLAVRTVPPVATLLPFYLMMRDIGLLGTWGAVILIDATLNTAFVVWMMFS
                     YFRALPKEMEEAALTDGCSLLGSFLRISLPLVKPGLVASALLCVIFAWNDFLYSSFLT
                     RAESKPISVALLTAYGTKDISWGTLGALAHFSTIPIVAIALVLNRHFVQGLTRGIH"
     gene            complement(4422..5675)
                     /locus_tag="VKU03_02315"
     CDS             complement(4422..5675)
                     /locus_tag="VKU03_02315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70122.1"
                     /translation="MTDLILEKTDAVDQEQGAPGWMTFLLAASCGLLAANLYYAQPLI
                     GLIAPSVGLDAKAASLIVTFTQCGYCAGLALLVPLGDLIENRRLIVTTMCCAALAMGL
                     AAVAPSGSLFLFACLLIGLLSVAAQMLVPIAAHLAPDASRGRMVGNVMSGLLAGIMLA
                     RPVSSLIADVLGWRAVFVASAAAIAALAVVMRALLPARRPNADHHYFALIGSLWTLFR
                     ETPVLRRRAAYQAFLFAAFSLFWTAVPLELAGPTFGLSQRGIGFFALAGAAGAVSAPI
                     AGRLADRGWSRAVSGLSMAIIAVSFLLAQLGEAGSMAALIAAGLLIDGSVQANQVVGQ
                     RAIYALGAEARSRLNGLYVALFFIGGAAGSAIASLVYALGGWRWICWIGFAFPIAGLA
                     LFATEFTGKRGKNLSAAARSLSPDQ"
     gene            6239..6832
                     /locus_tag="VKU03_02320"
     CDS             6239..6832
                     /locus_tag="VKU03_02320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HLJ70123.1"
                     /translation="MAPTIDFWFDFASTYSYPAAMRAEALAEAAGVKLNWRPFLLGPI
                     FVAQGWRDSPFNLYPRKGRYMWRDLERICARENLPLKRPDVFPQNSLLAARCALALDA
                     AGRPAFARAVFAAEFAEGRRIDDPATLAALLAAPDLRESALAEASSERVKQALRAETE
                     EAQRRDLFGAPSFLTQDGELFWGNDRLEAAIAWAAAH"
     gene            6931..8208
                     /gene="tcuC"
                     /locus_tag="VKU03_02325"
     CDS             6931..8208
                     /gene="tcuC"
                     /locus_tag="VKU03_02325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tricarballylate/proton symporter TcuC"
                     /protein_id="HLJ70124.1"
                     /translation="MPPSKASSSKIGAVLRATAGNFLEMFDFFLFGIYAAPIGKAFFA
                     TGSEFADTSIAFVVFWIGALMRPLGAIYLGAYVDSVGRRKGLIATLSIMAVGTIIIAV
                     TPSYAAIGLAAPIVVVIGRLLQGFSAGVELGGVSVYLAEIAKPGTKGFVTSFQSASQQ
                     VAVFVAALIGYAVNHLLAPADILAWGWRIPFAIGCLIVPMIFALRRSLQETPEFLARK
                     RHPAPAEIYRAVAANWRVVALGMLMVMLTTVMFYFITVYTPDFGKALHMSASDALLAT
                     LLVAAANFFWLPVGGALSDRVGRAPVLLGVAALVLASAFPALRWLAVEPGFGKLLTVE
                     MLFSLCFGLYNGAMVAALTEVVPADVRASCFALAYSLATALFGTATPFVSKMLIRNFG
                     AAAPALWLMLAAACAFAAVAALYRRAIAAPVRA"
     gene            8273..9058
                     /locus_tag="VKU03_02330"
     CDS             8273..9058
                     /locus_tag="VKU03_02330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00266 family protein"
                     /protein_id="HLJ70125.1"
                     /translation="MRFEISGTIMQTVAIDLSPGETIYSQTNTMAWMNDAVQMDTHTG
                     GGFLAGLKRSFGGGSFFVTDFTATGAGHVAFAPRFPGTIFPAQLQAGQSLICRKETFL
                     CAQKSVSLELAWQKRIGAGFFGGDGFILQKVSGPGVVWLDLSGEVVERDLAPGERLLV
                     HAGHVGVFEPSVGFDIQMVRGFKNILFGGEGLFLATLTGPGHIWLQSMPIINLAEEIG
                     RYLPQNSGESAASSVGGMAATGAAIGVAGGLLGSLLGSGDNKT"
     gene            9144..9584
                     /locus_tag="VKU03_02335"
     CDS             9144..9584
                     /locus_tag="VKU03_02335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HLJ70126.1"
                     /translation="MIAKSSLYDRDFYAWSKKQAALLRAGKLQQADIEHIAEEIESMG
                     KTEKRELISRLTVLLLHLLKWRFQPAKRAASWEASVKVQRNRLIDHLDDNPSLRPLAP
                     AALATAYRDAVIEAVAETGLPGPMFPETCPWTLEQILSAEFWPE"
     gene            complement(9827..>12076)
                     /gene="mfd"
                     /locus_tag="VKU03_02340"
     CDS             complement(9827..>12076)
                     /gene="mfd"
                     /locus_tag="VKU03_02340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003690 - double-stranded DNA binding
                     [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription-repair coupling factor"
                     /protein_id="HLJ70127.1"
                     /translation="LSHVIAEHGLKSIELVSSLPQAKAARAGALPLAVIALEQGFETD
                     DLAVIGEQDILGDRLVRPRKAKRRAQDMLAEIASLAAGDLVVHIDHGIGRFVGLQTIE
                     AAGAPHDCLELHYAGGDRLYLPVENLELLSRYGSEDSNVALDKLGASGWQTRKARLKK
                     RVREMAAELIRIAAARQTREAPRLAPPDGLYDEFCARFPYDETQDQDNAIEATLDDLG
                     VGRPMDRLICGDVGFGKTEVALRAAFVAAMGGKQTAIVVPTTLLARQHTKNFQTRFAG
                     LPVKIGQLSRMVGAAEQREVKQGLAEGTIDIVIGTHAVLGKTVKFKDLGLVIIDEEQH
                     FGVGHKERLKELRAEVHVLTLSATPIPRTLQLAMTGVRELSIIATPPVDRLAVRTFVT
                     PFDALIVREALLRERYRGGQSFYVCPRIDDLEEAASFLRQNVPEAKFVVAHGQMASSE
                     LEDKIGAFYDGKYDILLSTSIVESGLDIPTANTLIVHRADMFGLAQLYQLRGRVGRSK
                     TRAYALFTVPANKPITPQAEKRLQVLQSLDTLGAGFQLASHDLDIRGAGNLLGEEQSG
                     HIKEVGYELYQQMVQEAVERLKAGVEEPAQDLWSPSISLGAPVTIPEEYIPDLATRLN
                     LYRRLSALESDEDIEAMGAEIVDRFGPTPPEVGLLLKLVTIKALCRRANVEKIDAGPK
                     GATLAFRDNAFANPGALVRYVETQAPLAKVRPDMRVVFIRDFDTLEARLAGVLEIMRD
                     LAKIAGSKK"
BASE COUNT         2061 a         3985 c         3893 g         2137 t
ORIGIN      
        1 aagatcggcg cgcctgccgt cgggcagcga cgcgtcggcc aggacgagaa ccgtcacgcg
       61 atcaacttca ttcgtcgtgc ggcgacgacc tggtacagac gatccccgta aagcctgccc
      121 tcctcgcgcg ccgaaaagac caggctcaga tcatgctcgt cgtccgactt gaccgcggcg
      181 gccgcgattt cctcccaatc gggacatggc gcgcgtcgcc aggcttcgag cgtctccgta
      241 tccggcaggc cgggaaagcc gatcttcgga tagagggcgg cgatcgattg ccagaaatag
      301 cgcaaggcga gatcgggatc gggcgtgatc ggcgcgatca gccgcagcca gtggcagccg
      361 gtcagcgcat gcagggcgca aaagtccatg gttccggcgt agagcgcgag cgaggctttc
      421 ctgacccgct ggaacgtccc aggcccgatc gccagccaat cgacgatcgg cgcgaattcc
      481 ggcttctgcg cgatcgcgcg catgaagtgc cagagcagat cgagctccgt ctcgacatgg
      541 cgaaacgtct cgatcggctg cattcgaacg agcacctcga ccggatcgtc ggtgatcggc
      601 ggcgcgccgc tggcgcggcc gagctcaagg tacgtcgccg cccaatagcc gagcgcggcg
      661 gcgatctcgg cctcgtcgtt gcgcatcgcg ccataggcga ggcgcatcag cccatgcgtg
      721 gcgcttgccg cgaggcccgg caggagtctc ggcaggtaga gcccggccgc ggcgcgcgcg
      781 ccgagccggg cgacttcctc ctggaagaaa gcgcgatagt ccgcctcccg ctcgcgcgcg
      841 ccgagcgcct cggtccaagc ttcgcgcgcg atcggcgcaa ccggaggcgg cggcggacac
      901 aggcgattga tctcgcgata ggtcgcgaaa tactccgcca ggcgccggtc cgaaccgccg
      961 agacggcgca tcgcgaccaa caccattggc aggtgattgg cgaggaagag cgggaattcc
     1021 gcgctgaact ttcgcccttc gtcgagcagg cgctcgagct cggtctcctc acgggtgaaa
     1081 tcgccgacag cggaacggag cgctgcggtc attgtgctcc tccatttcaa tcgggcggcg
     1141 aatggccccg ccggcgccgg ctcgccgtcc gcaacgcgtt ctgcgaagcg aacagggaga
     1201 gacgcggaag gcgaacattg ccgttcgata tcaagcatat agacatcgct ttggaaacgc
     1261 gccatcctcc ctgcgaaacg gggtgcatct tatgcatggc cctacgcttt gccaactctc
     1321 gcgaaagtag gagagagtcc atgaaagcgc gtcgaatact ggggatcgcc gcggcgattt
     1381 cgctcgcgtc tttggcgcaa gccaaagccg ccgataagcc ttatgacggc gtcacgctga
     1441 cgctcgcctc gcaaaacgat cagttcgcga cggtgatggc cgatctcgcg ccgaaattca
     1501 aagaagcgac cggcgtcact gtcaaggtcg acatcctgaa ctatcccgag ctcctgagta
     1561 aggtcaccgc ggattttatc ggccatacca agggctatga tctggcgacg atggacatcg
     1621 tgtgggccgg gcaattcgcc gagggcggct atacggtcga gctcagcgat tggatcaagc
     1681 gcgacgccgc cgaaatccat ccggacgaca tttatcccgt tctgatgtcg gcgctcggca
     1741 atttcaaagg caagcaagtc gcctttccgt tcgccggcta cgccaatgtg ctcgcctatc
     1801 gcaccgatct ctacgaagcg gcgggattga agccgccgca gaccatggag gacatggtcg
     1861 ccaacgccta caagctcacc gatccgccga aacattacgg cttcgtcgcc aatggccaga
     1921 aaggtccggc ggtggcgcag gattggatgc agtacaacaa tgaaatgggc ggctcgattc
     1981 tcgggccgga cggcaagccg gcgctgaatt cggcggccaa catcaagagc ctcaccgtct
     2041 acaaggagct cttcgacaaa tgcgcgccgc ccggcgccgc ggatttcgat tggggcggcc
     2101 gcgaggaagc gttccgccag ggccttgccg cgcagatgca gacctggtcg atcggcgccg
     2161 ccagctattc cgatccggaa aagtccaaaa tcgtcggcaa ggtcgcgatc accgtcgcgc
     2221 cgaccggcaa aggcgtgccg aagaaatacg gcgtcggcgg ctggggcatg tcgatcaacg
     2281 ccgacatcga ccagaagcat aaagaggcgg cgtggctgtg gatcaaatgg ctgaccagcc
     2341 cggccattca caagcagctc aatctcatgg gcgcgggcag ctatttgcgg attagcgaga
     2401 cccacgatcc cgaactcgtc gcgaagttcc ccttcctgcc ggtgatcgac gaagtgttca
     2461 agcatggcga cggcgactat cggccgcgca tcccgcaata tccgcagatc caggatctgc
     2521 tcggcacggc ggtcaacgcc gttctcgtcg gccacgagga cccgaaaaag gcgcttgacg
     2581 aggcgcaggc cgaagccgtg aaactgttct gagaagcaag gacccgtcgc gattggctat
     2641 tgccgaactg cgtcccgaaa agagcggcgg ccggacatcg agtccggccg tcgccgttcc
     2701 ggcgcggccc tggctgacct atccgcggcg gcgccaaatt tttctggtcc tgctcggcgc
     2761 gccggcgctg atctatgtgc tgctggtcgc gatctggccg ctggcgcgcg gcatcggcta
     2821 cagcctctat gattacaatc tgctgcggcc ggcgcgaacc gcctttgtcg gcttcggcaa
     2881 ttacagcgcg ctctgggcga acgacgccgc gcgcaacgcg atcgaaacga cggcgatgtt
     2941 caccatcgcc gccgtcgcgg tggagttcat cctcggcctc ggcctggcgc tgctgctttg
     3001 gcgcgacagc gcctttcagc gcgtctgcct cacccttctg ctcattccgg tgacggtgac
     3061 gccgatcgcg gtcgggttga tttttcgcgc gctgctcgcg cccgattatg gcctgatcgg
     3121 ctattatctc gccgcctggg gcctttccga tccgcatggg ctgctcggcg atccggccag
     3181 cgcgctttgg accttagtgg cggtcgacgc ctggcaatgg acgccgttca tggcgctgat
     3241 ccttctcgcc ggtctcaagg ccctgccgac cgagctgctg gaggcggccg aggtcgatgg
     3301 cgcgacggcg ctgcagcgct tgcgcctgat catcctgcca atgctgctgc cggcggcgtt
     3361 tcttgcgctg gtgctgcgga cgatggacgc gttccgcatt ttcgacatca tctactcgac
     3421 caccaatggc ggccccgccg acgcgaccaa cgtcttgatg gtctacgccg tgaaacaggg
     3481 gctcgaattt ttcaatatcg gcttcgcctc ggccatcgcc aatctgatga tcgtctgcat
     3541 cgcgatcttc tcggcggcgt tcatcgtcct ggtgcggcga gccgacaaga gggccaacca
     3601 atcatgagcc gctccccctc cgcctcggcc gtgatcgcgg agcgaagcgc gctggtcgtc
     3661 atcgtcgcga tcttcctcgc gcccgtcgcc tggatgattt cgaccgccta caagccgccg
     3721 cgcgatattt tcagcatccc gccgaccctc tccttcacgc cgacctgggc caatttcgcc
     3781 aatgtgtttc gctactttga tctgttccat ctcatcgaaa gcagcctcgc catcagcctc
     3841 ggcgcggcgg cgctttcgct tctgatcggc gcgccggccg gctacgcctt ggcgcgggcg
     3901 cagacgaaat gggcgatcgg cctcgcctat ttctttctcg ccgtgcgcac ggtgccgccg
     3961 gtggcgacgc tgctcccctt ctatctgatg atgcgcgaca tcggcctgct cggcacatgg
     4021 ggcgcggtga tcctgatcga cgcgacgctc aacaccgcct tcgtcgtctg gatgatgttc
     4081 tcgtatttcc gcgcgttgcc gaaagaaatg gaggaggcgg cgctgaccga cggctgcagc
     4141 ctgctcggca gctttcttcg catctcgctg ccgctggtca aaccgggcct cgtcgcgagc
     4201 gcgctgcttt gcgtcatctt cgcctggaac gatttcctct attcgtcctt cctgacgcgc
     4261 gccgaaagca agccgatctc ggtcgccctg ctcaccgcct acggcaccaa ggacatttcc
     4321 tggggcacgc tcggcgcgct ggcgcacttt tcgaccatcc cgatcgtcgc gatcgcgctt
     4381 gtgctcaacc gccatttcgt ccagggcctg accaggggga ttcactgatc gggcgacagg
     4441 cttcgagcgg ccgcgctcag gttcttgccg cgtttgccgg taaattccgt cgcgaaaagc
     4501 gccagcccgg cgatcggaaa ggcgaagccg atccagcata tccagcgcca gccgccaagc
     4561 gcgtagacca ggctggcgat cgccgagccg gccgcgccgc cgatgaagaa cagcgcgaca
     4621 tagagcccgt tgaggcggct gcgcgcctcg gcgccgagcg catagatggc gcgctggccg
     4681 acgacctggt tggcctggac cgagccgtcg atcagcaagc cggcggcgat cagggcggcc
     4741 atagagccgg cctcgcccag ttgagccaat aggaacgaaa ccgcgatgat cgccatcgac
     4801 aatccgctca ccgcccgcga ccagccgcga tcggcgagcc ggccggcgat cggcgcggag
     4861 acggcgccgg ccgcgccggc cagcgcgaaa aagccgatgc cgcgctgcga gagaccgaag
     4921 gtcgggccgg cgagctccag cggaaccgcc gtccagaaca ggctgaaggc tgcgaacaag
     4981 aacgcctggt acgccgcgcg gcgacgcagc acaggcgttt cccggaacag cgtccacagc
     5041 gagccgatca acgcgaaata gtggtggtcg gcgttcggcc gccgcgccgg cagcaaggcg
     5101 cgcatcacca cggccagggc cgcgatcgcg gcggcggagg cgacgaagac ggcgcgccag
     5161 ccgagcacgt cggcgatcag gctcgagacc ggccgcgcca gcatgatccc ggcgagcagc
     5221 ccgctcatca cattgccgac catgcggccg cggctcgcgt ccggcgcgag atgggccgcg
     5281 atcggcacca gcatttgcgc cgccaccgag aggaggccga tcaacaggca ggcgaaaagg
     5341 aaaagcgagc ccgaaggcgc gaccgccgcc aggcccatcg ccaaagccgc gcaacacatc
     5401 gtcgtcacga tcagccggcg attctcgatc agatcgccga gaggaaccag cagggccagt
     5461 ccggcgcagt agccgcattg ggtgaaggtc acgatcaggc tcgcagcctt tgcgtcgagc
     5521 ccgaccgacg gcgcgatgag cccgatcagc ggctgggcgt aatagagatt ggcggcgagc
     5581 aagccgcagg acgccgccaa aagaaaggtc atccagccgg gcgcgccctg ctcctgatcg
     5641 accgcgtcgg ttttttcgag aatcaaatcg gtcatttgct cttaaggtct tctcatcgta
     5701 ccgcgctcgg ccgctccaac gcgtcaggcc gacgcgggat cgctgcggcg tctgtctcga
     5761 tgaaggcgtg tggagttcgc ccggaacgag cgaaacgatc gaccgcggac gctcgcgccg
     5821 aaagcggcgg atcgtccgcg cggctcagga ccggcttcta taaaccggaa aagcggaccg
     5881 cccacttcac aatcgcgtca ggcttgacgc cggagccgca gcgctgaaat cagccggccg
     5941 cgcggcgccg gccggcgctg tcgtcgctaa agctacgcgc cgctttttcg aggccggctt
     6001 cgatcagaag gttgcggaaa gccgtccatt gctcgagcga agcgccgatg ctcggcgaga
     6061 aagttgcgga ttcgccccga ccgcagggag aaagatcgac gcgcggaacg gcgccgacat
     6121 atttaaggct tgtaaaaaag gtcttatcaa aaaccgttcg catgattttc cgccggcgga
     6181 tttgaaggct gaacgccggc attgccggtc cgccgcacgg cgacgggagg attgcccgat
     6241 ggctccgacc atcgacttct ggttcgattt cgccagcacc tattcctatc cggccgcgat
     6301 gcgggcggag gcgctggctg aagcggccgg cgtcaagctc aattggcggc ctttccttct
     6361 cgggccgatc ttcgtcgcgc aaggttggcg cgattcgccg ttcaacctct atccgcgcaa
     6421 gggccgctat atgtggcggg atctggagcg gatttgcgcg cgcgagaacc tgccgctgaa
     6481 gcggcccgac gtctttccgc agaactcgct gctcgccgcg cgctgcgcgc tggcgctcga
     6541 cgccgccggg cggccggctt tcgcgcgggc ggtcttcgcc gccgaattcg ccgaaggccg
     6601 gcggatcgac gatccggcga cgctcgccgc ccttctcgcc gcgccggatc tgcgggaaag
     6661 cgcgctcgcg gaagcatcga gcgagcgcgt caagcaggcg ctgcgcgccg agacggagga
     6721 agcgcagcgc cgcgaccttt tcggcgcgcc gagctttttg acgcaggacg gcgaactctt
     6781 ctggggcaat gaccgcctgg aggcggcgat cgcctgggcc gccgcgcatt gagcccgcca
     6841 atcgctcggc ttgatccgcc ttcgcttcga agacaaagat aggccattcg ctcggcaggc
     6901 tccgcccgcc gcgaggggga gcgcaaggcc atgccgccat ccaaggcttc atcgtcgaaa
     6961 atcggcgccg tcctgcgcgc gaccgccggc aatttcctcg aaatgttcga cttcttcctg
     7021 ttcggcatct atgcggcgcc gatcggcaag gcgtttttcg cgacaggcag cgaattcgcc
     7081 gacacgtcga tcgccttcgt cgtcttctgg atcggcgcgt tgatgcgccc gctgggcgcg
     7141 atctatctcg gcgcctatgt cgacagcgtc ggccgccgca agggactgat cgcgaccctg
     7201 tcgattatgg cggtcggcac gatcatcatc gccgtcacgc cgagctatgc ggcgatcggc
     7261 ctcgccgccc cgatcgtcgt ggtgatcggc cgcttgctgc agggcttctc cgccggcgtc
     7321 gagctcggcg gcgtttccgt ctatctcgcg gaaatcgcca agcccggcac aaaagggttc
     7381 gtcacctcgt tccaatccgc gagccagcaa gtggcggtct tcgtcgccgc gctgatcggc
     7441 tatgcggtca atcatctcct cgcgccggcc gacattctcg cctggggctg gcgcattccc
     7501 ttcgcgatcg gctgcttgat cgtgccgatg attttcgcgc tgcggcgctc gctgcaggag
     7561 acgccggaat ttcttgcccg aaagcgccat ccggcgccgg cggaaatcta tcgcgccgtc
     7621 gccgccaatt ggcgcgtcgt cgcgctcggc atgctgatgg tcatgctgac gacggtgatg
     7681 ttctatttca tcaccgtcta cacgccagac ttcggcaagg ccctgcatat gtcggcgagc
     7741 gacgcgttgc tggcgacgct cctcgtcgcc gccgcaaatt tcttttggct gccggtcggc
     7801 ggcgctcttt ccgatcgggt tgggcgcgcg ccggttctgc ttggcgtcgc cgctctggtt
     7861 cttgcatcgg cctttccagc attgcgctgg ctcgccgtcg agccgggttt cggcaagctg
     7921 ctgacggtcg aaatgctgtt ctccctatgc ttcggcctct acaatggcgc gatggtggcg
     7981 gcgctgacgg aggtcgtgcc ggccgacgtg cgcgcctcct gctttgcgct ggcttattcg
     8041 ctcgcgacgg cgcttttcgg cacggcgacg ccgttcgtct cgaaaatgct gatccgcaat
     8101 ttcggcgccg ccgcgccggc gctttggctg atgctggcgg cggcctgcgc tttcgcggcg
     8161 gtcgcggccc tttaccggcg cgccatcgcc gcgcccgtcc gcgcttgatc cctgcgatgg
     8221 actcgccgcc cgaaggcggc tagcgatagg acgctcgacc tgggggacgc gcatgcgctt
     8281 cgagatatcc ggcacgatca tgcaaaccgt ggcgatcgac ctttcgcccg gcgaaacgat
     8341 ttacagccag accaacacga tggcctggat gaacgacgcc gtccaaatgg atacgcatac
     8401 cggcggcggc tttctcgccg gcttgaagcg ctcgttcggc ggcggctcgt tcttcgtcac
     8461 cgacttcacc gcgaccggcg ccggccatgt cgcgttcgcg ccgcgctttc ccggcacgat
     8521 ttttcccgcc caattgcagg ccggccaatc gctgatctgc cgcaaggaga cgttcctctg
     8581 cgcgcagaaa tcggtgtcgc tggagctcgc ctggcagaag cgcatcggcg ccggcttttt
     8641 cggcggtgac ggcttcatcc tccagaaggt cagcgggcca ggcgtcgtct ggctcgacct
     8701 ttccggcgag gtggtcgagc gcgatctcgc cccgggggag cggcttttgg tccatgccgg
     8761 ccatgtcggc gtgttcgagc cgagcgtcgg cttcgacatc cagatggtgc gcggcttcaa
     8821 gaacatcctg ttcggcggcg aaggcctgtt cctcgcgacg ctcaccggcc ccggccatat
     8881 ctggctgcaa agcatgccga tcatcaatct tgccgaggag atcggccgct atctgccgca
     8941 aaattccggc gagagcgcgg cttcgagcgt cggcggaatg gccgcgaccg gcgcggcgat
     9001 cggcgtcgcc ggcgggctgc tcggcagcct gctcggcagc ggggacaata aaacctgacg
     9061 cgcgccaagc gtcgagccgg gatgggcttt cactccatac ggcgcgcatg gacgccagcg
     9121 tcatcaagcg gtaaaattac cgaatgatcg caaaaagctc tctctacgat cgagatttct
     9181 acgcttggag caagaaacaa gccgcgctct tgcgggccgg caagctccaa caggccgaca
     9241 ttgaacatat cgccgaggaa atcgagagca tgggcaagac cgaaaagcgc gagctgatca
     9301 gccgcctcac ggtcttgttg cttcatctac tcaaatggcg cttccagccc gccaagcgcg
     9361 ccgcaagctg ggaggcgagc gtcaaagtcc agcggaaccg cctgatcgat catctcgacg
     9421 acaaccccag cctaaggccg ttggcgccgg ccgcgctcgc aaccgcctat cgtgacgcgg
     9481 tcatcgaggc ggttgccgag accgggttgc cggggccgat gtttccggaa acctgtccct
     9541 ggacgcttga gcaaatcttg agcgcagagt tttggccgga gtgagattgg cgctgcgcgt
     9601 gggggagtaa tgctggctgc cggctcgagg agacaatccg cctgatatag acattctgat
     9661 cgaaattgcc tcggaggcat cctgttcaca tacgttgtgt cgaggcagcg aatcggagca
     9721 gatcgtactc gttcgcgtca gcatcgtttc gagcgcagag tcatccataa atctcttcac
     9781 ttgaatggat tgcttcgttg cttcgctcct cgcaatcacg gcggcgctac ttcttgctcc
     9841 ccgcgatctt cgccaaatcc cgcatgatct ccagcacgcc ggcgagccgc gcctccagcg
     9901 tgtcgaaatc gcggatgaag acgacgcgca tgtcgggtcg caccttcgcg agcggggctt
     9961 gcgtctcgac gtagcgcacc aaagcccccg gattggcgaa ggcattgtcg cggaaggcca
    10021 gcgtcgcgcc tttcgggccg gcgtcgatct tctcgacatt ggcgcgccgg cacaaagcct
    10081 tgatcgtcac cagcttcagc agcagaccga cttccggcgg cgtcgggccg aaccggtcga
    10141 cgatctcggc gcccatggcc tcgatgtctt cgtcgctctc gagcgccgag aggcggcggt
    10201 agagattgag cctggtggcg aggtctggga tatactcttc cgggatcgtc accggcgcgc
    10261 cgagcgagat gctcggcgac cagaggtcct gcgccggctc ctcgaccccg gctttgaggc
    10321 gctcgaccgc ctcctgcacc atctgctgat agagctcgta gccgacctcc ttgatatggc
    10381 cggactgctc ctcgccgagc aggttgccgg cgccgcgaat gtcgaggtcg tggctcgcca
    10441 attggaagcc ggcgccgagc gtgtcgagcg attgcagcac ctgcaggcgc ttttcggcct
    10501 gcggcgtgat cggcttgttg gcgggcacgg tgaacagcgc ataggcgcgg gtcttcgagc
    10561 gcccgaccct gccccgcaac tggtaaagct gggcgaggcc gaacatgtcg gcgcgatgga
    10621 cgatcagcgt gttggcggtc ggaatgtcga ggccggattc gacgatcgag gtggaaagga
    10681 gaatgtcgta cttgccgtca tagaaggcgc cgatcttgtc ctcgagctcg ctcgacgcca
    10741 tttggccatg cgcgacgacg aatttcgcct ccggcacgtt ctgacgcagg aagctcgccg
    10801 cctcctccag gtcgtcgatg cgcgggcaga cgtagaagct ctggccgccg cgatagcgct
    10861 cgcgcaacag cgcctcgcgc acgatcagcg catcgaacgg cgtgacgaac gtgcgcacgg
    10921 cgagccggtc gaccggcggc gtcgcgatga tcgagagctc gcgcacgccg gtcatggcaa
    10981 gctgcaacgt gcgcgggatc ggggtcgccg acagggtcag gacatggact tcggcgcgca
    11041 gctccttgag ccgctccttg tgtccgacgc cgaaatgctg ctcctcgtcg atgatgacca
    11101 ggccgagatc cttgaacttg accgtcttgc cgaggacggc atgggtgccg atgacgatgt
    11161 cgatcgtccc ttcagcgagg ccttgcttga cctcgcgctg ctcggcggcg ccgaccatgc
    11221 gcgagagctg gccgatcttg accggcaggc cggcgaagcg cgtctggaag ttcttcgtat
    11281 gctggcgcgc cagcaaggtg gtcggcacga cgatcgccgt ctgcttgccg cccatcgcgg
    11341 cgacgaaggc ggcgcgcagc gccacttcgg tcttgccgaa gccgacatcg ccgcaaatga
    11401 ggcgatccat cggccggccg acgccgagat cgtccaacgt cgcctcgatc gcgttgtcct
    11461 gatcctgcgt ctcgtcatag ggaaagcgcg cgcagaattc gtcgtagagg ccatcgggcg
    11521 gagccaagcg cggcgcttcc cgcgtctggc gggcggcggc gatgcggatg agctcggccg
    11581 ccatttcgcg gacgcgcttt ttcagcctgg ccttgcgcgt ctgccagccg gaagcgccga
    11641 gcttgtcgag cgcgacattc gaatcctccg agccgtagcg cgacagaagc tcgagattct
    11701 cgaccggcag gtacagccgg tcgccgccgg catagtgcaa ttccaggcag tcatgcggcg
    11761 cgccggccgc ctcgatcgtc tgcaggccga cgaaacggcc gatgccgtga tcgatatgga
    11821 cgacgagatc gccggcggcg agcgacgcga tttccgccag catgtcctgg gcgcggcgct
    11881 tggccttgcg cggcctgacc aggcggtcgc cgagaatgtc ctgctcgccg atcacggcga
    11941 gatcgtccgt ctcgaagccc tgctccaggg cgatgacggc gagcggcaag gcgcccgccc
    12001 gcgccgcctt ggcctggggc agagacgaga cgagttcgat cgatttcagc ccatgctcgg
    12061 cgatgacatg cgacag
//
LOCUS       DATGJW010000078         8110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1051070, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000078 DATGJW010000000
VERSION     DATGJW010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8110
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1051070"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(463..744)
                     /locus_tag="VKU03_02345"
     CDS             complement(463..744)
                     /locus_tag="VKU03_02345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HLJ70128.1"
                     /translation="MAARVASRDFVRQLEGFSLTTAEILYRLPDHPALLQSYIWQDYD
                     VAPQFPVLMKFLTFWREKLEGPLHSVRVAHCRLIGPAELGAVGQEFVLH"
     gene            complement(840..1508)
                     /locus_tag="VKU03_02350"
     CDS             complement(840..1508)
                     /locus_tag="VKU03_02350"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLJ70129.1"
                     /translation="MSDERPKTQAIVMVKIAAILLAAGRSARFAAAGGREATKLAASV
                     AGKPLARYAAEAALASAARPVVVVTGHARDAVEAALHGLRLRFVHNPAFASGLASSLK
                     AGLAALPGDIEGAVVLLADMPAVAPALIDRLAAAFAAAPDALAAIPVAEGRRGNPVLL
                     GRALFPAVAALDGDEGARRLLAGLPPGRVVEIAAGDDGATLDVDTPEALAAARLKFGR
                     GGDH"
     gene            1648..4905
                     /locus_tag="VKU03_02355"
     CDS             1648..4905
                     /locus_tag="VKU03_02355"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLJ70130.1"
                     /translation="MDLSGVALYWLGRPNGAPPAALRRFGLKVELPTRAPDGAAIVFL
                     DAAADEAGSAFADIRAKLGGEALIVLIADEEKPALIGGEVDDLCRRDALAKPDLVLAR
                     LAARARERVRAARSRAALERRCAQIQAGLDALPTPIFIKDADIRYTVCNKAFQRFVGL
                     SAEQIIGQRVYGVSRPERAKYYEATDRALLAAGGEQIYETSVRYADGSRHEVMFHKAA
                     FRDEAGAVVGMAGVMLDINQRKRAEAALHESELRYRDVFEHASDCIALIDVTADGRYV
                     LSAANPAARRACGLAEDEGLEQPIELSMATAAAAPFMERLAECVEARRTTRYETLFKG
                     PAGLRTFIVNLVPVAQDGEPARRIIAIARDMTDEREVERFRSERERDFRTLVDNSPDA
                     IVRYDRACRRVFSNRAIERNRNIDPEILLGKTPVESQILGDREQERLYQDWLFAVMAA
                     GKPDERQFFYVRPSGERRIATIRAIPERGANGEVVSILSIGTDADQRIRAERQLRQRE
                     QEFRSLVENSPDMIVRYDTGLRRVYVNPAAQKRLGAGKVALGGTPLDGPNLQVDLYMG
                     KLREAIVFGRECELESSFQASDGAVRWSHSRIVPEFDQNGKVVSVLGIGRDITEIVES
                     RRKIQRLAFYDSLTDLPNRALLSERIAEIASRPDGNGAFALMLLDLDRFKEINDTFGH
                     ALGDELLCKVAARLRRCIRKNDTISRLGGDEFAILLPDPAKDRDFAAIAAKILRAFAR
                     PFQLPGREIGIFASIGVAECAGEAIDVDTLFKQADSAMYHAKRLGRNNFQFFTPEMTA
                     RNVWRMAVETHLRKAIARGELDLYFQPRVALKTQRILGAEALLRWRHSELGFLTPDKF
                     IAIAEESDQIVEIGRWVLAEACRRAAEWNKAAPAPIQVAVNLSSRQFVMSDLVASLRE
                     ILDESDCRPEWLELEITESLLLEDNKSVRETLEAFRDIGASIAIDDFGTGFSALSYLT
                     RFPISTLKIDRSFISDVDRDPKRAELVKAIIGISTALELKLIAEGVENQAQVDFLLRA
                     GCEEAQGFLFGRPMPEPEFRSRLRGALPAGGDSRPRLRRAAGAPLLRAVRAPQRA"
     gene            complement(4893..5924)
                     /locus_tag="VKU03_02360"
     CDS             complement(4893..5924)
                     /locus_tag="VKU03_02360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLJ70131.1"
                     /translation="MKFGPVPVAEAEGAIVAHTIRQDSLILKKGQTVTREHMEKLAAA
                     GIAEIIAARLDAEDVGEDEAARLLAEKLAGPHIRLERAFTGRVNLFAEAAGLLVVDAG
                     AIDRLNGVDEAITVATLPRYHVVAPGDMAATVKIIPFAARRAALDAGLAGAERGAVAV
                     APFRPLRVGVVSTLLPGLKPSVVAKTLRILEERLAPAAARIEREERTPHDAAALAQAL
                     DRVAPDSDLVIVFGASAITDRRDVIPAAIENRGGRIDHFGMPVDPGNLLLLGELAGRP
                     VIGAPGCARSPKENGFDWVLQRLLAGVPVGSKDIRAMGVGGLLMEIPQRPQPRLGERG
                     AANPPDQAR"
     gene            complement(5921..6625)
                     /locus_tag="VKU03_02365"
     CDS             complement(5921..6625)
                     /locus_tag="VKU03_02365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HLJ70132.1"
                     /translation="MRLELLAALNAERAARRACVLITELAGGAQRLVKADDIAGDAME
                     AALEPALRSGKSAAVEFDGKSYFLTVQVPAVRLVLIGAVHISQALAPVAKLCGLDPVI
                     IDPRTAFASPERFPDVELVAEWPDEALDRAPLDRYTAIALLTHDPKIDDPALQRALKA
                     ECFYIGALGSKKTHARRLERMRAAGFSEAALARIHAPIGLDIGAVSPAEIAVAIAGEV
                     IGALRKKPLRAEEKAA"
     gene            complement(6625..6948)
                     /locus_tag="VKU03_02370"
     CDS             complement(6625..6948)
                     /locus_tag="VKU03_02370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452917.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="XdhC family protein"
                     /protein_id="HLJ70133.1"
                     /translation="MLSTEEDILAAAENWRSKGRDVAIATVIETWGSAPRPTGSHLVI
                     DGEGNFLGSVSGGCVEGAVVTEALDVIAAGEPKVLEFGVADETAWKVGLSCGGRIKVY
                     VERLD"
     gene            complement(7060..7674)
                     /locus_tag="VKU03_02375"
     CDS             complement(7060..7674)
                     /locus_tag="VKU03_02375"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ70134.1"
                     /translation="MGVVLNLSDTCLRSKIAVDLALSGLSLPALAGDRADDRIEAGPG
                     ARVSATIMLTDIVDSTRRAAAMGDESWSRLLDRHDDLAREIVRRYSGVLAKTTGDGVL
                     ATFDGPGCAVRCALTLQSAARLIGLALRVGVHAGEVELRRGDVRGVAVHAAARVMRKC
                     APGEVLVSRAVADLAAGTGLAFSARGAHELRGLPGQWDLYAARV"
     gene            complement(7803..>8110)
                     /locus_tag="VKU03_02380"
     CDS             complement(7803..>8110)
                     /locus_tag="VKU03_02380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836574.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLJ70135.1"
                     /translation="RVLGQGAIVLLFTDGLERDEPQELGREMERLRKSCRRLIWANPL
                     LRYGAFEAKAQGIRAMLPHVDEFRPIHNLANMADLCRALVEDRSHFADPKGWLRLSA"
BASE COUNT         1358 a         2775 c         2736 g         1241 t
ORIGIN      
        1 gcggcgccac gccggccgat cgccgtcaac ccgccatagc gccggcaagc tacgtgcaag
       61 atcatatccg cttgtgcggc gctatggcgg gtcctccgct tcgctccggc cctccgggtg
      121 actgcgatcg cccggcgtgg cagcgcggtg taggggattt gcgtcatcgt tgcatacgta
      181 ctgctcgcaa gagtctgtga gcagccccga ggtccgcatg ttttctgagt ctcggcgccg
      241 gaaactcaac gaggaaatct cgaaatcgcg ttatttcacg agccttcgtc gacgacgatg
      301 cgactcgcct ctcaactttc cccggatagc cctgcgtttc acggggagaa ggtggcgcga
      361 agcgccggat gagggggggc gcgggacgca gaaggtacgc acgcccgtcc ggagcggctc
      421 atcggcgggt tcggcgctcc ccctcatccg cgcgccgcgc ctctaatgca gcacgaattc
      481 ctgtccgacg gcgccgagct cggcggggcc gatgaggcgg caatgggcga cgcgcacgga
      541 atggagcggc ccttcgagct tttcgcgcca gaatgtcagg aacttcatca ggaccgggaa
      601 ttgcggcgcg acgtcgtagt cttgccaaat gtagctttgc agcaacgcgg gatgatccgg
      661 caggcggtaa agaatttccg cggtcgtcag gctgaagcct tcgagctgcc gcacgaaatc
      721 acgagatgca acgcgagccg ccattcgccg atctccttcg taataccctc cgaaaactta
      781 cggtccgcca atgcgttgac gagagaacga cattgcgcgc tcaaatgttt aatcaaatcc
      841 taatgatcgc cgccgcgccc aaattttaga cgcgccgcgg ccagcgcctc gggcgtgtcg
      901 acatcgagcg tcgcgccgtc gtcgccggcg gcgatttcga cgacgcggcc cggcggcagc
      961 ccggcgagca ggcggcgcgc gccttcgtcg ccatcgagcg ccgcgacggc gggaaagagc
     1021 gcccggccga gcaggacggg attgccgcgc cggccctcgg cgaccgggat cgccgccagg
     1081 gcgtccggcg ccgccgcaaa ggccgcagcg agacggtcga ttaaagccgg cgcgaccgcc
     1141 ggcatgtcgg cgagcaggac gaccgcgccc tcaatatcgc cgggcagagc cgcgagcccg
     1201 gccttcagcg acgaagcgag gccggaggcg aaagcggggt tatggacaaa gcgcaaccgc
     1261 aagccgtgca gcgccgcctc gaccgcgtcc cgcgcatggc cggtgacgac gacgaccgga
     1321 cgcgctgcgg aggcgagcgc cgcttcggcg gcgtagcgcg ccaagggctt gccggcgacc
     1381 gaagccgcca gcttggtcgc ctcccgcccg cccgcagcgg cgaaacgcgc cgagcggccg
     1441 gcggcgagga ggattgcggc tattttaacc atgacgatcg cttgcgtctt cggtctttca
     1501 tcagacaagg accggcggcc aatcctttaa ggctcccttc acggttggcg aaggaggatg
     1561 ccggcgattg gggggtcagc gcaacatcaa tcaagcaaga gacggccgcg ccgcgcgcgg
     1621 ccttgcgctg tctttgaggc gaagtcgatg gatttgagcg gcgtcgcatt gtactggctg
     1681 ggccgcccaa atggcgcgcc gccggcggcc ttgcggcgct tcggtctcaa ggtcgaattg
     1741 ccgacgcgcg cgccggacgg ggcggcgatc gtcttcctcg acgccgcggc cgacgaggcc
     1801 ggatcggcct tcgccgacat ccgcgccaag cttggcggcg aggcgttgat cgtcctcatc
     1861 gccgacgagg aaaaacccgc tttgatcggc ggcgaagtcg acgacctttg ccggcgcgac
     1921 gcgctggcca agccggacct cgttctcgcc cgcctcgcgg cccgcgcccg cgaacgcgtt
     1981 cgcgccgccc ggtcgcgcgc cgcgctggag cggcgctgcg cgcaaatcca ggccggcctc
     2041 gacgcgctgc cgacgccgat tttcatcaag gacgcggaca tccgctatac ggtctgcaac
     2101 aaggcgttcc agcgctttgt cggcttgtcg gccgagcaga tcatcggcca gcgcgtctac
     2161 ggcgtctcgc ggcccgaacg cgccaaatat tacgaggcga ccgaccgcgc tctgctcgcc
     2221 gccggcggcg agcagatcta cgaaacgagc gttcgctacg ccgacggctc ccgtcacgag
     2281 gtgatgttcc acaaggcggc gttccgcgac gaggccggcg ccgtcgtcgg catggccggc
     2341 gtgatgctcg acatcaacca gcgcaagcgc gccgaggcgg cgctgcatga aagcgagctg
     2401 cgctatcgcg acgtcttcga gcacgcctcc gattgcatcg cgctgatcga tgtgacggcg
     2461 gacggtcgct atgtcctttc cgcagccaat cccgccgcgc ggcgggcctg cggcctcgcc
     2521 gaagacgagg gcctcgaaca gccgatcgaa ttgagcatgg ccaccgccgc cgcggcgccg
     2581 ttcatggagc gcctcgccga atgcgtcgag gcgcggcgga ccacgcgcta cgagacgctt
     2641 ttcaaagggc cggccggcct gcgcaccttc atcgttaatc tcgtgccggt cgctcaggac
     2701 ggcgaacccg cgcggcggat catcgccatc gcccgcgaca tgaccgacga gcgcgaagtc
     2761 gagcgcttcc gcagcgagcg ggagcgcgat ttccgcacgc tcgtcgacaa ttcgcccgat
     2821 gcgatcgtcc gctacgatcg cgcctgccgc cgcgtcttct ccaaccgggc gatcgagcgc
     2881 aaccgcaaca tcgatccgga gatcctcctc ggcaagacgc cggtcgaatc gcaaatcctc
     2941 ggcgaccgcg agcaggagcg gctctaccag gattggctct tcgccgtgat ggcggcgggc
     3001 aagcccgacg aaagacaatt cttctatgtc aggccgtccg gagagcgccg catcgcgacg
     3061 atccgcgcca ttcccgagcg cggcgccaat ggcgaggttg tcagcatcct gtcgatcggc
     3121 accgacgccg accagcgcat cagggccgag cgccagctcc gtcagcgcga gcaggagttc
     3181 cgcagcctgg tcgagaactc tccggacatg atcgtcaggt acgatacagg gctcaggcgc
     3241 gtctatgtca atccggcggc gcaaaagcgc ctcggcgccg gcaaggtcgc gcttggcggc
     3301 acgccgctcg acggccccaa tctccaggtc gatctctata tgggcaagct gcgcgaggcg
     3361 atcgtcttcg gccgggaatg cgagctcgaa tcctcgttcc aggcgagcga cggcgcggtg
     3421 cgctggagcc attcgcggat cgtgccggaa ttcgaccaga acggcaaagt ggtcagcgtc
     3481 ctcggcatcg gccgcgacat caccgagatc gtcgagagcc gccgcaagat ccagcgcctc
     3541 gccttctatg attcgctgac cgatcttccc aaccgggcgc tgctcagcga gcggatcgcc
     3601 gagatcgcgt cgcggccgga cggcaacggc gccttcgcgc tgatgctgct cgatctcgac
     3661 cggttcaagg agatcaacga cacgttcgga cacgcgctgg gcgacgagct cttgtgcaaa
     3721 gtcgcggcgc gcctgcgccg ctgcatccgc aagaacgata cgatttcgcg gctcggcggc
     3781 gacgaattcg cgattctcct gcccgacccg gcgaaggacc gcgacttcgc cgcgatcgcg
     3841 gcgaagattt tgcgcgcctt cgcccggccc ttccaattgc cgggacggga aatcggcatc
     3901 ttcgccagca tcggcgtcgc cgaatgcgcc ggcgaggcga tcgacgtcga caccttgttc
     3961 aagcaggccg actcggcgat gtaccacgcc aagcggctgg gccggaacaa tttccagttc
     4021 ttcacgccgg aaatgacggc gcgcaatgtc tggcgcatgg cggtggagac ccatctgcgc
     4081 aaggcgatcg cgcgcggcga gctcgacctc tatttccagc cgcgggtggc gctgaagacg
     4141 caaaggattc ttggcgcgga ggcgctgctg cgctggcgcc atagcgagct cggcttcctg
     4201 acgcccgaca agttcatcgc catcgccgag gaatccgacc agattgtcga gatcgggcgc
     4261 tgggtgcttg ccgaggcctg ccggcgcgcc gccgaatgga acaaggcggc gccggcgccg
     4321 atccaggtcg cggtcaacct gtcgagccgg caattcgtga tgagcgacct cgtcgcctcc
     4381 ttgcgcgaga ttctggacga gagcgactgt cggccggaat ggctcgagct cgaaatcacc
     4441 gaaagcctcc tgctcgagga caacaagagc gtgcgcgaga cgctcgaagc tttccgcgac
     4501 attggcgcct cgatcgccat cgacgatttc ggcaccggct tttccgcgct gagctacctg
     4561 acgcgcttcc cgatcagcac gctgaaaatc gaccgcagct tcatcagcga cgtcgatcgc
     4621 gatccgaagc gcgccgagct cgtcaaggcg atcatcggca tatcgaccgc gctggagctg
     4681 aagctgatcg ccgagggcgt cgagaaccag gcgcaggtcg atttcctgct ccgcgccggc
     4741 tgcgaggagg cgcagggctt ccttttcggc cggccgatgc cggaacccga attcagaagc
     4801 cgcctgcgcg gcgcgctgcc ggcgggcggc gattcgcggc cccggctcag gcgcgcggct
     4861 ggcgcgccgt tgctgcgggc cgtgcgcgcg cctcagcggg cctgatcggg cggattggcc
     4921 gcgccgcgct cgccgagccg cggctgcgga cgttgcggga tttccatcag caggccgccg
     4981 acgcccatgg cgcggatgtc cttggagccg accggcacgc cggcgaggag gcgctgcagc
     5041 acccagtcga aaccgttttc tttcggcgag cgggcgcagc ccggcgcgcc gatcaccggc
     5101 cggccggcca attcgccgag cagaagcaga ttgccgggat cgactggcat gccgaaatga
     5161 tcgatgcgcc cgccgcgatt ctcgatcgcg gcgggaatga cgtcgcggcg atcggtgatc
     5221 gcggaggcgc cgaagacgat gacgagatcg ctgtccggcg cgaccctgtc caaggcttgc
     5281 gccaaggccg ccgcgtcatg cggcgtccgc tcctcgcgct cgatgcgcgc ggcggcgggc
     5341 gcgagccgct cctccagaat gcgcagcgtc ttggcgacga cgctcggctt gaggccgggc
     5401 agaagcgtcg agacgacccc gacccgcagc ggccggaacg gcgcgaccgc gaccgcgccg
     5461 cgctccgcgc cggcaaggcc cgcatcgagc gccgcgcgcc gcgcggcgaa tgggatgatc
     5521 ttgacggtgg ccgccatatc gccgggcgcg acgacgtgat agcggggcag cgtcgcgacc
     5581 gtgatcgcct cgtcgacgcc gttcagccgg tcgatggcgc ccgcatcgac gacgagaagg
     5641 ccggcggcct cggcgaagag attgacgcgg ccggtaaagg cccgttcgag ccggatatgc
     5701 ggcccggcga gcttttcagc gagcagccgc gccgcttcgt cctcgccgac atcctcggcg
     5761 tcgagccgcg cggcgatgat ttccgcaatc ccggccgcgg ccagcttttc catatgctcg
     5821 cgcgtgaccg tctgaccctt tttcaggatc agcgaatcct ggcggatcgt atgggcgacg
     5881 atcgcccctt ccgcctcggc gacgggaacc gggccgaatt tcacgccgcc ttttcctccg
     5941 cgcgcagcgg cttcttgcgc agggcgccga tcacctcgcc ggctatggcg acggcgatct
     6001 cggccgggct gaccgcgccg atatcgagcc cgatcggcgc atggatgcgg gcgagggccg
     6061 cttcggaaaa gcccgccgcg cgcatccgct cgagccgccg cgcatgcgtc ttcttggagc
     6121 cgagcgcgcc gatatagaaa cattccgcct tcagcgcccg ctgcagcgcc ggatcgtcga
     6181 tcttgggatc atgggtcaga agcgcgatcg cggtgtagcg gtcgagcggg gcgcgatcca
     6241 acgcctcgtc cggccattcg gcgacgagct cgacatccgg gaagcgctcc ggactggcga
     6301 aggcggtgcg cggatcgatg atcacggggt cgaggccgca aagcttggcg accggggcca
     6361 aggcctggct gatatgcacc gcgccgatca ggacgagccg caccgccggg acctggacgg
     6421 tgaggaaata gctcttgccg tcaaactcga cggcggcgct tttcccggac cgcagagccg
     6481 gctccagcgc cgcctccatc gcatcgccgg caatgtcgtc ggccttgacg aggcgctgcg
     6541 cgccgcccgc caattcggtg atcagcacgc aggcccgccg cgcggcgcgc tcggcgttca
     6601 gggcggcgag caattcgagc cgcatcagtc gagcctctcg acatagacct tgatgcggcc
     6661 gccgcaggac aggccgacct tccacgcggt ctcgtcggcg acgccgaact ccagcacttt
     6721 cggttcgccg gccgcgatca cgtcgagcgc ctcggtcacc accgcgcctt cgacgcagcc
     6781 gcccgagacc gagccgagga aattgccctc gccgtcgatc accagatggc tgccggtcgg
     6841 acgcggcgcc gagccccagg tttcgatgac ggtggcgatg gcgacgtcgc ggcccttgct
     6901 gcgccagttt tcagccgcgg cgaggatgtc ttcttcggtt gagagcatat ggtttcgctt
     6961 ccttcgagcg gccgcatgat agcgcaaagc gcggcgtgtt cagaagcgaa atggtcacgc
     7021 gggaataacg gcgcaagagg cggacccgcg ggcggcgcgc tagacgcgcg ccgcgtagag
     7081 gtcccactgt ccgggcagac ccctgagctc gtgcgcgccg cgcgccgaga aggccagtcc
     7141 ggtcccggcg gcgagatcgg cgaccgctcg cgacaccagg acttcgccag gcgcgcattt
     7201 gcgcatcacg cgggccgcgg catgaacggc gacgccgcgc acgtcgccgc gccgcaattc
     7261 gacctcgccg gcatggacgc cgacgcgcaa ggcgaggccg atcagcctcg ccgcgctttg
     7321 cagcgtcaaa gcgcagcgga cggcgcagcc tggcccgtcg aacgtcgcca ggacgccgtc
     7381 gccggtggtc ttggcgagaa cgccggaata gcggcggacg atttcccgag cgagatcgtc
     7441 atgccgatcg agcaggcggc tccacgattc gtcgcccatc gcggcggcgc ggcgagtcga
     7501 atcgacgata tcggtcaaca tgatggtcgc tgaaacgcga gcgccgggtc cggcctcgat
     7561 ccgatcgtcc gcccggtcgc cggcgagcgc aggcaagctc agcccggaaa gggcgagatc
     7621 gaccgcgatt ttcgaacgca ggcacgtgtc cgacaggttc aaaacgacgc ccatggccgg
     7681 gctcccattc cgcgatattc gtcttcatgc ctatgacgaa tgaggcgcgg cgttttggac
     7741 aggccgggcg atatttttcg atcgccaacc cttgattgag acaaatcgcc gccggcggcg
     7801 cgctaagccg agagtctcag ccagcccttg ggatccgcga aatgggaacg gtcttcgacc
     7861 aaagcgcggc agagatcggc catgttggcg agattgtgaa tgggacggaa ttcgtcgaca
     7921 tgcggcagca tggctctgat gccttgcgcc ttggcctcga acgcgccgta tcgcagcagc
     7981 ggattggccc agatcaatcg ccggcaggat ttgcgcaggc gctccatctc gcggccgagc
     8041 tcttgcggct cgtcgcgctc caacccgtcg gtgaacagga gcacgatcgc gccctgcccc
     8101 agcacgcggc
//
LOCUS       DATGJW010000079         5007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_428007, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000079 DATGJW010000000
VERSION     DATGJW010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5007
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428007"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..659
                     /locus_tag="VKU03_02385"
     CDS             <1..659
                     /locus_tag="VKU03_02385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SufD family Fe-S cluster assembly protein"
                     /protein_id="HLJ70136.1"
                     /translation="IKYSTVQNWYPGDAEGRGGIYNFVTKRAACRGRNSKVSWTQVET
                     GSAITWKYPSCILRGDNSRGEFYSIAISNGHQQVDSGTKMIHLGKNTTSRIISKGIAA
                     GQSQNTYRGQVSAHRLATGARNFTNCDSLLIGDKCGAHTVPYIEAKNSSATFEHEATT
                     SKISEDQLFYCQQRGLSGEEATAVIVNGFVRDVLQQLPMEFAVEAQKLIAISLEGSVG
                     "
     gene            902..1651
                     /gene="sufC"
                     /locus_tag="VKU03_02390"
     CDS             902..1651
                     /gene="sufC"
                     /locus_tag="VKU03_02390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HLJ70137.1"
                     /translation="MLEVKNLCVEIHGKPILDGLDLTVKAGEVAAIMGPNGSGKSTLS
                     YVIAGKADYEVTGGEILLDGENILELSPDERAAKGLFLAFQYPIEIPGVATMTFLKSA
                     LNALARQRGEKELTTADFMRRVREAANRLMIDQDMLKRPLNVGFSGGEKKRMEILQMA
                     LLQPRLAIMDETDSGLDIDALRIVAEGVNALRDPKRAFLVITHYQRLLNHIRPDAVHV
                     MARGKIVRSGGPDLALELERNGYRDYAADAA"
     gene            1659..2954
                     /gene="sufD"
                     /locus_tag="VKU03_02395"
     CDS             1659..2954
                     /gene="sufD"
                     /locus_tag="VKU03_02395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HLJ70138.1"
                     /translation="MTAHPAAARTAAESALAEQFSAGQGALPLPEREAAFRAFAAKGL
                     PSRRDEAWHYTDLRQRLTKVAPLAAAPGAAAIEAARARLSALPAGPARFVILDGRFAA
                     ALSSAPPEGVSVVSLGEDARASRAAAPDLGANVDAVLALNGALAQGGLAIKIASQTKL
                     AAPLEIRHWTSGGAAKSVASRVAIEIAAGARVAVAEYFEGSGARDQRNAATILEIGAG
                     AVVDHTAIVEGEAALHLESLVARLGEGAELASFGFVAGGDLARRQIFAKLVGKNAKIA
                     LCGLSLIGGKRHADTTLIVDHIAPYGESREFFKHIVADEATGVYQGKVIVEPGAQKTD
                     GGMKSQAILLSPTAVMNNKPELEIFADDVICGHGATVGALDREQLFYLRARGIPEAEA
                     EAMLLEAFGFEAIERVRGEALRDILRGKAKNWLAGRAKG"
     gene            2960..4204
                     /locus_tag="VKU03_02400"
     CDS             2960..4204
                     /locus_tag="VKU03_02400"
                     /EC_number="2.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_function: GO:0031071 - cysteine desulfurase activity
                     [Evidence IEA];
                     GO_process: GO:0006534 - cysteine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase"
                     /protein_id="HLJ70139.1"
                     /translation="MTIAQKIAAPYDVEAVRADFPILAERPYGKPLAYLDNAASAQKP
                     RQVLDRLRYAYEHEYSNVHRGLHYLANAATEGYEAARESVRRFLNAAAAEEVVFTRNA
                     TEAINLAAASFGLDHIREGDEIVLSVMEHHSNIVPWHFHRERKGAVLKWIDVADDGSF
                     SLQDFERALTKRTKIVAITQMSNVLGTAPPIKEIVRIAHSRGIPVLVDGSQGAVHLDV
                     DVRDLDVDFYAFTGHKVYGPTGIGALYGKREWLERLPPFNGGGEMIGEVTRDAITYNA
                     PPHRFEAGTPPIVQAIGLGAALDYVEGIGRARIRAHEDALRAYAHERLGAINALRLIG
                     HAEGKGPIVSFTMEGAHAHDVATIIDRFGVAVRAGTHCAMPLLARFGVTSTCRASFGL
                     YNTKAEIDALAEALEHARRLFA"
     gene            4220..4603
                     /locus_tag="VKU03_02405"
     CDS             4220..4603
                     /locus_tag="VKU03_02405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SUF system Fe-S cluster assembly protein"
                     /protein_id="HLJ70140.1"
                     /translation="MTDSPALEAQANKPAIETAPSIAPEELQRLTDDIVAALKTVYDP
                     EIPADIYELGLVYSIDIDDGRFVTIDMTLTAPACPVAGEMPGWVENAVGAVNGVSGVK
                     VNMVFDPPWDQSRMSDEARVALDMW"
     gene            complement(4624..>5007)
                     /locus_tag="VKU03_02410"
     CDS             complement(4624..>5007)
                     /locus_tag="VKU03_02410"
                     /inference="COORDINATES: protein motif:HMM:NF025731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="superinfection immunity protein"
                     /protein_id="HLJ70141.1"
                     /translation="RVQEARARPMLIVATLIYFLPTILALARGHLSTLAIFLLNLFLG
                     WTMIGWIVALIWSCTGFTAANYDRGEPQIIGPDGRIAARRANGSLWIVLILILVAIAI
                     LDKHRHFRDLRSFSNPFPMENGLRL"
BASE COUNT          931 a         1622 c         1627 g          827 t
ORIGIN      
        1 agatcaagta ctcgacggtg cagaactggt acccgggcga cgccgagggc cgcggcggca
       61 tctacaattt cgtgaccaag cgcgcggcct gccgcggccg caactccaag gtctcgtgga
      121 cccaggtcga gaccggctcg gcgatcacct ggaaataccc gtcctgcatc ctgcgcggcg
      181 acaattcgcg cggcgaattc tactcgatcg ccatctcgaa cggccatcag caggtcgaca
      241 gcggcaccaa gatgatccat ctgggcaaga acacgacgag ccggatcatc tccaagggca
      301 tcgccgccgg ccagtcgcag aacacctatc gcggccaggt ctcggcgcat cgcctggcga
      361 ccggcgcgcg caatttcacc aattgcgatt cgttgctgat cggcgacaaa tgcggcgcgc
      421 atacggtgcc ctatatcgag gcgaagaatt cgtcggcgac gttcgagcac gaggcgacga
      481 cctcgaaaat ctccgaggac cagctcttct actgccagca gcgcggcctc tcgggcgaag
      541 aggccaccgc ggtcatcgtc aacggcttcg tccgcgacgt cctgcagcaa ttgccgatgg
      601 agttcgcggt cgaagcgcag aagctgatcg cgatctcgct tgaggggagc gtcgggtgat
      661 cggacaggcg gagcaaattt tgtccgacgt agaaggactt cgacgccttt ggcgcgaggg
      721 gcttgagagc ggacttggtc gaacatgtcg attgaagaga tcaagcgcga ggcgcgccgc
      781 ggcatggtca ggcggaaagt atagaccggc caaacggtcc gccgactgat ttgaggcgcg
      841 gacgcggttt gcaagtgttg gcggcagcca aagacccaaa gaatttgaga gaagatgatt
      901 gatgcttgaa gtcaaaaacc tctgtgtcga aatccacggc aagcccatcc tcgacggcct
      961 cgacctcacc gtgaaagccg gcgaggtcgc cgcgattatg gggccgaacg gctcgggcaa
     1021 gtcgacgctg tcctatgtca tcgccggcaa ggcggattac gaagtcaccg gcggcgaaat
     1081 cctgcttgac ggcgagaaca ttctcgaact ttcgccagac gagcgcgccg cgaaaggctt
     1141 gttcctggcc tttcagtacc cgatcgagat tcccggcgtc gccacgatga ccttcctgaa
     1201 atcggcgctg aacgcgctcg cccgccagcg cggcgagaag gagctgacca cggccgattt
     1261 catgcgccgc gtgcgcgaag ccgccaatcg cctgatgatc gaccaggaca tgctcaagcg
     1321 cccgctcaat gtcggcttct cgggcggcga aaagaagcgc atggagattt tgcagatggc
     1381 gctgctccag ccgcgcctcg ccatcatgga cgagacggat tccggtctcg acatcgacgc
     1441 gttgcgcatc gtcgccgagg gcgtcaacgc cctgcgcgat ccgaagcgcg cctttctcgt
     1501 catcactcac tatcagcgcc tgctcaatca cattcggccg gacgcggtgc atgtgatggc
     1561 gcgcggcaag atcgtccgca gcggcggacc ggatctggcg ctcgaactgg agcgcaacgg
     1621 ctaccgcgat tatgccgcgg acgccgcgtg aggcggtcat gacagcccat cccgccgccg
     1681 cacgcaccgc tgctgaaagc gcgctcgccg agcaattctc cgccgggcag ggcgccttgc
     1741 cgctgcccga gcgcgaggcg gcgttccgcg ccttcgcggc caagggcctg ccgagccgcc
     1801 gcgacgaagc ctggcattat accgatttgc gccagcgcct gaccaaggtc gctccgctcg
     1861 ccgccgcgcc cggcgccgcg gcgatcgagg ctgcgcgagc gcgtctatcc gcgcttccgg
     1921 ccggccccgc gcgcttcgtc attctcgacg gccgctttgc ggcggctctg tcttccgcgc
     1981 cgcccgaagg cgtgagcgtc gtctcgctcg gcgaggacgc gcgggcgagc cgcgccgccg
     2041 cgcccgatct cggcgccaat gtcgacgccg tgctcgccct caatggcgcg ctggcgcagg
     2101 gcggcctggc gatcaagatc gcgtcgcaaa cgaaattggc cgcgccgctc gaaatccgtc
     2161 attggaccag cggcggcgcg gcgaaatcgg tcgcctcgcg cgtcgcgatc gagatcgcgg
     2221 cgggggcgcg cgtcgcggtg gccgaatatt tcgaaggctc cggcgcccgc gatcagcgca
     2281 atgcggcgac gattctcgag atcggcgccg gcgctgttgt cgaccatacc gcaattgtcg
     2341 agggcgaggc ggcccttcat ctcgaaagcc tagtcgcgcg actcggcgag ggcgccgagc
     2401 tggcctcgtt cggcttcgtc gccggcggcg acctggcgcg tcggcagata ttcgcgaaac
     2461 ttgtcggcaa gaacgccaag atcgccttat gcggtctgtc gctcatcggc ggcaaacgcc
     2521 acgccgatac gacgctcatc gtcgatcaca tcgcgccgta tggcgagagc cgcgaattct
     2581 tcaagcatat cgtcgcggac gaggcgaccg gcgtctatca gggcaaggtc attgtcgagc
     2641 ccggcgcgca aaagaccgat ggcggcatga agagccaggc gatcctgctt tcgcccacgg
     2701 ccgtcatgaa caacaagccg gagctcgaaa tcttcgccga cgacgtcatt tgcggccatg
     2761 gcgcgaccgt cggcgcgctc gaccgcgagc aactattcta tctgcgcgcg cgcggcatcc
     2821 ccgaagctga ggccgaggcc atgctgctcg aagccttcgg cttcgaggcg atcgagcgcg
     2881 tcaggggcga agcgctgcgc gacatattgc gtggcaaggc gaaaaactgg ctggccggcc
     2941 gcgcgaaagg ttgaacgcca tgaccatcgc ccagaaaatc gcggcgcctt acgatgtcga
     3001 ggcggtgcgg gctgatttcc cgatccttgc agagcggccc tatggcaagc cgctcgctta
     3061 tctcgacaac gccgcctcgg cgcaaaagcc gcgccaggtt ttggatcggc tgcgctacgc
     3121 ctacgagcac gaatattcca atgtgcaccg cggcctgcac tatctcgcca acgccgcgac
     3181 cgagggctac gaggcggcgc gcgaaagcgt gcggcggttc ttgaacgcgg ctgcagccga
     3241 agaggtcgtt tttacccgca acgccaccga ggcgatcaat ctcgccgccg ccagtttcgg
     3301 tctcgatcat atccgcgaag gcgacgagat cgtcctttcg gtcatggagc accactccaa
     3361 cattgtgccg tggcacttcc atcgcgagcg aaagggcgcc gtgctgaaat ggatcgatgt
     3421 cgccgacgac ggctccttct cgttgcagga tttcgagcgc gcgctgacga aacgtacgaa
     3481 aatcgtcgcg atcacccaga tgtccaacgt gctcggcacg gcgccgccga tcaaggagat
     3541 cgtccgcata gcccatagcc gagggattcc ggtcctggtc gacggctccc agggcgcggt
     3601 tcatctcgat gtcgacgtgc gcgatctcga cgtcgatttc tacgccttta ccggccacaa
     3661 ggtttatggc ccgactggga ttggcgcgct ctacggaaag cgcgaatggc tcgaaaggct
     3721 gccgcctttc aacggcggcg gcgagatgat cggcgaggtc acgcgcgacg ccatcaccta
     3781 caatgcgccg ccgcatcgtt tcgaggccgg cacgccgccg atcgttcagg cgatcgggct
     3841 cggcgcggcg ctcgactatg tcgagggaat cgggcgcgcc cggatcaggg cgcacgagga
     3901 cgcgttgcgc gcctacgcgc atgagcggct gggcgcgatc aatgcgctgc gcctgatcgg
     3961 ccatgccgag ggcaagggac cgatcgtctc gttcaccatg gaaggcgcgc acgcccatga
     4021 cgtcgcgacg atcatcgacc ggttcggcgt cgcggtgcgg gccggcaccc attgcgcgat
     4081 gccgctgctc gcccgtttcg gcgtgacctc gacttgccgc gcctcgttcg gcctttacaa
     4141 taccaaagcg gaaatcgacg cgctggccga ggcgctcgag cacgcgcggc gtctcttcgc
     4201 ctgaaggagt cttggcgtca tgaccgacag tccggccctc gaggcccagg ccaacaagcc
     4261 ggcgatcgaa acggcgccgt cgattgcgcc cgaagagctg cagcgcctga ccgacgacat
     4321 cgtggcggcg ctgaagacag tctacgatcc ggaaattccg gccgacattt acgagctcgg
     4381 cctcgtctat tcgatcgata tcgacgacgg acggttcgtg acgatcgaca tgaccttgac
     4441 cgcgccggcc tgtcccgtgg ccggcgaaat gccgggctgg gtcgagaacg cggtcggcgc
     4501 cgtcaatggc gtttccgggg tcaaggtcaa catggtcttc gatccgccct gggatcagag
     4561 ccgcatgtcc gacgaggcgc gggtcgcgct cgatatgtgg tgaagagtcc gcccgtccgc
     4621 gcctcagaga cgcaggccgt tctccatcgg gaagggattt gagaacgagc gcaggtcgcg
     4681 gaaatggcgg tgcttgtcga gaatggcgat cgccaccagg atcagaatga gaacgatcca
     4741 cagcgagcca ttggcgcgcc gcgcggcgat ccggccgtcc gggccgataa tctgaggctc
     4801 gccgcggtcg taattggccg cggtgaagcc ggtgcatgac cagatcaggg cgacgatcca
     4861 gccgatcatc gtccagccca ggaacagatt cagcaggaaa atcgccaggg tcgacaagtg
     4921 tccgcgcgcc agagcaagga tggtcggcag gaaatagatc agcgttgcga cgatcagcat
     4981 tgggcgggcc ctggcttctt gaacgcg
//
LOCUS       DATGJW010000080        14470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_428250, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000080 DATGJW010000000
VERSION     DATGJW010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 14470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14470
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_428250"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            62..946
                     /locus_tag="VKU03_02415"
     CDS             62..946
                     /locus_tag="VKU03_02415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase"
                     /protein_id="HLJ70142.1"
                     /translation="MTRRLVLSDGVAEAVVLPRLGAGLERYDLISRGRREPLFRAAPT
                     GASEPFALANILLAPWSNRISGGGFHFGGAFHRLEANVDGEPFPIHGNAFLSHWRIKE
                     EGRAHAMLLLSSDGPGPFRYDAEMTYALSGGALTMRLIIINRAAISLPYGLGFHPWLP
                     RTPDVLLTAPAQGAWLEDERHLPAGRLATAERPEWDFSKPRPLPAQWINNAFDGWRGS
                     AVVSWPSRLLSLAIEAGPGLGVYIVYSPNADADFFCFEPVSHSVDAHNLGRSASGLAV
                     LAPGEKAEASCRFVPQSA"
     gene            1075..3450
                     /locus_tag="VKU03_02420"
     CDS             1075..3450
                     /locus_tag="VKU03_02420"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLJ70143.1"
                     /translation="MKTRKIASKARKALEKPQTTGWKTSDEDELSVRRWRGRTELAAI
                     EALEPAYGIFGAFRARSHTGGVYEVEIRDLVGRDNSCGCIDHRVNGLGACKHIEGALA
                     ALRRKYGARAFKAAAEQGSPRAEIFIARQGHARPTLNRPHVGLNPSAEDWLAPFLDAK
                     GELRTDSRTIAAVIAAAPAAPPAIRVSRHMTPFLERARRLAGREIDKARFRRALETGE
                     ASWNILKSPLLPYQKEGALHLAFGERALLADEMGLGKTIQAIAACEILARRKKIARVL
                     VVSPTSVKAEWEDQIARFTGRAALFVAGLRPERLKRYAEPAFFTLVNYEQVVRDAEDI
                     NRLLKPDVVILDEAQRIKNWQTKTARQVKSLKSPYAFVLTGTPLENRIDETYSIVEYL
                     DPEIFGSLFRFNRDFYELDERGRPTGYRNLDELHRRLSGIMLRRRKKDVEDELPGRTI
                     KTFFVAMAEEQAVRYGEYELRARRLAALAERRPLMKEEFDRLQQYLACMRMICDTPAI
                     LDPECRVSPKLEELEGVLAELLEEEDRKIVIFSEWVRMLDLVRGLAREMGIEHAWHTG
                     DVPQLARRAEIARFRADRSCRLFLTSDAGATGLNLQVANCVINMDLPWNPAKLEQRIA
                     RAWRKGQISPVTVINFVTENSIEHSIFHLLTHKQTLADGVLDGTDDFSAVKMPSGRAA
                     LIERMEAMLGVKATPVKVLSPEEATVEEMRTRHGARLHHAEARAGRLLLILDADAEGI
                     AAEGARFSAGEPAVEILDPATWKTLQRLAASGLVNFAAPPGRVLHSAVGAE"
     gene            complement(3467..4465)
                     /locus_tag="VKU03_02425"
     CDS             complement(3467..4465)
                     /locus_tag="VKU03_02425"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ70144.1"
                     /translation="MTLQVGLIGCGVMGSDHARILQNEVAGARLVAAYDAQAARAETV
                     AASAKARTLPSARALIADPEVQAVIIASPDETHAELSIACIEAGKPALCEKPLAATLD
                     QCRAVMAAEMKAGRRLVQVGFMRRFDPGYRAIKGKIGDSGLGRPIFLHCVHRNAIAPS
                     YITSDLVLANSAVHEFDISRFVLDEEFATIQVVSGKATRHAETRRPQFVVLESRSGVV
                     VTIEVFLDAQYGYDVQAELVCENGSASLSPHRPIAQRLAGRDGFEVESDWRQRFADAY
                     RLQLQEWVAAIAAGRAVGASAWDGYAASAIAAAALEAAAKRAATKIGIGERSAFYG"
     gene            complement(4498..5559)
                     /locus_tag="VKU03_02430"
     CDS             complement(4498..5559)
                     /locus_tag="VKU03_02430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015915619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HLJ70145.1"
                     /translation="MSFSQTIAPAKVVRAAEGATDYKAVRLWLFTVAALIFVMVLVGG
                     ATRLTESGLSITEWKPVTGVIPPLNGADWQAAFSAYKQIPQYSQMFPDMTLSEFKTIY
                     FWEWSHRLLARIIGAAFILPLLWFWAKGRLRGIGLLMLLPTGLLALEPIVGWWMVASG
                     LSRRVEVSQVRLAIHLLIATATYGALLWIAVGLSERRREAATSGLKWASGLIACLVFC
                     QIGFGALVAGLRAGLIDNTWPLMEGRLIPADLWPQSPWWSNLYQDAASAQFQHRILAY
                     AIVLLALWHAVAAMRRLPGSSVARRAAAVAGLALSQAAIGIATLLLVVPIGAGLLHQA
                     FAMLLFGMAVAHWRATALG"
     gene            5663..5872
                     /locus_tag="VKU03_02435"
     CDS             5663..5872
                     /locus_tag="VKU03_02435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2842 domain-containing protein"
                     /protein_id="HLJ70146.1"
                     /translation="MNNRARKLIGGAGMLAFVLVYALVAMALAQSRPIQEAPALAQTV
                     CYAILGLAWTLPLMPLIKWMERRNP"
     gene            complement(6383..7435)
                     /locus_tag="VKU03_02440"
     CDS             complement(6383..7435)
                     /locus_tag="VKU03_02440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLJ70147.1"
                     /translation="MALKDKVIRLGFTSFRATGLHRLASPLTRGRGVILMLHHVRPWR
                     PPTPGFEPNRGLEITPEFLDATLKTARRAGFEFVTMDEALRRLTQGGAPFAALTFDDG
                     YRDLRDFALPILERWQAPFVAYIATGYAGRSARLWWLELEEAVRRLDRIEIAAPDFAA
                     ALPSASEKEKAAAFDRLYWRLRDGDEERLLRVIGDLVAQAKVDGAAMVEQLCFDWAEI
                     AELSRHPLATIGAHTVHHRMLAKWPEEAARDEMAASKAELESALGREIRHFAYPVGDP
                     TSAGRREFALARELGFASAVTTRPGMLFEEHRGHENALPRLSISGAWQDVGYLEVLLS
                     GAPFALWNRGRRLNVA"
     gene            7555..8763
                     /locus_tag="VKU03_02445"
     CDS             7555..8763
                     /locus_tag="VKU03_02445"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ70148.1"
                     /translation="MSQAQNLATGAMGSASARECAAPARAFAEFEIRDDPQAASAAWD
                     EIHADGLATAYQSKAFVGLWLRTVGRAEAVEPMIVLVRDERGGISALLPLAVRWRLGL
                     RVAEFVGGKHANFHMGVFRAGLAVDRDEVLGLLRRIGASARLDAFFFVNQPELWQGSS
                     NPLRALGGQKSPSFGHATQLQKNFDQWFEAHYSKAARRKLRKKTRRLAERGAVASFVA
                     QDEASAREALAALFAHKAARAKATGQVNDFDDPAAVRFLEIAACERAAEHGALIELRA
                     MRCGERIVAVFGGLAHAGRFYGVVTSHDPDAEIARSSPGELLILDVIRDLHERGFKTF
                     DLGVGEARYKDAVCETSEALFDSALGLTFKGRAASLCFLLARRVKRWIKQRPWAWSLV
                     APMRRRLRPS"
     gene            complement(8773..10911)
                     /locus_tag="VKU03_02450"
     CDS             complement(8773..10911)
                     /locus_tag="VKU03_02450"
                     /inference="COORDINATES: protein motif:HMM:NF014732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exopolysaccharide transport family protein"
                     /protein_id="HLJ70149.1"
                     /translation="MSNQRTERAEAGGDAIDLPKLWRSVRRRKSWIIVPTALAFAASL
                     AVIALVHPRYTGVAKVLLVNQESYFTRPDKAPQEATTAPDPEAVQSQAEAIMSTALAR
                     KAIVKLDLANRPEFSRAAAPGVAESLLSLIGLTRQPPPGRTAEDRLVDTFLSHLNAFP
                     VAKTRVLQIEFVSQDAEFAARGANVVAQLFLDQQEEAKKDAAKAASAWLAAKITELRA
                     KVADADGKVEAFRAQSGLLSTGNSLTAPSQQLADINAQLAAARSAQATSQAKAQMLRS
                     LARNGRLDEDPDVARDDALRRLTEQRATLKAQIAQESRTLLPEHPRMKALSAQLAEID
                     SQIRLAAEKAARALENEARGAGDRVKALQETLAAQAKTVAAGNQEDVQLRALQLDANA
                     ARDQLESYVQKYREAIARDADNAAPADARVIAPATEPRYPTFPKKIPTLLLGALAGFL
                     LSAGIVVSRALIEDGEEAPVVRRPVAAARIEPERIRRPDEQAAGVPPPPLPARASESV
                     DQDDASGSAGQIAGLGELIAHVDRQGRTGEPRFALIAGCGDGASRGMALELGRALAAK
                     GRAVLVDLGPGPQNWLSEISPPEGDRLVGLSEVLAGEASFAAALRRDASSRLDIMQAG
                     DASASVGDALDEALVALAETYDFVVAHAGDWRDEAAQVAAEAIREMIIVAPQTVLPSA
                     IAEARGQIGAACVVIGCGAAGRRSRLERAA"
     gene            11083..11643
                     /locus_tag="VKU03_02455"
     CDS             11083..11643
                     /locus_tag="VKU03_02455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009468197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HLJ70150.1"
                     /translation="MRSFHFAFILIVLGALTGCAARPYNAEFFRAQINAPYQLASGDR
                     VRVIVFGQDSLSNSFSVDPAGNISMPLIGLVKAYGLTTAELEREIAARLRNGYIRDPR
                     VSVEVEAFRPFFVLGEVQTAGQYPYVSGLTIQSAVAIAGGFSPRGYQAGADVTRMVNG
                     RLITAWEPLTFPVRPGDTLTIRERFF"
     gene            11692..12897
                     /locus_tag="VKU03_02460"
     CDS             11692..12897
                     /locus_tag="VKU03_02460"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLJ70151.1"
                     /translation="MSAARSFDAQKLRIVHVFRAPLGGLFRHVIDLACEQAARGHDVG
                     LFFDSEFRGDNVAAALARLQGGLRLGAHGCPIKRNPHPGDIGAMLKFSALLRRLRPDI
                     VHGHGSKGGLYARLPGFFARPDGPARAYTPHGGSFNFKPGTALHRLFMTTEKALSFAT
                     DVFLFESAYIARRYDSYVGVDHGIRRVVANGVGPAEFSPIEPAPDAADLLYVGELRAA
                     KGIDTLLEAIALAEARFGKSWRAVLVGSGPDKEMLLGQAEKLGVARQISFVGPLPARQ
                     AFRLGRILVAPSRAESLPYIILEAAGAQLPIVATNVGGIPEIFGPYADRLGPCDDPAD
                     LAARILAAMDLSEADRKARCAELAAHVAANFSIKTMVDSVLEGYRAALARRLPRQAGE
                     AVAKVAPTA"
     gene            12903..14432
                     /locus_tag="VKU03_02465"
     CDS             12903..14432
                     /locus_tag="VKU03_02465"
                     /EC_number="2.7.8.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265106.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-phosphate glucose
                     phosphotransferase"
                     /protein_id="HLJ70152.1"
                     /translation="MNAFSPKKLRAVPNPASPRPPQAAASRPKRLEELAAEPTKPRYS
                     SVVLAGLVRAAEFILIVISATLVHRLYVAPSVGYAPSYFIIAAAVAFLSMVVFQGLQT
                     YHPTAFRQPIRQVFRIAGGWSFVFLIVFAAIFFLKLDGVISRVWVATWFLLGLAALAV
                     ERALLSRIALAMVRAGRLDRRTVVVGGGEFGRDLLRDLAKARHSEIKMLGVFDDRGDD
                     RAPETVEGFAKLGNVDDLVEFARHTRIDLVIFALPITAEQRILEMLRKLWVLPIDIRL
                     AAHANRLRFRPRSYSYVGHVPVLDILDKPIADWDVVIKLAFDKIVGGLALIALSPILL
                     ATALAVKLDSPGPVLFRQKRYGFNNEMIEVFKFRSMYVDQTDNNAAKLVTKDDPRVTR
                     VGRFIRKTSLDELPQLFNVVFKGDLSLVGPRPHAVHAKAADRQYDEVVDGYFARHRVK
                     PGITGWAQINGWRGETDTPEKIQQRVEHDLYYIENWSIALDLSILARTPFALLRTKNA
                     Y"
BASE COUNT         2471 a         4887 c         4589 g         2523 t
ORIGIN      
        1 tcctcgtctc gacggcctcg gacatgatca ctgggcagaa cttcgcaatc gacggaggct
       61 ggtgacgcgg cgccttgttc tttccgacgg cgtcgccgag gcggtggttc taccccgcct
      121 cggcgccgga cttgaacgtt acgacctgat ctcgcggggc cggcgcgagc ccttgttccg
      181 cgccgcgccg acgggagcga gcgagccttt cgcgctcgcc aatattttgc tcgcgccctg
      241 gtccaaccgc atctcgggcg gaggctttca tttcggcggc gcgtttcatc ggctcgaagc
      301 caacgtcgac ggcgagccct tccccatcca tggcaacgcc ttcttgtcgc attggcggat
      361 caaggaggag gggcgcgcgc acgcaatgct gcttctgtcg agcgacggcc ccggcccgtt
      421 ccggtacgac gctgagatga cctacgcgct ttcgggcggc gcgctcacga tgaggctgat
      481 catcatcaac cgcgcggcga tttcgttgcc ctatggtctt ggctttcatc cctggctgcc
      541 gcggacgccg gacgttcttc tcacggcgcc ggcgcaaggc gcgtggctcg aagacgagcg
      601 tcatttgccg gccggccggc tcgcgaccgc cgagcgcccc gaatgggact tctccaaacc
      661 gcgtcctctg ccggcgcaat ggatcaacaa cgccttcgac ggttggcgcg gaagcgccgt
      721 cgtttcctgg ccgagccggc tgctctcgct ggcgatcgag gcgggaccgg gcttgggcgt
      781 ctatatcgtt tattctccca acgccgatgc cgactttttc tgcttcgagc ctgtcagcca
      841 ttcggtcgac gcgcataatc tggggcgcag cgccagcggg ctcgcagtct tggcgccggg
      901 cgagaaggcc gaagcgtcgt gccgtttcgt cccgcagagc gcctgagcca ggccgtttac
      961 gcggcctgct ccattgctgt gaatattccg atctttcggt tcgccgcggc aagcgcctcg
     1021 cgataagagc gatcatcgtt tctgagccga ttcagggccg cttgcgcaat gccggtgaaa
     1081 accaggaaaa tcgcctccaa agcgcgcaag gcgctcgaaa agccgcaaac aacgggatgg
     1141 aaaaccagcg acgaagacga attgagcgtt cggcgctggc gcgggcgcac cgagctcgcc
     1201 gcaatcgagg cgctggagcc tgcttatgga attttcggcg ccttccgcgc ccgctcccat
     1261 accggcggcg tttatgaggt cgagattcgc gaccttgtcg gccgcgacaa ttcctgcggc
     1321 tgcatcgatc atcgcgtcaa cgggctgggc gcctgcaaac atattgaagg cgcgctcgcc
     1381 gccctgcgcc ggaaatatgg agcccgggcc ttcaaggccg ctgccgagca aggatcgccg
     1441 cgcgccgaaa ttttcatcgc ccgacaaggc cacgcccgtc cgacgctcaa tcggccgcat
     1501 gttgggctca atccatcggc cgaggattgg ctggcgccct ttctcgacgc gaaaggcgag
     1561 ctcagaaccg attccaggac aatcgcggcc gtgatcgcgg cggcgcccgc cgctccaccg
     1621 gcgattcgcg tctcgcggca tatgacccct tttctggagc gcgctcgccg cctcgccggc
     1681 cgcgagatcg acaaggcgcg gttccgccgc gcgctggaga ccggcgaggc ctcctggaac
     1741 atcctcaagt cgccgctcct gccctaccaa aaagagggcg cgcttcatct cgcctttggc
     1801 gagcgcgccc tgctcgccga cgagatgggc ctcggcaaaa ccatccaagc catcgctgct
     1861 tgcgaaatcc ttgcgcgccg gaaaaagatc gcgcgcgtgc tggtggtttc gccgacctcg
     1921 gtcaaagcgg agtgggagga tcagatcgcc cgcttcaccg gccgagccgc gcttttcgtc
     1981 gcgggcctgc ggccggaacg gctcaagcgt tacgccgaac cggccttttt cacgctggtc
     2041 aattacgagc aggtcgtcag agacgccgag gacatcaacc ggctgctcaa gcctgatgtg
     2101 gtgatcctcg acgaagccca gcgcatcaag aattggcaga ccaagaccgc gcggcaggtc
     2161 aaatcgctga aatcgcccta cgcgttcgtt ttgactggga cgccgctcga aaaccgcatc
     2221 gacgagacct attcgatcgt cgaatatctc gacccggaaa ttttcggctc gctgttccgc
     2281 ttcaaccggg acttctacga gctcgacgag cgcggccgcc cgaccggcta ccgcaatctc
     2341 gacgaactgc accgccggct ttccggaatc atgctgcgtc ggcgcaagaa ggatgtggaa
     2401 gacgagctcc cgggccgtac gatcaagacc ttttttgtcg ccatggcgga ggagcaagcc
     2461 gtccgctatg gcgaatacga gctcagggcc agaaggttgg ccgcgctcgc cgagcgccgg
     2521 ccgctgatga aagaagaatt cgaccgattg cagcaatatc tcgcctgcat gcgcatgatt
     2581 tgcgacacgc ccgccattct cgaccccgaa tgccgcgtct cgccaaagct ggaagaactc
     2641 gaaggcgtcc tcgcggagct cctcgaagaa gaagatcgca aaatcgtcat tttctccgaa
     2701 tgggtgcgca tgctggattt agtgcgcggc ctcgcccgcg agatgggaat cgagcatgcc
     2761 tggcacaccg gcgacgtgcc gcaactcgcc cgccgcgccg agatcgcgcg cttccgcgcc
     2821 gaccgctcat gccggctgtt cctaacctcc gacgccggcg cgacgggact caatctgcag
     2881 gtcgccaatt gcgtcatcaa catggacttg ccctggaatc cggcgaagct tgaacagcgc
     2941 atcgcccgcg cgtggcgcaa ggggcaaatc agcccggtca cagtcatcaa tttcgtcacc
     3001 gaaaactcga tcgaacattc gatcttccac ctcctgactc acaagcaaac gctcgctgac
     3061 ggcgtgctcg acgggacgga tgatttctcc gcggtcaaga tgccttccgg ccgcgccgcg
     3121 ctgatcgagc gcatggaggc gatgctgggc gtcaaggcga cgccagtcaa agttctttcg
     3181 ccggaagagg cgacggttga ggagatgagg acacggcacg gcgcacgcct tcatcacgcg
     3241 gaagcgcgcg cggggcgctt gctcctcata ttggacgccg acgccgaagg catcgccgcc
     3301 gagggcgcac gcttttccgc cggagaaccc gcagtcgaga tcctcgaccc ggcgacctgg
     3361 aagacgctgc agcgcctcgc cgcctccggc ttggtgaatt tcgccgcgcc gccaggacgc
     3421 gtgcttcatt cggcggtcgg cgccgaatga atcctctccg cctcggctag ccgtaaaagg
     3481 ccgaccgctc gccgatcccg atcttggtcg ccgcccgctt cgccgctgcc tccaaagccg
     3541 cggcggcgat ggcggatgcg gcgtagccgt cccaggcgct ggcgccgacg gcgcggccag
     3601 cggcgatggc cgcgacccat tcttgaagct gaaggcggta ggcgtcggcg aagcgctggc
     3661 gccaatcgct ctcgacctcg aaaccgtcgc ggccggcgag cctttgcgcg atcggccgat
     3721 gcggcgacag agaggcggag ccgttctcgc aaaccagttc ggcctgaacg tcgtagccat
     3781 attgcgcgtc gagaaagacc tcgatcgtca cgacgacgcc gctccgcgat tccaggacga
     3841 cgaattgcgg ccgccgcgtc tcggcgtgtc gcgtcgcttt gccgctgacg acctggatcg
     3901 tcgcgaactc ctcgtcgagg acgaagcgcg aaatgtcgaa ttcgtgaacc gccgaattgg
     3961 cgagaaccag atcggaggtg atgtagctcg gcgcgatcgc gttgcgatgg acgcagtgga
     4021 ggaagatcgg ccggccgagc ccggaatcgc cgattttgcc tttgatggcg cggtagccgg
     4081 gatcgaaccg ccgcatgaag ccgacctgga cgagccgccg gccggctttc atctccgccg
     4141 ccatcacggc gcggcattga tcgagggtcg ccgcgagcgg cttctcgcaa agcgcgggct
     4201 tgccggcttc gatgcaggcg atcgaaagct cggcatgggt ctcgtcgggc gaggcgatga
     4261 tcaccgcctg cacttcaggg tcggcgatca aggcgcgggc cgagggcagc gtccgcgcct
     4321 tggcgctcgc ggcgaccgtc tcggcgcgcg ccgcttgcgc gtcataagcc gcgacgaggc
     4381 gggcgccggc gacctcgttt tgcaggatgc gggcgtgatc gctccccatc acgccgcaac
     4441 cgatcaaccc gacctgcaat gtcattcgcc cctccccgaa aaacgtcccg gccgcgctta
     4501 gccgagcgcg gtcgcgcgcc aatgcgcgac cgccatgccg aacagcagca tggcgaaagc
     4561 ctgatgcagc agccccgccc cgatcggcac gacgagaagc agcgtggcga ttccgatcgc
     4621 ggcttgagag agcgcgagcc cggccacggc ggcggcgcgg cgcgcgacgg agctcccggg
     4681 aagccggcgc atcgccgcga cggcgtgcca cagggcgagg aggacgatgg cgtaagccag
     4741 aatccggtgc tggaactggg cgctggccgc gtcctgatag agattgctcc accacggcga
     4801 ttgcggccag agatcggcgg ggatcagccg gccctccatc agcggccagg tattgtcgat
     4861 cagcccggcg cgcagaccgg cgaccagcgc gccgaagccg atctggcaga agaccaggca
     4921 ggcgatcagg ccgctcgccc atttgagccc gcttgtcgcc gcctcgcgcc gccgctcgct
     4981 caggccgacc gcgatccaga gcagcgcgcc gtaagtcgcc gtcgcgatca agagatggat
     5041 cgccagccgg acttgcgaca cctcgacgcg gcggctgagc ccggaggcga ccatccacca
     5101 gccgacgatc ggctccagcg cgagcaagcc ggtcggcagg agcatgagaa ggccgatccc
     5161 gcgcaagcgg cccttcgccc agaaccagag gagcggcagg atgaaagcgg cgccgatgat
     5221 ccgcgccagc agcctatggc tccactccca gaaatagatg gttttgaatt cggataaagt
     5281 catatccgga aacatctggg aatactgcgg aatctgctta taggcggaga acgcggcttg
     5341 ccagtcggcg ccgttcaacg gcgggataac gccggtcacc ggcttccatt cggtgatcga
     5401 caagccggat tcagtgagcc gggtcgcgcc gccgaccaac accataacga aaatcagcgc
     5461 cgcgaccgta aagagccaaa ggcgaacagc cttgtaatcc gtcgcgccct cggcggcgcg
     5521 cacgacttta gcgggcgcga tggtctggct gaacgacatg gcctctccga aactcgcctt
     5581 gaaaccattg ggcggcggag acataaacgc ggccgtgcgg ccggacaatg gccgcctcgt
     5641 cgcagcctcg gaggccgtct tcttgaacaa tcgcgcgcga aaactaatcg gcggcgccgg
     5701 gatgctggcc tttgtgcttg tctatgcgct ggtcgccatg gcgctggcgc aatcgcggcc
     5761 gatccaggaa gcccccgcgc tggcacagac cgtttgctac gcgattctcg gccttgcctg
     5821 gacgctgccg ctcatgccgc tgatcaagtg gatggagcgg cgcaatcctt aaggtgatga
     5881 cccaagctgt cgcgcgagca ggccatcagg caaatgtctg cttaatcatc tgtgcgaagt
     5941 cgtggagcca actccgagtc gttggattac tggacaacag caaatatgta ccgacgacaa
     6001 atccaataat catgccaacg gataagcaat cgccaactat aacaattctc ctacaattta
     6061 gaccagcgta tcctctaatc aagatgtatt ggataagctt cagttgatat ccaggtggcg
     6121 ttggcttgaa aaacaaaggg ccgcccaacg ccttccaagt tgaattgtcg taacgcataa
     6181 ggtctctaac aaactgaccg taggctaccg ccaagaatac aagtaagaat atgaatagag
     6241 aagcaagtaa acgatcaatg tttgtcagat ccattggcgc gctccgccgc aagctaaccc
     6301 agtagagaga gcacgacgta gcgcgccggt agcgcctcct cctaccatgg atagttgtgt
     6361 cggcgtcccg agtaattcta acttaagcga cattgagccg ccgcccgcgg ttccataggg
     6421 cgaagggcgc gcccgacaaa aggacttcga gatagccgac atcctgccag gcgccgctga
     6481 tcgacaggcg cggcagagcg ttctcgtgac cgcgatgctc ctcgaaaagc atgcccggcc
     6541 gcgtcgtcac cgcgctggcg aagccgagct cgcgggcaag cgcgaattcg cgccgcccgg
     6601 cgctggtcgg atcgcccacc gggtaggcga agtggcggat ttcgcggccg agcgcgcttt
     6661 cgagctcggc tttggacgcc gccatctcgt cccgcgcggc ctcctccggc catttggcga
     6721 gcatgcgatg atggaccgtg tgagcgccga tcgtcgccaa ggggtggcgc gacaattcgg
     6781 cgatctcggc ccaatcgaag cacaattgct cgaccatcgc cgcgccgtcg actttcgcct
     6841 gcgcgacaag gtcgccgatc acgcgcagca gccgctcctc gtcgccgtcg cgcaagcgcc
     6901 aatagaggcg atcgaaggcg gcggcctttt ccttctcgct cgcgctgggc agcgcggcgg
     6961 cgaaatccgg cgcggcgatc tcgatccggt cgaggcggcg cacggcctcc tccaattcca
     7021 gccaccagag ccgcgccgag cgcccggcat aaccggtcgc gatataggcg acgaagggcg
     7081 cctgccagcg ctccaggatc ggcaaggcga aatcgcgcag atcgcgatag ccgtcgtcga
     7141 acgtgagcgc ggcgaaaggc gcgccgccct gggtcaagcg ccgcagcgcc tcatccatcg
     7201 tgacgaattc gaagccggcg cggcgcgccg ttttcagcgt cgcatcgagg aattccggcg
     7261 tgatctccaa gccgcggttc ggctcgaaac ccggcgtcgg cggccgccag gggcggacgt
     7321 gatgcaacat caagatcacg ccgcggcctc gcgtgagcgg gctcgccagg cgatgcaggc
     7381 cggtcgcgcg aaacgacgta aagccgaggc ggataacttt gtctttgagc gccatgaggt
     7441 tgcgccagcc tagcagagcg ggcttgccaa gtgcttaccc gctcttgcca tcggcaagat
     7501 tgcgttgacc aatttgtgcg agcgtccggc tttcggccga aagaaagcag ggctttgagc
     7561 caagcgcaaa acctcgcgac cggcgcgatg ggttcagcct cggcgcgaga atgcgcagcg
     7621 cccgcgcgcg ccttcgccga attcgaaatc cgcgacgatc cgcaggcggc gagcgcggct
     7681 tgggacgaaa tccacgcgga cggactcgcg acagcctatc agagcaaggc ctttgtcggg
     7741 ctttggctgc gcacggtcgg ccgcgccgaa gccgtcgagc ccatgatcgt cctggttcgc
     7801 gacgagcgcg gcgggatcag cgccctgctg ccgctcgcgg tgcgctggcg cctcggcctg
     7861 cgcgtcgccg aatttgtcgg cggcaagcac gccaattttc acatgggcgt gttccgcgcc
     7921 ggtcttgccg tcgatcgcga cgaggtcctc ggcctgttgc ggcgcatcgg cgcatcggcg
     7981 cgactcgacg ccttcttttt cgtcaatcag ccggaactgt ggcaaggctc gtccaatccc
     8041 ctccgcgccc tcggcggcca gaagagtccc agcttcggtc acgccacgca gcttcaaaag
     8101 aatttcgacc aatggttcga agcgcactat tccaaggccg cgcggcggaa attgcgtaag
     8161 aagacgcgcc gtctcgccga gcgcggcgcc gtcgcctcct tcgtcgcgca agacgaggcc
     8221 tcggcgcgcg aggcgctcgc ggcgctcttc gcgcataaag cggcgcgcgc caaagcgaca
     8281 ggccaggtca atgatttcga cgatccggcc gcggtgcgct ttctggagat tgcggcatgc
     8341 gaacgcgccg ccgaacacgg cgccttgatc gagctgcgcg ccatgcgctg cggcgagcgg
     8401 atcgtcgccg tgtttggcgg gctcgctcat gccggccgct tttacggcgt ggtcacctcg
     8461 cacgatccgg acgccgagat cgcgcgatca agcccaggcg aactgctgat cctcgacgtc
     8521 atccgcgacc ttcatgagcg cggcttcaag accttcgatc tcggcgtcgg cgaggcgcgc
     8581 tacaaggacg ccgtttgcga aacgagcgaa gcgctgttcg attccgcgct cggcctgacc
     8641 ttcaagggac gcgccgcgag cctttgcttt ctgctcgcgc gacgcgtcaa gcgctggatc
     8701 aagcaaaggc cttgggcctg gtcgctggtc gcgccgatgc ggcgacggct gcggccgagc
     8761 taacagcgcg atctacgccg ctctttcgag acgcgagcgc cgcccggcgg cgccgcagcc
     8821 gatcacgacg caagcggcgc cgatttgccc gcgcgcttca gcgatcgcgc tcggcaagac
     8881 tgtttgcggc gcgacgatga tcatttcgcg gatcgcttcg gcggcgacct gcgccgcctc
     8941 gtctcgccaa tcgccggcat gggcgacgac gaaatcgtaa gtctcggcta aggcgaccag
     9001 cgcctcgtcg agcgcatcgc cgaccgaggc ggaagcatcg ccggcctgca taatgtctag
     9061 acgcgacgac gcgtcgcggc gcagcgccgc cgcgaagctc gcttcgccgg ccagcacctc
     9121 gctgagcccg acaaggcgat caccctcggg cggcgaaatt tcgctcagcc agttttgtgg
     9181 tcccgggccg agatcgacca gcacggcgcg gcctttcgcg gccagcgcgc ggccaagctc
     9241 gagcgccatt ccccgactgg cgccgtcgcc gcagccggcg atcagcgcaa agcgcggctc
     9301 gcctgtccgc ccctgacggt cgacatgggc gatcagttcg ccgagaccgg cgatctggcc
     9361 ggccgagccg gacgcgtcgt cttgatcgac gctttccgaa gcgcgcgccg gcaagggcgg
     9421 cggcgggacg cccgcagcct gctcgtcggg ccggcgaatc ctctccggct cgatccgcgc
     9481 cgcagcgaca ggccgccgca cgaccggggc ttcttcgccg tcttcgatca gggcgcgcga
     9541 gacgacgatt ccggccgaga gcagaaagcc ggccaaagcg ccaagcagca gggtcgggat
     9601 tttcttcgga aatgtcggat agcgcggctc ggtcgccggc gcgatgacgc gggcgtcggc
     9661 cggcgccgca ttgtcggcgt ccctggcgat cgcctcgcga tatttctgta cgtacgattc
     9721 aagctggtcg cgcgccgcgt tggcgtcgag ctggagcgcg cgcagttgca cgtcctcctg
     9781 attgcccgcg gcgacggttt tggcctgggc ggcgagcgtc tcctgcaaag ccttgaccct
     9841 atcgccggcg ccgcgcgctt cgttctccaa agcccgcgcc gctttctccg cggcgaggcg
     9901 gatttgcgaa tcgatctcgg ccaattgcgc tgaaagcgcc ttcatgcgcg gatgctccgg
     9961 caggagcgtg cgcgattctt gcgcgatttg cgccttgagc gtcgcgcgct gctccgtgag
    10021 ccggcgcaag gcgtcgtcgc gagcgacgtc gggatcttca tcgagccggc cattccgggc
    10081 gaggctgcgc agcatctgcg ccttggcctg cgacgtcgcc tgcgcgctgc gcgcggcggc
    10141 gagttgcgcg ttgatgtcgg caagttgctg gcttggcgcg gtcaggctgt tgccggtcga
    10201 cagcaggccc gactgggcgc gaaacgcctc gaccttgccg tcagcgtcgg cgaccttggc
    10261 gcgcaattcg gtgatcttgg cggcgagcca ggcgctcgcg gctttcgccg cgtccttctt
    10321 cgcctcctcc tgctgatcga ggaagagctg ggcgacgaca ttggcgccgc gggcggcgaa
    10381 ttcggcgtcc tgcgagacga actcgatctg cagcacgcgc gttttcgcga ccggaaaagc
    10441 gttcagatga gacaggaacg tatcgaccag ccgatcttcc gcggttcgtc ccggcggcgg
    10501 ctgacgcgtc agaccgatca gcgacaatag gctttccgct acgcccggcg ccgcggctcg
    10561 actgaattcc gggcgattgg cgaggtcgag cttgacgatc gccttgcgcg ccagggcggt
    10621 cgacatgatc gcctcggcct ggctctgcac ggcttccgga tcgggcgccg tcgtcgcctc
    10681 ctgcggcgct ttgtccggcc gcgtgaaata gctctcctga ttgaccagga gcactttggc
    10741 gacgccggta tagcgcggat gaacgagggc gatgaccgcc agcgatgcgg cgaaggcgag
    10801 cgcggtcggg acgatgatcc agctcttgcg ccgcctgacc gatcgccaga gcttcggcag
    10861 atcgatcgcg tcgccgccgg cctccgctct ttccgtccgt tggttactca tcaatatgct
    10921 ccgccggcgc gcacgccgcg cctcgtttat ggtcgattaa ggttgccgcg aggttaagcc
    10981 gtggcggccg gagcggacgc ccggcgcggg ctattggctt ttcgttaacc atgcctcgat
    11041 agacaggaga ggtctgaaag aaccgggcgc ggctcaacgc tcatgcgcag cttccatttc
    11101 gctttcatcc tcatcgtgct cggcgcgctg accggctgcg ccgcccggcc ctacaacgcg
    11161 gagttctttc gcgctcagat caacgcgccc tatcagctcg ccagcggcga ccgcgttcgc
    11221 gtcatcgttt tcggccagga ttcgctgagc aattccttct cggtcgaccc ggccggcaat
    11281 atttcgatgc cgctgatcgg cttggttaaa gcctatgggc tgacgaccgc cgagcttgag
    11341 cgcgagatcg cggcgcgcct acgcaacggc tacattcgcg atccgcgcgt ctcggtcgaa
    11401 gtcgaggcgt ttcggccgtt ttttgtgctc ggcgaggtcc agaccgccgg gcaatatccc
    11461 tatgtcagcg gcttgacgat ccagagcgcg gtggcgatcg ccggcggctt ttcgccgcgc
    11521 ggctaccagg ccggcgccga cgtgacccgc atggtcaatg gacgcctgat caccgcctgg
    11581 gagccgctga ctttccctgt caggcccggc gatacgctca caattcggga acgtttcttt
    11641 taggttatcg ccctattgga gggactccgg aaaagtccag gagttccggc gatgagcgcc
    11701 gcccgcagct tcgacgccca aaaactgcgc atcgtccacg tctttcgcgc gccgctcggc
    11761 ggcttgtttc gccatgtgat cgatctcgcc tgcgagcaag ccgcccgcgg ccacgatgtc
    11821 ggcctgttct tcgacagcga atttcgcggc gacaatgtcg ccgctgcgct cgcccgcctc
    11881 caaggcggcc ttcgcctcgg cgcgcatggc tgcccgatca agcgcaatcc gcatcccggc
    11941 gatattgggg cgatgctgaa attctccgcg ctgctgcgcc ggctgcggcc cgacatcgtc
    12001 catgggcacg gctcgaaagg cggcctctat gcgcgcctgc cgggattttt cgcccgcccc
    12061 gacgggccgg cgcgcgccta tacgccgcat ggcgggagtt tcaacttcaa gcccggaacc
    12121 gcgctgcacc ggctcttcat gacgacggaa aaagccttga gcttcgccac cgacgtgttc
    12181 ctgttcgaga gcgcctatat cgcgcggcgc tacgattcct atgttggcgt cgatcacgga
    12241 atccggcgag tcgtcgccaa cggcgtcggg ccggcggaat tcagtccgat cgaaccggcg
    12301 cctgacgccg ccgatcttct ctacgtcggc gaattgcgcg ccgccaaagg catcgacacg
    12361 ctgctcgagg cgatcgcgct tgccgaagcg cgcttcggca aatcgtggcg cgccgtactg
    12421 gtcggctcgg ggccggacaa ggagatgctg ctcgggcagg cggaaaagct tggcgtcgcc
    12481 cgacaaattt ccttcgttgg cccgctgccg gcgcgccagg ccttccgcct cggccgcatc
    12541 ctggtcgccc cgtcccgcgc cgaatcgttg ccctacatca ttctcgaagc ggccggcgcg
    12601 caattgccga tcgtcgccac caatgtcggc ggcataccgg aaattttcgg cccctatgcg
    12661 gaccggctcg gcccttgcga cgatccagcc gacctcgccg cgcgcatcct tgcggcgatg
    12721 gacctttccg aggccgaccg aaaggcgcga tgcgccgagc ttgccgctca cgtcgcggcc
    12781 aatttctcga tcaagaccat ggtcgattcc gtgctggaag gctatcgcgc cgcgctggcg
    12841 cggcgtctgc cgcgccaagc cggcgaagcc gtcgcgaaag tcgcccccac cgcgtgaccg
    12901 ccatgaacgc cttttccccc aagaaactgc gcgctgtccc aaatcccgca tcgccgcgtc
    12961 cgccgcaggc cgccgcgtcg cgcccgaaac ggctcgaaga actcgccgcc gagccgacca
    13021 agccgcgcta ctcttccgtc gtgctcgccg gcctggtgcg cgcggcggaa ttcatcctga
    13081 tcgtaatctc cgcgaccctg gtccatcggc tctacgtcgc gccgagcgtc ggctatgcgc
    13141 cgagctattt catcatcgcg gccgccgtcg cgttcctttc gatggtcgtt ttccaaggct
    13201 tgcagaccta tcacccgacc gcgttccgtc agccgatccg gcaggtcttc cgaatcgcgg
    13261 gcggctggtc attcgtcttc ctgatcgtct tcgcagcgat cttcttcctg aagctggacg
    13321 gcgtcatctc ccgggtctgg gtcgcgacct ggttcctgct cggcctggcg gcgctcgccg
    13381 tcgagcgggc gctcctttcg cgcatcgccc tggccatggt ccgcgccggc cgacttgacc
    13441 ggcggacggt ggtggtcggc ggcggcgaat tcggccgcga cttgctgcgc gaccttgcca
    13501 aggcgcgcca ttccgaaatc aagatgctcg gcgtcttcga cgatcgcggc gacgatcgcg
    13561 cgcccgaaac cgtcgaaggc ttcgccaagc tcggcaatgt cgacgacctc gtcgaattcg
    13621 cccgccacac tcggatcgat ctcgtcatct tcgctctgcc gatcaccgcc gagcagcgca
    13681 tcctggaaat gctgcgcaag ctctgggttc tgccgatcga cataaggctc gccgcccacg
    13741 ccaaccgtct gcgcttccgg ccgcgctctt attcctatgt cggccatgtc ccggtcctcg
    13801 acattctcga caagccgatc gccgactggg acgtggtgat caagctcgcc ttcgacaaga
    13861 tcgtgggggg actggcgctg atcgcgctct cgccgatcct tttggcgacg gcgctcgcgg
    13921 tcaagctcga ctcgccgggc ccggtcctgt tccgccagaa gcgctacggc ttcaacaacg
    13981 agatgatcga agtcttcaag ttccgctcga tgtatgtcga tcagaccgac aacaacgcgg
    14041 ccaagctcgt cacgaaagac gatccgcgcg tcacccgcgt cggccgcttc atccgcaaga
    14101 cctcgcttga cgaattgccg cagctcttca atgtcgtgtt caaaggcgat ttgtcgctgg
    14161 tcgggccgcg gccgcacgcg gttcacgcca aagccgccga tcgccaatat gatgaagtcg
    14221 tcgacggcta tttcgcccgc catcgcgtca agccgggcat taccggctgg gcgcagatca
    14281 acggctggcg cggcgagacc gacacgcccg agaaaatcca gcagcgcgtc gagcacgatc
    14341 tctactacat cgagaactgg tcgatcgcgc tcgacctttc gatcctggcg aggaccccgt
    14401 tcgcgctgct gcgcaccaag aacgcctatt gagatttttc gatctgccgg agcaaaaacc
    14461 ggccggcgct
//
LOCUS       DATGJW010000081         2614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_844176, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000081 DATGJW010000000
VERSION     DATGJW010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2614
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_844176"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..917
                     /locus_tag="VKU03_02470"
     CDS             <1..917
                     /locus_tag="VKU03_02470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645757.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLJ70153.1"
                     /translation="WNAGAAIVALNKRFEPRATLDDLVAHKVTSFCAPPTVWRHLIQF
                     DLAQWRTVLREVNSAGEPLNPEVIDQVRRAWGVVIRDSYGQTETTMMVGNSPGQKIKP
                     GSMGRPLPGYRIALADADGSLGDQGEICIALDPRPVGLMRGYQDEAGAIQPLDGPLYH
                     TGDVASRDADGYIAYVGRADDVFKSSDYRLSPFELESALVEHEAVAEAAVVPAPDPIR
                     YTIAKAYITVAPGHAADRATALAIFRHVRERLAPFKRVRRIEFAELPKTISGKIRRVE
                     LRGRENELAAKGQRAEGEFRIEDFPELG"
     gene            complement(1191..2153)
                     /locus_tag="VKU03_02475"
     CDS             complement(1191..2153)
                     /locus_tag="VKU03_02475"
                     /inference="COORDINATES: protein motif:HMM:TIGR03476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase
                     domain-containing protein"
                     /protein_id="HLJ70154.1"
                     /translation="MKPFSIAALIVGLAISVAVIVSFGVKEIATAFLRLGAPGFILLC
                     IAHAPVVAVVGLAWRFCLPKWSAVRVPGVIAARMLREAGAEVLPLSELGGYVIGARAA
                     VLAGATSLEAAASTLLDLTTEAVAQLGFVALGLAALYQLRPDAPLLRPTAVAVAILIA
                     LVAFAALFAERSVKFVAPARAAIRRWFVLFGDTEKAFAVMAAISRRRGAIAAALALHL
                     LGWLGVAAEAWLALRLLGAPIPYAAALVLESLLYAARSLGFFAPNAVGVQEGAYALLA
                     PLVGLNVTEALALSLVKRARDIVIGAPTLLLWQRAEAARLLRRR"
     gene            complement(2167..>2614)
                     /locus_tag="VKU03_02480"
     CDS             complement(2167..>2614)
                     /locus_tag="VKU03_02480"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLJ70155.1"
                     /translation="PLGEILAAEAYRLNGHLAENPSLRMDSAVRRRTLAGREIRAHRL
                     GAILDHREMVKRAFALIFERFDAVLTPTTPFPAPLLSEYDENAPPSLFTRFVNYLDLA
                     ALSLPMGATATGLPVGMQITVPALREPRALEIGAALEADRGALNLA"
BASE COUNT          490 a          850 c          914 g          360 t
ORIGIN      
        1 catggaacgc cggcgcggcg atcgtcgcgc tcaacaagcg cttcgagccg cgcgcgacct
       61 tggacgacct cgtcgcccac aaagtgacga gcttctgcgc gccgccgacc gtctggcgcc
      121 atctcatcca gttcgatctc gcgcaatgga ggacggttct gcgcgaggtc aattccgccg
      181 gcgagccgct caatcccgaa gtcatcgacc aggtgcgccg cgcctggggc gtcgtcatcc
      241 gcgattccta cggccagacc gagacgacga tgatggtcgg caattcgccg ggacaaaaga
      301 tcaagcccgg ctcgatgggc cggccgctgc ccggctaccg catcgcgctc gccgacgcgg
      361 acggctcgct cggcgatcag ggcgagatct gcatcgcgct cgatccgcgc ccggtcgggc
      421 tgatgcgcgg ctatcaggac gaagccggcg cgattcagcc gctcgacggg ccgctctatc
      481 ataccggcga tgtcgccagc cgcgacgccg acgggtacat cgcctatgtc ggccgcgccg
      541 acgacgtgtt caaatcctcc gactaccggt taagcccgtt cgagctggag agcgcgctgg
      601 tcgagcatga ggcggtggcc gaggccgccg tcgtgccggc gcccgatccg atccgctata
      661 ccatcgccaa ggcctatatc acggtggcgc ctggccatgc cgctgaccgg gcgaccgcgc
      721 tggcgatttt caggcatgtg cgcgagcggc tcgcgccgtt caagcgggtg cgccgcatcg
      781 agttcgccga attgccaaag acgatttccg gcaagatacg ccgcgtcgaa ttgcgcggcc
      841 gagagaacga attggctgcg aaggggcagc gcgccgaggg cgagttcaga atcgaggatt
      901 ttccggagct cgggtgaggc gcgcgagccg gagatgaaca ttgtcattct cgtcggcgcg
      961 tcgggggccg gtaagacggc gatcgccagc gcggttgcga cgataaaact cggatggcga
     1021 gattgttcgt cggtcgaaaa cagcctgaac tcgcggacgc cccgatgatg aattgggcga
     1081 catggctccg aaccgatgca atcgagcggg ggtgatgggc gtcttgcgga ggagctgccg
     1141 agggcgcgag gttatagtcg cggtcgtcga tcgcggctac acggcgcgcc tcagcgccgg
     1201 cgcagcaacc gcgcggcttc ggcgcgctgc cagagcagca aggtcggcgc gccgatgacg
     1261 atgtcgcggg cgcgcttgac cagagacaaa gccaacgctt cggtcacgtt cagcccgacc
     1321 agcggcgcga gcagcgcata agcgccttcc tgaacgccga ccgcattggg cgcgaagaaa
     1381 ccgaggctgc gcgccgcata aagcaagctt tcgagaacca aagccgcggc gtaagggatc
     1441 ggcgcgccga gcaggcgaag cgccagccag gcctcggcgg cgacgcccag ccaaccgaga
     1501 agatgcaagg cgagcgccgc ggcgatcgcg ccgcgccggc gcgagatcgc cgccatcact
     1561 gcaaaggctt tctccgtgtc gccgaacagg acgaaccagc gccggatcgc ggcccgcgcc
     1621 ggcgcgacga atttcacgct gcgctccgcg aacaaagcgg cgaaggcgac gagcgcgatc
     1681 aggatcgcaa cggcgacggc ggtcgggcgc agcaaggggg cgtcgggacg caattggtag
     1741 agcgcggcga ggccgagcgc gacgaagccg agctgggcga cggcttcggt ggtcagatcg
     1801 agcaaggtcg aggcggcggc ttccaggctg gtcgcgccag ccagaaccgc ggcgcgcgcg
     1861 ccgatcacat agccgcccaa ttcggagagc ggcagcactt ccgcgccggc ttcgcgcagc
     1921 atccgcgccg cgatcacgcc gggaacgcgc accgccgacc atttcggcag gcagaatcgc
     1981 caagcaagtc cgacgaccgc gacaaccggc gcatgggcga tgcacagcag gatgaagccc
     2041 ggcgcgccaa gccgcaggaa ggccgtggcg atttccttga cgccgaagga cacgatgacg
     2101 gcgacgctga tcgcgaggcc gacgatcaac gctgcgatgg agaaaggctt cactttgcta
     2161 tcggcttcac gcgaggttca gggcgcctcg atccgcctcc agcgccgcgc cgatctccaa
     2221 agccctgggc tcccgcaagg cggggaccgt gatttgcatg ccgaccggca agccggtcgc
     2281 cgtcgcgccc atcggcagcg agagcgcggc aagatcgaga tagttgacga aacgcgtgaa
     2341 cagcgaaggc ggcgcgttct cgtcatactc gctgagcagc ggggccggga acggcgtggt
     2401 cggcgtcagc acggcgtcga atcgctcgaa aatcagtgcg aaggcgcgtt tgaccatttc
     2461 gcgatggtcg agaatcgcgc cgagccgatg ggcgcgaatc tcccggccgg caagagtccg
     2521 ccggcgcacg gcgctgtcca tgcggagcga aggattttcc gccaaatggc cgttgaggcg
     2581 ataggcctcg gcggctaaaa tttcgccgag cggg
//
LOCUS       DATGJW010000082         3925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_222339, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000082 DATGJW010000000
VERSION     DATGJW010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_222339"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1448
                     /locus_tag="VKU03_02485"
     CDS             <1..1448
                     /locus_tag="VKU03_02485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADPH--cytochrome reductase"
                     /protein_id="HLJ70156.1"
                     /translation="AIPRRIDERLAALGAKRVYERGEGDARADLDGDFERWFAALRPV
                     AAKELGIDVDFTSGAGGEPLYQVEKVAAAGGDDYGASVGATLIKILVNDELQNRNGAN
                     GSARSTRHIEVALPEGATYRVGDHLSILPRNDPALVDAVARRFGFNVSDAVRLQTSAG
                     RRAQLPVGEPIAIGRLLGERVELQQTATRRHIQIMADHTRCPVTKPKLLALAGEDAAA
                     TERYRTEIFARRRSIYDLLAEHRACELPFGVFLEMLPPLSPRYYSISSSPSRDAQRCS
                     VTVAVVAGPALSGSGEFKGVCSTHLAGRSAGDTVYASIRQTKAGFQLPADPATPIIMI
                     GPGTGLAPFRGFLQERAAQKAKGAALGSAILFFGCRHPDQDFLYADELKAFAAEGLVE
                     LHVAFSRAQETKTYVQDLIKAQAETVSGLIERGAIIYVCGDGSKMEPAVKRALIEIHR
                     HRAGVDDAAAERWIEELGRQNRYVLDVWAGG"
     gene            1782..2204
                     /locus_tag="VKU03_02490"
     CDS             1782..2204
                     /locus_tag="VKU03_02490"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HLJ70157.1"
                     /translation="MTETENTIVAPAAARDFVKKAAEAAKERANTFHANAEKATANAE
                     QTLVTAVGEVAKLARQAQQAAYEDAEAFFSGVGKLASAESVGEAVQIYADYMRARTDV
                     VTARTRAATDYVGKLFADGAKSVQENVAKASAFAQKAA"
     gene            2365..3135
                     /locus_tag="VKU03_02495"
     CDS             2365..3135
                     /locus_tag="VKU03_02495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ70158.1"
                     /translation="MSIASESLPPPSRALLMLEGRAIHEFGAFLASLPLLRLAPRGDG
                     RPVLVFPGLVASDLSTRPLRTFLSNHGYAVRGWGQGRNFGPRDGVVDGMLGLLRDMAD
                     ESGRKVSLVGWSLGGIYARQLAKMLPDHVETVITLGSPFAAHPRSTNAWRVYEFTSGK
                     SADDRDDAFAHSLADPPPVPTTAIFSRSDGVCAWRGCMERPGALAENIEIQGSHCGLG
                     HHPAVVYAVADRLAQKEGAWRPFDRSGWRALLYPDPTR"
     gene            complement(3140..>3925)
                     /locus_tag="VKU03_02500"
     CDS             complement(3140..>3925)
                     /locus_tag="VKU03_02500"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLJ70159.1"
                     /translation="FCGRLADRGFRVIRFDNRDIGLSTKFESAPKVDIGATMMRAIAG
                     LPIKAAYNLDDMAKDAVGVLDALAIAKAHIVGASMGGMIAQIIAAKYAERARSLVSMM
                     SSSGDPKLPQATPAAAAALTTPRPSQSDREASIQFGMNAYRVIGSPAYPAPEPELRAK
                     IERAFDRSYYPAGVARQMVAILASGSRVDLLPTIKLPTLVLHGGDDPLVPVEAGKDTA
                     RLVPGAKLKIIPGWGHDIPTALIPTLVEAIATHCEAADRAAAA"
BASE COUNT          704 a         1236 c         1268 g          717 t
ORIGIN      
        1 aagcgattcc acgccgtatc gacgagcgtc ttgcggcgtt gggcgccaag cgcgtctacg
       61 aacgcggcga gggcgatgcg cgcgccgatc tcgatggcga tttcgagcgc tggttcgcgg
      121 cgctgcggcc ggtcgcggcg aaagaactag gcatcgacgt cgatttcacc agcggcgccg
      181 gcggcgagcc gctctatcaa gtagagaagg tcgccgctgc cggcggcgac gactacggcg
      241 catcggtcgg cgcgacgctg ataaaaattc tcgttaacga cgagttgcag aatcgaaacg
      301 gcgccaatgg ctccgcgcgc tcgacgcggc atatcgaggt tgcgcttccc gaaggcgcga
      361 cgtaccgcgt cggcgaccat ctcagcatcc tgccgcgcaa cgatccggcg cttgtcgacg
      421 ccgtggcgcg gcgcttcggc ttcaatgttt ctgacgccgt ccgcctgcaa accagcgccg
      481 gacggcgcgc gcaattgccg gtaggcgagc cgatcgccat cggacgtttg ctcggcgagc
      541 gggtcgaact gcagcaaacc gcgacgcgcc ggcacattca gatcatggcc gaccatacgc
      601 gctgcccagt gaccaagccg aaactcctgg ctttggcggg agaggatgcg gcagcgaccg
      661 agcgttatcg tacggagata ttcgcgcggc gcaggtccat ctatgatctg ctcgccgagc
      721 atcgcgcctg cgagcttcct ttcggcgtct tcctggaaat gttgccgccg ctgtcgccgc
      781 gctattactc gatttcatct tcgcccagcc gggacgctca acgttgcagc gtcaccgtgg
      841 cggtcgtcgc cggacccgcc ttgtcaggct cgggcgaatt caaaggcgtt tgctcgacgc
      901 atctggcggg gcgaagcgcc ggcgacacgg tctatgcttc catccggcag accaaggccg
      961 gcttccaatt gccggctgat ccggcgacgc caatcatcat gattggtcca ggtactggcc
     1021 ttgcgccttt ccgcggcttt cttcaggagc gggccgctca gaaggcgaag ggcgccgcgc
     1081 tcgggtcggc gatactgttt ttcggctgcc ggcatcccga ccaggacttt ctctacgccg
     1141 acgaactcaa ggccttcgcc gccgaggggc ttgtcgagct gcacgtcgcc ttctcgcgcg
     1201 cccaggagac gaagacttac gttcaggacc tgatcaaggc gcaagcggag acggtcagcg
     1261 gcctcatcga gcgcggcgcc attatctatg tctgcggcga cggatcgaaa atggagccgg
     1321 ccgtcaaacg ggcgttgatc gagatccatc gccatcgggc cggcgtcgat gacgccgccg
     1381 ccgaacgttg gatcgaggaa ctgggccggc aaaaccgtta tgtgctggac gtttgggccg
     1441 gcgggtagtc agcgaagaat actttgtgct catgagcaac ccggcgcgtt caagaatcgc
     1501 ctgtatgccg agccggcgca cgacgttgag caagccagag ctgcgccgat cggcatttcg
     1561 acgccgcgct cgagctttga gaggtagccc acggtcaggt tcaggtattt ggcgaagacg
     1621 gcccggctca tgggcggcaa acaagagctg ggtgatcacg aaaccgtgat ggtgcattgc
     1681 aatattaact gttgcattgc aatatgtctt atgagagaag aactgatccc tgggccaata
     1741 acggacaggg ctgaccatag ctctcaaaga ggatggaaat catgaccgag acagagaaca
     1801 caatcgtggc gcctgccgcc gcccgcgact tcgtgaaaaa ggccgctgag gctgctaaag
     1861 agcgcgcgaa caccttccac gccaatgccg aaaaggcgac ggccaatgcc gaacagacgc
     1921 ttgtgaccgc ggttggcgaa gtcgccaagc tcgcccgtca ggctcagcag gccgcctatg
     1981 aggacgcaga ggcgttcttc tccggcgtcg gcaagctggc ttccgccgaa agcgtcggcg
     2041 aagcggttca gatctatgcc gactatatgc gcgctcgcac cgacgtcgtc actgcgcgca
     2101 cccgcgccgc taccgactat gtcgggaagc tttttgccga tggcgccaag agcgttcaag
     2161 agaacgtcgc caaggccagc gctttcgctc agaaggccgc gtaaacggct gcctccaagc
     2221 gacttcgaga ggcccgcgtg agcgggcctt tttattttgg acctttcgac acgccgcccg
     2281 tgtttgaaat cggaggcgaa cgcgcctaac tatttgcaga gcagagccgg ccaagcggcc
     2341 cttgaggctc ggaggaacaa aagcatgagc attgcttccg aaagccttcc tccgccctcc
     2401 cgcgcgttgc tgatgctcga ggggcgcgcg attcatgaat tcggcgcgtt ccttgcgagc
     2461 ttgccactgc tgcgtctcgc cccacggggg gacggccgcc cggttttggt cttccccggc
     2521 ttggtcgcaa gcgatttgtc gacgcggccg ctgcggacat tcctttcgaa tcacggctat
     2581 gccgttcgcg gctggggcca gggccgcaat tttggtccgc gcgacggcgt cgtcgatggc
     2641 atgctcgggc tgcttcgcga catggcggac gagagcgggc gcaaggtgag cctggtcggc
     2701 tggagcctcg gcggcatcta tgcgcgccag ctcgccaaaa tgctccccga tcacgtcgag
     2761 acggtcatca cgctcggcag tcctttcgcc gcccatccgc gttcgaccaa cgcttggcgg
     2821 gtctatgaat ttaccagcgg caaaagcgcc gacgatcgcg acgacgcttt cgcgcattcg
     2881 cttgccgatc cgccgcccgt gccgacgacg gcgattttca gccgtagcga cggcgtttgc
     2941 gcctggcgcg gctgtatgga gcggccgggc gcgcttgcgg agaatatcga gattcagggc
     3001 agccattgcg gcctcggcca tcaccccgcc gtcgtctatg cggtcgccga ccgtctcgcc
     3061 cagaaggaag gggcgtggcg gcccttcgat cggagcggct ggcgcgcgtt gctctatccc
     3121 gatcccacgc gatagcgctt taagccgctg cggcgcgatc cgcagcttcg caatgcgtgg
     3181 cgatcgcctc gaccagcgtc gggatcagcg cggtgggaat atcgtgtccc cagcctggaa
     3241 tgatcttgag cttcgcgcct ggcaccagac gcgccgtatc cttgcccgct tcaaccggca
     3301 cgagcggatc gtcgccgcca tgaagcacca gcgtcggcaa tttaatcgtc ggcagcaggt
     3361 cgacccggct gccgctggcg agaatcgcga ccatctgacg ggcgacgccg gccggatagt
     3421 acgatcggtc gaaggcgcgt tcgatcttgg cgcgcaactc gggctcgggg gccggatagg
     3481 ctgggctgcc gatcacccga taggcgttca tgccgaattg gatcgaggcc tcgcgatcgc
     3541 tttgcgaggg gcgcggggtc gtcagcgccg ccgcggcggc gggcgtcgct tgcggcagtt
     3601 tcggatcgcc gctcgacgac atcatcgaca cgaggctacg cgcgcgttcg gcgtatttgg
     3661 cggcgatgat ttgcgcgatc atgccgccca tcgaagcgcc gacaatatgc gccttggcga
     3721 tggcgagcgc atccaagacg ccaacggcgt ccttcgccat atcgtcgaga ttataggcgg
     3781 ccttgatcgg caggccggcg attgcgcgca tcatcgtagc gccaatatcg acttttggcg
     3841 cgctctcgaa tttggtcgaa aggccgatat cgcgattgtc gaaacggatg acgcgaaatc
     3901 cgcgatcggc gagcctgccg caaaa
//
LOCUS       DATGJW010000083         5845 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1155115, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000083 DATGJW010000000
VERSION     DATGJW010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5845)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5845)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5845
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1155115"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(341..1153)
                     /locus_tag="VKU03_02505"
     CDS             complement(341..1153)
                     /locus_tag="VKU03_02505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RMD1 family protein"
                     /protein_id="HLJ70160.1"
                     /translation="MNDTAPRVGTRLTARALIVGDRIDTAGLERPDMIAATPLAFRVG
                     ADGMVALFRFGVAVMVGLSPLEEEDLLAKLKLRAAGPSARGDDETAHLEIAPEFDDRA
                     PPGGPIQMKDLSPERFLVLADALAKTVALGRDERQVNAVFDVIEPFAARLAESGRTPF
                     RRAAMLKLIGQALLAQHRVSGRVAVEEKPDVLWDRPDLERLYARLEDEYELKERAAAL
                     KRKLDVIVETAHALTDIIDADRATRLEATIVVLIVVEVVLGLYQIFALGHAH"
     gene            1402..1602
                     /gene="rpmI"
                     /locus_tag="VKU03_02510"
     CDS             1402..1602
                     /gene="rpmI"
                     /locus_tag="VKU03_02510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L35"
                     /protein_id="HLJ70161.1"
                     /translation="MPKLKTKSGAKKRFKITGTGKVVYAQKGKRHMMIRRTNKQIRNL
                     RGTSILFKTDGEKVKKYFLPNG"
     gene            1639..2004
                     /gene="rplT"
                     /locus_tag="VKU03_02515"
     CDS             1639..2004
                     /gene="rplT"
                     /locus_tag="VKU03_02515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HLJ70162.1"
                     /translation="MSRVKRGVTAHAKHKKTLRAAKGYFGRRKNTIRVAKQAVEKAMQ
                     YAYRDRKNRKREFRALWIQRINAAVRDHGLTYSRFIAALTTAGIEVDRKVLSELAIHE
                     PAAFAAIVEKAKGHLPKAA"
     gene            2131..3213
                     /gene="pheS"
                     /locus_tag="VKU03_02520"
     CDS             2131..3213
                     /gene="pheS"
                     /locus_tag="VKU03_02520"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HLJ70163.1"
                     /translation="MTDLLELETQTLAAIDDAGDEAALEAVRVGALGKKGVISALLAS
                     LGKMAPEERKVQGAAINSLKDKVSEALVSRKAALKARAREAQLAAEAVDVTLPARTSG
                     LETGRIHPITQVTDELTAIFADMGFSIAEGPDIESDDYNFTKLNFPPGHPARDMHDTF
                     FFPPDETGERKLLRTHTSPVQVRTMLAQKPPIRVICPGRTYRCDADQTHTPMFHQVEG
                     LVIDRTANLGQLKWVLEEFCKAFFEVPRVAMRFRPSFFPFTSPSMEVDIQCRRSGGEI
                     RFGEGEDWLEILGCGMVHANVLRNCGLDPDVWQGYAWGMGIDRIAMLKYGMPDLRDFF
                     EADIRWLDHYGFRPLDLPTLAGGLSG"
     gene            3359..5770
                     /gene="pheT"
                     /locus_tag="VKU03_02525"
     CDS             3359..5770
                     /gene="pheT"
                     /locus_tag="VKU03_02525"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HLJ70164.1"
                     /translation="MKFTLSWLKDHIETSASLDEIVETLTRIGLEVENVENPAAKYAG
                     FVVARVIEAKQHPNADRLRVCLVDAGGEAVQVVCGAPNARTGMKSVFSPVGTYIPGKK
                     ITLAKGTIRGVESNGMLCSAAELELSNDHEGIIDLPADAPVGAAYAAYAGLDDPVIEI
                     NLTPNRPDATGVSGIARDLAAAGLGALKTKAPAAIAGTFDCPVGVRLDFAPGDAHLCP
                     AFALRLVRGVRNGASPEWMQKRLRAIGLRPINALVDITNYVTFDRGRPLHVFDAAKVA
                     GDLSVRRARKGEEVLALDGRTYALDDGIVVIADEKGIESIAGIMGGEHSGCDEKTTDV
                     LIESALWDPANIAQSGRKLGISTDARYRFERGVDPAFCVPGAELATRMVVELCGGEPS
                     RLVVAGAPPKGTKIVDFPYSEVRRLTGLDIPKVEGEAILAKLGFKVLDDSKVEAPSWR
                     PDIDGKADIVEQIVRIAGLDQVPPNPLERAEPSVAKPVLTLLQKRTRTAKRALAALGL
                     REAVTWSFIAKPTAELFGGGDPALALANPIAADLSDMRPSLIPGLAAAAERNARRGFR
                     DIGLFEVGQIFLGTGERDQRIAAAALRRGAAKAEGAGRHWLGGGGAVDPFDAKSDVFA
                     LLAALGVPTGGLQIVPGGPRFLHPGRSARLQFGPQNAIGWFGQLHPNALEALDVEGPV
                     AAFEIILDALPAPKARPTKAKAKLERSEFMPVERDLAFVVEKRVPAADLVRAAQAAER
                     NLIASAQVFDVYEGQGVPAGRKSVAIAITLQPKDRTLTDAEIDAVIAKIVAEAAKKTG
                     AALRG"
BASE COUNT         1043 a         1939 c         1903 g          960 t
ORIGIN      
        1 ggcctcgata aggtcgcggt tgaggacgcg gcggccctcg cgcaagcgtt cgattgggcc
       61 gagcagggga tggacttcgc cgatgcgctt catctgacga aagcctcgca ttgtgaagct
      121 ttcataagct tcgaccgcgc cctcgcgaaa gcagcgcgca aagcaaaggc tctcgaagcg
      181 cgtgagcctg atatgcgttg aattgtagcc gcgtcggcga ccgcggctct atcgcggcgc
      241 ctgatgaaag ccgcggcgcg ctttccctgc cgctggagga tgtcgccacg gggcgtcggc
      301 cgcggcgcca gcgcagcgcg gctgaagacc tacgccgcgc tcaatgggcg tggccaagcg
      361 cgaaaatctg gtagaggccg agcacgacct cgacgacgat cagcacgacg atggtcgcct
      421 ccagcctggt cgcgcggtcg gcgtcgataa tgtcggtcag cgcatgcgcg gtctcgacga
      481 tcacgtcgag cttgcgcttc aaggccgccg cgcgctcctt gagttcgtat tcgtcctcca
      541 ggcgggcgta gaggcgctcg agatcggggc ggtcccacag cacgtccggc ttctcctcca
      601 ccgccacgcg gccggagaca cggtgctggg cgagcagcgc ttgcccgatc agcttgagca
      661 tcgccgcccg gcggaacggc gtgcggccgc tctcagccag cctggcggcg aaaggctcga
      721 tcacgtcgaa caccgcattg acctgccgct cgtcgcggcc gagcgccacc gtcttagcca
      781 aggcgtcggc gaggaccagg aaccgctccg gcgacaggtc cttcatctgg atcggaccgc
      841 ctggcggcgc gcggtcgtcg aattccggcg cgatttcgag atgggcggtt tcgtcgtcgc
      901 cgcgcgcgct cggcccggcg gcgcgcagct tgagcttggc gagaaggtcc tcctcttcca
      961 gcggcgaaag accgaccatc accgcgacgc cgaagcggaa cagcgcgacc atgccatccg
     1021 ccccgacccg gaaagcgagc ggcgtcgccg cgatcatgtc cggccgctcc aagccggccg
     1081 tgtcgatccg gtcgccgacg atcagggcgc gggcggttag gcgagtccca acgcgggggg
     1141 cggtgtcgtt catggactat tccttagccg agccccaagg cgccggcggt tgcctgatta
     1201 tgcaacagaa gccggtagaa attggatgcc gccgaatttc cgcatccagc ttgcgcggcg
     1261 gggcgcttgc ttttatagac gcggcttcga accgcccggc tggtaagggc tgccgtggcg
     1321 gttcttttcg ctcattccga cgcgaacggg cctttttaag gcccgctgac caattgaggg
     1381 cgccccctca aggagagcca aatgcccaag ctgaagacca aatcgggcgc gaaaaagcgc
     1441 ttcaagatca ccggaaccgg caaggtcgtc tacgcccaga agggcaagcg ccacatgatg
     1501 atccggcgga ccaacaagca gatccgcaat ctccgcggca cctcgatcct gttcaagacc
     1561 gacggcgaga aggtcaagaa atacttcctg cccaacggct gacctagccg ctcgctcaaa
     1621 cattccgagg agatcgtcat gtctcgcgtc aaacggggcg tcaccgccca cgccaagcac
     1681 aagaaaaccc tccgcgcggc caaaggctat ttcggccgcc gcaagaacac gatcagggtc
     1741 gccaagcagg cggtcgaaaa ggcgatgcaa tacgcctatc gcgaccgcaa gaaccgcaag
     1801 cgcgaattcc gcgcgctttg gatccaacgc atcaacgccg cggtgcgcga tcatggcctg
     1861 acctatagcc gcttcattgc cgcgctgacc acggccggca tcgaagtcga ccgcaaagtc
     1921 ttgtcggaac tcgcgattca cgagccggcg gcgtttgcgg cgatcgtcga aaaggccaag
     1981 ggccatctgc ccaaggcggc ttgacgagag gcgaaaacgc gccttgaacg aaaagcccgc
     2041 cttccggcgg gctttttcgt ctcaaccggc gcgaactcga caagcctttg acggatgtga
     2101 gaatagtcgc gcgcaacgtt ccgaacgcac atgaccgacc tgctcgaact cgaaacgcaa
     2161 acgctcgccg ccatcgacga cgccggcgac gaggccgcgc tcgaagcggt gcgggttggc
     2221 gcgctgggca agaaaggcgt gatctcggcg ctgctggcga gcctcggcaa gatggcgcct
     2281 gaggagcgca aggttcaggg cgcggcgatc aacagcctga aggataaggt cagcgaggcc
     2341 cttgtgtcgc gcaaggcggc gctgaaagcg agggcgcgcg aggcgcaact cgccgcggag
     2401 gcggtcgatg tgaccctgcc cgcgcggacc agcgggctgg agacgggccg catccatccg
     2461 atcacccaag tcaccgacga actcaccgcc atcttcgccg atatgggctt ctcgatcgcc
     2521 gagggcccgg atatcgaaag cgacgattac aatttcacca agctgaattt cccgcccggc
     2581 cacccggcgc gcgacatgca cgacacgttc ttcttcccgc ccgatgaaac aggcgagcgc
     2641 aagcttttgc gtacccatac gagcccggtg caagtgcgca ccatgctggc gcagaagccg
     2701 ccgatccgcg tgatctgccc gggccgcacc tatcgctgcg acgccgacca gacgcatacg
     2761 ccgatgttcc accaggtcga gggcctggtg atcgacagga ccgccaatct cggccagctc
     2821 aaatgggtgc tggaggagtt ctgcaaggcg tttttcgaag tgccgcgggt cgcgatgcgt
     2881 ttccggccga gcttctttcc cttcacctcg ccgtcgatgg aggtcgacat ccaatgccgg
     2941 cgctcgggcg gcgaaatccg cttcggcgaa ggcgaggact ggctggagat tctcggctgc
     3001 ggcatggtcc acgccaatgt cctgcgcaat tgcggcctcg atcccgacgt ctggcagggc
     3061 tacgcctggg gcatggggat cgaccgcatc gccatgctga aatacggcat gccggatctg
     3121 cgcgacttct tcgaagccga catacgctgg ctcgatcatt acggcttccg gccgctcgat
     3181 ctgccgacgc tggcgggagg attgagcggg tgaatttggc gggcgctatt cacaccggcc
     3241 gtcctgcccg gccttgtgca ggacgtccgc gtcgttgtgc gcttcatcgc agcgtcgatg
     3301 gccgggacag gcccggccat gacggctttg gcttttttcg tacttcccga acagcaccat
     3361 gaaattcacg ctctcctggc tcaaggatca tatcgagacc tccgcctcgc tcgacgagat
     3421 cgtcgagacg ctgacgcgga tcggcctcga agtcgagaac gtcgagaacc cggcggcgaa
     3481 atacgccggt ttcgtcgtcg cgcgggtgat cgaggccaaa cagcatccga acgccgaccg
     3541 attgcgcgtc tgcttggtcg acgccggcgg cgaggcggtc caggtcgtct gtggcgcgcc
     3601 gaacgcccgg acgggcatga aaagcgtgtt ctcgccggtc ggaacctata ttcccggcaa
     3661 gaagatcacg ctcgccaaag gtacgatccg cggcgtcgag tcgaacggca tgctctgctc
     3721 ggccgccgag ctggagcttt ccaacgatca cgaggggatt atcgatcttc cggccgacgc
     3781 gccggtcggc gccgcttacg cggcctatgc cggtcttgac gatccggtga tcgagatcaa
     3841 tctcacgccc aaccggcccg acgcgaccgg cgtctcggga atcgcccgcg atcttgccgc
     3901 ggccggcctt ggagcgctca agacgaaagc gccggcggcg atcgccggga ccttcgactg
     3961 cccggtcggc gtgcgcctcg atttcgcgcc gggcgacgca catctttgcc cggctttcgc
     4021 gctgcgcctg gtgcgcggcg tcagaaacgg cgcgtcgccg gaatggatgc agaagcgcct
     4081 gcgcgcgatc ggcctcaggc cgatcaacgc gctggtcgac atcaccaact acgtcacgtt
     4141 cgatcgcggc cggccgcttc acgtcttcga cgccgccaag gttgcgggcg atttgagcgt
     4201 gcgccgggcg cgcaagggcg aagaagtcct ggcgctcgac ggccggacct atgcgctcga
     4261 cgacggcatc gtcgtcatcg ccgatgagaa ggggatcgaa tcgatcgccg gcattatggg
     4321 cggcgagcat tcgggctgcg acgagaagac gacggacgtg ctgatcgaaa gcgccctctg
     4381 ggatcccgcc aacatcgccc agagcggccg caagctcggc atttcgaccg atgcgcgcta
     4441 ccgcttcgag cgcggcgtcg accccgcttt ctgcgttccc ggcgccgagc ttgcgacgcg
     4501 catggtggtg gagctttgcg gcggcgagcc gtctcggctc gtcgtcgccg gcgcgccgcc
     4561 caagggtacg aaaatcgtcg acttcccgta cagcgaagtt cgccgcctca ccggtcttga
     4621 tatcccaaag gtcgagggcg aggcgattct cgccaagctt ggcttcaagg ttctggacga
     4681 ttcgaaagtc gaggcgccgt cctggcggcc cgatatcgac ggcaaggccg acattgtcga
     4741 gcagatcgtg cgcatagccg ggctcgacca ggtgccgccg aacccgctcg aacgcgccga
     4801 gccgagcgtc gccaagccag tcctcacctt gctgcaaaaa cgcacccgaa cggccaagcg
     4861 cgcgctcgcc gctttgggtc tgcgcgaagc ggtgacctgg tccttcatcg ccaagccgac
     4921 ggccgaattg ttcggcggcg gcgatccggc ccttgcgctc gccaatccga tcgccgccga
     4981 tctttcggat atgaggccga gcctcattcc aggcctcgct gcggcggccg agcgcaatgc
     5041 ccgacgcggc tttcgcgaca tcggcctgtt cgaagtcggg cagattttct tagggaccgg
     5101 cgagcgcgac caacgcatcg cggcggcggc cttgcggcgc ggcgcggcca aggccgaggg
     5161 cgcagggcga cattggctcg gcggcggcgg cgcagtcgat ccgttcgacg ccaagagcga
     5221 cgttttcgcg ctgctcgccg cgctcggcgt tccgaccggc ggcttacaaa tagtccccgg
     5281 cggcccgcgc tttcttcatc ccggccgctc ggcgaggctg caattcgggc cgcaaaacgc
     5341 gatcggctgg ttcggccaat tgcatcccaa tgcgctcgaa gcgctcgacg tcgaggggcc
     5401 ggtcgcggcg ttcgaaatca ttctcgacgc tttgccggcg ccgaaagccc ggccgaccaa
     5461 agccaaggcg aagctcgagc gctcggagtt catgccggtg gagcgcgacc tcgcctttgt
     5521 cgtcgagaaa cgggtcccgg ccgcggatct ggtcagggcc gcgcaagcag ccgagcgcaa
     5581 tcttatcgcc tcggcgcaag tcttcgatgt ctatgagggt cagggcgtgc cggccggccg
     5641 taaatccgtc gcgattgcga ttaccttgca acccaaggac cggacgctga ccgacgcgga
     5701 gatcgacgcg gtcatcgcca aaatcgtcgc cgaggcggcg aagaagaccg gcgccgcctt
     5761 gcgcggctga gccgcagacg aggagtcgac cgttcggatg atgatgcgcc gcctcgccgc
     5821 cgtcgctctg ttcgtggcgc tttcc
//
LOCUS       DATGJW010000084         5183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1467192, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000084 DATGJW010000000
VERSION     DATGJW010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5183
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1467192"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..286)
                     /locus_tag="VKU03_02530"
     CDS             complement(<1..286)
                     /locus_tag="VKU03_02530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70165.1"
                     /translation="MDRKAKSDSDTAAARQIDMVAVLLIAFAILTFFYLIHVVLLPFV
                     IAAAIAYILTPLVDFISAKTRAPRAAAALGVFVILLALAGTAVVLGGPALL"
     gene            complement(279..1436)
                     /locus_tag="VKU03_02535"
     CDS             complement(279..1436)
                     /locus_tag="VKU03_02535"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HLJ70166.1"
                     /translation="MELLASSTLLLVVFLLLLRAVRQSRALQVLEPKRAVFGDDAPKV
                     AVILPARNEAANIRACLAGLAAQTYPPARLRILAVDDNSNDGTREIIARFADRRANIT
                     PLRSGPLAKGWTGKSQACWTGAQAACADAEWLCFVDADTCADPRLIASAVAAARTSGA
                     SLLSLAPRQLLLSFAERLMVPCGLYLLGFCQNLSRLQAPDTEAVSATGQFMLVRRAAY
                     EEVGGHAAVSGAICEDLELARLLKRNRHSVVLMDGAKAISVRMYDGWRALWPGFAKNL
                     LDMFGGAAASLSIAAAAVVLSWSLLLAPIADYAHCLGGAPGACVALGLALPAALAALG
                     FHIAGALYLGVPAWYGLLFPLAYSIGAIMVVDAVRLRATGRVPWKGRVYHG"
     gene            2413..4509
                     /locus_tag="VKU03_02540"
     CDS             2413..4509
                     /locus_tag="VKU03_02540"
                     /inference="COORDINATES: protein motif:HMM:NF014026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 3 C-terminal
                     domain-containing protein"
                     /protein_id="HLJ70167.1"
                     /translation="MALNEFSQEEKLSLTHGGLGAPWGDEPKPAGAVGSAGYVAGIPR
                     LGVPALQETDAELGVANPGNIRPGDTATAMPSDLALASTWDEALARRQGETVGAEARA
                     RGFSVLLGGAVNLIRDPRGGRNFEYFSEDPLLSGTMAGAAVAGAQSRHVISTVKHFAL
                     NDQETDRVVLDAEIDRAAARESDLFAFEIALERSRAGSVMCAYNQVDGAYSCENDWLL
                     SQVLKGDWRYPGFVMSDWGAVHSTVASALAGLDQESGEQLDTQNFFDRPLAKAVEDGA
                     VPQARLDDMARRILTSIFASGLYDDPPRRGPIDAAAGDRTALEIAREGLVLLKNDGVL
                     PLAGSVRRLAVIGGHADKGVLSGGGSSQVVPRGGVALAEPSTRVEGDLIFDPSSPLEA
                     LRRQFPQAQIVYDDGRTPAEAAKLAATADAAIVFAYQWMTETADAPDLALPRAQDRLI
                     AAVAAANPRMIVVLETGGPVLMPWLGRAAAVLEAWYPGQKGGEAIAEALSGAIDPSGR
                     SPVTFPRSEAQLPRPTVPGDPNAAPRGPVGRGGHYGRIFTAAYNEGAAVGYKWFAQCR
                     EAPLFPFGFGLSYTRFALSDLTASADGDAVTISAQVQNIGARAGAAIPQFYLSGPSGA
                     AIPLRLAGWSRIDLRPGEARRATIAVDPRLFAHFDESGRRWRIAPGVYRIMAGFDAAH
                     REQATEIGLKGADLPP"
     gene            complement(4587..4751)
                     /locus_tag="VKU03_02545"
     CDS             complement(4587..4751)
                     /locus_tag="VKU03_02545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HLJ70168.1"
                     /translation="MRLYIKKFLSNEDGATAIEYGLIASLIAVVIIGAVSVVGTKLTA
                     TFNEVASNLN"
     gene            complement(4907..>5183)
                     /locus_tag="VKU03_02550"
     CDS             complement(4907..>5183)
                     /locus_tag="VKU03_02550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ70169.1"
                     /translation="LVATKKGMLAALALAAHKPPDLYAYSFARLTKSLGFLLNRAVAT
                     GEMRADVTPEDLFRTLIGLCYSYDQPGWQATVTRLTDVFVDGLVIKQ"
BASE COUNT          884 a         1653 c         1780 g          866 t
ORIGIN      
        1 ggagcagggc gggaccgcca agcacgacgg cggttccggc aagcgccagc aagatgacga
       61 agacgccgag cgccgccgcg gcgcgcggcg cgcgggtctt ggccgagata aaatcgacca
      121 gcggggtgag gatataggcg atcgccgccg cgatgacgaa cggcagcagc acgacatgga
      181 tgagataaaa aaaagtgagg atggcgaagg cgatcagcag aacggcgacc atgtcgatct
      241 ggcgcgccgc ggcggtgtcg ctgtccgatt tcgccttcct atccatgata gacccggccc
      301 ttccacggca cgcggccggt cgcgcggagc cggacggcgt cgacgaccat tatagcgccg
      361 atgctatagg cgagagggaa gagcaggccg taccaagccg ggacgccgag atagagcgcg
      421 ccggcgatat gaaagcccaa ggccgccagc gccgcgggta gggcaaggcc gagcgcaacg
      481 caagcgccgg gcgcgccgcc gagacaatgc gcataatcgg cgatcggcgc cagaaggagg
      541 ctccagctca ggacgacggc cgccgccgcg atggagagcg acgccgccgc gccgccgaac
      601 atatcgagga ggtttttcgc aaatccgggc caaagggcgc gccaaccgtc atacatgcgg
      661 acggaaatcg cctttgcgcc gtccatcagc acgaccgaat gcctgttccg cttcaaaagc
      721 cgggcgagtt cgaggtcttc gcagatcgcg cctgagaccg cggcgtggcc gccgacctcc
      781 tcgtaagccg cgcgccgcac cagcatgaac tggccggtcg cgctcaccgc ctcggtgtcc
      841 ggcgcctgaa gccgcgacag attctggcag aagccgagaa ggtagagacc gcaggggacc
      901 atcagccgct cggcgaagct gagcaggagt tggcggggcg cgagcgagag aaggctggcg
      961 cccgatgtgc gggcggccgc gacggccgag gcgatcaagc gcgggtcggc gcaggtgtcg
     1021 gcgtcgacga agcaaagcca ttcggcgtcg gcgcacgccg cctgcgcgcc ggtccagcag
     1081 gcctgggatt tgccggtcca gcccttggcg agcgggccgc tgcgcagagg cgtgatattg
     1141 gcgcgccggt cggcgaagcg ggcgatgatt tcgcgagtgc cgtcgttcga attgtcgtcg
     1201 acggcgagaa tgcgcaggcg agccggcgga taggtctggg cggcgagccc cgccaggcag
     1261 gcgcggatgt tcgctgcttc gttgcgcgcc ggcagaatga cggcgacttt cggcgcgtcg
     1321 tcgccgaaga cggcgcgctt cggctccaga acttgcaaag cgcgcgactg gcgaacggcg
     1381 cgcaggagaa gaagaaacac aaccaggagg agagtggagg aggctaggag ctccacttgg
     1441 caaccatttt cgcgcgacgg gtttgaggca tatttccgcc aattaaaccc cgcggcgacg
     1501 acgcttgttc ccgttttacg ccgcctgcct gcgaattatc accgtcccag gcaaaagctg
     1561 ctgtcggacg gtattcgagc ttgcaagcgg cgcgtcaacg gcggctctgg ccggccttcc
     1621 ccatgaggcg aggcgcgatc ctcgctccgc gacgaaagag cggcggcttg gctacatctc
     1681 gccggattgc ggccggtcga cgaggggaga aacggcgaag gccgccacgc ccatggcgag
     1741 ggcggaaaag accagcaacg cggccatgac cagacctctc tcgccccccc gcatccaagt
     1801 ccgcggcgcg gcgattggca atcggcccgt ccgcttctcg gtgcgcggtc gtaccgtgtt
     1861 ttgcttcgga cctgcgcgcc ttgttcctct atcgggcgtt atggtcttgc tcgcgacgcg
     1921 ccccctcgct tgcgagcttt tggcggagcc ctccttcctc tgtggatggg cgaccgcctc
     1981 cgcgcgagag gcgagcctcc gccgcgcggc gtgtcggccc cgttaagctg cttggcgggg
     2041 ccgacatcat ttccgagccg gctcatcgaa agcatgcggc gcagccgttt tgagttggtc
     2101 ccgaccgcgc ctgctcgcgc catgctttga ggaaggcaaa gcgccgccgc attcctcgcg
     2161 catagggcgc ttagatcgtt tctggcgagg agacggacat gaaatccaac gcaacgatgc
     2221 tcaacgcctt gacggggcca tgagcggatg gcgggccgct gctcgatcgc gttcgcccag
     2281 ggcgctcgat ttctcggctg gctattctgt cttgcggcga ttgccgccct tgcgctctcg
     2341 gcgacgggct ccggctttgc aagcggccgc ccgtgggacg atcgcgcctt gccggcgtcc
     2401 cagcgagccc aattggcgct caatgagttc tcgcaggaag agaaactctc gctgacccat
     2461 ggcgggctcg gcgcgccctg gggcgacgag ccaaagcctg ccggcgcggt cggctcggcc
     2521 ggatacgttg ccggaattcc gcggctcggc gttcccgcgt tgcaggagac ggacgcggag
     2581 ctcggcgtcg ccaatccggg caatatccga ccgggcgaca cggcgaccgc gatgccctcc
     2641 gacctggcgc tcgcttcgac atgggatgag gcgctcgcgc ggcggcaggg cgagaccgtc
     2701 ggcgcggagg cccgcgccag gggtttttcg gtcttgctcg gcggcgcggt caatctcatc
     2761 cgcgacccgc gcggcggccg caatttcgag tatttctccg aggacccgtt gctctccgga
     2821 acgatggccg gcgcggcggt cgccggcgcg cagagccggc atgtcatttc gaccgtcaag
     2881 cacttcgccc tgaacgacca ggaaaccgac cgcgtcgtgc tcgacgccga gatcgaccgc
     2941 gcggcggcgc gagaatcgga tcttttcgcg ttcgaaattg cgctggagcg gagccgcgcc
     3001 ggcagcgtga tgtgcgccta caatcaggtc gacggcgcct attcctgcga gaacgattgg
     3061 ctgctgagcc aggtgctcaa gggcgattgg cgttatccgg gattcgtgat gtcggattgg
     3121 ggcgcggtcc attcgaccgt tgcttcggct ttggccgggc ttgaccagga gtcgggcgag
     3181 caactcgaca cgcagaactt ttttgatcgc cctttggcga aagccgtcga agacggcgcc
     3241 gtgccgcaag cgcgattgga cgatatggcg cggcgcattc tgacctcgat ctttgcctcc
     3301 ggcctctacg acgatccgcc gcggcgcggt ccgatcgacg cggcggccgg cgatcgcacg
     3361 gctctggaga tcgcgcgcga aggcctcgtt ctgttgaaga atgacggcgt tctgccgctc
     3421 gccgggagcg tgcggcgatt ggcggtgatc ggcggccacg ccgacaaggg cgtgctgtcg
     3481 ggcggcggct cgtcccaggt cgtcccgcgc ggcggcgtcg cgctcgccga gccgagcacg
     3541 cgcgtcgagg gcgacctgat cttcgatccg tcttcgccgc tcgaagcgct gcgccgccaa
     3601 ttcccgcaag cccaaatcgt ctatgacgac ggtcgcacgc ccgccgaggc ggcgaaactg
     3661 gccgcgacgg ccgacgcggc gatcgtcttt gcgtatcaat ggatgacgga gaccgccgac
     3721 gcgcccgatc tcgccttgcc gcgtgctcaa gatcggctga tcgcggcggt cgccgccgcc
     3781 aatccgcgca tgatcgtcgt gctcgaaacc ggtggcccgg ttcttatgcc atggcttggc
     3841 cgcgccgccg cggtgctgga ggcctggtac cctggccaga aaggcggcga ggcgattgcg
     3901 gaagcgcttt cgggcgcgat tgacccttcc gggcgttcgc ccgtcacctt cccgcgaagc
     3961 gaggcgcaat tgccgcgacc gacagtcccc ggcgatccca atgcggcgcc gcgcgggcca
     4021 gtcgggcgcg gcggtcatta tgggcggatt ttcaccgccg cttacaatga gggcgcggcg
     4081 gtcgggtaca aatggttcgc gcaatgccgg gaggcgccgc tcttcccatt cggcttcggc
     4141 ctctcctata cgcgctttgc tctaagcgat ctgaccgcgt cggcggacgg cgacgccgtc
     4201 acgatcagcg cgcaggtgca gaacatcggc gcgcgcgccg gcgcggcgat tccgcaattc
     4261 tatctctccg gcccgtcggg cgcggccatt cccttgcggc ttgcgggctg gagcaggatc
     4321 gatttgcggc ccggcgaggc gaggcgcgcg acaatcgccg tcgatccgcg gctgttcgcg
     4381 catttcgacg agagcgggcg gcgttggcgg atcgcgcctg gcgtctatcg catcatggct
     4441 ggtttcgacg cggcgcatcg cgagcaggcg accgagatcg gcctcaaagg agcggacttg
     4501 ccgccatagc cgagagtccg aaaagggccg cttcctttgt cggaagcggc ccagttggac
     4561 acggacccga ttaacggcgc gtcggatcag ttcaggttgc tcgccacttc attgaaggtc
     4621 gcggtgagct tcgttccgac gacggaaacc gctccgatga tcacgaccgc gatcagggag
     4681 gcgataagcc catattcgat agcggtcgcg ccatcctcgt tgcttaggaa tttcttaata
     4741 taaagtcgca tcagacagcc cccccctttt tgagacgccc atctatttgc gcttaaaaac
     4801 ttagagtacg tgaatttacc ccgtaagttt tccggttcgg aggcaaaatc gcgacggtgt
     4861 gatgataaat tattttttag agagccgctc atccgagcgc ggcgcgctat tgcttgatga
     4921 cgagcccgtc gacgaagacg tcagtgaggc gcgtcacggt cgcttgccaa ccgggctgat
     4981 cgtaagaata gcagaggccg atcaacgtac gaaaaaggtc ttcgggggtg acgtcggcgc
     5041 gcatttcgcc tgtcgcgacc gctcggttca ataaaaaccc gagcgatttg gtgaggcgcg
     5101 cgaaagaata agcatagagg tccggcggct tatgggcggc gagcgccagg gccgcgagca
     5161 ttcccttctt cgtcgcgacc agt
//
LOCUS       DATGJW010000085         3228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_118962, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000085 DATGJW010000000
VERSION     DATGJW010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3228
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118962"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1205)
                     /locus_tag="VKU03_02555"
     CDS             complement(<1..1205)
                     /locus_tag="VKU03_02555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ70170.1"
                     /translation="MAACFAGLAATAQAAEVKEVQMLHWWTSGGEAAALNVLKQDLAK
                     EGYAWKDVPIAGGGGEGAMTALKAMVAAGNPPTASQILGYFAIDYAEAGKLADITPLA
                     KKEGWDKVVPTALQKFTTTNGKWDAVPVNIHSVNWVWINKAVMEKIGGTEPKNFDEFI
                     ALLDKAKKAGVIPLALGGQPWQEATMFDSVVASTGGIDFYKKAFIDMDESALKSDTMK
                     KAFDNLAKLRDYVDPNYAGRDWNLATAMVIKGDALVQVMGDWAKGEFRAAHKEAGKDF
                     LCYRFPGTDGDVIYNTDMFAMFKVSADRQAAQLALAKATMSPSFQSAFNVVKGSVPAR
                     MDVPDTAFDMCGKKGIADVKAANDKGTFVGSMAQNYAQPPAVAGAYHDVVTKFFHGEI
                     KSSDAAVTEL"
     gene            complement(1419..2192)
                     /locus_tag="VKU03_02560"
     CDS             complement(1419..2192)
                     /locus_tag="VKU03_02560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004677682.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLJ70171.1"
                     /translation="MNAYVQLAFDGPVASITLNRADKLNALDRAMVVALAEAAEAIEA
                     SREARVAILSGEGKAFCAGGDIAAWSGLPPLDMWRDWTRLGHRAFEKLARLRQPLIAV
                     LAGHAFGGGLELAAAADIRIAERGIKLGLPETGLGMAPGWSGTQRLARRFGANVVRRM
                     ALGGATFTAEEAHGLGLVDEVVEPGAARQRAQALATAIAGRGPLAVQMVKAMINSAEG
                     EDRDAPIEGLAGALTAMTEDLAEGVAAFRGKRRAKFAGR"
     gene            complement(2189..>3228)
                     /locus_tag="VKU03_02565"
     CDS             complement(2189..>3228)
                     /locus_tag="VKU03_02565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HLJ70172.1"
                     /translation="NLSYEHEGGYLGPLDQALAARNNGGIVIAQVKRLAKAGTLKPQR
                     VFVPGILVDAIVVAPDQMQTTQTQYEPAISGEIFRPLCSFETPAFGVAKVIARRVAQE
                     LHQGDAVNIGFGISANVPRILIEEGLHGAVTWVIEQGAVGGVPLLDFQFGCASNAEAI
                     VPSPYQFTYFQGAGFDCSLLSFLEIDRDGSVNVSKLGTRPHVTAGAGGFVDITARAKK
                     IVFSGYFTAGAGLAIADGGLAIEKEGKVKKLVEKVEQVSFSGRRAKAQGQEIVYVTER
                     CVMALARDGVTVTEIAPGIDLKRDILEQAAFPLRVAPALKTMDAALFRPEPSGLRLKA
                     APPHAAREAAE"
BASE COUNT          514 a         1100 c         1054 g          560 t
ORIGIN      
        1 agttcggtga cggcggcgtc cgaagacttg atctcgccat ggaagaactt ggtcaccacg
       61 tcgtggtagg cgcccgccac cgccggcggc tgagcgtagt tctgggccat ggagccgacg
      121 aacgtgccct tgtcgttggc cgccttgaca tcggctatgc ccttcttgcc gcacatgtcg
      181 aacgcggtgt ccggaacgtc catgcgcgcc ggcaccgagc ccttgacgac gttgaacgcc
      241 gactggaagc tcgggctcat cgtcgctttg gccagcgcaa gctgcgccgc ctggcgatcg
      301 gccgagacct tgaacatcgc gaacatgtcg gtgttgtaga tcacatcgcc gtcggtgccg
      361 gggaagcgat agcaaaggaa gtccttgccg gcctccttgt gggcggcgcg gaactcgccc
      421 ttggcccagt cgcccatcac ctgcacgagc gcgtcgccct tgatcaccat cgcggtcgca
      481 aggttccagt cgcggccggc atagttcgga tcgacatagt cgcggagctt ggcgagattg
      541 tcgaacgcct tcttcatcgt atcggacttc agggcgcttt catccatgtc gatgaacgcc
      601 ttcttataga agtcgatgcc gccggtggag gcgacgaccg aatcgaacat cgtcgcttcc
      661 tgccagggct ggccgcccag cgccagcgga atcacgcccg ccttcttcgc cttgtcgagc
      721 agggcgatga attcgtcgaa attcttcggc tcggttccgc cgatcttctc catcacggcc
      781 ttgttgatcc agacccagtt gaccgagtgg atgttgaccg gaacggcgtc ccacttgccg
      841 ttggtcgtgg tgaatttctg caaggcggtc gggacgacct tgtcccagcc ttcctttttc
      901 gccagcgggg tgatgtcggc gagcttgccg gcctcggcgt agtcgatggc gaaatagccg
      961 agaatctgcg aggcggtggg cggattgccg gccgcgacca tcgccttcaa ggcggtcatc
     1021 gcgccctcgc cgccgccgcc ggcgatcggc acgtccttcc aggcgtagcc ttccttggcg
     1081 aggtcctgct tgaggacgtt cagcgcggcc gcttcgccgc ccgaagtcca ccaatgcagc
     1141 atctgcactt ccttgacttc ggcggcttgc gccgtggcgg ccaggcccgc gaagcacgcg
     1201 gccatcgcgc cggccaggag ctttgtacga agcttcattc gtctaccctt ccaaaatgcg
     1261 tatcgtccca accggattcg gtccggcgcc gtctccaaga accggtcaat cggtcccggt
     1321 ctccctgcca cgcctcagcg cccggcagaa tcgccgatcg cttttggaca tggaagctct
     1381 ctcctcagcg ccggcgtttc cggcgctcac gaatctcctc accgccccgc gaattttgcc
     1441 cggcgcttgc cgcgaaaggc ggcgacgcct tcggccagat cctcggtcat cgccgtcagc
     1501 gcgccggcaa gaccctcgat cggcgcgtcc ctgtcctcgc cttcggcgga attgatcatc
     1561 gccttgacca tctgcacggc gagcggcccg cggccggcga tcgcggtcgc cagcgcctgc
     1621 gcccgctggc gggcggcgcc cggttcgacc acctcgtcga cgaggccgag gccatgcgcc
     1681 tcctccgccg tgaacgtcgc gccgcccaag gccatccggc gcacgacatt ggcgccgaag
     1741 cgccgcgcca agcgctgcgt gcccgaccag cccggggcca tgccaagccc ggtctcgggg
     1801 aggccgagct tgatcccgcg ctcggcgatg cggatgtcgg cggccgccgc aagctccagg
     1861 ccgccgccaa aggcgtggcc ggccaagacg gcgatgagcg gctggcgcag ccgcgccagt
     1921 ttctcgaaag cgcggtggcc gagccgcgtc cagtctcgcc acatatcgag cggcggcagg
     1981 ccgctccagg cggcgatgtc gccgccggcg caaaacgcct tgccctcgcc ggaaaggatg
     2041 gcgactcgcg cctcgcggga ggcctcgatc gcctccgccg cctcggcgag cgcgacgacc
     2101 atggcgcggt cgagcgcatt gagcttgtcg gcgcggttaa gcgtgatcga tgcgaccggc
     2161 ccgtcaaaag cgagttggac gtaggcgttc attcggcggc ctcgcgagcg gcgtgcggcg
     2221 gcgcggcttt cagccgcaag ccgctcggct cggggcggaa cagggcggcg tccatcgtct
     2281 tgagcgccgg ggcgacgcgc agcggaaagg cagcctgttc gagaatgtcg cgcttcaggt
     2341 cgatcccggg cgcgatctcg gtcacggtca cgccgtcgcg cgccaaggcc atgacgcagc
     2401 gctcggtcac atagacgatt tcctggccct gcgccttggc gcggcggccc gaaaacgaca
     2461 cttgctcgac cttctcgacc agcttcttga ccttgccttc cttctcgatc gccagcccgc
     2521 catcggcgat cgctagcccc gccccggcgg tgaaatagcc ggaaaagacg atcttcttgg
     2581 cgcgcgcggt gatgtcgacg aagccgccgg cgccggcggt gacatgcggc ctggtgccga
     2641 gcttggagac gttgacggag ccgtcgcggt cgatttcgag gaaggacaaa agcgagcaat
     2701 cgaagcccgc gccctggaaa taggtgaact gataaggcga aggcacgatc gcctcggcgt
     2761 tggaggcgca gccgaactgg aaatcgagca gcggcacgcc gccgaccgcg ccttgctcga
     2821 tcacccacgt cacggcgccg tgcaggccct cctcgatgag aatgcgcggc acattggcgg
     2881 aaatgccgaa accgatattg accgcgtcgc cttgatgcaa ttcctgcgcg acgcgccgcg
     2941 cgatgacctt ggcgacgccg aaggcgggcg tttcgaagga gcagagcggc cggaaaatct
     3001 cgccggaaat cgccggctcg tactgggtct gggtcgtctg catctggtcg ggcgcgacga
     3061 cgatggcgtc gacgagaatt cccggcacga agacccgctg cggcttcagc gtgccggctt
     3121 tggccaggcg cttgacctgg gcgataacga tcccgccatt gttgcgcgcg gcgagcgcct
     3181 gatcgagcgg accgagatag ccgccctcat gctcgtagga gagattgc
//
LOCUS       DATGJW010000086         2889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_14646, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000086 DATGJW010000000
VERSION     DATGJW010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2889
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14646"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..472)
                     /locus_tag="VKU03_02570"
     CDS             complement(<1..472)
                     /locus_tag="VKU03_02570"
                     /inference="COORDINATES: protein motif:HMM:NF015763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HLJ70173.1"
                     /translation="MLKAIRRFFDESAPDLKARLFFICGALAALNIGAWLWALAAFRH
                     SPVPLGVALVVYGLGLRHAVDADHIAAIDNVTRKLMQDGQRPVSVGFFFAMGHSAVVI
                     FVTAAVVGASSMLEGVESLHGVGDMISTSVSAIFLLAIAAMNIVIFFSIYESYRR"
     regulatory      complement(570..769)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            1009..1659
                     /locus_tag="VKU03_02575"
     CDS             1009..1659
                     /locus_tag="VKU03_02575"
                     /EC_number="2.5.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
                     /protein_id="HLJ70174.1"
                     /translation="MQLIGMLDSPYVRRVAIALLRLGLPFEHRPLSLFRHIDEFRAIN
                     PLLKAPTFVADDGTVLVESTLIIEYAQGLAPRAPRLTPGAPATKLKSYRLSGLALTVN
                     EKAVQIHYERQLRPPEKQHEPWLARVRGQLEAGLEMLDRETAKTQNWLVGENLMLADI
                     TVATAFAFVQLNLADIAPAARYPALAAFCRRAEALTEFRAAPCQDGAKVAAPVIPA"
     gene            1693..2691
                     /locus_tag="VKU03_02580"
     CDS             1693..2691
                     /locus_tag="VKU03_02580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-quinone oxidoreductase"
                     /protein_id="HLJ70175.1"
                     /translation="MPGLPKTMKAVVAAQPGGPEVLQIGERPLPAPREGEILVEVAAA
                     GVNRPDVMQRQGHYPPPPGAPDILGLEIAGTVVALGAGAARHRLGDKVVALVPGGGYA
                     QYALAHESNALPIPAGLSMIEAAAIPETFFTVWTNVFEGGDLKAGETLLVHGGTSGIG
                     TTAIMLAKAFGARAIATAGSRDKAAACLRLGANVAIDYRAADFVAETLKATDQKGADV
                     ILDMVGGDYVARNYAAAAKGGRIVQIALLHGSKATLDLLPLMQKRLIHTGSTLRPRKP
                     EEKALIADALKEKVWPLLAAGRCKPVIDSTFPLEDAAKAHARMESSAHIGKIVLTI"
     gene            2723..>2889
                     /locus_tag="VKU03_02585"
     CDS             2723..>2889
                     /locus_tag="VKU03_02585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HLJ70176.1"
                     /translation="MAIPLMPKATAVWLVENTSLSFDQIGEFCGLHPLEVQAIADGEV
                     AMQMQGLDPVA"
BASE COUNT          506 a          965 c          943 g          475 t
ORIGIN      
        1 cccgccgata gctctcatag atcgaaaaaa agatgacgat gttcatcgcg gcgatggcga
       61 gcagaaagat cgccgagacg ctcgtgctga tcatgtcgcc gacgccatgc aggctctcga
      121 cgccttcaag catgctcgat gcgccgacca cggcggcggt gacgaagatc accaccgccg
      181 aatggcccat ggcgaagaag aaaccaaccg agaccggccg ctgtccgtcc tgcataagct
      241 tgcgggtgac gttgtcgatc gccgcgatat ggtccgcgtc gacggcatgg cgcaggccga
      301 ggccgtagac gaccagcgcg acgccgagcg ggacggggct gtggcggaag gcggcgagcg
      361 cccacagcca ggcgccgata ttcagcgcgg ccagcgcgcc gcaaataaag aaaaggcgcg
      421 ccttcaagtc gggcgcgctc tcgtcgaaaa atcgtcggat ggctttgagc atggcggaac
      481 cccgcgcgac gtcgcttgtc atcgcagagg gttgaccgcc tttcttaagg cggcttcgcc
      541 gcgccgagac accccgctcg acgctcccgc gctgacgtcc ttttgatggc aggtctcctg
      601 gctcgcggtt tggcgccgct tccgccttcc cggggccatg cgtcccagtg gcgagaagga
      661 agcggctcgc cgcttacagt tgcgggggca gccgcggctt tggacgagag ccgtccgcac
      721 cgcgttccct tttgatccct agaagggagc catcgacgcg acgatagccg ctcaggcggc
      781 ggcgtcaagg tacgattccg cgccccgcga aacgcggggc tgcgctgcgc ttgctgactg
      841 gcgattcaca cggcgcgcct tccgaagtct tgcgcagccc tcatccggcg cgccccgtcg
      901 tctccgcttt gcggggggaa ggggtttcgc gcataataat cgcgcccatg caaggcgccg
      961 gccgagacgg cggcggctca aatctccgtc gatgaggatc gcgcaaagat gcaactgatc
     1021 ggcatgctcg attcgccata tgtccgccgc gtcgccatcg cgctgttgcg gcttggcctg
     1081 ccgttcgagc accggccgct gtcgctgttc cggcatatcg acgaattccg cgcgatcaac
     1141 ccgctgctca aggcgccgac tttcgtcgcc gacgatggga ccgtgctggt ggaatcgacg
     1201 ctgatcatcg aatatgcgca gggcttggcg ccgcgcgcgc cgcgcctgac gcccggcgcg
     1261 ccggcgacaa agctgaaatc gtaccggctg agcgggctcg ctctgaccgt caacgaaaag
     1321 gccgtgcaga ttcattacga gcgtcagctc aggccgccgg aaaagcagca tgagccgtgg
     1381 ctcgcccggg tgcgcggaca attggaggcc ggccttgaaa tgctcgaccg cgaaaccgcc
     1441 aagacgcaga attggctggt cggcgaaaac ctgatgctcg ccgacatcac ggtcgcgacc
     1501 gctttcgcct tcgtccagct caatctcgcc gatatcgcgc ccgccgcgcg ctatcccgcc
     1561 ctcgccgcct tctgccgccg cgccgaggcg ctgacggagt ttcgcgccgc gccttgccag
     1621 gacggcgcga aagtcgccgc gccggtcatt ccggcttaaa gtttcgcgcg cggtccgaag
     1681 agagagattg tcatgcccgg tctgccgaaa acgatgaaag ccgtggtcgc cgctcagcct
     1741 ggcggcccag aggtccttca gatcggcgag cggccgcttc ccgcgccccg cgagggcgaa
     1801 attctcgtcg aggtcgcagc ggctggcgtc aaccgcccgg atgtgatgca gcgccagggc
     1861 cattatccgc cgccgcccgg cgcgcctgac attctcggcc ttgaaatcgc agggacggtc
     1921 gtcgcgctcg gcgccggcgc ggcgcgccac aggctcggcg acaaggttgt cgcgctcgtg
     1981 ccgggcggcg gctacgcgca atatgcgctc gcgcatgaga gcaatgcgct gccgattccc
     2041 gccggccttt cgatgatcga ggccgccgcg attccggaga catttttcac cgtctggacc
     2101 aatgtcttcg agggcggcga cctcaaagcc ggcgagaccc tgcttgtcca tggcggaacc
     2161 tccggcatcg gcacgacagc gatcatgctc gccaaggcgt tcggcgcccg ggcgatcgcc
     2221 accgctggct cgcgcgacaa ggccgcggcg tgcctacgcc tcggcgccaa tgtcgcgatc
     2281 gattatcgcg ccgccgattt cgtcgccgag accttgaagg cgacggacca gaaaggcgcc
     2341 gacgtcattc tcgacatggt cggcggcgac tatgtcgcgc gcaattacgc ggcggcggcg
     2401 aagggcggcc gcattgtcca aatcgccctt ctccatggat cgaaggcgac gctggatttg
     2461 ctgccgctga tgcaaaagcg cctgatccat accggctcga cgctgcgccc gcgcaagccc
     2521 gaggaaaagg cgctgatcgc cgacgcgctg aaagaaaaag tctggccgct gctcgcggcc
     2581 gggcgctgca agccggtcat cgattcgacc tttccgctcg aagacgcggc caaggcgcat
     2641 gcgcgcatgg aaagctcggc gcatatcggc aagatcgtgc tgacgatttg accctcggcc
     2701 ggcgaccgac tatagagccg cgatggccat accgctgatg ccgaaagcca ccgccgtgtg
     2761 gcttgttgaa aacacgtctt tgtcgttcga tcagatcggc gagttctgcg gactgcaccc
     2821 gctcgaggtg caggcgatcg cggacggcga agtggcgatg cagatgcagg gcctcgaccc
     2881 ggtggccaa
//
LOCUS       DATGJW010000087         7311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1052496, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000087 DATGJW010000000
VERSION     DATGJW010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7311
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1052496"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..190)
                     /locus_tag="VKU03_02590"
     CDS             complement(<1..190)
                     /locus_tag="VKU03_02590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="two-component system sensor histidine
                     kinase/response regulator"
                     /protein_id="HLJ70177.1"
                     /translation="MERSSPTILYVDDDEALVRLVQRGLSRRGYSVEGAATIEDGLAR
                     LGKGDIDVVALDHFLASGT"
     gene            complement(193..639)
                     /locus_tag="VKU03_02595"
     CDS             complement(193..639)
                     /locus_tag="VKU03_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013946498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ70178.1"
                     /translation="MKKEARPVVIVMIEDDEGHARLIEKNIRRAGVNNEIVPFTNGAD
                     ALAFLLGPDRSGEASAGRQLLVLLDLNLPDMTGIDILKELKENDHTRRSPVVVLTTTD
                     DSREIQRCYDLGANVYITKPVDYDGFANAIRQLGLFFAVMQVPEAD"
     gene            complement(655..2193)
                     /locus_tag="VKU03_02600"
     CDS             complement(655..2193)
                     /locus_tag="VKU03_02600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHASE3 domain-containing protein"
                     /protein_id="HLJ70179.1"
                     /translation="MDRLNAPASFATSRLIRLPRSIAAPVSKTTFFRLSIMMIGVGFL
                     ALLCIVCGAIWLVDRAERYSDALQVARQQSGALTRLLSLAQDAETGQRGYLLAGTDPY
                     LKPYQEATSSIPMVLDNLAPAIGSDAAQRRRFDELSAVLTKKLAELEDTVNLARAGRR
                     DEALQLLRTDKGRELMERLRSVISEMLGDADVRVANALNNERDAIAFMWRLVVVGALV
                     VVLVGAVVLWMGIAYTRQLDRNRSEIQTLNASLEKRVEERTADLARANEEIQRFAYIV
                     THDLRAPLVNIMGFTSELDASLDAIRTYVEAPEITAIVPAEVKQAAAEDLPEAIGFIR
                     SSTRKMDGLINAILKLSREGRRELKPERITLDALLRAIADSMQHQIMEAGGEVVIEGR
                     VSPIVTDRLAVEQIFGNLLDNAVKFRRFDTPLAIRIRLSDERGQRVSVTVEDNGRGIA
                     PQDFERVFDLFRRAGAQDRPGEGMGLAYVRNTTRNIGGDIKLQSELGRGTTFIVTLPR
                     VLRS"
     gene            complement(2563..3582)
                     /locus_tag="VKU03_02605"
     CDS             complement(2563..3582)
                     /locus_tag="VKU03_02605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70180.1"
                     /translation="MTGPRALSDWAQANRWIWSALAVLISWALLSAITDRFSIASLSG
                     ILLSASFLSIVGVGQMFVVAAGRGNIDLSIASVVTLSAYLALLTVRGQDANLAWGVLT
                     ALGLGLVVGAFNAALVVGLRVPAIIATLATGYVLATATLLSNRAMHGFAVSPTLREIA
                     AGRVLGAPIMLLLCLTLIAVSEIALRFTAFGKTLSAVGQNRTAAALAGVRVARVVAAT
                     FVISSVLASLMGLVLGAYVGGAFLEMGQAYFLQSVAAVVVGGTLILGGYATAIGVFCA
                     SVLLILIVTLMQIMQLPPGSQDMVQGVVVILVLALAGREALRRRADSDRSGREAPAET
                     SPAPP"
     gene            complement(3579..6053)
                     /locus_tag="VKU03_02610"
     CDS             complement(3579..6053)
                     /locus_tag="VKU03_02610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896809.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ70181.1"
                     /translation="MSDRLVVSRTVDEAGDLAQQPSLVELKGITKAFGPTLANANIDL
                     TVDRSDVIGLVGGNGAGKSTLMRILCGITAPTLGRIAFEGVDEPFAAYDTAAAQRRGV
                     RMVHQELSLCANLSVAENFFIEAPERAKARPGWRRVYRAAAREALDAVFPGNGIDVDA
                     IVDRLSIGERQMVEIARATATVNVRLIVLDEPTSSLDLERSRQLRDYIRARSRSGFAF
                     IFISHKLQEVVDVASRVVVLRNGAVAWQGAVAQSSVSRLVELMGGDASAAHHRAERAA
                     SLEDEVVRIGGAFAAEIDGEISLRRGEIVGLAGLEGHGQKELLRAIYEAGAREKGGVR
                     RVQPASYVSGDRQKEGVFPLWSVLANIAIGRIARRAALGLALETRERIAAAPAAERLR
                     LDAGRFPSRILELSGGNQQKALLSRAVAAETPIVLLDDPTRGVDIATKQDFYRLCAEL
                     AREGRTLIWRTTEDAELLACDRVLVLANGRVVRELSGPGLSEEAIVAASFSHIATSDA
                     HDARPLRRPQPLRRLVDAAPFIGLALVLAIMTAANPSVASVFGIDLLLMPALSLVLVT
                     EAQMFVVGGSEIDLGVGAFAGLISVVAATWLYDKPWTGAAAIIAALLAYAAIGAIIQL
                     RKIPAIVVTLGASFIWAGIGYSIQPTPGGTSPDWLTALTSWSLGPDIPTSVILIAAVA
                     ATGLILDRAPLGVALRGFGGNPAAMAASGWSPLRFALIRYLLAGLFSGAAGLSLTAIN
                     SSSDINSGNSYTLLSVAAVVMGGCALAGGVVSPIGAVVGAVTLALIGALLGALGVSSD
                     YNAAAQGLVLIALLAMRSTADRGDES"
     gene            complement(6121..7140)
                     /locus_tag="VKU03_02615"
     CDS             complement(6121..7140)
                     /locus_tag="VKU03_02615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ70182.1"
                     /translation="MKSSVALLAAAAAFLGFAAPAEASPKAPVVALSNAYYGNTWRHQ
                     MVEAFEAAAKQAKAEGKIADYIVMNGDGSVAQQNSQIAELILKKIDVLLVDAASETAV
                     NGVIEKACKAGIIVISFDSIANAPCNYQLNFDFKGYKAEQAEAVVKMLHGKGNVIQVR
                     GVKGSAPDNDMYNAQVSVLKKYPDIKVVATVYGQATASVAQAAIANVLPSLPHVDAVF
                     AQGGSDDIGIAQAFTQYDGEYTNHMPIIEGGGGTDFIKWWAEQNKKNGYQTISMNTTP
                     GIGGAALWLGVSLLEGAKAPKLMIMPVATVDASNLAEYAKLPPGQIISPTYSLDWVKQ
                     HLLAK"
BASE COUNT         1303 a         2399 c         2380 g         1229 t
ORIGIN      
        1 cggtgccgct cgccaggaaa tgatcgagcg cgaccacgtc gatgtcgcct ttgccgagcc
       61 gcgccaaacc atcttcgatg gtcgcggcgc cctcgacgct gtagccgcgc cggctcagtc
      121 cgcgctggac taggcggacg agagcctcat cgtcgtcgac atagagaatc gtcggactcg
      181 accgctccat gctcagtccg cttccggaac ttgcatgacg gcgaagaaca ggccgagttg
      241 tcgaatcgcg ttcgcaaagc cgtcgtagtc gacgggcttg gtgatgtaga cgtttgcgcc
      301 aagatcgtag cagcgctgga tttcgcggct gtcgtccgtg gtcgtcagca ccacgacagg
      361 cgagcgccgg gtatggtcgt tctctttcag ctccttcagg atatcgatgc cggtcatatc
      421 cgggaggttc aaatcgagca ggacgagcaa ttgccgtccg gcgctcgctt ctccagaccg
      481 gtcggggccc agcagaaaag ccagcgcgtc cgccccgttg gtgaacggaa cgatctcatt
      541 gttgacgccg gcgcggcgga tgtttttctc gatcagccgc gcatgcccct cgtcatcctc
      601 gatcatgacg atgacgaccg gccgtgcctc tttcttcatc acacagactc cactttacga
      661 gcgcaagacg cgaggcagcg tgacgatgaa ggtcgtcccg cgccccagtt ccgattgcag
      721 tttgatatcc cctccaatgt ttctcgttgt gtttcggaca taggccaatc ccatcccttc
      781 cccgggacgg tcttgggcgc cggcgcgccg aaagagatcg aacacgcgct cgaaatcctg
      841 cggcgcgatg ccgcgtccgt tatcctcgac ggtcacgctg acgcgctgcc cgcgctcgtc
      901 gctgaggcga atgcgaatgg ccaagggcgt gtcgaaccgg cggaacttca cggcgttgtc
      961 gagaagattg ccgaatatct gctcgaccgc gagccgatcg gtcacgatcg ggctcacacg
     1021 gccctcgatg acgacctcgc ctccggcctc catgatctga tgctgcatgc tgtcggcgat
     1081 ggctctgagc agcgcgtcga gcgttatgcg ctcaggcttc aattcccgcc ggccctcacg
     1141 cgagagcttc aggatcgcat tgatcaatcc atccattttc cgggtcgacg agcggatgaa
     1201 tccgatcgcc tccggcaagt cctccgccgc cgcctgtttc acttccgccg gaacgatcgc
     1261 ggtaatttca ggcgcctcca cataggttcg gatcgcgtcg aggcttgcgt cgagctcgct
     1321 cgtgaatccc ataatgttga ccagcggcgc ccgcaaatcg tgtgtcacga tataggcgaa
     1381 gcgctgaatt tcctcgttgg cccttgcgag gtccgccgtg cgctcttcga cgcgtttctc
     1441 gaggctcgcg ttgagcgtct ggatttcgga tcggttccgg tcaagctggc gggtataagc
     1501 gatccccatc cacagcacga cggcgccgac caagacgacg acgagcgcgc cgaccacgac
     1561 gagccgccac atgaacgcga tcgcgtcgcg ctcattgttg agcgcgttgg cgacgcgaac
     1621 gtcggcgtcg cccagcatct cggatatgac gctgcgcagc ctctccatga gctcccggcc
     1681 cttgtccgtc cgcaggagct gaagagcttc gtcgcgccgg ccggcgcgcg ccaagttcac
     1741 cgtgtcttcg agctcggcaa gcttcttggt caggaccgcc gacaattcgt caaatcgccg
     1801 ccgttgagcg gcgtccgagc cgatggcggg cgcgaggttg tcgaggacca tcggaatgct
     1861 cgacgtggcc tcctgataag gcttcaggta gggatcggtc cccgccagca ggtagccgcg
     1921 ctggccggtt tcggcgtcct gggcgaggct aagcaggcgc gtcagagcgc cgctctgctg
     1981 tctcgcgact tggagcgcgt ccgaatagcg ctcggcgcgg tccaccagcc aaatcgcgcc
     2041 gcacacgatg cagagcagcg cgagaaatcc cacgccgatc atcatgatcg aaagccggaa
     2101 aaacgtcgtc ttcgaaaccg gcgcggcgat cgatcggggc aggcgaatca agcgtgacgt
     2161 tgcgaacgag gccggcgcgt tcaggcggtc cattgcgcag cctcgaacgg tatgctccgg
     2221 gagtcgtcgc tctgggcgag cgaggcgcta tgggtgatcg gcatgcctgt cccttgcata
     2281 ggcggagctc atcggcggcc gcccgctaaa atgcgcaaaa cgggagaatg cggccggctg
     2341 cggcgggccg cgcgcgcctg gagcgccggg gtctccgcca ttggacggct gtgtaacccg
     2401 gcatttcgcc aatacaagcc ttttgcaatt tccgccgtca gatgtctgtt cgaacgccgt
     2461 tctcatccgg ctccggccgg cggataatga taccggcgcc ccgcggattc gtcatcaccc
     2521 gccggccggc aagcccgttg tgtcgccgga gcatggcggc cgtcagggcg gtgcggggct
     2581 cgtctcggcc ggcgcttccc ggccggagcg gtccgagtcg gcccttcggc gcagcgcctc
     2641 ccggccggcg agggcgagga cgagaatcac cacgacgccc tgcaccatat cctgcgaccc
     2701 tggcggcaat tgcatgatct gcatcagcgt gacgatcagg atcagaagga cgctggcgca
     2761 gaacacgccg atcgcggtcg cgtagccgcc gaggatcagc gtgccgccga cgacgacggc
     2821 ggcgaccgac tgaagaaaat aggcctggcc catttcgagg aacgccccgc cgacataggc
     2881 gccaagaacc aggcccatca gcgacgccag caccgaggag atcacgaagg tcgcggcgac
     2941 gacccgcgcc acgcgcacgc cggcgagggc ggcggcggtt ctgttctggc ccacggcgct
     3001 gagcgttttg ccgaacgcgg taaaccgcag cgcgatttcg ctgacggcga tcagcgtcag
     3061 gcaaagaagc agcatgatcg gcgcgcccaa aacgcggccc gccgcgatct cgcgcagcgt
     3121 cgggctcacc gcgaaaccgt gcatcgcgcg gttgcttagg agcgtcgccg tcgccagcac
     3181 atagccggtc gccaaggtcg cgatgatcgc cggcacccgc aggccgacga cgagagcggc
     3241 gttgaaggcg ccgacgacga gccccagccc aagcgccgtc agcacgcccc aggcgagatt
     3301 ggcgtcctgg cccctcaccg tcagcagcgc cagataggcg ctgagggtga cgaccgaagc
     3361 gatcgaaaga tcgatattgc cgcgcccggc cgcgacgacg aacatctggc cgacgccgac
     3421 gatcgacaga aaagaggccg agaggaggat tcccgaaagc gaggcgatgc tgaaccgatc
     3481 ggtgatcgcc gacagaagag cccaggagat cagcaccgcc agagccgacc agatccagcg
     3541 attcgcctgg gcccagtccg acagcgctcg cggcccagtc atgattcgtc tccgcgatcg
     3601 gcggtcgagc gcatcgcaag cagggcgatg agcacaagcc cttgcgccgc ggcgttgtaa
     3661 tcgctgctga cgccaagcgc gccaagcaag gcgccgatca gtgcgagcgt caccgcgccg
     3721 accacggcgc cgatcggcga tacgacgccg ccggccaggg cgcagccgcc catcacgacg
     3781 gctgccacgc tgagcagcgt gtaggaattg ccggaattga tgtcgctcga agaattgatc
     3841 gccgtcaggg aaaggccggc ggcgcccgag aacaggccgg cgagaagata gcggatcagc
     3901 gcgaagcgca gcggcgacca gcccgaggcc gccatcgccg ccggattgcc gccgaagccg
     3961 cgaagcgcca cgcctaacgg cgcccgatcg agaatgaggc cggtcgccgc caccgcggcg
     4021 atgagaatga ccgatgtcgg aatgtccggg ccgagcgacc agctcgtcag cgcggtcagc
     4081 caatcggggc tggttccacc gggagtcggc tgaatcgagt agccgatccc ggcccagatg
     4141 aaagacgcgc cgagagtcac cacgatcgcc gggattttgc gaagctgaat gatcgcgccg
     4201 atcgccgcat aagcgaggag cgcggcgatg atcgccgccg cgcccgtcca aggcttgtcg
     4261 tagagccacg tcgcggccac gacgctgata agcccggcga aggcgccgac gccgagatcg
     4321 atctcgctgc cgccgacgac gaacatctgc gcttccgtca ccagcaccag ggagagcgcc
     4381 ggcatcagca agaggtcgat gccgaagacc gaggcgaccg agggattcgc cgccgtcatg
     4441 atcgcaagga caagagcgag gccgatgaac ggggcggcgt cgacgagccg gcgcaagggc
     4501 tgcgggcgcc gcagcggccg cgcgtcatgc gcatcgctcg tcgcaatatg ggagaaggac
     4561 gccgcgacga tcgcctcctc ggaaagacca ggcccgctca gctcgcggac cacccgacca
     4621 ttggcgagga caagcacccg gtcgcaggcc aggagctcgg cgtcctccgt ggtgcgccag
     4681 atcagggtgc ggccttcgcg agccagttcg gcgcaaagcc ggtagaaatc ctgcttggtg
     4741 gcaatgtcga cgccgcgggt cggatcgtcc agcaggacaa tcggcgtttc ggcggcgacc
     4801 gcgcgcgaca gcagcgcctt ttgttgattg ccgccgctca attcaagaat gcgggagggg
     4861 aagcggcccg cgtcgaggcg aagcctttcc gccgctggcg cggccgcaat tctctcgcgc
     4921 gtttccaacg ccaagccgag cgccgcgcgc cgagcgatgc ggccgatcgc gatgttggcg
     4981 agcacgctcc agagcggaaa tacgccctcc ttctgccggt cgccggagac gtagctggcg
     5041 ggctgaacgc gcctgacgcc gcctttctcg cgcgccccgg cttcgtagat cgcgcggagc
     5101 agctccttct ggccgtggcc ttcgagcccc gccaagccga cgatctcgcc gcggcgcaga
     5161 ctgatctcgc cgtcgatctc cgccgcaaaa gcgccgccga tgcgcacgac ctcgtcctca
     5221 agactcgccg cgcgctcggc gcggtgatgc gccgccgagg cgtcgccgcc catcaattcg
     5281 acgagccggc tcaccgagga ttgcgcgacc gcgccctgcc atgcgacggc gccattgcgc
     5341 aacacgacga cgcgcgaggc gacgtcgacg acttcctgga gcttgtggct gatgaaaatg
     5401 aaagcgaagc ccgatcggga tcgggcgcgg atatagtcgc gaagttgccg cgagcgctcc
     5461 agatcgagcg aggatgtcgg ctcgtcgaga acgatgagac ggacgttcac cgtcgccgtc
     5521 gcgcgggcga tctcgaccat ctgccgctcg ccgatcgaga gccggtcgac gatcgcatcc
     5581 acgtcgattc cgttgccggg gaaaaccgca tcgagcgcct cccgggcggc ggcgcggtag
     5641 acgcgccgcc agccggggcg cgccttggcg cgttccggcg cttcgatgaa gaagttctcg
     5701 gcgacggaca gattcgcgca gagcgagagc tcttgatgca ccatgcggac gccgcgccgt
     5761 tgcgcggcgg ccgtatcgta ggccgcgaaa ggctcgtcga cgccctcgaa cgcgatccgg
     5821 ccgagcgtcg gcgcggtgat gccgcagagg atgcgcatca gggttgattt gccggcgccg
     5881 ttgccgccga ccagtccgat cacgtccgag cgatcgacgg ttagatcgat attggcgttc
     5941 gccaaggtcg ggccgaacgc cttggttatc cctttgagct cgaccaggct cggttgctgc
     6001 gcgaggtcgc ctgcttcatc gaccgtccga gaaacgacga ggcgatcgga cactgaaatc
     6061 tcccaaagcg ccgtccggcg ccggtcgatc cggcgccgga cggtcggccg ctcagccgcg
     6121 ctacttcgcc agaaggtgct gcttgaccca gtccagcgaa taggtcgggc tgatgatttg
     6181 gccggggggc agcttggcgt attcggcgag gttgctcgca tcgaccgtcg ccaccggcat
     6241 gatcatcagt ttcggcgctt tagcgccttc gagcaaggag acgcccaacc acagggccgc
     6301 gccgccgatc cccggcgtcg tgttcatcga gatcgtctga tagccgttct ttttgttctg
     6361 ctcggcccac catttgataa aatcggtgcc gccgccgcct tcgatgatcg gcatgtggtt
     6421 cgtgtattcg ccgtcatatt gcgtaaaggc ctgggcgatg ccgatgtcgt cgctgccgcc
     6481 ctgggcgaag acggcgtcga catgcggcag gctgggaagg acgttggcga tcgccgcctg
     6541 cgcgaccgac gctgtggcct ggccgtagac ggtcgcgacc accttgatgt ccggatactt
     6601 tttcaggacg gagacttggg cgttgtacat gtcgttgtcc ggcgccgatc ctttgacgcc
     6661 gcggacctgg atcacgttgc ccttgccgtg cagcatcttg acgaccgctt cggcctgctc
     6721 ggccttgtag cccttgaaat cgaagttgag ctggtagttg cacggcgcgt tggcgatcga
     6781 atcgaacgag atcacgatga tccccgcctt gcaggccttc tcgatgacgc cgttcaccgc
     6841 cgtctctgac gcggcgtcga ccagcagcac atcgattttc ttgaggatca attcggcgat
     6901 ctggctgttt tgctgagcca ccgagccgtc gccgttcatg acgatgtagt cggcgatctt
     6961 gccctcggct ttcgcctgtt tcgccgcagc ctcgaacgcc tcgaccatct gatggcgcca
     7021 ggtgttgccg taataggcgt tcgagagggc gacgacaggc gctttgggac tggcttcagc
     7081 cggcgcggcg aagccgagga acgccgcggc ggcggcgagt aaggcgacag atgatttcat
     7141 tcgatctcct cccgattgga cgcagtcggc ggccgccgct tgaacgcggc atgtacacat
     7201 agtataccag tgccaaattt ggcaagcgct caattgaaaa cctagattga tctctgaaat
     7261 attgggcgga gttggctgct gggcgccttc gcccttcttc cgccctcatt c
//
LOCUS       DATGJW010000088        18367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_429034, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000088 DATGJW010000000
VERSION     DATGJW010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 18367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18367
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_429034"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..340
                     /locus_tag="VKU03_02620"
     CDS             <1..340
                     /locus_tag="VKU03_02620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70183.1"
                     /translation="EYGHFFEHNPIEIVNVRVTGVGAMPKIQKLRAPAGGSVAAATVR
                     AGQCMFRVDGEFRPFETTFYRRHLLPVGETISGPAVILQKDSTTVIPPRWSSLTDAAG
                     NLILKFDGDA"
     gene            340..2142
                     /locus_tag="VKU03_02625"
     CDS             340..2142
                     /locus_tag="VKU03_02625"
                     /inference="COORDINATES: protein motif:HMM:NF014585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLJ70184.1"
                     /translation="MQPFEGTNTPARLATRVDPITGAVIQGALENIAIEMGHKLMRMS
                     YSSIIRESEDFGAALTDAEGNQLCECKMSTPLQSGPIPGYVRGVKKRFAARGDVFRPG
                     DVIMHNDPYGGASHGPDVAFCVPVFDGETLLGFSVTTAHHLDIGSHTPGSCGIVDAVD
                     AYAEGLQFKAIKVVDQGKRNDMVWHMLRDNIRISDLVVGDMEAQIAAAQIGAQRFLDL
                     VRHYGRDTVNGASADLMDYSERLMRAAIGAIPDGVYSASTYIDGFLDDPEPSRRDLKI
                     VVTITVKGDEMLVDLAGTAPQVPDRPINMPFEGTVDCAIWLTLRSILLDSVVYGPIPQ
                     NSGLTRPIRIVAPKGCLANPVFPAPVIARFCPGNALADAVMKALAPAVPGQISAGIGN
                     LTVIAFSGVANDQQWVHMEILEGSYGGRRGMDGMDTVDTLYANTRNNPIEDIESHLPL
                     RVERYELREDVAAPGEWRGGVGSIREFRYLTDGGFSVEGDGHKFKPWGFDGGADGAPS
                     ALVQASSSGERIELPSKVPYRKAKAGDRLISLGPSGGGYGNPLARAPEAVLENVLDGI
                     ISADLARRQYGVAIANGEIDAAATEALRAKMRLR"
     gene            complement(2170..4341)
                     /locus_tag="VKU03_02630"
     CDS             complement(2170..4341)
                     /locus_tag="VKU03_02630"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLJ70185.1"
                     /translation="MRDEIARNIEVGKLRPGIVLLEGPIATLLGVSRWPVKRALELLA
                     DEGLIRRFRGRGYLVGGAGEDAAPLRVNLLALGLDVSGVIQDYAQRAAWQKIYGEAAE
                     SVVACMPFGAYKISEPAMCAHFDVSRTVVRDVLARLNHDGLIEKDRWSHWTAGPLTAR
                     DVKEHFEIRQLLEPAALRLAAKSLDRRTVETMRARIEQALQAAHSLTPREVEEIERDL
                     HEACFASVSNRRLTATIQQSRMPDVINRLFAKHFGLSEAMAALKEHAQVLDHLLAGEV
                     EKAAAALSFHIGRAMERTRARLKVLSVLTAQDVAPYLTPIVDGRSASHKSRALRADRK
                     RRPRKTRPALRVLGRREILLEPVRRQAAKDLGFDIEFELLEGAAEIRQAATRPDSFDV
                     YHQWHTVDLMWTARSIQPIAIERIPAWSEIASLASLSPDGATICDDLCRQLYVQDDGR
                     LASSPTRLASMLPTIHGTDSLGYLKNLREQLRPGERDSWAWLIDDRWRGKVAMLRDPT
                     LGMIEAALAMEGAGILKFADIANLSIEEIDAIIAILKDKKRNGHFRGLWETYEDAVRL
                     MERGGVVVQSIFSPAVIKLRRRGLDVMSAMPAEGSRGWHSDLCLSAAASGETLEAAYA
                     YLNWWMEGPAGAILARQGYYMSAPERTRRHLSEAEWDYWYEGKPARVDLPDPFGETCV
                     RAGEIRGGGSYIDRMSRVRVWNTIMDDHNYLARRWNEFLSA"
     gene            4805..6160
                     /locus_tag="VKU03_02635"
     CDS             4805..6160
                     /locus_tag="VKU03_02635"
                     /inference="COORDINATES: protein motif:HMM:NF012312.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70186.1"
                     /translation="MLSNPAAVSPGEARNPASPAIPDVSPLTKGQVAYTAVVALLAWT
                     FAVYDLITFGNLLPKIQQEFGWSNATASYVATLVGLASLVVALCVGPIIDGLGRRFAL
                     VLTTGGAAVSSGLAALAFGPVSLVVFRALSGFGMSEQAVNSAYLNEVFAARAKGFLYG
                     IVQAGWPLGVMLSSGLAALLLPEIGWRGVFLVAAFPLLVMLVCRFGLRESPYFLKVQH
                     LRRLRDRGDAAGAEELARRWSLDMTGDRWNTYAELFRPALRRQSIALGAMFFFKIIAD
                     SQLTVLATSVLGQAKGIDLTSALWTIFFGNGVAVLGYLFLGWTGDRIGRRETVIIAQT
                     LAAVCTFALLFLAHGLAGVIVCYALVLFFAQGAAAPFFAYVGESFPTRVRGSGAAYIN
                     VAGPIGGIFGPLVYGALQQAGASPTAAAASGGVAALVAAACMLAARSIRPGQELSDIS
                     H"
     gene            complement(6175..7230)
                     /locus_tag="VKU03_02640"
     CDS             complement(6175..7230)
                     /locus_tag="VKU03_02640"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HLJ70187.1"
                     /translation="MSGDERLRWIEAYEATGRAGFVCAKFGISRPTLRKWWRKYKEFG
                     PAGLTDSSRRPVSSPLQKVFAREEALILALRKSENLGVHRLRSELRRRHGLNLSPETI
                     LKVLKRAGEPMKTKRSEAARETAAPDPAPIRQFGHGGLFHGLASDDRVAHEIASLIVK
                     ERFRPGQKLSEAALGAKLGFGRTVIREALQRLAFAGIVSLQRNRGAFVADPSLVEVQH
                     AYAARRLIEGEVVADVCRHCTAHDIRALRKHLDQQIAAQASGDRGRFVQLLTEFHSLL
                     ASLGENRLLESFVQQLAAKTSLAVLLYDGEPPVCGLEEHAALIDLLAAGDVEAARDLM
                     SRHLRGNQERLPPGKPA"
     gene            7296..8231
                     /locus_tag="VKU03_02645"
     CDS             7296..8231
                     /locus_tag="VKU03_02645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLJ70188.1"
                     /translation="MTNLSNRDFKGYGGKPPDIRWPGGARVAVSFVVNLEEGAELSIS
                     DGDERNEGAYEAVEEIRDLPDPCQDSHFEYGIRAGWPRVAAALAEHKAVATLNACGRA
                     VDRSPWLARQAVEQGHEVSCHGWRWERHAGMSEAEERRAIAQTVTAIRKATGVAPVGW
                     HTRSAPSVNTRRLLLEHGGFIYDSDAYCDDLPFFVDVAGQRRLVLPYAFDTNDMNFFQ
                     THRFVTGDDFASYVIAAFDRLWAEGASEPRMLSIGLHLRMIGRPGRIGGLETILRHMA
                     QRGDAWIARRDSIARHWLAHSSVETANKECEASYS"
     gene            8210..8929
                     /locus_tag="VKU03_02650"
     CDS             8210..8929
                     /locus_tag="VKU03_02650"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HLJ70189.1"
                     /translation="MRSVLLINPNTNRAMTQQMAKAAARCLPEGVALQALTADFGFPV
                     IATRVSFAIAAHAVLDTYARSPDVHDAVVIGCFGDPGLEALREIASEPVFALAESSIL
                     AADARGEPFAILTLGEAWIDLLTERAKLAAVKAPFIGVFAGEGTGLDAERQDMALARR
                     LERQAREAISAGARTLILGGGALAGMASRLNIDAVLIDCMEATMRLAIAPARPALRRR
                     QPTQPSGLTEHLTKRLIDAAA"
     gene            complement(8944..10446)
                     /gene="glpK"
                     /locus_tag="VKU03_02655"
     CDS             complement(8944..10446)
                     /gene="glpK"
                     /locus_tag="VKU03_02655"
                     /EC_number="2.7.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004370 - glycerol kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol kinase GlpK"
                     /protein_id="HLJ70190.1"
                     /translation="MSAPLLLAIDQGTTSTRSIVFTGDLSIAAVAQEEFPQHYPAPGW
                     VEHDPEDIWRTTVSTIRAALAKAGAAAPQIAAIGVANQRETTLLWRRATGKPIGRAIV
                     WQDRRTHEACARMRAAGQDRLIAEKTGLILDPYFSATKIAWLLDSIPGARAEAERGAL
                     AFGTVDAFLLWRLTGGKVHATDATNAARTALFDIRRGVWDEELCRLFNVPVSILPKVM
                     DCAADFGTADASILGAPVAIRGVAGDQHAAVIGQACFAPGMAKITYGTGCFALLNTGA
                     APVASQKRLLTTIAYQLEGRRTYALEGAIFVAGAAVQWLRDGLGLIRSASDSGVLAAQ
                     ADDAQSVYLVPAFVGLGAPYWDAEARGALFGLTRGTGPKEIARAALESVGYQTRDLVE
                     AMRADWTAPGETVLRVDGGMTASDWTMQFVADILGAPVDRPKIMETTALGAAYLAGLA
                     QGLLPPPDRFAETWALDRRFAPALAREARERKFAGWRDAVRRTLSFDFPA"
     gene            complement(10588..11754)
                     /locus_tag="VKU03_02660"
     CDS             complement(10588..11754)
                     /locus_tag="VKU03_02660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016556294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ70191.1"
                     /translation="MTKTLNIAMIGGGFMGKAHAMAYATMPMFFWPAPAKPRLKAIVD
                     VTAELAETGRQRYGFDEASTDWRSVVGRSDIDVVDIVTPNDSHAEIAIAAAKAGKHII
                     CEKPLARTAAEAKAMLDAVEKAGVVHMVAFNYRRTPAVTLAKKYIDEGRIGRILSFRG
                     TYLQDWSADPNGPLSWRFQKKIAGSGAVGDIGTHVIDLARYLVGEISAVNAMVKTHIA
                     SRPLQSGGFDQLAASDKSGKGPRGAVDVDDEMITLVKFAGGAFGSIEATRNAYGRNNF
                     ITFEIHGDQGSIAFNYERLDEIQVMFASDPADARGFRTVYTGPSHPYGGALWPIPALG
                     TGYGETKIIECHDFLNAVVRGETPSPNFRDGFRISQIADAILESGESERWVVVD"
     gene            complement(11818..12123)
                     /locus_tag="VKU03_02665"
     CDS             complement(11818..12123)
                     /locus_tag="VKU03_02665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012555754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HLJ70192.1"
                     /translation="MKPCTIIGTVVAKDETRDELRAILSAQVGPTRAEPGCISYDFHV
                     DAENPNRFMFYENWRSRDDLDAHLQAPHLKPLFGRLDELVAEPVDIHFYEMLSPWEH"
     gene            complement(12144..13115)
                     /locus_tag="VKU03_02670"
     CDS             complement(12144..13115)
                     /locus_tag="VKU03_02670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70193.1"
                     /translation="MRAASAAPPAPRRRSRFADWRQYVAYLGFALIFVFFSVTLFDKG
                     FLDPNNLLNILRQTAMISIMSVGMTYVLSAGEIDLSIGMVAGLVSLVAAVAIDGYGPA
                     AGVAAGLAVGALVGFVNGVLTTRIGIPSFLTTLAMMGIARGSAMWMTQTKSVPILDDD
                     FIWLFGGGNLGPVPILVLWTLILAAIGHVVLRRTTFGRRVLATGGSEIAARYSGIDTR
                     SVKMRVLIISSLTAAIAGMLYAGRLHSGRFQLGEGDELSVIAAAVLGGTSLFGGSGTV
                     VGSIIGSLMIGVINNGLLLMGLEYSQQLIARGLIILIAVAISQSRKR"
     gene            complement(13102..14661)
                     /locus_tag="VKU03_02675"
     CDS             complement(13102..14661)
                     /locus_tag="VKU03_02675"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ70194.1"
                     /translation="MSSNARPAVRLAGVSKSFGGVRALDNVDFEVSSGEIHALLGENG
                     AGKSTILKILNGVHAPSAGRIEIDGAPLSQHTPEAARRAGVGMIFQEMSLTPTLTVAQ
                     NIFLNQELKGRLGFIDDEEAARRSRELFDSLGVEVDPLARVGDLSAGQRQLTEIVKAI
                     SRDVRVLILDEPTTALSGGEVEKLFAILRRLKAKGVAIIYVSHRMDEIARIADRATIL
                     RDGRRVITAPMSELTLDTIIEHIVGRRSRGFSDVARKAARRGAPLLEVKGLSGLQKPE
                     DVDLTLYAGEVVGVAGLLGSGRSALARVLFGVDRKRSGEIRIQGVPVEIGSPRDAIAR
                     GLVLVPEDRLRQGLILEHSVEANASLAILDRLASWFFVSRAKAADAVDRQIAALRIRT
                     ASRDAAARTLSGGNQQKVVVAKWLSAEPDIFVLDEPTAGVDIGSKAEIVALIRGLAER
                     GKAVLAISSELSELLTVSDRILVMADGRIVSEHARAALDDDNPNDDVGEQLHHAERRL
                     SSIVQRAYARR"
     gene            complement(14767..15885)
                     /locus_tag="VKU03_02680"
     CDS             complement(14767..15885)
                     /locus_tag="VKU03_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ70195.1"
                     /translation="MMRTKFLTACAAIATIGAFGAPAYATDPDAALAQMKNQVFSLGP
                     NGEKPTAASTVTLTADELAKVKAKHAKAALVFHYGGNDWSNAQEAALKAQFAAEGIDV
                     IATTDAGFKPEKQVADIETVMAQKPDIIVSIPVDAVATAAAYKAAADKGVKLVFMDNV
                     PKGFTAGKEYVSSVSADNYGNGVAAAHLMAKALGGKGEIGLVFHAADFFVTRQRYDGF
                     KKTILQDYPDIKIVGEQGIGGPDFSGDAEKAASALLTEHPRLNGIWAVWDVPAEGVMA
                     AARAAGRDDLTVVCEDLGENVAIAMAKKEFIKGIASQRPYDQGVTEAKLAAYALIGKP
                     APAYVALPAAPITRDTLLAAWQEIYHQPAPEKVVKSMK"
     gene            complement(15909..16742)
                     /locus_tag="VKU03_02685"
     CDS             complement(15909..16742)
                     /locus_tag="VKU03_02685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLJ70196.1"
                     /translation="MRARLAYHANCWGPLGGDAVGVTSIAQLAYRTFGDMGRAIAEIG
                     AVGYEGVELFDGNLLDYDGRRSDLGGALDRAGLKLVAVYCGANFIFPDILAEELARIT
                     RAAEAAAAFGAEHLVVGGGAKRAGGVKADDHQRLGEALEKVVAIAKARGLKAHYHPHL
                     TTIVEGPAEVDKIFALTSIDFCPDTAHLAAAGGDVAEMIRKHRRRISYVHLKGWTREP
                     FAFTPLDRGDLDIAAIVDALAEVDYRGWITAELDSWPDPRDGAARSLGFLRGALAKSP
                     G"
     gene            17081..>18367
                     /locus_tag="VKU03_02690"
     CDS             17081..>18367
                     /locus_tag="VKU03_02690"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLJ70197.1"
                     /translation="MLNLIRRGEATTRLDLERKSELGRAALVDRLGKLQALGLAAEGD
                     LGASDGGRAPRHMRFLAETGAILIASIERASMSVALADLSGRLIVEHHEAVDLADGPE
                     AILDRVTNLFIWLMDERGGKERVWAMGLALPEPVLADTNDGDAFGIVSLDALRSWRGF
                     DFESELSLRFKAPCWARGGAQMMTFGEFKHGACQGAGDLLFVKLGHSIGAGMVSAGRL
                     HRGAQGLAGMIGHAPTGESGGAPACHCGAKGCLEALASGEAIAREARLAAQDGRSRYL
                     ADMAGLNGEVTATDVGHGAQLGDAFCADLLSRCGRLVGESLASLVNLLNPAFVVLGGS
                     VAESADVLLAAFREAIYRQSHPLVTRDLRIVRSQMGETAALLGAAHLVLDEVFSPAYL
                     SDWIAHGSPRRRPAFLEFLETERGKRRASAPAPRPPR"
BASE COUNT         3069 a         6087 c         5998 g         3213 t
ORIGIN      
        1 cgaatacggg cacttcttcg agcacaatcc gatcgagatc gtcaacgtgc gcgtcaccgg
       61 cgtcggcgca atgcccaaaa tccagaagct gcgcgcaccg gcgggcggct ccgtcgccgc
      121 cgcgaccgtc cgggccggcc agtgcatgtt ccgcgtcgat ggcgagttca ggccgttcga
      181 gacgacgttc taccgccgcc atctcctgcc ggtcggcgag acgatctccg gtccggcggt
      241 tatcctgcag aaagacagca cgaccgtgat accgccgcgc tggtcgtcgc ttaccgacgc
      301 cgccggcaac ctcatcctta aattcgacgg agacgcctga tgcaaccgtt cgaggggaca
      361 aacacgcccg cccggctcgc cacccgggtc gatccgatca ccggagcggt gatccaaggc
      421 gccttggaga acatcgccat cgagatgggt cacaagctga tgcggatgag ctattcgagc
      481 atcatccgcg aatcggagga tttcggcgcg gcgctcaccg acgccgaagg caatcagctc
      541 tgcgaatgca agatgagcac gccgcttcag tccgggccga tccccggcta tgtgcgcggc
      601 gtgaaaaaac gcttcgcggc gcgcggcgac gtcttccgcc ccggcgacgt gatcatgcac
      661 aacgacccct atggcggcgc ttcgcatgga ccggacgtcg ccttctgcgt gccggtcttc
      721 gacggcgaaa cgctgctcgg cttctccgtc acgacggccc accacctcga catcggctct
      781 cacacgccgg gcagttgcgg catcgtcgac gcggtcgacg cctacgccga gggcttgcaa
      841 ttcaaggcga tcaaggtcgt cgatcagggc aagcgcaacg acatggtctg gcacatgctc
      901 cgcgacaaca tccgcatttc cgatctcgtc gtcggcgaca tggaggcgca gatcgccgca
      961 gcccagatcg gcgcgcagcg ctttctcgat ctcgtccggc attacgggcg cgacacggtg
     1021 aacggcgcca gcgccgatct catggactat tccgaacgcc tgatgcgcgc cgcgatcggc
     1081 gccattccgg acggcgtcta tagcgcctca acctatattg acggctttct cgacgacccg
     1141 gagccgtcgc gccgcgacct gaaaatcgtc gtcacgatca ccgtcaaagg cgacgagatg
     1201 ctggtcgatc tcgccggcac ggcgccgcag gtgccggatc gcccgatcaa catgccgttc
     1261 gaggggacgg tcgattgcgc gatctggctg accttgcgct cgatcctgct cgacagcgtc
     1321 gtctatggcc cgattccgca aaatagcggc ctgacgcggc cgatcagaat cgtggcgccg
     1381 aaaggctgcc tcgccaatcc ggtcttcccg gccccggtga tcgcgcgctt ctgtcccggc
     1441 aatgcgctcg ccgacgcggt gatgaaagcg ctggcgccgg ccgtgcccgg ccagatcagc
     1501 gccggcatcg gcaacctgac cgtcatcgcc ttcagcggcg tggcgaacga ccagcaatgg
     1561 gtgcatatgg aaatcctcga aggcagctat ggcggccggc gcggcatgga cggcatggat
     1621 acggtcgaca cgctctacgc caatacccgc aacaacccga tcgaggacat cgagtcgcat
     1681 ctgccgttgc gggttgaacg ctacgaactg cgcgaggacg tggcggcgcc gggcgagtgg
     1741 cgcggcggcg tcggctcgat ccgggagttt cgctatctga cggacggcgg cttttcggtc
     1801 gagggcgacg gacacaaatt caagccatgg ggctttgacg gcggggcgga cggcgcgccg
     1861 agcgctcttg tccaggcaag ttcgtccggc gaacgcatcg aactgccgtc gaaagtgccc
     1921 tatcgcaagg cgaaggcggg cgaccggctg atctccctcg gcccaagcgg cggcggttac
     1981 ggaaatccgc tggcgcgggc gccggaggcc gttctcgaga atgtcctgga cgggatcatt
     2041 tccgcggacc ttgcccgccg acaatacggc gtggcgatcg cgaacggcga aatcgacgcc
     2101 gccgccaccg aggcgctgcg cgcgaaaatg cgactccgat agacgcaagg aggccggcct
     2161 tcgcgccggc taggcgctga gaaactcgtt ccaccggcgc gccagataat tgtggtcgtc
     2221 catgatcgtg ttccagacgc gcacgcgcga catccggtcg atataggagc cgccgccgcg
     2281 tatctcgccg gcccgcacgc aggtctcgcc gaacgggtcg ggcaggtcga cgcgcgccgg
     2341 cttgccttcg taccaatagt cccactccgc ctcggacaga tggcggcgcg tgcgctcggg
     2401 cgccgacata tagtagccct gccgcgccag gatcgcgccg gccgggccct ccatccacca
     2461 gttcagatac gcgtaagccg cctccagcgt ttcgccggaa gcggccgccg acaggcagag
     2521 atcggagtgc cagccgcgcg agccttccgc cggcatcgcg ctcatcacat cgaggccgcg
     2581 gcggcgaagc ttgatgaccg ccggcgagaa aatgctttga acgacgacgc cgccgcgctc
     2641 catcagcctg accgcgtcct cgtacgtttc ccagaggccg cgaaagtggc cgttgcgttt
     2701 cttgtccttc aaaatcgcga tgatcgcgtc gatctcctcg atggacaagt tggcgatatc
     2761 ggcgaatttc aggatcccgg cgccctccat cgccagggcg gcctcgatca tgccgagcgt
     2821 cggatcgcgc agcatggcga ccttgccgcg ccaccggtcg tcgatcagcc aagcccagct
     2881 gtccctctcg ccagggcgaa gctgctcgcg caggtttttc agatagccaa gagaatccgt
     2941 cccgtggatc gtcggcagca tgctggcgag gcgcgtcgga gaagaggcga gccggccgtc
     3001 gtcctgaacg tagagctggc ggcaaagatc gtcgcagatc gtcgcgccgt ctggggacaa
     3061 cgacgccaag gaggcgatct cgctccaggc cgggatacgc tcgatcgcaa tcggctggat
     3121 cgagcgcgcc gtccacatca gatcgaccgt gtgccattga tgatagacgt cgaaactgtc
     3181 cggccgcgtc gccgcctgcc ggatttccgc cgccccttcg agcagttcga actcgatatc
     3241 gaagccaagg tccttcgccg cttggcggcg gaccggctcc aacagaatct cgcgcctgcc
     3301 gagcacccgc agcgccggac gcgttttgcg cggccggcgc ttgcggtcgg cgcgcaacgc
     3361 gcgcgacttg tgagacgcgg aacgtccgtc gacgatcggc gtcagatagg gcgccacgtc
     3421 ctgcgccgtg agcaccgaca ggactttcag ccgcgcccgg gtccgctcca tggcgcgccc
     3481 gatatgaaag gagagcgcgg cggcggcttt ctccacctcg ccggccagca gatggtcgag
     3541 aacctgcgca tgctccttca gcgccgccat ggcttcgctc aggccgaagt gctttgcgaa
     3601 cagccgattg atgacgtccg gcatccggct ctgttggatc gtcgcggtga gccggcgatt
     3661 cgagacggag gcgaaacagg cctcgtgaag gtcacgctcg atctcctcga cctcgcgcgg
     3721 cgtcaatgaa tgcgccgcct gcagcgcctg ctcgatacgc gcccgcatcg tctcgacggt
     3781 ccgccgatcg agagacttcg ccgcgaggcg cagagccgcc ggctcgagca attgccggat
     3841 ctcgaaatgc tccttgacgt cgcgcgcggt gagcgggccc gccgtccagt gcgaccagcg
     3901 atccttctcg atcaggccgt catggttgag gcgggccaag acgtcgcgca cgaccgtgcg
     3961 gctcacgtcg aagtgagcgc acattgccgg ctcagaaatc ttgtaggcgc cgaacggcat
     4021 gcaggcgacg acgctctccg ccgcctcgcc ataaattttc tgccaggcgg cgcgctgggc
     4081 gtagtcctgg atcacgccgg aaacatcgag gccgagcgcc agaaggttca cgcggagcgg
     4141 cgccgcgtct tcccccgccc cgccgacgag atagccgcgc ccgcggaacc ggcggatgag
     4201 gccttcatcg gccagcaatt cgagagcccg cttcaccggc cagcggctga ccccgagcaa
     4261 tgtggcgatc ggcccttcga gcaggacgat tccaggccga agcttgccga cctcgatgtt
     4321 gcgcgcaatt tcgtcccgca ccagctcgta gtaaggccga ggctcatgca gaacggagga
     4381 gctctccgtc gccgggacta ggctgggagc cggatcgtcc aatttgacgt gattcataat
     4441 cgcgacggaa cgggcttcta aatggtcttc gtggaagcgt tttgccttgc ccaaacaggc
     4501 ggttgtttgg gaactatacc ataaatccgg tcattccatt tcgagcgcgc atctttgagc
     4561 tgctcgcgct ggactgtgcg gcctctctcg tacctctgaa gcgccaaaac gcaaagccgc
     4621 tgcggcgaca aatcggcctg atcccgatcg ccggaaacgg gccagtctat atagatgaat
     4681 tcagacgtcc gacgcgatcc ttgctatggg atggcgtcat tttcagtttg acgacgatct
     4741 catattttgc atacaattat tgaaactcgc cgataatttt gtcgagtttt gggagggtcc
     4801 tgcaatgctc agcaatcccg cggccgtttc gcccggcgaa gcgcgcaatc cggcgtcgcc
     4861 ggcgatcccg gacgtttcgc ccctgaccaa aggccaggtc gcctataccg cggtcgtcgc
     4921 gctgctcgcc tggacctttg cggtctacga cctgatcacg ttcggcaatc tgctgccgaa
     4981 aatccaacaa gagttcggct ggtccaatgc gaccgcgtcg tacgtcgcga ccctggtggg
     5041 actcgcctcg ctcgttgtcg ccctctgcgt cgggccgatc atcgatgggc tcggccggcg
     5101 gttcgcgctg gtcctgacga ccggcggcgc ggcggtgagt tcaggccttg cggctctggc
     5161 cttcggcccg gtgtcgctgg tcgtctttcg cgccctgtcc ggcttcggca tgtcggaaca
     5221 agccgtcaat tccgcctatc tgaacgaagt cttcgccgcc cgcgccaaag gctttctcta
     5281 cgggatcgtg caagcgggct ggccgctcgg cgtgatgctc tcatcggggc tcgccgcctt
     5341 gctcctgccg gagatcggct ggcgaggcgt tttcctggtc gccgcctttc cgcttctggt
     5401 gatgctcgtc tgccggttcg gactgagaga atcgccgtat ttcctcaagg tacagcacct
     5461 gcgtcgcttg cgggaccgcg gcgacgccgc cggcgccgag gagctcgccc ggcgctggag
     5521 cctcgatatg acaggcgacc ggtggaacac ttacgccgag ctgttccgcc cggccttgcg
     5581 ccggcagtcg atcgcgctcg gcgcgatgtt cttcttcaag atcatcgccg actcgcaatt
     5641 gacggtcctc gccacctcgg ttctgggcca agccaagggc atcgacctga ccagcgcgct
     5701 ctggaccatc tttttcggca acggcgtcgc ggtgctcggc tatctgttcc tcggctggac
     5761 gggcgacagg atcggccggc gcgaaaccgt gatcatcgcg caaaccctgg cggcggtctg
     5821 cacgttcgcg ctgctgttcc tcgcccatgg ccttgccggc gtgatcgtgt gctacgccct
     5881 cgttctattc ttcgcgcagg gcgccgccgc gccgttcttc gcctatgtcg gcgaatcctt
     5941 cccgacccgc gtccgcggct cgggcgccgc ctacatcaat gtcgccggcc cgatcggcgg
     6001 cattttcggt ccgctggtct acggcgcgct gcaacaggcc ggggcgagcc cgaccgccgc
     6061 cgcagcatct ggcggcgtgg cggcgctcgt cgcggcggct tgcatgctgg ccgcccggtc
     6121 gatccgtccg ggtcaggagc tgtccgatat ctcgcactga cgccgagcgg tcgctcaagc
     6181 cggcttgccg ggaggcaagc gctcttgatt gcctctcaaa tgccggctca tcaggtccct
     6241 cgccgcctcg acgtctccgg ctgcgaggag atcgatcagg gcggcgtgct cttcgagccc
     6301 gcaaaccggc ggttcgccgt cataaaggag cacggccagc gaggtcttcg ccgcgagctg
     6361 ctggacaaag ctctcaagga ggcgattttc tcccaaagag gcaagcaacg aatgaaactc
     6421 ggtgagcaat tgcacgaacc ggccgcgatc gcccgaagcc tgggcggcga tctgctggtc
     6481 gagatgcttg cggagcgcgc ggatatcgtg cgcggtgcag tggcgacaga catcggcgac
     6541 cacctcccct tcgatcaggc ggcgcgccgc ataggcgtgc tgcacttcga cgagcgaggg
     6601 gtccgcgaca aaagcgccgc gattgcgctg caagctgacg atgcctgcaa aagccaggcg
     6661 ctgcagcgcc tctctgatca cggttcgccc gaagccgagc ttcgcgccca gcgcggcttc
     6721 gctgagcttc tgtccgggac gaaaccgctc cttgacgatc agcgacgcaa tctcatgggc
     6781 gaccctgtcg tcggaagcaa ggccatggaa caggccgcca tggccgaact ggcggatcgg
     6841 cgcaggatcg ggcgcggccg tttcgcgcgc cgcttcgctg cgcttcgtct tcatcggctc
     6901 gccggcgcgc ttgagaacct tgaggatcgt ctccggcgac aggttcaggc cgtgccgccg
     6961 ccggagctcc gacctcagcc ggtgaacgcc gagattctcg ctcttgcgca aagcaagaat
     7021 cagcgcctcc tcgcgcgcga agaccttttg aagcggagac gaaaccggcc tgcggctgga
     7081 atcggtcaag ccggctggtc caaattcctt gtatttccgc caccatttgc gaagcgtcgg
     7141 tcgcgaaatg ccgaacttgg cgcaaacgaa gccagcgcgc cccgtcgcct cataggcctc
     7201 tatccagcgt aggcgctcgt ctccggacac gatagactct cgatatgatc tacacacact
     7261 gattcaacca accggtcccg tcaagaggaa attgaatgac caatttgtcc aatcgcgact
     7321 tcaagggcta tggcggcaag ccgccagaca ttcggtggcc gggcggcgcg cgcgtcgcgg
     7381 tctcgttcgt cgtcaatttg gaagaaggcg ctgaactctc gatttctgac ggcgatgaga
     7441 gaaacgaagg cgcctatgaa gcggtcgaag aaatccgcga tcttcccgat ccgtgccagg
     7501 attcgcattt tgagtatggg atccgcgctg gctggccgcg cgtcgccgcg gcgctggcgg
     7561 agcacaaggc cgtagcgacg ttgaacgcct gcggccgcgc ggtcgaccgt tcgccatggc
     7621 tggcgcgcca ggcggtcgag caaggacacg aggtgtcctg tcacggctgg cggtgggagc
     7681 gccatgccgg gatgagcgaa gctgaggagc gccgcgccat cgcccagacc gtgacggcca
     7741 tccgcaaggc gacgggcgtg gcgccggtcg gctggcatac gcgctcggcg ccgtcggtca
     7801 acacgagaag gcttctcttg gagcatggcg gcttcatcta tgacagcgac gcttactgcg
     7861 acgatctacc gttcttcgtc gacgtcgccg gccagcgccg gctcgtactg ccctatgcgt
     7921 tcgacaccaa cgacatgaat ttcttccaga cgcatcgatt cgttactggc gacgatttcg
     7981 ccagctacgt gatcgccgcg ttcgaccggc tctgggcgga aggagccagc gagccgcgca
     8041 tgctctccat cggcttgcat ttgcgaatga tcggccgccc gggccgaatc ggcggcttgg
     8101 agacgatcct gcgccatatg gcgcagcgcg gcgacgcctg gattgcgcgg cgcgattcca
     8161 tcgcccgcca ttggctggcc cattcctcgg tcgagacggc gaataaagaa tgcgaagcgt
     8221 cctactcata aatccgaaca ccaaccgcgc gatgacccag cagatggcga aagcggcggc
     8281 gcgttgcctg ccggaaggcg tggcgcttca ggcgctgacc gccgatttcg gctttccggt
     8341 catcgcgacc cgcgtttcgt tcgcgatcgc cgcgcatgct gttctcgata cgtacgcgcg
     8401 gagcccggat gttcacgatg cggtcgtcat cggctgtttc ggcgatccag gcctcgaagc
     8461 gctgcgcgaa atcgcgtccg agccggtatt cgccttggcg gaatcgtcga tcctcgcggc
     8521 ggacgcgcga ggcgagccgt tcgccattct gacgctgggc gaggcgtgga tcgacctgtt
     8581 gaccgagcgg gcgaaactcg ccgccgtcaa agcgccgttt atcggcgtct ttgccggcga
     8641 agggacaggt ctcgacgccg agcgtcagga catggcgctc gcccggcgcc tcgaacgcca
     8701 ggcgcgggag gcaatttccg ccggcgcccg cacgctcatt cttggcggcg gggcgctcgc
     8761 cggaatggcg agccggctca atatcgacgc cgttctgatc gattgcatgg aggcgacgat
     8821 gcgcttggcg atcgcgccgg cccgccctgc gctgcggcgc cgccagccga cgcagccctc
     8881 cgggctgacc gagcatctca caaagcgctt gatcgatgcg gcggcgtaac gcggcgacgc
     8941 aaatcaggca ggaaaatcga acgacagggt acgccggacc gcgtcgcgcc agccggcgaa
     9001 ttttcgctcc cgcgcctcgc gcgccagcgc cggcgcaaaa cggcggtcga gcgcccaggt
     9061 ctccgcgaac cggtccggcg gcggcaggag cccctgcgca agcccggcca aataggcggc
     9121 gcccagcgcg gtcgtctcca tgattttggg acgatcgact ggcgcgccga gaatgtcggc
     9181 gacgaattgc atcgtccagt ccgaggcggt catgccgccg tcgacgcgca gaaccgtctc
     9241 gcccggcgcc gtccaatcgg cgcgcatcgc ctccacgagg tcgcgagtct ggtacccgac
     9301 gctctccaag gcggcgcggg cgatctcttt cggccccgtg ccgcgggtca ggccgaacaa
     9361 ggcgccgcgc gcctcggcgt cccagtacgg cgcgccgagg ccgacgaaag ccggaacgag
     9421 atagacgctc tgcgcgtcgt cggcttgcgc ggccagcacg cccgaatccg atgcggatcg
     9481 gatcagtccg aggccgtcgc gcagccattg gaccgccgcg ccggcgacaa aaatggcgcc
     9541 ttcgagcgcg taggtccgcc ggccctcaag ctgataggcg atcgtggtca agagccgctt
     9601 ttgcgacgcg accggcgcgg cgccggtgtt gagaagcgcg aaacagcccg tgccgtaggt
     9661 gatcttcgcc atgccgggcg caaagcaagc ctgaccgatg acggccgcgt gctgatcgcc
     9721 ggcgacgccg cgtatggcga cgggcgcgcc caaaatcgaa gcgtcggccg tcccgaaatc
     9781 ggcggcgcaa tccatgacct tgggcaggat cgaaaccggg acgttgaaca ggcggcaaag
     9841 ctcctcgtcc caaacgcccc ggcggatatc gaacaaggcc gtccgcgcgg cgttggtcgc
     9901 atccgtcgca tggaccttgc cgccggtcag gcgccagagc agaaaggcgt cgacggtccc
     9961 gaaagccagc gcgccccgtt cggcctcggc gcgcgcgccc ggaatgctgt cgaggagcca
    10021 ggcgattttc gtggccgaaa aataaggatc gaggatcagc ccggtcttct cggcgatgag
    10081 ccgatcttgc cctgcggctc tcatgcgcgc gcaggcttcg tgcgtgcggc ggtcctgcca
    10141 gacaatggcc ctgccgatcg gcttgcccgt cgcccgccgc cagaggagcg tcgtctcgcg
    10201 ctggttggcg acgccgatcg cggcgatttg cggcgccgcc gcgccggctt tggccaaagc
    10261 ggcgcggatg gtcgacacgg tcgtgcgcca aatgtcctcg ggatcgtgct cgacccatcc
    10321 cggcgccgga tagtgctgcg gaaactcctc ctgcgcgacg gccgcgatcg acagatcgcc
    10381 ggtgaagaca atcgaccgcg tcgacgtcgt gccctggtcg atggcgagaa gcagcggcgc
    10441 gctcataggc ggcggcccaa accggctatg ccaggatttg cttccctctc ttcacgcggc
    10501 acgaatgcgc ctctcgtctg aaagacgaga gggcttacca cctccgccgc gcacggcaaa
    10561 atgcgccgcg ccgagggcgc cgaagcgcta gtcgacaacg acccatcgct cgctttcgcc
    10621 cgattccagg attgcgtcgg cgatttgcga aatcctgaat ccgtcgcgga aatttggcga
    10681 gggcgtctcg cccctgacca ccgcgttgag aaagtcgtgg cactcgatga tcttggtctc
    10741 gccatagcct gtgccgagcg ccgggatcgg ccacagcgcg ccgccatagg ggtgcgaggg
    10801 cccggtatag acggtgcgga atccgcgcgc gtcggccgga tcgctggcga acatcacctg
    10861 gatctcgtcg aggcgctcgt agttgaacgc gatcgagcct tgatcgccgt gaatttcgaa
    10921 ggtgatgaaa ttgttgcggc cataggcgtt gcgcgtcgcc tcgatcgagc cgaaggcgcc
    10981 gccggcgaac ttcaccagcg taatcatctc gtcgtcgaca tcgaccgcgc cgcgcgggcc
    11041 cttgccgctt ttgtccgaag ccgcgagttg atcaaagccg ccgctttgca gcggccggct
    11101 ggcgatatgc gtcttgacca tggcgttgac cgccgagatt tcgccgacga gatagcgggc
    11161 gagatcgatg acgtgggtgc cgatgtcgcc gaccgcgccc gagccggcga tcttcttttg
    11221 aaagcgccaa gagagcggcc cgttcggatc ggccgaccag tcctgcagat aggttccgcg
    11281 gaacgaaagg attctaccga tgcggccttc gtcgatgtac ttcttggcca acgttacggc
    11341 gggggtacgc cggtagttga aggcgaccat gtgaacgacg ccggcctttt cgacggcgtc
    11401 gagcatcgcc ttggcctcgg ccgcggttcg cgccagcggc ttttcgcaga tgatgtgctt
    11461 gccggccttg gccgcggcga tggcgatttc ggcgtggctg tcattcggcg taacgatgtc
    11521 cacgacgtcg atatcgctcc gccccacgac gctgcgccaa tcggtcgagg cttcgtcgaa
    11581 gccgtagcgc tgacggcctg tttcggcgag ttcggccgtg acgtcgacga tcgccttgag
    11641 gcgcggcttt gccggcgccg gccagaagaa catcggcatg gtcgcatagg ccatggcgtg
    11701 cgccttgccc atgaagccgc cgccgatcat cgctatgttg agagtcttgg tcattctcgc
    11761 aaaggtccct gttgtattga atcgtccggc tccgccaatc gaggcggcgc attcgcctca
    11821 atgctcccac gggctcagca tttcgtagaa atgaatgtcc accggttcgg cgaccagttc
    11881 gtcgagtcgc ccgaaaagcg gtttgagatg aggcgcctgc agatgcgcgt cgagatcgtc
    11941 ccggctccgc cagttctcgt agaacatgaa gcggttcggg ttttcggcgt cgacatggaa
    12001 gtcgtagctt atgcagccgg gctctgcgcg ggtgggaccg acttgagcgc tgagaatcgc
    12061 cctgagctcg tcgcgcgtct cgtctttcgc caccaccgtt ccgatgatcg tgcaaggctt
    12121 catgttgaag gcgctccccg ctttcatcgc ttgcgtgact ggctgatcgc caccgcaatg
    12181 aggatgatga ggccgcgggc gatgagctgc tggctgtatt cgagccccat gagcaaaagg
    12241 ccgttgttga tcacgccgat catcagggag ccgatgatcg agccgaccac cgttcccgag
    12301 ccgccgaaca agctggtgcc gccgagcacc gctgcggcga tcaccgaaag ctcgtcgccc
    12361 tcgccgagct ggaatcgtcc cgaatgcagc cggccggcgt aaagcatgcc ggcaatcgcg
    12421 gccgtcaggc ttgaaatgat caggacgcgc atcttgaccg agcgcgtatc gatcccgcta
    12481 tagcgcgccg caatctcgct gccgccggtc gccagcacgc ggcggccgaa cgtcgtccgg
    12541 cgcaggacga catggccgat agcggcgagg atcagcgtcc acaaaacgag aataggaacg
    12601 gggccgagat tgccgccgcc gaaaagccaa atgaaatcgt cgtcgaggat cgggaccgat
    12661 ttggtctggg tcatccacat cgccgagccg cgcgcgatgc ccatcatggc gagcgtcgtc
    12721 aggaaggatg ggatgccgat gcgggtcgtc aacacgccgt tgacgaagcc gacaagcgcg
    12781 ccgacggcga ggcccgccgc gacgcccgcg gccggtccgt acccgtctat ggccacggcg
    12841 gcgacaaggg aaaccagccc cgcgaccata ccgatcgaaa gatcgatttc gccggccgag
    12901 aggacatagg tcatgccgac cgacatgatc gagatcatcg cggtctggcg caggatgttc
    12961 agcagattgt tcgggtcgag aaagcctttg tcgaaaagcg tgaccgagaa gaaaacgaaa
    13021 atcagcgcga aaccgagata ggcgacatat tggcgccagt cggcgaaccg cgagcgtcgg
    13081 cgcggcgccg gcggcgcggc gctagcggcg cgcataggcc cgctgaacga tcgaagagag
    13141 tcggcgttcg gcatggtgca attgctcccc gacatcgtcg ttgggattgt cgtcgtcgag
    13201 cgccgcgcgc gcatgttcag agacgatgcg cccgtcggcc atgacgagaa tgcggtccga
    13261 gacggtcagc agctccgaga gttcggagga gatcgccagc accgccttgc ctcgctcggc
    13321 gagcccgcgg attagcgcga cgatttccgc cttcgagccg atgtcgacgc cggccgtcgg
    13381 ctcgtcgagc acgaaaatat cgggctcggc gctcagccat ttggcgacga cgaccttctg
    13441 ctggttgccg cccgagagcg tgcgcgcagc cgcgtctctg gaggcggtcc tgatccgcaa
    13501 ggcggcgatc tgccggtcga ccgcgtccgc cgccttggcg cgcgagacga agaaccagga
    13561 agcgagacgg tccagaatgg cgaggctcgc attggcttcg acgctgtgtt cgaggatgag
    13621 accctggcgc agccggtctt ccggaacaag gaccagtccc cgcgcgatgg cgtcgcgcgg
    13681 gctgccgatc tcgaccggaa cgccctggat gcggatttct ccggatcgct tgcgatcgac
    13741 gccgaacaag acccgcgcca aggcgctgcg cccgctgccg agcagcccgg cgacgccgac
    13801 gacctccccg gcataaagcg tgagatcgac gtcttccggc ttctgcaggc cgctgagccc
    13861 tttgacttcg agaagcggcg cgccgcgacg ggcggctttc cgggccacgt ccgagaagcc
    13921 gcgcgatctt cggccgacga tatgttcgat gatcgtgtcg agcgtcagct cgctcatcgg
    13981 cgcggtgatc acccgccggc cgtcgcgcag gatcgtcgcg cggtcggcga tgcgggcgat
    14041 ctcgtccatc cgatgcgaga catagatgat ggcgacgccc ttcgccttga gccgccgcag
    14101 aatggcgaaa agcttctcga cctccccgcc tgacagcgcg gtggtcggct cgtcgagaat
    14161 gaggacgcgc acatcgcggg aaatcgcctt gacgatttcg gtcagctgcc tctggccggc
    14221 ggaaaggtcg ccgacgcgcg ccaacgggtc gacctccacg ccgagactgt cgaacaattc
    14281 gcgcgagcgg cgcgccgcct cttcgtcgtc gatgaagccg agccgccctt tcagctcttg
    14341 gttgagaaag atgttttgcg cgacggtgag ggtcggcgtc aggctcattt cctggaagat
    14401 catgccgacg cctgcccgcc gcgccgcctc gggcgtgtgc tggctgagcg gcgcgccgtc
    14461 gatttcgatg cggccggcgc tcggcgcgtg cacgccattg aggattttga gaatggtcga
    14521 cttgccggcg ccgttttcgc cgagcagggc gtgaatctct cccgacgaga cttcgaaatc
    14581 gacattgtcc agcgcgcgga cgccgccgaa gctcttcgag acgccggcta aacgcaccgc
    14641 cggccgggcg ttcgaactca tgaccctctg atcctcatcg gcgatctcga acagtctgta
    14701 ggcggcccgg ccagcctcgc cgcgccggac cgcccgaaag cgggaagggg tagacgagct
    14761 tcccgcctat ttcatgctct tgaccacctt ctccggcgcc ggctggtgat agatttcctg
    14821 ccaggcggcg agcagcgtgt ccctggtgat cggcgcggcg ggcagcgcga cataagcagg
    14881 cgccggcttg ccgatcagcg cataggcggc gagcttggct tcggtcacgc cttgatcgta
    14941 gggacgctgc gaggcgatgc ccttgatgaa ctccttcttg gccatcgcga tggcgacgtt
    15001 ctcgccgaga tcctcgcaga cgacggtgag atcgtcgcgg ccggcggcgc gcgcggcggc
    15061 catcacgccc tcggccggca cgtcccaaac ggcccagatc ccgttcaacc tcggatgctc
    15121 ggtgagcagg gccgaggcgg ccttttccgc gtcgcccgag aaatccggcc cgccgatgcc
    15181 ctgctcgccg acgatcttga tatcgggata gtcctgaagg atcgtctttt tgaatccgtc
    15241 atagcgctgg cgcgtgacga agaagtcggc ggcgtggaag acgaggccga tctcgccctt
    15301 gccgccgagc gccttggcca tgagatgggc cgcggcgacg ccgttgccgt aattgtcggc
    15361 cgataccgaa ctcacatatt ccttgccggc ggtgaagcct ttcgggacat tgtccatgaa
    15421 gaccagcttg acgcccttgt cggcggcggc cttgtacgcg gcggcggtcg ccacagcgtc
    15481 gacgggtatc gatacgatga tgtcgggctt ctgcgccatc acggtttcga tgtcggcgac
    15541 ctgcttttcc ggcttgaagc cggcgtcggt ggtggcgatg acgtcgatcc cttccgcggc
    15601 gaattgcgct ttgagcgcgg cctcctgcgc gttcgaccaa tcgttgccgc catagtgaaa
    15661 caccagagcc gccttcgcgt gcttggcctt caccttggcg agttcgtcgg cggtgagcgt
    15721 caccgtcgag gcggcggtcg gcttttcgcc gttcggcccg aggctgaaca cctggttctt
    15781 catttgcgcg agcgcggcgt ccggatcggt cgcataagcc ggcgcgccga aggcgccgat
    15841 cgtggcgatc gcggcgcagg ccgttaaaaa cttcgttctc atcatgacgt cctcccttgc
    15901 ctgtgtctct atccgggcga tttggcgagc gcgccgcgaa gaaagccgag gctcctcgcc
    15961 gcgccgtccc tcgggtcggg ccaggaatcg agctccgccg tgatccagcc gcgatagtcg
    16021 acctcggcaa gcgcatcgac gatcgccgcg atgtcgagat cgccgcgatc gagcggcgtg
    16081 aaagcgaagg gctctcgggt ccagcctttg aggtgtacgt acgaaatacg ccgcctgtgc
    16141 ttgcggatca tctcggcgac atcgccgccg gccgccgcca gatgggccgt gtcggggcag
    16201 aaatcgatcg aggtcagcgc gaatatttta tcgacctcgg ccggcccctc gacgatcgtc
    16261 gtcaaatgcg ggtgatagtg cgccttgagg ccgcgcgctt tggcgatggc gacgaccttt
    16321 tcgagcgctt cgcccagccg ttgatggtcg tccgccttca cgccgccggc gcgtttggcg
    16381 ccgccgccga cgacgagatg ctcggcgccg aaagccgcag cggcttcggc cgcgcgggtg
    16441 attctcgcca gctcctcggc gagaatgtca gggaaaatga aattggcgcc gcaatagacc
    16501 gcgacgagct tgagcccggc ccgatcgagc gcgccgccga gatcgctgcg ccgcccgtca
    16561 taatcgagaa gattgccgtc gaacaattcg acgccctcat agccgacggc gccgatctcg
    16621 gcgatcgccc ggcccatatc gccgaatgtc cggtaagcga gctgggcaat cgaggtgacg
    16681 ccgacagcgt cgccgccgag cgggccccag caatttgcgt gataggcgag cctcgccctc
    16741 atcgcgtcct ccctctgtcg ccggcggccg atctgcggcg cgcctcgcgt ctgtccttcc
    16801 ccgtcactgt gagatcggac gcgcgcagcg tcaacggaaa tatcggcaaa agcaaagcaa
    16861 cttttgctgg aaaattactt gattctgttg cttacggttt cgttttgtgg ttcaacatcg
    16921 cgccgcgccc gcggccgaat ggatccaagc ggccgatcag gccttcaggc gccctggcat
    16981 ggacggtggc gatcaaaaca ggcttcccgg ggctcggagc ccggcgcgtc gggtcgggcg
    17041 tccgcggacg cttgagaccg gcggcgccag cctcgccctc gtgctcaatc tgatccgcag
    17101 gggcgaagcg acgacgcggc tcgacctcga gcggaaatcg gagctcggcc gggccgctct
    17161 tgtcgaccgg cttggcaagc tgcaggcgct tggcctcgcg gcggagggcg atctcggcgc
    17221 ttccgacggc ggccgcgcgc cgcggcatat gcgcttcctt gccgaaacgg gcgcgatcct
    17281 catcgcttcg atcgagcgcg cgtcgatgtc ggtggcgctc gccgatcttt ccggccgcct
    17341 gatcgtcgaa catcacgaag cggtcgatct cgccgacggg ccggaagcca tcctcgaccg
    17401 ggtcaccaat cttttcatct ggctcatgga cgagcgcggc ggcaaggagc gcgtctgggc
    17461 gatgggcctg gctttgccgg agccggttct cgccgatacg aatgacggcg acgccttcgg
    17521 catcgtctcg ctcgacgctc tgcgctcctg gcgcggcttc gacttcgaat cggaactgtc
    17581 cttgcgcttc aaggcgccgt gctgggcgcg cggcggcgcg cagatgatga cgttcggcga
    17641 attcaaacat ggcgcctgtc aaggcgccgg cgatctcctc ttcgtgaagc tcggccattc
    17701 gatcggcgcc gggatggtct cggccggccg cctgcatcgc ggcgcgcaag gcctcgccgg
    17761 catgatcggc catgcgccga ccggcgagag cggcggcgcc cccgcctgcc attgcggcgc
    17821 caaaggctgc ctcgaagcgc tcgccagcgg cgaagccatt gcgcgcgaag cccggctggc
    17881 cgcgcaggac ggccgcagcc gctatctcgc cgatatggcc ggcctaaacg gcgaggtgac
    17941 ggcgacggat gtcggacacg gcgcacaact cggcgacgcc ttctgcgccg acctgctgtc
    18001 gcgctgcggc cggcttgttg gcgagagttt ggcgtctctg gtcaatttgc tgaatccggc
    18061 tttcgtcgtg ctcggcggca gcgtcgccga atccgccgac gtcctgctgg ccgcgttccg
    18121 cgaggcgatc taccggcagt cgcatcccct ggtgacgcgc gatctgcgga tcgtgcgctc
    18181 gcagatgggc gagaccgccg cgctgctcgg cgcggcgcat ctcgttctcg atgaggtctt
    18241 ctcgccggct tatttgagcg attggatcgc gcatggctcg ccgcggcggc gcccagcgtt
    18301 tctggaattc ttggaaaccg agcgaggcaa gcggcgcgcc tcggcgcctg cgccgcggcc
    18361 gccgcgc
//
LOCUS       DATGJW010000089         7587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_948955, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000089 DATGJW010000000
VERSION     DATGJW010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7587
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_948955"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VKU03_02695"
     CDS             <1..405
                     /locus_tag="VKU03_02695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLJ70198.1"
                     /translation="ELTRLAATLPRMINLALRVTEWKGDLVFLHEVAPGAADRSYGVQ
                     VAKLAGLPSAVVERARAILAELEAGKSSGAALKALDDLPLFTYAPPPVPAPPKRDPLR
                     EKLAALDPDDLSPREAQAALYALKQIMKEDEG"
     gene            402..1028
                     /locus_tag="VKU03_02700"
     CDS             402..1028
                     /locus_tag="VKU03_02700"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HLJ70199.1"
                     /translation="MNVYLATAIAAYLYVVAPGPAFLALFTLAASKGRAAGAWFICGH
                     LVGDVTWGALAFAAIIGANRIGPTLFEILGFGCGAYLIYLGGKAVMTKKGTNAPAVGS
                     RRPLIAGMAFGLTNPKSYPVAVAMFTAIAAPFAHQLTFADAPRLILAAFAGFLSGDLT
                     LIFAAGLPAVRRFFLRHGVIVTRVVGALFIAFGAKSISDAASGALRRA"
     gene            complement(1141..2592)
                     /locus_tag="VKU03_02705"
     CDS             complement(1141..2592)
                     /locus_tag="VKU03_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLJ70200.1"
                     /translation="MTGLRQDGLRSPRPPYRPPAPQPQPRPLGAFALLGALRRNPLEC
                     WARPHFEQPIVEGGLPVGHVLLVHEPAAIRHVLLENTANYQKDRLQRRVLSAGLSDGL
                     LSAEGEQWRVQRRALAPIFAHKQVRDFAPAMIEAGKAMMERWLGMGDDAEIDVAVEMT
                     RLTLDVIERTIFSDGLGRDAESIRLAMKVYFAAIGKISPLDLIGAPDFVPRLARWRAR
                     STLRFFEAAIDEVIATRRRLLAERPDAAPRDILTLLLNALDAESGARMSEAEVRSNIL
                     TFISAGHETTANALTWAMFLLSQSDEWRERIEAEAQREYSGPVEGLVDRLVETRAVIE
                     EAIRLYPPIAAISRVALGPDQLGDKRVRRGSLIVISPYVLHRHRLLWDRPDMFDPTRF
                     LGPARTTIDRFSYLPFGVGPRICIGHAFALHEATLVLAMAASRFSFALKEGARIWPML
                     RVTLRPENGLPMIIRRKAAPPRNDARRLETSAA"
     gene            complement(2815..3207)
                     /locus_tag="VKU03_02710"
     CDS             complement(2815..3207)
                     /locus_tag="VKU03_02710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70201.1"
                     /translation="MMKALVRVALGGALFSALAASSSAAAAHDIYTGVHGKNGQLCCG
                     GDDCASTVYRERGGRFEFLTREQEWVAIPEERITFLPIPGDPPSEDSHHAHLCYRSAT
                     EFDRKGPLSRNVFENIFLYCAFIPPGSI"
     gene            3786..4682
                     /locus_tag="VKU03_02715"
     CDS             3786..4682
                     /locus_tag="VKU03_02715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974713.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ70202.1"
                     /translation="MSAKRIVFTGGSGKAGRHAVPHLLAKGYKILNVDLKPLDLQGVN
                     TLVADLTDSGQAFNALTTHYGFEGYDEGKPPSAPDAVVHFAAIPRVLIEPDNKTYQVN
                     VMSTYNVIEAAMKLGVRKVVIASSETTYGVCFAEGDKDFKSFPLDEDYDVDPMDSYGL
                     SKVVNEKTARAFAMRYRADIYALRIGNVIEPHEYDRFPAFIADPMSRKRNAWSYIDAR
                     DLGEIVHCCLQKDGLGFQVFNATNDTITANIPTAEFLKRYCPDTPATRPLGEWEAPLS
                     NRKAREMLGFKEEHNWRKYVKG"
     gene            4829..5098
                     /locus_tag="VKU03_02720"
     CDS             4829..5098
                     /locus_tag="VKU03_02720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70203.1"
                     /translation="MRNFFLVGAAGLCLALGAASGALAMGNMGGPSDAWGSPYVLYIP
                     QTFQPPPGWGPLEGRSAYEGRAAVDCHGDRACERRLRKAAPAPAQ"
     gene            5209..5481
                     /locus_tag="VKU03_02725"
     CDS             5209..5481
                     /locus_tag="VKU03_02725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70204.1"
                     /translation="MPKILLAAGALLASALAASSAYAIGGGPTPPWASPYAILEPQTV
                     APEAAAPFAAVEGRSAYIGGKRDGEWRGGDRERDRRGHRRAPAPER"
     gene            5696..7099
                     /locus_tag="VKU03_02730"
     CDS             5696..7099
                     /locus_tag="VKU03_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLJ70205.1"
                     /translation="MRRREGRLAPAAVAALTSDLAARFGDKLATGEAVREQHGHTLTW
                     TANQPPDAVVFAESVEDVVDAVKLCAKHDAPVIAFGTGTSLEGHLNAPHGGVSIDVSR
                     MNRVVDVHAEDLDCVVEPGVTRKALNEYLRDTGLFFPIDPGADASIGGMTATRASGTN
                     AVRYGTMKDNALSLKVVTADGAIVKTASRAKKSSAGYDLTRLFVGSEGTLGVIVEITL
                     RLYGAPEAMSAGHCPFKTVRGACDAVIATIQSGIPVARIELLDALQVKACNAYSKLSL
                     PETPMLFVEFHGSAASVAEQSERFGEIAAEFAGGPFAWATKPEERSKLWQARHDVYWA
                     TLPLRPGAKLFATDVCVPISRLADCVEETQRDIRESGLIAPIVGHVGDGNFHTSPLID
                     VNDPADVARGEAFSSRLVHRALAMGGTCTGEHGVGQKKARYLEEEHSAATLDLMRRVK
                     AAFDPRNLMNPGKIFTE"
     gene            7347..>7587
                     /locus_tag="VKU03_02735"
     CDS             7347..>7587
                     /locus_tag="VKU03_02735"
                     /inference="COORDINATES: protein motif:HMM:NF017021.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HLJ70206.1"
                     /translation="MRVFLTLVALVLIAVLTTALVGPYFIDWSQHRAAVEKELSAALG
                     RPVVVKGPIKAALLPVPYLKLADVAVGGADGGADLS"
BASE COUNT         1281 a         2582 c         2434 g         1290 t
ORIGIN      
        1 gaattgaccc ggctcgccgc gaccctgccg aggatgatca acctcgcctt gcgcgtcacc
       61 gaatggaagg gcgacctcgt cttcctgcac gaagtcgcgc ccggagcggc ggatcgctcc
      121 tatggcgtgc aggtcgccaa gctcgccggc ctgccgagcg cggtcgtcga gcgcgcccgc
      181 gccattctcg ccgagttgga ggcgggcaaa agctccggcg ccgcgctcaa ggcgctcgac
      241 gatctgccgc tcttcaccta tgcgccgccg cctgtcccgg cgccgcccaa gcgcgaccct
      301 ttgcgcgaga agctcgcggc gctcgatcca gacgatcttt caccgcgcga ggcgcaggcg
      361 gcgctctatg cgctgaagca gatcatgaaa gaggacgagg gatgaacgtt tacctggcga
      421 cggcgatcgc cgcctacctc tatgtcgtcg cgcccggacc ggcttttttg gcgctgttca
      481 cgcttgccgc gtcgaagggc cgcgccgccg gcgcgtggtt catctgcggc catctggtcg
      541 gcgacgtcac ctggggcgcg ctggctttcg ccgcgatcat cggcgcgaac cggatcggcc
      601 cgactctgtt cgagattctc ggcttcggct gcggcgccta tctgatctat ctcggcggca
      661 aagcggtcat gacgaaaaag ggtacgaacg cgccggcggt cggctcgcgc cggccgctca
      721 tcgcgggcat ggcgttcggc ctgaccaatc cgaaatccta ccccgtcgcg gtcgcgatgt
      781 tcaccgccat cgccgcgcct ttcgcccatc agctcacttt cgccgacgcg ccgaggctga
      841 tcctcgccgc tttcgccggc tttctcagcg gcgacctgac gctgattttc gccgccggcc
      901 tgcctgcggt caggcgcttc ttcctgcgcc atggcgtcat cgtcacgcgc gtcgtcggcg
      961 cgctgttcat cgctttcggc gcgaaatcga tctcggacgc ggcgagcggg gcgttgcgga
     1021 gggcgtaaac gccccgcgag ccatgacggc gagcattaac tgagcgccaa gtaagcgggt
     1081 atccagtcct gaggctctct ggattcttgc ttgcgcggga atgacattgg cgccgcattc
     1141 ctacgccgcc gacgtctcca gccgtctcgc gtcgttgcgc ggcggcgcag ccttgcgccg
     1201 gatgatcatc ggcaggccgt tctcgggacg caacgtcact cgcagcatgg gccaaatcct
     1261 cgcgccttct ttcagcgcga agctgaaccg gctcgccgcc atggcgagga cgagcgtcgc
     1321 ttcgtgcaag gcgaaggcgt ggccgatgca gatgcgcggc ccgacgccaa agggcaggta
     1381 cgagaagcgg tcgatcgtcg tccgcgcggg cccgagaaag cgcgtgggat cgaacatatc
     1441 cggccggtcc caaagcaggc ggtggcgatg cagcacataa ggagagatga cgatgagcga
     1501 accgcgcctg acgcgcttat cgcccaattg gtcgggaccg agcgccaccc ggctgatcgc
     1561 ggcgatcggc ggataaagcc ggatcgcctc ctcgatcacc gcgcgcgttt cgacgaggcg
     1621 gtcgacaagc ccctcgactg gccctgaata ttcgcgctgc gcctccgctt cgatccgctc
     1681 gcgccactcg tccgattgcg acaggaggaa catcgcccag gtcagcgcat tggcggtggt
     1741 ctcgtggccg gcggagatga aggtcagaat gttggagcgg acttccgcct cgctcatccg
     1801 cgcgccgctc tcggcgtcga gcgcgttcag gagcagggtg agaatatcgc gcggcgccgc
     1861 gtccggccgc tcggcgagca ggcgccgccg cgttgcgatc acctcgtcga tcgccgcctc
     1921 gaaaaagcgt agggtcgagc gggcgcgcca gcgcgcgagg cgcggcacga aatccggcgc
     1981 gccgatcagg tcgagcgggc tgattttgcc gatcgcggcg aaatagacct tcatcgccaa
     2041 gcggatgctt tccgcgtcgc ggccgaggcc atcggaaaag atggtccgct cgatcacgtc
     2101 cagcgtcagc cgggtcatct cgaccgcgac gtcgatctcg gcgtcgtcgc ccatgccaag
     2161 ccagcgctcc atcatggcct tgccagcttc gatcatggcc ggcgcgaaat cccgcacctg
     2221 cttgtgcgcg aagatcggcg ccagcgcccg cctttgcact cgccattgct cgccttccgc
     2281 gctgagcagg ccatcgctca agccggcgga aagcacccgg cgctgcagcc gatccttctg
     2341 gtaattggcc gtattttcca gaagaacgtg gcggattgcg gccggctcgt ggaccagcag
     2401 cacgtgcccg accggcaagc cgccctcgac gatcggctgc tcgaaatgcg ggcgcgccca
     2461 gcattcgagc gggttgcgcc gcagcgcgcc cagcagggcg aacgcgccga gcggccgcgg
     2521 ctgcggctgc ggcgccggcg ggcgataagg cgggcgcggc gagcgcaagc cgtcctggcg
     2581 gaggcccgtc atgctatttc cccgatcatc ttcgtcaatt agccattaac gcattgtggc
     2641 gtcaatcggt tcccgttctc gctccggttg atccgagcca gtttgagaac gcgcctggaa
     2701 gataggaggg gccaatattg gcgccggacc agaggtcaca ggccggcgag ccgcggcgaa
     2761 gcgatcgcac tcgtggaggc ggcgagggcc tcagcttccg ctttcggccg ccgcttagat
     2821 cgagcccggc ggaatgaacg cgcaatagag aaagatattc tcgaagacgt tgcgcgataa
     2881 cggtcccttg cggtcgaact ccgtcgccga gcgatagcag agatgcgcgt gatgggagtc
     2941 ttcgctcggc ggatcgcccg gaatcggcag gaaggtgatg cgttcttccg gaatggcgac
     3001 ccattcctgc tcgcgggtca agaattcgaa cctgccgccg cgctcgcgat agacggtcga
     3061 agcgcaatcg tcgccgccgc agcaaagctg gccgttcttg ccgtgaacgc cggtatagat
     3121 atcatgcgcg gcggccgccg aggaggaagc cgccagcgcc gaaaagaggg cgccgcccag
     3181 agcgacgcga accagagctt tcatcatcgt caaccgctcc tactggcggc cgatcgatcg
     3241 aaacgtcgtc aagcgatgcg atcgagccgc gcgccgatcg ccctccgagg acgccgaact
     3301 cctcccgaat cgcggcgcgg cgacagggcc gcgcgaattc ggcgcgttaa aacgcgtcga
     3361 caaagccgcg ccccctcgac gcgccgcttc gctgatgccg cttgaaagac gtctgccccg
     3421 cctctggccg gacgcgatcg aagcgcattc cggcccatcg gcgcctcgcc aagcgagcgc
     3481 cgattcgcgg gcgacatcag aatcctcaac ggacgacgcg tcaagagccg cgtcggttct
     3541 atccccttct tccccgcttc gcggcgagga aggcgggcga agcgccggac gaggggccgc
     3601 gcagaactat gaaagtacgg cgcgccgccg ttgcaagcca agcgaagcat tcttttcgcg
     3661 gcgcgccggc ccggccgctt ggggcgaggg tttcgctctc ttttctgtga gcgcccttgc
     3721 tatggccgca gaatgcgatc gcgcttataa ccccgccaat catcgcttga ggtcaaggag
     3781 tcccaatgtc cgccaaacgc atcgtcttta ccggcggctc cggcaaagcc ggacggcatg
     3841 ccgtgccgca tctcctggcg aagggctaca agatccttaa cgtcgatctg aagccgcttg
     3901 accttcaggg cgtcaatacg ctggtcgccg atctgaccga tagcggccaa gccttcaacg
     3961 cgctgaccac ccattacggt ttcgaagggt atgacgaggg caagccgcct tcggcgcccg
     4021 acgcggtcgt gcatttcgcc gccattccgc gcgtgctgat cgagccggac aataagacgt
     4081 atcaggtcaa tgtgatgtcg acctacaatg tcatcgaagc ggcgatgaag ctcggcgtcc
     4141 gcaaagtggt gatcgcatcg agcgagacga cctacggcgt ctgcttcgcc gagggcgaca
     4201 aggacttcaa gagcttcccg ctcgacgaag attacgacgt cgacccgatg gattcgtacg
     4261 gcctctccaa ggtcgtcaac gaaaagaccg cccgcgcctt cgccatgcgc tatcgcgccg
     4321 atatctacgc cctgcgcatc ggcaacgtga tcgaaccgca cgaatacgac cgctttcctg
     4381 ctttcatcgc cgatccgatg tcgcgcaagc gcaacgcctg gtcctatatc gacgcccgtg
     4441 atctcggcga gatcgtccat tgctgcctgc aaaaagatgg gctcggcttc caggtcttca
     4501 acgcgaccaa cgacacgatc accgccaaca ttccgactgc ggagttcctg aagcgatact
     4561 gccccgatac gccggcgacg cggccgctgg gcgaatggga agcgccgctg tcgaaccgca
     4621 aggcgcgcga aatgttggga ttcaaggaag agcataattg gcgaaaatat gtgaaagggt
     4681 gaggccgcgg ctcggcggaa cgaataatca tttacgcgcg attgtttacc gccgcggccg
     4741 accgaagcgt ccggtccgct cgcctcggtg gcggcaacgc gccggccgcc tatatgaagc
     4801 ggcgaggcga aagcgaagga gcagagtcat gaggaatttc tttctagtcg gcgcggccgg
     4861 cctgtgtctc gcgcttggag cggcgagcgg cgccctggcg atgggtaata tgggcggccc
     4921 gtcggacgcg tggggctcgc cttatgtgct ctatattccg cagaccttcc agccgccgcc
     4981 gggctgggga ccgctggagg ggcgctcggc ctatgaaggc cgcgccgcgg tcgactgcca
     5041 tggcgacaga gcctgcgagc ggcgcctgag gaaagccgcg cccgcgccgg cgcaatagtt
     5101 caaaagcccg cgaatttctt tcggccggcg ctgtggcctt cgccacagcg ccgcgacgct
     5161 tttggcgcaa taagtcgcgc cgaaatcgct tcaagaaagg aattcgccat gccgaagatt
     5221 cttctcgccg ccggcgcatt gctcgcctcc gccctcgccg cgtccagcgc ttacgcgatc
     5281 ggcggcggcc cgacgccgcc ttgggcgtcg ccctacgcga ttcttgagcc gcagacggtc
     5341 gcgccggaag ccgccgcgcc gttcgcagcg gtcgaagggc gctccgccta tatcggcggc
     5401 aagcgcgacg gcgaatggcg cggcggagat cgcgagcgcg accgccgcgg ccatcgccgc
     5461 gcccccgcgc ccgagcggtg acgcggagcg cgccgagcgg cgcgccgttc ccgtcatgcg
     5521 agcgacgcga agcaatccac tcccaaggcg cgctcacgaa tggattgctt ggtggcttcg
     5581 ctccccggaa tgacggtcgt accttttcga cccagatttg ctcccttcgc ggcggcgctt
     5641 tatcttccgc gccgagagga accccgccgt gaacgactcc cccccgccct tccaaatccg
     5701 ccgccgcgag ggccgtcttg cgcccgccgc ggtcgcggcc ttgaccagcg acctcgccgc
     5761 gcgcttcggc gacaagctcg cgaccggcga agccgtgcgc gagcagcacg gccacacgct
     5821 gacctggacc gccaatcagc cgcccgacgc ggtcgtcttc gccgagtcgg tcgaggacgt
     5881 ggtcgacgcg gtcaagctct gcgccaagca cgacgcgccg gtcatcgcct tcggaaccgg
     5941 cacctcgctc gaaggccatc tcaacgcgcc gcatggcggc gtctcgatcg atgtttcgcg
     6001 gatgaatcgc gtcgtcgacg ttcacgccga ggatttggat tgcgtcgtcg agccgggcgt
     6061 cacgcgcaag gcgctcaacg aatatttgcg cgacaccggc ctgttctttc cgatcgaccc
     6121 cggcgccgac gcttcgatcg gcggcatgac ggcgacgcgc gcctccggca ccaatgccgt
     6181 gcgctacggc acgatgaagg acaatgccct ctcgctcaag gtcgtcaccg ccgacggcgc
     6241 gatcgtcaag acggcgagcc gcgccaagaa atcctcggca gggtacgacc tgacgcggct
     6301 cttcgtcggc tcggaaggca cgctcggcgt gatcgtcgag atcacgcttc ggctctacgg
     6361 cgcgccggaa gccatgtcgg ccggccattg cccgttcaag accgtgcgcg gcgcgtgcga
     6421 cgccgtgatc gcgacgatcc aatcgggcat tcccgtggcc cggatcgaat tgctcgacgc
     6481 ccttcaggtc aaagcctgca acgcctattc gaaactctcc ttgcccgaaa cgccgatgct
     6541 gttcgtcgag tttcacggca gcgcggcgag cgtcgccgag caatccgaac gcttcggcga
     6601 gatcgccgcc gaattcgccg gcgggccgtt cgcctgggcg accaagccgg aggaacgctc
     6661 caaactctgg caggcgcggc acgacgtcta ttgggcgacc ctgccgctac ggcccggcgc
     6721 caagcttttc gccacggatg tctgcgtgcc gatctccagg ctcgccgatt gcgtcgagga
     6781 gacgcagcgc gacattcgcg aatccggcct catcgcgccg atcgtcggcc atgtcggcga
     6841 cggcaatttt cacaccagcc cgctgatcga cgtgaacgat cccgccgatg tcgcgcgcgg
     6901 cgaagctttt tccagccgcc tcgtccatcg cgctttggcg atgggcggca cctgcaccgg
     6961 cgagcacggc gtcggccaga aaaaagcgcg ctatctcgaa gaggagcatt ccgcggcgac
     7021 gctggatttg atgcgccggg tcaaggccgc cttcgatccg cgcaatctga tgaatccggg
     7081 aaagattttc accgagtgag ggcgcttctt ctccccgtga agcgtaagcg gaacaggggg
     7141 accatgcgaa gcatggtgga gggggctcgg cggttgagga ttatcttgaa ccgccgcagc
     7201 cccctccacc gcgctacgcg cggtccccct cccccgcctt cagcgaggga ggataacgac
     7261 atcccttccg tcaccctcgc gccacgaagt cggctattgt gcgcccggaa ccggccccgg
     7321 ctttgctctt gaattgacgt tccgctttgc gcgtattcct gacccttgtc gctctcgtgc
     7381 tgatcgccgt gctgaccacg gctctggtcg gtccctattt catcgactgg tcgcagcacc
     7441 gcgcggcggt cgaaaaagag ctgtcggcgg ctttgggccg tcccgtcgtc gtcaagggtc
     7501 cgatcaaggc ggcgctgctg cctgtcccct atctgaagct cgccgatgtc gcggtcggcg
     7561 gcgcggatgg cggcgccgat ctttcct
//
LOCUS       DATGJW010000090         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1260473, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000090 DATGJW010000000
VERSION     DATGJW010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1260473"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..872
                     /locus_tag="VKU03_02740"
     CDS             <1..872
                     /locus_tag="VKU03_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLJ70207.1"
                     /translation="DAEVALAHAKKAGGDRAELFVPAMRGYRADRASLESDLRRAVDR
                     REIKVVFQPIVRLDDRTVAGFEALLRWDHPRFGPINPSEFIPIAEESGVLVNIGNYAL
                     EQTARELAAWQRALDVNPPIFASLNVFSRQLLRHDLLADLKAVLTRHDVQRGTLKLEL
                     TEGLVMENPEFAAVLLQRVHEVGAGLALDEFGAGYSSLGHLQRYRFDTLKIAASLVRQ
                     NSVGARPAILRSIVTIAHDLEMDVVAEGAETESDAVELSQLGCEFAQGSAFGKPMTAN
                     EARRLMGAVGVPA"
     gene            1055..>2853
                     /gene="dnaG"
                     /locus_tag="VKU03_02745"
     CDS             1055..>2853
                     /gene="dnaG"
                     /locus_tag="VKU03_02745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003896 - DNA primase activity [Evidence
                     IEA];
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA primase"
                     /protein_id="HLJ70208.1"
                     /translation="MRFPPSFLDEIRARVPVSEVVGKRVKLRKQGREWAGLSPFNAEK
                     TPSFFVNDQKGFYHDFSSGRHGDAFRFLMETEGVSFPEAVERLAGMAGLAMPVVSRDS
                     EEAEKKRASLHEVLALAARIFEDNLQSSFGAKARGYLADRRIDAKIQRDFGLGYAAAE
                     RFALRDALAAKGVGADQMIAAGLLVHGEDIAVPYDRFRDRIMFPIHDRSGRVIGFGGR
                     AMEAGAPAKYLNSPETELFHKGAILFNHHRARKSAHDKGEIVAVEGYVDAIQMSAAGF
                     PQTVAPLGTALTADQCELLWRMAGEPILCFDGDKAGRKAAFRAIDVALPLIGPGKSLR
                     FALLPEGQDPDELIRAAGPGAVGEALQAAKPLAEMLFLREIEGQSFDTPERRAALERR
                     LRELVGLIADEPLRRHYREDMETRLAGLFGGGRRPAYRGPARGGERGRRPFGDPGPRL
                     GVASEPLPGPPPPAGGAREAPRDVAILAIAISHPDLLARHAEEIAALEFANRRLAALR
                     DRLLDLAAEPSAAALAEALTAAGLAEERDRILRLAAKGPDWRLLKPEAPEGDVELVLR
                     QSLALHRRARALNKELKLAEKALAEDFSEQNFA"
BASE COUNT          463 a          956 c          968 g          466 t
ORIGIN      
        1 aagacgcgga agtcgctctc gctcacgcca aaaaggccgg cggcgaccgc gccgagcttt
       61 tcgtgccggc gatgcgcggc tatcgcgccg accgcgccag ccttgaaagc gacttgcgcc
      121 gcgccgtcga ccgccgcgag atcaaggttg tctttcagcc gatcgtgcgg ctcgacgatc
      181 gcaccgtcgc cggcttcgag gcgctgctgc gctgggacca tccgcgcttc gggccgatca
      241 atccgtccga attcatcccc atcgccgagg agagcggcgt cctcgtcaat atcggcaatt
      301 acgcgcttga gcagaccgcc cgcgaactcg ccgcctggca gcgcgctctc gacgtcaatc
      361 cgccgatttt cgccagtctc aatgtctttt cgcggcaatt gttgcgccac gacctgctcg
      421 ccgacttgaa ggccgtgctg acccgccatg acgtgcagcg cggcacgctg aagctcgaat
      481 tgaccgaggg actggtcatg gagaatccgg aattcgcggc cgtgcttctc cagcgcgtcc
      541 acgaggtcgg ggcaggcttg gcgcttgacg aattcggcgc cggctattcg tcgctcggcc
      601 atttgcagcg ctatcgtttc gacacgctca aaatcgccgc gtcgctggtg cgtcagaaca
      661 gcgtcggtgc gcggccggcg atcctgcgct cgatcgtcac catcgcgcat gatctggaaa
      721 tggacgttgt ggcggaaggc gctgagaccg aatccgacgc cgtcgaattg tcgcagctcg
      781 gctgcgaatt cgcgcaaggc tcggctttcg gcaagccgat gaccgcgaac gaagcgcgcc
      841 gcctgatggg cgcggtcggc gtgccggcgt aacgctgtgg aagtcgggga gaagatcgtg
      901 agacgcttgc gcttgaaggc gcaggcgcct gtggtcgagg ttcaccatta accgtcattg
      961 cgaggagcga agcggcgaag caatccagcg gcaaggctga ggcggttgct gtggattgct
     1021 tccttcgctc gcaatgacgc cctgaggcga agcgatgaga tttccgccgt cctttctcga
     1081 tgagatccgg gctcgcgtcc cggtctccga ggtcgtcggc aaacgggtca aactgaggaa
     1141 gcaggggcgc gaatgggccg gcctgtcgcc gttcaatgcg gaaaagacgc cgtccttctt
     1201 cgtcaacgac cagaagggct tctaccacga tttctcctcc ggccggcatg gcgacgcgtt
     1261 ccgcttcctg atggagaccg agggcgtcag cttcccggaa gcggtcgagc gcctcgccgg
     1321 catggccggt ttagccatgc cggtcgtcag ccgcgacagc gaggaagcgg agaagaagcg
     1381 cgcatcgctg catgaggtcc tcgctctggc ggcgcgcatt ttcgaggaca acctgcaatc
     1441 gagcttcggc gccaaggcgc gcggctatct cgccgaccgg cggatcgacg ccaaaatcca
     1501 gcgcgatttc ggcctcggct atgcggccgc cgagcgcttc gccctgcgcg acgcgctggc
     1561 cgccaagggc gtcggcgccg atcagatgat cgccgccggc cttctcgtcc atggcgagga
     1621 catcgccgtc ccttacgacc gcttccgcga ccggatcatg ttcccgatcc atgaccgctc
     1681 cggccgggtg atcggctttg gcgggcgcgc catggaggcc ggcgcgccgg ccaaatatct
     1741 gaactcgccg gaaaccgagc tcttccataa gggcgcgatt ctgttcaatc accatcgcgc
     1801 ccgcaaaagc gcccatgaca agggcgagat cgtcgccgtc gaaggctatg tcgacgccat
     1861 ccagatgagc gcggcgggct tcccgcaaac ggtcgcgccg ctcggcacgg ctttgaccgc
     1921 cgaccagtgc gaattgctgt ggcgcatggc tggcgagccg atcctctgct tcgacggcga
     1981 caaagccggc cgcaaggcgg cgttccgggc gatcgacgtc gcgctgccgc tgatcgggcc
     2041 gggcaagagc ctgcgcttcg ctttgctgcc ggaaggccag gacccggacg aattgatccg
     2101 cgccgccggc ccaggcgcgg tcggggaggc gctgcaggcg gccaaaccgc tagcggaaat
     2161 gctttttctc cgcgagatcg aggggcagtc gttcgacacg ccggagcgcc gcgccgccct
     2221 ggagcgacgc ctgcgcgagc tggtcgggct gatcgccgac gagccgctgc gccggcatta
     2281 ccgcgaggac atggagacgc ggctcgccgg ccttttcggc ggcggccggc ggccggctta
     2341 caggggcccg gcgcgaggcg gtgaaagggg gcgccggccc ttcggcgatc ccggtccgcg
     2401 cctcggcgtg gcgagcgagc cgctgccggg cccgccgccg cccgctggcg gcgcgcggga
     2461 agcgccgcgc gacgtcgcga tcctagcgat cgcgatcagc catcccgatc tcctggcgcg
     2521 ccacgccgag gagatcgcgg cgctcgaatt cgccaaccgg cgccttgccg ccttgcgcga
     2581 ccgcctgctc gacctcgccg ccgagccaag cgcggcggcg cttgccgagg cgctgaccgc
     2641 cgccggcctc gcggaggagc gcgaccgcat tttgcgcctc gccgcaaaag ggccggattg
     2701 gcgccttctc aagccggagg cgcccgaggg cgacgtggag ctggtcttgc ggcaaagctt
     2761 ggccttgcat cgcagggcaa gggcgctaaa taaggagctt aaactggccg aaaaagcgct
     2821 tgccgaggat ttcagcgagc agaatttcgc cag
//
LOCUS       DATGJW010000091         2939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_15430, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000091 DATGJW010000000
VERSION     DATGJW010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2939
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15430"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VKU03_02750"
     CDS             complement(<1..591)
                     /locus_tag="VKU03_02750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isocitrate lyase/phosphoenolpyruvate mutase
                     family protein"
                     /protein_id="HLJ70209.1"
                     /translation="MSATIEEKRARFAELHRGPGCFVMPNPWDVGSAIYLSTLGFKAL
                     ATTSRGAAFSAGRPDGGLQREEALAHIGALVAASDLPFNADFENGFADNRDRLAESVR
                     LCVETGVAGLSIEDYSGDPAKPFYDFDEAVARVRTARAAIDATGAKVILTARSEIVLH
                     GHEGGLREALRRLAAFAEAGADALYAPGLRKPEDVAE"
     gene            complement(588..1307)
                     /locus_tag="VKU03_02755"
     CDS             complement(588..1307)
                     /locus_tag="VKU03_02755"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HLJ70210.1"
                     /translation="MAQDTIGPLRRPIGPLVDARPAKRPERVTMEGRFITLTPLDAAA
                     HGDALFEAANGGEKDQVWDYLFDGPYADRAAFRANLEAKAKSEDPLIFAIIDRKSGKP
                     VGYQTLMRIEPAHRVIEIGNVLYTPAMQRTPGATEAQYLFARYVFDELGYRRYEWKCN
                     ALNAPSRRAAQRLGFTFEGVFRQHMIVKGRNRDTAWFSMLDHEWPARRAAFERWLAPD
                     NFDKAGRQIVSLSSLNGAPSS"
     gene            complement(1318..>2939)
                     /locus_tag="VKU03_02760"
     CDS             complement(1318..>2939)
                     /locus_tag="VKU03_02760"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="IlvD/Edd family dehydratase"
                     /protein_id="HLJ70211.1"
                     /translation="PCNRHHLELAKRVRDGIVAAGGIPFEFPVHPIQETGKRPTAALD
                     RNLAYLGLVETLYGYFIDGVVLTTGCDKTTPACIMAAATVNLPAIVLSGGPMLNGWHN
                     GERTGSGTIVWKAREELAAGKIDYEQFMELVTSSAPSVGHCNTMGTATTMNSLAEALG
                     MSLPGCAAIPGPYRERGQIAYETGKRIVEMVWEDLKPSDILTREAFENTIVVNSAIGG
                     STNAPIHINAIARHIGVKLSIEDWETIGHAVPLLVNLQPAGKYLGEEFHRAGGVPAVV
                     NELMKKKLIRKDALTVNGKSIGENCKGRESGDPDVIFPIDKPLMKDAGFIVLKGNLFD
                     SAIMKTSVIGEEFRKRYLSNPKDPEAFEGRAIVFDGPEDYHHRIDDPKLKIDEHCMLF
                     IRGAGPIGYPGGAEVVNMQPPAALIKKGVTALPCIGDGRQSGTSGSPSILNAAPEAAV
                     GGGLALLRTGDRVRIDLRKRTANILISDEELHQRRADYEKHGYAYPKSQTPWQEIQRG
                     LIEQFDEGMVLKPAVKYQRVAQTMGVPRDNH"
BASE COUNT          480 a          962 c          953 g          544 t
ORIGIN      
        1 ctccgcgaca tcctcgggct tgcgcaagcc cggcgcgtag agggcgtccg cgcccgcttc
       61 ggcgaaggcg gcgaggcggc gcaacgcctc ccgcagcccg ccttcatggc cgtgcagcac
      121 gatctcgctg cgcgccgtca aaatcacctt ggcgccggtc gcgtcgatcg ccgcccgcgc
      181 cgtcctcacc cgcgccacgg cctcgtcgaa atcgtagaaa ggcttggccg gatcgccgga
      241 atagtcctcg atcgacaggc cggcgacacc ggtttcgacg cagagccgca cgctttcggc
      301 gagccggtcg cgattatccg caaagccgtt ctcgaaatcg gcgttgaacg gcaggtcgct
      361 cgccgcaacc agcgctccga tatgggcgag cgcctcctcg cgctgcaagc cgccgtccgg
      421 ccggcccgcg gaaaacgcgg cgccgcggct ggttgtcgcc agggccttga agccgagcgt
      481 cgacaggtag atcgcgctgc cgacgtccca aggattgggc atgacgaagc agcccggccc
      541 gcgatgcagc tcggcgaaac gcgctctctt ttcttcgatt gtcgcgctca tgaggacggg
      601 gctccgttca gcgacgaaag ggagacgatc tggcgcccgg ctttatcgaa attgtccggc
      661 gcgagccagc gctcgaacgc ggcgcggcgc gccggccatt cgtgatcgag catcgagaac
      721 caggccgtgt cgcgattgcg gcccttgacg atcatgtgct ggcggaagac gccttcgaag
      781 gtgaagccga ggcgctgcgc cgcccgccgc gacggcgcgt tgagcgcgtt gcatttccat
      841 tcgtagcgcc gatagccgag ctcgtcgaac acgtagcgcg caaaaagata ttgcgcctcc
      901 gtcgcgcctg gcgtgcgctg catggcgggc gtgtagagta cgttgccgat ttcgatgacg
      961 cgatgggcgg gctcgatgcg catcaaagtc tgatagccga ccggcttgcc gctcttcctg
     1021 tcgataatcg cgaaaatcag cgggtcctcg gatttcgcct tggcttcgag gttcgcgcga
     1081 aaggcggcgc gatcggcgta aggcccgtcg aagagatagt cccagacctg gtccttctcg
     1141 ccgccattgg cggcctcgaa caaagcgtcg ccatgcgccg ccgcgtcgag cggcgtcaag
     1201 gtgatgaatc gcccctccat cgtgacgcgc tccggccgct tggcaggccg cgcatcgaca
     1261 agtgggccaa tgggccggcg cagcggcccg atcgtgtcct gagccatgtc gccccgctta
     1321 gtgattgtcg cgcggaacgc ccatcgtctg agcgacgcgt tgatatttca ccgccggctt
     1381 cagcaccatg ccttcgtcga attgctcgat caggccgcgc tgaatctcct gccagggcgt
     1441 ctgactcttc ggataggcgt agccgtgctt ttcataatcg gcgcggcgct gatgaagctc
     1501 ctcgtcggag atcagaatat tggcggtgcg ctttctcaaa tcgatgcgca cgcgatcgcc
     1561 ggtgcgcagc agcgcgaggc cgccgccgac cgccgcctcc ggcgcggcgt tgaggatcga
     1621 gggcgagccc gacgtgccgg attggcggcc gtcgccgatg cagggcagcg ccgtgacgcc
     1681 ctttttgatc aaagccgccg gcggctgcat gttgacgacc tcggcgccgc ccggatagcc
     1741 gatcggcccc gccccgcgaa tgaacagcat gcaatgttcg tcgatcttga gcttcggatc
     1801 gtcgatacgg tggtgataat cctccggccc gtcgaaaacg atggcgcggc cctcgaacgc
     1861 ttcggggtcc ttcgggttgg acaaatagcg cttgcggaac tcctcgccga tcacgctggt
     1921 cttcatgatc gcgctgtcga agagattgcc tttcagcacg atgaagccgg cgtctttcat
     1981 cagcggctta tcgatcggga agatcacgtc cggatcgccg ctctctctgc ctttgcaatt
     2041 ctcgccgatc gacttcccgt tgacagtgag cgcgtccttg cggatgagct tcttcttcat
     2101 caactcgttg acgaccgccg gcacgccgcc ggcgcggtgg aactcctcgc cgagatattt
     2161 gccggccggt tgcagattga cgagcagcgg cacggcatgg ccgatcgtct cccaatcctc
     2221 gatgctgagc ttcacgccaa tatggcgggc gatggcgttg atatgaatcg gcgcattggt
     2281 cgagccgccg atcgcgctat tgacgacgat cgtgttctcg aacgcctccc tcgtcagaat
     2341 gtcggagggc ttcaaatcct cccagaccat ctcgacgatg cgtttccccg tttcgtaggc
     2401 gatctggccg cgctcgcgat aagggccggg gattgccgcg cagccgggca ggctcatgcc
     2461 cagcgcttcg gcgagggagt tcatcgtggt cgccgtgccc atcgtgttgc aatggccgac
     2521 cgacggcgcc gaggaggtca ccagctccat gaattgctcg tagtcgatct ttccggcggc
     2581 gagctcttcg cgcgccttcc aaacgatcgt gcccgagccg gtgcgctcgc cgttatgcca
     2641 gccgttgagc atcggcccgc cggaaaggac gatcgccggg agattgaccg tcgccgccgc
     2701 catgatacag gccggcgtcg tcttgtcgca gccggtggtc agcaccacgc cgtcgatgaa
     2761 atagccgtaa agcgtctcga ccaggccgag ataggcgaga ttgcggtcga gcgccgcggt
     2821 cggccgcttg ccggtctcct ggatcggatg gaccggaaac tcgaacggga tgccgccggc
     2881 cgcgacgatg ccgtcgcgca cgcgcttggc gagttcgaga tgatggcggt tgcagggcg
//
LOCUS       DATGJW010000092         5646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_949927, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000092 DATGJW010000000
VERSION     DATGJW010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_949927"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(266..946)
                     /gene="trmD"
                     /locus_tag="VKU03_02765"
     CDS             complement(266..946)
                     /gene="trmD"
                     /locus_tag="VKU03_02765"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HLJ70212.1"
                     /translation="MFSATLLTLFPEMFPGPLGLSLAGDALQRGLWSLETLNIRAHGI
                     GRHRAVDDTPAGGGPGMVMRADVLAASLDAALQPDDPRPRLIMSPRGERLSQARVKQW
                     AAGPGLVIVCPRFEGVDERVIEARNLIEVSIGDYILSGGEIAALALLDACVRLIPGVM
                     GKAESGAEESFESGLLEYPHYTRPREWEGRAIPDVLLNGDHARIAEWRREQAEKLTKA
                     RRPDLLKD"
     gene            complement(1384..1926)
                     /gene="rimM"
                     /locus_tag="VKU03_02770"
     CDS             complement(1384..1926)
                     /gene="rimM"
                     /locus_tag="VKU03_02770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921925.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HLJ70213.1"
                     /translation="MAPPRLVAVGMFGAPHGVRGEVRVKAYTADPKSLAAYGELTDVA
                     GARRFKISALRPLKDDMVVVRLDGVGDRGAAAALTGTELFARRENMPPPDEDEFYHAD
                     LVGLRAETAAGEEIGRVVALNNYGAGDILEVAPKGGGETLLFPFTKAVAPEVDIAGGR
                     IVIAPPREIEGGGERRLHED"
     gene            complement(1933..2301)
                     /gene="rpsP"
                     /locus_tag="VKU03_02775"
     CDS             complement(1933..2301)
                     /gene="rpsP"
                     /locus_tag="VKU03_02775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HLJ70214.1"
                     /translation="MSLKIRLARGGAKKHPFYRVVIADARMPRDGRFIEKIGTFDPVK
                     PKDDAKRLVLDVEKAKAWLAKGAQPTDRVLRLLDDLGLAKRPARNNPVKAQPKKKAQE
                     RAAAAAKAAEAAAGAEAPAA"
     gene            complement(2618..4201)
                     /gene="ffh"
                     /locus_tag="VKU03_02780"
     CDS             complement(2618..4201)
                     /gene="ffh"
                     /locus_tag="VKU03_02780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0048501 - signal recognition particle,
                     plasma membrane targeting [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005048 - signal sequence binding
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle protein"
                     /protein_id="HLJ70215.1"
                     /translation="MFESLSEKLSGIFDKLTGRGALSEADVTAAMREVRRALLEADVA
                     LDVVRSFTDRARTKAVGAQVLKSVTPGQMVVKIVHDELVAMLGGEGEPIDLAAAAPVP
                     IMLVGLQGAGKTTSAAKIAKRLTERDKRKVLMASLDVKRPAAQEQLAVLGRQVGVDTL
                     AIVPGQQPVEIAKRAEAAARLQGYDVVILDTAGRTHIDEPLMAEMAEIKAAAKPHEIL
                     LVADALTGQDAVNLAKNFNERVGITGIVLTRADGDGRGGAALSMRAVTGKPIKLLGIG
                     EKMDAIEDFYPRRLADRILGMGDIVSLVEKAAQSIDAAQAAKMAEKMRKGVFDLDDLS
                     EQLAQIEKIGGMGGIMSMLPGMGKMKEQMAAAHLDEKIIARQRAIISSMTPRERRNPD
                     LLKASRKKRIASGSGTRVEDVNRLLKQHRQMADMMKAMGQAAGGKRGPMAKLGQMLGL
                     GGGMPMPTQEQIEAMRKEMGGKLPGAPGAGAGGGIPEAPPPGLFNNPPAFPGLGGLGA
                     PRPPGLGGAPFNPFGGKKK"
     gene            complement(4445..>5646)
                     /locus_tag="VKU03_02785"
     CDS             complement(4445..>5646)
                     /locus_tag="VKU03_02785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLJ70216.1"
                     /translation="ELEIAQRKGELGRAGELAYGVIPDLEKKLKEAEGHEVAGGKGVK
                     EEVTPEDIAAVVSRWTGVPVDKMLSGEREKLLKMEDELAKRVVGQSEAVAAVATAVRR
                     ARAGLQDPNRPIGSFMFLGPTGVGKTELTKALAAFLFDDEQAMVRIDMSEYMEKHSVS
                     RLIGAPPGYVGYDEGGALTEAVRRRPYQVVLFDEIEKAHPDVFNVLLQVLDDGRLTDG
                     QGRTVDFRNVLIVMTSNLGAEYLVAQKEGEDSSAVEGEVMAVVRSHFRPEFLNRVDEI
                     ILFHRLRREDMGAIVDIQFKRIAKLLEDRKIVLQLDAKGRQWLAEKGYDPAYGARPLK
                     RVIQKSVQDPLAERLLAGKIKDGETVKISAAGGALTIGDEPVVSRSEEEPSRAKVGTI
                     VNFPKGA"
BASE COUNT          834 a         1961 c         1867 g          984 t
ORIGIN      
        1 ggcggacgga tccagctcac ccgcggttcg aagccgaggg gcatacgatc ccgtccgccc
       61 ggccttccgg gggcggccgc ctccggaagg ccgacccgca tatcaatccg attcgggtcg
      121 tcttttcatg caaggggcag cgccgaacgt tgtgactagc gtctcgtagg gtgcgtacct
      181 ttccagtcct gcgcagcccc tcatccggcg cttcgcgcca ccttctcccc gcttcgcggg
      241 gagaagggat ttcccggccg ccttctcagt ctttcagcaa atccggccgc cgcgctttcg
      301 tcagcttctc cgcctgctcg cgccgccatt cggcgatgcg ggcgtggtcg ccgttgagca
      361 gcacgtccgg tatcgccctg ccctcccatt cgcgcggccg cgtatagtgc gggtattcga
      421 gcaggccgga ttcgaagctc tcttccgcgc cggattccgc cttgcccatc acgccgggga
      481 tcagccgcac gcaggcgtcg agcagcgcca gcgcggcgat ctcgccgccg gaaagaatgt
      541 agtcgccgat cgagacttcg atgaggttgc gcgcttcgat cacgcgctcg tcgacgccct
      601 cgaagcgcgg acagacgatg acgaggcccg gccccgcggc ccattgcttg acgcgggcct
      661 gactgaggcg ctcgccgcgc ggcgacatga tcaggcgcgg gcgcggatcg tctggttgca
      721 acgccgcgtc caggctcgcc gccagcacgt cggcgcgcat caccatccct ggtccgccgc
      781 cggccggtgt gtcgtcgacc gcgcgatggc ggccgatccc gtgcgcgcgg atgttcagcg
      841 tctcgagcga ccagaggccg cgctggagcg cgtcgccggc gagcgatagg ccgagcggcc
      901 cggggaacat ttcggggaag agggtcagga gggtggcgga gaacatggcg cgatcctagg
      961 cggcgtttat gcggcgcgag gtaaaatggc gaacgccgcg gcgccacgcc ggccgagcgc
     1021 cgtcaacccg ccatagcgcc acgacggctc gtgcaaaggt acatccacct gcaaggcgct
     1081 atggcgggtg ctccgcttcg ctgcgcccct tcgggtgact tcaatcgtcc ggcgcggcat
     1141 tgtggcgact gagaactttg gtgacgggaa gcggcccttg gccggcttcc cgtcgccaaa
     1201 gttctcagga gcaactgcgc catcgccgag cggaggagtc aaggttcgcg cagcgaagcg
     1261 gagcgaccgg cgaagccggc tcgaccttga cgccgagcac ggcgatggca tgcaggatga
     1321 agcgcgggaa ccgccgagcc ttacgactgg attgcttcgt cgctacgctc ctcgcaatga
     1381 cggtcaatcc tcgtgaaggc gccgctcgcc tcctccctca atttcccgcg gcggcgcgat
     1441 cacgatccgc ccgccggcga tgtccacctc cggcgcgacc gccttggtga acggaaacag
     1501 cagcgtctcg ccgccgcctt tcggcgcgac ttcgaggatg tcgcccgcgc cgtaattgtt
     1561 cagcgcgacg acgcggccga tctcttcgcc cgccgccgtc tcggcgcgca ggccgacgag
     1621 atcggcgtgg tagaattcat cctcgtccgg cggcggcatg ttctcgcgtc gcgcgaagag
     1681 ttcggttccg gtcaacgcgg ccgcggcgcc gcggtcgccg acgccgtcga ggcgtacgac
     1741 gaccatatcg tccttcagcg gccgcagcgc gcttatcttg aagcgccgcg cgccggcgac
     1801 atcggtcagc tcgccatagg cggcgagcga cttgggatcg gcggtatagg ccttgacccg
     1861 cacctcgccg cgcacgccat gcggcgcgcc gaacattccg acggccacca ggcgcggcgg
     1921 agccatgcgc gactaggccg ccggcgcttc cgctccggcg gcggcttcgg ccgccttggc
     1981 cgcggcggcg gcgcgctctt gcgccttctt cttaggctgc gccttgaccg gattgttgcg
     2041 cgccggccgt ttggcgagac cgagatcgtc gagcaggcgc aggacgcggt cggtcggctg
     2101 ggcgcccttg gccagccagg ccttggcctt ctcgacatcg aggacgagac gcttggcgtc
     2161 gtccttcggc ttgaccggat cgaacgtgcc gatcttctcg atgaagcggc cgtcgcgcgg
     2221 catacgcgca tcggcgatga cgacgcggta gaacggatgc ttcttggcgc cgccgcgggc
     2281 gaggcggatt ttcagggaca tggttcgtac tttcctgctt tcttgatgga tcaattctgc
     2341 tggttgacga tgcgccgcgc gcgcagactt cgcgcggcga tgaagttgta cgaagcaatt
     2401 gcgcgtttga tattcgctcg aggcgccgag ccatgagcgc aatccgtcat tgtttggaac
     2461 gctggcccga atgcgccggg aaaagaggct gtcaagggtc tacgaccgcg aagcggcgct
     2521 tcgcgccctt gacagcctct tttcccggcg cgcctgctcg gcgttccaaa caatgacgga
     2581 ttgcgctcat ccgccccgtt tggggatgaa gggaagctca cttcttcttc cccccgaacg
     2641 gattgaacgg cgcgccgccg agtccgggcg gccgcggggc gccgagtccg ccgagccccg
     2701 ggaaggccgg cggattgttg aacaatccgg gcggcggcgc ctcgggaatg ccgccgcctg
     2761 cgcctgcgcc cggcgcgccc ggcagcttgc cgcccatctc cttgcgcatc gcctcgatct
     2821 gctcttgcgt cggcatcggc atgccgccgc caaggccgag catctggccg agcttggcca
     2881 tcggccctcg cttgccgccc gccgcctggc ccatcgcctt catcatgtcg gccatctggc
     2941 gatgctgctt caacagccgg ttgacatcct cgactctggt gccagagccc gaagcgatgc
     3001 gcttcttgcg cgaggctttc agcaagtccg gattgcgccg ctccctcggc gtcatcgacg
     3061 aaatgatcgc gcgctggcgg gcgatgatct tctcgtcgag atgcgcggcc gccatctgct
     3121 ccttcatctt gcccatgccg ggcagcatgc tcatgatgcc gcccatgccg ccgatcttct
     3181 cgatctgggc gagctgctcg gaaagatcgt cgaggtcgaa gacgcccttg cgcatcttct
     3241 cggccatctt ggccgcctgc gccgcgtcga tcgactgcgc cgccttttcg accagcgaga
     3301 cgatgtcgcc catgccgaga atgcggtcgg cgagacggcg cggatagaaa tcctcgatcg
     3361 cgtccatctt ctcgccgatt ccgagcagct tgatcggctt gccggtgacg gcgcgcatcg
     3421 agagcgccgc gccgccgcgg ccgtcgccgt cggcgcgggt gaggacgatg ccggtaatcc
     3481 cgacgcgctc gttgaaattc ttggcgaggt tgaccgcgtc ctggccggtc agcgcgtcgg
     3541 cgacgagcag aatctcatgc ggcttggcgg ccgccttgat ctcggccatc tcggccatca
     3601 agggctcgtc gatatgggtg cggccggccg tgtcgaggat gaccacgtcg taaccttgca
     3661 gccgcgcggc ggcctcggcg cgcttggcga tctcgaccgg ctgctggccg ggcacgatgg
     3721 cgagcgtatc gacgccgacc tggcggccga gcacggcgag ctgctcctgc gccgccggcc
     3781 gcttcacatc gagcgaggcc atcagcactt tgcgcttgtc gcgctcggtc aggcgcttgg
     3841 cgatcttggc ggcgctcgtc gtcttgccgg cgccttgcaa gccgaccagc atgatcggaa
     3901 ccggagccgc cgccgccagg tcgatcggct cgccctcgcc gcccagcatc gcgacaagct
     3961 cgtcatggac gatcttgacg accatctggc cgggcgtgac cgacttcaac acctgcgcgc
     4021 cgaccgcctt ggtgcgggcg cggtcggtga aggagcgcac cacgtcgagc gcgacgtcgg
     4081 cttcgagcaa ggcgcggcgc acctcgcgca tcgcggcggt cacatccgcc tccgacaacg
     4141 cgccgcggcc ggtcagctta tcgaaaatgc cggagagctt ctcggaaagg ctctcgaaca
     4201 tggggcgctc ctaaaggccc gccagcgcca atcgcgcccg ggggcgcatc gcgctgccgg
     4261 gcggggacct ccagcctcga agggctggtc ggcttaatcg acgaggacgg gtcctgacga
     4321 aatttgagga ggaaataggg ggaaggaggc gggatgtcaa atgagcgccg ccccaccgat
     4381 gaggtacagg ctcccggctc cccgcttacg gggagaagcg gggagagggc ttcaatcgcg
     4441 atcgtcacgc gcctttcggg aagttgacga tcgtgccgac tttggcgcgg gagggctcct
     4501 cctcgcttcg gctgacgacc ggctcgtcgc cgatcgtcag cgcgccgccg gcggcggaga
     4561 ttttcaccgt ctcgccgtcc ttgatcttgc cggcgagcag ccgctcggcg agcggatcct
     4621 gcaccgattt ctggatcacc cgcttcaacg gccgcgcgcc ataggccgga tcgtagccct
     4681 tctccgccag ccattgccgg cccttggcgt cgagctgcag gacaatcttg cgatcctcga
     4741 gcagcttggc gatccgcttg aactggatat cgacgatcgc gcccatgtcc tcgcggcgca
     4801 agcgatggaa caggatgatc tcgtcgacgc ggttcaagaa ttccggccgg aaatggctgc
     4861 gcaccaccgc catcacctcg ccctcgaccg cgctcgaatc ctcgccctcc ttctgggcga
     4921 cgagatattc ggcgccgaga ttcgatgtca tgacgatcag cacattgcgg aaatcgactg
     4981 tgcggccctg gccgtcggtc aggcggccgt cgtcgagcac ttgcaagagg acgttgaaca
     5041 cgtccggatg cgccttctcg atctcgtcga acagcacgac ttggtacggc cggcggcgca
     5101 ccgcctcggt cagcgccccg ccctcgtcat agccgacata gccgggcggc gcgccgatca
     5161 ggcgcgagac cgaatgcttc tccatatatt cggacatgtc gatgcgcacc atcgcctgct
     5221 cgtcgtcgaa taggaacgcg gcgagcgcct tggtgagctc ggtcttgccg acgccggtcg
     5281 gccccaagaa catgaacgag ccgatcggcc ggttcgggtc ctgcaggccc gcgcgcgccc
     5341 ggcgcacggc ggtggcgacg gccgcgaccg cttcggactg gccgacgacg cgcttggcga
     5401 gctcgtcctc catcttgagg agcttctcgc gctcgcccga cagcatcttg tcgaccggca
     5461 cgccggtcca gcgcgagacg acggccgcga tgtcctcggg cgtgacctct tccttcacgc
     5521 ccttgccgcc ggccacctcg tggccttcgg cctccttgag cttcttctcg aggtcgggga
     5581 tcacgccata ggccagctcg cccgcccgac cgagctcgcc cttgcgctgg gcgatctcga
     5641 gctcgg
//
LOCUS       DATGJW010000093         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1261289, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000093 DATGJW010000000
VERSION     DATGJW010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1261289"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..466
                     /locus_tag="VKU03_02790"
     CDS             <1..466
                     /locus_tag="VKU03_02790"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLJ70217.1"
                     /translation="LSSQTMHVASSAGESYDWPISSGRNGHLTPRGSFRPIALYPMVH
                     SAKYDNAPMPHSIFFLSQYAIHGTNAVGSLGRPASHGCIRLAPGHAAALYEMVQAEGA
                     SIRITGDAPVGRIQVAERRHRHVATALGYAPWRRAPTLREWARNPGWIPLEQ"
     gene            547..1839
                     /locus_tag="VKU03_02795"
     CDS             547..1839
                     /locus_tag="VKU03_02795"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HLJ70218.1"
                     /translation="MNSARSGALAREDVGAAMRAIGAEARQASRVLANAPREQKDHAL
                     IAAAAALRAHAAQILAANARDIAAAKARGLAASFIDRLTLDDKRIEAMARGLEEIAAL
                     PDPVGRVLAHYERPNGLLIERVATPLGVVGVIYESRPNVTADAGALCLKSGNAVVLRG
                     GSDSFHSSTAIHACLVEGLAAAGLPEAVIAMPASASREAVAEMLAGLDGALDVIVPRG
                     GKSLVARVQNEARVPVFAHLEGVVHVYVDSAADLEVAKKVLINSKLRRTGICGAAEKL
                     LVDKTCAATHLKPLVQALLDAGCAVRGDAAALAADPRVTKASEEDWRAEYLDAIITAK
                     VIDGLDAAIDHIETYGSHHTDCILTEDKAAAERFLKEVDSAIVLHNASTQFADGGEFG
                     FGGEIGIATGRMHARGPVGLEQLCTFKYRVHGDGQVRG"
     gene            complement(1933..2433)
                     /locus_tag="VKU03_02800"
     CDS             complement(1933..2433)
                     /locus_tag="VKU03_02800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLJ70219.1"
                     /translation="MPESVIDDFDRALLDLVQSDNLTPARVLAERVGLSESAVLRRLR
                     RLRREKIITADVSIVRPAAIGLPLTIVALVSLERENGGVLDDFARRAKARPEVRQCWY
                     VTGESDLALVLQLESMASYEAFTRDMFIDNLNVRSFKTLVAMREIVNETEARKPLAAR
                     KAKKNR"
     gene            2519..>3170
                     /locus_tag="VKU03_02805"
     CDS             2519..>3170
                     /locus_tag="VKU03_02805"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLJ70220.1"
                     /translation="MSDRVFIMNADPESPAALVALCPAHAATPLLDLPRLAAALQVAS
                     LSVKDEGRRPLGSFKALGGVYAGLRALARAAGVPTRALLDPARVRTPLPALVCASAGN
                     HGLSVASAARLAGARARVFIAEAIPEARAARVADKGAEVVRVAGSFDDAVRRATEAAR
                     AQGDLLIPDTSADPDDPVVRDVMAGYGILAEEIRAEIAAGRRQAPTHIFVQAGVGGL"
BASE COUNT          530 a         1008 c         1060 g          572 t
ORIGIN      
        1 tctttcctcg cagacgatgc atgtcgcctc gagcgccggc gagagctacg attggccgat
       61 ttcctctgga cgcaacggcc atttgacgcc gcgcggctcg ttccggccaa tcgcgcttta
      121 tccaatggtt cattcggcga aatacgacaa tgcgccgatg ccgcattcga ttttcttcct
      181 cagccaatac gccatccatg ggacgaacgc ggtcggctcc ctcggccgcc cggcgtcgca
      241 tggctgcata agactagcgc cgggccatgc ggcggctctc tatgaaatgg tccaggccga
      301 aggggcgtcc atccggatca ccggcgatgc gccggtggga cgcattcagg tcgccgagcg
      361 ccgccatcgc catgtcgcca cagccttggg ctacgcgccc tggcgacgcg cgccgacgct
      421 cagggaatgg gcgcgcaatc ccggttggat tccgctggag cagtaggcct aagggcgcct
      481 tgcgcttggg cttgccgccg ccgatgtgct aaagccccgc caaggagctt atttgagaga
      541 acggcaatga attcggctcg ttccggcgcg cttgcgcgcg aggatgtcgg cgcggccatg
      601 cgggcgatcg gcgcggaagc gcggcaggca tcgcgtgttt tggcgaatgc gccgcgcgaa
      661 cagaaagatc acgcactgat cgcggctgcg gcggcgcttc gagcgcacgc cgcgcaaatc
      721 cttgccgcca acgcccgcga catcgccgcc gccaaggcgc gtggcctagc ggcctccttt
      781 atcgaccgcc tgaccctgga cgataagcgg atcgaggcca tggcgcgcgg cctcgaggag
      841 atcgcagcct tgcccgatcc ggtagggcgc gtcctggcgc attacgagcg gccgaatggc
      901 cttttgatcg aacgcgtggc gacgccgctt ggggtcgttg gcgtgattta tgaaagccgg
      961 ccgaacgtta cggccgacgc cggcgcgctg tgccttaagt cgggcaacgc tgtcgtgctg
     1021 cgcggcggtt cggatagttt tcattcctct acggcgattc atgcctgcct ggtcgaaggc
     1081 ctcgcggctg ccggcctgcc cgaggccgtg atcgcgatgc cggcctcggc ctcgcgcgag
     1141 gctgtcgctg aaatgctcgc agggcttgac ggcgcgctcg acgtgatcgt gccgcggggc
     1201 ggcaaaagcc tggtcgcgcg ggtgcagaac gaggcgcgcg tgccggtttt cgcccatctc
     1261 gaaggcgtgg tgcacgtcta tgtcgatagc gccgccgatc ttgaagtggc gaagaaggtt
     1321 ctcatcaatt cgaagctgcg acgtaccggc atttgcggcg cggcggagaa gctgctcgtc
     1381 gataagactt gcgcggcgac gcatctgaag ccgctcgtcc aagccttgct tgacgccggc
     1441 tgcgcggtgc gcggcgacgc cgccgctttg gcggccgacc cgcgcgtgac caaagcgagc
     1501 gaagaggatt ggcgcgccga atatctcgac gccattatca cagcgaaagt catcgacgga
     1561 ctggacgcgg cgatcgatca catcgagaca tatggctcgc atcacaccga ctgtatcttg
     1621 accgaggaca aggcggccgc cgaacggttt ttgaaagaag tcgattccgc gatcgtcctg
     1681 cataatgctt cgacgcaatt cgccgacggc ggcgaattcg gtttcggcgg cgaaatcggc
     1741 atcgcgaccg ggcgcatgca tgcccgcggg cctgtggggc tggagcagct ttgtacgttc
     1801 aaatacagag tgcatggcga cgggcaggtg cgggggtagg tggtctcagc gagcaaagcg
     1861 aagcaatcca gaagcctcag aggtttcggg attgcttcgt cgcttcgctc ctcgcaacga
     1921 cggaacgtga ctctatctgt tcttttttgc tttccgcgcc gccagcggct ttcgcgcctc
     1981 tgtttcgttc acgatctcgc gcatcgccac cagcgtcttg aacgatcgca cgttcagatt
     2041 gtcgatgaac atatcgcggg tgaaagcctc gtagctcgcc atcgactcga gctgcagcac
     2101 tagcgccaga tcggactcgc cggtcacgta ccaacattgg cgcacctcag gcctagcctt
     2161 cgcacggcgg gcgaaatcgt cgaggacgcc gccgttctcg cgttcgagcg agacgagcgc
     2221 gacgatcgtc aggggcaagc cgatcgcggc gggacgtacg atcgaaacgt cggcggtgat
     2281 gattttctcg cggcgcagcc ggcggaggcg gcgcaggacg gcgctctcgg aaaggccgac
     2341 ccgctccgcc aagacgcgcg ccggggttag attatcgctc tggacgaggt ccagtagagc
     2401 gcggtcgaaa tcgtcgatga cggattcagg catgagagcg acaaactgcg ccagaaacag
     2461 tcggccgagc aactgatttc gccgacacgc gtcgcgccgg tcggcttatg gtttgccgat
     2521 gtccgataga gttttcatca tgaatgccga tcccgaaagc cccgccgccc ttgtggcgct
     2581 ttgtcccgcg catgcggcga cgccgctgct cgatctgccg aggctcgccg cggcgctgca
     2641 ggtcgcaagc ctttccgtta aggacgaagg gcgccggccg cttggcagtt tcaaagccct
     2701 gggcggcgtt tacgctgggc tccgcgcgct ggcccgcgcc gccggcgttc caacgcgggc
     2761 gctgctggat ccggcgcgcg ttcggacgcc gcttccagcg cttgtctgcg ccagcgctgg
     2821 caatcatggc ctttcggtcg cttccgcggc gcgtctcgcc ggcgcgaggg cgcgcgtctt
     2881 catcgcagag gcaatccccg aggcgcgcgc ggcccgcgtc gccgacaagg gcgcggaagt
     2941 cgtgcgtgtc gcgggctcat tcgacgacgc cgtgcgacgg gccacggaag ctgcgcgggc
     3001 gcaaggagat cttctgattc ccgatacaag cgccgacccg gacgatccgg tcgttcgtga
     3061 cgtgatggcg ggatacggaa tccttgccga ggagatacgc gccgagatcg ccgccggacg
     3121 gcggcaagca ccgacccata ttttcgtgca agcgggcgtc ggtggcctcg
//
LOCUS       DATGJW010000094         2403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1156514, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000094 DATGJW010000000
VERSION     DATGJW010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2403
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1156514"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1033
                     /locus_tag="VKU03_02810"
     CDS             116..1033
                     /locus_tag="VKU03_02810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966055.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar kinase"
                     /protein_id="HLJ70221.1"
                     /translation="MSLDIVGMGEPMLELNQVKGGDGYRPGFGGDTANTIVAAARSGA
                     KTGYFTGLGEDTFGRAFLDLWQSEGVDASQVKIDADAHTGVYFVTHGPQGHEFSYLRA
                     GSAASRLTEADIPSDYVASARILHVSGISQAISSSAADAVFAAIDLAKSSKALVSYDT
                     NLRLKLWPLTRARAVIHAAMRLADIALPGLDDARLLTGLEAPDAIADFYLRLGAKIVA
                     LTLGPEGALVATEKRRETLASLKVEAVDATGAGDTFDGAFLAEYLRTGDPFAAGRFAV
                     VAAALSTRGYGAIAPMPRREETLAAMAKM"
     gene            1376..2224
                     /locus_tag="VKU03_02815"
     CDS             1376..2224
                     /locus_tag="VKU03_02815"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HLJ70222.1"
                     /translation="MADQAFNSSDAAAARELLVEAARAAGRLALACFKPGERTAAHIR
                     LKGGGSPVTEADLAVDEFLKGEFARLFPKAGWLSEETEDDASRLGRAQILVVDPIDGT
                     RAFIDGDPRWTVSIALVVAGRPAAGVVHAPALDETYAASAGGGASLNGRAIAVSARGG
                     LDGAHIGGPRGMVEALERGAGLALRLEPKIPSLAYRLALVARGALDLAIASDKSHDWD
                     IAAADLILAEAGGSLVDARGQGLLYNREDTLHPALFAAPRKLIGQALAAAASARTGRS
                     SLASPA"
     gene            2256..>2403
                     /locus_tag="VKU03_02820"
     CDS             2256..>2403
                     /locus_tag="VKU03_02820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HLJ70223.1"
                     /translation="MTEPGRDEVASAESPQLLHLVFGGELKNLETYEFRDPSKIDVVG
                     IFPDY"
BASE COUNT          381 a          816 c          798 g          408 t
ORIGIN      
        1 tgcgccttat cgagtgtcag gtcgctcatc ttggctcctt atttttattt tgcgagaaag
       61 agttgaccgg tccaggctct ctcgtcaatg tccggagcga acagcgggag tgcaaatgtc
      121 attggatatt gtcggcatgg gcgaacccat gctcgagctc aatcaagtca aaggcggtga
      181 cggctatagg cccggattcg gcggcgatac ggccaatacg attgtggcag cggcgcgttc
      241 gggtgcaaag acgggctatt tcacggggct tggcgaagac actttcggtc gcgcctttct
      301 cgatctgtgg caaagcgagg gagtcgatgc gtcgcaggtc aaaatcgacg cagacgccca
      361 taccggcgtc tatttcgtga cccatggccc gcaaggtcac gaattttcct atttgcgcgc
      421 cggctcggcg gcgagccgcc tcaccgaagc ggatattcct tccgactatg tcgcctcggc
      481 gcgaatcctt catgtctcgg ggatcagcca ggcgatctcc tcgtcggccg ccgacgcggt
      541 tttcgcggcg atcgacctcg caaaaagcag caaggcgctg gtctcgtacg atacgaattt
      601 gcgcttgaag ctgtggccgc tgaccagagc gcgcgccgtg attcacgcgg cgatgcggct
      661 tgccgacatc gccttgcccg ggctcgacga cgcgcgcctg ctgaccggcc tcgaagcgcc
      721 cgacgcgatc gccgatttct atctgcggct cggcgcgaaa atcgtcgcgc tgacgctcgg
      781 acccgagggc gcgctcgtcg cgacggaaaa gcggcgcgaa accctcgcct cgctcaaggt
      841 cgaggcggtc gatgcgaccg gcgccggcga cacgttcgac ggcgccttcc tcgccgaata
      901 tttaaggacc ggcgatccct tcgccgccgg ccgcttcgcc gtcgtcgccg ccgccctgtc
      961 gacgcgcggc tacggcgcca tcgcgccgat gccgcgccgc gaggagacgc tggcggcgat
     1021 ggctaaaatg taagcctcca ccctctcccg accattcggg actttccgcg agaaaagctc
     1081 gaagtccgta cgctcagacg tgtcgagagg ttctacaggc gcggcgacgt accctcatcc
     1141 gaccatttcg cggccatttg tcccgcaagc gcgagaaggg ttgtagccgc ggtcgccgac
     1201 cgcggctaca gcgcagcggc ggataaacgc cgcggcgcga gggtgagccg ccgcctcgac
     1261 gatggcggcg acaggccgcc cggtcggctg ctgcgcccgg tctccggagc ggaaaggttg
     1321 cgcggccgga acgccgccgc tagagcatcg gcgcaaacgc aaaggacgcc gtttcttggc
     1381 cgaccaggct ttcaattcca gcgacgcggc ggcggcgcgc gagcttctcg tcgaagcggc
     1441 gcgcgcggcc ggacgcctag ccctcgcctg tttcaagccg ggcgagcgga cggcggcgca
     1501 tattcgcctg aaaggcggcg gctcgccggt gactgaggcc gacctcgcgg tagacgaatt
     1561 cctgaagggc gagttcgccc gccttttccc caaagcggga tggctttccg aggaaacgga
     1621 ggacgacgcc tcccggctgg gccgcgcgca aatcctcgtc gtcgatccga tcgacggcac
     1681 gcgcgccttc atcgacggcg atccgcgctg gaccgtctcg atcgcgctgg tcgtcgccgg
     1741 ccggccggcc gcgggggtcg tccacgcgcc ggcgctcgat gaaacctatg ccgcttccgc
     1801 cggcggcggc gcctcgctca atggccgcgc catcgcggtc tcggcgcgcg gcgggctcga
     1861 cggcgcccat atcggcggcc cgcgcggcat ggtcgaggcg cttgagcgcg gcgccggctt
     1921 ggcgctgcgg cttgagccga aaatcccgtc gctcgcctat cgcctggcgc tcgtcgcgcg
     1981 tggcgcgctc gatctcgcca tcgcctccga caagtcgcac gattgggaca tcgccgcggc
     2041 cgatctcatt ctcgccgagg cgggcgggtc gctggtcgat gcgcgcgggc aaggcctcct
     2101 ctacaatcga gaggacacgc tgcatccggc gctgttcgca gcgccgcgca agctgatcgg
     2161 gcaggcgctg gcggcggccg cttcggcccg gacggggcgc tcgagcctgg cctcccccgc
     2221 ctgagccgcg ccgcccaccc ttcaaggagc ctgtcatgac ggaacccgga cgagacgagg
     2281 tcgcgagcgc ggagtcgccg cagctcctgc atcttgtctt cggcggcgag ctgaaaaatc
     2341 tcgaaacgta cgaatttcgc gatccgtcga aaatcgacgt ggtcggcatc tttcccgact
     2401 acg
//
LOCUS       DATGJW010000095         4309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_846529, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000095 DATGJW010000000
VERSION     DATGJW010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4309
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846529"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            283..661
                     /gene="rnpB"
                     /locus_tag="VKU03_02825"
     ncRNA           283..661
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VKU03_02825"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            complement(766..2025)
                     /locus_tag="VKU03_02830"
     CDS             complement(766..2025)
                     /locus_tag="VKU03_02830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590401.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLJ70224.1"
                     /translation="MNDAVVSTRCCIAGGGPAGMMAGFLLARAGIDVIVLEKHEDFLR
                     DFRGDTIHPSTLNLMRELGFLDEFLELPHQKVFQLEGWIGDERAPLADFSALPPPCNY
                     IAMMPQWDFLNFLAAKAAALPHFKLMMRTEARDLVIERDKVVGLSARSPDGPLTIRAD
                     LVIAADGRHSVLPDQVGLKTIDRGAPMDVLWFRVPRRDGDDDQTIGRFVNGRILIRIQ
                     RGAYWQCGYVIAKGAFEDVRAEGIEAFRNSVAELMALPRQRLEALESFDDVKLLSVSV
                     DRLERWRRPGFLAIGDAAHAMSPVGGVGVNLAVQDAVAAANLLAAPLLARNLTEDHLA
                     AVERRRLFAVRVIQAGQVAIQKRIIGRVLAANRPIRPPFFFRIVARSAALRSLLARII
                     GIGVRPEHIQTAPAFPPPSKTGEGDRA"
     gene            2387..2938
                     /locus_tag="VKU03_02835"
     CDS             2387..2938
                     /locus_tag="VKU03_02835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009735140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreE"
                     /protein_id="HLJ70225.1"
                     /translation="MLAARSVRPAGSWTGAPADSIVLDYDERHRRRVTMAGVRGLAFL
                     LDLAEATMLRGGDGLALDDGRIVEVVAAPEPLAEIRCKDALALARIAWHLGNRHLPVE
                     LTRRALRIRRDHVIEEMARGLGGEVAAIEAPFNPEGGAYAASHAHEHGHDADHHGHGH
                     DHHDHGHERRPHAHDHRHERGAG"
     gene            2838..3620
                     /locus_tag="VKU03_02840"
     CDS             2838..3620
                     /locus_tag="VKU03_02840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016151 - nickel cation binding [Evidence
                     IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory UreF family protein"
                     /protein_id="HLJ70226.1"
                     /translation="MTPTITVTVTIITITATSAGLTRTIIAMSAARAEAESFPELPLL
                     LWLSPAFPVGSFAYSHGLEWAVEAGDIADAASLRGWLGDLLSFGAPRNDAILFASAFR
                     AAAAEDKKGIARLNELAVALTGSAERRLETVAQGGAFLAAMRGAWATPALDLIDAESD
                     EPVAYAIAVALAASGHGLALATSLEAFVLSLLANLVSAAVRLGPIGQSDGQRVLAGLL
                     PEVRRLAAEAADATPDDLGGCALRSDIAAMRHETQYSRLFRS"
     gene            3617..4234
                     /gene="ureG"
                     /locus_tag="VKU03_02845"
     CDS             3617..4234
                     /gene="ureG"
                     /locus_tag="VKU03_02845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019627 - urea metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urease accessory protein UreG"
                     /protein_id="HLJ70227.1"
                     /translation="MSRHDQPLRVGIGGPVGSGKTALMEQLCKALRGRYEIAAITNDI
                     YTKEDARILVEAGALEPERIMGVETGGCPHTAIREDASINLAAIEAMRRRFPRLDLIL
                     VESGGDNLAATFSPELADLTIYVIDVSGGEKIPRKGGPGVTRSDLLVINKIDLAPYVG
                     ASLDVMARDAKAMRKERPFVLANMKTGEGLSAVIDFIERKGGLAD"
BASE COUNT          719 a         1467 c         1460 g          663 t
ORIGIN      
        1 gacgccgccc cgcggcgatg gcagcgcggt gtaggggatt tgcgtcatcg ttgcatccgt
       61 actgctcgca agagtctgtg agcagccccg aggtccgcat gtttattttc tgagtctcgg
      121 cgccggaaac tcaacgagga atctcgaaat cgcgttattt cacgagactt cgtcgacgac
      181 gatgcgactc gcctctcact gacagccctg cgccttgccg ggagaagaga tttcccccgc
      241 cccggcttga ccgctctctc tcgccctctt atctcctccc cgccagtcgg ccggacggcc
      301 gccgcgtcag cggaggaaag tccgggctcc acggaaatac ggcgccggat aacgtccggc
      361 gggggcgacc ccagggatag cgccacagaa agcaaaccgc cggcttggcc ggcaagggtg
      421 aaagggtgcg gcaagagcgc accgcgcggg cggcaacgtt ccgcggcatg gcaagccccg
      481 ccgggagcaa aaccgaatag gagcgatgag gaggttcgcc tccgcgccag tttccgggcg
      541 ccgtcgctcg ggttggttgc ttgaggcgcc cggcgacggg cgtcccagag gaatggccgt
      601 cgcgcgcggc gcttcacggc gccgcgccct acagaacccg gcttacaggc cgactggcta
      661 ttatcctccc tcgcgaagcg gggcaggggg accatgcgaa tgcatggtcg agggggtctg
      721 cggcttttga ggatagctcc agactcagcg gccccctcca ccgcgctacg cgcggtcccc
      781 ctcccccgtc ttcgacgggg gaggaaaggc cggcgccgtc tgaatatgct ccggcctgac
      841 gccgatcccg atgatccggg caagcagcga gcgcaaagcg gccgagcgcg cgacgatgcg
      901 gaagaagaac ggtggcctga tcggccggtt ggcggcgagc acgcggccga tgatccgctt
      961 ttggatcgcg acctgcccgg cctggatgac ccgcaccgcg aagagccgcc gccgctcgac
     1021 cgccgccaaa tgatcttccg tgagattgcg cgcaagcagc ggcgcggcga gcaaattggc
     1081 cgccgccacc gcgtcctgca ccgcgagatt gacgccgacg ccgccgaccg ggctcatcgc
     1141 atgggcggcg tcgccgatgg cgagaaagcc cggccggcgc cagcgctcca gccggtcgac
     1201 cgagacgctg agcagcttga cgtcgtcgaa ggattcgagc gcctccagcc gctggcgcgg
     1261 cagcgccatc aattcggcga cgctgttgcg gaaagcctcg atcccctcgg cgcgcacgtc
     1321 ctcgaacgcg cctttggcga tgacgtagcc gcattgccaa taggcgccgc gctggatgcg
     1381 gatgagaatc cggccattga cgaagcggcc gatcgtctgg tcatcgtcgc cgtcgcggcg
     1441 cggaacgcgg aaccacagaa cgtccatggg cgcgccgcgg tcgatcgtct tgaggccgac
     1501 ctggtcgggc agcaccgaat ggcggccatc ggcggcaatg acgagatcgg cgcggatcgt
     1561 cagcgggccg tcgggcgagc gcgccgaaag gccgacgacc ttgtcgcgct cgatgaccag
     1621 gtcgcgcgcc tccgtccgca tcatcagctt gaaatgcggc agcgccgccg ccttggcggc
     1681 gaggaaattg aggaagtccc attgcggcat catggcgatg tagttgcagg gcggcggaag
     1741 ggcggaaaaa tcagcgagcg gagcgcgctc gtcgccgatc cagccttcga gctggaacac
     1801 tttctgatgc ggcaattcga ggaactcgtc gagaaaaccg agttcgcgca tcagattgag
     1861 ggtggaggga tggatcgtgt cgccgcggaa gtcgcgcaga aagtcctcgt gcttttcgag
     1921 cacgatcacg tcgattccgg cccgcgccag caggaagccc gccatcatgc ccgccgggcc
     1981 gccgccggcg atgcaacagc gcgtcgaaac aacggcgtcg ttcatggcgg cccctcaagc
     2041 gctgcccgat ggcgcatcta gcggagattt ggggatttgc gcaaactttt ggcctgcgaa
     2101 agcgcgcgag ggctgcgtcg agtcggcggg tgagccaaga ggcattgcga gcggagcgaa
     2161 gcgaagcaat caaaggacag caagccccct cgcccccgcg cggcccacca gaacgagcat
     2221 gcgtggcctg ggccgcggcg caagcccttt gcgaatagta cggactgaac gaggcgaatc
     2281 cctgaacgcg gcgccgcggc ggcgacaaga gattggcgct tcttcccatc gcgccgcaag
     2341 ccggataaga aagcggcaga cgcccccgga tcttgcgaaa acgcccatgc tcgccgcccg
     2401 ctccgttcgc cccgccggct cctggaccgg cgcgccggcc gattccatcg tgctcgacta
     2461 cgacgagcgc catcgccggc gcgtgacgat ggccggcgtg cgcggccttg cttttctgct
     2521 cgaccttgcc gaagcgacga tgctgcgcgg cggcgacggc ctggcgctcg acgacggacg
     2581 catcgtcgaa gtcgtcgccg cgcccgagcc gctcgccgag attaggtgca aggacgcgct
     2641 cgcgcttgcc cgcatcgcct ggcacctcgg caatcgccat ctgccggtcg agctgacgcg
     2701 ccgcgcgctg cgcatcaggc gcgatcacgt catcgaggag atggcgcgcg gcctcggcgg
     2761 cgaagtcgcc gccatcgaag cgccgttcaa tccggaaggc ggggcctatg ccgcgtcgca
     2821 tgctcacgag catggtcatg acgccgacca tcacggtcac ggtcacgatc atcacgatca
     2881 cggccacgag cgccggcctc acgcgcacga tcatcgccat gagcgcggcg cgggctgagg
     2941 ccgaatcctt cccggagctc ccgctgctgc tctggctgtc gcccgccttt ccggtcggct
     3001 cgttcgccta ttcgcacggc ctggaatggg cggtcgaggc gggcgacatc gccgacgccg
     3061 cgagcttgcg cggctggctc ggcgatctcc tcagcttcgg cgcgccgcgc aacgacgcca
     3121 tactcttcgc ttcggcgttt cgcgccgcag cggctgagga taaaaagggg atcgcgcgct
     3181 tgaacgagct cgcggtcgcc ctgaccggct cggccgagcg ccggctggag acggtcgcgc
     3241 agggcggcgc cttcctcgcg gcgatgcgcg gcgcctgggc gacgccggcg ctcgatctca
     3301 tcgacgccga gagcgacgag cctgtcgcct atgcgatcgc cgtcgcgctg gcggcgtcgg
     3361 gccatggctt ggcgctcgca acctcgctgg aggctttcgt actttccctt ctcgccaact
     3421 tggtttctgc cgcggtgcgg ctcgggccga tcggccagag cgacggccag cgcgtcttgg
     3481 ccgggctcct gcccgaggtc aggcgcctgg cggcggaagc cgccgacgcg acgccggacg
     3541 atctcggcgg ctgcgccttg cgctcggaca tcgccgcgat gcggcacgaa acgcaatatt
     3601 cgaggctgtt ccgctcatga gccgtcacga ccaacctttg cgcgtcggca ttggcggccc
     3661 ggtcggctcc ggcaagaccg ccttgatgga gcaattgtgc aaggctctgc gcggccgcta
     3721 tgagatcgcc gcgatcacca acgacatcta tacgaaagag gacgcgcgca ttctcgtcga
     3781 ggccggcgcg ctcgagccgg agcggatcat gggcgtcgag accggcggct gcccgcacac
     3841 cgcgatccgc gaggacgcct cgatcaatct cgcggcgatc gaggcgatgc gccgccgctt
     3901 tccgcggctc gatctcatct tggtggaatc cggcggcgac aatctcgccg cgaccttctc
     3961 gccggagctc gccgacctga cgatctatgt gatcgatgtc tccggcggcg agaaaatccc
     4021 gcgcaaaggc gggccgggcg tcacccgctc cgatctcctc gtcatcaaca agatcgatct
     4081 tgcgccctat gtcggcgcct cgctcgacgt gatggcgcgc gacgccaagg cgatgcgcaa
     4141 ggagcgcccg tttgtcctcg ccaatatgaa aaccggcgaa ggcttaagcg cggtgatcga
     4201 cttcatcgag cgaaaaggcg gcctcgccga ctaggccgtc atggcgagcg gagcgaggcc
     4261 gtccgctcat ggagggccgc ccttaaggat ggatcgcgtc gtcgccgcg
//
LOCUS       DATGJW010000096         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_846776, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000096 DATGJW010000000
VERSION     DATGJW010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_846776"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            248..610
                     /locus_tag="VKU03_02850"
     CDS             248..610
                     /locus_tag="VKU03_02850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall anchor protein"
                     /protein_id="HLJ70228.1"
                     /translation="MTRSFSRAAALAAALVLSIILAGCNLATLDAKIAADASASLPTI
                     CSLGASAHAAFSTIAGTGQVSKAAAADEAAAYAGLQSLCANPPRNVSAAIVEAGSIYA
                     AIADSLSAAESASTPRGR"
     gene            621..986
                     /locus_tag="VKU03_02855"
     CDS             621..986
                     /locus_tag="VKU03_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70229.1"
                     /translation="MSVAFGSDTPWGLLEWVATGLSTAVLGSAAFAWRLAARLDTNSA
                     ALEWQKAELAAVKQAHDAAASRLADRLTQLLDDQCRLRETAAALPTRADLRDMEERLG
                     ERIQALAARIDGIVERGAA"
     gene            complement(983..1423)
                     /gene="ybgC"
                     /locus_tag="VKU03_02860"
     CDS             complement(983..1423)
                     /gene="ybgC"
                     /locus_tag="VKU03_02860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system-associated acyl-CoA thioesterase"
                     /protein_id="HLJ70230.1"
                     /translation="MTKDVRAKTAQGPAHRLALRVYYEDTDFSGRVYHAAYLRFLERG
                     RTEWLRARGFAQREMARDASIVFVVRRLTIDFIRPASMDDVLTIETRLTSLGAASFDF
                     AQAILREETILAEALVGVVALKGGRPARLPPELRARLFDHKADA"
     gene            1516..2463
                     /gene="fmt"
                     /locus_tag="VKU03_02865"
     CDS             1516..2463
                     /gene="fmt"
                     /locus_tag="VKU03_02865"
                     /EC_number="2.1.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004479 - methionyl-tRNA formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionyl-tRNA formyltransferase"
                     /protein_id="HLJ70231.1"
                     /translation="MLSPRAMRIVFMGAPAFAVPTLLEIIGQGHDVAAVYTRAPRPAG
                     RRGLELTKTPVHLAAERRAIPVFTPATLRNEAEQERFRGHAADVAVVVAYGLILPAPI
                     LAAPVHGCLNLHASLLPRWRGAAPIQRAIMAGDRETGVCVMRMEEGLDTGPVALVERV
                     AVAPEMTAGDLADRLARLGADLMTRALPALERGLLAFTPQPSEGVLYAKKIDKSEARI
                     DWTADAETVRNHIHGLSPSPGAYSEIDFGRGAERVKILRARAVVGRGAPGEVLDSALT
                     VACGSGAIQIVEAQRAGKTAMAGEELARGAQIAAGARFV"
BASE COUNT          381 a          957 c          917 g          420 t
ORIGIN      
        1 ctgcaggagc cgcgccagcc gcccatcccc gctgccttgc atttcgccgc cggcttcgac
       61 tggtccgcgc tcgggcctta cgccgtcttc gcccaaggcg ccgtccaggt cgcaaccgag
      121 atctggaatc agatcgcgcc caaggcctga acgctccgcg ccgcgtccga aacggcgcgg
      181 cgcgaacgtc ccttcttcgc ttcgcgctcc cgaagctctc gtccctccca aaaaaggtcc
      241 gttttctatg acgcgctcgt tttcgcgggc ggcggcgctc gccgccgcgc tcgtcctttc
      301 catcattctc gccgggtgca atcttgcgac gctggacgcc aagatcgccg ccgacgcctc
      361 ggcctcgctg ccgacgatct gctcgctcgg cgcctcggcg cacgccgcgt tctcgaccat
      421 cgccggaacc ggacaggtga gcaaggcggc cgccgccgac gaagccgcgg cctatgccgg
      481 cctgcaatcg ctctgcgcca atccgccgcg gaacgtgagc gcggcgatcg tcgaagccgg
      541 ctcgatctat gccgccatcg ccgactcgct cagcgcggcg gagagcgctt cgacgccgcg
      601 cgggcgatag gacgaacgaa atgagcgttg ctttcggcag cgatacgccc tggggcctgc
      661 tcgaatgggt cgccactggc ctgtcgacgg ccgtgctcgg ctccgccgct ttcgcctggc
      721 gcctcgccgc tcggctcgac acgaacagcg cggcgctgga atggcagaag gcggagctcg
      781 ccgcggtcaa gcaggcgcac gacgccgccg cttcgcgcct cgccgaccgg ctaacgcagc
      841 ttctcgacga tcaatgccgc ctgcgcgaga cggcggcggc gctgccgacc cgcgccgatc
      901 tgcgcgacat ggaggagcgt ctcggcgagc gcatccaggc gctggcggcg cggatcgacg
      961 gaattgtcga gaggggcgcg gcttaggcgt cggctttgtg atcgaacagg cgcgcgcgaa
     1021 gctccggcgg caaacgcgcc ggacggccgc ccttcaaggc gacgacgccg accagcgcct
     1081 cggccaatat cgtctcttcg cgcaaaatgg cctgcgcgaa atcgaaagac gcggcgccga
     1141 ggctcgtcag gcgggtttcg atcgtcagca cgtcgtccat cgaggcgggc ctgatgaaat
     1201 cgatggtcag ccggcggacg acgaacacga tggacgcgtc tctcgccatt tcccgttggg
     1261 cgaagccgcg cgcccgcagc cattcggtgc ggccgcgttc gaggaaacga agataggcgg
     1321 cgtggtacac gcggccggaa aaatcggtgt cttcataata gacgcgcagg gccagccgat
     1381 gggcggggcc ttgcgcggtt tttgcgcgta cgtctttcgt catggaaacc gcctatgcgg
     1441 gtttgcctca tccgttgtag ccgcggtcgg cgagcgcggc tactggcccg ccggttttga
     1501 ccctgcgcag gcgcgatgct atcgccgcgg gccatgcgca tcgtgttcat gggcgcgccg
     1561 gctttcgcgg ttccgacgct gctcgaaatc atcggccaag gccatgacgt cgcggcggtc
     1621 tatacgcggg cgccgcgccc ggccggccgc cgcggcctcg aactcaccaa gacgccggta
     1681 cacctcgccg ccgagcggcg cgccatcccg gttttcacgc ccgcgacttt gcgcaacgag
     1741 gccgagcagg agcgctttcg cggccatgcg gccgatgtcg cggtcgtcgt cgcctatggg
     1801 ctgatcctgc ccgcgccgat cctggcggcg ccggtccatg gctgtctcaa tctgcacgct
     1861 tcgctcctgc cgcgctggcg cggcgccgcg ccgatccagc gcgcgatcat ggccggcgat
     1921 cgcgagacgg gcgtctgcgt gatgcggatg gaggaggggc tcgacaccgg tccggtcgcg
     1981 ctcgtcgagc gcgtcgcggt cgcgcccgag atgacggccg gcgatctcgc cgaccggctg
     2041 gcgcggctcg gcgccgatct gatgacgcgc gccttgccgg cgctggagcg cgggctgttg
     2101 gcgttcacgc cgcagccgag cgagggcgtc ctctacgcca agaagatcga caaaagcgaa
     2161 gcgcggatcg attggacggc ggacgccgaa acggtccgca atcatatcca cggcctgtcg
     2221 ccttcgccgg gcgcctattc cgagatcgat ttcggccgcg gcgcagagcg ggtgaaaatc
     2281 ctccgcgcgc gcgcggttgt gggccgcggc gcgccgggcg aggttctcga cagcgcgctg
     2341 accgtcgcct gcggaagcgg cgcgatccaa atcgtcgagg cgcagcgggc gggcaagacc
     2401 gcgatggccg gcgaggaatt ggcgcgcggc gcgcagatcg ccgccggcgc gcgcttcgtt
     2461 tgagcgtcgt ctcgtgacgc gcctccattc gccactcgcc acatcatcgc ctcggggcgc
     2521 gctcgtccgg cggcgcgagg ctcatcgccc cgcgaagaag tccagcagcg ccgccgcggt
     2581 ctcgtcggga ttttcctccg gcaggaaatg tccgccggtc acggctcgcc ccctcgcttg
     2641 cgccgcccag cgccgccaga tcgtcagcgg atcgt
//
LOCUS       DATGJW010000097         3694 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_639431, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000097 DATGJW010000000
VERSION     DATGJW010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3694)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3694)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3694
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_639431"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1552
                     /locus_tag="VKU03_02870"
     CDS             110..1552
                     /locus_tag="VKU03_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HLJ70232.1"
                     /translation="MNTPRGELEVERLKDPVLLGRAGYALAAHDGYFREVLNALPAAI
                     YITDADGLIIYYNTAAGELWGHSPPLGTSQWCGSWKLFCPDGTPLPHDQCPMAVALKE
                     RRPIRGVEAVAERPDGVRAPFMPYPTPFFDESGALLGAINMLVDMTGLRRAEDDARLL
                     ASVVETSDDAIVTKNLDGIITSWNRGAQRIFGYTAEETIGKSITILIPDHLQDEEPQI
                     LARIRAGQRIDHYETIRRRKDGTLLNVSLTISPLKNSHGVIVGASKIARDITEQRRAQ
                     EQQELLVREMNHRIKNLFSLASGVVNLSARSAGTPQELAAAVRDRLAALARAHDLTLL
                     KTPDGAIGDHPATLHALIRTIVSPYEEQTGEAGERVRIRGADAALSAPSVTSLALLLH
                     EFATNAAKYGALSMPGGAVDVECAEEGDHLILTWTESGGPRVEKAAEGEGFGSLLART
                     AVKGQLDGDIARDWRPEGLVIRLSVSRNRL"
     gene            1705..2688
                     /locus_tag="VKU03_02875"
     CDS             1705..2688
                     /locus_tag="VKU03_02875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70233.1"
                     /translation="MPAAPASRKHYRLGALYSIATSALLATQEPFSSLAAKRLAPALF
                     VCLTQAALLLSVPLLISPSGSRRDFIALLATPTNYWKLAVLFLIGLTGLLLYNAGLSN
                     AHPIIIAAILNLSPLWAAAVALLISKKPIPVSPGIFFGCLAVAFIGAMTIAWSQASGS
                     NAPTLAALAASLGHTSWFYAIPIPILFALSGTLVGHWFGKFDPAAAIAANFAVSATIL
                     IPATVALLALHPAPAGREHAAAAIVFLMVGTLASAAAGRVLYQVALAATKNDNGFVTM
                     FFLTVPGLTTLISIPLSWWIPELRFVASPLFFSGLALVAAPLAFFSVKAWR"
     gene            complement(2796..3563)
                     /locus_tag="VKU03_02880"
     CDS             complement(2796..3563)
                     /locus_tag="VKU03_02880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Bax inhibitor-1/YccA family protein"
                     /protein_id="HLJ70234.1"
                     /translation="MSDYDRFANARYGAGVARSGVGAVDQGLRAYMIGVYNYMMLGLG
                     ITGLFALGAFKLGVVEKAGQLFLTPFGQAIFHSPLRWVIVLSPLAVVFYISARINAMS
                     AASARNAFFLFSALIGLSMSSLLLVYTGASIGRVFFVTAAAFAGLSLYGYTTRRALSG
                     MGSFLIMGLWGVIIAGLVNLFLHSSGLMWALSLLSVVIFAGLTAWDTQAIKESYYSGD
                     GYEMVQKKSIFGALRLYLDFINLFQALLMLTGSNRNN"
BASE COUNT          645 a         1238 c         1169 g          642 t
ORIGIN      
        1 cacgaataag tgattggccg cgtctcccga ctttgatcga ttctaaactc taaccacggc
       61 ggcggccggc gatgcgtttg cgctgggacg ttccctcagg aggcgcttga tgaacactcc
      121 gcgcggagag ttggaggtcg agcgcctgaa agatccggtc ctgctcggcc gggccggtta
      181 cgcgctcgcc gctcatgacg gttattttcg cgaagtgctg aacgctctgc cggcggcgat
      241 ctacatcacc gacgccgacg gattgatcat ctattacaat acggccgcgg gcgagctttg
      301 gggccattct ccgccgctcg gcacgagcca atggtgcggc tcgtggaagc tgttttgccc
      361 ggacggaacg ccgctgccgc acgatcaatg cccgatggcc gtcgccctca aggagcggcg
      421 gccgattcgc ggcgttgagg ccgtcgccga gcggccggac ggcgtcaggg cgccgttcat
      481 gccctatccg acgccgttct tcgacgaatc cggcgcgctg ctcggcgcga tcaacatgct
      541 ggtcgacatg accggcctga ggcgcgccga agacgacgcg cggctcctcg catcggttgt
      601 cgaaacctcc gacgacgcga tcgtcaccaa gaatctcgac gggatcatca cgagctggaa
      661 tcgcggcgcg cagcggattt tcggctacac ggccgaggag acgatcggca agtcgatcac
      721 gattctcatt cccgaccatc ttcaggacga ggagccgcaa atcctcgccc gcatccgcgc
      781 cggccagcgg atcgaccatt acgagacgat ccgccggcgc aaggacggga cgttgctcaa
      841 tgtttcgctc accatctcgc cgctgaagaa ttcgcacggc gtgatcgtcg gcgcttcgaa
      901 aatcgcgcgc gacatcaccg agcagcgacg ggcgcaggag cagcaggaac tgctggtccg
      961 ggagatgaat caccggatca agaacctgtt cagcctggcg agcggcgtgg tgaatttgag
     1021 cgcgcgaagc gccggcacgc cgcaggaatt ggccgccgcc gttcgcgacc gccttgccgc
     1081 cttggcgcgc gcgcatgact tgaccttgct caagacgccg gacggggcga tcggcgatca
     1141 tcccgcgacg ctccacgcgc tgatccggac gatcgtctcg ccttacgagg agcagaccgg
     1201 cgaagccggc gagcgggtcc gcattcgcgg cgccgacgcc gccctgtccg cgccgtcggt
     1261 gacgagcctg gcgctgcttc ttcatgaatt tgcgaccaac gccgccaaat acggcgcctt
     1321 gtccatgccg ggcggcgcgg tcgacgtgga atgcgccgag gagggcgacc acctgatcct
     1381 gacctggacc gagagcggcg ggccgcgcgt cgaaaaagcc gccgagggcg aaggcttcgg
     1441 cagcttgctg gcgcgaaccg cggtgaaggg acagctcgac ggcgacatcg cgcgcgattg
     1501 gcgcccggaa gggctcgtca tcaggctttc ggtctcgcgc aaccgcctgt agccgcggtc
     1561 ggcgaccgcg gctacagccg ccgcggcttc ccggaacatt tccgccttcc aacctgttgg
     1621 tacgtcgacc tgccacccgg cgccggcgaa gctcggcgcc gacgcatccg ccatcccaaa
     1681 cgccgcggct gggcgaggaa gttcatgccg gccgctcccg cctctcgaaa gcattaccgg
     1741 ctcggcgctc tctattcgat cgccacttcc gcgcttttgg cgacgcaaga accgttttca
     1801 tccctcgcgg cgaaaagact ggccccggcg ctcttcgtct gcctgacgca ggcggccttg
     1861 ctcctttccg tgccgctgct gatctcgccg tccggcagcc ggcgcgattt catcgcgctg
     1921 ctcgccacgc caacgaacta ttggaagctc gccgtccttt tcctgatcgg actgaccggg
     1981 ctgcttcttt acaatgccgg cctcagcaat gcgcatccga tcatcatcgc cgcgatcctc
     2041 aacctgtcgc cgctctgggc ggcggcggtg gcgctgctga tttcgaaaaa gccgattccg
     2101 gtctcgccgg gaatcttctt cggctgcctg gccgtcgctt tcatcggcgc gatgaccatc
     2161 gcctggagcc aggcgagcgg gtcgaacgcg ccgacgctcg ccgctctggc ggcgagcctc
     2221 ggccatacga gctggttcta cgccattccg atcccgatcc tgttcgcgct gagcggcacc
     2281 ctggtcggcc attggttcgg caagttcgat ccagcggcgg cgatcgccgc caatttcgcc
     2341 gtctcggcga cgattctcat cccggcgacg gtcgcgctcc tcgccttgca tccggcgccc
     2401 gccggacggg aacatgccgc cgcggccatc gtctttctga tggtcggcac gctcgcctca
     2461 gccgcggcgg gccgggtgct ctatcaggtc gccttggccg cgacgaagaa cgacaatggc
     2521 ttcgtcacca tgttctttct gaccgtgccg ggcctgacca cgctgatctc gatcccgctc
     2581 tcctggtgga ttccggaatt gcgcttcgtc gccagcccgc tctttttttc aggcctggcg
     2641 ctggtcgcgg cgccgctggc gtttttctcg gtgaaggcgt ggcggtgagc ggggacgtag
     2701 cgtcccgtca ctgcgcagtg acggtcgtct cacgcaaaaa cccctggcgc ctttcggagc
     2761 caggggtctc gtcaattcgt gtctgtcgac ggcgcttagt tgttccggtt cgagccggtc
     2821 agcatgagca gcgcctggaa caggttgatg aagtcgagat agaggcgcag cgcgccgaaa
     2881 atcgacttct tctgcaccat ctcatagccg tcgccggaat agtagctctc cttgatcgcc
     2941 tgggtgtccc aagcggtcag gccggcgaaa atgacgacgc tgagcaacga cagcgcccac
     3001 atcaagccgg agctatgcag gaacaggttg accaagccgg cgatgatcac tccccacagg
     3061 cccataatca ggaacgagcc catgccggaa agagccctgc gggtcgtgta gccgtaaaga
     3121 ctgagacccg cgaaagcggc cgccgtcacg aaaaagaccc gtccgatcga agccccggta
     3181 tagaccagga gcaacgagga catggagagg ccgatcaaag ccgagaagag gaagaaggcg
     3241 ttccgcgccg acgccgcgga catcgcgttg atccgcgccg aaatgtagaa gacgacggcg
     3301 agcggcgaca gcacgatcac ccagcgcagc ggcgaatgga aaatcgcctg gccgaacggg
     3361 gtcaggaaga gctgcccggc cttttcgacg accccgagct tgaacgcgcc gagcgcgaac
     3421 aagccggtga tgccgagccc gagcatcatg taattgtaga cgccgatcat ataggccctc
     3481 aggccctgat cgactgcgcc cacgccggac cgggcgacgc cggcgccgta acgagcgttc
     3541 gcaaagcggt catagtccga cattgaaaga cctcgtgaga tgttcgcgcg accgcctcgc
     3601 ggatatcgcg gcgcggggtc gcgcctcatc atatggcccc ggccccggcc ggcgcaaggg
     3661 accggcgctc ggcttgcgcg aactctctgt ttta
//
LOCUS       DATGJW010000098         5869 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_536346, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000098 DATGJW010000000
VERSION     DATGJW010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5869)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5869)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5869
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_536346"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            209..1495
                     /locus_tag="VKU03_02885"
     CDS             209..1495
                     /locus_tag="VKU03_02885"
                     /inference="COORDINATES: protein motif:HMM:TIGR03808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03808 family TAT-translocated repetitive
                     protein"
                     /protein_id="HLJ70235.1"
                     /translation="MIDRRSLFAAALALPALARPRPALGEPLVDAAPRLQRLIDEAQR
                     SGRVVEIPAGTSRVSQLKLSGDVRLVGAGRSSRLVGLGAGPMLSIDRAARVAIENVAF
                     GGDGRAPSEAGLIEARDVADLRVSDCAIEDGRGYGLKLERCGGRIERSSFRNLAQSAL
                     HSLDATGLAIADNLIADCGANGLQVWRSAPGYDGAIIRGNRIERIRADPGGDGPYGNA
                     ISAFRAGGVSCTGNVVRQAAFSAIRFNSSPDAVIADNNCFDIGETALYVEFAFQGAVV
                     SANLVDGASAGISVTNIDQGGRLASVTGNVLRNLVKPAPQGVANYGVGVHAEADTVVS
                     GNAVDNARFAGLTLGYGIGLKDVIATGNAINDCGYGIAVSVAPGAGGATIRDNRISGS
                     RRGAIVGMAWEKVVSADLIAEAAKYPQLTISGNGLR"
     gene            complement(1500..1685)
                     /locus_tag="VKU03_02890"
     CDS             complement(1500..1685)
                     /locus_tag="VKU03_02890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6496 domain-containing protein"
                     /protein_id="HLJ70236.1"
                     /translation="MAKTSRSASSDVRRAMHRRKKGTLKSGRSGKTVKSRKQAIAIGL
                     SEARRKGKKVAPKKKAS"
     gene            complement(1757..2227)
                     /locus_tag="VKU03_02895"
     CDS             complement(1757..2227)
                     /locus_tag="VKU03_02895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="group III truncated hemoglobin"
                     /protein_id="HLJ70237.1"
                     /translation="MSLSIKSAAARRAEFVAARKAETGIDEAMIDRLVRAFYARVRED
                     ELLGPVFAARIEDWEPHLRQMGAFWSSVTLMTGAYHGEPMRKHLPLPIDARHFDRWLA
                     LFAETARELCPPKAADCFIERAQKIAESLELGLAGANGVLLAKGERFRRDCAPD"
     gene            complement(2224..2772)
                     /locus_tag="VKU03_02900"
     CDS             complement(2224..2772)
                     /locus_tag="VKU03_02900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rrf2 family transcriptional regulator"
                     /protein_id="HLJ70238.1"
                     /translation="MRGNDTGWGWRFALKEVYEAHLFTRPGPMRLTRHTDYALRLLML
                     LAAEPDATHTIEETARRYGVSRNHLMKVTQTLIKAGFVAGARGRGGGLALPRPAADIN
                     VGAVVRATEENLDLVECFDSSGNTCRLTPACGLRAPLREALSAFLGVLDQYSLADLVG
                     NPASARRMRRLLGAAPRMEARP"
     gene            complement(2856..3599)
                     /locus_tag="VKU03_02905"
     CDS             complement(2856..3599)
                     /locus_tag="VKU03_02905"
                     /inference="COORDINATES: protein motif:HMM:NF015866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0055070 - copper ion homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis protein CutC"
                     /protein_id="HLJ70239.1"
                     /translation="MPLFEICIDSVAGARAALQAGAARVELCAALLEGGITPSPGMIR
                     QAVAAAAGRLKVHVMIRPRGGDFLYDEDEFAVMRRDVLAAKEAGADGVVIGLLDADGG
                     VDEKRSAELIAAARPLSVTFHRAFDVARDAEQSLDALIRLGADRLLTSGQEPSVLEGA
                     PLIRRLIGRAGGRLVVMPGGDITPRNAARIAAETGAEEFHFAAFAPAASAMRWRNEAV
                     FMGGALRPPEYDRAATTASAVRAVMAAFG"
     gene            4103..4813
                     /locus_tag="VKU03_02910"
     CDS             4103..4813
                     /locus_tag="VKU03_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HLJ70240.1"
                     /translation="MARLARVVVPGLPHHVTQRGNGRARVFFSDADYALYRDLLAAAC
                     REARVEVWAWVLMPNHVHLILVPADADGLRRALARVHRRYAGVVHARRRRSGHFWQGR
                     FGSVAMDEEHLAAALRYVSLNPVRARLVKRAQDWRWSSAKAHLGLAEDGVTLVKPALS
                     RFPRFADLLAEPANAEALDRLRQAESVGRPLGSPAFLARIEKRLSRPLAAKKRGPKPA
                     KAEDKAPRRTKGKSARSR"
BASE COUNT          936 a         1994 c         1976 g          963 t
ORIGIN      
        1 ctgccgattg ccgactgccg aacggcttcc aaattcgaac ggcgcgtcta agctcgaaat
       61 atgagtcccg acggcagtcg gcagatcggc agtcggcagt cgggatttcg cctagaccga
      121 ctgccgattg ccgactgccg actgccgaat ggcttccaaa ttcgcacggc gcgtctaagc
      181 tcgaaaatat gagtccggac gcgctgtcat gatcgatcga cgaagcctct tcgccgccgc
      241 gctggcgctg ccggcccttg cgcggccgcg gccggcgctg ggcgagccgc tcgtcgacgc
      301 cgcgccgcgt ctgcagcgcc tgatcgatga ggcgcagcgc agcggccgcg tcgtcgagat
      361 tcctgccggg acgagccggg tgtcgcagct caagctatcc ggcgatgtgc ggctcgtcgg
      421 cgccggacga tcgagccgcc tcgtcggcct cggcgccggc ccgatgctgt cgatcgatcg
      481 cgccgcgcgc gtcgcgatcg agaacgtcgc gttcggcggg gacggccgcg cgccgagcga
      541 ggccgggctg atcgaagcgc gcgacgtcgc cgatctgcgc gtttcggatt gcgcgatcga
      601 ggacggccgt ggctatggcc tgaagctcga acgctgcggc ggacgcatcg agcgctcgtc
      661 cttccgcaac ctcgcgcaat cggccctgca tagtctcgac gcgaccggcc tcgctatcgc
      721 cgacaacctg attgccgatt gcggcgccaa cggtttgcaa gtctggcgct cggcgccggg
      781 ctatgacggc gcgatcatcc gcggcaatcg gatcgagcgc attcgcgccg atcccggcgg
      841 cgacgggccc tatggcaatg cgatcagcgc gtttcgcgcc ggtggggttt cttgtaccgg
      901 caatgtcgtc cgccaggcgg cgttttcggc gatccggttc aattcgagcc ctgacgcggt
      961 catcgccgac aacaattgct tcgatatcgg cgagaccgcg ctctatgtcg aattcgcctt
     1021 ccaaggcgcc gtcgtctcgg ccaatctcgt cgacggcgcc tccgccggca tttcggtgac
     1081 caatatcgac cagggcggcc gtctggcgag cgtgaccggg aacgtcctgc gcaatctcgt
     1141 caagccggcg ccgcaagggg tggcgaatta cggcgtcggc gttcacgccg aggcggacac
     1201 cgtcgtcagc ggcaacgccg tcgacaatgc gcgtttcgcc ggattgacgc tcggctatgg
     1261 gatcggcctc aaagacgtga tcgcgacggg caacgcgatc aacgattgcg gctacgggat
     1321 cgcggtctcg gtcgcgccag gcgccggcgg cgcgacaatc cgcgacaacc gcatcagcgg
     1381 atcgcgccgc ggcgcgatcg tcggtatggc ttgggagaaa gtcgtctccg ccgatctgat
     1441 cgccgaggcg gcgaaatatc cgcaattgac gatttcgggg aacggcctgc gctgaagcgt
     1501 cacgacgcct tcttcttcgg cgcgaccttc tttcccttcc gcctcgcctc cgacaatccg
     1561 atggcgatcg cctgcttgcg gctcttcacg gtcttgccgc tgcggccgct cttgagcgtt
     1621 cctttcttgc gccgatgcat cgcgcggcgc acgtcgctgg acgctgatct cgatgtcttg
     1681 gccacggctc actcctctgt acccggcccc ggcgtccaac gcgcgttctt tgtggttgtt
     1741 cctgagtgag ctgcgctcaa tcgggcgcgc agtcgcgacg gaatcgctcg cccttggcga
     1801 gcagcacgcc attcgcgccg gcaaggccaa gctccaggct ctcggcgatc ttttgcgccc
     1861 gctcgatgaa gcaatcggcg gcttttggcg ggcaaagctc gcgggcggtt tccgcgaaca
     1921 gggcgagcca gcggtcgaaa tggcgcgcgt cgatcggcag cggcagatgc ttgcgcatgg
     1981 gctcgccgtg ataggcgccg gtcatcaacg tgaccgagga ccagaacgcg cccatctggc
     2041 gaagatgcgg ctcccaatcc tcgatccgag ccgcaaagac cgggccgagc aattcgtcct
     2101 cgcgcacccg cgcatagaag gcgcgcacca ggcggtcgat catcgcctcg tcgattccgg
     2161 tctcggcctt tctggccgcg acgaactcgg cgcggcgcgc cgccgccgat ttgatcgaaa
     2221 ggctcatggc cgcgcctcca tgcgcggcgc ggcgccgagg agacgccgca tccgccgcgc
     2281 cgaagccgga ttgccgacga gatcggcgag cgaatattga tcgagcacgc cgagaaaggc
     2341 gctgagagcc tcccgcagcg gcgcgcgcag gccgcaggcc ggcgtcagcc ggcacgtatt
     2401 gccgctggaa tcgaaacact cgacgagatc gagattttcc tcggtcgccc gaaccactgc
     2461 gccgacattg atgtcggcgg ccggccgcgg cagggcgagg ccgccgccgc ggccccgcgc
     2521 gccggcgacg aagccggctt tgatcagggt ctgcgtgact ttcatcaaat gattgcgcga
     2581 gacgccgtag cgccgcgccg tctcctcgat cgtatgcgtc gcatcgggct cggcggcgag
     2641 cagcatgagc agacgcaggg cgtagtcggt gtggcgggtg aggcgcatcg gtccgggacg
     2701 ggtgaaaaga tgcgcttcat atacctcttt aagggcgaac cgccaacccc atcccgtgtc
     2761 attcccgcgc attgtcactc ccgcgcgaac gggaattcag cacagcctct cgcgcaggcg
     2821 cagctcctgg atttccggcg caacgcggaa tgacgctaac cgaacgccgc catcacggcg
     2881 cggacggcgc tcgccgtcgt cgcggcgcgg tcatattcgg gcggccgcaa ggcgccgccc
     2941 atgaacaccg cctcattgcg ccagcgcatc gcgctcgccg ccggcgcgaa agcggcgaag
     3001 tggaattcct cggcgccggt ctcggcggcg atccgggcgg cgttgcgcgg cgtgatgtcg
     3061 ccgccgggca tgacgacgag ccggccgccg gcgcggccga tcaggcggcg gatgagcggc
     3121 gcgccttcga gcacgctggg ttcttggccg gaggtcagca accggtcggc gccgagccgg
     3181 atcagagcgt cgaggctttg ctccgcatcg cgcgccacgt cgaaggcgcg atgaaaggtc
     3241 acggagagcg gccgcgccgc ggcgatcaat tcggcgctgc gcttttcatc gacgccgccg
     3301 tcggcgtcga gcaggccgat gacgaccccg tcggcgccgg cctccttcgc cgcaaggacg
     3361 tcccgccgca tgaccgcgaa ctcgtcctcg tcatagagaa agtcgccgcc gcgcggccgg
     3421 atcatgacat gaaccttgag ccgcccggcc gccgcggcga cggcttggcg gatcatgccg
     3481 ggcgagggcg tgatcccgcc ttcgagcaag gcggcgcaga gctccacccg cgccgcgccg
     3541 gcttgaagcg ccgctctcgc cccggcgacg ctgtcgatgc agatttcgaa gagaggcatg
     3601 tccccggcgc cgtccgtctt gcgccgccaa gcgcggctga aacaagatga cggcggcgcc
     3661 cgcggaacgc aagcggagcc gccgccggcg gcttcgcaat gagcggcggg accgtaccgt
     3721 ctcacgccgc ggcgagcggt cggcaggacg gggcgaccga tgaaacagaa acgagcgcgg
     3781 cgcccggccc ccctctcccc gcaaagcggg gagaggggct tcgacgccgc gcttctttat
     3841 tgagcgcgat gcgacgcttt actcgccgga gagcgcaagc aatcgctctc tgagccattg
     3901 atgcgccggg cgccggtggg tgcgttcgcg ccacacgagg ccgacaaagc tagtgaccgt
     3961 gacaggtgac cgtgacagtg cattcaattg gtcgaggcgc gccggatgca gcaaggctca
     4021 tgatcgcgac gcggcgctgc gagacattac ggtgacggtg cgctcaattg acattcgccc
     4081 ggcgatgccg gaggctgcgg tcatggcccg attggcgcgc gtcgtcgtac ccggtctgcc
     4141 gcatcacgtc acgcagcgcg gcaatggccg cgcgcgggtg tttttctccg acgcagacta
     4201 tgcgctctat cgcgatcttc tcgccgcggc gtgccgggag gcgagggtcg aggtctgggc
     4261 ctgggtgctg atgcccaatc acgtccatct cattcttgtc cccgccgacg ccgacgggct
     4321 gaggcgggcg ctggcgcgcg tccatcgccg ctatgccggc gtcgtgcatg ctcgccgcag
     4381 gcgcagcggg catttctggc agggccgctt cggttcggtc gcgatggacg aggagcatct
     4441 ggccgccgcg ttgcgctatg tctcgctcaa tcccgtgcgg gcgcgcttgg tcaagcgggc
     4501 gcaggactgg cgatggtcga gcgccaaggc gcatctcggc ctcgccgagg acggcgtgac
     4561 tctcgtcaag ccggcgctca gccggtttcc gcgcttcgcc gatctgctcg ccgaaccggc
     4621 gaacgccgag gcgctcgatc ggttgcgcca ggccgaaagc gtcggccggc cgctcggctc
     4681 gccggccttt ctcgcccgca tcgaaaagcg cctttctcgc ccgctcgccg ccaagaagcg
     4741 cggtccaaag ccggcgaagg ccgaagacaa ggcgccgaga cggacgaagg ggaaaagtgc
     4801 gcggtcacga tagtattaaa gagaagggcc gctcacgcgg ccctccgtca tcggacgaga
     4861 tcaatcgcaa ttggttttcg tccgggtctc actgtttccc gccgcatctt cccgtttgac
     4921 agacttcgtc tgacacccct cgtcgacgct tcgatgctcg ataatcacgc gccgatcggg
     4981 gggaggcgga ggaggattgt ctgtgccgat aacgcacgcg cctgccggac cgatatggaa
     5041 accgaccgcg caatcggcgt gatggaggtc ggtcggattt gcgtcctggc cgtaatgggc
     5101 cggagacatc gctgcttgag tcggcccgac cgagatgaac gcggctagag cggaggcagc
     5161 cgcgcaacgc aggatgatgt ttcggataag catggtcccg cctttcgtca gctcgctctt
     5221 tctctgaaac gagctttccg cccgctgcaa cctcggtcgg gcaaaattgt tccgcgcccc
     5281 ctgaaacgca cgggaactcc aaaggggctt tttttccgcg acttaggcct ccgtcccagg
     5341 aacaaaagcg cgccgctgcc cgcgccgaca ttggcgaagg gcgacccggc tttgttcccc
     5401 tcgaccatcg ccggcccgac gagaggcgtt ataccggcgc ctcgagccgt agaggacaga
     5461 ccgccgtatg gcctgcgtcc cagcgcattg catccgtcgc atcattacgg tgatggggtg
     5521 gacggcgccc cgagcggcct caaggagcgg ctttacatct gtacttcaag ccgcaagcgg
     5581 accgaccccg acatcgcccg cgtcctgaag gcccttgcga agcggctcgc gcaaatcggc
     5641 gccggcggcg agcggtcggc gggacgaggc gaccgatgaa acagaaacga gcgcggcgcc
     5701 cggcccccct ctccccgcaa agcggggaga ggtacggaga ggggcttgtc gccttgccgc
     5761 gcctgaggat aaccgaccag cccctcaccg tgcctctccc cgcttcgctt cgctccgcgg
     5821 ggagaggggc ttcgacgccg cgcttcttta ttgagcgcga cgcgacgct
//
LOCUS       DATGJW010000099         8088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1055612, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000099 DATGJW010000000
VERSION     DATGJW010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8088
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1055612"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..482
                     /locus_tag="VKU03_02915"
     CDS             <1..482
                     /locus_tag="VKU03_02915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ70241.1"
                     /translation="RVIASEPRKGEKIPVKTATHIAGTMEFVSGAAVSIAMSFDVPKH
                     MHKPLEIYGTKGSLIVPDPNYFDGQIEIATDGDWTPVETEHSYADGNYRIIGAADMAH
                     AIRKNRPHRASGDLAFHVLEVMEAFEDSSRSGAFVEIKSRPERPASLPVGLKTGELD"
     gene            515..1159
                     /locus_tag="VKU03_02920"
     CDS             515..1159
                     /locus_tag="VKU03_02920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003510737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThuA domain-containing protein"
                     /protein_id="HLJ70242.1"
                     /translation="MREALIVWGGWSGHEPEIGAHIVADMLKKEGFATYIENTTEAFA
                     DPAIADMSLIVPIYTMSKIEKEELDNLTRVVRGGVGLAGYHGGMADAFREAVEYHFMV
                     GGQWVAHPGNIIDYRVDIVKPDDPIMQGLPASFPYRSEQYYMHVDPSNEVLATTTFSG
                     EHADWVEGVVMPVVWKRRHGKGRVFYCSLGHVTKEFDVPEMRTIIQRGLLWAAR"
     gene            1334..1909
                     /locus_tag="VKU03_02925"
     CDS             1334..1909
                     /locus_tag="VKU03_02925"
                     /inference="COORDINATES: protein motif:HMM:NF012415.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CAP domain-containing protein"
                     /protein_id="HLJ70243.1"
                     /translation="MRPACAGALIAALCLSACGGGEPLLSGPIAPDVARRVAAVRLDP
                     AAAAKMLTAYRAGHGLGAVRLDPALTAMAQRQADAMVAANELSHDVAGGFTARVLAAG
                     LDTPRAAENLGGGYYSLEEAFTGWRHSPEHNANLLMPQATRFGIAIAKDPRTHLRVYW
                     AMELAAEPEKPARGEAAGLISTLGGAVVANP"
     gene            complement(2013..2966)
                     /locus_tag="VKU03_02930"
     CDS             complement(2013..2966)
                     /locus_tag="VKU03_02930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70244.1"
                     /translation="MSSNRALVALAALILFGALRYDHFLGLYNVFSVLRYNSMFALTG
                     LGMCFVIITGGIDLSVGAVAALASVISAILSPYGLIAGLAGGLGSGLIIGAINGAMVT
                     RLKILPFIATLAGTLMAGGSALLAAHNQTVGVSYDTAFTAIGQGDFLGFPIPAWIAIL
                     AYVVGWILLNYSPFGRYALAVGGEPDAARLMGLPVERVEFFTYVQSGLLASLAGVILA
                     AQFGAGQPTEGVGWELFSIAAVVVGGTLLSGGRGSVGATLAGVLLLGLVFNILNFENG
                     MGWISLSAYWQSVIRGVFLFVVVIAGAGGKARRATEAGATG"
     gene            complement(3024..3926)
                     /locus_tag="VKU03_02935"
                     /pseudo
     CDS             complement(3024..3926)
                     /locus_tag="VKU03_02935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121100.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            complement(4006..5571)
                     /locus_tag="VKU03_02940"
     CDS             complement(4006..5571)
                     /locus_tag="VKU03_02940"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ70245.1"
                     /translation="MSAIDAASPAPILSMEGIEKAFGGSKALAGARIDVAPGEGMALV
                     GQNGAGKSTLIKVLTGVHRKDAGTIHFAGRPVEFGSPQQAQRGGIATIYQEINLIPYR
                     SAAENICLGKEPRRYGMIDWRRMNAEAADILRRFSVEIDVRRPLMTFSTAIQQMVAIA
                     RAVSYSARLVIMDEPTSSLDDRECEVLFDVIRRLKQDGVSVIFVSHRLDELYQVCERV
                     TVMRDGRTVLVDRLANLDKLQLVAAMLGRDLAAVRAKGATAFSRGRAKVGAEILRAEG
                     LKIGRRVRNARLAVRAGEIVGLAGLLGSGRTEVARAIFGADPPDAGRIDIAGEKASLG
                     EPIEAIRRGLGYCSEDRKRDGVIPNMSVGENLTLALLPRLTRAGVVDEAKQREVVAKF
                     MARLGVKAASADQKIRELSGGNQQKVLLARWLCTNPKLLILDEPTRGIDVGAKGEIQS
                     LIDELAAEGLGVLMISSELEEIVEGADRVFVLRDGETAAELSGDSISEPNIMAVMAHG
                     GSEDGVIAEKKLD"
     gene            complement(5649..6623)
                     /locus_tag="VKU03_02945"
     CDS             complement(5649..6623)
                     /locus_tag="VKU03_02945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008121103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ70246.1"
                     /translation="MIITRRRLLITSAAGAAGLAMGSAWAAPALKQKATYKVGFAQTE
                     SNNPWRLAETASMQDEAKKLGYQLVYTDAAGSAAKQASDVNSMIAQGVDAIFLPPRED
                     KPLVPVIKAAAKAGIPVFLIDRDVDHSMAKPGVDYVTFIGSNFVEEGQRVAAWLAKAV
                     DGKAKIIELEGTTGSSPANDRKKGFDEAIKKWPGMVILASQSGDFARDKGRQVAETLL
                     QAHPDVTAIYAHNDEMALGAIAALEAAGKKPGKDVLVVSIDGEKDAVQAIIDGKLGCT
                     CECNPRFGPKAFQTMKDYAAGKTIPPYIKNEDRFFDSSNAKEVLASAF"
     gene            6839..7933
                     /locus_tag="VKU03_02950"
     CDS             6839..7933
                     /locus_tag="VKU03_02950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016557792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLJ70247.1"
                     /translation="MSERPGAIADIGQPGKAPLGPDAAARRRPTMIEVAAEAGVSQTT
                     VSLVLNDAQGARLSTETRERVFEAARRLGYRLARRNAPALAGAATIGFVVNELSTDPW
                     TAIAMDGARDKAWEHGLTISAAVTRGDAEMEQAVLAQMTAQPMLGLIYGTINTRRITP
                     PRALYRVPTVLLNCYVADRSLPSVVPSEVAGGHVATRRLIRAGHRRIGYINGEALMDA
                     CRDRLKGYRQALAAADLPYDPDLVRPGNWEPSAGYEQTFALMRLAEPPTAIFCANDLM
                     AVGCIEALKEMGRRVPEDVAVVGYDDREIAQFTHPPLTTVLLPHYEMGAEAAAYLIDY
                     ARRPPARPPQIKVECPLIERKSVAPGGGEA"
BASE COUNT         1456 a         2651 c         2658 g         1323 t
ORIGIN      
        1 agcgcgtcat cgccagcgag ccgcgcaagg gtgaaaaaat ccccgtcaag accgcgacgc
       61 acatcgccgg gacgatggag ttcgtcagcg gcgccgccgt ctcgatcgcc atgagtttcg
      121 acgtgcccaa acatatgcac aagccgctcg aaatttacgg caccaagggc tcgctcatcg
      181 tgccggatcc gaactacttc gatggccaga tcgaaatcgc gacggatggg gattggacgc
      241 cggtcgaaac cgaacattcc tatgccgacg gcaattatcg catcatcggc gcagcggaca
      301 tggcgcatgc gatccgcaag aaccgcccgc atcgcgccag cggcgacctc gcttttcacg
      361 tgctcgaagt gatggaggcg ttcgaagatt cgtcgagatc gggcgctttc gtcgagatca
      421 agagccggcc cgagcgccct gcctcgctgc ccgtcggcct caagaccggc gagctcgatt
      481 agccgcggcg agacaaattc gaaaggagtt aaaaatgcgg gaagcgctta tcgtctgggg
      541 aggttggagc ggccacgagc ccgaaatcgg cgcgcatatc gtcgccgaca tgttgaagaa
      601 ggaaggattt gcgacctata tcgagaatac gaccgaagcc ttcgccgatc cggccatagc
      661 cgatatgagc ctgatcgtgc cgatctatac gatgtcgaaa atcgagaagg aggagctcga
      721 caacctgacg cgggtggtca gaggcggcgt cgggctcgcc ggctatcacg gcggcatggc
      781 cgacgcgttc cgcgaggcgg tcgagtacca tttcatggtc ggcggccaat gggtcgccca
      841 tcccggcaac atcatcgact atcgcgtcga catcgtgaag cccgacgatc cgatcatgca
      901 agggctgccg gcgagctttc cgtaccgttc cgagcaatat tacatgcatg tcgacccgtc
      961 caatgaagtg cttgcgacga ccacgttttc cggcgagcac gccgactggg tcgagggcgt
     1021 ggtcatgccg gtcgtctgga agcgccgtca cggcaagggc cgcgtgttct attgctcgct
     1081 cggccatgtg acgaaggaat tcgacgtgcc ggaaatgcgc acgatcatcc agcgcggcct
     1141 gctctgggcg gcgcgataag cacgcgctta tcgtccttgc gagcgcaacg aagcaatcca
     1201 atcatcgccc gccgccctgc gaatagattg cttcttcccc tcgctccgct cgcagtcccg
     1261 cgatgacggc gcgcgctaag tttcctgaaa tcaatttcgt gctaaacctc gccacgtcat
     1321 tgtgaggggc ttcatgcgtc ctgcctgtgc cggcgcgctc attgccgcgc tttgtctttc
     1381 cgcctgcggc ggcggggagc cgctgctcag cggcccgatc gcgccggacg ttgcgcgccg
     1441 ggtcgccgcc gttcgtctcg accccgccgc agcggccaaa atgctcaccg cgtaccgggc
     1501 gggccatggc ctcggcgccg tgcggctcga cccggcgctg acggcgatgg cgcagcgcca
     1561 ggccgacgcg atggtcgcgg cgaacgagct ttcgcacgac gtcgcgggcg ggttcacggc
     1621 gcgagtcctc gccgccggcc tcgatacgcc gcgcgccgcg gagaatctcg gcggcggcta
     1681 ttattcgctg gaggaggctt tcaccggctg gcgccattcg cccgagcaca acgccaatct
     1741 gctcatgccg caagcgaccc gcttcggcat cgccatcgcc aaggacccgc gcacgcatct
     1801 gcgcgtctac tgggcgatgg agcttgcggc ggagcccgaa aagccggcgc gcggcgaagc
     1861 cgcggggctg atctcgacgc tgggcggagc ggtcgtcgcg aatccgtaaa cgcgaactgc
     1921 tcggtcgtca tagcaatcca ttcgtcgggc gcggcctatg aatggattgc ttcgtcgctt
     1981 cgctcctcgc aatgatggat cgtgccgcga ggtcaccccg tcgcgccggc ctccgtcgcc
     2041 cgcctcgcct tgccgccggc gccggcgatc acgacgacga acaggaacac gccacggatc
     2101 accgactgcc aataggcgct gaggctgatc cagcccatgc cgttctcgaa attcaggatg
     2161 ttgaagacca ggccgagcag cagcacgccg gccagcgtcg cgccgaccga gccgcggccg
     2221 cccgaaagca gcgtgccgcc gaccacgacc gccgcgatcg agaagagctc ccatccgacg
     2281 ccttcggttg gctggccggc gccgaattgg gcggcgagaa tgacgccggc gaggctcgcc
     2341 agaagcccag attgtacgta ggtgaaaaac tcgacccgct cgaccggcag tcccatcagc
     2401 cgcgcggcgt cgggctcgcc gccgaccgcc aaagcgtagc gaccgaaggg cgagtagttg
     2461 agcaggatcc agccgacgac ataggcaagg atcgcaatcc aggcggggat cggaaagccg
     2521 agaaagtcgc cttggccgat cgcggtgaac gcggtgtcgt aggagacgcc gacggtctga
     2581 ttatgcgccg cgagaagcgc gctgccgccc gccatcaacg tgcccgccag cgtggcgatg
     2641 aacggcaaaa tcttgagccg cgtcaccatc gcgccattga tcgcgccgat aatgagaccc
     2701 gagccgaggc cgccggcaag gccggcgatc agcccatagg gcgacagaat cgccgagatc
     2761 acgctggcga gcgccgccac cgcgccgacc gaaagatcga tgccgccggt gatgatgacg
     2821 aagcacatgc cgagcccggt cagcgcgaac atcgaattgt agcgcaacac tgaaaagacg
     2881 ttgtagagcc cgaggaaatg atcgtagcgc agcgcgccga aaaggatcag cgcggcgagc
     2941 gcgaccaagg cgcggttgct gctcagaaag gcggtgaagc gattgcgcgg cgtcggctcg
     3001 acgacgatcg gcggcggcgt cacggcgtct tgtccgcgcc gctgcagcca gacggcgccg
     3061 gcgatgatcg cgcctttgac gacgagcgcg gcggcgtccg gcacgccatt ggcgagcagc
     3121 gtgtagcgca gcaattggat gatcagcgcg ccgagcagcg tgccgccgac attggcctgg
     3181 ccgccggtga gcaacgtgcc gccgaccgcg acggcggcga tcgcgtcgag ctccatgccg
     3241 atgccgacga ggtttgcgtc gctcgacgaa ttgatcgcga cgacgatgag accggcgatc
     3301 ccggcgcaaa gcccgctgat cacatagacg cggcgcttga cccggtcgac cggcatgccg
     3361 ccgagccgcg ccgccgcctc gttgccgccg accgcgagaa tctggcgccc gaacagcgtg
     3421 tggcgcagca tccaggcggc cgccgccacc agagccagca tcagcaggac ctgaaacggc
     3481 gcgccgaaaa tccggccgag gccgatgaat tggaaagtcg ggttcttgaa aacctgcaaa
     3541 ttgccgttgg tcatcacctg gccgatgccg cgcccggcga tgaacaggac gagcgtgccg
     3601 acgatcggct ggacgcgaaa gcgcgtgatg aaccagccgt tgaacaagcc gaataggccg
     3661 gtgacgacga ccggcgcgac gaaagcgcag gcgacgccga gggcgggatg ggcgacgccg
     3721 aacgggctga ggaaaatgat cggcgccagc gcgccggaaa tcgccatcag cgagccgacc
     3781 gagagatcga tgccgccggt ggcgatgacc agcgtcatgc cgaccgcgac gatgacgatg
     3841 gtcgcgactt gagtgagatt gacgttgagg gtctgccagg aaacgaaatg cggcgtgaaa
     3901 gcgagattga acaggatcag cagcagaagc ccggcgcccg cgccgtagcg ccgcgcccag
     3961 gcggcggcgt tgaagcgggg gctgagcgcg ggggtgatcg atacgctagt caagcttctt
     4021 ctcggcgatg acgccgtcct cgctgccgcc atgcgccatg acggccatga tgttcggttc
     4081 gctgatcgag tctcccgaca gctccgcagc ggtctcgccg tcgcgaagca cgaagacgcg
     4141 gtcggcgcct tcgacgattt cctcgagctc tgaagagatc atcagcacgc cgagcccctc
     4201 ggcggcgagt tcgtcgatta gggactgaat ctcgcccttg gcgccgacat cgatgccgcg
     4261 cgtcggctcg tccaggatca gcagcttggg attggtgcaa agccagcgcg cgagcaggac
     4321 cttctgctgg ttgccgccgg agagctccct gattttctga tcggcgctcg ccgccttaac
     4381 gccgagccgc gccatgaact tggcgacgac ctcgcgctgc ttggcctcgt cgacgacgcc
     4441 ggcgcgcgtc agccgcggca gaagcgccaa ggtcaggttc tcgccgaccg acatattggg
     4501 gatgacgccg tcgcgcttgc ggtcctccga gcaatagccg agaccacgcc ggatcgcctc
     4561 gatcggctcg ccgagcgagg ccttttcgcc cgcgatgtcg atccggccgg cgtccggcgg
     4621 gtcggcgccg aaaatcgcgc gggcgacttc cgtgcggccg gagccgagca ggccggcgag
     4681 cccgacgatc tcgccggcgc gaaccgcaag gcgcgcgttt cgcacccgcc ggccgatttt
     4741 aagcccttcg gcgcgcaaga tttccgcgcc gaccttggcg cggccgcggc tgaacgcggt
     4801 ggcgcccttg gcgcgcaccg cggcaaggtc gcggccgagc atcgccgcga cgagctgcag
     4861 cttgtcgaga ttggcgagcc ggtcgacgag cacggtccgt ccgtcccgca tcacggtgac
     4921 gcgttcacag acctgataaa gctcgtcgag ccggtggctg acgaaaatca ccgacacgcc
     4981 atcctgcttg agccgccgga tcacgtcgaa cagaacctcg cattcgcggt cgtcgagcga
     5041 ggaggtcggc tcgtccatga tgacgaggcg cgccgagtag gagacggcgc gggcgatggc
     5101 gaccatttgc tgaatcgcgg tcgagaaggt catcaagggg cggcggacgt cgatctcgac
     5161 cgagaagcgg cggaggatgt ccgcggcctc ggcgttcatc cgccgccaat cgatcatgcc
     5221 gtagcggcgc ggctccttgc cgaggcagat attttcggcc gccgagcgat aggggatgag
     5281 attgatctcc tggtagatcg tcgcgatccc gccgcgctgg gcttgctggg gcgagccgaa
     5341 ctcgaccggc cgcccggcga aatggatcgt acccgcgtcc ttgcgatgaa cgccggtcag
     5401 cactttgatc agcgtcgatt tgccagcccc gttttgaccg acaagggcca tgccttcgcc
     5461 gggcgcgaca tcgatgcggg cgccggccag cgccttggac ccgccaaagg ccttttcgat
     5521 tccctccatc gaaagaatcg gagccggaga agcggcgtcg atcgccgaca tgaaacgcgc
     5581 gcccttttta gaaataaaaa ctccctcgcc cggccgggcg agggagggct tttctcatca
     5641 aaccacaatc agaacgcgct cgccaagacc tctttggcgt tgctcgaatc gaagaagcga
     5701 tcctcgttct tgatataggg cggaatggtc ttgcccgccg catagtcttt catcgtctgg
     5761 aaggcctttg gcccgaagcg cggattgcac tcgcaagtgc agccgagctt gccgtcgatg
     5821 atcgcctgga cggcgtcctt ctcgccgtcg atcgacacga cgagaacgtc cttgccgggc
     5881 ttcttgccag ccgcttcgag cgcggcgatg gcgccgagcg ccatctcgtc gttatgggcg
     5941 tagatcgccg tcacgtcggg atgagcctgc agcaacgtct cggccacctg ccggcccttg
     6001 tcgcgggcga aatcgccgct ctggctggcg aggatgacca tgcccggcca tttcttgatc
     6061 gcttcgtcga agcccttctt gcgatcgttg gccggcgagg agccggtcgt gccttcaagc
     6121 tcgatgatct tggccttgcc gtcgaccgcc ttggcgagcc aggcggcgac gcgctggcct
     6181 tcctcgacga aattcgagcc gatgaaggtg acataatcga cgccgggctt ggccatggaa
     6241 tgatcgacgt cgcgatcgat cagaaagacc ggaatgccgg ctttcgcggc cgccttgatg
     6301 accggcacca acggcttgtc ctcgcgcggc ggcaggaaga tggcgtcgac gccttgcgcg
     6361 atcatgctgt tcacgtcgga ggcctgcttg gcggccgagc cggcggcgtc cgtatagacg
     6421 agctgatagc cgagcttctt ggcctcgtcc tgcatgctcg ccgtctcggc gaggcgccac
     6481 ggattgttgc tttccgtctg cgcgaatccg accttgtacg tggccttctg cttgagcgcc
     6541 ggcgcggccc atgcagagcc catcgccaag ccggccgcgc cggcggccga cgtgatgagc
     6601 aatctgcgac gcgtgatgat catcgtgaca cctccctaaa aggactgctg tcgcggtctt
     6661 ctcgccgcct cgcgggcggc gccgcgcgag ttcgcggccg cttgcgaaga atgttccact
     6721 gctgctaata attgtcaatc gcaaacatcg ctttccatcc ttctgcgact cagaatttcg
     6781 atattccaat agtttaggtc tgctaatatg cgcataatgg cgcggtgagg aaacggcgat
     6841 gtccgaacgg cccggcgcaa tcgcggatat cggccagccc ggcaaggccc cgctcgggcc
     6901 cgatgcggcg gcccgccgcc gcccgaccat gatcgaggtc gcggcggagg ccggcgtctc
     6961 gcagacgacc gtttcgctgg tcctgaacga cgcgcaaggg gcgcggcttt cgacagaaac
     7021 gcgcgagcgc gtgttcgagg cggcgcgccg cctcggctac cgattggccc ggcgcaacgc
     7081 gccggcgctc gccggcgcgg cgacgatcgg cttcgtcgtc aatgagttgt cgaccgatcc
     7141 gtggacggcg atcgccatgg acggcgcccg cgacaaggcc tgggagcatg gcctgacgat
     7201 cagcgcggcg gtgacgcgcg gcgacgccga gatggagcag gcggtgctcg cccagatgac
     7261 ggcgcagccc atgctcggtt tgatctatgg cacgatcaac acccggcgca tcacgccgcc
     7321 gcgggcgctc tatcgcgtgc cgacggtact gctcaattgc tatgtcgccg accgctcgct
     7381 gccctcggtg gttccgagcg aagtcgccgg cggccatgtc gcgacgcgac gcctcatccg
     7441 ggcgggccat cgccgcatcg gctatatcaa cggagaagcc ttgatggacg cctgccgcga
     7501 ccgcctgaaa ggctaccgcc aggcgctggc cgccgccgac ctgccctacg atcccgatct
     7561 cgttcgccca ggcaactggg agccctccgc agggtacgag cagaccttcg ccctgatgcg
     7621 gctcgccgag ccgccgaccg cgatcttctg cgccaacgat ctcatggcgg tcggctgcat
     7681 cgaagcgctc aaggagatgg gccggcgcgt gcccgaggac gtcgcggtcg tcggctatga
     7741 cgatcgcgag atcgcccagt tcacgcatcc gccgctgacc accgttcttt tgccccatta
     7801 cgagatgggc gcggaagccg ccgcctatct catcgattac gcgcgccgcc cgcccgcccg
     7861 gccgccgcaa atcaaggtcg agtgtccgct gatcgagcgc aagtcggtcg cgccgggcgg
     7921 cggcgaggct tagcgtgatt tctcacggtg gggcgcattg gacgccgacg gagcatagcc
     7981 cgtttgtgat ccgtttagag gttggcgacg atcaccgagg caggcggtga agaaaagatg
     8041 cactggagcc gtgatcccaa atttttccaa aacttttctt ggccgttt
//
LOCUS       DATGJW010000100         3603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_951706, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000100 DATGJW010000000
VERSION     DATGJW010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3603
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_951706"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..1823)
                     /locus_tag="VKU03_02955"
     CDS             complement(207..1823)
                     /locus_tag="VKU03_02955"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007702388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLJ70248.1"
                     /translation="MGEAFIGVDVGTGSARAGVFDGDGRLLASARKPIAIWREAGEIV
                     EHSSQDIWRAATGAVRDAVHGAGLSAEDVKGVSFDATCSLAVLDPNGASLPVGPSGDP
                     NRDVIVWMDHRAVGEADFINKSGDDVLRYVGGKISPEMQAPKLLWLARNRPDTFAQAG
                     HFLDLTDFLTWRASGATARSVCTVTCKWTYLAHERRWSREFFDKIGLSALCGNSFARI
                     GAEMVEPGAPLGAGLTQEAADAMGLLPGIPVGAGLIDAHAGALGTLAASRAGEKADPR
                     RRLALILGTSACCMAVADEARFVDGVWGPYFSALTPGQWLAEGGQSAFGAAIDRLMEA
                     HPAFGAVKSRAGDRAFEEMENEILARAGGLSRAALLAKDLHVLPDFIGNRSPYADPAA
                     RGAVIGLDLRADHQSLLELYVAALVGLAHGVGQIIGTLEAGGYDFDMLVVSGGAARSK
                     LVRQLIADATQKKVGAPETSEPVLLGAAMLAAVAAGRHTVASAMSAMSRLAEEIAPQK
                     GEIAAFHARKGQAFRLTQDIERQVRAIMSA"
     gene            complement(1929..2924)
                     /locus_tag="VKU03_02960"
     CDS             complement(1929..2924)
                     /locus_tag="VKU03_02960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HLJ70249.1"
                     /translation="MTAPVSSLTEHIRPIAAAAPVTAAAFIGGAPALALGDGAVLIGD
                     PGAPKRVDAHPEGAILVAASDGERLIAGGDDGRVVAVSPDGSSEQIADEKGRWIDALA
                     SKGGAIAWAAGRDARVRDAAGAIKSYAAPSTVRGLAFLPKGYRLALAHYNGVSLWFPN
                     AAAAPTLLEWKGSHLDVTASPDGRFIVSSMQEDALHGWRVADSRNMRMTGYPKKTRSL
                     SWSHDGHWLATSGADACVVWPFRDKDGPMGKAPRETGVRQAKVSQVAFHPRALVVAIG
                     YEDGFILLCRLGDAAEIFVRGPGDGAVTALAWDASGARLLFGLDSGAAGLLTLPA"
     gene            complement(3182..>3603)
                     /locus_tag="VKU03_02965"
     CDS             complement(3182..>3603)
                     /locus_tag="VKU03_02965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLJ70250.1"
                     /translation="EPDFLETGDYHDHDHDHHHDHDGHGHDHGHGHGGLKQHYHDEHM
                     QSVSVKLDGDVDPGKFMPWINELTQIQGPNILRCKGIVAFSGDPKRFVFQGVHMMLDG
                     ELQREWKAGEKRESKVVFIGRDLKEDQIREGFLACAA"
BASE COUNT          579 a         1211 c         1239 g          574 t
ORIGIN      
        1 ccgtcgcaat cgaaaatgac gacctcaggc caggccatgg cgcctgcttc gcgtttcctc
       61 gcgcaatggg cgcgcttgcc gcgcgcttcg aacctttgcg ccggccggca ggctatcacg
      121 cggcgcgcca atggcgcaag caaatgccca ttcttggggt aattgctcag catcccgcga
      181 gcaaggcggc gtcccggcgc gcatcgtcag gcgctcatga tcgctctgac ctgccgctct
      241 atgtcttggg tcaggcgaaa agcctgacct ttgcgggcgt ggaaagccgc gatttcgcct
      301 ttctgcggcg cgatctcctc ggcgaggcgc gacatcgccg acattgcgga ggccacggtg
      361 tggcggccgg cggcgaccgc ggcgagcatc gccgcgccga gcaaaaccgg ctcgcttgtc
      421 tccggcgcgc cgaccttttt ttgcgtcgcg tcggcgatca attggcgcac cagcttgctg
      481 cgcgccgcgc cgccgctgac gacgagcatg tcgaaatcat agccgccggc ttcgagcgtg
      541 ccgatgatct ggccgacgcc atgggcgagg ccgaccaagg cggcgacgta gagttcgagc
      601 aggctctgat ggtcggcgcg caggtcgagg ccgatcaccg cgccgcgcgc ggccggatcg
      661 gcgtaaggcg agcggttgcc gatgaagtcg ggcagaacat gcaggtcctt ggcgagcagg
      721 gcggcgcgcg acaggccgcc ggcgcgggcc aagatttcgt tctccatctc ctcgaaagcc
      781 ctgtcgccgg cgcggctctt gaccgcgccg aaggccggat gggcctccat cagccggtcg
      841 atcgccgcgc cgaaggccga ctggccgccc tcggccagcc attgccccgg cgtcagcgcg
      901 gaaaagtacg ggccccagac gccgtcgacg aagcgcgctt cgtcggcgac cgccatgcag
      961 caggccgagg tgccgaggat cagcgcgagc cggcggcgcg ggtcggcctt ctcgccggcg
     1021 cggctcgccg ccaacgtgcc caaagcgccg gcatgggcgt cgataaggcc ggcgccgacc
     1081 gggatgcctg ggagaagccc catcgcgtcg gccgcttctt gcgtcaggcc cgcgccgagc
     1141 ggcgcgccgg gctcaaccat ttcggcgccg atgcgggcga agctgttgcc gcacagagcc
     1201 gagaggccga ttttgtcgaa aaactcgcgc gaccagcgcc gctcatgggc gaggtaggtc
     1261 catttgcatg tgacggtgca gacggagcgg gccgtcgcgc ccgaggcgcg ccaggtgaga
     1321 aaatccgtca gatcgaggaa atggccggct tgcgcgaacg tgtccggccg gttgcgggcg
     1381 agccagagaa gcttcggcgc ctgcatttcc ggggagattt tgccgccgac atagcgcaga
     1441 acgtcgtcgc cgcttttgtt gatgaaatcg gcctcgccga ccgcgcgatg atccatccag
     1501 acgatgacgt cgcggttggg atcgccgctc gggccgaccg gcagcgaggc gccgttggga
     1561 tcgagaacgg cgagcgagca ggtggcgtcg aagctgacgc ctttgacgtc ctcggccgaa
     1621 agcccggcgc cgtggacggc gtcgcgaacg gcgccggtcg ccgcgcgcca aatgtcctgc
     1681 gatgaatgct cgacgatctc cccggcttcg cgccagatcg cgatcggctt gcgggcgctg
     1741 gcgaggagcc ggccgtcgcc atcgaacacg ccggcccgcg cgctgccggt gccgacatcg
     1801 acgccgataa aagcttcccc catgcgcgcc ctcgccctcg tgcagggcgc acattcccat
     1861 cacgaggcgc ggtttggcaa ggcgcgcaac ccctcctccc cgccttgcga ggagaaggga
     1921 gacggcgctt aagccggcag cgtcagcagg ccggcggcgc cgctgtcgag tccgaagagg
     1981 aggcgggcgc cgcttgcgtc ccaggcgagg gcggtcaccg cgccgtcgcc ggggccgcgc
     2041 acaaaaatct cagccgcgtc gccgagccgg cagagcagga tgaacccatc ctcatagccg
     2101 atcgcgacga cgagcgcgcg cggatggaac gcgacttggc tgaccttggc ctgccgtacc
     2161 ccggtctcgc gcggcgcctt gcccatcggg ccgtccttgt cgcggaacgg ccagacaacg
     2221 caagcgtcgg cgccggatgt cgcgagccaa tgtccgtcat gcgaccagga tagggagcgc
     2281 gtctttttcg ggtagccggt catgcgcatg ttgcgcgaat cggcgacgcg ccagccatgc
     2341 agcgcgtcct cctgcatcga ggagacgatg aagcggccgt ccggcgaggc ggtcacgtcg
     2401 agatgcgagc ctttccattc gagcaaggtc ggcgcggcgg cggcgttggg gaaccacaaa
     2461 gataccccat tgtaatgagc gagggcgagg cgatagcctt tcggcagaaa agcgaggcct
     2521 cgtactgtcg acggcgcggc gtagctcttg atcgcgcctg cggcgtcgcg aacgcgcgcg
     2581 tcgcgtccgg cggcccacgc gatcgcgccg cccttgctcg ccagcgcgtc gatccagcgg
     2641 cctttttcgt cagcgatttg ctcgctcgat ccatcgggcg agaccgcaac gacccggccg
     2701 tcgtcgccgc cggcaatcag ccgctcgccg tcgcttgccg cgacgagaat cgcgccctcg
     2761 ggatgcgcgt cgacgcgctt cggcgcgccc gggtcgccga tcagcacggc gccgtcgccg
     2821 agcgccaaag ccggcgcgcc gccgatgaag gcggcggccg tcaccggcgc ggcggcggcg
     2881 atcggccgga tatgctcggt caacgaagag acgggcgcgg tcattggctt tccaggcgtg
     2941 gcgagacgag gatgctagct tgtactcatc ctacgaagag cgggcgaacc gacgaggcag
     3001 aatgagcctc ggcgtcgggg acacctctcc ccgtggagcg aagcggaacg gggagaggta
     3061 cggagagggg ctggttccgt ttgccccgac attggagcct tattcccccg cccctctcag
     3121 tacctcttcc cgcttcgcgg ggaaaggaga cttccgcgtc gcgcttcgtt ctcaggcgcc
     3181 tctacgccgc gcaggcgagg aagccttctc tgatctgatc ctctttcaga tcgcggccga
     3241 tgaagacgac tttcgactcg cgcttttcgc ccgccttcca ttcgcgctgc agctcgccgt
     3301 ccagcatcat atgcacgccc tggaagacga agcgcttggg atcgccggaa aaggcgacga
     3361 tgcccttgca gcgcaggatg ttcgggccct gtatctgagt gagctcgttg atccacggca
     3421 tgaacttgcc gggatcgaca tcgccgtcga gcttgaccga caccgattgc atgtgctcgt
     3481 cgtgatagtg ctgcttcagc ccgccatgac catgaccatg atcatggcca tggccatcgt
     3541 ggtcgtgatg atgatcgtgg tcgtggtcgt gatagtcgcc ggtctccagg aaatccggct
     3601 cga
//
LOCUS       DATGJW010000101         4025 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_225941, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000101 DATGJW010000000
VERSION     DATGJW010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4025)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4025)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4025
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_225941"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..800
                     /locus_tag="VKU03_02970"
     CDS             <1..800
                     /locus_tag="VKU03_02970"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006206 - pyrimidine nucleobase metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HLJ70251.1"
                     /translation="ESIPLITASILSKKLAAGLQGLVMDVKTGSGAFMASLAGARDLA
                     KSLADVATGAGLPTDALITDMNEPLASAAGNAVEVTNAVNYLTGARRDPRLHAVVKAL
                     GGQLLARGGLAKDAAAGEAAMEASLAGGKAAERFERMVATLGGPRDFLANAERHLPRA
                     PVVIAAPPARRGFVERIDVRAVGMAVVALGGGRARAADAIDPAVGFTALAGIGAEVGP
                     DAPLALVHARSDAQAAAAAAALAAAYRIGDAAPAAHPPVVERITAAP"
     gene            797..1744
                     /locus_tag="VKU03_02975"
     CDS             797..1744
                     /locus_tag="VKU03_02975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007872925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HLJ70252.1"
                     /translation="MTILLAIEPASEIPPWRERFAALLPGRRLTDAPKAGERAEISYA
                     LSWRHRAGALADLPNLKAIFSLGAGVDHLFKDPLLPDAPIVRVVDDDLTKRMSEWVVL
                     HALLHLRQQRLYDLRQAERHWEEDLNQPAAKDVRIGLMGLGALGRDAARKLSVIGFDV
                     AGWSRGEKRIDGVACFHGAAGLDAMLARTDILVCLLPLTAETRGVLNARLFAKLARDG
                     RLGGPILLNAGRGGLQVEADILAALDSGLLKAASLDVFEREPLPKESPLWRHPRVYVS
                     PHSAALSEPGAVARNIAAQIEAYERGEPLKNVVDRTRGY"
     gene            complement(1896..2882)
                     /gene="araD1"
                     /locus_tag="VKU03_02980"
     CDS             complement(1896..2882)
                     /gene="araD1"
                     /locus_tag="VKU03_02980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraD1 family protein"
                     /protein_id="HLJ70253.1"
                     /translation="MRLAQIIDEAGKRALVVTARGESRLVKGARSTYDLAMQAIEAGE
                     SLRKLIAARGVGKPVDLAKALKEKRVLNPIDHKDPAHLLVTGTGLTHLGSAEGRDKMH
                     KDAADPAKQTDSMRMFRMGLEGGKPQGSAAGVQPEWFYKGDGSTVAAPGGDLVSPDFA
                     LDGGEEPEIVGLYIVAPDGAPVRVGFALGNEFSDHVTERQNYLYLAHSKLRASSFGPE
                     LLVGDLPPDVQGKSRILRGKEKLWEKPFISGEQNMSHSIANLEAHHFKYDLFRRPGDV
                     HVHFFGTATLSFSDGIKTQKGDVFEIEAEPFGLPLRNRLAIAKAKRVKVKAL"
     gene            complement(3121..>4025)
                     /locus_tag="VKU03_02985"
     CDS             complement(3121..>4025)
                     /locus_tag="VKU03_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HLJ70254.1"
                     /translation="FQIGRMKEEYGIVEGDLFQTMTIQATGGTKKTIEAGLQRIAAML
                     PIVNRAERETIPASELLLALQCGGSDGYSGITANPALGVAVDILVRHGGAAILSETPE
                     IYGAEHLLTRRAATREIGEKLVELIHWWEEYTARNHGEMNNNPSPGNKAGGLTTILEK
                     SLGAAAKGGGTILNGVYRYAEPVTAKGFVFMDTPGYDPVSATGQVAGGANILAFTTGR
                     GSAYGCKPTPSIKLATNSDTYRRMVDDMDINCGDILDGVGLEEKGQEIFEKLLAVASG
                     ERSKSEALGYGDAEFVPWQVGATM"
BASE COUNT          593 a         1364 c         1392 g          676 t
ORIGIN      
        1 tcgaatcgat cccgctgatc accgcctcga tcctgtcgaa gaagctcgcc gccggcctgc
       61 agggcctcgt catggacgtg aagaccgggt ccggcgcttt catggcgagc ctggccggcg
      121 cccgcgatct cgccaagagc ctcgccgacg tcgcgaccgg cgcaggcctg ccgaccgacg
      181 cgctgatcac cgacatgaac gagccgctcg ccagcgccgc cggcaacgcc gtcgaagtga
      241 cgaacgccgt gaactacctc accggcgcgc ggcgcgatcc gcgcctgcac gccgtcgtca
      301 aggcgctggg cggccaattg ctggcgcggg gcggcctcgc gaaggacgcg gccgccggcg
      361 aagcggcgat ggaggcgagt cttgccggcg gcaaggcggc ggagcgcttc gagcgcatgg
      421 tcgcgacgct gggcggcccg cgcgatttcc tcgccaacgc cgagcgtcat ctgccgcgcg
      481 cgccggtcgt gatcgccgcg ccgccggccc ggcgcggctt cgtcgagcgc atcgacgtcc
      541 gcgccgtcgg catggcggtc gtcgcgctgg gcggcggccg ggcccgcgcc gccgatgcga
      601 tcgatccggc cgtcggcttc accgcgcttg ccggaatcgg cgcggaggtc gggccggacg
      661 cgccgctggc gctcgttcat gcgcgcagcg acgcgcaagc ggcggcggcc gcggcggctt
      721 tggccgcggc gtaccggatc ggcgacgccg ctccggcggc gcatccgccg gtcgtcgaac
      781 gcataaccgc cgcgccgtga ccattcttct ggcaatcgag ccggcgagcg agatcccgcc
      841 ttggcgcgag cgcttcgcgg cgctgctgcc ggggcggcgc ctgactgacg cgccgaaagc
      901 cggcgagcgc gccgagattt cctacgcgct gagctggcgc catcgcgctg gcgcattggc
      961 cgatctgccc aatctcaagg cgatcttctc gctcggcgcc ggcgtcgatc atttgtttaa
     1021 ggatccgctt cttcccgacg cgccgatcgt gcgggtggtc gacgacgact tgaccaaaag
     1081 gatgagcgaa tgggtcgtgc tgcatgcgct cctgcatctg cgccagcagc ggctttacga
     1141 tctgcgccag gccgagcgcc attgggagga ggacctcaac cagcccgcgg cgaaggacgt
     1201 gcggatcggc ttgatgggcc tcggcgcgct cggccgcgac gcggcgcgga agctcagtgt
     1261 gatcggcttc gacgtggccg gctggagccg cggcgaaaag cgaatcgacg gcgtcgcttg
     1321 ctttcacggc gccgcggggc tcgatgcgat gctggcgcgc acggacatcc tcgtctgcct
     1381 gctgccgctg accgcagaga cgcgcggcgt tctcaatgcg cgcctcttcg cgaagcttgc
     1441 tcgcgacggc cggctcggcg gtccgatcct ccttaacgcc ggccgcggcg gcttgcaggt
     1501 cgaggccgat attctcgccg cgctcgattc cggcctgctc aaagcggcct cgctcgacgt
     1561 gttcgagcgc gagccgctgc cgaaagaatc gccgctctgg cgccatccgc gcgtctatgt
     1621 cagcccgcac agcgccgccc tctccgagcc cggcgccgtc gcccgcaaca tcgccgccca
     1681 gatcgaggca tacgagcgcg gcgaaccgct gaaaaacgtc gtcgatcgca cgcgagggta
     1741 ctaatcctcc cccgctccgc gaaggcggaa cgggggaggg ggaccatgcg aagcatggtg
     1801 gagggggtgg cggcggttca ggataatcct ctggcgccga agcccccccg cgctgacgcg
     1861 cggtccccct cccccgtctt cgacggggga ggaattcaca gcgccttcac cttcacccgc
     1921 ttcgccttgg cgatcgccag ccggttgcgc agcggcaggc cgaagggctc ggcctcgatc
     1981 tcgaacacgt cgcccttctg ggtcttgatc ccgtcggaga aggagagcgt cgcggtgccg
     2041 aagaagtgga catgcacgtc gccgggccgg cggaagagat cgtatttgaa atgatgcgcc
     2101 tcgagattgg cgatcgaatg cgacatgttc tgctcgcccg agatgaacgg cttctcccac
     2161 agcttttcct tgccgcgcag gatgcgcgac tttccttgca cgtcgggcgg aaggtcgccg
     2221 accagcaatt cggggccgaa ggacgacgcg cgcagcttgg aatgggcgag gtacagatag
     2281 ttctgccgct cggtgacgtg gtcggagaac tcgttgccga gcgcgaagcc gacgcggacc
     2341 ggcgcgccgt ccggcgcgac gatatagagg ccgacgattt ccggctcctc gccgccgtct
     2401 aaggcgaaat cgggcgagac gagatcgccg cccggcgcgg cgacggtcga gccgtcgccc
     2461 ttgtagaacc attccggctg cacgccggcc gccgagccct gcggtttgcc gccttcgaga
     2521 cccatccgga acatccgcat cgaatcggtc tgcttggccg gatcggcggc gtccttgtgc
     2581 atcttgtcgc ggccctccgc cgagccgaga tgggtgaggc cggtgccggt gacgaggaga
     2641 tgggcggggt ccttatggtc gatcgggttc agcacgcgct tttccttgag cgctttggcg
     2701 agatcgacgg gcttgccgac gccgcgcgcg gcgatcagct tgcgcaagga ttcgccggcc
     2761 tcgatcgcct gcatggcgag gtcgtaagtg ctgcgcgcgc ccttgacgag gcggctctcg
     2821 ccgcgcgccg tgacgaccag ggcgcgcttt ccggcttcat cgatgatctg cgcgagacgc
     2881 atgagctttc ccctctaata ttgccgcgat tttatcgcgc atcgaaagca aagaatgtac
     2941 gaagccgcaa ggggaaaatc cccccgcccc gcaaagcggg gagcgggtgg ccgcgaagcg
     3001 gccggttgag gggctgcgcc gagcctgccg attagctttc gtacggcgcg tacctttcga
     3061 gtcctgcgcc gcccgtcatc cggcgcttcg cgctgcgttc tccccgcttt gcggagctcc
     3121 tcacatcgtc gcgccgacct gccagggcac gaattcggcg tcgccatagc cgagcgcttc
     3181 ggatttcgag cgctcgcccg aggcgaccgc cagaagcttt tcgaaaatct cctgcccctt
     3241 ctcctccagc ccgacgccgt cgagaatgtc gccgcaattg atgtccatgt cgtcgaccat
     3301 gcggcgatag gtgtcggaat tggtggcgag cttgatcgag ggcgtcggct tgcagccata
     3361 ggctgagccg cggccggtgg tgaaggcgag gatattggcg ccgcccgcca cctggccggt
     3421 cgccgagacc ggatcgtagc cgggcgtatc catgaacacg aagcccttgg cggtcaccgg
     3481 ctcggcgtag cgatagacgc cgttgaggat cgtgccgccg cccttggccg cggcgccgag
     3541 cgatttttcg aggatcgtcg tcaggccgcc ggctttgttg ccgggcgaag gattgttgtt
     3601 catctcgccg tgattgcgcg cggtgtactc ctcccaccag tgaatgagct cgacgagctt
     3661 ctcgccgatc tcgcgcgtcg cggcgcggcg ggtgagcaga tgctcggcgc cgtaaatttc
     3721 cggcgtttcg gagaggatcg cggcgccgcc atggcggacg aggatgtcga cggcgacgcc
     3781 gagcgccgga ttggcggtga tgcccgaata gccgtccgag ccgccgcatt gcagcgcgag
     3841 gagcaattcc gaggcgggga tcgtctcgcg ctcggcgcga ttgacgatcg gcagcatcgc
     3901 ggcgatgcgc tgaagcccgg cttcgatcgt cttcttggtg ccgccggtcg cctggatggt
     3961 catggtctgg aagagatcgc cctcgacgat tccgtattcc tccttcatcc ggccgatctg
     4021 gaaca
//
LOCUS       DATGJW010000102         4007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_432718, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000102 DATGJW010000000
VERSION     DATGJW010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4007
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_432718"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1070)
                     /locus_tag="VKU03_02990"
     CDS             complement(<1..1070)
                     /locus_tag="VKU03_02990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLJ70255.1"
                     /translation="MNQTSAKDYSQTLFLPKTDFPMRAGLPQKEPEILARWAREDLYG
                     RLRAAAKGRAKFVLHDGPPYANGNIHIGHALNKILKDMVVRSQQMAGKDSNYVPGWDC
                     HGLPIEWKVEEEHYRAKGKAKPDFSDPAAIVAFRKECRAYAEHWINVQREEFKRLGVV
                     GDWDHPYLTMTYPAEAQIAREIMTFAREGLLYRGSKPVMWSVVEKTALAEAEVEYEDY
                     VSDTVYVAFPISAIGTSAPADHGLGALRHAEFVANLKKASVVIWTTTPWTIPGNRAIS
                     FSEKIAYGLYQVTAAPAGNWAKTGANYVLAKNLAEETFKAAKVESYKLLGDVGPTFLL
                     DAICAHPFAANGYAFDVPLLPG"
     gene            complement(1243..1479)
                     /locus_tag="VKU03_02995"
     CDS             complement(1243..1479)
                     /locus_tag="VKU03_02995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF433 domain-containing protein"
                     /protein_id="HLJ70256.1"
                     /translation="MHDFPRITQDPAVMGGKPCIRRLRVTVGMIVGQIGAGRSIQELL
                     ADYPYLERQDVFEALRYAAWRAEEREVELRRPPC"
     gene            complement(1510..2505)
                     /locus_tag="VKU03_03000"
     CDS             complement(1510..2505)
                     /locus_tag="VKU03_03000"
                     /EC_number="2.7.1.26"
                     /EC_number="2.7.7.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003919 - FMN adenylyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional riboflavin kinase/FAD synthetase"
                     /protein_id="HLJ70257.1"
                     /translation="MSPGRAENIILANDPLKAPAGLENGVYAIGNFDGVHRGHAAVIG
                     RARRLADELGAPAAALTFEPHPADFFAGRPVVFRLTPPPLKARALAELGVDGVVTLTF
                     NAALAGLTAEAFVSTILVGRLGIGAAVIGGDFHFGKNRGGSPEFLVEAGRRHGFRVDV
                     IEKVADSGGEAAISSTAIRRALEAGDVETAAGLLGRPYCVEGEVIAGRKLGRTLGLPT
                     ANIALAPTNRLAHGVYAVRATAAGKRFDGVASFGTRPTVEGAGAPLLEVFLFDFSGDL
                     YGKTMDVAFEAYLRPELKFESLEALKARMAEDVERARIALRSAPDRIGPASAARP"
     gene            complement(2656..3552)
                     /locus_tag="VKU03_03005"
     CDS             complement(2656..3552)
                     /locus_tag="VKU03_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HLJ70258.1"
                     /translation="MTDISSATAAPLIDGLRLLAGRYDVALCDVWGVIHNGRRAFPAA
                     CDALTRFRESGGTVVLITNAPRPNGPIQAQLDRLGVPRGAYDAIVTSGDVTVGLVRAR
                     GAAPLHHIGPARDLTLFDTLAELGAAAPRVALAEASYVLCTGLFDDTRETPADYDATF
                     AAMLARKLTMICANPDLVIHRGDDLVYCAGALAQRYADGGGETIYAGKPYAPIYHEAL
                     ARAAAARGGEIDKSRVVAIGDAMRTDIAGAASQGLDALFVAAGIHRDEIGGESAIDAA
                     SFEQFAARHGARPTAIIDTLVW"
     gene            complement(3600..>4007)
                     /locus_tag="VKU03_03010"
     CDS             complement(3600..>4007)
                     /locus_tag="VKU03_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70259.1"
                     /translation="RVLSFSSDRPEAALLSAYDRDILDKHAALGCRSLIVMAEGEAHP
                     FIFVKRKVMRGSIPCEQLIYCRDLGDFVAYAGALGRYLLARGGVVCITDANKPLPGLI
                     GRYLPDIGPKYFKGPAQPRLGDLTFTELTVLGP"
BASE COUNT          705 a         1328 c         1318 g          656 t
ORIGIN      
        1 tcgccgggga gcagcgggac atcgaaggcg taaccattcg cagcgaaagg atgagcgcat
       61 attgcgtcga gaagaaatgt cggccctaca tcgccgagca acttataaga ttcgaccttc
      121 gccgccttga acgtttcttc cgccaaattc ttcgcaagaa cgtaattcgc gccggtcttc
      181 gcccagttcc ctgcgggcgc ggccgttacc tgataaagac cataagcgat cttttcggag
      241 aacgagatcg cccgattgcc agggatcgtc cacggagtgg ttgtccagat aacaacgctt
      301 gctttcttca gattcgcaac gaattcggcg tgacgaagcg cgccgaggcc gtgatcggcc
      361 ggcgcggatg tcccgatcgc gctgatcgga aacgccacat aaaccgtatc gctgacataa
      421 tcctcgtact cgacctccgc ctcggccagc gccgtcttct cgacgacgct ccacatcacc
      481 ggcttcgagc cgcggtacag cagcccttcc cgcgcgaacg tcatgatctc gcgggcgatc
      541 tgcgcctcgg ccggataggt catcgtcaga tacggatgat cccagtcgcc gaccacgccg
      601 aggcgcttga actcctcgcg ctgcacgttg atccaatgct cggcataggc gcggcattcc
      661 ttgcggaagg cgacgatcgc ggccgggtcg gagaaatcgg gcttggcctt gcccttggcg
      721 cggtaatgct cctcctcgac cttccattcg atcggcaggc cgtggcagtc ccagccgggc
      781 acgtaattgg agtccttgcc cgccatttgc tgcgagcgca cgaccatgtc cttgaggatt
      841 ttgttgagcg cgtggccgat atggatgttg ccgttggcgt aaggcggccc gtcatgcagc
      901 acgaatttgg cccggccctt ggcggcggcg cgcaggcgcc catagaggtc ctcgcgcgcc
      961 cagcgcgcca gaatttccgg ctccttctgc ggcaggccgg cccgcatcgg gaaatcggtc
     1021 ttgggcagga agagcgtctg cgaataatct ttggcgctgg tctggttcat cgggcttgaa
     1081 agcgtctcgg gatcggcgtc gggcggcttg gccggacgga cgccgcgtcg tttaagcgat
     1141 ttggctggaa agcgccaatc gagtcggctc cgcctcgcgc cgagggccgg acctagatcg
     1201 catcgcccgg caattaaccc accgatcagg taaattgaag tctcagcagg gcgggcgcct
     1261 cagttcgacc tctcgctctt cagcgcgcca agcggcgtat cgcaaagcct cgaatacgtc
     1321 ctgacgctca agataagggt aatcggcgag caattcctgg atgctccggc cggcgccgat
     1381 ctgaccgacg atcatgccga ccgtcacgcg caagcggcga atgcatggct tgccgcccat
     1441 cacggcaggg tcttgggtga tgcggggaaa atcgtgcatc gttccaccct atctttcgcc
     1501 accatcgtat catgggcgcg ctgcggatgc ggggccgata cgatcgggcg cgctcctcaa
     1561 tgcaatccgc gccctctcga cgtcctccgc catccgcgcc ttgagcgcct cgaggctctc
     1621 gaacttcagc tccggccgca gataggcctc gaacgccacg tccatcgtct tgccgtagag
     1681 gtcgcccgaa aagtcgaaca gaaacacttc gagcagcggc gcgccggcgc cttcgacggt
     1741 cggccgcgtg ccgaaactgg cgacgccgtc gaagcgcttc cccgccgccg tcgcgcgcac
     1801 ggcgtagacg ccgtgcgcca gccgattggt cggggcgagc gcgatattgg cggtcggcag
     1861 gccgagggtt cggccgagct tgcggccggc gatcacctcg ccctcgacgc agtagggccg
     1921 gccgagcaag ccggccgccg tttcgacatc gccggcttcg agcgcgcgcc ggatcgcggt
     1981 ggatgaaatc gccgcctcgc cgccggaatc ggcgactttc tcgatcacat cgacgcgaaa
     2041 gccgtggcgc cggccggcct cgaccagaaa ttccggcgag ccgccgcgat tcttgccgaa
     2101 atgaaagtcg ccgccgatca ccgcggctcc aatgccgagg cgtccgacca aaatcgtcga
     2161 aacgaacgcc tcggccgtca ggccggccaa agccgcgttg aacgtcagcg tgacgacgcc
     2221 gtcgacgccg agttcggcca gggcccgcgc cttgagcggc ggcggcgtca gccggaacac
     2281 gaccggccgt ccggcgaaga aatcggccgg atgcggctcg aacgttagcg ccgcggccgg
     2341 cgcgccgagc tcgtcggcga gccgccgcgc ccggccgatc acggccgcat ggccgcgatg
     2401 gacgccgtcg aaattgccga tcgcgtagac gccgttttcg aggcctgccg gggctttcag
     2461 cggatcgttc gcaaggatga tgttctcggc gcggccgggc gacacgaaac gtccgttgat
     2521 ttgacagggt ctagggcgag tttgaaggcg gcgggaaact gccgcgcggc gccggcccgg
     2581 tcaagggcga aagctccgct gtcgatgccg tcgaccgcgt ccggccgcgc ccagcgagcg
     2641 cggcgacagg gcgccttacc agacaagcgt gtcgatgatc gcggtcggcc gcgcgccatg
     2701 ccgcgcggcg aattgctcga aagacgcggc gtcgatcgcg ctctcgccgc cgatctcgtc
     2761 gcggtgaatg ccggcggcga cgaacagcgc gtcgagccct tgcgacgccg cgccggcgat
     2821 atcggtgcgc atggcgtcgc cgatcgccac cacccggctc ttgtcgattt cgccgccgcg
     2881 cgccgccgcg gcgcgcgcca gcgcctcgtg atagatcggc gcatagggct tgccggcata
     2941 gatcgtttcg ccgccgccat cggcgtagcg ctgcgccagc gcgccggcgc aatagacgag
     3001 atcgtcgccg cggtgaatga ccagatcggg attggcgcag atcatcgtca gcttgcgcgc
     3061 cagcatcgcg gcgaacgtcg cgtcgtaatc ggccggcgtc tcgcgcgtgt cgtcgaaaag
     3121 gcccgtgcag aggacatagg aggcttcggc gagcgcgacc cgcggcgctg cagcgccaag
     3181 ctcggcgagc gtatcgaaca gggtcaagtc gcgcgccgga ccgatatggt gaagcggcgc
     3241 cgccccgcgc gcgcggacca gtccgacagt gacgtcgccg gacgtcacga tcgcgtcata
     3301 agctccgcgc ggaactccga ggcgatcgag ttgggcttgg atcgggccgt tcggccgcgg
     3361 cgcgttggtg atcagcacaa ccgtcccgcc gctctcgcga aatcgcgtca aggcgtcgca
     3421 ggccgcggga aaggcgcggc ggccgttatg aatcacgccc cagacgtcgc agagcgcgac
     3481 atcgtagcgg ccggccaaaa gccgcaggcc gtcgatcagc ggcgcggcgg tcgcgctcga
     3541 aatgtcggtc atccgtcgct gctagtcggg agaacggggc ggagtcaagc tttggcgcgc
     3601 taaggcccga ggaccgtcag ttccgtgaag gtcagatcgc cgaggcgcgg ctgcgccggc
     3661 cctttgaaat atttcggccc gatatcgggc aagtaccggc cgatcaatcc ggggagcggc
     3721 ttgtttgcgt ccgtgatgca aacgacgccg ccgcgcgcca gcaaatagcg gccaagcgcg
     3781 ccggcatagg cgacgaaatc gccgaggtcg cgacaataga tgagctgttc gcaggggatc
     3841 gagccgcgca tcaccttgcg cttcacgaaa atgaagggat gcgcttcgcc ctccgccatg
     3901 acgatcagcg accggcaccc gagcgccgca tgtttgtcga ggatgtcgcg atcataagcc
     3961 gacaaaagcg ccgcttccgg ccgatcggac gaaaaactca acacgcg
//
LOCUS       DATGJW010000103         2862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_226448, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000103 DATGJW010000000
VERSION     DATGJW010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2862
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_226448"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VKU03_03015"
     CDS             complement(<1..345)
                     /locus_tag="VKU03_03015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891515.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLJ70260.1"
                     /translation="MRAPRKFFEPLAIGAPRPRDPPPRLERMIHFIPPHIEKLRAKAG
                     EIAAEVDVLLGNLEDAIPIDAKHAARAGFIAMAKATDFGSTALWARVNALNSPWILDD
                     MAQLVGEAGDKLD"
     gene            complement(422..2179)
                     /locus_tag="VKU03_03020"
     CDS             complement(422..2179)
                     /locus_tag="VKU03_03020"
                     /inference="COORDINATES: protein motif:HMM:NF024893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AsmA family protein"
                     /protein_id="HLJ70261.1"
                     /translation="MRPRLKWAALLFAAALAAAAGAYRWPLASAHVRAEFGRQLADAV
                     GLELRGPARAYLTLLPLPTIQLVDVELSGADGATLIVAPQVKARIALGPLLAGRLRLS
                     SAILRRPTILVDLDRSPFAKSSALARLIASKNPSAGETPFGAFRVERGLVHLVSAENG
                     LDTLVEDASGELDWPRLADPARLDLRARWRGAPVTIAARLDAPAQWLARQSSPAFLDI
                     TSPIGALKLAGALKSGEKNGFEGSVSADFASTAALARLFGARPTSFPPDGRLALAGKI
                     VFGRHILTLSDMRLEALAQSVDGALALTRNPSGWTLSGSLAAGALSLDEILANAPAIY
                     DESGWSDAPLSAGDFGALALDLRASIAKLQWRNHVLQDAALSLMSRDGRVTATLSEAT
                     AYKGLLKGEASFAPDERGVEMRATASLANADIGALLADFGATGYAGQGGGQFSVHAAG
                     DSPAALARALTGDASVALGPGAIEGLSFEEALRRSERRPINLFADMRTGRTVFNEAAA
                     KATIEKGEAHLRSAAMLGPGLLITMKGGADLAARQLAAQITAARADEHGAPNPGGPEI
                     HVTITGPWARPLIKSDTGA"
     gene            complement(2299..>2862)
                     /locus_tag="VKU03_03025"
     CDS             complement(2299..>2862)
                     /locus_tag="VKU03_03025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLJ70262.1"
                     /translation="PNDVVVKSDGSIWFTDPTYGIDSQYEGDAAPSEIGASNVYRIDP
                     QSGALSAVATDFVKPNGIAFSPDESELFVVDTGATHVKNGPRHIRAFAVNPDGKSLGQ
                     GRVFATCTVGLFDGFRFDTKGHVWTSAGDGVHCYAPDGALIGKIKVPEVVANVCFGGP
                     KRNRLFICGTTSLYAIYVNAQGATWPR"
BASE COUNT          466 a          947 c         1004 g          445 t
ORIGIN      
        1 gtcgagcttg tcgccggcct cgccgacgag ctgcgccatg tcgtcgagaa tccatggcga
       61 gttcagcgcg ttgacgcgcg cccacaaggc cgtcgagccg aaatcggtcg ccttggccat
      121 ggcgatgaag ccggcgcgcg ccgcgtgctt ggcgtcaatc ggaatggcgt cttcgagatt
      181 gccgagcagg acgtcgactt ccgcggcgat ctcgccggct ttggcgcgca gcttttcgat
      241 atgcggcggg atgaaatgaa tcatccgctc gagccgcggc ggcggatcgc gcgggcgcgg
      301 cgcgccgatg gcgagcggct cgaagaattt gcgaggggcc ctcatggcgc gctcccggcg
      361 tcttgcgccg caatagaact gatcgcgtgg cgtcgcgcaa tgctgccgcg ccgccgtcga
      421 cttaagcgcc ggtgtccgac ttgatcagcg gccgcgccca ggggccggtg atcgtcacgt
      481 ggatttccgg gccgcccgga ttgggcgcgc cgtgctcgtc ggcgcgcgcc gcggtgattt
      541 gcgcggccaa ttggcgcgcg gcgaggtcgg cgccgccttt catcgtgatc aggaggccgg
      601 gcccgagcat cgcggcgctg cggagatgcg cctcgccctt ctcgatcgtc gccttggcgg
      661 cggcttcatt gaacacggtc cgcccggtcc gcatatcggc gaagagattg atcggccgcc
      721 gctcgctgcg cctcaacgct tcttcgaagc tcaagccttc gatcgcgccg gggccgagcg
      781 cgaccgaagc gtcgcccgtc aacgctctcg ccaaagccgc cgggctgtcg ccggccgcgt
      841 gtaccgaaaa ttggccgccg ccctggccgg catagccggt cgcgccgaaa tcggcgagca
      901 atgccccgat atcggcgttg gcgaggctgg ccgtcgcgcg catctcgacg ccgcgctcgt
      961 ccggcgcgaa ggacgcctcg cctttgagca ggcctttata ggcggtcgcc tcagacaggg
     1021 tcgccgtcac ccggccgtcg cgcgacatca gcgacaaagc cgcgtcctgc agcacatgat
     1081 tgcgccattg cagcttggcg atcgaggcgc gcaaatcgag ggccagggcg ccgaaatcgc
     1141 cagccgatag cggcgcatcg ctccagccgc tctcgtcgta gatcgccggc gcattggcga
     1201 ggatttcgtc gagcgacagc gcgcccgccg cgagcgagcc cgacagcgtc cagccggacg
     1261 gattgcgcgt caacgccagc gcgccgtcga cgctctgcgc cagcgcttcg aggcgcatat
     1321 cgctcaaagt caggatatgc cggccaaaga cgatcttgcc ggccaaagcg agccgcccgt
     1381 ccggcggaaa gctggtcggg cgagcgccga acagccgcgc cagcgccgcg gtcgatgcga
     1441 aatcggcgct gacggagcct tcgaacccgt tcttttcgcc cgatttgagc gcgcccgcca
     1501 atttcagcgc gccgattggc gaggttatat cgagaaaggc cggcgagctt tgtcgggcga
     1561 gccattgcgc cggcgcgtcg aggcgcgccg cgatcgtcac cggcgcgccg cgccagcggg
     1621 cgcgcagatc gagccttgcc ggatcggcga ggcgtggcca gtcgagctcg ccggacgcgt
     1681 cttcaaccag cgtgtcgagt ccattctcgg cgctgacgag atggacgagc ccgcgctcga
     1741 cgcggaacgc gccgaaaggc gtctcgccag ccgagggatt tttcgacgcg atgaggcgcg
     1801 cgagcgcgct gctcttggcg aaaggcgacc ggtcgagatc gacgaggatg gtcggccgcc
     1861 gcaaaatcgc gctggaaagc cgcagtcggc cggcgaggag cgggcccaaa gcgatgcggg
     1921 ctttgacctg gggcgcgacg atgagcgtgg cgccatcggc gccgctgagt tcgacatcga
     1981 cgagctgaat ggtcggcagc ggcaggagcg tcagataggc ccgggccggg ccgcgcaatt
     2041 cgaggccgac agcgtcggcc aattgccggc cgaattccgc ccggacatgg gcggaggcga
     2101 gcggccatct ataggcgccg gccgcggcgg ccagcgccgc cgcgaacaat aaggccgccc
     2161 atttcaggcg aggtcgcatc caaccttcca tgttctcgga cgaatcggcc gcctcgtccg
     2221 agaggccaga gtcccgccta tagaccaatt cgcggcgcga agcccgccta aattgtcggc
     2281 cgtcaaccgg cggcggcgtc aacgcggcca ggttgcgccc tgggcgttga catagatcgc
     2341 atagagcgag gtcgtgccgc aaatgaacag gcggttgcgc ttggggccgc cgaaacagac
     2401 attggcgacc acttccggaa ccttgatctt gccgatcagc gcgccgtccg gggcatagca
     2461 atggacgccg tcgccggccg aggtccagac atggcccttc gtgtcgaagc ggaacccgtc
     2521 aaacaagccg acggtgcagg tcgcgaaaac ccggccctgg ccgagcgatt tgccgtcggg
     2581 gttgacggcg aaggcgcgaa tatggcgcgg gccgttcttg acatgcgtcg cgccggtgtc
     2641 gacgacgaag agctcgctct cgtccggtga aaaggcgatc ccgttcggct tgacgaaatc
     2701 ggtcgcgacc gcgctcaaag cgccgctttg cggatcgatg cggtagacgt tgctggcgcc
     2761 gatctccgag ggcgcggcgt cgccttcgta ctgcgagtcg atgccgtagg tcggatcggt
     2821 gaaccagatc gagccgtcgg acttgacgac gacgtcgttg gg
//
LOCUS       DATGJW010000104         7221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_952195, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000104 DATGJW010000000
VERSION     DATGJW010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7221
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_952195"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..742
                     /locus_tag="VKU03_03030"
     CDS             <1..742
                     /locus_tag="VKU03_03030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HLJ70263.1"
                     /translation="VIAVLPVAAIEQHGPHLPVGVDTFINEGYLARTIARVPPELPVL
                     FLPVQTIGKSNEHIEFPGTLTFTAETLIRAWFEIGESVARTGCRKLIFVNSHGGNVSI
                     IDVVARELRVRRNMLVVNSAWSRFGYPDGLFGPKERAHGIHAGDAETSLMLAFRPNTV
                     KMAEARDFVSAAVGMEQDYTWLRATQPIGMGWMSQDLNDFGAMGDASAATLQKGEACA
                     DYGATAFIELLADVDAFDLARLGKGPLG"
     gene            739..1401
                     /locus_tag="VKU03_03035"
     CDS             739..1401
                     /locus_tag="VKU03_03035"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003533269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HLJ70264.1"
                     /translation="MKRERKPAPAEPPRPLPDFVQEQAHIAEGLWPVAGVDEAGRGPL
                     AGPVAVAAVILNPADLPEGVDDSKALTAEQREPLFDLILAKALGVSLAFASADEIDAH
                     NIRMATLRAMARAVGALHASPRIVLIDGRDLPPGLSCRSRAIVAGDATCLSIAAASIV
                     AKVARDRMMARLDSHCPGYGFARHAGYATRLHLDAIAALGPSPFHRRSFSPVKLDDIR
                     AE"
     gene            1674..2852
                     /locus_tag="VKU03_03040"
     CDS             1674..2852
                     /locus_tag="VKU03_03040"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009008 - DNA-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HLJ70265.1"
                     /translation="MRLSHAGATGQPAQLQVLRTGRGSARRPGEVPPPRDQILVGDCV
                     ATLDSLPAECADLVFADPPYNLQLEGALSRPDQSLVDAVDDDWDKFASFAEYDSFTRR
                     WLAAARRVMKRDATIFVIGSYHNIFRVGAIMQDLGFWILNDIVWRKANPMPNFRGRRF
                     TNAHETLIWAARSADAKKYTFNYETLKAGNEDCQARSDWLLPICSGGERLKDSSGRKT
                     HPTQKPEALLARVLLAASNPGDLVLDPFFGSGTTGAVAKKLRRSYLGVEREARYARAA
                     EARIAAIEPLAQETVLAAPTRRSEPRVAFASLVESGLVRAGEKLTDAQRRHRALIRPD
                     GAISLGPIVGSIHKIGALAQGLPACNGWTFWHVERGGALTPIDDMRASVRATMRAAAE
                     "
     gene            complement(2958..3896)
                     /gene="prmB"
                     /locus_tag="VKU03_03045"
     CDS             complement(2958..3896)
                     /gene="prmB"
                     /locus_tag="VKU03_03045"
                     /EC_number="2.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008276 - protein methyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA];
                     GO_process: GO:0042273 - ribosomal large subunit
                     biogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3 N(5)-glutamine
                     methyltransferase"
                     /protein_id="HLJ70266.1"
                     /translation="MAAAPADETEGFVTLRDVFRYAVSRFNAAKLAYGHGTTNAVDEA
                     AFLLLEALRLPIDRLDPFLDARLTPSERGKLLALIEARIATRKPAAYLLNRAYIQGRP
                     FYVDERAIVPRSFIGELLFGGLVGAEGLIQAEEVVSVLDLCTGGGSLAILAAEIFPAA
                     SIDAVDCSADALAVARRNVEEYGLADRVALLQGDLFAPVGDKRYDLILSNPPYVDDEA
                     LNAFPPEFAAEPRLAHQGGRDGLDIVRRIVAEAGAHLSKRGALVCEIGRGRAAFEQDF
                     PGLSPIWLDTAESEGEVFLLRAPILPRRRRGRETAR"
     gene            complement(3979..4206)
                     /locus_tag="VKU03_03050"
     CDS             complement(3979..4206)
                     /locus_tag="VKU03_03050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006018777.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PsiF family protein"
                     /protein_id="HLJ70267.1"
                     /translation="MKLSAIVSAGVAVMFSASLAFAQASTPAPAAPANAAAKAARSKE
                     CSKEADAKGLHGKARKEFRAKCKRGEDAGKM"
     gene            4391..5098
                     /locus_tag="VKU03_03055"
     CDS             4391..5098
                     /locus_tag="VKU03_03055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase beta subunit-related
                     protein"
                     /protein_id="HLJ70268.1"
                     /translation="MTTISIAALRERFPDFRLALIVARDLTVKPHRSAALDRAIGEAE
                     RACRAQWSGVELSAIPAVAAWRTAYRGFGVKKTSYRSSVERLIKRVLAGGELPRVNNL
                     VDLYNCVSLTHGLCLGADDLDKTSGDLAFRFAQPGDTFIDMSAEPGEDPNDPPKPGEV
                     VYADSRHVLCRRWNWRQDARTALSAETRRAVLTAQSNGFGEVEAAAEALARGVAAECG
                     GQASVVVLDRTKPVGEF"
     gene            5141..6169
                     /locus_tag="VKU03_03060"
     CDS             5141..6169
                     /locus_tag="VKU03_03060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLJ70269.1"
                     /translation="MSKETLALDLPKARLQDLLAGLAAAGEITRLRLLCLLAEAELTV
                     TELVAILGQSQPRVSRHLKLLTEAGLAERRREGAWAFFRLGETGAGVALARDIVERID
                     PADPIVAGDRARLAMTREARRRQAAAYFARMAEDWDRIRALHAPEERVEAALAAMLGG
                     KPVRALLDLGTGTGRMIELFAPLAQRAVGVDQSPAMLALARSRIDQTGLRNVQLRQGD
                     IYAPPVERNAFDLVVIHQVLHFLDDPARALVEAARALRPGGRLVVVDFAAHDEEYLRE
                     TFAHRRLGFTAGEIESFLAEAGLDIDETRAIPPAAGEAGKLTVTIWLAKDPRILADPP
                     PQHIREIA"
     gene            6173..7129
                     /gene="metF"
                     /locus_tag="VKU03_03065"
     CDS             6173..7129
                     /gene="metF"
                     /locus_tag="VKU03_03065"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase [NAD(P)H]"
                     /protein_id="HLJ70270.1"
                     /translation="MQSPLRPSRSGPEVRVSFEFFPPKTAEMEQTLWEAIARLAPLAP
                     HFVSVTYGAGGSTRERTHATVARIVEETALKPAAHLTCVGASRAEIDGVIRDYSAAGV
                     RHVVALRGDPPDGAGSSYQPHPDGYQSTDALVKSLKTLGDFQVSVATYPEKHPQSPTL
                     LHDIEILKRKVDSGADRAITQFFFDNSSYFRFLDVAAAAGIDIPIVPGIVPVQNFKQT
                     AGFAKRAGASVPAWLADRFDGLDDDPVTRRLVAAAVCAEQVVDLIDRGVRDLHFYTMN
                     RADLVFAICHLIGLRPQKAAPEAAWTLAAAGAAASAAQPTTR"
BASE COUNT         1156 a         2520 c         2338 g         1207 t
ORIGIN      
        1 cgtcatcgcc gtcctgccgg tcgcggcgat cgagcagcac gggccgcatc tgccggtcgg
       61 cgtcgacacc ttcatcaatg aaggctatct cgcgcgcacg atcgcgcgcg tgcccccgga
      121 attgccggtc ctgttcctcc ccgtccagac gatcggcaag tcgaacgagc atatcgaatt
      181 tcccggcacg ctgaccttca ccgccgaaac gctgatccgc gcctggttcg agatcggcga
      241 gagcgtcgcc cggaccggct gccgcaagct gatcttcgtc aattcgcatg gcggcaacgt
      301 gtcgatcatc gacgtcgtgg cgcgggaatt gcgcgtccgc cgcaacatgc tggtcgtcaa
      361 ctcggcgtgg agccggtttg gctatcccga cggcctgttc ggcccgaaag agcgcgccca
      421 cggcattcat gccggcgatg ccgagacctc gctgatgctc gccttccggc cgaacacggt
      481 gaagatggcg gaggcgcgcg atttcgtcag cgcggccgtg ggaatggagc aggattacac
      541 ctggctgcgc gcgacgcagc cgatcggcat gggctggatg tcgcaggacc tcaacgattt
      601 cggcgcgatg ggcgacgcct cggcggcgac gctgcaaaag ggcgaagcct gcgccgacta
      661 tggcgcgacc gcctttatcg aattgctcgc cgatgtcgac gcattcgatc ttgcgcggct
      721 cggcaagggg ccgctcgggt gaagcgcgag cgcaagcccg ctcctgccga gccgccgcgc
      781 ccattgccgg atttcgtcca agaacaggcc catatcgccg aagggctctg gccggtcgcc
      841 ggcgtcgatg aagccggccg cgggccgctt gccgggccgg tcgcggtcgc cgccgttatc
      901 ctcaatcccg ccgatctgcc ggaaggcgtc gacgattcga aagcgctgac cgccgagcag
      961 cgcgagccat tgttcgatct catcctcgcc aaggcgcttg gcgtgtcgct tgctttcgcc
     1021 agcgccgacg agatcgatgc gcacaatatc cgcatggcga cgttgcgcgc catggcgcgc
     1081 gccgtcggcg cgctgcatgc ttcgccgcgc atcgtcttga tcgatgggcg cgatctgccg
     1141 cccggccttt cctgccgctc gcgcgcgatc gtcgccggcg acgcgacatg cctgtcgatc
     1201 gccgctgctt cgatcgtcgc caaagtggcg cgcgaccgga tgatggcgcg gcttgattcg
     1261 cattgtccgg gctatggctt cgcccgccac gccggctatg cgacgcggct gcatctcgac
     1321 gcgatcgccg cgctcggccc ctcgcccttc catcggcgct cgttcagccc ggtcaagctc
     1381 gacgacattc gcgccgaata gacgtcacgc ccgacgcatt tgcagccgcg gtcgccgact
     1441 gcggctgagc gaggggcgcg gtcaacgacc gcgtcgacaa gccgcgctga gcgccggaga
     1501 gatcagcgat cgcctggaac ggcaaaaagt cttaccaaac aattgaaatc gccgtctttt
     1561 atttcttccc gccgcaccaa aacgaaacag ccgcgaagcc gcgccgaaaa ctcaaatttt
     1621 actcaaatgc gccagattgc atccgcgtta gtcgcgtttg gcggttcgag ttcatgcgtc
     1681 tttcccacgc cggggcgacc ggccagcccg cccagcttca ggttttgcgt actgggaggg
     1741 ggtcggctcg ccgccccggc gaggttccgc cgccgcgcga ccaaatcctg gtcggcgatt
     1801 gcgtcgcgac gctcgattcc ctgccggccg aatgcgccga tctcgtcttc gccgatccgc
     1861 cctacaatct gcagctcgaa ggcgccttgt cgcgccccga ccagagcctg gtcgacgccg
     1921 tcgacgacga ttgggacaag ttcgccagct tcgccgaata cgattcgttc acgcggcgct
     1981 ggcttgccgc ggcgcggcgc gtgatgaagc gcgacgcgac gattttcgtc atcggctcct
     2041 accacaacat tttccgcgtc ggcgcgatca tgcaggatct cggcttctgg atcctcaacg
     2101 acatcgtctg gcgcaaagcc aatccgatgc cgaatttccg cggccgccgc ttcaccaacg
     2161 cgcatgagac gctgatctgg gcggcgcgca gcgccgacgc gaagaagtac accttcaatt
     2221 acgaaacctt gaaagccggc aacgaggatt gccaggcgcg ctcggactgg ctgctgccga
     2281 tctgctcggg cggcgagcgc ttgaaggatt cgtccgggcg caaaacccat ccgacccaga
     2341 agccggaggc gctgctcgcc cgcgtcctgc tcgccgcctc gaatcccggc gacctggtgc
     2401 tcgatccttt cttcggctcg ggcacgaccg gcgccgtcgc caagaaattg cgccgctcct
     2461 atctcggcgt cgagcgcgaa gcgcgctatg cgcgggcggc cgaggcgcgc atcgcggcga
     2521 tcgagccgct ggcgcaggag acggtcctgg ccgcgccgac ccggcgcagc gagccgcgcg
     2581 tcgcctttgc gagcctcgtc gaatccggcc tggtgcgggc cggcgaaaag ctgaccgacg
     2641 cgcaacggcg ccatcgcgcg ctgatccggc ccgacggggc gatcagcctc gggccgatcg
     2701 tcggctcgat ccacaagatc ggcgcgcttg cgcaaggcct tcccgcctgc aacggctgga
     2761 cgttctggca tgtcgagcgc ggcggcgcgc tgacgccgat cgacgacatg cgcgccagcg
     2821 tccgcgcgac gatgcgcgcc gcggccgagt agctctcctc ccccgcgaaa gcgggggagg
     2881 gggaccgccc gaagggcggt ggtgggggcc gccgatgctt gaggataatc ttcggcgccg
     2941 tggccccctc caccgcgcta tcgcgcggtc tccctccccc gtcttcgacg ggggaggatg
     3001 ggcgcccgca gcaaaaacac ctcgccttcg ctctcggctg tgtcgagcca gatcggcgac
     3061 agaccgggaa aatcctgctc gaaagccgcc cggccgcggc cgatctcgca gaccagcgcg
     3121 ccgcgcttgc tgagatgcgc gccggcttcg gcgacgatgc gccgcacgat atcgagcccg
     3181 tcgcgcccgc cctgatgggc aagacgcggc tcggcggcga attccggcgg aaaggcgttc
     3241 agcgcctcat cgtcgacata gggcgggttg ctcaagatga gatcgtagcg tttgtcgccg
     3301 accggcgcga aaagatcgcc ttgaaggagc gcgacccgat cggcaaggcc gtactcttcg
     3361 acattgcgcc gcgccacggc gagggcgtcg gccgaacaat cgacggcgtc gatcgacgcg
     3421 gcgggaaaaa tctcggccgc gaggatcgcc agcgagccgc cgccggtgca aagatcgagc
     3481 acgctgacga cctcctccgc ttgaatcaag ccttccgcgc cgaccaggcc gccgaacagc
     3541 aattcgccga taaagctgcg tgggacgatc gcccgctcgt cgacatagaa aggccggccc
     3601 tggatataag cgcgattgag cagataggcg gcgggcttgc gcgtcgcgat gcgcgcctcg
     3661 atcaacgcga ggagcttgcc gcgctcgctc ggcgtcagcc gcgcgtcgag gaaaggatcg
     3721 agcctgtcga tcggcaggcg cagcgcttcg agaagcagaa acgccgcttc gtcaaccgcg
     3781 ttggtcgtac catgaccgta ggcgagcttg gccgcattga accggctcac cgcgtagcgg
     3841 aagacgtcgc gcagagtgac gaagccttcc gtttcgtccg ccggcgccgc ggccatgtcg
     3901 tttcaaccct ttccggacgt acagaaaatg gccgggcctc gcccggccat tcctctaaag
     3961 ctattcctca ggcgtggctc acatcttgcc ggcgtcttcg ccgcgcttgc attttgcgcg
     4021 gaactccttg cgcgccttgc cgtgcagccc cttggcgtcg gcctctttcg agcattcctt
     4081 cgatctggcc gccttggccg cggcgttggc cggcgccgcg ggagcgggag tcgaggcttg
     4141 cgcgaaagca aggctcgcgc tgaacatgac ggccacgccg gccgagacga ttgcggagag
     4201 cttcatgggc gtttctttct ggttggatcg ttgaagaccg cttttgcgga ccaactggcc
     4261 tcgtccgagc gatcgagcga tttatagtat cgcgcgacgg atcaatacaa gccgtctgcg
     4321 ccgaccaaag gcgcctctcg cgacgagagc ggccgcttgc gacgcgatat ttttaacgta
     4381 tggaaggccg atgacaacaa tctccatcgc ggcgcttcgc gagcgtttcc ccgacttccg
     4441 cctcgcgctc atcgtcgcgc gcgatttgac cgtcaagccg catcgctcgg ccgcgctcga
     4501 tcgagcgatc ggcgaagcgg aaagggcatg ccgcgcgcaa tggagcggcg tcgagctttc
     4561 ggccattccc gccgtcgcgg cgtggcgtac ggcctatcgc ggcttcggcg tcaagaagac
     4621 cagctaccgc tcctcggtcg agcgcctgat caagcgcgtt ctcgccggcg gcgagctgcc
     4681 gcgcgtcaac aatctcgtcg atctctacaa ttgcgtgtcg ttgacgcatg gcctctgtct
     4741 cggcgccgac gacctcgaca agacgtccgg cgatctggcg tttcgattcg cgcagcccgg
     4801 cgacacgttc atcgacatga gcgccgagcc gggcgaagac ccgaacgatc cgcccaaacc
     4861 cggcgaggtc gtctacgccg attcgcgcca cgtcctgtgc cggcgctgga actggcgcca
     4921 ggacgccagg accgccttat cggcggagac ccggcgcgcc gttctcaccg ctcaatcgaa
     4981 tggattcggc gaggtcgagg ccgccgccga ggcgctggcc cgcggcgtcg cggccgaatg
     5041 cggcggacag gccagcgtgg tcgttctcga tcggacgaag cctgtcggag aattttgatt
     5101 tcgcttgctc attcgcgaaa ttggacataa atatatcttt atgtctaaag agacgctcgc
     5161 gctcgacctg ccaaaggccc gcctgcaaga tctcctggcc ggcttggcgg cggcgggcga
     5221 gatcacgcgg cttcgccttc tctgcctcct ggccgaggcc gagctgaccg tcaccgagct
     5281 cgtcgccatt ctcggccagt cgcagcctcg cgtctcccgc catctcaagc tgctgaccga
     5341 ggccggcctc gccgagcgcc ggcgcgaagg ggcctgggcc tttttccgct tgggcgaaac
     5401 cggcgccggc gtcgccctgg cccgcgacat cgtcgaaagg atcgatcccg ccgacccgat
     5461 cgtcgccggc gaccgcgcgc ggctcgccat gacgcgcgag gcgcgccgcc gccaggccgc
     5521 cgcctatttc gcccggatgg ccgaggactg ggaccgcatc cgcgccctgc acgcgccgga
     5581 agagcgggtc gaagcggctt tggccgcgat gctcggcggc aagccggtcc gcgcgctgct
     5641 cgatctcggc acaggaacgg gccgcatgat cgaactcttc gcgcccctgg cgcagcgcgc
     5701 cgtcggcgtc gatcagagcc ccgccatgtt ggccctcgcc cgctcgcgca tcgaccagac
     5761 cggcttgcgc aatgtgcaat tgcgacaggg cgatatctat gccccgccgg tcgagcgcaa
     5821 cgctttcgat ctcgtcgtca ttcaccaggt gctgcacttc ctcgacgatc ccgcccgcgc
     5881 tttggtcgag gcggcgcgcg cgctgcggcc cggcggccgc ctcgtcgtcg tcgatttcgc
     5941 cgcgcatgac gaagaatatc tgcgcgagac gttcgcccat cgccggctcg gcttcaccgc
     6001 cggagaaatc gaatccttcc tcgccgaggc ggggctcgac atcgatgaga cgcgcgccat
     6061 cccgcctgcc gcgggcgaag ccggcaagct caccgtcacg atttggctcg ccaaggaccc
     6121 gcgcattctc gccgacccgc cgccgcaaca catccgggag atcgcgtaag tgatgcagag
     6181 cccgcttcgt ccgagccgct caggcccaga ggttcgcgtc tccttcgagt tctttccgcc
     6241 caagacggcg gagatggagc agacgctgtg ggaggcgatc gcgcggctcg cgccgctcgc
     6301 gccgcatttc gtctcggtca cctacggcgc cggcggttcg acgcgcgaac gcacccacgc
     6361 gaccgtggcg cgcatcgtcg aggagacggc gctgaagccg gcggcgcatt tgacctgcgt
     6421 cggcgcgagc cgggccgaga tcgacggcgt gatccgcgat tatagcgccg cgggcgtgcg
     6481 ccatgtcgtc gccttgcgcg gcgatccgcc cgacggcgcc ggctcgtcct accagcccca
     6541 ccctgacggc taccaatcga cggacgcgct ggtgaagagc ttgaagacgc tcggcgattt
     6601 tcaagtgtcg gtcgccacct accccgagaa gcatccgcag agcccgacgc tgctgcacga
     6661 catcgagatt ctcaagcgca aggtcgattc cggcgccgac cgggcgatca cgcaattctt
     6721 tttcgacaat agcagctatt ttcgctttct cgatgtcgcg gcggcggcgg ggatcgacat
     6781 tccgatcgtg ccgggaatcg tgccggtgca gaatttcaag cagaccgccg gcttcgccaa
     6841 gcgcgccggc gcgagcgtgc cggcctggct cgccgatcgt ttcgacgggc tcgacgacga
     6901 tcccgtgacc cgccgcctcg tcgccgccgc ggtctgcgcc gagcaggtcg tcgacctgat
     6961 cgaccgcggc gtgcgcgact tgcatttcta tacgatgaac cgcgccgacc ttgtcttcgc
     7021 gatctgccat ctgatcgggc tgaggccgca aaaggcggcg ccggaagcgg catggacgct
     7081 ggctgccgcc ggcgcggcgg cgagcgcggc gcaaccgacc acgcggtagc gtcttccctc
     7141 gccccgcgta acgcgcaaag cgggagaagg ggacatactc ctcaccgcgg cgcgccgggc
     7201 gcgtcgagga aacgctgcat g
//
LOCUS       DATGJW010000105         4050 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_123473, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000105 DATGJW010000000
VERSION     DATGJW010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4050)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4050)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4050
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123473"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..617)
                     /locus_tag="VKU03_03070"
     CDS             complement(<1..617)
                     /locus_tag="VKU03_03070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70271.1"
                     /translation="MLFDILSAIDITGAAAIVVAFLALALPSAAARLRAALVLAAWLL
                     VVIALGATDALAAQYAGPPGLALAVVVPVAALCWVFFASSAGRAALLATPLWAPIALN
                     ALRLLGVSFVVLYAAGRLPAPFAPSAGWGDIFIGATAVPLAFLVARYGARANGLVLAW
                     NVIGVLDLVFAIGFGATSSPGPIQIFVGSPTSAMMTTLPWLIVPA"
     gene            784..1242
                     /locus_tag="VKU03_03075"
     CDS             784..1242
                     /locus_tag="VKU03_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HLJ70272.1"
                     /translation="MDIRIEEVLGCTCLRMRRATRRITQIYDHALAPAGVTVNQFGLM
                     AHLYGASLAGRPGFAIHVLAVRLGADPTTLNRTLRPLQENGLIRNVGDPSDRRVRLVQ
                     LTDKGRRELAKVMPLWRAAQAQVEKSLGQKSLRELNELLDFAAAKLEPGQ"
     gene            1337..1642
                     /gene="clpS"
                     /locus_tag="VKU03_03080"
     CDS             1337..1642
                     /gene="clpS"
                     /locus_tag="VKU03_03080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497217.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HLJ70273.1"
                     /translation="MSDIATKPRTKTKPKTERPKLHKVILLNDDYTPREFVVTILKAE
                     FRMNEARAYAVMMTAHRKGACVVAVFAKDVAETKATRATEAGKAKGYPLMFTTEPEE"
     gene            complement(1751..2188)
                     /locus_tag="VKU03_03085"
     CDS             complement(1751..2188)
                     /locus_tag="VKU03_03085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ70274.1"
                     /translation="MSDEKPKNEEPYILDAQVGFILRQAQQRHTTLFASRMIEGLTPT
                     QWAALAKLSEIGPSSQNQLGRLTAMDAATIKGVIDRLSKRGFTATKPDPADGRRLLVA
                     LTADGEALYGKAAPIAKRITEETLAPLSERERAQFIALLSRLT"
     gene            complement(2290..3006)
                     /locus_tag="VKU03_03090"
     CDS             complement(2290..3006)
                     /locus_tag="VKU03_03090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70275.1"
                     /translation="MSARAKLSVGNLNAAYGRAHILFGVSLDVAAGEAVALIGRNGAG
                     KTTTFRAILGLIANRSGAIRFDGEDVSRLAAYEIARRGLGYVPEDRRIFADLTVSENL
                     EVGRQKPRPGAPQWTTERLFEIFPNLAETRGRLGGRLSGGEQQMLTIARTLMGNPSLV
                     LLDEPSEGLAPKIVEQMIAAILEMKREGLSLLISEQNLHFARLVADRVAVIEKGRIRF
                     AGAMAEFDARPDIRDAYLAL"
     gene            complement(3003..3764)
                     /locus_tag="VKU03_03095"
     CDS             complement(3003..3764)
                     /locus_tag="VKU03_03095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009025535.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70276.1"
                     /translation="MILLEVDNLGKEYSGFEAVKGVSFSLRAGEMLALIGPNGAGKST
                     CFNMINGQIAPSTGRIAIFGRSAAGLKPRDIWRLGVGRTFQVAAAFASMSVAENVQVA
                     LLSHHRRQFRLFGWAGRFYAEEAAALLALVGMKEQAGRSCGELAYGDLKRLEFALALA
                     NDPKLLLMDEPTAGMAPKERGALMALVAQIARERKIGVLFTEHDMDAVFAYADRILVL
                     NRGVLIAEGSPVSVRADPQVQAVYLGAGSLYGAAH"
     gene            complement(3761..>4050)
                     /locus_tag="VKU03_03100"
     CDS             complement(3761..>4050)
                     /locus_tag="VKU03_03100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70277.1"
                     /translation="FAFLKGSVFPDTLGIPVSIDGLVMVLLGGVGTVSGGVIGALIYK
                     TLSIFVVSQTDYSKLVLGLLIVALVALFPQGLLGAWQAWRSGGFKPLSKPS"
BASE COUNT          778 a         1290 c         1361 g          621 t
ORIGIN      
        1 aaggccggaa cgatgagcca gggcagggtc gtcatcatcg cgctggtcgg cgagcccacg
       61 aaaatctgaa tcgggccggg cgaggaggtc gcgccgaagc cgatggcgaa gacgagatcg
      121 aggacgccga tgacgttcca ggcgaggacg aggccgttgg cgcgggcgcc gtagcgcgcg
      181 acaagaaacg ccagcggaac ggccgtcgcg ccgatgaaaa tatcgcccca gcccgccgaa
      241 ggcgcgaagg gcgccggcag ccggccggcg gcgtagagca ccacgaagga gacgccgagg
      301 aggcgaagcg cgttgagtgc gatcggcgcc caaaggggcg tcgccagcag cgcggcgcgt
      361 ccggcggacg aagcgaaaaa gacccagcac agcgcggcga ccggaacgac gaccgcgagg
      421 gcgaggccgg gcggcccggc atattgggcc gccagcgcat cggtcgcgcc gagcgcgatg
      481 acgaccagga gccaagccgc cagaaccagc gcagcgcgca gcctcgcggc cgcgctcggc
      541 agggcgagag cgagaaaagc aacgacgatt gcggcggcgc cggttatgtc gatggccgag
      601 aggatatcga agagcatggg tccctcttct cgatgcggaa ttaagatgta tatacaggta
      661 tttgagccgg cgccgggctg aggcgttttg gcagccggct cagaacctgc gtatgcaggt
      721 atatcgcttc ggcgacgaag tcaaatatcg tcaggtctat ggcggtcgga gaggagcgga
      781 cgaatggaca ttaggatcga ggaagtcctc ggctgcactt gccttcggat gcgccgggcg
      841 acacggcgca tcacgcagat ttacgaccac gcgctggcgc cggccggggt cacggtcaat
      901 caattcggcc tcatggcgca tctttacggc gcgagcctcg ccgggcggcc gggattcgcc
      961 atccatgttc tcgccgttcg gctgggcgcc gatccgacca ccctgaaccg gacgctgagg
     1021 cctctccagg aaaatggcct gatccggaat gtcggcgatc cgtcggaccg gcgggtgcgg
     1081 cttgtccagc tcaccgataa ggggcggcgc gaactcgcca aggtgatgcc gctctggcgc
     1141 gcggcgcaag cccaggtgga gaagagtctt ggacaaaaat ccctgagaga gctgaacgaa
     1201 ttattggact ttgctgcggc gaagcttgag cccggtcaat aatccggcca gccggcgaag
     1261 ccgcgccgcc gcatggacgg gcggccttga atgtgagatt ctctttcgcc gcttagctcc
     1321 tgcttgcgcc gaaccgatga gcgacatcgc aaccaagccg cggaccaaga ccaagccgaa
     1381 gaccgagcgg ccgaagctgc acaaggtcat cctgctcaac gacgattaca cgccgcgcga
     1441 attcgtcgtc acgattctca aggcggaatt ccgcatgaac gaggcccgcg cctacgcggt
     1501 gatgatgacc gcgcatcgca agggcgcctg cgtcgtcgcg gtcttcgcca aagacgtcgc
     1561 cgagacaaaa gcgacccggg cgaccgaggc gggcaaggcc aagggttatc cgctgatgtt
     1621 cacgacggag ccggaggagt agggcgcttc cgttattgcg agaaccccga gcgcagcgag
     1681 gggacgaagc aatccattcg gagagcgacg tcctgcgaat ggatgcttcg cttcgctcgc
     1741 aacgacggat ttatgtcagc cgcgagagca gggcgatgaa ctgcgcccgc tcgcgctcgc
     1801 tgagcggcgc cagcgtctcc tcggtgatcc gcttggcgat cggcgccgcc ttgccgtaaa
     1861 gggcctcgcc gtccgccgtg agcgccacca aaagacgtcg cccgtcggcc gggtcaggtt
     1921 tggtcgccgt gaagccgcgt ttgctcagcc ggtcgatcac gcccttgatc gttgcggcgt
     1981 ccatcgcggt gagccggccg agctggttct gcgaggacgg gccgatctcg ctcagcttgg
     2041 cgagcgccgc ccattgcgtc ggcgtcaggc cttcgatcat gcgcgaggcg aagagcgtgg
     2101 tatggcgctg ctgcgcctgg cgcaggatga agccgacctg cgcgtcgagg atatacggct
     2161 cttcgttctt tggcttctcg tcgctcatca gaacctcaat gtcattcccg cacgagcgga
     2221 atccaggacc gccgttgccg caagctcggc gactgctgga ttcccgcttt cgcgggaatg
     2281 acacaggcct cacagcgcaa ggtaggcgtc gcgaatgtcg gggcgcgcgt cgaactcggc
     2341 catcgcgccg gcgaagcgga tccgtccctt ctcgatcacg gcgacgcggt cagcgaccag
     2401 cctcgcgaaa tgcaaattct gctcgctgat caaaaggctc aggccttcgc gcttcatttc
     2461 cagaatcgcg gcgatcatct gctcgacgat tttcggcgcg aggccctcgg aaggctcgtc
     2521 gagcaaaacc agcgacggat tgcccatcaa ggtccgcgcg atcgtcagca tctgctgctc
     2581 gccgcccgac aggcggccgc cgagccggcc gcgcgtctcg gcgaggttcg ggaaaatctc
     2641 gaacaggcgc tcggtcgtcc attgcggcgc gcccggccgc ggcttctggc ggccgacttc
     2701 gagattttcc gagaccgtca gatcggcgaa aatgcgccgg tcctccggca catagccgag
     2761 gccgcggcgg gcgatttcgt acgcggcgag ccgcgagacg tcctcgccgt cgaaacgaat
     2821 cgcgccgctg cgattggcga tcagtccgag aatggcgcga aaggtcgtcg ttttgccggc
     2881 gccgttgcgc ccgatcagcg cgacggcttc gccggcggcg acgtcgagcg agacgccgaa
     2941 aaggatatgc gcgcggccat aggcggcgtt gaggttgccg acgctgagct tggcgcgcgc
     3001 gctcaatgcg ccgctccgta caggctgccg gcgccgagat agaccgcctg cacctgcgga
     3061 tcggcgcgca ccgaaacagg cgacccctcg gcgatcagga cgccgcgatt gagcacgagg
     3121 atgcggtcgg cataggcgaa gaccgcgtcc atgtcgtgct cggtgaagag aacgccgatt
     3181 ttcctttcgc gcgcgatttg ggcgaccagc gccatcaggg cgccgcgctc cttcggcgcc
     3241 atgccggcgg tcggctcgtc catcagcagc aatttcggat cattggcgag agccagcgcg
     3301 aattccaggc gcttgagatc gccataagcg agctcgccgc aactccgccc ggcctgctcc
     3361 ttcatcccga ccagcgcaag cagcgccgcc gcctcttcgg cgtagaagcg cccggcccag
     3421 ccgaaaagac gaaactggcg gcgatgatgc gacagcagcg cgacctgcac gttttcggcg
     3481 accgacatcg aagcgaaagc ggcggcgacc tggaaggtcc ggccaacgcc aaggcgccag
     3541 atgtcgcgcg gcttcagccc ggcggcgctg cggccgaaaa tcgcgatccg gcccgtgctc
     3601 ggcgcgatct ggccgttgat catgttgaag caggtgcttt ttcccgcgcc gttcgggccg
     3661 atcagcgcca gcatctcgcc ggcccgaagc gagaagctca cacccttgac cgcttcgaag
     3721 ccggaatatt ctttgccgag attgtcgact tcaagaagga tcatgacggc ttgctcagcg
     3781 gcttgaagcc gccgctccgc caagcctgcc acgcgccaag cagcccttgc ggaaacaagg
     3841 cgacgagggc gacgatcagc agtccgagaa ccagcttgga ataatccgtc tggctgacga
     3901 cgaagatcga cagcgtttta taaatcagcg cgccgatgac gccgccggaa accgttccga
     3961 cgccgccgag caggaccatg acgaggccgt cgatcgacac ggggataccg agcgtgtcgg
     4021 gaaagacgct gcctttgaga aaggcgaaaa
//
LOCUS       DATGJW010000106         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1367108, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000106 DATGJW010000000
VERSION     DATGJW010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367108"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(313..1407)
                     /locus_tag="VKU03_03105"
     CDS             complement(313..1407)
                     /locus_tag="VKU03_03105"
                     /inference="COORDINATES: protein motif:HMM:NF041079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBASS cGAMP-activated phospholipase"
                     /protein_id="HLJ70278.1"
                     /translation="MTFRILALSGGGYLGLYSARILTRLEAAAGKPIARCFDLISGAS
                     AGAIAALALSLEIPAAEIEQVFIDHGPRIFPQINRPFREAKRTLAFLRSLLRPKYSDA
                     ALRAAIASLIDEDKTLASARHRLALPVVNMTTGQIEVIKTPHLANWTHHSGFKMVEVA
                     LAAAAAPTYFPMAELSNSLYVDGAIFANAPDLVGVHEAEYYLKTPVEEIEVLSIGTTT
                     GNFSLPYSHGRSYGSARWLRGGRLFATIMSAQQQLTQVFLRHHLGDRYLRVDTQRAPG
                     LEYDLTFDGATPHGTKTILSLADHAFEEAMKLPRVLAMTKAAAPDPTFYPGLPGSSAL
                     LPPPGEAATFDAAELRGPARIAAPVGRAAD"
     gene            complement(1523..2419)
                     /locus_tag="VKU03_03110"
     CDS             complement(1523..2419)
                     /locus_tag="VKU03_03110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010974313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HLJ70279.1"
                     /translation="MPPRANWKGVIRIGDLTCPVALYTAASTSERIAFHMLNRKTGDR
                     VHRQFVDQGAGKPVESDDQVKGYEASKGEYVIIEPEEIASAFPDSDKTLAVEHFIACD
                     EIDNLYFDRPYYLGPAEPVSQHAFALIREGMRKAKVAALARAVLFRRVRTVLMRPHEE
                     GMIATLLNFDYEVRSAEEAFAGIPRIKIQSEMLDLAKHIIKTKPGRFDPKAYDDRYEA
                     ALAELVEAKLEGRPIEAAKPARSTNVVDLMDALRRSAAAAGAPAAKASAKKPAAGRAG
                     GGKRKRAGKIGAAKRRAARKAS"
     gene            2566..2781
                     /locus_tag="VKU03_03115"
     CDS             2566..2781
                     /locus_tag="VKU03_03115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007602912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70280.1"
                     /translation="MARISYTVAPTADEWSVFRDGERGMSYATQEAAFEVAIAEASGD
                     LRSGHEVVIEVRAAFADPVDRRPSERL"
     gene            complement(2802..>3178)
                     /locus_tag="VKU03_03120"
     CDS             complement(2802..>3178)
                     /locus_tag="VKU03_03120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014857865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF3775 domain-containing protein"
                     /protein_id="HLJ70281.1"
                     /translation="PEKVCDFILRVREFQAKEPGDELATGSNASDDGMRIVLEDEPND
                     AVESELREFIRGLNVDEQIDLVALVWLGRGDGPREDWATLRAEAARQHDSRAAAPYLL
                     AMPLLADELADGLAQFGQTCGD"
BASE COUNT          528 a         1021 c         1069 g          560 t
ORIGIN      
        1 gaagcgaagc ggcgcagcgg ccatagcccg ctgcgcagag cttggtcggt tcgtccaacg
       61 gtcggtcggg ctatggccgc ttgacgatgc cgggccgatg tggccccgcg ccgagatgcg
      121 tgcatgccgt agccgcgcag cggggaaagg ggcggcaaag ccgccggtga gggctgcgct
      181 gaattttgtc ggttagcgat tcccaggcgc gtaccttgac agtcctgcgc cgcccctcat
      241 ccggcccttc gggccacctt ctccccgcaa agcggggaga agggatacga cgacggccga
      301 gcggaaatcg cgctaatccg ccgcgcgccc gacgggcgcc gcgatcctcg cggggccgcg
      361 caattcagcc gcatcgaacg tcgccgcctc gccgggcggc ggcaggagcg cgctcgatcc
      421 cggcaggccg ggatagaagg tcggatcggg ggcggcggct ttcgtcatgg cgagcacgcg
      481 cggcagcttc atcgcttctt cgaaggcatg atcggcgagg ctgaggatgg tcttggtgcc
      541 atgcggcgtc gcgccatcga aggtgaggtc atattcgagg ccgggcgcgc gttgcgtatc
      601 gacgcgcaga tagcggtcgc cgagatgatg gcgcaggaaa acctgcgtca attgttgctg
      661 cgccgacatg atcgtcgcga acaatcgtcc gccgcgcagc cagcgtgccg atccatagct
      721 gcggccatgg ctgtaaggca gcgaaaaatt accggtcgtc gtgccgatgg ataagacctc
      781 gatctcctcg accggcgtct tgagataata ttccgcctca tgcacgccga cgagatcggg
      841 cgcgttcgca aaaatcgcgc cgtcgacata gaggctgttg ctgagctcgg ccatcggaaa
      901 ataggtcggc gccgccgcgg cggccagggc gacctcgacc atcttgaagc cggaatgatg
      961 ggtccagttc gccagatgcg gcgtcttgat cacctcgatc tggccggtcg tcatattgac
     1021 gaccggcagc gccaaacggt ggcgcgccga ggccaaggtt ttgtcctcgt cgataaggct
     1081 ggcgatggct gcgcgcagcg ccgcgtcgga atatttgggc cgcagcagcg agcgcaggaa
     1141 ggcgagcgtg cgcttcgcct cgcggaacgg ccggttgatc tgcgggaaaa tgcgcgggcc
     1201 gtggtcgata aagacctgct cgatttcggc ggcgggaatt tccagcgaca gggcgagcgc
     1261 ggcgatcgcg cccgccgacg cgccgctgat caggtcgaag catcgggcga tcggcttgcc
     1321 ggccgccgct tcgagccgcg tcagaatgcg cgccgaatag aggccgagat agccgccgcc
     1381 gctcagcgca aggatgcgaa acgtcaccgc ggtcctccct gacctcgatg attctcgcgc
     1441 aaagattacc aggattttcg tccgccgggc aggcgctggc caaggaaact cggctcgcaa
     1501 aggcgagatt caggccgccc gctcaactcg ccttgcgagc cgctcggcgt ttcgccgcgc
     1561 cgatcttgcc ggcgcgcttg cgttttccgc cgccggcgcg gcccgcggcc ggcttctttg
     1621 cggatgcttt cgcagccggc gcgccggccg cggcggcgct tcgccgcagg gcgtccatca
     1681 gatcgaccac attggtcgac cgggcaggct tggcggcctc gatcggccgg ccttccagct
     1741 tggcctcgac cagttccgcc agcgccgctt cgtagcggtc gtcataggct ttcggatcga
     1801 agcggccggg cttcgtcttg atgatgtgct tggcgagatc gagcatctcc gattggattt
     1861 tgatcctggg aatgccggcg aacgcctcct cggccgagcg cacttcgtag tcgaaattca
     1921 gcagcgtcgc gatcatccct tcttcgtggg ggcgcatcag cacagtccgc accctgcgga
     1981 acaggacggc ccgggcgagc gccgcgactt tcgccttgcg catgccttcg cgaatgagcg
     2041 caaaagcgtg ctgcgaaacc ggttcggcgg ggccgagata gtagggacga tcgaaataga
     2101 gattgtcgat ttcgtcgcag gcgatgaaat gctccaccgc cagggtcttg tcgctgtccg
     2161 gaaaggccga cgcgatctcc tcgggctcga tgatcacata ttcgcccttg ctcgcctcgt
     2221 agcctttgac ctgatcgtcc gattcgaccg gcttgccggc gccctgatcg acaaattgcc
     2281 gatggacccg atcgccggtc ttgcggttga gcatgtggaa agcgatgcgc tcggacgtcg
     2341 aagccgcggt atagagagcg accggacagg tcaaatcgcc gatcctgatc actcccttcc
     2401 aattggcgcg aggcggcatg cggctcccca aaaaggccgt aacgcgcgag gccgagtctg
     2461 gttccggcgg cgcgccttgc gcgccttccc gccggaactt tctttttcgc cggccggttg
     2521 agcgcgaacc gaaggagcgg cgcagacggc cgcgcggaga cttccatggc gagaatttcc
     2581 tacaccgtcg cgcccactgc cgacgaatgg tccgtctttc gcgacggcga gcgtgggatg
     2641 agctacgcga cccaggaagc cgccttcgaa gtcgcgatcg ccgaggcttc cggcgacctg
     2701 cgatccggcc acgaggtggt gattgaagtc cgcgcggctt tcgccgaccc ggtcgatcgc
     2761 cggcccagcg agcggctata ggcgagagca actcgcggct ttcaatcgcc gcatgtttgg
     2821 ccgaattggg cgagcccgtc ggcgagctca tcggcaagca gcggcatggc gagcagatag
     2881 ggcgcggcgg cgcggctatc gtgctggcgg gcggcctccg cccttagcgt cgcccagtct
     2941 tcccgcggcc cgtcgccgcg gccgagccaa accaaggcga cgaggtcgat ctgctcgtcg
     3001 acattcaggc cgcggatgaa ttcgcgaagc tcggattcga cagcgtcatt gggctcgtct
     3061 tcgaggacga tccgcatccc gtcatcgctg gcgttcgatc ctgtcgccaa ctcgtcgccg
     3121 ggttctttgg cctggaattc ccgcacgcgc agaatgaagt cgcagacttt ctccggcg
//
LOCUS       DATGJW010000107         2465 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_641956, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000107 DATGJW010000000
VERSION     DATGJW010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2465)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2465)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2465
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_641956"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VKU03_03125"
     CDS             <1..365
                     /locus_tag="VKU03_03125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLJ70282.1"
                     /translation="AAVAALHARAKRPEDTDWAAIDALYAALERLQPSPVVTLNRAVA
                     TGKIHGPEAALAMIEPLGSRLSGYFHYFGAKGAFLLQLDRKDEARAAFNQAIALAADA
                     TQAAAIRRHLDRLIRDAV"
     gene            536..955
                     /locus_tag="VKU03_03130"
     CDS             536..955
                     /locus_tag="VKU03_03130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004124873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxalase/bleomycin resistance/extradiol
                     dioxygenase family protein"
                     /protein_id="HLJ70283.1"
                     /translation="MAASAEKSQSPNVPVKGGVVAYINVDGATKAAEFYKRAFNAEVA
                     FAYPPDNKGRTMHIHLYINNGSLMLSDFYPEHGYAVVKPQGFTLTLNVSDIDAWWRRA
                     TEAGCEAVMPLADMFWGARYGQLRDPFGVLWALNQPK"
     gene            1275..1505
                     /locus_tag="VKU03_03135"
     CDS             1275..1505
                     /locus_tag="VKU03_03135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70284.1"
                     /translation="MGSKRVYTAQAVGAKKNWTDREWQIYGDLPWIMQKFVDIVIRVP
                     RFVRSAAPGKVDRRSRFVERKLLEARRQAHKG"
     gene            complement(1554..1850)
                     /locus_tag="VKU03_03140"
     CDS             complement(1554..1850)
                     /locus_tag="VKU03_03140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HLJ70285.1"
                     /translation="MAERVLRLLRIRGRVQGVGYRAFVHEQATRLKLCGWARNRRDGS
                     VEVLVSGNASAAEALIALLRRGPPGARVADIMEDNAALDALAGYGDSFGILPTL"
     gene            complement(1865..2341)
                     /locus_tag="VKU03_03145"
     CDS             complement(1865..2341)
                     /locus_tag="VKU03_03145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase family protein"
                     /protein_id="HLJ70286.1"
                     /translation="MPLYFAYGSNIDAAAMARRCPRSAAVGPARLVRHRLAAMREGWL
                     TVVRDPRQSVHGILWNLALADMPALDRYEGVASGLYRKALQSVATESGAKRALVYFGA
                     NAGPGAAAPDYIAAIVAAARQWGLPVAALEAFLPGGASATEEPKEIRVRPRFPFDR"
BASE COUNT          454 a          830 c          779 g          402 t
ORIGIN      
        1 aggcggccgt cgccgcgctt cacgcgcgcg ccaaacggcc ggaggacacc gattgggcgg
       61 cgatcgacgc gctctacgcc gcgctcgagc ggctgcagcc ctcgccggtc gtcaccctca
      121 accgggccgt cgcgaccggc aagatccacg gaccggaagc cgcattggcg atgatcgagc
      181 cattgggctc tcgcctctcc ggctatttcc attatttcgg cgccaagggc gcgtttctct
      241 tgcagctcga ccgcaaggac gaggcgcgcg ccgcgttcaa ccaggcgatc gcgctcgccg
      301 ccgacgcgac gcaagccgcc gcgatccgcc gccatctcga ccgcctgatc cgcgacgccg
      361 tgtagccgcg gtcgccgacc gcggcgagaa ccgcgcttag ccgcggtcgc cgaccgcggc
      421 tccaattgcc ggcgtccggc gggaaatttc aaagcagaat gtcggcgatt ctcgccctca
      481 gccgtcctta ggccggaagc gagacatcgc tcgccaaacc agaaaggacc ccgtcatggc
      541 cgcaagcgcc gagaagtcgc aatctccgaa cgtccccgtc aaaggcggcg tcgtcgccta
      601 catcaatgtg gacggcgcga cgaaggccgc cgaattctat aagcgcgctt tcaatgcgga
      661 agtcgccttc gcctatccgc ccgacaacaa gggccggacc atgcatattc atctctacat
      721 caataacggc tcgctgatgt tgagcgactt ctatccggag cacggctacg ccgtggtcaa
      781 gccgcagggc ttcacgctga ccctgaatgt cagcgacatc gacgcctggt ggcggcgcgc
      841 gaccgaggcc ggctgcgaag ccgtcatgcc gctcgccgac atgttctggg gcgcgcgcta
      901 cggccagctt cgcgatccgt tcggcgtcct ttgggccctg aaccagccga agtgaacggc
      961 gccgccggta gccgcggtcg ttgaccgcgg ctacaatcct aagatcacgc gccgttacaa
     1021 aattttacat agccttccct tacgtcaaat cagcgcttta ggccgttcgc ttcaatcgcg
     1081 cattcaagcc cggagcggcg tgacacgaaa ccggctgaat ttagcctttt ttccaggaag
     1141 gattgcgccc gcgatttgcc ctggcgtaag cgttgaagtg cggcgtgagt tgatgcgttc
     1201 aaagcaggcg taatcgtcaa tgtacaaaat tcaggggcaa gcgtcagacc tcaatggcgt
     1261 tcagggagca agccatggga tcgaaacgcg tttatacggc gcaagcggtc ggagccaaga
     1321 aaaactggac cgaccgcgaa tggcagattt atggggattt gccttggatc atgcagaagt
     1381 ttgtcgacat cgtcattcgc gttccgcgtt ttgtgagatc agccgcgccg ggcaaggtcg
     1441 atcggcgatc gagattcgtc gaacgcaaat tgttggaggc gaggcggcag gcccataaag
     1501 gctagcgaac gcttcgcgcg gacggccggg acgcatgcgc ccggccgttt cttttataaa
     1561 gtcgggagga tgccgaaaga gtcgccatag ccggccagcg cgtcgagcgc ggcgttgtct
     1621 tccatgatgt cggcgacgcg cgcgccgggc ggcccgcgcc gcagcaacgc gatcaaggct
     1681 tccgccgccg aggcgttgcc gctgaccagg acttcgaccg agccgtcgcg gcggttgcgc
     1741 gcccagccgc aaagcttcag ccgcgtcgcc tgctcgtgga cgaaggcgcg gtagccgacg
     1801 ccttgcacgc ggccgcgaat gcgcagcaga cgaaggaccc gctcggccaa atttcgttct
     1861 cctttcaacg gtcgaaaggg aagcgcggcc gcacgcgaat ttctttgggc tcctccgtcg
     1921 cgcttgcgcc gcccggcagg aacgcctcga gcgccgcgac cggcaggccc cattgccgcg
     1981 ccgcggcgac gatcgccgcg atataatccg gcgccgcggc gccgggtccc gcattggcgc
     2041 cgaaatagac cagcgcccgc ttcgcgccgg attcggtcgc gacgctttgc agcgccttgc
     2101 gatagagccc gctggcgacg ccctcatagc gatcgagcgc cggcatatcg gcgagcgcga
     2161 gattccaaag aattccatgc acgctctggc gcggatcgcg cacgaccgtc agccatccct
     2221 cgcgcatcgc cgccagccga tgccgaacca gccgcgccgg cccaaccgcc gccgagcgcg
     2281 gacagcgcct ggccatcgcg gcggcgtcga tgttggagcc gtaggcgaag tagaggggca
     2341 tggcacatca ccgccgtctt atcctccccc gcgaaagcgg gggaggggga ccatgcgaag
     2401 catggtggag ggggcctccg gtctatcagg ataaatccca accgccggcg gccccctcca
     2461 ccgcg
//
LOCUS       DATGJW010000108         2080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_331504, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000108 DATGJW010000000
VERSION     DATGJW010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2080
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_331504"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..1452)
                     /locus_tag="VKU03_03150"
     CDS             complement(85..1452)
                     /locus_tag="VKU03_03150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_function: GO:0042910 - xenobiotic transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MATE family efflux transporter"
                     /protein_id="HLJ70287.1"
                     /translation="MSAVSAAPRVPRAVFTEGSTLRHVTVMTATGTVGLMAIFVVDLL
                     SLFYVSRLGQEALKAAIGYASQVLFLSVAINIGLTIAISATTARALGAGDRPRAQQLA
                     ASGLVIAGIVAALVAATLFTLRGAILDHVMHAQEPARDVADKFLAITLPANVPFAIGM
                     ACSGLLRAVGDARRAMYVTLTGGLVTAFTDPLLIFGFGLGVYGAAWATVVSRLVFLSV
                     GVYGAIYVHGLVGRPARKAMLRDFVPIMGIGGPAILANLATPAAAVYTTRVFSDFGEA
                     AVAAVAITDRIIPVAFGVIFALTGSVGPVLSQNLGARLMGRVRRALTESFLLSVAYVL
                     AAWALLFLATPFIVFAFKAQGDSARFLSFFCALGVSAWLFIACLFVANTAFNNLGFPV
                     LATAFNWGRATLGTIPFVTIGAQRWGVEGGMMGAVAGSAVFGVAAVATAYWVTARLAE
                     RIKTA"
     gene            1705..>2080
                     /gene="ispH"
                     /locus_tag="VKU03_03155"
                     /gene_synonym="lytB"
     CDS             1705..>2080
                     /gene="ispH"
                     /locus_tag="VKU03_03155"
                     /gene_synonym="lytB"
                     /EC_number="1.17.7.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186573.1"
                     /note="catalyzes the conversion of
                     1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into
                     isopentenyl diphosphate (IPP) and dimethylallyl
                     diphosphate (DMAPP); functions in the nonmevalonate
                     isoprenoid biosynthesis pathway; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-3-methylbut-2-enyl diphosphate
                     reductase"
                     /protein_id="HLJ70288.1"
                     /translation="MPQKPKLRIVLCAPRGFCAGVVRAIDVVERALALYGPPVYVRHE
                     IVHNRYVVESLKKKGAIFIEDLDEAPNSPAPVIFSAHGVPKSVPAEAEARGLVTIDAT
                     CPLVTKVHREAQVHFKRGRKIIL"
BASE COUNT          405 a          695 c          691 g          289 t
ORIGIN      
        1 ccctgcgccg cctgcatgat ctcgttgagg ttcatccgcc gtcctcccct cttaaagccg
       61 cttcgcgcgg cggccggaga aagtttaggc cgttttgatc cgctcggcga gccttgccgt
      121 cacccaatag gcggtcgcca ccgccgcgac gccgaaaacc gccgagccgg cgaccgcgcc
      181 catcatgccg ccctcgacgc cccagcgctg cgcgccgatc gtcacgaagg gaatggtccc
      241 caatgtcgcc cggccccagt tgaaggccgt cgccaggacc ggaaagccga gattgttgaa
      301 cgccgtattg gcgacgaaca ggcaggcgat gaagagccag gccgagacgc cgagggcgca
      361 gaagaaggac aggaaacgcg cgctgtcgcc ctgcgccttg aaggcgaaga cgataaacgg
      421 cgtcgccagg aacaggagcg cccaggcggc gaggacatag gcgaccgaaa gcagaaagga
      481 ttcggtcaac gcccgccgca cccggcccat cagcctggcg ccgagatttt ggctgagcac
      541 cgggccgacc gagccggtca gcgcgaaaat caccccgaag gcgaccggaa tgatgcggtc
      601 ggtgatggcg accgcggcga ccgccgcctc gccgaaatcg gagaagaccc gcgtcgtgta
      661 gaccgctgcg gccggcgtcg ccagattggc gaggatcgcc gggccgccta tgcccatgat
      721 cgggacaaag tcgcggagca tcgccttgcg ggcggggcgg ccgaccaggc catggacgta
      781 aatcgcgcca tagacgccga cgctgagaaa gaccaggcgc gacaccacgg tcgcccaggc
      841 cgcgccatag acgccgagcc cgaagccgaa aatcagcagc gggtcggtaa aggcggtcac
      901 cagcccgccg gtcagcgtta catacatggc gcggcgcgcg tcgccgacgg cgcgcaacag
      961 accggaacag gccatgccga ttgcaaaggg gacattggcc ggcagcgtga tcgccaggaa
     1021 cttgtcggcg acgtcgcgcg ccggctcctg cgcatgcatg acatggtcga gaatggcgcc
     1081 gcgcaaggta aacagcgtcg cggcgacgag cgccgcgacg atcccggcga tgaccagtcc
     1141 ggaggcggcc aattgctgcg cccgcggccg atcgccggcg ccgagcgcgc gcgccgtcgt
     1201 ggcgctgatc gcgatggtca aaccgatatt gatcgccacc gacagaaaga gaacctggct
     1261 ggcgtagccg atcgccgctt tcaaggcctc ctggcccaat cgggagacgt aaaagaggga
     1321 gaggaggtcg acgacgaaaa tcgccatcag gccgaccgtc ccggtcgcgg tcatcaccgt
     1381 tacgtggcgc agcgtcgagc cttccgtgaa aacggcccgc ggaacccggg gagcggcgga
     1441 aacggcggac acgcaaagct cctgtcgcgc cgggccggcg cggatagatt acggaatcgg
     1501 actgcccggc tgattttccc ctcgcgccgg gctggcgcgc gccttcaaga gttaatcggg
     1561 aacggaacgc tcgcttcgtc gggggcgtcg cttttaaggc cgccgcccaa atttcgccag
     1621 cgcaaaaagc ttccctgtcg gcggcctcgc ccttgacttt cagcgagcgg cctctgaaac
     1681 aacgcagcct cacccggtct ggccatgccg caaaaaccca agcttcgcat tgtcctctgc
     1741 gcgccgcgcg gcttttgcgc cggggtggtg cgggcgatcg acgtggtcga gcgcgcgctc
     1801 gcgctctatg ggccgcccgt ctatgtgcgc catgaaatcg tgcataacag gtatgttgtc
     1861 gaatccctga agaagaaggg cgccatcttc atcgaggatc tcgacgaggc gccgaactct
     1921 cccgcgccgg tcatcttctc ggcccatggc gtgccgaaaa gcgtgccggc ggaggccgag
     1981 gcgcgcggcc ttgtcacgat cgacgccacc tgcccgctgg tgacgaaagt acatcgcgag
     2041 gcgcaggtcc atttcaagcg cggccgcaag atcatcctgg
//
LOCUS       DATGJW010000109         4682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1264529, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000109 DATGJW010000000
VERSION     DATGJW010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4682
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1264529"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..1385
                     /locus_tag="VKU03_03160"
     CDS             27..1385
                     /locus_tag="VKU03_03160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MotB family protein"
                     /protein_id="HLJ70289.1"
                     /translation="MTAPHPEIIVVKRHGPQEDGHHGGAWKIAFADFMTAMMAFFLVL
                     WIISATDKNTKTLIARYFNPVKLEEPARAQKGIHGVPVKDAPYAPGEETASPSAGKPS
                     AETGEASGKADQNPASPPADNGQARPPTPSPTPPPDPAKPYPTMSEQELFSDPRASLD
                     KIAGAPPPGPRIDPSAAMKGYGEVGPSADEALRDPFRPLGSDRAVNVTAHDPGAPPAA
                     AAPSDEPVSMSTSIAPAAGSPGGSAEAQDETSPSPPAPAEAQGAGPQAAPAEGPAQSQ
                     GAAPPAPSQPVSPAPDAAQARQTAAANLLAEVKKRLAGEMRSTQGPELDVQATEDGVL
                     ISLTDRDNFSMFAIGSAEPQPRVVKMMAAIAASLRAMPGPIIVRGHTDARRYRSATYD
                     NWRLSSARAQMAYYMLIRGGLAEKRVERVEGYADHRLKDPSHPLAAENRRIEILVREA
                     KP"
     gene            1382..2584
                     /locus_tag="VKU03_03165"
     CDS             1382..2584
                     /locus_tag="VKU03_03165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70290.1"
                     /translation="MIGRAFTRPGLALALAAFALLAPSAASADSAKVSDLVDDLRRMQ
                     LRIAQGDKAAYAAELNQLKTIGAAIATASPETWKDKREADSLVIYILSGGTLAAVEPL
                     LRGDAVIESERSLARGALAYVTNHETEAAALLKETSLTALDARLAGEVAFARSVVETK
                     RNAKAAIELLDWARLLSPGGLVEEAALRREIALLAEARDAGRAAMLIRQYVTRFGSSL
                     YAAEFLRDLAGAVARLGLADDAANYKLVSGAIAALPPDGRRDFLLNMARSAVVSAKFA
                     AAAAAATEALESSRADSPEAMRARLYLATSRLFSDAYDAAIADLQSLSASQLDRADLG
                     ILAAARRVAAELRIAPDLSVFNTRNAAALPDPDKDKPAGPALTIAKAEEALQRTAGLA
                     GLQNGGSR"
     gene            2581..3894
                     /locus_tag="VKU03_03170"
     CDS             2581..3894
                     /locus_tag="VKU03_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HLJ70291.1"
                     /translation="MTAPAAAPASGPIVPPPPRAALQPPADAHAFAAVLDSLPGGEVK
                     PGPSTAEEKPRPSGEKRLDPSPGQKPGHSLPGDGSLLASLPFALQAASTIDEGPADHT
                     PSLGPPAAKAPEPEVSGASLAATARTAALGRLVGERAFHFGASGSATRQSGGALAIDW
                     PFASAASSAPVASFAPEANPSGGPALAASLPAAGAAPIAPRPGKAAANRVGPGRPANE
                     AARSGPGQEAAAPPPAERIAASAATRTPAESSGDGKAPGARSPDPAASAAPSLAQAGP
                     FGAPLSAPFGAAGPFAADGSAAGGADIAPSVGAPVPSPASTAPPVREIDVDLSPGGLE
                     DVSMTMRLAGDRLSVVVRAASSQTLSSIEGARDAIADRLAAIGQPLDSLIIKQTGVRA
                     DGTTGNATSADDGSAGDTWRPAQGAGERGGNDALSRRGAHRDRSF"
     gene            3851..4405
                     /locus_tag="VKU03_03175"
     CDS             3851..4405
                     /locus_tag="VKU03_03175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333026.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLJ70292.1"
                     /translation="MRCLGAALIAIAAFSGAARAEGPLACEREMTRAAALHGVPLNVL
                     YSVGLTETGHRGELSPYDMNVDGQAVHSATLAQAMARFAAAKARGAKFIDIGCMQINQ
                     RFHGAEFRSLSDMFDPVRNVEYAANFLKALKAQEGSWTLAVARYNAGPGNPAAERTYV
                     CSVIRNMIASGFGRWTANARALCQ"
     gene            complement(4500..>4682)
                     /locus_tag="VKU03_03180"
     CDS             complement(4500..>4682)
                     /locus_tag="VKU03_03180"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70293.1"
                     /translation="IPGKFQGNSREIPVTGNSSDILLNVELARTEVTIFSKVSPYCCT
                     VLLRCIVVVANGAASG"
BASE COUNT          728 a         1697 c         1594 g          663 t
ORIGIN      
        1 tgagttcgag gcctaagcaa gagccgatga ccgctcccca tccggaaatc atcgtcgtca
       61 agcgccacgg gccccaggaa gacgggcatc acggcggcgc gtggaagatc gccttcgccg
      121 atttcatgac cgcgatgatg gccttttttc tcgtgctgtg gatcatcagc gcaaccgaca
      181 agaacaccaa gacgctgatc gcgcgctatt tcaatccggt gaaactggag gagccggcaa
      241 gggcgcaaaa gggcatccac ggcgttccgg tcaaggacgc cccctatgcg ccgggcgagg
      301 agaccgcctc gccgagcgcc gggaaaccct ccgccgagac cggcgaagcc agcgggaagg
      361 ccgaccaaaa cccggccagc ccgccggcgg acaacggaca ggcgcgcccg ccaaccccct
      421 cgccgacgcc gccgcccgac ccggccaagc cctacccgac catgtcggag caggaactgt
      481 tcagcgatcc tcgcgcgagc ctcgacaaga tcgccggcgc cccgccgccc gggccgcgca
      541 tcgacccttc ggcggcgatg aagggttacg gcgaagtggg gccgagcgcc gacgaggcgt
      601 tgcgcgaccc gttccgtccg ctgggcagcg accgggcggt caatgtgacc gcccacgatc
      661 ccggcgcgcc gcccgcggcc gccgcgccca gcgacgaacc ggtttcgatg tcgacgtcga
      721 tcgcgccggc ggccgggtcg ccgggaggct ccgccgaagc gcaagacgag acatcgccgt
      781 ctccgccggc gccggcggaa gcccagggcg cgggaccgca ggccgcgccg gctgaaggcc
      841 cggcccaatc gcaaggcgcg gcgccgccgg ctccgtcgca gcccgtttcc ccggcgcctg
      901 acgccgctca agcccgccaa acggccgcgg ccaacctgct ggccgaggtg aagaagcggc
      961 tggccggcga aatgaggtcg acgcagggcc ccgagctcga tgtccaggcg accgaggatg
     1021 gcgtcctcat cagcctcacc gaccgggaca acttctccat gttcgccatc ggctccgccg
     1081 agccgcagcc gagggtcgtg aagatgatgg ccgcgatcgc cgcgagcctg cgagccatgc
     1141 cggggccgat catcgtgcgc ggccataccg acgcccgtcg ctatcggtcc gccacttatg
     1201 acaattggcg gctgtcctcg gcgcgggcgc aaatggccta ttacatgctg attcgcggcg
     1261 ggctcgcgga aaagcgggtt gagcgggtcg agggctacgc cgaccatcgt ctcaaggacc
     1321 cgtctcatcc gctcgcggcg gaaaaccgcc ggatcgagat tctggtgcgc gaggccaagc
     1381 cttgatcggt cgagccttca cccggcccgg gcttgcactg gcgctggccg cgttcgccct
     1441 gctcgcgcct tccgcggcat ccgccgattc ggccaaagtc tccgacctcg tcgacgatct
     1501 gcggcggatg cagctcagga tcgcccaggg cgacaaggcg gcctatgcgg ccgagctcaa
     1561 tcagctgaag acaatcggcg cggcgatcgc gacagccagt cccgagacct ggaaggacaa
     1621 gcgcgaagcc gattcgctgg tcatctacat cctgagcggc gggactctgg cggcggtcga
     1681 gccgctgctc aggggcgacg cggtcatcga gtcggagcgg tcgctggcgc gcggcgcgct
     1741 cgcctatgtc accaatcacg agaccgaggc cgccgctctg ctcaaggaaa cgagtttgac
     1801 cgcgctcgac gcgcggcttg cgggcgaggt cgcgttcgcc cgctcggtcg tcgagaccaa
     1861 gcgaaacgcc aaagccgcga tcgagcttct ggattgggcg cgcctgcttt cgccgggggg
     1921 actggtcgag gaagcggcgc tcaggcgcga gatcgccctc ctcgcggagg ccagggacgc
     1981 gggccgcgcg gcgatgctga tccgccaata cgtgacccgt ttcggctctt cgctctacgc
     2041 ggcggagttc ctgcgcgatc tggcgggcgc cgtcgcgcgg cttggcctcg ccgacgacgc
     2101 cgccaattat aagctcgtgt cgggcgcgat tgccgcgctg ccccccgacg gccggcggga
     2161 cttcctgctc aatatggcca ggtccgccgt cgtcagcgcg aaattcgccg ccgcggcggc
     2221 cgcggcgacc gaggctctcg aaagctcgcg cgcggacagc cccgaggcca tgcgcgcccg
     2281 cctctatctc gcgaccagcc gcctgttctc cgacgcctac gatgcggcga tcgccgattt
     2341 gcagagtctg tccgcttcgc aactggaccg ggccgacctg ggcatcctgg ccgcggcccg
     2401 gcgggtggcg gcggagttga ggatcgctcc cgatctcagt gtcttcaaca cgcgcaacgc
     2461 cgccgccttg cccgatcccg acaaggacaa gccggccggc cccgcgctca cgatcgcgaa
     2521 ggccgaggag gcgcttcagc gcaccgccgg cctcgcgggg ttgcagaatg gaggttcgcg
     2581 atgaccgccc cggccgccgc gcccgcttcg gggccgatcg ttccgccgcc gccgcgcgcc
     2641 gctctccagc cgccggccga cgcccatgcc ttcgccgccg tgctcgactc gcttcctggc
     2701 ggcgaggtga aaccgggccc ctcgacggcc gaggagaagc cgcgcccttc aggcgagaag
     2761 cggctggacc cgtcgcccgg acagaagccc ggccattccc tgcccggcga cggctcgctg
     2821 ctggcgtctt tgccgttcgc cttgcaggcc gcctcgacga tcgacgaggg tcccgcggac
     2881 catacgcctt ccctcggccc cccggcggcg aaagcgccgg aacccgaggt cagcggcgct
     2941 tcccttgccg caaccgccag gacggcggct ctcgggcggc tggtcggcga acgcgcgttt
     3001 cacttcggcg cctccggctc cgccacaagg cagtccggcg gcgcgctcgc gatcgattgg
     3061 ccgttcgcgt ccgccgcctc cagcgccccc gtcgccagct ttgcgccgga ggccaatccg
     3121 agcggcggac ccgcgctcgc cgccagtctg cccgcggccg gggccgcgcc gatcgcgccc
     3181 cgtccgggca aggccgcagc gaatcgcgtc ggccccggtc ggccggccaa tgaggccgcg
     3241 cgaagcggac cagggcaaga ggccgcggcg ccgcctccgg ccgaacggat tgcggcttcg
     3301 gccgcgaccc gcacgccggc ggaatcgagc ggcgacggca aggcgccggg cgcccgctcg
     3361 cccgatccgg ccgcgtccgc ggcgccctcg ctcgcgcaag ccggcccgtt cggcgcgccg
     3421 ctctcggcgc cgttcggcgc ggcggggccg ttcgcggctg acgggtcggc cgcgggaggc
     3481 gcggacatcg cgcccagcgt tggcgcgcct gtccccagcc cggcgtcaac agcgccgccg
     3541 gtgcgagaga tcgacgtcga cctgtcgccg ggcggccttg aggacgtatc gatgaccatg
     3601 cggctcgcgg gcgacaggct ctccgtcgtc gtccgcgccg caagctctca gaccttaagt
     3661 tcaatcgaag gagcgcgcga tgcgatcgcc gatcgcctgg cggcgatcgg gcagccgctc
     3721 gactcgctca tcatcaagca gacgggcgtc agggccgatg gaacgactgg aaacgcgacc
     3781 tccgccgacg acggctcggc tggggacaca tggcggccgg cgcagggcgc gggcgagcgc
     3841 ggcgggaacg atgcgctgtc tcggcgcggc gctcatcgcg atcgcagctt ttagcggcgc
     3901 cgcgcgggct gaagggccgc tcgcctgcga gcgggaaatg acgcgcgccg cggcgctgca
     3961 cggcgttccg ctcaatgtgc tctattcggt tggcctgacc gagacgggcc acaggggcga
     4021 gctcagcccc tatgacatga atgtcgacgg gcaggcggtg cattccgcca cgctcgccca
     4081 ggcgatggcg cgattcgccg cggccaaggc gcgcggggcg aagttcatcg acatcggctg
     4141 catgcagatc aaccagcggt tccacggcgc cgagttccgc tccttgagcg acatgttcga
     4201 tccggtccgc aacgtcgaat atgccgcgaa cttcctgaag gcgctgaagg cccaggaagg
     4261 ttcgtggacg ctcgccgtcg cccgctacaa cgccgggccc ggcaatccgg cggcggagag
     4321 gacctatgtc tgctcggtca tccgcaacat gatcgcgagc ggcttcgggc gatggacggc
     4381 gaacgcccgc gcgctctgcc aatagaggcg cgcggagggc ttcctttttg gggagcgcca
     4441 gatcctgggg gttggccgcc gccgaggccc gaccctttcc gagtccgaat cgaggctttt
     4501 caaccgcttg cggcgccatt cgcgaccaca acaatacagc gcaacaatac ggtgcaacaa
     4561 tacggtgaca ctttactaaa tatcgtaact tctgttcgcg ccaattctac attgagtaaa
     4621 atgtcactgg aatttcctgt cactggaatt tccctggaat ttccctggaa tttccctgga
     4681 at
//
LOCUS       DATGJW010000110         3638 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1367716, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000110 DATGJW010000000
VERSION     DATGJW010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3638)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3638)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3638
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1367716"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VKU03_03185"
     CDS             complement(<1..606)
                     /locus_tag="VKU03_03185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007790104.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLJ70294.1"
                     /translation="MSNALLDHAARELAAIRDAGLMKRERAIASPQGGSVLVAEDSAK
                     TELLNLCANNYLGLADHPRLIQAAKSALDSHGFGMASVRFICGTQDLHLALEKKLAAF
                     LKSEDAILFSSCFDANGGIFEALLGEDDAIISDALNHASIIDGIRLCKARRLRYANNN
                     MEELEARLKEAAGAKARLIVTDGVFSMDGIIADLPAICALAE"
     gene            731..2023
                     /locus_tag="VKU03_03190"
     CDS             731..2023
                     /locus_tag="VKU03_03190"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609828.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006221 - pyrimidine nucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HLJ70295.1"
                     /translation="MADSRPIALVNARLIDPESGQEIRGGVLAAFGVIAGLGPDVRRN
                     SAPAEALVVDCQGDIVAPGLIDMRAFVGEPGAEHRETIATASAAAAAGGVTTIVARPD
                     TNPPVDDPAVVDFILRRARDTAKVRVLPSAAITKGLQGEEIAEIGLLGEAGAVAFTDG
                     RRSVANAQVMRRALTYARDFGALIMNYCEDGNLVGGGVMTEGEQASRLGLPGAPREAE
                     TIVLDRDLRLAALTRARYHAVFVSNSLSVEAMRRAKQAGLGATCGVSINHLTLNEIDI
                     GDYRTFLKLAPPLRSEDERRVLVEALAEGVIDVIVSDHDPQDVEVKRLPFGEAEAGAV
                     GVETMLSAGLRLVSAGAIALPRLLYAMSTRPAQILGLPGGRLKVGAPADLIRFDPDEP
                     YVLDPSSLRSHCRNTPFDEARMEGRVKLTLVAGKVAHE"
     gene            2147..2752
                     /gene="plsY"
                     /locus_tag="VKU03_03195"
     CDS             2147..2752
                     /gene="plsY"
                     /locus_tag="VKU03_03195"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HLJ70296.1"
                     /translation="MLFQAWPSDLSALVLGYVCGSIPFGLLLTRWAGAGDVRAIGSGN
                     IGATNVLRTGRRDLAAATLVLDALKGTFAVLVASYAFGRDAALAAAIGAFLGHVFPIW
                     LGFKGGKGVATFLGCLSGIAWPAALVFAAVWLVVAFATRYSSAAALAASLAAPIALYA
                     AFGRAPAALVFAALAVLLWIKHRANIERLLKGAETKIGRKG"
     gene            2755..>3638
                     /gene="dprA"
                     /locus_tag="VKU03_03200"
     CDS             2755..>3638
                     /gene="dprA"
                     /locus_tag="VKU03_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HLJ70297.1"
                     /translation="MAAEPTPIRLTDAQRFDWLRLIRSENVGPRTFRSLVNSCGGARA
                     ALQALPELARRGGARAVRIASVKEIEQELEAARRIGARFVALGEADYPPLLRQIDSAP
                     PIISVRGAAEALQRPTVAVVGSRNASAAGLAFAERLTRGIGAAGYVVASGLARGIDQR
                     AHIASLETGAVAVLAGGLARPYPPEAAPLMEKIAAKGAAISEMPIEWEPRGRDFPRRN
                     RIVSGLALGVVVVEAARGSGSLITARFAAEQGRLVFAAPGSPLDPRAEGTNDLIRNGA
                     TICTKAGDVIADLEPMREG"
BASE COUNT          510 a         1200 c         1291 g          637 t
ORIGIN      
        1 ctcggcgagc gcgcagatgg ccggcagatc ggcgatgatc ccgtccatcg agaagacgcc
       61 gtcggtgacg atcagccgcg ccttcgcgcc ggccgcctct ttcagccgcg cttcgagctc
      121 ctccatatta ttattggcgt agcgcaggcg cctggccttg cagaggcgga ttccgtcgat
      181 gatcgaggcg tggttcagcg catcggaaat gatagcgtcg tcctcgccga gcagggcttc
      241 gaaaattccg ccattggcgt cgaagcagga ggagaagagg atcgcgtcct cgctcttcaa
      301 gaaggcggcg agcttctttt ccagcgccag atgcaggtcc tgcgtgccgc agatgaagcg
      361 caccgaggcc atgccgaagc cgtggctgtc gagcgcgctt ttggcggctt ggatcaggcg
      421 cgggtgatcg gcgaggccga gataattgtt ggcgcagaga ttgagcaact cggttttcgc
      481 cgagtcctcg gcgacgagga ccgagccgcc ttgcggcgag gcgatggcgc gctcgcgctt
      541 catcaggccg gcgtcgcgga tggcggcgag ctcgcgggcg gcgtggtcga ggagggcgtt
      601 ggacatggtc gctatatagc cgaaagggcg gcagtcggca gtcggcaatc ggcagtcgga
      661 cgcaacattt ccgccgccgg ctgccgactg ccgattgccg actgccgcgc cattgcgcta
      721 tggcgccctt atggccgaca gccgccccat cgcccttgtc aacgcccgtc tcatcgatcc
      781 cgaatccggg caggagattc ggggcggcgt tctggcggcg ttcggggtga tcgccggatt
      841 gggaccggac gttcgccgca actccgcgcc cgccgaagcg ctggtcgtcg attgccaagg
      901 cgacatcgtc gcgcctggcc ttatcgatat gcgcgcgttt gtcggcgagc ccggggcgga
      961 gcatcgcgag acgatcgcga cggcgagcgc ggccgcggcg gcgggcggcg tgacgacgat
     1021 cgtcgcgcgc cctgacacca atccgccggt cgacgatccg gcggtcgtcg atttcatcct
     1081 gcggcgcgct cgcgatacgg cgaaagttcg cgtgctgccc tcggccgcga tcaccaaggg
     1141 gctgcaaggc gaagagatcg ccgagatcgg cctgctcggc gaggccggcg cggtcgcctt
     1201 caccgatggg cggcgctcgg tcgccaacgc gcaggtgatg cgccgcgcct tgacctatgc
     1261 gcgcgatttc ggcgcgctga tcatgaatta ttgcgaggac ggcaatctcg tcggcggcgg
     1321 cgtgatgacg gaaggcgagc aggcgagccg gctcggcctg ccgggcgcgc cgcgagaggc
     1381 cgagacgatc gtgctcgatc gcgatttgcg cctcgcggcg ctgacgcgcg cgcgctatca
     1441 cgccgtcttc gtcagcaaca gcctgtcggt cgaggcgatg cgccgcgcta agcaggccgg
     1501 gctcggcgcg acatgcggcg tttcgatcaa tcatctgacg ctcaacgaaa tcgatatcgg
     1561 cgactaccgc acatttttga agcttgcccc gcccttgcgc agcgaggacg agcgccgcgt
     1621 gctggtcgag gccttggcgg aaggcgtcat cgatgtgatc gtctccgatc acgacccgca
     1681 ggatgtcgaa gtgaagcgct tgccgttcgg cgaagcggag gccggcgcgg tcggcgtcga
     1741 gaccatgctg tcggctggct tgcgcctggt ctcggcgggc gcgatcgctc tgccgcggct
     1801 gctttacgca atgtcaacgc ggcccgccca gatcctcggc ctgcccggcg ggcgtttgaa
     1861 ggtcggcgcg ccggctgacc ttatccgctt cgatccggac gagccttacg tgctcgatcc
     1921 gtcgtctttg cgctcgcatt gccgcaacac gccgttcgac gaagcgcgca tggaaggccg
     1981 cgtcaagctg actctggttg ccggaaaggt cgcgcacgaa taggctttgg cggaatggcc
     2041 gtcatggcga gcgaagcgaa gcaatccagc cgtagggcgc cgcccgtgga atggatcgct
     2101 tcgtcgcttc gctcctcgca atgacgggac ggggcggggc ggcggcgtgt tatttcaagc
     2161 ttggccaagc gatctctctg cgcttgttct cggctatgtc tgcggctcga tccctttcgg
     2221 cctgctgctg acgcgctggg ccggcgcggg cgatgtgcgc gccatcggct cgggcaatat
     2281 cggcgcgacc aatgtgctgc gcaccggccg ccgcgatctc gccgcggcga ctttggtcct
     2341 tgacgcgctg aaaggaacat tcgcggttct tgtggcctca tatgcgttcg gccgggatgc
     2401 ggcgctcgcc gccgcaatcg gggcgtttct cggccatgtc tttccgatct ggctcggctt
     2461 caaaggcggc aagggcgtcg cgacgtttct cggctgcctg agcggaatcg cctggccggc
     2521 ggcgctggtt ttcgccgcgg tttggctcgt cgtcgcgttc gcgacgcgct attcgtcggc
     2581 ggccgcgctg gcggcgagcc tggccgcgcc gatcgcgctt tacgccgcct tcggcagggc
     2641 gccggcggcc ttggttttcg ccgcgctcgc cgtcctgctc tggatcaagc atcgcgccaa
     2701 tatcgagcgg ctgctcaaag gcgccgagac caagatcggg cggaagggct gatcgtggcg
     2761 gccgagccga cgccgatccg gttgaccgac gcgcagcgct tcgactggct gcgtctgatc
     2821 cggagcgaga atgtcggacc gcgcaccttt cgctcgctgg ttaattcctg cggcggcgcg
     2881 cgcgccgctt tgcaggcgtt gcctgagctt gcgcgccgcg gcggcgcccg cgccgtccgc
     2941 atcgccagcg tcaaggagat cgagcaggaa ttggaggcgg cgcggcggat cggcgcgcgc
     3001 ttcgtcgcgc tcggcgaggc cgattatccg ccgctcctgc gacagatcga ttccgcgccg
     3061 ccgatcatct ccgtacgcgg cgccgccgag gcgctgcagc ggccgaccgt ggcggtcgtc
     3121 ggctcgcgca acgcctccgc cgcaggcctc gccttcgccg agcgcttgac gcgcgggatc
     3181 ggcgcggccg gttatgtcgt cgcttccggc ttggctcgcg ggatcgacca gcgcgcgcat
     3241 atcgcctcgc tcgagaccgg cgccgtcgcc gtgctggccg gcggtctcgc ccggccgtat
     3301 ccgccggaag ccgcgccgct gatggagaag atcgccgcca agggcgcggc gatctcggaa
     3361 atgccgatcg aatgggagcc gcgcggccgc gactttccgc gccgcaaccg gatcgtgtcg
     3421 ggcctcgcct tgggcgtggt cgtggtcgag gcggcgcgcg gatcgggctc gctgatcacc
     3481 gcgcgattcg ccgccgagca gggcaggctg gtcttcgccg cgcccggctc gccgctcgat
     3541 ccgcgcgcgg aagggaccaa cgatctcatt cgcaacggcg cgacgatttg caccaaagcc
     3601 ggagacgtca tcgccgatct cgagccgatg cgcgaagg
//
LOCUS       DATGJW010000111         4770 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_642374, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000111 DATGJW010000000
VERSION     DATGJW010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4770)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4770)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4770
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642374"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..835
                     /locus_tag="VKU03_03205"
     CDS             <1..835
                     /locus_tag="VKU03_03205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70298.1"
                     /translation="SGGAHGLAQSLFQVGGNFGQSLGPLLAAFVILPHGRASLAWFAL
                     AALGGMVILAALGNWSNRNGAARRPGRKAGAEPIALPKAQVARAIAVLIALIFSKYFY
                     LASITSYYIFYLMRRFDLPTQTAQVYLFVFLAAAAAGTVIGGPIGDRFGRKAVIWGSI
                     LGVLPFTLILPHVGLAATIALSVAIGLILSSAFSAIVVYAQELMPGRIGMVSGLFFGL
                     AFGMAGLGAAALGKLADWTSIEFVYQVCAFLPAIGLLAAFLPDTGGMQRACPRGATAD
                     D"
     gene            961..2601
                     /locus_tag="VKU03_03210"
     CDS             961..2601
                     /locus_tag="VKU03_03210"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_process: GO:0009966 - regulation of signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLJ70299.1"
                     /translation="MLDRRAEPRRRILKQAKIFFNRRRSVIDCLVRNVSETGACLEVA
                     NPVGTPDAFDLLIEGETAPRPCVSVWRHDSKIGVAFGEAAPGRTDEEAGARELKPIDA
                     EHPGAAGPVDDGADLVRGELLALRAALDEVETGVVLLDGALRAQFINRAFRAMWRLPD
                     AKARAKPSFAALMHHGTETGQYEIAPQDLSAYVAERIEQVAAGDPRPRDIRLSNGGVI
                     RFQCAALPSGGRMLSYTYVSDIAKQRDELVALRAALDNIDLGVILLDGDLNAQFINVA
                     ARRLGRISDAAADKRPAFAEILSDTRRNGAFALQGAELDALIALRVAQVRAGAPEPMD
                     LRAADGRIVRAQCAVLPNGGRMLTYGDVSDLVHQIDEREAQARIDPVTGIANRKHFLE
                     LAEVEWNRYCRYQRPLSLMMIDVDHFKTINDAFGRQAGDRILADIARVLQSVKRYSDA
                     LGRVGGEEFAVLLPETTLPQAKMAAERFRRAVAEQAVAVNGEPIKATVSIGVAEAWEA
                     AANIAALMNRADQALYLAKSAGRNRVETARPAPAFALG"
     gene            2949..3842
                     /locus_tag="VKU03_03215"
     CDS             2949..3842
                     /locus_tag="VKU03_03215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ70300.1"
                     /translation="MNSPLNWDEFRLVKAIADSRSLVGAAESLALNHSTVFRRLGAIE
                     AALGAQLFERSRAGYEPTAAGEEMIALATSMAESIIEFERHVAGRDVKPTGELRVTTV
                     DSVAVYLLGPILARFRSAYPGVHLDVILAAQNLNLSRRDADVAIRATNDPPETLVGRR
                     IAAIRWAVYCSPQLLAECGDVMKKAPFIGFGDNFSAHVGKRWMERTIGPKRQVWRVNS
                     VLSMAEAIAAGGGAGLLPCFVGDHRADLARFGAPLHELDIDLWILTHPDLRQAARVRA
                     FMDMVGADLAKARKVLEGAQA"
     gene            complement(4046..>4770)
                     /locus_tag="VKU03_03220"
     CDS             complement(4046..>4770)
                     /locus_tag="VKU03_03220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70301.1"
                     /translation="DGRRFSDDIDIFHDREESVATAAEADAALLAEEGFIVQWLRREP
                     GIFGAIVTRGKEQMKLEWVRDSDFRFFPTVPDDVFGSVLHTIDIATNKVLAAAGRREP
                     RDVLDLLFIHERHIPLAAVIWAAVAKDPGYSPESLIAEIRRNARYRADDYADLAVVTP
                     FDAASVAKRLKQILEEAELFARAMPAGKEGLVYTHNGAPVLPDPHRLADYVEHGGRRG
                     GHWPSSPEIGSAMLRDSAKPSS"
BASE COUNT          799 a         1533 c         1551 g          887 t
ORIGIN      
        1 ctccggcggg gcgcatggct tagctcagtc gctgttccag gtcggcggca atttcggtca
       61 atcgctcgga ccgctcctgg cggctttcgt catcctgccg catggccgcg ccagcctcgc
      121 ctggttcgcg ctcgccgcgc tcggcggcat ggtcattctc gcggcgctcg gcaattggtc
      181 gaatcgcaac ggtgcggcgc ggcggccggg gcgcaaggcc ggggcggagc caatcgcttt
      241 gccgaaagcg caggtggcgc gagcgatcgc ggtgctgatc gcgctgatct tctctaagta
      301 tttctatctc gcgagcatca ccagctacta cattttctac ctgatgcggc gcttcgatct
      361 gccgacgcag acggcgcagg tctacctttt cgttttcctc gccgccgcgg cggccggcac
      421 cgtgatcggc ggcccgatcg gcgatcgctt cggccgcaag gcggtgatct ggggctcgat
      481 tctcggcgtg ctgccgttca cgctgattct gccgcatgtc ggcctggcgg cgacgatcgc
      541 gctcagcgtc gcgatcggct tgatcctatc gtcggctttc tcggcgatcg tcgtctacgc
      601 gcaggagctg atgccgggcc ggatcggcat ggtctccggc ctcttcttcg gcctcgcttt
      661 cggcatggcc gggctcggcg cggcggcctt gggcaaactg gcggattgga cgagcatcga
      721 attcgtctat caggtttgcg cctttcttcc ggcgatcggc ttgctggccg cgtttctgcc
      781 cgacacggga ggcatgcagc gcgcttgtcc tcgcggcgcg acggcggacg actgagcagg
      841 tcgcgctcgc ggcaaagtgt tgccggtcgt tacaaattgc ggcgaaaact taagcggccg
      901 ggaacctctc cgaattcatg tttccgcatc ctcgctgcat ttttgccccg gaggcgctgg
      961 atgctggacc gtcgcgcgga gcctcgtcgc cggattttga agcaggccaa aatcttcttc
     1021 aatcggcgcc ggtcggtcat cgattgtctg gtgcgcaatg tgtccgaaac cggcgcctgc
     1081 ctggaagtgg ccaatccggt cgggacgccg gacgctttcg atcttttgat cgaaggcgaa
     1141 accgcgccgc ggccttgcgt ctcggtctgg cggcatgaca gcaaaatcgg cgtggctttc
     1201 ggcgaagcgg cgcctggccg tacggacgag gaggccgggg cgcgcgagct aaagccaatc
     1261 gacgccgagc atccgggcgc cgcaggccca gtcgacgacg gcgccgattt ggtgcggggc
     1321 gagcttctgg ccttgcgcgc cgcgctcgac gaggtcgaga ccggcgtcgt tctgctcgac
     1381 ggcgcgctca gggcgcagtt catcaatcgc gcctttcgcg caatgtggcg cctgcccgac
     1441 gccaaggcgc gggcgaagcc ttctttcgcc gccttgatgc atcacggcac cgagaccggc
     1501 cagtacgaga tcgcgccgca ggatctctcc gcctatgtgg ccgagcggat cgagcaggtc
     1561 gcggccggcg atccgcgccc gcgcgatatt cggctcagca atggcggcgt catccgcttt
     1621 caatgcgcgg cgctgcccag cggcggacga atgctgagct acacctatgt cagcgacatc
     1681 gccaagcagc gggacgagct cgtcgccttg cgcgcggcgc tcgacaatat cgatctcggc
     1741 gtcattctcc tcgatggcga tctcaatgcg caattcatca atgtggcggc gcgccggctg
     1801 gggcggattt ccgacgccgc cgccgacaag cggccggctt tcgccgaaat tctcagcgat
     1861 acgcgccgaa atggggcttt cgcgcttcaa ggcgccgagc tcgacgccct gatcgcgctg
     1921 cgcgtcgcgc aggtgcgcgc cggcgcgccg gagccgatgg acctgcgcgc cgccgatggg
     1981 cggatcgtcc gcgcgcaatg cgcggtcctc cccaatggcg gccgcatgct gacctatggc
     2041 gatgtcagcg atttggtcca tcaaatcgac gaacgcgaag cgcaggccag gatcgatcca
     2101 gtcaccggaa tcgccaatcg caagcatttc ctggagctcg ccgaagtcga atggaatcgc
     2161 tactgccgct atcagcgccc gctgtcgctg atgatgatcg acgtcgatca cttcaaaacg
     2221 atcaatgacg cctttgggcg gcaagccggc gatcgcattt tggccgacat cgcccgcgtg
     2281 cttcagagcg tcaagcgcta ttccgacgcg ctcggccggg tcggtggaga agaattcgcg
     2341 gtcttgctgc cggagacgac gctgccgcag gcgaaaatgg cggcggagcg attccgccgc
     2401 gcggtggcgg agcaggccgt cgccgtcaat ggcgagccga tcaaggcgac cgtcagcatc
     2461 ggcgtcgccg aggcctggga ggcggcggcc aatatcgccg cattgatgaa ccgagccgat
     2521 caggcgctgt atctcgccaa atcggccggg cgcaatcgcg tcgagacggc gcggccggcg
     2581 ccggctttcg ccctcggatg atcgggtgaa aagagaaagg ccgccccaaa ggggcggcct
     2641 ttccattgtc gcgaccgctt gagatcgctt cgaagcctta gatcaggcgc ggtctagccc
     2701 cgcacggaca ataatccagg tcttcgcgat ccgccgcggc gatggacaat gagacactgg
     2761 atcacctcct ttctgctggt tgacgaccca ctcagaatag gccgattcgt cgcgccgcaa
     2821 aggccagact gcgcaaggcg gccattatcc ccgatcttct tgaattgcag atgagacgcc
     2881 gacggcgctt gcaaatatgc cagcgagcgt ggcaagagac cgggccagcg gccgaggctt
     2941 cagacgccat gaattcgcct ttgaactggg acgagtttcg cttggtcaag gcaatcgccg
     3001 attcccgttc gctggtcggc gcggcggaat ccctcgcgct caatcactcc accgtgtttc
     3061 gccggctcgg cgcgatcgag gcggcgctgg gcgcgcagct cttcgagcgc tcgcgggccg
     3121 gctacgagcc gaccgcggcc ggcgaagaga tgatcgcgct ggcgacctcg atggcggaat
     3181 cgatcatcga attcgagcgc catgtcgccg gccgcgacgt caagccgacc ggcgaattgc
     3241 gcgtcaccac cgtcgattcg gtcgcggtct atctgctcgg cccgatcctg gcgcggtttc
     3301 gctcggccta tcccggcgtc catctcgacg tgatcctcgc cgcgcaaaat ctcaatttgt
     3361 cgcggcggga cgccgacgtg gccatccgcg cgaccaacga tccgcccgaa acgctggtcg
     3421 gacggcggat cgcggcgatc cgctgggccg tctattgcag ccctcagctt ctcgccgaat
     3481 gcggcgacgt catgaagaag gcgccgttca tcggcttcgg cgacaatttc agcgcccatg
     3541 tcgggaaacg ctggatggag cggacgatcg gcccgaagcg ccaagtctgg cgcgtcaatt
     3601 ccgtgctcag catggccgag gcgatcgccg ccggcggcgg cgccggcctg ctgccttgct
     3661 ttgtcggcga tcatcgcgcc gatctggcgc gcttcggcgc gccgttgcat gagctcgaca
     3721 tcgacctttg gattctgact catcccgacc tccgccaggc ggcgcgggtg cgcgccttca
     3781 tggacatggt cggcgccgat ctcgccaagg cgcggaaggt tttggagggc gctcaagcgt
     3841 gagtgaccat tcatcgggac ggagacaaat tttgatggag cgccagacat acctttctct
     3901 aaaaacgtcc cgctaaccag cgggatctga tgcggtaggc gcgcaccaca aatcgtgcag
     3961 cctgtcactc cgtacggtcg ggcttttcga gatttcgatg cgacctgaga aggcgcttgc
     4021 aaaccgagcg ggcaaggcgt tcgacttagg aagatggttt ggcgctgtct cgcagcatcg
     4081 cgctgccgat ttccggcgag gacggccaat gaccgccgcg ccggccgcca tgttcgacat
     4141 agtcggcgag tcgatgagga tcaggtagga ctggggcgcc gttatgcgta tagacgagcc
     4201 cctccttgcc tgcgggcatc gccctggcga agagttcggc ttcttccaaa atttgcttca
     4261 ggcgcttggc gacagaggcg gcgtcgaacg gcgtaacgac ggccaagtct gcgtagtcgt
     4321 cggctcggta acgcgcgttg cgacggatct ccgcaataag gctttccggc gaatagccag
     4381 ggtctttggc gacggctgcc caaatgacgg cggccaatgg gatatggcgc tcatggatga
     4441 aaagcaaatc gagcacgtcg cgcggctcgc gccggccggc cgccgccagt accttgtttg
     4501 ttgcgatatc gatcgtgtgc agcactgaac caaaaacgtc atcgggaacc gtgggaaaga
     4561 aacgaaaatc gctgtcgcgc acccattcga gcttcatctg ctccttgccg cgcgtgacaa
     4621 tggcgccgaa aatcccgggc tcgcgccgca gccactgtac gataaagcct tcctccgcga
     4681 gcaacgcagc atcagcctcg gcagccgtgg cgacactctc ctcgcggtcg tggaaaatat
     4741 cgatgtcgtc ggaaaagcgc cgcccatcgc
//
LOCUS       DATGJW010000112         5844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_539272, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000112 DATGJW010000000
VERSION     DATGJW010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5844
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_539272"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..644)
                     /locus_tag="VKU03_03225"
     CDS             complement(<1..644)
                     /locus_tag="VKU03_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HLJ70302.1"
                     /translation="MIDEKKLEERLVALEAAKSWSPRVVSRLETLLRSGADEALYRIN
                     PIQFAAEKSIAEAEGIDLFLHACVAGLFDMDWLLVCPMCSDVVESFRSLGKLHTHFHC
                     CLCQGDYDAALDDYITVTFTVSPAVRSIRFHKPEALSAWDYVFSYKMTPGGVTPDGVP
                     WSEAAKGLMRALTRIEPDSSANLAFDAAEGGLLGQDFDSDAQFFFTVASGAGATP"
     gene            complement(834..2003)
                     /gene="proB"
                     /locus_tag="VKU03_03230"
     CDS             complement(834..2003)
                     /gene="proB"
                     /locus_tag="VKU03_03230"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HLJ70303.1"
                     /translation="MRRFAPYPSVQPSPPAVTAPPNLARFNRIVVKVGSSLLVDPAKG
                     ALRQDWLDALADDLAALHKRGADVLVVSSGAIALGRTVAGLPKGALKLEDSQAAAAIG
                     QIALARAWAQALAARGVTAGQILVTLQDTEERRRYLNARATIARLLAMRAAPVINEND
                     TVATSEIRYGDNDRLAARVAAMASADLLVLLSDIDGLYDAPPSRNPHAKLIPVVERIT
                     PEIEAMAGGAASELSRGGMRTKIEAAHIAVNAGVHMAIADGRVTHPIRRIADKGRCTW
                     FLTPSNPLAARKIWIGGSLELKGAVYCDAGAVKALRDGKSLLPAGVARIEGAFSRGDC
                     VAIRDLDGLEIGRGLIAYDSLHAEQIKGRNSRDIAHILGMPGRAEMIHRDDMALR"
     gene            2230..2538
                     /locus_tag="VKU03_03235"
     CDS             2230..2538
                     /locus_tag="VKU03_03235"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004128342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative quinol monooxygenase"
                     /protein_id="HLJ70304.1"
                     /translation="MIRVVAIITAQPGRREQVLAAARAIVPAVRAERGCIEYQLVVDE
                     ADFGPVQTKLGPDVYAVIETWADADALKAHGVAPHMKAFGAETRDLIASRVIHVLTPA
                     "
     gene            complement(2929..3999)
                     /gene="obgE"
                     /locus_tag="VKU03_03240"
     CDS             complement(2929..3999)
                     /gene="obgE"
                     /locus_tag="VKU03_03240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HLJ70305.1"
                     /translation="MKFLDQAKIYIRSGDGGAGCVSFRREKFIEFGGPDGGDGGRGGD
                     VIAECADGLNTLIDFRYQQHFKAKTGMHGMGKNRAGGKGADIVLKVPAGTQIFDEDGE
                     TLIADLAKVGERVTLARGGNGGFGNAHFTTSTNRAPRRANPGQPGEERTLILRLKLIA
                     DSGLIGLPNAGKSTFLAAVSAAKPKIADYPFTTLHPGLGVVSIDGRDFVLADIPGLIE
                     GAHDGAGLGDRFLGHIERCRVLLHLIDATGENAGADYRTIRRELAAYGAGLAEKKEIV
                     ALSKIDAVDEETLKKQRDRLKRAIRAHGPAAAGEKRDAPLELSAVSGQGMKEALRALA
                     GAVYAGRAAAAAEQAPAAEWAP"
     gene            complement(4436..5044)
                     /locus_tag="VKU03_03245"
     CDS             complement(4436..5044)
                     /locus_tag="VKU03_03245"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ70306.1"
                     /translation="MFPDIARDDVFRLETSRLWLRWPRVADAPAIQRYSSKWDVARFT
                     ARIPHPYPAGEAERYIFAARQANAAGHDLILAIASNRGKREPFGMISVESRLQGRLTL
                     GYALAPEAWGKGYASEAARAIVDASFLLTHALEILATARIENPASRRVLEKCGFVHQG
                     VGREGAPARGGLVEVDRFKLERSVWAVDYGQRAAMVPPIDAA"
     gene            complement(5231..5497)
                     /gene="rpmA"
                     /locus_tag="VKU03_03250"
     CDS             complement(5231..5497)
                     /gene="rpmA"
                     /locus_tag="VKU03_03250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HLJ70307.1"
                     /translation="MAHKKAGGSSRNGRDSKGRRLGVKKFGGEQVAGGMILVRQRGTK
                     WHAGRNVGVGVDHTLFALTAGKVEFQTKANGRCYVAVKELQAAE"
     gene            complement(5587..>5844)
                     /gene="rplU"
                     /locus_tag="VKU03_03255"
     CDS             complement(5587..>5844)
                     /gene="rplU"
                     /locus_tag="VKU03_03255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HLJ70308.1"
                     /translation="ITVMTLAGEPGETVTFGEVLALFDGDTSHVGAPHIEGASVAGTI
                     VAQRRGPKVYAFKKRRRKNSRRKRGHKQDLTVVKITEIRAS"
BASE COUNT          949 a         1922 c         1946 g         1027 t
ORIGIN      
        1 ggcgtcgcgc ccgccccgct cgcgactgtg aaaaagaatt gcgcgtcgct gtcgaaatcc
       61 tggccgagaa gcccgccttc ggcggcgtca aacgcaagat tggccgaaga atccggctcg
      121 atgcgggtga gcgcccgcat cagccctttg gcggcctcgc tccatggtac gccgtcgggc
      181 gtgacgccgc ccggcgtcat tttgtaagag aagacgtaat cccaggccga aagcgcctcc
      241 ggtttatgga agcggatgct gcgcacggcg ggcgaaaccg taaacgtcac ggtgatgtaa
      301 tcgtcgagcg cggcgtcgta atcgccttgg cagaggcagc agtggaaatg cgtgtgcagt
      361 ttgcccaggc ttcggaagct ctcgacgacg tcggagcaca tcggacaaac gagcagccag
      421 tccatgtcga atagcccggc cacgcaggca tggagaaaaa gatcgatccc ttcggcttcg
      481 gcaatcgatt tctcggcggc gaactggatc ggattgatgc ggtagagcgc ctcgtccgcg
      541 cccgagcgca gcagcgtctc cagccgcgag acgacccgcg gactccagga tttggccgct
      601 tcgagcgcga ccaagcgctc ttcgagcttt ttttcatcga tcatcgcatg acgtccgcga
      661 gaaaaaccgc gatcattctc gtccgtccgt tcgcttcaat caacccggat gaaccagact
      721 atcggcatcg cccatgtcgg aattgggtca aagcaggacc tctttcttga ttttgacgag
      781 gccgcttcgg agggtctgac cttgcagctt ggatcgccga cggagatcgt acctcatcgc
      841 agcgccatat cgtcgcgatg gatcatctcc gcccggcccg gcatgccgag aatatgggcg
      901 atgtcgcgcg agttgcggcc tttgatctgc tcggcgtgca gcgaatcata ggcgatcagg
      961 ccgcggccga tttcaaggcc gtcgaggtcc cggatggcga cgcaatcgcc gcgcgagaag
     1021 gcgccttcga tccgggcgac gccggccggc aacaggctct tgccgtcgcg cagcgccttg
     1081 accgcgccgg cgtcgcaata gaccgcgcct ttcagctcca gcgagccgcc gatccagatt
     1141 ttgcgcgccg cgagcgggtt ggacggcgtc aggaaccagg tgcagcggcc cttgtcggcg
     1201 atgcgccgga tcggatgcgt cacccggccg tcggcgatcg ccatgtggac gccggcattg
     1261 accgcgatat gggcggcttc gattttggtg cgcatgccgc cgcgcgacaa ttcggaagcc
     1321 gcgccgccgg ccatcgcctc gatttccggc gtgatccgct cgacgaccgg aatgagcttg
     1381 gcgtgcggat tccggctcgg cggcgcgtcg tagagcccgt cgatgtcgga aagcaggacc
     1441 aggagatcgg cgctggccat ggcggcgacg cgcgcggcga ggcgatcatt gtcgccatag
     1501 cggatttcgc tggtcgccac cgtgtcgttc tcgttgatca ccggcgcggc gcgcatcgcg
     1561 agaagccgcg cgatcgtcgc ccgcgcgttg aggtagcggc ggcgctcctc ggtgtcctgc
     1621 aaagtgacga gaatctgccc ggcggtgacg ccgcgcgccg ccagcgcctg cgcccaggcc
     1681 cgggccagcg cgatctgccc gatcgccgcc gccgcctggc tgtcttcgag cttgagcgcg
     1741 cccttcggca ggccggcgac cgtccgcccg agcgcgatcg cgccggagga gacgacgagc
     1801 acgtccgcgc cgcgcttatg cagcgcggcg agatcgtccg ccagcgcatc gagccaatcc
     1861 tgtctcagcg cgcccttcgc cggatcgacg agaagcgacg acccgacttt gacgacgatg
     1921 cggttgaagc gggcgaggtt gggcggggcg gtcacggcgg gagggcttgg ttggacggac
     1981 ggatatggcg caaatcggcg catgtgggaa gcgggcgagg agcggccgcg tagctggtcg
     2041 tgccttcttt tttccttctc ggcgatgaat cgcccgtccg ccaagccgcg cggctgcgac
     2101 aacgcgtcgg aggcgaagag aagtcgcttc cattgcatcc ggcgttgaat cggcgcgctt
     2161 ttgcactttg aattgtcaaa acatcgcgta gaaaagtcga cgcatgccgc agctttcggg
     2221 agggttttta tgatccgcgt cgtcgcaatc atcaccgcgc agcctgggcg acgggaacag
     2281 gtccttgccg ccgcgcgcgc catcgtgccg gcggtgcgcg ccgagcgcgg ctgcatcgaa
     2341 taccagctcg tcgtcgacga ggcggatttc ggaccggtcc agaccaagct cggccccgac
     2401 gtctacgcgg tgatcgagac ctgggcggac gccgacgcgc tgaaggcgca tggcgtcgcg
     2461 ccgcatatga aggcgttcgg cgccgagacc agggatttga tcgcaagccg cgtcattcac
     2521 gtcctgacgc cggcttagcg ccgttccgcg caatttgacc tttacgatca tcaggttgcg
     2581 gaaaggcggc gaacattttc ttccccagcc gcgctgcctc ggctacaatg cgcgccgccg
     2641 acgggacaaa ggaacgcggg caggcggacg tacgtttgaa agcgcccgaa aaacgcgttt
     2701 ccaaagtttt cgcgctttag cctattgaaa tcgcttaaag cgaccaaaga aagttttgga
     2761 aaaatttggc gggcgcgttt cggcgcccct gacaagaaaa ggatttttgg gaacgccaat
     2821 ttgcggagcc ggcgtctttt taccgaatcc aaggattttc caaattttcg cgacgcgctc
     2881 tcggatcatg ccggagcggg cgaggttgtc tgaaggcgcc tctccgcctt atggcgccca
     2941 ttccgccgcc ggcgcctgtt ccgccgccgc agccgcgcgt cccgcgtaaa cggcgccggc
     3001 cagcgcccgc agcgcctctt tcattccctg ccccgacacc gccgaaagct cgagcggcgc
     3061 gtcgcgtttc tcgcccgccg ccgccggtcc gtgcgcgcgg atcgcgcgct tcagccggtc
     3121 gcgctgcttc ttcagcgtct cctcgtcgac ggcgtcgatc ttcgacagcg cgacgatctc
     3181 cttcttctcc gccaggccgg cgccatacgc ggcgagctcc cgccgtattg tgcggtagtc
     3241 ggcgccggcg ttctcgccgg tcgcgtcgat aagatgcagc agcacccggc agcgctcgat
     3301 atggccgagg aagcggtcgc cgaggccggc gccgtcatgg gcgccttcga tcaggccggg
     3361 aatgtcggcg aggacgaaat cgcggccgtc tatgctgacg acgccgaggc cgggatggag
     3421 cgtggtgaac ggatagtccg cgattttcgg cttggccgcc gaaaccgcgg cgagaaaggt
     3481 cgatttgccg gcgttgggca ggccgatcag gccggaatcg gcgatcagct tcaagcgcag
     3541 gatcagcgta cgttcttcgc ccggctggcc gggattggct ctgcgcggcg cgcggttggt
     3601 cgaagtcgtg aaatgggcgt tgccgaagcc gccattgccg ccgcgggcga gcgtgacgcg
     3661 ctcgccgacc ttggcgaggt cggcgatcag cgtctcgccg tcctcgtcga aaatctgcgt
     3721 gccggccggg actttgagca cgatgtcggc gcctttgccg ccggcgcggt tcttacccat
     3781 gccgtgcatg ccggtcttgg ccttgaaatg ctgctggtag cgaaagtcga tcagcgtgtt
     3841 gaggccgtcg gcgcattcgg cgatgacgtc gccgccgcgg ccgccgtcgc cgccgtccgg
     3901 gccgccgaat tcgatgaact tctcgcgccg gaacgagacg cagcccgcgc cgccgtcgcc
     3961 ggagcggatg tagattttgg cctggtcgag gaatttcatg atggacgggc ctcgtacctc
     4021 gccgggacgc gacggcgcgc tttcctcccc cgcggagcgg ctacggcatg cacacatctt
     4081 gaagcgcggc cgcatcggcc cggcatcgtc acccgaaggg gcgaagcgca gcggcgcagc
     4141 ggccatagcc cgctgcgccg agcttggtcg gttcgtccaa cggtcggtcg ggctatggcc
     4201 gcttgacgat gccgggccga tgtggccccg ccgcgccgag atgcgtgcat gccgtagcgc
     4261 ggagcggggg aggaggacca tgcgaagcat ggtggcgggg gcgcggcggt tgaagataaa
     4321 cccccagcgc cgcggccccc tccaccgccc ttcgggcggt ccgcctcccc cgcttcgcgg
     4381 gggaggaaat aggggctggc gccgcgccga gaattcataa cgcccatcgt catcatcacg
     4441 ccgcgtcgat cggcggcacc atcgcggcgc gctggccata gtccacggcc cagaccgaac
     4501 gctccagctt gaaccggtcg acctcgacca ggccgccgcg cgccggcgcg ccttcgcggc
     4561 cgacgccctg gtggacgaag ccgcattttt cgagcacgcg ccgcgacgcc gggttctcga
     4621 tccgcgccgt ggcgaggatt tcgagggcgt gagtcagcag gaagctcgca tcgacgatcg
     4681 cccgcgccgc ctcgctggcg tagcccttgc cccaggcttc cggggccagc gcgtagccga
     4741 gcgtcagccg gccttgcagc cggctctcga cgctgatcat gccgaacggc tcgcgcttgc
     4801 cgcggttcga ggcgatcgcc aggatcaggt cgtggccggc cgcgttggcc tggcgcgcgg
     4861 cgaaaatgta gcgctcggcc tcgcccgccg gatacggatg cggaatgcgc gcggtgaagc
     4921 gggcgacgtc ccatttgctc gaatagcgtt gaatggccgg agcgtcggcg acgcgcggcc
     4981 agcgaagcca cagccggctc gtctccaggc gaaagacgtc gtcgcgggcg atatcgggaa
     5041 acatggcgac tccagtccat cggggcccct gcccccagat gacgaagggg aggcgccagc
     5101 ctcccctttc aaagtcccgt cggggacctc gccggactgt cgaaggcttc tcgccttcgt
     5161 tgatcttggg cttgccgcca agaccgtccg cggggcttaa cgcctttgcg gggtctcgtc
     5221 ggcgccccgc tcattccgcc gcttggagtt ccttcactgc tacgtaacaa cggccattgg
     5281 ccttggtttg aaattcgact ttgccggccg taagggcgaa caaagtatgg tcgacgccga
     5341 ccccgacatt gcggccggca tgccatttgg tgccgcgttg tcgtacgaga atcatcccgc
     5401 ccgcgacctg ctcgccgccg aatttcttga cgccgaggcg gcgtcccttc gaatcgcgcc
     5461 cgttgcgcga ggaaccgccc gctttcttgt gggccatagc ctaaaaactc cggatgcttc
     5521 gctcgaaaga ctcaagcggc gcaaagctgc gcttctataa tccaaaaccg tgaataagga
     5581 aaccgcctag ctggcgcgga tctcggtgat tttcaccacc gtcaggtcct gcttgtggcc
     5641 gcgcttgcgg cgcgaattct tccggcgccg tttcttgaag gcgtagacct tggggccgcg
     5701 gcgctgcgcg acgatcgtcc cggcgacgct ggcgccttcg atatgcggcg cgccgacatg
     5761 gctcgtatcg ccgtcgaaca gcgccagaac ctcgccgaac gtcacagtct cgccgggctc
     5821 accggcgagg gtcatcacgg taat
//
LOCUS       DATGJW010000113        10900 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1368163, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000113 DATGJW010000000
VERSION     DATGJW010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10900)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10900)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10900
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368163"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..797)
                     /locus_tag="VKU03_03260"
     CDS             complement(<1..797)
                     /locus_tag="VKU03_03260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ70309.1"
                     /translation="MAAGTRFIESLHYVPEAGWTLSAFSVLRAGKVAAGPDYGIERAS
                     HTGQDILYCISGAGIVETLGQRLDMQSGQLAWIANEGPHAHRADPRAPWTLLWLRLDG
                     PNPAALRQKLFGDGPPRVAMPESANLSSWFDRLFVAMRRRELGLDLRLNHLVGEFLLI
                     VDRALSASDAAGAPQALTKIVNAMRRNLRRRWSAREIEALTELSPSQIRRLFHKYLRA
                     SPRQWLQRERLILAQSLITQSPAALAEIAETCGFCDVYHFSREFKRAV"
     gene            949..1716
                     /locus_tag="VKU03_03265"
     CDS             949..1716
                     /locus_tag="VKU03_03265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytanoyl-CoA dioxygenase family protein"
                     /protein_id="HLJ70310.1"
                     /translation="MTLAMTQEESARYARDGYILRRGLLGGDEVAKFRNHARAQLEKE
                     QRAGAVMTKGDKEGKATLLKMWTKAEDDQYGLLARDERMVDLAQDAIGKPIYLYSHKM
                     TMKQPHEGGAWEWHQDFGYWYNYGCLAPDMMSIYVALDKATRENGCLQVLKGSHKLGR
                     LNHIRENDQTNVEQEHLEAALKRFELVYVEMDAGDVLVFDGNLLHRSDANRTDTYRWG
                     YICSYNAVENAPFKRVREYGNYEELRKVPAGSFRMAS"
     gene            1839..2861
                     /locus_tag="VKU03_03270"
     CDS             1839..2861
                     /locus_tag="VKU03_03270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70311.1"
                     /translation="MADTARTGARAPAMDRFTPSRLLRAREAGVFCALLALCLFLTFA
                     TSGFLTSINLFNVGRQISLLGIMSVGMTFVLISGEVDLSVGSTYAFAGLTTGMLIIAK
                     WSLAPAIGAGVLAGAAIGLINGLLSTYGRLPSLIATLGMLSMARGAALIMTNGEPVTV
                     NVRNGAAPEVLAKFEFMGQGYLFGVVPMQLVFFAAVAAIAWAVLSYTNFGFRIFAVGG
                     SAKAARVSGINVSRVKISAFILMGGLAAFGGILSVAFLPSAQAGRTGLGLELDVIAAT
                     IVGGASLSGGEGTILGTILGVLIIGVMRNGLVLMGVSPFVQEFLIGLVIVVAVGIDKW
                     TTRRAA"
     gene            2890..3858
                     /locus_tag="VKU03_03275"
     CDS             2890..3858
                     /locus_tag="VKU03_03275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ70312.1"
                     /translation="MKLKHVFLAFAIVCAPFAAQAAQKLSDFPLADRIKAKIASGKPL
                     DIYVSYHDVSNEFAPSIKKGVQKAAADDKVDAQFIGPTGANADAQISEIETLMGKMDG
                     LAISSVSSDALAPVIDRVLKAGIPVVTFNTDNPNSKRLAFAGQDLVQSGREAGKLMVK
                     VLDGKGKVLITTLDAAAQWSLDREKGARESLKEAPGIEVVNTLNTGTDPQKIYSAIEN
                     AMLANPSITGILSLECCSTPAAGTWVQRNKAMGKVKVVGFDLLDQTVQLVQEGEIQAT
                     IDQAPEKQGFEAVDLLVNFLKGKPIDNVDTGVKIYTKENIAEVAKK"
     gene            3913..5421
                     /locus_tag="VKU03_03280"
     CDS             3913..5421
                     /locus_tag="VKU03_03280"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ70313.1"
                     /translation="MSRRNEPDLVEIAGVTKRFPGVLALDRVSISFRAGEVHAVIGEN
                     GAGKSTLMNILAGDLQPNDGEIRIDGRPVRFSSPLASRAAGIVVVYQELALCPTLSVA
                     ENIMMSEVASQPVVAFTPRAEMRRQARMALSRLGMASLDPDRKVGRLSVAEMQLVEIA
                     GAMRQRARVLVLDEPNSALSTRESERLFDLVRQLCGEGVTVIYVSHRLKEVLDLADRI
                     TVMRDGRVVETLDNHRLAEDRLIRAMVGRDLGAAKRWSLMADSRGAEVVLSAKNLTAA
                     SLDDISFDIRAGEILGIGGLPDSGKDNLGEALFGLRDRKGHVWVDGKPLLAGDPLAAI
                     RLGLSFAPADRRGAGGLLAMSVADNVVSASLPRFSIAGILRRGAIKREARAQVARLDA
                     RISRLGQKLGSLSGGNQQKIILGRGLVAHPRVLILHEPTRGIDVGAKAEIYEILRGIA
                     GEGVAVLLISSEIPELIMNAERVLVLRNGRISGELTRFEINEEAILVRAMAS"
     gene            5433..6383
                     /locus_tag="VKU03_03285"
     CDS             5433..6383
                     /locus_tag="VKU03_03285"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HLJ70314.1"
                     /translation="MTEPTITVVGSFAVGLTIRASKLPIFGETMLGSDFDMGPGGKGS
                     NQAVATARLGAKSALLAILGTDKLAGIATDLYAAEGVDASLVTGRSERGTGVGFIILN
                     HKGENFIILDMGANELMNAEAVDAGERRISKSDVVMTVLEVPTEAAARAMELGRKHGV
                     KTVLNPAPARQLPDSIFASVDYLTPNESELRILLGLPPDDPRSSRDLAGALRRKGARN
                     VIVTLGRSGALILNDELEIVIPAVEVAVTDTTGAGDAFNAGFAVALAEGRDIVAAVRF
                     GVICGGLACTKLGVIPSLPDRATADALYAQALQTVWKDKT"
     gene            6380..6841
                     /gene="rbsD"
                     /locus_tag="VKU03_03290"
     CDS             6380..6841
                     /gene="rbsD"
                     /locus_tag="VKU03_03290"
                     /EC_number="5.4.99.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006337043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016872 - intramolecular lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-ribose pyranase"
                     /protein_id="HLJ70315.1"
                     /translation="MNRNKLLNAELAHAIASMGHGDLMIVCDAGFPIPSSAWRIDLSI
                     VPDVPDLETVLTPIAQSFIAERVSYADTLPVHNAPLLAKIKRLFADADHEPIKHETIL
                     SEMAGRAKVIVRTGAFDPWGNILLYSGVDVSAWFSKAGVVAPDYYAKKMKR"
     gene            complement(6899..7417)
                     /locus_tag="VKU03_03295"
     CDS             complement(6899..7417)
                     /locus_tag="VKU03_03295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70316.1"
                     /translation="MPTDILTDRHLLLFGTIIQWFARYERLMEEVMANLAGADAAAVA
                     LMTRRLCFGDKRRAFLNMLRYRKVPIDQYDRVCEYLRVPRGLSALRDNIVHSTWGASR
                     APNLVQPDWVLNLPRSVQPPLGGVDAELIEGAEEKIGYAVEDLTEAAHSLALNYAALA
                     NYVKEIRPTAPR"
     gene            7620..7850
                     /locus_tag="VKU03_03300"
     CDS             7620..7850
                     /locus_tag="VKU03_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70317.1"
                     /translation="MTRDVATEPLETITIEPETRGDSRSLFRVFFNHRLLADQLTAAE
                     AHGLIGDLLERAALPNRAATIPLESLNASNDE"
     gene            8075..8317
                     /locus_tag="VKU03_03305"
     CDS             8075..8317
                     /locus_tag="VKU03_03305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70318.1"
                     /translation="MRLMVQRVGFVAALSFLLGGCLDAVTHPLNPEPTAYEDSLCPHG
                     PPGGEIVNYGCVAGRAVAPAAYHRHRRHRRIVRARY"
     gene            complement(8336..8758)
                     /locus_tag="VKU03_03310"
     CDS             complement(8336..8758)
                     /locus_tag="VKU03_03310"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HLJ70319.1"
                     /translation="MQDEEARIREIAHLLWETSGRPSGMAERHWRQAREIFEAEKAAL
                     VKQAPSFPVSSPPRQTMETHMRNFDELVADAQKALAHSLREAFDAGRAHTASELKHRM
                     AALFEDLISGPVAPHGAPAGHEGSGHEGQSEHSEPTSG"
     gene            8880..9122
                     /locus_tag="VKU03_03315"
     CDS             8880..9122
                     /locus_tag="VKU03_03315"
                     /inference="COORDINATES: protein motif:HMM:NF014577.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HLJ70320.1"
                     /translation="MRLQYLAMRLAAGMAIVFAAETGAAAAELPTMRSPPPKHAKPCR
                     VDGMEGYYLAGSNVCVRVSGYVSAGVAAGKSWSSSK"
     gene            complement(9136..10587)
                     /locus_tag="VKU03_03320"
     CDS             complement(9136..10587)
                     /locus_tag="VKU03_03320"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLJ70321.1"
                     /translation="MDGSFLIGVDVGTTSVKAALFDMAGRAQKTYSSAYATARPAPGW
                     AEQDPADWLSRVLAAIGELTAAAPAGAIAGLGLCSQVNTHVFVDEKGEALLPAILWQD
                     GRCAEDARQIDERVAIENRLQWWGAPLPIDASHVLSRMAFVRRAHPEIWRKTRWVMAP
                     KDYCLLRLAGEAAADPMSSFGVVDQNLAYIPELVALVPGAAERLPPLKGIAAPVGRVR
                     GGMPGAGLLVVNSAMDAWAGMLGAGAVRNGDAAYLSGTSEVGAIVSPRRIPTPGIIAF
                     PACEGIALHAGPTQAGGASVAWLAAMLGRSADEISALAALSDPARPAPIFLPHLQGER
                     APLWDIAARASFSGLDAAMGAPEMARAVLEGVAYSVRLLLGALEASSGVKTTRLHHAG
                     GGARSDLWCQIRADILGRAIERKAGLDSGVIGAAILAGVGAGIFASLAEGAGALVRPE
                     RVFEPAAARRARYDEGFARYQDLYRRLKGFSAG"
     gene            10691..>10900
                     /locus_tag="VKU03_03325"
     CDS             10691..>10900
                     /locus_tag="VKU03_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70322.1"
                     /translation="MPRIASRFVAALAGGVALLAASQALAGAKIAVITPYLAQPGTQF
                     YVEAFQDQAKANGWDVNVIDTKGDVA"
BASE COUNT         1948 a         3475 c         3562 g         1915 t
ORIGIN      
        1 acggcgcgct taaattcccg gctgaaatga tagacgtcgc agaagccgca agtctcggcg
       61 atttcggcga gcgccgccgg gctctgggta atcagagatt gggcaaggat gagacgctcg
      121 cgctgcagcc attggcgcgg actggcgcgt aaatatttgt gaaacaatcg cctgatctgc
      181 gaaggactca gctcggtcag cgcctcgatt tcgcgagcgc tccatcggcg gcgcaggttc
      241 cggcgcatcg cgttgacgat tttcgtcagc gcctgtggcg cgcccgcagc gtccgacgcc
      301 gacaaggcgc ggtcgacgat gagtaaaaat tcgccgacga gatggttgag gcgcagatca
      361 aggccaagtt cgcgccggcg catcgcgacg aacaaacgat cgaaccagga cgaaagattg
      421 gcgctttcgg gcatcgcgac tcgcggcggc ccatcgccga acagtttctg gcgcaacgcc
      481 gccgggttcg gcccatcgag ccgcagccag aggagtgtcc atggcgcgcg cgggtcggcg
      541 cgatgggcgt ggggtccttc attggcgatc caggcgagct ggcctgactg catatccagc
      601 ctttggccca gcgtttcgac gattccggcg cccgaaatac agtagaggat gtcctgcccg
      661 gtgtggctcg cgcgttcgat tccgtagtcg ggtcccgccg ccactttccc cgcccgcaac
      721 acgctaaatg cgctgagcgt ccatcccgcc tcgggaacgt agtgcaggct ctcgatgaac
      781 cgcgttccgg cagccatgcg cgttttatac aaaagaaggc cccttctgtc catttcgccg
      841 caggggccgc cgccgtagcc tgatcatagc gaatgcatga atcgactgga tcgcgcccga
      901 cgccgaaccg cccggacgcg caatccggga aaaggaggaa atcggacgat gacgctcgca
      961 atgacccagg aggaatcggc ccgctacgcg cgcgacggct acatcctgcg ccggggcctg
     1021 cttggcggcg acgaagtcgc caaattccgc aatcacgcgc gcgcgcaact cgagaaggag
     1081 caacgcgccg gcgcggtgat gacgaagggc gacaaagagg gaaaggccac ccttctcaaa
     1141 atgtggacca aggctgagga cgatcagtac ggccttctcg cccgcgacga gcgcatggtc
     1201 gatctggcgc aggacgcgat cggcaagccg atctacctct atagccataa gatgaccatg
     1261 aagcagccgc atgagggcgg cgcctgggaa tggcatcagg atttcggtta ctggtacaac
     1321 tatggctgcc tcgcgccgga tatgatgagc atctatgtcg ccttggacaa ggcgacgcgc
     1381 gagaacggct gtctgcaggt gctcaagggg tcgcacaagc tcggccgctt gaaccacatc
     1441 cgcgagaacg atcagaccaa tgtcgagcag gagcatcttg aagccgcgtt gaagcgcttc
     1501 gaactcgttt atgtcgagat ggacgcgggc gacgtgctgg tctttgacgg gaatctcttg
     1561 catcgctccg acgcgaaccg aaccgacacg taccgctggg gatatatctg ctcgtataac
     1621 gcggttgaga atgcgccgtt caagcgggtg cgcgaatacg gaaattacga ggaattgcgc
     1681 aaggtgccgg ctggctcgtt tcgcatggcg tcctgatctc attgcgcctg ctaaatctgc
     1741 gcgggcgcaa ccattcggcg ctcgcgaagg gacattcggc gaattcaata ggggaggtca
     1801 tgacgccggc gcagcgtccg cctcgccagg aggagaaaat ggcggatacg gcccggaccg
     1861 gcgcgcgcgc gccagccatg gaccgattta cgccgtcgcg cctgctcagg gcgcgcgagg
     1921 cgggcgtatt ttgcgcgctt ctcgcgctat gcctgttcct gaccttcgcg acctcgggct
     1981 ttttgacctc gatcaatctt ttcaatgtcg gccgacagat ttccctgctt gggatcatgt
     2041 ccgtcggcat gacattcgtg ctgatttcag gcgaagtcga tctctcggtc ggctcgacct
     2101 acgccttcgc cggccttacg accggcatgc tcatcatcgc caaatggtcg ctggcgccgg
     2161 ctattggcgc cggcgtcctg gcgggagccg ccatcggcct catcaatggg ctcctctcga
     2221 cctatggccg cttgccgtcg ttgatcgcga cgctcggcat gctcagcatg gcgcgcggcg
     2281 cggcgctcat tatgacgaac ggcgagccgg tgactgtcaa tgtccgtaac ggcgccgcgc
     2341 cggaagttct tgcgaaattc gagttcatgg gccagggcta ccttttcggc gtcgtgccca
     2401 tgcaactcgt cttcttcgcc gccgtcgcgg cgatcgcctg ggcggtgttg tcctatacga
     2461 atttcggctt ccggatattc gccgttggcg gaagcgcgaa ggccgctcgg gtctcgggaa
     2521 tcaatgtcag ccgtgtgaaa atctcggcct ttatcctgat gggcgggctg gcggctttcg
     2581 ggggaatcct gagcgtcgcc tttctgccca gcgcccaggc cggtcgaacc gggcttggcc
     2641 tcgaactcga cgtcatcgca gcgacgattg tcggcggcgc ctcgctttca ggcggcgagg
     2701 ggacgatcct cggcacaatc cttggcgtgc tgatcatcgg ggtcatgcgc aacggcttgg
     2761 tgctgatggg cgtctcgcca ttcgtacaag aattcctgat cggccttgtg atcgtcgtcg
     2821 cggtcggcat cgacaaatgg acgacccgtc gtgctgcctg acgaaatatt cagagggagg
     2881 agaaaagtga tgaaactgaa acatgtcttt ctcgcttttg cgatcgtttg cgcgccgttt
     2941 gcggctcagg cggcgcaaaa gctcagcgat tttccgctgg cggaccggat caaggcgaag
     3001 atcgcttccg ggaagccgct cgacatctat gtttcctatc acgatgtctc aaacgaattc
     3061 gcgccgtcca tcaagaaggg cgtgcagaag gcggccgccg acgacaaggt ggacgctcaa
     3121 ttcatcgggc cgacaggcgc caatgccgac gctcagatca gcgagatcga gacgctgatg
     3181 ggcaagatgg acggcttggc gatttcgtcg gtttcgagcg acgccttggc gccggtgatt
     3241 gaccgcgtgc tgaaggccgg catcccggtc gtgacgttca acaccgataa tccgaacagc
     3301 aagcgcttgg cttttgccgg gcaggatctg gttcaatcgg gtcgcgaggc gggcaagctg
     3361 atggtcaaag tgctcgacgg caaaggcaag gtcctgatca cgacgctgga cgccgccgcg
     3421 caatggtcgc tcgatcggga gaagggcgcg cgggaatcgc tcaaggaagc gcccggcatt
     3481 gaggtggtca atacgctgaa taccgggacc gacccgcaaa agatttattc ggcgatcgag
     3541 aacgccatgc tcgccaatcc ctcgatcacc ggcattctct cgctcgaatg ctgctcgact
     3601 ccggccgccg gaacctgggt tcagcgcaac aaagccatgg gaaaggtgaa ggtggtcggt
     3661 ttcgatctgc tcgatcagac ggtgcagctc gtccaggagg gagaaatcca ggcgacgatc
     3721 gaccaggccc cggaaaagca gggctttgaa gccgtcgacc tgctcgtcaa cttcctcaaa
     3781 ggaaaaccga tcgacaatgt cgacaccggc gtgaaaatct acaccaagga gaatatcgcc
     3841 gaagtcgcca agaaataaaa acgcgcgcgg cgacggcctg cctcgcgccg gcctcgtcgc
     3901 ggcttcgccg tcatgtctcg ccgcaacgaa cccgatctcg tcgaaatcgc cggggtgacc
     3961 aaaaggtttc ccggcgtgct cgcgcttgac cgcgtctcga tcagctttcg cgccggcgaa
     4021 gtgcatgcgg tcatcggcga gaacggcgcc ggcaagtcga cgctgatgaa cattctggcc
     4081 ggcgatctgc agccgaacga cggcgaaatc cgcatcgacg gccggccggt tcgcttttcg
     4141 tcgccgctcg ccagccgcgc cgcgggcatc gtcgtcgtct accaagagct tgcgctctgc
     4201 ccgactctga gcgtcgccga aaacatcatg atgtccgaag tggcgtcgca gccggtcgtc
     4261 gcgttcacgc cgcgcgccga gatgcgacgc caagcgcgca tggcgctgtc gcggctcggc
     4321 atggcttcgc tcgatcccga ccgaaaggtc gggcgcctga gcgtcgccga gatgcagctt
     4381 gtcgaaattg ccggcgcgat gcgccagcgc gcccgcgtgc tggtgctcga cgagcccaac
     4441 tccgcgctct cgactcgcga gagtgaaagg ctgttcgatc tcgtcaggca gttgtgcggc
     4501 gaaggcgtga cggtcatcta cgtctcgcac cgcctcaagg aggtcctcga cctcgccgac
     4561 cgcatcacgg tcatgcggga cggccgcgtc gtggagacgc tggacaatca tcgcctcgcc
     4621 gaagaccgtc tcatccgcgc catggtcggg cgcgatctcg gcgcggccaa acgctggtcg
     4681 ctcatggcgg attccagggg cgcggaggtc gtcctttcgg cgaagaatct gaccgccgcg
     4741 agcctggacg acatctcttt cgatattcgc gccggcgaga ttctcggaat cggcggtttg
     4801 ccggattccg gcaaagacaa tctcggcgaa gctctattcg gcctccgcga tcgcaagggg
     4861 catgtctggg tcgacggcaa gccgttgctc gccggcgatc cgctcgccgc gatccgttta
     4921 ggactctcct tcgctccggc cgatcggcgc ggggccggcg gcctcctggc gatgagcgtc
     4981 gccgacaatg tcgtctccgc gtccctcccc cgtttctcga ttgccggaat tctccggcgg
     5041 ggcgcgatca agcgcgaggc gcgcgcgcag gtcgcgcggc tcgatgcgcg aatttcgcgg
     5101 cttggccaaa aactcggttc gctctccggc ggcaatcagc agaagatcat tttgggacgc
     5161 ggccttgtcg cccatccgcg cgttctcatt ctgcacgagc cgacgcgcgg catcgatgtc
     5221 ggcgcgaagg ccgagattta cgaaatcttg cgcggcattg ctggcgaggg cgtcgcggtc
     5281 ctgttgatct cgtcggaaat tccggaattg atcatgaacg ccgagcgcgt cctcgttttg
     5341 cgcaatggcc gcatatccgg cgagttgacg cggttcgaga tcaacgagga agccatcctc
     5401 gttcgggcga tggcgtcttg acggggcgag cgatgacgga gccgacaatc acggtggtcg
     5461 gaagcttcgc ggtcggcttg acgatccgcg cgtcgaaatt gccgatcttc ggcgaaacca
     5521 tgctgggctc ggatttcgac atgggacccg ggggcaaggg ctccaaccag gcggtggcga
     5581 cggcgaggct cggcgcgaag tccgcgcttc tggccatcct tggtacggac aagcttgccg
     5641 gcatcgccac cgatctctat gccgccgaag gggtggacgc ctcgctggtg acgggccgaa
     5701 gcgagcgcgg gaccggggtc ggcttcatca tcctcaatca taagggcgag aatttcatca
     5761 tcctcgacat gggcgccaac gagttgatga acgccgaggc ggtggacgcc ggcgagcggc
     5821 gcatttccaa gagcgacgtg gtgatgaccg tgctcgaagt gccgaccgaa gcggcggcgc
     5881 gagcaatgga gctcggccgt aagcatggcg tcaagacggt cctgaacccg gcgccggctc
     5941 gacaactacc cgattcgatt ttcgcctcgg tggactatct gacgccgaac gagagcgagc
     6001 tgcgcatcct gcttggccta ccgccggatg atccgcgctc gtcgcgcgat ctcgccgggg
     6061 cgctgcgccg aaagggcgcg cgcaatgtga ttgtcacgct gggccgcagc ggcgctctga
     6121 ttctcaatga cgaactcgag atcgtcatcc ccgcagtcga agtcgcggtc accgacacga
     6181 ccggcgctgg cgacgcgttc aacgccggct tcgcggtcgc cttagccgaa ggccgcgaca
     6241 tcgttgccgc cgtgcgcttc ggcgtgatct gcggcggcct tgcctgtaca aagctcggcg
     6301 tgatcccgag cctccccgat cgcgcgaccg ccgacgcgct ctatgcgcaa gccctgcaaa
     6361 ccgtatggaa ggacaagacg tgaatcgcaa caagctgctc aacgccgagc tcgcgcacgc
     6421 catcgcttcg atgggccacg gcgatttgat gatcgtctgc gacgcgggtt ttccaatccc
     6481 atcgagcgca tggcgcatcg atctctcgat cgtcccggac gtgccggatc tcgagacggt
     6541 gctgacgccg atcgcgcaaa gcttcatcgc cgaacgcgtc tcctacgccg atacgctgcc
     6601 ggtccataac gcgcctctgc tcgccaagat caagcgtctc ttcgcggacg ccgaccatga
     6661 gccgatcaag catgagacca tcctctcgga gatggccgga agggcgaaag tcatagtacg
     6721 aaccggcgcc ttcgatccct ggggcaatat tctcctctat tcgggcgtcg acgtctcggc
     6781 ctggttctcc aaagccggcg tcgtcgcgcc ggactattac gccaagaaga tgaagcgctg
     6841 acgccgcgca tctttggcgc gtggacattt tcgcgcggac gcccgccgtt tcgcggctct
     6901 agcgcggcgc ggtcgggcgg atttctttca cgtaattcgc gagcgcggcg tagttcagcg
     6961 cgaggctgtg ggcggcttcg gtcaaatctt cgacggcgta gccgatcttc tcttcggcgc
     7021 cttcgatgag ctcggcgtca acgccgccga gcggcggctg gacgcttcgc ggcaggttga
     7081 gcacccaatc cggctgcacc aggttcggcg cgcggctggc gccccaggtc gagtggacga
     7141 tgttgtcgcg cagcgcgctg agaccgcgcg ggacccttaa atattcgcag acccggtcat
     7201 actgatcgat cgggactttc cggtagcgca gcatattgag aaaggctcgt cgcttgtcgc
     7261 cgaagcatag gcgtcgcgtc atcagcgcga ccgcggcggc atcggcgccg gcgagattgg
     7321 ccatgacctc ctccatcagc cgctcatagc gcgcaaacca ttggatgatg gttccgaaga
     7381 ggagcaaatg gcggtcggtg agaatatcgg tcggcatgtc cggtctccgc cgcatcgccg
     7441 cgcccgcgcg cgatccgcca tcctcgtggg agggaaggca tgaatcaact cgccgcgatc
     7501 aagaaagagc gccgctcgcc tgtccggttt cggagcgcct ggaggcgccg gcgatggcgg
     7561 cggtcgcaag cgctgcggca aaggcgggaa ccacaagggc gtcgacatgt tgttgaggga
     7621 tgacgcgcga tgtcgcaacc gagcctctcg agacgatcac catcgagccg gaaacgcgcg
     7681 gcgattcgcg aagcctgttt cgcgtcttct tcaaccatcg attgctggcc gaccaattga
     7741 cggccgccga ggcgcacggc ctgatcggcg acctcctcga gcgagcggcg ctgcccaacc
     7801 gcgcggcgac catcccgctc gaaagcctca atgcaagcaa cgacgaatga aatcaaaaag
     7861 atcggccccg ccgacgagga cggggccgac ccaacggcgc gcgggctggg gggcaaaaag
     7921 gcgcgccgca ctgacgatgg aaaccgctga cggttgggaa agttccgagc ctcgcgcgaa
     7981 aaagttgcgg acggagaatc cgagggccaa gatcgccccg ccctgagccg gaacggcgca
     8041 ttggccggcg tcaggtgatt tggggagccc tggcatgagg cttatggtgc agcgcgtcgg
     8101 ctttgtggcc gcgctttcgt tccttctcgg cggctgcttg gacgcggtca cccacccgct
     8161 caatcccgag ccgacggcct atgaagacag tctctgcccg cacggcccgc ccggcggcga
     8221 aatcgtcaat tacggctgcg tggcagggcg ggcggtcgcc ccggccgcct accatcgcca
     8281 ccgccgccac aggcggatcg tgcgcgcgcg ctattagccg tattgcgccc ggcgctcacc
     8341 cggatgtcgg ctccgaatgc tcgctttggc cttcgtggcc ggagccttca tggccggccg
     8401 gcgcgccatg cggcgccacc ggaccgctga tcagatcctc gaagagggcg gccattctgt
     8461 gcttcagttc cgaagccgtg tgagcgcgtc ccgcgtcgaa cgcttcccgc aaggagtgcg
     8521 cgagcgcttt ctgagcgtca gccaccaact cgtcgaaatt gcgcatgtga gtttccatcg
     8581 tttgtcgagg cggcgatgat acgggaaaac taggcgcttg cttgaccaaa gcagccttct
     8641 ccgcctcgaa aatttcgcgc gcctgccgcc agtggcgctc ggccatgcct gaagggcggc
     8701 cggacgtctc ccaaagcaga tgggcgattt cgcggatgcg tgcttcctcg tcctgcaagc
     8761 tgcgctttcc ttaaacgggc cgggcgcgcg gcggcgcttg cgcctggagc gcgccagaat
     8821 aaactaagtc ctacgccatt tcaggcgggt tcgccgggta tcgttagcgg aggtcagcga
     8881 tgcggcttca atacctggcg atgcggctcg cggccggcat ggctatcgtc ttcgccgccg
     8941 agaccggcgc ggcggctgct gaactgccga caatgaggtc gccgccgccg aagcacgcca
     9001 agccctgccg cgtcgacggc atggagggct actaccttgc cggcagcaat gtctgcgtca
     9061 gggtcagcgg ctatgtttcc gcgggcgtcg ccgccggaaa gagctggtcc tcctctaaat
     9121 aaaagccgga atcccctacc cggccgagaa ccccttgagc cgccgataga ggtcctgata
     9181 gcgcgcaaag ccctcgtcat agcgcgcgcg gcgcgcggcg gccggctcga aaacgcgctc
     9241 gggtcgaaca agagcccccg cgccctccgc cagcgaagcg aaaatgccgg cgcccacgcc
     9301 cgcgagaatg gcggcgccga tcacgccgga atcgagcccg gcttttcgtt cgatcgcgcg
     9361 gccgagaata tcggcgcgga tttggcacca gagatcggag cgcgcgccgc cgccggcgtg
     9421 atgcagcctt gtcgtcttga cgccggacga ggcttcgagc gcgccgagca gaagcctcac
     9481 cgaataggcg acgccttcga ggacggcgcg ggccatttcc ggcgcgccca tcgcggcgtc
     9541 gaggcccgaa aacgaggcgc gcgcggcaat gtcccaaagc ggcgcgcgct cgccttgcag
     9601 atgcggcagg aagatcggcg ccggccgcgc cgggtcgctc aacgcggcga gcgcggaaat
     9661 ctcgtcggcg ctccgcccca gcatcgcggc gagccaggcg accgaggcgc cgccggcctg
     9721 ggtcggcccg gcatgaagcg cgatcccctc gcaagccgga aaggcgatga ttccgggcgt
     9781 cgggatgcgg cgcggcgaga cgatcgcgcc gacttcgctc gtgccgctga gataggcggc
     9841 gtcgccgttc cggacggcgc cggcgccgag catgcccgcc caggcgtcca tcgcgctgtt
     9901 gacgacaagc aggccggcgc ccggcatgcc gccgcgcacc ctgccgaccg gcgcggcgat
     9961 cccctttaag ggcgggagcc gctcggccgc gccaggaacg agggcgacga gttcgggaat
    10021 ataggcgagg ttttggtcga caacgccgaa cgacgacatc ggatcggccg ccgcttcgcc
    10081 ggcgagccgg agaaggcaat aatccttcgg cgccatgacc cagcgcgttt tgcgccatat
    10141 ttcgggatgg gcgcgcctga cgaaagccat tcggctgaga acatggctgg cgtcgatcgg
    10201 cagcggcgcg ccccaccatt gcagccggtt ttcgatcgcc acccgctcgt cgatctggcg
    10261 cgcgtcctcg gcgcagcggc cgtcctgcca caggatcgcg ggcagcagcg cctcgccctt
    10321 ctcatcgacg aaaacatggg tgttgacctg cgagcaaagg cccagtccgg cgatcgcgcc
    10381 cgccggcgcc gcggcggtca attcgccgat agcggcgagg acgcgcgata gccagtcggc
    10441 cgggtcctgc tcggcccagc cgggcgccgg gcgcgccgtc gcataggcgc tggaataggt
    10501 cttttgcgcg cggccggcca tgtcgaacaa agcggccttg accgacgtgg tgccgacatc
    10561 gacgccgatg agaaaggagc cgtccatatg cgcaaaatcc ccctgcggcg cgactatagc
    10621 cggtcggttg cgctttgtgg tcgcgatgct agtttggctt gcgcggcgct tggatcgtta
    10681 ggggggataa atgcccagaa ttgcttctcg ttttgttgcg gcgctggccg gcggggtcgc
    10741 cctgctcgcc gcctcgcaag ccctggcggg cgccaagatc gcggtgatca cgccttatct
    10801 ggcgcagccc ggcacgcaat tctatgtcga agcgttccag gatcaggcca aggccaatgg
    10861 ctgggacgtc aacgtgatcg acaccaaggg cgacgtcgcc
//
LOCUS       DATGJW010000114         6241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_850358, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000114 DATGJW010000000
VERSION     DATGJW010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6241
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_850358"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..328
                     /locus_tag="VKU03_03330"
     CDS             <1..328
                     /locus_tag="VKU03_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70323.1"
                     /translation="LMDPDQNGVFIAEDKTGAKDVKPRRYVYYFVRVLKSGERVDLYD
                     FTAADWRRLPPDLKRRLKPGASVSVADDADAAAILRAIERRLAERPTLRKTTFRLVRR
                     LDKAER"
     gene            417..1454
                     /gene="hemB"
                     /locus_tag="VKU03_03335"
     CDS             417..1454
                     /gene="hemB"
                     /locus_tag="VKU03_03335"
                     /EC_number="4.2.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011664423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004655 - porphobilinogen synthase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porphobilinogen synthase"
                     /protein_id="HLJ70324.1"
                     /translation="MPVHPLRPAPAAEPTAPRLNLARRPRRNRKAEWTRRLVAENVLT
                     AADLIWPIFLVESRAPRMPVAAMPGVDRVNVDEALRLAERAVALKIPAIAFFPYTEAA
                     LKDEAGSEAFNEQNLVCRACRAIKREFPELGVVTDVALDPYTSHGHDGLMAGDKILND
                     ETVAALTRQALNQARAGADVIAPSDMMDGRVGAIRSALDAEGFLDVQIMAYAAKYASA
                     FYGPFRDAIGSKGALKGDKRTYQMDPANSDEALREVAADLDEGADMVMVKPGLPYLDI
                     VRRVKDEFGAPTFAYQVSGEYSMIMAAAQNGWLDGDKAMMESLIAFKRAGADGVLTYF
                     APRAAEALKGR"
     gene            1564..1785
                     /locus_tag="VKU03_03340"
     CDS             1564..1785
                     /locus_tag="VKU03_03340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606483.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70325.1"
                     /translation="MTKLLDQAVETARRLGPDQQDQIARMMLFYAETPVIQLTPEEDA
                     DLAAAEEEVARGELATDEQMRAIWAKHQL"
     gene            1885..3222
                     /locus_tag="VKU03_03345"
     CDS             1885..3222
                     /locus_tag="VKU03_03345"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70326.1"
                     /translation="MPRLASILTAFAFLALLASCAPLLEADQARLCRMALPAIEPPDA
                     TIVVKRQTPFADRKGVRIDYRSNQPGEEPAAHYAECRFREPGRPRRSQDLVAVDTDRG
                     PLSAYQVSILIRFWLSTPEARADDPEPLAGAQQAWRGPRPLIYALQQAINGLPLAAIY
                     ALLAAAYSLVYGLVGRINLAFGELAAAGGYAATIGVLLAAAFAPWGPIALGLCLAVAT
                     AIAFGVASGKLVYAPLHRATGQQVMVAAVGLALFLQEFLRLTQGGRLRWINPILNEPF
                     AVARSGDFVAAATPIALIVGAVALIAAAGLLLAMKATRFGRQWRAYADDPLAAEMFGV
                     DSKAIFAKTFALASAMAGLSGFVMTVFYGSVGYGASTALGLKALIAAILGGIGSVPGA
                     FLGGLIIGGFEALWSAVFPIDYRDVAVYSLLAIVLILRPGGIMNLSAYTAPRT"
     gene            3318..3767
                     /locus_tag="VKU03_03350"
     CDS             3318..3767
                     /locus_tag="VKU03_03350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RDD family protein"
                     /protein_id="HLJ70327.1"
                     /translation="MSLIDAHIVSGPTLRAESFDGVRRRRAIALCLDLVLVSILAAVI
                     WCALAILTFGLSIVLLPPIFPLVAFFYNGLTISGWRMATPGMRAMDLEMRMQDTGGRV
                     PFINAAAQALFFYLSWMLPAVFLVTLVDREKRFLHDILAQVVVIRRL"
     gene            3881..4633
                     /locus_tag="VKU03_03355"
     CDS             3881..4633
                     /locus_tag="VKU03_03355"
                     /EC_number="2.3.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012456455.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004057 - arginyl-tRNA--protein
                     transferase activity [Evidence IEA];
                     GO_process: GO:0016598 - protein arginylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginyltransferase"
                     /protein_id="HLJ70328.1"
                     /translation="MSREPRDAPQFYLTAPSPCPYLPGVEERKVFTHLVGRRAAALND
                     MLTQTGFRRSQTIAYRPACEQCRACVSVRVLVEDFAPTRNMRRVAEVNADLIGYMGPP
                     KPTAEQYSLFRAYLETRHPDGGMADMSVLDYSMMIEDSHIDTHVIEYRRRGPDHFITK
                     RGEGKLYAACLTDKLADGLSMVYSFFDPDEGRRSLGSYMILDHIEKARKLGLPHVYLG
                     YWVEGSKKMAYKGRYLPQERLGMDGWRRVERG"
     gene            complement(4816..5934)
                     /gene="ald"
                     /locus_tag="VKU03_03360"
     CDS             complement(4816..5934)
                     /gene="ald"
                     /locus_tag="VKU03_03360"
                     /EC_number="1.4.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012110464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000286 - alanine dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006522 - alanine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HLJ70329.1"
                     /translation="MLVGVPKEIKDNEFRVGLTPSSVAELVHHGHQVWVEKSAGFGSG
                     LPDSEYAQAGANLVDGPDEIFARAEMIVKVKEPLAAEREKLRRGQVLFTYLHLAPDLE
                     QTKDLIASGATCIAYETVTSPHGGLPLLTPMSEVAGRLAPQVGAHALEKAAGGRGILL
                     GGVPGVPAAEVIILGGGVSGTHAATIALGMGANVTVVDRSAEALRRLSAQFGTGVRTI
                     FSTRTAIEELVKRADLLIGTVLVPGAAAPKLVTRAMLKTMKPGAVIVDVAIDQGGCCE
                     TSKPTTHSHPTYVVDDIVHYCVANMPGAVARTSTFALNNATLPFALALADKGWRQALK
                     DDAHLRAGLNVHDGKVTCKPVAEAHGLAYAPAETILGA"
     gene            6080..>6241
                     /locus_tag="VKU03_03365"
     CDS             6080..>6241
                     /locus_tag="VKU03_03365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix transcriptional
                     regulator"
                     /protein_id="HLJ70330.1"
                     /translation="MLEALDHRDRAILRALMRDGRLANADLAERVNLSPSACLRRVRM
                     LEESGLIAGY"
BASE COUNT          987 a         2164 c         1997 g         1093 t
ORIGIN      
        1 cttgatggat cccgaccaga acggcgtctt catcgccgag gacaaaaccg gcgcgaagga
       61 cgtgaagccg cgtcgttatg tctattattt cgttcgggtt ctgaagtcgg gcgaacgggt
      121 cgatctctat gatttcacag cggcggattg gcggcgcttg ccgcccgacc tcaagcgccg
      181 cttgaagccc ggggcgagcg tcagcgtcgc cgacgacgcc gacgccgccg cgatcctgcg
      241 agcgatcgag cgaaggctgg cggagcgccc gaccctgcgg aagacgacgt tccggctggt
      301 gcgacggctc gacaaagccg agcgctgacc ccggcattgc tgcgacatcg ccgcctgtcg
      361 cctcgccgcc cgccatgata gaaatccgcc ccgcattctc gcgccgagcc cccgccatgc
      421 ccgtccatcc gctccgccct gcccccgccg ccgaaccgac cgcgccgcgt ctcaacctgg
      481 ctcgccgtcc gcgccgcaac cgcaaggcgg aatggacgcg ccggcttgtc gcggaaaacg
      541 tcctgaccgc agccgatctg atctggccga tctttctcgt cgagagccgc gcgccgcgca
      601 tgcccgtcgc cgccatgccg ggcgtcgacc gggtcaatgt cgacgaagct ttgcgtctgg
      661 ccgagcgcgc cgtcgcgctg aaaatcccgg cgatcgcctt ctttccctat accgaggcgg
      721 cgctgaagga cgaggcgggc agcgaagcct tcaacgagca gaatctcgtc tgccgcgcct
      781 gtcgggcgat caagcgcgaa tttcccgagc tcggcgtcgt taccgatgtc gcgctcgatc
      841 cctatacgag ccacggccac gacggcttga tggcgggcga caaaatcctc aacgacgaga
      901 cggtcgcggc gctgactcgc caggcgctca accaggcgcg ggccggcgcc gacgtgatcg
      961 cgccgtccga catgatggac ggccgggtcg gcgcgatccg ctcggcgctc gacgcggaag
     1021 gctttctcga cgtgcagatc atggcctacg ccgccaaata cgcgtcggcc ttttacggcc
     1081 cgttccgcga cgcgatcggc tccaagggcg cgctcaaggg cgacaagcgc acctaccaga
     1141 tggacccggc caacagcgac gaagccctgc gcgaagtcgc cgccgatctc gacgaaggcg
     1201 ccgacatggt catggtcaag cccggcctgc cctatctcga catcgtccgg cgggtgaagg
     1261 acgaattcgg cgcgccgacc ttcgcctacc aggtctccgg ggaatattcg atgatcatgg
     1321 ccgcggcgca gaacggctgg ctcgacggcg acaaagcgat gatggagagc ctgatcgcct
     1381 tcaagcgcgc gggggcggac ggagtgctga cgtactttgc gccgcgggcg gcggaggcgc
     1441 tgaaggggcg gtgagcggcg ctgcggttcg gtgcagctaa ggaccgagcg tccccggatt
     1501 aaggctcctt atttcttcgc aactctatgc ataatggcgg ctttacgagg cggcgaggac
     1561 accatgacca aattgctcga ccaggcggtt gagaccgcgc ggcggctggg gcctgaccag
     1621 caggatcaaa tcgcccgcat gatgctgttc tacgccgaaa cgcctgtcat ccaactcacg
     1681 ccggaggaag acgctgacct tgccgccgcc gaggaagaag tcgcacgcgg cgaattggcg
     1741 acggacgaac agatgcgcgc catctgggcg aagcatcagc tttgaagctg cgctacagcg
     1801 accgcgcagc gccgcgatgc ggcgaataac cggctaaatc gcggacacta gatttagccc
     1861 gcgccctgcc ccattgtggc gaacgtgcct cgtctcgcgt cgattctcac cgctttcgct
     1921 tttctcgcgc tgctcgcgag ctgcgcgccg cttttggagg ccgatcaagc gcggctgtgt
     1981 cgcatggctt tgccggcgat cgagccgccg gacgcgacca tcgtcgtcaa gcgccagacg
     2041 cccttcgccg accgtaaggg cgtgcgcatc gactatcgat cgaaccagcc cggcgaagag
     2101 cccgccgccc attacgccga atgccgtttc cgcgagccgg gccggccgcg ccgctcgcag
     2161 gatttggttg cggtcgacac cgatcgcggg ccgctcagcg cctaccaagt gtcgatcctg
     2221 atccgtttct ggctatcgac gcccgaggcg cgggccgacg atcccgagcc gctcgccggc
     2281 gcgcagcagg cctggcgcgg gccgaggccg ctcatctacg ccctgcagca ggcgatcaac
     2341 ggcttgccgc ttgcggcgat ctacgccctt ctcgccgccg cctattcgct ggtctacggc
     2401 ctcgtcgggc ggatcaacct cgccttcggc gaactcgcgg cggcgggcgg ctacgccgcg
     2461 acgatcgggg tcctgctcgc cgccgctttc gccccttggg ggccgatcgc gcttggcctc
     2521 tgcctggccg tcgccacggc gatcgccttc ggcgtcgctt cgggcaagct cgtctatgcg
     2581 ccgctccacc gcgccaccgg ccagcaggtc atggtcgcgg cggtcggctt ggcgctgttc
     2641 ctgcaagaat tcctgcgcct gacccagggc gggcgcctgc gctggatcaa tccgatcctc
     2701 aacgagccct tcgcggtggc gagatcgggc gatttcgtcg ccgccgcgac gccgatcgcc
     2761 ttgatcgtcg gcgcggtcgc cctgatcgcc gccgccggcc tgctcctggc gatgaaggcg
     2821 acccgcttcg gccggcaatg gcgcgcctat gccgacgatc cgctcgccgc ggaaatgttc
     2881 ggcgtcgact ccaaggcgat tttcgccaag actttcgccc tggccagcgc catggccggg
     2941 ctttccggct tcgtcatgac cgttttctat ggctcggtcg gctacggggc ttcgaccgcg
     3001 ctcggcctga aagccctgat cgccgccatt ctcggcggca tcggctcggt gcccggcgcc
     3061 tttctcggcg gcctgatcat cggcggcttc gaggcgctgt ggtcggcggt gttcccgatc
     3121 gattaccgcg acgtggcggt ctatagcctg ctcgccatcg tcctcatcct aaggcccggc
     3181 gggatcatga atttgagcgc ctatacggcg cccaggacct gatgtcattc ctgggaatga
     3241 caagaccccg taggccttga tcgcctgtcg cgcctcccca cattacgcgc ggccaaaccg
     3301 cgccgcagga gcgctccatg tctttgatcg acgcccatat cgtttccggc ccgacgcttc
     3361 gcgccgagtc cttcgacggc gtgcgccggc ggcgggcgat cgcgctttgt ctcgatctcg
     3421 tcctcgtctc gatcctcgcc gccgtgatct ggtgcgcgct ggcgatcctg accttcggcc
     3481 tgtccatcgt cctcttgccg ccgatcttcc cgctcgtcgc gtttttctac aacgggctga
     3541 cgatctcggg ctggcgcatg gcgacgccgg gcatgcgggc gatggatctc gaaatgcgga
     3601 tgcaggatac gggcggccgc gttcccttca tcaacgccgc ggcgcaggcg ctgttcttct
     3661 acctgagctg gatgctgccg gcggtgttcc tggtgacgct ggtcgaccgc gagaagcgat
     3721 tcctccacga catcctggcc caggtcgtgg tcatccgccg cctctgatcg tcagaaagtg
     3781 aatgtgaacg gggcggcgaa atttcgccag gaccattccc gccgggccga atccgttcta
     3841 ccttccttaa ggcctgacgg cgtgagggag atgggaaggc gtgtcgagag aaccccgcga
     3901 cgccccgcaa ttctacctga ccgcgccctc gccctgccct tacctgcccg gggtcgagga
     3961 gcggaaagtc tttacccatc tggtcgggcg gcgcgctgcg gcgctgaacg acatgctgac
     4021 ccagaccggc ttccgccgct cgcagaccat cgcctaccgc ccggcctgcg agcaatgccg
     4081 cgcctgcgtc tcggtccgcg tcctggtcga agatttcgcg ccgacgagaa acatgcggcg
     4141 cgtcgccgag gtcaacgccg acctgatcgg ctatatgggc ccgcccaagc cgaccgccga
     4201 gcaatattcg ctgttccgcg cctatctcga aacccgccat ccggacggcg gcatggccga
     4261 catgtcggtg ctcgattact cgatgatgat cgaggacagc catatcgata cgcatgtgat
     4321 cgaatatcgc cgccgcggac cggaccattt catcaccaag cgcggcgaag gcaagctcta
     4381 cgccgcctgc ctgaccgaca agctcgccga cggcctgtcg atggtctatt ccttcttcga
     4441 tcccgacgag ggccgccgct cgctcggctc ctacatgatc ctcgatcaca tcgaaaaggc
     4501 gcgcaagctc ggcctgccgc atgtctatct cggctactgg gtcgagggct cgaagaagat
     4561 ggcctataag ggccgctacc tgccgcagga gcggctgggc atggacggct ggcggagagt
     4621 ggagcggggg tagtcccctc gtcccgcgca gcggggagag ggtggccgcg aagcggccgg
     4681 gtgaggggcg gcgccgaact tgcagaacgc gctctttcgt acctcgcgga ggtacacaaa
     4741 tccccaagtc ctgcgcagcc cctcatccgg cgcttcgcgc caccttctcc ccgcttcgcg
     4801 gggcgaaggg gaggctcatg cgccgagaat ggtttccgcc ggcgcatagg cgagcccatg
     4861 cgcctcggcg acgggcttgc atgtgacctt cccgtcatgg acgttgaggc cggcgcgcag
     4921 gtgagcgtcg tccttcagcg cctgccgcca gcctttgtcc gcgagcgcca gcgcaaaggg
     4981 cagcgtcgcg ttgttcagcg cgaaggtcga ggtgcgcgcg accgcgcccg gcatattggc
     5041 cacgcaataa tggacgatgt cgtcgacgac ataggtcggg tgcgaatggg tcgtcggctt
     5101 cgacgtctcg cagcagccgc cctggtcgat ggccacatcg acgatcaccg cgccgggctt
     5161 catcgtcttg agcatggcgc gggtgacgag cttcggcgcg gcggcgccag gcaccagcac
     5221 ggtcccaatc agcagatcgg cgcgcttgac cagttcctcg atcgcggtgc gtgtcgagaa
     5281 gatcgttcgt accccggtgc cgaattgcgc cgacagtcgg cgcagagctt cggccgagcg
     5341 gtcgacgacc gtgacattcg cgcccatgcc gagcgcgatc gtcgcggcat gcgtgccgga
     5401 gacgccgccg ccgagaatga tcacctcggc cgccggcacg cccggcacgc cgccgagcag
     5461 gatgccgcgg ccgcccgccg ccttttcgag ggcatgggcg ccgacttgcg gcgccaggcg
     5521 cccggcgact tccgacatcg gcgtcagcag cggcagcccg ccatgcggcg aggtgacggt
     5581 ctcataggcg atgcaggtcg cgccggaggc gatcaggtcc ttggtctgtt ccagatcggg
     5641 cgcaagatga aggtacgtga acaagacttg cccgcgccgc agcttctcgc gttccgccgc
     5701 gagcggctcc ttgaccttca cgatcatctc ggcgcgagcg aatatctcgt ccggcccgtc
     5761 gaccagattg gcgccggcct gcgcatattc cgaatcggga aggcccgaac cgaatccggc
     5821 cgatttttcg acccaaacct gatggccgtg atggacgagc tcggccactg acgacggcgt
     5881 caggccgacc cgaaattcat tgtccttgat ctccttcggc acgccgacca gcatgacgac
     5941 cccctctgca agaaagccgc cggcatgatc cgccatttgc cgcgcaattt cccgcgatag
     6001 tggcggctgt cgtgaagcgt tttcgaatct tattcgatga tttttgtatt gatcgcaatt
     6061 tcatgcgaag ctggcaggca tgctcgaagc gctcgatcac cgcgaccgcg cgattctgcg
     6121 cgcgctgatg cgcgacggcc ggctcgccaa cgccgatctc gccgagcgcg tcaacctctc
     6181 gccttcggcc tgcttgcgcc gcgtccgcat gctcgaagag agcggcctca tcgccggcta
     6241 t
//
LOCUS       DATGJW010000115         2719 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_642929, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000115 DATGJW010000000
VERSION     DATGJW010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2719)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2719)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2719
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_642929"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..992)
                     /locus_tag="VKU03_03370"
     CDS             complement(<1..992)
                     /locus_tag="VKU03_03370"
                     /EC_number="2.7.7.13"
                     /EC_number="5.3.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004476 - mannose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate
                     guanylyltransferase/mannose-6-phosphate isomerase"
                     /protein_id="HLJ70331.1"
                     /translation="MPKIMPVIMCGGSGTRVWPESRESLPKQFIPLIGQRSTFQSIVS
                     LVSDPEVFDRPVVITNFAYRFRVSEQLREIGAEAAILLEPDRRDSAAAVGAAAAWAHA
                     RDAATVVAMLAADHVFEDGKAFARLCAQAGAAAAAGEIVAFGVTPDYPATGYGYIHPG
                     PPLARDPRLRRIERFVEKPDETRARAFIDEGYLWNSGNFVFRADVMLEELERFEPQIA
                     SATRAAVNAARKDLDFIVLDHESFAKAPKISIDYAVMERTDKAVVLTADVGWSDVGEW
                     SAVWRLSPRDANGNSLRGRVAALDSSNVLARSEEQLVAVIGLKDVVVVATGDAVL"
     gene            complement(1096..2280)
                     /locus_tag="VKU03_03375"
     CDS             complement(1096..2280)
                     /locus_tag="VKU03_03375"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70332.1"
                     /translation="MNPAVQTPGGAKVGAALHVAVAAMFFALGVGMGLWAGASGAILV
                     HAGIDPATFGVLLTVYIGAYLVAMSAGGTLAQRFGITQALSVSAIVYGATLCALLDAS
                     SKTWVAIALVISGFGGVVDVLMNAEGARIERRLGRPIMARLHAAASAGIALGAILGSV
                     IFAGPAPWAAGVLAALTLAGAGVAYHRAARSDPPPPSPAGTNRRSGLSFAPALIGLGI
                     VVGASTAAELAAQLWSTLFLREEAPRLAAISGLGAAFFAACQAVLRVNVDAIRLRVRD
                     LRIIMASFAIAALGFALVSAHAGFAASVAGFALIGVGTGPIVPCGFALAARQSAAGPA
                     VGLASASLFSAVTRLPAPMATGAIAQAFSLPVAFGAFALALAVTAAAVAVAASVRARR
                     EA"
     gene            complement(2277..2711)
                     /locus_tag="VKU03_03380"
     CDS             complement(2277..2711)
                     /locus_tag="VKU03_03380"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HLJ70333.1"
                     /translation="MGGVNFLMHGAGWMEGGLQASFEKMALDADLLAMVAEFLKPMRV
                     DESELALDAMREVGPGGHFFGAAHTQARYRTAFFSPMISDWRNYETWREAGSPTALDA
                     AERIVVGRLRDFVAPELEPERLEALGAFVARRKAEGGAPTDF"
BASE COUNT          470 a          946 c          954 g          349 t
ORIGIN      
        1 aggaccgcgt cgccggtggc gacgacgacc acgtccttaa gcccgatgac cgcgaccaat
       61 tgctcttccg aacgcgccag cacattcgag gaatcgagag ccgcgacccg cccgcgcagg
      121 ctattgccgt tggcgtcgcg cgggctcaac cgccagaccg ccgaccactc gccgacgtcc
      181 gaccagccga cgtcggcggt caggaccacg gccttgtcgg ttcgctccat taccgcatag
      241 tcgatcgaga ttttgggcgc cttcgcgaag gactcgtgat cgaggacgat gaagtcgagg
      301 tccttcctcg cagcgttgac cgccgcccgg gtcgcgctcg cgatctgagg ctcgaaacgc
      361 tcgagctcct ccagcatgac gtcggcgcga aagacgaaat tgcccgagtt ccagaggtag
      421 ccctcatcga tgaaggcgcg cgcgcgggtt tcgtcgggct tctccacgaa acgctcgatt
      481 cggcgcaagc gtgggtcgcg cgcgagcggg gggccggggt gaatatagcc gtagccggtc
      541 gctggatagt cgggggtgac gccgaaggcg acgatttcgc cggccgccgc cgccgcgccg
      601 gcttgagcgc aaagcctggc gaacgccttt ccgtcctcga acacgtggtc ggcggcgagc
      661 atggcgacga ccgtcgccgc atcgcgggcg tgagcccagg cggccgcggc gccgacggcg
      721 gcggcgctgt cgcgccggtc aggctcaagc aggatcgcag cctccgcgcc gatctcccgc
      781 aactgctcgc tcacccgaaa gcgataggcg aaattggtga tcaccaccgg cctgtcgaac
      841 acctcgggat cgctgaccag gctcacgatc gattgaaagg tcgaacgctg gccgatcagg
      901 ggaatgaatt gcttgggcag gctttcccgc gattccggcc acacccgggt ccccgacccg
      961 ccgcacatga tcaccggcat gattttgggc acgtgttccc tcgcgttgac tgccagcgtc
     1021 cgcaatcacc agccgcgcgc tgttccctgc aattgtggcc gcgccaagtc aatgctcggc
     1081 ctcatgtctc gagcttcacg cctcgcgccg cgccctcacg gaggcggcga cggcgaccgc
     1141 ggcggcagtt acggccaggg ccagcgcgaa ggcgccgaac gcgaccggca gcgagaaagc
     1201 ctgcgcgatc gccccggtgg ccatcggagc gggcaggcgg gtcaccgcgc tgaacagcga
     1261 ggcggaggcg agcccgaccg cagggccagc ggccgactgg cgcgcggcga gcgcaaagcc
     1321 gcatgggacg atagggccgg tgccgacccc gatcagggcg aaacccgcga cgctcgccgc
     1381 gaagccggcg tgcgcgctga ccagggcgaa ccctaaggcc gcgatcgcga acgaggccat
     1441 gatgatgcga aggtccctga cgcggaggcg aatggcgtcg acgttgacgc gcagcacggc
     1501 ctggcaggcg gcgaagaacg ccgcgccgag gccggaaatg gcggccagcc gcggcgcctc
     1561 ctcgcgcagg aaaagcgtcg accacagctg ggccgcgagt tccgcggcgg tcgaggcgcc
     1621 aacgacgatc ccaagcccga tcagcgccgg cgcgaaggac aatccgctcc gtctgtttgt
     1681 tccggccggc gaaggcggcg gagggtcgct ccgcgccgcg cgatggtaag cgacgccggc
     1741 gccggcgagc gtgagtgcgg cgaggacgcc ggccgcccac ggcgcggggc ccgcgaaaat
     1801 cacactgccg aggattgcgc cgagcgcgat gccggccgat gcggcggcat gaagccgcgc
     1861 catgatcggg cggccaagcc ggcgctcgat ccgcgcgccc tcggcgttca tgaggacgtc
     1921 gaccacgccg ccaaaccccg agatgacgag cgcaatcgcg acccaggtct tgctcgaggc
     1981 gtcgagcagc gcgcagagcg tggccccata gacgatcgcc gaaacggaga gcgcctgcgt
     2041 gatgccgaag cgctgcgcca acgttccgcc cgccgacatg gcgacgaggt aggcgccaat
     2101 atagacggtc aggaggacgc cgaaggtcgc cgggtcgatg ccggcgtgga cgaggatcgc
     2161 gccggaggcg ccggcccaca ggcccatgcc gacgccgagc gcgaagaaca tcgcggcgac
     2221 cgcgacatgc agggccgcgc cgaccttggc cccgcctggc gtctggacgg cggggttcaa
     2281 aagtcggtcg gggcgccgcc ttccgccttg cggcgggcga cgaaggcgcc aagcgcctcg
     2341 agccgttcgg gctcgagctc tggggcgacg aaatcgcgca agcgcccgac gacgatgcgc
     2401 tcggccgcgt cgagcgcggt cggggaaccc gcttcgcgcc aggtctcgta gttgcgccag
     2461 tcggagatca tcggcgaaaa aaaggcggtg cggtagcgcg cctgggtatg cgctgcgccg
     2521 aagaagtgcc cgccggggcc gacctcgcgc atcgcgtcca gcgccagctc cgattcgtcc
     2581 acgcgcatcg gcttgaggaa ctcggccacc atcgccagaa ggtcggcgtc gagcgccatc
     2641 ttctcgaacg aggcttgcaa gcccccctcc atccagcccg cgccatgcat gagaaagttg
     2701 accccgccca tgatcgcgc
//
LOCUS       DATGJW010000116         5533 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1368787, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000116 DATGJW010000000
VERSION     DATGJW010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5533)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5533)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5533
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1368787"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..983
                     /locus_tag="VKU03_03385"
     CDS             270..983
                     /locus_tag="VKU03_03385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006206776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HLJ70334.1"
                     /translation="MQKAIAEFIGTFTLVFFGCGAAVIGGMGTGPTAINVLGIALAFG
                     LALIAMAYGIGPVSGCHINPAVSFGAFVARRMSLNDLVTYVVAQVVGAIVAALILSLI
                     LSGKAQGWSGGLGQNGWGAGYLGEYNALSAFVFEIVATFLFLVVILGVTHSDTPPHIS
                     GVAGIAIGLTLAVIHIAGINVDGVSVNPARSLGPALVGVGSNPHALAQIWLFIVAPLV
                     GAGAAGLVFANGVIGYKKA"
     gene            1223..3106
                     /locus_tag="VKU03_03390"
     CDS             1223..3106
                     /locus_tag="VKU03_03390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HLJ70335.1"
                     /translation="MLHISELTYRIGQRLLIDEASVALPAGARVGLVGRNGVGKTTLF
                     RLIAGEIAAESGTIALPKRARLGRVEQEAPAGAQSLIDFVLEADKERARLLAEADGAK
                     DAVRIAEIHTRLADIGAHAAPARAAGILAGLGFDDEAQKRPLSEFSGGWRMRVALAAV
                     LFSAPDLLLLDEPTNYLDLEGALWLVDYLAHCPATIIVISHDRDLLDDVAGHILHLDR
                     GKLTLWSGGYTQFERQRCEQRVLQEKALKKQDEKRAHLQSFVDRFRAKASKAKQAQAR
                     IKMLAKLAPVAVLRDEEVLPFRLPPPKKPLSPPIIAMERASVGYGERAVLSRLDLTLS
                     NDDRIGLLGQNGNGKSTFAKLLAGRLAPMSGTVKRASKLEVGFFAQHQIDDLDPAGTP
                     YSHVAALMRGAPEAKIRTRAAQMGFSGAKADTAVSLLSGGEKARLLMGLATFGGPQLV
                     ILDEPTNHLDIDSRAELIEAINDYEGACILVSHDRRLLEACVDRLWLVADGGVKPFDG
                     DLDDYRRFVLAKSAPEASESAVAEIPGKRAAERRETAKARRDLAPLRKDIAAAEDKIR
                     RFADLIARVDEALAKPETFGGDAARALELSRQRGELEKALVAAEEQWLTLAGEAEAVG
                     GAG"
     gene            3364..3855
                     /locus_tag="VKU03_03395"
     CDS             3364..3855
                     /locus_tag="VKU03_03395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70336.1"
                     /translation="MGRWLKAAALGVASLGLTGCNSLFSSLPPPAVAPAAAFPPTLSS
                     AQFIGAWGLASYHNDADRARTEKEAKAQCNKPYVIAQGPNGGLMMHLADQATPSELAL
                     KGADAGRNYIGPANEPAGGADDREIVEVSADSFTTKWVDADTAARYGTMVYARCRGAG
                     KKA"
     gene            3962..4579
                     /locus_tag="VKU03_03400"
     CDS             3962..4579
                     /locus_tag="VKU03_03400"
                     /EC_number="2.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006023022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0018104 - peptidoglycan-protein
                     cross-linking [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HLJ70337.1"
                     /translation="MIRLRALVSFAALAFAAAVSASPAQTVISAPAEIDMNVEDQPGI
                     VPSPDQEQLAGPYEKQVVFYRTSEPPGTIIIVTAERFLYLVQGKNRAIRYGIGVGREG
                     FQWAGLQKISRKQEWPDWRPPPEMIERQPYLPRFMAGGPGNPLGARALYLGQTVYRIH
                     GTNQPETIGHAVSSGCFRLVNNDVIDLYDRVPVGTRVIIKQAASL"
     gene            4590..>5533
                     /locus_tag="VKU03_03405"
     CDS             4590..>5533
                     /locus_tag="VKU03_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HLJ70338.1"
                     /translation="MGPRRRLFALALAALAGVASSAVAGTLEEARQRGALICGVSQGV
                     PGFSIADASGAWSGFDVDFCRAVAAATLGDAAKVRFVPLSADERFDALKAKKIDLLSR
                     NSTWTMGREEALGLVFAGVNYYDGQGFLVTKAKHVDSALELDGAKVCVQSGTTSALNV
                     ADFFEANHMKLDLIVTPTVEELQQAYLGGRCDVMTADMSALYALRTKFAKPEDHVILP
                     DVISKEPLGPAVRQDDMTWFNIVKWVNFALIDAEELGIGTKTIDAALKSEKPDVKRFV
                     GAEGDFGKQLGLAPTWAVAAVTAGGNYGEIFERNLGAKS"
BASE COUNT          953 a         1822 c         1830 g          928 t
ORIGIN      
        1 ccgaaggggg agcgagcgcg aagcggattt cgccgcgctt gccgaactca tccgccgata
       61 tcggccggcc aggccgaaaa acgggcggcg cggcaagatt ttcgaggccg cttgagacgg
      121 gcggcgccat cgaagtgcta agcgggcggg acgcttcggc gcttactttt ttacggctgt
      181 cctcgccttg gcgaatctga tttcttccgc acagcccatt ccgggcggca aggggtgatt
      241 gcgtcaatca aattttacag agggaagcca tgcaaaaagc aattgcggaa ttcatcggca
      301 cgttcacgct cgtcttcttc ggctgcggcg cggcggtaat cggcgggatg gggaccggcc
      361 cgacagcgat caacgtgctt ggcatcgcgc tcgccttcgg cttggcgctc atcgccatgg
      421 cctacgggat cggcccggtc tcgggctgcc acatcaatcc cgcggtgagc ttcggcgctt
      481 tcgtcgccag gcggatgagc ctcaacgatc ttgtcaccta tgtcgtcgcg caggtcgttg
      541 gcgcgatcgt cgccgcgctc atcctcagct tgatcctttc aggcaaagcg caaggctgga
      601 gcggcgggct cggccagaac ggttggggcg ccggctatct cggcgaatac aacgcgctct
      661 ccgctttcgt tttcgaaatt gtggcgacct tcttattcct tgtcgtcatt ctcggcgtga
      721 cgcatagcga cacgccgccg catatatcgg gcgtcgccgg catcgccatc ggcctcaccc
      781 tggcggtcat ccatatcgcg gggatcaatg tcgatggcgt ttcggtcaat ccggcgcgct
      841 cgctcggccc cgccttggtc ggcgtgggca gcaacccgca cgccctcgcg caaatctggc
      901 tgttcatcgt tgcgccattg gtcggcgccg gcgcggcggg gctggtcttc gccaatggcg
      961 tgatcgggta caagaaagcg taaggcgcga cttcccttct ccccgcttcg tgtcgcttcg
     1021 cggggagaag gcggtccgaa gggccggatg aggctgcgca gaactcgaaa ggcaagcgct
     1081 cgccgaaggc caattctcag cgacgcgccg ctgcctattg gcgttcgaga tttgcgccgc
     1141 ttcgcggaat tcgcctaaga gggcagcccg tggccggaag agcgggcttg cgcgtgagcg
     1201 ctcgccgcgc gacaagccgc gcatgttgca tatcagcgag cttacctacc ggatcggcca
     1261 gcgcctgctg atcgacgagg ccagcgtcgc gctgccggcc ggcgcgcggg tcggcctcgt
     1321 cggccgcaac ggcgtcggca agacgacgct gttccggctg atcgccggcg agatcgcggc
     1381 cgaaagcggg accatcgccc tccccaaacg cgcccggctc ggccgcgtcg agcaggaggc
     1441 gccggccggc gcgcagagcc tgatcgattt cgtgcttgaa gccgacaagg aacgggcgcg
     1501 cctgctggcc gaggccgatg gggcgaagga cgccgtacgc atcgctgaaa tccacacccg
     1561 tctcgccgat atcggcgcgc atgcggcgcc ggcccgcgcc gccggcatcc tcgccggcct
     1621 cggcttcgac gacgaggcgc agaagcggcc gctttcggag ttttccggcg gctggcggat
     1681 gcgcgtcgcg ctggcggcgg tgctgttctc ggcccccgac cttttgctgc tcgacgagcc
     1741 gaccaattat ctcgatctcg aaggcgcgct gtggctggtc gactaccttg cgcattgccc
     1801 ggcgacgatc atcgtcatca gccatgatcg cgatttgctc gacgacgtcg cgggccacat
     1861 cctgcatctc gaccgcggca aactgacctt atggagcggc ggctacacgc aattcgagcg
     1921 ccaacgctgc gagcagcggg ttctgcaaga aaaagcgctg aagaagcagg acgagaagcg
     1981 tgcgcacctg caatctttcg tcgatcgctt ccgcgccaag gcgagcaagg ccaagcaggc
     2041 gcaagcgcgc atcaaaatgc tcgccaagct cgcgccggtc gcggtgctgc gcgacgagga
     2101 ggtcctgccc ttccggctgc cgccgccgaa aaagccgctt tcgccgccga tcatcgcgat
     2161 ggagcgggcg agcgtcggct atggcgagcg cgccgtgctg tcgcgtctcg acctgacgct
     2221 ctcgaacgac gatcggatcg gccttctcgg ccagaacggc aacggcaaat cgaccttcgc
     2281 caagctgctc gccggccgcc tcgcgccgat gtccgggacg gtcaagcgcg ccagcaagct
     2341 ggaagtcggc tttttcgcgc agcatcagat cgacgatctc gatccggccg gcacgcctta
     2401 tagccacgtc gccgcgctga tgcgcggcgc gccggaggcg aagatcagga cgcgcgccgc
     2461 gcagatgggt ttttcggggg ccaaggccga caccgccgtc agcctgctct cgggcggcga
     2521 gaaggcgcga ctgctgatgg gccttgcgac cttcggcgga ccgcagctcg tcatcctcga
     2581 cgagccgacc aaccatctcg acatcgacag ccgggccgag ctgatcgagg cgatcaacga
     2641 ttacgaaggc gcttgcatcc tcgtctcgca tgaccggcga ttgctggagg cctgcgtcga
     2701 ccgcctctgg ctggtcgccg atggcggcgt caaacctttc gacggcgacc tcgacgatta
     2761 ccgccgcttc gtgctcgcca aatcggcgcc ggaagcaagc gaaagcgccg tcgccgaaat
     2821 accgggcaag cgcgccgccg agcggcgcga aacggccaaa gcccgccgcg atctcgcgcc
     2881 gctgcgcaag gacatcgccg cggccgagga caagattcgc cgcttcgccg acctgatcgc
     2941 ccgcgtcgac gaagcgctcg ccaagcctga aacgtttggc ggcgacgcgg cgcgagcgct
     3001 cgaattgtcg cgccagcgcg gcgaattgga gaaggccttg gtcgccgccg aggagcaatg
     3061 gctgacgctg gcaggcgagg ccgaggcggt cggcggcgct ggatgatccg ttgtcattcc
     3121 cgcggaaagc ggaaatccag tacggtccac gcggcaagca cggcggattc ccgctttcgc
     3181 gggaatgacg ttgatccatt taaccatgcg aagccggcgc gtcgccctat tgccacggcg
     3241 ggtcgcggtg ctaggctagg cgcgcggcgg tcaaagaagc cgggcccgcc ctcaggggag
     3301 cgcccgccag gccgcgagtc gcgccaagat ggcgcgcgaa gaggacctga cggggagggc
     3361 ctcatggggc ggtggctaaa agcggcggcg ctgggcgtcg cgtctctcgg tttgaccggc
     3421 tgcaattcgc ttttctcgtc tttgccgccg ccggcggtcg cgccggccgc cgctttcccg
     3481 cccactcttt cctcggcgca attcattggc gcctggggcc tcgcctcgta ccacaatgac
     3541 gccgaccgcg cgcgtaccga aaaagaagcc aaggcgcagt gcaacaagcc ctatgtcatc
     3601 gcccaggggc cgaatggcgg cctgatgatg catctcgccg atcaggcgac gcccagcgaa
     3661 ttggcgctga aaggcgccga cgccggccgc aactatatcg gcccggcgaa cgagccggcg
     3721 ggcggcgccg acgatcgtga gatcgtcgag gtttcagccg attccttcac cacgaaatgg
     3781 gtcgatgcgg acaccgccgc gcgctacggc acgatggtct atgcgcgctg caggggcgcc
     3841 ggaaagaagg cttaggcgcg tcgtcgttgc gagcgaagcg aagcaatcca tcgaatggat
     3901 ggcttcgtcg cgtcgctcct cgcaatggcg gcgccaacag cgacaaagag gtccgtcaaa
     3961 catgatccgt ttgcgcgcgc tcgtctcgtt cgccgccctg gctttcgccg cggcggtttc
     4021 ggcctcgccg gcccagaccg tgatttcggc gcccgccgaa atcgatatga acgtcgagga
     4081 tcagcccgga atcgtgccga gtcccgacca ggagcagctc gccggtccgt acgaaaagca
     4141 ggtcgtcttc tatcggacca gcgagccgcc gggcacgatc atcatcgtga ccgccgagcg
     4201 ctttctctat ctcgtgcaag ggaaaaaccg ggcgatccgc tacggaatcg gcgtcggccg
     4261 cgagggattc caatgggcgg gcttgcaaaa gatcagccgc aagcaggaat ggccggattg
     4321 gcggccgccg ccggaaatga tcgagcgcca accgtacctg ccgcgcttca tggccggcgg
     4381 gccgggcaat ccgctcggcg cgcgggcgct ctaccttggc cagacggtct atcgcatcca
     4441 cggcacaaat cagccggaga cgatcggcca cgccgtctcg tcgggctgct tccgcctggt
     4501 caataacgac gtcatcgatc tttacgaccg cgtgccggtc ggaacccgcg tcatcatcaa
     4561 gcaggcggcg agcctttagg gagaccgaca tgggaccgag acgacgcctc ttcgctttgg
     4621 cgctcgccgc gctggcgggc gtggcctcgt ccgccgtcgc cggcacgctg gaggaggcgc
     4681 gccaacgcgg cgcgctgatc tgcggcgtca gccaaggcgt gcccggcttt tcgatcgccg
     4741 acgcgagcgg cgcctggtcg ggcttcgacg tcgatttctg ccgcgcggtg gcggcggcga
     4801 ctctcggcga tgcggccaag gtccgcttcg tgccgctcag cgccgacgag cgcttcgacg
     4861 ccttgaaggc caagaaaatc gatctgctgt cgcgcaactc gacctggacc atgggccgcg
     4921 aagaggcgct cggcctcgtc ttcgccgggg tcaattacta tgacgggcaa ggcttcctgg
     4981 tgacgaaggc caagcatgtc gacagcgcgc tggagctcga cggcgccaag gtctgcgtcc
     5041 agtctgggac gacgagcgcg ctcaatgtcg ccgacttctt tgaggccaat catatgaagc
     5101 tcgacctgat cgtcacgcca acggtcgaag agctgcagca ggcgtatctt ggcggtcgct
     5161 gcgacgtgat gaccgccgac atgtcggccc tctatgcttt gcgcacgaaa ttcgccaagc
     5221 ccgaggacca tgtcatcctt cccgacgtca tctccaagga gccgcttggc ccggcggtgc
     5281 ggcaggacga catgacgtgg ttcaatatcg tcaaatgggt caatttcgcc ctgatcgacg
     5341 ccgaagagtt gggaatcggg accaaaacaa tcgacgccgc gctgaaatcc gagaagcccg
     5401 acgtaaagcg ctttgtcggc gccgagggcg atttcggcaa acaattgggc ctcgcgccga
     5461 cctgggccgt cgcggcggtg acagccggcg gcaattacgg cgagattttc gaacgcaacc
     5521 ttggcgcaaa gtc
//
LOCUS       DATGJW010000117         3517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_851200, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000117 DATGJW010000000
VERSION     DATGJW010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3517
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_851200"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..912)
                     /locus_tag="VKU03_03410"
     CDS             complement(<1..912)
                     /locus_tag="VKU03_03410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MMPL family transporter"
                     /protein_id="HLJ70339.1"
                     /translation="MPNQSPASLESATPSNIAFGIERLGFIGLHLPLVAVLIVALLAI
                     GAAFGVERIKVDDSLSQLFRSNTPQFKQYEEVTKRFPSSEYDVLVVVDGKQLLSRNTI
                     GKLRDFVTDLQLIDGTRGVISLFSARQPAPNGGLPAPLFPEELPQGADYQKLIEQVKS
                     NEIIRGKLLSNDGTLALIVLALDPDVVGGGQLNKVVDEIRKTMAEDLSGANVSAELSG
                     VPIMQLEIRNAVERDRLVYNIIGFVAGCAIAILFFRRVSFMIAAAAPPLLAILLALGA
                     LGWLDFRLNMFLNVMTPLIMVISFSDSM"
     gene            1228..2157
                     /locus_tag="VKU03_03415"
     CDS             1228..2157
                     /locus_tag="VKU03_03415"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ70340.1"
                     /translation="MSAASADASPTLTGIALAATAWAAFSVQDAIVKWLTATVPVPEI
                     LFVRSVGIVALAALALRRSQIAVLLEKGSLVAIGVRSAFILAAWLTYYTAARSLSLPA
                     LVTLYFAAPLFVVALAGVTLGEKPGLARWAATIAGFIGVVIAADFKGAPSVTPALLVL
                     TAALCWAATTILTRSLSLAISTAGLLVGTNFLFIFACGLAAPFVFVRPSAAECGLLLL
                     LVLFGGLGQFFMFQGMRLAPASAVAPFEYSSLAWATLWGWLIFGDLPTPHVLVGGAII
                     LFSGLFMLAAERRRRRAAIAAQGRAAANFTASS"
     gene            2194..2985
                     /locus_tag="VKU03_03420"
     CDS             2194..2985
                     /locus_tag="VKU03_03420"
                     /EC_number="2.7.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003951 - NAD+ kinase activity [Evidence
                     IEA];
                     GO_process: GO:0006741 - NADP biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019674 - NAD metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD kinase"
                     /protein_id="HLJ70341.1"
                     /translation="MQARRGASNNSPAIAFLSSGAPEAEEAKARLVARYGDVKPAKAD
                     VIVALGGDGLMLQALHQTMKAPKPIYGMNRGSVGFLMNEFKETGLIKRIEEARRSIVH
                     PLVMRTIDVVGQTTTARAINEVSLMRQSYQAAKLRILVDGEEQLGELIADGVLVASPA
                     GSTAYNLSVNGPILPLRAPLMALTPISAFRPRRWRGALLPETAHVSIEILEADKRPVA
                     AVADRVEIRTVARVDVSMDLGTDLVLLHDPGHSLEERILREQFRY"
     gene            2998..>3517
                     /locus_tag="VKU03_03425"
     CDS             2998..>3517
                     /locus_tag="VKU03_03425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLJ70342.1"
                     /translation="MTKAKILAEETLADGRFKLAVTTVETTEPDGAKRMLRHEVYHYK
                     HAAALLLYDPQRRVVMLVRQFRVGGYLGGAAQPMTEVCAGMLDGDAPEACVLREAFEE
                     TGIAVSAARHVFDMYTSPGGTTEKIACFVAPYGKRDKKGAGGGVDADEHITLLEPSLD
                     EAIAMIRHGEICD"
BASE COUNT          597 a         1138 c         1125 g          657 t
ORIGIN      
        1 catgctgtcc gaaaaactga tcaccatgat cagcggcgtc atcacgttga gaaacatgtt
       61 gagccggaag tcgagccagc cgagcgcgcc gagcgccagc aagatcgcga gaagcggcgg
      121 cgccgccgcc gcgatcatga acgagacgcg gcggaagaac aggatcgcga tcgcgcagcc
      181 ggcgacgaag ccgataatgt tgtagacgag gcgatcgcgc tcgaccgcgt tgcggatttc
      241 gagctgcatg atcggaacgc cggaaagctc ggcgctgaca ttcgcgccgg aaaggtcctc
      301 ggccatggtc ttgcggattt cgtcgacgac cttgttcaac tggccgccgc cgaccacgtc
      361 gggatcgagc gcgagcacga tcagcgccag cgtgccgtcg ttcgacaaca gcttgccgcg
      421 aatgatctcg ttcgacttca cctgctcgat gagtttttga tagtcggcgc cttgcggcag
      481 ctcctcgggg aaaagcggcg ccggcagtcc gccattgggc gcaggctggc gcgcggaaaa
      541 cagcgagatg acgccgcgcg tgccgtctat gagctgcaga tcggtgacga agtcgcgcag
      601 ctttccgatc gtattgcgcg aaaggagttg cttgccgtca acgacgacga gcacatcgta
      661 ttcgcttgac ggaaaccgct ttgtcacctc ctcgtattgc ttgaattgcg gcgtgttcga
      721 gcgaaacaac tggctgagcg aatcgtcgac cttgatccgc tccacgccga aagcggcgcc
      781 gatggccaaa agggcgacga taaggacggc gaccagcggc agatgcaagc cgataaagcc
      841 gaggcgctcg attccgaagg cgatgttgct cggcgtcgcc gattcgagcg acgccggcga
      901 ttggtttggc aggtcggagg cttcggcgga cagagctttt ctcctcacag aacgggatcg
      961 agcgtcctta acgcggaaga cgggatataa gccgccccga cccgcggaat aaggcctccc
     1021 gttccctgat cggaggaagc atttgcgtga gttcgggaat cgccagatta gcgatctcgg
     1081 cgatggccgc aaagcgccgc ccggcgaata ggcgagcccg cgagcggatt taaggccatt
     1141 tctgggccaa acggccgcgt ctttgcgcct tttgtctcgc gttcaaacct tctatgaacc
     1201 cctttcctcc ttgatcttgg ggctctcttg tccgccgcca gcgccgacgc ctctcccacg
     1261 ctgaccggaa tcgccttggc cgcgaccgcc tgggcggcgt tcagcgtgca ggacgcgatc
     1321 gtcaaatggc tgacggccac ggttccggtg ccggagattc tgttcgtccg cagcgtcggc
     1381 atcgtcgccc tcgccgccct ggcgctgcgc cgctcgcaaa tcgccgtcct gctcgaaaag
     1441 ggaagcctcg tcgcgatcgg cgttcgctcg gccttcatcc tcgccgcctg gctgacctac
     1501 tacacggcgg cgcgctcgct ttcgcttccg gctctggtca cgctctattt cgccgcgccg
     1561 ctcttcgtcg tcgcgcttgc cggggtcacg ctcggcgaaa agcctggcct tgcgcgctgg
     1621 gccgcgacga tcgccggctt catcggcgtg gtcatcgcgg ctgattttaa aggagcgccc
     1681 agcgtcacgc cggctctgct ggttctgacc gccgcgcttt gttgggcggc gacaacgatc
     1741 ctgacgcgaa gcctgagcct ggcgatcagc accgccggcc tgctggttgg aaccaacttc
     1801 ctcttcatct tcgcctgcgg gttggccgcg cctttcgttt tcgtgcggcc gagcgctgcg
     1861 gaatgcggct tgctactcct gctcgtcctt ttcggcgggc tcggacaatt tttcatgttc
     1921 caggggatgc ggctcgcgcc ggcctcggcg gtcgcgccat tcgaatattc ctcgctcgcc
     1981 tgggcgacgc tatggggctg gctgatcttc ggcgatttgc cgacgccgca tgtgcttgtc
     2041 ggcggcgcga tcattctttt cagcgggcta ttcatgctcg cggccgaacg gcgacggcga
     2101 cgggcggcga tcgcagcgca gggccgcgca gccgccaact ttacggcgtc gtcctgatcc
     2161 actatctgtc gccgcctcaa aaaggggccg atcatgcaag cgcggcgcgg cgcgtcgaac
     2221 aattcgccgg cgatcgcctt tctctcctcc ggcgcgccgg aggccgagga ggccaaggcg
     2281 cggctcgttg cgcgctatgg cgacgttaag ccggccaagg ccgatgtgat cgtcgcgctg
     2341 ggcggcgacg gcttgatgct tcaggccctc caccagacca tgaaggcgcc taagccgatc
     2401 tatgggatga atcgcggctc ggtcggcttc ttgatgaacg aattcaagga aaccggcctc
     2461 atcaagcgga tcgaagaagc gcgccgctcg atcgttcatc cgctcgtcat gcgcacgatc
     2521 gacgttgtcg gccagacgac cacggcgcgc gcgatcaacg aagtctcgct gatgcggcaa
     2581 tcctaccagg cggcgaaatt gcgcatcctg gtcgacggcg aagagcagct cggcgaattg
     2641 atcgccgacg gcgtgttggt cgcttcgccg gccggctcga ccgcctataa tctctcggtc
     2701 aacggcccga tcctgccgct ccgcgcgcct ttgatggctc tgacgccgat ctcggctttc
     2761 cggccgcgcc gctggcgcgg cgcgctgctg ccggagaccg ctcatgtctc gatcgaaatc
     2821 ctggaggccg acaagcggcc ggtcgccgcc gtcgccgatc gcgtcgaaat ccggaccgtc
     2881 gcgcgcgtcg acgtctccat ggatctcggc accgatctcg tcctgctgca cgatcccggc
     2941 cattcgctcg aagagcgcat cttgcgcgag caatttcgct attgagagct tggcgcgatg
     3001 acaaaggcca aaatactcgc cgaagagacg ctcgccgacg gccgcttcaa gctcgcggta
     3061 acaacggtcg agacaaccga gcccgacggc gcaaagcgca tgctgcgcca cgaggtttac
     3121 cattacaagc atgcggcggc gttgctgcta tatgatccgc agcgccgcgt cgtgatgctg
     3181 gtgcgccagt tccgcgtcgg cggctatctc ggcggcgctg cgcagccgat gaccgaagtc
     3241 tgcgccggca tgctcgacgg cgacgcgccg gaggcttgcg tcctgcgcga agctttcgag
     3301 gagaccggaa tcgcggtttc cgccgcgcgc catgtcttcg atatgtatac cagccccggc
     3361 ggcacgacgg agaagatcgc ctgcttcgtc gcgccctatg gcaagaggga taagaagggc
     3421 gcgggcggcg gcgtcgacgc ggacgagcac atcacgctgc ttgagccttc gctggacgag
     3481 gcgatcgcaa tgatccggca cggcgaaatc tgcgacg
//
LOCUS       DATGJW010000118         7748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_747276, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000118 DATGJW010000000
VERSION     DATGJW010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7748
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_747276"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..401)
                     /locus_tag="VKU03_03430"
     CDS             complement(195..401)
                     /locus_tag="VKU03_03430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70343.1"
                     /translation="MKKIVLAAAALAVGLGVAPAFADDAAQPAPAPMVHHHHHHHHHM
                     MRHHHHHHHHHHHHMMKKEEPKKS"
     gene            complement(579..1433)
                     /locus_tag="VKU03_03435"
     CDS             complement(579..1433)
                     /locus_tag="VKU03_03435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70344.1"
                     /translation="MSAGAPLTQALAYVDDCLAPEERRDFERRMAADPALAARIEQWR
                     RQNEAIRQAFADTAPAEGAPPIATVPPPLRRDFGRRRQSPFEADAPGRSLAGPGAPPQ
                     PQEIARPAPWRRLPRRAFVLFGAALALTLSAAPAPRDASAELAGAAFSAFRTFAEGGA
                     PEFATTETAALETWLRRRVGGWLALPDLGAAGFTLVGGRVISGVEGPAAFALYRDRAG
                     ARLGLTVESSEAAAPPLIRASGALVATALAAPGAEQATLVAVKDEVDIGGLARRARFA
                     TPERYATP"
     gene            complement(1430..1948)
                     /locus_tag="VKU03_03440"
     CDS             complement(1430..1948)
                     /locus_tag="VKU03_03440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004117935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma factor-like helix-turn-helix DNA-binding
                     protein"
                     /protein_id="HLJ70345.1"
                     /translation="MLGSTAEWREEARALRRFAIALASDARFARDRAAATLVADSLVN
                     RSLLAFHNGEGPGELSRRLRLLCLLVRFHRRHSRLRGFDEDCPESGFAGGAPMERAVA
                     ALPAEWREALLLVAIERLSHQQAAAVLEISLAALLDRLARARAALSQTLTGPAAAPLE
                     RRNAAPHLRLIK"
     gene            2153..2992
                     /gene="fabI"
                     /locus_tag="VKU03_03445"
     CDS             2153..2992
                     /gene="fabI"
                     /locus_tag="VKU03_03445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004318 - enoyl-[acyl-carrier-protein]
                     reductase (NADH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HLJ70346.1"
                     /translation="MAEGEKADLPVAGPLKGMRGIVLGVANNRSIAYGIAKAARAAGA
                     DLALTFQGEALEKRVRPLAQELGAEVVGHCDVTDAASIDAVFAATEKLWGGLDFLVHC
                     IAFSDKDQLTGRYIDTTEDNFAKSLLISCYSFTALAQRAEKLMTKGGSMLTLTYYGAE
                     KWMPHYNVMGVAKAALEASVRYLAADLGEKAIRVNAISAGPIKTLAASGIGDFRYILR
                     WNEYNAPLRRTVTIEDVGQSAVYLLSPMSSGVTGEILHVDAGYHIVGMKNPAAPDISV
                     VKD"
     gene            3144..3815
                     /locus_tag="VKU03_03450"
     CDS             3144..3815
                     /locus_tag="VKU03_03450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70347.1"
                     /translation="MAIALLVVAGAFANAGRRLRAPLRVARPGGAAAGFMVVIWLLAI
                     YTAGVATSVYGLQVKQAYPGFVAQHVRVGTFLDALIAFFAILYLTRGWGWRVALASAV
                     VGAAAAPMIFEFPFDLIVMTRTNPPMPMLYRQLFFLPLFLVEFSTLSLLTLLPSVRVT
                     APAAYAVAGMFVVFAVWAAFGFAFPAEPLPLALNVISKILCFVAAVMLFVWRADGEAA
                     GSRAG"
     gene            complement(3869..5050)
                     /locus_tag="VKU03_03455"
     CDS             complement(3869..5050)
                     /locus_tag="VKU03_03455"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70348.1"
                     /translation="MKPYSAIAALLASAFLLIAGNGLVNVLTPLRAAIEGFPELTVGL
                     LGSVYFGGMLAGTLATSPLVRRAGHIRAFSALVAGAVVTVILLPAIVNPIAWLVLRGA
                     LGFVFAGLYSVIESWINAKATNSNRGALYGFYQIVAYAASAGGQMALTLQAPTSFIGF
                     TIGGALLALSTIPLAMTNADAPIPPRVVRLRLIWLMRLSPVGALAAFLVGATNGAYSA
                     LGPVYALGVGFTPQAAPLFTTAVTIGSALGVYPAGRVSDRVDRRAMIAVLSGVGALIE
                     IALWRFHGGQTALVALGFAVGATTFTLYTLTTSHANDRAKAEHAVLVSTGLLFLYCAG
                     AIIGPIVAAALMRNGRPGMLYAQNATLHLGLAAFALWRYVYTRRARAQVKKAAAELAD
                     A"
     gene            5273..5473
                     /locus_tag="VKU03_03460"
     CDS             5273..5473
                     /locus_tag="VKU03_03460"
                     /inference="COORDINATES: protein motif:HMM:NF021474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HLJ70349.1"
                     /translation="MATNLSIDPDLIEQALEVSGERTKKAAVTKALQEFVARRRQKRL
                     LDLLGRLEWDSSYNYKTERTRD"
     gene            5470..5889
                     /locus_tag="VKU03_03465"
     CDS             5470..5889
                     /locus_tag="VKU03_03465"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLJ70350.1"
                     /translation="MSLFIDTSVWSLAFRRDSPASAEAAALVRAIESGETILTTGLVL
                     QELLQGFSGPKARIQILDRFSAVPLLTPDRDDHVRAAELRNHCRRNGVQIGTIDALLA
                     RLCIRHDLIMLTADSDFRRIRAHSALRLWDQEQASRL"
     gene            6024..7412
                     /locus_tag="VKU03_03470"
     CDS             6024..7412
                     /locus_tag="VKU03_03470"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase class II"
                     /protein_id="HLJ70351.1"
                     /translation="MAESRWSPESWRSKPVEQAPAYPDPRALAEVEKQLAGFPPLVFA
                     GEARKLKRALGKAAAGEAFLLQGGDCAESFAEHSADNIRDFFRVFLQMAVVMTFAGSS
                     PVVKVGRIAGQFAKPRSNATEKIGGVELPSYRGDIINDIGFDAASREPDPRRQLEAYR
                     QSAATLNLLRAFANGGYANLENVHRWMLGFVKDSPQSGRYQELADRITETLGFMRAIG
                     LDTEAHPELRATDFYTSHEALLLGFEQAMTRVDSTTGDWYATSGHMIWVGDRTRQLDG
                     AHVEYCRGVKNPIGLKCGPSLKPDDLIRLIDALDPDGEPGRLTLICRFGADKVEAHLP
                     PLIRAVKREGRKVLWSCDPMHGNTIKSASNYKTRPFERIVGEIKSFFAVHRGEGTYAG
                     GVHLEMTGKDVTECTGGARAISEADLHDRYHTYCDPRLNAEQAIEVAFLIAELLKAER
                     LAAGVKHVDAAE"
BASE COUNT         1356 a         2505 c         2627 g         1260 t
ORIGIN      
        1 gccgtacgta ccgaagacgg aacgttgtcg tttcccggaa agcgggaatc cggagagccg
       61 ccgcgcgata ggtcgtactg gattcccgct tgcgcgggaa tgatacggag gatcgcgcga
      121 aaaagaaaaa tcccggtccg ctcgtcgcgg ccgggatttc gtccccaaaa aaagctcgta
      181 ggctggcgcc gcgctcagct cttcttcggc tcttccttct tcatcatgtg atgatgatga
      241 tgatggtggt gatgatgatg atggcgcatc atatggtgat gatgatgatg gtggtgatga
      301 accatcggcg ccggcgcggg ctgcgcggcg tcgtcggcga aagccggcgc gacgcccaag
      361 ccgaccgcga gcgcagccgc ggcaagaacg atcttcttca ttgtcttaga attccccttt
      421 tatcctcgga agcgaggatc gcgccgaaag tgcatgaacg aagatgaact caacatgaat
      481 aagcagatga ttttcctggg gaaaatctcg atttaagaca gcccgccggc aagccgttca
      541 aggcgcgccg atcgtgcgcg agcaattatc gccaattatc aaggcgttgc gtaccgctcc
      601 ggcgtcgcga aacgggcgcg gcgggccaag ccgccgatat cgacttcgtc cttgacggcg
      661 acgagcgtcg cttgctccgc gcccggcgcg gccaaggcgg tcgcgaccag ggcgccgctg
      721 gcgcgaatga gcggcggcgc cgccgcttcg ctcgattcga cggtcaagcc gaggcgcgcg
      781 ccggcgcgat cgcggtagag cgcaaaggcg gccggtcctt ccacgcccga aatcacgcgc
      841 ccgccgacca gcgtgaagcc ggctgcgccg agatcgggaa gggcgagcca gccgccgacc
      901 cggcgccgca gccatgtctc cagcgcggcg gtttccgtcg ttgcgaattc cggcgcgccg
      961 ccctcggcga aagtccggaa ggcggaaaac gccgcgccgg ccagctccgc ggaagcgtcg
     1021 cgcggcgccg gcgcggcgct caacgtcagg gcgagagcgg cgccgaaaag cacaaaagcg
     1081 cgccgcggca aacgccgcca cggcgccggc ctcgcgatct cctgaggctg tggcggcgcg
     1141 ccgggcccgg cgaggctgcg ccctggcgcg tccgcctcga acggcgattg ccggcgccgg
     1201 ccgaaatcac ggcgcagcgg cggcgggacg gtcgcgatcg gcggcgcgcc ctcggcaggg
     1261 gcggtatcgg cgaaggcctg gcggatcgcc tcgttctggc ggcgccattg ctcgatccgc
     1321 gcggcgagag ccggatcggc cgccattcgc cgctcgaagt cgcgccgctc ttccggcgcg
     1381 agacaatcgt ccacataggc caaagcctgc gtcaaaggcg cgccggcgct cacttgatca
     1441 gcctcaaatg gggcgctgcg ttgcgacgct ccaacggagc ggccgccggc cccgtcaaag
     1501 tttgcgagag cgcggcgcgg gcgcgggcga gccggtcgag cagcgccgcc agcgagattt
     1561 ccagcacggc cgccgcctgc tggtgcgaca ggcgctcgat cgccacgagc aacagcgctt
     1621 cccgccattc ggcaggcagg gccgcgacgg cgcgctccat cggcgcgccg ccggcaaagc
     1681 cgctttccgg gcagtcctca tcgaaaccgc gcaagcggct gtgacggcga tggaagcgca
     1741 ccagcaggca gagcaggcgc aaacgccggc tcaactcccc cggtccctcg ccattgtgaa
     1801 aagccaaaag cgagcggttg accaggctgt cggcgacgag cgtcgccgca gcccggtcgc
     1861 gcgcgaagcg cgcatccgac gccaaggcga tggcgaagcg cctgagcgcc cgcgcctcct
     1921 cccgccactc ggccgtcgag cccagcatcg cgtcccacgc gaattatcct taatgaaatc
     1981 tagaccgacc tgaggtccgc tccaagcgct ctgtgaaatt tcacacattt tttgtgaggc
     2041 gaacggcgtt cggcgggccc ttcccagcgg cgggcgcgga cgataaagga tgcgctttga
     2101 gggcgcagat cggccgagtc gagggtcctt catgaatatg gcgaggtttt cgatggctga
     2161 gggcgagaag gcggacttgc cggtcgccgg cccgctcaag gggatgcgcg ggatcgtcct
     2221 cggcgtcgcc aacaaccggt cgatcgccta tggcatcgcc aaggcggcgc gcgcggcggg
     2281 cgccgatctg gcgctgacct ttcagggcga agccttggag aagcgcgtgc gcccgctcgc
     2341 gcaggagctc ggggccgaag tggtcggcca ttgcgatgtg accgatgcgg cctcgatcga
     2401 cgcggtcttc gccgcgaccg agaagctctg gggcggcctc gacttcctcg tccattgcat
     2461 cgccttctcg gacaaggacc aattgaccgg ccgctatatc gacacgaccg aggacaattt
     2521 cgccaagtcg ctgctgatct cctgctattc gttcaccgct ctcgcccagc gcgccgaaaa
     2581 gctgatgacg aagggcggct cgatgctgac cctgacttat tacggcgccg aaaaatggat
     2641 gccgcattac aacgtgatgg gcgtcgccaa ggccgcgctg gaggcgagcg tgcgctatct
     2701 cgccgccgat ctcggcgaga aggcgatccg ggtcaacgcc atctcggccg ggccgatcaa
     2761 gacgctcgca gcttcgggaa tcggcgattt ccgctacatc ctgcgctgga acgaatacaa
     2821 cgcgccgctc cgtcgcaccg tgacgatcga ggatgtcggc cagagcgcgg tctacctgct
     2881 gtcgccgatg tcgagcggcg tcacgggcga aatcctccat gtcgacgcgg ggtaccatat
     2941 cgtcggcatg aagaacccgg ccgcgccgga tatttcggtg gtgaaggact gaccattagg
     3001 cgtctcgatc gatgagatcg gggatacaag acgggctggc gcgcgccgac tcgttggtcc
     3061 ggcttgcgca ggctcaaggt tccgcgccgt cgtctcatgc ggccgcacac tgggtttcct
     3121 atagcggcgg cgcggcgatc ctcttggcga tcgcgctgct cgtcgtcgcc ggcgcatttg
     3181 ccaatgccgg ccgacgcctg cgcgccccgc tcagggtcgc gcggccgggc ggcgccgccg
     3241 ccggattcat ggtcgtgatc tggctgctgg cgatctacac cgccggcgtc gcgacgtccg
     3301 tctatggatt gcaggtgaaa caagcctatc cgggctttgt cgcgcagcat gtgcgcgtcg
     3361 ggaccttcct cgatgcgctc atagccttct tcgcaattct ctacctcacg cggggttggg
     3421 gctggagggt cgccctcgcc agcgccgtcg tcggcgccgc cgccgcgccg atgattttcg
     3481 agtttccttt cgatctgatc gtcatgacgc ggacgaatcc gccgatgcca atgctgtacc
     3541 ggcagctttt cttcctgccg ttgttcctgg tcgaattctc gacgctatcg ctactgacct
     3601 tgctgccttc cgtgcgggtg accgcgcccg ccgcttatgc ggtggcgggt atgttcgtcg
     3661 tattcgccgt ctgggctgcg ttcgggttcg cctttcccgc cgagccgttg ccactcgcgc
     3721 tgaacgtcat ttcgaaaatc ctctgcttcg tggcggcggt catgctgttt gtctggaggg
     3781 cggacgggga agccgctgga tctcgcgcag gatgacgcgc gcttgacggc acggcgagga
     3841 tagcacggac cgcctggcct gcgagccgct aggcgtcggc caattctgca gccgccttct
     3901 tgacctgcgc gcgagcgcgg cgcgtataga cgtaccgcca cagcgcgaaa gcggcgagcc
     3961 cgagatgcag ggtcgcgttc tgcgcataga gcatgccggg gcggccgtta cgcatcaaag
     4021 cggcggcgac gatcggcccg atgatcgcgc cggcacagta gagaaagagc aggccggtcg
     4081 agaccagcac cgcgtgctcg gccttggcgc ggtcattggc gtgcgatgtc gtcagcgtat
     4141 agagcgtgaa ggtggtggcg ccgaccgcga agccgagcgc gaccaacgcg gtctggccgc
     4201 catgaaagcg ccagagcgcg atttcgatca gcgcgccgac gccggagagg accgcgatca
     4261 tcgcgcgccg gtcgacgcgg tccgacacgc ggccagccgg atagacgccg agcgccgagc
     4321 cgatcgtcac ggcggtggtg aagaggggcg ccgcctgcgg cgtgaagccg acgcccaaag
     4381 catagaccgg gccgagcgcc gaataggcgc cgttggtcgc gccgacgaga aaggcggcga
     4441 gcgcgccgac cggcgacagg cgcatcagcc agatcaggcg cagccggacc acgcgcggcg
     4501 ggatcggcgc gtcggcgttg gtcatggcga gcggaatggt cgagagcgcc agcagcgcgc
     4561 cgccgatcgt gaagccgata aaggaggtcg gcgcttgcag ggtcagcgcc atttggccgc
     4621 cggccgaggc tgcataggcg acgatctggt agaagccgta aagcgcgccc cggtttgaat
     4681 tggtggcctt ggcgttgatc caggattcga tcaccgaata gaggccggcg aagacgaagc
     4741 cgagcgcgcc gcgcagaacg agccaggcga tcgggttgac gatcgccggc agcaaaatca
     4801 ccgtgaccac cgcgccggcg acgagcgccg aaaaggcgcg gatatggccg gcccgccgga
     4861 cgagcggcga cgtcgccaga gtgcccgcca gcatgccgcc gaaatagacc gagccgagca
     4921 ggccgacggt cagttcggga aagccctcga tcgcggcgcg cagcggcgta aggacgttga
     4981 cgaggccatt gccggcgatg agcagaaagg ccgaagcgag cagcgcggcg atggcggagt
     5041 agggtttcaa gacgcgattc gaatcaaagg cgggcgcgcc atgctaggcg acgcgcggcc
     5101 gtcgagatag ggcgacggcg attgggctgg gctgcgtcgg ttagagaaac ccggctcgaa
     5161 gcgcgctcgg cggatttcgc ttccagcgca aacgatccca tggacccgcc gcccttgccg
     5221 gcgcgctgtt cgcaggcgcg aattgtgtgt tatgatgtgt agaggctcac acatggcgac
     5281 aaacctgtca atcgaccccg acctgatcga gcaagcgctg gaggtcagcg gcgagcgcac
     5341 caaaaaggcc gctgtcacga aggcgttgca ggaattcgtg gcgcggcggc gccagaagcg
     5401 cctgctcgat ctgctcggga ggctcgaatg ggattcctct tataattata agactgagcg
     5461 gacgcgcgat tgagcctttt catcgacacc agcgtctggt cgctggcgtt tcggcgcgat
     5521 tcgcctgcct cggcggaagc cgcggcgctg gtcagagcca ttgaaagcgg cgagacgatc
     5581 ctgacgaccg gcctcgtcct tcaggagctg ctgcaaggtt tttcaggccc gaaggctcgc
     5641 attcagatcc tggatcggtt ttccgcggtc cccttgttga cgccggatcg cgacgatcat
     5701 gtcagggcgg ccgaattgcg gaatcattgc cgccgaaacg gcgtacagat cggaacgatc
     5761 gacgctctgc tggctcgatt gtgcatccgc cacgacctga tcatgctgac ggccgacagc
     5821 gatttcaggc gcattcgcgc tcactccgcg ctccggctgt gggatcagga gcaggcgtcc
     5881 cgcctatgaa ggggcagccc ggcttcctct cgagcgtctg catatcgcca agccgccgcc
     5941 atggtgtata agccggccaa agagccgccg cggatggatt tgagcgatcg cggcgagccg
     6001 caactttagt caaggcgtcg atgatggccg aaagccgctg gagtcccgaa agctggagat
     6061 cgaagccggt cgagcaggcg ccggcctatc ccgatccgcg cgcgctcgcc gaggtcgaga
     6121 agcaactcgc cggctttccg ccgctggtct tcgccggcga ggcgcgcaag ctgaaacggg
     6181 cgctgggcaa ggcggcggcg ggcgaggctt tcctgctgca gggcggcgat tgcgccgaga
     6241 gcttcgccga gcattcggca gacaatatcc gcgacttctt ccgggtgttc ctgcagatgg
     6301 cggtggtgat gaccttcgcc ggctcatcgc cggtggtgaa ggtcggccgc atcgccggcc
     6361 agttcgccaa gccgcgctcc aacgcgaccg aaaagatcgg cggcgtcgaa ttgccgagct
     6421 atcgcggcga catcatcaac gatatcggct tcgatgcggc ttcgcgcgag cccgatccgc
     6481 gccggcaatt ggaggcctat cgccaatcgg cggcgacgct gaaccttttg cgcgccttcg
     6541 ccaatggcgg ctacgccaat ctcgaaaacg tccatcgctg gatgctcggc ttcgtcaagg
     6601 acagtccgca atcgggccgc taccaggagc tcgccgaccg catcaccgag acgctcggct
     6661 tcatgcgcgc gatcggcctc gacaccgaag cccatcctga attgcgggcg acggatttct
     6721 acacctcgca cgaggcgctg ctgctcggct tcgagcaggc gatgacgcgc gtcgattcga
     6781 cgaccggcga ttggtacgcg acctccggcc atatgatttg ggtcggcgac cgcacgcgtc
     6841 agctcgacgg ggcgcacgtc gaatattgcc gcggcgtcaa aaatccaatc ggcctcaaat
     6901 gcggcccgag cctcaagccg gacgatttga tccgcctcat cgacgcgctc gatcccgatg
     6961 gggagccggg ccggctgacg ctgatctgcc gcttcggcgc cgacaaggtc gaggcgcatt
     7021 tgccgccgct gatccgcgcg gtgaagcgcg aaggccgcaa agtgctgtgg tcctgcgatc
     7081 cgatgcatgg caacacgatc aagtcggcgt cgaactacaa gacgcggccg ttcgagcgca
     7141 tcgtcggcga gatcaagagc ttcttcgccg tccatcgcgg cgagggaacg tacgccggcg
     7201 gcgtgcatct cgaaatgacc ggcaaggacg tcaccgaatg caccggcggc gcccgcgcga
     7261 tcagcgaggc cgacttgcac gaccgctatc acacgtattg cgacccgcgg ctcaacgccg
     7321 agcaggcgat cgaggtcgct ttcctgatcg ccgagctgct gaaagccgag cgcctggccg
     7381 ccggcgtcaa gcatgtcgac gcggcggagt aggggctaag atttcccgtt gaaaatgagg
     7441 gctcgtccgc gggagggcca acggcgcttg ggcccccctc tccccgctgc gcgcaggggc
     7501 tgtccgggga aagtctgagg ttgtcacggc gtctgagacg taacctttca gcgccgcggc
     7561 gccacgccgg ccgatcgccg tcaacccgcc atagcgcctc ccgagttacg cacaaacgaa
     7621 cgcccgatcg tgcggcgcta tggcgggtcc ttcacttcgt tccggccctt cgggtgactg
     7681 cgatcgtccg gcgtggctta ttggctacca agacgttttg ctgcccgaaa cggccgaacg
     7741 gccggttt
//
LOCUS       DATGJW010000119         5267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_436180, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000119 DATGJW010000000
VERSION     DATGJW010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_436180"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..239)
                     /locus_tag="VKU03_03475"
     tRNA            complement(163..239)
                     /locus_tag="VKU03_03475"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(203..205),aa:Arg,seq:acg)
     gene            complement(370..1866)
                     /locus_tag="VKU03_03480"
     CDS             complement(370..1866)
                     /locus_tag="VKU03_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLJ70352.1"
                     /translation="MLDATPAAVLEQAPDADAAGGLAPSRRFSLGLAARLLLLTIGIV
                     LITQVIMFVPRVAAYRESQLRDRLAAADTAALVFAAAPDGMLSKELTKQILDSVGAQT
                     IAIKTRYTRRLLAMADTPPPVTDSYDLRNASTVESIAAAFRGFFAPKGAVLKVVGKAP
                     MGGDFLEITLDEKRITDVVWGYAGRFLRASLIISGAIGLVLWTAIWLMVLRPVRRLTS
                     NIIAFGEKPEDETRVITPSGQGHEIGRAEEALAAMQRSLGRELDQKKRLAQLGLAVAK
                     ISHDLRNMLTAAQLISDRLATIPDPLAMRLGPRLVATLDRAIAFCQETLSYGGAGEKP
                     PSPRAFALGALLSELAEVAHAKQTGGIDVRMEIPDDFEIYADPDHVLRMIGNLISNAV
                     QALQQHGPTEGKPSAIRVRAARQGETALIEVSDTGPGVPTQLAPRIFEPFHKSTRPGG
                     SGLGLAIAAELVDRNGGAITLAPSPPGDFYSGACFQLTLPLAQKKAAA"
     gene            complement(1868..2860)
                     /gene="denD"
                     /locus_tag="VKU03_03485"
     CDS             complement(1868..2860)
                     /gene="denD"
                     /locus_tag="VKU03_03485"
                     /EC_number="1.1.1.410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019965192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-erythronate dehydrogenase"
                     /protein_id="HLJ70353.1"
                     /translation="MKILIIGAAGMIGRKLAERIAKDGALNGSAIESADLVDIVAPSP
                     PAGAKFKIACQAADISDPAVAPRLVAARPDAIFHLAAIVSGEAEQNFDKGYAINLDGT
                     RYLFEAIRQENLRSGGRYAPRVVFASSIAVYGAPFHETIEDEFFLTPLTSYGTQKAIG
                     ELLLSDYSRRGFFDGVGIRLPTICVRPGRPNLAASGFFSNIIREPLVGQPAVLPVSED
                     VRHTHASPRSAVGFLLHAAALDTGPLGARRNLSMPGISVTVGQQIEALRKVAGEKAAS
                     RIRREPDETIARIVAGWPRRIEAKRARELGFTAESNFEEIIRVHIEDELDGKIA"
     gene            3094..3408
                     /locus_tag="VKU03_03490"
     CDS             3094..3408
                     /locus_tag="VKU03_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="septal ring lytic transglycosylase RlpA family
                     protein"
                     /protein_id="HLJ70354.1"
                     /translation="MKPILLALLLAATASVEANAQSVGQASYYNHPRYRGLIAAHRSF
                     PLGTHVRVVNLSNGRAATVVIVDRGPFIRSRIIDVSTRAAEILGFRRAGVARVRLEVV
                     NK"
     gene            complement(3415..5037)
                     /locus_tag="VKU03_03495"
     CDS             complement(3415..5037)
                     /locus_tag="VKU03_03495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ70355.1"
                     /translation="MNQAVASIEALLGDAVATVRARVADGGRLSAALIEREQRAAHGL
                     AWLATYASAAREIVSYARRLRRERRFGPAEALLTAIGLGEFLAQIFGGIPMSQGEIVR
                     LGDFGLGARQIAARRDGAAAALMEENNPANRRALADLLADSHSTTFGDPGLDDTLDAI
                     RAEMRRFAEAEIAPHAQAWHRANAYIPLDVIARLANMGVFGLTIPEAYGGMGLGKVAM
                     CVVTEELSRGYIGVGSLGTRSEIAAELILSGGTEAQKREFLPKIASGEILPTAVFTEP
                     NTGSDLASLATRAVRDGDVYRIHGAKTWITHPVRADLMTLLARTDPSEKGYRGLSMFL
                     ARKPRGGDAEPFPAPGMSGGEIEVLGYRGMKEFEIAFDGFQIPASGLLGGQEGLGFKQ
                     LMATFESARIQTAARAVGVAQSALELGLAYAKERRQFGKPLIAFPRVADKLVMMAVEI
                     HIARQIAYFAARAKDESRRCDVEAGMAKLLAARVAWAAADNALQIHGGNGFALEYPIS
                     RVLCDARVLNIFEGAAEIQAQVIARRLLDEAN"
BASE COUNT          848 a         1708 c         1814 g          897 t
ORIGIN      
        1 tggcttgggg caaaatccac tttttcatcc cgaagcgatt cgaacttttt caccgccaac
       61 accgccgctt ctacgctttt ttcgcccgtc agatatctcc cgcttccacg tgggcgtagt
      121 ttcggcggtc atcgacggct atagagggca atcggcggga tttggcgcgc ccgaagagat
      181 tcgaactcct gacccccaga ttcgtagtct ggtgctctat ccagctgagc tacgggcgcc
      241 tgccgcgctc cttaagcttc ccaggcgcct ctgagcgagg cgagggaggg tcggaaattt
      301 gcggcgcgct ccctctagcc gctctcgccg gaggaggcaa gcgcggcgtc gcggcggcgg
      361 ccgccaaagt caggccgcgg cctttttttg cgcgagaggc agggtgagct ggaagcacgc
      421 gccgctgtag aaatcgccgg gcgggctcgg cgcgagcgtg atggcgccgc cattgcggtc
      481 gacgagctcg gcggcgatgg cgaggccgag gcccgagccg ccgggccggg tcgatttgtg
      541 gaacggctcg aaaatgcgcg gcgccagttg cgtgggaacg ccgggtccgg tgtcgctgac
      601 ttcgatcagc gccgtctcgc cctgccgcgc cgcccggacg cggatcgcgc tcggcttgcc
      661 ctccgtcggt ccgtgctgct gcagggcctg aacggcgttg ctgatcaggt tgccgatcat
      721 tctaagcaca tggtcgggat cggcgtagat ttcgaaatcg tccggaatct ccatgcgcac
      781 gtcgatgccg ccggtctgct tggcgtgcgc gacttcggcg agctccgaaa gaagcgcgcc
      841 gagcgcgaag gcgcgcggcg acggcggctt ctcgccggcc ccgccatagg agagcgtctc
      901 ctggcagaag gcgatggcgc ggtcgagcgt ggcgacgagg cgcgggccga gccgcatcgc
      961 cagcggatcg gggatcgtcg caagccggtc cgagatcaat tgcgcggcgg tcagcatatt
     1021 gcgcagatcg tggctgatct tggcgaccgc caggccgagt tgcgccaagc gcttcttctg
     1081 gtcgagctcc cggccgaggc tgcgctgcat cgccgctagc gcctcctcgg cgcggccgat
     1141 ctcgtggccc tggccgctcg gcgtgatgac gcgggtctcg tcctccggct tttcgccgaa
     1201 ggcgatgatg ttcgaggtca ggcggcggac cggccgcagc accatcagcc agatcgccgt
     1261 ccagaggacc aggccgatcg ccccggatat gatcagcgat gcgcgcagga agcgcccggc
     1321 atagccccag accacgtccg tgatgcgctt ttcgtcgagc gtgatttcga ggaagtcgcc
     1381 gcccatcggc gccttgccga cgactttgag caccgcgcct ttcggcgcga agaagccgcg
     1441 aaaggcggcg gcgatcgact ccaccgtcga ggcgttgcgc agatcgtagg aatcggtcac
     1501 cggcggcggc gtgtcggcca tcgccagcag gcggcgggtg tagcgggtct tgatggcgat
     1561 ggtctgggcg ccgacgctgt cgagaatctg cttggtcagc tctttcgaca gcatgccgtc
     1621 cggcgccgcg gcgaagacca aagccgccgt atcggccgcc gccagccggt cgcgcaattg
     1681 cgattcgcga taggcggcga cgcgcggcac gaacatgatc acctgggtga tcaggacgat
     1741 gccgatcgtc aggagcagca ggcgggcggc gaggccgagg ctgaaccggc gcgaaggggc
     1801 gagcccgccc gccgcgtctg cgtcaggcgc ctgttccaga acagcggcgg gcgtcgcgtc
     1861 aagcatgtca ggcgatctta ccgtcgagct cgtcttcgat atgaacgcga atgatctcct
     1921 cgaaattgga ttcggccgta aagccgagct cgcgggcgcg cttggcctcg atccggcgcg
     1981 gccagccggc gacgatgcgg gcgatcgtct cgtccggctc gcggcgaatg cggctcgccg
     2041 ccttttcgcc ggcgactttg cgcaacgctt cgatctgctg gcccaccgtc accgaaattc
     2101 ccggcatgct caaattgcgc cgcgcgccga gcggtcccgt atccagcgcc gccgcgtgca
     2161 gcagaaagcc gaccgccgag cgcggcgagg cgtgcgtgtg gcgcacatcc tcggagaccg
     2221 gcagcacggc cggctgaccg acaagcggct cgcggatgat attggagaag aaacccgatg
     2281 cggcaaggtt cggccggccg ggtctcacgc atattgtcgg cagcctgatg cccactccgt
     2341 cgaaaaagcc gcgtcgcgag taatcggata aaagcaactc gccgatggct ttctgcgttc
     2401 cgtagctggt gagcggggtt aggaaaaact cgtcctcgat cgtctcgtgg aaaggcgcgc
     2461 catagacggc gatcgaagag gcgaagacga cgcgcggcgc gtagcgcccg ccgctgcgca
     2521 gattttcctg ccggatcgcc tcgaacaggt agcgcgttcc gtcgagattg atcgcatagc
     2581 ccttgtcgaa attctgctcg gcctcgccgg agacgatggc cgcgaggtgg aaaatcgcgt
     2641 ccggccgcgc cgcgacgagg cggggcgcga cggccggatc ggaaatgtcc gccgcctggc
     2701 aggcaatttt gaattttgcg ccggccggcg gggacggcgc gacaatatcg acgagatcgg
     2761 cgctttcgat cgccgatccg ttcaaggcgc cgtccttggc gattcgttcg gcgagcttgc
     2821 ggccgatcat gccggctgcg ccaataatga gaattttcaa ggcattggct cccataaggc
     2881 tctgcgcaaa aggcgtttgt ccctgctcgg ctttatagag cgacggcgct cattcaacca
     2941 gcgctggaag cccatgtttc cccctgccgg gcggctgatc tgcgctgcgg tcaggtcaaa
     3001 gctcgatttc ggcccatccg cgccgctttt tcgacgcttt cctttggact tgtgagaggg
     3061 gcggggaggc gagagataaa aggggcgccc tgcgtgaagc cgatcctgct cgcacttttg
     3121 ctcgccgcca cggcgagcgt cgaagccaac gcccaatcgg tcggccaagc gtcctattac
     3181 aaccatccgc gctatcgcgg cctgatcgcc gcgcatcgca gcttcccgct cggcacgcat
     3241 gtgcgcgtcg tcaatttgag caatgggcgc gccgccacgg tggtcatcgt cgaccgcggg
     3301 ccgttcatcc gcagccggat catcgatgtt tcaacgcgcg ccgccgagat tctcggcttc
     3361 cggcgagcgg gcgtcgcccg cgtccggctg gaagtcgtca acaaataggc gcgcctagtt
     3421 cgcctcgtcg agcaggcgac gggcgatgac ctgcgcctgg atttccgccg cgccctcgaa
     3481 aatgttgagg acgcgggcat cgcaaagaac gcgcgagatc gggtattcga gcgcgaatcc
     3541 attgccgccg tgaatctgca aagcgttgtc cgccgcggcc caggcgacgc gggcggcgag
     3601 gagcttggcc atgcccgctt cgacatcgca gcgccggctt tcgtccttgg cgcgcgccgc
     3661 gaaataggcg atctggcggg cgatgtggat ttcgacggcc atcatgacga gcttgtcggc
     3721 gacgcgcgga aaggcgatca gcggcttgcc gaattgcctg cgctccttgg cgtaggcgag
     3781 gcccaattcc agcgccgatt gggcgacgcc gaccgcgcgc gccgcggtct ggatgcgggc
     3841 ggattcgaag gtcgccatca attgcttgaa acccagccct tcttggccgc cgagcaggcc
     3901 ggaggcgggg atttgaaagc cgtcaaaagc gatttcgaat tccttcatgc cgcgatagcc
     3961 gaggacttcg atctcgccgc ccgacatgcc gggcgccggg aacggctcgg cgtcgccgcc
     4021 gcgcggtttg cgcgcaagaa acatggaaag gccgcgatag cccttctcgc tcggatcggt
     4081 gcgcgccagc aaggtcatca gatcggcgcg gaccggatgg gtgatccagg tcttggcgcc
     4141 atggatgcgg tagacgtcgc cgtcgcggac ggcgcgcgtg gcgaggctcg caagatccga
     4201 gccggtattc ggttcggtga agaccgccgt cggcaggatt tcgcccgagg cgatcttggg
     4261 caggaattcg cgcttctgcg cctcggtccc gccggacagg atcagttcgg cggcaatttc
     4321 ggagcgcgtg ccgagcgacc cgacgccgat atagccgcgc gacaattcct cggtgacgac
     4381 gcacatcgcc actttgccga ggcccatgcc gccataggct tccgggatgg tcaggccgaa
     4441 cacgcccata ttcgcgaggc gtgcgatcac gtcgagcgga atataggcgt tggcgcggtg
     4501 ccaggcctgc gcgtgcggag cgatctccgc ctccgcgaaa cgccgcatct cggcgcggat
     4561 cgcgtcgagc gtgtcgtcga gaccgggatc gccgaatgtc gtggaatggg agtcggcgag
     4621 gaggtcggcc aaggcgcggc gattggcggg attgttttcc tccatcaaag ccgccgccgc
     4681 gccgtcgcgc cgcgccgcga tctgccgtgc gccaaggccg aaatcgccga ggcgcacgat
     4741 ctcgccctgg ctcatcggaa tcccgccgaa aatctgagcg aggaattcgc cgaggccgat
     4801 cgccgtcagc agcgcctcgg ccgggccgaa gcgccgctcg cgccgcaggc ggcgggcgta
     4861 ggagactatc tcgcgcgccg cgctggcata ggtcgcgagc caggcgagcc catgcgcggc
     4921 gcgctgctcg cgctcgatca gggcggcgct gaggcggccg ccgtcggcga cccgcgcccg
     4981 gaccgtggcg acggcgtccc cgagcagcgc ttcgatcgag gcgacagcct ggttcacgcg
     5041 cgcctcgtcg aaggcgctcg tctcggcgct aagaccggtt gcggattcgg cggaaagggt
     5101 caggggcgct cccgagccga cggcatgatg ttgcgctgca gcatgcaccg tcgcggcggc
     5161 tcacgcaatc ccttgcgccg gcttcgaccg agcgatggct tttttcgcgc ggatcaggtt
     5221 tcccatttgc ccgcggcggg cgcatgctcc atacgcgaac cggcgac
//
LOCUS       DATGJW010000120         3157 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1266513, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000120 DATGJW010000000
VERSION     DATGJW010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3157)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3157)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3157
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1266513"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..645)
                     /locus_tag="VKU03_03500"
     CDS             complement(<1..645)
                     /locus_tag="VKU03_03500"
                     /inference="COORDINATES: protein motif:HMM:NF014628.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HLJ70356.1"
                     /translation="MTAARDQILGSIRRSLKRGPLGEAETAALRARLASPGRNLVPAR
                     AAALDPPAQRDLFTAMAQEVQTTVDRVASLAEVPAAVASYLAAENLPAELVMAPDPAL
                     DDIPWHERPLLQFRRGRAEPGDQVSLTPAFAGIAETGTLMLVSGAETPSTLNFLPDTH
                     IVVLRAEQVVPSLEEAWTKLRARQAARGDAALPRTVNFVTGPSRTADIEQRLLLG"
     gene            complement(662..2104)
                     /locus_tag="VKU03_03505"
     CDS             complement(662..2104)
                     /locus_tag="VKU03_03505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113791.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0019516 - lactate oxidation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LutB/LldF family L-lactate oxidation iron-sulfur
                     protein"
                     /protein_id="HLJ70357.1"
                     /translation="MTVQPTSSHFKENAHEALRDAPLQKALVHVRANFIQKRAEAARR
                     LPEFEALRDSARDIKKHTLQHLDLYLEAYEAKVRELGGEVHYAATAKQGVDIVLDICR
                     RRGAKSVTKGKSMISEEIGLNQALEAAGIEAVETDLGEYIIQLRGEAPSHIIAPAVHV
                     NKEEVEEEFRRAHLHLPKGRDLSQPAQLLGEARGVLRDKFLNADIGITGANFLVAETG
                     TSIIVTNEGNGDLTQILPKVHVVIASLEKITPTLEDAAQLFRVLARSATGQDMSVYTT
                     LSTGPCRPGDPDGPEEYHVVLIDNGRSSMLGGEFEDMLRCIRCGACMNHCPVYHAIGG
                     HAYGWVYPGPMGAVLTPTLIGVKEGGQLPNASTFCGRCEEVCPVKIPLPKMMRHWRER
                     EYEKKLSPAAFRAGLGLWAFFARRPELYHRMAAIGARILGGFGRRRGRFRALPLARGW
                     TSVRDMPAPQGRSFQSLWAERQRARMPRYH"
     gene            complement(2101..2901)
                     /locus_tag="VKU03_03510"
     CDS             complement(2101..2901)
                     /locus_tag="VKU03_03510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007196216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HLJ70358.1"
                     /translation="MKDNALADAARNPIRVGLFVTCLVDLFRPSVGFAAVKLLEAAGC
                     SVFAPSQTCCGQPAYNSGDRGASKALARQVIAAFDDCDYVVAPSGSCAGMLAKHYPEL
                     FADEPEWTAKARSFAAKCHELVSFLVDVLHVEKVAARFDGLVTYHDSCSGLRELGVQR
                     QPRQLLAGIEGLQLVEMKSPDVCCGFGGTFAVKYGELSDAIVAQKTANIAASGATMLL
                     AGDLGCLMNMAGKLQREGKPVAARHIAEVLAGMTDEPPIGEPKAGTRK"
BASE COUNT          503 a         1039 c         1091 g          524 t
ORIGIN      
        1 gccgagcagc aggcgctgct cgatatcggc ggtgcgcgac gggccggtga cgaaattgac
       61 ggtgcgcggc agcgccgcgt cgccgcgcgc cgcttggcgt gcgcgcagct tcgtccaggc
      121 ctcttcgagg ctcggcacga cctgctcggc gcgcagcacc acgatatgcg tgtcgggaag
      181 gaagttgaga gtcgacggcg tttcggcgcc ggagaccagc atcaaggtgc cggtctcggc
      241 gatgccggcg aaggccgggg tgaggctcac ctgatcgcct ggctcggccc ggccgcggcg
      301 gaactggagc aagggccgct cgtgccaggg aatgtcgtcg agcgccgggt ccggcgccat
      361 gacgagctcc gccggcaaat tctcggcggc gagataggac gccaccgccg caggcacctc
      421 ggcgagggag gcgacgcggt cgacagtggt ctgcacctcc tgcgccatgg cggtgaagag
      481 gtcgcgctgc gccggcgggt cgagcgcggc cgcccgcgcc ggaacgagat tgcggccggg
      541 cgaagcgagc cgcgcgcgca gcgccgccgt ttccgcctcg ccgagcggtc cgcgcttcag
      601 cgaccggcgg atgctgccga ggatctggtc gcgcgccgcc gtcattgtac gccgccccgc
      661 ctcaatggta gcgcggcatt cgggcgcgct gccgctccgc ccagaggctc tggaagctgc
      721 ggccttgcgg cgccggcatg tcgcgcaccg acgtccagcc ccgcgccagc ggcagggcgc
      781 ggaagcggcc gcggcggcgt ccgaagccgc cgaggatacg ggcgccgatc gccgccatgc
      841 ggtgatagag ctcgggacgg cgcgcgaaga atgcccagag gccgaggccg gcgcgaaatg
      901 ccgcgggcga taatttcttc tcgtattcgc gctcgcgcca atgccgcatc attttgggca
      961 gcgggatctt gaccgggcag acttcctcgc agcggccgca gaaggtcgag gcgttcggca
     1021 attgcccgcc ttccttcacc ccgatcaagg tcggcgtcag caccgcgccc atcgggccag
     1081 gatagaccca gccataagcg tgcccgccga tcgcatggta caccgggcaa tggttcatgc
     1141 aggcgccgca gcggatgcag cgcagcatgt cctcgaactc gccgcccagc atggacgagc
     1201 ggccattgtc gatgagcacg acgtggtact cttcggggcc gtccggatcg cccggccggc
     1261 acgggccggt cgacagcgtc gtatagaccg acatgtcctg gccggtcgcc gagcgggcga
     1321 ggacgcggaa aagctgcgcg gcgtcctcca aggtcggcgt aatcttctcc agcgacgcaa
     1381 tgacgacatg aaccttgggc aggatttgcg tcaggtcgcc attgccctca ttggtgacga
     1441 tgatcgacgt tcccgtctcg gcgacgagga aattcgcgcc ggtaatgccg atatcggcgt
     1501 tcaggaactt gtcgcgcagc acgccgcgcg cctcgccgag caattgcgcc ggctgcgaca
     1561 ggtctcgacc cttcggcaaa tggagatggg cgcggcggaa ctcctcctcg acctcctcct
     1621 tgttcacatg aaccgccggc gcgatgatgt gcgacggcgc ttcgccgcga agctggatga
     1681 tgtattcgcc gagatcggtc tcgaccgctt cgattccggc cgcttccaac gcctggttga
     1741 ggccgatctc ctcggaaatc atcgacttgc ccttggtgac gcttttcgcg ccgcgccttc
     1801 ggcagatgtc gagcacgatg tccacgccct gcttggcggt ggcggcgtaa tggacctcgc
     1861 cgccgagctc gcgcaccttg gcttcgtacg cttcgagata gagatcgaga tgctgaagcg
     1921 tatgcttctt gatgtcgcgc gcgctatcgc gcaaggcctc gaattcgggc agccgccgcg
     1981 ccgcctcggc gcgcttctgg atgaagttcg cgcgcacgtg gacgagcgcc ttttgcagcg
     2041 gcgcgtcgcg caaggcctcg tgcgcgtttt ccttgaaatg ggacgatgtc ggctgaacgg
     2101 tcatttccgg gtcccggctt tcggctcgcc gatcggcggc tcgtccgtca tgccagcgag
     2161 gacttcggcg atgtggcggg cggcgacggg cttgccctcg cgttgcagct tgccggccat
     2221 gttcatcagg cagccgagat cgccggcgag caacatcgtc gcgccgctcg cggcgatgtt
     2281 cgccgtcttc tgcgcgacga tggcgtcgga gagctcgcca tatttgacgg cgaacgtgcc
     2341 gccgaagccg cagcaaacgt ccggcgattt catctcgacg agctgcaagc cttcgattcc
     2401 ggcgagcaat tggcgcggct ggcgctgcac gccgagctcg cgcaggcccg agcaggaatc
     2461 gtgataggtg acgaggccgt cgaaccgcgc cgcgactttc tcgacatgga gcacatcgac
     2521 caggaagctg acgagctcgt ggcatttggc ggcgaaagag cgcgccttcg cggtccattc
     2581 cggctcgtcg gcgaaaagct cgggatagtg cttggcgagc atgccggcgc aggagccgct
     2641 cggcgcgacg acgtagtcgc agtcgtcgaa ggcggcgatc acctggcgcg ccagcgcctt
     2701 gctcgcgccg cggtcgccgg aattgtaggc cggctggccg cagcaggttt ggctcggcgc
     2761 gaagaccgag cagccggccg cctccagcag cttgaccgcg gcgaaaccga cgctcggacg
     2821 gaaaagatcg accagacagg tgacgaaaag cccgacgcga atcgggttgc gagcggcgtc
     2881 ggccaaggcg ttgtccttca ttttcctcga ttcgacattg gactagcgcg gccgtcgttt
     2941 caagcctgag atgtcattcc tgcggaagcg aaatccaggg gcgccgcgct ttgaggagag
     3001 gctgtactgg attccgcttg cgcgggaatg acatcgcgca atggtacgaa gggtttcgtc
     3061 cctcacgcca aatccgccag catgcccgac gcggccgagc ggagcagccg ctttcgcgcc
     3121 tcccattcgg ttgcgtcgtc caacgcggcc agcatct
//
LOCUS       DATGJW010000121        11849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1473582, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000121 DATGJW010000000
VERSION     DATGJW010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11849
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1473582"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..570)
                     /locus_tag="VKU03_03515"
     CDS             complement(7..570)
                     /locus_tag="VKU03_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024934.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLJ70359.1"
                     /translation="MTGLPVILRSMKTPLKAQVQIPGEALRISAEDLQSCLAASGALL
                     DLLLSYVQAMLVQSAYVGLCAAQHTARQRIRSWLLLSAAALGRSELSITHQAIARLLG
                     LRRATVSDCLADLQRSGAICQKRGGIEILDAETIAQEACECHRVIRAEFAHLWSSRPG
                     TARAAVANGAGSLSLFARERAENQADR"
     gene            complement(1156..1917)
                     /locus_tag="VKU03_03520"
     CDS             complement(1156..1917)
                     /locus_tag="VKU03_03520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ70360.1"
                     /translation="MNEARAAAAPKPVLSLKDIHKTFGGLTAIERFNLDVFAGEVVAL
                     VGDNGAGKSTLVKIIAGVHRPTSGRVLIDGAEAILTDPSDSQQRGIQVVYQDLALADS
                     QPVYMNMFLGRELVKGPLRRLDRKRMISETEALVKALDIRIPSARATIRELSGGQRQG
                     VAIARATHWAAKLVLMDEPTAALGVAETAKVESIISGLKARQLPILIVSHSLDQVFRV
                     ADRICVLRRGRQIGVREARRTDKNEIVSMITGLSA"
     gene            complement(1925..3115)
                     /locus_tag="VKU03_03525"
     CDS             complement(1925..3115)
                     /locus_tag="VKU03_03525"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594102.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLJ70361.1"
                     /translation="MFLDLIRRRNPKLIEQAIALHQAGRLPANSYVIDVDAVEENARV
                     IASAAGKLGLKVFAMTKQMGRNPSFCQAAARGGIVSAVAVDMEDARATCRAGLALGHI
                     GHLVQIPKFEADAAAALSPDYWTVFNSEKAAEAAAASHRRGRVQPLLARIQAEGDRFY
                     RGHEGGFPAADILRVADAFDALPGGRFAGITSFPALLFDPATRSVKTTPNLETLRRAR
                     EALAKAGRKDIEVNAPGTTSAKVLAFLAEAGATQVEPGHGLTGTTPLHVVEDLPERPA
                     VVYLTEVSHFAGGEAYCFGGGLYIDPVFPDYDVKAIVSSEPTASAAALKSVEIPSPAA
                     IDYYGMIKGGASAARVGDSVVFGFRPQAFVTRAYVVGLSGLARGAPHVEGVHDAFGRA
                     VAWP"
     gene            complement(3131..4075)
                     /locus_tag="VKU03_03530"
     CDS             complement(3131..4075)
                     /locus_tag="VKU03_03530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70362.1"
                     /translation="MFATLKSLSPSRYVIYVGFLAIFAAFSVILRNDGFATPTNLLNI
                     IQQTTPVTVMAVGAVFVLTAGEIDLSLGSVVAVSALLAATVLRNWAWPAGVAAGLGAG
                     ALIGALNGALVAYGRLPSFLVTLATMGVFAGVARRMTDLKSVPIENDAFNALFGSGAL
                     FGLPSLIVWTIVAVAVGYFVFRETRFGAHVHAVGDSANAARATGIKVLRLRFMVMLLS
                     GVLAALAGLLYAGRLNGARYTLGEADLLTVIAAVIVGGTKLNGGVGSIIGALIGSLLM
                     GMLNNGLILMGFQPSDQMIARGLIILIAVALTLREPNR"
     gene            complement(4224..5303)
                     /locus_tag="VKU03_03535"
     CDS             complement(4224..5303)
                     /locus_tag="VKU03_03535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008594110.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ70363.1"
                     /translation="MRTINRLGGMLLAGAIAGLIAANGAVAYAGETIGPDGEKATPSS
                     ALTLSDSEVAKIKSGKYTAALLWHTSSDFVNAVTAGATDEFNRLGMSIVAQTDAAFDA
                     AKQKSDVETVLAKKPSVILALPLDPVTSAEAFKPAVASGVKIVLLSNKPKGFVQGKDY
                     VTIVTDDLFQMGKHAADALAAALGKKGKIAWIYHDAQYYVTNQRDNAFKATIEADYPD
                     IKIVAQQGITDPARAQDIAQALLLKNPDLDGIYVTWAEPAEGVLAALRAAGNKHTKIV
                     TLDLSEPIGLDMVKGGNVVAIVADKAYELGRAMATAAGYGLIDKPAPPFVVAPVLTVT
                     KDNIVQGWKESLHREPPKSILDALK"
     gene            complement(5409..6653)
                     /locus_tag="VKU03_03540"
     CDS             complement(5409..6653)
                     /locus_tag="VKU03_03540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007010306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLJ70364.1"
                     /translation="MSDDRLIARLRDAFDAEAALALLRRAIGTPSITGNEAAFAAMLA
                     DELKALGAEDVALWDFAPGRPNVSALRKGRPGGDTVLLIGHTDTVHVRGWSEHWAGTE
                     RESPYSAALIDDAVWGRGASDLKAGICTILSALRTLDRAGVALDPTVLFAFVGDEESG
                     EEGSGVSAGAHALANRIEKGELPKPNFAIYVEPTMLDVYVAHMGFFLCDITMTGKSAY
                     FGVPELGVDALRAANQALSALWRYSDELARGEEHPLVGAGFVLPTGIQGGGYIAVPGE
                     CKISLIAKIPPGVDLETVRRGVEAAVRAAIVDERVKVDFAYPAGRDHPIGGRPFESPP
                     DDGRVDRLVASVRAIRPDRGRIEAAPYWSELPFFAALGVPGVYFAPGDITICHTVGEN
                     VNLKDYNDGILALASYLARRDP"
     gene            complement(6650..7873)
                     /locus_tag="VKU03_03545"
     CDS             complement(6650..7873)
                     /locus_tag="VKU03_03545"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HLJ70365.1"
                     /translation="MLQQTTQPRVTRQLSQRAVFEGLLHKGPISRADLAKLTGLSKQT
                     ISEVVDDFEQRGWARPVGRTSGNIGRTAVLYELCPDSGYVVGVDLGGTKVTAAIADMS
                     CATLLEITERTDPRGGRAVLDQIARLAAKLARDINAHPSRVLCAAIGTPGVVNKRTGV
                     IELAPNVPGFDELDVGAILKERLGGHVLIENDVNLALLGEIWQGRARDAAHVAFLAFG
                     TGVGLGLAANGRLVRGENGAAGEIGYLPVGGDAFAPETRNQGALEYEIGAAGFMRRYR
                     AAGGAAADSVKEIFDRLAAGEPTAAKVIDETARIAALAAASVAALFDPQLIVVGGSIG
                     GRAEFLDRLQAHAALCAPRPLEICASALQNKAGVVGALAVALNWLHEDLFGLPDLPGE
                     LPLPAPKALGARAAR"
     gene            8042..8623
                     /locus_tag="VKU03_03550"
     CDS             8042..8623
                     /locus_tag="VKU03_03550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase"
                     /protein_id="HLJ70366.1"
                     /translation="MRILLLYAHPVETGFLGTLHRRVAETLRQRQHQVDDCDLYAERF
                     NPIMSRETYLHYLDTTANRAQARPYIDRLLAAEALVLISPIWHDGFPAILKGFFDCVF
                     VPGVAFTLQEGRFAPALRNIKRVAAICSYGAERERTVAMGDPQRRFVKRALRDQIGPG
                     GRSDFIGLFGMDAATEQTRARYLKRVTRAFSFW"
     gene            complement(8636..8935)
                     /locus_tag="VKU03_03555"
     CDS             complement(8636..8935)
                     /locus_tag="VKU03_03555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70367.1"
                     /translation="MASADQPKVAIYRQGVDAPKFEHVWCRWGCRHGWGWRGPALLGG
                     LAAGAIIGGALAAPYYGSGPYYGGSYYGGPYGGPYYPGCWRSVWTAYGWRRAWVC"
     gene            complement(9216..9872)
                     /locus_tag="VKU03_03560"
     CDS             complement(9216..9872)
                     /locus_tag="VKU03_03560"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008379 - thioredoxin peroxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLJ70368.1"
                     /translation="MLQIGQVAPDFTAQTTEGPIRFHDWIGNSWAVLFSHPKDFTPVC
                     TTELGAVARLKGEFEKRGAKVIGLSVDPVSSHEKWAKDIEETQGVAPNFPMIGDTDLS
                     ISKTYGMLPASAPNSSEGRTPNDNATVRTVFVIAPDKTIKLQLAYPMSTGRNFAEILR
                     ALDSLQLTSKFSVSTPADWKPGEDVIISGSVSNEDAAKRFPNGWKTPKPYMRIVAQPR
                     "
     gene            10110..10397
                     /locus_tag="VKU03_03565"
     CDS             10110..10397
                     /locus_tag="VKU03_03565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ70369.1"
                     /translation="MITSGQMRAARALLDIDQKHLAELSGVSLPTIQRMEASDGVVRC
                     VVDTLIKVVEALDRAGVALIGDNAASNSGGRGVRLKTPPASTAATQNASAQ"
     gene            10394..>11849
                     /locus_tag="VKU03_03570"
     CDS             10394..>11849
                     /locus_tag="VKU03_03570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015116 - sulfate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SulP family inorganic anion transporter"
                     /protein_id="HLJ70370.1"
                     /translation="MTAQLRQPSFSELYTPKLIVALRERYGLVAFKADLLAGLTVAIV
                     ALPLSMAIAIASGVTPDRGLYTSIFGGFVVSVLGGSRFQIGGPAGAFIVLVAAAVARH
                     GVDGVILATFLAGMILLAVGFLRLGAFIKYIPYPVMVGFTSGIAVIIFASQVKDLLGL
                     NLPSGEPGPILPKARALAAALPTANWAAIAVAASAIVVMVVIRKYRPHWPAYLIAVGL
                     GAVATALLGLPIETIGSRFGGIPQAIPAPRLPALSLGKIVEMLPTAFSFALLAGIESL
                     LSAVVADSMTGRRHRSNGEIVAQGAANIASALFGGICTTGLIARTATNVRAGARSPIA
                     GMLHSIFLLLFMLLAAPLARYIPLAALAAVLAVVAWNMAEKHEFLTLLRASRGDAVVL
                     LATFALVVFRDLTEGILVGFGIGALLFLHRMAQTARVELPEPAGGDEAPFLATDPDIV
                     AYRISGAFFFGSAASVAMALDRIGEHPKAYVIDFS"
BASE COUNT         2054 a         3915 c         3776 g         2104 t
ORIGIN      
        1 ctaattttat cggtcggcct gattctccgc ccgctcccgc gcgaagagac taagcgaccc
       61 ggccccgttc gcgaccgcgg cgcgggcggt ccccggtcgg ctactccaca aatgggcaaa
      121 ttcggcgcgg atgacgcggt ggcattcgca agcctcctgc gcgatcgttt cagcgtccaa
      181 aatctcgatg ccgccgcgtt tctggcaaat cgctcccgat ctctgaaggt cggccagaca
      241 atcgctgacc gtcgcgcggc gcaggcccag cagcctggca atggcctgat gggtgatcga
      301 aagctcgctt cggccaaggg cggcggcgct cagcagcagc caactgcgaa tccgttggcg
      361 ggccgtatgc tgggcagcgc acaatccgac ataggcgctt tgcaccagca ttgcctgaac
      421 ataggaaagc agaaggtcaa gcaatgcgcc cgaagccgcc aggcacgatt gcaaatcctc
      481 ggcggaaatc cgcaacgctt ccccgggaat ctggacctga gccttgagcg gcgtcttcat
      541 tgaccggaga atcacaggca agccggtcat gccttccgat ccgatcaatc cggaagcgat
      601 caggccggcg cctctgctga ccgcctgcag ggacgattcc cgactcgatg aaataggcga
      661 agctgaccgg catgcgcggc tgttcgagaa cctggcctct cttcagcttt acgcgctttg
      721 ctttcgccat gacgagattg aagctgtcag gcggaagctc caacagaagc cggttgacgg
      781 cggtcggctg cgcgatcggc gggacgatga ttttggcgcc ggtcggcgcg tctctgcgct
      841 ccgtttccgg gtggcggcga ggcgagtcca gaaagatccg cggcggacgc cgcgcttcaa
      901 tgacgtcgct gacctgatga aggacgctcc aggaatcgtc gaccgccgtc cgcgtttggg
      961 cgaccgccgc gcgacaatcc gcggcaagac ccatcagtcg cgcagtatct gaccaaattc
     1021 ttcgagcatc ggccgcaatc ccgatcggcg ccaggtaaat agcggctcgc gcggccgcct
     1081 gccgccgcgc cgctcaggat ggcgtttatc gccgcatcag gcaattccaa attttcggaa
     1141 ggcttcgccg gccgctcagg cgctgagtcc ggtgatcatc gaaacgatct cgttcttatc
     1201 cgtgcgcctg gcctcgcgaa cgccgatctg gcgcccccgt ctcaacacgc agatgcggtc
     1261 cgcgacgcgg aagacctgat ccagactgtg actgacgatc aggatcggca attgccgcgc
     1321 cttcaggccg gaaatgatcg actccacctt ggcggtttcg gcgacgccga gcgcggcggt
     1381 gggctcgtcc atcagcacga gcttggccgc ccaatgcgtg gcgcgcgcga tggcgacgcc
     1441 ctggcgctgg ccgccggaca gctcccggat cgtcgcgcgg gcggagggga tgcggatgtc
     1501 aagcgctttg accagcgctt ccgtctcgct gatcattcgc ttgcggtcga gacgccgcag
     1561 cggccctttc acgagttcgc ggccgagaaa catattcata tagaccggct ggctgtcggc
     1621 gagggccaag tcttggtaca cgacctggat gccgcgctgc tgcgagtcgc tcggatcggt
     1681 caagatcgct tcggcgccgt cgatcagaac gcggccggag gtcggccggt gcacgccggc
     1741 gatgatcttg acgagcgtcg acttgccggc gccgttatcg ccgaccaacg ccaccacctc
     1801 gccggcgaaa acatcgagat tgaaccgctc gatcgcggtc aggccgccga acgtcttgtg
     1861 aatgtccttc aaggaaagga ccggtttcgg cgcggctgcc gcgcgagctt cgttcatggc
     1921 gcgctcacgg ccaggcgaca gcgcggccaa acgcgtcatg tacgccttcg acatgcggcg
     1981 cgccgcgcgc caggcccgac aggccgacga cataggcgcg cgtgacgaaa gcctgcggcc
     2041 ggaagccgaa cacgacgctg tcgccgacgc gcgccgccga cgcgcctcct ttgatcatgc
     2101 cgtaatagtc gatcgcggcg ggagacggaa tctccaccga tttcagcgcg gcggctgagg
     2161 cggtcggctc ggacgagacg atcgctttca cgtcgtaatc gggaaagacg ggatcgatat
     2221 agagcccgcc gccgaagcaa taggcttcgc ctccggcgaa atgagaaact tcggtcagat
     2281 agacgaccgc gggccgctcc ggcaggtctt cgacgacgtg aagcggcgtc gtgccggtca
     2341 gcccgtgccc cggctcgact tgcgtcgccc ccgcttcagc gaggaaagca agaacctttg
     2401 ccgaggtcgt gccgggcgcg ttgacttcga tgtctttgcg gccggccttg gccaaagcct
     2461 ctctcgcgcg gcgcagcgtt tcgaggttcg gcgtcgtctt gaccgaccgc gtcgccggat
     2521 cgaagaggag cgccggaaag gacgtgatcc cggcgaagcg gccgccggga agcgcgtcga
     2581 acgcgtcggc gacgcgcaag atgtcggcgg cgggaaatcc gccctcgtgc ccgcgataga
     2641 agcggtcgcc ttccgcctga atgcgggcga gcagcggctg aacgcggccg cgccgatgcg
     2701 aggccgcggc ggcttcggcg gctttttcgg aattgaacac tgtccagtag tccggcgaca
     2761 gcgccgccgc ggcgtccgct tcgaatttcg gaatctgcac cagatggccg atatggccga
     2821 gggcgagacc ggcgcgacac gtcgcgcggg cgtcctccat gtcgaccgcg accgcgctga
     2881 cgattccgcc gcgcgccgcc gcttggcaga aggacggatt gcgccccatc tgcttggtca
     2941 tcgcaaagac tttcaggccg agcttgccgg cggcgctggc gatgacccgc gcattctctt
     3001 cgacggcgtc gacatcgatc acataggaat tggcgggcag ccggccggct tgatgcaggg
     3061 cgatggcctg ctcgatcagc ttcgggttgc ggcggcggat caggtcgagg aacatgtctc
     3121 gaagctccaa tcagcggttg ggttcgcgca aagtcagcgc cacggcgatg aggatgatga
     3181 ggccgcgcgc gatcatctgg tcggatggct ggaagcccat cagaatcagg ccgttattca
     3241 gcatccccat gagcagcgag ccgatcagcg cgccgatgat cgagccgacg ccgccattga
     3301 gcttggtgcc gccgacgatc accgcggcga tgaccgtcaa gagatcggct tcgccgagcg
     3361 tatagcgcgc gccgttcagc cggccggcat agaggaggcc ggccaaagcg gcgagcacgc
     3421 cgctcagcag catcaccatg aagcgcagcc tcagcacctt gatgccggtg gcgcgcgccg
     3481 cgttggcgct gtcgccgacc gcatggacat gggcgccgaa ccgcgtctcg cgaaagacga
     3541 aatagccgac cgcaacggcg acgatggtcc agacgatcag cgacggaagg ccgaacagcg
     3601 cgccggagcc gaacaaagcg ttgaacgcgt cattctcgat cggcacggat ttgagatcgg
     3661 tcatccgccg cgcgacgccg gcgaaaacgc ccattgtcgc caaggtgacg agaaacgacg
     3721 gcaagcggcc ataggcgacc agcgcgccgt tgagcgcgcc gatcagcgcc ccggccccga
     3781 gccccgccgc gacgccggcc ggccaagccc agttgcgcag gaccgtcgcg gcgagcagcg
     3841 ccgagaccgc gacgaccgag ccgagcgaca gatcgatctc gccggcggtg agaacgaaca
     3901 cggcgccgac cgccatgacc gtaaccggcg tcgtctgctg gatgatgttg agtagattgg
     3961 tcggggtggc gaatccgtca ttgcgcagaa tgacggagaa agccgcgaaa atcgccaaga
     4021 aaccgacata gatgacgtac cggcttggcg acagagactt cagcgtggcg aacatggctg
     4081 cgcccgatcg gtcgccggcg cgcgccgagc gcgccggttg ggcgccgtcg cgccgttcgg
     4141 atgagcgcaa ccaagcgctt ttcatcttgc tgcgctcgcg ccggcggagg gcgcctgggc
     4201 cgatccgccg gctttgaccg atgtcacttg agcgcgtcga ggatggattt cggcggctcg
     4261 cgatgcagcg attctttcca accttgcacg atattgtctt tggtcacagt gaggaccggc
     4321 gcgacgacga aaggcggcgc gggcttgtcg atcaggccgt agcccgccgc ggtcgccatg
     4381 gcgcggccga gctcgtaggc cttatcggcg acgatcgcga cgacattgcc gcccttgacc
     4441 atgtcgaggc cgatcggctc ggagagatcg agcgtgacga tcttcgtgtg cttgttgccg
     4501 gcggcccgca aagcggcgag cacgccttca gcaggctcgg cccaggtcac atagattccg
     4561 tcgaggtccg gattcttcag cagcagcgcc tgggcgatgt cctgcgcgcg ggcggggtcg
     4621 gtaatcccct gctgcgcgac gatcttgatg tcgggatagt ccgcttcgat cgtcgccttg
     4681 aaggcgttgt cgcgctggtt ggtcacgtaa tattgcgcat cgtgatagat ccaggcgatc
     4741 ttgcctttct tgcccagagc ggcggcgagc gcgtcggccg cgtgcttgcc catttggaag
     4801 agatcgtcgg tgacgatcgt cacatagtcc ttgccttgta cgaaaccttt cggcttgttc
     4861 gatagcagga cgatcttgac gcccgaggcg acggccggct tgaacgcttc cgccgaggtg
     4921 acgggatcga gcggcagcgc cagaatgacg ctcggcttct tggcgaggac ggtctcgacg
     4981 tcgctcttct gcttggccgc gtcgaaggcg gcgtcggttt gcgcgacgat gctcatgccg
     5041 agccggttga actcgtccgt cgcgccggcc gtgactgcgt tcacgaaatc cgaggaggtg
     5101 tgccacaaca aggcggccgt atatttgccg ctcttgatct tcgcgacttc gctgtcgctc
     5161 aatgtcagcg cgcttgacgg cgtcgccttc tcgccgtccg gcccgatcgt ctcgccggca
     5221 taggcgacgg cgccattcgc cgcgatgaga ccggcgatcg cgccagccag cagcatcccg
     5281 cccagcctgt ttattgtcct catggctcgc tccctctcca ctgctttcgc agttctcctc
     5341 gccgcatctg attagttcgt ataattgacg aattaaatgc cgcgtaccag tccgtgtcaa
     5401 gagcggtctc aggggtcgcg acgcgccagg taagaagcca gcgcgaggat tccgtcatta
     5461 taatccttga gattcacgtt ctcgccgacg gtatggcaga tggtgatgtc gccgggggcg
     5521 aaatagacgc cgggaacgcc caaagcggcg aagaagggca gctccgacca gtagggcgcc
     5581 gcctcgatcc ggccgcggtc cgggcgaatg gcgcgcacgc tggcgaccag ccgatcgacg
     5641 cggccgtcat ccggcgggct ttcgaagggc cggccgccga tcgggtggtc ccgccctgcc
     5701 ggataggcga aatcgacttt gacgcgctca tcgacgatcg ccgcccgtac cgctgcttcg
     5761 acgccgcgcc gtactgtctc gagatcgacg ccgggcggaa tcttggcgat caggctgatc
     5821 ttacattcgc ccggcacggc gatgtagccg ccgccttgaa tgccggtcgg caggacgaag
     5881 ccagcgccga ccaacggatg ctcttcgccg cgcgcgagtt cgtcggaata gcgccacaac
     5941 gcggaaagag cttgattggc cgctctcagc gcgtcgacgc cgagctccgg cacgccgaaa
     6001 taggccgatt tgccggtcat cgtgatatcg cacagaaaga agcccatatg cgcaacgtag
     6061 acatcgagca tggtcggctc gacataaatc gcgaaattcg gcttgggcaa ttcgcctttt
     6121 tcgatccggt tcgcgagagc gtgcgctccg gcgctgacgc cgctcccctc ttcgccgctt
     6181 tcttcgtcgc cgacgaaggc gaagagtaca gtcggatcga gcgcaacccc ggcccggtcc
     6241 aatgtgcgca acgcggagag aatggtgcag atcccggctt tgagatcgct cgcgccgcgt
     6301 ccccagaccg cgtcgtcgat cagcgccgcc gaataagggc tctcgcgctc cgtaccggcc
     6361 caatgctcgc tccagccgcg cacatgcacg gtgtcggtat ggccgatcag cagcaccgtg
     6421 tcgccgccgg ggcgcccctt acgcaaggcc gaaacgttcg gccggcccgg cgcgaaatcc
     6481 cacaaggcga cgtcctcggc gccaagcgcc ttcaattcgt cggcgagcat cgcggcgaac
     6541 gccgcctcat tgccggtaat gctcggcgtt ccgatcgccc gcctcagcaa agcaagcgcc
     6601 gcctccgcgt caaaagcgtc gcgcagccgc gcgatcaatc gatcgtcgct catcgcgcag
     6661 cccgcgcgcc gagcgccttc ggcgccggca gcggcaattc gcccggcaga tcggggaggc
     6721 cgaacaggtc ctcgtgcagc cagttcagcg ccacagcgag cgcgccgacc acgccggctt
     6781 tgttctgaag cgcgctggcg cagatttcga gcggccgcgg cgcgcaaagc gcggcatgcg
     6841 cctggaggcg gtcgaggaat tccgcccgcc cgccgatcga gccgccgacg acgatgagct
     6901 gcggatcgaa cagagccgcg acggaagcgg cggcgagcgc cgcgatccgg gcggtttcgt
     6961 cgatcacttt cgccgccgtc ggctcgccgg cggcaaggcg gtcgaaaatc tccttgacgc
     7021 tgtcggctgc cgcgccgccg gcggcgcggt agcgacgcat gaatccggcg gcgccgatct
     7081 catattccag cgcgccctga ttgcgggtct ccggcgcgaa agcgtcgccg ccgaccggca
     7141 gatagccgat ctccccggca gcgccgttct cgccgcggac gagccgcccg ttcgccgcca
     7201 ggccgaggcc gacgccggtg ccgaatgcga ggaacgcgac atgggcggcg tcgcgcgccc
     7261 gcccctgcca gatttcgccg agcagcgcga gattgacgtc gttctcgatc aggacgtggc
     7321 cgccgagcct ttccttcaag atcgcgccga cgtccaattc gtcgaaaccg ggaacgttcg
     7381 gcgcaagctc gatcacgccg gttcgcttgt tcacgacgcc aggcgtgccg atcgccgcgc
     7441 aaagcacgcg cgaaggatgc gcgttgatgt ctcgcgccag cttggccgcg agccgggcga
     7501 tctggtcgag aacggcgcgc ccgccgcgcg gatcggtccg ctcggtgatc tccagcagcg
     7561 ttgcacagga catgtcggcg atcgccgccg tgaccttggt gccgccaaga tcgacgccga
     7621 cgacatagcc ggaatcagga cagagctcgt aaagcaccgc cgtccggcca atattgccgc
     7681 tggtccggcc gaccggccgc gcccagccgc gctgctcaaa atcgtcgacg acttcggaaa
     7741 tggtctgctt ggagagtccg gtgagcttgg cgagatcggc ccgcgagatc ggccccttat
     7801 ggagcaagcc ctcgaaaacg gcgcgctgcg agagctgccg ggtcacccgg ggttgcgtgg
     7861 tttgttgcag catcgggccc gcgtattagt tcggtttgta gccgaattat atgcggatgg
     7921 cctggagccg ctgtcaaccg gcggcgccgc gaccggcctg gagaacgcgg gactatcgaa
     7981 tcacgccggt ttcggcgtag cggggtctaa gcgaagcccg gcgcgaggaa aaagaacatc
     8041 aatgcggatc ctccttctct atgcgcatcc ggtcgagacc ggctttctcg ggacgcttca
     8101 ccgccgtgtc gccgaaacgc tgcgccagcg ccaacatcag gtcgacgatt gcgatcttta
     8161 tgcggagcgc ttcaatccca tcatgtcgcg cgagacctac cttcattatc tcgacacgac
     8221 cgccaatcgg gcgcaggcgc gcccctacat cgatcgcctg ctcgccgccg aagcgctcgt
     8281 actcatctcg ccgatctggc atgacggctt tccggcgatt ttgaagggat ttttcgactg
     8341 cgtcttcgtt cccggcgtcg ctttcacgct gcaggagggc cggttcgcgc cggccttgcg
     8401 caacatcaag cgcgtcgccg ccatctgctc ctacggcgcc gaacgcgagc gaaccgtcgc
     8461 catgggcgat ccgcagcggc gcttcgtcaa gcgcgccttg cgcgatcaga tcggtcccgg
     8521 cgggcgcagc gactttatcg gtcttttcgg catggacgcg gccaccgagc aaacgcgcgc
     8581 gcgctatctc aagagagtga cgcgcgcgtt cagcttttgg tgaaggcttc gccgcctaac
     8641 agacccaagc gcgccgccag ccataggcgg tccaaaccga gcgccagcag cccggataat
     8701 agggtccgcc atatgggccg ccataatagg agccgccata gtacgggcca gagccgtaat
     8761 agggcgcggc caaggcgccg ccgatgattg cgcccgccgc gaggccgccc agcagcgccg
     8821 gcccgcgcca gccccagcca tggcggcagc cccagcggca ccagacatgt tcgaacttcg
     8881 gcgcgtcaac gccttgccga tagatcgcga ccttcggctg atcggcgctc gccattggcg
     8941 ccatgtcggc aaaggtgaaa gcgccggctg gtccgcaggc cgtcaggagc gctgacgcgg
     9001 ctgcggcgac tttggctagg cgatgtcgaa gagcgaagag acccatagcg ccgtcctccc
     9061 tcgatcggag cgatcgcgaa ttctacggcg cgtgatcggg agaagcaatc ggaggctaca
     9121 gcccatgtcc cagccggatt tgtcccggac aggacgatcg tcgcagcggc gcgtcacatt
     9181 gcgccggctt cagcttctcg gacgccctcg cccgcttagc gcggctgcgc gacgatccgc
     9241 atatagggct tcggcgtctt ccagccgttc gggaagcgct tagcggcgtc ctcattgctg
     9301 accgaaccgg aaatgatcac atcctcgccc ggtttccaat cggccggcgt cgagaccgag
     9361 aatttcgacg tcagttgcag cgaatccaag gcgcgcagga tttccgcgaa gttgcgccca
     9421 gtagacatcg gataggcgag ctgcagcttg atcgtcttgt ctggcgcgat gacgaagacg
     9481 gtacggactg tggcgttgtc gttcggcgtt cggccttccg acgaattcgg cgccgaggcc
     9541 ggcagcatgc cataggtctt cgagatcgaa aggtcggtat cgccgatcat cggaaagttc
     9601 ggcgcgacgc cctgcgtctc ctcgatgtcc ttggcccatt tctcgtgcga gctcaccgga
     9661 tcgaccgata ggccgatcac tttggcgccg cgcttctcga attcgccttt gagtctggcg
     9721 accgcgccaa gctcggtcgt gcagacgggc gtgaaatcct tcggatgcga aaacagcacg
     9781 gcccacgaat tgccgatcca atcatggaag cggatcgggc cttcggttgt ctgggcggtg
     9841 aaatcgggag cgacttgtcc aatctgcaac atggctaact ccattttgat gacggcgcga
     9901 tggcgtccgc ggcgctgtcc ggcttgttgc cctgaagcta ctcgcctgga cgcttattat
     9961 caatccatta atgttataga ttttatttaa cagatgagta atgagataca caagattgtg
    10021 aagttaatgg taaatagtgc tatatagctt ctatttacgt ccagtacgat atataataca
    10081 tcagtcccga cattcgtgag caaatttcca tgatcacctc cggccagatg cgggccgccc
    10141 gcgccctcct cgacatcgac caaaaacatt tggccgaact ctctggcgtt tcgttgccga
    10201 cgatccaacg catggaggcc agcgatggcg tcgtacgttg cgtggttgac acgttgatca
    10261 aagtcgtcga ggcgctcgat cgagcgggcg tcgcgctgat cggcgacaac gccgcgagca
    10321 acagcggcgg acgcggcgtc cgcctcaaaa cgcctccggc ttcgaccgcc gccacgcaga
    10381 acgcgagcgc gcaatgaccg cgcaactccg ccaaccgagc ttttccgaac tctatacgcc
    10441 caagctgatc gtcgcgctgc gtgagcgcta cggcctggtc gcgttcaagg ccgatctcct
    10501 tgcaggcctc accgtcgcca tcgtcgccct gccgctttca atggccatcg ccatcgcctc
    10561 cggcgtaacg cccgaccgcg gcctttacac atcgatattc ggcggcttcg tcgtgtctgt
    10621 tctgggcggg agccgatttc agatcggcgg ccccgccggc gccttcatcg tcctggtcgc
    10681 cgcggcggtc gcgcggcatg gcgttgacgg ggtcattctg gcgacctttc tcgccggcat
    10741 gattttgctc gccgtcggat ttcttcgcct cggcgcgttc atcaaataca ttccgtaccc
    10801 ggtcatggtc ggcttcacgt ccggcatcgc cgtcatcatc ttcgcaagcc aggtcaaaga
    10861 ccttctcggc ttgaatctcc caagcggcga gcctggaccg atcctgccga aagcgcgcgc
    10921 cctcgccgca gcgttgccaa cggccaattg ggcggcgatc gccgtcgcgg cgagcgcgat
    10981 cgtcgtgatg gtcgtcatcc ggaaatatcg gccgcactgg cccgcttatt tgatcgcggt
    11041 cgggctcggc gcggtcgcaa ccgcactgct cggcctgccg atcgagacga tcggctcgcg
    11101 gtttggcggc attcctcaag cgattccggc gccgcgccta ccggcgctct cgctcgggaa
    11161 aatcgtcgaa atgctcccca ccgccttctc cttcgcgctg ctggccggca tcgagagcct
    11221 cctctcggcg gtcgtcgccg acagcatgac cggacggcgc catcgctcga acggcgagat
    11281 cgtcgcccaa ggcgccgcca acatcgcctc ggccttgttt ggcgggattt gcacgacagg
    11341 cctcatcgcc cgcaccgcga ccaatgtgag agccggcgcg cgctcgccca tcgccggcat
    11401 gctgcattcg attttcttgc tccttttcat gctcctggcc gcgccgctgg cgcgctacat
    11461 cccgcttgcc gcgcttgccg ccgttcttgc cgtggtcgcc tggaacatgg ccgagaaaca
    11521 cgaattcctg actctgctcc gggcgtcgcg cggcgacgcc gtcgtccttc tcgcgacgtt
    11581 cgcgctggtc gtctttcgcg atctcaccga aggcattctc gtcggtttcg gcattggcgc
    11641 ccttctcttt ctgcatcgca tggcgcaaac ggcgagggtc gaattgcctg agccggcagg
    11701 cggcgacgaa gcgcccttcc tcgcgaccga tccggacatc gtcgcctatc gcatatccgg
    11761 cgccttcttc ttcggctcgg cggcgagcgt ggcgatggcg ctcgaccgca tcggcgaaca
    11821 tcccaaagcc tatgtgatcg atttctccg
//
LOCUS       DATGJW010000122         6073 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_541384, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000122 DATGJW010000000
VERSION     DATGJW010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6073)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6073)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6073
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_541384"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VKU03_03575"
     CDS             complement(<1..1317)
                     /locus_tag="VKU03_03575"
                     /inference="COORDINATES: protein motif:HMM:NF013015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase Y family protein"
                     /protein_id="HLJ70371.1"
                     /translation="MIRRYLSLWLPRLSTDRARALLRRERGASPSPAPLATYAKIKNA
                     QRLVCVDRLAASLGLAVGLPLADAKARRPALVVVEANPLEEGKLLARIADWLQRFTPL
                     VALDGADGIMLDIAGVAHLFGGEEALARDIETRLAGQGLAARAGIADFPRAAWALARF
                     SRQRLAPAGVAGKSFAQLYHGLPLAALGLSESVAADMARAGLRQIGDIAMRPRAPVAA
                     RFGEDVIARFDALCGLERGAMSPRFAAPDFAAERRFASAIQERAAIEAALFKLAENLA
                     ALLQRQAKGARRLELALFRVDGATRRIAVGASRPLNEAGAIARLFREKFEAPEESEFD
                     SGYGFDVLRLSCLLAEPLAPAQGDFARPPHEEDRRALADLLDRLSARLGARRVTRIEC
                     VETHIPEFAVMAIPATLGEARTPSPARRQAPSTALRAVPLPRCAGED"
     gene            complement(1244..2098)
                     /locus_tag="VKU03_03580"
     CDS             complement(1244..2098)
                     /locus_tag="VKU03_03580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70372.1"
                     /translation="MPPILSAEKMAHLRSAIAGIEDQAGPRRRAALRLPVARALDGAL
                     GGGLAGDALHEVAPAGPGDGAAAMAFALCLAGRFCAASPRRGAALIAYEEFCVRETGA
                     LYGPGLAAFGLKRDQLLFVNAPDGRALLWTMEEALKSGALAVVVGELWSVVRWYGLAA
                     SRRLLLAARSGTTPALMVHGGLFGQAAALSTAAETRFEIAAATSRPLAPASGALALPG
                     APAFDVRLVKARLSPAAQGPPTRLFDETRVHRLEWSSVEKSFDDPTIPLPVASAPVDG
                     PRPRVAAA"
     gene            complement(2214..2822)
                     /locus_tag="VKU03_03585"
     CDS             complement(2214..2822)
                     /locus_tag="VKU03_03585"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HLJ70373.1"
                     /translation="MTSRLALILPFVAALGSAALLFPANAGAQQANHDQPAMDAAGPM
                     DDDGPMGMDHRMGMDRRPTPEDRQAFLDARIAAVHAGLQLTPEQEKLWPPVESAIRDS
                     ISQMREAWRKMKAGSGGMEKPDPIARMRRMAEMSTLVAQNLNKVADAAQPLYAALSDD
                     QKWRLHVLLRAMHMHGMHGMGMMRHRWGRGGGDMMRHGEDEE"
     gene            3061..3876
                     /gene="tam"
                     /locus_tag="VKU03_03590"
     CDS             3061..3876
                     /gene="tam"
                     /locus_tag="VKU03_03590"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HLJ70374.1"
                     /translation="METSETASPFVDANEWDVALYLKFEKERVRAATDLLARVPTQSP
                     RRIVDLGCGPGASTRLLATRFPAAEVIGLDSSDRMLASARARLPAIRFEKRDIGAWRP
                     PTPPDIIFANSSLQWLPDHHELAPRLYSLLAEGGSLAVQMPDNRHEPSHALMRMVAAD
                     GPWVDRLLPIAKTRAVIGAYSDYYRWLAPLGARVQIWQTTYVHALNGVGELVDWFRGS
                     GLRPFLNPLDECEREAFIARYMEELAAAYPAEPDGKVLFLYPRLFVVATKPGG"
     gene            3964..4545
                     /locus_tag="VKU03_03595"
     CDS             3964..4545
                     /locus_tag="VKU03_03595"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ70375.1"
                     /translation="MKARAEKAAATRERIVKSAVDLIVERTLDGLTLEAIAERAGVAA
                     RTILRIFGSKEQVFAAAINAEGRRGHGQLHPGDAEATIKVVFDDYEKIGDAVILRLAD
                     EGRIPSLDALLKSGRDNHRRWIEESFSPQLSKLDRNARAELLHALLVATDVYAWKLLR
                     RDFGLSRRAAEKVVGRIVRSLVEGGRQWRGFSG"
     gene            4524..5690
                     /locus_tag="VKU03_03600"
     CDS             4524..5690
                     /locus_tag="VKU03_03600"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ70376.1"
                     /translation="MARFLWLNWSGGGNLPPSLGVARALTERGHDVAFAGRPEMVPRV
                     DQAGFRAIELTRAYEQAELYPKDWPLWRMACYLSSPAVEEQVGAVIEAEAPDLLIVDQ
                     MLTAGLAQAAKSPLPTVMMCHTLFLRHIDQYRAFCARTNAARQAAGFAALPEADRLWF
                     ERDLIVTTSLASLDRRDPLPDGADKVRHVGPALAMERHAAPVDLPWSADDPTPLVLLS
                     FSTGPEQGNVDKMQRTLDALAGLNVHVVATGGRSIDIGKVAAPGNAVALDAADHDALM
                     ARSALVVTHGGHGTMMRALKYGLPMLVMPGMAADQAPNAAVVEEIGAGRSLSQDASVE
                     AIRAAAADVLETPAFGMKAREIARQMTGMDGALGAALEIEALLASPAAARRAVG"
     gene            5759..>6073
                     /locus_tag="VKU03_03605"
     CDS             5759..>6073
                     /locus_tag="VKU03_03605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003607161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLJ70377.1"
                     /translation="MPDKRKRADLLLVERGEFPSRARAQAAIEAGLVRVGGETLRKAS
                     AMIDADASIEAAAPHPWASRGGVKLAAALDAFTFDPAGLACLDVGASTGGFTDVLLAR
                     GAR"
BASE COUNT          997 a         2078 c         2055 g          943 t
ORIGIN      
        1 atcctccccc gcgcagcggg ggaggggaac cgcgcgcagc gcggtggagg gggcctgccg
       61 ccgcgccgga ctaggcgttc gcgcctcgcc caaagtcgcc ggaatcgcca tcacggcgaa
      121 ttcgggaatg tgcgtctcga cgcactcgat ccgcgtgacg cggcgcgcgc cgagccgggc
      181 gctcaagcgg tcgagaagat cggccaacgc gcggcgatcc tcctcatgcg gcggccgcgc
      241 gaaatcgccc tgcgccggcg cgagcggctc ggcgagaagg caggaaaggc gcagcacgtc
      301 gaagccgtag ccggagtcga attcgctctc ttcaggcgcc tcgaactttt cgcggaacag
      361 ccgcgcgatg gcgccggcct cgttgagcgg ccggctggcc ccgaccgcga tgcggcgcgt
      421 cgcgccgtcg acgcggaaca gagccagttc gagccggcgc gcgcctttgg cctggcgctg
      481 cagcaaggcg gccaggttct cggcgagctt gaagagcgcc gcctcgatcg ccgcgcgctc
      541 ttgaatcgcg ctggcgaagc gccgctcggc ggcaaaatcc ggcgcggcga agcgcggcga
      601 catcgcgccg cgctccaggc cgcagagcgc gtcgaaccgg gcgatcacgt cctcgccgaa
      661 gcgcgcggcg accggcgcgc gcgggcgcat ggcgatgtcg ccgatctggc gcaggcccgc
      721 gcgggccata tcggcggcga cgctctcgct taagcccagc gcggcgagcg gcaggccatg
      781 gtacaattgc gcgaaggatt ttccggcgac gccggccggc gcgaggcgct gccgggagaa
      841 gcgcgccagc gcccaggcgg cgcgcggaaa atcggcgatt cccgcccgcg cggcgaggcc
      901 ctggccggca aggcgcgtct cgatgtcccg cgccagggcc tcttcgccgc cgaagagatg
      961 ggcgacgccg gcaatgtcga gcatgatccc gtccgcgccg tcgagcgcga cgagcggcgt
     1021 gaagcgctgc agccaatcgg cgatgcgggc gagcagcttc ccctcttcga gaggattggc
     1081 ttcgacgacg acgagagcgg gtcggcgcgc tttggcgtcg gcgagcggca gcccgacggc
     1141 gaggccgagg ctcgccgcca gccgatcgac gcagacgagg cgctgcgcat tcttgatctt
     1201 ggcgtaggtc gcgagcggcg ccggcgaagg ggacgcgccg cgctcacgcc gcagcaacgc
     1261 gcgggcgcgg tccgtcgaca ggcgcggaag ccacagggag aggtatcgtc ggatcatcga
     1321 agcttttctc aacgctcgac cattcgagac gatgaacgcg cgtctcgtcg aaaagccgcg
     1381 tcggcgggcc ttgcgcggcg ggcgagagcc gcgccttgac cagccggacg tcgaaagccg
     1441 gcgcgcccgg caaggcgaga gcgccgctcg ccggggccag aggacggctc gtcgcggcgg
     1501 cgatttcgaa gcgcgtctcg gcggcggtgg aaagcgcggc ggcttggccg aagaggccgc
     1561 catggaccat cagcgccggc gtcgtcccgc tacgcgccgc cagcagcagc cggcgcgagg
     1621 cggcgaggcc gtaccatcgg acgacgctcc acaattcgcc gacgacgacg gcgagcgcgc
     1681 cgcttttcag cgcctcctcc atcgtccaga gcaaggcgcg cccgtccggc gcgttcacga
     1741 aaaggagctg atcgcgcttg aggccgaaag cggcgagacc cggcccataa agcgcgcccg
     1801 tctcgcggac gcagaattcc tcgtaagcga tcaaggccgc gccgcggcga ggcgaggcgg
     1861 cgcaaaaccg cccggcgagg caaagcgcga aggccatcgc cgccgcgccg tcgcccgggc
     1921 ccgccggcgc gacttcatgg agggcgtcgc cggcgaggcc cccgccgagc gcgccgtcca
     1981 aagcccgcgc gaccggcagg cgaagcgcgg cgcgacggcg cggcccggcc tggtcctcga
     2041 tcccggcaat ggcgctgcgc aaatgcgcca ttttttctgc gctgagaatt ggcggcatga
     2101 gctcgaacgc acaattactg ttccctgttt gttctataac aaggccaaac gccctgagag
     2161 tcaagcgttg attcgtcccg caagacgaat caacgctcta cccgcttaac gcctcactcc
     2221 tcatcctcgc cgtgacgcat catgtctcca ccgccccttc cccagcggtg acgcatcatg
     2281 cccattccat gcatgccgtg catgtgcatc gcgcgaagca gaacgtgcag acgccatttc
     2341 tggtcgtcgc tcaaagcggc gtagagcggc tgggcggcgt cggcgacctt gttcagattc
     2401 tgcgcaacca gcgtgctcat ttcggccatc cgccgcatcc gcgcgatcgg atcgggcttc
     2461 tccatcccgc cggacccggc cttcatcttc cgccacgcct cgcgcatttg gcttatcgaa
     2521 tcgcggatcg cgctttcaac cggcggccac aatttctcct gctccggcgt gagctggagc
     2581 ccggcatgca cggcggcgat gcgggcgtcg agaaaggcct gccgatcttc cggagtcggg
     2641 cggcgatcca tgcccatccg atgatccatg cccatcgggc cgtcatcgtc catcggaccg
     2701 gccgcgtcca ttgcgggctg atcatgattg gcttgctgcg cgccggcatt ggcggggaac
     2761 agaagagccg ccgatcccaa tgcggcgacg aaggggagaa taagggcgag tcgcgacgtc
     2821 atggctgtcg agctccttgc gacgatttcc gcttgatcgc gacaataaaa atctgccggc
     2881 agcccggcaa atcaagcttt agtcatcagg acgacaaagg cggcgcttag cgtacgtttc
     2941 cgcgctttcg ccggaggcgc gtcactttgg aaaggctcca aggggagcct gaaattggac
     3001 atattgcgct tccatctcgc gaagcatgat tgaagattgg gagggatgat ggcgcggccc
     3061 atggagacgt ccgaaaccgc ttcgccgttc gtcgacgcca acgaatggga cgttgcgctc
     3121 tacctgaaat tcgagaagga gcgcgtgcgc gcggcgacgg acctgctcgc ccgcgtgccg
     3181 acgcaatcgc ctcgacgcat cgtcgatctc ggctgcggac cgggcgccag cacgcgtctc
     3241 ctcgcgacgc gttttccggc ggccgaggtg atcgggctcg acagctccga ccggatgctg
     3301 gcgagcgcgc gggcgcgcct gccggcgatc cgtttcgaaa agcgcgacat cggcgcctgg
     3361 cggccgccga cgccgccgga catcatcttc gccaattcgt ccttgcaatg gctgcccgac
     3421 caccacgaat tggcgccgcg cctctattcg ctgctcgccg aaggcggctc gctcgccgtc
     3481 cagatgccgg acaatcggca tgagccgtcg catgcgctga tgcgcatggt cgccgccgac
     3541 gggccttggg tcgaccggct gttgccgatc gccaagaccc gcgcggtgat cggcgcctat
     3601 tcggactatt accgctggct ggcgccgctc ggcgcgagag tacaaatctg gcagacgact
     3661 tatgttcacg ccctgaacgg cgtcggcgag ctcgtcgact ggttccgcgg cagcggcctc
     3721 aggccgttcc tcaatccgct cgacgagtgc gagcgcgagg cgttcatcgc ccgctatatg
     3781 gaggagctcg ccgccgccta tccggccgag ccggacggga aggttctctt cctttatccg
     3841 cgcctgttcg tcgtcgcgac caagccgggc gggtgagtcc gctatttcgc gtcactcgta
     3901 ttgacgccaa atttatttca tgtcatattc agtgacatga atatagaaac gcgcgcttat
     3961 gtcatgaaag ctcgcgccga gaaggcggcg gcgacgaggg agcggatcgt caaaagcgcc
     4021 gtcgacctca tcgtcgagcg gacgcttgac gggctgaccc tggaggcgat cgccgagcgc
     4081 gcgggcgtcg ccgcgcgcac catcctgcgc attttcggca gcaaggagca ggtcttcgcc
     4141 gcagcgatca acgccgaagg ccgacgcggt cacggccaac ttcatccagg cgacgccgag
     4201 gcgacaatca aagtcgtctt cgacgattac gagaagatcg gcgacgccgt catcctgcgc
     4261 ctcgccgatg aaggccgcat tccctcgctc gacgctttgc tcaagtcggg gcgcgacaat
     4321 catcggcgct ggatcgagga aagcttctcg ccgcagcttt ccaagctcga cagaaatgcg
     4381 cgcgccgagc ttctccacgc gctgctcgtt gcgaccgacg tctatgcttg gaaattgctg
     4441 cgccgcgatt tcggcctcag ccgacgcgcc gccgaaaagg tcgttggtag aatagttcgt
     4501 tcgcttgtcg aaggagggag acaatggcgc ggtttctctg gttgaattgg tcgggcggcg
     4561 gcaatctgcc gccgagtctt ggcgtggcgc gcgcgctgac cgaacgcggg cacgacgtcg
     4621 ccttcgcggg acggccggaa atggtcccgc gcgtcgatca agccggcttc cgcgcgatcg
     4681 aattgacgcg cgcctacgaa caagccgagc tttatccgaa ggattggccg ctttggcgga
     4741 tggcctgcta tctctcctcg cccgccgtcg aggaacaagt cggcgccgtc attgaagcgg
     4801 aggcgcccga tctgctcatc gtcgaccaga tgctcaccgc tggactcgcg caagccgcga
     4861 agtcgccgct ccccacggtc atgatgtgcc acaccctgtt cctgcggcac atcgaccagt
     4921 accgcgcctt ttgcgcgcga acgaatgcgg cgcggcaggc ggcaggcttc gccgccctgc
     4981 ccgaggccga ccgtctgtgg ttcgagcgcg atctcatcgt gacgacctcg ctcgcatcgc
     5041 tcgaccgccg cgatcctttg ccggacggcg cggataaagt acgccacgtc gggccggccc
     5101 tcgcaatgga gcgccatgcg gcgcccgtcg atcttccctg gagcgcggac gatccgacgc
     5161 cgctcgtcct gctcagcttc tcgaccggtc cggaacaggg caatgtcgac aagatgcagc
     5221 ggacgctcga cgcgcttgcc ggcctcaacg tccatgtcgt cgccaccggc gggcgcagca
     5281 tcgacatcgg caaagtcgca gcgcccggca acgccgtcgc gctcgacgcc gccgatcacg
     5341 acgccttgat ggcgcggtcc gcgctggtcg tcacgcatgg cggccatggt acgatgatgc
     5401 gcgctctgaa atatggcctg ccgatgctgg tcatgcccgg catggccgcc gaccaagcgc
     5461 cgaatgcggc cgtggtggag gagatcggcg cgggacgctc gctctcgcag gacgcgagcg
     5521 tcgaagcgat ccgcgccgct gcggccgacg tgctggagac ccccgctttc ggcatgaagg
     5581 cgcgcgaaat cgcgaggcaa atgacgggca tggacggcgc tctcggcgca gcgctggaaa
     5641 tcgaggcgct gctcgcttca cccgccgccg cgcgccgcgc cgtgggttga tcggagcgcg
     5701 aagacgcgtc aataaggcgg ctgcccgtgt tggaaatgac ggaacgggca gattcgccat
     5761 gccggacaaa cgcaagcgcg ccgatcttct cctcgtcgaa cgcggcgagt ttccgagccg
     5821 cgcccgcgcc caggcggcga tcgaagccgg gctggtgcgg gtcggcggcg agaccttgcg
     5881 caaggcctcg gcgatgatcg acgccgacgc ttcgatcgag gccgcggcgc cgcatccatg
     5941 ggcgtcgcgc ggcggcgtca agctggcggc ggcgctcgac gcgttcactt tcgatcccgc
     6001 cgggctcgcc tgtctcgatg tcggcgcctc gaccggcggc ttcaccgatg tcctgctcgc
     6061 ccgcggcgcg cgc
//
LOCUS       DATGJW010000123         3159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1370392, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000123 DATGJW010000000
VERSION     DATGJW010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3159
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1370392"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1196)
                     /locus_tag="VKU03_03610"
     CDS             complement(<1..1196)
                     /locus_tag="VKU03_03610"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HLJ70378.1"
                     /translation="MKKVFLTSTCAAAISVFAAWTFSGASYAQQSQNTGASQQETQHK
                     TGAESKEMKPGATKAGGAMNGAANENKEGTKNMNAAEERQNGESNKAGAMKRHESTTA
                     EQKGQTENGKMQSGEANQQEKMKSGQANKQEEKTQTGQAKQEEKTHTGAAGQKEPMRS
                     GEASQKEHMKSGAAEEKGKAQTGAAEEKGKTQNGAAAQSETSKSRMQTGETSQKRATE
                     GRAAAEPGNAGATGHEAMAHGKMKMNPQQASDFRSRLEKHGEVTRSSVNFNVNVGVAV
                     PESVRLEPIPTDIIVEYPEFRGYDVVMVQDEIVIVDPHTRQVVEVVGGSTNSASVGRR
                     ERLRLTREQDQVIRRHVHGRPEKTVEFDERIDARVPDSVMLEPLPGPVVAEIPEVRSY
                     DYFVDR"
     gene            1428..1652
                     /locus_tag="VKU03_03615"
     CDS             1428..1652
                     /locus_tag="VKU03_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70379.1"
                     /translation="MNKEKQAGEAPPPETGREIWTCPFCQQTVRVGAYGCCAPPDFVK
                     NCLLREHMVGDECIAYLNPRKARELMMERD"
     gene            1832..2881
                     /locus_tag="VKU03_03620"
     CDS             1832..2881
                     /locus_tag="VKU03_03620"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLJ70380.1"
                     /translation="MRRGVGPWEDIRNRLLRRGIQAVCGAPDFVSTSAAADISLLSGG
                     LRFAFVRALGALGVESFVARSGLGYDFLCHTGDLSEYPFYHRRAFEKELAICVGWLDQ
                     EDAPIVYDLGANVGFVSTHLAQMLADRSPTIYAFEPAPETYRRLVASVERLGLTGRVQ
                     PFLAAATDRRGKVRIAYEKGNSLHTRVLSDGADDASAGSAIWAAGVTLDEFSASRGAP
                     PALIKMDIEGSEVAALRGAAALLSQRDPPAILFEHNPVSLAQCGVGIAALYQLLADYT
                     FYYIDDLRGQIAPMGSPVVSFGRIDWICNIFAAPRGAASAARWAAALRHAQQRLAARE
                     RRRTHRMEWRNAAAE"
     gene            2957..>3159
                     /locus_tag="VKU03_03625"
     CDS             2957..>3159
                     /locus_tag="VKU03_03625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70381.1"
                     /translation="MTNRQGIAPRPAWLSRRAILRAATATVLSARLTGANAAMTTPFR
                     PGLAPPMTGREDFVRWMAANRGE"
BASE COUNT          438 a         1056 c          995 g          670 t
ORIGIN      
        1 ctacggtcga cgaaataatc gtagctccgc acttccggaa tctccgctac gaccgggccg
       61 ggcaatggct caagcatgac cgagtcggga acgcgcgcat cgatccgttc gtcgaattcg
      121 accgtcttct ccgggcggcc atggacgtgc cggcggatga cttgatcctg ctcgcgcgtg
      181 aggcgcagcc tttcgcgacg gccgaccgag gccgagttgg tcgacccgcc gacaacttcc
      241 acgacctggc gggtatgcgg atcgacgatc acgatctcgt cctgcaccat caccacgtca
      301 tagccgcgga actccggata ttcgacgata atatcagtcg ggatcggctc gagccggacg
      361 gactccggca ccgccacgcc aacattcacg ttgaagttga cagagctcct cgtcacctcc
      421 ccgtgcttct cgagacggct gcggaagtcc gaggcctgct gcgggttcat cttcatcttt
      481 ccgtgcgcca tcgcctcgtg acccgtcgcg ccggcgtttc ccggctccgc cgcggcgcgc
      541 ccttcggtcg cgcgcttttg cgacgtttcg ccggtctgca tgcgggattt cgaggtttcg
      601 gactgagccg cggcgccgtt ctgagtcttg cccttctctt ccgcggcgcc ggtctgcgct
      661 ttgcccttct cctccgcggc tccgctcttc atatgctctt tctggctggc ttcgccgctc
      721 cgcatcggct ctttctggcc ggccgctccg gtatgggtct tctcttcctg cttggcctgg
      781 ccggtctggg tcttctcttc ctgtttattg gcttggccgc tcttcatctt ctcttgttga
      841 ttggcctcgc cgctctgcat cttgccgttc tcggtctgac ccttctgctc ggcggtggtc
      901 gattcgtggc gcttcatcgc gccggctttg ttcgactcgc cgttttgccg ctcctcggcg
      961 gcgttcatat tcttggtccc ctctttgttt tcgttcgccg cgccgttcat cgcgccgccg
     1021 gccttggtcg cgccgggctt catctccttc gattcggcgc cggttttgtg ctgagtctct
     1081 tgctggctcg cgccggtatt ctggctttgc tgggcgtagc tcgcgccgct gaacgtccag
     1141 gcggcgaaaa cggaaatcgc tgccgcgcaa gtggacgtca aaaatacctt cttcatggat
     1201 cgcctccgca tatcggaaat tggatcaggc aacgggcaag ccccaccgcc ggcgccgcgc
     1261 acgacgcgcg ccggacgctc ggacgagccc gttttcctcc tcgcaaaacc gtccgccgat
     1321 gcgggagttc cggcttcgcg ttaatttgcc gggccagaaa gttgggtctc ctgcgcaaca
     1381 gaggcggccg ccagacgtta tttccgggaa gacgagacgc gtcggccatg aacaaggaaa
     1441 aacaagccgg ggaggccccg ccgcccgaaa ccggccgcga aatttggacc tgtcccttct
     1501 gtcagcagac ggttcgcgtc ggcgcctatg gctgttgcgc gccgccggat ttcgtcaaga
     1561 actgcctgct gcgcgagcac atggtgggcg acgagtgcat cgcctatctc aatccgcgca
     1621 aggcgcgcga gttgatgatg gagcgagact agacgtccga cgccattgtg cggcgcgcca
     1681 taccgacttt ggaacaaagg gcgtaggccg ggcgattgtg ttgcaatttt cctccggctt
     1741 cacggacctc catggcgtcc tcgctctttt gcggcgtccg ccggcttgcg gcttggcggg
     1801 ctgtacttcg tcaattcgcc cggcggctct aatgcggcgc ggcgtcggcc cgtgggaaga
     1861 catccgcaat cgcctgctgc ggcgcggcat tcaggccgta tgcggcgcgc ctgatttcgt
     1921 ctcgacgagc gccgcggcgg acatttcttt gctgagcggc ggcttgcgtt ttgctttcgt
     1981 gcgggcgctc ggcgcgctcg gcgtggaaag cttcgtcgcc aggagcgggc tgggctatga
     2041 cttcctgtgc catacgggcg acctctctga atatccattc tatcatcggc gcgcgttcga
     2101 gaaggagctg gcgatctgcg tcggctggct cgaccaggag gacgcgccga tcgtttacga
     2161 cctcggggcc aatgtcggct tcgtttcgac gcacttggcg cagatgctgg cggaccggtc
     2221 gccgaccatc tacgccttcg agcccgcgcc ggagacatat cgacggctcg tcgcctcggt
     2281 cgaacgcctc ggtttgacgg gtcgcgttca gccgtttctc gccgcagcga cggacaggcg
     2341 agggaaagtg cgcatcgctt atgagaaggg aaactccttg catacgcggg ttttgagcga
     2401 cggcgccgac gacgcctcgg caggatcagc gatttgggcc gccggcgtga cgcttgacga
     2461 attcagcgct tcgcgcggcg cgcctccggc cttgatcaaa atggacatcg agggcagcga
     2521 ggtcgccgcg ctccggggcg cggccgcgct gctttctcag cgcgatccgc cggcgatcct
     2581 tttcgagcac aatccggtct ctctcgccca atgcggcgtc ggaatcgcgg cgctttacca
     2641 gcttttggcg gactacacgt tctactacat cgacgatttg cgcgggcaaa tcgcgccgat
     2701 gggcagcccg gtcgtctcgt tcgggcgcat cgactggatc tgcaacatct ttgccgcgcc
     2761 gcgcggggcg gcttccgcag cgcgctgggc cgcggcgctc cgtcatgcgc aacagcgcct
     2821 tgccgctcgc gagcggcggc ggacgcatcg gatggaatgg cggaacgcag ccgccgaatg
     2881 acacgccgct tccgcggagc gatttgacgg cgccaactcg gcgcccgatg cttcgccgcg
     2941 tttttagggc tcgtccgtga ccaaccggca aggcatcgct ccccgtcccg cctggctcag
     3001 ccgccgcgcc atcctccggg ctgcgacggc gacggtttta tcggcgcgcc tgaccggcgc
     3061 gaacgccgcg atgacgacgc cttttcggcc gggcttggcg ccgcccatga ccggccggga
     3121 ggatttcgtg cgctggatgg ccgccaatcg cggcgaaga
//
LOCUS       DATGJW010000124         6463 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_748145, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000124 DATGJW010000000
VERSION     DATGJW010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6463)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6463)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6463
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748145"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1130)
                     /locus_tag="VKU03_03630"
     CDS             complement(<1..1130)
                     /locus_tag="VKU03_03630"
                     /inference="COORDINATES: protein motif:HMM:NF014066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TraB/GumN family protein"
                     /protein_id="HLJ70382.1"
                     /translation="MSGRASSESGADGAAGVCPAGRRASLSPWSEAERGEVRRGAGRS
                     AATSEAYSPGPSPYLSPLRGERGVDASLHPQPLSAGATRRASRFWLHAFAAVLLLWPA
                     ISFADAPPACHGRDLSSLAASRPDALKSAEQARADWLVNAQGLLWRIDKPGVASSYLF
                     GTIHSSDDRAIALAHKAAAEIKGAKVVATELGGPIDKAEMAEYGAKLLVKALAQDIDT
                     FEPIAAGDRAAVEHYLAARGVNTELAHHIQLWFLTATTAAPVCEARRQAVGLPIVDNV
                     IAQAAKDSGVKVEALETFDEQGSTLAALKPADAATLLVGGAKKPGLDEDAYATLLDLY
                     AAGRPVEAMPIIDASGLLSKEEIAAEDTFSLLLLGERNRIMA"
     gene            complement(1127..2095)
                     /gene="coaA"
                     /locus_tag="VKU03_03635"
     CDS             complement(1127..2095)
                     /gene="coaA"
                     /locus_tag="VKU03_03635"
                     /EC_number="2.7.1.33"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004594 - pantothenate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I pantothenate kinase"
                     /protein_id="HLJ70383.1"
                     /translation="MSEPAFAAPPEKVASPYHRFTRDEWAKLRADTPLTLTIEDLRRL
                     QSLHDPISLEEVIAIYLPLSRLLALYVAATQGLFKATQRFLGAADGKVPYIIGIAGSV
                     AVGKSTMARVLKALLSRWPNTPKVELVTTDGFLKPNAALVRENLMERKGFPESYDGSA
                     LIRFLSDVKAGKRNVMAPVYSHLVYDVVPGEHIFVDRPDILIVEGLNVLLPNRLSRGD
                     KEIPFVSDFFDFSIFLDADDALLEKWYIARFMTLRETAFRDERSYFHKFADLGDEEAE
                     ATARSIWRRINLVNLHENILPTRPRASLLLTKGPSHRIEEVALRKL"
     gene            complement(2218..2535)
                     /locus_tag="VKU03_03640"
     CDS             complement(2218..2535)
                     /locus_tag="VKU03_03640"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HLJ70384.1"
                     /translation="MTFSLDDLARIIAERASATAEKSYTRSLLDNGPKGAAKKFGEEA
                     AEFVIAAVSEDETALRAEAADVLYHLLVVLEARGAKLADVLAELQRRTQRSGHDEKAA
                     RAK"
     gene            2675..2968
                     /locus_tag="VKU03_03645"
     CDS             2675..2968
                     /locus_tag="VKU03_03645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010685564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLJ70385.1"
                     /translation="MLGLEVRQLAVFVSWKERLRDRAAVARINVRISRLALGNFGDVR
                     PIGEGVSELRINFGPGYRVYCTQRGDAVTLLCGGDKSSQESDIRRAKKLAKEI"
     gene            2970..3260
                     /locus_tag="VKU03_03650"
     CDS             2970..3260
                     /locus_tag="VKU03_03650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="addiction module antidote protein"
                     /protein_id="HLJ70386.1"
                     /translation="MPLETTRWDPVDDLKSPEDERLYLEAAFEDGDPAIIAAALGDVA
                     RARGMAKIAEEAGLTRDALYKSLKSGGNPTLATLIRVADALGYRLAIQAKAA"
     gene            complement(3304..4398)
                     /locus_tag="VKU03_03655"
     CDS             complement(3304..4398)
                     /locus_tag="VKU03_03655"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ70387.1"
                     /translation="MSTNVLAASSEKDGAAAKSDPAHEAKPWSDPQGLAQSLRAAAET
                     MRNALSGAEPARRAAPRVAVILPCYNEAPAIGGVVGEFKTALPGARIVVFDNGSRDET
                     AAIARAAGAEVYFEPRPGKGNVVRRMFADIDADIYVMADGDGTYDAAAAPRLVAAIAE
                     DNVDMAVGARAEIYDEAHRRGHAIGNFVFNRLYGTLFGAGFADIFSGYRAFSRRFVKS
                     FPALSSGFEIETELSVHASQLRLPTAEIVLPYRQREEGSASKLRTIRDGARILRTFAL
                     LLKETRPALFFGSLAAATMLAAIVLAAPVFATYAETGLVPRLPTVVLCTGLALCAALM
                     GVCGLILDSLARARVEQKRILYLAMAAPRG"
     gene            4570..6387
                     /locus_tag="VKU03_03660"
     CDS             4570..6387
                     /locus_tag="VKU03_03660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70388.1"
                     /translation="MSLAAARPSALSARGEAGFSLLRSPAGLAVLALILSIVKFWPAI
                     VSVWTTGAFANPDDAMRLVEVRDLIAGQNWFDLHQYRLDPPAGVDMHWTRVLDLPLAL
                     LIKSFSLALPVEAAERLTRIVFPLALLFALYGAIFSLARRLAGPAALLPAMIIAILAG
                     AMLGQFEPGRIHHHIAQILLAVLILRATIEAVWAKSVGRAAFAAALAALSLSINIENI
                     TYILVEIAVFALVFVAEGEAFAAALAGFAVSLAVSSLLLFAATVGPSHYFLGACDAFS
                     TPHLLAILIGAAALLAMAAGAGRLAGWPLRLAACALGGAAVVAALASAYPACLADPQA
                     GVDPLLRLYWLSHVKEARPLLAMIRATPSDFFLFALAPILGLLAALAAAWRSGGASRA
                     AWLIVAAFAAIGLATSSWQVRAISSTAALALFGGVWASAQVLEWAERKKNLLAKAAPI
                     AAILPFCSIFWALLVAVFAPEPAYANAAAAACRSPATIHALDALPKSLIMAAIDMGSD
                     ILAETDHSVLAAPYHRNNRGNGEFVRAMIATPDAARKIAEDSGAAYLVFCPARAEFDD
                     YVEGSPDGLAAVLRKGDAPDWLSPVAVPPGSPLQIYRIR"
BASE COUNT         1003 a         2150 c         2161 g         1149 t
ORIGIN      
        1 tcggccatga tccgattgcg ctcgccgagc agaagcaggc tgaacgtatc ttccgccgcg
       61 atctcctctt tcgagaggag cccgctcgcg tcgatgatcg gcatcgcttc gaccggccgt
      121 ccggcggcgt agagatcgag cagagtggcg taggcgtctt cgtcgaggcc gggctttttc
      181 gcgccgccga cgagaagcgt cgccgcgtcg gcgggcttga gcgccgcgag ggtcgagcct
      241 tgctcgtcga acgtttcgag cgcctcgacc ttgacgcctg aatccttcgc cgcttgcgcg
      301 atgacgttgt cgacgatcgg caagccgacc gcctgccgcc gcgcctcgca gaccggcgcg
      361 gccgtcgtcg cggtcaggaa ccaaagctgg atatgatggg cgagctcggt attgacgccg
      421 cgcgcggcga gataatgttc gaccgcggcg cggtcgccgg cggcgatcgg ctcgaacgtg
      481 tcgatatcct gcgccagggc tttgaccagc agcttggcgc cgtattcggc catctcggcc
      541 ttgtcgatcg gcccgcccag ctcggtggcg acgactttcg cgcccttgat ctcggccgcc
      601 gccttatggg ccagcgcaat ggcgcggtcg tcgctcgaat ggatcgtgcc gaaaaggtac
      661 gacgacgcga cgccaggctt gtcgatccgc cacagcaggc cctgggcgtt gaccagccaa
      721 tcggctcgcg cctgttcggc gcttttcagc gcgtcaggcc gtgaagcggc gagggaggaa
      781 aggtcgcggc cgtgacaggc cggcggggcg tcggcgaagc ttatcgcggg ccagaggaga
      841 agaacggcgg cgaacgcgtg tagccaaaag cgcgaggcgc gccgcgttgc gccggccgag
      901 agcggttgag gatgaagcga ggcgtcgacg cccctctccc cgcgcagcgg ggagaggtac
      961 ggcgaggggc cgggggagta agcctctgac gtcgcggctg agcgacccgc ccctctccgt
     1021 acctctcccc gttccgcttc gctccacggg gagagggagg cccggcgccc agccgggcag
     1081 acgcctgccg cgccgtcggc gccgctttcg cttgaagcgc gtccgctcat agcttgcgca
     1141 gcgccacttc ttcgatccga tggctcggcc ccttggtcag gagcaagctg gcgcgcggcc
     1201 gcgtcggcag gatgttctcg tgcaggttga cgaggttgat ccgccgccag atcgagcggg
     1261 cggtcgcttc cgcctcctcg tcgccgagat cggcgaattt gtggaagtag gaccgctcgt
     1321 cgcggaacgc cgtctcgcgc aaggtcatga agcgggcgat gtaccatttt tcgagcagcg
     1381 cgtcgtcggc gtcgaggaag atcgagaaat caaagaaatc cgagacgaag ggaatctcct
     1441 tgtcgccccg gctgaggcga ttcggcaaga gcacgttcag gccctcgacg atgagaatgt
     1501 ccggccggtc gacgaaaata tgctcgccgg gcacgacgtc gtagacgaga tgcgaataga
     1561 ccggcgccat gacgttgcgc ttgcccgcct tcacgtccga gaggaagcgg attaaggccg
     1621 agccgtcata gctttccggg aagcccttgc gctccatcag gttttcccgg acgagcgcgg
     1681 cgttcggctt taaaaagccg tcggtcgtca cgagttcgac tttcggcgtg ttgggccagc
     1741 gcgacagcag cgccttgagc acgcgcgcca tggtcgattt gccgaccgcc accgagccgg
     1801 ctatgccgat aatgtacgga accttgccgt ccgcggcgcc gagaaatcgc tgcgtcgcct
     1861 tgaacaggcc ttgcgtcgcc gcgacataga gggcgagcag gcgcgatagg ggtagataga
     1921 tcgcgatcac ctcttcgagc gagatcggat cgtgcagcga ttgcagccgg cgcagatcct
     1981 cgatggtcaa ggtcagcggc gtatcggcgc gcagcttcgc ccattcgtcg cgggtgaagc
     2041 ggtgataggg cgaggcgacc ttttccggcg gcgcggcgaa agcgggttcg ctcatcgggt
     2101 cggaacgtct tcggtcccgc aagcgggttg tggccgcggt cggcgaccgc ggctacaggg
     2161 agagggctgg ggcaagggca agccgtcgga atgggtcact tgaaaccgcc tcaagcgcta
     2221 ttttgcgcgc gccgcctttt cgtcgtgtcc tgagcgctgg gtgcgccgct gcaattcggc
     2281 gagcacgtcg gcgagcttgg ccccccgcgc ctccagcaca accagcaaat gatatagcac
     2341 atcggcagcc tcggcgcgca gggctgtctc gtcttccgaa accgccgcga taacgaattc
     2401 cgccgcctcc tcgccgaatt tcttggccgc gcccttcggc ccgttgtcga gtagcgatct
     2461 cgtataggat ttctcagccg tcgccgaggc gcgctcggcg atgatgcggg cgagatcgtc
     2521 gagagaaaac gtcaagtgaa actccgcggc tccacatttc gcccgccggc cgaaaatcgt
     2581 gagcttctta gagtaatgcg ccgcctggcg acacttattc aagacaaatc cgcggaagaa
     2641 tgtcgttgac ggggtctgtg atgtatatta cattatacta gggctcgaag tccgacaact
     2701 cgctgtgttt gtctcctgga aggagcggct gcgcgatcga gcggcggtgg ctcggatcaa
     2761 cgtccgaata tcgcgcctcg ctcttggcaa ttttggcgac gtacgaccga tcggcgaggg
     2821 agtcagcgag ctgcggataa atttcggacc gggctatagg gtctattgta cgcagcgagg
     2881 cgacgccgtc accctgcttt gcggcggcga taaaagctcg caggaatccg atatacgtcg
     2941 agcgaagaag ttggcgaagg agatttgata tgcctctcga gacgacgcga tgggatcctg
     3001 tggatgatct caagtctcct gaagacgagc ggctctatct cgaggccgcc ttcgaggatg
     3061 gcgatcctgc aatcatcgcg gcggcgctcg gcgacgtggc tcgcgctcgc ggcatggcga
     3121 aaatcgccga ggaggcgggc ctcacccgag acgcgctcta caagtcgctg aagagcggcg
     3181 gcaatccgac tctcgccacg ctgatcagag tggcggacgc gctcggctac aggctcgcga
     3241 tccaggccaa ggcggcctaa tcgtccgccg cccgtgcgca attcgaccgc tgtccacccg
     3301 ccctcacccg cgcggcgccg ccatcgccag gtacaaaatc cgcttttgct ccacccgcgc
     3361 ccgcgccagc gaatcgagga tcaggccgca aacgcccatc aaagccgcgc agagggcgag
     3421 gccggtgcag aggacgacgg tcggcaggcg cggcaccagg ccggtctcgg cgtaggtggc
     3481 gaagaccggc gcggcgagga cgatcgccgc cagcatggtc gcggcggcga gcgagccgaa
     3541 gaacaaggcc ggccgcgtct ccttgagcag cagcgcgaaa gtgcgcagga tgcgcgcgcc
     3601 gtcgcggatc gtgcgcagct tggaggcgga gccttcctcg cgctggcggt agggcaggac
     3661 gatctccgcg gtcggcaggc ggagctggct ggcatggacc gagagctcgg tttcgatctc
     3721 gaagcccgag gacaaagccg ggaagctttt gacgaagcgg cgcgagaagg cgcgatagcc
     3781 ggagaaaata tcggcgaagc cggcgccgaa cagcgtcccg tagaggcggt tgaacacgaa
     3841 attgccgatc gcatggccgc gccgatgcgc ctcgtcgtaa atctcggcgc gggccccgac
     3901 cgccatatcg acattgtcct cggcgatggc ggcgacgagg cgcggcgccg cggcggcgtc
     3961 gtaggtgccg tcgccgtcgg ccatcacata aatgtcggcg tcgatgtcgg cgaacatccg
     4021 tcgcaccaca ttgcccttgc ccggccgcgg ctcgaagtag acctcggcgc cggcggcgcg
     4081 cgcgatcgcg gcggtctcgt cgcgggagcc gttgtcgaag acgacgattc gggcgccggg
     4141 cagcgcggtc ttgaattcgc ccacgacgcc gccaatggcg ggggcttcgt tgtagcacgg
     4201 caagatcacc gcgacgcgcg gcgcggcgcg tcgcgccggc tcagccccgc tcagcgcgtt
     4261 gcgcatcgtc tcggcggcgg cccgtagcga ttgcgccaag ccttgcgggt cggaccaggg
     4321 cttggcctcg tgcgccggat cggatttcgc agccgcgccg tccttctcag aactcgcggc
     4381 caggacattc gtcgacattg ttttcagcgc cggggtgagg ttgatcggcc tggctaacgc
     4441 tctttcaatc attggctggg aaaagttgac ccttggtgaa aggcggctca gccgcggcgc
     4501 gcggctctcc tggacggcct tgcccgggga tcgccgcgcg cccgacgccg cgacccaagg
     4561 gaccccttca tgtcgctcgc cgccgcccgt ccctccgccc tgtccgcgcg cggcgaggcc
     4621 gggttttcgc tgctgcgctc gccggccggg ctcgccgtcc tggcgctgat cctctcgatc
     4681 gtgaaattct ggccggccat cgtctcggtc tggaccaccg gcgctttcgc caatcccgac
     4741 gacgccatgc ggctcgtcga agtgcgcgac ctcatcgccg gccagaactg gttcgacctt
     4801 catcaatacc ggctcgaccc gccggccggc gtcgacatgc attggacgcg cgtgctcgat
     4861 ctgccgctgg cgctgctgat caagagcttc tcgctcgccc tgccggtcga agcggccgag
     4921 cggctgaccc gcatcgtctt tccgctggcg ttgcttttcg cgctttacgg cgcgattttc
     4981 agcttggcgc gccggctggc tggccccgcc gccctgctgc cggcaatgat cattgcgatt
     5041 ctggcgggcg ccatgctcgg gcaattcgag cccggccgca tccatcatca catcgcgcag
     5101 atcctgctcg ccgtcctcat ccttcgcgcg acgatcgagg cggtctgggc caagagcgtc
     5161 ggccgcgccg cgttcgccgc cgcgctcgcg gcgttgtcgc tgtcgatcaa catcgagaac
     5221 atcacctata ttctcgtcga gatcgccgtc ttcgccctgg tcttcgtcgc cgagggcgaa
     5281 gcgttcgccg cggcgcttgc cggtttcgcg gtctcgctcg ccgtctcctc gctgctgctt
     5341 ttcgccgcga cggtcgggcc gtcgcattat tttctcgggg cctgcgacgc cttctcgacg
     5401 ccgcatcttc tggcgatttt gatcggcgcc gccgcgctcc tggcgatggc ggccggcgcc
     5461 ggacggctcg ccggctggcc gctccggctc gccgcctgcg cgcttggcgg ggcggccgtg
     5521 gtcgccgccc tcgcttccgc ctatccggcc tgcctcgccg acccgcaagc cggcgtcgat
     5581 ccgctgctgc ggctttattg gctcagccat gtcaaagagg cgcgcccgct ccttgcgatg
     5641 atccgggcga cgccgagcga tttcttcctc ttcgccttgg ccccgattct ggggctgctc
     5701 gcggccctcg ccgccgcgtg gcgaagcggg ggcgcttccc gcgcggcctg gctgatcgtc
     5761 gccgccttcg ccgcgatcgg ccttgcgacc tcgtcctggc aggtgcgggc gatttcctcg
     5821 accgccgcct tggcgctgtt cggcggcgtc tgggcctcgg cccaggttct ggaatgggcg
     5881 gagcggaaaa aaaatcttct cgccaaggcg gcgccgatcg ccgcgattct gcccttctgc
     5941 tcgatctttt gggcgctgct tgtcgccgtc ttcgcccccg agccggcgta cgccaacgcg
     6001 gccgccgcag cctgccgcag cccggcgacg atccacgcgc tcgacgccct tcccaaatcc
     6061 ctcatcatgg cggcgatcga tatgggctcg gacattctcg ccgagaccga ccattcggtc
     6121 ctcgccgcgc cgtatcatcg caacaatcgc ggcaacggcg aattcgtccg cgccatgatc
     6181 gccacgcccg acgcggcgcg aaagatcgcc gaagacagcg gcgccgccta tctggtcttc
     6241 tgtccggccc gcgccgaatt cgacgattat gtcgagggca gtccggacgg tctggcggct
     6301 gtgctgcgta agggcgacgc gccggattgg ctgtcgccgg tcgccgttcc gcccggctcg
     6361 cctttgcaaa tctaccgcat ccgctgaacg cggacgcaga tcgcgaaaac gtgatctgct
     6421 cagtttagaa taaggaaaat ctggatttcc gaaacggctc acg
//
LOCUS       DATGJW010000125        11675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_22612, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000125 DATGJW010000000
VERSION     DATGJW010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11675
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22612"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            149..466
                     /locus_tag="VKU03_03665"
     CDS             149..466
                     /locus_tag="VKU03_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70389.1"
                     /translation="MRQRAEHWVTIRIAAEEAGRSEERRLQQEYSLHLESEGRPDGAV
                     IYWRGEEDGGRVYYFSPQAAVIAGAALERFSPRPISPDNMQALRARGMSVANWRHSYM
                     HPR"
     gene            complement(485..901)
                     /locus_tag="VKU03_03670"
     CDS             complement(485..901)
                     /locus_tag="VKU03_03670"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLJ70390.1"
                     /translation="MPASFTRKLTVEWGHCDPAGIVFNPRFFEYFDWSTSLLVREALG
                     VEKAAMRTIYGVAGIPLVDTRATFHRPARYGDEIEIVTAVAAVGRSSFEVEHKLFNKG
                     ELAVEARETRVWVVEEADGRLKSRPMPEDVARRLRT"
     gene            complement(1085..2335)
                     /locus_tag="VKU03_03675"
     CDS             complement(1085..2335)
                     /locus_tag="VKU03_03675"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HLJ70391.1"
                     /translation="MAETHPGVFSTWDDVYLAGGARTPFADLNGPLAPVSPIDLGIKA
                     GRAALEKSGVSGEDIGTIIAGSMAQASFDAYMTPRHIGLYCGAPEHVPAHLVQRICGT
                     GVEAVSQACDAIAHKGVEAALCVGTESMSRNPVAAYTHRGGFRMGQVEFKDFLWEALL
                     DTSCDVTMGGTAENLARRYQISRTEVDAYAERSFERALSARADGFFADEIVPVSSEIF
                     RCDGRKDRGVALDRKIERVAEDTHPRPTTRETLAKIRPAFGGVQTGGNSSGVVDGAAG
                     AVVASGAYLKAKGKRPLARLIGAVAVGCPPEIMGIGPAPAIRALLERVGMRLDRIDRI
                     EINEAFGAQVLACIRELGLDEDKLNVNGGAIAIGHPLGATGVRLTLTLARELQRRRLR
                     YGIASACIGGGQGIALLIENVAAA"
     gene            complement(2339..4231)
                     /locus_tag="VKU03_03680"
     CDS             complement(2339..4231)
                     /locus_tag="VKU03_03680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="feruloyl-CoA synthase"
                     /protein_id="HLJ70392.1"
                     /translation="MAGAASAIGPKLDPRTAPLRPFSIGSNAARFETRGVGAILVTPV
                     EKLGPHPEKLTERLAHFASVAPDRLWMAERAGADEWRKVSYGEAYRAARTIGAELLRR
                     GLSAERPVMILSGNDIEHALLALACLHVGVPYAPISPAYSTIAGDFSKLAHIAGKLTP
                     GLVFACDGALYGCALAAVAPEDAEIVVTRNPPDGRRATAFADLLAAAPDDAAVARAHA
                     AIRGADVAKILFTSGSTALPKGVINSQLMLTANQEMIANYFRFLKEQPPVLVDWLPWN
                     HTFGGNHNFGLVLYNGGSLYIDEGRPTPKGIEATIRNLREIATTVYFNVPKGFEELAP
                     RLRADRALRETFFSQLNMNFFAGAGLAQHVWDEIDAAAVETLGQRVPMMTGLGATETG
                     PFALACSPEHSKSGHLGLPVPGVELKLTPVDGKWEARVRGPAVTRGYWRDPEATAKAF
                     DEEGWYRMGDALKFIDERDRAKGFLFDGRIAEDFKLATGTWVSVGPLRARTIAHFAPL
                     FRDVAVAGLNRDDIAILAFPDIDGCRSFCGEPGAAEIGAILSHPALVAEIQRRLNAFA
                     AAATGSSNRVARLLLLEEPPSLDRGEITDKGSINQRAVLSCRAALVDELYARPFSSRV
                     ICASEA"
     gene            complement(4337..5140)
                     /locus_tag="VKU03_03685"
     CDS             complement(4337..5140)
                     /locus_tag="VKU03_03685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665197.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="crotonase/enoyl-CoA hydratase family protein"
                     /protein_id="HLJ70393.1"
                     /translation="MIVSSAVQAPELVSTRIEGEIAILRLERPQKRNALNDQAILALE
                     AAFRNLPPGVRAAVLHGAGEHFSAGLDLSELSEKDLNQAIEHSRLWHRAFEAIEFGRV
                     PVVSMLHGAVVGGGLELAAATHVRVAERSAFYALPEGQRGIFVGGGGSVRLPRLIGAS
                     RMMEMMLTGRVYRAEEGYPFGFSHYVVADGQGLAKAVELARAIAGNAGRTNFALIQAL
                     PRIADGDRAAGYLIEALMSAIAQDDAEAKARMRAFLDKRAGRVAPKEPN"
     gene            complement(5240..6190)
                     /locus_tag="VKU03_03690"
     CDS             complement(5240..6190)
                     /locus_tag="VKU03_03690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70394.1"
                     /translation="MNAALTRLLAKPWLWSFLGALVVFLASIAFTGGYGASGMVNAAF
                     SLAVFTVIVGVGQMFVITLGPGNVDLSLPANIGLASAVAMKVMNGDDSLVALGILAAL
                     GCGLGIGVANYLLIWALRIPPIIATLSASFVIQSIDISYGRGLQIKPPPGFANFANAQ
                     VFGAPILAILVVLFTIAAAIVLQRTIYGRSVLAIGQNIRAARLAGVKVGRTRFLTYAL
                     CGVLGGLDGALLAGYFRGANVDIGNEYLLASIAVVVIGGTSVAGGKANVPGVWGAALF
                     LVLLLTMLNTFGVSAGVRLVMTGLIIVLVITAAGGAKAER"
     gene            complement(6187..7170)
                     /locus_tag="VKU03_03695"
     CDS             complement(6187..7170)
                     /locus_tag="VKU03_03695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70395.1"
                     /translation="MTAPALAERPVSAEAGRLRLRRALRALLPAFSLVLVLAAIAYLN
                     SRAISYFGFNLMLNLAIPIALATLAQMFVIAGNDLDLSIGTFVGFVGCVAATWLHDQP
                     LLGVLILIGGVAAYAALGALVAWRDLPSIVVTLGMSFVWQGLAILLLPKPGGSAPHWL
                     LSLMGVKPPFVPFPIVAAIVIAVVAHIGLMRSSYGAILRGSGGNAAAIGRAGWSLFKA
                     KVTLFALAGFFGVLSGLALIGITTSADANIGNGYTLLSIAGVILGGGEFVGGRVSPIG
                     AVIGALTLALAASPLLTFMRIPPDWQVAANGAILIIVLAARVLISQRGAER"
     gene            complement(7172..8644)
                     /locus_tag="VKU03_03700"
     CDS             complement(7172..8644)
                     /locus_tag="VKU03_03700"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ70396.1"
                     /translation="MTGGAAPDLVRLDGVEKRFGAVRALDGVDFSVARGECVGLVGHN
                     GAGKSTLMNVLAGTIKADSGRLTINGETLRDYSPTRAKALGVRCVFQELSLCPNLSVA
                     ENARVFHPALKGFNWRRKAGGLILDKLDEIFPGHGVNPGDLVQDLSIGRRQMIETARA
                     FTVSDEPVRLVILDEPTSSLDAHTAGQLLSFVRRAVAQGTSCVLISHLLGEILNNSDR
                     IVVMRDGKEVASGRAASFDREKLMAAMGGAEERERKAAAIASTAPRGDAPIRVRARRP
                     GQKGETHIVAREGEIIGLAGLAGQGQTDLLLDIFAAASRPRADIEVTAPVALVAGDRQ
                     SDGIFPQWSILENIGIGSLARLREGPLISRRREEALANLWRDKIGIRTPDVANNILSL
                     SGGNQQKALFARALGSDARIVLMDDPMRGVDIGTKLEVYDLIREEARAGRTFLWYTTE
                     TEELQNCDRVYIFRNGAIVADLAHDALSEEKVIESSFEAG"
     gene            8856..10193
                     /locus_tag="VKU03_03705"
     CDS             8856..10193
                     /locus_tag="VKU03_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLJ70397.1"
                     /translation="MDSFTRRDTLALGAGALALTTPFAREALAAIPTADAAPLKLDIE
                     KGASLRVLRPSKFVATDDKIFNENTRKFSAATGVQVKVDYSAWEDLRPQTAVTANTGA
                     GPDVVLGWTDDPHLYSDKLIELSDVAEYLGKKYGGWYPLAERYGKKFGTNNWIAIPFG
                     GAGGPAVYRISWVKEAGFDTFPTDMDGFLKLCRALQKIGHPAGFTSGHAVGDGNNYCH
                     WLVWAMGGFMVDENQKVAINSKQTVEALKMAKALFETYIPGTQSWLDPSNNKALIAGD
                     IALTQNGVSLYYSIKNSDEPKIAAMAADLNNARMPIGPVGRGTEVALAINAMVFKHTK
                     YPNAAKAYLAFMMEADQYDKWLTGTFGYWAQPLKAYEASEVWTSDPKVAVYKDTMQNS
                     LWLGYKGPINEASAAVVADYIMVDMVASVETGSATPEEAAAEAERRAKRYYKA"
     gene            10346..11299
                     /locus_tag="VKU03_03710"
     CDS             10346..11299
                     /locus_tag="VKU03_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018641508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLJ70398.1"
                     /translation="MVSVVATPTPLTLRESWLTRLFDWKPFLVAVCLAPAVGLLIVFL
                     AYPLGLGFWLAFTDAKIGRPGQFIGLDNFVSLSRDPIFWLAVGNTLLYTAVATVGKFS
                     LGLWLALLLNEHVPFKSLIRAIVLVPWIVPTVLSAMAFWWIYDPQFSIISYFLKDVAH
                     LRRTNIDFLGTPWAARFSLIAANIWRGIPFVAICLLAGLQTISPSLYEAAMLDGATGW
                     QRFRYVTVPLLTPILSVVLTFSVLFTFSDFQLVYAITRGGPVNSTHLMATLAFQRGIP
                     GGQLGEGAAIAVAMIPFLIAATLLSYFGLGRRKWQQGGAND"
     gene            11292..>11675
                     /locus_tag="VKU03_03715"
     CDS             11292..>11675
                     /locus_tag="VKU03_03715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008138803.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLJ70399.1"
                     /translation="MTDTLAEAEIEAATGRVTDVPERLAWDSRMRRVVTVYIPLAIFV
                     IVLLFPFYWMAITAIKPNYEMEDYKRFNPFWVQSPTLENIRTLLFETDYPRWLLVTMS
                     VAVASTIISIFASVLAAYAIERLRFR"
BASE COUNT         2060 a         3814 c         3903 g         1898 t
ORIGIN      
        1 ggtccccctc ccccgtcttc gacgggggag gatggtctcc gccctactgg caatgacggt
       61 tttcgccgca attcgttgct tacatccgaa agcgccggcg ggcgcaggcc ggcagtttca
      121 gcccggttga tcagggcggg aggcgacgat gcgacagcga gcggagcatt gggtcacgat
      181 caggatcgcc gcggaggagg cggggcggtc ggaggagcgc cgcctgcagc aggaatacag
      241 cttgcacctc gaaagcgagg ggcggccgga cggcgcggtc atctattggc gcggcgagga
      301 agacggcggc cgcgtctatt atttctcccc gcaagccgcg gtgatcgccg gggccgcgct
      361 cgaacggttt tcgccgcggc cgatttcgcc ggacaatatg caggctttgc gcgcgcgagg
      421 gatgagcgtc gccaattggc ggcacagcta catgcatccg cggtgaggcg gcggccgggc
      481 gcttttaggt ccgcaagcgg cgcgccacgt cttccggcat cggccggctc ttcagccgcc
      541 cgtccgcctc ctcgacgacc cagacgcgcg tctcgcgcgc ctcgaccgcg agttctccct
      601 tgttgaagag cttgtgctcc acttcgaagc ttgaccgccc gaccgccgcc accgcggtga
      661 cgatctcgat ctcgtcgcca tagcgggcgg ggcggtggaa cgtcgctcgc gtgtcgacca
      721 gcgggatgcc ggcgacgcca tagatcgtcc gcatcgcggc cttttcgacg ccgagcgctt
      781 cgcgcaccag cagcgaggtc gaccagtcga aatattcgaa gaagcgcgga ttgaagacga
      841 tgccggccgg gtcgcaatgg ccccattcga cggtcaattt gcgggtgaac gacgccggca
      901 tgttttttct ctaaatgtga cgtaccccct ctcccgaagg gaaaggggca ggggcgaggg
      961 gaccggcgcg ccaggaaaaa gcgaagcggc gacgcgcgca ttcgaaccta tggcgtgaaa
     1021 tattcatgcg ttgtgcacgt cgcccctgtc cctttcccat cgtgagaggc ggcgctcacg
     1081 cccatcacgc cgccgcgaca ttctcgatca gcagcgcgat cccttgcccg ccgccgatgc
     1141 aggcgctggc gatcccgtag cgcaggcgcc ggcgctgcaa ctcgcgcgcc agggtcagcg
     1201 tcaagcgcac gccggtcgcg ccgagcggat ggccgatcgc gatcgcgccg ccattgacgt
     1261 tgagcttgtc ctcgtcgagg ccgagctcgc ggatgcaggc gaggacctgc gcgccgaacg
     1321 cctcgttgat ctcgatccgg tcgatccggt cgagccgcat cccgacgcgt tcgagcagcg
     1381 cccggatcgc cggcgcggga ccaatgccca tgatttccgg cggacagccg accgcgaccg
     1441 cgccgatgag ccgcgccaga ggcctctttc ccttcgcctt cagataggcg ccgctggcga
     1501 cgacggcgcc cgcggcgccg tcgaccacgc cggaggaatt gccgccggtc tggacgccgc
     1561 cgaaagccgg gcggattttg gcgagggtct cgcgcgtcgt cggccgcgga tgggtgtcct
     1621 cggcgacgcg ctcgatcttg cggtcgaggg cgacgccgcg gtccttgcgg ccgtcgcagc
     1681 ggaagatttc ggacgagacg gggacgatct cgtcggcgaa gaagccatcg gcgcgggcgc
     1741 tgagcgcgcg ctcgaaggag cgctcggcat aggcgtcgac ctccgtccgg ctgatctggt
     1801 atcgccgggc gaggttctcc gccgtcccgc ccatcgtcac gtcgcaggac gtatcgagca
     1861 aagcctccca gagaaaatcc ttgaactcga cctgtcccat ccggaagccg ccgcgatggg
     1921 tataggcggc gaccggattg cggctcatcg attcggttcc gacgcagagc gcggcttcga
     1981 cgcctttatg cgcgatcgcg tcgcaggcct gcgagacggc ctcgacgccg gtgccgcaaa
     2041 tgcgctggac gagatgggcc ggaacgtgct ccggcgcgcc gcaatagagg ccgatatggc
     2101 gcggcgtcat ataggcgtcg aagctcgcct gggccatcga gccggcgatg attgtaccaa
     2161 tgtcctcgcc ggaaacgccg ctcttctcca gagcggcgcg gccggccttg attccaagat
     2221 cgatcggcga gaccggcgcc agcggtccgt tgagatcggc aaaaggcgtg cgcgcgccgc
     2281 cggcgagata gacgtcgtcc caagtcgaga acacgccggg atgggtttcc gccattgttt
     2341 aggcctcgct ggcgcaaatg acgcgcgacg agaagggtcg cgcatagagt tcgtcaacga
     2401 gcgcggcgcg gcaactcaac accgcgcgct ggttgatcga gcccttatcg gtgatctcgc
     2461 cgcgatcgag cgacggcggc tcctcgagaa gcagcaagcg ggcgactcga ttggacgagc
     2521 cggtcgcggc ggccgcgaag gcgttcaggc ggcgctggat ttccgcaacc aaggcaggat
     2581 gggagaggat tgcgccaatc tcggctgcgc cgggctcgcc gcagaagctg cggcagccgt
     2641 cgatatcggg aaaggcgaga atggcgatgt cgtcgcgatt gaggccggcg accgccacgt
     2701 cgcggaagag cggcgcgaaa tgggcgatcg tccgggcgcg caacgggccg acgctgaccc
     2761 aggtcccggt cgccagcttg aaatcctcgg cgatgcgacc gtcgaacaga aagcccttgg
     2821 cgcggtccct ctcgtcgatg aatttcagcg cgtcgcccat ccggtaccag ccttcttcat
     2881 cgaaggcctt ggcggtcgcc tcggggtcac gccaatagcc ccgggtgacc gccggcccgc
     2941 gcacccgcgc ctcccatttg ccgtcgaccg gcgtcagctt caattcgacc ccggggaccg
     3001 gcaggccgag atggccggat ttggaatgtt cgggcgagca ggccaaggcg aaaggccccg
     3061 tctcggtcgc gccgaggccg gtcatcatcg gcacgcgctg gccgagcgtc tcgacggccg
     3121 ctgcgtcgat ctcgtcccag acatgctggg cgaggccggc gccggcgaag aagttcatat
     3181 tgagctgcga gaagaacgtt tcccgcagcg cgcgatcggc gcgcaggcgc ggcgccagct
     3241 cctcgaagcc cttcggcacg ttgaaataga cggtcgtggc gatctcgcgc agattgcgga
     3301 tcgtcgcctc gatccctttc ggcgtcggcc ggccctcgtc gatatagagc gagccgccat
     3361 tgtagaggac gaggccgaaa ttgtgattgc cgccgaatgt atggttccag ggcagccaat
     3421 cgaccaggac cggcggctgc tctttcaaga agcggaagta gttggcgatc atctcctggt
     3481 tggcggtcag catgagctgt gaattgatca cgcccttcgg cagagcggtc gagccggagg
     3541 tgaagaggat tttcgcgaca tccgcgccgc ggatcgccgc atgcgcccgc gcgacggccg
     3601 catcgtccgg cgcggcggcg aggagatcgg cgaaggcggt cgcgcgccgg ccgtcgggcg
     3661 gattgcgcgt gacgacgatt tccgcatcct ccggcgcgac cgccgccaag gcgcaaccat
     3721 agagcgcgcc gtcgcaggcg aagacgaggc cgggcgtcag cttgccggcg atatgggcga
     3781 gcttcgaaaa atcgccggcg atggtcgaat aggcgggcga gatcggcgcg taaggaacgc
     3841 cgacatgcag gcaggcgagc gccagcaggg catgctcgat gtcgttgccg gaaaggatca
     3901 tgaccggccg ctcggccgaa agcccgcgcc gcaacagctc ggcgccgatc gtccgcgccg
     3961 cgcgataggc ttcgccgtag gagaccttgc gccattcgtc ggcgccggcg cgctcggcca
     4021 tccacaggcg gtcgggcgcg acgctggcga agtgggcgag gcgctcggtg agcttttccg
     4081 gatgcgggcc gagcttctcg accggcgtca ccaggatcgc gccgacgccg cgcgtctcga
     4141 aacgcgctgc gttggagccg atcgagaaag gccgcagcgg cgcggtcctc ggatcgagct
     4201 tagggccgat cgcgctcgcg gcgcccgcca tgaccttccc tcctcgtttt ccgtcattgc
     4261 gagcgaagcg acgaagcaat ccattcgtca ggcgtcgccc tgtgaatgga ttgcttcgct
     4321 tcgctcgcaa tggcggtcaa tttggctcct tcggcgcgac cctgccggcg cgcttgtcga
     4381 gaaaagcccg catccgcgct ttcgcctccg catcgtcttg cgcgatcgcg ctcatcagcg
     4441 cttcgatcag gtagcccgcc gcgcggtcgc cgtcggcgat gcgcggcaaa gcctggatca
     4501 gcgcgaaatt ggtccgcccg gcattgccgg cgatcgcgcg cgcgagctcg accgccttgg
     4561 cgaggccttg gccgtcggcg acgacgtaat gcgagaagcc gaacggatag ccttcctcgg
     4621 cgcgatagac gcggccggtc agcatcatct ccatcatccg tgaggcgccg atcagccgcg
     4681 gcagccgcac cgagccgccg ccgccgacga aaatgccgcg ctggccctcg ggcaaggcgt
     4741 aaaaggccga gcgctcggcg acccgcacat gggtcgccgc ggcgagctcc agcccgccgc
     4801 cgaccaccgc gccatgcagc atcgagacga ccggcacgcg gccgaattcg atcgcctcga
     4861 aagcgcgatg ccagaggcgc gaatgctcga tcgcctggtt gaggtccttc tcggagagtt
     4921 cggaaaggtc gaggccggcc gagaaatgct cgccggcgcc gtgcagcacg gcggccctga
     4981 cgcctggcgg cagattgcgg aacgccgcct ccaaggcgag gatcgcctgg tcgttgagcg
     5041 cgttgcgctt ttgcggccgc tccaggcgta aaatcgcgat ctcgccctcg atccgcgtcg
     5101 agacgagttc tggcgcttga acggcggaag aaacgatcat gcgggcgttt cgctcgtgat
     5161 tgttattttg tataacaatc ggcgcgaggg tgtcaacggc tgtagccgcg gtcggcgacc
     5221 gcggctgcag cgccccgcct cagcgctccg ccttcgcgcc gccggccgcg gtgatcacca
     5281 gcacgatgat cagccctgtc atcaccagcc gcaccccggc gctgacgccg aacgtgttga
     5341 gcatggtcag gagcaggacc aggaacaggg cggcgcccca gacgccgggc acattggcct
     5401 tgccgcccgc caccgaggtt ccgccgatca ccacgacggc gatcgaggcg agaaggtact
     5461 cattgccgat gtcgacattg gcgccgcgga aatagccggc gagcagcgcg ccgtcgaggc
     5521 cgcccaggac gccgcacaaa gcgtaagtga ggaagcgcgt ccgtcccact ttgacgccgg
     5581 cgagccgcgc cgcgcggatg ttctgcccga tcgccaagac cgagcggccg tagatcgtgc
     5641 gctgcaggac gatcgccgcg gcgatggtga acagcacgac caggatcgcc aggatcggcg
     5701 cgccgaagac ctgggcgttg gcgaaattgg cgaagccggg cggcggcttg atctgcaagc
     5761 cgcggccgta cgagatgtcg atcgactgga tgacgaagct cgccgagagc gtggcgatga
     5821 tcggcgggat gcgcagcgcc cagatcagga ggtagttggc gacgccgatg ccgaggccgc
     5881 agcccaaagc cgcgaggatg ccgagcgcga ccagcgaatc gtcgccgttc atcactttca
     5941 tcgccaccgc gctggcgagg ccgatattgg ccggcagcga caggtcgaca ttgcccggcc
     6001 cgagcgtgat cacgaacatc tggccgacgc cgacgatcac cgtgaagacg gcgagcgaaa
     6061 aagccgcgtt gaccatgccg ctcgcgccat agccgccagt aaaggcgatc gaggcgagaa
     6121 agacgacgag cgcgccgagg aaggaccata gccagggctt ggcgaggagc cgggtcagag
     6181 ccgcattcat cgctcggcgc cccgctggct gatcaagacg cgcgcggcga ggacgatgat
     6241 caggatcgcg ccattggcgg cgacctgcca atcgggcgga atgcgcatga aggtgaggag
     6301 cggcgaggcg gcgagcgcca gagtcaacgc gccgatcacc gcgccgatcg gcgagacgcg
     6361 gccgccgacg aactcgccgc cgccgagaat gacgcccgcg atcgacagaa gcgtatagcc
     6421 gttgccgata ttggcgtcgg ccgaggtggt gatgccgatc aaggcgaggc ccgagaggac
     6481 gccgaaaaag ccggccagcg cgaacagcgt caccttggcc ttgaacagcg accagccagc
     6541 tcggccgatc gcggcggcgt tgccccctga gccgcgcagg atcgcgccat aggagctgcg
     6601 catcagcccg atatgggcga cgacggcgat aacgatggcg gcgacgatcg gaaagggtac
     6661 gaatggcggc ttgacgccca tgagcgacag gagccaatgc ggcgcgctgc cgcccggctt
     6721 gggcaaaagc aggatcgcca agccctgcca gacgaagctc atgccgagcg tcaccacgat
     6781 cgaaggcagg tcgcgccacg cgaccaaagc gccgagcgcg gcataggcgg cgacgccgcc
     6841 gatcaggatc agaacgccga gcagcggctg atcgtgcagc caggtggcgg cgacgcagcc
     6901 gacaaagccg acgaatgtgc cgatcgacag atcgagatcg ttgccggcga tcacgaacat
     6961 ctgggcgagc gtggcgagcg cgatcggaat cgccagattg agcatcaaat tgaagccgaa
     7021 atagctgatc gcgcgcgaat tgagataagc gatcgccgcc aggaccagca ccagcgaaaa
     7081 ggccggcagc aaggcgcgca aggcgcggcg gagcctcaat cggccggctt cggcggagac
     7141 ggggcgctca gcaagggcgg gggcggtcat gtcagcccgc ctcgaaagag gattcgatga
     7201 ccttctcctc gctcagcgcg tcgtgagcga gatcggcgac gatggcgccg ttgcggaaga
     7261 tatagacgcg atcgcaattc tgcagctctt cggtttccgt ggtgtaccat agaaaggtgc
     7321 ggccggcgcg agcctcttcg cgaatcaggt cgtagacctc cagcttggtg ccgatatcga
     7381 cgccgcgcat cggatcgtcc atgagcacga tgcgcgcgtc cgagccgagc gcccgggcga
     7441 acaaagcctt ctgctgattg ccgccggata gcgagagaat gttgttggcg acgtccggcg
     7501 tccggatgcc gatcttgtcg cgccaaagat tcgcgagcgc ctcttcgcgc cggcgcgaga
     7561 tcagcggccc ttcgcgcaag cgggcgagcg agccgatccc gatattctcg aggatcgacc
     7621 attgcggaaa gatgccgtcc gactggcggt cgccggcgac aagcgcgacc ggcgcggtca
     7681 cctcgatatc ggcccgcggc cggctcgccg ccgcgaaaat atcgagaagc aggtcggtct
     7741 ggccctggcc ggcaagaccg gccaggccga taatctcgcc ttcccgcgcc acgatgtgcg
     7801 tctcgccttt ttgtccaggc cgccgggcgc ggacccggat cggcgcgtcg ccgcgcggcg
     7861 cggtcgaggc gatcgctgcg gccttgcgct cgcgctcctc ggccccgccc atcgccgcca
     7921 tcaacttctc gcggtcgaac gaagcggcgc ggccgctcgc cacctccttg ccgtcgcgca
     7981 tgacgacgat gcggtcgctg ttgttcagaa tctcgccgag cagatgggag atcagcacgc
     8041 agctcgtccc ttgcgccacg gcccgcctga cgaagctcag caattggccg gcggtgtggg
     8101 cgtcgagcga ggaggtcggc tcgtcgagaa tgaccaggcg caccggctcg tcggacacgg
     8161 tgaaggcgcg cgccgtttcg atcatctggc gccggccgat cgagagatcc tgaacgagat
     8221 cccccgggtt gacgccgtgg ccgggaaaga tttcgtcgag cttgtcgagg atcagcccgc
     8281 cggccttcct gcgccagttg aaccctttga gcgcggggtg gaaaacccgc gcattctccg
     8341 cgacggagag attggggcag agcgacagct cctggaagac acaacgcacg ccgagcgcct
     8401 tggcccgggt cggcgaataa tcgcgcaagg tctcgccgtt gatcgtgagg cgcccggaat
     8461 cggccttgat cgtgccggcg agcacgttca tcagcgtcga cttgccggcg ccgttatggc
     8521 cgaccagacc gacgcattcg ccgcgagcga cgctgaaatc gacgccgtca agcgcgcgga
     8581 cggcgccgaa acgcttttcg acgccgtcca atctgaccag atcgggagca gctccgcctg
     8641 tcattccttc catcgggtcc ccgcatccgc cagccttttt ctaccgtcgt atcgctcata
     8701 gcaatatgct tcatcgtcag gtcgcgctgt cgccggccgt gggcggcgca gaggcctctg
     8761 aaaggtgcga cggattcgct ctggacttct cgatccaagg cgggtgatct gagcggcgac
     8821 cgcggccgaa acggccggga cgaggaggag acgcaatgga ttcgttcaca cgccgcgaca
     8881 cattggcgct gggcgcgggc gctttggcgc tgacgacgcc cttcgctcga gaggccctgg
     8941 ccgcgatccc gaccgccgat gccgcgcctt taaagctcga catcgaaaag ggcgccagcc
     9001 tgcgcgtctt gcggccgagc aagttcgtcg cgacggacga taagattttc aacgaaaaca
     9061 cccggaaatt cagcgcggcg accggcgtgc aggtgaaagt cgattattcc gcgtgggagg
     9121 atttgcggcc gcagaccgcg gtcaccgcca ataccggggc gggccccgac gtcgtccttg
     9181 gctggaccga cgatccgcat ctctattcgg acaagctgat cgagctcagc gacgtcgccg
     9241 aatatctcgg caagaaatat ggcggctggt acccgctcgc cgagcgctac ggcaagaaat
     9301 tcggcaccaa caattggatc gccatcccct tcggcggcgc cggcggccct gcggtctatc
     9361 gcatttcctg ggtcaaggag gccgggttcg acacgttccc gaccgatatg gacggcttcc
     9421 tgaagctctg ccgcgccctc cagaagatcg gccatcccgc cggcttcacc tcgggccatg
     9481 cggtcggcga cggcaacaat tattgccatt ggctggtttg ggcgatgggc ggctttatgg
     9541 tcgacgagaa ccagaaagtc gccatcaaca gcaagcagac ggtcgaggcc ctgaaaatgg
     9601 ccaaggcttt gttcgagacg tatatcccgg gcacccagtc ctggctcgat ccgtcgaata
     9661 ataaggcgct gatcgccggc gacatcgcgc tgacgcagaa cggcgtctcg ctctactatt
     9721 cgatcaagaa ttcagacgag ccgaaaatcg ctgcaatggc cgccgatctc aacaatgcgc
     9781 gcatgccgat cgggccggtc ggacgcggca ccgaagtggc gctcgccatc aacgccatgg
     9841 tgttcaagca cacaaaatac cccaacgccg ccaaggccta tctcgctttc atgatggagg
     9901 cggatcagta cgacaagtgg ctcaccggca cgttcggcta ttgggcgcag ccgctgaagg
     9961 cctacgaagc gagcgaagtc tggacgagcg atccgaaagt cgccgtctac aaggatacga
    10021 tgcagaactc gctctggctc ggctataagg ggccgatcaa tgaggcctcg gccgcggtcg
    10081 tcgcggacta catcatggtc gacatggtgg cctcggtcga gaccggctcg gcgacgcccg
    10141 aagaggcggc ggcggaggcc gaacggcgcg ccaagcgtta ctataaggct taacgagacc
    10201 gatataggcc gccattgcga gcgaagccaa gcaatccact cgtaaggctc agctctacga
    10261 ttggagtgct tcgctacgct cgcaatgacg gcgtccaaag atggcggcag gctccggcgg
    10321 agccgcggcg cggaaggaga agctcatggt cagcgtcgtc gcgacgccga cgccgctcac
    10381 gcttcgcgaa agctggctga cgcggctctt cgattggaaa ccgttcttgg tcgccgtctg
    10441 cctcgccccg gcggttgggc tgctgatcgt ctttctcgcc tatccgctcg gcctcggctt
    10501 ctggctcgct ttcaccgacg cgaagatcgg ccgtcccggc cagttcatcg ggctcgacaa
    10561 tttcgtctcg ctgagccgcg atccgatttt ctggctcgcg gttggcaaca cgctgctcta
    10621 tacggcggtc gcgacggtcg gcaaattctc gctcggcctg tggctggcgc tgctgctcaa
    10681 cgagcatgtg ccgttcaaat cgctcatccg cgccatcgtc ctggtgccgt ggatcgtgcc
    10741 gaccgtgctt tcggccatgg cgttttggtg gatctacgat ccgcaattct cgatcatttc
    10801 ctatttcctg aaggatgtgg cgcatctgcg ccgaaccaat atcgacttcc tcggcacgcc
    10861 ctgggcggcg cgcttctcgc tgatcgccgc caatatctgg cgcggcattc ctttcgtcgc
    10921 gatctgcttg ctcgccggcc tgcaaaccat ttcgccctcg ctctacgagg cggcgatgct
    10981 cgacggcgcg accggctggc agcggtttcg ctacgtcacc gttccgctgc tgacgccgat
    11041 tctctcggtc gtgctgacct tttcggtcct cttcaccttc agcgatttcc agctcgtcta
    11101 tgcgatcacc cgcggcggcc cggtcaattc gacccatctc atggcgactt tggccttcca
    11161 gcgcggcatt ccgggcggcc agctcggcga gggcgcggcg atcgcggtgg cgatgatccc
    11221 gttcctgatc gccgccacgc tgctcagcta tttcgggctc ggccgacgca agtggcagca
    11281 gggcggcgcc aatgactgac acgcttgccg aggcggagat cgaagcggca accggacggg
    11341 taaccgacgt tcccgagcgc ctggcctggg attcacggat gcgccgggtg gtgaccgtct
    11401 atatcccgct cgccatcttc gtgatcgtcc tgctgttccc gttctattgg atggcgatca
    11461 ccgccatcaa accgaactac gagatggagg actacaagcg gttcaaccca ttctgggtac
    11521 agtcaccgac gctcgagaat atccgcacgc tgctgttcga gaccgactat ccgcgctggc
    11581 tgctggtgac gatgtcggta gcggtcgcgt ccaccatcat ttccatcttt gcaagcgtgc
    11641 tcgcagcgta cgcgatcgag cgcttgcgct tccgc
//
LOCUS       DATGJW010000126         2150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1267337, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000126 DATGJW010000000
VERSION     DATGJW010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2150
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1267337"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(246..1202)
                     /locus_tag="VKU03_03720"
     CDS             complement(246..1202)
                     /locus_tag="VKU03_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008533434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HLJ70400.1"
                     /translation="MAKKLDGDEGRLDDAARAGWLYYVAGNTQDEIARKLGISRQSAQ
                     RLVSLAISERLIKVRLDHPIARCMELAAALTTRYDLRFCEISPTDSASASTTLGIAQL
                     AAAEFERWLRRDEPAIIGIGTGRTMRAVADQLPRMECPQHKLVALVGTTKIDGSASFY
                     DVVIRVSDTVRAPHYPMPLPVIARSVEERELLTGLASVKSVLALAERADVSFVGVGAI
                     QESAAIVLDGIISREEAEALRAGGAVGEITGWSFDARGRVLDNAHNSKVASAPLRPSP
                     NRLMIGVAMGASRRAAIRGALAGRLITGLVTDEATAEHLLYT"
     gene            complement(1355..>2150)
                     /locus_tag="VKU03_03725"
     CDS             complement(1355..>2150)
                     /locus_tag="VKU03_03725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAD family phosphatase"
                     /protein_id="HLJ70401.1"
                     /translation="ACRPAQRFEARGKRARCARKREAGAMAWPEVVIFDCDGVLVDSE
                     VIALAETRLALGEAGLHLSDEDVFDRFLGLSVEAIARSAEKELGETLPASFPGDLTRR
                     ILASFENELKGVAGIRQALAGLGARVCVASSSSPERIRRSLAAVGYANLFEPNIFSAR
                     AVERGKPDPDLFLFAARRMGAPPEACLVIEDSVPGVQAAAAARMQVFGFTGGSHISGP
                     AQGERLSAAGAALVFDDMTELPGIIRLRRESLLPASRGEGGREAAG"
BASE COUNT          361 a          722 c          720 g          347 t
ORIGIN      
        1 cgtcagcgtg gtctccgcat gggcggcgag cgagaagacg gccagagcgc aggccccgag
       61 caggctcttc acgagaacct tcatggtgtt tcctccatcg gaactggttg agcaaatgct
      121 tgttgctcgg gcgaatactc atgcaagcgg ggccgcagtg tcaacccgga atcgtcgttg
      181 cgccgcagct atcgcggtgc accatcacga taacttgctg tatttagtaa tgtttataaa
      241 tttgctcagg tgtagagcag gtgctcggct gtggcttcgt cggtgaccag gccggtgatg
      301 agcctgccgg cgagcgcgcc gcggatcgcg gcgcggcgcg aggcgcccat cgccactccg
      361 atcatcagcc gattggggct gggccgcagc ggcgcgctgg cgactttcga attgtgcgcg
      421 ttgtcgagca cgcggccgcg cgcatcgaag ctccagccgg tgatctcgcc gaccgcgccg
      481 ccggcgcgca gcgcctcggc ctcctcgcgc gaaatgatgc cgtcaaggac aatcgcggcg
      541 ctttcctgga tcgcgccaac gccgacaaag ctgacatcgg cgcgctcggc gagggcgagc
      601 acgctcttga ccgaggcgag ccctgtcagc aattcgcgct cttcaaccga gcgggcgatc
      661 accggcagcg gcatcggata gtgtggggcg cgcacggtgt ccgacacgcg aatgacgacg
      721 tcgtagaagg aggcggagcc gtcgatcttg gtcgttccga ccaaagccac cagcttgtgc
      781 tgcggacatt ccatgcgcgg caattggtcg gcgacggcgc gcatggtgcg gccggtgccg
      841 atgccgatga tcgccggctc gtcgcggcgc agccagcgct cgaactcagc cgcggcgagc
      901 tgcgcgatgc cgagcgtcgt cgaggccgag gcgctgtcgg tcggcgaaat ttcgcagaag
      961 cgcagatcgt agcgagtcgt cagcgccgcg gcgagctcca tgcagcgggc gatcggatga
     1021 tccagacgta ctttgatgag gcgttcgctg atcgcgagcg agaccaggcg ctgcgccgat
     1081 tggcgcgaaa tgccgagctt gcgcgcgatc tcgtcctgcg tgttgccggc gacatagtac
     1141 aaccagccgg cccgcgccgc atcgtcgagc cgcccctcgt ccccgtccag cttcttggcc
     1201 attgccgaat aagttccttc acgtaccaag gcgctgcatt tccttctccc cgctctgcgg
     1261 gaggaggcgg cgcgaagcgc cggatgaggg gcggcgcggg actctgaagg ttaagcgctt
     1321 caccgccgct catcccaaag cgcggcgcag cccctcatcc ggccgcttcg cggccacctt
     1381 ctccccgcga agcggggaga agggattcgc gcctcagtct tataattccc ggcaactccg
     1441 tcatatcgtc gaaaaccaaa gccgcgccgg cggcgctgag gcgctcgccc tgggcgggac
     1501 cggaaatgtg gctgccgccg gtaaagccga agacttgcat gcgcgcggcc gccgccgcct
     1561 gcacgccggg aacgctgtcc tcgatgacga ggcaggcttc cggcggcgcg cccatccggc
     1621 gcgccgcaaa aagaaaaagg tcggggtcgg gcttgccgcg ctcgaccgcg cgcgccgaga
     1681 aaatgttcgg ctcgaacaga ttggcgtagc cgaccgccgc cagagagcgg cggatgcgct
     1741 cgggcgagct cgacgaggcg acgcagaccc gagcgccgag ccccgccagc gcctggcgga
     1801 tgccggcgac gcctttgagc tcgttttcga aactggcgag gatgcgccgc gtcaggtcgc
     1861 ccggaaagct cgccggcaaa gtctcgccca gctccttttc cgcgctgcgc gcgatcgcct
     1921 cgacgctgag accgaggaaa cggtcgaaca cgtcctcgtc gctcaaatgc aagccggctt
     1981 cgcccagagc cagccgcgtc tcggcgagcg cgatcacctc gctgtcgacc agcacgccgt
     2041 cgcaatcgaa aatgacgacc tcaggccagg ccatggcgcc tgcttcgcgt ttcctcgcgc
     2101 aacgggcgcg cttgccgcgc gcttcgaacc tttgcgccgg ccggcaggct
//
LOCUS       DATGJW010000127         3536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_748632, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000127 DATGJW010000000
VERSION     DATGJW010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3536
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_748632"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..796)
                     /locus_tag="VKU03_03730"
     CDS             complement(<1..796)
                     /locus_tag="VKU03_03730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ70402.1"
                     /translation="MSAADFFPGFATRMIAAPAGSIFARIGGGGPPLLLLHGFPQTHV
                     EWRLIAPRLAEKFTLVLMDLRGYGGSSVPPSENGTAYSKREMAKDAVAVMAALGFERF
                     GVVGHDRGGRVAYRLALDAPERVDKLAVLDIIPTGEMWRSMDAARAMQTYHWLFLAQP
                     APMPENLIRGAAIAWLKHTLASWTGDKSLAAFGDALDAYCAAFDRQERIHAFCEDYRA
                     GATIDRRLDDEDHAAGRRIAAPLLALWGAKGIPSEGDDPLTIWRRWA"
     gene            complement(793..2115)
                     /gene="purD"
                     /locus_tag="VKU03_03735"
     CDS             complement(793..2115)
                     /gene="purD"
                     /locus_tag="VKU03_03735"
                     /EC_number="6.3.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892856.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004637 - phosphoribosylamine-glycine
                     ligase activity [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylamine--glycine ligase"
                     /protein_id="HLJ70403.1"
                     /translation="MNVLIIGSGGREHALALALKKSPRLDRLFVAPGNAGAAEVAEQI
                     AIAIGDHRAVIDFCRKQAVGLVVVGPEAPLVAGLADDLAAAEILCFGPSKAAARLEGS
                     KGFAKDFCREYGIPTAAYRRFRALEPALAYLREKGAPIVIKADGLASGKGVVVAASLA
                     EAEAALRSMFDGAFGAAGAEVVIEEVLEGEEVSFFALSDGERAVAFASAQDHKRVGDG
                     DAGPNTGGMGAYSPAPVMTDAMSARVMREIVLPTIAGMRARGAPFRGALFAGLMIGAD
                     GPKLIEYNVRFGDPEAEVVLARFDGDLLALLLGCAAGALPETSPRFLDRAALTVVIAA
                     QGYPGAPRKGTEIRGIERAGAMPGVTIHHAGTRRDGGRILADGGRVLAVTALGGSVGE
                     AQSRAYAAVDAVDWPEGFCRRDIGWRAVARERSQEARGAEAPVAARRA"
     gene            2285..2803
                     /locus_tag="VKU03_03740"
     CDS             2285..2803
                     /locus_tag="VKU03_03740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CreA family protein"
                     /protein_id="HLJ70404.1"
                     /translation="MKWRFLFPLAALAAVFFASAALADSGGPDLIFRKSTTFKLLTPN
                     DKLATYAIDDPLVDGVACYFTVSEKGGVSGALGLAEQTSDVSLACRQYGPIKIKDKFE
                     QGDVVFRERRSLFFKKMQIVRGCDAKRNVLIYMAYSDKLVEGSPKNSTSSVPIAPWGG
                     GAEATKCGDFLR"
     gene            complement(2813..3115)
                     /locus_tag="VKU03_03745"
     CDS             complement(2813..3115)
                     /locus_tag="VKU03_03745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70405.1"
                     /translation="MRRLASLPAFLLVLLLVGPAAAGPDAASRNFIIAASEGYGVQDC
                     LGGEGGCGQVVADAWCEAHGEGPALAFGKTDDVTGAIASRPAQNIPAGAYFITCGR"
BASE COUNT          584 a         1198 c         1200 g          554 t
ORIGIN      
        1 ccgcccagcg ccgccagatt gtcagcggat cgtcgccctc gctcggaatg cccttggcgc
       61 cccagagcgc cagcaagggc gcggcgatgc ggcggccggc ggcgtggtcc tcgtcgtcga
      121 ggcgccggtc gatcgtcgcg ccggcgcggt agtcttcgca aaaggcgtga atgcgctctt
      181 gccgatcgaa ggcggcgcaa taggcgtcga gcgcgtcgcc gaaagcggcg aggcttttgt
      241 cgccggtcca actggccaaa gtgtgcttga gccaggcgat cgccgcgccg cgaatcaggt
      301 tttccggcat cggcgccggc tgggcgagaa acagccaatg ataggtctgc atggcgcgcg
      361 ccgcgtccat gcttcgccac atctcgccgg tcggaatgat gtcgaggaca gcgagcttgt
      421 cgacccgctc gggcgcgtcc aaggcgaggc gataggcgac gcggccgccg cgatcgtggc
      481 cgacgacgcc gaaccgctcg aagccgagcg ccgccatgac ggcgacggcg tccttggcca
      541 tttcccgctt cgaataggcg gtgccgttct cgctcggcgg gacggacgag ccgccatagc
      601 cgcgcagatc catcaggacg agagtgaatt tctcggcgag gcgcggcgcg atcagccgcc
      661 attcgacatg cgtctgcgga aagccatgca gcagcaggag cggcggcccg ccgccgccga
      721 tccgcgcgaa gatcgagccg gccggcgcgg cgatcatgcg cgttgcaaag ccggggaaga
      781 aatccgccgc gctcatgcgc gcctcgccgc gacgggcgct tcagcgccgc gcgcctcctg
      841 ggatcgctcc cgcgcgaccg cccgccagcc gatgtcgcgg cggcagaacc cctccggcca
      901 atccaccgcg tcgacggcgg cataggcgcg gctttgcgcc tcgccgaccg agccgccaag
      961 ggcggtgacg gccagaacgc ggccgccatc ggcgagaatg cgcccgccgt cgcgccgcgt
     1021 cccggcatgg tggatcgtca cgccgggcat ggcgccggct cgctcgatgc cgcggatttc
     1081 cgttcctttg cgtggagcgc cgggatagcc ctgagcggcg atgacgaccg tcagcgcggc
     1141 tcgatccaga aaacgcggcg acgtttcggg cagcgcgccg gcggcgcagc cgagcagcag
     1201 cgccaacaga tcgccgtcga agcgcgccag cacgacctcg gcttcgggat cgccgaaccg
     1261 gacattgtat tcgatgagtt tcggcccgtc ggcgccgatc atcagcccgg cgaagagcgc
     1321 gccgcgaaac ggcgcgccgc gcgcccgcat gccggcgatc gtcggcaaga cgatctcgcg
     1381 catgacgcgc gccgacatgg cgtcggtcat gaccggggcg ggcgagtagg cgcccatgcc
     1441 gccggtattc ggcccggcgt cgccgtcgcc gacccgctta tgatcctgcg cgctggcgaa
     1501 agcgacggcg cgctcgccgt ccgaaagcgc gaagaacgac acttcctcgc cttccaagac
     1561 ctcctcgatg acgacctccg cgcccgccgc gccgaaggcg ccatcgaaca tcgaccgaag
     1621 cgccgcctcc gcctcggcca agctcgcggc gacgaccacg cctttgccgg aggccagccc
     1681 gtcggccttg atcacgatcg gcgcgccctt ctcgcgcaaa taggcgagcg ccggttcgag
     1741 cgcgcgaaag cgccgatagg ccgcagtcgg gatgccgtat tcgcggcaga aatccttggc
     1801 gaagcctttc gagccttcga gccgagccgc ggctttgctc ggcccgaagc agaggatttc
     1861 cgccgcggcg aggtcgtcgg ccaggccggc gacgagcggc gcctccggcc cgacgacgac
     1921 gaggccgacc gcctgcttgc ggcaaaagtc gatgacggcg cgatggtcgc cgatcgcgat
     1981 cgcgatctgc tcggcgacct cggccgcgcc ggcattgccg ggcgcgacga acaagcgatc
     2041 gaggcgaggc gatttcttta gcgccaaagc gagcgcatgc tcccggccgc ccgagccgat
     2101 gatcaagaca ttcatgcggc ctcgcatcgt ttcggcgggt cgtcccgcct tgtttaggcg
     2161 agggggccgc tgcgggcaat gcggcgtggc gggcccgccg cgccgcttgc gccgcgccgc
     2221 gcaagaccta tctcttggcg gctttcagcc ggccatccgg cttaacacag cggagccgag
     2281 cctgatgaaa tggcgctttc tctttcccct cgcggcgctg gccgcggtct tctttgcgag
     2341 cgcggctctg gccgattccg gcgggccgga cctgattttc cgcaagtcga caacgttcaa
     2401 gctgctgacg ccgaacgaca agctcgccac ttacgcgatc gacgacccgc tggtcgacgg
     2461 cgtcgcgtgc tatttcaccg tgtcggagaa gggcggcgtc tccggcgcgc tcgggctcgc
     2521 cgagcagaca tcggacgtgt cgctcgcctg tcggcaatat gggccgatca agatcaagga
     2581 caagttcgag cagggcgatg tcgtcttccg cgagcggcga tcgcttttct tcaagaagat
     2641 gcagatcgtg cgcggctgcg acgccaaacg caacgtgctg atctatatgg cctattcgga
     2701 taagctggtc gagggatcgc cgaagaattc gacttcctcg gtgccgatcg ccccctgggg
     2761 cggcggcgcc gaggcgacca aatgcggcga cttcctgcgc tgagcgcttc ggctaccggc
     2821 cgcaggtgat gaaataggcg ccggcgggga tgttctgggc cggacggctg gcgatcgcgc
     2881 cggtcacgtc gtcggtcttg ccgaaagcca gagccggccc ctcgccatgc gcctcgcacc
     2941 aggcgtcggc gaccacctgg ccgcagccgc cctcgccgcc gaggcaatcc tgcacgccat
     3001 aaccctccga ggcggcgatg atgaaattgc gcgacgcggc gtccggtccg gccgcggcgg
     3061 ggccgacgag aagaaggacg agaaggaagg cgggcaagga cgcgagacgg cgcatgcgct
     3121 cagcttttcg gcaagcggtg ggacggacca gaggcgaggc cttaaggatg agtcggcctt
     3181 gtgtatttta agttaacggg ctgtcattcc cgtgaaccgg aatccagaga gccgccatgc
     3241 ttaggggttg ccctcagtct ggattcccgc ttttcgcggg aatgacatgc gccttgactt
     3301 cccctgcggc ctgcgggaaa gaaagatcat gtcgggacaa tcctgcattt gccggattat
     3361 tattattcgc cgctagtcta gaactggctg gcgcccgtct tttttcactt cccatctgaa
     3421 aagcgacgca atgccggcca ggcgcattag cgcgcggtta tgtcgagacg gacatgtcgg
     3481 aacctttgcg gctctacaac acgctgacgc ggtcgaaaga ggttttcgaa cccatc
//
LOCUS       DATGJW010000128         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_127610, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000128 DATGJW010000000
VERSION     DATGJW010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_127610"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..852
                     /locus_tag="VKU03_03750"
     CDS             <1..852
                     /locus_tag="VKU03_03750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HLJ70406.1"
                     /translation="DRVVIGVEDEEGRRVMSDIYRPLDLNQGALLFTSRRTSELVKYA
                     ANAYLAMKVAFINEISDLCEQVGGDVQDVARGVGLDNRIGYKFLNAGPGFGGSCFPKD
                     TLALMKIAKEAGSPLRLVETLVEANENRKLAMARKIVKACGGSVKGKTIAVLGLTFKP
                     NTDDMRSAPSLTIVPALQAEGARVVAFDPEGEKSARELLPGVEFADGPYSCLQGADAL
                     VIITEWDAFRALDLARVKAALRQPIVVDLRNIYRREEMTALGFHYESLGRGRKSAVGS
                     RRVGSRE"
     gene            933..2372
                     /gene="rfaE1"
                     /locus_tag="VKU03_03755"
     CDS             933..2372
                     /gene="rfaE1"
                     /locus_tag="VKU03_03755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0009244 - lipopolysaccharide core region
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-glycero-beta-D-manno-heptose-7-phosphate
                     kinase"
                     /protein_id="HLJ70407.1"
                     /translation="MLPALAQSSLLVVGDAMLDVYVDGAVERVSPEAPAPVLRHSGDR
                     HLAGGAANVAANIARLGAAVRLIAPVGADDEAARLAGLLEQAGIDFDAIVDSDRPTTV
                     KTRVMSGAHQLLRVDRETTRPSSAAIEDEILARIAAALPNVQALVLSDYAKGALTDRV
                     LAGAIAAARARDKPIFVDPKRRDFAAYAGATLITPNRGELALATGLSCESDEEIESAA
                     RKLIAATGAAILVTRSDKGMSYFAEGAAALHMPTKARQVFDVSGAGDTVIAAVACCRT
                     GGLPIEQTMRIANLAAGIVVGKAGAATASWEEIRDADEHDLPPSLRKGALANRDEAAA
                     IREHWRREGLNVGFTNGCFDLLHPGHIETLRYAARHCDRLIVGLNSDASTRRLKGPSR
                     PIQDEEARAIVLGAIHCVDLVVLFAEDTPQELIAALRPDTLVKGADYAESEIVGAEIV
                     KAAGGRVLRAPLLDGLSTTSLIARKAVGA"
BASE COUNT          451 a          916 c          901 g          459 t
ORIGIN      
        1 gaccgcgtcg tgatcggcgt cgaggacgag gagggccggc gggtgatgag cgacatctat
       61 cggccgctcg atctcaacca aggcgcgctg ctcttcacca gccggcgcac gtccgaactc
      121 gtcaaatacg cggccaacgc ctatctggcg atgaaagtcg ccttcatcaa cgagatcagc
      181 gacctttgcg agcaggtcgg cggcgacgtg caggatgtcg cgcgcggcgt cggcctcgac
      241 aaccgcatcg gctacaagtt cctcaatgcc gggcccggct tcggcggctc gtgctttccc
      301 aaagatacgc tcgccttgat gaagatcgcc aaggaggcgg gctcgccttt gcggctggtc
      361 gagaccctgg tcgaggccaa cgagaaccgc aagctcgcca tggcgcgcaa gatcgtcaaa
      421 gcctgcggcg gctcggtgaa gggcaagacc atcgccgtgc tcggcctcac cttcaagccg
      481 aacaccgacg acatgcgctc ggcgcccagc ctgaccatcg tccccgcttt gcaggccgaa
      541 ggcgcccggg tcgtcgcctt cgatccggaa ggcgaaaaat cggcgaggga gctgctgccc
      601 ggcgtcgaat tcgccgacgg gccctattcc tgcctccagg gcgccgacgc cctggtcatc
      661 atcaccgaat gggacgcgtt ccgcgccctc gatctcgccc gggtcaaggc ggccctacgc
      721 cagccgattg tcgtcgacct gcgcaacatc tatcgccgcg aggagatgac ggcgttgggc
      781 tttcattatg agagcctggg gcggggaagg aaatcggcag tcggcagtcg gcgggtcggc
      841 agtcgggagt agcggccaac cggctgccga ctgccgatct gccgactgcc gtctccttcc
      901 cgctttccat tccatcctct atccttcccg gcatgctgcc cgctctcgcc caatcgtccc
      961 tgctcgtcgt cggcgacgcg atgctcgacg tctatgtcga tggcgcggtg gagcgcgtct
     1021 cgcccgaggc gccggcgccg gttttgcggc attcgggcga tcgccatctc gccggcggcg
     1081 cggccaatgt ggcggccaat atcgcaaggc tcggcgccgc ggttcgcttg atcgcgccgg
     1141 tcggcgcgga cgacgaggcg gcgcgcctcg ccggtctgct cgaacaagcc ggcatcgatt
     1201 tcgacgcgat tgtcgattca gaccgtccga cgacggtcaa gactcgcgtg atgagcggcg
     1261 ctcatcaatt gctgcgcgtc gatcgcgaaa cgacccggcc gagttcggcc gcgatcgaag
     1321 atgaaattct ggcgcgcatc gcggctgcgc tgcccaacgt tcaggccttg gttctctccg
     1381 actacgccaa gggcgccttg accgaccggg tgcttgccgg cgcgatcgcg gcggcgcggg
     1441 cccgcgacaa gccgatcttc gtcgatccga agcgccgcga tttcgccgct tacgccggcg
     1501 cgacgctgat cacgcccaat cgcggcgaac tcgctttggc gacgggcctc tcgtgcgaga
     1561 gcgatgagga gatcgagagc gccgcgcgca agctgatcgc ggcgaccggc gcggcgatcc
     1621 tggtgacgcg ctccgacaaa ggcatgtcct atttcgccga gggcgcggcg gcgctccaca
     1681 tgccgaccaa ggcgcggcag gtcttcgacg tctccggcgc cggcgatacg gttatcgcgg
     1741 cggtcgcgtg ttgccggacc ggcggcttgc cgatcgagca gacgatgcgc atcgccaatc
     1801 tggcggcggg aatcgtcgtc ggcaaagccg gcgcggcgac ggcgagctgg gaggagatca
     1861 gggacgccga cgagcatgac ctgccgccgt cgctgcgcaa gggcgcgttg gcgaaccggg
     1921 acgaagcggc ggcgatccgc gagcattggc ggcgcgaagg cctgaatgtc ggcttcacca
     1981 atggctgttt cgaccttctt catcccggcc atatcgagac tttgcgctat gcggcgcgcc
     2041 attgcgaccg gctgatcgtc ggcttgaaca gcgacgcctc gacgcggcgc ctgaaagggc
     2101 cgtcgcggcc gatccaggac gaagaagcgc gggcgattgt gctcggcgcc atccattgcg
     2161 tcgacctcgt cgtcctgttc gccgaggata cgccgcaaga gctcatcgcg gctttgcgcc
     2221 ccgacacgct ggtcaaaggc gccgactacg ccgagagcga gatcgtcggc gccgagatcg
     2281 tcaaagccgc cggcggccgc gtcctgcgcg ccccgctgct cgacggcctt tcgacgacca
     2341 gcctgatcgc gcgcaaagcc gtcggggcgt aactcccctt ctccccgcaa ggcgcagggc
     2401 tgtccgggga aagtaagagg cgagtcgcat cgtcgtcgac gaagtttcgt gaaataacac
     2461 gatttcgaga tttcctcgtt gagtttccgg cgccgagact cagaaataaa catgcggacc
     2521 tcggggctgc tcacaaactc ttgcgagcag tacggatgca acggtgacgc aaatccccta
     2581 caccgcgctg ccatcgccgc ggggcggcgt caatcccaag ccggctttcg ccggtcgctt
     2641 cgcttcgctg cgctgcgctg cgcgaggatt gaccccgccc cgcgtcgatg gcctgtttgc
     2701 caccaagagc tttggtgagg aaactgg
//
LOCUS       DATGJW010000129         4239 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_645895, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000129 DATGJW010000000
VERSION     DATGJW010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4239)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4239)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4239
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_645895"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /gene="gatB"
                     /locus_tag="VKU03_03760"
     CDS             <1..1161
                     /gene="gatB"
                     /locus_tag="VKU03_03760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HLJ70408.1"
                     /translation="YKSPIVGEGEVLVDLSHDRSIKVGIERLHLEQDAGKSLHDQSPT
                     MSFVDLNRSGVALMEIVSKPDMRSADEAKAYVTKLRAILRYIGSCDGDMEKGNLRADV
                     NVSVRRPGEPFNTRCEIKNVNSIRFIGQAIEAEARRQIALIEDGGKVEQETRLFDPGR
                     GETRSMRSKEEAHDYRYFPDPDLLPLELDEAAIADLAAHLPELPDAKKARFIADYGLS
                     SYDAGVLVAERETADYFEAAVAHGGRKRDAKAVANWIMGDLAAFANSQSRTIAETHVK
                     PAQIADLVDLIEAQTISGKIAKDVLAIIFAEEKDAAPSAIVEARGLRQVTDTGAIEAA
                     VDRIIAANPDKVAQAKAKPTMLGWFVGQTMKATGGKANPQAVNDALKRKLGL"
     gene            1592..1978
                     /locus_tag="VKU03_03765"
     CDS             1592..1978
                     /locus_tag="VKU03_03765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70409.1"
                     /translation="MKRCARVMRDLLSQGTQLALLASDVCAQQMRGQWNLRGTNQMAK
                     AAAKKSSRKTAAKKTARKGAAKKTARKGARKGAAKKGARKGAAKKGARKGAAKKKAAK
                     KKAPAKRKAQKKPEPAPTPPPMAEPT"
     gene            2204..2297
                     /locus_tag="VKU03_03770"
     tRNA            2204..2297
                     /locus_tag="VKU03_03770"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2238..2240,aa:Ser,seq:gct)
     gene            complement(2462..2854)
                     /locus_tag="VKU03_03775"
     CDS             complement(2462..2854)
                     /locus_tag="VKU03_03775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ70410.1"
                     /translation="MIVVDASALLETLLQTPAAKTVSERLFNSRQSLYAPHLIDIEVA
                     QIIRRLAAKGEIETERGRAALVDLADFPLTRHPHHFLLPRIWALRNNVTAYDAAYVAL
                     AEALDAPLLTRDRRLAAAPGHHAQIEVF"
     gene            complement(3141..3356)
                     /locus_tag="VKU03_03780"
     CDS             complement(3141..3356)
                     /locus_tag="VKU03_03780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70411.1"
                     /translation="MLLGAILLGASTAAYADMGTPEQRAACAPDVRKFCHKVKESDGA
                     DAYLQCLELNRSKLSARCVGMLQSYGK"
     gene            3606..>4239
                     /locus_tag="VKU03_03785"
     CDS             3606..>4239
                     /locus_tag="VKU03_03785"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF020039.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLJ70412.1"
                     /translation="MRGQAEIAVKAGNALGEGIVWSPAHGEVQWTDIHGQAFWSFDPA
                     TGAARSIALPERLACFAPLGGARILAGFAGGLARFDLVSGARQDIAAIEPEEGRTRLN
                     DGKLDRQGRLVFGTMDESKDAQPLGRVWSFDGERPRVLFGGVRISNSIAFSPDGRRMY
                     FADTPQRVIWRFDYDSASGDVANRQVFVEVAPDHGYPDGSAVDADGCLWNA"
BASE COUNT          804 a         1349 c         1394 g          692 t
ORIGIN      
        1 tataagagcc cgatcgtcgg cgagggcgag gtgctggtcg atctctcgca tgacaggtcg
       61 atcaaggtcg gcatcgagcg gctgcatctc gagcaggacg ccggcaagtc cctgcacgac
      121 cagtcgccga cgatgagctt cgtcgacctc aaccgctccg gggtcgcgct catggagatc
      181 gtctccaagc ccgacatgcg ctcggccgac gaggccaagg cctatgtgac caagctgcgc
      241 gcgatcctcc gctatatcgg ctcctgtgac ggcgacatgg agaaggggaa cttgcgggcc
      301 gacgtgaatg tctccgtgcg ccggcccggc gagccgttca acacgcgctg cgagatcaag
      361 aacgtcaatt cgatccgctt tatcggtcag gcgatcgagg ccgaggcgcg ccgccagatc
      421 gcgctcatcg aggacggcgg caaggtggag caggagacgc ggctcttcga tccgggccgc
      481 ggcgagacgc ggtcgatgcg ctcgaaggaa gaggcgcacg attaccgcta ttttcccgat
      541 cccgatttgc tgccgttgga gctcgacgag gccgcgatcg ccgatctcgc tgcgcacttg
      601 cccgaactgc ccgacgccaa gaaggcgcgc ttcatcgccg attacggcct ctcctcctac
      661 gacgcgggcg tgctcgtcgc ggagcgcgag acggccgact atttcgaagc ggcggtcgct
      721 cacggcggcc gcaagcgcga tgcgaaagct gtcgccaact ggatcatggg cgacctcgcc
      781 gccttcgcca acagccagtc gcgcacgatc gccgaaacgc atgtgaagcc ggcccagatc
      841 gccgatctcg tcgatctcat cgaggcgcag accatctccg gcaagatcgc caaggacgtg
      901 ctcgcgatca tcttcgccga ggagaaggac gcggcgccga gcgcgatcgt cgaagcgcgc
      961 gggcttcgcc aggtcaccga tacgggcgcg atcgaagcgg ccgtcgatcg gatcatcgcc
     1021 gccaatcccg acaaggtcgc gcaggccaag gcgaagccga ccatgctcgg ctggttcgtc
     1081 ggccagacga tgaaagcgac cggcggaaaa gccaatccgc aggccgtcaa tgacgcgctg
     1141 aagcgcaagc tcggccttta aaagagccga atcgcacgcc tcgattcggc gagtcgcgcg
     1201 cgcctcgcat cgtttgcaag cctcgagtcg catcgttcgc gacaaatttg tgtatgcggc
     1261 ggcgaagaaa aattcgattg ctcggcgcgc gcgcgatgtt ctctttcgaa acgcgaaagc
     1321 cgccgttgat gcgccctccg cgcccgcccg atgacggcga atcgtcgccg cggcgggcct
     1381 gacgccgctt cgcgcatcgt acctcgcgat tgcggaacgt ccggtaaaat aaggcctcgc
     1441 cgcaccgctt cgcgcgattc gccgccgcgc cgcgcgacgc tcgcgccggc gctgaagcgc
     1501 cgccgctcgt caaggcgttg ctttgcgcgc gcgcggcgcg cgatgcgcgt cgaaacgcga
     1561 atctgcgcag cataattcga tagagaggcg catgaagcga tgcgcgcgcg tcatgcgaga
     1621 cttgctctcg caaggaacac agctagcttt gttagcgtct gatgtctgcg cgcaacaaat
     1681 gcgcggacaa tggaatttaa gagggacgaa tcaaatggcg aaagctgctg ctaagaaatc
     1741 ctcccgcaag accgccgcca agaagacggc gcgcaagggc gccgccaaga agacggcgcg
     1801 caagggcgcc cgcaaaggcg ccgccaagaa gggcgcgcgc aagggcgcgg cgaagaaagg
     1861 cgcccgcaag ggcgccgcga aaaagaaagc ggctaagaag aaggccccag caaagcgcaa
     1921 ggctcaaaag aaaccagaac cggcgccaac cccgccgcct atggctgagc cgacatagcg
     1981 agggcgcgct tcgcgcgcat tgatgttcga gcgcggcgat cagctttcgg cttcgccgcg
     2041 catcgcgttg aggacgccgt agccttcaag gccggcgagc tccaggggcg gattttcgcg
     2101 cccatccatc agccggcgcc tctcttccgc ttgacgaagc ttcgccgaat gcgccaaatg
     2161 ccgcgcgttc gcgcccgggt ttagagccgt ggcgccatcc cgcggagacg tggccgagag
     2221 gctgaaggcg gcggtttgct aaaccgttat agggttgtaa agccctatcg agggttcgaa
     2281 tccctccgtc tccgccaatt cccgctgccc tgacgcttcg gtcgcaccct aagtgctttc
     2341 gtcctcaaac cggtttgctt cgatcctcct cgcgccgtgg cggcggctta cggcggccgg
     2401 cccgctcgcg ctgagagctg ctgaagaacc ttttttgaat gagaacgccg cttggaccgc
     2461 ctcagaacac ttcgatttgt gcgtggtggc cgggggcggc cgccagacgc cggtctcgcg
     2521 tgagcaacgg cgcgtcgagc gcttcggcaa gcgcgacata cgcggcgtcg taggctgtga
     2581 cattgttccg caaagcccaa atgcgcggca aaaggaaatg gtgcggatgt cgcgtcaatg
     2641 gaaagtcggc gagatcgaca agagcggcgc ggccgcgctc cgtctcgatc tcgcccttcg
     2701 ccgcaaggcg gcgaattatt tgcgcgactt caatatcgat caaatggggc gcgtagagcg
     2761 attgtcgaga gttgaacagc cgctcgctga ccgtttttgc cgccggcgtc tggagaagcg
     2821 tctcgagaag cgccgaagca tcaacgacga tcatcggtta tcgcgttcgg cgcgaataag
     2881 ttcggcaggc gacacagaag gattcacgct cgaccggcgc tccaggcgcg cgcgcatctc
     2941 gtcgagagtt ggccgcgccg ccacttggcg gatctcattg agcagatagt cagagagcga
     3001 catccccgct aacgcggcgc gagacttaag ctgacggtgg agcccatcgg gcacgttacg
     3061 aatctgaatc atagcagaca tgcgatgaac ataagaacat gtgcatcgca tgtcaagatt
     3121 tccggggtta aaacgtcccg ctatttcccg tagctctgca gcatgccgac gcaacgggcg
     3181 gacaatttgc tgcggttcag ttcgaggcat tgcaggtagg cgtcggcgcc gtccgattcc
     3241 ttcaccttgt ggcagaactt gcgaacgtcg ggcgcgcagg ccgcgcgctg ctcgggcgtg
     3301 cccatgtccg cataggccgc ggtcgaggcg ccgagaagga tagctcccaa taacatgctg
     3361 cgaagcatcg tttattcgtc tcctggttgg ggcggcgccg gtcgatgaag aagcggcgcg
     3421 gcccgggaaa gtcggcaacg cttaaagacg ggagaatgca gcgccaagat ggcgcccaac
     3481 ggatttgaag aattatccac gcttgcgccg cgcgccgcgc ggttgaaatc gcgggctgaa
     3541 attgtgcttg aagcggcggc gccgaagctt catccctctc ttcggagaga gcaggggagg
     3601 cgcgcatgag gggtcaagcg gagatcgccg tcaaggccgg caacgcgctc ggcgaaggga
     3661 tcgtctggtc gccggcgcat ggcgaagtcc aatggaccga cattcacggc caggcgttct
     3721 ggtcgttcga tcccgcgacc ggcgctgcgc ggtcgatcgc gctgccggag cggctcgcct
     3781 gcttcgcgcc gctcggcggc gcgcgcattc tagccggctt cgccggcggc ctggcgcgtt
     3841 tcgacctcgt ctccggcgcg cgccaggaca tcgccgcgat cgaaccggaa gaggggcgca
     3901 cgcgcctgaa cgacggcaag ctcgaccgcc agggccggct cgtcttcggc acgatggacg
     3961 agagcaagga cgcgcagccg ctcggccgcg tctggagctt cgacggcgag cgcccgcgcg
     4021 tcctgttcgg cggcgtgcgc atttcgaact cgatcgcctt ctcgccggac ggacggcgga
     4081 tgtatttcgc cgacacgccg cagcgcgtga tctggcgctt cgattacgac agcgccagcg
     4141 gcgacgtcgc caaccgccaa gtcttcgtcg aggtcgcgcc ggatcacggc tatccggacg
     4201 gctcggcggt cgacgcggat ggctgcctgt ggaacgccg
//
LOCUS       DATGJW010000130         2913 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_23942, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000130 DATGJW010000000
VERSION     DATGJW010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2913)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2913)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2913
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23942"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..318
                     /locus_tag="VKU03_03790"
     CDS             133..318
                     /locus_tag="VKU03_03790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3008 family protein"
                     /protein_id="HLJ70413.1"
                     /translation="MPAKSAAQQKAAGAALSAKRGKTPKSKLKGASKSMLSMSEKQLE
                     DFAHTKRKGKPEHAGKR"
     gene            546..1040
                     /gene="ribH"
                     /locus_tag="VKU03_03795"
     CDS             546..1040
                     /gene="ribH"
                     /locus_tag="VKU03_03795"
                     /EC_number="2.5.1.78"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009349 - riboflavin synthase complex
                     [Evidence IEA];
                     GO_function: GO:0000906 - 6,7-dimethyl-8-ribityllumazine
                     synthase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6,7-dimethyl-8-ribityllumazine synthase"
                     /protein_id="HLJ70414.1"
                     /translation="MAEPRRDPSTPLLSLNDGRLLIVEARFYEAIGAMLMDGARRAVA
                     AAGAACDVLAVPGALEIPIAAAIALDAAAKAGRPYDGLVALGCVIRGETYHFEIVAGE
                     SSRALMDLAVARALPLGNGVLTVENESQALVRADPAQGDKGGDAARAAIALAALKRKL
                     GAAR"
     gene            1037..1489
                     /gene="nusB"
                     /locus_tag="VKU03_03800"
     CDS             1037..1489
                     /gene="nusB"
                     /locus_tag="VKU03_03800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HLJ70415.1"
                     /translation="MSADQRAAARLAAVQALYQMEVADKGLNDILAEFESHWIGREVE
                     GEQYKPAELGLFRDILAGVLADQRAIDRTLDDALAEGWPLKRLEALMRAILRAGLYEL
                     KARSDVPARVAIKEYVDIAAAFFAKEEAGMINAVLDALARKVRPKEFA"
     gene            complement(1526..1867)
                     /locus_tag="VKU03_03805"
     CDS             complement(1526..1867)
                     /locus_tag="VKU03_03805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70416.1"
                     /translation="MAFGAAILLCPLAAAAAQQGEQGGWAKLPRMQLERQFAGPLQDT
                     IIQRWRDPVDGVVCYIYLPITAAHTPPTASGFVEYGANSIGSISCVEPAPHTSSHAER
                     RSPPPEPLHQQ"
     gene            complement(1900..>2913)
                     /locus_tag="VKU03_03810"
     CDS             complement(1900..>2913)
                     /locus_tag="VKU03_03810"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HLJ70417.1"
                     /translation="NVAPGALTASSTDAVNGAQLYATNQQVATNTAAIANLDSIVTGI
                     AGSSNYYKANSTGPAPSATGANALAMGESAQSSGANAIAVGSNAQATQSGSIAMGMNA
                     ASTGANAIAIGTGATATGSVAMGNSASAANGGAAFGDFSVATGGVANVSSATAIGNSA
                     SATTANAVALGQSASVAAANGTALGAGATVQASASGSVAIGQGSVASAPNTVSVGAPG
                     AERRIVNVAPGVAPTDAVNVSQLDSVAAGIQSQIGGLQSQVNRANAGVAMAMAMGGGF
                     LPDNKKFALAANYGTFAGENALAVTGLLRVTDNVVFTGSAGFGVGNEAHDVGGRVGVQ
                     VAW"
BASE COUNT          448 a         1007 c         1013 g          445 t
ORIGIN      
        1 tctcggtctc cccggtctcc gaggcggagt gggcgacgat actgaaaatg gcggagggct
       61 gacggggccg taggatcgca gaagcgccgc ctgcgttagg cggggcgggg aggccagtcc
      121 atggaggtaa ccatgcccgc caaatccgcc gctcagcaaa aggccgccgg cgcggcgctt
      181 tccgccaagc gcggcaagac gccgaagagc aagctcaaag gcgcgtcgaa atccatgctg
      241 tcgatgagcg aaaagcagct cgaggacttc gcccacacca agcgcaaggg caagcccgag
      301 cacgccggga agcgctgagc cccatcctcc cccgcgaaag cgggggaggg ggaccatgcg
      361 aagcatggtg gagggggccg cggtttctca ggacaacccc caaccgccgg cggccccctc
      421 caccgcgctg acgcgcggtc ccgctccccc gtcttcgacg ggggaggatt gtcttgcctc
      481 gcgcggccgg cccctttatg ccctcgcgcg gcgccagcgc cattgcccat tcatgaccag
      541 gacccatggc cgaaccgcgc cgcgatccct cgaccccgct tttgagccta aatgacggcc
      601 ggctgctcat cgtcgaggcc cgcttctacg aggcgatcgg cgcgatgctg atggacggcg
      661 cgcgccgcgc cgtcgcggcc gccggcgcgg cctgcgacgt gctcgccgtg cccggcgcgc
      721 tcgaaattcc gatcgccgcg gcgatcgcgc tcgacgctgc ggccaaggcc gggcgtccct
      781 atgacgggct ggtcgcgctg ggctgcgtca ttcgcggcga gacctatcat ttcgagatcg
      841 tcgccggcga atcctcgcgc gccttgatgg atctcgccgt cgcccgcgct ttgccgctcg
      901 gcaatggcgt cctcaccgtc gagaacgaga gccaggcgct ggtgcgggcc gatccggccc
      961 agggcgacaa gggcggcgat gcggcgcgcg cggcgatcgc gctcgcggcc ctgaaacgca
     1021 aattgggcgc cgcccgatga gcgccgatca gcgcgccgcc gcgcgcctcg ccgccgtgca
     1081 ggcgctctac cagatggagg tcgccgataa gggcctcaac gacattctcg ccgaattcga
     1141 atcgcattgg attggccgtg aggtcgaagg cgagcaatat aagccagccg agctcggcct
     1201 gtttcgcgac attctcgccg gcgtcctcgc cgatcagcgc gccatcgacc gcacgcttga
     1261 cgacgcgctc gccgaaggct ggccgctgaa gcgcctcgaa gccttgatgc gcgccatcct
     1321 acgcgccggc ctctacgagc tcaaagccag gtcggacgtg ccggctcgcg ttgcgatcaa
     1381 ggaatatgtc gacatcgccg ccgccttctt cgccaaggag gaggcgggga tgatcaatgc
     1441 ggtgctcgac gccctggcgc gaaaagtccg gccgaaggaa ttcgcttgaa agtacgaacc
     1501 tctctccttt cgggagaggg gagcgctact gctgatgcag cggctcgggc ggcggcgagc
     1561 gccgctcggc gtgcgaggat gtatgcggcg ccggctcgac gcaactgatc gagccgatcg
     1621 aatttgcgcc gtattcgacg aagcccgacg cggtcggcgg cgtgtgggcg gcggtgatcg
     1681 gcagatagat atagcagacc acgccgtcga ccggatcgcg ccagcgctgg atgatcgtat
     1741 cctgcagcgg gccggcgaat tggcgctcga gctgcatacg cggcagtttc gcccagccgc
     1801 cttgctcgcc ttgttgcgcc gccgcggcgg cgagcgggca gagaagaatc gccgcgccga
     1861 acgccaagag cgaccgccgc agcctcagag cccgaacgcc taccatgcga cttgcactcc
     1921 gacgcggccg cccacgtcat gggcctcatt gccgacgccg aaaccggccg agcccgtgaa
     1981 cacgacattg tcggtgacgc gcagcaggcc ggtcaccgcc agggcgttct cgccggcgaa
     2041 tgtcccgtaa ttggcggcga gcgcgaattt cttgttgtcg ggcaggaagc cgccgcccat
     2101 cgccatcgcc atggcgacgc cggcattggc gcggttgacc tgcgattgca ggccgccgat
     2161 ttgcgactgg atgcccgccg cgacgctgtc gagctgcgag acgttgaccg cgtcggtcgg
     2221 cgcgacgccc ggcgcgacat tgacgatccg ccgctcggcg cccggcgcgc cgaccgacac
     2281 cgtgttcggc gcgctcgcca ccgagccctg gccgatcgcc accgagccgc tcgccgaggc
     2341 ttgcaccgtc gcgccggcgc cgagcgccgt accgttcgcg gcggcgacgc tggcgctttg
     2401 gccgagagcg acggcgttgg cggtggtcgc gctcgccgaa ttgccgatcg cggtcgccga
     2461 cgagacgttg gcgacgccgc cggtcgccac ggaaaaatcg ccgaacgcgg cgccgccatt
     2521 ggcggccgag gccgaattgc ccatcgcgac cgagccggtc gccgtcgcgc ccgtgccgat
     2581 ggcgatggcg ttggcgccgg tcgaggcggc gttcatgccc atggcgatcg agccggattg
     2641 cgtcgcctgc gcattggagc cgacggcgat ggcgttggcg ccgctgcttt gcgcgctctc
     2701 gcccatggcg agcgcgttgg cgccggtcgc tgatggggcc gggcctgtgc tgttcgcctt
     2761 gtaataattc gagcttccgg caatgccggt gacgatgctg tcgagattgg cgatcgccgc
     2821 cgtattcgtc gccacctgct ggttggtggc gtaaagctgt gcgccattga ccgcgtcggt
     2881 cgaactcgcg gtgagcgcgc cgggcgcgac att
//
LOCUS       DATGJW010000131         6229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_128808, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000131 DATGJW010000000
VERSION     DATGJW010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6229
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128808"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /gene="uraD"
                     /locus_tag="VKU03_03815"
     CDS             <1..634
                     /gene="uraD"
                     /locus_tag="VKU03_03815"
                     /EC_number="4.1.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008935343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0019628 - urate catabolic process [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="2-oxo-4-hydroxy-4-carboxy-5-ureidoimidazoline
                     decarboxylase"
                     /protein_id="HLJ70418.1"
                     /translation="GRPGRAASLARFLDYVRGHDKVWVARRIDIARHWISRHPPAGGW
                     RPSHMTRPLFIERFGGVYEHSPWIAAAAYDAGLGPQADKAEGLAAALSAAMRKGTDAQ
                     KRALVEAHPDLAGKLAQAKRLTPESAGEQASAGLDHLTDAERARFTSLNDTYRARFGF
                     PFIFAVKGRNKAEILASFEQRLKNDADTEFATALDQIDRIAALRIKDILS"
     gene            631..1947
                     /gene="guaD"
                     /locus_tag="VKU03_03820"
     CDS             631..1947
                     /gene="guaD"
                     /locus_tag="VKU03_03820"
                     /EC_number="3.5.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008892 - guanine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0006147 - guanine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanine deaminase"
                     /protein_id="HLJ70419.1"
                     /translation="MSGAARVVRGRILSFSDEPRIAGASALNLIEKGAVLVEDGLIKA
                     VGEASDVVRKAPGGVKIDDHGDKLILPGLIDTHIHYPQTRVIASYGAQLLEWLQKYTF
                     IEEQKLRDPAHAAIVARFFLDELFRQGTTTAMVYCTTHPESVDAFFAESEKRGARMIA
                     GKVMMDRNAPEGLRDTPQRGYDETKALIQRWRGKGRLDYAITPRFAPTSTDAQLEASG
                     ALARENPEAYVQTHLCENHAEIAFVKELFPWTKTYTEVYQRYGLLGPRTVLGHCIHLE
                     DSEVAMLAESGAVAAFCPTSNLFLGSGLFDMRRLQEHAPPVRVALATDVGGGTSYSML
                     QTANEAYKVLQMNGQTWPAAQAFYQMTLGNARALGLGDRIGAVSEGFEADLVVLDSRA
                     TPAMAHRMEAVTGDLAEELFILMTMGDDRTVSETYLAGRPVKSAAS"
     gene            2205..3404
                     /locus_tag="VKU03_03825"
     CDS             2205..3404
                     /locus_tag="VKU03_03825"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HLJ70420.1"
                     /translation="MLKSKLDRLIKVGRLSIAWPNGEVWRFGDQGSAGPDVAVRLESS
                     FAAAKIAVHPHLYLGEAYMDGELTMERGGVFDLLDLLGRNLALREAPPNLLQRWARRP
                     LALLQQYNSQRSARRNVARHYDFSERLYRAFLDVDLQYSCAYFARPDMTLEEAQTAKR
                     RHIAGKLLLKSGQRVLDIGCGFGGLALHIARSADVEVLGVTLSREQLDVAKRRAREAG
                     LDGRVRFELADYRALDGRFDRIVSVGMFEHVGKPQYPKFFAVIARLLDPKGVALLHAI
                     GRMRGPGLTSAWIRRYIFPGGYIPALSDVAPIIERSGLWLTDLEVLRLHYAETLRHWR
                     QRFLANRSSLADLYDDRFCRMWEFYLAASETSFRHGGFMVLQAQLSHAPDAAPLTRDY
                     MTDADRS"
     gene            3529..4821
                     /locus_tag="VKU03_03830"
     CDS             3529..4821
                     /locus_tag="VKU03_03830"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HLJ70421.1"
                     /translation="MTSLRSVAQDSETIGAAMRAVGAAARAAARALANAPRAQKDRAL
                     TQAAAALRDRAAEILAANSRDLAAAKARGVAAAFLDRLTLDEKRIETMARGLEEIAAL
                     PDPVGRVLARFERPNGLLIERVATPLGVVGVIYESRPNVTADAGALCLKSGNAAVLRG
                     GSDSFHSSAAIHACLVEGLKGAGLPEAAISAAPSASREAVAEMLAGLGGSLDVIVPRG
                     GRSLVERVQKEARVPVFAHLEGVVHVYVDRAADLDKAKRVLINSKLRRTGVCGAAEKL
                     LVDKACAATHLKPLVEALLERGCAVRGDAAAMAADPRVTRASEEDWRAEYLDAIIAVK
                     VVDGLDGAIAQIETYGSHHTDCILTEDKDAADRFLKEVDSAIVLHNASTQFADGGEFG
                     FGGEIGIATGRMHARGPVGLEQLCTFKYRVHGDGQLRP"
     gene            complement(4994..5479)
                     /locus_tag="VKU03_03835"
     CDS             complement(4994..5479)
                     /locus_tag="VKU03_03835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLJ70422.1"
                     /translation="MPDSALDRFDLALLDLVQRDNLTPARALAERVGLSESAVLRRLR
                     RLRREKIIVADVSIVQAAAIGLPLTIIVLASLERESAKVLDAFARRVQARPEVRQCWY
                     VTGDADWVLVLQVESMASYEAFTREMFLADPNVKGFKTLVAMREVTGGSPLSRSEMGR
                     G"
     gene            5566..>6229
                     /locus_tag="VKU03_03840"
     CDS             5566..>6229
                     /locus_tag="VKU03_03840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011930799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HLJ70423.1"
                     /translation="MPDSARTIDDESPAALLRLCPAHAPTPLVAAPRLAAALGLAGVL
                     VKDEGRRALGSFKALGGVYAGLRALARAARLPTASLLERGRAARTFPALVCASDGNHG
                     LAVAAAARLAGTPALVFLPDIVPAARAARIAAKGAEIVRVAGTYDDAVRAAARAARER
                     GGLLIADTSGDEGDPVVAAVMAGYQIVADEIRAQLFGGAHATPTHLFVQAGVGGLAAA
                     LAA"
BASE COUNT          998 a         2107 c         2074 g         1050 t
ORIGIN      
        1 cggccggccg ggccgcgccg cttcgctggc gcggttcctc gactatgtgc gcggccacga
       61 caaggtctgg gtggcgcggc gcatcgacat cgcccggcat tggatttccc gccatccgcc
      121 ggccggcggc tggcggccgt cgcatatgac gcgcccgctc ttcattgagc gcttcggcgg
      181 cgtctacgaa cattcgccgt ggatcgccgc cgcggcttac gacgccggcc tcgggccgca
      241 agcggacaag gccgagggcc tcgccgccgc tttatcggcc gcgatgcgca aagggacgga
      301 cgcgcagaag cgcgcgctgg tcgaagcgca tcccgatctt gccggcaaat tggcgcaggc
      361 gaaaaggctc acgccggaat cggccggcga gcaggcgagc gccgggctcg accatctgac
      421 ggacgccgag cgcgcgcgct ttacctcgct caacgacacc tatcgcgccc gattcggctt
      481 tccgttcatc tttgcggtga aaggccgcaa caaggccgag attctcgcca gcttcgaaca
      541 gcgcttgaag aacgacgcgg atacggaatt tgcgaccgcg ctcgatcaaa tcgaccgcat
      601 cgccgccttg cggatcaagg atattctctc gtgagcggag ccgcccgcgt cgtccgcgga
      661 cgaatccttt ccttcagcga tgagccgcgg atcgccggcg cgagtgcgct gaacctgatc
      721 gagaaaggag ccgtccttgt cgaggacggc ctgatcaagg cggtcggcga agcgagcgac
      781 gtcgttcgca aagcgccggg cggcgtcaag atcgacgatc atggcgacaa gctgatcctg
      841 ccgggcctca tcgacacgca tattcactat ccgcagaccc gcgtcatcgc gtcctatggc
      901 gcgcagcttc tcgaatggct gcagaaatat acgttcatcg aggagcagaa gctgcgtgat
      961 cccgcccatg cggcgatcgt cgcgcgtttt tttctcgacg agcttttccg ccagggaacg
     1021 accacggcga tggtctattg cacgacccat cctgaatccg tcgacgcctt tttcgccgaa
     1081 agcgagaagc gcggcgcgcg gatgatcgcc ggcaaggtga tgatggaccg caatgcgccc
     1141 gagggcttac gcgacacgcc gcagcgcggc tatgacgaga ccaaggcgct gatccagcgc
     1201 tggcgcggca agggccggct cgactatgcg atcacgccgc gtttcgcgcc gacctccacc
     1261 gacgcgcaac tcgaagcgtc gggcgcgctg gcgcgcgaaa accctgaagc ctatgtccag
     1321 acccatcttt gcgagaacca cgccgagatc gccttcgtca aggaactgtt cccctggacg
     1381 aagacctaca cggaagtgta ccagcgctat ggcctgctcg ggccgcgcac ggtgctcggc
     1441 cattgtatcc atcttgagga cagcgaagtg gcgatgctgg ctgagagcgg cgccgtcgcg
     1501 gcgttctgcc cgacttccaa cctgtttctc ggctccgggc ttttcgatat gcgtcgccta
     1561 caagagcatg cgccgcccgt ccgcgtcgcg ctggcgaccg atgtcggcgg cggcaccagc
     1621 tattcgatgc tgcagacggc caacgaagcc tacaaggtcc tgcagatgaa cggccagacc
     1681 tggccggcgg cgcaggcttt ctaccagatg acgctcggca acgcccgagc gctcggcctc
     1741 ggcgaccgga tcggcgctgt gagcgaaggg ttcgaggccg atctggtcgt gctcgattcc
     1801 cgcgccacgc ccgccatggc ccatcgcatg gaggcggtga cgggcgatct cgccgaggaa
     1861 ttgttcattc tgatgacgat gggcgacgac cgcacggtca gcgagaccta tctcgcgggc
     1921 cggccggtga agagcgccgc ttcctgacgc gcctcgtatt tccggacggg cttggaggcg
     1981 cggtcgttca ccgcgccgcc tccgccgtcg cttctccgcg aaacggggcg cttcgcgccc
     2041 gccgcggtca gcgaccgagg ctacaacggg ccgagcatcc ggaaatttgc ccgattgcgt
     2101 caggcgccag cgcaacccgg gtcatggcgc ggcgcgtggt aggatagcgg ggtcgatatc
     2161 gtcggcttgc gcccattctc gtttccccga acaattgtgc gcttatgctg aaatcgaagc
     2221 tcgatcgtct catcaaggtc ggacggctgt cgatcgcctg gccgaacggc gaggtttggc
     2281 gtttcggcga ccaaggctcg gccggcccgg acgtcgccgt gcgcctggaa tcgtcgttcg
     2341 ccgcggcgaa aatcgccgtc catccgcatc tctatctcgg cgaagcctat atggacggcg
     2401 agctgaccat ggagagggga ggcgtcttcg acctgctcga tctgctcggc cgcaacctcg
     2461 cgctccgcga agcgccgccc aatctgctcc agcgctgggc gcggcggcct ttggcgctgc
     2521 tccagcaata caattcgcag cgctcggccc ggcgcaacgt cgcgcgccat tacgatttct
     2581 ccgagcgcct ctatcgcgcc tttctcgacg tcgacctgca atattcctgc gcctatttcg
     2641 cccggccgga catgacgctc gaagaggcgc agaccgccaa aaggcggcat atcgccggca
     2701 agctgctgct caaatccggt cagcgcgtgc tcgacatcgg ctgcggcttc ggcggcctcg
     2761 ccttgcatat cgcgcgcagc gccgatgtcg aggtgctcgg cgtcaccttg tcgcgcgagc
     2821 agctcgatgt cgcgaaaagg cgggcgcgcg aagccgggct tgacggccgc gtccgcttcg
     2881 agcttgccga ttaccgcgcc ctcgacggcc ggttcgaccg catcgtctcg gtcggcatgt
     2941 tcgaacatgt cggcaagccg caatatccga aattcttcgc cgtgatcgcc aggcttctcg
     3001 atccaaaggg cgtagcgctg cttcatgcga tcggccgcat gcgcgggccg ggcctgacca
     3061 gcgcctggat ccggcgctac atttttcccg gcggctatat tccggctttg tcggacgttg
     3121 cgccgatcat cgagcgcagc gggctgtggc tgaccgatct cgaagtgctg cgcctgcact
     3181 atgccgagac gctgcgccat tggcgccaac gcttcctcgc caaccgctca tcgctcgccg
     3241 atctctatga cgaccgcttc tgccggatgt gggaattcta tctggcggcg agcgagacga
     3301 gttttcgcca cggcggcttc atggtgctcc aggcgcaatt gtcgcacgcg cctgacgccg
     3361 cgccattgac ccgcgactac atgaccgacg cggatcggag ttaacacgcg cgcccccctc
     3421 cccgccttgc gcgccttgcg gggagaaggg tttctcctgc cgatttgagc ccaccagacc
     3481 ggacatgcta aaaggccgaa caaatttcaa cctcgctcag ggacggcaat gacctcgctc
     3541 cggtctgtcg cgcaggattc ggagactatc ggcgcggcga tgcgcgccgt cggcgccgct
     3601 gcgcgcgcgg cggcgcgcgc gctcgccaat gcgccgcgcg cgcagaagga ccgcgccttg
     3661 acgcaagccg ccgccgcgct gcgcgaccgc gccgcggaaa ttctcgccgc caattcccgc
     3721 gatctcgccg ccgccaaggc gcgcggcgtc gcggcggcct ttctcgatcg actgacgctg
     3781 gacgagaagc gcatcgagac gatggcgcgc gggctcgaag agatcgccgc cttgcccgat
     3841 ccggtcggcc gcgtcctggc gcgcttcgag cggccgaacg ggcttctcat cgagcgcgtg
     3901 gcgacgccgc tcggcgtggt cggcgtcatc tatgagagcc ggccgaacgt caccgccgac
     3961 gccggcgcgc tgtgcctgaa atcgggcaac gccgccgtgc tgcgcggcgg ctcggacagt
     4021 tttcattcgt cggcggcgat ccatgcctgc ctggtcgaag gactgaaagg cgcgggcctg
     4081 cccgaggcgg cgatttcagc cgcgccgtcc gcctcgcgcg aagcggtcgc cgaaatgctg
     4141 gccggccttg gcgggtcgct cgatgtgatc gtgccgcgcg gcggccgcag cctggtcgag
     4201 cgcgtgcaga aggaggcgcg cgtgccggtc ttcgcccatc tcgaaggcgt cgttcacgtc
     4261 tatgtcgacc gcgccgccga tctcgacaag gcgaaaaggg tgctgatcaa ctccaagctg
     4321 cggcgtaccg gcgtttgcgg cgcggccgag aagctgctgg tcgacaaggc ttgcgcggcg
     4381 acgcatctga agcccttggt cgaggcgctg ctcgaaaggg gctgcgccgt gcgcggcgac
     4441 gccgccgcca tggccgccga tccgcgcgtg accagggcga gcgaggagga ttggcgcgct
     4501 gaatatctgg acgcgatcat cgccgtcaaa gtggtcgacg ggctggacgg ggcgatcgcg
     4561 cagatcgaga cctatggctc gcatcatacc gattgcatcc tcacggagga caaggacgcc
     4621 gccgatcgtt tcctcaagga ggtcgattcc gccatcgtcc ttcacaacgc ctcgacccaa
     4681 ttcgctgatg gcggcgagtt cggcttcggc ggcgagatcg gcatcgccac cggccgcatg
     4741 catgcgcgcg gcccggtcgg gctggagcaa ctctgcacct tcaagtacag agtgcatggc
     4801 gacgggcagt tgaggccgta agcgaggcgc gtcccctccc ccgaagggag aagggcaggg
     4861 gtgcagacga gtcggaaaga ggcgcttcgg taggttttat agtacgtcgt cgaacttgat
     4921 gtccgaaaat agtcctgagc gtttcgcctg ttctgtaaat tcgttttggt gcgaaattcc
     4981 gacagccgta ccctcaccct cggcccatct cgcttcggga gaggggtgat ccgcctgtca
     5041 cttcccgcat cgccaccagc gtcttgaagc ctttcacatt gggatcggcg aggaacattt
     5101 cgcgcgtgaa ggcttcgtag ctcgccatcg actcgacctg gagaaccagc acccagtcgg
     5161 cgtcgccggt cacgtaccag cattggcgca cctccggccg cgcttggacg cggcgggcga
     5221 aggcgtcgag aaccttggcg ctctcgcgct cgagcgaggc gaggacgatg atcgtcagcg
     5281 gcaggccgat ggccgccgcc tggacgatcg aaacgtccgc gacgatgatt ttctcgcgcc
     5341 gcaagcgtcg gaggcgccgc agcacggcgc tttcggaaag gccgacgcgc tcggccaaag
     5401 cgcgcgccgg cgtcagattg tcgcgctgga cgaggtcgag cagcgcaaga tcgaatcgat
     5461 cgagagcgga atcaggcatg ggaacccgga tattccgcca gaaacaggcg ctcagacaac
     5521 caaattcgcc gccaatcggc gcccgcggcg cgttagattg ccgccatgcc cgattccgcg
     5581 cgaaccatcg atgacgagag tcccgcggct ctgctccggc tttgcccggc ccatgctccg
     5641 acgccgcttg tcgcggcgcc gcgccttgcg gcggcgctcg gccttgccgg cgtgctggtc
     5701 aaggacgagg ggcgccgcgc gctcggcagc ttcaaggcgc tgggcggcgt ctatgcgggc
     5761 ttgcgggcgc tcgcccgcgc ggcccgtctt ccgaccgcgt ccttgctgga gagggggcgc
     5821 gcggcgcgga cgtttccggc gctggtctgc gccagcgacg gcaaccacgg cctcgccgtg
     5881 gcggcggcgg cgcggctggc ggggacgccg gcgctggtct tcctccccga catcgtgccg
     5941 gcggcgcggg ccgcccgcat cgccgccaag ggcgccgaaa tcgtccgtgt cgcagggacc
     6001 tatgatgacg cggtgcgcgc cgcggcccgg gcggcgcgcg agcgcggcgg cctgctgatc
     6061 gccgatacga gcggggacga aggcgatccc gtcgtcgccg ccgtcatggc gggttatcaa
     6121 atcgtcgccg acgaaatccg cgcgcagctc tttggcggcg cgcacgccac gccgacccat
     6181 ctcttcgtgc aggcgggcgt cggcgggctt gccgcggctt tggcggccg
//
LOCUS       DATGJW010000132        20223 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_438523, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000132 DATGJW010000000
VERSION     DATGJW010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 20223)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20223)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20223
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438523"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1466)
                     /gene="der"
                     /locus_tag="VKU03_03845"
     CDS             complement(72..1466)
                     /gene="der"
                     /locus_tag="VKU03_03845"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HLJ70424.1"
                     /translation="MSAKVAIIGRPNVGKSTLFNRLVGRKLALVDDAPGVTRDRREGE
                     ARLGDLSFTVIDTAGLEEGEAATMAGRMRAQTEAALADCDAILFLIDARAGLTPTDRH
                     FAQLIRRAGKPILLVANKAEGKAGEAGAYEAFSLGLGEPLPLSAEHGEGLGDLYDALA
                     AVLPSEAREPESGAEAPTLELGDEEDGSEVDLSKPLRIAVLGRPNAGKSTLLNRILGE
                     DRLLTGPEPGLTRDSIGVETVWRGRRLKLFDTAGLRRRARVVEKLEKLAAADALRAVR
                     FAEVVVLLLDATIPFERQDLTLADLVEREGRALVIGLNKWDLVQDKSQAAKTLREEAD
                     RLLPQLRGARVVPVSGLTGAHVDRLMEAVIATEQIWSKRIATGRLNRWLGPVVDATPP
                     PAVAGRRIKIRYMTQPKARPPHFVLFGNQLQALPDSYKRFLVNGLRETFDLPGAPIRL
                     SLRTSTNPYEKKRR"
     gene            complement(1505..2164)
                     /locus_tag="VKU03_03850"
     CDS             complement(1505..2164)
                     /locus_tag="VKU03_03850"
                     /inference="COORDINATES: protein motif:HMM:NF021492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ70425.1"
                     /translation="MSDIFREVDEEVRRDQAVEFWKKYQNYIIAAVILLLLATAGWRF
                     YEWRRIEAAQAAGARFEDALSLERSGKSGEASADFAKLAADAPKGYAILARMADAASL
                     AKSDPNRAIAAYDKLSDDESIGPLFREAARLRAAALRLDNGQAEQAKKALEGLAAPTG
                     AFRSTARELLGAASLAAGDFDGAGKWLDMIAADPESPPAARQNAEMMLGLVRSGKPAA
                     K"
     gene            2377..2634
                     /locus_tag="VKU03_03855"
     CDS             2377..2634
                     /locus_tag="VKU03_03855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70426.1"
                     /translation="MKTLRLGLLVGSLAFGLAACTTTQEQLGGAGTGVVAGAAVAGPV
                     GAVVGGVAGAITGPSVAHAAGAPHHHRHYYWRHGHRYYTYY"
     gene            2692..3213
                     /locus_tag="VKU03_03860"
     CDS             2692..3213
                     /locus_tag="VKU03_03860"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLJ70427.1"
                     /translation="MLRLLLLRHAKAVPHDPARDHGRALAERGRADAKRLGKLLAGES
                     RAIEAAVHSGARRAEETALIALGELPKGVPVSAEPRLYEATPAGVIAALRDLPDAATT
                     ILVVGHNPSLAEAALRLAGSGDQAALARMAAKYPTCGLATIDFDVADWKEISAGGGRL
                     VAFLAPAGLGGSD"
     gene            3282..4724
                     /locus_tag="VKU03_03865"
     CDS             3282..4724
                     /locus_tag="VKU03_03865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YcjX family protein"
                     /protein_id="HLJ70428.1"
                     /translation="MSLFHNFIDEAGAAARSFADYLAGGALRLGVTGLSRAGKTVFIT
                     ALINNLVAGVRLPVLSASAEGRIARARLEPQPDDAVPRFAYEDHLAALSGQERRWPES
                     TRRISELRVTIEFERAHGWGRRASTLTLDIVDYPGEWLLDLPLLDKSYAQWSRETFEA
                     SNMPARRPLAADWLAFAGALDPSAAAEEDEARKSAALFTAYLRKAREATYALSTLPPG
                     RFLMPGDLEGSPALTFAPLRVETGEAYSPHSLAAMMERRYESYKSHVARPFFRDHFAR
                     LDRQIVLIDALSALNSGPAAVRDLEHALFDVLRAFRAGRASLLSTIFRPRIDRILFAA
                     TKADHLHHASHDRLENILRFLTAKAIARAEGLGAAIDVIALAAIRATREAKIRQSGET
                     LDAIIGTPERGERLGGEIFDGVSEAAIFPGQLPDNPAAVFDGDAIGRSEAESDWRFLR
                     FRPPKVAFGEAPPHIRLDRALQFLIGDRLA"
     gene            4721..5812
                     /locus_tag="VKU03_03870"
     CDS             4721..5812
                     /locus_tag="VKU03_03870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01620 family protein"
                     /protein_id="HLJ70429.1"
                     /translation="MNESRKKSDEAAETRPRPRAFRLDDARVAVADSETTPRHAGIVI
                     EPQAAPIPDRAREPPIEEGEREIEEAQRAGMAPAWRPKLATLFWSGLGGLVSLGIGLW
                     ANQLIETLFQKAQALGWIGLALGAMTLFAAFALVLREVAAILRQSAIAKLHAALALAR
                     ANDDRDAARKLTMRLCALYEKRPETARGRAEIADAAVQIIDGRDLIDIAERALLRPLD
                     AKAQSEIATAAKRVSMVTAISPRAILDMLFVVAQIIRLIRRIAEIYGGRPGLFGFFKL
                     ARSVAAHVAITGGMAVGDSLLQQLVGHGIASKLSARLGEGVLNGLLTARVGLSALAVC
                     RPAPFGVEKPPGVADVAPFLLTGGKERRT"
     gene            5935..6543
                     /locus_tag="VKU03_03875"
     CDS             5935..6543
                     /locus_tag="VKU03_03875"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HLJ70430.1"
                     /translation="MKNGGLRFGPLGRHCVHLCVDMQRMFAEETVWRTPWLRRVLPNI
                     LRLVESRPAATVFTRFIPADRPGEGPGAWRRYYERWSSMTLQNLSRDMVELVPELAMF
                     VPPAAIFDKRVYSPWRGGALHGDLLRRSIDTLLVSGGETDVCVLSTVLGAIDYGYRVV
                     IVTDALCSSSDETHDALLALYETRYGQQIEAVTADEAIESWK"
     gene            complement(6646..6825)
                     /locus_tag="VKU03_03880"
     CDS             complement(6646..6825)
                     /locus_tag="VKU03_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70431.1"
                     /translation="MSHKTPPAPPANQSPKGPGDDKRAPAEQGRRESRAGAEKRGQEA
                     NIKQNTTNQGLQQDR"
     gene            complement(6851..7711)
                     /locus_tag="VKU03_03885"
     CDS             complement(6851..7711)
                     /locus_tag="VKU03_03885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ70432.1"
                     /translation="MNKPFPKPPFSEQQQRVPGHSAKMNPQPDYGEDTYRGAGKLKDK
                     KTLITGADSGIGRAVALAFAREGADVAVSYLNEEEDAAETKRLVEEAGRKCLLIPGDV
                     SSPDHCRKLVEKALGAFGRIDVLVNNAAHQMSFKSIGDISDEEWDKTFATNISAMFYI
                     AKAAVPHMKEGASIINTASVNADAPSPHLLAYATTKGAIQNFTGGLAQMLAEKGIRVN
                     AVAPGPVWTPLIPSTMPKEKVAHFGEQVPMKRPAQPCEVAPAYVLLASDEASYISGAT
                     IAVTGGKPII"
     gene            7864..8292
                     /locus_tag="VKU03_03890"
     CDS             7864..8292
                     /locus_tag="VKU03_03890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70433.1"
                     /translation="MLRASGRRGEITLAAEDGRIVSVLNGGLFAFTVGERAYQIARKG
                     LFSPTYELRREDSLLASAAQAAFVNRYAVECAGKAWTLKAAGLAARKFALLDGKIAVG
                     AIAPTSLNPYRETIVDLPEAIPVEAQIFLTWIVARHWDQD"
     gene            complement(8328..9698)
                     /locus_tag="VKU03_03895"
     CDS             complement(8328..9698)
                     /locus_tag="VKU03_03895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PleD family two-component system response
                     regulator"
                     /protein_id="HLJ70434.1"
                     /translation="MTARILVVDDIAPNVKLLEAKLTAEYFDVATASSGREALQKCEQ
                     DLCDIVLLDVMMPGMDGFEVCRRIKTNPATAHLAVVLITALDQPADRLRGLEAGADDF
                     LTKPIDEVALMARVRSLVRLKVVVDELRNRANTTLALRVEDENAALLAEDDRPGRILL
                     VDDRESSRERICQTLGARHEVVAEADAQEALFKAAEDNYDLFVVSLGLANYDGLRFCS
                     QIRALERTRHLPILVIAEMEDRDRVLRGLELGVNDYLSRPIDRNELLARARILLRGKR
                     YADRLREKVQHSIELSIVDPLTGLNNRRFLENHLAATLEQARARHKPVSLMILDIDHF
                     KQVNDTYGHQAGDEVLKGFADRIRRIIRGGDLLCRMGGEEFVVVMPNVPLDVAGKVAE
                     RARASIEEAPFVIDKTGLTIPITVSIGLAERGRDAEADALYERADRALYRSKAEGRNR
                     VTADAA"
     gene            complement(9703..10074)
                     /locus_tag="VKU03_03900"
     CDS             complement(9703..10074)
                     /locus_tag="VKU03_03900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ70435.1"
                     /translation="MSKTVLIVEDNELNMKLFNDLLETRGCRVLQTRQGVEAMDLARK
                     HRPDLILMDIQLPEVSGLQVTQWLKDDDDLRAIPVIAVTAFAMKGDEEKIRQGGCEAY
                     LSKPISVAKFFETVDSFLGPR"
     gene            10177..10485
                     /locus_tag="VKU03_03905"
     CDS             10177..10485
                     /locus_tag="VKU03_03905"
                     /inference="COORDINATES: protein motif:HMM:NF023518.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3572 domain-containing protein"
                     /protein_id="HLJ70436.1"
                     /translation="MYTRPKSRSSTDEEEAQGLAIDALAWLAEDLERLQRFLAVSGLG
                     PQNLRSAASEPGFLGAVLDYLAANEHLLIAFATHRNVTPESVMRGRERLSGGRAQDEE
                     "
     gene            10482..11792
                     /locus_tag="VKU03_03910"
     CDS             10482..11792
                     /locus_tag="VKU03_03910"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HLJ70437.1"
                     /translation="MSAGPWICRDCVSSFSSAAAAVRRCSQCGSPRIVADPVHNELTI
                     AHVDCDAFYAAIEKRDNPALRDRPVIVGGGGRRGVVSTCCYIARISGVRSAMPMYQAR
                     RLCPEATVIPPDMGKYAEVGRQIRAMMQRLTPLVEPLSIDEAFLDLSGCERLHGASAA
                     ATLARFAKDVETKVGVTVSIGLSYCKFLAKFASDLDKPRGFSIVARDQALALLAPQPI
                     GRIWGVGRVAEARLVKAGLSTIGDVQRRSEIEMIRECGPDGARLWRLAHGIDERRVSP
                     ERETKSISAETTFDVDIADKAELERVLLRLCERVALRLKREGLAARSVTLKLRLPDFK
                     LRTRARSGAAPTQLASRLFAVARDLLAREADGAAFRLIGVAAADFAPAEEADRGDLID
                     KDVGKEKAREQAIDALREKFGASAVVRAPLLPVKRGEGVAEGDG"
     gene            complement(11872..12222)
                     /locus_tag="VKU03_03915"
     CDS             complement(11872..12222)
                     /locus_tag="VKU03_03915"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0009432 - SOS response [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLJ70438.1"
                     /translation="MCDRRADRGNLAQIRALRIRYTLPALADLESIFDYVAAHSPQGA
                     RRIQSRIKSAIALLPLHPHMGAPTEDPTIRRLVASPYPYVIFYEPVEAEIVIHAIRHA
                     ARDPKGMPGSSSSL"
     gene            complement(12171..12401)
                     /locus_tag="VKU03_03920"
     CDS             complement(12171..12401)
                     /locus_tag="VKU03_03920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70439.1"
                     /translation="MTKLLERALEAAKRLPESDQDDIARVVLQLAGADDLDPILLTSD
                     ERAAIAASKAAAARGEFATDEQIAAIWRKYGL"
     gene            complement(12546..13901)
                     /gene="serS"
                     /locus_tag="VKU03_03925"
     CDS             complement(12546..13901)
                     /gene="serS"
                     /locus_tag="VKU03_03925"
                     /EC_number="6.1.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004828 - serine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006434 - seryl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine--tRNA ligase"
                     /protein_id="HLJ70440.1"
                     /translation="MHDIKLIRDDPEAFDLGLARRGLPPQAGALIALDDKRRAAILEL
                     QKAQERRNAVSKEIGQAMGKKDFALAEALKKEVADLKTRMPELEAAEKAALSALENEL
                     AAIPNIPAADAPDGKDETENVEIRRLGEAPRFADGFAPREHFDIGETLGLMDFESAAK
                     MSGARFVVLKGALARLERALAQFMLDMHTTEHGYEEVAPPLLVRDQTMFGTAQLPKFV
                     DDQFSAMCKPDYDEAWDRANTSLEAFQSVNKYWLIPTAEVPLTNLVRESIVDEAKLPL
                     RYTAGTYCFRAEAGAAGKDTRGMIRQHQFYKVELVSITTPEQSRAEHERMTACAEEVL
                     KRLGLHYRTVALCTGDMGFASRKTYDIEVWLPGQGRFREISSCSVCGDFQARRMNARC
                     KAADAKQTRFVHTLNGSGVAVGRALVAVLENYQNSDGSVAVPDVLRPYMGGLARIEPR
                     R"
     gene            complement(13907..14785)
                     /locus_tag="VKU03_03930"
     CDS             complement(13907..14785)
                     /locus_tag="VKU03_03930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLJ70441.1"
                     /translation="MTLRDALSAIIAAVFFGLAFIAIKIGLRETTPFLLTALRFAFAA
                     FPAVLFIKPPKASFRLTTLYGLTIGVGQFGVLFVAIGLGMPVGLASLVIQLQVFITIF
                     LAWAWLGERPRPAQLVGAGVALAGIAAIGSERLHGASLGPFLLVLLAAAFWGIGNLIG
                     KIAGKIDMLAFVVWAALAVPAPLVLLSLAFDGPGRLAALAHPSLTLVLCVALLAYGAT
                     VISYGLWARLLALYSAAEAAPFALLIPIVGMVSGAVVFGEPLSAIELTGAALVMAGLT
                     FNVFGDRILGRRAKAA"
     gene            complement(14948..15724)
                     /gene="tatC"
                     /locus_tag="VKU03_03935"
     CDS             complement(14948..15724)
                     /gene="tatC"
                     /locus_tag="VKU03_03935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HLJ70442.1"
                     /translation="MTEADIEATKAPLMDHLIELRARLIRSLLAFAVAFIICFYFSRT
                     IYNILTLPYVHIVGAENAKLIATHFLEQFFTNMKLSMFGAAVIAFPIIAVQIYKFVAP
                     GLYRSERQAFLPYLIATPIFFVLGALLVYFAVMPLLIRFSVSLQQVNRPGEATIELLP
                     KVNEYLSLIMTLIFAFGVAFQLPVILTLLGQVGIIDSAYLRSKRRYAIVIVTGLAAIL
                     TPPDVISMCSLAVPMLLLYEGAIFAVQMVEKKRLAAQSSK"
     gene            complement(15721..16296)
                     /gene="tatB"
                     /locus_tag="VKU03_03940"
     CDS             complement(15721..16296)
                     /gene="tatB"
                     /locus_tag="VKU03_03940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sec-independent protein translocase protein
                     TatB"
                     /protein_id="HLJ70443.1"
                     /translation="MFDIFDPSKIFIVGVVALIVIGPKELPRVMRTVGQVVGRMRRMA
                     NEFQAQFNEAMREAELEDVKKELAAMNEAAKVEVTGVDVSFDPATLTQHDQPAAIEGA
                     KPAAEEKPVEPAAGEARSEADLDDVKKALEAMNEAAKTEAAKPAVAEAPFEAKPNGAT
                     AHSAPQTPPVETAPPLQAEFAESALREGRAP"
     gene            complement(16381..16617)
                     /gene="tatA"
                     /locus_tag="VKU03_03945"
     CDS             complement(16381..16617)
                     /gene="tatA"
                     /locus_tag="VKU03_03945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009760006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HLJ70444.1"
                     /translation="MGFSMQHLLLFAVIALLLFGGRGKISEIMGDFAKGIKSFKKGLN
                     DDEAAAPPPAQPAGAIESRQSQPVETTTDQRKVG"
     gene            16831..18858
                     /gene="shc"
                     /locus_tag="VKU03_03950"
     CDS             16831..18858
                     /gene="shc"
                     /locus_tag="VKU03_03950"
                     /EC_number="5.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051007 - squalene-hopene cyclase activity
                     [Evidence IEA];
                     GO_process: GO:0019746 - hopanoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene--hopene cyclase"
                     /protein_id="HLJ70445.1"
                     /translation="MNDRVESALNAALDWLLRNQKQGGYWVGMVESNSCMEAEWLLAS
                     HILDVELPMRDGFIRTLFHRQRPDGAWDVFPGAPKGDINSTVEVYAALIAMGESPDRP
                     ELAKARAFILSQGGLKTVRVFTRYWLALLGVWPWTDTPNLPPEFIRFPLWFPFNIYNF
                     AQWARATMVPLAVLSARRPVFPLPGGARLDELFPEGREKFDFSAPVKKAEPWSLEAFF
                     LRVDRALHWLQTRRLIPGRENAIKLCLEWMIRHQDADGAWGGIQPPWIYGLMALRNEG
                     YALSHPCIVKGLGALSAHWTYERHGGIAVQASESPVWDTLFCLLAMAETGWSPAERPA
                     MRDALVYILDNQILEPGDWSLTVKGVPPGGWAFERANLRYPDVDDTALALLVLAKLRG
                     KAGDLDGRVEAAIERGLAWTFGMQSRNGGWAAFDRDNDKAIISTIPFCNFGEALDPPS
                     VDVTAHVIEALAELGMSKKRPEIARALAFIEKEQEPEGSWFGRWGCNHIYGVGAVLPA
                     LKAIGCDMSAAHIRKAADWLVDKQNADGGWGESCESYMDASKIGRGPSTPTQTAWAIM
                     ALQAAGGHEPAIARGLDHLIATQDAGGTWDEAFYTATGFPGYGVGARTELRDPKLAEK
                     LGQGAELSRGFMISFNLYRHYFPAIALARRLRSEAQALDAHSSALRAPVGT"
     gene            complement(18940..19341)
                     /locus_tag="VKU03_03955"
     CDS             complement(18940..19341)
                     /locus_tag="VKU03_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70446.1"
                     /translation="MPYLALNPMIAALRERPEDFDLNRGWLTHFPSGHRFKFDRAGNV
                     SLRANCDCALLSVRQEEGQELWQAFESWRANYWRPIEINREFAAHFRRPNVAQRMFRW
                     ILRRIRAVLLDPSLDAPSADANAEAGRIAHV"
     gene            19716..>20223
                     /locus_tag="VKU03_03960"
     CDS             19716..>20223
                     /locus_tag="VKU03_03960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70447.1"
                     /translation="MSVAYSSSSSLARETTAYGGFADAIGGIATIVLAIVGLAGVKPE
                     ALVSIATIVFGAALLIQGGAMLSEFAQVEMTSADASITMSGGGISALFLVGVAGVVLG
                     VLALIGIHAPILTAVAAIAFGAALLISSSAVWRLLTSPSIAARFEGRTPIVRVVASEM
                     AAGSTGVQA"
BASE COUNT         3339 a         6635 c         6696 g         3553 t
ORIGIN      
        1 cccctccacc ggccttcggc cggttcccct cccccgttcc gcttacgctt cacgggggag
       61 gatgcgccgc tttagcgccg cttcttctcg tacggatttg tcgacgtgcg caggctgagc
      121 cggatcggcg cgcccggcag atcgaatgtc tcgcgcaagc cgttgacgag aaatcgcttg
      181 tagctgtccg gcaaggcctg aagctggttg ccgaacagga cgaaatgggg cggccgcgcc
      241 ttgggctgcg tcatgtatcg aatcttgatg cgccgtccgg cgaccgccgg cggcggcgtg
      301 gcgtcgacca ccggcccgag ccagcggttg agccgtccgg tggcgatccg cttgctccag
      361 atttgctcgg tcgcgatgac cgcctccatg agccggtcga catgcgcgcc ggtcaggccg
      421 gaaaccggga cgacccgcgc gccgcgcaat tgcggcagca gccggtcggc ctcctcgcgc
      481 aaagtcttcg ccgcctggct cttgtcctgc accaaatccc atttgttgag gccgatcacc
      541 agcgccctgc cctcgcgctc gacgagatcg gccaaggtca gatcctgcct ttcgaacggg
      601 atcgtggcat cgagaagcag caccacgact tcggcgaagc gcacggcgcg caaggcgtcg
      661 gcggcggcga gcttctcgag cttctcgacg acgcgggcgc gccgccggag accggccgtg
      721 tcgaacagtt tcagccggcg gccgcgccaa accgtctcga cgccgatcga atcgcgggtc
      781 aggcccggct cgggcccggt cagcaggcgg tcttcgccga gaatgcggtt gagcagcgtc
      841 gatttgccgg cattgggccg gccgagcacg gcgatgcgca gcggcttgga gagatcgacc
      901 tcgctgccgt cctcctcgtc gccgagttcg agcgtcggcg cctcggctcc gctctccggc
      961 tcgcgcgcct cggacggcag gaccgccgcc agcgcgtcgt agagatcgcc caagccctcg
     1021 ccatgctcgg ccgagagcgg cagcggctcg ccaaggccga gactgaacgc ctcgtaagcc
     1081 cctgcctcac cggctttgcc ttccgccttg ttggcgacga gcaggatcgg cttgccggcg
     1141 cgccggatca attgcgcgaa atgccggtcg gtcggggtaa gcccggcccg ggcgtcgatc
     1201 aggaacagga tggcgtcgca atcggcgagc gcggcctcgg tctgggcccg catgcggccg
     1261 gccatggtcg cggcttctcc ctcttcgagg ccagcagtgt cgatgaccgt gaaagaaaga
     1321 tcgccgagcc gcgcctcgcc ctcgcgccgg tcgcgggtga cgccgggcgc gtcgtcgacc
     1381 agagccagct tcctgccgac cagacggttg aacaaagtcg acttgccgac attcggccgg
     1441 ccgataatcg cgaccttggc gctcacgtgg atcgatccta acgcttagcg ggcgcgtcgg
     1501 gcgctcactt ggccgcgggc tttcccgacc ggacgaggcc gagcatcatc tcggcgttct
     1561 gccgggccgc gggcggcgat tcgggatcgg cggcgatcat atcgagccat ttgccggcgc
     1621 cgtcgaaatc gcccgccgcc aaagaggccg cgccgagcag ctcgcgcgcg gtcgagcgaa
     1681 acgcgccggt cggcgccgcc agaccttcaa gcgctttctt agcttgctcg gcttggccat
     1741 tgtccaagcg gagcgcagcg gcgcgcaacc gagccgcttc ccggaacagc gggccgatcg
     1801 attcatcgtc cgagagcttg tcataggcgg cgatggcgcg attggggtcg cttttggcca
     1861 agctcgccgc gtcggccatg cgcgccagga tcgcgtagcc cttgggcgcg tcggccgcga
     1921 gcttggcgaa gtcggcgctc gcctcgccgc tcttgccgga gcgttcaaga gaaagggcgt
     1981 cttcgaaccg cgcgccggcg gcttgcgcgg cctcaatgcg ccgccactcg tagaagcgcc
     2041 agccggcggt cgccagcagg agcagaatga cggccgcgat gatgtagttc tgatacttct
     2101 tccagaattc gacggcctgg tcgcggcgga cctcttcgtc gacttcgcga aaaatgtcgc
     2161 tcatctcaag acttcttgcg gcttttggcg ccgctccctg aattcgcggc aggcgttagc
     2221 atggccgccg cgggaaagcg agggctcagg acgcgccggc gcgccgcgtc gtctccgccg
     2281 tttttttccg cgtcttgcca tcatgaagtg cggcggctcg gcctcctggt tgataggcca
     2341 gccggaagac cagcaagaga aaaggggtgt agctccgtga aaacgcttcg acttggctta
     2401 ttggtcggct ccttggcctt cggccttgcc gcctgcacca cgacccagga gcagcttggc
     2461 ggcgcgggga ccggcgtcgt cgccggcgcg gcggtggccg ggccggtcgg cgcggtggtc
     2521 ggcggcgtcg ccggcgcgat caccggtcct tcagtcgcgc acgcggccgg cgcgccgcat
     2581 catcatcgcc actattattg gcggcatggc catcgctact acacgtatta ctgatcgccg
     2641 cggcgccccg gccgccttgg ccctgcgtgg cgcgagtggc acaatcgctc gatgctgcgc
     2701 ctcctgctgc tgcgccacgc caaagccgtc ccgcatgatc cggcgcgcga tcatggccgc
     2761 gcgctggccg agcgcggccg cgccgacgcc aagcggcttg gcaagcttct ggcgggggaa
     2821 agccgagcga tcgaagcggc cgttcattcc ggggcgcggc gggccgagga gacggcgctg
     2881 atcgcgctcg gcgaattgcc gaaaggcgtt ccggtctcgg cggagccgcg cctctacgag
     2941 gcgacgccgg cgggggtgat cgccgccttg cgcgacttgc cggacgcggc gacgaccatt
     3001 ctcgtcgtcg gccacaatcc cagcctcgcc gaggccgcgt tgcgcctagc cggaagcgga
     3061 gaccaggcgg cgctggcgcg aatggcggcg aaatatccga cctgcggctt ggcgacgatc
     3121 gatttcgacg tcgccgattg gaaggagatt tcggccggcg gcggccggct cgtcgccttt
     3181 ctggccccgg ccggccttgg cggatccgac tagctctttc gggctgggaa tagacgccgc
     3241 cagcctctac atagaggcgc aagcttccgt ccggcccgcc tttgtcgctc ttccacaatt
     3301 tcatcgatga agccggcgcc gcggcgcgca gcttcgccga ctatctcgcc ggcggcgctc
     3361 tgcggctcgg cgtcaccggc ctttcccgcg ccggcaagac ggtcttcatc accgccttga
     3421 tcaacaacct cgtcgccggc gtgcggctgc cggtgctcag cgcctctgcg gaggggcgca
     3481 tcgcccgcgc caggctcgag ccgcagccgg acgacgcggt gccgcgcttc gcctacgagg
     3541 atcatctggc ggcgctttcc gggcaggaac gccgctggcc ggaatcgacg cggcggattt
     3601 ccgaactgag ggtcacgatc gagttcgagc gcgcccatgg ctggggacgc cgggcgtcga
     3661 ccctgacgct cgatatcgtc gactatcccg gcgaatggct cctggatttg ccgctgctcg
     3721 acaagagcta cgcgcaatgg tcgcgcgaga cgttcgaagc cagcaatatg ccggcgcgcc
     3781 ggccgctcgc cgccgactgg ctggccttcg ccggcgctct cgatccatcc gccgccgccg
     3841 aagaagacga agcgcgcaag agcgccgctt tgttcaccgc ctatctgcgc aaggcgcgcg
     3901 aggcgaccta tgcgctctcg accttgccgc caggccgctt cctgatgccg ggcgatctcg
     3961 aaggctcgcc ggctttgacc ttcgcgccgc tgcgcgtcga aaccggcgag gcctattcgc
     4021 cgcattcgct cgcggcgatg atggaaaggc gctacgaatc ctacaaatcg catgtcgcgc
     4081 ggccgttctt tcgcgaccac ttcgcgaggc tcgaccgcca gatcgtcctg attgacgcgc
     4141 tctcggcgct gaattccggg ccggcggcgg tgcgcgatct cgaacacgcc ttgttcgacg
     4201 tgctccgggc gtttcgcgcc ggccgcgcct cgctgctctc gacgattttt cgcccgcgca
     4261 tcgatcgcat tctcttcgct gcgaccaagg ccgatcatct tcatcatgcg agccacgacc
     4321 ggctcgagaa cattctccgc ttcttgaccg ccaaagcgat cgcccgcgcc gaaggcctcg
     4381 gcgccgcgat cgacgtgatc gcgctcgccg cgatccgcgc gacgcgcgag gcgaaaatcc
     4441 gccaaagcgg cgagacgctc gatgcgatca tcgggacgcc cgagcgcggc gagcggctcg
     4501 gcggcgaaat cttcgacggg gtcagcgagg cggcgatctt tcccggccaa ttgccggaca
     4561 atccggcggc ggtctttgac ggcgacgcga tcggccgcag cgaggcggaa agcgactggc
     4621 gctttctgcg cttccgcccg ccgaaagtcg cgttcggcga ggcgccgccc catatccgtc
     4681 tcgaccgcgc gctgcaattc ctgatcggag atcgcctggc gtgaacgaga gccgcaagaa
     4741 gagcgacgag gccgccgaaa cccggccgcg gccgcgcgcc tttcggctcg acgatgcgcg
     4801 cgtcgccgtc gccgattccg agacgacgcc gcgccacgcc ggaatcgtca tcgagccgca
     4861 ggccgcgccg attcccgacc gcgcgcgcga gccgccgatc gaagagggcg agcgggagat
     4921 cgaagaggcg cagcgggccg gcatggcgcc ggcctggcgg ccgaagctcg cgacgctctt
     4981 ctggtcaggg cttggcggcc tcgtgtcgct cggaattggc ctctgggcca accagctcat
     5041 cgagaccctg ttccagaagg cgcaggccct cggctggatc gggctggcgc tcggcgcgat
     5101 gacgctcttc gcggctttcg cgctggttct tcgcgaggtc gccgcgatct tgcgccagag
     5161 cgcgatcgcc aagctgcacg ccgctctcgc cttggcgagg gcgaatgacg atcgcgacgc
     5221 cgcgcgcaag cttacgatgc ggctctgcgc gctctacgaa aagcgccctg agacagcgcg
     5281 cggccgcgct gagatcgccg acgccgcggt ccagatcatt gacgggcgcg acctcatcga
     5341 cattgccgaa cgggcgctgc tgcggccgct cgatgcgaaa gcgcaaagcg agatcgcgac
     5401 ggccgccaag cgcgtatcca tggtgacggc gataagtccc cgcgcgatcc tcgatatgct
     5461 cttcgtcgtc gcgcagatca tccgcctcat ccgccgcatc gccgaaatct atggcggccg
     5521 gcccggtcta ttcggttttt tcaagctcgc ccgctccgtc gccgcgcatg tcgcgatcac
     5581 cgggggcatg gcggtcggcg acagcttgtt gcagcaactc gtgggtcacg gcatcgcctc
     5641 gaaactgtcg gcgcggctcg gcgagggcgt gctcaacggc ctgctgacgg cgcgcgtcgg
     5701 cctgtcggct ttggccgtct gccgcccggc gccgttcggc gtcgaaaagc cgccgggcgt
     5761 cgccgatgtc gcgccgtttc tgctgaccgg cggcaaggag cgccgaactt gatcggaacc
     5821 tcgccaggcg aggacggttg agggaggcgc gcgccgccgc gcgatcccgg gaagcccaca
     5881 ttgtcgctga cgccctcgcc cgacgacatc cgcgccaagg cttccgatgc gccgatcaag
     5941 aatggcggct tgcgcttcgg cccgctcggg cgccattgcg tccatctctg cgtcgacatg
     6001 cagcgcatgt tcgcggaaga gaccgtgtgg cgcacgccct ggctgcgtcg cgtcctgccg
     6061 aacatcctgc gcctcgtcga atcgcggccg gccgcgacgg tcttcacgcg tttcattccg
     6121 gccgaccggc ccggcgaagg gcccggcgcg tggcggcgtt attatgaacg ctggtcgtcc
     6181 atgacgctgc aaaatctcag cagggacatg gtcgagcttg tgccggaatt ggcgatgttc
     6241 gttcctcccg ccgcaatatt cgacaagcgc gtctattcgc cgtggcgcgg cggcgcgctg
     6301 cacggcgacc tgctgcgccg ctcgatcgat acgcttctcg tgtccggcgg cgaaaccgat
     6361 gtctgcgtgc tctcgaccgt gctcggcgcg atcgattacg gctatcgcgt cgtcatcgtc
     6421 acggacgcgc tatgcagctc ttcggacgaa acccacgacg cgctgctggc gctttacgaa
     6481 acgcgctacg ggcagcagat cgaagccgtg acggcggacg aagcgatcga gagctggaag
     6541 tgacgcgtgt cattcccgcg aaagcggaaa tgcagtgagg cgaatccttc gcgcgccgcg
     6601 catctgggtc gccggatttt tcgtcgaggc ggacatccga tttcctcagc gatcctgctg
     6661 cagaccctga ttggtcgtgt tctgcttgat gttggcctcc tggccgcgct tctcagcgcc
     6721 cgcgcgagac tcgcggcggc cttgttcggc cggcgcgcgc ttgtcgtcgc caggcccttt
     6781 gggactttgg ttggccggcg gcgccggcgg cgttttatgg ctcattgcgg gctccacgta
     6841 ccagccgcgt tcagatgatc ggcttgccgc cggtgacggc gatggtggcg ccggaaatgt
     6901 agctcgcctc gtcgctcgcc agcagcacgt aggccggcgc cacttcgcag ggctgagccg
     6961 gccgcttcat gggaacctgc tcgccgaaat gcgcgacctt ctccttcggc atggtcgacg
     7021 ggatcagcgg cgtccagacc ggacccggcg cgaccgcgtt cacgcggatc cctttctccg
     7081 ccagcatttg cgccaggccg ccggtgaaat tctggatcgc ccccttggtc gtcgcatagg
     7141 cgaggaggtg ggggctcggc gcgtcggcgt tgaccgaagc ggtattgatg atcgaggcgc
     7201 cctctttcat gtgcggaacc gccgccttgg cgatgtagaa catcgccgag atgttggtcg
     7261 cgaaagtctt gtcccattcc tcgtcggaaa tatccccgat cgacttgaag ctcatctgat
     7321 gggcggcgtt gttgacgagc acgtcgatgc ggccgaacgc gccgagcgcc ttttcgacca
     7381 gcttgcggca atgatcgggc gaagagacgt cgccaggaat gagaaggcat ttccgcccgg
     7441 cctcctcgac caggcgcttc gtctcggccg cgtcctcctc ctcgttgaga taggagaccg
     7501 ccacgtcggc gccttcgcgc gcgaaagcca aggcgacggc gcggccgatg ccggaatccg
     7561 cgcccgtgat gagcgtcttc ttgtccttga gcttgccggc gccgcgatac gtgtcctcgc
     7621 cgtaatcggg ctgcggattc atcttcgcgc tatgcccggg aacccgctgc tgttgctcgg
     7681 aaaagggcgg ttttggaaac ggcttattca tggcggacgc tcgctctcaa aatagagaat
     7741 cgagcggctt aacgtctcgc tttagcgaag gtttcggccg cggccgaatt tgcccccgcg
     7801 ccggtgaggt gcaggcgagc ggcaatcgcg ctatggtttc agcgtcggct cgggggcgag
     7861 atcatgctga gggcgtcggg aaggcgcggc gaaatcacgc tcgcggcgga ggacggccgc
     7921 atcgtctcgg tgctgaatgg ggggctcttc gccttcacgg tcggcgagcg cgcctaccag
     7981 atcgcgcgca agggactgtt ctcgccaact tatgagctcc ggcgcgagga cagccttctc
     8041 gcctcggcgg cgcaagcagc cttcgtcaac cgctacgccg tcgagtgcgc gggaaaagcc
     8101 tggacgttga aggccgccgg cctcgccgcg cggaaatttg cgctgctcga cggcaaaatc
     8161 gcggtcggcg cgatcgcgcc gacttccctc aatccgtatc gcgaaacgat cgtcgacctg
     8221 ccggaggcca ttccggtcga ggcgcagatt ttcctgacct ggatcgtcgc ccgccattgg
     8281 gatcaggact gatcggttat tctcgcttgc tgcgggaatg acagcgttca cgcggcgtcg
     8341 gcggtgaccc ggttgcggcc ttccgctttc gaccggtaga gcgcgcggtc ggcgcgctcg
     8401 taaagcgcgt cggcctcggc gtcgcggccg cgctcggcaa ggccgatcga gaccgtgatc
     8461 ggaatggtca gcccggtctt gtcgatgacg aagggcgcct cctcgatcga ggcgcgggcg
     8521 cgctcggcga ctttcccggc gacgtcgagc ggcacgttgg gcatgacgac gacgaattcc
     8581 tcgccgccca tccggcacag caggtcgccg ccgcggatga tgcggcggat gcggtcggcg
     8641 aagcctttca gcacttcgtc gcccgcctga tggccatacg tgtcgttgac ctgcttgaaa
     8701 tggtcgatgt cgagaatcat cagcgacacc ggcttatggc gcgccctggc ctgctcaagc
     8761 gtcgcggcga ggtgattttc gaggaagcgg cgattgttga ggccggtcag cggatcgacg
     8821 atggaaagct cgatcgagtg ctgcaccttt tcgcgcaagc gatcggcgta gcgcttgccg
     8881 cgcaggagaa tgcgggcgcg ggcgagcaat tcgttgcggt cgattggccg cgacagatag
     8941 tcgttgacgc cgagctccag gccgcgcagc acgcggtcgc ggtcctccat ttcggcgatg
     9001 accaggatcg gcaggtggcg ggtgcgctcg agcgccctga tctggctgca gaatctcagc
     9061 ccgtcgtaat tggcgagacc gaggctgacg acgaagagat cgtaattgtc ctcggcggcc
     9121 ttgaacaaag cctcctgggc atcggcttcg gcgacgactt catgccgggc gccgagcgtc
     9181 tggcagatgc gctcgcgcga actctcgcga tcgtcgacca gcaggatgcg gccgggccgg
     9241 tcgtcctccg ccagcagcgc ggcgttctcg tcctcgacgc gcagagccaa cgtcgtattg
     9301 gcccgattgc gcaattcgtc gacgaccact ttgaggcgga ccagcgagcg cacgcgcgcc
     9361 atcagcgcga cttcgtcgat cggcttggtc aggaaatcgt cggcgcccgc ttcgaggccg
     9421 cgcaagcggt cggccggctg atcgagcgcg gtgatcagca ccaccgccag gtgcgccgtc
     9481 gccgggttgg tcttgatccg gcggcagact tcgaacccgt ccatgcccgg catcatcacg
     9541 tccagcagga cgatgtcgca caaatcctgc tcgcatttct gcaaggcctc gcggccgctg
     9601 gacgccgtgg ccacgtcgaa atattcggcc gtcaatttcg cttcgagcaa tttgacgttc
     9661 ggcgcgatgt cgtcgacgac gagaatgcgg gcggtcatcg tctcaccgag gtcctagaaa
     9721 actatccaca gtctcgaaga acttcgccac cgatatcggc ttggagagat aggcttcgca
     9781 gccgccttgc cggattttct cttcgtcgcc tttcatagcg aaagcagtga ccgcgatgac
     9841 cggaatggcg cgcagatcgt catcgtcctt cagccattgg gtcacttgca ggccggacac
     9901 ttcgggaagc tgaatatcca tcaagatcag gtccggccgg tgcttgcggg caaggtccat
     9961 cgcctcgacg ccctgccgcg tctgcagcac ccggcagccg cgggtctcca acagatcgtt
    10021 gaagagcttc atgttgagct cgttgtcttc tacgatcaga acggttttcg acatcggcgt
    10081 ttccgagcgc ggcgttaatt tttcgctaaa gcccatttac ccggcgggca ttgggtaaga
    10141 ctatctctga gaaatttacg aatggaaaat tgaatcgtgt acacaaggcc taaatcgcga
    10201 tcttccacgg acgaggagga ggcgcaaggc ttagccatcg acgcgctcgc ctggctcgcc
    10261 gaggatttgg agcgcttgca gcgttttctg gctgtttcgg ggcttggtcc gcaaaatttg
    10321 cgctccgccg cgtcagagcc cggctttctt ggcgccgttc tcgattactt ggccgccaat
    10381 gaacacttgc tgatcgcttt tgcaacccac agaaatgtca cgcccgagtc ggtgatgcgc
    10441 ggccgggagc ggttgagcgg agggcgagcg caagacgaag aatgagcgcc ggcccctgga
    10501 tatgtcgcga ttgcgtcagc agcttttcga gcgccgctgc agcagtccgg cgttgttcgc
    10561 aatgcggttc gccgcgcata gtcgccgatc cggttcacaa tgaattgacc atagcgcatg
    10621 tcgattgcga cgccttctat gcggcgatcg agaagcgcga caatccggcc ctgcgcgacc
    10681 gtccggtgat cgtcggcggc ggcggacggc gcggcgtcgt ctcgacctgc tgctacatcg
    10741 cgcgcatttc cggcgtgcgc tcggccatgc cgatgtatca ggcgcgccgc ctctgtcccg
    10801 aggcgaccgt gatcccgccc gacatgggca aatacgccga ggtcggccgg cagatccggg
    10861 cgatgatgca gcgcctgacg ccgctggtcg agccgctgtc gatcgacgaa gcctttctcg
    10921 acctttcggg ctgcgaaagg ctgcatggcg cgagcgccgc ggcgacgctc gcgcgtttcg
    10981 ccaaggacgt ggagaccaag gtcggcgtca cggtgtcgat cggcctcagc tattgcaagt
    11041 tcctcgccaa attcgcatcc gacttggata agccgcgcgg cttctccatc gtcgcgcgcg
    11101 atcaagcgct ggcgctgctc gcgccgcagc cgatcggcag gatctggggc gtcggccgcg
    11161 tcgccgaggc gcggctggtc aaagcgggcc tcagcacgat cggcgacgtg cagcggcgca
    11221 gcgagatcga gatgattcgc gaatgcggcc cagacggcgc gcggctttgg cgcctcgcgc
    11281 atggcatcga cgagcggcgc gtctcgccgg agcgcgagac caagagcatt tccgccgaga
    11341 cgacgttcga tgtcgacatt gccgacaagg ccgagctcga gcgcgtcttg ctccggctct
    11401 gcgagcgcgt cgcgctgcgg ctcaagcgcg agggcctcgc cgcgcggagc gtgacgctga
    11461 agctgcgcct gccggatttc aagctgcgca cccgcgcccg ctccggcgcg gcgccgacgc
    11521 aactcgcgag ccgcctcttc gccgtcgcgc gcgatctcct cgcccgcgag gccgatggcg
    11581 cggccttccg cctgatcggc gtcgccgccg ccgatttcgc cccggccgaa gaggccgatc
    11641 gcggcgatct catcgacaag gacgtcggca aggagaaagc gcgcgagcag gcgatcgacg
    11701 cgctgcggga gaagttcggc gcgagcgcgg tggtgcgcgc tcctcttctc cccgtgaaac
    11761 ggggagaagg tgtcgccgaa ggcgacggat gaggggctgc gctgaacttg atgataagcg
    11821 attcgtagag cgcgtacctt ctggacctga gcagcctctc atccggcggc ctcagaggga
    11881 cgaggaagag cccggcatgc ccttaggatc gcgtgcggca tgcctgattg catggataac
    11941 aatttccgcc tcaacaggct catagaagat gacgtaagga taaggagagg cgaccaagcg
    12001 gcgaatggtc gggtcttcag tcggcgctcc catatgcggg tggagcggca aaagagcgat
    12061 agcggatttg atccgggatt ggattcgccg agcgccttgg ggcgaatgcg ccgcgacgta
    12121 atcgaagatc gattcgagat cggcaagcgc cggcagcgtg tagcggattc tcagagcccg
    12181 tatttgcgcc agattgccgc gatctgctcg tctgtcgcaa attcgccgcg ggcggcggcg
    12241 gccttggacg cggctatggc tgcgcgttcg tccgaagtca gcaggatcgg atcgagatcg
    12301 tcggcgcccg caagctggag cacgaccctc gcaatgtcgt cctgatcaga ctcgggcagc
    12361 cgtttggcgg cttcgagcgc tcgttcgaga agcttggtca tagagccgag gatacagttt
    12421 ctttggactg gagcaaaggg accgtatcgc gccaacaatt tcggctcctg cgcagcccct
    12481 tatccggccc ttcgggccac cttctccccg cttggcgggg agaagggagc gccgcgttgg
    12541 tactttcagc gacgcggctc gatccgcgcg aggccgccca tataggggcg caagacgtcc
    12601 ggcacggcca ccgagccgtc ggaattttgg tagttctcca gcacggcgac gagggcgcgg
    12661 ccgacggcga cgccagagcc gttgagcgtg tggacgaagc gggtctgctt ggcgtcggcg
    12721 gccttgcagc gggcgttcat gcggcgggcc tggaaatcgc cacagaccga gcaggaggaa
    12781 atctcgcgga aacgcccctg ccctggcagc cagacttcga tgtcgtaggt tttccggctg
    12841 gcgaagccca tatcgccggt gcagagcgcg acggtgcggt aatgcaggcc gaggcgcttc
    12901 aagacctcct cggcgcaggc ggtcatgcgc tcgtgctcgg cgcgcgattg ctcgggcgtc
    12961 gtgatcgaga cgagctcgac cttgtagaac tggtgctggc ggatcatgcc gcgcgtatcc
    13021 ttgcccgccg cgcccgcctc ggcgcggaag caataggtgc cggcagtgta gcggagcggc
    13081 agcttggctt cgtcgacgat gctctcgcga acaagattgg tgagcgggac ttcggcggtc
    13141 gggatgagcc aatatttatt tactgattga aaagcctcaa gcgaagtatt cgctctgtcc
    13201 caagcttcat cgtaatccgg tttgcacatc gccgaaaatt gatcgtccac aaacttcggc
    13261 aactgcgccg taccgaacat cgtctgatcg cgcacgagca gcggcggcgc tacctcttcg
    13321 tagccatgct ccgtcgtgtg catatccagc atgaactgcg ccagcgcccg ctccagccgc
    13381 gccagcgcgc ctttcagcac gacgaatcgc gcgccggaca ttttcgccgc gctttcgaaa
    13441 tccatcaatc cgagcgtctc gccgatatcg aaatgttctc tcggcgcgaa gccgtccgcg
    13501 aagcgcggcg cctcgccaag acggcggatt tcgacattct cagtctcgtc cttgccgtcc
    13561 ggagcatcgg cggccggaat gttcggaatc gcggcgagct cgttctccaa cgcgctcagc
    13621 gcggccttct ccgccgcctc cagctccggc attctcgtct tgaggtcggc gacttccttc
    13681 ttgagcgcct cggccagcgc gaaatccttc ttccccatcg cctggccgat ctctttcgag
    13741 acggcgttgc gccgctcctg cgccttctgc aattcgagaa tcgcggcgcg gcgcttgtcg
    13801 tcgagggcga tcagagcgcc ggcttgcggc ggcaagccgc ggcgggcgag ccccaaatcg
    13861 aacgcctccg gatcgtcgcg gatgagcttg atgtcgtgca tggcccctaa gccgcttttg
    13921 cgcgccggcc taaaatccgg tcgccgaaga cgttgaatgt gagccccgcc attaccagag
    13981 cggcgcctgt cagttcaatc gcgcttaagg gctcgccgaa aaccaccgcg ccggagacca
    14041 tgccgacgat cgggatcaac agcgcgaaag gcgcggcctc ggcggccgaa taaagcgcga
    14101 gcaggcgcgc ccaaagaccg taggagatca ccgtcgcgcc ataggcgagc aaggcgacgc
    14161 agaggacgag ggtcaggctc ggatgagcga gcgcggcgag ccgccctggc ccatcaaagg
    14221 ccaacgacag gaggaccagc ggcgcgggga cggcgagcgc cgcccagacg acaaaagcca
    14281 gcatgtcgat cttgccggcg atcttgccga tcagattgcc gatcccccag aaggcggccg
    14341 cgagcagaac gaggagaaac gggccgagac ttgcgccgtg cagccgctcc gagccgatcg
    14401 ccgcgatgcc ggcgagcgca acgcccgcgc cgaccaattg cgccggccgc ggcctctcgc
    14461 cgagccacgc ccaggcgaga aagatggtga tgaagacctg caattggatg acgagcgaag
    14521 cgagtccgac cggcatgccg aggccgatgg cgacgaacag gacgccgaac tggccgacgc
    14581 cgatcgtcag gccgtatagc gtcgtcagcc tgaacgaggc tttcggcggc ttgatgaaaa
    14641 gcacggccgg aaaggccgcg aaggcgaagc gcaaggcggt cagcaggaaa ggcgtcgttt
    14701 cgcgaagccc gatcttgatc gcgatgaagg cgaggccaaa aaacacagcg gcgatgatcg
    14761 ccgacaaagc atcgcggaga gtcatgaggg cgtcgggaag gtcgagagga aaaaggatcg
    14821 gattagcgcg ggatgaaaat cgcccgtcat tgcgaggagc gaagcgacga aacaatccat
    14881 tcgtagggcg gcgctctatg agcggattgc ttcgctcccc tcgcaatgac ggagtcgcgg
    14941 cggcgtgtca ctttgacgat tgggccgcca atcgtttctt ctccaccatc tggacggcga
    15001 aaatcgcgcc ctcgtaaagc agcagcatcg gcacggcgag cgagcacatg ctgatcacgt
    15061 cgggcggcgt gaggattgcc gcgaggccgg tcacgatcac gatcgcgtaa cgccgcttgg
    15121 agcgcagata ggccgaatcg atgatgccga cttgtcccag cagcgtcagg atcaccggca
    15181 attggaaggc gacgccgaag gcgaagatca aggtcatgat cagcgagagg tattcgttga
    15241 ccttgggcag caattcgatc gtggcttcgc cgggccgatt gacctgctgc aaggagaccg
    15301 agaagcggat cagcagcggc atgaccgcga aatagacgag cagcgcgccg agcacgaaga
    15361 agatcggcgt cgcgattagg tacggcaaaa aggcctggcg ctcgctgcga tagaggccgg
    15421 gcgcgacgaa cttgtaaatc tgcaccgcga tgatcggaaa ggcgatgacc gccgccccga
    15481 acatgctgag cttcatattg gtgaagaatt gttcgaggaa atgcgtcgcg atcagcttgg
    15541 cgttctcggc gccgacgata tgcacatagg gcagcgtcag gatgttgtag atcgtgcgcg
    15601 agaagtagaa gcagatgatg aaggcgacgg cgaaggcgag cagcgaacgg atcagccgcg
    15661 cgcgcagctc gatcagatga tccatcagcg gcgccttggt cgcctcgata tcggcttcgg
    15721 tcatggcgcg cggccctcgc gcagggcgct ttcggcgaat tcggcttgca acggcggcgc
    15781 ggtctcaacc ggcggcgttt gcggcgcgct atgcgccgtc gcgccgttcg gcttcgcctc
    15841 gaacggcgcc tccgcgacgg cgggcttcgc cgcctcggtc ttggcggcct cgttcatcgc
    15901 ctcgagcgct ttcttcacat cgtcgagatc cgcttcgctc cgcgcctcgc ccgccgccgg
    15961 ctcgaccggc ttttcctcag ccgcgggctt tgcgccttcg atcgcggccg gctggtcatg
    16021 ctgggtgagc gtcgctggat cgaacgacac gtcgacgccc gtcacctcga ccttggcggc
    16081 ctcgttcatc gccgccagct ccttcttcac gtcttcgagc tccgcctcgc gcatcgcctc
    16141 gttgaattgc gcttggaact cgttggccat gcggcgcatg cggccgacca cctggccgac
    16201 cgtgcgcatc acgcgcggca attccttcgg cccgatgacg atgagcgcga cgacgccgac
    16261 gatgaaaatc ttggaaggat cgaagatatc gaacatgctg cttcgtcggc ccgggcggga
    16321 tcgcgcgccg cgctaggccc gcctggccga agcttggggc gtcgctcgtt tcttaaaggc
    16381 tcaaccgacc ttacgttggt ccgtcgtcgt ctcgaccggc tggctctggc gactttcaat
    16441 cgccccggcc ggctgggccg gcggcggcgc ggcggcctcg tcgtcgttca gacctttctt
    16501 gaacgatttg atgcctttgg cgaaatcgcc catgatctcg gaaatcttgc ctctgccgcc
    16561 gaacagcaac aaagcgatga ccgcaaaaag cagcagatgc tgcatgctga atcccatcac
    16621 gaaaccctcc atcgattcga tcgcctgcgg ctgcggccga atgcgacttg caaaccgttg
    16681 acaactgctc cgaacttaag cctcgaaagg ggcaaaaaca agcgcgggcg ctggagcaag
    16741 cccttctccc cgcaaagcgg gcagaagggg attcccctcc gcgccgaatg gtgtagagaa
    16801 gccgccccga aatcgccgac gagtcccgcc ttgaacgatc gcgtcgaaag cgccctcaac
    16861 gccgccctgg actggctcct gcgcaaccaa aagcagggcg gatactgggt cggcatggtc
    16921 gagtccaatt cctgcatgga ggcggaatgg ctgctcgcct cgcacattct ggacgtcgaa
    16981 ttgccgatgc gggacggctt tatccggacg ctttttcacc gccagaggcc ggacggcgcc
    17041 tgggatgtgt ttccaggcgc gccgaaaggc gacatcaatt cgacggtcga ggtctatgcg
    17101 gcgctgattg cgatgggcga aagccccgac cggccggagc tcgccaaagc gcgcgccttc
    17161 atcctcagcc aaggcggtct caagacggtc cgggtgttca cccgctactg gctggcgctg
    17221 ctcggcgtct ggccctggac ggatacgccc aatctgccgc ctgagtttat ccgctttcct
    17281 ctgtggttcc cgttcaacat ctataatttc gcgcaatggg cgcgcgcgac catggtcccg
    17341 ctggcggtgt tgagcgcccg ccggccggtc ttccccctgc ccggcggcgc ccgcctcgac
    17401 gagctctttc ccgaggggcg ggagaagttc gatttctccg cgccggtgaa aaaagccgag
    17461 ccttggtcgc tggaggcctt cttcttgcgc gtcgaccggg cgctgcattg gctgcagacc
    17521 cggcggctaa ttcccggccg cgagaacgcg atcaagctgt gcctggaatg gatgatccgc
    17581 caccaggacg ccgacggcgc ctggggcggc atccagccgc cttggattta cggcctgatg
    17641 gccttgcgca acgagggcta cgccctctcc cacccatgca tcgtcaaggg gctcggcgcg
    17701 ctgtctgccc attggaccta tgagcgtcat ggcgggatcg cggtccaggc gagcgagtcg
    17761 ccggtttggg acacgctgtt ctgtcttctg gctatggccg aaaccggctg gtcgccggcg
    17821 gaacggccgg cgatgcggga cgcccttgtc tatattctcg acaatcaaat tctcgagcct
    17881 ggcgattggt cgctgacggt caagggagtc ccgccgggcg gctgggcgtt cgagcgcgcc
    17941 aatctgcgct atcccgacgt ggacgatacc gccctcgcct tgctggtcct cgccaagctg
    18001 cgcggcaaag cgggcgatct cgacggccgc gtcgaggcgg cgatcgagcg cggccttgcc
    18061 tggaccttcg gcatgcagag ccgcaatggc ggctgggcgg ctttcgatcg cgacaacgac
    18121 aaggcgatca tctcgaccat cccattctgc aatttcggcg aggcgctcga cccgcccagc
    18181 gtcgatgtca ccgctcatgt gatcgaagcc ttggccgagc tcggcatgag caagaagcgg
    18241 ccggaaatcg cgcgggcgct cgccttcatc gagaaggagc aggagccgga aggctcatgg
    18301 ttcggccgct ggggctgcaa tcacatttac ggcgtcggcg cggtgctgcc ggcgctgaag
    18361 gcgatcggct gcgacatgag cgccgcccat atccgcaagg cggccgactg gctcgtcgac
    18421 aagcagaacg ccgatggcgg ctggggcgaa agctgcgaat cctacatgga cgcgtccaag
    18481 atcggccgcg gcccgtcgac gccgacccag accgcctggg cgatcatggc gctgcaggcg
    18541 gcgggcggcc acgagccggc gatcgcgcgc ggcctcgacc acctgatcgc gacgcaagac
    18601 gcgggcggaa cttgggacga agccttctac accgcgaccg gctttcccgg atacggcgtc
    18661 ggcgcgcgca ccgaattgcg cgatcccaag ctcgccgaaa agctcggcca aggcgccgag
    18721 ctgtcgcgcg gcttcatgat ctctttcaac ctgtaccggc actattttcc ggcgatcgcg
    18781 ctggcgcggc gcctccgtag cgaggcgcaa gcgttggacg cgcactcatc tgctctgcgc
    18841 gcgccggtcg gaacttagcg acagggcggc gcggacgcat cctcccccgc ttcgcgggtg
    18901 agggttacgt accaaccgcc cgaggccgag gcgctccgat cagacgtggg cgatgcggcc
    18961 ggcctcggcg ttcgcatcgg cggacggcgc gtccagcgac ggatcgagca gaacggcgcg
    19021 gatgcggcga agaatccagc gaaacatgcg ctgcgcgacg ttcggccggc ggaaatgcgc
    19081 ggcgaattcg cgattgatct cgatcggccg ccaataattg gcgcgccaac tctcgaacgc
    19141 ctgccaaagc tcctggcctt cctcctggcg gacgctgagc aaagcacagt cgcaattggc
    19201 tcgcaggctt acattgcccg cgcgatcgaa tttgaaacga tggccgctgg gaaaatgcgt
    19261 gagccaaccg cgattgaggt cgaaatcctc ggggcgttcg cgcagagcgg cgatcatcgg
    19321 attgagcgcc agataaggca tggcatcctc ctcgcccctc gccgcgcccc aacgggagcg
    19381 gcttgccgcg gttccctccg cgtcattctg acgcatccgg cggggaggcg gcgaccttcc
    19441 gatgagcgat tgtcggccaa gctctgagcg cgcggtcggc gcggcttgac gtaacgctgg
    19501 agcgaatttc gcactgcaca aacggccgcg gcgcgagccc ggcgatcgcg caagcgcagc
    19561 gtccgaaacc ggaacattgc cgcgaaaaac aagttttttc tacgaagcgc aagaaaaggg
    19621 gcgctgcggc cgcagccgga acctgcacgt ctcggacgaa tttccttcgc agtcccgccg
    19681 gtagagcctt tgcggcgctc gttaaggagg ttcccatgtc tgttgcgtat tcttcatctt
    19741 cttctctcgc ccgcgaaacc accgcctatg gcggcttcgc tgacgcgata ggcggaatcg
    19801 ctacaatcgt cttggcgatc gtcggtctcg ccggagtaaa gcctgaagcg ctcgtgtcga
    19861 tcgcgacgat cgtgttcggc gcggcgctgc tcatccaagg cggcgccatg ctcagcgagt
    19921 tcgcccaggt cgaaatgact tcggccgacg ccagcatcac gatgagcggc ggcggcatat
    19981 cggccttgtt cctggtcggc gtcgccggcg tggtgctcgg cgtgctcgcc ctgatcggca
    20041 tccatgcgcc gatcctgaca gcggtcgcgg cgatcgcctt tggcgcggcg ctgctgatca
    20101 gcagctccgc tgtttggcgg ctcctgactt cgccgtcgat tgcggctcgc ttcgagggcc
    20161 gcaccccgat cgtgcgggtc gtcgcctctg agatggccgc gggctcgacc ggcgtccaag
    20221 cca
//
LOCUS       DATGJW010000133        16790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_543552, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000133 DATGJW010000000
VERSION     DATGJW010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 16790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16790
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_543552"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            146..1303
                     /locus_tag="VKU03_03965"
     CDS             146..1303
                     /locus_tag="VKU03_03965"
                     /inference="COORDINATES: protein motif:HMM:NF018434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ70448.1"
                     /translation="MARVLFLVFSGGGNLPPSLGIARALRERGHEAIFAGEPEMIPRV
                     KPTPFRAIEFADAYAQVDLYKKSPFGAVVCYLSSPAVAAEARAIIETEKPDFILVDFL
                     FFAARLAAKACERANAIMAHMALLRVAEPFRGLIDHLNGLHDEAGLAPLPSFDTLTFS
                     GEPVIVTSLAAVEQGRAALKGAGNVRYVGPALEMEAHARPLALPWDRDDPRPLALISF
                     STDPIQADPDLIQRALDALAPLDLRVVATVGRAVDRGRLAVPPNALAVEAADHDRLIA
                     AAALVVTHGGHGTVMRALKHGAPMLVLPGFIPDQAPNATLVADLGAGRILGQDSAVAA
                     IRAAAEAILRNPDYRAKARRAAGLFGRRDGAVGAADEVEKLLARKAAAKRA"
     gene            1403..2002
                     /locus_tag="VKU03_03970"
     CDS             1403..2002
                     /locus_tag="VKU03_03970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173837.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LemA family protein"
                     /protein_id="HLJ70449.1"
                     /translation="MPVILSRLRALTVLLAIGLTLSACGYNRIPTREEAAKAAWAEVQ
                     NQYQRRSDLIPNLVATVQGYAKQEKDVLTQVTEARAKATAIHVDASQLTDPAKVKQLQ
                     DAQNALSGALGRLLAVSENYPELKSNQNFLALQAQLEGTENRIAVARRDYIDAVRAYN
                     LELRTFPGVLWASTFFRSNQPMAEFAADSGAQGAPKVKF"
     gene            2115..2852
                     /locus_tag="VKU03_03975"
     CDS             2115..2852
                     /locus_tag="VKU03_03975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HLJ70450.1"
                     /translation="MALAFFLCVPAIAAELNFPPLSGRVVDQANILSPDTRGALASKL
                     KDLEDKSGIQLVVATVSSLQGQEIEPYANQLFRTWRLGEAKKNNGVLFLVAPKEHRVR
                     IEVGYGLEGTLTDATSKIIIANAVAPRFKAGDFNGGVQRGVDDIVTALTADSAEWQKR
                     PAVREEDNSSLVALLPLLLFAAIFLLVFIHTVRSRGGIGPALLYMLMSGGNSRRGDFG
                     GGGFLGGGDSFSGGGGSSGGGGASGSW"
     gene            2852..3463
                     /locus_tag="VKU03_03980"
     CDS             2852..3463
                     /locus_tag="VKU03_03980"
                     /inference="COORDINATES: protein motif:HMM:NF016424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TPM domain-containing protein"
                     /protein_id="HLJ70451.1"
                     /translation="MTVSHDDAARIEAAIRAAQARSSGQIVFVLARASANYEVMPFVW
                     SAVLALLTPWPLLDLTEISAERIYLAQLAVFIAALLVLTLPRLRLLLTPARIRRVNAH
                     RAALEQFALRGLAHNEARNGVLLYVSLAERFARIIADDAAAKAVPQKEWQALIDRLLT
                     EMKSGQTAEALIGAAARCGDLLAQSFPPRDGAARPSFHRFHVI"
     gene            complement(3545..4309)
                     /locus_tag="VKU03_03985"
     CDS             complement(3545..4309)
                     /locus_tag="VKU03_03985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012493976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HLJ70452.1"
                     /translation="MSVQRAAVAALAATGMVIYSTLGLAADASPVKAVVELFTSQGCS
                     SCPPADRILAELARDPETLAISLPVDYWDYIGWKDTLAEPVYTARQRAYARASGRGEV
                     YTPQAIINGVADAVGSDRAQIETAEAQTRKRPDVLSTPLSVSEQDGSIAISIGAAPSG
                     KSHKAGVYLIALASKRTVAIKRGENAGSTLTYSNVARAITKVGEWTGAPIVLKASLDQ
                     ARLNGADSYAVIVQEGGRSAPSAILAAAKGPQPPSL"
     gene            4563..5972
                     /locus_tag="VKU03_03990"
     CDS             4563..5972
                     /locus_tag="VKU03_03990"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanyl-D-alanine carboxypeptidase family
                     protein"
                     /protein_id="HLJ70453.1"
                     /translation="MQLGRWRAGILATLLALVAGPAFATPALVIDADSGKVLYERQAT
                     ANWYPASLTKLMTTYVALSAVREGRVTLDTPFVMSQRAASMAPSKMGFKPGTEVTLDN
                     ALKMLMVKSPNDIAVMIAEGVSGSVESFADEMNAAAAKLGMKETHFVNPNGLPDDRQV
                     TSARDMAIVARALLKEFPDEAGIFGIGALQLGNRIIPTHNGLIGRYPGADGMKTGFTC
                     AAGFNLVASATHGGKRLIAVILGAATPRERTAMAIALFERGFAETGGLAGLFSGGGSG
                     LGLVDALPSPGVGPAPDMRAEICSRRGRAALLAAEEEDMRAAAPPAVAAARTGATRGM
                     SLFAAAAASTDPEAMAAAPAPHFDPVRVFVGPKPGWTGPVAMAKPQPFEAPESASAYA
                     AGPDKEAGDSNQATSAPMALQGAVKPTKLGSLVAPHKPEAKRAHAMKQGKKIATVLTP
                     KKPAFSKVPIGKRTRSTAH"
     gene            6329..8248
                     /gene="htpG"
                     /locus_tag="VKU03_03995"
     CDS             6329..8248
                     /gene="htpG"
                     /locus_tag="VKU03_03995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HLJ70454.1"
                     /translation="MTTDVHPNADEAKTQSFEADVSRLLHLMVHSVYSDRDIFVRELV
                     SNAADACEKLRYEALAKPELIEGGSPFAITIAIDPEAKTLTFSDNGVGMSRADLAEGL
                     GTIARSGTRAFLDKLSAAESEKEGSNLIGQFGIGFYSAFMVADEVVVETRRAGSSEAW
                     RWSSDGKGAYTIAPLPLDEAPQRGSRVILRLNGASHDYAEPHRLERIVREHSGAVAVP
                     IDLIEKPGAEPRRIADGAALWAKPKSEVKPEEYAEFYRGLAGQFDEPALTIHYRAEGR
                     HEYTVLAFIPGSRPFDLFDPVRKGRNKLYVRRVLISQDTDILPGWLRFVRLVVDSADL
                     PLNVSREMIQESPVFMAIKKGVTNRIVQELSKLAESQEESDKEKFAKIWENFGAVIKE
                     GLYEDPERRDGLFKIARFATSASPKGDRTLADYVKNLRENQTAIFYLTGEDLKRIETS
                     PQLEGFRARGVEVLLLADPVDAFWVSTAAGYDGKPFKSVTQGAAEIKTIPLVEGAKSG
                     GEAPSAEVATLFAFFKQTLGEAIGEVRASERLTDSLACLVAPEFGPDRQLEKMLAAHG
                     RLSDRAKPILEVNAGHPLVASLAKRYREAADKSLVEDAAWLVFDEARLMEGETPVDAQ
                     AFAGRLRRVMEKALG"
     gene            complement(8296..9696)
                     /gene="fumC"
                     /locus_tag="VKU03_04000"
     CDS             complement(8296..9696)
                     /gene="fumC"
                     /locus_tag="VKU03_04000"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004333 - fumarate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fumarate hydratase"
                     /protein_id="HLJ70455.1"
                     /translation="MGAVRSETDSLGKVDVPADKLWGAQTQRSLEHFSIGRDLMPREM
                     IAAYAILKKAASEANHAGGRLDKERRDLIVTVCDELLEGRHEDMFPLHVWMTGSGTQF
                     NMNVNEVISNRCCQLAGTPLGGKSPVHPNDHVNMSQSSNDNFPSAMYIAAAVNTRRRL
                     LPSVAALRDAIDAKAKEWRDIVKIGRTHMQDATPITLGQEWSGYAGMLSDDLERIEDS
                     LNGVYRLSLGGTAVGTGINAAPGFAEAAAAEIAKLTGLPFVTAPNKFAVQGAHDALVQ
                     LSGAFRTLAVSLYKIANDIRLMACGPRAGFAELILPENEPGSSIMPGKVNPTQAEALT
                     MIAVQVMANDVAVGFAGASGYLEMNVYKPLMIYNIAQSIVLLSDGCANFRKFLIEGTK
                     PNLKKIKDYVDRSLMLVTALAPVIGYDKASQIAHHAMEHDLTLKEAALALGFVSEHEF
                     DRIVDPAKMVRPYVAK"
     gene            complement(9795..12170)
                     /locus_tag="VKU03_04005"
     CDS             complement(9795..12170)
                     /locus_tag="VKU03_04005"
                     /inference="COORDINATES: protein motif:HMM:NF044972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3772 domain-containing protein"
                     /protein_id="HLJ70456.1"
                     /translation="MTAPPRLSVLLFAFLALFLAFAGQTRAQTPESVRSQIESVRATL
                     LEIDTAFKNAPLSDVELLRLRAECDPLAGVLQGVIDDLTPKLAASAKRLAELTPKSKE
                     KTPGATDGAAADIAAEQKIHDDLDASMRTARALAVQVDETSARIGAARRALFAAQTFA
                     RSSSILSPLLWATVIRETPRDVAALGGLLKAWASDDIARASAGQWLGFIAIILALVAL
                     AAPLRWVTLRVIARDPKLAEPGRLRRALAALWTALVLACLPILALLAVSYALDAFDLS
                     YPQLQGVQEAFFDGLKFLFVANAIAHGLLAPGQPQWRVIDLSEPVVRRCLGIWLGAAA
                     IVALEKVLEPVDDAVSASLSVTVATRGACALVAALLMAQALRRIYAPREAAPLGQAPR
                     DAWGSARALAWLLTLAIFVSVIVGYVAFATFLLGQIVRDVGAIAILYLVSVVVDAGAE
                     VALQPKAMIGRSLMMMAALRKESLEQLAVLIQGVARVTFFVAAALLILGPWGIQSQDM
                     LGSLRAAYFGFKYGDVTISVSSIVAATFVFILGVLITRTVQNWLASQLLPRTRLDDGL
                     RNSVKTIFGYIGFVLALALGAGQIGLDFQKLALIAGALSVGIGFGLQSVVNNFVSGLI
                     ILWERAVRVGDMVVVGPDSGFVRRINVRSTEIETFDRAMLIVPNSNLVSGVVKNWVRA
                     DRVGRIIIAINVAFDSDPEAVREILIAAAKAQDLVLSIPAPLVLFNEFGEWAMKFQLI
                     CFVDEIEMADRVKSEILFDLHRRLKAANLRVPYPRQDFDWVGEERGEKKLA"
     gene            12345..13475
                     /locus_tag="VKU03_04010"
     CDS             12345..13475
                     /locus_tag="VKU03_04010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453441.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase regulatory
                     subunit"
                     /protein_id="HLJ70457.1"
                     /translation="MDPTSTLLAADAAGARALERLIAAGFDRIEPPALQPASVFLDMS
                     GEEIRGRLFLTSGPAGEELCLRPDYTIPVCLAYLASPEAGRSAQYSYLGPVFRARAGQ
                     AGEWRQSGLESYGRDDAEAAEAEIFALAMEAAAAAGAGALDVRFGDAGLFDRALRALD
                     WPDALHRRLRRGLAQGRSLDSILDETANGSAAQAGVLAALERTDHAGAKALVEDLLAI
                     AGIAAVGGRSAGEIADRFLEQASLRAGSSAPAEKREALRRFLAIKGDPDSASRQLRAL
                     AADAKLDLAEALDSFDQRTGFLAARGVAVERCTFAAGFVRDLDYYTGFVFEAVDPQRA
                     DARPIIGGGRYDRLARRLGAARDIPAIGAAIWIDRIAEGKSR"
     gene            13472..14467
                     /gene="hisG"
                     /locus_tag="VKU03_04015"
     CDS             13472..14467
                     /gene="hisG"
                     /locus_tag="VKU03_04015"
                     /EC_number="2.4.2.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003879 - ATP phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HLJ70458.1"
                     /translation="MNGADETPLVLAVPSKGRLQENAAAFFARAGLDFVQGRGARDYR
                     GAIAGLAGVEVAFFSASEIVGQLASGAVHLGVTGEDLVREQVADADQRLALLAPLGFG
                     KANVVVAAPQAWIDVRTMADLEDVAASFHARRGERMRVATKYVNLTRRFFAEKGVGDY
                     RIVESLGATEGAPAAGAADLIVDITTTGATLAANALKALDDGIILRSQANLVASLRAP
                     WGEKQREIARAMLMRIAAAQQALACRELRARLPGGAAGVFEAAAAMFDARPAFGEAAD
                     ASGRITLHAPKQKVAELAAWLLAQGAEHVSVSALEHVFSARNVLYEALEKRIGAP"
     gene            14598..15497
                     /locus_tag="VKU03_04020"
     CDS             14598..15497
                     /locus_tag="VKU03_04020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004447264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase II"
                     /protein_id="HLJ70459.1"
                     /translation="MPRNADDLVALLDLEKIEEDIFRGLSPANGWKRTFGGQVIAQAL
                     MAATRTVDPSRAAHSLHGYFILPGDPEAPIIYEVDRIRDGGSFTTRRVVAVQHGKAIF
                     SLSASFHVAESGFEHFIPAPDVPDPERLIDAGALMRAAGLKPEQVERFIDRISPVEFR
                     PVDPSRHAPLKSGEKREPSQALWIRINSPLPDDPIIHRAALAFLSDMTLLGTSVVAHG
                     RNLGEPEIQAASLDHALWLHRPFRADEWLLYAQDSPSAGGARGFNRGLLYSRDRTLVA
                     SVAQEGLIRKRTGDKARRHLPSS"
     gene            15728..16066
                     /locus_tag="VKU03_04025"
     CDS             15728..16066
                     /locus_tag="VKU03_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007559495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HLJ70460.1"
                     /translation="MKIVMAIIKPFKLEEVRDALTAIGVHGLTVTEVKGYGRQKGHTE
                     IYRGAEYAVSFLPKLKIEVAVAAELVDQVIEAITAAARTGQIGDGKIFVTALESAVRI
                     RTGEKDADAL"
     gene            16108..>16790
                     /locus_tag="VKU03_04030"
     CDS             16108..>16790
                     /locus_tag="VKU03_04030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70461.1"
                     /translation="MRLSRFFIRLAPALAIAAFIGFAAPSPSSAEDQPAAPAAAAPAA
                     SAPAAATPAPAATAAAPAAQAGPPACAAAGPNNSPPAVVTKCTPNSGDTAWMLTSMAL
                     VLMMTVPGLGLFYGGMVRKKNVGDTVMTSFAIACLVTVIYAVLTYSLAFTGGSAFIGG
                     LSRVFLQGIVSDIANGVGNPNPLAPTIPETVYMCFQMTFAIITPALIAGAFAERMKFS
                     AMLWFVGLWS"
BASE COUNT         2821 a         5713 c         5445 g         2811 t
ORIGIN      
        1 gaattttcga ctcaggttgt tttccgaatt cggccttgag cagtcgatcg tcgacacgat
       61 actggccggc ggccgcttgc tggacttgcc cgaataggct cggcggccag tccttggcga
      121 tcgcgcctga gacggagggg ctgacatggc gcgcgtcctg tttctcgtct tttccggcgg
      181 cggcaatctt ccgccgagcc tcggcatcgc ccgggcctta agggaacgcg gccacgaggc
      241 gattttcgcc ggcgagccgg agatgatccc gcgcgtgaag ccgacgccgt ttcgcgccat
      301 cgaattcgcc gacgcctatg cgcaggtcga cctctacaag aaatctccat tcggcgccgt
      361 cgtttgctac ctttcctcgc cggccgtcgc ggcggaggct cgcgcgatca tcgaaacgga
      421 aaagcccgac ttcattctcg tcgacttctt gttcttcgcc gcgcgcctcg ccgcaaaagc
      481 ttgcgaacga gccaacgcga tcatggcgca tatggcgctg ctgcgcgtcg ccgaaccgtt
      541 tcgcggcctg atcgaccatc tcaacggcct gcacgacgag gcgggcttgg cgccgctgcc
      601 gtccttcgac acgctcacat tttcgggcga gcccgtcatc gtcacgtcgc tcgccgccgt
      661 cgagcaaggc cgagcggcgc ttaagggcgc cggcaatgtc cgctatgtcg ggccggcatt
      721 ggagatggag gcccatgcgc ggccgctcgc ccttccctgg gatagagacg atccgcggcc
      781 gctcgcgctg atcagcttct cgaccgatcc gattcaagcc gatcccgatc tgatccagcg
      841 cgcgctcgac gcgctcgccc cgctcgatct gcgcgtcgtg gcgacggtcg ggcgcgcggt
      901 cgatcgcggc cgcctcgccg tcccgccgaa cgccctcgcc gttgaagcgg ccgatcatga
      961 tcggctgatc gcggcggcgg ctttggtcgt cacccatggc ggccatggca cggtgatgcg
     1021 ggcgctgaag cacggcgcgc ccatgctcgt cctgccgggc ttcattcccg accaggcgcc
     1081 caacgccacg ctggtcgcgg acttgggcgc tggccgcatc ctcggccagg attccgctgt
     1141 ggcggccatt cgggcggccg cggaggccat cctgagaaat cccgattacc gcgccaaagc
     1201 gcgccgcgct gccgggctgt tcggccggcg cgacggcgcc gttggcgccg ccgacgaggt
     1261 cgaaaagctc ctcgcccgaa aggccgccgc caagcgggcg tgaaccgcaa agtgtcgtcc
     1321 agctctttgc cgacgccgat ttcgtcccta tatctgctcg ccgccgcttc attcgcggcg
     1381 cgctcatgct gggggtctct ccttgcccgt cattctctcg cgcctgcgcg cgctcacggt
     1441 cctgctcgcc atcggcctga cgctctcggc ctgcggctac aatcgcatcc cgaccaggga
     1501 agaagccgcc aaggcggcct gggcggaggt gcagaatcag tatcagcgcc gctccgacct
     1561 cattcccaat ctcgtcgcaa ccgtgcaagg ctacgccaag caggaaaagg acgtgctgac
     1621 ccaggtcacg gaagcgcggg ccaaggcgac cgcgatccat gtcgacgcct cgcaactgac
     1681 cgatcccgcg aaggtcaagc aattgcagga cgcgcagaac gcgctctccg gcgcgctcgg
     1741 cagactgctg gcggtcagcg agaactatcc cgagctgaaa tcgaaccaga atttcctcgc
     1801 gctccaggcg cagctcgaag gcaccgagaa ccgcatcgcg gtcgcgcgca gggactatat
     1861 cgacgcagtg cgcgcctata atctcgagtt gcgcaccttc cccggcgtcc tctgggcctc
     1921 gaccttcttc cgctccaacc agccgatggc cgagttcgca gccgatagcg gcgcgcaagg
     1981 cgcgccgaaa gtgaagttct agagcagcga aacccttgga gcgcctctcc cgtcattgcg
     2041 agcgaagcga agcaatccag gaacctcggc ggctcctgga ttgcttcgtc gcttcgctcc
     2101 tcgcaatgac gacgttggct cttgcatttt tcctctgcgt cccggccatc gcagccgagc
     2161 tcaatttccc gccgctcagc ggccgcgtcg tcgaccaggc caatatcctc tctcccgata
     2221 cgcgcggggc gctggcttcg aagctaaaag acttggagga caaatccggc attcagctcg
     2281 ttgtcgcgac cgtctcatcg ctgcaaggcc aggagatcga gccttacgcc aatcagctct
     2341 tccgcacttg gcggctcggc gaagcaaaga aaaacaacgg cgtcctcttc cttgtcgcgc
     2401 cgaaggagca tcgcgtccgc atcgaggtcg gctatgggct cgaagggacc ctcaccgacg
     2461 cgacgtcgaa aatcatcatc gcgaacgccg tcgcgccgcg cttcaaggcc ggcgatttca
     2521 acggcggcgt tcagcgcggg gtcgatgaca tcgtcaccgc gctgaccgcc gattccgccg
     2581 aatggcagaa gcggccggcg gtgcgggagg aggacaattc gagcctcgtc gcgctcttgc
     2641 ccctgctcct tttcgccgcg atctttctgc tggtctttat ccatacggtg cgaagccgcg
     2701 gcgggatcgg cccagccttg ctctacatgc tgatgagcgg cggcaattcg cggcgcggcg
     2761 atttcggcgg cggcggcttt ctgggcggcg gcgacagctt ttccggcggc ggcggctcgt
     2821 cgggcggcgg cggcgcttcg gggagctggt gatgacggtc agccatgacg acgcggcccg
     2881 catcgaagcc gcgatccgcg ccgctcaagc gcggtcgagc ggtcagatcg ttttcgtcct
     2941 ggcccgcgcc tcggcgaatt acgaggtcat gccgttcgtg tggagcgccg tgctcgcgtt
     3001 gctgacgccc tggccgctgc tcgacctcac cgaaatttcg gccgagcgca tttacctcgc
     3061 gcagctcgcg gtcttcatcg ccgcgctcct cgtgctgacg ctgccgcgcc tgcgccttct
     3121 gctgacgccg gcgcgaatca ggcgcgtcaa tgcgcatcgc gcagcgctcg aacaattcgc
     3181 cttgcgcggc ctcgcccata acgaggcgcg caacggcgtg ctgctctatg tttcgctcgc
     3241 cgaacggttc gcccgcatca tcgccgacga cgccgcggcc aaagcggttc cgcaaaaaga
     3301 gtggcaggct ctgatcgatc ggctgctcac cgagatgaaa agcggtcaaa ccgccgaggc
     3361 gctgatcggc gccgcagcgc ggtgcggcga tttgctggcg caaagcttcc cgccgcgcga
     3421 cggcgccgcg cgcccgtcct ttcaccgatt tcacgtcatc tgaagcaatc gttcataggg
     3481 cggcgcccct atacgagcgg attgcttcgc tttgtccgca atgaccggat tcgcaatgac
     3541 cgcgctaaag cgacggcggc tgtggcccct tggcggcggc gagaatggcc gagggcgcgc
     3601 tgcggccgcc ttcctgaacg atgacggcgt aggaatcggc gccgttgagg cgagcctgat
     3661 cgaggctggc tttcagcaca atcggcgcgc ctgtccattc gccgactttg gtgatcgcgc
     3721 gcgcgacgtt ggaataagtc agcgtcgagc cggcattctc gccgcgcttg atggcgacag
     3781 tacgtttgct cgccaaggcg atcagataaa caccggcttt atggctcttc cccgaaggcg
     3841 ccgcgccgat ggagatggcg atcgagccgt cctgctcgga gaccgatagc ggcgtcgaca
     3901 agacgtccgg ccgtttgcga gtctgcgcct ccgccgtctc gatctgcgcc cggtcgctgc
     3961 cgaccgcgtc ggcgacgccg ttgatgatcg cctgcggggt gtagacttcg ccgcggccgc
     4021 tcgccctggc gtaggcgcgc tggcgcgccg tgtagaccgg ctcggcgagc gtatctttcc
     4081 agccgatata gtcccagtag tcgaccggca gggagatggc gagcgtttcg ggatcgcgag
     4141 ccaattccgc aaggatgcgg tcggccggcg ggcatgacga acagccttgg ctggtgaaga
     4201 gttcgaccac ggctttcacc ggcgaggcgt ccgccgcaag cccgagcgtc gaatagatga
     4261 ccatgccggt cgcggccagc gccgcaaccg ccgcgcgctg gactgacata aaactcatcg
     4321 tccgaaatcc atgatgagcg ccttgataat aggcgattgc gcccctaagc caatcaattc
     4381 tgggtgactc ttggccacga accgcgcccc gagcgctttt ggcgcttaga ggccccgccc
     4441 tggattcgcc atggacggcc gcgaagcttt cgattaatga gggttgaacg gcttcgatcc
     4501 gcttcgccgc gccgcagcgc gagccggaag agcgcgcagt ttttacgaaa agagacgcat
     4561 tgatgcagtt gggccgctgg cgcgccggaa tcctcgcaac gctgctcgcg cttgtcgccg
     4621 gcccggcctt cgccaccccc gctttggtga tcgacgctga tagcggcaaa gtcctttacg
     4681 agcgccaggc gaccgccaat tggtaccctg cctccctgac caaattgatg acgacctatg
     4741 tcgcgctgag cgcggtgcgc gagggacgcg tcacgctcga tacgcccttc gtcatgtcgc
     4801 agcgcgcagc gagcatggcg ccgtcgaaga tgggctttaa gccgggcacc gaggtgacgc
     4861 tcgacaatgc gctgaagatg ctgatggtca aatcgccgaa cgacatcgcg gtgatgatcg
     4921 ccgagggcgt ctccggctcg gtcgagagtt tcgccgacga gatgaacgcc gccgcggcca
     4981 agctcggcat gaaggaaacg catttcgtca atccgaacgg attgcccgac gaccggcaag
     5041 tgacatcggc gcgcgacatg gcgatcgtcg cccgcgcctt gctgaaggag tttccggacg
     5101 aagccggcat tttcggcatc ggcgcgctgc agctcggcaa ccggatcatt ccgacgcata
     5161 atggcttgat cggccgctac ccgggcgctg acggcatgaa aaccggcttc acctgcgccg
     5221 ccggcttcaa ccttgtcgcc agcgccacgc atggcggcaa gcgactgatc gccgtgattt
     5281 tgggcgcggc gacgccgcgc gagcgcaccg cgatggcgat tgcgctgttc gagcgcggct
     5341 tcgcggaaac cggcggtctc gccggcttgt tcagcggcgg cggatcgggc ctcggcctcg
     5401 tcgacgccct cccctcgccg ggcgtcggcc cagccccgga catgcgcgcg gaaatatgct
     5461 cgcggcgcgg ccgcgccgcc ctgctcgccg ccgaggagga ggacatgcgg gccgccgcgc
     5521 cgcccgccgt ggccgccgcc cggaccggcg caacgcgcgg catgagcctt ttcgccgccg
     5581 cggcggcctc gaccgacccc gaggcgatgg ccgcggcgcc ggcgccgcat ttcgatccgg
     5641 tgcgggtctt cgtcggcccg aagccaggct ggacaggccc ggtcgccatg gccaagccgc
     5701 agcctttcga agcgccggaa agcgccagcg cctatgccgc cgggccggac aaggaagcgg
     5761 gcgattcgaa tcaggcgacc agcgcgccga tggcgctgca gggcgcggtc aagccgacga
     5821 agctcggctc cttggtcgcg ccgcacaaac ctgaggcgaa aagggcgcac gcgatgaagc
     5881 aggggaagaa aatcgctacc gtcctgacgc cgaaaaaacc agctttttca aaggttccga
     5941 tagggaaacg gacgcgctcg acggcccatt gaccgcggcc ttattatcat cacccagccg
     6001 tgatcgcggc catattttcg cccggatacg gtcaattgcg cgccgcaacg ctcaggcatt
     6061 ctgagtgtct agccgcgaag gagcgatttg gccgctgctc ggtttttcag ccccccagcc
     6121 cccgagcgcc agtcaggtcg atagtttgcc tcgcaggcca gcggaccggc ggaacccctc
     6181 cagccggtcc gctttttttt gcccttttca aagtctgcgc tgctccggcc gcttgcaagc
     6241 cgcgccgccg gccgcgatat gaagcgccgt ccggctcctc cgagacggat ttaagcaagg
     6301 acaattcaat cattaagacg aggctgttgt gacgaccgac gttcatccga atgccgacga
     6361 ggccaaaacg caaagcttcg aggcggatgt ttcgcgcttg ctccatttga tggttcattc
     6421 ggtctattcg gaccgcgaca ttttcgtgcg cgagctcgtc tcgaacgccg ccgacgcgtg
     6481 cgagaagctg cgttacgagg ctctggccaa gccggagttg atcgaaggcg gctcgccgtt
     6541 cgcgatcacg atcgccatcg atcccgaggc gaagacgctg accttctccg ataacggcgt
     6601 cggcatgagc cgcgccgacc tcgccgaggg cctcggcacg atcgccagat ccggcacccg
     6661 cgcctttctc gacaagctca gcgcggcgga atcggagaag gaaggctcaa acctgatcgg
     6721 ccaattcggc atcggcttct attcggcctt catggtcgcg gacgaggtcg tggtcgagac
     6781 gcggcgggcc ggttcaagcg aagcctggcg ctggtcgtcc gacggcaagg gcgcctatac
     6841 gatcgcgccg ctgccgctgg atgaggcgcc gcagcgcggc tcgcgggtga tcctccgcct
     6901 caacggcgcc tcgcatgact acgccgagcc gcatcgcctc gaacggatcg tgcgcgagca
     6961 ttccggcgcc gtcgccgtgc cgatcgacct gatcgagaag cccggcgccg agccgcgccg
     7021 catcgccgac ggcgcggcgc tctgggccaa gccgaaatcc gaggtcaagc cggaagaata
     7081 cgcggaattc tatcgcgggc tcgccgggca attcgacgag ccggccctga ccatccacta
     7141 ccgcgcggaa ggccgccacg aatacacggt ccttgccttc atcccgggct cgcggccttt
     7201 cgatcttttc gatccggtgc gcaagggccg caacaagctc tatgtgcggc gcgtgctgat
     7261 cagccaggat acggacatcc tgcccggctg gctgcgcttc gtccgcctcg tcgtcgacag
     7321 cgccgacctg cccctgaatg tctcgcgcga gatgatccag gagagcccgg ttttcatggc
     7381 gatcaagaag ggggtgacca atcgcatcgt ccaggagctg agcaagctcg ccgaaagcca
     7441 agaggaatcc gacaaagaga agttcgccaa aatctgggag aatttcggcg cggtgatcaa
     7501 ggaaggtctc tacgaagacc cggagcggcg cgacggcttg ttcaagatcg cgcgcttcgc
     7561 cacatccgcc tctcccaagg gcgatcgcac gctcgccgac tatgtcaaga atttaaggga
     7621 aaatcagact gcgattttct atctgaccgg cgaggacttg aagcgcatcg agaccagccc
     7681 gcagcttgag ggattccgcg cccgcggcgt cgaggtcctc ctgctcgccg acccggtcga
     7741 cgcgttctgg gtgtcgaccg cggcggggta cgacggcaag ccgttcaaat cggtcacgca
     7801 aggcgccgcc gagatcaaga ccattccgct cgtcgaaggc gccaaatctg ggggcgaggc
     7861 gccgtcggcg gaggtcgcga cgctcttcgc cttcttcaag cagacgctgg gcgaggcgat
     7921 cggcgaggtg cgcgcctccg agcgcctgac cgacagcctc gcctgcctgg tcgcgccgga
     7981 attcggaccc gaccggcagc tcgaaaagat gctcgccgcg catggccggc tgagcgatcg
     8041 cgccaagccg atcctcgagg tcaacgccgg ccacccgctc gtcgcgtccc tcgccaagcg
     8101 ctatcgcgaa gccgccgaca aatcgctggt cgaggatgcc gcttggctgg tcttcgacga
     8161 ggcgcggctg atggagggcg aaacgccggt cgacgcgcag gccttcgccg gaagattgcg
     8221 ccgcgtgatg gagaaggcgc tggggtagga gccgaccgca acggcccctt ctcctcgctg
     8281 tgcggcgcga ggggcctact tcgccacgta aggccgcacc atcttggcgg gatcgacgat
     8341 gcggtcgaac tcatgctcgc tgacgaagcc gagcgccagc gcggcttcct tcagcgtcaa
     8401 atcatgctcc atcgcgtgat gggcgatttg cgaagccttg tcgtagccga tcaccggcgc
     8461 gagcgcggtc acgagcatca atgagcgatc gacatagtcc ttgatctttt tcaggttcgg
     8521 cttcgtcccc tcgatcagga acttgcggaa attggcgcag ccgtcgctca gcagcacgat
     8581 cgattgcgcg atgttgtaga tcatcagcgg cttatagacg ttcatctcca gatagccgct
     8641 cgcgccggcg aatccgacgg ccacgtcatt ggccataacc tgcacggcga tcatggtcag
     8701 cgcctcggct tgggtcggat tgaccttgcc cggcatgatc gacgagccgg gctcgttctc
     8761 cggcaggatc agttccgcga agccggcgcg cggtccgcac gccatcaggc ggatgtcgtt
     8821 ggcgatcttg tagagagaga cggcgagagt gcgaaaggcg ccggaaagct ggaccaacgc
     8881 gtcatgcgcg ccctgcacgg cgaatttgtt cggcgcggtc acgaaaggca ggccggtcag
     8941 cttggcgatt tccgccgccg cggcttcggc gaagccgggc gcggcgttga tcccggtgcc
     9001 gaccgcggtg ccgccgagcg acaggcgata aacgccgttc agcgaatcct cgatccgttc
     9061 gagatcgtcc gacagcatgc cggcatagcc cgaccattcc tggccgagcg tgatcggcgt
     9121 cgcatcctgc atatgggtgc ggccgatctt gacgatgtcg cgccactctt tcgctttggc
     9181 gtcgatcgcg tcgcgcaagg cggcgacgga gggaagcaag cgccgccgcg tattcaccgc
     9241 cgcggcgata tacatggccg acggaaaatt gtcgttggac gattgcgaca tgttgacgtg
     9301 atcgttcgga tggaccgggc tcttgccgcc gagcggcgtt ccggcgagtt ggcagcaccg
     9361 gttggagatc acctcgttga cgttcatgtt gaattgcgtg ccgctgccgg tcatccagac
     9421 atgcagaggg aacatgtcct cgtgccggcc ttcgagcagc tcgtcgcaga ccgtcacgat
     9481 cagatcccgc ctctccttgt cgagccgtcc gccggcatga ttggcctcgc tcgccgcctt
     9541 cttcaggatc gcataggcgg cgatcatctc gcgcggcatg agatcgcgcc cgatgctgaa
     9601 atgctccagc gagcgctgcg tctgcgctcc ccaaagcttg tccgccggaa catccacttt
     9661 gccgaggctg tcggtttcgc tgcgtacggc gcccattgtc ggctccaaag cttggaaaga
     9721 cgcggtcttt cgattcatag gccgagcggc cggcgtttcg ccaataaaca gttcggcatt
     9781 gtccgccgaa cggcttaggc cagcttcttc tcgccgcgct cctcgccgac ccaatcgaaa
     9841 tcctggcgcg gatacggcac gcgcagattg gccgccttga ggcgacggtg cagatcgaaa
     9901 aggatttcgc tcttcacccg gtccgccatc tcgatctcgt cgacgaagca gatgagctgg
     9961 aacttcatcg cccattcgcc gaattcgttg aacaggacca gcggcgccgg gatcgagaga
    10021 accagatctt gcgccttggc cgcggcgatg aggatttccc gcaccgcctc cggatcggag
    10081 tcgaaggcga cattaatggc gatgatgatc cggccgacgc ggtcggcgcg cacccagttc
    10141 ttgaccacgc cggagacgag gttggaattg ggcacgatca gcatcgcgcg gtcgaacgtc
    10201 tcgatttcgg tcgagcgtac gttgatgcgc cggacgaagc ccgaatccgg gccgacgacg
    10261 accatgtcgc cgaccctgac cgcgcgctcc cagaggatga tcaggccgga gacgaaattg
    10321 ttgacgaccg attgcaggcc gaagccgatg ccgaccgaca gcgcgccggc gatcaaggcg
    10381 agcttctgga aatcgaggcc gatctggccg gcgccgagcg ccagggccag gacgaagccg
    10441 atatagccga aaatcgtctt gaccgaattg cgcaagccgt catcgagccg ggtgcgcggc
    10501 agcaattgcg aggcgagcca gttctgcacc gtccgggtga tcaagacgcc gaggatgaag
    10561 acgaaggtcg cggcgacgat cgacgagacg gaaatcgtca cgtcgccata tttgaagcca
    10621 aaataagcgg cgcgcagcga gccgagcatg tcctgcgatt ggatgcccca gggcccgagg
    10681 atcagcagcg cggcggcgac gaagaacgtg acgcgcgcga cgccctggat gagcaccgcc
    10741 agttgctcca gggattcctt gcgcaatgcc gccatcatca tcaggctgcg cccgatcatc
    10801 gccttgggct gcagcgcgac ctccgcgccg gcgtcgacga ccacggagac gaggtagaga
    10861 atcgcgatcg cgccgacatc gcggacgatc tggccgagca ggaacgtcgc gaacgcgaca
    10921 tagccgacaa tgaccgagac gaagatggcg agggtcagca gccaggccag agcgcgcgcc
    10981 gacccccagg cgtcgcgcgg cgcctggccg agcggcgcag cctcgcgcgg cgcatagatc
    11041 cggcgcagcg cctgcgccat cagcaaagcc gcaaccagcg cgcaggcgcc gcgcgtcgcc
    11101 accgtgacgc tgagcgatgc ggagaccgcg tcatcgaccg gctcgagcac tttctccaag
    11161 gcgacgatcg ccgccgcgcc gagccaaatg cccaggcagc gccgcacaac cggctcggaa
    11221 aggtcgatga cgcgccattg cggctggccc ggcgccagca ggccatgggc gatggcgttg
    11281 gcgacgaaca ggaatttcag cccgtcgaaa aacgcctcct gaacgccctg caattgcgga
    11341 taggagagat cgaacgcatc gagcgcgtag gagacggcca acagcgcgag gatcggcagg
    11401 caggccagaa ccagcgccgt ccacaaggcg gcgagggcgc ggcgcagccg gcccggctcg
    11461 gccagtttgg gatcgcgcgc gatcaccctc agcgtcaccc agcgcagcgg cgccgccaag
    11521 gcgacgaggg cgagaatgat ggcgatgaag ccgagccatt gcccggcgct ggcgcgggcg
    11581 atgtcgtcgc tcgcccacgc tttgagcaag ccgcccagcg cggcgacgtc gcgcggcgtc
    11641 tcgcgaatga cggtggccca gagcagcggg ctcaaaatgc tcgacgagcg ggcgaaggtc
    11701 tgcgcggcga agagcgcgcg gcgcgccgcg ccgatccgcg cgctggtctc gtcgacctgc
    11761 accgcgaggg cgcgggccgt gcgcatcgaa gcgtcgagat cgtcgtggat tttctgctcg
    11821 gcggcgatat cggcggcggc gccgtcggtg gcgcccgggg ttttctcttt cgatttcggc
    11881 gtcagctcgg cgagccgctt ggcggaagcg gcgagcttcg gcgtcaaatc gtcgataacg
    11941 ccctgcagca cgccggcgag cgggtcgcac tcggcgcgca agcgcaggag ctcgacatca
    12001 gagagaggcg cgttcttgaa cgccgtatcg atctcgagca gcgtcgcgcg aaccgattcg
    12061 atctgcgagc gcacggattc cggcgtctgc gctctcgtct ggccggcgaa agcgagaaac
    12121 aaggccaaaa acgcgaaaag gagaacgctg aggcgcggcg gggcggtcat tttccttaag
    12181 aatcgccgaa atggcggctc cttccagcat tcgaccatgg cggccctggg gcggcaagcg
    12241 aagcccctgt ggacggacca ttcgttcacc ctggcgcggg aaagaggctg cgcccggcgg
    12301 cccgctttgt taaaggaagc gccaaattcg aaagatctga tcccttggac ccgacttcga
    12361 ctctcctcgc cgccgacgcg gccggcgccc gcgcgctcga aaggctgatc gcggcgggct
    12421 tcgatcgcat cgagccgccg gctttgcagc cggcctccgt gtttctcgat atgtcaggcg
    12481 aggaaatccg cggccgcctg ttcctgacca gcggcccggc cggagaggag ctgtgccttc
    12541 ggcccgatta cacgatcccg gtctgcctgg cctatctcgc ctcgccggaa gccggccggt
    12601 cggcgcaata ttcctatctc ggtccggtct ttcgcgcccg agccggccaa gcgggcgaat
    12661 ggcggcaaag cggccttgaa agctacggcc gcgacgacgc cgaggccgcc gaggccgaga
    12721 ttttcgcgct cgccatggag gcggcggccg ccgccggcgc cggcgcgctc gacgtgcgct
    12781 ttggcgacgc cgggctgttc gaccgcgcct tgcgcgccct cgactggccc gacgccctgc
    12841 accggcgctt gcgtcgcggc ctcgcccagg ggcgctcgct cgattccatt ctcgacgaga
    12901 cggcgaacgg ctcggccgcg caagccggcg tgcttgccgc tttggagcgg accgaccatg
    12961 ccggcgccaa ggccttggtc gaggatttgc tggcgatcgc cggcatcgcg gcagtcggcg
    13021 gccgcagcgc cggcgagatc gccgaccgct tcctggagca ggcgtcgctc cgcgccggct
    13081 ccagcgcgcc ggccgagaag cgcgaggcct tgcgccgttt tctcgccatc aagggcgatc
    13141 ccgattcggc gtcgcggcaa ttgcgcgctc tcgccgccga cgccaagctc gatctggcgg
    13201 aagcgctcga ttccttcgac cagcgcacgg gctttctcgc cgcgcgcggc gtggcggtcg
    13261 agcgttgtac tttcgccgcc ggcttcgtgc gcgatctcga ctactatacc ggcttcgttt
    13321 tcgaagccgt cgatccccaa cgcgccgatg cgcggccgat catcggcggc ggccgctacg
    13381 accgtttggc gcgccgcctg ggcgcggcgc gcgacattcc cgccatcggc gccgccattt
    13441 ggatcgaccg catcgcggaa ggaaaaagcc gatgaacggc gccgacgaaa cgccgctggt
    13501 cctcgccgtc ccgtcgaaag ggcgcctgca ggaaaacgcc gccgccttct tcgcccgggc
    13561 cgggctcgac ttcgtgcaag ggcgcggcgc gcgcgactat cgcggcgcga tcgccgggct
    13621 tgccggcgtc gaggtcgcgt ttttttccgc ctccgaaatc gtcgggcaat tggcgagcgg
    13681 cgcggtccat ctcggcgtca ccggcgagga tctggtgcgc gagcaggtcg cggacgcgga
    13741 ccagcgcctg gcgcttctcg cgccgctcgg tttcggcaaa gccaatgtcg tcgtcgccgc
    13801 gccgcaagcc tggatcgacg ttcgcaccat ggccgacctt gaggacgtgg cggcgagctt
    13861 ccacgcgcgt cgcggcgagc gcatgcgcgt cgcgaccaaa tatgtgaacc tcacccgccg
    13921 cttcttcgcc gagaagggcg tcggcgatta tcgcatcgtc gaaagcctcg gcgcgaccga
    13981 aggcgcgccg gccgccggcg ccgccgacct gatcgtcgac atcacgacga ccggcgccac
    14041 cctcgccgcc aatgcgctga aggcgctcga cgacgggatc attctgcgct cgcaggccaa
    14101 tctcgtcgcg tcattgcgcg cgccctgggg cgaaaaacaa cgcgagatcg cccgcgccat
    14161 gctgatgcgc atcgccgccg cgcagcaggc gctcgcctgc cgcgaattgc gcgcacgcct
    14221 gcccggcggc gccgcaggcg ttttcgaggc cgccgcggcg atgttcgacg cccggccggc
    14281 cttcggcgaa gcggccgacg cgtcgggccg gatcacgctg cacgcgccca agcagaaagt
    14341 cgccgagctc gccgcctggc tgctcgcgca aggcgccgag catgtcagcg tcagcgcgct
    14401 ggagcatgtc ttcagcgcga gaaacgtact gtacgaggcg ctggagaagc gcatcggggc
    14461 gccgtaaacc cttctcccat cgacctcccg ggcggtgacg atcggtcgtt cgccatagcc
    14521 gcgcgaggtt gcaacggccc gccgcaacgc gcatgagaga agacgaccaa gcgcgaacgt
    14581 cagcttcgag gaagcccatg ccccgcaatg cggacgatct tgtcgccctg cttgatctcg
    14641 aaaagatcga ggaggacatt ttccgcggcc tgtcgccggc caatggctgg aagcgcacgt
    14701 tcggcggcca ggtcatcgcc caggcgctga tggcggcgac gcgcacggtc gatccctcgc
    14761 gcgccgccca ctcgctgcat ggctatttca tcctgcccgg cgatccggag gcgccgatca
    14821 tctacgaggt cgaccgcatc cgcgacggcg gcagcttcac caccaggcgc gtcgtcgccg
    14881 tccagcacgg caaggcgatt ttctcgctct cggcctcgtt ccatgtcgcc gagagcgggt
    14941 tcgagcattt catccccgcg ccggacgttc ccgatcccga gcggctgatc gacgccggcg
    15001 ccttgatgcg ggccgccggg ctcaagcccg agcaggtcga gcgcttcatc gatcggatca
    15061 gcccggtcga atttcgtccg gtcgatccct cccgtcacgc gccgctcaaa agcggcgaaa
    15121 agcgcgagcc gagccaggcc ctgtggatca ggatcaacag cccgctcccg gacgacccga
    15181 tcatccaccg cgccgcgctc gcctttctct cggacatgac tttgctcggc acctccgtcg
    15241 tcgcccatgg ccgcaatctc ggcgagccgg aaatccaggc cgccagcctc gaccacgcgc
    15301 tttggctgca tcgtcccttc cgcgccgacg agtggctcct ctacgcccag gacagcccca
    15361 gcgcgggcgg cgcgcgcggc ttcaatcgcg gcctcctcta ttcgcgcgac cgcacgctgg
    15421 tggcgagcgt cgcgcaggaa gggctgatcc gaaagcggac cggcgacaaa gcgaggcgtc
    15481 acttgccttc atcttaggcg cctgtttttt gggcgttcgc gcggcggatt tgcgattggc
    15541 gttggactcg cttccgccgc ctccaaaatc cgcggccttt tcataggctt gccggcatcg
    15601 gccggcttgg cacagcgctt gttaaggctc gcccgagcgg ggcgggcctt gtcagcgcgc
    15661 ccagccgcca ccgtgcggag cgttgcgctt tgggggcgcg ccgcttcgcc aagctgggat
    15721 gaggcacatg aaaatagtga tggcgatcat caagccgttc aagctcgaag aggtgcgcga
    15781 cgctttgacc gcgatcggcg tgcacgggct gaccgtgacc gaggtcaagg gctatggccg
    15841 tcagaagggc cataccgaga tctaccgcgg cgccgaatac gccgtgagtt tcctgcccaa
    15901 gctcaagatc gaagtcgccg tcgccgccga gctggtcgat caggtgatcg aggcgatcac
    15961 cgcggcggcc cgcaccggcc agatcggcga cggcaagatt ttcgtcaccg cgctcgaatc
    16021 ggcggtgcgc atccgcaccg gcgaaaaaga cgccgacgcg ctgtgaccca ttcgtccttt
    16081 cgactcgcaa ttcggagctt caacgcaatg agactctcca gatttttcat acgcctcgcg
    16141 ccggctttgg cgatcgccgc gttcatcggc ttcgccgcgc cgagtccctc cagcgccgag
    16201 gatcaaccgg ctgcgccggc cgccgctgcg cctgcggcgt cggcccccgc ggccgcaacg
    16261 cccgcgcccg cggcgacagc cgccgccccc gccgcgcagg ccggcccgcc ggcttgcgcc
    16321 gccgccggcc ccaacaactc gccgcccgca gtggtgacga aatgcacgcc aaattccggc
    16381 gacaccgcct ggatgctgac ctcgatggcg ctggtgctga tgatgaccgt gccgggtctc
    16441 ggcctcttct atggcggcat ggtgcgcaag aagaatgtcg gcgacacggt gatgacgagc
    16501 ttcgccatcg cctgcctggt gacggtcatc tacgcggtcc tcacctacag cctcgccttc
    16561 accggcggct cggcctttat cggcgggcta agccgcgtct tcctgcaagg catcgtcagc
    16621 gacatcgcca acggcgtcgg caatccgaac ccgctcgcgc caaccattcc tgaaaccgtc
    16681 tatatgtgct tccagatgac cttcgcgatc atcacgccgg ccttgatcgc cggggccttc
    16741 gcggagcgca tgaaattctc ggcgatgctg tggttcgtcg gcctgtggtc
//
LOCUS       DATGJW010000134         6931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_438861, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000134 DATGJW010000000
VERSION     DATGJW010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6931
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_438861"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            362..2158
                     /locus_tag="VKU03_04035"
     CDS             362..2158
                     /locus_tag="VKU03_04035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HLJ70462.1"
                     /translation="MTRPAAPEEPAKRPTLAALRPLLPYALAYRGRVAGAFLSLLAAS
                     GSTLVVPIAVRRMIDYGFSADRPGLVNAYFSGMIAVVAVLAVASGLRYYFVMTLGELV
                     VARLRGDVFAHLTRLDASFFDAEKTGEIVSRLSADTTQLKSAFGSSASIALRNLFMFV
                     GAATMMIVTSPKLSAYVLIAIPFIVLPLYAAGRRVRNRARAAQDTLAAAAAFAAESLS
                     AVRVMQAFLAEAATLARFRAAAWGAYEAARATTQVRAFVTVAAIFLAFASVVVVLWLG
                     AKDVLAHRMTGGALSQFLLFAVLAAGALGELSQVWSEVSAASGAAGRIAELLRVQPKI
                     VAPAKPQALPSPARGLIAFERVVFAYPGRPDEPILKGLDLSVAPGEVVALVGPSGAGK
                     STVFQLAMRFYDPDRGAVSFDGVDIRKADPTDLRRRIAFVPQEPAIFGTTIAENIAYG
                     APDATPEAIREAARRAAADGFIAALPQGYDTLVGERGVTLSGGERQRIAIARAILKDA
                     PVLLLDEATSALDAENEALVQKALESLMAGRTTLVIAHRLATVIHADRILTIEAGRVV
                     EEGTHQSLLARGGLYARLARLQFEAGAQALAG"
     gene            complement(2182..3450)
                     /locus_tag="VKU03_04040"
     CDS             complement(2182..3450)
                     /locus_tag="VKU03_04040"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HLJ70463.1"
                     /translation="MSADASGAERAIRSGQSANSAVLRAGAALVLAAGIAASLHAWLT
                     FVQAFSDGSRYNAVGGDFMVFHAAARNYLDGHLATIFDGDRLTAFINEAYRSWLPMEL
                     RYRPWVYPPSYLLLLAPFGLLGFLASYAAFQVATATALVAALVVGAKRRPSAWIIAAA
                     ALLSPAAVDNIGSGQNAFLIAALLIAGFRLLDSRPVLAGMVLGLLTVKPQFALVAPIA
                     LVAAGQWRALLAAAGSAFFLVVVSAAILGVDAWNLWLQQVLSNLAASNAQWTRYGRLW
                     GDSTWTCAILLGASPVAASVAQWTSTLFAAGTVAVVFRRLSGETVRLVVILAATAFTA
                     PYWSFYDAVLLAVAGLLWAADRSAAEGAAWAWIVALALWLIPVASPPLLTPAGRLIPL
                     VIAIFLALSIRAPQVDATAKTSPLAGVAPL"
     gene            3622..4758
                     /locus_tag="VKU03_04045"
     CDS             3622..4758
                     /locus_tag="VKU03_04045"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HLJ70464.1"
                     /translation="MRVDSFSWTAAEGWRARPDPAADLVLYFGPRPLLEEGGFFAGLR
                     ARYPNARIAGCSTGGQIFGDDVEDDRLAGVAVGFDAARVKLHATRIAGAADSRRCGGE
                     IGEALRGDGLAGVLLLSDGLNVNGSDLAAGLVEAVGAAVPVSGGLAGDGPHFLRTLVG
                     ADAAPAEKRVAAIGFYGDKLRIGHGNAGGWSVFGPRRIITKAQGNVLHELNGEKALDL
                     YERYLGPEDSKDLPGSALLYPLRIGDPRHPDHDVVRTVLSVDRRAGSMTFAGNMPEGW
                     SAQLMRGHPDRLVAGASAAAQQARLADVAPGSALALMVSCIGRRLLMGQSVVGEVEAA
                     SEALGERYHKLGFYSYGEISPHRVTGRSVLHNQTMTVMTLAEAA"
     gene            4762..>6931
                     /locus_tag="VKU03_04050"
     CDS             4762..>6931
                     /locus_tag="VKU03_04050"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLJ70465.1"
                     /translation="MHPLLRKQLAKAAGAEGATDIDRLAASVSATYEDADKDRRRADR
                     SITLMIDELAELTEQLEQKVAARTAELEETRNTLSAALENVDQGIVMVDGDNRIQVCN
                     RRACQLLDLPLNLAEGAPAASAIIEAQIALQEFVGVDPALVESWRRTPLTDATPIYER
                     RRPNGAVLEVRTVKLPGGGAVRTFTDITERREREAQIARAEADYRSLFENAVIGVYRS
                     SLDGSRMMRANPALARLNGYETEAELLAAVGDIAGEWYVDPGRREQFRALIDANGRID
                     DFVSEVRRHRTRERIWVSESAWLVRSEKGEPLYYEGTVIDASQRILAEQRIAHMAHHD
                     ALTDLLTRAAFLDSLRAALRRAEPQRLVAVHCVDLDRFKDVNDTLGHPAGDQLLRNAA
                     KRLRAGLREKCEVARLGGDEFAVIQRDVADRAEAEALAWRLVRILSARYNIDGAAVHI
                     GASVGVILAPAGAEAEELMKNVDIALYRAKAAGGSTYRLFDPAMGEAAQRRRALEIDL
                     RGAVANNELEVYLQPIVDIADAAIVGFEALLRWRHPEQGMMPPAEFIPIAEETGLIGS
                     IGEWVLIEACAQAALAAGDFTISVNLSPAQFRSARVVRAVKAALAASGLAASRLVLEI
                     TESVLLMDNEQTKASLAELRRLGVRFALDDFGTGHSSLSYLQKFRFDTIKIDRSFVAS
                     IGADQVNAALVRAVVSLGRELGIAVVAEGIETEEQRETLAA"
BASE COUNT         1096 a         2432 c         2305 g         1098 t
ORIGIN      
        1 ttggcgagcg acgcgcgaag cgcgcttggc cttgacggcg agcacggctc aggcgcgccg
       61 cgcttcaaga tgtgtgcatg ccgtagttta taggcaggga agatgaccga ttgagaaatc
      121 ggccagctgt cgagtcggca aagcattacg actggcgctc cgcccgccga gcgcaattgg
      181 ggcgagcgct cgcggtttgc cggccggaat ttgcagcggc gcttttgcgc cgcggccatt
      241 cgttgcgttg acggcgccgc ctgatcggga ctaagcgcag gaggaattgt cgcccggcgc
      301 ggcctgtcgg cctaagccct tgatattcct ccgccggcaa gtcctggagt gcaaagcgtt
      361 catgacccgt cccgcggcgc cggaagagcc cgcaaagcgc cccacgctgg cggcattgcg
      421 gccgctcctg ccttacgcgc tcgcctatcg cggccgggtc gccggggcct tcttgtcgct
      481 tctcgccgcc tcgggatcga cgctggtcgt gccgatcgcg gtcaggcgga tgatcgatta
      541 cggcttctcc gccgaccggc ccggcctggt gaacgcctat ttcagcggca tgatcgcggt
      601 cgtcgccgtg ctcgcggtcg cctccggcct tcgctactat ttcgtcatga cgctcggcga
      661 gctcgtcgtc gcaagattgc gcggcgacgt cttcgctcat ctgacgaggc tcgacgcctc
      721 gttcttcgac gccgagaaga ccggcgagat cgtctcgcgc ctctccgccg acacgaccca
      781 gctcaaatcg gctttcggct cctcggcgtc gattgcgctg cgcaatctct tcatgtttgt
      841 cggcgcggcg acgatgatga ttgtcaccag cccgaagctt tcggcctatg tcctgatcgc
      901 cattcccttc atcgtcctgc cgctctatgc ggcgggccgg cgggtgagga accgcgcccg
      961 ggcggcgcag gatacgctcg ctgcggccgc cgctttcgct gccgagagcc tttccgccgt
     1021 ccgcgtcatg caggcctttc tcgccgaggc ggcgacgctg gcgcgcttcc gcgccgccgc
     1081 ctggggcgcc tacgaagcgg cgcgggcgac gacgcaagtg cgcgccttcg tcaccgtcgc
     1141 ggcgatcttt ctcgccttcg ccagcgtcgt cgtcgtgctg tggctcggcg ccaaggatgt
     1201 tctcgcgcac cgcatgacgg gcggcgcgct gtcgcagttc ctgctcttcg cggttctcgc
     1261 cgccggcgcg ctcggcgaat tgtcccaggt gtggagcgaa gtctcggccg cctccggcgc
     1321 ggccggccgc atcgctgaat tgttgcgcgt tcaaccgaaa atcgtcgcgc cggccaagcc
     1381 gcaggcgctg ccctcgccgg cgcggggcct tatcgccttc gagcgggtcg ttttcgccta
     1441 tccgggccgg cccgacgagc cgatcctgaa agggctcgat ctttccgtcg cgcccggcga
     1501 ggtcgtcgcc ttggtcgggc cgtccggggc cggcaagtcg acggtcttcc aactggcgat
     1561 gcgcttctac gatcccgatc gcggcgcggt ctcgttcgac ggcgtcgaca tccgcaaggc
     1621 cgatccgacg gacctgcgcc ggcgcatcgc cttcgtgccg caggagccgg cgattttcgg
     1681 aacgacgatc gccgagaaca tcgcctatgg cgcgcccgac gccacgcccg aggcgatccg
     1741 cgaggctgcc cggcgcgccg ccgccgacgg cttcatcgcc gctttgccgc agggctacga
     1801 cacattggtc ggcgagcgcg gcgtgaccct ttccggcggc gagcgccagc gcatcgccat
     1861 cgcccgggcg atcctgaagg atgcgccggt cctgctcctc gacgaggcga cctcggcgct
     1921 cgacgccgag aacgaggctc tggtgcagaa ggcgctcgaa tcgctgatgg ccggccgcac
     1981 caccttggtc atcgcccacc gcctggcgac cgtcatccac gccgaccgaa tcctcacgat
     2041 cgaggccggc cgcgtcgtcg aggaaggcac gcatcagagc ctgctcgccc gcggcggcct
     2101 ctacgcccgc ctcgcgcggc tgcaattcga agccggcgcg caggcgctgg cggggtagga
     2161 cgaggcggct cggtacagca gctaaagcgg cgcaacgccc gcgagcggcg acgtcttcgc
     2221 tgtcgcgtcg acctgcggcg cgcgaatcga aagcgcgagg aaaatcgcga tgacgagcgg
     2281 gatcagccga ccggccggcg tgagcagcgg cgggctagcg accggaatga gccagagcgc
     2341 cagcgcgaca atccacgccc aggccgctcc ttctgcggcg gacctgtcgg cggcccagag
     2401 caacccggcg accgcgagga ggacagcgtc gtagaaactc cagtaggggg ccgtgaacgc
     2461 cgtcgctgcc agaatgacga caagcctcac tgtctcgcct gacagccgcc taaacacgac
     2521 ggcgaccgtt ccggcggcga agagcgtaga ggtccattgc gcgacggacg ccgcaacagg
     2581 cgaggcgccc aacagaatcg cgcaggtcca ggtcgaatct ccccacaacc ggccgtatcg
     2641 ggtccactgc gcgttcgagg cggcgagatt cgacaggact tgctgcagcc acaggttcca
     2701 agcatcgacg cccaagatcg ccgcgctgac gacgaccaga aaaaacgccg atccggccgc
     2761 agcgagcaac gctcgccatt gtccggcggc gaccagcgcg atgggcgcaa cgagcgcgaa
     2821 ttgcggcttg accgtgagga ggcccaaaac catgccggca agtacgggcc ggctgtccaa
     2881 aagccggaac cccgcaatca gcagcgcagc gatcaggaac gcgttctgac ccgatccgat
     2941 attgtccaca gccgccgggg acagaagcgc agcggccgcg atgatccagg ccgacgggcg
     3001 cctcttcgcg ccgacgacga gcgccgcgac cagcgctgtg gcggttgcaa cctgaaacgc
     3061 ggcataggag gcgaggaagc caagcaggcc gaaaggcgcg agcagcagca gatagcttgg
     3121 cgggtagacc caaggtcggt accgaagctc catcggcagc caggagcgat aagcttcatt
     3181 gatgaaggcg gtgagccgat cgccgtcgaa aatcgtcgcc agatggccgt cgaggtagtt
     3241 tcgcgcggcg gcgtggaaga ccataaaatc gccgccgacc gcgttgtagc ggctgccgtc
     3301 gctgaacgct tgaacgaagg tcagccaggc gtgcagtgag gcggcgatcc ccgccgccaa
     3361 gaccagcgcc gcgcccgcgc gtagaacggc gctgttcgcg ctctggcccg agcgtatggc
     3421 ccgctctgcg ccgcttgcat ccgcactcac ggtcgaccct acgtcgtccc ctgcctcgcc
     3481 gatcctagag gcggcccgtt aacaaaccct cccgtcgaaa agcggagcgt ccggccgggt
     3541 aggcctcctg tttaccgagc gcgttacagg cttcttaaga tcatcccctt tatcgtcgca
     3601 gcgtttcacg ctgggcgtct catgcgcgtc gatagcttct cctggacggc ggccgaaggc
     3661 tggcgggcgc ggcccgaccc cgccgccgat ctcgtcctct atttcgggcc gcgacccctc
     3721 ctcgaagaag gcggtttctt cgccggcctt cgcgcccgct atccgaacgc gcgcattgcc
     3781 ggctgctcca ccggcggaca gattttcggc gacgatgtcg aggatgaccg cctcgccggc
     3841 gtcgccgtcg gtttcgacgc cgcgcgggtc aagctccatg cgacccggat cgcgggcgcc
     3901 gcggattcgc gccgttgcgg cggcgagatc ggcgaagctt tgcgcggcga cggccttgcc
     3961 ggcgtgctgc tcctctccga cgggctcaac gtcaatggca gcgatttggc ggccgggctg
     4021 gtcgaggcgg tcggcgcggc ggttccggtt tccggcgggc tcgccggcga cggcccgcat
     4081 ttcttgcgca ccctggtcgg cgccgacgcc gcgcccgcgg agaagcgcgt cgcggcgatc
     4141 ggcttctacg gcgacaagct gcgcatcggc cacggcaatg ccggcggctg gagcgtgttc
     4201 gggccgcgcc gcatcatcac caaggcgcag ggcaacgtgc tccacgaact caatggcgag
     4261 aaggcgctcg atctttacga gcgctatctc ggcccggaag attccaagga tctgcccggc
     4321 tcggcgctgc tctatccgct gcgcatcggc gatccgcgcc atcccgatca cgatgtggtg
     4381 cgcaccgtgc tcagcgtcga ccgccgggcc ggctcgatga cgttcgccgg caacatgccg
     4441 gaaggctgga gcgcgcaatt gatgcgcggc catcccgacc ggctggtcgc cggcgcctcg
     4501 gccgccgcgc agcaggcgcg tctcgccgac gtggcgcccg gctcggcctt ggccctgatg
     4561 gtcagttgca tcggccggcg cctgctgatg ggccagagcg tggtcggcga ggtcgaggcg
     4621 gcgagcgaag cgctcggcga gcgctaccac aagctcggct tctattccta tggcgagatt
     4681 tcgccgcacc gcgtcaccgg ccgctcggtc ctgcacaatc agacgatgac ggtgatgact
     4741 ctcgccgagg ccgcctgaca aatgcacccg ctgctgcgca aacagctcgc gaaagcggcc
     4801 ggcgcggagg gcgcgacgga tatcgaccgc ctggcggctt cggtcagcgc gacctacgag
     4861 gacgccgaca aggaccggcg gcgcgccgac cgctcgatca ccttgatgat cgacgaactc
     4921 gccgaactca ccgagcaatt ggagcagaag gtcgcggcgc gcacggcgga gctcgaagaa
     4981 acccgcaaca cgctgagcgc cgcgctggag aatgtcgacc agggcatcgt catggtcgac
     5041 ggcgacaatc gcattcaggt ctgcaaccgc cgcgcttgcc agcttctcga tctgccgctc
     5101 aacctcgccg agggcgcgcc cgccgcctcg gcgatcatcg aggcgcagat cgccttgcag
     5161 gaattcgtcg gcgtcgatcc ggctctggtc gaatcctggc gtcggacgcc gctgacggat
     5221 gcgacgccga tctatgaacg gcgccggccg aatggcgccg ttctcgaagt gcgcaccgtc
     5281 aagcttccgg gcggcggcgc cgtccgcacc ttcaccgata tcaccgagcg gcgcgagcgc
     5341 gaggcgcaga tcgcccgcgc cgaagcggac tatcgcagcc tgttcgagaa cgcggtgatc
     5401 ggcgtctatc gctcgagtct cgacggaagc cggatgatgc gcgccaatcc ggctttggcg
     5461 cgcctcaacg gctatgagac ggaagccgag ctgctcgccg cggtcggcga catagccggc
     5521 gaatggtacg tcgacccagg ccgccgggag cagtttcgcg cgctgatcga cgccaatggc
     5581 cggatcgacg atttcgtctc ggaagtccgc cgccaccgca cgcgcgagcg catctgggtc
     5641 tcggaatcgg cctggctggt gcgctccgag aaaggcgagc cgctttatta cgaaggcacg
     5701 gtcatcgacg ccagccagcg catccttgcc gagcagcgca tcgcccatat ggcccaccac
     5761 gacgcgctga ccgatcttct gacccgcgcc gcgttcctcg attcgctgcg cgccgccttg
     5821 cgccgcgccg agccgcaacg cctcgtcgcg gtccactgcg tcgatctcga ccgcttcaag
     5881 gacgtgaacg acacgctcgg ccatccggcg ggcgatcaat tgctgcgcaa cgccgccaag
     5941 cgcctcaggg ccggcctgcg cgaaaaatgc gaggtcgcgc ggctcggcgg cgacgaattc
     6001 gcggtcatcc agcgcgacgt cgccgaccgc gccgaggcgg aggctctggc ctggcggctc
     6061 gtccggattc tctccgcccg ctacaatatc gacggggccg cggtccatat cggcgccagc
     6121 gtcggcgtca ttctcgcgcc ggccggcgcc gaagccgaag agctgatgaa gaatgtcgac
     6181 atcgcgctct accgcgccaa ggccgcaggc ggctcgacct accgtctgtt cgatccggca
     6241 atgggcgaag cggcgcagcg ccggcgggcg ctggagatcg atctgcgcgg cgcggtggcg
     6301 aacaacgagc tcgaagtcta tctccagccg atcgtcgaca tcgccgatgc ggcgatcgtc
     6361 ggcttcgagg cgctgctgcg ctggcgccat cccgaacaag gcatgatgcc gccggccgaa
     6421 ttcatcccga tcgccgaaga gaccggcttg atcggctcga tcggcgaatg ggttctgatc
     6481 gaagcctgcg cgcaagcggc tttggccgcc ggcgatttca ccatctcggt caatctgtcg
     6541 ccggcgcaat tccgcagcgc ccgggtggtg cgggcggtca aggccgcgct cgccgcctcc
     6601 ggcctcgccg cgtcgcgtct cgtgctcgaa atcactgaat ccgtcctcct gatggacaat
     6661 gagcagacca aggcctcgct cgccgaactg cgccgcctcg gcgtgcgctt cgcgctcgac
     6721 gatttcggca ccggccattc ctcgctcagc tacctgcaga aattccgctt cgacacgatc
     6781 aagatcgacc gatccttcgt cgcctcgatc ggcgccgacc aggtcaacgc cgccctggtc
     6841 cgcgccgtgg tctcgctcgg ccgcgaactg ggcattgcgg tcgtcgccga agggatcgag
     6901 accgaggagc agcgcgagac gctcgccgcg c
//
LOCUS       DATGJW010000135        16213 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_854855, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000135 DATGJW010000000
VERSION     DATGJW010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 16213)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16213)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16213
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_854855"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..619
                     /locus_tag="VKU03_04055"
     CDS             <1..619
                     /locus_tag="VKU03_04055"
                     /inference="COORDINATES: protein motif:HMM:NF015682.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HLJ70466.1"
                     /translation="IEPFRGVPLSFLCDAMGGDGALDWRIKPICSPSLFGVALTCQCG
                     PADVLPLKTAVAMCQPGDVIVVATGGFTGTAVVGDLLLGVAKNRGAAGFVTDGLIRDR
                     VDVEALQLPVYAIGVTPNSPHSKGPGSVGLPIVCGGRTIASGDVILGDRDGIVAVPRA
                     EIVAVLRNLERVKAMEAKASAAVRGGSKNVEDVTPLTPDERIRWV"
     gene            841..1698
                     /locus_tag="VKU03_04060"
     CDS             841..1698
                     /locus_tag="VKU03_04060"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLJ70467.1"
                     /translation="MIDFPIIDSHIHLLDQKRFGYSWAAGAPKLNRDWTADDLAAYAK
                     PYRIEGFVFVEVDVDMPQYLDEAKWVEGLAAKDKRVKGCVACLPLEKGKAIEPEMAKI
                     AKLSIVRGVRRLIQNQPDPEFVLRPAFLDALKLLPKYNLLFDICIFHHQLPNTLEMVR
                     RCPDVSFVLDHIAKPGIKAGLIEPWRGQIKTLAALPNVVCKLSGVTTEADHAAWTRDQ
                     LRPYLDHVIESFGPDRIMFGGDWPVSELAGTYRQWVETLDWATSGMSAGDKRKLFRDN
                     AIKTYRLSL"
     gene            1698..2900
                     /locus_tag="VKU03_04065"
     CDS             1698..2900
                     /locus_tag="VKU03_04065"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HLJ70468.1"
                     /translation="MDKAALRRRGYSVRALRALARKELPRMIFDMVDGAAGDENTMRR
                     NEAVLAGIEFVPKLLAGAPRRDQSVELFDLKLPSPVIVGPTGLAGLLWPQAEIATARA
                     AARFGTVYTTSHASTSTIEEIGAASAGPKWMQVFLFKDRGVTAEFAERAKAAGYQGLV
                     LTVDNQVTAGRDRDLKNNMAFPLHFTPLNALDLLSRPGWLWRMRRTPQPTAVNYGARM
                     KIGAFGALMAEQLDPAVAWRDMEWLRGIWRGPLLIKGVLHPDEALGAQRRGADAIIVS
                     NHGGRQLDGAIASIAALPAIADALGGAIPVLIDGGFRRGIDVLKAIALGARAVLLGRP
                     HLWGVACAGEDGAYWALELFRREIDRAMALGGWDSLAALDRSVLSAPTIPFSPKRGEK
                     VAVRSTAG"
     gene            complement(2961..3746)
                     /gene="recO"
                     /locus_tag="VKU03_04070"
     CDS             complement(2961..3746)
                     /gene="recO"
                     /locus_tag="VKU03_04070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HLJ70469.1"
                     /translation="MEWRDEGVVLGVKKHGESSVIVELLTRAHGRHLGLVRGGRSARF
                     HSLLQPGNSLQLVWRARLDEHLGAYAVEPLALRAGRLMGSALALHGLSYLGALIRLLP
                     ERDPHGALHEALIVVVDHLDAAEVAPALIARFEARILAECGFGLDLERCAASGARDNL
                     VYVSPKSGRAVSAEAGAPWRDKLLPLPSFLRGEEGDAPPAADVADAYRTTGYFLERDL
                     FGPRGLPMPDSRRAFLAAADAARVPSPRDAGRRWPEGPDEGLR"
     gene            complement(3760..4110)
                     /locus_tag="VKU03_04075"
     CDS             complement(3760..4110)
                     /locus_tag="VKU03_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase accessory protein"
                     /protein_id="HLJ70470.1"
                     /translation="MSPPNPPFSTERPDEPVFAAPWEAQAFALAVLLNQKGVFGWDEW
                     TRTLAEEIHRPETEARRYYEHWLAALERLAAEKELAASAELAARRDAWERATRATPHG
                     RPILLENDPLARRG"
     gene            complement(4097..4756)
                     /gene="nthB"
                     /locus_tag="VKU03_04080"
     CDS             complement(4097..4756)
                     /gene="nthB"
                     /locus_tag="VKU03_04080"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit beta"
                     /protein_id="HLJ70471.1"
                     /translation="MIGGQDLGGMMGFGRIAAEANEPLFHAEWERRAFAITLAAGALS
                     RWSIDQSRHAREARPPAEYLASNYYELWLKGLEKLLLENGLVTAEEIAAGRSLAAPKP
                     VGRVLKAEHVAAALAKGSRADRPLAKPARFGAGDSVRTKALYTTGHTRLPRYAFGKLG
                     RIEAVRGGFVLPDSAAAGQGEAPEWLYTVRFAGRELWGEAADENLAVSIDAWESYLEP
                     A"
     gene            complement(4753..5379)
                     /gene="nthA"
                     /locus_tag="VKU03_04085"
     CDS             complement(4753..5379)
                     /gene="nthA"
                     /locus_tag="VKU03_04085"
                     /EC_number="4.2.1.84"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008837012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018822 - nitrile hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0050898 - nitrile metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrile hydratase subunit alpha"
                     /protein_id="HLJ70472.1"
                     /translation="MHDHDDHEHDHGADEEMALRVKALESLLVEKGYVDTAALNALID
                     TYETKVGPRNGAKVVAKAWAEPDFLAWLRRDATAAIASLGFTGRQGEHMVAVENTPET
                     HNLVVCTLCSCYPWPVLGLPPAWYKSPPYRSRAVRDPRGVLAEFGVELPEGVAVRVWD
                     STAEIRYLVVPMRPPGTEGWPAERLERLVTRDSMIGAGLPLGAEALGA"
     gene            complement(5435..6637)
                     /locus_tag="VKU03_04090"
     CDS             complement(5435..6637)
                     /locus_tag="VKU03_04090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70473.1"
                     /translation="MSGIARGGREFVRTMAALFSGGFSTFALLYSAQPLLPAFAREFS
                     ISPATSSLALSATTGTLAFAMLIAGTISDVFGRKRVMVASLAASSAIMILAAFAQGWS
                     ELVALRALTGLALSGLPAVAMAYLADEMAANAIGLAMGLYIAGNTVGGLAGRLVGAVS
                     ADHGSWRDGFALIGVVGLLSVVLFAKALPASRAFTPVRPHLSALARSMVSHFADPGLR
                     LLFAIGFLVMGCFVTVYNYIGFRLLKPPFSLSQTQVGLIFILYLLGGASSAMMGDLAG
                     RIGRRRVLWIAMAIMLAGLAATLFDDLRLIIAGVGLITIGFFGAHSIASSWVGLRAEK
                     AKAQAASLYLLFYYLGSSLVGTLGGVFYARGGWPGVASLVGGLTLAGVAVALRLARIP
                     PPKWMAKN"
     gene            complement(6801..7724)
                     /gene="era"
                     /locus_tag="VKU03_04095"
     CDS             complement(6801..7724)
                     /gene="era"
                     /locus_tag="VKU03_04095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase Era"
                     /protein_id="HLJ70474.1"
                     /translation="MSETACGFVALIGAPNAGKSTLINALVGAKIAAVSRKAQMTRAP
                     ARGIVAEGAAQIVFVDTPGLFAPKRRLDRAMTRAAWDAADDADCIVLLIDAKAELRAG
                     PHAALSEETEAILDALSKRKPAKILALNKIDAVERSSLLGLAAKLNERLGFDETFMIS
                     ALTGDGLGELRKALAQRMKPGPWLYPEDQISGGDLRSLAAEITREKLFERLHDELPYQ
                     ATVETEQWKDLPDGSARIEQTIYVTRESHRKIVLGEGGRTIKAIGSAARKEIAEANEA
                     PVHLFLFVKVRENWTEDPERYRAMGLEFPKS"
     gene            complement(7721..8437)
                     /gene="rnc"
                     /locus_tag="VKU03_04100"
     CDS             complement(7721..8437)
                     /gene="rnc"
                     /locus_tag="VKU03_04100"
                     /EC_number="3.1.26.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004525 - ribonuclease III activity
                     [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease III"
                     /protein_id="HLJ70475.1"
                     /translation="MAKAERAKASLADLEAKLAYRFSDPSLAERALTHLSAARSDSAS
                     SRLESYQRLEFLGDRVLGLAIAEMLYEAYPQASEGELSIRLAALVRREACAEAAAHWD
                     LGPHLRLGAGEAQAGGRKKATILADVCESLIGAVFLDGGFAAARDLIKRAWGERVSEA
                     ATPARDAKTVLQEWAQARALGAPSYDEVERSGPDHAPKFTVQVTLAGYPPELGSASSK
                     RAAEQAAAQAFMDRLESAKP"
     gene            complement(8439..9248)
                     /gene="lepB"
                     /locus_tag="VKU03_04105"
     CDS             complement(8439..9248)
                     /gene="lepB"
                     /locus_tag="VKU03_04105"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HLJ70476.1"
                     /translation="MTEPMRLKGEGVDASVKKSEEGGLWEIVKVILQAVAIAIVIRTL
                     LFQPFNIPSGSLIPTLDIGDYLFVSKYSYGYSHFSLPSFLDWFSNEWHGRILASQPKR
                     GDVIVFRLPRDETTDYIKRLIGLPGDKIQMVHGRLVINGQMVERQPLPPYHITDRSGH
                     DIAVPHYLETLPGGVKHEIIQIEGDDGFYSNTDVYTVPPGHYFMMGDNRDNSTDSRVP
                     EDRDGVGYVPFENLIGKAQIIFFSVKDGYPAWEFWEWPWTVRWNRLFQPVH"
     gene            complement(9467..10540)
                     /locus_tag="VKU03_04110"
     CDS             complement(9467..10540)
                     /locus_tag="VKU03_04110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLJ70477.1"
                     /translation="MRKLMTAAAVAALTLAFAGAAEAAAKVKACWVYVGPVGDFGYSY
                     QHDQGRQYAAKELGDKVDASTYLENVPENDSERAFEQLARSGCNIIFGTSFGFMDPMV
                     KVAKKFPNVKFEHATGYKRLPNLATYAARFYEGRYVMGQIAGKMTKTNTIGYIVSFPI
                     PEVIQGINSFELGLQSVNPNAKIKIVWVSTWFDPGKEADAAKALIAGGADILTQHTDS
                     AAPLQEAEKAGKLGFGQSSDMIKFAPKAQLTANTDNWGPYYVERIKAVIDGTWKSQDT
                     WDGIGKGMVKLAPFTNMPDDVKAMAEKTTADIGSGKLQIFACPVLDQAGKDQCKPGAK
                     TLDDGQILGMNWYVKGVDDKMPQ"
     gene            complement(10681..11610)
                     /locus_tag="VKU03_04115"
     CDS             complement(10681..11610)
                     /locus_tag="VKU03_04115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011088898.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70478.1"
                     /translation="MTFDAQQVQDVLIIVVTAATPLLIAAVGELVAERSGVLNLGVEG
                     MMIIGAATGFAAAYSTDSTLVGVAAGVVSGAILAAIFAFLTLGLAANQVASGLALTIL
                     GRGVANLIGAGFVGLKRTPAPHLYIPGLTDIPLVGKLFFGEDFFVYFALLLTGGVAYW
                     LIRTRRGLTLRAVGDNHFSGHSLGVPVRRIRLLAVLFGGGCAGLAGSYLSLAYTPFWS
                     PDMTAGRGWIALALVVFASWKPWRAFAGALLFGGVTVLQLVFQTWGVPIAPQFLASLP
                     YLTTIAVLVVLSIRDIRGAAAPASLGLAFIPDR"
     gene            complement(11607..12692)
                     /locus_tag="VKU03_04120"
     CDS             complement(11607..12692)
                     /locus_tag="VKU03_04120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70479.1"
                     /translation="MRIDLEARPKRSTLREILSPVIALIVAVLVGGAVVAIMGRSPLT
                     AFDVYFVQPLSQFYSLQAIAVKASPLILIAVGLAFCYRANLWNIGAEGQFIAGGALGG
                     WLGLLTHDGAYQAIGSWWILPAMILLGILGGVLYAMIPAALKVWLNVSEILTSLMLVY
                     VAQLTLDWLVRGPWKDPHGFNFPVTVNFDPEATLPILFDGGTLHLGVLFAPVVVIVAA
                     IVFSKTIFGFQVRLAGSSPKAARFGGFNDKRIAFAAFAISGGLAGLAGIAEVSGKIGQ
                     LLTTISPGYGFSAIIVAFLGRLSPVGIFIGGLVLAVTFVGGEDAQIALKLPGDLTLAF
                     QGVLLICVLAADVLARYRLRLRLGAES"
     gene            complement(12682..14187)
                     /locus_tag="VKU03_04125"
     CDS             complement(12682..14187)
                     /locus_tag="VKU03_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70480.1"
                     /translation="MAVRNITKRFGPFTANDRVNLDLVQGEVHALLGENGAGKSTLVK
                     MLYGILEPTEGEILWRGAPIRLPNPNAARALGIGMVFQHFSLFENLTVAENIALAMAP
                     EIKLDQVPAKIAEISRAYGMGLEPSRPVWTLSAGERQRIEIARCLLQDPKVLILDEPT
                     SVLTPQEAEKLFETLERLKAEGRALLYISHHLEEVQRLCDRATILRLGKVVGACNPRV
                     ETPRSLAALMVGSEIKEVRPAGAHALGKERLVVANLSLPSPELHGVALKNISAAVKGG
                     EIFGVAGVAGNGQSEFFAALSGETLARDPNAIVIDGAPVGALGIGARRALNAGFVPEE
                     RNGHAAAPDFTLSDNIVLSGHASGDMTRAGFINAGLAKEKSRKVIAAFDVRAAAPDPL
                     ARTLSGGNLQKFVVGREILREPSVLVVNQPTWGVDVAAAATIRQALLDLCAKGAAVLI
                     ISQDLDELFEICDRIAVIHTGELSAPLPARSTTREEVGLLMAGMGGGGHAH"
     gene            complement(14384..15352)
                     /locus_tag="VKU03_04130"
     CDS             complement(14384..15352)
                     /locus_tag="VKU03_04130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinone oxidoreductase"
                     /protein_id="HLJ70481.1"
                     /translation="MRAIRVHEVGGPEAMRLEDVQLPPPGPGEARVRHRAIGLNYIDV
                     YYRTGHYPSQAPFTPGSEGAGDVVAVGPGVKGFKLGDRVAYVSRTGAYAEEANVAVTA
                     LVRLPQAISYETAAAAMLKGLTAQYLLRQTYRVKKGDTILVHAAAGGVGLILCQWGKA
                     LGATVIGTVGSPDKAAMAKKAGAAHTILYREEDFAARVVEITKGAKCHVVYDGVGKAT
                     FPASLDCLRPLGMFASYGSASGAIENFNLALLAQKGSLFATRPSLFTFLADRARYERM
                     ARDLFSVLRSNAVKVKIGARLPLAEAARAHEALEARATSGSTVFLP"
     gene            15633..16142
                     /gene="accB"
                     /locus_tag="VKU03_04135"
     CDS             15633..16142
                     /gene="accB"
                     /locus_tag="VKU03_04135"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxyl carrier
                     protein"
                     /protein_id="HLJ70482.1"
                     /translation="MKKPQSKTSAAASEGLQSTLDLSIIAALAKIAGQHDLSEVEIEH
                     QGLRVRVARERAAPALAGLVAPTPVAAAVIPAPGAAPEPAPGATPADHPGVIKSPMVG
                     TVYRRASPEAKPFVEIGSVVKVGDKLLLVEAMKTFNEILATKAGKVVEIFVEDATPVE
                     FGQPLLAIA"
BASE COUNT         2957 a         5351 c         5332 g         2573 t
ORIGIN      
        1 gatcgagccg tttcgcggcg ttcctttgag ctttctctgc gacgcgatgg gcggcgacgg
       61 cgcgctcgac tggcggatca agccgatctg ctcgccctcg ctcttcggcg tcgcgctgac
      121 ctgccaatgc gggccggccg acgtgctgcc gctcaaaacg gcggtcgcca tgtgccagcc
      181 gggcgacgtg atcgtcgtcg cgaccggcgg ctttaccggg acggccgtgg tcggcgacct
      241 cttgctgggc gtcgccaaga atcgcggcgc ggccggcttc gtcaccgacg ggctgatccg
      301 cgaccgggtc gatgtcgaag ccttgcagct tcccgtctac gcgatcggcg tgacgccgaa
      361 ctcgccgcat agcaaggggc cgggctcggt cggcctgccg atcgtctgcg gcggccggac
      421 catcgcttcg ggcgacgtga tcctcggcga ccgcgacggg atcgtcgccg tgccgcgcgc
      481 cgaaattgtc gcggtgctga ggaatctgga gcgcgtcaag gcgatggagg ccaaagcctc
      541 ggccgcggtg cgcggcggct cgaagaatgt ggaggacgtg acgccgctga cgccggacga
      601 gcgcatccgc tgggtttagg gcgcggccgg cggtcgcgcc ccttctccct gcaaagcgga
      661 gagaaggtgg cccgaagggc cggatgaggg caacgcagga ctctgaaagt acgcaccttc
      721 tgtctcgcta atcccatagc tcagcgcagc ccctcacccg gcgcttcggg ccaccctctc
      781 cccgctgtgc ggggcgaggg ggattgccca gcttaaaatt gacgtaccaa ggaaacgccc
      841 atgatcgact tccccatcat cgactcgcat attcatctgc tcgaccagaa gcgcttcggc
      901 tattcctggg cggccggcgc gccgaagctc aatcgcgact ggacggcgga cgaccttgcc
      961 gcctacgcca agccgtaccg gatcgagggc ttcgtcttcg tcgaggtcga tgtcgacatg
     1021 ccgcaatatc tcgacgaggc gaaatgggtc gagggcctcg ccgccaagga caagcgcgtc
     1081 aaaggctgcg tcgcgtgcct gccgctcgaa aaaggcaagg cgatcgagcc ggaaatggcg
     1141 aagatcgcca agctctcgat cgtgcgcggc gtgcggcggc tgatccagaa ccagcccgat
     1201 ccggaatttg tcttgcggcc ggctttcctc gacgcgctca agctgctgcc caaatacaac
     1261 ctcctcttcg acatctgcat cttccaccat cagcttccga acacgctcga aatggtccgc
     1321 cgctgcccgg atgtttcctt cgtgctcgac catatcgcca agccgggaat caaggccgga
     1381 ctgatcgagc cttggcgcgg tcaaatcaag acgctcgccg cgcttccgaa cgtcgtctgc
     1441 aagctttccg gcgtgacgac ggaagccgat cacgccgcct ggacgcgcga tcagcttcgc
     1501 ccctatctcg accatgtcat cgaaagcttc ggccccgacc ggatcatgtt cggcggcgat
     1561 tggccggtct cggaactcgc cggaacctat cggcaatggg tcgagacgct cgactgggcg
     1621 acgagcggca tgagcgcggg cgacaagcgg aagctgttcc gcgacaatgc gatcaagacc
     1681 taccggctga gcctgtgatg gataaggcag cgctcaggcg tcgcggctac agcgtccgcg
     1741 ccttgcgcgc gctggcgcgc aaagagctgc cgcgcatgat cttcgacatg gtcgacggcg
     1801 cggcgggcga tgaaaacacg atgcggcgca acgaggcggt cctcgccgga atcgagttcg
     1861 tacccaagct cctcgccggc gcgccgcggc gcgatcagag cgtcgaattg ttcgacctga
     1921 agctcccctc cccggtcatt gtcggcccga ccggccttgc cggcctgctc tggccgcaag
     1981 ccgaaatcgc gacggcgcgg gcggcggcgc gattcgggac ggtctatacg acgagtcacg
     2041 cttcgacctc gacgatcgag gagatcggcg cagcgtcagc cggtccaaaa tggatgcagg
     2101 tcttcctgtt caaggatcgc ggcgtcaccg ccgaattcgc cgagcgcgcc aaggccgccg
     2161 gctatcaagg cctcgtcctt accgtcgaca atcaggtgac ggccggccgc gaccgcgacc
     2221 tgaagaacaa catggccttc ccgctgcatt ttaccccgct gaacgcgctc gatcttttga
     2281 gccggcccgg ctggctctgg cggatgcggc ggacgccgca gccgacggcg gtcaattacg
     2341 gcgcgcgcat gaaaatcggc gcgttcggcg cgctgatggc cgagcagctc gatcccgccg
     2401 tcgcctggcg cgacatggaa tggctgcgcg ggatttggcg cggcccgctc ctgatcaagg
     2461 gcgtgctgca tcccgacgag gcgctcggcg cgcagcggcg cggcgccgat gcgatcatcg
     2521 tctccaatca cggcgggcgc cagctcgacg gcgcgatcgc ctcgatcgcg gctttgccgg
     2581 cgatcgccga tgcgctcggc ggcgcaatcc cggtgctgat cgacggcggc tttcgccgtg
     2641 ggatcgacgt actgaaagcg atcgcgctcg gcgcccgcgc cgtgctgctc ggccggccgc
     2701 atctgtgggg cgtcgcgtgc gccggcgaag acggcgcgta ctgggcgctc gagctgttcc
     2761 gccgcgagat cgaccgggcc atggcgctgg gcggctggga ctcgctcgct gcgctcgacc
     2821 gcagcgtact ttccgcgcca acgatcccct tctccccgaa aaggggggag aaggtggccg
     2881 tgcgcagcac ggccggatga ggggcagcgc cgaaatcgga aggtacgcgc gcgctgcttc
     2941 cgattcgtca atcggcacgt tcagcgcagc ccctcatccg gcccttcggg ccaccttctc
     3001 cccgcgtcgc ggggagaagg gacgcgcgcc gcgtctgcgg cggcgaggaa ggcgcgccgc
     3061 gaatccggca tcggcaggcc gcgcgggccg aacaggtcgc gctcaaggaa atagcccgtc
     3121 gtccggtagg cgtcggcaac gtccgcggcc ggcggcgcgt cgccttcctc gccgcgcagg
     3181 aatgacggca gcggcagcag cttgtcgcgc cagggcgcgc cggcctcggc gctgaccgca
     3241 cggccggatt tcggcgagac atagacgaga ttgtcgcgcg cgcccgaggc ggcgcagcgt
     3301 tcgagatcga ggccgaagcc gcattcggcg agaatgcgcg cctcgaaccg cgcgatcaaa
     3361 gccggcgcga cctccgccgc gtcaagatga tcgacgacga cgatcagcgc ctcatgcagg
     3421 gcgccgtgcg gatcgcgctc cggcaggagc cggataagcg cgccaaggta cgataggcca
     3481 tgcagcgcga gcgcgctgcc catgagccgt cccgcccgca aggccagcgg ctcgacggca
     3541 taggcgccga gatgctcgtc gagccgcgcc cgccagacga gttgcagcga attgcccggc
     3601 tgcaaaaggc tgtggaagcg agccgagcgc ccgccgcgca ccaggccgag atggcggcca
     3661 tgcgcgcgcg tcaaaagctc gacgatgacc gagctctcgc catgcttctt gacgccgaga
     3721 acgacgcctt cgtcgcgcca ttccattttc cgtccgcctt catccgcgcc gcgcgagcgg
     3781 atcgttctcc aaaaggatcg gccggccatg cggcgtcgcc cgcgtcgcgc gctcccaggc
     3841 gtcgcgccgc gccgcgagct ccgccgacgc agcgagctcc ttttcggcgg ccagccgctc
     3901 gagcgcggcg agccaatgct cataatagcg ccgcgcttcg gtctccggcc ggtgaatttc
     3961 ctcggccagc gtccgcgtcc attcgtccca gccgaacacg cccttctggt tcaacagaac
     4021 cgcgagcgcg aaggcctgcg cctcccaagg cgcggcgaaa acaggctcgt cggggcgctc
     4081 cgtcgaaaac ggcgggttag gcgggctcaa gataactctc ccaggcgtcg atcgagacgg
     4141 cgagattttc atccgcggct tcgccccaga gctcgcggcc ggcgaagcgg accgtataga
     4201 gccattccgg cgcttcgccc tgccccgccg cggccgaatc cggcagcacg aagccgccgc
     4261 ggaccgcctc gatgcggccg agcttgccga aagcgtagcg cggcagcctc gtatggccgg
     4321 tcgtataaag cgctttcgtc cgcacgctat cgccggcgcc gaagcgggcc ggttttgcca
     4381 gcggccgatc ggcgcgcgac cctttcgcca gagccgcggc gacatgctcc gctttcaaaa
     4441 cccggccgac cggctttggc gccgccaagc tgcggccggc ggcgatctct tcggccgtga
     4501 cgagcccgtt ctcgagcagc agcttctcca ggcccttcag ccagagttcg taatagttcg
     4561 aggccaaata ttccgccggc ggccgcgcct cgcgggcatg gcgggactgg tcgatgctcc
     4621 accggctcaa agcgccggcg gccagtgtga tggcgaaggc gcggcgctcc cattcggcgt
     4681 ggaagagcgg ctcgttcgct tcggccgcga tgcggccaaa gcccatcatg ccgccgagat
     4741 cttgcccgcc gatcatgcgc cgagcgcctc cgcgcccagc ggcaggcccg cgccgatcat
     4801 cgaatcgcgg gtgaccaatc gctccaagcg ctcggccggc cagccctcgg ttccgggcgg
     4861 ccgcatcggc acgacgaggt agcggatttc cgcggtcgaa tcccagacgc ggacggcgac
     4921 gccctcgggc aactcgacgc cgaattcggc gagcacgccc ctcggatcgc gcacggcgcg
     4981 cgagcgatag ggcggcgatt tgtaccaggc cggcggcagg ccgagcaccg gccagggata
     5041 gcaggagcag agcgtgcaga cgacgaggtt atgcgtctcg ggcgtattct cgaccgcgac
     5101 catgtgctcg ccctggcggc cggtaaagcc gagcgaggcg atggcggcgg tcgcgtcgcg
     5161 ccgcagccag gcgaggaaat ccggctcggc ccaagccttg gcgacgactt tggcgccgtt
     5221 gcgcggaccg accttcgtct cgtacgtatc gatcagggcg ttcagcgccg ccgtatcgac
     5281 ataacctttt tcgaccagca gcgattccag cgccttgacg cgcagcgcca tctcttcgtc
     5341 ggcgccgtgg tcgtgttcat ggtcgtcgtg atcatgcatg gctcagtctt tctacgctcg
     5401 gcccatctta gagcaaagca cggcgccgga tttgttaatt tttggccatc catttcggcg
     5461 gcgggatgcg cgccagcctc agcgccaccg cgacgccggc cagggtcagc ccgccgacca
     5521 ggctcgcgac gcccggccag ccgccccgcg catagaagac cccgcccaaa gtcccgacga
     5581 ggctggagcc gagatagtag aacaaaagat agagcgaggc ggcctgcgcc ttggcctttt
     5641 ccgcgcgcag cccgacccag ctcgacgcga tcgaatgggc gccgaaaaag ccgatcgtga
     5701 tcagaccaac gccggcgatg atcaatcgga ggtcgtcaaa aagggtcgcc gcgagtcccg
     5761 ccagcatgat cgccatggcg atccacagaa cgcgccgccg cccgatccgg ccggcaagat
     5821 cgcccatcat cgccgagctc gcgccgccga gcagatagag gatgaagatc aagccgactt
     5881 gcgtctggct caaggagaaa ggcggcttga gcaggcgaaa gccgatgtaa ttgtagaccg
     5941 tcacgaaaca gcccatgacc aggaagccga tcgcgaacag caggcgcaag ccgggatcgg
     6001 cgaaatggct gaccatggag cgcgccaggg cgctgagatg cggccgcacc ggggtgaagg
     6061 cgcgggaggc cggcagcgct ttcgcgaaca ggacgacgct caacagcccg acgacgccga
     6121 tcaatgcgaa gccgtcgcgc caggagccgt gatccgccga aaccgcgccg accaggcgcc
     6181 cggcaaggcc gccgacggtg ttgccggcaa tgtagagccc catcgcgagg ccgatcgcat
     6241 tagccgccat ctcgtcggcg agataggcca tggcgacggc aggcaagccg gacaaagcca
     6301 ggccggtcag cgcgcggagc gcgacgagct cgctccaccc ttgagcaaag gcggcgagga
     6361 tcatgatcgc cgaggaggcg gcgagcgagg cgaccatcac ccgcttgcgg ccgaacacgt
     6421 cggaaatcgt cccggcgatc agcatcgcga aagcgagcgt tccggtggtc gcggaaagcg
     6481 ccaggctgct cgtcgccggc gaaatggaga attcgcgcgc gaaagccggc aggaggggct
     6541 gcgcgctata gagcagcgcg aaggtcgaga agccgccgga aaagagcgcg gccatcgtgc
     6601 ggacgaattc gcgtccgccg cgggcgatgc cagacatttg ttgcggacgt ggctgcaatg
     6661 aggagatcag gcgaaattct gtccctggtg aactttgtgc gtccttcgtg tccttggtgt
     6721 tgaaatcgaa aactcgcaaa gcgctaaaca cgaagatcac aaaggaagca caaagatcac
     6781 gaagagaccg agccgtctcc tcaactcttg gggaattcga ggcccatcgc gcgatagcgc
     6841 tcaggatcct cggtccaatt ctcgcgcact ttgacgaaga gaaagaggtg caccggcgcc
     6901 tcattggcct cggcgatctc cttgcgcgcc gccgagccga tcgccttgat ggtgcggccg
     6961 ccttcgccga gcacgatctt gcgatgcgat tcgcgcgtca catagatcgt ctgctcgatc
     7021 cgcgccgatc cgtcgggcag atccttccat tgctcggtct cgacggtcgc ttggtacggc
     7081 aattcgtcat gcagccgctc gaacagcttt tcgcgggtga tctcggcggc gaggctgcgc
     7141 agatcgccgc cggaaatctg gtcctccgga tagagccagg ggccgggctt cattctctgc
     7201 gccagcgcct tgcgcaattc gccgagcccg tcgccggtca aagccgaaat catgaacgtc
     7261 tcgtcgaacc ccaagcgttc gttgagcttg gcggcgaggc cgagcaaaga ggaccgttcg
     7321 accgcgtcga tcttgttcag cgccaggatt ttcgccggct tccttttcga cagagcgtcg
     7381 agaatggcct cggtctcctc gctcagggcc gcgtgcggcc cggcccgcag ctccgccttg
     7441 gcgtcgatca acaggacgat gcaatcggcg tcatccgctg cgtcccaggc cgccctggtc
     7501 atggcgcggt caagccgccg tttcggcgcg aacaggcccg gcgtgtcgac gaagacgatc
     7561 tgcgccgcgc cctcggcgac gatgccccgc gccggcgccc gcgtcatctg cgccttgcgc
     7621 gacacggcgg cgattttggc gccgacaagc gcgttgatca gcgtcgattt gccggcgttc
     7681 ggcgcgccga tcagggcgac gaagccgcat gcggtctcgc tcatggcttg gcgctttcga
     7741 gccgatccat gaaggcttgc gccgcggctt gctcggcggc gcgtttcgaa gaggccgagc
     7801 cgagttccgg cggatagccg gccagcgtca cctgcaccgt gaatttcggc gcatgatccg
     7861 gcccggaccg ctcgacctcg tcataggagg gcgcgccgag cgcgcgggcc tgcgcccatt
     7921 cctgcaggac ggttttggcg tcgcgcgccg gcgtggcggc ctcgctcacc cgctctcccc
     7981 aggcgcgttt gatcaggtcg cgcgcggcgg cgaagccgcc gtcgagaaag acggcgccga
     8041 tcagggattc gcagacatcg gcgaggatcg tcgccttctt gcgcccgccc gcctgcgctt
     8101 cgccggcgcc gagacgaaga tgcgggccga ggtcccaatg cgccgcggcc tcggcgcagg
     8161 cttcccggcg caccaacgcc gccagccgga tcgacaattc gccttcgctc gcctgcggat
     8221 aggcttcgta gagcatttcg gcgatggcga ggccgagcac gcggtcgccc aaaaactcca
     8281 gccgctggta ggattcgaga cgactgctcg cggaatccga gcgcgccgcc gacaaatggg
     8341 tcagcgcgcg ctcggcgagc gagggatcgg aaaaacgata ggcgagcttg gcctcgagat
     8401 cggcaagcga agctttcgct cgctctgctt tcgccattct agtgaaccgg ctggaacagc
     8461 cggttccacc gcacggtcca cggccactcc cagaactccc aggccgggta gccgtccttg
     8521 accgagaaga agatgatctg ggccttgccg atcagatttt cgaacggcac atagccgacg
     8581 ccgtcgcgat cctccggaac gcgtgaatcg gtcgagttgt cgcgattgtc gcccatcatg
     8641 aagtagtggc cgggcggcac ggtgtagaca tcggtgttgg agtagaagcc gtcatcgccc
     8701 tcgatctgga tgatctcgtg cttgacgccg ccgggcagcg tttcgaggta atgcggcacc
     8761 gcgatatcgt gtccggaccg atcggtgatg tgatagggcg gcagcggctg gcgctcgacc
     8821 atctggccgt tgatgaccag gcgaccatga accatctgaa tcttatcgcc cggcaggccg
     8881 atcaggcgct tgatgtaatc ggtcgtctcg tcgcgcggca ggcggaagac gatcacgtcg
     8941 ccgcgcttcg gctgcgaggc caaaatcctg ccatgccatt cgttggaaaa ccaatcgagg
     9001 aaggagggca gcgaaaagtg cgaatagccg taggaatatt tcgagacgaa gagatagtcg
     9061 ccgatatcga gggtcgggat cagcgagccg gaagggatgt tgaacggctg gaatagcaag
     9121 gtgcggatga cgatggcgat cgccaccgct tgcaggatga ccttgacgat ctcccacagg
     9181 ccgccttctt cgctcttctt gacggacgca tccacgcctt cgcctttcaa tcgcatcggt
     9241 tcggtcatcg gctggtcaag ccttccacta aagtcgcggc gccggcgccg tgtttcgcaa
     9301 ggcgacctat acgcgctggc cgcccgcccg ccaagcgcct agcgaaccgg agcgcggcgc
     9361 gacacgaacg cgcaggcgcc gctaaaaggc ccgccttgct ccaagaaaaa cgaggcggcc
     9421 cgaaggccgc ctcgttgcgt tcggcaaggc ttcttccgaa cagaccttat tgcggcatct
     9481 tgtcgtcgac gcctttcaca taccagttca tgcccaaaat ctggccgtcg tccagcgtct
     9541 tcgcgccggg cttgcactga tccttgccgg cctggtcgag gaccggacag gcgaaaatct
     9601 gcagcttgcc ggagccgata tcggcggtgg tcttctcggc catggccttc acgtcgtcag
     9661 gcatattggt gaacggcgca agcttgacca tgcccttgcc gatgccgtcc cacgtgtcct
     9721 gggatttcca ggtcccgtcg atcacggcct tgatccgctc gacgtaatag ggaccccaat
     9781 tgtcggtgtt ggccgtcaac tgggccttag gcgcgaactt gatcatgtcc gacgactggc
     9841 cgaagccaag cttgccggcc ttctcggcct cctgaagggg cgcggcggag tcggtgtgct
     9901 gggtgaggat gtccgcgccg ccggcgatca aagccttggc cgcgtccgcc tccttgcccg
     9961 gatcgaacca ggtgctgacc cagacgatct tgatcttggc gtttggattg accgattgca
    10021 ggcccagctc gaacgagttg atgccctgaa tgacttccgg gatcgggaac gagacgatgt
    10081 agccgatcgt gttggtcttg gtcatcttgc cggcgatctg gcccatcaca tagcggcctt
    10141 catagaatcg ggcggcgtag gtggcgaggt tcggcaggcg cttgtagccg gtcgcatgct
    10201 cgaactttac gttggggaac ttcttggcga ccttcaccat cggatccatg aagccgaagg
    10261 acgtgccgaa gatgatgttg cagcccgacc gcgccaattg ctcgaaggcg cgctcgctgt
    10321 cgttctccgg cacgttttcg agataggtcg aagcgtcgac cttgtcgccg agctccttgg
    10381 ccgcatattg gcggccctga tcgtgctgat aggaatagcc gaaatcgccg acaggcccga
    10441 catagaccca gcaggctttc accttggccg ccgcctcggc ggcgccggcg aaagccagcg
    10501 tcagagccgc gacagccgcg gccgtcatca atttgcgcat gtaactctct cccgtttcat
    10561 tccgaccatt gcactgccgg cggccgtatc cggcttaggt tcacccggct gggccgcctg
    10621 tcaaacccct gtcccagcta tagaaagcgc ggcccctcca agcaacaaaa agcgtttgta
    10681 ttagcgatcc ggaatgaagg cgagcccgag ggaagccggc gccgccgcgc cgcgaatgtc
    10741 gcggatcgac agcacaacca gcacggcgat cgtcgtcaaa taaggcaaag aggcgaggaa
    10801 ttgcggcgca atcggcacgc cccaggtctg gaagacgagc tgcagcacgg tgacgccgcc
    10861 gaacagcagc gcgccggcaa aggcgcgcca cggcttccac gaggcgaaga cgacaagcgc
    10921 gagggcgatc cagccgcggc cggcggtcat gtccggcgac cagaacgggg tataggccag
    10981 cgaaagataa gagcccgcga ggccggcgca gccgccgccg aacagcaccg cgagcagccg
    11041 gatgcggcgg accggcacgc cgagcgaatg gccggaaaag tgattgtcgc cgaccgcccg
    11101 gagagtcagg ccccgcctgg tgcggatcag ccaataggcg acgccgccgg tcagcagcag
    11161 agcgaaatag acgaagaaat cctcgccgaa gaagagcttg ccgaccaaag gaatgtcggt
    11221 gaggccggga atgtagagat gcggcgccgg ggtccgcttc aagccgacaa agccggcgcc
    11281 gatcagattg gcgacgccgc ggccgagaat ggtcaaagca aggcccgacg cgacctgatt
    11341 ggccgcgagg ccgagcgtca gaaaggcgaa aatcgccgcg aggatcgcgc ccgagacgac
    11401 gccggcggcg acgccgacca aggtcgaatc cgtcgaatag gcggcggcga agccggtcgc
    11461 cgcgccgatg atcatcatgc cctcgacgcc gagattgagc acgccggagc gctcggcgac
    11521 caattcgccg accgccgcga tcagcaacgg cgtcgccgcc gtgaccacga tgatcagcac
    11581 atcctgaact tgctgcgcgt cgaacgtcac gattcagccc ccagtcgcag gcgaagccga
    11641 tagcgggcga gcacgtcggc ggcgagcacg cagatcagca gcacgccttg gaaggccagc
    11701 gtcagatcgc cgggcagttt gagcgcgatc tgcgcgtctt cgccgccgac aaaggtcacg
    11761 gcaagaacca ggccgccgat gaaaatgccg accggcgaca gccggccgag aaaggcgacg
    11821 atgatcgccg agaagccata gcccggcgag attgtcgtca ggagctggcc gatcttgccg
    11881 gagacttcgg cgatgccggc gaggccggcg agtcctccgg aaatggcgaa ggcggcgaag
    11941 gcgatgcgct tgtcgttgaa gccgccgaag cgcgccgctt tcggcgagga gccggcgagg
    12001 cgcacctgaa agccgaaaat cgtcttcgaa aagacgatcg ccgcgacgat caccacgacc
    12061 ggcgcgaaga gaacgccgag atgcagcgtg ccgccgtcga aaaggatcgg caaggtcgcc
    12121 tcgggatcga aattcaccgt gaccgggaaa ttgaagccgt gcgggtcctt ccacgggccg
    12181 cgcaccagcc agtcgagcgt gagctgggcg acatagacca gcatcaggct ggtgaggatt
    12241 tcggagacgt tcagccagac tttgagcgcc gccggaatca tcgcataaag gacgccgccg
    12301 agaatgccga gcaggatcat cgccggcaaa atccaccacg agccgatcgc ctgataggcg
    12361 ccgtcatggg tgaggaggcc gagccagccg ccgagcgcgc cgccggcgat gaattgtccc
    12421 tcggcgccga tattccagag attggcgcga tagcagaagg cgaggccgac cgcgatcagg
    12481 atcagcggcg aggctttcac cgcgatcgcc tgcagcgagt agaattgcga caaaggctgg
    12541 acgaaataga cgtcgaaagc ggtgagcggc gagcggccca tgatcgccac caccgcgccg
    12601 ccgacgagca ccgcgacgat cagggcgatc acgggcgaca gaatttcgcg taaggtcgag
    12661 cgtttcggcc gcgcttcgag atcaatgcgc atggccgccc ccgcccatgc cggccatcag
    12721 caacccgacc tcctcgcgcg tcgtcgagcg cgccggcagc ggcgccgaca attcgccggt
    12781 atgaatgacg gcgatgcggt cgcagatttc gaacaattcg tcgaggtcct ggctgatgat
    12841 caggaccgcg gcgcctttcg cgcaaagatc gagcagcgcc tggcggatcg tcgcggccgc
    12901 cgcgacatcg acgccccagg tcggctggtt gacgacgagg accgagggct cgcgcaggat
    12961 ttcacggccg acgacgaatt tctgcaaatt gccgccggac agagtgcgcg ccagcggatc
    13021 gggcgcggcc gcgcgcacgt cgaaagcggc gatgaccttg cgcgactttt ccttggcgag
    13081 accggcattg atgaagccgg cgcgcgtcat gtcgccggag gcgtggccgg aaaggacgat
    13141 attgtcggac agggtgaagt ccggcgcggc ggcgtggccg ttgcgctcct cgggcacgaa
    13201 gccggcgttc agggcgcgcc tggccccgat ccccaaagcg ccgactggcg cgccgtcgat
    13261 gacgatcgcg ttgggatcgc gggccaaggt ctcgccggaa agggccgcga aaaattccga
    13321 ctggccgttg ccggcgacgc cggcgacgcc gaaaatctcg ccgcccttca ccgcggccga
    13381 gatattcttc agggcgacgc cgtgcagttc gggcgagggc aggctcaaat tggcgacgac
    13441 cagccgttcc ttgccgagcg catgcgcgcc ggccggccgc acttccttga tttccgagcc
    13501 gaccatgagg gcggcgagcg agcgcggcgt ctcgacccgc ggattgcagg cgccgaccac
    13561 cttgccgagg cgcaagatcg tcgcgcgatc gcaaaggcgc tgcacttctt cgagatggtg
    13621 cgaaatgtag agcagcgccc tgccctcggc tttcaagcgc tccagagtct cgaacagctt
    13681 ttcggcctct tgcggcgtca ggaccgaggt cggctcgtca aggatgagga ctttcgggtc
    13741 ttgcagcagg cagcgcgcga tctcgatgcg ctggcgctcg ccggccgaaa gcgtccaaac
    13801 cggccgcgat ggttcgaggc ccatgccata ggcgcgggat atttccgcga ttttcgccgg
    13861 cacttgatcg agtttgatct ccggcgccat ggcgagcgcg atattttccg cgacggtcag
    13921 gttctcgaaa agcgagaaat gctggaagac catgccgatg ccgagcgccc gagcggcgtt
    13981 cggattgggc aggcggatcg gcgcgccccg ccaaagaatt tcgccctcgg tcggctcgag
    14041 aatgccgtag agcattttaa ccagggtcga cttgcctgcc ccgttctcgc ccagcaaggc
    14101 gtgaacctcg ccctgaacga gatcgagatt gacccgatcg ttcgccgtga aaggtccgaa
    14161 ccgcttggtg atgttgcgaa ccgccaataa cggcgtcgtt tcgcccgaac cccgccctgt
    14221 tccggccgcg tcaagttgcg ccaaactcag cctcccagcc aagattgagg ccgattttag
    14281 cgggtcgttt gaatagagcc agcgcgaaag agcctggact ttgcgaaaat tttgccaagc
    14341 actttcgcgc cgcgtgacga tcttgacgag gccgcaacgg cggttacggc aggaacacgg
    14401 tcgagccgct tgtcgcgcgc gcttcgagcg cctcatgcgc tcgggcggct tcggccagcg
    14461 gcagacgcgc gccgattttc accttcaccg cgttcgagcg cagcacgctg aaaaggtcgc
    14521 gcgccatgcg ctcgtagcgg gcgcgatcgg caaggaaggt gaagaggctt ggccgcgtcg
    14581 cgaacagcga gcccttctgc gccaacagcg cgaggttgaa attctcgatc gcgcccgaag
    14641 ccgagccgta agaggcgaac atgcccaggg gacgtaggca atcgagcgaa gcggggaagg
    14701 tcgctttgcc gacgccgtca tagacgacat ggcatttcgc gcctttggtg atctcaacga
    14761 cccgcgccgc aaaatcctcc tcgcgataaa gaatggtgtg cgcagcgccg gcctttttgg
    14821 ccatcgccgc cttgtccggc gagccgacgg ttccgatcac cgtcgcgccg agcgccttgc
    14881 cccattggca aaggatcagc ccgaccccgc ccgccgcggc atggacgagg atcgtgtcgc
    14941 cctttttcac gcgataggtt tgccggagga gatattgcgc ggtcagccct ttcaacatcg
    15001 ccgccgccgc cgtctcgtag gaaatcgcct ggggcagacg gaccagcgcc gtcacggcga
    15061 cattggcctc ttccgcgtaa gcgccggtgc gcgatacata agcgactcgg tcgccgagct
    15121 tgaagccctt gacccccggg ccgaccgcga ccacatcgcc ggcgccttcg ctgcccgggg
    15181 tgaacggagc ttgcgacgga tagtgaccgg tccggtaata gacatcgata tagttgagcc
    15241 cgatggcgcg atgcctgacc cgcgcttcgc cggggccagg cggcggcaat tgaacgtctt
    15301 caagccgcat cgcctcaggc ccaccgacct cgtggacccg gatcgcgcgc atgaagccct
    15361 ccgccccttg aaaagaccgc atcatccgcc ggccattctg caaccgcaag agcgaaagac
    15421 gcccgcttat caggggatga ctcaatcgcg cgccctttcc ctcgccgccc gatttggcta
    15481 taggaccggc tctccttaaa gcggcgatcg gccgccaatc cctagccgcc gacggtgagc
    15541 agagttgtga aacgccccga gccgacgctt ttgcgagcaa accggccctc caattgcgtc
    15601 cttgccgaca agaaggggcg aacggggcct ttatgaaaaa accgcaaagc aaaaccagcg
    15661 ccgccgcgag cgaggggctg caaagcacgc ttgacctttc gatcatcgcg gcgctcgcca
    15721 agatcgccgg ccagcacgac ctcagcgagg tcgaaatcga gcatcaaggc ttgcgcgtca
    15781 gagtggcgcg cgagcgggcg gcgcccgccc ttgccggcct ggtcgcgccg acgccggtcg
    15841 ccgcggccgt catcccagct cctggagcgg cgccggagcc cgcacccggc gcgacgccgg
    15901 ccgaccatcc cggcgtcatc aaatcgccga tggtcggcac ggtttaccgg cgcgccagcc
    15961 cggaggcgaa gcccttcgtc gagatcggct cggtcgtcaa agttggcgac aagctgctgc
    16021 ttgtcgaggc gatgaagacg ttcaacgaga ttcttgcgac gaaggcgggc aaagtcgtcg
    16081 aaatcttcgt cgaagacgca acgcccgtgg aattcgggca gcctttgctc gccattgcgt
    16141 aaccggcgcg tcgtcgttcc gccgtgtagg gtcgattcat gttcgataag atcctgatcg
    16201 ccaatcgcgg cga
//
LOCUS       DATGJW010000136         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_336894, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000136 DATGJW010000000
VERSION     DATGJW010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_336894"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..464)
                     /locus_tag="VKU03_04140"
     CDS             complement(<1..464)
                     /locus_tag="VKU03_04140"
                     /inference="COORDINATES: protein motif:HMM:NF022605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2937 family protein"
                     /protein_id="HLJ70483.1"
                     /translation="MIIRRLAVAIGLLFAAIGAQWPEFAQQYRQRLGGALDELNRMIA
                     EFDQQAQSQKLTREQGLARLERNDDPLARQRAEAINEDVERARRLSQQKEAFAQAGPV
                     ARLIALIRNFDPATMGQAWRDFEPALPVTPEAFVIGAGGLIVGWSLTHVVAWP"
     gene            614..1984
                     /locus_tag="VKU03_04145"
     CDS             614..1984
                     /locus_tag="VKU03_04145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase family protein"
                     /protein_id="HLJ70484.1"
                     /translation="MAIRTPAKIAACFAAMALTAGSAAAQDAPTPVPGLDKINHIIVV
                     YLENRSFDNLYGLFSGADGIDKAGAAAIQVDRDGKPYDKLPPVLNTEFRPPIIDTRFP
                     TDLPNRPFRSEPYAGLEHMTGDARHRYYQEQLQIDGGKMDKYVAWTDAGALVMSYFDG
                     SAAPLWRYAKDFTLMDHFHHAGFGGSFFNHVYMICACAPRYDNAPASLVAELDAKGDL
                     VKDGAVTPDGYAVNTLLPVNGPHPAKINPNFLLPVQTDATIGDRLDEKGVDWAWYSGG
                     WDAAVAGHPGPLFQFHHQPFAYFAHYALGTAGAKAHLKDETDFIKGIEKGELPPVVFF
                     KPSGEDNEHPGYSSVLAGEYHTALLVRLIQQSPLWKDSLIIVTYDENGGLWDHVAPPV
                     IDRWGPGTRVPAILISPFAKKGFIDHKVYDTTAILKLIETRYGLRPLGTRDAASGDLT
                     DALDLQ"
     gene            2139..2669
                     /gene="mog"
                     /locus_tag="VKU03_04150"
     CDS             2139..2669
                     /gene="mog"
                     /locus_tag="VKU03_04150"
                     /EC_number="2.7.7.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin adenylyltransferase"
                     /protein_id="HLJ70485.1"
                     /translation="MEPIPIGVVTVSDRASQGLYEDKGGPGIEAALAAILSTPWRPAR
                     RLVPDERPAIEAALRELADSEKCSLILTTGGTGPAPRDVTPEATQAVCERLLPGFGEL
                     MRTKSLLDVPTAILSRQLAGTRGSSLIVNLPGKPSAIRTCLDAVFPAIPYCLDLIGGA
                     RLETNPDACKAFRPKG"
     gene            2679..2930
                     /locus_tag="VKU03_04155"
     CDS             2679..2930
                     /locus_tag="VKU03_04155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70486.1"
                     /translation="MADERDIETLKELGAGRPAPESVAALYRQAFAQFGVKFLWSRKP
                     SDRPTIAQALVVAESLRREGSMQARPLAAQIEEACRAAL"
BASE COUNT          546 a         1030 c          975 g          531 t
ORIGIN      
        1 ggccaggcga cgacatgggt gaggctccag ccgacgatca atccgccggc gccgatgacg
       61 aacgcctccg gcgtcaccgg caaagccggc tcgaaatcgc gccacgcctg acccatcgtc
      121 gccggatcga aattgcggat cagcgcgatc agccgcgcca ccggcccggc ttgggcgaag
      181 gcctcctttt gctgtgacag gcgccgggcg cgctcgacat cctcgttaat cgcctcggcg
      241 cgctggcgag cgagcggatc gtcattgcgc tcgagccgcg ccaggccttg ctcgcgggtc
      301 agcttttggc tctgggcctg ctggtcgaac tcggcgatca tgcggttgag ttcgtcgagc
      361 gcgccgccga ggcgctggcg atattgctgc gcgaattctg gccattgcgc gccgatcgcg
      421 gcgaagagaa ggccgatcgc gacagcgaga cgacggataa tcacacagcc tccggcttgc
      481 ctcccggccg cgacgcgacg cggccgaagc aacgctaatg caggtcgccg gccctgtcat
      541 cgaggcgacg cgccgaagtt gacgacgccc ggcaaatcgc tccctatagc ccgcaaataa
      601 gggagcaggg cctatggcaa ttcgtacgcc ggccaaaatc gccgcgtgct tcgcggccat
      661 ggctttgacc gccggttcgg cggcggcgca agacgcgcca acgccggttc ccggtctcga
      721 caagatcaat catatcattg tcgtctatct cgagaatcgc agcttcgaca atctttatgg
      781 ccttttctcc ggcgctgacg ggatcgacaa agccggcgcg gcggcgatcc aggtcgatcg
      841 cgacggcaag ccttacgaca agctgccgcc ggtgctgaac accgagttcc ggccgccaat
      901 catcgatacg cgcttcccga ccgatctgcc gaaccgtcct ttccgctccg agccctatgc
      961 cgggctcgaa cacatgaccg gcgacgcgcg gcatcgctac tatcaggagc aattgcagat
     1021 cgacggcggc aagatggaca agtacgtcgc ttggaccgac gccggcgccc tggtgatgag
     1081 ctatttcgac ggctcggccg cgccgctctg gcgctacgcc aaggacttca cgttgatgga
     1141 ccatttccat cacgccggct tcggcggctc tttcttcaac catgtttaca tgatctgcgc
     1201 ctgcgcgccg cgctacgaca atgcgccggc ttcgctcgtc gcggagctcg acgcgaaggg
     1261 cgacctcgtc aaggatggcg cggtcacgcc ggacggctat gcggtcaaca ccttgctgcc
     1321 ggtgaacggt ccgcatccgg cgaaaatcaa tccgaatttt ttgctgccgg tccagaccga
     1381 cgcgacgatc ggcgataggc tcgacgagaa aggcgtcgac tgggcctggt attccggcgg
     1441 atgggacgcg gccgtcgcgg gtcatcccgg accgctcttc cagttccatc atcagccctt
     1501 cgcctatttc gcgcattacg cgctcgggac ggccggcgcg aaagcgcatc tgaaagacga
     1561 gaccgatttc atcaagggaa tcgaaaaagg cgaattgcct ccggtcgtct tcttcaagcc
     1621 gagcggcgag gacaacgagc atcccggcta ttcgagcgtg ctcgccggcg aatatcacac
     1681 cgcgctgctg gtgcggctca tccagcaaag cccgctctgg aaggacagcc tgatcattgt
     1741 aacctatgac gagaatggcg gcctttggga tcacgtcgcg ccgcccgtca tcgatcgttg
     1801 gggccccgga acgcgcgtcc cggcgatcct gatttcgccc ttcgccaaga aaggcttcat
     1861 cgatcacaag gtctatgaca cgaccgcgat cctgaagctg atcgaaacgc gctatggcct
     1921 tcggccgctc ggaacgcgcg atgcggcttc cggggacctc acggacgcgc tcgatctgca
     1981 gtaggatgta accggggcca atttccaggc gctgccgtcg ccgcggtcgc cgaccgcagc
     2041 aggcgcgaag ccctgtttcg ccctggagag gcgcggtcag cgaccgcgcc gacctgcccg
     2101 ccctaaaaat ttgtcccggt tactggtagg atttcagcat ggagccaatt ccgatcggcg
     2161 tggtgacggt ctcggaccgg gcgagccagg ggctttacga ggataaaggc ggccccggaa
     2221 tcgaggcggc gctggccgcg atcctgtcga cgccatggcg gccggcgcgc cgcctggttc
     2281 cggacgagcg gccggcgatc gaagcagcgc tccgcgagct cgccgacagc gagaagtgct
     2341 cgctgatcct gaccaccggc ggcaccggcc ccgcgccgcg cgacgtgacg ccggaggcga
     2401 cgcaagccgt ttgcgagcgc ctgttgccgg gtttcggcga gttgatgcgg acgaaaagcc
     2461 tgcttgacgt gccgaccgcg atcctgtcgc gccagctcgc cggcacgcgc ggttcatccc
     2521 tcatcgtcaa cctgcccggc aagcccagcg ccatccgcac ctgcctcgac gccgtctttc
     2581 cagcgatccc ctattgcctc gacctgatcg gcggcgcgcg gctggagacg aatccggacg
     2641 cctgcaaggc gttccggccg aagggatagc gaaaagcgat ggcggacgaa cgcgacatcg
     2701 aaaccttgaa ggagctcggc gcgggccggc cggcgccgga gagcgtcgct gcgctttatc
     2761 gtcaagcctt cgcgcaattc ggcgtcaagt ttctttggag tcgcaagccc tcggaccgcc
     2821 ccaccatcgc tcaggctttg gtcgtggccg agagcttgcg tcgtgaaggg agcatgcagg
     2881 cccgtccgct ggcggcgcaa atcgaagagg cctgccgtgc cgctctctaa gatccagacg
     2941 gaggtccttt ccctcctcgc cgctcatcga aatcctgaga gctgcgttgc gggcgccgcc
     3001 gcgctcaatc gcgacggacc gcgatactct gacgatgtgg atatctttca tgatcgcgaa
     3061 gagagcgtcg ctctcgcggc gg
//
LOCUS       DATGJW010000137         4023 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_232783, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000137 DATGJW010000000
VERSION     DATGJW010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4023)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4023)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4023
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_232783"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..463)
                     /locus_tag="VKU03_04160"
     CDS             complement(<1..463)
                     /locus_tag="VKU03_04160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083873.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HLJ70487.1"
                     /translation="MLRWSIATVSMGGALEGKLAAAAKAGFRAVEIFENDLTFFTGKP
                     RDVRLMAADLGLEIIALQPLRDFEAMPDPARARNFERAARKFDLMHELGARLLCLCSN
                     VSEEAIDDPARAAADLAELADRARQHGFRIGYEALAWGRRVKDWMQAWAIVE"
     gene            642..1361
                     /locus_tag="VKU03_04165"
     CDS             642..1361
                     /locus_tag="VKU03_04165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ70488.1"
                     /translation="MNVTGDGRNGGATLAETLHERIRQDIILGALQPAAKLKLEKLSK
                     SYGVSVNTLRESLSRLAADGLVVAEGQKGFAVLPVSMEDLREITEMRQLLECHALRQS
                     IANADLDWEARLVGAYHKLSKVEALVEAEPDLYGDAWERYNQEFHAALIANCRSRWLK
                     LFHKAMYDHSQRYRMLSLRTKPFPRSQSAEEHRQILDAAIARDVDTAERVLASHILKG
                     AELTGLEAPAGPAKLRTEPRR"
     gene            1522..2379
                     /locus_tag="VKU03_04170"
     CDS             1522..2379
                     /locus_tag="VKU03_04170"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HLJ70489.1"
                     /translation="MASAYPESVLLGLIGAPIAHSASPAMHEAAAAALGLRCFYHLIE
                     VAGADARRLRVMLDGLAALKFAGVNVTYPYKEAVVPLLDELAPSAAKVGAVNLIAVRG
                     ERLIGFNTDCSGFARALKRVIGDIRGQAVALIGAGGVGKAIGVALAEAGAGDIRIVDP
                     VPGKAATLAEALGAARACAGAADALDGAAGLVNASAVGMLPSRDSPVPRALLRRELWV
                     ADAVYQPLWTPLLAAARDKGAATMTGRELAVDQAIDAFEIFTGRTASRAVLEQAFDEV
                     IRRRDAALT"
     gene            2450..3598
                     /locus_tag="VKU03_04175"
     CDS             2450..3598
                     /locus_tag="VKU03_04175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ70490.1"
                     /translation="MIKRSASLGIAVLASFLAAGAAAQAAETINLADVAELSGSGATV
                     GTNWKNGVDLAVEEINAHGGILGAKIVVTHADSQSNAGIAKAQVQKALDGEPYALLGP
                     GYSGSVKVCEPLAAEAGIAEIMGGEAAELTRMGNKYVFRTSFGQQSSMPKIAKYIAEN
                     LKAKTAAIVYVNNDFGRGGHDMILKEFAARGVKVALDDSTEAGQADFAADALKVKNAN
                     PDVAFIYLNEEESARMLKELRKADVKAPLVGETTLIGQKVIELAGDAANGAIGHVGLT
                     TDAPVPALKAYGERFYAKYHYVPDHNGIKGYLAVYMIKAATEKMGKLDSKGLAAALHG
                     LTITTAMEPNILMDVSIDQNGDLDRQSFIGKVIDGKQTITEVLPKLKD"
     gene            3800..>4023
                     /locus_tag="VKU03_04180"
     CDS             3800..>4023
                     /locus_tag="VKU03_04180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70491.1"
                     /translation="MANFIALLIAGLATGALYSLVAIGFTLLWQTSQTINFAQGEFVM
                     LPAFFMLAAMNVHLPFWAAILIGLALSMALL"
BASE COUNT          726 a         1295 c         1317 g          685 t
ORIGIN      
        1 cctcgacgat ggcccaggct tgcatccagt ccttgacgcg gcgcccccag gcgagcgcct
       61 cgtagccgat gcgaaagccg tgctggcggg cgcggtcggc gagctcggca aggtcggcgg
      121 cggcgcgggc gggatcgtcg atcgcctcct ccgaaacgtt cgagcagagg cacaggaggc
      181 gcgcgccgag ctcgtgcata aggtcgaact tgcgcgcggc gcgctcgaaa ttgcgggcgc
      241 gggccggatc gggcatggcc tcgaaatcac gcaagggctg gagcgcgatg atttccaggc
      301 cgagatcggc ggccatcagc ctcacgtcgc gcggcttgcc ggtgaaaaag gtaaggtcgt
      361 tctcgaaaat ctcgaccgcc cggaaccccg ccttggccgc cgccgcgagc ttgccctcca
      421 gcgcgccgcc catgctgacc gtggcgatgg accatctcag catttttcag aaatctccta
      481 tatttttatt ttcatagagt atatgctcgg ggcgagccag tcacgttccc gtcattgcga
      541 gcgcagcgac gaagcaatcc cttcgtaggg cggcgctcta cgaagggatt gcgccgtttc
      601 gctcgcaatg acgaggtcgg gtacaagcga gggcggtacg gatgaacgtt acgggcgatg
      661 gccggaacgg cggcgcgact ttggccgaga cgctgcatga gcgcatccgc caggacatca
      721 tcctcggcgc gctgcagccc gcggccaagc tcaagctgga aaagctcagc aagagctacg
      781 gcgttagcgt caacacgctg cgcgaatcct tgtcccgcct cgccgccgac gggctggtcg
      841 tcgccgaggg ccagaagggt ttcgccgttc tgccggtctc gatggaggat ttgcgcgaaa
      901 tcaccgagat gcgccagctc ctcgaatgcc acgctctgcg ccagtcgatc gccaacgccg
      961 atctcgactg ggaggcccgc ctggtcggcg cgtatcataa gctgtcgaaa gtcgaggcgc
     1021 tggtcgaggc cgagccggac ctctatggcg acgcctggga gcgctataat caggagttcc
     1081 acgccgcgct gatcgccaat tgccgctcgc gctggctgaa actcttccat aaggcgatgt
     1141 acgaccacag ccagcgctac cgcatgctgt cgctgaggac caaacccttc ccgcgctcgc
     1201 aatcggccga ggagcaccgc cagattctcg atgcggcgat cgcccgcgat gtcgacacgg
     1261 ccgagcgcgt cctggcgagc catatcctca aaggggcgga attgaccggg ctcgaagcgc
     1321 cggccggacc ggccaagctc aggaccgagc cgcgccgata aataatggat attttgattt
     1381 ctattgaata ttgacacaca tttgtcaatg ccctatccgt cattgcgagg agcgaagcga
     1441 cgaagcaatc cattcgtcga gcccggcccg acgaatggat tgcttcgctc cgcccgcaat
     1501 gacggaaggt tttttgcatc cgtggcgagc gcctatcccg aaagcgtcct gctcggcctg
     1561 atcggcgcgc cgatcgccca ttcggcctcg cccgccatgc atgaggcggc ggccgcggcg
     1621 ctcggtttgc gctgcttcta ccatctcatc gaggtcgccg gcgccgatgc gcgtcgcctg
     1681 cgagtcatgc tcgacggcct cgccgcgctg aaattcgccg gcgtcaatgt gacttatccc
     1741 tataaggaag cggtcgtacc tttgctcgac gagcttgcgc cgtcggcggc aaaggtcggc
     1801 gcggtcaacc tgatcgccgt ccgcggcgag cggctgatcg gcttcaacac cgattgcagc
     1861 ggcttcgccc gggcgctgaa gcgcgtcatc ggcgatatcc ggggccaggc ggtcgccttg
     1921 atcggcgccg gcggcgtcgg caaggcgatc ggcgtcgccc tcgccgaggc gggcgccggc
     1981 gatatccgca tcgtcgatcc ggttcccggc aaggccgcaa cgctcgccga ggcgctcggg
     2041 gccgcgcgcg cttgcgccgg cgccgcggac gctttggacg gcgccgccgg cctcgtcaac
     2101 gccagcgcgg tcggcatgct gccgagccgc gattcccctg tgccgcgcgc gttgttgcgc
     2161 cgcgaacttt gggtcgccga tgcggtctat cagccgctct ggacgccgct cctcgcggcg
     2221 gcgcgggaca aaggcgcggc gacgatgacc ggccgcgagc tggcggtcga tcaagccatc
     2281 gacgctttcg agattttcac cggccggaca gcgagccgcg cggtcttgga gcaggccttc
     2341 gatgaggtca tccggcgacg ggacgccgcg ttgacttgag ccgaggcgcc gcttcatttg
     2401 tcatatgtta agcggcggcc ttaaaaagcc gcgtcaggga ggaaggaaaa tgatcaagcg
     2461 ttcggcgtct ctcggcatcg cggtcctggc gagcttcctt gcggccggcg ccgcggctca
     2521 ggcggcggag acgatcaacc tcgccgacgt cgctgagctt tccggctccg gcgcgacggt
     2581 cggcaccaat tggaagaacg gcgtcgacct cgccgtcgaa gagatcaatg cccatggcgg
     2641 aatcctcggc gcgaaaatcg tcgtcaccca cgccgattcg caatcgaacg ccggcatcgc
     2701 caaggcgcag gtgcagaagg cgctggacgg ggagccctac gcgctgctcg gcccgggcta
     2761 ttccggctcg gtcaaggtct gcgagccgct ggcggcggaa gccggtatcg ccgagatcat
     2821 gggcggcgaa gccgccgagc tgacgcggat gggcaataaa tatgtcttcc gcacctcgtt
     2881 cggccagcaa tcgagcatgc cgaaaatcgc caaatacatt gccgagaacc tgaaggcgaa
     2941 gaccgcggca atcgtctatg tgaacaacga tttcggccgc ggcggccatg acatgatcct
     3001 gaaagaattc gccgcgcgcg gcgtcaaggt ggcgctcgac gattccaccg aggccggcca
     3061 ggctgacttc gccgccgacg cgctcaaggt gaagaacgcc aatccggacg tcgccttcat
     3121 ctatctcaac gaggaagaga gcgcgcgcat gctgaaggaa ttgcgcaagg ccgacgtcaa
     3181 ggcgccgctg gtcggcgaaa cgactctgat cgggcagaag gtgatcgagc tcgccggcga
     3241 cgccgccaat ggcgcgatcg gccatgtcgg cctcacgacc gacgcgccgg ttccggctct
     3301 caaggcttac ggcgaaaggt tctacgccaa ataccattac gtccccgacc ataacggcat
     3361 caaaggctac ctcgccgtct acatgatcaa ggcggcgacc gagaaaatgg gcaagctcga
     3421 ttcgaaaggc ctcgccgccg cgctgcacgg gctgacgatc accaccgcga tggagccgaa
     3481 cattctgatg gatgtatcca tcgatcagaa cggcgatctc gaccgtcaga gcttcatcgg
     3541 caaagtcatc gacgggaagc agacgatcac cgaagttctg ccgaagttga aggactgagc
     3601 cgacgcgatg aggtcgctcg gcccgagggg tcgcgcctac cgctcgcgcc gtcctcctcc
     3661 tgcgagcggg aaagggagcg cgccgcggtc atcggccgcg catccgccga aggcggcggg
     3721 gaaagcgccc ggaagggccg catgagaacg agccggactt gcaagtgaag cgggcgtcgg
     3781 cggcgagcag gggagcgtcg tggccaattt catcgcgcta ttgatcgcgg gtctcgccac
     3841 cggcgcgctc tattcgctgg tcgcgatcgg cttcactttg ctgtggcaga cctcgcagac
     3901 gatcaatttc gcgcagggcg aatttgtcat gttgccggcc tttttcatgt tggcggcgat
     3961 gaacgtccat ctgccgttct gggcggcgat tctcatcggc ttggcgctgt ccatggcgct
     4021 tct
//
LOCUS       DATGJW010000138         7812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1164218, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000138 DATGJW010000000
VERSION     DATGJW010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7812
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164218"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..97)
                     /locus_tag="VKU03_04185"
     CDS             complement(<1..97)
                     /locus_tag="VKU03_04185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HLJ70492.1"
                     /translation="MREGPSPIWRTVLLPRTTRRSLPMALLRAREA"
     gene            237..1847
                     /locus_tag="VKU03_04190"
     CDS             237..1847
                     /locus_tag="VKU03_04190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009363989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLJ70493.1"
                     /translation="MIERIADAFDYIVVGGGSSGCAAAARLSENPKNRVLLVEGGRRD
                     RSPWIHVPATFFKVLERGRDVLVYESEPEPALHGRRAIVPQGGVLGGGSSVNAMVYIR
                     GAAADYDSWAQMGNRAWSYERVLPVFRGLEDNARFSDEYHGVGGPLHVSDLADGHALS
                     KAFIAAAREAGLPANADFNGATQEGVGFYQTTTYRGRRWSAARAFLREAERRGNLAIL
                     TGRRCARIIFEGRRAAGVVLEDGAIYRAEGETVLCAGALETPRLLQLSGIGDAKALTE
                     LGVACIADLPGVGENYQDHFETPVQVEINEPTSLLGHDRGLRAVWHMAKYLIGRNGVL
                     ASNVVECGGFAKVAGADAPNIQFHVLPLVVGFVDRVPEPVHGVTVNPCLLRPRSRGSV
                     KIVSADPRVKARFRSGALTDPMDMHAQICGVELALKIVESPSLKRLVKRRILPKPGAE
                     TDREALAEHIRATSKTVFHPSGTCKMGPAEDRSAVVGQDLAVHGVAALRVADASIMPT
                     LVSGNTNAPAIMIGARCANFILGREAAA"
     gene            1902..2240
                     /locus_tag="VKU03_04195"
     CDS             1902..2240
                     /locus_tag="VKU03_04195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70494.1"
                     /translation="MSSRDIEWSLKEKKIARRAFDAALEETLAKTMAEFKRRANEAAT
                     PDEMWAVEDYLRQRRKEIDATFDYRYSQLLFVFARLIQDGSLDEARLAGLAEEKAKLI
                     RSYRRLAERD"
     gene            2427..4214
                     /locus_tag="VKU03_04200"
     CDS             2427..4214
                     /locus_tag="VKU03_04200"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ70495.1"
                     /translation="MIKAEREARIQAILEEVRVASVQEIAKRLGQASLVTVRRDIARL
                     AAQGLVRRSHGGAAAPQTRPPAPKAAPPRDSPANLDGVGAIVLPPLEGSGAETLRLMA
                     RRRRIPFLAESAPQEGGVYLGPDNHAAGCDLGRLAGAELNGRIKSAKILIVSLEALPN
                     TRLRSDAFLEGFRSAFKGEIRHWRVDGRGAFKSALRASLDALQAHPDINVLFGVNDHS
                     VLAALEASERLGHDRVHAFSVGGEGAALFEALSTGGKLRACAALFPEFVGARAVEVLG
                     QALAGGAMPREVKTPHAILTPSTLGKYYRRAEEGWAFASDSVPAGLAAAEALPKFSGP
                     RPTIGFIPHYPAHDWYRNMQRAMQRRADELGLALRVAAPETGIAREIQALRRVIARAA
                     AAMIKPGDTVLINQGETSLYLAEELRAAQNLTIVTNALDVVDRLNGRPGLKVILTGGE
                     LQTKDRCLVGPSLGGLFETIRVDKAFLSVDGVTARFGSSAGDERLALAGRRCAEASRE
                     VVVLADHSLIGQDASHRIVPPDGVDEIITDSGSLPSHRFALASTGARVTLADEEARES
                     SGQASGPPARSPSEPGRSDNLKPWEETYA"
     gene            4207..5415
                     /locus_tag="VKU03_04205"
     CDS             4207..5415
                     /locus_tag="VKU03_04205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184865.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ70496.1"
                     /translation="MRRAIALASILLTLPASAYAEDIVLKFWDNQQTESGLSQYQKEA
                     VKSFESANPGIKVEVTTIPYPEYQQRLLTAVQGGNAPDVSTIDQIWVAAFAEAGAIEP
                     LDDLAKKSGIAANAFFPGAWASANYGGKLWGVPFNVDVWQFSFYNADLLKAAGVEPQS
                     IATFDGLAAAGKKLTGNGKYGIGLFAHKGEDTVVVMDSFIFSNGGKVLNDDGSCGLTS
                     EASVGALKYLQSLAPYAPQGIANAASGDMRELFLNGSLAIEFWPALEQPTLQKSKINW
                     DFVDGTAPAGKKPIGTFGGWNLVMYKSSPHKDAVWKFIQFLTREDVNGKVVDLIPANI
                     KAADAFLKANRKGPEKILEQLNNAAPRPLSPRYLEVSNIEVTLAQDIYAGMDAAKAAA
                     KACKAIDATK"
     gene            5644..6528
                     /locus_tag="VKU03_04210"
     CDS             5644..6528
                     /locus_tag="VKU03_04210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLJ70497.1"
                     /translation="MPKRGAWLVWIFMTPAILLVGGLMYYPMIGTIVHSLYSTSFINP
                     HPQFVGLKLYAKILSSGEFGQIAWNSCVWTVFVVLFQNLFGFLTALLLNQRLPTTALM
                     RSLVLLPWVLPGVVGAILWRFMEDPQLGLINSFLMSLGLIDHGVAWLASTSTAMAAAI
                     AAAVWKGFPFSTVIYLAALQNVDQEQVESAIIDGASRLQRIIYVVLPSMREVIAINLV
                     LTTILTFNYFDMIWVLTRGGPENATHIFPTKIYELGFGEFRFGEAATYGVFSIIVLAL
                     LIATYFLIQRSGMRWRAR"
     gene            6525..7370
                     /locus_tag="VKU03_04215"
     CDS             6525..7370
                     /locus_tag="VKU03_04215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLJ70498.1"
                     /translation="MTARSWIGQVFLGKAGLTILQVALAAIILLPFFWMVSVSLKPAS
                     EPFAIPARLWPQNPTLDNYVTAFRPEFRTYFVNSLIVSLSTVAITVALALPAAYSFTR
                     AQTRLVFAIMGLIVAAQMFPASAIIIPIYKMMKGAGLLNTYASLIIAYVTITLPVAIW
                     MLRGFLARLPVELDEAAAIDGAGPFRTFVSIIVPLCRPGIVATAVFVLIVTWQEFLFA
                     LSFTSTRDMRTLPVGMNDFIGQYGIRYGELMASSVLISLPVIALFFLLQRQFVAGVTA
                     GAVKG"
     gene            7374..>7812
                     /locus_tag="VKU03_04220"
     CDS             7374..>7812
                     /locus_tag="VKU03_04220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLJ70499.1"
                     /translation="MPDSVLLSETIEPGIVRLTFNRPEKLNALSSEVIRRLDEALDRI
                     AADASARVVILTGAGERAFVAGADIGEYRGRRAKPFIAYQHESRRVFDKLEALPKPTI
                     AALRGYALGGGFEIALCCDVILCAASARLGLPEGLLGLSPGGGG"
BASE COUNT         1335 a         2541 c         2487 g         1449 t
ORIGIN      
        1 cggcttcacg ggcgcgcagc aacgccattg gaagggagcg gcgtgtcgtt cgcggcagca
       61 ggacggttct ccagatcggc gatgggcctt cgcgcaccct gccatcgctg gccgcataag
      121 acaacggacc ttcgttgaac gcgccgagcc gaatccccca gtcgttggac aagaatattt
      181 cacatgttaa attttggtcg cgacgatcgc atgggcgcgt ccgagggagg aaagggatga
      241 tcgagaggat cgcggacgct ttcgactaca ttgtcgtcgg cgggggatcg tccggttgcg
      301 ccgccgcggc gcgcctcagc gaaaatccga agaaccgggt gctgctggtc gaaggcggcc
      361 ggcgcgaccg cagcccatgg attcatgttc cggcgacgtt tttcaaagtg ctcgagcgcg
      421 ggcgcgacgt gctggtctac gaaagcgagc cggagccggc gcttcatggc cgcagggcga
      481 tcgtgccgca aggcggcgtc ctcggcggcg gaagctcggt caacgccatg gtctatatcc
      541 ggggcgcggc ggcggactat gattcctggg cgcaaatggg caaccgggcc tggtcttatg
      601 aacgggttct gccggtcttt cgcgggcttg aggacaatgc ccgtttcagc gacgagtatc
      661 acggcgtcgg cggacctctt catgtttccg atcttgccga cggccatgcg ctgtccaagg
      721 ccttcatcgc ggcggcgcgc gaggccggct tgccagccaa cgccgatttc aacggcgcga
      781 cgcaggaggg cgtcggattc taccagacga cgacctatcg cggccgccga tggagcgcgg
      841 cgagggcgtt ccttcgcgag gcggagcggc gcggaaatct cgccatcctg accggacggc
      901 gctgcgcgcg catcattttc gagggaaggc gcgccgccgg cgtcgtcctc gaagacgggg
      961 cgatctatcg ggcggaaggc gagacggtcc tctgcgccgg cgcgctggag acgccgcgcc
     1021 ttctgcagct ttcaggaatc ggcgacgcca aagctctaac cgagctcggc gtcgcctgca
     1081 tcgccgatct gcccggcgtc ggcgagaact atcaggatca tttcgaaacg ccggtacaag
     1141 tggaaattaa cgaaccgacc tcgctcctcg gccatgacag gggtctgcgg gccgtctggc
     1201 atatggcgaa gtacctgatt ggccgcaacg gcgtgctcgc ctcgaatgtc gtcgaatgcg
     1261 gcggcttcgc taaagtcgcc ggcgccgacg cgccgaacat ccagttccat gtcctgccgc
     1321 tggtcgtcgg ctttgtcgat cgcgttcccg agcctgtcca tggcgtcacc gtcaatccct
     1381 gccttctgcg gccccgctcg cgcggcagcg tcaagatcgt ttcagccgat ccgcgcgtca
     1441 aagcgcgctt ccgctccggc gcgctgaccg atccgatgga catgcacgcc cagatttgcg
     1501 gcgtcgaact ggcgctgaaa atcgtcgagt cgccgtcgct gaagcgcctc gtcaagcgcc
     1561 gcattctgcc gaagccgggc gcggagacgg atcgggaagc gctggccgag catatccgcg
     1621 cgacatcgaa gacggtgttc catccctcgg gcacgtgcaa gatggggccg gcggaggatc
     1681 gctccgccgt ggtcggccag gacctcgccg tgcatggcgt cgccgcgctg cgggtggcgg
     1741 acgcctcgat catgccgacg ctggtcagcg gcaacaccaa cgcgccggcg atcatgatcg
     1801 gcgcgcgctg cgccaacttc attctcggac gagaggcggc ggcgtaaagc ctccgtcgcg
     1861 cggaattgac cagcgtaccg ggctgctttt ggaggcgtgt catgagttcg cgcgatatcg
     1921 aatggtctct caaggaaaag aaaatcgcgc gtcgcgcatt cgatgcggcg ctggaggaga
     1981 cgctcgccaa aacaatggcc gagttcaagc gaagggccaa tgaagcagcg acgcccgacg
     2041 agatgtgggc ggtggaggat tacctgcgcc agcggcgcaa agaaatcgat gcgacgttcg
     2101 actaccgcta ttcgcagctc ttgttcgttt tcgcccgcct catccaggac ggcagcttgg
     2161 acgaagcgcg cctcgccggg ctggcggagg aaaaggcgaa gctcattcgc tcatatcgcc
     2221 gcctggcgga gcgcgattga ggtcggtttg agcacgacgc atcctgatgc agcgaggttc
     2281 tgccccgcgc tgtcatccga cgaggcgaag gacggatttc ctagaaccat aagtttagat
     2341 cgaatttgat cgtttctgga cgataattgc gcttgtctcc gtcaattcga gtatgttacc
     2401 cctcagtaga ccaattatga tccgaaatga tcaaagctga acgcgaggcc cgcatccagg
     2461 ccattttgga ggaggtgaga gtcgcctccg tccaggagat cgccaagcgc ctcggacagg
     2521 cgtcattggt gaccgtccgc cgcgacatcg cccgcctcgc cgcgcagggg cttgtgcgac
     2581 ggagccatgg cggcgccgcc gcgccgcaga ctcggccgcc agcgccgaaa gccgcgccgc
     2641 ctcgggattc tccggccaat ctggacggcg tcggcgcgat cgttctgccg ccgctcgaag
     2701 ggagcggcgc cgagacgctg cggctgatgg cgcgccggcg gcgcattccg ttccttgcgg
     2761 aatccgcgcc gcaggagggc ggcgtctatc tcgggccgga caatcacgcc gccggttgcg
     2821 atcttggccg gctcgccggc gccgaactca acggccgaat caaatcggcc aaaatcctca
     2881 tcgtctcgct ggaagcgctg cccaataccc ggctgagaag cgatgcgttt ctggagggct
     2941 tccgctccgc cttcaagggc gagatccggc attggcgcgt cgatggccgc ggcgccttca
     3001 aatcggcttt gcgcgccagc ctcgacgctt tgcaggcgca tcccgacatc aacgtccttt
     3061 tcggcgtcaa cgaccactcg gtcctcgccg ctttggaggc ctccgagcgg ctcggccacg
     3121 accgcgtcca cgctttttcg gtcggcggcg agggcgccgc gttattcgag gccctttcga
     3181 cgggcggcaa gctgcgcgcc tgcgcggctt tgtttcccga atttgtcggc gcgcgggcgg
     3241 tcgaggtcct cggccaggct ttggccggcg gcgcgatgcc gcgcgaggtg aagacgccgc
     3301 acgccattct cacgccgagc acgctcggca aatattaccg gcgcgccgag gagggatggg
     3361 ctttcgcctc ggatagcgtc ccggcgggct tggccgctgc ggaagcgctt ccgaaattca
     3421 gcggcccgcg gccgacaatc ggcttcattc cgcattatcc ggcccatgat tggtaccgca
     3481 atatgcagcg cgcgatgcag cggcgcgccg acgagctcgg gctcgcgctg cgcgtcgccg
     3541 cgccggagac cgggatcgcg cgcgaaatcc aggctttgcg ccgggtgatc gcccgcgccg
     3601 cggcggcgat gatcaagccg ggcgatacgg tgctgatcaa tcaaggcgag acatcgctct
     3661 atctcgcgga agaattgcgc gccgcgcaaa atcttacgat cgtcaccaac gcgctcgacg
     3721 tggtcgaccg cttgaatggc cggccgggcc tgaaggtcat tctgaccggc ggcgagttgc
     3781 agacgaagga ccgctgcctc gtcgggccga gcctcggcgg cctgtttgag acgatcaggg
     3841 tcgacaaggc cttcctctcg gtcgacggcg tcaccgcgcg tttcggctcc tccgcgggcg
     3901 acgagcgatt ggcgcttgcc gggcgacgct gcgccgaagc ctcgcgcgaa gtcgtcgtcc
     3961 tggctgatca ctcgctgatc ggccaggacg ccagccaccg catcgtgccg cccgacggcg
     4021 tcgacgagat catcaccgat tccggctcgc tgccgagcca tcgcttcgcg ctcgcctcga
     4081 ccggcgcgcg cgtcacgctc gccgacgaag aagcaagaga atcgagcggc caggcctccg
     4141 gcccgccggc gcgctcgccg tccgaaccgg gccgcagcga caatcttaaa ccgtgggagg
     4201 aaacctatgc gtagagcaat agcgctggcg tcgatcttgc tgacgctgcc cgcctcggct
     4261 tacgccgagg acatcgttct gaaattctgg gacaaccagc agaccgaatc cggcctgagc
     4321 caataccaga aggaggcggt gaagagcttc gagtccgcca acccggggat caaggtcgaa
     4381 gtgacgacca ttccgtaccc tgaatatcag cagcgcctgc tgaccgccgt acagggcggc
     4441 aacgccccgg acgtttcgac gatcgatcaa atctgggtcg cggccttcgc cgaagctggc
     4501 gcgatcgagc cgctcgacga tctcgccaag aaaagcggaa ttgccgccaa cgcctttttc
     4561 cctggcgcct gggcgtcggc gaattacggc ggcaagcttt ggggcgttcc gttcaatgtc
     4621 gatgtctggc agttcagctt ctacaatgcc gacttgctca aggcggccgg cgtcgagccg
     4681 caaagcatcg cgacctttga cggccttgca gccgcgggca agaagctcac cggcaacggc
     4741 aaatacggaa tcggcctgtt cgcccataag ggcgaagaca ccgtcgtcgt catggacagt
     4801 ttcatcttct cgaatggcgg caaggtgctc aatgacgacg gctcgtgcgg cctgaccagc
     4861 gaggcctcgg tcggcgcgct gaaatatctg caatcgctcg ccccctacgc gccgcaagga
     4921 atcgccaacg ccgcttccgg cgacatgcgg gaattgttcc tcaacggctc cctggcgatc
     4981 gaattctggc ctgcgctcga acagccgact ttgcagaagt cgaagatcaa ctgggatttc
     5041 gtcgacggca cggcgccggc gggcaaaaag ccgatcggca cgttcggcgg ctggaatctc
     5101 gtgatgtata aatcgtcgcc gcataaggac gcggtttgga aattcattca gttcctcacc
     5161 cgcgaggacg tcaacggcaa ggtcgtggat ttgattcccg ccaacatcaa ggccgccgac
     5221 gccttcctca aggccaaccg aaagggaccg gagaaaatcc tggagcagct caacaacgcc
     5281 gcgccgcggc cgctgtcgcc gcgctacctg gaggtctcga atatcgaggt cacgctggcg
     5341 caggacattt atgccggcat ggacgctgcg aaagcggcgg cgaaagcctg caaagcgatc
     5401 gacgcgacga agtgagcgag atattgaaag ccggctcgcc ttctcggggc tcccgggaag
     5461 gtcggcgccg cgaaaccggc gttcggccac ggccgaatgt cgccctcggc cgccaggtgt
     5521 gaccagtcga tgtggcggaa agcttccgtc tcccgcgcca gcgcgcagat cggcgatctc
     5581 gcgcttattc ggcgcgcttc gccggcgatc tcccgcgccg ggcgcgtaag gcggcaaaaa
     5641 gccatgccga aacgcggcgc ttggctggtc tggattttca tgaccccggc gatcttgctt
     5701 gtcggcgggt tgatgtacta tccgatgatc ggaacgatcg tccacagcct ctattcaacc
     5761 tccttcatca atccgcatcc gcaattcgtc ggcctgaagc tctacgccaa gattctctcc
     5821 agcggcgaat tcggacaaat tgcgtggaat tcctgcgtct ggaccgtctt cgtcgtcctg
     5881 ttccagaatc tcttcggatt cctcaccgcg ctgctgctca accagcgcct gccgacgacc
     5941 gccctgatgc gttcgctcgt cttgctgcct tgggtcctgc ccggcgtggt cggagccatc
     6001 ctctggcgct tcatggagga cccgcagctt gggctaatca actccttcct gatgagcctt
     6061 gggctgatcg accacggcgt cgcctggctg gcctcaacct cgacggctat ggccgcggcg
     6121 atcgccgcgg cggtgtggaa gggatttccc ttctcgaccg tcatctatct cgcggccttg
     6181 cagaacgtcg accaggagca ggtcgaatcc gcgatcatcg atggggcgag ccggctgcag
     6241 cgcatcatct atgttgtcct cccctccatg cgcgaggtca tcgcgatcaa cctcgtgctc
     6301 acaacgattc tcaccttcaa ctatttcgac atgatctggg tcttaacgcg cggcggaccg
     6361 gaaaacgcca cgcatatatt tccgacgaag atctacgagc tcggtttcgg cgagtttcgc
     6421 ttcggcgaag ccgccacata cggcgtgttc tcgatcatcg ttctggcgct gctcatcgcc
     6481 acatattttc tgatccagcg ctccggcatg cgatggaggg cgcgatgacg gcgcgatcct
     6541 ggatcggcca agttttcctc ggcaaagccg gattgaccat ccttcaggtg gcgctcgccg
     6601 cgatcatcct gctgccgttt ttctggatgg tttccgtctc gctgaagccg gcgagcgagc
     6661 ctttcgcgat tccggctcgg ctctggccgc agaatccgac tttggacaat tacgtgacgg
     6721 cgttccggcc ggagttccgg acctatttcg tgaactcgct gatcgtctcc ctttcgacgg
     6781 tggcgatcac cgtcgcgctc gccttgccgg cggcctatag tttcacccgc gcccagacgc
     6841 ggctggtttt cgccatcatg ggcctcatcg tcgcggcgca gatgtttcca gcgagcgcga
     6901 tcatcatccc catctataag atgatgaagg gcgcaggcct cctcaacacc tacgcgtccc
     6961 tgatcatcgc ctatgtcacc atcacgctgc cggtggcgat ctggatgctg cgcggctttc
     7021 tcgcgcgctt gccggtcgag ctcgacgagg ccgcggcgat cgacggggca gggccgttcc
     7081 ggacctttgt ctcgatcatc gtgccgctct gccggccggg cattgtcgcg accgccgtct
     7141 tcgtgctgat cgtgacttgg caggaattcc tcttcgcgct gtccttcacc tcgacccggg
     7201 acatgcggac gctgccggtc ggcatgaacg acttcatcgg ccaatacggc atccgctacg
     7261 gcgaattgat ggcgagttcg gtgctgatct cgctgcccgt catcgcgttg ttcttccttt
     7321 tgcagcgcca attcgtcgcc ggcgtcaccg ccggcgcggt caaaggatag gccatgccgg
     7381 attctgtgct gctgagcgag acgatcgagc cgggaattgt gcgactgacc ttcaaccggc
     7441 cggaaaagct caacgcgctt tcgagcgagg tgatccgccg tctcgacgaa gcgctcgacc
     7501 gcatcgccgc tgacgccagc gcgcgcgtcg tcatcctgac cggcgcgggc gagcgcgctt
     7561 tcgtcgccgg cgccgatatc ggcgaatatc gcggccggcg cgccaagccg ttcatcgcct
     7621 atcagcacga gagccgccgc gtcttcgaca agctcgaagc cttgccgaag ccgacgatcg
     7681 ccgcgctgcg tggctatgcg ctgggcggcg gcttcgagat cgcgctgtgc tgcgacgtca
     7741 tcctttgcgc ggcgagcgcg cggctcggcc tgccggaagg cctgctcggc ctttcgcccg
     7801 gcggcggcgg ca
//
LOCUS       DATGJW010000139         4111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_439322, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000139 DATGJW010000000
VERSION     DATGJW010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4111
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439322"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..751)
                     /locus_tag="VKU03_04225"
     CDS             complement(<1..751)
                     /locus_tag="VKU03_04225"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70500.1"
                     /translation="MQTGFGAFVSVYLTAERWSQTDIGLVLSVGAAIVLLGQIPGGAA
                     VDAASSPRRITALAVVAVGASAFALAASASAPIVFVARAVQAGASCVLTPAIAAISLG
                     LVGRSAISRRLGRNAAFASIGSAFAAAGMGVCAYWLSNRAAFLLVAGMTAPALLALSR
                     IAAGEIDPIRAHGGIARPDRRSVAAGLLALLEIRPLLIFAGCIVLFQLANAAMLPLAA
                     GLMTLRSSKAAAMLVAASILGPQLVVALLSPW"
     gene            complement(885..1907)
                     /gene="cobD"
                     /locus_tag="VKU03_04230"
     CDS             complement(885..1907)
                     /gene="cobD"
                     /locus_tag="VKU03_04230"
                     /EC_number="4.1.1.81"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011993127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0048472 - threonine-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine-phosphate decarboxylase CobD"
                     /protein_id="HLJ70501.1"
                     /translation="MNTIADPPLPAPRVDHGGDLAAARLAFPGAPEPWLDLSTGVNPN
                     PYPLPKLPPEAWARLPDRAGLARLEQVAARRYRAPAGAEIVAAPGAQALIQILPRLLR
                     AKTVGLLGFTYGEYEHVWRAAGAEIVVAETLDRLAACDLAIIVNPNNPDGRMVAPSDL
                     AALAHALARRGGALLVDEAFIDAAPAEASLVPALPMGAIALRSFGKIYGLAGLRLGFA
                     LASPKHAGKLRALLGPWAVSGPAIEIGAAALADEAWLAATAADLAARAAALDRLLIRH
                     GFSPLGGAPLFRLVAHPQADRIFRALGRQGVLARRFPTRPDWLRFGLPADGAGLARLE
                     ATLASA"
     gene            1906..2898
                     /gene="cbiB"
                     /locus_tag="VKU03_04235"
     CDS             1906..2898
                     /gene="cbiB"
                     /locus_tag="VKU03_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016880 - acid-ammonia (or amide) ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-phosphate synthase CbiB"
                     /protein_id="HLJ70502.1"
                     /translation="MSLSVTVPLALAALAAEALIGYPPALFKRLGHPVTWIGALISAT
                     EAALNRERFGAGARRALGAASLVLWLIAAGAISGAVVWACGGSLAGFAVLALVAASLP
                     AQRSLHDHVKAVAEALEGGGLAAGRQAVSLIVGRRTDALDEAGVARAAIESLAENFSD
                     GIVAPAFWLAVAGLSGGVLYKTINTADSMVGHRTPRYEAFGWAAARLDDLVNLPASRL
                     AALWLVLAALLDGPAAARAALGAVVRDAARHRSPNAGWPEAAMAGALGLQLAGPRVYG
                     ETLVEDAFMGDGRRAAKAADIRRALALYRRAAAIQFLALAAAAAALIARNWLAL"
     gene            complement(2939..4111)
                     /locus_tag="VKU03_04240"
                     /pseudo
     CDS             complement(2939..4111)
                     /locus_tag="VKU03_04240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013636996.1"
                     /note="frameshifted; incomplete; missing N-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=2
                     /transl_table=11
                     /product="cobyric acid synthase"
BASE COUNT          663 a         1406 c         1478 g          564 t
ORIGIN      
        1 cccaaggcga gagcagcgcg acgacaagtt gcggcccgag gatcgaggcg gcgaccaaca
       61 tcgcggcggc cttgcttgag cgcagcgtca tgaggcccgc cgccaaaggc agcatggcgg
      121 cgttggcgag ctggaacaga acgatgcagc cggcgaagat cagcagcggg cggatttcga
      181 gcaaagcgag caggccagcc gcgacgctcc tccggtccgg cctggcgatc ccgccatgcg
      241 ccctgatcgg atcgatctcg cccgcggcga tgcggctcaa ggcgagcagg gccggcgccg
      301 tcatgccggc gacgagaaga aaagcggccc gattggaaag ccaataggcg caaacgccca
      361 tgccggcggc ggcgaaagca gagccgatcg aggcgaaggc cgcgttgcgg ccgaggcgcc
      421 gactgatcgc cgatcggccg accaggccga ggctgatcgc ggcgatggcg ggggtcaaga
      481 cgcaactcgc cccggcctga accgctcgcg cgacgaagac gatcggcgcg ctcgccgaag
      541 cggcgagcgc gaaagcgctg gcgccgacgg cgaccacggc caaagccgtt atccgccgcg
      601 gcgaggacgc cgcatccacg gcggcgccgc caggaatctg gccgagcaag acgatcgccg
      661 cgccgaccga caagacgagt ccgatatcgg tctggctcca tctttcggcg gtcagataga
      721 ccgagacaaa ggcgccgaac ccggtttgca cgtcggccag gaaaaagacg aacccgtcga
      781 gcgcgtaaag gctgactctg gcaggagcag gggagcgaat ccttctatcc tccgcgcgcc
      841 aacgcgccgc cggcgcggcg cgttccctcc aactccgccg gcgctcaggc cgaagccagc
      901 gtcgcttcga ggcgcgccaa accggctccg tcggccggca ggccgaagcg cagccaatcg
      961 ggccgtgtcg ggaagcgccg cgccaagacg ccctggcggc caagcgcgcg aaagatgcgg
     1021 tcggcttggg gatgcgccac gaggcggaag agcggcgcgc cgccgagcgg cgagaagcca
     1081 tgcctgatca ggagacgatc gagggccgcg gcgcgggcgg caaggtcggc ggccgtcgcc
     1141 gcgagccatg cctcgtcggc gagcgccgcg gcgccgattt cgatcgccgg gccggaaacc
     1201 gcccaggggc cgagcagcgc gcgcagcttt ccggcgtgct tcggagatgc gagtgcaaaa
     1261 cccagccgca ggccagcaag gccgtagatt tttccgaagg agcgcagcgc gatcgcgccc
     1321 atcggcaatg ccgggaccaa gctcgcctcg gctggggccg catcgataaa ggcttcatcg
     1381 acgaggagcg cgccgccgcg ccgcgccagg gcatgggcga gagcggccaa gtcgctcggc
     1441 gcgaccatcc ggccgtcggg gttgttcgga ttgacgatga tcgcgaggtc gcaggcggcc
     1501 agccgatcga gcgtttcggc gacgacgatt tcggcgcccg ccgcccgcca gacatgctcg
     1561 tactcgccat aggtgaagcc caacaggccg accgttttgg cgcgcaacag ccgcggcaag
     1621 atttggatga gcgcctgcgc gcccggcgca gcgacaattt cagcgccggc cggcgcgcga
     1681 tagcgcctgg cggcgacctg ctccagacgg gcaagcccgg ctcggtcggg cagccgggcc
     1741 caagcttccg gcggcagctt cggcaaggga taaggattgg ggttgacgcc ggtcgaaaga
     1801 tcaagccagg gctcgggcgc gcccggaaag gccaggcgcg ccgcggcgag atcgccgccg
     1861 tgatcgaccc ttggggcggg aagaggcgga tcggcaatcg tgttcatgtc gctatccgtc
     1921 accgttcccc tggccctcgc cgcgctggcg gctgaggctt tgatcggtta tccgccggcg
     1981 cttttcaaac ggcttggcca tcccgtgacc tggattggcg ccctgattag cgcgaccgag
     2041 gcggcattga acagggagcg cttcggcgcc ggcgcgcggc gcgcgctcgg cgcagcgagc
     2101 ctcgtcctgt ggctgatcgc cgccggcgca atttccggcg cggtcgtctg ggcttgcggc
     2161 ggctcgctgg ccggcttcgc cgtcttggcg ctggtcgccg ccagcctgcc ggcgcagcgc
     2221 agtctgcatg atcacgtgaa ggcggtcgcc gaggccttgg agggcggcgg cctggcggcg
     2281 ggccgccagg ctgtgtcgct gatcgtcggc cgccgcaccg atgcgctcga cgaagccggc
     2341 gtcgcgcgcg ccgccatcga gagcctggcg gaaaatttct ccgacggaat cgtcgcgccg
     2401 gccttctggc tggcggttgc cgggctgtcc ggcggcgttc tctacaagac gatcaacacc
     2461 gccgacagca tggtcggcca tcggacgccg cgttacgaag ccttcggctg ggccgcggcg
     2521 aggctggacg atctcgtcaa tctgccggcc tcgcgccttg cggcgctgtg gctcgtctta
     2581 gcggcgctgc tcgatggacc ggccgcggcc cgcgccgcgc tcggcgcggt tgttcgcgac
     2641 gccgcgcgcc accgctcgcc gaacgccggc tggccggaag cggcgatggc cggcgcgctc
     2701 ggcctgcaac tcgccggtcc gcgggtctat ggcgagactt tggtcgagga cgccttcatg
     2761 ggcgacggcc gacgcgcggc gaaggcggcc gacatccgcc gcgcgctcgc cctctatcgg
     2821 cgcgccgcgg cgattcagtt cctcgcgctg gcggccgccg ccgcggctct catcgcgcgg
     2881 aactggttag cgctgtagcc gcgctcgccg accgcggcta cagcgccgac agcttggctc
     2941 atttggcgag ggcgagcagc cgatcgagat cgagatggcg ctcgagatgg gcggcgagat
     3001 cgtcgagcac gcgttcgatc gtcgcctcgt agcgaagcgc cgagccggcc ccgccgagcc
     3061 gttcgagcca ggcccggcgc tgggcgtcgt cggcgaacag gccatggaca taggcgccgg
     3121 cgacgaggcc gctcgccgac atggcgcctt cctttcgccc gtcgtcatga acgagcaagg
     3181 ggcgagcgcg atccggccct tcggtgcggc cgatatgcat ctcgtagccg gaaaaagggg
     3241 cgccagtcag cgccgagcgt ccacagacat ggacgagcgt cttatcgccg atcagcacgg
     3301 tctccacatc gagcaggcca aggccaggca gttcctcggc cggcccttcg atcccttcgg
     3361 gatcggcgat cgtgcggccg agcatttggt agccgccgca caggccgagc acgaagccgc
     3421 cgcgccggac atgcgcctta tgtcgatgtc ccatcccgcc gcgatcagcg ccttgagatc
     3481 ggccagcgtc gccttcgagc cgggcaggat gacgagagca gcgtcggcgg ggatcggcgc
     3541 gtcgagccgg atcatcgcca agctcacatc cggctcctgg gcgagcggat cgagatcgtc
     3601 gaaattggcg atatgcggca ggaccggtac gacgattttg atccttccgc cgcctggcgg
     3661 gcgcgcccgt tcgagcgcga cgctgtcctc ggccggcaac agacgggcgg cggcaaaata
     3721 aggtacgagc ccgagcgccg gccagcccga cagccgctcg accgccgcca tgccgtcggc
     3781 gaaaagcgac gcgtcgccgc ggaacttatt gacgatgaag cccttgacca ttgcggcgtc
     3841 ggcttcgtcc agagcgactt tcgtgccggc gatctgggcg atgacgccgc cgcggtcgat
     3901 gtcgccgatc agcgcgaccg gacaatcggc ggcgcgggcg aagcccatat tggcgatgtc
     3961 gccggcgcgc aggttgattt ccgaggcgcc gcccgcccct tcgacaagaa cgagatcggc
     4021 gtcggcggat agaatctcga agctctgcag cacggcgttt atcaacttgg gcttcatcgc
     4081 ttggtattcg cgcgccttgg ccgcgccgac g
//
LOCUS       DATGJW010000140         6732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1164408, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000140 DATGJW010000000
VERSION     DATGJW010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6732
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1164408"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VKU03_04245"
     CDS             <1..870
                     /locus_tag="VKU03_04245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage T C-terminal barrel
                     domain-containing protein"
                     /protein_id="HLJ70503.1"
                     /translation="YTQLLNETGGVLDDLMIVGRPPAKAGEERLFLIVNAARKGDDFA
                     HIRRLLSDLRLRELGDRALLALQGPRAAAILAKRWPKLAAVPFMTAVSAEELGDAGSD
                     YLFSRSGYTGEDGFEISLPADEAAAFARGLLAEADVKPIGLGARDSLRLEAGLCLYGH
                     DIDETTSPVEAGLSWSIPKRRRAEGGFPGAERIVREIADGPARRRVGLRLDGKAPARE
                     GAEIVNADGATIGRVTSGGFGPSVGAPIAMGYVDAAWSGPGSAVDLVVRGKKLAARVA
                     PMPFHPHAYFRGA"
     gene            890..1267
                     /gene="gcvH"
                     /locus_tag="VKU03_04250"
     CDS             890..1267
                     /gene="gcvH"
                     /locus_tag="VKU03_04250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0019464 - glycine decarboxylation via
                     glycine cleavage system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycine cleavage system protein GcvH"
                     /protein_id="HLJ70504.1"
                     /translation="MSQLRYSKDHEYIRLEGEIGTVGISDHAQAQLGDVVFVELPQVG
                     AQLVQGGEAAVVESVKAASEVYAPATGEVVAVNDALEGQPSLINEDATGKGWLFRVKL
                     ADPKELESMMDEAAYQEFLKTEA"
     gene            1272..2615
                     /gene="gcvPA"
                     /locus_tag="VKU03_04255"
     CDS             1272..2615
                     /gene="gcvPA"
                     /locus_tag="VKU03_04255"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963099.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPA"
                     /protein_id="HLJ70505.1"
                     /translation="MRYLPLSDEDRALMLARIGVADIDALFADIPASKRLTGLIDIPR
                     RMSEMEVERRLSAMAARNVAAGSAPFFLGAGAYRHHVPASVDHLIQRSEFLTSYTPYQ
                     PEIAQGTLQTLFEFQTQVAALTGMEVANASMYDGSTACAEAVLMAHRLTKRKKAVLSG
                     GLHPHYAAVVRTLATMAEDETVILPPDVFAREDIAAAIDEETSCVVVQNPDFFGNLRD
                     LAPIAAAAKAKGALLVAVFTEAVSLGAVRSPGEMGADIVAGEGQSIGNALNFGGPYVG
                     LFATRLKNVRQMPGRLAGETVDAEGRRSFTLTLSTREQHIRREKATSNICTNSGLCAL
                     AFTIHMSLLGEKGLRSLAKVNHANAVALAERLAGVSSVSILNECFFNEFTLRTPRPAS
                     EVVDALAKKGVIGGVPVSRLLPKAGLDDLIVVASTETDSESDRAAYAAALAGILS"
     gene            2653..4200
                     /gene="gcvPB"
                     /locus_tag="VKU03_04260"
     CDS             2653..4200
                     /gene="gcvPB"
                     /locus_tag="VKU03_04260"
                     /EC_number="1.4.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004375 - glycine dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006546 - glycine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminomethyl-transferring glycine dehydrogenase
                     subunit GcvPB"
                     /protein_id="HLJ70506.1"
                     /translation="MLNRQGRATRAVEVALTAPATFTGNRGLDMEEALIFETGRLDAT
                     GVDIDEPAPFASRLGGHERVAPIGLPGLTEPECLRHYVRLSRKNYSIDAGLYPLGSCT
                     MKHNPRLNEKMARLAGFADIHPLQPFSTAQGALELIDALAKTLMALTGMSAVAMSPKA
                     GAHGELCGMMAIKAAILARGEAETRRVVLVPDSAHGTNPATAALIGFSVRAVPANAEG
                     IVTAQAVKAAIGPDVAAIMLTNPNTCGLFEREIAEIADTVHRAGAYFYCDGANFNAIV
                     GKAKPGDLGVDAMHINLHKTFSTPHGGGGPGAGPVVLSERLAPFAPVPFLKRDGDALT
                     LVEHAEGGQSFGRLSAFHGQMGMFVRALAFILSHGADGLKQASEDAVLSANYIRVCLN
                     DLMTSPFGDRPCMHEALFDDRWLKETGITTLDFAKAMIDEGYHPMTVYFPLVVHGAML
                     IEPTESESKASLDLFIMTLRDLALGALAGDRDRFLSAPRFAPRERLDETRAARQPILT
                     WTKPTPA"
     gene            4320..5048
                     /locus_tag="VKU03_04265"
     CDS             4320..5048
                     /locus_tag="VKU03_04265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HLJ70507.1"
                     /translation="MPALLCAIVIAAFAAGDAAKAQSQPATPAAQTPAAAPAPSAAPP
                     APAPATPAVPPAAAGASPNAPAAPATPQAQTPAAPLPAPPQSDAAETPTIQLAARPAA
                     YIEGKANWDEGFSAINNAIAAVDAELAKAGMRPAGRPLAVFTETDDTSFRFRAMVPID
                     AIPDGKTSLSDTVKFGQTPAGKAMKFEHRGSYEDIDSTYEAITAFLDEKGLESQNLFY
                     EEYLNDVKSPDDPTLEVDIYVLLK"
     gene            5045..5743
                     /locus_tag="VKU03_04270"
     CDS             5045..5743
                     /locus_tag="VKU03_04270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016848208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAD hydrolase-like protein"
                     /protein_id="HLJ70508.1"
                     /translation="MSAAQSSRPPPSVLFDLDGTLTDSGPGIVNCALYALRRFNETRG
                     AALPIPEPRALRFLLGPPLRESFVKLAGPEAADALLAFYRERYESFGLLENSVFPNIA
                     SSLEALRAKGSRLLVATSKPEHYARRILDHFDLSRFFAAIHGAQPDGTRANKGALIAY
                     VLDRYAIDPARAAMIGDREHDILGAKANGVRGVGALWGYGSREELQAAGADPIVSRPG
                     DLAAALNAVFSGAA"
     gene            5857..6084
                     /locus_tag="VKU03_04275"
     CDS             5857..6084
                     /locus_tag="VKU03_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CopG family transcriptional regulator"
                     /protein_id="HLJ70509.1"
                     /translation="MRTTMTIADDVLVAAKAIAREQDKTVGEVISDLARRALRKPSGR
                     RERNGVPLLPVQKGHPVVTLEIVNALRDEAS"
     gene            6081..6518
                     /locus_tag="VKU03_04280"
     CDS             6081..6518
                     /locus_tag="VKU03_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TA system VapC family ribonuclease toxin"
                     /protein_id="HLJ70510.1"
                     /translation="MTFLLDVNVLIALIDPTHVSHDAAHLWFHREGRRSWATCPITEN
                     GVIRIVGSSKYPNTPGSPAVVAEIVRQLREAPGHVFWADSASLLDSKRVDISEVLTAK
                     QVTDSYLLALAVEHGGGLATFDRRLSTKAVNGGKSALHLIVAD"
     gene            complement(6535..>6732)
                     /locus_tag="VKU03_04285"
     CDS             complement(6535..>6732)
                     /locus_tag="VKU03_04285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70511.1"
                     /translation="PVEAVEVLKLCGARHAIAHHWGTFPLSNEAIDAPPKALAEALHK
                     AAVASERFRVMRPGEVYEEAP"
BASE COUNT         1137 a         2303 c         2153 g         1139 t
ORIGIN      
        1 tatacccaat tgctcaacga gaccggcggc gttctcgacg acctgatgat cgtcggccgg
       61 ccgccggcca aggccggcga agagcgcctc ttcctgatcg tcaacgcggc gcgcaaaggc
      121 gacgatttcg cgcatatccg ccggctcttg tcggacctgc ggctgcgcga gctcggcgac
      181 cgcgccttgc tcgccttgca gggcccgcga gccgccgcga tcctcgccaa gcgctggccg
      241 aaactggccg ccgtcccgtt catgacggct gtttcggccg aggagctcgg cgacgccggc
      301 agcgattacc tcttctcgcg ctctggctat accggcgagg acggtttcga gatttcgctt
      361 ccagcggacg aagcggcagc cttcgcccgc ggcttgctcg ccgaggcgga cgtcaagccg
      421 atcggtctcg gcgcgcgcga ttcgctgcgc ctcgaagccg gcctttgcct ctatggccat
      481 gacatcgacg agacgacgag cccggtcgag gcggggcttt catggtcgat cccgaaacgc
      541 cggcgggccg agggcggctt tcccggcgcc gagcgcattg tgcgcgaaat cgccgatggg
      601 ccggcgcgcc gccgagtcgg cttgcggctc gatggcaagg cgccggcgcg cgaaggcgct
      661 gaaatcgtca acgccgacgg cgcgacgatc ggccgcgtca cgtcgggagg cttcggcccg
      721 agcgtcggcg cgccgatcgc gatgggctat gtcgacgcgg cgtggagcgg cccgggctcg
      781 gccgtcgatc tcgtcgtgcg cggcaagaag ctcgccgccc gcgtcgcccc catgccgttc
      841 catccgcacg cctatttccg cggcgcgtga cgcccgaaag gagaaatcca tgtcgcagct
      901 tcgctacagc aaggaccacg aatatatccg ccttgaaggc gagatcggca cggtcggcat
      961 cagcgaccac gcccaggccc agctcggcga cgtcgtgttc gtcgaattgc cgcaggtcgg
     1021 cgcccagctc gtccagggcg gcgaggcggc ggtcgtggaa agcgtcaaag cggcgagcga
     1081 ggtctacgcg ccggcgaccg gcgaagtcgt cgccgtcaac gacgcgctcg aaggccagcc
     1141 cagcctgatc aacgaggacg cgaccggcaa aggctggctc tttcgcgtca agctggccga
     1201 tcccaaggag ctcgaatcca tgatggacga agccgcgtac caagaattcc tcaagacgga
     1261 agcgtgatcc gatgcgctat ctgcccctca gcgacgaaga ccgcgcgctg atgctggcgc
     1321 ggatcggcgt cgccgatatc gacgcgcttt tcgccgatat tcccgccagc aagcgcttga
     1381 caggcctcat cgacattccg cggcggatga gcgagatgga ggtcgagcgc cggctttcgg
     1441 cgatggccgc ccgcaacgtc gcggccggct cggcgccgtt ctttctcggc gccggcgcct
     1501 atcgccacca tgtgccggcg agcgtcgatc atctcatcca gcgctccgaa tttctgacca
     1561 gctacacgcc ctatcagccg gaaatcgcac aagggacgtt gcagaccttg ttcgagttcc
     1621 agacccaggt cgcggcgctg accggcatgg aggtcgccaa cgcctcgatg tatgacggct
     1681 cgaccgcctg cgccgaagcg gtgctgatgg cgcaccgtct gaccaagcgc aaaaaggcgg
     1741 tgctgtccgg cggcctgcac ccgcactacg ccgcagtcgt ccgcaccttg gcgacgatgg
     1801 ccgaggacga aaccgtcatc ctgccgccgg acgttttcgc gcgcgaggat atcgccgcgg
     1861 cgatcgacga ggagacgagc tgcgtcgtcg tgcagaatcc cgatttcttc ggcaatttgc
     1921 gcgaccttgc gccgatcgcg gccgccgcta aagccaaggg cgccctcctc gtcgcggtct
     1981 ttaccgaggc ggtctcgctc ggcgcggtgc gctcgcccgg cgagatgggc gccgacatcg
     2041 tcgccggcga aggccaatcg atcggcaacg ccctgaattt cggcggtccc tatgtcggcc
     2101 tgttcgcgac gcgcttgaag aacgtccgcc agatgccggg gcggctcgcc ggcgagacgg
     2161 tggatgcgga agggcggcgc agtttcaccc tgacgctctc gacccgcgag cagcatatcc
     2221 ggcgcgagaa ggcgacgagc aacatctgca ccaattccgg gctctgcgcc ctcgccttca
     2281 ccatccatat gagcctgctt ggcgaaaaag gcttgcgcag tctcgccaag gtcaaccacg
     2341 ccaacgccgt cgcgctggcg gagaggctcg ccggcgttag cagcgtcagc atcctcaatg
     2401 aatgcttctt caacgagttc acgcttcgca cgccgcgccc ggcttccgag gtcgtcgacg
     2461 cgctggcgaa gaagggcgtg atcggcggcg ttccggtctc gcgcctcctg cccaaggccg
     2521 gactcgacga cctcatcgtc gtcgccagca ccgagaccga tagcgagagc gaccgcgccg
     2581 cctacgccgc agcgctcgcc ggaattctgt cgtgacggat caaggcgcga gaggctccaa
     2641 ggaccgatga acatgctgaa caggcaagga cgcgcgaccc gcgccgtcga ggtcgcccta
     2701 acggcgcccg ccactttcac cggcaatcgc ggcctcgaca tggaggaggc gctgattttc
     2761 gagaccggca ggctcgatgc gaccggcgtc gacatcgacg agccggcgcc tttcgcctcg
     2821 cggctcggcg ggcatgagcg cgtcgcgccg atcggcctgc ccggcctgac cgagccggaa
     2881 tgcctgcgcc attatgtgcg cctgtcgcgc aagaactatt cgatcgacgc cggcttgtac
     2941 ccgctcggct cgtgcacgat gaagcacaat ccgcgcctca acgagaaaat ggcgcggctc
     3001 gccggcttcg ccgacatcca tccattgcaa cccttctcga ccgcgcaagg cgcgctcgaa
     3061 ctcatcgacg cgctggcgaa gactctgatg gcgctcaccg gcatgagcgc ggtcgcgatg
     3121 tcgcccaagg ccggcgcgca tggcgaactc tgcggcatga tggcgatcaa ggccgcgatc
     3181 ctcgcgcgag gagaggccga gactcggcgc gtcgtgctgg tgcccgattc ggcccatggc
     3241 accaatccgg cgaccgccgc cctgatcggc ttttcggtgc gcgccgtgcc ggccaatgcc
     3301 gaaggcatcg tcacggcgca ggccgttaaa gcggcgatcg gaccggacgt ggcggcgatc
     3361 atgctgacca atcccaatac gtgcggcctg ttcgagcggg aaatcgccga gatcgccgat
     3421 accgtccacc gagccggcgc ttatttctat tgtgacggcg ccaatttcaa cgcgatcgtc
     3481 ggcaaggcca agccgggcga tctcggcgtc gacgcgatgc atatcaacct gcacaagact
     3541 ttttcaacgc cgcatggcgg cggcggcccg ggcgccggcc cggtcgtgct gtcggagcgc
     3601 ctggcgccgt ttgcgccggt tccgttcctc aagcgcgacg gcgacgcgct gaccctggtc
     3661 gagcatgccg aaggcggaca atctttcggc cggctgtcgg cctttcacgg ccagatgggc
     3721 atgttcgtgc gcgccctcgc cttcatcctc tcgcacgggg cggacgggct gaaacaagcg
     3781 tcggaagacg cggtcctatc ggccaattac atccgcgtct gcctcaacga cctgatgacg
     3841 tcgcccttcg gcgatcgtcc ctgcatgcac gaggccttgt tcgatgaccg ctggctgaag
     3901 gagaccggga tcacgacgct cgacttcgcc aaggcgatga tcgacgaggg ctatcatccg
     3961 atgacggttt attttccgct cgtcgtccat ggcgccatgc tgatcgagcc gaccgaatcg
     4021 gaatccaagg cgagcctcga tctgttcatc atgacgctgc gcgatcttgc gcttggcgcc
     4081 ctcgccggcg accgtgaccg cttcctgtcg gcgccgcgtt tcgcgccgcg cgaacggctc
     4141 gacgagactc gggcggcgcg gcagcccatc ttgacatgga ccaagccgac gccggcctag
     4201 ccgcttgctt ggcgccgcgc gagactcgat tcatactggc gtgagggacc gatttgttcg
     4261 gcgcctcgcg gtcagagaaa cgaacaatgt tcggcaaacc cgtagcttcg ttaagacttt
     4321 tgcctgcctt gctttgcgcg atcgtcatcg ccgcgttcgc ggcgggcgac gcggccaagg
     4381 cccagagcca acccgccacc ccggcggcgc aaacgcccgc tgcggcgccc gccccttccg
     4441 ccgcgccgcc ggcgcccgcc cccgcgacgc cggccgtccc gcctgcggcc gccggcgctt
     4501 cgcccaacgc gcccgccgcg ccggcgacgc cgcaagccca gacgcccgcg gcgccgctcc
     4561 cggccccgcc gcaaagcgac gccgccgaga cgccgaccat ccagcttgcc gcccgcccgg
     4621 ccgcctatat cgagggcaag gcgaattggg acgaaggatt cagcgcgatc aacaacgcca
     4681 tcgccgcggt cgacgccgaa ctcgccaaag ccggcatgag gccggcgggg cgcccgctcg
     4741 ccgtcttcac cgagaccgac gatacgagct tccgcttccg cgccatggtg ccgatcgacg
     4801 ccatcccgga cggcaagacg agcctctccg atacggtgaa attcggccag acgccggccg
     4861 gcaaggcgat gaaattcgag catcgcggct cgtatgaaga catcgattcg acctatgagg
     4921 cgatcaccgc ctttctcgac gaaaaggggc tcgaatcgca aaatcttttc tacgaagagt
     4981 atttgaacga cgtgaaatcg ccggacgatc cgacattgga agtcgatatt tacgtgctgc
     5041 tcaagtgagc gccgcgcaaa gctcgcgccc gccgccgtcg gtcctgttcg atctcgatgg
     5101 aacgctgacc gattcgggac cgggcatcgt caattgcgcg ctctacgcct tgcgccgctt
     5161 caacgagacg cgcggcgcgg ctctgccgat ccccgagcct cgcgccttgc gtttcctgct
     5221 ggggccgcct ttacgggaat ccttcgtcaa gctggccggc cccgaagcgg ccgatgcgct
     5281 gctggcgttt tatcgggagc gctacgagtc cttcggcctt ttggagaaca gcgtcttccc
     5341 gaacatcgcg tcgagcctgg aggcgctccg cgcaaagggg agtcgtctct tggttgcgac
     5401 atcgaagccg gagcattacg cgcgtcgcat tctcgatcat ttcgatcttt cgcgcttctt
     5461 cgccgccatc catggcgcgc agcctgacgg gacgcgcgcc aataaaggcg cgctgatcgc
     5521 ttatgtgctg gaccgatacg cgatcgaccc ggcgcgcgcc gcgatgatcg gcgaccgcga
     5581 gcacgacatt ttgggcgcga aggcgaacgg cgtacgcggc gttggcgcgc tatggggcta
     5641 tggctcgcgc gaggaattgc aggccgccgg cgcggatccg atcgtctcgc ggccagggga
     5701 tcttgccgcg gcgctgaacg cagttttttc cggcgcagcc tgaccgagtc cgtccgtttg
     5761 ggaagcgcgc ctgcggaaga cggcccttcc ctcttgccgc gcaattgcct atatttgatg
     5821 cgcgatgcat gatgtttgat gcaggatagt cgcccgatgc ggacaacaat gacgattgca
     5881 gacgatgttc tggtcgcggc caaggcgatt gcacgggagc aggacaaaac cgtcggggag
     5941 gtgatctccg atctcgctcg gcgcgccctc cgcaaaccgt cggggcggcg cgaacgcaat
     6001 ggagttcccc ttctgccggt tcagaaaggc catccggtcg tcacgctgga gatcgtcaac
     6061 gcgctgcgcg atgaagcgtc gtgacgtttc tgcttgacgt caatgtgctg atcgcgctga
     6121 tcgacccgac ccatgtaagc catgacgccg ctcacctctg gtttcatcgc gaaggtcgtc
     6181 gcagttgggc tacctgcccg atcacggaga acggcgttat ccgtatcgtc ggaagctcga
     6241 agtatcctaa tacgccaggc tcgcctgcgg tcgtcgccga aatcgtccgt cagcttcgag
     6301 aagcgccggg tcacgtcttc tgggctgaca gcgccagcct cttggactcg aagcgggttg
     6361 atatatcgga agttcttact gctaaacagg tcaccgacag ctatcttctg gccctcgcag
     6421 tcgaacacgg cggaggactc gcgacattcg atcgccgact gtcgaccaag gccgttaacg
     6481 gcgggaaaag cgcgcttcat ctgatcgtcg ccgattgatg gcggcgccgc gccctcacgg
     6541 cgcctcctca taaacctccc ccggccgcat gacgcggaag cgctccgacg cgaccgccgc
     6601 tttgtgcaag gcctcggcaa gagccttggg cggcgcgtcg atcgcttcgt tgctcagggg
     6661 gaacgttccc caatgatggg cgatcgcgtg gcgcgcgccg caaagcttga ggacctcgac
     6721 cgcttcgacc gg
//
LOCUS       DATGJW010000141         9767 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_751384, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000141 DATGJW010000000
VERSION     DATGJW010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9767)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9767)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9767
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_751384"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1082
                     /locus_tag="VKU03_04290"
     CDS             114..1082
                     /locus_tag="VKU03_04290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70512.1"
                     /translation="MNAAGQDETGSRREDEGGLPDSRLRRDMPLVPASSIAGRALVFV
                     IAIMTFLSCLTAGGAMLVAGAAEGWRSDISREVTIQIKPQAGRDVEAAVEKAAAAARA
                     APGVDAVQVFSKADSERLLEPWLGAGLDLSELPIPRLIVVKMQKDADPDIGGLRQALA
                     SQAPEASLDDHRLWLSRLDTMASGVVIFALALFVLMIIAMMLAIGFATRGAMAGNREI
                     IEVLHFVGAADSYISRQFQGHFLRLGLRGGLLGGAAAALFFLSATGLSAWSAHSPGGE
                     EIVALFGAFALGAKGYIVIFALAIAIGALTAIMSRIIVFRHLRRLL"
     gene            1128..1886
                     /locus_tag="VKU03_04295"
     CDS             1128..1886
                     /locus_tag="VKU03_04295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLJ70513.1"
                     /translation="MIGLRSFAYNVAFYATLVFLMIVGLPSILMGRRAVFFMGRLWGA
                     CSLWLLDHICGLRVEFRGLENIPKGGYIIAAKHQSFLETFSLIEHAPDFAYVLKHELM
                     YIPVFGWYLWGAEQIGIDRARGKTALQVLSERAAAVLKAGRQIFIFPEGTRRPPGAPP
                     QYKSGVAYLYETSGAPCLPVALNTGLYWGRRGFRRRPGVAVIEYLSPIPPGLDREAFL
                     ARLQETIETACARLNAEAVAQDSSLALKLAPAQS"
     gene            2005..2418
                     /locus_tag="VKU03_04300"
     CDS             2005..2418
                     /locus_tag="VKU03_04300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70514.1"
                     /translation="MQALRDLRERGGGFAAAGETASAANSELAALAGETLSGRFRHWR
                     GASGRRYVFSVYDPDACPAYDDAVLIVATIGPSGERRIVSIADTGALPEIAVAHARRI
                     ASSLSMRVECHVHLLAASRFEREAAIADLRPFRPA"
     gene            complement(2415..3665)
                     /locus_tag="VKU03_04305"
     CDS             complement(2415..3665)
                     /locus_tag="VKU03_04305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008593850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70515.1"
                     /translation="MSAQSEQKPAQIFYGWFVVAAAFAVTFVGFGCAYAFGAFVQALR
                     TDFAASLGSVSAVFSIAAFFYFGLGIVSGPLADRWGARRLAVLGMVLTGLGLAAAALA
                     RSLAEVYAAYGIGVGLGVGCSYVPAVGAVQRWFSRRRGLASGLAVSGIGVGTLVMPPL
                     ASALISAFGWRGAYLILGAFAAIAGAGMALLIEDDPRRRGLAPDGDQLAPDAGAAKPA
                     GASVGQAVRSRAFVSLYAACLIASFGVFVPFVHLVPYAVSHGVAHSSAVLLVGMIGVG
                     STAGRFFLGGFADRLGRRAALLAMFLGMALALGFWGLATSLWPLAIFAFVYGVFYGGW
                     VAVLPAVVMDCFGGRAISGIIGALYTSVAFGTLIGPTAAGFAFDASHSYALPIAASAL
                     ANLAAAAIAATLLRKEKKALAPAR"
     gene            complement(3756..4322)
                     /locus_tag="VKU03_04310"
     CDS             complement(3756..4322)
                     /locus_tag="VKU03_04310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamylcyclotransferase"
                     /protein_id="HLJ70516.1"
                     /translation="MRVTRQDKSVDSDGDLWVFGYGSLMWRPGFSFIERQNGLVRGWR
                     RRLCVYSWVHRGTKERPGLVLGLDRGGACHGAAFRVAAKDREATIAYLREREQVTAVY
                     LEKHVRVLLWEGATVTALTYVADRRHPQYAGALEKQELLRLTATAKGRSGPNAEYILN
                     TVAHLEEVGIKDPTLEWLAEQLRALRTR"
     gene            complement(4510..5469)
                     /locus_tag="VKU03_04315"
     CDS             complement(4510..5469)
                     /locus_tag="VKU03_04315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate/arogenate dehydrogenase family
                     protein"
                     /protein_id="HLJ70517.1"
                     /translation="MAELGPALAEPLVQRLALIGLGLIGSSIARAARRMNAAATIVAA
                     DADPDVRARVAALNIADEIAPDLASAARRADLVILCAPVGANGAIADEIAPHLKKGAI
                     VSDVGSVKSAVVAALQPRLPAYVHLVPAHPVAGTEYSGPDSGFATLFRRRWCIITPPE
                     NSDEAAVTRVKALWEAFGSHVEIMSAAHHDLVLAMTSHIPHLIAYNIVGTAADLEEVT
                     SAEVIKFSAGGFRDFTRIAASDPTMWRDVFLNNKDAVLEVLGRFTEDLAALQRMIRWG
                     EGDKLFDLFTRTRAIRRGIIEQGQETAAPDFGRQAAENGGGQR"
     gene            complement(5462..6577)
                     /gene="hisC"
                     /locus_tag="VKU03_04320"
     CDS             complement(5462..6577)
                     /gene="hisC"
                     /locus_tag="VKU03_04320"
                     /EC_number="2.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004400 - histidinol-phosphate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphate transaminase"
                     /protein_id="HLJ70518.1"
                     /translation="MSANAPSRPTPRANVMAIEAYVPGKSAAPGAAKVFKLSSNETPL
                     GPSPAAIAAFRANAEHLAIYPDGSASALREAIGKRYGLDPARIVCGNGSDDLLGLIAH
                     AFLGSGDEGLYTQHGFLEYPIVTRAAGATPVVAAEKDYTADVDALLAKLSERTKVVFL
                     ANPNNPTGTYLPFAEVKRLHAGLPPHVLLVIDAAYAEYVARNDYAAGIELAAESENVV
                     MTRTFSKIYGLANLRLGWAYGPAHVIDALNRIRGPFNVNGAAIAAGIAALADQAHVDK
                     AVAHNERWLPWLATEVERLGIKATPSVGNFLLLHFPRQDGRDAKAADAFLTARGYILR
                     AVGAYGLPDCLRLTIGTEEANRGLVDALAEFMGKPRG"
     gene            complement(6828..7670)
                     /locus_tag="VKU03_04325"
     CDS             complement(6828..7670)
                     /locus_tag="VKU03_04325"
                     /EC_number="5.4.99.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0046417 - chorismate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HLJ70519.1"
                     /translation="MLKPSPNETLADLRVDIDRIDKAMHELLIERGEIIDRLIAIKAR
                     QGGGSAFRPAREAAMMRALAERHRGLLPLDTVEGVWRVIISTFTYVQAPYSVHCDMSR
                     GDAAMRDSARFHFGFTVPCVSHHSAAGVIQAVAAAKGDLGMFALDGGAAAGAWWTRLT
                     PSAAPKIIARLPLVERPDHPAGMPVFVIAKPLADGAARDVVIEAAALDRWRGDYPEAL
                     AEIGADIIGNAADGMGLSLLIARPGAVAAEALAEVLRKAGARDLRLTEVGAHAARFDF
                     AAKR"
     gene            complement(7802..8194)
                     /locus_tag="VKU03_04330"
     CDS             complement(7802..8194)
                     /locus_tag="VKU03_04330"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ70520.1"
                     /translation="MVILDTNVISELLAPKPNSSVEGWLAAQPIASVFTTAITKAEIL
                     RGVRFLPEGRRRRDLETAIRPIFTTEFEGRVLPFDAEAAEAYADLTAARRTVGRPISQ
                     SDAEIAAIALSRGSAVATRTWTISPILA"
     gene            complement(8194..8439)
                     /locus_tag="VKU03_04335"
     CDS             complement(8194..8439)
                     /locus_tag="VKU03_04335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012760767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HLJ70521.1"
                     /translation="MPSMTIRNLDEQLKKRLRVRAASHGRSMEDEARDILRAALSTEA
                     HRPRNLAEAIRARIAPLGGVKLEIAPREPMPPPVTFE"
     gene            8594..9142
                     /locus_tag="VKU03_04340"
     CDS             8594..9142
                     /locus_tag="VKU03_04340"
                     /EC_number="2.4.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003999 - adenine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenine phosphoribosyltransferase"
                     /protein_id="HLJ70522.1"
                     /translation="MSSPAHEIDLRKAIRSIPDYPKPGIVFRDITTLLGDARAFRRTV
                     DELAQPWAGMKIDKIAGIEARGFIIGGAVAHQVSAGFVPIRKKGKLPHTTVRVAYSLE
                     YGLDEMEMHEDAVKPGERVVLVDDLIATGGTAEGAVKLLRNMGAEVLAACFIIDLPAL
                     GGADKLRKLDVPVRTLIAFEGH"
     gene            complement(9166..>9767)
                     /locus_tag="VKU03_04345"
     CDS             complement(9166..>9767)
                     /locus_tag="VKU03_04345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018243511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLJ70523.1"
                     /translation="QEDFGEALLPAVLGRFARAHPRVRIEARIVRNAELIERVKSGRL
                     DLALAWSDGPALPHSERVADIPMRWVGPAGGAGWRPDSGEPLPLAALEAPCLFRTAAT
                     EALDRAGRPWRVAFESPSLGGLWAATAAGLGLTLRTAIGLPANLRPLAPGEAGLPPAP
                     MLGLALHRAEASPGPAVTRLAEIILQELREAIPQPRRAA"
BASE COUNT         1620 a         3261 c         3312 g         1574 t
ORIGIN      
        1 ggcaccgcgg tggtcatcgc cacccatgac atcgggttga tggaccaatt cgccgacgct
       61 cgccgtctcg tcatcagcgg cggccggctg gaaatcttcg gcagggattc ctggtgaacg
      121 cggcggggca agacgaaacc ggctcgcgcc gcgaggacga aggcggattg ccggattccc
      181 gcctcaggcg cgacatgccc ctggtgccgg cgagttcgat cgccggccgc gctttggtct
      241 ttgtcatcgc gatcatgacc ttcctctcct gcctgaccgc cggcggcgcg atgctggtcg
      301 ccggcgccgc ggaaggctgg cgctccgaca tttcccgcga ggtgacgatc cagatcaagc
      361 cgcaagccgg ccgcgatgtc gaggcggcgg tcgaaaaggc ggcggcggcg gcccgcgccg
      421 cgcccggcgt cgacgccgtg caagtcttct ccaaggccga ttccgagcgt cttttggagc
      481 cgtggctggg cgccggcctc gatctcagcg aattgccgat tccgcgactg atcgtcgtca
      541 agatgcagaa agacgccgac ccggatattg gcggcttgcg tcaagcgctg gcgagccagg
      601 cgcccgaggc gagcctcgac gatcatcgcc tgtggctgtc gcggctcgac acgatggcgt
      661 cgggcgtcgt catcttcgcg ctcgctttgt tcgtcctgat gatcattgca atgatgttgg
      721 cgatcggctt cgccacgcgc ggcgcgatgg ccggcaatcg cgaaatcatc gaggtgctgc
      781 atttcgtcgg cgccgcggac agctacattt cgcgccaatt ccaggggcat ttcctgcggc
      841 tcggcttgcg cggcggcctg ctcggcggcg cggcggcggc cttgttcttc ttaagcgcga
      901 ccggcctttc ggcctggtcg gcccattcgc ccggcggcga ggagatcgtc gcgctgttcg
      961 gcgccttcgc gctgggcgcc aagggctata tcgtcatctt cgccttggcg atcgcgatcg
     1021 gcgcgctgac cgcgatcatg tcgcggatca tcgtgttccg ccatttgcgg cggcttttgt
     1081 gaggcctcat ctttctcggc tgttcctttc aaggcatatt ttggctcatg atcggcctgc
     1141 gctccttcgc ctacaacgtc gctttctacg ccacgctcgt ctttttgatg atcgtcggcc
     1201 tgccctcgat cctgatgggc cgacgcgcgg tcttcttcat gggccgcttg tggggcgcct
     1261 gctcgctgtg gctgctcgat catatttgcg gcttaagggt cgaatttcgc ggcctcgaaa
     1321 acatcccgaa aggcggctac atcatcgccg ccaagcacca atcctttctc gagaccttct
     1381 cgctcatcga acacgcgccg gatttcgcct atgtgctcaa gcacgagctg atgtacatcc
     1441 cggtcttcgg ctggtacctt tggggggcgg agcagatcgg catcgaccgc gcccgcggca
     1501 agacggcgct gcaggttctc agcgagcgcg ccgccgcggt gctcaaggcc gggcggcaga
     1561 ttttcatctt tccggaagga acgcgccggc cgcctggcgc gccgccgcaa tacaaatccg
     1621 gcgtcgccta tctctacgag acgagcggcg cgccctgcct gcccgtcgcg ctcaataccg
     1681 ggctctattg gggccggcgc ggcttccggc gccgcccggg cgtcgccgtg atcgaatatc
     1741 tgtcgccgat cccgccgggc ctggaccgcg aggcttttct cgccaggctg caggagacga
     1801 tcgagaccgc ctgcgcccgg ctcaacgccg aagcggtggc gcaggattcc tccctcgcct
     1861 tgaagctggc gccggcgcaa agctgacatc ttttctccgc gttttcgccc ggtcgcgccc
     1921 gtactcggcg cgaatcggtt gcaaagcgtt gacgcgtcgc cgtagatgtt ctagatttgt
     1981 tcccttccac atcgggtttc ggccatgcaa gctttgcggg atttgcgcga gcgcggcggc
     2041 ggcttcgccg ctgcgggcga aacggcgagc gccgccaata gcgagctcgc cgctttggca
     2101 ggcgagactc tctccggacg cttccgccat tggcgcggcg cctccggacg gcgctatgtg
     2161 ttctccgtct acgatcccga cgcctgcccg gcttatgacg acgccgtgct gatcgtcgcg
     2221 acgatcggcc cgagcggcga acggcggatc gtctcgatcg ccgataccgg cgccttgccg
     2281 gagatcgccg tcgctcacgc gagacggatc gcctcatcgc tcagcatgcg cgtcgaatgc
     2341 cacgtccatc tcctcgccgc ctcgcgcttc gagcgcgaag ccgcgatcgc cgatctccgc
     2401 ccgtttcggc ctgcttagcg ggcgggcgcc aaagcttttt tctctttgcg cagcaaggtc
     2461 gccgcgattg cggcggcggc gaggttggcg agcgcgcttg cggcgattgg cagcgcgtag
     2521 gaatggctgg cgtcgaaagc aaagccggcg gcggtcggcc cgatcagcgt gccgaaggcg
     2581 acgctggtat agagcgcgcc gataatgccg ctgatcgcgc ggccgccgaa acaatccatg
     2641 accaccgccg gcaagaccgc gacccagccg ccatagaaca cgccgtagac gaaggcgaag
     2701 atcgcaagcg gccataggct cgtcgccagt ccccagaagc caagcgcaag cgccatgccg
     2761 agaaacatcg ccagaagcgc cgcgcgccgc cccagccggt cggcgaagcc gccgaggaag
     2821 aagcggccag ccgtgctgcc gacgccgatc atgccgacaa gcaaaaccgc cgacgaatgc
     2881 gcgacgccat ggctgacggc gtaagggacg agatgcacga atgggacgaa gacgccgaac
     2941 gaggcgatca ggcaggccgc gtagaggctg acgaaagcgc gcgagcgcac cgcttggccg
     3001 acagaagcgc cggcgggctt cgctgcgccg gcgtcaggcg cgagctgatc gccgtccggc
     3061 gccaagccgc ggcgtcgcgg atcgtcttca atcagcagcg ccatgccggc gccggcaatc
     3121 gccgcgaagg cgccgaggat gagataggcg ccgcgccagc cgaaggcgga gatcaaagcc
     3181 gaggcgagcg gcggcatgac cagcgtgccg acgccgatgc cgctgaccgc gaggcccgaa
     3241 gccaagccgc ggcgtcgcga aaaccagcgt tgcaccgccc cgaccgccgg gacataggag
     3301 cagccgacgc cgaggccgac gccgatcccg taagcggcgt agacttcggc caggctgcgc
     3361 gccagcgcgg cggcggcgag ccccagcccg gtcagcacca tgccgagcac ggcgaggcgg
     3421 cgcgcgcccc agcggtcggc gagcggaccg ctgacgattc cgaggccgaa atagaaaaag
     3481 gcggcgatgg agaagacggc cgacaccgag ccgagcgacg ccgcgaaatc cgtccgcagc
     3541 gcctgaacga aagcgccgaa cgcgtaggcg cagccgaagc cgacgaaggt gacggcgaag
     3601 gccgcggcga ccacgaacca gccgtagaag atttgcgccg gcttttgctc ggactgcgcg
     3661 ctcatcgact gtcgccggct tggcgatgcg ccttgtgttt cagtctcgcc aatcgctttc
     3721 gcatgatcca tcctccggcg gcaggcgggc cttgctcagc gcgtccgcag cgcgcgcaat
     3781 tgctccgcca gccattcgag agtcggatcc ttgatcccga cctcttcgag atgcgccacg
     3841 gtgttgagaa tatattcggc gttggggcct gaacgcccct tggctgtggc ggtcaggcgc
     3901 agcaattcct gtttttccag cgcgccggca tattgcggat ggcggcgatc ggcgacgtac
     3961 gttagagcgg tgaccgtcgc gccctcccag agcagcacgc gcacgtgttt ttcgagatag
     4021 acggcggtga cctgctcgcg ctcgcgcaga taggcaatcg tcgcttcgcg atccttggcg
     4081 gcgacccgga acgccgcgcc gtggcaggcg ccgccgcgat caaggccgag gaccaggcca
     4141 ggcctctcct tcgtgccgcg atggacccag gaatagacgc aaagacggcg ccgccagccg
     4201 cgcaccagcc cgttctgccg ctcgatgaac gaaaagcccg gccgccacat cagcgagccg
     4261 tagccgaaca cccagaggtc gccatccgag tcgacgctct tgtcctggcg agtcaccctc
     4321 atccggccct tcgggccacc ttctcccgca ggcgggagaa gggattccca accgctgtgc
     4381 gccgtctttt aaggacctgc cgcaggcggc cgtgacgcgc ccgacgcgcc ccctctcccg
     4441 cttgcgggag agggttgggg tgagggtacg ccgcggcgcc aacacgccgc gacaatcctg
     4501 tccgcaccat catcgctggc cgccgccgtt ttcggccgcc tggcggccga aatcgggggc
     4561 ggcggtctcc tggccttgct cgatgatgcc gcggcggatg gcgcgcgtgc gcgtgaagag
     4621 atcgaacaat ttgtcgccct cgccccagcg gatcatccgc tgcaaggcgg cgaggtcctc
     4681 ggtgaaacgg ccgagcactt cgagcactgc gtccttgttg ttgaggaaca cgtcgcgcca
     4741 catggtcgga tcggaggcgg cgatgcgggt gaaatcgcga aagccgccgg ccgagaactt
     4801 gatcacttcg gcgctcgtca cctcttcgag atcggcggcc gtgccgacga tgttataggc
     4861 gatcagatgg ggaatatggc tcgtcatggc gaggacgaga tcgtgatgcg ccgcgctcat
     4921 gatctcgaca tgcgagccga acgcctccca taaagctttg acgcgtgtga ccgccgcttc
     4981 gtcgctattt tccggcggcg tgatgatgca ccagcgccgc cggaacagag tcgcaaagcc
     5041 ggaatcggga ccggaatatt ccgtgccggc gacgggatgg gccgggacga gatggacata
     5101 agccggcagg cgcggctgca aggcggcgac caccgccgac ttgaccgagc cgacatcgga
     5161 gacgatcgcg cccttcttca gatgcggcgc gatctcgtcg gcaatcgcgc cgttggcgcc
     5221 gaccggcgcg caaagaatga ccagatcggc gcggcgcgcg gccgaggcga gatccggcgc
     5281 gatctcgtcg gcgatgttca gcgcggcgac gcgcgcgcgg acgtccggat cggcgtcggc
     5341 ggcgacgatc gtcgccgcgg cgttcatccg ccgcgccgcg cgggcgatcg acgagccgat
     5401 cagcccgagg ccgatcagcg cgaggcgctg gacgagcggc tcggcgaggg ctgggccaag
     5461 ctcagccacg cggcttcccc atgaactcgg cgagcgcgtc gacgaggccg cgattggcct
     5521 cttcggttcc gatcgtcagg cgcaggcaat cgggcaggcc ataggcgccg acggcgcgca
     5581 ggatgtagcc gcgcgcggtg aggaaagcgt cggccgcttt cgcgtcacga ccgtcttgcc
     5641 gcggaaaatg cagcagcagg aaattgccga cgctcggcgt cgccttgatc ccgagccgct
     5701 caacctcggt cgctagccaa ggcagccagc gctcgttatg cgccaccgct ttgtcgacat
     5761 gcgcctggtc ggcgagcgcg gcgattcccg ccgcgatcgc cgcgccattg acgttgaacg
     5821 ggccgcggat gcggttgagc gcgtcgatca catgcgccgg cccataggcc cagccgaggc
     5881 gcagattggc gaggccgtag attttcgaga aggtgcgcgt catcacgaca ttctcgctct
     5941 cggcggcgag ctcgatgccg gcggcgtagt cgttgcgcgc gacatattcg gcataggcgg
     6001 cgtcgatgac gagcaggaca tgcggcggaa gaccggcatg cagccgcttg acctcggcga
     6061 agggcaggta cgtgccggtc gggttgttcg gattggcgag aaacacgact ttcgtccgct
     6121 cgctgagctt ggcgaggagc gcgtcgacat cggccgtgta gtccttttcc gccgccacga
     6181 ccggcgtcgc gccggcggcg cgggtgacga tcggatattc gagaaagccg tgctgggtat
     6241 agagcccctc gtcgccagag ccgagaaagg cgtgggcgat caggccgagc agatcgtcgg
     6301 agccgttgcc gcagacgatc cgcgccgggt cgagcccgta acgcttgccg atcgcctcgc
     6361 gcaaggcgct ggccgagccg tcgggataga tggcgagatg ctcggcattg gcgcggaacg
     6421 cggcgatggc ggccggcgac gggccgagcg gcgtctcgtt agaggagagc ttgaaaacct
     6481 tggcggcgcc gggcgccgcg ctttttcccg gcacataggc ctcgatcgcc atgacattgg
     6541 cgcggggcgt cggccgggag ggggcgtttg cggacatgga aggaacgaac tccaaaagga
     6601 ctagagccaa gcaggcgaca tgggattgac gttaccgata gctccgccgc gcctcaccct
     6661 catccggcgc ttcgcgccac cttctcccgc aagcgggaga agggattgtc ggcgatcgcg
     6721 gctacaccgc aatcgcaatg acgcgcgctg tacgccccct ctcccgcttg cgggagaggg
     6781 ccggggtgag ggtaagccgc cgtctctgtc acttatgaaa atccaactta gcgtttggcc
     6841 gcgaaatcga accgcgccgc atgggcgccg acttcggtga ggcgaagatc gcgcgcgccg
     6901 gctttgcgca ggacctcggc cagcgcctcc gccgcgacgg cccccggccg cgcgatcagc
     6961 agcgacaggc ccatcccgtc ggcggcgttg ccgataatgt ccgcgccgat ctcggccaaa
     7021 gcctccggat agtcgccgcg ccagcggtcg agcgcggcgg cttcgatgac cacgtcgcgc
     7081 gcggcgccgt cggcaagcgg cttggcgatg acgaaaaccg gcatgccggc cggatggtcc
     7141 ggccgctcga cgagaggcag gcgggcgatg attttcggcg ccgccgacgg cgtcagccgc
     7201 gtccaccagg ccccggcggc cgcgccgccg tcgagcgcga acatgccgag atcgcccttg
     7261 gccgccgcga ccgcctggat gacgccggcg gcgctgtgat gcgacacgca aggcaccgtg
     7321 aagccgaaat ggaaacgcgc cgaatcgcgc atcgcagcgt cgccgcgcga catgtcgcaa
     7381 tggaccgaat agggcgcctg gacataggtg aaggtggaga tgatcacccg ccagacgccc
     7441 tcgaccgtat cgagcggcaa aagcccgcga tggcgctcgg cgagggcgcg catcatggcg
     7501 gcctcccgcg ccggccggaa ggccgagccg ccgccctggc gcgccttgat cgcgatcagc
     7561 cggtcgatga tctcgccgcg ctcgatcagg agctcgtgca tcgccttgtc gatgcggtcg
     7621 atgtcgacgc gcagatcggc gagggtctcg ttgggcgatg gcttgagcaa agcggcggct
     7681 ccacatgtgc cgggcgcttc ttacaggcgc cgaggcgcgg cgccaatcat tgccgccccg
     7741 gcggcgtttg ccggtccgaa gctttaggct cgcttagccg aaccccacgg attgatgatg
     7801 gttaggccaa tatgggagaa atcgtccacg ttcttgtggc gaccgccgag ccgcgagaaa
     7861 gcgcgatggc ggcgatttca gcatctgatt ggctgattgg tctgcctacc gttcgtcggg
     7921 cagcggtgag atcggcgtaa gcctccgcag cttcggcatc gaacggtaag actcgaccct
     7981 cgaattccgt ggtgaagatt ggccgaatcg cagtttcgag atcgcgccgc cgtcgccctt
     8041 caggcaaaaa tcgaacccct cgaagaatct ccgccttggt tatcgctgtt gtgaagaccg
     8101 atgcgatcgg ctgcgccgca agccaccctt cgaccgaact attcggcttc ggggctagca
     8161 attccgaaat aacattggtg tcgaggatga ccattattcg aatgtcacgg gcggcggcat
     8221 tggctcgcga ggcgcgattt caagtttaac tccgcccaag ggggcgatgc gcgctcgaat
     8281 cgcctcagcg agatttctcg gccgatgcgc ctcggttgac aaagccgccc gaagaatgtc
     8341 gcgcgcctcg tcttccattg agcggccatg ggaagccgcc cttacacgaa ggcgtttctt
     8401 cagttgttcg tccagattgc ggatggtcat gctgggcaaa gggaccccca ttcagcaatg
     8461 attgcataat agtcattgat gaccacctcg caagttcaac tgatccttaa acgcgggtgt
     8521 ctggaaggcg gcaagcttga ccctggcttt gcgggctgcg agccttagcc gatcccgcta
     8581 acgagcatcc ttcatgtcca gcccggctca cgaaatcgat ctgcgcaaag ccatccgcag
     8641 cattcccgat tatccgaagc ccggcatcgt cttccgcgac atcaccaccc tgctcggcga
     8701 cgcccgcgcc ttccgccgca cggtcgacga attggcgcaa ccctgggcgg ggatgaagat
     8761 cgacaagatc gccggcatcg aggcccgcgg cttcatcatc ggcggggcgg tggcgcatca
     8821 ggtctcggcc ggcttcgtgc cgatccgcaa gaaggggaag ctgccgcata cgacggtgcg
     8881 cgtcgcctat tcgctcgaat acgggctcga cgagatggag atgcacgaag acgcggtgaa
     8941 gcccggcgag cgggtcgtgc tggtcgacga cctcatcgcg accggcggca ccgctgaagg
     9001 ggcggtcaaa ttgctgcgca atatgggcgc cgaagtgctg gccgcctgct tcatcatcga
     9061 cctgccggcg ctcggcggcg cggacaagct gcgcaagctc gacgtgccgg tgcggacgct
     9121 gatcgccttc gaagggcatt aacggcgcgg ggcgtccgcc cggcgtcaag ccgcccggcg
     9181 cggctgcggg atcgcctcgc gcagctcctg caggatgatc tcggcgagcc gcgtcacggc
     9241 cggcccgggc gaggcctcgg cgcgatgcag cgcgaggccc agcatgggcg ccggcggaag
     9301 gccggcctcg cccggcgcga gcggacgcag attcgccggc aggccgatcg cggtccggag
     9361 ggtgaggccg agcccggcgg ctgtcgcggc ccagagtccg ccaagactcg gactctcgaa
     9421 ggcgacgcgc cacggccggc cggcgcgatc cagcgcctcg gtcgcggcgg tccggaacag
     9481 gcatggcgct tcgagcgccg ccagcggcag cggctcgccc gaatcgggcc gccaccccgc
     9541 gccgccggcc gggccgaccc agcgcatcgg gatgtctgcg acgcgctcgc tatgggggag
     9601 cgccggcccg tcgctccagg cgagcgccaa atcgagccgg cccgatttga cccgctcgat
     9661 gagctcggca ttgcggacga tgcgcgcctc gatcctcacc ctcggatggg cgcgggcgaa
     9721 gcggccgagc acggcgggca gcagcgcctc gccgaaatcc tcctgca
//
LOCUS       DATGJW010000142         5200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_439950, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000142 DATGJW010000000
VERSION     DATGJW010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5200
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_439950"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            419..1117
                     /locus_tag="VKU03_04350"
     CDS             419..1117
                     /locus_tag="VKU03_04350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HLJ70524.1"
                     /translation="METWVFDLDNTLYPPDSDLWPMIDQRISLFVMDMFGLDGLSARA
                     LQKYYYHRYGTTLRGLIEEHAVGPDEFLAFVHEIDRSSLAPNPPLAKEIAALPGRKLI
                     LTNGSRDHALRTAAQLGLSALFEDAFDIVAANMVPKPAPEAYDAFFKKYGVEPARAAM
                     FEDIARNLIVPHERGMTTILVRPRADQIDYREAQDRRDEDSPAVDFATSELAGFLQRV
                     NLELRLADESPRPA"
     gene            complement(1132..1956)
                     /locus_tag="VKU03_04355"
     CDS             complement(1132..1956)
                     /locus_tag="VKU03_04355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HLJ70525.1"
                     /translation="MTPPVDFDELKLRLIEIQPELPKRLQQVAAFALEHPDEMALGTA
                     ASVAGHARVQASTLVRFAQSIGFSGFSDLQEIFRSRLRNRWPDYPARMMALHDKAMDS
                     GDPTHLLAGFADSATASLARLRESVTREAFDRAIETLADARTIYLLGQRRAFPVAHYL
                     AYALAKLGVGAALIDNVGSLGAEQLAHAGRDDALIAISFAPYTPFTVELTRHAAARGV
                     PVVVITDSPLSPLASLAKARFEVAESDFGAFRSLSATFCLAITLAVALANRRSESG"
     gene            2140..3249
                     /locus_tag="VKU03_04360"
     CDS             2140..3249
                     /locus_tag="VKU03_04360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003587607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ70526.1"
                     /translation="MRAIGVGLIGTGYMGKCHALAWTGVKAVFGDTPDIRLASLCEVT
                     PELAAKKAAEFGFAKPTADWRALIADPETDVISVTTPNQFHADMAIAALEAGKHVWCE
                     KPMAPNYADAERMAAAARRSGRAAILGYNYIQNPAIRYIRKLLDEGRIGAVNHLRIEM
                     DEDFLADPDQPHSWRSEASAGYGALDDFAVHPLSLISTLFGRAEAVFGEMSKPYPDRP
                     HQGGRRAVETYDIATALLRLPGGISGAIHVNRCAWGRKGRIFVQIFGGRGMIAFDQER
                     FNEVQVYVAEGRPEDQGFRTILSGPAHKPYDRFIVAPGHQLGFNDLKIIECRALIGRM
                     LGEKTVAIDFDEGLAIERAVHAIARASREGRWVQV"
     gene            3234..4328
                     /locus_tag="VKU03_04365"
     CDS             3234..4328
                     /locus_tag="VKU03_04365"
                     /EC_number="2.3.1.78"
                     /inference="COORDINATES: protein motif:HMM:NF019403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015019 - heparan-alpha-glucosaminide
                     N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0007041 - lysosomal transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparan-alpha-glucosaminide N-acetyltransferase"
                     /protein_id="HLJ70527.1"
                     /translation="MGTGVREAAAFLLREAGEGDHAEHGGEGLVASGQSPTPRPPPPR
                     SARSPSHASRGRIAAIDAARGAALIGMAAYHFSWDLAYFGLAAPNFPLTPPMRLFSHC
                     VAGAFLALVGASLVLAHHDGFHRRAFQRRLALICGAAALVTIATAILAPQDAIYFGIL
                     HCIAVASLLAAPLLQTRLWAPFAAAGFAFLAPVLFAGPAFNQPAVIWLGLNTAPPSTF
                     DWRPLAPWSGLVFLGLAFARLWWRPLTESRLATWRPRAVAARALAWAGRRSLPIYLIH
                     QPVLFALLFAATGFASPDARRDEVNFRNVCLAQCGRAGGTADHCQRGCQCVVGGLRDA
                     GLAIAFSRAHLDDAQHKRFSSIVQACARAP"
     gene            complement(4482..>5200)
                     /gene="iolB"
                     /locus_tag="VKU03_04370"
     CDS             complement(4482..>5200)
                     /gene="iolB"
                     /locus_tag="VKU03_04370"
                     /EC_number="5.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862590.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016861 - intramolecular oxidoreductase
                     activity, interconverting aldoses and ketoses [Evidence
                     IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5-deoxy-glucuronate isomerase"
                     /protein_id="HLJ70528.1"
                     /translation="TYVGFEARDLKQGARHAGETGKTEACIVLLSGKARVTAKNFDSG
                     VVGERANVFDGLPWSVYLPAGATYAVEAATDCEIALCTAPATGKLPARVIGPAEVETT
                     TRGAGSNTRFVRNILSETVPAESLLVVEVITPGGNWSSYPPHKHDRDALPEESYLEET
                     YYHRIAPKQGFAFQRVYTDDRSLDETLTVSDRDVVLVPRGYHPVGAPHGYELYYLNVM
                     AGPKRAWRFYNDPDHAWMLK"
BASE COUNT          838 a         1723 c         1714 g          925 t
ORIGIN      
        1 tccggccagg ggcaccaagg cgccggttgc ggcgcctgcg ctatgcgcgc tctcccgatt
       61 cttgcccgcc gaagcatagg cggccgcgct tttgcaacgt tacgccgttc gtttattcgt
      121 tccggcgtcg ccgtcggagc cgcgctcgcg cggccccatt gtggccaaac caagtttgta
      181 aattcggtca gtctgttgca agattggtcg atttcgaatg tcgagtcgtt gtcgttgaac
      241 gatcggccgt tcgggatcga ggctccggcg gctgaggcgg gcgtcgtcgg cgtcccgctc
      301 gcaaacctga aactcgattc gctaaccgtc ggcatcgttc ttgaacaagc ccttttccca
      361 tttcaccacg gccgccggcg gcgacgagac agccgcgctt ctcgcgaaat tccgtcatat
      421 cgagacctgg gtcttcgatc tcgacaacac gctctatccg cctgatagcg atctctggcc
      481 gatgatcgac cagcgcattt cactgttcgt gatggacatg ttcggacttg acggcctctc
      541 ggcgcgggcg ttgcagaagt attactacca tcgctatggg acgacgctgc gcggcctcat
      601 cgaagagcac gcggtcggtc ccgacgaatt cctcgccttc gtgcacgaaa tcgatcgctc
      661 gtcgctcgcg cccaatccgc cgctcgccaa ggaaatcgcc gctctgcccg gccgcaagct
      721 gatcctgacc aacggctcgc gcgaccatgc gctgcgcacc gccgcgcaac tcggcctcag
      781 cgccctgttc gaagatgcgt tcgatatcgt cgccgccaat atggtgccga agccggcgcc
      841 ggaagcgtac gacgcgtttt tcaagaaata cggcgtcgag ccggctaggg ccgccatgtt
      901 cgaggatatc gcccgcaacc tgatcgtgcc gcacgagcgc ggcatgacca cgattctcgt
      961 ccgtccgcgc gccgatcaga tcgactaccg cgaggcgcag gatcgccgcg acgaggattc
     1021 gccggcggtc gacttcgcca ccagcgagct cgccggcttc ctgcaaaggg tgaatctgga
     1081 gctgaggctg gccgatgaat ctcctcgccc cgcgtagcgg agagaaggct atcatccgct
     1141 ttcgctgcgg cgattggcca gcgccacggc cagcgtaatc gccaggcaga acgtcgccga
     1201 cagcgagcgg aaggcgccaa aatcgctttc cgccacttca aatcgcgctt tggcgaggct
     1261 ggcgagcggc gatagggggc tgtcggtgat gaccacgacc ggcacgccgc gcgcggcggc
     1321 gtggcgcgtc agttcgacgg tgaagggcgt atagggggcg aagctgatcg cgatcagcgc
     1381 gtcgtcgcgt ccggcatgag cgagctgctc ggcgccgagg ctgccgacat tgtcgatcag
     1441 ggccgcgccg acgccgagct tggcgagggc gtaagcaagg taatgcgcga ccgggaaggc
     1501 tcggcgctgg ccgagcagat agatggtgcg ggcgtcggcc aaggtttcga tggcgcggtc
     1561 gaaagcctca cgcgttacgc tttcgcgcag gcgcgccagc gaggcggtgg ccgaatcggc
     1621 gaagccggcg agcagatggg tcggatcgcc gctatccatc gccttgtcat gcaaggccat
     1681 catccgcgca ggatagtccg gccagcggtt gcgcaaacgc gagcggaaaa tctcctgcag
     1741 gtcggagaag ccggaaaagc cgatcgactg ggcgaagcga accaaggtcg aagcctggac
     1801 gcgggcatgg ccggcgacgc tggcggcggt gccgagcgcc atctcgtcgg gatgctccaa
     1861 ggcgaaggcc gcgacctgct gcaggcgctt gggcaattcg ggctggattt cgatcagccg
     1921 caatttgagc tcgtcgaaat cgactggcgg agtcatcgaa aaccggctcc tcttgctttc
     1981 gcgaagccgc tcgcgaaaag cgcgtcgaag gcttgacact ggcggcgcca gacggaatga
     2041 aagttctaaa aacaccttct aggagaatgt ttattataga cagcgtgcgt gcgcgagtcc
     2101 tgcgccgccg cggtggcgag agccaaaaag gagacgcgca tgagggcaat cggcgtcggc
     2161 ctgatcggga ccggctatat ggggaagtgc cacgctttgg cctggacggg cgtcaaagcg
     2221 gtgtttggcg acacgcccga catccggctc gcttcgcttt gcgaggtcac gcccgagctc
     2281 gcggccaaga aggcggcgga gttcggcttc gccaagccga ccgccgattg gcgcgccttg
     2341 atcgccgatc ccgagaccga cgtcatctcg gtgacgacgc ccaatcaatt ccacgccgat
     2401 atggcgatcg cggccttgga ggcgggcaag catgtctggt gcgaaaagcc gatggccccg
     2461 aactatgccg acgccgagcg catggcggcg gcggcgcggc gctccggcag ggcggcgatc
     2521 ctcggctaca attacattca gaacccggcg atccgctaca tccgcaaact gctcgacgag
     2581 ggcaggatcg gcgcggtcaa tcacctgcgc atcgagatgg acgaggattt tctcgccgat
     2641 cccgaccagc cgcattcctg gcgcagcgaa gcctccgccg gctacggcgc gctcgacgat
     2701 ttcgccgtcc atcctttgag cctgatttcg acattgttcg gccgcgccga ggcggtgttt
     2761 ggcgaaatgt ccaagcccta tccggaccgc ccgcatcagg gcgggcggcg cgcggtcgaa
     2821 acctacgaca tcgccacggc gctgttgcgc ctgccgggcg ggatcagcgg cgccatccat
     2881 gtcaaccgct gcgcctgggg ccgcaagggc cgcatcttcg ttcagatttt cggcggccgc
     2941 ggcatgatcg ccttcgacca ggagcgcttt aacgaggtgc aggtctacgt cgccgagggg
     3001 cggccggagg atcagggctt ccgcaccatt ctcagcgggc cggcgcacaa gccctacgac
     3061 cgcttcatcg tcgcgcccgg ccatcagctc ggcttcaacg acctgaagat catcgaatgc
     3121 cgcgccttga tcgggcgcat gctcggcgag aagacggtcg cgatcgattt cgacgagggc
     3181 ctcgcgatcg agcgtgcggt gcatgcgatc gcccgcgcca gccgcgaggg ccgatgggta
     3241 caggtgtgag agaggcggcg gctttcctcc tccgcgaagc gggggagggg gaccatgcgg
     3301 agcatggtgg agagggcctc gtcgcctcag gccaatcccc aacgccgcgg cccccgccac
     3361 cgcgctccgc gcggtctccc tcccacgctt cgcgggggag gatcgcggcg atcgacgcgg
     3421 cgcgcggcgc ggcgctgatc ggcatggcgg cgtaccattt cagttgggat ctcgcctatt
     3481 tcggcctcgc cgcgccgaat tttccgctga cgccgccgat gcggctcttc tcgcattgcg
     3541 tcgccggcgc ctttctcgcg ctggtcggcg cgtccctggt tctggcgcat cacgacggct
     3601 tccatcgccg cgcctttcag cgccggctgg cgctgatttg cggcgcggcg gcgctggtga
     3661 cgatcgccac cgccatcctt gcgccgcaag acgcgatcta tttcggcatt ctccattgca
     3721 tcgccgtcgc cagcttgctt gccgcgccgc ttctacagac gcggctctgg gcgcctttcg
     3781 ccgccgcagg cttcgctttt ctcgcgccgg ttctcttcgc cggtccggcc ttcaatcagc
     3841 ccgccgtcat ctggctcggc ctgaatacgg ctccgccgag cacgttcgat tggcggccgc
     3901 tcgcgccatg gtcgggcctg gtctttctcg gcctcgcttt cgcgcgcctc tggtggcggc
     3961 cattgacgga atcgcggctc gcgacatggc gccctcgcgc cgtcgctgcg cgggcgctgg
     4021 cctgggcggg gcggcgcagc ctgccgatct atctcatcca ccagcccgtc ctgttcgcgc
     4081 ttctgttcgc ggcgacgggg ttcgcctcgc ctgacgcgcg gcgcgacgag gtcaatttcc
     4141 gaaacgtgtg cctggcccaa tgcggacgcg ccggcgggac cgcggatcat tgccagcgcg
     4201 gctgccaatg cgtggtcggc ggcttgcgcg acgcaggcct ggcgatcgcc ttctcgcgcg
     4261 cgcatctgga cgacgcgcaa cataagaggt tttcaagcat cgttcaggcc tgcgcccggg
     4321 cgccgtgagt tcttttgtac tttatagctt gaccccccag ccagggcctt catcagccgc
     4381 cacgcggtcg acggcggcgg ctgcaattcc cttctcccgc ttgcgggaga aggtggccgc
     4441 gaagcggccg gaagagggta agccgcgacc gacgcgtctg cttacttcag catccaggcg
     4501 tgatccggat cgttgtagaa ccgccaggcc cgtttcggcc cggccatcac gttcagatag
     4561 tacaattcat agccatgcgg cgcgccgacc gggtgatagc cgcgcggcac cagcaccacg
     4621 tcgcggtcgc tgaccgtcag ggtctcgtcg agagagcggt cgtcggtata gacgcgctgg
     4681 aaagcgaaac cctgcttcgg cgcgatgcgg tgataatagg tctcttcgag ataggattcc
     4741 tccggcagcg cgtcgcgatc gtgcttatgc ggcggatagc tcgaccaatt gccgccgggc
     4801 gtgatcacct cgacgacgag caggctttcg gcggggaccg tctccgacaa gatgttgcgc
     4861 acgaagcggg tgttggagcc ggcgccgcgc gtcgtcgtct cgacctccgc cgggccgatc
     4921 acgcgggccg gcagcttgcc cgtggcgggc gccgtgcaca gggcgatttc gcaatccgtc
     4981 gccgcctcga ccgcataagt cgcgccggcc ggcaggtaga cggaccatgg caggccgtcg
     5041 aagacattgg cgcgctcgcc gaccacgccg gaatcgaaat tcttcgccgt cacccgcgcc
     5101 ttgcccgaca ggaggacgat gcacgcttcg gtcttgccgg tctcgccggc atgacgggcg
     5161 ccttgcttca agtcgcgcgc ctcgaagccg acataggtcc
//
LOCUS       DATGJW010000143         3309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_855905, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000143 DATGJW010000000
VERSION     DATGJW010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3309
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_855905"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3255
                     /locus_tag="VKU03_04375"
     CDS             <1..3255
                     /locus_tag="VKU03_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011969735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase B/oxoprolinase family protein"
                     /protein_id="HLJ70529.1"
                     /translation="KPEQLYADVLEIDERVLADGAIEKTPDLDAVRRDLEAAKAKGFD
                     ALAIVFMHAYRYPEHERQVARLARAMGFAQVSVSHECSALIKLVGRGDTTVVDAYLSP
                     ILARYVEQVAKALDAKRVGARVMFMMSSGGLTAAELFAGKDAILSGPAGGVVAMAETG
                     RSAGFDRVIGFDMGGTSTDVAHFDGEYERDFETEVAGVRMRAPMMKIHTVAAGGGSIL
                     HFDGARLRVGPDSAGANPGPKCYRRGGPLALTDANLTAGKLIPDFFPKIFGPGQDQPL
                     DAASVRAAFERLALEIGDGRTPEETADGFIAIAVAKMAEAIKKISVARGYDVTRYALN
                     CFGGAGGQHACDVADALSIKTILIHPLSSLLSAYGMGLADIRAHRSQGVEAPLDAKAP
                     ALIARLGKALGAEAVKEVAGQGVAKSAIRLNISAQLRYAGADTALGVKAGSLIGMRRA
                     FEAAHRARFGFIDRDKALSVEAVTVEAIGGGARFSERAKAASKARLPAPERAVRFFSH
                     GAWREAKVYLRDRLALGHKVSGPAMIIEPHQTIVVEEGWRAEITGKNHVVLRRVAAKA
                     ERRAIGTKADPVMLEIFNNLFMSIAEQMGVTLQNTAYSVNIKERLDFSCAVFDRDGRL
                     VANAPHMPVHLGSMDRSVETIIRLNEGRIRQGDVFALNAPYNGGTHLPDITVCTPVFD
                     EKGKRILFWVASRGHHADVGGISPGSMSPRATNIHEEGVYIDNFLLIERGRFREKALY
                     ALLESGPYPARNPLQNVNDLKAQIAANEKGAQELRRMVEQFSLRTVEAYMGHVQDNAA
                     ESVRRVIDRLKDSEFSTEMDQGAIIKVKISVDKLKREATVDFTGTSPQQPTNFNAPEP
                     VARAAVLYVFRVMVDDEIPMNVGCLRPIRIVIPEGCMLSPRYPAAVVAGNVETSQAVT
                     DTLFGALHALGSAQGTMNNLTFGNARYQYYETICSGAPAGPGFDGASAVHTHMTNSRL
                     TDPEVLESRFPVVLEDFHIRRFSGGKGRWKAGDGTRRILRFREPMDLAILSGHRRVAN
                     FGVDGGEEGELGRNFVRRLDGRLETLQGCDQTKLEASEAVIIETPTGGGWGRAE"
BASE COUNT          582 a         1111 c         1077 g          539 t
ORIGIN      
        1 aagccggagc agctctatgc cgacgtgttg gagatagacg agcgcgtcct cgccgacggc
       61 gcgatcgaaa agacgcccga tctcgacgcc gtgcgccgcg atctcgaagc cgccaaggcg
      121 aaaggcttcg acgcgctcgc catcgtcttc atgcacgcct atcgctatcc cgagcatgag
      181 cgccaggtcg cgcgcctcgc ccgcgcgatg ggtttcgcgc aagtctcggt cagccatgaa
      241 tgctcggctt tgatcaagct ggtcggccga ggcgatacga ccgtcgtcga tgcgtacctt
      301 tcgccgatcc tcgcccgcta tgtcgagcag gtcgccaagg cgctcgacgc caagcgcgtc
      361 ggcgcgcggg tgatgttcat gatgtcgtcc ggcggcctca ccgcggccga gctcttcgcc
      421 ggcaaggacg cgatcctgtc cggcccggcc ggcggcgtcg tcgcgatggc cgaaaccggc
      481 cgcagcgccg gcttcgaccg cgtcatcggc ttcgacatgg gcggcacatc gaccgacgtc
      541 gcccatttcg acggcgaata cgagcgcgat ttcgagacgg aagtcgccgg cgtgcgcatg
      601 cgcgcgccga tgatgaaaat ccacaccgtc gccgccggcg gcggctcgat ccttcatttc
      661 gacggcgcgc gcttacgcgt cggaccggat tcggccggcg ccaatcccgg cccgaaatgc
      721 taccgccgcg gcggcccgct cgctttgacc gacgccaatc tgacggccgg caagctgatt
      781 ccggatttct ttccgaaaat cttcggcccc ggacaggacc agccgctcga cgcggcgagc
      841 gtgcgcgcgg ctttcgagcg gctggctctt gagatcggcg acggccgcac gcccgaagag
      901 accgccgacg gctttatcgc catcgccgtc gccaaaatgg ccgaggcgat taagaaaatc
      961 tcggtcgcgc gcggctatga cgtaacccgc tatgcgctca attgcttcgg cggcgccggc
     1021 ggccagcatg cttgcgacgt cgccgacgcg ctgtcgatca agacgatttt gatccatccg
     1081 ctgtcgtcgc ttctctcggc ctatgggatg gggcttgccg acatccgcgc ccatcgcagc
     1141 cagggcgtcg aagcgccgct cgacgccaaa gcgccggcgc tgatcgccag gctgggcaag
     1201 gcgctcggcg ccgaggcggt caaggaagtg gcgggacaag gcgtcgccaa atcggcaatc
     1261 aggctcaata tcagcgcgca attgcgctat gccggcgccg acaccgcgct cggcgtcaag
     1321 gcgggttcac tcatcggcat gcgccgcgct ttcgaggccg cacatcgcgc gcgcttcggt
     1381 ttcatcgacc gtgacaaggc gctcagcgtg gaagcggtga cggtcgaagc gatcggcggc
     1441 ggagcacgct tttccgagcg cgcgaaagcg gcgagcaaag cgcgcctgcc cgcgccggag
     1501 cgcgcggttc gcttcttttc gcacggcgcg tggcgggagg cgaaagtcta tcttcgcgac
     1561 aggctcgctc ttggccacaa ggtgagcggc ccggcgatga tcatcgagcc gcatcagacg
     1621 atcgtcgtcg aagaaggttg gcgcgccgag attaccggga agaatcacgt cgtgctgcgc
     1681 cgcgtcgccg ccaaggcgga gcgccgggcg atcgggacca aggccgatcc ggtcatgctc
     1741 gaaatcttca acaacctgtt catgtcgatc gccgagcaga tgggcgtgac gctgcagaac
     1801 accgcctatt ccgtcaacat caaggagcgg ctcgatttct cctgcgcggt gttcgaccgc
     1861 gacggccggc tcgtcgccaa cgcgccgcat atgccggtcc atctcggctc gatggaccgc
     1921 tcggtcgaga cgatcatccg tctcaacgag ggccgcatcc gccagggcga cgtcttcgcg
     1981 ctgaacgcgc cttacaatgg cggcacgcat ctgcccgaca tcaccgtctg cacgccggtc
     2041 ttcgacgaga agggcaagcg gatcctgttc tgggtcgcct cgcgcggcca tcacgccgat
     2101 gtcggcggaa tctcgcccgg ctcgatgagc ccgcgcgcga ccaacatcca cgaagagggc
     2161 gtctatatcg acaatttcct gctcatcgag cgcggccgct tccgcgagaa ggcgctttac
     2221 gccctgctcg aaagcggtcc gtacccggcg cgcaatccct tgcagaacgt caacgacctc
     2281 aaggcgcaga tcgccgccaa cgagaagggc gcgcaggaat tgcggcgcat ggtcgagcaa
     2341 ttctcgcttc gcaccgtcga agcctatatg ggccacgtcc aggacaacgc cgcggaaagc
     2401 gtacgccgcg tgatcgaccg cctgaaggac agcgaatttt ccacggaaat ggaccagggc
     2461 gcgatcatca aggtcaagat cagcgtcgac aagctgaagc gcgaagcgac tgtcgacttc
     2521 accggcacat cgccgcagca gccgacaaat ttcaacgcgc cggagccggt ggcgcgcgcc
     2581 gccgtgctct acgtcttccg cgtcatggtc gacgacgaga ttccgatgaa cgtcggctgc
     2641 ctgcggccga tccgcatcgt cattccggaa ggctgcatgc tgtcgccgcg ctatcctgca
     2701 gctgtcgtcg ccggcaatgt cgagacttcg caagccgtga ccgacacgct gttcggcgcc
     2761 ttgcatgcgc tcggctcggc gcagggcacg atgaacaatc tcaccttcgg caatgcgcgc
     2821 tatcagtact acgagacgat ctgctcgggc gcgccggccg ggccgggttt cgacggcgcc
     2881 agcgcggtcc atacccatat gaccaattcg cgcctgaccg atccggaagt gctggaatcg
     2941 cgctttcccg tcgtgctgga ggatttccat atccgccgct tctcgggcgg caagggacgc
     3001 tggaaggccg gcgacggcac gcgccggatc ttgcgcttcc gcgagccgat ggacctcgcc
     3061 atcctgtccg gccatcgccg tgtcgccaat ttcggcgtcg acggcggcga ggagggcgag
     3121 ctcggccgca atttcgtgcg gcgcctcgac gggcggctgg agacgctgca aggctgcgat
     3181 cagaccaagc tcgaagcaag cgaagcggtg atcatcgaga cgccgaccgg cggcggctgg
     3241 ggacgggcgg agtgagggag gaactccctt ctccccgcaa agcggggaga aggtggcgcg
     3301 aagcgccgg
//
LOCUS       DATGJW010000144         8156 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_25965, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000144 DATGJW010000000
VERSION     DATGJW010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8156)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8156)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8156
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25965"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VKU03_04380"
     CDS             <1..861
                     /locus_tag="VKU03_04380"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ70530.1"
                     /translation="DSFFPPLYQGAQDAAKALGVKLNYIPIDEADIEATSARTMQTAI
                     AQKPDAIVVGDFVTSVVDPYIKQAVAAGIPVYVDQSGQDQWQADGAFGFVGQQGPEVG
                     KVAAERLIKAGSKNILCVINVPGNPYLQAICKGLAEKSKELGATSSNLELPTADSTDQ
                     GKVSRDIGAYLQSHRSVDAIFTENAAVGTAATAAVEAAQMTGKVHVGTMEISRVALQQ
                     IQNGKLDFLVNEQPYLDGYFGVVFATLYVKYGLAPVGPVATGPSIVDKKNIDKILKVF
                     DTYPNVIGSK"
     gene            916..1983
                     /locus_tag="VKU03_04385"
     CDS             916..1983
                     /locus_tag="VKU03_04385"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70531.1"
                     /translation="MLASPDQTNIAEARPAESFDDAASTEPVRRRSRLTGFLLRPEAG
                     GVVSALLVFIFFAIAAGQNGFLTVLGTANWLDTASDLGIIALPVGMLMIGGEFDLSVG
                     SVVGASSMIVAICSGYYSLNPWIGVAIAFAFAAAVGVANGLIVVRTRLPSFIVTLATM
                     LMVMGGMLGASIALTGSSSLSAVPSGSAQAVFASNLLQFHISIVHWLVLTAIAAYVMQ
                     RTAFGNWVYATGGNSVTARLAGVPVDRVKIILFVCTALAAALSAANETLTFQNGNITL
                     GSQYVFTGIAAAVIGGVLLTGGYGSPVGTVFGSLTYGIVSLGVFFLGWNADLANFFVG
                     LLLLLAVLANHRLRQLAMGRA"
     gene            1986..2777
                     /locus_tag="VKU03_04390"
     CDS             1986..2777
                     /locus_tag="VKU03_04390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008120493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ70532.1"
                     /translation="MAAPIVELRGVTKRFVGVTAIADLSLQLFPGEVFCLLGENGAGK
                     STVIKILTGVEAPTKGDVLVDGETVHFASPREAREKGIATVYQEIGTLPLMSVARNFV
                     LAAEPQKGRGLFRRLDLDEAARIALKSLRDLGISRVDDGDQLVGTLSGGERQALAIGR
                     AIHFGARVLVLDEPTAALGVRESATVLKLIGRVRSRGVAVLFITHNAYHAYASGDRFM
                     VLRRGRALAAFNKNERTIGEVIELMAGGAELKSLLTSSEAAIGLS"
     gene            3022..4281
                     /locus_tag="VKU03_04395"
     CDS             3022..4281
                     /locus_tag="VKU03_04395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLJ70533.1"
                     /translation="MSIHQSGRVLLSACIVAAVGAWGTTAIAQEDWIGTWAASAQPVW
                     GPDFLAPPKVPRNLWNQTVRELAHVSIGGSKVRLLLTNEYTSHPITIGAVHVAISNKG
                     ASILLGSDRAVTFGGKTSVAIPPGAPALSDPVDLTVAPLSDLAVSFYLPNVTPIETMH
                     WEGRQTAYIAAGDKTAEPDIKPDATMTAKVFLSEILVDAPAEPRAVVTFGDSITDGDG
                     STVDANHRWPDDLAQRLADRRIAVLNEGISGAKVLSDRMGVNALARFDRDVLSQLHAD
                     VVTMMMGINDIGWPDSVLAPNDKVPTAEEVIAGHQQLIARAHLHHMRIIGATLTPFED
                     TFHGTPLFGYYSEEKEKVREAVNKWIRESGAYDAVADFDAVVRDPANPKHLKPEYDSG
                     DHLHPNDAGYVAMANSINLDALLGNKK"
     gene            complement(4469..5455)
                     /locus_tag="VKU03_04400"
     CDS             complement(4469..5455)
                     /locus_tag="VKU03_04400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ70534.1"
                     /translation="MSRLTTLSRMLALGVGAAALSVGVADAAPLVKECGKNGQYVIGF
                     SQANNAEPYRQHVNDELTAAAKKVPQFTLQIADGAGNVNTQTSQMDNFITQKVDLILI
                     SPFEASPLTPVVARAMKAGIPVIELDRKTVGEPGKDYTAFIGGDNFKIAEQAGKYVAE
                     KLLPGGGDVAVLEGLPSSTPAVERLNGFKEGVKENAKIHVVAEQAADWLPDKAQTAFA
                     AMLQAHPEIKAVYASNDMMAAGARLAAKGAGKKDGDIKILGTDGLPGPAGGIRAVAEG
                     EWAATFTYPTGGPEAIEMAKSILLDCAEKVPTVVTVPTTAITPENAKKMMGN"
     gene            complement(5565..6623)
                     /locus_tag="VKU03_04405"
     CDS             complement(5565..6623)
                     /locus_tag="VKU03_04405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003591973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70535.1"
                     /translation="MNDAATLRESAPARPAPPPRRRLDFFAVAVRFQSVIGLLVVAIG
                     GVIFSPRRHGMILFLAPDNMANIVRAVSETGIIAVGMTFVIITAGIDLSVGALLSLSS
                     VLTATIMTTAGWDLAPTLLFVMVCGAAFGAAQGIISTRFRLEPFIVTLAGLQVARGLA
                     LVISGNQYINISYGHGPGLAPPIFSVLGDRLFGNIVPVATLVFVVVAFVATFVLNFTR
                     FGRYVFAVGGNERAARLSGVPVTLVKISVYAITGLVSAIAGIVHAGQFNFGSANDGTG
                     YELTAIAAVVIGGTSLFGGSGSMVGTVAGAVMLGALANVLQLNNINAALQLLATGAII
                     VLAAVLQSLVRRREGLGR"
     gene            complement(6616..8151)
                     /locus_tag="VKU03_04410"
     CDS             complement(6616..8151)
                     /locus_tag="VKU03_04410"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003591976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ70536.1"
                     /translation="MSAAEAPLLAMRGVAKSFGGAQALRGVDFDLQRGEVHALLGENG
                     AGKSTLMNVLSGVIEPDEGEILIDGSSVRFADPRAAQAAGVATIFQELDLVPSLDVTA
                     NLFLGREMTRGGVLDRGAMRRAARTRLEAIGFDLDVDQPVGELSVGRRQIVAIAKALT
                     YASRILVMDEPTAALTAAEVQRLFETMRELAGRGVGVVYISHRLEEVPRVADRVTVLR
                     DGKVAGTASPTAPQSDLVALLVGRPLTELYPRRGGTLGRRVLRLERAAYRPRRQRAGW
                     RAPRDVSLEVRAGEVVGLAGIMGAGRTELLSALYGAGGPGAWSGIVEIEHKPAALNSI
                     RAARRAGLAFVSDDRRGGGLMLRDSVGRNLVASVLRRVSPFFLMSKLREARLARQAIA
                     SFDVRPPRPELAVGALSGGNQQKVVLAKEVLGAPRLLLLDEPTRGVDVGAKGEIYARI
                     RHLAAEGVGVLVASSEMPELIGLCDRIVVLRGGRTVAEFPAGAAEREILDAAESLKDD
                     SDE"
BASE COUNT         1398 a         2725 c         2654 g         1379 t
ORIGIN      
        1 gattccttct tcccgccgct ctatcagggc gcgcaggacg ccgccaaggc gctcggcgtc
       61 aagctcaact acatcccgat cgacgaggcc gatatcgagg cgacctcggc ccggaccatg
      121 cagacggcga tcgcacagaa gccggacgcc atcgtcgtcg gcgacttcgt caccagcgtc
      181 gtcgatcctt atatcaagca agcggtggcg gccggcattc cggtctatgt cgatcaatcc
      241 ggccaggacc aatggcaggc cgacggcgcc ttcggctttg tcggccagca agggccggaa
      301 gtcggcaaag tcgcggccga acgcctgatc aaggccggat cgaagaacat tctttgcgtc
      361 atcaacgtgc cgggcaatcc gtacctccag gcgatctgca aaggcctcgc cgagaaatcc
      421 aaggagctcg gcgcaacgtc gagcaatctg gagctgccga ccgcggattc gaccgatcag
      481 ggcaaggtca gccgcgacat cggcgcctat ctccagtcgc atcgcagcgt cgacgcgatc
      541 ttcaccgaga acgccgcggt cggaaccgcc gccaccgccg cggtcgaggc ggcgcaaatg
      601 accggcaagg tccatgtcgg cacgatggag atttcgcgcg tcgccctgca gcagatccag
      661 aatggcaagc tcgatttcct ggtcaacgag cagccctatc tcgacggcta tttcggcgtc
      721 gtcttcgcca cgctctacgt caaatacggc ctcgccccgg ttggaccggt cgcgaccggg
      781 ccgagcatcg tcgacaagaa gaacatcgac aagattctca aagtgttcga tacctatccg
      841 aatgtcatcg gctcgaaatg agctcgagcg gcgagtcttg acaggaccac cgcatttctt
      901 cggccgagcg cggcaatgct cgcaagtcca gaccagacga atatcgccga ggcgcgtccg
      961 gccgagtcct tcgacgacgc cgcctcgacc gagcccgtgc ggcggcgcag ccgcctgacc
     1021 ggcttcctgc tgcggcccga ggcgggcggc gtcgtctcgg cgctgctcgt cttcatcttc
     1081 ttcgcgatcg ccgccggaca gaacgggttc ctcaccgtgc tcggcacggc caattggctc
     1141 gacaccgcct ccgacctcgg catcatcgcg ctgccggtcg gcatgctgat gatcggcggc
     1201 gagttcgatc tttcggtcgg ctcggtcgtc ggcgcgtcct cgatgatcgt cgcgatctgc
     1261 tcgggctatt attcgctcaa tccgtggatc ggcgttgcga tcgccttcgc tttcgcggct
     1321 gccgtcggcg tcgccaacgg actgatcgtt gtcaggacgc gattgccctc cttcatcgtc
     1381 accttggcga cgatgctgat ggtgatgggc ggcatgctcg gcgcgtcgat cgctttgacc
     1441 ggctcctcca gtctgtcggc cgtaccgtca ggctcggcgc aggcggtttt cgcctccaat
     1501 ctcctgcaat tccacatttc gatcgtccat tggctcgtcc tcaccgccat cgccgcctat
     1561 gtcatgcagc gcaccgcctt cggcaattgg gtctacgcca ccggcggcaa ttcggtgacg
     1621 gcgcgcctcg ccggcgtgcc ggtggatcgg gtgaagatca tcctcttcgt ctgcaccgcc
     1681 ctcgccgcgg cgctctcggc ggccaacgag acgctgacct tccagaacgg caacatcacg
     1741 ctcggctccc agtacgtctt caccggcatc gccgccgcgg tgatcggcgg cgtcttgctg
     1801 accggcggct atggctcgcc ggtcggcacg gtgttcggat cattgaccta cggcatcgtc
     1861 tcgctgggcg tgttctttct cggctggaac gccgacctcg ccaatttctt cgtcggcctc
     1921 ctcttgctgc tcgcggtgct cgccaatcat cgcctgcggc aattggcgat ggggagggct
     1981 taaggatggc cgcgccgatc gtcgaattgc gcggggtgac gaaacgcttc gtcggcgtga
     2041 ccgcgatcgc cgatctttcg ctgcagcttt ttccgggcga ggtcttttgc ctgctcggcg
     2101 aaaacggcgc cggcaagtcg acggtgatca aaatcctcac cggggtcgag gcgccgacga
     2161 aaggcgacgt actggtcgac ggcgagacgg tccatttcgc cagccccaga gaggcgcggg
     2221 aaaagggcat cgccaccgtc taccaggaga tcggcacgct gccgctgatg agcgtcgcgc
     2281 gcaatttcgt cctcgccgcc gagccgcaga agggacgcgg cctgttccgc cgtctcgacc
     2341 tcgacgaagc cgcgcgcatc gcgctcaaga gcttgcgcga cctcggcatc agccgcgtcg
     2401 acgacggcga tcagctcgtc ggcacgctgt cgggcggcga acggcaggcg ctcgccatcg
     2461 gccgcgccat tcatttcggc gcccgcgtgc tggtgctgga cgaaccgacc gccgcgctcg
     2521 gcgtccggga atcggcgacc gtgctgaagc tgatcgggcg cgtgcgctcg cgcggcgtcg
     2581 ccgtcctgtt catcacccac aacgcctatc acgcctatgc ttccggcgac cgcttcatgg
     2641 tgctgcggcg cggccgggcg ctggcggcgt tcaacaagaa cgagcggacg atcggcgagg
     2701 tcattgaatt gatggccggc ggcgcggagt tgaagtcgct gctgacgagc agcgaggcgg
     2761 cgatcgggtt gtcgtaaagg accaacgatc ccgtaggggg ttgaagcgct tgggtcgtca
     2821 tttgcgggga tcccgagcgc gccgacgggt acgaagcaat ccactcgcag agctcagtcc
     2881 tgtgaatgga tcgcttcgct cgccatggcg gggttttcga gagttcggcg ccgaaatcgt
     2941 ctcgcgccga aagaaaggaa aggagctcat cgagagttca tttgctgcgg ttctgcccaa
     3001 tcgatcaaag cggaggcaaa gatgagtatc catcagtctg gacgcgtcct tttgtctgct
     3061 tgcatcgtcg ccgccgtcgg cgcgtgggga acgacggcca tcgcgcagga ggattggatc
     3121 ggcacatggg cggcgagcgc tcagccggtt tgggggccgg atttcctggc gccgcccaaa
     3181 gtcccgcgca atctctggaa ccagaccgtg cgggaattgg cccatgtctc gatcggcgga
     3241 tcgaaggtcc gcctgcttct gactaacgaa tatacgagcc acccgatcac gatcggcgcg
     3301 gttcacgtcg ccatctcgaa caagggcgct tcgatcctgc tcggctcgga tcgcgcggtc
     3361 acgttcggcg gcaagacctc ggtcgccatc ccgcccggcg cccccgcctt gagcgatccg
     3421 gtcgacctga ccgtcgcgcc gttgtcggat ctcgccgtca gcttctatct gccgaatgtg
     3481 acgccgatcg agacgatgca ttgggagggt cggcagacgg cctatatcgc ggccggagac
     3541 aagaccgccg agccggacat caagcccgac gcgacgatga cggcgaaggt cttcttgagc
     3601 gaaatcctcg tcgacgcgcc ggccgagccg cgcgccgtgg tgacgttcgg cgattcgatc
     3661 acggacggcg acggctcgac cgtcgacgcc aaccatcgct ggccggacga tctcgcccag
     3721 cggcttgccg atcgcaggat cgcggtgctg aacgaaggca tttccggcgc aaaggtcctg
     3781 tccgaccgga tgggcgtgaa cgcgctcgcc cgcttcgacc gcgacgttct gagccagctg
     3841 cacgccgacg ttgtcacgat gatgatgggc atcaacgata tcggctggcc ggacagcgtg
     3901 ctggcgccca acgacaaggt tccgaccgcg gaagaggtca tcgccggcca tcagcagctc
     3961 atcgcccgcg cccacctgca tcatatgcgc atcatcgggg cgacgctgac gccgttcgag
     4021 gacacgttcc acggcacgcc gctcttcggc tattacagcg aggagaagga gaaggttcgg
     4081 gaggcggtga acaaatggat ccgcgagagc ggcgcctatg acgcggtagc cgatttcgac
     4141 gcggtcgtgc gcgacccggc caacccgaag catctcaagc cggaatacga ttccggcgat
     4201 catctgcatc cgaacgatgc cggctatgtg gcgatggcca attcgatcaa tctcgacgcg
     4261 ttgctcggga acaagaaata gccgtcgacg cggcggcgct tcgtcgccgt ccgggcgagg
     4321 agcgaagcaa cggcgcaatc tccttgaggc cgcgcctaac gaatggattg cttcgcttcg
     4381 ccggcaatga cggttttggc gcgcgcggcg gcgcggttct gatccctgaa ccgcgccgcc
     4441 gcctttgagc gaaacgggag gttttcgctc agttccccat catcttcttg gcgttttccg
     4501 gcgtgatcgc cgtggtcggc accgtcacca cggtcggcac tttctcggcg caatcgagca
     4561 ggatcgactt cgccatctcg atcgcctcgg gcccgccggt cgggtaggtg aacgtcgccg
     4621 cccactcgcc ttccgcgacg gcgcgaatgc cgccggccgg cccaggcagg ccgtccgtgc
     4681 cgaggatttt gatgtcgccg tccttcttgc cggcgccctt ggcggcaagc cgcgcgccgg
     4741 cggccatcat gtcgttcgag gcgtagaccg ccttgatctc cggatgggct tgcagcatcg
     4801 cggcgaaagc ggtctgcgct ttatccggca gccagtcggc ggcctgttcg gcgacgacgt
     4861 gaatcttggc gttttccttc acgccctcct tgaagccgtt gagccgctcg accgccggcg
     4921 tcgagctcgg caggccttcc agcaccgcga cgtcgccgcc gcccggcagc agcttctcgg
     4981 cgacgtactt tcccgcctgc tcggcgatct tgaaattgtc gccgccgata aaggcggtgt
     5041 aatccttgcc cggctcgccg accgtcttgc ggtcgagctc gatcaccgga atgccggcct
     5101 tcatcgcccg cgccacgacc ggggtcagcg gcgaagcctc gaagggcgag atcagaatca
     5161 ggtcgacctt ctgcgtgatg aaattgtcca tctgcgaggt ttgcgtgttc acgttgccgg
     5221 cgccgtcggc gatctgcaag gtgaattgcg gaaccttctt cgccgcggcg gtcaactcgt
     5281 cattgacgtg ctggcgatag ggctcggcat tgttggcttg tgaaaagccg atcacatatt
     5341 ggccgttctt gccgcattcc ttgacgagcg gcgcggcgtc agccacgccg acgctaagcg
     5401 cggcggcgcc gacgccgagc gccagcattc tggatagtgt tgtgagacga ctcatcagtt
     5461 cactccccga gattcgtgtg tttcaaagat gaatttctcc cgaatggaca tcgggtagcg
     5521 tcgcgcccgg cgcagcgccg agccacgact ttttggccgc ctcgttaccg acccaatccc
     5581 tcgcgacgac gtacgagcga ctgaagcacc gcggccagca caatgatcgc gccggtcgcg
     5641 agcaattgca gggccgcatt gatattgttg agctgcaaaa cattggcgag cgcgccgagc
     5701 atgaccgcgc cggcgaccgt gccgaccatc gagcccgagc cgccgaacag gctggtgccg
     5761 ccgatcacca ccgcggcgat cgcggtcagc tcgtaccccg tcccgtcatt ggcgctgccg
     5821 aaattgaact ggccggcatg gacaatgccg gcgatcgccg agacgaggcc ggtaatcgcg
     5881 tagacggaga ttttgaccag cgtcaccggc acgccggaaa gtcgcgccgc gcgttcattg
     5941 ccgccgaccg cgaaaacata gcggccgaac cgggtgaagt tcagcacgaa ggtggcgacg
     6001 aacgccacga cgacgaagac gagcgtcgcc accggcacga tattgccgaa gaggcggtcg
     6061 ccgagcactg agaagatcgg cggcgccagg cccggtccgt gaccatagga aatgttgata
     6121 tattggttgc cggaaatgac cagggccagg ccgcgcgcca cctgcaagcc ggccaaggtg
     6181 acgatgaacg gctcaagacg gaaccgcgtc gagatgatcc cctgcgcggc gccgaaagcc
     6241 gcgccgcaga ccatgacgaa aagcagcgtc ggcgcgagat cccagccggc ggtggtcatg
     6301 atcgtcgcgg tgagcacgct ggacagcgag agcagcgccc cgaccgagag atcgatgccg
     6361 gcggtgatga tcacaaaagt catgccgacg gcgatgatcc ccgtctccga gacggcgcgg
     6421 acgatgttcg ccatattgtc cggcgcaagg aacaggatca tgccgtgccg gcgcggggag
     6481 aagatcacgc cgccgatcgc gacgacgagc aggccaatca cgctttggaa gcggaccgcg
     6541 acggcgaaaa aatcgaggcg ccggcgcggc ggcggcgctg gtcgcgccgg cgcggattcg
     6601 cggagcgtgg cggcgtcatt catcgctgtc gtccttgagg gattcggctg cgtccaaaat
     6661 ctcgcgctcc gcggcgccgg ccggaaattc ggcgaccgtc cgtccgcctc tcaagacgac
     6721 gatgcggtcg caaagaccga tcagctcggg catttcgctg gacgcgacga gcacgccaac
     6781 gccctcggcg gcgagatggc gaatgcgggc gtagatctcg cccttggcgc cgacatcgac
     6841 gccgcgcgtc ggctcgtcga gcaaaagcag acgcggcgcg ccgagcactt ccttggccag
     6901 cacgaccttt tgctggttgc cgccggacag cgcgccgacc gcgagttcgg gccgcggcgg
     6961 gcgcacgtcg aacgaggcga tcgcctggcg cgcaagcctc gcctcgcgca gcttcgacat
     7021 gaggaagaac ggcgagacgc gccggagcac cgaggcgacg agattgcgtc cgacgctgtc
     7081 gcgcagcatc aaaccgccgc cgcgccggtc gtcgctgacg aaagcgaggc cggcgcggcg
     7141 cgcggcgcgg attgaattca gcgccgccgg cttgtgctcg atttcgacga ttccagacca
     7201 ggcgcccggt ccgcccgcgc cgtaaagcgc cgagagaagc tcggtgcggc cagcccccat
     7261 gattccggcc aggccgacca cctcgccggc gcgcacttcg agcgacacgt cgcgcggcgc
     7321 gcgccagccg gcgcgctgcc ggcgcggccg gtacgccgcc cgctccaagc gcagcacgcg
     7381 ccggccaagc gtgccgccgc ggcgcggata gagctcggtc agcggccggc cgaccagcag
     7441 cgcgacgaga tcggattgcg gcgcggtcgg cgaagcggtc ccggcgacct tgccgtcgcg
     7501 caggacggtg acccggtcgg cgacacgcgg cacttcttcg agccggtgcg agatatagac
     7561 gacgccgacg ccgcgcccgg cgagctcgcg catcgtctcg aacaatcttt gcacctcggc
     7621 tgcggtcaaa gccgcggtcg gctcgtccat gacgaggatg cgcgaagcgt aggtcagcgc
     7681 cttggcgatg gcgacgatct gtcggcggcc gaccgacaat tcgccgaccg gctggtcgac
     7741 atcgaggtcg aagccgatcg cctccagccg cgtccgcgcc gcgcggcgca tcgcgccacg
     7801 gtccagcacc ccgccgcggg tcatttctcg gccaaggaac agattggcgg tgacgtcgag
     7861 gctcggcacg agatcgagct cctggaagat cgtcgcgacg cccgccgctt gcgcggcgcg
     7921 cggatcggcg aagcgtacgc ttgaaccgtc gatgagaatc tcgccctcgt ccggctcgat
     7981 cacgccggaa agcacgttca tcaaggtcga cttgccggcg ccgttctcgc cgagcagcgc
     8041 atggacttcg ccgcgctgca gatcgaaatc caccccacgc aaggcctgcg cgccgccaaa
     8101 gctcttggcg acgccgcgca tggcgaggag cggcgcctcg gcggcgctca tttcgg
//
LOCUS       DATGJW010000145         3146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1165203, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000145 DATGJW010000000
VERSION     DATGJW010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3146
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1165203"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            302..886
                     /locus_tag="VKU03_04415"
     CDS             302..886
                     /locus_tag="VKU03_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70537.1"
                     /translation="MSVASRASAAPIGAPINLINFAWVALAFAVMAAAIAIGNLWFLN
                     WIHVLAGGLWTGIDLFMGFVVGPILRAAPFEARRAVITRLTPKTLFIMPTLSIVTGTS
                     GWFLAKQLGFLDVGYPQFWWVAAALAIVTLLTVQGLGYLLPTNLRVYFELRKDNPDGA
                     KIGALMSRYFYAVAFQGAMQVAIIVVMARFATGL"
     gene            complement(917..2521)
                     /locus_tag="VKU03_04420"
     CDS             complement(917..2521)
                     /locus_tag="VKU03_04420"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HLJ70538.1"
                     /translation="MCGIAAAIGWDDAEATVRRVLKGLAHRGDIDDPVVSPLNGIALG
                     TRRLRIVDAERAIQPQASFDGRILVSFNGEIYNHADLRRDLEAKGVAFRTASDTEVIA
                     SGLRIWGPQLLQRLNGMYAFVALDAANGEFLAARDPFGVKPLYLIQSGPRYLFASEMR
                     PLLAATPQGDALLLPPGHLLTRNAVLKFAAAPGGPARPRGAHDPRKLDQLLAAAVHRR
                     LPSDLPCALMFSGGIDSTLVAHYARQIRPDISGYFLGEESAPDYRYAALYAEATGFDL
                     KRVSFGWDGETESRIGKVVAAVETFEPSVVRDALCTSLICERIAEDGYRVALSGEGAD
                     ELFAGYAPLELAFADSDEVGAFVRDQCLLDMHRTNLQRLDRCSMRFGLEAREPFLDPD
                     IVDYALSLGAGDLIGEADGAPQGKAALRSLWSLHPGAIPEAIRDRRKSPLHVGSGFDR
                     SQKISPWIDFAEAAISDGDFAEGRKRFARFDLRSKEELLYLMKLAESFDVFRVPHLTA
                     RPRLRFPAAERQAAVKERLSDFLVEA"
     gene            2732..2983
                     /locus_tag="VKU03_04425"
     CDS             2732..2983
                     /locus_tag="VKU03_04425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70539.1"
                     /translation="MTIHSRSLVGVFDDRDTRIMGAAYRMACDMLDANGGDAAERSLR
                     FEIAAALLEAADDGFSDAPALAQAAVDLVFVDAIEIDFS"
BASE COUNT          515 a         1031 c         1067 g          533 t
ORIGIN      
        1 gcgatcatcc agcccgaggc ggtgtcgaac accgccgtgc cgagattggc gacgacgctc
       61 gccgtccaga tcgccgtgaa agccgcatgg ccgaacgcgc cccagcggcc aggcgcgggc
      121 gaagcgggcg ccgcggggag attggcgtcg acgctcaagg cggctccctg aatgttccgg
      181 cgcgcatcgg gccacgcgga gtcccggctg tcaaatgggc tggcgcagcc cgtcgctctt
      241 cgccgcttcc gcaaattccc gtcttggtct accgtcgacg agcctggcgg gggagcttgc
      301 aatgagcgtg gcgtcgagag cttcagcggc gccgatcggc gcgccgatca atctcatcaa
      361 cttcgcctgg gtcgcgctcg ctttcgcggt gatggcggcg gcgatcgcga tcggcaatct
      421 gtggttcctg aattggattc acgtccttgc cggcggccta tggaccggca tcgacctgtt
      481 catgggcttc gtcgtcgggc cgatcctgcg cgccgccccg ttcgaggcgc gccgcgcggt
      541 gatcacgcga ttgacgccga agacgctgtt catcatgccg accttgtcga tcgtcaccgg
      601 aaccagcggc tggttcctcg ccaagcagct cggctttctc gatgtcggct atccgcaatt
      661 ctggtgggtc gccgcggcgc tcgccatcgt cacgctcctg accgttcagg gcctcggcta
      721 cctcctgccg accaacctgc gcgtctactt cgagctgcgc aaggacaatc ccgacggcgc
      781 caagatcggc gcgttgatga gccgctattt ctatgcggtc gctttccagg gcgcgatgca
      841 agtcgcgatc atcgtggtga tggcgcggtt cgcgacggga ttgtgacggg cgccgcgctc
      901 ttcgcaatga ccgcgcctac gcctcgacca ggaaatcgct gagccgctcc ttgaccgccg
      961 cctgcctctc ggccgccgga aaccgcaggc gcggccgcgc ggtcagatgc ggaacacgga
     1021 acacgtcgaa actctcggcg agcttcatca ggtagagcaa ctcctccttg ctgcgcaggt
     1081 cgaaccgggc gaagcgcttc cggccttcgg cgaaatcgcc gtcggaaatc gccgcttcgg
     1141 caaaatcgat ccagggcgag atcttctggc tgcggtcgaa gcccgagccg acatggagcg
     1201 gcgacttgcg gcggtcgcgg atcgcttccg gaatcgcgcc gggatggagg gaccacaggc
     1261 tgcgcagcgc cgccttgcct tgcggggcgc cgtccgcctc gccgatcagg tcgccggcgc
     1321 cgaggctgag cgcatagtcg acgatgtccg ggtcgagaaa aggctcgcgc gcctccaggc
     1381 cgaagcgcat cgagcagcgg tcgagacgct gcagattggt gcgatgcatg tcgaggaggc
     1441 actgatcgcg gacaaacgcg ccgacttcgt cgctgtcggc gaaggccaat tccagcggcg
     1501 catagccggc gaacaattcg tccgcgcctt cgcccgacag agcgacgcga tagccgtctt
     1561 cggcgatgcg ctcgcagatg agagacgtgc agagcgcgtc gcgcacgacg ctcggctcga
     1621 acgtctcgac cgcagcgacc accttgccga tccgcgattc cgtttcgccg tcccagccga
     1681 acgaaacgcg tttgaggtcg aagcccgtcg cctcggcgta gagcgccgcg tagcgataat
     1741 ccggcgcgct ttcctcgccg aggaaatagc cggaaatatc cggccggatt tgccgcgcgt
     1801 aatgggcgac cagggtcgaa tcgatgccgc cgctgaacat cagcgcgcaa ggaagatcgg
     1861 acggcaggcg gcgatggacc gccgcggcca gcaattggtc gagctttcgc gggtcgtgcg
     1921 cgccgcgcgg ccgggccggg ccgccgggcg cggcggcgaa tttcagcacg gcgttgcgcg
     1981 tcagcagatg gcccggcggc agaagcagcg cgtcgccctg cggcgtcgca gcgaggagcg
     2041 ggcgcatttc cgatgcgaac aaatagcgcg ggccggattg gatcagataa agcggcttga
     2101 cgccgaacgg atcgcgcgcg gcgagaaatt cgccgttcgc cgcgtcaagc gccacgaaag
     2161 cgtacatgcc gttgaggcgt tggagaagct gcggccccca gatgcgcagg cccgaggcga
     2221 tgacctcggt gtcggaagcg gtacggaacg cgaccccctt ggcttcgaga tcgcgccgca
     2281 gatcggcgtg gttgtagatc tcgccgttga aggagacgag gatgcggccg tcgaacgagg
     2341 cctgcggctg aatcgcgcgc tcggcgtcga cgatgcgcaa gcggcgcgtg cccaaggcga
     2401 ttccattcaa cgggctaacg acgggatcgt cgatgtcgcc gcgatgggcg aggcccttca
     2461 gaacgcggcg caccgtcgcc tccgcgtcgt cccaaccgat tgccgccgct atgccgcaca
     2521 tattccgccc cctcgcctta atgcgcatcc tgatgtgccg cttggagctt gggcggggct
     2581 ggagcgcgta ttttttcggc ggcgatcgcg gagcgttccg cagcgcagca tctcgcgaac
     2641 gtgactggtt gcagccaagc gttcatgaga gattttgaga aaccgagacc aatcgcgctc
     2701 ccgcccgact tgcgggaaaa ggagttccgc gatgacgatt cattcccgtt ctcttgtcgg
     2761 cgtcttcgac gaccgcgaca cgcggatcat gggcgcggcc taccgcatgg cttgcgacat
     2821 gttggatgcg aatggcggcg acgcggcgga gcgctcgctg cggttcgaaa tcgccgccgc
     2881 gctgctcgaa gcggccgatg acggcttttc cgacgcgccg gctttggcgc aagcggccgt
     2941 cgacctcgtt ttcgtcgatg cgatcgagat cgactttagc tgagcctcgc gcaggcttgc
     3001 ttcacgcgcg acgcgcccgc tcccagcgat ggatcacttg aagctggctg cggattcggg
     3061 cgcggcggcg gcggcgcagc atctcgccga aaaatcggac caggcgggcg aacaggccgc
     3121 gcttcggcgc cggtcctctt ctttcg
//
LOCUS       DATGJW010000146         4910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1477059, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000146 DATGJW010000000
VERSION     DATGJW010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4910
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1477059"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(73..774)
                     /locus_tag="VKU03_04430"
     CDS             complement(73..774)
                     /locus_tag="VKU03_04430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70540.1"
                     /translation="MALSQSQRLRKAAQKAARRKEIVAQKRKAEAAALAAPEAPSDYA
                     KRAPIYACFVTEDLFSAGIGWVALARRMPMDSVAASFFMVDPWCLGVKDAFFRTMPID
                     AFETHMSFLLDFQEPIEMKPSSVRKLLHDAAAYAGSLGFAPDDGFAVAESLFGDIAMA
                     DETFTFGKDGKPHFVPGPSDSQARIRHVMETLAERGTEGVDFTIGTALDLFADDDESD
                     DDGEAEAAALPAPPP"
     gene            977..1993
                     /locus_tag="VKU03_04435"
     CDS             977..1993
                     /locus_tag="VKU03_04435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0009037 - tyrosine-based site-specific
                     recombinase activity [Evidence IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine recombinase XerC"
                     /protein_id="HLJ70541.1"
                     /translation="MKPSPEADLFLAALPAAADLAEKIAAWLRGLAGERDLSPKTVEA
                     YGRDLRQFLAFLAERNGAPPGVADFAGIAPADLRAFLARRRAADVQSRSLMRALAGLR
                     SFARFLERQGEARASAFFALRAPRVARPLPKPLSPADARAAAAIETRAGEAREPWIIV
                     RDAAVLALLYGAGLRIAEALSIRRRDAPVGAVDALTVIGKGRKQRTVPVIAPVRAAIE
                     DYLKACPYALKPDGPLFVGARGGALSPRIIQLAMARLRGALGLSDSATPHALRHSFAT
                     HLLARGGDLRTIQELLGHSSLSTTQVYTAVDSARLLEAYRSAHPRARAPQRPDLAAAK
                     SRAR"
     gene            complement(1951..2487)
                     /locus_tag="VKU03_04440"
     CDS             complement(1951..2487)
                     /locus_tag="VKU03_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70542.1"
                     /translation="MKEAPTEKSQRPKPALVLAAAVFLALAWVWEAFMRAGRWLVGLI
                     PWAAFKAWVVAWIDRLPIVIVVLIYGIPFLIVEPLKGVLVWVIATGHVFAGIVALILL
                     ETFGVGLLAVIFDLTRQRLLTLRWFAWCYDKVLIFHEFADRLVKPYKEAARRQWGAFR
                     EWARAYRARLFAAAKSGL"
     gene            2732..3235
                     /gene="pal"
                     /locus_tag="VKU03_04445"
     CDS             2732..3235
                     /gene="pal"
                     /locus_tag="VKU03_04445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019996538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-associated lipoprotein Pal"
                     /protein_id="HLJ70543.1"
                     /translation="MQGFMNARAFKFGAALAVGLALAACARGSQDDALNGASAAAAGA
                     PGSYQDFTANVGDRVFFDTDSSELSARAQATLDKQAAWLNRYNRYNFTIEGHADERGT
                     REYNFALGARRADTTKNYLIAKGVAASRIKTISYGKERPVAVCNDISCWSQNRRAVTV
                     LAGAPTS"
     gene            3343..4347
                     /gene="ybgF"
                     /locus_tag="VKU03_04450"
     CDS             3343..4347
                     /gene="ybgF"
                     /locus_tag="VKU03_04450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015952913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tol-pal system protein YbgF"
                     /protein_id="HLJ70544.1"
                     /translation="MKFDVYSARVGIVCATVLLFGAAPPTIAFAQSGLPPAGIPGAES
                     GDAAALVVRVERLEEELRAANGAIEELQNQQRRLQEQLKRFQEDVEFRFNGANGARPS
                     AAQSAPAPAAAPVVAAAPAATLETGAARPKRSDAFDPATEPGAVGAPKPLGSTAPSAP
                     LSGPGGAVGAPLDLSHPGAVAAQPTPTGPLSDADQPTVIAGISGPAVEGPKEQFAAAV
                     EAYRAGQYEQAEAQFRAFLARNSGSRLAPDATFYLGESYLQRSRPREAAEQYLKLSTD
                     YAKSPRAPEGMLRLGQSLAMLGNNEQACATFGEVGRRFPTAAASIKRSVEREMQKDHC
                     "
     gene            4344..>4910
                     /gene="tilS"
                     /locus_tag="VKU03_04455"
     CDS             4344..>4910
                     /gene="tilS"
                     /locus_tag="VKU03_04455"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLJ70545.1"
                     /translation="MRRKPSTAETIDAARIEALFAPLVDAKGLLVASSGGPDSTALLI
                     MAAQWAKAPGRPKVEVATVDHGLRPEARGEAEAVGKLARRRGLAHRILEWRGEKPKAR
                     IQERAREARYRLLAAEARAIGADHIVTAHHADDQCETILFRLLRGSGIAGLRGMAPFA
                     LLDGLAVARPLLALRKSELVTYCRAEGEA"
BASE COUNT          785 a         1738 c         1620 g          767 t
ORIGIN      
        1 aaaattgcgc gactacccga tgccgcccgc cgacgcgccg ctcatcccgc cgctgctcga
       61 tttgctcggc gtttagggcg gcggagccgg aagggcggcg gcttcggcct cgccgtcgtc
      121 atcggattcg tcatcgtcgg cgaacaggtc gagagccgtt ccgattgtga agtcgacccc
      181 ttccgtcccc cgctcagcca gggtctccat gacgtggcga atgcgggctt gagaatcgct
      241 cggccccggg acgaagtgcg gcttgccgtc cttgccgaat gtaaaggtct cgtccgccat
      301 ggcaatgtcg ccgaacagcg actcggcgac agcgaaacca tcgtccggcg caaagccgag
      361 cgacccggcg taggccgccg cgtcgtggag aagcttgcgg acgctggacg gcttcatctc
      421 gatcggctcc tgaaagtcga gaaggaagct catatgagtc tcgaaggcgt cgatcggcat
      481 ggttcggaag aaggcgtcct tgacgccgag gcaccatgga tcgaccatga agaaactcgc
      541 ggccacggaa tccatcggca tgcggcgcgc cagcgccacc cagccgatgc ccgcgctgaa
      601 caggtcttcg gtgacgaagc aggcgtaaat cggcgcgcgc ttggcgtagt cggacggcgc
      661 ctccggcgcg gccaaagccg cggcttcggc tttccgtttc tgcgccacga tctccttgcg
      721 gcgcgcggct ttttgcgcgg ctttgcgcaa tcgctggctt tgcgacaggg ccatgtctgc
      781 tcctcgcgaa cggggcgcga ctcgcgctca aaacgaatcg cgcgatccgg cgattttagc
      841 cccaggcgcg ggcggcgggc gcgccttgcc tgccgcgcag attgcttgcc cacacgattc
      901 tgggcgtgcg atcgaagcga atgactgcgc cgcgtcgcca acgctcggtt aagattcctc
      961 gcgcatcgta cccggcatga aaccctcgcc cgaggccgat cttttcctcg ccgccctgcc
     1021 ggcggcggcg gatctcgccg aaaaaatcgc cgcctggctt cgcggtctgg ccggcgagcg
     1081 cgatctgtcg ccgaaaacgg tcgaggccta tggccgcgat ctgcgccagt tcctcgcttt
     1141 cctcgccgag cgcaacggcg cgccgccggg cgtcgccgac ttcgcgggaa tcgcgccggc
     1201 cgatttgcgc gcctttctcg cgcgccgccg cgccgcggac gtgcagagcc gctcgctgat
     1261 gcgcgcgctg gcgggtttgc gctcgttcgc gcgctttctc gagcgccagg gcgaagcgcg
     1321 cgcctcggcc ttcttcgctt tgcgggcgcc gcgcgtcgcc cgccccttgc cgaagccgct
     1381 gagccccgcc gacgcgcgcg ccgccgccgc gattgaaacg cgggcgggcg aagcgcgcga
     1441 gccgtggatc atcgtccgcg acgccgcggt cctcgcgctg ctctacggcg ccggcctgcg
     1501 catcgccgag gctctatcga tccgccgccg cgacgcgccg gtcggcgcgg tcgacgcgct
     1561 gaccgtgatc ggcaagggcc gcaagcaacg caccgtcccg gtcatcgccc cggtgcgagc
     1621 ggcgatcgaa gattatctga aagcctgtcc ctatgccctg aagccggatg ggccgctctt
     1681 tgtcggcgcg cgcggcggcg cgctctcgcc gcggatcatc cagctcgcca tggcgcgcct
     1741 gcgcggcgcg ctcggtcttt ccgactcggc gacgccgcac gccttgcgcc attccttcgc
     1801 cacgcatctg ttggcgcggg gcggcgatct gcgcacgatc caggaattgc tcggccattc
     1861 ctcgctgtcg acgacgcaag tctataccgc ggtcgacagc gcccggctgt tggaggccta
     1921 ccgctcagcc catccgcggg cgcgcgcgcc tcaaaggccc gatttggccg cggcgaagag
     1981 ccgcgcgcgg taggcgcgcg cccattcccg gaacgccccc cattggcgcc gcgcggcctc
     2041 cttgtagggc ttgaccagcc ggtcggcgaa ttcgtggaag atcagcacct tatcatagca
     2101 ccaggcgaac cagcgcaagg tcagcaggcg ctggcgggtg agatcgaaaa tcaccgccag
     2161 cagcccgacg ccgaacgttt cgagcaggat cagggcgacg atgccggcaa agacatggcc
     2221 ggttgcgatc acccagacca gcaccccttt cagcggctcg acgatgagaa aagggattcc
     2281 gtagatcaag accacgatga cgatcggcaa ccgatcgatc cacgcgacca cccaggcctt
     2341 gaacgccgcc caggggataa gcccgaccag ccagcggccg gcccgcataa aagcttccca
     2401 gacccaggcg agggccagga aaacggccgc cgccaggacc agagccggct tcggccgctg
     2461 agacttctcc gttggcgctt ctttcatccg cgccataagg caggcctcta aggcgaattc
     2521 aagaacaaat cgcgacggtg aatttggccc tgcggcgccc tggcgcttgc gcggccgccg
     2581 caatgccgcc attaaccccg gcttaaccat cgcccgaatt ggcgagaatg cctcatatcg
     2641 cagaaacgcg tcggcaaggt tgacagaacc ttcctttaac cgttcgcgct taagcgtcgg
     2701 ccttaagggg cgtagggtca ggagtatcgt catgcagggg ttcatgaacg cgcgcgcgtt
     2761 caaattcggg gcggcgctcg ccgtcggcct ggctctcgcg gcctgcgcga gagggtcgca
     2821 ggacgacgcg ctcaacggcg cctcggcggc ggcggccggc gcgcctggct cctaccagga
     2881 tttcaccgcc aatgtcggcg accgggtctt cttcgacacg gattcttcgg aactctcggc
     2941 gcgcgcgcag gcgacgctcg acaagcaagc cgcgtggctc aaccgctata accgctacaa
     3001 tttcaccatc gagggccatg ccgacgagcg cggcacccgc gaatacaatt tcgccctcgg
     3061 cgcgcggcgc gccgatacga cgaagaacta cctgatcgcc aaaggcgtcg cggcgtcccg
     3121 catcaagacg atctcctatg gcaaggagcg gccggtggcg gtctgcaacg acatttcatg
     3181 ctggtcgcag aatcgtcgcg ccgtaaccgt gctcgccggc gcgccgacct cgtaaaagcc
     3241 caagcttcgc ccaaattttg gctgaacctt cggcgtcatg ctaggaccct gagcgcttcg
     3301 cgccaagcgc gccgaagcgc ggcattgtgg gggtcggctg ctttgaaatt cgacgtttat
     3361 tccgctcgcg tcggaatcgt ctgcgcgacg gtccttcttt tcggcgcagc gccgccgaca
     3421 attgctttcg cgcaatccgg cctgccgccg gcgggcattc cgggcgccga atccggcgat
     3481 gcggccgcgc tggtcgtacg ggttgaaagg ctggaagagg aattgcgcgc cgccaatggc
     3541 gcgatcgaag agctgcagaa tcaacagcgc cgcctgcagg agcagttgaa gcgcttccag
     3601 gaggatgtcg agttccgctt caacggcgcg aacggagcgc gtccctccgc cgcgcagagc
     3661 gcccccgcgc cggccgcggc cccggtcgtc gcggcggccc ccgccgcgac gctcgagacg
     3721 ggcgcggcga ggccgaagcg ctccgacgcg ttcgatccgg cgaccgagcc cggcgctgtc
     3781 ggcgcgccca agccgctcgg ctcgaccgcg ccgagcgccc cgctcagcgg cccgggcggc
     3841 gcggtcggcg cgccgctcga tctttcccat ccgggcgccg tcgccgccca gccgacgccg
     3901 accggcccgc tctcggacgc cgaccagccg acggtgatcg ccggcatttc agggccggcc
     3961 gtcgaagggc cgaaagagca gttcgccgcc gccgtcgaag cttatcgcgc cggccaatat
     4021 gagcaggcgg aggcgcagtt ccgcgccttc ctggcgcgca attcgggcag caggcttgcg
     4081 ccggacgcga ccttctacct cggcgagagc tatcttcagc gctcgcggcc gcgggaagcg
     4141 gccgagcaat atctcaagct ctcgaccgac tacgccaagt cgccgcgcgc gcccgaaggc
     4201 atgttgcggc tcggccagtc gctcgccatg ctcggcaata acgagcaggc ctgcgcgacg
     4261 ttcggcgaag tcggccggcg ctttccgacg gccgccgcct cgatcaagag aagcgtcgag
     4321 cgcgaaatgc agaaggatca ctgttgaggc gaaagccttc aacggcggag acgatcgacg
     4381 cggcgcggat cgaggcgctt ttcgcgcctc tcgttgacgc caagggcctg ctcgtcgcct
     4441 catccggcgg acccgactcg acggccttgc tgatcatggc ggcgcaatgg gccaaggcgc
     4501 ccggccggcc gaaggtcgaa gtcgccacgg tcgatcacgg cctgcgcccc gaagcgcgcg
     4561 gcgaggcgga agcggtcggc aaattggcgc ggcggcgcgg tctcgcccat cgcatcctcg
     4621 aatggcgcgg cgaaaagccg aaagcgcgca tccaggagcg cgcccgcgag gcgcgctacc
     4681 ggctgctcgc ggccgaggcg cgcgccatcg gggccgacca tatcgtcacc gcccatcatg
     4741 ccgacgacca gtgcgagacg atcctgtttc gcctgctgcg cggctcgggc attgccgggc
     4801 tgcgcggcat ggcccccttc gccttgctcg acggcctcgc cgtcgcgcgg cctttgctcg
     4861 ctttgcgcaa gagcgagctc gtcacctatt gccgcgccga gggcgaggcc
//
LOCUS       DATGJW010000147         5121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_26243, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000147 DATGJW010000000
VERSION     DATGJW010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26243"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..262)
                     /locus_tag="VKU03_04460"
     CDS             complement(<1..262)
                     /locus_tag="VKU03_04460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HLJ70546.1"
                     /translation="MLDPALHPELLNPAEMAAADRLAIAGGTPGIVLMEAAGKAVANE
                     AARMARSAGRIVVLCGPGANGGDGFIAARLLEARGYRVRTALL"
     gene            398..473
                     /locus_tag="VKU03_04465"
     tRNA            398..473
                     /locus_tag="VKU03_04465"
                     /product="tRNA-Asn"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:431..433,aa:Asn,seq:gtt)
     gene            complement(642..1028)
                     /locus_tag="VKU03_04470"
                     /pseudo
     CDS             complement(642..1028)
                     /locus_tag="VKU03_04470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003540945.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
     gene            complement(1025..1282)
                     /locus_tag="VKU03_04475"
     CDS             complement(1025..1282)
                     /locus_tag="VKU03_04475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799113.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLJ70547.1"
                     /translation="MQISVTEAKGQLTELIRRAEAGDEVILTRHGQPAVRLAPIKATP
                     DRKARRALLDVVRASAAVNATAGPSAARSQDFLYGDNGLPG"
     gene            1640..2620
                     /locus_tag="VKU03_04480"
     CDS             1640..2620
                     /locus_tag="VKU03_04480"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ70548.1"
                     /translation="MESITAGQLRAARGLLGLSQRDLAVLSKVSRATIADFEAGKRQP
                     YPRTLADLQRCLEGAGVAFTSDDDLLGVRIKRSHPPVSHAAGSPARPVRSAKPKEDGQ
                     IAQLRRRVLDRFAKAGVTPEQVKAGRELLGWSEFDLASKVGVSETAISLFEREKRRLL
                     MVDTSQLRAALEAAGVEFVHDGESGVRLKAKQPEITLEEFSSQLEWYEQQELRPKGIS
                     LGAKGGVKLGFALLYLDRSSAGLMLEGKELGRVRWSDGAVEFEPPIAGSDHARFEDRL
                     VLWASAAYAQSLAGSEPRPTHNIRQYAPRSDNLREAPQSAGSSWLKRGTP"
     gene            complement(2707..4002)
                     /locus_tag="VKU03_04485"
     CDS             complement(2707..4002)
                     /locus_tag="VKU03_04485"
                     /inference="COORDINATES: protein motif:HMM:NF021603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2336 domain-containing protein"
                     /protein_id="HLJ70549.1"
                     /translation="MAFVGVTRRAFAPSESEASNVRHFLAWARSASEEQRAEAAVTLA
                     WAYRYIADPDELHFDLELCIAAVLDDPSPAVRYALAEAIADVPDAPRHVVIALADDRP
                     DIAALVLAQSPALTEAELAHYAWTGEECVQAAIARRKDLPPLAAACLVEAGGVDPVMA
                     MIANRHARLPAAVFRRAAERFGAEPAVRNAMLQRPDLPAWIRCDLIEAAAAASASLLA
                     GSQIKPARIEKMVRSATNRSIVRAASGCSPEELRELIRHLRKKGTLTVALLMRALASG
                     EIAFFEEAAAELCGMDIRHVAALVRDPLGSGFAALIRRAGLPSYCLEPFAAALSALCD
                     LRIAGCGKAVRLIVLRVIQCCEQMPAPERAALLALLRSLEKEAALDDARMFAQDARER
                     LEERQKAGGLERSETPRCAHRVEQLNLIEMERPPLAMAC"
     gene            4181..4567
                     /locus_tag="VKU03_04490"
     CDS             4181..4567
                     /locus_tag="VKU03_04490"
                     /inference="COORDINATES: protein motif:HMM:NF013561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix domain-containing protein"
                     /protein_id="HLJ70550.1"
                     /translation="MPKVRLNVEISQQQADLLDALAEAENATRSEMVRRAIYVLQAFQ
                     QQQALGRPHLGFVRDAQRLDAEILGILQAPPGGAPPAAPPHIAKPEVERIAAPPLLTS
                     TAGGSGPLSMEAAMARWSPHLGGRAD"
     gene            complement(4942..>5121)
                     /locus_tag="VKU03_04495"
     CDS             complement(4942..>5121)
                     /locus_tag="VKU03_04495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLJ70551.1"
                     /translation="VAIVSAVKGKLGERVVLYLDRIGRLEGTVARHFGGGFAVALRAG
                     ALKREALASTLEKLS"
BASE COUNT          920 a         1710 c         1633 g          858 t
ORIGIN      
        1 cgagcagagc cgttctgacc cgatagcccc gcgcttcgag aagccgcgcc gcgatgaagc
       61 cgtcgccgcc attggcgccc ggcccgcaca gcacgacgat gcggccggcc gagcgcgcca
      121 tccgcgccgc ctcgttcgcg actgctttgc cggcggcctc catcaggacg attcccggcg
      181 ttccgccggc aatcgcaagg cggtcggcgg cggccatttc ggccggattg agcaattcgg
      241 gatggagggc cggatcaagc atctcgccga tcgtggcgca gcggcgcagc gcgcgcaacg
      301 cgcttccctc atgccgcgcg cgccgccgag cccgccgagg gattttaggc gcttttcaac
      361 cggcgaaatc gtgctatatg cgcgccaccc ggcctgatcc ccgatagctc agctggtaga
      421 gcattcgact gttaatcgaa tggtcgcagg ttcgagtcct gctcggggag ccacgggcgc
      481 cccggcgctt tcaatcgctg acgccttccc gtccttcgac gctcatcaac ggttgcggtc
      541 attcaggcga ccagtttctg cgcatatcag gttctgaggc tcttgaaact ttcgcgtgca
      601 gtttctagcc cagcggcagc caaaaacgcc gaagggctac gttagccgag agccctttcg
      661 acatcggtcc ggctaaaatc ctctccgaca tagagcaggc ggcagccacg ctctttcgct
      721 acttcataag cgaagcaatc gccgaaattc agcgctgcgg gatgaacgcc ttttcctcat
      781 tgttcgtagg cgcgtgcgat gcgccgcgcc gccgcggggg tcacgctgac gacctcaaaa
      841 ccgagccccg caatgagacg ctccatctcc tcgccgacat gtcgccgacc agcgacgatc
      901 aacgcctcgg ccaccgttcc ggcggagatc aggagatcgt catcgccctc caacgctgcg
      961 gcacaggcat tcgcttccgt ctcgttaagc aggatcgcca tgagcgccga cgtgtcgacc
     1021 gctatcatcc gggcaggccg ttatcgccgt agaggaaatc ctggctgcgc gctgcgcttg
     1081 gaccggccgt cgcgttcacg gcggcggagg ctcggaccac atcgagaagc gcgcggcgcg
     1141 ccttccggtc cggcgtcgcc ttgatcggcg cgagccgcac ggcgggctgg ccgtgtcggg
     1201 tcaatatgac ctcatcgcca gcctcggccc gccggatcaa ctcggtcaac tgtccttttg
     1261 cttctgtgac ggatatctgc atgactcgtc ctgatttggt cacgtcaaaa accccgctca
     1321 agaataattt agaccatcag atagtccgtt acaattccgg cagccgcgag agcggtcgga
     1381 attgcatctc gttctcgggc tattggcgac gtcaggcgat tcgcccctcc ggacgcaaca
     1441 gaatttccgc gtgttcggcg gccaaccctg gccagcgaat ttgcatagcc tgtcggcacc
     1501 ccgtcagcga tgaacagtgt cgcagcgatt cctcataatc cattaaccgt acgcaaacgc
     1561 tgcgtcgcgc aatcatgccg gggtcaggcc aaatgtggta ctttgcgcca aaagctcgaa
     1621 gttggactgg gtgggccgca tggaatcgat taccgcaggt cagctccgcg cggcgcgcgg
     1681 cctgctcggc ttgtcgcagc gagaccttgc cgttctctcg aaagtcagcc gcgcgacgat
     1741 cgccgatttc gaagccggca agcgccagcc ctatccgagg acgttagccg atctgcagcg
     1801 ctgcttggaa ggggccggcg tcgctttcac gagcgacgac gacctgctcg gcgttcggat
     1861 caagcgctct catccgccgg tttcgcacgc cgccggcagc ccggctcggc ctgtcaggtc
     1921 cgccaagccg aaagaggatg ggcagatcgc ccagcttcgg cgccgggttc tcgaccgctt
     1981 cgccaaagcc ggcgtgacgc ccgaacaggt caaagcgggc cgagaactcc tcggctggtc
     2041 ggaattcgac cttgcgagca aggtcggcgt cagcgaaacc gcgatcagcc tgttcgagcg
     2101 ggaaaagcgc cggttgctga tggtcgatac gtcgcaattg cgcgccgcgc tggaggcggc
     2161 aggcgtcgaa ttcgtccatg acggcgaatc gggggtgaga ttgaaggcga agcagccgga
     2221 aatcacgctc gaagagtttt ccagccagct cgaatggtac gagcagcaag aattgcggcc
     2281 caaagggatc agcctcggcg ccaaaggcgg cgtgaagctg ggcttcgccc tcctctattt
     2341 ggaccgcagc tcggccggct tgatgctgga aggcaaagag ctggggcgcg tcaggtggag
     2401 cgatggcgcc gtcgagttcg agccgccgat tgccggatcg gaccacgccc gcttcgagga
     2461 tcggctcgta ctatgggcga gcgccgccta tgcgcaaagc ctggccggca gcgagccgag
     2521 gccgacgcac aacattcgcc aatatgcgcc aaggtcagac aatttgcgcg aggcgccgca
     2581 atctgccgga tcgtcctggc tgaagcgcgg aacgccgtga acgccagccg agcccccgtc
     2641 ctttaacggc gcgcggcggc gaacatctct ggacagggtc gcgccaggcc gcgtcttatt
     2701 ttccgctcag caggccatgg ccagaggcgg ccgctccatc tcaatcaggt tgagttgctc
     2761 aacacggtga gcgcagcgcg gcgtttcaga gcgctcaagg ccgccggcct tctggcgctc
     2821 ttcgaggcgc tccctcgcat cctgcgcgaa catccgtgcg tcgtcgagcg ccgcttcttt
     2881 ttcgagactt cgcaagagcg cgagaagcgc cgctctttcg ggcgccggca tttgctcgca
     2941 gcactgaatc acgcggagaa cgataagccg cacggccttt ccgcaaccgg cgattctcaa
     3001 atcgcataga gccgaaagag cggcggcgaa cggctctagg caataagaag gcagccccgc
     3061 ccgcctgatc agcgccgcaa atccagagcc cagcggatca cgaaccagcg ccgcgacgtg
     3121 gcgaatatcc atgccgcaga gctccgccgc cgcttcttcg aagaaagcga tttcgccgga
     3181 ggccaaggcg cgcatgagca gcgcgacggt cagcgtccct tttttgcgaa gatgacggat
     3241 cagctcgcgg agctcttccg gcgaacaccc gctggcggcg cgaacaatgc tccggttcgt
     3301 cgccgagcgg accattttct cgatccgagc cggcttgatc tgcgaaccgg caaggagcga
     3361 ggcgctggcc gccgcggccg cttcgatcag gtcgcaacga atccaggctg gcagatcggg
     3421 ccgctgcaac atcgcgtttc gaaccgcagg ctcggcgccg aaccgctccg ccgcccggcg
     3481 aaagaccgcc gccggcaagc gcgcatgccg gttcgcgatc atcgccatca ccgggtcgac
     3541 gccgcccgcc tcgaccaggc aggcggccgc gagcggcggc aggtccttgc gccgcgcgat
     3601 cgccgcctgg acgcattcct ccccggtcca ggcgtaatgg gcgagttcgg cctcggtcag
     3661 cgccggcgac tgggcgagga cgagggcggc gatgtccggg cgatcgtccg ccagcgcgat
     3721 gacgacatgc ctcggcgcgt cgggaacatc ggcgatcgct tccgcaagcg cgtatcgaac
     3781 cgcgggcgac ggatcgtcga ggaccgcggc gatgcaaagc tccaaatcga aatggagttc
     3841 gtcgggatcg gcaatatagc gataggccca ggccagcgtg acggcggctt cggcgcgctg
     3901 ctcctcgctc gccgagcgcg cccaagccag gaaatggcgg acattggacg cctcagactc
     3961 cgagggcgca aaggcgcgcc gagttacgcc aacgaacgcc acgcaaaaac cagaacgctt
     4021 ccaaggacaa cgacgttctt gctagcagca attgattgac caaacgtcac tgcgggcgcc
     4081 gcgcctaaag ttttaccgaa ttgggaaacg gaatcggccg gcgccgcatg ttacgccttc
     4141 tataggcccg gctgcttcgg gtgctggaga cgacggtcgg atgccgaagg ttcgcttgaa
     4201 cgtcgaaatc agccagcagc aagcggacct cctcgacgcc ctggcggaag cggaaaacgc
     4261 gacccgctcg gaaatggtgc ggcgcgcgat ttacgtgctg caagccttcc agcagcagca
     4321 ggcgctcggc cgtccgcatc tcggcttcgt cagagacgcg caacggctgg acgccgaaat
     4381 cctgggcatc ctccaggcgc cgccgggcgg cgcgccgccc gctgcgccgc cgcatattgc
     4441 gaagcccgag gtcgagagga tcgccgcccc gcccctcctc acctccaccg ccggcggatc
     4501 gggtcccctc tcgatggagg ctgcgatggc gcgctggtct ccgcatctgg gcggccgcgc
     4561 cgactgacgg tccccgcttg gccgcatcgc tgcggccggc ttctcatccc gcaagcccga
     4621 aagagcgcct gagctgcgcc ccgcgtttct gcgtcccgcg gcttggccgc ggcgattgcg
     4681 cccctcgcgc agcccgcgac ttttggcctg agatcagact tcgcgcctga ccgtcgcgcc
     4741 agagatcaga cttttggctt tgggtcagcg cgatctcgca gcggatgagc gttttttgct
     4801 catgcgattg gaacggggtc ggtcggggtt tacaggcgcg ccggctgtcg ggcgacgggt
     4861 ggaaaatgcc gggcaagcgc gtatccgctc tcgccggaac gtggcgagcg aaaaagcggc
     4921 gcggttagaa cgcggccgat ctcagctcag cttttccagg gtcgaggcga gcgcttcacg
     4981 cttgagcgcg ccggcgcgga gcgcgacggc gaaaccgccg ccgaaatggc gcgcgaccgt
     5041 tccttcgagt cttccaatac gatcgagata gaggacgacg cgctcgccga gcttgccctt
     5101 gactgcgctc acaatggcga c
//
LOCUS       DATGJW010000148         2764 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_440674, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000148 DATGJW010000000
VERSION     DATGJW010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2764)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2764)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2764
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_440674"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1542
                     /locus_tag="VKU03_04500"
     CDS             <1..1542
                     /locus_tag="VKU03_04500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl CoA:acetate/3-ketoacid CoA transferase"
                     /protein_id="HLJ70552.1"
                     /translation="SSGLGCPEAMLAAIGRRFEAEGHPRNITTLHPIAVGDMWGIAGI
                     DHLAKDGCLARILAGSYPSGPSRAEPPKIWRMVSDNRIPAYNIPSGILFDMHREAAAK
                     RPGVLTKIGMDTFVDPAREGCAMNAKAAATPIVSKVQFAGDEWLFFPAIIPDVAIIRA
                     TTADERGNLSYEHEGGYLGPLDQALAVRNNGGIVIAQVKRLAQAGTQKPQHVLVPGIL
                     VDAIVVAPDQMQTTQTIYEPAISGEIFRPLSSFDIPEFGVGKVIARRVAQELRHGDAV
                     NIGFGISANVPRILIEEGQHGAVTWVIEQGAVGGVPLLDFQFGCASNAEAIMPSPYQF
                     TYFQGAGFDCSLLSFLEIDRQGSVNVSKLGARPHVTAGAGGFVDITARAKKIVFSGYF
                     TAGAGLAVTDGALVIEKEGKVKKLVEEVEQVSFSGARAKAQGQDIVYVTERCVLRLMR
                     EGVTVVEIAPGVDLERDILAQAHFPLRVAADLKTMDRALFHPEPIGLDLQVAPARLAN
                     REAAE"
     gene            1539..2312
                     /locus_tag="VKU03_04505"
     CDS             1539..2312
                     /locus_tag="VKU03_04505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004686221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLJ70553.1"
                     /translation="MSEHIRLSLDGPIARITVTRAEKLNALDRAMIVALAEAATAIEA
                     SKEVRVAILSGEGKAFCAGGDIAAWGGLPPLEMWRDWTRIGHRAFEALARLRVPLICA
                     LTGHAFGGGLELAAVADIRVAERGIKLGLPETGLAMAPGWSGTQRLVRRFGAGVVRRM
                     ALSGAMFTADEALALGLVDEVVEAGAGLARAAAIAAGIAARGPLAVQMVKAMINAAEG
                     EDKDAPIEGLAGALTAMTEDLAEGVAAFRGKRPAAFVGR"
     gene            2484..>2764
                     /locus_tag="VKU03_04510"
     CDS             2484..>2764
                     /locus_tag="VKU03_04510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ70554.1"
                     /translation="MAAGFAGAIAVAHAADNKEVQMLHWWTSGGEAAALNVLKQDLSK
                     EGYAWKDVPIAGGGGEGAMTALKAMVAAGNPPTASQILGYFAIDYAEAG"
BASE COUNT          487 a          910 c          871 g          496 t
ORIGIN      
        1 tcctcggggc ttggttgtcc tgaagccatg ctggccgcaa tcggccggcg cttcgaggcg
       61 gaaggtcatc cgcgcaacat cacgactttg cacccgatcg ccgtcggcga catgtggggc
      121 atcgccggga tcgaccatct cgccaaagac ggctgcctcg cccgcattct cgctggctcg
      181 tacccgtccg gtccgtccag ggcagagccg ccgaaaatct ggcgcatggt ctcagacaac
      241 cgcatccccg cctacaacat cccgtccggc attctcttcg acatgcatcg cgaggcggcg
      301 gcgaagcgcc caggcgtctt gactaagatc ggcatggata ctttcgtcga tcccgcgcgc
      361 gaaggctgcg ccatgaatgc gaaagcggct gcaacgccga tcgtcagcaa ggttcaattc
      421 gccggcgacg aatggctctt cttccccgcg atcattcccg atgtcgcgat cattcgcgcg
      481 accaccgccg atgaacgcgg caatctttcc tatgaacatg aaggcggcta tctcggcccg
      541 ctcgatcaag cgctcgccgt tcgcaacaat ggcggcatcg tcatcgccca ggtcaagcgc
      601 ctcgcccagg ctggcacgca aaagccgcag catgtcctcg ttcctggcat tctcgtcgac
      661 gcaattgtcg tcgcgcccga tcagatgcaa acgacgcaaa cgatctatga gccggcgatt
      721 tccggcgaga tattccggcc gctctcttcg tttgatatcc ctgaattcgg ggtcggcaaa
      781 gtcatcgccc gccgcgtcgc gcaagagctc cgccacggcg acgcggtcaa tatcggcttc
      841 ggcatttccg ccaatgtgcc gcgcattctg atcgaagaag gtcaacacgg cgccgtcacc
      901 tgggtcatcg agcaaggcgc agtcggcggc gtgcctttgc tcgacttcca attcggctgc
      961 gcctcaaacg ctgaagccat catgccttcg ccgtaccaat tcacctactt tcagggcgcg
     1021 ggcttcgatt gctcgctcct gtcctttctc gagatcgatc gccaaggttc agtcaacgtc
     1081 tcgaaactcg gcgccagacc gcatgtcacc gccggcgccg gcggctttgt cgacatcacg
     1141 gcgcgggcga agaagatcgt cttctcaggc tatttcaccg ccggcgccgg cctcgccgtc
     1201 accgatggcg cgctcgtcat cgagaaggaa ggcaaggtca agaagctggt cgaagaggtc
     1261 gaacaagtct ccttctccgg cgccagggcc aaggctcaag gccaggatat tgtctatgtc
     1321 accgagcgtt gcgtcttgcg cttgatgcgc gaaggcgtga ctgtcgtcga gatcgcgccg
     1381 ggcgtcgacc tggaacgcga cattctcgcg caggcgcatt ttccgctgcg cgtcgccgcc
     1441 gatctcaaga cgatggatcg cgccttgttc caccccgagc cgatcgggct cgacctgcag
     1501 gtcgcgccgg cccggctggc caatcgcgag gcggcggaat gagtgaacat atacgcctct
     1561 ccttggacgg accgatcgcg cgaatcacgg tgacccgggc cgagaagctc aatgcgcttg
     1621 atcgcgccat gatcgtcgcg ctcgccgagg cggctacagc gatcgaagct tcgaaggaag
     1681 tacgagtggc gatcctctcc ggcgaaggta aagctttctg cgccggcggc gacatcgccg
     1741 cttggggggg cctgccgccg ctcgaaatgt ggcgcgattg gacccgcatc ggccatcgcg
     1801 ctttcgaggc cttggcgcgc ctccgcgttc ccctgatttg cgccctgaca ggccacgctt
     1861 tcggcggcgg tctggagctc gccgccgtcg ccgacatccg cgtcgccgag cgcggcataa
     1921 aactgggcct tcccgagacc ggtctcgcca tggcgcccgg ctggtccggt acgcagcgtc
     1981 tcgtgcggcg tttcggcgcc ggcgtcgtac gccgcatggc gttgagcggc gcgatgttca
     2041 cggcagatga ggcgctcgcc ctcggactgg tcgatgaagt cgtcgaagcc ggcgccggtc
     2101 tcgcccgtgc ggccgcgatc gccgcgggca tcgccgcccg cggcccgctc gcggtgcaaa
     2161 tggtcaaagc gatgatcaat gccgccgaag gcgaggacaa agacgcgccg atagaaggcc
     2221 tcgctggcgc gctgaccgcg atgacggagg accttgccga aggcgtcgcg gcgtttcgcg
     2281 gcaaacgtcc ggccgccttc gttggtcggt gagagaatga gctttccacg tcaacaatgg
     2341 cggcaagccg ctggggcgtg gttggagaga cagggaccgc atttccggtt cttggaaacg
     2401 gcgccggacc gaatccggtt gggatattag gctgattgga agggttggac atgaaacttc
     2461 gtaccaagct cctcgcgggc gcgatggcgg cgggcttcgc cggcgcgatc gccgtcgctc
     2521 acgctgccga caacaaagaa gtgcagatgc tgcattggtg gacgtcaggc ggcgaagcgg
     2581 ccgcgttaaa cgtgctcaag caggacctct ccaaggaagg ctacgcctgg aaggacgtgc
     2641 cgatcgcagg cggcggcggc gaaggcgcca tgaccgcgct gaaggcgatg gtcgccgccg
     2701 gcaatccacc gaccgcctcg caaatcctcg gctatttcgc catcgactac gccgaggccg
     2761 gcaa
//
LOCUS       DATGJW010000149         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1065169, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000149 DATGJW010000000
VERSION     DATGJW010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1065169"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..297)
                     /locus_tag="VKU03_04515"
     CDS             complement(<1..297)
                     /locus_tag="VKU03_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70555.1"
                     /translation="MNEANPSFLRQTVKPPLSLHIPEPKFRPGQKVDFSGLRISEPGE
                     IRRPPIDVAPRDIRDLADGVIRVMNDRGEAVGPWDPKLSAEVARRALRYMMTTRA"
     gene            545..1918
                     /locus_tag="VKU03_04520"
     CDS             545..1918
                     /locus_tag="VKU03_04520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLJ70556.1"
                     /translation="MAAAENCAPTPGLSAFLAEIGDVPTIADLQVVRRRSRDFFWYSP
                     ILNAQLHGKSAEIVVTPRHEADVIAVARAAARHGVPLTPRGGGTGNYGQAVPLKGGAL
                     VDFTAMNHILWNRPGVVRVQAGAKMGAIDEALQPFGWELRLHPSTKRTATIGGFVAGG
                     SSGIGSVAYGGLRDPGNIGAARIVTIEREPRVIELRADAAQKVNRAFGTTGLITELEM
                     PLSPAWRWMDVIVAFDDFMTAVETGRAIAMADGIVKKLATPIAWPIPAAFADIKAHCP
                     DDKSILIAMIAEPSLEAFRALIGKGVVTHEAPTDESAGKVPLYEYCWNHTTLHMLKGD
                     RSITYLQCLFPHDRLIATVEKMVAAFGDEVPHHLEFSRYAGHLTASALPIVRYKSPER
                     LSEIIAAYEANGVFVANPHVYTLEDGSRYRRADADQFGFKAEVDPYGLLNPGKMKSFV
                     GKPWAPA"
BASE COUNT          354 a          672 c          638 g          337 t
ORIGIN      
        1 agcgcgggtg gtcatcatgt agcgcaaggc gcggcgggcg acctcggcgc tcagcttcgg
       61 gtcccagggg ccgaccgcct cgccccgatc gttcatcacc cggatgacgc cgtcggcgag
      121 gtcgcggatg tcccgcggcg cgacatcgat cggcggccgg cggatttcgc ctggctcgga
      181 gatgcgcaga ccgctgaaat ccaccttctg gccgggccgg aatttcggct cggggatatg
      241 caggctgagc ggcggcttga cggtttgccg gagaaaagaa ggattggcct cgttcacgcg
      301 tttttcctcc gcgccgggct atcttggcgg cttgagccta aacgggaacg accgccccgt
      361 cgattctcgc cgcagcccta taaactgttt tggccgcctt cgcgttccca aggttgcgcg
      421 gcgccggaag ctcgtccacc atccctcctg tcattcccgc gcaagcggaa tccaggcagc
      481 caggacttca gttccggatc ccggcttgcg tgggaatgac ctcgctgagg aaacggagca
      541 agaaatggcg gcggccgaaa attgcgcgcc aacccccggc ctgtccgcct tcctcgccga
      601 gatcggcgac gttccgacca tcgccgacct gcaagtcgtg cgccgccgct cgcgcgattt
      661 cttctggtac tcgccgatcc tcaacgcgca gttgcatggc aaatcggccg aaatcgttgt
      721 gacgccacgc cacgaggcgg atgtgatcgc cgtcgcccgc gccgcggcgc gccatggcgt
      781 gccgctgacg ccgcgcggcg gcggcaccgg caattacggc caggcggtgc cgctcaaagg
      841 cggcgccctg gtcgatttca ccgcgatgaa ccacattctc tggaaccggc ccggcgtcgt
      901 gcgcgtccag gccggcgcga aaatgggcgc gatcgacgag gcgctgcaac ctttcggctg
      961 ggagctgcgc ctgcatccct cgaccaagcg cacggcgacg atcggcggct tcgtcgccgg
     1021 cggctcgagc gggatcggct cggtcgccta tggcggcttg cgcgatcccg gcaatatcgg
     1081 ggcggcgcgc atcgtcacga tcgagcgcga gccgcgcgtc atcgaattgc gcgccgacgc
     1141 ggcgcagaag gtcaatcgcg ccttcggcac gaccggcctg atcaccgagc tggaaatgcc
     1201 gctcagtccc gcttggcgct ggatggacgt catcgtcgcc ttcgacgatt tcatgacggc
     1261 ggtcgagacc ggccgcgcga tcgcgatggc cgacggaatc gtcaagaagc tggcgacgcc
     1321 gatcgcttgg ccgatcccgg ccgctttcgc cgacatcaag gcgcattgcc ccgacgacaa
     1381 aagcatcctc atcgcgatga tcgccgagcc gtcgctggag gcgttccgcg ccttgatcgg
     1441 caagggcgtc gtcactcacg aagcgccgac cgacgagagc gccggcaagg tcccgctcta
     1501 tgaatattgc tggaaccata cgacgttgca tatgctcaaa ggcgatcgct cgatcaccta
     1561 tctgcaatgc ctgtttccgc atgacagatt gatcgccacc gtcgagaaga tggtcgcggc
     1621 gttcggcgac gaagtcccgc atcatctcga attctcgcgc tatgccggcc acctcaccgc
     1681 cagcgccctg ccgatcgtgc gctacaaaag ccccgagcgg ctcagcgaga tcatcgccgc
     1741 gtacgaagcg aacggcgttt tcgtcgccaa tccgcatgtc tacacgctcg aggacggcag
     1801 ccgctatcgg agagccgacg ccgaccagtt cggcttcaag gcggaggtcg atccctacgg
     1861 cctgctcaat cccggcaaaa tgaagagctt cgtcggcaag ccgtgggcgc cggcgtgaaa
     1921 cggaggaacg atgccaaaag ccaatgaaaa cctggtcgtg atcggcggct cgtctggaat
     1981 aggcctgcag atcgccaaag a
//
LOCUS       DATGJW010000150         3689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_339643, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000150 DATGJW010000000
VERSION     DATGJW010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3689
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_339643"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1215)
                     /locus_tag="VKU03_04525"
     CDS             complement(97..1215)
                     /locus_tag="VKU03_04525"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HLJ70557.1"
                     /translation="MPKKPPFRADHVGSYLRPKRLAEARESFFAGKLPADALRQIEDE
                     CIRDVVRKQEDVGLKGITDGEFRRTYFHVDFLEKLDGVTVTYGEFVAKFRKDDGTEVG
                     FRPPTMHVGSKIRHATSIQGRDFDFLKSVTTRTPKVCIPAPSMLHFRGGRKAISEQAY
                     PDLEAFYDDLTAAYRDEIAGLAKRGCRYLQLDDTNLAYLCDPAIRESTKARGDDPDAL
                     TRLYCRLVNDSIKDRPADMVISVHLCRGNFKSAWVAQGGYEPVAEILLNEMKIDGFFL
                     EYDDERSGDFAPLRFAPKGKMIVLGLMSSKNRAVEPKDEIKKRIDEASRYVPLDQCGL
                     SHQCGFSSTAHGNDLSEGDEWRKLARCVEIAREVWGEV"
     gene            1445..1735
                     /locus_tag="VKU03_04530"
     CDS             1445..1735
                     /locus_tag="VKU03_04530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70558.1"
                     /translation="MSRAGEAKQQATPERTRRIGALVEIMRDCWDTPADCNAGELFAY
                     AEILLDRIEAGEGNEELDRYLADVQTEKLGVPASDAYRNIRVRALALAAKGR"
     gene            1888..2244
                     /locus_tag="VKU03_04535"
     CDS             1888..2244
                     /locus_tag="VKU03_04535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70559.1"
                     /translation="MLAREIIQTCTNVHVARAALISIGGDFAAQVAAKARETDRALGA
                     FVADAVNDFSRHAGDDEWDGVDEAARGSDQPILSGLRHILGVRLGIAAGAAFTVDPLC
                     ASSWTRSGFARGACLQ"
     gene            2342..3106
                     /locus_tag="VKU03_04540"
     CDS             2342..3106
                     /locus_tag="VKU03_04540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLJ70560.1"
                     /translation="MGFAVLGALFVVVAPAWAQSSRLDDIIARGVVKVGTTGDYRPFT
                     FLDKASGGYSGLDIDLAQSLAAALGVKVEFVATSWPHLAKDFDAGAFDIAMGGVSITL
                     ERAKKGFFSIPYLREGKTPIARCADKDKYQTLAEIDRPDVTVIVNPGGTNEKFDRAHL
                     HAARIETYPDNAAIFDQLAAGKADLMITDASETRYQQKLHKGALCAIHPDKPFDFAEK
                     AYWMPRDEPLKAFVDEWLHMNIENGALAAETAKWLE"
     gene            3276..>3689
                     /locus_tag="VKU03_04545"
     CDS             3276..>3689
                     /locus_tag="VKU03_04545"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HLJ70561.1"
                     /translation="MTYLGLDCGTSALKAALVDEDERVLATAVEAYQPVHPRPLWSEQ
                     NPDDWLAAMFAALARLARDRPEAMARVAAIGFSGQMHSAVLLDGADKPLRPAILHNDT
                     RAFAEAARLATRHAELIARVGVKPMAGFTAPKLMWL"
BASE COUNT          604 a         1170 c         1252 g          663 t
ORIGIN      
        1 cgtgttcgct ccggcgcagc atagcgggcg aaggcgcggc gcggcaaatg tgtcattccc
       61 gcgctgtcgc gggaatgacg gttctgaggg cgaacgctaa acctcgcccc agacctcgcg
      121 cgcgatctcg acgcaacgcg ccagcttccg ccactcgtcg ccctcgctga ggtcgttgcc
      181 atgggcggtc gaggagaagc cgcattgatg gctgaggccg cattgatcga gcggcacgta
      241 gcggctcgct tcgtcgatcc gcttcttgat ttcgtccttc ggctcgacgg cgcggttttt
      301 cgagctcatc aggccgagca cgatcatctt gcctttcggc gcgaagcgca gcggcgcgaa
      361 atcgccggag cgctcgtcgt catattcgag gaagaagccg tcgatcttca tttcgttgag
      421 caggatttcg gcgaccggct catagccgcc ctgcgccacc caggcgctct tgaaattgcc
      481 gcggcagaga tggaccgaaa tcaccatgtc ggcgggacgg tccttgatcg aatcgttgac
      541 gagccggcaa tagaggcggg tcagcgcgtc gggatcgtcg ccgcgcgcct tggtcgattc
      601 gcggatcgcc ggatcgcaga gataggcaag attggtgtcg tcgagctgca gatagcggca
      661 gccgcgcttg gcgaggccgg cgatctcgtc gcgataggcc gcggtgagat cgtcatagaa
      721 cgcttcgaga tcgggatagg cctgctcgct gatcgccttg cggccgccgc ggaaatgcag
      781 catggacggc gccggaatgc agactttcgg cgtgcgtgtc gtcacgctct tgagaaagtc
      841 gaaatcgcgc ccctggattg acgtcgcgtg gcggattttc gacccgacat gcatggtcgg
      901 cggccggaag ccgacttcgg tcccgtcgtc cttgcggaac ttggcgacga attcgccata
      961 ggtgacggtg acgccgtcga gcttttcaag aaagtcgaca tggaaatagg tgcggcggaa
     1021 ctcgccgtcc gtgatccctt taaggccgac atcctcctgc ttcctgacca cgtcgcggat
     1081 gcattcgtcc tcgatttggc gcagcgcgtc ggcgggcagc ttgccggcga agaaggattc
     1141 gcgcgcctcg gcgaggcgct tcgggcgaag ataggagccg acatgatcgg cgcggaacgg
     1201 cggcttcttg ggcattcgtc tcctccttgc cgccgcggcg cttgggcggg gcggcccttc
     1261 cagatttgcc gctctttcta gaggacggcg gggttttggc aaccgccggc gctttccggt
     1321 ccgcaaaggg gcgctggcgc cggcggcggc ggtgacgagc ggagcggcca tcttcccgaa
     1381 accggcaaat cgcgcgataa ggaactttcc gaacccgcgg cgcttggagg cgcggagact
     1441 gacgatgagc cgagccgggg aagcaaagca gcaagccacg ccggagagga cgcgacggat
     1501 cggcgccctt gtcgagatca tgcgcgattg ttgggatacg ccggctgatt gcaacgcggg
     1561 cgagttgttc gcctatgccg aaattctgct cgaccgcatc gaggcgggcg aaggaaacga
     1621 ggaactcgat cgttaccttg ccgacgtgca gaccgagaaa ctgggcgtgc cggcttccga
     1681 cgcctatcgc aatatccgtg ttcgcgcgct ggcgctggcc gcaaagggtc gctgactctt
     1741 cgtgatatgc tagcagcgca gtggcgtacg gcgcgctgtt cgcgctgtca caaggaagtc
     1801 tttttctccc gttaggtagg aggcgaaccg agtagaaccg gttcgcgcgt tgcgcgtttg
     1861 cgccgggttt gccgcaggga ggccggaatg ctcgcccgtg aaatcattca aacctgcacg
     1921 aacgtccatg tcgcccgcgc cgcgctgatc tcgatcggcg gcgattttgc ggcgcaggtg
     1981 gcggccaagg cgcgcgaaac ggatcgggcg ctcggcgcct tcgtagccga cgcggtgaac
     2041 gatttttcgc gccatgccgg cgacgacgaa tgggacggcg tcgacgaagc ggcgcgcggc
     2101 tccgatcagc caatattgtc cggcctgcgc catattctcg gcgttcggct tggcatcgcc
     2161 gccggcgccg ctttcacggt cgatccgctc tgcgcctcgt cctggacgcg cagcggtttc
     2221 gcgcgcggcg cttgcttgca gtgatcgcag cggcgcgccg ccttgccggc gcagccgact
     2281 tcgcgcaaca tggcgcccgc gcgcctggcg gcaggctgcg ggagggtcgg gcttgcggcg
     2341 catcggtttt gcggttctcg gcgcgttgtt cgttgtcgtc gcgccggcct gggcgcaaag
     2401 ctcgcgcctc gacgacatca tcgcgcgcgg cgtggttaag gtcggcacga ctggcgatta
     2461 ccggcctttc accttcctcg ataaggcgag cggcggctat tcgggcctcg acatcgatct
     2521 cgcccaatcg ctcgccgcgg cgctcggcgt caaggtcgag ttcgtcgcca cgagttggcc
     2581 ccatctcgcc aaggatttcg acgccggcgc tttcgacatc gccatgggcg gcgtctcgat
     2641 caccttggag cgcgccaaga agggcttttt ctcgattccc tatttgcgcg agggcaagac
     2701 gccgatcgcc cgctgcgccg ataaggacaa gtaccaaacc ctggccgaga tcgaccggcc
     2761 ggatgtgacg gtgatcgtca atccaggcgg gacgaacgag aaattcgacc gggcccatct
     2821 ccacgccgcg cggatcgaga cctatccgga taacgctgcg attttcgacc agctcgccgc
     2881 cggcaaggcc gatctgatga tcaccgacgc ctcggagacg cgctatcagc agaagctgca
     2941 taaaggcgcg ctctgcgcga tccatccgga caagcccttc gatttcgccg agaaagccta
     3001 ttggatgccg cgcgacgagc cgctgaaagc cttcgtcgac gagtggctgc atatgaatat
     3061 cgagaatggc gcgctggcgg ccgagacggc gaaatggctg gaatgatcgc gaagtacgaa
     3121 tcctcccccg cgaagcgggg gcgggggacc ggccgaaggc cggtggaggg ggccgcggcg
     3181 ctggcgacta ttctcaaccg gcgcgccccc gccaccgctc tgcgcgcggt ccccctcccc
     3241 cgcttcgcgg gggagggaaa gggcgacgcc ggccgatgac ctatctcggc ctcgattgcg
     3301 gcacctcggc cttgaaggcg gctttggtcg atgaggacga gcgcgtcctg gcgacggctg
     3361 tcgaagccta tcagccggtt catccgcggc ccttatggtc ggagcagaat cctgacgact
     3421 ggctcgccgc aatgttcgcc gcgctcgcgc ggctggcgcg cgaccggccg gaggcgatgg
     3481 cgcgcgtcgc ggcgatcggc ttttccggcc agatgcacag cgccgtgctg ctcgacggcg
     3541 ccgacaagcc gttgcggccg gcgatcctgc acaacgacac gcgcgctttc gccgaagcgg
     3601 cgcgcctcgc cacgcgccat gccgagctga tcgcgcgcgt cggcgtcaag ccgatggccg
     3661 gattcaccgc gccgaaattg atgtggctc
//
LOCUS       DATGJW010000151         7654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_857704, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000151 DATGJW010000000
VERSION     DATGJW010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7654
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_857704"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            408..740
                     /locus_tag="VKU03_04550"
     CDS             408..740
                     /locus_tag="VKU03_04550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173355.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70562.1"
                     /translation="MRASMTKAVAVSLTALALGVATIGASTPATAAMWHGGGHWHGGG
                     WHGGWHGGWRHGGWGWAPFAFGALAGAALAAPYYYGGYYGYNACVTYQPMYDAWGNYI
                     GQQAVNVC"
     gene            920..1183
                     /locus_tag="VKU03_04555"
     CDS             920..1183
                     /locus_tag="VKU03_04555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70563.1"
                     /translation="MSAIAKKTIASLAALALGAAVLGASPASAHSWHGGWHHHGYWGP
                     GVAFGVLGLAAGAIAVSQYECVRYRPVYDSYGNYVGRQAVNIC"
     gene            1415..2077
                     /locus_tag="VKU03_04560"
     CDS             1415..2077
                     /locus_tag="VKU03_04560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70564.1"
                     /translation="MRNSVKTAVLAAASALALAFAAAPASAALYHGGGGFHSGFHGGG
                     TFRGGGSFHGAGTFRGGMSAFHANAVRNTAFGGGHMWGHRGWAGHPWAGGWNGGWGGG
                     AYRNVALANHGWTGRRWAGGWDHHWRGGWHRGWRHRYWAGGWGWGAPYWGASWGWSYP
                     YVAEYDYGYPSYVAEYDYGYPYYGAACAPYWNGYAYVYPNTLACAGGVVINTPLISFG
                     VY"
     gene            complement(2071..3201)
                     /locus_tag="VKU03_04565"
     CDS             complement(2071..3201)
                     /locus_tag="VKU03_04565"
                     /EC_number="2.7.1.170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009254 - peptidoglycan turnover [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anhydro-N-acetylmuramic acid kinase"
                     /protein_id="HLJ70565.1"
                     /translation="MSGAPPRLVAAIGVMSGTSMDGIDVAILASDGESLVRPGPGATF
                     SYPEETRAALRKIIADPQAARQPLVELEAAVTEAHVAAVESFMKAYKLDPQSVALVGL
                     HGQTVLHRPRERFTRQLMDGAQAAKRLGLDVVCDFRAADIAAGGEGAPLAPLYHAAVA
                     ATAPRPLAVLNWGGVGNVTYLGAAGEIVAFDAGPANALIDDFLQRRRGVSHDADGALA
                     ASGRVNKRILARLMSDPYFDRRPPKSLDRNHFHALAGAVDGLSDADGAATLTAFTVDA
                     TAAATRFFERAPLRWLVTGGGRRNRTLMESLRRRLGAPVDPIEAAGHDGDALEAHCFA
                     YLAIRSRRGLPLSLPTTTGAPRALTGGAFWPAPQAARLARAQ"
     gene            complement(3198..3896)
                     /locus_tag="VKU03_04570"
     CDS             complement(3198..3896)
                     /locus_tag="VKU03_04570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406257.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLJ70566.1"
                     /translation="MSKYLFEPPPPPAVPIAGRDQLYPVARIFCVGRNYEEHAREMGH
                     VVDREKPFYFSKSATTLRQDGATLPYPPGTTNYHFECELVVAIGAAAFKIAAHQAEAA
                     IFGYACGLDMTRRDLQLSERAKQRPWTLGKDVEASAPVSAIAPASAIGHPKRAHIELR
                     QNGEIRQASDIAELVWSVPELIAHLSGFYHLAPGDLVFTGTPAGVGPVQPGDRLEASI
                     EGVATLAVTIGAAP"
     gene            complement(4089..4700)
                     /locus_tag="VKU03_04575"
     CDS             complement(4089..4700)
                     /locus_tag="VKU03_04575"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLJ70567.1"
                     /translation="MVKLNKIYTRTGDSGSTALGDGQRRRKDDARVAAFGAVDETNSA
                     IGLARIATAGSADPDLAAIDRVLARAQNDLFDLGADLCMPGEKAVASAKAKRPALRVT
                     MDQVAALEKAIDALNAGLSPLRSFVLPGGGEAAATLHLARTICRRAERDIVALSHIEG
                     EEVGAAALAYINRLSDYLFVAARTANRRGEGDVLWVPGANQGG"
     gene            complement(4723..4914)
                     /locus_tag="VKU03_04580"
     CDS             complement(4723..4914)
                     /locus_tag="VKU03_04580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin transmembrane helix small protein"
                     /protein_id="HLJ70568.1"
                     /translation="MHAFSNIGVGVALAAVALVLLLGLANMLRGGAANTSQALMRWRV
                     GLQFAAIVLIVLVLLTRMH"
     gene            complement(4986..7580)
                     /locus_tag="VKU03_04585"
     CDS             complement(4986..7580)
                     /locus_tag="VKU03_04585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02302 family protein"
                     /protein_id="HLJ70569.1"
                     /translation="MSDGARTPGSEPQPRIPDAAATRERLRRLARQASWILTWERVWP
                     PVAWALTVLLLFLACSWFGLWFSAPPWARGVGVALFAAALIAALAPLAWTLVSRKRDA
                     DALARLDRDSGAPHRPASSSMDRLADSAADEATRALWRLHQRRLAENVAKIALAPPSP
                     RMVEKDRLALRLGVLVLAVAAAFVAGPEKYARVAAAFDWRLDMRPAAGFRLDAWIDPP
                     AFTGRPPIMLDVLGKTEKADGPTRIETPAGSILVVRADPQTASAKVEGALTPVEPGKA
                     DGANRTSRDPRERRWTIKGAGKLTIERGGSTLAAFNIIGLPNGKPTIALTAPPHANAH
                     GSLTLRYRIADQYGVAAAEADFVKPPPSDGNPAPRSLVEPPHMGLQLPPSQGGTGEAQ
                     TTGDLSEHLWAGAKVLMTLRADNVAGEEGVSEATEVRLPQRFFSNPLAKALVEQRRDL
                     ALDPDHNRGRVGETLDALTIAPDFFKISASVYLGLRAAQTRLDNARSDGDLLGVCDLL
                     WAMALQIEDGDATQAERDLRAAEQRLREALDRGASDEEIRELMKELRQAAENFARELA
                     ENAQKQAGADQQGQDQDDQMLNEQDLQSLLDRMEETARSGDKAEAQATLDQLQNLFEN
                     LRGARRGGASQAERQMRKALGDVDRLLRDQQKLRDDTFRQDQRARRRSGSPDAQQQQG
                     QPGDESADSGQEQPGDGTPLDERQKALRDRLNDLQRRMKGLGLKSEQGFDDADRAMGD
                     AEGDLKQGQNGRGKAIGDQGRALEALRRGAQGMQQQMQGEQGSGEGEAEIRPGRGQGQ
                     RGRDPLGREFGPQGRGAAMGPLGDTIGAAERARRVMEELRRRLSDPNRPGDERDYLER
                     LLNRNLPD"
BASE COUNT         1127 a         2539 c         2697 g         1291 t
ORIGIN      
        1 agccgcttga gctatggcgg ggccgcgccc gccaatgtcc gccgccaggc gcggctctgg
       61 ctgaagcgcc tgtccaagag cttggcggcg gcgagcgtca cgcagagttg aaacgcgcga
      121 caagtcgtcg cgctgggcaa acctaaacga atattcatgg cttgcgccgc gcctgcccgg
      181 cttttctgaa cgaatattaa tgtcgctcag ataaatgctt tgctatctaa aaggggctct
      241 tgaagcggcg ctgaagcgtc ttatgtcaac cctcaagaat cctctgccga gtgcgagaac
      301 cttttccgtt tgaaggcgtt tggagcgatg agaccaatgc gcttcggcaa agcggggcct
      361 ggcggcggcc ttaacgtttt cgccgaaaga gagagatgga ggtagacatg cgcgcttcga
      421 tgacgaaagc cgtcgcggtc tcgctgacgg cgctcgctct gggtgtggcg acaatcggcg
      481 cctccacgcc ggctaccgcg gccatgtggc atggcggcgg acactggcat ggcggcggct
      541 ggcacggtgg gtggcatggc ggctggcgtc atggcggctg gggctgggcg cccttcgcct
      601 tcggcgccct ggccggcgca gcgctcgccg cgccttacta ttacggcggc tactatggct
      661 ataatgcctg cgtgacttac cagccgatgt atgacgcctg gggcaactac atcggtcagc
      721 aggcggtgaa tgtgtgctga taaagcacga cgagttcgcc ggctacccca agccggcgct
      781 cggaacgctg cgcccctcag cgttccccgt tcctggcccc gcttcggcgg ggcctttttt
      841 tgtccggcga gaagcatccc gtaactgaaa tccgtgatcg cggcggcgcc gcgagccctt
      901 cgatacggga gttgaatcta tgtccgccat cgccaaaaag accatcgctt cgcttgctgc
      961 cctcgccctg ggggcggccg ttctcggcgc gagccccgcc tccgctcatt cttggcacgg
     1021 cggctggcat catcatggct attggggccc gggcgtcgct ttcggcgtgc tcggcctcgc
     1081 ggccggcgcg atcgccgttt cgcaatacga atgcgtccgc tatcggccgg tttatgacag
     1141 ctacggcaac tatgtcggcc ggcaggcggt caatatctgc tgacgatgcg ctgctcaagc
     1201 cggcccgctc ggccggcttt gtagggacgc tgcgcccctc agcgttcccg ttgccctggc
     1261 cccgcttcgg cggggccttt tttgcgctcc gccgaggtac gataaaatcg aattcattgc
     1321 cgcgcgccgc tggaactggt ccgaagcagg cccgttcctg acctatcaga gcgaacgggc
     1381 ctttcgctcg cttttaggtt gcggagaatg aaatatgcgt aattcggtca agacagccgt
     1441 cctcgcggcg gcgtcggcgc tggcgctggc ttttgccgcc gcgcccgcgt cggcggccct
     1501 ctatcacggc ggcggcggat tccacagcgg ctttcacggc ggcgggacgt ttcgtggcgg
     1561 cggcagtttt cacggcgccg gaacgttccg gggcggcatg agcgccttcc atgccaacgc
     1621 cgttcgtaac accgcgtttg gcggcggcca tatgtggggt catcgaggct gggccggtca
     1681 tccctgggcc ggcgggtgga atggcggctg gggcggcggc gcctaccgca acgttgcgct
     1741 cgccaatcat ggctggactg gacggcgttg ggccggcggg tgggatcacc attggcgtgg
     1801 cggctggcat cgcggctggc gccatcgtta ctgggcgggc ggctggggct ggggcgctcc
     1861 ctactggggc gcaagctggg gctggagcta cccctacgtc gcagaatatg actacggcta
     1921 tccctcctat gtcgccgaat acgattacgg atatccgtat tacggcgccg cctgcgcgcc
     1981 ctattggaac ggctacgctt acgtctatcc caatactttg gcctgcgccg gcggcgtggt
     2041 catcaacacg ccgctgatca gcttcggcgt ctactgagcg cgcgcgagcc gggccgcttg
     2101 cggcgcgggc cagaacgcgc cgccggtcag cgcccgcggc gcgccggttg tcgtcggcag
     2161 agacaagggc aagccgcgcc gcgagcggat ggcgagatag gcgaagcaat gcgcttcgag
     2221 cgcgtcgccg tcgtgaccgg cggcttcgat cggatcgacc ggcgcgccga gccgccgccg
     2281 cagcgactcc ataagcgtgc ggttgcgccg gccgccgccg gtgaccagcc agcggagcgg
     2341 cgcgcgctcg aaaaagcgcg tcgcggccgc ggtcgcgtcg accgtgaagg ccgtgagggt
     2401 ggcggcgccg tcggcgtcgg aaagcccgtc cacagccccc gccagggcat ggaaatgatt
     2461 gcggtcgagc gatttcggcg gccgtcgatc gaaataggga tcgctcatca ggcgcgcgag
     2521 aattcgtttg ttgacgcggc cgctggcggc gagcgcgccg tcggcgtcat ggctgacgcc
     2581 gcgccggcgc tgcaggaaat cgtcgatcaa agcattggcc gggccggcgt cgaaggcgac
     2641 gatctcgccg gccgcgccga gataggtcac gttgccgacc ccgccccaat tgagaaccgc
     2701 caggggccga ggcgccgtcg ctgcaaccgc cgcgtgatag agcggcgcga gcggcgcgcc
     2761 ttcgccgccg gcggcgatgt cggcggcgcg aaaatcgcag accacgtcga ggccgagccg
     2821 tttggccgcc tgcgctccat ccataagctg ccgggtgaaa cgctcgcgcg gccggtgcag
     2881 aacggtctgg ccatgcaggc cgaccagcgc gacgctttgc ggatcgagct tatacgcctt
     2941 catgaagctt tcgaccgcag cgacatgcgc ctcagtcacc gccgcttcga gctcgacgag
     3001 cggctgccgg gcggcctgcg gatcggcgat gatcttgcgc agagcggcgc gagtctcctc
     3061 cggataggaa aaggtcgcgc cggggcccgg ccgaaccagg ctctcgccgt cgctggcgag
     3121 gatcgcgacg tcgattccat ccatcgacgt accgctcatc acgccgatcg cggcgacgag
     3181 gcgaggcggc gcgccgctca cggcgccgcg ccgatcgtga cggcgagcgt ggcgacgccc
     3241 tcgatcgagg cttccagccg gtcgcccggc tggaccggtc caaccccggc cggcgtgccg
     3301 gtgaagacga gatcgcccgg cgccaagtga tagaagccgg aaagatgcgc gatgagttcg
     3361 gggaccgacc agacgagctc ggcgatgtcg ctcgcctgcc ggatctcgcc gttctggcgc
     3421 agctcgatat gggcgcgttt gggatggccg atcgccgaag cgggcgcgat cgccgagacc
     3481 ggcgcgcttg cctcgacatc cttgcccagc gtccacggcc tttgcttggc gcgctcggag
     3541 agctggaggt cgcggcgcgt catgtcgagg ccgcaggcat agccgaaaat cgccgcctcg
     3601 gcctggtgcg ccgcgatctt gaatgctgcg gcgccgatcg cgacgaccag ctcgcattcg
     3661 aaatgatagt tcgtcgtgcc aggcggatag gggagcgtcg cgccgtcctg gcgcagcgtg
     3721 gtcgcgctct tggaaaagta gaagggcttc tcgcggtcga cgacatggcc catttcgcgg
     3781 gcgtgctctt catagttgcg gccgacgcag aagatgcggg cgacgggata gagctgatcg
     3841 cgcccggcga tcggcacggc ggggggcggc ggcggctcga aaaggtactt gctcatatcc
     3901 gttggctcat gatgctggtt tcgcgccgct cgccctcatg cggccatagg ttttgtagcc
     3961 gcggtcggcg accgcggcta cagcgcgacg gccgatgaag cgcaaggcgc gccccttctc
     4021 cggcttgcga gagagggttg gggtgagggt aagctgcaag tccatccatc cgcgaataag
     4081 gtacgttctc acccgccttg attggccccg ggaacccaga ggacgtcgcc ttcgccgcgc
     4141 cgattggcgg tgcgcgccgc gacgaagaga taatcggaaa gacggttgat ataggcgagc
     4201 gccgcggcgc cgacctcctc gccctcgata tggctcaatg cgacgatgtc gcgctcggct
     4261 cgccggcaga tcgtgcgggc gaggtgaagc gtcgcggcgg cctcgccgcc ccccggcagg
     4321 acgaaggagc gcaaaggcga aagcccggcg ttgagcgcgt cgatcgcttt ctccagcgcc
     4381 gcgacctggt ccatcgtgac gcgcagcgcc gggcgcttgg ccttggcgct ggcgacggcc
     4441 ttttcgccgg gcatgcagag atcggcgccg agatcgaaga gatcgttctg ggcccgcgcc
     4501 agaacgcggt cgatcgccgc cagatcggga tcggccgagc cggccgtcgc aatccgggcg
     4561 aggccgatcg cgctgttggt ctcgtcgacc gcgccaaagg cggcgacgcg ggcgtcgtcc
     4621 ttgcggcggc gctggccgtc gccgagcgcg gtcgagccgg aatcgccggt gcgggtgtaa
     4681 atcttgttga gcttcaccat cgtcgttcgg ccttgagccg aatcaatgca tgcgcgtcaa
     4741 aagcacaagg acgatgagga caatggcggc gaattgcaag ccaacgcgcc agcgcatcag
     4801 cgcttgcgac gtgttggcgg cgccgccgcg cagcatgttg gcgaggccga gcaggagaac
     4861 gagcgcgacc gcggccagcg cgacgccgac gccgatattc gaaaaggcat gcatcgcgcg
     4921 cctcgctttt attcgttcaa aagcgatcta tagcaggttt gcgaaaacga gaagcggagc
     4981 cggcctcaat ccggcaggtt gcggttgagc aggcgctcga gatagtcgcg ctcgtcgccc
     5041 ggccgattgg ggtcggagag gcggcggcgc aattcctcca tcacccggcg ggcgcgctcg
     5101 gcggcgccga tcgtatcgcc cagcggaccc atcgcagcgc cgcggccttg cgggccgaat
     5161 tcgcggccga gcggatcgcg ccctctttgg ccctgtcccc tgccgggccg aatctcggcc
     5221 tcgccttcgc cggagccctg ctcgccctgc atctgctgct gcatgccctg ggcgccgcgg
     5281 cgcagggcct ccagggcgcg gccttgatcc ccgatcgctt tgccgcggcc gttctgccct
     5341 tgcttgagat cgccttcggc gtcgcccatg gcgcggtcgg cgtcgtcgaa accttgctcg
     5401 cttttcaggc cgagcccctt catgcggcgc tgcaaatcgt tgagccggtc gcgcaaagcc
     5461 ttctgccgct cgtcgagcgg cgtcccgtcg cccggctgct cctggccgga atcggcgctt
     5521 tcgtcgccgg gctggccctg ctgctgctgc gcgtcgggcg agcccgagcg gcggcgcgcc
     5581 cgttgatcct ggcggaacgt gtcgtcgcgc aatttctgct ggtcgcggag cagcctgtcg
     5641 acatcgccga gcgccttccg catctggcgc tcggcctggc tcgcgccgcc gcgccgcgcg
     5701 ccgcgcaaat tctcgaacag gttctgcaat tgatcgagcg tcgcctgcgc ctcggcctta
     5761 tcgccgctgc gcgccgtctc ctccatccgg tcgagcagac tctgtagatc ctgctcgttg
     5821 agcatttggt cgtcttgatc ctggccctgc tggtccgcgc cggcctgctt ttgcgcgttc
     5881 tcggcgagct cgcgggcgaa attctcggcc gcttggcgca gctccttcat caattcgcga
     5941 atctcttcgt cgctcgcgcc gcggtcgagc gcttcgcgca ggcgctgctc ggcggcgcgc
     6001 agatcgcgct cggcttgcgt ggcgtcgccg tcctcgatct gcagcgccat cgcccacagg
     6061 agatcgcaga cgccgagaag gtcgccgtcg gaccgcgcgt tgtcgaggcg ggtttgcgcg
     6121 gcgcgcaggc cgagatagac gctggcgctg atcttgaaga aatccggcgc gatcgtcagc
     6181 gcgtcgagcg tctcgcccac gcggccgcga ttgtgatccg gatcgagcgc caagtcgcgg
     6241 cgctgctcga ccaaagcctt ggcgagcggg ttggagaaga agcgctgcgg cagcctgacc
     6301 tcggtcgcct cgctcacccc ttcctccccg gcgacattgt cggcgcgcag ggtcatcagg
     6361 actttcgcgc ccgcccagag atgctcggaa aggtcgccgg tcgtttgcgc ttcgccggtt
     6421 ccgccctggc tcggcggcaa ttgcaggccc atatgcggcg gctcgaccag cgagcgcggc
     6481 gccggattgc cgtcgctcgg cggcggcttg acgaaatcgg cctccgcggc ggcgacgcca
     6541 tattgatctg cgatgcggta gcgaagcgtc agcgagccgt gcgcattggc gtgcggcggc
     6601 gcggtcaagg cgatggtcgg cttgccgttg ggcaggccga tgatgttaaa ggcggcgagg
     6661 gtcgagccgc cgcgttcgat cgtcagcttg ccggcgcctt tgatcgtcca gcgccgctcc
     6721 cgcggatcgc ggctcgtgcg gttggccccg tccgctttgc cgggttcgac cggcgtcaag
     6781 gcgccttcga ccttggccga cgcggtttgc gggtccgccc tgacgacgag gatcgagccg
     6841 gccggcgttt cgatcctggt cgggccgtcc gccttctccg tcttgccgag aacgtcgagc
     6901 atgatcggcg gccggccggt aaaggccggc ggatcgatcc aggcgtcgag ccggaagccc
     6961 gccgccggcc gcatatccaa acgccagtcg aaggcggccg cgacgcgggc gtatttttcc
     7021 ggcccggcga cgaaagccgc ggcgaccgcc aagaccagga cgccgaggcg caaggcgagg
     7081 cggtccttct cgaccatgcg cggcgagggc ggagcgagcg cgattttcgc gacgttttcg
     7141 gcgagccggc gctgatgcag gcgccaaagc gcgcgggtcg cctcgtcggc ggccgaatcg
     7201 gcgagccgat ccatgctgga ggaagccggg cgatgcggcg cgccgctgtc gcgatcaagc
     7261 cgggccaagg cgtcggcgtc gcgctttcgc gagacgagcg tccaagcgag cggcgcgagg
     7321 gcggcgatga gggcggcggc gaagagcgcg accccgacgc cgcgcgccca gggcggcgcg
     7381 gagaaccaca ggccgaacca ggagcaggcg aggaacagca ggaggacggt cagcgcccag
     7441 gcgacaggcg gccagacgcg ctcccaggtc agaatccagg acgcctggcg ggcgaggcgg
     7501 cgcagccgct cccgcgtcgc cgcagcgtcc gggatccggg gctgcggctc gctgccgggc
     7561 gttcgcgcgc catcgctcaa tggcttctcc ttcgccgggg cggcgcgttc agcctgacgc
     7621 caaccaaggc ggaattttgt cgaggccgat caat
//
LOCUS       DATGJW010000152        10515 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1272445, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000152 DATGJW010000000
VERSION     DATGJW010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10515)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10515)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10515
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1272445"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(211..1191)
                     /locus_tag="VKU03_04590"
     CDS             complement(211..1191)
                     /locus_tag="VKU03_04590"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLJ70570.1"
                     /translation="MPISGACWAEASETGANMDLRKLRYFAGVVEAKSISKAAEALHV
                     AQPALSKSVRALELDLETPLLERSAQGVTTTEAGARLYEHCQLLFKQIARARLDVRKS
                     VEKPSGLVTIGMPHSLMSVLALPLLDAMTKFYPDVRLELKQEQSHLLANAVRSNKLDC
                     AAMASQQSHAGLVCRPLLVEELFFIEPRRAAQGGSEAQISFAEASRRQFVLPAVGNGL
                     RAYIEGHFRARSLPLDVRYEIDAIALMTQCVDAGLGVSLLPGGCLQRDAYYPRMSAKS
                     FAEGGCHRHIVLSHSADGVLNPAAARALALLEDVARGLVARGQWLGGRAA"
     gene            1349..2209
                     /locus_tag="VKU03_04595"
     CDS             1349..2209
                     /locus_tag="VKU03_04595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HLJ70571.1"
                     /translation="MRSKLFVPGSRPEFFEKAWRSEADALSFDLEDAVVEAGKAEARA
                     RVAEAVAAAPAQRTKMLIVRANAVETPHFEADLEAVVGPGLDIVNLPKVESPQAVVQA
                     CRLIERLEQARGLKRRIGVLVNIETPRGLRRAAEIAAATDRIVGLQVGFGDLFAPLGV
                     VRTLATLTPVWLAVRLAAAEAGLPAYDGAFVAVSDLAGFRAEAEAARAAGFAGKSCVH
                     PSQIATANAIFFPSAAEIERARRIVAAADEKQARGVGAFTVEGVLADGPFITRARDVL
                     RLAQRTNANG"
     gene            2202..3458
                     /locus_tag="VKU03_04600"
     CDS             2202..3458
                     /locus_tag="VKU03_04600"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase"
                     /protein_id="HLJ70572.1"
                     /translation="MADRPGSPAPAADGALKGLRVIDCATLFAGPLIGTLMGDFGADV
                     IKIEHPSGDPLRNMGAKKNGHGLWWKTSARNKRCIALDLKTADGAEIFKALIADADIL
                     IENFRTGTLESWGLGWDVLSALNPRLIMVRVTGFGQTGPYRRRAGFGTLAEAISGFAH
                     ITGQPDGPPTLPPFGLADGIAAYFGCFAAMFALYERDRRDGGRGQFVDLAIYEPIFAL
                     LGPQPIIYDQLGVIQNRTGNRSVNNAPRNAYRTRDGRWVALSAAAPSIVRRVLELTGG
                     EAAANDPRFQTNADRIRHVEEIDAIVGGWIARHDLKEVLSAFEKVEAAIAPIYDIAQI
                     FEDEQYAARKSIATVDDEDLGPVRMQNVFPLLSRTPGAIRFAGPRIDQHRDEILRELR
                     QAGRLAPSSAEADGALPGAAEEKRKS"
     gene            3455..4372
                     /locus_tag="VKU03_04605"
     CDS             3455..4372
                     /locus_tag="VKU03_04605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HLJ70573.1"
                     /translation="MTAPRSNPRDLVGYADRRPHPRWPGEARIAVQFAINYEEGSEYT
                     AIDGDGRTELGLAEAPGGRVPAGQRDLAFETMYEYGSRVGIWRLFRLFGERRLPVTIF
                     GCALALERNPRVAEAIAGAGYDVCCHGWRWEEHFRLGESEERERIARAVASIAKSVGE
                     RPLGWYCRYGPSENTRRLLVEEGGFLYDSDAYNDELPYWVEVSGKPHLVVPYTMDAND
                     GKFAQPAGFGTSADFETYLKATFDQLYEEGAEKAGLMSIGLHPRLAGRPARARALAGF
                     LDYILKRDRVWICRRVDIARHWRSEHPAH"
     gene            4386..5090
                     /locus_tag="VKU03_04610"
     CDS             4386..5090
                     /locus_tag="VKU03_04610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014491737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HLJ70574.1"
                     /translation="MMSDAPWEAYLTEADRSVLARGRFGRRMGFGEKPAVIVIDCQRY
                     MVGERGVEDGRYPSSCGALGWAAVDRIAAILDAARLVKTPVFLTRFALDPSGADIGVY
                     ARKRDFLQRSDWCLEGAEGAELLPEVGPKPGDIVFVKKKPSAFHGTPLLGYLIDRRID
                     TVIVLGGATSNCVRATVFDAASYNFRAIVPADAVFDRLPISHAINLFDMDRQFADVVN
                     SDDAIAALGRTARAAS"
     gene            5211..6296
                     /locus_tag="VKU03_04615"
     CDS             5211..6296
                     /locus_tag="VKU03_04615"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70575.1"
                     /translation="MKSAPSEIRAETDLADRYGRRNGGAAADPSSAGFESVTRADQQR
                     RRRRNLRLLIYRYAVVLALAVFVVGFSLLLPRTFFTLGNFRTIVSSQAVLMILALGLT
                     LPLTTGEFDLSIGSMLGCASVLTAYFTGELHWPLALAILLTVLTGVAIGVLNGIFVVR
                     VGVNAFIGTLGASTILTGVTLAVSGGQILNNVAQPLVDFVQFQVFGLAAPVYIGFLLA
                     LLLWYLYEHTPTGRHLYFVGEGREAARLVGLPVDRLRFSAFVASGVISAAAGIFTAGQ
                     LGAADPSVGPSFLLPAYAAAFLGATTIKPGRFNAWGTVVALYLLVTGVTGLELMGTSS
                     WVQEMFNGAALLIAVTFARFVARDQAA"
     gene            6386..7453
                     /locus_tag="VKU03_04620"
     CDS             6386..7453
                     /locus_tag="VKU03_04620"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ70576.1"
                     /translation="MAALIAGFSAYASSAQADGLQDAKAIVGKAMAKPQFVAPGEPFD
                     IGKLKGKRIAIITSTLAVPFVATIAHGVEAAAKAAGMETSLFDGKGDVSEWNRGMAQA
                     VSQHADGIVTVGASPELMKGPTADALKAHIPVVDAVTADKTAPLVPGTFSHVSISFYH
                     SGQLQADEAIAKSGGKAHVLILGDNEFPGEVSRVEGMQKEFSTLCPDCTVTVQDTQVG
                     NLGVKLGQQTQTLLRRSPDINIVLVTYDAQAIYVVPAIKAANFSNPIEVIGADAVPSN
                     LDWIRQGNIQIADVGEPEVWLGWAAVDEVARGMLALPKVDEQIPLRLFVKENLKDVSN
                     DENELFGGDFVAEYKKLWGLK"
     gene            7481..8989
                     /locus_tag="VKU03_04625"
     CDS             7481..8989
                     /locus_tag="VKU03_04625"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ70577.1"
                     /translation="MTSGDAALLELHGLDKSFAGVRVLKSVDLSVRRGETHALIGQNG
                     SGKSTLIKILSGYHAPDAGRIVMRGAPVPLPLRPGEAAAAGLRFLHQDIGVVRTMTVL
                     ENLRIGRFRTSPYGRLLWRSEREAAKAILAQLQLDIDPDMPVRDLPGAERALIGFARA
                     SQEMDHARGEALILDEPTAFLPGPSVEKLFAAIREITRRGSAVVFVSHRLDEVMAIAD
                     RISILRDGALVETVAKADTNERKLVTAMLGREMDALYPTRPAASGERMLGVEGLTGRI
                     ASNVAFTVQKGEIVGLTGLLGMGHDEVPYLLFGAQPAVQGSIAIEGRALAEFTPRKAF
                     EAGMAFLPADRQRQSGVPKATVLENVSMTDGRRFVKHGRLRRDLERSAVETVLELFDV
                     RPPDPDRPLATLSGGNQQKALLGKWLRTKPKVILLHEPTQGVDIGSRKQVLQIIAQAA
                     AAGAAIVVASAEYEELAHTCHRVLIFRRGRIVRELAGNDLTAARIAEQCYLS"
     gene            complement(9016..10071)
                     /locus_tag="VKU03_04630"
     CDS             complement(9016..10071)
                     /locus_tag="VKU03_04630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018643399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLJ70578.1"
                     /translation="MTAEQEPALARHQALFGADELWLKQYSEEIIEPDLPIVDPHHHL
                     WDRGARYLFDELRADIETGHRIEATVFVQCDAMYRASGDPAFAPLGETEFVNGVAAMS
                     ASGLYGPARVCAGIVGYADLQLGAKVDAVLEAHLRAAGDRFRGVRGRSVWDADPTIKG
                     SSRDFPKGLLLDAAFREGYARLAAYGLSFDSWLFHPQIPELADLAGRFPETTVILDHI
                     GAPLAIGAYAGKRQEVFAHWRRNMTELARRPNVTVKLGGLAMHLFGFDLDAAHRPTPA
                     SSAELADLWRPYIETCIELFGVERCMFESNFPVDKRGVSYPVLWNAFKRLSAAASAAE
                     KAALFKGTACRAYRLNL"
     gene            complement(10233..>10515)
                     /locus_tag="VKU03_04635"
     CDS             complement(10233..>10515)
                     /locus_tag="VKU03_04635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF488 domain-containing protein"
                     /protein_id="HLJ70579.1"
                     /translation="FHNYADYALSERFRVGLEHLVEVGRKRRCVIMCSEAVWWRCHRR
                     IIADHLIARGETVFHIMGAGRLEPAHLTEGAVIQPDGMVVYPRANQTSA"
BASE COUNT         1846 a         3427 c         3389 g         1853 t
ORIGIN      
        1 gatataattt ggagcggtag ccgcggcttt ccgcagggag cgagaagagg caaacagaaa
       61 atatgtttat ccattttttg cgcgcaaacc ctttgaaaaa cctcaatttg gccaaataaa
      121 gatttggaaa ttcttggaga aatttggaaa aagcttggaa aaatttggcg ccgggcttcc
      181 gagccctcga cgaccgcgtg cgctttcgcc tcaagccgcc cgcccgccga gccattggcc
      241 gcgcgcgacc agtccgcgcg ctacatcttc gagcagcgcg agcgcccgcg ccgcggcggg
      301 gttgagaacg ccgtcggcgg aatgggaaag gacgatgtgg cgatggcagc cgccttcggc
      361 gaaggacttc gcgctcatcc gcggatagta ggcgtcgcgc tgcaagcagc cgccgggcaa
      421 caacgaaacg ccgaggccgg cgtcgacgca ttgcgtcatc agcgcgatgg cgtcgatttc
      481 gtacctcaca tcgagaggca gcgaccgcgc ccggaaatgg ccctcgatat aagccctgag
      541 cccattgccg acggcgggca ggacgaattg ccgccgcgac gcctcggcga aggaaatctg
      601 cgcttcggag ccgccctgag ccgcccgacg cggctcgatg aagaacaatt cttcgacaag
      661 cagcggccgg cagaccaagc cggcatgcga ttgctggctg gccatcgccg cgcaatcgag
      721 cttgttcgag cggaccgcgt tggcgaggag atggctctgc tcctgcttca gctccaaacg
      781 tacgtccgga tagaatttgg tcatcgcatc gagcagcggc aaggcgagga cgctcatcaa
      841 gctatgcggc atgccgatcg tgacgaggcc ggacggcttc tcgaccgatt tgcgcacgtc
      901 gagccgcgcg cgggcgatct gcttgaacag aagctgacaa tgctcgtaga gccgcgcgcc
      961 ggcctcggtc gtcgtcacgc cttgcgccga gcgctcgagg agcggcgtct ctaaatcgag
     1021 ttcaagcgcc cggacgctct tgctcaaggc cggctgcgcg acatgcagcg cctcggccgc
     1081 tttgctgatg ctcttcgcct cgaccacgcc ggcgaagtag cgcaattttc tgaggtccat
     1141 attggcgccg gtctcgctcg cttcggccca gcatgcgccg gaaatgggca tatcgtccag
     1201 gaatatccct ccagccattt actattggcg gttttcgccg atggcgcggc aaaatggccg
     1261 ccaaggcgag cgaagttcgt cgatttcggg ctctcgcgcc gccccagatc ataacagacc
     1321 gttatggccg ggccggaacg gagcgatcat gcgaagcaag cttttcgtgc cgggatcgcg
     1381 gccggaattt ttcgagaagg cctggcggtc cgaggccgac gcgctctcgt tcgacctcga
     1441 agacgcggtc gtcgaggcgg gcaaggccga ggcccgcgcc cgggtcgcgg aggcggtcgc
     1501 cgcggcgccg gcccaacgga cgaaaatgct catcgtcagg gcgaacgcgg tcgaaacccc
     1561 gcatttcgag gccgatctcg aagccgttgt cgggccgggc ctcgacatcg tcaatctgcc
     1621 gaaggtcgag agtccgcagg ccgtcgtcca agcgtgccgc ctgatcgaac ggctcgaaca
     1681 ggcgcgcggc ctgaagcggc gcatcggcgt cctggtcaat atcgaaacgc cgcgcggcct
     1741 gcgccgggcc gccgagatcg ccgccgctac ggaccggatc gtcgggctgc aagtcggctt
     1801 cggcgatctg ttcgcgccgc tcggcgtcgt gcggacgctc gcgaccctca cgcctgtctg
     1861 gctggcggtc cgcctcgccg ccgcggaggc cggactgccg gcctatgacg gcgccttcgt
     1921 cgccgtctcc gacctcgccg gctttcgcgc cgaggcggag gccgcccggg ccgccggctt
     1981 cgccggcaag agctgcgtcc atccgagcca gatcgcgacg gccaatgcga tcttctttcc
     2041 aagcgccgcc gagatcgagc gcgcccggcg catcgtcgcc gccgcggacg agaagcaggc
     2101 gcgcggcgtc ggcgctttta cggtggaggg cgtcctcgcc gacggcccgt tcattacgcg
     2161 cgcccgcgac gtgctgcggc tcgcgcaaag gacgaacgcc aatggctgat cggcccggct
     2221 ccccggctcc ggccgctgac ggcgcgctga aagggctgcg cgtcatcgat tgcgcgacgc
     2281 tcttcgccgg gccgctgatc ggcacgctga tgggcgattt cggcgccgac gtgatcaaga
     2341 tcgagcatcc aagcggcgat ccgctgcgca atatgggcgc gaagaagaac ggccacgggc
     2401 tgtggtggaa gacaagcgcc cgcaacaagc gctgcatcgc gctcgatctc aagacggcgg
     2461 acggcgccga aatcttcaag gcgctgatcg ccgacgccga catcctcatc gagaattttc
     2521 gtaccggaac gcttgaatcc tggggtctcg gctgggatgt gctttccgcg ctcaatccgc
     2581 gcctcatcat ggttcgggtg accggcttcg gccagaccgg cccctatcgc cggcgcgccg
     2641 gtttcggcac tttggccgag gcgatatcgg gcttcgctca cattaccggc cagccggacg
     2701 ggccgccgac tttgccgccc ttcgggctcg ccgatggaat cgcggcctat ttcggctgct
     2761 tcgccgcgat gttcgcgctc tacgagcgcg atcggcgcga cggcgggcgc ggccagttcg
     2821 tcgaccttgc gatctacgag ccgatcttcg cgctgctcgg cccgcagccg atcatctatg
     2881 atcagcttgg cgtgatccag aacagaaccg gcaatcgatc ggtcaacaac gcgccgcgca
     2941 acgcctatcg cacgcgcgac ggccgctggg tcgccttatc cgccgccgcg ccgagcatcg
     3001 tccggcgcgt cctcgaattg accggcggcg aagcggccgc gaacgatcct cgtttccaga
     3061 cgaacgccga ccgcatccgg catgtcgagg agatcgacgc cattgtcggc ggctggatcg
     3121 cccggcatga tcttaaagaa gttctctcgg ccttcgagaa ggtcgaggcg gcgatcgcgc
     3181 cgatttacga catcgcgcag atttttgaag acgagcaata cgcggcccgc aaatcgatcg
     3241 cgacggtcga cgacgaagat cttggtccgg tgcggatgca gaacgtcttt ccgcttctgt
     3301 cgcggacgcc cggcgccatt cgcttcgccg gcccgcgcat cgatcagcac cgcgacgaaa
     3361 tcctccgcga attgcggcag gcggggcgat tggcgccctc ctccgccgag gcggacggcg
     3421 ccctgccggg cgcggccgaa gagaagcgca aatcatgacc gcgccccgct caaatccgcg
     3481 cgatctcgtc ggctatgccg accggcggcc ccatccgcgc tggcccggcg aggcgcgcat
     3541 cgcggtgcaa tttgcgatca actatgagga aggctcggaa tatacggcga tagacggcga
     3601 cggacgcacg gagcttggcc tcgccgaggc gcccggcgga cgcgtacccg ccggccagcg
     3661 cgacctcgcc ttcgagacga tgtacgaata tggcagccgc gtcgggattt ggcgcctgtt
     3721 ccgcctgttc ggcgaaaggc gattgcccgt cacgatcttc ggatgcgcgc tggcgctcga
     3781 gcgcaatccg cgcgtcgccg aagcgatcgc cggagcgggc tatgacgttt gctgccacgg
     3841 ctggcggtgg gaagagcatt tccggctcgg cgaatccgaa gagcgcgagc gcattgcgcg
     3901 cgccgtcgcc tcgatcgcca aatccgtcgg cgagcgcccg ctcggatggt attgccgcta
     3961 cgggccgagc gagaatacgc gccgcctcct cgtcgaggag ggcggctttc tctacgattc
     4021 cgacgcctat aacgacgagc tgccctactg ggtcgaagtg tcgggcaagc ctcatctcgt
     4081 cgtcccttat acgatggacg ccaatgacgg gaagttcgcg cagccggccg gtttcgggac
     4141 gtcggcggat ttcgagacct atctcaaggc gaccttcgac cagctctatg aagaaggcgc
     4201 ggagaaagcc ggcctgatgt cgatcgggct gcatccgcgg cttgccggcc gtccggcgcg
     4261 cgcccgggcg ttggcgggct ttctcgacta catcctgaag cgcgaccggg tctggatttg
     4321 ccgccgcgtc gacatcgccc gccattggcg cagcgagcac ccggcgcatt gaggagcgca
     4381 gcgaaatgat gagcgacgcg ccttgggaag cctatctgac ggaagccgac cgatccgtcc
     4441 ttgcgcgcgg ccgcttcggg cgccgaatgg gctttggcga aaagccggcg gtgatcgtca
     4501 tcgattgcca gcgttatatg gtcggcgaac gcggcgtcga ggacggccgc tatccatcct
     4561 cctgcggcgc gctgggctgg gcggcggtcg accggatcgc cgcgattctc gacgccgccc
     4621 ggctggtcaa gacgccggtt ttcctcacgc gcttcgcgct cgatccgagc ggcgcggata
     4681 tcggcgtcta tgcccgcaag cgcgatttcc ttcagcgctc ggattggtgc ctcgaaggcg
     4741 ccgaaggcgc cgagctcctg cccgaggtcg gtccgaagcc cggcgacatc gtcttcgtca
     4801 agaagaagcc gagcgctttt cacggaacgc cgctgctcgg ctatttgatc gacaggagaa
     4861 tcgacaccgt catcgtgctc ggcggcgcga ccagcaactg cgtgcgcgcc accgtattcg
     4921 acgccgcctc gtacaatttc cgcgcgatcg tcccggccga cgcggtattc gaccggttgc
     4981 cgatctcgca cgcgatcaat ctcttcgata tggaccggca atttgccgat gtggtgaatt
     5041 ccgacgacgc catcgccgcg ctcgggcgaa ccgcgcgagc agcgagctga tcggcagagg
     5101 acgacaatgc aaggccgctc gattggacgc ggaatgaaaa cggggcgcgg cgacgccgcg
     5161 cctccttgct tgccgctttc cggacaccgc ctgagcgacg gcgcctcacg atgaaaagcg
     5221 ccccaagcga gattcgagcc gagactgatc tcgccgaccg atacggccgg aggaatggcg
     5281 gcgcggccgc cgacccatcc tcggcgggat tcgagagcgt cacgcgcgcc gaccagcaaa
     5341 gacggcgtcg aagaaacctg cggctcctga tctaccgcta cgcggtggtc ctcgccctag
     5401 cggtcttcgt cgtcggcttc tccctgcttc tgccgaggac gtttttcacg ctgggcaatt
     5461 tccgcacgat cgtcagttcg caagcggtgc tgatgattct cgcgcttggg ctgacgctcc
     5521 cgctcaccac cggggaattc gatctgtcga tcggctcgat gctgggctgc gcctcggttc
     5581 tcacggccta cttcaccggc gagctccatt ggcctctggc cctggcgatc ctcctgaccg
     5641 tcttgaccgg cgtcgcgatc ggcgtgctca atgggatttt cgtcgtgcgc gtcggcgtca
     5701 acgcctttat cggcacgctc ggcgcttcga cgatcctgac cggcgtgact ttggcggtgt
     5761 ccggcggcca gatcctcaac aatgtcgccc agccgctggt cgatttcgtc cagtttcagg
     5821 ttttcggcct cgccgcgccg gtctatatcg gcttcctgct ggctttgctc ctctggtact
     5881 tatacgagca tacgccgacc ggccggcatc tctatttcgt cggcgaaggc cgcgaagccg
     5941 cgcgcctcgt cggcctgccc gtcgaccgct tgcggttttc cgctttcgtc gcctccggcg
     6001 tgatcagcgc ggccgccgga atcttcaccg ccgggcaatt gggcgccgcc gatccatcgg
     6061 ttgggccgag ctttctcctg cccgcctacg ccgccgcctt tctcggcgcc acgacgatca
     6121 agcctggacg gttcaacgcc tggggcacgg tcgtcgcgct ttatctcctc gtcaccggcg
     6181 tgaccgggct cgaactcatg gggacctcgt cctgggtgca ggaaatgttc aacggcgcgg
     6241 cgctgctgat cgcggtgacc ttcgcgcggt tcgtggcgcg cgatcaggcc gcataacgac
     6301 tttgaaacgg tgaagacgac agggaggacc ttgagcgatg aaaaactctt ctcgaagaac
     6361 ggcgtcattg ttgcggcgat tgccggtggc ggctcttatc gccggcttct cggcttacgc
     6421 gtcgtcggcg caagccgacg gacttcaaga cgccaaggcc attgtcggaa aggcgatggc
     6481 gaagccgcaa ttcgtcgcgc cgggcgagcc tttcgacatt ggcaagctca agggcaagcg
     6541 catcgccatc atcacctcga ccctcgcggt cccgttcgtc gcgaccattg cgcatggcgt
     6601 cgaggccgcg gccaaagccg ccggcatgga gacgtcgctc ttcgacggaa agggcgacgt
     6661 gtcggaatgg aaccgcggca tggcgcaggc ggtcagccag cacgccgacg gcatcgtgac
     6721 ggtgggcgcc tcgcccgaat tgatgaaggg cccgacagcg gatgcgctca aggcgcatat
     6781 tccggtcgtc gacgcagtga ccgcggacaa gaccgcgcct ctggtgcccg gcacgttctc
     6841 tcatgtcagc atttccttct atcattcggg ccaattgcag gctgacgagg cgatcgccaa
     6901 atcgggcggc aaggcgcatg tcctcatcct tggcgacaac gaattccccg gcgaggtttc
     6961 gcgcgtcgaa gggatgcaga aggaattctc caccctttgt ccggattgca ccgtcactgt
     7021 gcaggatacc caggtcggca atctcggcgt caagctcggt caacagaccc agacgctgct
     7081 gcggcgcagc cccgacatca acattgtcct cgtcacctat gacgcgcagg cgatctatgt
     7141 cgtgcccgcg atcaaggcgg cgaatttttc gaatccgatc gaggtgatcg gcgccgacgc
     7201 cgttccgagc aatctcgact ggattcgtca gggcaatatc caaatcgccg atgtcggcga
     7261 gccggaagtc tggctgggct gggccgcggt ggacgaagtc gcgcgcggca tgctcgccct
     7321 gccgaaggtc gatgagcaaa tcccgcttcg gctcttcgtc aaggagaatc tgaaggatgt
     7381 ttccaatgac gaaaacgagc tatttggcgg cgacttcgtc gcggagtaca aaaagctctg
     7441 gggcttgaaa taggttccgg ctcgacggaa cttgtcgctt atgaccagcg gagacgccgc
     7501 gcttctcgag cttcacggtc tcgacaagag ctttgcgggc gtacgcgtct tgaagtcggt
     7561 cgacctttcc gtccggcgcg gcgagacgca tgcgctgatc ggccagaacg gctcgggcaa
     7621 gtcgaccctg atcaagatcc tgtccggcta tcatgcgccg gacgcgggcc ggatcgtgat
     7681 gcgcggcgct ccggtcccgc tgccgcttcg ccccggcgaa gcggcagcgg ccgggttgcg
     7741 ctttctgcac caggatatcg gcgtggtgcg cacgatgacg gttcttgaaa acctccgcat
     7801 cgggcgcttt cggacttcgc cctatggccg cctgctttgg cgcagcgagc gcgaggccgc
     7861 caaagccatt ctcgcgcagc ttcagctcga catcgacccc gatatgccgg tccgcgacct
     7921 gcccggggcg gagcgggcgc tgatcggctt cgcccgcgcg agccaggaga tggatcacgc
     7981 gcgcggcgaa gcgctcattc tcgacgagcc gacggcgttc ctgcccggac catcggtcga
     8041 aaagctgttc gccgcgatcc gcgaaatcac ccgccgcggc tcggcggtcg tcttcgtcag
     8101 ccatcgtctc gacgaggtga tggcgatcgc cgatcggata tcgatcctgc gcgacggggc
     8161 cttggtggaa accgtggcga aggcggacac caacgagcga aagctggtca cggcgatgct
     8221 cggccgggaa atggacgcgc tctatccaac ccgaccggcc gcgagcggcg agcggatgct
     8281 cggcgtcgaa ggcttaaccg ggcgaatcgc cagcaacgtc gcgttcaccg tccagaaagg
     8341 cgaaatcgtc ggcttgaccg gcctgctcgg catgggccat gacgaagtcc cgtacctgct
     8401 cttcggcgct caacccgcgg tccaaggctc gatcgcgatc gaaggtcgcg ctctcgccga
     8461 attcacgccg cgcaaggcgt tcgaggcggg gatggcgttt cttcccgccg atcgtcagcg
     8521 ccaaagcggc gtccccaagg cgacggttct cgaaaacgtc tcgatgaccg acggccggcg
     8581 tttcgtgaag catggccggc tgcggcgcga tctggagcgg agcgcggtcg aaacggtgct
     8641 cgaacttttc gacgtgcggc cgcccgatcc cgatcggccg cttgcgacgt tgagcggcgg
     8701 caatcagcaa aaggcgctgt tggggaaatg gctgcggacc aagcccaaag tgattctcct
     8761 gcatgagccg acgcaaggcg tcgacatcgg ctcacgaaaa caggtgcttc agatcatcgc
     8821 gcaagccgcg gcggcgggcg ccgcgattgt cgtcgcctcc gcggaatacg aagagctcgc
     8881 ccatacttgc catcgcgttt tgatctttcg gcgcggccgg attgttcgcg aactggccgg
     8941 aaacgatttg accgcggcga gaattgccga gcagtgttat ctgagctgaa gcagccgatc
     9001 ttcccctcgt tgcggtcaca ggttcaggcg ataggcgcgg caagcggtcc ccttgaataa
     9061 agccgctttc tcggcggcgg aggcggccgc gctcaaacgc ttgaacgcat tccataggac
     9121 cgggtagctg acgccccgtt tgtcgacggg gaagttgctc tcgaacatgc atcgctcgac
     9181 gccgaagagc tcaatgcatg tctcgatgta aggacgccag agatccgcca actcggcgga
     9241 cgaggccggc gtcgggcgat gggcggcgtc cagatcgaac ccgaagagat gcattgctaa
     9301 cccgcccaat ttgaccgtga cattgggacg ccgcgcgagc tccgtcatgt tgcgccgcca
     9361 atgggcgaag acctcctgcc gcttgccggc ataggcgccg atcgccaggg gcgcgccgat
     9421 atggtcgaga atgacggtcg tctccggaaa cctcccggca agatcggcga gctccggtat
     9481 ttgcggatgg aaaagccagg agtcgaaaga gaggccatag gcggccaagc gcgcatagcc
     9541 ttcgcggaac gccgcatcga gcagcaggcc tttcgggaaa tcccggctcg accctttgat
     9601 ggtcggatcg gcgtcccaaa ccgaccggcc gcgaacgccg cgaaagcgat cgcccgcggc
     9661 gcgcagatgc gcctcgagaa ccgcgtcgac tttggcgccg agctgaagat cggcatagcc
     9721 gacgatgccg gcgcagaccc gcgccggtcc gtagaggccg ctggcgctca tcgcagcgac
     9781 gccattgacg aactccgttt cgccaagggg cgcgaaagca ggatcgccgc tggcgcgata
     9841 catcgcgtcg cattgcacga agacggtcgc ctcgatcctg tggcccgtct cgatatccgc
     9901 tctcaattcg tcgaaaagat atcgggcgcc gcgatcccag agatgatggt gcggatcgac
     9961 gatcggcagg tcgggctcga taatctcttc gctatactgc ttgagccaga gttcgtcggc
    10021 gccgaataac gcctgatggc gcgcgagcgc cggttcctgc tcagccgtca ttttgctcct
    10081 ccacgaaaaa tcagttccgc cgctgaacgc tgagccgtca aatcgcgagc cgcttaatta
    10141 tgaggggaca tagccggctc gatcaagcga tgcgacgaag cccgtttcgc gccggtcttc
    10201 gggcggctca tccttgaaaa gaccgccagt ggtcacgcgc ttgtttggtt cgcacgagga
    10261 taaacaacca tcccgtccgg ctggatgacg gcgccctccg taagatgggc cggctccaag
    10321 cggcccgccc ccataatatg aaaaacagtc tcgccgcggg cgataagatg atcggcgatg
    10381 atgcgccggt ggcatcgcca ccatacggct tccgaacaca tgatcacgca ccggcgctta
    10441 cgccccactt cgacaagatg ctcaaggccg acgcgaaacc gttctgaaag agcataatcg
    10501 gcgtagttgt ggaag
//
LOCUS       DATGJW010000153         6865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_546360, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000153 DATGJW010000000
VERSION     DATGJW010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6865
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_546360"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..418
                     /locus_tag="VKU03_04640"
     CDS             <1..418
                     /locus_tag="VKU03_04640"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLJ70580.1"
                     /translation="SVLEIGTGAGYATALVSQLARQVLTIERCQSLALEAATRLESFG
                     ATNVRVAWADALADSAPAGPFDRILVHALIEEPLRQFTDRLAATGAIVAARLAPSGRG
                     QRIVKIVRLAEGGFRAEEIGPARSLAPLAAGLSRGL"
     gene            597..1772
                     /locus_tag="VKU03_04645"
     CDS             597..1772
                     /locus_tag="VKU03_04645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan DD-metalloendopeptidase family
                     protein"
                     /protein_id="HLJ70581.1"
                     /translation="MLGAWLAGCSSDSTRFSDAFSLSDGTPTPGATIGAASPTPNANY
                     AAAAHPSPVAVSSLPAPAPTSTGSIRPTTTQPITGFNGGWSTVGGSPIVLADGENLET
                     ISRRYGVPQSALLRANGLTSASEVHPGARLIVPVYNAGAKTAAAEPKAREAETAERRD
                     SRAKAEKSAKESKKAKDKEADASEHKKQEKAKVAEAKAGKAEKAEKPAKAEKTEKAAT
                     VKTEPARKQAEAKPDAPAKKTAATDQTPTGSVHPEETTAAMTSAQADAAGVTPEFRWP
                     ARGRIIQGFKVGGNDGINIAVPEGTSVKAAESGVVAYAGNELKGYGNLILIRHPNGFV
                     TAYANNGELEVKRGEQVKRGQIIAKSGQSGNVSSPQLHFELRKGSTPVDPTSYLAGL"
     gene            complement(2086..2604)
                     /locus_tag="VKU03_04650"
     CDS             complement(2086..2604)
                     /locus_tag="VKU03_04650"
                     /inference="COORDINATES: protein motif:HMM:NF020496.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HLJ70582.1"
                     /translation="MKFRSIIQLNKINPYVLVSADRATRLKQGWRKPMPVLVQVNGEP
                     NPPWRVNMMPVGDGSFYLYLHGAVRKASTTAVGDEVEISLSFDKSYRGGPIHEAPAEF
                     AARLSAEPKAQAAWRKLPPSRQKEILRYLATLKSDAAKTRNVERAIRVLSGSKERFLA
                     RDWNAASSVPSR"
     gene            2820..3716
                     /locus_tag="VKU03_04655"
     CDS             2820..3716
                     /locus_tag="VKU03_04655"
                     /inference="COORDINATES: protein motif:HMM:NF016648.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FecR family protein"
                     /protein_id="HLJ70583.1"
                     /translation="MTPEQTGSGLSRRTCASRRRRALSSVALVAALVAGVSSSRAEQG
                     IGGAEIVINNVTGALVSGRDVHVAQGDNVFRDEAVRTAPDSSAKLVLRDNTNLSLGPS
                     SSITLDRFVYGGAGRRGAIAVNLVKGALRFATGDADKQAYKITTPTATLGVRGTVLKI
                     DATAAKTIVFLEEGAVIVCTRRAGRHCIELTAPGQTAVVTVTQVGLEGPTSPGETGGT
                     NTGRLGVSPGPSAAPPGPAPSPSGPSNSGPGKGNPGNSGGSTGHGDGDGDGDGDGDGN
                     GNGHGHASGNGPGHGHGGGNGH"
     gene            3831..6053
                     /locus_tag="VKU03_04660"
     CDS             3831..6053
                     /locus_tag="VKU03_04660"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017076.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ70584.1"
                     /translation="MGRLKGYRLYLAAVLVVYVDMAVLAAMLPAARADLQNIVFDQYQ
                     RWRPRVRESDLVRIVDIDDESIRRLGQWPWPRQAVARLVQILSQGRPAAICFDILFSE
                     AERSGFVKSRGAAQRPLAVASATAAPEAASEGDAALAGAIAGRPVVLSELLMNGHFAG
                     AVPTKSGFAIVGDDPSQNLPRLTGVIAPLDAFAEKAAGIGFVNWQADDDRVVRRVPLL
                     MTVNGHMQPSFAIESLRVAQDASTYILKSAAGARDDAFASTSGLAAIKVGDLVVPTQP
                     AGDIRAYFAKADPRLSTPAWKLFESGADLSGFAGKIVVVGASAAMLSDVVATPLNPST
                     PGVEAQAQLIEQMLDGASLLRPDWAFGAELLAGTLISLMLVMALPKISALATALLGAL
                     AVAALGVVSGLSFVKYGLLLDPVTPSLSSGAVFLAGALALYGQKRQQLTEIRSLFGRF
                     VSAAVVERLADRPEAIKLGGEQRRLTLMFCDLRSFTTLAETFNAIELTSFLNEYLTPM
                     TDIVLARMGTIDKYMGDAIMAFWNAPLDDAAHAAHAARAALDMRGALAELNRRWAARG
                     SESDGSVRHLKFGVGLNTGECCVGNLGSTRRLDYSAIGDEVNVASRLEGACKIFQVDI
                     IGSETVRAEAPDFAWLEIDSVLVKGKTRPVGLYTLAGDKALARSDEFADLTRLHAAML
                     AAYRNRDFAGAISMAKEAAARAPDAVRGLYSYNLRRFAQLAETVQDPDWRPLIALDEK
                     "
     gene            complement(6065..>6865)
                     /locus_tag="VKU03_04665"
     CDS             complement(6065..>6865)
                     /locus_tag="VKU03_04665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70585.1"
                     /translation="GANEQVSGCDCGGGFASIGSFSLGSHGRWSLNDSLTLLAGAAFE
                     QYYQDGANVRAAPIVAASLRYDPPNWGKSRPFLEFGGAASPYIDTTYTRYYTNGLTPA
                     QGVGSAVDRSISVFGRIGWVDRLTPIDEAAVFTDLVRGWQQSGGYTEAANALNPFPAT
                     VSTGVDRQDVVRFGATYTHLLFGNLEANINGAIAYGFDNEFGARVNVVSFGSVAPYPL
                     LNSAWTEFGGRLGYRFSRNLVVDAFLIGTLGGEIGPTLHAGLGVRYAF"
BASE COUNT         1218 a         2234 c         2263 g         1150 t
ORIGIN      
        1 cagcgtttta gagatcggca cgggggcagg ctacgccacc gcgctcgtct ctcaactcgc
       61 ccgccaggtg ctgacgatcg agcgctgcca gtccttggcc ttggaggcgg cgacgcggct
      121 cgaatcgttc ggcgcgacca atgtccgggt cgcctgggcg gacgctcttg ccgacagcgc
      181 gccggccgga ccgttcgacc gcattctcgt ccatgcgctg atcgaagagc ctctgcggca
      241 attcaccgac cggctcgccg cgaccggcgc gatcgtcgcc gcccgcctcg cgccaagcgg
      301 gcgcggacaa aggatcgtga agatcgtccg gctcgcggaa gggggatttc gcgccgagga
      361 gatcgggccg gcgcgctcgc tggcgccgct cgccgccggc ctgtcgcgtg ggctttaggg
      421 cgcaaagccg gcgaaaaatc gctcaatttt gacgaaaacc attcaccctc aaccttccct
      481 taacctcaat acggttaaat ccgccaatca tcagtgcgtt cgggtgggtt aatccgatga
      541 gtgagagtgg ggcgttcaat ttggcgcgcg tctcagcgcg cctcgcggcg gctggcttgc
      601 tgggagcgtg gctcgccggc tgttcgtcgg attcgacgcg cttctccgac gcgttctcgc
      661 tgagcgacgg aacgccgacg ccgggcgcga cgatcggcgc cgccagcccg actcccaacg
      721 ccaattacgc ggccgcggcg catccaagcc cggtcgcggt ctcgtcgctt cccgcgcctg
      781 cgccgacttc gaccggctcg atccggccga ccacgactca gccgatcacc ggcttcaatg
      841 gcggctggag cacggttggc ggctcgccga tcgtccttgc cgacggcgaa aatctcgaga
      901 cgatttcgcg ccgctacggc gtgccgcaat cggccttgtt gcgcgccaac ggtttaacca
      961 gcgcgagcga agtccatcct ggggcgcggc tgatcgtgcc agtctacaat gccggggcca
     1021 agaccgccgc ggccgagccg aaggcccgcg aagccgaaac ggccgagcgc agggactcca
     1081 gggccaaggc tgagaagtcg gccaaggagt cgaagaaggc caaggacaag gaggccgacg
     1141 cttccgagca taaaaagcag gagaaggcca aggtcgccga ggcgaaggcc ggaaaggccg
     1201 agaaagccga gaagcctgcc aaagcggaga agaccgaaaa ggccgcgacc gtgaagaccg
     1261 agccggccag gaagcaggcc gaggccaagc cggacgcgcc ggccaagaaa accgccgcga
     1321 ccgatcagac cccgacgggc agcgttcatc ccgaagagac gaccgcggcg atgacgagcg
     1381 cgcaggccga cgcggcgggc gtcacgccgg aattccgctg gccggcgcgc gggcggatca
     1441 ttcagggttt caaggtcggc ggcaatgacg gcatcaacat cgctgtgccg gaaggcacct
     1501 cggtcaaggc ggccgaaagc ggcgtcgtcg cctatgccgg caacgagctg aaaggctatg
     1561 gcaacctcat cctgatccgc cacccgaacg gcttcgtcac cgcctacgcc aacaatggcg
     1621 agctcgaagt caagcgcggc gagcaggtta agcgcggcca gatcatcgcc aaatccggcc
     1681 agagcggcaa cgtctcctcg ccgcaactgc atttcgaatt gcgcaagggt tcgaccccgg
     1741 tcgatccgac cagttatctc gcggggcttt aggaaagccg cgggagccga cgtccagtcc
     1801 tctggaccaa tcccgcagat tcgccgcgag acacgttgaa tcggcgggaa taaaatgaag
     1861 gcggcgggtc ccaggaaagg cccgccgcct ttttccttta agaattatgg tgacagtgca
     1921 ctcaattcca gcagtgatgc gagtgtgctg cggttaagca gctcgcggcg cgctgtcgac
     1981 tagatgcaac ctcgccatga tcacagcctt tcgcccgccg gtggaaattt agtactgtca
     2041 cggtaatgcc cgtaatgtgc gggaagagtt tcggaagagc ggcgttcagc gcgaaggtac
     2101 agaagacgct gcgttccaat cccgcgccag gaagcgttct ttcgatcccg acagaaccct
     2161 gatcgctctt tcgacgttgc gggtttttgc agcgtcggat ttcaacgtcg caagatagcg
     2221 gagaatttcc ttctgtcggc tcggcggaag cttccgccac gccgcctgcg ccttgggctc
     2281 ggcgctgagc cgcgcggcga attcggcggg cgcctcatgg atggggccgc cgcgatagct
     2341 tttgtcgaaa gatagcgaga tttcgacctc gtcgccgacg gcggtcgtcg aggctttgcg
     2401 aacagcgcca tgaagataga gatagaaact gccgtcgccg accggcatca tgttgacgcg
     2461 ccatggcggg ttgggctcgc cattgacctg cacaaggaca ggcatcggtt tccgccagcc
     2521 ctgtttcagg cgcgtggcgc gatcggcgct gacgaggacg taagggttga tcttattgag
     2581 ctgaatgatc gatcgaaact tcatggcgag gctgacggga aaagcgaaac gaattgaggc
     2641 ccattttctt cagatcgcgg gactggcgcc aagcctacca tccgatcgag ctactccgaa
     2701 agcgcaaggc gacgcgttgg aacatgcgag cttcacccat ctcttgccgt tgcgggcgcc
     2761 gcgtcgtttt cactaagctt taaatcattg ttctccaggt cgggcgtcgg agagttcggt
     2821 tgacgccgga gcaaacgggc agcggtcttt cccgccgaac ttgcgccagt cgccgtcgcc
     2881 gcgcgctttc gagcgtcgcg cttgtcgcgg cgcttgtcgc gggcgtctcc tcgtcgcgcg
     2941 ccgagcaggg cattggcggc gcggaaatcg ttatcaacaa tgtgacgggc gcgctcgttt
     3001 ccgggcgcga tgttcacgtc gcgcagggcg acaatgtctt tcgcgacgag gcggttcgga
     3061 cggcccccga cagttccgcg aagctggtgc ttcgcgacaa caccaatctg tcgcttggac
     3121 cgtcctcctc gatcaccctc gatcgcttcg tctatggcgg cgccgggcgg cgcggcgcga
     3181 tcgccgtcaa tctcgtcaaa ggcgctctgc gcttcgccac cggcgacgcc gacaagcagg
     3241 cctataagat caccacgccg accgccacgc tcggcgtgcg cggcacggtt ttgaagatcg
     3301 acgcgactgc cgcgaagacg atcgtttttc tcgaagaggg cgcggtcatc gtctgcaccc
     3361 gccgcgccgg gcgccattgc atcgagctga cggcgcccgg ccagacggcg gtggtcaccg
     3421 tcacccaagt ggggctggaa ggtccgacca gtcctggcga aacgggcggg accaataccg
     3481 gacgccttgg cgtctcgccc ggcccaagcg ccgcgccgcc tggccccgct ccgagcccaa
     3541 gcgggccgag caatagcggc cccggaaagg gcaatccggg aaatagcggc ggctcgaccg
     3601 gccatggcga tggagacggg gatggcgacg gcgatgggga cgggaatggg aatggacacg
     3661 gccacgcctc cggcaatggc cctggccatg gccatggggg cggcaacggc cattgagcct
     3721 cggctcgcgc cgcccgccgg ttttgccggc ggaccgccgt aacgagggcg agcgtggcgc
     3781 ctggcgatca gccacgccgc cggcgctgat cacgcgagga agcgtcggcg atgggtagat
     3841 tgaaaggcta ccgcctctac ttggccgccg ttctcgtcgt ctatgtcgat atggcggtcc
     3901 tcgccgcgat gctgcccgcg gcgcgcgccg acctgcaaaa catcgtcttc gaccagtacc
     3961 aacggtggcg cccgcgcgtc cgcgaaagcg acctcgtacg aatcgtcgat attgacgacg
     4021 aatccattcg gcgactcggg caatggccgt ggccgcgtca ggctgtcgcc cgtctcgtcc
     4081 aaattctttc gcaaggccgc ccggcggcga tctgcttcga tattctcttt tcggaagccg
     4141 aacggtccgg cttcgtgaaa tcgcgcggcg ccgcgcagcg gcctctcgcc gttgcgagtg
     4201 cgacggcggc gccggaagcg gcgagcgaag gcgatgcggc gctggcgggc gcgatcgccg
     4261 gccggccggt cgtgctgagc gagctcttga tgaacggcca tttcgccggc gccgttccga
     4321 cgaaaagcgg cttcgccatt gtcggcgacg acccgagcca aaatctgcct cgacttaccg
     4381 gcgttatcgc gccgttggac gcatttgccg agaaagcggc cgggatcggc ttcgtcaatt
     4441 ggcaggccga tgacgatcgc gtggtgcggc gcgtaccgct gcttatgacg gtcaatggcc
     4501 atatgcagcc gagctttgcg atcgagtcgc tgcgggtcgc gcaagacgcg tcaacctata
     4561 ttctcaaatc cgccgccggc gctcgcgacg acgcgttcgc ctcgacgtcc ggcctcgccg
     4621 cgatcaaggt cggcgacctc gtcgtaccga cgcaaccggc cggcgatatt cgcgcctatt
     4681 tcgcgaaggc cgatcctcgc ctgtcaacgc ccgcctggaa gctgttcgag agcggcgccg
     4741 atctttccgg tttcgccgga aagatcgtcg tcgtcggcgc cagcgccgcg atgctgtcgg
     4801 atgtcgtcgc gacgccgctt aacccttcga cgcccggcgt cgaggcgcaa gcgcaattga
     4861 tcgagcagat gctggacggc gcgagcctgc tgcgccccga ctgggctttc ggcgccgaac
     4921 tgctcgccgg aacgctgatt tccctcatgc tcgtcatggc gctgccgaaa atttccgcgc
     4981 tcgcgacggc gctgctcggc gccttggccg tcgctgcgct tggcgtggtc agcggcctca
     5041 gcttcgtcaa atacgggctg ttgctcgacc cggtcacgcc cagcctttcc tccggcgccg
     5101 tgtttctcgc cggcgctttg gcgctttacg ggcaaaaacg ccagcaattg acggaaatac
     5161 gatcgctgtt cggccgattt gtctcggcgg cggtcgtcga gcggctggcc gaccgcccgg
     5221 aggcgatcaa actcggcggc gagcagcgcc ggctcacgtt gatgttctgc gacctgcggt
     5281 cgtttacgac gctggcggag acgttcaacg caatcgagct cacctcgttc ttaaacgaat
     5341 atctcacgcc gatgaccgac atcgtcttgg cgcggatggg cacgatcgac aaatatatgg
     5401 gcgacgcgat catggcgttc tggaacgcgc cgctggacga cgcggcgcac gcggcccatg
     5461 cggcgcgcgc cgcgctcgac atgcgtggcg cgctcgccga gctcaatcgc cgctgggcgg
     5521 cgcgcggctc ggaatcggac gggtcggttc gccatttgaa attcggcgtc ggcctcaaca
     5581 cgggcgagtg ctgcgtcggc aatctcggat cgacgcgacg gctcgactat tcggcgatcg
     5641 gcgacgaggt gaacgtcgcc tcgcgtctcg aaggcgcgtg caagattttc caggtcgaca
     5701 tcattggcag cgaaacggtc cgcgccgaag cacccgactt cgcctggctc gagatcgatt
     5761 cggtgttggt caaaggcaag acgcgcccgg tgggtcttta cacactcgcc ggagacaagg
     5821 cgcttgcccg aagcgacgaa ttcgcggacc tcacccgcct gcacgcggcg atgctcgccg
     5881 cctaccggaa ccgggacttc gccggggcca tttccatggc gaaggaggcc gcggcccgcg
     5941 cgccggacgc ggttcggggc ctctactcct ataacctgcg ccgtttcgcc caattggccg
     6001 aaaccgtgca ggacccggac tggcgcccgc taatcgcgct cgacgaaaaa tagcggtccc
     6061 gatctcaaaa cgcgtagcgc acgccgaggc ccgcatgcag cgtcgggccg atctcgccgc
     6121 cgagcgtgcc gatcaggaag gcgtcgacca ccagattgcg cgagaaccga tagccgaggc
     6181 gcccgccgaa ctcggtccac gcggagttta gcagcgggta tggggcgacg gatccgaagg
     6241 agacgacatt gacccgcgcc ccgaactcgt tgtcgaaccc ataagcgatc gcgccgttga
     6301 tattggcctc aaggttgccg aacaggaggt gggtgtatgt cgcgccgaag cgcactacgt
     6361 cctgcctgtc gacgccggtg ctcaccgtgg cggggaaggg attgagcgcg ttcgccgctt
     6421 cggtgtagcc gcccgattgc tgccagccgc ggaccagatc ggtgaacacg gctgcctcat
     6481 cgatcggagt cagccggtcg acccagccta tgcgcccgaa caccgagatc gaccggtcga
     6541 ccgccgaccc gaccccctgc gccggagtga ggccgttggt gtagtagcgg gtgtaggtcg
     6601 tgtcgatata gggcgaagcc gcaccgccga actcgaggaa cgggcggctc ttgccccagt
     6661 tcggaggatc gtagcgaagc gaggcggcga cgatcggggc ggctcgcaca ttggcgccgt
     6721 cctgatagta ttgctcgaaa gcggcgcccg ccagaagggt caggctgtcg ttgaggctcc
     6781 atcgcccgtg agagccgagc gaaaaggagc cgatcgaagc aaagccgccg ccgcaatcgc
     6841 agccgctcac ctgctcgttg gcgcc
//
LOCUS       DATGJW010000154         8472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1478895, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000154 DATGJW010000000
VERSION     DATGJW010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8472
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1478895"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..486)
                     /locus_tag="VKU03_04670"
     CDS             complement(<1..486)
                     /locus_tag="VKU03_04670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLJ70586.1"
                     /translation="MASGDFKMFGYPFRKSGQEHSGQPPALQNGRRQPKIAVVLGAGA
                     ARGWAHIGLLQEFVAMGVTPSIVVGASIGAVVAGCYAGGRLGELEAFSRSLTKRSVFG
                     LMDVSFNGGGLIAGARLRGRLENAFAGVNIEDLPISFASVATEIGTGNEIWLTRGSLV
                     EA"
     gene            complement(595..993)
                     /gene="hisI"
                     /locus_tag="VKU03_04675"
     CDS             complement(595..993)
                     /gene="hisI"
                     /locus_tag="VKU03_04675"
                     /EC_number="3.5.4.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004635 - phosphoribosyl-AMP
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-AMP cyclohydrolase"
                     /protein_id="HLJ70587.1"
                     /translation="MSAPAVDKKALEEGADFLPQFSPDGLVACITVDDASGDVLMFAH
                     MNREALEKTLATGIVHYWSRSRQALWRKGDTSGQIQTLVELRVDCDQDALLARVRVGG
                     DGGACHTGRRSCFYRRIAITAAGPRLESPP"
     gene            complement(999..1628)
                     /gene="folE"
                     /locus_tag="VKU03_04680"
     CDS             complement(999..1628)
                     /gene="folE"
                     /locus_tag="VKU03_04680"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003934 - GTP cyclohydrolase I activity
                     [Evidence IEA];
                     GO_process: GO:0042559 - pteridine-containing compound
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0046654 - tetrahydrofolate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I FolE"
                     /protein_id="HLJ70588.1"
                     /translation="MDDFVTLKPAPRRAAPALRARPSREDAEAAVRTLIAWAGDDPDR
                     EGLAETPRRVVKSYEELFSGYREDPDAALSRVFKEVRGYSDIVLVRDIPFVSHCEHHM
                     APFLGKAHIGYYPRNGVVGLSKLARIVDTFARRLQTQEALTAQIAEAVDNALKTRGVA
                     VLVEAEHMCMSIRGVRKHGVSTATTQFTGLFQEDSNEQMRFLTLLRGAR"
     gene            1774..2229
                     /locus_tag="VKU03_04685"
     CDS             1774..2229
                     /locus_tag="VKU03_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HLJ70589.1"
                     /translation="MLDSIYNKRILELAGDIPRLGRLPAPDATARAHSKLCGSTVVVD
                     LCLRDGRVSDFAHDVKACALGQASSSLMARHIIGATPDELLALRDAMRRMLKENGPPP
                     SGKWADFAALEPVRDYKARHASTMLTFEAVADALGQIAAKRQVEAAAAK"
     gene            2235..2579
                     /gene="yidD"
                     /locus_tag="VKU03_04690"
     CDS             2235..2579
                     /gene="yidD"
                     /locus_tag="VKU03_04690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HLJ70590.1"
                     /translation="MSLAPRRAAHGAIRAYQLTLSSILGRRCRYLPTCSDYADEAICR
                     HGLWAGGWMGLARICRCHPYGGSGYDPPPQTAPEGASWLRPWRYGAWRTPESASKPAQ
                     AGEASGLPFSTT"
     gene            complement(2537..2935)
                     /locus_tag="VKU03_04695"
     CDS             complement(2537..2935)
                     /locus_tag="VKU03_04695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLJ70591.1"
                     /translation="MLTRRGFATCALCALSGFAATAAEAAGPAGLKRTILQRTDGPAP
                     GYETLVVAIEIEPGFQVARHTHPGIESSYVIEGETELSMDGEKPRMVGPGQSFQIPTG
                     VPHSAKNGDKTARLAAVYVVEKGKPLASPA"
     gene            3089..5068
                     /gene="thrS"
                     /locus_tag="VKU03_04700"
     CDS             3089..5068
                     /gene="thrS"
                     /locus_tag="VKU03_04700"
                     /EC_number="6.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004829 - threonine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006435 - threonyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="threonine--tRNA ligase"
                     /protein_id="HLJ70592.1"
                     /translation="MINITFKDGASRAFEPGLSGLDIAKSISPSLAKRSVAMILDGKL
                     SDLSDKIAQNASVEFITREDPRALELIRHDAAHVLAEAVQSLWPGTQVTIGPVIENGF
                     YYDFFRNEPFTPEDFPAIEKRMREIIARDKAFTKEVWSREQARDFFEKKGEAFKVELV
                     DAIPPGEDLKIYRQGEWLDLCRGPHMTSTGKVGDAFKLMKVAGAYWRGDSKRPMLSRI
                     YATAFANRAELDAYLKQLEEAERRDHRRLAREMDLFHFQEEGPGAVFWHPKGWTLFQT
                     LIAYMRRRLAEDYQEVNAPQVLDKHLWEISGHWGWYRENMFMARPAGDDAEAEDERVY
                     AIKPMNCPGHVQIFKNGLRSYRELPLRFAEFGIVHRYEPSGALHGVMRVRAFTQDDAH
                     IFCTEEQLAAECHKINELILSVYHDFGFDKIVVKLSTRPEKRVGTDAMWDHAEDVMRR
                     VLEEIKARHGNVVTTMINPGEGAFYGPKFEYVLRDAIGRDWQCGTTQVDFALPERFGA
                     FYIAANSEKTTPVMVHRAICGSMERFIGILLEHHAGHLPLWLAPLQIVVCTITQDADD
                     YAMEIVAKARKRGLRVEGDLRNEKISYKVREHSLAKVPVLLVAGKKEAGERTISIRRL
                     GSNEQKAMSLDAALAALVDEAVPPDLAPARRAAAE"
     gene            5077..5883
                     /locus_tag="VKU03_04705"
     CDS             5077..5883
                     /locus_tag="VKU03_04705"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HLJ70593.1"
                     /translation="MAAVLGIDAAWTAKNASGYALIGKSGGAWRLRAAAPDLCAFARK
                     CRLDGAGPGMDGAIACAKNLLGRLPDLIAVDMPLSKQQITGRRASDIGVSRRFGAAKC
                     ATHSPSAERPGKVSGAFRAACEKAGYALVVSAGALPALSLAEVYPHPALLRLMRAGER
                     VPYKTGKTKIYWPLASCDERLARVTRSLGAIVDALESHIAGVARETAPLFAAAENFAG
                     LKPAEDMIDAIVCAWIGTTILAGEAEPIGDADSAIWIPDPLKPTLLAGAA"
     gene            6041..7075
                     /locus_tag="VKU03_04710"
     CDS             6041..7075
                     /locus_tag="VKU03_04710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015293 - symporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bile acid:sodium symporter family protein"
                     /protein_id="HLJ70594.1"
                     /translation="MRGVPLMRWVASFKPDNFTLAIAAAVVIASFLPAIGLAATVLHY
                     VTIFAIALLFFLHGARLSREAVLAGALHWGLHLIVLCATFVLFPVLGLILRRALGPVL
                     PDSLLVGLLFLCLLPSTVQSSIAFTSIAHGNVAAAVCSASLSNLLGIFITPALVALLM
                     NLKGGVSLHAVLDIVLQLLAPFLVGHFLRPWIGGFVARHRYALGYVDRGSILLVVYLA
                     FSEAVANGLWHKLGASDIAKLLIVCAALLCAVLLATVTLARRLGFSRPDEIVIVFCGS
                     KKSLASGVPMAGVLFPLAQVGMIVLPLMLFHQIQLMVCAALAKRYAASAALETVLAAK
                     PEAGGREAAG"
     gene            complement(7161..7409)
                     /locus_tag="VKU03_04715"
     CDS             complement(7161..7409)
                     /locus_tag="VKU03_04715"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII small subunit"
                     /protein_id="HLJ70595.1"
                     /translation="MTDTPDDLAKLPFEAALAELESIVDKLEKGAAPLEESIKIYERG
                     EALKAHCDRLLREAEMRIEKITLGADGKPKGVEPLDPK"
     gene            complement(7468..7935)
                     /locus_tag="VKU03_04720"
     CDS             complement(7468..7935)
                     /locus_tag="VKU03_04720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007092109.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLJ70596.1"
                     /translation="MPSEPLDERDRAILRALMRQGRLANADLAERVNLSPSACLRRVR
                     MLEESGLIAGYVMLLDPARAGLPGAAFVFVTLDQQGRAALDRFEAEARRHAEIIECYL
                     LAGSADYLLRIAYRDAADFERIHTDILTQLPGVSRVQSTLALRTVKKTTELPI"
     gene            8075..>8472
                     /locus_tag="VKU03_04725"
     CDS             8075..>8472
                     /locus_tag="VKU03_04725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008548105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine dehydrogenase"
                     /protein_id="HLJ70597.1"
                     /translation="MLIGVPKEIKDNEFRVGLTPSSVAELVHHGHQVWVERSAGIGSG
                     LPDSEYAQAGAILVDGQDDIFARAEMIVKVKEPLAAEREKLRRGQVLFTYLHLAPDLE
                     QTKDLIASGATCIAYETVTSPHGGLPLLTP"
BASE COUNT         1422 a         2718 c         2787 g         1545 t
ORIGIN      
        1 cgcctcgacc agcgagccgc gggtcagcca gatctcgttg cccgtgccga tctcggtggc
       61 gaccgaagcg aagctgatcg gcaggtcttc gatattgacc ccggcgaaag cgttttcgag
      121 ccggccgcgc agtctcgcgc cggcgatcag gccgccgccg ttgaaagaaa cgtccatcag
      181 cccgaaaacg ctgcgcttgg tcagcgaccg gctaaaggct tcgagctcgc cgaggcggcc
      241 gccggcatag cagccggcga caacggcgcc gatcgaggcg ccgaccacga tgctgggcgt
      301 gacccccatt gcgacgaatt cttgcaggag gccgatatgg gcccagccgc gcgccgctcc
      361 ggcgccgaga accacggcta ttttcggctg gcggcggcca ttttgaaggg ccggcggctg
      421 accggaatgt tcctgaccgc ttttacgaaa cggatagccg aacatcttga agtcgcctga
      481 cgccacgagc tcctcgaccg gcgggcaaat ggggagcgat ggtcatagta aggcgaagtt
      541 gtcgccgttt cattaacgaa aagacaaggg ggaatgttgc agcaggcgtg gagattacgg
      601 gggagattcg agcctcggcc cggcggcggt tatggcgatg cggcgataga agcaggagcg
      661 gcggccggta tggcaagcgc cgccgtcgcc gccgacgcga acgcgggcga gaagcgcgtc
      721 ctgatcgcaa tcgacgcgaa gctcgaccag ggtctgaatt tgtccggacg tgtcgccttt
      781 gcgccagagc gcctggcgcg agcgcgacca atagtgaacg atcccggtgg cgagcgtctt
      841 ttcaagggcc tcgcggttca tatgagcgaa catcagcaca tcgccgctcg catcgtcgac
      901 cgtgatgcag gcgacaaggc cgtccggcga gaattgcggc agaaagtccg cgccttcctc
      961 caacgccttt ttgtcgacag ccggcgcgct catcggcgtc accgcgcgcc gcgcagcagc
     1021 gtcagaaaac gcatctgctc gttcgagtcc tcttgaaaca gcccggtaaa ctgcgtggtg
     1081 gcggtcgaga cgccgtgctt gcgcacgccg cggatcgaca tgcacatatg ctcggcctcg
     1141 acgagcaccg cgacgccgcg cgtcttgagc gcattatcga cggcttcggc gatttgcgcc
     1201 gtcagcgcct cctgggtttg cagccggcgc gcgaacgtgt cgacgatccg cgccagtttc
     1261 gacaggccga ccacgccgtt gcgcggataa tagccgatat gcgccttgcc gagaaacggc
     1321 gccatatggt gttcgcaatg cgaaacgaag ggaatatcgc gcaccagcac gatatcggaa
     1381 tagcctcgca cctctttgaa gacgcgcgaa agcgcggcgt cggggtcctc gcgatagccc
     1441 gagaacagct cctcatagga tttgaccacc cgccgcggcg tctcggcgag gccctcgcgg
     1501 tcgggatcgt cgcccgccca ggcgatgagg gtgcgcaccg ccgcttccgc gtcttcccgg
     1561 ctcgggcggg cgcgtagggc gggggcggcg cgtcgaggcg ccggcttcag cgttacgaaa
     1621 tcatccataa aagaggcctt tcaggcgtct tgggcttgtc tagcgctgct ctcgaagccc
     1681 cgcgcggcgc cctcgtcatt gattggtccg gcgcttatat aagggacggg ctggccttcg
     1741 ccaccggctc ggagccattt ttccgttact gacatgctgg acagcattta caacaagcgc
     1801 attctggaat tggctggcga cattcctcgc ctgggccgcc ttccggcccc ggacgccacc
     1861 gcacgggcgc attccaagct ttgcggctcg accgtcgtgg tcgatctttg cctgcgcgac
     1921 ggacgggtca gcgatttcgc tcatgacgtg aaggcgtgcg cgctcggcca agcctcgtcg
     1981 tcgctgatgg cgcgccacat tatcggcgcg acgccggacg aattgcttgc gctgcgcgac
     2041 gcgatgcggc gcatgctgaa ggagaacggc ccgccccctt cgggcaaatg ggcggatttc
     2101 gccgcactcg agccggtgcg cgattacaag gcccggcacg cctcgaccat gctcaccttc
     2161 gaagcggtgg ccgacgctct cggccagatc gcggcgaagc gccaagtcga ggccgcggcg
     2221 gcgaaatgag cgcgctgagc ctggcgccgc gccgcgccgc ccatggcgcg atccgggcct
     2281 accaactcac cctctcttcg atccttggcc ggcgctgccg ctatctgccg acttgctccg
     2341 actatgccga cgaggcgatc tgccgccacg gcctctgggc cggcggctgg atgggcctgg
     2401 cgcgaatctg ccgctgtcat ccctatggcg gctcgggcta cgacccgccg ccgcagaccg
     2461 cgcctgaagg cgcttcctgg ttgcggccgt ggcgctacgg cgcgtggcgg acgccggagt
     2521 cggcctcgaa acccgctcag gctggcgaag cgagcggctt gcctttttcg acgacataga
     2581 ccgctgcgag gcgggcggtc ttatcgccgt tcttggcgct atgcggcacg cctgtcggga
     2641 tctggaagct ctggccgggg ccgaccatgc gcggcttctc gccgtccatc gacaattccg
     2701 tctcgccttc gatgacgtag ctcgattcga tgccgggatg cgtgtgccgg gcgacctgga
     2761 agcccggctc gatctcgatc gcgacgacca aagtctcata gccgggcgcc gggccgtccg
     2821 tgcgctgcag gatcgtgcgt ttgagcccgg cgggaccggc cgcctccgcg gccgtggcgg
     2881 cgaagccgga aagcgcgcag agcgcgcaag tcgcaaaacc tcggcgggtc agcatgtttc
     2941 cctccctttg gctgccgcga tcatggcgag ggcgcggcaa acgcgcaagg ctcgcgcgaa
     3001 gccgcgccat tgctcttgct cggcgccgcc gctatatcgc gcgcgctgct tacgccgatt
     3061 ggtgtcggcg agtgagcaag gagcgacgat gatcaatatt actttcaagg acggcgcgtc
     3121 gcgcgccttc gagcccggcc tttccgggct cgacatcgcc aaatccattt ccccatcgct
     3181 cgccaagcgc tccgtcgcga tgattctcga cggcaagttg agcgatcttt ccgataagat
     3241 cgcgcaaaac gcgagcgtcg agttcatcac ccgcgaggac ccgcgcgcgc ttgagctgat
     3301 ccgccacgac gccgcgcatg ttctcgccga agccgtgcag tctctctggc cgggaacgca
     3361 agtcacgatc gggccggtga tcgagaacgg cttctattac gacttcttcc gcaacgagcc
     3421 gttcacgccc gaggacttcc cggcgatcga gaagcgcatg cgcgagatca tcgcgcggga
     3481 caaggcgttc acgaaagagg tctggagccg cgagcaggcg cgcgatttct tcgagaagaa
     3541 gggcgaagcc ttcaaggtcg aattggtcga cgcgatcccg cccggcgagg acttgaaaat
     3601 ctaccgccag ggcgaatggc tcgatctctg ccgcggcccg catatgacct cgaccggcaa
     3661 ggtcggcgac gcgttcaagc tgatgaaggt cgccggcgcc tattggcgcg gcgattccaa
     3721 gcggccgatg ctgtcgcgca tctatgcgac cgccttcgcc aatagggccg agctcgatgc
     3781 gtacctcaag cagctcgaag aggccgagcg gcgcgatcat cgccggctgg cgcgcgaaat
     3841 ggacctcttc cactttcagg aggaggggcc gggcgcggtc ttctggcacc ccaaaggttg
     3901 gaccctgttc cagaccttaa tcgcctatat gcgccggcgg ctcgccgagg attatcaaga
     3961 ggtcaacgcg ccgcaggtgc tggacaagca cctctgggag atttccggtc attggggttg
     4021 gtaccgcgag aacatgttca tggcgcggcc ggccggcgac gacgccgaag ccgaggatga
     4081 gcgcgtttac gcgattaagc cgatgaattg ccccggccat gtgcagatat tcaagaacgg
     4141 cttgcgttcg taccgcgagc tgccgctgcg cttcgccgaa ttcggcatcg tccatcgcta
     4201 tgagccgtcc ggcgccctgc acggcgtgat gcgggtgcgc gccttcaccc aggacgacgc
     4261 gcatattttc tgcaccgagg agcagctcgc tgccgaatgc cacaagatca acgagctgat
     4321 cttgtcggtg taccacgatt tcggcttcga caagatcgtc gtgaagctgt cgactcgccc
     4381 ggaaaagcgc gtcggcacgg acgcgatgtg ggaccatgcc gaagacgtga tgcggcgggt
     4441 gctggaggag atcaaggcgc gccacggcaa tgtcgtgacg accatgatca atccgggcga
     4501 gggcgccttt tacgggccga aattcgaata tgtcttacgc gacgcgatcg gccgcgactg
     4561 gcaatgcggc acgacccagg tcgatttcgc gctgccggag cgcttcggcg ccttctatat
     4621 cgcggccaat agcgaaaaga cgacgccggt catggtccat cgggcgattt gcggctcgat
     4681 ggagcgcttc atcggcattt tgcttgaaca tcacgccggc catctgccgc tctggctcgc
     4741 gccattgcag atcgtcgtct gcacgatcac gcaggacgcc gacgattacg cgatggagat
     4801 cgtcgccaag gcgcgcaagc gcggattgcg ggtcgaaggc gatctgcgca acgagaagat
     4861 cagctacaag gtgcgcgagc attcgctcgc caaggttccg gtcctgctcg tcgcaggcaa
     4921 gaaggaagcg ggcgaaagga cgatctcgat ccgccgtctc ggctcgaacg agcagaaggc
     4981 aatgagcctc gacgctgcgc tcgccgcctt ggtcgacgaa gccgttccgc cggaccttgc
     5041 gccggcgcgg cgcgcggcgg cggagtgacg gcgcgcatgg ccgccgttct cggcatcgac
     5101 gcagcctgga cggcgaagaa tgcgagcggc tatgcgctca tcggaaaaag cggcggcgcg
     5161 tggcgcctgc gcgccgccgc gccggacctt tgcgcgtttg cgcgaaaatg ccgtctggac
     5221 ggcgcggggc cggggatgga cggcgcgatc gcctgcgcta aaaaccttct cggccgcctg
     5281 cctgatctga tcgccgtgga catgccgctg tcgaaacagc agatcactgg ccgcagagcg
     5341 tcggatatcg gcgtgtcgcg gcgtttcggc gcggcgaaat gcgcgaccca ttcgccgtcg
     5401 gccgagcgtc ccggcaaggt gagcggcgcc tttcgcgccg cttgcgaaaa ggccggctac
     5461 gcactggtcg tctcggccgg cgccttgccc gctttgtcgc tcgccgaagt ctatcctcat
     5521 ccggccctgc tgcggttgat gcgcgcgggt gagcgcgtgc cgtacaagac cggaaagacc
     5581 aaaatctatt ggccgttggc cagttgcgac gagcggctgg cgcgcgtgac gcgctcgctc
     5641 ggcgcgatcg tcgatgcgct ggagagccac attgccggcg tcgcgcgcga gacggcgccg
     5701 ctcttcgccg ccgctgaaaa ttttgccggc ctgaagccgg ccgaagacat gatcgacgcg
     5761 atcgtttgcg cttggatcgg tacgaccatc ctcgccggcg aggccgagcc gatcggcgat
     5821 gcggattcgg cgatctggat tcccgacccg ctcaaaccga cgctgctggc aggcgccgca
     5881 tgatgcaagc ggcagcgcta tttcctcccc cgagaagcgg gggaggggga ccgcgcggag
     5941 cgcggtggag gaggttgcgg cgccggggat cattctcaac cgccgtaccc cctccaccat
     6001 gcttcgcatg gtccccctcc cccgcttcgc gggggaggat atgcgcggcg tacctctgat
     6061 gcgctgggtc gcctctttca agcccgacaa tttcaccctc gccattgcgg cggcggtcgt
     6121 catcgcaagc ttcttgccgg cgatcggctt ggcggcgacg gtcttgcatt acgtcacgat
     6181 cttcgccatc gcgctgttgt tcttcctgca tggcgcgcgg ctgtcgcgcg aggcggtgct
     6241 ggccggcgcc ttgcattggg ggctgcatct tatcgtgctc tgcgccacat tcgttctctt
     6301 tccggttctc ggccttatcc tgcgccgggc gcttggcccg gttctgccgg attctctgct
     6361 tgtcgggctg ctgtttctct gtcttctccc ctcgaccgtg cagtcctcga tcgccttcac
     6421 ctcgatcgct catggcaatg tcgcggcggc ggtatgcagc gcgtcgctgt ccaacctgct
     6481 cggcattttc atcacgccgg ctctcgtcgc gctgctgatg aacctgaaag gcggcgtgtc
     6541 gctgcatgcg gtgcttgaca tcgtcctgca actgctggcg ccgtttcttg tcggccattt
     6601 cctccggccg tggatcggcg ggttcgtcgc gcgccatcgc tatgcgctcg gctatgtcga
     6661 tcgcggctcg atcctcctcg tcgtctatct cgctttcagc gaggcggtcg ctaacgggct
     6721 ttggcacaag ctcggcgcgt ccgacatcgc caagttgctc atcgtctgcg ccgcattgct
     6781 ttgcgcggtg cttctcgcga cggtcacact cgcccgccgg ctcggctttt ccaggcccga
     6841 cgagatcgtc atcgtcttct gcggctcgaa aaagagcctc gccagcggcg tgccgatggc
     6901 cggcgtgctt ttcccgctgg cgcaagtcgg catgatcgtc ctgccgctga tgctcttcca
     6961 tcagattcag ttgatggtct gcgcggcctt ggccaagaga tacgccgcga gcgcggcgct
     7021 cgaaaccgtc cttgcggcga agccggaagc gggcggccgc gaagcggccg gatgacggcg
     7081 ccggcttccg cggtatgtac cgtcatggcg aggagcgaag cgacgaagca atccgtccta
     7141 gggctaagag gagaaaggcg ctacttcgga tcgagcggct cgacgccttt cggcttgccg
     7201 tcggcgccta aagtaatttt ctcgatgcgc atctcggcct ccctgagcag ccggtcgcaa
     7261 tgcgccttca aggcttcgcc gcgctcgtag attttgatcg attcttcgag cggggccgcg
     7321 cccttttcca gcttgtcgac gatggattcg agttcggcga gcgccgcttc gaagggcagc
     7381 ttggcgagat cgtcaggcgt gtcggtcacg agactgttcc ttgattttgc gcctgactat
     7441 cacaatcgcc ggcgccgcgc acgaggctca aatcggcagc tccgtcgtct ttttcaccgt
     7501 gcgcagcgcc aaggtcgatt gcacgcgcga gacgcccggc aactgcgtca ggatgtcggt
     7561 atggatgcgc tcgaaatcgg cggcgtcgcg ataggcgatg cgcagcaggt agtcggccga
     7621 gccggcgagc aggtagcatt cgatgatctc ggcgtggcgg cgcgcctcgg cttcgaaacg
     7681 gtcgagcgcg gcgcggcctt gctgatcgag cgtgacgaaa acgaaggcgg cgccgggcag
     7741 gccggcgcgg gccggatcga gcagcatcac atagccggcg atcaggccgc tctcctccag
     7801 cattcggacg cggcgcaggc aggcggaggg cgaaagattg acccgctcgg cgagatcggc
     7861 attggcgagc cggccctggc gcatcaaggc gcgaaggatt gcacggtcgc gctcatcaag
     7921 cggttcggat ggcatggtct tcggaggaaa ttgcgtctca aggacatttt atcgaattga
     7981 attcgaaaaa accagcgccg ctgctagaaa tatggcgaga aattgctcga aaaatggcgg
     8041 atcatgcgcc tctagctagg cgagggaaac cgtcatgctg atcggcgtgc cgaaggagat
     8101 caaggacaac gaattccgcg tcggcctgac gccgtcatcg gtggccgagc ttgtccatca
     8161 cggccaccag gtttgggtcg agcgatcggc tggaatcggc tcgggcctgc ccgattcgga
     8221 atatgcgcaa gcgggcgcga tattggtcga tggccaggac gacatcttcg cccgcgccga
     8281 gatgatcgtg aaggtcaagg agccgctcgc ggcggaacgc gagaagctgc ggcgcgggca
     8341 agtcttgttc acgtaccttc atctggcgcc cgatctggaa cagaccaagg acctgatcgc
     8401 ctccggcgcg acctgcatcg cttatgagac cgtcacctcg ccgcatggcg ggctgccgct
     8461 gctgacgccc at
//
LOCUS       DATGJW010000155         8819 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1166918, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000155 DATGJW010000000
VERSION     DATGJW010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8819)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8819)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8819
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1166918"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(274..1236)
                     /locus_tag="VKU03_04730"
     CDS             complement(274..1236)
                     /locus_tag="VKU03_04730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70598.1"
                     /translation="MILRLSATGRRALAALVIALALHGTGALLIDGYSSEFSIRSMLV
                     LATLLGVASIGQTLVILLGGIDLSTPFVIGFANVVAAQLYGEGHNFILVCLIVAAFAL
                     AIGAVNGALSSILSIHPLIVTLGIGTAVQGAVLLWTAGFPSGSAPQAVTDFVSIGGSF
                     GPLPVPGLIPFFVLLIAAVALMLERTPFGRRLYALGSNPQAAPYALIAPPAIWTATYA
                     LSALFAALAGVLLLGFTGSAYGDVGQPYLFQTIAAVVIGGTTLVGGRGGLIGTAAGAL
                     VLTEINTLLIGLGLSPAMVEAALGALIVGLVSIYGREQHVRTTI"
     gene            complement(1233..2222)
                     /locus_tag="VKU03_04735"
     CDS             complement(1233..2222)
                     /locus_tag="VKU03_04735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70599.1"
                     /translation="MSEVSKPARSIGARLTSLAAARRETGLALALFVLLAAVNIALNP
                     ARFAPTEWGTLIGLAAPLIAASASAMPAILGGRGGIDVSVGPLMGFINAMIVQTLLID
                     ANIASPWIIVPSALAIGVAAGALNGFLAVVVRIQPIVATLGTYLLFTGLTLTLVPAPI
                     GSAPAWIKSLSGTFSIAPLAGMAIVWLAIKQLPYYNQLMAVGSDDRACYTAGVPVSLV
                     RFFSYCATGLFAGVAGLLLTALIGSADPNVGPSFTLLAISSVALGGVSLGGGRGGLLA
                     ATVGAVDIFLLQSALTYFNVSTFVLQIAYGVILVASISLNALPAALGRRKAAL"
     gene            complement(2219..2980)
                     /locus_tag="VKU03_04740"
     CDS             complement(2219..2980)
                     /locus_tag="VKU03_04740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ70600.1"
                     /translation="MANDLQAAAERLRIEGVAVAAGAAPLDDSIRAGEIVGLAGLDGH
                     GQEAFLETLAGLRVAASGRVRVLVDGKPVAIASFRQAVRHGVAYLARDRRANGVFPSL
                     SILDNFGVVSMDRDCRFGLISRRAQRARYETYRQRLSIVAPAPDRPITALSGGNQQKV
                     LLARWLALEPRVLLLNDPTRGVDQRTRETLYIVFRELARAEGMALVVLSTEIEEVLRL
                     CDRVLVFREQRVAARLAGEALSNDRVIAAMFGRAA"
     gene            complement(2970..3710)
                     /locus_tag="VKU03_04745"
     CDS             complement(2970..3710)
                     /locus_tag="VKU03_04745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ70601.1"
                     /translation="MLDTDDLSKTYGETRALDGATIAFAPSTIHAILGENGSGKSTLV
                     KLLSGVVAPTRGAIRIDGRAATRFSPASFQEQGLATVFQEVLIAPDRSVVDNILLGRD
                     GLLTRATPRARRREVAREVLAPIAKTAIDLDAPAGALPLAARQLIVLARALVRRPRIL
                     ILDEATAALDFADRDAVFETMEAFARAGGLILFISHRMDEVMRLANRVTVLRNGRVVA
                     TRERAELSSRDMLRMMAPAAADEIAHGQ"
     gene            complement(3791..4999)
                     /locus_tag="VKU03_04750"
     CDS             complement(3791..4999)
                     /locus_tag="VKU03_04750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ70602.1"
                     /translation="MIRRANLRSRILGAAVALAGAVAIAGGAAKAANENLLEGLPDNL
                     KTLYEGETSTLQPSAYDNFKMPAPPWKWCHSESYQGNPWRVALTNEIKRLVDVYKKAG
                     KVSDWEFSDSNGDTSRQIAQIRAFIDKKCSIITAVAGSATGLNDAIEASYKAGIPFVT
                     ISSSVTSPYAINVDSNYSRWGYEMAEAIVKNLGGKGNVIMVEGIAGAPIVALERAGAD
                     KAFKGTDIKVVRSVNGNWTANVTKTVILQALATTPQKIDAVWTTGSESRVIAEAFAEA
                     KRPQPLITGSISGDALGYWKAHPDGYRFDGHAVLPGWNAQALFRVAVRLLEGQKPKLA
                     TLLIPIPAVHQDDLAKWYKSCMTPDSVSIFPSPPTDPVSNALLDAYFKDPKAIADYNY
                     ADVPDPCAGK"
     gene            complement(5152..6264)
                     /locus_tag="VKU03_04755"
     CDS             complement(5152..6264)
                     /locus_tag="VKU03_04755"
                     /EC_number="3.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF021778.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052689 - carboxylic ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactonase family protein"
                     /protein_id="HLJ70603.1"
                     /translation="MSAQIFAVIGSLTRDAPYFQGARGRGITVAAFDQATGRMTRLAE
                     KGGVDNPTFLAVHEGHRSVYASSEVFGWNEGVVSAYRLDPANGGLRYINMQPSLGSII
                     AHHSLDRTGRFLFVANYSVYAEPDDDQPNQAVIALPIRDDGGLGAPVSSQAHKGAGPN
                     AERQDRSHAHCVLPSPDNRFVVVADLGTDELVVYRFDAATGALSLGRAFKAAPGSGPR
                     HIAFHPSGRFLYAINELDSTVSAYAFDAAGSLAHLNTLSALPSAVSGESHGSGLQISP
                     DGQFLYGGVRGADILAVYAVDQSSGRIALVDLPSALGKTPRDLTIDPTGRFVVVCNQN
                     SDALAIFRIDRETGRLADTGHRFETGTPMCVKFLPA"
     gene            complement(6314..7348)
                     /locus_tag="VKU03_04760"
     CDS             complement(6314..7348)
                     /locus_tag="VKU03_04760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="succinylglutamate desuccinylase/aspartoacylase
                     family protein"
                     /protein_id="HLJ70604.1"
                     /translation="MVSDSATSRAKRERATTLTATVDFDKPGKQTGFIHVPFSSHDDA
                     WGVIPIPIAVISNGGGPTVLLEGGNHGDEYEGPISLGELIRDLDPGLVSGRLIIIPAI
                     NLPAVKAGLRTSPIDTLNFNRTFPGDPNGTPTQQISAYVNDVLFPMADAFLDLHSGGS
                     SLAMIPSAIIEPVDDPAHQERNAAAARAFGAPMIVAIDNRGDTRTATSAAARAGLTVV
                     GTEMAGGGTVSIDALALCRRGVRNVLAHLGALPPEMAAPSPTPSPVYTLPGALAYVLA
                     SDDGVFEPLHANGAEVRAGQPAGRIHNLANPARPPAELTYQSDGVLYGRRQPGRVRPG
                     NCCLVVARPV"
     gene            complement(7374..8324)
                     /locus_tag="VKU03_04765"
     CDS             complement(7374..8324)
                     /locus_tag="VKU03_04765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994502.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70605.1"
                     /translation="MTAALRRFAVVYARSLVAYGVVAALWIAASLTIAGFGAYAHLRY
                     IVELAAVIGLVGVGQTVAVIGGGIDLSVSAVITVTAIILPILSFDADTSGVGAIALSL
                     AVAAAIGAINGLGIGYLGLPPLIMTLAMATILQGLLVLIAGGSAISVNNAALDWLGSV
                     HVLTISASILLWLIVAAVCLFWLHGTRSGSLIFAIGTNPVVSRLSGAPIRRLTLATYV
                     ISGFCAGVAGLLLLSMNGQGYVGIGDPYLLSSIAAVVLGGTSILGGRGSYLGTIAGAI
                     LLVTMTALITVVNASEGWRSVILGCLILGMLPLSGRDQSR"
     gene            complement(8321..>8819)
                     /locus_tag="VKU03_04770"
     CDS             complement(8321..>8819)
                     /locus_tag="VKU03_04770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019994503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70606.1"
                     /translation="PSLSEFLAGGQPIAAVLLVLFAIFWKLYQATPLGVGLIAVGDNA
                     EGAYRSGVNVTAARLGAYILSGVLQALTGLYVAAQTGSGDPVIGEPLTLAAIAAAVLG
                     GVGFLGGQGTMRGALAGSLLMSILINVMFFMRLPLVAQYVVQGLIIVVTVALPLIRAS
                     WRRAT"
BASE COUNT         1620 a         2792 c         3093 g         1314 t
ORIGIN      
        1 cgtccgggtc caccacgaga acttgcccgt cgaggtcgag aagcacgtcg aaggtcgggt
       61 cacgcggctc cgtcacgcca tatagttatc acactgaagc aactgaagca agggagggct
      121 tgaggccgcg gtgggataga cgaatggcgc tcgacgcatt tgctctcatt gagctttcgg
      181 ccatgacgac cagcagagcg tattatgaat agcttcaata aggccgaggc gcgccgcctc
      241 actgggctat agtgtagacg ccgcgccatg ccctcaaatc gtcgtccgca cgtgctgctc
      301 gcgtccatag atggagacga gcccgacgat gagcgctccg agcgccgcct cgaccatggc
      361 cggcgagagg ccgaggccga tcaggagcgt gttgatctcg gtcaggacca gcgcgccggc
      421 ggcggttccg atgaggccgc cgcgtccgcc gaccagggtc gttccgccga tcaccacggc
      481 ggcgatggtc tggaaaagat agggctgccc gacatccccg taggccgagc cggtgaagcc
      541 gagcagcagc acgccggcga gcgccgcaaa caaggcgctg agggcgtagg tcgccgtcca
      601 gatcgcgggc ggcgcgatga gggcgtaagg tgcggcttgc ggattgctgc cgagggcata
      661 gaggcgccgc ccgaagggcg tgcgctcgag catgagcgcg acggcggcga tgagcaggac
      721 gaagaacggg atcaagccgg ggaccggcag agggccgaaa ctgccgccga tcgaaacgaa
      781 atcggtgacc gcttgcggcg cggagccgga cggaaatccg gccgtccaca gaagcaccgc
      841 gccctggacg gcggtgccga ttccgagcgt gacgatgagc ggatggatgg acaggatcga
      901 cgacagggcg ccgttgaccg cgccgatggc gagcgcgaag gcggccacga tcaggcaaac
      961 caggatgaaa ttgtggccct cgccgtaaag ctgcgcggcg acgacattgg cgaagccgat
     1021 gacgaagggc gtcgagaggt cgatgccgcc gagcaggatg accagcgtct ggccgatgga
     1081 ggcgacgccg agcagggtcg ccaggacgag catcgagcga atcgagaact cgctgctata
     1141 gccgtcgatc agcagcgcgc cggtcccgtg cagggcgagg gcgatgacga gcgcggcgag
     1201 cgcccgccgg ccggtggcgc taaggcgcag gatcatagcg ccgcctttcg ccggccaagc
     1261 gccgccggca gcgcgttgag ggaaatcgag gcgaccagga tgacgccata ggcgatttgc
     1321 agcacgaagg tcgagacgtt gaaataagtc agcgcgcttt gcagcaggaa gatatcgacc
     1381 gcgccgacgg tcgccgccag caggccgccg cgcccgccgc caaggctgac gccgccaagc
     1441 gcgacggacg agatcgcgag cagggtgaag ctcggcccga cattcggatc ggccgagccg
     1501 atcagcgccg tcagcaacag gccggctacg cccgcgaaca gcccggtggc gcaataggaa
     1561 aaaaagcgca ccagcgagac cggcacgccg gcggtgtagc aggcgcggtc gtcgctgccg
     1621 accgccatca attggttata gtacggaagc tgcttgatcg cgagccagac gatcgccatg
     1681 ccggcgagcg gcgcgatcga aaacgtgccc gataggctct tgatccatgc cggcgccgag
     1741 ccgatcggcg cgggcacgag cgtcagcgtc aggccggtga aaagcaggta ggtcccgagc
     1801 gtcgcgacga ttggctggat gcgcacgacg acggcgagga agccgttgag cgcgcccgcg
     1861 gcgacgccga tcgccaaagc ggacgggacg atgatccaag gcgaggcgat attcgcatcg
     1921 atcagcagag tctgcacgat catcgcattg atgaagccca tcagcgggcc gaccgacacg
     1981 tcgatcccgc cgcgtccgcc gagaatcgcc ggcatggcgg aggcggaggc cgcgatcagc
     2041 ggcgccgcca ggccgatcag cgtgccccat tcggttggcg cgaagcgggc gggattgagc
     2101 gcgatgttga cggcggcgag cagtacgaag agggcaagcg ccaagccggt ctcgcgccgc
     2161 gccgcggcga ggctcgttag ccgcgcgccg atcgaccgcg ccggctttga gacctcgctc
     2221 acgccgcccg cccgaacatg gccgcgatca cccggtcgtt gctgagggcc tcgccggcaa
     2281 ggcgcgccgc gacgcgctgc tcgcggaaca cgagcacgcg gtcgcagagc cgcaagacct
     2341 cctcgatctc tgtcgacagg acgacaagcg ccatgccctc agcgcgcgca agctcgcgga
     2401 aaacgatata gagcgtttcg cgggtgcgct ggtcgacgcc gcgcgtcgga tcgttgagca
     2461 gaagaacgcg cggctccaga gccagccagc gcgccagcaa gactttctgc tggttcccgc
     2521 cggaaagcgc ggtgatcggc cggtcgggcg cgggcgccac gatggagagg cgctggcgat
     2581 aggtctcata gcgggcgcgc tgcgccctcc ggctgatcag cccgaagcgg caatcccggt
     2641 ccatcgagac aacgccgaaa ttgtcgagga tcgacagcga gggaaagacg ccgttggcgc
     2701 gccggtcgcg cgccagatag gcgacgccgt ggcgcaccgc ctggcggaag ctcgcgatcg
     2761 ccaccggctt gccgtcgaca aggacccgga cacgtccgga ggcggcgacg cggaggccgg
     2821 cgagcgtttc gagaaaggcc tcttggccat gcccgtcgag gccggccagg ccgacgattt
     2881 ctccggcgcg gatgctgtcg tcgagcggcg cggcgccggc cgcgacggcg acgccttcga
     2941 tgcgcaagcg ctcggccgcc gcttgaagat cattggccat gggcgatctc gtcggccgcc
     3001 gcaggagcca tcatccgcag catgtcgcgt gaggaaagct cggcgcgctc gcgggtcgcg
     3061 acgacccggc cattgcgcag gaccgtgact cgattcgcga ggcgcatgac ctcgtccatg
     3121 cggtgcgaaa tgaaaaggat gagcccgccg gcgcgcgcga aagcctccat tgtttcaaac
     3181 acggcgtcgc ggtcggcgaa gtcgagcgcc gctgtcgcct cgtcgagaat gaggatgcgc
     3241 ggccggcgca cgagagcccg agcgagaacg ataagctgtc gcgccgcaag cggcagcgcg
     3301 ccggcgggcg cgtcgaggtc gatcgccgtc ttggcgatcg gcgcaagcac ctctcgcgca
     3361 acctcccgcc gcctggcgcg cggcgtcgcg cgggtcagaa gcccgtcgcg gccgagcagg
     3421 atattgtcga cgacgctgcg gtcgggcgcg atcagcactt cctgaaagac ggtcgcaaga
     3481 ccctgttcct ggaaagaagc gggggaaaac cgcgtcgccg cgcgcccgtc aatgcggatc
     3541 gcccctcgcg tcggcgcaac gacgccggaa agcagcttga ccagcgtgct cttgccggag
     3601 ccattctcgc cgagaatggc gtgaatggtc gagggcgcga aggcgatcgt tgcgccgtca
     3661 agcgcccgcg tctcgccata ggttttgctc agatcgtcag tgtcgagcat cgcggcagat
     3721 caaacaaaga cagccgcccg gagagccggg cgactgtcgt accgataagc gcgcccctat
     3781 cgggagcgcg tcatttgccg gcgcacggat cggggacgtc ggcgtagttg taatcggcga
     3841 tcgccttggg atccttgaaa taggcgtcga ggagcgcgtt gctgaccggg tcggtcggcg
     3901 gcgagggaaa gatcgaaacc gaatccggcg tcatgcagct cttgtaccat ttggcgaggt
     3961 cgtcctggtg gacggccggg atggggatca ggagcgtggc gagcttcggc ttctgaccct
     4021 caagaaggcg aacggcgacg cggaacaggg cctgcgcgtt ccagccgggc agaacggcgt
     4081 ggccgtcgaa ccggtagccg tccggatgcg ccttccagta gccgagcgcg tcgcccgaaa
     4141 tcgagccggt gatcaacggc tgcggccgct tggcttcggc gaaagcctcg gcgatgacgc
     4201 gcgactcgct gcccgtcgtc cagaccgcgt cgattttctg cggggtggtg gccagagcct
     4261 gcaagatcac ggtcttggtg acattggcgg tccaattgcc gttgaccgag cgcacgacct
     4321 tgatgtcggt acccttgaag gctttgtcgg cgccggcccg ctcgagcgcg acgatcggcg
     4381 cgccggcaat gccctcgacc atgatgacat tgcctttgcc gccgaggttc ttcacgatcg
     4441 cctcggccat ctcgtagccc cagcgcgaat agttcgagtc gacattgatc gcgtaggggc
     4501 tggtcaccga gctcgatatc gtaacgaacg gaattcccgc tttgtacgac gcctcgatcg
     4561 cgtcgttgag gccggtcgcc gagcccgcta cggcggtgat gatcgagcat ttcttgtcga
     4621 taaaggcgcg gatctgcgcg atctggcggc tggtgtcgcc gttcgagtcg gagaactccc
     4681 aatcgctgac cttgcccgcc ttcttgtaga cgtcgaccag gcgcttgatc tcattggtca
     4741 gcgcgacgcg ccatggattg ccctggtagg attccgaatg gcaccatttc cacggcggcg
     4801 ccggcatttt gaaattgtca taggccgaag gctgcagcgt gctggtctcg ccttcgtaca
     4861 gcgtcttgag attgtccggc agcccttcca acaggttctc gttcgcggct ttcgccgcgc
     4921 cgccggcgat cgcgacggcg ccggccaggg cgaccgccgc tcctagaatt cgcgatcgca
     4981 aatttgctcg tctgatcatg gcttcctccc ttacttgccg gcgcccggcc gcggccgagt
     5041 ccgccttccg ccgtttcgca tcagcgatgc ggatcggcgc tgccgcatgg cgccatcata
     5101 gcgaacgcgc ttcgcatatg aaagagtcat ttatacccaa atgactaccc ttcacgccgg
     5161 caagaacttc acgcacatcg gggtccccgt ctcgaaccga tggcctgtat ccgccaaccg
     5221 cccggtctct ctgtcgatcc ggaagatggc gagggcgtcg ctgttctgat tgcagaccac
     5281 gacgaagcgc cccgtcgggt cgatcgtcag atcgcgtggc gttttgccga gcgccgacgg
     5341 cagatcgacc aaagcgatgc ggcctgaact ctggtcgacc gcatagaccg ccaggatgtc
     5401 ggcgccgcgc accccgccat aaaggaattg gccgtccgga ctgatctgca agccggatcc
     5461 gtgactctcg ccgctgacgg cggaggggag cgctgagagc gtgttgaggt gggcgagaga
     5521 gcctgcggcg tcgaaagcgt aggcggaaac cgtcgagtca agttcgttaa tggcgtagag
     5581 gaaacggccg gaggggtgaa acgcgatgtg gcgcggtccc gaaccgggcg cggccttgaa
     5641 ggcacggccc aagctcagcg cgcccgtcgc cgcgtcgaac cgataaacga cgagttcgtc
     5701 ggtcccgaga tcggcgacga cgacaaagcg gttgtcgggg ctcggcagaa cgcaatgggc
     5761 gtgggagcgg tcctggcgct cggcgttggg gccggcgccc ttatgggcct ggctgctgac
     5821 cggcgcgccg aggccgccat cgtcgcggat tggcagcgca atgaccgcct ggttgggctg
     5881 atcgtcgtcg ggctcggcat agaccgaata attcgcgaca aaaaggaagc gtccggtccg
     5941 atccaaactg tgatgcgcga tgatgctgcc gagggagggc tgcatattga tgtagcgaag
     6001 cccgccattg gccggatcga gccgataggc ggagacgacg ccttcgttcc agccgaacac
     6061 ctcgctgctg gcgtagacgc tccgatggcc ctcgtggacg gcgagaaagg tcggattgtc
     6121 gacgccgcct ttttcggcga ggcgcgtcat ccggccggtc gcttggtcga aagcggcgac
     6181 ggtgattccc cgtccgcgcg cgccttgaaa gtagggcgcg tcgcgggtca ggctgccgat
     6241 cactgcgaaa atctgggcgc tcatggtcct tctcgcttcg ctctttcctg gtgtatccgg
     6301 cgtcggcggt tctctaaacc ggccgcgcaa cgaccaggca gcaattgccg ggtctgactc
     6361 ggccgggctg acgccgccca tagagcacgc cgtcggattg gtacgtcagc tcggctggcg
     6421 gccgggcggg attggcgagg ttgtgaatgc ggcccgccgg ctggccggcg cgcacttcgg
     6481 cgccgttggc gtggaggggc tcgaacacgc cgtcgtcgct cgcgagcacg taagcgagcg
     6541 cgccgggcag ggtatagacc ggcgatggcg tcggcgaggg ggcggccatt tcgggcggca
     6601 gcgcgccgag atgggcaagg acattgcgca cgccgcgccg gcaaagcgcg agggcgtcga
     6661 tcgacaccgt tccgccgccg gccatctcgg ttccgacgac ggtcaatccg gcgcgcgccg
     6721 ccgccgaggt cgcagtgcgc gtgtcgcccc ggttgtcgat cgcaacgatc atcggcgcgc
     6781 cgaaggcgcg cgccgccgcg gcgttgcgct cctgatgggc cggatcgtcg accggctcga
     6841 tgatcgcgct cgggatcatg gcgagcgacg acccgcccga atggagatcg aggaaggcgt
     6901 ccgccatcgg aaacaggacg tcgttgacat aggcgctgat ttgttgcgtc ggcgtcccgt
     6961 tgggatcgcc gggaaaggtg cggttgaaat tgagcgtgtc gatcggcgac gttctcagtc
     7021 ccgctttgac ggccggcagg ttgatcgccg gaatgatgat cagccggccg gagacgagcc
     7081 cgggatcgag atcgcggatg agttcgccga ggctgatcgg accttcatac tcgtcgccgt
     7141 gattgccgcc ttcgagcagg actgtcggcc ctccgccgtt gctgatgacc gcgatgggaa
     7201 tgggaatgac gccccaggcg tcgtcatgcg acgagaaggg gacatggatg aagcctgtct
     7261 gcttgccggg cttgtcgaaa tcgacggtcg ccgtgagcgt cgtcgcgcgc tcgcgcttcg
     7321 cccttgatgt cgccgaatct gagaccatga gcatttcctc gtcgaaaccc gcttcagcgc
     7381 gactggtcgc gccccgatag cggcagcatg ccgagaatga ggcagcccag aatgacgctg
     7441 cgccaaccct ccgacgcgtt caccacggtg atgagcgcgg tcatcgtcac cagcaggatc
     7501 gccccggcga tcgtgccgag atagctgccg cgcccgccga gaatcgacgt gccgccgagc
     7561 acgaccgccg cgatcgacga caacaggtac ggatcgccga tgccgacata gccttggccg
     7621 ttcatcgaca agagcaggag cccggcgact cctgcgcaga atccagaaat gacataggtt
     7681 gccagcgtca gccgccggat cggcgcgccc gacagcctgc tgacgactgg gttcgtgccg
     7741 atcgcgaaga tcagcgagcc gctgcgcgtt ccatgcagcc agaagagaca caccgcggcc
     7801 acgatcagcc acaggagaat gctggcggag atcgtcagca catgtacgga gccgagccag
     7861 tccaacgcgg cgttgttgac cgagatcgcg ctgcctccgg cgatcaacac gagcagacct
     7921 tgcaggattg tcgccatggc gagcgtcatg atgagcggcg gaagcccgag atagccgatc
     7981 cccaagccat tgatcgcgcc gatggcggcg gcgacggcga gggaaagcgc gatggcgccg
     8041 acgccggacg tatcggcgtc gaacgacagg atcggcagaa tgatcgccgt cacggtgatg
     8101 accgcgctga ccgaaaggtc gataccgccg ccgatcaccg cgacggtctg gccgacgccg
     8161 acaagcccga tcaccgccgc gagctcgacg atgtagcgca gatgcgcgta ggcgccgaag
     8221 ccggcgatcg tcagcgaggc ggcgatccac agcgcggcga cgacgccgta ggcgacgagc
     8281 gagcgggcgt agacgacggc gaagcgccgc aaggcggcgg tcatgtcgcc cgccgccagg
     8341 aagcgcggat caggggcagc gcaacggtca cgacgatgat cagaccctga acgacatatt
     8401 gcgcgacgag aggcagtcgc atgaagaaca tgacgttgat cagaatgctc atcagcaagc
     8461 tgccggcgag cgcgccccgc atggtcccct ggccgccgag gaagccgacg ccgccaagga
     8521 ccgcggcggc gatcgcggcg agcgtcaggg gctcgccgat gaccgggtcg cccgagccgg
     8581 tttgcgcggc gacgtagagg ccggtcaaag cctggaggac gccgctgagg atataggcgc
     8641 cgaggcgcgc cgccgtgacg ttcacgccgg aacggtaggc gccctcggca ttgtcgccga
     8701 cggcgatcag gccaacgccg agcggcgtcg cctgatagag cttccagaag atcgcgaaca
     8761 ggacgagcag gaccgccgca atcggctggc cgccggcgag gaactccgac agcgaggga
//
LOCUS       DATGJW010000156         4978 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1479371, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000156 DATGJW010000000
VERSION     DATGJW010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4978)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4978)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4978
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1479371"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..521)
                     /locus_tag="VKU03_04775"
     CDS             complement(<1..521)
                     /locus_tag="VKU03_04775"
                     /EC_number="1.15.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011440573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004784 - superoxide dismutase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="superoxide dismutase"
                     /protein_id="HLJ70607.1"
                     /translation="MSFTLPDLPYPHDALAPYMSKETLEFHHDKHHLAYVNNGNNLLK
                     GTEWEGKSLEEVVKGSYGKNAGLFNNAGQHYNHIHFWKWMKPKGGGKIPGELEKAIAS
                     SLGSVDKAKEEFIQAGATQFGSGWAWLAVKDGKIAVMKTPNGENPLVHGARPILGVDV
                     WEHSYYIDYRNRRP"
     gene            complement(707..2509)
                     /locus_tag="VKU03_04780"
     CDS             complement(707..2509)
                     /locus_tag="VKU03_04780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HLJ70608.1"
                     /translation="MHEAPNFALYRETLLFLATAGIVAPIFIRLRISPVLGYLIAGAA
                     LGPHGLGLLVGRFSWLAPFALTNVEQIDTIAALAVVFLLFMMGLGLSFERLSRMRRLV
                     FGLGLAQVVLCSVLLGAAALALGQSLPAAAVIGAALSLSSTSIVIPVLAERKRLNSAA
                     GRASFSVLLFQDLAVAPLLFMTTMLARGKTDGFGADLLWTLAPAALALVGLIVLGRLV
                     LRPLFHSVALTKSPELFMAVCLLVVIGAGLAAAWSGLSMALGAFIAGLLLAETEFRRQ
                     IEVTIEPFQGLLLGLFFVSVGAGLDLSLFAASPLRIVAIAAGLIAIKAAAVLGLGRAF
                     KAPARVAREVALLLGPGGEFAFVMLGAAIASGVVAAEAGRSALVAVTLTMLLIPLLGR
                     LGERVAQSKTASGAAAEAFEVPPEGGGAARVIIIGFGRVGALVGDMLARHQISFIAVD
                     FDAKLVAKARAEGAPIYFGDATRPDFLRRCGIETARALVVTMDAPRANEAVVETARAL
                     RADLTIVARARDAEHAKTLYDFGVTDAVPETIEASLQLSEAVLVDIGVPMGYVIASIH
                     DKRDEFRKALIASGAPARPRMAKRTRVGTADAPR"
     gene            complement(2551..3858)
                     /locus_tag="VKU03_04785"
     CDS             complement(2551..3858)
                     /locus_tag="VKU03_04785"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLJ70609.1"
                     /translation="MVDSALRKRIVIVGAGFGGMAAAKGLKGVDAEITLVDRTNHHLF
                     QPLLYQVATAALSPADIAAASRALLRRQRNISVLMAEVTGIDPDRRLVHLAGGRDLPY
                     DFLILAVGAAYSFFGHESWRPFAPTLKSLADALAIRVRLLGAFEKAEECGDPALIKKL
                     MTFVVVGAGPTGVELAGTIAELARSTLARDFKAIDPRSARVILCEAGDRALSAFPRRL
                     SDYAAKALRGLGVELRFGKAVKEIDADGLIVGETRIEAANVLWCAGTEARPAARWLGA
                     DAARNGAVKVQADCSLPGRPEIFVIGDAASCDDGAGKPLPGLAPVAKQQGEYVAKTIK
                     ARLAGKPPPGPFRYRNYGTMAIIGRSRAIADFGAFTLTGFPAWLTWSLVHLMLLVDFR
                     SRLAVYLNWSWAWFTHGRGARLLTGEAGSRDLMRDIDRVREST"
     gene            complement(3924..>4978)
                     /locus_tag="VKU03_04790"
     CDS             complement(3924..>4978)
                     /locus_tag="VKU03_04790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multidrug effflux MFS transporter"
                     /protein_id="HLJ70610.1"
                     /translation="YFLAFGLCQVFYGPISDMIGRKPPLYIGLALFVVGSVGCAFAPN
                     ISWLIAFRFVQGAGTACTMAIPRAIIRDLHTGLEATRLMSTIMLVISVSPILAPLIGS
                     GLIVPFGWRAVFVAVTIAAFVSFALLLLFLPETRPAEARESISLGGLARGFAQLLSDW
                     RFLGLTFIGGFGMASFFVFLAMSSFVYMGHFGLTPTQYSALFAANAVGFIGSSQFAAK
                     LGGRFGMARVVMGAVSLYAAFALFLLAVTATGVDSLAVLVVLLFCAFACLGLVIPPTM
                     VLALEEHGPLAGLASALGGTLQMAAGGVTIALASLVFNGTALPMAATIALCSVGALIL
                     AVATLGRRELAPQAAE"
BASE COUNT          982 a         1609 c         1715 g          672 t
ORIGIN      
        1 ggccggcgat tgcggtagtc gatatagtag gagtgctccc agacatcgac gccgagaatc
       61 ggacgggcgc cgtggacgag cgggttctcg ccgttaggcg tcttcatgac ggcgatcttg
      121 ccgtccttga cggcgagcca cgcccagccg gagccgaact gagtggcgcc ggcctggatg
      181 aactcttcct tggccttgtc gaccgagccg agcgacgagg cgatcgcctt ctccaattcg
      241 ccgggaatct tgccgccgcc tttcggcttc atccatttcc agaaatggat gtggttgtag
      301 tgctggccgg cattgttgaa gaggccggcg tttttgccgt acgagccttt gacgacctct
      361 tcgagcgact tgccctccca ttcggtgccc ttgagcaggt tgttgccgtt gttgacatag
      421 gcgaggtggt gcttgtcgtg gtgaaattcg agcgtttcct tcgacatgta cggcgcaagc
      481 gcgtcatgag gataaggcag atcgggcaat gtgaaggaca tgagcaaact ccatcaatcg
      541 aagaggaacg aggagatcgg cggcgaaagg ccgcgtcggc gccggcattg ttaaacccgc
      601 ggggcggcgc tcgcaaccga gaccggcttg cgccgctcac gcgatcgtga aaccgcaggg
      661 cgaagcgaag gttgcgcatt ttccggtcaa aaattcgaca gcgccgtcag cgcggcgcat
      721 cggccgtccc gacgcgggtg cgcttggcca tgcgggggcg cgccggcgcg ccggaggcga
      781 tcaacgcctt gcgaaattcg tcgcgtttgt cgtggatcga ggcgatgacg tagcccatcg
      841 gcacgccgat atcgacaaga accgcctcgg agagttgcag gcttgcttcg atcgtttccg
      901 gcaccgcgtc ggtgacgccg aaatcgtaga gcgttttggc gtgctcggcg tcgcgggcgc
      961 gcgccacgat ggtgaggtcg gcgcgcagcg cccgcgccgt ctcgaccacc gcctcgttgg
     1021 cgcgcggcgc atccatcgtc accaccaagg cgcgcgccgt ctcgatgccg cagcgcctca
     1081 agaaatccgg ccgcgtcgcg tcgccgaaat agatcggcgc gccctcggcg cgcgctttgg
     1141 ccaccagctt ggcgtcgaaa tcgacggcga tgaaagaaat ttggtgacgc gcgagcatgt
     1201 cgccgaccaa tgcgccgacc cggccaaagc cgatgatgat gacccgcgcc gcgccgccgc
     1261 cttcgggcgg gacctcgaag gcctcggccg cggcgccgga agcggtcttg ctttgcgcga
     1321 cgcgctcgcc gagccggccg aggagcggga tcagcaacat cgtcagggtg acggcgacca
     1381 aggcggagcg cccggcctcc gcggcgacca cgccgctggc gatcgccgcg ccgagcatca
     1441 cgaacgcgaa ttcgccgccg ggaccgagca acagcgcgac ctcgcgcgcg acccgggccg
     1501 gcgccttgaa cgcccggccc aaaccgagca ccgccgccgc cttgatcgcg atcagtccag
     1561 ccgcgatggc gacgatccgg agcggcgaag cggcgaaaag cgagagatcg agcccggcgc
     1621 cgaccgagac gaagaacagg ccgagcaaga gaccctggaa cggctcgatc gtcacctcga
     1681 tttgccgccg gaactcggtc tcggcgagaa gcaggccggc gatgaaagcg ccgagcgcca
     1741 ttgagaggcc gctccacgcc gcggccaagc ccgcgccgat cacgaccaaa aggcaaaccg
     1801 ccatgaagag ttcggggctc ttggtcaggg cgacggagtg gaacaagggc cgcaggacga
     1861 gccgcccgag cacgataagg ccgaccagag ccagcgccgc cggcgccagc gtccaaagca
     1921 gatcggcgcc gaatccgtcg gttttgccgc gggccaacat cgtcgtcatg aacaacagcg
     1981 gcgcgaccgc gagatcttga aacaggagga cggagaagct cgcccgtccg gccgcggaat
     2041 tgagccgctt gcgctcggcg aggaccggaa tgacgatcga ggtcgaggat agcgacagag
     2101 ccgcgccgat caccgccgcg gcgggcagag attggccgag cgccaaggcc gccgcgccga
     2161 gcaggaccga gcacaggacg acctgggcaa ggccgaggcc gaaaaccagg cggcgcatgc
     2221 gcgacaggcg ctcgaaggaa aggccgagcc ccatcatgaa gagcaggaac acgaccgcga
     2281 gcgcggcgat cgtatcgatc tgctcgacat tggtcagcgc gaacggcgcg agccaggaaa
     2341 accgtccgac gagcagcccg agcccgtgcg gaccgagcgc cgcgccggcg atcagatagc
     2401 cgaggaccgg cgatatgcgc agccggatga agatcggcgc gacgattccg gcggtcgcca
     2461 ggaacagcag cgtttccctg taaagcgcga aattgggggc ttcgtgcaac cgctgctccg
     2521 ctcgagtgat gctcggtcct ggcgatcaag ctaggtcgat tctcgaacgc ggtcgatatc
     2581 gcgcatcaaa tcgcgcgagc ccgcttcgcc ggtcagcagc cgcgcgccgc ggccatgggt
     2641 gaaccaggcc caggaccagt tcaggtagac ggcgagccgg ctgcgaaaat cgaccaacag
     2701 catcagatgg accagcgacc aagtgagcca cgcgggaaag ccggtcaagg tgaaggcgcc
     2761 gaaatcggcg atggcgcgcg agcggccgat gatcgccatc gtaccataat tgcgataacg
     2821 gaacgggccg ggcgggggct tgccggcgag acgcgccttg atcgtcttgg cgacatattc
     2881 gccctgctgc ttggcgaccg gggcgaggcc cggcagcggc ttgcctgctc cgtcgtcgca
     2941 gctcgcggcg tcgccgatca cgaaaatttc cggccgtccg ggcaaagagc aatcggcctg
     3001 caccttgacc gcgccgttgc gcgccgcatc ggcgccgagc cagcgcgccg ccggacgcgc
     3061 ctcggttccc gcgcaccaca agacgttggc ggcctcgatt cgcgtttcgc cgacgatcag
     3121 cccgtcggcg tcgatctcct tgaccgcttt gccgaagcgc agctcgacgc cgagcccgcg
     3181 cagcgccttc gccgcataat cggagagacg gcgtgggaac gccgacagcg cgcgatcgcc
     3241 tgcttcgcag aggatcaccc gcgccgaacg cggatcgatc gccttgaagt cgcgcgccaa
     3301 ggtcgagcgg gcgagctcgg cgatcgtgcc ggcgagctcg acgccggtcg ggccggcgcc
     3361 gaccacgacg aaggtcatga gctttttgat cagggccgga tcgccgcatt cctcggcttt
     3421 ctcgaaagcg ccgagcagac gaacgcgaat cgccagcgcg tcggcgagcg acttcaaggt
     3481 cggcgcaaaa ggccgccagg attcgtggcc gaagaaacta taggccgcgc cgaccgccag
     3541 gatgagaaaa tcgtagggca aatcgcggcc gccggcgaga tgaacgagcc ggcggtccgg
     3601 atcgatcccg gtcacttcgg ccatcagcac gctgatgttg cgctggcggc gcagcagcgc
     3661 ccggctcgcc gcggcgatgt cggccggcga caaagccgcg gtcgccactt gatagagcag
     3721 cggctggaac agatgatgat tggtacgatc gaccagcgtg atctcggcgt cgacgccttt
     3781 caaccccttg gccgccgcca tgccgccaaa gccggcgccg acgatgacga tgcgcttgcg
     3841 aagggctgaa tcgaccataa ggcgctctgc tccgagcaga tttgtagccg cggtcggcga
     3901 ccgcggctac agcacgccgc gcgctactcc gccgcttgcg gcgccaattc gcgacggccg
     3961 agcgtcgcga cggcgaggat cagcgcgccg actgagcaca gggcgatcgt cgccgccatc
     4021 ggcagcgccg ttccgttgaa gacgaggctc gccagagcga tcgtcacgcc gccggccgcc
     4081 atttgcagcg tgccgccaag cgccgaggcg aggccggcga gggggccatg ctcttcgagc
     4141 gcgaggacca tggtcggcgg aatgaccagg ccgagacagg cgaaagcgca gaacaaaaga
     4201 acgacgagga cggcgaggct atccaccccc gtagccgtca cggccaggag aaacagggcg
     4261 aaggccgcgt agagagagac cgcccccatc accacgcgcg ccatgccgaa ccggccgcca
     4321 agcttggcgg cgaattgcga cgagccgatg aagccgaccg cattggcggc gaaaagcgca
     4381 ctgtattgcg tcggggtcag gccgaaatgg cccatataga cgaaggacga catggcgagg
     4441 aacacgaaga agctcgccat gccgaagccg ccgatgaacg tgaggcccag gaagcgccaa
     4501 tcgctcagaa gttgcgcgaa gcctctggcg aggccgccga gcgagatcga ctcgcgcgct
     4561 tcggccgggc gggtttcggg gaggaacaac agaagcagcg cgaagctgac gaaggcggcg
     4621 atcgtcaccg cgacgaaaac ggcgcgccag ccgaacggga cgatcaagcc gctgccgatc
     4681 aacggcgcca ggatcggcga aacgctgatg accagcatga tcgtcgacat cagccgcgtc
     4741 gcttcgagcc cggtatgcag gtcgcggatg atggcgcgcg ggatcgccat cgtgcaggcg
     4801 gttcccgcgc cctgcacgaa gcggaaggcg atcagccacg agatattcgg cgcgaaggcg
     4861 cagccgaccg agccgacgac gaagagggcg aggccgatat agagcggcgg cttgcgtccg
     4921 atcatgtcgg agatcggtcc gtagaaaacc tggcagaggc cgaaggcgag gaagtata
//
LOCUS       DATGJW010000157        14294 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_754426, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000157 DATGJW010000000
VERSION     DATGJW010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 14294)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14294)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14294
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754426"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2326
                     /gene="mutS"
                     /locus_tag="VKU03_04795"
     CDS             <1..2326
                     /gene="mutS"
                     /locus_tag="VKU03_04795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLJ70611.1"
                     /translation="GTITEERLLEPGRANLLVAIHRLRAGEDRTLFGVAAVDISTGAF
                     WLSESDAASLSAEIARLEPSEIIATQATLDDPDAARAFAETRAAVTPIGREASTGEAA
                     EQRLREFYGVETLDGFGAFSRAEIGAAALAIHYVGRTQLEARPALERPSRRPRGETME
                     IDAATRANLELTRTLSGQRQGSLLATIDLTATPAGARLLAERLAGPLTDPATINRRLD
                     AVGYFIEEQRLRGDLRARLRAAPDIARAMSRLSLDRAGPRDLAALRDGLGVARLVAAL
                     LAAQPSGSVIIAAAARRLAQMAPVLEQALSETLSDNLPLNKRDGGFVREGAEPALDEA
                     RALRDESRRVIAELQGKYCSLAETTQIRIKHNHFLGFFIETPQAHGERLLKAPYAATF
                     IHRQTMAGAMRFSTNELAELEAKIASAADRALALELAAFERLRQASLAELDAIRGLGE
                     ALAEIDVAAALAELAASRDWVRPVVDDSLTFVVEAGRHPVVEAALQAQGEPFVANDCT
                     LSGEIGEGGCIALVTGPNMAGKSTYLRQGALIALLAQAGSYAPARRVHIGIVDRLFSR
                     VGAADDLARGRSTFMVEMVETAAILNQATQRSLVILDEIGRGTATYDGLSIAFATIEH
                     LNAVNRSRALFATHYHELTRLAATLPRMTNLALRVTEWKGDLVFLHEVAPGAADRSYG
                     VQVAKLAGLPSAVVERARTILAELESGNRSGSALKKLDDLPLFSYAPKPPEAAPKRDA
                     LRDKLATIDPDEMTPREAQAALYGLKQLAKEDEG"
     gene            complement(2483..2866)
                     /locus_tag="VKU03_04800"
     CDS             complement(2483..2866)
                     /locus_tag="VKU03_04800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70612.1"
                     /translation="MIKALVSLGFAGALVVAPISLSRAHEIYTGVHGRNGQLCCGGDD
                     CAATVYREHGGTFEFLTRENEWVAIPEERITFLPIPGDPPSDNMHHAHLCYRQATAYD
                     RQGQAATNVFEHIFLYCAFIPPGSI"
     gene            complement(3358..4008)
                     /locus_tag="VKU03_04805"
     CDS             complement(3358..4008)
                     /locus_tag="VKU03_04805"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLJ70613.1"
                     /translation="MPLVTLSFDNGPHPGVTPMALQTLARYAIPATFFVLGKNIEAPE
                     GRAAMAEAHARGHWIGNHTYHHETPLGLIEDPAASVEEIERTDALIGDYASPSPLFRP
                     YGQGGMLDRRLLSEAAVDHLLKRRATCVTWNVVPRDWSDPYGWVDTALRQIGEQEESL
                     VVLHDIPTGAMVHLERFIEGALALGAAFRQDFPDDCVLIREGSPTAALSAYVTRRQ"
     gene            complement(4121..6406)
                     /locus_tag="VKU03_04810"
     CDS             complement(4121..6406)
                     /locus_tag="VKU03_04810"
                     /EC_number="1.1.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921651.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004470 - malic enzyme activity [Evidence
                     IEA];
                     GO_function: GO:0004471 - malate dehydrogenase
                     (decarboxylating) (NAD+) activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent malic enzyme"
                     /protein_id="HLJ70614.1"
                     /translation="MTKETEGRSKRPSITDQEALQFHARGKPGKLEIVPTKPMATQRD
                     LSLAYSPGVAAPVKAIHADPSTAFDYTTRGNTVAVVSNGTAILGLGNLGALASKPVME
                     GKAVLFKRFADIDSIDLEVDTEDPEEFINSIRFLGPSFGGINLEDIKAPECFLIEERL
                     RELMDIPVFHDDQHGTAIIAAAGMINAMKLTGRDIKTTRLVCNGAGAAGIACLDLIKA
                     IGFAPQNVLLCDTKGVVYRGRKEGMNQWKSAHAVETPLRTLEEAVAGADIFFGLSVKG
                     ALTPDMVKSMAPDPIIFAMANPDPEITPEEAHKARNDVIVATGRSDYPNQVNNVLGFP
                     YIFRGALDVRAKTINMEMKIAAANALAELAREDVPDEVAAAYQGARPRFGREYIIPVP
                     FDPRLIYVIPAAVARAAMDSGVAQKPIVDLKAYQNQLSARRDPVAGALQRIMERVRRN
                     PKRVVFAEGEEEQVIRAAYSFATQGLGSVVLVGRENRVRAVADAAGFDLAEKGIEIHN
                     AALSSRNSVYAQFLYERLQRRGFLFRDCQRLINQDRNTFAAAMVAQGDADAMVTGVTR
                     NFSVVLEEVRRVIDHKPGHRVIGVSLVLARGRPVVVADTAIAEMPTAEDLAQIAIEAA
                     GVARRLGYEPRVAMLAFSSFGHPAGERSMRVQEAVRILDQQRVDFEYDGEMAADIALN
                     RAAMAAYPFCRLTDAANVLIMPAFHSASISTKMLQELGGATVIGPLIVGLEKPVQIVP
                     LGARDSDIINMAALASFNIGG"
     gene            complement(6555..10841)
                     /locus_tag="VKU03_04815"
     CDS             complement(6555..10841)
                     /locus_tag="VKU03_04815"
                     /inference="COORDINATES: protein motif:HMM:NF016260.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translocation/assembly module TamB
                     domain-containing protein"
                     /protein_id="HLJ70615.1"
                     /translation="MRARLGVIAALLVALAALAFIIAPLSAAEEDKGVLANLISKALS
                     SPSTKVSIGAVDGALSSDATIRDIVLSDRDGPWLKLDKARLVWSRLALFKRRLEVDQL
                     EIGHLQFLRKPLPAPAGATPPAPTTDAAPADAPILPELPVKVIIKDFGVADLALGAPV
                     VGAEAHLAIKGRATLGPPSEGLDLSLDAHRLDAGGAFVAKLGFVPQSATLNVDVNFDE
                     PAGGLVSTLANLPGAPPVKLSLAGAGPLDKFNAKLAFEAGPSIGANGGVDLTREGPAR
                     RLGLKLQSRVEGLAPSVIRPVFAGETTLDGDMLIRDDSSLTLSGLHLVSANARLDIEG
                     TVGADRALDVKIHAGAIPGAPKIGKLDFNATIAGAASAPKIDAKFDAEKLAFDQGGLD
                     RLIASFSAEPNGALDAPATRIALMGDAAASGLKLADKALDHAVGSDLKLTFRAKASPE
                     GALDVETLQAAATALNLSYTGLLSSEKAKGAVVIEAPDLSHFAALAGKALKGVATVTA
                     DIEGSPKTHALAASFDGHVSNLATGVAGVDGWTGGALTLSGVAESLAGGGFGFRKLAL
                     LGRHGRALLDGAIGADKANLKGEIDAPDARVLDPRILGKATIAGAVTGAGDRLNADVT
                     ASLDDGRLMDRPTSGLALKLNAQDLTGALDARASLSGDIGRQPLQGALHIAKQSDGGW
                     KADGLSFSLGSARLDGDLALAADFLAAGKVAVTIGNFDDLSPLLLTRLAGALRAEIAL
                     TATDGRQNAHIVAKSDNLAFAANSLSGLDADLTATDIYGRKILSGDVKLAKASVSGET
                     IADLSFKASGATDSSDLAVAAKARGLALQARGKLVADAISRFELASLSATGNGQKLSL
                     AHPASFAFGGDGVDIKNFALGINAGRVSVDGHAGAALDLRLKLAAVPLAAADLAKAGL
                     GLQGVADGEATISGAADAPSGDWRVQLKSVSAPQTRSAGLPAVEISGDGRLGDGRTTL
                     DITMKAGAASALRAAGAAPLKSEGDLNLAVAGKLDAGLANGLLSASGRRLSGMVAIDA
                     HLRGPIAKPLAKGSVTMTGGVFSDETIGLKLTAIDALIAADGDQIRIERLKAATSNGG
                     VLNVSGQARLDAEAGFPGSIHIASERAQLVNIDLVAATVDLGLDVTGALARDPKVSGR
                     VAVDSMDISVPERLGGAAAPLQDVKHVNPGETAKARLALDAKGQRGHAAPPFNASLAI
                     VVSAPSRVFVRGRGIDAELGGELKVNGTTAKPQIDGGFEMRRGSLSMLGKRLAFTSGK
                     IQFLGDVIPELDMVAETQATDITARITVTGPASHPSFAFTSDPSLPQDEILSRVLFQK
                     TSGSLSPVQALQLANAAASLAGGGDSFERLRKTLGVDSLDISTDSTGGAAVGVTRAIN
                     DRISVGVKSGAKPQDNGVNLDLDVTRHIRLQGGVDAGGGSSAGVGAQWEY"
     gene            complement(10841..12652)
                     /locus_tag="VKU03_04820"
     CDS             complement(10841..12652)
                     /locus_tag="VKU03_04820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="autotransporter assembly complex family protein"
                     /protein_id="HLJ70616.1"
                     /translation="MAGGDSGLVNAVKDASSLYKLRQDAPPDGDSLARRAMRDFSPIV
                     DALWGAGYYDAKVIIAIDQASLKIGATDTSAFAKAAEAYRNAKAAPIRIAVDPGPLFA
                     LRSVRIVDAASGLAYTRDELPQKIIGLKPGDPAAASDIRAAMARVIDYVRAQGHPLVK
                     VQAPSPIVDHVAHIMDLTLAVAPGPRATLGEATIVGPQHFDPLVARSFLYLEPGDPYS
                     PKALADARTSIRNIPAVGSVRVTEADHLDAQGQLPYQVEVVDRAEHAIGASAAYSTID
                     GPNGQLYWEDRNLFGGAERLRLEGDLLYAPPNDGLVNQYGHFGLADLGWRVSASFLKP
                     ALGGTRNDLLIDALDEHASTNTLNYIGYTVSDVDATAAIRHRFTDAFSIQIGIEGQTG
                     VATDVLGKVDYTIIGVPITAVYDTTDSKLDPTRGVRVGATLAAYPTFLGSTLDLFEAK
                     GHMSTYYSLDEDSRYVLAGRVAAGSLTGSSLDDIPANLRFYSGGGGSVRGYAYDSLGP
                     LGPNNSVIGGRSLFEASAEVRIKVTDTIGVVPFFDAGNAFAANFPDFKDPLQMAAGLG
                     LRYYTAIGPIRVDIATPINRRSGDKPVAVYVSVGQAF"
     gene            complement(13012..14130)
                     /locus_tag="VKU03_04825"
     CDS             complement(13012..14130)
                     /locus_tag="VKU03_04825"
                     /EC_number="2.1.1.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018700521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003871 -
                     5-methyltetrahydropteroyltriglutamate-homocysteine
                     S-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-methyltetrahydropteroyltriglutamate--
                     homocysteine S-methyltransferase"
                     /protein_id="HLJ70617.1"
                     /translation="MTEKPPFRADHVGSYLRPKRLGEAREAFFAGRLPADDLRRIEDD
                     CIREVVRQQENVGLKGITDGEFRRTYFHVDFLEKLDGVTVTYGEFAAKFRKDDGTEVG
                     FRPPTMHVESKIRHATSIQGRDFDFLKAATRQTPKVCIPAPSMLHFRGGRKAISESAY
                     PDLEAFYDDLTAAYRDEIADLAKRGCRYLQLDDTNLAYLCDPAIRESTKARGDDPDAL
                     THLYCRLVNDSIRDRPKDMTISVHLCRGNFKSAWVAQGGYEPVAEILLNEMKIDGFFL
                     EYDDERSGDFAPLRFTPKGKMVVLGLMSSKNRAVESKEEIKKRIEEASHYVPLDQCAL
                     SHQCGFSSTAHGNDISEDDQWKKLARAVEVAREVWGDV"
BASE COUNT         2617 a         4470 c         4634 g         2573 t
ORIGIN      
        1 cggcacgatt accgaggaac ggctcttgga gccgggccgc gccaatcttc tcgtcgcaat
       61 ccatcgcttg cgcgccggcg aggaccgtac tcttttcggc gttgcggcgg tcgatatttc
      121 aaccggcgcc ttttggctga gcgagagcga tgcggcttcg ctcagcgcgg aaattgcgcg
      181 gctggagccg agcgagatca tcgcgacaca ggcgactctg gacgatcccg acgccgcgcg
      241 cgccttcgcc gagacccggg ccgctgtgac gccaatcggg cgcgaagcct cgacggggga
      301 agcggctgag caaaggttgc gggaatttta tggcgtcgaa acgctcgacg gcttcggcgc
      361 tttcagccgc gccgagatcg gcgccgcggc gctggcgatc cattatgtcg ggcgtacgca
      421 gcttgaagcg cgcccggcgc tggagcggcc gtcacgccgt ccgcgcggcg agacgatgga
      481 gatcgacgcg gcgacaagag ccaatctgga gcttactcgt accctgagcg gccagcggca
      541 aggctcgctc ctggcgacga tcgatttgac ggcgaccccc gccggcgcgc ggctccttgc
      601 ggaacggctc gccggcccgc tcactgatcc cgccacgatc aaccggcggc tggacgcggt
      661 tggctatttc atcgaagagc agcgtctgcg cggcgatttg cgcgcgcgcc ttcgcgctgc
      721 gcccgatatc gcgcgggcca tgtcgcgctt gagccttgat cgcgccgggc cgcgcgatct
      781 cgccgccttg cgcgatgggc tcggcgttgc gcgccttgtc gcggctctgc tcgctgcgca
      841 gccgagcggc agtgtgatca tcgccgccgc cgcgcgccgc ctcgctcaaa tggcgcccgt
      901 tctcgaacag gcgctttccg agacgctctc cgacaatctg ccgctcaaca aacgcgacgg
      961 cggcttcgta cgcgaaggcg ctgaaccggc gctcgacgaa gcccgcgcct tgcgcgacga
     1021 gagccggcgg gtcatcgccg aattgcaggg gaaatattgt tctcttgccg agacgaccca
     1081 gatccgcatc aagcacaatc actttcttgg cttcttcatc gagacgccgc aagcgcatgg
     1141 cgagcgcctg ctgaaagcgc catatgcagc gacattcatt caccggcaga ccatggccgg
     1201 cgcgatgcgc ttttcgacaa acgaactggc ggagctcgag gccaagatcg cctcggccgc
     1261 tgatcgcgcc ttggctttgg agctcgctgc cttcgaaagg ctgcgtcagg cgtcgctcgc
     1321 cgaactcgac gcgatccgcg gcctcggcga agcgttggcc gaaatcgacg tcgccgccgc
     1381 tttggccgaa cttgcggcgt cgcgcgattg ggtgcgccct gttgtcgatg attcgttgac
     1441 cttcgtggtc gaggccggcc gtcatccggt ggtcgaggcg gcgctgcagg cgcagggcga
     1501 gcccttcgtc gccaatgatt gcacgctctc cggcgagatc ggcgagggcg gctgcattgc
     1561 gttggtcacc ggcccgaata tggcgggtaa atcgacgtac cttcgccagg gcgccctgat
     1621 agccttgctg gcgcaggcag ggtcttatgc acccgcgcga cgcgtccata tcgggatcgt
     1681 cgaccggctt ttctcgcgcg tcggcgcggc ggatgacctc gcccgcggcc gctcgacctt
     1741 catggtcgag atggtcgaga cggcggcgat cctcaatcag gcgacgcagc gctcattggt
     1801 tattctcgac gagatcggtc gcggcaccgc gacttatgac ggcctctcga tcgctttcgc
     1861 gacgatcgaa catctcaacg cggtcaatcg atcgcgcgcg cttttcgcga cgcattatca
     1921 cgaactgacc cggcttgccg cgaccttgcc gcgcatgacc aaccttgcgc tgcgcgtcac
     1981 ggaatggaaa ggcgatctcg tcttcctgca cgaagtcgcg ccgggcgcgg ccgaccggtc
     2041 gtacggcgtc caggtggcga aactcgccgg gctgccgagc gccgtcgtcg agcgcgcgcg
     2101 gactatcctg gcggaactcg agtcaggcaa tcgttctggc tccgcgttga agaagctcga
     2161 cgatctgccg ttgttttcct acgcgccgaa accgcccgag gccgcgccga agcgcgacgc
     2221 tttgcgggac aagcttgcga cgatcgatcc cgacgagatg acgccgcgcg aagcgcaggc
     2281 ggcgctttat gggttgaagc aattggccaa ggaagacgaa gggtgaatgt cggaccgcgt
     2341 catagcggcc gccgaaggcg tgatcgatag accgccactt gcgcacaagc ggagaggcac
     2401 ggcagtccgt gcaatgaatt gacgcaccgt cgcaatcaac gcaccaaata tcgaacgtgc
     2461 cgcgtactct attgagcgcc cgtcaaatcg aacccggcgg gataaaagcg caatagagga
     2521 aaatatgctc gaagacattc gtggccgctt ggccctgccg gtcgtaagcg gtcgcctggc
     2581 gatagcagag atgagcatga tgcatattgt cgctgggcgg atcgccaggg atcggcaaga
     2641 aggtgatgcg ctcctccggg atcgcgaccc attcattttc gcgggtcagg aactcgaaag
     2701 tccccccatg ctcgcgataa acggtggccg cgcaatcgtc gccaccgcag caaagctggc
     2761 cgttcctgcc atgcacgccg gtgtagattt cgtgagcgcg gctcagcgaa atcggcgcga
     2821 ccacaagcgc gccagcgaaa cccagagaca caagagcttt tatcatcgtc aaccgctcct
     2881 tttcttggcg gccgatcgat cgaggcttca aacgatgcga tcaagccacg cgctgatcgc
     2941 ccttcgtgga acgcttgatc tcccaaaagg ttagacggca gcggcgcagc cgcgcgcatg
     3001 ctcgcttcta tgcggttgat cttcggcgca tatgccgccc aggcgtcccg ctgcggatcg
     3061 ttgcagcgaa gatgcggctt cgagcgcatc ctccagccgc gagaaagcgt tgctaaggcg
     3121 tcgcggcatc cggcaagtcc taagcaaacg gcgtgccgaa tcgcgatata ggagaatccc
     3181 gatggcgatt cgcgtcaagg cgaaattgca ggagcgcgag ggataagggg cggaggtgtt
     3241 tgcgatgcgc aacggcggcc acgtcgccgc gaccccgtca ggccgcttcg tcgctgctcc
     3301 gcttgcgctt cggtgacagc gccgttccga tgcggcttgt ggcggccatg gtctttgcta
     3361 ctgccgcctc gtcacatagg cgctcaacgc cgccgtcggc gaaccttccc ggatcaacac
     3421 gcaatcgtcc ggaaaatcct gccggaatgc cgcacctaga gccaaggcgc cctcgataaa
     3481 tcgctccagg tggaccatcg cgccggtcgg aatatcgtgc agcacgacga ggctttcttc
     3541 ttgctcgccg atctggcgaa gcgccgtatc gacccaaccg taagggtcgc tccaatcgcg
     3601 cggaacaacg ttccaggtga cgcaggtcgc gcgtcgcttc aagagatgat cgaccgccgc
     3661 ctcgctgagc agacgccgat caagcatccc cccttggcca tagggtcgaa acaatggcga
     3721 tggcgacgca taatcgccga tcagcgcatc ggttctttcg atttcttcaa ccgaggcggc
     3781 gggatcctcg ataaggccga gcggggtctc gtgatggtag gtgtgattgc cgatccaatg
     3841 gccgcgcgca tgcgcttcgg ccatcgcggc gcgcccctcc ggcgcctcga tattcttgcc
     3901 gagaacgaag aacgtcgccg gaatggcgta acgcgccaga gtctggagcg ccattggcgt
     3961 gacgccggga tgggggccgt tgtcgaaaga cagcgtgacg agaggcatgg aacaccgtca
     4021 ttgcgaggag cgaagcgacg aagcaaccca ggggcagtcg cggcgtcttg atgcttcact
     4081 tcgttcgcaa cggcgccggg cattttggaa tcgtactctc tcaaccgccg atattgaagc
     4141 tcgccaaggc ggccatattg ataatgtccg agtccctcgc gccgaggggc acgatctgca
     4201 ccggcttttc gagaccgacg atcaatggcc cgatcaccgt cgcgccgccg agctcctgca
     4261 gcattttcgt cgagatcgac gccgaatgga acgccggcat gatcagcaca ttggcggcgt
     4321 ctgtgaggcg gcagaacgga taggcggcca tcgccgcgcg gttcagcgcg atgtcggcag
     4381 ccatttcgcc gtcatattcg aaatcgacgc gttgctgatc gagaatgcgc accgcctcct
     4441 gcacacgcat cgagcgctcg ccggccggat ggccgaacga ggagaaagcc agcattgcga
     4501 cgcgcggctc gtaccctagc cgcctcgcca cgccagccgc ttcgatcgca atctgggcca
     4561 gatcctccgc cgtcggcatc tcggcgatcg ccgtatcggc gacgacgacc ggacggcccc
     4621 gcgccagcac cagcgagacg ccgatcacgc gatggccggg cttatgatcg atgacgcggc
     4681 gcacttcttc caacacgacg gagaaattgc gggtgacgcc ggtgaccatc gcgtcggcgt
     4741 cgccctgcgc aaccatggcg gcggcgaatg tgttgcggtc ctgattgatc aaacgttgac
     4801 aatcgcgaaa gaggaagccg cgccgttgca gccgttcgta aagaaactgc gcatagaccg
     4861 aattgcgcga agaaagcgcg gcattgtgga tttcgatgcc tttctcagcg agatcgaacc
     4921 cggcggcgtc ggcgaccgcg cgcacgcgat tttcgcgccc gaccagcacg accgacccta
     4981 agccctgcgt cgcaaaggaa taggcggcgc ggatcacttg ttcttcttcg ccttccgcga
     5041 agacgacgcg cttgggattg cgccgcaccc gctccatgat ccgctgcaag gcgcccgcca
     5101 ccggatcgcg ccgcgccgaa agctgattct gataggcttt caagtcgacg atcggctttt
     5161 gcgcgacgcc tgaatccatc gccgccctgg cgaccgccgc ggggatcaca tagatcagcc
     5221 gtgggtcgaa aggtacggga atgatatatt cgcgaccgaa gcgcggccgt gcgccttggt
     5281 acgccgcggc gacttcatcc ggcacgtcct cgcgggccag ttcggcaagc gcattggccg
     5341 ccgcgatttt catctccata ttgatcgtct tggcgcgcac gtcgagcgcg ccgcggaaaa
     5401 tgtagggaaa gccgagaacg ttgttgacct ggttgggata gtcggagcgc cccgtcgcga
     5461 cgatgacgtc gtttcgcgcc ttatgcgcct cttccggcgt gatctcaggg tcgggattgg
     5521 ccatggcgaa gatgatcgga tcgggggcca tgctcttgac catatcgggc gtcagcgcgc
     5581 ctttgacgga aaggccgaag aaaatatcgg cgcccgcgac cgcctcctcc agcgtacgca
     5641 aaggcgtttc gaccgcatgc gccgacttcc attgattcat gccttccttg cggccgcggt
     5701 agacgacgcc cttggtgtcg cagagaagga cgttctgcgg cgcaaaaccg atcgccttga
     5761 tgagatcgag gcaggcgatg ccggcggcgc cggcgccatt gcagacgaga cgggtggtct
     5821 tgatgtcgcg gccggtgagc ttcatcgcat tgatcatgcc ggcggcggcg atgatcgccg
     5881 tgccatgctg gtcgtcgtgg aagaccggaa tgtccattaa ttcgcgcagg cgctcctcga
     5941 tcaggaaaca ttccggcgcc ttgatgtctt cgagattgat gccgccgaaa ctcggcccaa
     6001 ggaatctgat gctattgatg aactcctcgg gatcctcggt gtcgacttcg aggtcgatcg
     6061 aatcgatatc cgcgaaacgc ttgaagagaa cggccttgcc ctccatcacc ggcttggagg
     6121 cgagcgcccc gagattgccg aggccaagga tggcggtgcc gttcgagacc accgcgaccg
     6181 tatttccgcg tgtcgtgtag tcgaaggcgg tggaggggtc ggcgtggatc gccttgaccg
     6241 gcgcggcgac gcccggcgaa taggcgagcg acagatcgcg ctgcgtcgcc atcggcttgg
     6301 tcggcacgat ttcgagcttg cccggtttgc cgcgcgcgtg aaattgcagc gcttcctggt
     6361 cggtgatcga agggcgtttg ctacggcctt cggtttcctt ggtcattcga tttcccccaa
     6421 tccgacccgt cttattcgaa aggccagcat aacgggcgat ggcgcggtcg caaattggcg
     6481 gcttaagccc gcatcaaaag gccgcatgcg ggcttcagag acgatcgtta acgccccctt
     6541 cgattgcgag aaaactagta ctcccactgc gccccgacgc cggcgctgga gccgccgccc
     6601 gcatcgacgc cgccttgcag acggatatgg cgcgtgacgt cgaggtcgag gttgacgcca
     6661 ttgtcttgcg gcttggcgcc cgatttgacg ccgacgctga tacggtcgtt gatggcgcgg
     6721 gtcacgccaa ccgcggcgcc acctgtacta tcggttgaaa tatcgagact gtcgacgccg
     6781 agggtcttgc gcaaccgctc gaaggaatcg ccgccgcccg ccagggacgc cgccgcattg
     6841 gcgagttgca gcgcttgtac cggcgaaagg cttcccgaag tcttttgaaa aaggacgcgc
     6901 gagaggatct cgtcttgcgg caggctggga tcggaagtga aggcgaaact cgggtgcgag
     6961 gccggacccg tgaccgtgat gcgcgcagta atgtccgttg cctgcgtctc ggcaaccatg
     7021 tcgagctccg gtatgacgtc gccgaggaat tggatttttc cgctggtgaa ggcgagcctt
     7081 ttgccgagca tcgagagcga cccgcgccgc atctcgaagc cgccgtcgat ctgaggcttg
     7141 gccgtcgttc cattgacttt caattcgccg ccgagttcgg cgtcaatgcc gcggccgcgc
     7201 acgaagacgc ggctcggcgc cgaaacgaca atcgccaagc tggcgttgaa gggcggagcg
     7261 gcatggccgc gttggccttt ggcgtcgagc gccagacggg ccttcgccgt ctcgccggga
     7321 ttgacatgtt tgacgtcttg cagcggcgcc gcggcgccgc caagccgctc cggaacgctg
     7381 atgtccatcg aatccaccgc gacgcggccg gagactttcg gatcgcgggc cagcgcgccg
     7441 gtcacatcaa gcccgaggtc gaccgttgcg gcgacgaggt cgatattgac gagctgagcg
     7501 cgctcgctgg cgatatgaat cgatccggga aagcccgcct cggcgtcgag ccgcgcttgt
     7561 cccgacacgt tcaagacgcc gccattgctc gtcgccgctt tgaggcgctc gattctgatt
     7621 tggtcgccgt ccgcggcgat cagcgcatcg attgcggtca gcttcagccc gatcgtttca
     7681 tccgagaaga cgccgccggt catcgtcacg gatcccttcg ccaagggctt ggcgatcggg
     7741 ccgcgcaaat gcgcgtcgat tgcgaccatg ccggagaggc gtcgtcctga cgccgacagc
     7801 aagccattgg cgaggccggc gtcgagcttg ccggcgaccg ccagattaag atcgccctcg
     7861 ctcttcagcg gcgcggcgcc ggcggcgcgc agagcgctgg cggcgcccgc cttcatcgtg
     7921 atgtcgagcg tcgttcttcc atcgccgagt cggccgtcgc cggatatttc gacggcgggc
     7981 aaaccagcgc tacgcgtctg cggcgcgctg acgcttttaa gctggactcg ccaatcgccg
     8041 ctcggcgcgt ccgcagcgcc gctgattgtc gcctcgccgt cggcgacgcc ctgcaagccg
     8101 agaccggcct tggctaaatc ggccgccgcg agcgggacgg cggcaagttt gaggcgcaga
     8161 tcgagcgccg cgccggcatg gccgtcgacg gagacgcgtc cagcgttgat cccaagcgcg
     8221 aaattcttga tgtcgacgcc gtcgccgccg aaggcgaaag aagcgggatg cgcaagggac
     8281 aatttctgtc cattgccggt cgcgctgaga cttgcgagtt cgaaacgaga gatcgcatcg
     8341 gcgacaagct taccgcgcgc ttgcaaggcg aggccgcgcg ctttagccgc gacagcaagg
     8401 tcgctggaat cggttgcgcc gctcgccttg aaactgagat cggcgatcgt ttcgccgcta
     8461 acgctcgctt tggcgagctt gacgtcgcca gagaggattt tccggccgta gatatccgtc
     8521 gccgtcagat cggcgtcgag acccgacaaa gaattcgccg cgaatgcgag attgtcgctc
     8581 ttggcgacga tgtgggcgtt ctgccgcccg tcggttgcgg tcaaggcgat ctcggcgcgc
     8641 agggcgccgg ccagccgcgt caggagcagc ggcgagagat cgtcgaaatt gccgattgtg
     8701 accgcgacct tgccggcggc gagaaaatcc gcggcaaggg cgagatcgcc gtcgagccgc
     8761 gccgagccga gggagaacga aagcccatcc gccttccagc cgccgtcgct ctgtttggcg
     8821 atatggagcg cgccctgcag cggctggcgg ccgatgtcgc cgctgagcga agcgcgcgcg
     8881 tcgagggcgc cagtcagatc ctgcgcgttc aatttcaagg cgaggccgga cgtcggtcgg
     8941 tccatcaggc ggccatcgtc gaggctcgcg gtcacgtctg cgttgagccg atcgcctgcg
     9001 cctgtgacag cgccggcgat cgtcgccttg ccgagaatgc gcgggtcgag aacacgcgcg
     9061 tccggcgcgt caatctcgcc tttgagattg gccttatcgg cgccgatcgc gccgtcgagc
     9121 aaggcgcgtc catgcctgcc gagaagcgcc agcttgcgaa agccgaaacc gccgccggcc
     9181 agactctcag ccacgccgct taacgtcagc gcgccgccgg tccatccgtc gacgccggcg
     9241 acgcccgtcg caagattgct gacatggccg tcaaagcttg cggcgagcgc atgggtcttc
     9301 ggcgagcctt cgatgtcagc cgtgaccgtc gcgacgcctt tgagcgcttt tcccgcaagc
     9361 gcggcaaaat gcgagagatc gggcgcttcg attacgaccg cgcccttcgc cttctcgctg
     9421 ctcagcagcc cggtatagga gaggttcaaa gccgtggcgg cggcctgcaa agtttcgaca
     9481 tcgagcgcgc cttccggcga ggccttggcg cggaaggtca gcttgaggtc ggagccgacg
     9541 gcgtgatcga gcgccttgtc ggcgagcttc agtccgctcg ccgccgcatc gcccataagc
     9601 gcgatacgcg tcgccggcgc gtcaagcgcg ccattcggtt cggcgctgaa cgaggcgatc
     9661 agccgatcga ggccgccttg atcgaaggcg agtttctcgg cgtcgaattt cgcgtcgatc
     9721 ttcggcgcgc tcgccgcgcc tgcgatcgtg gcgttgaagt cgagcttgcc gattttcggc
     9781 gcgccgggga tggcgccggc gtggattttc acatcaagcg cgcgatcggc gccgaccgta
     9841 ccttcgatgt cgagcctggc gttggcggaa acgaggtgaa ggccggagag cgtcaagctc
     9901 gaatcgtcgc ggatcagcat atcgccgtcg agcgtcgttt cgccggcaaa gaccggccgg
     9961 atcaccgagg gcgcgagacc ttcgacgcgc gattgcagtt tcaacccaag ccgtcgcgcc
    10021 ggtccctccc gcgtgagatc gacgccgcca ttcgcgccaa tgctcgggcc ggcctcaaag
    10081 gcgagtttgg cgttgaactt gtcgagcggg ccggcgccgg cgagcgaaag cttcaccggc
    10141 ggcgcgcccg gcaaattggc gagcgtcgag acaaggccgc cggccggctc gtcgaaattc
    10201 acgtcgacat tcagcgtcgc gctctgcggc acgaagccga gtttcgccac aaaggcgccg
    10261 ccggcgtcga gccgatgcgc gtcgagacta agatcgagcc cttcggacgg cggcccgagc
    10321 gtcgcgcgtc ctttgatcgc aagatgcgct tcggcgccga caaccggcgc gccgagcgcc
    10381 agatccgcga cgccgaaatc cttgatgatc actttgaccg gcagttccgg caggatcggc
    10441 gcatccgccg gcgccgcgtc cgtcgttggc gccggcggcg tcgcgccggc gggcgcgggc
    10501 aatggtttgc ggaggaattg cagatggccg atttcgagtt gatcgacctc caggcgccgc
    10561 ttgaacaaag ccagacggct ccacaccagc cgcgccttgt cgagcttcag ccagggcccg
    10621 tcgcgatccg acaggacgat gtcgcggatc gttgcgtcgg acgacagcgc gccatcgacc
    10681 gcgccgatcg atactttcgt cgacggcgac gagagggctt tggagatgag attggcgaga
    10741 acgcctttgt cttcctcggc ggcgctgagc ggcgcgatga tgaaggccag cgccgccaga
    10801 gcgacaagca gcgcggcgat gacgccaaga cgcgcccgca tcagaaagcc tgcccgacgc
    10861 tgacatagac cgcgaccggt ttgtcgcccg aacgccgatt gatcggcgtc gcaatatcga
    10921 cgcggatcgg cccgatcgcc gtgtagtagc gaagcccgag acccgcggcc atttgcaacg
    10981 ggtccttgaa gtcagggaaa ttcgcggcga aggcgttgcc ggcgtcgaag aacggcacga
    11041 cgccgatcgt atcggtgacc tttatgcgca cttccgccga ggcttcgaaa aggctgcggc
    11101 cgccgatgac ggaattgttc ggtccgagcg gaccgagact gtcataggca tagccgcgca
    11161 ccgaaccgcc gccgccggaa tagaagcgca agttcgccgg aatgtcgtcg agcgacgagc
    11221 ccgtcaatga accggcggcg acgcggccgg ccaacacgta gcgcgaatct tcatcgagcg
    11281 aataataggt ggacatatgt cctttcgcct cgaagaggtc gagcgtcgag ccgagaaagg
    11341 tcggataggc ggcgagcgtg gcgccgacgc gaacgccgcg cgtcggatcg agcttgctgt
    11401 cggtcgtatc atagacggcg gtgatcggaa cgccgatgat cgtataatcg accttgccca
    11461 gcacatcggt cgcgacgccg gtttgtccct cgatgccgat ctggatggag aaagcgtccg
    11521 taaaacgatg gcggatcgcc gccgtcgcat cgacgtcgct gaccgtatag ccgatatagt
    11581 tcagcgtgtt ggtgctggcg tgttcgtcga gcgcgtcgat gagaaggtcg ttccgcgttc
    11641 cccccaaagc gggctttagg aaactcgcgc tgacgcgcca gccgagatcg gcgaggccga
    11701 aatgaccata ttgattgacg agcccgtcat tgggcggcgc atagagcaga tcgccttcga
    11761 gacgcagacg ctccgcgccg ccgaataagt tgcggtcctc ccaatagagc tggccattgg
    11821 gaccatcgat cgtcgaataa gccgccgagg cgccgattgc atgctctgcg cggtcgacaa
    11881 cttcaacctg atacggcaat tggccttgcg catcgagatg atcggcttcg gtgacacgca
    11941 ctgagccgac ggcaggaata ttgcgaatac tggtcctggc gtcggcgagg gctttcggcg
    12001 aatagggatc gcctggctcg agataaagaa aagaacgcgc gaccaaggga tcgaagtgct
    12061 gcggcccgac aatcgtcgcc tcgcccaatg tcgcgcgcgg gccaggcgcg acggcgagag
    12121 tcaaatccat gatatgggcg acatggtcga cgattggcga cggcgcctgc actttgacga
    12181 gcggatggcc ttgcgcgcga acgtaatcga tgacgcgcgc catggcggcg cgaatgtcgc
    12241 tggccgcggc cggatcgccg ggtttaagcc cgatgatctt ttgcggaagt tcgtcccgcg
    12301 tataagcaag tccgctcgcc gcgtcgacga tgcgaaccga tcgcaaagcg aaaaggggac
    12361 ctggatcgac cgcaatccgg atcggagcgg ccttggcgtt gcgataggct tcggcggcct
    12421 ttgcaaaggc gctcgtatct gtcgcgccga tcttcaggct cgcttgatca atggcgatga
    12481 tgaccttggc gtcgtaataa ccagcgcccc acagggcgtc gacgatcggc gaaaaatcgc
    12541 gcattgcgcg ccgcgccagg gaatcgccgt caggcggcgc gtcctggcgc agtttataga
    12601 gcgatgacgc gtccttgacg gcgttgacca gtccggaatc gccgccggcc acatcgatcg
    12661 aaactttgta ggcaattgcc gtttgtgaga cgggcggcgg cgcgtcatcg ccccaaaagc
    12721 cgaaaaaatc aaaagcggaa gcgcttgtcg tcagacaagc gaccggcgcc aacgcgacgc
    12781 tcaacgcgag aaatacgcga acctttcgac caataaacaa cgtgacgtcg cccctgcaaa
    12841 ttccgaggac gttgttggct tatttcatca actttggacg tcggcgataa gacttgtcgc
    12901 cggcgatgag gacattgcga ttaaataacg gtcaccctga gtgaatgaaa tttccgacca
    12961 aagtcttaac gcggcgatgc cgatcgccgc aaggcgccgc gccaaagagg cttaaacatc
    13021 gccccaaact tcacgcgcca cttcgacggc gcgcgcgagc ttcttccatt gatcgtcttc
    13081 gctgatgtcg ttgccatgag cggtcgagga gaagccgcat tgatggctca gcgcgcattg
    13141 gtcgagcggc acgtaatgac tcgcctcttc gatgcgcttc ttgatctctt ctttcgattc
    13201 gaccgcgcga tttttggagc tcatcaggcc gagcacgacc atcttgccct tgggcgtgaa
    13261 gcgcaaaggc gcgaaatcgc cggagcgttc atcgtcatat tcaaggaaga aaccgtcgat
    13321 cttcatctca ttcagcaaaa tctcggcgac cggctcatag ccgccttgcg ccacccaggc
    13381 gcttttgaaa ttgccgcggc agagatggac ggagatcgtc atgtcctttg gccgatcgcg
    13441 gatcgaatca ttgacgagac gacaatagag atgggtcagc gcatcgggat catcgccgcg
    13501 cgccttggtc gattcgcgga tcgccggatc gcagagatag gcgaggttgg tgtcgtcgag
    13561 ctgaaggtag cggcagccgc gcttagcgag atcagcgatc tcgtcgcggt acgcggcggt
    13621 gagatcgtcg tagaaagcct cgagatcggg ataggcgctc tcgctgatcg ccttgcggcc
    13681 gccgcggaag tgcaacatcg acggcgccgg gatacagact ttcggggtct gacgcgtcgc
    13741 cgctttgaga aaatcgaagt cgcggccttg gatcgaggtc gcgtggcgga ttttggattc
    13801 gacatgcatg gttggcggac gaaagccgac ctcggttcca tcgtccttac ggaatttggc
    13861 ggcgaattcg ccgtaagtca cggtgacgcc gtcgagcttt tcgaggaagt cgacatgaaa
    13921 atacgtacgg cggaattcgc cgtcggtaat cccctttaag ccgacgttct cctgctggcg
    13981 aaccacttcg cggatgcaat cgtcctcgat ccgccggagg tcgtcggcgg gaagccggcc
    14041 agcgaaaaag gcttcgcgcg cctcgcctaa gcgtttcggt cggagatagc tgccgacatg
    14101 atcggcgcga aacgggggct tttcggtcat gcgtctcctc ctggcgaact tgccatcttt
    14161 tctagaggag gggaggcaaa tggcaaccgc ggcggctttc gagcgtgcaa gcgtaggttg
    14221 gcgcgaagga gaaggggtca gagcctcgtc gtcgcccctt cgatcgcgtc gtcaaccgcc
    14281 tgtgcaaatc gctc
//
LOCUS       DATGJW010000158         6824 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_340897, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000158 DATGJW010000000
VERSION     DATGJW010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6824)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6824)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6824
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_340897"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1972
                     /locus_tag="VKU03_04830"
     CDS             <1..1972
                     /locus_tag="VKU03_04830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018457283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ70618.1"
                     /translation="VFERRLAQAPLLVVVEDLHWADAVSLEALRFVVDRLERTRLMLL
                     VTHRPAAEIGQFNSNRVGQSALRLAPLAAADGQRLLAAFFGEDRTGCFADLRSRILQR
                     SGGNPLFVEEIVRNLVEIGALRREGSQWLVADEAATDIPANIEALLLARLDRLPREVR
                     GLVQEAAVIGPRFDASVLKAVAAEPSEVEAGLDILCDAELIEEAASAQAASGRSYRFT
                     QTLQHDVIYHNLLLRRRTDLHGLIGAALIRLHGDNPERLEDATLLGHHLSLSAEKLKG
                     ALYLRMAGDRARAAYANDDAAHLYQQALAVLQAIGERGPEWLALHERLADLYGRSGRR
                     ELAQEHHHAALEAGRAAGERAAAARNLRKLGRLLWDGGKRGQADECCAEAAALLEGAD
                     APVEQAHLLQERGRVAFRLGAHAEAAKWADEALGYAQAATADADGSAAAEAALAVAEA
                     LNTKGAALARLGRTAEAVREVERSVAAAEAANLLGAACRGYTNLGVLYTIIDPARAIE
                     VCRRGLDVARRIGDLGFQARLLSNLAVACCTFTNRCDAEGVPAAEKAIEIDRALDQRE
                     HLPVPLIVLGQIHQCHGHPELAARYYNEALEVALKTGEPQQLFPCYDGLATVSLDQGD
                     EAEAERYFALAQDVCARHGVDPEGLIVLPFLD"
     gene            2005..2685
                     /locus_tag="VKU03_04835"
     CDS             2005..2685
                     /locus_tag="VKU03_04835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin-like family protein"
                     /protein_id="HLJ70619.1"
                     /translation="MAEHHAEGPLQPGDRAPNIVLDAITRDGKIALDDFRGRTPVLVG
                     LFRGLHCPFCRRHIAAMARLDPALRDRGVETFAVVNSPTERARLYYRYHPLPNLLAAA
                     DPERASHRAFGLPMLEFTENETVWPRKVGMDVVLATRVNVPGELDQPMNPIEAGTIMN
                     QRDGYEISEAERKMLPASNGQLVGEFLLDREGIIRWSFTEVAEGGRNMFGAPTPDELM
                     SAAAQVAR"
     gene            complement(2925..3674)
                     /locus_tag="VKU03_04840"
     CDS             complement(2925..3674)
                     /locus_tag="VKU03_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ70620.1"
                     /translation="MPLGLTIFFFDVVLIVHTAKTGRFWPWAYLILFLPGIGGVAYVV
                     AELAPEWLGSYKGRQARQKVAATLNPMGRYRQLRDELFVADTIANRAALAEECLGLGK
                     FDEALAQYELILAKPMGDEPSFMLGKARAEFAMGKAEIAVMTLEELKRHWPDYQSADG
                     HLLYAMALERAGREADALAHYADVGRYYPGAEPRVRQAELLQRLGRVAEASALAEDVV
                     RTLSRAPGHVQRSQKEWLAIAQRVARAQAAH"
     gene            complement(3835..4638)
                     /locus_tag="VKU03_04845"
     CDS             complement(3835..4638)
                     /locus_tag="VKU03_04845"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLJ70621.1"
                     /translation="MPFLSRIFRASIRNEGELTRFVVAVTLICVAIALAVDVTNQLAF
                     FIDWATCLRSWTITTVLVLALAVPISRTIGKAHLELYRAKLLADQLGRTDQLTGLANR
                     RALMEATGGDKPGALALVIVDIDRFKRVNDSYGHRAGDEVIRSVAQTMAAELGEFGLV
                     ARVGGEEFALLAAHAPTDAIESKLQAFRDRVAAAPVLALGQAVRVTISAGLATRRADE
                     SFDQLYSAADRALYAAKAAGRNRVLSADALSTLAERGPAADQDRLPRSA"
     gene            complement(4809..5774)
                     /locus_tag="VKU03_04850"
     CDS             complement(4809..5774)
                     /locus_tag="VKU03_04850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HLJ70622.1"
                     /translation="MRLHRLWLESDPQAALDRLAPKIKAIAATGHLAIVDEKLMRRLP
                     KLEIVASFGVGYDNVDAQWAGEHGIIVTHTPGVLDEEVADTAFGLLIAAARQLPQAER
                     YLREGRWLHAPFPLTPSLRGRTMGILGLGRIGKAIARRATAFGLDVVYHGRKPQDGAP
                     YLYYPTLTGMARAVDILMVVAPGGEETKNIVNAEVLEALGPNGILVNVARGSLVDEDA
                     LIAALNSRKILAAGLDVFADEPKVPAGLLAAENAVLLPHVGSASRATRQAMADLVVAN
                     LYSWLDGKGPLTPVPETPWPRLSSPAKAGEGDHAKHGGGGSGLSG"
     gene            5983..>6824
                     /locus_tag="VKU03_04855"
     CDS             5983..>6824
                     /locus_tag="VKU03_04855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLJ70623.1"
                     /translation="MRNGAKPGAPRRRPILRRVILKDFRSYAALDLPLSGRLIALTGE
                     NGAGKTNLIEALSLFTPGRGLRRAELAECARQNGSGGFAVSILVEDGETTHQLGTGLD
                     REEGEQAPGRKHRVDRVPAVSARAFADYLRIVWLTPSMDGLFAGPAGERRRFLDRLVL
                     AVDPSHGGRVAQLERALRGRNRLLEEGGRDINWLDAIERKTAELAVAVAAARGECVAR
                     LKAMIADERDEDSPFPWADLALDGEVERLASELPALAAEERYREILRDNRGRDAAAGR
                     ALIGP"
BASE COUNT         1103 a         2335 c         2331 g         1055 t
ORIGIN      
        1 cgtgttcgag cgccggctgg cgcaagcgcc gctcctcgtc gtggtcgaag acctgcactg
       61 ggcggacgcc gtgtcgctgg aggcgctccg cttcgtcgtc gaccggctgg agcgaacccg
      121 cttgatgctg ctcgtcactc accgaccagc ggccgagatc ggccaattca attcgaaccg
      181 cgtcgggcag agcgcgctcc gcctcgcgcc gctcgccgcc gccgacggac aaaggctgct
      241 cgccgcgttt ttcggcgagg atcggacggg ttgctttgcg gatttacgca gccgcatcct
      301 gcagcgctcg ggcggcaacc cgctcttcgt cgaagagatc gtccgcaatc tcgtcgagat
      361 cggcgcgctg cggcgcgagg gctcgcaatg gctcgtcgcc gacgaagccg cgaccgacat
      421 tccggcgaat atcgaggccc tgttgctcgc ccgcctcgac cgcctgccgc gcgaggtccg
      481 cgggctcgtg caggaagcgg cggtgatcgg cccgcgcttt gacgcctccg tgctcaaagc
      541 cgtcgctgcg gagccgtccg aggtcgaggc cgggctcgac atcctctgcg acgccgagct
      601 gatcgaagag gcggcgagcg cccaagccgc ttccgggcga agctatcgct tcacccaaac
      661 gctgcagcat gacgtgatct atcacaatct gctgctgcgg cgacgcaccg atctccacgg
      721 cctaatcggc gccgcgctga tccgcctgca tggcgacaat cctgagcggc tcgaagacgc
      781 gacgctgctc ggccatcatc tgagcctcag cgccgagaag ctcaaaggcg cgctgtacct
      841 gcggatggcg ggcgaccgcg cccgcgccgc ctacgccaat gacgacgccg cccatcttta
      901 ccagcaggcc ctggcggtgt tgcaggcgat cggcgagcgc ggaccggaat ggttggcgct
      961 tcacgagagg ctcgccgatt tgtacggccg ctccggacgg cgcgagctcg cccaggagca
     1021 ccaccacgcc gcgctcgaag caggccgcgc cgcgggcgag cgcgcggcgg cggcgcgcaa
     1081 tctgcgcaag cttggccgct tgctctggga cggcggcaag cgcggccagg cggacgaatg
     1141 ctgcgccgag gcggccgccc tgctcgaggg ggccgacgcg ccggtcgaac aggcgcatct
     1201 cctgcaggag cgcggccgcg tcgccttccg cctcggcgcc catgccgagg cggcgaaatg
     1261 ggccgacgag gcgctcggct atgcgcaagc ggcgacggcg gacgccgacg ggagcgccgc
     1321 ggcggaagcc gcgctcgcgg tcgccgaagc gctcaacacc aagggcgccg cgctggcgcg
     1381 gctcggccgc accgccgagg cggtgcgcga agtcgagcga agcgtcgccg ccgcggaggc
     1441 ggcgaacctc ctcggcgccg cctgccgcgg ctacaccaat ctcggcgtgc tctatacgat
     1501 catcgacccg gcgcgggcga tcgaggtctg ccggcgcggc ctcgacgtgg cccggcgcat
     1561 cggcgatctc ggctttcagg cgcgcttatt gtccaatctc gcggtcgcct gctgcacctt
     1621 caccaatcga tgcgacgccg agggcgtgcc ggcggctgaa aaagccatcg agatcgaccg
     1681 cgccctcgac cagcgcgagc atcttcccgt cccgctgatc gtgctggggc agattcatca
     1741 atgccacggc catccggaac tcgccgcccg ctattacaac gaggcgctcg aagtcgcgtt
     1801 gaaaaccggc gagccgcaac agcttttccc gtgctatgat ggcctggcga cagtgagcct
     1861 cgaccaaggc gacgaggcgg aagcggaacg ctatttcgct ttggcgcaag atgtttgcgc
     1921 gcgtcacggc gtcgatccgg aagggctgat cgtgctgccg tttctcgact agctcttctt
     1981 cgcgaaagcg atgggaggat tatcatggcc gagcatcatg ccgaggggcc gctgcaaccg
     2041 ggcgatcgcg cccccaacat cgtgttggac gcgatcaccc gcgacggaaa aatagcgctg
     2101 gacgactttc gcggccgcac gccggtgctt gtcggcctgt tccgcggcct gcattgcccg
     2161 ttctgccgcc gccacatcgc cgccatggcg cgactcgatc cggccttgcg cgaccggggg
     2221 gtcgagacgt tcgcggtggt caatagcccg accgagcgcg cccggctcta ttaccgctat
     2281 catcccctgc ccaacctgct cgccgccgcc gatccggagc gcgcctcgca ccgcgccttc
     2341 ggcttgccga tgctggaatt caccgagaac gaaaccgttt ggccgcgcaa ggtcggcatg
     2401 gacgtggtgc tggccacccg ggtcaatgtg ccgggcgagc tcgatcagcc gatgaatccg
     2461 atcgaggcgg ggacgatcat gaaccagcga gacggttacg agatcagcga ggccgagcgg
     2521 aaaatgctgc ccgccagcaa cggccagctc gtcggcgaat tcctgctcga tcgcgaggga
     2581 atcatccgct ggagcttcac cgaagtcgcc gaaggcggcc gcaacatgtt cggcgcgccg
     2641 acgccggacg agctgatgtc ggccgccgcg caggtcgcgc gctgagcggc gccgcgcagg
     2701 tcgcgcgctg agcggcgccg cgcgtcagca agtatgactc gctttgatac cgcggcgcca
     2761 cgccggccga acggccggct tcgtcagcaa aacgtcacga tagcaacagc gccatcggcg
     2821 cggcgctggg ctttgccgca tcccgcggtc aatccattca tagggcgccg ctctacgaat
     2881 ggattgcttc gtcgcttcgc tcctcgcgat gacggagacc gcgctcaatg cgccgcctgc
     2941 gctcgcgcca ctctctgcgc gatcgccagc cattccttct ggcttcgctg cacgtggccc
     3001 ggcgcccggc tcagggtccg caccacgtct tcggccagcg cgctcgcctc ggcgacgcgg
     3061 ccgaggcgct gcagcaactc ggcctgccgc acgcgcggct cggcgccggg atagtagcgg
     3121 ccgacatcgg cgtagtgggc gagcgcgtcc gcctcgcggc cggctctttc cagcgccatg
     3181 gcgtagagca gatggccgtc cgccgattga tagtcgggcc agtggcgctt caattcttcg
     3241 agcgtcatga ccgcgatctc ggcctttccc atcgcgaatt cggcgcgcgc cttgccgagc
     3301 atgaagctcg gttcgtcgcc catcggcttt gcgaggatga gttcgtactg cgccaacgcc
     3361 tcgtcgaatt tgccgaggcc gagacattcc tcggcgagcg cggcgcggtt ggcgatcgta
     3421 tcggcgacaa aaagctcgtc gcgcagttgt cggtagcggc ccatcggatt gagcgtcgcg
     3481 gcgaccttct ggcgcgcttg ccggccttta tacgatccaa gccattccgg cgcgagctcc
     3541 gccacgacgt aagcgacgcc gccgattccc ggcaaaaaga ggatcaggta cgcccaaggc
     3601 caaaagcggc cggtcttcgc cgtatggaca atcaggacca cgtcgaagaa gaatatcgtc
     3661 aaaccgagcg gcacatgctc ctcccgcgat cttgccgacg cgcgaacaaa gtcgatccga
     3721 cgtcacggcg ctcaatttta gcgacgggcg tttggaaatg tcgaccatga cgatgggtca
     3781 gcgcggaagg ttttttgaga ggtacgatga cggagaaaat gacgctacgt catttcacgc
     3841 gctgcgcggc agccggtctt gatcggcggc tgggccgcgc tcggcgagcg ttgacagcgc
     3901 gtcggccgag aggacgcgat ttcgtcccgc cgccttggcg gcgtaaagcg cgcgatcggc
     3961 cgccgaatag agctggtcga agctttcgtc ggcgcggcgt gtcgccaagc cggccgagat
     4021 cgtcacgcgc accgcctggc cgagcgccag gaccggcgcc gccgcgacgc ggtcgcggaa
     4081 agcctgcagc ttgctttcga tcgcgtcggt cggcgcatgc gccgcgagca gcgcgaattc
     4141 ctcgccgccg acccgcgcga caaggccgaa ctcgccgagc tcggcggcca tggtttgcgc
     4201 gaccgagcgg atgacctcgt cgccggcgcg atggccgtag gaatcgttca cccgcttgaa
     4261 gcggtcgatg tcgacgatca ccaaggccaa agcgcccggc ttgtcccctc cggtcgcctc
     4321 catcaaagcg cggcgattgg cgaggccggt caactgatcg gtgcggccga gttggtcggc
     4381 gagcagcttg gcccggtaga gctccagatg cgccttgccg atggttcgcg agatcggaac
     4441 ggcgagcgcc aggaccagaa ccgtcgtgat cgtccaggag cgcaggcagg tcgcccagtc
     4501 gatgaagaag gcgagctggt tcgtcacatc caccgccagc gcgatggcga cgcagatcaa
     4561 ggtgacggcg acgacgaagc gcgtgagctc gccttcgttg cggatcgagg cgcggaatat
     4621 tcgagaaaga aagggcatga gggcgcgcct cgccggatcg ctagcggcag caaacgccat
     4681 atgaggcgag agaagataag ggcgggttaa ggaattgccc ggcccgctta tcctcccccg
     4741 tgaagcgaag cgaaaccggg gagggggacc gcccgaaggg cggtggaggg ggccgcggag
     4801 tttggggatt atcctgaaag cccggagccc cctccaccat gcttcgcatg gtccccttcc
     4861 cccgctttcg caggggagga gaggcgcggc cacggcgtct ccggcaccgg cgttagcggg
     4921 cctttgccgt cgagccagga atagagattg gcgacgacga gatcggccat ggcttggcgg
     4981 gtcgcgcgcg aggccgagcc gacatgcggc agcaggacgg cgttttcggc ggcgagaagc
     5041 cccgccggca ctttcggctc atcggcgaaa acatcgagcc cggcggcgag aattttccgg
     5101 ctgttgagcg ccgcgatcaa ggcgtcctca tcgaccagcg agccgcgcgc gacattgacg
     5161 agaatgccgt tcgggccgag cgcttcgagc acctcggcat tgacgatatt cttggtctcc
     5221 tcgccgcccg gcgcgaccac catcaaaatg tcgacggccc gcgccatgcc ggtcagcgtc
     5281 ggatagtaca gatagggcgc gccgtcctgc ggcttgcggc cgtgatagac gacgtcgagg
     5341 ccgaacgccg tggcgcggcg cgcgatcgcc ttgccgatcc ggccgaggcc gagaatgccc
     5401 atcgtgcggc cgcgcaggga cggcgtgagc gggaacggcg cgtgcagcca gcgcccctcg
     5461 cgcaagtagc gctcggcctg cggcaattgc cgcgccgccg cgatcagcag gccgaacgcg
     5521 gtgtcggcga cctcctcgtc gagaacgccg ggcgtgtggg tgacgatgat cccatgctcg
     5581 cccgcccatt gcgcgtcgac attgtcgtag ccgacgccga aactggcgac gatttcgagc
     5641 ttcggcagcc gacgcatcag cttttcgtcg acgatcgcca gatggccggt cgccgcgatc
     5701 gccttgatct tcggcgcgag gcgatcgagc gccgcttgcg ggtcgctctc cagccacagc
     5761 cgatgcagcc gcatgcgcgc ttcaatgccg gcggccacgt cgccgatccc gaggatcggc
     5821 atcagcacgt caaaatccgc gatctcgttc gccatgtgtc atgattcccg attgccggcc
     5881 gcatattggc cgggcggagc cggccggcgc aacaggccgg attgccggct tccgcgaagc
     5941 cggcgaacat ggcatggcct tgccatgcca gccgaggaat ccttgcggaa tggggcaaag
     6001 ccgggcgcgc cgcgccgccg gccgattctg cgccgggtca tcctcaagga ctttcgctcc
     6061 tatgccgcgc tcgacttgcc gctctccggc cgcctcatcg cgctgaccgg cgagaacggc
     6121 gccggcaaga ccaacctgat cgaggctttg tcgctgttca cgccgggccg cggcctgcgc
     6181 cgcgcggaac tggccgagtg cgcccggcaa aacggctcgg gcggcttcgc ggtttcgatt
     6241 ctcgtcgagg acggcgagac gacccatcag ctcggtaccg gcttggaccg ggaggagggc
     6301 gagcaggcgc ccggccgcaa gcaccgcgtc gaccgcgtcc cagccgtctc ggcccgcgct
     6361 ttcgccgact atctgcggat cgtctggctg acgccgtcaa tggacgggct attcgccggc
     6421 ccggccggcg agcgccggcg ctttcttgac cgcctggttc tggcggtcga tccgagccat
     6481 ggcggccggg tcgcgcaatt ggagcgggcg ttgcgcggcc gcaaccggct gctcgaagag
     6541 ggcggccgcg acataaattg gctcgacgcg atcgagcgca agacggccga actcgccgtc
     6601 gcggtggcgg ccgcgcgggg cgaatgcgtg gcgcggctga aggcgatgat cgccgacgag
     6661 cgcgacgagg actcgccctt cccctgggcc gatctcgccc ttgacggcga ggtcgagcgc
     6721 ctggcgagcg agctgcccgc cttggcggcg gaggagagat accgcgaaat cctacgcgac
     6781 aatcgcggcc gcgacgccgc cgccggccgc gccttgatcg gccc
//
LOCUS       DATGJW010000159         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_754927, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000159 DATGJW010000000
VERSION     DATGJW010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_754927"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            332..1516
                     /locus_tag="VKU03_04860"
     CDS             332..1516
                     /locus_tag="VKU03_04860"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLJ70624.1"
                     /translation="MSDSRPGSAEARASDHSDIIYPSAIPFILAHLACFAAVWTGVTW
                     RAAALALALYWIRIFAIGAGYHRYFSHRAYETSRIFQFVLAFMAQTTAQKSVLWWAAK
                     HREHHLHSDTELDVHSPRQTGLLYSHVGWIFARRNDGADLVKVADLARYPELMWLHRF
                     ELAPAVALALACFGLAGWPGLVVGFFWSTVAVYHATFCINSLAHCIGRKRYLTGDDSR
                     NNWLLAFFTMGEGWHNNHHAFQSSVRQGFLWWEYDATFYLLKLLAKLGLIWRLKLPPA
                     AVLENRHRVGARIVQRAAAQVADSFNAERIAGLIAATLGKAGLSRLHARLLDARLRMA
                     EVLSEMDLPELPSRTDIVKRARSMMAINAQSIEEIGARAHQLLLEAVGSRLAGLAAQS
                     AE"
     gene            complement(1575..2492)
                     /locus_tag="VKU03_04865"
     CDS             complement(1575..2492)
                     /locus_tag="VKU03_04865"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhopane nucleosidase"
                     /protein_id="HLJ70625.1"
                     /translation="MFIGNHIGEETTAGATRDAYAAGEAEPSLVADADSRRFNWRELW
                     PISHRLRSYLDGKIWPDWEPAWRWPDFFAEGGREASLWRPAPPSSDILAIVGLQFEAR
                     ILASRSIRAVFLRPDPNLLSANIIAANRGIISFGICGGLAPGLRAGACIIASSIHDGA
                     RIWRTNEEWTRRLLQSIPGAIVAPILGVDAPVIHIAEKSRLYERFGAAVVDMESHLAA
                     AAASAHRLPLAAVRVVADDARCGLPDAALAGRRVDGSIDAPAVLRALLDNPSDLAPLI
                     RLAAQTHLARAKLLSLSQSLGGRMGLPFP"
     gene            complement(2740..3582)
                     /gene="hpnK"
                     /locus_tag="VKU03_04870"
     CDS             complement(2740..3582)
                     /gene="hpnK"
                     /locus_tag="VKU03_04870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317975.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid biosynthesis-associated protein HpnK"
                     /protein_id="HLJ70626.1"
                     /translation="MNRLVVTADDFGLAREVNEAVEIAHRKGILSAASLMVAAPEAQD
                     AVSRARSMPNLRVGLHLALTDALPALPPEKIPQLVDRAGRLRADLARLGFDLAVDRDA
                     RRQMRAEIEAQFAAFRATGLALDHVNVHQHFHLHPVVAAMVIGIGRAYRMRALRAPYE
                     PRRMIALADESSAPAYRAVEQGCAAWLRRLAKRAGLATADGVLGLRWSGCMTAQRLQS
                     LLSRLPPGFWEIYLHPATRDAFPGCAPGYRYVDELAALVDPLSIEALRRAGRSIGGYG
                     DLPG"
BASE COUNT          649 a         1178 c         1216 g          592 t
ORIGIN      
        1 cagcgattgt ctggccgacc ttcagagatc gtgagcgatt tgccagaaac gcggcagcat
       61 cgagattttg gacgctgaaa cgatcgcgcg ggaggcttgc gaatgccacc gcgtcatccg
      121 cgccgaattt acccatttgt ggagtagaca accggggaac gcccgcgcct cggtcgcgaa
      181 cggggccggg gcgctcagtc tcttcgcgcg ggagcgggcg gagaatcagg ccgaccgata
      241 aaataagcga agcgaaccgt acaaggtcat tcttttctta cgctggcgac cgccatcatt
      301 tggtcgcaag cggcggcgaa ggatcaaggc catgtcagat tcccgtccag gctccgccga
      361 agcccgcgct tccgaccatt ccgatatcat ctatccgtcg gcgatcccgt tcatcctggc
      421 gcatctggcc tgtttcgccg ccgtctggac gggcgtcact tggcgggcgg ccgcgctcgc
      481 ccttgcgctc tattggatca ggatattcgc catcggcgcc ggctatcacc gctatttttc
      541 gcatcgcgcc tatgagacga gccggatttt ccaattcgtc ctggcgttca tggcgcaaac
      601 gaccgcgcag aaaagcgtac tgtggtgggc ggcgaaacat cgcgagcacc accttcattc
      661 cgacaccgag ctcgacgtcc attcgccgcg tcagacgggc ctcctctata gccatgtcgg
      721 gtggattttc gcccggcgaa acgacggggc cgatctcgtc aaagtcgccg atctcgcccg
      781 ctacccggaa ctgatgtggc tgcaccgttt tgaactggcg ccggcggtcg cgctggcgct
      841 ggcctgcttc gggttggccg ggtggccagg ccttgtcgtc ggcttctttt ggagcacggt
      901 cgcggtctat cacgcgacgt tctgcatcaa ttctctcgcc cattgcattg gccgcaagcg
      961 ctacctgacg ggcgatgatt cgcggaacaa ttggctgctc gcctttttca cgatgggcga
     1021 gggctggcac aacaatcatc atgctttcca aagcagcgtc cgccaaggct ttctctggtg
     1081 ggagtacgac gcgacgttct atcttctcaa gcttctcgcc aagctcggcc tgatttggcg
     1141 gctgaaactg ccgccggccg cggtgctgga gaaccggcat cgcgtcggcg cgcgtatcgt
     1201 gcagcgcgcc gcggcgcaag tcgctgacag tttcaacgcc gagcggatcg ccggcctcat
     1261 cgcggcgacg ctcggcaagg ccggattgtc gcgtctgcac gcccgcctgc tcgacgcgcg
     1321 cctgcgcatg gccgaggttc tctcggagat ggatttgccg gaactgccga gccggaccga
     1381 catcgtcaag cgcgcccgct cgatgatggc gatcaacgcg caatcgatcg aggagatcgg
     1441 cgcgcgcgcc catcaactgc tgctggaggc ggtcggctcg cgcctcgccg gcctcgccgc
     1501 gcaatcggcg gagtaggcgt cgacgagaga ggcttcggtc accggttcca atcgagccat
     1561 tgggcgcgct tggctcaagg aaacggcagg cccatccggc cgccgaggga ttggctgagg
     1621 ctcaacagct tggcgcgcgc gagatgggtt tgcgccgcca ggcggatcag cggcgccaga
     1681 tcggaaggat tgtcgagcaa agcccgcagc accgccgggg cgtcgatcga gccgtcgacg
     1741 cgtcgcccgg cgagagcggc gtcgggcaat ccgcaccgcg cgtcgtcggc gacgacccgc
     1801 acggcggcga gcggcagacg gtgagcgctc gccgccgcgg cggcgagatg agactccatg
     1861 tcgacgacgg ccgcgccgaa ccgttcgtag aggcggctct tctcggcgat gtgaatcacc
     1921 ggcgcgtcga cgccaaggat cggcgcgacg atcgcgccgg gaatggattg aaggagacgc
     1981 cgtgtccatt cctcgttggt gcgccaaatc ctggcgccgt catgaataga ggaggcgatg
     2041 atgcaggccc cggcgcgcag gcccggggca agtccgccgc atatgccgaa gctgatgatg
     2101 ccgcgattgg cggcgatgat gttggccgac agcaagttcg ggtccggccg gaggaagacg
     2161 gcccggatgg atcggctggc gagaatgcgc gcctcgaatt gcaggccgac aatggcgaga
     2221 atatccgagc tcggcggcgc tgggcgccat agcgacgctt ccctccctcc ttcggcgaag
     2281 aaatccggcc agcgccaggc cggctcccag tccggccaga tcttgccgtc gagataggag
     2341 cgcaggcggt ggctgatcgg ccaaagctcg cgccaattga aacgccgcga gtcggcgtcg
     2401 gcgacgagag acggctccgc ctcgccggcg gcgtaagcgt cgcgcgtcgc gcctgccgtg
     2461 gtctcttcgc cgatatgatt gccgatgaac acggcgctta cgacctctca gcctcttcga
     2521 tgcgccgccc cgccggcgcc agccttcaat atcgcctgcc agacgccgct ccgacgccgg
     2581 cgctaagccg gaggaaaccg gcgcggcggc ctgacatctc aagaccgccg aaaagccttc
     2641 atgccgaaga aggtgaaccc gcattcaact gaattctgca gcaaaacaat tccgggccca
     2701 ttcaggttcc gcatgatgac ctcgtatgaa aacgccgcat catccaggca ggtcgccata
     2761 gccgccaatg ctgcgcccgg cgcgtcgtaa agcctcgatg ctcagcggat cgacaagcgc
     2821 agccaattca tcgacgtagc ggtagccggg cgcgcatcca gggaatgcgt cccgcgtcgc
     2881 cggatgaaga tagatttccc aaaagccagg cggcaggcgc gacagtaagg attgaaggcg
     2941 ctgcgccgtc atgcagccgg accagcgcaa gcccagcacg ccgtcggcgg tggcgagccc
     3001 cgcgcgcttg gccaggcggc gcagccaagc ggcgcagccc tgctcgaccg cgcgataagc
     3061 aggcgcggag gactcgtcgg cgagcgcgat catgcggcgc ggctcatagg gcgcgcgcaa
     3121 ggcgcgcatg cggtaggcgc gaccgatccc gatcaccatc gccgccacga ccgggtgaag
     3181 gtggaagtgc tggtgaacat tgacgtgatc gagcgccaag cccgtcgcgc gaaaggccgc
     3241 gaattgcgcc tcgatctcgg cgcgcatctg gcggcgcgcg tcccggtcga cggcgagatc
     3301 gaaaccgagc cgcgcgaggt cggcgcgcag gcggccggcg cggtcgacga gttggggaat
     3361 tttctccggc ggcagagcgg ggagggcgtc ggtcaaagcg agatgcaggc ccacgcgtag
     3421 gttaggcatc gaacgggcgc gggagacggc gtcttgcgcc tcgggcgcgg cgaccatcag
     3481 gctggctgcc gacaaaatcc ccttccgatg cgcgatctcg acggcctcgt tgacctcgcg
     3541 cgcgaggccg aaatcgtccg ccgtgacgac gagacgattc agtgagccaa gctccggcgg
     3601 tcgcgaagga acgagaagaa ctcgacgcct tcgcg
//
LOCUS       DATGJW010000160         4804 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_236630, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000160 DATGJW010000000
VERSION     DATGJW010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4804)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4804)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4804
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_236630"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            135..515
                     /locus_tag="VKU03_04875"
     CDS             135..515
                     /locus_tag="VKU03_04875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70627.1"
                     /translation="MSEETFTVALGGRHWALPHLPFRAIKKIQPILFQLYAELGGAEA
                     SPQSLARFTEAQLDRLAEAAYLAVAAVDKELSREAFLDLPFSVGELMLAFPAIARAAG
                     LRPVAPEQAAAEAPRPLGEPISTA"
     gene            789..2111
                     /locus_tag="VKU03_04880"
     CDS             789..2111
                     /locus_tag="VKU03_04880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70628.1"
                     /translation="MSDANLEVRFSASIDDLTQGVGEVKDALADLAEAASRMNGQYRT
                     LGASILAAMSPDKLQAFNAALLSSASIEKSLAAAHDQAAKAIRDGDDASYADAVRAAK
                     LAMNEEVKAVEDGMRQKLALYAAEAQRHEITQQDKVALSRQAMDEEYQAELALLQKEM
                     ALSGQTLTQKQQIDDKILDAERRHQDQIAQLVQRSITEQQREYENYANAVTQAFNSQL
                     HGLLAGSTNWRTAYKNMLSDLLIKSIEWGEASVVQHVANEAAKTAATTAGVAARTGAE
                     QAGASASLAAQAATIIRSIFSSAAETFAGVFGFLSPIMGPLAAGPAAAAQATVAGMAG
                     AVASADIGMWRVPADMLTLVHHNELVMPAAQASAFRDMLSAAGGDNAAPRSGRAMHIH
                     PTTNFHVSAIDSGSVAQWIKANSASMAKAMDEAARHGALLGLRRLAGA"
     gene            2121..4607
                     /locus_tag="VKU03_04885"
     CDS             2121..4607
                     /locus_tag="VKU03_04885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177052.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase TIM-barrel-like
                     domain-containing protein"
                     /protein_id="HLJ70629.1"
                     /translation="MGFVGGVHLLPATGEFAYDTIPHQAAEAGGAMAGINTLHAPGGA
                     KTDYSYAIDQLQAAHPECGTVSILCAWFADSLDAASCRIYPSTNFIGGVFQQLAGSSW
                     TVDSWRVSGLTQSSSGLIPLPTSGGGAIYGGTPSDQSVVRCIQDLKARGFKVVFYPFL
                     LTTAAGLPWRGRIAYAPDLSSAATDAVDAFLGPAAREQFTPDAANLTVAYAGSPTDFT
                     YRRMILHYAWLCALAGGVNLFLLGSELRGLETIRGPSWTKAGTTDGSGYAVWDYPFVA
                     GLQALAADVRAIFDGQGLAKDAVNLDNLIAYSADWSAWMGYQHPGEGGQWPHLDQLFA
                     AGDVDLVGIDNYLPLTDWTTGEGGLDALNWSAPARSGPWPPASAASGGLGLSGPPALY
                     SLDYIKANIEGGEKYDWYYNDGVNGGRGLDPNGSDRQVSLPQGDRLIQARNAYSANQQ
                     LLANKQFRWWWSNPHQAVYDSGDGQGWAPHGPQTQWRACAKPLAFIEYGFPAADKATN
                     QPNVFFDSRSSESATPYWSLWAEAEGGGFLPQRDDTLMMLALQAVYEYWNGDGHNETS
                     PGGVKLIEFAFSCVWAWDARPFPTFPLLTSQWSDGANWFAGNWLSGRGPALPPLAASP
                     APTPGSYSSFPALVTLGWSTRVKPRFATQIADRASGRSSRRPLYEAALYDIELTYDLL
                     RGDEAHRELQEIAGFFAASGGRREPFWLAPPGLAEVFGQALGTGDGIKTIFPLVRWFG
                     TYVEPAAATSGVAAVYLDGVLAPSAQYSVTSGYGPSIVFAAPPPSGAAIAADFGLLWL
                     CRFSDDALDLDEFMAMLFELRLVKLQMVRP"
     gene            4604..>4804
                     /locus_tag="VKU03_04890"
     CDS             4604..>4804
                     /locus_tag="VKU03_04890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2460 domain-containing protein"
                     /protein_id="HLJ70630.1"
                     /translation="MTPPPSFPSLAGQGWSVHKKPVFSTLVAGHVSGREARDALWQYP
                     LWEFELCFDGLASDASSYPGLGA"
BASE COUNT          760 a         1601 c         1601 g          842 t
ORIGIN      
        1 ctgctctata attgcgtcgc cgagaaactc gccttcggca ccaagctcga ggatttcgtc
       61 ctgcccgagt tggacttcca gtgcttcgcc aacagcgccg gccaggtctg ccagctcaat
      121 ttcggagacg cggcgtgagc gaggagacgt ttacggtcgc gctcggcggc cggcattggg
      181 cgctgccgca tctgccgttc cgcgcgatca agaaaatcca gccgatcctg ttccagctct
      241 atgcggagct gggcggggcc gaggcttcgc cgcaaagcct agcgcgcttc accgaggcgc
      301 aactcgaccg gctcgccgag gcggcctatc tcgccgtcgc ggccgtcgac aaggagctaa
      361 gccgcgaggc gtttttggat ctgcccttct cggtcggcga attgatgctc gcctttccgg
      421 cgatcgcgcg cgccgcgggt ttgcggccgg tcgcgcccga acaggcggcg gcggaggcgc
      481 cgcgcccttt gggggagccg atttcgaccg cctgatcgcc catgtcgtcg ccaataccgg
      541 ctggacctgg gacgaggcgc tggacggctt gaccttgccg cgcctcgccg ctctgcaagc
      601 ggaatggcga gcccatccgc cggggcattg gctgctggcg gcgctcatga agtacaaacc
      661 tccgtccgag gcggatgtcg gcgcgcggca aacaagcgtg gcggcggttc aggcggcctt
      721 tccgggcgga aggttgtgaa gtcagtcgtc aattgacagc ggccgatgac gccgcccgcg
      781 ggggagccat gtccgacgcc aatctcgaag tccgcttctc cgcctcgatc gacgatctga
      841 cccagggcgt cggcgaggtg aaggacgcgc tcgccgatct tgccgaggcg gcgagccgga
      901 tgaacgggca ataccggacg ctcggcgcct cgatcctcgc ggcgatgtcg ccggacaagc
      961 tgcaggcttt caacgccgcg ctgctctcct cggcgtcgat cgaaaagtcg ttggccgcgg
     1021 cgcatgacca ggccgcgaaa gcgatccgcg acggcgacga cgcttcctat gccgacgcgg
     1081 tccgcgccgc aaagctcgcg atgaatgaag aggtgaaagc cgttgaagac ggcatgcggc
     1141 agaagctcgc gctctatgcc gccgaggctc agcgccacga aatcacgcaa caagacaaag
     1201 tcgccctctc gcgccaggcg atggacgagg aatatcaggc cgagctcgcg ctgctgcaga
     1261 aggaaatggc gctgagcggg cagacgttga cccagaagca gcagatcgac gacaaaattc
     1321 tcgacgccga gcggcgtcat caggaccaga tcgcgcagct cgtgcagcgc tcgatcacag
     1381 agcagcagcg cgaatacgaa aactacgcca acgcggtcac gcaggccttc aattcgcaat
     1441 tgcacggtct gctcgccggc tcgaccaatt ggcggaccgc gtacaagaac atgctcagcg
     1501 accttttgat caagtccatc gaatggggcg aggcgagcgt cgtccagcat gtcgccaacg
     1561 aggcggcgaa gaccgcggcg accacggcgg gcgtggcggc gcgaaccggc gccgagcagg
     1621 cgggcgcgtc ggcctcgctc gccgcgcagg ccgcgacgat catccgctcg atcttctcct
     1681 cggccgccga gaccttcgcc ggcgtgttcg ggtttttgtc gccgatcatg gggccgctcg
     1741 ccgccgggcc tgccgccgcg gcgcaagcga cggtcgccgg catggccggc gcggtggcgt
     1801 cggcggatat cggcatgtgg cgggttccgg ccgacatgct gaccctggtc catcacaacg
     1861 aactggtgat gccggcggcg caggcttccg cttttcgcga catgctgagc gcggccggcg
     1921 gcgataacgc cgcgccgcgc tccggccgcg cgatgcatat ccatccgacg accaatttcc
     1981 acgtctcggc gatcgattcg ggctcggtcg cgcaatggat caaggccaac agcgcgtcca
     2041 tggccaaggc gatggacgag gcggcgcggc acggcgcctt gctcggcttg cggcggctcg
     2101 ccggcgcgtg aggcttgacg atgggcttcg tcggcggcgt gcatctcctg cccgcgacgg
     2161 gcgagttcgc ctatgacacg atcccgcatc aggccgctga agcgggcggc gccatggccg
     2221 gaatcaacac gctccatgcg cccggcggcg ccaaaacgga ctattcctac gccatcgatc
     2281 aattgcaggc ggcgcatccg gaatgcggga ctgtgtcgat tctttgcgcc tggttcgccg
     2341 actcgctcga cgccgcaagc tgtcgcatct atccttcgac caatttcatc ggcggcgttt
     2401 tccagcagct tgccggctcg tcctggaccg tcgattcctg gcgcgtctct ggtctgactc
     2461 aaagctcgtc cggcctgatc ccgctgccga ccagcggcgg cggcgcgatc tatggcggca
     2521 cgccgtccga ccagagcgtg gtgcgctgta tccaggacct caaagccaga ggcttcaaag
     2581 tcgttttcta cccgtttctt ttgacgacgg cggcgggact gccctggcgc ggccggatcg
     2641 cctatgcgcc cgatctgagc agcgccgcga ccgacgcggt cgacgccttc cttggcccgg
     2701 cggcgcgcga gcaattcacg ccggacgcgg ccaatctcac tgtcgcttac gccggctcgc
     2761 cgacggattt cacctatcgg cggatgatcc tccactacgc ctggctctgc gcgctggccg
     2821 gcggcgtcaa cctcttcctg ctcggttcgg aattgcgcgg gctcgagacg atccgaggcc
     2881 cgtcatggac caaggccggg acgaccgacg ggtcgggcta tgcggtctgg gattatccgt
     2941 tcgtcgccgg attgcaggct ctggccgccg acgtgcgggc gatcttcgat gggcagggct
     3001 tggccaagga cgcggtcaat ctcgacaatc tcatcgccta ttccgccgac tggtcggctt
     3061 ggatgggcta tcagcatccg ggcgagggcg ggcaatggcc gcatctcgac caactcttcg
     3121 ccgcgggcga tgtcgatctt gtcggcatcg acaattacct gccgctgacg gactggacga
     3181 cgggcgaagg cgggctggac gcgctcaact ggagcgcgcc ggcgcggtcc gggccatggc
     3241 cgccggccag tgcggcgagc ggcgggctcg gcctttccgg gccgccggcc ctttacagcc
     3301 tcgactatat caaggccaat atcgagggcg gcgagaagta cgattggtac tacaatgacg
     3361 gcgttaatgg cggccgcggc ctcgatccga atggatcgga ccggcaggtc tcgctgccgc
     3421 agggcgatcg gctgattcaa gcgcgcaacg cctattcggc caaccagcaa ttgctcgcca
     3481 acaaacagtt ccgctggtgg tggagcaatc cgcatcaggc ggtctacgat tccggcgacg
     3541 gacagggctg ggcgccgcac gggccgcaga cgcaatggcg ggcctgcgcc aagccgctcg
     3601 ccttcatcga atacggcttt cccgccgccg acaaggcgac caaccagccc aacgtattct
     3661 tcgattcgcg ttccagcgag agcgcgacgc cctactggtc gttatgggcg gaggcggaag
     3721 gcggcggctt cctgccgcag cgcgacgaca cgctgatgat gctggcgctg caggcggtct
     3781 atgaatattg gaacggcgat ggccataacg agacctcgcc cggcggcgtc aagctgatcg
     3841 agttcgcctt ctcatgcgtt tgggcgtggg acgcgcggcc cttcccgact tttccgctct
     3901 tgaccagtca atggtcggat ggcgccaact ggttcgcggg aaattggctt tcggggcgcg
     3961 ggccggcgct gccgcctctc gccgcctcgc cggcgccgac gcccggctcg tattcaagct
     4021 ttccggcgtt ggtcacgctc ggctggtcga cccgcgtcaa gccgcggttc gcgacgcaga
     4081 tcgccgaccg cgcttccggc cgctccagcc gccggccgct ctatgaggcg gcgctttacg
     4141 acatcgagct gacctacgat cttctgcgcg gcgatgaggc gcatcgcgaa ttgcaggaga
     4201 tcgccggctt tttcgccgcg agcggcgggc ggcgcgagcc gttctggctc gcgcccccgg
     4261 ggttagccga ggtcttcggc caggcgctgg gaaccggaga cgggattaag accatctttc
     4321 cactggtacg gtggttcggg acatatgtcg aaccggccgc cgcgacctcc ggcgtcgccg
     4381 ccgtttatct cgacggcgtc ctcgcgcctt ctgcgcaata ttccgtgacg agcggctatg
     4441 gcccgtcgat cgtcttcgcg gcgccgccgc cttccggcgc tgcgatcgcg gcggatttcg
     4501 gcctgctctg gctgtgccgc ttctcggacg acgcgctcga tctcgacgag ttcatggcga
     4561 tgcttttcga attgcgcctg gtgaaattgc aaatggtgag gccatgacgc cgccgcccag
     4621 ctttcccagc cttgccggcc aaggctggtc ggtgcacaag aagccggtct tttcgacgct
     4681 ggtcgccggc catgtctccg gccgcgaggc gcgcgacgcc ttgtggcaat atccgctttg
     4741 ggagttcgag ctctgcttcg acgggctcgc ctcggacgcg tcgagctatc caggtctcgg
     4801 agcg
//
LOCUS       DATGJW010000161        10374 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_755205, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000161 DATGJW010000000
VERSION     DATGJW010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10374)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10374)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10374
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_755205"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..641)
                     /locus_tag="VKU03_04895"
     CDS             complement(<1..641)
                     /locus_tag="VKU03_04895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLJ70631.1"
                     /translation="MRAKVSTFGVHAVLTAYTLLAVAPILLVIMNSFKSRAAIFGAPL
                     TPPSAATFSLIGYNKALGAAHVGWFFFNSITVTVVSMGLVLLLGAMAAWALTEYRFRG
                     STWLALFLSIGVMVPIRLGSIAILTMMRNAHLNDTLIALILVYIAQGLPLAIFILSEF
                     IEQIPKDLRDAARCDGVPETRIFFEVIAPLLRPAIATVAVFTIVPIWNDLWFPL"
     gene            complement(874..1824)
                     /locus_tag="VKU03_04900"
     CDS             complement(874..1824)
                     /locus_tag="VKU03_04900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLJ70632.1"
                     /translation="MAQARSQVVAYMPRSRFVGLMLLFLGPAVAIYTAISIYPLLATM
                     ALSTYATTETGEHIFIGLDNFRTLFTDAVWSKPFWNACLNNLIFFLVHMAVQNPIGII
                     LAGLLSLPNLKLKATYRTLIFMPTMLSVVIIGFSWSLILSPLWGVAEGALKFIGLGSL
                     FRPWLGLPSTALVTLALISVWQFVGIPMMLIYAALLNIPDELIDAARVDGLGHLRIFA
                     HIKLPLVLPTIGLVSILTFVGNFNAFDLIYSVKGALAGPNFAADILGTFFYRTFFGYQ
                     LQAGSPTMGAAIATVMFFIILAGVCVYLFGVQRRLQRYTF"
     gene            complement(2070..3332)
                     /locus_tag="VKU03_04905"
     CDS             complement(2070..3332)
                     /locus_tag="VKU03_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ70633.1"
                     /translation="MNRLLSNGLKAGAALAALLVGITIAEAGNLKIESWRNDDADIWN
                     SKIIPAFNKHYPDIKVEFSPTAPKEYNAALNARLAGGTAGDLITCRPFDASLELYNKK
                     QIDGLNDLKGMSDFSSVAKSAWTTDDGKTTFCVPMASVIHGFMYNKDAFRKIGAEEPK
                     TMAEFHAILDKLKKDGTYTPIVLGTNDQWEAATMGFQNIGPNYWKGEDGRLALIAGKE
                     KFTDPQYVAVFKELATWGPYMGNGYQAQSYPDSQNLFSLGKGAIYPAGSWDISTFRAQ
                     AKFPIGVFKPPLPEGAKDCYISDHTDIAMGVNSASKNKEDAKKFLEWLATPEFATLYA
                     NALPGFFPLSSAPVKIDDDLAATMVGWRKECKSTIRNSYQILSRGTPNLENELWNVSA
                     QVINGAMTPEAAAKQVQDGLDKWYKPAK"
     gene            complement(3374..4261)
                     /locus_tag="VKU03_04910"
     CDS             complement(3374..4261)
                     /locus_tag="VKU03_04910"
                     /EC_number="4.2.1.126"
                     /inference="COORDINATES: protein motif:HMM:NF003915.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramic acid 6-phosphate etherase"
                     /protein_id="HLJ70634.1"
                     /translation="MAAQDESDSHGETGFIAALIAGQRRAVDAVATALPSIERAAAAI
                     ADKLGVGGRLIYLGAGSSGLIAMLDGAELPGTFGLDSSRIRFVLPGGVGRAYQIDGAT
                     EDDREAGRRDIEALGDIAGDIVIAVSASGSTPYTLAGARAAAARGATIVALANRPGSP
                     LLQNAHHPILLDSGPEALQGSTRLGAGTAQKCALGLLSTLANARLGHVYRGLMVNVRP
                     DNDKLRRRALEIVAAIADVDEPAARRSLACAADDVKCAALIAAGAGSKERADDLLARS
                     RGHVGAALSRLREHAASRA"
     gene            4407..5315
                     /locus_tag="VKU03_04915"
     CDS             4407..5315
                     /locus_tag="VKU03_04915"
                     /inference="COORDINATES: protein motif:HMM:NF013986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BadF/BadG/BcrA/BcrD ATPase family protein"
                     /protein_id="HLJ70635.1"
                     /translation="MSETLQSDFFLGVDGGATRCRARLRDAAGQALADVEGPAANIYV
                     DFERGLAVVRETTAQALARGGLAAADRARVALGVGLAGLSSRQEAARVAAALPGWRAV
                     SAVNDAIAACLGAHGGADGGLIIAGTGSAGAARVEGRDTIIGGRGFLLGDDGAAARIG
                     ADALRAALRAHDGLQIASPLTQSVMRHFSDDPLAMLNWALTAKPSDYGAFAPQTLAAA
                     AAGDAVAIPIVEAAAAALGALAAALEKLGAPRLVMVGGLAGPIRPYLPPSLAAKLRPP
                     LFDAVDGAILAAGGVPPKQKEEGRRA"
     gene            5312..6064
                     /locus_tag="VKU03_04920"
     CDS             5312..6064
                     /locus_tag="VKU03_04920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ70636.1"
                     /translation="MTIAEAPPRATLYTTVGETLKREIASGRFADTEVLPGERELSAL
                     LSVSRTTLRRAITDLVAEGVLYHRHGAGTFIRRAVPRVDQALSRLTGFTQDMRLRGFI
                     AGSRELERGAFLPTPEEAMMLGVSPNEGVIRLSRLRLATDVPMAIEHAVIPQRYIPDA
                     AMIGSSLYDTLEARDFKPVRALQRLRAVLLGEADARLLGVEPKSPALYIQRIAYLADG
                     RCVEFTRSYYRSDTYDFVSELTLAPAARRSKA"
     gene            6061..7101
                     /locus_tag="VKU03_04925"
     CDS             6061..7101
                     /locus_tag="VKU03_04925"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008597265.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HLJ70637.1"
                     /translation="MSAKQTPSLMIREIAESGDALARQWAANAEQTKRYAASIRRRDP
                     RVVATIARGSSDHCALYLKYLLEILLGVPCASIGPSIASLYNAELKLEGALALAISQS
                     GRSPDIVAMQDAAKRGGAMTLALVNDVSSPLAKGADCLLPLHAGPERSVAATKSMIAT
                     LFAGAALLAEWRQDAALSAALAKLPALLRAQSEPPPDPLVARVAAAKSAFVLGRGATF
                     AIAAEAALKLKETCAIHAEAFSSAEVLHGPAGIVAAGFLVIAFLPQDEARDGMSDTLR
                     RLAAMGADILAVDALDTDGPMALAAAVADHPLLTPIAMIHRFYRLAEACARRLGRDPD
                     NPPHLRKVTETR"
     gene            7101..8243
                     /gene="nagA"
                     /locus_tag="VKU03_04930"
     CDS             7101..8243
                     /gene="nagA"
                     /locus_tag="VKU03_04930"
                     /EC_number="3.5.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018093832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008448 - N-acetylglucosamine-6-phosphate
                     deacetylase activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylglucosamine-6-phosphate deacetylase"
                     /protein_id="HLJ70638.1"
                     /translation="MLVFTGARLFDGEGFLDGHALVVENRTIVALAPHAERPRGGEER
                     DLGGGVLAPGFIDWQVNGGGGVLFNEAPTIEGIRAIVEAHRRYGTTALLPTVITDAPG
                     TLAAALDAARGAEREVSGALGVHVEGPFIDPRRKGAHPPEWIRPMRESDAAALIAARS
                     GIMVVTLAPASASYERIAQLAKSGVVVSLGHSDASAEEARGAFDAGATAATHLFNAMS
                     QLGHRAPGVVGAALSDPRVVCGFIADGYHVHEAATRVAFKAKGARGIALVSDAMPPAA
                     GGPHIYELNGRRVTQRGLKLTLDDGTLAGAAITMLDAVRYVRRTIGIDLADALTMATA
                     TPARLLKVDDRYGRLKPGFRADLVHLDDDLSLKGVWVGGVESFRPA"
     gene            complement(8266..8988)
                     /locus_tag="VKU03_04935"
     CDS             complement(8266..8988)
                     /locus_tag="VKU03_04935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70639.1"
                     /translation="MSAALIAEGLHTYYGKSHILRGVGLEVAEGKITALLGRNGAGKT
                     TTLRSLIGLTPPRAGKVVIFGEDVTHRPAFRIACLGVGYVPEGRRIFPNLSVEENLKV
                     PIERDGPWTIPRIYGLFPRLAERRRNRGRQLSGGEQEMLAIARALLLNPKLLLLDEPS
                     QGLAPLIVRDVFRVVSAMREEGLSVLLVEQNAHMTLAIADYAYVIDDGAIVHAGPARE
                     LAQDAARVQALAGVAAEAWGEF"
     gene            complement(8985..9782)
                     /locus_tag="VKU03_04940"
     CDS             complement(8985..9782)
                     /locus_tag="VKU03_04940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70640.1"
                     /translation="MSAEGGASATEEAEPSGAILKVESVSKRFGDLIAVRDVSLVVPR
                     GELRAIIGPNGAGKTTFFNLLSGFFPPTSGRILFDGRDVTTVAAHRRVELGMARTFQI
                     TEIFPELTVHENLRIGAEIAAGYALRPWISRAERSSVQRKVEEILDIAGLAAKASRLV
                     GQLAHGDQRVVEIATALTLQPRLLLLDEPTAGMGDQETYAVSKLVRALHRRGDLSIVL
                     IEHDMRVVFNLAERITVLDQGLLLAEGAPAEIAANAAVQAAYLGEAA"
     gene            complement(9779..>10374)
                     /locus_tag="VKU03_04945"
     CDS             complement(9779..>10374)
                     /locus_tag="VKU03_04945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002716559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70641.1"
                     /translation="FGQVLYYIAFQWSSLTGGDDGLRGFARQPLDLGFARIDILSNET
                     AFYYFVLLCFALLVAVMGFILRSPFGRAMIAIRENERRARFLGLAVERHIWIAFTLSC
                     LFMGFAGALYALVNNFADPRGLHYSQSGDFVMMAVMGGMRNFWGPLLGAVVFVVVQDY
                     LSSVTVNWMSFVGLLFVLVVLFFPRGLLGFVGKRAAA"
BASE COUNT         1793 a         3360 c         3488 g         1733 t
ORIGIN      
        1 agcgggaacc agagatcgtt ccagatcggc acgatggtga agacggcgac ggtggcgatg
       61 gccgggcgca acagcggcgc gatcacttcg aagaagatgc gcgtttccgg cacgccgtcg
      121 cagcgcgcgg cgtcgcgcaa gtccttgggg atctgctcga tgaattcgga caggatgaag
      181 atcgccagcg gcaggccttg cgcgatatag acgaggatca gcgcgatcag cgtgtcattg
      241 agatgagcgt tgcgcatcat ggtcaggatg gcgatcgagc cgaggcggat cggcaccatc
      301 acgccgatcg acaggaacaa agcgagccag gtcgagccgc ggaaccgata ttcggtcagc
      361 gcccatgccg ccatggcgcc gagcaacagc acgaggccca tgctgacgac cgtgactgtg
      421 atcgagttga agaaaaacca gccgacatga gccgcgccga gcgccttgtt atagccgatc
      481 agggaaaagg tcgcggccga tggcggcgtc agcggcgcgc caaaaatcgc cgcgcggctc
      541 ttgaacgaat tcatgatgac gagaaggatc ggcgcgacgg cgagcagcgt ataggcggtc
      601 aacaccgcgt ggacgccgaa ggtggagact ttcgcgcgca tggctagtgt ccgtactcgc
      661 tgcgcaaata accgatacag gggcgtaccc tcatccggcc gcttcgcggc caccttctcc
      721 cgcaagcggg agaagggttg ttgccgcggt cggcgaccgc ggctacagcg cggcggctga
      781 tgaagcgtgc ggcgcgcccc ctctcccgct tgcgggagag ggctggggtg agggcaagcc
      841 gcagtgttcg tcggtcggtc acgacaaacc cgatcaaaac gtatagcgct gcagccggcg
      901 ctgcacgccg aacagataga cgcagacgcc ggccaggatg atgaaaaaca tgacggtggc
      961 gatcgccgcg cccattgtcg ggctgccggc ctggagctga tagccgaaga aggtgcggta
     1021 gaagaacgtg ccgagaatat cggccgcgaa attggggcca gcgagcgcgc ccttgaccga
     1081 atagatgaga tcgaacgcgt tgaaattgcc gacaaaggtc aggatcgaga cgagaccgat
     1141 ggtcggcaag acgagcggca gcttgatatg agcgaagata cgcagatggc cgagcccgtc
     1201 gacccgcgcc gcgtcgatca gctcgtcggg aatgttgagc agcgcggcat agatcagcat
     1261 catcggaatg ccgacgaact gccagaccga gatcaaagcg agcgtgacaa gcgcggtcga
     1321 aggcaggccg agccatggcc tgaatagcga gccgaggcca atgaatttca gcgcgccttc
     1381 ggcgacgccc cagagcggcg agaggatcag gctccaggaa aagccgatga tcacgaccga
     1441 gagcatggtc ggcatgaaga tcagcgttcg atacgtcgcc ttgagcttca gattgggcaa
     1501 gctgagcaga ccggcgagga tgatcccgat cgggttctgc acggccatat ggaccaggaa
     1561 aaagatcaga ttgttgaggc aggcgttcca gaacggcttc gaccagaccg cgtcggtgaa
     1621 cagcgtcctg aaattatcga ggccgataaa gatatgctcg ccggtctcgg tcgtggcgta
     1681 agtcgacagc gccatcgtcg cgaggagtgg atagatcgag atcgccgtgt agatggcgac
     1741 cgcgggccct aagaacagca gcatcagccc gacaaaacgg ctgcgcggca tataggcgac
     1801 gacttgcgac cgtgcttgcg ccatggattt ctcgcgccgt cggcgtccct agtcttctct
     1861 gcttagaatg cccagaaccc tcatccggcc gcttcgcggc cgccttctcc cttaaagcgg
     1921 gagaagggat tttgtagccg cgatcggcga ccgcggcgcc agcgcggcgg ccgatgaaag
     1981 cgcctggcgc gtccctctcc cgctttaagg gagagggtga gcgccgagcg ctggcgcatc
     2041 gaatgcggcg cgccgtacct aagctggcct tacttcgccg gcttgtacca cttgtcgagc
     2101 ccgtcctgga cctgcttggc ggcggcctcc ggcgtcatcg cgccgttgat cacctgagcc
     2161 gagacgttcc agagctcgtt ttcgagattc ggcgtgccgc gcgacaggat ttggtaggag
     2221 ttgcggatcg tcgacttgca ctccttgcgc cagccgacca tcgtcgccgc cagatcgtcg
     2281 tcaatcttca ccggcgccga ggagagcggg aagaagcccg gcagcgcgtt ggcgtagagc
     2341 gtcgcgaatt ccggcgtcgc cagccattcg aggaatttct tggcgtcctc cttgttcttc
     2401 gacgccgagt tcactcccat ggcgatgtcg gtatggtcgg aaatgtagca atccttggcg
     2461 ccttccggca gcggcggctt gaacacgccg atcgggaact tcgcctgggc gcggaaagtc
     2521 gagatatccc acgagccggc cggatagatc gcgcccttgc cgagcgagaa caggttttgg
     2581 ctgtccggat aggactgcgc ctggtagccg ttgcccatat agggccccca agtagcgagc
     2641 tccttgaaca cggcgacata ttgcgggtcg gtgaacttct ccttgccggc gatcagagcg
     2701 aggcggccgt cctcgccttt ccagtaattg gggccgatat tctggaagcc catcgtcgcg
     2761 gcttcccact gatcgttggt gccgagcacg atcggcgtat aggtcccgtc ctttttcagc
     2821 ttgtcgagaa tggcgtggaa ctccgccatc gtcttcggct cttccgcgcc gatcttcctg
     2881 aaggcgtcct tgttgtacat gaagccgtga atgaccgagg ccatcggaac gcagaaggtc
     2941 gtcttgccgt cgtcggtggt ccaagcggat ttggcgaccg acgagaagtc ggacatgcct
     3001 ttcaggtcgt taagcccgtc gatctgcttc ttgttgtaga gttcgagcga ggcgtcgaac
     3061 ggccggcagg tgatgaggtc gcccgccgtg ccgccggcga ggcgggcgtt aagcgcggcg
     3121 ttgtactctt tcggcgcggt cggcgagaat tcgaccttga tgtcgggata atgcttgttg
     3181 aaagccggaa tgattttcga gttccagatg tcggcgtcgt cattgcgcca gctctcgatc
     3241 ttcagattcc ccgcctcggc gattgtgatc ccgacgagca gcgccgccag ggcggctccc
     3301 gctttcaaac cattgctgag cagtctgttc atttccgtct ccctcttcac gggcttcgcc
     3361 cgttgtttaa aatctacgcg cggctcgccg catgctccct gaggcgcgac aacgcggcgc
     3421 cgacatggcc gcgcgagcgc gcgagaagat cgtcggcgcg ctctttcgag cctgccccgg
     3481 cggcgatcag cgccgcgcat ttcacgtcgt ccgccgcgca ggcgaggctg cgccgcgcgg
     3541 ccggctcgtc gacgtcggcg atcgccgcga caatctcgag cgcccgtcgg cgaagcttgt
     3601 cattgtcggg acgcacgttg accatcaggc cgcgatagac atggccgagc cgcgcattgg
     3661 cgagagtcga aagcaggccg agcgcgcatt tttgcgcggt tccggcgccg aggcgggtcg
     3721 agccttgcag cgcctcgggg ccgctatcga gaagaatcgg atgatgagcg ttttgcagca
     3781 gcggcgagcc gggccggttc gcgagcgcga cgattgtcgc gcctcgcgcg gccgccgccc
     3841 gcgcgcccgc gagcgtatac ggcgtcgagc cgctcgccga gacggcgatg acgatgtcgc
     3901 ccgcaatatc gccgagcgct tcgatgtcgc gccgaccggc ctcgcgatcg tcctcggtcg
     3961 cgccgtcgat ctgatacgcc cggccgaccc cgccgggaag gacgaaccga atgcgcgaag
     4021 aatcaagacc gaacgtgccc ggcaattccg cgccgtccaa catcgcgatg aggcccgacg
     4081 atccggcgcc gagatagatc agcctgccgc cgacgcccag cttgtcggcg attgcggcgg
     4141 cggcgcgctc gatcgacgga agcgcggtcg ccacggcgtc gacggcgcgg cgttgcccgg
     4201 cgatcagcgc ggcgatgaag ccggtttcgc cgtgactgtc gctctcgtct tgcgcggcca
     4261 tccggcctcc ctgatcgccc cttgagccgg cgcgcgccga gccaaagaac caattttaga
     4321 accagtggtc cctacctctt gtcttaactg ttaacggaat gtatacctct tgtctagagc
     4381 cgattcacgg ctggcgcgga cggaatttga gcgagactct gcagagcgat ttctttctcg
     4441 gcgtcgacgg cggcgcgacg cgctgccgcg cacgtctgcg cgacgccgcc ggccaggcgc
     4501 tcgccgatgt cgaagggccg gcggccaata tctatgtcga tttcgagcgc ggcctcgcgg
     4561 tggtgcgcga gacgacggcg caggcgctgg cgcgaggcgg tcttgcggcg gcggatcggg
     4621 cgcgggtcgc gctcggcgtc gggctggccg gcctttccag ccggcaggaa gccgcgcgcg
     4681 tcgccgccgc tttgccggga tggcgcgcgg tgagcgcggt caatgacgcg atcgccgcct
     4741 gcctcggcgc gcatggcggc gccgatggcg gcctcatcat cgccgggacc ggctcggcgg
     4801 gcgccgcgcg ggtggagggg cgcgacacga tcattggcgg gcgcggcttt ctcctcggcg
     4861 acgacggcgc cgcggcgcgg atcggcgccg atgcgctgcg cgcggctttg cgcgcccatg
     4921 atgggctgca aatcgcctcg cctttgaccc aaagcgtgat gcgccatttc tcagacgatc
     4981 cgttggcgat gctgaattgg gcgttgaccg ccaagccgag cgattacggc gccttcgcgc
     5041 cgcaaacgct cgccgccgcg gcggccggcg acgcggtcgc aattccgatc gtcgaagccg
     5101 cggcggccgc gcttggcgcc ttggccgcgg cgctggaaaa gctcggcgcg ccgcgcctcg
     5161 tgatggtcgg cggccttgcc gggccgatcc gaccgtatct tccgccatcg ctggccgcga
     5221 aattgcggcc gccgcttttc gatgcggtcg acggagccat cctcgccgcc ggcggcgtgc
     5281 cgccgaaaca aaaagaggag ggtcggcgcg catgacgatc gccgaagccc cgccgcgcgc
     5341 gacgctctat acgacggtcg gcgagacgct gaagcgcgag atcgccagcg gccgtttcgc
     5401 cgataccgag gtgctgcccg gcgagcgcga gctttccgcc ctgctcagcg tctcgcgcac
     5461 caccttgcgc cgcgcgatca ccgatctcgt cgccgagggc gtgctctacc atcgccatgg
     5521 cgccggcacg ttcatccgcc gcgccgtgcc gcgcgtcgac caggctctgt cgcggctgac
     5581 cggcttcacg caggacatgc ggttgcgcgg ctttatcgcc ggctcgcgcg aattggagcg
     5641 cggcgctttc ctgccgacgc cggaagaggc gatgatgctc ggcgtcagtc ccaatgaggg
     5701 cgtgatccgc ttgtcccgct tgcgtcttgc gaccgacgtc ccgatggcga tcgagcacgc
     5761 ggtcattccg cagcgctaca ttccggacgc ggcgatgatc ggctcttcgc tttacgacac
     5821 gctggaggcg cgcgatttca agccggtccg cgccttgcag cgcctgcgcg cggtcctgct
     5881 cggcgaggcg gacgcgcgat tgctcggcgt cgagccgaaa agtccggcgc tctacatcca
     5941 gcgcatcgcc tatcttgccg acgggcgctg tgtcgagttc acgcgctcgt actatcgctc
     6001 cgacacctat gatttcgtgt ccgagctgac tctcgcgccg gcggcccgca ggagcaaagc
     6061 gtgagcgcga agcaaacgcc atcgctgatg attcgcgaga tcgccgaatc cggcgacgcg
     6121 cttgcccggc aatgggcggc gaacgccgag caaaccaagc gctatgcggc gtcgattcgg
     6181 cgccgcgatc cgcgggtcgt cgcgaccatc gcgcgcggca gctccgacca ttgcgcgctt
     6241 tatctgaaat accttcttga aatccttctc ggcgtccctt gcgcgtcgat agggccgtcg
     6301 atcgcctcgc tttacaacgc cgaactcaag cttgagggcg cgctggcgct ggccatttct
     6361 caatccggcc gcagcccgga tattgtcgcg atgcaggacg cggcgaagcg cggcggcgcg
     6421 atgacgctgg ccttggtcaa cgacgtctcg tcgcctttgg ccaaaggcgc agactgtctg
     6481 ctgccgctgc atgccggccc ggagcgatcg gtcgccgcca ccaagtcgat gatcgccact
     6541 ctcttcgccg gcgcggcgct gctcgccgaa tggcgccagg acgcggctct aagcgccgcg
     6601 ctggccaagc ttccggcgct gctgcgcgcc cagagcgagc ctccgccgga tccgctcgtc
     6661 gcgcgggtcg cggccgccaa atcggccttt gtgctcgggc gcggcgcgac cttcgccatc
     6721 gccgccgagg cggctttgaa gctcaaagag acctgcgcga tccacgccga ggcgttttcc
     6781 tccgccgagg ttttgcatgg ccccgccgga atcgtcgcgg cgggatttct ggtcatcgcc
     6841 ttcctgccgc aggacgaagc gcgcgacggg atgagcgaca ccttgcggcg gctcgccgcg
     6901 atgggcgccg acatcctcgc ggtcgacgct ctggacactg acgggccgat ggctcttgcg
     6961 gcggccgtcg ccgaccatcc tctgctcacg ccgatcgcca tgatccatcg cttttaccgc
     7021 ctcgccgagg cttgcgcgcg ccggctcggc cgcgacccag acaatccgcc ccatttgcgt
     7081 aaagtcacgg agacgcgctg atgcttgttt tcaccggcgc gcgtctcttc gacggcgagg
     7141 gatttctcga cggccatgcg ctcgtcgtcg aaaatcggac gatcgtcgcg ctggcgccgc
     7201 atgccgagag gcctcgcggc ggcgaagagc gcgaccttgg cggcggcgtc ctcgcgccgg
     7261 gtttcatcga ttggcaggtc aatggcggcg gcggcgttct cttcaatgag gcgccgacca
     7321 tcgagggaat ccgggccatc gtcgaggcgc atcgccgcta tggcacgacg gcgcttctgc
     7381 cgaccgtcat caccgacgcg ccggggacgc tcgcagcggc gctggacgcg gcgcgaggcg
     7441 cggagcggga ggtttctggc gcgctcggcg ttcatgtcga agggcctttc atcgatccgc
     7501 gccgcaaggg cgcgcatccg ccggaatgga ttcgcccgat gcgggagagc gacgcggcgg
     7561 ctttgatcgc cgcgcgaagc gggattatgg tcgtcacgct ggcgccggcc tccgcctcct
     7621 atgagcgcat cgcgcaattg gcgaaaagcg gcgtcgtcgt gagtctcggc cattccgacg
     7681 ccagcgccga ggaggcgcgc ggcgcgttcg acgccggcgc gaccgccgcc actcacctct
     7741 tcaacgcgat gagccaactc ggccatcgcg cgcctggcgt tgtcggcgcg gcgctttccg
     7801 atccgcgtgt tgtctgcggc ttcatcgccg acggctatca cgtccatgag gcggcgacgc
     7861 gcgtcgcctt caaggcgaaa ggcgcacgcg gaatcgcgct tgtctcggac gcgatgccgc
     7921 cggcggcggg cggcccgcat atctacgagc tgaacggccg acgggtcacc caacgagggc
     7981 tgaagcttac gcttgacgac ggcacgctgg ccggcgcggc gatcaccatg ctcgacgccg
     8041 tgcgctacgt ccgccggacg atcgggatcg atctcgccga cgcgctgacc atggcgacgg
     8101 cgacgccggc gcgcctgctg aaggtcgacg accggtacgg ccggctgaag ccgggcttcc
     8161 gcgccgacct cgtccatctc gacgacgatc tgagcctgaa aggcgtatgg gtcggcggcg
     8221 tcgaatcctt tcgccctgcg tagcgggaag aagggatgta cccggctaga actcgcccca
     8281 agcctccgct gcgacgccgg caagcgcctg aacgcgcgct gcgtcttggg cgagttcgcg
     8341 cgccggcccg gcatggacga tcgcgccgtc gtcgatgaca taggcgtaat cggcgatcgc
     8401 caaggtcatg tgcgcgttct gctcgacgag tagaaccgat aaaccctctt cgcgcatggc
     8461 cgagacgacg cgaaacacat ccctcacgat gagcggcgcg aggccttgcg acggttcgtc
     8521 gagaagcagc agcttgggat tgagcagcaa agcgcgggcg atggcgagca tttcctgctc
     8581 gccgcccgaa agctgccggc cgcgattgcg gcggcgctcg gcaaggcgcg ggaacagccc
     8641 gtagatgcgc gggatcgtcc atgggccgtc gcgctcgatc ggcactttca gattttcctc
     8701 gacgctgaga ttcgggaaga tgcgccgtcc ttccggcaca tagccgacgc ccaagcaggc
     8761 gatgcgaaaa gccggccgat gcgtcacgtc ctcgccgaat atgacgacct tgccggcgcg
     8821 cggcggcgtc aagccaatca ggctgcgcag agttgtcgtc ttgccggcgc cgttgcggcc
     8881 gagcagagcg gtgatcttgc cctcggcgac ctccagcccg acgccccgca ggatatgact
     8941 cttgccataa taggtatgca ggccttcggc gatcagcgcc gcgctcatgc cgcctccccg
     9001 agataggccg cctgcacggc ggcgttggcg gcaatctcgg ccggcgcgcc ttcggcgaga
     9061 agcaggccct ggtcgagaac cgtgatcctc tcggcgagat tgaagacgac gcgcatgtcg
     9121 tgctcgatga ggacgatgga aagatcgccg cgccgatgaa gcgcgcggac gagcttgctc
     9181 accgcgtagg tctcttgatc gcccatgccg gcggtcggct cgtcaagcag gagcaggcgc
     9241 ggctgcagcg tcagcgcggt ggcgatttcc acgactcgct gatcgccatg ggcgagctgg
     9301 ccgaccaggc ggctcgcctt ggccgccaag ccggcaatgt cgagaatttc ttcgaccttg
     9361 cgctgaaccg acgatcgctc ggcgcggctg atccaggggc gcagcgcata gccggcggcg
     9421 atttccgcgc cgatgcgcaa attctcatgg accgtcagtt ccgggaagat ttccgtgatc
     9481 tggaaggtcc gcgccatgcc gagctcgacg cggcgatggg cggcgaccgt ggtcacatcg
     9541 cggccgtcga acaggatgcg gccgctggtc ggcgggaaaa agccgctgag caaattgaag
     9601 aacgtcgtct tgccggcgcc gttcgggccg ataatggcgc ggagctcgcc gcgcgggacg
     9661 acgagcgaca cgtcgcgcac cgcgatcaga tcgccgaagc gcttgctcac ggattcgact
     9721 ttgagaatgg cgccggacgg ctcggcttcc tctgtggcgc tcgcccctcc ctcggcgctc
     9781 atgccgcggc ccgcttgccg acaaagccga gcaggccgcg cggaaagaac aaaacgacaa
     9841 ggacgaacaa taggccgaca aaggacatcc aattgacggt cacgctggaa aggtagtcct
     9901 gcacgacgac gaaaacgacg gcgccgagga gcgggcccca gaaattgcgc atgccgccca
     9961 tcaccgccat catcacgaag tcgccggatt ggctgtaatg caggccgcgc ggatcggcga
    10021 aattgttgac gagcgcatag agcgcgccgg cgaagcccat gaacaagcaa gaaagggtga
    10081 aagcgatcca gatatggcgc tcgaccgcga ggccgaggaa tcgcgcgcgg cgctcgttct
    10141 cgcgaatggc gatcatcgcg cgcccaaatg gcgagcgcaa aataaagccc atcaccgcga
    10201 cgagcagggc gaagcaaagc agcacgaaat agtagaaagc cgtctcgttg gagagaatat
    10261 cgatcctcgc gaatccgaga tcgagcggct ggcgcgcgaa tccgcgcaaa ccgtcgtcgc
    10321 cgccggtcag cgaactccat tgaaaggcga tatagtacag gacctggccg aagg
//
LOCUS       DATGJW010000162         2167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_29901, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000162 DATGJW010000000
VERSION     DATGJW010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2167
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29901"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..939
                     /gene="paaG"
                     /locus_tag="VKU03_04950"
     CDS             133..939
                     /gene="paaG"
                     /locus_tag="VKU03_04950"
                     /EC_number="5.3.3.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004300 - enoyl-CoA hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0010124 - phenylacetate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA
                     isomerase PaaG"
                     /protein_id="HLJ70642.1"
                     /translation="MAESTILVEDRAAGSARYRVITLNRPERLNAFIAPMHQALRRAL
                     IEAERDASCRAVLLTGAGRGFCAGQDLSERAVNDGGPPPDLGATLDALYNPLIRRLRA
                     MPLPVVCAVNGPAAGAGANIALAGDIVLAARSATFLQAFARIGLSPDAGGTFFLTRTV
                     GPARARALAMLAEPLGAEQAADWGLIWKAVDDAMLMDEAHAICAKLASGSAAALAAIK
                     RGIETAATNSLTDQLDLERDAQRELGRSGDYREGVRAFLEKRPAVFGPRR"
     gene            1095..1718
                     /locus_tag="VKU03_04955"
     CDS             1095..1718
                     /locus_tag="VKU03_04955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ70643.1"
                     /translation="MTARAAKMEATRRRILDCAADLYMRRSIDDFTLEEVAHCAGATV
                     QTILRIHGSRDRLLLAALDKLVQIGVPLKPTPPGDIDAAVGAIFDLYEATGEVILKEL
                     ADEHRRPELKKMLDEGRAHHRDWVQLAFAPQLAACPSAAARMLLLNALIVATDITVWA
                     KLRKDNALARPQAEAVVRRIIESVVEREDRHGEASVAELVGRRKSSA"
     gene            1666..>2167
                     /locus_tag="VKU03_04960"
     CDS             1666..>2167
                     /locus_tag="VKU03_04960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70644.1"
                     /translation="MAKLLWLNWSGGGNLPPSLGVARVLRERGHDVAFAGRPEMVPRV
                     RTAGFRAIEITQAYAQAEGYPSGSHMTRMACYLTSPAVEAQIQSIVAAEQPDVALIDG
                     MVVAALLHAHEFGAPSAVFVHTFVFRQLDMWRRIFRTFSDMRQKAGFSALPALDELWR
                     LRERLIS"
BASE COUNT          350 a          712 c          712 g          393 t
ORIGIN      
        1 tagagattcg cggggggcgc gtaccttccg atttctgcgc ttcccctcat ccggcgcttc
       61 gcgccacctt cgccccgcct tgcgaggaga aggggatgga atcgatggta cgatcaagct
      121 gagggaaggg aaatggctga aagtacaatc cttgtcgaag atcgcgccgc cggctcggcg
      181 cggtatcgcg tcattacgtt gaatcggccc gagcggctca acgcgttcat cgcgccgatg
      241 catcaggcgc tgcggcgggc cttaatcgag gcggagcgcg acgcttcctg ccgcgccgtc
      301 cttttgaccg gcgccggccg cggcttctgc gctgggcaag atctttccga gcgcgcggtc
      361 aatgacggcg ggccgccgcc cgatctcggc gcgacgctcg atgcgctcta caatcctctg
      421 atccgccgcc tccgcgccat gccgctgccg gtcgtctgcg cggtgaacgg gccggccgcc
      481 ggcgccggcg ccaacatcgc gctcgccggc gacatcgtcc tcgccgcgcg ctcggcgacg
      541 tttctccagg ccttcgcgcg gatcggcctt tcgcccgacg ccggcggaac gttcttcctg
      601 acgcgaacgg tcgggccggc gcgggcgcgc gctcttgcta tgctcgccga gccgctcggc
      661 gccgaacagg cggcggattg gggcttgatc tggaaagcgg tggacgacgc aatgctgatg
      721 gatgaagcgc acgcgatctg cgcgaagctt gcatcgggct cagccgccgc gttggccgcg
      781 atcaagcgcg gcatcgagac ggcggcgacg aacagcctga ccgatcagct cgatctcgag
      841 cgtgacgcgc aacgcgaact cggccggtcc ggcgattacc gcgaaggcgt gcgcgccttt
      901 ctcgaaaagc ggccggctgt tttcggcccg cgccggtagg acaatccgaa cgctgcgctc
      961 gccgtcggcg gggagacccc tccgcgcgca aatccgcccc gcgcgcaggc ggcaaaatcg
     1021 tgttaattcg attgacatta aattattttc gatttagtgt tattaacatg aaatcgaaga
     1081 aacgccctta tgcgatgacc gcgcgggctg cgaaaatgga ggcgacgcgc cggcgcattc
     1141 tcgattgcgc cgccgatctc tacatgcggc gaagcatcga cgatttcacg ctcgaagagg
     1201 tcgcgcattg cgccggcgcg accgtgcaga cgattttgcg cattcatggc agccgcgacc
     1261 gactcctcct cgccgcgctc gataagctcg tccagatcgg cgtgccgctg aagccgacgc
     1321 cgccgggcga cattgacgcg gcggtcggcg cgattttcga tctctacgag gcgaccggcg
     1381 aggtcattct caaggagctg gcggacgagc atcgccgtcc cgaactcaag aagatgctgg
     1441 acgaaggccg cgcccaccat cgcgattggg tccagctcgc cttcgcgccg cagctcgcag
     1501 cttgcccgag cgcggcggcg cgcatgctgc tgctgaacgc tctgatcgtc gctacggaca
     1561 ttactgtctg ggccaagctg cgcaaggaca acgctctggc gcggccgcag gccgaagccg
     1621 tggttcggcg catcatcgaa tcggtcgtgg agagggagga caggcatggc gaagcttctg
     1681 tggctgaatt ggtcgggcgg cggaaatctt ccgcctagcc tcggcgttgc gcgggtcttg
     1741 cgcgagcgcg ggcatgacgt ggcgttcgcc gggcgtccgg aaatggttcc gcgcgtcagg
     1801 accgcgggct tccgcgccat tgaaatcacc caagcctatg cgcaggccga aggctatccg
     1861 tcgggcagcc acatgactcg gatggcctgc tatctcacct cgccggccgt cgaagcccag
     1921 atccaatcga tcgtcgccgc cgagcagccg gatgtcgcgc ttatcgacgg catggtcgtt
     1981 gcggcgctgt tgcatgctca cgaattcggc gcgccgagcg ccgttttcgt ccatactttc
     2041 gttttccgcc agctcgatat gtggcgaagg attttccgaa ccttcagcga catgcgccag
     2101 aaggcgggct tctcggcttt gcccgcgctc gacgagctct ggcgtctgcg cgagcgcctg
     2161 atttcga
//
LOCUS       DATGJW010000163         4395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_134492, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000163 DATGJW010000000
VERSION     DATGJW010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4395
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134492"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..955)
                     /locus_tag="VKU03_04965"
     CDS             complement(5..955)
                     /locus_tag="VKU03_04965"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70645.1"
                     /translation="MPFLLRRYGTLIGFLVLIVFFWAELPDTFLTARNLLNITQQMSM
                     LAVVSATMTVVMAMGDFDLSVGSIASLAGVVAAMIFVAGGSPFLAVPAALAAGLAAGA
                     VNGFLVAYVGILPFIATLGALTVYSGLAFRLSDGKTIFGSVIPASFGGFARGGPDVGG
                     FAIPNLTLVAALALVLVWLFLEQTITGRRLYAIGGNTEAARLAGVRIKPLKVLAFALS
                     GFGAAAAGLMIASRVASANPTQGAGLMLDAIAAVFLGMTMSEEGEPRVLYTLLGVMML
                     GVLGNGLTQMNVDSYVRDMITGLIIVAAVAAGSLGRRREG"
     gene            complement(959..2461)
                     /locus_tag="VKU03_04970"
     CDS             complement(959..2461)
                     /locus_tag="VKU03_04970"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ70646.1"
                     /translation="MSLLSIGHLRKAYGGAPALKDASLELFAGETLALMGENGAGKST
                     LIKILAGAVQPDGGDIRIEGAAASIRSPEEAHRRGLRFIHQELNVVAGLSVAENIFLG
                     RVYPRRLGFVDWGALNARASAALATLGVDHLSPKTIVAKLPVGDRMLVRIAAAFLEDD
                     SARARIFVMDEPTAALTKEESERLFKIIGALRARGCGILFVSHRLDEVLAIADRISVL
                     RDGETCATLNAKDATKARLIELMTGRKDVTGDRGPPAPRGAPIVLDVDGLSGDGLTGI
                     SFKLHKGEILGFTGLAGAGPERLIRALVASPGAGTVTLDGEPAGLRGPADAWARGIAL
                     TPRERRAEGLLLKHDIASNVSLPHLSRLSRMRWFLDRRKERARAADLGRRVRLKASGP
                     RQKVRTLSGGNQQKVLLARAVARAPRVLLLDEPTRGVDVGAKFDIYAFLREIAEGGAS
                     VVVVSSDHEELLRLCSRVAVLREGRIAATVPAEGLTPERLLALCYGDPKD"
     gene            complement(2500..3420)
                     /locus_tag="VKU03_04975"
     CDS             complement(2500..3420)
                     /locus_tag="VKU03_04975"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ70647.1"
                     /translation="MNRIRLVALAAAFICAATSALAGAKITIVTPYLAQPGTQYYVEA
                     FQEVAKQNGWTVNVVDTKGDIAAVVSRIEDAANDKVDAIVLNANPAQVTAGLETAKAA
                     GVPVFGMDAGSSPLLVANVTSNGYEMAATTASYVVNRIGGKGNVVMFVYDAFPPVQVR
                     GVIADAIFKNTPDIKVIARVTPDVQDGGIADSRAKMEAILAAHPMKGSIAAVWAAWDQ
                     PALGALQAIEAAGRTDEGIVIVGMDANPQARAAIAKDGNFEASMAQDFKGIGRTVADL
                     VKRHLAGEKIKSSVIYVPTTLITAENVKQQ"
     gene            3548..4273
                     /locus_tag="VKU03_04980"
     CDS             3548..4273
                     /locus_tag="VKU03_04980"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF012511.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HLJ70648.1"
                     /translation="MRRRLVVYFPLADRAVPDELIDLYAGAGVDIVEFGWPARNPYLD
                     GPDVWASMARALSGAPAAALKAACDRLARKSESPKTLVMCYAESDHPALVEPGVFDRA
                     DGVLAVAPPNDARRAAIEREARKSGAAIAVFVPMAADPGDEAAARHADFYVMLQAAPG
                     LTGPRASLDPANAGRIASLRASGVTAPILLGFGVSNGEQARAAVGFGADGVVVGSAAL
                     RAALAGRAELKALLKDLRMGLDG"
BASE COUNT          706 a         1489 c         1498 g          702 t
ORIGIN      
        1 atgcctagcc ctcgcgcctg cgtccaaggc tgcccgcggc gacggcggcg acgatgatca
       61 agcccgtgat catgtcgcga acgtagctgt ccacgttcat ttgcgtgagc ccgttgccga
      121 ggacgcccag catcatgacg ccgagcaagg tgtagagaac ccgcggctcg ccctcctcgc
      181 tcatggtcat gccgaggaac accgccgcga tcgcgtcgag catcaggcca gcgccctgcg
      241 tcgggttggc cgaggcgacg cggcttgcga tcatcagccc cgcggccgcc gcgccgaagc
      301 cggaaagcgc gaaggcgagg accttgagcg gcttgatccg gacgccggcg aggcgcgcgg
      361 cctcggtatt gccgccgatc gcgtagagcc gccggccggt gatggtctgc tcgaggaaga
      421 gccagacgag gacgagcgcc aaggcggcga cgagggtgag gttgggaatg gcgaacccgc
      481 caacgtcggg cccgccgcgg gcgaagccgc cgaaggaggc cggaatgacc gagccgaaga
      541 tcgtcttgcc gtcggagagg cgaaaggcga ggccgctata gacggtcagc gccccgagcg
      601 tcgcgatgaa agggagaatt ccgacatagg cgacgaggaa gccattgacg gcgccggccg
      661 cgagccctgc cgcgagcgcc gccggcacgg cgaggaaggg cgatccgccc gcgacgaaaa
      721 tcatggcggc gacaacgccg gcgaggcttg cgatcgaacc cacgctcaaa tcgaagtctc
      781 ccatcgccat gaccacggtc atcgtcgccg agacgacggc gagcatgctc atctgctggg
      841 tgatgttgag gagattgcgc gcggtgagaa aggtgtccgg cagctcggcc caaaagaaga
      901 cgatgaggac gaggaagccg atcagcgttc cataacggcg caggaggaac ggcatcggtc
      961 agtccttggg atcgccgtag caaagcgcga gcaggcgctc tggggtcaat ccctcggccg
     1021 ggaccgtcgc cgcgatccgc ccctcgcgca ggaccgcgac gcgcgagcac agacgcaaga
     1081 gctcctcgtg gtcggatgac acgacgacca cgcttgcgcc gccttcggcg atttctcgca
     1141 ggaaggcgta gatgtcgaac ttcgccccga cgtcgacgcc gcgcgtcggt tcgtcgagga
     1201 gcagcacgcg gggcgcgcgg gcaaccgcgc gcgcgagcag gactttctgc tggttgccgc
     1261 ccgagagcgt cctcaccttc tggcgaggcc cgctggcctt gagacgcacg cggcggccca
     1321 ggtccgcggc ccgagcgcgc tccttgcggc gatcgagaaa ccagcgcatg cggctcagcc
     1381 gtgaaaggtg cggcagcgac acgttgctgg caatgtcgtg cttgagcaga agaccctcgg
     1441 cgcggcgttc gcggggggtg agcgcgattc cgcgcgccca cgcgtccgcc ggccccctta
     1501 agccggcagg ctcgccatcg agggtcacgg tccccgcgcc ggggcttgcg acaagcgcgc
     1561 ggatcaggcg ctcgggaccg gcgccggcca gcccggtgaa accgaggatc tcgcccttgt
     1621 gcagcttgaa tgagatgcca gtcaggccgt cgccggagag accgtcgaca tcaaggacaa
     1681 tgggcgcgcc gcgcggcgcc ggcgggccgc ggtcgccggt gacgtccttg cggccggtca
     1741 tcagctcgat gagcctcgcc ttggtcgcgt cttttgcgtt cagggtcgcg caggtctccc
     1801 cgtcccgcaa aaccgagatc cggtcggcga tcgccagaac ctcgtcgagc cgatgcgaga
     1861 cgaacaggat tccgcagccg cgcgcgcgaa gcgcgccgat gatcttaaac agccgctccg
     1921 attcctcttt ggtcagcgcg gcggtcggct cgtccatgac aaagatgcgc gcccgcgcgg
     1981 aatcatcctc gaggaaggcg gccgcgatcc tgaccagcat ccggtcgccg accggcagtt
     2041 tcgcgacgat cgtcttcggg gagaggtggt caactccgag cgtcgccagc gcggcgcttg
     2101 cgcgcgcgtt gagcgctccc caatcgacga agccgagccg gcgcggatag acgcggccga
     2161 gaaaaatgtt ctcggcgacg ctgaggccgg cgacgacgtt cagctcctgg tggatgaatc
     2221 tgaggccgcg ccgatgggcc tcctccgggc tgcggatcga agccgccgcg ccctcgatcc
     2281 gaatgtcgcc gccgtccggc tgaaccgcgc cggccaggat cttgatcagc gtgctcttgc
     2341 ccgccccgtt ctcgcccatg agggcgagcg tctctccggc gaaaagttcg agggaagcat
     2401 ccttgagcgc cggcgcgccg ccgtaggcct tgcgcaaatg accgatcgaa agcagactca
     2461 ttgcatgtcg cggcgccgcg gtccgcctgc gcgcggcgtc tattgctgct tcacgttctc
     2521 cgcggtgatg agtgtcgtcg gcacatagat cacgctactc ttgatctttt cgccggccag
     2581 atggcgcttg accagatcgg cgacggtgcg gccgatgccc ttgaagtcct gcgccatcga
     2641 ggcctcgaaa ttgccgtcct tggcgatcgc cgcgcgcgcc tgcgggttgg cgtccatgcc
     2701 gacgatgacg atgccttcgt cggtccggcc ggccgcctcg atcgcctgca gcgcgccaag
     2761 cgccggctga tcccaggccg cccataccgc cgcgatcgag cccttcatgg gatgggcggc
     2821 gagaatcgcc tccatcttgg cccgcgagtc ggcgatgccg ccgtcctgaa catcgggggt
     2881 cacgcgggcg atgaccttga tgtcgggcgt gttcttgaag atcgcgtcgg cgatcacgcc
     2941 gcgcacctgc accggcggga acgcgtcgta gacgaacatc accacgtttc ccttgccgcc
     3001 gatccggttg accacgtagg aagcggtggt cgccgccatt tcgtagccgt tgctggtcac
     3061 gttggcgaca agaagcgggc tcgagccggc gtccatgccg aacaccggca cgcccgccgc
     3121 cttcgcggtc tccagaccgg cggtgacctg cgcgggattg gcgttgagga caatcgcatc
     3181 caccttgtcg ttggcagcgt cctcgatgcg gctcacgacc gccgctatgt cgcctttcgt
     3241 gtcgacgaca ttaacggtcc agccgttctg cttggcgact tcctggaagg cctcgacata
     3301 gtactgggtg cccggctggg cgagataggg cgtcacgatc gtgatcttcg cgcccgcgag
     3361 cgcggatgtc gccgcacaga tgaacgccgc cgcaagcgcg accagtctta tccgattcat
     3421 tgtcttgccc tcgtccaacg gcgtcctccc gccggagacg acacgtagca cccggctttc
     3481 cttcacttca actggaacct tcgatcgcgc tccttgcgct tcggaaaggc gatgccatag
     3541 aagcgcgatg cgccgccgtc tggtcgtcta tttcccgctc gcggaccgtg ccgttccaga
     3601 tgaattgatc gatctttacg ccggcgccgg ggtcgatatc gtcgagttcg gctggccggc
     3661 gcggaacccc tatctcgacg ggcccgatgt ctgggcgtcg atggcgcggg cgctttccgg
     3721 cgctccggcg gcggcgctca aagcggcgtg cgaccggctc gcacgcaagt ccgaaagccc
     3781 gaagacgctg gtcatgtgct acgccgagag cgatcatccg gcgctggtcg aacccggcgt
     3841 tttcgatcgc gccgacggag tcctcgcggt cgctccccca aatgacgcgc gccgcgcggc
     3901 gattgaacgc gaggcgcgga aatcgggcgc cgcgattgcg gtgttcgtgc ccatggccgc
     3961 cgatcccggc gacgaggccg ccgcgcgcca cgcggatttc tatgtcatgc tgcaagccgc
     4021 gcctgggctc actggaccgc gcgcatcgct cgatcccgca aacgccggac gcatcgcgag
     4081 cctgcgcgcg tccggcgtga ccgccccgat ccttctcggc tttggcgtct cgaacggcga
     4141 gcaggcgcgc gccgcggtgg gttttggcgc cgacggggtc gtcgtcggct cggcggcatt
     4201 gcgcgccgcg ctcgccggac gggccgaact caaggcgctc ctgaaagact tgcggatggg
     4261 gctcgatggc tgaacgcggc gatctttttc tcggcttcga cgtcggcacc acgaccgtca
     4321 agggcgcgct gttcgacatc gagggccggc tggtcgccca tgcggggcgc ccatatccga
     4381 cctctcgccc agcgg
//
LOCUS       DATGJW010000164         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1067969, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000164 DATGJW010000000
VERSION     DATGJW010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1067969"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2382)
                     /gene="ppdK"
                     /locus_tag="VKU03_04985"
     CDS             complement(<1..2382)
                     /gene="ppdK"
                     /locus_tag="VKU03_04985"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HLJ70649.1"
                     /translation="MTKWVYSFGGGAAEGEGAMRELLGGKGANLAEMANLGLPVPPGF
                     TITTEVCAFFYANGKTYPPDLKEAVEAALTRVGELTGRAFGDASNPLLVSVRSGARAS
                     MPGMMDTVLNLGLNDSSVKTLAATSGDERFAYDSYRRFIQMYSNVVLEIEHHNFEEIL
                     QERKDRRDYLLDTELSAEDWREIIAQYKAVVEAETGKPFPQDPRDQLWGAIGAVFSSW
                     MNQRAITYRRLHSIPESWGTAVNVQAMVFGNMGETSATGVAFTRNPSTGAKELYGEFL
                     VNAQGEDVVAGIRTPQNITEAARMAAGSDSPSLETLMPDVFRQFRETTERLEKHYRDM
                     QDLEFTVERGKLWMLQTRNGKRTARAALRVAVDMAEEGLIGKSEAVLRVEPASLDQLL
                     HPTLDPKAPRKPLASGLPASPGAASGEIVFSADEAEDLKNRGRKVILVRVETSPEDIH
                     GMHAAEGILTTRGGMTSHAAVVARGMGKPCVSGAGSVRVDYAAQTLTAAGVTLKKGDV
                     ITIDGGDGQVFQGAVAMLKPELSGDFLTLMSWADAHRRMKVRANAETPNDARVARGFG
                     AEGIGLCRTEHMFFEGERIVAVREMILSTDEAGRRAALAKLLPIQRKDFAELFEIMTG
                     LPVTIRLLDPPLHEFLPHAEEEVAEVAKAMGVSREKLRARAAELHEFNPMLGFRGVRL
                     MVRYPEIAEMQARAIFEGAVEAGRKTGSAVAAEIMVPLVMTRGELDLIKARIVAMAEA
                     VAKETGVAVPYSIGTMIELPRAALRAGDIAESAEFFSFGTNDLTQTTLGVSRDD"
     gene            complement(2517..3092)
                     /locus_tag="VKU03_04990"
     CDS             complement(2517..3092)
                     /locus_tag="VKU03_04990"
                     /inference="COORDINATES: protein motif:HMM:NF015674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF308 domain-containing protein"
                     /protein_id="HLJ70650.1"
                     /translation="MSDPTRAVEIHSLGGALHRLHEKWGAIVAFGVLLIALGLAAIVF
                     ALAATVATVVVNGVVFLIAGVAEIGVGMHSQAWGRFFFWVIGGLLYLIAGLTCIFNPI
                     LASVILTLILGAGLIAAGIVRIVFGFQLPGDHPRSLVFLAGAVTTILGLIIVGHWPAD
                     SVIVLGTLLGVDLLFHGAGWVSFGLGLRARR"
     gene            complement(3146..>3503)
                     /gene="hrcA"
                     /locus_tag="VKU03_04995"
     CDS             complement(3146..>3503)
                     /gene="hrcA"
                     /locus_tag="VKU03_04995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017482677.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HLJ70651.1"
                     /translation="EQTQQLIVRGQANLLEDPNARDDLERIRRLFADLETKTDVIDLL
                     HRAESGDGVRIFIGSENKLFSLSGSSMIAAPLRNGERRIAGVLGVIGPTRLNYARIVP
                     VVEYTARVLGGLLAAR"
BASE COUNT          627 a         1170 c         1125 g          581 t
ORIGIN      
        1 gtcatcgcgc gacacgccaa gcgtcgtctg ggtcagatcg ttggtgccga aggaaaagaa
       61 ttcggcgctt tccgcgatgt cgccggcgcg cagggcggcg cggggaagct cgatcatcgt
      121 gccgatcgaa taaggcacgg cgacgcccgt ctccttcgcc accgcctccg ccatggcgac
      181 gatgcgcgct ttgatcaaat cgagttcgcc gcgcgtcatc accagcggaa ccatgatctc
      241 agcggcgacc gcggagccgg tcttgcggcc ggcttcgacc gcgccttcga aaatcgcccg
      301 cgcttgcatc tcggcgattt ccggataacg aaccatgagc ctgacgccgc gaaagccgag
      361 catcggattg aactcgtgca attcggcggc gcgggcgcgc agcttctcgc ggctgacgcc
      421 catcgccttg gccacttcgg cgacctcctc ctcggcatgc ggcaggaatt cgtgcaaggg
      481 cggatcgagg aggcggatcg tcaccggcaa gccggtcatg atttcgaaga gctcggcgaa
      541 gtccttgcgc tggatcggca gcaatttggc gagcgccgcg cgccggccgg cttcgtcggt
      601 cgagaggatc atctcgcgca cggcgacgat gcgctcgccc tcgaagaaca tatgctcggt
      661 gcggcaaagg ccgatcccct cggccccgaa gccgcgcgcg acgcgcgcgt cgttcggcgt
      721 ctcggcattg gccctgacct tcatccgccg atgcgcgtcg gcccagctca tcagggtgag
      781 aaagtcgccg ctcagttccg gcttcagcat cgcgaccgcg ccttgaaaga cctggccgtc
      841 gccgccgtcg atcgtgatca catcgccttt cttgagcgtg acgccggccg cagtcaaagt
      901 ctgcgccgcg tagtcgacgc gcaccgagcc ggcgccggag acgcagggct tgcccatgcc
      961 gcgcgccacg accgcagcat gcgaggtcat gccgccgcgc gtcgtcaaaa tgccctcggc
     1021 ggcgtgcatg ccgtgaatgt cttccggcga ggtctcgacc cgcaccagga tcactttgcg
     1081 gccgcgattc ttcaaatcct cggcctcgtc ggcggaaaag acgatctcgc cgctcgctgc
     1141 gcccggggaa gccggaagtc ccgaggcgag cggcttgcgc ggcgccttcg gatcgagggt
     1201 cggatgaagc aattggtcga gcgaagccgg ctcgacgcga agcaccgcct cgctcttgcc
     1261 gatcaggccc tcttccgcca tgtcgacggc gacgcgcaaa gcggcgcgcg ccgtgcgctt
     1321 gccgttgcgg gtctgcagca tccacagctt gccgcgctcg acggtgaatt cgaggtcctg
     1381 catgtcgcgg taatgctttt cgagcctctc cgtcgtctcg cggaattgcc ggaacacgtc
     1441 cggcatcagc gtttcgaggg acggactgtc ggatcccgcc gccatgcgcg ccgcctcggt
     1501 gatgttctgc ggcgtgcgga tgccggcgac cacgtcctcg ccctgcgcgt tgacgaggaa
     1561 ttcgccgtaa agctccttcg ccccggtcga cggattgcgg gtgaaggcga cgccggtcgc
     1621 cgacgtctcg cccatattgc cgaagaccat cgcctggaca ttgaccgccg tgccccagct
     1681 ttcggggatc gaatgcaggc ggcgataggt gatggcgcgc tggttcatcc aggacgagaa
     1741 gaccgcgccg atcgcgcccc acaactggtc gcgcggatcc tgcggaaacg gcttgccggt
     1801 ttcggcttcg accaccgcct tgtactgggc gatgatctcg cgccaatcct cggcggagag
     1861 ctcggtgtcg agaagataat cgcggcggtc tttgcgctcc tgcagaatct cttcgaaatt
     1921 gtgatgctcg atctccagca ccacgttcga atacatctgg atgaagcggc ggtagctgtc
     1981 ataggcgaaa cgctcgtcgc cggacgtcgc ggcaagcgtt ttgacggagg aatcattcag
     2041 gccgagattg agcaccgtgt ccatcatgcc gggcattgac gctctcgcgc cggagcggac
     2101 cgagacgagc agcggattgc ttgcgtcgcc gaaggcgcgg ccggtcaatt cgccgacgcg
     2161 cgtgagcgcc gcctcgaccg cctctttcaa gtccggcgga taggttttcc cattggcgta
     2221 aaagaacgcg caaacctcgg tcgtgatggt gaagccgggc ggcacgggca ggccgagatt
     2281 cgccatttcg gcgagattgg cccccttgcc gccgaggagc tcgcgcatcg cgccctcgcc
     2341 ttcggccgcg ccgccgccga aggaatagac ccatttcgtc atgccatacc ccttagagag
     2401 cgccgttcgc cgagccttga acggcgcgga gagccgcttc gttccggaaa cagaggctta
     2461 ggcggctcgc aaccggcggc tcgctcattc catcgttctt gtatcagcgc ggcccgtcaa
     2521 cgccgcgcgc gcaggccgag cccgaagctc acccagcccg cgccatggaa taaaagatcg
     2581 acgccgagca gcgtgccgag cacgatgacg ctgtcggccg gccaatgccc gacaatgatc
     2641 aggcccaaaa ttgtggtcac cgcgccggcg agaaacacca aagagcgggg atgatcgccc
     2701 gggagctgaa agccgaacac gatgcgtacg attcccgcgg cgatcaggcc ggcgccgaga
     2761 atcaaggtca agatcaccga ggcgaggatc ggattgaaaa tgcaggtcag accggcgatc
     2821 agatagagca agccgccgat cacccagaaa aagaagcggc cccaggcttg cgaatgcatg
     2881 ccgacgccga tctcggcgac gccggcgatg aggaacacca cgccgttaac gacaacggtc
     2941 gcaaccgtcg ccgccaaggc gaagacgatc gccgcgagac cgagcgcgat cagcaagacg
     3001 ccgaacgcga cgatcgcgcc ccatttctcg tgcagccggt gcagcgcgcc gccgagactg
     3061 tgaatctcga ccgcgcgggt tggatcgctc atgaaagcct cccgtctcca ctctaaccga
     3121 tttagccatg cccgccaccc gcgcttcagc gcgcggcgag caagcctccc agcacgcgcg
     3181 cggtatattc gaccaccggc acgatgcgcg catagttcaa ccgggtcggc ccgatgacgc
     3241 cgagcacgcc ggcgatccgg cgctcgccgt tgcgcagcgg cgccgcgatc atcgacgagc
     3301 cggacagcga gaacagcttg ttctcggagc cgatgaagat gcggacgccg tcgccgcttt
     3361 cggcccggtg cagcaggtcg atcacgtcgg ttttggtctc gagatcggcg aaaagccggc
     3421 ggatgcgctc gagatcgtcg cgcgcgttcg gatcctcgag caggttggct tggccgcgca
     3481 cgatcaattg ctgggtctgc tcc
//
LOCUS       DATGJW010000165        10135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_756578, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000165 DATGJW010000000
VERSION     DATGJW010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10135
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756578"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(227..931)
                     /locus_tag="VKU03_05000"
     CDS             complement(227..931)
                     /locus_tag="VKU03_05000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018063044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HLJ70652.1"
                     /translation="MSAQSAPRAAPVDTLSPDAQRVADAFAASIAARRAFDEPYRHYL
                     IENVFPADILDALADLPFAAPRLDGVSGKRELHNDQRSYFDAAAMARFPAMRAVAEAL
                     QSRRVVRLIHDAFGAPIDDTFLRLEYAQDVDGFWLEPHTDLGVKKFTCLIYLSQGPGH
                     DELGTDIYRSKERHYGRSPFKRGGAMIFVPADNTWHGFEKRPIQGLRRSVILNYVTHE
                     WRAREQLSFPQEPVRV"
     gene            complement(1255..1497)
                     /locus_tag="VKU03_05005"
     CDS             complement(1255..1497)
                     /locus_tag="VKU03_05005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2312 domain-containing protein"
                     /protein_id="HLJ70653.1"
                     /translation="MKSDVVNAGHLRSFIERVEKLEEEKKALSDDIKDVYAEAKGNGF
                     DVKILRKIVSMRRQDRDKRREEQEILDLYLAALGMV"
     gene            complement(1583..2446)
                     /locus_tag="VKU03_05010"
     CDS             complement(1583..2446)
                     /locus_tag="VKU03_05010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HLJ70654.1"
                     /translation="MDLSGAAGTASAGGAFQPVEFIEGALDAGVIILCDHASNALPPA
                     YGDLGLPPSAFARHIAYDIGAAWLTRRLARLLNAPAVLSTFSRLLIDPNRGADDPTLV
                     MQISDGAIVPGNARVGAEEIALRRDLYWRPYRQACVDLVEQMSASGRGPAVLSVHSFT
                     PAWRGRPRPWKVGVLWDMDPRLPEPLLQALAREPDLQPADEAVGDNEPYDGALAGDTI
                     DAVATARGLANALIEIRQDLIATEPDAEAWAARLARLIAPILALPNIHERIDFGSRAA
                     GKARRRKEQRG"
     gene            2562..3443
                     /locus_tag="VKU03_05015"
     CDS             2562..3443
                     /locus_tag="VKU03_05015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ70655.1"
                     /translation="MNSAITDAQPAKSYGAPFLALVAGALAMGASPIFVRLADVGPFT
                     SAFWRVALALPILYAWMRVAERDGGRSRSFSRAIVLSGLAFAGDLFFWHLSIVSTTVA
                     NATFFATTAPIWVVIFGWLLFRTRVSAGVLAGLALCLAGGGALLAQSLNLHAGRAFGD
                     LCGLTTGVFFGLYFLAVQAARRESSAARVTFEATLITAALLLIVALVLERRFWPHSLP
                     GLAALLAMAWISHAGGQGLLAAALGRLPAAFSSLVIFLEAIAAACFAWAALNEPVSAV
                     QAAGGLAILLGIYVARP"
     gene            3507..3956
                     /locus_tag="VKU03_05020"
     CDS             3507..3956
                     /locus_tag="VKU03_05020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HLJ70656.1"
                     /translation="MTEQTGESREKARKSIGLLACSIAMLFALAPARADFRMCNNTSN
                     RVSVALAYTDGQNWTSEGWWNLKPSDCDILLRGTLAAEFYYVYAMDERGGEWKGKAFM
                     CTSDREFKIDGRQDCYVRGYDRTGFFEVDTGKDARDWTVQLTDPTSP"
     gene            4026..5459
                     /gene="pyk"
                     /locus_tag="VKU03_05025"
     CDS             4026..5459
                     /gene="pyk"
                     /locus_tag="VKU03_05025"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610869.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HLJ70657.1"
                     /translation="MRRRRRVKIIATLGPASLDRATVASLFEAGADVFRINMSHATHD
                     GMRARVAMIRSLEKEYGRPIGILIDLQGPKLRVGEFETGAVLLRKGEAFTLDSDPTPG
                     DQTRVCLPHPEILDSLAPGHRLLIDDGKVLLKVTSVAPGKAATIVEVAGRVSNKKGVS
                     LPDTTIPTSAMTEKDREDLDAALQEDIDWIALSFVQRPEDVAELRMIVGGRAMVMAKI
                     EKPQAIARLDEIMEVSDALMVARGDLGVEMPLEQVPGLQKRITRTARRLGKPVVVATQ
                     MLESMISSPVPTRAEVSDVATAVFEGADAVMLSAESASGQYPREAVATMSRIAEQIES
                     EEIYPAVITAQRASPERTGADAIAVATRDVAETLDLKLICAWTASGSTGLRIARERPR
                     SSIVALTPNISAARRLALVWGVHAIVTEDARDERDMAERACRLAATEGFAEVGERIII
                     VAGVPFGTPGATNNMRIAYVEAHDAAE"
     gene            complement(5763..6470)
                     /locus_tag="VKU03_05030"
     CDS             complement(5763..6470)
                     /locus_tag="VKU03_05030"
                     /inference="COORDINATES: protein motif:HMM:NF024820.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ70658.1"
                     /translation="MIRLGSIVAVALLAAALGASAAAPEEGRPALRAPGLSPVPLPPR
                     AHGVSPGSENAAPRSSKPEDSTQEKPPAPRTRAELLNDLYARLAASKDADETAGLIGA
                     IDRLELESGSDAGDLLMARAVAAMAAKNYDVALALLDKLVDLHPEWAEAWNKRATVRF
                     LVDDDRGSMADIAHVLKLEPRHIGALAGMGVILEREGFRDDALRAYQRALQIAPQLKS
                     LKDSVERLTKAVQGEDL"
     gene            complement(6575..6700)
                     /gene="ykgO"
                     /locus_tag="VKU03_05035"
     CDS             complement(6575..6700)
                     /gene="ykgO"
                     /locus_tag="VKU03_05035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012177066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type B 50S ribosomal protein L36"
                     /protein_id="HLJ70659.1"
                     /translation="MKVRNSLKSLRKRHRDNQLVRRKGRVYIINKTQKRFKARQG"
     gene            complement(6827..7279)
                     /locus_tag="VKU03_05040"
     CDS             complement(6827..7279)
                     /locus_tag="VKU03_05040"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927677.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLJ70660.1"
                     /translation="MPDAKAPRPSPLGRAAFARFVPIATRWSDNDVYGHVNNVAYYSF
                     FDTAVNALLVEAGLLDPAESPIVGLVVDSNCAFFSSLAFPDPIEVGVAVEEIGRSSVR
                     YLLAVFKAGAEKAAAQGRYTHVYVERASRKPTPIPDGHRRLMERLRIR"
     gene            complement(7283..7870)
                     /locus_tag="VKU03_05045"
     CDS             complement(7283..7870)
                     /locus_tag="VKU03_05045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin transporter BioY"
                     /protein_id="HLJ70661.1"
                     /translation="MNDNRRPTLAAFLWPERAASRAGVAALAVIGTLALTLSAKVQVP
                     FYPVPMTLQTLVVLILGAAFGARLAGATVALYLAEGVIGLPVFAGASAGPTYMAGPTG
                     GYLAGFLAAAVAIGYLAERGWDRSGPRLLAAMALGHALIFAFGFLWLAGLIGAEKAWA
                     AGVAPFFAATLVKTLLAWALVGAFWSAALGLRSRA"
     gene            8062..8976
                     /locus_tag="VKU03_05050"
     CDS             8062..8976
                     /locus_tag="VKU03_05050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007568850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLJ70662.1"
                     /translation="MSGDDDLIFDAEDAEAGVVKRVSPLVRRLIAGNPSPFTFTGTCS
                     YIVGQGSVAIVDPGPLDEAHIAALLAAMRGESVRHILVTHTHRDHSPAAARLKAETGA
                     AIVGARPFAPKRGAGEGKGLDAAHDLAYAPDHALGDGERVAGEGYTLEAVATPGHAAN
                     HLCFALIEENSLFSGDHVMAWSTTVVAPPDGSMSDYMASLEKLRARGETLFWPGHGGP
                     VRNPQRYLRGLAHHRRQREAAILARIEAGDETIDQIVAAIYMGLDARLIGAAKLSTLA
                     HLEDLVGRGLVAADGGVDLEGRYRRDGR"
     gene            complement(9243..10019)
                     /locus_tag="VKU03_05055"
     CDS             complement(9243..10019)
                     /locus_tag="VKU03_05055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1499 domain-containing protein"
                     /protein_id="HLJ70663.1"
                     /translation="MRRLIIEEPYSTAAIWSRRVGLFALAVAAVAVLLSRLGAVDPPG
                     ALAVLGGALILACLAGLLAAAAFVVIWRTGRRGAGLAWAGLIMALLMLAYPAYLTWKA
                     ISLPIINDVSTDLETPPTFMLSAKARLARGNRTPPESSQATREAQRLAYPAVQPIMVD
                     LETNDAYRLALDVAKQSHWRIVDAVAPSVRENVGHIDAIAGSLVMGFPSDIAIRIKPS
                     ATQTRIDIRSVSRVGRHDFGDNARRIQKFAAAIAQAAKER"
BASE COUNT         1697 a         3322 c         3488 g         1628 t
ORIGIN      
        1 ggtctttgtc ctcggccctt gacacgaaac gccccatgtg agcggaggac ccgccatagc
       61 gccgcacaag gatatgatct tgcacgcagc ttgccggcgc tatggcgggt tgacggcgat
      121 cggccggcgt ggcgccgctg aaagattacg tccaagacgc gccataacct cagactttcc
      181 ccggacagcc ctgcgcgcag cggggcgagg ggattcacag ggaggattaa acccgcaccg
      241 gctcctgcgg aaacgagagc tgctcgcgcg cccgccattc gtgcgtgacg taattcagga
      301 tcaccgagcg gcgcaggcct tgaatcggcc gcttttcgaa gccgtgccac gtgttgtccg
      361 ccggcacgaa aatcatcgcg ccgccgcgct tgaacggcga gcggccgtaa tggcgctcct
      421 tgctcctata gatgtcggtg ccgagctcgt cgtggcctgg cccttgcgac agatagatca
      481 ggcaggtgaa cttcttgacg ccgagatcgg tatgcggctc gagccagaac ccatcgacgt
      541 cctgagcata ttcgagacgc aggaacgtat cgtcgatggg cgcgccgaac gcgtcatgga
      601 tgaggcgcac gactcgccgc gattggagcg cctcggcgac ggcgcgcatc gccggaaagc
      661 gcgccatcgc cgcggcgtcg aaatagctgc gctggtcgtt gtgcaattcg cgcttgccgg
      721 acacgccgtc gaggcgcggc gcggcgaaag gcagatcggc gagcgcgtcg agaatgtcgg
      781 cggggaagac attctcgatg agataatgac gataaggctc gtcgaacgcc cggcgcgccg
      841 cgatcgaggc ggcgaaagcg tcggcgacgc gctgcgcgtc ggggctcaga gtgtcgacag
      901 gcgcggcgcg cggcgcggat tgtgcggaca tttaagctca cccataacgt tttaacttgc
      961 gcggcaatct agttggctgc ggcgcaatgg gcaagtacgc tgggaaatca gtagattaga
     1021 acgggtctga gctggtttcc gcgcttgccg cgatcatttc aagcctcggc ggcgcgcgaa
     1081 cgcgtcgtac cttggcgcct ttcctcccct gcgaagcgaa gcaggggagg ggaggggaac
     1141 catgcgaagc atggtggagg gggcttcgga gattgaggat aatcctgaga gaccgaagcc
     1201 ccctccaccg cgcttgcgcg cggtccccct cccccgctgc gcgggggagg attctcacac
     1261 catgccgagc gcagcgagat agaggtcgag aatttcctgc tcctcgcgtc tcttatccct
     1321 gtcctggcgc cgcatcgaga cgatcttgcg caggattttc acgtcgaacc cattgccctt
     1381 ggcttcggcg taaacgtcct tgatatcgtc cgacagagcc ttcttctctt cttcgagttt
     1441 ttcgacacgc tcgatgaaag agcgcagatg cccggcattg acgacgtcgc ttttcatgac
     1501 tcgcgcccga tgatgtgttc ttatgcgatg cggcgcgaag gcatggctcc gctcgccttc
     1561 tccgtcaagc gcggcggagc cttcatcccc gctgttcttt gcgccggcgc gccttgccgg
     1621 cggcccggct gccgaaatcg atccgttcat ggatattcgg gagggcgagg atcggcgcga
     1681 tcagccgggc gagccgagcc gcccaagcct cggcgtccgg ctcggtcgcg atcagatcct
     1741 ggcggatttc gatcaaagca ttggcgaggc cgcgcgccgt cgcaaccgcg tcgatcgtgt
     1801 cgccggccag ggcgccgtca taaggctcgt tatcgccgac cgcctcgtcc gccggctgca
     1861 gatcgggctc cctcgccagc gcctgcaaca aaggctcggg caggcgcggg tccatgtccc
     1921 acaacacgcc gaccttccaa gggcgcggcc ggccgcgcca agcgggcgtg aaggaatgaa
     1981 ccgagagcac ggccggcccc cggccgctcg cgctcatctg ctcgacgagg tcgacgcaag
     2041 cctgacggta aggccgccaa tagagatcgc gccggagcgc gatctcttcg gcgccgacgc
     2101 gcgcattgcc gggcacaatc gcgccgtcgg aaatctgcat caccagagtc ggatcgtcgg
     2161 cgccccgatt gggatcgatc agcagccggg aaaaggtcga gagcacggcc ggcgcgttga
     2221 gcagccgggc gaggcgccgc gtcagccagg cagcgccgat gtcgtaagcg atatggcggg
     2281 cgaaggcgct cggcggcagg ccgagatcgc cataggccgg cggcagggcg ttcgacgcgt
     2341 gatcgcaaag gatgatgacg ccggcatcga gcgcgccttc gatgaattcg accggctgaa
     2401 aagcgccccc ggccgatgcg gttccggccg ccccagaaag atccatgatc gccgtagtcc
     2461 ttgctcgcgc gatgcggcca gaataaggac tgatcgcgcg atccgccaag ccggcgcttc
     2521 gccgcccgcg ccgccttcgg cgcatcgagc cgaagagctt catgaacagc gcgattaccg
     2581 acgctcaacc cgccaagagt tacggcgcgc cgtttctggc gttggtcgcc ggcgccttgg
     2641 cgatgggcgc ttcgccgatt ttcgtgcgtc tcgccgatgt cgggcctttc accagcgcct
     2701 tctggcgcgt cgcgctcgct ctgccgatcc tctatgcctg gatgcgcgtc gccgagcgcg
     2761 acggcgggcg cagccgcagc ttttcgcgcg cgatcgtcct cagcggcctc gctttcgcgg
     2821 gcgatctctt tttctggcat ttgtcgatcg tctcgacgac cgtcgccaat gcaacgtttt
     2881 tcgcgacgac ggcgccgatc tgggtggtca ttttcggctg gctgctgttt cggacgcgcg
     2941 tctcggcggg cgtgctggcg gggctcgctc tctgtctggc gggcggcggc gcgcttctgg
     3001 cgcagagcct caacctgcat gccggccgcg ccttcggcga cctttgcggc ctgaccacag
     3061 gcgtgttctt cggcctttat tttctcgccg tccaggcggc gcggcgcgag tcttctgcgg
     3121 cgcgcgtcac gttcgaggcg acgctgatca ccgccgccct tctcttgatc gtcgcgctcg
     3181 tgctggagcg gcgcttctgg ccgcacagcc ttccggggct ggccgctctc ctcgccatgg
     3241 cctggatcag tcacgctggc ggccagggcc tgctcgcggc ggcgctcggc cgtttgccgg
     3301 cggcgttctc gtcgctggtc atctttctcg aagcgatcgc cgcggcctgt ttcgcctggg
     3361 ccgcgctcaa tgagccggtc agcgccgttc aagcggcggg cggtctcgcc attcttctgg
     3421 gcatctatgt ggcgcggccg tgaccgaggc ggctgcgtcc tcgccgcgtg ttgacacgcc
     3481 gcctgccgcg tcgaatatgg cgcgccatga cggagcagac gggtgagtct cgggaaaaag
     3541 cgcgcaaaag catcggcctc cttgcttgct cgatcgcgat gctcttcgcc cttgcgcccg
     3601 cccgcgccga tttccgcatg tgcaacaaca cgtcgaaccg ggtcagcgtc gccctcgcct
     3661 ataccgacgg ccagaactgg accagcgaag gctggtggaa cctcaagccg tccgattgcg
     3721 atatcttgct gcgcggcacg ctggcggccg agttctatta cgtctatgcg atggacgaac
     3781 gcggcggcga gtggaagggc aaggccttca tgtgcaccag cgaccgcgaa ttcaagatcg
     3841 acggccggca ggattgctac gtgcgcggct acgatcgtac cgggtttttc gaggtcgata
     3901 caggcaaaga cgcgcgcgac tggacggtgc agcttaccga tccgacctcc ccgtgaccgc
     3961 ctcttcgcga ggggcgctga cgtgacctgc tcatccgccg gacgcggcgc tcaaggacgg
     4021 attcgatgag gcgaaggcgg cgcgttaaga tcattgcgac tttggggccg gcctccctcg
     4081 accgcgcgac cgtcgcctcg ctgttcgagg cgggcgccga cgtcttccgc atcaatatga
     4141 gccatgcgac gcatgacggg atgcgggcgc gcgtcgcgat gatccgctcg ctcgaaaagg
     4201 agtatggccg cccgatcggc attctgatcg atctgcaagg gccgaagctc agggtcggcg
     4261 aattcgagac cggcgccgtc ctcttgcgca agggcgaggc gttcacgctc gattccgatc
     4321 cgacgccagg cgatcaaacg cgcgtctgcc tgccgcatcc ggaaattctc gattcgctcg
     4381 cgcccggcca tcgcctgctg atcgacgacg gcaaggttct gttgaaagtg accagcgtcg
     4441 cgccgggcaa ggcggcgacg attgtcgaag tcgccggccg cgtctcgaac aagaagggcg
     4501 tcagcctgcc cgatacgacc attccgacct cggcgatgac cgagaaggac cgcgaggatc
     4561 tcgacgccgc cctgcaagag gacatcgatt ggatcgcgct ctccttcgtg cagcgtccgg
     4621 aagacgtcgc cgagctccgg atgatcgtcg gcggtcgcgc catggtgatg gccaagatcg
     4681 agaagccgca ggcgatcgcg cggctcgacg agatcatgga ggtctcggac gcgttgatgg
     4741 tggcgcgcgg cgatctcggc gtcgagatgc cgctcgaaca agtgccggga ttgcaaaagc
     4801 gcatcacccg cacggcgcgc cggctcggca agccggtggt ggtggcgacg cagatgctcg
     4861 aatcgatgat ctcctcgccg gtgccgaccc gcgccgaggt ctcggacgtg gcgacggcgg
     4921 tgttcgaagg cgccgacgcg gtgatgctgt cggcggaaag cgcctccggc caatatccgc
     4981 gcgaagcggt ggcgacaatg agccgcatcg ccgagcagat cgagtcggag gaaatctatc
     5041 cggcggtgat caccgcgcag cgcgccagtc ccgagcgcac cggcgccgac gcgatcgcgg
     5101 tggcgacgcg cgatgtcgcc gagacgttgg acttaaagct gatctgcgcc tggacggcgt
     5161 cgggctcgac cggcctgcgc attgcgcgcg agcggccgcg ctcctcgatc gtcgcgctga
     5221 cgccgaatat ctcggcggcg cgccgcctcg ctctggtctg gggcgtgcat gcgatcgtca
     5281 ccgaagacgc gcgcgacgag cgcgacatgg ccgagcgcgc ctgccgcctc gcggcgaccg
     5341 aaggcttcgc cgaggtcggc gagcgcatca tcatcgtcgc cggcgtgccc ttcggcacgc
     5401 cgggcgcgac caacaatatg cgcatcgcct atgtcgaggc gcacgacgcc gcggaatgaa
     5461 gcgagggacg tacccttctc ccgccttgcg gggagaaggc gccgtcaggc gcggctttta
     5521 gttcttggcg gcaaaaacgc catcgccgag cggaggagtc aaggttcgcg gagcgaagcg
     5581 tagcgacgcg cgaagcgcgc tcggccttga cgccgagcac ggcgatggcg tgcaggataa
     5641 ggcgccgggg ccgccgctgt catcgcgagg agcgcagcga cgaagcatgc attcgtcggg
     5701 ctatgagcgg gctgcttcgc ttcgctcgca acacgacgcg cggctagcgg aacgagggta
     5761 cgtcacagat cctcgccctg gacggccttg gtcaggcgct cgacgctgtc cttcagcgac
     5821 ttgagctgcg gcgcgatctg gagcgcccgc tgataggcgc gcagggcgtc gtcgcgaaat
     5881 ccttcgcgtt cgagaatgac gcccatgccg gcaagcgcgc cgatatgccg cggctcgagc
     5941 ttgaggacat gggcgatgtc ggccatcgag ccgcgatcgt cgtcgacgag gaagcgcacc
     6001 gtcgcgcgct tgttccaggc ctcggcccat tccggatgaa ggtcgacaag cttgtcgagc
     6061 agcgccagcg cgacgtcgta attcttggcg gccatcgcgg cgacggcgcg cgccatcaga
     6121 agatcgccgg cgtcggagcc ggattcgagc tcgagccggt cgatcgcgcc gatcaggccg
     6181 gcggtctcgt cggcgtcttt cgaagcggcg aggcgggcat aaaggtcatt gagcaactcg
     6241 gcgcgggtgc gcggcgccgg cggcttctct tgcgtcgaat cctcgggctt cgacgatcga
     6301 ggcgccgcat tctcgcttcc cgggctcacg ccatgggcgc gcggcggcag gggaaccggc
     6361 gacaggcctg gcgcgcgaag cgctggccga ccctcttcgg gggcggcggc cgacgcgcca
     6421 agcgccgccg ccagcagagc gaccgcgacg atcgaaccca ggcggatcat ccgccaagac
     6481 taggcgtcgg catgaacagg gtcaaaagcc ttccgccgcc gcgccgcggc ggagccgtca
     6541 cattcgagtg agagggcgac aagccgccca gcgatcaacc ctggcgcgcc ttgaagcgct
     6601 tctgcgtctt gttgatgatg tagacgcgac ccttgcgccg cacgagttga ttgtcgcggt
     6661 ggcgcttgcg cagcgatttc aaggaattac ggactttcat gactcaccgg tgaccaaaac
     6721 gccgccggga gcgacccagg gctgaaatat tcggaggcgg tgctatgcct catctctgcg
     6781 tggcgatcaa ggttgaaacg ccaaaggccg ccggcgcggc aaaacctcag cggatcctca
     6841 ggcgctccat cagccgcctg tgcccgtccg gaatcggcgt cggcttccgg ctggcgcgct
     6901 cgacatagac atgggtgtag cggccctggg cggcggcctt ttcggcgccc gctttgaaga
     6961 ccgccagcag ataacgcacc gacgagcggc cgatctcttc gaccgccacc ccaacctcga
     7021 tcggatcggg aaaggccagg ctcgaaaaga aggcgcaatt gctgtcgacg acgaggccga
     7081 cgatcgggct ctcggcggga tcgagcaggc cggcttcgac cagcagggcg ttcaccgcgg
     7141 tgtcgaagaa cgaataatag gcgacgttgt tgacatggcc atagacgtcg ttgtccgacc
     7201 agcgggtcgc gatcggcacg aaacgggcga aagcggcgcg cccgagcggc gagggccgcg
     7261 gcgctttcgc gtcaggcatc ggtcaggcgc ggctgcgcag gccgagcgcg gcgctccaga
     7321 aggcgccgac caacgcccag gcgagcagcg tcttgaccag ggtcgcggcg aaaaacggcg
     7381 ccacgcccgc ggcccaagcc ttttccgcgc cgatcaggcc ggcgagccac aaaaagccga
     7441 acgcgaaaat cagcgcatgg ccgagcgcca tcgccgcgag caggcgcggc ccggaccggt
     7501 cccagccgcg ctcggcgaga tagccgatgg cgaccgccgc ggcgaggaag ccggccagat
     7561 agccgccggt cggtcccgcc atataagtcg ggccggccga ggcgccggcg aagaccggca
     7621 ggccgatcac gccttcggcc aggtagagcg ccaccgtcgc gccggcgagt ctcgcgccga
     7681 aagccgcgcc gaggatcagc acgacgagcg tctgcaaggt catcggcacg ggatagaagg
     7741 gaacctgcac tttggcggac agcgtcagcg cgagcgttcc gatcaccgcc aaagcggcga
     7801 cgcccgcccg cgaggcggcg cgctcgggcc agaggaaagc ggcgagagtc gggcggcgat
     7861 tgtcgttcat ggattcccct tcggcgcgct ttcaacgcga cgcgatatcg cgcttggttg
     7921 cagtcgtgcc tatagaggag gcggccggga tgaacaagcg cgcaaccctg tagccgcggt
     7981 cgtcgaccgc ggccacaatt gcagcgccct ggccgcggtc gtcgaccgag gctgcaagcg
     8041 ctcgcagaac gaggatgaag gatgagcggt gacgacgatc tgattttcga tgcggaggac
     8101 gccgaggccg gcgtcgtcaa gcgcgtctcg ccgctcgtgc gccggctgat cgccggcaat
     8161 ccctcgcctt tcaccttcac cggcacctgt tcgtacattg tcggccaagg ctcggtcgcg
     8221 attgtcgatc cgggcccgct cgacgaggcg catatcgcgg cgctgctcgc cgccatgcgc
     8281 ggcgaaagcg tgcggcatat tctcgtcacc cacacccatc gcgaccattc gcccgccgcg
     8341 gcgcggctca aggcggagac cggcgcggcg attgtcggcg cgcggccttt cgcgccaaag
     8401 aggggcgccg gggaaggcaa gggcctcgac gcggcgcacg atctcgccta cgcgccggac
     8461 cacgcgctcg gcgacggcga gcgcgtcgcc ggagaaggct atacgctcga agccgtggcg
     8521 acgcccggcc acgccgccaa ccatctctgc ttcgccctca tcgaggagaa cagcctgttc
     8581 tccggcgatc acgtcatggc ctggtcgacc accgtggtcg cgccgcccga cggctcgatg
     8641 agcgactaca tggcctcgct cgaaaagttg cgggcgcgcg gcgagaccct gttctggcct
     8701 ggccatggcg gaccggtgcg caacccgcag cgctacctgc gcggcctcgc ccatcaccgc
     8761 cgccagcgcg aggcggcgat cctcgcccga atcgaagcgg gcgacgagac gatcgatcag
     8821 attgtcgcgg cgatctatat ggggctggac gcgcggctga tcggcgcggc gaagctgtcg
     8881 acgctggcgc atctggagga tttggtcggg cggggattgg ttgcggccga cgggggcgtg
     8941 gatttggagg ggcgataccg gcgcgacgga agatgacgcc gtcattgcga ggagcgtagc
     9001 gacgaagcaa tccattcgga gcgcgccgtc gtacgaatgg atacgccatg gccgtgctcg
     9061 acgatggcgt tgtcgttacc aaaacgcctt gatagcctat agagccaacc ctcgcgcctc
     9121 tccggcgcat gtactttcgc gggagcgttc gtggcgctat ggcgggttgg cggcgatcgc
     9181 ccggcgcggc cctgcggcgc ccttgtcctc cccgtgaagc gaagcgaaac gggcgaggaa
     9241 agctaccgct ccttcgcggc ctgcgcgatc gccgcggcga atttctggat gcgccttgca
     9301 ttgtcgccga aatcgtggcg gccgacgcgc gagacggagc ggatgtcgat gcgcgtctgc
     9361 gtcgccgagg gcttgatgcg gatggcgatg tcggagggaa agcccatcac cagcgagccg
     9421 gctatggcgt cgatatggcc gacattctcg cgcacgctcg gcgccaccgc gtcgacaatg
     9481 cgccaatggc tctgcttggc gacgtcgagc gccaggcgat aggcgtcgtt ggtctcgaga
     9541 tcgaccatga tcggctggac cgcgggatag gcgagccgct gcgcctcccg ggtcgcctga
     9601 gaggattcag gcggcgtgcg gttgccgcgc gccagccgcg ccttggcgga tagcatgaag
     9661 gtcggcggcg tttcgagatc ggtcgagacg tcgttgatga tcggcagcga aatcgccttc
     9721 caggtcagat aggccggata ggcgagcatc agcagcgcca tgatgaggcc ggcccaggcg
     9781 aggccggcgc cgcgccggcc ggtgcgccag atcaccacga aggccgccgc cgcaagcagg
     9841 ccggcgaggc aggcgagaat cagcgcgccg ccgagcacgg cgagcgcccc gggcggatca
     9901 accgcgccga gccgcgacaa aagcaccgcg acggccgcca ccgccaaagc gaagagaccg
     9961 acgcggcggc tccagatcgc ggctgtcgaa taaggctctt cgatgatcag gcggcgcatg
    10021 acgccgaggt tctaagggcg agatcaggcg cggtccactc cgccgccgcg gcggaccagg
    10081 gcgacgtttg gcctcaccgc attgacaaac gctccggcgg ctatgtgaat cgctg
//
LOCUS       DATGJW010000166         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_963943, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000166 DATGJW010000000
VERSION     DATGJW010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_963943"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..246
                     /locus_tag="VKU03_05060"
     CDS             <1..246
                     /locus_tag="VKU03_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70664.1"
                     /translation="SADSEALMITMGATTPRLRRGVCGKTATVSAALCGGAPAEDAVE
                     GAYLHSDSRLASQPARRQENRRNVASPLAPPPRGTMK"
     gene            295..2382
                     /locus_tag="VKU03_05065"
     CDS             295..2382
                     /locus_tag="VKU03_05065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin oxidoreductase family protein"
                     /protein_id="HLJ70665.1"
                     /translation="MNAPALVARRPSVCPHDCPSACALDVEVIDGSRIGRVHGAEDQT
                     YTAGVVCAKVARYAERIHHKDRLLQPLKRTGPKGSKQFAPISWDDALDIVAENFLKCE
                     REFGSESVWPYHYAGTMGLLMRDGVRRLTHAKRYSGFFSTICINTAWPGFIAGTGKLT
                     GPDPREMAKADCVVIWGTNAVATQVNVMTHATRARKERGAKIVVVDIYRNETMKQADL
                     GLCLRPGTDGALACAVMHVLFRDNLADRAYLERYTDAPGELEAHLKTRGPEWASAITG
                     LSVAEIEAFAHLVGERKRTYFRLGYGFSRQRNGAANMHAATCIAAVTGAWLHEGGGAF
                     HNNGGLYHWRKTLIEGLDVVDPEIRVLDQSRIGAVLCGEDEALRGRGPVKALLVQSTN
                     PATVAPNQEKVRRGLHRDDLFICVHEHFLTETARFADIVLPATMFLEHDDLYQGGGHS
                     YVMFGPKAIEPAGQCRSNHDLVCALAKKLGAEHRGFQMSPREIIDWTLQASGWGTLAE
                     LEEKKWLDVQPPFEKSHFLRGFGFKDKRFRFKADWAATPFANIGLMGPWRAMPSLPDH
                     WAIIEEVDDEHPFRLATSPSRGFLNTTFTETPTSRQRESRPNVMIHPDDAARLNVADG
                     DIVRLGNKRGETRLHAKLFPGVRPGVLISEGVWPAEAFLDGKGINVLTGDDAVAPFGG
                     AAFHDNRVWLRRA"
     gene            2453..2528
                     /locus_tag="VKU03_05070"
     tRNA            2453..2528
                     /locus_tag="VKU03_05070"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2487..2489,aa:Glu,seq:ttc)
BASE COUNT          460 a          931 c          855 g          443 t
ORIGIN      
        1 tcggcggatt ccgaggcgct gatgatcacg atgggcgcga cgacgccccg cttgcgccga
       61 ggcgtttgtg gcaaaaccgc cacagtttca gccgcccttt gtggcggcgc gcccgctgaa
      121 gacgccgtcg agggcgccta tctccacagc gattcgagat tggcgtcgca gccggcgcgg
      181 cggcaggaga atcggcgcaa tgtcgcttcg cctcttgcgc cgcctccccg cggcactatg
      241 aaatagccaa agcatggctc agacgatttt ccgcgacaca tcgaggcggc ggcgatgaac
      301 gcgcccgctc tcgtcgcgcg ccgcccctcg gtctgcccgc atgactgccc ctcggcctgc
      361 gcgctcgatg tcgaggtgat cgatggctcg cgcatcggcc gggtgcacgg cgccgaggat
      421 cagacctaca cggcgggcgt ggtctgcgcg aaggtcgcgc gctatgccga gcgcatccat
      481 cacaaggacc ggctgctcca gccgctgaag cggaccgggc cgaagggctc caaacaattc
      541 gcgccgatct cgtgggacga cgcgctcgac atcgtcgccg agaatttcct caaatgcgag
      601 cgcgaattcg gctcggaaag cgtctggccg taccattacg ccggcacgat gggccttctc
      661 atgcgcgacg gcgtgcgccg cctgacccac gccaagcgct attccggctt cttctcgacg
      721 atctgcatca acaccgcctg gcccggcttc atcgccggga ccggcaagct gaccggcccc
      781 gatccgcgcg aaatggccaa ggccgattgc gtcgtgatct ggggcaccaa cgcggtcgcg
      841 acgcaggtca atgtgatgac ccatgcgacc cgcgcccgaa aggagcgcgg cgccaagatc
      901 gtcgtcgtcg acatctatcg caacgagacg atgaagcagg ccgatctcgg cctgtgtctc
      961 aggcccggca cggacggcgc gctcgcctgc gcggtgatgc atgtcctgtt ccgcgacaat
     1021 ctcgccgacc gcgcctatct tgagcgctat accgacgcgc cgggcgagct ggaggcgcat
     1081 ctgaaaacgc gcggtcccga atgggcgagc gcgatcaccg gcctatccgt cgccgagatc
     1141 gaggccttcg cccatctggt gggggagcgc aagcgtacgt atttccggct cggctacggc
     1201 ttctcgcgcc agcgcaacgg cgcggccaat atgcacgcgg cgacgtgcat cgccgccgtc
     1261 accggcgcct ggcttcatga aggcggcggc gcctttcaca acaatggcgg cctctatcat
     1321 tggcgcaaga cgctgatcga ggggctcgac gtcgtcgatc cggaaatccg cgtcctcgac
     1381 cagtcgcgca tcggcgcggt tctctgcggc gaggacgagg cgctgcgcgg ccgcggcccg
     1441 gtcaaggcgc tgctcgtcca gagcaccaac cctgcgaccg tcgcgccgaa ccaggagaag
     1501 gtgcgccgcg gcctgcatcg cgacgatctc ttcatctgcg tgcacgagca tttcctgacc
     1561 gagacggcgc gcttcgccga catcgtgctg ccggcgacga tgttcctcga acacgacgat
     1621 ctctatcagg gcggcggcca ttcctacgtg atgttcggcc ccaaagcgat cgagccggcc
     1681 ggccaatgcc gctcgaatca cgacctcgtc tgcgctttgg ccaagaagct cggcgccgag
     1741 catcgcggct ttcagatgag cccgcgcgag atcatcgact ggacgctgca agcctccggc
     1801 tggggcacgc tggccgagct cgaagagaag aaatggctcg acgtgcagcc gcccttcgag
     1861 aaatcgcatt tcctccgcgg cttcggcttc aaggacaagc gcttccgctt caaggccgat
     1921 tgggccgcaa cccccttcgc caatatcggc ctgatggggc cgtggcgcgc catgccgtcc
     1981 ctgcccgatc attgggcgat catcgaggag gtggacgatg agcacccgtt ccgcctggcg
     2041 acatcgccct cgcgcggctt tctcaatacg accttcaccg agacgccgac ttcgcgccag
     2101 cgcgagagcc ggccgaatgt gatgatccat cccgacgacg ccgcgcgcct taacgtcgcc
     2161 gacggcgaca tcgtgcggct cggcaacaag cgcggcgaga cgcggctgca cgcgaaactc
     2221 ttcccgggcg tgagacccgg cgtgctgatc agcgagggcg tctggccggc cgaggccttc
     2281 ctcgacggca aggggatcaa cgtgctgacc ggcgacgacg cggtcgcgcc gttcggcggc
     2341 gcggctttcc acgacaaccg cgtctggctg cgccgcgctt aaagcccggc cttgccgggg
     2401 cgcccgcttg cctctatagg ggtcgcgcct cggccaagcc gaaagcccgc aagcgcccat
     2461 cgtctagcgg tccaggacgc cgccctttca cggcggaaac acgggttcga ttcccgttgg
     2521 gcgcgccaat aatttcaaag acttagcaaa aacgcctccc atttgtccaa aaatctgtcc
     2581 gctatacgag cgtggacgag catggatttt cgtggcgtga ggaaggcgcg gccctgcgcc
     2641 ggtctcagtt cggcctaaat aacgccttgt ggctggccaa gcttccgcc
//
LOCUS       DATGJW010000167         2033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1481983, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000167 DATGJW010000000
VERSION     DATGJW010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2033
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1481983"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..1107)
                     /gene="nadC"
                     /locus_tag="VKU03_05075"
     CDS             complement(253..1107)
                     /gene="nadC"
                     /locus_tag="VKU03_05075"
                     /EC_number="2.4.2.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012044766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004514 - nicotinate-nucleotide
                     diphosphorylase (carboxylating) activity [Evidence IEA];
                     GO_process: GO:0019363 - pyridine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylating nicotinate-nucleotide
                     diphosphorylase"
                     /protein_id="HLJ70666.1"
                     /translation="MALVPPPTLLIEPIVREALLEDLGRAGDITTDAIAPPDCAARTV
                     MAARQEGVVAGLDLAALAFRLIDPAIRFKPIMADGATVMPGAAIAAIEGPARGILSAE
                     RVALNFTSRLSGIASATAKLSAAVAHTQTRIVCTRKTTPLLRGIEKYAVRAGGGVNHR
                     FGLDDAALIKDNHVAIAGGVAEAIRRVRAHVGHLVKIEVEVDTLAELEEALQAGVDAV
                     LLDNMAIGELRKGVAMIGGRAISEASGRVTLETAPAIAETGVDLISVGWLTHSAPILD
                     IGLDYDRR"
     gene            complement(1112..>2033)
                     /locus_tag="VKU03_05080"
     CDS             complement(1112..>2033)
                     /locus_tag="VKU03_05080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLJ70667.1"
                     /translation="SFGQGLALAARAGAVLADMEFVQFHPTALDVRTRPAPLISEAVR
                     GEGAILIDDAGDRFMVETPGAELAPRDVVARAIWRRRAEGRRVFLDARRALGDRFAAR
                     FPAIAAACRAAGIDPAREPIPVRPAQHYHMGGVAVDAEGRASVVGLWACGEVASTGLH
                     GANRLASNSLAEAAVFAAAVARSIEGAPASGAAPIRNFDRTPAASVPDAVRPVLSRAA
                     GVIRDRERLASAIGALAALARFGSAAADPALVGLMIAVAAFRREHSLGAHCRADFPGR
                     PAAPTRSRLTLAEAFAEAEAIAPCPLARQA"
BASE COUNT          315 a          719 c          722 g          277 t
ORIGIN      
        1 agagttcctc ggccatgttc gcaagtgttc tgaccggttg ccgcgaacgc aagcccacca
       61 tagcccagga atatgcgcat gcaagcaacc gcaggacgcg tgccgggcat ccgccgcggc
      121 gcccttccgg cctaagctca aggattgcgc ggggcagccg ccgaccgagg cttcgcgccg
      181 ctcgccggat atatgcgtcc ggttgcgaat ccgcggagac cggcgcgagc cccgcgcctg
      241 ccgacaagct agctaacgcc ggtcgtagtc gagcccgatg tcgaggatcg gcgcgctgtg
      301 ggtgagccag ccgaccgata tgagatcgac tccggtttcg gcgatcgccg gcgcggtttc
      361 gagggtcacg cggcccgagg cctcgctgat cgcccgaccg ccaatcatcg cgacgccctt
      421 tcgcaattcg ccgattgcca tgttgtcgag caacacggcg tcgactccgg cttgaagcgc
      481 ctcttcgagt tcggcaagcg tatccacctc gacctcgatc ttgacgaggt ggccaacatg
      541 agcgcgcacg cggcggatcg cctccgcaac gccgccggcg atggcgacgt gattgtcctt
      601 gatcagagcc gcatcgtcga ggccgaagcg gtggtttacc ccaccccccg cgcgcaccgc
      661 gtatttctcg attccgcgca ggagcggcgt cgtcttgcgg gtgcagacga tgcgcgtttg
      721 tgtgtgcgcg accgccgcgg acagttttgc ggtcgcgctc gcgatcccgc tcagccggct
      781 tgtgaagttg agcgcgacgc gctcggccga caggatgccg cgcgcgggcc cctcgattgc
      841 ggcgatcgcc gctcccggca tgacggttgc gccgtcggcc atgatcggct tgaaccggat
      901 cgcaggatcg atgagccgaa acgcgagggc cgcgagatcg aggccggcga ccacgccttc
      961 ctggcgcgcc gccatcacgg tccgggcggc gcaatccggc ggggcgatgg cgtcggtcgt
     1021 gatgtcgccg gcgcggccga ggtcctcgag cagcgcctca cggacgatcg gctcaatgag
     1081 gagggtgggc ggcggaacga gcgccatgac ctcaagcctg gcgcgcgaga ggacaaggcg
     1141 cgatggcctc cgcctcggcg aaagcttcgg ccagggtcag gcgggagcgg gttggcgcgg
     1201 cgggtcgccc gggaaaatcg gcacggcaat gggcgcccag gctgtgctcg cggcggaacg
     1261 ccgcgacggc gatcatcagc ccgaccagcg cgggatcggc ggccgccgat ccgaaccggg
     1321 ccaaggcggc gagcgcgccg atcgcggatg cgagccgctc gcggtcgcgg atgacgcccg
     1381 ccgcccgcga gaggaccggc cgcacggcat cgggaacgga ggccgcgggc gtccggtcga
     1441 agtttcggat cggcgcggcg ccgctcgccg gcgcaccttc gatggagcgg gcgaccgccg
     1501 ccgcgaacac cgcagcctcg gcgagagagt tgctcgcgag ccggtttgcc ccgtgcaggc
     1561 cggttgaggc gacttccccg caggcccaga gaccgacgac cgacgcccgc ccctcagcgt
     1621 ccacggcgac gccgcccatg tgatagtgct gcgcgggccg gacagggatc ggctcgcgcg
     1681 ccggatcaat gccggccgcc cggcaagccg ccgcaatggc cgggaagcgc gcggcgaagc
     1741 ggtcgccaag cgcgcgccgc gcgtcgagaa aaacgcgcct gccttcggct cgcctccgcc
     1801 agatcgcccg agcgacaacg tctcgcggcg caagctcggc tcccggcgtc tcaaccatga
     1861 acctgtcgcc ggcgtcgtca atcaggatcg cgccttcgcc gcgcaccgcc tcgctgatga
     1921 gcggcgccgg ccgtgtcctc acgtcgaggg cggtcgggtg gaactggacg aactccatat
     1981 cggccaggac cgcgccggcg cgcgcggcaa gcgccagacc ctgcccaaaa ctg
//
LOCUS       DATGJW010000168         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_861582, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000168 DATGJW010000000
VERSION     DATGJW010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_861582"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VKU03_05085"
     CDS             complement(<1..276)
                     /locus_tag="VKU03_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824746.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyric acid synthase CobQ"
                     /protein_id="HLJ70668.1"
                     /translation="MARRLMFQGTGSDVGKSLIVAGLARAFTRRGLRVAPFKPQNMSN
                     NAAVTADGGEIGRAQALQARAARRAPTVHMNPVLLKPQSEIGAQVVVH"
     gene            complement(276..887)
                     /gene="cobO"
                     /locus_tag="VKU03_05090"
     CDS             complement(276..887)
                     /gene="cobO"
                     /locus_tag="VKU03_05090"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928812.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cob(I)yrinic acid a,c-diamide
                     adenosyltransferase"
                     /protein_id="HLJ70669.1"
                     /translation="MQIVDEAERHKQKMQKRKEAQDREVASKIVEKGLLIVNTGKGKG
                     KSTAAFGLILRALGHGWRVGVVQFIKGAWSTGERAVLETRFADLVSWHTMGEGFTWET
                     QDRARDVAAAQAAWAKAQALMDDETIRLVVLDELNIALRYDYLPADAVVETLVKRRPD
                     LHVVVTGRNAPPALIEAADLVTEMNAVKHHFAAGVKAQAGIEF"
     gene            complement(996..>2582)
                     /gene="polA"
                     /locus_tag="VKU03_05095"
     CDS             complement(996..>2582)
                     /gene="polA"
                     /locus_tag="VKU03_05095"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018964141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006261 - DNA-templated DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase I"
                     /protein_id="HLJ70670.1"
                     /translation="KPLFEAPDVIKIGHNVKFDWVMLAERGVEVAPIDDTMLISYALD
                     AAATTDGHGMDWLAERFLGHQTIKFSDVAGSGKSFIGFARVPIDKACEYAAEDVDVTL
                     RLWRALKSRLPAEHVGKVYETLERPLVVPLARMERRGVSIDPAVLSRLSGEFAQGMAR
                     LEAEIHKLAGEPFNLGSPKQLGDILFGKMGLPGAKKTATGAWSTSASILDDLAEEGHE
                     LPARILEWRQLAKLKSTYTDALPSYVNPRTRRVHTSYALAATTTGRLSSSEPNLQNIP
                     IRNEEGRKIRRAFVADKGYALISADYSQIELRLLAHVADIPQLKQAFADGLDIHAMTA
                     SEMFGVPVKDMPSEIRRRAKAINFGIIYGISAFGLANQLGIPREEAGAYIRKYFERFP
                     GIRAYMEAMKKQARADGYVRTIFGRKCHFPRIAASNPSERAFNERAAINAPLQGSAAD
                     IIRRAMIRMDDALAKAKLNAMMLLQVHDELVFEAPLDEVEATIKLVVKVMEEAPLPAA
                     QLSAPLKVDARAAANWDEAH"
BASE COUNT          423 a          838 c          845 g          476 t
ORIGIN      
        1 gtgcacgacg acctgcgcgc cgatttcgct ttgcggtttc agcaagaccg gattcatgtg
       61 aaccgtcggc gcgcgccgcg ccgcgcgcgc ctgcaaggcc tgggcgcggc cgatctcgcc
      121 gccgtcggcg gtcaccgcgg cgttgttcga catgttctgc ggcttgaacg gcgcgacgcg
      181 gaggccgcgc cgcgtaaaag cccgcgccag cccggcgacg atgagcgact tgccgacgtc
      241 cgagcccgta ccctgaaaca tcaggcggcg ggccatcaga attcgattcc cgcctgcgcc
      301 ttcaccccgg cggcgaaatg atgtttcacc gcgttcattt cggtgacgag gtcggccgcc
      361 tcgatcagcg ccggcggcgc attccggccg gtgacgacaa cgtgcaaatc ggggcggcgc
      421 ttgaccagcg tctcgaccac cgcgtccgcc ggaagatagt cgtagcgcag cgcgatattg
      481 agctcatcga gcacgacgag acggatcgtc tcgtcgtcca tcagcgcctg cgccttggcc
      541 caggcggctt gcgcggcggc gacgtcgcgg gcgcggtcct gcgtctccca ggtgaagcct
      601 tcgcccatcg tatgccagga gacgagatcg gcgaagcgcg tttcgagcac ggcccgctcg
      661 ccggtcgacc acgcgccctt gatgaattgc accacgccca cccgccaacc atggccgagg
      721 gcgcgcagga tgaggccgaa agccgcggtc gacttgccct tgcccttgcc agtattgacg
      781 atcagcagcc ctttttcgac gatcttggag gcgacctcgc gatcctgcgc ctccttgcgc
      841 ttttgcatct tctgcttgtg ccgctcggct tcgtccacga tctgcatcct atctttgctt
      901 cagacggcga aaattccagc gcagccggcg cgtcttcccg tcgagaaatg ctgttggcct
      961 caatccgccg ggaggaccat aaggcttcgc cgccgtcaat gcgcctcgtc ccaattggcg
     1021 gcggcgcggg cgtcgacttt gagcggcgcg gaaagctgcg ccgccggcaa cggcgcctcc
     1081 tccatcacct tgacgacgag cttgatcgtc gcctcgactt cgtcgagcgg cgcctcgaag
     1141 acaagctcgt catggacctg cagcagcatc atcgcgttga gcttcgcctt ggccaaggcg
     1201 tcgtccatgc ggatcatggc gcggcggatg atgtcggcgg ccgagccctg gagcggcgcg
     1261 ttgatcgccg cgcgctcatt gaaggcgcgc tcggaagggt tcgaggcggc gatgcgcggg
     1321 aaatggcatt tgcggccgaa tatggtgcgc acatagccgt cggcgcgcgc ctgtttcttc
     1381 atcgcctcca tataggcgcg gatgccgggg aagcgctcga aatactttct gatgtaagcg
     1441 ccggcctctt cgcgcggaat gccgagttgg ttggcgaggc cgaaagcgga aataccatag
     1501 atgatgccga agttgatcgc cttggcgcgc cgccggatct cgctcggcat gtccttcacc
     1561 ggcacgccga acatttcgga cgcggtcatc gcgtggatgt cgagcccgtc agcgaaggcc
     1621 tgcttcaatt gcgggatgtc ggcgacatgg gcgagcaacc gcaattcgat ctggctgtaa
     1681 tcggccgaga tcagcgcgta gcccttatcg gcgacgaagg cgcggcgaat cttgcgcccc
     1741 tcctcgttgc ggatcggaat gttctgcaag ttcggctcgg aggaggagag gcggccggtc
     1801 gtcgtcgctg cgagagcgta gctggtgtga acgcggcggg tgcgcgggtt gacatagctc
     1861 ggcagcgcat cggtatacgt cgatttcagt ttcgcgagct ggcgccattc gagaatgcgc
     1921 gccggcaatt catgtccctc ctcggcgaga tcgtcgagga tgctggcgga tgtcgaccag
     1981 gccccggtcg cagtcttctt cgcgcccggc aggcccatct tgccaaacag gatgtcgccg
     2041 agctgcttgg gcgagccgag attgaacggc tcgccggcga gcttgtggat ttcggcctcg
     2101 agccgcgcca tgccttgcgc aaattcgcca gaaaggcgcg acagcacggc cggatcgatc
     2161 gaaacgccgc gccgctccat ccgcgccaga ggtacgacga gcggccgctc cagcgtctcg
     2221 taaactttgc cgacatgctc ggcgggcagc cggcttttga gcgcgcgcca aagccggagc
     2281 gtcacgtcga catcctcggc ggcgtattcg cacgccttgt cgatcggcac gcgggcgaag
     2341 ccgatgaagc tcttgcccga gccggccacg tccgagaatt ttatcgtctg atggccaaga
     2401 aagcgctcgg cgagccaatc catgccgtgc ccgtcggtcg tcgcggcggc gtcgagcgcg
     2461 taggagatca gcatcgtatc gtcgatcggc gcgacctcga cgccgcgctc ggcgagcatg
     2521 acccaatcga atttgacgtt gtggccgatc ttgatcacgt cgggcgcttc gaagagcggc
     2581 tt
//
LOCUS       DATGJW010000169         3702 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_238399, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000169 DATGJW010000000
VERSION     DATGJW010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3702)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3702)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3702
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_238399"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1046
                     /locus_tag="VKU03_05100"
     CDS             <1..1046
                     /locus_tag="VKU03_05100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70671.1"
                     /translation="NWTTFTVGGALDVSSSGGTLTIGSATSGGGQTLAALGDLVFTHL
                     TTMGIPGDPGNVDLTSTAGGIRGDAIGANGGVTIDAFGAIAIASLHARGGVNLSSQTA
                     LSLPAVTTGSGSVFDSPIINIGTIRPDAGVVGPFVLGFTGYRGAVGSELNVSVDGAPY
                     VILSELREDNAQIATDAKAFAIDAGYIVDVLGLTTPYENLLMNNQTSRPVIGPDVQLF
                     VPTYSFTLAQNIFLTTTSAFVVRYDGVGEIDDRPPGYGIFGASFVRDFDRQARDGDIP
                     LILTTDEMGLPPSWRHAVWPNPIFFGSPAQSATPGVPAVNLGGALTVGEEIWQLANDN
                     GGLEIVVRRRNRR"
     gene            complement(1119..1445)
                     /locus_tag="VKU03_05105"
     CDS             complement(1119..1445)
                     /locus_tag="VKU03_05105"
                     /inference="COORDINATES: protein motif:HMM:NF025428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4253 domain-containing protein"
                     /protein_id="HLJ70672.1"
                     /translation="MCRACAIFHDGTAKVDRAHCVDPDPRPTTAPAYMSLGNWNACPP
                     PADRVAALRVWRDRYGAEFVGLDANTINLRVSRKPAARDEALELARAHYTFATTSDLI
                     ALRPLD"
     gene            complement(1641..2348)
                     /locus_tag="VKU03_05110"
     CDS             complement(1641..2348)
                     /locus_tag="VKU03_05110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877424.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HLJ70673.1"
                     /translation="MQEPIAISRQKLFEQVAAHIERQILDGALRPGDRLPTERELQAR
                     FGVGRPAIREALITLQRAGLIEISNGAPARVSMPTASDVVAGMMPAVLQMLSTEDGQH
                     HFQRVRMFFECGLARQAAREANEAQVEKLRAALAANKAAIGDRDLFIATDIAFHFVLA
                     EITGNPVFIALHDAMSSWLRQQRTVTLERQGQDRIAYNAHAHIYEAIEARDSDAAEAA
                     MRQHLLQLEDAYWRRGD"
     gene            2498..3451
                     /locus_tag="VKU03_05115"
     CDS             2498..3451
                     /locus_tag="VKU03_05115"
                     /EC_number="1.1.1.169"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008677 - 2-dehydropantoate 2-reductase
                     activity [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase"
                     /protein_id="HLJ70674.1"
                     /translation="MKIAILGGGGAMGGIFGGWLAAAGEDVTLIDVSRAAIEQIRARG
                     LRIDEKDGSSRVVPVAATTAPAEIGPVDLIVNFVKCYHTDAAIRAAQPMLGPDAAVLT
                     LQNGWGNADKIAALVGRERVLVGLTYHGGTLLGPGHVKHPGVGMTYVGELDGSTSPRL
                     QRVVEAFRRAGFETTLSRAILDEVWKKLALNACTLPTAALLRFMAHQLVAHAGVEAEM
                     RSILEEVVAVAKAQGIALDFEERWTAICGLLEKAIGGKASMLQDVEARRQTEIEVING
                     AIVAAGQKHGVATPHNEAMVRLIKSLEEAYLADAAKERAAS"
     gene            3448..>3702
                     /locus_tag="VKU03_05120"
     CDS             3448..>3702
                     /locus_tag="VKU03_05120"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ70675.1"
                     /translation="MTGRLDGKVAIVTGAARGIGAAIVRRFVAEGARAALLDLDRRGG
                     EDVARSLGAAARFFPCDVTRAADCDRAVAAAASLFGAATIL"
BASE COUNT          646 a         1171 c         1228 g          657 t
ORIGIN      
        1 tcaactggac gaccttcacc gtcggcggcg cgctcgacgt ttcgtcgagc ggcggaacgc
       61 tgacgatcgg ctcggcgacg agcggcggcg ggcagacgct cgccgcgctc ggcgacctcg
      121 tcttcacgca cctgacgacg atgggcattc caggcgatcc gggcaatgtc gacttgactt
      181 cgacggcggg cggcattcgc ggcgacgcga tcggcgccaa tggcggcgtc acgatcgacg
      241 ccttcggcgc catcgccatc gcgtcgcttc acgcccgcgg cggcgtcaat ctcagctcgc
      301 aaacggcgct gtcgcttccg gcggtgacga ccggctcggg ctcggtgttc gattcgccga
      361 tcatcaatat cggaaccatc cggcccgacg ccggcgtcgt cggccccttc gtgctcggct
      421 ttaccggcta tcgcggcgcg gtgggctccg agctcaatgt cagcgtcgac ggcgcgccgt
      481 acgtgattct gagcgaattg cgcgaagaca atgcgcagat cgccaccgac gcgaaagcgt
      541 tcgcgatcga cgccggctac atcgtcgatg tgctcggatt gacgactcct tacgagaatt
      601 tgttgatgaa caatcaaacg tccaggccgg tcatcggtcc ggacgtgcag ttgttcgtgc
      661 cgacctacag cttcacgctg gcccagaata ttttcctgac gacgacgagc gcgtttgtcg
      721 tgcgctacga cggcgtcggc gagatcgacg atcggccgcc tggatacggg attttcgggg
      781 cgagtttcgt tcgcgatttc gaccggcagg cgcgcgacgg cgacatcccg ctcatcctaa
      841 cgaccgacga aatgggcctg ccgccgtcct ggagacatgc ggtctggccg aatccgatct
      901 tcttcggatc gccggcgcag tccgcgacgc ctggcgttcc ggcggtcaat ctcggcggcg
      961 cgctgaccgt tggcgaggaa atctggcaat tggccaacga caatggtggg cttgagatcg
     1021 tcgtcaggcg gcgaaacagg cgttaacgtg aaggcttagc gaacgttccg accgcgactc
     1081 acagtccagc tatctctcgg aacgtttagt ccaccaaatc aatccagcgg ccgaagcgcg
     1141 ataagatctg atgtcgttgc aaaggtataa tgcgcgcgag caagttcgag ggcctcgtcg
     1201 cgtgcagccg gcttacgcga gacgcgcagg ttgatcgtgt tggcgtcgag gccgacgaac
     1261 tcggcgccgt aacggtcgcg ccagacgcgc agcgccgcga cgcgatcggc gggcggaggg
     1321 caggcgttcc aatttcccaa gctcatatag gctggagcgg tggtaggacg tgggtcggga
     1381 tcaacgcaat gcgcacggtc gaccttggct gtcccgtcgt gaaaaatcgc gcacgcccga
     1441 caaatccctg cgtagctttg tttaccgact caatagacat tctcctcacg gtacgcggtt
     1501 ccggcgccga accgcatttg atatccaatc gagctctcac cggcaatgta gtcgcaaccg
     1561 cttgtgacgc cgagaagtcg cgcggttcca gcgacggtgt gcctggggac ccgcgcctcg
     1621 cccggtgtca aggcgatcat tcaatcgcct ctgcgccaat acgcgtcttc gagctgcaat
     1681 agatgctgac gcatggccgc ctcggcggcg tccgaatcgc gcgcctcaat cgcttcatag
     1741 atatgcgcgt gcgcattata ggcgatccga tcttggcctt ggcgctctaa cgtgaccgtc
     1801 cgctgctgcc tcagccagga agacatcgcg tcgtggagcg cgatgaagac cggattgccg
     1861 gtgatttcag caagcacgaa atggaacgcg atgtccgtcg cgatgaagag gtcgcgatcg
     1921 ccgatcgcgg ctttattggc ggcgagcgcc gcgcggagtt tttcgacttg cgcctcgttc
     1981 gcctcgcgcg cggcctgacg ggcgaggccg cattcgaaga acatcctgac gcgctggaaa
     2041 tgatgctgac cgtcctcggt cgagagcatc tggagcacgg ccggcatcat gcccgcgacc
     2101 acgtccgagg ccgtcggcat cgaaacgcgc gccggagcgc cgtttgaaat ttcgatcaag
     2161 ccggcgcgct gcagcgtgat gagcgcttcg cggatcgccg gccggccgac gccgaaccgc
     2221 gcctgcaatt cgcgctcggt cggcaggcga tcgccaggcc gcaacgcgcc gtccagaatc
     2281 tggcgctcga tatgggcggc gacctgctcg aagagctttt gccggctgat tgcgattggc
     2341 tcttgcattc gacctatcat gccatcatgc ttgacagcca tcatacatac agccagtatg
     2401 ttaggcaagg cggccgtcag cgccgtttga tcggctaaat cgagccaatc cggaaacgtc
     2461 ggcccggcca gaatcgccgg gcaatcagag ggtcacgatg aaaatcgcga ttttgggagg
     2521 cggcggcgcc atgggcggga ttttcggcgg ctggctggcg gccgccggcg aggacgtcac
     2581 gctgatcgac gtttcgcgcg ccgcgatcga gcagatccgc gccaggggat tgcgcatcga
     2641 cgagaaggac ggttcgagcc gcgttgttcc ggtcgcggcg acgaccgcgc cggccgaaat
     2701 cgggccggtc gatctcatcg tcaatttcgt caagtgctat cacaccgatg cggcgatccg
     2761 ggcggcgcag ccaatgctcg gaccggacgc cgccgttctg acgctgcaga acggttgggg
     2821 caatgcggac aagatcgccg ccctggtcgg ccgcgagcgc gtcctggtcg gcctgaccta
     2881 tcacggcggc acgctgctcg gccccggcca tgtgaagcat ccgggcgtcg gcatgaccta
     2941 tgtcggcgag ctcgacggct cgaccagtcc tcgcttgcag cgtgtggtcg aagctttccg
     3001 gcgggcaggg ttcgagacca cgctatcgag ggcgatcctc gacgaggtct ggaaaaagct
     3061 ggcgctcaac gcctgcacgc tgccgaccgc cgcgctgctg cgattcatgg cgcatcagct
     3121 cgtcgcccat gccggcgtcg aagccgagat gcgctcgatc ctcgaagaag tcgtcgccgt
     3181 cgccaaggcg caggggattg cgctcgactt cgaagagcgc tggacggcga tttgcggcct
     3241 gctcgaaaaa gcgatcggcg gcaaggcgtc aatgcttcag gacgtcgagg cgaggcgcca
     3301 gaccgaaatc gaggtcatca acggcgcgat cgtcgcggcg ggacaaaagc atggcgtcgc
     3361 cacgccgcat aacgaggcga tggtccgcct gatcaagagc ctcgaagaag cctatctcgc
     3421 cgacgcggcc aaggagcgcg cggcgtcatg accgggcggc tcgacggcaa ggttgcgatc
     3481 gtcaccggcg ccgcgcgcgg catcggcgcg gcgatcgtgc gacgttttgt tgcggaaggc
     3541 gcccgggcgg cgttgctcga cctcgatcgc cggggagggg aggatgttgc gcgctcgctc
     3601 ggcgcggcgg cgcgtttttt cccatgcgac gtaacgcgcg ccgccgattg cgaccgcgcg
     3661 gtcgccgcgg cggccagcct tttcggggcg gcgacgatcc tc
//
LOCUS       DATGJW010000170         2623 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1379378, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000170 DATGJW010000000
VERSION     DATGJW010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2623)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2623)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2623
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1379378"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..502)
                     /locus_tag="VKU03_05125"
     CDS             complement(65..502)
                     /locus_tag="VKU03_05125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ70676.1"
                     /translation="MTGWLLDTNILSELRRPKAERRVLSFVAAQPLDFLYVSTATLAE
                     IRFGIERASDVSRRADLHDWLEHKVRPMFEQRVLAITEDVMFKWRILVEEGRKARHTF
                     SQPDLIIAATALHHGLTVVSRDVSDYKRARVPVFNPWTDPLAE"
     gene            complement(499..801)
                     /locus_tag="VKU03_05130"
     CDS             complement(499..801)
                     /locus_tag="VKU03_05130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HLJ70677.1"
                     /translation="MKSSKLAKPHRRARAQSGQWLLHDAKARFSELVRRVRSEGPQRV
                     TVHGRDEVVVITAEDFRRLKGDLTGEALIAAMQGSPQREFDLEPKRESMPVREVSL"
     gene            complement(863..1618)
                     /locus_tag="VKU03_05135"
     CDS             complement(863..1618)
                     /locus_tag="VKU03_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70678.1"
                     /translation="MKAFRDRLIGFAVVALLVALWQAVANLKLVSPVYLPGPDRAFTA
                     LVHGLSASDLGAKLAGTVEHMVEGWLLASIAGIALGALIGSSRQARAFIAPTLEFLRP
                     LPASAIIPVAIAIFGLSPAMALGVIAFGAIWPMLLATIHGFAAVEPRLYEVAGALRLS
                     RPAVIFKIALPSALPDILAGLRLGLTVALILAVVCEMIAGLNGLGNWVLLSARMFRSA
                     DLFAGVILLGAVGFAGALALNAAERRLLAWRAP"
     gene            complement(1615..2373)
                     /locus_tag="VKU03_05140"
     CDS             complement(1615..2373)
                     /locus_tag="VKU03_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLJ70679.1"
                     /translation="MKIDGRAFVLPILLVLLMEFAARAHGPSDSLAPPSAIAAAFYEA
                     LGDGSLLTATRDTLASAFAGLALGGAIGLVVGVMLGAAPAANRALEAAIELTRPIPSV
                     ALVPIALVALGFGYRLEIAIVAYACVWPLLLLTRAAVAGVEPRLVEVARALRLGPVAR
                     VVKIVAPAALPRIFVAFRLAAGVALIVAVTVEIAVNPIGLGAGIMTAGQALRPDLMLA
                     YLVWIGIVGFALNALLILAQKRFFGRAAQAAELP"
     gene            complement(2370..>2623)
                     /locus_tag="VKU03_05145"
     CDS             complement(2370..>2623)
                     /locus_tag="VKU03_05145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007773163.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70680.1"
                     /translation="ELQDELLNIVAASGATVFFVTHDLEEAIYLGDRVIGLLPHPGRV
                     GVELAIDLPRPREQVTTREAPEFLRLRRALYDFIKSAER"
BASE COUNT          508 a          828 c          909 g          378 t
ORIGIN      
        1 agcgacgagg ccggcgagaa cgccgacctg gggctcggcc ctccggcccg attgatcgct
       61 cggcttactc ggccaaggga tcggtccacg gattgaatac ggggacccgc gctcttttat
      121 aatcgctgac gtcacgagat acgaccgtca gaccgtggtg gagcgcggtt gcggcgatga
      181 tgaggtcggg ctgcgagaag gtatggcggg ccttgcggcc ttcttcgacc agaatacgcc
      241 acttgaacat aacatcttcg gtaatggcga gcacgcgctg ctcaaacatt ggccgtactt
      301 tgtgctccaa ccagtcatgg aggtcggcgc gacggcttac gtcgcttgca cgctcaattc
      361 caaatcgaat ctcggcgagc gtcgccgtgc tgacatagag aaaatcgagc ggctgagccg
      421 cgacgaagct aaggactctt cgttcggctt ttgggcggcg cagttcggaa agaatgtttg
      481 tgtccagaag ccagccggtc acaaggagac ttctcgcacc ggcatggatt cgcgcttcgg
      541 ctcgagatcg aactctcttt gcggcgagcc ttgcattgca gcgatcagcg cctcgcccgt
      601 gagatcgccc ttgaggcggc gaaagtcctc ggcggtgatg acgacgactt cgtcgcggcc
      661 gtgaactgtc acgcgctgcg gcccctcgct tcgcacccgg cggaccaact cgctgaaacg
      721 cgccttggcg tcgtgcagca gccattggcc ggattgcgct cgagccctcc gatgcggctt
      781 agccagtttg gatgatttca ttattctagt catcttgacc agattatccg gccgacgacc
      841 tgatttcaag gcccttaagg agctatggcg cgcgccaggc gagcaggcgc cgctcggcgg
      901 cgttcagcgc cagcgcgccg gcaaagccga ccgcgccgag cagaatgacg ccggcgaaga
      961 gatcggcgga gcggaacatg cgcgccgaga gcagaaccca gttgccgagc ccgttcaggc
     1021 cggcgatcat ctcgcagacc acggcgagga tcagcgcgac ggtcaggccg agcctcaggc
     1081 cggccaggat gtcgggcagc gccgagggca gggcgatctt gaaaatcacc gccggccgcg
     1141 acaggcgcaa ggcgccggcg acttcataga gccgcggctc gaccgcggcg aagccgtgga
     1201 tcgtcgccag cagcatcggc cagatcgcac cgaaggcgat gacgccaagc gccattgccg
     1261 ggctcaagcc gaaaatcgcg atggccaccg gaatgatcgc ggaggcgggc aagggccgca
     1321 ggaattccag cgtcggcgcg atgaaggcgc gcgcctggcg cgacgagccg atcagcgcgc
     1381 cgagcgcgat tccggcgatc gaggccaaaa gccagccttc gaccatatgc tcgaccgtgc
     1441 cggcgagctt ggcgccaaga tcgctcgcgc tgaggccatg aaccagcgcg gtgaaggcgc
     1501 ggtcgggacc tggaaggtag actggcgaaa ccagcttgag attggcgacg gcttgccaga
     1561 gcgcgacgag cagcgccacg acggcgaagc cgatcagacg gtcgcgaaag gctttcatgg
     1621 caattcggcg gcttgcgccg cgcggccgaa aaaccgcttc tgcgccagga tcagcaaggc
     1681 gttgagcgcg aagccgacga tgccgatcca gacaaggtaa gcgagcatca ggtcgggccg
     1741 cagcgcctgg ccggcggtca tgatgccggc gccgagcccg atcggattga ccgcgatctc
     1801 gaccgtgact gcgacgatga gggcaacgcc ggcggcgagg cggaaggcga cgaaaatgcg
     1861 cggcaaggcg gccggcgcga cgattttcac gacccgcgcg actgggccga ggcgcagagc
     1921 gcgggcgact tcgacgaggc gcggctcgac gccggcgacg gcggcgcggg tgagaaggag
     1981 cagcggccaa acgcaggcat aggcgacgat cgcgatctcc agccgatagc cgaagccgag
     2041 cgcgaccaga gcgatcggca cgagcgcaac cgacgggatg ggccgcgtca actcgatcgc
     2101 cgcttccagc gcgcgattgg cggcgggcgc agcgccgagc atgacgccga caacgaggcc
     2161 gatggcgccg ccgagcgcca agccggcgaa ggcgctcgcc agcgtatcgc gcgtcgccgt
     2221 cagcagtgag ccgtcgccga gcgcttcata aaaggccgcc gcgatcgcgc ttggcggcgc
     2281 caggctgtcg ctcggcccgt gcgctcgcgc ggcgaactcc atcagaagga cgagaaggat
     2341 cggcaggacg aaggcgcggc cgtcgatctt catcgctcgg cgctcttgat gaaatcgtaa
     2401 agagcgcgcc gcaggcgcag gaattccggc gcttcgcggg tcgtcacctg ctcgcgcggg
     2461 cgcggcaggt cgatcgcgag ctcgacgccg acgcggccgg gatgcggcag caggccgatc
     2521 acccgatcgc cgagatagat cgcctcttcg agatcgtggg tgacgaagaa cacggtcgcg
     2581 ccgctcgcag cgacgatgtt gagcaattcg tcctgcaatt cct
//
LOCUS       DATGJW010000171         4367 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_756849, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000171 DATGJW010000000
VERSION     DATGJW010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4367)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4367)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4367
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_756849"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /locus_tag="VKU03_05150"
     CDS             <1..581
                     /locus_tag="VKU03_05150"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HLJ70681.1"
                     /translation="GSADRRIFLLVVLATIPAVIVGTVARKLLGDAFGSPMTAAIFLI
                     VNGFILFAGDRIAGRSVGVLDQLNWKGALAIGVAQCFALIPGISRSGATIVAGVVAGL
                     QHKDAARFSFLMATPIILGATAHEAPALFKQGATLGGAAILSGVVAGIVAYISTTILM
                     RYFNKHEFEALNPFAYYCWAAGAVSVALIALT"
     gene            complement(681..2789)
                     /locus_tag="VKU03_05155"
     CDS             complement(681..2789)
                     /locus_tag="VKU03_05155"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLJ70682.1"
                     /translation="MNLFGLFKPSVKIRTLLLVAVPMLGLAAIGAATFLGDRAVREGD
                     QKRAATGALSLASFNLISGAAELQSLGLRVFQQPSTEVEGKFAERLKALTVSLPGGGQ
                     AAAGALAPLAADLKAVGETFAGLTVLNEEFGRTPKQGLQKDFLDVGKDIDRATRSLLV
                     GAQGAGSFGLLEAVLVMRRIASDFRLTRDHKLIDAFADEKKAFADALAQVNAPDEHKS
                     TLKLLIDGYGAAFQRWTAVQMQRAEAFDKLEAAIAGLQSEAAASNTKAAAASRQAGAD
                     AAAAMSKASIWSASVILATFLFCGLFGVLTALGISRPLGRLVGNLRDIAMGRDEIDVA
                     DLDRRDEIGELARAVKAFQDAGIERVRLTRDAAEARRRADDERAAAEMTSAEGARQQA
                     RVVQFLATGLERASGGDLVFRLTEPFSPQYEKIRADFNLAMEKLQEAMRAIAASTEEI
                     HAGAGEIRAAADELSGRAEREAASLAETAAALNQATASVQRTAEDTGRATSVIAAAKT
                     MAERSGEVARHATQAMGGIQSSAKKIGQIIGVIDEIALQTNLLALNAGVEAARAGKAG
                     AGFAVVASEVRALAQRSAEAAKEIKALVSASISQVGQGVELVGQTGAAQGEIVARVVE
                     TATIVSRIAQSAQEQAAGLRQINSAVGDLDKATQQNAAMVEESTAASHRLAGEAEELA
                     RLVARFRLDAEGAAAQSRAA"
     gene            3004..3486
                     /locus_tag="VKU03_05160"
     CDS             3004..3486
                     /locus_tag="VKU03_05160"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ70683.1"
                     /translation="MNPNRTGDALRRLINLVSHRSGSVLAVMDQAAITLPQILLLSRI
                     ERLGTASLSDLAEHSFASTAALSQMIERLVQQDWVSRAEDRVDRRRKAIQLTPKGRRL
                     LRRMEAARSSDYEIGLAPLSQELRAQLAAVAEKAAAEIESSFHGGTQTPLLRARQARR
                     "
     gene            3489..3947
                     /locus_tag="VKU03_05165"
     CDS             3489..3947
                     /locus_tag="VKU03_05165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70684.1"
                     /translation="MIESQWLLLIVCALSCYGAGQIWLVQLSSYPLWTYVGENEFRAY
                     HAAWWRGIWGVILAPATLLTAAAIAMLWLRAPGVPRWATWLGCALQAALVLGTATWWG
                     PLMARLEKPDGGLSPERYRLLMSTHWLRVAIVTAYCVLALWMFIQSAWGT"
     gene            complement(3967..>4367)
                     /locus_tag="VKU03_05170"
     CDS             complement(3967..>4367)
                     /locus_tag="VKU03_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase family protein"
                     /protein_id="HLJ70685.1"
                     /translation="AYGADVGAAPPGDLSARKLHAPFFRFPGFAETPDLRSYLAANNI
                     ATFGTDLWAADWIKMTPQKEIGVLMDALDRAGRGMILLHDDKPWTAAMLPDFLRTLKS
                     RGYRVVHVVAGEGRAETKPAAKNWKAAAPP"
BASE COUNT          666 a         1454 c         1445 g          802 t
ORIGIN      
        1 gcggatcagc ggatcgccgc atctttctgc tggtcgtgtt ggcaacgatt ccggcggtga
       61 tcgtcggaac ggttgcgcgc aagcttctcg gcgacgcgtt cggctcgccg atgaccgcgg
      121 cgattttcct gatcgtcaac ggcttcatcc tgttcgccgg cgacaggatc gccggccgat
      181 cggtcggcgt gctcgaccag ctcaattgga aaggcgcgct ggcgatcggc gtggcgcaat
      241 gcttcgcgct catcccgggc atttcgcgct ccggcgcgac gatcgtcgcc ggcgtcgtcg
      301 ccggcctgca gcataaggac gccgcgcgct tctccttcct gatggcgacg ccgatcatcc
      361 tcggcgcgac ggcgcacgag gcgccggctc tgttcaagca aggcgcgacc ctcggcggcg
      421 cggcgatcct ctccggcgtg gtcgcgggga tcgtggccta tatctcgacg accattctca
      481 tgcgctattt caacaagcac gaattcgagg cgctgaaccc gttcgcgtac tattgctggg
      541 cggcgggggc ggtgagcgtc gccctgatcg cgctgacctg agcgctcatc accgagacga
      601 tgggtgcttc tgccccgcct ccccaaagca attgcgcgcc gactctcgac ctaagcgaag
      661 ccgtcacacg ggccggcgcc ttacgccgcg cgcgattgcg cagccgcccc ttcagcgtcc
      721 agccggaagc gcgcgaccag ccgcgccaat tcctccgcct cccccgccag cctgtggctc
      781 gccgccgtcg attcctcgac catcgcggcg ttctgctgcg tcgccttgtc caaatcgccg
      841 accgcgctgt tgatctggcg aaggccggcc gcctgctcct gcgccgattg cgcgatgcgg
      901 ctgacgatcg tcgcggtctc gacgacgcgt gcgacgatct cgccctgagc cgcgccggtc
      961 tggccgacaa gctcgacgcc ctggccgacc tggcttatcg aggccgagac cagggctttg
     1021 atctctttcg ccgcctctgc cgagcgctgc gccagggcgc gcacttccga ggcgacgacg
     1081 gcgaagccgg cgcccgcctt gccggcgcgc gccgcctcga cgccggcgtt caaggcgagc
     1141 agattggtct gcaacgcgat ttcgtcgatg acgccgatga tctggccgat cttcttggcc
     1201 gaggactgga ttccgcccat cgcctgagtc gcgtggcggg cgacttcgcc ggagcgctcg
     1261 gccatcgtct tggcggctgc aatgaccgac gtcgcccggc cggtgtcttc ggcggtgcgc
     1321 tgcacgctcg cggtcgcctg gttgagggcc gcggccgtct cggcaaggct cgccgcctcg
     1381 cgctcggcgc ggccggaaag ctcgtccgcg gcggcgcgga tttcgccggc gccggcgtga
     1441 atttcctcgg tgctggcggc gatcgcccgc atcgcctctt gcagcttctc catcgccaga
     1501 ttgaagtcgg cgcgaatttt ttcatactgc ggcgagaagg gctcggtcag gcggaagacg
     1561 agatcgccgc ccgaggcgcg ctccagcccc gtcgccagga actgcaccac gcgggcctgc
     1621 tgccgggcgc cttcggcgct ggtcatttct gcggcggcgc gttcatcgtc ggcgcgccgg
     1681 cgcgcctcgg cggcgtcccg ggtcagcctg acacgctcga ttccggcgtc ctggaaggct
     1741 ttgacggccc gggcgagctc gccgatttcg tcgcggcgat cgagatcggc gacgtcaatc
     1801 tcatcccggc ccatcgcgat gtcgcgcaaa ttgccgacga ggcggccaag cggccgcgat
     1861 atgccgagcg ccgtcaagac gccgaacagg ccgcaaaaca ggaaagtcgc gagaatgacc
     1921 gaggccgacc agatcgaggc cttgctcatc gccgcggcgg catccgcgcc ggcctgacgg
     1981 ctggcggccg ccgctttcgt gttcgaggcg gcagcctcgc tttgcaggcc ggcgatcgcc
     2041 gcctccagct tgtcgaaggc ttcggcgcgc tgcatctgca ccgccgtcca gcgctggaag
     2101 gcggcgccgt agccgtcgat cagcagcttg agcgtgctct tgtgctcgtc gggcgcattg
     2161 acctgggcaa gcgcgtcggc gaaggccttc ttttcgtcgg cgaacgcgtc gatcagcttg
     2221 tggtcgcggg tcagccgaaa atcgctggcg atgcggcgca tgacgagcac cgcttcgagc
     2281 aggccgaacg agcccgcgcc ctgcgcgccg acgaggagcg agcgcgtggc gcggtcgatg
     2341 tccttgccga cgtcgaggaa atccttttgc aggccctgct tgggcgtgcg gccgaactcc
     2401 tcgttcagca cggtcaggcc ggcgaatgtc tcgccgacgg ctttcaaatc ggcggcgagc
     2461 ggcgcgagcg cgcccgcggc ggcttggccg ccgcctggca ggctcaccgt tagcgccttc
     2521 agcctttcgg cgaatttgcc ttcgacctcg gtcgatggct gctggaagac ccgcaagccg
     2581 agcgactgca actcggcggc gcccgaaatc aggttgaagc tggcgagcga cagcgcgccg
     2641 gtcgccgccc gcttctggtc gccttcgcga accgccctgt cgccgagaaa cgtcgcggca
     2701 ccgatcgcgg ccaatcccag catcggcacg gcgacgagaa gcagcgtcct gattttgacg
     2761 gagggtttga agagcccgaa caggttcatg cggcggcttc gctgaaggat ggcggcgttg
     2821 gccccgcctt gatgatggcc cgcaacaatt aaagcgaatg cgttaacagg cgcttagggc
     2881 gcggcctcat ggggcggcgc tcttgccgag ctccggcgaa cgtgacgaaa acgcgattgg
     2941 gtctacaagt tgacaaatgc ccgttaatgt ttaatgtaga ttaagtattt cgttggctct
     3001 caaatgaatc ccaaccgaac cggcgacgcg ctgcggcgtc tcatcaatct cgtttcacat
     3061 cgctccggtt cggtcctcgc cgtcatggat caggcggcga tcacgcttcc ccagattttg
     3121 ctgttgagcc gcatcgagcg attaggaacg gcatccctct ctgacctcgc agagcattct
     3181 ttcgcttcca cggcggcgct cagccaaatg atcgagcgcc tcgttcagca ggattgggtc
     3241 agccgagccg aagaccgcgt cgatcgtcgg cgaaaagcga tccaactaac gcccaaggga
     3301 cgaaggcttt tgcggcggat ggaggccgcg cgttcatcgg actacgagat cggcctcgct
     3361 ccattgagcc aggagcttcg cgcgcaattg gcggcggtcg ccgagaaagc cgcggcggaa
     3421 atcgaatcct cgtttcatgg cggaacacag acgccgctgc ttcgtgcaag acaagccagg
     3481 agatgacgat gattgaaagt caatggctac tcctcatcgt ctgcgcgctg agttgctatg
     3541 gcgccgggca aatctggctg gtgcagcttt ccagctatcc gctctggacc tatgtcggcg
     3601 agaacgagtt tcgcgcctac catgcggcgt ggtggcgcgg catctggggc gtcattctgg
     3661 cgccggcgac tcttttgacc gccgccgcca ttgcgatgct gtggctgcgc gcgccgggcg
     3721 ttccgcgttg ggcgacgtgg ctcggatgcg cgctgcaagc cgcgcttgtc cttggcacgg
     3781 caacctggtg gggaccgttg atggcgcggc tcgaaaagcc ggacggcgga ctttcgccgg
     3841 agcgttaccg tctgctgatg tcgacccatt ggcttcgcgt tgcgatcgtc acggcttatt
     3901 gcgtcctagc gctgtggatg ttcattcaaa gcgcttgggg tacgtgagcg gcaagaatgc
     3961 ggcgctctaa ggcggcgccg ccgccttcca atttttcgcc gctggcttgg tctcggcccg
     4021 accttcaccg gccacgacat ggacgacgcg atagccccgg ctcttcagcg tgcgcaggaa
     4081 atcgggcagc atggccgccg tccatggctt gtcgtcatgc agaagaatca tgccgcggcc
     4141 ggctcgatcg agcgcgtcca tgaggacgcc gatctccttt tgcggcgtca tcttgatcca
     4201 atcggcggcc cagaggtcgg tcccgaacgt cgcgatattg ttcgccgcca ggtagctgcg
     4261 cagatcgggc gtctcggcga agcccggaaa gcggaagaac ggcgcgtgga gtttgcgcgc
     4321 cgaaagatcg ccgggcggcg ccgcgccgac atccgcgcca taggcga
//
LOCUS       DATGJW010000172         4692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_862038, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000172 DATGJW010000000
VERSION     DATGJW010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_862038"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..522
                     /locus_tag="VKU03_05175"
     CDS             <1..522
                     /locus_tag="VKU03_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter associated domain-containing
                     protein"
                     /protein_id="HLJ70686.1"
                     /translation="APPSMPALDLLLKMQTTRTHMALVIDEYGGCDGLVSIEDVVEAI
                     VGDIEDEHDEADTPAVEKTADGAFIADARASLEDLSTALGDDFAKFGEAEDVDTVGGL
                     VTAKAGRVPGRGEIVGGIGDYEFEILDADPRRVKKVKIQRAAPPAKKSGARRKRAGAA
                     PPSETVAGGGEGG"
     gene            647..2311
                     /gene="lnt"
                     /locus_tag="VKU03_05180"
     CDS             647..2311
                     /gene="lnt"
                     /locus_tag="VKU03_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016410 - N-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006497 - protein lipidation [Evidence
                     IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="apolipoprotein N-acyltransferase"
                     /protein_id="HLJ70687.1"
                     /translation="MTLGVSISGDRASFIQSIGEPVILAWGWRRRLIGLAAGAAGALA
                     LAPVDFWPAMLAPLMIAVWLLDGSADSNADGATFLPSLRAAFTVGWWWGFGYFVADLW
                     WLGSAFLVDADQFAWALPLGVVALPAGLAFFPALGFALAKALWSPGPARIFALALGLT
                     VSEWLRAVVLTGFPWNELGMTLGGTPVLAQSASLVGLHGLTFLTIAIFASPATLIDET
                     SGKRRAAATVAALAAFALLAAFGAWRLSAPPAASVAGVKLRLIQPAIGQGPDFRPENK
                     DAIMRRYLELSDRATSPASTGVADVTHLIWPESAFPFLLARDPVALNQIAGLLHGGAT
                     LITGAARAERGSDDGRPRYYNSIQVIDRRGELLDHYDKVRLVPFGEYLPLRGLLEKLG
                     FSQFVRYAPGGFDPGTGDRLLRAPGLPAAWPMVCYEAIFPDELGALFGRRERAAFILN
                     LTDDIWFGRTAGPYQHFAQARLRAIEQGLPLVRDANSGISAVVDGLGRTIAELPLGGD
                     GVLDSALPMARPPTIYARYGALGPGALLLLGLIFCFALRGLPRKRL"
     gene            2450..2881
                     /locus_tag="VKU03_05185"
     CDS             2450..2881
                     /locus_tag="VKU03_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ70688.1"
                     /translation="MKKAPNPIDQQVGARLRMQRMLVGMSQEKLGDALGVTFQQIQKY
                     EKGSNRISASTLKQIARVLNVQPSFFLEDASPRGGAPGFAEPASGSYVVEFLSTAEGV
                     ELNRAFSSIRDAKIRKGVLDFVVLLARNLGGDNPPRQGGAA"
     gene            3057..4247
                     /gene="metK"
                     /locus_tag="VKU03_05190"
     CDS             3057..4247
                     /gene="metK"
                     /locus_tag="VKU03_05190"
                     /EC_number="2.5.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168726.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004478 - methionine adenosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006556 - S-adenosylmethionine biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine adenosyltransferase"
                     /protein_id="HLJ70689.1"
                     /translation="MTRKNYLFTSESVSEGHPDKVCDRISDEIVDLYFREGVKAGIDP
                     YQIRVACETMATTNRVIIAGETRGPELSQGAIEQAARDAIKDIGYEQDGFHWRNAKVE
                     VLLHKQSADIAQGVDASGNKDEGAGDQGIMFGYACRETPEFMPAPLFYAHKILHVLSE
                     ARRSGKEKKLGPDAKSQVTLRYENGAPVEATQIVVSHQHVDESLTSEEVREIVRPYVE
                     KALPAGWITGKTAWHVNPTGKFFIGGPDGDCGLTGRKIIVDTYGGAAPHGGGAFSGKD
                     PTKVDRSAAYAARYLAKNVIAADLADRCTIQLSYAIGVAKPLSIYADLHDTGRVDPAK
                     LEGALMDVMNLSQRGIREHLGLNRPIFARTAAYGHFGRKPEADGAFSWEKTDIAGDLK
                     RLIS"
     gene            4199..>4692
                     /locus_tag="VKU03_05195"
     CDS             4199..>4692
                     /locus_tag="VKU03_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70690.1"
                     /translation="MGEDRHCRRSEAPHLLIATPEDETAPASAPDSGKRRALYGRTHG
                     KALRSHQAALIKDLLPRLDVGVAPRLDCAALFPFSPREIWLEIGFGGGEHLAAQAAAH
                     RDVGFLGAEPFVNGLAKALAAIEREKLDNIRLRAGDAGALIAALPDAVLSRIFVLYPD
                     PWPKR"
BASE COUNT          729 a         1601 c         1548 g          814 t
ORIGIN      
        1 gcgccgccgt cgatgccggc gctcgatctt ttgctgaaga tgcagacgac gcgcacgcat
       61 atggccctgg tcatcgatga atatggcggc tgcgacgggc tggtttcgat cgaggacgtg
      121 gtcgaggcga ttgtcggcga catcgaggac gagcacgacg aggccgacac gccggcggtc
      181 gagaagaccg ccgacggcgc ctttatcgcg gacgcccgcg ccagcctcga agacctctcc
      241 acggcgcttg gcgacgattt cgccaaattc ggcgaggccg aggacgtcga cacggtcggc
      301 ggcctcgtca ccgcgaaagc cggccgcgtt cccggccgcg gcgagatcgt cggcggaatc
      361 ggcgattacg agttcgaaat cctggacgcc gatccgcgcc gggtgaagaa ggtcaagatc
      421 cagcgcgcgg cgccgccggc gaagaaatcc ggcgcccggc gcaagcgcgc cggcgctgcg
      481 ccgccctcgg aaacggtcgc gggcgggggg gaaggaggct gaccgcccgc gccgcctctg
      541 gaacgggctc agcctggatt ccttgttgcg cgggaatgcc gcgtttcagt ctcgtcgcgt
      601 catgctttaa ggtgaaaacg cgtgattcgc ggcaagggac ggcggcttga ccctgggcgt
      661 ctcaatctct ggagaccgcg cctccttcat ccagtcgatc ggcgagccgg tgattctcgc
      721 ctggggctgg cgccgtcgcc tgatcggctt ggcggcgggc gccgcaggcg cgctggctct
      781 ggcgcccgtc gatttctggc cggcgatgct ggcgccgctg atgatcgcgg tctggctgct
      841 cgacggctcg gccgattcga acgccgacgg ggcgacgttc ctgccgtcgc tgcgcgccgc
      901 cttcacggtc ggctggtggt ggggcttcgg ctatttcgtc gccgacctct ggtggctcgg
      961 ctcggccttc ctcgtcgacg ccgaccaatt cgcctgggcg ctgccgctcg gcgtcgtcgc
     1021 gctgccggcg ggactcgcct tctttcccgc cctcggcttc gccttggcga aggccctgtg
     1081 gtcgccgggg ccggcccgca tcttcgccct cgccctcggc ctgaccgtca gcgaatggct
     1141 gcgcgcggtg gtcctgaccg gctttccctg gaacgagctc ggcatgacgc tcggcggaac
     1201 gccggtcctg gcgcaaagcg cctcgctcgt cggcttgcac ggcctgactt tcctgacaat
     1261 cgcgattttc gcgtcgccgg cgacgctcat cgacgaaacg agcggcaaaa ggcgcgcggc
     1321 ggcgaccgtc gcggcgcttg ccgctttcgc gctcctcgcc gcgttcggcg cctggcgcct
     1381 ctcggcgccg ccggcggcga gcgtcgccgg cgtcaagctt cgcctcatcc agccggcgat
     1441 cggccaaggt ccagattttc ggccggagaa caaagacgcg atcatgcgcc ggtatctcga
     1501 actctccgac cgcgccacct cgccggcttc gacgggcgtc gccgacgtga cccatctgat
     1561 ctggccggaa tcggccttcc cgttccttct ggcgcgcgat ccggtcgcgc tcaaccagat
     1621 cgccggcctg ctccatggcg gcgcgaccct gatcaccggc gcggcccgcg ccgaacgcgg
     1681 ctccgacgac gggcggccgc gctactacaa ttccatccag gtcatcgatc gccgcggcga
     1741 gttgctcgat cattacgaca aggtccgcct ggtgccgttc ggcgagtacc tgccgctgcg
     1801 cggcttgctg gagaagcttg gtttcagtca gttcgtgcgt tatgcgccgg gcggcttcga
     1861 tccgggaacg ggcgaccggt tgttgcgcgc gccgggcctg cccgccgcct ggccgatggt
     1921 ctgctacgag gcgatcttcc ccgatgagct tggcgcgctg ttcgggcggc gcgagcgcgc
     1981 cgctttcatc ctcaatctga ccgacgacat ctggttcggc cgtacggcag ggccttatca
     2041 gcatttcgcg caagcgcggt tgcgcgccat cgagcagggt ctgccgctgg tccgtgacgc
     2101 caattccggc atttccgcgg tggtcgacgg gctcggccgg acgatcgccg aactgccgct
     2161 cggcggcgac ggcgtgctcg attcggcgct gcccatggcc cggccgccga cgatctatgc
     2221 gcgctacggc gcgcttggcc cgggggcgct cctcctcctc ggcctcatct tttgtttcgc
     2281 cttgcgcggt ctacctcgga agcgcctgta gacggcggcc ggcaaagact tgcggcggcc
     2341 cgcccattac acgtaaaacg agccttgccg acatttcagg agctgccccg tcggcgcgcg
     2401 gcttagcggc tgtgaccttt agaggaaacg gctcgaggga ttcgccggca tgaaaaaagc
     2461 tcccaacccc atcgatcagc aggtcggcgc gcgcttgcgc atgcagcgca tgctggtcgg
     2521 aatgagccag gagaagctcg gcgacgcgct gggggtcacc tttcagcaaa tccagaaata
     2581 cgaaaagggc tccaatcgga tcagcgccag cacgctgaaa cagatcgctc gcgtgctcaa
     2641 cgtgcagccc tccttctttc tcgaagacgc ttcgccacgc ggcggcgcgc ccggcttcgc
     2701 cgagccggcc agcggctcct atgtggtcga gttcctgtcg accgccgaag gggtcgagct
     2761 gaaccgcgct ttttccagca tccgcgacgc caaaatccgc aaaggcgtgc tcgacttcgt
     2821 cgtcctcctg gcgcgcaacc tcggcggtga taatccgccc cgacaagggg gcgccgcgta
     2881 gttggattcg ttcgctatat cgaacgcttt tgcgagaatc gcttgacctg acgaacggcg
     2941 gctgtcatta gggcggcctg ccggcgccgg aacgcgtgaa ggaggcttgt tggggcaagc
     3001 tgaggcggcc cgtcgcggca atcgagacat cagccccgcg cacggagact tgaccggtga
     3061 cccgcaaaaa ttacttgttc acgagcgaat ccgtctccga gggccatccc gacaaggtct
     3121 gcgatcggat ttccgacgag atcgtcgacc tctatttccg cgaaggcgtc aaagccggaa
     3181 tcgatcctta tcagatccgc gtcgcctgcg agacgatggc cacgaccaac cgggtgatca
     3241 tcgccggcga gacgcgcggc cccgaattgt cgcagggggc catcgagcaa gcggcgcgcg
     3301 acgcgatcaa ggatatcggc tacgagcagg acggctttca ctggcgcaac gcgaaagtcg
     3361 aagtcctgct gcataagcaa tcggcggata tcgcccaggg cgtcgacgcc tccggcaaca
     3421 aggatgaagg ggcgggcgac cagggcatca tgttcggcta tgcctgccgc gagacgccgg
     3481 agttcatgcc ggcgccgctc ttctacgcgc acaagattct ccacgtcctc tccgaggcgc
     3541 gccgctcggg caaggaaaag aagctcggac cggacgccaa gagccaggtg accctgcgtt
     3601 acgagaacgg cgcgccagtc gaggcgacgc agatcgtcgt ctcccaccag catgtcgacg
     3661 aatcgctgac ctcggaagag gtccgcgaga tcgtccggcc ttatgtcgag aaggcgctgc
     3721 cggccggctg gatcaccggc aagacggctt ggcacgtcaa tccgaccggc aagttcttca
     3781 tcggcggccc ggatggcgat tgcgggctta ccggccgcaa gatcatcgtc gacacctatg
     3841 gcggcgcggc gccgcatggc ggcggcgcgt tttccggcaa ggacccgacc aaggtcgacc
     3901 gttcggcggc ctatgcggcg cgttatctcg ccaagaacgt catcgccgcc gatctcgccg
     3961 atcgctgcac gatccaattg tcctatgcga taggcgtcgc caagccgctc tcgatctacg
     4021 ccgacctgca cgacaccggc cgcgtcgatc cggccaagct cgaaggcgcg ctgatggatg
     4081 tgatgaactt gtcgcagcgc ggcatccgcg agcatctcgg cctgaaccgg ccgattttcg
     4141 cgcgcacggc ggcttacggc catttcggcc gcaagccgga ggccgatggc gctttctctt
     4201 gggagaagac cgacattgcc ggcgatctga agcgcctcat ctcctgatcg ccacacctga
     4261 agacgaaacc gcgcccgctt ccgccccgga ttccggcaag cggcgcgcgc tctatggccg
     4321 gacccatggc aaggccctgc gcagccatca ggcggcgctg atcaaggacc tgttgccgcg
     4381 gctcgacgtc ggcgtggcgc cgcggctcga ttgcgcggct ttgtttccgt tctccccacg
     4441 cgaaatctgg ctcgaaatcg gctttggcgg cggcgagcat ctggccgccc aggccgcggc
     4501 gcatcgcgat gtcggctttc tcggcgccga gcctttcgtc aacggtctcg ccaaggcgct
     4561 ggcggcgatc gagcgggaaa agctcgacaa tatccggctg cgcgccggcg acgccggcgc
     4621 gctgatcgcg gcgttgcccg acgccgtcct gtcgcgaatc ttcgtgctct atcccgatcc
     4681 ctggccgaaa cg
//
LOCUS       DATGJW010000173         7319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_757458, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000173 DATGJW010000000
VERSION     DATGJW010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7319
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_757458"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            582..>7319
                     /locus_tag="VKU03_05200"
     CDS             582..>7319
                     /locus_tag="VKU03_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012901.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ70691.1"
                     /translation="MGHLRYSLPIDRTQIAIIGRACRLPGAPNSSALWELMDSGRCAV
                     SKIPPDRWPQSLFFHSRPKERGRSYTFAAGVLDDIWGFDPSVFRISPREAEQIDPQQR
                     LLLELAFEACEDAGFAPSRLAGTRTGVYIGASALDYSTIGLHDPALGDAYFATGNTLS
                     IISNRLSYIFDLHGPSMTIDTACSSSLVALHEACQALAHGDIEAALVGGVSILASPFG
                     FITFSQATMLSPTGLCQAFSAEADGYVRAEGGVVLALKRLDRAVKDGDRIHAVICGAG
                     VNSDGRTNGVSLPSLAHQSELLRSVYDRAGVAPDSVVYVEAHGTGTQVGDPVEASALG
                     AVLGAARDQPLPIGSIKTNIGHTEPASGLAGLMKAMLALEHDRAPKSLHFKNPNPNID
                     FSELNLKVASEPILLPRTGAPRFVGVSSFGFGGTNAHVVISDPPTILSEGKASPRYLI
                     LSAQTEPALRALAGAYSERLGQAQNEEESLRVIAATAYRRERMPQRLALLADDPAELS
                     AALSRFSETGETDRKAARGAAMEGDGAIVFVFSGNGSQWPGMGQAAYRANKDFREAFG
                     EIDAIFQPLAGWSLKEQMFSPQLSDALPRASIAQPLLFAIQAASVRALAKLGVRPALV
                     MGHSVGEVAAAEAAGILALKDAIKVIYHRSRHQELTENAGGMAVVLGPRDAAEALIAE
                     FPTLSVAAYNSHRCVVAAGPYAALDALGKAAPKRKMRARQLDLAYPFHTALMDPVRRP
                     LIRDLADLAPSAGRIPFLSTISPGIMAGQSANALYWWRNVRDKVVFHEGVERAIELGK
                     RIFLEIGPNGPLKGHVRDTLEQRDVAGFVDMALDDRSNELEGDPFERTVTRLLAASAE
                     VDPEATFGPDPGAGADLPAYPWRRVAYRYGETTEATGKFSARPRHPLVGGRDNNDSLE
                     WRTILDPEIEPALADHRVDGQTILPGAAFVEMALAVARDWKGDDGVSLVGFEILQPLV
                     FAANASREILSRISASTATLEILSRPRFSKAAYALHARGGIVQKPGPALAGGTAKPVS
                     QDIDNSALYERARRCGLDFGPAYRRLARAERTAVDAIDLELTLDEGDRRYGLDPARLD
                     SCFHGLILLFDDVDSRINAYLPVRFDEVRLVKPKAQLAGAHIRIRRRDARVIVADFDL
                     FDGQGERVALLRGARYQAMRGRADVSLSQVGLIKTWIPAPAPLWGASEAPRNGFGAIE
                     IGESAPDAALPPDGLLVEGWASAASYELAQALSVDGLIDLNELVVTGRLPAARRNWAQ
                     SVFGALQRSGLVERAGGAYRLGDQRLPSAEAVFATLAAQHPHRAAELLLAAHIGATLG
                     AYGRGGGELTGAPESAREAFESRSAFALAAAKALNARLRTMAAPNKPALRILQIGHGA
                     ATSETLRFAAERGASVTILDLDARRLERARISHSRAPETAFFSDLDALPNDAFDLVVS
                     VGGLSAFCAKRGALARLFEKCAANAAFAAIEPAPSLFQNLVFGLDDDWFDEEGLRLRS
                     SEEWSALFARSGAAGTKAALVATEADPAVLILAQAPGKAAKAGFSGEVVVLREKCDVD
                     PFADALKEALAAGGAVCRFVPPKDIPSVKAEKTAKLLWLAPEAKGDGVARVAAHCLAL
                     KDLALRPSAGKLQIFTAVRRVDGPAAEAVFSFVRTLANEAPAIDFRRIAMAGETAEIA
                     KKLAAFVLSDTDETDIAIHNDSVKALRYDAPPIPEEASGAGEDLRTYRLEKSVDGGLD
                     RLHWRPALRRAPKAGEVEVEVLATGLNFRDVMWSLSVLPDEMLEDGFAGPTLGLEFSG
                     RVTRLGEGVANLRVGDDVVGFCGGAFSAHLTVDAEHVAPLPATISSEAAATIPVAFLT
                     AYYGLISCADLKRGEWVLIHGGAGGVGLAALQIAQWRGARAIVTAGSPEKRDLARALG
                     AEYAFDSRSGAFADEVMAATEGRGVAVVLNSLAGEAMERSIGLLQPFGRFVELGKRDY
                     LANTPIGLRPFRRNLSYFGVDLDQLLKARPDLSRRLFADTLALFASGDLSPLPYSVFA
                     HSEIVDAMRLMQQSGHIGKILVRPPTESPAAGAAKRPFVVNPNGTHLITGGLGGFGLA
                     AALWLIERGARHLVLVGRSGAANEEAREAVARMRSLGAEVLIEALDIADPKSSEAMFK
                     KIAVSMPPLVGVMHAAMVLDDAIVANMDEARLLKVLWPKIAGAEILDQITRDMALDYF
                     VLFSSATTIIGNPGQGGYVAANGFLEGLARRRRQAGRPALAVAWGAIADVGVLARK"
BASE COUNT         1153 a         2415 c         2413 g         1338 t
ORIGIN      
        1 tgtcatcgga ttcggccgcc gcgagcgaca cgatatgggc gaagaaattt gcggcggacg
       61 gccgggagcg ccggaaaggg cgcaacgccg ctccaacatg cgccgcaatg ccacgaaccc
      121 ccaaatcgag caatttctaa acaaccccgc tgaacttgcg ccacccctac atcgcgcaag
      181 ccgaaaaggc tacggctttg ggcgcggtct gccccctcgc cggaataggc tatccgattc
      241 ccaaaccgtt gcgcgctggc atggcgacga gaactctttc ggggaaacgt cgcttgttgg
      301 agcgcagagt gacggacgaa tgtgtttggt gaatcttgga ggagcttgcc cacatttgag
      361 cagcccatgc ccattgaaaa ggagggctaa tgtccgacgt cgcgaatcgc gaagctcatc
      421 acgaaattgt gatcgctcgg cgcttttgat gctcattgta ctcgcgcagg atttcaaatc
      481 ttggtttcat tgtgaaataa tcgaacaaaa gtcgcattcg gaggcgaaca aggccacggc
      541 ggtttggtag tcttgtttca atccataaac aaaaaatcgg aatggggcat ttgcggtaca
      601 gcttaccgat cgaccggact caaattgcga ttatcggtcg ggcctgccga ctgcctgggg
      661 cgccaaacag ttccgcattg tgggagctga tggattccgg gcggtgcgcg gtctcgaaaa
      721 tcccgccgga tcgctggccg cagagcttat ttttccattc gcgcccgaaa gagcgcggcc
      781 ggagctacac gttcgccgcc ggcgtgctcg acgacatctg gggattcgat ccgagcgtct
      841 ttcgcatttc gccgcgcgag gccgagcaga tcgatccgca gcagcgcctg cttctggagc
      901 tggctttcga agcctgcgag gacgccgggt tcgcgccgtc gcgattggcc gggacgcgaa
      961 ccggcgtcta tatcggcgcc tcggcgctcg attattcgac gatcgggctg cacgatccgg
     1021 cccttggcga cgcctatttc gcgaccggca acacgctgtc gatcatttcg aatcggctgt
     1081 cctacatttt cgacctgcac gggccgagca tgacgatcga cacggcctgc tcctcgtcgc
     1141 tggtcgcgct gcacgaggcc tgccaggccc tggcgcatgg cgatatcgaa gcggccttgg
     1201 tcggcggcgt cagcattctg gcttcgccct tcggcttcat cactttctcg caggcgacga
     1261 tgctctcgcc gaccggcctg tgccaggcgt tttccgccga ggccgacggc tatgtccgcg
     1321 ccgaaggcgg ggtggttctg gccttgaaac ggctcgatcg cgccgtcaag gacggcgacc
     1381 gcatccacgc cgtgatttgc ggcgccggcg tcaattcgga cggccgcacc aacggcgtgt
     1441 cgctcccttc gctcgcccat caatcggagc tcttgcgctc ggtctatgac cgggcgggcg
     1501 tcgcgccgga ttcggtcgtc tatgtcgagg cgcatggcac cgggactcag gtcggcgatc
     1561 cggtcgaagc ctcggcgctc ggcgcggttt tgggcgcggc gcgcgaccag ccgctgccga
     1621 tcgggtcgat caagaccaat atcggccata ccgagccggc ttccggtctc gccggcctga
     1681 tgaaggcgat gctggcgctc gagcacgatc gcgcgccgaa atccctccat ttcaaaaatc
     1741 ccaacccgaa catcgatttt tccgagctca atctcaaagt cgcgagcgag ccgattctct
     1801 tgccgcgaac cggcgcgccg cgcttcgtcg gcgtcagctc gttcggcttc ggcggcacca
     1861 acgcccatgt cgtcatttcc gatccgccga ccattctgtc ggaaggaaaa gcctcgccgc
     1921 gctatctcat cctgtcggcg cagaccgagc cggctctgcg ggctctggcc ggcgcttatt
     1981 ccgagcgtct cggtcaagcg cagaacgagg aggagagcct tcgcgtcatc gccgcgaccg
     2041 cctatcgccg cgagcgcatg ccgcaacgtt tggcgctgct cgccgacgac cccgctgagc
     2101 tcagcgccgc cctgtcgcgt ttttcagaga ccggcgagac cgaccgcaag gcggcgcgcg
     2161 gcgccgcgat ggagggcgac ggcgcgatcg tcttcgtttt ttccggcaac ggctcgcaat
     2221 ggccgggcat gggccaggcg gcctatcgcg ccaacaagga tttccgcgag gctttcggcg
     2281 agattgacgc catcttccag cccttggcgg gctggtcgct gaaagagcag atgttttcgc
     2341 cccaactgag cgacgccctg ccgcgcgcca gcattgctca gccgttattg ttcgccattc
     2401 aggcggcgag cgtgcgcgcc ctcgccaagc ttggcgtgcg tcccgctttg gtgatgggcc
     2461 acagcgtcgg cgaagtcgcc gcggctgaag cggccggcat tctcgcgctc aaagacgcga
     2521 tcaaggtcat ttatcatcgc agccgccacc aggagctcac cgaaaacgcc ggcggcatgg
     2581 cggtggtttt ggggccgcgc gacgccgccg aagcgttgat cgccgaattc ccgacgctgt
     2641 cggtcgcggc gtacaattcc caccgctgcg tcgtcgcggc cggcccctac gcggcgctcg
     2701 atgcgctcgg caaggcggcg ccgaaacgca aaatgcgcgc gcgccagctc gatctcgcct
     2761 atccgttcca caccgccctg atggatccgg tgcgccggcc gctgatccga gatttggccg
     2821 acctcgctcc gtccgccggg cgcatcccgt tcctgtcgac gatttcgccg ggaatcatgg
     2881 cgggccagtc ggccaacgcg ctctattggt ggcgcaatgt tcgcgataaa gtggtctttc
     2941 acgagggcgt cgagcgcgcc atcgagctcg gtaagcggat attcctcgaa atcggtccga
     3001 acgggccgct caagggtcat gtccgcgaca cgctcgaaca gcgcgacgtc gccggcttcg
     3061 tcgatatggc gctggacgac cgctccaacg agctcgaagg cgatcctttc gagcggacgg
     3121 tcacaaggct gttggcggcg agcgccgagg tcgatcccga ggcgacattc ggacccgatc
     3181 ccggcgccgg cgccgatctg cccgcctatc cctggcggcg ggtcgcctat cgctacggcg
     3241 agaccacgga ggcgacgggc aaattcagcg cgcggccgcg ccatccgctg gtcggcggcc
     3301 gcgacaataa cgactcgctc gaatggcgga ccattctcga tccggagatc gagcccgctt
     3361 tggccgatca tcgcgtcgac gggcagacca tcttgcccgg cgcggcgttc gtcgaaatgg
     3421 cgctggcggt ggcccgcgat tggaagggcg acgacggcgt ctcgcttgtc gggtttgaga
     3481 ttctgcagcc gctggttttc gccgccaatg cgtcgcgcga aatcttaagc cggatttccg
     3541 cgtcgacggc gacgctcgaa attttgagcc ggccgcgttt cagcaaggcg gcttacgcgc
     3601 tccatgcccg cggcggcatc gtccagaagc cgggcccggc gctcgccggg gggacggcga
     3661 aaccggtctc gcaggacatc gacaattcgg cgctttacga acgggcgcgg cgttgcggcc
     3721 tcgatttcgg cccggcttat cgccggctgg cgcgggccga gcgaaccgcc gtcgacgcga
     3781 tcgatctcga actgacgctt gacgaaggcg atcgccgata cgggctcgat ccggcgcggc
     3841 tcgattcgtg tttccacggc ctcatcctgc tcttcgacga tgtcgattcg cggatcaacg
     3901 cctatttgcc ggttcgattc gacgaggtgc ggctggtcaa gccaaaggct caattggcgg
     3961 gcgcccatat tcgcattcgc cgccgcgacg ctcgagtcat tgtcgccgat ttcgatctgt
     4021 tcgacgggca aggcgagcgc gtcgcgctgc ttcgcggcgc gcgctatcag gcgatgcgcg
     4081 gccgcgccga tgtcagcctc tcgcaagtcg gcctgatcaa gacctggatt ccggcgccgg
     4141 ccccgctctg gggcgcaagc gaggcgccgc gcaatggctt tggcgcgatc gagatcggcg
     4201 agagcgcgcc ggacgcggcg ttgccgccgg acggcctgct cgtcgagggc tgggcgagcg
     4261 ccgcctctta cgagcttgct caggcgctga gcgtcgacgg cctcattgat ctcaacgaac
     4321 tcgtcgtgac cggccgcttg cccgccgcgc gccggaattg ggcgcaatcc gtctttggcg
     4381 ccttgcagag gagcggcctc gtcgagcgcg ccggcggcgc ttatcgcctc ggcgatcaac
     4441 gcttgccttc ggccgaggcg gtcttcgcga cgctcgcggc gcaacatccg catcgcgccg
     4501 cggagctctt gctcgccgcc catatcgggg cgacgcttgg cgcctatggg cgcggcggcg
     4561 gcgaactgac cggcgcgccg gaatcggcgc gcgaagcctt cgaatcgcgc tcggctttcg
     4621 ccttggcggc cgccaaggcg ctcaacgccc ggcttcgaac gatggccgcg ccaaacaagc
     4681 cggcgctccg catcctgcaa attggccacg gcgcggcgac gtctgagacg ctgcgtttcg
     4741 ccgccgaacg cggcgcgtcc gtcacgatcc tcgatctcga cgcgcggcgg ttggagcggg
     4801 cgcggatcag ccatagccga gcgccggaga cggcgttctt tagcgatctg gacgctctgc
     4861 cgaacgatgc tttcgatctc gtcgtgagcg tcggcggact ctcggcgttt tgcgccaagc
     4921 gcggtgctct cgcccgcctg ttcgagaaat gcgccgccaa cgccgccttc gccgccatcg
     4981 agcctgcgcc gtctttgttt caaaacctgg tttttgggct tgatgatgat tggttcgacg
     5041 aggagggcct gcgcctgcgc tcgagcgagg aatggagcgc gctgttcgcg cgctccggcg
     5101 cagccgggac gaaggcggcc ctcgtcgcca ccgaggccga tccggccgtt ctcatcctgg
     5161 cgcaagcgcc cggcaaggcg gcgaaagcgg gtttttccgg cgaggtggtc gtgttgcggg
     5221 aaaagtgcga cgtcgatcct ttcgccgacg ctttgaagga ggcgctggcg gccggcggcg
     5281 cggtttgccg gttcgttccg ccaaaggaca ttccaagcgt caaggcggag aagaccgcga
     5341 agctcttatg gttggctccc gaggccaagg gcgacggcgt cgcccgggtc gccgcccatt
     5401 gcctggcgct taaggatctc gccttgcggc cgagcgccgg caagctgcag attttcacgg
     5461 cggtgcgccg ggtggacggt ccggccgccg aggcggtgtt cagcttcgtg cgcacgctcg
     5521 ccaacgaagc gccggcgatt gattttcgcc gaatcgcgat ggccggcgag acggccgaaa
     5581 tcgccaagaa gctggcggcg ttcgtcctct ccgacacgga cgagacggat atcgccattc
     5641 acaatgacag cgtgaaggcg cttcgctacg acgcgccgcc gatccctgag gaggcgtccg
     5701 gcgccggaga ggatttgcgg acctatcggc tcgaaaagag cgtcgacggc ggccttgatc
     5761 gcctgcattg gcggcccgct ttacgcaggg cgccgaaagc gggcgaggtc gaggtcgaag
     5821 tgctcgccac cggcctcaat ttccgcgacg tcatgtggtc gctctcggtc ctgccggacg
     5881 agatgctcga agacgggttc gccggcccga ctctcggcct cgaattttcc ggccgagtga
     5941 cccgactcgg cgagggcgtc gccaatctgc gcgtcggcga tgacgtggtc ggcttttgcg
     6001 gcggcgcgtt cagcgcccat ctgaccgtcg acgccgagca tgtcgcgccg ctgccggcga
     6061 ccatttcttc agaggcggcg gcgacgatcc cggtcgcctt cctgaccgcc tattacggcc
     6121 tcatctcctg cgccgatctt aagcgcggcg aatgggtgct gatccatggc ggggccggcg
     6181 gcgtcggcct ggcggccctg caaatcgcgc aatggcgcgg cgcgcgggcc atcgtcaccg
     6241 ccggctcgcc ggagaagcgc gatctcgccc gcgcgctggg cgccgaatac gcttttgatt
     6301 cgcgatccgg cgctttcgcc gatgaagtca tggcggcgac ggaagggcgc ggcgtcgcgg
     6361 tggtgttgaa ctctctcgcc ggcgaggcga tggagcgcag catcgggctg ttgcagccgt
     6421 tcggccgttt cgtcgaactc ggcaagcgcg actacctcgc caacacgccg atcggcctca
     6481 ggccgttccg gcgcaactta agctatttcg gcgtcgatct cgaccaattg ctgaaggcgc
     6541 ggcccgatct gtcgcgccgg ctcttcgccg atacgctcgc gctcttcgcg tccggcgact
     6601 tgtcgccgct gccctatagc gtcttcgccc atagcgaaat cgtcgacgcg atgcggctga
     6661 tgcagcaatc cggccatatc ggcaaaatcc tggtgcggcc gccgacggag agcccggcgg
     6721 cgggcgccgc aaaacgcccg ttcgtcgtca atccgaacgg cacgcatttg atcaccggcg
     6781 gtctgggcgg ctttggcttg gccgccgctt tgtggctgat cgagcgcggc gcgcgtcatc
     6841 tggtcctggt cggccgttcc ggcgcggcca atgaggaggc gcgcgaggcg gtggcgcgga
     6901 tgcgcagcct cggcgccgag gtgctgatcg aagcgctcga catcgccgac ccaaaatcat
     6961 ccgaagcgat gttcaaaaaa atcgccgttt cgatgccgcc gctggtcggc gtcatgcatg
     7021 cggcgatggt tctcgacgac gcgatcgtcg cgaatatgga cgaggcgcgc ctgctgaaag
     7081 tgctctggcc gaaaatcgcc ggcgcggaga ttctcgatca aataacccgc gacatggcgc
     7141 tcgattattt cgtcctgttc tcttccgcga cgacgatcat cggcaatcct ggccagggcg
     7201 gctatgtcgc cgccaatggc ttcctcgaag gtttggcgcg ccgtcgccgc caagccggtc
     7261 ggccggcgct cgccgtcgcc tggggagcga tcgccgatgt cggcgtcctg gcgcgcaag
//
LOCUS       DATGJW010000174        10701 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1380376, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000174 DATGJW010000000
VERSION     DATGJW010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10701)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10701)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10701
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1380376"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..640)
                     /locus_tag="VKU03_05205"
     CDS             complement(257..640)
                     /locus_tag="VKU03_05205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70692.1"
                     /translation="MPTKAARKASTPEAAEPLADLLRRVAAELQNAAHAVDAIPVADG
                     DDSTSAPPRPLTQALQELDHTYQKLTCLADFLNAIAQSAPPQWALDPNPAAEGVTLAA
                     LAERLRSPAAPAARDDEANGEFELF"
     gene            complement(653..1195)
                     /locus_tag="VKU03_05210"
     CDS             complement(653..1195)
                     /locus_tag="VKU03_05210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234838.1"
                     /note="catalyzes the conversion of glutamine residues to
                     glutamate on methyl-accepting chemotaxis receptors;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheD"
                     /protein_id="HLJ70693.1"
                     /translation="MLRQSGKKYIRVIQGECHVSDDPDVVLTTILGSCVAACIRDPKA
                     GVGGMNHFLLPGDASRLSPSSEAERYGAYLMEVLVNGLLRRGASRQRLEAKLFGGASV
                     VKSLSPIGRMNAEFARKFLATEGIKIVSESLGDERGRRVEYRPVSGQARQLFVEAPAE
                     ILEPPPPPRAASAAGAIELF"
     gene            complement(1195..1581)
                     /locus_tag="VKU03_05215"
     CDS             complement(1195..1581)
                     /locus_tag="VKU03_05215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ70694.1"
                     /translation="MSIAQQLKVLVVDDTTTSRMLVIDALQEIGFRQIAYASNGQQAL
                     KHMMTTPAHLVISDYEMPGLDGVGLLQGIRAYQPTRHVPFVMVTGRGDKSLIERARQF
                     GLNNFVTKPFTVASLRAALQAIIKGL"
     gene            complement(1583..2629)
                     /locus_tag="VKU03_05220"
     CDS             complement(1583..2629)
                     /locus_tag="VKU03_05220"
                     /EC_number="3.1.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003522763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0008984 - protein-glutamate methylesterase
                     activity [Evidence IEA];
                     GO_function: GO:0050568 - protein-glutamine glutaminase
                     activity [Evidence IEA];
                     GO_process: GO:0006482 - protein demethylation [Evidence
                     IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0018277 - protein deamination [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis response regulator protein-glutamate
                     methylesterase"
                     /protein_id="HLJ70695.1"
                     /translation="MSAVRVLVVDDSRTMRGLISAELLRDPSIQVVGEAANAQEAREA
                     MKALNPDVVTLDIEMPEMDGLSFLEKIMRLRPTPVIMVSTLTSRGAECTVRAFELGAV
                     DCVAKPSPGDEHSLAGLAAKVKAAASARLVGLSQRLAPANGSTRGQAAAYEPDGRVVA
                     IGSSTGGVEALLNVLSKFPANCPPTVITQHIPGLFTKSLAERLNRSCAPSVAEAYEGA
                     PLKIGQVYLAPGSVAHLEIGGPPGDPRCRLKETSPVNGHRPSVDVMFFAIAKAMGRKA
                     VGVILTGMGQDGAHGLLAMREAGASTLGQDEATSVVYGMPKAAFELGAVEKQVSLARM
                     GDQLIAVTNRKGKG"
     gene            complement(2626..3525)
                     /locus_tag="VKU03_05225"
     CDS             complement(2626..3525)
                     /locus_tag="VKU03_05225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008983 - protein-glutamate
                     O-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-glutamate O-methyltransferase CheR"
                     /protein_id="HLJ70696.1"
                     /translation="MNFSALIRDAEDKDADFAQSEFEMSSRDFKHIAATLHAESGIYI
                     PESKTALVYSRLAKRLRALGLASFRDYCSLIASDDGVEERQRMIAALTTNVTAFFREP
                     HHFEHLKAHMGPALIAAAQRGERVRLWSSACSSGQEPYSIALALLSLAPNIANYDVKI
                     LATDIDPNVVASGERGLYSASALAGVPTNLRERWFRPARGENGEMWRVAEEARRLVAF
                     RQLNLIGPWPMKNPFQTIFCRNVVIYFDQPTQSKLWSRFASALAPGGVLYIGHSERLS
                     GPAAAAFRSEGVTTYRLCEGARR"
     gene            complement(3522..3971)
                     /locus_tag="VKU03_05230"
     CDS             complement(3522..3971)
                     /locus_tag="VKU03_05230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheW"
                     /protein_id="HLJ70697.1"
                     /translation="MEAAEPRKVVAFRIGNQEFCVDIMSVREIRCWTPATPLPRSPDF
                     VRGVINLRGFVLPIIDLAARFGFPPAEPTAQNVIIVTQIGQRTVGLLVDAVSDILTVT
                     DDLVQPTPDIASDLAATFVRGVFVIEGRMISFVALDGVVPAAEREAA"
     gene            complement(3999..6200)
                     /locus_tag="VKU03_05235"
     CDS             complement(3999..6200)
                     /locus_tag="VKU03_05235"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chemotaxis protein CheA"
                     /protein_id="HLJ70698.1"
                     /translation="MASDAIADIKTTFFQECEEQLAELEAGLLALEGGEADLETVNAI
                     FRAVHSIKGGAGAFHLDDLVRFAHAYETTLDHLRSGQISATPAGTKVMLRAADMLADL
                     VRAARDGGAADIARGDGIIEELVALSGAAEESAANDDGDGMDGLDFTPVQVSFDTNSI
                     DEAAAAAGDAPSSGRYVVEFMPKPALYAKANEAGLLLRELARLGEAVVSCDIGETPLL
                     PDLDPEGAYLRWTVTLPGDVEEAAIREVFEFVADDCDLDIRREGDASPAPAVETQNPD
                     ILNLLKTLEAEVQAQQAAASAPSPAQPAPERKAADLPPAAEKPAAAKAEAGKAAAAAN
                     AAQATIRVDIDRVDRLVDLIGELVINQAMLAQRVAEAGLGRGSAVATGLDELERLTRE
                     IQDSVMAIRAQPVKPVFQRMSRIVREVAEITGKSVHLITDGENTEVDKTVIERLADPL
                     THMIRNAIDHGIEEPAKRVAAGKPAEGVVRLSALHRSGRIVIEVADDGAGINRPRVKD
                     IAVRKGLVGPDAVLSDDEIDNLIFAPGFSTAESVSNISGRGVGMDVVKRSIQALGGRI
                     SISSRPGVGSTFTMSLPLTLAVLDGMVVSVAGQTLVLPLTAIVETLQPAETSVHSFGT
                     SGQVISVRKALTPLIDVGMRLGFRSAPADSLACVAILVESEGGGRSALLVDAIQDQRQ
                     IVVKSLEGNYGHVPGIAAATILGDGRVALILDVDTLVVAGQSISLEQPQAA"
     gene            complement(6217..6582)
                     /locus_tag="VKU03_05240"
     CDS             complement(6217..6582)
                     /locus_tag="VKU03_05240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004439870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ70699.1"
                     /translation="MSKTVLTVDDSRTMRQMLRAALVDAGFNVVQADDGVSGLEALEV
                     EKPDVIITDINMPRLDGFGFIEGVRSAPRHRAVPILVLTTESDAAKKERARSAGATGW
                     IVKPFDPDKLVSAIRRVTA"
     gene            complement(6579..6890)
                     /locus_tag="VKU03_05245"
     CDS             complement(6579..6890)
                     /locus_tag="VKU03_05245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HLJ70700.1"
                     /translation="MADVATAPAQSTITVALPAILDLAAAAPLTERLLAARGADLTID
                     ASEVLRIGGQCLQVLLSAAKTWSADRAAFEISQPSAEFAEALTRFGIDPGVLAMREPS
                     P"
     gene            complement(6890..8794)
                     /locus_tag="VKU03_05250"
     CDS             complement(6890..8794)
                     /locus_tag="VKU03_05250"
                     /inference="COORDINATES: protein motif:HMM:NF012245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLJ70701.1"
                     /translation="MAVVAMFALALIALVVTLTWMAGDSIYSARRDELKTVTQVAYKV
                     LEGQYQDFKNGKISEAEAQERAKAALRLMRYNASDYFFVQNDTGVTIVHGARPDQEGT
                     TTKVLAADGRLLSTALHDTARDNGDGFIGYSYAKPGEPLDHPSPKLSYVKWFAPWKWT
                     LGTGVYIDDVSAKIWDRVYVSGAIALGFLLAIGGFAGWIVLSLSRRLDALCGVMVALA
                     EGNNDVEVPAMEKDDEIGRMAKSVQYLKDAAIEKARVEAQAQADRRQAAEARDAHEAE
                     RSEEAKLLQQATEALGEGMARLARGDLAYRIATPFSPRIERLRTDFNNSVEKLQETLL
                     KISSTANVIKGGSQELSNAADDMSRRSEQQAASLEQTAAALDEITATVKKTAEGAARA
                     NELVSTAKSDAERSGDVVREATVAMGGIKNSSQQIGQIIGVVDEIAFQTNLLALNAGV
                     EAARAGEAGRGFAVVASEVRALAQRSAEAAKEIKTLISASGQQVEQGVDLVGRAGEAL
                     QRIAAQFAEVNTVVADIAVGAREQATGLEQINTAINEMDKGTQQGAAMAEQSRAATAN
                     LAQQTDELAHAVAQFRLDAAAESAKAEARRPPPPAPRAGVAKTALKTIVSRRDGAAVR
                     KPEADNWEDF"
     gene            complement(9347..>10701)
                     /locus_tag="VKU03_05255"
     CDS             complement(9347..>10701)
                     /locus_tag="VKU03_05255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019825 - oxygen binding [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0006935 - chemotaxis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="globin-coupled sensor protein"
                     /protein_id="HLJ70702.1"
                     /translation="IAAAEFGEGYSRGVREIGEANGRMGLEPRWVIGGYALIVERLVA
                     AIVKEQWPTLLRMRRARPDDVARTISSVMKAALLDIDLSLSVYLETLNELRCRAEEAG
                     KEAIGKERALVATSLGAAVAQLAAKDLTCRMSADLPEAYRELQADFNAAIGRLESAMR
                     GVNGAAESIQAGAQDIGAAVDDLARRTERQAARLEETAAALGQITAAVNASAESAGRA
                     RAVVGAADQDAKKSALVVRRTVESMDAIAQSAQQINKIIGAIDEIAFQTNLLALNAGV
                     EAARAGVAGKGFAVVASEVRALAQRSAEAAKDVKRLISASTAQVETGVRLVAETGASL
                     ERITSQVAEINGAVAAIAAGAKEQAAGLRQVNAAIAEMDDAIRENAAMAEQSTAASGA
                     LSQETNGLLDMIGEFSVGAEEPRQALPEPAPRAAARPARASGAAKAKGQKPLRAAAGR
                     "
BASE COUNT         1729 a         3554 c         3498 g         1920 t
ORIGIN      
        1 tatcaacgta ggagtccccg cagcgcggct gcggggattt cgtcaagggt aaggcttgcc
       61 ggccgtgcgc cctccgcgcc ggccatcgga tgtccgccag cgggcgacgc tccggacctg
      121 tccttctcgg tttccatcgc cgagatcaca atgttgagaa atactttaac gccgcaggcc
      181 tgtaccttcg tttatttctg cagttccgcg ccttcaaagc ggcaatttcc gattattcca
      241 ctcagccgca acggcgttaa aacaactcga actcgccatt ggcctcgtcg tcccgcgcag
      301 ccggcgctgc gggcgagcgc aaccgctcag caagcgcggc gagggtgacg ccctcggcgg
      361 cgggattggg gtcgagcgcc cattgcggcg gcgccgattg cgcgatggcg ttgaggaaat
      421 cggcgagaca ggtgagcttc tggtacgtgt ggtccagctc ctgcaacgct tgcgtcaaag
      481 gccggggcgg cgcgctcgtc gagtcatcgc cgtcggcgac cggaatcgca tcgacggcat
      541 gcgccgcatt ctgcagttca gccgccacgc gcctgagcag atcggcgagc ggctcggccg
      601 cttcgggggt cgaggctttg cgggccgctt tcgtcggcat cgtcgcctcg ccttagaaca
      661 gttcgatcgc gccggcggcg ctggcggcgc gcggcggcgg gggcggttcg aggatttctg
      721 ccggcgcctc gacgaaaagc tgccgggcct ggcccgatac cggcctgtac tccacccgcc
      781 gcccccgctc gtcgccgagg ctttcgctga cgatcttaat tccctcggtc gccaggaact
      841 tccgggcgaa ttccgcattc atgcggccga tcggcgacag gctcttgacc acgctggcgc
      901 cgccgaacag cttcgcctcc aggcgctgcc gactggcgcc gcgccgcaaa agcccgttga
      961 ccagaacctc catcaggtag gcgccataac gttcggcctc gctggagggc gataggcggc
     1021 tggcgtctcc cggcaaaaga aaatggttca tgccgccgac gccggctttc ggatcgcgaa
     1081 tgcaggcggc gacgcaagag ccgaggatcg tggtgaggac gacgtctgga tcgtccgaaa
     1141 cgtgacactc gccttgtatc acgcggatat atttcttgcc cgattgcctc aacatcacag
     1201 gcccttgatg atcgcttgca aggcggcccg caacgaggcg acggtgaacg gcttggtgac
     1261 gaaattgttg aggccgaatt ggcgcgcccg ctcgatcagg ctcttgtcgc cgcggccggt
     1321 gaccatcacg aagggcacgt gacgggtcgg ctgataggcg cgaatgccct gcagcaggcc
     1381 gacgccgtcc aggcccggca tctcatagtc ggaaatgacc agatgcgccg gcgtcgtcat
     1441 catgtgcttc aaagcctgct ggccattgct cgcataggcg atctggcgaa agccgatctc
     1501 ctgcagcgcg tcgatgacca acatacggct ggtggtcgtg tcgtcgacga cgagcacttt
     1561 gagttgctga gcgatggaca tcttaccctt tccccttgcg attcgtgacg gcgatgagtt
     1621 gatctcccat ccttgccaat gaaacttgct tctcgacggc gccgagctcg aaagccgctt
     1681 tcggcatgcc gtagacgacg ctcgtcgctt cgtcttggcc gagcgtactg gcgccggctt
     1741 cgcgcatggc cagcaggccg tgagcgccgt cttgtcccat gcccgtcagg atgacgccga
     1801 ccgcctttct acccattgct ttggcgatgg cgaagaacat cacgtcgacg gagggacgat
     1861 ggccattgac cggcgaagtc tctttgagac ggcagcgcgg atcgccgggc gggccgccga
     1921 tttcgagatg ggcgactgat ccgggcgcga gataaacttg gccgattttc agcggcgcgc
     1981 cttcataggc ctcagcgacg ctcggcgcgc atgagcggtt cagccgctcg gcgaggctct
     2041 tcgtgaagag cccgggaatg tgctgggtga tcaccgtcgg cgggcaattc gccgggaatt
     2101 tcgaaaggac gttcagcaag gcttcgacgc cgccggtcga ggagccgatc gcgacgacgc
     2161 ggccgtcggg ctcataagcc gcggcttggc cgcgcgtcga gccattggcc ggcgcgagac
     2221 gctgagacag cccgacgagc cgggccgacg cggccgcttt cactttcgcg gcgaggccgg
     2281 cgagcgaatg ttcgtcgccg ggcgaaggtt tggcgacgca gtcgaccgcg ccgagctcga
     2341 acgccctcac cgtgcattcg gcgccgcggc tcgtcaacgt cgagaccatg ataaccggcg
     2401 tcggccgcag ccgcatgatc ttttccagga acgacagacc gtccatttcc ggcatttcga
     2461 tgtcgagggt gacgacgtcc ggattgagcg ccttcatcgc ctcgcgcgct tcctgcgcat
     2521 tggccgcttc tccgacgacc tggatggacg ggtcgcgcaa cagctcggcg gagatcaggc
     2581 cgcgcatcgt gcgcgaatcg tcaacgacaa ggacgcgaac ggcgctcatc ggcgtgctcc
     2641 ctcgcacaag cgataggtcg tcacgccttc gcttcgaaaa gctgcggcgg ccggcccgga
     2701 cagccgctcc gaatggccga tatagagaac gccgccgggc gccagcgccg aggcgaagcg
     2761 gctccacagc ttggactgag tcggctggtc gaaatagatg acgacgttgc ggcagaagat
     2821 cgtttggaag ggattcttca tcggccaggg tccgatcaga ttaagctgcc gaaacgcgac
     2881 cagccggcgc gcctcctcgg cgacccgcca catctcgccg ttctcgccgc gcgccgggcg
     2941 aaaccagcgc tcgcgcaaat tggtcggcac gccagccagc gcgctcgccg aatagagtcc
     3001 gcgctcgccc gaagcgacca cattcggatc gatatcggtg gcgagaatct tcacgtcgta
     3061 attggcgata ttcggggcca gcgagagcag cgcgagcgca atcgaataag gctcctggcc
     3121 gctcgaacag gccgacgacc agaggcgcac gcgctcgccg cgctgcgccg ccgcgatcag
     3181 cgccgggccc atatgggctt tcagatgctc gaaatgatgc ggctcgcgga aaaacgccgt
     3241 gacattggtg gtcagcgccg cgatcatcct ttggcgttct tcgacgccgt catcgctggc
     3301 gatcaggctg caatagtcgc gaaagctcgc aaggcccaag gcgcgcaagc gcttggccag
     3361 gcgcgaatag accagcgccg tcttggactc gggaatgtag atgccggact cggcgtgcag
     3421 cgtcgccgcg atgtgcttga aatcgcggct cgacatttcg aactcgcttt gcgcgaaatc
     3481 ggcgtccttg tcttcggcat cgcggatcag cgccgagaag ttcaagcagc ctccctttcc
     3541 gcggctggaa cgacgccatc gagcgcgacg aagctgatca tccggccttc gatgacgaag
     3601 acgccgcgga cgaacgtcgc cgcgagatcg gacgcaatgt cgggcgtcgg ctgcacgaga
     3661 tcgtcggtga ccgtcagaat atcggacacc gcatcgacca gcaggccgac cgtacgctgg
     3721 ccgatctggg tgacgatgat gacgttctgc gcggtcggtt cggcgggcgg aaaaccgaac
     3781 cgcgcggcaa gatcgatgat cggcagcaca aagccgcgca gattgatcac gccgcgcacg
     3841 aaatcgggcg agcggggcag cggcgtcgcc ggcgtccagc agcggatttc gcgcaccgac
     3901 atgatgtcga cgcagaattc ctggttgccg atccgaaacg cgacgacttt gcgaggctcg
     3961 gccgcttcca tcgcttgagt ctggctcacg atgatcccct atgctgcttg aggctgttcg
     4021 agagaaatgg attggcccgc gacgacgagc gtgtcgacgt cgagaatcag cgcgacccgt
     4081 ccgtcgccga ggatcgtcgc ggccgcaatg ccgggcacat ggccgtaatt gccttcgagg
     4141 ctcttgacga cgatctgacg ctgatcctgg atcgcatcga cgagcagcgc gctgcgtccg
     4201 ccgccctccg attcgaccag gatcgcgacg caagcgagcg aatcggcggg cgcggagcgg
     4261 aagccgagcc gcatgccgac atcgatcaag ggcgtcagcg ctttcctgac cgagatgacc
     4321 tgacccgacg tgccgaaaga atggacgctc gtttcggccg gctggagtgt ctcgacaatc
     4381 gcggtcagcg gcaggaccaa ggtctggccg gcgacgctga ccaccatgcc gtcgagcacc
     4441 gccaaggtca gcggcaggct catggtgaag gtcgacccga cgcccgggcg cgaggatatg
     4501 ctgatccggc cgccgagcgc ctggatcgac cgcttgacca cgtccatgcc gacgccgcgg
     4561 ccggaaatgt tcgatacgga ttcggcggtc gaaaagccgg gcgcgaagat caggttgtcg
     4621 atctcgtcgt cgctgaggac ggcgtccggc cccacgagac ctttgcgtac cgcaatgtcc
     4681 ttgacgcgcg gccggttgat cccggcgccg tcgtcggcga cttcgatgac gatgcggccc
     4741 gagcgatgca gcgcgctgag gcgaacgacg ccttcggccg gcttgccggc cgcgaccctt
     4801 ttggccggct cctcgatgcc gtgatcgatg gcgttgcgga tcatatgggt gagcggatcc
     4861 gccaggcgct cgatgacggt cttgtccacc tcggtgttct cgccgtcggt gatcagatgt
     4921 accgacttgc cggtgatctc tgcgacttcg cggacgatgc gcgacatgcg ctgaaagacc
     4981 ggcttgaccg gctgggcgcg gatcgccatc acgctgtcct ggatttcgcg agtcaggcgc
     5041 tcgagttcgt cgaggccggt ggcgaccgcc gagccgcggc caaggccggc ttcggcgacg
     5101 cgctgggcga gcatcgcctg gttgatgacg agttcgccga tcaggtcgac cagccgatcg
     5161 acccggtcga tgtcgacgcg gatcgtcgcc tgcgcggcgt ttgcagcggc ggcggccttg
     5221 ccggcctccg ctttcgccgc ggcgggcttc tcggcggccg gcgggagatc ggccgccttg
     5281 cgctccggcg caggctgcgc cgggctcggc gccgaagcgg cggcctgctg cgcctgcact
     5341 tccgcctcga gcgtcttcag caggttgaga atatccggat tttgcgtctc gacggcgggc
     5401 gccggcgaag cgtcgccttc gcgacgaatg tcgagatcgc aatcatcggc gacgaactcg
     5461 aacacttcgc gaatcgccgc ttcctcgaca tcgcccggca acgtcaccgt ccagcgcagg
     5521 taggcgcctt ctgggtcgag atcgggcagg agcggcgttt cgccgatgtc gcagctcacc
     5581 acggcttcgc caagccgggc gagttcgcgg agcaagaggc ccgcttcgtt cgccttggcg
     5641 tagagggccg gtttcggcat gaactcgacg acatagcgcc ccgaagaagg cgcatcgccc
     5701 gccgccgcgg ctgcttcgtc gatggagttt gtatcgaacg agacctggac gggcgtgaaa
     5761 tcgaggccgt ccattccgtc gccgtcgtca ttggcggctg attcttccgc cgcgccgctc
     5821 aacgcgacca attcctcgat aatcccatcg ccgcgggcga tgtcggctgc gccgccgtcg
     5881 cgcgccgctc gcacgagatc ggccagcatg tcggccgcgc gcagcatgac cttggttccg
     5941 gcaggcgtcg ccgaaatctg cccggagcgc aaatgatcga gcgtcgtctc ataggcatgg
     6001 gcgaagcgca cgagatcgtc gagatggaac gcgccggcgc cgcccttgat cgaatggacg
     6061 gcgcggaaaa tcgcgttgac cgtttcgaga tccgcctcgc cgccttcgag cgcgagcagg
     6121 cccgcctcca attcggcgag ctgttcctcg cattcctgga agaatgtcgt cttgatgtcc
     6181 gcgattgcgt cggaagccat gacggagcct tgacccttaa gccgtgacgc ggcggatggc
     6241 gctgacgagc ttgtcgggat cgaacggctt gacgatccat cccgtcgcgc cggccgagcg
     6301 ggcccgctcc tttttggccg cgtcgctttc ggtggtcagt acgaggatcg gaacggcgcg
     6361 gtggcgcggc gcgctgcgca cgccctcgat gaagccgaat ccgtccaagc gcggcatatt
     6421 gatgtcggtg atgataacgt cgggcttctc gacttcgagc gcttccagcc cgctcacgcc
     6481 gtcatcggcc tggaccacat tgaagccggc gtcgaccaaa gccgcccgca gcatttggcg
     6541 catcgtgcgc gaatcatcca cggtcaacac agttttgctc atggcgacgg ctcccgcatg
     6601 gcgaggactc ctgggtcaat tccaaagcgt gtcagcgcct cggcgaactc cgccgagggc
     6661 tgcgagattt cgaacgccgc gcgatcggcg ctccaggttt tcgcggcgga gagcagcacc
     6721 tgcaggcact gtccgccgat gcgcaaaacc tccgaggcgt cgatcgtcag atcggcgccg
     6781 cgcgccgcca acaggcgctc cgtcagcgga gcggcggcgg cgagatcgag gattgccggc
     6841 agcgcgaccg taatcgtgga ttgcgccggc gccgtcgcca cgtcagccat cagaagtctt
     6901 cccaattgtc agcctcgggc ttgcgcacgg ccgcgccgtc gcgccggctg acgatcgtct
     6961 tgagcgcggt tttcgcaacg ccggcgcgcg gcgcgggcgg cggcggacgg cgcgcctccg
     7021 ccttcgccga ttccgcggcg gcgtcgagcc ggaattgggc gacggcatgc gccaattcgt
     7081 cggtttgctg cgcgagattg gcggtcgccg ctcgcgactg ctcggccatc gccgcgccct
     7141 gctgcgtgcc cttgtccatc tcgttgatcg ccgtattgat ctgctccagg ccggtcgcct
     7201 gctcgcgcgc gccgacggcg atgtcggcga cgaccgtatt gacctcggcg aactgcgccg
     7261 cgatgcgctg cagcgcctcc ccggcacggc cgacgagatc gacgccttgc tcgacttgct
     7321 ggcccgacgc cgagatcagc gtcttgatct ctttggccgc ttcggcggag cgctgcgcca
     7381 gcgcgcgcac ctcggacgcg accaccgcaa agccgcgacc ggcttcgccg gcccgcgccg
     7441 cctcgacgcc cgcattgagc gcaagcagat tcgtttggaa ggcgatctcg tcgacgacgc
     7501 cgatgatctg cccgatctgc tgcgacgagt tcttgatgcc gcccatcgcc accgtcgctt
     7561 cgcgcaccac gtcgccgctg cgctcagcgt cgctcttggc ggtcgagacc agctcgttgg
     7621 cgcgcgccgc gccttccgcc gttttcttca ccgtcgcggt gatctcgtcg agcgccgcgg
     7681 ctgtctgctc caggcttgcg gcttgttgct cgctgcggcg ggacatgtcg tcagccgcat
     7741 tcgagagctc ttgcgatccg cccttgatga cattggcggt cgaggagatt ttcagcagcg
     7801 tctcctgcaa cttttcgacg gagttgttga agtccgtgcg cagcctttcg attctcggcg
     7861 agaagggcgt ggcgattcga taagccaagt cgccgcgcgc caggcgcgcc atgccttcgc
     7921 cgagcgcttc ggtcgcctgc tggaggagct tggcctcctc cgatcgctcc gcttcgtgcg
     7981 catcgcgggc ttccgcggct tggcggcggt cggcttgcgc ctgcgcttcg actcgggcct
     8041 tctcgatcgc cgcgtccttg agatactgga cggacttcgc catccggccg atctcgtcgt
     8101 ccttctccat cgcggggact tcgacgtcgt tattcccctc cgccagcgcc accattacgc
     8161 cgcatagcgc gtcgagacgg cgagagaggc tcaggacgat ccaaccggca aagccgccaa
     8221 tcgccaggag aaagccgagg gcgatcgcgc ccgaaacata gacccggtcc cagattttgg
     8281 cgctgacgtc gtcgatatag acgccggtgc cgagcgtcca tttccacggc gcgaaccatt
     8341 tgacatagga gagtttcggc gagggatggt cgagcggctc gccgggcttg gcgtatgagt
     8401 agccgatgaa cccatcgcca ttgtcgcgcg ccgtgtcatg cagcgccgtc gacagcaggc
     8461 ggccgtccgc ggccagcacc ttggtcgtcg tcccttcctg gtccggccgt gcgccgtgca
     8521 cgatcgtgac gccggtatcg ttctggacaa agaaatagtc gctggcgttg tacctcatca
     8581 agcgcagcgc cgccttggcc cgctcttgcg cctcagcctc ggaaatcttg ccgttcttga
     8641 agtcctggta ctgaccttcg aggactttat acgcgacctg ggtcacagtc ttgagttcat
     8701 cacgacgagc cgaatagatc gaatcgccgg ccatccaagt cagcgtgacg accagggcga
     8761 tcagcgcgag cgcgaacatc gcaacgacgg ccatcaacct ggcgcgaatc gacgtgaaaa
     8821 aacctgcgcc aaagcgtcca acatccgtgg gcgtcattgt ccttgcttcc ttcgattgcc
     8881 aattgttctt caataagcga gagcgcagct cttcgggccg catcagacgg atttcgagga
     8941 ttgtctccgc cggccgtccg aaaggcgctg cgaaagcgcg gcgcgcttgt gatttcgcct
     9001 gaaagtccgt cgagaaacat ggagcggcga agcccggggc agccctaggc tcggactcga
     9061 gcggaagcgc ggcatgaaga caccctgaaa gacgcgggag gcgttgatca acatccggat
     9121 tgaaccggcg cgacgagacg gcttcatgcc ttcggaatgc gcaatgattg cgcgcccaac
     9181 ccctcgtgtt cgcaagtgtg cagttatttc cttacgaatg tgttagcgat gacgccgcgc
     9241 ggcatccgtc ttcttgcgga ttccaaatgg ctatccggcc tggggccgga ggcgcaaagg
     9301 cgacgtactc cggcctgaag aggcgtcttt ttcgcggacg cctcggtcag cggccagccg
     9361 ccgccctgag cggtttttgc cccttcgcct tcgcagcgcc ggaggctctc gccggccggg
     9421 cggcggcgcg cggcgcgggt tccggcaagg cttggcgcgg ctcttcggcc ccaaccgaaa
     9481 attctccgat catgtcgagg agtccgttcg tctcctgcga caacgcgccg ctggccgcgg
     9541 tcgactgctc tgccatggcg gcgttctcgc gaatcgcatc gtccatctcg gcgatcgcgg
     9601 cgttgacctg ccgcaagccg gccgcctgtt ctttcgcgcc ggccgcgatc gccgcgacgg
     9661 cgccgttgat ctcggcgacc tgactggtga tccgctcgag cgaggcgccg gtctcggcga
     9721 caagcctcac tccggtttcg acctgcgcgg tcgaagccga gatcagccgc ttgacgtctt
     9781 tcgccgcctc cgccgagcgc tgcgccagcg cccgcacttc cgaggcgacg acggcgaagc
     9841 ccttgcccgc gacgccggcg cgcgccgcct caaccccggc attgagggcg agcagattgg
     9901 tctggaaggc gatctcgtcg atcgcgccga tgatcttgtt gatctgctgc gccgactggg
     9961 cgatcgcgtc catcgattcg accgtccggc gaacgacaag cgcgctcttc ttggcgtcct
    10021 gatctgcggc gccgacgacc gcgcgggcgc ggccggcgct ttcggcggat gcgttcaccg
    10081 cggcggtgat ttggccgagc gcggcggccg tctcttcgag gcgcgcggcc tgccgctccg
    10141 tacggcgcgc gagatcgtcg acggcggcgc cgatgtcttg cgcgccggcc tggatggatt
    10201 cggcggcgcc gttcacccct cgcatggcgc tttcgaggcg cccgatcgcg gcgttgaagt
    10261 ccgcctgcaa ttcgcgatag gcctcgggaa gatcggcgct catgcggcag gtcaggtcct
    10321 tggcggcgag ttgcgccacg gccgcgccga gcgatgtcgc gaccagagcg cgctctttgc
    10381 cgatcgcctc cttgcccgcc tcttccgccc ggcagcgcag ttcgttcaat gtctccagat
    10441 agaccgacag gctcagatcg atatcgagca gggcggcctt catcacgctc gaaatggtcc
    10501 gcgcgacgtc gtccggcctg gcccggcgca tccggagcaa agtcggccat tgctccttga
    10561 cgatcgcggc gaccagccgc tcgacgatca gggcgtaacc gccgatcacc cagcgcggtt
    10621 cgaggcccat gcggccattg gcctcgccga tctcgcgtac gccgcgcgaa tagccttccc
    10681 cgaactcggc cgccgcgatc g
//
LOCUS       DATGJW010000175         2132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_31749, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000175 DATGJW010000000
VERSION     DATGJW010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2132
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31749"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /gene="cbiT"
                     /locus_tag="VKU03_05260"
     CDS             <1..487
                     /gene="cbiT"
                     /locus_tag="VKU03_05260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="precorrin-6Y C5,15-methyltransferase
                     (decarboxylating) subunit CbiT"
                     /protein_id="HLJ70703.1"
                     /translation="ALTLAALAPWPGARLWDIGAGSGSIAIEWMLAHPRNRAVAFEKR
                     TDRAARIARNAAALGAPDLAVLAGDARDVMRDLPPPDAIFVGGGGAAMIDAAWSHLPA
                     GRRFVVNAVTIETQAAVVERQARHGGDLVQIAVAHPAPVGSYHAWRPAAPIVQWAATK
                     R"
     gene            466..864
                     /locus_tag="VKU03_05265"
     CDS             466..864
                     /locus_tag="VKU03_05265"
                     /inference="COORDINATES: protein motif:HMM:NF014006.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein"
                     /protein_id="HLJ70704.1"
                     /translation="MGGDEAMIAIGIGCRRGASQAAITTMIERALALAQRLGESVRLF
                     SIEAKSGEAGLVGAAQAMGLPLEFLPADALRAVEGKIATPSPQAKATLGVASVSEAAA
                     LAGAGRDARLVVKRMIGDGVACAVARGSGR"
     gene            861..1622
                     /gene="cobM"
                     /locus_tag="VKU03_05270"
     CDS             861..1622
                     /gene="cobM"
                     /locus_tag="VKU03_05270"
                     /EC_number="2.1.1.133"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0046026 - precorrin-4
                     C11-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-4 C(11)-methyltransferase"
                     /protein_id="HLJ70705.1"
                     /translation="MTIHFIGAGPGAPDLITLRGRDLIARSPVCLYAGSLVPKELLAF
                     CPPGARIVDTAPLALEAIVEEFRRAYAVGQDVARLHSGDLSLWSAMGEQLRELDKLGI
                     PYDVTPGVPSFAAAAAALRRELTLPALAQSLVLTRTGGRASPMPERETLANFAATGAT
                     LAIHLSIHALGRVVAELAPFYGENCPAAVIYRASWPDERVIEATLGTIAAQVGEAAIE
                     RTALILVGPALAARDFRASALYDADYQRRFRGRDQ"
     gene            complement(1616..>2132)
                     /locus_tag="VKU03_05275"
     CDS             complement(1616..>2132)
                     /locus_tag="VKU03_05275"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020493751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HLJ70706.1"
                     /translation="RGVDVARACGLGHLAGATGSTSEQAVKRLHALPEEALIDMGDFV
                     GGFLKYLRKHPVETVSIAGGFAKMTKLGQGLLDLHSRRGEVDLVWLAALAASAGLGEE
                     LAARIRAANTAMEAHALAREAGFGLAAGVAEAAWRTAAKAAERGAEKLEIVVVGRDGA
                     ILATTGIRPLH"
BASE COUNT          288 a          770 c          746 g          328 t
ORIGIN      
        1 cgccctcacg ctcgccgcct tggcgccgtg gcctggcgcg cggctctggg acattggcgc
       61 cggctcaggg tcgatcgcca tcgagtggat gctggctcat ccgcgcaatc gcgccgtggc
      121 gttcgagaag cggacggatc gcgcagcgcg gatcgcgcgc aacgccgcgg ctttgggcgc
      181 gcccgacctc gctgttctcg ccggcgacgc gcgcgacgtg atgcgcgatc tgccgccgcc
      241 ggatgcgatt ttcgtcggcg gcggcggggc ggcgatgatc gacgccgcct ggagtcattt
      301 gccggcgggc cggcgcttcg tcgtcaacgc cgtcacgatc gagacgcagg ccgcggtggt
      361 cgagcgccag gcgcgccacg gcggcgatct cgtgcagatc gccgtcgccc atcccgcgcc
      421 cgtcggctcg taccatgctt ggcggccggc cgcgccgatc gtgcaatggg cggcgacgaa
      481 gcgatgatcg cgatcggaat cggctgccgt cgcggcgcct cgcaagcggc gatcacgacg
      541 atgatcgagc gggcgctggc gctggcgcag aggcttggcg aatccgtgcg gcttttcagc
      601 atcgaggcga aaagcggcga ggccgggctc gtcggcgccg cgcaggcgat gggcctgccg
      661 ctcgaatttc tgcccgccga cgctttgcgc gcggtcgagg gcaagatcgc gaccccttcg
      721 ccgcaggcga aggcgacgct cggcgtcgcc tcggtttccg aggccgcggc gctggccggc
      781 gccgggcgag acgcgcgcct cgtcgtaaag cgcatgatcg gcgacggcgt cgcctgcgcc
      841 gtcgcccgag ggagcggccg atgacgatcc atttcatcgg cgccggtccc ggggcgccgg
      901 acctcatcac tttgcgcggc cgcgacctca tcgcccgctc gcccgtctgt ctctacgccg
      961 gctcgctcgt gccgaaggaa ttgctcgcct tctgtccgcc cggcgcgcgg atcgtcgaca
     1021 ccgccccgct cgcgctcgaa gccatcgtcg aggaattccg ccgcgcttat gccgtgggcc
     1081 aggacgtggc gcggctgcat tccggcgatc tcagcctatg gagcgcgatg ggcgagcaat
     1141 tgcgcgaatt ggacaagctc ggcattccgt acgacgtgac cccgggcgtg ccgagcttcg
     1201 ccgccgccgc ggcggcgttg agacgcgagc tgacgctgcc ggcgctcgcg caatcgctcg
     1261 tcctcacccg caccggcggg cgcgcctcgc caatgccgga gcgagagacg ctcgccaatt
     1321 tcgcggcgac cggcgccacg ctcgcaattc atctctccat ccacgcgctc ggacgcgtcg
     1381 tcgccgagct tgcgcctttc tatggcgaaa actgcccagc cgctgtcatc tatcgcgcga
     1441 gctggccgga cgagcgcgtc atcgaagcga ccctcggtac gatcgccgcg caggtcggcg
     1501 aagccgcgat cgagcgcacc gcgctgattc tggtcggccc ggctctggcg gcgcgcgatt
     1561 tccgcgccag cgcgctttac gacgccgact atcagcggcg ctttcgcggc cgcgatcaat
     1621 gaagcggccg gattccagtc gtcgcgagga tggcgccgtc gcggccgacg acgacgattt
     1681 cgagcttttc cgcgccgcgc tcggccgcct tggcggcggt gcgccaggcc gcctcggcga
     1741 cgcccgcggc gaggccgaag ccggcctccc tggccagcgc atgggcctcc atcgcggtat
     1801 tggcggcgcg aatgcgcgcc gcgagctctt cgccaagccc cgcgcttgcg gcgagcgccg
     1861 ccagccaaac gagatcgacc tcgccgcgcc gcgaatgcag atcgagcagg ccttggccga
     1921 gctttgtcat cttggcgaag ccgccggcga tactgactgt ctcgactgga tgcttgcgca
     1981 gatatttcag gaagccgccg acgaaatcgc ccatgtcgat cagcgcctct tccggcagag
     2041 cgtgaagccg cttgaccgcc tgctccgagg tcgaaccggt cgcgccggca agatggccga
     2101 ggccgcaggc gcgggcaacg tcgacaccgc ga
//
LOCUS       DATGJW010000176         2295 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_863264, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000176 DATGJW010000000
VERSION     DATGJW010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2295)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2295)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2295
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_863264"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..123
                     /locus_tag="VKU03_05280"
     CDS             <1..123
                     /locus_tag="VKU03_05280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HLJ70707.1"
                     /translation="MIAWAGLERLRLGLADDLAAPARPRWPLDAKAAPALNGKA"
     gene            252..1247
                     /locus_tag="VKU03_05285"
     CDS             252..1247
                     /locus_tag="VKU03_05285"
                     /EC_number="1.1.1.94"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012040639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0047952 - glycerol-3-phosphate
                     dehydrogenase [NAD(P)+] activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046167 - glycerol-3-phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent glycerol-3-phosphate
                     dehydrogenase"
                     /protein_id="HLJ70708.1"
                     /translation="MTEFAKIGIAGAGAWGIALANVAASAGRRVVLWGRDDAEMLSLA
                     RTRESPRRLPNVFLAESVKATSEIVDLADCQAALIVVPAQATRSVAAELAEILTPERP
                     LVACAKGVEQGEDLFMTQVLKAAAPNHPAAILSGPSFAADVAAGLPTAVTLAALDENL
                     AAALAGALQSASFRLYHSTDVIGVEIGGAAKNVLAIASGVAAGLNLGASAGAALIARG
                     FAELSRLGAALGAKPATLAGLSGLGDLVLTCTSPQSRNFAFGLALGRGAAVSQALATS
                     KLAEGVYTAGALIEIAEAHGVDMPIAASVEALLKGAIDPRAAVRALLARPPKAEV"
     gene            1628..2041
                     /locus_tag="VKU03_05290"
     CDS             1628..2041
                     /locus_tag="VKU03_05290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009493637.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HLJ70709.1"
                     /translation="MAHWLVKSEPSSWSFDQQLAAGAKGTNWNGVRNHAAKLNLMAMK
                     LGDQAFFYHSNEGKEIVGIVEVIKTYYPDPTDESGKFGMVDFRAVKRLPKPVTLEAIK
                     ADKRLANMALVKLSRLSVQPVTDAEWKIVSAMGGL"
     gene            complement(2077..>2295)
                     /locus_tag="VKU03_05295"
     CDS             complement(2077..>2295)
                     /locus_tag="VKU03_05295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70710.1"
                     /translation="AGFMDKVRATRARFVKGSSPPELVADAIFRAANDPSDRLRYLVG
                     PDVKRIWPVRRWLGSRTQMRIMRRVFGL"
BASE COUNT          369 a          771 c          767 g          388 t
ORIGIN      
        1 atgatcgcct gggctggcct cgagcggctg cgcctcggcc tcgccgacga tctcgccgcc
       61 ccggcgcggc cgcgctggcc gctcgacgcc aaagcagcgc ccgctttgaa cggcaaggcg
      121 tagatgaccg ggggcgcttc cctcatgtca ttcctgccca agcgggaatc cagagagccg
      181 ccgcgcctga gagctggcgc cgcctggctt tccgcttgct tgggaacgac acaggcaaat
      241 ggaggcccag cctgacagaa ttcgcgaaga tcggaatcgc cggcgccggc gcctggggca
      301 tcgccctcgc caatgtcgcg gcgagcgccg ggcggcgcgt cgtactgtgg ggccgcgacg
      361 acgcggagat gctgtctctc gccaggacgc gcgaaagtcc gcgtcggctt ccgaacgtgt
      421 ttctcgccga gagcgtcaag gcgacatccg agattgtcga tctcgccgat tgtcaggccg
      481 cgctgatcgt cgtgcctgcc caggcgacgc gctcggtcgc cgccgaactt gccgagattc
      541 tcacgcctga acggccgctc gtcgcctgcg ccaagggcgt cgaacaaggc gaagatctgt
      601 tcatgacgca ggttctcaag gcggctgcgc cgaaccatcc cgccgcgatt ctgtccggcc
      661 cgagtttcgc cgccgatgtc gccgccggcc tgccgaccgc cgttaccctc gcggcgttgg
      721 acgaaaatct cgccgcggcg ctggcgggcg cgctgcaaag cgcgagcttc cggctgtatc
      781 attcgaccga cgtgatcggc gtcgagatcg gcggcgcggc gaagaacgtg ctcgcgatcg
      841 ccagcggcgt ggctgcgggc ttaaatctcg gcgcgagcgc cggcgccgct ttgatcgcgc
      901 gcggcttcgc cgaattgtcg cgcctcggcg cggcgcttgg cgccaaaccc gcgactctcg
      961 ccggcctttc cgggcttggc gatctcgtcc ttacttgcac ttcgccgcaa tcgcgcaatt
     1021 tcgccttcgg actggcgctc ggccgcggcg cggccgtttc gcaagcgctg gcgacgagca
     1081 agctcgccga aggcgtctat actgcgggcg ctttgatcga gatcgccgag gcgcatggcg
     1141 tcgacatgcc gatcgccgca agcgtcgaag ccttgctgaa aggggcgatc gatccccgcg
     1201 ccgcggtccg cgcgctgctc gcccgtccgc cgaaggcgga agtctaaaag cccggcgaaa
     1261 aagccctttt ctgcaacgca ggcgagtctc cttccgccgg attgcaattt gcggttggcc
     1321 ttttgccctt aggcgcgggc ggcgccggcg gacctgattc agccgacgcc ggaagtggcg
     1381 gcgcgcacga cacgggattt tgtccgcggc gtgatcgccg tggacggtcg gacgatcagc
     1441 atgatcgaac tcgacgccgt gatcccggac cgcgccgagg cgcaggcggc ttagccctct
     1501 cggcggccgc ggagttgcag cggggcggca ggctcgccgc gcgcctttac cctcccgccg
     1561 ccttcgctta ggctgaatcg gccttgtgtg gcgccagggc ggggcgcgcc gcttgcgtga
     1621 ggggaacatg gctcactggc tcgtcaaaag cgaaccctcg tcctggtcgt tcgaccagca
     1681 actcgccgct ggcgcgaaag ggacgaactg gaacggcgtg cgcaatcacg cggccaagct
     1741 caacctgatg gcgatgaagc tcggcgatca ggccttcttc tatcattcga acgaaggcaa
     1801 ggagatcgtc ggcattgtcg aggtgatcaa gacctattat cccgatccga cggacgagag
     1861 cggaaagttt ggcatggtcg acttccgcgc cgtgaaaagg ctgccgaagc cggtgacgct
     1921 cgaagcaatc aaggccgaca agcgcctcgc caatatggcc ttggtcaagc tgtcgcggct
     1981 ctcggtgcag ccggtgaccg acgccgagtg gaaaattgtc tcagcgatgg gcgggctctg
     2041 atctggtcat tcccgcttcg cgggaatgac aaggcgtcac agtccgaaca ccctgcgcat
     2101 gatcctcatt tgcgtgcgag agccaagcca gcggcgcacc ggccagatgc gcttgacgtc
     2161 cggtccgacg agatagcgca ggcggtcgct cggatcgttc gccgctcgga aaatcgcatc
     2221 ggcgacgagc tccggcgggc tcgaaccctt gacgaagcgc gcgcgcgtcg ccctcacctt
     2281 gtccatgaag ccggc
//
LOCUS       DATGJW010000177         2070 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1278196, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000177 DATGJW010000000
VERSION     DATGJW010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2070)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2070)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2070
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278196"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /gene="tgt"
                     /locus_tag="VKU03_05300"
     CDS             complement(<1..907)
                     /gene="tgt"
                     /locus_tag="VKU03_05300"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HLJ70711.1"
                     /translation="MSEAFSFTIDARDGAARRGLVSTPRGAIRTPAFMPVGTAASVKA
                     MYPGEVRALGADILLCNTYHLMLRPSAERIAALGGLHKFMNWPRPILTDSGGFQVMSL
                     AKLRKLDESGVVFQSHIDGARHALTPERAMEIQRLLGSDIQMQLDECVRLPCSDEEAA
                     RAMRLSLRWAERSKAAFGAQPGRAIFGIVQGGVDAELRRESARELVSLGFDGYAIGGL
                     AVGEPQAEMLATIETVMPDLPADRPRYLMGVGSPDDLLQALSRGVDMFDCVMPTRAGR
                     HGMVFTRHGRMNLRNARFADDPRPID"
     gene            complement(904..1992)
                     /gene="queA"
                     /locus_tag="VKU03_05305"
     CDS             complement(904..1992)
                     /gene="queA"
                     /locus_tag="VKU03_05305"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HLJ70712.1"
                     /translation="MRVDQFDFDLPPDRIALRPANPRDSARMLVVALDGALADRGVAD
                     LPDYLRQGDALVVNDTKVIAARLDGIRVRGEAVAHIEATLIKRLDGSRWRALVRPAKK
                     LDVGERIRFGEDAESMVCLLGALDAEVEEKGEGGEVTLAFAFHGAALDEAIERLGQAP
                     LPPYIAARRPPSDADRRDYQTIFAEEAGAVAAPTAGLHFTDDLIARVQAKGATVHRVT
                     LHVGAGTFLPVKGEDTDSHQMHAEWGRIDEATAAALNEARASGGRIIAVGTTSLRLLE
                     SAAGEEGRIAPFSGETSIFITPGYRFRAVDALLTNFHLPRSTLFMLVSAFSGLDVMKR
                     AYAHAIAAGYRFYSYGDACLLLRRGEWA"
BASE COUNT          338 a          706 c          724 g          302 t
ORIGIN      
        1 catcgatcgg ccgcggatcg tcggcgaagc gcgcgttgcg cagattcatc cgcccgtgtc
       61 gtgtgaagac catgccatgg cgcccggcgc gggtcggcat cacgcaatcg aacatgtcga
      121 cgccgcgcga cagcgcctgc agcagatcgt cgggcgagcc gacacccatc aggtagcgcg
      181 gccggtcggc cggcaggtct ggcatgacgg tctcgatcgt cgccagcatc tcggcctggg
      241 gctcgccgac cgccaacccg ccaatcgcgt aaccgtcgaa gccgaggctg accaattcgc
      301 gcgcgctctc gcgtctgagt tcggcgtcga cgccgccctg cacgatgccg aagatcgccc
      361 gccccggctg cgcgccgaaa gccgccttcg agcgctcggc ccagcgcagc gacaggcgca
      421 tcgcccgcgc cgcctcttcg tccgaacagg gcagccgcac gcattcgtcg agctgcatct
      481 ggatgtccga gccgagcagg cgctggatct ccatcgcgcg ctccggcgtc agcgcatgcc
      541 gcgcgccgtc gatatgcgat tggaagacga cgccgctttc gtcgagcttg cgcagcttgg
      601 cgagcgacat gacctggaag ccgccggaat cggtgagaat cggacgcggc cagttcatga
      661 atttgtgcag gccgccgagc gcggcgatcc gctcggcgct cggccgcagc atcagatgat
      721 aggtgttgca gagcaggatg tcggcgccga gcgcgcggac ctcgcctggg tacatcgcct
      781 tgacgctcgc cgccgttccg accggcatga aggctggagt gcggatcgcg ccgcgcggcg
      841 tcgaaacgag cccgcgccgc gccgcgccgt cgcgcgcgtc gatcgtgaaa gagaaggcct
      901 cgctcacgcc cattcgcctc ggcgcaggag caggcaggcg tcgccatagc tgtaaaagcg
      961 gtagccggcc gcgatcgcat gggcgtaagc ccgcttcatg acgtcaaggc cgctgaaggc
     1021 cgagaccagc atgaacagag tcgagcgcgg caggtggaaa ttggtgagca gcgcgtcgac
     1081 ggcgcggaag cgatagccgg gcgtgatgaa gatcgaggtt tcgcccgaga aaggcgcgat
     1141 gcgcccttcc tcgccggccg cgctctcgag caggcgcagg ctggtcgtgc cgaccgcaat
     1201 aatgcgcccg ccggaggcgc gcgcttcgtt gagcgccgcc gcggtcgctt catcgatccg
     1261 cccccattcg gcgtgcatct gatgcgagtc cgtatcctcg cctttgaccg gcaagaacgt
     1321 gccggcgccg acatgcagcg tcacgcgatg gaccgtcgcg cccttggctt gaacgcgggc
     1381 gatgagatcg tcggtgaaat gcaagcccgc ggtcggcgcg gcgacggcgc cggcctcctc
     1441 ggcgaaaatc gtctggtagt cgcgccggtc ggcgtcgctc ggcggccgcc gcgccgcgat
     1501 gtaaggcggc agcggcgctt ggcccaagcg ctcgatcgcc tcgtcgagcg cggcgccgtg
     1561 aaaagcgaaa gcgagcgtga cctcgccgcc ctcgcctttc tcctccacct cggcgtcgag
     1621 cgcgccgagc aggcaaacca tgctctcggc gtcctcgccg aagcggatgc gctcgccgac
     1681 atcgagcttc ttggccggcc ggacgagagc gcgccagcgg ctgccgtcga ggcgcttgat
     1741 cagcgtcgcc tcgatatggg cgacggcctc gccgcgaacc ctgatcccgt cgagccgcgc
     1801 cgcgatgacc ttggtgtcgt tgacgacgag cgcgtcgccc tgtcgcagat aatccggcag
     1861 gtcggcgacg ccgcgatcgg cgagcgcgcc gtccagcgcg acgacgagca tgcgcgcgga
     1921 atcgcgcgga ttcgccggcc gaagcgcgat gcggtcgggc ggcagatcga aatcgaattg
     1981 gtcgacgcgc atggggaagt acgatggaag gcggaaaaac ccttctctcc cgtgaaacgg
     2041 ggagaaggtg gcccgaaggg ccggatgtgg
//
LOCUS       DATGJW010000178         5553 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1172041, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000178 DATGJW010000000
VERSION     DATGJW010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5553)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5553)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5553
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172041"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..405
                     /locus_tag="VKU03_05310"
     CDS             <1..405
                     /locus_tag="VKU03_05310"
                     /EC_number="2.3.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604005.1"
                     /note="FabB, beta-Ketoacyl-ACP synthase I, KASI; catalyzes
                     a condensation reaction in fatty acid biosynthesis:
                     addition of an acyl acceptor of two carbons from
                     malonyl-ACP; required for the elongation of short-chain
                     unsaturated acyl-ACP; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase I"
                     /protein_id="HLJ70713.1"
                     /translation="GAVRCMRQALATVKAPIDYINPHATSTPVGDEKEIEAIRQVFGS
                     GDKCPPIAATKSLTGHSLGATGVQEAIYSLLMMNNGFICESAHIEELDPAFADMPIVR
                     QRRDNVTIGAVASNSFGFGGTNATIVLKHVDA"
     gene            605..1441
                     /gene="fabI"
                     /locus_tag="VKU03_05315"
     CDS             605..1441
                     /gene="fabI"
                     /locus_tag="VKU03_05315"
                     /EC_number="1.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011995731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase FabI"
                     /protein_id="HLJ70714.1"
                     /translation="MSAARAGLMAGKRGLVMGVANDHSIAWGIAKALAEHSAELAFTY
                     QGEALGKRVRPLAESLGSKLALPCDVENLASVDQVFAALKKSWGAIDFAVHCIAYSDR
                     GELKGRYADTTRENFTRTMLISAFSFTEIAKRAAALMPKGGALLTLTFGGATRVMPNY
                     NVMGVAKAALEASVRYLAADFGPAGVRVNAISAGPVRTLAGAGIADARFMFNFEREHS
                     PLRRPITIEDVGGAALYLLSPLSSGVTGEIHFVDSGYNIISMPHPDDIKAAKGERGLA
                     AE"
     gene            1619..2839
                     /locus_tag="VKU03_05320"
     CDS             1619..2839
                     /locus_tag="VKU03_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic murein transglycosylase"
                     /protein_id="HLJ70715.1"
                     /translation="MIRWLLRSLAVLSLCVLAAASASAHAKIEKKSERAFRAFIAKLW
                     PEAEAHGVSRGTFDRAFRGVTFDPKIIALTRRQAEVIKPIWGYIASAVSPTRVAQGED
                     KAENYRAWLARAQQKYGVDPAIIIGIWGLETDFGGFAGNDNVIRCLATLAFAHYRDDY
                     FKDELIAALEILEEGDIAPGRMKGSWAGAMGQTQFMPSSFLKYAVDFEGHGRRDIWTS
                     APDAIGSTANYLKEHGWRPGEPWGFEVALPQDFALRAADALDYAPFSAFARRGVTRAD
                     GRPLPETGEAQLLIPAGLKGPIFLVTPNFKVIKSYNNSTSYALAVALLGDRAMGGAEL
                     VGAWPVNDRRLTLAQARDMQIRLKKMGYDVGKIDGKFGETGQTALRAYQERNGLTPDG
                     YPTLALLERMRKRP"
     gene            2866..4092
                     /locus_tag="VKU03_05325"
     CDS             2866..4092
                     /locus_tag="VKU03_05325"
                     /inference="COORDINATES: protein motif:HMM:NF016218.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH family hydrolase"
                     /protein_id="HLJ70716.1"
                     /translation="MIALGPLLRKLAGSRAVKAIAAAVFLSMAGGPAVMASSDNSQTF
                     WIEQRQLNEQRQKAREQRAPAPAPKARQPHDFVPTETTRAGGADSGPVQPTFFVDVIG
                     DSLALLTADGLREAFADNPKITISSKARDSSGLVRDDFYDWVKAAHDLVAERSNKNRI
                     DLAVMEIGLNDMQAIKEGGDSFDPLSDRWRDIYGKRVEAVAEAFRQAHIPLLWIGLPP
                     MKNDKFNDQMSKLNELIKDHAEKAGAKYIDIWNAFADENGAYAAFGPDVNGRIVRLRI
                     ADGVYYTKAGARKAAQFVEGDIRRAFDAAKPADDMVDLPPDVEQATIDINAEIRREMG
                     LAPNSSEVAPPEADKPLAGPVLNLTARPVSPGGALLSRSPWSNGGDAALNTLASGVEP
                     PAKAGRADDFAWPAKP"
     gene            4194..4685
                     /gene="secB"
                     /locus_tag="VKU03_05330"
     CDS             4194..4685
                     /gene="secB"
                     /locus_tag="VKU03_05330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0015031 - protein transport [Evidence IEA];
                     GO_process: GO:0051262 - protein tetramerization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-export chaperone SecB"
                     /protein_id="HLJ70717.1"
                     /translation="MADQNGGAAPEPANAGPRLSVLVQYTKDFSFENPNAPRSFGPQQ
                     QQPNISIQVNVNARQIGPTDYESVLVLEGGAGEGANTLFKFELNYAGVFRIENIPAEQ
                     IQHVVMVEGPRLLFPFARQIIADAVRSGGYPPLYVDPIDFNALYFQRMAAANATAPTA
                     GVA"
     gene            complement(4698..5432)
                     /gene="dnaQ"
                     /locus_tag="VKU03_05335"
     CDS             complement(4698..5432)
                     /gene="dnaQ"
                     /locus_tag="VKU03_05335"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit epsilon"
                     /protein_id="HLJ70718.1"
                     /translation="MREIVLDTETTGLDPAEGHRMVEIGCIEIVHQIPTGAVFHTLIN
                     PERDVPDDAFRVHGHSTEKLSRQPLFADVAPDFLAFIGDDPLVIHNAEFDIKFINAEL
                     ARLGAEPIAPSRIVDTLAIARRKHPGAPNNLDALCDRYHIDRSRRVRHGALLDAEILV
                     EVYGELLGGRQKSLVFAAPRAEARAAARAVNCAIDRARIVGPLITDAERAAHARYIES
                     FGEHAIWRRYLAEPPLPAAASREAVG"
BASE COUNT         1013 a         1781 c         1799 g          960 t
ORIGIN      
        1 ggcgccgtgc gctgcatgcg ccaggcgctg gcgacggtga aagcgccgat cgactatatc
       61 aacccgcacg ccacctcgac cccggtcggc gacgaaaaag agatcgaggc gatccgccaa
      121 gtgttcggct cgggcgacaa atgcccgccg atcgcggcga ccaaatcgct gaccggccat
      181 tcgctcggcg cgacgggcgt gcaggaggcg atctattctc tgctgatgat gaataacggc
      241 ttcatctgcg aaagcgctca tatcgaggag ctcgatccgg ctttcgccga catgccgatc
      301 gtccggcaaa ggcgcgacaa tgtgacgatc ggcgcggtcg cttcgaactc cttcggcttc
      361 ggcggcacca atgcgacgat cgtgctcaag catgtcgacg cgtgaggggc ggtttttcgt
      421 tgatgaaggt gcgcggctta ccctcacccc aaccctctcc cgcaagcggg agagggggcg
      481 cgccgagctt gagccagcca caattccctt ctcccgcttg cgggagaagg tggcccgaag
      541 ggccggatga gggtaagccg cggctgcccg cgccggcgct tttggttttt cgaagggagc
      601 gacaatgagc gccgccaggg cgggtttgat ggccggcaag cgcggcctgg tcatgggcgt
      661 cgccaatgat cactcgattg cctggggcat cgccaaggct ttggccgagc atagcgccga
      721 gcttgccttc acttatcagg gcgaagcgct cggcaagcgg gtccggccgc tcgccgagag
      781 cctaggctcc aagctcgccc tgccctgcga tgtggagaat ttggcctcgg tcgatcaggt
      841 cttcgcggcg ctgaaaaaaa gctggggcgc catcgacttc gccgttcatt gcatcgccta
      901 ttcggaccgc ggcgaattga agggccgcta cgccgacacg actcgggaga atttcacccg
      961 cacgatgctg atctccgcct tctccttcac cgagatcgcc aagcgcgccg cagcgttgat
     1021 gccgaaaggc ggcgcgttgc tgaccctgac ctttggcggc gcgactcggg tcatgccgaa
     1081 ctacaatgtg atgggcgtcg ccaaagcggc gctggaggcg agcgtacgct atcttgccgc
     1141 cgatttcggc cccgctggcg tgcgcgtcaa cgcgatctcg gccggcccgg tgcgcacgtt
     1201 ggcgggggcc ggaatcgccg acgcccgctt catgttcaat ttcgagcgcg agcattcgcc
     1261 gctgcgccgg ccgatcacga tcgaagatgt cggcggcgcg gcgctctacc ttttgtcgcc
     1321 cttatcgtcc ggcgtgaccg gcgagattca cttcgtcgat tcgggctaca acatcatctc
     1381 catgccgcat cccgacgaca tcaaggcggc gaaaggcgag cgcgggctcg ccgcggagtg
     1441 accgctggcc acggaaggtt tttgtcaacg agccgattgt agattcgacg cgatttatcg
     1501 cgaggacttc atgatcgttt cgtttgttcc gcccgccatg tcgttcccgc gcaagcggga
     1561 atccaggaag ccgctgcgcc tgaaagctgg ctcagcccga attcccgctc tcgcgggaat
     1621 gatccgttgg ctcttgcgtt ccttggccgt gctctcgtta tgcgttctcg ctgcggcgag
     1681 cgcttcggcc cacgccaaaa tcgagaagaa aagcgagcgc gctttccgcg ccttcatcgc
     1741 aaagctctgg ccggaggccg aggcgcatgg cgtgtcgcgg ggcacgttcg accgcgcctt
     1801 tcgcggcgtg accttcgatc cgaaaatcat cgctctgacc cgacgccagg cggaagtgat
     1861 caagccgatc tggggctaca tcgccagcgc agtatcgccg acgcgcgtcg cgcaaggcga
     1921 ggacaaggcg gaaaactatc gcgcctggct cgcccgggcg cagcagaaat acggcgtcga
     1981 tcccgcgatc attatcggca tttggggcct ggagacggat ttcggcggct ttgccggcaa
     2041 tgacaatgtg atccgttgcc tggcgaccct cgcttttgcg cattatcgcg acgattattt
     2101 caaggacgag ctcatcgccg cgctcgaaat ccttgaagag ggcgatattg cgccgggccg
     2161 aatgaaaggc tcgtgggccg gcgcgatggg tcagacccag ttcatgccgt cgagcttctt
     2221 gaaatacgcc gtcgatttcg aaggccatgg ccgaagggac atctggacaa gcgcgccgga
     2281 cgcgatcggc tcgaccgcca attacctcaa ggagcatggc tggcggcccg gcgagccctg
     2341 ggggttcgaa gtcgcgctgc cgcaagattt cgcgctgaga gccgccgacg ccctggatta
     2401 tgcgccgttc agcgccttcg cgcgccgcgg cgtgacccgc gccgacggcc gccccttgcc
     2461 ggaaacgggc gaagcgcaat tgctcatccc ggccgggctt aaagggccga ttttcttggt
     2521 gacgcccaat ttcaaggtca tcaagagcta taacaattcg acgtcctacg ctctggcggt
     2581 cgccctattg ggcgaccgcg cgatgggcgg ggcggagctt gtcggcgcct ggccggtcaa
     2641 cgaccgccgc ttgacgctgg cgcaggcccg cgacatgcag atcaggctca agaaaatggg
     2701 ctatgatgtc ggcaagatcg acggcaagtt cggcgagacc gggcagacgg cgctcagggc
     2761 ctatcaggaa aggaacgggc tgacgccgga cggctacccg acccttgctt tgcttgagcg
     2821 gatgcggaaa cgcccatgaa ttcagcgtcg taaaggtcgg agagcgtgat cgctttaggc
     2881 cccctgctca gaaaattggc cggctcccgc gccgtcaagg cgatcgccgc cgccgtcttc
     2941 ctctctatgg ccggcggccc ggcggtgatg gccagcagcg acaattcgca gaccttctgg
     3001 atcgagcagc gccaactcaa cgaacagcgc cagaaagcgc gcgagcagag ggcgccggcc
     3061 ccggcgccca aggctcgcca gccgcatgat ttcgtgccga ccgagacgac ccgcgccggc
     3121 ggcgccgata gcggcccggt ccaaccgacc ttcttcgtcg acgtgatcgg cgacagcctc
     3181 gctttgctca ccgccgacgg cctgcgcgaa gccttcgccg acaatccgaa aatcacgatc
     3241 agcagcaagg cgcgcgactc gtcggggttg gtgcgcgacg atttctacga ctgggtcaag
     3301 gcggcgcacg atcttgtcgc ggagcgcagc aacaagaacc ggatcgatct ggcggtgatg
     3361 gagatcggcc tcaacgacat gcaggcgatc aaggagggcg gcgacagttt cgacccgctg
     3421 agcgaccgct ggcgcgacat ttacggcaag cgcgtcgagg cggtcgccga ggccttccgc
     3481 caggcgcata tcccgctttt gtggatcggc ttgccgccga tgaagaacga caagttcaac
     3541 gaccagatga gcaagctcaa cgaactcatc aaggaccacg ccgagaaggc gggcgccaaa
     3601 tatatcgaca tctggaatgc tttcgccgac gaaaacggcg cctatgccgc gtttggcccg
     3661 gacgtgaatg ggcggatcgt caggctgcgc atcgccgacg gcgtctatta caccaaggcc
     3721 ggcgcgcgca aggccgcgca attcgtcgag ggcgacatcc gccgcgcttt cgacgccgcc
     3781 aagccggccg acgacatggt cgatttgccg cccgacgtcg agcaggcgac gatcgacatc
     3841 aacgccgaga ttcggcggga aatgggcctg gcgccgaatt cgagcgaggt cgcgccgccg
     3901 gaagcggata agcccctggc cgggccggtg ctgaatttga cggcaaggcc ggtctcgccg
     3961 ggcggcgcgc ttctctcgcg ctcgccctgg agcaatggcg gcgacgcggc gctcaacacg
     4021 ctggcgagcg gcgtcgagcc gccggccaag gccggccgcg ccgacgactt cgcctggccg
     4081 gccaagccct agcgccgcgg aagaggcggg cgcccgttct tgtcccttag ggagccttgt
     4141 gataggcgac gccccgcaac agaacccgtt tttcgcgtct atggagagat caaatggccg
     4201 accaaaatgg cggcgcggcg ccggaacccg ccaatgctgg ccccagactc tccgttctgg
     4261 tgcaatatac gaaggacttt tccttcgaaa atcccaacgc gccgcgttct ttcgggccgc
     4321 aacagcagca gcccaatatc tcgatccagg tgaacgtcaa cgcccgtcag atcgggccga
     4381 ccgattacga gtcggtgctt gtgctcgaag gcggcgcagg cgagggcgcg aacacgctgt
     4441 tcaagttcga attgaactat gccggcgtgt tccggatcga aaacattccc gccgagcaga
     4501 tccagcacgt cgtgatggtc gaaggaccgc ggctgctctt tcccttcgcc cggcagatca
     4561 tcgccgacgc ggttcgctcg ggcggctatc cgccgctcta tgtcgatccg atcgatttca
     4621 acgcgctgta tttccaacgc atggcggccg ccaatgcgac ggcgcccacg gcaggcgtcg
     4681 cttagcgcgc cttagcctca cccgaccgct tcgcggctcg ccgccgcagg gagaggcggt
     4741 tcggccagat agcgccgcca gatcgcgtgc tcgccgaagc tctcgatata gcgggcgtga
     4801 gcggcgcgct cggcgtcggt gatcagcggg ccgacgattc tcgcgcgatc gatggcgcaa
     4861 ttgaccgccc gggccgcggc ccgggcttcg gcgcgcggcg cggcgaagac gagcgacttc
     4921 tgccgtccgc ccaaaagctc gccatagact tcgaccagaa tctcggcgtc aagcagcgcg
     4981 ccatgcctga cgcggcggct gcgatcgatg tggtagcggt cgcagagcgc gtcgagattg
     5041 ttcggcgcgc cgggatgctt gcgccgcgcg atcgccaatg tatcgacaat gcggctcggc
     5101 gcgatcggct cggcgccgag ccgcgccaat tccgcattga tgaatttgat gtcgaactcg
     5161 gcgttgtgaa tcaccaacgg gtcgtcgccg ataaaggcga gaaaatcggg cgcgacatcc
     5221 gcaaaaagcg gctgccgcga cagcttctcc gtcgaatggc catggacgcg gaaggcgtcg
     5281 tcgggaacgt cgcgctccgg attgatcaat gtatggaaga ccgcgccggt cggaatctga
     5341 tggacgatct cgatgcagcc gatctcgacc atccgatggc cctcggccgg gtcaagaccg
     5401 gtcgtctcgg tgtcaagaac gatttcgcgc atgaagatag aggttccgcc ccgtcatgcc
     5461 ggcaatgctg cgcaacaggc ctcgcatctt cgccctcgtc gcgtcgaagg gacaatcggt
     5521 ttcgatgacg aaatgggcgc ggcggcgctt ctc
//
LOCUS       DATGJW010000179        11453 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_32976, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000179 DATGJW010000000
VERSION     DATGJW010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11453)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11453)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11453
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32976"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..986
                     /locus_tag="VKU03_05340"
     CDS             <1..986
                     /locus_tag="VKU03_05340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HLJ70719.1"
                     /translation="PKMLELSVYDGIPHLLTPVVTDPKKAVVALKWAVREMEGRYKKM
                     SKLGVRNIDGFNARVAEALEKGEAITRIVQTGFDRETGEAIYEQEQMDLTVLPYIVVI
                     VDEMADLMMVAGKDIEGAIQRLAQMARAAGIHLIMATQRPSVDVITGTIKANFPTRIS
                     FQVTSKIDSRTILGEQGAEQLLGQGDMLYMAGGGRISRVHGPFVSDSEVEKIVAHLKT
                     QGQPEYLTAITEEEAPADEDGGGNFAPGTMDAEESSDYYDRAVAIVLRDRKCSTSYIQ
                     RRLSVGYNKAASLVERMEKEGVVGPANHAGKREILVGEGVDRGAFSVEQDD"
     gene            1218..1940
                     /locus_tag="VKU03_05345"
     CDS             1218..1940
                     /locus_tag="VKU03_05345"
                     /inference="COORDINATES: protein motif:HMM:NF015510.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer-membrane lipoprotein carrier protein LolA"
                     /protein_id="HLJ70720.1"
                     /translation="MMRPVKLLKIALPALLAATLSVAQAEDGPVDLHPAKSSKAAPRP
                     PARPAAPGATLAAPMSAAEAIRRANAYLDGAHVMTADFVQIGPDGKRSEGKLFVQRPG
                     RLRFQFAPPDRLDIVADGRSVAVRDQKLGTQDLYFIGQTPLKFLLADHIDISRDTQVQ
                     NVSVTPDAVAITIFDKATFGGSATITLIFDPATFALKQWTVLDSQGLQTVVTLFNVDL
                     ATVPDPALFKIDEQQQMMNSRR"
     gene            complement(2030..2719)
                     /locus_tag="VKU03_05350"
     CDS             complement(2030..2719)
                     /locus_tag="VKU03_05350"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HLJ70721.1"
                     /translation="MLRHTAYVGLIAAALAAPPAFAQTAAGQTTTDKAGSAMQATTPA
                     NPAAAGQPAAPAPKPVATTPASASPTFITQQAKDQWRAGKLVGVAVYGKNQKKVGSVK
                     DILMDHDGNAQAIVISIGGFLGIGSKDVAVPFKAMQWQTEGRAAATNPPPVSTTGTGN
                     GMTPNQGAAQPAPAAQKESPADIEASQGYPDRGVIAMTEAQLKSAPDFHYASENTASG
                     SSAPASTAKKP"
     gene            complement(2792..3109)
                     /locus_tag="VKU03_05355"
     CDS             complement(2792..3109)
                     /locus_tag="VKU03_05355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70722.1"
                     /translation="MRKLVTALLTSAAIGAGVIASTTPAAAFWPLLIPVLIGAGAGGV
                     IAGSAIATAYPPPPMTTTSTGPNSFPPPSTAFGPPAPALEPGCAYSRRLVDGRWRRVE
                     ICD"
     gene            3557..4366
                     /gene="xth"
                     /locus_tag="VKU03_05360"
     CDS             3557..4366
                     /gene="xth"
                     /locus_tag="VKU03_05360"
                     /EC_number="3.1.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008311 - double-stranded DNA 3'-5' DNA
                     exonuclease activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HLJ70723.1"
                     /translation="MRFTIATWNINSVRLRIGLVGRYLKQHAPDILCLQETKCRDAEF
                     PSGEFHRLGYDHIAINGQKGYHGVAIVSKIPIKETEKRGFCNKGDARHMRVTLGGGAT
                     DGLDIHNFYVPAGGDEPDPAINEKFAHKLAFLDELDDWTRSGKIASRRAILVGDLNIA
                     PLEHDVWSHKQLLSVVSHTPIEVEKLNRAQTSGAWVDALRKFVPESEKLYTWWSYRSP
                     DWAKANKGRRLDHIWVSTELGSSVKSMNILRDARGWERASDHVPAMAAIDL"
     gene            complement(4389..4964)
                     /locus_tag="VKU03_05365"
     CDS             complement(4389..4964)
                     /locus_tag="VKU03_05365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein"
                     /protein_id="HLJ70724.1"
                     /translation="MSEQAKKRSSQPWLHQRGVEVQKFGTVRQFPIALSADARLYSCE
                     RLNQILADTQILYALYKKHHWLMRGATFYQLHLLLDKHAEEQNKLIDAIAERVQTLGG
                     VAVGDPRHVAEITRIPRPPNGAEEPPAMLSRLLEAHETILIDAHDAAKRTDEQGDDGT
                     NDLIVSDVIRTGELQAWFLAEHLVDTPLVRA"
     gene            complement(5053..5595)
                     /locus_tag="VKU03_05370"
     CDS             complement(5053..5595)
                     /locus_tag="VKU03_05370"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0022904 - respiratory electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b"
                     /protein_id="HLJ70725.1"
                     /translation="MSAGPVWPLPIRLLHWLGAAAILLMLALGFAMVNLVGDPGARFD
                     LYQAHKSIGIFVLALMLVRIALRFMRAKPAPIASIPPWQNRAAAAMHGLLYALIIALI
                     LAGYAMVSTSPLPLPVALPFGFTAPSLLAPNYALSESFKQLHHVLAALLALCVGGHVA
                     AALKHRFVDRDGTLRRMSLF"
     gene            complement(5592..6197)
                     /locus_tag="VKU03_05375"
     CDS             complement(5592..6197)
                     /locus_tag="VKU03_05375"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HLJ70726.1"
                     /translation="MIRAARRAPIVSCLFSSALAFAALLAASPLRAGPAATPIDPSRT
                     SIQFIVDGIGWPRTKGSFGSFSGRIAVDLRKPETSSVVFRVAAKSIQVGSPSFADYLR
                     GDAFFDVARYPEITFVSTHVERLDDHHARVVGDLTLRGVTRPLTVDVAVEGAAAGEKN
                     RLRFRATGIVHRLEFGMDAGFPAIGNDVDLIIATEAEAGAT"
     gene            complement(6319..7542)
                     /locus_tag="VKU03_05380"
     CDS             complement(6319..7542)
                     /locus_tag="VKU03_05380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1501 domain-containing protein"
                     /protein_id="HLJ70727.1"
                     /translation="MLLCETPSLSRRALLAAGGALFSWAYLPRFARAESARDPRLVVM
                     VLRGALDGLSAVAPIGDPDYPALHGALALRLDGEHAALPLDSFFALNPAMPNFARLYQ
                     ARQALTVHACATNYRERSHFDGQDVLESGMPGPGRVESGWLNRALGLLPKGERVAPRG
                     LAVGSTPPLVLRGGAPVLGWAPSGLPAVSDDLTARLLDLYNHRDPGLAAALKAGLDAD
                     RLAMKQGMEGLKPGGDVAAAMRKAAEGAARLVASDDGPRVAAMAFDGWDTHANEGGAT
                     GRLAQLLRGLDGAIAAFESELGPLWKDTAIVAITEFGRTVHINGTVGTDHGTGTVAFL
                     AGGAVAGGRVVADWPGLKTADLYEGRDLKPTTDLRAVLKGLLADQFGLSARALGETVF
                     PDTAGVAPMKGLIAA"
     gene            complement(7548..9023)
                     /locus_tag="VKU03_05385"
     CDS             complement(7548..9023)
                     /locus_tag="VKU03_05385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014492032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1800 family protein"
                     /protein_id="HLJ70728.1"
                     /translation="MSTDAQRIVVAFNRFGFGPRPGDLAKTSDPHAVLFAELEAPDGA
                     TFVDDSLPRSAAAVQTVFADQERKRLEREQRARLEFEQAGMPAPAVAPLLELKSPPEP
                     SKESQAMMSGGSPPMKPPPSPEQQIFRAEASARLRRAIAAPVGFIERLVAFWSNHFCV
                     SVAKSNIVRATAGAYEREAIRPYVTGRFADMLRAVEQHPAMLNFLDNAQSIGPDSKAG
                     QRRNRGLNENLAREILELHTMGVGSGYTQADVIGLARILTGWTFAGRDGRLGEPGAFV
                     FNANAHEPGPATVLGRTYAQAGLAQGEAALADIARQPAVADHIAKKFARHFVADDPPA
                     ALVGRLAKSFRDSDGDLGVLARTLIADDAAWSAPATKMRNPWELVVASARAFDRPYAE
                     PGPALNAMNLLGMPLWRPGGPNGFAEEASAWASPEGMKTRLDLAAQFARQVRDAPSPT
                     ALIDDILGPSASEATRQAVTRAESREQAYALLLMSPEFQRR"
     gene            9184..9870
                     /locus_tag="VKU03_05390"
     CDS             9184..9870
                     /locus_tag="VKU03_05390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016841446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLJ70729.1"
                     /translation="MAQRRQILIVDDDAELREALAEQLAMHEEFEISAAENAANARQM
                     IQASPPDLVVMDVGLPDMDGREAVRLMRKDGFKRPIIMLTAHDSDADTVLGLDAGAND
                     YIAKPFRFAVLLARIRVQLRQHEASEDAEFQIGPYTFRPASKNLISAKGNRLRLTEKE
                     AAILRYLRRADRQVVSRETLLKEVWGYNSNVTTHTLETHIYRLRQKIEADPADARLLV
                     TDAGGYKLLP"
     gene            9966..10418
                     /locus_tag="VKU03_05395"
     CDS             9966..10418
                     /locus_tag="VKU03_05395"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLJ70730.1"
                     /translation="MALEQDIGRLARTRPFDVLPRDALKLIAFSAERRTFPAGRTLFE
                     QNDEADCAYFLLSGSVALTAYGKDEPQERVVGAGALIGEMAMFAPLARPASARTKEET
                     AALRIPRHLMARVLSEYPREAAQIRAQLAERTRQITAEFEAVRQRARL"
     gene            10601..>11453
                     /locus_tag="VKU03_05400"
     CDS             10601..>11453
                     /locus_tag="VKU03_05400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="porin"
                     /protein_id="HLJ70731.1"
                     /translation="MTILQRIFLAGAAMLLALEGAKAADLPTSKPAPPPLLGPTSCAD
                     PVQFVTTDCALSWYGVTVYGTVDMGLGWESHGVPLNKWIISGVEELVQKNSNRPLWLL
                     TPGGLSQSFIGVKGKEEFAPGWSFVFDLNMGFDPYTLTVANGPRSYLENNGLPLALQS
                     ANADSSRAGQFYNGVGYAGISSKAFGTLTVGRVNSLTLDGVVAYDPMGASYAFSVIGW
                     SGLTAGVGDTEDARATTAVKYRVDLGAFRASALYQFGGYAANNGAQNAFQVGFGGDFG
                     GGGSGKLS"
BASE COUNT         2059 a         3707 c         3816 g         1871 t
ORIGIN      
        1 atccgaaaat gctcgagctg tcggtttatg acggcattcc gcatctcctg acgccggtcg
       61 tgacggatcc gaagaaggcg gtggtcgcgc tcaaatgggc agtgcgcgaa atggagggcc
      121 gctacaagaa aatgtcgaag ctcggcgtgc gcaacatcga cgggttcaac gcccgcgtcg
      181 ccgaagcgct ggagaaaggc gaggcgatca cccgcatcgt ccagaccggc ttcgatcgcg
      241 agaccgggga ggcgatctac gagcaggagc agatggacct tacggtcctg ccctatatcg
      301 tcgtgatcgt cgacgaaatg gccgacctga tgatggtcgc cggcaaggac atcgaagggg
      361 cgatccagcg cctcgcccag atggccagag ccgccggcat ccatctgatc atggcgacgc
      421 agcgcccctc ggtcgacgtc atcaccggca cgatcaaggc gaactttccg acccgcatct
      481 ccttccaggt cacatccaag atcgacagcc gcaccattct cggcgagcaa ggggccgagc
      541 aactgctcgg ccagggcgac atgctctaca tggccggcgg cggccgcatc tcgcgcgtgc
      601 atgggccgtt cgtctctgac agcgaggtcg agaaaatcgt cgcccatctc aagacgcaag
      661 ggcagccgga atatctgacc gcgatcaccg aggaggaagc gccggcggac gaggatggcg
      721 gcggcaattt cgcgcctggc acgatggacg ccgaggaatc ctccgattat tacgaccgcg
      781 ccgtcgctat cgtgctgcgc gaccgcaaat gctcgacgag ctacatccag cgtcggctgt
      841 cggtcggcta caacaaggcg gcgtcgctgg tcgaacggat ggagaaggag ggcgtggtcg
      901 gtcccgccaa tcatgccggc aagcgggaaa ttctggtcgg cgaaggcgtc gatcgcggcg
      961 ccttttcggt cgagcaggac gattaggcgg cgcggcgtcc caatcccgcc acatcgagcg
     1021 cttagagcgt cgaaaagtag cgttgcggcg ttccggcttg cgttcgtaag agcgtccctt
     1081 ccggccgggc tcgacggcgg cgcgttattc ggcctagcgt taaaaaggcc aagcggcgtc
     1141 gagcccacgg ggtcaagcgc aatggttgtc ggcgaacaag cggtcgcgcg gagcgagccg
     1201 ggagagaagt tgcgcggatg atgcgcccag tgaaactgct gaaaatcgcc ctgcccgcgc
     1261 tgctggccgc aacgttgagc gtcgcccagg cggaggacgg accggtcgat ctgcatcctg
     1321 ccaaatcgtc caaggcggcg ccgcgcccgc cggcccggcc tgcggcgcca ggcgcgacgc
     1381 tcgccgcgcc gatgagcgcc gccgaggcga tccgccgggc caacgcctat ctcgacggcg
     1441 cgcatgtgat gaccgccgat ttcgttcaga tcgggcccga cggcaagcgc tcggaaggca
     1501 agctttttgt gcagcggccg ggccggctgc gcttccaatt cgcgccgccc gaccggctcg
     1561 acatcgtcgc cgacggccgc agcgtcgcgg tgcgcgatca gaagctcggc acccaagacc
     1621 tctatttcat cggccagact ccgctgaaat ttctgctcgc cgatcacatc gacatttccc
     1681 gcgacacgca ggtccaaaac gtctcggtca cgccggacgc ggtcgccatc accattttcg
     1741 ataaggcgac attcggcggc tcggcgacga tcacgctgat cttcgatccc gccactttcg
     1801 cgctcaagca atggaccgta ctcgattcgc aggggctgca aaccgtggtc acgctgttca
     1861 acgtcgatct cgcgaccgtg cctgaccccg ccctgttcaa aatcgacgaa cagcagcaga
     1921 tgatgaattc gcggcgctga atgcgacaag gcccccagcg ctttcacgct ggaggcctct
     1981 cgacggtcct cggcgaggga ggggcgccga ggcgtcttac ctcgtcacgt cagggcttct
     2041 tcgccgtgga ggccggcgcg ctcgagccgc tggccgtatt ctccgacgca tagtggaaat
     2101 ccggcgcgct cttgagctgc gcctcggtca tcgcgatgac gccgcgatcc ggatagccct
     2161 ggcttgcttc gatatcggcc gggctttcct tttgagcggc cggggcgggc tgggccgcgc
     2221 cctgattcgg cgtcatgccg ttgccggttc cggtggtcga aacgggcggc gggttggtgg
     2281 ccgcggcgcg tccctcggtc tgccattgca tcgccttgaa cggcaccgcg acatccttcg
     2341 agccgatgcc gaggaagccg ccgatgctga tgacgatcgc ctgggcgttg ccgtcatggt
     2401 ccatcaggat atctttgacc gagccaactt tcttttgatt cttgccgtag accgcgacgc
     2461 cgacgagctt gccggcgcgc cattggtctt tggcttgttg cgtgatgaag gtcgggctgg
     2521 cgcttgccgg cgtcgtcgcg accggcttcg gcgcaggcgc ggccggttgg ccggcggcgg
     2581 ccggattggc tggcgtcgtc gcttgcatcg cggagcccgc tttgtccgtc gttgtctggc
     2641 ccgcggccgt ctgagcgaaa gcgggaggcg ccgccagcgc cgcggcgatc aatcccacat
     2701 aggcggtgtg tctcagcatg ttcgtctctc catctttaag gaaaacgccc cagccgaaaa
     2761 cgacgcccgc cggaacggcg ggcggcgccg atcagtcgca gatctcaacg cgcctccacc
     2821 ggccgtccac gaggcggcgg gaataggcgc agccgggctc gagggccggc gcaggcggtc
     2881 cgaaagccgt cgacggcggc gggaacgaat tgggtccggt cgacgtggtc gtcatgggcg
     2941 gcggcggata ggccgtcgca atcgccgagc cggcgatcac gccgcccgct ccagcgccga
     3001 tcagcaccgg aatcaggagc ggccagaacg ccgccgccgg cgtcgtcgag gcgatcacgc
     3061 cggccccgat cgccgcgctc gtcagaagcg ccgtcactaa ttttctcatc ctctggtctc
     3121 cgatttgtcg tcgctatgca gagcgcgcca cagcggcgag gaaagttacg tctcagaacg
     3181 cggttttgtt cccctcgcct cgatatcggc caagttcagc cgtgaataaa aaaagcatag
     3241 aggcgcgccc agccgagagg cggcgggcaa tttggccatt gaggcgcaaa gccgatcaaa
     3301 gttgtggccg cattgtgatg gaattcatat tccggttatg aagaatatta ctgtcgaaat
     3361 cggaagccgc cgcaattcgc atccgagaaa aggcgaaaca cgaccggaaa catctccagc
     3421 cgaagcgtcg catctttggg cattttgaaa aatcgccgcg gcgccgaagg ggcctcgctt
     3481 gtgcgtcatc ggccaagccc cttgccaaag gcgccggaag cctgatttgc agggagcttc
     3541 ccatgcggag ctcgccttgc gcttcacaat cgccacctgg aacatcaact cggttcgcct
     3601 gcgcatcggc ctggtcggcc gctatctcaa gcagcacgcc ccggacattc tttgcctgca
     3661 ggagaccaag tgccgggacg cggagtttcc gagcggcgag ttccatcgcc tcggctacga
     3721 ccacatcgcg atcaacggcc agaagggcta tcacggcgtc gccatcgtct cgaaaattcc
     3781 gatcaaggag acggagaagc gcggcttctg caataaaggc gacgcgcgcc acatgcgcgt
     3841 taccctcggc ggcggcgcga ccgacgggct cgacatccat aatttttatg tgccggccgg
     3901 cggcgacgag cccgatcccg ccatcaacga gaaattcgcc cacaagctcg cctttcttga
     3961 cgagctcgac gactggacgc gaagcggcaa aatcgcgtct cgccgcgcga tcctcgtcgg
     4021 cgatctgaac attgcgccgc tcgaacacga tgtctggagc cacaagcagc tcctctcggt
     4081 cgtcagccac acgccgatcg aagtcgagaa gctcaaccgg gcgcagacca gcggcgcgtg
     4141 ggtggacgcg ctgcgcaagt tcgtccccga gagcgaaaag ctttacacat ggtggagcta
     4201 tcgctcgccc gattgggcca aggccaacaa gggacgccgg cttgaccata tttgggtcag
     4261 cacggagctc ggctcgtcgg tcaagagcat gaacattctg cgcgacgcgc gcggctggga
     4321 gcgcgcctcc gaccatgtcc cggcgatggc cgcgatcgac ctttagtacg tcgtcattgc
     4381 gagcggagct aagctcgcac aagcggcgta tcgaccagat gctcggcgag gaaccaggcc
     4441 tgcaattcgc cggtgcgaat cacgtcggat acgatcagat cgttggtgcc gtcgtcgccc
     4501 tgttcgtcgg tcctcttcgc cgcatcgtgg gcgtcgatca ggatcgtctc gtgcgcttcg
     4561 agcagccgcg acagcatcgc cggcggctcc tcggcgccgt tgggcgggcg cggaatgcgg
     4621 gtgatctcgg cgacgtggcg gggatcgccg accgcgacgc cgcccaacgt ctggacgcgc
     4681 tcggcgatcg cgtcgatcag cttgttctgc tcctcggcat gtttatcgag caaaagatgc
     4741 aactggtaga acgtggcgcc gcgcatcagc cagtgatgct tcttgtagag cgcatagagg
     4801 atttgcgtat cggcaaggat ctggttgagc ctttcgcacg agtagagcct ggcgtcggcc
     4861 gaaagggcga tcggaaattg ccgcaccgtg ccgaactttt gcacttcgac gccgcgctgg
     4921 tgcagccagg gctggctcga acgctttttc gcttgttcgc tcaatgaggc ctccttcgga
     4981 cgccggttgt cgggcaaacg ggcgttcgcc cacatggcgc gcgccgaccg ccgagttcac
     5041 gcggctaaag gcctagaaca agctcatgcg gcgcaaagtt ccatcgcggt cgacgaagcg
     5101 atgcttgagc gccgccgcga catggccgcc gacgcagagc gccagaagcg cggccagtac
     5161 atgatgcaat tgcttgaagc tctccgacaa ggcgtagttg ggcgcgagca gactaggggc
     5221 cgtgaagccg aagggcagcg caaccggcag aggcagcggc gaggtcgaaa ccatcgcgta
     5281 gccggcaagg atcagcgcaa tgatcagagc atagaggaga ccatgcatcg ccgcggccgc
     5341 gcggttttgc caaggcggaa tgctcgcgat cggcgccggc ttggcgcgca tgaagcgcag
     5401 ggcgatccgc acaagcatca aagccaaaac gaaaatcccg atcgatttgt gcgcctgata
     5461 gagatcgaac cgcgcgcccg gatcgccgac caggttgacc atggcgaagc cgagcgccag
     5521 catgagcaga atcgccgccg cgccgagcca atggaggaga cgaattggca gcggccagac
     5581 cgggccggcg ctcatgtcgc gccggcttcc gcttccgtcg cgatgatcaa atcgacatcg
     5641 ttgccgatcg ccggaaaacc ggcgtccatg ccgaactcca gacgatggac gattccggtc
     5701 gcgcgaaagc gcaagcgatt tttttcgccc gcagccgcgc cttcgaccgc gacgtcgacg
     5761 gtgagcggcc tggtcacgcc gcgcaaggtc agatcgccga cgacccgcgc atgatgatcg
     5821 tcgagcctct cgacatgagt cgagacgaag gtgatctccg gatagcgcgc cacgtcgaaa
     5881 aaagcgtcgc cgcgaagata gtcggcgaag gacggcgagc ccacctgaat ggacttggcg
     5941 gcgacgcgga acaccacgct gctcgtttcg ggcttgcgca aatcgacggc gatccggccg
     6001 gaaaagctgc cgaaactgcc cttggtgcgc ggccagccga tgccgtcgac gatgaattgg
     6061 atgctcgtgc ggctgggatc gatcggcgtc gccgccggcc cggccctcag cggcgaggcg
     6121 gcgagaagag cggcgaacgc cagagcggat gaaaacaagc acgaaacgat cggcgcgcga
     6181 cgcgcggcgc gaatcatggc gcccctttct gcgattatga acgccggcgg cgcgacgggc
     6241 cggcggctcg cagtgaacag tcggagcgac gagattattc gctttcggca gagcggcgcc
     6301 gacggaccgg cgccgctgct atgcggcgat gaggcctttc atcggcgcga cgcccgccgt
     6361 gtccgggaac accgtctcgc cgagcgcgcg cgccgacagg ccgaactggt cggcgagcaa
     6421 gcccttgagc acggcgcgca gatcggtcgt cggcttcaga tcgcggccct cgtagagatc
     6481 ggccgtcttc aggccgggcc agtcggcgac gacccggccg ccggcgacgg cgccgccggc
     6541 taagaacgct acggtccccg tcccgtgatc ggtgccgacc gtgccgttga tatgaacggt
     6601 gcggccgaat tcggtgatcg cgacgatcgc cgtatccttc cacagcgggc cgagctcgct
     6661 ctcgaacgcc gcgatcgcgc cgtcgaggcc gcgaagaagc tgcgccaggc ggccggtcgc
     6721 gccgccttca ttggcgtgcg tatcccagcc gtcgaaggcc atcgccgcga cgcgcgggcc
     6781 gtcgtcggag gcgaccagcc gcgccgcgcc ctccgccgct ttgcgcatcg ccgccgcgac
     6841 gtcgccgccc ggcttcaacc cctccatgcc ctgtttcatg gcgagccggt cggcgtcgag
     6901 gccggctttg agcgccgcgg ccaagcccgg atcgcgatga ttgtaaaggt cgaggagcct
     6961 ggcggtgaga tcgtcgctta ccgccggcag gccggacggc gcccagccga gcaccggcgc
     7021 gccgccgcgc agcacgagcg gcggcgtcga accgaccgcg agcccacgcg gcgcgacgcg
     7081 ctcgcctttc ggcagcaggc cgagcgcgcg attgagccaa ccgctttcga cgcggcccgg
     7141 cccgggcatg ccgctttcga gcacgtcctg tccgtcgaaa tgcgagcgct cgcggtaatt
     7201 ggtggcgcag gcatgcacgg tcagcgcctg gcgcgcctga tagagcctgg cgaaattcgg
     7261 catggcggga ttgagcgcga agaacgaatc gagcggcaga gccgcatgct cgccgtcgag
     7321 tcgcaaggcg agcgcgccat gcagcgccgg ataatcggga tcgccgatcg gcgccaccgc
     7381 cgagagcccg tccagcgcgc cacgcagcac catcacgacg aggcgcggat cgcgcgcgct
     7441 ttcggcccgc gcgaagcgcg gcagataggc ccaggagaag agcgcgccgc cggcggcgag
     7501 caaggcgcgg cgcgacaggg acggcgtttc acagagcagc atcggattca ccggcgttgg
     7561 aattcgggag acatcagcag cagggcgtag gcctgttcac gcgattcggc gcgcgtcacc
     7621 gcttgccgcg tcgcttcgga ggcgctcggc ccgagaatgt cgtcgatcaa ggcggtcggg
     7681 ctcggcgcat ccctgacctg gcgagcgaat tgcgcggcaa gatcgaggcg cgtcttcatg
     7741 ccttccggcg aggcccaggc gctcgcctcc tcggcgaagc cgttgggacc gccgggccgc
     7801 cagagcggca tgccgaggag attcatcgcg ttgagcgccg ggccgggctc ggcataggga
     7861 cgatcgaagg cgcgagcgga ggcgacgacg agctcccagg gattgcgcat cttggtcgcc
     7921 ggcgcgctcc aggcggcgtc gtccgcgatc aaggtccggg cgagaacgcc gagatcgccg
     7981 tcgctgtcgc ggaaggattt ggcgagccgg ccgaccaagg cggccggcgg atcgtcggcg
     8041 acgaaatggc gcgcgaattt cttggcgatg tgatcggcga ccgccggctg gcgggcgatg
     8101 tcggcaagcg cggcttcgcc ctgggcgagg ccggcttgcg cataggttcg gccgagcacg
     8161 gtcgccgggc caggctcatg cgcattggcg ttgaagacga acgcgccggg ctcgccgagc
     8221 cttccgtcgc gcccggcgaa ggtccagccg gtgagaatgc gcgccaagcc gatcacatcc
     8281 gcctgggtgt agcccgagcc gacgcccatc gtgtgcaatt cgaggatttc gcgcgcgaga
     8341 ttctcgttga ggccgcggtt gcgcctctgg ccggccttgg aatccggccc gatcgactgc
     8401 gcattgtcga ggaagttgag catcgccgga tgctgctcga cggcgcgcag catgtcggcg
     8461 aagcggccgg tgacataggg cctgatcgcc tcgcgctcgt aagcgccggc cgtcgcccgc
     8521 acgatgttgc tcttggcgac cgagacgcag aaatgattcg accagaacgc gacgagccgc
     8581 tcgataaagc cgactggcgc ggcgatcgcc cgccgcagcc tcgccgatgc ttcggcgcga
     8641 aagatttgct gttcgggcga aggcggcggc ttcatcggcg ggctgccgcc gctcatcatc
     8701 gcctggctct ctttggacgg ctcaggcggg gatttcagct cgaggagcgg cgcgacagcg
     8761 ggcgccggca tgcccgcctg ctcgaattcc aggcgggcgc gttgctcccg ttcgagccgc
     8821 ttgcgctcct gatcggcaaa gacggtctgc accgccgccg cgctgcgcgg cagcgaatcg
     8881 tcaacgaagg tcgcgccgtc cggcgcctcc agttcggcga agagaaccgc atgcggatcg
     8941 ctcgtcttgg cgagatcgcc tggcctcggc ccgaagccga atcggttaaa ggcgacaaca
     9001 atgcgctgag cgtccgtcga catgaccgtt cattgtcttg cgcccccgcg acgcccaaga
     9061 aagcatagcc aacatgacca gaacgtgaac agcgcgcctc ttgcgcgcgt tatcgtgctt
     9121 ggctagccgg accgcccgtg gccggactat gatcggccgc gggggcgatt gcggaaagaa
     9181 agtatggctc aaaggcggca aattctgatc gtcgacgacg acgccgagct tcgcgaggcg
     9241 ctcgccgagc aattggcgat gcacgaggag ttcgaaatct ccgcggccga aaacgcggcc
     9301 aatgcgcgcc agatgattca agcaagcccg cccgacctgg tggtcatgga tgtgggcttg
     9361 cccgacatgg acgggcgcga agccgtgcgg ctgatgcgca aggacggctt caagcggccg
     9421 atcatcatgt tgacggcgca tgattcggac gccgacaccg tgctcggcct cgacgccggg
     9481 gccaacgatt atatcgccaa gccgttccgc ttcgcggtgc tgctcgcccg catccgggtg
     9541 caattgcgcc agcacgaggc gagcgaggac gccgaattcc agatcgggcc gtatacgttc
     9601 cggccggcct ccaaaaacct catcagcgcc aagggcaatc gtctgcgcct gaccgagaag
     9661 gaggcggcga tcctgcgcta tttgcggcgc gccgaccggc aggtggtctc gcgcgagacc
     9721 ctgctcaagg aagtttgggg ctacaattcc aacgtgacca cccacacgct tgagactcac
     9781 atctacaggc tgcggcaaaa aatcgaagcc gatccggccg acgcgcgctt gctggtcacc
     9841 gacgccggcg gatacaagct gctgccgtga tgggtcgacg cgccagagaa agctgcggtt
     9901 gaaagccgac ccggtctggc ttcccgctta cgcgggaatg acatgaaagg aataggggag
     9961 cgaccatggc gcttgaacag gatatcggcc ggctggcgcg aacgcggccg ttcgacgtcc
    10021 tgccccgcga cgcgctgaag ctcatcgcct tttccgccga gcgccggacc ttcccggctg
    10081 gccgaacgct tttcgaacag aatgacgagg ccgattgcgc ctatttcctg ctctcaggat
    10141 cggtggcgct gaccgcctac ggcaaggacg agccgcaaga gcgtgtcgtc ggggccggcg
    10201 cgttgatcgg cgaaatggcg atgttcgcgc cgctggcgcg gccggctagc gcgcggacaa
    10261 aagaagaaac ggcggcgctg cgcatcccgc gccatctcat ggcgcgcgtt ctaagcgaat
    10321 atccgcgcga agcggcgcaa atccgcgccc agttggccga gcgcacgcgc cagatcactg
    10381 cggaattcga ggcggtgcgc cagcgcgcgc gcctgtgagc ggctgccgct ccacccgcca
    10441 ataatcgatc aggaacagcg tctcgcccgc cgggaccgag ttggctccgc cggtacattg
    10501 accgctccgg tcgttatatt ttattgaata taacgcttgg cggctcgccg agcgggacga
    10561 tcgcgcttgc cgatttcagg cgcgcaatga gggggctctc gtgacaattc tgcaaaggat
    10621 atttcttgcc ggcgcggcca tgcttctcgc cttggagggc gcgaaggccg ccgatttgcc
    10681 gaccagcaag ccggcgccgc cgccccttct cggcccgacc tcctgcgccg accccgtcca
    10741 gttcgtgacg accgattgcg cgttgagctg gtacggcgtc accgtctacg ggacggtgga
    10801 tatgggcctc ggttgggaga gccacggcgt gccgctcaac aaatggatca tttccggggt
    10861 cgaagaactc gtccagaaaa acagcaaccg gcctttatgg cttctgaccc ccggcggact
    10921 cagccagtcg tttatcgggg tcaaaggaaa agaggagttt gcgcctggct ggtcgttcgt
    10981 cttcgacctc aatatgggtt tcgatcctta cacgctcacc gtcgccaatg gaccgcggtc
    11041 ctaccttgag aacaacggcc tgcctttggc gctgcagagc gccaacgccg actccagtcg
    11101 ggcgggacaa ttctataacg gcgtcggcta tgccgggatt agcagcaagg cgttcggcac
    11161 gctgacggtg ggccgggtca attcgctgac attggacggc gtcgtcgcct acgatccaat
    11221 gggggcgtcc tatgctttct ccgtgatagg ctggtccggc cttacggccg gcgtcggcga
    11281 cactgaggac gcgcgcgcga caacggctgt caaatatcga gtcgatctcg gggcattccg
    11341 cgcctccgcg ctctaccagt tcggcggcta tgcggccaat aacggcgctc agaacgcctt
    11401 ccaggtggga ttcggcggcg acttcggcgg gggcggctcc ggcaaacttt cgc
//
LOCUS       DATGJW010000180         6358 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_655911, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000180 DATGJW010000000
VERSION     DATGJW010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6358)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6358)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6358
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_655911"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..980
                     /locus_tag="VKU03_05405"
     CDS             <1..980
                     /locus_tag="VKU03_05405"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ70732.1"
                     /translation="MALNVRRLARLIREEGVDIVHARSRAPAWVALAAMRLQPRPFVT
                     TFHGAYSGRSALKLSYNSVMARGDAVIANSHYTAEMIARLYPFARDKITVIPRGADFA
                     KFSPASVDPARVARLRRAWGVEPQERIVLLAARLTAWKGQKVLIEAARRLKEGGLSGV
                     RYILAGDAQGRSAYVKELDALIEKAGLKGVVARVGHCDDMPAAFLAASAVAVPSTEPE
                     AFGRSAVEAQAMGAPVVVSELGAVPETVLAPPQVAAEERTGWRMPAGDAQKLAEALGH
                     ALSLGASARETLARRARAHVERHFSLEQMTRATLSVYLNALEGRPKSLP"
     gene            complement(943..1875)
                     /locus_tag="VKU03_05410"
     CDS             complement(943..1875)
                     /locus_tag="VKU03_05410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HLJ70733.1"
                     /translation="MSIRPIKRIAQSTPTIEGAGVKLRRAFGFGDPSEFDPFLMMDDF
                     RGDKPSDYAAGFPWHPHRGIETITYVLAGTVDHGDSLGNRGSLGAGDVQWMTAGSGIL
                     HQEMPKGDAKGRMHGFQLWANLPSSLKMTSPRYQDIPAPAIPEVIDDDGTIVRVVCGD
                     FWGKKGPVDGIAAEPRYLDVWVPPGKRKTLPVETYRQAFAYIFEGSGVFSDASQPFGV
                     LHEKQVDGEEILVRETIGNRSLVVFGGGDEVTVQAGEEGVRFLLVSGRPIKEPVAWYG
                     PIVMNTQAEIRQAIAELNSGAFIKEGSSDVLRAR"
     gene            2224..3189
                     /locus_tag="VKU03_05415"
     CDS             2224..3189
                     /locus_tag="VKU03_05415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70734.1"
                     /translation="MLLTASPPKADASTQPVVRVANLSKVYDTGFKALDAVNLEIRRG
                     EIFALLGPNGAGKTTLISIICGIVNASGGSVTADGHDIRTDYRAARAKIGLVPQELTT
                     DMFETVWNTVRFSRGLFGRPANPAHLEHVLREVALWDRRGAKIMTLSGGMKRRVMIAK
                     ALSHEPTILFLDEPTAGVDVELRRDMWRMVRALRDSGVTIILTTHYIEEAEELADRIG
                     VINNGKLVLVEEKAALMHKLGKKTLVVTLHQALSRLPESLAGYGLDLSADGRELTYVY
                     DTARDSVGVRPVLQAIEAAGLAYRDIDTKTSSLEEIFVDIIREQS"
     gene            3186..3947
                     /locus_tag="VKU03_05420"
     CDS             3186..3947
                     /locus_tag="VKU03_05420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70735.1"
                     /translation="MNVHAIEAIYRYEMARTRRTLLQSVIAPVVSTSLYFIVFGAAIG
                     ARMTTIDGVSYGAFIVPGLVMLSLLTQSITNASFGIYFPRFVGTIYEVLSAPISGIEV
                     VIGYVGAAASKSILLGLIILATATLFVPLRIEHPFWMALFLILTAATFSLFGFVIGIW
                     ADNFEKLQVVPLIIITPLTFLGGSFYSIDMLPPLWRKIALVNPVVYLISGFRWSFYGK
                     ADVDIRISLGMTLFFLALCLAAVVAIFRTGYRLKK"
     gene            complement(4044..4238)
                     /locus_tag="VKU03_05425"
     CDS             complement(4044..4238)
                     /locus_tag="VKU03_05425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018640760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HLJ70736.1"
                     /translation="MDREHVKGAADKAKGAIKDAAGKLMDDKKLQAEGKIDKAKGAAH
                     NVAGDAKDAARQAKEDIERR"
     gene            complement(4389..5900)
                     /locus_tag="VKU03_05430"
     CDS             complement(4389..5900)
                     /locus_tag="VKU03_05430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLJ70737.1"
                     /translation="MRPPFLAKSSASPPARAGFACDGGGAVSRSSACECCGPAARALN
                     RRITADLSRRAMLGGAAALLAPFIGLRPAKALSEPPPAPGRPILFSNLLLFDGLDGRL
                     RQGVGVLARGAKFAGLPPAGESVEGAQVIDCGGRLAMPGLIDAHWHAMLCGIRYAEAL
                     TADVPYLHLVAASEAERTLLRGFTTVRDAGGPTFALKRAIDSGLIAGPRIFPSGAMVS
                     QTAGHGDFRQRYEIPRGPQTPLSLTETAGVGVIADGEAEVLRRVREQLLLGATQIKMM
                     AGGGVSSHYDPIDVTQYSESELRAGVGAAEDWGTYVMTHVYTPRGIQRAIRAGVKSIE
                     HGQLTDEESVRMMAGEGVWWSLQPFFSDEDSNPKVDPASRAKAEIVSQGTARAYEFSK
                     QYKIKTAWGTDILFAPQNLPFHARHLAKLTPRFYEPLELLGIATARNGELLRLSGQRY
                     PYDGDLGVIAPGAMADLLIVDGDPTKSLDFLVAPNETLRIVMKDGRIYKNTLG"
     gene            complement(5924..>6358)
                     /locus_tag="VKU03_05435"
     CDS             complement(5924..>6358)
                     /locus_tag="VKU03_05435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911702.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HNH endonuclease"
                     /protein_id="HLJ70738.1"
                     /translation="DRVNIVSQYERTVRSPSFEMRLPSVVSLKTYVKPSRYPAFTRFN
                     VFLRDRFTCQYCGDDDELTFDHVVPRSKGGLTTWENVVAACSRCNLRKGDMLPAAARM
                     WPAKAPFQPTIHDLHQNGRLFPPNYLHESWMDYLYWDSELEP"
BASE COUNT         1051 a         2067 c         2053 g         1187 t
ORIGIN      
        1 ccatggcgct caatgtgcgc cggctggcgc ggctcatccg cgaggaaggc gtcgatatcg
       61 tccatgcccg ctcgcgcgcg ccggcttggg tggcgctggc ggcgatgcgg cttcagccgc
      121 gtcctttcgt gaccacgttc cacggcgcct attcgggacg ttcggcgctg aagctcagct
      181 acaattcagt gatggcgcgc ggcgatgcgg tgatcgccaa ttcgcactat acggcggaga
      241 tgatcgcccg cttgtaccca tttgcccgcg acaagatcac ggtcatcccg cgcggcgcgg
      301 attttgcgaa attctcgccc gcttcggtcg acccggcgcg cgtcgcccgg ctgcgccgcg
      361 cctggggcgt cgagccgcaa gagcgcatcg tgctgctcgc cgcccgcttg accgcctgga
      421 aagggcagaa agtgctgatc gaagccgcca ggcgcttgaa ggaaggcggc ctgagcggcg
      481 tccgctacat cctggcgggc gacgcgcaag gccgcagcgc ctatgtcaag gagctcgacg
      541 cgctgatcga gaaagcgggc ctgaaaggcg tcgtcgcgcg ggtcggccat tgcgacgaca
      601 tgccggccgc gttcctcgcc gcctccgccg tcgccgtgcc ctcgaccgag ccggaagcct
      661 tcggccgcag cgcggtcgag gcgcaagcga tgggcgcgcc ggtcgtcgtc tccgaactcg
      721 gcgcggtgcc ggagacggtg ctggcgccgc cgcaagtcgc agccgaggag cgcaccggtt
      781 ggcgcatgcc ggccggcgac gcgcaaaagc tggccgaggc gctcggccat gctttgagcc
      841 tcggcgcctc ggcgcgcgaa acgttggctc ggcgcgcccg cgcccatgtc gagcgccatt
      901 tttcgctcga acagatgacg cgggcgacgc tgagcgtcta tctcaacgcg ctcgaaggac
      961 gtccgaagag ccttccttga tgaaggcgcc ggaattgagc tcggcgatcg cctggcgaat
     1021 ttcggcttgc gtgttcatca cgatcggccc gtaccacgcc accggctcct tgatcggccg
     1081 gccggagacg agcaggaagc gaacgccctc ctcgccggct tgcaccgtca cttcgtcgcc
     1141 gccgccgaac acgaccagcg agcgattgcc gatcgtctcg cgaacgagga tctcttcgcc
     1201 gtcgacctgc ttctcgtgca gcacgccgaa cggctgcgag gcgtcggaga acacgcccga
     1261 gccttcgaaa atataggcga aggcctggcg ataggtctcg accggcaggg tcttgcgctt
     1321 gcccggcggg acccagacgt cgagatagcg cggctcggcc gcgatgccgt cgaccgggcc
     1381 tttcttgccc cagaagtcgc cgcagacgac gcgcacgatc gtcccgtcgt cgtcgatcac
     1441 ttccgggatc gccggcgccg ggatgtcctg gtagcgcggc gaggtcattt tcagcgacga
     1501 gggcaaattg gcccagagct ggaagccgtg catgcggcct ttggcgtctc ccttcggcat
     1561 ttcctggtgc aggatgccgc tgccggcggt catccattgc acgtcgccgg cgccgaggct
     1621 gccgcgattg ccgaggctgt cgccatggtc gaccgtaccg gccagcacgt aagtgatggt
     1681 ctcgatgccg cgatgcggat gccagggaaa gccggcggca tagtcgctcg gcttgtcgcc
     1741 gcggaaatcg tccatcatca ggaacggatc gaattccgag ggatcgccga agccgaaggc
     1801 gcggcgcagc ttgacgccgg ctccttcgat cgtcggcgtc gattgcgcga tgcgcttgat
     1861 cggacgaatg gacatgggaa gggttccttt tgggggcgcg aagcgccctt tgtcacgccg
     1921 taatgtgggc gccggtcagg caagcgtcca ggggttgggc ttgcgcctat gcaaagggct
     1981 atgggcgcgc ccggctctga agacccagga gcaattgctc tcgggttctt ccaatcacga
     2041 aatgatccgc agcgacgaaa cgcgcgaaaa gctgcgatca tgggtcacca ctagccgccg
     2101 gccaatgcgg aatttgcgcc gctccgatct ttgtctttgt cttcgccaag ctcgcgctcc
     2161 cgccggggcg ggtatatggt gcgcgccctt tcaaccgaaa cctgccgtta aaagttcgcc
     2221 cacgtgcttc tcacagcctc tccgccgaaa gccgacgcgt cgacgcaacc ggtcgtccgt
     2281 gtcgccaatc tctcgaaagt ctacgatacg ggcttcaagg cgctcgacgc cgtcaatctc
     2341 gaaatccgtc gcggcgagat tttcgcgctt cttgggccga acggggccgg caagacgacg
     2401 ctgatctcga tcatctgcgg cattgtcaac gccagcgggg gctcggtgac ggccgatggg
     2461 cacgatatca gaaccgacta tcgcgccgcg cgcgccaaga tcggcctggt gccgcaggag
     2521 ttgaccaccg atatgttcga gacggtctgg aatacggtgc gattcagccg cggcctgttc
     2581 ggccggccgg ccaatcccgc ccatctcgaa catgtgctgc gcgaggtcgc gctgtgggat
     2641 cgtcgcggcg ccaagatcat gaccttgagc ggcggcatga agcgccgcgt gatgatcgcc
     2701 aaggcgctgt cgcacgagcc gaccatcctg tttctcgacg agccgaccgc cggcgtcgac
     2761 gtcgaattgc gccgcgacat gtggcgcatg gtgcgcgcct tgcgcgattc cggcgtcacc
     2821 atcatcctga ccacgcatta tatcgaggag gccgaggagc tcgccgaccg tatcggcgtc
     2881 atcaataacg gcaagctcgt gcttgtcgaa gaaaaagccg ccttgatgca taagctcggc
     2941 aagaagacgc tcgtcgtcac gctccaccag gctttatccc gccttcccga atccctcgcc
     3001 ggctatggtt tggatctgtc ggcggacgga cgcgagctga cctatgtcta tgatacggcc
     3061 cgcgactccg tcggcgtccg tcccgtgctc caggcgattg aggcggccgg tctcgcctat
     3121 cgagacatcg acacgaagac cagctcgctc gaagaaatct tcgtcgacat catccgggaa
     3181 cagtcatgaa cgttcacgcc atcgaggcga tctaccgcta cgaaatggcg cggacgcgac
     3241 gcacgcttct tcaaagcgtc atcgcgccgg tcgtatcgac ctcgctctat ttcatcgtgt
     3301 tcggcgcggc gatcggcgcc cgcatgacga cgatcgacgg cgtcagctac ggcgccttca
     3361 tcgtgcctgg cctggtcatg ctgtcgctgc tgacgcaaag catcaccaat gcgtcgttcg
     3421 gcatctactt tccacgcttc gtcggcacga tctacgaggt gctctcggcg ccgatctccg
     3481 gaatcgaagt ggtgatcggc tatgtcggcg cggcggcgtc gaaatccatc ctgcttggcc
     3541 tgatcatcct ggcgacggcg acgctgttcg tgccgctgcg catcgagcat cctttctgga
     3601 tggcgctgtt tctcatcctc acggcggcga cgttcagcct gttcggcttc gtcatcggca
     3661 tttgggccga caatttcgag aagctgcagg tcgtgccgct gatcatcatc acgccgctca
     3721 cctttctcgg cggcagcttc tactcgatcg acatgctgcc gccgctctgg cggaagatcg
     3781 ccttggtcaa cccggtcgtc tatctgatca gcggcttccg ctggagcttt tacggcaagg
     3841 cggacgtcga tatccgcatc agcctcggca tgacgctgtt ttttcttgct ttgtgtctcg
     3901 ccgcagtggt ggcgattttc cggacgggat atcggctgaa gaaatgagcg cggcggcttg
     3961 cggggttttc aatctgcaca aaaaagccgc cccatcgggg gcggccaggt tgggccggag
     4021 caggccctag tcggttttga gaattagcgg cgctcgatgt cctctttagc ttgtcgggcg
     4081 gcgtctttcg cgtcgccggc gacgttgtgc gcggcgcctt tggctttatc gatcttccct
     4141 tcggcctgca atttcttgtc gtccatcaac ttgccggccg catccttgat ggcgcctttg
     4201 gccttgtccg ccgcgccttt cacatgttcg cgatccatcc tggctctcct ttcgagtgct
     4261 cacggcaagc aacgcgtcag gcggcggcgg gttcccgcgg ttcgtctcgc ctcggcgaag
     4321 aaagggccta tttggatcgt ggccttaaca tggcgctggg cgtcggtctt gggcgcaaat
     4381 aagcacaatt atcccagcgt atttttatag atgcgcccgt ccttcatcac gatccgcagc
     4441 gtctcgttcg gcgcgaccag gaagtcgagg ctcttcgtcg gatcgccgtc gacaataagg
     4501 agatccgcca tcgcgcccgg cgcgatgacg ccgaggtcgc cgtcgtaagg atagcgctgg
     4561 ccggaaaggc gcagtaactc gccgttccgc gcggtggcga tgcccagtag ctccaacggc
     4621 tcgtaaaagc gcggcgtgag cttggcgaga tggcgggcgt ggaacggcag attctgcggc
     4681 gcgaacagaa tgtccgttcc ccaggcggtt ttgattttgt actgtttcga gaattcgtac
     4741 gctctcgcgg ttccctggga aacgatttcg gccttggcgc gactagccgg atcaaccttc
     4801 ggattggagt cctcgtccga aaaaaacggc tgcagactcc accacacgcc ctcgcccgcc
     4861 atcatccgga cggattcttc gtcggtgagt tggccgtgct cgatcgactt cacgccggcg
     4921 cggatcgccc gctggatgcc gcgcggcgta tagacatggg tcatcacgta agtgccccag
     4981 tcctcggccg ccccgacccc ggcgcgcaat tcgctctcgc tgtactgcgt gacgtcgatt
     5041 ggatcgtagt gggaggagac gccgccgccc gccatcatct tgatctgcgt cgcgccgagc
     5101 aggagctgtt cgcgaacccg gcgcagcact tcggcctcgc cgtcggcgat aacgccgacg
     5161 ccggcggtct cggtcaggct gagcggcgtc tgcggaccgc gcggaatctc gtaccgctgt
     5221 cggaaatcgc catgacccgc cgtctgcgac accatcgcgc ctgacgggaa aatgcgcggc
     5281 ccggcgataa ggccggaatc gatcgcgcgt ttgagcgcga aagtcggacc gccggcgtcg
     5341 cgcaccgtcg tgaacccgcg cagcaaggtg cgctcggcct cgctcgcggc gaccaaatga
     5401 agatagggaa cgtcggcggt caatgcctcg gcataacgga tgccgcacag catggcgtgc
     5461 caatgcgcgt cgatgagacc cggcatggcc aggcggccgc cgcagtcgat cacctgcgcg
     5521 ccttccacgc tctcgcccgc tggcggcaat cccgcgaact tggcgccgcg cgctaggacg
     5581 ccgacccctt ggcgcaggcg tccgtcgagg ccgtcgaata gaagcaggtt ggaaaagaga
     5641 atgggccgtc cgggcgccgg cggcggctcg ctgagcgctt tggccggccg gagcccgatg
     5701 aacggcgcca gcagcgccgc cgctccgccg agcatcgcgc ggcgcgacag gtcggccgtg
     5761 atgcgccggt tcagggcccg cgcggccgga ccgcagcatt cgcaggcgga tgaccgcgaa
     5821 accgcgccgc cgccgtcgca agcaaagccg gcgcgcgccg gcggcgatgc tgagcttttg
     5881 gcaagaaacg gcggcctcat ccgaatctcc cggggagcgg tgtctacggc tccaattcgc
     5941 tgtcccagta gagatagtcc atccagcttt cgtgcaggta gttcggcggg aagagccggc
     6001 cgttctggtg caggtcgtgg atggtcggct gaaacggcgc tttggccggc cacatccgcg
     6061 ccgcggccgg cagcatgtcc cctttcctca agttgcagcg cgagcaggcg gcgaccacgt
     6121 tctcccaggt cgtcaggccg cccttcgacc ggggcacgac atggtcgaag gtcaattcgt
     6181 cgtcgtcgcc gcaatattgg caggtgaagc ggtcgcgcag gaacacgttg aaccgggtga
     6241 aggcgggata gcgcgagggc ttcacatagg tcttcagcga gacgaccgag ggcaggcgca
     6301 tttcgaatga cggactgcgc accgtccgct cgtattgcga aacgatgttg acccgatc
//
LOCUS       DATGJW010000181        11324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1278505, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000181 DATGJW010000000
VERSION     DATGJW010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11324
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1278505"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..434
                     /locus_tag="VKU03_05440"
     CDS             <1..434
                     /locus_tag="VKU03_05440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLJ70739.1"
                     /translation="SLIGDEPFFLCNTDAFWLEGPRSNLRRLAAAWDPDQMDVALLVA
                     ATVSAAGVDWPGDFSMHADGWLVRRQEREVAPYVYTGVGIIKPEFFKKDTRDVFRLAP
                     IFFEAAAKNRLYGVRLDGVWLHVGYPDAINEAERAIDRSTL"
     gene            557..1813
                     /locus_tag="VKU03_05445"
     CDS             557..1813
                     /locus_tag="VKU03_05445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856914.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HLJ70740.1"
                     /translation="MTTHQRNVVAAIDPVKLDRLAEAAVKVGLQLKAGQDLFLTSPVS
                     ALPLARRIAEHAYKAGAGLVTPILSDEEITLSRFRFARDESFDRAASWLYDGVAKAFA
                     ANTARLAIVGDDPMLLSQENPANVARANKANSLAYQPALEKIAGFDINWNIVAYPTAS
                     WAKRVFPGDDEHVAVAKLADAIFSASRVANDDPVAAWAAHNAALRNRTQWLNGQKFQA
                     LHYSGPGTDLTIGLADSHEWQGGASTAKNGITCNPNIPTEEVFTTPHARRVEGHVAST
                     KPLSYQGTLIDNIQVKFSEGRIVEAKAARGEEVLHKVLDTDDGARRLGEVALVPHSSP
                     ISQSGLLFFNTLFDENAACHIALGQCYSKCFIDGAKLSADEIATRGGNKSLIHIDWMI
                     GSDKIDIDGVHADGRRTPVFRKGEWA"
     gene            complement(1921..2586)
                     /locus_tag="VKU03_05450"
     CDS             complement(1921..2586)
                     /locus_tag="VKU03_05450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HLJ70741.1"
                     /translation="MGALARGVVYVALASFCIFIALVAVYSFAPPVSTLMVGRVVIGK
                     SYQRVYTPLKDIAPVVAASVIASEDGGFCRNAGVDWGALHEVLRKAGKNGPKRGASTI
                     TMQTAKNLFLWPGRSDIRKGIEIGMALVLGQAWSKSRTLEIYLNIAEWGDGIYGVEAA
                     AQRYFHKKARELTANEAALLVTSLPNPIKRDPAHPVGFQRRLAAAVAERARDEDLTSC
                     LGR"
     gene            2824..5916
                     /gene="addB"
                     /locus_tag="VKU03_05455"
     CDS             2824..5916
                     /gene="addB"
                     /locus_tag="VKU03_05455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HLJ70742.1"
                     /translation="MPAGAPFLASFADALLGGRLVAGFPGEGGPLALASATIYVPTRR
                     AARALSEALRKAGGGASLILPHITPLGAFEPTEDSLLFEPPDIAGEAPPAISDLERRM
                     VLARLAQAWSKALRGAILSINAGGEPVYHATEAPLVVAGPVQAFALAGDLAGLIDDMT
                     IEDIPWERLETLTPGEFDDYWRITLDFLKIAARHWPEHLRERGLIDEASRGAVLVAHE
                     IERLSQGRFGGPVIVAGSTGARRATAKLIAAVARAPQGAVVLPDLDFALDEESWRLIG
                     KQQIATHPQAGLSRLLETMEATRGDVIEIGAVSATLHSRARLISEALRPADSTELWRN
                     REDALAEQRINEALAGISVIEAADEAEEALTLAIAMREVLEIPGKTAALISPDLGLTR
                     RVKAELARWGVAVEDSAGGTLSATPAGVFARLILDAAATRKALDLLALLNNPLMRLKR
                     RRLMLEAAIRTLELGALRGVLPPTGLDDPARLFAEAKSKRREDDQRLKFDEDEWRAAE
                     ILLTDCLEAMAPLAALDAEAPLDAHIDAHARALATFNEDEDGYDRTTVSADGASLIAL
                     LAEWRAAAPADLMMDAANYRALFEAVLATTPAPDAARSKSRLQILGLLEARLLAFDMT
                     LLAGLDETVWPPAAETDAFLNRPMRAQLDLSPPERRIGQTAHDFVAALGAPQTILSRA
                     RKRGGAPTVASRFLQRIAAAAGDKAFGEAVKRGGAYLALARAIDRPDEVERARRPAPR
                     PPLALRPVKLSVTRIETLRRDPYAIFAERVLKLKPLDPIGQEAGAREIGVEWHDVFSK
                     FTTEFGVLPLPANARERLFDMAHENFAPLLEDDNFRLIAWPRIEQGLDFFLNFDRARR
                     SDLAQILTEKRGELAVPLANGSQFLLSATADRIEILKSGDIALVDYKTGAMPGVNEVR
                     VGFAPQLTLEAAMAARGAFDIAPPRAPIEALYLKLGGAKGGEERALKFKDEILADVAE
                     EHYRALIALLDQFADEATPYAPRPYPKFIGAFGVYDHLARVKEWSASGGLADSPGGEA
                     "
     gene            5913..9383
                     /gene="addA"
                     /locus_tag="VKU03_05460"
     CDS             5913..9383
                     /gene="addA"
                     /locus_tag="VKU03_05460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair helicase AddA"
                     /protein_id="HLJ70743.1"
                     /translation="MNAPVDEAVKSRRAGANPQARASDPKTSAWVTANAGSGKTHVLV
                     QRVLRLLLEGTPPSRILCLTFTKSAAANMSGRVFRTLARWTSLDDDALTKAITDAGAP
                     APDKDRLALARRLFARTIESPGGLKIETIHSFCGRLLRMFPFEANVAAGFRVLEERES
                     ELLRNDARTIALRRAAQDPAAAATLRNLAIETGGEGLDALLRQAQGFRTAINEAIEYW
                     GGLKAYDQALRARLGLAPNEDAEAVETSMLHGLGDKAAKLRVAAELDEGSVTDKKRAI
                     ALREAANLADRAEALAAYLTIFFTADGKPRGGDSGGLVTKALAKKFPLLDEQLRNEQS
                     RLIGLRERLKAARTVARSSGLIDIAERVLSAYARLKAERGLLDFDDLIERATALLIRA
                     DAAWIHYKLDSGIDHILVDEAQDTSHAQWEILRQLAEDFTSGKSAREKPRTFFAVGDD
                     KQSIFSFQGAAPEMFADMRRQLKKRHDDAGMPFEPVALTVSYRSSKVVLDSVDGVFAV
                     ENTWRGLTADEEPPPPHVAYRSHLPGLVEIWPTIAGEKETPDSDWRLPIDSPTSHDPP
                     VLLARRIAGVIADWLKPSSPERVYDSEAAMLRPIRPGDVLIMVRTRGAFFEAIIRALK
                     DANVRTLGADRLLLGDHIAVMDLVAAGKAALNPDDDLSLAATLKSPLIGLDDDDLIAL
                     APNRKASLARALADHPDEKYRAISRRLDAWRERARILEPYDFYARLLGEEGGRRAMLR
                     RLGPEASDAIEEFLSQALSFERRRAPSLLAFLAEFEDADVSIKRDMEESSEAVRVMTV
                     HAAKGLEAPIVFLPDTCSAPGGRDDGALFAIAATGANEPRFIVWSPRKAEDPALVAAA
                     REARREAARGEHRRLLYVAMTRAAERLIVAGYHGVRGAAEDCWSDMISAGLAEAAIET
                     PSPWSADETILRIGMGAREAALVLREEDEPPTSPPDWLTAPVSPEPEAAPLTAAPRRA
                     WARASSGEQRQTRLEAGRLSHTLLQYLPDIEPARRREAGRNYLAARSANIPASNQGAI
                     LDRTLAVLDDPRLALLFGPQSHAEVAIAAEFPRVGAPPTSFAGRIDRLAIAADHIVIA
                     DFKSGAPDPAHKTPADYVLQLALYRQALAPLYPGRPVRAYIVWLDLPQAIEIPPEALD
                     AAVVERLSLP"
     gene            9670..10899
                     /locus_tag="VKU03_05465"
     CDS             9670..10899
                     /locus_tag="VKU03_05465"
                     /inference="COORDINATES: protein motif:HMM:NF028178.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TAXI family TRAP transporter solute-binding
                     subunit"
                     /protein_id="HLJ70744.1"
                     /translation="MALSLPAFAEDAAQPDTSSADAQTTVQARPGMHRFMVRYARPVM
                     HNGKLVLWHGAWRNGAVHPRPMRAAATDDAATPDASKTAGAHSFSILADSSDPTATRL
                     ANELAGVMSGDDTQIKVLGAPASRAAIAKAVGADSADFALVTLDGLVDPAQSGVDWRR
                     KAPFVARLQNEEIEVIAPRAIANINQLSGRKVNVGAADSAAAGTAGLIFSHLSVPANW
                     TTYPLDDALQRLKDGKIDAVVVVGGKNSESLSKFGDDGRFHLVSIPYSPALRPYYAPA
                     RATKSDWPNLVAADEKVETLSTPIALVAIDGAVAERADRLAPAANKFLASFDQLLDDS
                     KDQGWRDVNLAARIDQLPRFGAAQAWLDQNKGEASADLEAFRAAAQAADAANGGPSGA
                     DSDKLYQSLTRLGGAAQ"
     gene            10914..>11324
                     /locus_tag="VKU03_05470"
     CDS             10914..>11324
                     /locus_tag="VKU03_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70745.1"
                     /translation="MSHMIRIGAGVSAVAVAIALGIAFWPQIDTSMAHGRIAVEKPIF
                     VVPAAPVKLEAALGRPQAAAAEPAAAPPTPPPATETAPAPSAAAGVTVVPGSQPASDD
                     DAPLAKLALAFRTNNGAAPGLGRSLTITPTGAPQA"
BASE COUNT         2054 a         3756 c         3534 g         1980 t
ORIGIN      
        1 tttcattgat cggcgacgag ccctttttcc tctgcaatac cgacgcattt tggctcgagg
       61 ggccgcgctc caatctccgg cggctcgccg cggcctggga ccccgaccaa atggacgtcg
      121 cgcttcttgt cgccgcgacg gtcagcgcgg ccggcgtcga ctggcctggc gatttctcga
      181 tgcatgccga cggctggctg gtgcgccgtc aggagcggga agtcgcgccc tatgtctata
      241 ccggcgttgg catcatcaaa ccggagttct tcaagaagga tacgagagac gtcttccggc
      301 tggcgccgat tttctttgaa gccgccgcca agaaccgcct ctatggcgtg cgcctcgacg
      361 gcgtctggct gcatgtcggc tatcctgacg caatcaacga ggcggagcgc gcgattgatc
      421 ggtcgacctt gtaaaggagc ggccttgaag cagccgttct ttggaaatga gattgacggc
      481 ttaagtttac gccaggccgc cgcgcgccgc tggcccgaga aacaaaagcg aagataaaaa
      541 cctcaaggca aatttcatga ccacgcatca acgcaatgtc gtcgccgcca tcgaccccgt
      601 aaaactcgat cgcttggccg aagcggccgt caaagtcggc cttcagctca aggctgggca
      661 agatcttttc ctcacctcgc cggtatcggc gctgcctttg gcgcgccgga tcgcggaaca
      721 cgcctataag gctggcgcgg gtctcgtgac gccgattctg tccgacgagg aaatcacgct
      781 gtcgcggttt cgctttgcgc gcgacgagag tttcgaccgc gccgcgagct ggctttacga
      841 cggcgtcgcc aaagccttcg ccgccaatac ggcgcggctc gcgattgtcg gcgatgatcc
      901 gatgctgctc tcgcaagaga atccggccaa tgtcgcgcgc gccaacaagg ccaattccct
      961 ggcgtaccag ccggccttgg agaagatcgc gggtttcgac atcaattgga acatcgtcgc
     1021 ctatccgacc gcctcctggg cgaagcgcgt ctttcccggg gatgacgagc atgtcgcggt
     1081 ggcgaaactc gccgacgcga ttttttcggc ctcgcgcgtc gccaacgacg atcccgtcgc
     1141 cgcctgggcc gcgcataatg cggccttgcg caatcgcacc caatggctca acggccaaaa
     1201 attccaggct ttgcattatt ccgggccggg cacggatctc acgatcggcc tcgccgatag
     1261 ccatgaatgg cagggcggcg cctcgaccgc caaaaacgga atcacctgca atccgaacat
     1321 tccgaccgaa gaggtcttca cgactccgca cgcaaggcgc gtcgaaggcc atgtcgccag
     1381 caccaagccg ctttcctacc agggcacgct gatcgacaat atccaggtga aattcagcga
     1441 aggacggatc gtagaagcca aggccgcgcg cggcgaagaa gtcctgcaca aagtgctcga
     1501 caccgacgat ggcgcgcgcc gcctcggcga agtcgccctc gtgccgcatt cctcgccgat
     1561 ctcgcaaagc ggtttgcttt tcttcaacac gctcttcgac gaaaacgccg cctgccatat
     1621 cgcgctcggc cagtgctatt ccaaatgttt catcgacggc gccaagctca gcgccgacga
     1681 gattgcgacc cgcggcggca acaagagcct catccatatc gattggatga ttggctcgga
     1741 caagatcgac attgacggcg tccatgccga cggacgtcgt acgccggtgt tccgcaaagg
     1801 cgaatgggcg tagccttcga gtcattgcga agagcgaaag cgacgaagca atccattcaa
     1861 agggcgccgc ctgggaaagg attgcttcgc tttgctcgcg atgacgcgct aactcgctgc
     1921 ctaacgccct aagcaactcg tcaaatcctc gtcccgcgcg cgctcggcga cggcggcggc
     1981 gagtcggcgc tggaagccga cgggatgggc gggatcgcgt ttgatcggat tcggcagcga
     2041 ggtcacaagc agcgccgctt cattcgccgt gagctctctc gcctttttgt gaaagtaacg
     2101 ttgggcggcg gcttcgacgc cgtagatgcc gtcgccccat tcggcgatgt tgagatagat
     2161 ttccagtgtc cgcgatttcg accaggcctg gccgagcacg agcgccatgc caatctcgat
     2221 tcccttgcgt atgtccgagc ggcccggcca gaggaaaaga ttcttcgccg tttgcatcgt
     2281 gatggtggag gcgccgcgct tcgggccgtt cttgccggcc ttgcgcaaca cttcgtgaag
     2341 cgcgccccaa tcgacgccgg cgttccggca gaagccgcca tcctcggagg cgatcaccga
     2401 ggcggcgacg accggcgcaa tgtcttttag gggagtatag acgcgctgat agcttttgcc
     2461 gatgacgaca cgtccgacca tgagcgtcga aaccggcggc gcgaaactat agacggcaac
     2521 cagcgcgata aaaatgcaga aggacgccag cgccacatag acgacgccgc gcgccaaagc
     2581 gcccaaggaa gatcgcggcg aagccatacg cgcagtgttt gccaacccgc cgtgtcaggc
     2641 aaggggacgt tacgttgaac cagcgttagt tcaaaaccca tgagaacgcg gcggattttt
     2701 cctcgtccgc atgggtggcg cgtagctctt gccacccgcc gattcgcgcc gcaaaacacc
     2761 ccttacgaat cggccgttcg acgctttagc gaggcccctc tgcccgacct aaaactcttc
     2821 tcgattccgg ccggcgcgcc ttttctggcg agcttcgccg acgccttgct cggcggccgg
     2881 ctggtcgcgg gctttccggg cgaaggcggg ccgctcgctc tggcgagcgc cacgatctat
     2941 gtgccgacgc gccgcgccgc ccgggcgctt agcgaagctt tgcgcaaagc gggcggcggc
     3001 gcgagtctga tactgccgca catcaccccg ctcggcgcct tcgagcccac cgaggacagt
     3061 ctgctcttcg aaccgcctga cattgccggc gaggcgccgc cggcgatttc cgatctcgag
     3121 cgccgcatgg tcctggcgcg gctcgcccag gcgtggagca aggctttgcg cggcgcgatc
     3181 ctctcgatca acgcgggggg cgagccggtc tatcacgcga ccgaagcccc gctcgtcgtc
     3241 gccggtcccg tccaggcttt cgcgctcgcc ggcgatctcg ctggcctcat cgacgacatg
     3301 acgatcgagg acattccctg ggagcgcctg gagacgctga cgcctggcga gttcgacgat
     3361 tattggcgca tcacgctcga ctttctcaag atcgcggcca gacattggcc agagcatttg
     3421 cgcgaacgcg gattgatcga cgaggcctcg cgcggcgccg tgctcgtcgc gcatgaaatc
     3481 gaacgcctca gccaaggaag gttcggcggt ccggtgatcg tcgccggttc gaccggcgcg
     3541 cggcgcgcga cggccaaatt gatcgccgca gtagcgcgcg cgccgcaagg cgccgtcgtt
     3601 ctgccagacc tcgactttgc gctcgacgaa gagtcctggc ggctgatcgg caagcagcaa
     3661 atcgcgacgc atccgcaagc cggcctgtcg cgcctgctcg aaacgatgga ggcgacgcgc
     3721 ggcgacgtca tcgagatcgg cgccgtctcc gccacgctgc attcccgcgc gcgcctgatc
     3781 agcgaagcgt tgcgcccggc cgattcgaca gagctttggc ggaaccgcga agacgcgctc
     3841 gccgagcagc gaatcaatga ggcgctcgcc ggcataagcg tcatcgaagc tgcggacgaa
     3901 gccgaagaag cgctcacttt ggccatcgcc atgcgtgagg tcttggagat tccagggaag
     3961 accgcggcgc tgatttcgcc tgaccttggc ctgacccgcc gcgtcaaagc cgaactcgcg
     4021 cgctggggcg tcgcagtcga agattccgcc ggggggacat taagcgcgac ccctgccggc
     4081 gtcttcgcgc ggctgattct cgacgcggcc gcgacgcgca aagcgctcga tctcctcgcg
     4141 ctgctcaaca atccgctcat gcgcctcaag cgccgacgcc tgatgctaga ggccgcgatc
     4201 cgtaccctcg aactcggcgc gctccgtggc gttctcccgc cgaccggcct cgacgatccg
     4261 gcgcgtctct ttgcagaggc caaaagcaag aggcgcgagg acgatcaacg cttgaagttc
     4321 gacgaagacg aatggcgcgc ggctgagatt ctgctgaccg attgcctgga ggctatggcg
     4381 ccgctcgcgg cgctcgacgc cgaggcgccg ctcgacgccc atatcgacgc tcacgcgcgc
     4441 gctctcgcga ccttcaacga agacgaagac ggatacgata gaacgacggt aagcgcggac
     4501 ggcgcttctc tcatcgcctt gctcgcggaa tggcgcgccg cggcgccggc cgatttgatg
     4561 atggacgcgg cgaactatcg agccctgttc gaagccgtgc tcgccacgac gccggcgccc
     4621 gatgcggcgc gatcgaagag ccgtctgcaa atcttaggtc tcctcgaagc gcgccttctc
     4681 gccttcgaca tgaccttgct cgcagggctc gatgagacgg tctggccgcc cgccgccgag
     4741 accgacgctt tcctcaaccg gccgatgcgc gcgcaactcg acctatcgcc gccggagcgc
     4801 cgcatcggcc agacggccca tgattttgtc gccgcgctcg gcgcgccgca gacgatcctt
     4861 tcccgcgctc gaaaacgcgg cggtgcgccg acggttgcgt cgcggttcct gcaacgcatc
     4921 gccgcggcgg ccggggacaa agcctttggc gaggcggtca agcgcggcgg cgcctatctc
     4981 gcgctcgccc gcgccatcga tcgccccgat gaagtcgaaa gggcgcgccg gccggcgcca
     5041 agacccccgc tggcgctgcg gccggtcaaa ctcagcgtca cccgcatcga aactttgcgg
     5101 cgcgatcctt acgcgatctt cgccgagcgc gttctcaaac tgaagccgct cgatccgatc
     5161 ggtcaggagg ccggcgcgcg cgaaatcggc gtcgaatggc acgacgtctt cagcaaattc
     5221 acgaccgagt tcggcgttct gcctttgccc gccaatgcgc gagaacgcct gttcgatatg
     5281 gcgcacgaaa atttcgcgcc cctcttggag gacgacaatt tccgtctcat cgcctggccg
     5341 cgcatcgaac agggactgga cttttttctc aatttcgatc gcgcccgacg cagcgacttg
     5401 gcgcagattc taacggagaa gcgcggcgag ctcgccgtgc ccctcgccaa tggctcgcaa
     5461 ttcctcctct cggcgacggc ggaccgcatc gagattctca agagcgggga catcgcgctt
     5521 gtcgactaca agaccggcgc gatgccgggc gtcaatgaag tgagggtcgg cttcgcgccg
     5581 caattgacgc tcgaggcggc gatggcggcg cgcggcgctt tcgacatcgc gccgccgcgg
     5641 gcgccgatcg aagcgcttta tctcaaactc ggcggtgcga aaggcggcga agaaagagcg
     5701 ctgaaattca aggacgaaat cttggccgat gtcgccgagg agcactatcg cgccttgatc
     5761 gcgctgctcg atcaattcgc cgacgaggcg acgccctatg cgccgcggcc ctatccgaaa
     5821 ttcatcggcg ctttcggcgt ctatgatcat ctcgcgcggg tcaaggaatg gtcggcctcc
     5881 ggcggccttg ccgatagccc gggaggcgag gcttgaacgc gccggtcgac gaagccgtta
     5941 aaagtcgccg agccggcgca aatccgcagg cgcgcgcctc cgaccccaag acgtcggcct
     6001 gggtcaccgc caatgcgggc tcgggaaaaa cccatgtctt ggtacagcgc gttctgcgcc
     6061 tcctgctcga aggtacgccg ccttcccgca ttctttgcct caccttcacg aagagcgcgg
     6121 cggcgaacat gtccggccgc gtcttcagga ccctggcgcg ctggaccagc ctcgacgacg
     6181 acgcactcac caaggccatc accgacgccg gcgcgccggc cccggataaa gatcgcctgg
     6241 ccttggcgcg ccgccttttc gcgcgcacga tcgagtcgcc gggcggcctg aaaatagaga
     6301 cgatccatag tttctgcggc cgtctgcttc ggatgtttcc attcgaggcc aatgtcgcgg
     6361 caggttttcg cgtcctcgaa gagcgcgaaa gcgaattgtt gcgaaacgac gcgcggacaa
     6421 tcgcccttcg gcgggcggcg caagaccccg ccgcggctgc gacgctgcga aatctcgcga
     6481 tcgagacggg cggcgaaggc ctcgacgctt tgctgcgcca ggcgcagggc tttcgcacag
     6541 cgatcaacga ggcgatcgaa tattggggcg gtctcaaagc ttacgaccaa gccttgcgcg
     6601 ctcgtctggg cctcgcgccc aacgaggacg ccgaggccgt cgagacgtcg atgctgcacg
     6661 gccttggcga caaggccgcg aaactaaggg tcgccgccga gctcgacgag ggatccgtca
     6721 ccgacaaaaa gcgcgctatc gcgttgcgcg aggcggcaaa tctcgccgat cgcgccgaag
     6781 cgctggccgc ttatctcacg attttcttta ccgcagacgg caagccgcgc ggcggcgaca
     6841 gcggcggact cgtcaccaag gcgctcgcca agaaattccc ccttctcgat gaacagcttc
     6901 gcaacgaaca aagccgcttg atcggcttac gcgaaaggct gaaggccgcc cgcacggtgg
     6961 cgcgcagcag cggcttgatc gatatcgcgg agcgcgtgct ttccgcctat gcgcgcttga
     7021 aagctgagcg cggcctgctc gatttcgacg atctcatcga acgcgccacc gccttgctca
     7081 tacgcgcgga tgcggcctgg attcactaca agctcgattc cggcattgat cacattctcg
     7141 tcgacgaggc gcaggacacc tcgcacgccc aatgggaaat tttgcgccaa ctcgccgagg
     7201 acttcacgtc aggcaaaagc gcgcgcgaga agccgcgaac tttcttcgcg gtcggcgacg
     7261 acaagcaatc gatcttctcc tttcaaggcg ccgcgccgga aatgttcgcg gacatgcggc
     7321 ggcaattgaa aaaacgccac gacgacgcgg gcatgccctt cgagccggtc gcgctgaccg
     7381 tttcgtaccg ctcgtccaag gtcgttctcg atagcgtcga tggcgttttc gcggttgaaa
     7441 atacctggcg cggccttacc gccgacgagg agccgccgcc gccgcatgtc gcctaccgtt
     7501 ctcaccttcc cggtctcgtc gaaatctggc cgacgatcgc gggcgaaaaa gaaacgcccg
     7561 attccgactg gcggttgccg atcgactcgc cgacaagtca cgatccgcct gtgctgcttg
     7621 cgcgacgcat cgccggcgtc atcgccgact ggctgaagcc ctcatcgccg gagcgcgttt
     7681 acgattccga agccgcaatg ctgcggccaa tccggccggg cgacgtcctc attatggttc
     7741 gcacgcgcgg cgcttttttc gaagcgatca ttcgggctct gaaagacgcc aatgtcagga
     7801 cgctcggggc cgatcggcta ttgctcggcg accatatcgc cgtcatggac cttgtcgcgg
     7861 ccggcaaggc ggcgctcaac ccagacgacg atctttctct cgccgcgaca ttgaaatcgc
     7921 cgctcatcgg ccttgacgac gacgatctca tcgcgctggc gccaaatcgc aaggcttcgc
     7981 tcgcccgggc gctcgcggac catcccgacg aaaagtaccg cgcgatttca cgccggctcg
     8041 atgcatggcg ggagcgcgcg cgaattctcg aaccctatga tttctatgcg cgcttgctcg
     8101 gcgaggaggg cgggcggcga gcgatgctgc gccgactcgg cccggaggcc agcgacgcaa
     8161 tcgaggaatt tctgtcacag gctttgtctt tcgaacgaag acgcgcgccc tcgctcctcg
     8221 ccttcctcgc tgaattcgaa gacgccgacg tctcgatcaa gcgcgacatg gaggaaagca
     8281 gcgaggccgt gcgggtcatg acggtgcatg cggccaaggg cctcgaggcg ccgattgttt
     8341 tcttgcccga cacttgttct gcgccgggcg gccgcgacga tggggcttta ttcgcgatcg
     8401 ccgcgacagg cgcgaatgaa ccgcgcttca tcgtctggtc gccgcgtaaa gccgaggatc
     8461 cggcgctggt cgccgcggcg cgggaggcta ggcgcgaagc ggcgcgcggc gagcaccgcc
     8521 gccttctcta tgtcgcgatg acgagagccg ccgaacggct gatcgtcgcc ggataccacg
     8581 gcgtcagagg cgccgcagag gattgctgga gcgatatgat ctcagccggt ctcgccgaag
     8641 ccgcgatcga aacgccgtcg ccgtggagcg ctgacgagac gatcctgcgt atcggtatgg
     8701 gcgcgcgcga agcagcgctt gtcctacgcg aagaggacga gccgccgaca tcgccgcccg
     8761 attggctcac agcgcctgtc tcgcccgagc cggaggcagc cccgctgact gctgcgccgc
     8821 gcagggcttg ggcgcgcgct tccagcggcg aacaacgcca gacccgtctc gaggctggac
     8881 gcctcagcca tacgctgctg caatatctgc cggacattga gcctgcgcgc cgtcgagagg
     8941 ccggacgaaa ctatcttgcc gcgcgcagcg caaatattcc ggcgagcaat caaggcgcta
     9001 ttctcgatcg cacgctcgcc gtactcgacg acccgcgtct cgctctgctc ttcggccctc
     9061 aatcgcatgc cgaagtcgcc attgcagccg aatttccgcg agtgggcgcg ccgccgacgt
     9121 ccttcgccgg aaggatcgac cgtctcgcga tcgccgcgga ccatatcgtc atcgcagatt
     9181 tcaaaagcgg cgcgccggac cccgcccaca agacgccggc cgattatgtg ctgcaactcg
     9241 cgctttaccg gcaggccttg gccccgcttt atcccggccg tcccgtacgc gcctatatcg
     9301 tttggcttga tctgccgcag gcgatagaaa tcccgcccga agcgctcgac gccgctgttg
     9361 ttgagcgcct ttcgttgcct taatccaata actcttttat ttcataaggt tacgtgctac
     9421 taaggccaaa gatggatatt ttttcgccga atcggaactt tcccgccatt aacgccgaga
     9481 gattgcattc gcgttaagcc cgccttgaag tggacgcatt ttcaaaggat tgcttggcca
     9541 atgacgagtg gagttccgcg attgcatgcg cctttgcgct cgattgcgca ggcgaaaagg
     9601 ggaccgtggc gagccgggcg tggcgaggcc gaacgggcac gttgcgtcta tttggcgcta
     9661 ggtctcgctt tggctctgag ccttccggcc tttgccgagg atgctgctca gcccgacacg
     9721 tcgagcgcag acgcccagac caccgtccag gcccgtccgg gcatgcaccg atttatggtg
     9781 cgctatgcca ggccggtgat gcacaacggc aagctcgtgc tttggcatgg cgcctggcgc
     9841 aatggcgcag tccatccgcg cccgatgcgc gccgcggcta ccgacgacgc ggcgacgccg
     9901 gatgcgtcga aaacggccgg cgcgcatagt ttttcaatcc tcgccgatag cagcgacccg
     9961 accgcgacgc gattggcgaa tgaactcgct ggcgtcatga gcggcgatga tacgcaaatc
    10021 aaagtgctag gagcgccggc ctcgcgcgcg gcgatcgcca aggcggtcgg cgccgattcc
    10081 gcggatttcg ctcttgtcac gctcgacggc ttggtcgacc ccgcccagag cggcgtcgat
    10141 tggcggcgca aggcgccctt cgtcgcccgg ctgcaaaacg aagaaatcga agtgatcgcg
    10201 ccgcgggcga tcgccaatat caatcagcta tccggacgca aggtgaatgt cggcgcggct
    10261 gacagcgccg ccgccgggac cgccggcctc atcttctccc atctcagcgt accggcgaat
    10321 tggacaacct atccgctgga cgacgcgctg cagcgcctga aggacggcaa gatcgacgcc
    10381 gttgtcgtcg tcggtggtaa gaactcggaa tccctatcga aattcggcga cgacggccgc
    10441 ttccatttgg tgtcgattcc ttattcgccg gccctgcgtc cgtactatgc gccggcgcgg
    10501 gcgacgaaga gcgactggcc gaatcttgtc gcggccgatg agaaggtcga gacgctttcc
    10561 acgccgattg cgcttgtggc gatcgacggg gcggttgcgg agcgcgccga ccgcctggcg
    10621 ccggcggcga acaagtttct cgccagtttc gaccaattgc tcgatgattc gaaggatcaa
    10681 ggatggcgcg acgtcaatct cgccgcgcgc atcgatcaat tgccgcgttt cggcgctgcg
    10741 caagcttggc tcgaccagaa caagggcgaa gcaagcgctg atctcgaggc ctttcgagcg
    10801 gcggctcaag ccgccgacgc ggcgaatggc ggcccaagcg gagcggattc cgataaattg
    10861 taccagagcc tgacacgctt gggcggggcg gcgcagtgat tgggagtgcc ggcatgtccc
    10921 acatgatcag aatcggcgcg ggcgtcagcg cagtcgcggt ggccatcgcg ctcggcatcg
    10981 ccttttggcc acagatcgat acgtccatgg cccatggacg gattgcggtg gagaagccga
    11041 tctttgtcgt tccggccgcg ccggtgaaac tcgaggccgc cctgggacgt ccgcaggcgg
    11101 cggccgcaga gcctgcggcc gcgccgccta cgccgccgcc ggcgacggaa accgcgcctg
    11161 cgccctctgc cgccgccggc gtaacggtcg tcccaggatc acaaccagcc agcgacgatg
    11221 acgcgccgct ggccaaactc gcgctggcgt ttcgcaccaa taacggggcc gcgccgggcc
    11281 ttggcaggag cttaacaatc acgcccactg gcgcgccgca ggcg
//
LOCUS       DATGJW010000182         3190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1172407, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000182 DATGJW010000000
VERSION     DATGJW010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3190
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1172407"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VKU03_05475"
     CDS             complement(<1..446)
                     /locus_tag="VKU03_05475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLJ70746.1"
                     /translation="MSPIAFLLDEAWKRPALWERLRRAIRLTGYDTTDWLRTVMYREA
                     FRHVETLKPETLDALEISAGNQWSRAFRFRSYEATAYPAFDICAEILPRQFDLVIADQ
                     VFEHLKWPYRAGRNVYDMLKPGGTFILATPFLVRVHRVPIDCSRWT"
     gene            complement(475..1986)
                     /locus_tag="VKU03_05480"
     CDS             complement(475..1986)
                     /locus_tag="VKU03_05480"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLJ70747.1"
                     /translation="MGRSPSAAAEGTIHAAPAIGGGRRLAVNALAGGGANALKIGVQL
                     VMLPLMAHLLGPGEFGLYALALPMVSFCMILADGGLAASLAREPLESVLVWSSAFWLV
                     LAVGFALAVVAIGWGVALAALSREPRVSSLMCILSISLLMISAAALPSARLTREARLT
                     VFAAADVVSTLLGASVAVILAISGFGAKSLAAQYVTYHTLRAIILNATAFVRPQWRFQ
                     LSDLSNHLSVGSALLGVKMSDFFGRMIENVLYGRAFGAAGLGAYAFANQAPRFICEAA
                     SGPVWAALYAFALREKPDRLLAMHVRLVRLLSSLVFPIAALLSASAPQILDLILGPRW
                     SDAGELLRLLIPFYALGVVSGQTGAVLLAKGRGWLLFWLSLFLAVGRIAAVAAGPLVG
                     QAGVALGVGVALAFFSLAMFSTAGHPGGARRLLGAPVSPILAAALAGTAAFALLRAAE
                     PSVWLLALAWGGGGLVYIATLALMQGRTLASDIKTARSALAGFRQAGTKKRTT"
     gene            2090..>3190
                     /locus_tag="VKU03_05485"
     CDS             2090..>3190
                     /locus_tag="VKU03_05485"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ70748.1"
                     /translation="MTRKDGKFLSDGFDATERDIRVFVHLARDKDADAWRAAKAAGRL
                     VGVNEDTPYGYGRAERMGCAIAFSHSHRETVAGAFLRIAARVALGFDFLHAWRQRTAI
                     AGADIVWTHTESQYLAVAAVARAIGARPRLIGQTVWLMDRWPSLSFLHRALFRRLARD
                     IDVLTFHSPLNLKSAEKAFPGQRLELVRFGVPAETTAAPRRREGRPIRVLALGNDSHR
                     DWPCLIEAVAKAEDMNLRILSRAVPRRLARGTRNIEIGLARTQDELIAAFREATVVCT
                     PLKPNLHASGITVIQEAVLAGVPVVATDTGGLDAYFARDEITFVPPSDAVALRAALRK
                     LAADPDAALAQAQRAQARMRQGELGAEAYIRRH"
BASE COUNT          644 a         1040 c         1025 g          481 t
ORIGIN      
        1 tcggtccagc gcgaacaatc gatcggcacg cggtgcacgc ggacgagaaa aggcgtcgcg
       61 agaatgaagg ttccgcctgg tttaagcatg tcatagacat tgcgcccggc gcgatagggc
      121 catttgagat gctcgaacac ctgatcagcg atgacgagat caaattgccg cggtagaatc
      181 tcggcgcaaa tgtcaaaagc cggatatgcg gtggcctcat aactgcggaa gcggaacgcg
      241 cggctccact gattgccggc cgaaatctca agcgcgtcga gcgtctcggg ctttagagtt
      301 tcgacatggc ggaacgcctc gcgatacatg actgtacgca gccaatcggt ggtgtcatag
      361 cccgtaaggc gaatagcgcg gcgcaagcgc tcccacagtg cggggcgctt ccaagcttcg
      421 tcgagcaaaa aagcgatcgg actcatacta atctcatcgc tcgcgagtcg cggctcaagt
      481 cgtacgtttt ttcgtcccgg cttgtctgaa gccggcgagc gccgaccttg ccgttttgat
      541 atcggaggca agggtgcgac cctgcatcag cgctagcgtg gcaatataaa cgaggccgcc
      601 gccgccccag gctagcgcga gcagccaaac gcttggctcg gcggcgcgca agagcgcgaa
      661 tgcggccgta cccgcaagcg cggccgccag gatcggcgat acgggcgcgc ctaacaatcg
      721 gcgcgcgccg ccgggatgac cggccgttga aaacatcgcc agcgaaaaaa aagcgagcgc
      781 aacgcccacg ccaagcgcaa cgccagcctg gccgaccagc ggtcccgcag cgacggcggc
      841 gatccggcca acggctaaaa acagcgacag ccagaacaat aaccaaccgc ggcccttggc
      901 gagcagcacc gcgccggttt ggccgctcac gacgccaagc gcataaaagg gaatcaaaag
      961 ccgcagcagt tcgccggcgt cggaccatct cggtccgagg atcaagtcga gaatttgtgg
     1021 cgccgaggcc gacaagagcg cggcgatcgg aaaaacgagg ctcgatagca agcgcacgag
     1081 cctgacgtgc atcgccagta ggcgatcagg cttttcgcgc agcgcgaagg cgtaaagcgc
     1141 ggcccagacg gggcccgacg ccgcctcgca aatgaagcgc ggcgcctgat tggcgaaggc
     1201 gtaggcgccg agccccgccg ccccgaacgc gcggccatag agcacattct cgatcatgcg
     1261 accaaaaaag tcggacattt tcacgccgag cagcgcgctg ccgaccgaca gatggttcga
     1321 aagatcggag agctggaagc gccattgcgg ccgcacgaag gcggtcgcat tgagaatgat
     1381 cgcgcgcagc gtgtgatagg tcacgtattg cgccgcgagg ctcttggcgc cgaaaccgga
     1441 tatcgcaaga atcacggcga cgctcgcgcc gagcaaggtc gagaccacgt cggccgccgc
     1501 gaacactgtc aaccgcgcct cgcgggtcag tcttgcgctc ggcagcgccg ctgcagaaat
     1561 cataagcagg ctgatcgaca aaatacacat gaggctgctg actcgcggct cgcgcgataa
     1621 cgctgcaagc gcgacccccc agccgatcgc gacgaccgcc agcgcgaaac cgacggccag
     1681 aacgagccaa aaggcggacg accagaccag gaccgattcg agcggttcgc gcgcgagcga
     1741 agccgccagg ccgccatcgg cgagaatcat gcagaacgag accatcggca aagccagcgc
     1801 gtacaggccg aattcgcccg gccccaagag atgcgccatc agcggcagca tgacgagttg
     1861 cacgccgatc ttaagcgcgt tggcgccgcc gccggcgagg gcgttgacgg cgagccgacg
     1921 cccgccgccg atggccggcg ccgcgtggat tgtcccttcg gccgccgccg atggagacct
     1981 gcccatgatc cttatcccgt ctacacgacg ggcgcgcgct cgcgcccgcc ccgaaagccc
     2041 gcctgcccta tccgggtcac agtcatgtcc caatacgaat aaacctcgaa tgacacgcaa
     2101 ggacggcaag tttttgtccg acggcttcga cgcgacagag cgagatattc gcgttttcgt
     2161 gcatctggcc cgcgacaagg acgcagacgc ctggcgcgcg gccaaggcgg cggggcgatt
     2221 ggtaggcgtc aatgaggaca cgccctatgg ttacggtcgc gccgaacgca tgggctgcgc
     2281 catcgcgttt tcgcattcgc accgggaaac ggtcgccgga gctttcttgc ggatcgcggc
     2341 gcgcgtcgct ctcggtttcg atttcctgca tgcctggcga cagcgcacgg cgatcgcagg
     2401 cgccgatatc gtgtggacgc atacggaatc gcaatatctc gccgtggctg cggttgcgcg
     2461 cgcgatcggc gcgcggccgc gcctcatcgg ccagaccgtc tggctgatgg accgctggcc
     2521 atcgctgagc ttcctgcatc gggccttgtt ccggcgcctg gcgcgcgaca tcgacgtgtt
     2581 gaccttccat tccccgctca atctcaaatc tgcggagaag gcgtttcccg gccagagact
     2641 cgaactcgtg cgtttcggcg tccccgccga gacgacggcg gcgccgcggc ggcgggaagg
     2701 gcggccaatt cgcgttctcg cgctcggcaa tgacagccat cgcgattggc cgtgtctgat
     2761 cgaggccgtc gcgaaggcgg aggacatgaa cctgcgtatt ctctcccgcg ccgttccgcg
     2821 acggctggcg cgcgggactc gcaatatcga gatcgggctc gcccgtactc aagacgagct
     2881 tatcgccgcc tttcgcgagg cgactgtcgt ttgtacgccg ctgaagccga acctgcacgc
     2941 gagcggaatc acggtgatcc aggaggcggt gctggcgggc gtcccggtgg tcgcgacaga
     3001 tacgggcggc ctcgatgctt atttcgcccg cgacgaaatc acgttcgtcc ctcccagcga
     3061 cgccgtcgcc ctgcgcgccg ccctgcgcaa gcttgccgcc gatcccgatg cagcgctggc
     3121 gcaggcccaa cgcgctcagg ctcggatgag gcagggagaa ctcggcgcgg aagcctatat
     3181 ccgcagacat
//
LOCUS       DATGJW010000183        11057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_345932, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000183 DATGJW010000000
VERSION     DATGJW010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11057
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_345932"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..163
                     /locus_tag="VKU03_05490"
     CDS             <1..163
                     /locus_tag="VKU03_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158622.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HLJ70749.1"
                     /translation="DEHEEGPSAMLRKREDSDRDDRGDRRGGRPDRGDRDRGDRPRRP
                     REDAQPGAL"
     gene            164..400
                     /gene="rpsR"
                     /locus_tag="VKU03_05495"
     CDS             164..400
                     /gene="rpsR"
                     /locus_tag="VKU03_05495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HLJ70750.1"
                     /translation="MSTAPRRPFFRRRKTCPFSGPNAPKIDYKDTRLLSRYISERGKI
                     VPSRITAVSAKKQRELAAAIKRARFLGLLPYVIR"
     gene            538..1539
                     /locus_tag="VKU03_05500"
     CDS             538..1539
                     /locus_tag="VKU03_05500"
                     /inference="COORDINATES: protein motif:HMM:NF021506.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2232 domain-containing protein"
                     /protein_id="HLJ70751.1"
                     /translation="MVQRIAIALGAGMVAALLFAVMAKGTPVAMALAYLAPLPIVIAA
                     FNWGPDMGAVAAVTACGGAAALVDPYSGALFGAAIALPAWLLSALSSAHVGIFRRAAK
                     DQEQTAVWCPIGLVVTAAAVIGALIGLSALISLIVLYGGYQKGVEELVRQFTADIRDA
                     LDQMIALPVGMTVEEFATLVVRMSPPMLAAAIFLMLCANLYVGARVAQISQTLKRPWP
                     NLPEALVLPQPLGVALVLCLGLALTLGDPLRQAAWIGVGAFAAAYALQGLALAHGLTR
                     GLQHRNPLLFALYLAVAFAPRWVVPAVTMAGLLESFLALRAKRVAAANANNLNQRKP"
     gene            1553..2119
                     /gene="rplI"
                     /locus_tag="VKU03_05505"
     CDS             1553..2119
                     /gene="rplI"
                     /locus_tag="VKU03_05505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HLJ70752.1"
                     /translation="MEVILLERIARLGQMGDTVRVKDGFARNFLLPRGKALRATEANR
                     KKFEGQRQQLEARNLELKREANDVSVKLDGQSFVIIRQAGETGQLYGSVSSRDIAEAA
                     TAAGFTINRNQVVLLHPIKTLGLHKTPIQLHPEVTATVTINVARSQEQAERQAKGEDL
                     TVREETNLDDLGLEVGAALAEAAPPPQG"
     gene            complement(2235..3245)
                     /locus_tag="VKU03_05510"
     CDS             complement(2235..3245)
                     /locus_tag="VKU03_05510"
                     /EC_number="5.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016857 - racemase and epimerase activity,
                     acting on carbohydrates and derivatives [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldose epimerase family protein"
                     /protein_id="HLJ70753.1"
                     /translation="MNIRAFGEAEGVPIVEITIASKAGAVAKILNWGAVVRDLIVPSK
                     NGPQRVTLGLNSLADYEAHSPSFGAVPGRFANRIANGRFSLDGVDYELDRKPGEKHTL
                     HGGPHGFGKRPWRLANHDETSVTLTLHSADGDSGFPGALEATCVYRIEEPSTLRVELT
                     AVSDRPTIVNLTQHAYFNLDGSADILDHELTLNADFYTPTDADLIPTGEIRAVAGTPY
                     DFRSARPVRHASGQTYDTNFVLAARILADGLAPAARLRSPKNGLTLDLFTTEPGVQFY
                     DAAKLNCPVPGLDGAHYGPHAGLCLEPQVYPDSPNRRHFPDCVLRPDEEYRHVSAFRF
                     SY"
     gene            complement(3342..4646)
                     /locus_tag="VKU03_05515"
     CDS             complement(3342..4646)
                     /locus_tag="VKU03_05515"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLJ70754.1"
                     /translation="MASPAALRKPFSAFAQSKPGRDETPLTVALVGDCAYAHDAPAKA
                     MLDAAIGLKRAGARPVVFAGLGPPDPRLAAAGVETVCLDATDPPSRTSKAAAMMQGFW
                     SFRAAKALSEFLRTLPDENAVLHLHGYAQALSPSIKTAIAARGLPAIFTMHDYFLLCP
                     TGGLYNYQTREICGLAPLSRACVSTHCDMRSYGRKLWRVGRHLLAESGPALSSLFSDI
                     ILATRFQREAVGALLPKARQHILSNPIDVPDFGPRRSTGEGFVFLGGLSPEKGPLVFA
                     EAAKRAGVTATFIGDGPLRGEIAARYPDARLVGWKNPEVAQAIMRDARALVFPSLWYE
                     AQGSAAIEAKALGVPVIVSDACAARDEIERGKAGLLFRSDDAASLAEALVRLGDEDYA
                     AALSRGAYQSYWAAPRSLQRHVDGLLRIYRGMVRNRRAVAGT"
     gene            4681..4821
                     /locus_tag="VKU03_05520"
     CDS             4681..4821
                     /locus_tag="VKU03_05520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70755.1"
                     /translation="MAATLGAISFSMLARIGESPSEFRFFATKNAEEAAASPETAAHL
                     AE"
     gene            4879..6939
                     /gene="parE"
                     /locus_tag="VKU03_05525"
     CDS             4879..6939
                     /gene="parE"
                     /locus_tag="VKU03_05525"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009340 - DNA topoisomerase IV complex
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IV subunit B"
                     /protein_id="HLJ70756.1"
                     /translation="MSMDNDLFGSAGPRRAAGGAKASRAPLPPKGKTADQRTPGEAGY
                     TAASIEVLEGLEPVRRRPGMYIGGTDAAALHHLFAEVIDNAMDEAVAGHATFIEVAME
                     DGGWIAVTDNGRGIPIDPHPKFPKKSALEVIMTTLHAGGKFDSGAYQTSGGLHGVGVS
                     VVNALSSRLEVEVARNQTLYRQAFERGHAVSKIETVGKAPNRRGTKVRFKPDEQIFGK
                     SAHFDPARIFKMARSKAYLFGGVEIRWRCAPSLLGPDSKVPAEAEFHFPGGLKDYLAA
                     DIEGQDLVVDQVFFGKVEKPGGHGSLEWAIVWLAGEDGFVHSYCNTIPTPDGGTHEAG
                     LRAALLRALKDHAERAGQSKRASALTSDDVMATCAALISVFIREPEFQGQNKGRLQTA
                     EAARIVESTIRDAFDHWLAAAPSQANKLLDWSIERAEERLRRKAEKDVARKTAVRKLR
                     LPGKLADCTNTSSQGSELFIVEGDSAGGSAKQARDRASQAVLPLRGKILNVASATRDK
                     LAQNQQLADLLQALGCGSGSHYRAADLRYDKIIVMTDADVDGAHIASLLITFFYRQTP
                     KLIEEGHLYLAVPPLYRLTQGAKSLYARDDAHKEELLKTGFSGRGAIDISRFKGLGEM
                     PTKDLRETTMDPARRTLLRVSIADAERQATADTVERLMGSKPEARFAFIQERAPRAIE
                     LVDI"
     gene            7015..7290
                     /locus_tag="VKU03_05530"
     CDS             7015..7290
                     /locus_tag="VKU03_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70757.1"
                     /translation="MAWRFATDQRASTVEKFAVAAAAIGLLAFLCGTSVEQLASAGRL
                     PTVAFLQPDQYVATRDKPNETGAIDYMATGSINGRIVLDPCTGRRKP"
     gene            7332..8228
                     /locus_tag="VKU03_05535"
     CDS             7332..8228
                     /locus_tag="VKU03_05535"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ70758.1"
                     /translation="MADASLESIGAPTRGVDRIGWAALLVGTAIIAWSGIIARFLDVG
                     PVAGAAWRMGLAVPALAVWSSLASGRRLSLRRAAPFAGPLVLAGLAFAVDVGSFHIAL
                     TGTKVANATFIGNVAPILAVVGGALFFGEHPPGRVWLALALALLGSWVMAGMSAPAAL
                     GYGDLFAFAAANAYATYLLVIKRLRLGLDGPTATLWSAAVSALALALAAWLKGEVLIP
                     HSLAGWTTVILLGIVSHALGQGLTSVAVGRLSVGVVALVILAQPPFSALIAFVVLGEA
                     MTPVQIVGGAMILIATAAARPR"
     gene            8713..9369
                     /locus_tag="VKU03_05540"
     CDS             8713..9369
                     /locus_tag="VKU03_05540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HLJ70759.1"
                     /translation="MSKGKDFRGPRKRGFDDDGPMPYDPRPRAPRSFDSGGFGGPQPM
                     SPAPASGPSVDAVVKWFKADKGYGFVELANGQGDAFLHVNALHSSGFDTVPPGAKLKV
                     MVGAGAKGAQVTRVLEVDTAGAAERAPPPRTFGDAPRPRRAPPDPSTAVSVAGKVKWF
                     DDAKGFGFVASDDGGKDVFVHISILGPSGITHLAEGQPVNMRVVDTPKGREAISISPA
                     "
     gene            9450..10304
                     /gene="mazG"
                     /locus_tag="VKU03_05545"
     CDS             9450..10304
                     /gene="mazG"
                     /locus_tag="VKU03_05545"
                     /EC_number="3.6.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside triphosphate pyrophosphohydrolase"
                     /protein_id="HLJ70760.1"
                     /translation="MTPSRDIDRLLEIMAALRTPGSGCPWDLEQDFSTIAPYTIEEAY
                     EVADAIERGDFVDLRDELGDLLLQVVFHARMAEEAKLFDFGGVVEAITTKLIRRHPHV
                     FGEARDLSPEAVKSLWAEIKAEEKRERAAAHERSGAPPTANGALSGVPLPLPALSRAL
                     KLQQKASRVGFDWNDAKAVLAKLREETAEIEEALAAGDEDAVKDEIGDLLFVVANLAR
                     HVQVDPEQALRGANAKFERRFAHIEARLKDEGRTPEQASLDEMDALWDEAKARETRPA
                     PRRPVQKP"
     gene            complement(10338..10913)
                     /locus_tag="VKU03_05550"
     CDS             complement(10338..10913)
                     /locus_tag="VKU03_05550"
                     /inference="COORDINATES: protein motif:HMM:NF028169.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ70761.1"
                     /translation="MLAAWEKAEKRRPGRPRSELSKNAILDVAYWQVMERGYPRVTAD
                     SLAKAAGAGKQTLYRWWPSKAAVILDALAEKGRARIDRPQEAAIRSGDLLGFLRAVIP
                     AMAAVGPVLRHLMAEAQFDPKLRALLRERLIEPRREPLRQILATRVADAHKREALVSA
                     IYGAIWYRLLLDEPVDEALALELAALAPPAR"
BASE COUNT         1862 a         3639 c         3673 g         1883 t
ORIGIN      
        1 cgacgagcat gaggaaggcc cgtccgcgat gctgcgcaag cgcgaagact ccgatcgcga
       61 cgatcgcggc gaccgccgcg gcggccgccc ggaccgcggc gaccgcgacc gcggcgatcg
      121 tccccggcgt ccgcgcgaag acgcccagcc cggagctctt taaatgtcga ccgcccctcg
      181 ccgtcccttt ttccgtcgcc gcaaaacctg ccccttttcc gggccgaacg cgccgaagat
      241 cgactacaag gacacgcgtc ttctgtcgcg ctacatttcc gagcgcggca agatcgtgcc
      301 gtcgcgcatc accgcggtca gcgccaagaa gcagcgcgag ctcgccgccg cgatcaagcg
      361 ggcgcgcttt ctcggcctgc tgccttacgt gatccgctaa ggcttgaagc cgcgaagcgc
      421 gccgaagcgc gcttcgtctg aaacaatgtc gaacgcgccg gatcagccgg cgcgtgatgg
      481 ctgggcgaag cgacgacctc gccctaaccg ccgtgaaagg cgcgggacag ctcgacgatg
      541 gttcaacgca tcgcaatcgc ccttggcgcg ggaatggtcg cggcgctgct tttcgccgtc
      601 atggcgaagg gcacgccggt cgcgatggcc ctcgcctatc tcgcgccgtt gccgatcgtc
      661 atcgccgcgt tcaactgggg accggatatg ggcgcggtcg cggcggtcac cgcctgcggc
      721 ggcgccgccg ctctggtcga cccctattcg ggcgccttgt tcggcgcggc gatcgccctg
      781 cccgcctggc tgctcagcgc gctttcttcg gcgcatgtcg gaattttccg ccgcgccgcc
      841 aaggaccagg agcagacggc cgtctggtgt ccgatcggcc tcgtcgtcac cgcggccgcc
      901 gtgatcggcg ccctgatcgg gctcagcgct ctgatctcgc tgatcgtgct ctacggcggc
      961 taccagaaag gcgtcgagga gctcgttcgg caattcaccg ccgatattcg cgacgccctc
     1021 gaccagatga tcgccctccc tgtcggcatg accgttgagg aatttgcgac gctggtggtc
     1081 aggatgtcgc cgcctatgct cgccgcggcg atcttcctga tgctctgcgc caatctctat
     1141 gtcggcgccc gcgtcgcgca aatttcccaa acgttgaagc ggccctggcc gaacctgccg
     1201 gaagcgctgg tcttgccgca gccgctcggc gtcgcgctgg ttctttgcct cggcctagcg
     1261 ctcacgctcg gcgatccgct gcgccaggcg gcgtggatcg gcgtcggcgc cttcgccgcg
     1321 gcctacgccc tgcaaggctt ggcgctcgcc catgggctaa cgcgcggcct gcagcatcgc
     1381 aatcccctgc tcttcgcgct ctatctggcc gtggcttttg cgccgcgctg ggtcgtgccg
     1441 gcggtgacca tggccgggct tctggagagc tttcttgcgc tgcgcgccaa acgcgtcgcg
     1501 gcggccaacg ccaacaacct caatcaacgc aagccttaag aaggagatcg tcatggaagt
     1561 gattttgctc gagcgcatcg cgcgcctcgg ccaaatgggc gacaccgtgc gggtcaagga
     1621 cggcttcgcc cgcaatttcc tgctgccgcg cggcaaggcg ttgcgcgcca ccgaggccaa
     1681 tcggaagaaa ttcgagggcc agcgccagca gctcgaagcg cgcaatctcg aattgaagcg
     1741 cgaggccaac gacgtctccg tcaagctgga cggccagagc ttcgtcatca tccggcaggc
     1801 cggcgagacg ggacagcttt acggctcggt ctcgagccgc gacatcgccg aggcggcgac
     1861 ggcggcgggc ttcacgatca accgcaacca ggtcgtcctg ctccatccga tcaagacgct
     1921 cggcctgcac aagacgccga ttcaattgca tccggaagtg accgcgaccg tgacgatcaa
     1981 tgtcgcgcgc tcgcaggagc aggccgagcg ccaggcgaag ggcgaagatc tcactgtgcg
     2041 cgaggagacc aatctcgacg atctcggcct ggaagtcggc gcggcgctcg ctgaagccgc
     2101 gccgccgcct cagggttgag cgcggctcct cgcctcttcc gctccgtcat tgcgacgggc
     2161 gcagcgccga agcaatccgt tcaggggctg taccctacga gcggatcgct tcgctcgcag
     2221 tgacggcgcc tggttcagta ggaaaagcgg aacgcgctga cgtgccggta ttcctcgtcc
     2281 ggccgcagca cgcaatcggg gaaatgccgg cggttcggcg aatccggata gacctgcggt
     2341 tcgaggcaga ggccggcatg cgggccgtaa tgcgcgccgt caaggccggg aaccgggcag
     2401 ttcagcttgg ccgcgtcata gaattgcacg ccgggctcgg tcgtgaagag gtcgagcgtc
     2461 aacccgtttt tcggcgagcg gagccgcgcc gccggcgcga ggccgtcggc gaggatgcgc
     2521 gcggcgagca cgaaattcgt atcgtaggtt tggccggacg cgtggcgaac cggccgcgcg
     2581 ctgcggaaat cgtagggcgt gccggcgact gcgcgaatct cgccggtcgg aatgagatcg
     2641 gcgtcggtcg gcgtgtaaaa atctgcgttc agcgtcagct cgtgatcgag aatgtcggct
     2701 gagccgtcca gattgaaata ggcgtgctgg gtcaggttga cgatcgtcgg acgatcgctg
     2761 accgcggtca attcgacgcg cagcgtcgag ggctcctcga tccggtagac gcatgtcgcc
     2821 tcgagcgcgc cgggaaagcc gctatcgcca tccgccgaat ggagcgtcag cgtcaccgag
     2881 gtttcgtcat gattggcgag ccgccacggc cgcttgccga agccgtgcgg gccgccatgc
     2941 aatgtatgtt tctcgccggg cttgcggtcg agctcgtagt cgacgccgtc gagcgagaat
     3001 cggccattgg cgatgcggtt ggcgaaccgg ccgggaaccg cgccgaaact cggcgaatgg
     3061 gcctcgtaat cggcaagcga attcaagccg agggtcacgc gttgcgggcc gtttttcgac
     3121 ggcacgatga gatcgcggac gacggcgccc caattgagga ttttggcgac tgcgccggct
     3181 ttcgaagcga tcgtgatctc gacgatcgga acgccttccg cctcgccgaa cgcccggatg
     3241 ttcatcgccg ctccttcgct ttgaatgtcg agcgaagatg gcctaggaac gcccctaggt
     3301 caaagcgcgc cgcgtttcgc cgttaaaggc ctgcctcgcc ttcatgtccc ggctacggcc
     3361 cgcctgtttc gaaccattcc acgataaatg cgcagcaatc cgtcgacatg gcgctgcaga
     3421 ctgcgcggcg ccgcccaata ggattggtac gcgccgcgcg aaagcgcggc ggcgtagtct
     3481 tcgtcgccga gccggaccag cgcctcagcg agcgacgccg catcgtcgct cctgaacagc
     3541 aatcccgcct tcccccgctc gatctcgtcg cgggcggcgc aggcgtcgga gacgatcacc
     3601 ggcacgccga gcgccttggc ctcgatcgcc gccgagcctt gcgcttcgta ccagagggaa
     3661 ggaaagacca aggcgcgcgc atcgcgcatg atcgcctggg cgacttccgg gtttttccag
     3721 ccgaccagcc tggcgtccgg atatctggcg gcgatctcgc cgcgcaaggg cccgtcgccg
     3781 ataaaggtcg cggtcacgcc ggcgcgtttg gccgcctcgg cgaagaccag cggccccttc
     3841 tccggcgaca gtccgcccaa aaatacgaag ccctcgccgg tcgagcggcg cggcccgaaa
     3901 tcgggaacgt cgatcggatt ggacaggata tgctggcgcg ccttcggcag cagcgcgccg
     3961 accgcttcgc gctgaaatcg cgtcgccagg atgatgtcgg agaacaggct cgacagagcc
     4021 gggccggatt cggcgagaag atgccggccg acccgccaca gcttgcggcc gtaggagcgc
     4081 atgtcgcaat gggtcgaaac gcaggcgcgc gacagcggcg cgaggccgca aatctcccgg
     4141 gtctggtaat tgtacaagcc gccggtcggg cagaggagaa aatagtcgtg catcgtgaag
     4201 atcgccggca ggccgcgggc ggcgatcgcc gtcttgatcg aaggcgacag cgcctgggcg
     4261 tagccatgca gatgcagaac ggcgttttcg tccggcagcg tgcggaggaa ttcgctcaac
     4321 gccttggctg cgcgaaagct ccaaaagccc tgcatcatcg cggcggcctt ggatgtccgg
     4381 cttggcggat cggttgcgtc gaggcaaacc gtctcgacgc cggcggcggc gaggcgcgga
     4441 tcgggcggcc cgagccccgc gaagacgaca ggccgcgcgc cggcgcgttt caatccgatg
     4501 gcggcgtcga gcatcgcctt ggccggggcg tcatgggcgt aggcgcagtc gccgaccagc
     4561 gcgacggtga gcggcgtctc gtcgcgaccg ggcttgcttt gagcgaatgc ggagaagggc
     4621 ttccgaagcg cggcgggaga cgccatatgg aaattccaaa atgaaaatga ggggacggct
     4681 atggcggcga cgctaggcgc catttctttc tcaatgcttg cgcggatcgg cgaatctccg
     4741 agcgaattcc gttttttcgc cacgaagaac gccgaagagg cggcggcttc gccggaaacc
     4801 gccgctcatc tcgccgagtg atccgtgtta gggtcgcggg ctcgattcgc gtcccctctt
     4861 cagagaagat gattaaagat ttcaatggat aatgatcttt tcggctctgc cggcccgcgc
     4921 cgcgccgccg gcggcgcgaa agcgtcgcgc gcgccgcttc cgccgaaggg caaaaccgcc
     4981 gatcagcgca cgccgggcga ggccggctat accgccgcct cgatcgaagt cttggaaggc
     5041 ctggagccgg tcaggcggcg gccgggcatg tatatcggcg gcacggacgc cgcggcgctg
     5101 catcacctct tcgccgaagt catcgacaat gcgatggacg aggcggtggc cggccatgcg
     5161 accttcatcg aagtcgcgat ggaggatggc ggctggatcg cggtcaccga caatggccgc
     5221 ggcattccga tcgacccgca tccgaaattt ccgaagaaat ccgcgctcga agtgatcatg
     5281 acgacgctgc atgccggcgg caagttcgac tccggcgcct atcaaacctc aggcggtttg
     5341 cacggcgtcg gcgtttctgt cgtgaatgcg ctgtcgtcgc ggcttgaagt cgaagtcgcc
     5401 cgcaatcaga cgctctatcg ccaggcgttc gagcgcggcc acgcggtcag caaaatcgag
     5461 acggttggca aggcgcccaa ccggcgcggc accaaggtgc gcttcaagcc cgacgagcag
     5521 attttcggca aaagcgcgca tttcgatccg gctcgcatct tcaagatggc gcgctcgaaa
     5581 gcctatctgt tcggcggcgt cgagattcgc tggcgctgcg cgccaagcct gctcgggccc
     5641 gacagcaagg tcccggccga agcggagttt catttccccg gcggcctgaa agactatctg
     5701 gccgccgata tcgagggcca ggacctggtc gtcgatcagg tcttcttcgg caaggtcgag
     5761 aagcccggcg ggcatggctc gctcgaatgg gcaattgttt ggctcgccgg cgaggacggc
     5821 ttcgtccatt cctattgcaa cacgattccg acgccggacg gcggcacgca cgaagcgggc
     5881 ttgagagcgg cgttgctgag ggcgctcaag gatcacgccg aacgcgccgg ccagagcaag
     5941 cgcgcctcgg ccctgaccag cgacgacgtc atggcgacct gcgccgccct gatttcggtc
     6001 ttcattcgcg agccggaatt tcagggccag aacaagggcc gcttgcaaac cgcggaggcc
     6061 gcaaggattg tcgaatcgac gatccgcgac gctttcgacc actggctcgc cgccgccccc
     6121 tcccaggcca acaagctgct cgactggtcg atcgagcgcg ccgaagagcg tctgcgccgc
     6181 aaagccgaga aggacgtcgc ccgcaagacc gccgttcgca agctgcgcct gcccggcaag
     6241 ctcgcagact gcacgaacac gtcgtcgcaa ggctctgaac tctttattgt agagggcgat
     6301 tcggccggcg gctccgcgaa gcaggcgcgc gatcgcgctt cgcaggccgt gctccccttg
     6361 cgcggcaaaa tcctgaacgt cgcctcggcg acgcgcgaca agctcgccca gaatcagcag
     6421 ctcgccgatt tgctccaggc gctcggctgc ggctcaggct cgcattatcg cgccgccgat
     6481 ctgcgttacg acaagatcat cgtcatgacc gacgccgatg tcgacggcgc gcatatcgct
     6541 tcgctgctga tcaccttctt ttatcgtcag acgccgaagc tgatcgagga agggcatctg
     6601 taccttgccg tcccgccgct ctatcggctg acgcaaggcg cgaaatctct ctatgcgcgc
     6661 gacgacgctc acaaggagga actgctaaag accggctttt ccggccgcgg cgccatcgac
     6721 atcagccggt tcaaggggct cggcgaaatg ccgacgaagg atttgcgcga gacgacgatg
     6781 gacccggcgc ggcgcacttt gttgcgcgtg tcgatcgccg acgccgagcg ccaggcgacg
     6841 gccgacacgg tcgagcggct gatgggctcc aagccggagg cgcgcttcgc cttcatccag
     6901 gagcgggccc cgcgcgccat cgaactcgtc gacatttaat cgcgccgtca aggcttcatt
     6961 taccatgttc gtcctaatgt gcccggcgat tggacagagg gccgaggggc gaagatggcc
     7021 tggcgttttg cgacggatca gcgcgcgtcg acggtcgaga aattcgccgt ggcggcggcc
     7081 gcaattgggc tcctcgcctt tctatgcgga accagcgtcg agcaattggc gagcgccggg
     7141 cgactgccga ccgtcgcctt tcttcagccc gaccaatatg tcgccacgcg cgacaagccg
     7201 aacgagaccg gcgcgatcga ttacatggcg acgggctcga tcaacggacg catcgtgctc
     7261 gatccctgca caggccggcg caaaccctga tcgtttctca cgcctgagaa acgcgccgcc
     7321 ggcgcggcgc gatggctgac gcgtctctcg aatcgattgg cgcgccgacg cgcggcgtcg
     7381 accgcatcgg atgggccgcg cttcttgtcg gcacggcgat catcgcctgg tccggaatta
     7441 tcgcgcgctt tctcgatgtc ggccccgtcg ccggcgccgc ttggcgcatg ggcttggcgg
     7501 tgccggcttt ggcggtctgg tcgagcctcg cctcgggacg gcgactttcg cttcgccggg
     7561 ccgcgccttt cgccgggccg cttgtcttgg ccggtctcgc ctttgcggtc gatgtcggca
     7621 gtttccatat cgcgctgacc ggcaccaagg tcgccaacgc gaccttcatc ggcaatgtcg
     7681 cgccgatcct ggcggtggtc ggcggcgcgc tgttcttcgg cgagcatccg ccgggtcgcg
     7741 tctggctcgc gctggctttg gcgcttctcg gcagttgggt tatggccggg atgagcgcgc
     7801 cggcggcgct cggctatggc gatctcttcg ctttcgccgc ggccaacgcc tacgcgactt
     7861 accttctcgt catcaagcgc ttgcgcttgg gcctggacgg cccgacggcg actttatggt
     7921 ccgccgcggt ctcggccttg gcgctcgccc tcgcggcttg gctcaaaggc gaagtcctga
     7981 tcccgcacag tcttgccggc tggacgacgg tgatcctgct cggaatcgtc tcgcacgcgc
     8041 ttggccaggg cttgacctct gtcgccgtcg gccgcctatc ggtcggcgtg gtcgccctgg
     8101 ttattctcgc ccagccgcca ttttccgcgc tgatcgcctt cgtcgtcctt ggcgaagcga
     8161 tgacgcccgt gcaaatcgtc ggcggcgcga tgattctcat cgccacggcg gcggcgagac
     8221 cgcgttagta tacgaacaaa taccctagaa gttctaatgc tgttggacaa acgacacgaa
     8281 tcatctggct ctcgttttag aaatgcgata aagtggcctt gcgtcgacca gcctgaacca
     8341 tgctggacgg catcggcagg ctttattgat gcagtccttt cgtcctgttc tgtcccccgg
     8401 cccggagccg gcgcgcggcg gcccaggctt tctttttgga ttatcggttg ctcccgctct
     8461 cgaatggatt tgtccgacgg acggagcggc gcgcgccggc gagcgattga cgccgccgcg
     8521 cctctcaaat agcggagcaa gctttgcggc ttgaagcgcc gggtttccgc gctgttcaga
     8581 gccgacccac gccggatatg gcgtttcgca aaaggatcag gcaaaagacc ggcgagctgt
     8641 atttcggttc ggccggtccg gtgacggtag gcgtaggcag aatgcgtagt ttgtcggagc
     8701 gacaggggca acatgagcaa gggtaaggat ttccggggac cgcgaaagcg cgggttcgac
     8761 gatgacgggc cgatgcctta cgatccgcgg ccgcgcgccc cgcgcagttt cgatagcggc
     8821 ggctttggcg ggccgcagcc gatgtcgccg gcgccggcga gcgggccgtc ggtcgacgct
     8881 gtcgtcaaat ggtttaaggc cgacaaaggc tacggcttcg tcgaattggc caatggccag
     8941 ggcgacgcct tcctccatgt caacgcgctg cattcgtcgg gattcgacac ggtgccgccc
     9001 ggcgccaagc tcaaggtgat ggtcggcgcc ggggccaagg gcgcgcaggt gacgcgcgtg
     9061 ctggaggtcg acacggccgg cgccgccgag cgggcccccc cgccgcgcac ctttggcgat
     9121 gcgccgcggc cgcgccgggc gccgcccgat ccgtcgacgg cggtcagcgt cgccggcaag
     9181 gtcaaatggt tcgacgacgc caagggcttc ggcttcgtgg cgagcgatga cggcggcaag
     9241 gacgtgttcg tccatatctc gattttgggc ccgtccggca tcacccatct ggccgagggc
     9301 cagccggtca atatgcgcgt ggtcgacacg ccgaagggcc gcgaggcgat ctcgatctcc
     9361 ccggcgtaag ccgacaaatc gacgctgcaa agccaaggcg cgcggtttaa aagaccgcgc
     9421 gcctttttcg ttccttcaag agattcggga tgacgccttc ccgcgacatc gatcgcctgc
     9481 tcgaaatcat ggcggccttg cgcacgcccg gctcgggatg tccgtgggat ctcgagcagg
     9541 atttttcgac catcgcgccc tacacgatcg aggaggcgta cgaggtcgcc gacgcgatcg
     9601 agcgcggcga tttcgtcgac ctgcgcgacg agctcggcga cctgctgctg caagtcgtct
     9661 ttcacgcccg catggccgaa gaggccaagc tgttcgattt cggcggcgtc gtcgaggcga
     9721 tcacgacgaa gctgatccgc cgccatccgc atgtcttcgg cgaggcgcgg gacttgtcgc
     9781 cggaggcggt gaagagcctc tgggccgaga tcaaggccga ggaaaagcgc gagcgcgccg
     9841 cggcgcatga aaggtccggc gcgccgccga cggcgaacgg cgctctttcc ggcgtgccgc
     9901 tgcccctgcc cgctctgagc cgcgcgttaa agcttcagca aaaggcgagc agagtcggct
     9961 tcgactggaa cgacgcgaag gccgtgctcg ccaaattgcg cgaggagacg gccgagatcg
    10021 aagaggcgct cgccgccggc gacgaagacg cggtcaagga cgagatcggc gacctccttt
    10081 ttgtcgtcgc caatctcgcc cgccatgtgc aggtcgatcc cgaacaggct ttgcgcggcg
    10141 ccaacgccaa gttcgagcgc cgcttcgccc atatcgaggc gcggctcaag gatgagggcc
    10201 gcacgcccga gcaagcgagc ctcgacgaaa tggacgcgct ctgggacgag gcgaaagccc
    10261 gcgaaacacg tcccgcgcca aggagacccg tccaaaaacc gtgacgttct gcacgaggcg
    10321 aggcaaatag cctgaggtca tctcgccggc ggcgccaaag cggcgagctc gagtgcgagc
    10381 gcttcgtcga ccggctcgtc gagcagcagc cgataccaga tcgccccata gatcgccgag
    10441 accagggctt cccgcttatg cgcatccgcg acgcgcgtcg caaggatctg gcgcagcggc
    10501 tcgcggcgcg gctcgatcag gcgctcgcgc agcagggcgc gcagcttcgg atcgaattgc
    10561 gcctcggcca tgaggtggcg caagaccggc ccgaccgccg ccatggccgg gatcacggcg
    10621 cgcaggaagc cgagcaggtc gcctgaccgg atcgccgcct cctgcggccg atcgatgcgc
    10681 gcgcggccct tctcggccaa cgcgtcgaga atcacggccg ccttgctcgg ccaccagcga
    10741 tagagggtct gcttgccggc cccggcggcc ttggcgagcg aatcggccgt gacgcgggga
    10801 tagccgcgct ccatcacctg ccaataggcg acatcgagaa tggcgttctt gctcagttcg
    10861 gagcgcggcc gtccaggccg gcgtttttcg gccttttccc atgccgccag catagaacca
    10921 gattgcgaga cttcaagtct cgcaatataa tgagactccg agttccggaa ttggaggcga
    10981 cgatgaaagc ttggcgcgcg ctgggaaacg gccgattgga actgcaggac ctgccggcgc
    11041 cgcggccggc cgcgggc
//
LOCUS       DATGJW010000184         3729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_346012, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000184 DATGJW010000000
VERSION     DATGJW010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3729
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346012"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(276..1553)
                     /locus_tag="VKU03_05555"
     CDS             complement(276..1553)
                     /locus_tag="VKU03_05555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006727825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLJ70762.1"
                     /translation="MPKRAIPAPDRRKPPPAPNARSSKSPATPALHLHGRPTLRVLGT
                     EITLLEQVRERAEADLGMQIVFENLDFVSAQRKAACQPETYDVYDQCFHNLDIVWFWR
                     AIQPIDIARIALWDEVNDLTKTGRIHPGAAIGWGDAPVAKLFVQSNNSLGAAPSDRIS
                     MLPAVHNFDSFAYNSALVDGHLEPSASWGVLFDDRWAGRVALVDEPAIGVFDAALAAQ
                     ARGEAHFEDIGNMSTAEIDRLIDVLMEKKRAGYFCGFWKTTPEAADMMLHGRAAIQSM
                     WSPGMSLLRQRGFPAREATPQEGYRAWHGGLCLAAHLSGRALDVAYEYLNWWLDGWAG
                     AVMARQGYYMSAPERVRRHLSEAEWNYWYEGEAAAADLPGPSGDISIRRGEIRSGGAY
                     RQRASRIAVWNTTMDEHNYLVRRWAKLVGPPDR"
     gene            complement(1742..2752)
                     /locus_tag="VKU03_05560"
     CDS             complement(1742..2752)
                     /locus_tag="VKU03_05560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLJ70763.1"
                     /translation="MSEAVPDLTPVALLTGFLGSGKTTLLRRLLADPSLRDTAVIINE
                     FGEIGIDHHLVEKLDDQMVLLSSGCLCCTVRGELAATLRDLHSRRARGLAPPFRRVAI
                     ESTGLADPFPVLSTLSADPVLRHHFTAAGVATTVDAVNGLAQLGAHVESVRQAAAADV
                     IVLTKTDLAAPAAASRLVRKLRAINPIAPLLRGAEEPLDPNAILSPRRSGFRAMAEPA
                     AEAAHSAVQAFSIVVDEPIAWAAFGVWLTMLLHRHGDRILRVKGILALQGEERPVAVH
                     GVQRLVHAPTHLDRWPDGDRRSRLVFIVEGLDPALIRRSFAVFNRLSRSAKSRSFDVT
                     YS"
     gene            complement(2745..3074)
                     /locus_tag="VKU03_05565"
     CDS             complement(2745..3074)
                     /locus_tag="VKU03_05565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70764.1"
                     /translation="MCSLCGVLGGVDHWADAAARDGVYTRNADPTRRRRERAERVRIA
                     NRALAHYGMRLADWQGASYLLSTFTGKSEMVEDFGHLWPAAEKLSGRLCDPLAADLLA
                     RLEAGRE"
     gene            complement(3092..>3729)
                     /locus_tag="VKU03_05570"
     CDS             complement(3092..>3729)
                     /locus_tag="VKU03_05570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="polysaccharide deacetylase"
                     /protein_id="HLJ70765.1"
                     /translation="IGIHGYSHENPIAMTPEQEEEVLDKCIGLVAKLAGKRPTGYVAP
                     WWEFSRVTNELLLKKGIKYDHSLMHKDFQPYYVRVGDSWTKIDYSKKPSEWMIPLKRG
                     HQTDLIEIPASWHLDDLPPMMFIKKSPNSHGFVSPRHLEEMWRDQFDWVYREHDYAVF
                     PITIHPDVAGRPQVLLMLERLYNHMVRHPGVRFVTMNEMADDFAKRSPRRK"
BASE COUNT          674 a         1218 c         1244 g          593 t
ORIGIN      
        1 ttcgaccgga aaagccagcg ggcgccattt tgcaaccccg aagcccagcg ccgcagccga
       61 cgcccaggcc gctgcatcga ataaagagtg gatgatcgtt ggcgtcatga agcggcagtg
      121 gacagacaat aggcgccggg agcctcgcgg gaactggaac aggaacaaga accggggtca
      181 agaggcgatc aggcgtctcc tcggccggcg cttcttgatc acgattgcga caaggcccac
      241 ttcgatccgt cgacgggaga cctcctcgcg cccgctcacc ggtccggcgg gcccaccaac
      301 ttggcccagc gcctgacgag gtaattgtgc tcgtccatgg tcgtgttcca taccgcgatg
      361 cggctggcgc gctggcgata ggcgccgccg ctgcggatct cgccgcggcg gatcgaaatg
      421 tcgccgcttg ggcccggtag gtcggcggcg gcggcctcgc cctcgtacca atagttccac
      481 tccgcctcgg agagatggcg gcgcacccgc tcaggcgccg acatgtaata gccctgccgc
      541 gccatcaccg cgccggccca gccgtcgagc caccaattga gatattcata ggcgacgtcg
      601 agcgcccgcc cgctcagatg cgccgccagg cacaatccgc cgtgccaggc gcggtagccc
      661 tcctgcggcg tcgcctcgcg cgccggaaag ccgcgctggc gtaaaagact catgccgggc
      721 gaccacatgc tctgaatggc ggcgcggccg tggagcatca tgtcggcggc ttccggcgtc
      781 gtcttccaga agccgcaaaa atagcccgcc cgcttcttct ccatcagcac gtcgatcagg
      841 cggtcgattt ccgccgtcga catgttgccg atatcctcga aatgcgcttc gccgcgcgcc
      901 tgcgcggcga gcgcggcgtc gaacacgccg atcgccggct cgtcgacaag cgcgacccgg
      961 cccgcccagc gatcgtcgaa caggacgccc caagaggcgc tcggctcgag atggccgtcg
     1021 accagggcgg aattgtaagc gaaggaatcg aaattatgca cagccggcag catgctgatc
     1081 cggtcagatg gcgcggcgcc gagcgaattg ttcgactgca caaacaactt ggcgaccggc
     1141 gcatcgcccc agccgatcgc cgcgccggga tggattcggc ccgtcttggt caggtcgttg
     1201 acctcgtccc aaagcgcgat ccgcgcgatg tcgatcggct gaatggcgcg ccagaaccag
     1261 acgatgtcga gattgtggaa acactgatcg tagacgtcgt acgtctccgg ctggcacgcc
     1321 gccttgcgct gggcgctgac gaaatcgaga ttctcgaaga cgatctgcat gccgagatcg
     1381 gcttcggcgc gctcccgcac ctgttcgagc agcgtgatct cggtgccgag cacgcgcagc
     1441 gtcggccgcc cgtggagatg aagcgccggc gttgcaggcg actttgacga tcgggcgttc
     1501 ggcgcgggcg gcggcttgcg gcgatccggc gcggggatgg cgcgcttagg catgttcttt
     1561 ttcattgatc aacaagcgct gaggctcatc ctcgcccgag ccctctcccg caagcgggag
     1621 aggggggcga agcggctgtc atcggcgctg taagacaaag acgaagcgcc gagcacctcc
     1681 cttctcccgc ttgcgggaga aggtcgccgc gcagcggccg gatgagggta aaccgcgatc
     1741 gtcatgagta cgtcacgtca aaggatcggc tcttggcgga gcgcgagagc cggttgaaga
     1801 ccgcgaagga gcggcgaatc aaggccgggt caagcccctc gacgatgaag acgagccgcg
     1861 accttcggtc gccgtccggc caacgatcga gatgggtcgg cgcgtgaacg aggcgctgaa
     1921 cgccgtgaac cgcgaccggc cgctcctcgc cctgcaaggc gaggattccc ttgacgcgca
     1981 agatgcggtc gccatgccga tggaggagca tggtcagcca gacgccgaag gccgcccagg
     2041 cgatgggctc atcgacgacg atcgagaaag cctggacggc ggaatgggcg gcctcggccg
     2101 ccggctcggc catggcgcgg aagccgcttc gccggggcga caggatggcg ttcggatcga
     2161 gcggctcctc cgcgccgcga agcagcggcg cgatgggatt gatcgcccgc agcttgcgga
     2221 ccaatcggga cgctgccgcc ggcgcggcga ggtcggtctt ggtcaggacg atcacgtcgg
     2281 ccgccgccgc ctgccgcacc gattcgacat gcgctccaag ctgcgcgagc ccgttcaccg
     2341 cgtcgaccgt cgtcgcgacg cccgcggccg tgaaatgatg ccgcaggacc gggtccgcgc
     2401 tcagcgtcga gaggacggga aagggatcgg cgaggccggt gctctcgatc gccacgcgcc
     2461 ggaacggcgg cgccaggccg cgcgcgcggc gcgaatggag atcgcgcaag gtcgcggcga
     2521 gttcgccgcg caccgtgcag cacaagcatc ccgagctcag cagaaccatc tggtcgtcga
     2581 gcttctcgac gagatgatga tcgatgccga tctcgccgaa ctcattgatg atgacggcgg
     2641 tgtcgcgcaa agagggatcg gcgagcaggc ggcgcagcag cgtcgtcttg cccgagccga
     2701 ggaatccggt cagcagcgcg accggcgtca ggtccgggac ggcctcactc acgcccggcc
     2761 tcgagccgag cgaggagatc ggcggcgagc ggatcgcata ggcggccgga gagtttttcc
     2821 gccgccggcc agagatggcc gaaatcctcg accatttcgc tcttgccggt gaaggtcgag
     2881 agtaggtacg acgcgccctg ccaatcggcg aggcgcatgc cgtaatgcgc cagcgctcga
     2941 ttggcgatcc gtacccgctc ggcgcgctcg cggcggcggc gcgtcggatc ggcgttgcgc
     3001 gtatagacgc cgtcgcgcgc cgccgcatcc gcccagtgat cgacgccgcc aagcacgccg
     3061 caaagcgagc acatcggcgc cccctttccc gctatttccg gcgcggcgaa cgcttggcga
     3121 aatcgtccgc catctcgttc atcgtcacga agcgcacgcc gggatgacgg accatatgat
     3181 tatagagccg ctcgagcatg agaaggacct gcggccgccc ggcgacatcg ggatgaatgg
     3241 taatcggaaa gaccgcgtag tcatgctcgc ggtagaccca gtcgaactga tcgcgccaca
     3301 tctcctccaa atgccgcgga ctgacgaagc cgtgactgtt cgggcttttc ttgatgaaca
     3361 tcatcggcgg cagatcgtcg agatgccagc ttgccggaat ctcgatgaga tcggtctggt
     3421 ggccgcgctt cagcgggatc atccattccg aaggcttctt cgaatagtcg atcttcgtcc
     3481 aggaatcgcc gacccgcacg taataaggct ggaaatcctt atgcatcagc gagtggtcgt
     3541 atttgatgcc cttcttgagc agcaattcgt tggtgacgcg ggagaactcc caccacggcg
     3601 cgacgtagcc ggtcggccgc ttgccggcca gcttggcgac gaggccgatg catttgtcga
     3661 gcacctcctc ctcttgctcg ggcgtcatcg cgatcggatt ttcatgcgaa tagccgtgga
     3721 tgccgatat
//
LOCUS       DATGJW010000185         7232 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1071172, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000185 DATGJW010000000
VERSION     DATGJW010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7232)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7232)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7232
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1071172"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VKU03_05575"
     CDS             <1..270
                     /locus_tag="VKU03_05575"
                     /inference="COORDINATES: protein motif:HMM:NF019303.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLJ70766.1"
                     /translation="VKGLIALADDPGKPLLIQGAQHVFSPARRLPAWPDADRERRIVV
                     IGEGLQPGEIERVWAVFMGAPAIDQPDRAALLDNPLATKLGGLLA"
     gene            complement(391..651)
                     /locus_tag="VKU03_05580"
     CDS             complement(391..651)
                     /locus_tag="VKU03_05580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase TusA family protein"
                     /protein_id="HLJ70767.1"
                     /translation="MPPAQSETDAGFDAAATLDALGLKCPLPALKTRRALAKLDAGAT
                     LIVLADDPMAAIDIPHLLHELGDELVAASEAGERLRFVIRKG"
     gene            767..1897
                     /locus_tag="VKU03_05585"
     CDS             767..1897
                     /locus_tag="VKU03_05585"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ70768.1"
                     /translation="MAGPYVECLDVLGAAAHADAWRALCGRALEANAFAEPGFLIAAA
                     RHIAPPGLEFVLVWESAERRRLLGVLALAPPRLGVAAVWRSKEAGLAALALDRDGAAA
                     AFEAAALWLSKMRPSVAGLQVPTLAAHGPTAALIEGFAGRRSLTFIAHNPRQRAVLYA
                     ARDGQKRLDAALPGKRLKEWRRLRRRFGERGGLVFRSAQGAEAAQAADAFLALEPRNW
                     KGRAGAPLNAEPGRAAFARSALAALAAEGKLRIDSLECAGAPAAMGLILLSQDRAFYW
                     KTAYDEAFAEFSPGVLLTLDISRKQQDDPRIAATDSCAIEGHPMIERLWPQRLELVDC
                     LVALRPGGAWRLKLWLAERALARHAKDAAKRLLFPLLGRKRS"
     gene            complement(1911..2132)
                     /locus_tag="VKU03_05590"
     CDS             complement(1911..2132)
                     /locus_tag="VKU03_05590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Trm112 family protein"
                     /protein_id="HLJ70769.1"
                     /translation="MDDDQSLPAPAEPREPTRIDPRLLEILVCPLTRSTLEYDAARQE
                     LISRAARLAYPIRDGIPIMLPEEARRIED"
     gene            2222..2779
                     /locus_tag="VKU03_05595"
     CDS             2222..2779
                     /locus_tag="VKU03_05595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70770.1"
                     /translation="MSVAPIEIHGYAIVTDDDRIAGADGATPSGLRNEADWLNFQREL
                     DQADLVVLGRRGHQANPNVKGRRRLVVSNSAARLEAREGEIWWRPAAAPWRDVAAALL
                     PKGGRVAIPGGQGVFDLFLAIGYDAFHLTRGLGVIAPGGRALFSACDQRASAEAVLTK
                     AGLQPREKRILDKAANIVLTVWRKR"
     gene            complement(2947..3321)
                     /locus_tag="VKU03_05600"
     CDS             complement(2947..3321)
                     /locus_tag="VKU03_05600"
                     /inference="COORDINATES: protein motif:HMM:NF021001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2019 domain-containing protein"
                     /protein_id="HLJ70771.1"
                     /translation="MSEVFFSAMSIDQLLKRFEEACLEQYDTYITDDLEKFETNFRIL
                     VSIKDELKARGPEARRSLLRLLSHDNPHVRLQAAKFVYPVARDEAKTSLQDIAAAKLP
                     DQSLDAKMTLHRLEEVPDCLDH"
     gene            complement(4064..5641)
                     /gene="serA"
                     /locus_tag="VKU03_05605"
     CDS             complement(4064..5641)
                     /gene="serA"
                     /locus_tag="VKU03_05605"
                     /EC_number="1.1.1.95"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004617 - phosphoglycerate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HLJ70772.1"
                     /translation="MPRVLISDKLSPAAVAIFKERGVDVDVKPGLDKEELAKIIGEYD
                     GLAIRSATKVTAKLLERAEKLKVVGRAGIGVDNVDIPAATARGVIVMNTPFGNSITTA
                     EHAIALMFALARQIPSADASTQAGKWEKNRFMGVEITGKVLGIIGCGNIGSIVATRAI
                     GLKMRVIAYDPFLNEERAAELGVEKVELDDLLKRADFITLHTPMTAQTKNILSAENIA
                     KTKKGVRIVNCARGGLVDEKALRAALDSGHVAGAAFDTFVEEPATSNPLFGHPNVVAT
                     PHLGASTTEAQENVALQVAEQMSDYLMRGAIANAVNFPSITAEEAPKLKPFIALAERL
                     GSFAGQLTDSPITKVTIVYEGAVASLKIKALTASAIAGLLRPLLADINVVSAPSVAAQ
                     RGVIIEEVTRAAQGDYESLITLTVTTPGMDRAVAGTVFHDGKPRILSIKDIKVDAEFA
                     PSMIYVTNEDKPGFIGRFASLLGEAGVNIATFALGRDQQGGSAIALVEIDGAPPNGLL
                     DKVGKIPGVKQAKALRF"
     gene            complement(5771..6937)
                     /locus_tag="VKU03_05610"
     CDS             complement(5771..6937)
                     /locus_tag="VKU03_05610"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine transaminase"
                     /protein_id="HLJ70773.1"
                     /translation="MANAPAARPNNARFSSGPCAKRPGWTPEILKDAALGRSHRAKLG
                     KAKLKLAIDLTRDVLQVPADYRIGITPGSDTGAVEMALWSLLGARGVDILAWESFGEG
                     WVTDVVKQLKLDARVLKASYGELPDLSQVNFDRDVVFTWNGTTSGVRVPNADWIKADR
                     KGLTICDATSAAFAQKIDWTKLDVVTFSWQKALGGEAAHGMLILSPRAVERLETYKPA
                     WPLPKVFRMTKGGKLIEGIFEGETINTPSMLCVEDYLDALQWAKRIGGLNALIARADA
                     NLKTIADWAAKTPWIDFLAADPATRSNTSVCLKVIDPAIAGLPAEAQAAFAKAIASLL
                     EREKVAYDIGAYRDAPPGLRIWCGATIEKSDVEALTHWLDFAFAEEKAKLAKAA"
     gene            complement(7125..7223)
                     /locus_tag="VKU03_05615"
     CDS             complement(7125..7223)
                     /locus_tag="VKU03_05615"
                     /inference="COORDINATES: protein motif:HMM:NF025742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnA antitoxin family protein"
                     /protein_id="HLJ70774.1"
                     /translation="MSRDVVEAFKAKGPGWQSRIDETLRASLRKTG"
BASE COUNT         1171 a         2329 c         2404 g         1328 t
ORIGIN      
        1 gtgaaaggct tgatcgcgct cgccgacgat cccggcaagc cgctgctgat tcaaggcgcg
       61 caacatgtct tcagccctgc ccgccgcctg ccggcctggc ccgacgccga ccgcgaaagg
      121 cgcattgtgg tgatcggcga aggcttgcag cccggcgaga tcgagcgggt ttgggcggtc
      181 tttatgggcg cgccggcgat cgatcagccg gatcgcgccg ccttgctcga caatcccctc
      241 gcgacgaagc ttggcgggct gctcgcgtaa tattctgtag ccgcgctcgg cgagcgcggc
      301 cgagtcgtcg cccgcgacgc gatcgccgac cgcgtctaca ggcggcgacg aggcccgccg
      361 ggcggccaca gcccgcgcgg cgcgacctcc tcagcccttc ctgatcacga accgcaaacg
      421 ctcgcccgcc tcgctcgcgg cgacgagctc gtcgccaagc tcgtggagca gatgcggaat
      481 gtcgatcgcc gccatcggat cgtccgcgag cacgatcagc gtcgcgccag cgtcgagctt
      541 ggccaaagcg cgccgcgttt tcagcgctgg cagcgggcat ttcagcccaa gcgcgtccag
      601 cgtcgcggcg gcgtcgaatc cggcgtctgt ttcggactgg gcgggcggca tggcgcgatg
      661 ggtccatcaa tcgccgcgcc cagtcaagaa agccttgagg aaaggcggct aggctccgct
      721 tgcccgccga gcgtcggctc gctgcgggcg gggagcaggc gcgccgatgg ccgggcctta
      781 cgtcgaatgc ctcgatgttc tcggcgccgc ggcgcatgcg gacgcctggc gcgcgctgtg
      841 cggccgcgcg cttgaggcca acgctttcgc cgaacccggc tttctgatcg cggccgcccg
      901 ccatatcgcg ccgccggggc tcgaattcgt gctggtctgg gagagcgccg agagacggcg
      961 cctgctcggc gtgctggcgc tggcgccgcc gcggcttggc gtcgcagcgg tctggcggag
     1021 caaggaagcc ggtctcgccg ctctggcgct cgaccgggac ggcgcggccg cggccttcga
     1081 agcggcggcg ctgtggctga gcaaaatgcg cccgtcggtc gccggcctgc aggttccgac
     1141 cctcgccgcg catggcccga cggcggcgct gatcgagggc ttcgccggcc ggcgaagctt
     1201 gacctttatc gcgcataatc ctcgccagcg ggcggtcctc tacgccgcgc gcgacggaca
     1261 aaagcgcctt gatgcggctt tgcccggcaa gcgtctgaaa gaatggcggc ggctgcgccg
     1321 ccgtttcggc gagcgcggcg gcctcgtctt tcgctcggcg cagggcgccg aagccgcgca
     1381 agcggcggac gcgtttctgg cgctcgagcc gagaaattgg aaaggccgcg ccggcgcccc
     1441 tttgaacgcc gagcccggcc gcgcggcttt cgcgcgctcg gctttggcgg cgctggcggc
     1501 ggaaggcaag ctgcggatcg attccctcga atgcgccggc gcgccggcgg cgatggggct
     1561 gatcctcctc tcccaggatc gcgccttcta ttggaagaca gcctatgacg aggccttcgc
     1621 tgaattctcg cccggcgtgc tgctgacgct cgacatttcg cgcaagcagc aggacgatcc
     1681 gcggatcgcg gcgaccgatt cctgcgcgat cgagggccac ccgatgatcg agcgcctgtg
     1741 gccgcaacgt ctggagttgg tcgactgcct cgtcgcgctg cggcccggcg gcgcctggcg
     1801 attgaagctg tggctggccg agcgcgcgct cgcccgccat gcgaaagacg cggccaagcg
     1861 attgctcttt ccgctgctcg gccgcaagcg ttcctgatca ggccgaggct tcaatcctcg
     1921 atccgccgcg cctcttccgg cagcatgatc gggatgccgt cgcggatcgg ataggcgagg
     1981 cgggcggcgc gggagatcag ctcctgacgc gccgcatcat attcgagcgt cgaacgggtc
     2041 agcgggcaga ccaggatttc caggagccgc gggtcgatgc gggtgggctc gcgcggctcg
     2101 gccggggcgg gcaaagattg atcgtcgtcc atggcgggtg gtccgaaaac gtggctttgg
     2161 acgcggaaat agcatatcgg cgggcgtcgc gcccggccga tcaagagcgg gaggccgttt
     2221 cgtgtctgtc gccccgatcg aaatccatgg ctacgccatc gtcaccgatg acgaccggat
     2281 cgccggcgcg gacggcgcga cgccgagcgg gctgcgcaac gaggcggatt ggctcaattt
     2341 ccagcgcgag ctcgaccagg ccgatctcgt cgtgctcggc cgccgcggcc atcaggccaa
     2401 tcccaacgtc aaagggcggc gccggctcgt cgtctcgaac agcgccgcca ggctcgaagc
     2461 gcgcgaaggc gaaatctggt ggcgcccggc cgccgcgccc tggcgcgatg tcgcggccgc
     2521 gctgcttccc aagggcggac gcgtcgcgat tccaggcggt caaggcgttt tcgatctgtt
     2581 tctcgcgatc ggctacgacg cgttccacct cactcgcggc cttggcgtga ttgcgcctgg
     2641 cggacgcgcc cttttctcag cctgcgatca gcgcgccagc gccgaggcgg tcctaacgaa
     2701 ggcgggtttg cagccgcgcg agaagcgcat cttggacaaa gccgcgaaca tcgtactcac
     2761 agtgtggcga aagcgctgaa gggagcacga tcggttgtcg aggagcgccc cagccgggca
     2821 gacgggcgcg agccgcgaga cgagcgggcg agtcaatcca ttccgttcga cgcctgctcg
     2881 cgaaatgctt gagacgcctc tcgttccctt aacggccatt tcggccgtcg caaaatggcc
     2941 gcggcatcaa tggtcaagac aatcgggaac ttcttcgaga cgatgcaacg tcatcttcgc
     3001 gtcaagggat tggtcgggca atttggccgc cgcgatatct tgcaaagacg tcttggcttc
     3061 gtctcgcgcg acaggataga caaatttggc ggcctgaagg cgcacatgag gattgtcatg
     3121 gctcagcaat cgcaagagag acctgcgcgc ctcgggacca cgcgccttga gttcgtcctt
     3181 tatcgacacg agtatccgga aattcgtttc gaatttttcg aggtcatccg ttatgtatgt
     3241 gtcatactgc tctaaacacg cctcctcaaa gcgtttgaga agttggtcga tcgacatcgc
     3301 ggagaaaaat acctcgctca ttttagtatc cctacgtcac gcaagccaat taatccggct
     3361 cgacggcgtt cttcccagct cttgtctttt agatattcgc gcggtgtcat gttaccaaat
     3421 tccgtccgag gtctctcgta ccaactgttt aactcccaat gctttaccgt cgggattcgc
     3481 acgagatttt cgggagcatc catcaacgct tcttcgctgc cgtcagggtt cgcggtcgcc
     3541 tgttcgacaa tgtgatggat ttcatagcct ggcttgggaa agttcgccag cccgtcgccg
     3601 acgagcaggt aggaacctga acgggcgata aaattcgcag gctttcaatt gaaccggccc
     3661 gccaacgggc ggggctcgcg ctcgcctcgc gcgttgatgt ccatctgcgg ccagcaaagc
     3721 gcgaggacgc tatttatcgc tttctccgcg acagaggatt ggccctggct ggcaggcgac
     3781 gccgggggtc gaacagtgag cgccgccgcc gtcgcgcaaa cagatcgcgc agccgtcggt
     3841 ccactcagcg cagcgcggcg ccgcgatcgc gtagccttgc agcgatatgt tctcggccga
     3901 ggccggcctg gaaggaggcg gccgcgtctc gtccccgctt gcggagacga tgaagccgcc
     3961 gagcagcgcg agagcgaccg aaacccgtaa ttggacgcgc gcttgcatca tgcgccgcca
     4021 tggcgcgccg acaaccgcgg cgcaattctc aggcgccgcc gccctaaaac cgcagcgcct
     4081 tcgcctgttt cacgcccggt atcttgccga ccttgtccaa caggccgttc ggcggcgcgc
     4141 cgtcgatttc gaccagcgcg atcgccgatc cgccttgctg gtcgcggcca agcgcgaagg
     4201 tcgcgatgtt gacgcccgct tcgccgagca ggctggcgaa acggccgatg aagccgggct
     4261 tgtcctcatt ggtgacatag atcatcgacg gcgcgaattc ggcgtcgact ttgatgtcct
     4321 tgatcgacag gatgcgcggt ttgccgtcat gaaacacggt tccggcgacg gcccggtcca
     4381 tccccggcgt cgtcacggtc agcgtgatca ggctttcgta atcgccctga gcggcccgcg
     4441 tcacctcctc gatgatcacg ccacgctgcg ccgcaaccga cggcgccgag acgacattga
     4501 tgtcggccag cagcggccgc agcaggccgg cgatcgccga cgcggtcagc gccttgattt
     4561 ttaaggacgc gacggcgccc tcatagacga tcgtcacttt ggtgatcggc gaatcggtca
     4621 attggccggc gaacgagccg agccgctcgg cgagcgcgat gaacggcttc agcttcggcg
     4681 cctcctcggc ggtgatcgac gggaagttca ccgcattggc gatcgcgccg cgcatcaaat
     4741 agtccgacat ctgctcggcg acctggagcg cgacgttttc ctgcgcctcg gtcgtcgagg
     4801 cgccgagatg cggcgtcgcc accacgttgg gatggccgaa gagaggattg ctggtcgcag
     4861 gctcctcgac gaacgtgtcg aaagccgcgc cggcgacatg gccggaatcg agcgcggcgc
     4921 gcaaagcctt ttcatcgacc aggccgccgc gcgcgcaatt gacgatgcgg acgcctttct
     4981 tggtcttggc gatgttctcg gctgagagga tgttcttggt ctgcgccgtc atcggcgtgt
     5041 gcagcgtgat gaaatcggcg cgcttcaaca gatcgtcgag ttcgaccttc tcgacgccga
     5101 gctcggccgc gcgctcctcg ttgaggaacg gatcataggc gatcacccgc attttgagtc
     5161 cgatggcgcg ggtggcgacg atcgagccga tattgccgca gccgataatg ccgagaacct
     5221 tgccggtgat ctccaccccc atgaatctgt tcttctccca cttgccggcc tgggtcgagg
     5281 cgtcggcgct ggggatttgc cgcgccagcg cgaacatcag cgcgatggcg tgctcggcgg
     5341 tggtgatcga attgccgaac ggcgtgttca tcacgatcac gccgcgcgcc gtggccgcgg
     5401 ggatgtcgac attgtcgacg ccgatgccgg cgcggccgac gactttcaat ttttcggcgc
     5461 gttcgagcag cttggccgtg accttggtcg cagagcggat ggcgaggccg tcatactcgc
     5521 cgatgatctt ggcgagctcc tccttgtcga ggccgggctt cacgtcgaca tcgacgccgc
     5581 gctccttgaa gatcgcgacg gcggcggggg agagtttgtc tgagatgagt acgcggggca
     5641 tgggggagtc ctccgatcag gggatggccg tcattgcgag cgaagcgaag caatccaatc
     5701 gtaggcgcag ccccgatgag tggattgctt cgtcgcttcg ctcctcgcaa cgatggcgga
     5761 gaatcgggcg ttacgccgcc ttggcgagct tggctttctc ttcggcgaag gcgaagtcga
     5821 gccaatgggt cagcgcttcg acatcgctct tttcgatcgt cgcgccgcac cagatgcgta
     5881 ggccgggcgg cgcgtcgcgg tacgcgccga tatcgtaggc gaccttctcc ctttcgagga
     5941 gcgaggcgat cgccttggcg aaagccgcct gcgcctcggc cggcaaaccg gcgatggcgg
     6001 gatcgatcac cttcaggcaa acgctggtgt tcgagcgtgt cgccgggtcg gcggcgagga
     6061 aatcgatcca tggcgtcttc gccgcccaat cggcgatggt tttcagattg gcgtcggcgc
     6121 gcgcgatcag cgcgttcaag ccgccgattc gcttcgccca ttgcagcgcg tcgagatagt
     6181 cctcgacgca gagcatggac ggcgtgttga tcgtctcgcc ttcgaaaatc ccttcgatca
     6241 gcttgccgcc cttggtcatg cggaagactt tcggcagcgg ccaggccggc ttgtacgtct
     6301 ccaaccgctc gacggcgcgc gggctgagaa tgagcatgcc atgcgccgct tcgccgccca
     6361 gcgccttctg ccaggagaag gtgacgacgt cgagcttggt ccaatcgatc ttctgcgcga
     6421 aagcggccga agtggcgtcg cagatcgtca ggcccttgcg gtccgccttg atccagtcgg
     6481 cgttcggcac gcgcacgccg gatgtcgtgc cgttccaggt gaagacgacg tcgcgatcga
     6541 aattgacttg gctgaggtca ggcagttcgc cataggacgc cttcagcacg cgcgcatcga
     6601 gcttgagttg cttgacgaca tcggtcaccc agccttcgcc gaagctctcc caagcgagaa
     6661 tgtcgacgcc gcgcgcgccg agcagcgacc acagcgccat ttccactgcg ccagtatcgg
     6721 agccgggcgt aatgccgatg cgataatcgg ccgggacttg cagcacgtcg cgggtcaggt
     6781 cgatcgcgag tttcagcttg gccttgccga gcttggcgcg atgcgagcgg ccgagcgccg
     6841 cgtctttgag gatttcgggg gtccagccgg gccgtttagc gcaggggccg gaagaaaagc
     6901 gcgcgttatt cggccgcgcg gccggagcat tcgccatcgt tgatccatcc tttcagatgg
     6961 gcgcctctcg ttggggagag gtgtcccgct gacggcgata cgcgaacgag gcggctttcg
     7021 tcaagcagtt ttattgtcgc ggtcgaggct tgatccttca ggagtagttt ctttttaaca
     7081 cgcgccggcg cggggcgagg ctgtcgcgaa agtaccaagt tctctcaccc tgtcttgcgc
     7141 agcgacgcgc gaagcgtctc gtcgatccgc gattgccatc ccggcccctt cgccttgaag
     7201 gcttcgacga catcgcggct caaacggatt ga
//
LOCUS       DATGJW010000186         6820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1383180, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000186 DATGJW010000000
VERSION     DATGJW010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6820
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1383180"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            200..613
                     /locus_tag="VKU03_05620"
     CDS             200..613
                     /locus_tag="VKU03_05620"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLJ70775.1"
                     /translation="MTAAENKALIEDIMAARSRGDHAPFRAAMAEDFVWRIIGSTAWS
                     GEYVGKEDVRERLLKPLHAQFAAPTRITPTRILADGDFVVLQCEGDATTRAGERYANT
                     YCLVIRMQDGKMRELTEYMDTALVERVLAPPPWRG"
     gene            complement(759..1004)
                     /locus_tag="VKU03_05625"
     CDS             complement(759..1004)
                     /locus_tag="VKU03_05625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70776.1"
                     /translation="MPPSLRIGEIASRTGRSIHTIRWYGRALLADHRARVRETIERWG
                     EALALLDAKVDYYDEWVASGARPAVAPHRRVRRRGKI"
     gene            complement(1477..2235)
                     /locus_tag="VKU03_05630"
     CDS             complement(1477..2235)
                     /locus_tag="VKU03_05630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007564740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0043461 - proton-transporting ATP synthase
                     complex assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP12 family protein"
                     /protein_id="HLJ70777.1"
                     /translation="MSEEPGPMRAAQANMRPPLPKRFYISAGVAPREGGFALTLDGRG
                     ARTPAKNLLILPTRPLAEIIVAEWDAQGETIDPATMPATRLANSAIDGVQPKIPEVRL
                     EIAAYAGADLLCYRAGEPESLVEAQAAAWDPVLDWARDELGARFILSEGVRHVAQPEP
                     ALQAVGAALETIGDPFALAALHVMTTLTGSALLALAVARRRLSAEEAWRAAHVDEDFQ
                     ISRWGEDAEAAARRAARWREMAAAAGVYAAAGRG"
     gene            complement(2436..3437)
                     /locus_tag="VKU03_05635"
     CDS             complement(2436..3437)
                     /locus_tag="VKU03_05635"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918443.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLJ70778.1"
                     /translation="MRLDRWFKRRFPNLPLSHLNKIVRKGEVRVDGKRVETSTRLEAG
                     QSVRTPPLDIAPPAAARPPREDDAAAIRAMILYEDRGVIVLNKPYGLAVQGGSGTYRH
                     IDGMLAALADKNGQRPALVHRLDRDTAGVLLVAKTRRIAADLGEIFRSRGAKKIYWAL
                     VEGVPKPARGRISLFLAKGEAMGEDRAPRKAKRADFERMRVARHGEEDAQHSLTLYAV
                     VDKVPPRLAWLSMRPITGRTHQLRAHCEAIGHPIVGDPKYNRRPDNDPARRDPLRALP
                     PGLEPKLHLLARRLILPHPLGGTIDVAAPLPPHMQTSFDMFGFDVRTPDPIEDAPDE"
     gene            complement(3488..3871)
                     /gene="crcB"
                     /locus_tag="VKU03_05640"
     CDS             complement(3488..3871)
                     /gene="crcB"
                     /locus_tag="VKU03_05640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fluoride efflux transporter CrcB"
                     /protein_id="HLJ70779.1"
                     /translation="MQSIVLVFLGAGAGGVVRHVVNLLAARWWGTDFPLGTLLINVTG
                     SMLMGLLAGWLAFKASANWSQPLRLFVATGVLGGYTTFSTFSLETVLLIERNQIAAAA
                     GYVVFSLLFGFLGLWAALMLMRSLG"
     gene            complement(3963..4451)
                     /locus_tag="VKU03_05645"
     CDS             complement(3963..4451)
                     /locus_tag="VKU03_05645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HLJ70780.1"
                     /translation="MRHFRSFALAAALAFAAAPAFAQQFKVGDLVIEQPWTRATPKGA
                     SVAAGYLTIRNNGTSPDKLLGGSADFAETVQIHEMSNANGVMKMRRIDGLEIPAKGGV
                     TLKPNGVHLMFQGLKRPLVAGQTVKATLTFEHAGSAPVEFKIGGMGAMKPAGDSMGGM
                     KM"
     gene            complement(4478..4867)
                     /gene="copC"
                     /locus_tag="VKU03_05650"
     CDS             complement(4478..4867)
                     /gene="copC"
                     /locus_tag="VKU03_05650"
                     /inference="COORDINATES: protein motif:HMM:NF033814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis periplasmic binding protein
                     CopC"
                     /protein_id="HLJ70781.1"
                     /translation="MISSPRWARLSVAGMALVFLAPSQVSSAAAHALLKKAAPPPGGV
                     VSASPSEIRITFSEGVEPRFSTIALTTQTGAAAPLGKPSVDPNDDKTLVAPVAEPLKP
                     GVYIVHWHVVAADTHKTQGSFQFTIRP"
     gene            5535..6446
                     /locus_tag="VKU03_05655"
     CDS             5535..6446
                     /locus_tag="VKU03_05655"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HLJ70782.1"
                     /translation="MQASRFFLDRDDGAKIQIYRWAPNGAPRAAIQIAHGLCEHAGRY
                     ERLAAALTAAGYLTYASDHRGHGPSAAAGERGFFAEANGWRKCLDDLWAVNRRLAADA
                     PGLKIVFFGHSMGSIMGQQFIAEHGEALAGAALSGSNGPPPAILPLGRLLARFERLRL
                     GPRGHSPLLRHIIFGEYNKAFRPARTDFDWLSRDQAEVDRYCADPLCGFPFTVQLAID
                     MLDAVGPIASQATIARLPKSLPIYIFSGARDPVGANLQGLIDAYRRAGLAVTAKIYPG
                     ARHETLNETNRDEVVADLLAWIGRALG"
     gene            6543..>6820
                     /locus_tag="VKU03_05660"
     CDS             6543..>6820
                     /locus_tag="VKU03_05660"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase family
                     protein"
                     /protein_id="HLJ70783.1"
                     /translation="MIVREWRGRAKLDRADAYPKHFRESVAPGLKTIPGFLGAELCRR
                     LVGDRIEFLVVSRWRSMEAIRAFAGLDAAKAVVEPDAVAALEEYDDFVR"
BASE COUNT         1138 a         2322 c         2251 g         1109 t
ORIGIN      
        1 tggagccgct ccgcggtcaa gacccttgac aggggatttt ttcggcgctt gcgggccagc
       61 gttccaaaca atgacggatt gcgctcctcg gttaggcccc attttctacc tctttcagac
      121 cagcgacgcc taggcgcttg acctagagcg tgctctaggc cgtagagacg gcgactgttc
      181 caaaccagaa ggagcagtca tgacagccgc agagaacaaa gctctcattg aagacatcat
      241 ggcggcgcgg tcgcgcggcg atcacgcgcc gttccgggcc gctatggcgg aggatttcgt
      301 ctggcggatc atcggctcga cggcgtggtc gggcgagtat gtcggcaagg aggatgtccg
      361 cgagcgcctg ttgaagccgc tccacgcgca gttcgccgcg ccgacccgga tcacgccgac
      421 ccgcatcctg gccgatggcg atttcgtcgt cctccaatgc gaaggcgacg cgacgacccg
      481 ggccggcgaa cgctacgcca atacctattg cctcgtcatc cgcatgcagg acggcaagat
      541 gcgcgagctg accgaatata tggacacggc gctggtcgag cgggtcctcg ccccgccgcc
      601 atggcgaggt tgacgcgtac gagcggccgg aggaaagccc gcgtcattcc cgcgaaaccg
      661 ggaatccgga ctgagccggc cttctcgcgc cgcggttctc tggatgccca ctttcgcggg
      721 acacggcggc caaatcgcgg ccttcccgcg ggccccgcct atatcttccc ccgccgcctc
      781 acccgccgat gcggcgcgac agccggccgg gcgccgctcg cgacccactc gtcgtaataa
      841 tcgaccttcg cgtcgagcag agccagcgct tcgccccacc gttcgatcgt ctcgcgtacg
      901 cgcgcacggt ggtcggcgag cagcgcgcgg ccgtaccagc ggatcgtatg gatgctgcgg
      961 ccggttcgac tggcgatttc gccgatacgg agtgatggcg gcatagtccg tccgtgcatg
     1021 ttaggcgatc gtgaggacga tcaggccgag ccccgcctcc gcccgcacga ccccgcgctt
     1081 gcgaattcta gcaggaaagg acgccgcagt caggaagctc ctactgagaa gctcctcctg
     1141 aggaagagcg caatccgtca ttgtttggaa cgccgagcca tgtgcgccga gaaaataccc
     1201 tgtcgagggt cttgaccgcg gagcggctcc atagcccgtt ccgggctatg gagcccttga
     1261 caggggattt tctcggcgct tgcgggccag cgttcccaac aatgacggat tgcgctcccc
     1321 tgggtcgcag tcgtcagacc gatacggcgc cgccctcccc gtcattgcga ggagcggagc
     1381 gacgaagcaa tccaggaacc gcagaggctc ctggattgct tcgctgcgct cgcaatgacg
     1441 gcgtacctgc tttagttcgc ctcgtagttt gcctcgtcaa cccctcccgg ccgctgcgta
     1501 cacccccgcc gccgccgcca tctcccgcca ccgcgccgcc cgccgcgccg cggcctccgc
     1561 gtcctcgccc cagcggctga tctggaaatc ctcgtcgaca tgggcggcgc gccaggcctc
     1621 ttcggcgctg agccgtcggc gcgccacggc gagcgcgagc agcgcggagc cggtcagcgt
     1681 cgtcatcaca tgcagagcgg cgagcgcgaa gggatcgccg atcgtctcca gcgcagcgcc
     1741 gaccgcctgc agcgccggct cgggttgggc gacatggcgg acgccttcgg agaggatgaa
     1801 ccgggcgccc aactcgtcgc gcgcccagtc gagcaccggg tcccacgccg cggcctgcgc
     1861 ttcgaccagg ctttccggct cgccggctcg atagcagagc aagtcggccc cggcataggc
     1921 ggcgatctcg agacgtactt ccgggatttt cggctggacg ccgtcgatcg ccgaattggc
     1981 gaggcgggtc gccggcatgg ttgcgggatc gatcgtctcg ccctgcgcgt cccattcggc
     2041 gacgatgatc tcggccagcg gccgcgtcgg caggatcagc aggttcttcg ccggcgtgcg
     2101 cgcgccgcgg ccgtcgagcg tcagcgcgaa gccgccctcc cgcggcgcca cgcctgcgct
     2161 tatatagaag cgcttcggca gcggcggccg catattggcc tgcgccgcgc gcatcgggcc
     2221 gggctcctca gacatggcgg agtacctccc cttctccccg ccaagcgggg agaaggtggc
     2281 cgcgtagcag ccggatgagg ggcagcgctg agtctgggag cggcgttcgt agggggcgta
     2341 cctttcgagt cctgcgcagc ccttcatccg gcgcttcgcg ccaccttctc cccgccttgc
     2401 ggggagaagg caatgcgccg ccacggctcg taccctcact catccggcgc gtcctcgatc
     2461 gggtcaggcg tcctgacgtc gaagccgaac atgtcgaagc tcgtctgcat atgcggcggc
     2521 agcggggcgg cgacgtcgat cgtcccgccg agcggatgcg gcaggatcag ccggcgcgcc
     2581 agcagatgca gcttcggctc gaggccaggc ggcaaggcgc gcaaagggtc cctgcgcgcc
     2641 ggatcgttgt cgggccttcg attgtatttc ggatcgccga cgatcgggtg gccgatcgcc
     2701 tcgcaatggg cgcgcagttg atgcgtgcgc ccggtgatcg gccgcatcga cagccacgcc
     2761 aggcgcggcg gaaccttgtc gaccaccgcg taaagcgtca gcgaatgctg cgcgtcctct
     2821 tcgccgtgcc gggcgacgcg catgcgctcg aaatcggcgc gcttcgcctt gcgcggcgca
     2881 cgatcctcgc ccatcgcctc gcccttggcg aggaacagcg agatgcgccc gcgcgccggc
     2941 ttcggcacgc cctcgaccaa agcccagtaa atcttcttcg cgccgcgcga gcggaaaatc
     3001 tcgccgagat cggcggcgat gcgccgcgtc ttggcgacca gcagcacgcc ggccgtgtcg
     3061 cggtcgagcc gatggaccaa agccgggcgc tggccgtttt tgtccgccag cgcggcgagc
     3121 atgccgtcaa tgtggcgata cgtgcccgag ccgccctgca ccgcaagacc atagggcttg
     3181 ttgagaacga tcacgccgcg gtcttcgtaa aggatcatcg cgcggatcgc cgcggcgtcg
     3241 tcctcgcgcg gcggccgcgc cgccgcgggc ggcgcaatgt cgagaggcgg cgtccgcacg
     3301 ctctggccgg cttcgagcct tgtcgacgtc tcgacccttt tgccgtccac cctgacctcg
     3361 cctttgcgca cgatcttgtt gagatgcgac aaaggcagat tgggaaagcg ccgcttgaac
     3421 cagcggtcga ggcgcatgcc ggcctcgtcc tcgctcacat cgagcacggc gactttccgc
     3481 gaagagttca accgaggctc cgcatcagca tcagcgccgc ccacaagccg agaaagccga
     3541 agagaagcga aaagacgaca tagccggccg ccgcggcgat ctggttgcgc tcgatcagca
     3601 gcaccgtctc gagcgaaaag gtcgagaagg tcgtatagcc gccgagcacg ccggtggcca
     3661 cgaaaaggcg cagcggctgc gaccaatttg cgctcgcctt gaacgccagc cagccggcga
     3721 gcaggcccat cagcatcgag ccggtgacgt tgatcagcaa cgttccaagg ggaaaatccg
     3781 ttccccacca gcgcgcggcg agaagattga cgacatggcg gacgacgccg ccggccccgg
     3841 cgccgagaaa gacgagaaca attgactgca tcggccggta taaagcgcat gcgccgcgcc
     3901 gagacaaggg cggcgcagcc ctgcggccgc ggtcggcgag cgcggccaca gggcgcgccg
     3961 cgctacatct tcatcccgcc catggaatcg cccgccggct tcatcgcgcc catgccgccg
     4021 attttgaact caaccggcgc cgagccggca tgttcgaagg tcagcgtcgc cttcacggtc
     4081 tggccggcga caagcggccg cttcaggccc tggaacatca gatggacgcc attgggcttg
     4141 agcgtgacgc cgcccttggc gggaatttcg agcccgtcga tccgccgcat cttcatcacg
     4201 ccattggcgt tgctcatctc atggatttgc acggtttctg cgaagtcggc cgagccgccg
     4261 agaagcttgt cggggctcgt cccattgttg cgaatggtga gatagcccgc cgcgacggaa
     4321 gccccttttg gcgtcgcccg ggtccagggc tgctcgatga cgagatcgcc aaccttgaat
     4381 tgctgcgcaa aagcgggcgc ggcggcaaag gcgagcgcgg cggcgagagc gaaagagcgg
     4441 aaatgtctca tgaatgtctc ctcaagtgcg aacgccgtta aggccgtatc gtgaattgaa
     4501 agctgccttg cgtcttgtgc gtgtctgccg cgacgacatg ccagtgcact atgtacacgc
     4561 ccggtttgag cggctcggca acaggcgcga ccagggtttt gtcgtcattg ggatcgacgc
     4621 tcggcttgcc gagcggcgcc gcggcgcccg tttgcgtcgt cagcgcaatg gtggaaaagc
     4681 gcggctccac gccttcgctg aacgtgatcc tgatttcgct cggcgaagcg ctgacgacgc
     4741 cgccgggcgg cggcgccgct ttcttgagaa gcgcatgcgc ggcggccgaa gagacttgcg
     4801 acggcgccaa aaaaacgagg gccatgccgg ctacgctcaa ccgcgcccat cgaggggacg
     4861 agatcatgat tggaattcct tgatggagta aaaagggcgc gcgaatccgc tcaccggaat
     4921 tcgcgtgaat atgcgctttc agctcaacgc aaagagggcg gcccccgcgg ccgcgcggcg
     4981 gaaaagaata ccggcggcgc ggcggtcccg tctcgccaag aaaagcgcag ggcgacgcca
     5041 cgctgaggtt caacgcgctg ggcgatgctc ggcggcgcca ggagcgccaa gccgcaggca
     5101 aggccgcaca gggcgcaatc gccgcaacaa ggaggggaat ggtccggcgc gccggcatga
     5161 ccggtttcgg cgcagagggg aacctgctgg ccgacggccg cgctcagcgc cgccgccgcg
     5221 cgctcgccgg gagaagcggc cgcggccggc gcggcgctca atccgatttg caggataaaa
     5281 gccaaagcgg cgagccaggc gacggcgggc cgacgcgggc gagcccgcag ccgccgcgcc
     5341 gtctttcgcc ttgtgttgtc gcgcctcgcc atccctttaa acgacgctcc gccgatccgc
     5401 ctcttgcgaa agcggccatt ttgcacccgc cgcgccggca aacaagatcg ccggcgtcgc
     5461 agcccggctt cggccacgcc cttgctcgaa agccgcgatc gccgcaagct ctgcgtggag
     5521 ctcggggaac ggccatgcaa gcgtctcgtt tctttctcga tcgcgacgac ggcgcgaaaa
     5581 tccaaatcta tcgttgggcg ccgaacggcg cgccgcgcgc cgccattcag attgcccacg
     5641 ggctttgcga acatgccggt cgttacgagc gcctggcggc ggcgctgact gcggcgggct
     5701 atctgactta tgcgagcgat caccgcggcc acgggccgag cgcggcggcg ggcgaacgcg
     5761 gcttcttcgc cgaggccaat ggctggcgca aatgtctcga cgatctctgg gcggtcaatc
     5821 gccgcctcgc cgccgacgcg ccgggcttga agatcgtctt tttcggccat tcgatgggct
     5881 cgatcatggg ccagcaattc atcgccgagc acggcgaggc gctcgccggc gcggctctgt
     5941 ccggctccaa cggcccgccg ccggcgatcc tgccgctcgg ccgcttgctc gcccgcttcg
     6001 agcgtttacg gctcgggccg cgcggccata gcccgctgct ccggcacatc attttcggcg
     6061 aatacaacaa ggcgttccgt ccggcccgaa ccgatttcga ctggctgtcg cgcgatcagg
     6121 ccgaggtcga cagatattgc gccgatccgc tctgcggctt tccgttcacg gtccagctcg
     6181 ccatcgacat gctcgacgcg gtcggcccga tcgccagcca ggcgacgatc gcgcgcctgc
     6241 cgaaatcgct gccgatctat attttcagcg gcgcgcgcga tccggtcggc gccaacttgc
     6301 aaggcctgat cgacgcctat cgccgcgccg gcctcgccgt cacggcgaaa atctatcccg
     6361 gcgcgcgtca tgagacgttg aacgagacca atcgcgacga agtcgtcgcg gatttgctcg
     6421 cctggatcgg ccgcgccttg ggttagaaaa tatcgtcatt gcgagcggag cgaggcaatc
     6481 cagtcgcagc gcgccgaccg ctgaaatgtt cgcctcgcag ggacggagaa ggaggatttg
     6541 cgatgatcgt tcgagaatgg cggggccgcg cgaaacttga tcgcgccgac gcctatccga
     6601 agcactttcg cgagagcgtt gcgcctgggc tcaagaccat tccgggcttt ctcggcgccg
     6661 agctttgccg acgtcttgtc ggcgaccgga tcgagttcct tgtcgtctca cgctggcggt
     6721 cgatggaggc gatccgcgcc ttcgccggcc tcgacgccgc caaggccgtg gtcgagccgg
     6781 acgcggttgc ggcgctcgaa gaatacgacg atttcgtacg
//
LOCUS       DATGJW010000187         9912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_346869, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000187 DATGJW010000000
VERSION     DATGJW010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9912
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_346869"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..974
                     /locus_tag="VKU03_05665"
     CDS             <1..974
                     /locus_tag="VKU03_05665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ornithine cyclodeaminase family protein"
                     /protein_id="HLJ70784.1"
                     /translation="LLRTPLSGARKGDVYFDESHVRSKLAWDDLIAAMESSLAAFSRG
                     ETLQPLRTVLTVNEGKRFFGIMPAVAGDVMGLKAVTFYPNNQELGIPTHNGTILLFRT
                     DTGEPLAAMDGARLTQWRTAAASALAARYLAKSNARRLLMVGAGAMAPFLVRAHCAVR
                     PIAEVALWNHRRAGAERLAQILAGGGLRITIADDLEQAAREADVISCATLSPSPLIKG
                     DWLKGGQHVDLVGAFNLQMREADDEALRRARVFIDTEAALHEGGDVAVAIAKGAIAKS
                     HIVADLAALCAGATGRRSEDEITLFKSVGAAIEDLAAAMLVWRRRLS"
     gene            complement(1063..1947)
                     /gene="mmsB"
                     /locus_tag="VKU03_05670"
     CDS             complement(1063..1947)
                     /gene="mmsB"
                     /locus_tag="VKU03_05670"
                     /EC_number="1.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019197588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008442 - 3-hydroxyisobutyrate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006574 - valine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyisobutyrate dehydrogenase"
                     /protein_id="HLJ70785.1"
                     /translation="MTAIAFIGLGNMGGPMAANLVKAGHSVLGFDLVESSRNEAAAHG
                     VAIAGSGREAVAAAEIVVTMLPAGKHVLSVWDDILPAAGSGALFIDSSTIDVDSARRA
                     HRQAKDAGVLSLDAPVSGGVGGAKAATLTFMCGGADEAFARAKPILEKMGKRIVHCGE
                     AGAGQAAKICNNMILGATMIVTCEAFALAEKLGLSAQALYDVASTSSGQSWSLTSYCP
                     VPGPVPASPANNAYRPGFAAALMLKDLKLAQEAALAAGAATPLGAAAAQLYGLFAAAG
                     HAGDDFSGIINFIRGEGR"
     gene            complement(2027..3292)
                     /locus_tag="VKU03_05675"
     CDS             complement(2027..3292)
                     /locus_tag="VKU03_05675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011504605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ70786.1"
                     /translation="MTQTIADSQPSAPASAAAAPRRFNPRALDALNFLLADVRGAVGP
                     YLNVFLVTQQGWSQEAVGLVTTISGLIGLLFNAPAGALIDASRAKRGLVVVALALLGL
                     SGAAVGLFPSFWPLLAATTVMAVVGDLFGPAVAALTLGLFPRRQLARRMGRNSAFDHA
                     GNVAIALVAALVGWAFTQRAVFFLTPLFAALAAAAVLVIPAAAIDHERAREARRDGAA
                     QAASWTELLRHRPLVIFSLCTVLFHFANAPLLPLVGQKLALAHPAYATAMMSSCIVAA
                     QAIMLPIAVLVGRNADRFGRKPIFLIGFGVLPLRAFLYPVSDHSAWLIGVQLLDGVGA
                     GIFGALMPLVLADIMRGTGRYNLAQGAVAAALGAGASLSGLCAGILVDHFGYSTTFVT
                     LGAVALVALAVFALLMPETKRSGAESGQA"
     gene            complement(3361..4611)
                     /locus_tag="VKU03_05680"
     CDS             complement(3361..4611)
                     /locus_tag="VKU03_05680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenic transporter"
                     /protein_id="HLJ70787.1"
                     /translation="MTSETATLSIAAIATAAVIARPWNLPEAIWAIAGAVLLVAFGLL
                     PLNDALAGIAKGTDVYLFLIGMMLLAELARQEGLFDWLATHAVNYARGSPRRLFALIY
                     GVGVIVTVFLSNDATAVVLTPAVYAAARAAGAEPLPYLLICAFIANAASFVLPISNPA
                     NLVVFGAGMPPLASWLARFALPSIASIALTFLALRFALRRQLRKPLKGAPETAPLTRG
                     GALAAIGVAATALALIAASALGWRLGLPTFIAGVLTAALILIGARQSPWPIVKGVSWS
                     VLPLVAGLFVLVEALDHTGVIATLARLTGDYAGRSVSLTGAAAGALVALACNLMNNLP
                     VGLIVGSTVALDHLPHPIVAAALVGVDLGPNLSITGSLATILWLAALRREGQGVNAWR
                     FLKLGVLVMPPALIAALAAAILIG"
     gene            complement(4734..5369)
                     /locus_tag="VKU03_05685"
     CDS             complement(4734..5369)
                     /locus_tag="VKU03_05685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685523.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70788.1"
                     /translation="MARSIMHWINPDCLPEIEGRFERFLLNPHGEADGMILAGGVEVH
                     FPPHLAGALRAALEEDAAGPLRIRGVRPRRGDVFAAVAFETAKGARIIDNGPPKKDER
                     EKGDKRGREKRAPMAIDGVVRRPLHGPKGEARGALLEDGRIVRFAPDEAPRLKALLTP
                     GARLSARGEGLATALGTVVEAREIGPSAAALQPVKAKAPKPDKPRGHEAYA"
     gene            complement(5516..6835)
                     /locus_tag="VKU03_05690"
     CDS             complement(5516..6835)
                     /locus_tag="VKU03_05690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLJ70789.1"
                     /translation="MRQVLIVDDDEAMRDALAEAARDLGFAPRLAPSGAEALVMLAEA
                     PADAVLLDLRMPGLDGLEVLKRIRALPRPPPVTVLTAHATAENTIEAMRLGAFDHLTK
                     PVGRDALKRALDAMAAAPAGPEAAAAEPGGVGLIGGTEAIRTVQKAIGMLADSDATVL
                     ITGETGTGKELVARAIHDHGQRRGKPFVAVNCAAIPNELLESELFGHVRGAFTGAASD
                     RLGAFREAAAGTLFLDEIGDMDVAMQAKILRALQERVVTPVGGKPAPVDVRVIAATHR
                     DLSQRVREGSFREDLFYRLHVVPIHLPALRERIADIVPLAEHFLKSAGKTLSADAAAR
                     LIGHAWPGNVRELKNAMERAAILARGDVVAVADLNFIGEKPPAEPQALAWPDEDLASA
                     IERLEKMLIRRALIRHGGNRAAASRALGVHRQLLYAKIKRYGLEDVR"
     gene            complement(6820..8280)
                     /locus_tag="VKU03_05695"
     CDS             complement(6820..8280)
                     /locus_tag="VKU03_05695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLJ70790.1"
                     /translation="MARSLRGRFIVLLALLIAAAASAGALMFGLFEQSASAQAGQAEA
                     EIGRACDAIESAYRSFAAAWPAQPDEPALSRDLTMVVDAALRERAGVEGGVFRAGAQS
                     LAYAFPTYQGGGPKTDLPAAEAPRIEAANRAALAENRPLVERFDASTQILLIAACPLP
                     GPIPQLTGWAMTRVFTFAGSSYWLLMAGLGVLLASVLAAAALLTRLTLLWSRHVGRIE
                     AALKTYDVAELPALPITGERELDRIVAALNEAGRRLALARQTADRLARQLAAGERLAA
                     IGRLAAGLAHEIRNPIAAMRLKAENAAAGDAARKDQALAAIVGQIDRLDALLRRLLSV
                     TARDKPKPVVVKLAPFLQACVAAHDEFARAKHLSLQWRSEVETAEFDPDRVRSALDNL
                     VLNAIQAAPKNSAVLLRAARAGEALTLSVHDEGDGPAATIRENLFEPFVTGRADGVGL
                     GLSIVREVAAAHGGEARLAETPSGATFEIVLPCAKS"
     gene            complement(8328..8756)
                     /locus_tag="VKU03_05700"
     CDS             complement(8328..8756)
                     /locus_tag="VKU03_05700"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ70791.1"
                     /translation="MSDAVTDHRPPPETILFVEDEVLTRMDMAEFLRQSGYRVSEAAS
                     AAEAIDALGSKLAVDLVITDVRMSGDMDGFALAEWVRTNRPGVEVIVTSGDPHSKERA
                     EQSPVGAFLAKPYTGRALLDLVTQALAKRAPDLGGAAGAA"
     gene            complement(8818..9483)
                     /locus_tag="VKU03_05705"
     CDS             complement(8818..9483)
                     /locus_tag="VKU03_05705"
                     /inference="COORDINATES: protein motif:HMM:NF025605.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ70792.1"
                     /translation="MSEGETQLERGGFRRGPGGRPTRQEAERRHRELLETTKRLLLER
                     GWDGTSIDEISRQSGVAKRFLYARYPDKASLFVAAVAQFRDEQIGALGALEAPPADVE
                     QGLLELGRRLMAVALREETLAIVRLFLAEAPRMRDYAKMFAESFPRRGLSGISRFMAV
                     YAERGVLALDDPELAAQHFAMLVIGAPQRLAMLGHRDSPEKEERRLRSAVTLFLDGCR
                     RRP"
     gene            9552..>9912
                     /locus_tag="VKU03_05710"
     CDS             9552..>9912
                     /locus_tag="VKU03_05710"
                     /inference="COORDINATES: protein motif:HMM:NF024923.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-binding protein"
                     /protein_id="HLJ70793.1"
                     /translation="MRRGPLLAVAVIAAALAAWFFLLRPQKPRPVEWQGYADADFVKV
                     GPTQAGLLVALHVRRGDKVTKGEPLFDQDDANDRAAVDQAQRQLAQARDQLANLKSGG
                     KPTEIAEAEANIADAEAA"
BASE COUNT         1623 a         3335 c         3532 g         1422 t
ORIGIN      
        1 tgctacttcg tactccacta agcggcgcgc ggaaaggcga cgtgtacttc gacgaatcgc
       61 acgttcgcag taagctcgca tgggacgatc tcatcgcggc gatggaatcg tccctcgccg
      121 ctttttcccg cggcgaaacc ctgcagccgc tgcgcaccgt gcttaccgtt aacgaaggga
      181 aacgcttttt cgggatcatg cccgccgtcg ccggcgacgt gatggggctc aaagcggtga
      241 cgttctatcc caacaatcaa gagctgggca tccccacgca caacggcacg atactgctct
      301 tccgcaccga taccggcgag ccgctcgccg cgatggacgg cgcgcggctg acgcaatggc
      361 gcaccgccgc cgcctcggcg ctggcggcgc gctatctcgc caaaagcaac gcgcgccgcc
      421 tgctgatggt cggcgccggc gcgatggccc ctttcctcgt cagggcgcat tgcgcggtgc
      481 ggccgatcgc cgaagtcgcg ctttggaacc atcgccgcgc cggcgctgag cgcttggcgc
      541 aaatactggc cgggggcggc ttgcgtataa caatcgccga cgacctggag caggcggcgc
      601 gcgaagcgga cgtgatttcc tgcgcgacat tgtcgccctc gcccctcatc aaaggcgatt
      661 ggctcaaagg cggccagcat gtcgacttgg tcggcgcgtt caatctccag atgcgcgaag
      721 ccgacgacga ggctttgcgc cgcgcgcgcg tcttcatcga cacggaggcc gctttgcatg
      781 aaggcggcga cgtcgccgta gcgatcgcga agggcgcaat cgccaagagc catatcgtcg
      841 ccgatctcgc ggccctctgc gccggcgcaa cgggacggcg aagcgaggac gagatcacgc
      901 tgttcaagtc cgtcggcgcc gcgatcgagg atttggcggc ggcgatgctg gtctggagac
      961 ggcgcctctc gtaaaaccgt cattggcagc aaagcaatcc gttcataggg cgccgctcga
     1021 cgaatggatt tgcccgtcgc tgcgctcctc gcaacgacgg gattaccgtc cttcgccgcg
     1081 gatgaaattg atgatccccg aaaagtcgtc gccggcatgg ccggcggcgg cgaacaggcc
     1141 gtagagctgc gccgcggcgg cgccgagcgg cgtcgcggcg ccggcggcga gcgcggcttc
     1201 ctgcgcgagc ttcaaatcct tcagcatcag ggcggcggcg aagccgggcc gataggcgtt
     1261 gttggcgggc gacgccggaa ccgggccggg aaccgggcaa tagctggtca gcgaccagga
     1321 ttggcccgac gaggtcgagg cgacgtcgta gagcgcctgc gcggaaaggc cgagcttctc
     1381 ggcaagcgcg aacgcttcgc aggtgacgat catcgtcgcg ccgagaatca tgttgttgca
     1441 gattttcgcc gcctggccgg cgccggcctc gccgcaatgg acgatgcgct tgcccatctt
     1501 ctcaaggatc ggcttggccc tggcgaaggc ctcgtcggcg ccgccgcaca tgaaggtgag
     1561 cgtcgccgcc ttggctccgc cgacaccgcc cgagaccggc gcgtcgagcg aaagcacgcc
     1621 ggcatccttc gcctgccgat gggcgcgccg ggcgctgtcg acgtcgatgg tcgaggaatc
     1681 gatgaacagc gcgccgcttc cggcggccgg cagaatatcg tcccagaccg aaagcacatg
     1741 ctttcccgcc ggcagcatgg tgacgacgat ctcggcggcg gcgaccgctt cgcggccgga
     1801 gccggcgatg gcgacgccat gcgccgccgc ctcgttgcgg gaggattcga cgagatcgaa
     1861 gccgaggacg ctgtggccgg ccttgaccag attggcggcc atcgggccgc ccatattgcc
     1921 gaggccgatg aaagcgattg cggtcatgtg cgtctcctcc gccccgtcag gctttgcaaa
     1981 cgagtttgca accgccgcgg cgacgccgtc cagaggcgat cgcgccttaa gcctggccgg
     2041 attcggcgcc gctgcgcttt gtctccggca tgagcagcgc gaagacggcg agggcgacga
     2101 gcgccaccgc gccgagcgtg acgaaggtcg tcgaatagcc gaaatgatcg acgagaattc
     2161 ccgcgcaaag cccgcttaag gacgcgccgg cgccgagcgc cgcggcgacc gcgccctgcg
     2221 ccagattgta gcggccggtg ccgcgcatga tgtcggcgag aaccagcggc atgagcgcgc
     2281 cgaaaatccc cgcgccgacg ccgtcgagca attgcacgcc gatcagccag gcgctgtggt
     2341 ccgacactgg atagaggaaa gcgcgcagcg gcagcacgcc gaagccgatc aggaagatcg
     2401 gcttgcggcc gaagcggtcg gcgttgcggc cgacgagaac cgcgatcggc agcatgatcg
     2461 cctgcgccgc gacgatgcat gacgacatca tcgcggtcgc gtaggccgga tgcgccagcg
     2521 ccagcttctg cccgaccagc ggcagaagcg gcgcattggc gaaatgaaaa agcacggtgc
     2581 agagcgaaaa gatgacgagc gggcggtgac gcaaaagctc ggtccaggac gccgcctgcg
     2641 ccgcgccgtc gcggcgcgcc tcacgggcgc gctcgtggtc gatcgccgcc gccggaatga
     2701 cgagaaccgc cgcggcggcg agcgcggcga agagcggcgt caggaagaac acggcgcgct
     2761 gcgtgaacgc ccagccgacc aaagcggcga cgagcgcgat ggcgacattg ccggcatgat
     2821 cgaaggccga gttgcgtccc atccgccgcg ccaattggcg gcgcgggaag agcccgagcg
     2881 tcagcgccgc caccgccggg ccgaacaggt cgccgacaac cgccatgacg gtcgtggcgg
     2941 cgagcagcgg ccagaagctc gggaagagcc cgaccgcggc gccgctcagg ccgagcaagg
     3001 cgagcgcgac gacgacgagg ccgcgcttgg cgcggctggc gtcgatcaac gcgccggccg
     3061 gcgcgttgaa gagaaggccg atcaggccgc tgatcgtggt gacgaggccg accgcctcct
     3121 ggctccagcc ctgctgggtg accaggaaca cgttgagata gggaccgacc gcgccgcgga
     3181 cgtcggcgag cagaaagttc agcgcgtcga gggcgcgcgg attgaagcgg cgcggcgcgg
     3241 ccgctgcgct cgccggcgcg cttggctggc tgtcggcgat ggtttgcgtc atgacgcgcc
     3301 tcgctgatgt cgaaacggcg cgtcctggcg atctgtgcgt cgcggcccag cgcttgacgg
     3361 tcagccgatc aggatcgccg cggcgagcgc ggcgatcaaa gcgggcggca tgaccaggac
     3421 gccgagtttc aggaatcgcc aggcgttgac cccctggcct tcgcggcgca gcgcggcgag
     3481 ccacaggatc gtcgccaaag agccggtgat cgagaggttg gggccgagat cgacgccgac
     3541 cagagccgcc gcgacgatcg ggtgcggcag atggtcgagc gccacggtcg agccgacgat
     3601 caggccgacc ggcaggttgt tcatgaggtt gcaagccaag gcgacgagcg ccccggccgc
     3661 ggcgccggtc aacgagaccg agcggccggc atagtcgccg gttagacgcg cgagcgtggc
     3721 gatgacgccg gtatggtcga gcgcctcgac cagcacgaac aggccggcga cgagcggcag
     3781 cacgctccac gacacgccct tgacgatcgg ccagggcgat tggcgcgccc cgatcaggat
     3841 gagcgccgcc gttaagacgc cggcgatgaa agtcggcaag cccaggcgcc agccgagcgc
     3901 cgaggcggcg atcagcgcca gcgcggtcgc ggcgacgccg atcgccgcca gcgcgccgcc
     3961 gcgggtcagc ggcgcggtct cgggcgcgcc cttgaggggc ttgcggagct ggcggcgcaa
     4021 ggcgaagcgc agcgcgagga aggtcagcgc gatggacgcg atcgacggca aggcgaagcg
     4081 cgccagccat gaagcaagcg gcggcatgcc ggcgccgaac acgacgaggt tcgccggatt
     4141 cgagatcggc aggacgaaac tcgcggcgtt ggcgatgaag gcgcagatca ggagataggg
     4201 cagcggctcg gccccggcgg cgcgcgccgc ggcgtagacg gcgggcgtca gcaccaccgc
     4261 cgtcgcgtca ttggagagaa aaacggtgac gatcacgccg acgccataga tcagggcgaa
     4321 gaggcggcgc ggcgagccgc gcgcatagtt gaccgcgtgg gtggcgagcc agtcgaacag
     4381 gccttcctgg cgcgcgagct ccgcgagcag catcatgccg atgaggaaga gatagacatc
     4441 cgtccctttg gcgatcccgg cgagcgcatc gttgagcggc agcaacccga aagcgacaag
     4501 cagcacggcg ccggcgatcg cccagatcgc ctcgggcagg ttccagggcc gcgcgatcac
     4561 ggccgcggtc gcgatggcgg cgatgctcaa agtcgccgtt tcagaggtca tcgccggctc
     4621 cgattccagg cccggccgcg gcaagcgaag gggcgcccct cgcgatgggc gtcgtcatcg
     4681 cgtccttcct gaacgagtcg cgctcgccgg cggtcgccgg cgagcgcgtc gaatcacgca
     4741 tacgcttcat ggccgcgcgg tttatcaggt ttgggcgctt tggccttgac cggctgaagc
     4801 gcggccgccg acgggccgat ttcccgcgcc tcgaccaccg tgccgagagc cgtcgccagc
     4861 ccctcgccgc gcgccgacag cctcgcgccg ggcgtcagca gcgctttcag gcgcggcgct
     4921 tcgtccggcg cgaaacggac gatgcgcccg tcttcgagca aagcgccgcg cgcctcgcct
     4981 ttcgggccat gcaagggccg gcgcacgacg ccgtcgatcg ccatgggcgc gcgcttctcc
     5041 ctcccgcgct tgtcgccctt ctctcgctcg tccttctttg gcggaccgtt gtcgatgatg
     5101 cgggcgccct tcgcggtctc gaacgcgacg gcggcaaaga cgtcgccccg ccgcggccgc
     5161 acgccccgga tgcgcagcgg accggccgcg tcctcctcca aggccgcgcg cagcgcgccg
     5221 gcgaggtgag gcggaaaatg cacctcgacg ccgccggcga ggatcatgcc gtccgcttcg
     5281 ccatgcggat tgagcaggaa gcgttcgaac cgcccttcga tttcaggcag gcaatcggga
     5341 ttgatccaat gcatgatcga cctcgccatt ggcgtgaatg cgccggcgcg attttgggtc
     5401 cccggcgcga ctgcaagatc gcaagcgccg cgccacgcct aaagcattga aaactttggc
     5461 tgagaaatat agctgacgcg gttagcgtcc gcttttccga cgcttcgccg tccgctcagc
     5521 ggacgtcctc gaggccgtag cgcttgatct tggcgtagag gagctggcga tggacgccga
     5581 gcgcgcgcga agcggcggcg cggttgccgc cgtgtctgat cagcgcgcgg cggatcagca
     5641 ttttttcgag gcgctcgatc gccgaggcga ggtcctcgtc gggccaggcc agcgcttgcg
     5701 gctcggcggg cggcttttcg ccgatgaaat tgagatcggc gacggcgaca acatcgccgc
     5761 gggccaggat cgccgcgcgc tccatggcgt ttttcagctc gcggacgttg cccggccagg
     5821 catggccgat caggcgcgcg gcggcgtcgg ccgaaagcgt cttgccggcg cttttgagga
     5881 aatgctcggc gagcgggacg atgtcggcga tgcgctcgcg caaggccggc agatggatcg
     5941 gcacgacgtg caggcgatag aagagatctt cgcggaagct tccttcccgc acgcgctggc
     6001 tgagatcgcg atgcgtggcg gcgatgacgc gcacgtcgac gggcgccggc ttgccgccga
     6061 ccggcgtcac cacccgttcc tgcaaggcgc ggaggatttt cgcctgcatg gcgacgtcca
     6121 tgtcgccgat ctcgtcgaga aacagcgttc ccgccgccgc ctcgcggaat gcgccgagcc
     6181 ggtcgctcgc cgcgccggtg aaggcgccgc ggacatggcc gaacaattcg ctctccaaca
     6241 gctcgttcgg aatcgccgcg caattgaccg cgacgaaggg cttgccgcgg cgctggccgt
     6301 gatcgtggat ggcgcgggcg acgagctcct tgccggttcc cgtctcgccg gtgatcagca
     6361 cggtcgcgtc gctgtcggcg agcatgccga tggccttttg caccgtgcgg atcgcctccg
     6421 tgccgccgat cagaccgacg ccgcccggct cggcggcggc tgcttcgggg ccggccggcg
     6481 cggcggccat cgcgtcgagc gcgcgcttca aggcgtcgcg gccgaccggc ttggtcaaat
     6541 ggtcgaacgc gccgagccgc atcgcttcga tcgtgttctc cgccgtggcg tgggcggtca
     6601 ggacggtgac cggcggcggc cgcggcaagg cgcggatgcg cttgaggact tcgagaccgt
     6661 cgaggcccgg catgcgcaaa tcgagcaaca ccgcgtcggc cggcgcctcg gcgagcatga
     6721 cgagcgcctc cgcgccggac ggcgcgaggc ggggcgcgaa gcccaaatcg cgcgccgcct
     6781 cggcgagcgc gtcgcgcatc gcctcgtcgt cgtcgacgat caggacttgg cgcatggcag
     6841 cacgatctcg aatgtcgcgc cggacggcgt ttcggcgagg cgcgcttccc cgccatgcgc
     6901 cgccgccact tcgcgcacga tcgacaggcc gagaccgacg ccgtcggcgc ggccggtgac
     6961 aaagggctcg aacagatttt cgcgaatcgt tgcggccggc ccgtcgccct cgtcgtgaac
     7021 cgagagggtg agcgcctcgc cggcgcgcgc cgcccggagg agcacggccg aatttttcgg
     7081 cgcggcctgg atggcgttga ggacgagatt gtcgagcgcg ctgcgcacgc ggtcgggatc
     7141 gaactcggcc gtttcgacct cgctgcgcca ttgcagcgac aggtgcttgg cgcgggcgaa
     7201 ttcgtcatgc gcggcgacgc acgcttgcaa gaacggcgcg agcttgacga cgaccggttt
     7261 cggcttgtcg cgcgccgtca cgctgagcag gcggcgcagc aaggcgtcga gccggtcgat
     7321 ctggccgaca atcgccgcga gcgcctggtc cttgcgggcg gcgtcgccgg cggccgcgtt
     7381 ctccgccttg agccgcatcg cggcgatcgg attgcggatc tcatgggcga ggccggcggc
     7441 aagccggcca atcgccgcca ggcgctcgcc ggccgccaat tggcgcgcca gccggtcggc
     7501 tgtttggcgc gcgagcgcca gacggcggcc cgcttcgttc agcgccgcga cgatgcggtc
     7561 caattcgcgc tcgccggtta tcgggagagc cggcagttcg gcgacgtcat aggtcttcag
     7621 cgccgcttcg atgcgcccga catggcgcga ccaaagcagg gtcagccggg tcagaagcgc
     7681 cgccgcggcg agcaccgagg cgagcagcac gcccaatccg gccatcagca gccagtagct
     7741 gctgccggcg aaggtgaaga cgcgcgtcat cgcccagccg gtcaattgcg ggatcgggcc
     7801 gggcagcggg caggcggcga tgaggaggat ttgcgtcgag gcgtcgaacc gctcgaccaa
     7861 cgggcggttt tcggccagcg cggcgcgatt ggctgcctca atgcgcggcg cctccgcggc
     7921 gggcaggtcg gtctttgggc cgccgccctg gtaggtcggg aaggcgtagg cgagcgattg
     7981 cgcgccggcg cggaagacgc cgccctcgac gccggcgcgc tcgcgcaagg cggcgtcgac
     8041 caccatggtc aagtcgcggc tcagcgccgg ctcgtccggc tgcgccggcc aggcggcggc
     8101 gaaggagcgg taggcgcttt cgatcgcatc gcaggcgcgg ccgatctcgg cttcggcctg
     8161 ccccgcctgc gcgctcgccg attgctcgaa aaggccgaac atcagcgcgc ccgccgaggc
     8221 ggcggccgcg atcaacagcg cgagaaggac aatgaaccgg ccccgcagag atcgagccat
     8281 aggtctcttc tgcgcatccg ttgattgtgc ggcgtcgaag agcggtttta agccgcgccg
     8341 gccgccccgc caaggtccgg ggctcgcttg gcgagcgcct gagtgacgag gtcgagcaag
     8401 gctcgccctg tatagggctt ggccaggaag gcgccaacgg gcgattgctc ggcccgctct
     8461 ttcgagtgag gatcgccgga cgtgacaatc acctcaacgc ctggacggtt cgtcctgacc
     8521 cattcagcca aggcgaagcc gtccatatcg ccggacatcc tcacgtcggt gatgacgaga
     8581 tcgacggcga gtttcgagcc gagcgcgtca atcgcttcag cggcgctggc ggcttcgctg
     8641 acccggtaac ccgactggcg gaggaactcc gccatgtcca tcctggtgag aacttcgtct
     8701 tcaacgaaaa ggatagtttc gggcggcggc cgatgatcgg ttactgcgtc gctcattcaa
     8761 cccctcgccc agctgggaaa actcaaacgc gccgcggcgg agatttgttc cgggcggtca
     8821 gggccgccgc cggcagccgt cgaggaacag cgtgaccgcc gaccgcaagc ggcgctcctc
     8881 cttttccgga ctatcgcgat ggccgagcat ggccaggcgc tgcggcgcgc cgatgaccag
     8941 catcgcgaaa tgctgggcgg cgagttcggg atcgtcgagc gccaggacgc cgcgctcggc
     9001 atagacggcc atgaagcggg agattcccga aaggccgcgg cgcggaaagc tctccgcgaa
     9061 cattttggcg tagtcgcgca tgcgcggcgc ctcggccagg aacaggcgga cgatcgccag
     9121 ggtctcctcg cgcagcgcca cggccatcag ccgccggccc agttcgagga gcccctgctc
     9181 gacatcggcc ggcggcgcct ccagggcgcc gagcgcgccg atctgctcgt cccggaactg
     9241 cgccacggcg gcgacgaaga gcgaggcctt gtcgggatag cgagcgtaaa gaaagcgctt
     9301 ggcgacgccg gactggcggg aaatctcgtc gatcgaggtt ccgtcccaac cgcgctccag
     9361 aagcaggcgc ttggtcgttt cgaggagctc gcggtggcgc cgttcggcct cctgacgggt
     9421 cggcctgccg cccggaccgc gtcggaagcc gcctctctca agctgggttt ccccctcgga
     9481 cattctcgcc ttgccggctc ctggggcgga tgataatgta actatatcgt ttcgttgcaa
     9541 taggtcaagc cgtgcgtcgt gggccgctcc tcgccgtcgc cgtgatcgcc gccgcgcttg
     9601 cggcgtggtt cttcctgctg cggccgcaaa agccccgccc cgtcgaatgg cagggctatg
     9661 ccgacgccga tttcgtcaaa gtggggccga cccaggccgg cctgctcgtc gccctccacg
     9721 tccggcgcgg cgacaaagtg acgaagggcg agccgctctt cgatcaagac gacgccaacg
     9781 atagggcggc ggtcgaccag gcgcagcggc aattggcgca ggcgcgcgac caactcgcca
     9841 atctcaaaag cggcggcaag ccgaccgaga tcgctgaggc cgaggccaat atcgccgacg
     9901 ccgaggcggc gc
//
LOCUS       DATGJW010000188         4110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1173350, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000188 DATGJW010000000
VERSION     DATGJW010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4110
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1173350"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            7..660
                     /locus_tag="VKU03_05715"
     CDS             7..660
                     /locus_tag="VKU03_05715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030428 - cell septum [Evidence IEA];
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family nucleotide pyrophosphatase"
                     /protein_id="HLJ70794.1"
                     /translation="MSEPAQRPKLVLASASVRRLALLAQMGLDPAAIIPADIDETPRR
                     GESPRALVQRLASEKTAVAVKVAQSRSELGRCLVLAADTVVCVGRRALPKCEVVEEAA
                     TCLRMLSGRAHRVYTAIALTTASGATRRRLVETRVRFKRLSRDEIDAYLASGEWRGKA
                     GGYAIQGLAGAFVVRLVGSYSCVVGLPLVETMGLLAGEGLPAYASWAAAGERALVNA"
     gene            661..888
                     /gene="yacG"
                     /locus_tag="VKU03_05720"
     CDS             661..888
                     /gene="yacG"
                     /locus_tag="VKU03_05720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase inhibitor YacG"
                     /protein_id="HLJ70795.1"
                     /translation="MTKSPANDNRSDKAPACPICGRPRRAEHRPFCSRRCADVDLHRW
                     LAAAYVIAGGEPAEDETAAPMRRPKGPDAKN"
     gene            965..1444
                     /locus_tag="VKU03_05725"
     CDS             965..1444
                     /locus_tag="VKU03_05725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF992 domain-containing protein"
                     /protein_id="HLJ70796.1"
                     /translation="MSKFFQISVAAGACALPVFLSAMPAQAQEGIKTGSLVCNVSGGI
                     GYLVGSRKAVHCTFTGTNGRYEHYIGHITKVGLDIGATTGGQIVWAVFEPTARHASLG
                     GNYAGVTAQATFGAGLGANVLIGGSNKAVALQPLSVEGQTGLNLAAGIGGLHLTRTR"
     gene            1559..2617
                     /locus_tag="VKU03_05730"
     CDS             1559..2617
                     /locus_tag="VKU03_05730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018242758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ70797.1"
                     /translation="MEYRQLGHSGLKVSALAMGTMTMGGRGNFSKVGSTGLDEARRQI
                     DLCIDAGVNLIDTANVYSGGASEEIIGEALGGKRKGGVLIATKARFPMGDGPNDRGLS
                     RHHLIRECEASLKRLRTDVIDLYQVHQWDGLTPLEETMEALDSLVRSGKVRYVGCSNF
                     SGWHVMKAMEIARRDGRVPFVSQQIHYTLQAREAEYELVPISIDQGLGILVWSPIAGG
                     LLSGKHRRNRQAPEGTRQFAGWKEPPIYDEGKLWDIVDAIVAIADSRGVSGAQVSLAW
                     LLGRPGVTSVIIGGRTEAQFKDNLAAVELKLTPDERKRLDAVSRPPLIYPYWHQSWTA
                     KDRLGAADLALHAAYLEK"
     gene            complement(2710..4035)
                     /locus_tag="VKU03_05735"
     CDS             complement(2710..4035)
                     /locus_tag="VKU03_05735"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HLJ70798.1"
                     /translation="MPARFDLILEGGIVVNQDGVGARDIGVVGERIAAIGPLGQAAAG
                     ERIDCRGLHILPGVIDSQVHFREPGATHKEDLETGSAAAVLGGVTAVFEMPNTDPLTV
                     SAAALEDKLGRARSRMHCDFAFWVGGTHDNVRDLTDLERLPGAAGIKVFMGASTGKLL
                     VPDDAGVLAILKNIRRRAAFHSEDEERLNERKPLRVKGDPASHPVWRDEIAALKATQR
                     LVALARQARARIHVLHITTAEEIAFLESIKDVATCEATPQHLTFSAEDYARLGTRLQM
                     NPPVRAAHHRDAIWRGVEQGIIDVLGSDHAPHTLEEKAKPYPESPSGMTGVQTLVPIM
                     LDHVANGRLTLQRFVDLTSAGPQRLFGIANKGRIAAGYDADFTIVDLKRRATISDSWI
                     ASRSRWTPYDGKNVTGWPVGTVIRGQRVMWEGEIVTPSRGKAVRFVEAL"
BASE COUNT          677 a         1366 c         1375 g          692 t
ORIGIN      
        1 aagatcatga gcgagccggc tcagcgccca aaacttgttc tggcgtcggc ttcggtgcgg
       61 cggctcgcgt tgctcgctca gatgggactc gatcccgccg cgatcattcc ggccgatatt
      121 gacgagacgc cacggcgcgg cgagtcgccg cgcgctctcg tccagaggct ggcgagcgaa
      181 aaaacggcgg tggcggtcaa agtcgcgcaa agccgcagcg agctcggccg ctgcctcgtg
      241 cttgccgccg acacggtcgt ctgtgtcggc cgccgcgcct tgccgaaatg cgaagtcgtc
      301 gaggaggccg cgacatgcct gcgcatgctg tccggccgcg cccaccgggt ctataccgcg
      361 atcgccttga cgacggcgag cggcgcgacg cgacgccggc tggtcgaaac gcgggtccgg
      421 ttcaagcgcc tgtcgcgcga tgagatcgac gcctatctgg cgagcggcga atggcgcgga
      481 aaggctggcg gctacgccat tcagggcctt gccggcgcgt ttgtcgtgcg tctggtcggc
      541 tcctatagtt gcgtggtcgg attgccgctg gtcgagacga tgggcctgct cgccggggag
      601 ggcctgcccg cgtacgcctc ctgggcggcg gccggcgaac gcgccttggt gaacgcctag
      661 atgaccaaat ctccggccaa cgacaaccgg tccgacaagg cgccggcctg cccgatttgc
      721 ggccggccgc gccgcgccga gcaccgcccg ttctgctcgc gccgctgcgc cgacgtcgat
      781 cttcatcgct ggctcgccgc cgcttatgtg attgcaggcg gcgaacctgc ggaagacgag
      841 accgccgctc cgatgcgccg gccaaaggga ccggacgcaa aaaattgacg ccgccgacga
      901 aaagtcgccg caaatcacgc gcccgataag ttgttgaagc aattccgctc tatcggaggc
      961 agaaatgtcg aaatttttcc aaatcagcgt cgcagcgggg gcatgcgcgt tgccggtttt
     1021 cctcagcgcc atgccggctc aggcgcagga ggggatcaaa accggctctc tggtctgcaa
     1081 cgtctcgggc ggcatcggct atctcgtggg ttcgcgcaaa gcggtgcatt gcacctttac
     1141 cggcacgaat gggcgttacg agcactatat cggccacatc accaaggtcg gtctggatat
     1201 cggcgcgacg accggcggcc agatcgtctg ggcggtgttc gagccgaccg cgcgccacgc
     1261 ttcgctcggc ggcaattacg ccggcgtgac cgcgcaggcg acgttcggcg ccgggctggg
     1321 cgcgaatgtg ctgatcggcg gctcgaacaa ggccgtcgcg ctgcagcccc tctccgtcga
     1381 aggccagacc ggcctcaacc tcgcggcggg cattggcggc cttcatctga cccgcacccg
     1441 ctgacgcttc cgccgcgccg ccggaaattc acgtgagcct cgcctcgccg gcagtgcgcg
     1501 gcggcgctta ttttcaaatc gctggccttt cgcgccagcc gaagatcagg ggagagctat
     1561 ggagtacagg caactcggcc attccggcct gaaagtctcc gccctcgcga tgggcacgat
     1621 gacgatgggc ggccgcggca atttttccaa ggtcggcagc accgggctcg acgaggcgcg
     1681 ccgccagatc gacctctgca tcgatgcggg cgtcaatctc atcgacacgg cgaatgtcta
     1741 ttccggcggc gcctcggagg aaatcatcgg cgaagccttg ggcggcaagc gcaagggcgg
     1801 cgtgctgatt gcgaccaagg cgcgctttcc gatgggcgac ggcccgaacg atcgcggctt
     1861 gtcgcggcac cacctcatcc gcgaatgcga agcgagcctg aagcggctgc gcaccgatgt
     1921 gatcgacctc taccaggtcc atcaatggga cggcctgacg ccgctcgaag agacgatgga
     1981 ggcgctcgac agcctcgtgc gcagcggcaa ggtaaggtat gtcggctgct cgaatttttc
     2041 cggctggcat gtgatgaagg cgatggagat cgcccgccgc gacgggcgcg ttccgttcgt
     2101 ctcgcagcag attcactata cgctgcaggc gcgcgaggcc gaatacgagc tcgtgccgat
     2161 ttcgatcgat cagggcctcg gcattcttgt ctggagcccg atcgccggcg gccttctctc
     2221 cggcaagcac cggcgcaacc gccaggcgcc ggaaggcacg cgccaattcg ccggctggaa
     2281 ggagccgccg atctatgacg aaggcaagct ctgggacatt gtcgacgcaa tcgtcgccat
     2341 cgccgattcg cgcggcgtct ccggcgcgca ggtctcgctc gcctggctgc tcggccggcc
     2401 gggcgtgacc tcggtcatta tcggcgggcg caccgaagcg cagttcaagg acaatctcgc
     2461 cgccgtcgag ctgaagctca cgccggacga gcgcaagcgt ctcgacgcgg tcagccggcc
     2521 gccgctgatc tatccttatt ggcaccagag ctggacggcg aaggaccggc tcggcgccgc
     2581 agacctcgcg ctgcacgcgg cctatctcga gaagtagaag aaacgtacct tatgtcgcgg
     2641 cgcgccccct ctctcgcttg cgggagaggg ctggggcgag ggtaacccgc aagtcataaa
     2701 tgagttcgcc tacagcgcct cgacaaaccg caccgccttg ccccgcgaag gcgtgacgat
     2761 ctcgccttcc cacatcacgc gctggccgcg gatgaccgtt ccgaccggcc agccggtcac
     2821 gttcttgccg tcgtacggcg tccagcggct gcgcgaggcg atccacgaat cgctgatcgt
     2881 cgcgcggcgc ttcaggtcga caatggtgaa atcggcgtcg tagccggcgg cgatccgccc
     2941 cttattggcg atgccgaaca agcgttgcgg gccggcgctg gtcaaatcga cgaagcgctg
     3001 cagggtcagc cgtccgttgg cgacatggtc cagcatgatc ggtacgagcg tctgtacgcc
     3061 ggtcatgccg gatgggctct caggataagg cttcgccttc tcttcgagcg tgtgcggcgc
     3121 gtgatccgag ccgagcacat cgatgatccc ttgttcgacg ccgcgccaga tggcgtcgcg
     3181 atgatgcgcc gcgcgcaccg gcgggttcat ctgaagcctg gttccgagcc gcgcgtaatc
     3241 ctcggcgctg aacgtcagat gttgcggcgt cgcctcgcag gtggcgacgt ccttgatgct
     3301 ttcgaggaag gcgatctctt cggcggtcgt gatgtgcagg acatggatgc gcgcgcgcgc
     3361 ctggcgcgcc agcgcgacca ggcgctgggt cgctttgagc gccgcgatct cgtcgcgcca
     3421 gaccggatgc gaagcggggt cgcccttcac ccgaagcggc ttgcgctcgt tgagcctttc
     3481 ctcgtcctcg ctgtggaagg cggcgcggcg gcggatgttc ttcaagatcg ccagcacgcc
     3541 ggcgtcgtcc ggcaccagca gcttgccggt cgaggcgccc atgaaaacct tgatccccgc
     3601 ggcgccgggc aggcgctcca aatccgtcag gtcgcggaca ttgtcatgcg tgccgccgac
     3661 ccagaaggcg aagtcgcaat gcatgcggct cctggcgcgg ccgagcttgt cctccagggc
     3721 cgcggcggag acggtcagcg gatcggtgtt cggcatttcg aagaccgcgg tcacgccgcc
     3781 gagcacggcg gcggccgagc cggtctccaa atcttcttta tgcgtcgcgc ccggctcgcg
     3841 aaaatggacc tggctgtcga tcacgccggg caggatatgc aggccgcggc aatcgatccg
     3901 ctcgccggcc gcggcctggc cgagcggccc gatcgcggcg atcctctcgc ccacgacgcc
     3961 gatgtccctc gcgccgacgc cgtcctggtt gacgacgatt ccgccttcga gaatgagatc
     4021 gaaacgcgcg ggcatgagac agcgcctcct cgcgccgcga aggcgcgctc aaatcgtacg
     4081 tcgtcattgc gagcgaagtg aagcaatcca
//
LOCUS       DATGJW010000189         5506 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_242590, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000189 DATGJW010000000
VERSION     DATGJW010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5506)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5506)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5506
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_242590"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VKU03_05740"
     CDS             <1..525
                     /locus_tag="VKU03_05740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012564059.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HLJ70799.1"
                     /translation="DDALAEILAAAVEQGIAADAAIAATLEQRKAFWRLREEMSDAQR
                     PEGGSIKHDVSVPVGATADFIAEASAAVLAYMPDARVVAFGHLGDGNIHFNVSQPVGA
                     DKTAFLGRWKEMNAVVHAVVARYRGSISAEHGVGQLKRDLLRQTKDRAALDAMRLIKA
                     ALDPNGVLNPGKVL"
     gene            593..1387
                     /locus_tag="VKU03_05745"
     CDS             593..1387
                     /locus_tag="VKU03_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264243.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HLJ70800.1"
                     /translation="MPLATRLRFWLFCGFLACSGTLAAAWLGAEPAIAQSAGPCSATA
                     YDGANYIVCSFDARRDDIRLFWKGPDNQAYGGFSRLASALAARGRALRFAMNAGMYET
                     NLTPVGLYIEDGRQRHRADLRDGAANFHLKPNGVFYIGDGAAGVVETSRFLADGAKAR
                     FATQSGPMLLIDGRMHPKISPTGASAKIRNGVGVRDNSLVFFVLSEEPVTFHAFAEFF
                     RDRLNCQNALYLDGSVSSVYAPNLDRREDPLEPIGPIVAVTAPSGG"
     gene            complement(1441..2304)
                     /locus_tag="VKU03_05750"
     CDS             complement(1441..2304)
                     /locus_tag="VKU03_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006797 - polyphosphate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PPK2 family polyphosphate kinase"
                     /protein_id="HLJ70801.1"
                     /translation="MDYRKEFLVKPGKRFKIAKADPAFLDAKLDKDEAQRVVEANRQR
                     IANGHTRLYAERRQSLLIVLQGLDTAGKDGVVTHLFRSVSPLGCVATSFKQPSAEEQA
                     HDFLWRAHKATPPRGFIAIFNRSYYEGVLVERVHDLIGKKELRQRYRQINDFERLLAE
                     TGTTIVKFFLNISPEEQLQRFKDRLDDPAKQWKISEADYKERDYWDDYIEAYEDALEE
                     CSADHAPWYVIPANHKWFRDLVASQIIADTFDEMHIAEPKPAVDLKRIRKLYEEDAKR
                     SPHSRREKVRE"
     gene            complement(2326..3585)
                     /locus_tag="VKU03_05755"
     CDS             complement(2326..3585)
                     /locus_tag="VKU03_05755"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008967155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HLJ70802.1"
                     /translation="MTIYTGPVFEMARTQFHVIADYLEIPADERDRLLLPKRAITVSC
                     PIHLDDGRTAVFQGYRVQHHLTLGPTKGGTRFAAKVDIGEIAALAIWMSWKCALAELP
                     YGGAKGGVTVDPSALSWKELENVSRRYMQEMIPFVGPRVDVMAPDMGTNEQIMAWFMD
                     TYSMYQGITVPEIVTGKPVDSGGTLGRREATGHGVAFLVFRAMEKLGLQPGQATAIVQ
                     GFGNVGSFAALGLAERGVRIVGLSDHTAAYYDPKGLDIQAVNRHVAAHRVLAGFSTEA
                     VIDPDALLEQRCDVLAPAAVERVITAANAGKLQCRILAEGANGPTTPEADLILNERRG
                     EVFIIPDILCNAGGVIVSYFEWVQDLQQYFWSKKEVMSRLEQALDRSWRNVVRRAERD
                     GVPNRVAAMAIGVERVRDAKQMRGLFP"
     gene            complement(3774..4862)
                     /gene="aroC"
                     /locus_tag="VKU03_05760"
     CDS             complement(3774..4862)
                     /gene="aroC"
                     /locus_tag="VKU03_05760"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HLJ70803.1"
                     /translation="MSHNSFGRLFQVTTFGESHGPAIGCVVDGCPPMIPLEAADIQAD
                     LDRRRPGTSRFTTQRREPDEVKILSGVFIDEKSGRQVTTGAPIGLIIENIDQRSKDYS
                     EIKDKFRPGHADFTYEAKYGLRDYRGGGRSSARETAMRVAAGAIARKIIPGVAVRGAL
                     VQIGAHKIDRSRWDWDEVGRNPFFCPDPVAAERFADYLDGVRKAGSSVGAVIEIVAEG
                     APAGWGAPIYGKLDAEIAGALMSVNAVKGVEIGAGFAAAALTGEENADEMRAGNDGRP
                     VFLSNHAGGILGGISTGQPIVARFAVKPTSTILSPRKTIDRFGAETEIATKGRHDPCV
                     GIRAVPVGEAMVACVLADAFLRHRGQIA"
     gene            5028..5252
                     /locus_tag="VKU03_05765"
     CDS             5028..5252
                     /locus_tag="VKU03_05765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006471729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70804.1"
                     /translation="MKKLLIALGLSSALLVTPALAQTDQNAAPAAPAADTAKAPMKAK
                     SHHHHKAKHVTKKSKKAKPAAPAEESAPKS"
BASE COUNT          912 a         1823 c         1798 g          973 t
ORIGIN      
        1 gacgacgcgc tcgccgaaat tctcgcggcg gcggtcgagc aaggaatcgc ggccgacgcc
       61 gcgatcgccg cgacgctcga acagcgcaag gcgttttggc gcctgcgcga ggagatgtcc
      121 gacgcccagc gcccggaggg cggctcgatc aagcacgatg tcagcgtgcc ggtcggcgcg
      181 acggcggatt tcatcgccga agccagcgcg gccgtcctcg cctatatgcc cgacgcgcgg
      241 gtcgtggctt tcggccatct cggcgacggc aacatccatt tcaacgtctc gcagccggtc
      301 ggcgccgaca agacggcgtt ccttggccga tggaaagaga tgaacgccgt ggtccacgcc
      361 gtcgtcgccc gctatcgcgg ctcgatctcg gccgagcacg gcgtcggcca gttgaagcgc
      421 gacctgctgc gccagactaa ggaccgggcg gcgctcgacg cgatgcggct gatcaaggcg
      481 gccttggatc cgaacggcgt cctcaatccg ggcaaggtgc tctgatccga tctttgctat
      541 gatggcgcgg agattcgttc tccgccgccg cgcgcctttc acgatgggac ctatgccgct
      601 cgcgaccagg ctccgatttt ggcttttctg cggctttctc gcctgcagcg ggacgctcgc
      661 cgccgcttgg cttggcgccg agccggccat tgcccaaagc gccggcccgt gctcagcgac
      721 ggcttatgac ggcgcgaact acatcgtctg ctcgttcgac gcgcggcgcg acgatatccg
      781 cctgttctgg aagggcccgg acaaccaggc ctatggcggc ttctcgcgtc tcgccagcgc
      841 gctcgccgcg cgcggccgcg ccttgcgctt cgcgatgaac gccggcatgt acgagaccaa
      901 cctgacgccg gtcggccttt acatcgagga cgggcgccag cgccaccgcg ccgatttgcg
      961 cgacggcgcc gccaatttcc atctcaagcc gaacggggtc ttctatatcg gcgacggcgc
     1021 cgccggcgtg gtcgagacgt cgcgctttct cgccgacggg gccaaggcgc gcttcgccac
     1081 ccaatccggg ccgatgctgc tgatcgacgg gcgcatgcat ccgaagattt cgccgaccgg
     1141 cgcttcagcc aaaatccgca acggcgtcgg cgtgcgcgac aattcgctgg tttttttcgt
     1201 gctctcggaa gagccggtga cgttccatgc cttcgccgaa ttcttccgcg accggctcaa
     1261 ttgccagaac gcgctctatc tcgacggatc ggtctccagc gtctacgcgc ccaatctcga
     1321 ccggcgcgaa gatcccttgg agccgatcgg cccgatcgtc gcggtgaccg cgccgtcggg
     1381 gggatagcgt tccttctccg gggtacggcg tcacaggaaa cattacgtac gccgtaccgc
     1441 tcactcccgt accttctccc ttcgggagtg gggtgagcgc tttgcgtcct cctcatagag
     1501 cttgcgaatc cgctttaggt cgaccgccgg tttcggctcg gcgatatgca tctcgtcgaa
     1561 cgtgtcggcg atgatttgcg atgcgacgag gtcgcggaac catttgtgat tggccgggat
     1621 gacgtaccac ggcgcgtggt cggcgctgca ttcttcgagc gcgtcctcgt aggcctcgat
     1681 atagtcgtcc caataatcgc gctccttgta gtccgcttcg ctgatcttcc attgcttggc
     1741 cggatcgtcg agccgatcct tgaagcgctg gagctgctcc tccggcgaga tgttcaagaa
     1801 gaacttgacg atcgtcgttc ccgtttcggc caagaggcgc tcgaaatcgt tgatctggcg
     1861 atagcgctgg cgaagctcct tcttgccgat cagatcgtgc acgcgctcga ccagcacgcc
     1921 ctcgtaatag gagcggttga aaatggcgat gaagccgcgc ggcggcgtcg ccttgtgggc
     1981 gcgccagagg aagtcatgcg cctgctcctc ggccgatggt tgcttgaacg aggtcgcgac
     2041 gcagcccagc ggactgacag agcggaagag atgggtgacg acgccgtcct ttcccgcggt
     2101 atcaagccct tgcagcacga tcagcagcga ttgacgccgt tcggcgtaaa ggcgcgtatg
     2161 cccattggcg atgcgctggc gattggcctc gacgacgcgc tgcgcctcgt ccttgtcgag
     2221 cttggcgtcg agaaaagccg gatcggcctt ggcgatcttg aagcgcttgc ccggcttgac
     2281 gagaaattcc ttgcgatagt ccatgcgcgc ccctttcggc tcggcttacg ggaacaggcc
     2341 gcgcatctgc ttggcgtcgc gcacgcgctc gacgccgatc gccatcgccg cgacccggtt
     2401 aggaacgccg tcgcgctcgg cgcggcgaac gacattgcgc catgagcggt cgagcgcctg
     2461 ttcgagccgg ctcatcactt ccttcttcga ccagaaatat tgctgcaaat cctgcaccca
     2521 ctcgaaatag ctgacgatga cgccgccggc gttgcacaga atatcgggga tgatgaagac
     2581 ctcgccgcgg cgctcgttga ggatcaggtc ggcttccggc gtcgtcggcc cgttcgcgcc
     2641 ctcggcgaga atgcggcact gcagcttgcc ggcattggcc gcggtgatca cccgctcgac
     2701 ggcggcgggc gcgagaacgt cgcagcgctg ctccagcaag gcgtccgggt cgatgaccgc
     2761 ttccgtggag aagccggcaa ggactcgatg ggcggcgacg tggcggttga ccgcctggat
     2821 gtcgaggccc ttcggatcgt aataggccgc cgtgtgatcg gaaagcccga caatgcggac
     2881 gccgcgctcg gcgaggccga gagcggcgaa ggagccgaca ttgccgaagc cctgaacgat
     2941 cgccgtcgcc tggcccggct gcagcccgag cttttccatg gcgcggaaga cgagaaaggc
     3001 gacgccgtgg ccggtcgcct cccggcgccc gagcgtgccg ccggaatcga ccggcttgcc
     3061 ggtgacgatt tccggcaccg ttatgccttg gtacatggag taagtgtcca tgaaccaggc
     3121 catgatctgt tcgttcgtgc ccatatcggg cgccatgacg tcgacgcgcg ggccgacaaa
     3181 gggaatcatt tcctgcatgt agcggcgcga gacgttctcc agctccttcc acgacagcgc
     3241 gctcggatcg acggtcacgc cgcccttggc gccgccataa ggcaattcgg cgagcgcgca
     3301 tttccaactc atccagatcg ccaaagcggc gatttcgccg atatcgacct tggcggcaaa
     3361 acgcgtgccg cccttggtcg gcccgagcgt cagatgatgc tggacgcgat agccctggaa
     3421 cacggcggtg cggccgtcgt cgagatggat cggacaggag acggtgatcg cgcgcttcgg
     3481 caggagcagg cggtcgcgct cgtcggcggg aatttcaaga tagtcggcga tgacgtgaaa
     3541 ctgcgtgcgc gccatctcga agacgggacc cgtatagatc gtcatcctag cgcttcctcc
     3601 gatctctcga tgagcccaga gtaggcggga cggttttggc aagcaaatct aaaacgcggg
     3661 aagggcggcg cgggtttcat cggccgccat gctgcagccg cggtcggcga ttcgcggcaa
     3721 tccctactcg cgcttgcggc attcggctcc gccgcgctga ggtacgaaaa ccgttacgcg
     3781 atctggccgc gatggcgcag gaacgcgtcg gcgaggacgc aggcgaccat cgcttcgccg
     3841 accggcacgg cgcggatgcc gacgcaaggg tcgtggcggc ccttggtggc gatctcggtc
     3901 tccgcgccaa aacggtcgat cgtcttgcgc ggcgacagga tcgtcgaggt cggcttgacg
     3961 gcgaagcgcg cgacgatcgg ctgcccggtc gagatgccgc ccaatatgcc gccggcatgg
     4021 ttcgacagga acaccgggcg gccgtcattg ccggcgcgca tctcgtcggc gttctcctcg
     4081 ccggtcaaag ccgcggcggc gaagccggcg ccaatctcga cgcccttgac cgcgttgacg
     4141 ctcatcagcg cgccggcgat ctcggcgtcg agcttgccgt agatcggcgc gccccagccg
     4201 gccggcgcgc cctcggcgac gatctcgatc accgcgccga ccgaggagcc cgccttgcgc
     4261 acgccgtcaa gataatcggc gaacctctcc gccgcgaccg ggtcgggaca gaagaaagga
     4321 ttgcggccga cttcatccca atcccaccgg cttcgatcga ttttgtgcgc gccgatttgc
     4381 acgagcgcgc cgcgcacggc gacgccggga ataatcttgc gcgcgatcgc gccggcggcg
     4441 acccgcatcg ccgtctcgcg cgccgaggag cggccgccgc cgcgatagtc gcgcaacccg
     4501 tatttcgcct cgtaggtgaa atcggcatgg cccggccgga acttgtcttt gatttcgctg
     4561 tagtctttcg agcgctgatc gatgttttca atgatcaggc cgatcggcgc gccggtcgtg
     4621 acctggcggc cggatttctc gtcgatgaaa acgccggaaa gaatcttcac ctcgtccggc
     4681 tcgcgccgct gcgtggtgaa gcgcgaggtt ccgggccggc gccggtcgag atcggcctgg
     4741 atgtcggccg cctctagcgg gatcatcggc ggacagccgt cgacgacgca gccgatcgcc
     4801 ggcccatggc tctcgccgaa ggtcgtcact tgaaagagac gtccgaaact attgtgcgac
     4861 atgagcaatc ctcgactgct gtttagaagc gcggcggtcg agcgtcaaac cgcggcgccg
     4921 cgcggcgcgg ccggtctcgg cgccgttcat ccaaaattca ggtgaatttt gggatgagtc
     4981 gcgcgcctga gacgcttccg tcccttggct gaacacggag aaacaacttg aaaaagctgc
     5041 ttatcgcgct cgggctcagc tcggcgctcc tcgtcacccc ggctttggcc cagaccgacc
     5101 agaacgccgc tccggccgcc ccggccgccg acacggcgaa ggcgccgatg aaggcgaagt
     5161 ctcatcatca tcacaaagcc aagcacgtga cgaagaagag caagaaggcc aagcctgccg
     5221 cgccggccga ggagagcgcg ccgaagagct gatttcgctc cccggcgcga cggttggagc
     5281 gcgccgcgac ctgatcaggc cgcgcccctc gccttgcgga gggcgcggtt ctttttttgg
     5341 aattggcggc tcgacgcctc agccgatcca ggtcgcctcg cccatgtcga agacgagcgc
     5401 tcgtccctgc cagatcggcg cgctggccgc ctcggcgatc ggcattccgg ccagcagcgc
     5461 caggaaggcg cgcgccaccc cgccatgcgc gacgaggagc gcgtcg
//
LOCUS       DATGJW010000190         7903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_657866, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000190 DATGJW010000000
VERSION     DATGJW010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7903
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_657866"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(155..673)
                     /locus_tag="VKU03_05770"
     CDS             complement(155..673)
                     /locus_tag="VKU03_05770"
                     /EC_number="3.4.23.43"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prepilin peptidase"
                     /protein_id="HLJ70805.1"
                     /translation="MIENLLLLVTLVLFPALMAFSASSDIFSMKISNRVSLALAAGYP
                     VLAVAVGVPAAAILTNIGCGAAVLVLTFFMFTRGWIGGGDAKLAAATAPWLGWALMLD
                     YGLIASVLGGLLTLAIVLARRAPLPAWAERLDWVARLHHPGNGVPYGVALAAAGLALY
                     PSSQIWRAATGL"
     gene            complement(849..1013)
                     /locus_tag="VKU03_05775"
     CDS             complement(849..1013)
                     /locus_tag="VKU03_05775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009798338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp family type IVb pilin"
                     /protein_id="HLJ70806.1"
                     /translation="MIKSLKSFLANESGATAIEYGLIASLIAVTIITAITVVGTKLQT
                     TFNEVASNLH"
     gene            1252..2175
                     /locus_tag="VKU03_05780"
     CDS             1252..2175
                     /locus_tag="VKU03_05780"
                     /inference="COORDINATES: protein motif:HMM:NF016894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxygalactonokinase"
                     /protein_id="HLJ70807.1"
                     /translation="MPKDSPTLIAVDWGTTSLRAYLLGGRGVVLDRLEAARGVQNVGK
                     GEHEAVLAGMIGAWRQNEGPPILLSGMVGSRQGWVEAPYVRTPAGLGETAAAIVTIDS
                     KTLGAIGVVPGVLTEGAALGPDVMRGEETQIFGALAASGRADGLFVLPGTHSKWARAE
                     KGRIVWFATYMTGEAFAALREHTILARLMAAGGEDPDGFAAGVAAAKALAAPGDLLHA
                     IFMTRTLGLFDRLAPAQLPEYLSGLLIGAEILAAAQGGGEAATVIGSPALTRRYCEAG
                     KTLGLRFGAAPDGCVALGQQALFAAWRGSAD"
     gene            complement(2164..5835)
                     /gene="putA"
                     /locus_tag="VKU03_05785"
     CDS             complement(2164..5835)
                     /gene="putA"
                     /locus_tag="VKU03_05785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0003842 - 1-pyrroline-5-carboxylate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0004657 - proline dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trifunctional transcriptional regulator/proline
                     dehydrogenase/L-glutamate gamma-semialdehyde
                     dehydrogenase"
                     /protein_id="HLJ70808.1"
                     /translation="MTTAAPLSASPKAPDAPFVAFGAAIVPHAGLRAAIDAAYRRPER
                     ECLPALIEEATLAPTQRRAAQDLARDLVEKLRARPTPGLVQGLMREYALSSQEGVALM
                     CLAEALLRIPDSATRDALIADKVGHGEWRAHVGHSPSPFVNAATWGLLITGKLVGTFD
                     EAGLATALTRLIARSGAPIIRASVDTAMRLMGEQFVCGETIEEALRNSRRLEALGFRY
                     SYDMLGEAAATMADADRYLAAYAEALHAIGAASAGRGIYEGPGLSIKLSALHPRYSRP
                     QRERAMSELLPRLKSLVALARQYDVGINIDAEEADRLDLSLDLLEALCADPDLAGWNG
                     IGFVVQAYQKRAEAVIAFVIDLARRTKRRIMLRLVKGAYWDSEIKRAQVDGLEDFPVF
                     TRKIHTDVSYLACARLLLAAPDAVYPQFATHNALTLATIQAMAGDNYYAGQYEFQCLH
                     GMGEPLYLQVVGAGRLGRPCRVYAPVGSHETLLAYLVRRLLENGANTSFVNRIADANV
                     PTDALLEDPVEAAREVAPLGAPHPKIALPGEIFAPERVNSSGIDLSNETRLAALAIAL
                     KDSAAKTWRAGGPGGAKRLIVNPADRRDMVGEAYHASAGDAAAAVEAAHAAWPRWRAR
                     SPAERAALLERAAALYEQNLGALVGLMCREAGKTVPNAIGDVREAVDFLRYYAARVKE
                     DFANETHRPLGVVACISPWNFPLAIFTGQVAAALAAGNCVVAKPAEETPLVAAEGVRL
                     LHQAGVPAEALVLVAGEGDVGAALTAHPLVAGIMFTGSTEVARHIQRALAERLGPDGA
                     PIPLIAETGGQNALIVDSSALAEQVVADALASAFDSAGQRCSAARILCLQADIADRTL
                     AMIKAAMIELKVGRPDRLSTDIGPVISAEAQQRLNDHVAAMRRRGFPVYAPTLPEECA
                     AGLFVAPTLIEISKVADLSREVFGPVLHVLRYQRDALPQLLDEINASGYGLAGGAHSR
                     IDATIALVSDGLSAGNIYINRNIIGAVVGSQPFGGHGLSGTGPKAGGPLYLKRLLAAA
                     PPDWLRLGPSSPPALRELAAWLDGSGRQSLARRCEELARRSRLGAERDMPGPVGERNR
                     YRLRPRGVALCHAAGEEAAILQFACALAAGDRAALAGAGGAKLFAQLPGRLRKEAILA
                     DEKTGYDVVMTDAEGAALLALLQQVAAREGPIAGVHALSRERLDRGDLWPLDFLLNEQ
                     AVATNTTAAGGNASLMSIG"
     gene            6063..6368
                     /locus_tag="VKU03_05790"
     CDS             6063..6368
                     /locus_tag="VKU03_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70809.1"
                     /translation="MGKTWMTKWGPRKVREIPPTLEEAFVAAECLTDDLNEKIEIAAS
                     LMGMPVEDVRAQAAKLSGQAQRPPVTTGRGRPVVVQYKRPRLGARPPTPPSPFARRG"
     gene            6455..>7903
                     /locus_tag="VKU03_05795"
     CDS             6455..>7903
                     /locus_tag="VKU03_05795"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70810.1"
                     /translation="MLDFPVQRSPAGALAILARLQRDYGWPHKWAYASGALLLALVAG
                     ATATSAWLLKPVLNGMAGAERFADMRPLAFAVLGLFVLRGLATYGALVILSRAGNRII
                     ATAQRRVFDHLLRQNMLFFQDRHSSEFVARLALAANGVRDALQFVIQGVARDALMVAS
                     LIAVMALRDPLLTVLALCVLPVAILLLRRVIRRVRSFARRSFDGSTSIMQTMSETVLG
                     MRIVKAFNLEETMRRRMSGAIKEVERSANRISAGGALAVLMADSLAGLAIGFAIYYGS
                     WRISVHHGDVGSFVSFLAALLMAYEPIKRLGRLNVDIQNGLVGARLIYEVLDAPMAEG
                     PEALRPKLAIGAGRLMIEKARFGFRPGEWAIDGLDLVAEPNMTTALVGPSGGGKSTIL
                     GLIQRFYALQEGRILIDGVDIAAVELGSLRDKIAFVSQDVFLFRGSIRDNIALGRPDA
                     SDAEIVEAARKAHAHDFILQFGAGYDTNVGEHG"
BASE COUNT         1269 a         2663 c         2716 g         1255 t
ORIGIN      
        1 gctttgttca taacgccaaa ccctcaccgc gcccctgacg ccgacgcgcc tttgcacgcc
       61 gatttggccc tatttgaacc gtcaatcgtc aaattagggt taaccagacg tatctaatcc
      121 gcccaggcgg ggctcccggg agtgcttcgg cgccttagag accggtcgcg gcgcgccaaa
      181 tctggctgct cggatagaga gccaagcccg ccgccgcgag ggcgacgcca taggggacgc
      241 cgtttcccgg gtgatgcagc cgggcgaccc agtcgaggcg ctccgcccag gccggcagcg
      301 gcgcccgccg cgccagcacg atggccaaag tgagcaggcc gccgaggacc gaggcgatga
      361 ggccgtagtc gagcatcagc gcccagccca gccagggcgc ggtcgccgcg gcgagcttgg
      421 cgtcgccgcc gccgatccag ccgcgcgtga acatgaagaa ggtcaggacc agcacggccg
      481 cgccgcagcc gatattggtc aaaatcgccg cggccgggac gccgacggcg acagccagaa
      541 ccggatagcc ggcggcgagg gcgagactga cgcggttgga tattttcatc gagaaaatat
      601 cggacgacgc cgagaacgcc atcagcgccg ggaaaaggac cagggttaca aggagaagaa
      661 ggttttcgat catggtccgc cggccgcccg gtcttttcgc agcaaggaca ataggagcga
      721 ggggttgagg tcaggttacc gagcatcgat aaaacccgcc gagcgccgca cgatcacccc
      781 gaaatgcaaa gcgcgccgac gagcctcgct cgccggcgcg caagaaatcg tatcgagcgc
      841 ccgccggatc agtgcaggtt gcttgcgact tcgttgaacg tcgtctgcag cttggtgccg
      901 accacggtga tggcggtgat gatcgtgacg gcgatcagcg aggcgatcag gccgtattcg
      961 atagcggtgg cgccggattc gttggcgagg aagctcttga gggacttgat catggggttt
     1021 gctccgtgct ccagttttct cactccggca aagctctccg tctcctgctc gtcggaactg
     1081 gcgcacgcta tcgtccgcgc cgtttcaaac gcgttaactc gaccgcatgc aatgggacct
     1141 gttggggaat acccgcaaat tggttaagcc ccgcctaacc cgtcagctta tttgccgcca
     1201 atgcggcccg gccgccgccg gcgccgctgc aattcgaggg aaaggtcaat cttgccaaag
     1261 gactcgccga cgctgatcgc ggtcgattgg ggcacgacgt cgctgcgcgc ctatttgctc
     1321 ggcggccgcg gcgtcgttct cgaccgtctc gaagcggcgc gcggcgttca gaatgtcggc
     1381 aaaggcgagc acgaagccgt gctcgccggc atgatcgggg cctggcgcca aaacgaaggg
     1441 ccgccgattc ttctttccgg aatggtcggc tcgcgccagg gctgggtcga ggcgccctac
     1501 gtgcgcacgc cagccggtct cggcgagacc gccgcggcga tcgtgacgat cgacagcaag
     1561 acgctcggcg cgatcggcgt cgttcccggc gtgctcaccg aaggggcggc gctcggaccg
     1621 gacgtcatgc gcggcgagga gacgcagatt ttcggcgcgc tcgccgcatc cggccgcgcc
     1681 gacgggctct tcgtcctgcc gggcacgcat tcgaaatggg cgcgcgccga aaaaggccgg
     1741 atcgtctggt tcgccaccta catgaccggc gaagcgttcg ccgccttgcg cgagcacacg
     1801 atcctggcgc ggctgatggc ggcgggcggg gaggaccccg acggcttcgc cgccggcgtc
     1861 gccgccgcca aggcgctggc cgcgcccggc gaccttctcc atgcgatttt catgacccgg
     1921 acgctcggcc tgttcgaccg tctcgcgccg gcgcaattgc cggaatacct gtccggcctg
     1981 ctgatcggcg ccgagattct cgccgccgcg caaggcggcg gcgaggcggc gacggtgatc
     2041 ggctcgccgg cgctgacccg ccgctattgc gaggcgggaa agactctcgg cctgcgcttc
     2101 ggcgcggcgc cggacggctg cgtcgcgctc ggccagcagg cgctgttcgc cgcctggcgc
     2161 ggctcagccg attgacatca ggctggcgtt gccgcccgcc gccgtcgtgt tggtcgcgac
     2221 ggcttgttcg ttcaacagga agtccaaggg ccagaggtcg ccgcggtcga gccgctcgcg
     2281 cgacagcgcg tggacaccgg cgatcggccc ttcccgcgcc gcgacctgct gcaagagagc
     2341 gagcagcgcc gccccttcgg cgtcggtcat gacgacgtcg taacccgtct tttcgtcggc
     2401 gaggatcgcc tccttgcgca gccgcccggg caattgcgcg aagagcttgg cgccgcccgc
     2461 gccggcgagc gccgcgcgat cgccggcggc gagcgcgcaa gcgaattgca ggatcgccgc
     2521 ttcttcgccg gccgcgtgac aaagcgcgac gccgcgcggg cgcagccgat agcgattgcg
     2581 ctctccgacc gggccgggca tgtcccgctc ggcgccgagc cgcgaacggc gagcgagttc
     2641 ttcgcagcgg cgcgccaggc tctgtcgtcc tgacccatcg agccacgcag ccaattcgcg
     2701 cagagcgggc ggcgaggatg ggccaaggcg cagccaatcg ggcggcgcgg cggcgagcag
     2761 gcgcttgaga tagagcggcc cgccggcttt cggcccggtg ccggaaaggc catggccgcc
     2821 gaagggctgc gagccgacca ccgcgccgat aatgttgcgg ttgatataga tattgccggc
     2881 cgagaggccg tcgctgacca gcgcgatcgt cgcgtcgatg cgcgaatggg cgccgccggc
     2941 aaggccgtag ccggaggcgt tgatctcatc gagcaattgc ggcagcgcgt cgcgctgata
     3001 gcgcagcacg tgcagaacag ggccgaacac ttcgcgggaa aggtcggcga ctttgctgat
     3061 ctcgatcaaa gtcggcgcga cgaacaggcc cgctgcgcat tcttcgggaa gcgtcggcgc
     3121 gtaaaccgga aagccgcgcc gccgcatcgc cgccacatgg tcgttgaggc gctgctgcgc
     3181 ctccgccgag atgaccgggc cgatatcggt cgacaggcga tcggggcggc cgactttcag
     3241 ctcgatcatc gccgccttga tcatggcgag ggtacggtcg gcgatgtccg cctgtaggca
     3301 cagaatacgc gccgccgagc agcgctgtcc cgccgaatcg aaggccgaag ccagcgcgtc
     3361 ggcgacgacc tgctcggcca gcgccgacga atcgacgatc agcgcattct ggccgccggt
     3421 ctcggcgatc agcgggatcg gcgcgccgtc ggggccgagc ctttcggcga gggcgcgctg
     3481 gatatggcgc gccacctcgg tcgagccggt gaacatgatt ccggcgacca acggatgcgc
     3541 cgtcaaagcg gcgccgacat cgccctcgcc cgcgaccaga acaagcgctt cggccggaac
     3601 ccccgcctga tgcagcaagc gtacgccctc ggcggcgacg agcggcgtct cctcggccgg
     3661 cttggcgacg acgcaattgc cggcggcgag cgccgccgcg acctgaccgg tgaaaatcgc
     3721 cagcgggaaa ttccatgggc tgatgcaggc gacgacgcca agcgggcgat gggtctcgtt
     3781 ggcgaaatcc tctttgacgc gggccgcata gtagcggagg aaatcgaccg cctcgcgcac
     3841 gtcgccgatc gcgttgggca cggttttgcc cgcttcgcga cacatcagcc cgaccagcgc
     3901 gccgagattc tgctcataga gcgccgcggc gcgctccagc aaggcggcgc gctcggcggg
     3961 agaacgggcg cgccagcgcg gccatgccgc atgcgccgcc tcgaccgccg cggcggcgtc
     4021 gccggcgctc gcatgatagg cttcgccgac catgtcgcgg cggtcggccg gattgacgat
     4081 caggcgcttt gcgccgcccg gaccgccggc gcgccaagtc ttggctgcgc tgtctttgag
     4141 ggcgatggcg agcgccgcca ggcgggtttc gttgctgaga tcgattcccg aggaattgac
     4201 ccgctccggc gcgaagattt cgccgggcaa ggcgattttc ggatgcggcg cgccgagcgg
     4261 cgcgacctcg cgcgccgcct cgaccgggtc ttccagcaag gcgtcggtcg gaacgttggc
     4321 gtcggcgatc cggttgacga aggacgtatt ggcgccgttc tcgagcagcc ggcgcacgag
     4381 ataagcgagc agcgtctcat gcgagccgac cggcgcatag acccggcacg gccggccgag
     4441 ccggccggcc ccgacaacct ggaggtagag cggctcgccc attccgtgca ggcattggaa
     4501 ttcgtactgg ccggcatagt agttatcgcc ggccatggcc tggattgtcg ccaaggtcag
     4561 cgcgttgtgg gtggcgaatt gcggatagac cgcgtccggc gcggcgagca aaagccgcgc
     4621 gcaagcgagg tacgacacat cggtatggat cttgcgggtg aagaccggga agtcttcgag
     4681 gccgtcgacc tgcgcgcgct tgatctcgct gtcccaataa gcgcctttga ccaggcgcag
     4741 catgatccgc cgcttggtgc ggcgggcgag atcgatgacg aaggcgatga ccgcttcggc
     4801 gcgcttctgg taggcctgga cgacgaagcc gattccattc cagccggcaa ggtcggggtc
     4861 ggcgcagagc gcctccaaga gatcgagcga aagatcgagc cggtccgctt cctcggcgtc
     4921 gatattgatg ccgacatcgt actgtcgagc cagagcgacg aggcttttaa ggcgcggcag
     4981 caattcgctc atcgcccgct cgcgctgcgg gcgtgaatag cgcggatgca gggcggaaag
     5041 cttgatcgag aggcccggcc cttcatagat gccgcgcccc gccgaagcgg ccccgatcgc
     5101 gtgcagcgcc tcggcatagg cggcaagata gcgatcggcg tcggccatcg tggcggccgc
     5161 ttcgccgagc atgtcgtagg aatagcggaa gccgagcgct tcgaggcggc gggaattgcg
     5221 caacgcctcc tcgatcgttt cgccgcagac gaattgctcg cccatcaggc gcatcgcggt
     5281 gtcgacgctg gcgcggatga tcggcgcgcc gctgcgcgcg atcagccggg tcagcgccgt
     5341 cgcgaggccg gcttcgtcga acgtgccgac cagtttgccg gtgatcagca ggccccaggt
     5401 cgcggcattg acgaaaggcg aaggcgaatg gccgacatgg gcgcgccact cgccatggcc
     5461 gactttgtcg gcgatcagcg cgtcgcgcgt cgcgctgtcg ggaatgcgca gcaacgcctc
     5521 ggccagacac atcagcgcga cgccttcctg gctcgacagc gcatattcgc gcatcaggcc
     5581 ctggacgagg cccggcgtcg gccgagcgcg cagcttctcc acaaggtctc gcgcgaggtc
     5641 ttgggcggcg cggcgctggg tcggcgcgag ggtcgcctcc tcaatcaagg ccggcaggca
     5701 ctcgcgctca ggccgtcgat aggcggcgtc gatcgccgcg cgcaatccgg cgtgcgggac
     5761 gatcgccgcg ccgaaagcga cgaacggcgc gtccggcgcc tttgggcttg cagagagggg
     5821 cgcggccgtc gtcattggcg ggaactctca gggaggaaca tttcaggacg cgtcgcattg
     5881 tcgcgacgcc tagcgccctt atgccacggc gcctagatgg aatggagagg tcaggcgcgc
     5941 gcgggggcgg cgttctgata ttgcagggca caaaattatt gctgcggtgc agcatgcgtg
     6001 ctaagcttca tcctggaacg atgactttgc gctatgatcg gcgccgctga ttcgaggacg
     6061 ccatgggcaa gacttggatg acgaaatggg ggccgcgcaa agtgcgcgaa atcccgccga
     6121 cgctcgaaga ggctttcgtc gccgccgaat gcctcaccga cgacttgaac gaaaagatcg
     6181 aaatcgccgc ctcgctcatg ggcatgccgg ttgaagacgt gcgcgcccag gcggccaagc
     6241 tttccggcca ggcgcagcgg ccgccggtga cgaccggccg aggccgcccc gtcgtcgtgc
     6301 aatacaagcg gccgcgcctc ggcgcccggc cgccgacgcc gccctccccc ttcgcccggc
     6361 gcggctgaac ctgtccacga cggatcgaca gcggcgcacc gcgccggcgc attctaagct
     6421 tcccccggcc gccgcttcgc tttagcaaga gcggatgctc gattttcccg ttcagcgctc
     6481 gccggctggc gcgctcgcca ttctcgcgcg gctgcagcgc gactatggct ggccgcataa
     6541 atgggcttat gcgagcggcg cgctgctgct cgccctggtc gccggcgcga ccgcgacctc
     6601 agcctggctg ctcaagccgg ttctcaacgg catggctggg gcggagcgct tcgccgatat
     6661 gcggcccctc gccttcgccg tgctcggcct gttcgtgctg cgcgggcttg cgacctatgg
     6721 cgcgctggtc attctctccc gcgccggcaa ccggatcatc gccacggcgc agcgccgcgt
     6781 cttcgatcac ctgctgcggc agaacatgct gttcttccag gaccgccatt cgagcgaatt
     6841 cgtcgcccgc ctggcgctcg ccgccaatgg cgtgcgcgac gcgctgcaat tcgtcatcca
     6901 gggcgtggcg cgcgacgcgc tgatggtcgc ttcgctgatc gcggtgatgg cgctgcgcga
     6961 tccgcttctg accgtgctgg cgctctgcgt tctgccggtc gcgattctgc tcctccgccg
     7021 ggtcattcgc cgcgtccgct ccttcgcccg ccgctccttc gacggctcga ccagcatcat
     7081 gcagacgatg agcgaaaccg tgctcggcat gcgcatcgtc aaggccttca acctcgaaga
     7141 gacgatgcgc cggcgcatga gcggcgcgat caaggaggtc gagcgctcgg ccaaccgcat
     7201 ttccgccggc ggcgcgctcg ccgtgctgat ggccgattcg ctcgccggtc tcgccatcgg
     7261 cttcgccatc tattacggct cgtggcggat cagcgttcat catggcgacg tcggctcgtt
     7321 cgtctccttc ctcgccgcgc tgctgatggc ctatgagccg atcaagcggc tcggccggct
     7381 caatgtcgac atccagaacg ggctggtcgg cgcgcgcctg atctatgagg tgctcgacgc
     7441 gcccatggcg gagggacccg aggcgcttcg gcccaagctc gccattgggg ctggccggct
     7501 catgatcgaa aaggcgcgct tcggcttccg gcccggcgaa tgggcgatcg acgggctcga
     7561 cctcgtcgcc gagccgaaca tgaccactgc cctggtcggc ccgtcgggcg gcggcaaatc
     7621 caccattctc ggcctgatcc agcgcttcta cgccttgcag gaaggacgca ttctcatcga
     7681 cggcgtcgac atcgccgcgg tcgagctcgg ctcgctgcgc gacaagatcg ccttcgtgtc
     7741 gcaggacgtg ttcctgttcc gaggctcgat cagggacaat atcgcgctcg gccggcccga
     7801 cgcctccgac gccgagatcg tcgaggcggc gcgcaaagcg catgcgcacg atttcatctt
     7861 gcagttcggc gccggctacg acaccaatgt cggcgagcac ggc
//
LOCUS       DATGJW010000191        10252 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1072734, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000191 DATGJW010000000
VERSION     DATGJW010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10252)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10252)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10252
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1072734"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /gene="hpaR"
                     /locus_tag="VKU03_05800"
     CDS             <1..400
                     /gene="hpaR"
                     /locus_tag="VKU03_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013528542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="homoprotocatechuate degradation operon regulator
                     HpaR"
                     /protein_id="HLJ70811.1"
                     /translation="PMLAQHGVTEQQWRVIRVLSEEGPLGATEVADRAAILGPSLTRI
                     IKTLERRRMITSRRLPHDGRRVVLEIAAAGRAVIASAAPERRKIYRDLERRHGQEALD
                     DLLDRLEALILREQKGAGDGKRRKERAQKG"
     gene            complement(442..1314)
                     /locus_tag="VKU03_05805"
     CDS             complement(442..1314)
                     /locus_tag="VKU03_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205070.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLJ70812.1"
                     /translation="MRIVSFTAANRNSFGLLNERGVVDVGRRLKDAYRGLDDLLTREG
                     GEGARQRLEACAGDPADYALGEIALQKPLLDWGKCFCVGVNYPERNAEYKDNSEQPKY
                     PSLFVRFPASFVGPGEALVRPPESRELDYEGEIVLVIGKAGRRIRAADWTGHVFGYLL
                     ANEGTIRDWVRHGKFNVTPGKNWAASGALGPWITTLDEAGPGPFTLVTRVNGEVRQSD
                     STERMMFPFGRIVEYISTFCPLAPGDIILTGTPSGAGARRDPPVWLRPGDEVEVEATG
                     LGSLANGVRDETGA"
     gene            complement(1341..2141)
                     /gene="hpaH"
                     /locus_tag="VKU03_05810"
     CDS             complement(1341..2141)
                     /gene="hpaH"
                     /locus_tag="VKU03_05810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004687628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018817 - 2-oxo-hept-3-ene-1,7-dioate
                     hydratase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo-hepta-3-ene-1,7-dioic acid hydratase"
                     /protein_id="HLJ70813.1"
                     /translation="MGPDDHIAAAKRLDAAERERKQIRMLTLDHPAMTMADSYAIQKA
                     WLDIKLKRGGRIVGHKIGLTSKAMQAAVGVKEPDSGFLLDDMVFADGGEIPSGRFIGL
                     RVEAELAFVLGKPLAGPNCTASDAVAASDFVSPALELLDTRIFRVDPETGKTRTVRDT
                     IADNAANAGLILGGRRFRPFDHDLRWIGAICAKNGEVEETGLAAGVLNNPATGIAWLA
                     NRLAENGERLEAGEIVLAGSFIRPIEVGRGDRIHADFGAFGAVSCHFG"
     gene            complement(2153..3133)
                     /gene="hpaD"
                     /locus_tag="VKU03_05815"
     CDS             complement(2153..3133)
                     /gene="hpaD"
                     /locus_tag="VKU03_05815"
                     /EC_number="1.13.11.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008687 - 3,4-dihydroxyphenylacetate
                     2,3-dioxygenase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxyphenylacetate 2,3-dioxygenase"
                     /protein_id="HLJ70814.1"
                     /translation="MAIPAHVLYPPFNIVRLSHASLTVSDLAASRAFYVDTLGLQATS
                     EDESRIYLRAMEERCHHSLILEKGDVGLAGPLAFKVFDEDHLDRAKSFFDQRGFPTRW
                     VETPYQGRTFRTRDNVGIPLEFYVSMERLPPIHQKYALYRGVKPLRIDHFNCFSPDVR
                     ASAEFYASIGFRMTEYTDTDDGAELWAAWMHRKGGVHDVAFTNGLGPRLHHVAFWVPT
                     PLNIIDLLDLMATTGYLGNIERGPGRHGISNAFFLYVRDPDGHRVEIYCSDYQTVDPD
                     LEPIRWGLSDPQRQTLWGAPAPRSWFEEGSVFAGVEPVRPKLNATPIVAP"
     gene            complement(3146..4696)
                     /gene="hpaE"
                     /locus_tag="VKU03_05820"
     CDS             complement(3146..4696)
                     /gene="hpaE"
                     /locus_tag="VKU03_05820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018480 -
                     5-carboxymethyl-2-hydroxymuconic-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:1901023 - 4-hydroxyphenylacetate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate semialdehyde
                     dehydrogenase"
                     /protein_id="HLJ70815.1"
                     /translation="MNQASILTSTLADNQERAEACLRRFAGAVVPHFIAGAPALSQSG
                     ATFETLDPATNRRQCLVASGGPAEVERAAAAAAAAFKTWRNVPGAKRRAILHAIADAI
                     EARADEIALIESSDTGQPIRYMAKAALRGAENFRFYADRAPGAGDGLALPDAAHINYT
                     MRQPIGPVGVITPWNTPFMLSTWKIAPALAAGCTVVHKPAEWSPLSAVLLTEIMDETI
                     RAHGGPAGVVNLVQGLGETAGKALTEHDAIKAIAFVGETVTGSQIMRQGAATLKRVHF
                     ELGGKNPVMIFEDADQERALDAALFMIYSLNGERCTSSSRALVQRSIYDSFVARAAKR
                     VAAIKVGHPLDPATEIGPLIHPRHAEKVLSYAALAREAGAAVAVGGGRAMDGAGNYVE
                     PTLFTQATNAMRIAQEEIFGPVLTVMPFDSEEEAVAIANDVPYGLTGYIWTNDVGRAH
                     RVARDLEAGMVWVNSENVRHLPTPFGGVKQSGIGRDGGDYSFDFYMETKNIAIAYGGH
                     KIPRVGVA"
     gene            complement(4720..5124)
                     /locus_tag="VKU03_05825"
     CDS             complement(4720..5124)
                     /locus_tag="VKU03_05825"
                     /inference="COORDINATES: protein motif:HMM:NF014958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-carboxymethyl-2-hydroxymuconate isomerase"
                     /protein_id="HLJ70816.1"
                     /translation="MPHIVVEYSANLAAEIAPARLVEIVHRAVLADPAFEIGAVRTRA
                     VGYEDVMIADGDPANAFIAVTIRIGPGRDAATRKRVSETVAAALMGALASAFERRGLA
                     LSVEVAELDDSAMTRKNNLHARMRAKAGGAPR"
     gene            complement(5268..5936)
                     /locus_tag="VKU03_05830"
     CDS             complement(5268..5936)
                     /locus_tag="VKU03_05830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012555511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLJ70817.1"
                     /translation="MVANSRAAPKGLLLDFGGVISVTFFERHSFTEMLLGLAPGALTW
                     RGPFDPEGDPLWLAMLAGEISERDYWARRASEVGRLVGEDWTMATLIARTRGPDPNLH
                     IRPEAIEAVRRAKARGVKVGVLSNEMELFYGAETVAALAVLREMDAIVDATHTQILKP
                     SARAYALGCEALGVAAGNVVFVDDQPRNVEGARRAGLDAVFFDVTRPAASFAEAARRL
                     GLTP"
     gene            complement(5940..7316)
                     /locus_tag="VKU03_05835"
     CDS             complement(5940..7316)
                     /locus_tag="VKU03_05835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018326291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLJ70818.1"
                     /translation="MPKAYSSNFLRENNAKQVWHPMAHPSEMESAPPRIIVSGEGVTV
                     TDIDGRRAIDAVGGLWNVNLGYSNEAIKKAIADQLAELPYYPAFRGATHPRLIELAHR
                     LIEILAPENMRRAFFTAGGSDSVETALRIARQYWKVRGQPERTKFLALKNGYHGTHFG
                     GASVNGNLNFRRAYEPLLPGVFHTPSPFLYRNPFGETDPDRLVELCLAAAEEEIKFQG
                     ADTIAAFIAEPVLGAGGVIVPPAGFFSKLRALLDRHDILLIADEVVTAFGRTGQWFGC
                     RLEAVKPDMMCLAKAITSGYFPLGACVVNERIESAFQSNRDALGTIYHGYTYSGHPVG
                     CAAALACLDQTFSMDLPGNAAKEGAFLLDEFLKLAEKYEMIGDVRGKGLMLALEVVAD
                     RGQKTRASKAYMSALADHAFEAGAMVRTSANLVILSPPLILKRAGSRQIVEGLDRAFE
                     RAGANAAI"
     gene            complement(7358..8062)
                     /locus_tag="VKU03_05840"
     CDS             complement(7358..8062)
                     /locus_tag="VKU03_05840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008509312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70819.1"
                     /translation="MTALVVSDLVVNRAGLPVVRGASLAAEPARISVLLGANGAGKTT
                     LLEGVSGVAPSAGGLIEIGGAPMHGTRPSVRVRAGLAHVEQGRTVFRQLTTDENLRAA
                     APSKASVAQAYELFPELAKRAQVRAGLLSGGEQQMLVIARALVHRPKVVLIDEMSAGL
                     APVIVKRLMAAVRKLADQGLAVLLVEQFAALALSIGDRAYVLRRGRIVYDGDCASLRQ
                     SPDELHRLYLGEAGQT"
     gene            complement(8059..9771)
                     /locus_tag="VKU03_05845"
     CDS             complement(8059..9771)
                     /locus_tag="VKU03_05845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002967143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ70820.1"
                     /translation="MRASLRAAARILLLSALVGAFVAFVLPGYWVFLATSVVIAAVSL
                     MSLGVVAGRTGMISLCQMTFAGVGAWTVGYLNVAEAPGGLALWIVVGGLAAVPFGVAI
                     GLPALRLRGINLAIVTLGFAAAFDAVLATITFPGQTAFISVVRPELFDSDSGYFLFAL
                     IFFAAIAIALDFIARSRLGAAWLAIRHSERAAAAHGVRIPAAKLSAFAISAFVAGVSG
                     GLLAGYLSTLVSQNFDMMQSLVLFAVATMAGAQTAEGALIGGLLGVVFPEILRRLNLT
                     QDVGNVLFSVGATQALSSGETMSDSLNRALRRWRRAAPARQTRADTASLPPPKHRAAS
                     LDALAVRAVTVRYGAVLALDKVDLTVPAGGVVGLIGPNGAGKSTLIDAVGGFIGYEGR
                     VELAGRPLEGVPAFRRARLGVRRTWQTTRIAPELTVGAYLRLSAGRLDDGEMDAILAW
                     IGCPGPETKVANVDAGARRLLDVAGVAAARPAVVLLDEPAAGQSYDEAIRLSRRIAEI
                     PSLFGSAVLLIEHDMDLVRAACQSVTVLDFGRVIASGPTLDVLTRAEVRKAYLGVDEE
                     TAAA"
     gene            complement(9761..>10252)
                     /locus_tag="VKU03_05850"
     CDS             complement(9761..>10252)
                     /locus_tag="VKU03_05850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004685900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70821.1"
                     /translation="GAAFRLAGVEVTNAAIATLALALLFTVSADLFLMRTRAGLHLRA
                     LSERPMAAELIGVPVRSLSLFVWGVTGAATTLALMIVAPQRAPDFMSLSLLVVPALAA
                     ALIGFFNSFWAALIGGAALGVAESLTSAFPALSEYRAGAPFLVILAVLLWSQRGARWD
                     DAR"
BASE COUNT         1865 a         3395 c         3394 g         1598 t
ORIGIN      
        1 cccgatgctc gcccagcatg gcgtaaccga gcaacaatgg cgcgtcatcc gcgtcttgag
       61 cgaggaaggg ccgctcggcg ccacggaggt tgcggaccgc gccgccattc tcggaccgag
      121 cttgacgcgg atcatcaaga cgttggagcg gcggcgcatg atcaccagtc gccgccttcc
      181 ccatgacggg cgccgcgtcg tactcgaaat cgccgccgcg gggcgagccg tgatcgcgtc
      241 cgccgcgccg gagagacgga agatataccg cgatctcgag cgccgccatg gccaggaggc
      301 gcttgacgac cttctggatc ggctcgaagc gctcatcctg cgcgagcaaa aaggcgcagg
      361 cgacgggaag cgccgaaaag agagggcgca aaagggttga gacggcgagg cggcgcgacg
      421 cctcgtccgc gcgccgagcc gtcaggcgcc ggtttcgtcg cgtacgccgt ttgcaaggct
      481 tccgagcccc gtcgcctcga cctcaacctc gtcgccgggc cgcagccaga caggcgggtc
      541 gcgccgcgcg ccggcgccgc tcggcgttcc ggtgagaata atgtcgcccg gcgcaagcgg
      601 gcagaaggtc gagatatatt cgacgatccg gccgaacggg aacatcatcc gctcggtcga
      661 atcgctttgc cgaacctcgc cgttgacgcg cgtcaccagc gtgaaaggcc cagggcccgc
      721 ctcgtcgagc gtggtgatcc aggggcccag cgcgccgctc gccgcccagt tcttgcccgg
      781 cgtcacattg aacttgccgt gccgcaccca atcgcgaatc gtcccttcat tggcgagcag
      841 gtagccgaac acgtgaccgg tccaatcggc ggcgcgaatc cggcgcccgg ctttgccgat
      901 gacgagaacg atctcgcctt catagtccag ctcccggctt tccggcggac ggaccaaggc
      961 ttcgccgggg ccgacgaagg acgcgggaaa gcggacgaac aggctcggat atttcggctg
     1021 ctcggaattg tccttatatt cggcgttgcg ctccggataa ttgacgccga cgcaaaagca
     1081 tttgccccaa tcgagaagcg gcttctgcag cgcgatctcg ccaagcgcgt aatcggccgg
     1141 atcgcctgcg catgcttcga gccgttgacg cgcgccttcg ccgccctcgc gcgtcagcaa
     1201 atcgtcgagg ccgcgataag catccttgag gcgccggccg acatcaacga cgcctcgttc
     1261 gttgagaaga ccgaaagaat ttcgattcgc agcggtgaaa ctgacgatcc gcatcgccct
     1321 gccctacctg gttcgctgtc tcatccgaaa tggcacgaaa ccgcgccgaa cgcgccgaaa
     1381 tcggcatgaa tccgatcgcc cctgccgacc tcgatcgggc ggatgaacga gccggcgagc
     1441 acgatctcgc cggcttccag ccgctcgccg ttttccgcca ggcgattggc gagccaagcg
     1501 attcccgtcg ccggattgtt gagcacgccg gcggcgagcc cggtctcctc gacctcgccg
     1561 ttcttggcgc agatcgcgcc gatccagcgc aggtcgtgat cgaacggacg aaaccgccgg
     1621 ccgccgagaa tgagcccggc attggcggcg ttgtcggcga tcgtgtcgcg gacggtgcgc
     1681 gtcttgccgg tctcaggatc gacccggaag atgcgcgtat cgaggagctc cagcgcgggc
     1741 gagacgaaat cgctcgccgc caccgcgtcg gaggccgtgc agttcggccc ggcgagcggc
     1801 ttgccgagaa cgaaggctag ctccgcttcg acccgcagcc cgatgaagcg cccgctcgga
     1861 atctcgccgc cgtcggcgaa caccatgtcg tcgagcagga agccggaatc cggctccttg
     1921 acccccaccg ccgcctgcat cgctttcgag gtcaggccga tcttatggcc gacgattcgg
     1981 ccgccgcgct tcagcttgat gtcgagccat gctttctgga ttgcgtagga atccgccatc
     2041 gtcatcgccg gatggtcgag ggtcagcatg cggatctgct tgcgctcgcg ttcggcggcg
     2101 tcgagccgct tggccgccgc aatatggtcg tcgggcccca tccgcgcgcc tttcaaggag
     2161 cgacgattgg cgtcgcgttc agtttcggcc gcaccggttc gacgccggcg aagaccgacc
     2221 cctcctcgaa ccacgagcgc ggcgccggcg ccccccacaa tgtctgacgt tgcgggtcgg
     2281 agaggcccca ccgaatgggt tcgagatcgg gatcgacagt ttggtagtcg gagcaataga
     2341 tctcgacgcg gtggccgtcg ggatcgcgga catagaggaa gaacgcattc gatatgccgt
     2401 gccggcccgg cccacgctcg atattgccga gatagccggt ggtcgccatc aggtcgagca
     2461 ggtcgataat gttgagcggg gtcgggaccc agaacgcgac atggtgaagg cgcggaccca
     2521 gtccgttcgt aaaggcgacg tcatggacgc cgcccttgcg atgcatccaa gccgcccaaa
     2581 gctccgcccc gtcgtcggtg tcggtatatt ccgtcatgcg aaagccgatc gaggcgtaaa
     2641 attccgccga cgcccgcacg tccggcgaaa agcaattgaa atggtcgatg cgcagcggct
     2701 tgacgccgcg atagagcgca tatttctgat ggatcggcgg caacctctcc atgctgacat
     2761 agaattcgag cggtatgccg acattgtcgc gcgtgcgaaa agtgcggcct tgatacggcg
     2821 tctcgaccca gcgcgttgga aatccccgct gatcgaaaaa gctcttggct cgatcgagat
     2881 gatcctcgtc gaagaccttg aaggcgagcg gaccggccag gccgacatcc cctttttcga
     2941 ggatcaggct gtgatggcag cgctcctcca tggcgcgaag gtagattcga ctctcatcct
     3001 cgctcgtcgc ctgcaagccg agcgtatcga cataaaaagc ccttgatgcg gcgagatcgc
     3061 tcaccgtcag cgatgcatgg ctgagcctga caatgttgaa cggcggataa agcacatggg
     3121 ccgggatcgc catgacgatt gctccttacg cgacgccgac gcgcgggatt ttatggccgc
     3181 cataggcgat cgcgatgttt ttcgtctcca tgtagaaatc gaacgaatag tcgccgccgt
     3241 cgcggccgat gccggattgc ttgacgccgc cgaacggcgt cggcaggtgg cggacattct
     3301 cggaattcac ccagaccatg ccggcttcga gatcgcgcgc cacgcgatgc gcgcggccga
     3361 catcgttcgt ccagatatag ccggtcaatc cgtacggcac gtcgttggcg atggcgaccg
     3421 cctcctcttc gctgtcgaac ggcatcacgg tgagaaccgg cccgaatatc tcctcctgcg
     3481 cgatgcgcat cgcgttcgtc gcctgcgtga acaaagtggg ctcaacgtaa ttgcccgccc
     3541 cgtccatcgc ccggccgccg ccgacggcga cggccgcccc ggcttcgcgc gccagagcgg
     3601 cgtagctcag cactttctcg gcatggcgcg gatggatgag cgggccgatc tcggtcgccg
     3661 gatcgagcgg atgaccgacc ttgatcgcgg cgacgcgctt ggcggcgcgg gcgacgaaac
     3721 tgtcatagat cgagcgctgg accagcgcgc gcgacgagga ggtgcagcgc tcgccgttca
     3781 acgagtagat catgaacaga gcggcgtcga gcgcgcgctc ctgatcggcg tcctcgaaga
     3841 tcatcacggg attcttgccg ccgagctcga aatggacgcg cttcagggtc gctgcgcctt
     3901 ggcgcatgat ctgcgagccg gtgacggtct cgccgacaaa ggcgatcgcc ttgatcgcgt
     3961 cgtgctcggt cagcgccttg ccggcggtct caccgaggcc ctggaccaga ttgacgacgc
     4021 ccgccggtcc gccatgggcg cggatcgtct cgtccatgat ctcggtcaaa aggacggcgc
     4081 tgagcgggct ccattccgcg ggcttgtgca cgaccgtaca acccgccgcg agcgccggcg
     4141 cgattttcca ggtcgacagc atgaacggcg tgttccaggg cgtgatcacg ccgaccgggc
     4201 cgatcggctg gcgcatcgtg tagttgatat gcgccgcgtc cggcaaagcg agtccatcgc
     4261 cggcgccggg ggcgcgatcg gcgtagaagc ggaaattctc cgccccgcgc aaagccgcct
     4321 tggccatgta gcggatcggc tggccggtgt cgctgctctc gatcagggcg atctcgtcgg
     4381 cgcgcgcctc gatcgcgtcg gcgatcgcgt gaagaatggc gcgccttttg gcgccgggaa
     4441 cgttccgcca cgtcttgaac gccgccgcgg ccgcggccgc ggcgcgctcg acctcggccg
     4501 ggccgccgga cgcgacgaga cactggcgcc tgttggtcgc ggggtccagg gtctcgaatg
     4561 tcgcgccgga ttgcgacaaa gccggcgcgc cggcgataaa gtgcgggacg acggcgcccg
     4621 cgaagcggcg caagcaagcc tcggcccgct cctgattgtc ggccagcgtg ctcgtcagaa
     4681 tcgaagcttg gttcatcgag cgcactccct ccttctctct caccgaggcg cgccgccggc
     4741 cttggcgcgc attctcgcat gcaaattgtt cttccgcgtc atcgcggaat cgtcgagctc
     4801 ggcgacttcg accgataggg ccaagccccg gcgctcaaaa gccgaagcaa gcgcgcccat
     4861 cagcgccgcc gcgaccgtct cggagacgcg ctttcgcgtc gcggcgtcgc ggcccggccc
     4921 gatccggatc gtcacggcga tgaaggcgtt ggcggggtcg ccgtcggcaa tcatgacatc
     4981 ctcgtagccg accgctcgcg tccggaccgc gccgatttcg aaagccgggt cggcgagaac
     5041 cgcgcgatga acgatctcca ccaggcgcgc cggcgcgatc tcggcggcga gattggcgct
     5101 atattcgacg acgatatgag gcatcggcct tccctttcgc cttgcgggcc aagtcggccc
     5161 gcgaaagcgg cgcgagcaaa gcttggcggc tgatcgcgtt cttttcctgc cagtcgctta
     5221 acatgttaag gacgcccaag ggcttgtcaa atgacgaagc cgccggctca aggcgtaagc
     5281 cccaagcgcc gcgccgcctc agcgaacgaa gccgccggcc gcgtcacgtc gaaaaaaacg
     5341 gcgtcgaggc cggcgcggcg cgcgccctcg acattgcgcg gctgatcgtc gacgaagacg
     5401 acgttcccgg ccgcgacgcc gagagcctcg cagcccagcg cataggcgcg cgccgacggc
     5461 ttgagaatct gggtatgcgt cgcatcgacg atcgcgtcca tctcgcgcag gaccgccagc
     5521 gcggcgacgg tctcggcgcc gtagaagagc tccatctcgt tcgacagaac gccgaccttg
     5581 acgccgcgcg ccttcgcgcg gcgcaccgcc tcgatcgctt cggggcggat atggagattg
     5641 gggtctgggc cgcgcgtgcg cgcgatcagc gtcgccatcg tccaatcctc gccgacaagc
     5701 cggccgactt cgctggcccg ccgcgcccaa taatcgcgct cgctgatctc gccggcgagc
     5761 atggccagcc agagcgggtc cccttccggg tcgaacgggc ctcgccaggt caaggcgccg
     5821 ggcgcaaggc cgagcagcat ttcggtaaag ctgtggcgct cgaagaacgt gaccgaaatc
     5881 acgccgccga aatcgagcag caagcctttc ggcgcggcgc ggctgttcgc gaccatggat
     5941 cagatcgccg cgttcgcgcc ggcccgttcg aaggcccgat caagaccttc gacgatctgg
     6001 cgcgatccgg cgcgcttgag aatgagcggc ggcgacagga tcaccagatt ggccgatgtc
     6061 cgcaccatcg cgccggcttc gaaggcatga tcggcgagtg cgctcatata ggctttgctc
     6121 gcgcgcgttt tttgtccccg atcggcgacg acttccaaag caagcatcaa gcccttgccg
     6181 cgaacatcgc cgatcatttc atatttctcg gcgagcttaa ggaactcatc gagaaggaag
     6241 gcgccctcct tcgccgcatt gccgggcagg tccatcgaga acgtctgatc gaggcaggca
     6301 agcgcggccg cgcagccgac cggatggccg gaataggtat agccgtggta aatcgtccca
     6361 agcgcgtccc tgttgctttg aaacgcgctc tcgatccgct cgttgacgac gcaggcgccg
     6421 agcggaaagt agcctgacgt gatcgccttg gcgaggcaca tcatatcggg ctttacggcc
     6481 tccagccggc agccgaacca ttggccggtg cggccgaaag cggtgacgac ctcgtcggcg
     6541 atcaacagaa tgtcatgccg atcgagcagc gcccgtagct tgctgaagaa cccggccggc
     6601 ggaacgatga cgccgccggc gcccagcacc ggctcggcga tgaaggcggc gatagtatcg
     6661 gcgccctgaa acttgatttc ctcctctgcg gcggccaagc agagttcgac gagacgatcc
     6721 ggatcggttt cgccgaatgg attgcgatag aggaacggcg acggcgtgtg aaagacgccc
     6781 ggcaaaagcg gctcgtaggc gcgacggaaa ttcaggttgc cgttcaccga agcgccgcca
     6841 aagtgagttc cgtgatagcc gttcttcagc gcgagaaatt tcgtccgctc cggctggccg
     6901 cgcactttcc aatattggcg cgcgatgcgt agcgccgttt cgacggaatc cgatccgccc
     6961 gcggtgaaga aggcgcggcg catgttttcc ggcgccagga tttcgatcag ccgatgggcg
     7021 agctcgatca gtctcggatg agtcgcgccg cgaaaagccg gatagtaagg gagttcagca
     7081 agctgatcgg cgatcgcctt cttgatcgcc tcgttcgaat agccgagatt gacgttccac
     7141 agcccgccca cggcgtcgat cgcgcgccgc ccatcgatat ccgtgacggt gacgccttcc
     7201 cccgaaacga tgatgcgcgg cggcgcgctc tccatctcgc tcggatgcgc catggggtgc
     7261 cagacttgct tggcgttgtt ttcgcgcagg aagtttgaag aataggcttt cggcatgatt
     7321 gactctggtt ggctgatcgg cggcattgga tgttcggcta ggtttggccg gcctcgccga
     7381 gatagaggcg gtgcaactcg tccggcgatt ggcgaaggct ggcgcaatcg ccgtcataga
     7441 caatgcggcc gcgccggagt acgtaagcgc ggtcgccgat ggagagagcg agcgcggcga
     7501 attgctcgac gagcaagacg gccagtcctt ggtcggcgag ctttcgcacc gccgccatca
     7561 agcgcttgac gataaccggc gcgagaccgg ctgacatttc atcgatcagg acgactttcg
     7621 gccggtgaac gagcgctcgg gcgatcacca gcatttgctg ctcgccgccc gacaggaggc
     7681 ccgcgcgcac ttgcgcgcgc ttcgccaatt ccggaaacag ctcataggcc tgcgcgacgg
     7741 acgctttgga aggcgctgcg gcgcggaggt tttcatccgt cgtcaattgc cggaagacgg
     7801 tcctgccctg ctccacatgg gcgaggccgg cgcgcacgcg aacgctcggc ctcgtcccgt
     7861 gcatcggcgc gccgccaatc tcgataagac cgccggcgct cggcgcgacg ccggaaacgc
     7921 cttcgagcag cgtcgtcttg ccggcgccgt tggcgccgag cagcacgctg attctggccg
     7981 gctcggcggc gaggctggcg ccgcgcacca ccggcaatcc ggcgcgattg acgacaagat
     8041 cgctgacgac gagcgcggtc atgccgccgc agtctcctca tcgacgccga gataggcttt
     8101 gcggacctcg gcgcgggtga gcacatcgag cgtcgggccg gaggcgatga cgcgcccgaa
     8161 atcgagcacg gtcacggact ggcaagcggc gcggacaagg tccatgtcgt gctcgatgag
     8221 cagcacggcc gagccgaaca ggctcggaat ttcggcgatg cgccggctga gccggatcgc
     8281 ttcgtcatag gattggcccg ccgccggctc gtcgagcaga accaccgccg gacgcgccgc
     8341 ggcgacgccg gcgacatcga gaaggcgccg cgcgccggcg tcgacattcg cgactttcgt
     8401 ctccggcccg ggacagccga tccaagcgag aatggcgtcc atctccccgt cgtcgagccg
     8461 cccagccgac aatcgcagat aggcgccgac ggtcagttcg ggcgcaatgc gcgtcgtctg
     8521 ccaggtgcgg cgcacgccga gccgcgcgcg gcgaaaggcc gggacgcctt cgagcggccg
     8581 tccagcaagt tcgacccgtc cctcatagcc gatgaatccg ccgacggcgt cgatcagggt
     8641 cgatttgccg gcgccattgg ggccgatcag gccgaccacg ccgccggccg gcaccgtcag
     8701 atcaaccttg tcgagggcga gaaccgcgcc atagcgcacg gtaacagccc tcaccgcgag
     8761 ggcgtcgagc gaagcggcgc gatgcttcgg cggaggcagg ctggccgtgt cggcgcgcgt
     8821 ctggcgcgcc ggcgctgcgc gccgccagcg ccgcaacgcg cgattgaggc tgtcgctcat
     8881 cgtctcgccg ctgctcagcg cctgcgttgc gccgaccgaa aagagtacgt tgccgacatc
     8941 ctgcgtcaga ttgagccggc gcaggatttc cggaaagacg acgccgagca agccgccgat
     9001 cagcgcgccc tcggcggtct gcgcgccggc catcgtcgcc acggcgaaga ggaccagcga
     9061 ctgcatcatg tcgaaattct gcgacaccag cgttgaaagg tagccggcaa gcaagccgcc
     9121 ggaaacgccg gcgacaaacg cgctgatcgc aaaagcgctg agcttggccg cgggaattcg
     9181 aacgccatgc gcggcggccg cccgctccga atgacggatc gccagccacg cggccccgag
     9241 ccgcgagcgc gcgatgaaat cgagcgcgat ggcgatcgca gcgaagaaga tcagcgcgaa
     9301 gaggaaataa ccggaatcgc tgtcgaagag ttcgggacga accacgctga tgaacgcggt
     9361 ctggccggga aaggtgatgg tcgcgagaac cgcgtcgaac gcggcggcga agccgagcgt
     9421 cacgatcgcg aggttgatgc cgcgcaaccg cagcgccggc aggccgatgg cgacgccgaa
     9481 cggcaccgcc gccaagccgc cgacgacgat ccataaggcg aggccgcccg gcgcttcggc
     9541 gacattgaga tagccgaccg tccaggcgcc gacgccggca aaggtcatct ggcagagcga
     9601 gatcatcccg gtgcgcccgg cgacgacgcc gaggctcatc agggacaccg ccgcgatcac
     9661 caccgaggtc gcaaggaaaa cccagtagcc cggcaggacg aacgcgacga acgcgccgac
     9721 aagcgccgaa agcaggagga tgcgcgcggc ggcgcgaagg ctagcgcgca tcgtcccacc
     9781 gggcgccgcg ttgcgaccaa agcaggacgg cgaggataac gagaaaaggc gcgccggcgc
     9841 gatattcgct gagcgccgga aaggcgctgg tcaggctctc ggcgacgccc agcgcggcgc
     9901 cgccgatcag cgccgcccag aagctgttga agaatccgat cagcgccgcg gcaagcgccg
     9961 gaacgacgag caggctcagc gacatgaagt ccggcgcgcg ctgcggcgcg acgatcatca
    10021 gcgccaaggt cgtcgcggcg ccggtcacgc cccaaacgaa aagcgacagg ctgcgaaccg
    10081 ggacgccaat cagttcggcc gccatcggcc gctccgaaag cgcgcgcaaa tgcaagccgg
    10141 cgcgcgtgcg catcaaaaag agatcggccg agacggtgaa gaggagcgcc aaggcgagag
    10201 tcgcaatcgc cgcgttcgtc acctcgacgc cggcaaggcg gaaagccgcg cc
//
LOCUS       DATGJW010000192         8355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1486057, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000192 DATGJW010000000
VERSION     DATGJW010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8355
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486057"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VKU03_05855"
     CDS             <1..890
                     /locus_tag="VKU03_05855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ70822.1"
                     /translation="VQKALDGEPYALLGPGYSGSVKVCEPLAAEAGIAEIMGGEAADL
                     TRMGNKYVFRTSFGQQSSMPKIAKYIAENLKAKTAAVVYVNNDFGRGGHDMILKEFAA
                     RGVKVVLDDSTEAGQADFAADAVKVKNANPDDAFIYLNEEESARMLKELRKADVKATL
                     VGETTLIGQKVIELAGDAANGAIGHVGLTVDAPVPALQAYGKRFFAKYHYVPDHNGIK
                     GYLGVYMIKAATEKMGKLDSKGLAAALHGLTITTAMEPNILMDVTIDENGDLDRESFL
                     GKVVNGKQVITEVLPKLKN"
     gene            1111..1986
                     /locus_tag="VKU03_05860"
     CDS             1111..1986
                     /locus_tag="VKU03_05860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70823.1"
                     /translation="MANFIALVIAGLATGALYALVAIGFTLLWQTSQTINFAQGEFVM
                     LPAFFMLAAMNLHLPFWAAILIGIALSTAILGFAFKKLLVDPMLQHGVLPLAIATMAL
                     STFLKESVKTFYSAEASPFPSLAPDMDLHILGVAVSWQNIAVLVVALAAMLALQLLMN
                     RTLLGRQMQAAAQNPLAARILGVPVERMILYTFLINGALVALASLLITPIYLAKFTNG
                     ETLGLAAFVAAIVGGFNQIRGAIVGGLLLGVVDNLAAAYVSTQYRGAIPMLLLIVIIL
                     FRPNGLLGRAEERTV"
     gene            1983..2987
                     /locus_tag="VKU03_05865"
     CDS             1983..2987
                     /locus_tag="VKU03_05865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70824.1"
                     /translation="MNAKARNLLVPSALAIVALFLIFAPLTFHRYGLYISSQWAVMTI
                     AAIGLNLTLGYAGQVSLAQGAFVGIGAYSAAILTTHGWPLIAAIALALVLCFAIGWLL
                     GYPALRVQHHYLAFVTLAFSTLAFLVFRNESWLTGGTEGIDSVPRPSLFGFSLAPHLR
                     YYYFCLFVLALVTLATLGLVRSPWGRAFIALRENPIRAMSLGVDTRRYILMAFAIGSA
                     LGGLSGALYAPLVGLVEPPAFALTLSFDLLMMVIVGGAGYFIGPFVGAMIAVLLPDWL
                     RFLDRYYLILYALLVMALLIYSPTGLVGVADRFFIRRRTKAASTARAALSASMESEGA
                     "
     gene            2984..3727
                     /locus_tag="VKU03_05870"
     CDS             2984..3727
                     /locus_tag="VKU03_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70825.1"
                     /translation="MKTVLEVANLKKSFGGIAAVDGVSFDVREGEILGIIGPNGCGKS
                     TLFNCILGQLAPSEGEVRLDGHVTTGMRPCDLNRLGVSRTFQLLQIFPKLSVRDNLIL
                     AAQEHQGTMLGRLFGRRDAGLAEAAKRMIAFFKLDHLADEIAGGLSYGQQKLLDAAMA
                     FMSGPRLVLLDEPAGGVNLTMLANLKDRLKAFNEERGATFVVIEHNMDFVMSLCSRVL
                     VLAEGKVLAEGLPTDVRANPAVIEAYLGH"
     gene            3771..4454
                     /locus_tag="VKU03_05875"
     CDS             3771..4454
                     /locus_tag="VKU03_05875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018456029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70826.1"
                     /translation="MTILNGTSFKVARSAITTIIGPNGAGKSTVFKTIFGLIRPRSGA
                     IRIGGRDAMKWSQRELIAAGVCYIPQGRNIFPELSVRHNIELGAVAAGPSIKDLPARL
                     DAALDRFPALRRKADRQASTLSGGEQKQLEIVRGLLLDPKLILIDEPSIGLSPLMVQE
                     TFALLTALRRKGVTILMIEQNARSALHVSDYGLVLELGQTRMHDKAEAILNDPRVGRL
                     FLGGATAAA"
     gene            complement(4501..5484)
                     /locus_tag="VKU03_05880"
     CDS             complement(4501..5484)
                     /locus_tag="VKU03_05880"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HLJ70827.1"
                     /translation="MTDRARRRTRSLPADALGIAEAAACLKAGGLVAMPTETVYGLAA
                     DATSDKAVAAIYAAKQRPTFNPLIAHILDRAAAAREAVFDAHAERLTEAFWPGPLTIV
                     APAAASCRVSALARAGLDSVALRAPSHPAARALIEAAGIPLAAPSANRSGHVSPTLAE
                     HVLADLDGRIEIVLDAGPTEAGLESTIVACLGGRPRLLRHGALARETLEETLGFALTA
                     EAPTDEAAPRGPGMLASHYAPAARLRLDARDAATDEAALDFAGALAASRAATRLDLSP
                     SGDLTEAAANLFAYLRALDRTGANKIAVAPIPPHGLGAAINDRLRRAAAPR"
     gene            5594..7384
                     /locus_tag="VKU03_05885"
     CDS             5594..7384
                     /locus_tag="VKU03_05885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HLJ70828.1"
                     /translation="MTYRAPVSDILFSLNHVAGFGEALRQGLFGELDAPTAASVIEEA
                     GRFATDAIAPLNRAGDLHGSKYENGSVATPPGFRDAYRAWAAGGWAGVTGAPEFGGMG
                     LPHAVSAACSELWAGASMGFALCPLLSEAAVNAIARFAAPDLRALYLPKLISGEWTGT
                     MNLTEPQAGSDLNAVKSRAEPAGDGTYRLFGQKIFITYGEHDMADNIVHLVLARLPGA
                     PSGTRGLSLFLAPKFLPRPDGAPGERNDLRCASIEHKLGIHASPTCVMIYGDSGGATA
                     WLVGEENRGLAAMFTMMNNARLGVGLQGVAIADRALQQALAYARERRQGRSGKGGDGA
                     MSPIVEHPDVQRMLLTMRALVHAARGICHLTAVAIDLSRRAKTEKERREAEERAALLT
                     PVAKAFSTDIGDEAASLGIQVHGGMGFIEETGAAQHFRDSRIAAIYEGTNGIQAIDLA
                     LRKLPLAGGEAIRREIADMRAIQSEVAERGGAGFGAAAARLGEALDAFERATRFMSAA
                     RPEPNDALAGATPYLRLFGLVRGGACLAKAGLAAEGLAAAGDRSELGRVALARFFAEK
                     ILTAAPGLAASVESGAGALSDFERAIGGGQ"
     gene            7381..8181
                     /locus_tag="VKU03_05890"
     CDS             7381..8181
                     /locus_tag="VKU03_05890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLJ70829.1"
                     /translation="MSEHVEARREGAALCLILARPEKKNALSGAMYDALTDALNAANK
                     DDTIGAVLIGGKGGVFCAGNDIGDFLKARPGESSSPGWRFIQTLPLLEKPLVAAVEGP
                     AIGVGTTLCFHCDLVYAAPSARFQMPFVDLALVPEAASSLLVPQRFGMAKASEALLLA
                     EPLDAAEAHRLGLVNQVVAAEALFDHALARAQALAAKPRAAMLATRKLLRGDAAAVQA
                     RMREELSAFGEAVRSPEARAIFEAFLRKSLAPRSGERVAAKPPGEGRR"
BASE COUNT         1291 a         2858 c         2763 g         1443 t
ORIGIN      
        1 aggtgcagaa ggcgctcgac ggcgagcctt acgcgctgct cggccccggc tattcgggtt
       61 cggtcaaggt ctgcgagccg ctcgccgctg aggcggggat cgccgaaatc atgggcggcg
      121 aagccgccga tctgacgcgg atgggcaaca aatacgtgtt tcgcacctcc ttcggccagc
      181 aatcgagcat gccgaagatc gccaaataca tcgccgaaaa cttgaaggca aagaccgcgg
      241 ccgtagtcta cgtcaacaac gatttcggcc gcggcggcca cgatatgatc ctgaaggagt
      301 ttgccgcgcg cggcgtcaag gttgtgctcg acgattcgac cgaagcgggc caggccgatt
      361 tcgccgccga cgcggtcaag gtgaagaacg ccaatccgga cgacgccttc atctatctca
      421 acgaggagga gagcgcgcgc atgctcaaag agctgcgcaa ggcggacgtg aaggcgacgc
      481 tggtcggcga gacgacgctg atcgggcaaa aggtgatcga actcgccggc gacgccgcca
      541 acggcgcgat cggccatgtc ggcctgaccg tcgacgcgcc ggtccccgcg ctccaggcct
      601 atggcaagag gtttttcgcc aaataccatt acgtgccgga ccacaacggc atcaaaggct
      661 atctcggcgt ctatatgatc aaggcggcga ccgagaagat gggcaagctc gattcgaaag
      721 gcctcgccgc cgccctgcac ggcctcacca tcaccaccgc gatggagccg aacatcctga
      781 tggacgtgac gatcgacgag aatggcgatc tcgaccgcga aagcttcctc ggcaaggtcg
      841 tcaacggcaa gcaggtgatc accgaagtcc tgccgaaact gaagaattga tcgtcgtctc
      901 gggagagaaa ggcgcgatga gcggagggcc gccctcaccc caaccctctc ccgcaagcgg
      961 gagagggggc gctccgcgcc attcctcggc gccgcgcgtc ccgaccgggc gcgcgggaaa
     1021 aaccccttct cccgcttgcg ggagaaggtg gcgcgaagcg ccggatgagg gtatggcgca
     1081 ggatttcggg gttctgccgg cacaaccgtc atggctaatt tcatcgcgct ggtgatcgcc
     1141 gggctcgcga ccggggcgct ctatgcgctg gtcgcgatcg gcttcaccct gctctggcag
     1201 acgtcgcaga cgatcaattt cgcgcaaggc gagttcgtca tgctgccggc cttcttcatg
     1261 ctggcggcga tgaaccttca tctgccgttc tgggcggcga tcctgatcgg tatcgcccta
     1321 tcgaccgcga ttctcggctt cgccttcaag aagcttctgg tcgatccgat gctccagcat
     1381 ggcgtcctgc cgctcgccat cgccaccatg gcgctgtcga ccttcctgaa ggaatcggtc
     1441 aagaccttct attccgccga ggcgtcgccc ttcccgtcct tggcccccga tatggacctc
     1501 cacattctgg gcgtcgccgt ctcctggcaa aatatcgccg tgctcgtcgt cgcgctcgcc
     1561 gcgatgctcg ccctgcagct cctcatgaac cggacgctgc tcggccggca aatgcaggcg
     1621 gcggcgcaaa acccgctcgc cgcgcgcatt ctcggcgtgc cggttgagcg gatgatcctc
     1681 tatacgttcc tgatcaacgg cgcgctcgtg gcgctcgcct ccttgctgat cacgccgatc
     1741 tatctcgcca aattcaccaa tggcgagacg ctcggcctcg cggctttcgt cgccgcgatc
     1801 gtcggcggct tcaaccagat ccgcggcgcg atcgtcggcg gcctgctgct cggcgtcgtc
     1861 gacaatctcg ccgcggccta tgtctcgacg caatatcgcg gcgcgattcc catgttgctg
     1921 cttatcgtga tcatcctgtt ccggccaaac ggcctgctcg gacgggcgga agagcgcacc
     1981 gtatgaacgc caaggcgcgc aatcttctcg tcccctcggc gctcgcgatc gtcgccctct
     2041 tcctgatttt cgcgccgctg accttccacc gctacggcct ctacatatcg agccaatggg
     2101 cggtgatgac gatcgcggcg atcggcctca atctcaccct cggctatgcc ggccaggtct
     2161 ccctcgccca aggcgccttt gtcgggatcg gcgcctattc ggcggcgatc ctcaccaccc
     2221 atggctggcc gttgatcgcg gcgatcgcgc tggcgctggt cctttgcttc gccatcggct
     2281 ggctgctcgg ctatccggcc ttgcgcgtgc agcatcacta tctcgcattc gtcacgctcg
     2341 ctttctcgac cctcgccttc ctggtctttc gcaatgaatc ctggctgact ggcggcacgg
     2401 aaggaatcga cagcgtgccg cgcccctcgc tgtttggctt ctcgttggcg ccgcatcttc
     2461 gctactacta tttctgcctc ttcgtcctcg ccttggtgac gctggcgacg ctgggcctgg
     2521 tgcgctcgcc ctgggggcgc gcctttatcg cgctgcgcga aaatccgatt cgcgccatgt
     2581 cgctcggcgt cgacacgcgg cgctatatcc tgatggcttt cgcgatcggc tcggcccttg
     2641 gcggcctttc cggcgcgctt tatgcgcctc tggtcggcct ggtcgagccg ccggctttcg
     2701 cgctgactct ctcgttcgac ctcttgatga tggtgattgt cggcggcgcc ggctatttca
     2761 tcggcccgtt tgtcggcgcg atgatcgcgg ttctgctgcc ggactggctg cgctttctcg
     2821 atcgctatta tctcatcctc tacgcgctgc tggtgatggc gctgctgatc tattcgccga
     2881 ccggcctcgt cggcgtcgcc gatcgcttct tcatccgccg ccggaccaaa gccgcctcga
     2941 ccgcgcgggc cgcgctgagc gcctcgatgg aaagcgaagg cgcatgaaaa cggttcttga
     3001 agtcgccaat ctcaagaaaa gcttcggcgg catcgccgcg gtcgacggcg tctccttcga
     3061 cgtgcgcgaa ggcgaaattc tcggcattat cggtccgaat ggctgcggca agtcgacctt
     3121 gttcaattgc attctcggcc aattggcgcc gagcgagggc gaagtcaggc tcgacggcca
     3181 tgtgacgacc ggcatgcggc cctgcgatct caaccggctc ggcgtcagcc gcaccttcca
     3241 gctcctgcaa atcttcccga agctttcggt acgcgacaac ctgatcctcg ccgcgcaaga
     3301 gcatcaaggg acgatgctcg ggcggctttt cggccggcgc gacgccggcc tcgccgaggc
     3361 ggccaagcgg atgatcgcct tcttcaagct cgatcacctc gccgacgaaa tcgccggcgg
     3421 cctttcctat ggtcagcaga aattgctcga cgccgccatg gccttcatgt ccggcccgcg
     3481 cctggttctg ctcgacgagc cggccggcgg cgtcaacctc accatgctcg ctaacctcaa
     3541 ggaccggctc aaggccttca acgaagagcg cggcgcgact ttcgtcgtca tcgagcacaa
     3601 tatggacttc gtcatgtcgc tgtgcagccg cgtgctggtc cttgccgagg gcaaggttct
     3661 cgccgagggc ctgccgacgg atgtgcgcgc caatcctgcc gtgatcgagg cctatcttgg
     3721 acactgaggc aatcctcgaa ttggagaatg tcgtcgccgg ctatgggccg atgaccattc
     3781 tcaacgggac gagcttcaaa gtcgcgcggt cggcgatcac cacaatcatt gggccgaacg
     3841 gcgccggcaa gtcgaccgtg ttcaagacga ttttcggcct gatccggcca cgctccggcg
     3901 cgatccgcat cggggggcgc gacgcgatga aatggtcgca acgcgagctg atcgccgccg
     3961 gcgtttgcta catcccgcaa ggccgcaaca tttttcccga gttgtcggtg cgccacaata
     4021 tcgagctcgg cgccgtcgcg gcggggccat cgatcaagga tctgccggcg cggctcgacg
     4081 cggcgctcga ccgctttccc gccttgcgcc gcaaggccga ccgccaagcc tcgactttgt
     4141 cgggcggcga gcagaagcag ctcgaaatcg tccgcggcct gttgctcgac cccaagctga
     4201 tcctgatcga cgagccgtcg atcggccttt cgccgttgat ggtgcaggag acgttcgcgc
     4261 tcctgaccgc gctgcgccgg aaaggcgtga cgatcctgat gatcgagcag aacgcccgct
     4321 cggcgctgca cgtgtccgat tacggcctgg tgctggagct cggccagacg cgcatgcacg
     4381 acaaggccga ggcgatcctc aacgatccgc gcgtcgggcg cctcttcctc ggcggcgcga
     4441 cagcggcggc gtagcccgaa gggaagggcc ccagggcgcg tcaatcgtcc ttccgccggt
     4501 ctatctcggc gcggccgcgc gacgcaggcg gtcgttgatc gcggcgccga gaccatgggg
     4561 agggatcggc gcgacggcga ttttgttggc gccagtacgg tcaagcgcgc gcaaataggc
     4621 gaaaaggttg gcggcggcct ccgtaagatc gccgctcggc gacagatcga gccgcgtcgc
     4681 cgcgcggctc gccgccaaag cgccggcgaa atcgagtgct gcttcgtccg tcgcggcgtc
     4741 gcgcgcatcg aggcgcagcc gggcggcggg cgcgtaatgc gaggcgagca tgccggggcc
     4801 gcgcggcgcg gcctcgtcgg tcggcgcctc ggcggtaagc gcgaagccga gcgtttcttc
     4861 gagcgtctcc cgcgccagcg cgccatgccg caacaagcgc ggccttccgc cgagacaggc
     4921 gacgatcgtc gattccaaac cggcctcggt cggcccggcg tcgagcacga tctcgatgcg
     4981 gccgtcgaga tcagccagga catgctccgc cagcgtcggg ctgacgtggc cggagcggtt
     5041 ggccgagggc gcggcaagcg ggattccggc ggcttcgatc agcgcccgcg cggcgggatg
     5101 gctcggcgcg cgcaaggcga cgctgtcgag gccggcgcgc gccagcgcgc tgacccggca
     5161 ggaagcggcg gccggcgcga cgatggtcag cggcccaggc cagaacgcct cggtcaggcg
     5221 ctcggcatga gcgtcgaata cggcctcgcg cgccgccgcg gcgcggtcga ggatatgggc
     5281 gatgagcggg ttgaaagtcg gccgctgctt ggccgcgtag atcgccgcga ccgccttgtc
     5341 cgacgtcgcg tcggcggcga ggccgtagac cgtctcggtc ggcatggcga ccaggccgcc
     5401 ggccttcagg caggcggcgg cctcggcgat cccgagcgcg tccgccggaa ggcttcgcgt
     5461 gcgccggcgc gcccggtcgg tcataatgga aagccgatcc gcggcttgcg aggcatatag
     5521 tttacatata aaccgtatcg catatcggca ctatctgggc cttgcctgag ccgtcaagct
     5581 tcatggacga gccatgacct accgcgcccc cgtttcggac atcttattct ccctcaatca
     5641 tgtcgccggc ttcggcgagg cgctccgcca gggcttgttc ggcgagctcg acgcccccac
     5701 cgcggcctcg gtcatcgagg aggccggccg cttcgccacc gacgcgatcg cgccgctcaa
     5761 ccgcgccggc gatcttcacg gctcgaaata cgagaacggc agcgtcgcca cgccgcctgg
     5821 ctttcgcgac gcctaccgcg cctgggccgc gggcggctgg gccggggtga ccggcgcgcc
     5881 ggaattcggc gggatgggct tgccgcacgc cgtcagcgcc gcctgctcgg aactctgggc
     5941 cggcgcgtcg atgggcttcg cgctctgtcc gctgctcagc gaggcggcgg tcaacgccat
     6001 cgccagattc gccgcgccgg atttgcgcgc cctctatctt cccaagctga tcagcggcga
     6061 gtggacgggc acgatgaacc tgaccgagcc gcaagccggc tcggacctca acgccgtcaa
     6121 gagccgcgcc gagccggccg gcgacgggac gtaccgtctc ttcggccaga agattttcat
     6181 cacttacggc gagcacgaca tggccgacaa catcgtccat ctcgtcctcg cgcggcttcc
     6241 cggcgcgccg tccggcacgc gcggcctttc gctgttcctc gcgccgaaat tcctgccgcg
     6301 gcccgatggc gcgccgggcg agcgcaacga tctgcgttgc gcctcgatcg agcacaagct
     6361 cggcatccat gcatcgccga cttgcgtgat gatctatggc gattccggcg gcgcgaccgc
     6421 ctggctggtc ggcgaggaaa atcgcggcct tgccgcgatg ttcacgatga tgaacaacgc
     6481 ccggctgggc gtcggcctgc aaggcgtcgc catcgccgac cgcgcgctgc agcaggccct
     6541 cgcctatgcg cgcgagcgcc ggcagggccg cagcggcaaa ggcggcgacg gcgcgatgag
     6601 cccgatcgtc gagcatcccg acgtgcagcg catgctgctg acgatgcgcg cgctcgtcca
     6661 tgccgcgcgc ggcatctgcc atctcaccgc ggtggcgatc gatctcagcc gccgcgcaaa
     6721 gacggagaag gagcgccgcg aggcggaaga gcgcgcggct ttgctgacgc cggtcgccaa
     6781 ggccttctcg accgatatcg gcgacgaggc cgcctcgctc ggaatccagg tccatggcgg
     6841 catgggcttt atcgaggaga ccggcgccgc gcagcatttc cgcgattccc gcatcgcggc
     6901 gatctacgag gggaccaacg gcatccaggc gatcgacctt gcgctgcgca aattgccgct
     6961 cgccggcggc gaggcgatcc ggcgcgaaat cgccgacatg cgggcgattc aatctgaagt
     7021 cgccgagcgc ggcggcgccg gtttcggcgc ggccgcggcg cggctcggcg aagcgctcga
     7081 cgctttcgaa cgggcgacgc gctttatgag cgcggcgcgg cccgagccga acgacgcctt
     7141 ggccggcgcg acgccgtacc ttcgcctctt cggcctcgtc cggggcggcg cctgcctggc
     7201 caaggccggc ttggcggcgg aaggccttgc ggcggcgggc gaccgctccg aactcggccg
     7261 cgtcgcgctg gcgcgcttct tcgccgagaa aattctgacc gcggcgcccg gcctggcggc
     7321 cagcgtcgaa agcggcgccg gcgcgttgag cgatttcgag cgggcgatcg ggggcggaca
     7381 atgagcgagc atgtcgaggc gcgccgcgaa ggcgcggcgc tttgtctcat cttggcgcgg
     7441 ccggaaaaga agaacgcgct gtccggcgcg atgtacgacg cgctgaccga cgctttaaac
     7501 gcggcgaaca aggacgatac gatcggcgcg gtgctgatcg gcggcaaggg cggtgtgttc
     7561 tgcgccggca acgatattgg cgatttcctc aaggctcggc cgggcgagag ttcttcgccg
     7621 ggctggcgct tcattcaaac cctgccgctt ctggagaagc cgctcgtggc ggcggtggaa
     7681 ggtccggcga tcggcgtcgg tacgacgctg tgcttccact gcgatctcgt ctacgccgcg
     7741 ccgtcggcgc gcttccaaat gccctttgtc gatcttgcgc tcgtgcccga ggcggcgtcc
     7801 tcgctgctcg ttccgcagcg cttcggcatg gccaaggcga gcgaggcctt gctcctcgcc
     7861 gagccgctcg acgccgccga ggcgcatcgg ctcggcctcg tcaaccaggt cgtcgcggcg
     7921 gaggcgctct tcgatcacgc gctcgccagg gcccaggcgc tcgccgccaa gccgcgcgcg
     7981 gcgatgctgg cgacgcgcaa attgctgcgc ggcgacgccg cggcggttca agcgcgcatg
     8041 cgcgaagagc tcagcgcctt cggcgaagcc gtgcgctcgc cggaagcgcg ggcgattttc
     8101 gaagcattcc tgcggaagtc cctcgccccg cgcagcgggg agagggtggc cgcgaagccg
     8161 ccgggcgagg ggcggcgctg agttttgcag gttagcgatt cgaaggacgc gtacctttcg
     8221 ggtcctgcgc ggcccctcat ccggcgcttc gcgccgcctt ctccccgcga agcgggagaa
     8281 gggattccac gatcaccgca gcgccaagct cggcgccggc gcgggcttga gcgcaatcgg
     8341 caggcccgcg gcgac
//
LOCUS       DATGJW010000193         3825 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1174192, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000193 DATGJW010000000
VERSION     DATGJW010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3825)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3825)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3825
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1174192"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..579)
                     /locus_tag="VKU03_05895"
     CDS             complement(169..579)
                     /locus_tag="VKU03_05895"
                     /inference="COORDINATES: protein motif:HMM:NF025120.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ70830.1"
                     /translation="MTKSYRAIPSDDIIASLPRERQERIRARAAALIAEEMALRDLRR
                     ARRITQEEVAEQLGGRQVYVSRFEQRADMKLSTLRDYVRAIGGDLQLMVTFPEGKTVR
                     IKDIGEAGSPSTRTRVGKQKRERKDLTRSRVKSA"
     gene            complement(576..944)
                     /locus_tag="VKU03_05900"
     CDS             complement(576..944)
                     /locus_tag="VKU03_05900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003497284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLJ70831.1"
                     /translation="MTWTVAFHPVFRAEFDSFSESVQDELLAMTELLKAAGPWMKRPG
                     ADTLAGSKHANMKELRFDAAGGVWRVAYAFDPDRQAILLVAGDKSGVSQKRFYKGLIA
                     RADQRFDEHLAALHSARQRK"
     gene            complement(1055..1636)
                     /locus_tag="VKU03_05905"
     CDS             complement(1055..1636)
                     /locus_tag="VKU03_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70832.1"
                     /translation="MRLPEVERGDGLANRLLIGFISLVTRMRLPDAARVAFYHKDFGA
                     PIGAWTQAAMRGASAWSVGERELIAAMVAKWNSCAFCEGAHGAVAAHQMQRPLVDAAL
                     ADFRAAPISERLKATLAFLEIMTLRPAELTRIHAKAVLDAGVSAEALTDAIAVGAIFN
                     IVTRYADALDFALPTPDEFKRAASMLLKRGYAS"
     gene            complement(1623..1961)
                     /locus_tag="VKU03_05910"
     CDS             complement(1623..1961)
                     /locus_tag="VKU03_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70833.1"
                     /translation="MNAPARPDHAALAATLRRRILEEPGETPPAARQAAAQRAAGGPP
                     TQSPYDDLARQIGEAAYRVTDAQVASALSAAGSDKAAFEIIIAASVGAGLFRWGQAMR
                     ALKEASDAPA"
     gene            complement(1970..3142)
                     /locus_tag="VKU03_05915"
     CDS             complement(1970..3142)
                     /locus_tag="VKU03_05915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isovaleryl-CoA dehydrogenase"
                     /protein_id="HLJ70834.1"
                     /translation="MIPNAPRPFNFDLGESAETIRETTQRFAEAEIAPRAAAIDRDNL
                     FPRDLWPKMGALGLHGITAPEEHGGLALGYLEHCVAMEEVSRASGAVGLSYGAHSNLC
                     INQIARNGNDEQKSRYLPKLISGEHVGALAMSEPNAGSDVVSMTTRAEKKGDRYLLNG
                     AKMWITNGPVADTLVVYAKTDPSAGARGITAFLIERGMKGFSHGTKLDKIGMRGSDTS
                     ELTFQDCEVPEQNVLGEVGRGVSVLMSGLDYERVVLAAGPLGLMQSAMDAVLPYVHQR
                     KQFGQPIGSFQLVQGKLADMYVTMNAARAYVYAVAKACDRGETTREDAAGAILYAAER
                     ATQVALDAIQLLGGNGYTNDYPTGRILRDAKLYEIGAGTSEIRRMLIGRELFAKSE"
     gene            3336..>3825
                     /locus_tag="VKU03_05920"
     CDS             3336..>3825
                     /locus_tag="VKU03_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ70835.1"
                     /translation="MLQISRKEQKDATQRRLIEVGLDFFASRGFAGSNTADFAKAAGV
                     SHGAVFLHFPTREVLVLRVLDAFGDRLSAALKAAMQGEATFEGALRAHLGVLREYEPF
                     YARLVQEGPLLPIKARSMLFILNASVSHRLKLVAEAEAAAGKMRKFAPHLLFNTWLAL
                     LHY"
BASE COUNT          642 a         1227 c         1246 g          710 t
ORIGIN      
        1 cggcccgcgc ctccatttcg gcgacagcct cggggtaggg gacgagcccg gacgaggtcg
       61 cccactcgac cggcggcgag ccgggggcgg ggagaaagga tgcttggagg tcggaacgaa
      121 aactgggcaa atagcggctg ccttagggaa actaaataat cgtcggcgct acgcagattt
      181 cacgcggctt ctcgtgaggt ctttcctttc gcgcttttgc ttcccaacgc gcgtgcgcgt
      241 cgaaggcgat cctgcttccc caatatcctt gatgcgcacc gttttgccct caggaaatgt
      301 caccatgagc tgaagatcgc cgccgatcgc acggacataa tcccgcaagg tcgaaagctt
      361 catatccgcg cgttgttcaa agcgagagac atagacttgc cgcccgccga gttgctcagc
      421 gacctcctcc tgggtgatgc gtctggcgcg gcgcaaatca cgcagcgcca tttcttcagc
      481 gatcaacgct gcggcccgcg ccctgatgcg ctcctgacgc tctcgcggaa gcgaggcgat
      541 tatatcgtcg ctcggaattg ctcggtaaga tttagtcatt tcctttgcct cgccgaatgc
      601 agcgcggcca gatgctcgtc gaagcgctgg tccgcccttg cgatcaggcc tttgtagaaa
      661 cgcttctgcg atacgccgga tttatcgccg gcgaccagca ggatcgcttg ccgatccgga
      721 tcgaaagcgt aggcgactcg ccatacgccc cctgccgcgt cgaagcgcaa ctccttcata
      781 ttcgcatgtt tcgaccccgc cagcgtgtcg gcgcccggcc gcttcatcca aggccctgcc
      841 gctttgagaa gctccgtcat cgccagcaat tcgtcctgga cggactccga aaagctatcg
      901 aattcggccc gaaagaccgg atgaaaagcg acggtccagg tcaaatttat accttagaat
      961 atatatgtat tgcaaggggt ataattggcg gccaagccga tgcgcttgga cgcggcggcc
     1021 taatcgcttt cgctaacggc tccaatattg ctgctcacga cgcatagccc cgcttgagca
     1081 gcatgctcgc cgcgcgcttg aactcatccg gcgtcggcaa cgcgaaatcg agggcgtcgg
     1141 cgtagcgcgt gacgatgttg aagatcgcgc cgaccgcgat ggcgtcggtc agcgcttcgg
     1201 cgctcacgcc ggcgtcaagc acggccttcg catggatgcg cgtcagttcg gcgggccgaa
     1261 gcgtcatgat ctccaagaag gccagcgtcg ctttcagcct ctcggaaatc ggcgcggcgc
     1321 gaaagtccgc gagcgcggcg tcaaccaacg gtctttgcat ctgatgggcg gcgaccgcgc
     1381 catgggcgcc ttcgcagaaa gcgcacgaat tccacttcgc gaccatggcg gcgatcaatt
     1441 cgcgctcgcc gacgctccat gcgctcgcgc cgcgcatcgc cgcctgcgtc caggcgccga
     1501 tcggggcgcc gaagtccttg tggtagaacg cgacgcgcgc cgcatccggc agccgcatcc
     1561 gcgtaaccag cgaaatgaac ccgatcagca ggcgattggc caagccgtcg ccgcgctcga
     1621 cttcaggcag gcgcatcgct cgcctccttc agcgcccgca tcgcctgccc ccagcggaag
     1681 agaccggcgc cgacggaagc ggcgatgatg atttcgaacg cggccttgtc gctccccgcc
     1741 gccgaaaggg cgctcgccac ctgcgcgtcg gtgacgcgat aagcggcttc gccgatttgg
     1801 cgcgcgaggt cgtcgtaggg cgattgcgtt gggggcccgc cggcggctct ttgcgccgcc
     1861 gcctggcgcg ccgcgggcgg cgtttcgccg ggctcttcga gaatcctgcg ccggagcgtc
     1921 gcggcgaggg cggcgtgatc agggcgggcg ggcgcgttca ttccggacct cattcgctct
     1981 tcgcaaacaa ttcccgcccg atcagcatgc gccggatctc gctcgtcccg gcgccgatct
     2041 cgtagagctt ggcgtcgcgc agaatgcgcc cggtcggata gtcgttggtg tagccgttgc
     2101 cgccgaggag ctggatcgcg tcgagcgcga cttgggtcgc gcgctcggca gcgtagagga
     2161 tggcgccggc ggcgtcctcg cgcgtcgtct cgccgcggtc gcaggctttg gccaccgcgt
     2221 agacataggc gcgcgcggcg ttcatcgtca cgtacatatc ggcgagcttg ccctggacga
     2281 gctggaacga gccgatcggc tggccgaact gcttgcgctg atggacgtag ggcaggaccg
     2341 cgtccatcgc gctctgcatc aggccgagcg gcccggcggc gagaacgacg cgctcgtaat
     2401 cgaggccgga catcagcacg ctgacgccgc ggccgacctc gccgagcacg ttctgctccg
     2461 ggacttcgca gtcctggaag gtcagctcgg acgtgtcgga gccgcgcatg ccgatcttgt
     2521 cgagcttcgt tccgtgcgag aagcccttca tgccgcgctc gatcagaaag gcggtgatgc
     2581 cgcgcgcgcc ggctgacgga tcggtcttgg cgtagacgac gagcgtatcg gcgacgggcc
     2641 cgttggtgat ccacatcttg gcgccgttca gcaggtagcg atcgcccttc ttctcggcgc
     2701 gcgtcgtcat cgagacgacg tcggagccgg cgttcggctc ggacatggcg agcgcgccga
     2761 catgctcgcc ggaaatcagc ttgggcaggt agcggctttt ctgctcgtca ttgccgttgc
     2821 gcgcgatctg attgatgcag agattggaat gcgcgccata ggacaagccg accgcgcccg
     2881 aggcgcgcga gacttcctcc atcgccacgc aatgctcgag atagccgagg gcgagcccgc
     2941 catgctcctc gggcgccgtg atcccatgca ggccgagcgc gcccattttc ggccagagat
     3001 cgcgtgggaa gagattgtcg cgatcgatgg cggcggcgcg cggcgcgatc tcggcttcgg
     3061 cgaatcgctg cgtcgtctcg cggatcgtct cggcgctttc gccaaggtcg aaattgaagg
     3121 ggcggggggc gttagggatc atggcggctg agagtccttg atgcgcaacg cgatgcggcc
     3181 cgagcgtagc gcattttcgg gagggccgac gtcttggctt cggacatgga ggcctccatg
     3241 cgcaaagaat agagcacaga gccgcgaccg gggagggctt gactcgaagg gcttcatggt
     3301 ttaatgagta gatactcact gagtttgtaa tcaccatgct ccagatctcg cggaaagagc
     3361 agaaagacgc cacccagcgc cgcctcatcg aagtcggcct ggatttcttc gcctcgcgcg
     3421 gcttcgccgg ctccaacacc gccgatttcg ccaaagccgc gggcgtctcg catggcgcgg
     3481 ttttcctgca ctttcccaca cgcgaagtcc tggtcctgcg cgtgctcgac gcgttcggcg
     3541 atcggctgtc ggcggcgctc aaggcggcga tgcagggcga ggcgacgttc gagggcgcgc
     3601 tgcgcgccca tcttggcgtg ttgcgcgaat acgagccgtt ctacgcgcgg ctggttcagg
     3661 aagggccgct gctgccgatc aaggcgcgca gtatgctgtt cattctcaac gcgtcggttt
     3721 cgcatcgctt gaagcttgtt gcggaggccg aagcggcggc cggcaagatg cgcaagttcg
     3781 cgccgcatct tctgttcaac acctggctgg cgctgctcca ctatt
//
LOCUS       DATGJW010000194         3645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_449690, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000194 DATGJW010000000
VERSION     DATGJW010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3645
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_449690"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1254)
                     /gene="gltX"
                     /locus_tag="VKU03_05925"
     CDS             complement(<1..1254)
                     /gene="gltX"
                     /locus_tag="VKU03_05925"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009332 - glutamate-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004818 - glutamate-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLJ70836.1"
                     /translation="MSARPIVRFAPSPTGRIHIGNARTALLNWLFAQREGGRFILRFD
                     DTDRERSREEYARSIEEDLAWLSVKPDRIERQSQRLARYEAAAEALKSMGRLYPAYET
                     PEELERRRKRQQARGLPPIYDRAALKLSTEERIALEAQGRKPHWRFKLAPETVRWDDL
                     ARGESHIDCASLSDPVLAREDGTFLYTLPSVVDDIDMGVTHVIRGEDHVTNTAVQIQI
                     FQALAGAAPLFGHHNLLTDASGEGLSKRTGSLSISSLRDSGVEALAAAALAVLTGSAE
                     AVRPVASLDELARSFDLSHLSHGAARFDEAELRALSARTLHILPYSAVADRLSALGVA
                     GGEAFWLAVRGNLSTLAEAAEWRRVVDGAITPIVEEPAFLAEAAGLLPEEPWDQSTWG
                     QWTGKLKEATGRKGRALYHPLRLALT"
     gene            complement(1383..3062)
                     /locus_tag="VKU03_05930"
     CDS             complement(1383..3062)
                     /locus_tag="VKU03_05930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding dehydrogenase"
                     /protein_id="HLJ70837.1"
                     /translation="MAAIVGGEAATDADVVIVGAGLAGLVAACELAEAKKKVVIVEQE
                     GEQSLGGQSFWSLGGLFLVDSPEQRRMRIRDSFDLALEDWMGAAGFDRAEDYWPRLWA
                     EAYVAFAAGEKRAWLAERGLRFFPVVGWAERGGHNAIGHGNSVPRFHLTWGTGPGVLE
                     PFAQRALAAAAEGLIVFKFRHRVAEITRTGGAVDGVAGEILEPSAAPRGEKSSRAASG
                     GFALKAPAVIVASGGIGGNHDLVRRNWPKSLGEPPKRMITGVPAHVDGSMLAVAEAAG
                     ARLINRDRMWHYVEGVKNWAPIWPDHAIRILPGPSSLWLDARGKRLPVPFYPGFDTLG
                     TLRYIRTTGYDHTWFVLTQKIIAKEFALSGSEQNPDFTAKSWRQVIARARAGTPGPVK
                     AFMEKGEDFIVERELPDLVRRMNALTGEPLLDLETVEREIRARDRQIDNPFAKDMQIV
                     AMRGARAYLGDRLIRVARPHKLLDPKAGPLIAVRLNILTRKTLGGIETDLDSRALGAD
                     GQAVRGLYAVGEAAGFGGGGMHGYRALEGTFLGGCIFSGRAAGRAAAKATG"
     gene            3255..>3645
                     /locus_tag="VKU03_05935"
     CDS             3255..>3645
                     /locus_tag="VKU03_05935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70838.1"
                     /translation="MPARILLGLLGLLNLGNGLLMLAAPLRWYGLVPGVAATGPFNHH
                     FVIDIALAYLASGAAMAAGFREGRRAAAFALAGAIWPALHAVFHIAEWLADGFPADAM
                     DAAAQILGVVLVGFLGAGLAVWRAQKEG"
BASE COUNT          580 a         1266 c         1231 g          568 t
ORIGIN      
        1 ggtcaaggcg aggcgcagcg ggtgataaag ggcgcggccc ttgcgtccgg tcgcctcttt
       61 gagcttgccc gtccactggc cccaagtcga ttgatcccag ggctcctcgg gcagcaggcc
      121 ggccgcctcg gccagaaagg cgggctcttc gacgatcggc gtgatcgcgc cgtcgacgac
      181 gcgccgccac tcggccgctt cggcaagcgt cgaaagattg ccgcgcacgg cgagccagaa
      241 ggcttccccg cctgcgacgc cgagcgcgct gagccgatcg gcgaccgccg aataaggcag
      301 gatgtgaagc gtgcgggcgg aaagcgcgcg cagctccgct tcgtcgaaac gcgccgcgcc
      361 atgcgacaga tggctcaggt cgaagctccg ggcgagttcg tcgagcgagg cgaccgggcg
      421 caccgcctcg gccgagccgg tcagcacggc gagcgcggcg gcggccaaag cctcgacgcc
      481 gctgtcgcgc aaggaggaga tcgagagcga gccggtgcgc ttggaaagac cttccccgct
      541 ggcgtcggtc aggagattgt ggtggccgaa aagcggcgcc gcgccggcca gcgcttggaa
      601 aatctggatc tgcaccgccg tattggtgac atggtcctcg ccgcggatga catgggtgac
      661 gcccatgtcg atgtcgtcga cgaccgaggg cagcgtgtag agaaaggtgc cgtcctcgcg
      721 cgccaggacc gggtcggaca gcgaggcgca atcgatatgg ctctcgccgc gggcgaggtc
      781 gtcccaacgg acggtttcgg gggcgagctt gaagcgccaa tgcggcttgc gaccctgcgc
      841 ctccaaggcg atccgctcct ccgtggagag cttcagcgcg gcgcggtcat agatcggcgg
      901 caggccgcgc gcctgctggc gcttgcgccg gcgctcgagc tcttccggcg tctcgtaagc
      961 gggatagagc cggcccatgc ttttcaacgc ctcggcggcc gcctcgtagc gggcgaggcg
     1021 ctgcgactga cgctcgatcc ggtccggctt cacgctcagc caggcaaggt cctcttcgat
     1081 cgagcgggca tattcctcgc gcgagcgctc gcggtcggta tcgtcgaagc gcaaaatgaa
     1141 ccggccgccc tcgcgctggg cgaacagcca gttgagcaag gccgtgcggg cgttgccgat
     1201 atggatgcgc ccggtcggcg aaggcgcgaa gcggacaatc ggacgtgcgg acatcgcgcc
     1261 ttcatagaag ccccgcgccg cgcctgcaac gtgccccaga cgatgccgtc cttacccgcc
     1321 ggcgcgctgg tcaaggcgcg gcgtcgccgt acctcctccg cgaagcggaa gcgagaaggg
     1381 gctcacccgg tcgctttcgc cgccgcccgc ccggcggcgc ggccggagaa gatgcagccg
     1441 ccgaggaagg ttccttccag cgcgcgatag ccatgcatgc cgccgccgcc gaagccggcg
     1501 gcttcgccga ccgcgtagag gccgcgaacc gcttgcccgt cggcgccgag ggcgcggctg
     1561 tcgaggtcgg tctcgatccc gcccagcgtc ttgcgagtca aaatattaag ccgaaccgca
     1621 atcagcggac ccgctttcgg atcgagcagc ttgtgcggcc gggcgacgcg gatcagccgg
     1681 tcgccgagat aggcgcgggc gccgcgcatc gcgacgatct gcatgtcttt ggcgaaagga
     1741 ttgtcgatct ggcggtcgcg ggcgcggatt tcgcgctcca ccgtctcaag gtcgagcaag
     1801 ggctcgccgg tcagcgcgtt catccgtctg accaggtcgg gcagctcgcg ctcgacgatg
     1861 aaatcctcgc ccttctccat gaaggccttc accggccccg gcgttccggc ccgcgcccgg
     1921 gcgatcacct gccgccaact cttggcggtg aaatccggat tctgctcgga gccggacagc
     1981 gcgaattcct tggcgatgat tttctgcgtc agcacgaacc aggtatgatc gtaccctgtc
     2041 gtgcggatat agcggagcgt gcccagcgtg tcgaagccgg gatagaaggg tacgggcagg
     2101 cgcttgccgc gcgcgtcgag ccacagcgag gaggggccgg gcaggatgcg gatcgcatga
     2161 tccggccaga tcggcgccca gttcttgacg ccctcgacat agtgccacat gcgatcgcga
     2221 ttgatcaggc gagctccggc cgcctcggcg accgccagca tgcttccgtc gacatgcgcc
     2281 ggcacgccgg tgatcattcg ctttggcggc tcgccgaggc ttttcggcca gttccggcgc
     2341 accagatcgt ggttgccgcc gatgccgccc gacgcgacga tcaccgccgg cgccttgagc
     2401 gcgaagccgc cactcgctgc gcgcgagctc ttctcgccgc gcggcgcggc gctcggctcc
     2461 agaatctcgc cggcgacgcc gtcgaccgcg ccgccagtcc gtgtgatttc ggcgacgcgg
     2521 tggcggaact tgaagacgat gagcccctcc gccgcggcgg ccaaagcgcg ctgggcgaag
     2581 ggttcgagca cgccgggccc ggttccccag gtcaggtgaa agcgcggcac ggaattgccg
     2641 tggccgatcg cgttgtggcc gccgcgctcg gcccagccga cgacgggaaa gaagcgcaag
     2701 ccgcgctcgg ccagccaggc ccgcttctcg cccgccgcga aggcgacata ggcctcggcc
     2761 caaagccgcg gccagtaatc ctcggcgcgg tcgaagcccg cggcccccat ccaatcttcc
     2821 agcgccagat cgaacgaatc gcggatgcgc atccgccttt gctcgggcga atcgaccagg
     2881 aacaagccgc cgagcgacca gaaggactgg ccgccgagcg attgttcgcc ttcctgctcg
     2941 acgatgacga cctttttctt cgcctcggcg agttcgcaag ccgcgaccag cccggccaag
     3001 ccggcgccga caatgacgac atccgcgtcc gtggccgcct cccctccgac gattgcggcc
     3061 aatcttccat gagcttcggg ataattccag ccgcaaggcg aagagcggtc acaaaagctc
     3121 cgattggccc gtcttaagcg caggatcgtc attgcgcgcg aagcgaagca atccattcgt
     3181 aaagcgccgc tcgaggaaat ggatcgcttc ggcgcttcgc tccccgcaat gaccggcgaa
     3241 aaacgaggcc gtcgatgccc gcccgcattc ttctcggcct tctcggcctg ctcaatctcg
     3301 gcaatggcct cctcatgctg gccgcgccgc ttcgctggta tgggctcgtc ccgggcgtcg
     3361 ccgcgaccgg cccgttcaac catcatttcg tcatcgacat cgccctcgcc tatctcgcca
     3421 gcggcgccgc tatggccgcc gggttccggg aaggccgccg cgccgccgct ttcgctctgg
     3481 ccggcgcgat ttggccggcg ctgcacgccg tcttccatat cgccgaatgg ctcgccgacg
     3541 gatttccggc cgacgcgatg gacgccgccg cgcaaatcct cggcgtcgta ctcgtcggct
     3601 ttctcggcgc cgggctcgct gtgtggcggg cgcaaaaaga aggag
//
LOCUS       DATGJW010000195        12569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_866306, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000195 DATGJW010000000
VERSION     DATGJW010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12569
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_866306"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(224..1867)
                     /locus_tag="VKU03_05940"
     CDS             complement(224..1867)
                     /locus_tag="VKU03_05940"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLJ70839.1"
                     /translation="MRRFALVAAFALIGVAAAEAADLDAAAPESAPYAGPRWQGFTAG
                     LHAGGLFTSGDGETMTWFPATAPTTGLPGAFTLSPAGFLGGVQMGYSKQWGSFVFGGV
                     ADYDLAASRGSASASGLYLGTPFTVSQSQQLRSLGTVRAKIGFAPIDDALIYATGGFA
                     FGQTSLSTNTSFATGAQYSGSHSDLSIGFAVGAGVEYAIDRNWSVAAEALYYDLGRSE
                     VVGLPNFAISAQSDTSAAFKGYVLRLGLNYEFDGDAGESGYSPQPAQSDITATFGERI
                     GMSIGSAKMNLYDSSGQVLLSRLTYHDPSAVTGEVFGRIDDSSGVFIKGFAGIGRQSG
                     GNLQDEDFPPGISPYSSTNSPQQYGRLGYATFDAGYYAFGTSWYKLGGLAGFNYLEDR
                     FNAFGCTETASNPLICPAGVVSASNLTISDDNIWRSVRLGLAGKANLPGGFTIAAEAA
                     WLPITFFNGKNDHWLRTPTDFFGPLPETGIGHTGFQVEGEVNYALSPNFDIGIGARYW
                     ALSAKGQVQFQDATPSGAAQVATFSTQRFQAFAQSSYHF"
     gene            2362..3003
                     /locus_tag="VKU03_05945"
     CDS             2362..3003
                     /locus_tag="VKU03_05945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase-like cysteine peptidase"
                     /protein_id="HLJ70840.1"
                     /translation="MKTGLLRVLVSVCLMAALFVARTISAEAGSLPRASYAAIGGATA
                     VPYGWVDFCHRERAECLGPVLPALDIKLTNATWAKLDHINRMVNQAITPVTNLEHWGT
                     MLDHWDYPLDGKGDCKVYALYKRKLLIDMGFPRQALLMTIVRDLDGEGHTILTVKTNR
                     GEFVLDNLSDEIRAWDATGYRYIKRQSQEDPNVWVAVGADATAQASAATAANE"
     gene            complement(3038..3964)
                     /locus_tag="VKU03_05950"
     CDS             complement(3038..3964)
                     /locus_tag="VKU03_05950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division plane positioning ATPase MipZ"
                     /protein_id="HLJ70841.1"
                     /translation="MHFDDDQPELRSAHVIVVGNEKGGAGKSTLSIHLSVALLKAGRR
                     VATIDLDTRQRTLTRFFENRRSWAQSASWPIETPFHCALERGESGDVRQNEAKEFALF
                     ADAIGAVEHEYEFVVIDTPASDSYLMRLAHSLADTLVSPLNDSFIDVDVFSRVTHDRA
                     QRGAVAQYADLVLEARRKRRLVDNGVIDWVMTRNRIASLASNNAKQIAISIAKLGAEL
                     QFRAVDGLHDRVIFRELFPIGLTALDPIEAGAESRALTASQQAARREIESLVENLRLP
                     AATPGLAHLESRRLWHAETSRYYRQAAESRPD"
     gene            complement(4774..6171)
                     /locus_tag="VKU03_05955"
     CDS             complement(4774..6171)
                     /locus_tag="VKU03_05955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HLJ70842.1"
                     /translation="MQGSALMNAAGRKQRAALVSILASAALALAKLAAGFLSGSLALT
                     SEGGHSALDVGATILTFFAVREGDKPADEEHPFGHAKFEAVAALAETGLLAVLAFFVA
                     FEAVRRIAGEAQTIVHATPLAFAVVIVAIAVDAARWRGLAKIAADTKSDALAADALHF
                     SSDLVSSFLVLAGLVATEAGVANADAFAAVGVALFIAFAGYRLGRRTIDTLVDTAPQG
                     LAKRLGALIEAVPGVAGVDYVRLRRSGAQTIGEVGVFVSRTLPLERVATIKTDILQKI
                     ALAEPQAALTVTANPRALDNESVLERVLLIAGRRRLPVHHVTIQEIEGRRSVSLDMEI
                     DGEMSLGEAHEIASRLEAAIKREIGPDIEVETHIEPMAIEEIPGREAEPDIVKAIAAS
                     LARRAAETGRLREIHDVRARHTPGGLLVNFHCRIDPNVSVSATHEEVDALERTVRDDF
                     PDIARVIGHAEPTRG"
     gene            6249..6689
                     /locus_tag="VKU03_05960"
     CDS             6249..6689
                     /locus_tag="VKU03_05960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452446.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ70843.1"
                     /translation="MEYLHTMIRVTDLDQSLDFFVNKFGLAEVRRINNEKGRYTLVFL
                     AADEDIDRAKQSQAPLVELTYNWDPEVYTGGRNFGHLAYRVDDIYRFCDKLMKAGITI
                     NRPPRDGHMAFIRTPDNISIELIQKGEALPPTEPWRSMPNTGVW"
     gene            6828..7787
                     /locus_tag="VKU03_05965"
     CDS             6828..7787
                     /locus_tag="VKU03_05965"
                     /inference="COORDINATES: protein motif:HMM:NF023697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3618 domain-containing protein"
                     /protein_id="HLJ70844.1"
                     /translation="MADERSLDELRRDADKARADLVRTVDELRARASPTGLMSAAARS
                     ARDNPLQTVALAAGLAYPLLKTVRSIPAPLLMIGAGLFLSGTSAGRSASRKAVAFGSD
                     MTNKVGALASDMSKTAAERANAGLAAARDAAAAAGATLSDKMSAVKEKASSLAAASAD
                     QLQRAGAATAAAQSDLAARAADAASGFSPQASKDQLSRLVEEAGAAAADTIRRNPLLV
                     GGVGAAVGAVIAGLLPSSKTEDRWVGPVSDRLKSGARETASQGYEAARDAAAAAYDSA
                     AERAGQEGLDADGVKAAAEDFAERARKVANSALDAAAQAEKAH"
     gene            7809..8345
                     /locus_tag="VKU03_05970"
     CDS             7809..8345
                     /locus_tag="VKU03_05970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HLJ70845.1"
                     /translation="MSDPGSFRENDPNSGDLADQAVAAGKQAKNAAADFAQTTMDTVK
                     RRAGDAADAAKDIAGQAGERLQEEAMGKKGEAADYVSRLADAMRRAAGEFESDLPAAA
                     SYIRSAADQVESVSESVRNGDFKDLLEGAQSFARSQPTAFVGLAALAGFGLVRFLKSS
                     AHETPKADDAAQTRYDYH"
     gene            8359..8754
                     /locus_tag="VKU03_05975"
     CDS             8359..8754
                     /locus_tag="VKU03_05975"
                     /inference="COORDINATES: protein motif:HMM:NF018980.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phage holin family protein"
                     /protein_id="HLJ70846.1"
                     /translation="MYPFSTDRSIGELLGEALAELSKLIQTEFAIARTEMSEKVAKML
                     GAAKLIGVGAAIALAALFIILMAISAALMAAGLPGWASYLLTGGVAAIIGVALIMAGA
                     SRLSSRSLKPATTLDELRQDRVTAKAMTQ"
     gene            8751..9536
                     /locus_tag="VKU03_05980"
     CDS             8751..9536
                     /locus_tag="VKU03_05980"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70847.1"
                     /translation="MSAPEKIQQSGGPFLESLRNAAAENPAAAALIGAGVLWLFAGRV
                     QIGPMAKAGAARIADAAAEAQRATSDAMASTWRSAKENVSGLASRAAAQVKRAGDDAG
                     EAAPPDLPEPQRESGAPGAPMQEQRAAISRRVADGLHAGAETFSSARGQLSNVLQSQP
                     LALGLIGLAVGAAVAAALPSTRVENETLGETSDALRDQAKQQMDRAADAASAAATKAT
                     EEARAQNLTADALKAKVEETATKISGVADSAAETLRDKMRPAP"
     gene            9584..10147
                     /locus_tag="VKU03_05985"
     CDS             9584..10147
                     /locus_tag="VKU03_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894428.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin"
                     /protein_id="HLJ70848.1"
                     /translation="MEALTGWGRPTGDASALVQPRSAETFRFGDDGAIPNHPFWPLIV
                     YRAAVRFPGKLDPAAVLEDLFHKNGWGGMWRDGIYDYAHYHSQIHEALGVARGAAEVE
                     FGGAGGRILPLKAGDVAILPAGVGHRRVAASKDFLVVGAYPPAGQYDECAKPEDRQGA
                     LARIPATPRPLMDPVYGAEGPLLSAWT"
     gene            10165..11112
                     /locus_tag="VKU03_05990"
     CDS             10165..11112
                     /locus_tag="VKU03_05990"
                     /inference="COORDINATES: protein motif:HMM:TIGR00765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLJ70849.1"
                     /translation="MTILGIRRRRPKPGRAEAKPCGVAPQSDNVRSETLLQIATNVVM
                     RISRDSLMLVAAGIAFYAMTAIFPAIAAFVSIYGLFANPTAVESQIAGLSSLLPAASL
                     KLLTDALQSFASKSNSTLSLALLVSVAVALWSAKASMTALMSGLNIANETIEKRSILV
                     QQATGLALTLGGIVFAGVALAAIALLPVIIDIMPLSATVKSALGLGRWPLLAVLICFA
                     LAVLYRFGPYRERAKWRWITLGSAIATVLWLIGSGLFSIYVSRFGSYDKTYGSLAAPV
                     VLLLWFWLSALVVLLGAEIDAEMEHADGKAARPLPDGAP"
     gene            11214..11675
                     /locus_tag="VKU03_05995"
     CDS             11214..11675
                     /locus_tag="VKU03_05995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70850.1"
                     /translation="MIPLRIRPHVVALAAVALGGLAPGAALGAQSGGEQAFDIHSGQT
                     LTFAAKVEGGKVIVGRPRVSRLGAAHPAAGEMTVDLTPRDKDMYEQVIVNEKTSQPID
                     FVATALVGDVKIDERVLCGRLDGPASSRIGANHWRVRLHDFEVGKGDGSCD"
BASE COUNT         2171 a         4056 c         4233 g         2109 t
ORIGIN      
        1 cgctggtcga gttgcatggc ggctcgctgc ggatcaattc gcggcccggc gacggcgcgg
       61 tcgtgctcgt cgcgctgccg cgctcgccgc gcgagcatca caagctggcc ttggccgtcg
      121 gggcgtaaac cgttattgcg aagagcgaag cgacgaagca atccattcct atggcgacgc
      181 gtacgaatgg attgcttcgc tcgcaatgac ggctgttggg ctgtcagaag tgataggacg
      241 attgcgcgaa agcctggaag cgctgggtgc tgaacgtcgc gacctgggcc gcgcccgatg
      301 gcgtcgcgtc ctggaattgg acctggccct tcgcgctgag cgcccagtag cgcgcgccga
      361 ttccgatgtc gaaattgggc gacagcgcgt aattgacctc gccctccacc tggaatcccg
      421 tatggccgat tccggtttcc ggcagcgggc cgaaaaagtc cgtcggcgtg cgcagccaat
      481 ggtcgttttt gccgttgaag aacgtgatcg gcagccaggc cgcctcggct gcgatggtga
      541 agccgcccgg caggttcgcc ttgccggcga ggccgaggcg cacggagcgc cagatattgt
      601 cgtcgctgat cgtcagattg ctcgccgaga ccactcccgc cgggcatatc agcgggttag
      661 aggcggtctc cgtgcagccg aacgcgttaa aacggtcttc gagataattg aagccggcga
      721 ggccgcccag cttgtaccat gaagtcccga aagcgtagta gccggcgtcg aacgtcgcgt
      781 agccaagacg gccatattgc tgcgggctgt tggtcgagga ataggggctt atgcccggcg
      841 gaaagtcctc gtcctggagg ttgccgccgc tttgccggcc gatcccggca aagcccttga
      901 tgaacacgcc ggaactgtcg tcgatccgtc caaacacctc gcccgtcacc gccgacggat
      961 catgataggt cagccgcgac aggaggactt gtccgctcga atcataaagg ttcatcttcg
     1021 cggagccgat cgacatgccg atgcgctcgc cgaatgtcgc ggtgatgtcg ctctgggccg
     1081 gctgcggcga atagccggac tcgcccgcgt cgccgtcgaa ttcgtagttc aggccgaggc
     1141 gcagcacata gcccttgaag gcggcgctgg tgtcgctttg cgcgctgatc gcgaaattcg
     1201 gcaggcccac gacctcgctg cgcccgagat cgtaatagag agcctctgcg gcgaccgacc
     1261 agttgcggtc gatggcatat tcgacgcccg cgccgacggc gaagccgatg ctcaaatcgc
     1321 tatgcgatcc gctatattga gcgccggtcg caaaggacgt gttggtcgaa agactcgtct
     1381 ggccgaaggc gaagccgccc gtcgcataga tcaaggcgtc gtcgatcggg gcgaagccga
     1441 tcttggctcg taccgttcct aaagaccgca attgctgcga ctgcgatacc gtgaacggcg
     1501 ttccgagata gaggccagac gcgctggcgc tcccgcgcga ggcggcgaga tcgtaatcgg
     1561 cgacgccgcc gaagacgaac gagccccatt gcttggaata gcccatctgc acgccgccga
     1621 gaaatccggc cggcgacagc gtgaaggcgc ctggcaggcc ggtcgtcggc gcagtcgccg
     1681 ggaaccaggt catcgtttcg ccgtcgccgg acgtgaacag gccgccggca tgcaggccgg
     1741 cggtgaagcc ttgccagcgc ggaccggcgt agggcgccga ctcaggcgcg gccgcgtcga
     1801 gatcggcggc ttcggcggcg gcgacgccga tcagcgcgaa agcggcgaca agagcgaagc
     1861 ggcgcaagat ggacgtcccc ggcgcggcgc gcttcaacaa cgcgaaagcc gcgtaaggag
     1921 acgttaaccg taacatcctt aacaaattgc tgctcgcgct gcgccgggcg ccgcggcttc
     1981 ggcgctagcg aagcaaaaat cgtcaaccat tccagcaaaa tgccggccgc gagcggccgc
     2041 gttaacgtcg gcgacgggcg cgaattaacc ttgctgggcc gctgtcggcg cgtcgagctg
     2101 aaagaacagg gccgctcgcg gccgcgcatc gcgccgctgc aatttgagta aaatgccggc
     2161 ctttcaatcc gttgcgccgc cgcccgcggg cgcgccgccg ccgccgttac cgtcccgtca
     2221 aggataaccc ggcggtcacc tcggtaaatc cgaatggctc gaatccgagg ctttttcttc
     2281 gaaagccgct gaaacactcc tggcatcgtc cccctgctca gaacaggcgc gaagcaaacg
     2341 cgccggcgaa tgggggcgtt gatgaaaacg gggttgcttc gggttcttgt ctcggtctgc
     2401 ttgatggcgg cgctcttcgt tgcgaggacg atcagcgcgg aagccggctc tctgccgcgc
     2461 gccagctacg ccgcgatcgg cggcgcgacg gccgtaccgt acggctgggt tgatttctgc
     2521 catcgagaac gcgccgaatg cctcgggccc gttcttccgg cgctcgacat caagctgacc
     2581 aatgcgacct gggcgaagct cgaccatatc aaccggatgg tcaaccaggc gatcacgccc
     2641 gtcaccaatc tcgaacattg gggcacgatg ctcgatcatt gggactatcc gctcgacggc
     2701 aagggcgact gcaaagttta cgcgctctat aagcgcaagc ttctcatcga tatgggcttc
     2761 ccgcgccagg ctttgctgat gacgatcgtc cgcgacctcg acggcgaggg ccatacgatc
     2821 ctgacggtca agaccaatcg cggcgagttc gtgctcgaca atctgagcga cgagattcgc
     2881 gcctgggacg ccaccggcta tcgctacatc aagcgccagt cgcaagaaga cccgaatgtc
     2941 tgggtcgccg tcggcgccga cgcgacggcg caggcttcgg ccgcgacggc tgcgaacgag
     3001 taggggacga gaaagagccg gcgttcgcgg ttccgcctca atcgggccgg ctctccgccg
     3061 cctggcggta atatcgactg gtttcggcgt gccagagtcg tcgcgactcc aaatgcgcca
     3121 ggccaggcgt cgcggctggc aaccgcagat tttcgaccag gctctcgatc tcgcgccgcg
     3181 ccgcctgctg cgaggcggtg agcgcgcggc tctcggcgcc ggcctcgatc ggatcgagcg
     3241 cggtgaggcc gatcgggaag agttcgcgga aaatcacccg gtcgtgcaag ccgtcgacgg
     3301 cgcggaactg caattcggcg ccgagcttgg cgatgctgat cgcgatctgc ttggcgttgt
     3361 tggaggcgag cgaggcgata cggttgcgcg tcatcaccca atcgatcacg ccattgtcga
     3421 caaggcggcg cttgcgccgc gcctccagga cgagatcggc gtattgggcg acggcgccgc
     3481 gctgcgcgcg gtcatgggtg acgcgcgaaa agacgtcgac atcgatgaaa ctgtcgttga
     3541 gcggcgacac cagcgtgtcg gcgagcgaat gagcgagccg catcaggtag ctgtcggacg
     3601 ccggcgtgtc gatgacgacg aattcgtatt cgtgctccac cgcgccgatc gcgtcggcga
     3661 aaagcgcgaa ttccttggcc tcattctgcc ggacgtcgcc gctttcgccg cgctccagcg
     3721 cgcaatgaaa aggcgtttcg atcggccagg aggcgctctg agcccaggag cgccgattct
     3781 cgaaaaatcg agtcagagtg cgctggcggg tgtcgagatc gatcgtcgcg acgcgccgcc
     3841 cggccttcag aagcgcgacg ctgagatgga tcgacaaggt cgacttgccc gccccgcctt
     3901 tttcattgcc gacgacaatc acgtgggcgc tgcgcaactc gggctgatcg tcgtcaaagt
     3961 gcatgtaaag ccaggcgaat cgtctgcgcg tcgtcttgat ttcgagacat agtggttttt
     4021 ccttcttccc gcaaagcggg gagaaggtgg cgcgaagcgc cggatgaggg gctgcgcagg
     4081 actcgaaggg tacgcgctcg atgaagacta atccgcaagc tcggcgccgc ccctcatccg
     4141 gccgagcttc gctcggccac cttctccccg tttcacgcag ggctgtccgg ggaaagtctg
     4201 ggaccattcc ggcgcgtgaa gaatctcgcg tctcggattg ctggcgccgg cggcgccacg
     4261 ccgaccgatc gccgtcaacc cgccatagcg cctacaaaag tacgcgcaag tcgatattcg
     4321 cttgtgcggc gctatggcgg gtgctccact tcgttccgcc ccttcgggtg actgcgatcg
     4381 gccggcgtgg cttattggct gccaagacgt tttgctgccg aaaccggccg ttcggccggt
     4441 tcgaacagca aaacgtcttg gtagcaaaaa ggccatcgac gcgcggaaga gtcaacattc
     4501 gcggagcgac cggcgaagcc ggcttgatgt tgacgcttcc gcgcgtcgat ggcagcggcg
     4561 taggggattt tgcgtggtcg ttgcggccgt ggttactgca agggtttgcg aaacaatttt
     4621 gatgatcgcg ctggtcttct gagtctcgcc aaccagaaac ttgacgataa aatctctaaa
     4681 ctgctcgtgt tgcgaggtct tcgccaacga cagcacgact cgcgtctcgc tttccccgga
     4741 cagccctgcg caaagcgggg agaagggacg ggatcatccc cgcgttggct cggcgtggcc
     4801 gatcacccgc gcaatatcgg gaaaatcgtc gcgcaccgtg cgctccagcg catcgacttc
     4861 ctcatgcgtc gcgctgaccg aaacattggg gtcgatccgg caatggaaat tgaccaggag
     4921 accgcccggc gtatggcgcg cgcgcacgtc gtgaatttcg cgcaggcggc cggtttccgc
     4981 ggcgcggcgc gccaggctgg cggcgatcgc ctttacgatg tccggctcgg cttcgcggcc
     5041 gggaatctcc tcgatcgcca tcggctcgat atgcgtctcc acctcgatgt ccgggccgat
     5101 ctcgcgcttg atcgcggctt cgagccggct ggcgatctca tgggcctcgc cgaggctcat
     5161 ttcgccgtcg atctccatat cgagactgac cgagcggcgg ccctcgatct cttggatcgt
     5221 cacatgatgg accggcaggc ggcgccggcc ggcgatcagc aggacccgtt cgagcacgct
     5281 ctcattgtcg agcgcgcgcg gattggcggt gacggtcagg gccgcctgcg gctcggccag
     5341 agcgatcttc tgcaggatgt ccgtcttgat cgtcgcgacg cgctcgagcg gcaacgtgcg
     5401 cgagacgaac acgccgacct cgccgatcgt ttgcgcgccg ctgcggcgca gccgcacata
     5461 atcgacgccg gcgacgccgg ggacggcctc gatcagcgcg ccgagccgct tggccagccc
     5521 ttgcggcgcg gtgtcgacca atgtgtcgat cgtgcgcctc cctaagcgat agccggcgaa
     5581 ggcgatgaac aaggcgacgc cgaccgccgc gaaggcgtcg gcattggcga cgcccgcctc
     5641 ggtcgcgacg aggccggcca ggaccagaaa cgaggagacg agatcgctcg aaaaatgcag
     5701 cgcgtcggcg gcgagcgcgt cgctcttcgt atcggcggcg atcttggcga ggccgcgcca
     5761 gcgcgccgcg tcgacggcga tggcgacgat caccaccgcg aaggcgaggg gggtggcgtg
     5821 gacgatcgtc tgcgcctcgc cggcgatgcg gcgcacggcc tcgaacgcga caaaaaaggc
     5881 gaggaccgcg agcaggccgg tctcggcgag cgccgccacg gcctcgaact tggcgtggcc
     5941 gaacgggtgc tcctcgtcgg ccggcttgtc gccttcgcgc accgcgaaaa aggtcaggat
     6001 cgtcgcgccg acatcgaggg cgctatggcc gccttccgac gtcagagcca gcgagccgga
     6061 gaggaaaccg gcggcgagct tggcgagggc caaggcggcg ctggcgagga tcgagaccaa
     6121 ggcggcgcgc tgcttgcggc cggcggcgtt catcaaggcg ctcccttgca tggcgaccct
     6181 ctttcgccgc tcatcgccgc tggttatctt ccgcgccgca caaggacgca atcggcggag
     6241 aaacgatcat ggaatatctg cacacgatga tcagggtcac cgatctcgat caatcgctcg
     6301 atttcttcgt caacaaattc ggcctcgccg aggtgcgccg catcaataac gaaaaaggcc
     6361 gctacacgct ggttttcctg gccgccgacg aggacattga ccgcgccaag caatcgcagg
     6421 cgccgctggt cgagcttacc tacaattggg accccgaagt ctataccggc ggccgcaatt
     6481 tcggccatct cgcctatcgc gtcgacgaca tttaccgctt ctgcgataag ctgatgaaag
     6541 ccgggatcac gatcaaccgg ccgccgcgcg acggccatat ggccttcatc cgcacgccgg
     6601 acaatatttc gatcgagctg atccagaagg gcgaggcgct tccgccgacc gagccgtggc
     6661 gctccatgcc gaataccggc gtctggtagg agaaccggcg ccgaaagccg tccgctcttg
     6721 cgctttttgg cggatcggcg cggaaagccg cgccttgggc gggcggcaag gaaccaaaac
     6781 aggtcacgcc ggttgatgac cattggcgtc atcggcgggg tcatgtcatg gcggatgagc
     6841 gttctttgga cgaattgcgg cgcgacgccg acaaggcgcg ggccgacctc gtgcggacag
     6901 tcgacgagtt gcgggcgaga gccagtccaa ccgggctgat gagcgcggcg gcccgctcgg
     6961 cgcgggacaa tccgcttcag accgtcgcgc tcgccgcggg cctcgcttat ccgcttttga
     7021 aaaccgtccg gtcgatcccc gcgccgctcc tgatgatcgg agcgggattg ttcttgtcgg
     7081 gcaccagcgc cgggcggtcg gccagccgca aagctgtggc gttcggctcg gacatgacca
     7141 ataaggtcgg agctctcgcc tccgacatga gcaagaccgc ggccgagcgc gccaatgccg
     7201 gattggcggc ggcgcgcgac gcggcggcgg ccgctggcgc gaccctttcc gacaaaatga
     7261 gcgccgtcaa agagaaggcg agttcgctcg ccgccgcttc cgccgatcag ttgcagcgcg
     7321 ccggcgcggc gaccgccgcg gcccaatcgg accttgccgc ccgcgccgcc gacgcggcga
     7381 gcggcttctc gccccaggcg agcaaggatc aattgtcgcg ccttgtcgaa gaggccggcg
     7441 cggcggcggc cgatacgatc aggcgcaatc ctcttcttgt cggcggcgtc ggcgccgcgg
     7501 tcggcgccgt catcgccggc cttctcccct cgtccaaaac ggaggaccga tgggtcggcc
     7561 cggtcagcga tcgcctgaaa agcggcgcgc gggaaacggc gtcgcaaggc tatgaggcgg
     7621 cgcgggacgc cgctgcggcg gcctacgatt cggccgcgga gcgcgccgga caggaagggc
     7681 tcgacgctga cggcgtgaaa gcggcggccg aggatttcgc cgagcgcgcc cgcaaagtgg
     7741 cgaacagcgc gctcgacgcg gcggcgcaag ccgaaaaagc gcattgagcg aaggagagga
     7801 gcattcgcat gagcgatcca ggctcattcc gggaaaacga ccccaattcc ggcgatctgg
     7861 cggatcaggc cgtcgccgcc ggcaagcagg ccaaaaacgc cgccgcggac tttgcccaga
     7921 cgacgatgga cacggtcaag cgccgcgcgg gcgacgccgc cgacgcggcg aaggacattg
     7981 ccggccaagc cggcgagcgc ctgcaagagg aggcaatggg gaaaaaaggc gaagcggcgg
     8041 attatgtcag tcgcctcgcc gacgcgatgc gccgcgccgc gggagaattc gagtcggatt
     8101 tgccggccgc ggcgtcgtac attcgcagcg cggccgacca ggtcgaaagc gtttccgaat
     8161 cggttcgcaa cggcgacttc aaggatttgc tcgaaggcgc ccagtccttc gcgcgctcgc
     8221 agccgaccgc ctttgtcggc ctcgcggcgc tcgccggctt cggcctggtg cgcttcctga
     8281 agagctcggc gcacgagacg ccgaaagccg acgacgcggc gcagacgcgc tacgactatc
     8341 attgagaggg gaggggacat gtaccctttt tccactgacc gctcgatcgg ggaattgctc
     8401 ggcgaagcgc tcgccgagct ttccaagctg atccagactg agttcgcgat cgccaggacg
     8461 gaaatgtccg agaaggtcgc caagatgctg ggcgcggcga agctgatcgg cgttggcgcg
     8521 gcgatcgccc tggccgcgct gttcatcatc ctgatggcga tctcggcggc gttgatggcg
     8581 gccgggctgc ccggctgggc gtcgtatctg cttaccggcg gggtcgccgc gattatcggc
     8641 gtcgccctga tcatggccgg agccagccgc ctctcaagcc ggtcgctgaa gcccgcgacg
     8701 acgctcgatg agctgcggca ggaccgcgta accgcaaagg cgatgacgca atgagcgcgc
     8761 ccgaaaaaat ccagcagagc ggcggcccgt ttctcgaaag cttgcgcaat gcggcggctg
     8821 aaaatcccgc cgcggcggcg ctgatcggcg cgggcgtgct ttggctgttc gccggccgcg
     8881 tgcagattgg gccgatggcc aaagccggcg ccgcccgcat cgccgacgcg gccgcggagg
     8941 cgcagcgcgc gacgagcgac gcgatggctt cgacctggcg ctcggcgaag gagaatgtct
     9001 ccggcctggc gagccgcgct gcggcgcaag tcaagcgagc cggcgacgac gccggcgagg
     9061 ccgccccgcc cgacctccct gagccccagc gagagagcgg cgcgcccggc gcgccgatgc
     9121 aggagcagcg cgcggcgata agccggcgcg tcgccgatgg gctccatgcg ggggcggaga
     9181 cgttctcctc ggcgcgcggg caattgagca acgtgctgca gagccagccg ctggcgctcg
     9241 gcttgatcgg cttggcggtc ggcgcggccg tggccgccgc gctgccttcg acgcgggtcg
     9301 aaaacgagac gctgggcgag accagcgatg cgctgcgcga tcaggccaag cagcagatgg
     9361 atcgcgccgc cgacgccgcc tccgccgctg cgacgaaagc cacggaggaa gcccgcgcgc
     9421 agaacctgac cgccgacgcg ctcaaggcca aggtcgaaga gaccgcgacg aagatcagcg
     9481 gggtcgccga cagcgcggcc gagacgctgc gcgacaaaat gcggcctgcg ccgtgaatcc
     9541 gggatcggat cgaatccccg gtctggagga tgcgaagcgc gcggtggagg cgctgaccgg
     9601 ctggggccgg ccgaccggcg acgcctcggc gctggttcag ccgcgaagcg ccgagacctt
     9661 ccgcttcggc gatgacgggg ccattccgaa ccatccgttt tggccgctga tcgtctatcg
     9721 cgccgcggtc agattccccg gcaagctcga tcccgccgcc gtgctggagg atctcttcca
     9781 caagaatgga tggggcggca tgtggcgcga cggaatttat gattatgcgc actaccattc
     9841 gcagattcac gaagcgctgg gcgtggcgcg cggcgcggcc gaagtcgagt tcggcggcgc
     9901 cggcgggcga attctcccgc tgaaggccgg cgatgtcgcc atcctgccgg cgggggtcgg
     9961 ccatcggcgc gtcgcggcga gcaaggactt tttggtggtc ggcgcctatc cgccggccgg
    10021 ccaatatgac gaatgcgcca agcccgagga tcgccagggc gcgctcgcga gaattccggc
    10081 gacgccgcgc ccgctcatgg acccggtcta tggcgccgaa gggcccttgc tcagcgcctg
    10141 gacataaaag ctcgcggggc gatcatgacg atcctcggta tacgccggag gcgtccgaag
    10201 ccgggccggg ccgaagcgaa gccttgcggc gtcgcgccgc aatccgacaa tgtccgctcc
    10261 gagactctgc tgcaaatcgc caccaatgtc gtgatgcgga tcagccgcga cagcctgatg
    10321 ctcgtcgcgg cgggcatcgc cttctacgcg atgaccgcca tttttccggc catcgcggcg
    10381 ttcgtttcga tctacggcct cttcgccaat ccgaccgcgg tagaaagcca gatcgccggg
    10441 ctttccagct tgctgccggc cgcctcgctg aagctcctga ccgacgcctt gcagagcttc
    10501 gccagcaaga gcaactccac cttgagcttg gcgttgctgg tttcggttgc ggtggcgctg
    10561 tggagcgcca aggcgagcat gacggctctg atgagcggcc tcaacatcgc caatgagacg
    10621 atcgagaagc gcagcatcct cgtccagcag gcgaccggcc tggcgctcac tttgggcggg
    10681 atcgtctttg ccggcgtcgc gctcgccgca atcgcgcttc tgccggtgat tatcgacatc
    10741 atgccgctca gcgcgaccgt taaatcggcg cttggcctcg gccgctggcc gcttctggcg
    10801 gttttgatct gcttcgcctt ggcggtgctc tatcgcttcg gcccataccg cgagcgcgcg
    10861 aaatggcgct ggatcacgct cggctcggcg atcgcgaccg tgctctggct gatcggctcg
    10921 ggactgttct cgatctatgt ctcgcgcttc ggctcgtacg acaagactta cggctcgctg
    10981 gcggcgccgg tcgtgctgct cttatggttc tggcttagcg ccctggtcgt gctgctcggg
    11041 gcggaaatcg acgccgagat ggagcatgcc gacggcaagg cggcgcggcc tttgccggac
    11101 ggcgcgccgt gaggatcggc gggcgattcc ggagcgctca ttcgacgcgc tgacgagagc
    11161 cgcccttccg gtctaaaatg gacgccttgg cacgatcctt ggaaggcgcc ggcatgatcc
    11221 cgctccgcat ccgcccgcat gtcgtcgcgc tcgccgctgt cgccttgggc ggcttggcgc
    11281 cgggcgcggc gctgggcgct cagagcggcg gcgagcaggc cttcgacatc cattccggcc
    11341 agacgctgac tttcgcggcg aaggtcgagg gcggcaaggt gatcgtcggc cggccgcgcg
    11401 tctcccggct cggcgcggcg catcccgccg ccggcgagat gaccgtcgat ctgacgccgc
    11461 gcgacaagga catgtacgag caggtcatcg tcaacgagaa gacctcccag ccgatcgact
    11521 tcgtcgccac cgccctggtc ggcgacgtca agattgacga gagggtgctg tgcggccggc
    11581 tcgacgggcc ggcctcctcg cgcatcggcg ccaatcattg gcgcgtccgg ctgcacgatt
    11641 ttgaagtcgg caaaggcgat ggaagctgcg actagccggt cattgcgagg gcggggaaaa
    11701 tccgttcgca cggcgctcga aatcgccgag atcgagaatg cgcttcagct tgcggggggc
    11761 ttgcgaaacc ttcttcgctt gcttgccgtc cgcccgcgac gcgcgacgag cgatcgatag
    11821 atgcatcgct cggcctcatg ctggcgcggc gggaactacg ggcgcgtggc gcgaaactct
    11881 tgagcggtac gaaaagacat tgacgcaaga attggcaatt tgccctcgat ccgcacaaaa
    11941 agactgtgtc tcgccaacgg cgccgcaaag cctccgccaa gctttttgca aatttttgtt
    12001 tcgccgtttc gtgcgatttc aatgtgttag cgcacgaaaa agtttggaaa cgagttttta
    12061 ggtccttttc gattcgccgg cgctcgcttg cccaatttta atgggaacgg accggcttag
    12121 gaagaaattg tttagatgtc tttttagctc atgccaacca ccgccaagaa gaccgcggcg
    12181 ccgaggactc ctctccccgc tttagcgggg agaggtacgg agaggggctt gtcgcgtatc
    12241 tttgacggtc ggcgctttga gcaaccggcc tgtctccgta cctctcactg ttttacggcc
    12301 agcgcccttt gccaagacca gcccctctcc ttacctctcc ccgttccgct tcgctccacg
    12361 gggagagggg cgtcggcgcc gcggctcgtt ttcgagcgcg gggatggcgg gcgcgacgct
    12421 tggatcttct ctccccgcga agcggggaga ggtacggaga ggggctggtc gccttgccag
    12481 gacggctgag gataaccgac cagcccctct ccttacctct ccccgctccg cttcgctcca
    12541 cggggagagg ggcgtcggcg ccgcggctc
//
LOCUS       DATGJW010000196        18499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1486683, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000196 DATGJW010000000
VERSION     DATGJW010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 18499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18499
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1486683"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..771
                     /locus_tag="VKU03_06000"
     CDS             <1..771
                     /locus_tag="VKU03_06000"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70851.1"
                     /translation="LVSLGASCFVGLGAYIAFGVLNKLDAPLPLALAAAGLGGGALAA
                     LVSPALFRLRGLYFTVGTLALAELLRLTMINVSAFGGATGIFLKNDPPDLPILFRYAL
                     AVALLASLAMTFCARSRASVILRAVRDDEDAAAQMGVRPFRVKLAAFVLASAVAAMAG
                     GVQAVKLGAIEPYGMFGLQWSIDVLTMAIIGGVGLRLGPLVGAVFVIALGEALADYPE
                     AHVAIAGVILIVLIRFAPRGVVGFVANPALVLRRPAPA"
     gene            768..2327
                     /locus_tag="VKU03_06005"
     CDS             768..2327
                     /locus_tag="VKU03_06005"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ70852.1"
                     /translation="MSAPPLLEVSGLSKRYGGLVAIDHLDLSAPAGAITGVIGPNGAG
                     KSTLIGLIGGALAPSAGTIRLDGKDVTRLPASERARAGIGRTYQIPRPFLDMTVEENL
                     EVPQYSIAPFIGAAAAREEREALLVRTGLADAAALPARALPLLRRKRLEVARALALKP
                     RLLLLDEVGAGLVDSEIDELIALIQSILDKSTAIVIVEHVIRIVRECCARSLVLNFGK
                     RLIEGPTSEILANDQVAAVYLGTGGAHRKAATGAETSRADGAIHVAAPAEERRKLAPL
                     VAPAADSKEPILELRNVAAGYGQARVLKGVSLALAEGEAIAILGANGAGKTTLARVIC
                     GAVAPSAGILRIGGRAAGLAPHRVAALGVAHCMEGRRIFSSLSVEENLRIAARGADAA
                     TARQRLEAVYDLFPVLFERKAQVGTSLSGGQQQMLAIARALMARPRLVIFDEISLGLA
                     PVIMDKLYEALAQLKQARMTLILIEQDVDRALALADRAYVLEQGVFALSGAARDIAAD
                     PRLRRVYMGAA"
     gene            2418..3422
                     /locus_tag="VKU03_06010"
     CDS             2418..3422
                     /locus_tag="VKU03_06010"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR substrate-binding domain-containing
                     protein"
                     /protein_id="HLJ70853.1"
                     /translation="MARRYLDQRLRLHLLRAADAIEAQGSLIKASAALGVSQPALTKS
                     LHELEAILQLRLFDRHSRGVSPTEAGVAFVQCARRVLAELHRLDEQLDRLSSLEGGTV
                     AIGALPVAAAGLLPGVLTRLRAAHPAVRVRLLQGRTRELLPLLASGEIDLIVGRLYEP
                     AVPDGFIREALWTEPISILARAGHPIFSEPVTVEALRRYDLVLPTITQRVGEEVEGLI
                     AQLGLDAAASLRSSSYSFIREMLHGTDLLAIVPRLMMVGDLLRGDLRVAPLPLAAPDR
                     PAGLILPRGRPLPAAAEFLATSLRAYVAEIAQLGLAAPITASDGAGARSDGTRDATPA
                     "
     gene            3465..4193
                     /locus_tag="VKU03_06015"
     CDS             3465..4193
                     /locus_tag="VKU03_06015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HLJ70854.1"
                     /translation="MSESANKTALVVSAHSADFVWRAGGAIALHAERGFAVTVICLSY
                     GERGESAKLWRQQGMTLDRVKTERRKEAEAAAAKLGAADLQFWDLGDYPITLSEESLC
                     RLVDVYRAIHPLFVLSHSRRDPYNRDHPAVSDFAQHARIVAQAHGHKPGERVLGAPPV
                     FLFEPHQPEQCGWKPDVLLDITSVWEKKRAAIECMAGQEHLWDYYTRVAQQRGVQAAR
                     NSNKKIAYGEGYEAVFPHVVESLA"
     gene            4190..4888
                     /locus_tag="VKU03_06020"
     CDS             4190..4888
                     /locus_tag="VKU03_06020"
                     /inference="COORDINATES: protein motif:HMM:NF006731.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047443 -
                     4-hydroxy-4-methyl-2-oxoglutarate aldolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-carboxy-4-hydroxy-2-oxoadipate
                     aldolase/oxaloacetate decarboxylase"
                     /protein_id="HLJ70855.1"
                     /translation="MSAGFVFTDPQRVAPELAAAFAPYGVATIHEAQGRAGLLASYMR
                     PIYPGARLAGSAVTVSVPPADNWMLHVAVEQCRAGDVLVVAPTSPCDAGYFGELLASS
                     LVARGVVGLIIEAGVRDVAALTKMRFPVWSKAVSAQGTVKETLGSINAPLVCAGQLVH
                     PGDLIVADDDGVVVVPRLNAEDALRKSEARERKEAATRARLQAGELGLDIYAMREKLK
                     EKGLVYRPFADREG"
     gene            4890..5816
                     /locus_tag="VKU03_06025"
     CDS             4890..5816
                     /locus_tag="VKU03_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70856.1"
                     /translation="MAPGATNAEAGAPALRAAIGAYKHTQALKSGAVSSSKLRLDFAD
                     VSPISRAFAPMVRELRFDVCEMAIATFLQAKAYGKPLVLLPIVAAARFQESALLCRAD
                     SAIRGPADLAGRRIGVRAYSQTTGMWLRGILAEDFGVRPEQARWITFEDAHVAEYRDP
                     PWAERAPPSKALLAMLHAGEIDAAILGNDVPDDPGFRTVFPDPFAAGEAFRRRRGFVP
                     VNHLVTMRRDLADARPDLAAEIVGLFKEAKATANLSPAQQDALPTTRAELRAAVALAS
                     RYSADQGLLPRRLSEDEAWEGSPDLDGRASRL"
     gene            complement(5856..7370)
                     /locus_tag="VKU03_06030"
     CDS             complement(5856..7370)
                     /locus_tag="VKU03_06030"
                     /inference="COORDINATES: protein motif:HMM:NF012363.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxylesterase family protein"
                     /protein_id="HLJ70857.1"
                     /translation="MTPGTVSTTDGLLALPEPNAAGVRCFKGVPYAAPPVGPLRWRPP
                     APVTAWRGIRRTDRFGPNSLQGIVFDDIDPRPAGVSEDCLYLNVWSGAVGSGDLLPVM
                     VWIHGGGFVVGSGSEPRYDGSNLAARGIVVVTLNHRLNALGFLAHPDLSAESPHRGSG
                     NYGMLDLVAALGWVKRNIAAFGGDPKRVTIAGESAGSMAVSGLMASPLAKGLFARAIG
                     ESGGMFASPGHPILSLAEAEEAGRAFARKLGAASLEALRAAPAEDILAAAPGLGFRPI
                     IDGYFLPRPPAQIFAAGEQSDAPLLAGWNRDEGFNFSLLHGAPDDQSYPALVRGIFGG
                     QTAEALRFYPSGDAETDKASAKALGGDLSIVHGTWAWLEAQRTSGTAPIYRFRFDRCP
                     LTPEGWFGSTPSAEAGAFHAGEIFYVFDNLDACPWRIAADDRALATLASAYWVNFIKS
                     GDPNGAGLPKWPSYRDGERPFLVLDASPKVRMEHETAKHEFLARATQTRAEDTG"
     gene            complement(7473..8624)
                     /locus_tag="VKU03_06035"
     CDS             complement(7473..8624)
                     /locus_tag="VKU03_06035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006641587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ70858.1"
                     /translation="MDEPYAEIRQAVAKLCARFPGEYWRDLDRRMAYPTEFVNALTEA
                     GYLSILIPEEYGGAGLPLSAAAAVLEEIQRAGCNGGACHAQMYTMGALLRHGSEDQKR
                     RYLPAIAEGELRLQAFGVTEPTSGTDTSSLKTTARREGDHFIVNGQKIWTSRAEHSDL
                     MLLLARTAPQSAGAKRSDGLSVLIVDMRAALGAGLSIRPIRTMMNHATTEVFFDDLKA
                     PAENLIGEEGKGFRYILSGLNAERILIAAECVGDAKWFIDKASAYAKERRVFDRPIGQ
                     NQGVQFPIAKAYANMRAAELMTREALRLYEAGANPGAEANMAKMLAADASFEAANVCV
                     QTHGGFGFAEEYDIERKFRETRLYQVAPISTNLILSYLAEHVLGLPRSY"
     gene            8836..9666
                     /locus_tag="VKU03_06040"
     CDS             8836..9666
                     /locus_tag="VKU03_06040"
                     /inference="COORDINATES: protein motif:HMM:NF014373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HLJ70859.1"
                     /translation="MDLRSFLGEPTGQTALQLAELGLAFILSALIGLEREIRQKSAGL
                     RTYTIVGFASALIIVVSKYGFTDVLEQNRIVLDPSRIAAQIVSGIGFIGGGVIFVRKD
                     LVRGLTTAATIWLTAAVGMACGAGLPILAIAVTGGHFVVIGLFPAVERRLPKSRWAPS
                     QLHISYRGGQGVLREVLAICTQHDFAVSRVQVEGDPSPRRKQSGGQQAKGHDMQWDDE
                     ASSFEETPASEAGSDGNGIVTLLVELRGTKSIPKLAARISEIPGVVSVQAGDANFLSE
                     "
     gene            complement(9673..10062)
                     /locus_tag="VKU03_06045"
     CDS             complement(9673..10062)
                     /locus_tag="VKU03_06045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLJ70860.1"
                     /translation="MAALPFRKTASGGIEILLITSRETGRFIIPKGWPMKRLSDSDAA
                     AREAYEEAGVVGKVRRKPIGEYRYWKRLERSFELLAVDVYPLEVETQRADWPEKNARR
                     IGWLPQEDAATLVDEPGLVTLIRNFKV"
     gene            complement(10310..11059)
                     /locus_tag="VKU03_06050"
     CDS             complement(10310..11059)
                     /locus_tag="VKU03_06050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HLJ70861.1"
                     /translation="MRILVVNPNTTAAMTRKIGAAARAAASPGVEILAVNPDSGPPSI
                     EGYYDEVFSIPGVIEEMRQAGDVDATVIACFDDTGLDAARCVSKAPVVGIGEAGFLMA
                     SLIADRFGVVTTLPRSIAPIEKNLAKYGLASRCARVRACQVAVLDLEDARSNARRLIL
                     DEARKSLVEDGAEAIVLGCAGMTELARDLSEETGAPVLDGVACAVALAEAAARIGLRT
                     SKLNTYAAPLPKAYAGLLASFSPSASAAKMR"
     gene            complement(11074..11796)
                     /locus_tag="VKU03_06055"
     CDS             complement(11074..11796)
                     /locus_tag="VKU03_06055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008878388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70862.1"
                     /translation="MQPMLTFANVELFYDHIYALKGVSLQLMEGETVALIGANGAGKS
                     SILRAITGLRKIRSGQIHFEGRRIDGLGPEAIVGLGIAMVPEGRRVFPYMSVRDNLLM
                     GAFSRKDSAGVRATMDMVLARFPRLQERYRQAAGTMSGGEQQMLVIGRALMAKPRLLL
                     LDEPSLGVAPKIVQDIARSIVSINRDDKVSVLLVEQNSRMALRVSHRAYALTTGSVAL
                     TGASADLVKDARVQQLYLGGDL"
     gene            complement(11800..12579)
                     /locus_tag="VKU03_06060"
     CDS             complement(11800..12579)
                     /locus_tag="VKU03_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70863.1"
                     /translation="MAPILAISGITKQYGGLVAVNDVSFEVEQGEILSVIGPNGAGKS
                     TLFKLIASFVKPTRGEVRFRGERISGLAPHTVARKGVVRTFQETTIFKGMTARDSVII
                     AHHLRSKAGLAGFYLGTAAARRDEAEFGRSADEILDFLGLRAAANEIASNLPQGSLRA
                     LGVAIGLAANPTILLLDEPFAGMNHDETRRAVEMTRAVRDRGVTLLLVEHDMAAVMKI
                     SDRIVVINFGAKIAEGTPAEIQKNEKVIEAYLGVEDETIGV"
     gene            complement(12582..13583)
                     /locus_tag="VKU03_06065"
     CDS             complement(12582..13583)
                     /locus_tag="VKU03_06065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007879286.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70864.1"
                     /translation="MSRPAQLVALAVFLLAVFVAVPAAIGLTHRTDFYYTLSSVALLA
                     IASSGVWLTFYIGRINIGQGAYALMGGYVSAILIVRCGLSFWVSLPVAGAFCAAASVL
                     IGLPILRLRGVYFAMVTLVLTEVVRLLAMALSITNGAKGMVSIPPPGPIALFGVTLIP
                     SFDVFVERNEAFYYLSVTIMCLCFAALYRLVNSRVGRLFVALQQNEELASSMGLNVPA
                     LRVLAYSISSFFGGIGGAIFVAIAQSIYPSSFTVADSINFMLNCFLGGLGYVFGPMLG
                     TLVLYFGWDLLFQAGQYQLLIYSGVMIALMLILPNGLLSIRPLSWRKRGRPLVWRRS"
     gene            complement(13589..14455)
                     /locus_tag="VKU03_06070"
     CDS             complement(13589..14455)
                     /locus_tag="VKU03_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012708593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70865.1"
                     /translation="MEQVVANGLYLGAQYALIALGLTLIFSLMNVLNFAHGQMYVLGG
                     FVTYEIYGQLHLPFVIALAASAVTLAILGGLIERFLFRTVIKRSVREESTMLLAAGVA
                     YFLDALILLLFGEKQRGVPKIVPGVFVSDNLIMPYDRILIAFLAVLFIVAFILMMRYT
                     KPGRAMRALAQDRVAAQLMGVPVEFYSLVGFALGALLAGLVGGLLVTITGVNSGIGGP
                     ISIKAFTMVMIGGAGVIGGAIAGGFILGMLESVGLSVLHAYGDITYLVIFAALMVFLS
                     IRPNGLMGKPWG"
     gene            complement(14524..15723)
                     /locus_tag="VKU03_06075"
     CDS             complement(14524..15723)
                     /locus_tag="VKU03_06075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ70866.1"
                     /translation="MARTALPFRLALFGFASAFAMALSAGAADAANKTLNIGFVGVTS
                     GPAAAWGTSNVRSMQTLAAWLNEKGGVKIGDDTYTINIVTFDDQKDPKRSIAGMEKMA
                     QEGIHYVVGPNVDDGAAAVRPVAEKSGIIYFPYAFPKELYTKPASNAILGMVANYQSA
                     PAIYKYLKEKKGVKTVAFVAANESDPLSQRDGGVAAAKALGLTVLADKDTYENDTRDF
                     TPVLTPIVKLKPDLLVLSGVAPANAPQLIRSARELGYKGLISTETAQDAKVLQEGAGD
                     LANGFISVGGASTPEIRSKTMEEFIQRYTKMFGEYNDESNTKVYALEYIVDTLKADPK
                     AIDNVEEFKKTIDTFSAPNPYLKNGGTLHYVGMTSFGQKRQIAVPLVVNEYKDGKFET
                     LFVADVD"
     gene            15954..16649
                     /locus_tag="VKU03_06080"
     CDS             15954..16649
                     /locus_tag="VKU03_06080"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ70867.1"
                     /translation="MDALNRDLRGPVSDDQIVDRIVAAVMERRLKPGAKLPENTLCDA
                     FGVSRSQIRRVFIVLAQRGIVALHPNRGAFVASPTPEEARYVFEARRTLERSIIAETA
                     RRITGEQIGEMRNYVAGSAAAAAMDNRGEAVRLSGEFHIQLARVAGNPVTARFVEELV
                     ARTSLILAMFGSRQSFSCSEGEHNELLKALEDHDGARATALMDHHLEHIERELDIRDA
                     EAEATDLREILSL"
     gene            16777..17757
                     /locus_tag="VKU03_06085"
     CDS             16777..17757
                     /locus_tag="VKU03_06085"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014132795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ70868.1"
                     /translation="MDYIRFGKTGMKVSRLCLGCMTYGSTKWREWVLEEEASRPFIKE
                     ALEKGINFFDTADIYSIGASEEILGRALKDFAKRDEVVVATKVFNAMGTSPNARGLSR
                     KHILEAIDASLKRLGMDYVDLYQIHRFDYETPLEETVEALSDVVRAGKALYVGASSMW
                     TWQFARMIGYAREKGLAEFVSMQNYYNLVYREEEREMMKFCADQNIAVIPWSPLARGF
                     LAGSGVGAEASTVRGRTDQYSRQLGLGARQDETIRKRVNETAEKLGAKPASVALAWVL
                     SKPYVTAPIVGASKPHHLDDAIAALSLKLDARTIAKLEEPYQAKAVVGHQ"
     gene            17754..>18499
                     /locus_tag="VKU03_06090"
     CDS             17754..>18499
                     /locus_tag="VKU03_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLJ70869.1"
                     /translation="MTRNRYYSGPISDHFDGELFFAPARPADRTLRDILAWRFHERAA
                     PWPRAYPSPFSDRPPYRVEGGALRVSYIGHASFLIQTRGLNLLIDPVFSKRVSPASWA
                     GPARVNPPGIAFDDLPPIDAVLVTHNHYDHLDLHTLSKLNARWRPRILTPLGNDAIMR
                     AHDRALRPEAHDWGEKAALSPGITAHFAPAYHWSARGLRDRRMALWCAFIIETPDGAL
                     YCIGDTGFGDGAIFRDVAEKFGPPRLALIP"
BASE COUNT         3151 a         5998 c         6090 g         3260 t
ORIGIN      
        1 ctcgtctcgc ttggcgcgtc ctgtttcgtc ggattgggcg cctatatcgc tttcggggtt
       61 ctgaacaagc tggatgcgcc gctgcctttg gcgctcgccg ccgcaggcct gggcggcggc
      121 gcactcgcgg ccctcgtgtc gccggctctg tttcggctgc gcggcctcta tttcaccgtc
      181 ggaacgctag ccctcgccga attgctgcgg ctgaccatga tcaatgtctc cgcctttggc
      241 ggcgccaccg gtattttctt gaagaacgat ccgcctgact tgccgatact ctttcgctac
      301 gcgctggctg tcgcgttgct ggcctcgctc gccatgacgt tctgcgcccg cagccgcgca
      361 tcggtgattc tgcgcgcggt gcgggacgac gaggacgccg ccgcgcagat gggcgtgagg
      421 ccgttccgcg tgaagcttgc cgccttcgtc cttgccagcg cggtcgcggc catggccggc
      481 ggcgtgcagg cggtcaagct cggcgcgatc gagccctatg gcatgttcgg cctgcaatgg
      541 tcgatcgacg tcctgaccat ggcgattatc ggcggcgtcg gactgcggct cggcccgctg
      601 gtcggcgccg tcttcgtcat cgcgcttggc gaggcgttgg ccgattatcc cgaggcgcat
      661 gtcgcgatcg ccggcgtgat cctgatcgtg ctgatccgct tcgcgccgcg cggcgtcgtc
      721 ggcttcgtcg ccaatccggc gctggttttg cgaaggccgg cgccggcatg agcgcgccgc
      781 cgcttttgga agtctctggc ctctccaagc gctatggcgg cctggtcgcg atcgaccatc
      841 tcgatctgag cgcgccggcc ggcgcaatca ccggcgtcat cggtccgaac ggcgccggca
      901 aatcgacgct gatcggcttg atcggcggcg cgttggcgcc cagcgccggg acgatccggc
      961 tcgacggcaa ggacgtgacg cgccttccgg cgtccgaacg ggcgcgcgcc gggatcgggc
     1021 gcacctatca aattccacgt ccctttctcg acatgaccgt cgaggaaaat ctcgaagtcc
     1081 cgcaatattc gatcgcgccg ttcatcggtg ccgccgcggc gcgggaggag cgcgaggcgc
     1141 tgcttgttcg cacgggcctt gccgacgccg cggccctgcc ggcccgcgct ctgccgcttc
     1201 tcaggcgcaa gcgcttggag gtcgcccgcg cgctggcgct gaagccgcgc ctgctgctgc
     1261 tcgatgaagt cggcgccggc ctggtcgaca gcgagatcga cgagctgatc gcgctgatcc
     1321 aatcgatcct cgacaaaagc acggcgatcg tcatcgtcga gcatgtcatc cggatcgtgc
     1381 gcgaatgctg cgcccgctcg ctggtgctca atttcggcaa gaggctgatc gaggggccga
     1441 catcggagat tctcgccaac gatcaagtcg ccgcggtcta tctcggcacg ggcggcgctc
     1501 accgcaaagc cgcgacgggc gcggagacgt ctcgggcgga cggcgccatt catgtcgccg
     1561 cgccggcgga ggaacggcgc aagctcgcgc cgctggtcgc ccctgccgcg gattcgaaag
     1621 agccgatcct ggagcttcgg aacgttgcgg cgggctatgg ccaggcgcgc gtgctgaaag
     1681 gcgtcagcct ggccttggcg gaaggcgagg cgatcgcgat tctcggcgcc aacggcgccg
     1741 gcaagacgac gctcgcccgc gtcatttgcg gcgccgtcgc gccgagcgcc ggcatcctgc
     1801 gcatcggcgg gcgcgccgcg gggctcgcgc cgcatcgggt cgccgctttg ggcgtcgccc
     1861 attgcatgga ggggcggcgc atcttctcca gcctgtcggt cgaggagaat ctgcgcatcg
     1921 ccgcgcgcgg cgccgacgcg gcgacggcgc gccagcggct cgaggcggtc tacgatctgt
     1981 ttcccgtgct tttcgagcgc aaagcgcagg tcggcacgtc cctctccggc ggtcagcagc
     2041 aaatgctggc catcgctcgc gctctgatgg cccggccgcg cctggttatt tttgacgaaa
     2101 tctcgctcgg gctcgccccc gtgatcatgg acaagcttta cgaggccttg gcgcagctca
     2161 agcaagcgcg catgaccctc attctcatcg aacaggacgt cgaccgcgcg ctcgctctgg
     2221 ccgaccgcgc ttatgtgctg gagcaagggg ttttcgcgct ttccggcgcg gcgcgcgaca
     2281 tcgccgccga cccgcggctg cggcgcgtct atatgggcgc cgcctgattc gcctgtcagg
     2341 ataagcggca acggctatcg agccggcggc cggctgcgct acatttccaa cgggttggag
     2401 gcggaccggg cagtttcatg gcgcgccgat atcttgatca acgcttacgg ctccatctgc
     2461 ttcgcgccgc cgacgcgatc gaggcgcagg gcagcttgat caaggcgtcg gcggcgctcg
     2521 gcgtcagcca gccggcgttg accaagagct tgcacgagct cgaggccatt cttcagcttc
     2581 gattgttcga tcgccattcc cgcggcgtga gcccgaccga agccggcgtc gccttcgtcc
     2641 agtgcgcgcg gcgcgtgttg gccgagttgc accggctcga cgagcaactc gatcggcttt
     2701 ccagcctgga gggcgggacg gtggcgatcg gcgccctgcc ggtcgccgcg gccgggctgt
     2761 tgccgggcgt gctgacccgg ctcagggctg cgcatccggc ggtcagggtc cggttgctgc
     2821 aggggcgaac gcgtgaattg ctgccgctgc tcgcctccgg cgagatcgac ctcatcgtcg
     2881 ggcggctcta tgaacccgcc gttccggacg gctttatccg cgaggcctta tggaccgagc
     2941 cgatctccat tctcgctcgc gccgggcacc cgattttttc cgaacctgtc actgtcgagg
     3001 cgttacggcg ctacgatttg gttctcccga cgatcaccca gcgcgtcggc gaggaggtcg
     3061 aaggcctcat tgcgcagctc ggcctcgacg ccgcggcgtc gctgcgctcg agctcgtaca
     3121 gcttcatccg cgagatgttg cacggaaccg acctgctcgc catcgtgccg cggctcatga
     3181 tggtcggcga tctgttgcgc ggcgatttgc gggtagcgcc gctgccgctc gccgcgccgg
     3241 accggccggc cggacttatt ctgccgcgcg gccgcccgct gcccgcggcc gccgagttct
     3301 tggcgacgtc gctccgcgcc tatgtcgcgg aaatcgccca actcggtctt gccgcgccta
     3361 taactgcgag tgatggcgcc ggcgcgagaa gcgatgggac gcgtgatgca acgccggcat
     3421 aaatcgcggc tgcgcgggag gcgtcgaaca gggagaatcg caccgtgagc gaatctgcaa
     3481 ataagacggc gctggtcgtc agcgcccatt cggcggactt cgtctggcgc gccggcggcg
     3541 cgatcgcgct ccatgcggaa cgcggttttg cggtgacggt gatctgcctc tcctatggcg
     3601 agcgcggcga atcggccaag ctctggcgcc agcaaggcat gacgctcgat cgggttaaaa
     3661 cagagcggcg aaaggaggcc gaggccgccg cggccaagct cggcgccgcc gacctgcaat
     3721 tttgggatct cggcgattac cccatcacgc tatcggaaga atcgctctgc cggctcgtcg
     3781 atgtctatcg cgccatccat ccgcttttcg tgctctcgca ttcgcgccgg gacccgtaca
     3841 atcgcgacca tccggccgtc agcgactttg ctcagcacgc ccgcatcgtc gcccaggcgc
     3901 atggccataa gccgggcgag cgcgtgcttg gcgccccgcc ggtctttctg ttcgagccgc
     3961 accagccgga gcaatgcggc tggaagccgg acgtgctgct cgacatcacc tcggtatggg
     4021 agaagaagcg cgccgcgatc gaatgcatgg cgggccagga gcatctctgg gattattaca
     4081 cgcgcgtcgc ccagcagcgc ggcgtccaag cagcgcgcaa ttcgaataag aagatcgctt
     4141 atggcgaggg atacgaagct gtgttcccgc atgtcgtgga gagcctggcg tgagcgccgg
     4201 cttcgtcttc accgatccgc agcgggtcgc cccggagctt gcggcggcgt tcgcgcccta
     4261 cggcgtcgcc acgattcacg aggcgcaggg ccgcgccggg ctgctcgcct catatatgcg
     4321 gccgatctat ccgggcgcga ggcttgccgg cagcgccgta acggtctcgg ttccgccggc
     4381 cgacaattgg atgctgcatg tcgcagtcga gcaatgccgc gccggcgacg tgctggtcgt
     4441 cgccccgacc tcgccttgcg acgccggcta tttcggcgag ttgctggcct cctcgcttgt
     4501 cgcgcgcggc gtcgtcggcc tgatcatcga agccggcgtg cgcgacgtgg cggcgctgac
     4561 caaaatgcgc ttcccagtct ggtccaaggc ggtctcggcg cagggcacgg tcaaggagac
     4621 gctgggctcg atcaacgcgc cgctggtttg cgccgggcaa ctcgtccatc ccggcgacct
     4681 gatcgtcgcg gacgacgacg gggtcgtcgt ggtgccccgc ctcaatgccg aggacgcgct
     4741 gcgcaagagc gaggcgcgcg agcgcaagga ggcggcgacg cgcgcccggc tgcaagccgg
     4801 cgagctcggc ttggatattt acgccatgcg cgagaagctt aaggaaaagg gtttggtcta
     4861 tcgtcccttc gcggatcggg agggctgaaa tggcgcccgg cgcgaccaac gccgaggccg
     4921 gcgcgcctgc gctccgcgcc gcgatcggcg cctacaagca cactcaggcg ctcaaatccg
     4981 gcgcggtttc atcgagcaag ctccgcctgg atttcgctga cgtctcgccg atcagccgcg
     5041 cgttcgcgcc gatggttcga gagttgcgct tcgatgtttg cgagatggcg atcgcgacgt
     5101 ttctccaagc caaggcttat ggcaagccgc tcgtcctgct cccgatcgtc gcggccgcgc
     5161 gttttcagga gtcggctttg ctctgccgcg ccgacagcgc gattcgcggc cccgccgatc
     5221 tcgccggccg ccgcatcggc gtgcgcgcct acagccagac gacgggcatg tggctgcgcg
     5281 ggattctcgc agaagacttc ggcgttcggc cggaacaggc gcgctggatc acgttcgagg
     5341 atgcgcatgt cgccgagtat cgcgatccgc cttgggccga gcgcgcgccg ccaagcaagg
     5401 cgctcctcgc catgctgcat gcgggcgaga tcgatgcggc gatcctcggc aacgacgtgc
     5461 cggacgaccc tggttttcgt accgtcttcc cggatccctt cgcggccggc gaggctttcc
     5521 gccgccggcg cggcttcgtc ccggtcaatc atctggtgac gatgcggcgg gatctggcgg
     5581 acgcccgacc cgacttggcc gccgagatcg tcggcctatt caaggaagcg aaagccacgg
     5641 ccaatctttc gcccgctcag caggacgccc tgccgacgac ccgcgccgag ttgcgcgcag
     5701 ccgtcgctct tgcttcgcgc tattcggccg atcaaggctt gctgccgcga cgcctctccg
     5761 aagacgaagc atgggagggt tcgcccgatc ttgatggccg agcaagcagg ctgtagccgg
     5821 agcatccttt tgcttcgctg agcgaaagct ttcgctcagc ctgtatcttc cgctcttgtt
     5881 tgcgtcgccc gggcgaggaa ttcgtgcttt gcggtttcgt gctccatccg tactttgggc
     5941 gaggcgtcga gaaccaagaa tggccgttct ccgtcgcggt aggaaggcca tttcggcagg
     6001 cccgctccgt tcgggtcgcc ggacttgatg aaattcaccc aataggcgga ggcgagcgtc
     6061 gccagggcgc gatcgtcggc cgcgatccgc cacggacagg cgtcgagatt gtcgaaaacg
     6121 tagaaaatct cgccggcgtg gaaggcgccg gcttcggcgc taggcgtcga tccgaaccaa
     6181 ccttccggcg tcagcgggca acgatcgaat cggaaccgat aaatcggcgc cgttccactc
     6241 gtccgttgcg cctccagcca ggcccatgtt ccgtggacga tcgagagatc gccgccgagc
     6301 gccttcgccg aagctttgtc ggtctccgcg tcgccggatg gataaaagcg cagggcctcg
     6361 gccgtctggc cgccgaatat tccgcgaacg agcgccgggt aggattgatc gtccggcgcg
     6421 ccgtggagga gcgagaagtt gaatccttcg tcgcgattcc agcccgccag cagcggggcg
     6481 tcgctttgct cgccggcggc gaaaatctgc gccggcggcc gcggcaggaa atagccgtcg
     6541 atgatgggtc gaaagcccaa gcccggcgcg gcggcgagga tgtcctccgc cggcgcggcg
     6601 cgcagcgcct cgagcgaagc cgctcccagc ttccgggcga acgcgcgtcc cgcttcttcg
     6661 gcttcggcca gggagaggat cggatggccc ggcgaagcga acatcccgcc gctttcgccg
     6721 atcgcgcggg cgaagaggcc tttggccaag ggcgacgcca tcaagccgct gaccgccatc
     6781 gagcccgccg attctccggc gatcgtcaca cgtttcgggt cgccgccgaa cgccgcgatg
     6841 ttgcgcttca cccagccaag ggcggcgacg aggtcgagca tgccgtaatt gcccgaaccg
     6901 cgatgcggcg attcggcgga caagtcggga tgcgccagaa agcccagcgc gttcagccga
     6961 tggttcaacg tgacgacgac gatcccccgc gccgccagat tcgacccgtc gtagcgcggc
     7021 tcgctgcccg agccgacgac gaagccgccg ccgtgaatcc agaccatgac cggcaggaga
     7081 tcgccgctcc cgaccgcgcc gctccagaca ttgaggtaga ggcaatcctc cgaaacgccg
     7141 gcgggacgcg gatcgatatc gtcgaagacg attccttgaa gcgagttcgg gccgaacctg
     7201 tcggtccggc gaattccccg ccacgccgta accggggccg gcggccgcca gcgcagcggg
     7261 ccgaccggcg gagcggcata aggcacgcct ttgaagcagc gcacgcccgc cgcgttgggc
     7321 tcaggcagcg ccaggagccc atcggtggta gagactgttc cgggggtcat tgtcgctccg
     7381 tcgcccatct taacgatgat ctctatatca gcaatccggc cgagcgccgc tatcgcgatt
     7441 tcgtttttcg ccggcgcggc cgccgccgct ggctagtacg atcgcggcaa gccgagcaca
     7501 tgctcggcca agtaggacag gattaggttc gtcgaaatgg gcgcgacctg gtagaggcgc
     7561 gtctcgcgaa atttgcgctc gatgtcgtat tcctcagcga agccgaagcc gccatgggtc
     7621 tgaacgcaga cattggccgc ctcgaacgag gcgtcggcgg ccagcatctt cgccatattg
     7681 gcctcggcgc ccggatttgc gccggcctca tagagccgca gcgcctcgcg cgtcatcagc
     7741 tccgccgcgc gcatattcgc gtaggctttg gcgatcggga actgcacgcc ctggttctgg
     7801 ccgatgggcc ggtcgaagac gcggcgctct ttcgcatagg cgcttgcctt gtcgatgaac
     7861 catttggcgt cgccgacgca ttcggcggcg atcaggatgc gctcggcgtt caagccggag
     7921 aggatgtagc gaaagccctt gccttcctcg ccgatcagat tctcggccgg cgccttgaga
     7981 tcgtcaaaaa agacttcggt ggtcgcatgg ttcatcatcg tgcggatcgg tcggatcgac
     8041 aggccggcgc cgagcgcggc gcgcatgtcg acgatgagaa ccgaaaggcc gtcgctgcgc
     8101 ttggccccgg ccgattgcgg cgcggtgcgc gccaggagaa gcatcaggtc ggaatgctcg
     8161 gcccggcttg tccagatttt ctggccgttg acgatgaaat gatcgccctc gcgtcgcgcc
     8221 gtcgtcttca acgaggacgt gtccgtcccg ctggtcggtt cggtgacgcc gaaggcctgg
     8281 aggcgcagct cgccttcggc gatcgccggg agatagcgac gcttctggtc ctcggagcca
     8341 tggcgcagca aagcgcccat cgtgtacatc tgcgcgtggc aggcgccgcc attgcagccg
     8401 gcgcgctgaa tttcctccaa gaccgcggcg gcggccgaaa gcggcaggcc cgcgccgcca
     8461 tactcctcgg gtatcaggat ggacagatag cccgcctcgg tcagggcgtt cacgaattcg
     8521 gtcggatagg ccatccggcg atcgagatcg cgccaatatt cgccgggaaa tcgcgcgcaa
     8581 agcttggcga cggcctggcg tatttcggca tagggttcgt ccattggcgc catgatcgca
     8641 aatacccgat cggacccgcg cgcggcgcgc cgccgacaat tgccgccgct ataatgcgcg
     8701 gctcgccgct cggagcaact ggcgaacggc cagcttgcgg cgcgttggaa agattttggg
     8761 aggaaggccg agcggcccgc gcgcttgctt gacccggcta ttggcgcaag attgatcttt
     8821 tttgggatcg ccgcgatgga cctgcgatcg tttcttggag agccgacagg ccaaacggcg
     8881 cttcagcttg ccgaactcgg cttggcgttc attctgtccg cgctgatcgg gctggagcgg
     8941 gaaatccgcc agaagagcgc cggcctgcgc acctatacga tcgtcggctt cgcctcggcc
     9001 ttgatcatcg ttgtctcaaa atatggcttc accgacgttc tcgaacagaa tcgtatcgtt
     9061 ctcgacccgt cccgaatcgc cgcgcagatc gtctcgggca tcggctttat cggcggcggc
     9121 gtcattttcg tgcgcaagga cctcgtccgc gggctgacca cggcggcgac catttggctg
     9181 accgcggcgg tcggcatggc ttgcggcgcc ggcctgccga tcctcgcgat agcagtcacc
     9241 ggcgggcact tcgtggtcat cggcctgttt cccgccgtcg agcgccgcct tcccaaatcg
     9301 cgctgggcgc cgtcccagct ccacatctcc tatcgtggcg gccagggcgt tctaagggaa
     9361 gtcctcgcca tctgcaccca gcacgatttc gcggtgagcc gcgtccaggt cgagggcgac
     9421 ccgtcgcctc gccgcaagca atcgggagga cagcaggcga aaggtcacga tatgcagtgg
     9481 gacgacgaag cgtcgagctt cgaggagacg ccggcaagcg aagcaggcag cgacggaaat
     9541 ggaatcgtga cgcttctcgt cgaacttcgc ggcaccaagt ccattccgaa gctcgcggcg
     9601 cggatcagcg aaattcccgg ggtcgtttcg gtccaggccg gcgacgcaaa cttcctctcg
     9661 gagtgacgcc cgtcaaacct tgaaattccg tatcagcgtc acgaggcctg gctcgtcgac
     9721 aagcgtcgcc gcatcctcct gcgggagcca gccgattcgt cgggcgttct tttccggcca
     9781 atcagcgcgt tgcgtctcga cctccaaagg gtagacgtcg acggcgagca attcaaagct
     9841 gcgctcgagg cgcttccagt agcgatattc gccgatcggc ttccgcctca ccttgccaac
     9901 caccccggcc tcttcatagg cctcgcgggc ggcggcgtcg gaatcgctca gccgcttcat
     9961 cggccagccc ttcggaataa taaaccgccc ggtttcgcgc gatgtgatca gcaggatttc
    10021 gatcccgccg gatgcggtct tgcgaaaggg cagcgccgcc acttgccgcc tttcggcggc
    10081 ttgcggcgcc gagccgggct catcgcgacc gcgaggcttg gggcgcgggc ttatagtgcg
    10141 tttcatcgcc ttcatcgcaa cgcagttctc ggccccgaat cgatgcaagc gtcatgctag
    10201 gcttcatccc ttattgaaac gtcaaaagct tggcgggtcc agccggtccg acgagctact
    10261 ccgaccgccg cgcgaagccg agcgggcgat tgcgatcacc cgcaggcgat caccgcatct
    10321 tcgccgcgct ggcgctcggc gagaagctcg ccaagagccc ggcataggct ttcggcagcg
    10381 gcgcggcata ggtgttgagc ttggacgtgc gcagcccgat ccgggcggcg gcttcggcga
    10441 gcgcgacggc gcaagcgacg ccgtcgagca ccggcgcgcc cgtctcttcg gaaagatcgc
    10501 gggcgagctc cgtcatgccg gcgcatccga gcacgatcgc ctcggccccg tcctcgacga
    10561 gcgatttgcg cgcctcatcc agaatgaggc ggcgcgcatt ggagcgcgcg tcctcgaggt
    10621 cgagaacggc gacctggcag gcgcggacgc gggcgcaacg gctggccagc ccgtatttgg
    10681 ccaggttctt ctcgatcggc gcgatcgagc gcggcaaggt cgtcaccacg ccgaaccggt
    10741 ccgcgatcag gctcgccatg agaaagccgg cctcgccaat gccgacaacc ggcgctttgc
    10801 tgacgcagcg cgccgcatcc aggccggtat cgtcgaaaca ggcgatcacc gtcgcgtcga
    10861 cgtcgcccgc ctggcgcatc tcttcgatga cgcctgggat cgagaagacc tcgtcgtaat
    10921 agccttcgat gctcggcggc ccggaatccg ggttgaccgc gaggatctcg acgccgggcg
    10981 aggccgccgc ccgcgccgcc gcgccgatct tccgcgtcat cgcggcggtg gtgttcggat
    11041 tgacaacaag gatgcgcatg gaaggccggg cctctaaaga tcgccgccga gatagagctg
    11101 ttggacgcgc gcgtccttga cgagatcggc ggatgcgccg gtgagcgcca ccgatccggt
    11161 cgtcaaagcg taggcccgat gggagacgcg cagggccatg cgcgaattct gctcgaccag
    11221 caaaacgctg accttgtcgt cgcgattgat cgaaacgatc gagcgcgcga tgtcctgcac
    11281 gattttcggc gcgacgccga gcgacggctc gtcgagcagg agcagcctgg gcttggccat
    11341 cagcgcgcgg ccgatcacca gcatctgctg ctcgccgccg ctcatcgtgc cggccgcctg
    11401 gcggtagcgc tcctgcaagc gcggaaagcg cgccagcacc atgtccatcg tggcgcgcac
    11461 gccggccgaa tccttgcgcg agaacgcgcc catcagcagg ttgtcgcgca ccgacatata
    11521 ggggaagacg cggcggcctt ccggaaccat ggcgatgccg aggccgacga tcgcctccgg
    11581 cccgaggccg tcgatgcgtc ggccctcgaa atggatttgc ccgctgcgaa tttttcgcaa
    11641 gccggtaatc gcccgcagga tcgaggactt gccggcgccg ttggcgccga tcaaggccac
    11701 ggtttcgcct tccatcagtt ggagcgacac gcctttcagc gcgtagatgt ggtcgtaaaa
    11761 gagctcgaca ttggcgaagg tcagcatcgg ctgcatggtt taaacgccga tcgtctcgtc
    11821 ctcgacgccg agataggcct cgatgacttt ttcgttcttc tggatttccg ccggcgtccc
    11881 ttccgcgatt ttcgcgccga aattgatgac gacgatgcga tcggagattt tcatcacggc
    11941 ggccatatcg tgctccacca gcaagagcgt gacgccgcga tcgcggacgg cgcgggtcat
    12001 ttcgacggcg cgccgcgtct cgtcgtgatt cattccggcg aaaggctcgt cgaggagcag
    12061 aatcgtcggg ttggcggcga gcccgatcgc cacgccgagc gcccgcaggc tgccttgcgg
    12121 caggttgctg gcgatctcat tggcggcggc gcgcagcccg agaaagtcca gtatctcgtc
    12181 ggccgagcgg ccgaactccg cttcgtcgcg gcgagccgcc gccgtgccga gataaaagcc
    12241 cgcgaggccg gctttcgatc gcaaatggtg agcaataatg acgctgtcgc gcgccgtcat
    12301 gcctttgaag atcgtcgtct cctgaaaggt gcgaacgacg cccttgcgcg cgaccgtatg
    12361 gggcgcgagg ccggaaatgc gctcgccgcg gaaacgaact tcgccgcgcg tcggcttgac
    12421 gaacgacgcg atcagcttga acagggtcga ttttccggcg ccgttggggc cgatcaccga
    12481 gaggatctcg ccttgctcga cctcgaacga cacgtcgttg acggcgacga ggccgccata
    12541 ttgcttggtg atgccggaga tcgcgaggat cggcgccatg ctcagctcct gcgccagacc
    12601 agcggtcgcc cgcgcttgcg ccagctcagc ggacggatgc tgagcagccc gttcggtagg
    12661 atgagcatga gcgcgatcat cacgcccgaa tagatgagga gctggtactg cccggcttgg
    12721 aagagcagat cccagccgaa atagaggacc agcgttccga gcatcggccc gaacacgtag
    12781 ccaaggccgc cgaggaagca attgagcatg aaattgatgc tgtcggcgac ggtgaagctc
    12841 gatggataga tcgattgcgc aatggcgacg aagatcgccc cgcctatgcc gccgaaaaag
    12901 gacgagatcg aataggcgag cacccgcaaa gccggcacgt tgaggcccat cgacgaagcc
    12961 aattcttcgt tctgctgcaa ggcgacgaac aggcggccga cgcgcgaatt caccagtcga
    13021 taaagcgccg cgaagcagag acacatgatt gtgacggaga ggtaatagaa cgcctcattc
    13081 cgctcgacga agacatcgaa ggacggaata agggtcacgc cgaacaaggc gatcgggccg
    13141 ggcggcggaa tgctgaccat gcccttggcg ccgttggtga tcgacaaggc catggcgagc
    13201 aggcgcacca cttcggtcaa aaccagagtg accatagcga aatagacgcc gcgaaggcga
    13261 aggatcggca ggccgatcag aacgctcgcc gccgcgcaga acgcgccggc gaccggcagg
    13321 ctgacccaga acgagaggcc gcagcggacg atcaggatcg ccgagacata gccgcccatc
    13381 agggcgtagg cgccctggcc gatattgatg cgcccgatat agaaggtgag ccaaacgccg
    13441 ctgctggcga tcgcgaggag cgcgaccgag ctcagcgtgt agtagaagtc cgtccgatgg
    13501 gtgaggccga tggcggccgg gaccgcgacg aagacggcca acagaaagac ggcgagcgcg
    13561 acgagctggg cggggcgcga catcaggatc atccccaagg cttgcccatc agcccattcg
    13621 ggcggatgct gagaaacacc attaaagcgg cgaagatgac gagataggta atgtcgccgt
    13681 aagcgtgcag cacgctcaag ccgaccgatt cgagcatgcc gaggatgaag ccgccggcga
    13741 tcgcgccgcc gatcacgccc gcgccgccga tcatcaccat cgtgaaggcc ttgatcgaga
    13801 tcgggccgcc aatcccggaa ttgacgccgg taatcgtgac gagcaggccg ccgacaaggc
    13861 cggcgagcag cgcgccgagc gcgaagccga cgagggagta aaattcgacc ggcaccccca
    13921 tgagctgcgc ggcgacgcgg tcttgcgcca gcgcgcgcat ggcgcggccc ggcttggtgt
    13981 agcgcatcat cagaatgaag gcgacgatga acagaacggc gaggaacgcg atcaagatgc
    14041 gatcataggg catgatcaga ttgtcggaga cgaagacgcc cggaacgatt ttggggacgc
    14101 cgcgctgctt ctcgccgaac aggagcagga tcagggcgtc gaggaaatag gcgacgccag
    14161 cggccagcaa catcgtgctc tcttcgcgca cgctgcgctt gatcaccgtg cgaaacagaa
    14221 accgctcgat gaggccgcca aggatcgcca atgtcacggc ggacgccgcg agcgcgatga
    14281 cgaaaggtag atgcaactgc ccgtaaatct cgtaggtgac gaagccgcca agaacgtaca
    14341 tctgaccgtg cgcgaaattg agcacgttca tcagcgagaa aatgagggtc aagccgaggg
    14401 cgatcagcgc atattgcgcg ccgagataga gaccgtttgc gacgacctgc tccatggccg
    14461 agcgtcccct ttaaggagag gcgggagcgc cgcctctcct tgccaagcga atgggcctta
    14521 atgtcagtcg acgtcggcga cgaacagcgt ctcgaacttt ccgtccttgt attcgttgac
    14581 gaccagcggc acggcgatct gccgcttctg gccgaacgag gtcatgccga cataatgcaa
    14641 cgtgccgccg ttcttgagat aggggttcgg cgccgagaac gtgtcgatcg tcttcttgaa
    14701 ctcctcgaca ttgtcgatcg ccttgggatc ggccttcagc gtgtcgacaa tgtattcgag
    14761 cgcgtagacc ttggtgttcg attcatcgtt atactcgccg aacatcttag tgtagcgctg
    14821 gatgaactcc tccattgtct tcgaccggat ttccggcgtc gaggcgccgc cgaccgagat
    14881 aaagccattg gcgagatcgc cggcgccttc ctggagaacc ttggcgtctt gcgccgtttc
    14941 ggtcgagatg agtcctttat agccgagctc gcgcgccgac cggatcagct gcggcgcgtt
    15001 ggccggcgcg acgccggaga gaacgagcaa atcgggcttg agcttgacga tgggcgtcaa
    15061 gacgggcgtg aagtcgcgcg tatcgttctc gtacgtatcc ttgtcggcga gcacggtgag
    15121 gcccagcgcc ttggcggcgg cgacgccgcc gtcgcgctgg ctgagcggat cggattcgtt
    15181 cgcggcgacg aaggcgaccg tcttcacgcc tttcttttcc ttgagatatt tatagatcgc
    15241 cggcgccgac tggtagttgg cgaccatgcc gagaatcgcg ttcgaagcgg gctttgtgta
    15301 gagctctttc gggaacgcgt agggaaaata gatgatcccg gacttttcgg cgacgggccg
    15361 caccgccgcc gcgccgtcat cgacattcgg gccgacgaca tagtggattc cctcctgcgc
    15421 catcttctcc atgccggcga tggaccgctt gggatctttc tggtcgtcga aggtgacgat
    15481 gttgatcgtg taggtgtcgt cgccgatctt cacgccgccc ttctcgttca gccaggcggc
    15541 gagggtctgc atcgagcgga cgttggaggt gccccaggcg gcggcggggc cgctcgtgac
    15601 gccgacaaag ccgatgttga gggtcttgtt cgccgcgtcg gcggcgcccg cgctcaacgc
    15661 catcgcgaaa gcggacgcaa agccgaagag agccaagcga aaaggcagag cagttcgagc
    15721 catggatcgt tcctcccgtt gaggcctcac gcgatcgcct gccgctggaa ggagcaaaca
    15781 agcgtcaaga tcggctccat gcgatgatgc gcaattgtcg aagatattgt caacaataat
    15841 ttcatttttg tcgacaaaat ctctccgttc gcgtatttgt agccccattc tcttgacggt
    15901 tcggcgaatc cattggctgc gtaccagcgt gaagaggagg tggagcagaa gcgatggacg
    15961 ccctcaatcg ggacctccgc ggtccggttt ccgacgatca gatcgtcgac cggattgtcg
    16021 cggcggtcat ggagcgtcgg ctcaagccgg gcgcgaaatt gccggaaaac acgctttgcg
    16081 acgcgttcgg ggtcagccgc agccagattc ggcgcgtttt catcgtgctc gctcagcgcg
    16141 gcatcgtcgc cctgcatccg aatcgcggcg cctttgtcgc cagtcctacg ccggaagaag
    16201 cgcgctacgt ctttgaggcg cggcgcaccc tcgagcggtc gatcatcgcc gagacggcgc
    16261 gccgcatcac cggcgagcag atcggcgaga tgcgcaatta cgtcgccggc agcgctgcgg
    16321 cggcggcgat ggacaatcgc ggcgaggcgg ttcgcctttc aggcgagttt catatccagc
    16381 tcgccagggt cgcgggcaat ccggtcaccg cgcgcttcgt cgaggagctc gtcgcgcgga
    16441 cctctctcat cctcgcgatg ttcggttcga ggcagtcctt ctcctgctcg gagggggagc
    16501 acaacgaatt gctgaaagcg ctggaggacc acgacggcgc gcgcgccacg gcgctgatgg
    16561 accatcacct cgaacatatc gagcgcgagc tcgacattcg agacgccgaa gcggaagcaa
    16621 ccgatctccg ggaaatcttg tctttgtgac gccgccgccg gcaagcctcg cattcgtgaa
    16681 agcgcgttct cgcttttggc gattgacatt cctcgccaat cgcgcagctt tcaccgcgcc
    16741 gcttgcgcgg cgtccgctga acggggcgaa gaagaaatgg actatatccg ctttggcaag
    16801 accggcatga aagtctcgag gctgtgcctc ggctgcatga cctacggctc gacgaaatgg
    16861 cgcgaatggg tgctcgaaga ggaagcctcg cggccgttca tcaaggaggc gctcgaaaag
    16921 ggaatcaatt tcttcgacac ggcggacatc tattcgatcg gcgcgagcga ggagatcctc
    16981 ggccgcgctt tgaaggattt cgccaagcgc gacgaggtgg tcgtcgcgac gaaagtcttc
    17041 aacgcgatgg ggacgagccc gaacgcgcgc ggcctgtcgc gcaagcacat cttagaggcg
    17101 atcgacgctt cgctgaagcg gctcggcatg gattatgtcg atctctacca aatccatcgc
    17161 ttcgattatg agacgccgct cgaagagacg gtcgaggcgc tgagcgacgt cgtgcgggcg
    17221 ggcaaggcgc tctatgtcgg cgcgtcctcg atgtggacct ggcaattcgc ccggatgatc
    17281 ggctacgccc gcgagaaagg cctcgccgaa ttcgtctcga tgcagaatta ctacaatctc
    17341 gtctatcgcg aggaagagcg cgagatgatg aaattctgcg ccgaccaaaa catcgcggtc
    17401 atcccgtggt cgccgctggc gcgcggcttt ctcgccggct cgggcgtcgg cgcggaggcc
    17461 tcgaccgtgc gcgggcgcac cgatcaatat agccggcagc tcggcctcgg cgcgcggcag
    17521 gacgaaacga tccgcaagcg cgtcaacgag accgccgaga agctcggcgc gaagccggcc
    17581 tccgtcgcgc tcgcctgggt cctatcgaaa ccctacgtca ccgcgccgat cgtcggcgcg
    17641 tcgaagccgc atcatctcga cgacgcgatc gcggctctgt cgctgaagct cgacgcgcgc
    17701 acgatcgcca aactcgaaga gccttaccag gccaaggcgg tcgtcggtca tcaatgacgc
    17761 gcaaccgcta ctattcgggt ccgatcagcg atcatttcga tggagaattg ttcttcgcgc
    17821 cggcgcggcc ggccgaccga acgctgcgcg acattctcgc ttggcgcttt catgagcggg
    17881 cggcgccctg gccgagagcc tatccgagcc cattttcgga tcggccgccc taccgggtgg
    17941 agggcggcgc cttgcgcgtt tcctatatcg gccacgccag tttcctcatc cagacgcgcg
    18001 gcctcaatct gctcatcgat ccggttttct ccaagcgcgt cagcccggct tcctgggcgg
    18061 ggccggcgcg ggtcaatccg ccgggaatcg ccttcgacga tctgccgccg atcgacgccg
    18121 tcctggtgac ccacaaccat tacgaccatc tcgacctcca tacgctttca aagctcaacg
    18181 cgcgctggcg gccgcgcatc ctgacgccgc tgggcaacga cgcgatcatg cgcgcgcatg
    18241 atcgcgcgct tcggcccgag gcgcatgatt ggggcgagaa agccgcgctg tcgccgggca
    18301 tcaccgcgca tttcgcgccg gcctatcatt ggtcggcgcg cggtttgcgc gaccggcgca
    18361 tggcgctgtg gtgcgctttt atcatcgaga cgccggacgg cgcgctttat tgcatcggcg
    18421 ataccggctt cggcgatggc gcgatcttcc gcgacgtggc ggaaaaattc gggccgccgc
    18481 ggctggcgct gatcccgat
//
LOCUS       DATGJW010000197         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_969279, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000197 DATGJW010000000
VERSION     DATGJW010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_969279"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1204
                     /locus_tag="VKU03_06095"
     CDS             65..1204
                     /locus_tag="VKU03_06095"
                     /inference="COORDINATES: protein motif:HMM:NF012377.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cellulase family glycosylhydrolase"
                     /protein_id="HLJ70870.1"
                     /translation="MTAPLSRRAALAAFAAALLAPRESAAAVAEDRVKRLARGFNLPD
                     QAPLAPGKRPDRATLAWLRRRGMSHVRLPFLGEAAMARFAERDRIKTALDDLDRALDL
                     LLDLDFAVSVDMHPGGDFQRLHRAEPDVAYDALAEAWRIIAARIARRPPERVFAELLN
                     EPNVDDAIWREQAARLAAALRADLPATTLIAGPAPYQRVEALAAWRPLDDGNVVYAFH
                     YYDPMLFTHQGMTWDPSDPLSRLAGVPYPARRGDPSIARLVETLKERGDTLLVDAFDR
                     ALARPWTKETIEAQFAPLAAWSRAHSAPVILNEFGVLRFKAPRAARLEWLRDVREAAE
                     ASGFGWAHWDYREGFGLLDEQGRPDRELIDALLPPRRAAAAGSRP"
     gene            1201..>2219
                     /locus_tag="VKU03_06100"
     CDS             1201..>2219
                     /locus_tag="VKU03_06100"
                     /inference="COORDINATES: protein motif:HMM:NF014051.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLJ70871.1"
                     /translation="MKLVARFARFAMMQRAMVGATLTLGIKASGALLMIAVFTLAARS
                     MSTAKFGEIAMWFNALSCLAVASTFGQDTLIVRSWGEYGEQSRPDLMVGAYRFGWATV
                     AICAGLACAALLVADRLISAPLSGFALAAACAFLAAQIFQHYSSHSSRTIRGYVVSEP
                     HRELTWRLILLAVVALHLNGALTLETFFFAAAAGMALSTAIQTFAVLRKFPVQQRPAY
                     LTREWLGRALGMCLSASVEALSQYAEVIILGLLATPSAAAGYFIAMRIANIFPMMSTG
                     LNTYTVTKASSLHFGGQTARLQGVMRSVMTVAALIATPTFIALTLVAQPILSIFGPSY
                     VADYPA"
BASE COUNT          293 a          783 c          728 g          415 t
ORIGIN      
        1 ggctgtttct tcgctatctg atcaccaatc cgcatgcgct cttcgtgttg gcgcgggacc
       61 tgaaatgacc gcgccgctca gccgccgcgc cgccttggcc gcgttcgccg ccgcgcttct
      121 cgcgccgcgc gagagcgccg ccgcggtcgc cgaagatcgc gtcaagcggc tggcgcgcgg
      181 ctttaatctg cccgatcagg cgccgctcgc tcccggcaaa aggccggatc gcgcgacgct
      241 cgcctggctg cgtcgccgcg gcatgagcca tgtgcgcctg ccctttctcg gcgaggcggc
      301 gatggcgcgc tttgcggagc gcgatcgcat caagacggcg ctcgacgatt tggaccgcgc
      361 gctggattta ttgctggatc tcgatttcgc ggtcagcgtc gacatgcatc cgggcggcga
      421 tttccagcgc ctgcatcgcg ccgagccgga tgtggcgtac gacgctttgg cggaggcgtg
      481 gcggatcatc gcggcgagaa tcgcccggcg gccgccggag cgggtcttcg ccgaactcct
      541 caacgagccg aatgtcgacg acgcgatctg gcgcgagcaa gcggcccgtc tcgccgcggc
      601 gctgcgcgcc gatctgccgg cgacgaccct cattgccggc ccggcccctt atcagcgcgt
      661 cgaggcgctc gcggcctggc ggccgctcga cgacggcaat gtcgtctacg ctttccacta
      721 ttacgacccg atgctgttca ctcatcaggg gatgacctgg gatccttccg atccgctgtc
      781 gcgccttgcc ggcgtcccgt accccgcgcg ccgtggcgat ccgtcgatcg cgcggctcgt
      841 cgagacgctg aaagagcgcg gcgacacgct tctcgtcgac gctttcgatc gcgcgctggc
      901 gcggccttgg accaaggaga cgatcgaagc gcaattcgcg ccgctcgccg cctggagccg
      961 cgcccattcg gcgccggtca ttctcaacga attcggcgtg ctgcgcttca aggcgccgag
     1021 ggccgcccgg ctcgaatggc tgcgcgacgt gcgcgaagcg gccgaggcga gcggcttcgg
     1081 ctgggcgcat tgggactatc gcgaaggctt cggcctgctc gacgagcagg gccggcccga
     1141 tcgtgaactg atcgacgcgc tcctgccgcc gcggcgcgcg gcggcggccg gatcgcgtcc
     1201 atgaagctgg tcgcgcggtt tgctcgtttc gccatgatgc agcgcgccat ggtcggcgcg
     1261 acgctgacgc tcggcatcaa ggcgagcggc gcgctgctga tgatcgcggt cttcaccctg
     1321 gcggcgcgct cgatgtcgac cgccaagttc ggcgagatcg cgatgtggtt caacgcgctg
     1381 tcctgcctcg ccgtagcctc gacgttcggc caggacacat tgatcgtacg ctcctggggc
     1441 gaatatggcg aacagagccg gcccgatctg atggtcggcg cctatcggtt cggctgggcg
     1501 accgtcgcga tctgcgccgg cctcgcctgc gccgcgctgc tcgtcgccga tcggctgatc
     1561 agcgcgccgc tttccggctt cgcgctcgcc gcggcctgcg cctttctcgc cgcgcagatc
     1621 ttccagcact attcctcgca ttcctcgcgc acgatccgcg gctatgtcgt ctccgagccg
     1681 caccgcgaat tgacttggcg gctgatcctc ctcgccgttg tcgcgcttca tctgaacggc
     1741 gcgctgaccc ttgagacctt cttcttcgcc gccgcggcgg ggatggcgct ttcgacggcg
     1801 atccaaacct tcgcggtttt gcgcaaattc ccggtccaac agcggccggc ctatctgacg
     1861 cgcgaatggc tcggccgagc cttgggcatg tgcctttcgg ccagcgtcga ggcgttgagc
     1921 caatacgccg aagtgatcat tctcggcctg ctggcgacgc cttcggccgc ggccggctat
     1981 ttcatcgcca tgcgcatcgc caatattttc ccgatgatgt cgaccgggct gaacacctac
     2041 accgtcacca aggcgagcag cctgcatttc ggcggccaga cggcgcggct gcaaggcgtg
     2101 atgcgctcgg tgatgaccgt ggcggcgctg atcgcgacgc cgaccttcat cgccctgacg
     2161 ctggtcgcgc agccgatcct gtcgattttc ggcccgtcct atgtcgccga ctatccggc
//
LOCUS       DATGJW010000198         2965 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_243587, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000198 DATGJW010000000
VERSION     DATGJW010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2965)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2965)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2965
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_243587"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..134
                     /locus_tag="VKU03_06105"
     CDS             <1..134
                     /locus_tag="VKU03_06105"
                     /inference="COORDINATES: protein motif:HMM:NF019378.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1614 domain-containing protein"
                     /protein_id="HLJ70872.1"
                     /translation="GGAGTFDGIFLTGVLAVLIAGVPAAFRRKDDGKYGTTRSPWGR"
     gene            complement(196..1269)
                     /locus_tag="VKU03_06110"
     CDS             complement(196..1269)
                     /locus_tag="VKU03_06110"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016703 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of one atom of oxygen (internal
                     monooxygenases or internal mixed function oxidases)
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HLJ70873.1"
                     /translation="MPHPALARAEAFCAAYGLRVPVLLAPMAGACPPSLSIAVANAGG
                     LGACGALLMQPEAIKAAAAELRAGGNGAFQINLWIPDPPPPRDPAREAAVRAFLAQWG
                     PEVPAEAGDAKPPDFEAQCEAMLEAGPSIISSIMGLYPPAFVERMKARGVRWFATATT
                     VAEAKAAEAAGADAIVAQGMEAGGHRGAFDPAKAEAAMVGLFALLPAIADAVKVPVIA
                     TGGIADARGLAAALILGASAVQVGTAFLRSPEAKLPAAWADAIGRSAPEQTLVTRAFS
                     GRPGRSVATAYARAASGPEAPAPAPYPVQRGLTRSMREAAAKSGDIDRMQAWAGQSAA
                     LAQARPAGEIATEIWEGARRLLE"
     gene            complement(1321..1695)
                     /locus_tag="VKU03_06115"
     CDS             complement(1321..1695)
                     /locus_tag="VKU03_06115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SgcJ/EcaC family oxidoreductase"
                     /protein_id="HLJ70874.1"
                     /translation="MSEDERAIREVVETWMRATKAGDVATVLSLMTEDALFIVPGRPP
                     FGKDAFAAASDSMKDVRIDGSAEILEIQVAGDWAYLRNRVDVTMSPKDGPPVRRSGYT
                     LTIMRKERDGRWRLARDANLVT"
     gene            complement(1714..>2965)
                     /locus_tag="VKU03_06120"
     CDS             complement(1714..>2965)
                     /locus_tag="VKU03_06120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLJ70875.1"
                     /translation="TERGRLLVKLGQAIADHSADLAALESRDTGKPLKQGKADMVAAA
                     RYFEFYGTAADKLHGETIPFLNGYTIAIVRDPHGVTGHIIPWNYPAQIFGRSIAASLA
                     CGNSCVVKPAEDACLSVLRMAELARDVGFPDGALNVVAGLGGEAGAALSAHPDVNFIS
                     FTGSPQTGAAIQAAAAQNNVGVTMELGGKSPQVVFADADIEAAAPVLINAIIQNGGQT
                     CSAGSRVLIERKAYEAFSKIIAERFSKVRAGAHDKDLDMGAMINAKQQARVNGFLAKA
                     KQDGIKVVAEGALDKDAPKSGFYVTPTLFGDVARGNVLAHDEVFGPVLAMLPFEDEAD
                     AIALANGTPYGLIAAVWTQDAKRSIRVARAMRCGQVFVNGYGAGGGVELPFGGVKKSG
                     HGREKGFEALYEFCATKTIVINHG"
BASE COUNT          436 a         1022 c         1042 g          465 t
ORIGIN      
        1 tcggcggcgc cgggacgttc gacggcattt tcctcaccgg cgtgctggcc gtcctgatcg
       61 ccggcgtccc cgccgccttc cgccgaaagg atgacggaaa gtacggtacg acgcgctcgc
      121 cttgggggcg gtagcccctg tccggcgcca ttccggggaa tgacacaggg gagcggcgtg
      181 aagcgcgccc ctcggctact ccagtaacct ccgcgccccc tcccagatct ccgtcgcgat
      241 ctcgccggcc ggccgcgctt gcgccagggc ggcggattgc ccggcccagg cctgcatccg
      301 gtcgatgtcg ccgcttttcg ccgccgcctc gcgcatcgac cgggtgaggc cgcgctgcac
      361 cggatagggc gcgggcgcgg gcgcctccgg gccgctcgcc gcgcgcgcat aagcggtcgc
      421 gacgctgcgg ccgggccgcc cggaaaaggc gcgcgtcacc agggtctgct cgggcgcgct
      481 cctgccgatc gcgtcggccc aggccgccgg caatttggcc tccggactac gcaggaaggc
      541 ggtgccgacc tgcacggccg aggcgccgag gatcagcgcg gcggcgagac cccgcgcgtc
      601 ggcgattccg ccggtcgcga tcaccggaac cttcaccgcg tcggcgatcg ccggcagcag
      661 cgcgaacagg ccgaccatgg cggcttccgc tttcgcgggg tcgaaggcgc cgcgatggcc
      721 gcccgcctcc atgccttgcg cgacgatcgc gtcggcgccg gccgcctcgg cggctttggc
      781 ctcggcgacg gtcgtcgcgg tcgcgaacca gcgcacgccg cgcgccttca tcctctcgac
      841 gaaggccggc ggatagaggc ccatgatcga ggaaatgatc gacgggccgg cttcgagcat
      901 ggcttcgcat tgcgcttcga aatccggcgg cttggcgtcg ccggcctcgg ccggaacctc
      961 agggccccat tgggcgagaa aagcccgcac cgccgcctcg cgcgccgggt cgcgcggcgg
     1021 cggcggatcg ggaatccaga ggttgatttg aaaagcgcca ttgccgccgg cgcgaagctc
     1081 cgccgccgcg gccttgatcg cctcgggctg catcagcagc gcgccgcagg cgccgagccc
     1141 gccggcattg gcgacggcga tcgacagcga aggcggacag gccccggcca tcggcgcgag
     1201 cagaaccgga acgcgaagtc cgtaggcggc gcaaaaggcc tcggcgcggg cgagggcggg
     1261 atgcggcatg ttcggcctcc aaggcggcgc gcggcgtacg tttccttagc gggcttgctc
     1321 ttatgtgacg agattggcgt cgcgcgccag ccgccagcgg ccgtcgcgct ccttgcgcat
     1381 gatggtcagc gtatagccgg accggcggac aggcgggccg tccttggggc tcatcgtgac
     1441 gtcgacccgg ttgcgcaaat aggcccagtc gcccgccact tggatttcca ggatctcggc
     1501 cgagccgtcg atccggacgt ccttcatgct gtccgaggcg gcggcgaaag cgtctttgcc
     1561 gaaaggcggc cggccgggga cgatgaagag ggcgtcctcg gtcatcaggc tcagcaccgt
     1621 cgcgacgtcg ccggccttgg tcgcgcgcat ccaggtttcg acgacctcgc ggatcgcgcg
     1681 ctcgtcctcg ctcaactgga aagcctttcg ccgctagccg tgattgatca cgatcgtctt
     1741 cgtcgcgcag aattcgtaga gcgcctcgaa gcctttctcg cggccatggc cggatttctt
     1801 gaccccgccg aaaggcagct cgacgccgcc gccggcgcca tagccgttga cgaagacctg
     1861 gccgcagcgc atcgcgcggg cgacgcggat cgagcgcttc gcgtcctgcg tccagacggc
     1921 ggcgatcagg ccgtagggcg tgccgttggc gagcgcgatg gcgtcagcct cgtcctcgaa
     1981 gggcaacatc gccaggaccg ggccgaacac ttcgtcatgg gcgagaacat tgccgcgtgc
     2041 gacatcgccg aacagagtcg gcgtgacgta gaagccgctc ttcggcgcgt ccttatcgag
     2101 cgcgccctcc gcgacgacct tgatcccgtc ctgcttcgct ttcgcaagga agccgttcac
     2161 gcgcgcctgc tgcttggcgt tgatcatcgc acccatatcg aggtccttgt cgtgggcgcc
     2221 ggcgcgcact ttggaaaagc gctcggcgat aatcttggag aacgcctcgt acgccttgcg
     2281 ctcgatcagc acgcgcgagc cggccgagca ggtctggccg ccattctgga tgatggcgtt
     2341 gatgaggacc ggcgccgccg cctcgatatc ggcgtcggcg aagacgactt gcggcgactt
     2401 gccgccgagc tccatcgtca cgccgacatt gttctgcgcc gccgcggcct ggatcgccgc
     2461 gccggtttgc gggcttccgg tgaaagagat gaaattcacg tcgggatggg ccgacaacgc
     2521 cgcgcccgct tcgccgccaa gcccagcgac gacgttgagc gcgccgtcgg ggaatccgac
     2581 atcgcgggcg agttcggcca tgcggagcac gctgaggcag gcgtcttccg ccggcttgac
     2641 cacgcaggaa ttaccacacg cgagcgatgc ggcgatcgag cggccgaaaa tctgcgccgg
     2701 gtagttccag ggaatgatat ggccggtgac gccatgcggg tcgcggacga tggcgatcgt
     2761 atagccgttg aggaacggga tcgtctcgcc atgcagcttg tcggcggccg tgccatagaa
     2821 ctcgaaatag cgggccgcgg cgaccatatc ggccttgccc tgtttcagcg gcttgccggt
     2881 gtcgcgcgat tcgagcgcgg cgaggtcggc ggaatggtcg gcgatggctt ggccgagctt
     2941 gaccaggagg cggccgcgct cggtc
//
LOCUS       DATGJW010000199        19349 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_761911, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000199 DATGJW010000000
VERSION     DATGJW010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 19349)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19349)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19349
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_761911"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VKU03_06125"
     CDS             complement(<1..439)
                     /locus_tag="VKU03_06125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLJ70876.1"
                     /translation="MKIVTGLAGLALAGLMASTSLAADKPLFVYVSPGPIGVDDFLKL
                     GKAGTEQVAKELGGEAKTYESTDPTTQRQNLEAAAKAGAKIVIAIGFEFNDMLPDVAT
                     AYPNTKFLQIDSCPFSKMKPNIYCSVFREYEASFLTGAEAALTT"
     gene            complement(632..1096)
                     /locus_tag="VKU03_06130"
     CDS             complement(632..1096)
                     /locus_tag="VKU03_06130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013417890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLJ70877.1"
                     /translation="MSESTNETTFRAAREGFHWDGVETRRYKDEPNAPYREVTRQILF
                     SDPRLKGELRYFEVAPGGYTTLEKHEHMHAVLVLRGGGACLIGDDVRALKLNDLVTVP
                     PWTWHQFRAGKDEPLGFLCLVNAERDRPQLPSDSELAEMKRDPTVRAFLEGK"
     gene            complement(1089..2204)
                     /locus_tag="VKU03_06135"
     CDS             complement(1089..2204)
                     /locus_tag="VKU03_06135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016984 - ribulose-bisphosphate
                     carboxylase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RuBisCO large subunit C-terminal-like
                     domain-containing protein"
                     /protein_id="HLJ70878.1"
                     /translation="MSASRLIATYRLRADAATIEERARAIAIEQSVEAPLAAIRDKNI
                     LADIVGETRHIADRGGGVFDIEIALAVATIGGDAGQLLNMLFGNTSLHDDVALIAVDL
                     PEALVAAFDGPSHGLAGLRKRVKAARRPLTASALKPQGLSPDALAALAHNLALGGLDF
                     LKDDHGLANQAFSPFEERVPACAAAVRAASRATGHPTRYVPSLTGDRLAIERQIALAR
                     GEGIDTFLIAPAIAGPSTLQALRRAHPDLAFLAHPTLTGSRIAPDVYARLFRLFGADA
                     SIFPNYGGRFGYSEQACRGIADSLRKPWFGLAAAAPVPAGGMSLKRIPEMLDFYGPDA
                     MLLIGGALLAEPPNRLVAETRAFVRAVGALEHGAAHV"
     gene            2435..3643
                     /locus_tag="VKU03_06140"
     CDS             2435..3643
                     /locus_tag="VKU03_06140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932814.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ70879.1"
                     /translation="MDRRQLLVWSSAFALAPRVAFGDAIKPLKIGIMNDMSSVYADYQ
                     GVGSIVATKLAVDDYSQKLGVPVEIVSADHQNKPDIGSAIARRWFDEDGVDVIMDLPN
                     SSVALAVVNVATEKNKAAIGSGAGSSVLTGPKCSRNFIHWTYDTYALGHGLGKALTEQ
                     GGKTWFFIIADYAFGKDLKANCAAAVEAAGGKVLGEARHPLNTADFSSYLLQAQASGA
                     DIVGLANAGGDLNTCIKQAAEFGLTKKQRLAGLILNVTNIPSLGLAAVQNSTICTGFY
                     WDMNDGARAFAKRFQAAHPKKMMPNDMHAGMYSATAHLLKAMAVIKSAADGVKLIDQM
                     KAMPTDDPLFGRGSIRIDGRKLHPMYLLETKTVAESKGEWDYFKVLATIKPEDAFRPL
                     DKGGCPFVKA"
     gene            3887..5224
                     /locus_tag="VKU03_06145"
     CDS             3887..5224
                     /locus_tag="VKU03_06145"
                     /EC_number="1.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012253505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004412 - homoserine dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine dehydrogenase"
                     /protein_id="HLJ70880.1"
                     /translation="MSKPLRIGVAGLGVVGASLVRLLQRQAEALASRTGRAIVTTAVS
                     ARDRSRDRGLDLSGIGFFDDPRALATAESIDVFVELIGGADGPAYDSVKAALHSGKPV
                     VTANKALIARHGLELARLAEAKGLAFAFESSVAGGVPIVKTLREGLAGNSIRRIFGIL
                     NGTCNYILSKMETDGLSFAECLKEAQRLGYAEADPTFDIGGFDTAHKLAILTSLAFGA
                     AMDAEAIHVEGIETITQDDLSAADELGYRIKLLGVAERTAHGIERRVHPTMVAKSTAI
                     AQVMGVLNAVTIDADAVGELTLVGPGAGGDATASAVAADIVDIARGAAIQPFGRPTQT
                     LEEIERIPMRHHEGGYYVRLMVVDKPGAMAAIATRMGERKISLESIMQKGGSAMARRR
                     RTDPPPGAGEPDVVRVVLITHATSEQAIREALDLALADGVVTQKPQVIRIERE"
     gene            complement(5285..5809)
                     /locus_tag="VKU03_06150"
     CDS             complement(5285..5809)
                     /locus_tag="VKU03_06150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HLJ70881.1"
                     /translation="MNHDSYTDDYIRGILHTVKRIAMVGASNNPARPSYFVLKYLATR
                     GYQMLPINPGLAGKDIDGIRVYASLAEAPGPIDMVEIFRNSEAAGPIVDEALKLSPLP
                     KVIWMQLSVRNDAAAAKAEAAGLKVVMNRCPKIEYGRLSGEISWNGVNSRVLSAKKPV
                     RGGAGFQRLTIERP"
     gene            complement(6027..6839)
                     /locus_tag="VKU03_06155"
     CDS             complement(6027..6839)
                     /locus_tag="VKU03_06155"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006611046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HLJ70882.1"
                     /translation="MTLSIDQAQAPILAREDRGRVAKLILDRQSSRNSLSLDMIEALD
                     AAFAALAEDRAIRAVVLAARGPVFSSGHDLRDIRAHRNDKDRGRAYDEGLMARCSAMM
                     QAIVALPKPVIAAVEGVATAAGCQLVATCDLAIAGEKARFALPGINIGLFCSTPLVAV
                     GRAISRKAAMEMALTGELYSAEAALRFGLVNRVVAAGAAEDEAMKLAAGFAQRSAKTL
                     AIGKRAFYRQIEKPLDEAYALASAAMVQNLAERDADEGISAFLDKRPPRWEE"
     gene            complement(6921..7802)
                     /locus_tag="VKU03_06160"
     CDS             complement(6921..7802)
                     /locus_tag="VKU03_06160"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ70883.1"
                     /translation="MSQTASFAPLALLLAVGALLGLTANVVKLAAGVGWPPIAFLFWS
                     ALAAGFILLVATIARGEAPGLRRSDLAYYLVCGLLSIAAPNTLGFGAVAHVGASFIAL
                     CFALPPLLTYGLALTLRMERLKATRASGVACGLAGAAILAAAKTRGGGEDSLWIAGAL
                     ASPIFISFGNIYRTMRWPSGATALSLAPGMLIFGSLLIAPGLFAFGIPFGPARLAGAA
                     LWLLVGQTIIFAATYALYFLLQKLAGPVYLSQIGSVAAVIGGALAVLALGEPAGVSLF
                     VSAGLILLGVVLVNRGR"
     gene            7921..8442
                     /locus_tag="VKU03_06165"
     CDS             7921..8442
                     /locus_tag="VKU03_06165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ70884.1"
                     /translation="MSRYIMTESEPDRAEIALAQWRRERPDLDSFAMAVLGRLAETAL
                     VISRDRLAPLFARFGLQPGEFDVLATLRRSGAPYALTPTALYEAAMISSGGMTNRLDR
                     LEKEGLIERRPHPKDRRGLLVALTQKGLALIDEAVTAHVANERAILAAMSASEQAALY
                     ALLAKLLDGAKAG"
     gene            8439..9062
                     /locus_tag="VKU03_06170"
     CDS             8439..9062
                     /locus_tag="VKU03_06170"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205211.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ70885.1"
                     /translation="MNSRSQQSERKTKAARSTASAAVETSRLVFRPVDRQTWPDFAAL
                     FEERGGPKSCWCMVWRATPGEAKARDGASRRAQIEARVEAGAPIGLLAYCEAKPIAWC
                     SIAPRETYRPLGGPPAAPDEKIWSIVCFFIKRSWRGQGLAASLIEAAAAEAARRGATT
                     IEAYPVDPNSPSYRFMGFVPLFEAAGFREVGQAGARRRVMRRTAARS"
     gene            9364..10224
                     /locus_tag="VKU03_06175"
     CDS             9364..10224
                     /locus_tag="VKU03_06175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397664.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ70886.1"
                     /translation="MDDATKGWVNGLVGTAIFSASLPATKAAVAGFDPLFLASARGAI
                     AGALGLAALAAFRQKRPERGDVPALIVVALGVVIGFPLLTALALRTITSAHSLVFIGL
                     LPLMTALFGALRGGERPRPAFWFFSCLGAACVTGFAFSRGISALSVGDLLMVAAIIVC
                     GLGYAEGGRLSRKLGGWQVISWALALALPITFAAAIYVLPPSFQGVPPSAWLGLFYVT
                     LFSMLIGFVFWYRGLALGGIAAVGQLQLLQPFLGLFLAALLLGEEVGALALVTTAAVV
                     LCVAGARRYA"
     gene            10323..10787
                     /locus_tag="VKU03_06180"
     CDS             10323..10787
                     /locus_tag="VKU03_06180"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016790 - thiolester hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLJ70887.1"
                     /translation="MHITDAQRPTLALDCDEITAFLETEFPQMQIGRIFTIEEVGPMR
                     ARLRMIYHPSQLRPGGTISGPSIFALADVALYVAILAQIGRVKLAVTTNLNINFLAKP
                     APRDLIGEALLVKLGKRLAVGEVRIASAGEEVLAAHATGTYSIPPRGDDTVS"
     gene            10951..11421
                     /gene="rplM"
                     /locus_tag="VKU03_06185"
     CDS             10951..11421
                     /gene="rplM"
                     /locus_tag="VKU03_06185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HLJ70888.1"
                     /translation="MFGKTTSTKPADVEKKWLLIDAKGLVVGRLATIVAMRLRGKHKP
                     SFTPHVDDGDNVIVVNAEKVVFTGRKRDQKVYHHYTGYIGGIKERSAKSILEGRYPER
                     IVEKAVERMLPRGPLGRKQLGNLRVYKGPDHPHAAQNPVALDVASLNPKNRRSA"
     gene            11427..11906
                     /gene="rpsI"
                     /locus_tag="VKU03_06190"
     CDS             11427..11906
                     /gene="rpsI"
                     /locus_tag="VKU03_06190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HLJ70889.1"
                     /translation="MAETLSSLADLKDATAAPAPEAPHYVQKLDKHGRAYATGKRKNA
                     VARVWIKPGRGQATVNGRALDAYFARPVLRMILNQPLVIANRLTQYDLIINVSGGGLS
                     GQAGAVRHGIAKALTNYEPELRPILKKEGFLTRDSRVVERKKYGHKKARRSFQFSKR"
     gene            12095..13357
                     /locus_tag="VKU03_06195"
     CDS             12095..13357
                     /locus_tag="VKU03_06195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70890.1"
                     /translation="MRGRSKGLAAASGSLAAIIVAASFFLTPHQARAWPAGETGDVAI
                     DDFSTTDKDGGKIVIKHIEFTNTNLEKEEVQKLLTPATPKDEALELVKKLKADKISMP
                     SIELSPKAGGLVRLTGFEASDIDEGRVGKLSIAGIDGGGEQNGAKLAIKSGALALEDA
                     DLAEVLKATGGAGGAPKGRLGHLTWQSVDIVAPDDTTPGKSIHVAFGSFELKSDYDGD
                     VMKQGSTTLNGLLVEPAPGSEFATELASLGYSKIELGAAVGAHYEAGAKTLSLDQFMI
                     EGVQMGSVGLKADFADIEPALFTGDDGARMQALLGGSIASLEIKLVNSGLFEKSLAFY
                     AKQQGATPEALKQQIAATAAQMVPLMLGGAPDSLKVAAEAQKFINSPKNLTVSLKAKG
                     APLKAADFMAGDPSAILGKVSISAIANQ"
     gene            13519..14466
                     /gene="argC"
                     /locus_tag="VKU03_06200"
     CDS             13519..14466
                     /gene="argC"
                     /locus_tag="VKU03_06200"
                     /EC_number="1.2.1.38"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003942 -
                     N-acetyl-gamma-glutamyl-phosphate reductase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyl-gamma-glutamyl-phosphate reductase"
                     /protein_id="HLJ70891.1"
                     /translation="MPAKIFIDGEAGTTGLQIREKLAGLPGVALVSLPPEQRKDAEAK
                     KRLLAEADLVILCLPDSAARETVALIDGLAGPQPRIIDASTAHRVAPGWTYGFPELTA
                     GQAAAIAAAKRVANPGCYATGAIALLRPLVEAGLIPASHRVTINAVSGYSGGGKAMIA
                     AYESGSAPAFELYGLAGEHKHLPEIVAHSGLARRPIFVPSVGNFKQGMLVSIPLWLDE
                     LPGRPDHAALAAALAKHYAGSAYVSVVPCGDQSHASGRLDALSLNGTNEMQLHIFGDP
                     TQRQAVLVAKLDNLGKGASGAAVQNLKLMLGLEKAARAA"
     gene            14542..15120
                     /locus_tag="VKU03_06205"
     CDS             14542..15120
                     /locus_tag="VKU03_06205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611321.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HLJ70892.1"
                     /translation="MSVPVSRAAEGLARRAFTIADLERMAEVGLIAPDERLELIGGEI
                     VPMSPKGSRHETIKAAINFHWGQRCPAGFAFIPETGLRLDERTYIEPDFIVFERKRRL
                     ADIRGPDVLLAVEVADSSLRYDLDRKPLVYAGFGVRELWVIDAERHAVHRHAEPSPVG
                     YKRIEEYGGADRLTPLHAPAPFAFALDELEPI"
     gene            complement(15127..15459)
                     /locus_tag="VKU03_06210"
     CDS             complement(15127..15459)
                     /locus_tag="VKU03_06210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70893.1"
                     /translation="MTFNAAAPRRRPFLSLLGAALAALYLAAIIAGYVIYVQNAGQWL
                     ADLTLLLIAMPFTWTMSVLTSGAFSMSGDETGKVVAAALFCCALAYLAGAITEGLLRA
                     TWRLARRG"
     gene            complement(15456..16310)
                     /locus_tag="VKU03_06215"
     CDS             complement(15456..16310)
                     /locus_tag="VKU03_06215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLJ70894.1"
                     /translation="MKLVRYGKPGKEKPGLIDGAGALRDLSGVIDDLAGEALSPRSLA
                     KIAKLKPASLPAVRGEPRLGACVGQVGNFIAVGLNYADHAAETGAAIPKEPILFNKAP
                     SCIVGPNDDVVIPKASVKTDWEVELAVVIGRRASYVADHEAFDYIAGFCVCNDVSERE
                     FQAERGGQWMKGKGCATFGPLGPWLVTPDEIPDVQKLSMWLDVNGQRMQNGSTSTMIF
                     DVKMLVSYISHFMVLLPGDVITTGTPPGVGLGMKPPRFLKAGDTVALGIEGLGEQRQR
                     FVAWTKEA"
     gene            16832..17440
                     /locus_tag="VKU03_06220"
     CDS             16832..17440
                     /locus_tag="VKU03_06220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HLJ70895.1"
                     /translation="MADQERIALFIDGANLYATAKSLGFDIDYKRLLREFQSRGRFVR
                     AFYYTALVEDQEYSSIRPLVDWLDYNGYSVVTKPTKEFVDSTGRRKVKGNMDIELAVN
                     AMEMAAHIDHMVLFSGDGDFRSLVEAVQRRGVRVSVVSTVQTQPAMVADELRRQADEF
                     IDLASLASRIGRDPSDRPPRHADARRGPPQQAGLVNDDFVDG"
     gene            17418..18134
                     /locus_tag="VKU03_06225"
     CDS             17418..18134
                     /locus_tag="VKU03_06225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLJ70896.1"
                     /translation="MMISSTAEEALAPAPQSKLPFDVSPPRDCPACPRLAEFRAANRA
                     AHPDWRNEPVASWGAADSRLLIVGLAPGLRGANRTGRPFTGDFAGDLLYETLLAFGFA
                     RGVYDRRPDDGLELVDCAIVNAVRCVPPQNKPTPQEIATCRAYLAGAIGALKNLEAIV
                     ALGRIAHDSALRAMGLPLAGFPFAHGAAHQLPNGTALYDSYHCSRYNTNTGVLTPKMF
                     AEVFAAVRRRLGAPGRAGLP"
     gene            18215..18604
                     /gene="rpoZ"
                     /locus_tag="VKU03_06230"
     CDS             18215..18604
                     /gene="rpoZ"
                     /locus_tag="VKU03_06230"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit omega"
                     /protein_id="HLJ70897.1"
                     /translation="MARVTVEDCIDKVENRFDLVLMASHRARMLSAGAQITVPRDNDK
                     NPVVALREIADQRLSPEDLKEDLIHSLQKNVEVDEPEAETAPPLVSADIAPGEAGDAQ
                     FDRMTEEDLLRGLEGLAPPPEPEEEAE"
     gene            18980..>19349
                     /locus_tag="VKU03_06235"
     CDS             18980..>19349
                     /locus_tag="VKU03_06235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010971879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cold shock domain-containing protein"
                     /protein_id="HLJ70898.1"
                     /translation="MRLDIREQAADAVEPPVGLVEVAGYVKWFDVGKGYGFIVPDGGK
                     PDVLIHVTVLRRDGFQTVHEGARVVAEAVQREKGLQVFRVISVEPGDGSHPSQLPPAR
                     THVQVTPTSGFEIVIVKWFNR"
BASE COUNT         3427 a         6256 c         6313 g         3353 t
ORIGIN      
        1 tcgtcgtcaa agccgcttcg gcgccggtga ggaagctcgc ctcatattcg cggaagaccg
       61 agcaatagat gttcggcttc atcttgctga acgggcagga atcgatctgc aaaaacttgg
      121 tgttgggata ggcggtcgcc acgtccggca gcatgtcgtt gaactcgaag ccgatggcga
      181 tgacgatctt ggcgccggct ttggccgccg cttcgagatt ctggcgctgc gtcgtcggat
      241 cggtgctctc gtaagtcttg gcttcgccgc cgagctcctt ggcgacctgc tccgtgccgg
      301 ctttgccgag cttgaggaaa tcgtcgacgc cgatcgggcc gggagagaca tagacgaaaa
      361 gcggcttgtc cgccgccaag cttgtcgagg ccataaggcc cgcgagcgcg agcccggcca
      421 atccagtcac gattttcatt cgttcttctc cccgctctgg cgaaaggctc cagcctggag
      481 tccctcgcgc gccgcctcaa agcaaattcc agcagaaatc ggcagggcgc gcaacgggtt
      541 agaccttgtc attcgcgcgc aagcgggaat ccaggctggg catgctttcg aacgcgaaaa
      601 ctggattccc gctttcgcgg gaatgacaag ctcacttccc ttcgagaaat gcgcgcaccg
      661 tcggatcgcg cttcatctcg gcgagctcgc tgtcgctcgg caattgcggg cggtcgcgct
      721 cggcgttgac gaggcagaga aagccgagcg gctcgtcctt cccggcgcgg aactgatgcc
      781 atgtccaagg cggcacggtg acgagatcgt tgagcttgag cgcgcgcaca tcgtcgccga
      841 tgaggcatgc gccgccgccg cgcagcacca ggacggcgtg catatgttcg tgcttttcga
      901 gcgtcgtata gccgccgggc gcgacttcga aatagcgcag ctcgccctta aggcgcggat
      961 cgctgaacag gatctgccgc gtcacctccc ggtacggcgc attcggttcg tccttgtagc
     1021 gtcgcgtctc gacgccgtcc caatgaaacc cttcgcgcgc ggctcggaac gtcgtctcgt
     1081 tcgtactctc agacatgcgc cgctccgtgt tcgagggcgc cgacggcgcg cacgaaggcg
     1141 cgcgtctcgg cgacgagtct attcggcggc tcggcgagca aagcgccgcc gatcagcagc
     1201 atcgcgtccg gcccgtagaa gtcgagcatt tccgggatgc gcttgaggct catgccgccg
     1261 gccgggaccg gcgctgcggc cgcaaggccg aaccagggct tgcgcaaaga atcggcgatg
     1321 ccgcggcagg cctgctcgga atagccgaac cggccgccgt aattggggaa gatcgacgca
     1381 tcggcgccga acagccggaa caggcgggca tagacgtccg gcgcgatgcg cgagccggtc
     1441 aaggtcggat gggcgagaaa ggccagatcg ggatgggcgc ggcgcaaggc ttgcagggtc
     1501 gaaggcccgg cgatcgccgg cgcgatcagg aacgtatcga tcccttcgcc gcgggcgagc
     1561 gcgatctgcc gctcgatcgc caggcggtcg cctgtgaggc tcgggacata gcgggtcgga
     1621 tggccggtcg cgcgcgaagc ggcgcgcacc gccgcggcgc aggcggggac ccgctcttcg
     1681 aaaggcgaga aagcctgatt ggcgaggccg tgatcgtctt tgaggaaatc gaggccgccc
     1741 agcgccaaat tatgggcgag cgccgccagg gcgtcgggcg acagtccttg cggtttcaaa
     1801 gccgaggcgg tcagcggccg ccgcgcagcc ttgacgcgct tgcgcaagcc ggcaagcccg
     1861 tggctggggc cgtcgaacgc cgcgaccagg gcctccggca gatcgacagc gatcaaggcc
     1921 acgtcgtcat gcaatgacgt gttgccgaac agcatgttga gcaattgccc ggcgtcgccg
     1981 ccgatcgtcg cgacggcaag cgcgatctcg atgtcgaaca cgccgccgcc gcgatcggcg
     2041 atatggcgcg tttcgccgac aatatcggcg agaatgttct tgtcgcgaat ggccgccagc
     2101 ggcgcctcga cgctctgctc gatcgcgatc gcccgcgccc gctcttcgat ggtcgctgcg
     2161 tcggcgcgaa gtctataggt ggcgatcagc ctcgaagcgc tcatcaagtc cccgtctcgc
     2221 cggtctgtcc ggcggggtgg ctctacgcca tcgccgcggg cgctggcaat ctccgccagg
     2281 caaaagggct tttgccctcg cgcgatcttg aacggaagaa gattatctcg tataactatt
     2341 ccttgcgggc ttgctttggc gcgcggggcg cattgcgccg ggaaaacgaa tagcgccggc
     2401 ccgcggagtg gggcggcgcg aagggaggag cgtcatggat cgtcggcaat tgctggtttg
     2461 gagctcggct ttcgcgctcg cgccgcgcgt cgctttcggc gacgccatca agccgctcaa
     2521 gatcggcatt atgaacgaca tgtcgagcgt ctatgccgat taccagggcg tcggctcgat
     2581 cgtcgcgacc aagctcgccg tcgacgatta ttcccaaaag ctcggcgtgc cggtcgagat
     2641 cgtctccgcc gatcaccaga acaagccgga tatcggcagc gccatcgccc ggcgctggtt
     2701 cgacgaggac ggcgtcgacg tgatcatgga cctgccgaat tcctccgtcg cgcttgccgt
     2761 ggtcaatgtc gcgaccgaga aaaacaaggc ggcgatcggc tcgggggcgg gcagctccgt
     2821 gctgaccggc ccgaaatgct cgaggaattt catccactgg acctacgaca cctatgcgct
     2881 cggccacggc ctcggcaagg cgctgaccga gcagggcggc aagacctggt tcttcatcat
     2941 cgccgattac gccttcggca aggacttgaa ggcgaattgc gcagcggcgg tcgaggcggc
     3001 gggcggcaag gtgctgggcg aggcgcgcca tccgctcaat accgccgact tttcttcgta
     3061 ccttctgcaa gcgcaagctt caggcgccga cattgtcggc ctcgccaatg ccggcggcga
     3121 tctgaacacc tgcatcaagc aggcggcgga attcggcctg accaagaagc agcgcctcgc
     3181 cggactcatc ctgaacgtca ccaacatccc ctcgctcggc ctcgcggcgg tgcagaattc
     3241 gaccatctgc accggcttct attgggacat gaacgacggc gcgcgcgcct tcgccaagcg
     3301 tttccaggcc gcccatccca agaagatgat gccgaacgat atgcatgccg gcatgtattc
     3361 ggcgaccgcc catctcctca aagcgatggc ggtgatcaag agcgccgccg acggagtcaa
     3421 actgatcgac cagatgaagg cgatgccgac cgacgatccg cttttcggca ggggctcgat
     3481 ccgcatcgac ggccgcaagc tgcacccgat gtatcttctc gagaccaaga cggtcgccga
     3541 gtcgaagggc gagtgggact atttcaaggt gctggcgacg atcaagcccg aagacgcgtt
     3601 ccgcccgctg gataaaggcg ggtgtccgtt cgtgaaggcg tgacgtaccc tcgccccgcg
     3661 aagcggggag agggtggccg cgaagcggcc gggtgagggg cagcgccgac gctggcgatt
     3721 agcgtccgcc tgggcgcatg tacttttgag tcctgcgcag cccctcatcc ggcccttcgg
     3781 gccaccttct ccccgcaagg cggggagaag ggcgcgccct tcttgccccc gccgcgcgtt
     3841 tcaggcaaaa cccggcctca attcggggcg ttcgatcgag gccgtcatgt cgaaaccttt
     3901 gcgcatcggc gtcgccggcc tgggcgtggt cggcgccagc ctcgtgcggt tgttgcagcg
     3961 gcaggcggag gcccttgcga gccgcaccgg ccgcgcgatc gtgacaaccg ctgtgtcggc
     4021 ccgcgatcgt tcgcgcgacc gcggcctcga tctgagcggg atcggctttt tcgacgatcc
     4081 gcgcgccctg gcgaccgcgg aatcgatcga cgtcttcgtc gagctgatcg gcggcgccga
     4141 cgggccggcc tatgattccg tcaaggcggc gctgcacagc ggcaagccgg tcgtcaccgc
     4201 caacaaggcg ctgatcgcgc gccacggcct ggagcttgcg cggctcgccg aggcgaaggg
     4261 cttggccttc gccttcgaat cttcggtcgc cggcggcgtg ccgatcgtca agaccctgcg
     4321 cgagggcctc gccggcaatt cgatccgccg catcttcggc attctcaacg gcacctgcaa
     4381 ttacatcctc tccaaaatgg agacggacgg gctctccttc gcagaatgcc tgaaggaggc
     4441 gcaaaggctc ggctatgccg aggccgatcc gactttcgac attggcggct tcgacacggc
     4501 ccataagctt gcgatcctca cctcgctcgc cttcggcgcg gcgatggatg cggaggcgat
     4561 ccatgtcgaa ggcatcgaga cgatcaccca ggacgatctc tccgccgccg acgaactcgg
     4621 ctaccggatc aagctgctcg gcgtcgccga gcgcacggcg catgggatcg agcggcgcgt
     4681 ccatccgacc atggtcgcca aatcgacggc gatcgcccag gtcatgggcg tgctcaacgc
     4741 ggtgacgatc gacgccgacg cggtcggcga actgacgctg gtcggcccgg gggctggcgg
     4801 cgacgccacc gcctcggcgg tcgccgccga tattgtcgac atcgcccgcg gcgcggcgat
     4861 ccagcccttc ggccggccga cgcagacgct cgaagagatc gagcgcatcc cgatgcggca
     4921 tcacgagggc ggctactatg tgcgcctgat ggtggtcgac aagcccggcg ccatggcggc
     4981 gatcgccacc cgcatgggcg agcgcaagat ttcgctggaa tcgatcatgc agaaaggcgg
     5041 ttcggcgatg gcgcgccgcc gccgcaccga cccgccgccg ggcgccggcg agccggacgt
     5101 cgtgcgcgtc gtgctgatca cgcacgcgac gagcgagcag gcgatccgcg aggcgctgga
     5161 cctggcgctg gccgacggcg tggtgacgca gaagccgcag gtgatccgga tcgagcggga
     5221 ataaggaccg acgcccttta tcgccattgc ttcgtcgctg cgcgcctcgc aatgacggag
     5281 gacgtcatgg ccgttcgatc gtcagcctct gaaatcccgc cccgccgcgc accggcttct
     5341 tggccgacag cacgcgcgaa ttgacgccgt tccaggagat ttcgccggag agacggccgt
     5401 attcgatttt cgggcagcga ttcatcacga ctttgagccc ggccgcctcg gctttggccg
     5461 ccgcggcgtc gttgcgcacc gagagctgca tccagatcac cttcggcaag gggctcagct
     5521 tgagcgcctc gtcgacgatc ggcccggccg cctcggaatt gcggaagatt tcgaccatgt
     5581 cgatcggccc gggcgcctcg gcgagcgagg cgtagaccct gatcccgtct atgtccttac
     5641 cggcgaggcc cggattgatc ggcagcattt gatagccgcg cgtcgcgagg tatttcagaa
     5701 caaagtacga tggtcgcgcc ggattgttcg acgcgccgac catggcgatg cgcttcacgg
     5761 tatggagaat gccgcggata taatcgtcgg tataggaatc gtggttcatg gctcaacagg
     5821 aagaggcgga gatccccttc tccccgcttc gcgaggagaa ggtgggccga tgggccggat
     5881 gaggggctgc gcggcggttg aagatgagcg aaagtacggg gaggctaacg cccggcgccg
     5941 gcgctgcccc tcacccggcg gcttcgccgc caccctctcc ccgcttcgcg gggcgagggg
     6001 gaaggcgcgc cttatggacc tacgcatcac tcttcccatc gcggcggccg cttgtcgaga
     6061 aaagcggata tgccttcgtc ggcgtcgcgc tcagcgagat tctggaccat cgccgcggag
     6121 gcgagggcgt aggcttcgtc cagcggcttt tcgatctggc gatagaaggc gcgcttgccg
     6181 atggcgagcg tcttggccga gcgctgggcg aagccggcgg caagtttcat cgcttcgtct
     6241 tcggcggcgc cggcggcgac cacgcggttg accaggccga agcgcagggc ggcttccgcc
     6301 gagtagagct cgccggtcaa ggccatttcc atcgccgctt tccgcgagat cgcgcggccg
     6361 accgcgacaa gcggcgtcga gcagaagagg ccgatattga tccccggcaa agcaaagcgc
     6421 gccttctcgc cggcaatggc gagatcgcac gtcgcgacga gctggcagcc ggccgccgtg
     6481 gcgacgccct cgaccgccgc gatgaccggc ttcggcaaag cgacgatcgc ctgcatcatc
     6541 gcagagcagc gcgccatcag gccctcgtca taggcgcggc cgcgatcctt gtcgttgcga
     6601 tgggcgcgga tgtcgcgcaa atcgtggccg gaggaaaaaa ccggcccgcg cgcggcgaga
     6661 accacggcgc gaatcgcgcg gtcctcggca agggccgcga aagccgcgtc gagcgcttcg
     6721 atcatatcga gcgacaggct gttgcggctc gattgccggt cgaggatcag cttggcgaca
     6781 cggccgcgat cttcgcgggc gaggatcggg gcttgggctt ggtcgatcga aagggtcata
     6841 gccgcaatgt cgtctctctg tcggcgccga tcaagccggg ggccagaggg gcagcagcgc
     6901 gcgcgaagcg cggctccaaa tcagcgtccg cgattgacca gcaccacgcc gaggagaatg
     6961 aggcccgcgg agacgaaaag agagacgccc gccggttcgc ccagagctag aacggcgagc
     7021 gctccgccga tcacggcggc gacggagccg atctggctga gatagaccgg tccggcgagt
     7081 ttttgcagaa gaaagtagag cgcgtaggtg gcggcgaaga tgatcgtctg gccgacaaga
     7141 agccacagcg ccgcgccggc gaggcgcgcc gggccgaaag gaattccgaa ggcgaagagg
     7201 ccgggcgcga tcagcagcga gccgaaaatc agcatgcccg gggcgagcga aagcgccgtc
     7261 gcgccggagg gccagcgcat cgtccggtag atattgccga aagagatgaa aatcgggctc
     7321 gccagcgcgc cggcgatcca aagcgaatcc tcgccgccgc ctcttgtctt ggcggcggcg
     7381 aggatcgcgg cgccggcaag gccgcaagcg acgccgctgg cgcgcgtcgc tttgagccgc
     7441 tccatgcgga gcgtcagagc caggccatag gtcaagagcg gcggcagcgc gaagcagaga
     7501 gcgatgaagc tcgcgccgac atgggcgacc gcgccgaagc cgagcgtgtt cggggcggcg
     7561 atgctgagca gcccgcagac gagataatag gcgaggtcgg atcgtctcaa tcccggcgct
     7621 tcgccgcgcg cgatcgtcgc gacgagcagg atgaagccgg cggcgagggc ggaccagaac
     7681 agaaaggcga tcggcggcca gccgacgccc gccgccagtt tcaccacatt cgcagtaagg
     7741 ccgagcagag cgccgacggc cagaagcaac gcgagcggcg cgaaagaggc ggtctgcgac
     7801 acggaccctt ctgcaaaacg gtaagtcatc acgcgccctc gacaatcgag ctgaccgaaa
     7861 cgccttttcc gcttggcgct aaggggtccc tgcgttacta tcttgctata gagtatctta
     7921 atgtcaagat atattatgac ggagagcgag ccggaccggg ccgagattgc tctggcgcaa
     7981 tggcgccggg agcggccgga cctcgattcc ttcgccatgg ccgtgctcgg ccgtctcgcc
     8041 gagaccgcct tggtgatttc gcgcgatcgc ttggcgccgc tcttcgcccg cttcgggctg
     8101 caacctggcg aattcgacgt cctcgccacc ttacgccgct ccggcgcgcc ctacgccttg
     8161 accccgacag cgctttacga ggcggcgatg atctcatccg gcgggatgac caatcgcctc
     8221 gaccggctcg aaaaagaagg gctgatcgag aggcggccac atccaaaaga ccggcgcggc
     8281 ctgcttgtcg cgctgacgca gaaaggcttg gctttgatcg acgaggcggt cacggcccat
     8341 gtcgcgaacg agcgcgcaat tctcgccgcc atgagcgcga gcgagcaggc ggcgctctat
     8401 gccttgctcg ccaagcttct cgacggcgcg aaggcgggat gaactctcgc tcccagcaat
     8461 cagagcgaaa gacgaaagcg gctcggtcca ccgcgtccgc cgcggtcgag acgtcgcggc
     8521 tggtttttcg accggtcgat cgccagacct ggcccgattt cgccgcgctc ttcgaggagc
     8581 ggggcgggcc gaaaagctgc tggtgcatgg tatggcgcgc cactcccggt gaagcgaagg
     8641 cgcgggacgg cgccagccgt agggcgcaga tcgaggcgcg cgtcgaggcg ggcgcgccga
     8701 tcgggcttct tgcctattgc gaggccaagc ccatcgcctg gtgttcaatc gcgccgcgcg
     8761 agacctatcg tccgctcggc ggcccgcccg ccgctccgga cgagaagata tggtcgatcg
     8821 tatgcttctt catcaagcgg agctggcgcg gccaaggctt ggccgcttca ttgatcgaag
     8881 ccgcggccgc ggaagcggca agacgcggcg ccacgacgat cgaggcctat ccggtcgatc
     8941 cgaattcgcc gagctatcgc tttatgggct tcgtaccgct gttcgaagcg gccggatttc
     9001 gcgaagtcgg gcaggccggg gcccggcggc gcgtgatgcg gaggacggcg gctcgttcat
     9061 agaacgaagg gctcagcgcc gaacatttga tcgatggcgg gaaatcggct ttgactgccg
     9121 gcgcgtcgcc atcctttgaa agcgcaagaa cgccgcctcc gccgggaagg gcgcaatgac
     9181 caacggcttt cgccatgaaa tgccccttgc ttcgcccggt gcgccttttg gttcgtgctc
     9241 gattggctcg ccgcgtgagc ccgtgctgcg cgcggcgtca tgacagttcg ctgaaacctg
     9301 accggattgt ctctggcgat tcgactgtcg ctgtccgaat gggcggcgat aggaggctgc
     9361 ggcatggacg atgcgacgaa aggctgggtc aacggccttg tcggtacggc gattttcagc
     9421 gcctcgctgc ccgcgaccaa ggcggctgtg gcaggcttcg atcctttgtt cctcgcctcg
     9481 gcccgcggcg cgattgccgg cgcgcttggt ttggcggcgc ttgcagcctt tcgtcaaaag
     9541 agacccgagc gcggcgatgt ccctgcgctc attgtcgtcg cgctcggcgt ggtcattggc
     9601 ttcccgttgc tcaccgcgct ggcgctacgc acgatcactt ccgcccattc ccttgtcttc
     9661 atcggtcttc tgccattgat gaccgcgctc tttggcgcct tgcgcggcgg cgagcgtccg
     9721 cgtccggctt tctggttttt ctcttgtctc ggcgctgcat gcgtgacggg atttgctttt
     9781 tcgcgtggaa tttcggcgct gtcggtcggc gacttactga tggtggccgc gatcatcgtt
     9841 tgtggcctcg gctatgccga aggcggccgg ctttcgcgca agctgggcgg ctggcaggtg
     9901 atctcgtggg cgctggcctt ggcgctgccg atcacgttcg cagcggccat ctatgttctg
     9961 ccgccctcat tccaaggcgt cccgccttcc gcctggctcg gccttttcta cgttacgctg
    10021 ttcagcatgc tgatcgggtt cgtcttctgg tatcgcggcc tcgcattggg cgggatcgcg
    10081 gcggtcgggc agttgcaact cctccaaccc tttctcggtc tgttccttgc cgcattgctg
    10141 cttggcgagg aggttggcgc gctcgccctg gtcacgacgg ccgccgtcgt cctttgcgtg
    10201 gccggcgcaa ggcgatacgc ttagcgcgcc ctgagcgcgc cctcgtgagg gcggtcgctt
    10261 ggtttgtgaa ggcgcgctgg tcgcgctctt tggatcgtac ctttgttctg ggaactctct
    10321 caatgcatat aacggatgcg cagcgcccaa cgctcgccct cgactgcgac gaaatcacgg
    10381 ccttcctcga aacggaattc ccgcaaatgc agatcggccg catctttacg atcgaagagg
    10441 tcgggccgat gcgggcgcgg ctgcgcatga tctatcatcc aagccagctt cggcccggcg
    10501 gcacgatttc cgggccgtcg attttcgcgc tggcggacgt cgcgctttac gtcgcgatcc
    10561 tcgcccagat tggccgggtc aagcttgcgg tgacgaccaa tctcaacatc aattttttgg
    10621 cgaaaccagc gccgcgcgac ctgatcggcg aagctttgct cgtcaagctc ggaaaaaggc
    10681 tcgcggtcgg cgaagtgcgc atcgcatcgg cgggcgagga ggttctcgcc gcccatgcga
    10741 cgggaaccta ttcgatcccg ccacgcggcg acgatacggt atcataatac cgcataacgc
    10801 attcgccttt attttcagct cattcgacgc ctgagagacc ccttctctgc aaaagtgcgg
    10861 ttgacgcgag gctggttgcg cccctataag ctcggacttc ggcgcgcctt tgagcgcgcc
    10921 ttttcgatca agacggattt caaaaccctc atgttcggca aaaccacttc gaccaaaccg
    10981 gccgatgtcg agaagaaatg gcttctgatc gacgccaagg gcctcgtcgt cgggcgcctc
    11041 gccacgatcg tcgcgatgcg cttgcgcggc aagcacaagc cctccttcac gccgcatgtc
    11101 gatgacggcg acaatgtcat cgtcgtcaac gccgagaagg tcgtcttcac cggccgcaag
    11161 cgcgaccaga aagtctacca tcactacacc ggctatatcg gcggcatcaa agagcgctcg
    11221 gccaagtcga tcctcgaagg gcgctatccc gagcgcatcg tcgaaaaagc ggtcgagcgc
    11281 atgctgccgc gcgggccgct cggccgaaaa cagctcggca atcttcgcgt ctataagggc
    11341 cccgatcacc cgcacgccgc ccaaaacccg gtcgccctcg acgtggcgag cctgaatccg
    11401 aaaaaccgcc ggagcgcctg attcccatgg ctgagaccct ctcctcgctc gccgatctca
    11461 aagacgcgac tgccgcgccg gcgccggaag cgccgcacta cgtgcagaag ctcgacaagc
    11521 acggccgcgc ctatgcgacc ggcaagcgca agaacgcggt cgcccgcgtc tggatcaagc
    11581 ccggtcgcgg ccaggcgacg gtgaacggcc gggcgctcga cgcttatttc gcgcgcccgg
    11641 ttctgcgcat gatcctcaac cagccgctcg tcatcgccaa tcggctgacc caatacgatc
    11701 tgatcatcaa tgtctcgggc ggcggccttt ccggccaggc cggcgcggtg cgtcacggca
    11761 tcgccaaggc gctgaccaat tacgagccgg agctcaggcc gatcctgaag aaggagggct
    11821 tcctgacccg cgattcccgc gtcgtcgagc gcaagaaata cggccacaag aaagcccgcc
    11881 gcagcttcca gttctcgaag cgttagccgc gattggattt cgacgaagaa aggcggcttc
    11941 tgccgccttt ctttttgccg ggagcgcccg gccggccatc gacaccggcg gcgacttcgc
    12001 gcatgatcaa agaaacagcg ctttttgagc gcggcgggcc gccaaacacg gcgcgggtcg
    12061 gcgcgcaacg gggattctgg agggagtaga ggggatgcgc ggacgttcta agggcctggc
    12121 ggccgcttcc ggcagcctcg cggcgatcat tgtcgccgcg agcttttttt tgacgcctca
    12181 tcaggcgcgc gcctggccgg cgggagaaac cggcgatgtt gcgatcgacg atttttcgac
    12241 taccgataag gatggcggca aaatcgtcat caagcatatc gaattcacca ataccaatct
    12301 cgaaaaggaa gaagtccaaa agctgctgac gccggctacg ccgaaggacg aggcgctcga
    12361 actcgtcaag aagctgaaag cggacaaaat ctcgatgccg tccatcgagc tttcgccgaa
    12421 agcgggcggc cttgtccgcc taaccggctt cgaggcgagc gacatcgacg agggcagggt
    12481 cggcaagctg tcgatcgccg gcattgacgg cggcggcgag caaaacggcg cgaagctcgc
    12541 gatcaaatcc ggcgcgctgg cgctcgaaga cgccgatctc gccgaagtgc tgaaagcgac
    12601 gggcggggct ggcggcgcgc cgaaagggcg gctgggccac ttgacctggc agagcgtcga
    12661 cattgtcgcg cccgacgata cgacgcccgg caagtcgatc catgtcgcct tcggctcgtt
    12721 cgagctgaag agcgattacg acggcgacgt gatgaagcaa ggctcgacca cgctgaatgg
    12781 ccttctcgtc gagccggcgc ccggatccga attcgccacg gaattggctt cgctcggcta
    12841 ttccaagatc gaactcggcg ccgcggtcgg cgcccattac gaagccggcg ccaagaccct
    12901 gtcgctcgat cagttcatga tcgaaggcgt gcaaatgggc tcggtcgggc tgaaggccga
    12961 cttcgccgat atcgagccgg ctctcttcac cggcgacgac ggcgcgcgca tgcaggcttt
    13021 gctcggcggc agcatcgcct cgctcgaaat caagctcgtc aattccggcc ttttcgaaaa
    13081 gagtctggct ttctacgcca agcagcaagg cgcgacgccc gaggcgctca agcagcagat
    13141 tgcggcgacc gcggcgcaga tggtgccgct gatgctcggc ggcgcgcccg attcgctgaa
    13201 agtcgccgcg gaagcgcaga aattcatcaa ttcgccgaag aacctcacag tgtcgctgaa
    13261 agccaaaggc gcgccactga aggccgccga tttcatggcc ggcgatccga gcgccattct
    13321 cggcaaggtc agcatttccg cgatcgccaa ccaataaggc ggcgaggctt gacattcggc
    13381 ggcgcccggg ccatctgcgc gggcatgatc agctcaggcg ccgcacgaat ccgacgattt
    13441 tgatcgccgc ccgcgacgag acgagctcgt cgccgcaagc ctcgcttcaa gcgcaacgcg
    13501 ccgccttcag ggttgatcat gccagccaag attttcattg acggcgaagc cggcacgacc
    13561 ggcttgcaaa tccgcgagaa gctcgccggt ttgcccggcg tcgcgctggt cagcctgccg
    13621 cccgagcagc ggaaagacgc cgaagccaag aaacggcttc ttgcggaagc cgatctcgtc
    13681 atcctgtgct tgccggatag cgccgccagg gagaccgtgg cgctgatcga cgggctcgcc
    13741 ggtccgcagc cgcgcatcat cgacgcttcg accgcccatc gcgtcgcgcc cggctggacc
    13801 tatggctttc ccgaactgac ggccggacag gccgcggcga tcgcggcggc caagcgcgtc
    13861 gccaatcccg gctgctacgc gaccggcgcg atcgcgctgc tccgcccgct cgtcgaggcc
    13921 ggcctgatcc cggcctctca tcgcgtgacg atcaatgcgg tgagcggcta tagcggcggc
    13981 ggcaaggcga tgatcgcggc ctatgaaagc ggctcggcgc cggctttcga gctttacggc
    14041 ctcgccggcg agcacaagca cctgccggag atcgtggcgc attccggcct ggcccggcgc
    14101 ccgattttcg taccttcggt cggcaatttc aagcagggca tgctggtcag cattccgtta
    14161 tggctcgacg aattgcccgg ccggccggac cacgccgcgc tcgccgcggc gctcgccaag
    14221 cactatgccg gctccgccta tgtgtcggtc gtcccgtgcg gcgatcagag ccacgcttcc
    14281 ggccggctcg atgcgctgtc gctcaacggc acgaacgaga tgcaattgca tattttcggc
    14341 gacccgacgc agcggcaagc cgttcttgtc gccaagctcg acaatctcgg caaaggcgcc
    14401 tcgggcgccg ccgtgcagaa cctcaagctg atgctgggac tcgaaaaggc ggcgcgggcg
    14461 gcgtaagctg tgcagcctct tcgtgatggc gagaaaaggc aaatctcgcg cgccgccgcc
    14521 gcgaatgcta tgctaggacc catgagcgtt cccgtgtcgc gcgccgccga gggcctcgcc
    14581 cgccgcgcct tcaccatcgc cgatctcgag cgaatggcgg aggtcggcct gatcgcgccg
    14641 gacgagcgtc tggagctgat cggcggagaa atcgttccga tgtcgcccaa ggggtcacgc
    14701 cacgagacga tcaaggcggc catcaatttt cactggggac aacgctgtcc ggccggtttc
    14761 gctttcatcc cggaaactgg ccttcggctc gacgagcgta cttatattga gccggacttc
    14821 atcgtcttcg agcgcaagcg ccggcttgcc gacatccggg ggccggacgt tcttctcgcc
    14881 gtcgaagtcg ccgattcgtc ccttcgctac gatctcgacc gcaagccgct ggtctatgcc
    14941 ggcttcggcg ttcgcgaact gtgggtgatc gacgccgagc gccacgccgt ccaccgccac
    15001 gccgagccgt cgccggtcgg ctataagcgc atcgaggaat atggcggcgc cgaccgcttg
    15061 acgccgctcc acgcgcccgc gccgttcgca ttcgcgctcg acgaattgga gccgatctga
    15121 ttggcctcag ccgcgccgcg ccagccgcca tgtggcgcgc aataggcctt ccgtgatcgc
    15181 ccccgcaaga taagcgaggg cgcagcagaa caaggcggcg gcgacgactt tgcccgtctc
    15241 gtcccccgac atgctgaacg cgccgctggt caggacgctc atcgtccagg tgaacggcat
    15301 agcgatcagc agcaaggtca aatcggcgag ccactggccg gcgttctgaa cgtagatgac
    15361 gtaaccggcg atgatcgcgg cgagataaag cgccgccaag gccgcgccga ggagcgacag
    15421 aaagggacgt cggcgcggcg cggcggcgtt gaaggtcaag cttctttcgt ccaggcgacg
    15481 aagcgctggc gctgctcgcc taagccctcg atgccgagcg cgaccgtatc gccggctttc
    15541 aggaagcgcg gcggcttcat gccgaggccg acgccgggcg gggtgccggt ggtgatcacg
    15601 tcgcccggca gcagaaccat gaaatgcgag atgtacgaca ccagcatctt gacgtcgaaa
    15661 atcatcgtcg aggtcgagcc gttctgcatt ctctggccat tgacgtcgag ccacatcgag
    15721 agcttctgca cgtccggtat ctcgtcgggc gtgaccagcc acggaccgag cggaccgaat
    15781 gtcgcgcagc ccttgccctt catccattgg ccgccgcgct cggcctggaa ttcgcgctcc
    15841 gacacgtcgt tgcacacgca aaagccggcg atatagtcga aagcttcatg gtcggcgaca
    15901 taggaggcgc ggcggccgat cacgaccgcg agctcgacct cccagtcggt tttgaccgag
    15961 gccttcggga tgacgacatc gtcgttcggg ccgacaatgc aggacggcgc cttgttgaaa
    16021 aggataggct ctttgggaat cgccgcgccg gtctcggcgg cgtgatcggc gtagttgagg
    16081 ccaacggcga tgaaattgcc aacctgcccg acgcaagcgc cgagccgcgg ctcgccgcgc
    16141 accgccggca ggctggccgg cttgagcttg gcgatcttgg cgaggctcct cggcgacaag
    16201 gcttcgccgg caagatcgtc aatgaccccg ctgagatcgc gcaatgcgcc ggcgccgtcg
    16261 atgaggcccg gtttttcctt gcccggcttg ccgtagcgga cgagtttcat tagcgcgttt
    16321 ctcccttgat cgcacatttg cgaaagcgct ctcggttagc tgattttcgc ctccgggaaa
    16381 acccgtcgcg cgagccgtct gacaaagaaa gcgtaacggg aaaatcagcc gctcggccgc
    16441 aagcaattgc gctggagaat ggcggtaaat tacgacccct ggcgtggcga atgacgcggt
    16501 cctaaacaac gttgaagaag cggccaatgt gaacggctcg acgccgcggt gaaatcggtt
    16561 gcttactttg ccgggataaa agcaagcctc caaaacggaa gcatctcgtt ggagaagatc
    16621 atgacatttc catttgttca cttgacttga tcgacgccgt cgccctgata tgccaatgaa
    16681 aatgaaagag acgcgacgaa tcattaagcg cgatcgccgc tcgcgcggtt tcgtattgct
    16741 ggatgaccct gctttatcgg ttgaggtcct ttcggtttaa atcgtccgtt gcggcagctt
    16801 tgttgcttct ttgcgagaga gacaatcgaa tatggctgat caggaaagaa tagcgctgtt
    16861 catcgacggc gcgaatcttt acgctacggc aaaatcgtta ggattcgaca tcgattacaa
    16921 gcgcctgttg cgcgaattcc agagccgggg ccgtttcgtg cgggctttct actacacggc
    16981 cctggtcgaa gatcaggaat attcttcgat ccggccgctt gtcgattggc tcgactataa
    17041 cggctattcc gtcgtgacca agccgaccaa ggaattcgtc gattcgaccg ggcggcgcaa
    17101 agtcaaagga aacatggaca tcgaattggc cgtcaacgcg atggagatgg cggcgcatat
    17161 cgaccacatg gtcctgttct ccggcgacgg cgattttcgc tccctggtcg aggcggtgca
    17221 gcgccgcggc gtccgcgtct cggtcgtgtc gaccgtccag acccagccgg ccatggtcgc
    17281 cgacgaattg cgccgccagg ccgacgaatt catcgacctc gccagcctcg ccagccggat
    17341 cggccgcgat ccaagcgacc ggccgccgcg ccatgccgac gcccgccgcg ggccgccgca
    17401 gcaggctggc ttggtcaatg atgatttcgt cgacggctga ggaggcgctc gcccccgcgc
    17461 ctcaatcgaa attgcccttc gacgtcagcc caccgcgcga ttgcccggcc tgtccgcggc
    17521 tcgccgaatt tcgcgccgcc aatcgcgccg cccatcccga ttggcgcaac gagccggtcg
    17581 catcctgggg cgcggcggat tcccggctgc tgatcgtcgg cctcgccccg ggtctgcgcg
    17641 gcgccaatcg gaccggccga ccgttcaccg gcgatttcgc cggcgacctc ctctacgaga
    17701 cgctgctcgc gttcggcttc gcccggggcg tctacgaccg ccggccggac gatgggctgg
    17761 agctcgtcga ttgcgcgatc gtcaacgccg tgcgctgcgt tccgccgcaa aacaagccga
    17821 cgccgcagga aatcgcgacc tgccgcgcct atctcgccgg cgcgatcggg gcgctgaaaa
    17881 atctcgaagc catcgtcgcg ctgggacgga tcgcgcatga cagcgccttg cgggcgatgg
    17941 gtctgcctct tgcaggcttt cccttcgccc atggcgccgc ccatcaattg cccaacggga
    18001 ccgcccttta cgacagctat cactgctccc gctacaacac gaataccggc gtcctgacgc
    18061 cgaaaatgtt cgccgaggtc tttgccgccg tccggcggcg gcttggcgcg cctggccgcg
    18121 cgggcttgcc ttgaccgatc ggcgggtgta ggagcccgcc aaaccccaga ttgccggact
    18181 tttcttcggc cgagctcttt taggagttgc cgccatggcg cgcgtcactg tcgaggattg
    18241 catcgacaaa gtcgaaaacc gtttcgatct cgttctgatg gcgagtcatc gcgcccgcat
    18301 gctgtcggct ggcgcgcaga tcaccgtacc gcgcgacaac gacaagaacc cagtcgtcgc
    18361 cttgcgcgag atcgccgacc agcggctgtc gccggaagat ctgaaggaag atctcattca
    18421 ctcgctgcaa aagaacgtcg aggtcgacga gcccgaggcc gagacggcgc cgccgctggt
    18481 cagcgccgac attgcgccgg gcgaagcggg cgacgcgcag ttcgaccgca tgaccgaaga
    18541 agatctgttg cgcggcctcg aagggctggc gccgccgccc gagccggaag aggaagccga
    18601 gtaggcggcc gaagccccgc tctgacgcaa ccgtcttcgt tcaattgata tttcgcgccg
    18661 cagcggatgc ggcgcggcta tttccacaaa gtttcttgac gcgccgcaga tttcgctatg
    18721 tgggcgccgg cgttttgcta tcctctgcat tgagagaatc acttttgtga accgttgtgc
    18781 aaaggcgcgg cggagcggcg ggtcagcgct taacgttttc gacctgcggc ggacaggcgc
    18841 ggcgaaagcc gaatcgcgaa gaggggcgcc ggccggcaag gacgcatgac cttgttccgg
    18901 cgccaggggc ggagcgattg ttcgccggcg acgcggcgtt tcgagtgggg gccgacattt
    18961 gagcgagaac gaatcaaagc tgcgtctcga catccgcgaa caagccgcag acgcggtcga
    19021 gccgccggtc ggcctggtcg aagtggccgg ctatgtgaaa tggttcgatg tcggcaaagg
    19081 ctacgggttc atcgtcccag acggcggcaa gccggacgtg ctcatccatg tcacggtgct
    19141 gcgccgcgac ggattccaga ccgtgcatga gggcgcccgc gtcgtcgccg aggcggtgca
    19201 gcgcgaaaag ggcttgcagg tcttccgggt gatctcggtc gagcccggcg acggctcgca
    19261 tccatcacaa ttgccgccgg cccgcaccca tgtccaggtg acgccgacca gcgggttcga
    19321 gatcgtcatc gtcaaatggt tcaaccggg
//
LOCUS       DATGJW010000200         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_659208, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000200 DATGJW010000000
VERSION     DATGJW010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_659208"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..907
                     /locus_tag="VKU03_06240"
     CDS             329..907
                     /locus_tag="VKU03_06240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011084225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70899.1"
                     /translation="MRADIIPFIFALGLALAAPIAVAPLPAFAQAQQPEAGTPIKQIA
                     LTQNQIDSYLSAKKEIDAVLEKLPEDSQGQAPDPKTTAQLDAIAKKNGLANYDEYESI
                     EDNIGLVIDGMDPQTKKYIGAEAVIKQEIAQLKAEKSMAPKDKKEALEQLNAALKSAT
                     PVQNPGNIELVTKNFDKINAAMPQDQEQPQKP"
     gene            complement(980..2185)
                     /locus_tag="VKU03_06245"
     CDS             complement(980..2185)
                     /locus_tag="VKU03_06245"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008833823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ70900.1"
                     /translation="MIEKDIVALAAWIAEAGLAGKTETALVQGFCARAVASGLRLARA
                     VLLIDTLHPIYEGRAIRWELEKSEATLVEYGSSTEGEAAARWRASPFYRLVSSGETLL
                     RRRITEDSIAEFPGLADSRAAGITDYVAMVNPFAADGAIGEMDCFYSSWMTNRSDGFS
                     NAEIAAIERLTPFLGLAIKSASLGRIAQTLVETYLGRDAGRRVLSGRIARGVADRIET
                     VLWFSDLRNYTRLSDASPPELIIPLLNDYADAIISAIHDHDGDVLKLIGDGVLAIFPA
                     TERRQACAAALDAARHAQKSVRALNASRSGQGLPATDMYLGLHLGEVYYGNIGSRERL
                     DFTVIGPAVNEVSRIAAMCRSVDQPVLVSAAFAAAAAAEPSPFVSVGRYALRGVARPQ
                     ELFTLEAEA"
     gene            2392..2895
                     /locus_tag="VKU03_06250"
     CDS             2392..2895
                     /locus_tag="VKU03_06250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007824116.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-degrading domain-containing protein"
                     /protein_id="HLJ70901.1"
                     /translation="MDKAALEEDIRRLAEQERLLVFARFGPQEAWTLGALLRDMALAG
                     AAPVAIDISLRDRTLFHCAMPGSTADNAEWIRRKRNTVLRLWRSSYAVGLSLDLAGKS
                     QESAFALPLADYAVHGGAFPIALEALGCVGAVTVSGLPQREDHRLVADALAQFLNVDL
                     DKSRLSG"
     gene            3080..3481
                     /locus_tag="VKU03_06255"
     CDS             3080..3481
                     /locus_tag="VKU03_06255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HLJ70902.1"
                     /translation="MGDPSHVELAAKIISAFVANNSLPRNELAALIGEIHAAFARLSI
                     GAPAEPEVLTPAVPAKKSVNKDHLVCLEDGQKFKSLKRHLRTQHDLSPEQYRAKWGLP
                     SDYPMVAPAYAVTRSSLAKKMGLGQRRGRRK"
     gene            complement(3648..4403)
                     /locus_tag="VKU03_06260"
     CDS             complement(3648..4403)
                     /locus_tag="VKU03_06260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002713986.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLJ70903.1"
                     /translation="MTLEIVQAGADARANNLLRALRPSDWAILSPRLEEWSAPTGAIL
                     HEPGDTVRYAFFPRGSSLISYLVVLGDGRAIETALVGREGAVGGIVSKGRLPAYARAE
                     VHLGGLFYRIELQHLEDAKSRSPTLAQLFARYADCLMAQIFQSVACNASHSIEQRMAK
                     WFLAAIDRTGAVDMGLTQEQLAAMLGVGRSYLSRIIHEFRERAIIDTRRKRIVVRDVD
                     GLRALACECNAAVCRHFEEVLEGVYPAAGLAAE"
BASE COUNT          888 a         1562 c         1507 g          766 t
ORIGIN      
        1 cggcgtgctc ggcaacggcc tgacgcaaat gaacgtcgac agctatgtcc gcgacatgct
       61 gaccggatcg atcgtcatcg tcgccgtggc ggcgagcagc ctgagcaagc tgcgcaggcg
      121 atagcgcgcg accgtcaatc cactcgtgag acgcaatctt gtgaatgcat ttgcttcgtc
      181 gcaaaccagc tacgatctgg cgcgagcgga aacccgctcc gcaaatcggc gcgccctgtg
      241 tgtagccgac cgctcgctgg cgcatcgatc aagggcgcgt atactctccc gccgttcgaa
      301 tcgaacccgc ttgcttgaag gagaatccat gcgcgccgac atcatcccct tcatcttcgc
      361 tctcggcctc gcgcttgccg cgcctattgc tgtcgcgccc cttcccgctt tcgcgcaggc
      421 gcagcagccg gaggccggga cgccgatcaa gcagatcgcg ctgacccaga atcagatcga
      481 ctcctacctc tcggcgaaga aggagatcga cgccgtgctc gaaaaactgc cggaggattc
      541 gcaaggccag gcgcccgacc cgaaaacgac ggcgcagctc gacgccatcg ccaagaagaa
      601 cggcctcgcc aattacgacg aatatgaatc gatcgaggac aatatcggct tggtcatcga
      661 tggcatggac ccgcagacca agaaatatat cggcgcagaa gcggtgatca agcaggagat
      721 tgcgcagctc aaggcggaaa agagcatggc gccgaaggac aagaaggagg cgctggagca
      781 attgaacgcc gccttgaaat ccgcgacgcc ggtacaaaat cccggcaata tcgagctcgt
      841 gaccaagaat ttcgacaaga tcaacgccgc catgccgcag gaccaggagc agccgcagaa
      901 gccgtgacgg gacgtccgcc gccgtcattc ccgcgaaaga gggaaatccg gagcggtctg
      961 cgcgctcggc ggcggcgcct cacgcctccg cctcaagcgt gaacagctct tgcggccgcg
     1021 ccacgccgcg cagcgcgtag cggccgaccg acacgaacgg gctcggctcg gccgccgcgg
     1081 cggcggcgaa ggccgcagac acgagcacgg gctggtcgac gctccggcac atcgccgcga
     1141 tccggctgac ctcgttgacc gccggaccga tcaccgtgaa atcgagccgc tcgcggctgc
     1201 cgatattgcc gtaatagact tcgccgagat gcaggccgag atacatgtcg gtcgccggca
     1261 ggccctggcc gcttcggctg gcgttcagcg cccggaccga cttttgcgcg tgccgcgcgg
     1321 catcgagcgc cgcggcgcag gcttggcggc gctccgtggc cgggaagatc gccagcacgc
     1381 cgtcgccgat cagcttcaac acgtcgccgt catgatcgtg gatggccgag atgatcgcat
     1441 cggcatagtc gttgagcagc ggaatgatca gttccggcgg cgaagcgtcg gaaaggcggg
     1501 tgtagttgcg cagatcgctg aaccacagca cggtctcaat ccgatcggcg acgccgcgcg
     1561 cgatgcggcc gctgagaacg cgccggcccg cgtcgcggcc gagataggtt tcgaccagcg
     1621 tctgcgcgat ccggccgagc gaagcggatt tgatcgccaa gccgaggaac ggcgtcagcc
     1681 gctcgatggc cgcgatctcc gcgttcgaaa agccgtcgga ccggttggtc atccatgacg
     1741 agtaaaagca atccatctcg ccgatcgcgc cgtccgccgc gaaaggattg accatcgcta
     1801 cgtaatccgt gatcccggcg gcgcgggaat cggcgaggcc cggaaactcg gcgatgctgt
     1861 cctcagtgat gcgccggcgc agcaaagttt cgccgctgct caccagccgg tagaaggggc
     1921 tggcgcgcca gcgcgccgcc gcctcgcctt ccgtgctcga tccatattcg accagcgtcg
     1981 cctcgctttt ttccagctcc cagcgaatcg cccgcccttc atagatggga tgcagcgtgt
     2041 cgatcagcaa aaccgcgcgg gcgagccgca gcccgcttgc cacggcgcgg gcgcaaaagc
     2101 cttggaccag agcggtttcg gtcttgccgg caagtccggc ctccgcgatc cacgcggcga
     2161 gcgcgacgat gtctttctcg atcatgaggc gatcataatc ctcccccgcg ctgtggggga
     2221 gggggaccat gcgaagcatg gtggaggggg tacggcggtt gaggattatc ctgaaagacc
     2281 gcgccccctc caccgcgctg acgcgcggtc cccctccccc gcctcgcggg ggaggattgg
     2341 cgccctcgcg caaaacagta gagtcattct cccgatttaa ggatagagac catggacaaa
     2401 gcggctctcg aagaagacat tcgccgtctc gccgagcagg aacgcttgct cgtcttcgcc
     2461 cgtttcgggc cgcaagaggc ctggacattg ggcgcgcttt tgcgcgacat ggccctcgcg
     2521 ggcgcggcgc cggtcgcgat cgacatttcg ctgcgcgacc gtactctctt tcattgcgcc
     2581 atgcccggca gcacggccga caatgccgaa tggatcaggc gcaagcgcaa caccgtgctc
     2641 aggctgtggc gctcgtccta cgcggtcggc ctctcgctgg atttggccgg aaaaagccag
     2701 gagagcgcgt tcgccttgcc gctcgccgac tatgccgtcc atggcggcgc cttcccgatc
     2761 gcgctcgaag ccttgggctg cgtcggcgcg gtgacggtat ccggtctgcc gcagcgcgag
     2821 gaccataggc tggtcgccga tgcgctggcg caattcctga atgtcgattt ggacaaaagc
     2881 cggctcagcg gctagccgcg cccgcggcca agccggaagc ggcgaaacgg ttcttgcgca
     2941 gattcttttg aataagccag acgccaggcg gcttttccgt tgcggcgcgc gcgctcttcg
     3001 gcgaggctcc gccgcgaatc ggcggataca taaaaagatg tggcgcaaag ggaggtcgca
     3061 caagcagcag gtcgcatcaa tgggggatcc aagtcacgtc gagctcgccg caaaaatcat
     3121 ctcggctttt gtggcgaaca attccttgcc gagaaacgag ctggccgccc tgatcggcga
     3181 gatccacgcg gctttcgcgc gactttcgat tggcgcgccc gccgagcccg aagtcctgac
     3241 gcccgccgtt ccggccaaga aatcggtcaa caaggaccat ctcgtctgtc tcgaagacgg
     3301 acagaagttc aagtcgctga aacggcactt gcgcacccag cacgatttgt cgccggagca
     3361 gtatcgcgcc aaatggggcc tgccctccga ctacccgatg gtcgcgccgg cctacgccgt
     3421 cacgcgatcg agcctggcca agaaaatggg actcggccaa cgccgcggcc ggcggaagta
     3481 gcgtcgcccg gcggccgcgc ttactgtcat ttgcgaggag cgaagcggca aagcagtcca
     3541 ctcgtagaga tctgcgctcc atgagtggat tgcttcgctt cgctcgcaat gacggcgcga
     3601 ctgacttggc tcgccggcct ttgcccgcgc gaagccggcg tcggccgcta ctccgcggcg
     3661 aggccggccg ccggatagac gccttccagg acttcctcga aatgccggca gaccgcggcg
     3721 ttgcattcgc aggcgagcgc ccgtaggccg tcgacatcgc gcacgacgat ccgcttgcgc
     3781 cgcgtgtcga tgatcgcgcg ctcccggaac tcatggatga tgcggctcaa atagctgcgc
     3841 ccgacgccga gcatcgcggc gagttgttcc tgggtaaggc ccatatcgac ggccccggta
     3901 cggtcgatcg cggcaaggaa ccatttggcc atgcgctgct cgatcgagtg cgacgcattg
     3961 cacgcgaccg attgaaaaat ttgcgccatc aggcaatcgg catagcgcgc aaagagctgg
     4021 gccagagtcg gcgatcgcga tttcgcatcc tccagatgct ggagctcgat gcgatagaac
     4081 agaccgccga gatgcacttc ggcgcgcgca taggcgggca gccggccctt gctgacgatc
     4141 ccgccgaccg cgccttcgcg cccgacgaga gccgtctcga tcgcccgacc gtcgccgagc
     4201 acgaccaggt aggagatcag gctcgatccg cgcgggaaga aggcgtagcg caccgtgtcc
     4261 ccgggctcat gcaggatggc gccggtcggc gccgaccatt cttcgagccg cgggctgaga
     4321 atcgcccaat cggatggccg cagcgcgcgc agcaggttgt tggcgcgggc gtccgctccc
     4381 gcttgcacta tttccagagt caagatggag ccccgccgac cgcgtctcct ccttctattc
     4441 gccgccccta gaaatgtcca gtagtggaca gaccggcgag tcgtttgccg ctacaaagct
     4501 ttgcctggac gtcgccgatc ctcgccaatt ccacaattcg gcccgggaga gggaagagaa
     4561 tgccgaggac gcaaaagccg gccggcgggc cctgcctgcg ccgaaatcag ccgaacagcc
     4621 aggcgtgagg atgatcgtca gcaagtcgcc aggctccgat agctttccgc gtttgcggct
     4681 ttacgtcgcc ggcgacgcgc cgggcgcccg gcgggcgctg ttc
//
LOCUS       DATGJW010000201         3147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_762077, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000201 DATGJW010000000
VERSION     DATGJW010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3147
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762077"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..1629)
                     /locus_tag="VKU03_06265"
     CDS             complement(16..1629)
                     /locus_tag="VKU03_06265"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase"
                     /protein_id="HLJ70904.1"
                     /translation="MRQTGVYNAAHGADKFGFKDLSAVYWSLRAPHLYGEALARGEAA
                     IAQGGALVAETGVHTGRSPKDKFILRDAATEKSIWWDNNNAISREHFDTLLADFIAHA
                     KGRELFAQDLYAGADPSFRVKARVFTELAWHSLFIRNLLIRPEAGELASFVPDFTIVD
                     LPTFKADPKRHGCRSETVIACDFSRRIVLIGGTSYAGEMKKAVFTYLNFMLPDHDVMP
                     MHCSANSGGRNEVALFFGLSGTGKTTLSADPTRMLIGDDEHGWSKHGVFNFEGGCYAK
                     AIRLSREAEPDIYATTERFGTVMENVVLDPVTHEPDFDDDSKTENTRIAYPLDFVANA
                     SRTGRDGRPRNIVMLTCDAFGVLPPIAKLDPSQAMYHFLSGYTAKVAGTEKGVTEPQA
                     TFSTCFGAPFMPRHPSVYGNLLRDLIEKHHVDCWLVNTGWTGGKHGVGRRMPIRVTRR
                     LLAAALDGSLNRANFRSDPYFGLAVPTRVPGVEPHILEPIKTWASKAEFAETAKKLIA
                     MFRENFVKFERHVDEEVRSAGPTPRIAAV"
     gene            complement(1706..>3147)
                     /gene="xylB"
                     /locus_tag="VKU03_06270"
     CDS             complement(1706..>3147)
                     /gene="xylB"
                     /locus_tag="VKU03_06270"
                     /EC_number="2.7.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004856 - xylulokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0005997 - xylulose metabolic process
                     [Evidence IEA];
                     GO_process: GO:0042732 - D-xylose metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="xylulokinase"
                     /protein_id="HLJ70905.1"
                     /translation="AALVDEEQNVLAAASEAYRPANPHPLWSEQNPDDWLRAMFAALA
                     TLGRENRQAMARVSAIGFSGQMHSAVVLDGADKPLRPAILHNDTRAFAEARRLAENHR
                     ELAPIVGVKPMAGFTAPKLMWLAAHEPAIFKKIRCVLLPKDYLRLALTGQKATDMSDA
                     AGTWWFDEAARQWSHEALAASGVDPALAPPLVEGSAPAGVLRDDIADRFGLKRGAIVA
                     AGGGDAAVGAIGLGAVKAGAAFISLGTATQLIVAQDRYAATPPERLVHGFAHALPNRW
                     YRMAAMLNGAGALAFLGRLFSTEPAELEAEAARDYRGPGAITALPYLSGERTPHDDPL
                     ARGVFFGLSPSTSRGELSRAMMEGVALSLRDAKDAIESSQGKIDAVGLTGGGAKSALW
                     TQMIAAALDLPVVRYQGGDAGPAFGAARLARLAATGEMPEDVCIAPPVLDEIKADAAL
                     IEAFAARIDRFRSLYRALAPEFAAARYRR"
BASE COUNT          574 a         1022 c         1020 g          531 t
ORIGIN      
        1 gcggggtgaa cggcctcaca ccgccgcgat acgcggcgtc ggcccggcgc tgcgcacctc
       61 ctcgtcgaca tggcgctcga acttgacgaa attctcgcgg aacatggcga taagcttctt
      121 cgcggtctcg gcgaattccg ccttgctcgc ccaagtcttg atcggctcga gaatatgcgg
      181 ctcgacgccg ggaacgcggg tcggcacggc gaggccgaaa taaggatcgg agcggaaatt
      241 ggcgcgattg agcgagccgt cgagcgcggc ggcgagcaac ctgcgcgtca cccggatcgg
      301 catgcgccgg ccgacgccgt gttttccgcc ggtccagccg gtattgacca gccagcaatc
      361 gacatggtgc ttctcgatga gatcgcgcaa gagattgcca taaaccgacg gatggcgcgg
      421 catgaaaggc gcgccgaaac aagtcgagaa cgtcgcctgc ggctcggtca cgcctttctc
      481 ggtgccggcg accttcgcgg tatagcccga caggaaatgg tacatcgcct ggctcggatc
      541 gagcttggcg atcggcggca acacgccgaa cgcgtcgcac gtcagcatga cgatattgcg
      601 cggccgcccg tcgcgtccgg tccgcgaagc attggcgacg aaatcgaggg gataggcgat
      661 gcgggtattt tcggttttcg aatcatcgtc gaaatccggc tcatgcgtca ccggatcgag
      721 aaccacattc tccatcaccg tgccgaagcg ctcggtagtc gcgtaaatgt cgggctcggc
      781 ctcgcgggag aggcggatcg ccttggcgta gcagccgccc tcgaaattga agacgccgtg
      841 tttgctccag ccatgctcgt cgtcgccgat cagcatacgc gtcggatcgg ctgaaagcgt
      901 cgtcttgccg gtgccggaaa gcccgaagaa gagcgcgact tcgttgcggc cgccggaatt
      961 ggccgagcaa tgcatcggca tcacgtcatg atccggcaac atgaagttca gataggtgaa
     1021 gaccgccttt ttcatctcgc cggcgtagct ggtgccgccg atcagtacga tgcggcggct
     1081 gaagtcgcag gcgatcaccg tctcgctgcg gcaaccatgg cgtttggggt ccgccttgaa
     1141 ggtgggcaga tcgacgatgg tgaagtccgg tacgaaagag gcgagttcgc cggcttcggg
     1201 ccggatcagc agattgcgaa tgaacaggga atgccaggca agttcggtga agacgcgcgc
     1261 cttgacgcgg aaggacggat cggcgccggc gtagaggtcc tgcgcgaata gttcgcggcc
     1321 cttggcgtga gcgatgaaat cggcgagcaa tgtatcgaaa tgctcgcgcg agatggcgtt
     1381 gttattatcc caccagatgg atttttcggt cgccgcgtcg cgcaggatga acttgtcttt
     1441 tggactgcgg ccggtgtgaa cccctgtctc cgccacaagc gcgccgcctt gcgcgatcgc
     1501 cgcctcgccg cgcgccagcg cctcgccata gagatggggc gcgcgcaggc tccaatatac
     1561 cgccgacaaa tccttgaacc cgaatttatc ggcgccgtgg gccgcgttgt acacccctgt
     1621 ctgtcgcatc cgaacttcct ccctagcaac gcgccttaaa ataacattag gggagcgcgg
     1681 atgatatcgc ccgcccctgg gaatattaac gcctataacg cgcggcggcg aattccggcg
     1741 ccagcgcccg gtaaagagat cgaaagcggt cgattcgcgc ggcgaaagcc tcgataagcg
     1801 cggcatcggc tttgatttcg tccaaaaccg gcggcgcgat gcagacgtcc tccggcattt
     1861 ccccggtcgc ggcgagacgg gcgagccgcg ccgcgccgaa agccgggcca gcgtcgccgc
     1921 cctgatagcg gacgaccggc agatccaggg ccgccgcgat catttgcgtc caaagcgcgc
     1981 ttttggcccc gccgccggtc agtccaacag catcgatctt tccttggctg gattcgatcg
     2041 cgtccttggc gtcccttaag cttaacgcca cgccctccat catcgcccgg gaaagctccc
     2101 cgcgactcgt cgaaggcgaa aggccaaaaa atacgccgcg cgccagcgga tcgtcgtgcg
     2161 gcgtccgctc gccggaaaga tagggcaagg cggtgatcgc gccggggccg cgataatccc
     2221 gggctgcctc ggcttcgagc tccgccggct ccgtgctgaa aagccgcccc agaaaggcga
     2281 gcgctccggc gccattgagc atcgccgcca tccgatacca gcggttcggc agcgcatgcg
     2341 cgaaaccatg caccagccgt tcgggcggcg tcgcggcgta gcggtcctgc gcgacgatca
     2401 attgcgtcgc cgtgcccaag gaaataaagg ccgcccccgc tttgacggcg ccaaggccga
     2461 tcgccccgac cgcggcgtcg ccgccgcccg ccgcaacgat cgcgccgcgc ttcaggccga
     2521 aacggtcggc aatgtcgtcg cgcaagacgc cggccggcgc cgagccttcg acaagcggcg
     2581 gcgccagcgc gggatcgacc ccgctcgccg cgagcgcttc gtgggaccat tgacgcgccg
     2641 cttcatcgaa ccaccaggtc ccggcggcgt cgctcatgtc ggtcgccttt tgccccgtga
     2701 gggcgaggcg cagatagtcc ttcggcagca ggacgcagcg gatttttttg aagatcgccg
     2761 gctcatgcgc ggcgagccac atcagcttgg gcgccgtgaa ccccgccatc ggcttgacgc
     2821 cgacgatggg cgcgagctct cgatgattct cggcgagccg ccgcgcttcg gcgaaagcgc
     2881 gcgtgtcatt gtgcaggatc gccggacgca gcggcttgtc cgctccatcc agcacgacgg
     2941 cgctatgcat ctggccggaa aagccgatcg ccgagacgcg cgccatcgcc tggcggtttt
     3001 cgcgcccaag cgtcgccagc gccgcgaaca tggcgcgaag ccaatcgtca gggttttgtt
     3061 ccgaccaaag cggatgagga ttggcgggcc gataggcctc ggaggcggcg gcgagaacat
     3121 tctgctcctc atcgaccagc gccgcct
//
LOCUS       DATGJW010000202         3705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_762148, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000202 DATGJW010000000
VERSION     DATGJW010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3705
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_762148"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..930)
                     /locus_tag="VKU03_06275"
     CDS             complement(262..930)
                     /locus_tag="VKU03_06275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HLJ70906.1"
                     /translation="MKLNRDYKAPAELPRTIPVFPLAGALLLPRRPLPLNIFEPRYLA
                     MVDDALSGERLIGVIQPSAGEDAVEPRPELYPVGCAGRITQFAETGDGRCFVTLTGIA
                     RFRIVDEPPNVTPYRLAQVDFADFAADFREGEGESDVDRQALLAALREFAEANRFKVD
                     WDDIEKASNEALVNGLSMLSPYGPKEKQALLEAETLQARAEMLMAITHMELARATDAP
                     SRVH"
     gene            complement(960..1853)
                     /gene="trxA"
                     /locus_tag="VKU03_06280"
     CDS             complement(960..1853)
                     /gene="trxA"
                     /locus_tag="VKU03_06280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin"
                     /protein_id="HLJ70907.1"
                     /translation="MAAEAANSGAVKDVTTASFREDVLTASTKQPVLVDFWAPWCGPC
                     KQLAPVLEKAVAAAGGKVKLVKMNIDEHPQVAGQLGIQSIPAVIAFEKGQPIDGFMGA
                     LPETQVRGFIERLVGPLTGGAEELIGQAEAALAAGDASGAAALYARVLTEDEGNAKAI
                     AGLAKIHIDNGELEQAKGVLSLAPPPPPGRDADPLISAAKAALDLAEQAASLGDAAPL
                     EAALAADPANNQARFDLALALNARGEREKAADHLLEIVKRDRQWNDDAARKQLVQFFE
                     AWGLMDPAAVAARRKLSALLF"
     gene            complement(1936..2445)
                     /locus_tag="VKU03_06285"
     CDS             complement(1936..2445)
                     /locus_tag="VKU03_06285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017989752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HLJ70908.1"
                     /translation="MDQPATPEDLFARLDALGVEHDTIEHPPLFTVEDSQSLRGEIPG
                     GHTKNLFVKDKKGRLFLLVLGEDAAVDLKRVHEKIGGQGRVSFGSAELLEEVWGVKPG
                     AVTPFGAINDRSGRVTVVLDAALLAESRLNFHPLVNTRTTGLASGDLVRFLRATGHEP
                     LIVALAEKG"
     gene            complement(2498..3649)
                     /locus_tag="VKU03_06290"
     CDS             complement(2498..3649)
                     /locus_tag="VKU03_06290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel/cobalt transporter"
                     /protein_id="HLJ70909.1"
                     /translation="MSAGRPMRGAMKTPSPASGRGGAWRSSILPALKRRPLTLLLAAL
                     VLAFAAHPALAQFAHRPFEVAGGEGGGRAAGITGWILAEQARLVHAMTGLMKALRESR
                     SAFRGLAALSFLYGVVHAAGPGHGKALIASYMLANERALRRGIVLAFFAALLQALVAI
                     ALIGVAALVFRATAIQMNDAARLLEMASYAAIAAIGLWLVWSKGRALAAALRGLLAQR
                     AVLAGGALYAEAPWRPVLALESLGRFRAQSPTDAAMPADDCGHVHAPDPRQLGDGFSW
                     RGAAATVFAAGARPCSGAILVLVFALAQNLFLAGIGATLAMALGTAITTGALAMLAVF
                     AKDIALRFARRESSLGGLIARLFEFAAALAVLAFGVLLLLGFGASLGGA"
BASE COUNT          619 a         1248 c         1277 g          561 t
ORIGIN      
        1 cacacgggtg ctcgatctga tggggcccgg attcgaccct caaatgttcc gcgaactggc
       61 ccgggtcacc gccgacggca agatcgatcc ccagcaggtc ttgcccattc tcacacgcta
      121 cggcgtccgc ccgaccgtct ccggcggatg acaaaaggcg cgcggggcag gcgccgccaa
      181 tcctatttaa gcaatcggct cgccatgaag gccacgatcg ctgacgcctt tcgtcctgga
      241 cttgattggg gaggcgcccg gctaatgcac ccgcgacggc gcgtccgtcg cgcgggcgag
      301 ctccatatgc gtgatcgcca tcagcatttc cgcgcgcgcc tgcagcgtct ccgcttcgag
      361 cagcgcctgc ttttcctttg gcccgtaggg gctcagcatt gaaagaccgt tgaccagcgc
      421 ctcgttcgag gctttctcga tatcgtccca gtcgaccttg aagcgattgg cttcggcgaa
      481 ttcgcgcaag gcggcgagga gcgcttgccg gtccacgtcg ctttcgcctt cgccttcgcg
      541 aaaatcggcg gcgaagtcgg cgaaatcgac ctgggccagc cgataaggcg tgacgttggg
      601 cggctcgtcg acgatgcgga aacgggcgat tccggtcagg gtgacgaagc aacgtccgtc
      661 gccggtctcg gcgaattgcg tgatgcggcc ggcgcagccg accgggtaga gctcggggcg
      721 cggctcgact gcgtcctcgc cggcgctcgg ctggatgacg ccgatcaagc gctcgccgct
      781 cagcgcgtcg tcgaccatgg cgagatagcg cggctcgaat atgttgagcg gcaagggccg
      841 gcgcggcagc agcagcgctc cggccaacgg aaagacgggg atcgtccgcg gcagctcggc
      901 tggggctttg tagtcgcggt taagcttcat tctgacgcga tctcgagagc ctgcgaaatc
      961 tagaacaaaa gggcggacaa tttgcggcgc gccgccaccg ccgcgggatc catcaagccc
     1021 caggcttcga aaaactgcac caattgcttg cgcgccgcgt cgtcgttcca ttgacggtcg
     1081 cgcttgacga tttcgagcag atgatcggcg gccttttcgc gctcgccgcg cgcattgaga
     1141 gcgagggcga gatcgaaccg cgcctgattg ttggccggat cggcggcgag cgctgcttcg
     1201 agcggcgcgg cgtcgccgag cgaggcggcc tgctcggcga gatccagcgc ggccttggcc
     1261 gccgagatca acggatcggc gtcgcggccg ggcggaggag gcggggcgag cgagagaacg
     1321 cccttggcct gttcgagctc gccattgtcg atgtgaatct tggcgaggcc ggcgatcgcc
     1381 ttggcgttgc cctcgtcctc ggtcagcacg cgggcataga gcgcggcggc cccggacgcg
     1441 tcgccggcgg cgagcgccgc ctcggcctgg ccgatcaatt cctcggcgcc gccggtcagc
     1501 ggcccgacga ggcgctcgat gaagccgcgc acctgggttt ccggcaaggc gcccatgaac
     1561 ccgtcgatcg gctggccttt ctcgaaggcg atcaccgccg ggatcgattg gatgccgagc
     1621 tggccggcga cttgcggatg ctcgtcgata ttcatcttga cgagcttgac cttaccgccg
     1681 gcggccgcga ccgccttttc cagaacgggc gccaattgct tgcaggggcc gcaccagggc
     1741 gcccagaaat cgaccagaac cggctgcttg gtcgaagctg tgagcacgtc ctcgcgaaat
     1801 gaggccgtgg tcacgtcctt gaccgcgccg ctattcgccg cttccgccgc catcctttgc
     1861 tccaattcaa aaattgtctc gcgctcgcgc ccaagatggg gccagcgcgg ccttttcgca
     1921 atattgcgcc gccgatcagc ccttttcggc gagggcgacg atcagcggct cgtggccggt
     1981 cgcgcgcaga aacctgacga gatcgccgct ggcgaggccg gtggtgcgcg tattgaccag
     2041 cggatggaaa ttgaggcggc tttccgccag cagcgcggcg tcgagcacca ccgtgacccg
     2101 gccgctgcga tcgttgatcg cgccgaacgg cgtcaccgcg ccgggcttga cgccccagac
     2161 ctcttcgagc agttcggccg agccgaacga gacgcggccc tgcccgccga tcttctcgtg
     2221 gacgcgcttg agatcgacgg ccgcgtcctc gccgagcacc agcaggaaca gccggccttt
     2281 cttgtccttg acgaaaaggt tcttggtatg gccgcctgga atctcgccgc gcagactttg
     2341 cgaatcctcg accgtgaaga gcggcggatg ctcgatcgtg tcgtgctcga cgccgagcgc
     2401 gtcgagacgg gcgaacaggt cttcaggcgt tgcgggctgg tccatgcggg ccgggcttta
     2461 gccgaggcgg cgaagagcgg caagacagtg gcgcgcgcta cgccccgccg aggctcgcgc
     2521 cgaagccgag caaaagcagc acgccaaagg caaggaccgc caacgcggcg gcgaattcga
     2581 agaggcgggc gatcaggccg ccgagtgagg attcccgcct ggcgaaacgc aaggcgatat
     2641 ccttggcgaa aaccgccagc atggccagcg cgccggtggt gatcgccgtg ccgagcgcca
     2701 tcgccaaggt cgccccgatt ccggcgagga acaggttctg cgccagcgcg aagaccagga
     2761 cgagaatcgc gcccgagcag ggccgcgcgc cggcggcgaa aaccgtcgcc gccgcgccgc
     2821 gccaggaaaa gccgtcgccc aattggcgcg gatcgggcgc gtggacatgg ccgcaatcgt
     2881 cggccggcat cgccgcgtct gtcggggatt gagcgcgaaa ccgacccagc gattccagcg
     2941 ccaggaccgg ccgccagggc gcctccgcat agagcgcgcc gccggcgagg acggcgcgct
     3001 gcgcgagcag gccgcgcagc gccgcggcca gcgcccgccc cttgctccag accagccaca
     3061 ggccgatcgc ggcgatggcg gcgtagctcg ccatttcgag gagccgcgcc gcgtcgttca
     3121 tttgtatcgc ggtcgcccgg aagacgaggg cggcgacgcc gatcagcgca atcgcgacca
     3181 gggcttgcaa caaggcggcg aaaaaggcga ggacgattcc gcgccgcagc gcgcgctcgt
     3241 tggcgagcat ataggaggcg atcagcgcct tgccgtggcc gggtcccgcc gcatgcacga
     3301 cgccgtagag aaagctcaaa gccgcgaggc cccggaacgc cgaacggctc tcgcgcagcg
     3361 ctttcatcag gccggtcatc gcgtgaacca gccgcgcctg ctcggcgaga atccagccgg
     3421 tgatccccgc cgcccggccg ccgccctcgc cgccggcgac ctcgaacggc cgatgcgcga
     3481 attgcgccaa ggccgggtgt gcggcgaagg cgagaacgag agcggcgaga agcagcgtca
     3541 gcggccgccg cttgagcgcg ggcagaattg acgagcgcca cgcgccccct ctcccgcttg
     3601 cgggagaggg ggttttcatc gcgccgcgca tcggacgtcc cgcgctcatg ggcaagcgat
     3661 gatcgcgcgg ctcgccaatt tgaagccgaa gccggcgccg ggcga
//
LOCUS       DATGJW010000203        10830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1282292, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000203 DATGJW010000000
VERSION     DATGJW010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10830
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282292"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..780
                     /locus_tag="VKU03_06295"
     CDS             <1..780
                     /locus_tag="VKU03_06295"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70910.1"
                     /translation="SQEIEAAVAAGARRLLSFGVAGALIPQLQPGDLILAHGVRDGRR
                     SLTCDKDWRSAIGRRLQRPGSPRAAASAASEAALKSALFRFGRQSGWRPVLDAAERYP
                     IAEIAGADAPVADEIGKSALFAATGAVAVDMESVLVARAARRHGLPFAILRVVADPAH
                     RSLPNAALVAMRPDGEVDVGGVFGALSRDPWQVTMLARLAVDARRAFAALARARAMLG
                     ADFASRDLEEARAGRREETPARQSHSAVLAASIRNYAHDMA"
     gene            984..1484
                     /locus_tag="VKU03_06300"
     CDS             984..1484
                     /locus_tag="VKU03_06300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70911.1"
                     /translation="MLFRSLTLNRSSWKGALMGAALIASPAFAQTGPFADLQGAWAGD
                     GTISTNDGRSERIRCRAKYYVSPSGKNLDQQLRCASPSYRFDVNSGLVREPDGAIAGR
                     WTETTRNASGTVRASENGDAIVAQITGPSFTADMTLVTRGDGQQVQIVPNGSDIRSVS
                     VDLHRE"
     gene            complement(1668..6224)
                     /locus_tag="VKU03_06305"
     CDS             complement(1668..6224)
                     /locus_tag="VKU03_06305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ70912.1"
                     /translation="MNSQRGSHPADPTREFLAGGGEMGALMRGFDWSRSPLGPPEFWS
                     RGLKTAVRIMLTSRQPIWIGWGDELTYLYNDPYKSIIGGKHPWALGRPTAEVWREIWD
                     DIGPMLATAMKGDEGTYVEEQLLIMERSGYPEETYYTFSYSPIPDDDGAPGGIICANT
                     DDTRRVIGERQLTLLRELAAATADARTVERACQRSAEALATDPKDLPFAMIYLAEPDG
                     RTLSLAGSCGIAPNPVSAPQSAPLGVSAKWPFAAAIQDHQPVVVSDLAAHFGTEFPKG
                     AWDESPSQAALIPIPARGETARAGVLVAGLNPFRLFDDDYRRFLGLTAGEIGASVANA
                     QAYEEERRRADALAELDKAKTLFFSNVSHEFRTPLALMLGPLEEALAKPADRLSTENR
                     HLIEIAHRNGVRLLKLVNTLLDFSRIEAGRVQASYEPVDLAAFTADLASNFRSAVERA
                     GLRLVIDCPPLPGPVHVDRDMWEKIVLNLVSNAFKFTFEGEIAVEARPAADGASAEIT
                     VRDTGTGIPAAEIPHLFERFRRVEGARGRSFEGSGIGLALVQELVRLHGGTIHVDSEL
                     DRGSRFTVSLPFGVAHLPADRIGAARTQGTTRIRAESYVDEALGWMSESAGPAGDGPL
                     SPVSEDLGAIAPLPGATGQLVLLADDNADMRHYVQRLLTSAGFRVEAAPDGEAALASA
                     RRVKPDLVISDVMMPRLDGFGLLAALRNDPELREIPVLLLSARAGEEAKVEGLESGAD
                     DYLTKPFSAREMLARVETNLRLARLREESARALRDEALILEKLNETGAAIAAEIDLER
                     AVQVVTDAATALSGAAFGAFFYNVVDANGEALLLYALSGAPREAFASFPNPRNTQIFG
                     PTFRGEGVTRSPDITKDRRFGKNPPYYGMPKGHLPVRSYLAAPVFSHSGEVLGGLFFG
                     HPEAGRFDERAERLVVGIAIQAGIAIDKGRLYRSAQQEIEQRKRTEAALRESEESLER
                     KVAERTAELQAANDRLRAEAAERARAEDALRQAQKMESIGQLTGGVAHDFNNLLTVIS
                     GNLETIRRHLANPTHDRATLERAADNAMRGAQRAASLTQRLLAFSRQQPLEPKALNVN
                     RLVNGMSDLLRRTLGEQVAIETVLSGGLWRVHADPNQLEVAILNLAVNARDAMPEGGK
                     LTIETANAYLDENYAAAQAEVAPGQYVVVAITDNGAGMSREVIARAFEPFFTTKGIGQ
                     GTGLGLSQVYGFVKQSGGHVKIYSEVGHGTTVKIYLPRHHGADEAPDLSPSAAKAPRA
                     AQAETILVVEDDEDVRSYTTTLLRELGYGVVEAQDGEAALRALEASLEVALLFTDVGL
                     PGRLNGRQLAEEARRRRPGLKILFTTGYARNAIVHDGRLDPGVQLIAKPFTYAALAAK
                     LRDVLDARSPSGRILLVEDEALLQLLAADLLEELGFKVDTASTATEALNKLRLIPGGV
                     DAAIVDIGLPDRKGDALIAELRALFPSLPVVIASGQDEGAVREQVKSEADVSVLPKPY
                     TKDQIEAALREIGVTAKRNDAAGG"
     gene            6387..6683
                     /locus_tag="VKU03_06310"
     CDS             6387..6683
                     /locus_tag="VKU03_06310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70913.1"
                     /translation="MSLNSASRLQVMRMPNDPRYWFAAKRYGWGWGLPIAWQGWLVLA
                     AFIGLVVLGAFLFPPRKFLPEYVVYVVVLCALLIGVCWAKGEPPHWRWGDKEKP"
     gene            complement(6738..6813)
                     /locus_tag="VKU03_06315"
     tRNA            complement(6738..6813)
                     /locus_tag="VKU03_06315"
                     /product="tRNA-Phe"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6778..6780),aa:Phe,seq:gaa)
     gene            complement(6883..7725)
                     /locus_tag="VKU03_06320"
     CDS             complement(6883..7725)
                     /locus_tag="VKU03_06320"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ70914.1"
                     /translation="MIERSVDCLGPHGFHRVVYSQWDGPAGAPTLLCMHGLTRNGKDF
                     DVVAQALSSRYRVICPDAPGRGRSAHLAAPEDYSYPLYVADLATLIARLDVESLDWLG
                     TSMGGLIGMMIAAQPGSPIRRLAINDIGGFIPKAALERIGSYVGLDPSFPSLEAMEQA
                     VRQNYATFGPLTDAEMRRLTEISTKRRPDGSYGFAYDPRIAIAFKAAPFQDVDLWRVW
                     DAITCPVLLLRGETSDVLPRAVAEEMTRRGPKAKLVEFAGVGHAPALFSEEQIAPIRE
                     FLFG"
     gene            complement(7839..8543)
                     /locus_tag="VKU03_06325"
     CDS             complement(7839..8543)
                     /locus_tag="VKU03_06325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70915.1"
                     /translation="MKKRTIRIAFSAIVLAAAFGLAGTNAANAESVMKICGDQWKQAK
                     ASGTTNGMTWKDFLAKCREEQKGAAAPAAAPAPAASSTAPAATTAAAAGKTAKECNAE
                     YEANKGAIKAAGQKKKDFIAACRAGTETTPPAAAAPAPAPAAAPAPAATAPKPKPMAA
                     PKPMAAPTGAGEFQTEAEAKAKCPTDTVVWVNTKTGIYHFAGTRSYGKTKQGAYMCEA
                     DAKAAGRRASEKEKHP"
     gene            complement(8620..9270)
                     /locus_tag="VKU03_06330"
     CDS             complement(8620..9270)
                     /locus_tag="VKU03_06330"
                     /EC_number="1.14.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF025024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008198 - ferrous iron binding [Evidence
                     IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HLJ70916.1"
                     /translation="MEFCDFDLLERTPLQRDPFDYIVVPEFIRAERFAEIVADYPDVP
                     GPGSHPPAELEIRGQFAKLMDELRGAEFQAAIERKFDVDLTGRPTMYTVRGYVREKDG
                     AIHTDSATKIITVLLYMNERWQEDAGRLRLLRGPDNLDDYVAEVPPYGGTLLVFKRSD
                     NSWHGHKPFSGPRRAIQLNWVTSQDVVDSEQRRHQVSTRFKKFKQFFTHGRAAAGH"
     gene            complement(9426..10775)
                     /gene="accC"
                     /locus_tag="VKU03_06335"
     CDS             complement(9426..10775)
                     /gene="accC"
                     /locus_tag="VKU03_06335"
                     /EC_number="6.3.4.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_function: GO:0004075 - biotin carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase biotin carboxylase
                     subunit"
                     /protein_id="HLJ70917.1"
                     /translation="MFDKILIANRGEIALRILRAAKELGVATVAVHSTADAGAMHVKL
                     ADESVCIGPPPAKDSYLNIPSLLAACEITGADAVHPGYGFLSENARFADILAEHNIAF
                     IGPKAEHIRLMGDKIEAKRTAARLGIPCVPGSPGAVDDIAEAERIAAGIGYPVLVKAA
                     AGGGGRGMKVARSAAGLADAILTAKAEAKAAFGDDAVYLEKYLEKPRHIEIQVLGDGR
                     GRAIHLGERDCSLQRRHQKIWEEGPSPALNADQREEIGAVVAKAMADLGYVGVGTVEF
                     LYEDGRFYFIEMNTRIQVEHPVTEMITGIDLVNEQIKIAAGSPLTIAQKDVRLVGAAI
                     ECRVNAEHARSFTPSPGRISYYHPPGGLGVRVDSAVYQGYVIPPFYDSLVGKLVVHGR
                     TRNEALLRLRRALDEFIIDGVETTLPLFRALVRNADIQNGQYDIHWLENFLASEPVT"
BASE COUNT         1734 a         3470 c         3714 g         1912 t
ORIGIN      
        1 tcacaggaaa tcgaagccgc ggtcgcggcc ggggcgcgcc ggcttttgag ctttggcgtc
       61 gccggcgcgc tgatcccgca attgcagccg ggcgacctca tcctcgccca tggcgtgcgc
      121 gacgggcggc gcagcctgac ttgcgataag gactggcgca gcgccatcgg gaggcggctc
      181 caacggccgg gttcgccgcg ggccgcggcg tcggcggctt cggaggccgc cttgaaatcc
      241 gccctgttcc gtttcggtcg ccaaagcggc tggcgtccgg tcctcgacgc cgccgagcgc
      301 tatccgatcg ccgaaatcgc cggcgctgac gcccccgtcg cggatgaaat cggcaagagc
      361 gcgcttttcg ccgcgaccgg cgccgtggcg gtcgacatgg aatcggtgct ggtcgcgcgc
      421 gcggcgcgcc gccatggctt gcccttcgcc attttgcgcg tcgtcgccga tccggcgcat
      481 cgctccttgc ccaacgcggc tctggtcgcg atgcgccccg acggggaggt cgatgtcggc
      541 ggcgtcttcg gcgcattgag ccgcgacccc tggcaggtca cgatgctggc gcgcctggcg
      601 gtcgacgccc gccgcgcttt cgccgcgctc gcccgcgccc gcgccatgct gggagcggat
      661 tttgccagtc gcgatctcga agaggcccgc gccggccggc gcgaggagac gcccgctcgc
      721 cagtcgcaca gcgccgtcct ggccgcgtcg atccgcaatt atgcgcacga tatggcgtag
      781 cgcggcggaa atttgtcgaa gcagacctaa tgtcgatcct ccgcatcgga tgacgcaagg
      841 cgcgttccgc cgataaggga acgccgccga gcgagcggcg ttgagtgcgg gaagcgcgcc
      901 ggagcgacgc ccgcaacatt gttcgttttg cgagccgccg cagtgcgcga gaagtcgcgt
      961 gtctcaatgg aggttcaggc attatgctgt ttcgctcctt gacgttgaac cgctcatcgt
     1021 ggaaaggcgc gctgatgggc gcggcgctga tcgcatcgcc cgcttttgcg cagacaggac
     1081 cctttgccga tcttcaaggc gcttgggccg gcgacggcac gatttcgacg aatgacggac
     1141 gcagcgagcg catccgctgc cgcgccaaat attacgtttc gccgagcggg aagaatcttg
     1201 accagcagtt gcgctgcgcc agcccgagct accgcttcga tgtgaatagc ggccttgtcc
     1261 gcgaaccaga cggggcaatc gcgggccggt ggaccgagac gacgcggaac gccagcggca
     1321 ctgtcagggc gagcgagaat ggcgacgcca tcgtggcgca aatcaccggg ccgagtttca
     1381 ccgccgacat gactctcgtc acgcgcggcg acggccaaca ggtgcagatc gtcccgaacg
     1441 gctccgacat ccgatcggtg tccgtcgatt tgcatcgcga gtgatcggct cggcagtatc
     1501 ggcggatcag actggcgctc ggcgcgctgc gacatctcgc tcgatccgca gaatgggata
     1561 ggccgctggc ggacgatcga tctcacgtgc cttgtcgagc ggcgtctcgg cgtcaggcga
     1621 agcgctacgc gtgatgaggg caagggttcc cctgctacgc tcggcgctca gccgcccgcc
     1681 gcgtcgttgc gtttggcggt tacgccgatc tcgcgcaggg cggcctcgat ctggtctttc
     1741 gtataaggct tcggcagaac cgagacgtcg gcttcggatt tcacctgctc gcgcaccgcg
     1801 ccctcatcct ggccgctcgc gataacaacg ggcagggagg ggaagagagc gcggagctcc
     1861 gcgatcagcg cgtcgccttt gcggtcgggc aggccgatat cgacgatcgc ggcgtcgacg
     1921 ccgcccggaa tcaggcgcag cttgttcaga gcctcggtcg cggtgctggc ggtgtcgacc
     1981 ttgaagccga gctcctccag gagatcggcg gcgaggagct gaagcagcgc ctcgtcctcc
     2041 acgagcagga tgcggccgga cggcgagcgc gcatcgagca cgtcgcggag cttggccgcc
     2101 agggcggcgt aggtgaaggg cttggcgatg agctgcacgc cgggatcgag gcggccgtca
     2161 tgaacgatgg cgttgcgcgc gtatcccgtg gtgaacagga ttttgagccc gggcctgcgc
     2221 cggcgcgcct cctcggccaa ctggcgcccg ttcaatcgtc cgggcagacc gacatcggtg
     2281 aacaggagcg cgacttccag gcttgcctcc aacgcccgca acgcggcctc gccgtcctgc
     2341 gcctcgacga cgccgtagcc cagctccctg agcagcgtcg tcgtatagga gcgtacgtcc
     2401 tcgtcgtcct cgacgacgag aatcgtctct gcctgcgccg cgcgcggcgc cttcgcggcc
     2461 gaaggcgaaa ggtccggcgc ctcgtccgcc ccgtgatggc gcggcaggta gattttcacc
     2521 gtcgtgccgt ggccgacctc gctgtagatt ttgacgtggc cgccggattg tttgacgaag
     2581 ccgtagacct gcgacaggcc gagacccgtg ccctggccga tgcccttggt ggtgaagaac
     2641 ggctcgaagg cgcgcgcgat gacctcgcgg ctcatccccg cgccgttatc ggtgatcgcc
     2701 acgacgacat attggcccgg cgcgacctcg gcttgcgccg ccgcgtaatt ctcgtcgaga
     2761 taggcgttgg ccgtttcgat ggtcagcttg ccgccttccg gcatggcgtc gcgcgcattg
     2821 accgccagat tgaggatcgc cacttcgagc tggttgggat cggcgtggac ccgccatagg
     2881 cctcccgaca gcaccgtctc gatcgcgacc tgttcgccga gcgtgcggcg caagaggtcc
     2941 gacatgccgt tgaccagcct gttgacattg agcgccttgg gctcgagcgg ctgctggcgc
     3001 gagaaggcga gcagccgctg ggtgagggag gcggcgcgct gcgccccgcg catcgcgttg
     3061 tcggccgcgc gctccaacgt cgcccgatca tgcgtcgggt tggcgagatg gcggcggatc
     3121 gtctcgagat tgccgctgat cacggtgagc agattgttga aatcatgcgc cacgccgccg
     3181 gtgagctgac cgatgctctc cattttctgg gcttgccgca gcgcgtcctc ggctctcgcc
     3241 cgctcggccg cctcggcgcg caggcgatcg ttcgcggcct gcaattcagc ggtgcgctcc
     3301 gcgactttcc gctccagcga ttcttcgctt tcgcgcaagg ccgcttcggt tcgcttgcgc
     3361 tgctcgatct cttgctgggc cgagcgatag agccgcccct tgtcgatggc gatgccggct
     3421 tggatggcga tgccgacgac cagccgctcc gcccgctcgt cgaaacggcc ggcctcggga
     3481 tgaccgaaaa acaggccgcc gagaacctcg cccgaatgcg agaaaaccgg cgccgccaga
     3541 tagcttcgca ccgggaggtg ccctttaggc atgccgtaat agggaggatt cttgccgaag
     3601 cgccggtcct tggtgatgtc gggcgagcgc gtgacgcctt cgccgcggaa cgtcggtccg
     3661 aagatttgcg tgttacgcgg attgggaaag ctggcgaagg cttcgcgcgg cgcgccggac
     3721 agcgcgtaga gcagcaaggc ttcgccgttc gcgtcgacga cattgtagaa gaacgcgccg
     3781 aaagcggcgc cggaaagggc ggtcgcggcg tcggtgacga cctgcacggc gcgctccaag
     3841 tcgatctcgg ccgcaatcgc ggcgccggtc tcgttaagtt tttcaaggat caacgcctcg
     3901 tcgcgtagcg cccgagcgct ttcttcccgc agacgggcca gacggagatt cgtctcgacg
     3961 cgcgcgagca tttcgcgggc agagaacggc ttggtcaaat agtcgtcggc gccggactca
     4021 aggccctcca ccttcgcttc ctcgccggcg cgcgcggaaa gcagcaggac gggaatttcg
     4081 cgcaattcgg gatcattgcg caacgccgcg aggagaccga atccgtcgag cctcggcatc
     4141 atcacgtcgg aaatcacgag atcgggcttg acgcggcggg cggaggcgag cgcggcttcg
     4201 ccatccggcg ccgcctcgac gcgaaagccg gccgaggtca ggaggcgctg gacgtaatgg
     4261 cgcatgtcgg cgttgtcgtc cgccagcagg acgagctggc cggtcgcgcc gggcagcgga
     4321 gcgatggcgc cgaggtcttc tgaaaccgga gagagggggc cgtcgccggc cggaccggcg
     4381 ctttcgctca tccatcccaa ggcttcgtcg acatagctct cggccctgat ccgggtcgta
     4441 ccttgcgttc gcgcggcgcc gatccgatcg gccggcaaat gcgcgacgcc gaagggcagg
     4501 gagaccgtga agcggctgcc gcgatcgagt tcgctgtcga cgtggatcgt cccgccatgc
     4561 agccgcacca gttcctggac gagggcgagg ccgatgccgc tgccttcgaa ggaccggccg
     4621 cgcgcccctt cgacccggcg gaagcgctcg aagaggtgcg ggatttctgc ggccggaatg
     4681 ccggttccgg tgtcgcgcac cgtgatttcg gcggaggcgc cgtccgccgc cggcctcgcc
     4741 tcgaccgcaa tctcgccctc gaaggtgaat ttgaacgcgt tcgaaacgag gttgagaacg
     4801 atcttctccc acatgtcgcg atcgacatgg accgggccgg gcagcggcgg gcagtcgatg
     4861 accagacgaa ggccggcccg ctcgaccgcc gagcggaaat tggaggcgag atcggcggtg
     4921 aaggcggcca aatccaccgg ctcgtagctc gcctgcacgc ggcccgcctc gatgcgggaa
     4981 aaatccagaa gcgtgttgac gagcttcagc agccggacgc cgttgcgatg ggcgatctcg
     5041 atgagatggc gattttccgt ggagaggcga tcggctggct tagcgagcgc ctcctccagc
     5101 ggtccaagca tcaacgccag cggggtgcga aattcgtgac tgacattgga gaagaacagc
     5161 gtcttggcct tgtccagctc ggcgagcgcg tcggcgcgcc ggcgctcctc ctcataggcc
     5221 tgcgcgttgg cgacgctcgc gccgatctcg ccggcggtca ggcccagaaa ccggcggtag
     5281 tcgtcgtcga acaggcggaa cgggttgagg ccggcgacga ggacgccggc gcgcgcggtt
     5341 tcgcctcttg ccggaatggg gatgagagcg gcttgcgacg ggctctcgtc ccaggcgccc
     5401 ttcggaaact ccgtcccgaa atgggcggcg agatcgctga cgacgaccgg ctggtgatct
     5461 tgtatggccg cggcgaatgg ccatttcgcc gacacgccga gaggggcgga ttgcggcgcc
     5521 gagaccggat tcggcgcgat gccgcatgag ccggcgagcg acagcgtgcg gccgtccggc
     5581 tcagcgagat agatcatcgc gaagggcaag tccttcggat cggtggcgag cgcctcagcg
     5641 ctgcgctggc aggcccgctc gaccgtccgc gcatcggcgg tcgcggcggc caattcgcgc
     5701 agcagcgtca actgccgctc gccgatgacc cggcgcgtgt cgtcagtatt ggcgcaaatg
     5761 atcccgcccg gcgcgccgtc atcgtccggg atcgggctat aggagaacgt atagtaggtc
     5821 tcttccggat agccgctgcg ctccatgatc aggagctgct cttcgacgta agtgccctcg
     5881 tcgcccttca tcgcggtcgc cagcatcggg ccgatatcgt cccagatttc ccgccaaacc
     5941 tccgctgtcg ggcggccgag cgcccaagga tgcttgccgc cgatgatcga cttgtaggga
     6001 tcgttgtaga gataggtcag ctcgtcgccc cagccgatcc aaatcggctg gcgcgaggtg
     6061 agcatgatcc gcaccgcggt tttcaggccg cgcgaccaga attcgggcgg cccgagcggc
     6121 gaacggctcc aatcgaagcc gcgcatcagc gcgcccatct cgccgccgcc ggcgaggaat
     6181 tcccgcgtcg gatcggccgg atgcgaacct ctctgagaat tcattccaac gcccaacgcg
     6241 aggccgaaga gaaaagtcta gccacggctc gggaaaattt gaagacgcgc cggacgcgcg
     6301 ccaggccggc gcggcgctct ctccgacggg ttaagcgccg gccggacggt cgcgtcgcga
     6361 ttcaagccgc gtgaccgctc gcctcaatga gcctaaattc ggcttcgcga ttgcaggtca
     6421 tgcgcatgcc gaacgaccct cgatattggt ttgccgccaa acgctatgga tggggatggg
     6481 gtctgccgat cgcgtggcaa ggctggctgg ttttggccgc cttcattggt ctcgttgtcc
     6541 tcggcgcgtt tctgtttccg ccgagaaaat ttcttcccga atatgtcgtc tatgtcgtcg
     6601 tcctttgcgc cctgctcatc ggcgtgtgct gggcgaaagg cgagccgccg cattggcgat
     6661 ggggagataa agaaaaaccc taaaacctat tcgcgggctc gttctcaaaa aagctcgcga
     6721 aacgggagac ggggtcatgg tgcccagggg cggaatcgaa ccaccgacac tgcgattttc
     6781 agtcgcatgc tctaccaact gagctacctg ggcgtcgccg gcgcggcggg ccgcggcggc
     6841 gggcggttta taggtaggcg cggcgggctt gtccagcgcc cttcagccga agagaaactc
     6901 gcggatcggc gcgatctgct cctcggaaaa aagcgccggc gcgtggccga ccccggcaaa
     6961 ttcgacgagc ttcgccttgg ggccgcgccg ggtcatctct tcggcgaccg cgcgcggcag
     7021 cacgtccgag gtttcgccgc gcagcagcag gaccggacat gtgatcgcgt cccatacccg
     7081 ccataaatcg acgtcctgga agggcgccgc cttgaaggcg atcgcgattc tcggatcata
     7141 ggcgaagcca taggagccgt cgggccgccg cttcgtgctg atttcggtga ggcggcgcat
     7201 ctcagcgtcg gtgagcggcc cgaatgtcgc ataattctgc cgcaccgcct gctccatcgc
     7261 ttcgagcgag gggaagctcg ggtccagtcc gacataagag ccgatgcgct ccaaggcggc
     7321 tttggggatg aatccgccga tgtcgttgat cgccagacgg cggatcggcg agcccggctg
     7381 ggcggcgatc atcatgccga tcagcccgcc catcgacgtg ccgagccaat cgagggattc
     7441 gacgtcgagc cgggcgatca gcgtcgccag gtcggcgaca tagaggggat aggaataatc
     7501 ttccggcgcg gccaaatggg cgctgcgtcc tcgcccgggg gcatccggac aaatcacgcg
     7561 ataacgcgac gagagcgctt gcgcgacgac atcgaaatcc tttccgttcc gcgtcaggcc
     7621 gtgcatgcag agcagagtcg gcgcgccggc cggcccatcc cattggctat agacgacacg
     7681 gtgaaatccg tgcggtccga ggcagtcgac gcttcgttcg atcatgtcta tcgcttcgat
     7741 gaagccggag aggacatcgg gcgcttcagc ctagtccgcg atgcgccgcg cccaatcagg
     7801 cgcggcgcga tgatgagcgc cgatgcgctg cgctggcctt atggatgctt ttctttttcc
     7861 gacgcgcggc ggccggcggc cttggcgtcg gcttcgcaca tataggcgcc ttgcttggtc
     7921 ttgccgtagc tgcgcgtgcc ggcgaaatgg taaatgccgg tcttggtgtt gacccagacg
     7981 accgtatcgg tcgggcattt ggccttggct tcggcttcgg tctggaattc gccggcgccg
     8041 gtgggcgcgg ccatcggctt gggcgcggcc atcggctttg gcttcggcgc ggtggcggcc
     8101 ggggccggcg ccgctgcggg agcaggcgcg ggcgctgctg cggcgggcgg cgtggtttcc
     8161 gttcccgccc ggcaagcggc gatgaaatcc ttcttcttct ggccggcggc tttgatcgcg
     8221 cccttattcg cctcgtattc ggcgttgcat tccttggcgg tcttgccggc cgcggcggcg
     8281 gtcgtcgcgg ccggcgctgt ggaggacgcg gccggggcag gggcggcggc cggcgccgcg
     8341 gcgcctttct gctcctcgcg gcacttggcg aggaaatcct tccaggtcat gccgttggtc
     8401 gttccgctgg ccttggcctg cttccattga tcgccgcaga ttttcatcac gctttcggcg
     8461 ttggccgcat tggtcccggc gaggccgaaa gccgccgcca ggacgatcgc cgaaaaggcg
     8521 atcctgatcg tacgtttttt caaggcatgc tccctagttc gacccgcccg tcattggaga
     8581 ggccaagcgg ctcagtctgg ccttgggtcg catcaagcgt caatggcccg cggcggcgcg
     8641 tccgtgggta aagaattgct tgaatttctt gaaccgggtc gagacctgat ggcgacgctg
     8701 ctcggaatcg acgacgtcct ggctcgtcac ccaattgagc tggatcgcgc ggcgcgggcc
     8761 ggaaaagggc ttgtggccgt gccaggaatt gtccgagcgt ttgaagacca gcaaagtgcc
     8821 gccatagggc ggcacttcgg cgacatagtc gtcgagattg tcggggccgc gcaacaggcg
     8881 caggcgtccg gcgtcctcct gccagcgctc gttcatatag agaagaacag tgatgatctt
     8941 tgtggcggaa tcggtatgga tcgcgccgtc cttttcgcga acatagccgc gcacggtgta
     9001 cattgtcggc cggccggtca gatcgacgtc gaatttgcgc tcgatcgcgg cttggaattc
     9061 cgcgccgcgc aattcgtcca tcagcttggc gaactggccg cgaatttcaa gctcggccgg
     9121 cgggtgcgag ccggggcccg gtacgtcggg ataatcggcg acgatctcgg cgaagcgttc
     9181 ggcgcgaatg aactccggaa ccacgatata gtcgaaaggg tcgcgctgca gcggcgtgcg
     9241 ctccagcaaa tcgaaatcgc aaaattccat cgtcggctcc acggagcggc cgccgatcac
     9301 ggcgccgcgt cggcggcgcc gcttatatca agcgcgccgc ggcgcgccaa atgcgcgcta
     9361 gaatcgttct cattttcatt ttttcgcggc agagcgggcg tcgagcccaa gcttctgcaa
     9421 gagcctcacg tcacgggctc ggatgcgaga aaattttcga gccagtgaat gtcgtattgg
     9481 ccgttttgga tatcggcgtt acgcaccaaa gcgcggaaca acggcagggt cgtctcgacg
     9541 ccgtcgatga tgaattcgtc gagcgcgcgg cgcagccgca gcaaggcctc gttgcgcgtc
     9601 cggccatgga ccaccagctt gccgaccagc gaatcgtaga agggcgggat cacatagcct
     9661 tgatagaccg ccgaatcgac gcgcacgcca aggccgcccg gcgggtggta ataggagata
     9721 cggcccggcg acggcgtgaa gctgcgcgca tgctcggcgt tgacgcggca ttcgatcgcc
     9781 gcgccgacga gacgtacgtc cttctgcgcg atcgtcagcg gcgaccccgc ggcgatcttg
     9841 atttgctcgt tgacgagatc gatgccggtg atcatctcgg tcaccgggtg ctcgacctgg
     9901 atgcgcgtgt tcatctcgat gaaatagaac cgcccgtcct cgtaaaggaa ttcgaccgtg
     9961 ccgacgccga catagccgag atcggccatg gctttggcga cgaccgcgcc gatctcctcg
    10021 cgctgatcgg cgttgagcgc cggcgagggg ccttcctccc agattttttg gtggcggcgc
    10081 tgcagcgagc aatcgcgctc gccgagatgg atggcccggc cgcgcccgtc gcccaaaacc
    10141 tggatttcga tatggcgcgg cttttcgaga tacttttcga gatagacggc gtcgtcgccg
    10201 aaagcggcct tggcttcggc cttggcggtg aggatcgcgt cggcgaggcc ggcggcgctg
    10261 cgcgcgactt tcatgccccg cccgccgcct ccggccgccg ccttgaccag gaccggatag
    10321 ccgatgccgg cggcgatccg ctcggcctcg gcgatgtcgt cgacggcgcc tggcgatccc
    10381 ggcacgcaag gaatgccgag ccgcgcggcg gtgcgcttgg cttcgatctt gtcgcccatc
    10441 agccggatat gctcggcctt cggcccgata aaggcgatgt tgtgctcggc gaggatgtcg
    10501 gcgaagcgcg cattttcgga gaggaagccg tagccgggat ggacggcgtc ggcgccggtg
    10561 atctcgcagg cggcgagcag cgaggggatg ttgagatagc tgtccttcgc cggcggcggg
    10621 ccgatgcaga cgctctcgtc ggcgagcttg acgtgcatgg cgccggcatc ggcggtcgaa
    10681 tgcaccgcga cggtggcgac gccaagctcc ttggcggcgc gcagaatgcg cagggcgatc
    10741 tcgccgcgat tggcgatgag gatcttatcg aacatgccgg gagcctttgt ttggcgccat
    10801 ggcgctcgac gcccgcccga tcactcgatg
//
LOCUS       DATGJW010000204        12727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_141635, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000204 DATGJW010000000
VERSION     DATGJW010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12727
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141635"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(219..1790)
                     /locus_tag="VKU03_06340"
     CDS             complement(219..1790)
                     /locus_tag="VKU03_06340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter"
                     /protein_id="HLJ70918.1"
                     /translation="MTGGLNWTAAIVFVAFFALVTVIGFLASRWKAGDLNHLHEWGLG
                     GRRFGAWITWFLLGGDLYTAYTVIAIPSLVYAAGAFGFFAVPYTIVIYPFLYLTLPRL
                     WAVSHKRGYLTAGDFVAGRYGSKGLEFAVALTGILATMPYIALQLVGMERVIQALGFP
                     HEGPFAHVPLTLAFIILALYTYKSGLRAPAMIAFVKDAMIYIFVIAVVIAVPIKLGGF
                     GPIFDAAGKAYAAKVAANPKAAAGLLLAPQQIGPYITLALGSAMALFMYPHALTGTLA
                     ASSGEAIRRNTMTLPAYSVLLGLIALMGMMAHAAGITVKNPQDAVPQLLLKMFPDWFA
                     GFCFAAIAIGALVPAAVMSIGAANTFTRNIWKPFVHPEMTPGEESFLAKLVSLIVKIG
                     ALAVILFIPTQFAVDLQLLGGVWMVQIFPAIIFGLFTRWYGGPALLIGWAVGMIVGTA
                     LSWGPTAWVAVHPLAWDIPLVGKLDLGLGFAAYNGLTAVIVNMVVATVLSALWRTRGA
                     DETEAADYDDARVAA"
     gene            complement(1787..1963)
                     /locus_tag="VKU03_06345"
     CDS             complement(1787..1963)
                     /locus_tag="VKU03_06345"
                     /inference="COORDINATES: protein motif:HMM:NF023183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HLJ70919.1"
                     /translation="MKYILLFAPCVVALVAPLYNSVDPALAGIPFFYWFQLLLILVSA
                     FCIYLADRIAKARP"
     gene            complement(2072..2521)
                     /gene="aroQ"
                     /locus_tag="VKU03_06350"
     CDS             complement(2072..2521)
                     /gene="aroQ"
                     /locus_tag="VKU03_06350"
                     /EC_number="4.2.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003855 - 3-dehydroquinate dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II 3-dehydroquinate dehydratase"
                     /protein_id="HLJ70920.1"
                     /translation="MSRLVYVLNGPNLNLLGQRQPEIYGRESLADIEAACRAAAGALK
                     LDIKFCQSNREYELIDWIHEARGTAAGIVINPAAFTHTSVAILDALSAYDGPVVEVHL
                     SNIHKREAFRHHSFVSTRADGVIAGFGGQGYEFALRRIARLIDAKKA"
     gene            2708..3730
                     /locus_tag="VKU03_06355"
     CDS             2708..3730
                     /locus_tag="VKU03_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HLJ70921.1"
                     /translation="MAAAKTKLIIDVDTGIDDALALLHACASPEADILGVTTVSGNVD
                     LRRATRNTRAVLALAGRGDIPVWPGCARPILREAQDASYVHGGSGLGQAELPDPPPAR
                     DERHAVDQILALAKAHQGELVLVPTGPLTNIAAAVTREPALVKWVKRLVLMGGSFQGG
                     GNTTPAAEFNIWHDPEAARIVFNAFAQEDAAPLVAVGLDVTQKVLLLPEQIAALGRRI
                     AHTPRGPALARFFDDATRDYFDFIEKRLGQRFFVMHDPLAVAAALDPSLIETQRVTVD
                     IETTGALTSGMTVADWRGVWKRKPNVEVATGVRAEAAIAQFLERMERLAGKSLLPREA
                     GEGDRA"
     gene            complement(3767..4507)
                     /locus_tag="VKU03_06360"
     CDS             complement(3767..4507)
                     /locus_tag="VKU03_06360"
                     /inference="COORDINATES: protein motif:HMM:NF019031.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-lyxose/D-mannose family sugar isomerase"
                     /protein_id="HLJ70922.1"
                     /translation="MLRSFIDRRVEAMLEFCERCGFKLPPFALWSGQDFRAAPDAAKR
                     IAENGLGWNIVEFKRGAFARDGLSVFTLRMGDWRRLKSGAGRLYSEKALYAEDGQRTP
                     HHYHVVKTEDILNRGGARFVVELFKVDRAGRRLDERVRIMKDAAMIEVAAGGRVVLEP
                     GESLVLEPYVAHAFWAEGGAALAGEVSLANDDATDNYFLSAPEPPAPIEEDAPMRHPT
                     VRDYAALILPREAGEGDHAKHGGGGSGG"
     gene            complement(4524..5061)
                     /gene="ppa"
                     /locus_tag="VKU03_06365"
                     /pseudo
     CDS             complement(4524..5061)
                     /gene="ppa"
                     /locus_tag="VKU03_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009734779.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0004427 - inorganic diphosphate
                     phosphatase activity [Evidence IEA];
                     GO_process: GO:0006796 - phosphate-containing compound
                     metabolic process [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inorganic diphosphatase"
     gene            complement(5150..5656)
                     /locus_tag="VKU03_06370"
     CDS             complement(5150..5656)
                     /locus_tag="VKU03_06370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ70923.1"
                     /translation="MVETLVTIRTARPGDEAKIADVHDAAWRDAYRGIIPGRELERMI
                     ARRGPVWWRNAIARGSRLLVLDFNDRIAGYASYGRNRVPALAYGGEIFELYLAPEFQG
                     VGFGRRMFEAAQKDLADHGYRSFLIWALADNVRALGFYSRLGGRIVRRAPEKFGEETR
                     ERVAFGFD"
     gene            5834..6655
                     /locus_tag="VKU03_06375"
     CDS             5834..6655
                     /locus_tag="VKU03_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3108 domain-containing protein"
                     /protein_id="HLJ70924.1"
                     /translation="MDACSRAFSFRRAAFLMTAMLATLAGVAGARAETWRAVYNVSLI
                     GLPIGTADVNGQVSPTAYRIEANAKIVGLATMLSNAKGAATGAGAIAGGRVLPASFAT
                     TAANSKMTRTVRMALNEGVVTGVDISPPVEEKPGRVPLRQQDERGIVDPVGAAILPVA
                     ANQPLIGPAACNRSIPIFDGYTRFDLRLTYVGQRRVAVKGYSGPVVVCSARYVPIAGH
                     NPDRPAVKFMINNKQMEVWLAPIEPAHVLAPFRASVGTMIGTLVVEASEFRLDGK"
     gene            complement(6662..7114)
                     /locus_tag="VKU03_06380"
     CDS             complement(6662..7114)
                     /locus_tag="VKU03_06380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254548.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1178 family protein"
                     /protein_id="HLJ70925.1"
                     /translation="MIKYALVCEKDHSFESWFPDSDSFDRLLRRGLVACPECDSSRVA
                     KALMAPAVVGAKKAEAPAAAESQPVNVALVDERQRRLRELARELRQEIITKTDDVGRR
                     FPEEARAIHAGDAPPRSIRGQATLEEARALVEEGVGVLPVPQIPDELN"
     gene            complement(7118..7375)
                     /gene="grxC"
                     /locus_tag="VKU03_06385"
     CDS             complement(7118..7375)
                     /gene="grxC"
                     /locus_tag="VKU03_06385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin 3"
                     /protein_id="HLJ70926.1"
                     /translation="MPPITIYTTATCPYCLAAKALLRRKNAPFVEIGVDGDAKARAAM
                     AERAHGRRTVPQIFIGERHIGGCDDLHALDAAGELDPLLAG"
     gene            complement(7444..8241)
                     /locus_tag="VKU03_06390"
     CDS             complement(7444..8241)
                     /locus_tag="VKU03_06390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HLJ70927.1"
                     /translation="MLGLSFDAGPHAAPLWPRRALSAAAALGRAALDFVYPPTCLACR
                     AAVAESGALCPRCWREMRFIERPFCERLGTPFEQDLGPGLISPQAMADPPAYGRARAA
                     ARFEDGPVRRLVHRLKYSDRIELARPIGRWMARAGADILVEADILTPVPLHPLRLFAR
                     RFNQAALLAAAIGEQAGKPCDLTLLARVKATKAQVGLSRAQRAENVQGAFRVLSGAEA
                     RVKDKRIVLVDDVLTSSATANAATRALLRAGAASVDILVFARVVTGA"
     gene            8304..9191
                     /locus_tag="VKU03_06395"
     CDS             8304..9191
                     /locus_tag="VKU03_06395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973656.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLJ70928.1"
                     /translation="MAAPEIFDASLARARLERAARRGAADADFLLRRAAADFAERLAL
                     VKRRFAVAVDIATPGPHGAEALIRDPKIGFAARAAPSAPGPARRAAPSFVGDVERLAL
                     APRSCDLVSSLLALQGVNDLPGTLIQIRQALRPDGLLLACLLGGDTLTELRQSLTAGE
                     AEILAGAAPRVAPFADVRAMGALLQRAGFALPVVDLERVVVRYPDMIALMRDLRAMGA
                     TNVLIARSRRFLRRDVLARAGQIYAERFADPDGRLRATFDLIWLSGWAPHESQQQPLK
                     PGSATSRLEDAVKAARKGS"
     gene            9397..10236
                     /gene="cobS"
                     /locus_tag="VKU03_06400"
     CDS             9397..10236
                     /gene="cobS"
                     /locus_tag="VKU03_06400"
                     /EC_number="2.7.8.26"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903075.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008818 - cobalamin 5'-phosphate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylcobinamide-GDP ribazoletransferase"
                     /protein_id="HLJ70929.1"
                     /translation="MLQHRSSHSSGSLAPLFKNFAARTLEALRFYSRLPVPAFGFERA
                     PFAVPDLKRLAPYAPLAGAIIAACGAFVLWIAQALALPPLVAAALALSALIMAAGGLH
                     EDGLADVADGFGGGATRERKLDIMRDSRVGAYGAAALVLSLLLRAAALAALIGESAAI
                     GAAALLLAGALSRACSLAPLALLDPARPDGAGASVGRLGADAFGKSIAASLALAAILG
                     LGVLGLGRALLAFVLAWAAALAIVAIARRQIGGQTGDVGGAAQQLAEIACLIGLLIGA
                     GAA"
     gene            10246..10464
                     /locus_tag="VKU03_06405"
     CDS             10246..10464
                     /locus_tag="VKU03_06405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012452776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1289 domain-containing protein"
                     /protein_id="HLJ70930.1"
                     /translation="MPSPSTPCLKICVLDPLSGLCIGCGRTAEEIAAWPALSETERLA
                     VMAGLEARLRGARSRKARGGRVATRERG"
     gene            10464..11162
                     /locus_tag="VKU03_06410"
     CDS             10464..11162
                     /locus_tag="VKU03_06410"
                     /EC_number="3.4.23.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02281.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02281 family clan AA aspartic protease"
                     /protein_id="HLJ70931.1"
                     /translation="MRYLLPIGILVLAGLLLLAVPAETPLLGLDHKRFAFAALGAALF
                     TAFLSRIGSRRVALGDLARALGGALIWLVLIVGLTGVYAYRFEFGDIADRVIAELSPG
                     DVTIGKGGEVIVPRRLGGQFIIAAKVDNVPTSFLFDTGASTVVLRAEDARKMGVDVAN
                     LDYDVDVTTANGATRAAEAVLDRIAIGPIVVRNVHALIARPGALNENLLGANFLDRLQ
                     SYSVERGRLVLKGR"
     gene            complement(11203..11985)
                     /locus_tag="VKU03_06415"
     CDS             complement(11203..11985)
                     /locus_tag="VKU03_06415"
                     /EC_number="3.6.1.26"
                     /inference="COORDINATES: protein motif:HMM:NF003986.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol diphosphatase"
                     /protein_id="HLJ70932.1"
                     /translation="MPTLLLGWRGFCFALFAAFVVAASIVATRAGDRLALWRIVHDKC
                     VPDEKANGSPAPCAAVDLSAGEERGAAILKDLVGVAQFLAIPTRRLAGIESPELLDAD
                     APNYWRAAWAARGAMEKLLGRPLPREAVGMAINSSLARSQDQLHIHIDCVAPQTRAAL
                     DARLDELTAEWRPLSVLLARRRYVARRLDSFDLSDADPFRLLADGDPGAATHMALETL
                     AVIGARFKDQDGFVLLADHADPSAGDMAHGEDLLDHSCAIVR"
     gene            complement(11993..>12727)
                     /locus_tag="VKU03_06420"
     CDS             complement(11993..>12727)
                     /locus_tag="VKU03_06420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLJ70933.1"
                     /translation="RLLEKQGFKFRLGHKVTKIDKGGAGLKATIEPAEGGEAVTLETD
                     VALIAIGRRPYTDGLGLEAVGVKTERGQVAIDQNFRTNVEGIYAIGDVVRGPMLAHKG
                     EDEGVAVAEILAGQHGHVNYEVIPSVVYTSPEIASVGKTEEELKSAGIAYNIGKFPFS
                     ANGRARSMRKTDGFVKILADAATDRVLGAHIMGFGAGEMIHEAAVLMEFGGSAEDLAR
                     TCHAHPTLSEAVKEAALGVAKRTIHL"
BASE COUNT         2141 a         4270 c         4259 g         2057 t
ORIGIN      
        1 cccttcttct atgagcgcaa ccgcgtctgg cgccgggatg agctgtgaac ccttctcccc
       61 gtgcaggaga aggtggcgcg aagcgccgga tgaggggcgg cgcaggactc gaaaagtacg
      121 ggcagcgcga aaagcaaatc agcaaccctc ggcgcagccc cttatccggc cgtgcttcgc
      181 acggccacct tctccccgca aagcggggag aagggttact acgccgcgac gcgggcgtcg
      241 tcgtagtcgg cggcctcggt ttcgtccgcg ccgcgcgtgc gccacagcgc cgagagaacc
      301 gtcgccacga ccatgttcac gatcaccgcg gtgaggccgt tataggcggc gaagccgagg
      361 ccaagatcga gcttgccgac gagcggaatg tcccaagcga ggggatggac cgcgacccag
      421 gcggtcggcc cccacgacaa agcggtgccg acgatcatgc cgacggccca gccgatcagc
      481 agcgctggcc cgccgtacca gcgcgtgaac agaccgaaaa tgatcgccgg gaaaatctgc
      541 accatccaga cgccgccgag caattgcaga tcgaccgcga attgcgtcgg gatgaacagg
      601 atgacggcga gggcgccgat cttgacgatc agcgagacca gcttggcgag aaaggattct
      661 tcgcccggcg tcatctccgg atggacgaaa ggcttccaga tgttgcgcgt gaacgtattg
      721 gcggcgccga tcgacatgac cgcggccggc accagcgcgc cgatcgcgat cgcggcgaag
      781 cagaagccgg cgaaccagtc ggggaacatt ttcaggagca gttgcggcac cgcgtcttgc
      841 gggttcttca ccgtgattcc cgccgcatga gccatcatgc ccatcagcgc gatcaggccg
      901 agcagcaccg aataggccgg cagcgtcatc gtgttgcgcc ggatcgcctc gccgctcgat
      961 gcggcaagcg tgccggtcag cgcatgcggg tacatgaaca gcgccatggc cgagccgagg
     1021 gcgagcgtga tgtaagggcc gatctgctgc ggcgcgagca ggagccccgc ggcggccttc
     1081 ggattggccg cgaccttggc cgcgtaggcc ttgccggcgg cgtcgaaaat cgggccgaag
     1141 ccgccaagct tgatcggcac ggcgatcacc acggcgatga cgaaaatata gatcatcgcg
     1201 tctttgacga aagcgatcat cgccggcgcg cgcaagccgc tcttataggt gtagagcgcc
     1261 aggatgatga aggcgagcgt cagcgggaca tgcgcgaacg ggccctcatg aggaaagccg
     1321 agcgcctgga tgaccctttc catgccgacg agttgcagcg cgatgtaggg catcgtcgcg
     1381 agaatgccgg tgagcgcgac ggcgaattcg agtcccttgc tgccatagcg gccggcgacg
     1441 aaatcgccgg cggtgagata gccgcgcttg tgcgacaccg cccagaggcg cggcagggtc
     1501 aaatagagaa acggatagat gacgatcgta taagggacgg cgaagaagcc gaatgcgccg
     1561 gcggcataaa cgaggctcgg aatcgcgatc accgtatagg cggtgtaaag atcgccgccg
     1621 agcaggaacc aggtgatcca ggcgccgaaa cgccggccgc caaggcccca ttcgtgcaga
     1681 tggttgaggt cccccgcctt ccagcgcgag gcgagaaagc cgatgaccgt gaccaaagcg
     1741 aagaaggcga cgaagacgat cgccgcggtc cagttgaggc cgccggtcat ggccgcgcct
     1801 tggcgattcg gtcggcgaga tagatgcaga aggccgagac gaggatcagc aagagctgaa
     1861 accaatagaa gaaaggaatg ccggccagag cgggatcgac ggaattatag agcggcgcga
     1921 cgagggcgac gacgcagggc gcgaaaagca gaatatattt catcttcccc cccggcgggc
     1981 cggcacgtgc tgcggcgctt cgacggcggc cttctttcgc tcttagccaa ttatcccggc
     2041 aaggtcgaga gcggccgaag ggcttttacc ctcaagcttt cttcgcgtcg atcagccgcg
     2101 cgatccggcg cagcgcgaat tcgtagccct ggccgccgaa gccggcgatg acgccgtccg
     2161 ccctcgtcga gacgaaggaa tgatggcgga aggcttcgcg cttatggatg ttggaaagat
     2221 gcacctcgac gaccggcccg tcataggcgc tgagcgcgtc gagaatggcg acggaggtat
     2281 gggtgaaggc ggccggattg atgacgatcc cggcggcggt cccgcgcgcc tcgtgaatcc
     2341 agtcgatcag ctcgtattcg cgattgctct gacagaattt gatgtcgagc ttcagcgcgc
     2401 cggccgcggc gcggcaggcg gcttcgatgt cggcgaggct ctcgcggcca tagatttccg
     2461 gctggcgctg gccgagcagg ttcaggttcg gcccgttcag aacatagacg aggcggctca
     2521 tccggctcct ctctcgttcg gcgcggccgg cgcggtcagc cggcgatttc catgtgagcg
     2581 gagcctggat agaacaattc gccgcctgcg acaaatcgcc gaggcgacga tggggctcga
     2641 aacgcgacgg attttctgct tttgaaaggc ggcgcgcttt gcgccgtccc ttcgtagcga
     2701 ggatcccatg gccgcggcca agacgaaact gatcatcgac gtcgataccg gcatcgacga
     2761 cgctttggct ttgctgcacg cctgcgcctc gccggaggcc gacattctcg gcgtcacaac
     2821 ggtttccggc aatgtcgacc ttcgccgcgc gacgcgcaac acgcgcgccg tcctcgcctt
     2881 ggccgggcgc ggcgacattc cggtctggcc gggctgcgcc cggccgatcc tgcgcgaagc
     2941 gcaagacgcc agctacgtcc atggcggatc gggcctcggc caggcggagc tgcccgaccc
     3001 gccgccggcg cgcgacgagc ggcacgcggt cgaccagatc ctcgcgctcg ccaaggccca
     3061 tcaaggagag ctcgtcctgg ttccgaccgg gccgctcacc aacatcgccg ccgcggtgac
     3121 gcgggagccc gctctcgtca aatgggtcaa gcgcctcgtc ctgatgggcg gctcgttcca
     3181 gggcggcggc aacacgacgc cggccgccga attcaacatt tggcacgatc ccgaggcggc
     3241 gcgcatcgtc ttcaacgcct tcgcgcaaga ggacgccgcg ccgctggtcg cggtcggcct
     3301 cgacgtgacg caaaaagtac tgctgttgcc ggagcagatc gcggcgctcg gccgtcgcat
     3361 cgcccacacg ccgcgcggcc cggcgctggc gcgtttcttc gacgacgcca cgcgcgatta
     3421 tttcgacttc atcgaaaagc gcttggggca gcgctttttc gtgatgcatg atccgctcgc
     3481 ggtcgccgcg gcgctcgatc cgtcgctgat cgaaacgcag cgcgtaaccg tcgacatcga
     3541 gacgaccggc gcgctgacca gcggcatgac cgtcgccgat tggcgcggcg tctggaagcg
     3601 caagccgaac gtcgaggtgg cgaccggcgt cagggccgag gcggcgatcg cgcagttctt
     3661 ggagcggatg gagcggttgg cgggcaagtc gctcctcccc cgcgaagcgg gggaggggga
     3721 ccgcgcgtag cgcggtggag ggggcgtcgg cgtctgagga tttatctcag ccaccggagc
     3781 cccctccacc atgcttcgca tggtccccct cccccgcttc gcgggggagg ataagagccg
     3841 catagtctcg cacggtcgga tggcgcatgg gcgcgtcttc ttcgatcggc gccggcggct
     3901 cgggcgccga caggaaatag ttgtccgtcg cgtcgtcatt ggcgagcgag acttcgccgg
     3961 ccaaagccgc gccgccctcg gcccagaagg cgtgcgcgac gtaaggctcc aggaccaggc
     4021 tttcgcccgg ctcgaggacg acccgcccgc cggcggcgac ctcgatcatc gccgcatcct
     4081 tcatgatacg tacgcgctcg tccagcctgc gtccggcgcg gtccaccttg aacagctcga
     4141 ccacgaagcg cgcgccgccg cggttcaaaa tgtcctcggt cttgacgacg tgatagtgat
     4201 gcggcgtacg ttggccgtcc tcggcgtaca gggccttctc gctatacagc cgcccggcgc
     4261 cgctcttgag ccggcgccaa tcgcccatgc gcagcgtaaa gacgctgagg ccatcgcgcg
     4321 cgaaagcgcc gcgcttgaac tcgacgatgt tccagccaag cccgttctcg gcgattcgct
     4381 tcgccgcgtc cggcgcggcg cgaaaatcct gcccgctcca caacgcgaaa ggcggcagct
     4441 tgaagccgca gcgctcgcag aattcgagca tcgcctcgac gcggcgatcg atgaaggagc
     4501 gcagcatgcg cttgagctcc ggatcagtcc gcggctttcg ccttggcgcg ctcgatcgcc
     4561 gcggcgatca agcgatgcgc ctcgtcggcg ccgccagcga atgatcttga cccattttcc
     4621 aggctcgaga tccttatagt gggcgaagaa atgctcgatc tgcttcaagg tgatttcggg
     4681 cagatcgacg tagctcgaaa tgccggcata acgctgagtc agtttcgccg ccggcaccgc
     4741 gatgatcttt tcatcgccgc cggcttcgtc ctccatcaac agtacgccga cgaccttgca
     4801 gctcatgatc gcgccgggaa cgatcgccct cgtattggcg acgatgacgt cgcaaggatc
     4861 gccgtcttcc gagagagtat gaggaatgaa accgtaattt cccggatagc gcatcgacgt
     4921 atagagaaac cggtcgacga cgagcgcgcc gatcttcttg tccatctcgt atttgatcgg
     4981 ctcgccgccg atcggtactt cgatgaccac attgacctcg cgcggcgggt ccttgccgac
     5041 cggaatggct tcgagattca tcgcccctcc ccgcccttca tccgcgcgcg cgaccggcgc
     5101 cgccgaccgc gtcgctcgct ccggcgctgc gcaaccgaaa ttcagccaat cagtcgaaac
     5161 cgaaggcgac gcgctcgcgc gtctcctcgc cgaacttttc cggcgcgcgc cggacgatcc
     5221 ggccgccaag gcgcgaatag aagccgagcg cgcgcacatt gtcggcgagc gcccagatca
     5281 ggaacgaacg atagccgtga tcggcaaggt ccttctgcgc cgcttcgaac atgcgccggc
     5341 caaagccgac cccttgaaat tccggcgcga ggtagagttc gaaaatctcg ccgccatagg
     5401 cgagggccgg cacgcggttg cggccatagc tggcgtagcc ggcgatgcga tcattgaagt
     5461 cgagaaccaa aaggcgcgag ccgcgcgcaa tcgcattgcg ccaccagacc ggtccgcggc
     5521 gcgcaatcat tcgctcgagc tcgcggccgg gaatgatgcc gcgataagcg tcgcgccaag
     5581 ccgcgtcgtg cacatcggcg atcttggctt catcgcctgg ccgcgcggtt cggatggtga
     5641 cgagggtttc gaccatggag gcaatgttag ggcgacgcgc cgtccgaccg caagtcttga
     5701 ctttgccgga tcggcaaaag aatcagccgc gcggcgccaa gccgacgatg catggttttc
     5761 gccattttgc ggtcggaatt cgcgacgagg gatgctagga gacgaatcac tcctctcaag
     5821 ccgaatcctg agcatggacg cctgctcgcg agccttctcc ttccgtcgcg ccgcctttct
     5881 catgaccgcc atgctggcga cgctggccgg ggtcgccggc gcgcgcgccg aaacgtggcg
     5941 cgccgtctac aatgtctcgc tgatcggttt gccgatcgga acggcggacg tgaacggcca
     6001 ggtctcgccg accgcctatc gcatcgaggc gaacgccaag atcgtcggct tggcgacgat
     6061 gctctccaac gccaaaggcg ctgcgaccgg cgcgggcgcg atcgccggcg ggcgggtttt
     6121 gccggcgagc ttcgccacga ccgcggccaa ttcgaaaatg acccgaacgg tcaggatggc
     6181 gctaaacgaa ggcgtggtca ccggcgtcga tatttcgccg ccggtcgagg agaagccggg
     6241 acgcgttccg ctccgccagc aggacgagcg gggcattgtc gatccggtcg gcgcggcgat
     6301 ccttcccgtg gccgccaacc agccgctgat cggcccggcc gcctgcaatc gcagcatccc
     6361 gattttcgac ggctatacgc gattcgacct gcgcctgacc tatgtcggcc agaggcgggt
     6421 cgccgttaaa ggctatagcg ggccggtcgt cgtctgctcg gcccgctacg tgccgatcgc
     6481 cggccacaat cccgaccggc cggcggtcaa gttcatgatc aataacaagc agatggaagt
     6541 ctggctggcg ccgatcgaac cggcgcatgt cctggcgccg ttccgcgcct cggtgggcac
     6601 gatgatcggc accctcgtcg tcgaagcttc ggaattcagg ctcgacggga aataagccgc
     6661 atcaattcaa ctcgtcgggg atttgcggga ccggcagcac gccgacgccc tcttcgacga
     6721 gcgcgcgcgc ctcttccagg gtagcctggc cgcgaatcga gcggggcggc gcgtcgccgg
     6781 cgtgaatcgc ccgcgcctcc tcgggaaagc gtcggccgac atcgtcggtc ttggtgatga
     6841 tctcctggcg caattcccgc gccaattcgc gcaggcgccg ctggcgctcg tcgaccagcg
     6901 cgacattgac cggctgcgac tcggcggcgg cgggcgcctc ggcttttttc gcgccaacca
     6961 ccgccggcgc catcaacgcc ttggcgaccc tggaggaatc gcattcgggg caagccacga
     7021 gaccgcgtcg aagcaagcga tcgaacgagt cgctgtcggg aaaccagctt tcgaaggaat
     7081 ggtctttttc gcagaccagc gcgtatttga tcattgctca gcctgccaaa agcggatcga
     7141 gctcgccagc ggcgtccagg gcgtggaggt cgtcgcagcc gccaatgtga cgctcgccga
     7201 taaaaatctg cggcacggtg cgccgcccat gcgcccgctc ggccatcgcc gcccgcgcct
     7261 tggcgtcgcc gtcgacgccg atttcgacga acggcgcatt cttccggcgg agcagcgcct
     7321 tggcggccag gcaatagggg caagtcgctg tcgtgtagat ggtgatcgga ggcattttct
     7381 tgcgagcagt caacgaattc cgccgtccta gcatgggagg cgccgcaagc tccatatagg
     7441 cgatcacgct cccgtcacaa cccgggcgaa aacgaggata tccacggagg cggcgccagc
     7501 ccgcagcaac gcgcgggttg cggcgttggc ggtggcgctc gatgtcagga catcgtcgac
     7561 cagcacgatg cgcttgtctt tgacccgcgc ctcggcgccg gagagaacgc ggaacgcccc
     7621 ctggacattc tcggcgcgct gcgcccggct gaggccgacc tgggccttgg tcgccttgac
     7681 gcgggcaagc agggtaagat cgcaaggctt tcccgcctgc tcgccaatcg cggcggcgag
     7741 gagcgcggcc tgattgaagc ggcgcgcaaa cagccgcagc ggatgaagcg gcaccggcgt
     7801 cagaatgtcg gcctcaacga gaatgtcggc gccggcccgc gccatccagc ggccgatcgg
     7861 ccgggcgagt tcgatccggt cggaatattt gaggcgatgg acgagacggc ggaccggacc
     7921 gtcctcgaaa cgcgccgccg cccgcgcccg gccataggcc ggcggatcgg ccatggcctg
     7981 cggcgaaatc aggccggggc cgagatcctg ctcgaacggc gtgccgagcc gctcgcaaaa
     8041 cggacgctcg atgaaacgca tttcgcgcca gcagcgcggg cagagcgcgc ccgattcggc
     8101 gaccgcggcg cgacaagcaa ggcaggtcgg cggatagacg aagtccagcg ccgcgcggcc
     8161 gagcgccgct gcggcgctaa gcgcccgcct cggccaaagc ggcgcggcgt gagggccggc
     8221 atcgaaagaa agccccagca tgagcggagg ctagcgcggc cgccggcttt gcgcgagagc
     8281 ctggcggagg ttaagccacc gccatggccg cgcctgaaat cttcgacgct tcgctcgccc
     8341 gcgcccggct ggagcgcgcg gcgcggcgcg gcgcggcgga cgccgatttc ctgttgcgcc
     8401 gggcggcggc ggatttcgcc gagcgcctgg cgttggtcaa gcggcggttc gcggtggcgg
     8461 tcgatatcgc gaccccgggc ccgcacggcg ccgaggcgct gatccgcgat ccgaaaatcg
     8521 gcttcgccgc gcgcgccgcg ccgagtgcgc ccggccctgc tcggcgcgcc gctccgtcct
     8581 tcgtcggcga cgtcgagcgc ctggcgctgg cgccgcgaag ctgcgacctc gtcagctcgc
     8641 ttctcgccct gcaaggcgtc aacgacctgc cgggaacgct catccaaatc cggcaggcgc
     8701 tgagaccgga cgggctgctc cttgcatgcc tgctcggcgg ggacacgctg accgaactgc
     8761 gccagagcct gaccgccggc gaagcggaaa tcctcgccgg cgcggcgccg cgggtcgcgc
     8821 cctttgccga tgtgcgggcg atgggcgcat tgctgcagcg ggccggtttc gcgctgccgg
     8881 tggtcgatct cgagcgggtc gtcgtccgtt atccggatat gatcgcgctg atgcgcgatc
     8941 tgcgcgccat gggcgcgacc aacgtcctga tcgcccgcag ccggcgcttc ctgcgtcgcg
     9001 acgtgctggc gcgggccggc caaatctacg ccgagcgctt cgccgatccg gacggccgcc
     9061 tgcgcgcgac gttcgatctg atctggctgt cgggttgggc gccccatgaa agccagcagc
     9121 agccgctgaa gccgggatcg gccacgtcgc ggctcgaaga cgcggtgaag gcggcgcgca
     9181 aaggttcgtg acggcttcga gaaattaaag cgtcggcggg ctcagcgatt cagcgcgtgc
     9241 gcggcgtctg gcccctttcc ccgtgtagcg aagcggaacg gggagaggta aggtgagagg
     9301 cgggtcggtg tccggcctca gaatttacgc tccaagcccg tcaccgtacc tctccccgag
     9361 aggggtctcg acgagaaggc gtttgcgact ccgtggatgc ttcaacatag atcctcccac
     9421 tcttccggca gccttgcgcc gcttttcaaa aactttgcgg cgcgaacgct cgaagcgctg
     9481 agattctact cgcgtcttcc cgtaccggct ttcggcttcg agcgcgcgcc tttcgccgta
     9541 ccggatctga agcgccttgc tccttacgcg ccgctggcgg gggcgatcat cgcggcctgc
     9601 ggcgcgttcg tgctttggat cgcccaagcc ctcgccttgc cgcccctcgt cgccgccgcg
     9661 ctcgccttga gcgcgctgat catggccgcg ggcggcctgc atgaggacgg tctcgcggat
     9721 gtggccgacg gctttggcgg cggggcgacc cgcgagcgca agctcgacat catgcgcgac
     9781 agccgcgtcg gcgcctatgg cgccgccgcg ctcgtccttt cgctgctgct gcgcgccgcg
     9841 gcgcttgccg cgctgatcgg ggagagcgcc gcgatcggcg ctgcagcgct tcttctcgcc
     9901 ggcgccctct cccgcgcctg cagcctggcg ccgctcgcgc tgctcgaccc ggcgcggccc
     9961 gacggggccg gggccagcgt cggccgcctc ggcgcggatg ctttcggcaa gagcattgcg
    10021 gcctcgctcg cccttgccgc gattcttggc ctcggcgttc tcgggctcgg ccgcgccctc
    10081 cttgccttcg tgctggcctg ggccgccgcc ttggcgatcg tcgccatcgc ccggcgacag
    10141 atcggcggcc aaacgggcga tgtcggcggc gccgcgcaac aacttgccga gatcgcctgc
    10201 ctcatcggcc ttctcatcgg ggcgggcgcc gcctaactaa aaagcatgcc cagcccgtcc
    10261 accccatgtc tgaaaatctg cgtgctcgat ccgctctcgg gcctgtgcat cggttgcggc
    10321 cgcacggccg aggagatcgc cgcctggccg gcgttgagcg agaccgagcg gctcgccgtc
    10381 atggccgggc tggaggcgcg gctccgcggc gcccgctcgc gcaaggcgcg cggcggcaga
    10441 gttgcgacgc gggagcgcgg ctgatgcgct atcttctgcc gatcgggatt ctggttctcg
    10501 ccgggctgct cctgctggcg gttccggccg agacgccgct tctcggcctg gatcacaagc
    10561 gtttcgcctt cgccgcgctc ggcgccgcgc tgttcaccgc tttcctttcc cgcatcggct
    10621 cgcggcgcgt cgccctgggc gatctcgcgc gcgccttggg cggcgcgctg atctggcttg
    10681 tcctgattgt cggcctgacc ggggtctacg cctatcgctt cgaattcggc gatatcgccg
    10741 accgggtcat cgcggaactc tcgccggggg acgtcacgat cggcaaaggc ggcgaagtga
    10801 tcgttccgcg ccgccttggc ggccagttca tcatcgccgc caaggtcgac aatgttccga
    10861 cctcatttct cttcgacacc ggcgcctcga ccgtggtcct gcgcgccgag gacgccagga
    10921 aaatgggcgt cgatgtagcc aatctcgact acgacgtcga cgtgaccacg gccaatggcg
    10981 cgactcgcgc cgccgaagcg gtgctcgaca gaatcgccat cggcccgatc gtggtgcgca
    11041 acgtccatgc gctgatcgcc cgccccggcg cgctcaacga aaacctgctc ggcgcgaatt
    11101 ttctcgaccg gctgcagagc tattcggtcg aacggggacg cctggtgctg aaggggcggt
    11161 aagcaacccc ttctcccgca aagcggcgag aaggactcgt acttaccgca caatcgcgca
    11221 gctatgatcg agcagatcct cgccgtgcgc catgtcgccc gccgatggat cggcatggtc
    11281 ggcgagcagg acgaagccgt cctggtcctt aaaacgcgcg ccgatcacgg ccagggtttc
    11341 aagcgccata tgcgtcgccg cgcccggatc gccatcggca aggaggcgga acggatcggc
    11401 gtcggagaga tcaaacgaat cgagccgccg cgcgacgtag cgccggcggg caagcaggac
    11461 agaaagcggc cgccactcgg cggtgagctc gtccaggcgg gcgtcgagcg cggcgcgcgt
    11521 ttgcggcgcg acgcaatcga tgtgaatgtg aagctggtcc tggctgcgcg ccagcgagga
    11581 attgatcgcc atgccgaccg cctcgcgcgg caaaggccgg ccgagaagct tctccatcgc
    11641 gccgcgcgcg gcccaggcgg cgcgccaata gttcggcgca tcggcatcga ggagctccgg
    11701 cgactcgatc ccggccagcc gccgcgtcgg gatggcgagg aactgcgcga cgccgacaag
    11761 gtccttcagg atggcggcgc ccctttcctc gccggccgag agatcgaccg ccgcgcaggg
    11821 cgcgggcgag ccgttggcct tttcgtcggg aacacatttg tcgtggacga tccgccacaa
    11881 agccaagcgg tcgccagcgc gagtggcgac gatcgacgcg gcgacgacga aagccgcgaa
    11941 caaggcgaaa cagaagccgc gccagccaag caagagcgtc ggcatcgggc ttctaaagat
    12001 ggatcgtacg tttggcgacg ccgagcgccg cctccttgac cgcctccgac agggtcggat
    12061 gggcgtggca ggtccgcgcc aagtcttccg ccgagccgcc gaactccatc agcaccgccg
    12121 cctcgtggat catctcgccg gcgccgaagc ccatgatgtg cgcgccgagc acgcggtcgg
    12181 tcgccgcgtc ggcgaggatt ttgacgaacc cgtctgtctt gcgcatcgag cgggcgcggc
    12241 cattggccga gaacggaaac ttgccgatat tgtaggcgat cccggcgctt ttcagctcct
    12301 cctcggtctt gccgaccgag gcgatttcgg ggctcgtata aacgacgctt gggatcacct
    12361 cgtaattcac atggccgtgc tggcccgcga gaatctcggc gacggccacg ccttcgtcct
    12421 cgcctttatg ggcgagcatc gggccgcgca cgacatcgcc gatcgcgtag ataccctcga
    12481 cattggtgcg gaaattttga tcgatcgcga cttggccccg ctcggttttg acgccgaccg
    12541 cctccaagcc gagcccgtcc gtataaggcc gtcggccgat cgcgatcagc gccacatccg
    12601 tctccagcgt gacggcttcg ccgccttcgg cgggctcgat cgtcgccttc aggcccgcgc
    12661 cgcctttatc gatcttcgtg actttatggc cgagacggaa tttgaagccc tgcttttcga
    12721 gcaggcg
//
LOCUS       DATGJW010000205         7030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1075368, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000205 DATGJW010000000
VERSION     DATGJW010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7030
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1075368"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..717)
                     /locus_tag="VKU03_06425"
     CDS             complement(<1..717)
                     /locus_tag="VKU03_06425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014989319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ70934.1"
                     /translation="MNKVRLGRTDLMVTPICFGASGLGDMPDTYGYSVEADRAKATVR
                     AIFDGPANFIDTSRIYGMGRSEERIGAVIRERGLPAGFVVSTKLDRDPETNIFDAAQA
                     RRSLEQSLKALGLERIDLLHLHDPEHARSFAEATSRDGALGELFRLKEQGLAKAVGLA
                     AGPVTMMIPLLRDWDFDALITHNRFTLVNRSAEDMLNFAASKGIAVLNAAPYAGGVLA
                     KGSQSYRRYVYQEASDAMLDP"
     gene            complement(789..862)
                     /locus_tag="VKU03_06430"
     tRNA            complement(789..862)
                     /locus_tag="VKU03_06430"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(828..830),aa:Gly,seq:tcc)
     gene            933..1532
                     /locus_tag="VKU03_06435"
     CDS             933..1532
                     /locus_tag="VKU03_06435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLJ70935.1"
                     /translation="MTKNDEDRRAIGELIDNWIVWRDAGDWDRFRSVWHDDGRMMATW
                     FQGSCDDFIRVSREGWEKGVSILHFQGGSSIEIDGLRAVAQTKMTISQRAKAEGIVCD
                     VVCTGRFYDFLEKRGGRWGLVLRQPIYEKDRLDPVDPSAVLRLDADLLDRFPEGYRHL
                     AYVQSQIGYKVKPDMPGLKGPEVEALYRRGADWLSGRPI"
     gene            1655..2812
                     /locus_tag="VKU03_06440"
     CDS             1655..2812
                     /locus_tag="VKU03_06440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0042597 - periplasmic space [Evidence
                     IEA];
                     GO_function: GO:0015159 - polysaccharide transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis/export family
                     protein"
                     /protein_id="HLJ70936.1"
                     /translation="MRIIYAPALALALLASGCSSVPTEGPSASDIVAERSAPVQRFEL
                     VDLDMNVAQVLAGRRADKTIASFGDYRPSSQLRIGIGDQVTVTIWEAAAGGLFSAPLL
                     TDKFSTGSKSATIPPQIVDRDGMITVPYAGQVHVAGHTPKEVQAAVEQALEGKAIQPQ
                     VLVDVTRSPANTASVLGEVVQGARVPLSVKGDRILDVIAEAGGVKAPVSETFVELTRG
                     KRTARVALSRVVADHREDIYVQPGDVITVVRDPQTFIAYGATGRNAEIPFDAEGINLN
                     QAIAKAAGLLDYRADPEGVFLFRFEPESVARVLRPNSPLVQPGKLTPFVYRLNLRDAN
                     SLFVAQRFPIFNKDVIYVSNAPLSDVQKVMEIFNLAVTPAASAASIYSVTK"
     gene            complement(2992..4467)
                     /locus_tag="VKU03_06445"
     CDS             complement(2992..4467)
                     /locus_tag="VKU03_06445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ70937.1"
                     /translation="MAKRPERTTIGGDLAISRLICGLWQVADLEKDGATLDRDAAADA
                     LDAYARAGFDTFDMADHYGSAELIAGRLLARYPPGEERPLAFTKWCPPPGPMTPDVVR
                     RGIDERRERLGVERIDLLQFHWWTFEHPAWLDALHELAKLKEEGAIRAVGVTNFDAAH
                     LALALADGAPIVSNQVSFSLIDRRAAGPLSELCRRAGVRLLAYGTLCGGFLSEAWLDR
                     PAPQSLPDWSRSKYKRFIDAAGGWPALQAILKAAHGVAKKHGVSIANVATRWVLDHDA
                     VAAIIIGARIGEREHRGDNLKAFAFALDAEDRALLEEAFGATQAIPGDCGDEYRRPPY
                     LTASGDLSHHLDAAPAAYRAEPAPARPDRLRVSSGSAFEPIAGYSRAVRVKDRIFISG
                     TTATHGADRCVAPGDPGAQTTYILDKIAAALAALGGAIEDVVRTRVYLRDAAQWEPVS
                     RAHGRVFAGVLPANTLVQAGALIGDYEVEIEAEAIVPDERR"
     gene            complement(4451..5464)
                     /locus_tag="VKU03_06450"
     CDS             complement(4451..5464)
                     /locus_tag="VKU03_06450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006938948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70938.1"
                     /translation="MILDLFGYGAFFLTMALTYAIICLGLNVQWGQTGLFNVGVAGFV
                     AIGAYVSALLTTPDTPGRLGGFGLPIAVGWLGGACAAGLVSFLVGALTIRLRADYLAI
                     ATFGAAVVIHLCALNLQRVTGGPFGIGFIPRPFASAAGDPLLFGLLNLALLAGVALVL
                     YLALERLLRSPWGRVLRAIREDETAALALGKNSVHFRLQAFAIGGAVMGLAGAAQAHF
                     IGFIAPDNYQPTLTFQVWAMLIVGGSGNNRGAILGAVVVWGVWAASASAIAAIFPPEQ
                     QARAASLQIVLIGLALCAILLLRPRGILGEVKVVSRHLAGLPAAAQLPSKVHFEALDG
                     EET"
     gene            complement(5461..6372)
                     /locus_tag="VKU03_06455"
     CDS             complement(5461..6372)
                     /locus_tag="VKU03_06455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018896736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ70939.1"
                     /translation="MSLQVVIDGLIAGAMIGLGAIGVTLTYSILRFANFAHGEFIAWG
                     AYFALAVSGFLGAAAPGLAAPIGPFSFGWSLPIAACAAIVLTAALALLVDTLLFGRLR
                     AKGGAVIILVMASFGAALTLRNLLEFLFTSRPAYYVEGLQIALRFGRVRATPDALLCL
                     ALAGALVVAIHLLIARTPIGRAMRAVSENPQLAGLVGVDSRKVVRLVWILGAGLAAIA
                     GIMAGLLVQIRPTMGLDLLLPLFAAAILGGIGSAPGALIGGLIVGLAESATVSLIGAE
                     WRAAVAFVILVAILLVRPRGLFGRSPP"
     gene            complement(6369..>7030)
                     /locus_tag="VKU03_06460"
     CDS             complement(6369..>7030)
                     /locus_tag="VKU03_06460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018896737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70940.1"
                     /translation="RGVNFSVRAGEFVVLLGPNGAGKSTLVKAVAGLAPIHAGAVFFN
                     GEDVTGAPAHQKIRRGLAFVPQTDNVFATLTIEENLRLAADILPKPKRARRIAELYAA
                     FPDLALRPGRRAGQLSGGQRQMLAAARALIVEPSLLILDEPSAGLSPKIAAEVFARLK
                     TINQNGVAVVLVEQNVKAALAVADRAVILVEGEARHEGKAADLADDPLVGRLYLGAAR
                     R"
BASE COUNT         1228 a         2276 c         2483 g         1043 t
ORIGIN      
        1 cggatcgagc atggcgtcgg acgcctcctg atagacatag cgccggtagg attgcgagcc
       61 cttggccagc acgccgccgg catagggcgc ggcgttcagc acggcgattc ctttcgaagc
      121 ggcgaaattc agcatgtcct cggcgctgcg attgaccagc gtgaaccgat tgtgggtgat
      181 cagcgcgtcg aaatcccagt cgcgcagaag cgggatcatc atcgtcacgg ggccggcggc
      241 gaggccaacc gctttggcca atccctgctc cttgaggcga aacagctcgc ccaaagcgcc
      301 gtcgcggctt gtcgcctcgg cgaacgaacg ggcatgctcg ggatcgtgca ggtgaagcag
      361 gtcgatccgt tcgagcccga gcgccttgag gctttgttcg agcgagcggc gcgcctgcgc
      421 ggcgtcgaat atattggttt caggatcgcg atcgagcttg gtcgagacga cgaagccggc
      481 gggcaggccg cgctcgcgga tgaccgcgcc gatccgctcc tcgctgcggc ccatgccgta
      541 aatgcgcgac gtatcgatga aattcgccgg cccgtcgaaa atcgcccgca ccgtggcttt
      601 ggcgcggtcg gcttcgacgc tgtagccata ggtgtcgggc atgtcgccga ggcctgacgc
      661 gccgaagcag atcggcgtga ccatcaggtc cgttcggccc aagcggactt tgttcatggc
      721 gtcctttctg ccggcgcttg cgcgagccta cgcctgacgc gccgaacgcg gcctgatctg
      781 gattaagctg gagcgggtga agggaatcga accctcgtat tcagcttgga aggctgctgc
      841 tctaccattg agctacaccc gcatggcgtc tccttagcat ggggagccgc ggcggcggaa
      901 gcggccggtt tgcggcgcga tgaggagagg cgatgacgaa aaacgacgag gaccggcgcg
      961 cgatcgggga gctgatcgac aattggatcg tctggcgcga cgccggcgat tgggatcgct
     1021 tccgctcggt ttggcacgat gacggccgga tgatggcgac ctggtttcag ggaagctgcg
     1081 acgatttcat ccgcgtcagc cgcgagggct gggagaaggg cgtcagcatc ctccacttcc
     1141 agggcggctc gtcgatcgag atcgacggct tgcgcgccgt cgcgcagacc aagatgacga
     1201 tttcgcagcg cgccaaggcg gaggggatcg tctgcgacgt cgtttgcacc ggccgctttt
     1261 acgatttcct cgagaagcgc ggcggccgct ggggcctcgt cctgcgccag ccgatctacg
     1321 agaaggatcg cctcgacccg gtcgatccaa gcgccgtttt gcgcctcgac gccgatttgc
     1381 tcgaccgctt ccccgagggt tatcgccatc tcgcctatgt gcaaagccag atcggctaca
     1441 aggtcaaacc ggacatgccc gggctcaagg ggccggaagt cgaggctctc tatcggcgcg
     1501 gcgcggattg gctgagcggt cgcccaatat agccgcgagc tgtggccctg cggccacggg
     1561 ggctttgaaa tcgttcggtt ttgagcggct tcaatggaaa ttaacggggc gggctgctaa
     1621 tggccctggg tgcgttggag ggcgggccat cacgatgcgg atcatctatg cgccggccct
     1681 ggcccttgct ctgctggcgt ccggctgctc gtcggtgccg accgaggggc cgtcggcctc
     1741 cgacatcgtg gcggagcgct cggccccggt ccagcgcttc gaactcgtcg atctcgacat
     1801 gaacgtcgcc caggtcctgg ccgggcggcg ggcggacaag acgatcgcca gtttcggcga
     1861 ttaccggccg tcgagccaat tgcgcatcgg gatcggcgac caggtgacgg tgacgatctg
     1921 ggaggcggcg gcgggcggcc tgttctcggc cccgttgctc accgacaaat tctcgaccgg
     1981 ctcgaaaagc gcgaccatcc cgccgcagat cgtcgaccgc gacggcatga tcaccgtgcc
     2041 ctatgccggc caggtccatg tcgccggcca tacgccgaaa gaggtgcagg cggcggtcga
     2101 gcaggccttg gaaggcaagg cgatccagcc gcaggtcctg gtcgacgtga cgcgctcgcc
     2161 ggccaatacg gcgagcgtgc tcggcgaggt ggtgcagggg gcgcgggtgc cgctctcggt
     2221 caagggcgac cgcattctcg acgtgatcgc cgaagcgggc ggggtcaagg cgccggtcag
     2281 cgagaccttc gtcgaactga cccgcggcaa gcgcacggcg cgggtggcct taagccgggt
     2341 ggtcgccgac caccgcgagg acatctatgt gcagccgggc gacgtgatca cggtggtgcg
     2401 cgatccgcag accttcatcg cctatggcgc gaccggccgg aacgcggaga tcccgttcga
     2461 cgcggagggg atcaatctca accaggcgat cgccaaggcg gcgggcctgc tcgactatcg
     2521 cgccgatccg gagggcgtgt tcctgttccg cttcgagccg gaaagcgtgg cgcgggtttt
     2581 gcggccgaat tcgccgctgg tccagccggg caagctgacg ccgttcgtct accggctgaa
     2641 ccttcgcgat gcgaactcgc tgttcgtggc gcagcgcttc ccgatcttca acaaggacgt
     2701 gatctacgtc tcgaacgcgc cgctgagcga cgtgcagaag gtgatggaga tcttcaacct
     2761 ggcggtgacg ccggccgcct cggcggcgtc gatctatagc gtgacgaagt agcggggggc
     2821 gttatcctcc cccgtttcgc gaagcggaac gggggagggg gaccatgcga agcatggtgg
     2881 agggggccgc gtggttggga attatcctga gagaccgagg ccccctccac cgcgctgacg
     2941 cgcggtcccc ctcccccgtc tttcgacggg ggaggatagc tccttgacga gtcatctcct
     3001 ctcatccggc acgatcgcct ccgcctcgat ctcgacctcg taatccccga tcagcgcgcc
     3061 ggcttgcacc agcgtgttgg ccggcaggac gccggcgaag acgcggccat gggcgcgcga
     3121 caccggctcc cattgcgccg cgtcgcggag atagacgcgg gtgcgcacaa cgtcctcgat
     3181 cgcgccgccg agcgcggcca gcgcggcggc gatcttgtcg agaatgtacg tcgtctgggc
     3241 gcccgggtcg cccggcgcga cgcagcggtc ggcgccatgc gtcgccgtcg tgccggagat
     3301 gaaaatcctg tccttcacgc gtacggcgcg gctatagccg gcgatcggct cgaaggcgct
     3361 gcccgaagaa acgcgcagcc ggtctggccg ggcgggcgcg ggctcggcgc ggtacgcggc
     3421 cggggcggcg tcgagatgat ggctgagatc gcccgaagcg gtcagataag gcggccggcg
     3481 atattcgtcg ccgcaatcgc cgggaatcgc ttgcgtcgcg ccgaaggctt cctccagcag
     3541 cgcccggtct tcggcgtcga gcgcgaaggc gaaagcttta agattgtcgc cgcggtgctc
     3601 gcgctcgccg atccgcgcgc cgatgatgat cgcggcgacg gcgtcatggt cgagaaccca
     3661 gcgcgtcgcg acattggcga tcgagacgcc gtgtttcttc gcgacgccat gcgccgcctt
     3721 caggatcgcc tgcaaagccg gccagccgcc ggccgcatcg atgaagcgct tgtatttcga
     3781 gcggctccaa tccggcaggg attgcggcgc cggccggtcg agccaggcct cggaaagaaa
     3841 gccgccgcac agcgtcccat aggcgagaag cctcacgccc gcgcggcggc agagctcgga
     3901 caacggcccg gcggcgcggc ggtcgatcag cgagaaggag acctggttgg agacgatcgg
     3961 cgcgccgtcg gccaaagcga gcgccagatg ggcggcgtcg aaattggtca cgccgaccgc
     4021 ccggatcgcg ccttcctcct tgagcttggc gagttcgtgc agggcgtcga gccaggccgg
     4081 atgctcgaaa gtccaccaat ggaattgcag aaggtcgatt cgctcgacgc cgagccgctc
     4141 gcgccgctcg tcgatcccgc gacgcacgac gtccggcgtc atcggcccgg gcggcgggca
     4201 ccatttggtg aaagcgagcg gccgctcctc gcccggcgga taacgcgcca gcaggcgccc
     4261 ggcgatcagc tcggcgctgc cgtaatgatc ggccatgtcg aacgtatcga agccggctct
     4321 ggcgtaagcg tcgagcgcgt cggcggcggc gtcgcggtcg agcgtcgcgc cgtccttttc
     4381 gagatcggcc acttgccaga ggccgcagat caggcggctt atcgcgagat cgccgccgat
     4441 cgtggtccgt tcaggtctct tcgccatcga gcgcctcgaa atgaacttta gaggggagtt
     4501 gcgcggccgc cggaaggccg gcgaggtggc gcgaaaccac cttgacctcg ccgagaatgc
     4561 cgcgcggccg cagcaagagg atcgcgcaaa gcgcgaggcc gatcaggacg atctgcagcg
     4621 aggcggcgcg cgcctgctgc tcgggcggga agatcgccgc gatggcgctg gccgacgccg
     4681 cccagacgcc ccaaacgacg accgcgccca aaatcgcgcc gcgattgttg cccgagccgc
     4741 cgacgatcag catcgcccag acctggaaag ttagagtcgg ctgataattg tcaggcgcga
     4801 tgaagccgat gaaatgggcc tgcgccgcgc cggcgaggcc catcacggcg ccgccgatgg
     4861 cgaaagcttg caagcggaaa tgaaccgaat tcttgccgag cgccagagcc gccgtctcgt
     4921 cctcgcggat cgcccgcaat acccggcccc atgggctgcg cagcagccgc tcgagcgcga
     4981 gatagagtac gagggcgacg ccggcgagca gcgcgaggtt gagcagccca aacagcagcg
     5041 ggtcgccggc ggcggacgcg aaagggcggg ggatgaagcc gatcccgaac gggccgccgg
     5101 tcacccgctg caaattgaga gcgcagagat ggatgacgac ggcggcgccg aacgtggcga
     5161 tcgccagata gtcggcccgc agcctgatcg tcagcgcgcc gacgaggaag ctgacgaggc
     5221 cggcggcgca agcgccgccg agccagccga cggcgatcgg caggccgaag ccgcccagtc
     5281 ggccgggcgt atcgggcgtc gtcagcagag ccgagacata ggcgccgatc gcgacgaatc
     5341 cggcgacgcc gacattgaac agaccggtct ggccccattg cacgttgagg ccgaggcaga
     5401 tgatcgcgta ggtcagcgcc atcgtcagga agaacgcgcc atagccgaag agatcgagga
     5461 tcacggcggc gatctcccga acaggccgcg cgggcgcacg agcaggatgg cgaccaggat
     5521 cacgaaagcg accgcggcgc gccattcggc gccgatcagg ctgacggtcg ccgattcggc
     5581 gagtccgacg atcaggccgc cgatgagcgc gccgggcgcg ctgccgatgc cgccgagaat
     5641 cgccgcggcg aaaagcggca gcaacagatc gagtcccatt gtcgggcgga tttgcaccaa
     5701 taaaccggcc atgatcccgg cgatcgccgc aaggcccgcg ccgagaatcc aaaccaggcg
     5761 aacgaccttg cggctgtcga cgccgaccaa cccggcaagc tgcgggtttt cactcaccgc
     5821 gcgcatggcg cggccgatcg gcgtgcgcgc gatcaggaga tggatggcga cgaccagggc
     5881 gccggcgagc gcgagacaga gcagcgcgtc gggcgtcgcg cgcacgcggc cgaaccgcaa
     5941 agcgatctgc aatccctcga cataataagc gggccgcgag gtgaagagga attcgagcag
     6001 gttgcgcaaa gtgagcgccg cgccgaagct cgccatgacc aggatgatca ccgcgccgcc
     6061 tttggcgcgc aaccggccga acagcagcgt atcgaccagc agcgccagcg ccgcggtcag
     6121 tacgatggcc gcgcaggcgg cgatcggcaa cgaccagccg aacgagaacg ggccgatcgg
     6181 cgcggcaagg cccggcgccg cggcgccgag aaagccgctg acggcgaggg cgaagtaagc
     6241 gccccaggcg atgaattcgc catgcgcgaa attggcgaag cgcaggatcg aataggtcag
     6301 ggtgacgccg atcgcgccga ggccgatcat cgcgccggcg atcagcccgt cgatgacgac
     6361 ttgcaggctc atcgccgcgc cgcgccgaga tagagccggc cgaccagcgg atcgtcggcc
     6421 agatccgccg cttttccctc gtgacgcgcc tcgccttcga cgaggatcac cgcccggtcg
     6481 gcgacggcga gcgccgcctt gacattctgc tcgacgagaa cgaccgcgac gccgttttga
     6541 ttgatcgtct tgagccgggc gaagacctcg gcggcgattt tcggcgaaag gccggcggag
     6601 ggctcgtcga gaatgagcag cgacggctcg acgatcagcg cccgcgccgc cgccagcatc
     6661 tggcgctggc cgcccgacaa ttgtccggcg cggcggccgg ggcgcaaggc gagatcgggg
     6721 aaagccgcgt agagctcggc gatgcgccgc gcccgttttg gcttcggcag aatgtcggcg
     6781 gcgaggcgca gattctcctc gatcgtcagg gttgcgaaaa cgttatccgt ctgcggcacg
     6841 aaagcgaggc cgcggcggat tttttgatgg gccggcgcgc cggtcacgtc ctcgccgttg
     6901 aagaagacgg cgccggcatg gatcggcgcg aggccggcga ccgccttcac cagggtcgat
     6961 ttgcccgcgc cgttcgggcc gagcagaacg acgaattcgc cggcgcggac gctgaaattc
     7021 acgccgcgca
//
LOCUS       DATGJW010000206         9560 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1282906, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000206 DATGJW010000000
VERSION     DATGJW010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9560)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9560)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9560
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1282906"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            241..456
                     /locus_tag="VKU03_06465"
     CDS             241..456
                     /locus_tag="VKU03_06465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70941.1"
                     /translation="MPYSVGWGLQEGVFGRKCETAREALALAEEEIAAGRSDVIVTDL
                     VTQEPVPIEALRERAEEEAGEEGARST"
     gene            complement(748..1017)
                     /locus_tag="VKU03_06470"
     CDS             complement(748..1017)
                     /locus_tag="VKU03_06470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70942.1"
                     /translation="MSQASNIRSWAPEDILVEPMLTDGEARSVLRRQLIGSVVAAMVV
                     ILFAGFTAIRSGHEAAAESSPHRFAVVQQPSFVAPAGKVIALKRY"
     gene            1420..2001
                     /locus_tag="VKU03_06475"
     CDS             1420..2001
                     /locus_tag="VKU03_06475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70943.1"
                     /translation="MMTAAFRALCLGPLAALALGLMIENCNVQTAAGAAAAAGGDGPS
                     GGAGSSSGGNGGPHFPTAVAAPDRGPPDVKPITRSTEAAISQCAEDDRICIADALDAY
                     AAALRKLSPPLPPELQSLPDIVSRAAHRVRQAKTRAQAAKAIKVAIAEVRKTISLLKA
                     DDPVVLKAETRQGTFVAETLAVAENKLEKASGL"
     gene            2124..4004
                     /locus_tag="VKU03_06480"
     CDS             2124..4004
                     /locus_tag="VKU03_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HLJ70944.1"
                     /translation="MIAGRAGAETRVALVIGNAQYRHLPALANPGFDAKDIGADLQTL
                     GFKVSLGIDLDLAGMERAISAFAKAAAEADVSLVYYSGHGVQVAGHNYLIPVDAELHD
                     EQDIYKRTVHFDDVMKALQQAKGLHLLFLDACRTNPVKDASPVAHAQGLARVGDAANF
                     LIAFATQPDNVAYDGAGRNSPFAQALLSHLSTTGQDVSSMMIEVRKDVIATTGGAQIP
                     WENSSLTRQFYFAPGEVADGSSETLLWRLGAGQKDASLLRIYLDRYPEGAHVGDAKAL
                     LAEIGRQGKPNPAPAKNENVEDLLWRLAKSERQRPLLELYLARYPSGAHRPEAEALIE
                     RLPDSEGAAPPAVLCQRLATHPRDATADYPGVELETLARNADLAMSACREAAAQHPGE
                     AHYVALLARATAASGKRDEAIGLYQQAAEAGDARAMVSLGLLYEAGDGLPKDLKAADE
                     LYAKAASRGSADGAINLAVALMKGAGIERDTARAVSLLKTAAAGGSAIATYDLGVLAQ
                     QGVAGDADEALDFFRRSAALGDPRGHVAAAILLDEGRGVAKNPEAAADQLLRGVAADS
                     GEAFSQLTAKAPAWSRETIKAVEARLKSAGYYSGPVDGRGGAALAPALKQWRLLGPPQ
                     KS"
     gene            complement(4228..5169)
                     /locus_tag="VKU03_06485"
     CDS             complement(4228..5169)
                     /locus_tag="VKU03_06485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70945.1"
                     /translation="MAGQMKAFASRSDRRRRAPFVAWSVLMALLLGLAAGRAEATPAR
                     LASDADIAGFAALVKAYSGASRVGWAIAAPSLAEADAVIANIGLHLAPADQALLQRIK
                     PHSAAEAGNVAEAARGPVGWLEPQLTRPTDSPACSWQVWLADPGAPTPGQGPANIPLA
                     PNDRAPASAAATFRIAFAGLLQSKIYAFGETRPGAIRDLAIAPDVNIPVAAGETETIL
                     LAISRQPAPFLESIRAALASSGGQRVDLGKDHALGENLLGRGRGIGANIQLVGPNMVV
                     AKGQSAKKTVVASADAHSGELTETCTFVLTPAPEAMR"
     gene            complement(5184..6539)
                     /locus_tag="VKU03_06490"
     CDS             complement(5184..6539)
                     /locus_tag="VKU03_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70946.1"
                     /translation="MSARVFVPLVGANGEAAQVFEAIAGDVPPDAHPARLLPQISELF
                     DFGLYGIGQTVREAVTRFIDARREKLWLGRRNGLVNPWNLRISDLQVGRLSDENARSA
                     GLGLTLAAILQAFGRDPGIVFATGEIRLPASPGELTVGVGPVDGLRGKLLLVGDYVVQ
                     HRKALEGAKMTVVLPTTTPDGRPLSLAEASVLERLKREAGEAGAKIEFVFVDTLEGVE
                     QALGPFALREIVTPARALWAIAACAALALVVGGWVAWRNAPIELAWRPVTIAEGATRS
                     LAAPAPEATPQRARYDAASDKFQLLGPCYDAQRQPLVIGGETLLLRVGARDGLPFASR
                     LRPPRIFIASVSRAADPVILDANQFRSLLPASRTDDLIDLTMAVPVEPVEDEVRLFVV
                     ATRDPSVEITALQDDLRKRLKGLSGAAVLTTTTSFLEDRLGAEIQYQFKVVNDPGQCP
                     A"
     gene            complement(6536..6976)
                     /locus_tag="VKU03_06495"
     CDS             complement(6536..6976)
                     /locus_tag="VKU03_06495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70947.1"
                     /translation="MAGEFTDAQAARFLRTLRVATQTALAEYALSGGRAASRPRLAFM
                     ALAAKGPGEWRFPASGLLSADGLVLAKLVSEPAAEPVLELQAQGVAGLSLYASRSARA
                     TFAGGRALDGAFDRDGRMRLSLAGAAIEEADLSSFELELLDEAP"
     gene            complement(7038..8114)
                     /locus_tag="VKU03_06500"
     CDS             complement(7038..8114)
                     /locus_tag="VKU03_06500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70948.1"
                     /translation="MSASTSAKGGRDVVAYFAGHLANLVNGNDSLSDADETVSLKPFS
                     LYEKVHRSLVANRKYSEVLVGVWAYPPPNTVEDAADFYFDNVLDRPIGSRGEGPSSAD
                     NLAALVASRAVGPVAPRPKLPLSIWKALVGGPTMLRRFAEKKELFELSNRILKCLDAS
                     NRPYAEVLRLGLCPKEWLVGDEAVGVNTLKGANAFLKALKIEMKGVFGRRPSPAELEA
                     AFAQAPVPGFKDVKSFAATPLGSAILTRLAGQDETFLTSFEAVEATLSEEVEAAADEP
                     LMDAEEAAPFLEMAVKAGAIETEEKRLLVAIMAGRSLAEAMGSNLYLRRRLKADFDND
                     LEAYINDLSARTAKFIQAAGAARP"
     gene            complement(8303..9217)
                     /locus_tag="VKU03_06505"
     CDS             complement(8303..9217)
                     /locus_tag="VKU03_06505"
                     /inference="COORDINATES: protein motif:HMM:NF019846.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLJ70949.1"
                     /translation="MKYCDLRPAIAAYREGRNVMQTLRETLGETANTPQIIEIAYDLQ
                     AGGYIDHVRANRELWRAYTSELAGVLRDHIAAGDRVLEAGTGEMTTLVGVANACYAGI
                     SRHYAADISWSRIAKGRAFAAAEMRADLASAMSSFVGDLLRLPLRDHSIDVVWTSHAL
                     EPNAGREQAALAELFRVARKRVVLFEPSYEDNSAAGRARMDALGYIRGLPEAIAAQGG
                     RLEEKRPIAAISNPLNPTYAYIVTPPGGASRNDDEWACPATRLPMQKCGDCYWSEASR
                     LAYPIIGSIPVLREESAVYATSLADAGS"
     gene            complement(9332..>9560)
                     /locus_tag="VKU03_06510"
     CDS             complement(9332..>9560)
                     /locus_tag="VKU03_06510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70950.1"
                     /translation="PRESNALDICFEADAPGALDGAAFAAGRNEVRMTIDPAAVTHFA
                     ETWIIPLHFAFDAPSGSVRLLSCGLSGVPSR"
BASE COUNT         1605 a         3207 c         3247 g         1501 t
ORIGIN      
        1 taggggtcac aggttcgatc cctgtcgcgc ccaccaggta tgatcaatac cttagcaaaa
       61 tcgttatgta accggggcgg atttccgggc ggcatgtcgg cgcggtcgct gaccgcgccg
      121 cgctccgccg ccgcttttac gcgaaacgag gcgcttcgcg cccgccgcgg tcagcgaccg
      181 cggctacaat ggccggagcg cccggaaatt cgccccggtt ataaccctgg gagagggccg
      241 atgccttatt ccgttggttg gggacttcaa gagggcgtgt tcgggcgcaa atgtgagacg
      301 gcgcgcgagg cgcttgccct cgcggaggaa gagatcgctg ccggccgctc ggacgtcatc
      361 gtgaccgact tggtgacgca agagcctgtg ccgatcgagg cgctgagaga acgcgctgag
      421 gaagaggccg gcgaggaagg cgctcgctcg acctgaacgg cagccatgat cccggatcat
      481 cggcgggagg tctgacgccc gacgcgcaat gtgcttgcga ctcttggctg gccgcgggca
      541 agcgctctgc atcgcccttc gtcaaaagca tgggagatca attggaggaa ctgcgccccg
      601 tcgcgtcaaa aagctcagcc cgccgagcgc gaatcggcgg gccgacgggc agcatgaagc
      661 ggcttcgcgc gccggccgaa atcaatgctg ctcgaactgc aatagcgtgg cgaagacgga
      721 agatgtgaca agtgtcgcct ctccggctca ataacgcttc aatgcgataa cttttccggc
      781 cggcgcgacg aaactcggct gctggacgac cgcgaagcga tgcggcgatg attccgcggc
      841 ggcttcgtgt ccagaccgaa tcgccgtgaa gcccgcgaac agaatgacga ccatcgcggc
      901 gaccacggag ccgatcagtt ggcgcctcag cacggatcgc gcctcgccgt ccgtcaacat
      961 cggctcgacg agaatatcct ccggcgccca ggagcgaatg tttgaagctt ggctcatctt
     1021 tccctccatc cctcaattcg atcggtcgtt cagccgaaat gaagcagcgt gaaaacgaag
     1081 ctcgcgaaga agccgatgac gaagaggagg ttgaaagcgt tgtggctcgg aacgcgctcc
     1141 atggcgccct cctggtctgg cgatcgctcg gactcgcata agtccgtcgc gcatagagga
     1201 cttagcgcgc cttccgcgcg aacgatgtgg cgcttcgcac atggcgcgca aaaagggcgt
     1261 cccgaaaggc ggcggaccgt aatcgaagga cgcgccgcgc tatctgcgcc gcttgacgac
     1321 cgtccgagct tcagcgctct ctaagcgccg gcgcgtcgaa ttcgccgaac gagcagaggc
     1381 cgaccggatc ggcgctccct ttagttccga ggacccgcaa tgatgaccgc cgcctttcgc
     1441 gccttgtgcc ttggtccgct cgccgcgctc gcgctcggcc tgatgatcga gaattgcaac
     1501 gtgcagaccg ccgccggcgc cgcggccgcg gctgggggag acggccccag cgggggcgcc
     1561 ggctcgtcgt ccggcggcaa tggcggtccg catttcccga ccgccgtcgc cgcgccggac
     1621 cgaggccccc cggacgtcaa gccgatcacc cggtcgaccg aggccgcgat ctcgcaatgc
     1681 gcggaggacg accgcatttg catcgccgat gcgctcgacg cctacgccgc cgcgttgcgc
     1741 aaactttccc cgcccttgcc gccggaattg cagagcttgc ccgacatcgt ctcgcgcgcc
     1801 gcccatcgcg tccgccaggc caagacgcgc gcccaggcgg cgaaggcgat caaggtcgcc
     1861 atcgccgagg tgcgcaagac catctcgctg ctcaaagcgg acgatccggt cgtgctgaag
     1921 gccgagaccc ggcaagggac gttcgtcgcc gagacgcttg cggtggcgga gaataagttg
     1981 gagaaagctt ccgggctcta gtcgcgtttg aagcgaggct cgtgaggttc gatttggccc
     2041 gaacggatcg cgacccgccg gcgccgcgcc ggttacgcgg gtcgccgctt tggcgcttct
     2101 gcctgctctt aggcttatcg gtcttgatcg ccggccgcgc cggcgcggaa acccgcgtcg
     2161 cgctggtgat cggcaacgcg cagtatcggc atcttcccgc cttggccaat ccgggcttcg
     2221 acgccaagga tatcggcgcg gacctgcaaa cgctcggctt caaggtcagt ctcgggatcg
     2281 atcttgatct tgcagggatg gagcgcgcga tttctgcttt cgccaaagcg gcggcggagg
     2341 ccgacgtctc cctcgtctat tacagcggcc atggcgtgca ggtggctggc cacaactacc
     2401 tcatcccggt cgacgccgaa ttgcatgacg agcaggatat ctacaagcgg accgtccatt
     2461 tcgacgacgt gatgaaggcg ctgcagcagg ccaaggggct gcatctcctg tttctcgacg
     2521 cctgccgcac caatccggtg aaggacgctt cgcctgtcgc tcatgcgcaa ggcttggcgc
     2581 gggttggcga cgccgccaat ttcctgatcg ccttcgccac ccagcccgac aatgtcgcct
     2641 atgacggggc cggccggaac agccctttcg cgcaggctct gctcagccat ttgtcgacga
     2701 ccggccaaga cgtttccagc atgatgatcg aggtgcgcaa ggacgtcatc gcgaccaccg
     2761 gcggcgcgca gatcccctgg gaaaattcct ccttgacccg acaattctac tttgctccgg
     2821 gcgaagtcgc ggacggctcg tccgagacgc tgttgtggcg gctcggcgcc ggccagaagg
     2881 acgccagcct gcttcgcatc tatctcgacc gctatcccga aggcgcgcat gtcggcgacg
     2941 ccaaggccct gctcgccgaa atcggccgcc agggcaagcc gaaccccgcc ccggcgaaga
     3001 atgagaacgt cgaggacctg ctctggcgcc tggcgaaaag cgagcgccag cgcccgctcc
     3061 tcgaactcta cctggcgcgc tacccgagcg gcgcgcaccg cccggaggca gaggcgctga
     3121 tcgagcgcct gccggactcg gaaggcgccg cgccgccagc cgtcctctgc cagaggctgg
     3181 cgacccatcc gcgcgatgcg acggccgatt atcccggcgt cgagctcgaa accttggcgc
     3241 gcaacgccga tctcgcgatg tccgcctgcc gcgaggcggc ggcgcagcat ccgggcgaag
     3301 cgcattatgt ggcgctgctc gcccgcgcca ccgccgcttc cggcaagaga gacgaggcga
     3361 tcggactcta tcagcaagcg gccgaggccg gcgacgcgcg ggcgatggtc agtctcggcc
     3421 tgctctacga ggcgggcgac ggcctgccca aggacctcaa agccgccgac gagctttacg
     3481 cgaaagccgc ttcgcgcggc agcgccgacg gcgcgatcaa cctcgccgtc gccttgatga
     3541 agggcgccgg aatcgagcgc gacacggcgc gcgccgtgtc gcttctgaaa accgcggcgg
     3601 ccggcggttc ggcgatcgcc acctacgatc tcggcgtgct cgcccagcag ggcgtcgccg
     3661 gcgacgccga cgaggcgctg gatttcttcc gccgatcggc ggctttgggc gacccgcgcg
     3721 gccatgtcgc ggcggcgatc ctgctcgacg aggggcgcgg cgtcgccaag aacccggaag
     3781 cggcggccga ccaattgctg cgcggcgtcg ccgccgattc cggcgaggcg ttcagccagc
     3841 tcaccgcgaa agcgccggcc tggtcgcgcg agacgatcaa agcggtcgag gcgcggctga
     3901 aaagcgccgg ctattattcc ggcccggtcg acggcagagg cggcgcggct ttggcgccgg
     3961 cgctgaagca atggcgcctg ctcgggccgc cgcagaagtc gtgagcgggc gtttggggtc
     4021 gagggtatgc gcgccattcc tcccccgcaa agcgggggaa gggggaccgc gcgtcagcgc
     4081 ggtggagggg gcgcggcggt tgagaattat cctgagaggc cgcggccccc tccaccatgc
     4141 ttcgcatggt ccccctcccc cgttacgctt cgctccacgg gggaggattc tccatcccgc
     4201 tcgcctttcg tacgtagcgc ggcgatgtca ccgcatcgct tccggcgccg gcgtcagcac
     4261 gaaagtacaa gtctccgtca gctcccccga atgcgcgtcg gcgctcgcca cgaccgtctt
     4321 ctttgcgctc tgccccttcg cgacgaccat gttcgggccg acgagctgga tattggcgcc
     4381 gatgccgcgg ccgcggccga gcaggttttc gccgagcgcg tgatccttgc cgagatcgac
     4441 gcgctggccg ccgctcgatg cgagcgcggc gcgaatgctt tcgaggaacg gcgccggctg
     4501 gcgcgaaatg gcgaggagga tggtctccgt ctcgccggcc gccaccggga tgttcacgtc
     4561 gggcgcgatg gcgaggtcgc gaatggcgcc gggcctcgtc tcgccgaaag cgtagatttt
     4621 cgactgcaaa agcccggcga aggcgatcct gaacgtcgcc gcggcgctgg ccggggcgcg
     4681 gtcgttcggc gcgagcggaa tgttggccgg gccttgcccg ggcgtcggcg cccccggatc
     4741 ggcgagccag acttgccaag agcaggccgg actgtccgtc gggcgcgtga gctgcggctc
     4801 cagccagccg accgggccgc gggcggcctc ggcgacattc cccgcctcgg cggcgctgtg
     4861 cggcttgatc cgttgcagga gcgcttgatc ggcgggcgcc agatgcaggc cgatattggc
     4921 gatgaccgcg tccgcctcgg cgagactcgg cgcggcgatc gcccagccga cgcggctggc
     4981 gccggaatag gctttgacca aggcggcaaa accggcaatg tcggcgtcgc tggcgagccg
     5041 cgccggcgtc gcctcggcgc gccctgcggc aagcccgaga agcagcgcca tcagcacgga
     5101 ccaggcgacg aagggagcgc ggcggcgtct gtccgaacgg ctcgcaaagg ctttcatttg
     5161 tccggccatg gcttcgcctc tcactaagcc gggcactgtc cgggatcgtt gacgactttg
     5221 aactgatact ggatttcggc gccgaggcga tcttcgagga agctcgtcgt cgtggtcagc
     5281 acggcggcgc ccgacagccc cttgaggcgc ttgcgcaaat cgtcctgcaa ggcggtgatc
     5341 tcgaccgacg ggtcgcgcgt cgcgaccacg aacaggcgca cctcgtcctc gaccggctcg
     5401 accggaaccg ccatggtcag gtcgatcagg tcgtccgtcc ggctcgccgg cagcagcgag
     5461 cggaactggt tggcgtcgag aatgaccgga tcggcggcgc gcgacaccga ggcgatgaaa
     5521 atccgcggcg gccgcaaccg gctggcgaag ggcaggccat cgcgcgcgcc gacccgcagc
     5581 agcaaggtct cgccgccgat caccagcggc tggcgctgcg cgtcatagca ggggccgagc
     5641 aattggaact tgtcgctcgc cgcgtcgtag cgcgcccgct gcggcgtcgc ttcgggcgcc
     5701 ggcgcggcga gcgagcgcgt cgcgccctcc gcgatggtca ccgggcgcca ggcgagctcg
     5761 atgggcgcat tgcgccaggc gacccatccg ccgacgacaa gcgccagggc ggcgcaagcg
     5821 gcgatcgccc agagcgcgcg ggccggggtg acgatctcgc gcaacgcgaa cgggccgagc
     5881 gcctgctcca cgccttccag cgtgtcgacg aagacgaact cgatcttggc gccggcctcg
     5941 ccggcctccc gtttgagccg ctccagcacg ctcgcttcgg cgaggctgag cggcctgccg
     6001 tcgggcgtcg tggtcggcag gacgacggtc atcttcgccc cttccagcgc cttgcggtgc
     6061 tggaccacat agtcgccgac cagcagcagc ttgccgcgca gcccgtcgac cgggccgacg
     6121 ccgaccgtca gctcgccggg gctcgccggc aagcggattt cgccggtcgc aaagacgatg
     6181 ccggggtcgc gcccgaaagc ctgcaggatg gcggccaggg tcaggccaag ccccgccgag
     6241 cgggcgttct cgtcggagag gcggccgacc tgcagatcgg aaatgcgcag gttccaggga
     6301 ttgaccagcc cgttgcggcg gccgagccac aatttctcgc ggcgggcgtc gatgaagcgc
     6361 gtcaccgcct cgcgcaccgt ctggccgatt ccgtaaaggc cgaaatcgaa cagttcggaa
     6421 atttgcggca agagccgcgc gggatgcgcg tcgggcggga catccccggc tatcgcctcg
     6481 aagacttgcg cggcttcgcc attcgcgccg acaagaggca cgaagacgcg tgcgctcatg
     6541 gcgcttcgtc caggagttcg agctcgaagc tcgaaagatc ggcctcctcg atcgccgcgc
     6601 ccgccaggct cagccgcatg cggccgtcgc ggtcgaacgc gccgtcaagg gcgcgcccgc
     6661 cggcgaaggt cgcgcgggcg gagcgactgg cgtaaagcga caggccggcg acgccctggg
     6721 cctgcaattc gaggaccggc tcggccgccg gctcgctgac cagcttggcg aggaccaggc
     6781 cgtccgcgct gagcaggccg gaagcgggaa agcgccactc gcccgggcct ttcgccgcca
     6841 gggccatgaa ggccaggcgc ggccggctgg ccgcgcggcc gccggacaat gcgtattccg
     6901 ccagcgcggt ctgcgtcgcg acgcgcaaag tcctcagaaa tctcgccgcc tgggcgtcgg
     6961 tgaattcgcc cgccatggtg gtcccctatg accctcgcaa aatggcgctg aaaaggcgcg
     7021 ccggcctcga cgcatggtta cggtctggcg gcgccggccg cctggatgaa tttcgcggtt
     7081 ctcgccgaga gatcattgat ataggcctcg agatcgttgt cgaaatcggc cttcaggcgc
     7141 cggcggagat agaggttgga gcccatcgcc tcggcgagcg agcggccggc catgatcgcg
     7201 acgagcaggc gcttctcctc ggtctcgatc gcgccggcct tgaccgccat ctccagaaag
     7261 ggcgcggcct cctccgcatc catcagaggc tcgtccgccg ccgcctcgac ctcttcgctt
     7321 aaggtcgcct cgaccgcctc gaaagaggtc aggaaggtct cgtcctggcc ggcgaggcgc
     7381 gtcaggatcg ccgagccgag cggcgtcgcg gcgaaggact tgacgtcctt gaagcctgga
     7441 acgggcgcct gcgcgaaagc ggcctccagc tccgccgggc tggggcggcg gccgaacacg
     7501 cctttcatct cgatcttgag cgccttcaga aaggcgttgg cccctttcag cgtgttgacc
     7561 ccgacggcct cgtcgccgac cagccattcc ttcgggcaga ggccgagccg caacacctcg
     7621 gcatagggtc ggttcgacgc atcgaggcat ttcaggatgc gattggagag ctcgaacagc
     7681 tccttcttct cggcgaagcg ccgcagcatg gtcgggccgc cgaccagggc cttccagatc
     7741 gagaggggca gttttggcct tggcgcgacc gggccgaccg cccggctcgc gaccagcgcg
     7801 gcgagattgt cggccgagga cgggccctcg ccgcgcgagc cgatcggccg gtcgagcaca
     7861 ttgtcgaaat agaaatccgc cgcgtcctcg accgtgttcg gcggcggata ggcccagacg
     7921 ccgaccagaa cctcggaata tttgcggttg gcgacgaggc tgcgatggac cttctcgtag
     7981 agcgagaaag gcttgagcga aacggtctcg tccgcgtcgc tcaaggaatc gttgccgtta
     8041 accagattgg cgagatggcc ggcgaaatag gcgaccacgt cccggccgcc ttttgcagag
     8101 gtggacgcgc tcatgccttt cctttccggt cccggccgga ctccaaggaa acggccgctg
     8161 gacactggtt tagcaattcg gccgcccgcc ttaaagcgag aagcgcgttc attcccgcgc
     8221 aagcgggaat ccagtacggt ctatcccgca acactgaggc gtctggattc ccgctttccg
     8281 cgggaatgac atcgcttgat tgtcaggagc cggcgtccgc aagcgaggtc gcatagaccg
     8341 ccgattcctc gcgcagcacg ggtatggagc cgatgatcgg ataggcgagg cgagaagctt
     8401 cgctccaata gcaatcgccg catttttgca tcggcaggcg ggtcgccgga caggcccatt
     8461 cgtcgtcgtt gcgcgacgcc ccgccgggcg gcgtgacgat ataggcgtaa gtcggattga
     8521 gcggattgga gatggcggcg atcggccgct tttcctcgag gcgcccgccc tgcgcggcga
     8581 tcgcctcggg caggccgcgt atatagccga gcgcgtccat tcgcgcccgg ccggcggcgg
     8641 aattgtcttc ataagagggc tcgaacagca cgacgcgctt gcgcgcgacg cggaacaact
     8701 cggcgagcgc cgcctgctcg cgcccggcat tgggctccaa tgcatgcgag gtccagacca
     8761 cgtcgattga atgatcgcgc aagggcaagc gcaggaggtc gccgacgaaa ctcgacattg
     8821 ccgaggcgag atcggcccgc atctcggcgg cggcgaaggc gcggcccttt gcgatgcggc
     8881 tccaggaaat atcggccgcg tagtgacggg agattccggc gtagcaggca ttggcgacgc
     8941 cgaccagcgt ggtcatttcg ccggttccgg cttcgagcac gcgatcgccg gcggcgatat
     9001 ggtcgcgcag gacgccggcg agctcggacg tgtaagcgcg ccacaattcg cgattggcgc
     9061 ggacatgatc gatatagccg cccgcctgca gatcataggc gatctcgatg atctgcggcg
     9121 tgttggccgt ctcgccgagc gtctcgcgca aggtctgcat gacattgcga ccttcgcgat
     9181 aggcggcgat ggcgggacga agatcgcaat atttcatctg tgactcgttt gttcaaaaac
     9241 ccgcctgtca ttcccgcgaa agcaggaatc cagcgcggcc agcctgcaag cgccgaggat
     9301 actggattgc cgcttgcgcg ggaatgacgc gtcaccttga tggtacgccg ctcaatccgc
     9361 aactcaacag ccgaacggag ccggaagggg cgtcaaaggc gaaatgcaac ggaataatcc
     9421 aagtctcggc gaaatgggtt acggcggcgg gatcgatcgt catccgcact tcgttccgcc
     9481 ccgccgcaaa agcggcgccg tccagcgcgc caggcgcatc ggcttcgaag caaatgtcga
     9541 gcgcattgga ttccctcggc
//
LOCUS       DATGJW010000207         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_868400, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000207 DATGJW010000000
VERSION     DATGJW010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_868400"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VKU03_06515"
     CDS             <1..199
                     /locus_tag="VKU03_06515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70951.1"
                     /translation="IVLAAAALAVGLGVAPAFAEDAAKPAPAPMVHHHHHHHHHHHMM
                     RHHHHHHHHHHMMKKEEPKKS"
     gene            complement(386..1339)
                     /locus_tag="VKU03_06520"
     CDS             complement(386..1339)
                     /locus_tag="VKU03_06520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HLJ70952.1"
                     /translation="MRDPYEVLGLAKTASAGEIKSAYRRLAKKFHPDQSKEPRAKDRF
                     AEINLAYEILGDEKKRAEFDRGEIDAEGKPRAPGFEDFMRSRRGRARQGAGPDFSHYS
                     FDFGDGERVGGGAAGVDPDFLSELFNLRGGGASRSRAPLRGEDVVALATVPLALAATG
                     GSARVTLPTGKTLDVEIPVGVEEGKQIRLRGQGQPGARGGPAGDAMVTVRYAPHPLFK
                     VEGRDLRFDLPITLYEAVLGAKVRAPTLSGEVEITVPRGASSGSVLRLRGKGLPAAGE
                     SAQGDLYATLRIVLPGQTDPELEALMRRWRDEKPYNPRVGM"
     gene            complement(1452..1856)
                     /locus_tag="VKU03_06525"
     CDS             complement(1452..1856)
                     /locus_tag="VKU03_06525"
                     /inference="COORDINATES: protein motif:HMM:NF028308.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RT0821/Lpp0805 family surface protein"
                     /protein_id="HLJ70953.1"
                     /translation="MTASAGLAAALAGCALSFPLPSLMSEDATGSIKARPGPFARDLD
                     EADWRIAEPRLAEALKSGIEDPPKQWDNPDSGHRGAFQPVAGAFKRDGQTCRAFLARI
                     NAGEGSKTMQGIGCLMAGDTVFVDDAQPWKAL"
     gene            1989..>2340
                     /locus_tag="VKU03_06530"
     CDS             1989..>2340
                     /locus_tag="VKU03_06530"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase"
                     /protein_id="HLJ70954.1"
                     /translation="MKELTSGDFTEAVDPFALFRQWFEEARASEPNDPEAMALATVDA
                     DGLPNARMVLMKAFDETGLVFYTNEESAKGRELAAAPKAAALFHWKSLRRQIRLRGAV
                     AAVSEAESDAYFRSR"
BASE COUNT          392 a          813 c          744 g          391 t
ORIGIN      
        1 gatcgttctc gccgcggctg cgctcgcggt cggcttgggc gtcgcgccgg ctttcgccga
       61 agacgccgcg aagcccgcgc cggcgccgat ggttcatcac caccatcatc atcaccacca
      121 tcaccatatg atgcgccatc atcaccacca ccaccatcat catcacatga tgaagaagga
      181 agagccgaag aagagctgag cgcggcgcca gccgacgagc ttttttgggg acgaaatccc
      241 ggccgcgacg agcggaccgg gatttttctt tttcgcgcga tcgtcctatg cgggaatcca
      301 gtacgaccta tcgcgcggcg gctctccgga ttcccgcttt ccgggaaacg acaacgttcc
      361 gtcttcggta cgtacggcgg cccgctcaca tcccgacgcg tggattgtac ggcttttcgt
      421 cgcgccagcg gcgcatcagc gcttcgagct cgggatcggt ttggcccggc agcacaatgc
      481 gcaaggtcgc atagagatcg ccctgcgcgc tctcgccggc ggcgggcagg cccttgccgc
      541 gcaggcgcag cacgctgccg ctgctcgcgc cgcgcggcac cgtgatctcg acctcgccgc
      601 tcagggtcgg cgcgcgcact ttggcgccga gaacggcctc atagagcgtg atcggcaagt
      661 cgaaacgcag atcgcgtccc tcgaccttga acaaaggatg cggcgcgtag cggacggtca
      721 ccatggcgtc gccggcggga ccgccgcgcg cgccgggctg tccctggccg cgcaggcgga
      781 tctgcttgcc ctcttcgacg ccgacgggaa tctcgacatc gagcgtcttg ccggtcggca
      841 aggtcacccg cgccgagccg ccggtcgcgg cgagcgccag cggcacggtg gcgagcgcga
      901 cgacgtcctc gccgcgcagc ggcgcgcgcg agcggctggc gccgccgcca cgcaaattga
      961 aaagctccga gaggaaatcg ggatcgacgc cggccgcgcc gccgccgacg cgctcgccgt
     1021 cgccgaaatc gaagctgtaa tgggagaaat cgggtccggc gccttgccgc gcccgcccgc
     1081 ggcggctgcg catgaaatct tcgaagcccg gcgcgcgcgg cttgccctcc gcgtcgattt
     1141 cgccgcggtc gaactcggcc cgcttcttct cgtcgccgag aatttcataa gcgaggttga
     1201 tttccgcgaa acgatccttg gcgcgcggct ccttgctctg gtcgggatgg aatttcttag
     1261 caagccgccg ataggccgac ttgatctccc ccgcgctcgc cgtcttggcg agcccgagga
     1321 cctcataagg atcgcgcatg atgagcccgt cttcggcgtc cgcccaaggg cgcgggcgcc
     1381 ctgtctcgat tcctcaattt gggcagccgg cgcgtccggc gcaagaggag ccttgcctta
     1441 agcttggctc gctacagcgc tttccacggc tgcgcgtcgt cgacgaacac cgtatcgccg
     1501 gccatcaagc agccgattcc ctgcatggtc ttgctcccct cgccggcgtt gatgcgagcg
     1561 aggaaggcgc ggcaggtctg tccgtcgcgc ttgaaagcgc cggcgaccgg ctggaaagcg
     1621 ccgcgatggc ccgagtccgg attgtcccat tgtttcggcg ggtcctcgat ccccgatttc
     1681 agagcctccg cgaggcgcgg ctcggcgatc cgccaatccg cctcgtcgag atcgcgggcg
     1741 aagggaccag gacgcgcctt gatcgagccg gtcgcatcct cgctcatcag ggaaggcagc
     1801 gggaagctca gcgcgcagcc cgccaaagca gccgccaagc cggccgacgc cgtcaatccc
     1861 aatagattcc tcaatttccc gccgaaactt ttgccgcgct cgtctggaat catgaatcgg
     1921 atcatattgg gcaagatcgt ctcggccggc cggctttaac gcgcgacaac cattcgggag
     1981 atttcacctt gaaggagtta acgtctggtg acttcaccga ggccgtcgac ccgttcgcgc
     2041 tgttcaggca atggttcgag gaggcccgcg cgagcgagcc gaacgatccc gaagccatgg
     2101 cgctggcgac ggtcgacgcg gacggcctgc ccaacgcccg catggtgctg atgaaggctt
     2161 tcgacgaaac cggcctcgtc ttctacacca acgaggaaag cgccaagggc cgcgaacttg
     2221 cagccgcgcc caaagccgcg gcgctcttcc attggaaatc gctgcgccgg caaatccgcc
     2281 tccggggagc cgtcgccgcc gtgagcgagg ccgaatcaga cgcctatttc cgcagccggc
//
LOCUS       DATGJW010000208         3541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_869000, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000208 DATGJW010000000
VERSION     DATGJW010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3541
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869000"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(181..1077)
                     /locus_tag="VKU03_06535"
     CDS             complement(181..1077)
                     /locus_tag="VKU03_06535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ70955.1"
                     /translation="MRVFVTGATGFIGSPVVKELIACGHRVLGLCRSDEKAAALAAAG
                     GEVYRGSIDDLDGLKAAAAQADGVIHLAFKHDFSNFFANCENDRRVIKALGSALAGSG
                     RPLIVTSGTGIANTVPGQPAKEDNATVGSAVHPRAASEEAAAAVAATGVNVTVVRLPQ
                     VHDTIKQGLITPAIEIYRAKGACAYIGDGLNRWPAAHVLDVARLYRLAIEKAEPNAKY
                     HAVAEQGVPVRDIAEALGRRLKLPVKSVAPEEAPAFFGWLAVFAGYDMPTTSEQTRRK
                     LGWTPIGPGLIADLERLQGFDR"
     gene            1170..2018
                     /locus_tag="VKU03_06540"
     CDS             1170..2018
                     /locus_tag="VKU03_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050597.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ70956.1"
                     /translation="MSERLAAENPLGAYLRDRRAKLDPGALGFSSERRRTLGLRREEV
                     AQRANISPTWYTWLEQGRGGAPSANVLDRIARALMLTDIEREHLFLLGLGRPPEARYR
                     KSDEITPRLQRVLDALNPSPAVIRTALWDVVAWNRAATAMLMDYGALPPEERNILRFI
                     FLDPRARAAQYDWESVARFALGAFRVDAARAGAAAEVEPLVEELCRLSPEFKAMWRDN
                     DVSGPHGETVKHIRHAIVGPLAFEYSAFSVDGRTDLAMVVYNPATPADAERIKALMRE
                     QEAAPR"
     gene            complement(2046..2453)
                     /locus_tag="VKU03_06545"
     CDS             complement(2046..2453)
                     /locus_tag="VKU03_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ70957.1"
                     /translation="MGLQSAGLQSQGTVLVVEDEFLILMELSKCLRDAGYGVIEASNV
                     AEARTALHSWSRIDVAVLDIRLPEPADGVRLVKWIRANRPEVEAIIATGYDADEELRS
                     LAPVVAKPYRSDELLGVIRKIMAKRRQAAEEAR"
     gene            complement(2534..2731)
                     /locus_tag="VKU03_06550"
     CDS             complement(2534..2731)
                     /locus_tag="VKU03_06550"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HLJ70958.1"
                     /translation="MPQDEEQIRKIAHKIWEDVGQPIDQAKLHWEMAEKELEEAEAAK
                     KTADPPKESDTLPVLKSPPLV"
     gene            2886..3257
                     /locus_tag="VKU03_06555"
     CDS             2886..3257
                     /locus_tag="VKU03_06555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70959.1"
                     /translation="MRTIDEIIDDAQRAAAESLRAAFASGGAEIASALRARMEAAVEP
                     ARDAPADATSAAAGGEAPRLETPELIAKPALRAVLLSAAAMLTLALWHARAARILPAC
                     ALLIGLGVVLRDLSGEDGPAD"
     gene            complement(3278..>3541)
                     /locus_tag="VKU03_06560"
     CDS             complement(3278..>3541)
                     /locus_tag="VKU03_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA ligase"
                     /protein_id="HLJ70960.1"
                     /translation="RPLLTRRLEKLRQPPGFTGKAPGGPSRWSTERSGEWRPVRPELV
                     VEVRFDHATDGRFRHGTKLLRWRPDKAPRQCTFEQIAPHPRRA"
BASE COUNT          565 a         1190 c         1155 g          631 t
ORIGIN      
        1 cgaaggggcg ctgatggcaa gcgtctatcg tcctgcacac tccgcaacgg cgcgacgcgc
       61 cgtcattttg agcggagcga agcccattca tcgggtacgt ccttcgaatg gatcgcttca
      121 tcgctgccct cctcgcaatg acaagccgca tcttcgcgac cggcggcatg gcgccgcggc
      181 ttagcgatcg aagccctgca gccgttcgag atcggcgatc aggccaggcc cgatcggcgt
      241 ccatcccagc tttctccggg tttgctcact tgtggtcggc atgtcgtacc cggcaaatac
      301 agcgagccag ccgaaaaagg ctggcgcctc ttccggagcg acggatttga ccggcagttt
      361 cagtcgtcgg ccaagcgctt cggcaatgtc gcgcaccggc acgccttgtt cggcgacagc
      421 gtggtacttc gcgttgggct ccgccttttc gatcgccagc cgatagaggc gggcgacatc
      481 gagcacatgc gccgcgggcc agcgattgag cccgtcgccg atataggcgc acgcgccctt
      541 tgcacgataa atctcgatcg ccggggtgat gaggccctgc ttgatcgtgt cgtgaacctg
      601 cggcaggcgc accaccgtca cgttgacgcc agtcgcggcc acggcggcgg ccgcctcctc
      661 ggaggcggcg cggggatgca cggcggagcc gacggtcgca ttgtcttcct tggccggctg
      721 gccgggaacg gtgttggcga ttccggtccc ggacgtgacg atcagcggcc ggccggaccc
      781 ggcaagcgcc gaaccgagcg ccttgatcac gcgccggtca ttctcgcaat tggcgaagaa
      841 attcgagaag tcgtgcttga acgccaggtg gatgacgccg tccgcttgcg ccgccgccgc
      901 tttgaggccg tccaagtcat cgattgagcc gcgatagacc tcgccgccgg ccgccgcgag
      961 ggccgccgcc ttttcatccg atcgacaaag gccgagcacg cgatggccgc acgcgatcaa
     1021 ctctttgacg acgggcgaac cgatgaatcc ggtggcgcca gttacaaaca cacgcatgag
     1081 caaagtctcc agtttcgctg gagacaaatg tcggcgcatt cgctattctg gtaaagtcga
     1141 tagtttatca tagtaacacg gctaacagga tgagcgagcg gctggcggct gaaaacccgc
     1201 tcggggccta tctgcgggac cgacgcgcca agctcgatcc gggcgctctc ggtttttctt
     1261 cggagcgccg ccgcacgctc ggcctgcgcc gtgaggaagt ggcgcagcgc gccaatatca
     1321 gccccacttg gtacacgtgg ctcgaacagg ggcgcggcgg cgcgccctcg gccaatgtgc
     1381 tcgaccgcat cgcccgcgcg ctgatgctca ccgacatcga gcgcgaacat ctcttcctgc
     1441 tcggcctcgg ccgtccgccc gaggcgcgct accggaagag cgatgagatc acgccgcgtt
     1501 tgcagcgcgt gctcgacgcg ctgaatccga gcccggcggt gatcaggacc gcgctatggg
     1561 acgtcgtcgc ctggaaccgc gcggcgaccg cgatgctgat ggactacggc gctcttccgc
     1621 cggaggagcg caacatcctg cgcttcatct ttctcgaccc gcgcgcccgg gcggcgcaat
     1681 acgactggga aagcgtcgcg cgcttcgccc tgggcgcgtt ccgggtcgac gcggcccgcg
     1741 ccggcgcggc ggctgaggtg gagcccctcg tcgaggaact ctgccggctc agccccgagt
     1801 tcaaggcgat gtggcgcgac aatgacgtca gcggcccaca tggcgagacc gtcaaacaca
     1861 tacgccacgc cattgtcggt ccgctcgcgt tcgaatattc ggctttttcc gtcgacggtc
     1921 ggacagacct cgcgatggtg gtctacaatc cggcgacgcc cgcggacgcc gaacggatca
     1981 aggcgctgat gagggagcaa gaggctgcgc cgcgctgaac gcctatggta cggcgctcgg
     2041 tatgctcaac gcgcttcttc ggcggcctgc cgacgcttcg ccatgatctt gcggatgacc
     2101 ccgagcaact cgtcggaacg atacggcttc gccacgaccg gcgcgagcga gcgcaactcc
     2161 tcgtcggcgt catagccggt ggcgatgatc gcttcgactt cgggtcgatt ggccctgatc
     2221 catttgacga ggcggacgcc gtcggccggt tccgggaggc ggatgtccag caccgcgacg
     2281 tcgatgcggg accatgaatg aagcgcggtg cgcgcttcgg cgacgttcga ggcttcgatg
     2341 accccgtagc ctgcgtcgcg cagacatttc gaaagctcca tcaggatgag aaactcgtcc
     2401 tcgacgacga gaaccgtgcc ttggctttgc aggcctgcgc tttgaagccc cattcgtcgc
     2461 ccttcccggc ttgacgcgag acgcgacgaa cgccggcgga ccgtcgattg ttccaggccg
     2521 acgcaatgag cggctaaacg aggggcggcg atttcagcac cggcagcgta tcggattctt
     2581 tcggcggatc ggcagtcttt ttggccgctt cggcttcctc cagctccttc tcggccattt
     2641 cccaatggag cttcgcctgg tcgatgggct ggccgacgtc ctcccatatt ttatgcgcga
     2701 ttttgcgtat ttgctcttca tcctgcggca tttccgctgc cctttctctg aggctcggcg
     2761 ggtcgggact gattctctcc aacgcgccat cggccgtccg gttccgcttc ggtcatctcg
     2821 ccctttgacg acttcttagc gcatcggaaa atatcgtaag atgccgccgc cagcgagtct
     2881 tcgccatgcg caccatcgat gaaatcatag acgacgccca gcgcgcggcc gccgaatcgc
     2941 ttcgcgccgc cttcgcgtcc ggcggcgcgg agatcgcgtc ggcgctcagg gcgagaatgg
     3001 aggcggccgt cgagccggcc cgcgatgcgc ccgccgacgc gacgtccgcg gccgcaggcg
     3061 gcgaagcgcc gcggctcgaa acgccggagc tgatcgcgaa gccggccctc agggccgttc
     3121 tcttgagcgc cgccgcgatg ctgaccctcg ccctctggca tgcgcgcgcg gcgcgaatcc
     3181 tgccggcttg cgcactgctg atcgggcttg gcgtcgtctt gcgggacttg agcggcgagg
     3241 acgggccagc cgattgaagg cgagagcgcg cccggcgtca cgcccgccgc gggtgcggcg
     3301 cgatctgctc gaatgtgcat tgccgcggcg ccttgtcggg acgccagcgc agaagtttcg
     3361 ttccgtgccg gaagcggccg tcggtcgcgt ggtcgaatcg aacctcgaca accagctccg
     3421 gccgcaccgg gcgccattcg ccgctgcgct ccgtgctcca gcggctcggc ccgcccggcg
     3481 ccttgccggt aaagccgggc ggctggcgga gcttttccaa gcgcctggtc agaagcggac
     3541 g
//
LOCUS       DATGJW010000209        19058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1387435, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000209 DATGJW010000000
VERSION     DATGJW010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 19058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 19058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..19058
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387435"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..258
                     /locus_tag="VKU03_06565"
     CDS             <1..258
                     /locus_tag="VKU03_06565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLJ70961.1"
                     /translation="RDRAYFGHGREEEDEIGFIDEKTRAIFGRSYAAEPDALIEQLAQ
                     DEAIAAADTLLLTVPNQLGVDYNAHVIEAILTHVAPALGWR"
     gene            368..910
                     /locus_tag="VKU03_06570"
     CDS             368..910
                     /locus_tag="VKU03_06570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLJ70962.1"
                     /translation="MRHESKAASAGHKPAIDPHLFRRTMGAFATGVAVITVDSGETIH
                     AMTANAFMSGSFEPPLCVISVAKRAHTHQLIRQAGRFGVNVLGADQQDLALYFAGKKS
                     LDVEASFQRVGDTPLLRDCAARIAASVANAYDCGDHTLFVGAIHHMDSNDRPPLLYHR
                     SAFGALAPKARERVPVPEFW"
     gene            complement(927..1994)
                     /locus_tag="VKU03_06575"
     CDS             complement(927..1994)
                     /locus_tag="VKU03_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ70963.1"
                     /translation="MSLPSLRQEWPKPSSPRPIVIIGAGGIVKDAHLPAYRKAGFPVA
                     GLFDLDRGKAEALAAQWGVPRVFASLGEAAKQSGVVFDIAAPPVAHRDILAALPEGAA
                     VLIQKPMGMDLDEATATLRLCRERRFKAAVNFQLRFSPMMLALRDALAKGLLGRLIDI
                     EVHLNLVTPWGLFPHLKVNPRVEIVSHSIHYLDLIQSLVGLPKGALARSYGHPSSDLT
                     DTRTTAILDYGGDLRVALSINHHHDFGRRFQDATFRFEGDQGAAMVKLGLLLNYPTGE
                     PDELWIAKRGEEAEQVPLAGGWFPDAFIGVMANVQRYAAGEDAIMETSVESAWRTMAL
                     VEACYRSGAIAGAPIAPPPNA"
     gene            complement(1991..3178)
                     /locus_tag="VKU03_06580"
     CDS             complement(1991..3178)
                     /locus_tag="VKU03_06580"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLJ70964.1"
                     /translation="MTETIRIAVRKFPPFETAIVRQFQDFVRVAGVDARIEIEVFDLN
                     PLHERLFERQALAKGEFDIAFLSTDWIAEAHQAGALADLAPHLRRAPIPDFPDAWSPS
                     LLNLQQFAGGFWGMPYHDGPQCLIYRKDLFDAAGLSPPKTWDEFVAIARRLHKPEAGR
                     YGTVLALFPDGHNSFYDFCVHVWTRGGEPFAPDGRPDLVTKEAIAALDFIRALAADAT
                     AMAPDPRALDSVKSGLMFCEGKIALMTNWFGFAALGDSIEGPVKGKIDIAPLPSAGGR
                     SVSLNVFWMLTLASGSRNPDLAWTFMRHAASPAMDLLTTREGAIGVRRSTWSDREVNA
                     RIPYYGKLDRLHEFARELPRHPRLADMAHIIDEMLSRALASPTPSRDLLADAEAKIKG
                     VLQ"
     gene            complement(3175..3978)
                     /locus_tag="VKU03_06585"
     CDS             complement(3175..3978)
                     /locus_tag="VKU03_06585"
                     /inference="COORDINATES: protein motif:HMM:NF013757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HLJ70965.1"
                     /translation="MAEKVLSIEERRSKYAVPALEKALDIIEYLSDQAVPMTQAQLGR
                     ALGRQPGELFRMLTALETRGYLRRDAVTGGYALTLKLFELSRTHSPYEALLRAAVPIM
                     RQLADEVRETCHLSAIHRDKILVLAQEESPKPIRLSIEVGSLHSPLSTASGRILLGAM
                     EKDERDAFLSSATNFFSRPEEERTAFLNRVHAVQSRGYEIAEGERFVGGLDLGVLVGG
                     TASAVRAALVIATLKYAEGPDMEAMLPALRQAAAAIAEQAGLKTDKGAP"
     gene            complement(4050..5180)
                     /locus_tag="VKU03_06590"
     CDS             complement(4050..5180)
                     /locus_tag="VKU03_06590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006339951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70966.1"
                     /translation="MNTKDWRRHFGLREAGVYYALILLMAVIGLITASSGGPNYFSAA
                     NLTNILYQASLVGMMGVAMTVILITGNFDLSVASVAALSGAVLISLAGAVGFWPAVCV
                     AMLVAVAVGLLNGAIVQLLGINAFIVTLGTLTAIRGLVLIVTNSRSLLVEDPAVKAQM
                     VAFEGGRAPIQAALFAIGAVLIAGAAYAMVRRKGGAPIGVAIGGAVLILLGVLGGAGL
                     TVAKPVIYTAIYTLIVWFVLGFTTIGRRVYAVGGNAEAARLSGVKVGLYKMAAFVLCS
                     ATAGFAGILFACRLGAINPTALQGAELTVIAAAILGGTSLFGGAGSVVKTLAGALLLF
                     ALANGFNILNLGANYQGLIEGTVVVAAAAIYTVGGARRRRAA"
     gene            complement(5189..6703)
                     /locus_tag="VKU03_06595"
     CDS             complement(5189..6703)
                     /locus_tag="VKU03_06595"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ70967.1"
                     /translation="MLQLRKVEKIFPNGTVALRGVDFDAYAGRVHGLLGANGAGKSTL
                     INILSGAAAASAGTIAWRGTPVAFKTPAEANAAGIATIHQHIPLVPTLSVLENVFLGR
                     GGVWRRNPDDRARFEALCRDVGYAIDPDALVGDLPIGKRQMVAIMQALGGGADLIVMD
                     EPTASLANEEREIVYETVRRLAGQGKGIVFVSHFLDEVIALTDEVTVLRDGREAMRAK
                     TKDVDERAIAEAIVGKAVATLDHLREGREPVAGPELLVVRNLASGDRLRRIDLVVRKG
                     EVVGLAGLLGSGRSELLHAIFGADRGATGEVLLDGKPLPRATSGAVSAGVALVPEDRK
                     AQGLIPAFEIWRNVTLPHLDDTAVGRLLTRRDVEWARGESAIERLRIKAPSPETLVGE
                     LSGGNAQKVTVAKWLFGEVKLLLLDEPTAGIDVGAKADILALTRRLAASGVAIIIVSS
                     EFEELLAVCDRILVMRDGAIVAERAASATSDHELVLLAGGARGAQAEPTAVALH"
     gene            complement(6909..7880)
                     /locus_tag="VKU03_06600"
     CDS             complement(6909..7880)
                     /locus_tag="VKU03_06600"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ70968.1"
                     /translation="MTMTMKRSISAWTLAAALSAAAAAPAFAADTYGFSNVGLSFPFA
                     AAIAKGFQDAAKQAGVTAVVLDAKNDVQKQANDIDDLIAEKAKGIVIMPLDSTVAQGW
                     VDKVAAAGIPVASVGSQIGDPNKRAIKDVYPKLVAVVTQDEIAAGNRAGELALKLLPK
                     DRVAKIAIIEGQAGFSEVKQRSQGFLEALDKAHAKYQVVASQPGDWTAEKGEAACQNI
                     LSAHPDVDLFFNQADDMVVGCARAVKAAGSKAKLIGFGGSKLAIAAIKDGSVDGTVCY
                     KPEELGHLAFQAVYEAATGKNVRHHEFITYDTPGVTKANIDQCVGQW"
     gene            complement(7950..8405)
                     /locus_tag="VKU03_06605"
     CDS             complement(7950..8405)
                     /locus_tag="VKU03_06605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC/PaaZ C-terminal domain-containing protein"
                     /protein_id="HLJ70969.1"
                     /translation="MIAEQYFEDYVIGFSRRSFGRTITEADIVLHAGQSGDFFPHHMD
                     AEWCATQDFKQRMAHGTLVFSVGIGMTAGAINPRAMSYGYDRLRFLKPVFIGDTITVS
                     ATIKEKRDHPKRNGFGIVAEAIEIVNQRNEPVLVCEHLYLVERRNPVAR"
     gene            complement(8395..9627)
                     /locus_tag="VKU03_06610"
     CDS             complement(8395..9627)
                     /locus_tag="VKU03_06610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLJ70970.1"
                     /translation="MPPRESETAPRAPANEAENSGLPLEGIVVLDFAQFLAAPSCALR
                     LADLGADVIKIERPQGGDLCRQLTIADQVFDGDSALFHTINRNKRSLTADLKKPADLA
                     LVKKLIARADVMIHNFRPGVMERLGLGYDEVSALNPRIVYGTVTGYGVGGPWRDKPGQ
                     DLLAQSLSGLAWLSGDAEQGPVPVGVSVADIFTGAHLVQGVLAALIRRGATGKGGRVD
                     VNLMASALDLQFEPFTAYLNSKAGQPRRSRVSNANVHAAAPYGVYATADGYMALAMTP
                     IGKLADLLSCEALRPYADQATWYRDRDAIKQVIADLLRTRPTAAWLALLEPADIWCAE
                     VFDWPRLETHPGFAALDAVQEVASSGGETMRTTRCPIQVDGRTLKSKRGAPRLGEHTQ
                     EVLAQFGLAGAAGASDDR"
     gene            complement(9847..10614)
                     /locus_tag="VKU03_06615"
     CDS             complement(9847..10614)
                     /locus_tag="VKU03_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HLJ70971.1"
                     /translation="MNALIDRYFKTLIDRLEAVRTSQEAAIDAAASASADSIAAGKLV
                     FAFGTGHGALPALEMFPRTGTIVGFRPIVESTMISFHRVWGDMGARQYRFIHAVEGYG
                     KAILSAHRLDPQDAMILFSHSGVNAVILDIALASKAKGLKTIAVTSLPHSQNTASRHS
                     SGKRLFEVADIVIDTHAPLADASLAIDGLEHPVGASSTSVTIAIAHAIVSATAEKLVA
                     RGVKPMVMVNPNTAGRDQANRVNDANYEALWRRLADR"
     gene            complement(10630..11376)
                     /locus_tag="VKU03_06620"
     CDS             complement(10630..11376)
                     /locus_tag="VKU03_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uridine phosphorylase"
                     /protein_id="HLJ70972.1"
                     /translation="MTRSAWYIGCHADEVGDSAILVGDPARIARLAAALDAPRMLEER
                     RGLRAVTGERQGRRLTAVAFGMGAPIATIVLHELRDLGVHTFLRIGTAQVMPPAVLGD
                     FVLADAALRAEGASLTYAPAGYPAAADFDLNAALRARLRQSGRGWRAGVFATYDGFYS
                     QMFALSDGQRRVIDDLRHDLVRYRILATDMETSALLAVGRLLGARASSLCMGTVNGLT
                     QETLPQAEQAICEREMFEIALDVLAALPKP"
     gene            complement(11373..12257)
                     /locus_tag="VKU03_06625"
     CDS             complement(11373..12257)
                     /locus_tag="VKU03_06625"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HLJ70973.1"
                     /translation="MTRIAISGYASLDYVVQLAGAPQPGRTVLVTHRAADAWPRPGGS
                     PTYIGAAIRRAGSADIALITWIGADLAGSDYIRHIAALGLSSDGVARSLPGPTPVCIL
                     AYDPAGRCYCFYEAAASAGAYNAAQLAMIEAADWVCLTVQPAAASRAALERISERQYL
                     IWAVKGDPNAFPDDLRRDLAARADLVIHSHGERGFVAPFLAAAPGRADRLVVETHGAA
                     GATLTMSGRTIRAPIEAEKRVAARDPTGAGDTFLGGLLAALMTKPNDPLAAVRAGETA
                     ARDMLTSRNERAAYGGEG"
     gene            complement(12254..12817)
                     /locus_tag="VKU03_06630"
     CDS             complement(12254..12817)
                     /locus_tag="VKU03_06630"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family cysteine hydrolase"
                     /protein_id="HLJ70974.1"
                     /translation="MDSSGETALILIDVINSFFRPGMPNHYPEAAATLDPLRRLLAAA
                     RASDRIVVHAVERHYPAFDDFEWRKLPKHHFIGDEDSAFVEGFGPAGPREIVSPKRRY
                     SAFFATDLALFLNEQRVRRLIVGGVKTNVCIRATIQDAFAHGFEVLLPREATNSNRPH
                     LAAASLEDIDRYFGKVVSLDEALKALA"
     gene            complement(12821..13720)
                     /locus_tag="VKU03_06635"
     CDS             complement(12821..13720)
                     /locus_tag="VKU03_06635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70975.1"
                     /translation="MIDNVLAAAALLTTPILLAAIGGLINRLGGIVNLGLEAMMLTGA
                     YVALAASSATQSWPLALAAALVAGGVVGYLFSLAVTRLQANEIVAGVGLNVAAAGLIG
                     FVLKDYYHSSGTIRLPDVVMLPRIVIPGVAQIPIVGAILSGKDPLTWLSWALVPITAF
                     VLSRTRAGLRLRAAGAAPEAARALGLDPLAIRDASTAVAGALAGLAGAQLSIGVVGVF
                     SVGISGGRAFIALAAFYFGRNRPGLTAAGAALFGLFDAIQIYLQGRGVAPELMQMLPY
                     IVVLVVLTAIGLGERRRRLWQGA"
     gene            complement(13717..14775)
                     /locus_tag="VKU03_06640"
     CDS             complement(13717..14775)
                     /locus_tag="VKU03_06640"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ70976.1"
                     /translation="MTAATTGVVRTSRLDWAALAAALAPFAVALIVGAILLAATGRNP
                     LAVYGLMLREGFGGESRVAATLTAATPILFTAIATAISFRAGIFNVGVEGVFSLGGLA
                     GAVAGFTLTALPSPLLIVAELAVGALVGGLWLAAPALLRARLEVDEVVSTLMLNFVAV
                     NLTLFLVNGPFLAPGSANSATPMIAAAGWLPRIAPPSTLSLGFIIALALLALYAVWDR
                     LTPLGFEARLNGLNPRFSRAIGIDAPRLILKVMIISGAIGGLAGSAHALGVTHRFVVG
                     FSPGYGWTGLAVALMGRNSAIGMLIGAIVFGALASAGTTVQLFSDIPLEIVDVLQGVV
                     TIFAVVRFGRLLRGRSAA"
     gene            complement(14772..16295)
                     /locus_tag="VKU03_06645"
     CDS             complement(14772..16295)
                     /locus_tag="VKU03_06645"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ70977.1"
                     /translation="MIKPAVRIGARGVTRRFGGVIANDAIDFAAAPGEIRGLVGGNGA
                     GKTTLMRILQGLEQPDEGAVILNDEPVRLADPADAFARGVGMVHQEFMLVEDLTLLEN
                     LILGREPVGPGGVIDRERALAEARRLAASAGVDLDWRLRVSEAPVHVRQILEILRLLY
                     RGADVLILDEPTAVLAPQQVRDLLNLLRRLRDEGRTILFISHKLDEVLTVCDAITVLR
                     AGKVVASTIPAEANIGRLAELMVGETIEFPRRLAQTRSSEKSALSLRGVRALDSRGVE
                     RLTGIDLDIGPGEIVGLAGVSGSGQDEIVAVITGLRRAIAGRVAIAGRDVTALSVAAR
                     RSAGLGYISADRAHEGLCLAETIAANVIAGRHRAKPFARRGLLWPKTIRTHANKALAR
                     FAVVYRDCQEAARTLSGGNQQRVVIARELDRNPRVLVAAQPTRGVDIAGAAFIHRQIL
                     AFAAAGGAVLLVSEEIEELLTLADRILVLYRGAVIGEVAGADRSVGRIGRMMLGEAA"
     gene            complement(16317..17375)
                     /locus_tag="VKU03_06650"
     CDS             complement(16317..17375)
                     /locus_tag="VKU03_06650"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLJ70978.1"
                     /translation="MGKSYLKKSCLALSVSALALVLSFAAPVAQAAEKAPVALIIAQG
                     GLGDQSYNDLADKGFKKALAETKLEGKEVESKDVVAQGENMLRRAADGGFGLIVDLEY
                     SHGDAIKTVAKDYPKVDFSILNLVEPGANIASVLFKEQEGSYLAGALAAMVASDPKIK
                     GIGPKPIIGVIGGTKSAGIDKFIAGYIQGAHDIDPKVEVKVAYSNNFGDPAIGHQMAK
                     AMFDEGASIVYQVAGGTGIGVIQAAKETGHYAIGVDTDQDALAKGHVLTSMIKRTDLA
                     VETVIKDYAADKFPGGQTVTLGLKEDGVGLSPMTYTKDLIPAADLAKIDDLKKKIIAG
                     EIKVWDVTTQGYPSWWKQ"
     gene            complement(17278..18144)
                     /locus_tag="VKU03_06655"
     CDS             complement(17278..18144)
                     /locus_tag="VKU03_06655"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ70979.1"
                     /translation="MHILKVDDGRPLYRRLSDELRAEIAALKPGDRIASEPELAQRLN
                     VSRFTVAKAIEGLVDEGLLVRRQGKGTFVAAQPLKRLPGQLRSFTDSVVAAGRRPRSE
                     LIGFGPVAWTPEAPYPADQGLIALDRLRFADGAPVAIHHSVLSQSVSDELGLNASLVG
                     RPDFSLYREMERAGLALDHCVEVLTARLPKPEERDLLRLGASDVVMVIQRQTYGVDGR
                     LLDHVAAIHDCRHYSYQALLMRHPHRGIPPALAMKESGDGKVVPEEVLPGAQRFGPRF
                     GPQLRGARRSGG"
     gene            complement(18382..>19058)
                     /locus_tag="VKU03_06660"
     CDS             complement(18382..>19058)
                     /locus_tag="VKU03_06660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ70980.1"
                     /translation="GKSRGVGVIALDHPTVREAIRALSAAGVKVLTLASDISHVPRVA
                     YVGIDNRAAGRVAGYILGRFLGNSERKKVALFAGSLSYRGHEEREMGFRHILAEEFAN
                     LEVVELREVKDDFEKAFVEADNLLKRHPDIAGVYNIGAGTEGIARALRDRSRAKEVVF
                     VGHELTDANKALLLDGTLDAVIDQNPRVEAREALNILTHSVRGAPYEYHAPRLQIICK
                     ENLPEV"
BASE COUNT         3328 a         6300 c         6378 g         3052 t
ORIGIN      
        1 cgcgaccgcg cctatttcgg ccatggccgc gaggaagagg acgagatcgg cttcatcgac
       61 gagaagaccc gggcgatttt cggccgcagc tacgccgccg agccggacgc cctcatcgag
      121 caattggcgc aggacgaggc catcgcggcc gccgatacgc tcctcctgac cgtgccgaac
      181 caactcggcg tcgactacaa cgcccatgtc atcgaggcga tcctgaccca tgtcgccccg
      241 gcgcttggct ggcggtgacg gcggttcggc cgcggcgtga tgagcgcccc gaattgacgc
      301 tccgtatgcg tggtttaagc gcgccgccga cctcaccgtt cttgccgagt tcaggtatca
      361 tcgaaccatg cgccacgaat ccaaagctgc ttccgccggc cataagcccg ccatcgaccc
      421 gcatcttttc cggcgaacga tgggcgcttt cgcaaccggc gtcgcggtta tcaccgtcga
      481 ttccggcgag accatccatg cgatgaccgc gaacgccttc atgtccggct cgttcgagcc
      541 gccgctttgc gtgatttcgg tggccaagcg cgcccacacc catcagttga tccgacaagc
      601 cgggcgcttc ggcgtcaacg ttctcggcgc cgaccagcag gacctcgctc tctatttcgc
      661 cggcaagaaa tcgctcgacg tcgaagcgag ctttcagcgc gtcggcgaca cgccccttct
      721 gcgcgattgc gcggcgcgga tcgcggcgag cgtcgccaac gcctatgatt gcggcgacca
      781 taccttgttc gtcggcgcca tccatcacat ggattcgaac gaccgcccgc ctttgctcta
      841 tcaccgcagc gccttcggcg cgctcgcgcc aaaggcgcgc gagcgcgttc ccgtcccgga
      901 attttggtga gcttcgaccc ctggcgtcac gcgttcggcg gcggcgcgat cggcgcgccg
      961 gctatggcgc ccgaccggta acacgcctcg accagagcca tggtgcgcca ggcgctttcg
     1021 accgacgtct ccatgatcgc gtcctcgccc gccgcatagc gctgcacatt ggccatcacg
     1081 ccgatgaacg cgtcgggaaa ccagccgccc gccagcggaa cctgctcagc ctcttcgccg
     1141 cgcttggcga tccacaattc gtccggctcg ccggtcggat agttcagcag gaggccgagc
     1201 ttgaccatcg cggcgccttg atcgccttcg aagcggaagg tcgcgtcctg gaaccggcgg
     1261 ccgaaatcgt ggtgatggtt gatgctgagc gcgacgcgca gatcgccgcc gtaatcgaga
     1321 atcgcggtcg tgcgcgtatc ggtcagatcg ctggacggat ggccgtagct gcgcgccaaa
     1381 gcgcctttcg gcaggccgac caggctttgg atcagatcga gatagtggat cgaatgcgag
     1441 acgatctcga cacgcggatt gaccttgaga tgggggaaca agccccacgg cgtgacgaga
     1501 ttgaggtgaa cctcgatgtc gatcagccgc ccgagcagtc ccttggcgag cgcgtcgcga
     1561 agcgcgagca tcattggcga gaagcggagc tggaaattga ccgccgcctt gaaccgccgt
     1621 tcgcggcaaa gccggagcgt cgccgtcgct tcgtcgagat ccatgcccat cggcttctgg
     1681 atgagcacgg ccgcgccttc cggcagtgcg gcgagaatgt cgcgatgggc gaccggcggc
     1741 gcggcgatgt cgaagacgac gccgctttgc ttcgccgcct cgccgaggct ggcgaaaact
     1801 ctcggaacgc cccattgcgc cgcgagcgcc tcggctttcc cccggtcgag atcgaaaagc
     1861 ccggcgacgg gaaagccggc cttgcgatag gccggcagat gcgcgtcctt gacgatgccg
     1921 ccggcgccga tgatcacgat cggcctgggg ctggacggct tcggccattc ctggcgcaga
     1981 gacggaagac tcattgcagc acgcctttga ttttggcttc ggcgtcggcg agaagatcgc
     2041 gactcggggt cggcgacgcc aaggcccggc tcagcatctc gtcgataata tgcgccatgt
     2101 cggcgaggcg cggatgacgc ggcaattcgc gcgcgaattc gtgcagccga tcgagcttgc
     2161 cgtaatacgg aatgcgcgcg ttcacctcgc gatcgctcca ggtcgagcgc cggacgccga
     2221 tcgccccctc tctcgtcgtc agaaggtcca tggccgggct cgccgcgtgg cgcatgaagg
     2281 tccaggcgag atcgggattg cgtgagcccg aggcgagggt cagcatccag aagacattga
     2341 gcgatacgct gcgcccgccg gccgaaggca gcggcgcgat gtcgatcttg cccttgaccg
     2401 gcccctcgat gctatcgccg agcgcggcga agccgaacca attggtcatc agcgcgatct
     2461 ttccctcgca gaacatcagg ccggatttga cgctgtcgag cgcgcgcgga tcgggcgcca
     2521 tggcggttgc gtcggcggcc agggcccgga tgaagtcgag cgccgcgatc gcctccttgg
     2581 tcacaagatc gggcctgccg tccggcgcga aaggctcgcc gccgcgcgtc cagacatgga
     2641 cgcagaaatc gtagaagctg ttatggccgt ccggaaacag cgccagaacc gtgccgtacc
     2701 gtccggcctc cggcttgtgc agcctgcgcg cgatcgcgac gaactcgtcc caggtcttgg
     2761 gcggagaaag gccggccgca tcgaacaagt cttttcgata gatcagacat tgcggcccgt
     2821 catggtacgg catgccccag aagccgccgg cgaattgctg caggttcagc agcgacgggc
     2881 tccaggcgtc cggaaaatcg ggaatcggcg cgcgccgcaa atgcggcgcg agatcggcga
     2941 gcgcgccggc ctgatgcgcc tcggcgatcc agtcggtgga gagaaaggcg atgtcgaatt
     3001 cgcccttggc gagcgcctgg cgctcgaaca ggcgctcatg cagcggattg agatcgaaga
     3061 cctcgatctc gatccgcgcg tcgacgcccg cgacgcggac gaaatcctgg aactggcgga
     3121 cgatcgccgt ctcgaagggc ggaaacttgc ggacggcgat gcggatcgtt tcggtcatgg
     3181 cgcgccttta tccgttttga gccccgcctg ctcggcgatg gccgcggccg cctgacgaag
     3241 cgccggcagc atcgcctcca tatccggccc ctcggcatat ttgagcgtcg cgatgaccag
     3301 cgccgcccgc accgccgaag ccgtcccgcc gaccagcacg ccgagatcga gaccgccgac
     3361 gaagcgctcg ccctcggcga tctcatagcc gcgcgattgc acggcgtgca cgcgattgag
     3421 gaaggctgtc ctttcctcct ccggccgcga gaagaaattc gtcgcgctgg agaggaacgc
     3481 gtcgcgctcg tccttctcca tcgcgccaag caggatgcgg cccgaagccg ttgagagcgg
     3541 cgaatgcagc gagccgactt cgatcgaaag ccgaatcggc ttcgggcttt cctcctgcgc
     3601 caggaccagg attttatcgc ggtgaatggc gctgagatgg caggtctcac gaacctcgtc
     3661 ggcgagttgg cgcatgatcg gaacggccgc gcgcaacagc gcctcatagg gcgaatgagt
     3721 ccggctcagc tcgaacaatt tgagcgtcag cgcatagccg ccggtcaccg cgtcgcggcg
     3781 gagatagccg cgcgtctcga gcgcggtcag catgcggaac aattcgccgg gctgccggcc
     3841 gagcgcgcgg ccgagttgcg cctgggtcat cggcacggcc tgatcggaaa gatactcgat
     3901 gatgtcgagc gccttttcga gcgccggcac cgcatatttg ctccgccgct cctcgatgct
     3961 cagaaccttt tcggccatca gcgcttatcc ttggagtaag gtccatcggc ggccgagcaa
     4021 acacgattcc ggcgccgggc gcgagggctt taagccgcgc ggcggcggcg cgcgccgccg
     4081 accgtataga tcgccgccgc cgcgacgacc accgtgccct cgatcaaccc ctgatagttc
     4141 gcgccgagat tgagaatgtt gaagccgttg gccagagcga agagcagcag cgcgccggcg
     4201 agcgtcttga ccacgcttcc ggcgccgccg aagagagacg ttccgccgag aatcgccgca
     4261 gcgatcaccg tcaattccgc gccttgcaac gccgtcgggt tgatcgcgcc gaggcggcag
     4321 gcgaacagaa ttccggcaaa tccggcggtg gcgctgcaaa ggacgaaggc cgccatcttg
     4381 tagaggccga ccttgacgcc cgagagccgc gccgcttcgg cgttgccgcc gacggcgtag
     4441 acgcgccggc cgatcgtggt gaagccgagg acgaaccaga cgatcagcgt gtagattgcg
     4501 gtgtagatca ccggcttggc gacggtcagg ccggcgccgc cgagcacgcc gagaaggatc
     4561 aagaccgcgc cgccgatcgc gacgccgata ggagccccgc ccttccgccg gaccatcgcg
     4621 taagccgcgc cggcgatcag cacagcgccg atcgcgaaca gcgccgcctg gatcggcgct
     4681 ctgccgcctt cgaaggcgac catttgcgct ttgaccgccg ggtcctcgac cagaagcgac
     4741 cggctgttgg tgacgatcag gacaaggccg cggatggcgg tcagggttcc gagcgtgacg
     4801 atgaaggcgt tgatgccgag aagctggacg atcgcgccgt tcaagagccc gacggcgacg
     4861 gcgaccagca tggcgacgca aaccgccggc cagaagccca ccgcgccggc aaggctgatc
     4921 agcaccgcgc cggaaagcgc ggcgaccgag gcgaccgaga gatcgaaatt gccggtgatc
     4981 aggatgacgg tcatcgcgac gcccatcatg ccgacaagcg aggcttgata gaggatgttg
     5041 gtcagattgg cggcgctgaa ataattcggc ccgcccgacg aggcggtgat caggccgatg
     5101 accgccatca gcaggatcag cgcatagtag acgcctgcct cgcgaagccc gaaatgccgg
     5161 cgccaatcct tggtgttcat ctcgaagctc aatgaagcgc gacggcggtc ggctcggcct
     5221 gcgcgccgcg cgccccgccg gcaagcagca cgagctcatg gtcggaggtc gcggacgccg
     5281 cccgctcggc gacgatcgcg ccgtcgcgca tgacgaggat gcggtcgcag accgcgagca
     5341 actcctcgaa ttccgaggag acgatgatga tcgcgacgcc ggaggcggcg agacgccgcg
     5401 tcagcgccag gatatccgcc ttggcgccga catcgatgcc ggcggtcggc tcgtcgagaa
     5461 gcagcagctt gacctcgccg aacagccact tggcgacggt cactttctgc gcattgccgc
     5521 cgctgagctc gccgaccagc gtctccggcg acggcgcctt gatgcgcagc ctttctatgg
     5581 cggattcgcc gcgcgcccat tcgacgtcgc ggcgggtcag cagccgcccg acggcggtgt
     5641 cgtcgagatg cggcaacgtg acattgcgcc aaatctcgaa agcggggatc aagccttgcg
     5701 ccttgcgatc ctcagggacc agcgcgacgc cggcgctcac tgcgccggac gtggcgcggg
     5761 gcagcggctt gccgtcgaga agaacttcgc cggtcgcgcc gcgatcggcg ccgaaaatcg
     5821 catgcagcaa ttcgctgcgg cccgagccga gcagcccggc aaggccgacg acctcgccct
     5881 tgcgcacgac gagatcgatc cggcgcagtc tgtcgccgga ggcgagattg cgcacgacca
     5941 gcaattcggg tccggcgacg ggctcgcggc cctcgcgcag atgatcgagc gtcgcgaccg
     6001 ccttgccgac gatcgcctcg gcgatcgcgc gctcgtcgac atccttggtc ttggcgcgca
     6061 tggcttcgcg cccgtcgcgc aacaccgtga cctcgtcggt cagcgcgatg acctcgtcga
     6121 gaaaatgcga gacgaagacg atgcccttgc cctgcccggc aaggcggcgg accgtctcat
     6181 agacgatttc ccgctcttcg ttggcgagcg aggccgtcgg ctcgtccatg acgatgaggt
     6241 cggcgccgcc gcccagcgcc tgcatgatcg cgaccatctg ccttttgccg atcggcagat
     6301 cgccgaccag ggcgtcggga tcgatcgcat agccgacatc gcggcatagc gcctcgaagc
     6361 gggcgcggtc gtccgggttg cggcgccaga ccccgccccg gccgagaaag acattttcca
     6421 gcaccgacaa agtcggcacg agcggaatat gctgatggat ggtggcgatg ccggcggcgt
     6481 tggcctcggc cggcgtctta aacgcgaccg gcgtgccgcg ccacgcgatc gtcccggccg
     6541 acgccgccgc ggcgccggac aggatgttga tcagcgtgga cttgccggcg ccgttggcgc
     6601 cgagcaggcc atggacccgg ccggcatagg cgtcgaaatc gacgcctctg agggcgacgg
     6661 tcccattggg aaagatcttc tcgaccttcc gcaattgaag caagggcgcg tccacggttt
     6721 taaagcttca gagggttcat ttggttgacc gcaagcgtgc gccgtaccct caccccaacc
     6781 ctctcccgca agcgggagag ggggcgcggt cgccgaccgt ggctacaacc cttctcccgc
     6841 ttgcgggaga aggtggcgcg aagcgccgga tgagggtaag ccgcgataga aggcaatccg
     6901 tactgacctc accactgccc gacgcattgg tcgatattgg ccttggtgac gccgggcgtg
     6961 tcgtaggtga tgaattcatg gtggcgtacg ttcttgcccg tcgccgcctc gtagaccgct
     7021 tggaacgcca gatggccgag ctcttccggc ttgtagcaga ccgtgccgtc gaccgagccg
     7081 tccttgatcg cggcgatcgc cagcttcgag ccgccgaagc cgatcagctt ggccttcgag
     7141 ccggcggcct tcacggcccg cgcgcagccg acgaccatgt cgtccgcctg gttgaagaag
     7201 agatcgacgt cgggatgggc ggagaggatg ttctggcacg ccgcctcgcc cttctcggcc
     7261 gtccagtcgc ccggctgcga ggcgacgacc tggtatttgg cgtgggcctt gtcgagcgct
     7321 tcgaggaagc cctggctgcg ctgcttgacc tccgagaagc cggcctggcc ctcgatgatg
     7381 gcgatcttcg ccacgcgatc cttcggcagc agcttgagcg cgagctcgcc ggcgcgattg
     7441 ccggccgcga tctcgtcctg cgtcaccacg gcgacgagct tcggatagac gtccttgatc
     7501 gcccgtttgt tgggatcgcc gatctgcgag ccgaccgagg cgacgggtat gccggccgcc
     7561 gcgaccttgt cgacccagcc ctgggcgacg gtcgaatcga gcggcatgat gacgatccct
     7621 ttcgccttct ccgcgatcag atcgtcgata tcgttcgcct gcttctggac gtcgttcttg
     7681 gcgtcgagca cgacggcggt gacgccggcc tgcttggccg cgtcctgaaa gcccttggcg
     7741 atcgccgccg cgaagggaaa gctcaagccg acattcgaaa agccgtacgt atcggcggcg
     7801 aaagccggcg ccgcggcggc ggcgctcagc gccgcggcca atgtccacgc gcttatgctt
     7861 ctcttcatcg tcatcgtcat cgtccctttc ctcccggttg gctgttccta acggtgagac
     7921 tttcgcaaaa ttcggcccgg cgcggccgct cagcgcgcga ccggatttcg gcgctcgacg
     7981 agatagagat gttcgcaaac cagaaccggc tcgttgcgct gattgacgat ttcgatcgcc
     8041 tcggcgacga ttccaaagcc gttgcgcttg ggatggtcgc gcttctcctt gatcgtcgcg
     8101 ctgacggtga tcgtgtcgcc gatgaaaacc ggcttcaaga agcgcaggcg gtcatagccg
     8161 taggacatgg cgcgcgggtt gattgcgccc gccgtcatgc cgatgccgac gctgaagacc
     8221 agcgtgccat gcgccatgcg ctgcttgaaa tcctgggtcg cgcaccattc ggcgtccata
     8281 tgatgcggaa agaagtcgcc gctctggccg gcatgcagga cgatatcggc ttcggtgatc
     8341 gtgcggccga aagagcggcg cgaaaagccg atcacgtaat cttcgaaata ttgctcagcg
     8401 atcatcggac gctcccgcgg cgccggctag accgaattgg gcgagcacct cctgcgtatg
     8461 ttcgccgagc cgcggcgcgc cgcgcttgga tttcaacgtc ctgccgtcga cctgaatcgg
     8521 gcagcgcgtg gtccgcatcg tctcgccgcc cgagctcgcg acttcctgaa ccgcgtcgag
     8581 cgcggcaaat cccggatgcg tctcgaggcg cggccaatcg aacacttcgg cgcaccagat
     8641 gtcggccggc tccagcagcg cgagccaggc ggcggtcggg cgcgtgcgca gcaaatcggc
     8701 gatgacctgc ttgatcgcgt cgcggtcgcg gtaccaggtc gcctggtcgg catagggcct
     8761 cagcgcctcg cagcttaaaa ggtccgcgag cttgccgatc ggcgtcatcg ccagcgccat
     8821 atagccgtcg gcggtggcgt agacgccata gggcgcggcg gcgtggacat tggcgttgct
     8881 gacgcgcgag cgccgcggct ggccggcttt cgaattcaga taggcggtga acggctcgaa
     8941 ctggagatcg agcgccgagg ccatcagatt gacgtcgacg cgcccgccct tcccggtcgc
     9001 gccgcggcgg atcagcgctg cgagaacgcc ctgaacaaga tgcgcgccgg tgaaaatatc
     9061 cgcgaccgag acgccgaccg gcaccggtcc ttgttccgca tcgcccgaca gccaggcgag
     9121 gcccgacagc gactgcgcga gcaagtcctg acccggcttg tcgcgccacg gcccgccgac
     9181 gccatagccg gtcaccgttc catagacgat gcgcgggttc agcgcggaga cttcgtcata
     9241 gccgagcccc agccgctcca tcacgcccgg ccgaaaattg tggatcatca cgtcggcgcg
     9301 cgcgatcagt tttttgacga gcgcgagatc ggcgggcttc ttcagatccg ccgtaaggct
     9361 tctcttgttg cggttgatcg tgtggaacag cgcgctgtcg ccgtcgaaga cctggtcggc
     9421 gatggtgagc tggcggcaga gatcgccgcc ctgcggccgc tcgatcttga tcacgtcggc
     9481 gccgagatcg gcgagccgga gcgcgcagga aggcgccgcc agaaattgcg cgaaatctag
     9541 aacgacgatc ccctccagcg gcaggcctga attctcggcc tcattcgccg gcgccctcgg
     9601 cgcagtctcg ctctccctcg gcggcatttc ccgacccgtg attagtttat atgagaatga
     9661 tacgttgata taaaaaccaa agtcaatggg cggaccaccg ataatcgccg tttgcgcttt
     9721 ccgcgctcat tcgcgcgcga gcgggaatcc aggcggagct ggcttgagag cgcggcggtt
     9781 ctctggattc gcgcttgcgc gggaacgacg ggagggttgc ttcgcaacgc gcctgcgccg
     9841 aaccgttcag cggtcggcga gccggcgcca aagcgcctcg taattcgcgt cgttgacgcg
     9901 gttggcctga tcgcggccgg ccgtattggg attgaccatc accatcggct tgacgccgcg
     9961 cgcgacgagc ttctcggcgg tcgccgacac gatcgcgtgc gcgatggcga tggtgacgct
    10021 ggtcgagctc gcgccgaccg gatgctccag cccgtcgatg gcgagcgagg cgtcggcgag
    10081 cggcgcatgc gtgtcgatga cgatgtcggc gacctcgaaa agccgcttgc ccgaggaatg
    10141 gcgcgacgcc gtattctgcg aatgcggaag cgaggtgacg gcgatcgtct tgaggccttt
    10201 cgccttgctc gccagcgcga tgtcgaggat caccgcgttg acgccggaat gcgagaacag
    10261 gatcatcgca tcctgcggat cgagccggtg cgcgctcaag atcgccttgc catagccctc
    10321 gacggcgtga atgaagcgat attgccgcgc gcccatatcg ccccagacgc gatggaagga
    10381 gatcatcgta ctctcgacga tcggacggaa gccgacgatg gttccggtgc gcggaaacat
    10441 ttcgagcgcc ggcagcgcgc catggccggt gccgaaggcg aagaccagct tgccggcggc
    10501 gatgctgtcg gccgaggccg aggcggcggc gtcgatcgcc gcctcctggc tcgtccgcac
    10561 cgcttcgagg cggtcgatca gggtcttgaa atagcggtcg atcagcgcgt tcacgggctt
    10621 caatcctctt tacggcttgg gcaaggcggc gagcacgtcg agcgcgatct cgaacatttc
    10681 ccgctcgcag attgcttgct cggcttgcgg cagcgtctcc tgggtcagcc cgttgaccgt
    10741 gcccatgcac aggctcgagg cgcgggcgcc gagcagccgc ccgaccgcga gcaaggccga
    10801 cgtctccata tcggtcgcca ggatgcggta gcggacgaga tcatggcgca ggtcgtcgat
    10861 gacgcggcgc tggccgtcgc tcagcgcgaa catctggctg tagaagccgt cataggtcgc
    10921 gaacacgccg gcgcgccagc cgcggccgct ttggcgcaag cgcgcgcgca aggccgcgtt
    10981 gagatcgaaa tcggcggccg ccggatagcc cgcgggcgca taggtcaacg aggcgccttc
    11041 ggcgcgcagc gccgcgtcgg cgagcacgaa atcgcccaag acggcgggcg gcatgacctg
    11101 cgccgtgccg atcctgagga acgtatgcac gccgagatcg cgaagctcat ggagcacgat
    11161 cgtcgcgatc ggcgcgccca tgccgaaggc gaccgcggtg aggcgccggc cctggcgctc
    11221 gccggtcacg gcgcgcaggc cccgcctttc ctccagcatc cgaggggcgt caagcgcggc
    11281 ggcaaggcgc gcgatccggg ccggatcgcc gacgagaatc gccgagtcgc cgacctcgtc
    11341 cgcatggcag ccgatatacc aggccgatct cgtcatcctt cgcctccata cgccgctcgt
    11401 tcgttgcggg acgtcagcat gtcccgcgcc gccgtttcgc cggcccgcac cgccgccagc
    11461 ggatcgttgg gtttcgtcat cagcgcggcg agcaagccgc cgaggaacgt atcgccggcc
    11521 ccggtcgggt cgcgcgccgc cacgcgcttc tccgcttcga tcggcgcgcg gatggttcgg
    11581 ccgctcattg tcagcgtcgc gccggccgcg ccatgcgtct cgacaacgag acggtcggcg
    11641 cgcccgggcg ccgcggcgag gaatggcgcg acgaaacccc gttcgccgtg gctgtgaatc
    11701 acgagatcgg cgcgcgccgc caaatcgcgg cgcaaatcgt ccgggaaggc gttgggatcg
    11761 cccttgaccg cccagatcag atattggcgc tcggaaatcc gctcgagcgc ggcgcgggag
    11821 gcggcggccg gctgcacggt gaggcacacc caatccgccg cctcgatcat cgcgagctgc
    11881 gcggcattgt acgcgccggc ggacgcagcg gcttcgtaga agcaatagca gcgcccagcg
    11941 ggatcgtacg caagaatgca gaccggcgtc gggccgggca ggctgcgcgc gacgccgtcg
    12001 ctcgaaaggc ccagcgcggc gatgtgccgg atatagtccg agcccgcgag atcggcgccg
    12061 atccaggtga tcagcgcgat atccgcgctc ccggcgcgcc tgatcgcggc gccgatatag
    12121 gtcgggctgc cgcccggccg cggccacgcg tcggcggcgc gatgggtgac gagcacggtg
    12181 cggcccggct gcggcgcgcc ggcgagttga acgacataat cgaggctggc gtagccggaa
    12241 atcgcgatgc gggtcatgcg agcgccttca gcgcctcgtc gagcgagacg actttgccga
    12301 aatagcggtc gatgtcttcg agcgacgcgg cggcaagatg cggccggttc gaattggtcg
    12361 cttcgcgcgg cagtagaacc tcgaagccat gcgcaaaggc gtcctggatc gtggcgcgaa
    12421 tgcagacatt ggttttgacg ccgccgacaa tcagccgccg cacgcgctgc tcgttgagaa
    12481 agagcgcaag gtcggtggcg aaaaacgcgc tgtagcggcg tttcggcgaa acgatctcgc
    12541 gcggccctgc cgggccgaag ccttcgacga aagccgaatc ctcgtcgccg atgaaatgat
    12601 gcttcggcag cttgcgccat tcgaaatcgt cgaaggcggg ataatgccgc tcgaccgcgt
    12661 gaacaacgat cctgtcgctg gcgcgcgccg cggcgagaag gcggcgcagc ggatcgagcg
    12721 ttgcggccgc ttccggatag tggttcggca taccggggcg gaagaaggag ttgatcacgt
    12781 cgatcaggat caaggccgtc tcgccagacg agtccatggc tcacgctcct tgccacaagc
    12841 gccgcctgcg ctcgccaaga ccgatcgcgg tcaggacgac gagcacgacg atatagggca
    12901 gcatctgcat cagctcaggc gcgacgccgc gcccttgcag atagatttgg atggcgtcga
    12961 acaggccgaa caaagccgcg ccggcggcgg tcaggccggg ccggttgcgg ccgaaataga
    13021 aggcggcgag cgcgatgaag gcgcggccgc cggaaatgcc gacgctgaac acgccgacca
    13081 cgccgatcga caattgcgcg cccgccaagc cggccagcgc gccggcgacg gcggtcgagg
    13141 cgtcgcggat cgcgagcgga tcgaggccta gcgccctcgc cgcttcgggc gccgcgccgg
    13201 cggcgcgcag ccgtaggccg gcgcgggtgc gcgacaggac gaaggccgtg atcggcacga
    13261 gcgcccaaga gagccaagtc aaaggatcct tgcccgagag gatcgcgccg acgatcggaa
    13321 tttgcgccac gcctggaatg acgatccgcg gcagcatgac gacatccggc agccggatgg
    13381 tgccggaact gtggtaatag tccttcagga cgaagccgat caggccggcg gccgcaacgt
    13441 tcaagccgac gccggcgacg atttcattgg cttgcagacg cgtcaccgcc agcgaaaaca
    13501 agtagccgac cacgcctccc gcgacgagag cggcggcgag cgcgagcggc caggattgcg
    13561 tcgccgagga cgcggcgagc gcgacatagg cgcccgtgag catcatcgcc tccaggccaa
    13621 gattgacgat gccgccgagc cggttgatga ggccgccgat cgcggcgagg agaatcggcg
    13681 tcgtcagcaa cgccgccgcg gcgagaacgt tgtcgatcat gccgcgctcc gcccgcgcag
    13741 cagccggccg aagcgcacga ccgcgaaaat cgtcaccacg ccttgcagca cgtccacgat
    13801 ctcgagcgga atatcgctga agagctgaac cgtggttccg gccgacgcca aggcgccgaa
    13861 cacgatcgcg ccgatcagca tgccgatcgc cgaattgcgt cccatcagcg cgaccgctag
    13921 gccggtccag ccgtaaccgg gcgaaaagcc gacgacgaag cggtgcgtca cgccgagcgc
    13981 atgggcgctg ccggcgaggc cgccgatcgc gcccgaaatg atcatcactt tgaggatgag
    14041 gcgcggcgcg tcgatgccga tggcgcggct gaagcgcgga ttgaggccgt tgagccgggc
    14101 ttcgaagccg agcggcgtca gccggtccca gaccgcgtac aaggccagca aggccaaagc
    14161 gatgataaag ccgaggctca aggtcgaggg cggcgcgatg cgcggcagcc agcccgccgc
    14221 ggcgatcatc ggcgtcgccg aattggccga gcctggcgcc aggaacgggc cgttcacaag
    14281 gaacaatgtg agattcaccg cgacgaagtt gagcatcagg gtcgagacga cctcgtccac
    14341 ttcgagccgc gcccgcagca aagccggcgc ggcaagccac aggccgccga cgagcgcgcc
    14401 cacggcaagc tccgcgacga tcagcagcgg cgacggcaaa gcggtcaagg tgaagccggc
    14461 cacggccccg gccagaccgc cgagagagaa gacgccctcg acgccgacat tgaatatgcc
    14521 ggcgcggaac gaaatcgcgg tggcgatcgc cgtgaacagg atcggcgtcg ccgcagtgag
    14581 cgtcgcggcg acgcggcttt cgccgccaaa tccctcgcgc agcatcaggc cgtaaacggc
    14641 gagcggattg cggccggtcg cggcgagaag gattgcgccg acgatcagcg cgaccgcgaa
    14701 gggcgcgagg gccgcggcga gcgccgccca atcgaggcgc gatgttcgca cgacgcctgt
    14761 cgtcgccgcc gtcacgccgc ttctccaagc atcatgcggc cgatccggcc aacgctgcga
    14821 tcggcgccgg cgacttcgcc gatcacggcg ccgcggtaga ggacgaggat gcggtcggcg
    14881 agcgtcagca attcctcgat ctcttccgag acgaggagca ccgcgccgcc ggccgcggcg
    14941 aaagcgagga tctggcgatg gatgaaagcc gcgccggcaa tatcgacgcc gcgggtcggc
    15001 tgcgccgcga ccaggacgcg cggattgcgg tcgagctcgc gggcgatgac gacgcgctgc
    15061 tgattgccgc cggaaagcgt gcgcgccgcc tcctgacaat cgcgatagac aaccgcgaag
    15121 cgcgccagcg ccttgttcgc atgcgtacgg atcgtcttcg gccacagcaa gccgcgccgt
    15181 gcgaaaggct tggcgcgatg ccggccggcg atcacattgg cggcgatcgt ctcggcgaga
    15241 cagagcccct catgggcgcg gtcggcgctg atatagccga gccctgccga gcggcgcgcg
    15301 gcgaccgaca atgcggtgac gtcccgcccg gcgatcgcca cccgcccggc gatcgcccgt
    15361 cgcaagcccg tgatcaccgc gacgatctcg tcctggccgc tgccgctgac gccggcgagg
    15421 ccgacgatct cgccgggacc gatatcgaga tcaatcccgg tcaaccgctc gacgccgcgc
    15481 gaatcgagcg ctctgacgcc gcgcaaagag agagccgact tctcggacga tcgcgtctgc
    15541 gccaagcggc ggggaaattc gatcgtctcg ccgaccatca actcggcgag ccggccgata
    15601 ttcgcctcgg cgggaatcgt gctggcgacg accttgccgg cgcgtaaaac cgtgatcgca
    15661 tcgcagaccg tgagcacttc gtcgagcttg tggctgatga agagaatggt gcggccttcg
    15721 tcgcgcaagc ggcgcaacag attgagcagg tcgcgcacct gctgcggcgc gagcacggcg
    15781 gtcggctcgt cgagaatcag gacatcggcg ccgcgataga gcaagcgcag gatttcgaga
    15841 atttgccgca catggaccgg cgcctccgag actctgaggc gccagtccaa atcgacgccg
    15901 gccgacgcgg cgagccggcg cgcctcggca agcgcccgct cgcgatcaat gacgccgcct
    15961 gggccgaccg gctcgcgccc gagaatcagg ttctcgagca gcgtcaaatc ctccaccaac
    16021 atgaattcct gatggaccat gccgacgccg cgggcgaagg cgtcggcggg atcggcgagc
    16081 cggaccggct cgtcgttgag gatcaccgcg ccttcgtccg gctgttcgag gccttgcagg
    16141 atgcgcatca gcgtggtctt gccggcgccg ttgccgccga ccaggccgcg gatctcgccg
    16201 ggcgcggcgg cgaaatcgat cgcgtcattg gcgataacgc cgccgaagcg gcgcgtgacg
    16261 ccgcgcgcgc cgatccggac ggccggcttg atcacctcgc gctccttagg ccgcgcttac
    16321 tgcttccacc agctcggata gccttgcgtg gtgacgtccc agaccttgat ctcgccggca
    16381 atgatcttct tcttcaggtc gtcgatcttg gcgagatcgg cggcgggaat caggtctttc
    16441 gtgtaggtca tcggcgacag gccgacgcca tcctctttca agccgagcgt cacggtttgg
    16501 ccgccgggga atttatccgc cgcataatcc ttgatcaccg tctcgacggc gagatcggtg
    16561 cgcttgatca tgctggtcag cacgtggccc ttggcgagcg cgtcctggtc ggtatcgacg
    16621 ccgatcgcat aatgcccggt ctccttggcg gcctggatga cgccaatgcc ggtgccgccg
    16681 gcgacctggt agacgatgct cgcgccttcg tcgaacatgg ctttcgccat ttgatggccg
    16741 atcgccggat cgccaaaatt gttcgaatag gcgaccttga cctcgacttt cggatcgatg
    16801 tcatgcgctc cctgaatata gccggcgatg aacttgtcga tgccggccga ttttgtgccg
    16861 ccgatcacgc cgatgatggg cttcggcccg attcccttga ttttcgggtc ggaagcgacc
    16921 atcgcagcga gcgctccggc gaggtacgag ccctcctgct ccttgaacag gaccgaggcg
    16981 atattggcgc ccggctcgac cagattgagg atcgagaaat cgaccttggg gtaatccttg
    17041 gcgacggtct tgatcgcatc gccatgcgaa tattcgagat cgacgatcag gccgaagccg
    17101 ccgtccgccg cgcggcgcag catgttctcg ccctgcgcga caacatcttt cgactcgacc
    17161 tccttgcctt cgagcttggt ctcggcgagc gccttcttga agcctttgtc ggcgagatcg
    17221 ttataagact gatcgccgag gccgccttgg gcgatgatca gcgccaccgg cgccttctca
    17281 gccgcctgag cgacgggcgc cgcgaagctg aggaccaaag cgagggccga aacgctgagc
    17341 gccaggcaag acttcttcag gtacgacttt cccatctcca ctctccttca tggccaaagc
    17401 gggaggaatc ccgcggtgcg gatgccgcat cagtagcgcc tggtagctgt agtgccggca
    17461 atcgtggatg gccgcgacat ggtccagaag ccgaccgtcg acgccatagg tctggcgctg
    17521 gatgaccatc acgacgtcgc ttgcgccgag gcgtaaaagg tcgcgctcct cgggcttggg
    17581 caggcgcgct gtgaggacct cgacgcaatg gtcgagggcg aggccggcgc gctccatttc
    17641 gcgatagagc gagaaatccg gcctgccgac gagcgaggcg ttcagtccca attcgtcgct
    17701 gaccgattgc gagagaaccg aatggtggat ggcgacgggc gcgccgtcgg cgaaacgcag
    17761 gcgatccaag gcgatgagcc cttgatcggc gggatagggc gcctctggcg tccaggcgac
    17821 cggcccgaaa ccgatgagtt cgctgcgcgg ccgccggccg gcggcgacaa cggaatcggt
    17881 gaagcttcgc aattggccgg gcagccgttt caacggctgg gcggcgacga aagtgccctt
    17941 gccttggcga cggacaagaa gtccttcgtc gaccaggcct tcgatcgcct tggcgacggt
    18001 gaaccggctg acattcaggc gttgggcgag ttccggctcg ctggcgatgc ggtcgcctgg
    18061 cttgagcgcg gcgatttcgg cgcgcaactc gtccgagagg cggcgataga gcggccgccc
    18121 gtcatcgact ttgagaatgt gcatcttcgg acctgcccaa cggacatccg gttgtccgga
    18181 caatacgtaa cgattgaggg cctggcaagc tgtaggcgcg gtcgctcacc gcgccgcttc
    18241 cgccgcgctt tctaaaggtg aaacagcacg cttcgcgtcc gccgcggtca gcgaccgcgg
    18301 ctacaagtcg actcgctcct cgcgatgttg gctggcgtga aggacggcga atgtccgtgc
    18361 gtaccctcac atggccgccg ctcacacttc cggcaggttc tccttgcaga taatctgcag
    18421 gcgcggcgca tgatattcgt agggggcgcc tctgacgctg tgggtcagga tgttcagcgc
    18481 ctctctcgcc tcgacccgcg ggttctgatc gatcactgcg tcaagcgttc cgtcgagcag
    18541 cagcgccttg ttggcgtcgg tcagctcatg cccgacaaaa accacctctt tggcgcgact
    18601 cctgtcacgc aaggcgcgcg caatgccctc ggtcccggcg ccgatattgt agacgccggc
    18661 aatgtcgggg tggcgcttga gaagattgtc cgcctcgacg aaggctttct cgaaatcgtc
    18721 cttgacctcc cgaagctcga ccacttcgag attggcgaat tcctcggcca ggatgtgcct
    18781 gaatcccatt tcccgctctt catggccgcg ataggagagc gagcccgcga agagcgccac
    18841 cttcttgcgc tccgaattgc cgaggaaacg cccgagaatg tatcccgcga cgcggcccgc
    18901 ggcgcgattg tcgatgccga cataggcgac gcgcggcacg tgcgaaatat ccgaggcgag
    18961 cgtcagcacc ttgacgccgg cggccgacaa ggcgcggatc gcttcgcgca cggtcggatg
    19021 atcgagcgcg atcacgccga cgccgcggct cttgccgg
//
LOCUS       DATGJW010000210         6078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1387537, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000210 DATGJW010000000
VERSION     DATGJW010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6078
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1387537"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1344
                     /locus_tag="VKU03_06665"
     CDS             <1..1344
                     /locus_tag="VKU03_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70981.1"
                     /translation="GDRARNAGIKIQNPSMGWALPGFYLLGREWLINLGLIRPQDAVE
                     DLIYVMDFWKRFQLSWHRNNGHITNKEFGHRGQFLPERRIQVFHADMYDCKQGDALHE
                     AAQAFMAAASQYGFLISCESRISLHNSGPYKLADDREMIVRDFMDLSESDFPWLDDVA
                     RDVPYNNLTVAMAVKDCHFYLVDDWGSFESKPEFAADKLVGVGLYTSDSLSDGYRPVA
                     MGSREELTNCFRSLTEKVKDANNKLWRRVAGWSRDQMMDAGAITYFSIVKDLAHVAGV
                     YEVADWMMVDERAERFRPLFNDEYSRDALVGLCIGAHPSHRLSEYTMMQHSNAPQRLY
                     SLIPYSILQGEDYTPTSGPIYPGASHLPAKLDRYNTTSGVIGLAEYNRLAREARPPAL
                     EPKYRHLCETWVKYHAGEPLADELYRLDQANSRLLKDKGSRLKRADVEKLRASSR"
     gene            1362..2867
                     /locus_tag="VKU03_06670"
     CDS             1362..2867
                     /locus_tag="VKU03_06670"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLJ70982.1"
                     /translation="MQIGSLIRRAALHYGAAPCLAEGGRVVSFREFDALTDRLGNALL
                     ASGFAPGDRVGVLLPNGVDCLVVYYALAKSGLVRVALNARETLDNHAFKLRDSGSRGV
                     IHNLTSDADIARLGVERPIGPAELQALAASGADAPCAVDRALDAPYRLGYTGGTTGRS
                     KAVTLTTRGELAELSAFLTDLTPDLRRGETFLHAAPIAHASGAFFLPSLTRGLRSLVM
                     AKFDPAEFVALAARERCEHTFLVPTMLAMALEVAGVEEAEFALRSISYGASPIAPALL
                     KRAEKRFGRIFAQTYGQAESPMVITCLKPEDHDRVGSCGRPFTIVDVAVFDEQDRPLP
                     PGERGEIVCRGPQTMAYYWNNPEATAQAFRNGWLHTGDLGYMDSDGFFYLVDRKNDLL
                     ISGGYNVYPREVEDVLLACDGVIEAAVIGLPDEKWGERVYAVVAGRSGLDADRVMAHA
                     RDKLASYKRPKGIEIWPELPKTSANKILRRAVRDRVVAAMKENASAGKEKA"
     gene            2869..3471
                     /locus_tag="VKU03_06675"
     CDS             2869..3471
                     /locus_tag="VKU03_06675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ70983.1"
                     /translation="MNDAHLVMHGLAIKKHATPEDVAGILGLDVEAVRETLAKLVAGK
                     RAVEAQGRYLLAPAARMLLDSDYSRLYEDVRADPAFQAGYGAFERLNPLLKQLITEWQ
                     TIAAGSERVPNDHSDKEYDAKIIDRLGDFHERAEAALDQLASAVPRMRIYRDKLAAAL
                     DKAEQGAIAWVSDAKIESYHTLWFELHEDLLRLMRRERQE"
     gene            3481..5268
                     /locus_tag="VKU03_06680"
     CDS             3481..5268
                     /locus_tag="VKU03_06680"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: protein motif:HMM:NF004531.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HLJ70984.1"
                     /translation="MTVAFGQWTLGLDGEGEPPRALIGGKAWSLARMQALGLNAPPAF
                     VVTTAACKAYLAAGAPPAGLNEELEAAIAWLEEKTGRRFGEGPRPLLVSVRSGAPVSM
                     PGMMDTVLNLGVNDAAEAALAAECGDRAFARNAHKRFLDLYAHIVLRASAPEFDEAQD
                     PAAWRAAIRANCGAEVPVGPREQLRGAVRAVFESWNSRRARRYRQHNHIPDDLGTAVT
                     VQAMAFGNLDQRSGTGVLFSRNPSTGAPEPYGEYLPRAQGEDVVSGKYTPQPLSALAA
                     VMPEIHAELLAAARKLERAGRDVQDIEFTIERGRLYFLQARAAKLAPQAAVRIAVALA
                     REGVIDQQAALRRLTPDQARLLLSPRIREAALRNAELLAAGEAASPGVGIGVVVGDSD
                     EAERRARAGEAVILARPTTSPEDLHGMIAARAVATEQGGGTSHAAVVGRALGLACVVG
                     CGKGALEPLAGKLVTVDGRAGKIYAGALAVETPNESEDEALATFAAWAAKLSPLRVVA
                     PTSPEADEAVDLSASEDAADPDKIGAVLAALHGARGARGGAVASDQGVRAALRARLEF
                     IVADTVLPPLLAAIRAADAASIAEEVGGT"
     gene            5265..5759
                     /locus_tag="VKU03_06685"
     CDS             5265..5759
                     /locus_tag="VKU03_06685"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HLJ70985.1"
                     /translation="MSEIKPQDIEALLETFEASSWSEMRVKVEGLELFASKTPGARPA
                     MLGETLTGAPAAPAPPPVLSAEPRPPADGGALRAGAIPDGWVAVRAPNLGTFYRSPKP
                     GAPPYVKVGQRVEPTTELCLIEVMKLFTSVTAGVSGVVRQVLVEDAELVEFDQALFLI
                     EAHA"
     gene            5759..>6078
                     /locus_tag="VKU03_06690"
     CDS             5759..>6078
                     /locus_tag="VKU03_06690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HLJ70986.1"
                     /translation="MRLRRVLIANRGEIALRIVRAAKSLGLETVLAASAADRDSLAAR
                     EADRVVTLGPAPARASYLNANLVVHAAKAAGCDALHPGYGFLSERAVLARLCEEHGIA
                     FVGPT"
BASE COUNT          957 a         2127 c         2041 g          953 t
ORIGIN      
        1 ggcgaccgcg cccgcaacgc cggcatcaag atccagaatc cgtccatggg ctgggctttg
       61 ccgggcttct acctgctcgg ccgcgaatgg ctgatcaacc tcggcctcat ccgcccgcag
      121 gacgcggtcg aggatctgat ctatgtcatg gatttctgga agcgcttcca gctctcctgg
      181 caccgcaata acggccatat caccaacaag gaattcggcc atcgcggcca attcctgcca
      241 gagcgccgta tccaggtctt ccacgccgac atgtacgatt gcaagcaggg cgacgcgctg
      301 cacgaggccg cgcaggcctt catggccgcc gcctcgcaat acggctttct gatctcctgc
      361 gaaagccgca tcagcctgca caattccggt ccctacaagc tcgccgacga tcgcgagatg
      421 atcgtccggg acttcatgga cctttccgag agcgattttc cctggctcga cgacgtggcg
      481 cgcgacgttc cgtacaacaa cctgaccgtc gccatggcgg tgaaagactg ccatttctat
      541 ctggtcgacg attggggcag cttcgaatcg aagccggaat tcgccgccga caagctggtc
      601 ggcgtcggcc tttatacgtc ggactcgctc tccgacggct atcggccggt cgcgatgggc
      661 tcgcgcgagg agctgaccaa ttgcttccgg tcgctgaccg agaaggtcaa ggacgcaaac
      721 aacaagctct ggcggcgcgt cgccggctgg tcgcgcgacc agatgatgga cgccggcgcg
      781 atcacctatt tctcgatcgt caaggacctc gcccatgtcg ccggcgtcta tgaggtcgcc
      841 gactggatga tggtggacga gcgcgccgag cgcttccgcc cgctgttcaa cgacgaatat
      901 agccgagatg cgctggtcgg cttgtgcatc ggcgcgcatc cctcgcaccg cctcagcgaa
      961 tatacgatga tgcagcactc caatgcgccg cagcggctct attcgctgat tccctattcg
     1021 atcctgcaag gcgaggacta cacgccgacc agcggcccga tctatcccgg cgcgagccac
     1081 ctgccggcga agctcgacag gtacaacacg accagcggcg tgatcggcct cgcggagtac
     1141 aaccgcctgg cgcgcgaagc tcgtccgccg gcgctcgagc cgaaatatcg gcatctttgc
     1201 gagacctggg tcaaatacca tgccggcgag ccgctcgccg acgagctcta taggctcgat
     1261 caggccaatt cgcgcctgct caaggacaag ggctctcggc tgaagcgcgc cgacgtcgaa
     1321 aagttgcgcg cctcttcgcg ctgacgggca agggcggcga catgcagatc ggaagcctga
     1381 tccgccgcgc cgcgcttcat tacggcgccg cgccctgcct ggcggaaggc ggccgcgtcg
     1441 tctcgttccg cgagttcgat gcgctgacgg accggctcgg caacgcgttg ctggcgagcg
     1501 gatttgcgcc gggcgaccgc gtcggcgtcc tgctgccgaa cggcgtcgac tgcctcgtcg
     1561 tctactatgc gctcgccaaa agcggtcttg tccgcgtcgc gctgaacgcg cgcgagacgc
     1621 tcgacaatca cgccttcaag ctgcgcgaca gcggcagccg cggcgtcatc cataatctga
     1681 cctcggacgc cgacatcgcc cggctcggcg tcgagcgccc gatcgggccg gccgaattgc
     1741 aggcgctcgc cgcttcgggc gcggatgcgc cttgcgccgt cgaccgcgcg ctcgatgcgc
     1801 cgtaccggct cggctatacc ggcggcacga ccgggcgctc gaaagcggtg acgctgacga
     1861 cgcgcggcga gctcgccgag ctctccgcct tcctcaccga tctgacgccg gacctgcgcc
     1921 gcggcgaaac cttcctgcac gccgcgccga tcgcgcacgc ttcgggcgcg ttcttcctgc
     1981 cgtcgctgac ccgcggcctg cgctcgctgg tgatggcgaa attcgatccg gcggaattcg
     2041 tcgcgctcgc cgcccgcgag cgctgcgagc atacgttcct cgtgccgacc atgctcgcga
     2101 tggcgcttga agtcgccggc gtggaggaag cggagttcgc gctgcgctcg atcagctacg
     2161 gcgcctcgcc gatcgcgccg gctttgctga agcgggcgga gaagcgattc ggccgcatct
     2221 tcgcccagac ctacggccag gccgagagcc cgatggtgat cacctgcctc aagccggaag
     2281 accacgatcg cgtcggctcg tgcggccggc cgttcacgat cgtcgacgtc gccgtgttcg
     2341 acgagcagga ccggccgctg ccgcccggcg agcgcggcga gatcgtctgc cgcggtccgc
     2401 agaccatggc ctattattgg aacaatcccg aggcgacggc gcaggccttt cgtaatggct
     2461 ggctgcatac cggcgatctc ggctacatgg attcggacgg gttcttctac ctggtcgacc
     2521 gcaagaacga cctgctcatt tccggcggct acaacgtcta tccgcgcgag gttgaggacg
     2581 tcctgctcgc ctgcgacggc gtcatcgaag cggcggtgat cggcctgccc gacgagaaat
     2641 ggggcgagcg cgtctacgcc gtcgtcgccg gccggtccgg cctcgacgcc gacagggtca
     2701 tggcgcatgc gcgcgacaag ctcgccagct acaagcgacc gaaagggatc gagatctggc
     2761 cggagttgcc gaagacgtcg gccaacaaaa tcctgcgccg cgccgtgcgc gaccgcgtcg
     2821 tcgcggcgat gaaggaaaac gcctcggcgg gaaaggagaa ggcctgacgt gaacgacgcg
     2881 catctcgtca tgcatggcct cgcgatcaag aagcatgcga cgccggagga cgtcgccggc
     2941 attctcggcc tcgacgtgga ggcggtgcgc gagactctcg ccaagctcgt cgccggcaag
     3001 cgcgcggtcg aggcgcaggg ccgctacctg ctcgcccccg ccgcgcgcat gctgctcgat
     3061 tccgactatt cgcgcctcta cgaggatgtg cgcgccgatc ccgcctttca ggcgggctat
     3121 ggcgctttcg agcgcttgaa cccgctgctg aagcagctca tcaccgagtg gcagacgata
     3181 gcggccggca gcgagcgcgt gccgaacgat cacagcgaca aggagtacga cgccaagatc
     3241 atcgaccgcc tcggcgactt ccacgagcgc gccgaggcgg cgctggatca gctcgcgagc
     3301 gcggtcccgc gcatgcgcat ctatcgcgac aagctcgccg cggcgttgga caaagccgag
     3361 cagggcgcga tcgcctgggt cagcgacgcc aagatcgaga gctatcacac gctctggttc
     3421 gagctgcacg aggacctatt gcgcctgatg cggcgcgagc gccaggaata gggcgcgttg
     3481 atgacggtcg ccttcggaca atggacgctt ggtctcgacg gcgaaggcga gccgccgcgc
     3541 gccttgatcg gcggcaaggc atggtcgctg gcgcggatgc aggcgctcgg cctcaatgcg
     3601 ccgccggcct tcgtcgtgac cacggccgca tgcaaggcct atctcgccgc cggcgcgccg
     3661 ccggccgggt tgaacgagga gttggaggcg gcgatcgcgt ggctggagga aaagaccggc
     3721 cgccgcttcg gcgaaggacc gcgcccgctg ctcgtctcgg tgcgctccgg cgcgccggtc
     3781 tcgatgcccg gcatgatgga caccgtgctc aatctgggcg tcaacgacgc ggcggaagcg
     3841 gcgctggcgg ccgaatgcgg cgaccgggct ttcgcccgca acgcgcataa gcgctttctc
     3901 gatctctacg cccatatcgt gctgcgcgcg agcgcgcccg aattcgacga ggcccaggac
     3961 ccggccgcct ggcgcgcggc gatccgcgcg aattgcggcg ccgaggttcc cgtcggcccc
     4021 cgcgagcagt tgcgcggcgc cgtccgcgcg gtgttcgaat cctggaattc gcgacgcgcc
     4081 cgccgctatc gccagcacaa tcatattccc gacgatctcg gcactgcggt caccgtccag
     4141 gcgatggcct tcggcaatct cgatcagcgc tcgggaaccg gcgtgctgtt ctcgcgcaac
     4201 ccgtccaccg gcgccccgga gccctacggc gaatatttgc cgcgcgcgca gggcgaagac
     4261 gtggtgtccg gcaaatacac gccgcagcca ttgagcgcat tggcggccgt gatgcccgaa
     4321 atccatgcgg agctccttgc cgccgcgcgc aagctcgagc gcgccggccg cgacgtgcag
     4381 gacatcgaat tcacgatcga gcgcggacgc ctctatttcc tgcaggcgcg cgcggcgaag
     4441 ctcgcgccgc aagccgcggt ccgcatcgcc gtcgcgctcg cgcgcgaagg cgtcatcgac
     4501 cagcaagcgg cgctgcgccg cctgacgccc gatcaggcgc gtctcttgtt aagcccgcgc
     4561 atccgcgaag ccgccttgcg caacgccgaa ctgctcgccg cgggcgaagc cgcgtcgccg
     4621 ggcgtcggga tcggggtcgt cgtcggcgat tccgacgagg cggagcggcg ggcgcgggcg
     4681 ggcgaagcgg taattctcgc gcggccgacg accagccccg aagatctgca cggcatgatc
     4741 gccgcgcggg cggtggcgac cgagcagggc ggcggcacgt cgcacgccgc cgtggtcgga
     4801 cgcgcgctcg gccttgcttg cgtggtcggc tgcggcaagg gcgcgctcga accgctggcc
     4861 ggcaagctcg tcaccgtcga tgggcgggcg ggcaaaatct acgccggcgc gctcgccgtg
     4921 gagacgccga acgaaagcga ggacgaagcg ctggcgacgt tcgccgcctg ggcggcgaag
     4981 ctctcgccgc tgcgcgtcgt cgcgccgacc tcgcccgaag ccgacgaggc cgtcgatctc
     5041 tccgccagcg aggacgccgc cgatcccgac aagatcggcg ccgtgctggc ggcgctccat
     5101 ggcgcgcgcg gcgcgcgcgg cggcgcggtc gcttccgatc agggcgtgcg cgcggcgctc
     5161 cgggcgcgtc ttgaattcat cgtggccgat accgttttgc cgcctttgct cgccgcgatc
     5221 cgcgcggcgg atgcggcctc tattgccgag gaggtcggtg ggacgtgagc gagatcaagc
     5281 cgcaagacat cgaagcgctc ctcgagacgt tcgaggcctc gtcctggagc gagatgaggg
     5341 tcaaggtcga ggggctggag ctgttcgcat cgaagacgcc aggcgcgcgc ccggccatgc
     5401 tcggcgaaac gctgaccggc gcgccggccg cgcctgcgcc gccgcccgta ctctccgccg
     5461 agccgcgccc gccggcggat ggcggcgcct tgcgggctgg cgcgattccc gacggctggg
     5521 tcgccgtccg tgcgcccaat ctcggcacgt tctatcgctc gccgaagccg ggcgcgccgc
     5581 cctatgtcaa ggtcgggcag cgcgtcgagc cgacgaccga gctctgcctg atcgaagtga
     5641 tgaagctgtt cacctcggtg accgccggcg tctccggcgt ggtgcggcaa gttctggtcg
     5701 aggatgcgga attggtcgaa ttcgaccagg cgctgttcct catagaagcg cacgcctgat
     5761 gcgcctgcgc cgggtgctca tcgccaatcg cggcgagatc gccttgcgca tcgtgcgcgc
     5821 cgcgaaaagc ctcggcctcg aaaccgtcct cgccgcctcc gccgccgacc gcgacagcct
     5881 cgccgcgcgc gaggccgatc gcgtcgtcac gctcggcccg gcgccggcgc gcgcctccta
     5941 cctcaacgcc aatctcgtcg tgcacgcggc caaagccgcc ggctgcgacg cgctgcatcc
     6001 gggctacggc ttcctctccg agcgcgccgt gctggcgcgc ctttgcgagg agcatggaat
     6061 cgcgttcgtc ggtcctac
//
LOCUS       DATGJW010000211         7948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1076435, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000211 DATGJW010000000
VERSION     DATGJW010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7948
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1076435"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..816
                     /locus_tag="VKU03_06695"
     CDS             <1..816
                     /locus_tag="VKU03_06695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018497470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HLJ70987.1"
                     /translation="GDLFARVAQRSYPALRGGTEETTSGRARLAPLREAIKVPVLVIN
                     DSPIKQFAENRHAVGQSLFESYLRFTNRSTNGKRATVFGYGACGRGVAACFRNAFSAV
                     SVVDANPVTTLEAHLDGFATPLREAAIRGADVLITVTGAKDVLTAADLPLIKNGAILM
                     NGGHLPHEIDVAGLRAHASVIGADLYPSEGIETLRLADGRAIHILGAGHMANLAGPRP
                     LGNSVESMDLGFTLQARCLERVARGGLGQDHCVVPVLADIDAAVASAYLAMNR"
     gene            complement(1472..2497)
                     /locus_tag="VKU03_06700"
     CDS             complement(1472..2497)
                     /locus_tag="VKU03_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan -binding protein"
                     /protein_id="HLJ70988.1"
                     /translation="MALARGRRSEGVNYWPGFVDALSTMLLVIIFLLSVFMLAQFFLS
                     REVSGKDTVLSKLNHQIEELTSLLAMERTAKNEAEQNLAGLSATLQASQQEQQKLQNQ
                     IALNSANADSAGDKASAAANALEAQKQVSTRALAQVDILNQQIAALRRQLAAIEDALA
                     ASEASQRESQAKIADLGSRLNVALAQKVQELARYRSDFFGRLRQILGSRPGVRVVGDR
                     FVFESSVLFDSNRADISVAGRQALDDLAVAVNELAREIPPEIPWILRVDGHTDKHPIT
                     GGPFKSNWELSAARAIAVVQYLITKGVPPDRMAAAGFGEFQPIDQGTDEESLARNRRI
                     ELKLTEK"
     gene            complement(2522..3475)
                     /locus_tag="VKU03_06705"
     CDS             complement(2522..3475)
                     /locus_tag="VKU03_06705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor protein MotA"
                     /protein_id="HLJ70989.1"
                     /translation="MAIESEVRPLTSPRIFLIGMAAFLIIVAFVVLILEKQIETAFHA
                     NPGLNGFIIGVLLLGSFFTFRQVIRLFREIGWVNSLSGARPWRSNKGPRLLGPMATLL
                     NAGVGARGLSTTTSRAILDSVGTRLDEGRELNRYLIGLLVFLGLLGTFWGLLETVRSI
                     GGVINAMQTNVDAGAMFDDLKSGLSKPIAGMSISFTSSLFGLAGSLILGFLDLQAGQA
                     QNRFYTELEDFLASHVETSAPPPPVEMIPDKLPVAYDPAEQRKSAAAMANLAEGIQGL
                     VNHMRQEQQLIRDWVEAQAAQQEEVGKLLRRLAGESETHGM"
     gene            complement(3692..4483)
                     /locus_tag="VKU03_06710"
     CDS             complement(3692..4483)
                     /locus_tag="VKU03_06710"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLJ70990.1"
                     /translation="MIRSALMNVMTSAAIKAGRGLKRDFGEVGNLQVSMKGPGDFVSL
                     ADKKAEKLLFEELSKARPGYGFVMEESGRVEGSDKTHTWHIDPLDATTNFLHAIPMFA
                     VSIALEREGQIVAGVIYNPATDDMYVTEKGQGAFGGNRRLRVANRRELSQALIGCGVP
                     HLGKAAQHPRFKAELAAVMARVVNIRRMGCAALDLAYVAAGLFDAFWERGLNSWDIAA
                     GALLIREAGGFVTGADGEENFLETGSICAGNEIMHREMLALLKDA"
     gene            4610..6280
                     /locus_tag="VKU03_06715"
     CDS             4610..6280
                     /locus_tag="VKU03_06715"
                     /EC_number="6.3.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015930966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004329 - formate-tetrahydrofolate ligase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate--tetrahydrofolate ligase"
                     /protein_id="HLJ70991.1"
                     /translation="MKSDIEIAQAAKLEAIAAVAAKLGVPEAALHPYGRHVAKIDFDF
                     LDSLAERPDGKLILVTAINPTPAGEGKTTTTIGLGDALNLIGKRAAICLREPSMGPVF
                     GQKGGATGGGWAQVAPMVDINLHFTGDFHAIAAANNLLAAMIDNHIHWGNELGVDPRR
                     ILLRRALDVNDRALRQTIAGLGGPANGYPREDGFDIVVASEAMAVFCLATNLDDLQKR
                     LGRMVVAYTREKKPVTADDLKATGAMAVLLKDALRPNLVQTLEGSPAFVHGGPFANIA
                     HGCNSVIATKAALKLADYVATEAGFGADLGAEKFFDIKCRQAGLKPAAAVIVASVRAL
                     KMNGGVGRGALAAENLAALETGLANLGGHVANMRKFGVPIAVAINHFATDTEAELALL
                     QQAARERFGAEAIVCRHWAEGGKGAEALARHVVALADSGAAAFKPLYPDDLPLWDKMK
                     RIAVEIYGASDISASAAARRQFADLEAAGYGRLPICVAKTPYSFSADPSLLGAPSGHV
                     PPIRELRLRAGAGFIVAIVGDVMTMPGLPRRPGAEKIGLAGGVVQGLS"
     gene            6378..>7948
                     /locus_tag="VKU03_06720"
     CDS             6378..>7948
                     /locus_tag="VKU03_06720"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ70992.1"
                     /translation="MHFLLNRAKRFAVMAHDLATTPLAIALALYLSFPANARLHTEAL
                     AWIVPLFTLYAGGVFRFFRLYEAKWRFASLPDLFNIFRAASTLAISLLVVDLAIVARG
                     VADGFVFGEKVCFLYWVLQLMLLGGPRIAYRYWRYVQARRTGRRERAPSILVLGRANE
                     AEIVLRSLEEGMRHRFFARGVLSPRDSDRGASIRGVPVLGGYDELERIVGEANDEGRP
                     IARLVFAPSEFTSSAESERLVVTARRLGIALSRLQTVEFGGGSALTPVEIEDLLFRRT
                     VETDRDRLARFVRGRRIVVTGGGGSIGSEISAAVAALGAAELLIVDNSEPALFAVGEA
                     LGAANVAARLRLTVADVRDRARVAALFDEFKPDLVFHAAALKQLPQLELDWTEGVKTN
                     VLGSANVVDAAIAVGAAAVLISTDKAVDPVSVLGATKRLSEMYGQLIDGGPAPAGAPR
                     IISVRFGNVLGSVGSVVPKFKAQIERGGPVTVTHPDMVRYFMTVREAVDLVISAAAHA
                     DSTVGGEARASVYVL"
BASE COUNT         1270 a         2681 c         2647 g         1350 t
ORIGIN      
        1 ggcgatcttt tcgcgcgcgt cgcgcagcgc tcctatcccg ccttgcgcgg cggcaccgag
       61 gagacgacct ccggccgcgc gcgcctggcg ccactgcgcg aggcgatcaa ggttccggtg
      121 ctggtcatca acgacagccc gatcaagcaa ttcgccgaga accgccacgc cgtcggccag
      181 agcctattcg agagctatct gcgcttcacc aatcgctcga ccaacggcaa gcgcgcgacc
      241 gtgtttggct acggcgcctg cgggcgcggc gtcgcggcct gcttccgcaa cgcgttcagc
      301 gcggtcagcg tcgtcgacgc caatccggtg acgacgctcg aagcccatct cgacggcttc
      361 gccacgccgc tgcgcgaggc ggcgatccgc ggcgccgatg tgctgatcac cgtgaccggc
      421 gccaaggacg tgttgaccgc cgccgatctg ccgctgatca agaacggcgc gatcttgatg
      481 aatggcggcc atctgccgca tgagatcgac gtcgccggcc tgcgcgcgca cgcctcggtg
      541 atcggcgccg acctttaccc gagcgaggga atcgaaacgc tgcgcctcgc cgacggccgg
      601 gcgatccaca ttctcggcgc cggccacatg gccaacctcg ccgggccgcg gccgctcggc
      661 aattcggtcg aatcgatgga tctcggcttt acgctgcaag cgcgctgcct cgagcgcgtc
      721 gcacgcggcg gactgggaca ggatcactgc gtcgtccccg ttctcgccga tatcgacgcg
      781 gccgtggcct cagcctatct ggcgatgaac cgctgacgcc ttctcccgaa gggctgtccg
      841 ggaaaatgag catgaaccga gtcgcttcag gcggcgatat cgcaaaacaa gaacgtttcg
      901 ggatttcctc gccgaatttc tgttgtcgag actcagaaaa gagcaggcgc agccctcaag
      961 gcttttcgaa ccttacaggc gcgcaatgac gcaaatccca aacgccgcgc tgccatcgac
     1021 gcggaggcgc gtcaaggtca agccgcttcg cggtcgctcc gcttcgcttc gcgaaccttg
     1081 acacgcctcc gcgtcgatgg ccgtttgtcg ccaagacgtt ttgctgctcg aaacggccga
     1141 atggccggtt tcgacagcaa aacgtcttgg tagccaataa gccacgccga ccgatcgcag
     1201 tcacccgaag ggccggaacg aagtggagga cccgccatag cgcctctccg ccgcgtgtcc
     1261 gttcatacgg gcgttgggag cgctatggcg ggttgacggc gatcgggcgg cgtggcgcca
     1321 cagcgctgga agttaagtcg ccattcagcg caaggcgcat cgctggcgat tttccccgga
     1381 cggccctgcc tctcccgaag ggatgggccg ggttgagggt tacgagcgca gagtaccttg
     1441 cgacgtactt ctcgttcgcg ggggaggatc gctacttctc tgtcagcttc agctcgatgc
     1501 ggcggttgcg cgccagcgac tcctcgtccg tgccttgatc gatcggctgg aattcgccga
     1561 agccggcggc cgccatgcgg tcgggcggca cgcccttggt aatcaggtat tgcacgaccg
     1621 cgatggcgcg ggcggcggac agctcccagt tcgacttgaa cgggccgccg gttatcggat
     1681 gcttgtcggt atggccgtcg acccgcagga tccacgggat ttcgggcggg atttcgcgcg
     1741 ccaattcgtt caccgcgacg gcgagatcgt cgagcgcctg gcggccggcg acgctgatat
     1801 cggccctgtt cgagtcgaag aggaccgagg attcgaaaac gaagcggtcg ccgaccacgc
     1861 gcacgcccgg gcgcgagccg agaatttgcc gcaagcggcc gaagaaatcg gagcggtagc
     1921 gggcgagctc ctgcaccttc tgcgccaggg cgacgttcag ccgcgagccg agatcggcga
     1981 tcttggcctg cgattcgcgc tgcgaggcct cggaggcggc cagagcgtcc tcgatcgcgg
     2041 cgagctgccg gcgcaaggcg gcgatctgct ggttgagaat gtcgacctgg gcgagcgccc
     2101 gcgtcgagac ctgcttctgc gcttcgagcg cattggccgc ggccgaggcc ttgtcgccgg
     2161 cgctgtcggc attggcgctg ttgagcgcga tctggttttg cagcttctgc tgctcctgct
     2221 gtgaagcttg cagcgtcgcc gaaaggcccg cgagattctg ctcggcctcg ttcttggcgg
     2281 tgcgctccat agccagcagg ctggtcagct cctcgatctg gtgattgagc ttgctcagca
     2341 ccgtgtcctt gccgctcacc tcgcgcgaca ggaagaactg ggcgagcatg aagaccgaca
     2401 gaaggaagat gatgacgagc agcatcgtcg acaaagcgtc gacgaagccc ggccagtagt
     2461 tgacgccttc cgaacggcgg ccgcgcgcca gcgccatcat acgctcccga acaatggacg
     2521 gtcacatccc atgcgtctcg ctctcgccgg caaggcggcg cagcagcttg ccgacctctt
     2581 cctgctgggc ggcctgggct tcgacccagt cgcggataag ctgctgctcc tggcgcatgt
     2641 gattgacgag gccctggatg ccttcggcga gattggccat ggcggcggcg ctcttgcgct
     2701 gctcggccgg gtcgtaggcg accggcagct tgtccgggat catttcgacg ggcggcggcg
     2761 gcgcgctggt ctcgacatgg ctggcgagga aatcttcgag ctcggtatag aaccggttct
     2821 gcgcctggcc ggcctgcagg tcgagaaagc cgaggatgag cgaaccggcc aagccgaaca
     2881 gcgatgaggt gaaggaaatg ctcatgccgg cgatcggctt ggaaaggccg cttttcagat
     2941 cgtcgaacat cgcgccggcg tcgacattgg tctgcatcgc gttgatcacg ccgccgatcg
     3001 agcgcaccgt ttcgagcaag ccccagaacg tgccgagcag gccgagaaag accagaaggc
     3061 cgatcaggta gcggttgagc tcgcgcccct cgtcgagccg ggttccgacc gagtcgagga
     3121 tcgcgcggga ggtggtggtg gagagcccgc gcgcgccgac gccggcattg agcagcgtcg
     3181 ccatcggccc gagcaggcgc ggccccttgt tcgagcgcca cggccgagcg ccgctcagcg
     3241 aattgaccca accgatttcg cggaacagcc ggatgacctg gcgaaaggtg aaaaacgacc
     3301 cgagcagcag gacgccgatg atgaagccgt tcagcccagg attggcgtga aaagccgtct
     3361 cgatctgctt ttcgaggatc aggacgacga aagcgacgat gatcaggaac gccgccatcc
     3421 cgatcaggaa tatgcggggc gaggtgaggg gccggacttc ggattcgatc gccatgcgtt
     3481 gtgggccgtt tcgcatcaac atgcgcgccg caccctatca gaaatcgcgc ccgcggcgcc
     3541 acgcgccgaa agcggaaggc cgccggccaa ggccgagcgg cgccgcggcg gcgagcgccg
     3601 gctttcgggg agttcctgcc atggagccgc tctatggccg cgatttgtcg ccgaaattgt
     3661 ggccgaagcg cttgcgagcg agagtttttg cttaggcgtc cttcagcaag gccagcattt
     3721 cgcgatgcat gatctcgttg ccggcgcaga tcgagccggt ttcgagaaaa ttctcctcgc
     3781 cgtcggcgcc ggtgacgaag ccgcccgcct cgcgaatcag cagcgcgccc gccgcgatgt
     3841 cccaggaatt gaggccgcgc tcccagaagg cgtcgaacag gccggccgcg acataggcga
     3901 ggtcgagcgc ggcgcaaccc atgcgccgga tgttgacgac cctcgccatg accgcggcga
     3961 gctcggcctt gaagcgcgga tgctgcgccg ccttgccgag atggggcacg ccgcagccga
     4021 tcagcgcctg cgacagttcg cggcggttgg cgacgcgcag gcgccggttg ccgccgaacg
     4081 cgccctggcc cttctcggtc acgtacatgt cgtcggtggc ggggttgtaa atcacgccgg
     4141 cgacgatctg gccctcccgc tccagcgcga tcgagaccgc gaacatcggg atggcgtgga
     4201 gaaaattggt cgtcgcgtcg agcggatcga tatgccaggt atgagtcttg tccgagcctt
     4261 cgacgcggcc gctttcctcc atcacgaagc cgtagcccgg acgcgccttg gacaattcct
     4321 cgaacaggag tttctcggcc tttttgtcgg cgaggctgac gaaatcgccc ggccccttca
     4381 tcgagacctg caaattgccg acctcgccga aatcccgctt caggccgcgg ccggctttga
     4441 tcgccgcgct cgtcatcaca ttcatcaaag cggagcggat catcgcgtcg tcccgtcggc
     4501 gttccggccg cggccgcgac ccttgaaagg gctcatctgc ccgccgcgtc cggcaaggtc
     4561 aagcgcggcg gcggttgcgc gtcgccggcc gccataggat ggtggcccga tgaaaagcga
     4621 catcgaaatc gctcaagccg ccaagctcga agcgatcgcg gccgtcgccg ccaagctcgg
     4681 cgtcccggag gcggcgcttc atccctatgg ccggcacgtc gccaagatcg atttcgattt
     4741 tctcgattct ctcgccgagc ggcccgacgg caagctgatc ctggtcaccg cgatcaatcc
     4801 gacgccggcc ggcgaaggca agaccacgac gacgatcggc cttggcgacg cgctgaacct
     4861 tatcggcaag cgcgcggcga tctgcctgcg cgagccgtcg atgggcccgg tcttcggcca
     4921 gaagggcggc gcgaccggcg gcggatgggc gcaggtcgcg ccgatggtcg acatcaacct
     4981 gcatttcacc ggcgacttcc acgcgatcgc cgccgccaac aatctcctcg cggcgatgat
     5041 cgacaatcac attcattggg gcaatgagct cggcgtcgat ccgcgccgca tcctcttgcg
     5101 gcgcgccctc gatgtgaacg accgcgcgct gcgccagacg atcgccggcc tcggcggccc
     5161 ggccaacggc tatccgcgcg aggacgggtt cgacatcgtc gtcgcctcgg aggcgatggc
     5221 ggtcttctgc ctggcgacca atctcgacga cctgcaaaag cggctcggcc ggatggtcgt
     5281 cgcctatacg cgggagaaaa agccggtcac cgccgacgat ctcaaggcga ccggggcgat
     5341 ggcggttttg ctcaaagacg cgctgcggcc gaacctggtg cagactttgg aaggctcgcc
     5401 ggctttcgtt cacggcgggc ctttcgccaa tatcgcccat ggctgcaact cggtgatcgc
     5461 caccaaggcg gcgctcaagc tcgccgacta cgtggcgacc gaggccggct tcggcgccga
     5521 tctcggggcc gagaaattct tcgacatcaa atgccggcag gcgggcttga agccggccgc
     5581 ggcggtgatc gtcgcgagcg tgcgcgccct gaaaatgaat ggcggcgtcg gccgcggcgc
     5641 gctggcggcg gagaatctcg ccgcgctcga aaccgggctc gccaatctcg gcggccatgt
     5701 cgccaatatg cgcaaattcg gcgtgccgat cgccgtcgca atcaatcatt ttgcaaccga
     5761 cacggaagcc gagctcgctc tgctccaaca agccgcgcgg gagcggttcg gcgccgaggc
     5821 gatcgtctgc cgtcactggg ccgagggcgg caagggcgcc gaggcgctcg cccgccatgt
     5881 cgtcgcgctc gccgatagcg gcgccgccgc cttcaagccg ctctatccgg acgacctgcc
     5941 gctctgggac aagatgaagc ggatcgccgt tgagatttac ggcgcgagcg acatttcggc
     6001 gagcgcggcg gcgcggcggc aattcgccga tctcgaagcc gccggctatg gccgcctgcc
     6061 gatttgcgtc gccaagaccc cctactcgtt ctcggccgat ccaagcctgc ttggcgcgcc
     6121 gagcggccac gttccgccga tccgcgaatt gcgcttgcgc gccggcgccg gctttatcgt
     6181 ggcgatcgtc ggcgacgtca tgaccatgcc cggcctgccg cgccggcccg gcgcggaaaa
     6241 gatcggcctc gccggcggcg tggtccaggg cctctcctga cctcccgggc cgcgagacgc
     6301 ccgcctcgcc tccgccttgc gaattggcta tagatcgtcc gcgccgagcc aggggcgctc
     6361 gcttgggtgc gatgaacgtg cattttctgt tgaatcgcgc caagcgcttc gcggtcatgg
     6421 cgcatgacct cgcgaccacg ccgctggcga tcgccctcgc cctctatttg agcttcccgg
     6481 ccaacgcccg tctccatacc gaggcgctgg cctggatcgt tccgctgttc acgctctatg
     6541 ccggcggggt cttccgcttc ttccgcctct acgaagcgaa atggcgcttc gcctcgctgc
     6601 ccgatctctt caacattttc cgcgccgcga gcacgttggc gatctcgctg ctcgtcgtcg
     6661 atctcgccat cgtggcgcgc ggcgtcgccg acggcttcgt cttcggcgag aaggtctgct
     6721 tcctctactg ggtcttgcag ctcatgctgc tcggcggccc gcgcatcgcc tatcgctact
     6781 ggcgctatgt ccaggcgcgg cggaccggac ggcgcgagcg cgcgccgtcg atcctggtgc
     6841 tcggccgggc caacgaggcg gagatcgtgc tgcgctcgct tgaagaaggc atgcgccacc
     6901 ggttcttcgc ccgcggcgtg ctgtcgccgc gcgacagcga ccgcggggcc agcattcgcg
     6961 gcgtgccggt gctcggcggc tatgacgaac tcgaacggat cgtcggcgaa gccaacgacg
     7021 agggccggcc gatcgcccgg ctggtcttcg cgccgagcga gttcacatcg agcgccgaat
     7081 ccgagcgcct cgtcgtcacc gcgcgacggc tcggcatcgc cttgtcgcgc ctgcagacgg
     7141 tggagttcgg cggcggctcg gcgctgacgc cggtcgagat cgaggacctc ttgttccgcc
     7201 gcacagtcga gacggatcgt gaccggctgg cgcgcttcgt gcgcggaagg cgcatcgtcg
     7261 tcaccggcgg cggcggctcg atcggctcgg aaatctcagc cgcggtcgcg gcgctcggcg
     7321 cggccgagct tctgatcgtc gacaattccg agccggcttt gttcgccgtc ggcgaggcgc
     7381 tcggcgcggc gaatgtcgca gcccggctcc gcctcaccgt cgccgacgtg cgcgaccggg
     7441 cgcgcgtcgc cgccctgttt gacgagttca agcccgatct cgtcttccac gccgccgcgc
     7501 tcaagcaatt gccgcagctc gagctcgatt ggaccgaggg cgtcaagacc aacgtgctcg
     7561 gctcggccaa tgtcgtcgac gccgcaatcg ccgtcggcgc cgcggcggtg ctgatctcga
     7621 ccgacaaggc ggtcgatccc gtctcggtgc tcggcgcgac caagcgcctt tcggaaatgt
     7681 acgggcaact gatcgacggc ggcccggcgc cggccggcgc gccgcgcatc atctcggtgc
     7741 gcttcggcaa cgtgctcggc tcggtcgggt cggttgtgcc gaaattcaag gcgcagatcg
     7801 agcgcggcgg cccggtcacc gtcacccatc ccgacatggt gcggtatttc atgaccgtgc
     7861 gcgaggcggt cgacctggtc atttccgccg ccgcccatgc cgattcgacg gtcggcggcg
     7921 aggcgcgcgc ctcggtctac gtgctcaa
//
LOCUS       DATGJW010000212        13810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_869484, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000212 DATGJW010000000
VERSION     DATGJW010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 13810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13810
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_869484"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..159)
                     /locus_tag="VKU03_06725"
     CDS             complement(<1..159)
                     /locus_tag="VKU03_06725"
                     /EC_number="2.6.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015241153.1"
                     /note="catalyzes the transamination of D-amino acids and
                     their alpha-keto acids; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-amino acid aminotransferase"
                     /protein_id="HLJ70993.1"
                     /translation="MTRVAFVNGAYRRHAEAEVHIEDRGYQFGDGVYEVCEIRDGALI
                     DEVPHLARL"
     gene            complement(294..1661)
                     /locus_tag="VKU03_06730"
     CDS             complement(294..1661)
                     /locus_tag="VKU03_06730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLJ70994.1"
                     /translation="MATDILIVDDEDDIRELVAGILSDEGHGARLARNSDEALAAIEA
                     RRPQLILLDIWLQGSRLDGLQLLEIVKKQHPNVPVVMISGHGNIETAVTAIKLGAYDF
                     IEKPFKSDRLILIAERALETSRLKREVSDLRARAGSVDRLVGSSAAINNLRRVIERVG
                     PANSRVLISGPAGAGKELTARLIHAHSARAAGPFVVINAPTMAPERMEAELFGVEANG
                     ARARSVGALEEAHGGTLYFDEVADMPLETQSKILRVLVDQNFQRVGGAARVHVDVRII
                     SSTSRDLAEAITEGRFREDLFHRLSVVPIRAPSLSERREDIPELIEFFMDQISAAMGA
                     PRRRIAPDAMAVLQSHDWPGNIRQLRNNVERLMILAAGESDSEITAALLPSEIGALVP
                     SIPSGVGGEKLMSLPLREAREVFEREYLVAQINRFGGNISRTAEFIGMERSALHRKLK
                     SLGVD"
     gene            complement(1661..3919)
                     /locus_tag="VKU03_06735"
     CDS             complement(1661..3919)
                     /locus_tag="VKU03_06735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing sensor histidine kinase"
                     /protein_id="HLJ70995.1"
                     /translation="MTRRAKGEGAVARSDESRSVGRFGPAAVAAALALSIFTFVVFAG
                     FTPVIPTRPVVLAIFSGNAVLIVILLALIALETRKVISARRAGRAGARLHSRVVALFS
                     LVAAVPALVTAVVATVSLERGLNPVFMEDIRSFIGKTTEATHLYRESQCRSLLRDAEL
                     TASDLSQSANLFQTDRSLFHDYLNSRAKFLNFSVAVIMKSNGAIEDRADESDPRQIAK
                     PAPADFQDAQKEAPLCLLLADGKTFIALRPISGIEDSYLYVGRPIDPFSESFSRDAAE
                     VVSTYDLFDAHRRNIEIGFGVMFILLALTMLLAAIWLGLAFANQLVAPIRRLIRAADE
                     VASGNLYVQVPTRRSEGDLGHLGETFNKMTQELSQQQNRLIAANSLNDERRAFTEAVL
                     AGVPAGVIGVDGNGVITVRNAAADRLLAPIAEPAGLIGVKLESLLPEVGPILQEAREG
                     RLRLHQGQAKLQRDGRDRVLNVRVTSNPQRPDQGVVVTLDDISDLITAQRTAAWADVA
                     RRIAHEIKNPLTPIQLSAERLKRRYGKLILEGRDVFDQCTETIIRQVDDIKRMVDEFS
                     SFARMPKALPARDDLVDCVRQVLFLMRVGRPEIALEDNLPAAPLFSKFDRRLMSQALT
                     NVIKNAGEGIDAIDEAARGQGRIVVDLQIDHERRAIISVSDNGKGFPAEDRHKLMEPY
                     VTTRAEGTGLGLPIVIKILEDHGGAVELLDGLERIDGGRGAQVRLILPIVEEAKTGES
                     EESRNNQRALLN"
     gene            complement(4038..5513)
                     /gene="ntrC"
                     /locus_tag="VKU03_06740"
     CDS             complement(4038..5513)
                     /gene="ntrC"
                     /locus_tag="VKU03_06740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrogen regulation protein NR(I)"
                     /protein_id="HLJ70996.1"
                     /translation="MPSGHILVADDDAAIRTVLNQALSRAGYEVRSTGTVAGLWRWVA
                     AGEGDLVITDVVMPDDNAFELLPRIKRLRPDLPIIVMSAQNTFMTAIKASERGAYEYL
                     PKPFDLKELVGIVGRALSEPKRRKGAEIEPTPAESMPLVGRSPAMQDIYRSLARLMQT
                     DLTVMITGESGTGKELVARALHDYGKRKGGPFVAINMAAIPRDLIESELFGHEKGAFT
                     GASQRSIGRFEQAEGGTLFLDEIGDMPMEAQTRLLRVLQQGEYTTVGGRTPIKTDVRI
                     VAATNKELRYAIQQGSFREDLFFRLNVVPLRLPPLRERVEDIPDLIRHFFAQGLAEGL
                     PAKHIEPAALDRLKRYPWPGNIRELENLVRRLAALHPQETISEALIGTELGLNAPLAT
                     PTLAGPAPGGRSAAAATGESFAADMERSVAELFRSFGAEPPPVGLYHRVLRELEAPLI
                     AATLAATRGNQIKAAEILGLNRNTLRKKIRDLDLKSIRAIG"
     gene            complement(5569..6681)
                     /locus_tag="VKU03_06745"
     CDS             complement(5569..6681)
                     /locus_tag="VKU03_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ70997.1"
                     /translation="MNAPVEPDAFSDAMRLLNALPQPVLAIDSAGRVREVNSAAENFF
                     DMGRAALQRSRLADLVPFGSPVLQLVEETMSAEAPINGYKLDISSPRLGAGRLVDAFV
                     APLGGGAVLMLQERSIAEKMDRQLIHRGAARSVTALGAMLAHEIKNPLSGIRGAAQLL
                     DSAVSDEDRALTRLICDETDRIVKLVDRMESFSDEPPGARESLNIHAVLDRVRMVATA
                     GFARHIRFELAYDPSLPPIRGSRDQLIQVFLNLVKNAAEAIDQTGADGEIVLSTAYRP
                     GVRLKTQAAADPVSLPLEVCVRDNGPGVPPDIASHLFDPFVTTKASGTGLGLALVAKV
                     IGDHGGVVEFESLPRRTTFRVLLPLDAQTGRATGAP"
     gene            complement(6678..7697)
                     /gene="dusB"
                     /locus_tag="VKU03_06750"
     CDS             complement(6678..7697)
                     /gene="dusB"
                     /locus_tag="VKU03_06750"
                     /EC_number="1.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0017150 - tRNA dihydrouridine synthase
                     activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA dihydrouridine synthase DusB"
                     /protein_id="HLJ70998.1"
                     /translation="MDVAQNLCSNREPPLRIGATILNGRALLAPMSGVSDVGMRRAAL
                     RFGAAAAATEMVACNSYLADESETVLRAEGEGIRPHVMQLVGCEANSMALAAKRAEDA
                     GAAVVDINMGCPARRVAGALAGSALMRDLDQAQRLIEAVVAAVRTSVTLKMRLGWDDS
                     SRNAPELARRAEAAGIVMATVHGRTRNQFYNGRADWAAVRAVVESVSIPVVVNGDCAG
                     LDDAEEMLRLSGAAAVMVGRAAIGAPWLVGQIAAGLESGRPAAAPSAALRREAALEHF
                     ETLLLLFGAHAGLRHARKHLSAYAEKAGAPAESRLALVTTEDVSQARALLGEAFNYAE
                     EREAA"
     gene            7797..8996
                     /locus_tag="VKU03_06755"
     CDS             7797..8996
                     /locus_tag="VKU03_06755"
                     /EC_number="2.7.7.60"
                     /EC_number="4.6.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008685 - 2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase activity [Evidence IEA];
                     GO_function: GO:0050518 - 2-C-methyl-D-erythritol
                     4-phosphate cytidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0016114 - terpenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-C-methyl-D-erythritol 4-phosphate
                     cytidylyltransferase/2-C-methyl-D-erythritol
                     2,4-cyclodiphosphate synthase"
                     /protein_id="HLJ70999.1"
                     /translation="MPTSPKVSLIVLAAGRGTRLGGDEPKQYRLCAGRPLLCHTLESL
                     AAAYDFAATTVVIHPNDRPLYDRALSFLSPACAATLGPPALGGATRQESGLAGLAAQE
                     PLQPDLVLIHDGARAFPPADLILRAVAAAAEHGAAAPGLPLSDTIKEIDQAGIVVATP
                     IRANLRAMQTPQAFRFDLIYDAHRRAARERREDFTDDAAIAEWAGWPTHIFPGAAANV
                     KITEMSDLVAAEARLMTQASDIRIGQGFDVHAFAEGDGVWLGGVHIPHDRALAGHSDA
                     DVALHAITDALLGAIGDGDIGAHFPPSDEKWRGAPSRIFLEDAARRVRERGGLIANID
                     ATIICERPKIGPHRDAMRARIAEIAGLAPDRVAVKATTSERLGFTGRGEGIACLAVAT
                     VRLRPAA"
     gene            9020..9514
                     /locus_tag="VKU03_06760"
     CDS             9020..9514
                     /locus_tag="VKU03_06760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CinA family protein"
                     /protein_id="HLJ71000.1"
                     /translation="MFPDHLQRRAAALIGLCRGKGLKIACAESCTGGLLSGLLTEIAG
                     SSAAFERGFVVYSNEAKQDVLGVSAETLRDYGAVSAEAARAMAIGALAHSRADLAVSI
                     TGIAGPGGGTPQKPVGLVHFACAKTGGFVETAEGRYGDIGRAAIRLTAVEAALGLLEA
                     AARS"
     gene            complement(9511..9978)
                     /locus_tag="VKU03_06765"
     CDS             complement(9511..9978)
                     /locus_tag="VKU03_06765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011314709.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RatA family
                     toxin"
                     /protein_id="HLJ71001.1"
                     /translation="MPSFRTTRRVRHSAAEMFDLVADVERYPSFVPLCKSLTVKQRIR
                     RAGGAEVVLAEMEVGYRAIKERFTSRVTLDRPRLKVLVEYVDGPFSHMENVWAFHDEP
                     AQPQPCSRIEFFISYQFRSRMLGALMGSMFDAAFRRFAAAFEAQADAVYGAPP"
     gene            complement(10030..10266)
                     /locus_tag="VKU03_06770"
     CDS             complement(10030..10266)
                     /locus_tag="VKU03_06770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1467 family protein"
                     /protein_id="HLJ71002.1"
                     /translation="MSTPMAVAVFFTTWWIVLFAVLPFGVRSQHEAGTAEALPAGADP
                     GAPVAPMLVRKALWTTAISAVLFAALYAYMTLNP"
     gene            complement(10294..10719)
                     /gene="secG"
                     /locus_tag="VKU03_06775"
     CDS             complement(10294..10719)
                     /gene="secG"
                     /locus_tag="VKU03_06775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015932016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HLJ71003.1"
                     /translation="MQGFLIVVHLLVVIALVTVVLLQRSEGGALGIGGGGGFMTGRGQ
                     ANALSRATAILATLFFATSLIMSILAGWSRSPRSILDSATPPPATSQPANPASPAKAN
                     NGGNLLDQLKKMENQKNEAPAAPAPAQPAPAAPADQTKP"
     gene            10894..11688
                     /locus_tag="VKU03_06780"
     CDS             10894..11688
                     /locus_tag="VKU03_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="slipin family protein"
                     /protein_id="HLJ71004.1"
                     /translation="MVAPVLQWIAVHPVETAVIVLIVLLFLRSIRVANQYQRAVVFFL
                     GAYNRTAGPGVYILLPFIEWQVMQDLRVVTHAMEQQETITRDNVPIKINAVIWRRIVN
                     PAKAVIEVRDVGDAVLQIAVTALRKEIGRHSLDDVLREQDQISAALQKAMDEATEPWG
                     VKVERVEMKNIEIPPTMQRAMAQEAEALREKRARIIKAEAELSAAEKLRDAASVVMQI
                     PAALELRRMQMITEVGAEQNTMTIVMMPSEFVELARSLSNAAAKKG"
     gene            11746..12420
                     /locus_tag="VKU03_06785"
     CDS             11746..12420
                     /locus_tag="VKU03_06785"
                     /EC_number="3.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD family hydrolase"
                     /protein_id="HLJ71005.1"
                     /translation="MRIEPVFLFDLDGTLVDSVYQHVLAWKEALDAEGIELSVWRIHR
                     KVGMSGGLFMNQLLRETGAAISPERVDSLRLAHAAAYRRYAEQIRPLPGARELLNWLT
                     EARIPWAIATSGRMETAAVNLAALGVDPNRVPVVTRDQVKYAKPDPDLFLAAAERLNA
                     PIEQAFVIGDSIWDMLAAKRCRALGVGLLSGGYGLDELQQSGAFRVYEDPADMLRHID
                     EVGGRR"
     gene            12460..13184
                     /locus_tag="VKU03_06790"
                     /pseudo
     CDS             12460..13184
                     /locus_tag="VKU03_06790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007691799.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase"
     gene            complement(13245..>13810)
                     /locus_tag="VKU03_06795"
     CDS             complement(13245..>13810)
                     /locus_tag="VKU03_06795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLJ71006.1"
                     /translation="DNLDHGGRAFLAQARTTFTTPAGASRLGGNAVGLEAWQSLKFQS
                     EKGAVHVAGAPARHGPPGIEPISGEVAGFLIGLAEPGDALYFAGDTVWYEGVAEVARR
                     YAPKLVILNTGSAEPRGKFHVTMDANDAIEAAHAFPQAKIAAIHNEGWRHFAESADDL
                     AQAFRTLGLADRLLPLERGRTVKLTLA"
BASE COUNT         2380 a         4581 c         4540 g         2309 t
ORIGIN      
        1 gagccgcgcc agatgcggga cttcgtcgat cagcgcgccg tcgcggattt cgcagacttc
       61 gtagacgccg tcgccgaatt gatagccgcg gtcctcgata tggacctcgg cctcggcgtg
      121 acggcgatag gcgccgttga cgaaagcgac gcgggtcatc ggtcccttaa aaatctgctt
      181 gcatcattcc cgcgaaagcg ggaatccagg aagcctccgc gcttgccggg cgggccatgc
      241 cggattcccg attgcgcagg aatggcgctg ggtgaagtac gaaagccgtc cactcaatcc
      301 acgccgagcg atttcagctt gcggtgcagc gcggagcgct ccataccgat gaattcggcg
      361 gtgcgcgaga tattgccgcc gaaccggttg atctgcgcga cgagatattc gcgctcgaag
      421 acttcgcggg cttcgcgcaa aggcaggctc atcaatttct caccgccgac gccgctcgga
      481 atcgacggca ccaaagcgcc gatttcggac ggcaacaaag ccgcggtgat ttccgaatcg
      541 gattcgccgg ccgcgagaat catcagcctt tcgacattgt tgcgcaattg gcgaatgttg
      601 ccgggccaat catgcgattg cagcaccgcc atcgcgtcgg gcgcgatgcg gcggcgcggc
      661 gcgcccatcg ccgccgagat ttgatccatg aagaattcga tcaactccgg aatgtcctcg
      721 cgccgttcgg acagcgacgg cgcgcggatc ggcacgaccg acagacgatg gaacagatct
      781 tcgcggaagc gcccctcggt gatcgcttcg gccagatcgc ggctggtcga tgaaatgatc
      841 cgtacgtcga catggacgcg cgccgccccg ccgacgcgct ggaaattctg atcgaccagg
      901 acgcgcagga ttttcgattg ggtctccagc ggcatatcgg cgacttcgtc gaaatacagc
      961 gttccgccat gagcttcttc cagcgcgccg acggagcgcg cccgcgcgcc attggcctcg
     1021 acgccgaaaa gctccgcctc catccgctcc ggcgccatgg tcggggcgtt gataacgacg
     1081 aacggcccgg cggcgcgcgc cgaatgggcg tgaatgagcc gcgccgtcag ctccttgccc
     1141 gccccggccg gtccggaaat caggacgcgc gaattggccg gtccgacgcg ctcgatcacg
     1201 cgccgcaaat tgttgatcgc cgccgacgag ccgaccaggc ggtcgaccga accggcgcgc
     1261 gcgcgaagat cgctgacttc ccgcttgagg cgcgacgttt cgagcgcgcg ctcggcgatc
     1321 aggatcagcc gatccgactt gaacggcttc tcgatgaagt cataagcgcc gagcttgatc
     1381 gcggtgaccg ccgtttcgat gttgccatga ccggaaatca tcaccaccgg cacattcgga
     1441 tgctgcttct tgacgatttc gagcaattgc aagccgtcga gccgcgagcc ttgcaaccag
     1501 atgtcgagca gaatgagctg cgggcggcgc gcctcgatcg cggcgagcgc ctcgtcggaa
     1561 ttgcgcgcga gccgcgcgcc atgcccttcg tcggaaagga tgcccgcgac aagctcgcgg
     1621 atatcgtctt cgtcgtcgac aatgagaata tcggtcgcca ttagttcaac aaagctcgct
     1681 gattatttct cgactcctcg gactcgccgg tcttcgcctc ctcgacgatc ggcaggatca
     1741 ggcgcacttg ggcgcctcgg ccgccgtcga tccgctccag gccgtcgagc aattcgaccg
     1801 ccccgccatg gtcctcaagt attttgatga cgatcggaag gccgaggccg gtgccttccg
     1861 cgcgggtggt gacgtaaggc tccatcagct tgtggcggtc ttcggcggga aatcccttgc
     1921 cgttatcgct caccgagatg atcgcgcgcc gctcgtgatc gatctgcaag tcgacgacga
     1981 tgcgcccctg cccgcgcgcc gcctcgtcga tcgcgtcgat cccctcgccg gcgttcttga
     2041 tgacgttggt cagcgcctgc gacatcagcc ggcggtcgaa cttcgagaag agcggcgccg
     2101 ccggcagatt gtcctccagc gcgatttcgg gccggccgac gcgcatcagg aagaggacct
     2161 gccggacgca atcgacgagg tcgtcgcggg ccggcagcgc cttcggcatg cgcgcgaagg
     2221 aggaaaattc gtcgaccatg cgcttgatat cgtcgacctg ccggatgatc gtctcggtgc
     2281 attgatcgaa gacgtcgcgg ccttcgagga tcagcttgcc gtagcgccgc ttcagccgct
     2341 ccgcggaaag ctggatcggc gtcaggggat tcttgatttc gtgagcaatg cgccgcgcga
     2401 catcggccca ggccgccgta cgttgcgcgg tgatcaaatc gctgatgtcg tcaagcgtca
     2461 ccacgacgcc ctgatcggga cgctgcggat tgctggtcac gcgaacgttg aggacccggt
     2521 cgcggccgtc gcgttgcagc ttggcctggc cttgatgcag gcgcagccgg ccttcccgcg
     2581 cctcttggag gatgggaccg acctccggca agagactttc gagcttcacg ccgatcaggc
     2641 cggccggctc ggcgatcggc gccaggaggc gatcggcggc ggcgttgcgc accgtgatca
     2701 cgccattgcc gtcgacgccg atcacgccgg cgggaacgcc cgcgagcacc gcctcggtga
     2761 aagcgcgccg ctcgtcgttc agcgaatttg cggcaatgag ccggttctgc tgctggctca
     2821 gctcctgggt catcttgttg aaggtttcgc cgagatggcc gagatcgcct tccgagcggc
     2881 gcgtcggcac ctggacatag agattgccgg aggcgacttc gtcggcggcg cggatcagac
     2941 ggcggatcgg cgcgacaagc tggttggcga aagcaaggcc aagccagatc gccgccagca
     3001 gcatggtcag cgccagcagg atgaacatca cgccgaagcc gatttcgatg ttgcggcgat
     3061 gcgcgtcgaa gagatcgtag gtcgagacga cttcggccgc gtcgcgcgag aagctttccg
     3121 agaacggatc gatcggccgc ccgacataga gatagctgtc ctcaatgccg gagatcggac
     3181 gcagcgcgat gaaggttttg ccgtcggcga gcagcaggca gagcggcgct tctttctgcg
     3241 cgtcctgaaa atcggcgggc gcgggcttgg cgatttgtcg cggatcggac tcgtccgccc
     3301 gatcctcgat cgcgccgttc gatttcatga tcacagcgac ggagaaattg aggaacttgg
     3361 cgcgcgaatt gagatagtcg tggaacaggc tgcggtcggt ctgaaagaga ttggcgcttt
     3421 gcgacaggtc ggacgcggtc aattcggcgt cgcgcagcag cgagcggcat tgcgattcgc
     3481 gatagagatg cgtcgcttcg gtcgtcttgc cgatgaagct gcgtatgtcc tccatgaaga
     3541 ccgggttgag gccgcgctcc agcgaaaccg tcgcgacgac cgcggtcacc agggcgggaa
     3601 cggcggcgac gagggaaaac agcgcgacga cgcggctgtg cagccgcgcg ccggcgcgcc
     3661 cggcccgccg cgccgaaatg actttccgcg tctccagcgc gatcagcgcc aggagaatga
     3721 caatgagcac agcgttgccg gagaagatcg ccagcacgac gggccgggtc ggaatgacgg
     3781 gcgtgaagcc ggcgaagacg acgaaggtga aaatggaaag cgcgagcgcc gcggcgacag
     3841 ccgccggccc gaaccggccg acggaacggc tttcatcgga acgcgcgacc gctccttcgc
     3901 ctttcgccct tcgcgtcatt ccttcgcagc aatgcgccgg attgccccgg cgcgcccctt
     3961 aatcgttgca gtcctgcaac gttgttgcaa gattgcgaca attcggcggc gaagccaagt
     4021 tttccaaagg cggccgatca gccgatcgcg cggatggatt tcaggtcgag atcgcggatt
     4081 ttcttgcgca gcgtgttgcg gttgaggccg aggatttcgg ccgccttgat ctggttgccc
     4141 cttgtcgccg ccaaagtcgc cgcgatcagc ggcgcttcga gctcgcgcag cacgcgatga
     4201 tagagcccga ccggcggcgg ctcggcgccg aaagagcgga agagctcggc gaccgagcgc
     4261 tccatatccg cggcgaagct ctcgcccgta gccgcggccg cgctacgccc gcccggcgcc
     4321 ggcccggcca aagtcggcgt ggcgagcggc gcgttgaggc cgagctcggt cccgatcagc
     4381 gcttcgctga tcgtctcttg cggatggagc gcggcgaggc gccggacgag attttcgagc
     4441 tcgcgaatat tgccgggcca ggggtagcgc ttgagccgat cgagcgcggc cggctcgata
     4501 tgcttggccg gcaggccctc ggcgaggcct tgcgcgaaga aatggcggat gaggtcggga
     4561 atgtcttcga cccgctcgcg caagggcggc aggcgaagcg gcacgacatt gagacggaag
     4621 aacaggtcct cgcggaacga gccctgctgg atggcgtagc gcagctcctt gttcgtcgcc
     4681 gcgacgatgc ggacatccgt cttgatcggc gtgcgtccgc cgacggtcgt atattcgcct
     4741 tgctgcagga cgcgcaggag ccgcgtctgc gcctccatcg gcatgtcgcc gatctcgtcg
     4801 agaaacagcg tcccgccctc ggcctgctcg aagcggccaa tcgagcgctg gctggcgccg
     4861 gtgaaggcgc ccttctcgtg gccgaacaat tcgctttcga tcaggtcgcg cgggatcgcc
     4921 gccatattga tggcgacgaa cggcccgcct ttgcgcttgc cgtaatcgtg cagggcgcgg
     4981 gcgacgagct ccttgccggt gccggattcg ccggtgatca tcacggtgag gtcggtctgc
     5041 attagccgag cgagagagcg gtaaatgtcc tgcatggccg gcgaacggcc gaccagcggc
     5101 atcgactcgg ccggcgtcgg ctcgatctcg gcgcccttgc gccgcttcgg ctcggaaagg
     5161 gcgcgcccga caatgccgac cagctccttc aggtcgaaag gcttcggcag atattcgtag
     5221 gcgccccgct cggacgcctt gatcgcggtc atgaacgtgt tctgcgcgct catcacgatg
     5281 atcggcagat ccggccgcag gcgcttgatc cgcggcagca gttcgaaagc attgtcgtcg
     5341 ggcatgacga catcggtgat gacgaggtcg ccctcgcccg ccgctaccca gcgccacagg
     5401 ccggcgacgg tgcctgtcga acgcacctcg tagccggcgc gcgacagcgc ctgattgaga
     5461 accgtgcgaa tggccgcgtc gtcgtcggcg accagaatat gcccagacgg cattccatca
     5521 ctccagcagg gcgatcgccc tcagcctcaa gcttctcctc cctgcggcct atggcgcgcc
     5581 tgtcgcccgg cccgtctgcg catccaaagg cagcagcacc cggaaggtcg ttcgccgcgg
     5641 cagggattcg aactcgacca ccccgccatg gtcgccaatc accttggcga ccagtgcaag
     5701 tccgaggcca gtgcccgagg ccttggtcgt tacgaatgga tcgaaaagat ggctggcgat
     5761 gtccggcggc acgccgggac cattgtcgcg cacgcagact tccagcggca ggctgaccgg
     5821 atcggccgcc gcttgagtct tgagacgaac ccccggccga taggcggtgg agaggacgat
     5881 ttccccgtcc gctccagttt gatcaatcgc ttccgcggcg ttcttgacca aattcaggaa
     5941 gacttggatc aactggtcgc gcgacccgcg aatcggcggc agcgacggat cgtaggccag
     6001 ttcgaagcga atatggcggg cgaagccggc cgtcgcgacc atccgcacgc gatcgagaac
     6061 cgcatggatg ttgaggcttt cgcgcgcgcc tggcggctcg tccgagaagg attccatccg
     6121 gtcgaccagc ttcacgatcc ggtcggtctc gtcgcagatc agccgcgtca gcgccctatc
     6181 ctcgtcggag acggcgctgt cgagcaattg cgccgcgcct ctgatgccgg aaagcgggtt
     6241 cttgatttcg tgggcgagca tggcgccgag cgccgtcacc gagcgcgccg cgccgcgatg
     6301 gatgagctgc ctgtccattt tttcagcaat tgaccgttcc tgcagcatca gcaccgcgcc
     6361 gccgccgagc ggcgcgacga aagcgtcgac cagacgtccg gcgccgagcc gcggcgagga
     6421 tatgtcgagc ttgtagccgt tgatcggcgc ctccgccgac atggtctcct cgacgagctg
     6481 caggaccggc gagccgaacg ggacgaggtc ggccaggcgg ctgcgctgca aggcggctcg
     6541 ccccatgtcg aagaaattct cagccgcgct gttcacctca cggacccggc cagcagaatc
     6601 aatcgccaga acgggttgcg gcaaagcgtt gagaagccgc atcgcgtcgc tgaacgcgtc
     6661 aggttcgaca ggggcgttca tgccgcttcg cgctcttccg cataattgaa ggcttcgccg
     6721 agaagcgcgc gcgcttggct cacgtcctcc gtggtcacca aggcgagacg gctttccgcc
     6781 ggcgcgccgg ccttctcggc ataggccgag aggtgcttgc gcgcatggcg caggccggca
     6841 tgcgcgccga aaaggaggag cagcgtctcg aaatgttcga gcgccgcctc gcgccggagc
     6901 gccgcgctcg gcgcggccgc cgggcggccg ctttcaagac cggcggcgat ttgcccgacc
     6961 agccaaggcg cgccgatcgc ggcgcggccg accatcaccg ccgccgcgcc ggacaatctc
     7021 aacatctcct cggcgtcgtc gaggccggcg caatcgccat tgacgacgac cggaatggag
     7081 acgctctcga ccaccgcccg gaccgccgcc caatcggcgc ggccgttata gaactggttg
     7141 cgggtccggc catgcacggt cgccatgacg atgccggccg cctcggcgcg gcgcgccagt
     7201 tccggcgcat tgcggctgga atcgtcccag ccgaggcgca ttttcagcgt caccgaagtc
     7261 ctcacggccg cgacgaccgc ctcgatgagg cgctgcgcct ggtcgagatc gcgcatcaag
     7321 gcgctgccgg cgagcgcgcc ggcgacgcgc cgcgccgggc agcccatatt gatgtcgacg
     7381 accgccgcgc ccgcatcttc ggcgcgcttg gccgccaagg ccatggaatt ggcctcacat
     7441 ccgacaagct gcatgacgtg aggccggatt ccttcgcctt ccgccctcag cactgtctcg
     7501 ctttcgtcgg cgagatagga attgcaggcg accatttcgg tcgcggcggc ggcggcgccg
     7561 aagcgcaagg cggcccggcg catgccgaca tcgctgacgc cggacatcgg cgccagcaag
     7621 gcgcgcccgt tgaggatcgt cgcgccgatc cgcagcggcg gctcccgatt gctgcataaa
     7681 ttttgtgcaa cgtccattgt gcttacattc taatcaaaag cgcggcgcgg acaagccgga
     7741 gagttgacat cgaggcggga gggacgcgag agaggccgcc tgaccgcgga gcgcccttgc
     7801 cgacctctcc caaagtctcg ctcatcgtcc tcgccgccgg ccgcggaacc cggctcggcg
     7861 gcgacgagcc gaagcaatac cggctttgcg ccggccggcc gctgctttgc catacgctgg
     7921 aatcgctcgc ggcggcctac gatttcgccg cgacgaccgt ggtcatccat ccgaacgacc
     7981 ggccgcttta cgaccgcgcc ttatcctttc tctcccccgc ttgcgccgcg acgctcggcc
     8041 cgccggctct gggcggcgcc acccgccagg aaagcggcct tgccggcctc gccgcgcagg
     8101 agccgctcca gcccgacctc gtcctgatcc atgacggggc gcgcgccttc ccgccggccg
     8161 acctgatcct gcgcgccgtt gcggccgccg ccgagcacgg cgcggcggcg cccggcctgc
     8221 cgctcagcga cacgatcaag gagatcgacc aggccggcat cgtcgtcgca acgccgatac
     8281 gggcgaattt gcgcgccatg cagacgccgc aagccttccg cttcgatctg atttacgacg
     8341 cgcatcgccg cgccgctcgc gagcggcgcg aggatttcac cgacgacgcc gcgattgccg
     8401 aatgggccgg ctggccgacc catattttcc ccggcgccgc cgccaatgtg aagatcaccg
     8461 aaatgagcga ccttgtcgcc gccgaagcgc ggctgatgac ccaggcgagc gatatccgca
     8521 tcggccaagg tttcgacgtc catgccttcg ccgagggcga cggcgtctgg ctcggcggcg
     8581 tccacatccc gcatgaccgc gcgctcgccg gccattccga cgccgatgtc gcgctgcacg
     8641 ccatcaccga cgcgctgctc ggcgcgatcg gcgacggcga tatcggcgcg catttcccgc
     8701 cctccgacga aaagtggcgc ggtgcgccgt cgcggatttt cctcgaagac gcggcgcggc
     8761 gggtgcgcga gcgcggcggc ctgatcgcca atatcgacgc cacgatcatt tgcgagcggc
     8821 ccaaaatcgg cccgcatcgc gacgcgatgc gggcgaggat cgccgagatc gccggccttg
     8881 cgcccgatcg ggtggcggtg aaagcgacga cctcggagcg cctcggcttc accggccgcg
     8941 gcgaagggat cgcatgcctc gccgtcgcga ccgtccgcct gcggccggcg gcttgaccgc
     9001 ggccggagtc aagatgccga tgtttcccga ccatcttcaa aggcgcgcag cggccctgat
     9061 cggcctctgc cgcggcaagg ggctgaaaat cgcctgcgcc gaatcctgca caggcggctt
     9121 gctgagcgga ctgttgacgg aaatcgccgg ctcctcggcg gcgttcgagc gcggcttcgt
     9181 cgtctattcg aacgaggcca agcaggacgt gctcggcgtc agcgccgaga ctctgcgcga
     9241 ttatggcgcg gtcagcgccg aggcggcgcg cgccatggcg atcggcgcgc tcgcccattc
     9301 gcgcgccgat cttgccgtgt cgatcaccgg aatcgccggc cctggcggcg gaacgccgca
     9361 aaagccggtc ggccttgtcc atttcgcctg cgccaaaacg ggcggcttcg tcgagaccgc
     9421 ggagggtcgc tacggcgata ttggtcgcgc ggcgattagg ctgaccgcgg tcgaggcggc
     9481 gctcggcctt ttggaggcgg ccgctcgctc ctaaggcggc gcgccataga ccgcgtcggc
     9541 ttgcgcctcg aacgccgcgg cgaaccggcg gaacgcggcg tcgaacatcg agcccatcaa
     9601 ggcgccgagc atccggctgc ggaattggta cgaaatgaag aactcgatgc gcgagcacgg
     9661 ctgaggctgc gccggctcgt catgaaaagc ccagacattc tccatatggc tgaacggccc
     9721 gtcgacatat tcgaccagca ctttcaatcg cggccgatcg agcgtgacgc ggctggtgaa
     9781 ccgttccttg atcgcccggt agccgacctc catttcggcc agcaccactt ccgcgccgcc
     9841 ggcgcggcgg atgcgctgtt tcacggttag ggatttgcag agcggaacga agctcggata
     9901 gcgctccacg tcggcgacga ggtcgaacat ttcggccgcc gaatggcgca cccggcgcgt
     9961 cgtcctgaac gaaggcatcg acttctcgtt ggcgaaacct ttagccgccg cttcgggcga
    10021 tccgcctctt cacggattga gcgtcatata ggcgtagagc gccgcaaata ggaccgctga
    10081 aatcgccgtc gtccacaacg ccttgcggac gagcatcggg gcgaccggcg cgccgggatc
    10141 ggcgcccgcc ggcagggcct cggcggtccc cgcctcatgt tgcgatcgca cgccgaaagg
    10201 gaggaccgcg aacaagacga tccaccacgt cgtgaagaaa accgcgaccg ccattggcgt
    10261 cgacatcgaa acgtctcccc ggcggcttcg cccttatggc ttggtttgat ccgccggcgc
    10321 ggcgggcgca ggctgagcgg gcgccggggc ggccggcgcc tcgttcttct gattttccat
    10381 cttcttgagc tggtcgagca ggttgccgcc attattggct ttggctgggc tggccggatt
    10441 ggccggctgg ctggtcgcag gcggcggcgt ggcgctgtcc aggatcgagc gcggcgagcg
    10501 gctccagccg gccagaatcg acatgatcag ggaagtggcg aagaacagcg tcgccaggat
    10561 ggcggtggcg cgcgacaggg cgttggcctg gccgcggccg gtcatgaagc cgccgccccc
    10621 gccaatgccg agagcgccgc cctcggagcg ctgcaacagc accaccgtga ccagcgcgat
    10681 gacgacgagg aggtgaacga cgatcagaaa accctgcatt gactttgtcc tggcgctttc
    10741 gcccgcggag cgacgcgctc cttaaaaatc gggccgctcc tagcatggcg caaggcgcca
    10801 agtccatttc tgcggccatc cgccgctggc cgtcattcga cggccggcga aaggcctata
    10861 tgatcttgca gtcgccgcgc gaggggcttg gccatggtcg cacctgtcct gcaatggatc
    10921 gccgttcatc cggtcgaaac cgcggtgatc gtcctgatcg tgctgctctt cctgcgctcg
    10981 atccgggtcg ccaaccaata tcagcgcgcg gtcgtgttct tcctcggcgc ctataaccgc
    11041 accgccggcc ccggcgtcta catcttgctg cccttcatcg aatggcaggt catgcaggat
    11101 ttgcgcgtcg tcactcacgc catggagcag caggagacga tcacccgcga caatgtgccg
    11161 atcaagatca atgcggtgat ctggcggcgc atcgtcaatc cggccaaggc ggtgatcgag
    11221 gtcagggacg tcggcgacgc ggttttgcag atcgcggtca ccgccttgcg caaggagatc
    11281 ggccgccact cgctcgacga tgtcctgcgc gagcaggacc agatttcggc ggcgctgcaa
    11341 aaggcgatgg acgaggcgac cgagccctgg ggcgtcaagg tcgaacgggt cgagatgaag
    11401 aacattgaaa tcccgccgac catgcagcgc gccatggcgc aggaggccga ggcgcttcgg
    11461 gagaagcgcg cccgcatcat caaggccgaa gccgagctca gcgccgccga aaaattgcgc
    11521 gacgccgcct cggtcgtcat gcagattccc gccgccttgg agctccggcg catgcagatg
    11581 atcaccgaag tcggcgccga gcaaaacacg atgacgatcg tcatgatgcc gagcgaattc
    11641 gtcgagttgg cgcgaagcct aagcaacgcc gcggcgaaga aaggatgagg cgcttccctt
    11701 ctcccagcaa agcggggaga agggatgaaa cagaaggtcc cgcccatgcg catcgaaccc
    11761 gttttcctct tcgatctcga cggcacgctg gtcgacagcg tttatcagca cgtccttgcc
    11821 tggaaggagg cgctcgacgc cgagggcatc gagctctccg tctggcgcat tcaccgcaag
    11881 gtcggcatga gcggcggcct gttcatgaac cagcttttgc gcgagacggg cgccgcgatc
    11941 agcccggagc gagtcgacag tttgcgcctc gcccacgccg ccgcctaccg ccgctacgcc
    12001 gagcaaatcc gccccctgcc cggcgcgcgc gaattgctga actggctcac cgaggcccgc
    12061 atcccctggg cgatcgcgac gagcggacgc atggagacgg cggctgtcaa tctcgccgcg
    12121 ctcggcgtcg atcccaatcg cgtcccggtc gtcacccgcg atcaggtcaa atacgccaag
    12181 ccggatcccg acctcttcct cgccgcggcg gagcggctca acgcgccgat cgagcaggct
    12241 ttcgtcatcg gcgacagcat ctgggacatg ctcgcggcca agcgctgccg cgcgctcggc
    12301 gtcgggctgc tcagcggcgg ctacggcctc gacgagctgc aacaatcggg cgcgttccgc
    12361 gtctatgaag acccggcgga catgctgcgc catatcgacg aagtcggcgg acggcggtga
    12421 agcgacgacg ctcaatgaca gcgtgaatag gaattccaca tgccgtacga actctactac
    12481 tggccgagca ttcaaggccg cggcgagtat gttcgcctgg cgctggagga ggcgggcgcg
    12541 gattacgtcg atgtcgcgcg gtcggggaaa gcgggcatgg cggcgatgat gaagatgatc
    12601 gacggcaaag gtcgccccta cgcgcctttc gcgccgcctt tcctcaagga cggagagacg
    12661 atcgtctcgc atgtcgccaa ctcctctatc tcgggcccaa gctcggcctc gcgccgaaag
    12721 ccgaggtcct gcgaggcttc gctcatggcc tgcaactgac gatcaccgat ttcctcgccg
    12781 agattcacga cacgcatcac ccgatctcga cggaacttta ttacgaagat caggtcgaag
    12841 aggcgaaggc gcgggccaaa gcctttctcg aatatcgcct gccgaaattc atcggctatt
    12901 tcgagcgggt cctcgaggcc aatccgcaag gcgacaagca tagcgtcggc gccggttgga
    12961 cctatgtcga tctttcgctg ttccagacga tcgagggtct cgcctacgcc tttccccgcg
    13021 cgatgaagac gtttgacgga aaatatccaa agctcgcggc gctgcgcgaa gccgtgcgcg
    13081 ctcggccgaa taccgcgcgc tatctcagct ccgatcggcg cattccattc aaccaatcgg
    13141 gactgttccg gcattatccc gaactggacc gcgccgcgcg ttagagtatc gaggcgcgtg
    13201 tcctggattc ccgttttcgc cgaatgacat cttccgacta aacgttaggc cagcgtcagc
    13261 ttgaccgtcc tcccccgctc caggggcagc agccggtcgg cgagaccgag cgtgcggaag
    13321 gcttgcgcga ggtcgtcggc gctttccgcg aaatggcgcc agccctcgtt gtggatggcg
    13381 gcgatcttgg cctgcggaaa ggcgtgcgcg gcctcgatcg cgtcgttggc gtccatcgtg
    13441 acgtgaaatt tgccgcgcgg ctcggcggag ccagtgttga gaatgaccag cttcggcgcg
    13501 tagcgccgcg cgacttcggc gacgccttcg taccagaccg tatcgccggc gaaataaagc
    13561 gcatcgcccg gctcggcgag cccgatgagg aatccggcga cctcgcctga aatcggctca
    13621 atccctggcg gcccatggcg ggccggcgcg ccggcgacat ggaccgcgcc cttctcgctt
    13681 tggaatttca ggctttgcca ggcttcaagg ccgacggcgt tgccgccgag ccggctcgcg
    13741 ccggccggcg tcgtaaaggt cgtgcgcgcc tgagcgagga aggcgcggcc gccatgatcg
    13801 agattgtcga
//
LOCUS       DATGJW010000213         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1177484, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000213 DATGJW010000000
VERSION     DATGJW010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177484"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..749
                     /locus_tag="VKU03_06800"
     CDS             <1..749
                     /locus_tag="VKU03_06800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLJ71007.1"
                     /translation="GATAAGQGLVSLGTSGVIFAVTDRFVSLPERTLHAFCHALPGRW
                     HAMAVMLSAASALSWIAGILKRQDDIAALINEAEAFAAKPENVANAPVFLPYLSGERT
                     PHNDPAASGLFAGLRAEHGADALAFAVMEGVAFAFADGLDVIEAAGARPKSTLIVGGG
                     ARSAFWAQMIADVTSLTIDLPAGAEAGAALGAARLAMLAAGDGDERTVCAAPEAQKRF
                     EPRAERAALHAPRLRRYGALYRAERALRGG"
     gene            complement(989..1282)
                     /gene="rpmB"
                     /locus_tag="VKU03_06805"
     CDS             complement(989..1282)
                     /gene="rpmB"
                     /locus_tag="VKU03_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HLJ71008.1"
                     /translation="MSRRCELTGKAVQTGNKVSHSNRKTRTRFLPNLCNVTLISDSLG
                     RSVRLRVAAASLRSVEHRGGLDNFLLKAADGELSAGARALKSEIKKKLAVAAG"
     gene            1478..2326
                     /locus_tag="VKU03_06810"
     CDS             1478..2326
                     /locus_tag="VKU03_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HLJ71009.1"
                     /translation="MASDDRLSQAYAHCESLCRAHDRDRWLAALFAPAAARPHLFALY
                     AFAYEASRLPEIVREPAAGEIRLQWWREAIGGARREEALGAPVAAALGATIETFRLPL
                     KAFDDLLAARTFDLYHDPMPDLAAYETYCGETSSLIFRLAGLILGHGERGGAEAAGHA
                     GIAYATAGLLRALPIASARGQVYLPLDTLARNGVSREDLIARRDTPQLRAALREMRAL
                     ARDHYEKALAVRWPEALGAAFLPLALVPLYLKRMERPDYRPFETPAEAPQWRRQWALW
                     RAARRY"
     gene            complement(2497..>2773)
                     /locus_tag="VKU03_06815"
     CDS             complement(2497..>2773)
                     /locus_tag="VKU03_06815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLJ71010.1"
                     /translation="FDTIKIDRSFVASIGADEVNAALVRAVVSLGRELGIAVVAEGIE
                     TEAQRETLASQGCRYAQGYLFGKPRPAREWLDGMSAAAPEAPMRRSA"
BASE COUNT          403 a          977 c          965 g          428 t
ORIGIN      
        1 tcggcgcgac cgccgcaggc caggggctcg tctcgctcgg cacgtccgga gtgattttcg
       61 cggtcaccga ccgcttcgtc agcctgcccg agcgcacctt gcacgccttt tgccatgcct
      121 tgccgggccg ctggcacgcg atggcggtga tgctgtcggc cgcctcggcc ctgagttgga
      181 tcgccggaat cctgaagcgt caggatgata tcgccgcgct gatcaacgaa gcggaggctt
      241 tcgccgccaa gcccgaaaac gtcgccaacg cgccggtctt cctgccctat ctttcgggcg
      301 agcgcacgcc gcataacgat cctgccgcga gcggcctgtt cgccggcttg cgcgccgagc
      361 acggcgccga cgcattggct ttcgcggtga tggagggcgt ggctttcgcc tttgcggatg
      421 ggctcgacgt gatcgaagcc gcaggcgcgc gaccgaaatc gacgctgatc gtcggcggcg
      481 gcgcgcgcag cgccttctgg gcgcaaatga tcgccgacgt gaccagcctg accatcgatc
      541 tgcccgccgg cgccgaagcc ggggccgcgc tcggcgcggc tcgcctcgcc atgctcgcgg
      601 ccggcgacgg cgacgagcgg acggtctgcg cggcgccgga agcgcaaaag cgtttcgagc
      661 cgcgcgccga gcgcgccgcg ctgcacgcgc cgaggctgag gaggtacggc gctttgtacc
      721 gagcggagag ggcgctgaga ggagggtagt cccttctccc cgccttgcgc tacggcatgc
      781 acgcatctcg acgcggggcc acatcggccc ggcatcgtca agcggccata gcccgaccga
      841 ccgttggacg aaccgaccaa gctcggcgca gcgggctatg gccgctgcgc cgcttcgctt
      901 cgccccttcg ggtgacgacg ccgggccgat gcgccgcgct tcaagatgtg tgcatgccgt
      961 agcgccttgc cgggagaagg gaagccgctc agcccgcagc gacagccagc ttcttcttga
     1021 tctcgctttt cagcgcgcgg gcgccggcgg aaagctcgcc atcggcggct ttcagcagga
     1081 aattgtccag cccgccgcga tgctcgaccg agcgcagcga ggcggcggcg acgcgcaggc
     1141 gcaccgagcg gccaagcgaa tcggaaatca gcgtgacgtt gcagagattg ggcaagaagc
     1201 gggtgcgagt cttgcgattg gaatggctga ccttgttgcc ggtctgaacg gccttgccgg
     1261 tcaattcgca acggcgtgac atagcgcttt gcttcccttg agcgaaatgg gattccctaa
     1321 gagggccaag gataaacgct tcgaagggcc gcccgaaagc gtcctgcggc cataacgtcc
     1381 cggcttcttg ggtcgcgggt ctatagggga aggggcgaga ggcgtcaagc aaggggcggg
     1441 cgctcgctcg cgcctcggag gccgattata agccgccatg gcctccgacg accgtttaag
     1501 ccaggcctac gcccattgcg agagcctgtg ccgcgcccat gaccgcgacc gctggctggc
     1561 cgcgctgttc gcgccggccg ccgcgcggcc gcatcttttc gcgctttacg ccttcgccta
     1621 cgaggcctcc cggctgcccg agatcgtgcg cgagccggcc gccggcgaaa tccgcctgca
     1681 gtggtggcgc gaggcgatcg gcggcgcgcg ccgcgaggag gcgctcggcg cgccggtcgc
     1741 cgccgcgctg ggcgcgacga tcgagacgtt ccggctgccg ctgaaagcct tcgacgacct
     1801 cctcgccgcc cggactttcg atctttatca cgatccgatg ccggaccttg cggcctacga
     1861 gacctattgc ggcgagactt cgtcgctgat cttccggctg gccgggctca tcctcggcca
     1921 tggcgagcgc ggcggcgccg aggccgccgg ccatgccggg atcgcctatg cgacggccgg
     1981 actcttgcgc gcgctgccga tcgccagcgc ccgcggccaa gtctatctgc cgctcgacac
     2041 gctggcgcgc aacggggtgt cgcgggagga cctgatcgcg cgtcgcgata cgcctcagct
     2101 ccgcgcggcc ttgcgggaga tgcgcgcgct cgcccgcgac cactacgaga aggcgctcgc
     2161 cgtgcgatgg ccggaagcgc tcggcgccgc cttcctgccg ctggcgctcg tcccgctcta
     2221 tctcaagcgg atggagcgcc ccgactatcg ccccttcgag acgcctgcgg aagcgccgca
     2281 atggcgccgg caatgggccc tgtggcgcgc cgcgcggcgg tactgaggcg cgacctctct
     2341 ccgtaggacg agaggcaggg gcaaggtact gtcaggaatc agcccggtcg caccggtgga
     2401 ggggcctcgc cgcccgaaga cgatctcaac cgccgaagcc ccctccgcca tgcttcgcat
     2461 ggcgcgagcg ccgccgagtc gcgcctggag cgccccttac gcgctccgcc gcatcggcgc
     2521 ttccggcgcg gcggcgctca tcccatcgag ccattcgcgc gccgggcgcg gcttgccgaa
     2581 gaggtagccc tgcgcatagc ggcagccttg cgaggcgagc gtctcgcgct gcgcctcggt
     2641 ctcgatcccc tcggcgacga ccgcaatgcc cagctcgcgg ccgagcgaga cgacggcgcg
     2701 gaccaaggcg gcgttgacct cgtcggcgcc gatcgaggcg acgaaggatc ggtcgatctt
     2761 gatcgtgtcg aag
//
LOCUS       DATGJW010000214         6952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1177760, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000214 DATGJW010000000
VERSION     DATGJW010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1177760"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VKU03_06820"
     CDS             <1..269
                     /locus_tag="VKU03_06820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HLJ71011.1"
                     /translation="GGHLTRQVFARELKVVAAAAGLRSSQISPHALRHAFASHLLQNG
                     ADLRVVQDLLGHADISTTQIYTHVLDERAKAMVRDLHPLSDEEG"
     gene            complement(405..1835)
                     /gene="otsA"
                     /locus_tag="VKU03_06825"
     CDS             complement(405..1835)
                     /gene="otsA"
                     /locus_tag="VKU03_06825"
                     /EC_number="2.4.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003825 - alpha,alpha-trehalose-phosphate
                     synthase (UDP-forming) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha,alpha-trehalose-phosphate synthase
                     (UDP-forming)"
                     /protein_id="HLJ71012.1"
                     /translation="MAPRLILVSNRVNVPVAGVPRQAGGLTVAVNAALKQREGVWFGW
                     SGKVSKEGETPAASVILRRRRTYITLDLSENDFQEYYNGFANRVLWPVLHYRVDLAEF
                     TSVDLGGYLRVNSRFADALSPFITPDDVIWVHDYHLLPFAKELRGRGHNNPIGFFLHI
                     PCPPPDIIEALPHHDETMGALSHYDLVGLQTDQDADNLSRYFAALGANQRGPGSFEFD
                     GRKVRVRAFPVAIATAVYARAARNSVKSTLVEELRNSLVGKRLILGVDRLDYSKGIKH
                     RIQAFGHFLEKYPEWLNRVTFLQVTPKSRTGVPEYSAMEQEVSALVGQINGRFGDPAW
                     TPIRYVNRSYSRAMLAGLYRSAHVGLVTPLRDGMNLVAKEYIAAQDADDPGVLVLSQF
                     AGAAQELTGALVVNPHETEGMAAAIKAALEMPLDERKARFQRMYDHLVENDIDRWAER
                     FLSALGETREQPGLLSSLRQLFAVRA"
     gene            complement(1820..3664)
                     /locus_tag="VKU03_06830"
     CDS             complement(1820..3664)
                     /locus_tag="VKU03_06830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013215390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HLJ71013.1"
                     /translation="MARKVGRMSETDAKSRRRPGKQALDLALIGNCRTAALVDPVGRI
                     VWWCFPRFDSNPIFCRLLAGDEEKGFCDVVLNDLVDARSAYVRNTAILETELTDKQGG
                     RLRITDFAPRFQRYERTFHPPQLVRRITPLAGQPRITIRVRPTEAYGRPMSDLSIGSN
                     HIRYQGAGTVIRLNTDAPLSYVAQETTFPLTRPMTLVLGPDEPFRSAFEATANEFEEL
                     TRNYWLHWTRTLAIPYEWQNEVMRAAITLKMCSFEETGGIVAAHTTSIPEAANSGRNW
                     DYRYCWLRDAYFVTQALNKLGATDTMEAYLNYIMTIVTETDAPLRPVHSVVPGESLFE
                     EIAPNLAGFLGQGPVRVGNQAAEQHQHDVYGSAILAATQMFIDERLPRMGDESLYRRL
                     EPLGERAMRLALKPDAGIWEYRGRERIHTHSVSICWAACDRLAQIGRRLGNHERALLW
                     AERAASLRQEIFTRAWREDKKAFVGAFEGDELDASVLLLAELGLIAADDPRFVSTCEA
                     IGRDLMRSGRIMRYTARDDFGWPETAFLVCNFWYIDALAEIGRRDEARELFSDLLERR
                     NAFGIFSEDIHPETGVLWGNIPQTYSMAAIIDSAMRLSIKWEAAWRPV"
     gene            complement(3640..4380)
                     /gene="otsB"
                     /locus_tag="VKU03_06835"
     CDS             complement(3640..4380)
                     /gene="otsB"
                     /locus_tag="VKU03_06835"
                     /EC_number="3.1.3.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004805 - trehalose-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trehalose-phosphatase"
                     /protein_id="HLJ71014.1"
                     /translation="MTAPEPATLDRHSIGLFLDVDGTIIDLAPTPDAVDVPAALLREL
                     ASAERRLDGALALVSGRPIEELDRLFKPLRLRASGVHGGEIRYAPNEPGKSLATGRLP
                     ENAWMRLKRIVARFPGAFAENKGLSFAVHYNLAGPVAEDICKALQQFLHEYPELPLEV
                     IGGRSVYEIKLAGFDKGKAIECFMARPPFAGRRPIFVADDRVDRPGFETAVALGGLAL
                     SVGAELPGVSGWFASPKDLRAWLERLGG"
     gene            4641..5939
                     /locus_tag="VKU03_06840"
     CDS             4641..5939
                     /locus_tag="VKU03_06840"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ71015.1"
                     /translation="MRAETRQGREDCVAADRLQPSAAAARQRPDAVGLQRLLFFLLPA
                     AFALYANFQGVQLILIPAQVETVEPARKIDNLALLTVFCAIGGVIGLMAGGGVSDATR
                     SRWGRRAPWLAAMAVASAAIAFSLGLQRHLPAIAALYAALWFTLNFFQGAMLAVVPDR
                     VPQEKRSLASSVVGVGAPVGALIGVNLAAWAEGETGYAALAALLIATTLAFVSLAPEQ
                     ASQSLDAPRRLKLSAALRSLNGFRSHDFAVVFALRTTMFVAQFSINNYLLYILQDHIG
                     ASNLPAGSGRIAAGALTSLRILVTIMIIFAAGWIAGRTPRRKLFAQVYALVMAAASLA
                     PIFAATWPGMLVFAALGGVAIGVYSAIDLDLMAQVLPDKNAAGRDLAVLVMAGASAQF
                     LAPTIGGGLIKLLGYNALFLFGAIGALAVGAAAFLLRGVR"
     gene            complement(5944..>6952)
                     /locus_tag="VKU03_06845"
     CDS             complement(5944..>6952)
                     /locus_tag="VKU03_06845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding protein activator"
                     /protein_id="HLJ71016.1"
                     /translation="VRVGLVLPLTQGGGPSAIGTSLRNAAALAVQESGGNDITLMVQD
                     DHSTPDGAAQATQAAIGAGADLIIGPLFAPGVREAGRVAKAAGKPVIAFSTDASVASH
                     GVYLLSFLIEGYVDRIMDYARSRGKKSFAVLAPQSEFGNVAVAEFQRNASRLGVSVVS
                     VIRYQPGQASTAASQLSAASNQIDALFIGEQAEGMPAVNDALTASGVKTQILGTGVWN
                     DARTLRLPALQGAWFASPDNSGFNAFAERYRAKFNSDPTRLATLSYDAVSLAAALARA
                     PGPDKFSDRILTNPSGFNGADGVFRFRPDGLSERGLAIMQIGNGAASVIAPAPRSFAG
                     G"
BASE COUNT         1190 a         2233 c         2307 g         1222 t
ORIGIN      
        1 ccggcggcca tctgacccgc caggttttcg cccgcgaatt gaaagtcgtc gccgccgcgg
       61 ccgggcttcg ctcgagccag atcagcccgc acgccttgcg ccacgctttc gcgagccatc
      121 tcctgcagaa cggggccgat cttcgtgtcg tccaggacct gctcggccac gccgacattt
      181 cgacgacgca gatctacacc cacgtgctcg acgagcgcgc caaggcgatg gtgagggatt
      241 tgcatccgct gagcgacgag gaagggtagg cgccgatttc cggcattctc gtcattgcgg
      301 gcgaagcgaa gcaatccatc cgtagcgttc gtcgcccggg aatggattgc tttgtcgctt
      361 tgctcctcgc catgacggta tggcgcggcg ttcgccgccc gctgtcacgc ccggaccgcg
      421 aacaattgcc gcaagctgct gagcaggcct ggctgttccc gcgtctcgcc aagcgccgat
      481 aaaaatcgct ccgcccagcg gtcgatgtcg ttctcgacaa gatgatcgta catccgctgg
      541 aagcgcgcct ttcgctcatc cagcggcatt tcgagcgcgg ccttgatcgc ggcggccatg
      601 ccttccgtct cgtgcggatt gacgaccagc gcgccggtga gctcctgcgc cgcgccggcg
      661 aattgcgaaa ggaccagcac gccgggatcg tcggcgtcct gcgccgcgat atactctttg
      721 gcgacgaggt tcatgccgtc gcgcagcggc gtcaccaggc cgacatgggc cgagcgatag
      781 aggccggcga gcatggcgcg cgaataggag cgattgacat aacggatcgg cgtccaagcg
      841 ggatcgccaa aacggccgtt gatctgcccg accagcgcgc tgacctcttg ctccatcgcg
      901 gaatattcag gcacgccggt gcggcttttg ggcgtgactt gcagaaacgt cacccggttc
      961 agccattccg gatatttttc gaggaagtga ccgaaagcct ggatgcggtg cttgatgcct
     1021 tttgaataat cgagccggtc gacgccgaga atgagccgct tgccgacaag actgttgcgc
     1081 agctcctcga ccagggtcga tttgacggag ttgcgcgcgg cgcgagcata gacggccgtg
     1141 gcgatggcga cgggaaaagc cctgacgcgt acttttcgtc cgtcgaattc gaacgagccc
     1201 ggcccgcgct ggttggcgcc gagcgctgcg aaatagcggc tgagattgtc ggcgtcctgg
     1261 tcggtctgca agccgacgag atcgtaatgg ctgagcgcgc ccatcgtctc gtcatgatgc
     1321 ggcagcgctt cgataatgtc gggcggcgga cacggaatat gcaggaaaaa gccgatcgga
     1381 ttgttgtggc cgcggccgcg caactccttg gcgaaaggca ggagatgata atcatgcacc
     1441 cagatcacgt cgtccggcgt gatgaacggc gacagcgcat cggcgaagcg gctgttgacg
     1501 cggagatacc cgccgagatc gaccgaggtg aattccgcca agtcgacgcg ataatgcagc
     1561 accggccaca ggacgcggtt ggcgaaaccg ttataatatt cctggaaatc gttctccgaa
     1621 agatcgagcg tgatgtacgt gcgccggcgc cgcaggatga ccgaggcggc cggcgtctcg
     1681 ccttccttgc tgaccttgcc gctccagccg aaccatacgc cttccctttg cttcagcgcg
     1741 gcgttgacgg cgacggtgag gccgccggcc tggcgcggca cgccggcgac cggaacattg
     1801 acccgattcg agacgaggat cagacggggc gccatgccgc ctcccacttg atcgacagcc
     1861 gcatcgccga gtcgatgatc gccgccatcg aataggtttg cggaatattg ccccacagaa
     1921 cgccggtttc cgggtgtatg tcctccgaga aaatgccgaa tgcgtttcgg cgctcgagca
     1981 aatctgagaa gagttcgcgc gcttcgtcgc gccggccgat ctcggcgagc gcgtcaatat
     2041 accagaaatt gcaaaccagg aacgccgttt ccggccagcc gaaatcgtcg cgcgccgtat
     2101 agcgcatgat gcggccgctt ctcatcaagt cgcggccgat cgcttcgcac gtgctgacga
     2161 agcgcggatc gtcggcggcg atcaagccga gctcggcgag gagcagcacg ctggcgtcga
     2221 gctcgtcgcc ttcgaacgcg ccgacgaaag cctttttgtc ttcgcgccag gcgcgcgtga
     2281 aaatttcctg gcgcagcgaa gcggcgcgct cggcccagag caacgcacgc tcgtgattgc
     2341 cgagccggcg cccgatctgc gccaggcggt cgcaagcggc ccagcagatc gagaccgaat
     2401 gcgtgtggat gcgctcgcgg ccgcgatatt cccagatgcc ggcgtccggc ttcaaggcga
     2461 gccgcatcgc tctctcgccg agcggctcaa ggcggcgata gagagattcg tcgcccatgc
     2521 gcggcagccg ctcgtcgatg aacatctgcg tcgcggcgag aatcgcgctg ccatagacat
     2581 cgtgctgatg ctgctcggct gcctgattgc cgacccggac cgggccttgg ccgagaaatc
     2641 cggcaaggtt gggcgcgatc tcctcgaaaa gcgattcgcc cggcacgacg gaatggaccg
     2701 gccgcaacgg cgcatcggtt tcggtgacga tggtcatgat gtagttgaga taggcctcca
     2761 tcgtgtcggt cgcgccgagc ttgttgaggg cctgggtgac gaaataagcg tcgcgcagcc
     2821 agcaatagcg gtagtcccag ttccggccgc tattggccgc ctcgggaatc gacgtcgtgt
     2881 gagccgcgac aatgccgccg gtctcttcga agctgcacat tttcagcgtg atcgcggcgc
     2941 gcatcacctc gttctgccat tcgtagggga tggcgagggt gcgcgtccaa tgcagccagt
     3001 aattgcgcgt gagctcctca aattcgttcg cagtggcctc gaaggcgctg cggaacggct
     3061 cgtccggccc gagcacgagc gtcatcggcc gggtcagcgg gaacgtcgtc tcctgcgcca
     3121 cataggagag cggcgcgtcg gtgttcaggc ggatgaccgt gcccgcgcct tgatagcgga
     3181 tatggttcga gccgatcgaa aggtccgaca tcggccggcc ataggcctcg gtcggacgaa
     3241 ccctgatcgt gatcctcggc tggccggcga ggggcgtgat gcggcgcacc aattgcggcg
     3301 gatggaatgt gcgctcgtaa cgctggaaac gcggcgcgaa atcggtgatc cgcaagcgtc
     3361 cgccctgctt gtcggtgagc tcggtctcga ggattgcggt gttgcggaca tacgcggacc
     3421 gcgcatcgac gagatcgttg agaacaacgt cgcaaaagcc tttttcctcg tcgccggcga
     3481 gcagtcggca aaagatcgga ttggagtcga agcgcggaaa gcaccaccag acgatccgcc
     3541 ccaccggatc gaccaaagcg gcggtgcggc aattgccgat cagagccaga tcaagcgcct
     3601 gcttgcccgg ccggcgccgg ctcttcgcgt cggtctcgct catccgccca acctttcgag
     3661 ccatgcgcgc aagtccttag gacttgcaaa ccaacccgac acgccgggca gctcggcgcc
     3721 gacggagaga gccaagccgc ccagcgcaac cgccgtttca aagcccggcc ggtcgacgcg
     3781 atcgtcggcg acgaaaatcg gccgccggcc ggcgaaaggc ggccgcgcca tgaagcactc
     3841 gatcgccttg cctttatcga acccggcaag cttgatctca taaaccgagc ggccgccgat
     3901 cacctccagc ggcaattccg gatattcgtg caggaattgc tgaagcgcct tgcaaatgtc
     3961 ctcggcgacc gggccggcga gattgtaatg aacggcgaag ctcagcccct tgttctcggc
     4021 gaacgcgccg ggaaatctcg caacgatgcg cttcagccgc atccaggcgt tttcgggcaa
     4081 tcgccccgtc gccagggact ttccgggctc gttgggcgca tagcggattt cgccgccatg
     4141 cacgccgctg gctctcagcc gcaagggctt gaacaaccgg tccagctcct cgatcggccg
     4201 gccgctgacc agagccagcg cgccgtcgag gcggcgttcg gcggaagcga gctcgcgcaa
     4261 caacgcggcc ggaacatcga ccgcgtcggg cgtcggcgcc aagtcgataa tcgttccgtc
     4321 cacgtcgagg aacaggccaa tcgagtgtcg atccaaagtc gccggctcgg gcgccgtcat
     4381 ccacctgcct ttcaatttgg gacaaagctt caacgccggg gaccgggtgt caatcttgac
     4441 tcttttacgt tggggagaat tcgcgcagac gcgcgaaaaa gcacttaata actttcatgc
     4501 tccgggcgaa gttccgcagt aacgcgattg tgaacgcggc gctgagtgag gtcgctagga
     4561 gcgaaacagg acaagcgaga gaaccgctct tcggcgctcg ctgattcgcg acaccgcgcc
     4621 gaacgggcga gaaagtacca atgcgggcgg aaacgcgtca aggacgcgag gactgcgtcg
     4681 ccgccgatcg gctccagcct tcggccgccg cggcgcggca gcgccccgac gccgtcggcc
     4741 ttcagcgtct cttgttcttt ctgttgccgg cggccttcgc gctttacgcg aatttccagg
     4801 gtgttcagct catcctgatt cccgctcaag tggaaaccgt cgagccggcg cgcaagatcg
     4861 acaatctcgc gttgcttacc gtgttctgcg cgatcggcgg cgtcatcggc ctgatggccg
     4921 gcggcggcgt gagcgacgcg acgcggagcc ggtggggccg ccgcgcgccc tggctcgccg
     4981 cgatggcggt tgcctcggcg gcgatcgcct tttcgctcgg actgcagcgc cacctgccgg
     5041 cgatcgccgc gctttacgcc gctttgtggt tcacgctcaa tttcttccag ggcgcgatgc
     5101 tggcggtcgt gcccgatcgc gtgccgcagg agaagcgcag tctggcctcc tcggtcgtcg
     5161 gcgtcggcgc gccggttggc gcgctgatcg gcgtcaacct cgccgcctgg gcggagggcg
     5221 aaaccggcta tgcggctctg gcggcgctgc tgatcgccac caccctggcc ttcgtgagcc
     5281 tcgcgccgga gcaggcgagc caaagcttag acgcgccgcg tcgcttgaag ctgagcgccg
     5341 cgctgcgctc gctcaacgga ttccgctctc acgactttgc cgtcgtcttc gccttgcgga
     5401 cgacgatgtt cgtcgcgcaa ttttcgatca acaattatct gctctacatc cttcaggatc
     5461 atatcggcgc gtcgaacctg ccggcaggaa gcgggcggat cgcggccggc gcgctgacct
     5521 cgctgcgcat cctcgtcacg atcatgatca ttttcgccgc cggctggatc gccgggcgta
     5581 ctccgcggcg gaagctgttc gcgcaagtct acgcgctggt catggcggcg gcgtcgttgg
     5641 cgccgatctt cgccgcgacc tggcccggca tgctcgtttt cgccgcgctg ggcggcgtcg
     5701 cgatcggcgt ctattcggcg atcgatctcg atctgatggc gcaggtcctg cccgacaaaa
     5761 acgccgccgg acgcgatctc gccgtgctgg tcatggccgg cgcgtcggcg caatttctcg
     5821 cgccgacgat tggcggcggc ctgatcaagc tgctcggcta caacgctctc ttccttttcg
     5881 gcgccatcgg cgcgctcgcc gtgggcgcgg cggcgttcct gttgcgcggc gtacggtaaa
     5941 gcctcacccg ccggcgaagc ttcgcggcgc cggcgcgatc accgaggcgg cgccgttgcc
     6001 gatctgcatg attgccaggc cgcgctcgga gagcccgtcg ggccggaagc ggaagacgcc
     6061 gtcggcgccg ttgaagcctg acgggttggt caggatccgg tcggaaaact tgtccggccc
     6121 cggcgcccgg gcgagggccg ccgcgagcga cacggcgtcg taggagagcg tcgccaggcg
     6181 cgtcggatcg ctgttgaact tggcgcggta gcgctcggcg aacgcgttga agccggaatt
     6241 gtcgggcgag gcgaaccagg cgccttgcag ggcgggcagg cgcaaggtgc gcgcgtcgtt
     6301 ccagacgccg gtgccgagaa tttgcgtctt gacgccgctc gcggtgagcg cgtcgttgac
     6361 ggcgggcatg ccttcggcct gctcgccgat gaacagagcg tcgatttggt tggaggcggc
     6421 cgaaagctgc gaggccgcgg tcgaggcctg gccaggttga tagcggatca cgctgacgac
     6481 cgagacgccg agccgcgagg cgttgcgctg gaattcggcg accgcgacat tgccgaactc
     6541 gctttgcggc gccagcacgg cgaaggattt tttgccgcgc gaccgggcgt aatccatgat
     6601 ccggtcgaca tagccttcga tcaggaagga caaaagatag acgccatgcg aggcgacgct
     6661 ggcgtcggtc gaaaaggcga tgaccggctt gccggcggct ttggcgacgc gcccggcctc
     6721 gcgcacgccc ggcgcaaaga gcgggccaat gatgagatcg gcgccggcgc cgatcgcagc
     6781 ctgcgtcgct tgcgccgcgc cgtcgggggt cgagtgatcg tcctgcacca tcaaggtgat
     6841 gtcattgccg ccggattcct gcacggcgag cgcggcggcg ttgcgcaagg acgttccgat
     6901 cgcgctcggt ccgccgccct gggtcagcgg caggacgagg ccgacgcgga cc
//
LOCUS       DATGJW010000215         2169 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1490115, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000215 DATGJW010000000
VERSION     DATGJW010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2169)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2169)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2169
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1490115"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            199..474
                     /locus_tag="VKU03_06850"
     CDS             199..474
                     /locus_tag="VKU03_06850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013500748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6460 domain-containing protein"
                     /protein_id="HLJ71017.1"
                     /translation="MASPHLEHFLGGSPAAVAIRLIVVSFVVGVLLVMFGVEPWDIVA
                     SIERLLRRVAEYGLADFRQFGRTLLTGAIVVVPIWLLIRLLDSRRSR"
     gene            complement(669..1733)
                     /locus_tag="VKU03_06855"
     CDS             complement(669..1733)
                     /locus_tag="VKU03_06855"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HLJ71018.1"
                     /translation="MIGALAALARPLLFSLEPETAHRLAIAALSFAPGAPAAPDPRLA
                     TQAFGLAFPNPLGMAAGFDKNAEVPDGLLGLGFGFVEVGTLTPLPQAGNPRPRLFRLA
                     RDSALINRLGFNNQGYQAAHRRLAARAKRGIVGVNIGPNKDSADRVADYARGIAAFAD
                     VASYFTINVSSPNTPHLRDLQQRSALDELVARVIEAREACAERRPILVKIAPDIDLAA
                     LDDIVAVARRRGTDGMIVANTTVARPPGLADPAAQEAGGLSGRPLFAPSTRLLAQTYL
                     RAEGALPLIGCGGIDSAAAAYAKIRAGASLVQLYSALIFRGPALVGEILAGLRKLLDA
                     DRRSTIAEAVGTNARDFAGA"
     gene            complement(1730..2074)
                     /locus_tag="VKU03_06860"
     CDS             complement(1730..2074)
                     /locus_tag="VKU03_06860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007695608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF952 domain-containing protein"
                     /protein_id="HLJ71019.1"
                     /translation="MPLIFKIVAAAEWRAAEQKGAFEGSAVDRADGYIHFSTAEQAIE
                     TAAKHFAGQDGLILVAIDADALGPALKWEPSRGGALFPHLYAAMPLAAVRWIRPLPIG
                     ADGRHVFGTLAE"
BASE COUNT          337 a          710 c          777 g          345 t
ORIGIN      
        1 ctcgtcggct atatcggctg gaaggacttg atgcgggtgc gcgccaagct gcgcagcgaa
       61 gagcggcggc gcaaggtctt ctttcgcatc cggccgatct ccgccaatta gcggcggcgc
      121 gggctcagcg ctgatttcga cctgcggcct tcgatatgct agaggcgcgc cagccgcgcc
      181 tctcgaagga ttcgcgccat ggccagtccc catctcgaac atttcctcgg cgggtcgccg
      241 gccgccgtcg cgatccggct gatcgtcgtc tcattcgtcg tcggcgtgct gctcgtcatg
      301 ttcggcgtcg agccgtggga catcgtcgcc agcatcgagc ggctcctccg ccgcgtcgcc
      361 gaatacggcc tcgccgattt ccgccaattc gggcggacgc tgctgaccgg cgcgatcgtc
      421 gtcgtgccga tctggctgct catccgcctg ctcgattcgc gccgctcgcg gtgagctatc
      481 ctcccccgtg gagcgaagcg gaacggggga ggggggaccg cccgaagggc ggtggagggg
      541 gctgccggcg gttgagaatt atcctgagag gccggagccc cctccaccat gcttcgcatg
      601 gtctccctcc cccgctttcg cgggggagga aagggcggcg gcgctgaaag tacgttatga
      661 cgaaatggtc acgcgccggc aaaatcccgc gcgttcgtcc cgaccgcctc ggcgatcgtg
      721 ctgcgccggt cggcgtcgag cagcttgcgc aagcccgcca ggatttcgcc gaccagcgca
      781 ggcccgcgaa agatcagcgc cgaatagagc tggactaagc tcgcgccggc gcggattttg
      841 gcgtaggcgg cggcggcgct gtcgatcccg ccacagccga tcagcggcaa cgcgccctca
      901 gcgcgcagat aggtttgcgc gagcagccgc gtcgagggcg cgaaaagcgg ccggccggaa
      961 aggccgccgg cttcctgcgc ggcgggatcg gcgaggcctg gcggccgagc caccgtcgtg
     1021 ttcgcgacga tcatgccgtc ggtcccgcgg cgtcgcgcca ccgcgacgat gtcgtcgagc
     1081 gccgcaaggt cgatgtccgg cgcgatcttg acgaggatgg ggcggcgctc ggcgcaggcc
     1141 tcgcgcgcct cgatcacccg ggcgacgagt tcgtccagcg cgctcctttg ctgcaggtcg
     1201 cgcagatgcg gcgtattggg cgaggagacg ttgatcgtaa agtagctcgc gacatcggcg
     1261 aaggcggcga tcccgcgcgc atagtccgcg acgcggtcgg cggaatcctt gttcggcccg
     1321 atattgacgc cgacaatgcc gcgctttgcg cgcgccgcga ggcgccgatg cgcggcttgg
     1381 tagccttgat tgttgaagcc aagccgattg atcagcgccg agtcccgcgc cagccggaac
     1441 aggcgcgggc gcggattgcc ggcctggggc aaaggggtca gcgtgccgac ctcgacaaag
     1501 ccgaagccga ggccgagcag gccgtccggg acttcggcgt tcttgtcgaa gcccgccgcc
     1561 atgccgagcg ggttgggaaa ggcgaggccg aaggcttgcg ttgcgaggcg tggatcgggc
     1621 gcggccggcg cgcctggcgc gaaggacaac gccgcgatcg ccaggcgatg agcagtttcg
     1681 ggctcaagcg agaacagcag cggccgcgcc agcgcggcga gcgcgccgat cattcggcga
     1741 gcgtcccgaa aacatgccgg ccgtcggccc caatcggcag cggccggatc cagcgcaccg
     1801 ccgcaagcgg catggcggcg tagagatgcg ggaacagggc gccgccgcgc gagggctccc
     1861 atttcaaagc cggcccgagc gcgtcggcgt cgatcgcgac gagaatcaag ccatcttggc
     1921 cggcgaaatg tttcgccgcc gtttcgatcg cctgctcggc ggtggagaag tgaatatagc
     1981 cgtcggcgcg atcgacggcg gagccttcga atgcgccctt ttgttcggcg gcgcgccatt
     2041 ccgccgccgc gacgatcttg aagatgagcg gcatggggtt caggccgtca agcgatccat
     2101 gcggtcatcg cgtaatgacg gggggctcat tcgagcagca tgcgcttgag caattcgagc
     2161 tcttcgttg
//
LOCUS       DATGJW010000216         5124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_351767, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000216 DATGJW010000000
VERSION     DATGJW010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5124
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351767"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VKU03_06865"
     CDS             complement(<1..533)
                     /locus_tag="VKU03_06865"
                     /inference="COORDINATES: protein motif:HMM:NF006622.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA-binding protein"
                     /protein_id="HLJ71020.1"
                     /translation="MRQADREGSERTCAATGRKGPPEAMIRFCLSPQGAVVPDLRRKL
                     PGRGVWVFARKAAVAQAAHKQVFARGFKAEARAEPDLADLVERLLEQEALQFLSIVNK
                     AGLVVAGAFKVEEAIRGGGIAALINAAEASKQGMAKLERLIKARLGAEAEKVRRINLF
                     SSSQLDLALGRSNVIHAA"
     gene            complement(553..2166)
                     /gene="nusA"
                     /locus_tag="VKU03_06870"
     CDS             complement(553..2166)
                     /gene="nusA"
                     /locus_tag="VKU03_06870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266595.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HLJ71021.1"
                     /translation="MAVSANRLELLQIVDAVAREKSIDRSIVLASMEDAMQKAARSRY
                     GQETEVRAEINPKTGEIRFSRLLLVVEQVENDATQISLAEARKKNPAAQIGDWIAESL
                     PPFDFGRIPAQSSKQVLVQKIREAERDKQYEVYKDRIGEIINGVVKRVEYGNVVVDLG
                     GAGAGEAVVRRDELIPREMFRPGDRIRAYVYDVRREQRGPQIFLSRTHPQFTAKLFAQ
                     EVPEIYDGIVEVKSVARDPGSRAKIAVISNDSSIDPVGACVGMRGSRVQAVVNEQQGE
                     KIDIIPWSADAATFIVNALQPAEVMKVVLDEDSTRIEVVVPDDQLSLAIGRRGQNVRL
                     ASQLTGWDIDILTEAEESERRQKEFVERTNVFMSALDVDEVVAQLLASEGFRSVEELA
                     YVEPRELASIEGFDADTAAEIQARAQAHLARIEAEHDEKRKALGVADELKDVDGLTTA
                     MLVKLGENDVKTIEDLAGCATDDLVGWTERSEGEAKQHPGFLDGFDLSREQAEAIVMR
                     ARVMAGWIEPPAESEAGEPAEQAAEEEAQ"
     gene            complement(2170..2868)
                     /gene="rimP"
                     /locus_tag="VKU03_06875"
     CDS             complement(2170..2868)
                     /gene="rimP"
                     /locus_tag="VKU03_06875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimP"
                     /protein_id="HLJ71022.1"
                     /translation="MKAARDERPDPLDEARLIEESGPARRIAQIVGPTLRGLGLRLVR
                     VKISAAAGCTVQIMAERGDGSMSIEDCEAASAALSPVLDVEDPIKQAYRLEISSPGID
                     RPLVRVSDFQRAVGHEAKVEMARPIDGRKRFRGVIESLDAGEHPSAYMRVAAAPGGED
                     AIVALPIDEMGEARLVLSDALIREALRREKAAKKNAKQARKRAKASKDEEAPQALIRT
                     KEAEALAASGDKET"
     gene            complement(3061..3552)
                     /locus_tag="VKU03_06880"
     CDS             complement(3061..3552)
                     /locus_tag="VKU03_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein N-terminal
                     domain-containing protein"
                     /protein_id="HLJ71023.1"
                     /translation="MSALIRLAKASSRFVACGALAVAMLAGFGVAGAVAEPFINVSID
                     QAKIAKVPEGTKTLVIGSPIVADVTLLHGSSTMVVTGKGYGETNLIALDGSGNVLQER
                     MIRVEPTGSVLVVQRGMERESYSCTPKCMPVVELGDGSSFSQTSGQITSRNALAAPQQ
                     GAK"
     gene            3730..4419
                     /locus_tag="VKU03_06885"
     CDS             3730..4419
                     /locus_tag="VKU03_06885"
                     /EC_number="4.1.2.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydro-3-deoxy-6-phosphogalactonate aldolase"
                     /protein_id="HLJ71024.1"
                     /translation="MNSRFAEAMAKLPLAAILRGIRPDEATPILMALIDAGFHIIETP
                     LNSPQPFETIRLMRLVAPKDALIGAGTVRTESEVAAVAEAGGDLIVMPHADVAIVKAA
                     KARGLIALPGVATPTEAFAALDAGADALKAFPAEMIQPAILKAWRAVIPTDVPILPVG
                     GIAPASMAPYVAAGADGFGLGSALYKPGDGAKKVAENAAAFVAAWRKIKPPAAKSLPR
                     AQPFTLKIDAP"
     gene            complement(4386..>5124)
                     /locus_tag="VKU03_06890"
     CDS             complement(4386..>5124)
                     /locus_tag="VKU03_06890"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HLJ71025.1"
                     /translation="DEKLLHRDVRTVWGDGLGDYVAEPGLGPDGGVVWREPGQASGDE
                     SVLRDAKNPFQRTGGLRVLDGPLGRAVIKTSAVAAERHVIEAPARVFHSQEELHAAFK
                     AGELFRDLVAVVRFQGPSANGMPELHKLMPPLGVAQDRGFKVALVTDGRLSGASGKVP
                     AAIHVTPEAAAGGPIAKIRDGDLLRVDAPAGRLDVLVDEKEWAARPNETYDLSAAHRG
                     VGRELFAAFRACVGKADQGASIFSVNG"
BASE COUNT          830 a         1776 c         1609 g          909 t
ORIGIN      
        1 gctgcatgta tcacatttga cctccccaac gccaaatcca attggcttga agagaaaaga
       61 tttattcgcc tgactttttc cgcctccgcg cccagtctcg ccttgatcag gcgctccagc
      121 ttcgccatcc cctgttttga cgcttcggcc gcgttgatca gcgcggcaat cccgccgccg
      181 cgaatcgcct cctccacctt gaacgcgccg gcgacgacaa ggccggcctt gttcacgatc
      241 gagagaaact gcagcgcctc ttgctcgagc aggcgctcga ccagatcggc caaatccggc
      301 tcggctctcg cctcggcttt gaagccgcgc gcgaaaacct gcttgtgcgc ggcttgcgcc
      361 accgccgcct tgcgggcgaa cacccagacg ccgcggcccg gcaatttgcg gcgcagatcc
      421 ggcacgaccg cgccctgcgg cgacaggcaa aagcggatca tcgcttcggg cgggcccttc
      481 cggccggtcg cggcgcaagt ccgctccgat ccctcgcgat ccgcttgacg caaatctgcc
      541 tccgcgacgc gtctattgcg cctcctcctc tgcggcttgt tcagccggct ctcccgcctc
      601 gctctcggcc ggcggctcga tccagcccgc catcacccgg gcgcgcatga cgatcgcctc
      661 ggcctgctcg cgcgacaggt cgaatccgtc gaggaagccg ggatgttgct tggcctcgcc
      721 ttcgctgcgc tcggtccagc cgacgagatc gtcggtggcg caaccggcga ggtcctcgat
      781 cgttttcacg tcgttttcgc cgagcttgac cagcatggcg gtggtcagcc cgtcgacatc
      841 cttcaattcg tcggcgacgc cgagcgcctt gcgcttttcg tcatgctccg cctcgatcct
      901 ggcgagatga gcctgggcgc gcgcttgaat ctccgccgcc gtatcggcgt cgaacccttc
      961 gatcgaagcc aattcgcgcg gctcgacata agcgagctcc tcgaccgagc ggaacccttc
     1021 cgaggcgagc aattgcgcca cgacctcgtc gacgtcgagc gcgctcatga acacgttggt
     1081 gcgctcgacg aactccttct gccgccgctc cgattcttcc gcctcggtca ggatgtcgat
     1141 gtcccagccg gtcaattgcg aggcgaggcg cacgttctgg ccgcgccggc cgatcgccag
     1201 cgaaagctga tcgtcgggaa cgacgacttc gatgcgcgtc gaatcttcgt cgagcacgac
     1261 cttcatcact tcagccggct gcagcgcatt gacgatgaac gtcgccgcgt ccgccgacca
     1321 ggggatgatg tcgatcttct cgccctgctg ctcgttaacc accgcctgca cgcgcgagcc
     1381 gcgcatgccg acgcaggcgc cgaccggatc gatcgaggaa tcgttggaga tgaccgcgat
     1441 cttggcgcgc gagccgggat cgcgcgccac cgacttcacc tcgacaatgc cgtcgtaaat
     1501 ctccggcact tcctgcgcga acagcttggc ggtgaattgc ggatgggttc gggagaggaa
     1561 aatctgcggc ccgcgctgct cgcgcctgac gtcgtagaca taggcgcgga tgcgatcgcc
     1621 cggccggaac atttcgcgcg ggatcagttc gtcgcgccgc accaccgctt cgccggcgcc
     1681 ggcgccgccg agatcgacga ccacgttgcc gtattcgacg cgcttgacga cgccgttgat
     1741 aatctcgccg atccggtcct tatagacctc gtattgcttg tcgcgctcgg cctcgcgaat
     1801 tttctgaacg agcacttgct tggaggattg cgccgggatg cgcccgaaat cgaacggcgg
     1861 cagcgattcg gcgatccagt cgccgatctg cgccgcggga ttcttcttgc gcgcctcggc
     1921 gagggaaatc tgcgtcgcgt cgttctcgac ctgctcgacg acgagcagca gccgcgaaaa
     1981 cctgatctcg ccggttttcg gattgatctc ggcgcgaacc tcggtctcct gcccatagcg
     2041 cgagcgcgcc gccttctgca tcgcgtcctc catcgaggcg agcacgatcg agcggtcgat
     2101 cgatttctcg cgcgcgacgg cgtcgacgat ctgcaagagt tcgagcctat tggcgctgac
     2161 ggccatgtcc tacgtctcct tgtctcccga agcggcgagc gcttcggcct cttttgtcct
     2221 gatgagcgct tgcggcgctt cttcgtcttt cgaagccttc gcccgcttgc gcgcctgctt
     2281 ggcgttcttc ttggcggctt tctcgcgccg caacgcctcg cgaatcaaag cgtcggatag
     2341 aacgagccgc gcttcgccca tctcgtcgat cggcagcgcg acgattgcgt cttcgccgcc
     2401 cggcgcggcg gcgacgcgca tatacgcgct cggatgctcg ccggcgtcca agctttcgat
     2461 cacgccgcga aagcgcttgc ggccgtcgat cggccgcgcc atttcgactt tcgcctcatg
     2521 gccgacggcg cgctggaaat ccgatacgcg aaccagcgga cgatcgatgc cgggcgagga
     2581 aatctccagc cgataggctt gcttgatcgg atcctcgaca tcgagcacgg gcgacaaggc
     2641 ggcgctcgcc gcttcgcaat cctcgatgct catcgagccg tcgccgcgct ccgccatgat
     2701 ctgcacagtg cagccggcgg ccgccgaaat cttcacccgg acgaggcgca agccgaggcc
     2761 gcgcaaagtc ggcccgacga tctgggcgat ccgccgcgcc ggaccgctct cctcgatcag
     2821 gcgcgcttcg tccaacggat cgggacgctc gtcgcgagcc gctttcacat caatcgccaa
     2881 tcggccttcg gccatcgaac ctgccggcaa taaaaaagag cgggtcccgg ccggggccca
     2941 ctctcaaaaa tcttctcatc acgatgagaa gctatgagaa accaactgtg ctctatttag
     3001 aggtttggac ggtgataagc aagggcgcag gcgatcattt ttgcgccggc gacagccgcg
     3061 ttatttggcg ccttgctgag gcgccgcgag ggcgttgcgc gaggtgatct ggccggatgt
     3121 ctggctgaag gaggagccgt cgcccagctc gacgaccggc atgcatttag gcgtgcagga
     3181 ataggattcg cgctccatgc cgcgctgcac gaccaggacc gagccggtcg gctcgacgcg
     3241 aatcatccgc tcctggagca cgttgcccga gccgtcgagg gcgatgagat tggtttcgcc
     3301 atagcccttg ccggtcacga ccatggtgct ggagccgtgc aggagggtca cgtcggcgac
     3361 aatcgggctg ccgatcacca gagtcttggt gccctccggc actttggcga ttttggcctg
     3421 gtcgatcgag acattgatga acggctcggc gaccgcgccg gcgacgccaa atccggcgag
     3481 catggcgaca gccagcgcgc cgcaggcgac gaagcgactc gacgcttttg ccaagcggat
     3541 caacgcggac atggctctct ctcccgcggg aagggcggct ttgcgcattt catccagaaa
     3601 tggtgaagga atgctgaatc ggggaatggc gaatggaaac cacactcgcc attcgccact
     3661 tcgccacgcg ctatattgcg caggcctccg tttcccgcca aaaagagagg cgtttcggaa
     3721 ggagagccct tgaacagccg ctttgccgag gcgatggcga aactgccgct cgccgcgatc
     3781 ctgcgcggaa tcaggccgga cgaggcgacg ccgatcctga tggcgctgat cgatgccggc
     3841 ttccacatca tcgagacgcc gctcaactcg ccgcagcctt tcgagacgat ccgcctgatg
     3901 cgcctggtcg cgccgaaaga cgcgctgatc ggcgcgggga cggtcaggac ggaaagcgaa
     3961 gtcgccgcgg tcgcggaagc cggcggcgac ctcatcgtca tgccgcatgc cgatgtcgcg
     4021 atcgtcaagg cggccaaggc gcgcggcctg atcgcgctgc cgggcgttgc gacgccgacc
     4081 gaagccttcg ccgcgcttga cgccggcgcg gacgccctca aagcgtttcc ggcggaaatg
     4141 atccagccag ccatcctgaa ggcctggcgg gcggtgattc cgaccgacgt tcccattctg
     4201 ccggtcggcg gaatcgcgcc cgcttcgatg gccccctatg tcgcggccgg cgccgacggc
     4261 tttggcctcg gctcggcgct ctacaagccc ggcgacggcg ccaagaaagt cgccgagaac
     4321 gccgccgcct tcgtcgccgc ctggcgcaag atcaagccgc ccgccgccaa gagcctgccg
     4381 cgcgctcagc catttacgct gaaaatcgac gcgccttgat cggccttgcc gacgcacgcc
     4441 cgaaacgcgg cgaagagttc gcggccgacg ccgcgatggg cggctgaaag gtcgtaggtt
     4501 tcgttgggcc gcgccgccca ttccttttcg tccaccagca cgtcgaggcg gccggccggc
     4561 gcgtcgacgc gcaacaggtc gccgtcgcgg atcttggcga tcggcccgcc cgccgcggct
     4621 tccggcgtca cgtgaatggc ggccggcacc ttgcccgacg cgccggagag ccgtccgtcg
     4681 gtgaccaggg cgactttgaa gccgcgatcc tgggcgacgc cgagcggcgg catcagcttg
     4741 tgcagttccg gcatgccgtt ggcgctcggc ccctggaacc gcacgacggc gacgaggtcg
     4801 cggaagagct cgccggcctt gaaggcggcg tgcaattcct cctgcgaatg gaaaacgcgg
     4861 gccggcgcct cgatgacgtg ccgttcggcg gcgaccgcgg acgtcttgat caccgcgcgg
     4921 ccgagcgggc cgtcaagaac gcgcaggccg ccggtgcgct ggaacggatt tttggcgtcg
     4981 cgcaagacgc tctcgtcgcc gctcgcttgg cccggctcgc gccagacgac cccgccatcc
     5041 ggcccgaggc ccggctcggc gacgtaatcg ccgagcccgt cgccccacac cgtgcgcacg
     5101 tcgcgatgca gcagcttctc gtcg
//
LOCUS       DATGJW010000217         4434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_351908, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000217 DATGJW010000000
VERSION     DATGJW010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4434
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_351908"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(195..271)
                     /locus_tag="VKU03_06895"
     tRNA            complement(195..271)
                     /locus_tag="VKU03_06895"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(235..237),aa:Arg,seq:cct)
     gene            381..1181
                     /locus_tag="VKU03_06900"
     CDS             381..1181
                     /locus_tag="VKU03_06900"
                     /inference="COORDINATES: protein motif:HMM:NF012774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thermonuclease family protein"
                     /protein_id="HLJ71026.1"
                     /translation="MVAALFALAAAEGRAAPCGAGPAQQVAVKAVAERLEITLADGRQ
                     VRLAGLAAPDPDHGDPATAAAALAFAKDWLAGREVGLRLLYAKPDRWGRLPVDLLAPP
                     PGEAGAAPVSVAAFLLRSGYARVWPEPDAYACLADLMRAEAEARDKRLGLWRDPYYGV
                     VAAADLDNLRKRDGQFTLVEGTPSRVGEGRSRYYVDFGLHRGFTVVIPKRRAKVFERA
                     GMTIAALAGAKIRVRGALDNRFGLRMEISDPDGIERLGESAKGEAQKP"
     gene            1178..2665
                     /locus_tag="VKU03_06905"
     CDS             1178..2665
                     /locus_tag="VKU03_06905"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M48 family metalloprotease"
                     /protein_id="HLJ71027.1"
                     /translation="MILGLSERARGGARRLAAAAVLLAPFLILGCANLDNSKSVASLV
                     PSAAPRTTGGAAAAAAAERRKLVDLFGGEYSAPVAERYLNGVLTRLAAASPSPSAPYR
                     VTILNSPIVNAFALPSGDIFVTRGLLALANDTSEIAAVMAHEIAHITARHAAKRAELV
                     KTAALFSRVSTDVLRRPQEGQEEEAKAKLSLAHFSREQEFEADKIGIKDTAAAGYDPY
                     AASRFLASLGRSTALRAALLGEATSAKPDIMSTHPSTPERVEAALQEARAIGPPGTGD
                     SGRDAYLSAIDGVVFGDDSTQGLVRGTRFIHPKLGFAFNAPDGFVLDNQSAALIGVGD
                     SGAQALRLDSIMVDPATGLDTAIASGWIDGVKTSSVQKTTVNDLPAATAIAQGDQWSF
                     RLGAVRIGDKVYRLIFAAHALTPSVDERFRASLSSFHRLNLQEASTIHPAHIEIVTAQ
                     AGDTADSIAARMAVSDRPLEHFLLLNGLEKGGPLQSGQRYKIVVE"
     gene            2756..3400
                     /gene="rdgB"
                     /locus_tag="VKU03_06910"
     CDS             2756..3400
                     /gene="rdgB"
                     /locus_tag="VKU03_06910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RdgB/HAM1 family non-canonical purine NTP
                     pyrophosphatase"
                     /protein_id="HLJ71028.1"
                     /translation="MAHRKISGRLVAATHNSGKLRELRDLLGPYSVEVVSAGALGLPE
                     PDETGENFVANAEIKAMAAARAAGLPALADDSGLCVDALDGAPGIYSARWAGGGKDFS
                     GAMRRVEDELRKKNAPPPWRAYFVSALALAWPDDHIERFEGRVDGALVFPPRGAHGFG
                     YDPIFVPDGHRLSFGEMTAEAKHGLPADGSRALSHRARAFQDLARACLDRAAAR"
     gene            4009..4374
                     /locus_tag="VKU03_06915"
     CDS             4009..4374
                     /locus_tag="VKU03_06915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71029.1"
                     /translation="MAVAWPANGAPVDETDARQLRCVGDALAVYADRLADVAPILPEG
                     MRTIPAAVKAAARSVRVAKTKTEAAAAIATATAVVHKAITLIKADDPIVRSIATREAG
                     EMTKTLDLADAKLEKAVGL"
BASE COUNT          697 a         1477 c         1514 g          746 t
ORIGIN      
        1 ttttcgattc gatcggcaac cagcgcgacg acatgcgctt ccgcttcggc tttggtcttg
       61 ccgtaggcga gaactccagg cagcgcgaac ctccgcatcc tcacgctcga acccaatctt
      121 gaacattgtc catatctgag cttcgagaaa agaaatgcgc ccttttcaat ggcccgcaac
      181 gggcggcgct gaaatggcgt cccggaaggg agtcgaaccc ctgacctacg gtttaggaaa
      241 ccgttgctct atcctgctga gctaccggga cgcattgacg cggcgcgttg ggcccgcaag
      301 atgccgcgat ggctcgggcg ggcagggcgc gtctaacacg aaaattccgc cggcgcatcc
      361 ccgcgcgggt ggtcggcgcc gtggtcgcgg ccctgtttgc gctcgccgcc gccgagggcc
      421 gcgccgcgcc ctgcggcgcc ggtccggcgc agcaggtcgc cgtcaaggcg gtcgccgagc
      481 ggctcgaaat cacgcttgcc gacggacggc aggttcggct cgccgggctt gccgcgcctg
      541 atccggatca cggcgatccg gcgactgccg cggctgcgct ggccttcgct aaagattggc
      601 tcgcgggccg cgaggtcggc ttgcggctgc tctacgccaa gccggaccgc tggggacgcc
      661 tgccggtcga tcttctcgcg ccgccgccgg gcgaggccgg agcggcgccg gtctccgtcg
      721 ccgcctttct gctgcgctcc ggctacgccc gggtctggcc cgagcccgac gcctatgcct
      781 gcctcgccga cctcatgcgg gccgaggcgg aagcccgcga caagcggctt ggcctctggc
      841 gcgatccata ttatggcgtc gttgcggcgg ccgatctcga caatttacgg aagcgtgatg
      901 gccaatttac gttggtcgag ggaacgccgt cgcgagtcgg agagggccga agccgctatt
      961 acgtcgattt cggccttcat cgagggttca cggtcgtgat cccaaaaagg cgggcgaaag
     1021 tattcgagcg tgcgggaatg acgatcgcgg cgttggccgg cgcgaagatc agggtccgcg
     1081 gcgcgctcga caaccgtttt ggattgcgca tggagatttc ggacccggac gggatcgaac
     1141 ggctcggcga aagcgccaag ggagaagcgc aaaagccatg atattggggc tcagcgagag
     1201 ggcgaggggc ggagcgcggc ggctcgccgc tgcggcggtt ctgcttgcgc cctttctcat
     1261 cttgggctgc gccaatctcg acaacagcaa atcggtggcc agcctcgtgc cgagcgccgc
     1321 gccgcgcacg accggcggcg ccgcggcggc ggcggcggcc gagcgtcgca agctcgtcga
     1381 tctcttcggc ggcgaataca gcgcgccggt cgccgagcgc tatctcaacg gcgtgctcac
     1441 ccggctcgcc gcggcgagcc cgtcgccgag cgcgccctat cgcgtcacga ttttgaattc
     1501 gccgatcgtc aacgccttcg ccttgccttc cggcgatatt ttcgtgaccc gcggcctgct
     1561 cgcgctcgcc aacgacacgt cggagatcgc cgcggtgatg gcgcatgaga tcgcccacat
     1621 caccgcgcgc cacgccgcca agcgcgccga gctggtcaaa accgcggccc tgttctcgcg
     1681 cgtctcgacc gacgtgctcc gccggccgca agaggggcag gaagaggagg cgaaagccaa
     1741 gctgtcgctc gcccatttct cgcgcgagca ggaattcgaa gccgacaaga tcggcatcaa
     1801 ggacaccgcc gcggccggct atgatcccta tgcggcctcg cgctttctcg cttcgctcgg
     1861 ccgctcgacg gctctgcgcg ccgccctgct cggcgaggcg accagcgcca agcccgacat
     1921 catgtcgacc catccctcga ccccggagcg cgtcgaggcc gctttgcagg aagcgcgcgc
     1981 gatcggcccg ccggggacgg gcgattcggg ccgcgacgcc tatctttccg cgatcgacgg
     2041 cgtcgtcttc ggcgacgatt cgacgcaagg cctggtccgc ggcacccgct tcatccatcc
     2101 caagcttggc ttcgccttca atgcgccgga cggcttcgtg ctcgacaatc agtcggcggc
     2161 tctgatcggc gtcggcgatt cgggcgccca ggctttgcgg ctcgacagca tcatggtcga
     2221 ccccgcgacc ggcctcgata cggcgatcgc ctcgggctgg atcgacggcg tcaagacctc
     2281 gtcggtccag aagacgacgg tcaacgatct gcccgccgcg acggcgatcg cgcaaggcga
     2341 tcaatggagc ttccggctcg gcgcggtgcg gatcggcgac aaggtctatc ggctgatctt
     2401 cgccgcccac gctttgacgc cgtcggtcga cgaacgcttt cgcgcctcgc tgtcgtcctt
     2461 tcatcgcctg aacctgcagg aggcctccac gattcatccg gctcacatcg agatcgtgac
     2521 ggcgcaggcc ggcgacactg ccgattcgat tgcggcgcgc atggcggtgt cggaccggcc
     2581 gctggagcat ttcctgctgc tcaacggctt ggagaagggc ggtccgctgc agtcggggca
     2641 gcgctacaag atcgtcgtcg agtgagcgcg cggagaagct cgcgccgaat tcttggattc
     2701 ggcgcgttct tcgtcgatga cccgtcgcgt ttcgccccgg atcgcttttg caaaaatggc
     2761 ccatcgaaaa atctcagggc ggctcgtcgc cgccacccat aacagcggca agctcagaga
     2821 attgcgggac ctgctgggtc cgtattcggt cgaggtcgtc agcgccggcg cgctcggcct
     2881 gccggagccg gatgagaccg gcgagaattt cgtcgccaac gccgagatca aggcgatggc
     2941 ggcggcgcga gccgccgggc tgccggcgct cgccgacgat tccgggttgt gcgtcgacgc
     3001 gctcgacggc gcgccgggga tttactcggc gcgttgggcc ggcggcggca aggatttttc
     3061 cggcgccatg cggcgtgtcg aggacgagct gagaaagaag aacgcgccgc cgccctggcg
     3121 ggcttatttc gtcagcgcgc tggcgctggc ctggccggac gatcacatcg aacgattcga
     3181 aggccgcgtc gacggcgccc tcgtctttcc gccgcgcggc gcccatggtt tcggctacga
     3241 tccgattttc gtaccggacg gccaccgatt gagctttggc gaaatgaccg ccgaggcgaa
     3301 gcacggcctt cccgccgacg gttcgcgcgc tttgtcgcat cgcgcgcggg cgttccagga
     3361 tctggcgcgc gcctgcctcg accgcgccgc ggcccgatag ggctgcgacc tctccgccaa
     3421 acgtcccgta acgattttct cgagcaaacg cgaaacgcga agccgagcgc aaccagctct
     3481 gaacgcttcc gcgccagcaa ccagcatcgg gagagaaaga tggccagaac ggtctggacc
     3541 ggcgtcgtct cgtggtccgt tgcggcggct atggcctttg cgatcgtcgc gcccgagccg
     3601 gcggcggcgc gatttttcgg cggcggcttt gcgcacagca tgcggcctgg cggcggcatg
     3661 gaaagttttc gcccgggttt tggccaaggc gatcaaagaa gcttctatcc cggcggttct
     3721 cggtttgcgg caggatgcgg atccggattt gcgccgtcaa cgcgttgcgg cggtggggag
     3781 ggagccaatc agggtcccca tcgaatcggc ttcgggccgc atgagggcgg atttgggcca
     3841 ggacctttcg ccggcggatc gattgatcct gagggacctc cggacggcga cgatccgggt
     3901 ccgatcgtcg cggccgccca ccgctcggga tcgcatgcgg gccttgccgg cggcgaacgg
     3961 ctggtcaggc agttttcgga agcggcccgg ttggcgctcg gtcgatgcgt ggccgtcgct
     4021 tggccggcga atggagcgcc ggtcgacgag accgacgcca ggcaattgcg ctgcgtcggc
     4081 gacgccttgg ccgtctatgc cgatagactt gcggacgtcg cgccgatcct tcccgaaggg
     4141 atgcggacga tacccgcggc ggtgaaggcg gcggcgagaa gcgtccgcgt tgcgaagacc
     4201 aagaccgagg ccgccgccgc gatcgcgacg gcgaccgcag tggttcacaa ggcgatcacg
     4261 ctgatcaagg ccgacgatcc gatcgtaagg tcgatcgcca cgcgagaagc cggggagatg
     4321 accaagacgc tcgatctcgc tgatgcgaaa ctcgagaagg cggtcggctt gtgaggccga
     4381 ccgacaatgc ccaagcgcgc cgcgtctaag gctccagcga cttgccgagc gcgt
//
LOCUS       DATGJW010000218         5800 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_871268, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000218 DATGJW010000000
VERSION     DATGJW010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5800)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5800)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5800
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_871268"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..2475)
                     /locus_tag="VKU03_06920"
     CDS             complement(61..2475)
                     /locus_tag="VKU03_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408956.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 1A"
                     /protein_id="HLJ71030.1"
                     /translation="MRRFARLIGFLFATAAIIFVIAAAGVGAVIWKYEQDLPDYSQLQ
                     NYEPPVMTRVHAADGSLLAEYYHERRLYLPSSAIPPLVKNAFIAAEDKNFYVHHGFDP
                     EGILRAAFVFLQGSKHVQGASTITQQVAKNFLLSNERTFDRKIREILLSMRIEQAYSK
                     DKILELYLNEIYLGLSNYGVAAAALNYFNKSVNELTIAEVAYLAALPKEPSALHPFRN
                     HERAVDRRNYVIGRMEEDGYITAEQARKARAEPLVVSPRVLTPNAIAAGYFAEEVRRE
                     LLERYGEQKLYEGGLSVRTTLDPKLQLMARKALVDGLVHYDEAHGWHGVVKTIDLGQD
                     WGLALADIPQLGDVKPWRLAVVLDANDISARIGLQPERDRAGEVGPERETGSITIEGS
                     KWTGRRPNKLVKPGDVVYVEPIAGKTGEYRLRQIPEVSGACVAMDPFTGRVLAMVGGF
                     SFDQSEFNRATQAMRQPGSSFKPFVYAAAIDNGYTPSSIILDAPIEIDQGNGEIWRPE
                     NFEGKSEGPHTLRFGIEHSINQMTVRLARDIGMPLIVEYAKKFGIYDDMPPLLAMSLG
                     AGETTVLRMVTGYSMFDNGGKRIKPTLIDRIQDRWGHTIYRHDDRICQGCDADKWDNQ
                     DEPKLIDKREQVIDPLTAYQITHIMEGVIKRGTGIAISELHRTLAGKTGTTNEAKDLW
                     FVGYSPDLAFGVFMGYDHPRSLGGSAQAALYTAPIFREFMRMALKDKPDTPFRVPPGI
                     KLVSVDVHTGMRSTGPGSILEAFKPGTAPPDAYVAGGEDQANARVLTVSPDVEKAIGS
                     GTGGLY"
     gene            complement(2601..3953)
                     /locus_tag="VKU03_06925"
     CDS             complement(2601..3953)
                     /locus_tag="VKU03_06925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylmuramoyl-L-alanine amidase"
                     /protein_id="HLJ71031.1"
                     /translation="MIHERRARDAMEALKRRAVRLIGVGFAVALALVGLTPLPAAATE
                     QSAVPVAVAARLVEYAGGARLTFDLSAPVEGRAFALADPDRIVIDVPEVNFQIDPAVG
                     RPQQGHAALGALIKSFRFGLLGPGKSRIVIDLNGPARVERLSTVAIAKGAQAARLQVD
                     LGHCEPAVFMQTVKEAEQAPPAASAAPPNEAAAPAAGPPVIVLDPGHGGIDGGAYGAG
                     VIEKTVVYDFSEELRQKLEAEHRYKVVMTRNGDEFVSLADRVRIAREANAALFISIHA
                     DSLPETADVSGATVYTLADRASDAEAARVAEHENAADKEAGLQQTPDDPGVADILFDL
                     KRRESRTYAHMFSRGVVGEWRSAGRLNRNPERSAGFVVLKAPDFPSVLLELGYLSNRQ
                     DVKALTSPEWRAKATSALVAAIDHFFAAQTGPEPEAVAGAPAAPAIAHAPQADDKSGH
                     "
     gene            4594..>5800
                     /locus_tag="VKU03_06930"
     CDS             4594..>5800
                     /locus_tag="VKU03_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HLJ71032.1"
                     /translation="MANKMIIDASHPEETRVVVLRGNRVEEFDFESANKKPLRGNIYL
                     AKVTRVEPSLQAAFVDYGGNRHGFLAFSEIHPDYYQIPVADRQALLEEEERAHGEEEN
                     EENRSDRRSRRHRRPRRHEAELRAQGDERVTEPAELDSQSSSAASEEPASTGEPNFDQ
                     PTTSEAEAAFGDVAPVAQEASEPKAPSFAEPEPAETTGRAGRAESEAAAAGEIGVEET
                     PGETEAHASESEAREDAPDHPLHDETPHDEETAEADNHDDDRSEVTTLDGGEEIAPEA
                     RVAHEEHEEEGEPVEQVGGDAMEELPRRPPRLRRQYKIQEVIKRRQVLLVQVVKEERG
                     NKGAALTTYLSLAGRYSVLMPNTARGGGISRKITNSADRSRLKSIAQDLDVPEGMGVI
                     LRTAGAMRTK"
BASE COUNT         1019 a         1885 c         1932 g          964 t
ORIGIN      
        1 agtgggcaat cggcaggcga tgcgcgcccg accgccgact gcctaatgcc gattgccgcg
       61 ttaataaagc ccgcccgttc ccgagccgat cgccttctcc acatcgggcg agacggtcag
      121 cactcgggcg ttggcctggt cctcgccgcc cgcgacatag gcgtcgggcg gcgcggtgcc
      181 gggcttgaag gcttcgagga tcgagcccgg cccggtcgag cgcatgccgg tatggacatc
      241 gacggaaacc agcttgatgc ccggcggcac gcggaacggc gtatccggct tgtccttcag
      301 cgccatgcgc atgaattcgc ggaagatcgg cgccgtatag agcgccgcct gggccgagcc
      361 gccgagcgag cgcggatggt cgtagcccat gaacacgccg aaagcgaggt ccggcgaata
      421 gccgacgaac cacaaatcct tcgcctcgtt ggtcgtgccg gtcttgccgg cgagggtgcg
      481 gtgaagctcg gaaatggcga tgccggtgcc gcgcttgatc acgccttcca tgatgtgcgt
      541 gatctggtac gcggtcagcg gatcgatcac ttgctcgcgc ttgtcgatca gcttcggctc
      601 gtcctgattg tcccatttgt cggcgtcgca gccctggcag atgcggtcgt cgtgccgata
      661 gatggtatgg ccccagcggt cctggatgcg gtcgatcagg gtcggcttga tccgcttgcc
      721 gccattgtcg aacatcgaat agccggtgac catgcgcagc acggtcgtct cgccggcccc
      781 gagcgacatg gcgaggagcg gcggcatatc gtcataaatg ccgaacttct tggcgtattc
      841 gacgatcagc ggcatgccga tgtcgcgggc gaggcgcacc gtcatctggt tgatcgaatg
      901 ctcgatgccg aaacgcagcg tgtgcgggcc ttccgacttg ccctcgaaat tttccgggcg
      961 ccagatttcg ccattgcctt gatcgatctc gatcggcgcg tcgagaatga tcgacgacgg
     1021 cgtatagccg ttatcgatcg ccgcggcgta gacgaagggc ttgaacgacg agcctggctg
     1081 gcgcatcgcc tgggtcgcgc ggttgaattc cgactgatcg aaggagaagc cgccgaccat
     1141 ggcgaggacg cggccggtaa acgggtccat cgcgacgcag gcgccggaga cttcgggtat
     1201 ctggcgcagc cggtattcgc cggtcttgcc ggcgatcggc tcgacataga cgacgtcgcc
     1261 cggcttcacc agcttgttcg gccgccttcc ggtccatttc gacccttcga tcgtgatcga
     1321 tccggtctcg cgctcggggc cgacctcgcc ggccctgtcg cgctcaggct gcaagcctat
     1381 ccgcgccgaa atgtcgttgg cgtcgaggac caccgccagc cgccagggct tgacgtcgcc
     1441 gagttgcgga atgtcggcga gcgcaaggcc ccaatcctgg ccgagatcga tcgtcttgac
     1501 gacgccatgc cagccatgcg cctcgtcgta atggacgagg ccgtcgacca gagccttgcg
     1561 cgccatcaac tgcaatttcg gatcgagcgt ggtgcggacc gagaggccgc cttcatagag
     1621 cttctgctcg ccgtagcgct cgagcagctc gcggcgcact tcctcggcga aatagccggc
     1681 ggcgatcgcg tttggcgtca gcacgcgcgg actgacgacg agcggctcgg cgcgcgcttt
     1741 gcgcgcctgc tcggcggtga tgtagccgtc ctcctccatg cggccgatca cgtagttgcg
     1801 ccggtcgacg gcgcgctcgt gattgcggaa cggatgcagc gcgctcggct ccttgggcag
     1861 agcggcgaga taggcgactt cggcgatcgt cagctcgttg accgacttgt tgaagtagtt
     1921 gagcgccgcc gccgccacgc cgtaattcga caggccgaga tagatttcgt tgagatagag
     1981 ttcgaggatc ttgtccttcg aataggcctg ctcgatgcgc atcgacagaa ggatttcgcg
     2041 aatcttgcgg tcgaacgtgc gctcattgga caagaggaag ttcttggcca cctgctgggt
     2101 gatggtcgag gcgccctgca catgtttcga gccctgcagg aagacgaagg cggcgcgcag
     2161 gatgccttcc ggatcgaagc cgtgatggac gtagaaattc ttgtcctcgg cggcgatgaa
     2221 cgcgttcttg accagcggcg ggatggcgct cgacggcaga tagagacgcc gctcgtgata
     2281 gtattcggcg agcaggctgc cgtcggcggc gtgaacgcgc gtcatgaccg gcggttcgta
     2341 attctgcaat tgcgagtagt cgggcaggtc ctgctcgtat ttccagatca ctgcgccaac
     2401 gccggcggcg gcgatgacga aaatgatcgc cgcggtcgcg aacaggaagc cgatcagccg
     2461 ggcgaaccgc cttatcatcc caaaatcaac ccttccccgc cggccttcgc ttgcgactca
     2521 aaatcggctc aacccatcaa acgatcgatt tgtgttgcga tcgcggcaag ctcgccaggc
     2581 gcctttcgta aggcgcgctt tcaatggccg ctcttgtcgt cggcctgcgg cgcatgagca
     2641 atggcggggg cggcgggcgc gcccgcaacc gcttccggct ctggcccggt ttgcgcggcg
     2701 aagaaatggt cgatcgccgc aaccagcgcg ctcgtcgcct tagcccgcca ttccggcgag
     2761 gtcaacgctt tcacgtcctg gcggttggat aggtagccaa gctccagaag aaccgaagga
     2821 aaatccggcg ctttgagcac cacgaagccg gccgagcgct cgggattgcg gttgaggcgg
     2881 ccggccgagc gccattcgcc gaccacgccg cgcgagaaca tatgggcgta ggtgcgggat
     2941 tcgcggcgct tcagatcgaa aagaatgtcg gcgacgccgg gatcgtcggg cgtttgttgg
     3001 agcccggcct ccttgtcggc ggcgttctca tgctcggcga cgcgggcggc ctcggcgtcg
     3061 gaggcgcggt cggcgagcgt atagacggtc gcgcccgaga cgtccgcggt ttcgggcagg
     3121 ctgtcggcgt ggatcgagat gaacaaggcg gcgttggcct cgcgcgcgat gcgcacgcga
     3181 tcggcgagcg agacgaattc gtcgccattg cgggtcatca ccactttata gcgatgctcc
     3241 gcttcgagct tttgccgcag ttcctcggaa aaatcataga cgacggtttt ctcgatcacg
     3301 ccggcgccgt aggcgccgcc gtcaatgccg ccatgaccgg gatcgagcac gatgaccggc
     3361 ggcccggccg ccggcgcggc cgcctcgttg ggcggcgccg cgctggccgc aggcggcgcc
     3421 tgctcggcct ctttcacggt ctgcatgaag accgccggct cgcaatggcc gagatcgacc
     3481 tgcaggcgcg ccgcctgggc gcccttggcg atcgccaccg tcgacagccg ctcgacccga
     3541 gccgggccgt tgagatcgat gacgatccgc gatttgcccg gcccgagcag cccgaaccgg
     3601 aaggatttga tgagcgcgcc caacgccgca tggccctgct gcggccggcc gaccgccgga
     3661 tcgatctgaa aattgacttc cggaacgtcg atgacgatgc ggtccggatc ggccaaggcg
     3721 aaagcgcggc cttcgaccgg cgccgaaaga tcgaaagtca gccgcgcgcc gcccgcatat
     3781 tcgacaagcc gggccgcgac cgcgacggga acggccgact gttcggtggc ggcggccggc
     3841 aatggagtaa ggccgacaag agcgagggcg accgcaaagc cgacgccgat caggcgcacg
     3901 gctcttcgtt tcaacgcctc catcgcgtcg cgcgcgcgcc tttcgtgaat cattctcgat
     3961 cgaccaaagt cggcggcgtc gccgccggcg ctcgatccct aagccataag gcggcgaggg
     4021 ttaacgagca ataaccaggc cggcgcgcaa tcgttcgttc tgttgcagcg ccgccacagc
     4081 ggcggaattg ccttgccaag cttgccggtc ttccatatgt agatcgctgc ttcgatgcgg
     4141 ctcgaatccg tttacgacgc cctcctggcc ttttgtcggc gctcaggaat cggcgacggc
     4201 gttccggatc cagcccgagc gggactgtaa accggcggac gcgttgtctg tcgccttaag
     4261 cgcctctcct ttttggagtt ttgcgctttg gacccgcgaa gcgacggcgc ttttgacggc
     4321 gcccctcgat tggcgacgag gataaggctc cgccagtgaa ctggtgcgca atgcccgtcc
     4381 gatccctgga aagccccgct cccgcgcttc gcaaggcgcg cgacggatcg ctttcccgcg
     4441 gcgcgggcca gcgcgacgcc gcccgccggg ccggcgccgc cctatcccat cgctttccct
     4501 catcccgatc caacagaggc cgccttttga gcgccggcag acgcttcccc gaagcgtcgc
     4561 cgacggcgcc cgcgcggccg caaagagttc aacatggcca acaaaatgat catcgacgca
     4621 tcccacccgg aagagacccg ggttgtggtg cttcgcggca atcgcgtcga ggaattcgac
     4681 ttcgaatccg ccaataagaa gccgctgcgc ggcaacatct atctcgccaa agtcacccgc
     4741 gtcgaaccgt ccctgcaagc cgccttcgtc gattacggcg gcaatcgcca cggcttcctc
     4801 gccttctcgg aaatccatcc cgactattac cagatccccg tcgccgaccg gcaggcgctg
     4861 ctcgaagagg aggagcgcgc ccatggcgag gaggagaacg aggagaatcg cagcgaccgg
     4921 cgcagccggc gtcatcgccg gccgcgccgc cacgaagcgg aattgcgcgc gcagggcgac
     4981 gagcgcgtca cggagccggc cgaactggac agtcagtcct caagcgccgc gagcgaagag
     5041 ccggcgtcga ctggcgagcc gaatttcgat cagccgacca cgtcggaagc cgaagcggcg
     5101 ttcggcgacg tcgcgccggt cgctcaggag gcgagcgagc ccaaggcgcc atccttcgcc
     5161 gaacccgagc cggccgaaac caccggccgt gccggccgcg ccgagtccga agctgcggcg
     5221 gcgggcgaga tcggcgtcga ggagacgccg ggcgagactg aggctcatgc cagcgagagc
     5281 gaagcgcgcg aggatgcgcc cgatcatccg ctccacgacg agacgccgca tgacgaagag
     5341 acggcggaag cggacaatca cgacgacgac cgcagcgagg tgacgacgct cgacggcggc
     5401 gaggaaatcg cgccggaggc ccgcgtcgct cacgaagagc acgaagagga gggcgagccg
     5461 gtcgagcagg tcggcggcga cgcgatggag gaattgcccc gccgcccgcc gcgcctgcgc
     5521 cgacaataca agattcaaga agtgatcaag cgtcgccagg tgctgctcgt ccaggtcgtc
     5581 aaggaggagc gcggcaataa gggcgcggct ttgaccacgt acctgtcgct ggccggccgc
     5641 tattccgtgc tgatgccgaa tacggcgcgc ggcggcggca tttcgcgcaa gatcaccaac
     5701 agcgccgacc gcagccggct gaaatcgatc gcccaggacc tcgacgtgcc ggaaggaatg
     5761 ggcgtgatcc tgcgcaccgc cggcgcgatg cgcaccaagg
//
LOCUS       DATGJW010000219         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1389466, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000219 DATGJW010000000
VERSION     DATGJW010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389466"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VKU03_06935"
     CDS             <1..333
                     /locus_tag="VKU03_06935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186942.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-formylglutamate amidohydrolase"
                     /protein_id="HLJ71033.1"
                     /translation="AAPDFVIGDRYGASAALWVVETLEQALRKLGYSVRRNKPYAGGF
                     ITEHYGAPASRRHAVQIEINRALYMDERTVAKSERWTDLAVNLQAVVETLIVRIGREI
                     ARPPLAAE"
     gene            406..576
                     /locus_tag="VKU03_06940"
     CDS             406..576
                     /locus_tag="VKU03_06940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71034.1"
                     /translation="MRQVMPHRNNIELRRTKIKRYFSIRWKNCSDSLETLSARPRAQT
                     WSEREFSARTGI"
     gene            657..1460
                     /gene="hisN"
                     /locus_tag="VKU03_06945"
     CDS             657..1460
                     /gene="hisN"
                     /locus_tag="VKU03_06945"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HLJ71035.1"
                     /translation="MTAIDFSGFVDRLAQASAEVILPFFRSAIGAEDKSRGGAFDPVT
                     EADRAAEVAMRRLIAQTFPAHGIIGEEYGVERPDAEYVWVLDPIDGTKAFISGLPTWG
                     TLIGLTHRGRPVYGMMSQPFTRERYFGDGVSAGWRGLAASHNDSPASRPLHVRACASL
                     AEATLMTTAPQMFHGAEAPAFQRVEERARLCRYGADCYGYCALAAGFVDIVVEAGLKP
                     HDIVALVPIVEGAGGVVTTWDGGDPSGGGRIVAAGDTRVHQEALQLLAL"
     gene            complement(1463..>2344)
                     /locus_tag="VKU03_06950"
     CDS             complement(1463..>2344)
                     /locus_tag="VKU03_06950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71036.1"
                     /translation="AAAACGMIGLVLFLPIGWRIAADSGAGAPLLGGGAEIAASLARA
                     AARLSAAPPARSGFDDVAVALGAAILPPLATSAFASAAASSARRAGLFGVAAAALFAL
                     AFAVGAPLWQAYQGVAGQSLRASALLISAIVCAAAGVHTASRATSAESAYGYENVLAS
                     ARLARSRGLALLAVGIGALCALNHAVRPAEALVGALALSLAFLGPPLALAASSRSRAA
                     HAIASVATSLATIAALAWTNGLAFVLAQAPAVALTGAAAGFALGWAASLFGGSPRGAA
                     EPPPAGLFVESPFDPDA"
BASE COUNT          394 a          787 c          848 g          315 t
ORIGIN      
        1 gcggcgcccg atttcgtgat cggcgaccgc tacggggcca gcgccgcgct ttgggtggtc
       61 gagacgctgg agcaggcttt gcgcaagctc ggctattcgg tccgccgcaa caagccgtac
      121 gccggcggct tcatcaccga gcattacggc gcgcccgcca gccgccgcca tgcggtccag
      181 atcgagatca accgggcgct ttacatggac gagcgaacgg tggcgaaatc ggagcgctgg
      241 acggatctcg ccgtcaatct gcaggccgtg gtcgagacgc tgatcgtccg catcggccgg
      301 gaaatcgccc ggccgcccct cgcggcggag tgaggacaaa aaaggaggcc gcctgtgtcg
      361 ccacaagcgg cccaagtcta gggaggaaac gcccaaggag ggcatatgcg gcaggtcatg
      421 ccgcaccgca acaatatcga actgcgccgc accaaaatca agcgctattt ttccatccgg
      481 tggaaaaact gctcggattc cctggagacg ctgtcggcaa ggccgagggc gcaaacatgg
      541 tccgaacggg aattttccgc gagaacaggg atttaaatgt ttatcttgat atatcgccgc
      601 tgcgttcaaa tgaagcagga gtataggaga ggcgccgctt tcgcaggggc gggcggatga
      661 ccgcgattga tttttcaggc tttgtcgatc gcctcgcgca agcctcggcc gaggtgattc
      721 tgcctttctt tcgcagcgcg atcggcgccg aggacaaatc gcgcggcggc gccttcgatc
      781 cggtcaccga ggccgaccgg gcggcggaag tcgccatgcg ccggctcatc gcgcaaacct
      841 tcccggcgca tggcattatc ggcgaggaat atggcgtcga gcggcccgac gccgaatatg
      901 tctgggtcct cgacccgatc gatggcacca aggccttcat ctccggcctg ccgacctggg
      961 gcactttgat cggcctgacc catcgcgggc ggccggtcta tgggatgatg tcgcagccct
     1021 tcacgcgcga gcgctatttc ggcgacgggg tcagcgccgg ctggcgcggt ctcgccgcga
     1081 gccacaacga ctcgccggcg agccgcccgc tccatgtccg cgcctgcgct tccctggccg
     1141 aggcgacgct gatgacgacc gcgccgcaaa tgttccatgg ggcggaggcg ccggcgtttc
     1201 agcgggtcga ggagagggcg cgcctctgtc gctacggcgc cgattgctac ggctattgcg
     1261 cgctggccgc cggcttcgtc gacatcgtcg tcgaggccgg cctgaagccg cacgacatcg
     1321 tcgccctggt gcccatcgtc gagggcgccg gcggcgtggt cacgacctgg gatggcggcg
     1381 atccttcggg cggcgggcgc atcgtcgccg cgggcgatac gcgcgtccat caagaggcgc
     1441 tgcagctcct cgccctatga gcttaagcgt ccggatcgaa cggcgattcg acgaacaggc
     1501 ctgccggcgg cggctcggca gcgccgcgcg gcgagccgcc gaaaagactc gccgcccagc
     1561 ccagcgcgaa gcccgccgcc gcgccggtca gcgcgaccgc cggcgcctgc gcgaggacga
     1621 acgccaagcc attggtccag gccagcgcgg cgatcgtcgc caggctggtt gcgaccgaag
     1681 cgattgcatg cgccgcgcga ctgcgggagg aagcggcaag agcgagcggc gggccgagaa
     1741 aggcgagcga cagcgccagc gcgccgacca gcgcctcggc cggccggacg gcgtgattga
     1801 gggcgcataa ggcgccgatg ccgaccgcga gaagcgccag gccgcgcgag cgggcgaggc
     1861 gcgcgctggc gagcacgttc tcgtagccat aggcggattc ggcgctggtc gcgcgcgagg
     1921 cggtgtgcac gcccgccgcc gcgcaaacga tggcgctgat cagcaacgcg ctggcgcgca
     1981 ggctttggcc ggcgacgccc tggtaggcct gccaaagcgg cgcgcccacg gcgaaggcga
     2041 gcgcgaacaa agccgccgcc gcaacgccga acaggccggc ccgccttgca gacgaagccg
     2101 cggcgctggc gaaggcgctg gtcgcgagcg gcggcaggat ggccgcgccg agcgcgacgg
     2161 cgacatcgtc gaagccggag cgggcgggcg gggcggcgct cagccgcgcc gcggcgcggg
     2221 cgagactggc ggcgatttcg gcgccgccgc cgaggagcgg cgcgccggcg ccggaatcgg
     2281 cggcgatgcg ccacccgatc ggcaggaaaa ggaccagccc gatcatcccg caagcggcgg
     2341 ccgc
//
LOCUS       DATGJW010000220         3437 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_41208, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000220 DATGJW010000000
VERSION     DATGJW010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3437)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3437)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3437
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_41208"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(384..2498)
                     /locus_tag="VKU03_06955"
     CDS             complement(384..2498)
                     /locus_tag="VKU03_06955"
                     /inference="COORDINATES: protein motif:HMM:NF024215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FUSC family membrane protein"
                     /protein_id="HLJ71037.1"
                     /translation="MIRAWPRSPAVLKLLVGLHIVNGLSVAAGVAVVSVLASLLFGFA
                     AGQPATLGAIGASISDFPAPLRNKAATMAVGFGLALCATAIVLLLESHAALLLAAIAA
                     IAFIAGFVSGFGRWALALSMQILIPMVFALSLPSTDFAGALRNEALLAGGGLAYIAVA
                     LLLTAWTDAGGRRLMTSEALREFSAYLRAVSGFYEQGADLQDTYGAVIRQQAALADQL
                     QAARALLLDQPMRTKARIRLAASIGILLDAFDQLVAAHVELGEIRRAPACKTLMARIR
                     VTLLASAIDLDRLSLDLLSNAAPRLPPDHSLASDALLREAERLAKDESLDEAAHAAAK
                     ATSERLIAALAHIRRLERALADDEEARASMRGLDLAAFAPRPSFDLKLLKPHLNPDSP
                     VFRFSARLALAMTAGGLVAESLSGVRHGNWVLLTIAVIMRASYGLTRQRRNDRVIGTL
                     IGCLLAAGLIAVAPLGVLVAAQAIALGVTHGFARLRYLVTATGASVLALLSVHLVDPS
                     EASPVVARLADTLIGAAIAHLFSHILPRWEYDEARRLAARLQADLVVFAKTALDLKAS
                     GQDYRLARKRIIESLAALSDSAGRMGGEPQAVRRGLSELADMLIAGYVLAANVSAARF
                     MARDKRGAADYELIVRHLDATRQWLTGFLSGPRSAPDLEGPALVTGPPNEALGGEFLR
                     LRKAALALLAASAAYRRAAAEA"
     gene            complement(2716..3216)
                     /locus_tag="VKU03_06960"
     CDS             complement(2716..3216)
                     /locus_tag="VKU03_06960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71038.1"
                     /translation="MLRTLATTFALAAGAALPLANTAEAAMTVDSLGKALAQANPVEK
                     TQFFWNGYNYCWYDNGWRGPGWYWCGYAWRYGFGWGGGFGWHGWRWHGWHRGWHGGHW
                     PGGHWHGGNWHNGGNWNGARTYQGHAGGPPMHMHMGGNHMGAHMGGMHMQGGAHGGHG
                     GGQRPH"
BASE COUNT          593 a         1157 c         1198 g          489 t
ORIGIN      
        1 cccgatcttg aacgatctgg agggcgaacg cggcgacgcc gctcggcggc gcgggctcgc
       61 atcgtaatcg gttcgagcgc ccgggcgagc ctgtaggccg gtcgctgacc gcgcctttcc
      121 ctcccttcgc tacggcatgc acacatcttg aagcgcgggc gtgtctgagc cgtgctcgcc
      181 gtcaaggcca agcgcgcttc gcgcgtcgct cgccaaagca ggctcaggag aaaatcagtt
      241 ggcgcggcgc gatggctcgc gagccttgac ggctccgcgc ggctcaggct ccgcgccgag
      301 atgcgtgcat gccgtagcgc ctatagaggg aaattgtttt cccggatcga gaaaattggc
      361 cgatctttgg ccgaacttcg ctctcacgct tccgccgccg cccgccgata ggcggcggaa
      421 gcggccagca gcgccagcgc cgctttgcgc aggcgcagga attccccgcc cagcgcctcg
      481 ttgggcgggc cggtcaccag agccgggcct tcgaggtcgg gcgccgaacg ggggccggaa
      541 agaaagccgg tcagccattg ccgggtcgcg tcgagatggc ggacgataag ctcgtagtcc
      601 gccgcgcctc tcttgtcgcg cgccatgaac cgcgccgccg aaacattggc ggcgaggaca
      661 tagccggcga tcagcatgtc cgcgagctcg ctcaggccgc gccgcaccgc ctgcggctcg
      721 ccgcccatcc ggccggccga atcggagagc gccgcgagcg attcgatgat gcgcttgcgc
      781 gccaggcgat agtcctggcc ggacgccttc aggtcgagcg ccgtcttggc gaagacgacg
      841 aggtcggcct gcagccgcgc ggcgaggcgc cgcgcctcgt cgtactccca gcgcggcagg
      901 atgtgactga agagatgggc gatcgccgcg ccgatcagcg tgtcggcgag gcgggcgacg
      961 acgggcgagg cttcgcttgg atcgacgaga tggaccgaga gcagcgccag caccgaggcg
     1021 ccggtcgccg tcaccagata gcgcagccgg gcgaagccgt gggtcacgcc gagcgcgatc
     1081 gcctgcgccg cgacgagaac gccgagcggc gcgacggcga tcaggccggc cgcgagcagg
     1141 cagccgatca gcgtgccgat cacccggtcg ttgcgccgct ggcgcgtcag accgtagctc
     1201 gcgcgcatga tgacggcgat ggtcagcagg acccaattgc cgtgccgcac cccgctcaag
     1261 gactccgcca ccagcccgcc ggcggtcatc gccaaagcca gccgcgcgga aaagcgaaag
     1321 accggcgaat cgggattgag atgcggcttc agcaatttga ggtcgaaaga ggggcgcggc
     1381 gcgaaggcgg caaggtcgag gccccgcatc gaggcgcgcg cctcctcgtc gtcagcgagc
     1441 gcccgctcca ggcggcggat atgggcgagc gcggcgatca gccgctcgct cgtcgccttg
     1501 gcggcggcat gcgccgcttc gtccaggctt tcgtctttcg ccagccgctc ggcttcgcgc
     1561 agcagcgcgt cggaggcgag cgaatgatcc ggcggcaggc gcggcgcggc gttcgagagg
     1621 aggtcgaggc tgaggcgatc aaggtcgatg gcgcttgcga gcaaggtgac gcgaatgcgc
     1681 gccatcagcg tcttgcaggc cggggcgcgg cggatttcgc cgagctcgac atgcgccgcg
     1741 acgagttgat cgaaggcgtc gagcagaatg ccgatggaag cggccagcct gatccgcgcc
     1801 ttggtccgca tcggctggtc gagcagcagc gcgcgcgccg cctgcaactg gtcggcgagc
     1861 gccgcctgct ggcgaatgac ggcgccgtac gtatcctgga gatcggcgcc ttgctcgtaa
     1921 aagccggaaa cggcgcgcag ataggccgag aattcccgca aggcttcgct ggtcatcaag
     1981 cgccggccgc cggcgtcggt ccatgcggtc agcagcagcg cgacggcgat ataagcaagg
     2041 ccgccgccgg ccagcagcgc ttcgttgcgc aaggcgccgg cgaaatccgt cgacggcagg
     2101 ctcagcgcga agaccatcgg aatcaggatt tgcatgctca aggccagcgc ccagcgccca
     2161 aagcccgaga cgaagccggc gatgaaggcg atggcggcga tggcggcgag caacagggcg
     2221 gcatggcttt cgaggaggag gacgatcgcc gtcgcgcaaa gggcgaggcc gaagccgacc
     2281 gccatcgtcg cggccttgtt gcgcaaaggc gcgggaaagt cgctgatgct cgcgccgatc
     2341 gcgccgagcg tcgccggctg gccggcggcg aaaccgaaaa ggagcgaggc gaggacggag
     2401 acgacagcga cgcccgccgc cacgctgagg ccgttgacga tatgcaggcc gaccagcaat
     2461 ttgagcacag ccggcgagcg gggccaggcg cggatcatgg cgcaggcgcc cttcccgcgc
     2521 cgccccgcga agaatatccg ccgctatcgc gccgtccgcg cctcgacacg attctaagcc
     2581 tcagcccgca ctctcgcccg ctttcctatc agggcggagg atggctgcgc aaaaagcccg
     2641 gtccgcattt caaaaaaaat cgccttcgcc cggcgacggg cgaaggcgtt ttgtcaaacg
     2701 agacgcgttg ccggcttaat gcggccgctg gccgccgcca tgaccgccat gcgcgccgcc
     2761 ctgcatgtgc atgccgccca tatgcgctcc catatggttg ccgcccatat gcatatgcat
     2821 cggcggcccg cccgcatggc cttggtacgt gcgggctcca ttccaattgc cgccgttatg
     2881 ccagttgccg ccatgccaat ggccgcccgg ccaatggccg ccatgccagc ctcgatgcca
     2941 gccatgccag cgccagccgt gccagccgaa gccgccgccc cagccgaagc cgtagcgcca
     3001 ggcatagccg caccagtacc agcccggccc gcgccagcca ttgtcgtacc aacaataatt
     3061 gtagccgttc cagaagaact gcgtcttctc gaccggattg gcctgcgcca gcgccttgcc
     3121 gagcgaatcg accgtcatcg ccgcctcggc cgtgttggcg aggggcagcg cggcgccggc
     3181 ggcgagcgcg aatgtggtgg cgagtgtgcg aagcatgttc catcctctca tgacgaaaaa
     3241 cgccgctttg gcgggttaaa cgccgagaga aggggaaaag ttcgaaccgg cgggaggaat
     3301 gccgccgaaa ttcgcgcggc gcgcctcatt gcgaggagcg cggcgacgac gccctccatg
     3361 agcggacggc cttgctccgc tcgccatgac ggcctagggt ctgttgggat tcaggattct
     3421 catcctggtt tggttgt
//
LOCUS       DATGJW010000221         3843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_248878, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000221 DATGJW010000000
VERSION     DATGJW010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3843
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_248878"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..536
                     /locus_tag="VKU03_06965"
     CDS             <1..536
                     /locus_tag="VKU03_06965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71039.1"
                     /translation="AYLHKKIAAMEREFGYDATYTHEMLDASLPAFLKFALFQSMAQH
                     RDSAPREAWYAAKLAGAMSEDCGPCSQLVVDMALKAGIDPKILAALIRGDFTRAGEHA
                     ALGFRYGQAVAANAPEALQLALDAKARYGERGLVALALAVAGARVYPTLKRGLGHGAA
                     CRKLVVADDVIAVRKAA"
     gene            533..1441
                     /gene="sigJ"
                     /locus_tag="VKU03_06970"
     CDS             533..1441
                     /gene="sigJ"
                     /locus_tag="VKU03_06970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013501062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor SigJ"
                     /protein_id="HLJ71040.1"
                     /translation="MKGDIAIFERCRPRLLNIAYRMLGEMSAAEDVVQEAWLRWSAIE
                     ASDIRDPLAWLSTATVRLALDALRAARARRESYVGPWLPEPILPDDARIFAADAPAEH
                     AELASDLSLALMHVLERLSPEERAALILHDAFDFDYDEIAATLGKSEPACRKLVSRAR
                     ERVKTERPRFSAKSEAHRDLLARFAKASAAADARELAALFAPDAIAYTDGGGRVRAAL
                     NPIYGADNVVRLIIGLASKSNFLDTLQWKSTEINGRPAMVSIAHGDPLTAITLETDGE
                     KITAIYVQRNPDKLGRVSRALGWPAG"
     gene            complement(1585..2661)
                     /locus_tag="VKU03_06975"
     CDS             complement(1585..2661)
                     /locus_tag="VKU03_06975"
                     /EC_number="5.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047472 - 3-carboxy-cis,cis-muconate
                     cycloisomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-carboxy-cis,cis-muconate cycloisomerase"
                     /protein_id="HLJ71041.1"
                     /translation="MTISAFDHPILSALVGDAEIAAAFSVEAEIRAMLAFEAALAEAE
                     ASVGLIAPEAASRIAAVCAAFSPDLAALAEGAAQDGVVVPALVTQLRREVGEAHAASL
                     HRGATSQDVIDTSLVLRLKAVVAIFGARLDALIAALRALEAEQGQTPLMGRTRMQRAR
                     PIRAADKLRAWREPLQRHRERLEALTPRLLVVQFGGAVGTRDGLDGKGEAIAAELARR
                     LGLGDGPCWHVERDSLAEFAAWLSLVSGALGKIGQDVALLAQNEIGEIKLAEGGGSSA
                     MPHKSNPVRAEILVALARFNAAMLGAQHQALIHENERSGAAWTLEWLIIPQMVVATAA
                     GLRHAAALCKGMRFVAAKNRSSPV"
     gene            complement(2840..3466)
                     /gene="pcaG"
                     /locus_tag="VKU03_06980"
     CDS             complement(2840..3466)
                     /gene="pcaG"
                     /locus_tag="VKU03_06980"
                     /EC_number="1.13.11.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002965991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018578 - protocatechuate 3,4-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0046278 - 3,4-dihydroxybenzoate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit alpha"
                     /protein_id="HLJ71042.1"
                     /translation="MVQKLDHLKETPSQTAGPYVHIGATPNFSGIEGVYAQDLGRTMV
                     GRDAKGERITIVGRVLDGLGEPLKDALVEIWQADAAGLYNSPQERRGKADPHFAGFGR
                     QATDYVTGEYRFETIKPGPVPFVDGRPMAPHVTFWIVARGINVGLHTRMYFSDEAAAN
                     AKCPVLARLEHQSRVDSLIARRSDKGGRATYHFDIRLQGENETVFFDI"
     gene            complement(3466..>3843)
                     /locus_tag="VKU03_06985"
     CDS             complement(3466..>3843)
                     /locus_tag="VKU03_06985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017266396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protocatechuate 3,4-dioxygenase subunit beta"
                     /protein_id="HLJ71043.1"
                     /translation="PLDPNFGGCGRTITDSDGRYWFRTVRPGPYPWPNRVNDWRPAHI
                     HFSIFGHAFTQRLITQMYFEGDPMIWQCPIATGIPDREALEQLVAALDRNNSTHMDSL
                     AYKFDIVLRGRRSTPFENKKEGN"
BASE COUNT          620 a         1299 c         1312 g          612 t
ORIGIN      
        1 aggcctatct gcacaagaaa atcgccgcga tggagcgcga attcggctat gatgcgacct
       61 atacgcatga aatgctcgac gcctcgctgc cggctttcct gaaattcgct ttgttccaga
      121 gcatggcgca gcatcgcgac agcgcgccgc gcgaagcgtg gtacgccgcc aagctcgccg
      181 gggcgatgag cgaagattgc ggcccctgct cgcagctcgt cgtcgacatg gcgctgaaag
      241 ccggaatcga tccgaaaatc cttgcggctt tgatccgcgg cgatttcacg cgcgccggcg
      301 agcacgccgc gctcggcttc cgctacggcc aggcggtcgc ggccaacgcg ccggaggcgc
      361 tgcagctcgc gctcgacgcc aaggcgagat atggcgaacg cggcctcgtc gcgctcgcgc
      421 tcgccgtcgc cggcgcgcgc gtctatccga cgctgaagcg cggcctcggc catggcgccg
      481 cctgccggaa actggtggtc gccgacgacg tgatcgcggt aaggaaggcg gcatgaaagg
      541 cgacatcgcg attttcgagc gctgccggcc gcgcctgctc aacatcgcct atcgcatgct
      601 cggcgaaatg tcggcggccg aggacgtcgt ccaggaggcc tggctgcgct ggagcgcgat
      661 cgaggcgagc gacatccgcg atccgctcgc ctggctgtcg accgcgacgg ttcggctcgc
      721 gctcgacgcg ttgcgcgcgg cgcgcgcccg gcgcgaatcc tatgtcggcc cctggctgcc
      781 cgagccgatc ctgccggacg atgcccgcat cttcgccgcc gacgcgccgg ccgagcatgc
      841 cgagctcgct tccgacctgt cgctggcgct gatgcatgtg ctcgagcgcc tgtcgccgga
      901 agagcgcgcc gctttgatcc tgcacgacgc gttcgatttc gactacgacg agatcgccgc
      961 aacgctcggc aagagcgagc ccgcctgccg caagctggtc agccgggcgc gcgagcgggt
     1021 caagaccgaa cggccgcgct tttcggcgaa gagcgaggcc catcgcgacc tgctcgcgcg
     1081 cttcgccaag gccagcgccg cagccgacgc gcgcgagctt gcggctttgt tcgcgcccga
     1141 cgcgatcgcc tataccgacg gcggcggccg cgtcagagcc gcgctcaatc cgatctacgg
     1201 cgctgacaat gtcgtgcgcc tcatcatcgg cctagcgtcg aaatccaact tcctcgacac
     1261 gctgcaatgg aaatcgaccg agatcaacgg ccgcccggcc atggtcagca tcgctcatgg
     1321 cgacccgctc accgcgatca cgctggagac cgacggcgag aagatcaccg cgatctacgt
     1381 acagcgcaat ccggacaagc tcggccgggt gtcgcgggcg ctcggctggc cggcgggttg
     1441 agggtacgat cctcccccgt cgaagacggg ggagggggac cacgcgaagc atggtggagg
     1501 ggccccggtc tatcaggata aatcccaacc gccgttgccc cctccaccgc ccttcgggcc
     1561 gtccccctcc cccgtttcgc ttcgctacac gggggaggat cggttcttcg ctgcgacgaa
     1621 ccgcatgccc ttgcacagcg ccgcggcgtg gcgcaatccg gcggcggtcg cgacgaccat
     1681 ttgcggtatg atgagccatt cgagcgtcca ggcggcgccg gagcgctcgt tctcatggat
     1741 cagcgcctgg tgctgcgcgc cgagcatagc ggcgttgaac cgcgccagcg cgacgaggat
     1801 ttcggcgcgc accggattgg atttatgcgg catggccgag gagccgccgc cttcggcgag
     1861 cttgatttcg ccgatctcgt tctgcgccag cagcgcgaca tcctgaccga tcttgccgag
     1921 cgcgccggag accagcgaca gccacgcggc gaattccgcc agcgagtcgc gctcgacatg
     1981 ccagcacggc ccgtcgccga gaccgagccg gcgcgccaat tcggcggcga tcgcttcgcc
     2041 tttgccgtcg agcccgtcgc gcgttccgac cgcgccgccg aattggacga cgagcaggcg
     2101 cggcgtcagc gcctccagcc tttcgcgatg gcgctgcaac ggctcgcgcc aagcgcgcag
     2161 cttgtcggcg gcgcggatcg gccgcgcccg ctgcatgcgg gttcggccca tcagcggcgt
     2221 ttggccttgc tcggcttcga gcgcgcgcaa ggcggcgatc agcgcgtcga gacgcgcgcc
     2281 gaatatcgcg acgacggctt tgaggcgcag gacgaggctg gtgtcgatga cgtcctggct
     2341 ggtcgcgccg cgatggaggc tggcagcgtg cgcctcgccg acctcccgac gaagctgcgt
     2401 gacgagcgcc gggacgacga cgccgtcctg cgctgcgccc tcggcgagcg cggcgagatc
     2461 gggcgagaaa gcggcgcaga ccgcggcgat gcgcgaggcc gcctccggcg cgatcaggcc
     2521 gaccgacgcc tcggcctcgg cgagcgccgc ctcgaacgcg agcatcgccc tgatctccgc
     2581 ctcgaccgaa aacgcggcgg cgatttcggc gtcgccgacc agcgcggaaa gaatcggatg
     2641 atcgaaagcg gagatggtca tagctcgtac ctgccgcgcc ggcgccatcc tcccccgcga
     2701 agcgggggag ggggaccatg cgaagcatgg tggagggggc cgcggcctat gaggataaat
     2761 cccaaccgtc ggagccccct ccaccgcgcg ccgacgcgcg cggtccccct cccccgtctt
     2821 tcgacggggg aggatgggct caaatatcga aaaacaccgt ttcgttctcg ccttgcaggc
     2881 ggatgtcgaa atggtacgtc gcccggccgc ccttgtcgct gcgccgggcg atcaagctgt
     2941 cgacgcggct ctgatgttcg agccgggcga ggaccgggca tttggcgttg gcggcggctt
     3001 cgtcggaaaa atacatccgc gtatgcaggc cgacattgat gccgcgggcg acgatccaga
     3061 aggtgacgtg cggcgccatc ggcctcccgt cgacgaaagg gacagggccg ggcttgatcg
     3121 tctcgaagcg gtactcgcca gtgacgtaat cggtcgcctg gcggccaaag ccggcgaaat
     3181 gcggatcggc cttgccgcgc ctttcctgcg gactgttata gagcccggcg gcgtcggcct
     3241 gccagatttc gaccagggcg tccttcagcg gctcgcccaa gccgtcgagg acgcggccga
     3301 cgatcgtgat gcgctcgccc ttggcgtcgc ggccgaccat cgtcctgccg aggtcttgcg
     3361 cgtagacgcc ctcgatgccg gaaaaattcg gcgtcgcgcc gatatggacg tacgggccgg
     3421 cggtctgcga cggcgtctct ttgagatggt cgagcttctg aaccatcaat tgccctcttt
     3481 tttgttctcg aacggcgtcg agcgccggcc gcgcagcacg atgtcgaatt tgtaggcgag
     3541 cgaatccata tgggtcgaat tgttgcggtc gagcgccgcg accagttgtt cgagcgcctc
     3601 gcggtcggga atgccggtgg cgatcgggca ttgccagatc atcggatcgc cctcgaaata
     3661 catctgcgtg atcagccttt gggtaaaagc gtggccgaaa atcgaaaagt ggatatgggc
     3721 cgggcgccag tcgttgaccc ggttcggcca ggggtagggg ccgggtctga cggtgcgaaa
     3781 ccaataacgg ccgtcggaat cggtgatcgt gcggccgcag ccgccgaaat tcggatcgag
     3841 cgg
//
LOCUS       DATGJW010000222         2732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_766808, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000222 DATGJW010000000
VERSION     DATGJW010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2732
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766808"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..390
                     /locus_tag="VKU03_06990"
     CDS             <1..390
                     /locus_tag="VKU03_06990"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HLJ71044.1"
                     /translation="MQEMLYPTSYLKSKGLGKACALITDGRFSGGTSGLSIGHVSPEA
                     AEGGAIGLVEEGDRIEIDIPNRTINLAVSEDELAKRRAAMAAKGKDAWKPAKPRKRAI
                     STALKAYAALTTSASRGAVREIPERLR"
     gene            473..1717
                     /locus_tag="VKU03_06995"
     CDS             473..1717
                     /locus_tag="VKU03_06995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HLJ71045.1"
                     /translation="MKQAALALLPGRSSQVFDVIIAGGGVAGLALACCLKSALGAGVR
                     VLVADPRLGENEGRERAYAVAAGPRRLLERVGAWTAIAPKAQPIVEMAITDGGAHDAA
                     RPTQLRFVERRDEPLAHMAMSDDLTAALYARALALGVECARAEVAGFGLNRYTIEARL
                     AGAAPRARLLVAADGARSKLRDLADIATIGWDYPQSGIVATVAHERDHHGRAEQHFLP
                     AGPFAILPLPGRRSSIVWNESRADADALLALDADDFKRQLEARFTLKLGEIDVVSKPI
                     AIPLRYRLARRFIAERLALIGDAAHIIHPLAGQGLNLALRDVAALAEIVVKQMRLGLD
                     PGAAAPLQEYERARRFDVAASGLGMDAMNRLFSNDVPPLRALRDFGLRLVDRTPALKA
                     LLIEEAAGERGAAPLLLYGETI"
     gene            1728..>2732
                     /locus_tag="VKU03_07000"
     CDS             1728..>2732
                     /locus_tag="VKU03_07000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLJ71046.1"
                     /translation="MTTQRPPWRAATLAAQALGKIDEASRAVVPPLHVTTTFIRDPDN
                     LYRSGNIYGRPDNETVREGEAVIAALEEGAAALLFGSGMSAAVALFLALGPGAHIVAP
                     KVMYWSLRNWLVTEARAFGLAVDLAATEDLSAIAAAIRPGRTKLVWLETPSNPLWSIS
                     DIAGAAELAHRAGARLAVDSTCASPVLTRPLTLGADVAMHSATKYLNGHSDALAGALA
                     FAAEDELMARAKQIRAAHGLILGPFEAFLLIRGMRTVHLRVAAASRNALELAKRLSGH
                     AGVAEVLYPGLPSHPGHDLARRQMSGGFGGMLSIRVAGGEAAAIAAAARLTLWKRATS
                     L"
BASE COUNT          399 a          957 c          937 g          439 t
ORIGIN      
        1 atgcaggaaa tgctctatcc gaccagctat ttgaaatcga agggcctcgg caaggcctgc
       61 gcgctgatca ccgacggccg cttctcgggc ggcacgtccg gcctgtcgat cggccatgtc
      121 tcgccggaag ccgccgaagg cggcgcgatc ggcctcgtcg aggaaggcga ccggatcgag
      181 atcgacattc cgaaccgcac gatcaatctc gccgtctcgg aggacgagct cgccaagcgc
      241 cgcgccgcga tggccgccaa gggcaaggac gcctggaagc cggccaagcc gcgcaagcgc
      301 gcgatctcga ccgcgctgaa ggcgtacgcc gcgctgacga caagcgcgtc gcgcggcgcg
      361 gtgcgggaga ttcctgagcg gctgagatag ggcggcggga tggcgtcaac ctctattgcg
      421 ctccgaccgc cggatttgaa ataatagacc tgccgccatc cccggcggct gaatgaaaca
      481 ggcggcgtta gcgcttttgc ccggtcgctc cagccaggtc ttcgacgtca taatcgccgg
      541 cggcggcgtc gccgggctgg cgctggcctg ctgcttgaag agcgcgctcg gcgcgggcgt
      601 gagggtcctc gtcgccgatc ccaggctcgg cgagaacgag ggccgcgagc gcgcctacgc
      661 cgtcgccgcg gggccgcgcc gcctgttgga gcgggtcggc gcgtggaccg cgattgcgcc
      721 gaaggcgcag cccattgtcg agatggcgat cacggatggc ggcgcgcatg acgcggcgcg
      781 gccgacgcaa ttgcgcttcg tcgagcggcg cgacgagccg ctggcccata tggcgatgag
      841 cgacgatctg accgccgcgc tctatgcgcg ggcgctggcg ctcggcgtcg aatgcgccag
      901 ggccgaggtc gccggcttcg gcctcaatcg ctatacgatc gaggcgaggc tcgccggcgc
      961 cgcgccgcgc gcccggctcc tcgtcgccgc tgacggcgcc cgctccaagc tgcgcgatct
     1021 cgccgacatc gccacgatcg gctgggatta cccgcaatcc ggcatcgtcg cgaccgtcgc
     1081 gcatgagcgc gatcatcatg gccgcgccga gcagcatttt ctccccgccg gccctttcgc
     1141 catcctaccg ctgccgggcc gccgctccag cattgtgtgg aacgagagcc gggccgacgc
     1201 cgacgcgctg ctcgcgctcg acgcggacga tttcaagcgc cagctcgaag cgcgcttcac
     1261 gctcaagctc ggcgagatcg acgtcgtctc caagccgatc gccattccgc tgcgctaccg
     1321 cctggcgcgg cgctttatcg ccgagcgttt agcgctgatc ggcgacgccg ctcatatcat
     1381 ccatcccttg gcgggccagg ggctcaatct ggcgttgcgc gatgtcgcgg ctttggccga
     1441 gatcgtcgtc aagcagatgc ggctcggcct cgatccgggc gccgcggcgc cgttgcaaga
     1501 gtacgagcgc gcgcgccgtt tcgacgtcgc ggcgagcggg ctcggcatgg atgcgatgaa
     1561 ccgcctcttc tccaacgacg ttccgccctt gcgggcgctg cgcgatttcg gcctgcgcct
     1621 cgtcgaccgg acgccggcgc tcaaggctct gctgatcgag gaggcggccg gagaacgcgg
     1681 cgccgcgccg ctgcttctct acggcgagac gatctgatcg gaggctcatg acgacgcaaa
     1741 gacctccctg gcgcgccgcg accctcgcgg cgcaggcttt gggcaagatc gacgaggcga
     1801 gccgcgcggt cgtgccgccg ctgcatgtga cgacgacgtt catccgcgat cccgacaatc
     1861 tctatcgtag cggcaatatc tatggccgcc ctgacaacga gacggtgcgc gagggcgaag
     1921 cggtgatcgc ggcgctcgaa gagggcgccg ccgcgctgct tttcggctcg ggcatgtccg
     1981 ccgccgtcgc cctgttcctg gcgctcgggc cgggcgcgca tatcgtcgcg ccgaaagtca
     2041 tgtactggtc gctgcgcaat tggctggtca ccgaggcgcg ggctttcggc cttgccgtcg
     2101 atctcgcagc gacggaggat ttaagcgcaa tcgccgccgc gatccggccc ggccgcacca
     2161 agctcgtctg gctggagacg ccgtccaatc cgttgtggtc gatcagcgac attgccggcg
     2221 cggccgaact cgcccatcgc gccggcgcca ggctcgcggt cgattcgact tgcgccagcc
     2281 cggtcttgac gcggcctttg acgctcggcg cggatgtggc gatgcattcg gcaacgaaat
     2341 atctcaacgg ccattccgat gcgctcgccg gcgcgctcgc tttcgccgcc gaagacgagc
     2401 tcatggcgcg ggcgaagcag attcgcgccg cgcatggcct gatcctcggg ccgttcgagg
     2461 ctttcctgct catccgcggc atgcgcacgg tgcatctgcg cgtcgccgcc gcctcgcgaa
     2521 acgcgcttga actggccaag cggctatcgg gccacgccgg cgtcgcggaa gtcctctatc
     2581 ccggcctgcc gagccatccc ggccatgact tggcgcgccg ccagatgagc ggcggtttcg
     2641 gcggcatgct gtcgatccgg gtcgcgggcg gcgaagccgc cgcgattgca gcggccgcgc
     2701 gcctgacttt atggaagcga gcgacctcgc tc
//
LOCUS       DATGJW010000223        13738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1389679, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000223 DATGJW010000000
VERSION     DATGJW010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 13738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13738
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1389679"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..170
                     /locus_tag="VKU03_07005"
     CDS             <1..170
                     /locus_tag="VKU03_07005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014330566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLJ71047.1"
                     /translation="TTHTITLGVQQFLGQYVTDWNAVLAALSLAILPVVIIYVIFSRQ
                     LISGITAGAVK"
     gene            183..1271
                     /gene="ugpC"
                     /locus_tag="VKU03_07010"
     CDS             183..1271
                     /gene="ugpC"
                     /locus_tag="VKU03_07010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HLJ71048.1"
                     /translation="MATLRIDKLNKSYGDVQVLQDIDLAINDGEFVVLVGPSGCGKST
                     LLRMIAGLDGASSGDIEIGERRVNNLEPSQRKIAMVFQSYALYPHMNVRKNMTFGLKF
                     SGMKQAEMDRRVADAARILRLEPLLERRPRELSGGQRQRVAIGRAIVREPDVFLFDEP
                     LSNLDAALRVSTRVEIAKLHRLLGATMVYVTHDQIEAMTLADRIVVMNHGKIAQVGRP
                     LDLYYAPANLFVAGFIGSPAMNFFDAHVEKKGAASVTLAGLVVKPFELAVTSFAAPVG
                     AKVKIGVRPEHLGAPASGGFTIDGAVELVERLGETSYAYIRGAGESQIVAEVRGREAP
                     SAGQTVVLGAPERELHLFDENGERIATA"
     gene            1377..2117
                     /gene="msrA"
                     /locus_tag="VKU03_07015"
     CDS             1377..2117
                     /gene="msrA"
                     /locus_tag="VKU03_07015"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018430668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HLJ71049.1"
                     /translation="MFAPKNRFRLSRPSPSSRALLAAALVALTGGLALMAARSSAAEQ
                     ARQIPAPSVDEQPATAQSETAVFAGGCFWGVQGVFQHVKGVVSAVSGYAGGAASTAQY
                     DTVSTGETGHAESVRVTFDPHKVSYGKLLQIYFSVVHDPTELNRQGPDEGAQYRSEVF
                     AETPDQARVAKAYIAELEKAHSFDAPIVTKVEPFKGFYPAEAYHQDYLTLHPNQPYIA
                     INDLPKVAALKRLFPEQYRPDPVLVGAR"
     gene            complement(2170..2880)
                     /locus_tag="VKU03_07020"
     CDS             complement(2170..2880)
                     /locus_tag="VKU03_07020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLJ71050.1"
                     /translation="MRDFGDGVHGGSRRRFSSIKENEASRSAKQSFRAANGKSGLNAA
                     ADRDCRTRGCPAAYVRLSNCFFRTARGTRACSDLLTQKSAIFKRMQSGDPFRQEMRQA
                     MMKTYKGSCHCGAIRYEADIDLAKGAGKCNCTYCLKARAWAVAIKPSEFRLAPGSAPG
                     IAYHKHPQAPLKYHCGVCGVHTHATGDADYMGGAFVQVFVATLDDATPDELLSGPVRY
                     SDGRHNDWMQPPSDIRHL"
     gene            2854..3369
                     /locus_tag="VKU03_07025"
     CDS             2854..3369
                     /locus_tag="VKU03_07025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008124147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1003 domain-containing protein"
                     /protein_id="HLJ71051.1"
                     /translation="MNAVAEIAHRLHDQEMRRLHQLRDARRRRRAETKAKPELTFGER
                     LADAVAARMGSWSFIVIQSLILAIWIVLNVTAYIRQWDPYPFILLNLALSFQAAYAAP
                     IIMMSQNRQAAIDRAAAEADYDVNAKAELEIELLHQKLDLLREQEVSELIQIIRRLEA
                     RLPPTQAASPA"
     gene            complement(3562..4182)
                     /gene="pcp"
                     /locus_tag="VKU03_07030"
     CDS             complement(3562..4182)
                     /gene="pcp"
                     /locus_tag="VKU03_07030"
                     /EC_number="3.4.19.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR00504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0030163 - protein catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyroglutamyl-peptidase I"
                     /protein_id="HLJ71052.1"
                     /translation="MTPTILVTGFAPFDGESVNPSWLAARRLDGWTVAGRVVAAREIP
                     CAFGASIAALEALMDEIKPEAAIAVGQAGGRADLSVERVAVNLDDAERPDNEGWRPID
                     RPVIEGGPAAYFSSLPVKAIVAALREAGLPASVSNSAGGFVCNHLFYGACHLRETRFP
                     AMKAGFIHVPYALEQGARHPGAPTMAIETIVEGLKIAVATTAGAGV"
     gene            4346..5272
                     /locus_tag="VKU03_07035"
     CDS             4346..5272
                     /locus_tag="VKU03_07035"
                     /EC_number="4.1.3.3"
                     /EC_number="4.2.1.41"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009464216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009436 - glyoxylate catabolic process
                     [Evidence IEA];
                     GO_process: GO:0071704 - organic substance metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrodipicolinate synthase family protein"
                     /protein_id="HLJ71053.1"
                     /translation="MPLLDESARGVFLIAATPFKDDGALDLESADRMTDFYLERGADG
                     LTLLGIMGEAPKLTAEESVAFVRRIVRRVDGRVPIIVGVSSPGLASLKALAASAMNDG
                     AAGVLVAAPHTARTDDQIFAYFEGVAETLGPIPFALQDYPLASGVQIAPKVILRIAEA
                     LPTCVMLKHEDWPGLNKITALRAASEKGARRLSILTGNGGLFLPEELARGADGAMTGF
                     AYPEMMVGVVKAHRAGDRDKAQDIFDAYLPLARYEQQQGIGLAVRKYVLAKRGAIASA
                     ALRKPRPALSPADVAEIERLIARQERRLKTLA"
     gene            complement(5256..6077)
                     /locus_tag="VKU03_07040"
     CDS             complement(5256..6077)
                     /locus_tag="VKU03_07040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ71054.1"
                     /translation="MQSQALNKLAPAKPASVGDLVRHWRQLRRKSQLDLACDADISTR
                     HLSFIETGRSTPSRDMVLHLAESLDVPIRERNALLLAAGYAPAYQARDLSDPVLRPVR
                     EAIDLLLSAHEPFPALAVDRHWTLIAANRAVAPLTAGAVADLLKPPVNVMRLSLHPQG
                     VAPRIVNLAEWRDHLLYRLRRQHADTADATLGALIEELAAYPCGPSRPRSADALASAL
                     AVPLRLKTESGILSFLSATMMFDASLDATLSEIALETFLPADAETAAALRLTPAS"
     gene            6199..6561
                     /locus_tag="VKU03_07045"
     CDS             6199..6561
                     /locus_tag="VKU03_07045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007604660.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLJ71055.1"
                     /translation="MTDAAIIAEKYIALWNEADRKRRKELIAKAWTEDATYIDPMMQG
                     SGHDEIDALVGAVHQRFPGHVFALAGKPDGHGDRVRFSWTLGAPDAAPIAKGTDFGIV
                     AGDGRLKAITGFLDYVQA"
     gene            6767..8137
                     /locus_tag="VKU03_07050"
     CDS             6767..8137
                     /locus_tag="VKU03_07050"
                     /EC_number="6.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389422.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004357 - glutamate-cysteine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--cysteine ligase"
                     /protein_id="HLJ71056.1"
                     /translation="MARDVIDASPIESREALVGWLEQGCKPADSFRVGTEHEKIPFYR
                     ADLSPVPYEGERGVKALLEGMQARLGWTPILDEGRLIGLYDESGGGAISLEPGGQFEL
                     SGAPLATIHETAAELDRHLADVAKVGGALGVGFLALGLSPKWSLRETPVMPKGRYQIM
                     ARYMPKVGSRGLDMMFRTATVQANLDFSSEADMVKKMRVGLALQPLATALFANSPFLE
                     GKPNGFLSGRSEIWRDTDNRRAGMLPFAFEPGMGFERYVDYALDVPMYFVKRGAHYHD
                     VAGASFRDLLAGRLAALPGERATMSDWVNHLSTIFPEVRLKRYLEMRGADVGSRAAIC
                     ALPALFVGLFYDSLALDAACDLIKPWRAEDRQRLRDDVPRLALAAKIAGRKLDEIARE
                     ALAIARAGLSRRSRRDAAGRDETIYLAPLEESVETGRVPAAHWLARYAGPWRETIDPV
                     FKEAAY"
     gene            complement(8131..8493)
                     /locus_tag="VKU03_07055"
     CDS             complement(8131..8493)
                     /locus_tag="VKU03_07055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71057.1"
                     /translation="MGRWGLGLLAAVAVLEAASAQEAPPSAERDKIAAAANAHCAALG
                     PGFFAVAGSSACFRISGHISAGVGFAAAGHAPAPPGGAFAEEGVSGDIRFDTPIGPGR
                     IYVHVRDAEGPRWLAGPQ"
     gene            complement(8648..9394)
                     /locus_tag="VKU03_07060"
     CDS             complement(8648..9394)
                     /locus_tag="VKU03_07060"
                     /EC_number="2.1.1.193"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (uracil(1498)-N(3))-methyltransferase"
                     /protein_id="HLJ71058.1"
                     /translation="MARTDFTSPRLFLDAALAEGARLPLGREHANYLLNVLRLGDGAR
                     VLVFNGRDGEFAASLESASRKSAALVVGARIRPQESAPDLDYLFAPLKHARLDYMAQK
                     AVEMGARRLRPVMTRRTQASRVNLDRLRANAIEACEQCGVIWIPEIIGETPLEKALAQ
                     WPAERLLIFCDEEAPLSDPLEALSEAGERGRPLALLIGPEGGFDEEERAAILRLPHVL
                     RLSLGPRILRADTAAVAALALIQARLGDWR"
     gene            10248..11786
                     /locus_tag="VKU03_07065"
     CDS             10248..11786
                     /locus_tag="VKU03_07065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403870.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HLJ71059.1"
                     /translation="MVARVSTVAFEGIEARPVDVQVQIAPGAIAFMIVGLADKAVGES
                     RERVRAALIASGLALPAKRITVNLAPADMPKEGSHYDLPIALAVMAAIGAIPSDALQG
                     YTVIGELALDGAVSAVAGALPAAIAANARGHGLICPAGCGPEAAWASSELDILAPRSL
                     IQLANHFKGTQVLARPAPALRAPSGPSPDLRDIKGQENAKRALEVAAAGGHNLLMSGS
                     PGAGKSMLAQRLPSILPPLSARELLEVSMIHSIAGNLAGGALTDRRPFRAPHHSASMA
                     ALVGGGVHARPGEISLAHQGVLFLDELPEFSPQALDSLRQPIETGEVAIARANHRIVY
                     PARFQLIAAMNPCRCGHASEPGYACSRAPNARCMAQYQARLSGPLLDRFDLTIDVPAV
                     TAADLLLPAPAEGSAEVAGRVAAAREIQAERYRALDLPQVACNAAAPAGIVEQVLDAD
                     ASGLALLREAAERMRLSARGYHRVLKLARTLADLDGGGPVGRAHLAEALSYRAGGERA
                     AIAA"
     gene            11916..12932
                     /locus_tag="VKU03_07070"
     CDS             11916..12932
                     /locus_tag="VKU03_07070"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HLJ71060.1"
                     /translation="MSLAESGRNQRRQQILVPPVRNGRKIGPVLPISDNTSAGLVALL
                     AANQWYRSRRWPAPFDSTEHRLRVGDARDLSWIPDDSIHLVVTSPPYWSLKKYETRDG
                     QLGEILDYEEFLQALDIVWRQCARILVEGGRICCVVGDVCIPRRRTGRHFVMSLHSDI
                     QVRARSLGLDCLTPILWHKIANGVTEAQGNGAGFYGKPYQPGAVVKNDVEYILFMRKP
                     GNYRTTGMLQKALSMLTKEEMQSWFRSFWTDVKGASTRDGHPAPYPAELAERLIRMFS
                     FAGDTVLDPFVGTGSTSVAAIRSGRNSIGVELEPQYLEMAHSRVSAEASRPRLFGAHR
                     AAVI"
     gene            12932..13570
                     /locus_tag="VKU03_07075"
     CDS             12932..13570
                     /locus_tag="VKU03_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71061.1"
                     /translation="MPVTLSQAVDDLLERRTQDPGRLDLLAAFCIEEFKRHGLEGVRG
                     GKADEVGIRGFGRQKDWDLAYVLAGKPRLLVSLKSILKNLAGTVPNRLDDLMGEAANI
                     QQLSPEVVIGYVVIMDEAEDSLRRDGKGTWSDHFESNLRHIAIRRAPLWNQGLIEAAW
                     LIRIDSRKNSGQRISDALDMDAKSTNFFQALLDELYLREPTLKQTRVPPEAL"
BASE COUNT         2398 a         4476 c         4420 g         2444 t
ORIGIN      
        1 acacgaccca tacgatcacg ctcggcgtgc agcagttcct cggccaatac gtcaccgact
       61 ggaacgcagt tttggcggcc ttgtcgctcg ccatcctgcc ggtcgtcatc atctatgtca
      121 ttttctcgcg ccaattgatc agcggcatca ccgccggcgc ggtcaagtag cggaccttcg
      181 tcatggcgac tttgcgcatc gacaagctca acaaatccta tggcgacgtc caggtcctgc
      241 aggacatcga tctcgccatc aatgacggcg agttcgtcgt gctggtcggc ccgtcgggct
      301 gcggcaagtc gaccttgctg cgcatgatcg cggggctcga cggcgcgtcc agcggcgata
      361 tcgagatcgg cgagcggcgc gtcaacaatc tcgagccgtc gcagcgcaag atcgccatgg
      421 tattccaatc ctatgcgctt tatccgcaca tgaacgtgcg caagaacatg acgttcggtc
      481 tgaaattctc cggcatgaag caggccgaga tggatcgccg cgtcgccgac gcggcgcgca
      541 tcctgcgcct tgagcccttg ctcgagcgtc ggccgcgcga gctttccggc ggccagcgtc
      601 agcgcgtcgc gatcggaaga gccatcgtgc gcgagcccga cgtgttcctg ttcgacgagc
      661 cgctctccaa tctcgacgcg gccttgcgcg tctcgacccg ggtcgagatc gccaaattgc
      721 atcgcctgct cggcgcgacc atggtctatg tcacccatga ccagatcgag gcgatgacgc
      781 tggccgaccg catcgtggtg atgaatcacg gcaagatcgc ccaagtcggc cggccgctcg
      841 atctctacta cgcgccggcc aatctcttcg tcgccggctt catcggctcg ccggcgatga
      901 atttcttcga cgcccatgtc gagaagaagg gcgcggcgag cgtcacgctt gccggcctcg
      961 tggtcaagcc gttcgaactc gccgtaacca gcttcgcggc gccggtcggc gcgaaggtga
     1021 agatcggcgt caggcccgag catctcggcg cgcccgcttc aggcggcttc acaatcgacg
     1081 gcgcggtgga gctggtcgag cggctcggcg aaacctccta cgcctatatc cgcggcgcgg
     1141 gcgagagcca gatcgtggcg gaggtgcgcg ggcgcgaagc gccgtcggcg ggacaaaccg
     1201 tcgtgctcgg cgccccagag cgcgagcttc atctcttcga cgagaacggc gagcggattg
     1261 cgacggcgtg agcgcgattg actttccgtt caaatcgccg tgaagctcga cgccgccgta
     1321 acgaaagccc cgaccgtctc gtaaaggcgt cgaagcgacc gctatgggag acgccgatgt
     1381 tcgcgccaaa aaaccgattc aggctcagcc gcccctcgcc ttcgagccgc gcccttctcg
     1441 cagccgctct ggtcgctttg accggcggtc tcgccctcat ggccgcccgc tcctcggccg
     1501 ccgagcaggc gcgccagatt ccggctcctt ccgtcgacga gcagccggct acggcgcaat
     1561 ccgaaaccgc ggtcttcgcc ggcggctgct tttggggcgt gcagggcgtc ttccagcatg
     1621 tgaaaggcgt cgtcagcgcg gtttcaggct atgccggcgg cgccgcttcg accgcgcaat
     1681 acgacacggt gagcaccggc gagaccggcc atgccgagtc ggtgcgcgtc acgttcgatc
     1741 cgcacaaggt cagctacggc aagctcttgc aaatctactt ctcggtcgtc catgatccga
     1801 ctgagctcaa ccgccaaggt ccggacgagg gcgcgcaata ccgctcggaa gtgttcgccg
     1861 agacgccgga ccaggcgcgc gtcgctaaag cctatatcgc cgagctcgag aaagcccatt
     1921 cgttcgacgc gccgatcgtc acgaaagtcg agccgttcaa gggcttctat ccggccgagg
     1981 cgtaccatca ggactacctg accctgcatc cgaaccagcc ctatatcgcg atcaacgatc
     2041 tgcccaaggt cgcggcgctg aagcggctct tccccgagca gtatcgaccc gatcccgtac
     2101 tggtcggcgc gcgctgatcc cggatggcga agctgggcct tctgtgaagg cccagcttat
     2161 cttaaccgcc tagagatgcc gaatatctga cggcggctgc atccaatcgt tgtggcgacc
     2221 gtccgagtac cggacgggtc cgctcaagag ctcgtccggc gtcgcatcgt caagcgtggc
     2281 gacgaacact tgcacgaacg cgccgcccat ataatcggcg tcgcctgtag cgtgagtatg
     2341 aacgccgcag acgccgcaat ggtatttcag cggcgcttgc ggatgcttgt gataagcaat
     2401 tccgggcgcg gagccaggcg caagacgaaa ttcggatggc ttgatcgcca ccgcccaggc
     2461 tcgcgccttc agacaatagg tgcaattgca cttacccgct cccttggcga ggtcgatatc
     2521 cgcttcgtac ctgatcgccc cgcaatgaca acttccttta taggttttca tcatcgcctg
     2581 cctcatttct tgcctgaaag ggtctcccga ttgcatgcgt ttaaaaatgg cgctcttttg
     2641 tgtcaacaaa tcggaacagg cgcgggtccc gcgcgcggtt cgaaaaaaac aattggacaa
     2701 ccgaacataa gcagcaggac aacctcttgt cctacaatca cgatccgccg ccgcgttcag
     2761 gccggacttt ccgttggcgg cgcggaagga ttgcttggca gagcgggagg cttcattttc
     2821 ctttatcgag gaaaaccgcc gccgggagcc gccatgaacg ccgtcgccga aatcgcgcat
     2881 cgtctccacg atcaggagat gaggcggctc catcagcttc gcgatgcgcg ccgccgccga
     2941 cgggcggaga cgaaggcgaa gcccgaactg actttcggcg agcggctcgc cgacgccgtc
     3001 gcggcgagaa tggggtcatg gagcttcatc gtcatccagt cgctcattct ggcgatctgg
     3061 atcgttctca acgtcaccgc ctatatccgg caatgggacc cttatccgtt catcctgctc
     3121 aatctggctt tgtcgttcca agcggcgtac gcggccccga tcatcatgat gagccagaac
     3181 cgccaagcgg cgatcgaccg cgccgcggcg gaggccgatt acgacgtcaa cgccaaggcc
     3241 gaactcgaaa tcgagctcct gcaccaaaag ctcgatctgc tgcgcgagca ggaagtctcg
     3301 gagctgatcc agatcatccg gcggctcgaa gcacggctgc cgccgaccca agccgcaagc
     3361 cccgcctgat cgacgagcga actcgaagaa gcgttaagcg cgagctacgg ccgcccctta
     3421 acgctcggcg cgcgatggct cggtcccgcc gtcattgcaa gcgaagcgaa gcaatccact
     3481 cgtagcgccc gatcaatgga ttgcttcgcc gccgcgctcc tcgcaatgac ggttaatcct
     3541 ccgacttgag cggaaaccgc cttagacgcc ggcgccggcc gtcgtcgcga cggcgatctt
     3601 cagcccctcg acgatcgtct cgatcgccat ggtcggcgcg ccggggtggc gcgcgccttg
     3661 ttcgagcgcg taggggacat ggatgaagcc ggctttcatc gccggaaagc gcgtctcgcg
     3721 caaatggcaa gcgccgtaga agagatgatt gcaaacgaag ccgccggcgg agtttgaaac
     3781 cgaagccggc aggcccgcct cgcgcaaggc cgcgacgatc gccttgaccg gcaggctcga
     3841 aaaataggcg gccgggccgc cctcgatcac cggcctatcg attggccgcc agccttcatt
     3901 gtcgggcctc tcggcgtcgt cgagattgac cgcgacgcgc tcgaccgaga gatccgccct
     3961 gcccccggcc tggccgaccg cgatcgccgc ttccggcttg atctcgtcca tgagcgcttc
     4021 cagcgcggcg atcgaggcgc cgaaggcgca aggaatctcc cgcgcggcga cgacgcggcc
     4081 ggccacggtc cagccgtcga gccgccgcgc cgccagccag gacggattga cgctctcgcc
     4141 gtcgaaaggc gcaaagccgg tgacgaggat ggttggcgtc atgggcgcct tctcgcgttt
     4201 tcgccctatc tgcgcaaggc tcgggccctg cgcaaggcgc cggcgctgcg cagcgcgcag
     4261 agccgacagg cgcagccggc ctatcgcatt tccggcggcg gccttatagg gagcgcgccc
     4321 gccttggaca attcgaggaa atccgatgcc ccttctcgat gaaagcgccc gcggcgtctt
     4381 cctcatcgcc gcgacgccgt tcaaggatga cggcgcgctc gatctcgaga gcgccgaccg
     4441 gatgacggat ttctatctcg agcgcggcgc cgacggcctg accctgctcg gcattatggg
     4501 cgaagcgccc aagctcaccg cggaagaatc cgtcgccttc gttcgccgga tcgtccgccg
     4561 ggtcgacggg cgcgttccga tcatcgtcgg cgtctcctcg cccgggctcg cctcgctgaa
     4621 ggcgctggcg gcgagcgcga tgaacgacgg cgcggcgggc gtgctcgtcg ccgcgccgca
     4681 tacggcgcgc accgacgatc agattttcgc ctatttcgag ggcgtcgccg agacgctggg
     4741 tccgatccca ttcgcgctgc aggactaccc gctcgcctcg ggcgtgcaga tcgcgccgaa
     4801 ggtcatcctg cgcatcgccg aggcgctgcc gacctgcgtc atgctgaagc acgaggactg
     4861 gcccggcctc aacaagatca ccgctttgcg ggcggcgagc gagaaaggcg cgcggcgcct
     4921 ctcgatcctc accggcaatg gcggcctgtt tcttcccgag gagctcgccc gcggcgccga
     4981 tggcgcgatg accggcttcg cctatccgga aatgatggtc ggcgtggtca aggcgcatcg
     5041 cgccggggac agggacaagg cccaggatat tttcgacgcc tatctgccgc tggcgcgata
     5101 cgagcagcag cagggaatcg gcctcgcggt gcgcaaatac gtgctggcca agcgcggcgc
     5161 catcgcgtcg gccgccttgc gcaagcctcg cccggcgctg tcgccggccg atgtcgccga
     5221 gatcgagcgg ctgatcgccc gtcaggagcg gcggctcaag acgctggcgt gaggcgcaac
     5281 gccgccgcgg tttcggcgtc ggccggcaga aacgtctcca gcgcgatttc cgacagcgtc
     5341 gcatcgagcg acgcgtcgaa catcatcgtg gcgctgagga aggacaggat tccgctctcc
     5401 gtcttcagcc tcagcggaac ggcgagcgcg gaggccaggg cgtcggcgct gcgcggccgc
     5461 gacgggccgc agggataggc ggcgagctcc tcgatcagcg cgccgagggt tgcgtcggcc
     5521 gtatcggcgt gctggcgccg cagccggtac aagagatggt cgcgccattc ggcgagattg
     5581 acgatgcgcg gggcgacgcc ttgcgggtgc aggctgagcc gcatcacgtt gaccggcggt
     5641 ttgagcaggt cggcgacggc gccggcggtg agcggagcga ccgcccgatt ggcggcgatc
     5701 aaagtccaat gccgatcgac ggcgagcgcc ggaaacggct catgggccga aagcagaagg
     5761 tcgatcgcct cgcggaccgg gcgcaggacc ggatcgctca agtcgcgcgc ctggtaggcc
     5821 ggcgcgtagc cggcggcgag cagcagcgcg ttgcgctcgc ggatcggcac gtccaggctc
     5881 tcggcgagat ggagcaccat gtcgcggctc ggcgtcgacc ggccggtttc gatgaagctc
     5941 agatgccggg tcgaaatgtc ggcgtcgcag gcaagatcga gctggctctt gcgccgcaat
     6001 tgccgccaat ggcgcacgag atcgccgacg gaggccggct ttgccggcgc aagcttgtta
     6061 agggcctggg attgcatggc cctcaacata gaggcggcgg cgcgccgccg ccattacctc
     6121 cgccgtaatc gaccggcggc gccgtttggg acgatcttcg ccagcgagcc ggaatggtcc
     6181 ggccaaggag actttccgat gaccgacgct gcaatcatcg ccgagaagta tatcgccctg
     6241 tggaacgaag ccgaccgaaa gcggcgcaag gagctcatcg ccaaagcctg gacggaggac
     6301 gcgacctata tcgatccgat gatgcagggg agcggccacg acgagatcga cgcgctggtc
     6361 ggcgccgttc accagcgctt tccgggccat gtcttcgccc ttgccggcaa gccggacggc
     6421 catggcgacc gggtgcgctt ctcctggacg ctcggcgcgc ccgacgccgc gccgatcgcc
     6481 aagggcacgg atttcggcat cgtcgccggc gacggccggc tgaaggcgat caccggcttc
     6541 ctcgattatg tccaagccta aagagcggat cggggcggcc ggtttcggcc gccccgtcta
     6601 tgacgttccg ctcgcatgtc cgaacacggc ttgagggtga gatggccaga gcttacggcg
     6661 gccgtccgcg atcctatccg ccccttcatt gccttgtcgc gcccgtccca ctatttaagc
     6721 gggccgcgag ccggcgtcct ccgcgctcgt ctttccggaa tggtgcatgg cccgcgacgt
     6781 tattgacgcc agtcctatcg agtcgcggga agccctcgtc ggctggctgg agcagggatg
     6841 caagccggcc gattcctttc gggtcggcac cgaacatgaa aagatcccgt tctaccgcgc
     6901 cgatctttcg ccggttccct atgagggcga gcggggcgtc aaagccctgc tcgaaggcat
     6961 gcaagcaagg ctcggctgga cgccgatcct cgacgagggt cgcctgatcg ggctttacga
     7021 cgaaagcggc ggcggcgcga tttcgctcga gcccgggggc cagttcgaat tgtccggcgc
     7081 gccgctcgcc acgatccacg agaccgccgc cgaactcgat cgccacctcg ccgatgtcgc
     7141 gaaggtcggc ggcgcgctgg gcgtcggctt tctcgcgctc ggcctcagcc cgaaatggag
     7201 cttgcgcgag acgccagtca tgcccaaagg gcgctaccag atcatggcgc gctacatgcc
     7261 gaaggtcggc tcgcgcggcc tcgacatgat gttccgcacc gccaccgtgc aggccaatct
     7321 cgatttttcg agcgaggcgg acatggtcaa gaagatgcgg gtcgggctcg cgctgcagcc
     7381 cctcgcgacc gctctcttcg ccaattcgcc tttcctcgaa ggcaagccga acggctttct
     7441 ttccggccgg tcggagattt ggcgcgacac cgacaatcgc cgcgccggga tgctgccttt
     7501 cgccttcgag ccgggcatgg ggttcgagcg ctatgtcgat tatgcgctcg acgtgccgat
     7561 gtatttcgtc aagcgcggcg cgcattatca cgatgtcgcc ggcgcgagct tccgcgatct
     7621 ccttgccggc cggctggccg cgctgcccgg cgagcgcgcg accatgtccg attgggtcaa
     7681 tcatctgtcg acgatttttc cggaagtgcg gctgaagcgc tatctcgaaa tgcgcggcgc
     7741 cgatgtcggc tcgcgagcgg cgatctgcgc cttgccggct ttgttcgtcg gcctgttcta
     7801 cgattccctc gccctggacg cggcctgcga tttgatcaag ccctggcgcg ccgaggatcg
     7861 ccaacggttg cgcgacgacg ttccgcgcct cgccctcgcc gccaaaatcg cgggacgaaa
     7921 gctggacgag attgcgcgcg aggctctcgc catcgcgcgc gccggattat cgcgccggtc
     7981 gaggcgcgac gcggccggcc gggacgagac aatctatctc gcgccgctcg aggaaagcgt
     8041 cgagaccggg cgcgttccag ccgcccattg gctcgcccgc tatgccggcc cgtggcgcga
     8101 aacgatcgat cccgttttca aggaggcggc ctattgaggg ccggccagcc agcgcggacc
     8161 ctcggcgtcg cgcacatgga catagatccg cccggggccg atcggcgtat cgaaacgaat
     8221 gtcgcctgag acgccctcct cggcgaaggc gccgccgggc ggcgctggcg catggccggc
     8281 tgcggcgaag ccgacgccgg cgctgatatg gccgctgatc ctgaagcaag cggacgagcc
     8341 ggcgaccgcg aaaaagccgg ggcccaaagc cgcgcaatgc gcattggccg cggcggcgat
     8401 cttatcgcgc tcggcgctcg gcggcgcctc ctgtgcggag gcggcctcta aaaccgcgac
     8461 cgccgccagc aaccccagac cccaacgacc catcctcgcc cgcctttatc cgtcgcaatg
     8521 tgattcgccg cgttcacccg cgccgttttg atacgccacc ggtgagccga gttcatggcc
     8581 gcttcgcggc ggcgccggcg ctttgcgtcg gatttttgac gtggcgtggc gatttcgcca
     8641 caggcgctca tcgccagtcg ccgagccgcg cctggatgag cgcgagcgcc gcgacggcgg
     8701 cggtgtcggc gcgcagaatg cgcgggccga gcgacaggcg cagaacatgc ggcaggcgga
     8761 gaatcgccgc gcgctcctcc tcgtcgaaac cgccttcagg gccgatcagc aaggcgagcg
     8821 gccggccgcg ctcgcctgcc tcggacaagg cttccagcgg atcgctcagc ggcgcctctt
     8881 cgtcgcaaaa aatcagcagg cgctccgccg gccattgggc gagcgccttc tcgagcggcg
     8941 tttcgccgat gatctccggt atccagatca cgccgcattg ctcgcaggct tcgatcgcat
     9001 tggcgcgaag gcgatcgagg ttgacgcggc ttgcttgcgt gcggcgggtc atgaccggcc
     9061 gcagccgccg cgcgcccatc tccaccgcct tctgcgccat gtagtcgagc cgggcgtgct
     9121 tcagcggcgc gaacagatag tcgaggtcgg gggcgctctc ttgcggcctg atccgcgcgc
     9181 cgacgacgag cgccgcgctc ttccgcgagg cggattcgag ggacgcggcg aattcgccgt
     9241 cgcggccgtt gaagacgagg acgcgggcgc cgtcgccgag gcgcagcacg ttgagaagat
     9301 agttcgcgtg ctcgcggccg agcggcaggc gcgcgccttc ggccaaggcc gcgtcgagga
     9361 agaggcgtgg gctggtgaaa tcggtgcgcg ccatgaagct tgcgagagtt cggatcgagc
     9421 ggcgtcgtac tgacgatagc gcccggagcg tgaggatgct cccgcccgtc ctgcttggtc
     9481 ttcctgtccg tgacggtccc gtcgcccgcc ccgcccaaca ttcggcgccg ccggagatgg
     9541 cgaccgcaga tttggaaaac ctttggagcg cgcgaaaaag cgcgtctggc aaaatttcgg
     9601 ggttcgaaaa acccctttct cattaaggag gccgcaatgc gcgcagcaag acttttccaa
     9661 atttttccaa actttttctt ggccgttttg agcgatttca ggaggttagc tggaaaaaaa
     9721 aaatgaaaac gcgtttttcg tcgtcctggc gatcgcgcca aaagcattat aggcgaagcg
     9781 atgcggttga aggagaaatt tttcgtacca ttgtcgtaag ctcctgaata gaaaggataa
     9841 atctacgaat cccagggctt gatcctccaa ccctggttcg acgccggcga cgggcaagcc
     9901 gaaggcgttt tgcagtcgcg gcgaagcctg aaatcaacgt cccttttcga attgtcctgc
     9961 ggctcggcga tatgtcgacg tacgttctat gcggcggcgt cacgccgcct aatcgccgtc
    10021 agccgctatc gccccgccaa agtacgaaca agccgatatc cgctgggccg cgccatggcg
    10081 ggccttcgct tccgctccgg cccgtacggc tgactgcgac cggccgagag gggcggccaa
    10141 ggaccggaaa acttttgcgc caatcccctt gctttctctg cgatgttctg caaatgttct
    10201 aacaaccgag cgttgtggcg acggctgggc ggagaaaggg gggcggcatg gtcgcgcgcg
    10261 tatcgacagt ggccttcgag ggcatcgagg cgcgccccgt cgacgtgcag gtgcagatcg
    10321 cgccgggcgc catcgctttc atgatcgtcg gcctcgccga caaggcggtg ggggaatcgc
    10381 gcgagcgggt ccgcgccgcg ctgatcgcct ccggtctcgc tttgccggcc aagcgcatca
    10441 ccgtcaatct cgccccggcc gacatgccga aggaaggcag ccattacgac ctgccgatcg
    10501 cgctcgcggt gatggccgcg atcggcgcga tcccgtcgga cgcgttgcag ggctacacgg
    10561 tcatcggcga actggcgctg gacggcgccg tgagcgccgt cgccggcgcg ctgccggccg
    10621 ccatcgccgc caatgcccgc ggccacggcc tcatctgccc ggccggctgt gggccggaag
    10681 cggcctgggc gtcgagcgag ctcgacatcc tggcgccgcg ctcgctcatt cagctcgcca
    10741 accacttcaa gggaacgcaa gtcttggcgc ggccggcccc ggctttgcgc gcgccgagcg
    10801 gcccctcgcc ggaccttcgc gacatcaagg gccaggaaaa cgccaagcgg gcgctcgaag
    10861 tggcggcggc gggcggccac aaccttctga tgagcggctc gccgggcgcg ggcaagtcga
    10921 tgctcgcgca gcgattgccg tcgatcctgc cgccgctctc ggcgcgcgaa ttgctcgaag
    10981 tctcgatgat ccattcgatc gccggcaatc tcgccggcgg cgcgctgacg gaccggcggc
    11041 cgtttcgcgc gccgcatcat tccgcctcga tggcggcttt ggtcggcggc ggcgtccatg
    11101 cgcggcccgg cgagatttcg ctcgcccatc aaggcgtgct gtttctcgac gaattgccgg
    11161 aattcagccc gcaggcgctc gacagcctgc gccagccgat cgaaacgggg gaagtggcga
    11221 tcgcccgggc caatcatcgc atcgtctatc cggcgcgctt tcaactgatc gcggcgatga
    11281 atccctgccg ttgcggccat gccagcgagc ccggctatgc ctgctcgcgc gcgccgaatg
    11341 cgcgctgcat ggcgcaatac caggcgcggc tttcgggccc tttgctcgac cggttcgacc
    11401 tgaccatcga cgtgccggcg gtcacggccg ccgaccttct gctcccggcg ccggcggagg
    11461 gctcggccga ggtcgccgga cgcgtcgccg cggcgcgcga gatccaagcc gagcgctatc
    11521 gcgcgctgga cttgccgcag gtcgcctgca acgcggcggc cccggcgggc attgtcgagc
    11581 aggttctcga tgcggacgct tcggggctgg cgctgctgcg cgaggcggcc gagcggatgc
    11641 ggctttcggc gcgcggctat caccgcgtgc tcaagctcgc ccgcaccctc gccgatctcg
    11701 atggcggcgg ccccgtcggc cgcgcccatc tcgccgaggc tttatcgtac cgcgccggcg
    11761 gcgagcgcgc ggcgatcgcg gcgtgagggg gcgtttttgt ctcgatgcaa tctcatttgt
    11821 ggacaaatat ccgcatgcgg acatcccatg atctgggcgc attggaaact acgcccctgt
    11881 aagctattag aagtttcata ttaaggagag gacaaatgtc attggcggaa tcaggacgca
    11941 accagcgccg ccagcaaatc cttgttcctc ctgtgcgcaa tgggaggaaa atcggtcccg
    12001 tcctgccgat ttcggacaat acatcagcag gtctcgtggc gctgttagcg gcaaatcaat
    12061 ggtaccgtag tcggcgatgg cccgctccct tcgactccac ggaacaccga cttcgtgtcg
    12121 gagacgcccg tgacttgagt tggattcccg acgactcgat tcatctcgtc gtaacatcgc
    12181 cgccgtattg gagcttaaaa aagtacgaga caagagacgg gcagctcggt gaaatccttg
    12241 attacgaaga atttctccaa gcgctcgaca ttgtttggcg tcagtgcgcg cgaattttgg
    12301 tagaaggcgg caggatctgt tgcgtagtgg gtgatgtttg catcccccgc cgccgtactg
    12361 gaaggcattt cgtaatgtcg cttcacagtg atattcaggt acgggcgcgc agcctcggct
    12421 tggactgcct cactcctatc ctttggcaca aaattgcaaa cggcgtgacc gaggcgcaag
    12481 gcaatggcgc cggtttttat ggaaagcctt accaaccagg cgcagtcgtc aagaacgatg
    12541 tcgagtacat actttttatg cgcaaaccag gtaactatcg cacgacaggg atgcttcaaa
    12601 aagccctttc gatgctgacg aaagaggaga tgcagagttg gtttagatcg ttctggacgg
    12661 atgtaaaggg agcgtcgacc cgcgacggcc acccggcgcc gtatccggca gaactcgcag
    12721 agcgacttat tcgaatgttt tcatttgctg gcgacactgt tcttgaccct tttgtcggaa
    12781 cggggtcaac gagcgtggcg gctattcgaa gcggacgaaa ttcaattggg gttgagcttg
    12841 agccgcaata tctcgaaatg gcgcactcta gagtttctgc ggaagcatca cgtcccagac
    12901 tcttcggggc gcatcgggcg gctgtgattt gatgcccgtc accctatcgc aggccgtaga
    12961 cgatttgcta gagcgacgaa cccaagaccc gggacggctc gatttactcg cggctttttg
    13021 cattgaagag ttcaagcgcc atggcttgga gggagttcgc ggcgggaagg ccgacgaggt
    13081 cggcattcga ggttttggcc ggcagaaaga ctgggattta gcttatgttt tggcagggaa
    13141 gccacgactt ttggtgtcct taaaatccat cttaaaaaac ttggctggca ctgttcccaa
    13201 tcgtctggat gatttgatgg gagaagcggc gaacattcag cagctctcgc cggaagtagt
    13261 gatcggctac gtcgtcatta tggatgaagc tgaggattcg ctacgccgag atgggaaggg
    13321 tacctggagc gaccactttg aatccaatct tcgccacatc gccattaggc gtgcgccact
    13381 atggaatcaa ggcctaatcg aagctgcatg gttaatccgc atcgacagcc gtaagaactc
    13441 gggccagcga atatccgatg ctttagatat ggacgccaag agcaccaact tttttcaagc
    13501 cctattggat gagttgtatt tgcgggagcc gacgctgaag caaacccgcg tgcccccaga
    13561 ggctctctga cagcagcgct cccactgtcg ttccggcgaa aagcggcggc ggcgttcgat
    13621 acgggcgggc cggcttggct gtcgctcgct ttgatccatc gcggaaacgc tttctcaacc
    13681 gcgctctgcc aatctcgacg ttctgccgcc agccgtttcg gcgcgaggcg cggcctca
//
LOCUS       DATGJW010000224         2518 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_766922, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000224 DATGJW010000000
VERSION     DATGJW010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2518)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2518)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2518
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_766922"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VKU03_07080"
     CDS             <1..490
                     /locus_tag="VKU03_07080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018449077.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ71062.1"
                     /translation="PVFKANPGIKILAETNTAYNAAPAQEAVTNLLFSHPKIDGVWSQ
                     GGALSAGAVTAFEKQGRKLVPITGENYRPFLEMWKQKKLTAWATQQPNWLAALAVYAT
                     VKALQGEDIPSYINVPLPIIDDSNLYSYLARAKDFAADGYIYSPYDTKLFDQLIANSR
                     KK"
     gene            550..2076
                     /locus_tag="VKU03_07085"
     CDS             550..2076
                     /locus_tag="VKU03_07085"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011069183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ71063.1"
                     /translation="MRQAAAVLGARNVSKAFFGNPVLRDVSIELAAGRIHALLGENGA
                     GKSTLINLLSGTLAPDSGDILFDGEKIAALTPREAHRRGVAVVQQELSLAPHLSIAEN
                     IGLGAYHRRGALIDYGELAAAAEEIGGKLGLVEPPDTPVEQLPLGRRQIVEIAKALFI
                     KPRVLIFDEPTSSLAAPDVAMLMRLIRALRDEGAAILYISHRLNEILDFCDYVTVLKD
                     GAVTADRPLAGLDPTGLVRLMVGRDPQNLFPPYVSHATGELALQTRNFRTAAARAIDF
                     ELRRGEILGIGGLVGHGQEDFMMGLYGAVPAGADQFRVFANGREARAAPFASVAEANA
                     VGIAYVPADRRKEGLLLPHSIDFNLLLPLFGRSATARRRRAEETAITERLARRLNIRG
                     DRGRPAQALSGGNQQKVAVSKWLPLEPSVLLLNDPTRGVDVETKREIYVALQEMAAEG
                     KAVVLLSTDTPELVHLCDRVMVFHKGRVAATLAHAEASEEAIVSAAMGVRRPLSGEAA
                     "
     gene            2073..>2518
                     /locus_tag="VKU03_07090"
     CDS             2073..>2518
                     /locus_tag="VKU03_07090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71064.1"
                     /translation="MTAKKGTRLYWRIQWRRNGGLGALFLVLVLFAIVYAILFPGIFA
                     VGAASKFVQGWFPTAMVAAAEAIVMLTGGIDLSVGPMVSLGSVVAASTMQGPLGILGG
                     VVSVVALGAGVGAIVGAFVAIGRFPAIIVTLALSFVLRGVALLIMPR"
BASE COUNT          396 a          864 c          824 g          434 t
ORIGIN      
        1 gccggtgttc aaggccaatc ccggcatcaa aatcctcgcc gagaccaaca ccgcctacaa
       61 cgccgcgccg gcgcaggagg ccgtcaccaa tctgctcttc agccatccga agatcgacgg
      121 cgtttggagc cagggcggcg cgctttccgc cggagcggtc acggcgttcg agaagcaagg
      181 ccgcaagctc gtgccgatca ccggcgagaa ctatcggccc ttcctcgaaa tgtggaagca
      241 gaagaagctc accgcttggg cgacgcagca gccgaactgg ctcgccgccc tcgccgtcta
      301 cgccacggtc aaagcgctgc aaggcgagga tattccgtcc tatatcaacg tgcccctgcc
      361 gatcatcgac gacagcaatc tgtacagcta cttggcgcga gccaaggact tcgccgccga
      421 cggctatatc tactcgccct atgatacgaa gctgttcgac cagcttatcg ccaacagccg
      481 caagaagtag catttaaggg aagcgattag ggcggagcgc gggtgacgga gcgcgtcaag
      541 gggcacggcg tgcggcaggc ggcggccgtc ctcggcgcgc ggaacgtctc caaggcgttc
      601 ttcggcaatc ctgtgcttcg cgacgtctcg atcgagctcg ccgccggccg catccacgcc
      661 ttgctcggcg agaacggcgc cggcaagagc acgctcatca acctgctctc gggcacgctg
      721 gcgcccgaca gcggcgacat tctcttcgac ggcgagaaga ttgcggcgct gacgccgcgc
      781 gaagcgcatc gccgcggcgt cgccgtcgtc cagcaggagc tgagtcttgc tccccatctc
      841 tcgatcgccg agaatatcgg cctcggcgcc tatcatcgcc gcggcgcgct gatcgattac
      901 ggggagctcg ccgccgcggc cgaagagatc ggcggcaagc tcggcctggt cgagccgccc
      961 gacacgccgg tcgaacaatt gccgctcggc cggcggcaga tcgtcgagat cgccaaggcg
     1021 ctgttcatca agccgcgcgt tctcatcttt gacgagccga cctcctcgct cgccgccccc
     1081 gacgtcgcga tgctgatgcg gctcatccgc gccttgcgcg atgagggcgc cgcgattctc
     1141 tacatctcgc accggctcaa cgagatcctc gatttctgcg actacgtcac ggttctgaag
     1201 gacggcgcgg tcaccgccga tcgcccgctc gccggcctcg atccgaccgg cctcgtccgc
     1261 ctgatggtcg gccgcgaccc gcagaacctg ttcccgccct atgtttcgca cgcgaccgga
     1321 gagttggcgc tgcagacgcg gaatttccgg acggccgcgg cgcgcgccat cgacttcgaa
     1381 ttgcgccgcg gcgagattct gggaatcggc gggctcgtcg gccatgggca ggaagatttc
     1441 atgatgggcc tttacggcgc cgttcccgcc ggcgccgacc agttccgcgt cttcgccaat
     1501 ggccgcgagg cgcgcgccgc gcctttcgcc agcgtcgccg aagcgaacgc tgtcgggatc
     1561 gcctatgtgc cggccgatcg ccgcaaggag ggtttgctcc tgccgcattc gatcgatttc
     1621 aatctgttgc tgcccctgtt cggccgctcc gccaccgcgc gccgacgccg ggccgaggag
     1681 acggcgatca ccgagcgcct ggcgcggcgg ttgaacatcc gcggcgatcg cggccggccg
     1741 gcgcaggccc tttcgggcgg caaccagcag aaggtcgccg tgtcgaaatg gctgccgctc
     1801 gagccgtccg tcctccttct gaacgatccg acccgcggcg tcgacgtcga aaccaagcgg
     1861 gagatctacg tcgccttgca agaaatggcg gcggagggga aggcggtcgt tctgctgagt
     1921 acggacacgc ctgaactcgt ccacctctgc gaccgggtga tggttttcca caaaggccgc
     1981 gtcgccgcca cgctggccca tgccgaagcg tcggaagagg cgatcgtctc ggccgcgatg
     2041 ggggtgcgca ggcccctcag cggagaagcg gcgtgaccgc gaagaaaggg acgcggctct
     2101 attggcgcat tcagtggcgg cgcaacggcg gcctcggcgc gctgtttctc gtgcttgtcc
     2161 tgttcgcgat cgtctacgcc atcctgttcc cgggcatatt cgccgtcgga gcggcgtcga
     2221 aattcgtgca gggttggttt ccgacggcga tggtcgcggc ggcggaagcg atcgtcatgc
     2281 tcaccggcgg catcgatctt tcggtcggcc cgatggtgag cctcggctcg gtcgtcgcgg
     2341 cctcgacgat gcaagggccg ctggggatat tgggcggcgt tgtcagcgtc gtcgcgctcg
     2401 gcgccggcgt cggcgcgatc gtcggcgcat tcgtggccat cggccggttc ccggcgatca
     2461 tcgtgaccct cgccctctcc ttcgtcctgc gcggcgttgc gctgctgatc atgccgcg
//
LOCUS       DATGJW010000225         2652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_353232, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000225 DATGJW010000000
VERSION     DATGJW010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2652
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_353232"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2000
                     /gene="pbpC"
                     /locus_tag="VKU03_07095"
     CDS             <1..2000
                     /gene="pbpC"
                     /locus_tag="VKU03_07095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009274 - peptidoglycan-based cell wall
                     [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding protein 1C"
                     /protein_id="HLJ71065.1"
                     /translation="ASLGPLDLASSREGSAVVVDRDGRLLRPFTLPDGRWRLPATTHD
                     VDPRYLAMLVAYEDGRFQEHDGVDVRALVRAGVQWLTHGHVVSGGSTLTMQAARLMEP
                     RPGRTLAAKLRQIARALEIEREVGKSGALDRYLTLAPFGGNLEGVRAASLAYFGKEPR
                     RLTLAEAALLVALPQSPEARRPDRFPAAARAARDRVLARVAARGIISEGDAAAAKREP
                     VPEARLAFPALAAHAAEEAVAADGHAMVIRLAIDARLQAKLETLARESVARLGPKLSA
                     AIVVIDNMTGEIRARVGSAGHDDPSRDGAIDMSRFARSPGSALKPFIYALAFEEGLAH
                     PETRLFDRPMRYGAYAPQNFSLGYEGAVTARKALQMSLNLPAVELLADVGPATFLARL
                     HSAGAEIALPKDTPIGLAIGLGGLGISLTDIARLYAGLARGGEAPPLIERLDGSPPAL
                     GPRRVTDPVAAWYVADILRGAPAPANALKGRIAFKTGTSYGYRDALAIGFDRRTTIAV
                     WVGRPDNGPTPGLIGREAAAPILFDAFERLGRSIEPIAPPKGVLRASVTADLPPPLRR
                     LRQDAPRPFAAPEAAQLKIAFPPDGALIDLGLKERGRDNKLALKALGGSPPFAWFVNG
                     EPVGEAEIRRQSAWKPDGAGFARVSVTDSKGASDAVTIRLE"
     gene            complement(2461..>2652)
                     /locus_tag="VKU03_07100"
     CDS             complement(2461..>2652)
                     /locus_tag="VKU03_07100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71066.1"
                     /translation="KEVIDGHGGIWQTPLLLFIASIVFQHVQASKQGAPAAPREGGGP
                     SGNLAEFAKSIRPIKRPQS"
BASE COUNT          397 a          908 c          934 g          413 t
ORIGIN      
        1 cggcgagcct cgggccgctc gatctcgcct cctcgcgtga aggttcggcg gtcgtcgtcg
       61 accgcgacgg ccggctgctc cggcccttca ccctgcccga cgggcgctgg cgcctgccgg
      121 cgaccacgca cgacgtcgat ccgcgctatc tcgcgatgct tgtcgcctat gaggacgggc
      181 ggttccagga acacgacggc gtcgacgtcc gcgcactggt ccgcgccggc gtgcagtggc
      241 taacccatgg ccatgtggtg tctggcggct cgacgctcac aatgcaggcc gcgcggctga
      301 tggagccgcg gcctgggcgc actctcgccg ccaagctacg ccagatcgcg cgcgccctcg
      361 agatcgaacg cgaggtcgga aaatcgggcg cgctcgatcg ctatctgacc cttgctccgt
      421 tcggcggcaa tctcgagggc gtgcgggcgg cctcgctcgc ctatttcggc aaggagccgc
      481 ggaggctcac cctggccgaa gcggcgctgc tggtcgcgct gccgcagtcg ccggaagcgc
      541 gccggccgga tcgttttccc gccgcggcgc gcgctgcgcg cgaccgcgtg cttgcccggg
      601 tcgcggcgcg cggaatcatc agcgaaggcg acgccgcggc ggcgaagcgc gagccggtcc
      661 ctgaagcgcg gctggccttt ccggcgctcg ccgcccacgc cgccgaggaa gcggtcgccg
      721 ccgacggcca tgcgatggtc attaggcttg ccatcgacgc gcgtctgcag gcgaagctgg
      781 aaaccctggc cagggagagc gtcgcgcggc ttgggccgaa gctgtcggcg gcgatcgtgg
      841 tcatcgacaa catgaccggc gaaattcgcg cccgtgtggg ctcggcgggc catgacgatc
      901 cctcgcgcga cggcgcgatc gacatgtcgc gcttcgcccg ctcgccgggc tcggcgctga
      961 aacccttcat ctatgcgctg gccttcgagg agggcctggc ccatccggag acgcggctgt
     1021 tcgaccggcc catgcgctac ggggcctatg cgccgcagaa tttcagcctc ggttacgagg
     1081 gcgcggtgac ggcgcgcaag gccttgcaga tgtcactcaa ccttccggcg gtcgagcttc
     1141 tggccgacgt cggccccgcg accttccttg cgcggttgca cagcgccggg gccgaaatcg
     1201 cgctgccgaa agatacgccg atcggcctcg ccatcggact cgggggcctg gggatatcgc
     1261 tcaccgatat cgcccggctc tacgccggcc tcgcgcgggg cggcgaggcc ccgccgctga
     1321 tcgagcggct cgatggctca ccgccggccc tcgggccgcg ccgggtgacc gatccggtcg
     1381 ccgcctggta tgtcgccgac atcctgcgcg gcgcgccggc gccggcgaac gcgctcaaag
     1441 gccggatcgc cttcaagacc ggcacctcct acggctatcg cgatgcgctg gcgatcggct
     1501 tcgaccggcg gacgacgatc gcggtctggg tcgggcggcc ggacaacggc ccgacgccgg
     1561 ggctgatcgg ccgggaggcc gcggcgccga tcctgttcga cgccttcgag cggctggggc
     1621 ggagcatcga gccgatcgcg cccccgaagg gcgtgctcag agcgagcgtc accgccgatc
     1681 tgccgccgcc gttgcgccgc ctgcgccagg acgctcccag gccgtttgct gctcctgagg
     1741 cggctcagct caagatcgcc ttcccgcccg atggcgcctt gatcgacctc ggcctcaagg
     1801 agcgcgggcg cgacaacaag ctggcgctga aggcgttggg cggctcgccg cccttcgcgt
     1861 ggttcgtcaa tggcgagccg gttggcgagg ccgagatcag gcgccaatcg gcctggaagc
     1921 ccgacggcgc cggcttcgcc cgcgtgtcgg tgaccgactc gaagggcgcg tccgacgcgg
     1981 tgacgataag gctggagtga tgccggattc ggttgctgag atcggccggc gtttcccgcg
     2041 aaagcgcccg ctgaaaggcc gggacgctcg acaagaaaaa tcaacaatgt cttgacaaac
     2101 aggatggggt cacgatagta ttggttttcg gaagtagacc aaaacccggc ttggctcggt
     2161 ccccggcctc atgcttcaag gagcccgcaa aagcgggcgt ctcgaagagc ctgccccgag
     2221 cttgccgagg agatgatcga gcaggcggcg cgggccgtgc ggatctggat cgccctccaa
     2281 gaccgcgcct tcggcgtttc ttgaggcgcg aggtggggaa agccgagtga gtcgaaaatg
     2341 gcgccgcaag ccattgaaaa cgctcaaaac ggactcggga atggttgatc cgccgccggc
     2401 tcgcggaagg gagaatcgat caggggcttg gttccggtgg gatggccttg ccgcgcgccc
     2461 tcaagactga ggacgcttga tcgggcgaat cgatttcgcg aactccgcga gattgccgga
     2521 cggtccgccg ccttcacgcg gcgcggccgg cgctccttgc ttcgaagcct gcacgtgctg
     2581 gaacacgatg ctggcaatga aaagaagcaa cggcgtttgc cagatgcccc cgtggccgtc
     2641 gatcacttcc tt
//
LOCUS       DATGJW010000226         2108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_560363, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000226 DATGJW010000000
VERSION     DATGJW010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2108
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_560363"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(137..763)
                     /gene="cmk"
                     /locus_tag="VKU03_07105"
     CDS             complement(137..763)
                     /gene="cmk"
                     /locus_tag="VKU03_07105"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HLJ71067.1"
                     /translation="MIIAIDGPAASGKGTLARRLAVHYGLPHLDTGLLYRATAAALID
                     NDRDLQDVDAAVAAARGLALTDFDEERLRRREMSEAASIVAAMPPVRQALIAAQRSFA
                     ARPGGAVLDGRDIGTVICPNADVKIFVVASPEARAQRRALELAARGEKFDYATILGEI
                     LKRDARDSGRDAAPLKPASDAVLLDSTRLNVEAVFQAARRIVEEKIRG"
     gene            complement(834..>2108)
                     /gene="aroA"
                     /locus_tag="VKU03_07110"
     CDS             complement(834..>2108)
                     /gene="aroA"
                     /locus_tag="VKU03_07110"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HLJ71068.1"
                     /translation="LRPPGDKSISHRAFIFGLLSVGATEIEGLLEGDDVLRTGQACKT
                     LGARIERLGEGRWRVNGLGIGSILPPEETLDFGNAGTGSRLMMGVVGGQGVTARFDGD
                     ASLRKRPMRRILDPLALMGVEILEQAEGGRCPLVIKGTSEPAPIVYRTPVASAQIKSA
                     VLLAGLNAPGETTVVESEASRDHTEKMLRHFGAEVAVAAEGENGRRITLKGRPELRPA
                     PVVVPADPSSAAFPIVAALITPGSDIVVEGVMMNPLRTGLIATLIEMGGRIEALDRRV
                     EGGEEVADLHVRAGPLTGVDVPAARAPSMIDEYPILAVAAAFAEGETRMRGLHELRVK
                     ESDRLAAVAAGLAAAGVSHAIEGDDLIVRGRGEAPGGGLVATHLDHRIAMSFLVMGLA
                     SRKPMAIDDSRMIATSFPTFQPLMASLGARFS"
BASE COUNT          350 a          760 c          688 g          310 t
ORIGIN      
        1 tcgggccgac gaccatagga aagcgcgctc aaaaccgagc ccgcgcagac ggcttcgcgc
       61 aaccctgaga taggcagccc cggcgccctt gtcaatgcag ccaaatccgc cgcgccccgc
      121 tcgccgtcag gagcgttcaa ccgcggattt tctcttcgac aattctccgc gccgcctgaa
      181 aaaccgcctc cacgttcaac cgcgtcgaat cgagcaaaac ggcgtccgac gccggtttca
      241 atggggcggc gtcgcgccca gaatcgcggg cgtcgcgctt gaggatttcg cccaggatcg
      301 tcgcataatc gaatttctcg ccgcgcgcgg cgagctccag agcgcggcgc tgcgcccgcg
      361 cctccgggct ggcgacgaca aaaattttca cgtccgcgtt ggggcaaatc actgtcccga
      421 tgtcccgccc gtcgagaacc gctccgcccg gccgggccgc gaagctgcgc tgggccgcga
      481 tcaaagcctg tcggaccggc ggcatggcgg cgacgataga ggccgcctcg ctcatctcgc
      541 ggcggcgcag ccgctcctcg tcgaaatccg tcagagcgag gccgcgcgcc gccgccactg
      601 ccgcgtccac gtcctggagg tcgcgatcgt tgtcaatcag ggcggccgca gtcgcccgat
      661 agagcaagcc ggtgtcgaga tgcggcaggc cataatggac cgcgagacgg cgcgccagcg
      721 tccccttgcc ggaggccgcc gggccgtcga tggcgatgat catcctgttt cagtcccagc
      781 ggcgccggag gcgctcttac agcctggcga agagtctgtc acggcccggc gcttcagctg
      841 aagcgcgcgc cgaggctcgc catcagcggc tgaaaggtcg ggaagctcgt tgcgatcatt
      901 cgcgaatcgt cgatcgccat cggctttcgc gaggcgaggc ccatgaccag gaagctcatc
      961 gcgatgcggt gatcgagatg ggtggcgacg aggccgccgc ccggcgcttc gccgcggccg
     1021 cgcacaatga gatcgtcgcc ctcgatcgca tgggagaccc ccgccgcagc gagacccgcg
     1081 gcgaccgccg ccagccggtc ggattccttg accctcagct cgtgcaggcc gcgcatgcgc
     1141 gtctcgccct cggcgaaagc ggcggcgacg gcgaggatcg gatactcgtc gatcatcgag
     1201 ggcgcgcgcg cggccggaac gtcgacgccg gtcaggggac cggcgcgcac gtgaagatcg
     1261 gcgacctctt cgccgccctc gacccggcgg tccagcgcct cgatccgccc gcccatttca
     1321 atcagcgtcg cgatcaggcc ggtccgcagc gggttcatca tcacgccttc gacgacaatg
     1381 tccgagccgg gcgtgatcaa agccgcgacg atcggaaagg cggccgagga cggatcggcc
     1441 ggcacgacga ccggcgccgg ccgcaattcc ggccggccct tcagcgtgat cctgcggccg
     1501 ttctcgccct cggccgcgac ggcgacctcg gcgccgaaat ggcgaagcat tttctcggta
     1561 tgatcgcgcg acgcttcgct ttcgacgacg gtcgtctcgc cgggcgcatt gaggccggca
     1621 agcagcaccg cggatttgat ctgggccgag gcgaccggcg tgcgatagac gatcggcgcc
     1681 ggctcgctgg tccccttaat caccagcggg cagcgcccgc cctcggcttg ctcgagaatt
     1741 tccaccccca tcaacgccag cggatcgaga atgcggcgca tcggccgctt gcgcaaagag
     1801 gcgtcgccgt cgaaccgcgc cgtgacgccc tgcccgccga ccacccccat catcaagcgc
     1861 gagccggtgc cggcattgcc gaaatcgagc gtctcctcgg gcggcaagat cgagccgatt
     1921 ccgagcccgt tgacgcgcca gcgaccctcg ccgagacgtt cgatgcgcgc gcccagggtt
     1981 ttacaggcct gcccggtgcg cagcacgtcg tcgccttcca gcaagccctc gatctccgtc
     2041 gcgccgacgc tcaacaagcc gaagatgaag gcgcggtgcg agatcgattt atcgcccgga
     2101 gggcgcaa
//
LOCUS       DATGJW010000227         9020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_456039, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000227 DATGJW010000000
VERSION     DATGJW010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9020
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456039"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            185..1165
                     /locus_tag="VKU03_07115"
     CDS             185..1165
                     /locus_tag="VKU03_07115"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLJ71069.1"
                     /translation="MARAGKTDAILVIKLGSLGDFVQCFDAFHDIRAHHSGARIALLT
                     TPPFAALARRSPWFDEVWTDGRPDWTDLSAWARLIFRLRRARFARVYDLQYNRRSAFI
                     FRALGGAKGPLWLGKAKGCAIPEPLYPAEAGNAERLRLHLQSAGAPSAGEPDLSWLAD
                     DIAGLAPPDPFVLFVPGCSPHLPQKRWPAENYAALGRRLAARGYGVALAGTKADREAV
                     EAIRARFEGAIDLCGRTNLFQLASLARAAKAVVGNDTGPMFLAAKIGAPTLTLMSAHT
                     DEKRSAPPGPAASWLKRDDLAALGVDEVESALNLPARNPPPSKTGEGDRA"
     gene            complement(1203..2444)
                     /locus_tag="VKU03_07120"
     CDS             complement(1203..2444)
                     /locus_tag="VKU03_07120"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ71070.1"
                     /translation="MDEARPRRVAVIRPYAALPSEGGANDRYVNLCEQLIALDAAPEF
                     FCSDFVHNSKQRRSAAAIALNQRRLPYLRQIRSFAYRRNLSLARIAHEAWFGLKAFVR
                     IAAGPRPAVVLIGEPLFLVGWIALLYGFCFRVPVIADLIDLWPEADTAAPRGWRGALR
                     RAAYGGLAASRGLRLRRYKAVAFASRSYARLLAPKDANAPVFYWGSQLAPRRPRAAHS
                     EPIVAIYAGSLGLGYDIETVLAAAAIVRGANAPLRVAIAGDGPKREAVIRAQQAGVID
                     YLGQLSGGALIDAYEKADIGLLPYQSSSKVAMPIKFFDYLNFGLYIVSSLTMEAQELI
                     AERAIGASYLPGDAPDLAARLIEAAANRARLARAQAASKALAAEFAVGAQYRRFARFV
                     LLPREAGEGNRPKGGGGGPGG"
     gene            complement(2483..3439)
                     /locus_tag="VKU03_07125"
     CDS             complement(2483..3439)
                     /locus_tag="VKU03_07125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HLJ71071.1"
                     /translation="MTYLRNEVSIVTGGAGFLGSHLCARLLKNGRQVLCVDNFFTGSK
                     RNIAALLADPNFELMRHDITFPLYVEAGQIYNFACPASPIHYQHDPVQTTKTSVHGAI
                     NMLGLAKRVGARILQASTSEVYGDPEVHPQTEAYWGRVNPIGLRSCYDEGKRCAETLF
                     FDYYRQHKLDIKVVRIFNTYGPNMHPNDGRVVSNFIVQALRGEPITVYGDGRQTRSFC
                     YVDDLIDAIVAMMNDSPAGFPGPVNIGNPGEYTMVELAEKVLALVGGKSKLVFAPLPE
                     DDPKQRQPDIALAKKMLGWSPKVPLEDGLKETVAYFRKLVGA"
     gene            3646..4254
                     /locus_tag="VKU03_07130"
     CDS             3646..4254
                     /locus_tag="VKU03_07130"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020085454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLJ71072.1"
                     /translation="MKRAFDIFVSALALALGAPLFAILALLVRIKLGAPVFFVQMRPG
                     LRGALFPLRKFRSMTDARGADGALLDDARRLTPFGRWLRATSLDELPELWSVLTGDMS
                     LVGPRPLLKDYLPLYSAEQARRHDVRPGLTGWAQVNGRNALSWEEKFRLDLWYVDHQS
                     FLLDLKILAMTFGKILRREGIAHDGEATMPRFLGSAGETERP"
     gene            4251..4898
                     /locus_tag="VKU03_07135"
     CDS             4251..4898
                     /locus_tag="VKU03_07135"
                     /inference="COORDINATES: protein motif:HMM:TIGR03570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyltransferase"
                     /protein_id="HLJ71073.1"
                     /translation="MTAPRLLILGAGGHGKVAADCARAHGEWPAIEFFDDRWPSLSAC
                     ADWPVVGTGADLLRRDRDGAAFVAIGDAATRLDWIARLRAAGFELATIVHPRAIVSPG
                     AAIGPASLVVAGAVVNIGARLEEGVIVNTGATVDHDCALAAGAHICPGAHLAGGVEIG
                     RGAWIGVGAALRQGIRIGAGATIGAGAAVVQDIAEGVTAHGVPARAVADGTKNGR"
     gene            4900..6108
                     /locus_tag="VKU03_07140"
     CDS             4900..6108
                     /locus_tag="VKU03_07140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013214236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS aminotransferase family
                     protein"
                     /protein_id="HLJ71074.1"
                     /translation="MLDTPFSPWPSFSEEEIEAVSRVLRSGRVNYWTGGEAKAFEEEF
                     AEWCGARHAIALANGTLALEAALRALKIGPGDEVVVPARTFLATASAAIQCGAVPVCA
                     DIDPDSQNVTAETIAAVLTPRTKAVICVHLAGWPCDMDPILALCDARGLALVEDCAQA
                     HGARYKGRSVGTIGHIGCWSFCQDKIITTGGEGGMVTTNRRDWFEAMWAFKDHGKSWR
                     AVHAREHPPGFRWLHESFGSNYRMTEMQAAIGRAQLRRLPEWSKARRGNAERIWRAAG
                     QIQALRAPTPSDGFEHAAYKAYVFVRPEALAEGWTRDRIVAAIEAAGAPCYTGSCSEI
                     YLEKAFTEAGLGPKQRLPAAKALGEESMMFLVHPTLRDDEIAKTCAALQSVMAQAARG
                     GTSARTGTEG"
     gene            6105..6776
                     /gene="rpe"
                     /locus_tag="VKU03_07145"
     CDS             6105..6776
                     /gene="rpe"
                     /locus_tag="VKU03_07145"
                     /EC_number="5.1.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019995925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004750 - D-ribulose-phosphate 3-epimerase
                     activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribulose-phosphate 3-epimerase"
                     /protein_id="HLJ71075.1"
                     /translation="MTRAVKIAPSILAADFSRLGDEVRAADKGGCDWIHVDVMDGHFV
                     PNITFGPLAVAAARAATKKPIDVHLMIAPADPYLETFAKAGADRIIVHAEAGPHLDRS
                     LQTIRGLGKKAGVAINPATPESAIAYVLDRIDTILVMTVNPGFGGQTFIPAMLDKIRR
                     VRAMIGDLPIELEVDGGVAATNASEIAAAGANVFVAGSSIFESGDYAKAIAALRAAAT
                     AGGSR"
     gene            complement(6821..7168)
                     /locus_tag="VKU03_07150"
     CDS             complement(6821..7168)
                     /locus_tag="VKU03_07150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c family protein"
                     /protein_id="HLJ71076.1"
                     /translation="MASRPAAAEDAAAGERVFLKCRACHQIGEGAHNSVGPVLNGVVG
                     RKAGTYPGYNYSEANKNSGLVWDEATLKEYLKNPQAKVPGAKMTFPGLSREKDIDDVI
                     AYLKTFGPDGKKL"
     gene            complement(7323..8075)
                     /locus_tag="VKU03_07155"
     CDS             complement(7323..8075)
                     /locus_tag="VKU03_07155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HLJ71077.1"
                     /translation="MAKRSLVATLFWPGLFTALGLALLIALGVWQLHRLAWKEGLIAE
                     VSARVGAPPVALPPESAWPSLKPADYEYRHVRAAGSYDFAHQALIFRALGNPRGRYGG
                     PGYLVLTPLKLDDGAIVIVNRGFVPEDKKAAFSGGADQGAPAAEITGLMRSPESRTWF
                     TPADDPAAGQWFTRDPAAIAAAMKLARVAPFTIDADAGADPAALPEGGETILSFPNNH
                     LSYAFTWFGMALTLIGVFGAFAWKKREEFARE"
     gene            complement(8081..8455)
                     /locus_tag="VKU03_07160"
     CDS             complement(8081..8455)
                     /locus_tag="VKU03_07160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266729.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF983 domain-containing protein"
                     /protein_id="HLJ71078.1"
                     /translation="MNEAEHAYASSFAMGLAGRCPSCGKGRLFDGFVALAPRCAACGL
                     DYSFADSGDGPAVFVALIGGFIVLGAALWTEIVYEPPIWVHMVVFLPLTIVVCLGLLR
                     PFKGVLIALQYRNKAAEGRPEG"
     gene            complement(8507..>9020)
                     /locus_tag="VKU03_07165"
     CDS             complement(8507..>9020)
                     /locus_tag="VKU03_07165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HLJ71079.1"
                     /translation="IQYSRVAFTGGMWPPKGTEVLDPLNLPLLNTLILLTSGTTVTWA
                     HHALLHNDRKGLKIGLWLTILLGATFTCVQAWEYIHAPFNFKNTIYGATFFMATGFHG
                     AHVIIGTIFLSVCLVRAYWGQFTPKQHLGFEFAAWYWHFVDVVWLFLFSCIYVWGSWG
                     ATIEGATGGG"
BASE COUNT         1533 a         2935 c         3082 g         1470 t
ORIGIN      
        1 ccgcggcatc agcacggcgg ctgataaagg cgcgctacgc cccctctccc gcttgcggga
       61 gagggctggg gtgagggtaa gcggcggcgc ggcaagcatc gaaccggctg acgctttcca
      121 actggcggac tgagtcttag aaggccgatc gtcccgttgg ggacgctgtt ggcgaggata
      181 ttacgtggcg cgggccggca agacagacgc cattctggtc atcaagctcg gctcgctcgg
      241 cgatttcgtc cagtgcttcg acgcgttcca cgatattcgc gcccatcatt ccggcgcgcg
      301 gatcgcgcta ttgacgacgc cgcctttcgc ggcgctggcg cggcgctcgc cctggttcga
      361 cgaagtctgg acggacgggc ggcccgattg gaccgatctt tcggcatggg cgcgcctgat
      421 cttcaggctg cggcgggcgc gcttcgccag ggtctatgac ctgcaatata accgccgctc
      481 cgcgttcatt tttcgcgcgc tcggcggcgc caagggcccg ctttggctcg gcaaggcgaa
      541 aggctgcgcc attcccgagc cgctttatcc ggccgaagcc ggcaatgccg agcgcctcag
      601 gctgcatttg caaagcgccg gcgcgccctc ggccggcgag ccggaccttt cctggctggc
      661 cgacgacatt gccggcctcg cgcctcccga tcctttcgtg ctgttcgtcc cgggctgctc
      721 gccgcatttg ccgcagaagc gctggccggc ggagaattac gcggcgctcg gccgccggct
      781 ggcggcgcgc ggctatggcg tcgcgctggc cggcacgaag gccgatcgcg aggcggtcga
      841 ggcgattcgc gcgcgtttcg agggcgcgat cgatctttgc ggacggacca atctcttcca
      901 attggcgagc ctggcgcgcg cggccaaggc ggtggtcggc aacgacaccg ggccaatgtt
      961 ccttgccgcc aaaatcggcg cgccgacgtt gacgctgatg tcggcccata ccgacgagaa
     1021 gcgctccgcg ccgcccgggc cggccgcgtc ctggctcaag cgcgacgatc ttgcggcgct
     1081 cggcgtcgac gaggtcgaga gcgcgctgaa cttgccggcg cgaaatcctc ccccgtcgaa
     1141 gacgggggag ggggaccgcg cgtaagcgcg gtggaggggg ctccagtcta tcagaacaat
     1201 tctcaaccgc cggggccccc tccaccgccc ttcgggcggt tcccctcccc cgcttcgcgg
     1261 gggaggagta cgaagcgggc gaagcgccga tattgcgccc ccaccgcgaa ttccgccgcg
     1321 agcgccttcg aggcggcttg cgcgcgcgcg aggcgcgcgc gattcgctgc ggcttcgatc
     1381 agccgcgccg ccaggtccgg cgcgtcgccc ggcaggtacg aggcgccgat cgccctttcc
     1441 gcgataagct cctgcgcctc catcgtgagg ctcgagacga tatagaggcc gaaattcaga
     1501 tagtcgaaga acttgatcgg catcgcgacc ttgctgctgg actggtacgg caggaggccg
     1561 atatcggctt tctcgtacgc gtcgatcaat gcgccgccgc tcaattggcc gagatagtcg
     1621 atcacgcccg cctgctgcgc ccggatcacc gcttcgcgct tcggcccgtc gccggcgatc
     1681 gcgacgcgca gcggcgcgtt tgcgccgcgg acgatcgccg ccgccgccag cacagtctcg
     1741 atgtcgtagc caaggcccag cgagccggca tagatcgcga cgatcggctc ggaatgagcg
     1801 gcgcgcggac ggcgcggcgc cagttgcgac ccccaataga agaccggcgc gttcgcatcc
     1861 ttcggcgcga gcagccgcgc ataggagcgc gaggcgaagg cgacggcttt gtagcggcgc
     1921 agcctcagcc cgcgcgaggc ggccagcccg ccataggcgg cgcgccgcag cgcgccgcgc
     1981 cagccgcgcg gcgccgccgt gtcggcttcc ggccaaagat cgatgagatc ggcgatgacc
     2041 gggacgcgaa agcaaaagcc gtagaggagc gcgatccagc cgaccaggaa caacggctcg
     2101 ccgatgagga cgacggccgg gcgcgggccg gcggcgatgc gcacaaaggc cttcagcccg
     2161 aaccaggcct catgcgcgat ccgggcgagc gagagattgc gccggtaagc gaacgagcgg
     2221 atttgcctca gatagggaag gcgccgctgg ttgagcgcga tcgccgcggc cgagcgcctt
     2281 tgcttggaat tgtgcacgaa atccgaacag aagaattccg gcgccgcgtc aagcgctatg
     2341 agctgctcgc agagattgac atagcggtca ttggcgccgc cttcggacgg caaggcggca
     2401 tagggacgga tgacggcgac gcgccgcggc ctcgcctcgt ccattcagcg gcgctcgtat
     2461 gttgaggcgg cgtccgcgaa actcacgcgc cgaccagctt gcggaaatag gcgaccgtct
     2521 ccttgagacc gtcttcgagc ggcactttcg gcgaccagcc gagcatcttc ttggcgagcg
     2581 cgatgtcggg ctggcgctgc ttggggtcgt cttccggcaa gggcgcgaag acgagcttcg
     2641 acttgccgcc gaccaaggcc agaaccttct cggcgagctc gaccatcgta tattcgccgg
     2701 gattgccgat attgaccggc ccggggaaac cggcgggcga atcgttcatc atcgcaacga
     2761 tcgcgtcgat aagatcatcg acatagcaga acgagcgggt ctggcggccg tcgccataga
     2821 cggtgatcgg ctcgccgcgc aaagcctgga cgatgaaatt gctgaccacg cgcccgtcat
     2881 tgggatgcat gttcggcccg tacgtgttga agatgcgcac gaccttgatg tcgagcttgt
     2941 gctgccggta atagtcgaag aacagggtct cggcgcagcg cttgccctcg tcgtagcaac
     3001 tgcgcaggcc gatcgggttg acccggcccc aataggcttc ggtctgcgga tggacctccg
     3061 ggtcgccgta aacttccgaa gtggaggcct gcaagatgcg cgcgccgacc ctcttggcga
     3121 ggccgagcat attgatcgcg ccatggacgc tggtcttggt cgtctggacg ggatcgtgct
     3181 gatagtggat cgggcttgcc gggcaggcga aattgtagat ttgccccgcc tcgacataga
     3241 gcggaaacgt gatgtcgtgg cgcatcagct cgaaattggg atcggcgagc aaggccgcga
     3301 tattgcgctt cgagccggtg aagaaattgt cgacgcaaag cacctgccgg ccgtttttga
     3361 gcagccgcgc gcaaaggtgc gagccgagaa accccgcccc gccggtgacg atcgagactt
     3421 cgttgcgcag ataagtcatg aattcccctt cggccgccgg cgcggcccgg cggcgctgag
     3481 cgatgcgtcc gggattaagg cgcaaggcgc ggaaaaatcc agcttaaatc gccttgccgg
     3541 ggccggcccg ctggatcatc cacgacttgc gcggcccccg gattcggctt atcggctggc
     3601 ggcgcctcgg ccttcaggcg cccatcgaac gagcgctcaa gcgacgtgaa gcgggctttc
     3661 gacattttcg tctccgcgct ggccctcgcc ctcggcgcgc cgctttttgc gattctcgcg
     3721 ctcctggtcc gcatcaagct cggcgcgccg gtcttcttcg tgcagatgcg gcccggcttg
     3781 cgcggcgcgc ttttcccttt gcgcaaattc cgctcgatga ccgacgcgcg cggcgctgac
     3841 ggcgccctgc tcgacgatgc gcggcgcttg acgcctttcg gccgctggct gcgcgccacg
     3901 agcctggacg aactgccgga attgtggagc gtgttgaccg gcgacatgag cctggtcggg
     3961 ccgcggccgc tgctgaagga ctatcttccg ctctacagcg ccgagcaggc gcgccgccat
     4021 gacgtgaggc ccggcctcac cggctgggcg caggtcaacg gccgcaacgc tttgtcctgg
     4081 gaggagaagt tccggctcga cctatggtac gtcgatcacc agtcattcct tctcgacctc
     4141 aagattctcg ccatgacgtt cggcaaaatc ttgaggcgcg aggggatcgc acatgacggc
     4201 gaagcgacga tgccgcgctt cctgggctcc gccggcgaga cggagcggcc atgaccgcgc
     4261 cgcggctgtt gattctcggc gctggcggcc acggcaaagt cgcggctgat tgcgccaggg
     4321 cgcacggcga atggccggcg atcgaattct ttgacgaccg ctggccgtcg ctgtcggcct
     4381 gcgcggattg gccggtcgtc gggaccggcg cggatctgtt gcgccgggac cgggacggcg
     4441 cggcttttgt cgcgatcggc gacgccgcaa cgcggctcga ctggatcgcg cggctccgcg
     4501 cggcgggctt tgaactggcg acgatcgtcc atccacgcgc gatcgtcagt cccggcgccg
     4561 cgatcggacc ggcgagcctc gtcgtcgccg gcgccgtcgt caatatcggc gcccggctcg
     4621 aagagggcgt catcgtcaat accggcgcca cggtcgacca tgattgcgcg ctcgccgccg
     4681 gcgcgcatat ctgtcccggc gcacatctcg cgggcggcgt cgagatcggc cgcggcgcct
     4741 ggatcggcgt cggcgcggct ctgcggcagg ggatcaggat cggcgccggc gcgacgatcg
     4801 gggccggcgc cgcggtggtt caggacatcg ccgagggcgt caccgcccat ggcgtgccgg
     4861 cgagagccgt cgccgacggt acgaagaacg gacgctgaaa tgctggacac gcctttctcg
     4921 ccctggccga gcttttccga ggaggagatc gaggcggtgt cgcgcgtgct tcgctccggg
     4981 cgcgtcaatt actggaccgg cggcgaggcc aaggcgttcg aggaggagtt cgccgagtgg
     5041 tgcggcgcgc gccacgccat cgccctcgcc aacgggacgc tggcgctcga agccgcgctc
     5101 cgcgccctga agatcggccc cggcgacgag gtcgtcgtgc ccgcgcgcac gtttctcgcc
     5161 acggcctcgg cggcgatcca atgcggcgcc gtcccggtct gcgccgacat cgatccggat
     5221 tcgcagaatg tcaccgccga gacgatcgcc gcggttctga cgccgcggac caaggccgtc
     5281 atctgcgtcc atctcgccgg ctggccttgc gacatggacc cgatcctggc gctgtgcgat
     5341 gcgcgcgggc tcgcccttgt cgaggattgc gcgcaggcgc atggcgcccg ctacaagggg
     5401 cgctcggtcg ggacgattgg ccatatcggc tgctggtcct tctgccagga caagatcatc
     5461 acgaccggcg gcgagggcgg catggtgacg accaaccgcc gcgactggtt cgaagcgatg
     5521 tgggcgttca aggaccacgg caagtcgtgg cgggccgtgc acgcgcgcga acatccgccc
     5581 ggctttcgct ggctgcatga gtccttcggc agcaattacc gcatgaccga gatgcaggcg
     5641 gcgatcggcc gcgcgcaatt gcgccgactg ccggaatgga gcaaggcgcg gcgcggcaac
     5701 gcggagcgga tttggcgtgc tgcgggacag attcaagcgc tgcgcgcgcc gacgccgtcg
     5761 gatggattcg agcatgcggc ctataaggcc tatgtcttcg tgcggcccga agcgctcgcc
     5821 gaaggctgga cgcgcgaccg catcgttgcg gcgatcgaag cggcgggcgc gccctgctat
     5881 acgggctcct gctcggagat ttatctggaa aaagccttta ccgaggccgg ccttgggccg
     5941 aaacagcggc tgccggcggc caaggcgctg ggcgaggaga gcatgatgtt cctggtccat
     6001 ccgaccttgc gcgacgacga aatcgccaag acctgcgcgg cgctgcaaag cgtgatggcg
     6061 caagcggcgc gaggcgggac aagcgcgcgg acggggacag agggatgacc agagccgtca
     6121 agatcgcccc gtccatcctc gccgcggatt tctcccggct cggtgacgag gtccgggccg
     6181 ccgacaaggg cggctgcgac tggatccatg tcgatgtgat ggacggccat ttcgtgccga
     6241 acatcacctt cgggccgctc gccgtcgcgg cggcgcgcgc cgcgaccaag aagccgatcg
     6301 atgtccatct gatgatcgcg ccggccgatc cgtacctcga aactttcgcc aaagccgggg
     6361 cggaccgcat catcgtccat gccgaggccg ggccgcatct cgaccgctcg ctacagacaa
     6421 tccgcggcct cggcaagaag gccggcgtcg cgatcaatcc ggcgacgccg gaaagcgcca
     6481 tcgcctatgt cctcgaccgc atcgacacga tcctcgtgat gacggtcaat ccgggctttg
     6541 gcggccagac cttcatcccg gccatgctcg acaagatcag gcgggtcagg gcgatgatcg
     6601 gcgatttgcc gatcgagctt gaggtcgatg gcggcgtcgc ggccacgaac gcgtccgaga
     6661 tcgccgcagc gggcgccaat gtcttcgtcg ccggatcgtc gattttcgaa agcggcgact
     6721 acgcgaaagc gatcgccgct ttgcgcgcgg cggcgacggc cggcggctcg cgatgatttt
     6781 cgacgagcgc atttgagcgg ccagagcgag cgccgaagcg ctaaagcttc ttcccgtcgg
     6841 gcccaaacgt cttcagatag gcgataacgt cgtcaatgtc tttttctcgc gacaggccgg
     6901 ggaaggtcat cttggcgccg ggaaccttcg cttgcggatt cttgaggtac tctttcaacg
     6961 tcgcctcgtc ccaaacgagg cctgaattct tgttggcttc gctgtagttg tagccggggt
     7021 aggttcccgc tttgcggccg accacgccgt tcagaaccgg gccgacgcta ttatgcgcgc
     7081 cttcgccgat ctgatggcag gccctgcatt tgaggaagac tctttcgccg gccgcggcgt
     7141 cctcggcggc ggcgggacga gaggccatca gcgatgtcga gagagcgatg gcgatgctca
     7201 aaacttggca ggcgaaacga ctcaatcttc tcctcccctc ggccgaatgc gcggggaagg
     7261 atcatgtcag actcggcccg gcgccgtcac gcggcgcgtg gtcgcggctc gttcggcgcc
     7321 cttcattctc tcgcgaactc ttcgcgcttc ttccaggcga aggccccgaa aacgccgatc
     7381 aaggtcaacg ccatgccaaa ccaggtgaag gcgtaggaaa gatgattgtt gggaaaggaa
     7441 aggatcgtct cgccgccttc gggcaaagcg gccggatcgg cgccggcgtc ggcgtcgatc
     7501 gtgaacggcg cgacgcgggc aagtttcatc gccgcggcaa tggcggcggg atcgcgggtg
     7561 aaccattggc cggccgccgg atcgtcggcg ggcgtaaacc aggtgcgcga ttcgggcgag
     7621 cgcatcagcc cagtgatttc ggcggctggc gcgccctggt ccgcgccgcc ggaaaaggcg
     7681 gctttcttgt cctctgggac aaagccgcga ttgacgatga cgatcgcgcc gtcgtcgagc
     7741 ttgagcggcg tcagcaccag ataacccggg ccgccatagc ggccgcgcgg atttccgagg
     7801 gcgcggaaaa tcagcgcctg atgggcgaaa tcgtaggaac cggcggcgcg cacatgccga
     7861 tattcatagt ccgccggctt tagactcggc caggcggatt ccggcggcaa ggcgaccggc
     7921 ggcgcgccga cccgcgccga aacttcggcg attaaaccct ctttccaggc gagccgatgc
     7981 aattgccaga cgccgagcgc gatcagcaaa gcgaggccga gggccgtgaa taggccaggc
     8041 caaaacagcg tcgcgacaag gctgcgcttc gccaccggtt tcagccttcc ggccggcctt
     8101 cggcggcctt gttgcggtat tgcagggcga tcagcacgcc tttgaacggc cgcagcaggc
     8161 cgaggcagac gacgatcgtc agcggcagga acacgaccat atggacccag atcggcggct
     8221 catagacgat ttccgtccac agagccgcgc cgagcacgat gaagccgccg atcaaagcga
     8281 cgaagaccgc cgggccgtcg cccgaatcgg cgaaggaata atcgaggccg caggccgcgc
     8341 agcgcggcgc gagcgcgacg aagccgtcga acagccggcc cttgccgcag ctcggacatc
     8401 gcccggccag acccatcgca aaagacgaag cgtaagcgtg ttcggcttcg ttcattcaag
     8461 accgccgagg cgttctctgg ctcggcgcag aaaagagcgg agcgcctcag cctccgcccg
     8521 tcgcgccttc gatcgtcgcg ccccacgagc cccacacata gatgcaggaa aacaggaaca
     8581 gccagacgac gtcgacgaag tgccaatacc aggcggcgaa ctcgaagccg agatgctgct
     8641 tcggcgtgaa ctggccccaa taggcgcgga ccaggcaaac gctcaggaag atcgtcccaa
     8701 taatcacatg ggcgccatgg aagccggtcg ccatgaaaaa ggtcgcgcca tagatcgtgt
     8761 tcttaaagtt gaacggcgcg tggatatatt cccaggcctg aacgcaggtg aaggtcgcgc
     8821 cgagcaggat cgtcagccac aagccgattt tgaggccctt gcggtcgttg tgaagaagcg
     8881 cgtgatgggc ccaggtgacg gtcgtgcccg aggtcagcag gatcagcgtg ttgagcagcg
     8941 gcaggttcag cggatcgagg acttccgtgc ctttgggcgg ccacatgccg ccggtgaagg
     9001 cgacgcgcga atattggatc
//
LOCUS       DATGJW010000228         3128 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1180727, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000228 DATGJW010000000
VERSION     DATGJW010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3128)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3128)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3128
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1180727"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(199..2022)
                     /gene="glmS"
                     /locus_tag="VKU03_07170"
     CDS             complement(199..2022)
                     /gene="glmS"
                     /locus_tag="VKU03_07170"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015824273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HLJ71080.1"
                     /translation="MCGIVGILGAKPVAGQILDALKRLEYRGYDSAGIATLEQGRLTR
                     RRAEGKLRNLEARLSKEPLSGAIGIGHTRWATHGKPIEANAHPHASKRLAVVHNGIIE
                     NFRELREELSARGHRFETETDTEVAAFLVTDELDQGKAPIQAVAAALPRMRGAYALAF
                     LFEGEDDLLIGARQGAPLAVGYGEGEMYVGSDALALAPFTGEIAYLEEGDWVVLTRSG
                     AEFHDASGRIVARKRQRSPAGSLLIEKGEYRHFMAKEIHEQPEVVGHALAQYLDLSRL
                     CVRPFAWPVEPKTLKRLTICACGTAYMAGLTAKYWIERFAKLPVDIDVASEYRYREAP
                     VEKDDLVIAISQSGETADTLASLRYAQERGAKIVAVVNVISSSIARLADAPAPIMAGP
                     EIGVASTKAFTCQLAVMAALALSLGRARGTLDEASEAALVKQLVAIPGLIAETLKHEP
                     ALEGLAHTLAKARDVLYLGRGLSYPLALEGALKLKELSYIHAEGYAAGELKHGPIALI
                     DEAMPVIVIAPHDAVFDKTVSNMQEVAARGGRIVLIGDKRAAEKAAVDLSYFFAMPDM
                     GPDFAPIVYAAPIQMIAYHTAVVMGKDADQPRNLAKSVTVE"
     gene            2206..>3128
                     /locus_tag="VKU03_07175"
     CDS             2206..>3128
                     /locus_tag="VKU03_07175"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020095544.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLJ71081.1"
                     /translation="MGRGFHRYGALALAASLWPLAAFAASRPAVEAAHGIVVSSQRLA
                     SEIGAEVLKEGGNAIDAAVAVGYAEAVTNPCCGNIGGGGFMVIHLAKDNRDVFINFRE
                     TAPAAATRTMYLDEKGQPIPGASLNGYRAVGVPGTVLGLDTARKEYGTWPRAKVMAPA
                     IKLARDGFVLTRADTDIIEAGAARLKRDPVAAKIFFRPDGSLLEPGDRLIQSDLAATL
                     QAIADQGADGFYKGGVAEKIDAAMQAHGGIMTAKDLADYKITETPPVTCTYRGYVFAS
                     SPPPSSGGATLCEILNILEGYDLRSLGFHSA"
BASE COUNT          549 a         1006 c         1039 g          534 t
ORIGIN      
        1 ggccggcgat aaggagctgc aggagctttc cgacgaattg ggcggtacgg tataagccgc
       61 cgccgtcatt gcgagcgaag caaagcaatc cgttcacgta ccgcggtcat tgcaaggagc
      121 gaagcgacga aacaatccat tcgtaagacg tcgccctatg gatggattgc ttcgctgcgc
      181 tcgcaatgat ggcgaatcct actccaccgt caccgacttc gccaaattgc gcggctgatc
      241 ggcgtctttg cccatgacga ccgcggtatg gtaggcgatc atctggatcg gcgcggcgta
      301 gacgatcggc gcgaaatccg gtcccatatc gggcatggcg aaaaagtacg aaaggtcgac
      361 cgccgccttc tcggcggcgc gcttgtcgcc gatcaggacg atccgtccgc cccgcgccgc
      421 gacttcctgc atgttggaga cggtcttgtc gaacaccgcg tcatgcggcg cgatgacgat
      481 gaccggcatc gcctcgtcga tcagcgcgat cggcccatgc ttgagttcgc cggcggcgta
      541 gccctcggcg tgaatatagg agagctcctt cagcttcaga gcgccttcca gcgccagcgg
      601 ataggaaagc ccgcggccga gatagagcac gtcgcgcgcc ttggcgagcg tatgggcaag
      661 gccttcgagc gccggctcgt gcttgagcgt ttcggcgatc aagccgggga tcgcgacgag
      721 ctgcttgacg agagccgcct cgctcgcttc gtccagcgtt ccgcgcgcac ggccgaggct
      781 gagcgccagc gcggccatca cggcgagctg gcaggtgaag gccttggtcg aggcgacgcc
      841 gatttccggc ccggccatga tcggcgccgg cgcgtcggcg agccgcgcga tgctcgacga
      901 gatcacattg acgacggcga cgatcttggc gccgcgctcc tgcgcgtagc gcaacgaggc
      961 tagcgtgtcg gcggtttcgc ccgactgcga aatggcgatg accagatcgt ccttttcgac
     1021 cggcgcctcg cgatagcgat attccgaggc gacgtcgatg tcgaccggca gcttggcgaa
     1081 gcgctcgatc caatatttgg cggtgaggcc cgccatatag gcggtgccgc aggcgcagat
     1141 cgtcagccgc ttcagggtct tcggctcgac cggccaggcg aagggccgca cgcagagccg
     1201 agataaatcg agatattgcg ccagcgcgtg gccgacgacc tccggctgct cgtggatctc
     1261 cttggccatg aaatggcggt attcgccctt ctcgatcagc aggctgccgg ccgggctgcg
     1321 ctggcgcttg cgcgcgacga tccgacccga ggcgtcgtgg aattcggcgc cggagcgggt
     1381 cagcacaacc cagtcgccct cttcgagata ggcgatctcg ccggtgaagg gcgccagcgc
     1441 caacgcgtcg gagccgacat acatctcgcc ctcgccatag ccgacggcga gcggcgcgcc
     1501 ttggcgcgcg ccgatcagga gatcgtcctc gccttcgaac agaaaggcca aagcataagc
     1561 gccgcgcatg cggggcaaag cggcggcgac cgcctggatc ggggccttgc cctgatcaag
     1621 ctcatcggtg accaggaacg cagcgacttc ggtgtcggtt tcggtctcaa agcgatggcc
     1681 ccgggcgctg agctcctcgc gcaactcgcg gaaattctcg ataatgccgt tatggaccac
     1741 ggcgaggcgc ttgctggcgt gcggatgggc gttggcctcg atcggcttgc catgggtcgc
     1801 ccagcgcgta tggccgatgc cgatcgcgcc ggataatggt tctttcgaga gccgcgcttc
     1861 gagattgcgc agcttgccct cggcgcggcg gcgggtcagc cggccctgct ccagggtggc
     1921 gattccggcc gaatcgtagc cgcgatattc gaggcgcttc agcgcgtcga ggatctgccc
     1981 ggcgacgggc ttggcgccga gaatgccgac aatgccgcac atcgaggcct tgtccccttt
     2041 tcaaactgtg attccaggca gcaagagcta gaccaaggtc taatacccgc caagtcaagt
     2101 tggatgaccg aatatgaaac gttccaagca tagcacgcgc ggcgctggcg cgcttgccga
     2161 ggtcttggca tgatccgccg cgccagcgcc gaagggaggg gacaaatggg ccgagggttt
     2221 caccgatatg gcgcgctggc gctggccgct tccctttggc cgctggccgc ttttgccgcg
     2281 tcgcggccgg cggtcgaggc ggcgcatggg atcgtcgtct cctcgcagcg cctcgcctcc
     2341 gagatcggcg cggaagtgct gaaagagggc ggcaatgcga tcgacgcggc cgtcgcggtc
     2401 ggctacgccg aggcggtgac caatccgtgc tgcggcaata tcggcggcgg cggcttcatg
     2461 gtgatccatc ttgccaagga caatcgcgac gtcttcatca atttccgcga aacagcgccg
     2521 gccgcggcga ccaggacgat gtatctcgac gagaagggcc agccgatccc cggcgcgagc
     2581 ctgaacggct atcgcgcggt cggcgtcccg ggcacggtgc tgggcctcga tacggcgcgg
     2641 aaggaatacg ggacatggcc gcgagcgaaa gtcatggcgc cggcgatcaa gctcgcccgc
     2701 gacggtttcg tcctcacgcg cgcagatacc gacattatcg aagccggcgc ggcgcgcctg
     2761 aaacgcgatc cggtcgccgc gaaaatcttc tttcgccccg acggctcgct tttggagcca
     2821 ggcgaccgcc tgatccagag cgacctcgcc gcgacgctcc aggcgatcgc cgaccagggc
     2881 gccgacgggt tttacaaagg cggagtcgcg gagaagatcg atgcggcgat gcaggcgcat
     2941 ggcgggatta tgaccgccaa ggacctcgcc gactataaga tcactgaaac gccgccggtg
     3001 acttgtacct atcgcggcta tgtcttcgcc tcctcgcccc caccctcttc cggcggcgcc
     3061 accttgtgcg agatcctcaa catcctcgaa ggctacgatc tgcgcagcct cggcttccat
     3121 tcggcgga
//
LOCUS       DATGJW010000229         3449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_456892, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000229 DATGJW010000000
VERSION     DATGJW010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3449
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_456892"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..371)
                     /locus_tag="VKU03_07180"
     CDS             complement(<1..371)
                     /locus_tag="VKU03_07180"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLJ71082.1"
                     /translation="MPHIVAIGEPLYELNQPRGAEHFIPGFGGDTSNAMIAAARSGAD
                     TGYFTAIGADRFGEALVALWAREGVDSSRVVVNGGAHTGLYFVNHGPNGHEFSYLRAG
                     SAASRLTEADIPSDYVASARIL"
     gene            complement(458..1060)
                     /locus_tag="VKU03_07185"
     CDS             complement(458..1060)
                     /locus_tag="VKU03_07185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YqgE/AlgH family protein"
                     /protein_id="HLJ71083.1"
                     /translation="MARASEKSAGYLDGQMLIAMPALQDGPFARSLVYLCAHREDGAM
                     GIIVNQSAPAVKFPELLVQLGVVNESEAIVLPPRAGAIKVLRGGPVETGRGFVLHSSD
                     YFIDNSTLPIADDICLTATLDILKAIAKGDGPQKAVLALGYAGWSSGQLEAEIRNNGW
                     LHCPADPGLIFDSDFASKYERALAKIGASLGTLSGEAGRA"
     gene            1169..1996
                     /locus_tag="VKU03_07190"
     CDS             1169..1996
                     /locus_tag="VKU03_07190"
                     /inference="COORDINATES: protein motif:HMM:NF022853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-disulfide reductase DsbD
                     domain-containing protein"
                     /protein_id="HLJ71084.1"
                     /translation="MLAFRALFCAGALCLAAPAFAADTFSSDWAAGLKSSARLILGDA
                     ENGRFHAGIEIKLAPGAITYWRNPGDAGLPPTLNFDGSANLADARAFFPAPQRLNEGD
                     GAAFGYDHSVILPLDVQAADPGKPVTLALKLDYAVCEKICVPARAELRLRLAKDEGAS
                     PYAAAVAKARAEVPRPVDWAKLAGKATLASAGDKAWRLCFASPQPSARQLFVEAPPPW
                     WFAVAREAASPQDQSCFRVTLEQKPEDQNLPVAARFTVTGGDRAFETTATLGAPASQ"
     gene            2075..2560
                     /locus_tag="VKU03_07195"
     CDS             2075..2560
                     /locus_tag="VKU03_07195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peroxiredoxin"
                     /protein_id="HLJ71085.1"
                     /translation="MTIKVGDRLPNATFRLISEDGPLPVAAKDFFSGKKVVLFGLPGA
                     FTPTCHKNHLPGFLAHEEAFKEKGVDEIAMTSVNDHFVLAAWSKATGADGKIKFLADG
                     NGEFAKAAGLDFDATGGGLGLRSKRYSMLVEDGVVKKLNIEEAPGKVDLSGAERLLAE
                     I"
     gene            complement(2999..3286)
                     /locus_tag="VKU03_07200"
     CDS             complement(2999..3286)
                     /locus_tag="VKU03_07200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71086.1"
                     /translation="MTRHSQLDLFGESQPELPGVEGAPATYRPDTNEVRAELLRVLAK
                     VRAAQSFPWDARRTLYWRTVFPQMTNWLPDDEAAQLRFEFETEIRRLDAAL"
BASE COUNT          565 a         1160 c         1134 g          590 t
ORIGIN      
        1 aggattcgcg ccgaggcgac atagtcggaa ggaatatccg cttcggtgag gcggctcgcc
       61 gccgatccgg cgcgcaaata ggaaaattca tggccgttcg gcccgtggtt gacgaaatag
      121 agtccggtat gggcgccgcc attgaccacg acccggctcg aatcgacgcc ttcgcgcgcc
      181 caaagcgcaa ccaaagcctc gccgaagcgg tcggcgccga tcgcggtgaa atagcccgta
      241 tcggcgccgg agcgcgccgc ggcgatcatc gcgttcgacg tatcgccgcc aaagcccgga
      301 atgaaatgct ccgcgccgcg cggctggttc agttcataga gcggctcgcc gatggcgacg
      361 atgtgcggca tgcgagacct tgcgctgatt gaagcggacg cctcgcccgc cgcgccggcc
      421 ttgtcaagaa ctcaaaggcc gcgccctcgg cgcattctca agcccgcccg gcttcgccgg
      481 aaagcgttcc gagactggcg ccgatcttcg ccaaggcgcg ctcgtatttc gaggcgaagt
      541 cgctgtcgaa aatcaggccc ggatcggccg gacagtgcag ccagccgttg ttgcggattt
      601 ccgcttcgag ctgtcccgac gaccagccgg catagccgag cgccagcacc gccttttgcg
      661 gaccgtcgcc cttggcgatc gctttcagga tgtcgagcgt cgcggtgagg cagatgtcgt
      721 cggcgatcgg caaagtcgaa ttgtcgatga aatagtcgct cgaatgcaga acgaagccgc
      781 gcccggtctc gaccgggccg ccgcgcagca ccttgatcgc gccggcgcgc ggcggcagca
      841 cgatcgcctc gctctcgttg acgacgccga gctggaccag cagttcggga aatttcaccg
      901 ctggcgcgct ctggttgacg atgatcccca tcgccccgtc ctcgcgatgg gcgcagagat
      961 aaacgagcga gcgcgcgaac gggccgtcct gcaaggccgg catggcgatg agcatctgcc
     1021 cgtcgagata gccggcagat ttttccgaag cgcgcgccat ggacgcaatg ctacgcgcct
     1081 cgggcggcaa gtcaaagccg gcgcggacag ggccccctct cacgcctttg tgtaggccgc
     1141 tggccgccgc tcaggctata gggaaagcat gcttgccttt cgcgccctct tttgcgccgg
     1201 cgcgctctgc ctcgcggcgc cggctttcgc cgccgacacg ttttcgtccg attgggctgc
     1261 cgggctcaaa tcctcggcgc gactgatcct cggcgacgcc gaaaacgggc gctttcacgc
     1321 cgggatcgag atcaagctcg cgccgggcgc gatcacctat tggcgcaatc cgggcgacgc
     1381 cggcctgccg ccgacgctca atttcgacgg ctcggccaat ctcgccgacg cccgcgcctt
     1441 cttcccggcg ccgcagcgac tcaacgaggg cgacggcgcg gcgttcggct acgaccattc
     1501 ggtgatcctg ccgctcgacg ttcaagccgc cgatccggga aagccggtga cgctcgcctt
     1561 gaagctcgat tacgccgtct gcgagaagat ttgcgttccg gccagggccg agttgcggct
     1621 tcgcctggcc aaggacgagg gcgcttcgcc ttatgcggcg gctgtggcga aggctcgcgc
     1681 cgaggtgccg cggccggtgg attgggcgaa gctcgccggc aaggcgacgc tcgcctcggc
     1741 gggcgacaag gcctggcgcc tgtgcttcgc ctcgccccag ccttcggcgc gccaattgtt
     1801 tgtcgaggcg ccgcccccgt ggtggttcgc ggtcgcccgc gaggccgctt cgccacagga
     1861 ccaaagctgc tttcgcgtga cgctcgagca gaagccggag gaccagaatc tgccggtggc
     1921 ggcgcggttc acggtgacgg gcggcgaccg cgcgttcgag acgacggcga cgctcggcgc
     1981 gccggcgagc caatgaacgg cgcgccactt gcggttgcgt cttttcgcga tagataggcc
     2041 gggcgcgaag gcgcgcccat tttaggaacg agccatgaca atcaaagtcg gggaccgtct
     2101 gcccaacgca acattccgcc tgatcagcga agacgggccg ctgcctgtcg ccgccaagga
     2161 tttcttcagc ggcaagaaag tcgtcctttt cggcctgccc ggcgctttca cgccgacctg
     2221 ccacaagaac catctgcccg gcttcctcgc ccacgaagag gcgttcaagg agaaaggcgt
     2281 cgacgagatc gcgatgacct cggtcaacga tcatttcgtt ctcgccgcct ggtccaaggc
     2341 gaccggggcc gacggcaaga tcaagttcct cgccgacggc aatggcgaat tcgccaaggc
     2401 ggccggcctc gatttcgacg cgaccggcgg cggcctcggc ctgcgctcga agcgctattc
     2461 gatgctggtc gaggacgggg tcgtcaagaa gctcaacatc gaggaggcgc ccggcaaggt
     2521 cgatctctcc ggcgccgagc gtctactggc ggagatttga gtcggggacg cggcgcgccg
     2581 tcgaggttgc acgctcgcgc gacggcggcg attctcgcgc gctttatcct gcatgccatc
     2641 gccgtgctcg gcgtcaatcc caagccgctt cgcggtcgcc tcgccacggc aaccccgtag
     2701 cacattcttc cgacattgag ccatggcgag gcgaggattg acccctccgc tcggcgatgg
     2761 ctttcttgct accgtgaatt ttggtgatga aaacggcccc gtgggccggt ttcatcacca
     2821 aagttcacgg tagccgacat gccgcgccgg acgatcgcag tcacccgtaa gggccgaagc
     2881 gaagcgtagg acccgccata gcgcccctcc ggcggctgta cttttgtacg agcgctcgtg
     2941 gcgctatggc gggttgacgg cgatcggccg gcgtggcgcc gcggccctcg ccttttgact
     3001 ataaggccgc gtccagccgc cggatttccg tctcgaactc gaagcgcaat tgcgcggcct
     3061 cgtcatccgg cagccagttc gtcatctggg gaaagaccgt gcgccagtag agcgttcggc
     3121 gcgcgtccca tggaaagctc tgcgccgcgc ggactttcgc gagcacgcgc aagagctcgg
     3181 cgcgcacttc gttcgtatcg gggcgatacg tcgcgggcgc tccttcgacg cccggaagct
     3241 ccggctggct ttcgccgaaa aggtccaatt gactgtggcg cgtcatggcg ggcgggttgt
     3301 cctcgtctcg cagcaatcag taggctgagt ttgccagaac ccgcgggcta gtgcgacggc
     3361 ccgtcgtccg ttgtccccga ctgttcgtcg gggctcacaa cgacaagcgg ccccgccacg
     3421 atccggtcga gcaggtccgc ctgccgcgg
//
LOCUS       DATGJW010000230         5915 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_873070, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000230 DATGJW010000000
VERSION     DATGJW010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5915)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5915)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5915
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_873070"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            64..594
                     /locus_tag="VKU03_07205"
     CDS             64..594
                     /locus_tag="VKU03_07205"
                     /EC_number="3.1.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455537.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetyl-ADP-ribose deacetylase"
                     /protein_id="HLJ71087.1"
                     /translation="MTTTPARLEVLVADITTLDVDAIVNAANQRLAGGGGVDGAIHRA
                     AGARLLEACRKLGGCPTGDAKITPGFNLKARHVIHAVGPVWGGGKSNEDALLASCYRR
                     ALELASEHRLASIAFSAISTGVYGFPAERAADIAVSAVGETLPQLVAPPARVIFCCFS
                     ERSAELHRAALRKRGE"
     gene            complement(683..2092)
                     /locus_tag="VKU03_07210"
     CDS             complement(683..2092)
                     /locus_tag="VKU03_07210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003530079.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5939 domain-containing protein"
                     /protein_id="HLJ71088.1"
                     /translation="MAEIDTLWTLLAQSADAEVAASLKSLVATGEDRALNRINALAYA
                     AERGHDEEATISTLIHAARLGLFDLSWNVLCPGCGGVLETGSGLKTLDRHEYFCSLCA
                     GSYEPTLDELVEVTFTVNPRVRHIAAHDPETLPLGEYARQIFWGSGVDLPGDIDAAMN
                     ELTLDAMELAPGEKAAMSLTLPESFIIAFDPVTHTTLFMEVSGEPTHERRNVSLVFSD
                     LRAHAGKLVLQPGPARITFENRTSKRTIPGLWLAGEAMHRILGARRPFLTATRLLSNQ
                     TFRDLYRTGTLDKDQRFKITNLTILFTDLRGSTALYDRVGDLAAFDLVRDHFSALLNT
                     VAAEGGAVVKTIGDAVMATFPSPDRAVRAAMKMRGAMRQINEARGSEDLALNIGLHEG
                     PCLAVMLDERQDYFGQTVNVASRVQGLADPSAILVTKPIVEAANVAKILAEAGMAATE
                     RQAYTLRGISEPVPVYELR"
     gene            2286..2702
                     /locus_tag="VKU03_07215"
     CDS             2286..2702
                     /locus_tag="VKU03_07215"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLJ71089.1"
                     /translation="MKTIEAQCRCGAIGLKIAGEPVVQLYCHCGDCQAAHGAAYAPAT
                     IYPAPAVEVVRGEPAPMVVRTTQRMRCAACGTYLFSELASAGVRSVSAMLLPKGEFKP
                     QFHVQCEDAVLPVVDNLPHYKRFPAAFGGAETFVDW"
     gene            complement(2802..4073)
                     /locus_tag="VKU03_07220"
     CDS             complement(2802..4073)
                     /locus_tag="VKU03_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71090.1"
                     /translation="MRSRRAAIVLLGALAAGPVAADSDTPTWQGVYEGVIGKAHVVAA
                     LAPSGARYVYLGQANDLGLIVSDAGGALTMVETLAPGVDEDAAKEHPELVSGAWTARF
                     ADGALKGVWKDAHGGHERPLALTRISTDDESVGALTTGDRPGAYDERWLRGAPPVVAA
                     GAEASLGTVSYRPMRDPLFGGLVPRLTRAPAGADLAAVNAGLDRLQRAQRVEDRECLQ
                     AQRAAAAPNGAEALKNEEKGAKDSAAALTPTYATARLLTIEKSGMVFCGGAHPNLEVA
                     VYTFDLADGALIAGGDDADPLGPSGLGRALDIQAAKRRAAFDALWMSDLRARIAAGEK
                     AAPQNSDDDPHCGQVLLDALRSDGAAIAKAVYPTAQGLAFRFLAFPHAVEGVCSGGYP
                     FNPLVVPYAALKPFLKPNQTLLPSPAAPAGK"
     gene            complement(4108..4758)
                     /locus_tag="VKU03_07225"
     CDS             complement(4108..4758)
                     /locus_tag="VKU03_07225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HLJ71091.1"
                     /translation="MRAERAGFWRKTPLVAVFAIAIAAAGFAVLYGKGASGKDEAALC
                     PKAAAIAQRLTPLAKGEVAALSVDKTPKPAIDVSFKDPDGKKLTLADFRGRTVLLNLW
                     ATWCAPCRSEMPALDRLEAKFGGPDFQVVAVNIDTTRLDKPKAFFADNGVKNLSLYAD
                     PTADSFEALKVAGKALGLPTTLLIDKEGCEVGVMAGPAAWDSKDAEAAVSALLGRG"
     gene            4779..>5915
                     /gene="argH"
                     /locus_tag="VKU03_07230"
     CDS             4779..>5915
                     /gene="argH"
                     /locus_tag="VKU03_07230"
                     /EC_number="4.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007567633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004056 - argininosuccinate lyase activity
                     [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate lyase"
                     /protein_id="HLJ71092.1"
                     /translation="MSNKMWGGRFEAGPSKIMEEINASIDFDRKLAAQDIAGSKAHIA
                     MLAETGIVAKADAAAIAKGLDKVGAEIEAGRFAFSRALEDIHMNVEARLAELVGPAAG
                     RLHTARSRNDQVALDLKLWVRDAIDTLDRQVAGLQQALAERALEHAGAVMPGFTHLQS
                     AQPVTFGHHLLAYVEMLARDRGRFADARKRLNECPLGAAALAGTSFPIDRAMTAKALG
                     FDRPTANSLDSVADRDFALEALAAAAIAAAHLSRFAEEIVLWTTPQFGFVRLSDAFST
                     GSSIMPQKRNPDAAELARGKTGRIFGALVGLLTVMKGLPLAYAKDMQEDKEGVFDALQ
                     TLSLVVAAMTGMVRDMTPDAKRMKAAAGAGYATATDLADWLVRRL"
BASE COUNT          953 a         1987 c         2003 g          972 t
ORIGIN      
        1 gtcgggcgag ctcgtcgccc atctcgccga ggcgggaatc gaggtcaagc agccggcgaa
       61 ggcctgacga ccacgcccgc gcgcctcgaa gtcctcgtcg ccgacatcac cacgctcgac
      121 gtcgacgcga tcgtcaacgc cgccaatcag cggcttgccg gcggcggcgg cgtcgacggg
      181 gccattcatc gcgccgccgg tgcgcgcctg ctcgaagctt gccgcaagct cggcggctgt
      241 ccgaccggcg acgccaagat cacgcccggc ttcaatctga aggcgcgcca tgtcatccac
      301 gccgtcggac cggtttgggg cggcggcaag agcaacgagg acgcgctgct cgcgtcctgc
      361 tatcgccgcg cgctggagct cgcctcggag catcgcctcg ccagcatcgc cttttcggcg
      421 atctcgaccg gcgtctacgg ctttcccgcc gagcgcgccg ccgatatcgc ggtgagcgcg
      481 gtcggcgaga ccctgccgca gcttgtcgcc ccgccggcgc gcgtcatctt ctgctgcttc
      541 tccgagcgct cggccgagtt gcaccgcgcc gcgctgagga agcgcggcga ataggcgagc
      601 gcctccgagg atcggaggta cgccgtgtgg aattaggtac gtccgagccg cctccgcgcg
      661 ccgcgctcgt ttcttgaagc ttctaccgca gctcgtatac cggcaccggc tcgctgattc
      721 cgcgcagcgt gtaagcctgc cgctcggtcg cggccatgcc ggcctcggct aaaatcttcg
      781 ccacattggc ggcttcgacg atcggtttgg tgacgaggat cgcgcttgga tcggcgaggc
      841 cctgcacgcg cgaggcgaca ttcaccgttt ggccgaaata atcctgccgc tcgtcgagca
      901 tcacggcgag gcagggcccc tcatgcaggc cgatattcag cgccagatcc tcgctgccgc
      961 gcgcttcgtt gatctggcgc atcgcgccgc gcatcttcat cgcggcgcgg accgcgcggt
     1021 cggggctggg gaaggtcgcc atcaccgcgt cgccgatcgt tttgaccacg gcgccgcctt
     1081 cagccgcgac cgtattcaac agcgcgctga aatgatcgcg cacaaggtcg aaggcggcga
     1141 ggtcgccgac ccggtcatag agcgccgtcg aaccgcgcaa gtcggtgaag aggatggtca
     1201 ggttggtgat cttgaagcgc tggtccttat ccagcgtgcc ggtccgatag agatcgcgga
     1261 aggtttggtt gctaagcagg cgagtcgcgg tgaggaaagg ccgccgcgcg ccgagaatgc
     1321 ggtgcatcgc ctcgccggcg agccacaagc cgggaatcgt ccgcttgctg gttctatttt
     1381 caaacgtgat ccgcgccggc ccgggctgca agacgagctt gccggcatgg gcgcgaaggt
     1441 cgctgaagac gagcgacacg ttgcggcgct catgcgtcgg ctcgcccgag acttccatga
     1501 acaaggtcgt gtgggtgacc ggatcgaagg cgatgatgaa cgattcgggc aaggtcagcg
     1561 acatcgccgc tttttcgccc ggcgcaagct ccatggcgtc gagcgtcagt tcgttcatcg
     1621 ccgcgtcgat atcgccgggc aggtcgacgc cggagcccca gaaaatctgg cgcgcatatt
     1681 cgccgagcgg cagcgtttcg ggatcgtgcg ccgcgatgtg gcggacgcgc ggattgacgg
     1741 tgaaggtcac ctccaccagt tcgtcgaggg tcggctcgta ggagccggcg caaagcgagc
     1801 agaaatattc gtgccggtcg agcgtcttga gtccgctccc cgtctccaga acgccgccgc
     1861 agcccgggca cagcacgttc caggaaaggt cgaacaagcc gaggcgggcg gcgtggatca
     1921 gggtgctgat cgtcgcctct tcatcatggc cgcgctcggc ggcataggcc aaggcgttga
     1981 tgcgattgag ggcgcgatcc tcgccggtcg cgacgaggct cttcagcgaa gcggcgacct
     2041 cggcgtcggc cgactgggcc aagagcgtcc aaagggtgtc gatctcagcc atggatgtcc
     2101 tctccgcgat gacccgcccc cgctcggcct cgcctttcgg agcgctaaaa taggtttgcg
     2161 ggcgctccct ttcaacaagg cgcgcgccgt cgcgaggcgc aatggatttc gctgctgaca
     2221 aaaactgacg cttggcggcg ttaggtcgac atctgcgcgg cccatcgcgt tcgaggagcg
     2281 accggatgaa gacgatcgaa gcgcaatgcc gctgcggcgc gatcggtttg aaaatcgccg
     2341 gcgaaccggt cgtgcagctc tattgccatt gcggcgattg ccaggccgct cacggcgcgg
     2401 cttatgcgcc ggcgacgatc tatcccgcgc cggcggtgga ggtcgtcagg ggcgagccgg
     2461 cgccgatggt cgtaaggaca acgcaaagga tgcgctgcgc ggcttgcggc acgtacctgt
     2521 tttccgagct tgccagcgct ggggtcagga gcgtcagcgc aatgcttctg cccaagggcg
     2581 aattcaagcc gcaatttcat gtccagtgcg aggacgccgt cctgccggtt gtcgataatt
     2641 tgcctcatta caagcgcttt ccggcggcgt ttggcggcgc cgagaccttt gtcgattggt
     2701 aagcgccaca acgcgccgga cgccattgcg aggagcgaaa cgacgaagca agccacgctc
     2761 tatgaatgga ttgcttcgcc tcgctcgcaa tgacgccgct gctactttcc cgccggcgcc
     2821 gccggactcg gcagcagcgt ctggttcggc ttcaggaaag gcttcagcgc ggcgtaaggt
     2881 acgacgagcg ggttgaaggg atagccgccc gagcagacgc cctccactgc gtgcgggaag
     2941 gcgagaaagc gaaaggcgag cccttgagcg gtcggataga ccgccttggc gatcgcagcg
     3001 ccgtcggagc ggagcgcgtc aagaaggact tgcccgcaat gcggatcgtc gtcggaattt
     3061 tgcggcgcgg ctttctcgcc ggccgcgatc ctggcgcgca gatcgctcat ccaaagcgcg
     3121 tcgaaagcgg cgcgccgctt ggccgcctgg atgtcgagcg cgcggccgag gcctgacggg
     3181 ccgagcgggt cggcgtcatc cccgccggcg atcagcgcgc catcggcaag atcgaacgta
     3241 tagaccgcga cttcgagatt gggatgcgcg ccgccgcaaa agaccatgcc tgatttctcg
     3301 atcgtcagca gccgggcggt ggcgtaggtc ggcgtcaagg ccgccgccga atccttggcg
     3361 cccttctcct cgttcttcag cgcctcggcg ccgttgggcg cggccgccgc gcgctgggct
     3421 tgcagacatt cgcgatcctc aaccctttgc gcgcgctgca aacggtcgag cccggcattg
     3481 accgcggcca ggtccgcccc ggccggcgcg cgggtgagac gcgggacgag gccgccgaac
     3541 agcggatcgc gcatcggtcg ataggagacg gtcccgagcg aagcctcggc ccccgccgcg
     3601 acgacgggcg gcgcgccgcg aagccagcgc tcgtcatagg cgcccggccg atcccctgtc
     3661 gtcagggcgc caaccgattc gtcgtcggtt gagattctgg tcagcgccag cggccgctca
     3721 tggccgccat gcgcatcctt ccagacgcct ttcagcgcgc cgtcggcgaa gcgcgcggtc
     3781 caggccccgc tgacaagttc aggatgttct ttcgccgcgt cctcgtcgac gcccggggcg
     3841 agcgtctcga ccatcgtcag cgcgccgccc gcgtcggaaa cgatcaggcc gagatcgttc
     3901 gcctggccga gatagacgta gcgcgcgccg ctcggcgcca aagccgcgac gacatgcgcc
     3961 ttgccgatga cgccctcata gacgccttgc caggtcggcg tgtcggaatc cgccgcgacg
     4021 gggccggcgg cgagggcgcc gagaagaacg atggcggccc ggcgcgatcg catcccgccg
     4081 aacgtgcggg acggccgaag aaaggcctca accacggccg agcagggccg agacggcggc
     4141 ttctgcgtcc ttgctgtccc aggcggcggg cccggccatc accccgactt cgcagccctc
     4201 cttgtcgatc agcagcgtcg tcggcaggcc gagcgccttg ccggcgactt tcagcgcctc
     4261 gaaggaatcg gcggtggggt cggcgtagag gctgagattc ttgacgccgt tgtcggcgaa
     4321 gaaggctttc ggcttgtcga gccgggtcgt gtcgatattg accgcgacga cctggaagtc
     4381 gggcccgccg aatttcgcct ccagccggtc gagggccggc atctcggaac ggcacggcgc
     4441 gcaccaggtc gcccagagat tgagcagcac ggtgcggccg cggaaatcgg ccaaggtcag
     4501 ctttttgccg tcggggtcct tgaacgacac gtcgatcgcc ggtttcggcg ttttgtcgac
     4561 gctgagcgcc gcgacctcgc ccttggcgag cggcgtcagg cgctgggcga tcgccgcggc
     4621 tttagggcag agcgcggcct cgtccttgcc cgaggcgccc ttcccgtata ggacggcgaa
     4681 ccccgccgcc gcgatggcga ttgcgaagac cgcgaccagc ggcgttttgc gccaaaaccc
     4741 ggcccgttca gctctcattt gttctttccg aggcgtctat gagcaacaag atgtggggcg
     4801 gccgcttcga agccggcccc tcgaaaatca tggaggagat caacgcatcg atcgacttcg
     4861 accgcaagct cgccgcccag gacattgcag gctccaaggc tcatatcgcc atgctggccg
     4921 aaaccggcat cgtcgccaaa gctgacgccg ccgccatcgc caaaggtcta gacaaggtcg
     4981 gcgccgaaat cgaggccggc cgtttcgctt tttcgcgcgc tctcgaggac atccatatga
     5041 acgtcgaggc gcggctcgcc gagctggtcg ggccggccgc cggccggctg cataccgccc
     5101 gttcgcgcaa cgaccaggtc gcgctcgatc tgaagctctg ggtgcgcgac gcgatcgaca
     5161 cgctcgacag gcaggtcgcc ggcttgcagc aggctctggc cgagcgggcg ctcgaacatg
     5221 ccggcgcggt catgccgggc ttcacccatt tgcaatcggc gcagccggtc acgttcggcc
     5281 atcacctgct cgcctatgtc gagatgctgg cgcgcgaccg cggccgtttc gccgacgccc
     5341 gcaagaggct caacgaatgt cctctcggcg ccgcggccct ggccggcacg tcctttccga
     5401 tcgatcgcgc catgaccgcc aaggcgctcg gcttcgaccg gccgaccgcc aattcgctcg
     5461 acagcgtcgc cgatcgcgat ttcgcgctcg aagcgctcgc cgcggcggcg atcgccgccg
     5521 cgcatctctc gcgcttcgcc gaggagatcg tactctggac gacgccgcaa ttcggcttcg
     5581 tcaggctgtc ggacgcgttc tcgaccggct cctcgatcat gccgcagaag cgcaaccccg
     5641 acgccgccga actcgcccgc ggcaagaccg ggcggatctt cggcgcgctg gtcgggctgc
     5701 tcaccgtgat gaagggcctg ccgctcgctt acgccaagga catgcaggag gacaaggaag
     5761 gcgtgttcga cgcgctgcag acgctgtcgc tggtcgtcgc ggcgatgacc ggcatggtga
     5821 gggacatgac gcccgacgcc aagcgcatga aggcggcggc cggggccggc tatgcgacgg
     5881 cgaccgacct cgccgattgg ctggtgaggc ggctc
//
LOCUS       DATGJW010000231         6303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_457356, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000231 DATGJW010000000
VERSION     DATGJW010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6303
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_457356"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1480)
                     /locus_tag="VKU03_07235"
     CDS             complement(<1..1480)
                     /locus_tag="VKU03_07235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019171641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ71093.1"
                     /translation="MALSGKSSTRRGSRPSERGALDLWDDGPPFRFRGLPDPAAFWLF
                     LKRNARFIAAIAATVAILGAIALWLAFNQYAATALVLVDPRDVKATTTPEVLANVGGD
                     AIAVESMVQVARSDGFLGALVDQQGLANDSEFNRGAATGEARRAAAIDKLKSKLSIGR
                     RGATYVIDVTMKSRDAQKAARIANAAAKMIVDNQAQLRLGSNQRAIDFLGGKLADLRK
                     KVHDEEEAAAALKAGLKITDAGQGVLLQARRVADLNQQLVLAKTHTEEMRARVEQLRK
                     VRGNGDLDLPTTPEMTVLSALRQDYARLSQRATEKEAVLGERHPDVIALRAQIVDSKR
                     QIAAEEARLVGAAKNDYAEARQREAALADALAKAHALYRRLDLPQPERIGRRFPHEVS
                     GGQLQRFMIAMGLIEGPELVVCDEPTSALDVTTQVEVLTALKSVIRDGNTAGLFVSHD
                     LAVVAQIADRILVLRRGQLVESGTTREILTIPQEPYTKELVAA"
     gene            1627..3168
                     /locus_tag="VKU03_07240"
     CDS             1627..3168
                     /locus_tag="VKU03_07240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71094.1"
                     /translation="MQGESLQAGKARRMADLFASATLAFVLSAAPIVLHLISPSLAVA
                     FAFASGFAVALLLERAAPMLILVSFMFQTMFVAMASSHATQFSDLDAMKVYDFVITIG
                     VWTAIAGRVLVGKAPASPFLWRMILASTAILVLAGFYFLAGLPIDSRGAIIYMRNIGL
                     PALLFQICLFVASRNPLAMRSTATLLLALLAICGYFELLDLQRWLDVTNGWHYWDLAS
                     LPNRDARQFERQARETGQVLTGTVDFLTGSLFNTDLLSGLDLQVVRLQGPNFHPISFG
                     YSLAVLIAFLAAQGRKLLPLVAAPLLLFVGAKGAVALLIFSLAFCFAARSALSRLAPL
                     GLAGALVLYATFVFWTGAQNGDFHVLGLIGGLKGFLADPIGHTLAEGGNLSTNFAAID
                     WTKYQHQGSTDIAVESAIGVLFYQMGIAAAGVLAIYLFFARVAWRLFRALRAPALALA
                     AGSIAVILVNGLFQEEALFAPLALGLVMALAGLSFGAADRLVRATALNAPPARRGAGA
                     VGAPA"
     gene            3165..4283
                     /locus_tag="VKU03_07245"
     CDS             3165..4283
                     /locus_tag="VKU03_07245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402606.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLJ71095.1"
                     /translation="MSALAPRVMLVQTQAENAGAQQVSRMIAADFAARGFSVHQVFFF
                     RRTASFDRDANVEYCALERPSKPLAFLRFLKRLYATTRRIRPDVVIAFQHYGNVIAAP
                     IARLAGAKRIVANQVSAGALIGPAALAADKLLGALGGYDRIVVNSRHTEEMYAEYPRR
                     YRERMIRINHGFEDKTALASKAEARAAFALPQDAKLMGCAARLHPLKQLDAAIAVLPR
                     IAGAHLALAGQGPDRPRLEETARALGVADRVHFLGELPPEKIGLLHAALDCFVFPSAA
                     ETFGLAAVEAAQSGIPVVANDIGVLREVLCVGGEACALFADARDQPAFAVAVEAALFD
                     QPQIARLRAAGRRLKQRFPLSAMMDAYCALVRELTAAR"
     gene            4280..5830
                     /locus_tag="VKU03_07250"
     CDS             4280..5830
                     /locus_tag="VKU03_07250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71096.1"
                     /translation="MKIEIIVDRDKLRLAHLALRERLARAFPNAGVILSGADGAAPLP
                     AAVAQLLRFERFAYRRGRANLCDLSAMRLDAGQGDADIVVDLSSMDAAARPGQVRLRP
                     LFAGASGEAALIGALIAGAAPHVDIENGATGEVAASGLPSLEAADGLLGGMEAVFSRL
                     SILIEQAIRAPRRALPRRRFEARNASSAEAARYAAKSLAWSAARVLYHATCYSPHWRV
                     GWRFNDGPGVMERGGLEGPRWRVLADRGHDCYADPFPIVWRGRTCVFFESLDHRVNKG
                     VIGVVEFGPQGPIGEATTIIEEPWHLSYPFLIEAEGELWMIPESSASGRVPLYRCLDF
                     PYRWERREPLLEGIEAADATIFAHQGRYYMISVVREGIGGYSDTLAIHHAPSLFGPWR
                     AHAMNPILIDSSAARPAGRVVARSGALWRPVQDCSKGYGRALRLARIDRLDPDCFEQT
                     FAGSIESGPLWPGGRLHTVNRAGRLETIDGVALTPRFAPLRGLVAKRYEPGEAAEPAP
                     LAEAPAAL"
BASE COUNT          888 a         2116 c         2083 g         1216 t
ORIGIN      
        1 aggccgcaac cagctccttg gtatagggct cttgcggtat cgtgagaatt tcgcgcgtcg
       61 tacccgattc gaccaactgg ccgcggcgca ggacgagaat gcggtcggcg atctgcgcca
      121 ccacggcgag gtcgtggctc acaaagaggc cagcggtgtt gccgtcgcgg atcacggatt
      181 tcagcgccgt cagcacctcg acttgcgtag tgacgtcgag cgcgcttgtc ggctcgtcgc
      241 agacgacgag ctcgggacct tcgattagcc ccatcgcgat catgaagcgc tgcagttggc
      301 cgccggagac ctcgtggggg aagcgcctgc caatcctctc gggctgcggc agatcgagac
      361 ggcgatagag cgcatgcgcc ttggcgagcg cgtcggcgag cgccgcctcg cgctggcgcg
      421 cttcggcgta gtcgttcttg gcggcgccga cgagccgcgc ctcctcggcg gcgatctgcc
      481 gcttggaatc aacgatttgg gcgcggagcg cgatgacgtc cgggtggcgc tcgccgagca
      541 ccgcctcttt ctcggtcgcc cgctggctga gccgcgcata atcctggcgc agcgcgctga
      601 gcaccgtcat ttccggcgtt gtcggcaggt cgaggtcccc attgccgcgt actttgcgca
      661 attgctcgac ccgcgcccgc atttcctcgg tatgggtctt ggcgagcacc aattgctgat
      721 tgagatcggc gacccgtcgc gcctgcagca gcacgccctg gccggcgtcg gtgatcttga
      781 gcccggcttt cagcgccgcc gccgcctctt cctcgtcatg cacctttttt ctgagatcgg
      841 cgagcttgcc gccgaggaaa tcgatcgcgc gctgattgga gccgaggcgc aattgcgcct
      901 ggttgtcgac gatcatcttg gcggcggcgt tggcgatgcg cgcggccttt tgcgcgtcgc
      961 ggctcttcat cgtcacgtcg atgacatagg tcgcgcctcg ccggccgatc gacagcttgc
     1021 ttttgagctt gtcgatcgcg gcggcgcgcc gcgcctcgcc ggtcgccgcg ccgcggttga
     1081 attcgctatc gttggcgagg ccctgctggt cgaccaaggc gccgagaaag ccgtccgacc
     1141 gggcgacttg caccatgctc tcgacggcga tcgcgtcgcc gccgacattg gcgagaacct
     1201 ccggcgtcgt cgtcgccttg acgtcgcgcg gatcgaccag gacgagagcg gtcgcggcgt
     1261 attggttgaa agcgagccat agagcgatcg cgccgaggat tgcgacggtc gcggcgattg
     1321 ccgcgatgaa cctggcgttg cgcttcagga acagccaaaa agccgcaggg tccggcagcc
     1381 cgcgaaagcg gaagggcggg ccgtcgtccc aaagatcgag cgcgccccgt tcgctcggcc
     1441 gggacccgcg acgcgttgac gatttgccgg acagcgccat ctgacagcgg ctcgcttaaa
     1501 cggcctcggc gaggtcctcc atcgcggcct gtcaagtaca agatacacca agcgttaagc
     1561 aatggttact aaacctcgaa ttgccggcgc ggcggcgccg gtttgcggcg cagcgaggac
     1621 gattggatgc agggcgaatc gctgcaagcg ggcaaggcgc ggcggatggc cgacctcttc
     1681 gcctcggcga cgctcgcctt cgtcctgagc gctgcgccga tcgtcctgca tctgatcagc
     1741 ccgtctttag ccgtcgcttt cgccttcgct tcgggcttcg cggtggcgct cctcctcgag
     1801 cgcgccgcgc cgatgctgat cctcgtctca ttcatgttcc aaaccatgtt cgtcgccatg
     1861 gcctcgtcgc atgcgaccca attctccgat ctcgacgcga tgaaggtcta tgacttcgtc
     1921 ataacgatcg gcgtttggac cgcgatcgcc ggccgggtcc tggtcggcaa ggcgccggca
     1981 tcgccgttct tgtggcggat gattctcgct tcgacggcga ttctggttct ggcgggattc
     2041 tatttcctcg ccggcctgcc gatcgacagc cgcggcgcca tcatctacat gcgcaatatc
     2101 ggcctgccgg cgctgctgtt ccaaatctgc ctgttcgtcg cctcgcgcaa tccgctcgcc
     2161 atgcgctcga ccgcgacgct gcttttggcg ctgctggcga tctgcggcta tttcgagttg
     2221 ctcgatttgc agcgctggct cgacgtcacc aatggctggc actattggga tctggcgtcg
     2281 ctgcccaatc gcgacgcgcg gcaattcgag cggcaggcgc gcgagaccgg ccaggtcctg
     2341 accggcacgg tcgatttcct caccggcagc ctgttcaaca ccgacctgtt gagcgggctc
     2401 gatctgcaag tcgttcgctt gcagggtccg aacttccacc cgatcagctt tggctactcc
     2461 ctggctgtgc tcatcgcgtt cctggccgcc cagggtcgca aactgctgcc tctcgtcgcg
     2521 gcgccattgt tgctcttcgt tggcgccaag ggagccgtcg cgttgctgat cttttcattg
     2581 gccttctgtt ttgcggcgcg ttccgcattg agccgcctcg ccccgctcgg cctggccggc
     2641 gccctggttc tttacgcgac gttcgttttt tggacaggcg ctcaaaacgg ggactttcac
     2701 gtcctcggcc tgatcggcgg ccttaaaggt ttcctggccg atccgatcgg ccatacgctg
     2761 gccgagggcg ggaatctctc gaccaatttc gcggcgatcg actggacgaa ataccagcat
     2821 cagggatcga ccgacatcgc cgtcgaaagc gcgatcggcg tgctcttcta ccagatgggg
     2881 atcgccgcgg ctggcgtgct ggcgatttac cttttcttcg cgcgcgtcgc ctggcgcctg
     2941 ttccgcgccc tccgcgcgcc ggctttggct ctggccgccg gctcgatcgc cgtcatcctg
     3001 gtcaacggcc tcttccagga agaggcgctg tttgcgcctc tcgccttggg cctggtcatg
     3061 gcgctggccg gcctttcctt cggcgcggcg gatcgcctgg ttcgcgccac ggcgctgaac
     3121 gcgccgccgg cgcggcgcgg cgcgggggcg gtcggagcgc ctgcatgagc gcgcttgcgc
     3181 cccgggtgat gctggtccag acgcaggcgg agaatgccgg cgctcagcag gtttcgcgga
     3241 tgatcgccgc ggatttcgcg gcgcgcggct tcagcgtcca tcaggtcttc ttctttcgcc
     3301 gcaccgcttc gttcgaccgc gacgccaatg tcgaatattg cgccctggag cggccttcca
     3361 agccgcttgc tttcctgcgc ttcctgaagc gcctctatgc gacgacgcgg cggatccggc
     3421 cggacgtcgt catcgccttc cagcattacg gcaacgtcat cgccgcgccg atcgcccgcc
     3481 tcgccggcgc caagcgcatc gtcgccaacc aggtctcggc cggggcgctg atcggccccg
     3541 ccgcgctcgc cgccgacaag cttctcggcg cgcttggcgg ctacgatcgg atcgtcgtca
     3601 attcgcgaca cacggaagag atgtacgccg aatatccgcg ccgctaccgc gagcggatga
     3661 tccgcatcaa tcacggtttc gaggataaga cggcgctcgc ctcgaaagcc gaagcgcggg
     3721 ccgccttcgc gctgccgcag gacgccaagc tcatgggctg cgcggcgcgg ctgcatccgc
     3781 tcaagcaatt ggacgcggcg attgcggttc tgccgcgcat cgccggagcg catctcgctt
     3841 tggcgggcca ggggccggac cggcctcgcc tggaggagac cgcgcgcgcc ctcggcgtcg
     3901 ccgatcgggt ccatttcctc ggcgaattgc cgccggagaa gatcggcctt ctgcacgcgg
     3961 ctttggattg cttcgtcttc ccgagcgccg ccgaaacgtt cggcctggcg gcggtcgagg
     4021 cggcgcaatc ggggattccg gtcgtcgcca atgacatcgg cgtgctgcgc gaggttcttt
     4081 gcgtcggcgg cgaagcttgc gcgctgttcg ccgacgcgcg cgatcagccc gctttcgccg
     4141 ttgcggtcga ggcggccttg ttcgatcagc cgcagatcgc gcggctgagg gccgccgggc
     4201 gccggctgaa gcagcgcttt cccttgagcg cgatgatgga cgcctattgc gcgctggtcc
     4261 gcgaactgac ggctgctcga tgaagattga aatcatcgtc gaccgcgaca agctgcggct
     4321 cgcccatctc gctctcaggg agcggctcgc ccgcgctttt ccgaatgccg gcgtgatcct
     4381 gagcggcgcg gacggcgccg cgcccttgcc ggcggccgtc gcgcagctcc tgcgcttcga
     4441 gcgcttcgcc taccggcgcg gccgcgccaa tctttgcgat ctctccgcga tgcggctcga
     4501 cgccggtcaa ggcgacgccg acatcgtcgt cgacttgtcc tcgatggacg cggcggcgcg
     4561 ccccggccag gtgaggctgc ggccgctttt cgctggcgcg tccggcgaag ccgctttgat
     4621 cggcgcgctg attgccggcg ccgcgccgca tgtcgacatc gaaaacggcg cgaccggcga
     4681 ggtcgccgcc tcgggcctgc cctctctcga agccgctgac ggcctgctcg gcggcatgga
     4741 ggcggtcttc tcccgcttgt cgattttgat cgaacaggcg atcagggcgc cgcgccgggc
     4801 gctcccgcgc cgccgcttcg aggcgaggaa cgcgtccagc gcggaagcgg cgcgctacgc
     4861 cgccaaaagc ctcgcttgga gcgccgcgcg ggttctctat cacgcgacct gctattcgcc
     4921 gcattggcgc gtcggctggc gcttcaatga tggaccaggc gtgatggagc gcggcggcct
     4981 cgaagggccg cgctggcgcg ttctcgccga tcgcggccat gattgctacg ccgatccgtt
     5041 tccgatcgtc tggcgcgggc ggacttgcgt gtttttcgag agcctcgacc atcgcgtcaa
     5101 caaaggcgtg atcggcgtcg tcgaattcgg gccgcaaggg ccgatcggcg aagcgacgac
     5161 aatcatcgaa gagccttggc atctctcgta ccccttcctc atcgaggccg agggcgagct
     5221 ttggatgatc ccggaaagct ccgcgtccgg gcgcgtgccg ctctatcgct gcctcgattt
     5281 tccctatcga tgggagcgcc gcgagcctct gctggaaggg atcgaagcgg ccgatgcgac
     5341 aattttcgcg catcagggcc gttactacat gatttcggtc gtgcgcgaag ggatcggcgg
     5401 ctattcggac acgctcgcca tccatcacgc gcccagcctt ttcggcccgt ggcgggcgca
     5461 tgcaatgaat ccgatcctga tcgattccag cgccgcgcgg ccggccggca gggtcgtcgc
     5521 gcggtccggc gcgctgtggc ggcccgtgca ggattgcagc aaaggctatg gccgggcgct
     5581 tcgcctggcg cggatcgacc ggcttgatcc ggattgtttc gaacagacat tcgccggttc
     5641 tatcgaatcc gggccgctct ggccgggcgg cagattgcac accgtcaacc gcgccgggcg
     5701 attggagacg atcgacggcg tcgcgctcac gccgagattc gcgccgctgc gcgggctggt
     5761 cgccaagcgc tacgagccgg gcgaggccgc cgagccggcg ccgctcgccg aagcgccggc
     5821 cgcgctttag catgcggcgc gtgcaggtac gaattgacga agaaaagcgt gcatctttat
     5881 gaggttaagc tcgcgccgct tgtcggcgac aaatgcgcga caatgaagtt ttgtactaaa
     5941 agcgcaaacg ccgtcgcacg gaggggtcaa ggatcgcgag ccatagcgcc gagccggaag
     6001 agtcttctcc gagctcgcct cggctcgcgc cgcgaagcgc ttgtccttgg cgccgggcac
     6061 ggcgatggcg tgcaggatag ggcgcgtttt gcgctctcgc cactctgatt ggccagaaaa
     6121 cgcgctaagc aaaaccgcgt ttgggtacgg cgtcccgacg tttactgctt cgccctatcc
     6181 ggatcgcggc gcgagaggcg ccagccttcg ccgtgcagcc agcacgtttc gtcttcggcg
     6241 tggctgtcgg tgcggatcga tttgacctcg tggccgcgac tcttggccat tgcctgtctg
     6301 agg
//
LOCUS       DATGJW010000232         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1493793, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000232 DATGJW010000000
VERSION     DATGJW010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1493793"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..538)
                     /locus_tag="VKU03_07255"
     CDS             complement(<1..538)
                     /locus_tag="VKU03_07255"
                     /inference="COORDINATES: protein motif:HMM:NF012659.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T"
                     /protein_id="HLJ71097.1"
                     /translation="MPRLALSFALTLALASAGAPFAALAETAPAVATTQFDYYVMTLS
                     WAPGFCDIGGQETSSPECAAGSGDGFVVHGLWPNNEYRPNPEACLGGDATPADLADEH
                     GVYPNDRLAAYEYRKHGTCTGLSAHDYFATVREVKARLNIPPQFRAPRQPVRLAPEEV
                     ERAFIEANPNLRADNMAVT"
     gene            complement(558..1082)
                     /locus_tag="VKU03_07260"
     CDS             complement(558..1082)
                     /locus_tag="VKU03_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71098.1"
                     /translation="MNRLIWGFLILLSLCSLARAGGGGPAQLNLYDTPAEARWAPFFN
                     NMPACDDASVLSTIMSRFGATQREFWDPNLAIEGLDRVREIGFRSNGLSYIPRRYCVA
                     RAAMSDRRTRTVVYAVATQTGIIGNTWGVEWCVVGVDPTLAYAPDCEVIRPILEREIG
                     KTKWLAEYGLKAGY"
     gene            complement(1347..1832)
                     /locus_tag="VKU03_07265"
     CDS             complement(1347..1832)
                     /locus_tag="VKU03_07265"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176673.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HLJ71099.1"
                     /translation="MEIDAEIFVLLGFLCFLGLLVYLGAHRTILKALDARGEAIAAEL
                     AQAAKLRDEATALLASFEKKTAEAEANAAAIVAEARGQAAQLADEAAERLKEFIARRT
                     RQAEAKIALAEAQAAAEVRAAAAEHAAKAAEIVLADQMQGAAGADLVAREIGEIKRRL
                     S"
     gene            complement(1832..>2359)
                     /locus_tag="VKU03_07270"
     CDS             complement(1832..>2359)
                     /locus_tag="VKU03_07270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008839272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HLJ71100.1"
                     /translation="VGHDGGAHPSFPPFDSSTFPSQLVWFAIAFGFLYWYMSKRGLPQ
                     LGAVIEGRKARIARDLDDATAMQQKADAAAAAHERTLAEARAKAQSLAQAARDAAAAD
                     ARAKRQALEDELAVKLAAAEREIAAMRARAMANVAEVAREAAGAIVERLGGRPADPAS
                     VAAAVRSIPSPSGAA"
BASE COUNT          353 a          810 c          811 g          385 t
ORIGIN      
        1 aggtgaccgc catattgtcg gcgcgcagat tggggttcgc ctcgataaaa gcccgctcga
       61 cctcctccgg cgccagtcgc accggctggc gcggcgccct gaattgcggc gggatgttga
      121 gccgcgcctt cacctcgcgg acggtggcga aatagtcatg cgcgctcaag ccggtgcagg
      181 ttccatgctt gcggtattcg taggccgcga ggcgatcgtt gggatagacg ccatgctcgt
      241 cggcgagatc ggccggggtc gcgtccccgc ctaagcacgc ttccggattg ggccgatatt
      301 cattgttcgg ccacagcccg tggacgacga agccgtcgcc cgagccggct gcgcattcag
      361 gcgacgacgt ctcctggccg ccgatgtcgc agaaaccggg cgcccacgac agggtcatca
      421 cataataatc gaactgcgtg gtcgcgacgg ccggcgcggt ctcggcgagg gccgcaaaag
      481 gcgcgccggc cgaggccagc gccagggtca aggcgaagct caacgccagg cgcggcatcc
      541 ggcccgcctt ccgccggtca atagcccgct ttgaggccgt attcggcgag ccacttcgtc
      601 ttgccgatct cccgctcgag gatcgggcgg atgacctcgc agtccggcgc gtaggcgagg
      661 gtcgggtcga cgccgaccac gcaccactcg acgccccagg tattgccgat gatgccggtc
      721 tgcgtcgcca ccgcgtagac gacggtccgg gtcctccggt cggacatcgc ggcgcgggcg
      781 acgcaatagc ggcgcggaat gtacgagagg ccgttggacc ggaagccgat ttcgcgcacg
      841 cgatcgaggc cctcgatcgc cagattcgga tcccagaatt cgcgctgggt cgctccgaac
      901 cgactcatga tcgtcgacag cacgctcgca tcgtcgcagg cgggcatatt gttgaaaaat
      961 ggcgcccagc gcgcctcggc cggcgtgtcg tagaggttga gctgggccgg gccgccgccc
     1021 ccggcgcggg cgagcgagca cagcgaaaga aggatcaaaa aaccccagat caagcggttc
     1081 atgctttccc ctcttccggg cgtctgcggc ccgcccccgg caccgcagct tgcaagcctc
     1141 gtccagccga caataggctc ctcgttggtc aaatcaagga ttcctggcgc cgcgcgccat
     1201 cttccctggg cgtgatgaga tcggtttgaa actgggctgt catcgcgagg agcggagcga
     1261 cgtggcgatc cagaggcggt agggcgctac gactcctcga ttgcttcgct tcgctcgcaa
     1321 taacgttctt gcggcgccct acccgctcag gagagccggc gcttgatctc gccgatctcg
     1381 cgcgccacca gatcggcgcc ggcggccccc tgcatctggt cggcgagcac gatttccgcc
     1441 gccttggccg cgtgctcggc cgccgccgcg cggacctccg ccgcggcctg ggcttcggcg
     1501 agcgcgatct tggcctcggc ctgcctggtg cgccgggcga tgaattcctt cagccgctcg
     1561 gcggcctcgt cggccaactg ggcggcctgg ccccgcgctt cggcgacgat ggcggcggcg
     1621 ttggcttccg cttcggcggt ctttttctcg aaactcgcga gcagcgccgt cgcctcgtcg
     1681 cgcaatttcg ccgcctgagc gagctcggcg gcgatcgctt cgccgcgcgc gtcgagcgct
     1741 ttcaggatcg tgcgatgggc gccgagatag acgagcaggc ccagaaagca caggaagccg
     1801 aggaggacga agatctccgc gtcgatttcc atcaggcggc tccgctcggc gaggggatag
     1861 accggacggc ggcggcgacc gaggcgggat cggccgggcg tccgccaagg cgctcgacga
     1921 tcgcgcccgc ggcctctcgg gcgacctcgg ccacattggc catcgcgcgg gcgcgcatcg
     1981 cggcgatctc gcgctcggcg gccgcgagct tgaccgcaag ctcgtcctcg agcgcctgcc
     2041 gcttggcgcg cgcatccgcc gccgcggcgt cgcgcgcggc ctgggcgagg ctctgcgcct
     2101 tcgcgcgcgc ttcggccagt gtcctttcat gggcggcggc ggccgcatcg gctttttgct
     2161 gcattgccgt cgcgtcgtcg aggtcgcggg cgattcgcgc cttgcggccc tcgatcaccg
     2221 cgccgagttg cggcagtccg cgcttggaca tgtaccagta caggaatccg aaggcgatcg
     2281 cgaaccacac gagctgggaa ggaaacgtgc tcgaatcgaa tggaggaaag ctcggatgcg
     2341 cgccgccgtc gtgaccgac
//
LOCUS       DATGJW010000233         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_251421, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000233 DATGJW010000000
VERSION     DATGJW010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251421"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..456)
                     /locus_tag="VKU03_07275"
     CDS             complement(<1..456)
                     /locus_tag="VKU03_07275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320071.1"
                     /note="responsible for the amidation of carboxylic groups
                     at position A and C of cobyrinic acid or hydrogenobrynic
                     acid; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobyrinic acid a,c-diamide synthase"
                     /protein_id="HLJ71101.1"
                     /translation="MSAPGLVIAAPRSSSGKTTLALGLMRALARRGLKVAGFKCGPDY
                     IDPAFHEAATGRRGVNLDSWAMSPALLSGLATESSAAADFVLCEGLMGLFDGVPAQPG
                     RSGSTADIAAAFGWPVLLVLDVSGQSQSAGAAALGCKAFDPRIEIGGIIL"
     gene            complement(453..1235)
                     /gene="cobA"
                     /locus_tag="VKU03_07280"
     CDS             complement(453..1235)
                     /gene="cobA"
                     /locus_tag="VKU03_07280"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012606335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HLJ71102.1"
                     /translation="MSEAHPADIPFEPGTVWLVGAGPGDPGLLTLHALDALKKADVIV
                     YDALVSEGVLALAGPEARREFAGKRGGRPSHSQKDITLRLIELARQNLRVLRLKGGDP
                     FVFGRGGEEVKALAEAGVRFRVVPGITAGIGGLAYAGIPATSRETNQALILVAGHPAA
                     DGEDRIDWEALAACGQPLVVYMGLSRLAAIAARLIAGGMAPETRLALLFEATLPGQIV
                     IETTLAGARDHPPQGPGAAPALVVIGAIVGLRDALLAHLLPS"
     gene            1287..>2218
                     /locus_tag="VKU03_07285"
     CDS             1287..>2218
                     /locus_tag="VKU03_07285"
                     /EC_number="2.1.1.195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-5B (C(1))-methyltransferase"
                     /protein_id="HLJ71103.1"
                     /translation="MSEVKALRYGWTTGCCAAAAASAAFEALIAGEFPDPVGVLLPNG
                     ARPSFALALSERGADYARAGVVKDAGDDPDVTHGVTVIATVRRGPAGGGIAFRAGPGV
                     GTVTKPGLPIAPGEPAINPAPRRMIADALTRIAERQGVAADAIVEISIPGGEKIAEKT
                     LNPRLGIKGGLSVLGTTGIVVPFSCSAWIHSIHRGVDVARACGLTHLAGATGSTSEEA
                     VKRLHGLAEEALIDMGDFVGGFLKYLRKHPVETVTIAGGFAKMTKLGQGLLDLHSRRG
                     EVDLDWLAALAASAGLGEELAARIRAANTAMEAHA"
BASE COUNT          333 a          776 c          784 g          325 t
ORIGIN      
        1 gaggataatt ccgccgatct cgatgcgcgg gtcgaacgcc ttgcagccca aagccgccgc
       61 gccggccgat tgcgactggc cgctgacgtc gaggacgaga agcacgggcc agccgaacgc
      121 cgccgcgata tcggcggttg agccgcttcg ccccggctgc gccggaaccc cgtcgaacag
      181 gcccatcagg ccttcgcaga gaacgaaatc ggcggcggcg ctcgactccg tcgcgaggcc
      241 gctaagcagc gccgggctca tcgcccacga atcgagattg acgccgcgcc gtccggtcgc
      301 cgcttcgtgg aatgctggat cgatataatc cggcccgcat ttgaagccgg cgactttgag
      361 gccgcggcgg gcgagggcgc gcatcagtcc aagcgcgagc gtggtcttgc cgctgctcga
      421 gcgcggcgcg gcgatgacga ggccgggcgc gctcatgacg ggaggagatg cgccagcagg
      481 gcgtcgcgca agccgacgat cgcgccgatc acgaccaaag ccggcgcggc gcctggcccc
      541 tgcggcggat ggtcgcgcgc gccggccagc gtcgtctcga tcacgatctg gccgggcaac
      601 gtcgcttcga acagcaacgc cagccgggtt tccggcgcca tgccgccggc gatcaggcgc
      661 gccgcgatcg cggcgaggcg cgacagcccc atatagacga cgagcggctg tccgcaggcc
      721 gcgagcgcct cccagtcgat ccggtcctcg ccgtcggcgg cgggatggcc ggcgacgagg
      781 atcagcgcct ggttggtctc gcgcgacgtc gccggaatgc cggcgtaagc aaggccgccg
      841 atcccggcgg tgattcccgg cacgacgcga aagcgaacgc cagcctcggc cagcgccttg
      901 acctcctcgc cgccgcggcc gaacacgaac ggatcgccgc ctttcaggcg caacacgcgc
      961 aagttctgcc gcgccagttc gatcagccgc agcgtgatgt ctttctggct gtgcgacggc
     1021 cggccgccgc gcttgccggc aaattcgcgc cgcgcctccg gcccggcgag cgccagcacg
     1081 ccttccgaga ccagcgcgtc atagacgatc acgtcggctt ttttcagcgc gtcgagggcg
     1141 tgcagcgtca gcaggccggg atcgcccggc ccggcgccga ccagccacac ggttcccggc
     1201 tcgaacggaa tgtcggccgg atgcgcctcg ctcatgccac gcctttcggt ttcgagcttt
     1261 caggcaaggc gtctattgtc gcggtcatga gcgaggtcaa ggcgctgcgc tacggctgga
     1321 cgacgggatg ctgcgccgcc gcggcggcga gcgcggcgtt cgaggcgctg atcgccggcg
     1381 aatttcccga tccggtcggc gtgcttttgc cgaacggcgc gcggccgagc ttcgctttgg
     1441 cgctcagcga gcgcggcgcg gattacgcgc gggccggggt ggtcaaggac gccggcgacg
     1501 atcccgacgt gactcatggc gtcactgtga tcgccacggt gcgacgcggc ccggcgggcg
     1561 gcgggatcgc gttccgcgcc gggcccggcg tcggcacggt cacgaagccg ggcctgccga
     1621 ttgcgcccgg cgagccggcg atcaatccgg cgccgcgccg gatgatcgcc gatgctctga
     1681 cccggatcgc cgagcgccaa ggcgtcgccg cggacgcgat cgttgaaatc tcgattcccg
     1741 gcggcgagaa gatcgccgaa aagacgctca atccgcggct cgggatcaaa ggcggcctct
     1801 ccgttctcgg cacgaccggc atcgtcgtgc cgttttcctg ctcggcctgg attcattcga
     1861 tccatcgcgg cgtcgacgtc gcgcgcgctt gcggcctgac ccatctcgcc ggcgcgaccg
     1921 gctcgacctc cgaggaggcg gtcaagcgcc tgcacggatt ggccgaggag gcgctgatcg
     1981 acatgggcga tttcgtcggc ggcttcctca aatatctgcg caagcatccg gtcgagacgg
     2041 tgacgatcgc cggcggcttc gccaagatga ccaagctcgg ccaaggcctg ctcgatctgc
     2101 attcgcggcg cggcgaggtc gatctcgatt ggctggcggc gctcgccgca agcgcggggc
     2161 ttggcgaaga gttggcggcg cgcattcgcg ccgccaatac cgcgatggag gcccatgc
//
LOCUS       DATGJW010000234         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1494347, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000234 DATGJW010000000
VERSION     DATGJW010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1494347"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..662
                     /locus_tag="VKU03_07290"
     CDS             <1..662
                     /locus_tag="VKU03_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="patatin-like phospholipase family protein"
                     /protein_id="HLJ71104.1"
                     /translation="NPLNINPLRDHLVKAIDFEKIRASNDIKLMIAATNVFTGKGVIF
                     RRHELTADHIMASACLPRLFQAVEINGVPHWDGGYSGNPPLWPLFYETHTDDAIIVQI
                     NPIERRETPRTARDILNRQDEITFNSHLLAEMRAVNFVTRLIDRGKLQRGDYRRALLH
                     RIGGDGKLESFGPGTKLDTSWPFLLRLRDLGRDAAKQWLKDHFDDIGQRGTLDLPAII
                     S"
     gene            complement(889..1425)
                     /locus_tag="VKU03_07295"
     CDS             complement(889..1425)
                     /locus_tag="VKU03_07295"
                     /inference="COORDINATES: protein motif:HMM:NF019430.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HLJ71105.1"
                     /translation="MKNLVFVTLAVASLAGSAVSINALAANDPPAGAELRMEHSAALL
                     DAHLAGLKAGLKLTADQEKNWPAFENAVRQIAKERMDQFQAIRAERERDGARPSPIDH
                     MRMMSERLAKGSAELKTLADAASPLYASLDDNQKRNFGPLFRDLVREGREDARHWRHA
                     MWREHERDGDHDRPASAQ"
     gene            1667..2446
                     /locus_tag="VKU03_07300"
     CDS             1667..2446
                     /locus_tag="VKU03_07300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldolase/citrate lyase family protein"
                     /protein_id="HLJ71106.1"
                     /translation="MVEGGIVATLARRIKAGEPVFAAWVGINEPMIAEQLARDGFDAV
                     VMEMQHGAVDVAGALRGVAAIALAGKPAIVRIPIGDFSTASRVIDAGAAAVIAPMINS
                     GAEARQFADFMKFPPLGQRSWGPRGVLPLTGYDTGDYLRKANAFTLAIAMVETRAALA
                     ALDEILATPGIDGVFVGPSDLSIALSNGDIVDAQSAAVDAALTKIAAAAKAHGKFAAA
                     FCHTGERAKDLVSRGFSLCSVGTDQLLLRLAARQELAKARG"
     gene            2801..2956
                     /locus_tag="VKU03_07305"
     CDS             2801..2956
                     /locus_tag="VKU03_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492360.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HLJ71107.1"
                     /translation="MKLAFIARYLEERRRYWKVLHELSAYTDRELQDIGIDRIDIVDI
                     ARQAARG"
BASE COUNT          527 a          951 c          935 g          557 t
ORIGIN      
        1 tcaatccgct caacatcaat ccgctgcgcg atcacctcgt caaggcgatc gatttcgaga
       61 aaatccgggc gagcaacgac atcaagctga tgatcgccgc caccaacgtc ttcaccggca
      121 aaggggtgat cttccgccgc cacgagctga cggccgacca tatcatggcc tcggcttgcc
      181 tgccgcgcct cttccaggcg gtcgagatca acggcgtgcc gcattgggac ggcggctata
      241 gcggcaatcc gccgctctgg ccgctctttt acgagaccca tacggacgac gcgatcatcg
      301 tccagatcaa tccgatcgaa cgccgcgaga cgccgcgcac ggcgcgcgac attctcaacc
      361 gccaggacga gatcacgttc aacagccatc ttctcgccga gatgcgcgcg gtgaatttcg
      421 tcacccggct gatcgaccgg ggcaagctgc aacggggcga ttaccggcgg gcgctgctcc
      481 accgcatcgg cggcgacggc aagctcgaaa gcttcggtcc cggcaccaag ctcgacacgt
      541 cctggccctt cctgctgcgc ctgcgcgatc tcggccgcga tgcggcgaag caatggctga
      601 aggatcattt cgacgatatc ggccagcgcg gcacgctcga cctgccggcg atcatttcgt
      661 gagcgggcag ccggaaagct gagggcgtac cctcatccgg ccctttcgag ccaccttctc
      721 ccgcaagcgg gagaagggat cgtggccgcg gtcggcgacc gcggctacag ccgctgctgt
      781 cgagaaacgt cggcgcgccc cctctcccgc ttgcgggaga gggttggggt gagggtaagc
      841 cgcggcggtt ggttataacg gcctctggca cgcgccgccc gtggcctgct attgcgcgct
      901 tgcggggcgg tcgtgatcgc cgtcgcgctc gtgctcgcgc cacatcgcgt ggcgccaatg
      961 cctcgcgtct tcgcggccct cgcggacgag gtcgcggaag agcggtccga aattgcgctt
     1021 ctggttgtcg tcgaggctgg cgtaaagcgg actcgccgca tcggcgaggg tcttaagctc
     1081 ggccgagcct ttggcgaggc gctccgacat catccgcata tggtcgatcg gcgacggccg
     1141 cgcgccgtcc ctttctctct cggcgcgtat cgcctggaac tggtccatgc gctccttggc
     1201 gatctgccgc acggcgttct cgaaagccgg ccagttcttt tcctggtcgg cggtcagttt
     1261 caggccagcc ttgaggccgg cgagatgcgc gtcgagcagc gcggcgctat gctccatgcg
     1321 aagctccgcg ccggccggcg gatcgttcgc cgcgagcgcg ttgatggaga cagccgaacc
     1381 ggcaagactc gcgacggcga gggtgacaaa gacgagattt ttcatcgttc gcctcttggg
     1441 agggacattt tcgaccctat caggagtcgt ttcggcccgg attaaagtca aattacatca
     1501 gcgtaatgtc gccggtcgtc gagcgaaggt acgagagcct tccgacttta aggattcgac
     1561 gacttggcgc ttcgttccgc ggcccacgtc gcagcgctgg gcgccggtct tcgaaggatg
     1621 ctaagaaacg tcgctttctc aacaaacggt cggaacgagg ttggaaatgg tcgagggcgg
     1681 aattgtcgcg acgctggcgc ggcggatcaa agcgggcgag ccggtcttcg cggcttgggt
     1741 cggcatcaac gagccgatga tcgccgagca gctcgcccgc gacggcttcg atgcggtcgt
     1801 gatggagatg cagcatggcg cggtcgatgt cgccggcgcc ttgcgcggcg tcgccgcaat
     1861 cgccctcgcc ggaaagccgg cgatcgtccg catcccgatc ggcgactttt cgaccgcctc
     1921 gcgcgtgatc gacgcggggg ccgcggcggt gatcgcgccg atgatcaata gcggcgccga
     1981 ggcgcggcaa ttcgccgatt tcatgaaatt tccgccgctc ggccagcgca gctggggccc
     2041 gcgcggcgtg ctgccgctga ccggctatga caccggcgat tacttgcgca aggccaacgc
     2101 cttcaccctg gcgatcgcca tggtggagac gcgcgcggcg ctcgccgcgc tcgacgagat
     2161 tttggcgacg ccgggcattg acggcgtatt tgtcggcccc tccgacctct ccatcgcctt
     2221 gagcaatggc gacattgtcg atgcgcaaag cgcggcggtc gacgccgcct taacgaagat
     2281 cgccgccgcc gccaaggcgc atggaaaatt cgccgccgcc ttttgccata ccggcgaacg
     2341 agccaaggat ttggtcagcc gcggcttttc gctgtgctcg gtcggcaccg atcaattgct
     2401 gctgcgcctc gccgcccgcc aggaattggc caaggcgcga ggctaagcaa tcagcaatgc
     2461 ggggccgtcg gccccggcaa tggttaacct aatgttgcgt cgcggcaaaa ttgtccgcga
     2521 catctcgccg ctggctatgc acaaagagca tgcaattgat cagatcgtcg ccagtttttt
     2581 ggctttatgt ccgctgaaac ctgtattttc ccgatcagtc acggttctga catctcatgt
     2641 tcatgcaaat ttcacatgcc ctgacacgat gcgccttgac ttgtgcattg caacaacata
     2701 tgtagcggcc ggtctcggag aagaaacgtc cagggtgtaa tgagctcgcc gatcggcttt
     2761 ccggcggatc ggcgagaaac agcgagaagg agttgcaaaa atgaagctcg ccttcattgc
     2821 ccgctacctg gaagagcgcc gccgctactg gaaagttctg cacgaattgt cggcctatac
     2881 cgaccgagaa cttcaggata tcggcatcga tcgcatcgac attgtcgaca tcgcccgtca
     2941 agccgcgcgc ggctgagccg gcctccactt
//
LOCUS       DATGJW010000235         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1181841, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000235 DATGJW010000000
VERSION     DATGJW010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1181841"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..664
                     /locus_tag="VKU03_07310"
     CDS             <1..664
                     /locus_tag="VKU03_07310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008596435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HLJ71108.1"
                     /translation="QPRVFLFDEPLSNLDAKLRVDMRVEIKRLHHQTGTTIVYVTHDQ
                     IEAMTLATRIAVLKDGELQQVGSPYEVYNSPANLFVADFMGSPSMNLIKAKVARNNGA
                     ASIVLERAGAEPIVMPAPTSADATKLRDGGAVIFGIRPEAVSDPESADRNSKHVATFP
                     CRVDLVEPAGSDTFVVTSIAGKEVTARMRADADVRPGQNVSFAFNLDKAVLFDPDSTQ
                     RI"
     gene            904..>2200
                     /locus_tag="VKU03_07315"
     CDS             904..>2200
                     /locus_tag="VKU03_07315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018236039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLJ71109.1"
                     /translation="MNAQRDVDVAVIGSGMGGASFAAGLAPSGARIVIIERGERIAAS
                     ALARDPRAIFQHGHFRPQEFWYDGAGRPFNPGNYYYVGGNTKLYGAVLLRYRAEDFRP
                     IEYAEGATPGWPFPYEELEPWYSRAEALYQARGALGEDPTEPPHSKPYPFPPVPDEPA
                     IAALRQRFKQIGLHPFSLPLGVDIEAWLKGGQTPWDAFPNTDSGKMDAETCGLAAALK
                     HENVSLETGARVTRLETDPAGKRIESVVYQKNGETLKLSPKAVVLAAGAVNSALLLLA
                     SANGAMPSGLANRSDQVGRHFMNHNLSFALALHPLRKNDSVYQKTIGLNDFYLSDGRG
                     GPPLGNVQMVGKISGPILKSELRRAPETALGVLARRTFDFLFMSEDLPSPESRVRLDG
                     SRVVLDWRRSNIAAHNGLVVRMREMLKEAGFPIVLVKAVD"
BASE COUNT          401 a          734 c          703 g          362 t
ORIGIN      
        1 ccagccgcgc gtcttcctgt tcgacgagcc gctctccaat cttgacgcca agctgcgcgt
       61 cgacatgcgg gtcgagatca agcggctgca tcatcagacc gggacgacga tcgtctatgt
      121 cacgcatgac cagatcgagg cgatgaccct cgccacccgc atcgcggtcc tgaaagacgg
      181 ggaattgcag caggtcggct cgccttacga ggtctacaat tcgccggcca atctcttcgt
      241 cgccgatttc atgggctcgc cgtcgatgaa cctgatcaag gcgaaagtcg cgcgcaacaa
      301 cggcgccgct tcgatcgtcc tcgaacgggc cggcgcggag ccgatcgtca tgcccgcgcc
      361 gacaagcgcc gacgcgacca agctgcgcga cggcggcgcg gtgattttcg gcatcaggcc
      421 cgaagcggtc agcgatccgg aaagcgccga ccgtaactct aagcatgtcg cgaccttccc
      481 ctgccgggtc gatctggtcg agccggccgg ctccgacacg ttcgtcgtga cgtcgatcgc
      541 cggcaaggag gtcacggcgc gcatgcgcgc ggacgccgat gtgcggccgg gacagaacgt
      601 ctccttcgcc ttcaacctcg acaaggcggt ccttttcgat cccgactcga cgcagcgaat
      661 ttgaagacag atctcgctca gcagctcggt gagaggggtc cgacacgctg cggcgtaccc
      721 tcaccccaac cctctcccgc aagcgggaga gggcgcgcgc cgagtgcgtc agcgaagcgc
      781 gggagtgacg aaaaacggaa tcgggcgtgg ggaacccctt ctcccgcttg cgggagaagg
      841 tggcgcgaag cgccggatga gggtgatctg cgaaaagatc gaaaagaggg attctaagag
      901 gcgatgaacg cccaaaggga cgtcgacgtt gccgtcattg gctcaggcat gggcggggcg
      961 agcttcgcgg cgggcctggc gccgtccggc gcgcgcatcg tcattatcga gcgaggcgag
     1021 cgcatcgccg cgagcgcgct ggcgcgcgat ccgcgcgcca tcttccagca cggccatttc
     1081 cggccgcagg aattctggta cgacggcgcc ggccgtccgt tcaatcccgg caattactat
     1141 tatgtcggcg gcaacaccaa gctttatggc gccgttctgc tccgctatcg cgccgaagac
     1201 ttccgcccga tcgaatatgc ggagggcgcg acgccgggtt ggccgttccc ttacgaagag
     1261 ctggagccgt ggtacagccg cgcggaggcg ctctaccagg cgcgcggcgc gctcggcgaa
     1321 gatccgaccg agccgcccca ttccaagccc tatccttttc cgccggtgcc ggacgagccg
     1381 gccatcgccg cgttgcgcca gcggttcaag cagatcggcc ttcacccgtt ctcgctgccg
     1441 ttaggcgtcg atatcgaggc gtggctgaaa ggcggccaga cgccgtggga cgcgtttccg
     1501 aacacggatt ccggcaagat ggacgccgag acctgcggcc tcgccgcggc gctcaaacac
     1561 gaaaacgtgt cgctggagac gggcgcgcgc gtcacgcgcc tcgaaaccga tcccgccggc
     1621 aaacggatcg agagcgtcgt ctatcaaaag aacggcgaga cgttaaagct ctcgccgaag
     1681 gcggtcgtgc tcgccgccgg cgcggtgaat tcggcgctgc tcctgctcgc ctcggccaat
     1741 ggcgcgatgc cgagcggcct cgccaaccgc tccgatcagg tcggccgcca tttcatgaac
     1801 cacaacctct cgttcgctct ggcgcttcac ccgttgcgaa agaacgactc cgtctatcaa
     1861 aagacgatcg gcctgaacga tttctacctc agcgacggac gcggcgggcc gccgctcggc
     1921 aatgtgcaaa tggtcggcaa gatttccggg ccgatcctta aatccgaatt gcgccgcgcg
     1981 ccggaaacgg cgctcggcgt cctggcgcga cgcacattcg acttcctgtt catgagcgag
     2041 gatctgccct cgccggagag ccgcgtccgg ctcgacggct cgcgcgtcgt gctcgactgg
     2101 cggcgctcca atatcgcggc gcataacgga ctggtcgtgc gaatgcgcga aatgctcaaa
     2161 gaggccggct ttccgatcgt cctcgtcaaa gccgtcgatc
//
LOCUS       DATGJW010000236         7912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_251554, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000236 DATGJW010000000
VERSION     DATGJW010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7912
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_251554"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            314..1168
                     /locus_tag="VKU03_07320"
     CDS             314..1168
                     /locus_tag="VKU03_07320"
                     /inference="COORDINATES: protein motif:HMM:NF016974.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0018193 - peptidyl-amino acid modification
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/asparaginyl beta-hydroxylase
                     domain-containing protein"
                     /protein_id="HLJ71110.1"
                     /translation="MSSTSLDTRTGKDRVARFAKIALRRIAAPIVGLAAFIYFLPKIA
                     LLYAVTGAYDVTRNRGLTVATVRRYLFGNGFLTWLLSPLNALLDILSLPYVNKGVYTP
                     EDLPPAYRDEVRRLIQIAKDENLVAQLEQRVRDNARTMIFFRWYGANIDTFLDIPAFH
                     QDWRYIQTIGVSVFNKKVSTSWHFGFTRASLRVLYNLNDITDRTAFIQCNGATHYWAE
                     EKLYIFDDTLLHRSVNETAQTRYCLFVDIIRPTPLPAVMRFVVSAIALLTKSFKFVYY
                     KNWKVIER"
     gene            complement(1271..1507)
                     /locus_tag="VKU03_07325"
     CDS             complement(1271..1507)
                     /locus_tag="VKU03_07325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71111.1"
                     /translation="MPDQRPVNWRNALTLFSIAILVGTELVGAGASAGWAIGGLFQLG
                     GLFTHVLEVIFVLCALVGLSYFVRAALAHEPIRG"
     gene            1663..3246
                     /locus_tag="VKU03_07330"
     CDS             1663..3246
                     /locus_tag="VKU03_07330"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015821618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HLJ71112.1"
                     /translation="MSDTPTFSRAAVAAPHRLAAEAGRAILAERGNAIEAMVAMAAAI
                     AIVYPHMNSIGGDGFWLIREQGGRARAIEACGFAGQGATMERYRRLGAIPCRGPEAAL
                     TLPGTIGGWALALELSARLGGKLPLAVLLDGAIRAAREGYAVSRSEARAAPFEFERLK
                     AAPGFAATYLVDGKPAEAGAIRTAPALAETLAYLAHAGLDDFYRGDIGREIAADLQRI
                     GAPIGRDDLRAYRAAWREPLSLRLSDATLYNTPPPTQGLASLILLGLFERLDIKRAGD
                     FAYFHALIEASRRALSIRDRVCADFDHLAEDPADHLTAAALDRDAAAIDMSRAAPWPA
                     RRDKGDTIWMGAIDDQGLAVSFIQSIYWEYGSGCVLPATGVLLSNRGLAFSLDPKSPN
                     ALQPGRRPPHTLNPPLAAFDDGRVATYGAMGGDGQPQFQAQVFTRYRFGEDIAAALAA
                     PRFLAGRAWGAPSPTVKLEGGFDETIGRALAAAGHPIEWMQAPFADAFGHAGMVVRDA
                     KGAIAAAHDPRADGGAAGM"
     gene            3526..4953
                     /locus_tag="VKU03_07335"
     CDS             3526..4953
                     /locus_tag="VKU03_07335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HLJ71113.1"
                     /translation="MTEWPRHGRIDGPIVMIGFGSIGKGTLPLIERHFDYDKSRFIVI
                     DPDDSDRALLDERGVAFLKLAITRDNYREVLTKYLAGGRQGFCVNLSVDTSSLALMEH
                     CREIGALYIDTVVEPWPGFYFDKSLGPETRSNYALRETVLEARRRKPGGATAVSCCGA
                     NPGMVSWLVKQALINLAADLGDKGAEPKGKEEWGRLAQRLGVKGIHIAERDTQRAKQP
                     KPRNVFVNTWSVEGFLSEGMQPAELGWGTHEKWAPSNAGFHKTGCGAAIYLNQPGANT
                     RVRSWTPTALGQYGFLVTHNESISIADYFTLNGDGRATYRPTCHYAYHPCDDAVLSLH
                     ELFGRAGVAPETHQILGEKEILDGIDELGVLLYGHARGAYWYGSQLSIEETRAIAPYQ
                     NATGLQVTSAVLAGMAWALENPNAGIVEADEMDFRRCLEVQRPYLGPVVGAYTDWTPL
                     KDRPGLFPEDIDESDPWQFRNVLVR"
     gene            5156..6001
                     /gene="dapD"
                     /locus_tag="VKU03_07340"
     CDS             5156..6001
                     /gene="dapD"
                     /locus_tag="VKU03_07340"
                     /EC_number="2.3.1.117"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008666 -
                     2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3,4,5-tetrahydropyridine-2,6-dicarboxylate
                     N-succinyltransferase"
                     /protein_id="HLJ71114.1"
                     /translation="MSLQRLQSVVEQAFEDRAAINAQTKGDIRDAVEKALAMLDSGEA
                     RVAEKSADGGWKVNQWLKKAVLLSFRLNDSAPISGGPGGAHWYDKVESKFADWGADRF
                     AAAGFRAVPGAIVRHSAFIAPGAVLMPSFVNVGAYVGANTMVDTWVTVGSCAQIGKNV
                     HLSGGVGIGGVLEPLQANPTIVEDNCFIGARSEIVEGVIVGEGSVVSMGVFIGASTKI
                     VDRSTGKVHVGRVPPYSVVVSGSLPGKNLPDGAAGPSLYCAVIVKTVDAQTRAKTGIN
                     ELLRD"
     gene            6010..6483
                     /locus_tag="VKU03_07345"
     CDS             6010..6483
                     /locus_tag="VKU03_07345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71115.1"
                     /translation="MTKHRLNFLYRRADGAIGRREWALASLVPTIVIVVLSLIWAMIM
                     PRQERDLSQGLVDLGVAVRYLYLIVYAFIVLICAVAQYFVSAKRFVDLGKPPALAGAA
                     PFALFFAAAANWYEPRSEGLAPHWAILALDAVALAAVAWNIAELGFVKGRSASSQ"
     gene            6736..7137
                     /locus_tag="VKU03_07350"
     CDS             6736..7137
                     /locus_tag="VKU03_07350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71116.1"
                     /translation="MPVVSKPRRAKSIAAAPAKAGKPVPRKASATHRKAAAAAPADFA
                     QQQVARLEAMFDRLAEKANQGELGAIDRSLKILDRLDRYQGFSPAAAPEESDEDARER
                     ILRILSDVDARRAAAAQEEEGEGADDAPADA"
     gene            7115..>7912
                     /locus_tag="VKU03_07355"
     CDS             7115..>7912
                     /locus_tag="VKU03_07355"
                     /inference="COORDINATES: protein motif:HMM:NF015211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="terminase family protein"
                     /protein_id="HLJ71117.1"
                     /translation="MTRRPTPDAGRIVIRHVRDLIALKPVERLRRLAQLTAADCDRLL
                     HDWPLWARPDQLPPPGEWVAWLILAGRGAGKTRAGAEAVRHWIQNYSPVNLIGATAAD
                     VRDVMVLGESGLLACCRAAERPVYARTARRLTWPNGGVSLLFSAEEPDRLRGEQHAKL
                     WCDELAAWRYPDTYDQALLGLRLGDRPQVVVTTTPRPIKLVKDLLGDPNAVVTRGVTF
                     DNRDFLARAFVKRIAERYSGSRLGQQEIYAEILEETPGALWTRDLLER"
BASE COUNT         1289 a         2667 c         2636 g         1320 t
ORIGIN      
        1 caaaacgtct tggtagccaa taagccacgc cggacgatcg cagtcacccg aagggccgga
       61 acgaagtgaa ggacccgcca tagcgccgca cgatcgggcg ttcgttcgcg cgtaactcgg
      121 gaggcgctat ggcgggttga cggcgatcgg ccggcgtggc gccgcggcgc tgaaaggtta
      181 cgtctcagac gccgtgacaa cctcagactt tccccggaca gccctgcgca agaggcgggg
      241 agaagggata cccatcgcgg cggatcggtg ctaaaccccg gcccgaaagc cgggccgcgg
      301 agagagcatt cccttgagca gcacttccct cgacacgcgg acgggcaaag atcgcgtcgc
      361 ccggttcgcg aaaatcgcct tgcgccggat cgctgcgccg attgtcgggc tcgcggcgtt
      421 catctacttc ctgccgaaaa tcgcgctcct ctacgccgtc accggcgcct atgacgtgac
      481 ccgcaatcgc ggcctgacgg tcgcgaccgt caggcgctat ctgttcggca acggcttttt
      541 gacctggctc ctgtcgccgc tcaacgcgct gctcgacatt ctctcgctgc cttatgtgaa
      601 caagggcgtc tacacgcccg aagacctgcc gcccgcctac cgggacgagg tcaggcgcct
      661 gatccagatc gccaaggacg agaatctcgt cgcgcaattg gagcagcgcg tgcgcgacaa
      721 cgcccgcacc atgatcttct tccgctggta cggcgccaat atcgacacgt tcctcgatat
      781 tcccgccttc catcaggatt ggcgctacat ccagacgatc ggcgtctcgg tcttcaacaa
      841 gaaggtgtcg acctcctggc atttcggctt cacccgcgcg tccctgcgcg tgctctataa
      901 cctcaacgac atcaccgacc gcacggcgtt catccaatgc aacggcgcga cccattattg
      961 ggccgaagag aaattgtaca ttttcgacga tacgctgctg catcgctcgg tcaacgagac
     1021 ggcgcagacg cgctattgcc tgttcgtcga catcattcgc ccgacgccgc ttcccgccgt
     1081 gatgcgcttc gtcgtctcag cgatcgcgct gctgaccaag agcttcaagt tcgtgtacta
     1141 taagaactgg aaggtcatcg aacggtgaga gtcctctccc agaggggaga gggacagaga
     1201 agcggcggcg cattttgcgc tcacataaag ccgggccctg accctctcgg ggagaggaga
     1261 ggcgcgattc tcacccgcgg atcggctcgt gcgcgagggc ggcgcggacg aaataggaaa
     1321 ggccgaccag cgcgcagagg acgaaaatca cttcgagaac gtgggtgaag agcccgccga
     1381 gttgaaacag gccgccgatc gcccagcccg ccgacgcgcc ggccccgacc agttcggtgc
     1441 cgaccaggat ggcgatgctg aacagggtga gcgcgttgcg ccaattgacg ggccgctggt
     1501 caggcaaagg ggactcccgg actctccttg ccggacctat acgatcgaag ggggaagggc
     1561 aagccggccg gcgccaagcc taacgattgc gcaaaggatg cgctggcccg gcaaggccgc
     1621 gttctgctat ggggccgccg gcgacaggac gccgagcgag cgatgagcga cacgccgacc
     1681 ttctcccgcg ccgccgtcgc ggcgccgcat cgcctcgccg ccgaagcggg gcgggcgatc
     1741 ctcgccgagc gcggcaatgc gatcgaagcg atggtcgcga tggcggcggc gatcgcaatt
     1801 gtctatccgc atatgaactc gatcggcggc gacggcttct ggctgatccg cgagcaaggg
     1861 ggccgcgcgc gggcgatcga ggcctgcggc ttcgccggcc agggcgcgac tatggagcgc
     1921 tatcgccgtc ttggcgcaat cccgtgccgc gggccggaag cggcgctgac cttgccgggg
     1981 acgatcggcg gctgggcgct ggctttggag ctttcggcgc gtttgggcgg caagctgccc
     2041 ctcgccgtgc tgctcgacgg cgcgatccgc gcggcgcgcg agggctatgc ggtctctcgg
     2101 tccgaggccc gcgcggcgcc gttcgagttc gagcgcctca aggcggcgcc gggcttcgcc
     2161 gcgacgtacc ttgtcgatgg caagccggcc gaggcgggcg cgatccgcac ggcgccggct
     2221 ctcgccgaga cgctggccta tctcgcccat gccgggctcg acgatttcta ccgcggcgat
     2281 atcggccgcg aaatcgccgc cgacctgcag cggatcggcg cgccgatcgg ccgcgacgac
     2341 cttcgcgcct accgcgccgc gtggcgcgag ccgctgtcgc tgcggctttc cgacgcgacg
     2401 ctctacaaca cgccgccgcc gacgcaaggc ctcgcctcgc tcatcctgct cggcctgttc
     2461 gagcggctcg acatcaagcg cgccggcgat ttcgcctatt ttcacgctct catcgaggcc
     2521 tcgcgccgcg cgctttcgat tcgcgaccgc gtctgcgcgg atttcgacca tctggcggag
     2581 gatccggccg atcatctgac ggcggccgcg ctcgatcgcg acgcggcggc gatcgacatg
     2641 agccgcgccg cgccgtggcc ggcgcggcgt gacaaaggcg atacgatctg gatgggcgcg
     2701 atcgacgatc aaggcctcgc cgtctcgttc atccagtcga tctattggga atatggctcg
     2761 ggctgcgtcc tgccggcgac aggcgtgctg ctcagcaatc gcggtctcgc cttctcgctc
     2821 gatccgaaat cgccgaacgc gctgcagccg ggccggcggc cgccccatac gctcaatccg
     2881 ccgctcgccg cgttcgacga cggccgcgtc gcgacgtacg gcgcgatggg cggcgacggc
     2941 cagccgcaat tccaggcgca ggtcttcacc cgctatcgct tcggcgagga catcgccgcc
     3001 gcgctcgccg cgccgcgctt cctcgccggc cgcgcttggg gcgcgccgtc gccgaccgtg
     3061 aagctcgaag gcggcttcga tgagacgatc ggccgcgctt tagcggcggc gggccatccg
     3121 atcgaatgga tgcaagcgcc gttcgccgac gcgttcggcc atgccggcat ggtcgtgcgc
     3181 gacgctaaag gcgcgatcgc cgccgcccac gatccgcgcg ccgacggcgg cgcggcgggg
     3241 atgtagttga aggggcgggg caaggccgcg gctcgcgctc atcccgcggc ttcgccgccg
     3301 ccttctcccg caagcgggag aaaagaatct gtagccgcgg tcggcgaccg cggctacagc
     3361 gcgccgactg atgaaggcgg cggcgcgccc ctttcccgct tgcgggagag agctcgggcg
     3421 agggtaagcc gggggagaag ggttgacggg cgcggccgcg cttgctctta agccgcgccg
     3481 gcgatttccg ccgcgctcga tctctaaccg cttcgggagc aggcgatgac ggaatggccg
     3541 cggcatgggc ggatcgacgg cccgatcgtg atgatcggct tcggttcgat cggcaaaggg
     3601 accttgccgc tgatcgagcg ccatttcgat tacgacaaga gccggttcat cgtcatcgat
     3661 cccgacgatt cggaccgcgc gctgctcgac gagcgcggcg tcgccttcct caaactggcg
     3721 atcacccggg acaattatcg cgaggtcctg accaaatacc tggcgggcgg ccgccagggc
     3781 ttttgcgtga atctctcggt cgatacctcg tcgctggcgc tgatggagca ttgccgcgag
     3841 atcggcgcgc tctatatcga cacggtggtc gagccctggc ccggcttcta cttcgacaag
     3901 agcctcggcc cggagacgcg ctcgaattac gctctgcgcg aaacggttct cgaggcgcgg
     3961 cgccgcaagc cgggcggcgc gacggcggtc tcctgctgcg gcgccaatcc cggcatggtg
     4021 tcgtggctcg tcaagcaggc cttgatcaac ctcgccgccg atctcggcga caagggcgcc
     4081 gagccgaagg gcaaggagga gtggggcagg ctcgcccagc gcctcggcgt caagggaatc
     4141 catatcgccg agcgcgacac gcagcgcgcc aagcagccga agccgcgcaa cgtcttcgtc
     4201 aacacctggt cggtcgaggg ctttctctcc gagggcatgc agccggccga gctcggctgg
     4261 ggcacgcatg agaaatgggc gccgagcaat gccggcttcc acaagacggg ctgcggcgcg
     4321 gcgatctatc tcaaccagcc gggcgccaat acgcgcgtgc gctcgtggac gccgacggcg
     4381 ctcggccaat acgggtttct cgtcacgcat aacgaatcga tctcgatcgc cgactatttc
     4441 acgttgaacg gcgacggccg ggcgacgtac cgcccgacct gccattacgc ctatcaccct
     4501 tgcgacgatg cggtcctgtc gctgcatgag ctcttcggcc gcgccggcgt ggcgccggag
     4561 acccaccaga ttctcggcga gaaggagatt ctcgacggga tcgacgagct cggcgtgctg
     4621 ctctacggcc atgcgcgggg cgcttattgg tacggctcgc agctctcgat cgaggagacc
     4681 cgcgccatcg cgccttatca gaacgccacc ggcctgcaag tgacctcggc cgttctcgcc
     4741 ggcatggcct gggcgctgga gaatccgaat gccggcattg tcgaggcgga cgagatggat
     4801 ttccgccgct gccttgaagt gcagcggccc tatcttgggc cggtggtcgg cgcctatacc
     4861 gactggacgc cgctcaagga ccggccgggc ctgttccccg aggatatcga cgagagcgac
     4921 ccctggcagt tcaggaatgt actggtaagg tgacaatccc ttctccccgc tccgcgggga
     4981 gaaggtggcc cgaagggccg gatgaggggc tgcgcaggac cagaaagtac gaacaccgga
     5041 ctctcgctcg aagctcagtc gctcctcgtc aatcgccgcg cagcccccga cccggccgct
     5101 tcgctgccac cctctccccg ctgcggcggg cgaggggaat gaaaaggaac cgcccatgtc
     5161 cctccagcgt cttcaatccg tcgtcgagca ggccttcgag gatcgcgcgg cgatcaacgc
     5221 gcaaaccaag ggcgacattc gcgacgcggt ggagaaagcg ctcgccatgc tcgattccgg
     5281 cgaggcgcgc gtcgccgaga agagcgccga cggcggttgg aaggtgaatc aatggctcaa
     5341 gaaagccgtg ctcctgtcct tccgcctcaa tgacagcgcg ccgatttcgg gcgggccggg
     5401 cggcgcgcat tggtacgaca aggtggagtc gaaattcgcc gattggggcg ccgaccgctt
     5461 cgccgcggcg ggcttccgcg ccgtgcccgg cgcgatcgtg cgccattcgg ccttcatcgc
     5521 gccgggcgcg gtgctgatgc cgtccttcgt caatgtcggc gcctatgtcg gcgccaacac
     5581 gatggtcgac acctgggtca cggtcggctc ctgcgcccag atcggcaaga acgtgcacct
     5641 ttccggcggc gtcggcattg gcggcgtgct cgaaccgttg caggccaatc cgaccatcgt
     5701 cgaggacaat tgcttcatcg gcgcgcgctc cgaaatcgtc gaaggcgtga tcgtcggcga
     5761 gggctcggtc gtctcgatgg gcgtgttcat cggcgcctcg accaagatcg tcgaccgctc
     5821 gaccggcaag gttcatgtcg gccgcgtgcc gccttattcg gtcgtcgtct ccggctcgct
     5881 gcccggcaag aacctgcccg acggcgcggc cggcccgtcg ctctattgcg cggtgatcgt
     5941 caagacggtc gacgcgcaga cccgcgccaa gaccggcatc aacgaactgc tgcgcgattg
     6001 atgcaagctt tgacgaaaca tcgcctgaac tttctctatc gccgcgccga cggcgccatc
     6061 ggccggcgcg aatgggcgct cgccagcttg gtcccaacga ttgtcatcgt cgtgctctcc
     6121 ctgatctggg ccatgatcat gccgcgccag gagcgcgacc tttcgcaagg gctggtcgat
     6181 ctcggcgtcg ccgtgcgcta tttgtacctc attgtctatg cgttcatcgt gctgatctgc
     6241 gcggtcgcgc agtatttcgt cagcgccaaa agatttgtcg atctcggcaa gccgccggcc
     6301 ttggcgggcg ccgcgccttt cgcgctgttc ttcgccgcag ccgccaattg gtacgagccg
     6361 cgctccgaag gcttggcgcc gcattgggcg attctggcgc tcgatgcggt cgcgctcgcg
     6421 gcggtggcgt ggaatattgc cgagctggga tttgtgaagg gccgcagcgc ttcctcgcaa
     6481 tgagtggtct tgtttgtaag ttattggcat catgtaataa atttccgtaa ttgctgattt
     6541 tcagaaacaa aagcttgcgc gtttacgcga ctagccgcat aatcagccac agtggagatg
     6601 gagcgagttg agcccgttgc gggcgccgtc tcccgcaata acaagatcgt ccttcgtcca
     6661 acccttggcg atgtcctccg accgccggga cgcgcccctt cgcgctattc catccacatt
     6721 tgacatggag ccgccatgcc cgtcgtcagc aagccgcgcc gcgctaagag cattgcggcc
     6781 gcgccggcga aggccggcaa gcccgtcccg cgcaaggctt ccgccacgca tcggaaggcc
     6841 gcggcggctg cgcccgccga tttcgcgcag cagcaggtcg cccggctgga ggcaatgttc
     6901 gaccgcctgg ccgagaaggc gaatcagggc gaacttggcg cgatcgatcg cagcttgaag
     6961 atcctcgacc ggctcgaccg ctatcaggga ttttcgcccg cagcggcgcc ggaggaatcc
     7021 gacgaggatg cgcgcgagcg catcttgcgc atcctttccg acgtcgacgc gcggcgcgcc
     7081 gcggccgcgc aagaggaaga aggcgaggga gcggatgacg cgccggccga cgcctgacgc
     7141 cggccgcatc gtcattcgcc acgtccgcga tctgatcgcg ctcaagccgg tcgagcggtt
     7201 gcggcggctg gcgcagctga ccgcggccga ttgcgaccgc ctgctccacg actggccgct
     7261 ctgggcgcgg ccggatcaat tgccgccgcc gggcgaatgg gtggcgtggc tgatcctcgc
     7321 cggccgcggc gccggcaaga cgcgcgccgg cgccgaggcg gtgcgccatt ggattcagaa
     7381 ttactcgccg gtcaacctga tcggcgcgac cgcggcggat gtgcgcgacg tcatggtgct
     7441 cggcgaatcg ggcctgctcg cctgctgccg cgctgcggag cggccggtct atgcgcgcac
     7501 ggcgcggcgc ctgacctggc cgaatggcgg cgtgtcgctg ctcttctcgg ccgaggagcc
     7561 ggaccggctg cgcggcgagc agcacgccaa attgtggtgc gacgaattgg cggcgtggcg
     7621 ctaccccgat acgtacgatc aggctttgct cggcttgcgg ctgggcgacc ggccgcaagt
     7681 cgtggtcacg acgacgccgc ggccgatcaa gctcgtcaag gacctactcg gggatcccaa
     7741 cgccgtggtg acgcgcggcg tcaccttcga caatcgcgac tttctcgccc gcgccttcgt
     7801 caagcgaatc gccgagcgct atagcggcag ccgcctcggc cagcaggaaa tctacgccga
     7861 gattctcgag gagacgccgg gcgcgctatg gacgcgcgac ctgctcgagc gc
//
LOCUS       DATGJW010000237         3196 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_874081, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000237 DATGJW010000000
VERSION     DATGJW010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3196)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3196)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3196
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_874081"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            2..1303
                     /locus_tag="VKU03_07360"
     CDS             2..1303
                     /locus_tag="VKU03_07360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase/cysteine synthase family
                     protein"
                     /protein_id="HLJ71118.1"
                     /translation="MRNETLSIHGGFVCDPATKAVAVPIYQSVAYSFDSADHGAALFN
                     LETDGFRYSRISNPTTEILERRVAALEGGLAAISAASGQAALNYAFLTLADCGGNIVA
                     PPTLYGTTHTVLAHNLRRQGVEARFAANDSAAAIEGLIDENTRAVFCESIGNPAGNIC
                     DIEALAKAAHRHGVPLIVDNTVATPILLRPIEYGADIVVHSLTKFMGGHGAAMGGAVV
                     DSGAFDWTAQAKRFPMFSQPDESYHGLVYSERFGREAFIARCRSVYQRTMGAVLAPLS
                     AFLLLQGIETVALRMERHVENGRKVAEFLRGDPRVAWVNYAGFPDSPYYALAQKYLRG
                     RASSLLTFGLHGGLEAGKAFYDALNLVKRLVNIGDAKSLACHPASTTHRQMTLAEQAK
                     AGVRPEMIRMSVGIEHIDDILEDLDQALAAAASRRPAVAAE"
     gene            1312..2358
                     /locus_tag="VKU03_07365"
     CDS             1312..2358
                     /locus_tag="VKU03_07365"
                     /EC_number="2.3.1.46"
                     /inference="COORDINATES: protein motif:HMM:NF003776.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homoserine O-succinyltransferase"
                     /protein_id="HLJ71119.1"
                     /translation="MPVTIERPALAAAARISDLRERRRRRGTIRDRGRRLAIGLVNNM
                     PDSALAATERQFRTVLEQASGDLDIQLRLYALPQVPRAREALEHVAQLYADAAALRGQ
                     GADALIVTGAQPKAPDLKDEPYWRSLSAVIDWAAGNTLSTILSCLAAHVGVRHFSGIQ
                     RRALPRKCSGVFTFERVGADRLTEGADAAWLTPHSRYNGLDERELTRNGYTVLTRSPS
                     AGVDMFVKRMRSLVVFLQGHPEYESDTLAREYRRDLSRRLAGDSALLPDIPEGYFSPE
                     AERALREFSARALRELKPDAMAFYPHIGLSGASDAPWRRSANLLYRNWLGLVAERKAE
                     IVQGSSSLAARWGG"
     gene            2367..2903
                     /locus_tag="VKU03_07370"
     CDS             2367..2903
                     /locus_tag="VKU03_07370"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLJ71120.1"
                     /translation="MAYLLRQPIEEAAAAPRAAQPTAGEAFRSAMREFASGVAIVAAG
                     QGERRNGCTATSFCSLSIDPPSLVVCLDRASATLATLRSEGAFGVSMLAEAHADLADR
                     FAGRNGLRGAARFAGAGWTALSSGAPLLQGAVATLDCAVEEIVERHTHAIVIGRVIAA
                     RAWGGRPLLHWRGRALSP"
     gene            2915..>3196
                     /locus_tag="VKU03_07375"
     CDS             2915..>3196
                     /locus_tag="VKU03_07375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HLJ71121.1"
                     /translation="MSDSSPLRERTPSGGGRSAEPKPAEIWRRALAATARLGGEPARI
                     LPAVVNEFAQTHGENPALLSDRETFSFAALADRMNRYSRWALGQGIAAGD"
BASE COUNT          497 a         1089 c         1050 g          560 t
ORIGIN      
        1 gatgcgtaat gagacacttt cgatccatgg cggcttcgtc tgcgacccgg ccaccaaggc
       61 ggtcgccgtg ccgatctatc aatcggtcgc ctattctttc gacagcgccg atcatggcgc
      121 ggccctgttc aatctcgaga ccgacggctt ccgctatagc cgcatcagca atccgaccac
      181 cgagattttg gagcgccgcg tcgccgcgct cgaaggcggg ctcgccgcca tttccgccgc
      241 ctcgggtcag gcggcgctga actacgcctt cctcacgctc gccgattgcg gcggcaatat
      301 cgtcgcgccg ccgacgcttt atgggacgac gcatacggtg ctcgcgcata acctgcgccg
      361 gcagggcgtc gaggcgcgct tcgccgcgaa cgatagcgcc gcggcgatcg agggcctgat
      421 cgacgagaac acccgcgccg tcttctgcga aagcatcggc aatccggccg gcaatatctg
      481 cgacatcgag gcgctggcca aggctgcgca tcgtcacggc gttccgctga tcgtcgacaa
      541 tacggtggcg acgccgatcc tgctgcggcc gatcgaatac ggcgccgaca tcgtcgtcca
      601 ttcgctgacc aaattcatgg gcggccacgg cgccgcgatg ggcggcgcgg tcgtcgatag
      661 cggcgccttc gactggacgg cgcaggccaa gcgctttccg atgttcagcc agccggacga
      721 atcctatcac ggcctggttt attccgagcg tttcggccgc gaggcgttca tcgcgcgctg
      781 ccgcagcgtc tatcagcgca cgatgggcgc ggtgctggcg ccgctcagcg cgttcctgct
      841 gctgcaaggg atcgagacgg tcgctctacg catggagcgt catgtcgaaa acggtcgcaa
      901 agtcgccgag tttttgcgcg gcgatccgcg cgtggcgtgg gtgaattacg ccggcttccc
      961 tgacagcccg tactacgcgt tggcgcagaa atatctgcgc ggccgcgcct cctcgcttct
     1021 tactttcgga cttcatggcg ggctcgaagc gggcaaggct ttttatgacg cgctgaacct
     1081 ggtcaaacgc ctggtcaata tcggcgacgc caagtcgctc gcctgccatc cggcttcgac
     1141 cacgcatcgc cagatgacgc ttgccgagca agccaaggcg ggcgttcgcc ccgaaatgat
     1201 ccggatgagc gtcggcatcg aacatatcga cgatattctc gaagacttgg accaagcgct
     1261 cgccgccgcc gcgagccggc gtcccgcggt tgcggcggaa taggcggcga catgcccgtc
     1321 accatcgaac gaccggctct tgccgccgcg gcgcggatca gcgatctgcg cgagcggcgc
     1381 cgccgccgcg gcacgatccg cgatcgcggc cggcgtctcg cgatcgggct cgtcaacaac
     1441 atgccggaca gcgcgctcgc cgcgactgag cgccagttca gaaccgtgtt ggagcaagcc
     1501 tccggcgatc tcgacatcca attgcgcctc tacgcgttgc cgcaagtgcc gcgcgcgcga
     1561 gaggcgctgg agcatgtcgc gcagctctat gccgacgccg ccgctttgcg cggccaaggc
     1621 gccgacgcgc tgatcgtcac cggcgcccag ccaaaagcgc cggatctcaa agacgagccc
     1681 tattggcgca gtttgagcgc cgtgatcgat tgggcggcgg gcaatacgct ctcgaccatc
     1741 ttgtcctgcc tcgccgctca tgtcggcgtt cgccatttca gcgggattca gcgccgcgct
     1801 ttgccgcgca aatgctcggg cgtctttacg ttcgagcgcg tcggcgccga ccgactgacc
     1861 gaaggcgcgg acgcggcgtg gctgacgccg cattcgcgct acaacggact cgacgagcgc
     1921 gagctgacgc gcaacggcta tacagtcttg acgcggtcgc cgagcgccgg cgtggatatg
     1981 tttgtcaagc ggatgcgcag cctcgtcgtt ttcctacaag gtcatccgga atatgagagc
     2041 gacacgctgg cgcgcgaata tcgccgcgat ctgagccggc ggcttgccgg cgattccgct
     2101 ctgcttccag acattcccga gggctatttc tcgccggaag ccgagcgcgc gttgagggaa
     2161 tttagcgccc gcgcgctgcg cgagctcaag cccgacgcca tggcgtttta tccgcatatc
     2221 ggcctgtccg gcgcgtcgga cgcgccgtgg cgtcgttctg caaatctgct ctatcgcaat
     2281 tggctcggcc tcgtggcgga gcgcaaggcg gagatcgtgc aaggctcttc ctcgctcgcg
     2341 gcgcggtggg gcggctgagg cgcaacatgg cctatctgct gagacaaccg atcgaggaag
     2401 ccgcagccgc gccgcgcgcc gcccagccga cagccggcga agcgttccgc tcggcgatgc
     2461 gcgaattcgc cagcggcgtc gcgatcgtcg ccgctggcca aggcgagcgg cgcaatggct
     2521 gcacggcgac ctcgttctgc tcgctctcga tcgatccgcc ttcgctcgtc gtttgtctcg
     2581 accgcgcctc ggcgactctg gcgacattgc ggagcgaagg cgctttcggc gtcagcatgc
     2641 tcgccgaagc gcatgccgat ctcgccgacc gcttcgccgg ccgcaacgga ttgcgcggcg
     2701 cggcgcggtt cgccggcgcg ggttggaccg cgctttcgag cggcgcgccc ttgctgcaag
     2761 gcgccgtcgc cacgctcgat tgcgccgtcg aggagatcgt cgagcgccac acccatgcga
     2821 tcgtgatcgg tcgcgtgatc gcggcgagag cctggggcgg gcggcccttg ctgcattggc
     2881 gcggccgcgc gttgtcgcct taaaagcggc ctcgatgagc gattcatcgc ccttgcgcga
     2941 acgtacgcca tctggcggcg ggcgttcggc cgagccgaag cctgccgaaa tctggcggcg
     3001 cgctttggcg gcgaccgccc gtctcggcgg cgagccggcg cgcattttgc cggccgtcgt
     3061 caacgaattc gcgcaaacgc acggcgaaaa tccggctctg ctctcggacc gcgagacgtt
     3121 cagcttcgcg gcgctcgccg atcgcatgaa ccgctattcg cgctgggcgc tcgggcaggg
     3181 gatagccgcc ggcgac
//
LOCUS       DATGJW010000238         3333 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_44479, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000238 DATGJW010000000
VERSION     DATGJW010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3333)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3333)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3333
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44479"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            226..729
                     /gene="ruvC"
                     /locus_tag="VKU03_07380"
     CDS             226..729
                     /gene="ruvC"
                     /locus_tag="VKU03_07380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008821 - crossover junction DNA
                     endonuclease activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crossover junction endodeoxyribonuclease RuvC"
                     /protein_id="HLJ71122.1"
                     /translation="MGFPAIRILGIDPGLRATGYGLIEQRGSSLAYVLCGSIKTGAER
                     PLAERLAAIHRALTALIEKERPDEAAVEETFVNRDPQSALKLGQARGVALAAPALLGV
                     PVAEYAANVVKKTIVGVGHAEKGQIAMMVKMLLPASPAASADAADALALAITHAQHRG
                     LKRRGAA"
     gene            726..1337
                     /gene="ruvA"
                     /locus_tag="VKU03_07385"
     CDS             726..1337
                     /gene="ruvA"
                     /locus_tag="VKU03_07385"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009379 - Holliday junction helicase
                     complex [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration protein RuvA"
                     /protein_id="HLJ71123.1"
                     /translation="MIGKLKGLVDSIGEGELILDVNGVGYVIAASARTLRNLPAAGQP
                     AVLHVETQMREDSIRLFGFLAEPERDWFRLLQSVQGVGAKVALAILSALSAADLAQAV
                     ALQDKAAVARAQGVGPKLAARIVAELKDKAPLSGAAEMGLPGAAAAAPELGGAARDAV
                     AALVNLGYARPQAMQAVAKSAASLGAAATTSALIKLGLKELAQ"
     gene            1334..2371
                     /gene="ruvB"
                     /locus_tag="VKU03_07390"
     CDS             1334..2371
                     /gene="ruvB"
                     /locus_tag="VKU03_07390"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0009378 - four-way junction helicase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Holliday junction branch migration DNA helicase
                     RuvB"
                     /protein_id="HLJ71124.1"
                     /translation="MTSAPHRLVSPEKRGEDSDASLRPQVLADFVGQEAARRNLKVFI
                     ESARARSEALDHVLFVGPPGLGKTTLAQIMARELGVNFRSTSGPVIAKAGDLAAQLTN
                     LEPRDVLFIDEIHRLNPAIEEILYPAMEDFQLDLIIGAGPAARSVKIDLAHFTLIGAT
                     TRAGLLTTPLRDRFGIPIRLNFYTVEELESIVNRGAQVIGLQLSPDGANEIARRARGT
                     PRIAGRLLRRVRDFAVVEGAKVVDRKIADGALRLLDVDSSGLDQMDRRYLETIALGFG
                     GGPVGVETIAAALSEPRDAIEEIIEPYLIQQALVQRTPRGRVLTGAAFKHLGLKEPAR
                     EAVQAGLFEED"
     gene            2604..2753
                     /locus_tag="VKU03_07395"
     CDS             2604..2753
                     /locus_tag="VKU03_07395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009450789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLJ71125.1"
                     /translation="MPTLFRLLLVLALIAGLVYGAMIALVTYVHVQPREIIQTVPPAK
                     LEKER"
     gene            2750..>3333
                     /locus_tag="VKU03_07400"
     CDS             2750..>3333
                     /locus_tag="VKU03_07400"
                     /inference="COORDINATES: protein motif:HMM:NF014898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific integrase"
                     /protein_id="HLJ71126.1"
                     /translation="MSAPSSSRLVENFLDAIASERAASANTLAAYRRDLSDYVDFLAE
                     NGVSPLEAASEQARAFLAHRAEAGLKASSLARQLSAMRQFHKHLYLEGRRADDPTASI
                     EGPRRNRPVPKILSVADVDRLLGVAQEGPDDPARPSAERLRATRMACLIELLYASGLR
                     VSELLSLPKSAAKARQPLIAIRGKGGKERLVPIS"
BASE COUNT          493 a         1138 c         1134 g          568 t
ORIGIN      
        1 cgcctgagcc gcgcggagcc gtcaaggctc gcgagccatc gcgcggcgcc aactgacttt
       61 ctcctgagcc tgctttggcg agcgacgcgc gaagcgcgct tggccttgac ggcgagcacg
      121 gctcaggcac gccgcgcttc aagatgtgtg catgtcgtag cgctttcgcg gggaaggatt
      181 tcgccaggct ggcggggttg gctggctttg gcgtaatgct tgcccatggg ctttcctgcg
      241 attcgcattc tcggcatcga tccgggcctg cgcgccaccg gctacggcct gatcgagcag
      301 cgcgggtcga gcctcgccta tgtcctttgc ggctcgatca agaccggcgc cgagcggccg
      361 ctcgccgagc gcctcgccgc catccaccgc gcgctgaccg cgctgatcga gaaggagcgg
      421 ccggacgagg cggcggtcga ggagactttc gtcaatcgcg atccgcaatc ggccctgaag
      481 ctcggccagg cgcgcggcgt cgccctcgcc gccccggcgc tgctcggcgt gccggtcgcc
      541 gaatatgcgg ccaacgtggt caagaagacg atcgtcggcg tcggccatgc cgagaagggc
      601 cagatcgcga tgatggtgaa aatgctgctg ccggcttcgc cggccgcctc ggccgacgcc
      661 gccgacgcgc tcgcgctcgc catcacccat gcccagcatc gcggcttgaa gcggcggggc
      721 gcggcgtgat cggcaagctg aaagggctgg tcgattccat cggcgaaggc gaattgatcc
      781 tcgacgtcaa tggcgtcggc tatgtcatcg ccgcctccgc ccgcaccttg cgcaacctgc
      841 cggcggccgg ccagccggcg gtgctgcatg tcgaaaccca gatgcgcgag gattcgatcc
      901 ggctgttcgg cttcctcgcc gaaccggagc gcgactggtt ccgtctgctg cagagcgtgc
      961 aaggggtcgg cgccaaagtg gccctggcga ttttgagcgc gctctcggcc gccgacctcg
     1021 cccaggccgt cgccttgcag gacaaggcgg cggtggcgcg cgcgcagggc gtcgggccga
     1081 agctcgcggc gcgcatcgtc gccgagctga aggacaaggc gccgctctcc ggggcggcgg
     1141 agatggggct gccgggcgcg gccgccgccg cgcccgagct cggcggcgcc gcgcgggacg
     1201 ccgtcgccgc tttggtcaat ctcggctacg ctcggccgca agcgatgcaa gccgtcgcca
     1261 aaagcgcggc ctcgctcggc gcggcggcga cgacttcggc gctgatcaag ctcggactta
     1321 aggagctggc gcaatgacca gcgcgcctca ccgcctcgtt tcgccagaaa agcgcggcga
     1381 agattccgac gcttcgttgc gcccgcaggt cctcgccgat ttcgtcggcc aggaagcggc
     1441 gcggcgcaat ttgaaagtct tcatcgaatc ggcgcgggcg cgcagcgagg cgcttgatca
     1501 tgtccttttc gtcggcccgc ccggcctcgg caagacgacg ctggcccaga tcatggcgcg
     1561 cgagctcggc gtcaatttcc gctcgacgtc cggaccggtc atcgccaagg cgggcgatct
     1621 cgccgcccaa ctcaccaatc tcgaaccgcg cgacgttctg ttcatcgacg agatccatcg
     1681 cctcaatccg gcgatcgagg agatccttta tccggcgatg gaggatttcc agctcgatct
     1741 gatcatcggc gctgggccgg cggcgcgctc ggtgaagatc gacctcgctc atttcacctt
     1801 gatcggcgcg acgactcgcg ccgggctcct gaccaccccc ttgcgcgacc gcttcggcat
     1861 tccgatccgg ctcaatttct acacggtcga ggagctcgaa agcatcgtca atcgcggcgc
     1921 gcaggtgatc ggccttcagc tttcgcccga cggcgccaac gagatcgccc gccgcgcgcg
     1981 cggcacgccg cgcatcgccg ggcgcctgct gcgccgggtg cgcgatttcg ccgtggtcga
     2041 gggcgcgaag gtcgtcgacc gcaagatcgc cgacggcgcc ttgcgcctgc tcgatgtcga
     2101 ttcctctggg ctcgaccaga tggaccggcg ctatctggaa acgatcgcgc tcggctttgg
     2161 cggcgggccg gtcggcgtcg agacgatcgc ggcggcgctt tccgagccgc gcgacgcgat
     2221 cgaggagatc atcgagccct atttgatcca gcaggccttg gtccagcgaa cgccgcgcgg
     2281 ccgcgtcctg accggcgcgg cgttcaagca tctcggcttg aaggagccgg cgcgcgaggc
     2341 ggtccaggcg ggcctgttcg aggaggattg agggcgggtt tcttgggtac gaattgatgc
     2401 ttccaaagtc ggggcggcgg cttaccctca ccccagccct ctcccgcgag cgggagaggg
     2461 ggcgcagcct tcatcggcgc cgttatagcc gccgtcggcg accgcggcca taaccccttc
     2521 tcccgcttgc gggagaaggt ggcgcgaagc gccggatgag ggtaagccgc tgcgttaagt
     2581 actttcgtct tcgaggactg tttttgccga ctctgtttcg cctgctcctg gtcctcgcgc
     2641 tgatcgcggg cctcgtctat ggcgcgatga tcgctctggt gacttacgtg catgtgcagc
     2701 cgcgggagat catccagacg gttccgcccg ccaagctgga gaaggagcga tgagcgcgcc
     2761 gtcgagctcc cgcctcgttg agaatttcct cgacgccatc gcgtcggagc gggcggcgag
     2821 cgccaacacg ctcgccgcct accggcgcga cctttccgac tatgtcgatt tcctcgccga
     2881 aaatggtgtg agcccgcttg aggcggcgag cgagcaggcg cgtgcttttt tggcccatcg
     2941 cgccgaagcg ggattgaaag cctcttcgct ggcgcgccag ctttccgcaa tgcggcaatt
     3001 ccacaagcat ctgtacctcg aaggtcggcg cgccgacgat ccgaccgctt cgatcgaagg
     3061 gccgcggcgc aatcgccccg tcccgaagat cttgagcgtc gccgatgtgg acaggctgct
     3121 cggcgtcgcg caggaagggc ccgacgatcc cgcccggccg agcgccgagc gcctgcgcgc
     3181 gacgcgcatg gcctgcctga tcgagcttct ttatgcgtcg ggcttgcgcg tctccgagct
     3241 tttgagcctg ccgaaaagcg ccgccaaggc gcgccagccg ctgatcgcga ttcgcggcaa
     3301 gggcggcaag gagcggctcg tcccgatttc cga
//
LOCUS       DATGJW010000239         8474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1082049, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000239 DATGJW010000000
VERSION     DATGJW010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8474
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1082049"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VKU03_07405"
     CDS             complement(<1..207)
                     /locus_tag="VKU03_07405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLJ71127.1"
                     /translation="MTAPILDVICIGRSSIDLYGAQVGGRLEDMASFSKAVGGCPTNI
                     AIGTARLGLKSGLITRVGDEHMGRA"
     gene            complement(256..1083)
                     /locus_tag="VKU03_07410"
     CDS             complement(256..1083)
                     /locus_tag="VKU03_07410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007527858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ71128.1"
                     /translation="MATTPQPHDSGTPSLSGKIAIVTGGSQGLGEAIARLFAARGAAG
                     LVMCGRNEKNGKRVAEEIAATGCPTAFVQADLGHVADCRKVVAEADRRFKRVDVLVNA
                     AALTDRGTIFDTTEERFNDIFNVNVRAPFFLIQETAKIMRREKIHGSIVNIQSMSAHG
                     GQPFISAYCASKGALATLTRNAAHSLSKFRIRVNGLNIGWMATPGEDRIQKTYHDAKD
                     NWLEEAAKTRPFGRLIDPNEVARACLYLASDESGLMTGANIDFDQTIVGVGDPPLEP"
     gene            1248..2231
                     /gene="iolG"
                     /locus_tag="VKU03_07415"
     CDS             1248..2231
                     /gene="iolG"
                     /locus_tag="VKU03_07415"
                     /EC_number="1.1.1.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050112 - inositol 2-dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0019310 - inositol catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol 2-dehydrogenase"
                     /protein_id="HLJ71129.1"
                     /translation="MRFGVIGAGRIGKIHAANIAARKDCEVRLIADVDQAAARSLADA
                     TGAKVAGIEAIIAAPDIDAVAICAPTDMHADLIERAARAKKAVFCEKPVDLSADRIRQ
                     CLKVVEAAGAPLMIGFNRRFDPNFAAIRARIAQGAIGAVEIVQITSRDPGPPPLSYIA
                     RSGGLYRDMMIHDFDMARFLLGEEPVAVGAFGAALVDKAIGEAGDIDTAVVILQTKSG
                     KVAEISNSRRATYGYDQRIEVHGAKGMLRAENVHESTVQFAGDHGFSGDKALNFFLER
                     YAGAYRDEIGAFVAAVSSGKAPSPSGEDGLRAQLLADAADQSRRTGQVVRL"
     gene            complement(2293..3759)
                     /locus_tag="VKU03_07420"
     CDS             complement(2293..3759)
                     /locus_tag="VKU03_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71130.1"
                     /translation="MRHFIRAGGFKLRHALIGATALCAVPLAALAGDVAPTSDGAQKL
                     SALFAQYLGKDAASVAVEGDHYAVTVDFAKLLAPLASEGVSIDIAPLKAKLVQQSDGA
                     WRVAQEAYPPAAVKTKDGGFSVHVDGYTFDGLFDPALYGFRSAEVKMEKANVGIQTPE
                     ADETITFGPAHMTGTGAAANGGGFSGDWREEAGQMTMLVNPKKADGPQPDPVSIKAGG
                     LSFNVTLDNMRMREALDLWAFLVAHPSRAALAADEDALKAKLRGLIPLADKFGEDIAA
                     QNIEVDTPKGPVKLGDFKMRFGADKFPAKGDTEIRFAAGSIAPPPGIIPQGLSDLVPT
                     AIDIDIKYGGYDYAAAAEEAISDMHLAGEGPVISDADRPKIQAKLTGGGPLVFTILPS
                     HIVAPQLDLAVEGAFQVLGARPAGKVTVKARNFDNTFAAIKGAGQQIATPQVIAALTL
                     AKGLGKSGADGSLTWVAEYGADGAIKVNGLALGKAPAH"
     gene            complement(3934..4839)
                     /gene="rpoH"
                     /locus_tag="VKU03_07425"
     CDS             complement(3934..4839)
                     /gene="rpoH"
                     /locus_tag="VKU03_07425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor RpoH"
                     /protein_id="HLJ71131.1"
                     /translation="MAAALPVIAGESGLSRYLSEIKRFPMLEPQDEYMLAKRWRDHGD
                     RDAAHKLVTSHLRLVAKIAMGYRGYGLPIGEVISEGNVGLMQAVKRFEPDKGFRLATY
                     AMWWIRASIQEYILRSWSLVKMGTTASQKKLFFNLRKAKSRISALDEGDLKPDQVAQI
                     AHRLGVPEQDVVDMNRRLGGDASLNAPLREEGEGEWQDWLADDQADQESALADREESN
                     TRLDALKSALSVLNPRERRIFEARRLADEPVTLEELSAEFGVSRERVRQIEVRAFEKV
                     QAAVKAGVARAQLPHSSSPAALPAM"
     gene            complement(5009..6013)
                     /locus_tag="VKU03_07430"
     CDS             complement(5009..6013)
                     /locus_tag="VKU03_07430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HLJ71132.1"
                     /translation="MLPAGEPLAFTVAPGAPGERLDRYLARAAAESDLALSRTRLKAL
                     IEAGEVAVNGAPLRDPAAKLSAGDRVALRVPAPEPSKLEGEDIALDIVFEDRHLLVLD
                     KPAGLVVHPAAGHAKGTLVHALIAHCGDSLSGIGGVKRPGIVHRLDKNTSGLLVVAKT
                     DAAHRGLAELFADHGRSGSLIREYLALVWGAMGRPAGTIDAPLARHARQREKMAVAAA
                     ESGRRAVTHWRLQRALGPASLIACRLETGRTHQLRVHLAHIGHPLLGDSVYGSGFKSK
                     AAQLSEPAQAALAKLDRQALHAAKLGFAHPISGEILSFESPPPEDFTRLIKALAERDN
                     "
     gene            complement(6015..7004)
                     /gene="bmt"
                     /locus_tag="VKU03_07435"
     CDS             complement(6015..7004)
                     /gene="bmt"
                     /locus_tag="VKU03_07435"
                     /EC_number="2.1.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF005718.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="betaine--homocysteine S-methyltransferase"
                     /protein_id="HLJ71133.1"
                     /translation="MFESLLATGRPLLADGATGTNLFAMGLSSGESPESWNDSQRAKV
                     LALHRAFVEAGADIILTNSFGANRRRLMLHGLEGRVGELNRRAAEIARETADRAGRPV
                     AIAGSVGPTGDLLAPLGPLSEEEAVAVFAEQIEGLKAGGADLVWIETMSAPEEMRAAA
                     KAAGALGMPYTVTASFDTAGKTMMGLAPEALAELTESFAPSPLAFGANCGVGASDLLV
                     SILAMTEKRPDAIVIAKANAGIPQWRGAHIHYSGTPALMADYARLAADSGARIIGGCC
                     GTTADHLSAMRRALDERVPGGRPSEAEIVAALGPLASPPAKAHAPRERERRRR"
     gene            7362..7571
                     /locus_tag="VKU03_07440"
     CDS             7362..7571
                     /locus_tag="VKU03_07440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0001072 - transcription antitermination
                     factor activity, RNA binding [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cold-shock protein"
                     /protein_id="HLJ71134.1"
                     /translation="MATGTVKWFNATKGYGFIQPDDGGKDVFVHVSAVERAGMRDLVE
                     GQKVAYEVVADKRTGKSSAGNLRAG"
     gene            7690..>8474
                     /locus_tag="VKU03_07445"
     CDS             7690..>8474
                     /locus_tag="VKU03_07445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HLJ71135.1"
                     /translation="MAGGTFLGLDIGTSSVKAVLIDENQHLIAESAPALSVSRPHPLW
                     SEQNPHDWTQAVEDAVAAIRRQAGAAFSRLAGIGLSGQMHGAVLLGKDDRVLRPAILW
                     NDGRSFAECVELERRAPDSRQRAGNIAMAGFTAPKLLWVAKHEPDVFAATERVLLPKD
                     YVRLFLSGESVSEMSDASGTLWLDVAKRRWDSELLAACGLSEKAMPRLVEGSEVSTSL
                     APGTASRWGLAGRRIPIAGGAGDNAAAAVGIGATGAGQGLVSLG"
BASE COUNT         1389 a         2783 c         2870 g         1432 t
ORIGIN      
        1 cgcgcggccc atatgctcgt cgccgacccg cgtgatgagc ccgctcttga gaccgagccg
       61 cgccgtgccg atggcgatat tggttgggca gccgccgacc gccttcgaga agctcgccat
      121 gtcttcgagc cggccgccga cctgcgcgcc gtaaagatcg atcgacgacc ggccgatgca
      181 gatcacgtcg agaatcgggg cggtcacgac ggatttacct cctgcccttg atcccacaac
      241 gcgcggagcg cacggtcacg gttcgagcgg cgggtcgccg acgccgacga tcgtctggtc
      301 gaaatcgata ttggcgccag tcatcaggcc cgattcgtcg gacgccagat agaggcaggc
      361 gcgggcgact tcgttcggat cgatcagccg gccgaagggc ctggtcttgg ccgcctcttc
      421 gagccaattg tccttggcgt catggtacgt cttctgaatc cggtcctcgc cgggcgtcgc
      481 catccagccg atattgagcc cgttgacgcg gatgcggaac ttactcagcg aatgggcggc
      541 gttgcgggtc agcgtggcga gcgcgccttt cgaggcgcaa taggccgaaa tgaacggctg
      601 gccgccatgg gcggacatgg attggatgtt gacgatcgag ccgtgaatct tctcgcgccg
      661 catgattttc gccgtctcct ggatgaggaa aaacggcgcg cggacattga cgttgaaaat
      721 gtcgttgaag cgctcttcgg tcgtgtcgaa aatcgtgccg cggtcggtga gcgccgcagc
      781 gttgaccagc acatcgacgc gcttgaagcg gcgatcggcc tcggcgacga cttttcggca
      841 atcggcgaca tggccgagat cggcctggac gaaggcggtc gggcagcccg tggccgcgat
      901 ctcctcggcg acgcgcttgc cgttcttctc gttgcggccg cacatgacga ggccggccgc
      961 gccgcgcgcc gcgaacaggc gtgcgatcgc ctcgcccagt ccttgcgagc cgccggtgac
     1021 gatggcgatc ttgccggaaa ggctcggcgt gcccgaatcg tgcggctgcg gcgtcgtcgc
     1081 catgacgttt cctctttgaa cggccagact agttggttca taccaggagc ggaagcgcgg
     1141 gccgcgcagc gcgcctgcgc gggatgaaac aaaaattcta ttcacaagtc aagttggaac
     1201 agttattctc tctctgatcg cgaaattcca ataatgggga ggagaccatg cggtttggcg
     1261 tcatcggcgc gggacggatc ggcaagatcc atgcggcgaa catcgcggcg cgcaaggatt
     1321 gcgaggtgcg cctgatcgcc gatgtcgatc aggccgccgc ccggagcctg gccgacgcga
     1381 cgggcgcgaa agtcgccggg atcgaggcga tcatcgccgc cccggacatc gacgccgtcg
     1441 cgatctgcgc gccgaccgac atgcatgccg atctcatcga gcgcgccgcc agggccaaga
     1501 aggcggtgtt ctgcgagaag ccggtcgatc tcagcgccga ccgcatccgg caatgcctga
     1561 aagtggtcga agccgccggc gctccgctga tgatcggctt caaccgccgc ttcgacccca
     1621 atttcgcggc cattcgcgcc cgcatcgcgc aaggcgcgat cggcgcggtc gaaatcgtcc
     1681 agatcacctc gcgcgatccc gggccgccgc ccctttcgta catcgcccgc tccggcgggc
     1741 tctatcgcga catgatgatc cacgatttcg acatggctcg cttcctgctc ggcgaggagc
     1801 cggtcgcggt cggcgccttc ggcgcggcct tggtcgacaa ggcgatcggc gaggccggcg
     1861 acatcgacac cgccgtcgtg attctgcaga ccaaatccgg caaggtcgcc gaaatctcca
     1921 attcgcgccg cgctacctat ggctacgacc agcgcatcga ggttcatggc gccaaaggca
     1981 tgctgcgcgc cgagaacgtc catgaaagca cggtgcagtt cgccggcgac cacggctttt
     2041 ccggcgacaa ggcgctcaac ttcttcctcg agcgctatgc cggcgcctat cgcgacgaga
     2101 tcggcgcgtt cgtcgccgcg gtctcctccg gcaaggcgcc gagcccgagc ggcgaggacg
     2161 gcctgcgcgc gcaattgctc gccgacgccg ccgatcaatc gcggcgcacc gggcaggtgg
     2221 tgaggctgta acggagcaaa ttccgggcgg tgcggccctt ggagccgcgg tcgtcgaccg
     2281 cgcctccatg ctctaatgcg ccggcgcctt gcccagcgcc agcccgttga ccttgatcgc
     2341 gccgtcggcg ccgtattcgg cgacccaggt cagcgatccg tcggcgccgc tcttgccgag
     2401 gcctttggcg agggtcagcg cggcgatcac ttgcggggtg gcgatctgct ggccggcgcc
     2461 tttgatcgcg gcgaacgtgt tgtcgaaatt gcgcgccttg accgtcactt tgccggccgg
     2521 gcgggcgccc agcacctgga aggcgccctc caccgccaga tcgagctggg gcgcgacgat
     2581 atgcgagggc aggatggtga agacgagcgg tccgccgccg gtcagcttgg cctggatttt
     2641 gggacggtcg gcgtcggata tgaccgggcc ctcgccggca agatgcatgt cgctgatcgc
     2701 ctcctcggcg gcggccgcat aatcgtagcc gccatatttg atgtcgatgt cgatggcggt
     2761 cgggacgaga tcgctcaaac cctgcgggat aattccaggc ggcggcgcga tcgagccggc
     2821 ggcgaagcgt atttcggtat cgcctttcgc gggaaatttg tcggcgccga aacgcatctt
     2881 gaaatcgccg agcttgaccg gccctttcgg cgtatcgacc tcgatgtttt gcgccgcgat
     2941 gtcctcgccg aatttgtcgg cgagcgggat gagcccgcgc agcttcgctt tcagcgcgtc
     3001 ttcgtcggcg gcgagcgcgg cgcggctcgg atgagcgacc aagaacgccc aaaggtcgag
     3061 agcttcgcgc atgcgcatat tgtcgagcgt cacgttgaag gagaggccgc cggccttgat
     3121 cgagacgggg tccggctggg gtccgtccgc ttttttcgga ttgaccaaca tcgtcatctg
     3181 gccggcttcc tcgcgccaat cgcccgagaa gccgccgcca tttgcggcgg cgccggtccc
     3241 cgtcatgtga gcgggaccga acgtgatcgt ctcgtccgct tccggcgtct ggatgccgac
     3301 attcgccttc tccatcttca cttccgcgct gcggaagccg tacagcgccg gatcgaacag
     3361 gccgtcgaac gtgtagccgt cgacatggac ggagaagccg ccgtccttgg tcttcacggc
     3421 ggctggcgga taggcctcct gcgcgacgcg ccacgcgccg tcgctctgct gaacgagctt
     3481 ggctttcagc ggcgcgatgt cgatgctgac gccctcgctc gccagcggcg cgagaagctt
     3541 ggcgaaatcg accgtgacgg cgtagtgatc gccctcgacg gcgacgctcg ccgcgtcctt
     3601 gccgagatat tgcgcaaaga gagcgctgag cttctgcgcg ccgtcggacg tcggggcgac
     3661 atcgccggcc aaggccgcga gcgggacggc gcagagcgcg gtcgcgccga tcagggcgtg
     3721 gcgcagcttg aagccgcccg cgcgaatgaa gtgccgcatg aaagtctccc cgacggtccg
     3781 ccgcgcggcg ctcacaccgc gggcgggttc aatcctagcc cataacagcg cccaaaccga
     3841 aatagcgcgg gcagacgaat ccatggctgg atccgtccgt ccggcggcaa agatttggag
     3901 tcgttatggc gtccgggcgg atcgtcgccc ggctcacatc gccggcaggg ccgccggact
     3961 cgacgaatgc ggaagctgcg cccgcgcaac ccccgccttg accgcggcct ggaccttttc
     4021 gaaggcccga acctcgatct ggcgcacccg ctcgcgcgag acgccgaatt cagcggagag
     4081 ctcttcgagc gtcaccggct cgtcggcgag ccgccgcgct tcgaatatgc gccgctcgcg
     4141 cggattgagc accgagaggg cgctcttgag cgcatcgagc ctggtgttgc tttcctcgcg
     4201 gtcggcgagc gccgattcct gatcggcctg gtcgtcggcg agccagtcct gccattcgcc
     4261 ctcgccctct tcgcgcaagg gcgcgttgag cgaggcgtcg ccgccgaggc ggcgattcat
     4321 gtcgaccacg tcctgctcgg gcacgccgag gcggtgggcg atttgggcga cctgatcggg
     4381 cttgagatcg ccctcgtcga gcgccgagat gcggctcttg gccttgcgca gattgaagaa
     4441 cagcttcttc tggctggcgg tcgtgcccat tttcaccagc gaccaggaac gcaggatgta
     4501 ttcttgaatt gacgcgcgga tccaccacat agcgtatgtg gccaggcgga agcctttatc
     4561 cggctcgaag cgcttgacgg cctgcatcag gccgacattg ccttcggaaa tgacctcgcc
     4621 gatcggcagg ccatagccgc gatagcccat cgcgatcttg gcgacgaggc gcaggtggga
     4681 ggtgaccagc ttatgggcgg cgtcgcggtc gccgtggtcg cgccaccgct tggcgagcat
     4741 atactcgtcc tgcggctcca gcatggggaa ccgcttgatc tcggagagat agcgcgaaag
     4801 accggattct ccggcgatga caggcagggc ggcagccatg aaacctcctc tcggagctcc
     4861 ccttagaggc gcgctccggc ggccgcgcgt gaggcgcacg ccgcagtgca atataggcgt
     4921 ctgtgaccat ggccgaaaga gggccagggc gctgtggcaa aacgcacaga cgcgacagtc
     4981 gtttcaccgc cgtccggcgc cgccccgctt aattgtcgcg ctcggccagc gccttgataa
     5041 gtctagtaaa atcctccggc ggcgggctct cgaaggataa aatctcgccg gagatcggat
     5101 gcgcgaagcc gagtttggcc gcgtgcaacg cctgcctgtc cagcttggcg agcgcagctt
     5161 gcgccggctc ggacaattgc gccgccttgc ttttgaagcc cgaaccatag accgaatcgc
     5221 ccaagagcgg atggccgata tgggcgagat gcacgcgaag ctggtgcgtg cggccggtct
     5281 ccagccggca ggcgatcagg ctcgccgggc cgagcgcgcg ctgcagccgc caatgagtga
     5341 ccgcgcgccg cccgctttcc gcggcggcga cggccatttt ctcgcgctgg cgcgcatgcc
     5401 gcgcgagcgg cgcgtcgatc gttccggccg gccggcccat cgcgccccag accaaagcga
     5461 gatattcgcg aatcagcgag ccgctgcgcc catgatcggc gaagagctcc gccaagccgc
     5521 gatgggcggc gtcggtcttg gcgacaacga gcaggccgga tgtgttcttg tccagccggt
     5581 gaacgatccc cggtcttttg acgccgccaa tgcccgaaag gctgtcgccg caatgggcga
     5641 tcagggcatg gaccagcgtg cctttggcgt ggccggcggc gggatgcacg accaagccgg
     5701 ccggcttgtc gagaaccaga agatgccggt cctcgaaaac gatatcgagc gcgatgtctt
     5761 cgccttcgag cttcgaaggc tccggcgcgg gaacgcgcag cgcgacgcgg tcgcccgccg
     5821 aaagcttggc cgccggatcg cggagaggcg cgccgttcac cgcgacctcg ccggcttcga
     5881 tcagcgcctt cagccgcgtg cgcgaaaggg cgagatcgga ctcagccgcc gcgcgggcca
     5941 gatagcggtc aagccgttcg ccgggcgcgc cgggcgcgac cgtgaaagcg agcggttcgc
     6001 cggccggaag caagttaccg ccgccgtctt tcgcgctcgc gcggcgcgtg cgccttggcc
     6061 ggcggcgaag cgagcgggcc gagcgcggcg acgatttcgg cttcgctcgg cctaccgccc
     6121 gggacgcgct cgtcgagcgc gcggcgcatg gcggaaagat gatccgccgt cgtgccgcag
     6181 cagccgccga tgatcctggc gcccgaatcg gcggcgaggc gggcataatc cgccatcaaa
     6241 gccggcgtgc cggaataatg gatgtgggcg ccgcgccatt gcggaatgcc ggcattggct
     6301 ttggcgatga cgatcgcgtc cggccgcttt tccgtcatcg ccagaatcga gacgagaaga
     6361 tcggacgcgc cgacgccgca attggcgccg aacgccaaag gagacggcgc gaagctctcg
     6421 gtcaactcgg ccaaggcctc aggcgcgagg cccatcattg tcttgccggc cgtgtcgaaa
     6481 ctggccgtca ccgtataggg catgccgagc gcgcccgccg ccttggccgc cgcgcgcatc
     6541 tcctcggggg ccgacatcgt ctcgatccaa acgaggtcgg cgccgcccgc tttcagaccc
     6601 tcgatctgct cggcgaagac ggcgaccgcc tcctcctcgg aaagcggccc gagcggcgcc
     6661 agcaagtcgc cggtcggtcc gaccgagccg gcgattgcga ccggccggcc ggcgcgatcg
     6721 gccgtttcgc gcgcgatctc agccgcacgg cgattgagct cgccgacccg cccctcgagg
     6781 ccatgcagca tcagacggcg ccgattggcg ccgaaactgt tggtcaggat gatgtcggcg
     6841 ccggcttcga cgaaggcgcg gtgaagggct aagaccttgg cgcgctggct gtcgttccac
     6901 gattccgggc tttcgccgga ggaaaggccc atcgcgaaga gattggtgcc cgtcgcgccg
     6961 tcggcgagca ggggacggcc ggtcgcgagc agagattcga acatggcgtc ccttggcggt
     7021 cgagtaaaat tggaaggaat gcgcccgcct tcggcgcggc aagagccgca cacttaagga
     7081 gcggcccgcc tcgacgcaag cgataggccc gatcggcccg tccgcaagcg tacggaattg
     7141 cgtttcgcga catttttgtg acccggtcgc ctccgctccg caaaatcaga cttggcgctc
     7201 gccttgattc cggcgccgcg atggactatc cgggacgcca tcgatttcgg ccgaagactt
     7261 ggcctcgcat cgaacgatgc gtgctgacga tccccttaca tcgatgatcg accggcgctt
     7321 tgcgcctatg cagtactatt ggacttgaag gaaggcttcc catggctacg ggaactgtga
     7381 aatggtttaa cgcgacgaag ggctatggat tcattcagcc cgacgacggc ggcaaagatg
     7441 tttttgtcca tgtcagcgcc gtcgagcgcg ccggcatgcg cgatctggtc gaaggtcaga
     7501 aagtcgctta cgaggtcgtc gccgacaagc ggacgggcaa atcctcggcg ggcaatttgc
     7561 gcgctggctg aggcgccgtt aaagatcggt cgcgacgcca agcgtcgcgg ttaaggccaa
     7621 ggcctcggcg gcgacgccga ggcctttcgt ttgggccttc aggcggattg cagcgaggag
     7681 cgggcgccaa tggcgggagg aacctttctc ggtctcgata tcggcacgtc ctcggtcaaa
     7741 gccgtcttga tcgacgaaaa tcagcatctc atcgcggaaa gcgcgccggc gctgagcgtg
     7801 tcgcggccgc atcctttgtg gagcgagcag aacccgcacg attggaccca agcggtcgag
     7861 gacgcggtcg ccgcgattcg ccgccaagcc ggcgcggcct tttcgcgcct ggccggaatc
     7921 ggcctttccg gccagatgca tggcgccgtc ctgctgggca aggacgatcg cgtgctgcgc
     7981 ccggcgatcc tctggaatga tggacgctct ttcgccgaat gcgtggagtt ggagcgccgc
     8041 gcgcccgatt cgcgccagcg cgccggcaac atcgcgatgg ccggcttcac cgcgccgaaa
     8101 ctgctttggg tcgccaagca cgagccggac gttttcgcgg cgacggagcg cgtgctgttg
     8161 cccaaggatt acgtgcggct cttcttgagc ggcgaatcgg tctcggaaat gtccgacgcc
     8221 tccggcacgc tctggctcga cgtcgccaag cgccgctggg actcagagct cctcgccgcc
     8281 tgcggcttga gcgagaaggc gatgccgcgc ctcgttgaag gctctgaagt ctcgacctct
     8341 ctcgcgccgg ggacggcgag ccgctggggc cttgccgggc ggcgcatacc gatcgccggc
     8401 ggcgccggcg acaacgccgc ggcggcggtc ggcatcggcg cgaccggcgc cggccaaggg
     8461 ctcgtctcgc tcgg
//
LOCUS       DATGJW010000240         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_875737, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000240 DATGJW010000000
VERSION     DATGJW010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_875737"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1078
                     /locus_tag="VKU03_07450"
     CDS             <1..1078
                     /locus_tag="VKU03_07450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010975715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71136.1"
                     /translation="SRRAFLRFKRNRAALGGLIALVVIALFCALGPALSPHAYDQVYR
                     DYVKTPPSFAAHPTNKEQATILERIAERLGADVVAAAPSASDARATLQAPKAIDETLL
                     LDAFTRSNVFADAHIVATEDEGRRLILETRVRRETFLLGADSNGRDLLTRLMVGGRVS
                     LLVGALASSVALVIGVAYGSIAGYSGGRLDALMMRIVDILYALPFVFFVILLVVVFGR
                     HFVLVFVAIGAVEWLDMARIVRGQTLSLKRQDYVLAAEALGARAGGVLIRHIVPNLVG
                     PVIAYLTLVAARVILLESFLSFLGLGVQEPMTSLGALIADGARNIEDAPGMLLFPAAL
                     LSAILLALSVVGEGVRDALDVRGH"
     gene            1081..>2368
                     /locus_tag="VKU03_07455"
     CDS             1081..>2368
                     /locus_tag="VKU03_07455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ71137.1"
                     /translation="MAPLLAISDLHIRFRNDGRDVEAARGIDLHIERGETVALVGESG
                     SGKTQTMMAAIGMLPANGSATGSIRFEGDEILGLPDQALDRLRGSRITMIFQEPMTAL
                     DPLFSIGAQIMTPLRVHGRLSKQAARRRALELLALVGLDPPERRFKAAPHQLSGGQRQ
                     RAMIAMAIANNPALLIADEPTTALDVTVQAQILDLLRDLQHRLGMAILFITHDFAILK
                     RFVDRVYVMRSGEVVETGLIEEVMARPRAATTKALIEATPPDRKTPPRVDAPIILEAR
                     EITVDYTQPSSLFRRGSVFRAVDKASLDLRRGQTLGIVGESGSGKSTLARALLRLVPY
                     QGSIRFEGRDLAALEAASLRAQRRQMQLVLQDPFGSLSPRLTIGAIVSEGLLIHEPGM
                     SAAERKRGAADELEAVGLSRRFLDAFPHELSGGQRQR"
BASE COUNT          366 a          782 c          737 g          483 t
ORIGIN      
        1 aagccgccgc gccttcctgc ggttcaaacg caatcgcgcc gccctcggcg ggcttatcgc
       61 gctcgtcgtc atcgcgcttt tctgcgcgct tggtcctgcg ctgtcgccgc acgcctatga
      121 ccaggtctat cgcgactatg tcaaaacgcc gccgtctttc gcggcccatc cgacgaacaa
      181 agagcaagcg acaatcctcg aacgcatcgc cgagcggctg ggcgccgatg tcgtcgcggc
      241 ggcgccgtcg gccagcgacg cgcgcgcgac tttgcaagcg ccgaaggcga tcgacgagac
      301 attgcttctc gacgctttca cccggtccaa tgttttcgcc gacgcgcata tcgtggccac
      361 agaagacgaa gggcgtcgcc tgattttgga aacgcgcgta cgacgcgaga catttcttct
      421 cggcgccgat agcaatggcc gcgacttgct cacgcgcctg atggtcggcg gccgcgtctc
      481 cctgctcgtc ggcgcgctgg cgtcatctgt cgcgctcgtc atcggcgtcg cctatgggtc
      541 gatcgccggc tattccggcg gccgcctcga cgctttgatg atgcgcatcg tcgatattct
      601 ttacgccctg ccttttgttt ttttcgtcat tttgctggtc gtcgtcttcg gacggcattt
      661 cgtcctggtc ttcgtggcga tcggcgcggt ggaatggctg gacatggccc gcatcgtccg
      721 cggccagacg ctgagtttga agcggcagga ctacgtacta gcggcggaag cgctcggcgc
      781 gcgcgccggc ggcgtactca tacgccatat cgtccccaat cttgtcggtc cggtcatcgc
      841 ctatctgact ctcgtcgccg ccagggtcat cctgctcgag agctttcttt cctttctcgg
      901 cctcggcgtg caggagccga tgaccagcct cggcgcgctc attgccgatg gcgcgcgcaa
      961 tattgaagac gcgccaggca tgctgctctt tccggcggcg ctgctctcgg cgattctcct
     1021 cgccttgagt gtcgtcggcg aaggcgtgcg cgacgctctc gatgtgcggg ggcattgaga
     1081 atggcgccgc tcctcgccat cagcgatttg cacatccgtt tccgcaacga cgggcgcgac
     1141 gtcgaggccg cacgcgggat cgatcttcat atcgagcgcg gtgagacggt cgcgctggtc
     1201 ggcgagagtg gctcgggcaa aacccagacg atgatggccg cgatcggcat gcttccggcc
     1261 aacgggagcg cgaccggctc gatccgtttt gagggcgatg aaatcctcgg ccttcccgat
     1321 caggcgctcg atcgcctgcg cggctcgcgc atcaccatga tctttcagga gccgatgacc
     1381 gcgcttgatc cgcttttttc gatcggcgcg cagatcatga cgccgctcag ggtgcacggt
     1441 cggttgtcga aacaggcggc gcgccggcgc gcgctggaat tgctcgccct tgtcggcctc
     1501 gatccgccgg aacggcgctt caaagccgcg ccgcatcaat tgtccggcgg ccagcgccag
     1561 cgcgccatga tcgccatggc gatcgccaat aatccggcct tgctgatcgc cgacgagccg
     1621 acgaccgcgc tcgacgtgac ggtacaagcg cagattctcg atctgctccg cgatcttcag
     1681 catcgcctcg gcatggcgat ccttttcatc acccatgatt tcgcgatcct caaacgtttt
     1741 gtcgatcgcg tctacgtcat gcgttccggc gaagtcgtgg agacgggcct gatcgaagag
     1801 gtcatggcgc gcccgcgggc tgcgacgacg aaggctctga tcgaggcgac gccgcctgac
     1861 cgcaaaacac cgccgcgagt cgacgcgccc atcattctcg aagcgcgaga gatcacggtc
     1921 gattatacgc agccctcgag cctgtttcgc cgcggaagcg tcttccgcgc ggtcgataag
     1981 gcgagcctcg atctcaggcg tggtcaaacg cttggaatcg tcggcgagtc cgggtcaggc
     2041 aaatcgacgc tcgcccgcgc cttgcttcgt cttgttccgt atcaaggctc tattcgcttt
     2101 gaagggcgcg atctcgccgc gctcgaagcg gcctccctcc gcgcccagcg gcgacagatg
     2161 cagctcgtct tacaggatcc ctttggctcg ctgtcgccgc gcctgacgat cggcgcgatc
     2221 gtttccgaag gtttgctgat tcacgaaccg gggatgagcg cggcggagcg aaagcgcggc
     2281 gccgcggacg aattggaggc ggtgggacta agtcggcgtt ttctcgacgc ctttccgcat
     2341 gaactctccg gcggtcagcg ccagcgcg
//
LOCUS       DATGJW010000241         5112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_876190, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000241 DATGJW010000000
VERSION     DATGJW010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5112
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_876190"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1857
                     /locus_tag="VKU03_07460"
     CDS             <1..1857
                     /locus_tag="VKU03_07460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein meaA"
                     /protein_id="HLJ71138.1"
                     /translation="TGYDSDHPLAKGEVGKVGVAICHLGDMRALFENIPLAEMNTSMT
                     INATALWLMALYIAVAEEQGAARASLQGTTQNDIVKEYLSRGAYIFPPAPSLRLTKDL
                     ILFSARETPKWNPMNVCSYHLQEAGATPAEELSYALAAALAVLDMVRASGEVDEKGFA
                     DVVGGMSFFVNAGMRFLTELAKMRAFVELWDEICQERYAIADPAKRRFRYGVQVNSLG
                     LTEQQPENNAYRILIEMLAVVLSRNARARAVQLPAWNEALGLPRPFDQQWSLRLQQIM
                     AYETDLLEFGDIFDGSREIAAKVAALKAEARAELAAIEAMGGAIAAVESGYMKRRLVE
                     SNTRRVEAIERGEQIVVGVNRFVESEPSPLIAGEGTILAIPPHAEAEQIARLSAWRAS
                     RDQAAVAAALHELSGAAKDGQNLMAPSIAAAKAGVTTGEWSAALRAAFGEYRAPTGVS
                     STARQQAADLDEIRAEVERVSLKLGRRLKLLIGKPGLDGHSNGAEQIAVRATDAGMDV
                     VYDGIRLTPQEIVERAKEGVHCIGLSILSGSHLEMAGEVLGGLRAAGLGHLPVIVGGV
                     IPPADADKLKAQGAAAVYTPKDFAINRLVGDIVRLVEPQAKQAAESGLVTRN"
     gene            2032..3501
                     /locus_tag="VKU03_07465"
     CDS             2032..3501
                     /locus_tag="VKU03_07465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006334834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family MFS transporter"
                     /protein_id="HLJ71139.1"
                     /translation="MSKPRGLSDGQKRRIMVGVMMAMMLAALDQTIVAPALPIMGASL
                     GDSEFLSWIISAYLVTGTAVTPLYGKFSDIHGRRPALFLSLGVFLIGSVVCALAPSMA
                     AIIVGRAVQGLGGGGLLALSQTVVADIASPRERARYVIYFSLVWATSSVAGPIIGGFF
                     AQHLHWSLIFWINLPIGALAFAMTNRVLKTLPQIKRDHRLDLVGAALIVGATGAFLLL
                     LTLGGVRYPWTSPPILALALLAALLIAGFLLDMARAKEPLIPLSIFRNEVVGSASLSM
                     FFTMFAFVGVTVYLPIYFEFALGMAPTASGAGLIVLLAGSVAGANVTGRSMPRVVHYK
                     RMAMTGLACAIIASAALSLLAEHLAFWQVEALMLALGVGVGAVFPTLTVSVQNAIDPR
                     DLGIGTATLAFLRSLGGAIGVAALGAVVLAYGIVSDSGLSHAGGAFDPEIARRAGAAF
                     VDVFALQGAALLASLIFLAIMEERPLRGPAAHKATTTEV"
     gene            complement(3588..4313)
                     /locus_tag="VKU03_07470"
     CDS             complement(3588..4313)
                     /locus_tag="VKU03_07470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP reductase"
                     /protein_id="HLJ71140.1"
                     /translation="MARVAVVTGGTRGIGAAISKALHAAGCKVAATYQGNDEAAAKFK
                     AETDVQVYKWDVASYEACVAGLDQVVKDLGPVDILVNNAGITRDAMLHKMKPEQWYAV
                     VNTNLNSLFNMCRPVIEGMRERGFGRIVNISSINGQKGQMGQTNYSAAKAGDIGFTKA
                     LAQENAAKGVTVNAICPGYIATEMVLAVPKDVLEKSILPTIPVRRLGQPEEVARCVVF
                     LASDEAGFITGATLTANGGQYMT"
     gene            complement(4483..>5112)
                     /locus_tag="VKU03_07475"
     CDS             complement(4483..>5112)
                     /locus_tag="VKU03_07475"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892545.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HLJ71141.1"
                     /translation="QNKAEAAQKAGRFKDEITPVAVPSKKGDIVVDQDEFIRPGVVYD
                     NLAKLKPAFSKEGTVTAGNASGINDGAAALVLMSAAEAKKRGLAPLARIVSWATAGVD
                     PSIMGAGPIPASRKALEKAGWRVKDLELVEANEAFAAQALAVNKDMGWDPSIVNVNGG
                     AIAIGHPIGASGARILVTLLHEMGRRNAKKGLATLCIGGGMGIAMAVER"
BASE COUNT          768 a         1691 c         1663 g          990 t
ORIGIN      
        1 accggctatg acagcgacca tccgctcgcc aagggcgagg tcggcaaggt cggcgtcgcc
       61 atctgtcatc tcggcgacat gcgggctttg ttcgagaaca ttccgctcgc cgagatgaac
      121 acgtcgatga cgatcaacgc cacggcgctt tggctgatgg cgctctatat cgccgtcgcc
      181 gaggagcagg gcgcggcgcg ggcgagcctg caagggacga ctcagaacga tatcgtcaag
      241 gaatatctgt cgcgcggcgc ctacatcttt ccgcccgcgc cgtcgctgcg cctgaccaag
      301 gatttgattc tgttctcggc gcgcgagacg ccgaaatgga atccgatgaa cgtctgctcg
      361 taccatttgc aggaagccgg cgcgacgccg gccgaggagc tgagctatgc gctggcggcg
      421 gcgctggcgg ttctcgacat ggtgcgcgct tccggagagg tcgacgaaaa aggcttcgcg
      481 gacgtggtcg gcggcatgtc cttcttcgtc aatgccggca tgcgcttcct caccgaactg
      541 gcgaaaatgc gcgccttcgt cgagctgtgg gacgaaatct gccaggagcg ctacgccatc
      601 gccgatccgg ccaagcgccg ctttcgctac ggcgtgcagg tcaattcgct cggcctcacc
      661 gagcagcagc cggagaacaa cgcctaccgc attctcatcg aaatgctggc ggtcgtgctg
      721 tcgcgcaacg cgcgcgctcg ggcggtgcaa ttgccggcgt ggaacgaggc gctcggcctg
      781 ccgcggcctt tcgatcagca atggtcgctc aggcttcagc agatcatggc ctacgaaacc
      841 gatttgctcg aattcggcga tattttcgac ggcagccgcg agatcgccgc caaggtcgcc
      901 gcgctgaagg cggaggcgcg cgccgagctc gcggcgatcg aggcgatggg tggggcgatc
      961 gcggcggtcg aaagcggcta tatgaagcgc cgcctcgtcg aatcgaatac gcgccgggtc
     1021 gaggcgatcg agcgcggcga gcagatcgtc gtcggcgtca accgcttcgt cgaaagcgag
     1081 ccctcgccct tgatcgcggg ggaggggacg atcctcgcca ttccgccgca tgccgaggcc
     1141 gagcagatcg cccggctcag cgcttggcgg gcgagccgcg accaagcggc ggtcgccgcc
     1201 gcgctgcacg aactgagcgg cgccgccaaa gacgggcaga acctcatggc gccctcgatc
     1261 gccgccgcca aagccggcgt cacgacgggc gaatggagcg cggccttgcg cgccgccttc
     1321 ggcgaatacc gggcgccgac cggcgtttcg agcacggcgc gccagcaagc ggccgatctc
     1381 gacgaaattc gcgccgaagt cgagcgcgtc tcgctgaagc tcgggcggcg gctgaagctg
     1441 ctcatcggca agcccggcct tgacggccat tccaacggcg ccgagcagat cgcggtgcgc
     1501 gccaccgacg ccggcatgga cgtcgtctat gacggcatcc gcctgacgcc gcaggaaatc
     1561 gtcgagcgcg ccaaggaagg cgtccattgc atcggcctgt cgatcctttc cggctcgcat
     1621 ctggaaatgg cgggcgaagt gctcggcggc ttgcgggcgg cggggctcgg ccatctgccg
     1681 gtgatcgtcg gcggcgtcat tccgccggcc gacgccgaca agctcaaggc gcaaggcgcg
     1741 gccgcggtct atacgccgaa ggatttcgcc atcaaccgcc tggtcggcga catcgtcaga
     1801 ttggtcgagc cgcaggcgaa acaagccgcg gaaagcggcc tcgtaacgcg aaattgagcg
     1861 gcgcgcggcg ggcggcttct tgtcgagcgc cgccgcaagt gtcattcccg cgcaaaagcg
     1921 ggaagccgat acggcctctt ctaccgcttc gctttttttg cgcgccagcc attcaggcgc
     1981 ggaatccctc ttttctcgct ctattctttg aggtcttttc catcgatgga tatgtcgaag
     2041 ccaagaggtt tatcggacgg gcagaagcgc cgcatcatgg tcggcgtgat gatggcgatg
     2101 atgctggcgg cgctcgacca aacgatcgtc gcgccggctt tgccgatcat gggagcctcg
     2161 ctcggcgatt cggaattcct gtcctggatc atctcggctt atctcgtcac cggcaccgcg
     2221 gtgaccccgc tttacggcaa attttctgac attcatggtc ggcggccggc gctttttttg
     2281 tcgctcggcg tctttctgat cggctccgtc gtctgcgcgc tggcgccgag catggccgcg
     2341 atcattgtcg gccgcgccgt gcagggcctg ggcggcggcg gcttgctcgc tctatcgcag
     2401 acggtcgtcg ccgacatcgc ctcgccgcgc gagcgggcgc gctatgtgat ctatttttcc
     2461 ctggtctggg cgacatcgag cgtcgccggc ccgatcatcg gcggcttctt cgcccagcat
     2521 cttcactggt cgctgatttt ctggatcaac ctgccgatcg gcgccctggc tttcgcgatg
     2581 accaatcgcg tcttgaagac gctgccgcag atcaagcgcg atcatcgtct cgacctcgtg
     2641 ggcgccgcgc taatcgtcgg cgcgaccggc gcctttctgc tgctgctcac actgggcggg
     2701 gtgcgctatc cctggacctc gccgccgatt ctggccctcg ccctgctcgc cgcgctgctg
     2761 atcgccggct tccttctcga tatggcccgc gccaaggagc cgctgatccc gctcagcatt
     2821 ttccgcaacg aagtggtcgg ctcggcctcg ctgtcgatgt tcttcacgat gttcgccttt
     2881 gtcggcgtta cggtctatct gccgatctat ttcgaattcg cgcttggcat ggcgccgacc
     2941 gcctccggcg cgggcctcat cgttcttctc gccggcagcg tcgccggggc caatgtcacc
     3001 gggcgctcga tgccgcgcgt cgttcactat aagcgcatgg cgatgaccgg gctggcctgc
     3061 gcgatcatcg cctcggccgc cctgtcgctg ctcgccgagc atttggcttt ctggcaggtc
     3121 gaggccttga tgctggcgct cggcgtcggc gtcggcgcgg tttttccgac gctcaccgta
     3181 tcggtgcaga acgccatcga cccgcgcgat ctcggcatcg gcaccgcgac gctcgctttc
     3241 ctgcgctcgc tcggcggcgc gatcggcgtc gccgctctcg gcgcggtcgt cctcgcctat
     3301 gggatcgtca gcgattccgg cctttcgcat gccggcggcg ccttcgatcc agagatcgcg
     3361 cgccgcgccg gcgccgcttt tgtcgatgtc ttcgcgctgc aaggcgcggc attgctggcg
     3421 agtttgatct tcctcgccat catggaggag cggccgctca ggggaccggc ggcccacaag
     3481 gcgacgacca cggaagtatg atccgtcatc gcgagcgaag ccgcctagtc gtagggcgac
     3541 gttccgagag cggatcgctt cgctgcccgc aatgaaggcg gagcggctta tgtcatgtac
     3601 tgaccgccat tcgcggtcag cgtcgcgccg gtgatgaagc cggcctcgtc cgaagcgaga
     3661 aacacgacgc agcgcgcgac ctcttcgggt tggccgaggc gcctgacggg aatggtcggc
     3721 aggatcgatt tctcgagcac gtccttcggc acggccagca ccatttcggt ggcgatatag
     3781 ccggggcaga tggcgttcac ggtgacgcct ttggcggcgt tttcctgggc gagcgccttg
     3841 gtgaagccga tatcgccggc cttggccgcc gaataattgg tctggcccat ctggcccttc
     3901 tggccgttga tcgaggaaat gttgacgatg cggccgaagc cgcgctcgcg catgccctcg
     3961 atcaccggcc ggcacatatt gaacagcgaa ttgaggttgg tgttgaccac cgcgtaccat
     4021 tgctcaggct tcatcttgtg cagcatggcg tcgcgggtga tgccggcatt gttcaccaga
     4081 atgtcgaccg ggccgagatc cttcaccacc tgatcgagcc cggcgacgca ggcctcgtag
     4141 ctggcgacgt cccatttata gacctgaacg tcggtctcgg ctttgaattt cgccgcggct
     4201 tcgtcattgc cttgataggt ggcggcgact ttgcaccccg ccgcatgcaa cgccttggat
     4261 atggccgcgc cgatgccgcg cgtgccgccg gtgacaactg caaccctggc cattggcgtt
     4321 ccctccggtt cgtttctccc tctcctcctc cgctttcgcg ggggaggggg accgcgcgtc
     4381 gcgcggtgga gggggtacgg cggttcatga caatctcaga ccccgcggcc ccctccacca
     4441 tgcttcgcat ggttctcctc ccccgtcttc gatgggggag gattatcgct cgaccgccat
     4501 cgcgatgccc atgccgccgc cgatgcacaa cgtcgccagg cccttcttgg cgttgcgccg
     4561 gcccatttca tgcagcagcg tcaccagaat gcgcgcgccc gaggcgccga tcggatggcc
     4621 gatggcgatc gcgccgccat tgacgttcac gatcgaagga tcccagccca tgtccttgtt
     4681 caccgccagg gcttgcgcgg cgaatgcttc gttggcttcg acgagttcga ggtccttgac
     4741 cctccacccc gccttttcga gcgccttgcg cgaggcgggg atcgggccgg cgcccatgat
     4801 cgatggatcg acgccggccg tcgcccagga gacgatgcgg gcgagcggcg ccaagccgcg
     4861 cttcttggcc tcggcggcgc tcatcagcac gagggccgcc gcgccgtcat tgatgccgga
     4921 agcgttgccc gcggtgacgg tgccttcctt ggagaaggcc ggcttcagct tggcgagatt
     4981 gtcgtagacg acgcctggac gaatgaactc gtcctggtcg acgacgatgt cgcctttctt
     5041 ggacggcacg gcgaccggcg tgatctcgtc cttgaagcga cccgctttct gcgcggcttc
     5101 ggccttgttc tg
//
LOCUS       DATGJW010000242         2352 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1496544, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000242 DATGJW010000000
VERSION     DATGJW010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2352)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2352)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2352
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1496544"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(57..656)
                     /gene="plsY"
                     /locus_tag="VKU03_07480"
     CDS             complement(57..656)
                     /gene="plsY"
                     /locus_tag="VKU03_07480"
                     /EC_number="2.3.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008374 - O-acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006654 - phosphatidic acid biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate 1-O-acyltransferase PlsY"
                     /protein_id="HLJ71142.1"
                     /translation="MVGVSYGGALALALGYCLGSIPFGLVLTRLIGAGDLRAIGSGNI
                     GATNVLRTGRKGLAAATLALDALKATLAVVVAWAAFGPPTALAAAAGAVLGHMYPVWL
                     KFRGGKGVATYLGGLIGLAWPAAIVFAIVWLAVAALTRYSSAAALAATIASPVALAAR
                     GEGGAALAFAALSALIWFKHSANIARLLKGSESKIGRKT"
     gene            complement(711..2003)
                     /gene="pyrC"
                     /locus_tag="VKU03_07485"
     CDS             complement(711..2003)
                     /gene="pyrC"
                     /locus_tag="VKU03_07485"
                     /EC_number="3.5.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004151 - dihydroorotase activity
                     [Evidence IEA];
                     GO_process: GO:0009220 - pyrimidine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroorotase"
                     /protein_id="HLJ71143.1"
                     /translation="MPSAPPIAFLNARLIDPVQETESRGGLVVIEGVIAGVGSKVTRD
                     QLPAEARIVDCEGDVVSPGLVDARAFVGEPGAEHRETIASLSAAAAAGGVTTVVTRPD
                     TNPPVDDPAMVDFILRRARDDAKVRIHPAAALTRGLKGEEIAEIGLLAEAGAIAFCDG
                     ARSIADALTMRRALTYARDFDALVAPVADDRSLSGGGVMGEGLRASWLGLSGVPREAE
                     TIVLERDLRLVALTAARYHAALISNRLSLEAMARAREAGLPATCGVSINHLTLNELDV
                     GDYRTFLKLNPPLRAEDERMALVEALGAGLIDVVCSDHDPQDVETKRLPFAEAAAGAI
                     GVETMLSASLRLVAGGHVALPRLIRAMTARPAEILRLPVGRLDVGAPADIIRFDPDEP
                     YALDPASLHSRCRNTPFDGARLEGRVKMTLVAGWIAHE"
     gene            2200..2276
                     /locus_tag="VKU03_07490"
     tRNA            2200..2276
                     /locus_tag="VKU03_07490"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:2234..2236,aa:Arg,seq:acg)
BASE COUNT          405 a          809 c          811 g          327 t
ORIGIN      
        1 ccaatcaaaa cgctgctggt cggtgaggcg caactccggt ttgatcgcca ttcctttcag
       61 gttttccgcc cgattttcga ctcggagcct ttgagaaggc gggcgatgtt cgcgctgtgc
      121 ttgaaccaga tcagcgccga cagcgccgcg aacgccaaag ccgcgccgcc ctcccctctc
      181 gccgccaggg cgaccggcga agcgatggtc gccgcgagcg ccgccgccga cgagtaacgc
      241 gtgagcgccg caaccgcgag ccagacgata gcgaacacga tcgccgccgg ccaggcaagg
      301 ccgatgaggc cgccaaggta ggtcgcaacg cccttgccgc cgcggaactt gagccagacc
      361 ggatacatgt ggccaaggac cgcgcccgcc gcggccgcca gcgccgtcgg cggtccgaac
      421 gccgcccacg cgaccacgac ggccagcgtc gccttcaagg cgtcgagcgc gagcgtcgcc
      481 gccgcaagcc ccttgcggcc ggtgcgcaag acattggtcg cgccgatgtt cccggagccg
      541 atcgcgcgca aatcgccggc cccgatgagc cgggtcagca cgagcccgaa cggaatcgat
      601 cccagacaat agccgagcgc cagcgccagc gccccgccgt aagaaacccc gaccatgcgc
      661 gcttccagcc gacgaaggtt gaacgaaagt gcttttcgtt ttcgatgaga ctattcgtgc
      721 gcgatccacc cggcaaccag cgtcatcttg acccggccct cgagccgggc gccgtcgaag
      781 ggcgtgttgc ggcagcgcga atggagcgag gccggatcga gcgcgtaagg ctcgtcggga
      841 tcgaagcgga tgatgtcggc cggggcgccg acgtcaagcc ggccgaccgg cagccgcagg
      901 atctcggccg ggcgcgcggt catcgcgcga atgaggcggg gcagcgccac atgtccaccg
      961 gcaaccaggc gcaggctcgc ggagagcatc gtctcgacgc cgatcgcgcc ggccgctgct
     1021 tccgcgaacg gcagccgctt ggtttcgacg tcctgcgggt cgtggtcgga acaaacgacg
     1081 tcgatgaggc cggcgcccag cgcctcgacc aaggccatgc gctcatcctc ggcgcgcaat
     1141 ggtgggttca gtttgaggaa ggtgcgatag tcgccgacat cgagttcgtt cagggtcagg
     1201 tgattgatcg agacgccaca ggtcgcgggc aacccagcct ctcgcgcgcg cgccatcgcc
     1261 tcgagcgaca gacggttcga gatgagcgcc gcgtgatagc gggcggcggt cagcgcgacc
     1321 aggcgcaggt cgcgctcgag cacgatcgtt tccgcctcgc gcggcacgcc cgatagcccg
     1381 agccagctcg cgcgcaatcc ctcccccatc acgccgccgc cgctcagcga ccggtcgtcc
     1441 gcgaccggcg cgacgagggc gtcgaagtcg cgggcgtaag tcagggcgcg gcgcatggtg
     1501 agcgcgtcgg cgatcgaacg ggccccatcg cagaaggcga tcgcgcccgc ttcggcgagc
     1561 aggccgatct cggcgatctc ctcgcccttg agccccctgg tcagggccgc tgcgggatgg
     1621 atgcggacct tggcgtcgtc gcgcgcgcgg cgcaggatga agtcgaccat tgccgggtcg
     1681 tcgaccggcg ggttggtgtc ggggcgcgtc acaaccgtgg tcacgccccc ggcggccgcg
     1741 gcggcgctca ggctggcgat ggtctcgcga tgctcggcgc cgggctcgcc gacaaatgcg
     1801 cgcgcgtcga cgaggcccgg cgaaacgacg tcgccctcgc aatcgacgat ccgcgcctcc
     1861 gccggcaatt gatcgcgcgt gaccttggag ccaactccgg cgatgacgcc ttcgatgacg
     1921 acgagcccgc cgcggctctc ggtctcctga acgggatcga tgagacgcgc gttcaggaag
     1981 gcgatcggcg gagcggacgg catgggcggt cgttgcctag cggccggcgc ctgggcgcgc
     2041 aagagcgccc gatcgccaag ggaggatcgc caatggagga aggagcgccg agcggccccg
     2101 acctctaggg tcatgacttc gcgcttgcct gcccaggcaa aagcggctag agcccgccgt
     2161 cgccgctgga gcgcgcttcg ccgctcctca agcggccttg cgcccgtagc tcagctggat
     2221 agagcaccag actacgaatc tgggggtcag gagttcgaat ctcttcgggc gcgccagcaa
     2281 aatcaatagc ttctcacaat ggcaaggcag gtcgcttccc agaaaatgga tttgggaagc
     2341 acatgggaag ca
//
LOCUS       DATGJW010000243        10134 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_358084, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000243 DATGJW010000000
VERSION     DATGJW010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10134)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10134)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10134
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_358084"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1367)
                     /locus_tag="VKU03_07495"
     CDS             complement(<1..1367)
                     /locus_tag="VKU03_07495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004121864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ71144.1"
                     /translation="MSACAGCGFEIQSGFAFCPQCGARQPAQCAACGYALAPDFAFCP
                     KCGAATAAAKSPGAAPKAGERSPPASVAAPAAPRRAEPPNRFAEAAPVKMDAHADRRT
                     VTVLFADLSGFTTLSERLDPELVQALQNDIFEEMTAALHRFGGFPDKFIGDALLALFG
                     APTAHEDDPERALHAALDMAERIARLGENWRARLGSPLALHIGVNTGPVVAGGIGAGS
                     AKSYSVTGDTVNTAQRLQSTAARGEVLVGPLTYRLTRHAFAYEALGEASLRGKTGSVL
                     VHRLIGPLEKPRAARGLETIGLHAPLIGRDAELAHMIGSLDLACGGAAQVVRLIGEAG
                     IGKTRLVNEFLARLRDEERFSGIAVRIAVCSPLGEQSFGALAAVLRSAHQIDRNAPAA
                     EAKARLAASLAELGLGADEIRELSPLYCHVLDIGDPDAALSQIEPEQLRRQIFFAIRT
                     VFERWL"
     gene            complement(1373..4909)
                     /locus_tag="VKU03_07500"
     CDS             complement(1373..4909)
                     /locus_tag="VKU03_07500"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing hybrid sensor histidine
                     kinase/response regulator"
                     /protein_id="HLJ71145.1"
                     /translation="MLDGVTVFATAIAYVGALFLIAYWGDRSGRRFMAGRARGAVYAL
                     SLAVYCTTWTYYGSVGIASAHGLDFLPIYIGPVLVVGLGHRLVARIAALAKGQNLTSV
                     ADFVAARYGKAQHVAALVAFVAMIASAPYIALQLKAIAQTILMMVGSLDQGRLAPAAP
                     SPIFFLAVALVLAAFAMAFGTRRIEATEHQDGLILAVAAESLVKLVAFLGVGSFVVWG
                     LFGGLGRLAHIAADNAAIAAIMRTPPDPLNWIVTTLLSAVAILLLPRQFHVMIVENRD
                     ERDIPAAAWRFTLYLAAINLFVVPLAIAGLATFSAGTIDRDYTVLALPLAAGAHGVAL
                     LTMIGGLSAATAMVVVESVALSISVSNNLIMPLILRGRARAAAQEGAIGSLVLFVRRA
                     AILGVLFLGYAYIRIANDAALSSIGLLSFAGIAQIAPAFLGGLFWRRANARGASAGLI
                     IGAAIWFYTLLLPSLDLNSSAFVNFLEQGPLSIAWLKPSALLAITGRPLVSGAVLSLG
                     ANLIAFVLFSLTREANAIERLQAGAFVGAEPAAKAPSLRLWRASTTAGELEATVSLYL
                     GADRARHALESFMGGRGLKFEPAGEADAPLIRHAEHLLASAIGASSSRLVMSLLFRRR
                     AMSSKAALRLIDDASAAIQSNRDLLQHALDHARQAIAVFDRNLALTCWNRAFGELFDL
                     PNPLLRLGVGLDEIVRFNAARGVYGPGASDEFVASRIESLLNDQAPSRLRLYPKGLVA
                     EVRSARLPDGGVVTTYTDVTEAVHAEERLAAVNEQLEQRVRERTEQLERLNQELARAK
                     NAAEDANLSKTRFLAAASHDILQPLNAARLYATSLAEIAGRAKESEPAELARNVDASL
                     EAVEDILGALLDISRLDAGATRPQVAAFPIADPLRQLEIEFAPIAEAKGLKLTFVPSS
                     LIVRSDRLMLRRLLQNLVSNAIKYTPQGRVLVGCRRRGGLCAIEVWDTGLGIPAASQK
                     IVFEEFQRLDRGARAAPGLGLGLSIVERLARVLGHDIQLRSKPGAGSVFSVAAPLATF
                     AKALPRHEPRSREAARADEPLTDLVVLAIDNEPRVLGGLTALLEKWGCRVIAAASLEE
                     SFSALKREGAAPDAIVADYHLDEGDGLAAIAELRAAYGGETPAILATADRDPEMRAAA
                     SRADVAILNKPLRPAALRALLMRCKTMRLAAE"
     gene            5220..5849
                     /locus_tag="VKU03_07505"
     CDS             5220..5849
                     /locus_tag="VKU03_07505"
                     /inference="COORDINATES: protein motif:HMM:NF005754.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="short chain dehydrogenase"
                     /protein_id="HLJ71146.1"
                     /translation="MAEKRLRILLVGASGVLGRAVAAELGKRHDVVTAGSKSGEIRVD
                     IADPSSIEKAFAAAGALDAVACAAGSVNFEPLGAIKPASIEQSAYGLGLANKLMGQVN
                     LALAARDRLNDEGSITLIAGVLADQPIVAGSSASMVNGALESFVRAAAIELTRGLRIN
                     AVSPTVFAESMEGYGPFFRGFEAVPVARAALAFSRSIEGRETGKVYKVL"
     gene            5850..6521
                     /locus_tag="VKU03_07510"
     CDS             5850..6521
                     /locus_tag="VKU03_07510"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLJ71147.1"
                     /translation="MLDSRRGFYVTLALLVAVAGVFALWPNLDLDVAHLFYRDGGFVG
                     RGLGWTLGRDFFRVAPFIVLAAFAALYVCRRRGLTVPYAPSGRALAFLIATLAVGPGL
                     IVNLGLKDHAHRPRPSQTRDFGEHDEFRPWYRFDGACKKNCSFVSGEASQSFWMLAPA
                     SLAPPPLRPAALAAALAFGAVASLLRLAAGGHYLSDVLLAGLLTILIVQIGRWLAFRS
                     GDKAT"
     gene            6590..6718
                     /locus_tag="VKU03_07515"
     CDS             6590..6718
                     /locus_tag="VKU03_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71148.1"
                     /translation="MAYWFSRRARPHRPRFGFLALAAAVLLATTGYAVVLNGGWPA"
     gene            6840..8087
                     /locus_tag="VKU03_07520"
     CDS             6840..8087
                     /locus_tag="VKU03_07520"
                     /EC_number="6.3.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004055 - argininosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006526 - arginine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="argininosuccinate synthase"
                     /protein_id="HLJ71149.1"
                     /translation="MAQPDKSKIKRVVLAYSGGLDTSIILKWLQTTYNCEVVTFTADL
                     GQGEELEPARKKAEMLGVKPENIFIEDVREEFVRDYVFPMFRANAQYEGLYLLGTSIA
                     RPLIAKRLVDIAHKTGADAIAHGATGKGNDQVRFELSAYALDPDIKIIAPWREWGFAS
                     RNALIEFAEQNQIPIAKDKRGEAPFSIDANLLHVSSEGKVLEDPAQETPPYVYSRTVD
                     PEAAPDKPTVITVDFEKGDAVAINGEKLSPAALLAKLNQYGHDNGIGRLDLVENRFVG
                     MKSRGMYETPGGTILLTAHRGVESITLDRGAAHLKDELMPRYAELIYNGFWFSPEREM
                     LQALIDRSQEFVTGRVRLKLYKGNVILIGRESPYSLYDQDLVTFEEGAVAYDHRDAGG
                     FIKLNALRLRTLAKRNRKAKGRP"
     gene            complement(8152..8538)
                     /locus_tag="VKU03_07525"
     CDS             complement(8152..8538)
                     /locus_tag="VKU03_07525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015887492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ71150.1"
                     /translation="MRLLLDTHVLLWAAADSARLSAAARRLIGAAENERYFSAASLWE
                     LAIKRALGREDFQVDLAELREGLLANSYQELAVRSVHAISVTNLPPIHKDPFDRILVA
                     QAAVEGMTLLTADPLVAQYLGPVRKI"
     gene            complement(8535..8780)
                     /locus_tag="VKU03_07530"
     CDS             complement(8535..8780)
                     /locus_tag="VKU03_07530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004126824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLJ71151.1"
                     /translation="METFNIHHAKTHLSRLVDKAAKGEAFVIAKAGKPLVKVVPLETP
                     APQPIQPIGFLKGRIQIPADFDRMGEAEIGRLFDGDM"
     gene            8924..9157
                     /locus_tag="VKU03_07535"
     CDS             8924..9157
                     /locus_tag="VKU03_07535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020050887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLJ71152.1"
                     /translation="MKVTIHAAKTHLSKLIEAALAGEEVVIAKGEKPVVKLVPIEKHA
                     FKFGLLADKLTDPPPDFLEPMSEDELALWEGGD"
     gene            9154..9537
                     /locus_tag="VKU03_07540"
     CDS             9154..9537
                     /locus_tag="VKU03_07540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007539776.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ71153.1"
                     /translation="MNDLLLDTHAWAWSLGDDRRLSKMARARIAGASTILVSPISFYE
                     IAQKVRLGKWPQMASLVDSLPKRLLDQGGTIARLEPEICLRAGAMDWMHRDPFDRVLA
                     ATSLHFKIPLVSADSAFDGVVERIW"
     gene            complement(9545..>10134)
                     /locus_tag="VKU03_07545"
     CDS             complement(9545..>10134)
                     /locus_tag="VKU03_07545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176964.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HLJ71154.1"
                     /translation="EFVTRANLGRAYEWHYLDIGFGVTITGPHTVARMTNTLAIQPGE
                     KVLEIGTGSGYQSAYLSYLTDKIWTIEIIRPLAARTRAVYDGLIARGYSEYRAIAAKT
                     GDGYYGWADEAPFDKIIVTCGIDHIPPPLLQQLKPNGIMVIPIGPPGAQHVLKVVKRQ
                     SADGSIQVSRSDIYGGAIVNFVPLTQPGGGMHRAS"
BASE COUNT         1789 a         3244 c         3448 g         1653 t
ORIGIN      
        1 agccagcgct cgaacacggt ccgaatcgcg aagaaaattt ggcggcgaag ctgctccggc
       61 tcgatctggc tcagcgcggc gtcaggatcg ccgatgtcga ggacatggca ataaagcggc
      121 gagagctcgc gaatctcgtc cgcgccgagg ccgagctcgg ccaaggatgc ggcgagcctc
      181 gctttggctt cggccgcggg cgcgttacgg tcgatctgat gggcgctgcg cagcaccgcg
      241 gcgagcgcgc cgaaggactg ttcgccgagc ggcgagcaga cggcgatccg aacggcgatc
      301 ccggaaaagc gctcctcgtc gcgaaggcgg gcgaggaatt cgttgaccag ccgggttttt
      361 ccgatccccg cctcgccgat cagccgcacc acttgcgccg cgccgccgca ggcaaggtcg
      421 aggctgccga tcatatgggc gagttcggcg tcgcggccga tcagcggcgc gtgaaggccg
      481 atcgtctcca agccgcgcgc ggcgcgcggc ttttcaagcg gcccgatcag gcgatggacc
      541 agcacgctgc cggtcttgcc gcgcaaagac gcttcgccaa gcgcctcata agcgaaggca
      601 tggcgggtca ggcgataggt cagcggcccg accagcactt cgccccgcgc ggccgtggat
      661 tgcaggcgct gcgcggtgtt caccgtgtcg ccggtcaccg aataggattt ggcgctgccg
      721 gcgccgattc cgccggcgac gactgggccg gtattgacgc cgatatgcag ggcgagcggc
      781 gaaccgaggc gggcgcgcca attttcgccg aggcgcgcaa tccgttcggc catgtccagc
      841 gcggcgtgga gcgcgcgctc gggatcgtcc tcatgcgccg tcggcgcgcc gaacaaagcg
      901 agcaaggcgt cgccgatgaa cttgtcggga aagccgccga accggtgcaa ggcggcggtc
      961 atttcctcga aaatgtcgtt ctggagggcc tggaccagtt ccggatcgag ccgctcgctc
     1021 aaagtcgtga agccgctgag atcggcgaag agcaccgtga cggtgcggcg atcggcgtga
     1081 gcgtccatct tgacgggggc cgcctctgcg aagcgattgg gcggctcggc tcgccgcgga
     1141 gcggcggggg cggcgaccga ggccggcggg ctccgctcgc cggcctttgg cgcggcgccg
     1201 ggacttttgg cggcggcggt cgccgcgccg catttcgggc agaacgcgaa atccggcgcc
     1261 aaggcatagc cgcaggcggc gcattgcgcc ggctgcctgg cgccgcattg cggacagaag
     1321 gcgaagccgc tctgaatttc aaatccgcaa ccggcgcagg cgctcattcg cctcactccg
     1381 ccgccaggcg catcgtcttg cagcgcatca gcagagcccg caaagccgcc ggccgcaacg
     1441 gcttgttgag aatggcgaca tcggcgcgcg aagcagccgc gcgcatctcc ggatcgcgat
     1501 cggcggtcgc caggatcgcc ggcgtttccc cgccataggc ggcgcgcaac tcggcgatcg
     1561 cggcaaggcc gtcgccctcg tcgaggtgat agtcggcgac aatcgcgtcg ggcgcggcgc
     1621 cctcccgctt cagcgccgag aacgattctt caaggcttgc cgccgcaatg acgcggcaac
     1681 cccatttttc gagcaaagcg gtcaagccgc cgagcacgcg cggctcattg tcgatggcca
     1741 gcacgacaag atcggtcagc ggctcgtcgg cgcgcgccgc ctcgcggctg cgcggttcgt
     1801 ggcggggaag cgccttggcg aatgtcgcca gcggcgcggc gaccgagaac accgagccgg
     1861 cgcccggctt ggagcgaagc tggatgtcgt ggccgagcac gcgggcgagg cgctcgacga
     1921 tcgagaggcc gaggccgagg cccggcgccg cccgcgcgcc gcgatcgagg cgctggaact
     1981 cctcgaacac gatcttctgg ctcgcggcgg gaatgccgag gccagtgtcc cagacctcga
     2041 tggcgcagag cccgccgcgg cggcgacagc cgaccagcac gcggccttgc ggcgtgtatt
     2101 tgatcgcgtt ggagacgagg ttctgcaaca agcgccgcag catcagacgg tccgagcgta
     2161 cgatcagcga ggacgggaca aaggtgagct tcagcccttt ggcttcggcg atcggcgcaa
     2221 attcgatctc caattgccgc aaagggtcgg cgatcggaaa agcggcgacc tgcggcctgg
     2281 tcgcgccggc gtcgaggcgc gaaatgtcga gcagcgcgcc gagtatgtcc tcgaccgcct
     2341 ccagcgacgc gtcgacattg cgggcgagct cggccggctc gctctccttg gcccgcccgg
     2401 caatctcggc aagcgaggtc gcatagagcc gcgccgcgtt gagcggctgc aaaatgtcat
     2461 gactggcggc ggcgagaaag cgcgtcttcg acagattggc gtcttcggcg gcgttcttgg
     2521 cgcgagccag ctcctggttg agccgctcca attgctcggt gcgctcgcgg acgcgctgct
     2581 ccaattgctc gttgaccgcg gcgagccttt cttcggcgtg aaccgcctcg gtcacgtcgg
     2641 tataggtggt gacgacgccg ccatccggca ggcgcgccga tcgtacttcc gccaccaggc
     2701 ccttcggata gaggcgaagg cgcgacggcg cctggtcgtt gagcaggctc tcgatccggc
     2761 tggcgacgaa ctcgtcggaa gcgccggggc catagacgcc gcgggcggcg ttgaagcgga
     2821 cgatctcgtc gaggccgacg ccgagccgca gcagcggatt gggcagatcg aacaattcgc
     2881 cgaaggcccg gttccagcag gtcaaggcaa gattgcggtc gaacacggcg atcgcctggc
     2941 gggcgtgatc gagcgcatgt tgaagcaaat cgcggttgga ctggatggcg gcggaggcgt
     3001 cgtcgatcaa tcgcagcgcc gccttgctcg acatcgcgcg gcggcggaac aggagcgaca
     3061 tgaccaggcg cgacgacgag gcgccgatcg ccgaggcgag cagatgctcg gcatggcgga
     3121 tgagcggcgc gtcggcttcg ccggcgggct cgaatttgag gccgcgtccg cccatgaagc
     3181 tttcaagcgc atggcgggcg cggtcggcgc cgagatagag cgagaccgtc gcttcgagct
     3241 cgccggcggt ggtcgaagcg cgccacagcc gaaggctggg cgctttcgcc gcgggctcgg
     3301 cgccgacgaa agcgcccgcc tgcagccgct cgatcgcatt ggcttcgcgg gtcagcgaga
     3361 agagtacgaa cgcgataaga ttggcgccga ggctgagaac cgcgccgctg accaatggcc
     3421 gcccggtgat ggcgagcaaa gcgctcggct tcagccaggc gatcgatagc gggccctgtt
     3481 cgagaaaatt gacgaaagcc gacgaattga ggtcgagcga cggcaaaagc agcgtgtaga
     3541 accagatcgc ggcgccgatg atcaggccgg ccgaggcgcc gcgggcgttg gcccgccgcc
     3601 agaacaggcc gccgagaaag gccggcgcga tctgggcgat gccggcgaag gacaacaacc
     3661 cgatcgacga cagcgccgcg tcgttggcga tgcggatata ggcgtagccg agaaacagca
     3721 cgccgaggat cgcggcgcgg cgcacgaaca gcaccagcga gccgatcgcg ccttcctggg
     3781 cggcggcgcg ggcgcggccg cgcaggatga gcggcatgat cagattgttc gagacgctga
     3841 tcgacagcgc gacgctttcg accacgacca tcgcggtcgc cgccgacagc ccgccgatca
     3901 tcgtcaagag ggcgacgcca tgggcgccgg ccgcgagcgg caaggcgagg accgtatagt
     3961 cgcggtcgat cgtcccggcg ctgaacgtcg ccaggccggc gatcgccagc ggcaccacga
     4021 acaaattgat cgccgcgaga taaagcgtga agcgccaggc ggcggcgggg atgtcgcgct
     4081 cgtcgcggtt ctcgacgatc atcacatgga actggcgcgg cagcagcaga atggcgaccg
     4141 ccgacaacag cgtggtgacg atccaattca gcggatcggg cggggtgcgc ataatcgcgg
     4201 cgatcgcggc attgtcggcg gcgatgtgag cgaggcggcc gaggccgccg aacaggcccc
     4261 agacgacgaa ggagccgacg ccgagaaagg cgacgagctt gaccagcgac tcggcggcga
     4321 cggcgaggat caagccgtct tgatgctcgg tcgcctcgat ccgccgcgtg ccgaacgcca
     4381 tggcgaacgc ggccaggaca agggcgacgg cgagaaagaa aatcggggag ggcgccgccg
     4441 gcgcgagccg gccctggtcg agcgagccga ccatcatcag gatggtctgg gcgatcgcct
     4501 tgagctgcag cgcgatatag ggcgccgagg cgatcatcgc gacgaaggcg accaaagcgg
     4561 ccacatgctg cgccttgccg tagcgcgccg cgacgaaatc ggcgaccgag gtgaggttct
     4621 gccccttggc gagcgccgcg atccgcgcca ccagccggtg gccgaggccg acgaccagga
     4681 ccgggccgat atagatgggc agaaaatcca gcccatgcgc cgaggcgatg ccgaccgagc
     4741 cgtaataggt ccaggtggtg caatagaccg cgaggctcag cgcatagacc gcgccgcgcg
     4801 cccggccggc catgaagcgg cggccggacc ggtcgcccca ataggcgatc aggaacagcg
     4861 ccccgacata ggcgatcgcg gtggcgaaga cggtcacgcc gtcaagcatc ggcggcctgg
     4921 tcctggtctc gggtgagcgt gctggccgct cggggctcac cctcacccca gccctctccc
     4981 gcaagcggga gagggggcgc gccgaccgtt tcatcggcgc tcgtgaaccc cttctcccgc
     5041 ttgcgggaga aggtggcgcg aagcgccgga tgagggtcaa ccgcggcggc gatcatgtga
     5101 cgcaactcag aaagccctac tgagccagtc tagcctattt tgacgcccag cggacgggac
     5161 tttcattttc cggcgcccta tgcaacgaac cggccgtcca aggccggaag ggacccgtta
     5221 tggcggagaa aagattgcgg attttgctgg tcggggcgag cggggtgctg ggccgcgccg
     5281 tcgccgccga gctcggcaag cgtcatgacg tcgtgaccgc cggctccaaa tcgggtgaaa
     5341 tccgcgtcga catcgccgat ccgtccagca tcgaaaaggc cttcgcggca gccggcgctc
     5401 tcgacgccgt cgcctgcgcc gcaggttcgg tcaatttcga gccgctcggc gcgatcaagc
     5461 cggcgtcgat cgagcaatcg gcctatgggc tgggcctcgc caacaagctg atgggccagg
     5521 tcaatttggc gctggcggcg cgcgaccggc tcaatgacga gggctcgatc acgctgatcg
     5581 ccggggtgct ggccgaccag ccgatcgtcg ccggctcttc ggcgagcatg gtcaacggcg
     5641 cgctcgaatc cttcgtccgc gccgcggcga tcgaattgac gcgaggcttg cgcatcaatg
     5701 cagtgagccc gacggtcttc gcggagtcga tggagggcta tgggccgttc ttccgcggct
     5761 tcgaggcggt tccggtcgcg cgcgcggcgc tcgccttcag ccgcagcatc gaagggcgcg
     5821 agaccggcaa ggtctacaaa gtgctgtaga tgctcgattc gcgccgcggc ttctatgtca
     5881 cgctcgcgct cctggtcgcg gtcgccggcg tctttgcgct ttggcccaat ctcgacctcg
     5941 acgtcgccca tcttttttac cgcgacggcg gctttgtcgg gcgggggctc ggatggacgc
     6001 tcggccggga cttctttcgg gtcgcccctt tcatcgtgct tgccgctttc gccgcgctct
     6061 acgtctgccg ccggcgcggt cttacggttc cctatgcgcc gagcggacgc gcgctcgcct
     6121 tcctgatcgc gaccctcgct gtcggccctg ggctcatcgt caatttgggg ctcaaggacc
     6181 atgcccatcg gccgcggcct agccagacgc gggatttcgg cgagcatgac gaattccgcc
     6241 cctggtatcg ctttgacggg gcttgcaaga aaaattgctc cttcgtcagc ggcgaggcct
     6301 cgcaaagctt ctggatgctc gccccggcga gcctggcgcc gcctccgttg cgtcccgcgg
     6361 cgctcgccgc cgcgctcgct ttcggcgccg tcgcgagcct gcttcggctg gccgcggggg
     6421 gccattatct gtccgatgtc ctcctggcgg gcctgctgac catcctcatc gttcagatcg
     6481 ggcgctggct cgcgttccgc tcgggcgata aggcgacctg agggcgcgcg ctggcgggga
     6541 acaatccagg cctcgtggcg ttcgccattg gtcaatagag gcgaaggcga tggcgtattg
     6601 gttttcgcgc cgggcgcggc ctcaccggcc gcgcttcggg tttttggcct tggcggcggc
     6661 cgtgctcctt gcgacgaccg gctatgccgt cgtcctcaac ggcggctggc ccgcctaaat
     6721 cctcatcgat tcatcgtcgc ccggccgcga cccggagagg gttgcgcgcc agacattgcg
     6781 ccctgtagaa gccctgcgag ccgtttacgt ccatcattcg ctcgaagagg cttaagggga
     6841 tggcccagcc cgacaaatcg aaaatcaagc gcgtcgtcct cgcctattcg ggcgggctcg
     6901 acacgtcgat catcctgaaa tggctgcaga cgacctacaa ttgcgaggtc gtcaccttca
     6961 ccgccgatct cggccagggc gaggaactgg agccggcgcg caagaaggcc gagatgctcg
     7021 gcgtcaagcc ggagaatatc ttcatcgaag acgtgcgcga ggaattcgtg cgtgactacg
     7081 tcttcccgat gttccgcgcc aatgcgcaat atgaaggcct gtacctgctc ggcacctcga
     7141 tcgcccggcc gctcatcgcc aagcgcctcg tcgacatcgc gcataagacc ggcgccgacg
     7201 cgatcgccca cggcgcgacc ggcaagggca acgaccaggt gcgtttcgag ctttcggcct
     7261 atgcgctcga tccggacatc aagatcatcg cgccgtggcg cgaatggggc ttcgcctcgc
     7321 gcaacgcttt gatcgagttc gccgagcaga accagattcc gatcgccaag gacaagcgcg
     7381 gcgaagcgcc cttctcgatc gacgccaatc tcctgcacgt ctcctcggaa ggaaaggtgc
     7441 tcgaagaccc ggcgcaggag acgccgccct acgtctattc gcgcaccgtc gatccggaag
     7501 ccgcgcccga caagccgacc gtcatcacgg tcgatttcga gaagggcgac gcggtcgcga
     7561 tcaatggcga aaagctgtcg cccgccgcgc tgctggccaa actcaaccag tatggccacg
     7621 acaacggaat cggtcggctc gacctcgtcg agaaccgctt cgtcggcatg aaatcgcgcg
     7681 gcatgtacga gacgccgggc ggcacgatcc ttttgaccgc gcatcgcggc gtcgagtcga
     7741 tcacgctcga ccgcggcgcg gctcatctca aggacgagtt gatgccgcgc tacgccgaac
     7801 tgatctataa cggcttctgg ttctcgcccg agcgcgaaat gctgcaggcg ctgatcgaca
     7861 ggagccagga attcgtgacc ggaagggtgc ggctgaagct ctacaagggc aacgtgatcc
     7921 tgatcggccg cgagagtccg tactcgctct acgaccagga tctcgtgaca ttcgaagaag
     7981 gcgcggtcgc ctacgaccac cgcgacgccg ggggcttcat caagctgaat gcgttgcgat
     8041 taaggacgct ggcgaagcgg aacaggaagg cgaagggacg tccatagttg cgggcagatc
     8101 gcgagagccg tcaaatcatc gatatcgggt gatgttaaac cgcacttctt tctaaatttt
     8161 cctgaccggc cccagatatt gcgccaccag cgggtcggcc gtaaggagcg tcatgccttc
     8221 gactgcggct tgcgcgacaa gaatgcggtc gaacgggtct ttatggatcg gcggcaggtt
     8281 cgtcacggaa atggcgtgga cgctccgcac ggctaattcc tgataggaat tggcgagcaa
     8341 gccttcgcgc agctccgcaa gatcgacttg aaagtcctcg cggccgagcg cgcgcttgat
     8401 cgccaactcc cacaggctcg cggcgctgaa gtaccgctcg ttctcggcgg cgccgatcaa
     8461 ccggcgagcg gccgccgata gacgcgccga atccgccgcc gcccaaagca gaacatgcgt
     8521 gtcgagaaga agtctcacat gtcgccgtcg aacaagcgtc cgatctcggc ttcccccatg
     8581 cgatcgaaat cggcgggaat ctggatgcgc cccttcagga agccgatcgg ctggatcggc
     8641 tgcggcgcgg gcgtttccag gggcacgacc ttgaccaggg gcttacctgc cttggcgatg
     8701 acgaacgcct cgcccttggc cgccttgtcg accaggcggg aaagatgagt cttggcgtgg
     8761 tggatgttga aagtttccat cgagagatca tcggacttag ttcgataaac ttagtttatg
     8821 tcacgcccag tcgcaagctc ttctgtgcat tcccggcaag cgtctcttag ctaacttgac
     8881 taactgcggg atcaggctca tttaggccgc caggagagcg cgaatgaaag tcaccatcca
     8941 cgccgccaag acccaccttt ccaagctgat cgaggccgct ctagcgggcg aggaggtcgt
     9001 gattgcgaaa ggcgagaagc cggtcgtcaa attggttcct atcgaaaaac acgctttcaa
     9061 gtttggcctg cttgcagaca agctgaccga tccgcccccc gacttcctgg agccgatgag
     9121 cgaggacgaa ctcgcgcttt gggaaggtgg cgattgaacg atttgttgct cgatactcac
     9181 gcctgggcgt ggagcctagg cgatgatcgg cgactctcga agatggctcg ggcgcggatc
     9241 gccggagctt caactattct tgtcagtccg atctcttttt acgaaattgc gcagaaggtc
     9301 aggctgggca aatggccgca aatggcgtcc ctcgtcgaca gcttgccgaa acgacttctc
     9361 gatcagggcg gaacgatcgc gcggctggag ccggagatct gtttgcgcgc tggcgcgatg
     9421 gactggatgc atcgcgatcc attcgaccgc gtgcttgccg cgaccagttt gcacttcaaa
     9481 attcccctcg tctcagccga ctccgctttc gacggagtcg tcgagcgcat ctggtgaagc
     9541 ccggttagct cgcccggtgc atgccgccgc ccggctgggt gagcggcacg aaattgacga
     9601 tcgcgccgcc atagatgtcg gagcggctga cttggatcga accgtcggcg ctttgccgct
     9661 tgaccacttt cagcacatgc tgcgcgccgg gcgggccgat cgggatgacc atgattccgt
     9721 tcggcttgag ctgctggagc agcggcggcg ggatgtggtc gatgccgcag gtgacgatga
     9781 tcttgtcgaa gggcgcttca tccgcccagc cgtaatagcc gtcgccggtc ttcgccgcga
     9841 tcgcccgata ttccgaatag ccgcgcgcga tcaggccgtc atagacggcg cgggtgcgcg
     9901 cggcgagcgg ccggatgatc tcgatcgtcc agattttatc ggtgaggtac gacagatagg
     9961 ccgactggta gcctgaaccg gtgccgattt cgagcacctt ttcgcccggc tggatggcga
    10021 gcgtgttggt catccgcgct acggtatgcg gcccggtgat cgtcacgccg aagccgatat
    10081 cgagatagtg ccactcatac gcccggccga gattggcgcg ggtgacgaac tcct
//
LOCUS       DATGJW010000244        10452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_771753, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000244 DATGJW010000000
VERSION     DATGJW010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10452
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_771753"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VKU03_07550"
     CDS             <1..460
                     /locus_tag="VKU03_07550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLJ71155.1"
                     /translation="WAAAHGLKIIFAFPGVAIATAFVTFPFVARELIPVMQQQGSAEE
                     EAALTLGASGLRAFVTITLPNVKWALLYGVLLCNARAMGEFGAVSVVSGHIRGLTNTI
                     PLQVEILYNEYNLVAAFALASLLAGLALVTLAIKVLLERRHADDLAERGR"
     gene            429..1235
                     /gene="cysA"
                     /locus_tag="VKU03_07555"
                     /pseudo
     CDS             429..1235
                     /gene="cysA"
                     /locus_tag="VKU03_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019444591.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015419 - ABC-type sulfate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008272 - sulfate transport [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate ABC transporter ATP-binding protein"
     gene            complement(1259..1528)
                     /locus_tag="VKU03_07560"
     CDS             complement(1259..1528)
                     /locus_tag="VKU03_07560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71156.1"
                     /translation="MKSARLALLAILFTAGAANAQGGPSDRGAPPSVVGRYSATSRQS
                     LGQAPSGERIIRNANDCAPDKATPVWGADSSLIGYSCAPYSANGS"
     gene            complement(1832..2584)
                     /locus_tag="VKU03_07565"
     CDS             complement(1832..2584)
                     /locus_tag="VKU03_07565"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S1 family peptidase"
                     /protein_id="HLJ71157.1"
                     /translation="MFRCSSVGLVLAALLSAAAPAFALVGPAEDDHSFAPHLVMILNR
                     GLDRAGFCTGVVIAPRVVLTAAHCVVSADNMRVYYRGPNGEPVLREIAATAIHPRFHP
                     DAPAKRIVSIDLALVETRAPLDERFTPAPLDDRGAVAVGEALKIFGYGVAREGDGKTA
                     GSLRAARLAARAPQSSILLWAEDPDHAGAGACTGDSGGPIVSAESGKVLAITAWSAGA
                     GHGRRCGALTQGPLVAPQAGWIRGVVEHWSER"
     gene            2826..3179
                     /locus_tag="VKU03_07570"
     CDS             2826..3179
                     /locus_tag="VKU03_07570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PepSY domain-containing protein"
                     /protein_id="HLJ71158.1"
                     /translation="MARSFLFSLACVVFAVSRALAAEAAAAAVRPVCLNGSETRAEIA
                     AHRLLQPFAVLKSAAAIAKAEALSAKLCKIGEEFVYEIALLHHDGRLVHLVMNAATGK
                     VLNPRSLRELPPPRT"
     gene            3186..3881
                     /locus_tag="VKU03_07575"
     CDS             3186..3881
                     /locus_tag="VKU03_07575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLJ71159.1"
                     /translation="MRLLVVEDDRDLNRQIVAALEGAGYAVDRAYDGEDGWFLGDTEP
                     YDAVVLDIGLPKRDGVAVLEAWRRAGRTMPVLILTARDRWSDKVQGFDAGADDYVAKP
                     FHMEEILARLRALLRRAAGHATNELVAGPVRLDARTGRVTVNGAPIKFTSHEYRLLAY
                     LMHHAGRIVPRSEIVEHLYDQDFDRDSNTIEVFVGRLRKKLGVDAIQTVRGLGYLVPT
                     GGEGDGGSDKSAR"
     gene            3906..5303
                     /locus_tag="VKU03_07580"
     CDS             3906..5303
                     /locus_tag="VKU03_07580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ71160.1"
                     /translation="MNALRAQSIASRLFISAAFWSFTILLIAGVGLSAINRRSTEASF
                     DERLGVYLKALVVNVANSSDESRAGSAQLIDPEFNLARSGWYWQITRLDAGKSNTGQP
                     EIRTSPSLAASLLPRLETNIPPAEEGAPHAGYVKGPDDRRLRLVERVIDAGDEGRYLI
                     QVAANAEEIESDIENFEYALGATFLLLAIALIGSMALAVRFGLSPLRALQEGVVAIRR
                     GEAEQIKGDFPQDIAPLAAEVNLLLEANREVVERARTQVGNLAHALKTPLSILINEAE
                     AGSPMLAEKAKEQAEIMRHQVAFYLDRARAAARANALGSATEVKPVIEGLTRTFQKVY
                     RDRALAFSIDAPDGLRFRGEEQDLTDLIGNLLDNAGKWAHERIDIHAARDPRSDSSGR
                     PYFITMIDDDGPGLDPQARAEALRRGKRLDESRPGSGLGLSIIVDLAHLYGGALQLED
                     SPLGGLRATLRLPAS"
     gene            5362..5766
                     /locus_tag="VKU03_07585"
     CDS             5362..5766
                     /locus_tag="VKU03_07585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71161.1"
                     /translation="MACLAAAALAGCSTAQSAPPPAAAMAAGPAVAAGPPPTPETVDP
                     SMLGGVLAGSVGMGLENSDRIAAYKAQVAALATGQRSSWRGERGVYGYVEAGPAQGSC
                     RSFSQTVYIAGRPYTGSGAACRQSDGSWRVEG"
     gene            5778..6638
                     /locus_tag="VKU03_07590"
     CDS             5778..6638
                     /locus_tag="VKU03_07590"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ71162.1"
                     /translation="MAKAEDAMQGKVAVMTGATSGIGEVAAERLAEAGATILFVARDQ
                     RRAEATLARLAAKNRAPHRFFRADLSNLAETKRVGAAIAAEAPRIDVLVNNAGAMFSS
                     RRETADGLEMTFALNHMSYFVLTAALLEKLKASAPARIVSTASAAHQGARLDFDDLQN
                     KRNYSGMRAYGRSKLCNILFTRALARRLQGTGVTANCLHPGFVATRFGDEAGGAVGLL
                     IPLAKLFGRRPEKGAETIVYLASSPDVASESGFYYFECRKTTPSAAAQDDEAARQLWT
                     ASEKLAGFVS"
     gene            6827..7945
                     /gene="ccmI"
                     /locus_tag="VKU03_07595"
     CDS             6827..7945
                     /gene="ccmI"
                     /locus_tag="VKU03_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome biogenesis protein CcmI"
                     /protein_id="HLJ71163.1"
                     /translation="MIWVVFALMTGAAVLCALWPLARKRASAEGETRAVAFYKAQIAE
                     IDRDVARGQLPQAEAEGARIEASRRLIAASERAERGGGAARPAPRLAAALLILIGVPL
                     VAFGLYGRYGNPGEPDEPIAARLNDPAHGDDLQAAVAKIESHLLTHPNDGVGFKVIAP
                     AYLRMGRFDDAVKAYGQALRLLGDRPELRADYGEALVAAANGVVTADARVAFQQALAK
                     DPRSAKARYYLALAAEQDGDKQRAGETYKALLAESPNAPWAPTVRMRLAALGEMAPAA
                     TAKIAAPPKEEAAAIASLSPEQRQAAIHGMVDRLAARLAAKGDDPAGWLRLIRAYSVL
                     QEQDKAKAALAKAREALNGDAAAKRDLDALAKELGLES"
     gene            7948..8394
                     /gene="ccmE"
                     /locus_tag="VKU03_07600"
     CDS             7948..8394
                     /gene="ccmE"
                     /locus_tag="VKU03_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HLJ71164.1"
                     /translation="MTRKGRRLTLIGVALCVIAFAAGLALYALRDSIVFFYGPSELAE
                     KDVRPGAHLRVGGLVKPGSLVKAADGKMSFVITDNAHDVAVTYQGQVPDLFREGQGVV
                     AEGVLEAPGKFHADTILAKHDERYMPREVVDALKKQGRWQEGGGGS"
     gene            8391..10373
                     /locus_tag="VKU03_07605"
     CDS             8391..10373
                     /locus_tag="VKU03_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HLJ71165.1"
                     /translation="MIVEAGHYALTLALALALVQAFAPLWGAAHRDEGLIGVAQSTAI
                     GQFVAIAFSFGALIEAHLVSDFSVLNVAENSHTAIPTLYKITGVWGNHEGSMLLWVFI
                     LALFGALVAAFARALPPVLRGNVLAVQGLISAAFLAFILITSNPFARLDPAPFEGRDL
                     NPVLQDLGLAIHPPLLYLGYVGFSIVFSFAAAALIEGRVDAAWARFVRPFALIAWSFL
                     TLGIAMGSYWAYYTLGWGGFWFWDPVENASLMPWLAGTAFVHSIAVMEKREALKVWTI
                     FLAIVAFSFSLLGTFLVRSGVLTSVHAFASDPQRGVFILLILAAFIGGALALFAWRAP
                     LLKQGGLFAPVSREGALVLNNLFLTTSCAAVMIGTLYPLALEQMTGEKISVGAPFFNM
                     TCGALLIALCAIVPFGQSLAWKRGDLLGVAQRLAVAGAAGLCIAAALLTLTSGGPVLA
                     PLGAGLAVYVILGALSEIAARLWRGGASARIALARAWGLPLSAWGAAFAHIGVGVTLL
                     GLAMTGFGAETIATMKIGEPRAVGPYLVVFESAQPRLGPNYRETVAKAAIRKDGVVVA
                     TVEPAKRFFPARETSTTEAGIATLDLGQVYISIGDETGGAIAARLYWKPLVTLIWIGA
                     CIMALGGALSLADRRLRIGAPARAKGAIAPQAAGAQ"
BASE COUNT         1615 a         3503 c         3477 g         1857 t
ORIGIN      
        1 gtgggcggcg gcgcatggct tgaaaatcat cttcgctttt ccgggcgtcg cgatcgcgac
       61 ggccttcgtc acctttccgt tcgttgcgcg cgaactcatc ccggtgatgc agcagcaggg
      121 atcggcggaa gaggaagccg cgctcactct tggcgcaagc gggctgcgcg ccttcgtcac
      181 gatcactctg cccaatgtca aatgggcgct gctctacggc gtgctgctct gcaacgcccg
      241 cgccatgggc gaattcggcg cggtctcggt ggtttccggc catatccgcg gccttaccaa
      301 cacgatcccg ctgcaagtgg agattctcta caatgagtac aatctcgtcg ccgccttcgc
      361 gctcgcctcg cttctcgccg gcttggcgct ggtcaccctc gcgatcaagg tcctcctcga
      421 acggcgccat gccgacgatc ttgccgagcg cggacggtag gagccgcgcg gaaaaggaaa
      481 ccgcgccggt cgcgccgccg gttcggtccg gccgcgccgg cgcacaggag atcgcgcttg
      541 tcggcgtcgg caaggcgttc ggcgtcgagg cggcgctgag ggacgtcaca ttgaccattc
      601 agcccggcga attgctcgcc ttgctcggcc cgtcgggctc gggcaagacc accctcttga
      661 gaatcatcgc cggattggag cgtgccgagg gggaggtccg gttcggcggc gaggatgctt
      721 cgcgcctgag cgtccaggag cgccgcgtcg gctttgtctt ccagtcgtac gcgctgtttc
      781 ggcatatgag cgtgttcgac aatgtcgcct acggcctgac ggtccggccg cgccgcgccc
      841 gccctgcgcg gcgcgacatc gccgagcgcg tgcggcgatt gctggacctc gtgcaattga
      901 gcgaggtcgg ccggcgctat cctgcacaat tgtcgggcgg tcagcgccaa cgcgtcgcgc
      961 tcgcgcgcgc cttggcgatc gagccgaagg ttctgctgct cgacgagccg ttcggcgcgc
     1021 tcgacgcgaa ggtgcgcaag gagctgcgcg gctggctccg cgaaatccac gacaggaccg
     1081 gcctgacgac ggtgttcgtc acgcacgatc aggccgaggc gatggagctg gccgaccgcg
     1141 tcgccatcct gaaccggggc gcgatcgagc aagtcggctc gccgacggaa attatcgagg
     1201 cgccggcgac gccgtttgtg gcgtcgtttc tgagctgatc ttgcgcgtca aatcgacgtc
     1261 aagacccgtt cgcgctgtaa ggggcgcagg aatagccgat aagcgaagaa tcggcgcccc
     1321 agacaggcgt cgccttgtcg ggcgcgcaat cgttggcgtt gcgaatgatc cgttcgcccg
     1381 aaggcgcctg gcctaacgat tggcggctcg tcgcggaata tcggccgaca acggacggcg
     1441 gcgcgcctct gtcgcttggg ccgccctgcg cgttggccgc accggcggtg aaaagaatgg
     1501 cgagcagggc gaggcgggca cttttcatcg cacgctcttt cggcaacgag ttgttcgcac
     1561 agatgctctc gctcggctca cggcgccgac gaaacgcggc gttcccgagt gaggcgatcc
     1621 gcgattcgga cgctcaggct cgctcaatcg gcaagcgcag cagtgcggac gtgcggcgac
     1681 tgtttcaatt aatatttttt ttatgcgccg tgtccgatcg cagaagaagc ttcaaaggta
     1741 cgcaaaaccg taccattttg cgcagcgagg gcaggccggc gcagcggcgg cgcgatcctt
     1801 gacaataaac aagtgttgat tgtgattagt tctaacgttc gctccaatgt tccacgacgc
     1861 cgcgtatcca gcccgcttgg ggcgccacca gcgggccctg ggtcagcgcg ccgcagcgcc
     1921 ggccatggcc ggcgccggcc gaccaggcgg tgatggcgag caccttgccg ctctcggccg
     1981 agacgatggg gccgccagaa tcgccggtgc aggcgccggc gccggcgtgg tccgggtcct
     2041 ccgcccaaag cagaatggac gattgcggcg cgcgcgccgc gagcctcgcg gcgcggagcg
     2101 atccggcggt tttgccgtcg ccttcccgcg ccacgccata gccgaaaatc ttcagcgcct
     2161 cgccgaccgc caccgcgccc ctgtcgtcga gcggcgccgg ggtgaagcgc tcgtccagcg
     2221 gcgcgcgggt ctcgaccaga gcgagatcga tcgacacgat tcgcttggcc ggggcgtcgg
     2281 ggtgaaagcg cggatggatc gccgtcgccg cgatctcgcg caacaccggc tcgccgttcg
     2341 ggccgcggta atagacgcgc atattgtcgg ctgagacgac gcaatgggcg gcggtcaaca
     2401 cgacccgcgg cgcgatcacc acgcctgtgc aaaagccggc ccgatcgaga ccgcggttga
     2461 ggatcatgac cagatggggg gcgaaggaat ggtcgtcctc ggccgggccg accagcgcga
     2521 aggcgggcgc cgccgcgctt aacaaagccg ccaagacgag cccgaccgaa gagcatcgaa
     2581 acatctgccg ccacgtcctc attgcttcaa agatcgggta gtctttgtga cgggcctcgc
     2641 gcaagctctc ccgcggggag cgatcacgcg cttgtcagtc ggagccgcta ttcttcgagg
     2701 cgcagaaaat gcgacaggag aggattgggg caaagagcgt tccagagggc gggcgagccg
     2761 tcgcccgctc ggcgcggggc gccgccattc attcgtcgtt catgcggccc gggcgaggtt
     2821 tcttcatggc gcgaagcttc ctcttttcgc tggcttgcgt tgttttcgcg gtctcgcgcg
     2881 cgcttgccgc ggaagcggcg gccgcggcgg tgcggccggt ctgcctgaac ggaagcgaaa
     2941 cccgcgcgga aatcgccgcc catcgcctgc ttcagccttt cgcggtgctc aaaagcgccg
     3001 ccgcgatcgc caaggctgag gcgctttccg ccaagctgtg caagatcggc gaggagttcg
     3061 tctacgaaat cgccctgctg caccatgacg ggcggctcgt ccatttggtc atgaacgccg
     3121 ccacgggtaa ggtcctcaac ccgcgcagct tgcgcgaact gccgccgcca aggacttgaa
     3181 agctcatgcg tctgctcgtc gtcgaagacg accgcgacct caaccgacag atcgtcgccg
     3241 cgctggaagg ggcgggctac gccgtcgatc gcgcctatga cggcgaggac ggttggttcc
     3301 tcggcgacac cgagccttac gacgccgtgg tgcttgatat cggcctgccg aagcgcgacg
     3361 gcgtcgccgt cctcgaagcc tggcggcgcg ccgggcgcac catgccggtc ctgatcttga
     3421 cggcccgcga ccggtggagc gacaaggtcc aggggtttga tgcaggcgcc gacgattatg
     3481 tcgccaagcc gttccatatg gaggagattc tggcgcggct gcgcgccctt ctgcggcgcg
     3541 ccgcgggcca cgccaccaac gaactggtcg cgggcccggt gcgcctcgac gcccgcaccg
     3601 gccgcgtcac cgtcaacggc gcgccgatca agttcacctc ccatgaatac cgcctgctcg
     3661 cctatctcat gcatcacgcc gggcgcatcg tgccgcgcag cgagatcgtc gagcatctct
     3721 acgatcagga tttcgaccgc gattcgaata cgatcgaggt ctttgtcggg cgcctgcgca
     3781 agaagctcgg cgtcgatgca atccagacgg tgcgcgggct tggctatctg gtgccgaccg
     3841 gcggcgaggg ggacggcggg tccgacaaat cggcgcgctg aggccctatc ggcgatgagg
     3901 agaagatgaa cgcgctgcgc gcgcaatcga tcgcttctcg cctgttcatc tcggcggcgt
     3961 tctggagctt cacgattctg ctcatcgccg gcgtggggct ctcggcgatc aaccgtcgct
     4021 cgacggaggc gtccttcgac gagaggctcg gcgtctatct caaggcgctg gtcgtcaatg
     4081 tcgccaattc cagcgacgaa tcgcgggccg gatcggcgca attgatcgat ccggaattca
     4141 atctcgcccg ctccggctgg tattggcaga tcactcggct cgacgccgga aagagcaata
     4201 ccggccaacc ggaaatcagg acctcgccct cgctcgccgc ctcgctgctg cctcgtctgg
     4261 aaacgaatat cccgccggcc gaagaagggg cgcctcacgc cggctatgtg aaaggccccg
     4321 acgaccggcg cctgcgcctc gttgaacggg tgatcgacgc gggcgacgaa ggccgctacc
     4381 tcatccaagt ggcggcgaac gccgaggaga tcgaatccga cattgagaat ttcgaatatg
     4441 cccttggcgc gacgttcctt ttgctcgcca tcgccttgat cggctcgatg gccctcgcgg
     4501 tgcgtttcgg cctttcgccg ctgcgcgcgc tccaagaagg cgtcgtggcg ataaggcgcg
     4561 gcgaagccga gcagatcaag ggcgattttc ctcaagatat tgcgccattg gccgccgagg
     4621 ttaatctctt gctcgaagcc aatcgcgagg tcgtcgagcg cgctcgcacg caggtcggca
     4681 atctcgccca cgccttgaag actccgctca gcatcctgat caacgaagcc gaggccggca
     4741 gtccgatgct cgccgagaaa gccaaagagc aggccgagat catgcgccac caggtcgcct
     4801 tctatctcga tcgggcgcgc gcggcggcgc gcgccaacgc gctcggctcg gcgactgagg
     4861 tcaagccggt gatcgagggc ctgacgcgca cattccagaa agtctatcgc gaccgcgctt
     4921 tggccttttc aatcgacgcg ccggacggat taaggtttcg cggcgaagag caggacttga
     4981 ccgacctcat cggcaatctg ctcgacaatg ccggcaaatg ggcgcatgag cgtatcgata
     5041 tccatgcggc gcgcgacccg cgctcggatt ccagcggccg gccctatttc atcactatga
     5101 tcgacgatga cggaccgggg cttgatccgc aggcgcgggc cgaggcgctg cgccgcggca
     5161 agcggctcga cgaatcgcgg ccaggctccg gcctcggcct gtcgatcatc gtcgacctcg
     5221 cccatctcta tggcggcgct ttgcagctcg aagacagtcc gctcggcggc ctacgcgcga
     5281 cgttgcgatt gccggcttcc tagagatttt gacgaagaga agatttcgga gggtattccg
     5341 atggtttggc ggggagttct aatggcgtgt ctcgccgcgg cggctctggc tggctgctcc
     5401 acggcgcaaa gcgcgccgcc gccggcggcc gccatggccg cggggccggc ggtcgctgcc
     5461 gggccgccgc cgactcccga gacggtcgat ccctcgatgc tcggcggcgt gctcgctgga
     5521 tcggtgggga tgggcctcga aaacagcgac cgcatcgccg cgtataaggc ccaggtcgcg
     5581 gcgctggcga cgggccaacg ctcgtcctgg cgtggcgagc gcggcgtcta cggctacgtc
     5641 gaggccggcc cggcgcaagg ctcgtgccgc tccttctcgc aaaccgtcta cattgccggc
     5701 cggccctata cggggagcgg cgcggcctgc cgccagtccg acggctcatg gcgcgtcgag
     5761 ggctgacgcg ccccaatttg gcgaaagcgg aagacgcgat gcagggcaag gtcgcggtga
     5821 tgaccggcgc gacgtccggc atcggcgaag tcgccgcgga gcggctcgcc gaagccggcg
     5881 cgacgatcct cttcgtcgcc cgcgatcagc ggcgggcgga ggcgaccttg gcgcgccttg
     5941 cggcgaagaa ccgcgcgccg catcgcttct ttcgcgccga tctttcgaac cttgccgaga
     6001 cgaagcgggt cggcgccgca atcgccgccg aggcgccgcg catcgacgtt ctcgtcaaca
     6061 atgccggagc gatgttctct tctcgacgcg agaccgccga cggcttggag atgactttcg
     6121 cgctcaacca tatgagctat ttcgtgttga ctgcggcgct gctcgaaaaa ctcaaggcct
     6181 cggcgcccgc tcggatcgtc agcaccgcct cggccgcgca tcagggcgcc cggctcgatt
     6241 tcgacgacct gcagaacaag aggaattaca gcggcatgcg cgcctatggc cgctcgaagc
     6301 tttgcaacat cctgttcacc cgcgcgcttg ctcgccgtct gcaagggacg ggcgtgaccg
     6361 ccaattgcct ccatccgggc tttgtcgcca ctcgcttcgg cgatgaggcg ggcggcgccg
     6421 tcggcttgct catcccgctc gctaagctgt tcggccggcg cccagagaaa ggcgccgaga
     6481 cgatcgtcta tctcgcctca tcgcccgacg tcgcctcgga gagcggcttc tactatttcg
     6541 aatgccgcaa aacgaccccg tccgcggcgg cccaggatga cgaggcggcg cgccagctgt
     6601 ggacggcgag cgagaagctg gccggcttcg tctcctgagc ggcgaaaatc ggcggccggc
     6661 tcgccgctcg tttttcgcga tgagccgcgt caatacgacc tgccgtgtta acgcgatttt
     6721 tgcctatgcg gccatcgttt cgccgtccgc gttcatgtat ggtcgcaccg gtgaaatcac
     6781 cgcctgatcg cgttgtttgc gcggaagaaa ggccgtctcg gcttcaatga tctgggtcgt
     6841 tttcgctttg atgaccggcg cggccgttct ctgcgcgctt tggccgctgg cgcgcaagcg
     6901 cgcttcggcc gaaggcgaga cgcgcgccgt cgccttctac aaagcgcaaa tcgccgaaat
     6961 cgatcgcgac gtcgcgcgcg ggcaattgcc gcaagccgag gcggagggcg cgcggatcga
     7021 agcctctcgc cggctgatcg ccgcaagcga gcgcgccgag cgcggcggcg gcgcagcgcg
     7081 gccggctcct cgcctcgcgg cggcgctgct cattctgatc ggcgtgccgc tggtggcgtt
     7141 cggcctttac ggacgctacg gcaatcccgg cgagccggac gaaccgattg cggcgcggct
     7201 caacgacccc gcccatggcg acgatcttca ggcggcggtc gccaagatcg aatcgcatct
     7261 cctcacgcat cccaatgacg gcgtcggctt caaagtgatc gcgccggctt atttgcgcat
     7321 gggccgcttc gacgacgcgg tgaaggccta tggccaggcg ctgcgcctgc ttggcgatcg
     7381 tcccgagctt cgggccgatt acggcgaggc gctggtcgcc gccgcgaacg gcgtcgtcac
     7441 cgccgacgcc cgcgtcgcgt ttcagcaagc gctcgccaag gacccgcgat cggcgaaagc
     7501 gcgctactat ctcgccctcg ccgccgagca ggacggcgac aagcagcgcg ccggagagac
     7561 gtacaaggcg ctgctcgccg aatctccgaa cgcgccgtgg gccccgacgg taagaatgcg
     7621 ccttgccgcg cttggcgaaa tggcgccggc ggcgacggcg aaaatcgcgg cgccgccgaa
     7681 agaagaggcg gcggcgatcg cctccctgtc gccggagcaa aggcaggcgg cgattcacgg
     7741 catggtcgac cgtctcgccg cgcgcctcgc ggccaagggc gacgatccgg cgggctggct
     7801 gcgcctgatc cgcgcctatt cggtcttgca ggaacaggac aaggccaagg cggcgctcgc
     7861 caaagcgcgc gaggcgctga acggcgacgc cgccgccaag cgcgatctcg acgcgctcgc
     7921 caaagagctc ggactggaga gctgacgttg acgaggaagg gaaggcggct cactctaatc
     7981 ggcgtcgcgc tttgcgtgat cgccttcgcc gcaggccttg cgctttacgc gctgcgcgac
     8041 agcatcgtct tcttctacgg tccgagcgag ctcgccgaaa aggatgtgcg cccgggcgcg
     8101 catttgcgcg tcggcggcct cgtcaagccc ggctcgctgg tcaaagccgc ggatggcaag
     8161 atgtccttcg tgatcaccga caatgcgcat gacgtggcgg tgacctatca aggccaggtt
     8221 cccgatctct tccgcgaagg gcagggcgtc gtcgccgagg gcgttctcga agcgccggga
     8281 aaattccatg ccgacacgat cctcgccaag catgacgagc gctacatgcc gcgcgaagtg
     8341 gtcgacgctt tgaagaaaca ggggcgctgg caggaaggcg gaggcggatc gtgatcgtcg
     8401 aagccggcca ttacgcgctg acgctcgcct tggctttggc cttggtccag gccttcgcgc
     8461 cgctctgggg cgccgcgcat cgcgacgaag gcctgatcgg cgtcgctcag tcgaccgcga
     8521 tcggccaatt cgttgcgatc gcgttctcgt ttggcgcgct catcgaggcc catctggttt
     8581 cggatttctc ggtcctcaac gtcgccgaga actcccatac cgcgatcccg accctctaca
     8641 agatcaccgg cgtctgggga aaccacgagg gctcgatgct gctctgggtt ttcatccttg
     8701 ccctgttcgg cgcgctggtc gccgctttcg cccgcgccct gccgccggtg ctgcgcggca
     8761 atgtccttgc ggtgcagggc ctgatctccg ccgcctttct cgccttcatc ctgatcacgt
     8821 ccaatccgtt cgcgcggctc gatccggcgc cgttcgaagg ccgcgacttg aaccctgtct
     8881 tgcaggatct cggcctcgcc attcatccgc cgctcctcta tcttggctat gtcggcttct
     8941 cgatcgtctt ctccttcgcc gccgcagcct tgatcgaagg ccgggtcgat gcggcctggg
     9001 cgcgtttcgt ccggccgttc gcgctcatcg cctggagctt cctgacgctc ggcatcgcga
     9061 tgggctcgta ctgggcctac tacacgctgg gctggggcgg cttctggttc tgggatccgg
     9121 tcgagaacgc ttcgctcatg ccctggctcg ccggcaccgc tttcgttcac tcgatcgcgg
     9181 tgatggagaa gcgcgaagcg ctgaaagtct ggacgatttt cctcgccatc gtcgccttct
     9241 cgttttcgct gctcggcacc ttcctggtcc gctccggcgt cctgacctcg gtccacgctt
     9301 tcgccagcga tccgcagcgc ggcgttttca tcctgctcat cctcgccgcc tttatcggcg
     9361 gggccttggc tttgttcgcc tggcgcgcgc ctttgctcaa gcagggcggg ttgttcgctc
     9421 cggtttcgcg cgaaggcgcg ctggttttga acaacctgtt cctcaccacg agctgcgcgg
     9481 cggtgatgat cggcacgctc tatccgctcg ccctcgaaca gatgaccggc gagaaaatct
     9541 cggtcggcgc gcccttcttc aacatgacct gcggcgccct gctgatcgcg ctttgcgcga
     9601 tcgttccgtt cggccaatcg ctggcctgga agcgcggcga ccttctcggc gtggcgcagc
     9661 gcctcgccgt cgccggcgcg gcgggcctct gcatcgccgc cgcgcttctt acgctgacga
     9721 gcggcggtcc cgtcctggcg ccgcttggcg cgggcttggc ggtctacgtg atcctcggcg
     9781 ccttgagcga gatcgcggcg cggctttggc gcggcggcgc ctcggcgcgg atcgcgctcg
     9841 cccgcgcctg gggcctcccg ctctcggcct ggggcgccgc tttcgcgcat atcggcgtcg
     9901 gcgtgacttt gcttggcctc gccatgaccg gcttcggcgc cgaaacgatc gcgacgatga
     9961 aaatcggcga gccgcgcgcg gtcggtccct atctcgtcgt gttcgaatcg gcgcagccgc
    10021 ggctcgggcc gaactaccgc gagaccgtcg ccaaggccgc gatccgcaag gacggcgtcg
    10081 tcgtcgccac ggtcgagccg gcgaagcggt tcttcccggc gcgcgaaaca tcgacgaccg
    10141 aggcgggaat tgcgacgctc gatctcggtc aagtctatat ttcgatcggc gacgaaaccg
    10201 gcggcgccat cgcggcgcgg ctctattgga agccgctggt gacgctgatc tggatcggcg
    10261 cctgcatcat ggctttgggc ggcgctttgt cgctcgccga tcggcggctg cgcatcggcg
    10321 cgccggcccg ggccaagggc gcaatcgcgc cgcaagcggc gggggcgcag tagcgatgcg
    10381 ctctttcgta cttgctttcg cctttgcgac gttctgcgcg atcggcgtgg cgcaagccgt
    10441 gcagcccgat ga
//
LOCUS       DATGJW010000245         7229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1083878, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000245 DATGJW010000000
VERSION     DATGJW010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7229
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1083878"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(95..1324)
                     /locus_tag="VKU03_07610"
     CDS             complement(95..1324)
                     /locus_tag="VKU03_07610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016733111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HLJ71166.1"
                     /translation="MIDVNLDVNDQHENAMAWLTAMEALTALGVQRQTLYANVSRGRI
                     RAKPDPKDPRRSLYDGGDVKRLAGRGGGRRTVAAIAAETIGWGDPVLASSLSTVADGR
                     LIYRGRDAVELAKDATLEEVASLLWDAEARFEPAPPAAKGAPASPLHAALVALAERAA
                     VDPPSAGRSRAALAKEGGDLIGALAAAMLGPQAGADAALHRRMARAWRARRAEDGLRR
                     ALVLLADHELNASTFAARVAVSTGAPLAAGLIAGLAALSGPLHGGAVASVRALIAAAR
                     RATAEAAARDWLAQGRKLPAFGHPLYPNGDPRAAALLQRFAPRPLFEETRAAIEELTG
                     EAPNVDFALAAFADAYDLPAAAPFTIFAIARSVGWIAHAIEQALSGNLIRPRARYVGP
                     APILPREAGEADRPKLA"
     gene            1366..2454
                     /locus_tag="VKU03_07615"
     CDS             1366..2454
                     /locus_tag="VKU03_07615"
                     /EC_number="2.3.3.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007816570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate synthase"
                     /protein_id="HLJ71167.1"
                     /translation="MISGLDDVVAAETMLSDVDGAGGRLIIRGHSLDELAGSAEYEHI
                     LGLLWSGLFEDLPGEAGLAAALGRARVEIFERVAGAEALAARLPVVEAARALIAELAD
                     GDDLACALRLVAAPAAFAPALIRMQRGDAPIAPDPSLSHAADALRMLGRPAGAEEAAA
                     LNVYLVTVADHGLNASTFAARIVASTRAGLTSSALAAISALKGPLHGGAPGPVLDMLD
                     SIGTPEAAEAWLDAALDRGDRLMGFGHRVYRVRDPRADALKAAARRLSRAGSGAGGRL
                     ALAEAIEAAALRILARRKPNRPLKTNVEFYTALLLETLGFPREAFTAVFAMGRIGGWI
                     AHAREQALSGRLIRPQSRYIGPRPGEAA"
     gene            complement(2617..4506)
                     /locus_tag="VKU03_07620"
     CDS             complement(2617..4506)
                     /locus_tag="VKU03_07620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLJ71168.1"
                     /translation="MTLIDLTFARALSRRSALKTAAFGLAALAAPRGLYAAQAETETE
                     TYGLSSFGDLALPADFKHFDYVNPNAPKGGLLSLQITATAGNQNFDTFDTLNIFSKKG
                     DGAAGMTATFDTLMSATGDEPDSVYGLVARAVRISADKLAYRFLLRPEARFRDGSKVA
                     AADIAFSIETLMTKGHPTFSQILLDVESVAAEAEDVFLVNFVKERSRDAHLIVAGMPI
                     FSKAYWATRDFDASTTEPPPGSGPYKVSKFEQGRYIEFARDKNYWGKDLPVNIGLNNF
                     DRLRFEYFRDRDVAFQAFKAGVTNYHEEYTSRLWAVGYDFPAVREGRVKKEALHNGAP
                     TGSQGWYFNTRREQFKDPRVREALGLCFDFEWTNKNIMYSLYKRVVSYFQNSDMEAKG
                     KPGPDELKLLEPFRGKVPDEVFGDPYIPPVSDGSGSDRQLLRKANEMLLAAGCKRQGG
                     VLTLPSGKPFVIEFLDSSELLQPHTAPFEQNLRKLGIDAHSRIVDAAQYKARTENFDF
                     DVVTAAFGGNPTPGTELRVFFSSAAAKQVGSRNLAGVSDPVVDALVDKIAKAQSRAEL
                     NTACRVLDRVLRAAHYWVPMWYRDQALLAYWDAFSRPDKQPKLGTGAPDTWWWDEEKA
                     KKIGL"
     gene            4627..5613
                     /gene="nudC"
                     /locus_tag="VKU03_07625"
     CDS             4627..5613
                     /gene="nudC"
                     /locus_tag="VKU03_07625"
                     /EC_number="3.6.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000210 - NAD+ diphosphatase activity
                     [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) diphosphatase"
                     /protein_id="HLJ71169.1"
                     /translation="MNKALFRAPEPPRFGFAANSLDRLSERREDEAFLAAARSNPEAR
                     AVVFCRDMPVMKKAAGGFEPLLSLEEAGRLGRRRESVLLGAEADGAPVFAELLDDSAV
                     ELRADGSDGFLDKRLLVIPGRHDLSLLDMRSIAVQGLAPPALIGVMGQAKSILSWHAR
                     HRFCPNCGAPTRLAAAGWRRECDACKAQHFPRTDPVVIMLAVDGDNCLLGRQARFPKG
                     MYSCLAGFLEPGETIEDAVRREIREEAAIVCGAVSYLASQPWPFPSSLMIGCLAEAAT
                     REVRVDGVELEDARWFARDEARAMLDGRHPAGLAAPTAMAIAHHILRAWVEG"
     gene            complement(5628..5948)
                     /locus_tag="VKU03_07630"
     CDS             complement(5628..5948)
                     /locus_tag="VKU03_07630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020087420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux SMR transporter"
                     /protein_id="HLJ71170.1"
                     /translation="MAWIVLFAAGLLEVVWATAMKQSQGFVRPGPTAITLVAMLASVA
                     LLSAAMRTLPLGVAYMVWTGIGALGAFAIGVVVLGEAAGPLRLAAAALILAGLALMKL
                     SEGG"
     gene            complement(6133..6846)
                     /gene="rph"
                     /locus_tag="VKU03_07635"
     CDS             complement(6133..6846)
                     /gene="rph"
                     /locus_tag="VKU03_07635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004549 - tRNA-specific ribonuclease
                     activity [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease PH"
                     /protein_id="HLJ71171.1"
                     /translation="MRPSNRAPDELRKVSFERGVARYAEGSCLVRFGETHVLCTASLE
                     DKPPQWLRGQGRGWVTAEYSMLPRATATRTRRESAAGKVSGRTQEIQRLIGRSLRAAV
                     DLPRLGERQITLDCDVLQADGGTRTAAITGAWLALHDCLKWMTQRSILRDNPLREHVA
                     AISCGVCQGQAVIDLDYAEDSTAETDANFVMTGSGALIEVQGSAEGAPFSEEQLLALL
                     ALARGGVAKLVAMQKAALA"
     gene            complement(7088..>7229)
                     /locus_tag="VKU03_07640"
     CDS             complement(7088..>7229)
                     /locus_tag="VKU03_07640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013525174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ71172.1"
                     /translation="GRAGEPEEVAAVASFLASDDASYITGQTIYPDGGRLALNYTVPT
                     PD"
BASE COUNT         1121 a         2426 c         2537 g         1145 t
ORIGIN      
        1 cgggggaggg ggaccgcgcg aagcgcggtg gagggggcct cggcgccggg agataatcct
       61 gatccgccgc gccccctcca ccacccttcg ctcgctacgc gagcttcggg cggtccgcct
      121 cccccgcttc gcgggggagg atgggagccg gcccgacgta ccttgcgcgc ggacggatga
      181 gattgccgct caaggcctgc tcgatcgcat gagcgatcca gccgacggac cgcgcgatcg
      241 cgaagatcgt aaacggggcc gctgcgggga gatcgtacgc gtcggcgaaa gcggcgagag
      301 cgaagtcgac attcggcgct tcgccggtca actcctcgat tgccgcgcgc gtctcctcaa
      361 agagcggccg cggcgcaaag cgctgcagca gcgccgccgc gcgcggatcg ccgttcggat
      421 agagcgggtg gccgaaggcc ggcagcttgc gcccttgcgc cagccaatcg cgcgcggccg
      481 cctcggccgt ggcgcgcctg gcggcggcga tcagcgcccg gacggaggcg acggcgccgc
      541 catgcagcgg cccggacagc gccgccaggc cggcgatgag gccggcggcc agaggcgcgc
      601 cggttgagac ggcgacgcgc gcggcaaagg tcgatgcgtt gagttcgtga tcggcgagca
      661 ggacgagggc gcgccgcagc ccgtcttctg cgcggcgggc gcgccaggcg cgcgccatcc
      721 gccgatgcag cgccgcgtcc gcgccggctt gcggcccgag catggccgcg gcgagcgcgc
      781 cgattaagtc gccgccttct ttcgccagcg cggcgcgcga gcggccggcc gagggcgggt
      841 cgacggcggc gcgctcggcg agcgcgacca gagcggcgtg cagaggagag gcgggcgcgc
      901 ccttcgccgc gggcggcgca ggttcgaacc gcgcctcagc gtcccaaagc aggctcgcga
      961 cctcctccag cgtcgcgtct ttggcgagct cgaccgcgtc gcggccgcga tagatgaggc
     1021 gaccgtccgc gacggtcgag aggctcgacg ccagaaccgg atcgccccag ccgatcgtct
     1081 cggcggcgat tgccgcaacc gtgcgccggc cgccgccgcg gccggcgagg cgcttcacgt
     1141 cgccgccgtc atagaggctg cgccgcgggt ctttcggatc gggcttggcg cgaatgcggc
     1201 cgcggctgac attggcgtaa agggtttggc gctgcacgcc gagcgccgtc agagcctcca
     1261 tcgcggtcag ccaagccatg gcgttctcat gttgatcgtt cacatcaaga ttgacgtcaa
     1321 tcataaccga tccgatgttt aagcgaaacg ctatggagca aagtcatgat cagcggtctc
     1381 gacgacgtgg tggcggcgga gacgatgctt tccgatgtcg acggcgccgg cggacgcctg
     1441 atcatccgcg gccattcgct cgacgaactg gccgggagcg ccgagtacga gcatatcctc
     1501 ggacttctat ggtctggctt gtttgaagac ctgcccggcg aggccggcct cgccgccgcg
     1561 ctcggccgcg cccgcgtcga aattttcgag cgggtcgccg gcgcagaagc gctcgccgcc
     1621 cgcttgccgg tcgtcgaggc ggcgcgcgcc ctgatcgcgg aacttgccga tggcgacgat
     1681 ctcgcttgcg cgctgcgtct cgtcgcggcg ccggccgcgt tcgcgccggc gctcattcgc
     1741 atgcagcgcg gcgatgcgcc gatcgcgccc gatccgtctc taagccatgc cgccgacgcc
     1801 ctgcgcatgc tcggccggcc ggccggcgcc gaagaagcgg cggcgcttaa cgtttacctc
     1861 gtcacggttg ccgatcacgg cctcaacgcc tcgaccttcg cagcgcggat cgtcgcctcg
     1921 acgcgggccg gcctgacctc ctcggccctg gccgcgatca gcgcgttgaa agggccgttg
     1981 catggcggcg cgccgggacc ggtgctcgac atgctcgact cgatcggaac gccggaagcg
     2041 gccgaagctt ggctcgatgc ggcgctcgat cgcggcgacc ggctgatggg attcggccat
     2101 cgcgtctatc gtgtgcgcga tccgcgcgcc gacgcgctga aagccgcggc gcggcgcctc
     2161 tcgcgcgccg gctcaggggc cggcggacgg ctggctttgg ccgaggcgat cgaggcggcg
     2221 gcgctgcgca ttctcgcccg ccgcaagccg aaccggccgc tgaagaccaa tgtcgagttc
     2281 tatacggcgc tgctcttgga gacgctgggc tttccgcgcg aagcgttcac cgccgtcttc
     2341 gccatgggcc ggatcggcgg ctggatcgcc catgcgcgcg agcaggcgct gtcgggacgg
     2401 ctgatccggc cgcaatcgcg ctatatcggg ccgcgccccg gcgaggcggc gtgacgtact
     2461 catcctcccc cgtcgaagac gggggagggg gaccgcgcgt cagcgcggtg gagggggctc
     2521 caatctatca ggataatccc aaccggcgca gccccctcca ccgcgcttac gcgcggtccc
     2581 cctcccccgt cttcgacaag ggaggatcgt acgccgctaa agcccaattt tcttggcctt
     2641 ctcttcgtcc caccaccagg tgtcgggcgc gccggtgccg agcttgggct gcttgtcggg
     2701 ccgcgagaac gcgtcccaat aggcgagcaa agcctggtcg cggtaccaca tcggcaccca
     2761 gtaatgggcg gcgcgcagga cgcggtcgag aacccggcag gccgtgttga gctcggcgcg
     2821 cgactgcgcc ttggcgatct tgtcgacaag cgcgtcgacg accggatccg agacgccggc
     2881 cagattgcgc gagccgacct gcttggccgc ggcggaggag aagaagacgc gcaattcggt
     2941 tcccggcgtc ggattgccgc cgaacgcggc cgtgaccacg tcgaaatcga aattctcggt
     3001 gcgcgctttg tactgcgcgg cgtcgacgat gcgcgaatgc gcgtcgatgc cgagcttgcg
     3061 cagattctgc tcgaaaggcg cggtatgcgg ctgcagaagc tcggacgaat cgaggaattc
     3121 gatgacgaac ggcttgccgc tcggcagggt caacacgccg ccctggcgct tgcagccggc
     3181 ggcgagcagc atctcgttgg ccttgcgcag caattggcga tcgctgcccg atccgtccga
     3241 gaccggcggg atgtaagggt cgccgaagac ctcgtcgggg accttgccgc ggaacggctc
     3301 gagcaatttc agctcgtccg gcccgggctt gcctttcgcc tccatgtcgg aattctggaa
     3361 ataggacacc acgcgcttgt acagcgaata catgatgttc ttgttggtcc actcgaaatc
     3421 gaagcacagg ccgagcgcct cgcggacgcg cggatccttg aactgctcgc gcctcgtatt
     3481 gaaataccag ccttgcgagc cggtcggcgc gccgttatgg agcgcctcct tcttgacccg
     3541 cccttcgcgc acggcgggga aatcgtagcc gacggcccag agccgcgacg tatattcctc
     3601 gtgataattg gttacgcctg ccttgaaggc ctggaaggcg acgtcgcgat cgcggaaata
     3661 ttcgaagcgc agccggtcga aattgttcag gccgatattg accggcagat ccttgcccca
     3721 atagttcttg tcgcgcgcga actcgatata ccggccctgc tcgaacttgc tcactttgta
     3781 ggggccggag ccgggcggcg gctccgtcgt cgaggcgtcg aaatcccttg tcgcccaata
     3841 ggctttcgag aagatcggca tgccggcgac gatgagatgg gcgtcgcggc tgcgctcctt
     3901 gacgaagttg acgaggaaca cgtcctccgc ctcggccgcg acgctttcga cgtcgagaag
     3961 aatctgcgag aaggtcggat ggcccttggt catcaacgtc tcgatcgaga aggcgatgtc
     4021 ggccgcggcg actttcgagc cgtcgcgaaa gcgcgcctcc ggccgcagca gaaagcgata
     4081 ggcgagcttg tcggcggaaa tccgcaccgc gcgcgcgacg aggccgtaga cggaatcggg
     4141 ctcgtcgccg gtcgcgctca tcagcgtatc gaacgtcgcg gtcatgcccg cggcgccgtc
     4201 gcccttcttg gagaaaatat tgagcgtatc gaacgtatcg aaattctggt tgccggcggt
     4261 cgcggtgatc tgcagcgaca gcaggccgcc cttcggcgcg ttcggattga catagtcgaa
     4321 atgcttgaaa tccgccggca aagcgagatc gccgaaggac gaaaggccgt aggtttcggt
     4381 ttcggtttcg gcttgcgccg catagaggcc tcgcggcgcg gccagggcgg cgaggccgaa
     4441 ggcggcggtc ttcaacgccg agcggcggga cagcgcgcgc gcgaaggtca agtcgatgag
     4501 ggtcaaggcc gctcctgatt cgcttgcaaa ataatccgct tgcccggcgc ttgaccagcg
     4561 ccgccgccca tgcgcttttg ccgccggttt tgagcccctg gccgggttga acgaggaaat
     4621 aagggcatga acaaggcttt gttccgggcg ccggagcccc ctcgtttcgg cttcgcggcc
     4681 aattccttgg accggctgtc ggagcggcgc gaggacgaag cctttctcgc cgccgcccgc
     4741 tccaatcccg aggcgcgcgc cgtcgtcttt tgccgcgaca tgccggtgat gaaaaaggcc
     4801 gccggcggct ttgagccgct cctttcgctc gaagaggcgg ggaggctcgg ccggcgccgc
     4861 gaaagcgttc ttctcggcgc cgaggccgac ggcgcgccgg tcttcgccga gttgcttgac
     4921 gattccgccg tcgaattgcg agccgacggc agcgacggat ttctcgacaa gcgcctgctg
     4981 gtcattcccg ggcgccacga tttgagcttg ctcgacatgc gctcgatcgc cgtgcaaggc
     5041 ttggcgcccc ccgctctcat cggcgtcatg ggacaggcca aatcgatcct gtcctggcat
     5101 gcgcgccatc gcttctgccc gaattgcggc gcgccgaccc ggctcgccgc cgccggctgg
     5161 cggcgcgaat gcgacgcctg caaggcccag cactttccgc gcaccgaccc ggtcgtgatc
     5221 atgctggcgg tcgacggcga caattgcctg cttggccgcc aggcgcgctt tccgaagggc
     5281 atgtattcgt gccttgccgg ttttctcgag ccgggcgaga cgatcgagga cgcggtgagg
     5341 cgcgaaatcc gcgaggaggc ggcgatcgtt tgcggcgcgg tgtcctacct cgcctcgcaa
     5401 ccctggccgt ttccgagctc gctgatgatc ggctgcttgg ccgaggcggc gacgcgcgag
     5461 gtgcgcgtcg acggcgtcga gctggaggac gcgcggtggt tcgcgcgcga cgaggcgcgc
     5521 gccatgctgg acggccgcca tcccgccggg ctcgcggccc cgacggcgat ggcgatcgcc
     5581 catcatatcc tgcgcgcctg ggtggagggc tgagcgggcg ggcggcgtca gccgccctcg
     5641 gagagtttca tcagcgccag gccggcaagg atcagcgccg cggcggcgag ccgcagcggg
     5701 cctgcggcct cgccgagaac gacaacgccg atggcgaagg cgccgagcgc gccgattccg
     5761 gtccacacca tataggcgac gccgaggggc agggtccgca tcgcggcgga gaggagcgcg
     5821 acgctggcga gcatggcgac cagcgtgatc gcggtcgggc cagggcggac gaagccttgc
     5881 gactgtttca tcgcggtcgc ccaaacgact tccagcagac cggcggcgaa aagaacgatc
     5941 caggccatgc ggccctcctt tgagaaaggg ccgggccgtc ccggactttc gagcctcgat
     6001 ttgaggagga ggacgtggcc tcgccgaacg gagagatagg ggcgggccga acggcttgca
     6061 agcgccggcc cttgtagccg cggtcgctga ccgcgcttcc atgcccgccg accggcgatc
     6121 ggccgcgcgc tcttaagcga gcgccgcctt ctgcatggcg acgagcttgg cgacgccgcc
     6181 gcgcgccagc gcgagcaaag ccagcaattg ctcctcgctg aacggcgcgc cttcggccga
     6241 gccttgcact tcgatcaagg cgccgcttcc ggtcatgacg aaattggcgt cagtttccgc
     6301 ggtcgaatct tcggcgtagt cgagatcgat caccgcttgc ccctggcaaa cgccgcagga
     6361 gatcgccgcg acatgctcgc gtaaagggtt gtcgcggagg atcgagcgct gcgtcatcca
     6421 tttcaggcaa tcatgcagcg ccagccaggc gccggtgatc gccgccgtgc gggtgccgcc
     6481 gtcggcttgc agcacgtcgc aatcgagcgt gatctggcgc tcgccgagcc gcggcagatc
     6541 gaccgcggcg cgcagcgagc ggccgatcag gcgctggatt tcctgggtgc ggccggagac
     6601 cttgccggct gccgattcgc gccgcgtccg cgtcgcggtg gcgcgcggca gcatggaata
     6661 ttccgccgtc acccagccgc ggccctggcc gcgcagccat tgcggcggct tgtcctcgag
     6721 cgaggccgtg cacaggacat gcgtctcgcc gaagcgcacg aggcacgagc cctccgcata
     6781 gcgcgcgacg ccgcgctcga aactcacttt gcgcaattcg tccggcgcgc gattggaagg
     6841 gcgcatcgat gattccttaa ccagttgact ggcgggcgtg agcgcgacag ccgcacgtac
     6901 cctcatccgg cgcttcgcgc caccttctcc cgcaagcggg agaagggatt gtggccgcgg
     6961 tcagcgaccg caccccctct cccgcttgcg ggagagggtt ggggtgaggg taagccgcag
     7021 ccgtagccgt agcgaaaagg gaaacctgct tagcgctccg tcgggcgagg cgcaaccagg
     7081 gccccgctca gtccggcgtc ggcacggtgt agttcaaggc gagacggccg ccgtcgggat
     7141 agatggtctg accggtaatg taggaggcgt cgtcgctggc gaggaaagag gcgaccgcgg
     7201 cgacctcctc cggctcgcca gcccggccg
//
LOCUS       DATGJW010000246         3923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_669243, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000246 DATGJW010000000
VERSION     DATGJW010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3923
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669243"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VKU03_07645"
     CDS             complement(<1..560)
                     /locus_tag="VKU03_07645"
                     /EC_number="4.2.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxy-acid dehydratase"
                     /protein_id="HLJ71173.1"
                     /translation="MPAYRSRTSTHGRNMAGARGLWRATGMKDGDFGKPIIAIANSFT
                     QFVPGHVHLKDLGQLVAREVEAAGGVAKEFNTIAVDDGIAMGHDGMLYSLPSRELIAD
                     AVEFMVNAHCADALICISNCDKITPGMLMAALRLNIPAVFVSGGPMEAGKVVLAGKAK
                     KVDLIDAMVAAADSAVSESDLKVIERS"
     gene            complement(663..857)
                     /locus_tag="VKU03_07650"
     CDS             complement(663..857)
                     /locus_tag="VKU03_07650"
                     /inference="COORDINATES: protein motif:HMM:NF018404.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ClpX C4-type zinc finger protein"
                     /protein_id="HLJ71174.1"
                     /translation="MTQIECSFCGVSATKDDARDKYIAGPNVFICRDCVDTMIEIFCE
                     TDPEWGRRTMKRLAERAKGE"
     gene            complement(1251..2879)
                     /locus_tag="VKU03_07655"
     CDS             complement(1251..2879)
                     /locus_tag="VKU03_07655"
                     /EC_number="5.4.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016868 - intramolecular
                     phosphotransferase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-D-glucose phosphate-specific
                     phosphoglucomutase"
                     /protein_id="HLJ71175.1"
                     /translation="MILTIDTKPFDDQKPGTSGLRKKVAHFQQPHYVENFIQSIFDSL
                     RGYAGQMLVVGGDGRYYNREAIQKVIKIAAANGFGRLLVGRGGLISTPAVSCIIRGRQ
                     AFGGIILSASHNPGGPDGDFGVKYNIGAGGPAPEKVTDAIFACSKAIARYRISDAPNI
                     ELDRIGVTRVENTEVEIIDPVADYKALMKTLFDFEAIEALFDSGFKMRFDAMHAVTGP
                     YAHAILEDELGATRGTVVNGTPLPDFGGQHPDPNLTYAKDLYDLMMSPEAPNFGAASD
                     GDGDRNLIIGRGIFITPSDSLAMLAANATQAPGYAKGLAGIARSMPTSAAADRVAAHL
                     NIKCYETPTGWKFFGNLLDAGLATLCGEESSGAGSNHVREKDGLWAVLLWLNILAKRR
                     ISVADLAREHWATYGRNYYTRHDYEELDLSAANGLMDALRGQFATLPGKTFGPHKVAA
                     ADDFAYHDPVDGSDTEKQGIRVMFEGGSRIVYRLSGTGTVGATLRVYIERYEPPTGNL
                     GQDAQAALADFIALSRSLAEIEKRTGRTAPSVIT"
     gene            complement(3160..3822)
                     /locus_tag="VKU03_07660"
     CDS             complement(3160..3822)
                     /locus_tag="VKU03_07660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLJ71176.1"
                     /translation="MSERTHILIADDHPLILGALRHALIGAIEGVEIHEARDLETLTA
                     ALEARPDMDLVLLDLAMPGVRGFSGLIYLRAQHAGAPVVVVSANRDPAVMRACIELGA
                     AGYIPKSLDLETMRAAVRAVLEGGRWIPPELDLAAPADQNSSLALRRLSSLTPQQVRV
                     LMMLSEGLLNKQIAYELSVSEATVKAHVSAILQKLGVESRTQAVIAAANIEKGLWPAK
                     PE"
BASE COUNT          670 a         1251 c         1287 g          715 t
ORIGIN      
        1 gagcgctcga tgactttcag gtcgctctcg ctgaccgcgc tgtcggctgc cgcgaccatc
       61 gcgtcgatga ggtcgacctt cttcgccttg ccggcgagca cgaccttgcc cgcctccatc
      121 ggcccgcccg aaacgaaaac cgccggaata ttgaggcgaa gcgccgccat cagcatgccg
      181 ggcgtgatct tgtcgcaatt ggagatgcag atcagcgcgt cggcgcaatg cgcattgacc
      241 atgaattcga cggcgtcagc gatcaattcg cgcgacggca gcgaatagag catgccgtca
      301 tggcccatcg cgatgccgtc gtcgacggcg atcgtgttga actccttggc gacgccgccg
      361 gccgcctcga cctcgcgcgc gacgagttgg ccgagatcct tcagatggac gtggccgggc
      421 acgaattgcg tgaacgaatt ggcgatggcg atgatcggct tgccgaaatc gccgtccttc
      481 atgccggtgg cgcgccagag gccgcgcgcg cccgccatgt tgcggccgtg agtggaagtg
      541 cgcgagcggt aagcgggcat ggacgtaaac ctggtgagag cggtccggcg gcgatatcgc
      601 gggccgggac ctctgcttgt cgcaaaaagc ggccaaggcc gcaatgaaat ccggcggagg
      661 tcttattcgc ctttcgcccg ctccgccagt cgcttcatcg tccgccgtcc ccattccggg
      721 tccgtctcgc agaagatttc aatcatcgtg tccacgcaat cgcggcagat gaacacgttt
      781 ggcccggcaa tatatttgtc tcgggcgtca tctttcgtcg ccgagacgcc gcagaacgag
      841 cattcaattt gcgtcatttc ccatcgccca ttcttaagat cgcgtctgcc tttcgcggcg
      901 ttcccggccg ccgccctgcg aaggaaaaat aaaaatttcc gttcggaagg gcttttcctc
      961 ctttcggaag cctttttgga aggaaaagcc ttccgcgcgc cccgctaacc ctttgatctg
     1021 actaggcggg aaataacgcg atttcccccg accaaaggat atcaagcatt tgagccggtt
     1081 ttctttcctt cgcgcttttt gcgttggagc agaacgcggt cttgcaggtg ctgacttggg
     1141 tcagcatacg ccaatttctg aaattctgaa agaaaaattt cgcgcgtcgg gcggctccgc
     1201 gctccgtcgc gagccgcgcc gctcggagac ttcagtcgat cgggtagcgg tcaggtgatc
     1261 acgctcggcg ccgtgcgccc ggtgcgcttc tcgatctcgg cgagcgagcg cgacagggcg
     1321 atgaaatcgg ccagcgcggc ttgcgcgtcc tggccgagat tgccggtcgg cggctcgtag
     1381 cgctcgatat agacgcgcag cgtcgcgccg accgtgccgg tgccggagag gcggtagacg
     1441 atgcgcgagc cgccttcgaa catcacgcgt atcccctgct tttcggtatc ggagccgtca
     1501 accggatcgt gataggcgaa atcgtcggct gcggcgactt tgtgcggccc gaaggtcttt
     1561 cccggcagcg tcgcgaactg gccgcgcagc gcgtccatca ggccgttggc ggcggaaaga
     1621 tcgagctcct cgtaatcgtg gcgggtgtag tagttgcgcc cgtacgtcgc ccagtgctcg
     1681 cgggcgagat cggcgaccga gatgcggcgc ttggcgagga tgttgagcca gagcaacacc
     1741 gcccacagcc cgtccttctc gcgcacatga ttggagccgg cgccggagct ttcctcgccg
     1801 cacaatgtgg cgaggccggc gtcgagcagg ttgccgaaga acttccagcc tgtcggcgtc
     1861 tcatagcatt tgatgttgag atgagcggcg acgcggtcgg cggcggcgct ggtcggcatc
     1921 gagcgggcga ttccggcaag gcctttcgcg tagcccggcg cctgcgtcgc attggccgcg
     1981 agcatggcga gcgaatcgga cggggtgatg aaaatgccgc ggccgatgat caggttgcga
     2041 tcgccgtcgc cgtccgacgc ggcgccgaaa ttcggcgcct ccggcgacat catgagatcg
     2101 tagagatcct tggcgtaggt gaggttcggg tcgggatgtt ggccgccgaa atcgggcagc
     2161 ggcgtcccgt tgaccacggt gccccgcgtc gcgccgagct cgtcttcgag aatggcgtgg
     2221 gcgtaagggc cggtcacggc atgcatcgca tcgaagcgca tcttgaagcc ggaatcgaac
     2281 aaggcttcga tcgcctcgaa atcgaacaag gtcttcatca aggccttgta gtcggcgacc
     2341 ggatcgatga tctcgacctc cgtgttctcg acgcgggtga cgccgattct gtcgagctcg
     2401 atattcggcg cgtcggaaat ccggtagcgc gcgatcgcct tgctgcacgc gaaaatcgcg
     2461 tcggtgacct tttccggggc cgggccgccc gccccgatat tgtacttgac gccgaaatcg
     2521 ccgtccggcc cgccgggatt gtggctcgcc gacaggatga tgccgccgaa cgcctggcgg
     2581 ccgcggatga tgcaggagac ggccggcgtc gagatcagcc cgccgcggcc gaccagcagc
     2641 cggccgaagc cgttggcggc ggcgatcttg atcaccttct ggatcgcctc gcgattgtag
     2701 tagcgcccat cgccgccgac gaccagcatc tggccggcat agcctcggag cgaatcgaaa
     2761 atcgactgga tgaaattctc gacgtaatgc ggctgctgga aatgcgccac cttcttgcgc
     2821 aggcccgacg tgcccggctt ctgatcgtcg aagggcttgg tgtcgatcgt gaggatcatg
     2881 ccgcggcgct cccacccctg aaacgggccg gctttggcgc cagccctggc tggaagacta
     2941 gcgcgggatc attcccgcgc aagccggaaa cgcgcattcc cctcgcctcg cgcagcgggg
     3001 agggcggcgg cgaagtcgcc gggcgagggg cggcgcagag cttaccgatt agtcttcgaa
     3061 aggcgcgtac cttctgagtc cggcgccgcc cctcatccgg cgcttcgcgt cgccttgtcg
     3121 ccgccttgcg gggagctatg actgcgcgcc gccgccagct cactccggct tggccggcca
     3181 caggcccttc tcgatattcg ccgcggcgat caccgcttgc gtgcggctct cgacgccgag
     3241 cttttgcagg atcgccgaaa catgcgcctt caccgtcgct tccgagaccg agagctcgta
     3301 ggcgatctgc ttgttgagca ggccttccga cagcatcatg aggacgcgca cttgctgcgg
     3361 ggtgagcgag gagagccggc gcaaggcgag gctcgaattc tggtcggcgg gcgctgcgag
     3421 atcgagctcg ggcggaatcc agcgcccgcc ttcgagaacc gcgcggaccg cggcgcgcat
     3481 cgtctcgagg tcgagcgatt tcgggatata gccggccgcg ccgagctcga tgcaggcgcg
     3541 catcacggcg ggatcgcggt tggccgagac gacgacgacc ggcgcgccgg catgctgggc
     3601 gcgcaggtag atgaggccgg aaaaaccgcg cacccccggc atcgccagat cgagcaggac
     3661 gaggtccata tccggccgcg cctcgagcgc cgcggtgagc gtctcgagat cacgcgcctc
     3721 gtgaatctcc accccctcga tcgcgccgat caaggcgtgg cgcaaggcgc cgaggatcag
     3781 cggatgatcg tcggcgatca ggatatgcgt tctctcgctc aacagggatc tccaggcgcc
     3841 gcctaatcgg cgacaatctc ttaccatact gcaaagcgct ctcgacgcat ccccgcgcgg
     3901 gcctgaaatg gacacggcac cgg
//
LOCUS       DATGJW010000247         8491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_669278, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000247 DATGJW010000000
VERSION     DATGJW010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8491
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_669278"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..839)
                     /locus_tag="VKU03_07665"
     CDS             complement(156..839)
                     /locus_tag="VKU03_07665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter family protein"
                     /protein_id="HLJ71177.1"
                     /translation="MIQDAVDAFAQIFTPPFRAVLIKSLALTLAILIIAWFGLDRLLV
                     SFVGFGPSWLSTILAILAGLGLFVGLAFLVAPITSLTAGFFLDEIALVVEREINPSGP
                     LGQPVAFGAAALCALRFAGLSILVNLVALALLLAPGVNVFAFLAANGYLLGREYFELA
                     AMRYRSFEEARAMRRHFGVQVFAAGLIVAGFVAVPVLNLCTPLFATAFMTRLHRRWTL
                     ERTSSGQPR"
     gene            complement(836..1990)
                     /locus_tag="VKU03_07670"
     CDS             complement(836..1990)
                     /locus_tag="VKU03_07670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015685148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLJ71178.1"
                     /translation="MHVVGNPKLSVIAPTYREAANVPVLFERLKKVLGGLDWELIVVD
                     DDSPDGTSEVAFALAAEDRRIRCIRRVNRLGLAGAVIEGVLSSSADFVAVIDADLQHD
                     ESILPRLYQGLVKGADLAVGVRVAQDGAERGLSPARQALSDAGARLFKRISGLSVSDP
                     MSGFFMIRRDIVAAIAPRLSPDGFKILADILLCAPPGIRIAEIPYVFRKRQAGQSKLS
                     AVVGLDFLGLIAHHATAGILPIRFALFAAVGASGLVVHLAVLKILVGLYGTTSFGAEQ
                     LVATLAAMTSNFLINNEVTYRENRYRGWRKLVGLCAFALVCSVGAVANVNIAAWLFRR
                     QQVWWVAGFSGALVGVVWNYAVSSAFVWRKRRAGAPRVVASPSSPPTLAS"
     gene            complement(2002..3585)
                     /locus_tag="VKU03_07675"
     CDS             complement(2002..3585)
                     /locus_tag="VKU03_07675"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLJ71179.1"
                     /translation="MQQSPRATMVLDGAAPAGRIAAPASAVALAIILGFALARVIFAV
                     SLDFGIDEAYTLAIARRLDWSYFDHPPLHQWIAHFAALAFGENAAARLPFIALFAGTG
                     WLVFALTRDLFGAKAGLWAVFALNATPFFFASAGAWIVPDGPLLCALASAAWSLSRLF
                     FHKAAAIWRLWLAAGVSLGLAGLSKYNAAFFPLGLALFLGFSREHRRWLGHPAPYCAA
                     LVAIVLIAPVLVWNAENGWVSFLFQGGRGAPGGHWRPAQVAAMALSEIALLSPWIFLP
                     LLAGVIGAVRRVLAGRDDGGRRLLLLCLALPVIVVFTLTPLWGARGLPHWPMPGWLFA
                     YPLLGAWLAEREGLLARRAWAFGSAAALALVAAIAVAEARTGFLVSFVPRQGGGADPT
                     LEALSWAKLRQSPLFANARPAFVVATKWTEAGKIAQALGPHVPVAVFSNEPHGFAFLH
                     DPASFVGEDAVIVVARKRLAESLAALAPYFAGLGEPQSLSLGRGGRDEIDLALVPARR
                     LLRPYPLPYPRRADSAPQS"
     gene            3733..4155
                     /locus_tag="VKU03_07680"
     CDS             3733..4155
                     /locus_tag="VKU03_07680"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71180.1"
                     /translation="MLPFILAAAISTSSIPSLDISAGCRPRSSIEDQALDYKSCMRQE
                     NETKQKLLKEWGSYPAAARRQCVSDRSSLVNSYVELLTCIEMQSWKNQPAMNPSGAAA
                     GGGAKTGGAVGGSPPLPEQMRGSSISHPLGGRPGAHLP"
     gene            complement(4169..4636)
                     /locus_tag="VKU03_07685"
     CDS             complement(4169..4636)
                     /locus_tag="VKU03_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012115247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLJ71181.1"
                     /translation="MSAFHSRVSMEDFASRAPAVPAALRRLGEAIDASGLDKALTELV
                     KLRASQINGCAYCVQFHLNLLRKLGVASEKLDLIAVWREAGIFSEREAAALAWTEALT
                     EAPRQDAGDEAYAALLTQFSEQEALFLTAAIGLINAWNRIGVGLRFAPPIPGR"
     gene            complement(4683..5651)
                     /locus_tag="VKU03_07690"
     CDS             complement(4683..5651)
                     /locus_tag="VKU03_07690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ71182.1"
                     /translation="MKLIATPDNPIPPGAILSAARAIDGAPLRVARWVPPTARRGTVA
                     ILPGRAEFIEKYFETVGELIARGFAVAALDWRGQGGSPRQLRNPRKGHVDDFSLYERD
                     LSALVDDVLGPSCPQPWFGLAHSMGAAVMLAVAHAGRSPFERLVLSSPMISLANQRYP
                     CLLRVAAEGLDTLGFGGAFAPGGGAAAVSAKPFADNVLTSDPKRYARTAAIIAAAPDL
                     AIGWPTIGWLHAAIRAMREFNDPDFAREIATPILIVAAGRDRVVDTRAAERFASHLRT
                     GRLIVIDGAEHEILVERDSYRELFWTAFDQFIPGIEGERTHARISA"
     gene            complement(5739..6704)
                     /locus_tag="VKU03_07695"
     CDS             complement(5739..6704)
                     /locus_tag="VKU03_07695"
                     /EC_number="1.13.12.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006470561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase family protein"
                     /protein_id="HLJ71183.1"
                     /translation="MPLPPILRDKLRLPVIGAPLFIVSNPDLVIAQCTAGIVGSFPAL
                     NARPASLLDEWLHRITEELAAWDRDHPDQPAAPFAVNQIVHKTNARLEQDVELTVKWR
                     APIVITSLGAREDVNVAVHAYGGITLHDVISDRFARKAIEKGADGLIAVAAGAGGHAG
                     AMSPFALIQDIRAWFNGPLALSGAIARGAAILAAQVMGADLAYIGSAFIATREANAAE
                     GHKQAIVRGRGEDILGTKYFTGIFGNYLRPSIVAAGLDPDNLPQGDPERMVEITSGGS
                     KPKAWRDIWGSGQGIGPVDQVTSAGELVARLAREYAEAKAAVAAA"
     gene            6907..7221
                     /locus_tag="VKU03_07700"
     CDS             6907..7221
                     /locus_tag="VKU03_07700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71184.1"
                     /translation="MRFSRLALVLLLSFPTDLRAADISFSCRVHHPPHLEIVGTNRGP
                     PKYCRWVCWFKLLDNPYIGFVKSNGYEKLSGTKVWTRTGDDSIDWTTINWRCRARPPA
                     TS"
     gene            complement(7340..8140)
                     /locus_tag="VKU03_07705"
     CDS             complement(7340..8140)
                     /locus_tag="VKU03_07705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71185.1"
                     /translation="MSADLSDVDFFTAHAAALFAKALPEAKVEVKGALALSIALPGRG
                     AESLWLNNVYSFCLRNPEACEEGLVSHVASMAASFKAPDKIERSQLRAIVRPAAMVEA
                     ADKAYARRGGASVSERLIGGLWVMCAQDQPGAVAILNANELATLKLSREQALAACKQN
                     VAAALPPLEPFRRDYPWPGVNLVAGDPYVTSWLIFPERWTAIAESVDGDLLVAAPAND
                     ALIYANGRDNDAVAALAEAAAFVAARAQKPLSTAVLRWTPTGWEEAKP"
BASE COUNT         1615 a         2747 c         2888 g         1241 t
ORIGIN      
        1 tttcatgaat atttccgatc gaagtctttt tactaccgcc agggacggga aagaggtcaa
       61 ttgcgaatta agtgcagata cctggatgat cctgaacgaa atttgcagtc aaataaaaca
      121 agcctttgac tgacgttcaa atacacggca ccgctttatc gtggctgccc cgacgaggtt
      181 cgttccagag tccatcgccg gtgcaatcgc gtcatgaacg cggtcgcaaa gagcggcgtg
      241 cataaattca gcaccggcac ggcgacgaag cctgcgacga tcaggccggc ggcgaagact
      301 tgaacgccga aatgacggcg catcgcccgc gcttcttcga acgatcggta gcgcatcgcc
      361 gcgagctcga aatattcgcg tcccagcaga tagccgttgg cggcgagaaa ggcgaagacg
      421 ttgacgcccg gcgcgagcag cagcgccagc gcgaccaggt tgacgaggat cgacaggccg
      481 gcgaagcgca gcgcgcaaag cgcggcggcg ccgaaggcga cgggctggcc gagcggaccg
      541 gacggattga tctcgcgttc gacgacgaga gcgatctcgt cgagaaagaa gccggccgtc
      601 agcgacgtga tcggcgcgac aagaaaggcg aggccgacga agaggccgag gccggcgagg
      661 atcgcgagga tcgtcgacag ccaggacggg ccgaagccga caaacgagac gagcagccgg
      721 tcgagtccga accaggcgat gatcagaatc gccagcgtca gcgccagcga tttgatcagg
      781 acggcgcgaa acggcggcgt gaagatttgc gcgaacgcgt cgacggcgtc ttgaatcatg
      841 aggcgagcgt cggcggcgaa gacggcgagg ccacgacgcg cggcgcgccg gcgcgtcgct
      901 tgcgccagac gaaggcggag ctgacggcgt agttccagac gacgccgacc agcgcgccgg
      961 aaaagccggc gacccaccag acttgctggc gccggaaaag ccaggcggcg atgttgacat
     1021 tggcgaccgc gccgaccgag cagacgagcg cgaaggcgca taggccgacc aatttgcgcc
     1081 agccgcgata gcggttctcg cggtaagtga cctcgttgtt gatcaggaaa ttgctggtca
     1141 tcgccgccaa tgtggcgacg agctgctcgg cgccgaaact cgtcgtgccg tagaggccga
     1201 ccaagatttt caatacggcg agatgcacga cgaggcccga cgcgccgacc gccgcaaaca
     1261 gcgcgaaacg gatcggcaga atgccggccg tcgcgtgatg ggcgatgagg ccgagaaaat
     1321 cgaggccgac aacggccgaa agcttcgatt gcccggcttg gcgcttgcgg aacacgtaag
     1381 gaatttcagc gatgcggatt cccggcggcg cgcagaggag gatgtcggcg aggattttga
     1441 agccgtcggg cgataagcgc ggcgcgatcg ccgcgacgat gtcgcggcgg atcatgaaga
     1501 agccgctcat cggatcgctg accgagagtc ccgatatgcg tttgaagagc cgcgcgccgg
     1561 cgtcgctcag ggcctgccgc gccggcgaga ggccgcgttc ggcgccatct tgggcgacgc
     1621 gcacgccgac ggcgagatcg gcgcctttga cgaggccctg gtacaatcgc ggcagaatgg
     1681 attcgtcatg ctgcaaatcg gcgtcgatga cggcgacgaa atcggcgctc gatgagagga
     1741 cgccctcgat caccgcgccg gccaggccga gccggttgac gcggcggatg cagcggatgc
     1801 gccggtcctc ggcggccagg gcgaaggcga cctccgacgt gccgtcgggc gaatcgtcgt
     1861 cgacgacgat caattcccaa tcgagtccgc ccagcacctt tttgaggcgc tcgaacagca
     1921 ccggcacatt ggcggcttcg cggtaggtcg gcgcgatcac cgagagcttg ggatttccaa
     1981 cgacgtgcat agcgcgcctt ctcaagattg cggcgccgaa tcggcgcgcc gaggataggg
     2041 cagcggatag ggccgcaaca ggcgccgcgc cggaaccaga gcgagatcga tttcgtcgcg
     2101 gccgccgcgg ccaaggctca aagattgagg ctcgccgagc ccggcgaaat agggcgcaag
     2161 ggcggcgagc gattcggcca agcgcttccg ggcgacgacg atgaccgcgt cctcgccgac
     2221 gaaagaggct ggatcatgca gaaaagcaaa gccgtgcggc tcgttggaga agaccgcaac
     2281 cgggacatgg gggccgagcg cctgggcgat tttgccggct tccgtccatt tcgtcgcgac
     2341 gacgaaggcc ggccgggcgt tggcgaatag cggcgattgg cgcagcttcg cccagctcaa
     2401 ggcctccagc gtcggatcgg cgccgccgcc ttgtctcggt acgaaagaaa ccaggaatcc
     2461 ggtccgcgct tccgcgacgg cgatcgcggc gaccagcgcg agcgccgccg ccgagccaaa
     2521 agcccaggcg cgccgagcca gcaagccctc gcgctcggcg agccacgcgc cgagcagcgg
     2581 ataggcgaag agccagcccg gcatcggcca atgcggcagg ccgcgcgcgc cccaaagcgg
     2641 cgtcagcgtg aagacgacaa tgaccggcag cgcgaggcaa agaagcaaga gccggcgccc
     2701 gccatcgtct cttcccgcca aaacgcgacg aaccgccccg atcacgccgg caaggagcgg
     2761 caggaaaatc cacggcgaga ggagcgcgat ctcgctcagc gccatcgccg cgacttgcgc
     2821 tggccgccaa tggccgcccg gcgcgccgcg cccgccctgg aagaggaacg acacccagcc
     2881 gttctccgcg ttccagacga ggaccggcgc gatcaagacg attgcgacga gcgcggcgca
     2941 ataaggcgcc gggtggccga gccagcggcg atgctcgcgc gaaaagccaa ggaaaagcgc
     3001 aaggcccagg ggaaagaagg cggcgttgta tttcgaaagg ccggccaggc cgaggctcac
     3061 gcccgccgcc agccagagcc gccaaattgc ggcagctttg tggaagaaga ggcggctcaa
     3121 ggaccaagcg gcggaggcga gcgcgcagag gagcggcccg tcgggcacga tccacgcccc
     3181 ggccgaggcg aagaagaacg gcgtcgcgtt cagcgcgaaa accgcccaga ggccggcttt
     3241 cgcgccgaaa aggtcgcgcg tcaaggcaaa gaccagccag ccggttccgg cgaagagcgc
     3301 gatgaacggc agccgcgcgg cggcgttttc gccaaacgcc agagcggcga aatgcgcgat
     3361 ccactggtgc agcggcgggt gatcgaaata ggaccagtcg aggcggcgcg cgatcgccag
     3421 ggtataggct tcgtcgatgc cgaaatcgag cgaaacggcg aaaatgaccc gcgccagcgc
     3481 gaagccgaga atgatcgcga gcgccacggc gcttgctggc gcggcgatcc gccccgccgg
     3541 cgccgcgccg tccaaaacca tcgtcgcccg cggcgattgc tgcaaatctt gccccgttca
     3601 gccggccgcc gtccggccgc agcctgcaat ggggtcagcg gcgcgtcctg tcaatcagcc
     3661 ggggtcggag gcggaagcca gaacatttta agggcgcgcc gcgttgagga agcgccgcgc
     3721 acggagggcg ccatgctgcc gttcattctt gccgccgcga tctcgacaag ctccatcccg
     3781 tcgctggaca tttcggccgg ctgccggccg cggagttcga tcgaagatca agcgctggac
     3841 tacaaaagtt gcatgcgcca ggagaacgag accaagcaga aactcctcaa ggaatggggc
     3901 tcctatcccg ccgcggcgcg ccggcaatgc gtctccgacc ggtcgagcct cgtcaacagc
     3961 tatgtcgagc ttttgacctg catcgagatg caaagctgga agaaccagcc ggcgatgaat
     4021 ccatccggcg ccgcggcagg gggcggcgca aaaaccggcg gggccgtcgg cggatcgccg
     4081 ccgctgccgg aacaaatgcg cggctcgtcg atcagccatc cgcttggcgg gagacccggc
     4141 gcgcatctcc cgtagaagag cgcgccaatc agcgccccgg aatcggcggc gcgaagcgca
     4201 ggccgacgcc tatgcgattc catgcattga tcaatccgat ggcggcggtg aggaagagcg
     4261 cctcttgctc gctgaactgg gtgagaagcg ccgcgtaagc ctcgtcgccc gcgtcctgcc
     4321 ttggcgcttc ggtcagcgcc tcggtccaag cgagcgcggc ggcttctcgc tccgagaaaa
     4381 tccctgcctc gcgccagacc gcgatgaggt cgagcttttc cgacgcgacg ccgagcttgc
     4441 gcaaaagatt gagatggaat tgcacgcaat aggcgcagcc attgatctgc gaagccctaa
     4501 gcttgacgag ttcggttagc gccttgtcga gcccggacgc gtcgatcgct tcgccgagcc
     4561 ggcgcaaggc cgccggcacg gcgggggcgc ggctcgcgaa atcctccatg ctgacccgcg
     4621 aatggaatgc gctcatcggg cttctccttt tccatgtttg acgggacgag gcgcggcttc
     4681 ggtcacgccg atatgcgggc gtgcgtccgc tcgccttcga ttccgggaat gaattggtcg
     4741 aacgccgtcc aaaagagctc gcggtaggaa tcgcgttcga ccaggatctc gtgctcggcg
     4801 ccgtcgatca cgatcagccg gccggtccgc aaatgcgagg cgaagcgctc ggcggcgcgc
     4861 gtatcgacga cccggtcgcg gcccgccgcg acgatcagga tgggcgttgc gatctcgcgc
     4921 gcgaaatccg gatcgttaaa ttcgcgcatg gcgcgaattg cggcgtggag ccagccgatc
     4981 gtcggccagc caatggcgag gtccggcgcc gcggcgatga tcgccgctgt gcgggcgtag
     5041 cgcttcggat cggaggtcag gacattgtcc gcaaagggct tggccgacac ggccgccgcg
     5101 ccgccgcccg gcgcgaaagc gccgccaaag ccgagcgtat ccaagccttc cgccgcgacg
     5161 cgcaggaggc agggatagcg ctgattggcg aggctgatca tcggcgaaga gaggacaagc
     5221 cgctcaaacg ggctgcggcc ggcatgagcg acggcgagca tgaccgccgc gcccatcgaa
     5281 tgggcgaggc cgaaccaggg ctgcgggcag gaggggccga gaacgtcgtc gaccagcgcg
     5341 gaaagatcgc gctcatagag cgagaaatcg tcgacatggc ctttgcgcgg attgcggagc
     5401 tggcgcggcg agccgccctg gccgcgccag tcgagcgcgg cgacggcgaa gccccgcgcg
     5461 atcaattcgc cgaccgtttc gaaatatttc tcgatgaatt ccgcgcggcc cggcagaatg
     5521 gcgaccgtac cgcggcgcgc cgtcggcgga acccagcgcg cgacgcgcaa cggcgcgccg
     5581 tcgatcgccc gcgcggccga gagaatcgcg ccgggcggaa tcggattgtc gggcgtggcg
     5641 atgagcttca tgggtccaga atttgtcgca tgccgcgcct gccggcgcaa cgcagccaga
     5701 cggcgagcga cagtacggcg aatgaatctg cggctcgctc acgccgccgc cactgccgcc
     5761 ttggcttcgg cgtattcgcg ggcgaggcgc gcgacaagtt cgccggcgct cgtcacttga
     5821 tcgacggggc cgatcccttg gccgctgccc caaatatcgc gccacgcctt cggcttcgag
     5881 ccgccgctgg tgatttcaac cattctctcg ggatcgccct gcggcagatt gtcgggatcg
     5941 agcccggccg ccacgatcga tgggcgcaga taattgccga aaatgccggt gaagtatttc
     6001 gtgccgagaa tgtcttcgcc gcggccgcgc acgatcgcct gcttgtggcc ctcggcggcg
     6061 ttggcttcgc gcgtcgcgat gaaggctgag ccgatatagg cgaggtccgc gcccatgacc
     6121 tgcgcggcga gaatggcggc gccgcgcgcg atcgcgcccg acaaggcgag cgggccgttg
     6181 aaccaggcgc gaatatcctg aataagcgcg aaaggcgaca tcgcgccggc atggccgccg
     6241 gcgcccgccg ccaccgcgat cagcccgtcg gcgcctttct cgatcgcctt gcgcgcgaag
     6301 cggtcgctga tcacgtcgtg cagcgtgatt ccgccatagg cgtggacggc gacgttcacg
     6361 tcctcgcgcg cgccgagcga ggtgatgacg atcggcgccc gccatttgac cgtcaactcg
     6421 acatcctgct cgagccgcgc attggtcttg tgcacgatct ggttgaccgc gaagggcgcg
     6481 gcgggctgat cgggatgatc gcggtcccag gccgccaatt cctcggtgat gcgatgcagc
     6541 cattcgtcga gcagcgacgc cggccgcgca ttcagcgctg gaaacgagcc cacgatcccc
     6601 gccgtgcact gcgcaatgac cagatcgggg ttggagacga tgaagagcgg cgcgccgatg
     6661 accggcaggc gcagcttgtc gcgcagaatg ggcgggagag gcatgccggc gagtcctcgg
     6721 atttggttta tgtataaacc aagcccgggc gcggcggcaa ttcgaggcgc cgtgtcatgt
     6781 ccgcgcaaac cggcgcgcca aaccgttcgc gccctgcggc tctccggacc agggaagggt
     6841 tatcgccaac ggcgccgttc ggccgcgtca tgcgtttctt ctcatgcgcc cgaaacgctg
     6901 gacgtcatgc gattctcccg actggccctc gtcctcctac tttccttccc gaccgatctg
     6961 cgcgcggccg atatcagctt ctcgtgccgc gttcatcatc cgcctcacct cgagatcgtc
     7021 gggacgaatc gcggaccgcc gaaatattgc cgatgggtct gctggttcaa gcttctcgac
     7081 aacccctata tcggcttcgt caaatccaac ggctacgaga aactgtcggg aacgaaagtc
     7141 tggacccgga cgggcgacga ctcgatcgat tggacgacga tcaattggcg ctgccgcgct
     7201 cggccgccgg cgacgagtta gttggctgtc attgcgagca aagcgaggca atccattcgc
     7261 agggctgcgc tatagattgc ttcgccgctg cgcgcctctt aaatgacggc gcaggctgcg
     7321 actcctctgg accgaatcct taaggcttcg cctcctccca tcccgtcggc gtccagcgca
     7381 gcaccgccgt cgagagcggc ttctgcgccc gcgccgcgac aaaggccgcg gcctcggcga
     7441 gcgcggcgac ggcgtcgttg tcgcggccgt tagcgtagat gagcgcgtcg ttcgccggcg
     7501 cggcgaccag gagatcgcca tcgacgctct cggcaatcgc cgtccagcgc tcgggaaaga
     7561 tcagccagct cgtaacgtag ggatcgccgg cgacgaggtt gacgcctggc cagggatagt
     7621 cgcgccgaaa aggctcgagc ggcggcaagg cggcggcgac gttctgcttg cacgccgcca
     7681 acgcctgctc acgcgagagt ttcagcgtcg cgagctcgtt cgcgttgaga attgcgaccg
     7741 cgcccggctg atcttgcgcg cacatgaccc agagaccgcc gatcagcctc tcggaaacgc
     7801 tcgctccgcc gcggcgggca taggccttgt cggctgcctc caccatggcc gccggccgta
     7861 cgatggcgcg aagctgcgac cgctcgatct tatccggcgc tttgaaactc gccgccatgc
     7921 ttgcgacatg cgagacgagg ccttcttcgc aagcctctgg attgcgtaag cagaaggaat
     7981 atacattgtt gagccatagg ctctcggcgc cgcggccggg tagggcgatc gacaaagcga
     8041 gcgctccctt gacctcgact ttggcctccg gcagcgcctt ggcgaagagc gcggccgcgt
     8101 gagcggtgaa gaagtcgacg tcgctgagat cggctgacat gggcgcatcc ttggccaagc
     8161 agcttgaggc aaggagcgcc atcgccaagg cggaggcggc ggcgatgagc gaggccacca
     8221 cgcctcgcct gccgagcgcg ccgaatccag atcgttcccg catcgctttc gccctcccgt
     8281 cggaccgctt cagcaggcgt tgaaattccg ccgcgccggc gcgccaaact cgctctacgg
     8341 cttcgcctcc gcccatccgg tcggcgtcca gcgaaacacc gcggtggaca gcggcttctc
     8401 tgcgcgcgcc gaaatcaagg cggcggcctt ggccagcgtg gcgacggaat catcatcttg
     8461 gccgctggca tagacgagcg tatcgaccgc c
//
LOCUS       DATGJW010000248         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1084243, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000248 DATGJW010000000
VERSION     DATGJW010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084243"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..1073)
                     /locus_tag="VKU03_07710"
     CDS             complement(108..1073)
                     /locus_tag="VKU03_07710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71186.1"
                     /translation="MQWFSSLTLPDNWKLQAIVYIAIAIIAIIAIAIAYRALFAHRLR
                     LPGGGRARQSRLGLVDAFSLDGQRQLVLVRRDNVEHLVMIGGPNDVVVESQIIRVHAQ
                     APAQLRDKESAPLQNASAPKIAAPAQPPAPAAAPFPHPQPQPQPHPHPPLSPARPQPR
                     PEPPNIAAQTRAAPKAPDVAVAARAPSPLPPPRPIAASPTPAPAPSVSPAPAPAAPLP
                     PTPAAAPAAAPSPAAPARIKNSSLPPPIVVASPPPSQPTKATASFAATAPAPAAAAPP
                     PKEAPPQKEKESPPKPAAAPPAKPEPFSDLDSLEAEMAKLLGRTN"
     gene            1454..>2124
                     /locus_tag="VKU03_07715"
     CDS             1454..>2124
                     /locus_tag="VKU03_07715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLJ71187.1"
                     /translation="MSQAPPPAAIDRVDRGANPGSLLLLTVCLVAAICAFYFLPAGQG
                     AKLAFWMITFLAVIGIFALLFYTFGFLLFTGQAARNDTTKVIADSSAEGLLVTDADSR
                     VLYANEAYMTLSGAHDVRDLKVVERLFTGAPEISEAIYRLAQAAREGKRGAEELRMSP
                     PLTGDAAVGWYRIRVRPLEQLGRGRSTLWTVADITRERERHENVFQELQHAIDYLDHA
                     PAGFFS"
BASE COUNT          317 a          685 c          766 g          356 t
ORIGIN      
        1 gcaggagcgc catgaacgca gagagcgcgt ttatcgggac gattgaggga aggcgaaccg
       61 gcgcgagccg gcttttttat gcgtccttga acggccgccc gccggcgtca attggtacgg
      121 ccgagcagct tggccatttc cgcttcgagc gaatcgagat cggagaaggg ctccggcttg
      181 gcgggcggcg ccgcggcggg tttcggcggc gattcctttt ccttttgcgg cggcgcctct
      241 ttgggcgggg gcgccgcggc ggccggcgcc ggcgcggtcg ccgcgaagct ggccgtcgcc
      301 ttggtcggct gagacggggg cggcgaggcc acgacgatcg gcggcggaag cgacgaattt
      361 tttatccgcg cgggcgcagc cgggctcggc gcggcggcgg gagccgccgc cggcgtgggc
      421 gggaggggcg cggccggagc aggagccggg ctcacgctcg gcgcgggcgc cggggttgga
      481 gacgccgcga tcggacgagg cggcggcagc ggcgagggcg cccgcgccgc gaccgcaacg
      541 tccggcgctt tcggcgcggc gcgggtctgg gcggcgatgt tgggcggctc cggccgcggc
      601 tgcggacggg cgggcgagag cggcgggtgc ggatgcggtt ggggctgcgg ctgcggatgc
      661 ggaaacggcg cggcggcggg cgcgggcggt tgagccggcg ccgcgatttt cggcgcgctc
      721 gcattttgca gcggcgcgct ctctttatcg cgaagctgcg ccggcgcctg agcgtgcacc
      781 cgaatgatct gcgattcgac gacgacgtcg ttcgggccgc cgatcatcac gagatgctcg
      841 acattgtcgc gccgcaccag aacgagttgg cgctgaccgt ccaaactgaa cgcgtcgacc
      901 aagccgagcc gcgattgccg cgctcttccg ccgccgggga ggcgaagccg atgcgcaaag
      961 agcgcccgat aggcgatggc gatggcgatg atcgcgatga ttgcgatggc gatatagacg
     1021 atcgcctgaa gcttccaatt gtccggcaaa gtcaggctac tgaaccattg catgtgaagt
     1081 cccgaaccgt catgcgcctc gtcggcctct caaagccggc tcaaccgacc gacacgcaaa
     1141 tggcgcgggc gccgaaaaag gcggacagcg cgtctgcgcg tcgacttgcc gaccgactac
     1201 gccaggcgca ttgccgccga tactcaaagc ggatgatttg cgctttcgtc aatagcaaaa
     1261 gcggcggcgc ccggcgcggc caatcgccgg cgccccggcg caaagcgacg tttttgcgcc
     1321 gccgccattg ccatggcgga aggccggccg cattgttgtc gaatcgatta ttttgctgga
     1381 agtctctgcg ctcgcgacgt tttggttggg gagacgccag atttctcaaa attccgctgt
     1441 ggaaagtcgc gcgatgagcc aagctccgcc gcctgccgcg atcgaccggg tcgaccgcgg
     1501 cgccaatccc gggtcgctgc tcctgctgac ggtttgcctc gtcgcagcga tttgcgcctt
     1561 ctatttcctg ccggccgggc agggcgccaa gctcgccttc tggatgatca cctttcttgc
     1621 ggtgatcggc attttcgcgc tgctcttcta cacgttcggc ttcctgctgt tcaccgggca
     1681 ggcggcgcgc aacgatacga ccaaggtgat cgccgacagc agcgccgagg gattgctcgt
     1741 caccgacgcc gattcgcgcg tcctctacgc caacgaggct tatatgacct tgtcgggcgc
     1801 ccacgacgtc agggatctca aggtcgtcga gcgcctgttc accggcgcgc cggaaatttc
     1861 cgaggcgatc tatcgcctgg cgcaggccgc gcgggagggc aagcgcggcg ccgaggagtt
     1921 gcgcatgtcg ccgcccttga ccggcgacgc cgcggtcggc tggtaccgca tccgcgtgcg
     1981 cccgctcgaa cagctcggtc gcggccgctc gaccttgtgg acggtggctg acatcacccg
     2041 cgagcgcgag cgccacgaga acgtcttcca ggagctgcag cacgcgatcg actatctcga
     2101 tcacgcgccg gccggcttct tctc
//
LOCUS       DATGJW010000249         6093 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_254447, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000249 DATGJW010000000
VERSION     DATGJW010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6093)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6093)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6093
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254447"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            240..1703
                     /locus_tag="VKU03_07720"
     CDS             240..1703
                     /locus_tag="VKU03_07720"
                     /inference="COORDINATES: protein motif:HMM:NF028467.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLJ71188.1"
                     /translation="MLSEAIDDLSIFSSTIPAGREQKRTAQTVLLACILVFLAAAPFA
                     RLALAPVWAFVPAYETVIATNDLITAVLLFGHFRHAGSRDHYILACGFLLTGFLAVAH
                     ELSFPGVFVATGLFGDHGQTTPWLYMFWHASLPVFVIVYAGMKDGAGMTPPQPWRPRY
                     AVLVAVAAVVALTALAIALPALLPQIAPANGDATLMTIVVSDVLGLGLFALAIMWLRR
                     PYFVLDLWIMVVMCASICEVALSAALNAGRFDLGFYIGCAYGLFGSSVMIMALPLENG
                     RLYERLKEAAGELGRLATTDPLTGVANRRAFEKVLDVEWRRAARRRLPLSLALIDIDF
                     FKNYNDCYGHPAGDQCLKAVATALANNTRRAGEMTARYGGEEFALLLPHADLAEARRL
                     AERVCYAVAALNIPHAQSPAAPCVTVSIGVAEMSAEIADAISARGVLGLASTKLIEAA
                     DKALYAAKAMGRNRVCVFSASPEGGADESAALPTAAE"
     gene            2015..2155
                     /locus_tag="VKU03_07725"
     CDS             2015..2155
                     /locus_tag="VKU03_07725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71189.1"
                     /translation="MTVQFIETEAGRLAVLPEAEYRRLADAAEDVVDAAAIDRFREKL
                     AA"
     gene            2192..2395
                     /locus_tag="VKU03_07730"
     CDS             2192..2395
                     /locus_tag="VKU03_07730"
                     /inference="COORDINATES: protein motif:HMM:NF024949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ71190.1"
                     /translation="MLAGESPVRVWREWRGLSARALAEAAGVTSAFLSQIEGGQRDGS
                     FDTMRKLAATLGVTLDDLAAPAE"
     gene            2492..3340
                     /locus_tag="VKU03_07735"
     CDS             2492..3340
                     /locus_tag="VKU03_07735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HLJ71191.1"
                     /translation="MYARPADLEAALALLAEPGARILAGGTDVFPAAGERPLSGRHVD
                     VTGIKGLRALRVAPDAIRIGAALTWSDIARADLPPAFDALKEAAREVGGAQIQNRGTI
                     AGNLCNASPAADGVPPLLVLDAEIEIASLRGARRLPLAQFIAGNRRTALAPGEIVTAI
                     VVPAPTPGMGSIFLKLGARRYLVISIVMVAILLDIDSSVVREARVAVGACSAAAMRLP
                     AVERRLAGAPARAGLGDIVAAEDLAAVAPIDDLRATAEYRRDAALTLVRRAVEARLAG
                     QAGGIV"
     gene            3340..>6093
                     /locus_tag="VKU03_07740"
     CDS             3340..>6093
                     /locus_tag="VKU03_07740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325876.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HLJ71192.1"
                     /translation="MLDRAPGVSLTLNGAPARLKSSPLERLSLALREECGLTGVKVGC
                     DAGDCGACTVLIDGEPVCACLVAVGQAEGRTIETVESLAKGGAIVGRLTAAFHAHGAA
                     QCGVCTPGMLLAATALLRKNPAPDESETQDALGGVLCRCTGYRKIIDAVRAAHRFEAE
                     ARLDLPPERAVGRRVARLDGARKISGAEIFGADEWPAGALIARAVRSPHHHARFVFGD
                     LDAFKAAHPGIAAVFTAKDVPGENCYGVIAPFADQPALAESVARFRGEAVALIVGEEA
                     AMRRLDLSAFPVDWEALPALMTIDEAIQASAALIHQNRPGNILTSGRVARGDVEAALA
                     SADAVVEGEFETGFVEHAYIEPEAGFARRAGDRIEIQACTQAPYMDRGDIAKILGIAP
                     EQVRIVPTAVGGGFGAKLDLSIQPFIALAAWRLDRPVRMVYSRQESIMTTTKRHPARI
                     RAKVGAARDGRLVAMDFSADFNTGAYASWGPTVANRVPVHASGPYLMPHYRARTRAIH
                     THCAPAGAFRGFGVPQSAIAQEQLYDELALKLGMDRLEFRILNALQAGDATVTGQVFK
                     EGVGFKACLEALRPRWREALDAARAFNDGAAGPLRRGVGIAGMWYGCGNTSLPNPSTM
                     RLGLKPDGRLTLFQGAVDIGQGSNTVIPQICADALGAPLALFDLASADTDVTPDGGKT
                     SASRQTFVSGKAAFLAGQAMRGEILRLANVGEAATIRFDGAAIVMAGAGGERQIDLRT
                     LSTNASGFVAEVQERFDPPTTPLDENGQGEPYAVFGYGAHLAEIEVDIELGRVKVLRL
                     TCAHDVGKAINPTLVEGQIEGGAAQGLGMALMEEFFPGKGENLHDYLIPSIGDMPPVE
                     SILIEDASPIGPFGAKGIGEQALIPTAPAILNAIHHATGARMRRAPATPDRLRAAILA
                     LQRA"
BASE COUNT          912 a         2125 c         2007 g         1049 t
ORIGIN      
        1 cgctccatca aatactgcgc taattttctt agaatacgaa tataccaaga aaactatatc
       61 cgtcgtccga cggtcgatcg ttggttgcac aatcggttta atccaaaata cactctgctc
      121 gacttgaagc tgtggggttg cgttaagcgt tatctttgga cggatcggcc gatcggaggg
      181 gcggtcggcg tctcgcccgg gatggtacgc gttatgcgcc caagcgaagc ggacggcgca
      241 tgctcagcga agccatcgac gatctttcga tcttttcctc caccatcccg gcggggcggg
      301 agcagaagcg gacggcgcag accgtcctcc tcgcctgcat tctcgtcttc ctcgcggcgg
      361 cgcccttcgc ccgcctcgcc ttggcgccgg tctgggcctt cgttccggcc tacgagacgg
      421 tgattgcgac caacgatctc atcaccgcgg tcctgctgtt cggccatttc cgccacgccg
      481 gctcgcgcga ccattacatt ctcgcctgcg gctttctgct gacgggcttt ctcgccgtcg
      541 ctcacgaatt gtcgttcccc ggcgtgttcg ttgcgaccgg ccttttcggc gatcacggcc
      601 agacgacgcc gtggctctat atgttctggc acgccagcct gccggttttc gtcatcgtct
      661 atgcgggaat gaaggacggc gccggcatga cgccgcccca gccgtggcgg ccgcgctacg
      721 ccgttctcgt cgccgtcgcc gccgtcgtcg cgctgaccgc cctagcgatc gccctgcccg
      781 cgctgctgcc gcagatcgcg ccggccaatg gcgatgcgac cctgatgacg atcgtcgtct
      841 ccgacgtact cggcctcggc ctcttcgcgc tcgccatcat gtggctgcgg cggccctatt
      901 tcgtgctcga tctctggatc atggtggtga tgtgcgcgtc gatctgcgag gtcgcgcttt
      961 cggcggcgct gaacgccggc cgcttcgatc tcggcttcta tatcggctgc gcctacgggc
     1021 tgtttgggtc gagcgtgatg atcatggcgc tgccgcttga aaacggcagg ctctatgagc
     1081 ggctgaaaga ggcggccggc gagctcgggc gcctcgccac caccgacccc ctgaccggcg
     1141 tcgccaatcg ccgggccttc gagaaggttc tcgacgtcga gtggcggcgc gcggcgcggc
     1201 gccggctgcc gctctcgctg gccttgatcg atatcgactt cttcaagaac tacaacgatt
     1261 gctacggcca tccggccggc gaccagtgcc tcaaggcggt ggcgaccgcc ctcgccaaca
     1321 atacgcggcg cgccggcgag atgacggcgc gctatggcgg ggaagaattc gcgctgctgc
     1381 tgccgcatgc cgacctcgcc gaggcgcgtc gcctggccga acgcgtctgc tatgcggtcg
     1441 cggcgctgaa cattccccat gcccagtcgc ccgccgcgcc ctgcgtcacg gtgagcatcg
     1501 gggtggccga gatgtcggcc gagatcgccg acgcgatcag cgcccgcggc gtgcttggcc
     1561 ttgcttcgac gaagctgatc gaagcagccg acaaggcgct ctatgcggcc aaggcgatgg
     1621 gccgcaaccg cgtctgcgtc ttctccgcct cgcccgaggg cggcgccgac gagagcgccg
     1681 cgctgccgac cgcggcggag tagttgacga cgtttttcac gccttctcgt ttccctgcgc
     1741 gcatggatta gcctatagct agattgttct tattcattgg ctatatgcta atacccgtcc
     1801 gaatgagcgc attgcgtacc gcaaagacgc gtttcgtacg ctgaagcgcc tgcccgccga
     1861 tccggccgtt ttggcgaaca atctcaaacc actcaaaggg cggctggcga actccgcctt
     1921 cgcgtcggcg actggcggat catcatgacg gaagatgcgg agactgtcgc ggtgatcaag
     1981 atatcgcgcg gttcggtcta tgaggagtga gaacatgacc gtgcaattca tcgagaccga
     2041 ggccggccgc ctcgccgtgt tgcccgaggc cgaatatcga aggctcgccg acgcggccga
     2101 ggacgtcgtc gacgcggctg cgatcgaccg cttccgcgaa aagctcgccg cctgagagga
     2161 ggagttcatc ccggccgaga tcgtctcccg tctgctcgcc ggcgaaagcc cggtacgggt
     2221 ctggcgcgaa tggcgcggcc tgagcgcgcg agccttggcc gaggcggccg gcgtcacgtc
     2281 ggcctttctg tcgcagatcg agggaggcca gcgcgatggc tctttcgaca cgatgcgcaa
     2341 gcttgccgcg acgctcggcg tcacgctgga cgatctcgct gcgccggcgg aatgagaacc
     2401 ggaaatcgtt ttatgtgcct aagctcggct tgacaaagcg ccctatcggc ataatcattt
     2461 gtatacaaac gagaccgagg gagtcgatct cgtgtatgcg cggcccgccg atctcgaagc
     2521 agccctggcc ttgctcgccg agcccggcgc gcgaattctc gccggcggga ccgacgtctt
     2581 cccggcagcc ggcgaaaggc cgctgagcgg ccggcacgtc gacgtgacgg ggatcaaggg
     2641 tttgcgcgcg ctccgcgtcg cgcccgacgc gatccgcatc ggcgccgcgc tcacctggtc
     2701 cgacatcgcc agagccgatc tgccgcccgc cttcgacgcg ctgaaggaag cggcgcgcga
     2761 agtcggcggc gcgcagatcc aaaaccgcgg cacgatcgcc ggcaatcttt gcaacgcctc
     2821 gccggcggcc gatggcgtcc cgccgctgct ggttttggac gccgaaatcg agatcgcctc
     2881 gcttcgcggc gcgcggcgcc tgcctctcgc tcaattcatc gccggcaatc gccgcaccgc
     2941 cctcgcgccg ggcgagatcg tcaccgcgat cgtcgtcccc gccccgacgc ccggcatggg
     3001 ctcgattttt ctgaagctcg gcgcgcggcg ctatctcgtc atttcgatcg tcatggtcgc
     3061 catcttgctc gacatcgaca gcagcgtcgt gcgcgaggcg cgcgtcgccg tcggcgcgtg
     3121 ctcggcggcg gccatgcggc tgcccgcggt cgagcgccgc ctcgccggcg cgcccgcgcg
     3181 cgccgggctc ggcgacatcg tcgcggccga agacctcgcc gccgtcgccc cgatcgacga
     3241 tttgcgcgcc acggccgagt atcggcgcga cgcggcgctg accttggtcc gccgcgcagt
     3301 cgaagcgcgc cttgcgggcc aagcaggagg aatcgtttga tgctcgaccg cgcgcctggc
     3361 gtctcgctca cgctgaacgg cgcgccggcg cggcttaagt cctcgccgct cgaaaggctg
     3421 agcctggcgc ttcgcgagga atgcgggctc accggggtca aggtcggctg cgacgccggc
     3481 gattgcggcg cctgcacggt gctgatcgac ggcgagccgg tctgcgcctg cctcgtcgcg
     3541 gtcggccagg ccgaaggacg gacgatcgag acggttgaaa gcctggcgaa aggcggggcc
     3601 atcgtcggcc gcctcacggc ggcttttcat gcgcatggcg cagcccagtg cggcgtctgc
     3661 acgcccggca tgctgctcgc cgcgaccgcc ttgctgcgca agaatccggc gcccgacgag
     3721 agcgaaacgc aggacgcgct tggcggcgtg ctctgccgct gcaccggcta tcgcaagatc
     3781 atcgacgcgg tgcgcgcggc gcatcgcttc gaggcggagg cgcgcctcga tctcccgccg
     3841 gagcgcgccg tcgggcgccg cgtcgcgcgg ctcgacggcg cgcgaaaaat ctccggcgcg
     3901 gagattttcg gcgccgacga atggccggcc ggcgcgctga tcgcccgcgc ggtccgctcg
     3961 ccgcatcacc acgcgcgctt cgtcttcggc gatctcgacg ccttcaaagc cgcgcatccc
     4021 ggaatcgccg cggtcttcac ggcgaaggac gtgcccggcg agaattgcta cggcgtgatc
     4081 gcgcctttcg ccgatcagcc ggctctcgcc gagagcgtcg cgcgctttcg cggcgaagcc
     4141 gtcgcgctga tcgtcggcga agaggcggcg atgcggcgcc tcgacctttc cgcctttccg
     4201 gtcgattggg aggcgctgcc ggccctgatg acgatcgacg aggcgattca ggcgagcgcc
     4261 gcgctgatcc atcagaatcg gcccggcaat attctgacaa gcggccgcgt cgcgcgcggc
     4321 gacgtcgagg cggcgctcgc gtccgccgat gcggtcgtcg agggcgagtt cgagaccggc
     4381 ttcgtcgagc acgcctatat cgagcccgag gccggcttcg cccgacgggc cggcgaccga
     4441 atcgaaatcc aggcctgcac ccaggcacct tatatggacc gcggcgatat cgccaagatt
     4501 ctcgggatcg cgcccgagca ggtgcggatc gtcccgacgg cggtcggcgg cggcttcggc
     4561 gccaagctcg atctttcgat ccagcctttc atcgcgcttg ccgcctggag gctcgatcgt
     4621 ccggttcgca tggtctattc gcgccaggaa tcgatcatga cgacgaccaa gcgccacccc
     4681 gcgcgcatcc gcgcgaaagt cggcgccgcg cgggacggcc ggctcgtggc gatggacttc
     4741 tccgccgatt tcaacaccgg cgcctacgcc tcctggggcc cgacggtcgc caatcgcgtg
     4801 ccggttcacg cctcgggtcc gtaccttatg ccgcattacc gcgcgcggac gcgtgcgatc
     4861 catactcatt gcgcgccggc cggcgcgttc cgcggcttcg gcgtgccgca aagcgcgatc
     4921 gcgcaggagc agctctatga cgagctggcg ctcaagctcg gcatggaccg gctcgaattc
     4981 cgcatcctca acgccttgca agcgggcgac gccacggtga cgggccaggt cttcaaggaa
     5041 ggcgtcggct tcaaagcctg tctcgaagcg ttgcggccgc gctggcgcga ggctttggac
     5101 gcggcccgcg ccttcaacga cggcgccgcc ggcccgctgc gccgcggcgt cggaatcgcc
     5161 ggcatgtggt acggctgcgg caacacgtcc ctgcccaatc cctcgaccat gcgcctcggc
     5221 ctgaagcccg acgggcggct gaccttgttc caaggcgcgg tcgatatcgg ccaaggctcc
     5281 aacaccgtca tcccgcaaat ctgcgccgat gcgctcggcg cgccgctggc gctgttcgac
     5341 ctcgcctcgg ccgacaccga cgtcacgccg gacggcggca agacctcggc ctcgcgccag
     5401 actttcgtct cgggcaaggc cgcctttctc gccggccagg cgatgcgcgg cgagattctg
     5461 cgcctcgcca atgtcggcga agcggcgacg atccgcttcg acggcgcggc gatcgtcatg
     5521 gccggcgcgg gcggcgagcg ccagatcgat cttcgcactc tttcgaccaa tgcgtccggc
     5581 ttcgtcgccg aggtacagga gcggttcgat ccgccgacca cgccgctcga cgagaacggc
     5641 cagggcgagc cttacgccgt gttcggctat ggcgcgcatc tcgccgaaat cgaagtcgat
     5701 atcgagctcg gccgcgtaaa ggttctccgc ctgacctgcg ctcacgacgt cggcaaggcg
     5761 atcaacccga cgctggtcga aggccagatc gagggcggcg cggcgcaagg cctcggcatg
     5821 gcgctgatgg aggagttctt ccccggcaag ggcgagaacc tgcacgatta tctcattccc
     5881 agcatcggcg acatgccgcc ggtcgaaagc atcctgatcg aggacgcgtc cccgatcgga
     5941 ccgttcggcg ccaaggggat cggcgagcag gctttgattc cgaccgcgcc ggcgatcctc
     6001 aatgcgatcc atcatgcgac cggcgcccgg atgaggcgcg cgccggcgac gccggaccgc
     6061 ttgcgcgccg cgatcctcgc tttgcaaagg gcc
//
LOCUS       DATGJW010000250         8190 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_254663, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000250 DATGJW010000000
VERSION     DATGJW010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8190)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8190)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8190
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_254663"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..282
                     /gene="mraW"
                     /locus_tag="VKU03_07745"
     CDS             <1..282
                     /gene="mraW"
                     /locus_tag="VKU03_07745"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytosine(1402)-N(4))-methyltransferase"
                     /protein_id="HLJ71193.1"
                     /translation="SLEDRIVKQFFARRSGRGQAAGRRLPGEKPAPAPTFVVAAGQPI
                     APGEAERSANPRARSAKLRFGTRTDAPARGADDALTALTEASRRTTRRA"
     gene            282..653
                     /locus_tag="VKU03_07750"
     CDS             282..653
                     /locus_tag="VKU03_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71194.1"
                     /translation="MWRIMHIVAIGALVASAVYVYTIKYKTILWSEKIVETRHAIERE
                     QDAINILRAEYALLTRPERLQALADKDLGMQPLALNQIVKASDLPEAQPKIDSIGRKI
                     ESLGLSATPSSGLTGATPSTR"
     gene            708..2603
                     /locus_tag="VKU03_07755"
     CDS             708..2603
                     /locus_tag="VKU03_07755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein 2"
                     /protein_id="HLJ71195.1"
                     /translation="MDAKNPFSRSREKGAAKRPDEAAAEASDRDGSPSPQPSPASGRG
                     GAPRPLQAPRSLLARLGALAGEIFRARIDKSGGRIRLIAFGLFLIYAGIGGRLILLGL
                     SHEPPQTLKTAADQAVSGARPDLLDRNGEILATDIKTMSVFAEPRRIIDKDEAVELIT
                     AVLPDVNAKELRERLSSKKGFVWVKRQVTAKEQAEIFHLGLPGVGFLPENKRVYPNGP
                     IGAHVIGYVDKDNIGIAGMEKYLDNQSLTDPHVAGFSVDPASLKPVRLSLDLRVTHAL
                     RDELQWGLERFHAKAAAGAVMDVTTGEVIAAESLPDFDPNDPGDMRDPMLINRLTVGV
                     YEMGSTFKALTLAMALDSGKVTLGTRVDARENLRYGRFTIHDFHATHRVLTVPEVFTH
                     SSNIGSARLALMIGVQGHQAFLRKAGQLDRMRTELPESAEPLVPKHWSELNTMTIAFG
                     QGLNVAPLQAMMAVAALVNGGNLMTPTFLTRSPAEALAHSRRLVSAQTSESMRYLLRA
                     NATHGSAGFANIPGYYVGGKTGTAQKNFHGHYSNDKVFTTFMAVVPADKPKYLYMAIY
                     DDPQAAPGDGGYHTAAYNAGRVTGRLVERIEPLLGVPPSSEPPTQPFPLIARLGYGGD
                     ADRIGKE"
     gene            2603..4048
                     /locus_tag="VKU03_07760"
     CDS             2603..4048
                     /locus_tag="VKU03_07760"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021721.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HLJ71196.1"
                     /translation="MKLRDLFGPGLDAALAGREAAGLCVDSRKAKEGDVFFALSGAKS
                     DGLEFVADAASRGAKIIVAERKPDQLPDDVELVRVADARAALARAASRFFPRQPATIV
                     AVTGTSGKTSVAEFTRQIWRELGFEAASLGTIGIVSPKTNVYGALTTPDPIALHRALD
                     DLAGAGVTHLAMEASSHGLDQKRLDGVRLVAGAFTNLSRDHLDYHGDLKSYLAAKLRL
                     FMELLAPGAAAVVDADSEAAGEVIAAARKRGLDLATVGKAGETIRLVAAERAGFANRL
                     TLSHGGRERRVLLPLPGDFQISNALVAAGLCLATGCDAQKVFAALEVLQGAPGRLERV
                     GETRGASVFVDYAHKPDALDKALAALRPFVFGRLIVVFGCGGDRDAGKRPIMGEIATR
                     RADVTIVTDDNPRSEDPAAIRAAILAAAPGAIEIGDRGEAIKSAIAMLRRGDALLIAG
                     KGHETGQIVGDRVLPFSDAEAARAALKDVAA"
     gene            4045..5463
                     /locus_tag="VKU03_07765"
     CDS             4045..5463
                     /locus_tag="VKU03_07765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoylalanyl-D-glutamyl-2,
                     6-diaminopimelate--D-alanyl-D-alanine ligase"
                     /protein_id="HLJ71197.1"
                     /translation="MSEALWTESELAQTLGAPTSAPAAPVRGVSIDTRTLTPGDLFFA
                     IKGDANDGHAYVERAFAAGAAASVVARDKADALAGCGALFAVDDTLRAMERLGALARR
                     RTKARIAAVTGSVGKTSAKEMLRVVLGKAGATHAAAASYNNHWGVPLTLARMPASAAF
                     GVFEIGMNHAGEIAPLVALVRPHAALVTTIAPVHIEHLGSLEAIAEAKAEIFTGLQPG
                     GAAILPRDAPQFDLLRRRAAAAGAGIATFGAGREADAQLLDCRPHGRGSRVEARIFGR
                     AVAFDLGAPGLHMAQNALGVVLVAHSLGVEIESSAASLADFAPAKGRGERIVLRANSG
                     AFTLIDESYNANPASMRAALDLLGATEPGRGGRRIAALGDMLELGPQGLAMHEALAAD
                     LKRNRVDLVFCAGLLMRGLYEAAPEAMRGAWAAKAADIEKPLFDALRGGDVVMVKGSN
                     GSRMGPVVAALKANFSGDASAA"
     gene            5477..6559
                     /gene="mraY"
                     /locus_tag="VKU03_07770"
     CDS             5477..6559
                     /gene="mraY"
                     /locus_tag="VKU03_07770"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012173060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HLJ71198.1"
                     /translation="MLTWLADFSHLFSPFNLFRYLTFRTIGAAGTGLFFVFFFGPAII
                     SALRLKQGKGQPIRLDGPQSHLLTKKGTPTMGGLMILSGLIVSALLWANLESPYVWIV
                     LFVTVGFGAIGFYDDYLKVTKQSDRGFSGRSRLACEFAIALLACLAMIYVGGPGATSL
                     AVPFVNGFVVDLGWGFLLFGAFVIVAFGNAVNLTDGLDGLAIVPVMIGAATLGLIAYL
                     VGNLFFARYLGINHVPHAGDLVVVCGALIGAGVGFLWFNAPPAQIFMGDTGSLALGGL
                     LGATAVAIKHEIVLAIVGGLFVVEALSVVVQVASFKMTGKRVFKMAPIHHHFEQLGWS
                     EPQIVMRFWIVAFVLAMVGLSTLKLR"
     gene            6561..7961
                     /gene="murD"
                     /locus_tag="VKU03_07775"
     CDS             6561..7961
                     /gene="murD"
                     /locus_tag="VKU03_07775"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890164.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HLJ71199.1"
                     /translation="MIPVAGFNGRKVALFGLGGSGLATARALLAGGAEVAAWDDGEAA
                     RTKAREAGLPVVDLAEADWRGFSALVLAPGVPLTHPEPHWTAKKARAAAIEIIGDIEL
                     FCRERRRIAPRAPFVAITGTNGKSTTTALVAHLLREAGRDAQLGGNIGTPILELAAPA
                     DNRIHVVECSSFQIDLAPSIDPTIGALINLTPDHLDRHGTIENYAAVKERLVAQAEIA
                     LVGLDDEFCRAIAERLGALQPAKGPRVVTISAEPSRSADIRFEGGAIRCGENRLDLAG
                     AEALRGAHNAQNAAFAAVAARELGISFGEIARGLKSFPGLAHRMEQVGKIGRTLFVND
                     SKATNSDAAEKALHSFSDIFWIVGGKAKEGGIEPLRPLFPKIAKAYLIGASSEVFAKT
                     LQGSVAFERCGTLDVATAAAARDAAASAAAEPVVLLSPACASYDQFANFEARGDKFRA
                     LVKDLIAAQKGADAHV"
     gene            7954..>8190
                     /locus_tag="VKU03_07780"
     CDS             7954..>8190
                     /locus_tag="VKU03_07780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71200.1"
                     /translation="MSEASIAFADADADRVAPPLPLAGEGWGEGERRRLLIIFFAAAY
                     PHPAALRASTFSRKWEKGLGCCAAYVDTAFASANE"
BASE COUNT         1266 a         2803 c         2698 g         1423 t
ORIGIN      
        1 tcgctcgaag accggatcgt caagcaattc ttcgcgcgcc gctccggccg cgggcaggcg
       61 gcggggcgcc gcctgcccgg cgagaagccg gctccggcgc cgaccttcgt ggtcgccgcc
      121 ggccagccga tcgcgccggg cgaggcggaa cgctcggcca atccgcgcgc gcgctccgcc
      181 aagctgcgct tcggaacccg caccgacgcg ccggcgcggg gagcggacga cgctctgacg
      241 gcgctgaccg aagcttcgcg tcgcacgacc aggagagcct gatgtggcgc atcatgcata
      301 tcgtcgcgat cggcgctctc gtcgcctcgg cggtctatgt gtatacgatc aagtacaaga
      361 cgatcctctg gtccgagaag atcgtcgaaa cgcgtcacgc gatcgagcgc gagcaggacg
      421 cgatcaacat cctgcgcgcc gaatacgcct tgttgacccg cccggagcgg ctgcaggcgc
      481 tcgccgacaa ggatctcggc atgcagccgc tggcgctcaa tcagatcgtg aaagcgtccg
      541 acctgccgga ggcgcagcca aagatcgatt ccatcggccg caagatcgag tcgctcggcc
      601 tttccgccac gccgagctcc ggcctgaccg gcgccacgcc gtcgacgagg tgagcgatgg
      661 atgagcgggg ttggaaatcg acggcatttg ggattgccaa gtccgtcatg gacgcgaaga
      721 accccttctc ccgctcgcgg gagaagggcg ccgcgaagcg gccggatgag gcggcggccg
      781 aagcctccga tcgcgatggg tcgccctcgc cccaaccctc tcccgcaagc gggagagggg
      841 gcgcgccgcg gcctttacag gcgccgcgct ccctgctggc gcggctcggc gcgctcgcgg
      901 gcgaaatctt ccgcgcccgc atcgacaaaa gcggcggccg cattcgcctc atcgccttcg
      961 gattattcct gatctatgcc ggcatcggcg gccggctgat cctgctcggc ctgtcgcacg
     1021 agccgccgca aacgctcaaa accgccgccg accaagccgt ctccggcgcg cggcccgatc
     1081 ttctcgaccg caacggggaa attctggcga ccgacatcaa gaccatgtcg gtcttcgccg
     1141 agccgcggcg catcatcgac aaggacgagg cggtcgaact gatcaccgcc gtgctgccgg
     1201 acgtcaacgc caaggagctg cgcgagcgcc tctcctcgaa gaaaggcttc gtctgggtca
     1261 agcgccaagt caccgccaag gagcaggcgg agattttcca tctcggcctg ccgggcgtcg
     1321 gctttcttcc ggagaataag cgggtctatc ccaacggccc gatcggcgcg catgtgatcg
     1381 gctatgtcga caaggataat atcggcatcg ccgggatgga gaagtacctc gacaatcaaa
     1441 gcctcaccga tccgcatgtc gccggcttca gcgtcgaccc ggcgtcgctg aagccggtgc
     1501 gcttgtcgct cgatctacgc gtgacccatg ctttgcgcga cgaattgcaa tggggcctcg
     1561 agcgcttcca cgccaaggcg gcggccggcg cggtgatgga cgtaacgacc ggcgaggtga
     1621 ttgcggcgga atcgctgccg gatttcgatc cgaacgatcc cggcgacatg cgcgacccga
     1681 tgctgatcaa ccgcctcacc gtcggcgtct atgaaatggg ctcaaccttc aaggcgctga
     1741 ccctcgccat ggcgctcgat tccggcaagg tgacgctcgg cacgcgcgtc gatgcgcgcg
     1801 agaaccttcg ctacggccgc ttcaccattc atgacttcca cgcgacgcat cgcgtcctga
     1861 ccgtgccgga agtcttcacc cattcctcga atatcggctc ggcccgcctc gccttgatga
     1921 tcggcgtgca aggccaccag gcgttcctga gaaaggccgg acagctcgac cggatgcgca
     1981 ctgaattgcc ggaaagcgcc gagccgctcg ttccgaaaca ttggagcgag ctcaacacga
     2041 tgacgatcgc cttcggccag gggctcaatg tcgcgccgct ccaggcgatg atggccgtcg
     2101 cggctctggt taatggcggc aacctgatga cgccgacctt tttgacgcgc agcccggccg
     2161 aggccttggc ccatagccgc cgcctggtca gcgcgcagac ctcggaatcg atgcgctatc
     2221 tgttgcgcgc caacgccacc catggctcgg cgggcttcgc caatattcca ggctattatg
     2281 tcggcggaaa aaccggcacc gcgcagaaga atttccacgg ccactattcc aacgacaagg
     2341 ttttcacgac gtttatggcc gtggtgcctg ccgacaagcc caaatatctg tatatggcga
     2401 tctacgacga tccgcaggcc gcgccggggg atggcggtta tcacaccgcg gcgtataacg
     2461 ccggccgggt gaccggccgg ctggtcgagc ggatcgagcc gcttttgggc gtgccgccct
     2521 cttcggagcc gccgacgcag ccgttccccc tcatcgcgag gctcggctat ggcggcgacg
     2581 cggataggat aggcaaggag tgatcaagct acgcgacctt ttcggccctg gccttgacgc
     2641 cgcgctcgcc gggcgcgaag ccgccggcct ttgcgtcgac agccgcaagg cgaaggaggg
     2701 cgatgttttt ttcgccttgt ccggcgccaa gagcgacggg cttgaattcg tcgccgacgc
     2761 tgcttcgcgc ggagcgaaga tcatcgtcgc cgagcgtaag ccggatcaac ttcctgacga
     2821 tgtcgaattg gtgcgcgtcg ccgacgcccg cgcggcgctc gcgcgcgccg cgtcgcgctt
     2881 ctttccgcgc caaccggcga cgatcgtcgc ggtgaccggc accagcggca agacttcggt
     2941 tgcggaattc acccgccaga tctggcgcga actgggcttc gaagccgctt cgctcggcac
     3001 gatcggcatc gtctcgccga agaccaatgt ctatggcgcg ctgaccacgc ccgatccgat
     3061 tgcgctgcac cgcgcgctcg acgatctcgc cggcgccggc gtcacgcatc tggcgatgga
     3121 ggcctcttcg cacggcctcg atcagaagcg gctcgacggc gtgcgcctcg tcgccggcgc
     3181 cttcaccaac ctgtcgcggg accatctcga ctatcacggc gatctcaaat cgtacctcgc
     3241 cgccaagctg cggctgttca tggagctgct cgcgccgggc gcggcggcgg tcgtcgacgc
     3301 cgatagcgag gcggcgggcg aggtcatcgc cgccgcgcgc aagcgcgggc tcgatctggc
     3361 cacggtcgga aaagccggcg agacgatccg gctcgtcgcc gccgagcggg ccggcttcgc
     3421 caatcggctg acgctcagcc atggcggccg cgagcgccgc gtccttttgc ccctgcctgg
     3481 cgattttcaa atctccaacg cgctcgtcgc cgcgggcctc tgtctcgcca cgggctgcga
     3541 cgcgcagaag gtcttcgccg cgctcgaagt cctgcagggc gcgccgggcc ggctcgagcg
     3601 ggtcggcgag acgcgcggcg cgtccgtctt cgtcgactac gcgcacaagc ccgacgcgct
     3661 cgacaaggcg ctcgccgcct tgcgtccttt cgtcttcggc cggctcatcg tcgtctttgg
     3721 ctgcggcggc gaccgcgacg ccggcaagcg tccgatcatg ggcgagatcg cgacgcggcg
     3781 cgccgacgtg accatcgtca ccgacgacaa tccccgttcc gaggatcccg ccgcgatccg
     3841 cgccgcgatt ctcgccgcag cgcccggcgc gatcgaaatc ggcgatcgcg gcgaagcgat
     3901 caaatcggca atcgccatgc tccggcgcgg cgatgcgctg ctgatcgccg gcaaaggcca
     3961 tgagaccggc cagatcgtcg gcgaccgggt cctgcctttc tccgacgcgg aagcggcgcg
     4021 ggcggcgctg aaagacgtcg ccgcatgagc gaggcgctgt ggacagaaag cgagcttgcg
     4081 cagaccctgg gcgcgccgac cagcgccccg gcggcgccgg tccgcggcgt ttcgatcgac
     4141 acgcgcacat tgacgccggg cgatctcttc ttcgcgatca agggcgacgc caatgacggc
     4201 cacgcctatg tcgagcgcgc cttcgccgcc ggcgccgcgg cttccgtcgt ggcgcgcgac
     4261 aaggccgacg ccctcgctgg ttgcggcgcg cttttcgcgg tcgacgacac gctgcgcgcg
     4321 atggagcggc tcggcgccct ggcgcgccgc cgtacgaagg cgcgaatcgc ggcggtgacc
     4381 ggctcggtcg gcaagaccag cgccaaggag atgctgcgcg tcgtcctggg caaagccggc
     4441 gcgacgcatg ccgcggccgc ctcctataac aatcattggg gcgtgccgct gaccttggcg
     4501 cgcatgccgg cttccgccgc tttcggcgtc ttcgagatcg gcatgaatca cgccggcgag
     4561 atcgccccgc tcgtcgcttt ggtgcgcccg catgcggctc tggtgacgac gatcgcgccg
     4621 gtccatatcg agcatctcgg ctcgctcgag gcgatcgccg aagccaaggc cgagattttc
     4681 accgggctcc agccgggcgg cgccgcgatc ctgccgcgcg acgcgccgca attcgatctg
     4741 ctgcggcggc gcgccgcggc ggcgggcgcg gggatcgcga cattcggcgc cggacgggag
     4801 gcggacgcgc agctcctcga ttgccggccg catggccgag gctcgcgcgt cgaggcgcgc
     4861 attttcggcc gcgccgtcgc tttcgacctc ggcgcgccgg gccttcacat ggctcagaac
     4921 gcgctcggcg tcgtcctcgt cgcccattcc ctcggcgtgg agattgagtc gagcgccgcg
     4981 tcgctcgccg actttgcgcc cgcgaaaggc cgcggcgagc ggatcgtcct tcgcgccaac
     5041 agcggcgctt tcacattgat cgatgagagc tacaacgcca atccggcctc gatgcgcgcc
     5101 gcgctcgatc ttctcggcgc gaccgagccg ggacgcggcg gccggcgcat cgccgctctc
     5161 ggcgacatgc tggagcttgg gccgcagggc ctcgccatgc acgaagcgct cgccgcggac
     5221 ctcaagcgca atcgcgtcga tctcgtcttc tgcgccgggc tcttgatgcg cgggctctac
     5281 gaagcggcgc ccgaagcgat gcgcggcgct tgggcggcca aggccgccga catagagaag
     5341 ccgcttttcg acgctttgcg gggcggcgac gtggtgatgg tcaaggggtc gaacggcagc
     5401 cggatggggc cggtcgtcgc cgcgctgaaa gcgaatttct ctggcgacgc cagcgccgca
     5461 taaacgggaa ttttcaatgc tcacctggct cgccgatttc tcgcatctgt tcagcccgtt
     5521 caatcttttt cgctatctca ccttccgcac gatcggcgcc gcgggaaccg ggctgttttt
     5581 cgtgttcttc ttcgggccgg cgatcatctc ggctctgcgc ttgaaacagg gcaaaggaca
     5641 gccgatccgg ctcgacgggc cgcaatcgca tcttctgacc aagaagggca cgccgaccat
     5701 gggcgggctg atgatccttt cgggtctcat cgtctccgct ctgctttggg ccaatctcga
     5761 aagcccctat gtctggatcg tccttttcgt cacggtcggc ttcggcgcga tcggcttcta
     5821 tgacgattat ctcaaagtga cgaagcaatc ggatcgcggc ttttccggcc gctcgcggct
     5881 cgcctgcgaa ttcgccattg cgctgctcgc ctgtttggcg atgatctatg tcggcggccc
     5941 gggcgcgact tcgctggccg tgccgttcgt caacggcttc gtcgtcgatc tcggctgggg
     6001 ctttctgctc ttcggcgctt tcgtcatcgt cgccttcggc aatgcggtca atctcacgga
     6061 tggactcgat gggctggcga tcgtgccggt gatgatcggc gcggcgacgc tcggcctcat
     6121 cgcctatctc gtcggcaatc tgttcttcgc ccgctatctc ggcatcaacc atgtgccgca
     6181 tgccggcgac ctcgtcgtcg tctgcggcgc gctgatcggc gccggcgtcg gctttctctg
     6241 gttcaatgcg ccgccggcgc agattttcat gggcgatacc ggctcgctgg cccttggcgg
     6301 cctgctcggc gccacggcgg tcgcgatcaa gcacgagatc gtgctggcga tcgtcggcgg
     6361 cttgtttgtg gtcgaggcgc tttcggtcgt cgtgcaggtc gcctcgttca agatgaccgg
     6421 caagcgcgtc ttcaaaatgg cgccgatcca tcatcatttc gagcagctcg gctggtccga
     6481 gccgcaaatc gtcatgcgct tctggatcgt cgccttcgtt ctcgccatgg tcggcctctc
     6541 gaccttgaag ctgaggtgag atgatcccgg tcgccggctt caacgggcgc aaagtcgcgc
     6601 tgttcggcct gggcggctcg ggcctcgcga cggcgcgcgc gctgctcgcc ggcggcgccg
     6661 aggtcgcggc ctgggacgat ggcgaggcgg cgaggacgaa ggcgcgcgaa gccggcctgc
     6721 cggtcgtcga cctcgccgag gcggattggc gcggcttttc cgctttggtc ctcgcgcccg
     6781 gcgtgccgct gacccatccc gagccgcatt ggacggcgaa aaaggcgcgc gccgcggcga
     6841 tcgagatcat cggcgacatc gaacttttct gtcgcgaaag gcgtcgcatc gcgccgcgcg
     6901 cgcctttcgt ggcgatcacc ggaaccaatg gcaagtcgac gacgacggcg ctcgttgcgc
     6961 atttgttgcg ggaggcgggt cgggacgcgc agctcggcgg caatatcggc acgcccattc
     7021 tcgaactcgc cgcgccggcc gacaatcgca tccatgtcgt cgaatgctcc tcgttccaga
     7081 tcgacttggc gccgtcgatc gatccgacga ttggcgcgct gatcaacctg acgccggacc
     7141 atctcgatcg tcacggtacg atcgagaatt acgccgcggt caaggaaagg ctggtcgcgc
     7201 aagccgagat cgctctggtc gggctcgacg atgaattctg ccgcgccatc gccgagcggc
     7261 tcggcgcgct tcagccggcc aaggggccgc gcgtcgtgac gatttctgcc gaaccgtctc
     7321 gttctgccga tattcgtttc gaaggcggcg cgattcgctg cggcgaaaac aggctcgacc
     7381 ttgccggcgc cgaagcgttg cgcggcgcgc acaacgcgca gaacgccgcg ttcgccgccg
     7441 tcgctgcgcg cgaactcgga atctctttcg gcgagatcgc gcgcgggctg aagagtttcc
     7501 ccggcctcgc gcatcggatg gagcaggtcg gcaagatcgg ccgcacgctc ttcgtcaacg
     7561 attcgaaggc gaccaactcc gacgcggcgg aaaaggcgct gcattccttc tcggatattt
     7621 tctggatcgt cggcggcaag gccaaggaag gcggcatcga gccgttgcgg ccgttgttcc
     7681 ccaagatcgc taaggcctat ctcatcggcg cgtcgagcga agtttttgcg aagaccctgc
     7741 aaggaagcgt cgcgttcgag cgctgcggga ccctggacgt cgcgacagca gcggcggcgc
     7801 gcgacgccgc ggcgagcgcc gcggccgagc ccgtcgtact gctctcgccg gcttgcgcct
     7861 cctacgatca gttcgccaat ttcgaagcgc gcggcgacaa gtttcgcgcc ttggtcaaag
     7921 acctcatcgc cgcgcagaaa ggggccgatg cgcatgtctg aggcgagtat cgcattcgcc
     7981 gacgcagatg cagaccgcgt cgcgccccct ctcccgcttg cgggagaggg ctggggtgag
     8041 ggcgagcggc ggcgcttgct gatcattttt tttgccgccg cgtaccctca tccggccgcg
     8101 cttcgcgcgt ccaccttctc ccgcaagtgg gagaagggat tgggctgctg tgcggcctac
     8161 gtcgacacgg cgttcgcgag cgcaaacgaa
//
LOCUS       DATGJW010000251         2624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1084628, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000251 DATGJW010000000
VERSION     DATGJW010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2624
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084628"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..259)
                     /locus_tag="VKU03_07785"
     CDS             complement(<1..259)
                     /locus_tag="VKU03_07785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015916347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HLJ71201.1"
                     /translation="MDAVATNGEAASVTISASAARRLAKILSSEEPGAALRVSVKGGG
                     CSGFQYSFDIDKSRADDDLIVERDGATILIDPVSLEYMAGAE"
     gene            353..1570
                     /locus_tag="VKU03_07790"
     CDS             353..1570
                     /locus_tag="VKU03_07790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016793 - triphosphoric monoester
                     hydrolase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyguanosinetriphosphate triphosphohydrolase"
                     /protein_id="HLJ71202.1"
                     /translation="MDGSSAKRAPYAADPAASRGRVVPEPPSPTRTDFQRDRDRIIHS
                     TAFRRLAHKTQVFVYHEGDHFRTRLTHTIEVSQIARALARALRLDEDLAEAVALGHDL
                     GHTPFGHTGEDALDACLAPYGGFDHNSHGLKIVTELERRYADFNGLNLTFETIEGLVK
                     HNGPFLDAGGAPLGRWRRRGVPPAILAYDRLRDLELAHYASLEAQAAAIADDIAYDAH
                     DIDDGLRAGLFDFAAARQAPFIAALLDEIERLHPGLEKSRVTHELTRRLITRFIEDAI
                     AESRARIRDAHILSLEDVRAAAKPLVGFSPLLAEAAEAIKAFLFANMYRHPSVARVRD
                     KAERLVRQLFAAFFAEPAKMPAEWAQLTAEAEMREGEAGRAQAVADYIAGMTDRYAVA
                     EHGRLFDEAPDLR"
     gene            1603..>2624
                     /gene="argS"
                     /locus_tag="VKU03_07795"
     CDS             1603..>2624
                     /gene="argS"
                     /locus_tag="VKU03_07795"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823564.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HLJ71203.1"
                     /translation="MNIFADFQRRIATLLEDIAASGGLPKPLDLGRFTVEPPREAAHG
                     DLSTNAAMVFAKEAKAAGSSPRALATEIAAGLAEQPDVDQAEVAGPGFINIRLKPVVF
                     ENVLRSVLAEGRRFGAGAAAGEPINVEYVSANPTGPMHVGHARGAVFGDALANLLSFA
                     GRSVTREYYINDAGAQVDALARSAYLRYREALGETVGAIPDGLYPGDYLVPVGAALAK
                     AFGKRLQEKPEAEWLPIVRREAIDAMMAMIRDDLAALNVRHDVFSSERALTGADGGRD
                     RVRETIEDLKARGIVYLGRLPPPKGAPVEDWEDREQTLFRSTDFGDDVDRPLIKSDGG
                     YTYFAA"
BASE COUNT          433 a          920 c          846 g          425 t
ORIGIN      
        1 cttcggcgcc ggccatatat tcgagcgaca ccggatcgat cagaatggtc gcgccgtcgc
       61 gctcgacgat gaggtcgtcg tcagcccggc tcttgtcgat atcgaacgaa tattgaaagc
      121 ctgaacagcc gccgcctttc accgagacgc gcaaggccgc gccgggctct tcgctcgaca
      181 gaattttggc gagccggcgg gccgcgctcg cgctgatcgt cacggaggcc gcctctccat
      241 tcgtcgcaac agcgtccatc ggtcaaacct tcgaagccgg gttcacaaaa gctctcgcgg
      301 agtaagatag gcttgcaggc gtgaagcttc aatctggggc cgggagcgcg aaatggatgg
      361 gtcatcggcc aaacgcgcgc cctatgcggc cgatcccgcc gcctcgcggg ggcgcgtggt
      421 cccggagccg ccatcgccga cccgcaccga cttccagcgc gaccgcgacc gcatcatcca
      481 ttcgaccgcg ttccggcgcc tcgcccacaa gacgcaggtt ttcgtctacc acgagggcga
      541 tcatttccgc acccgcctga cccacacgat cgaagtctcg caaatcgccc gcgctctcgc
      601 ccgggcgctg cggctcgacg aggacctggc cgaagcggtg gcgctcggcc acgatctcgg
      661 ccatacgccc ttcggccata ccggcgagga cgcgctcgac gcctgcctcg ccccctatgg
      721 cggcttcgac cacaacagcc acggcttgaa aatcgtcacc gagctcgagc gccgctacgc
      781 cgatttcaac ggcctcaatc tgacattcga aacgatcgag ggcctggtga agcataacgg
      841 gccattcctg gacgccggcg gcgcgccgct cgggcgctgg cgccggcgcg gcgttccgcc
      901 agcgatcctc gcctatgacc gcctacgcga cctggagctc gcgcattacg ccagcctcga
      961 agcgcaagcc gcggcgatcg ccgacgatat cgcctatgac gcccatgaca tcgacgacgg
     1021 cctgcgcgcc ggcctttttg atttcgccgc cgcccgtcaa gcgccgttca tcgccgcctt
     1081 gctcgacgag atcgagcggc ttcatcccgg cctcgaaaaa agccgcgtga cgcacgagct
     1141 cacccggcgg ctgatcacgc gcttcatcga ggacgccatc gccgagagca gggcgcggat
     1201 tcgcgacgct catatcctgt cgcttgaaga tgtccgcgcg gcggccaagc cgctggtcgg
     1261 cttctcgccg cttcttgccg aggcggcgga ggcgatcaag gcctttctct tcgccaatat
     1321 gtaccgccat ccgagcgttg cgcgcgtgcg cgacaaggcc gagcgcctgg ttcgccagct
     1381 gttcgccgcc ttcttcgccg aaccggcgaa aatgcccgcc gaatgggcgc agttgaccgc
     1441 ggaggccgaa atgcgcgaag gcgaagcggg cagggcgcag gccgtcgccg actacatcgc
     1501 gggcatgacc gatcgctacg ccgtcgccga gcacggaagg ctctttgacg aggcgccgga
     1561 cttgcgatag agcggccggc tttccccccc aaacgagggt tcatgaatat tttcgccgat
     1621 ttccagcggc gcattgcgac gctgctcgaa gacatcgcgg cctccggcgg cttgcccaag
     1681 ccgctcgatc tcggccgctt caccgtcgag ccgccgcgcg aggcggcgca tggcgatctt
     1741 tcgaccaacg ccgccatggt cttcgccaag gaggcgaagg cggcggggtc gagcccgcgc
     1801 gctttggcga ccgagatcgc cgcgggcctc gccgagcagc cggatgtcga tcaggcggaa
     1861 gtggccgggc cgggcttcat caatatccgg ctgaagccgg tcgtcttcga aaacgtgctc
     1921 cgctcggttc tcgccgaagg gcggcggttc ggcgccggcg cggcggcggg cgagccgatc
     1981 aatgtcgaat atgtcagcgc caacccgacc gggccgatgc atgtcggcca cgcgcgcggc
     2041 gcggtgttcg gcgacgcgct cgccaacctg ctcagcttcg ccggccgcag cgtgacgcgc
     2101 gaatactaca tcaacgacgc cggcgcccag gtcgacgcgc tcgcccgctc ggcctatttg
     2161 cgctatcgcg aggcgctcgg cgaaacggtc ggggcgatcc ctgacggttt gtacccgggc
     2221 gactatctcg ttcctgtcgg cgccgcgctc gccaaggcgt tcggcaaacg ccttcaagag
     2281 aagcccgagg ccgaatggct gccgatcgtc cgccgcgagg cgatcgatgc gatgatggcg
     2341 atgatccgcg acgatcttgc ggcgctcaat gtccgccacg acgtattttc gtccgagcgc
     2401 gcgctgaccg gcgccgatgg cgggcgcgac cgcgtgcggg agacgatcga ggacctgaag
     2461 gcgcgcggca tcgtctatct cggccgtctg ccgccgccga aaggcgcgcc ggtcgaggat
     2521 tgggaggatc gcgagcagac cttgttccgc tcgaccgatt tcggcgacga cgtcgatcgg
     2581 ccgctgatca agtcggacgg cggctatacc tatttcgccg ccga
//
LOCUS       DATGJW010000252        10866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1395654, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000252 DATGJW010000000
VERSION     DATGJW010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10866
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1395654"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..121)
                     /locus_tag="VKU03_07800"
     CDS             complement(<1..121)
                     /locus_tag="VKU03_07800"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding protein"
                     /protein_id="HLJ71204.1"
                     /translation="MKRPAVHIIGAGLAGLSAAVRLSDSPASIVVHEATRQAGG"
     gene            complement(122..934)
                     /gene="hpnD"
                     /locus_tag="VKU03_07805"
     CDS             complement(122..934)
                     /gene="hpnD"
                     /locus_tag="VKU03_07805"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HLJ71205.1"
                     /translation="MNDSTGVARNSSFYTAMRILPQDKRDAMYEIYGFCRAVDDIADD
                     RGRPREERRAALQDWRRKIDALYAAGGDKGGALARAIKTYDLKREDFQAVIDGMEMDV
                     VEDIRAPDRPTFDLYCDRVACAVGRLSTQVFGLDRPTGEKLADHLGRALQTTNILRDL
                     DEDASLGRLYLPREALAKAGIAGNDPETVLAHPNLGAAAQVLVEEARWRFEQARAVMA
                     ACPRAKVRAPRLMGAVYRMILDRLVRRGFAPPRVKVSAPRLGVLFVLLRELL"
     gene            complement(931..1803)
                     /gene="hpnC"
                     /locus_tag="VKU03_07810"
     CDS             complement(931..1803)
                     /gene="hpnC"
                     /locus_tag="VKU03_07810"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010681939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HLJ71206.1"
                     /translation="MTETIPPHRTEHSENFPVASRLIAARHRGVILAFYRFVRAADDI
                     ADHSALTPEEKLARLEKMAAALNGENDDVPAGQALRAALAARDLTPRHALDLLAAFRL
                     DATKKRYADWSDLMAYCGLSAMPVGRFVLDVHGESAELWPASDAICASLQVINHLQDC
                     GEDYAGLDRVYLPEETLSRHGAAVADLGGKRASPPLRAAIVELAHRSEALLAEGASLA
                     GGVKDFRLACEIGAIVRLARANCRRLKRRDPLSERVHPSKAGFIALGLIGAIEGGFAR
                     AASHAPQPAGKRAA"
     gene            complement(1867..2874)
                     /gene="hpnA"
                     /locus_tag="VKU03_07815"
     CDS             complement(1867..2874)
                     /gene="hpnA"
                     /locus_tag="VKU03_07815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HLJ71207.1"
                     /translation="MALAIEAKDLIVVTGASGFVGSALVEALLAGPGQIRAVVRPTSA
                     RPPAPRERVEIALADMRDLDAMKEAMRGARFLFHVAADYRLWARNPQDIVAANVGGTR
                     AVMTAAAQAGVERVVYTSSVATLAPRSGAPADETCALAENQAIGAYKRSKVAAERLVE
                     AMVGQGLPAVIVNPSTPIGPRDGRPTPTGRIVIEAASGRMPAYVDTGLNLVHVDDVAK
                     GHIAALQSGRTGERYILGGDDVTLGRMLADIASLVGRPAPRISLPRAPLFPLAMVAEA
                     AARVTGHEPFLTLDGLRMAKNRMFFSSAKAERELGYRHRPYQAALKDALDWFNSNGML
                     K"
     gene            3027..3464
                     /locus_tag="VKU03_07820"
     CDS             3027..3464
                     /locus_tag="VKU03_07820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003605580.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLJ71208.1"
                     /translation="MSGVLSLWQFWAVLSAVFAALTAIFGKVGVEKVGADLATFIRTI
                     VILVFAALIVTASGQWRDAGAISSRSAVFLVMSGLATGASWLCYYRALQIGDAARVAP
                     IDKLSVVLVALFGVAFLGERLAAPNWLGVALIAAGAILVAYRA"
     gene            3588..4949
                     /locus_tag="VKU03_07825"
     CDS             3588..4949
                     /locus_tag="VKU03_07825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71209.1"
                     /translation="MTAHEKIAAPGVATGLLPPEVLAANFADLHPPLTEHEAHVEADR
                     CYFCYDAPCQKACPTSIDIPLFIREIAAGNPLGAAKTILDANIMGGMCARVCPTETLC
                     QEACVREAAEGKPVQIGLLQRYATDAIMATGRNPYTRAAPTGKRVAIVGAGPASLACA
                     HALAERGHDVTIFEAREKPAGLNEYGIAAYKTPDDFAQKEAAFILSIGGVAVKRGVAL
                     GRDIDLKSLQRDYDAVFLGLGLAGVNKLGLANEATLANVIDAVEYIAALRQAKDLSTL
                     PVGRRVVVIGGGMTAIDVAVQSKRLGAELVTIVYRRGQEHMKASAYEQELAQTNGVLI
                     RTWAKPVALEGGGAVSGVVFEKTREESGRLIGTGETFRLDADIVFTAIGQTMTPAAYG
                     GAEVLQGKDGRILVDEQRRTSLKGVWAGGDCVYGGQDLTVAAVEDGKIAARSIDAALA
                     AKG"
     gene            5160..6470
                     /gene="preA"
                     /locus_tag="VKU03_07830"
     CDS             5160..6470
                     /gene="preA"
                     /locus_tag="VKU03_07830"
                     /EC_number="1.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017113 - dihydropyrimidine dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0006210 - thymine catabolic process
                     [Evidence IEA];
                     GO_process: GO:0006212 - uracil catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent dihydropyrimidine dehydrogenase
                     subunit PreA"
                     /protein_id="HLJ71210.1"
                     /translation="MVDLRTEFLGVKSPNPFWLASAPPTDRKTNVERAFRAGWGGVVW
                     KTLGEAGPPIVNVSGARYGAIHGPDRRVIGINNIELITDRDLEINLVEIKEVKRKWPD
                     RALIVSLMVPCDEDSWKNILKRVEETECDGVELNFGCPHGMSERGMGSAVGQVPEYIE
                     MVARWCKAHTRMPVIVKLTPNITDIRFPARAALKGGADAVSLINTINSIMAVDLDAMA
                     PSPHIDGKGTHGGYCGPAVKPIALSMVSEIARDPATRGLPISAIGGVTTWRDAAEFIA
                     LGAGTVQVCTAAMTYGFKIVEEMISGLKNFMAAKGYQRIDDFRGRAVGSLVDWQYLNL
                     NYVAKAEINQDLCIKCGRCHIVCEDTSHQAIWPMVDGKRKFVVNEAECVGCNLCVIAC
                     PVENCITLVHKTQGIDPRTGLDYGRPPADWTTHPNNPAVRTAAE"
     gene            6682..7071
                     /locus_tag="VKU03_07835"
     CDS             6682..7071
                     /locus_tag="VKU03_07835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71211.1"
                     /translation="MIKAGSKRAAVAAALAVTLAVVGSAPSPAAAASAKHNKKPQAKA
                     DSGPTVAVTITNKRKSGVVELDVALAGSPAFKPLLKNLAPGKQAVVTLAHDENCAFDF
                     YVKYDDGEISTVPNVHVCDDGKLNLVE"
     gene            complement(7391..7900)
                     /locus_tag="VKU03_07840"
     CDS             complement(7391..7900)
                     /locus_tag="VKU03_07840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71212.1"
                     /translation="MAASRVIACFLFVSTVFGASSVFAAANGCDSGRWPVSPAETKFN
                     AALPTLASGDSLPALGAPVMVNLAPQSQVNFAHAPARQGKANPAYAAIVKLGPEPAAT
                     YQVIASTGAWIDIAENSDLTQSAGYERLKDCPGVNKVVRFRTAGGPLTVQISGSYGKT
                     IKIAVERVE"
     gene            complement(8060..8698)
                     /locus_tag="VKU03_07845"
     CDS             complement(8060..8698)
                     /locus_tag="VKU03_07845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019966336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator C-terminal
                     domain-containing protein"
                     /protein_id="HLJ71213.1"
                     /translation="MDRPLIKVSIRAENERVILEAAEAVFAEAGFKGATTAAIAARAG
                     VPKANLHYYFSTKEDLYRAVVERVLTAWLAAASSFDESDDPCEALARYIGAKMDLARS
                     MPLSSRIWAAEIMRGAPAIQDFLDTTLAQWVAERSRIVRRWIAEGKMRPIEPKYLFYM
                     IWATTQHYANAAHEMVSLEHGQALTDGQFEAAKRQVIETIIAGVAPQPALSV"
     gene            8867..10114
                     /locus_tag="VKU03_07850"
     CDS             8867..10114
                     /locus_tag="VKU03_07850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Zn-dependent hydrolase"
                     /protein_id="HLJ71214.1"
                     /translation="MSDLSNVRINGQRLWDALMEMAKIGATPKGGCKRLTLTDLDKQA
                     RQLFASWCEKEGCAVKVDEMGNMFARRPGAEDHLPPVMIGSHLDTQPTGGKYDGVLGV
                     LGALEVVRSLNDLKIKTRHPVEIANWTNEEGSRYAPAMISSGVFAGVYTKEFAYARED
                     AEKKKLGDELERIGFKGSEPVGGRPIHAFFELHIEQGPILEDESIDVGVVTHGQGQRW
                     YEIKLVGFESHAGSTPMPRRKDALLGAARIVELVNKIGLSKAPLGVSTVGMLNPYPNS
                     RNVIPGEVFMTCEFRHPIDETLSDMDAELKTGIEEICRKIGLTYELKQVFYYAPVAFD
                     QGCVAAVRRAAKHLGLSHRDIVSGAGHDACYIARVAPTSMIFTPCVDGVSHNESEEIS
                     QEWATAGANVLMHAVLEKAEIVA"
     gene            10257..>10866
                     /locus_tag="VKU03_07855"
     CDS             10257..>10866
                     /locus_tag="VKU03_07855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLJ71215.1"
                     /translation="MSLVIKDGTVVAADRTYKADVLVEGEKIAAIGPNLKGDTTLDGT
                     DCFVMPGGIDPHTHLEMPFMGTTSADHYESGTKAALSGGTTMVVDFCIPGNGQPLMEA
                     WAEWDKKSKTACADYAYHMCITYWSDKVREEMGKVVEKGVTTFKHFMAYKGALMVNDE
                     EMFASFQRCAELGAMPLVHAENGDVVAMLQQKYMKAGVTGPEG"
BASE COUNT         1944 a         3489 c         3608 g         1825 t
ORIGIN      
        1 gccctccggc ttggcgcgtc gcctcgtgaa cgacgatcga agcaggactg tccgacagcc
       61 tgaccgcggc cgaaaggccg gcaagtcccg ccccgatgat gtgaacggcc ggccgtttca
      121 tctagagcag ctcgcgcaaa aggacgaaga gcacgccgag gcggggcgcg ctgaccttga
      181 cgcgcggcgg cgcgaagccg cggcggacga ggcgatcgag gatcatgcgg tagacggcgc
      241 ccatcaagcg gggcgcgcgc actttggcgc gcggacaggc ggccatgacc gcgcgcgctt
      301 gctcgaagcg ccagcgcgcc tcctcgacca gaacctgcgc ggcggcgccg agattgggat
      361 gagcgaggac cgtttcggga tcgttgccgg cgatcccggc cttggccagc gcctcgcgcg
      421 gcagatagag ccggccaagg ctcgcatcct cgtcgaggtc gcgcaagata ttggtcgtct
      481 gcaaggcgcg gccgagatga tcggcgagct tttcgccggt cggccggtca aggccgaaga
      541 cctgcgtcga caggcgcccg accgcgcagg cgacgcggtc gcaatagagg tcgaaggtcg
      601 gccgatccgg cgcgcggata tcctcgacga catccatctc catgccgtcg atgaccgcct
      661 ggaaatcctc gcgcttgagg tcataggtct tgatcgctcg ggcgagcgcg ccgcctttgt
      721 cgccgccggc cgcatagagc gcgtcgattt ttctccgcca atcctgcaac gccgcgcgcc
      781 gttcttcgcg cggacggccg cgatcgtcgg caatgtcgtc gacggcgcgg caaaagccgt
      841 aaatctcgta catcgcgtcc cgcttgtctt gcggcaggat gcgcatcgcg gtgtagaagg
      901 agctgtttcg cgcgacgccg gtggaatcgt tcatgccgcg cgtttcccgg cgggttgagg
      961 cgcgtgcgag gccgcgcggg cgaagccgcc ctcgatcgcg ccgatcaggc cgagcgcgat
     1021 gaagcctgct ttgctgggat gaacgcgctc gctcagagga tcgcggcgct tgagccggcg
     1081 gcaattggcg cgggcgagcc gaacgatggc gccgatctcg caggcgaggc ggaaatcctt
     1141 gacgccgccg gcgagcgagg cgccctcggc gagcagcgct tccgaacggt gcgcgagttc
     1201 gacaatcgca gcgcgcaagg gcggcgaagc gcgcttcccg ccgagatcgg ccactgccgc
     1261 gccgtgacgg ctgagcgtct cctccggcag atagacgcgg tcgagcccgg catagtcctc
     1321 gccgcaatcc tgcaaatggt tgatcacttg cagactcgca caaatcgcgt cgctcgccgg
     1381 ccacaattcg gcgctttcgc catgaacgtc aagcacgaag cgcccgaccg gcatggccga
     1441 gagcccgcaa taggccataa ggtcgctcca atcggcatag cgtttcttcg tcgcgtcaag
     1501 acggaaggcg gcaagaagat caagcgcgtg gcgcggcgtc aaatcgcgcg ccgccagagc
     1561 ggcgcgcaaa gcctgtccgg cgggaacatc gtcgttctcg ccattgagcg ccgccgccat
     1621 tttctcgaga cgcgcgagct tctcttccgg cgtcaaggcc gaatgatcgg cgatatcgtc
     1681 ggccgcgcgc acaaagcgat agaaggcgag aatcacgccg cgatggcgcg ccgcgatcag
     1741 ccgcgaggcg acgggaaagt tctcgctgtg ttcggtccgg tgcggcggaa tggtttcggt
     1801 cacgacgttt cgccaattgc gagataatcc ctcgccccgc gaagcaggga gaagggaaga
     1861 ggagcgctac tttagcatcc cgttgctgtt gaaccaatcg agcgcgtcct ttagcgctgc
     1921 ttggtacgga cggtggcgat agccaagctc gcgctccgcc ttggcggagg agaagaacat
     1981 cctgttcttg gccatgcgca atccgtccaa tgtcaagaag ggctcgtgac cggtcacgcg
     2041 cgccgccgcc tcggcgacca tggcgagcgg gaagagcggc gcgcgcggca agctgatgcg
     2101 cggcgcgggc cggccgacga gggacgcgat gtcggcgagc atccgcccga gcgtcacgtc
     2161 gtcgccgccg agaatgtagc gctcgccggt ccggcccgat tgcagcgccg cgatatggcc
     2221 cttggcgaca tcgtcgacat ggacgagatt gaggccagtg tcgacataag ccggcatgcg
     2281 gccggaagcg gcttcgatca cgatgcggcc ggtcggcgtc ggccggccgt cgcgcgggcc
     2341 gatcggcgtc gagggattga cgatcaccgc cggcaggcct tgcccgacca tcgcctcgac
     2401 cagcctttcg gcggcgacct tgctccgctt ataggcgccg atcgcctggt tttccgccag
     2461 cgcgcaagtc tcgtccgccg gcgcgccgct tctcggcgcg agcgttgcga cgctgctcgt
     2521 atagacgacg cgctcgacgc ccgcttgcgc cgccgccgtc atcacggcgc gcgttccccc
     2581 gacattggcc gcgacgatgt cctgcggatt gcgcgcccag aggcgataat cggcggcgac
     2641 atggaagaga aagcgcgcgc cgcgcatcgc ttctttcatc gcgtcgagat cgcgcatatc
     2701 ggcaagcgcg atctcgacgc gctcgcgcgg cgcgggcggc ctggcgctgg tcggccggac
     2761 cacggcgcgg atttgccccg gtcccgcgag cagcgcctcg acaagcgccg agccgacaaa
     2821 gcccgaagcg ccggtgacga cgatcaggtc tttcgcctcg atagcgagcg ccatcccaac
     2881 ctcgaaaatg agcggcgcgg cgcaccccct tctccccgct gcgcggggcg aggggaacgc
     2941 cgcgcctccg aacgcgccgc tcgccgcctt ggcggcgacc cgagcggcgt gattaatcga
     3001 ctttgcagag caaagcgagg ttggcgatga gcggcgttct ttccctttgg caattctggg
     3061 ccgtgctttc ggccgtcttc gccgccttga ccgcgatatt cggcaaggtc ggcgtcgaga
     3121 aggtcggggc cgacctcgcc accttcatcc gcacgatcgt gatcctcgtc ttcgccgccc
     3181 tgatcgtgac ggcgagcggc caatggcgcg acgccggcgc gatttcttcg cgcagcgcgg
     3241 ttttcctcgt aatgtccggc ctcgccaccg gcgcctcgtg gctctgctac tatcgcgcgc
     3301 tgcagatcgg cgacgccgcc cgggtcgcgc cgatcgacaa gctcagtgtg gtgctggtcg
     3361 cgctgttcgg cgtcgctttt ctcggcgagc gcctcgccgc gcccaactgg ctgggcgtcg
     3421 cgctgatcgc cgctggcgcc atattggtgg cgtatcgcgc ctgacgccgc ccgatcgcgc
     3481 gctgctcacg gataatcttg acaagttcta acttgcgggc gagcatcttg accgtttggt
     3541 caggatcgtc gcgccgccaa gcgcgacgcg gcggttaggg ggttgtcatg accgcgcatg
     3601 aaaagatcgc cgcgccgggc gttgcgaccg gattgctgcc gccggaagtc ctcgccgcca
     3661 atttcgccga tcttcacccg cctctgaccg agcatgaggc gcatgtcgag gccgaccgct
     3721 gctatttctg ctacgacgcg ccctgccaga aagcctgccc gacctcgatc gacattccgc
     3781 ttttcattcg cgagatcgcc gcgggcaatc cgctcggcgc ggccaagacg attctcgacg
     3841 ccaacatcat gggcgggatg tgcgcccggg tttgcccgac agaaacgctg tgccaggagg
     3901 cttgcgtgcg cgaggcggcc gagggcaagc cggtgcagat cggcttgctg cagcgctacg
     3961 cgaccgacgc gatcatggcg acggggcgga atccctatac ccgcgccgcg ccgacgggaa
     4021 agcgcgtcgc gattgtcggc gccgggccgg cgagccttgc ttgcgcccac gcgcttgccg
     4081 agcgcggcca tgacgtgacg attttcgagg cgcgcgaaaa gccggccggc ctcaacgaat
     4141 acggcatcgc ggcctataag acgccggacg atttcgcgca gaaggaagcc gctttcatcc
     4201 tgtcgatcgg cggcgtcgcg gtgaagcgcg gcgtggcgct cggccgcgac atcgacctga
     4261 aaagcctgca gcgcgattac gacgcggtgt ttctcggcct tggcctggcc ggcgtcaaca
     4321 agctcggcct cgccaacgag gcgacgctcg ccaatgtgat cgacgcggtc gagtacatcg
     4381 ccgcgctgcg ccaggccaag gacctctcga cattgccggt cggccggcgc gtcgtggtga
     4441 tcggcggcgg catgacggcg atcgacgtcg ccgttcagtc caagcggctc ggcgccgaat
     4501 tggtgacgat cgtctatcgc cgcggccagg agcacatgaa agcgagcgcc tacgagcagg
     4561 agttggcgca gaccaacggc gtgctgattc gcacctgggc caagccggtc gcgctggaag
     4621 ggggcggcgc ggtgagcggc gtggtctttg agaagacgcg cgaggaaagc gggcggctga
     4681 tcgggaccgg cgaaaccttc cggctcgacg ccgacatcgt cttcaccgcg atcggccaga
     4741 cgatgacgcc cgccgcctat ggcggggctg aagtcttgca aggcaaggac ggccgcattc
     4801 tcgtcgacga gcagcgccgc acgagcctca agggcgtctg ggctggcggc gattgcgtct
     4861 atggcggcca ggacttgacg gtcgcggccg tcgaggacgg caagatcgcc gcgcgctcga
     4921 tcgacgcggc gctggcggcg aaggggtgag ccccgtgttt gaggctgggc tgatccctcc
     4981 catcgagcgc gcacgaacgg actttggccg acgtcgcgcc gctcgcttgt cctcaatgtc
     5041 attcccgcga aagcgggaaa tccgcgtggg gcggcctaca cgtgcggttc tctggagtcc
     5101 cgcttgcgcg ggaatgacac gcacgacttc cccagctctc tccgatgaag gaggcgctca
     5161 tggtcgatct gcgcactgag ttcctgggcg tcaaatcgcc caatccgttc tggctcgcct
     5221 cggcgccgcc gaccgaccgt aagaccaatg tcgagcgcgc cttccgcgcc ggctggggcg
     5281 gcgtggtctg gaagacgctg ggcgaggccg gcccgccgat cgtcaatgtg tcgggcgcgc
     5341 gctacggcgc tatccacggg ccggaccgcc gcgtcatcgg catcaacaat atcgaattga
     5401 tcaccgatcg cgatctcgaa atcaatctcg tcgagatcaa ggaagtcaag cggaaatggc
     5461 cggaccgcgc gctgatcgtt tcgctgatgg tgccgtgcga cgaggattcc tggaagaaca
     5521 tccttaagcg cgtcgaggag acggaatgcg acggcgtcga attgaatttc ggctgtccgc
     5581 acggcatgtc cgagcgaggc atgggctcgg cggtgggcca ggtgccggaa tatatcgaaa
     5641 tggtggcgcg ctggtgcaaa gcccatacgc gcatgccggt gatcgtcaag ctgacgccga
     5701 acatcaccga tattcgtttt ccggcgcgcg ccgcgctgaa aggcggcgcc gacgcggtct
     5761 cgctgatcaa cacgatcaat tcgatcatgg cggtcgatct cgacgcgatg gcgccttcgc
     5821 cgcatatcga cggcaaggga acgcatggcg gctattgcgg cccggcggtc aagccgatcg
     5881 cgctgtcgat ggtctcggaa atcgcgcgcg acccggcgac gcggggcttg ccgatctcgg
     5941 cgatcggcgg cgtcaccact tggcgcgacg cggccgaatt cattgcgctg ggggccggca
     6001 cggtgcaagt ctgcacggcg gcgatgactt atggattcaa gatcgtcgag gagatgatct
     6061 cgggcctgaa gaacttcatg gccgccaaag gctaccagcg gatcgacgac ttccgcggcc
     6121 gcgctgtcgg cagccttgtc gattggcaat atctcaacct caattacgtc gccaaggccg
     6181 agatcaacca ggatctctgc atcaaatgcg ggcgctgcca catcgtttgc gaggatacgt
     6241 cgcatcaggc gatctggccg atggtcgacg gcaagcgcaa attcgtcgtc aacgaggccg
     6301 aatgcgtcgg ctgcaatctt tgcgtcatcg cctgtccggt cgaaaattgc atcaccctcg
     6361 tccataagac gcaagggatc gacccgcgca ccggcctcga ttacggccgg ccgccggcgg
     6421 attggaccac ccatccgaac aatcccgcgg tccggaccgc ggcggaatag gattccgcga
     6481 acgcctttcg taacattcgc ttctcttcgt gctcttcgtg ttaaaattca ccacgaagat
     6541 cacgaaggcg atcgatgtct cgccatcccg ctatgtcgaa aaagtcgcgc gacgcagcca
     6601 ttgcggcccg ccggcgcgcc gcttccacgc ccgcgcgatt gatggcatag agagatgggg
     6661 ccgcggggca agggaggcct tatgatcaag gcaggttcga aacgcgccgc cgtggcggcg
     6721 gcgctcgcgg tcacgctggc cgtcgtcggg tcggctccaa gccccgcagc cgcggcttcc
     6781 gccaagcaca acaagaagcc gcaagccaag gccgattccg gcccaaccgt cgccgtcacc
     6841 atcacgaaca agcgcaaatc cggcgtcgtc gagctcgacg tcgcgctggc cggctcgccc
     6901 gccttcaagc cgcttctcaa aaatctcgcg ccgggcaaac aggcggtcgt caccttggcg
     6961 catgacgaga actgcgcctt cgatttctac gtaaaatatg acgatggcga gatcagcacc
     7021 gtccccaacg tccatgtttg cgatgacggc aagctgaacc tcgtcgagtg aggcggcgag
     7081 tggccgtacc cttcacgctg cgtcgggccg ttctgggaaa cgaaaccggc gccaattggc
     7141 ggaggtaagc tccggtgttc atttgggcga atggctgggg cttgtcccgg ccattcgtgc
     7201 gcgcaaactc tcgaaacctg tgccggcgga gggacggcga tcgccgggac aagcgcggcc
     7261 acgacgagtg aggcgcctgg gcggcgcgag acattgcaac cgcctccgct cggcgcaaac
     7321 gccggcatga cgcatagggc cgcgacccac tctaccctgc aagttgcggg gggaaggggc
     7381 gttacgtacc ctactccacc cgctcgacgg cgatcttgat cgttttgccg taggagccgc
     7441 tgatctgtac ggtcagcggc ccgccggcgg ttctgaaccg cacgaccttg ttgacgccgg
     7501 gacagtcctt gagccgctca tagccggccg actgggtcag atcgctgttc tcggcaatgt
     7561 cgatccaggc gccggtcgag gcgatcacct gataagtggc ggcgggctcc ggcccgagct
     7621 taacgatcgc ggcataggcg ggattggcct tgccctgccg ggcgggcgca tgggcgaaat
     7681 tcacttggct ctgcggcgcc agattgacca tgaccggcgc gcccagcgcc ggaagcgaat
     7741 cgccgctggc gagcgtcggc aaagccgcgt tgaacttagt ctcggcgggc gagaccggcc
     7801 agcgcccgga atcgcagcca ttggcggcgg cgaagacgga cgaggcgcca aagacggtcg
     7861 aaacgaaaag aaagcaggcg ataacgcgag atgcggccat gatcccctcc ctgaagaaaa
     7921 ccgttccagg tcgggaacaa acatggcaca aagagagatc aaagtcgagt caaaattcaa
     7981 cccgagcgcg caaatttttt ctctgcacgc cgccagcgcg tcgagatcgc gcggcgtcgc
     8041 ggcaaaaacc ctcgatcatt caaacggaaa gagccggctg cggcgcgacg ccggcgataa
     8101 tcgtctcgat cacctgccgc ttggccgcct cgaactggcc gtcggtcagc gcctgaccgt
     8161 gctccagcga aaccatttcg tgggcggcgt tggcgtagtg ttgggtcgtc gcccaaatca
     8221 tgtagaagag atatttgggc tcgatcggcc gcatcttgcc ctccgcgatc cagcgacgga
     8281 cgatccgcga tcgctcggcg acccattgcg ccagcgtcgt atcgagaaaa tcttgaatcg
     8341 ccggcgcgcc gcgcatgatc tcggccgccc agatgcgcga ggagagcggc atcgagcggg
     8401 cgaggtccat tttcgcgccg atatagcgcg ccaaggcctc gcaaggatcg tcgctctcgt
     8461 cgaaggacga ggcggccgcg agccaggcgg tgaggacccg ctcgaccacc gcgcgataga
     8521 gatcctcctt ggtcgagaaa tagtagtgga gattggcctt cggcacgccg gcgcgcgcgg
     8581 cgatcgccgc cgtcgtcgcg cctttgaagc cagcttcggc gaagaccgct tcggcggcct
     8641 ccaggatcac gcgctcgttc tccgcgcgaa tggagacttt gatcagcggc ctgtccatca
     8701 ggcgcgactc gtcgtcatcc gcttgctccc cgttttcgcc cttgccgggc gatcttttcc
     8761 tcggcccggc gccgcttttc ttggcgccgc gcatcaagat tgcggcattt gaccgtttgg
     8821 tcaaatttat aatagcgttg ctcccagagc ggaggggatc ggcgtcgtgt cagacctgtc
     8881 gaatgtgcgt atcaatggtc aacgcctttg ggacgcgttg atggagatgg ccaagatcgg
     8941 cgcgacgccg aagggcggct gcaagcgcct cacgctgacc gatctcgaca agcaggcccg
     9001 ccagcttttc gcctcctggt gcgaaaagga gggctgcgcg gtcaaagtcg acgaaatggg
     9061 caatatgttc gcgcgccgcc ccggcgcgga ggatcatttg ccgccggtga tgatcggcag
     9121 ccatctcgac acccagccga ccggcggcaa gtacgacggc gtgctcggcg tgctcggcgc
     9181 gctcgaagtg gtgcgctcgc tcaatgatct gaagatcaag acccgccatc cggtcgagat
     9241 cgccaactgg actaacgagg aagggtcgcg ctacgcgccg gcgatgatct cgtccggcgt
     9301 cttcgccggc gtctacacca aggaattcgc ctatgcgcga gaagacgccg agaaaaagaa
     9361 gctcggcgac gagttggagc ggatcggctt caagggctcg gagccggtcg gcggccggcc
     9421 gatccacgcc tttttcgagc tgcatatcga gcaggggcca atcctcgaag acgaatcgat
     9481 cgatgtcggc gtggtcaccc atggccaggg ccagcgctgg tacgagatca agctcgtcgg
     9541 cttcgagagc catgccggct cgacgccgat gccgcggcgc aaggacgcgc tgctcggcgc
     9601 agcgcggatc gtcgaactcg tcaacaagat tgggctgtcg aaagcgccgc tcggcgtctc
     9661 gacggtcggc atgctcaatc cctatccgaa ttcgcgcaac gtcattcccg gcgaggtctt
     9721 catgacctgc gaattccgcc atccgatcga cgagacgctg tcggacatgg acgctgagct
     9781 caagacgggg atcgaggaga tctgtaggaa aatcggcctg acctacgaat tgaagcaggt
     9841 cttctattac gccccggtcg ctttcgacca agggtgcgtc gccgcggtgc ggcgcgccgc
     9901 caagcacctt ggcctttcgc atcgcgacat cgtctccggc gccgggcacg atgcttgcta
     9961 catcgctcgc gttgcgccga cctcgatgat tttcacgccc tgcgtcgatg gggtcagcca
    10021 taacgagagc gaggagatca gccaggaatg ggcgacggcc ggcgccaatg tgctgatgca
    10081 tgcggtgctg gagaaggcgg agatcgtcgc gtgaggcgaa cggtggcgcg tttacatgga
    10141 gcccgtcatt gcgaggagcg aagcgacgaa gcaatccatt cgtaaggcgc agccccagga
    10201 acggattgct tcgctttgct cgcaatgacg gcgggctagt ctgggagatt taaatcatgt
    10261 cgctcgtcat caaggacgga acggtggtcg ccgccgaccg cacctacaaa gcggacgtgc
    10321 tcgtcgaggg agaaaagatc gcggcgatcg gccccaacct caagggcgac acgacgctgg
    10381 acggaaccga ttgcttcgtc atgccgggcg gcatcgaccc gcacacccat ctcgaaatgc
    10441 cgttcatggg cacgacctcg gcggatcatt atgagagcgg caccaaggcg gctctgtcgg
    10501 gcggcacgac catggtcgtc gacttctgca ttcccggcaa cggccagccg ctgatggagg
    10561 cgtgggccga atgggacaag aagtccaaga ccgcttgcgc cgattacgcg taccatatgt
    10621 gcatcaccta ttggtcggac aaggtgcgcg aggagatggg caaggtcgtc gagaaaggcg
    10681 tgacgacgtt caagcatttc atggcttata agggcgcgct gatggtcaat gacgaggaga
    10741 tgttcgcctc gttccagcgc tgcgccgagc tcggcgccat gccgctcgtc catgccgaga
    10801 acggcgacgt cgtcgccatg ctgcagcaaa agtatatgaa ggccggcgtc accggccccg
    10861 aaggcc
//
LOCUS       DATGJW010000253         4536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1084887, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000253 DATGJW010000000
VERSION     DATGJW010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4536
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1084887"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..250
                     /locus_tag="VKU03_07860"
     CDS             <1..250
                     /locus_tag="VKU03_07860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71216.1"
                     /translation="VDLALLDINLGQELVYPAAEILADRGVQFVFLTGYDNEHVDPGF
                     RYAPVLQKPFDREALQRALTESSAPPAEETIARKAARA"
     gene            381..596
                     /locus_tag="VKU03_07865"
     CDS             381..596
                     /locus_tag="VKU03_07865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71217.1"
                     /translation="MFSIVGLSFCCMIAGGLIGLEWTSFSGVFFSVAALAAIVFFSAI
                     DAWECEGGASESGEAPAPRALNGARSR"
     gene            656..2167
                     /gene="zwf"
                     /locus_tag="VKU03_07870"
     CDS             656..2167
                     /gene="zwf"
                     /locus_tag="VKU03_07870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004345 - glucose-6-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate dehydrogenase"
                     /protein_id="HLJ71218.1"
                     /translation="MSGPAKWPHGSAAGVNPAPPCTLVIFGAGGDLTKRLLTPALYDL
                     AVARLLDEKTTIVGVDHADLNDQTWTQSLAEALRSFTADKSAEFHAGHIDRQAWDWLS
                     RRLSYCKANFEDDAAFAALGKRLSGNVVFYLAVGARFFGPIATKLGAAGLLQEKPDAF
                     RRIIVEKPFGGDLASAEALNRVFLAAAEERQVFRIDHFLGKDMVQSILAIRFANRMFE
                     PIWRADHVDHVQITAAETVGVEERGAFYDTTGALRDMVPNHLFQLLCMAGMEAPGSFE
                     AEAVRSEKAKLVRAVRPIQPGEAARGQYAAGRLDGRSLPGYRDEPHVGKDSRTETYAA
                     LKLTIETGRWAGVPFYLRTGKRLKARKTEIALCFKPTAYDLLADKAGLAPNILRLQID
                     PEHGTRAHFNVKAPGPEMALADVATVFRYQDAFPQRPNVGYEALLYDCMIGDATLFQR
                     ADNIEAGWAAVQPLLDAWRSGAPELYPAGAEGPASADALIAKDGRAWLSLAQD"
     gene            complement(2181..2444)
                     /locus_tag="VKU03_07875"
     CDS             complement(2181..2444)
                     /locus_tag="VKU03_07875"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71219.1"
                     /translation="MRSTHARFAQGEYGPELVRLMSDALESAWLQARGAVRDAELGRL
                     VMASAIIEQVDRGVTAHEELVATARKALAAALRLSPGEEPSPA"
     gene            complement(2462..2686)
                     /locus_tag="VKU03_07880"
     CDS             complement(2462..2686)
                     /locus_tag="VKU03_07880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71220.1"
                     /translation="MEQIFGLVGSGSLFLAYCAYCEARDALQSAPSLHISPKRAKMGG
                     LTAAGITLAVGWFICLALARAGALYDPSAF"
     gene            complement(2861..3364)
                     /locus_tag="VKU03_07885"
     CDS             complement(2861..3364)
                     /locus_tag="VKU03_07885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003598253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HLJ71221.1"
                     /translation="MGLFSKDIQNLNDLFIHQLQDIFYAEKQIVKNLPTMIDKATSPG
                     LKQGLSSHLRETEGHVERLRQVFEMNEAKEKAVDCPAIDGIIEEANDVISEAAGEEVR
                     DAALTAAAQAVEHYEISRYGTLVAWAKMLGRDDCASLLEETLKEEKAADKKLTQLATD
                     QINLRAA"
     gene            complement(3482..4372)
                     /locus_tag="VKU03_07890"
     CDS             complement(3482..4372)
                     /locus_tag="VKU03_07890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71222.1"
                     /translation="MAADILDRSAAPTTKNAAPALESRRSAVSFSAIFAGALAMAASS
                     LVLAFVGAGMGLAIATPWSGAAAAATKFAASTAIALVIIEWLSAGIGGYVAGRMRVKW
                     ASVHDEQVFFEDTAHGFLAWSVATLFAAWLFAGAFAATLSGGAHALSSAATNSSRGAA
                     TASAEIASPLTGQVGYWTDELLRAPQSAAANSSAGDARGEIERILSRGLIGDIPQADR
                     DYAAQLVAAKAGVDASEAKKRVDAVVQQIGEAKHSATALADSARKVAAATSMMTALAL
                     AVGAFIASAAAALGGWRRDE"
BASE COUNT          767 a         1508 c         1479 g          782 t
ORIGIN      
        1 cgtcgatctg gccctgctcg acatcaatct cggccaggag cttgtctatc ccgcggcgga
       61 gatcctcgcc gatcgcggcg tccaattcgt gtttctgacc ggctacgaca acgagcatgt
      121 cgaccccggc ttccgctatg cgccggtgct gcaaaagccg ttcgaccgcg aagccctgca
      181 aagggcgctg accgaatcga gcgcgccgcc ggcggaagag acgatcgccc ggaaggcggc
      241 gagggcctga gccggcgccg gccgttcgga agaggcgacg aggagcgcga gaaatgaacg
      301 cgaagccgcg gaacctttcg cccgggctcg cgttctggcc tcaatcggct gggcggcgcg
      361 gtctcgtggg gcccttttcc atgttttcca tcgtcgggtt gtcgttctgc tgcatgattg
      421 ccggcggcct catcggcttg gaatggacca gtttcagcgg cgttttcttc tccgtggccg
      481 ccttggcggc gatcgtcttc ttttcagcga tcgacgcctg ggaatgcgag ggcggcgcgt
      541 ccgaatcggg agaagcgcca gcgccccggg cgctgaacgg cgcgagatcg cgctgaatgg
      601 agcgccggac tgcggctaag atcctgaaaa gcgcgccgat cgcggccggg ccgcgatgag
      661 cggtcctgcg aaatggccgc atggatcggc ggcgggcgta aacccggcgc cgccttgcac
      721 tttggtgatt ttcggcgccg gaggcgatct gaccaagcgc ctgctgacgc cggctctcta
      781 tgatcttgcc gttgcgcgcc ttctcgacga gaagacgacg atcgtcggcg tcgatcacgc
      841 cgatctcaac gaccagacct ggacgcaatc gctggccgag gcgctgcgct cattcaccgc
      901 cgacaagtcc gccgaattcc acgccggcca tatcgaccgg caagcctggg attggctcag
      961 ccggcggctc agctattgca aggcgaattt cgaggacgac gcggcctttg ccgcgctcgg
     1021 caagcggctg agcggcaatg tggttttcta tcttgctgtc ggcgcccgat ttttcggccc
     1081 gatcgcgacc aagctcggcg cggccggctt gctgcaggag aaaccggacg ccttccgccg
     1141 catcattgtc gagaagccgt ttggcggcga tctcgcctcc gccgaagccc tgaaccgggt
     1201 ctttctggcg gccgccgagg agcggcaagt cttccgcatc gatcactttc tcggcaagga
     1261 tatggtccag tcgatcttgg cgatccgctt cgccaaccgg atgttcgagc cgatctggcg
     1321 cgccgaccat gtcgaccacg tgcagatcac cgccgccgaa acggtcggcg tcgaggagcg
     1381 cggcgctttt tacgacacca ccggcgcctt gcgcgacatg gttccgaacc atctcttcca
     1441 actgctttgc atggcgggga tggaggcgcc gggaagcttc gaagccgagg cggtccgctc
     1501 ggaaaaggcg aagctggtcc gcgccgtgcg gccgatccag ccaggcgagg cggcgcgcgg
     1561 ccaatacgcc gccggccggc tcgacgggcg ctccctgccg ggctatcgcg acgagccgca
     1621 tgtcggcaag gacagccgca ccgagactta cgcggcgctc aagctgacga tcgagaccgg
     1681 ccgctgggcc ggcgttcctt tctaccttcg gaccggaaag cgcctgaagg cgcgcaagac
     1741 cgagatcgcg ctttgcttca agccgaccgc ctatgatctg ctggccgaca aggccggcct
     1801 tgcgccgaat attcttcggc tgcaaatcga tccggagcat ggcacgcgcg cccatttcaa
     1861 tgtcaaggcg cccggccccg aaatggcgct cgccgacgtc gccaccgtct ttcgctatca
     1921 ggacgcgttc ccgcagcgtc ccaatgtcgg ctatgaggcg ctgctctacg attgcatgat
     1981 cggcgacgcc acgctgtttc agcgcgccga caatatcgaa gcgggctggg ccgcggtcca
     2041 gcctcttctc gacgcctggc gctcgggcgc gccggagctt tacccggccg gcgctgaagg
     2101 tccggcaagc gccgacgcgc tgatcgccaa ggatggaagg gcgtggcttt cccttgcgca
     2161 ggactgagcg ctgggcgggc tcaggcgggc gacggctcct cgccgggcga tagccgcaaa
     2221 gcggccgcca gcgcctttcg cgcggtggcg acgagttctt catgagcggt gacgccccga
     2281 tcgacctgct cgatgatcgc gctcgccatg accaggcggc caagctccgc gtcgcggacg
     2341 gcgccccgcg cctgcagcca ggcgctctcc aaagcgtcgc tcatcaaacg gacgagttcc
     2401 gggccgtatt ccccttgcgc gaagcgcgca tgcgtcgatc gcattttatc cttcagcaaa
     2461 attaaaaagc gctgggatcg tataaagccc cggcccgggc gagcgcgagg cagatgaacc
     2521 agccgacggc cagggtgatc ccggccgcgg tcagcccgcc catcttagcc cgcttgggcg
     2581 agatgtgaag agacggcgcc gattgcagcg catcgcgcgc ctcgcaatag gcgcagtagg
     2641 cgagaaagag gctgccgctc ccgacaaggc cgaaaatctg ctccatggat cgccctctcc
     2701 tgcttcgcct cgcctgcggg gcgagccgcg gcccctttct tttcgaccca atttcgaccc
     2761 gaatgtcgcc atacaaacgc tcaaacggcg ccgaagttcc gttcggcgcc gaaggggcgg
     2821 gagatgaaag gccgcgcccg aatgtggcgc ggccgaaggc tcaggccgcg cgcaaattga
     2881 tctggtctgt ggccaattgc gtgagtttct tgtcggcggc tttctcttcc ttcaaggtct
     2941 cttcgagaag cgaggcgcaa tcgtcgcgac cgagcatttt cgcccaggcg accagcgtgc
     3001 cgtagcgcga aatttcgtaa tgctcgacgg cttgcgccgc cgccgtcagc gccgcatcgc
     3061 ggacctcttc gccggccgct tcgctgatca cgtcattcgc ctcttcgata atgccgtcaa
     3121 tcgccggaca atcgaccgcc ttctccttag cctcgttcat ttcgaacact tgccgcaggc
     3181 gctccacatg cccctcggtc tcgcggagat ggctggaaag gccctgcttc aagccaggac
     3241 tggtcgcctt gtcgatcatg gtcggcagat tcttcacgat ctgcttttca gcgtagaaga
     3301 tgtcctgaag ctggtggatg aagaggtcgt tcaggttctg gatgtctttc gaaaaaaggc
     3361 ccatggcctc gccctttccg tcacagagga agcttcgcgg acctcgcccg gtcggcgagc
     3421 cgccgccact caacgccacg ctttggggcg agttccgcgc cgcggcttcc gcgcggcgcc
     3481 gtcattcgtc gcgccgccag ccgccaagcg ccgccgcagc gctggcgatg aaagccccaa
     3541 ccgccagcgc gagcgcggtc atcatcgagg tggccgccgc gaccttgcgg gcggaatcgg
     3601 caagagctgt cgccgaatgc ttcgcctcgc cgatctgctg gacgacggcg tcgacgcgct
     3661 tcttcgcctc gctcgcatcg acgccggctt tcgcggcgac aagctgcgcg gcatagtcgc
     3721 ggtcggcttg cggaatatcg ccgatcaggc cgcggcttag aatgcgctcg atctcgccgc
     3781 gcgcgtcgcc ggcgctcgaa ttggccgccg cgctttgcgg cgcgcgcaac agctcgtccg
     3841 tccaatagcc gacttgtccg gtcaagggcg acgcgatctc agccgacgcc gtcgccgcgc
     3901 cgcggctcga attcgttgcg gcgctggaaa gagcatgcgc gccgccgctc agcgtcgcgg
     3961 cgaaagcgcc ggcgaatagc caggcggcga agagcgtggc gacgctccag gcgagaaagc
     4021 catgcgcggt gtcctcgaag aaaacctgct cgtcatggac gctcgcccat ttgacgcgca
     4081 tgcggccggc gacatagccg ccgatcccgg ccgatagcca ctcgatgatg acgagcgcga
     4141 tcgcggtgga ggcggcgaac ttcgtcgccg cggcggcggc gccgctccat ggcgtcgcga
     4201 tcgccaggcc cattcccgcg ccgacaaaag cgagcacgag agaggatgcg gccatggcga
     4261 gggcgccggc gaagatcgcg ctgaacgaaa ccgccgagcg gcgggattcg agcgccggcg
     4321 cggcgttttt cgtcgtcggg gcggcgcttc ggtcgagaat gtcggcagcc atagacgtgc
     4381 tcgctcaatg gtacagaaag gcgatcaaga tgatgatcgg aatcggcacg ccgagaagcc
     4441 agaggagaag agcgcggccc atgaaaacgt ctccgaaaga ccatgcgttg agcgcattgg
     4501 cgtcggacaa cggcgaagcc gcggcaaagt tccttt
//
LOCUS       DATGJW010000254         3205 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_255493, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000254 DATGJW010000000
VERSION     DATGJW010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3205)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3205)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3205
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_255493"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..829
                     /gene="frr"
                     /locus_tag="VKU03_07895"
                     /pseudo
     CDS             263..829
                     /gene="frr"
                     /locus_tag="VKU03_07895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407583.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
     gene            856..1626
                     /locus_tag="VKU03_07900"
     CDS             856..1626
                     /locus_tag="VKU03_07900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018320207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenyl transferase"
                     /protein_id="HLJ71223.1"
                     /translation="MMKPPIDHDPDPPISSAPIHVGIIMDGNGRWAKARGLPRFEGHR
                     RGVEAVRRAVRSAGELGLRYLTIYSFSSENWSRPAEEISDLMGLLKRFIRNDLAELHQ
                     SNVRVKVIGARGNLAPDVAALLAEAEELTRNNGGLTLVVAFNYGARQEIAEAARRLAA
                     DALAGKIDAASIDEASLSARLDTAGIPDPDLVIRTSGEQRLSNFLLWQAAYAELVFLP
                     IHWPDFDHAAFVSALAQYAARERRFGNVAPKPIVKSAS"
     gene            1634..2491
                     /locus_tag="VKU03_07905"
     CDS             1634..2491
                     /locus_tag="VKU03_07905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidate cytidylyltransferase"
                     /protein_id="HLJ71224.1"
                     /translation="MAADPQRATGDSAPPVSPRKSSELPARIAASLAMMALALAAVWA
                     GAMWFMLFWLIAFALVNWEWQGLIGGGGRALRVMLGNIVLAAAAVLAISGEARDAIIA
                     ALVGAALASIGFWRRPWPAGGVLYAAAPLIAVCVLRSSEEFGARSILWLFALVWGVDV
                     TAYFGGRLIGGPKLWPRVSPGKTWSGFIVGIACGAAISILLAPPGGAKFALFALALVG
                     GAIAQGGDLFESAIKRRFGAKDSSHLIPGHGGLMDRLDGFIAAAIFAALFGVARGGLA
                     HAARGLLLW"
     gene            2496..>3205
                     /locus_tag="VKU03_07910"
     CDS             2496..>3205
                     /locus_tag="VKU03_07910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176463.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="1-deoxy-D-xylulose-5-phosphate reductoisomerase"
                     /protein_id="HLJ71225.1"
                     /translation="MTRSIGQKEGSAESGFGAPRRLVILGATGSIGRSCADVILNAPG
                     RFEVVALVGGRDGASLAKAAIALGARYAAIADPAAYGDLKAGVAGRGVEVGAGPEAVK
                     EAALREADLVVAAIAGTVGLAPTYAAIAAGRAVALANKEPLVCAGAAVTRSARASGAR
                     LLPLDSEHNAIFQALGGRDASSAVRMTLTASGGPFRDWSLERINAATKAEALAHPNWS
                     MGPKITIDSATLMNKGLEL"
BASE COUNT          483 a         1092 c         1105 g          525 t
ORIGIN      
        1 cgttggcgga aaggacgcgg ccgaccgtga cctcggcgtc cttggcgaaa gcctgccgga
       61 tcggctgaag cagcggatcg acctcgccgc tggcggcggg cgccgcgctg gcggattttc
      121 gccccatctt catggctctc cttcatgcgg ctaaagcttt gctgcgggcg gcaaaagctt
      181 atgctatcag tcgcggctca ggcgcgacaa acgcagcgaa tcggccgcgg gccgtcgcgc
      241 ggcgaaataa aataggttga tcatggcgca gcagccagcc ttcgacattg ccgagatcaa
      301 gcgccggatg caggcggccc tgcaaacgct caagcacgag ttgggcggct taaggaccgg
      361 ccgcgcatcc gccagcctgc tcgagccggt ccatgtcgac gcctacggcc agagaatgcc
      421 gctcaatcag gtcgcgacga tcagcgtgcc cgagccgcgc gccctctccg tgcaggtctg
      481 ggacaagagc atggtggcgg cggtcgacaa agccatccgc gacgccaatc tcggcctcag
      541 cccgacggtc gaggggcaag tcctgcgcat ccgcattccc gagctcaacg agcagcgccg
      601 caaggagatg gtcaaggtgg cgcataaata cgcctaggag gcgcgcgtcg ccgtccgcca
      661 tgtccgccgc gacggcatcg acatcctcaa gcggcagttg aaggatcgtg cgatcagcga
      721 ggacgacgag aagcgccacg aagccgaagt gcagaaggcg accgatcagg cgatccacga
      781 agtcgatcag gcgctcgcca gcaaagaaaa ggaaatcatg caggtctagc gaaggatggc
      841 ctgcttgagc cgcgcatgat gaagccgccg atcgaccatg atcccgatcc gccgatttcc
      901 tcggcgccca tccatgtcgg catcatcatg gacggcaatg gccgctgggc caaggcgcgc
      961 ggcttgcctc gcttcgaggg ccatcgccgc ggcgtcgagg cggtgcgccg cgccgtgcgc
     1021 tcggccggcg agctcggcct acgttacctg accatctaca gcttctcctc cgagaactgg
     1081 agccggccgg cggaggaaat ctccgatctg atgggcctgc tcaagcgctt catccgcaac
     1141 gatcttgccg aattgcacca gagcaatgtc cgcgtgaagg tgatcggcgc gcgcggcaat
     1201 ctggcgccgg atgtcgccgc cctgctcgcc gaagccgaag agctgacgcg caacaatggc
     1261 gggctgacgc tggtcgtcgc gttcaattat ggcgcgcgcc aggagatcgc cgaggcggcg
     1321 cgccggctcg cggcggacgc gctggccggc aagatcgacg cggcttcaat cgacgaagcc
     1381 tcgttgagcg cgcggctcga cacggccggc attcccgatc ccgatctcgt catccgaacg
     1441 tcgggcgagc agcgcctgtc gaatttcctg ctctggcagg ccgcttatgc cgagttggtc
     1501 ttcctgccga tccattggcc ggatttcgat cacgcggcgt tcgtctccgc gctcgcccaa
     1561 tatgcggcgc gcgagcgccg gttcggcaac gtcgcgccga aacccatcgt caaatcggcc
     1621 tcgtgagcgc ttgatggccg ccgatccgca gcgcgcgacg ggcgacagcg cgccgcccgt
     1681 ttcgccgcgc aaatcctccg aattgccggc gcggatcgcc gcgagcttgg cgatgatggc
     1741 gctcgcgctc gccgccgtct gggccggcgc gatgtggttc atgctgttct ggctgatcgc
     1801 cttcgcgctg gtgaattggg aatggcaggg gctgatcggc ggcggcggac gggcgctgcg
     1861 cgtgatgctc ggcaatatcg tcctcgccgc ggccgccgtg ctggcgatca gcggcgaagc
     1921 ccgggacgcg atcatcgccg ccctggtcgg cgccgcgctc gcctcgatcg gcttctggcg
     1981 ccggccctgg ccggcgggcg gcgttcttta cgcggcggcg ccgctgatcg ccgtctgcgt
     2041 gttgcgctcc agcgaggaat tcggcgcccg ctcgattcta tggctgttcg ccctggtctg
     2101 gggcgtcgac gtgacggcct atttcggcgg ccggctgatc ggcgggccga aattgtggcc
     2161 acgcgtctcg ccgggcaaga cctggtccgg cttcatcgtc gggatcgcct gcggcgccgc
     2221 gatcagcatc ttgctggcgc cgccgggcgg ggcgaaattc gctctcttcg cgctcgccct
     2281 ggtcggcggg gcgatcgcgc agggcggcga tctcttcgaa tcggcgatca agcgccgttt
     2341 cggggcgaag gattcaagcc atttgattcc cggccatggc gggctcatgg acaggcttga
     2401 cgggtttatc gccgccgcga tcttcgcggc tttgttcggc gtcgcccggg gcggcctcgc
     2461 ccatgcggcg cgaggtcttt tgctatggtg agtttgtgac gcgctccatt gggcagaaag
     2521 aaggttcggc cgaaagcggc ttcggcgcgc cgcggcggct ggtcattctc ggcgcgaccg
     2581 gctcgatcgg ccgctcctgc gccgacgtca tcctcaacgc gccgggccgt ttcgaagtcg
     2641 tcgcgttggt cggcggccgc gacggcgcca gcctcgccaa ggccgcgatc gcgctcggcg
     2701 cgcgctacgc cgccatcgcc gatcctgccg cctatggcga tctcaaagcc ggcgtcgccg
     2761 ggcgcggcgt cgaggtcggc gccggcccgg aggcggtcaa ggaggcggcc ctgcgcgagg
     2821 ccgaccttgt cgtcgcggcg atcgccggca cggtcgggct cgccccgacc tacgccgcca
     2881 tcgccgccgg gcgcgccgtg gcgctcgcca acaaggagcc gctggtctgc gccggcgccg
     2941 cggtgacgcg ctccgcgcgc gcgtccgggg cgcggctgct gccgctcgat tccgagcaca
     3001 acgcgatttt ccaggcgctg ggcggccgcg acgcttcgag cgccgtgcgg atgacgctca
     3061 ccgcctcagg cggcccgttc cgcgactgga gcctcgaacg catcaacgcc gccaccaagg
     3121 ccgaggcgct ggcccatccc aactggtcta tggggccgaa aatcaccatc gattcggcga
     3181 cgctgatgaa caagggcctg gagct
//
LOCUS       DATGJW010000255        10692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_462198, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000255 DATGJW010000000
VERSION     DATGJW010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10692
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_462198"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..354
                     /locus_tag="VKU03_07915"
     CDS             <1..354
                     /locus_tag="VKU03_07915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71226.1"
                     /translation="LRERAGVRASHPALADLLAAAHPHPALRATFSRKWEKGLGRCAA
                     YVDTAFASANERRIAPLSRLRERAGVRASDAEVQSFSPPRTLIQRVVPFSRKREKDVA
                     AVGDRGYSAPPANLG"
     gene            404..1546
                     /locus_tag="VKU03_07920"
     CDS             404..1546
                     /locus_tag="VKU03_07920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative peptidoglycan glycosyltransferase FtsW"
                     /protein_id="HLJ71227.1"
                     /translation="MPSRAERGLFADWFRTVDRWLLGCFGALMVIGLVMTLAASPAVA
                     ERLNLPTFHFVNRQAALLLPSALLMIATSFLSPRYVRRAALIVFVVSLALIILALMFG
                     VEIKGSRRWIFGVQPSEFLKPAFVVLAAWAFSEGANGRTKFAGVGIALLLLPIAIGPL
                     IMQPDFGQTMLISLVWAALFFMAGLHWFWVAGVGGVGAVGAMLAFRFSPHVHERVLRF
                     IDPDSSGGLVDTFQVDTALQSILSGGWLGRGPGEGVYKRILPDAHTDFVFAVTGEEFG
                     MIACLALIAFFGFIVLRGLLLAARNEDPFCRFAAAGLVIMFGVQSWINMAVNLRAIPA
                     KGMTLPFISYGGSSILALALGMGFLIALTRKRPRAALTAASLAEAP"
     gene            1543..2646
                     /locus_tag="VKU03_07925"
     CDS             1543..2646
                     /locus_tag="VKU03_07925"
                     /EC_number="2.4.1.227"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0050511 -
                     undecaprenyldiphospho-muramoylpentapeptide
                     beta-N-acetylglucosaminyltransferase activity [Evidence
                     IEA];
                     GO_function: GO:0051991 -
                     UDP-N-acetyl-D-glucosamine:N-acetylmuramoyl-L-alanyl-D-
                     glutamyl-meso-2,
                     6-diaminopimelyl-D-alanyl-D-alanine-diphosphoundecaprenol
                     4-beta-N-acetylglucosaminlytransferase activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0007049 - cell cycle [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0030259 - lipid glycosylation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine--N-acetylmuramyl-
                     (pentapeptide) pyrophosphoryl-undecaprenol
                     N-acetylglucosamine transferase"
                     /protein_id="HLJ71228.1"
                     /translation="MSRARAILIAAGGTGGHLFPAQALAAALAARGVPAELATDERAL
                     KYGGDFPARAIHAIPSATPTGAGIAAKARATLTLARGTFVARALIRKIAPLAVIGFGG
                     YPTVPPLLAAAWLGLPTLLHEQNAVMGRANRFLAGRVGLVATGFPALKGVDDKIRAKA
                     RYTGNPVRPAVIAAAATPFPDFADGRLRLLVTGGSQGARVMADVVPAALALLSEGERR
                     RLRLTQQARGEDKQRVAQACAALDFPVELAEFFSDLPDRIAAAHLVIGRAGASTVSEL
                     AVIGRPSILVPFPHALDQDQAANAATLEAAGAAEVVRQREFTPGRLAASLRKALADSA
                     PLAAAAAAAKAAGVADAAERLADLVVTAAGIKS"
     gene            2745..3581
                     /locus_tag="VKU03_07930"
     CDS             2745..3581
                     /locus_tag="VKU03_07930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011315903.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="folate-binding protein"
                     /protein_id="HLJ71229.1"
                     /translation="MANWTLLPDRSVLSVLGEDAAGFLHGLVTSDVESLAAGQARFAA
                     LLTPQGKILFDFFIYRAPEETGAAFFIDCPSQNAGDLAKRLGFYRLRAKVTIADLSAD
                     KAVAVCWTDPPQAANGLVFADPRDARLGFRAILPRAAAPALDQARLDQYEAHRIAIGA
                     PKGGVDFAYGDAFPHDVNMDLLHGVDFDKGCYVGQEVVSRMRHRGGVRKRIVRVKLAQ
                     SAPGAGTPILAGESPIGALGSTAGGEGLALLRIDRLAEAEQAGRPLTANGVAVVAECA
                     PE"
     gene            3652..4947
                     /gene="ribB"
                     /locus_tag="VKU03_07935"
     CDS             3652..4947
                     /gene="ribB"
                     /locus_tag="VKU03_07935"
                     /EC_number="4.1.99.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008686 -
                     3,4-dihydroxy-2-butanone-4-phosphate synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3,4-dihydroxy-2-butanone-4-phosphate synthase"
                     /protein_id="HLJ71230.1"
                     /translation="MKLSDWLTRHNVKRADFARRIGVSPGAITQICRDEGAWISRETA
                     ELIFKETRGAVTPNDFLGAVFSPNQESEMSHSVTEAVEALAAGEIVIVTDDDDRENEG
                     DLICAATLCTTEKMAFIIRYGCGIVCAPLTTQEAQRLHLSPMVAMNEAPLGTAFTVSV
                     DVKHGLTTGISAEQRANTARALANRNMAASDFARPGHVFPLIAKEGGVLMRSGHTEAA
                     VDLCKLAGLAPVAVICELANDDGTVMVGPQIEAFAQKHNLKRISVADLIAYRQAREKL
                     VERVASFPVKSEIGELAGYAYRTPFDSVLNFAFVYGPIGDGRDVPARLHRTDIVSDIF
                     GGGVIPRTLKRFKQEGRGVLVYLRDGSAGVPTNLTTTDPAASSENVRATQWREIGLGA
                     QILRDLGVSSIRLRTENPRKYVGLSGFGIEISAVEGLDC"
     gene            complement(4969..5538)
                     /locus_tag="VKU03_07940"
     CDS             complement(4969..5538)
                     /locus_tag="VKU03_07940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015686592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase"
                     /protein_id="HLJ71231.1"
                     /translation="MPGPFRILGYAIVSSDGMIADSTGLMPNALIFGADKDFFERELA
                     RADAVVQGRNSYEYQADSPNRRRLVLTRQIASLAADPNFPRGFLWNPAGASFAEACLK
                     LGLEKGTIAILGGPYVYKLFLDLGFDEFYLCRAARVALPGGVTVFPGVGPGRTPEDVL
                     KEYGLEPGPVQTLDEANALTFVVWTRRSA"
     gene            5814..6545
                     /locus_tag="VKU03_07945"
     CDS             5814..6545
                     /locus_tag="VKU03_07945"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLJ71232.1"
                     /translation="MSTDYVSENLSLPDLSRHFPNMIAGDKKSATWPYFRKEIDHTWY
                     VDRRDPQIGFINKDEAAILYANARLFAGKNGLEIGAWRGWSSCHLLAAGLATLHIVEP
                     KLADPDWNREFAAAVTAVGGGERAIFVPGLSPAEVVRLGESGQRWSFAFIDGDHDGDA
                     PKNDALATIRFLEPTAMVLFHDLASPHVAAALHALKAQGWRTLIYQTAQIMGAAWRGE
                     VAPAAHKPDPRQTWRLPDHLAGLAT"
     gene            complement(6570..7289)
                     /locus_tag="VKU03_07950"
     CDS             complement(6570..7289)
                     /locus_tag="VKU03_07950"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF017026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HemK2/MTQ2 family protein methyltransferase"
                     /protein_id="HLJ71233.1"
                     /translation="MLEVRRKEIARTSVLGKLGLSPLVPNTAVDISASHHAHVVEQNV
                     PAHFLIDGLSIDAPAGVYHPTPNSSSQLFLRNLKAMDKNKIRKILEIGAGCGAISLYL
                     AKHWGAEVVASDISPVAVESIRRNARANDVALKTVQSDLFEKIEERGFDLIIFNAPLI
                     DKEPESSVERYSLCDPGGQITRAYLREAGRFLKKSGMAIVSISSNSAYEALDDIDLRL
                     KVIGFELSYSGFWWAIVSAEL"
     gene            7596..7907
                     /locus_tag="VKU03_07955"
     CDS             7596..7907
                     /locus_tag="VKU03_07955"
                     /EC_number="4.2.1.96"
                     /inference="COORDINATES: protein motif:HMM:NF002017.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008124 -
                     4-alpha-hydroxytetrahydrobiopterin dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006729 - tetrahydrobiopterin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4a-hydroxytetrahydrobiopterin dehydratase"
                     /protein_id="HLJ71234.1"
                     /translation="MAKYKILSEADVATEVAKLKDWSVKSPNIEASFDFKTFRDAISF
                     IVQVAIEAEVMNHHPEFHNSYNKVSFSFCTHDAGFKITDLDIETASRISALARRFQSM
                     A"
     gene            7917..8735
                     /locus_tag="VKU03_07960"
     CDS             7917..8735
                     /locus_tag="VKU03_07960"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLJ71235.1"
                     /translation="MLDVQKKDKAGATPAAEAEGKPPLLPVGAWQTAGRNLLPGKPIS
                     PADMPDYTVKRDDLAEIYAAAFLRRLKALNAFVKQAGERLEGGIFYWHGDAAYHDSPP
                     AADLAPARRNLWRAARFKRSMLEIGVNAGHSALLALSANPRLVYTGIDLGVHRYTEQC
                     VAYLAAEFPGRVRYLQGDSRDVLPQLAARRDAWDFDLYHVDGGHTDDVCRTDVGNCLT
                     IAEGGRGRHLLLDDINASWIFDVYCEFVAAGRLATETFFGDWEDVNRNVLARIL"
     gene            8786..9625
                     /locus_tag="VKU03_07965"
     CDS             8786..9625
                     /locus_tag="VKU03_07965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71236.1"
                     /translation="MSDSQFATFWRGPLNPIAYSCLASFPSVGASLSVYAYEGDLELP
                     PGVERADARRICPDPSLLHRYRVNGKPSIAMFADMFRYKLVRDTGCCWVDADLVCLRR
                     PDFSGEAIVYGRQAEARGKALINNAVLKLPPDHPLLTDLIQRAEAAVDIDQSWGAIGP
                     FLLTDLAEKHGVDHLARDETEFYPIDADRFWLPLLPATSEAAAKATSGATFLHLWSAM
                     FERSHYDLSSCPPPGSFLHDAFKRLGTLHRFPRVYDEPMLSRLLSEWIAKGAPSSAAL
                     ASS"
     gene            9682..10335
                     /locus_tag="VKU03_07970"
     CDS             9682..10335
                     /locus_tag="VKU03_07970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLJ71237.1"
                     /translation="MSELKRIFERIAARNKWRNRESRSGDGSTLFYTRHLRSRLETFV
                     REFDIRTFFDAPCGDFNWMRAVAFPPRVTYIGGDIALALIKENRKRYSEPRRRFLQFD
                     IVSDPFPAADVWFCRDCLFHLPDALILRALRNFCDSKIKLLMMTNHINREGFANTDIK
                     AGGFRLVDFFSAPYNLPKDVLFRVTDYIEPYPPREMCVWTREQIAAALAETKAALQG"
     gene            10332..>10692
                     /locus_tag="VKU03_07975"
     CDS             10332..>10692
                     /locus_tag="VKU03_07975"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71238.1"
                     /translation="MTSPAVQPAQEFAGKTAIVTGGSGGIGRAIAAAFAWAGADVVIA
                     ARNNEAAERSAAAIRRETGARLAVIRTDVGVPAECAKLIDAAIAAFGAADILVNNAAL
                     FALVPLLDATSEDASRFF"
BASE COUNT         1745 a         3536 c         3396 g         2015 t
ORIGIN      
        1 ttgcgggaga gggctggggt gagggcgagc catccggcgc ttgccgatct tttggccgcc
       61 gcgcaccctc atccggcgct tcgcgccacc ttctcccgca agtgggagaa gggattgggc
      121 cgctgtgcgg cctacgtcga cacggcgttc gcgagcgcaa acgaacgccg catcgcgccc
      181 ctctcccgct tgcgggagag ggctggggtg agggcgagcg acgccgaggt tcagtcattt
      241 tcgccgccgc gcactctcat ccagcgtgtc gtcccgttct cccggaagcg ggagaaggat
      301 gtagccgcgg tcggcgaccg cggctacagc gcgccgccgg ccaatcttgg ttaaccaatg
      361 tcgcgtaggg tcggtcgaat gacacctgcc ttgggggcgc ttgatgccgt cgcgcgctga
      421 acgcgggctt ttcgccgatt ggttccgcac ggtcgatcgc tggctgctcg gctgtttcgg
      481 cgcgctgatg gtgatcggcc tggtcatgac cctcgccgcg agcccggctg tcgccgagcg
      541 gctcaacttg ccgaccttcc atttcgtcaa tcgccaggcg gcgctgcttc tgccctcagc
      601 gctgctgatg atcgccacct ctttcctatc gccgcgctat gtgcgccgcg cggctttgat
      661 cgtctttgtc gtcagcctgg ctttgatcat tctggccctg atgttcggcg tcgagatcaa
      721 gggctcgcgg cgctggattt tcggcgttca gccgtcggaa ttcctcaagc ccgccttcgt
      781 cgtcctcgcc gcctgggcgt tttccgaagg cgccaacggc cgcacgaaat tcgctggcgt
      841 cggcatcgcg ctgctgcttc tgccgatcgc gatcgggccg ctgatcatgc agccggattt
      901 cggccagacc atgctgatct cgctggtctg ggcggcgctc ttctttatgg ccggcctgca
      961 ttggttctgg gtggcgggcg tcggcggcgt cggcgcggtc ggcgcgatgc tcgcctttcg
     1021 cttctcgccg catgtccatg agcgcgtgct gcgcttcatc gatcccgatt ccagcggcgg
     1081 cctggtcgat acgttccagg tcgacaccgc cttgcagagc attctttccg gcggctggct
     1141 cggtcgcgga ccgggcgagg gcgtctataa gcgcatcctg cccgacgccc ataccgactt
     1201 cgttttcgcg gtgacgggcg aagaattcgg catgatcgcc tgtttggcgc tgatcgcctt
     1261 tttcggcttc atcgtgctgc gcggcctgct ccttgcggcg cgcaacgaag acccgttctg
     1321 ccgcttcgcc gccgccggcc tcgtcatcat gttcggcgtg caaagctgga tcaatatggc
     1381 ggtgaacctg cgcgccattc cggccaaggg gatgacgctg cccttcatct cctatggcgg
     1441 ctcgtcgatt ctcgcgctcg ccctcggcat gggctttctg atcgccctga cccgcaagcg
     1501 cccgcgcgcc gcgctgaccg cggcgtcctt ggcggaggcg ccttgagccg ggcgcgcgcg
     1561 attctcatcg ccgccggcgg aaccggcggc catctctttc ccgctcaggc cctggccgcc
     1621 gccttggcgg cgcgcggcgt cccggcggaa ctggcgaccg acgagcgcgc gctgaaatat
     1681 ggcggcgatt ttccggcgcg ggcgatccac gccattcctt ccgcgacgcc gaccggggcg
     1741 gggatcgccg ccaaggcgcg cgcgacgctg acgctcgctc gagggacctt tgtcgcccgt
     1801 gcgctgatca ggaaaatcgc gccgctggcg gtgatcgggt ttggcggcta tccgacggtg
     1861 ccgccgctcc tcgccgccgc ctggctcggc ctgccgactt tgctgcacga gcagaacgcg
     1921 gtgatgggcc gcgccaaccg cttcctggcc ggtcgcgtcg gcctcgtcgc caccggcttc
     1981 cctgcgctga agggggtgga cgacaagatc cgcgccaagg cgcgctatac cggcaatccg
     2041 gtgcggcccg ccgtcatcgc cgctgcggcg acgccgtttc ctgatttcgc cgacggccgc
     2101 ttacgcctgc tcgtcaccgg cggctcgcaa ggcgcgcgcg tcatggcgga tgtcgtgccg
     2161 gcggcgttgg ccctgctgag cgaaggcgag cgccggcgcc tgcgactgac ccagcaggcg
     2221 cgcggcgagg acaagcagcg cgtcgcgcag gcttgcgccg cgctcgattt cccggtcgag
     2281 ctcgccgaat tcttcagcga tttgccggac cgcatcgccg ccgcgcatct cgtcatcggg
     2341 cgagccggcg cctcgaccgt gtcggaactc gccgtgatcg gccgcccgtc gatcctggtg
     2401 ccgttcccgc atgctttgga tcaggaccag gcggccaacg ccgcgacgct cgaagcggcc
     2461 ggcgctgcgg aggtcgtgag gcagcgcgaa ttcacgcccg ggcgcctcgc cgcctcgctg
     2521 cgcaaggcgc tcgccgattc cgcgccgctc gccgccgccg cggcggcggc gaaagccgcc
     2581 ggcgttgccg acgcggcgga acggctcgcc gatctggtcg tcacggccgc cggaatcaaa
     2641 agctgacggc tcgcgcgatt tgagggcgcg tcgcggcgac gcttgcttcc cccgcgcttc
     2701 gccttatctc tgatccacaa atcgagtgcg agggctctta gcgcatggcc aactggaccc
     2761 tgcttccgga ccggagcgtg ctcagcgttt taggagaaga cgcggcgggc ttcctgcatg
     2821 ggctcgtcac cagcgatgtc gagagtctcg cagccggtca ggcgcgcttc gccgcgctgc
     2881 tgacgccgca aggcaagatt ctctttgatt ttttcattta tcgcgcgccg gaagagacgg
     2941 gcgcggcttt ctttatcgat tgtccgtcgc agaacgccgg cgatctcgcc aagcgccttg
     3001 gcttctatcg cctccgcgcc aaagtgacga tcgccgatct cagcgcggac aaggcggtcg
     3061 ctgtctgctg gacggatccg ccgcaagccg ccaacggcct cgttttcgcc gatccgcgcg
     3121 acgcccgcct cggctttcgc gccattctgc cgcgcgcggc ggcgccggcc ttggatcaag
     3181 cgcgtctcga ccaatacgaa gcgcatcgca tcgcgatcgg cgcgcccaag ggcggcgtcg
     3241 atttcgccta tggcgacgct tttccgcacg acgtcaatat ggacctgctc cacggcgtcg
     3301 atttcgacaa gggctgctat gtcggccagg aggtggtctc gcgcatgcgc catcgcggcg
     3361 gcgtgcgcaa gcgcattgtc agggtgaaac tggctcaatc cgcgccaggg gccgggacgc
     3421 cgatccttgc cggcgagtct ccgatcggcg cgctcggctc gaccgccggc ggcgaaggct
     3481 tggcgctttt gcggatcgac cggcttgccg aggccgagca ggccgggcgg ccgttgaccg
     3541 ccaatggcgt cgccgtcgtc gccgaatgcg cgcctgagtg actttagttt ctttaaatca
     3601 tggatattta tttagttgaa ctaaacttat tggacgttta gactgcgttt catgaagctc
     3661 agcgattggc tgacgcgcca caacgtcaaa cgcgcggatt tcgcccgccg catcggcgtc
     3721 tcgcccggcg cgatcaccca gatctgccgt gacgagggcg cctggatttc gcgcgagacc
     3781 gcggagctca ttttcaagga gactcgcggc gcggtcacgc ccaacgattt tctcggcgcc
     3841 gtcttcagcc ccaatcagga aagcgaaatg tcccattcgg tgaccgaagc ggtcgaagcc
     3901 ctggcggcgg gggagatcgt catcgtcacc gacgacgacg atcgcgaaaa cgagggcgac
     3961 ctcatctgcg ccgcgacgtt atgcaccacc gagaagatgg ctttcatcat ccgctatggc
     4021 tgcggcatcg tctgcgcgcc gctgacgacc caggaggcgc agcgcctgca cctctcgccg
     4081 atggtggcga tgaacgaggc gccgctcggc acggccttca ccgtctcggt cgacgtgaag
     4141 cacggcctca ccaccggcat ttccgccgaa cagcgcgcca acaccgcccg cgccctggcc
     4201 aatcgcaata tggcggcgag cgatttcgcg cggcccggcc atgtttttcc gctcatcgcc
     4261 aaggagggcg gcgtattgat gcgctccggc cataccgagg cggcggtcga tctctgcaag
     4321 ctcgccggct tggcgccggt cgcggtgatc tgtgagctcg ccaatgacga cggcacagtc
     4381 atggtcgggc cgcaaatcga agccttcgct caaaagcaca atctgaagcg catctcggtc
     4441 gccgatctca tcgcctatcg ccaggcgcgc gagaagctgg tcgagcgggt ggcgagtttc
     4501 ccggtcaaga gcgagatcgg cgagctcgcc ggctacgcct accgcacgcc gttcgactcg
     4561 gttctgaatt tcgccttcgt ctatgggccg atcggcgacg gacgcgacgt gccggcgcgc
     4621 ctgcacagga cggatattgt cagcgatatt ttcggcggcg gcgtcattcc gcgcacgctc
     4681 aagcgcttca aacaggaagg acgaggggtt ctggtctatt tgcgcgacgg ctcggccggc
     4741 gttccgacca atctgacgac gaccgatccg gcggcgagct cggaaaacgt tcgcgcgacg
     4801 caatggcggg aaatcggtct cggcgcgcaa atcctgcgcg atctcggcgt gtcctcgatc
     4861 cggctcagga ccgagaatcc gagaaagtac gtcgggcttt ccggcttcgg catcgagatt
     4921 tccgccgtcg aagggctcga ctgctgaagc ctgttggcta cccggacgct aagcgcttcg
     4981 acgagtccag acgacgaatg tcaaggcgtt ggcctcgtcc aaggtctgaa ccggccccgg
     5041 ctccaatcca tattccttga gaacgtcttc cggcgtccgg ccggggccga cgccgggaaa
     5101 gaccgtcacg ccgcccggca aggcgacgcg ggcggcgcgg caaaggtaga attcgtcgaa
     5161 gccgaggtcg aggaacagct tatagacgta aggcccgccg agaatggcga tcgtcccttt
     5221 ttcgagaccg agcttcaagc aggcttcggc aaaggatgcg cccgccgggt tccagaggaa
     5281 gccgcgcgga aagttcggat cggcggccag cgaggcgatc tgccgggtca ggaccaggcg
     5341 ccggcgattg ggcgaatcgg cctggtattc ataggagttg cggccctgga cgacggcgtc
     5401 ggcccgcgcc agttcgcgct cgaagaaatc cttatcggcg ccgaaaatca gcgcgttcgg
     5461 cattaatccg gtcgaatcgg cgatcatccc gtccgacgag acgatggcgt agccaagaat
     5521 gcgaaaaggt cccggcatgg gagaggtctc gtgacgcggc ggttaggcgg cggcgagcgg
     5581 cgggttgccg gacgcgggcg aagggccgat tgcgagaacg accgatgaca tgagcgcccc
     5641 cgttgtctct tggcgtcccg ccgacctcgt caagctgtag ccgcggcgcc cttccggatc
     5701 aagccgcgcc ctgaggcgcc ggcattggat ttgaccggca aaagccctat ctctggccga
     5761 gatcgtcgct cgccccggcg aagcgagatc gatttctcgg agctttcagc cgcatgtcga
     5821 ccgattacgt ctcggaaaat ctctcgcttc ccgacctttc gcgccatttc cccaatatga
     5881 tcgccggcga taagaagagc gcgacctggc cctatttcag gaaggagatt gatcatacct
     5941 ggtacgtcga tcggcgcgat ccgcagatcg gcttcatcaa caaggacgag gccgccatcc
     6001 tctacgccaa tgcgcggctt ttcgccggca agaacggctt ggagatcggc gcctggcgcg
     6061 gctggtcgag ctgccatctt ctcgccgccg gcctcgccac attgcatatt gtcgaaccga
     6121 agctggccga cccggattgg aatcgcgaat tcgccgcagc ggtcacggcc gtcggcggcg
     6181 gcgagcgggc gattttcgtt cccggcctca gccccgccga agtcgtccgc ctcggcgaaa
     6241 gcggccagcg atggtccttc gccttcattg acggcgatca tgatggcgat gctcccaaga
     6301 acgacgcttt ggcgaccatc cgcttcctgg agccgaccgc gatggtcctc tttcacgatc
     6361 tcgcttcgcc ccatgtggcg gcggcgctgc atgcgctgaa ggcgcaaggc tggcgcaccc
     6421 tgatctatca aaccgcccag atcatgggcg ccgcttggcg cggcgaggtc gcgcccgccg
     6481 cgcacaagcc cgatccgcgt cagacctggc gtctgccgga tcatctcgcc ggcctggcga
     6541 cgtgacggcc gccatgactt ggcgtcggct caaagctcgg cgctgacgat ggcccaccag
     6601 aagccgctat aggaaagctc gaagccgatc actttcagcc tgagatcgat gtcgtcgagc
     6661 gcctcataag ccgaattgct tgaaatcgag acgatcgcca tcccgctctt ctttaagaac
     6721 cggcccgcct cccgcaaata ggcccgggtg atctggccgc cgggatcgca caagctgtag
     6781 cgttcgacgc tgctctccgg ctccttgtcg atcagcggcg cattgaagat gatcaggtcg
     6841 aaacctcgct cctcgatctt ctcgaacaaa tcggactgga cggtcttcag cgccacgtcg
     6901 ttcgcgcggg cgttgcgccg aatggactcg acagcaaccg gcgatatgtc cgaagcgacg
     6961 acctcggcgc cccaatgctt ggcgagatag agcgaaatcg cgccgcagcc ggcgccgatt
     7021 tcgaggattt tgcggatctt gttcttgtcc atcgccttca agttgcgcaa aaaaagctgc
     7081 gagcttgaat tcggcgtcgg atggtagacg ccggccggcg cgtcgatcga aagaccgtcg
     7141 atcaggaaat gcgccggcac gttctgctcg accacatggg cgtgatggga cgcggaaatg
     7201 tcgaccgcgg tgttcggcac gagcggcgag aggccgagct tgccgagcac ggaggtgcgc
     7261 gcaatctctt ttctgcgaac ttccaacatg gctctcgcct ttgaaaactg aaggaaattt
     7321 cgatctcccc ccaggagatc gacaagcggc gcgcgacctt tgcgcaattc cgttacgttg
     7381 gcaaccgagc ccgagtttca aggccgatgt cgcttgtaga gcctgtccgc catcgcttct
     7441 gtggcgaacg ccacatctcg gccgccgcgc gccgcgcgaa acatcaaggg ttctcaattt
     7501 gttgcatgat cgccggcggc ccaggtaaaa ggctgggcaa gggccggcgg gcggcgttcg
     7561 cgtcgcccgg cagtcaagtc gtcgaggagg cgcgtatggc gaagtataaa atcctcagcg
     7621 aggccgatgt cgcgacggaa gtggcgaaac tgaaggattg gtcggtcaaa agtccgaaca
     7681 tcgaagcgtc attcgacttc aagacgtttc gcgacgccat ttccttcatc gtccaggtgg
     7741 cgatcgaggc cgaagtcatg aaccatcacc ccgagttcca caattcctac aacaaggtct
     7801 cgttctcctt ctgcacgcat gacgccgggt tcaagatcac cgatcttgat atcgagacgg
     7861 cgtcgcggat cagcgcgctg gcgcgccgct tccagagcat ggcctgaaca tcgtcgatgc
     7921 tggacgtcca aaaaaaggat aaggcgggcg cgacgcccgc cgcggaagcc gagggcaagc
     7981 cgcctctgct gccggttggc gcctggcaaa ccgcgggccg caatctgctg cccggaaaac
     8041 cgatctcgcc cgccgacatg ccggactaca cggtcaagcg ggacgatctc gccgaaatct
     8101 atgccgccgc cttcctgcgg cgactgaagg cgctgaacgc tttcgtcaag caggccggcg
     8161 agcggctgga gggcgggata ttctattggc atggcgacgc cgcctatcac gacagtccgc
     8221 ccgcggcgga tctcgcgccc gcccgccgca atctctggcg cgcggcgcgc ttcaagcgtt
     8281 cgatgctcga aatcggcgtc aatgccggcc atagcgcgct gctggcgctc tccgccaatc
     8341 cgcgcctcgt ctataccggg atcgatctcg gcgttcatcg ctatacggag caatgcgtcg
     8401 cctatctggc ggcggagttc cccggcaggg tgcgctacct gcagggcgat tcgcgcgacg
     8461 tgctgccgca actcgccgcc cgtcgcgacg catgggattt cgatctctac catgtcgatg
     8521 gcggccatac cgacgacgta tgtcggaccg atgtcggcaa ttgcctgacc atcgccgaag
     8581 gcggccgcgg ccggcatctt ctgctcgacg atatcaacgc cagttggatt ttcgacgtct
     8641 attgcgagtt cgtcgccgcg gggcggcttg cgaccgaaac gttcttcggc gattgggagg
     8701 acgtcaatcg caatgtcctg gcgagaattc tctgaccggg cctcgacgtg acgtttcttt
     8761 ttcttctctt gcgcggaatt aaggcgtgag cgactcgcaa tttgcgacct tctggcgcgg
     8821 gccgctcaac ccgatcgcct atagctgcct cgcctccttt ccttcggtcg gggcgagcct
     8881 ctcggtctac gcttatgagg gcgatctcga actcccgccc ggcgtggagc gagcggacgc
     8941 gcgacggatt tgccccgacc cgtctctgct ccaccgctat cgcgtcaacg gcaagccgtc
     9001 gatcgcgatg ttcgccgata tgttccgcta taagctcgtc cgcgatacgg gctgctgctg
     9061 ggtcgacgcc gatcttgttt gcttgcgccg ccccgatttt tccggcgagg cgatcgtcta
     9121 tggccgccag gccgaggcgc gcggcaaggc gctgatcaac aacgcggtgc tgaagctccc
     9181 gcccgaccat cctctgctga ccgatctgat ccaaagggcg gaagccgcgg tcgatatcga
     9241 tcaaagctgg ggcgcgatcg ggccgtttct cctgaccgac ctggccgaga agcatggcgt
     9301 cgatcatctg gcgcgcgatg aaaccgaatt ctatccgatc gacgccgacc gtttctggct
     9361 gccgcttttg ccggcaacgt ccgaggccgc ggccaaggcg acgagcgggg cgacctttct
     9421 ccatctctgg agcgccatgt tcgagcggtc gcattacgac ctatcaagct gtccgccgcc
     9481 gggctctttc cttcacgacg cgttcaagcg cttgggaacg ctgcatcgct tcccgcgagt
     9541 ctatgacgag cctatgcttt cacgcttgct gtcggaatgg atcgccaaag gcgcgccgag
     9601 cagcgccgcg cttgcctctt cctgaggcgc gtgggaggaa ggacgaaggc caaatcctta
     9661 aaactcggtc ggctggggac catgtcggag ctgaagagaa tcttcgagcg catcgcggcc
     9721 cggaacaaat ggcgcaatcg cgagagccgc agcggcgacg gctcgacttt gttctacacc
     9781 cgccatttgc gcagccgact cgaaactttc gtgcgcgagt tcgacatccg gaccttcttc
     9841 gacgcgccgt gcggcgattt caactggatg cgggcggtcg cctttccgcc gcgcgtcacc
     9901 tatatcggcg gcgacatcgc gctcgccttg atcaaggaga atcgcaagcg atattccgag
     9961 ccgcgccgcc ggttcctcca attcgatatt gtcagcgatc cgttcccggc cgccgacgtc
    10021 tggttttgcc gcgactgcct ctttcacttg cctgacgcgc tgatccttcg cgcgttgcgc
    10081 aatttctgcg attcgaagat caagctgctg atgatgacga accacatcaa tcgcgaagga
    10141 ttcgccaata ccgacatcaa agccggaggt ttccgcctgg tcgatttctt cagcgcgccg
    10201 tacaatctgc cgaaggacgt gcttttccgc gtcacggatt acatagagcc ttatccgccg
    10261 cgcgagatgt gcgtctggac gcgtgagcag atcgccgccg ctttggctga aaccaaagcg
    10321 gcgctccagg gatgacgagc ccggccgtgc agccagcgca ggaatttgcc ggcaaaaccg
    10381 ccatcgtgac gggcggcagc ggcggaatcg gccgggccat cgccgccgcc ttcgcctggg
    10441 ccggcgcgga tgtcgtcatc gccgcccgca acaacgaggc cgcggagcga agcgcggccg
    10501 ccattcggcg cgagaccggc gcgcgtctcg cggtcatccg gaccgatgtc ggcgttccgg
    10561 ccgaatgcgc caagctcatc gacgccgcaa tcgcagcgtt cggcgcggcg gacattttgg
    10621 tcaacaacgc cgccctattc gccctggtcc cgctgcttga cgcgacgagc gaggacgcgt
    10681 cgcgcttttt ca
//
LOCUS       DATGJW010000256         5480 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_48865, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000256 DATGJW010000000
VERSION     DATGJW010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5480)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5480)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5480
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48865"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1509
                     /locus_tag="VKU03_07980"
     CDS             <1..1509
                     /locus_tag="VKU03_07980"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ71239.1"
                     /translation="NASPLAVSLVQVAVSLPLFLFTLPAGALADVVNARRLLLVVETG
                     VILVSAVFAVLVSLGLSGVGALLATTFLLGAAGALTSPAWAAIVPQLVPRQDLGSATA
                     ANSAGFSLARAIGPALGGALIAALGVASAFWVFAVSNFGVVAALLWWRPPHKSGESLP
                     AERFLSAVRNGVRFAANSRYLDASLARALAYFPFACAYLALLPLIARMQTGGGPENYG
                     LLLGANGLGAIGGSFALNPLKARLGADRLVAAGTLATAVALALFGVARDLAVATAACL
                     IVGAAWTLVLSVLYVSAQVSLPDWVRGRGLAIFLTVIFGATTIGSVVWGQVAAMKGLP
                     FAHFAAAVGVVLAAPLTWRWKLQAAEGLDLTPSMHWRAPSGLRKVENNQGPVLVSLEF
                     RVEAENRAAFLLAAEELKHERKRDGAFAWSLFEDTVESGRFVEHFHMESWLELMHSRE
                     RVTNADRVLEERVIAFAKGPPVVSLLVASERGRRFKPGRQRNARTTGPAQER"
     gene            1524..2165
                     /locus_tag="VKU03_07985"
     CDS             1524..2165
                     /locus_tag="VKU03_07985"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLJ71240.1"
                     /translation="MKIGVLGSGPVATTLGGGFLKHGYDVALGTREPAKLAEWRAKNP
                     GAKIVSFAEAAAFGEIVVLAVGGAVALDALALAGAANLKGKTVIDACNPIGGGPPVKG
                     VLTFFTSLTDSLMERLQKAYPDARFVKAFNSVGAAQMVNPQFAGGKPTMFICGDDSAA
                     KKQVEAILDQFGWETADMGAVEAARAIEPLCMLWCIPGLARNDWSPHAFKLLR"
     gene            complement(2885..3160)
                     /locus_tag="VKU03_07990"
     CDS             complement(2885..3160)
                     /locus_tag="VKU03_07990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71241.1"
                     /translation="MARFSLRLSALAAVAGAGAFLFATASEAAMPMAPLTTGADIVHV
                     AQGCGPGRWRGPGGWCRGPGWRGPGWRGPGWRHCWRGPYGHLHCRWR"
     gene            complement(3324..3665)
                     /locus_tag="VKU03_07995"
     CDS             complement(3324..3665)
                     /locus_tag="VKU03_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71242.1"
                     /translation="MFRAADILGRAAFRFVREQVVIGREAVDHLAQARVGAIVRQGAH
                     ICCAAPMMLAARASEPEDNSRLLEMARAWEALGKESIEGGPKSYNLSFWRGRREAEAA
                     RKIGLSRRTFF"
     gene            complement(3704..3922)
                     /locus_tag="VKU03_08000"
     CDS             complement(3704..3922)
                     /locus_tag="VKU03_08000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71243.1"
                     /translation="MSWVRVQNYRCYAADCMELAGKASETDRRRLVEMAAGWHELATM
                     LEAYMEAHGGREPPYQPVFGPGEAKPSH"
     gene            4370..4798
                     /locus_tag="VKU03_08005"
     CDS             4370..4798
                     /locus_tag="VKU03_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71244.1"
                     /translation="MLIEEAKAGGATSAEKQSFWTAIDAFNNMWTIYNPTGVMQPDSK
                     LYQVLHPDVIIFNVTDDGQRASGLRDAVDKLYALAGANFDPRHNPGPPYKYGPPNFTQ
                     AHKVSGIALWTDCDHAGSETIPYTFHFQNNLIVRMRAKIE"
     gene            5016..>5480
                     /locus_tag="VKU03_08010"
     CDS             5016..>5480
                     /locus_tag="VKU03_08010"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLJ71245.1"
                     /translation="MLREAAEIWDACRLLSGEILDVGCGLGGGAIFWAQEFGARVTAV
                     TCVPSHAELVARFVQAAGVASLVKPLVCDAPFVPGENRFDAVVAVDSSGYLPRDEWFA
                     RVAALLRPGGSLFVIDCFLENPKYAELFNSHWRTQIGTTDEYFTAARKSRLES"
BASE COUNT          942 a         1807 c         1713 g         1018 t
ORIGIN      
        1 aacgccagcc cgctcgccgt gtcgctggtg caggtcgcgg tctcgctgcc gctgttcctg
       61 ttcacgctgc cggccggcgc gctcgccgac gtcgtcaatg cgcggcggct gctgctcgtc
      121 gtcgagaccg gcgtcatcct cgtctccgcg gttttcgccg tgctcgtctc gctgggcctg
      181 agcggcgtcg gcgccttgct cgccacgacg ttcctgctcg gcgccgccgg cgcgctgacc
      241 tcgccggcct gggcggcgat cgtgccgcag ctcgtgccgc ggcaggatct cggcagcgcc
      301 accgccgcca acagcgccgg attcagcctg gcgcgcgcga tcggcccggc gctcggcggc
      361 gccttgatcg ccgcgctcgg cgtcgcctcc gcgttctggg tcttcgcggt cagcaatttc
      421 ggcgtcgtcg cggcgctgct atggtggcgc ccgccgcata agagcggcga gagcctgccc
      481 gccgagcggt tcttgagcgc ggtccgcaac ggcgtgcgct tcgccgccaa cagccgctat
      541 ctcgatgcga gtctggcgcg cgcgctcgcc tattttcctt tcgcctgcgc ctatctcgcg
      601 ctcctgccgc tgatcgcgcg catgcagacg ggcggcgggc cggaaaatta cggcctgttg
      661 ttgggcgcca acggcctcgg cgcgatcggc ggctccttcg ctttgaatcc gctcaaagcg
      721 cggctcggcg ccgaccgtct cgtcgcggcc gggaccctgg cgaccgccgt cgcgctggcg
      781 cttttcggcg tcgcgcgcga tctggcggtc gcgaccgccg cttgcctcat cgtcggggcg
      841 gcgtggacgc tggttctctc ggtcctctac gtctccgctc aggtctcgct gcccgactgg
      901 gtgcgcgggc gcggattggc gatttttctc accgtgattt tcggcgcgac gacgatcggc
      961 agcgtggtct ggggccaggt cgcggcgatg aagggcctgc ccttcgccca tttcgccgcg
     1021 gcggtcggcg tcgtcctcgc cgcgccgctg acatggcgct ggaaattgca agcggccgaa
     1081 ggcctcgatc tgacgccgtc catgcattgg cgcgcgccgt cgggcctgcg caaggtcgag
     1141 aacaatcagg ggccggttct ggtgtcgctc gaatttcgcg tcgaagcgga aaatcgcgcc
     1201 gcctttctcc tcgccgccga ggaattgaag cacgagcgca agcgcgacgg cgccttcgcc
     1261 tggagtctct tcgaggatac ggtcgaaagc ggccgcttcg ttgagcattt ccacatggag
     1321 tcttggctcg aactgatgca tagcagggag cgggtcacca atgccgaccg cgtgctcgaa
     1381 gagcgggtga tcgctttcgc caaggggccg ccggtcgtct cgctgctcgt cgcctccgag
     1441 cgcggccgcc ggttcaagcc gggacgtcag cgaaacgcgc gaacgaccgg gccggcgcag
     1501 gagcgctaag ggagtttttg cttatgaaaa tcggcgttct cggttcaggt cccgtcgcca
     1561 cgactttggg cggcggattt ttgaagcacg gatatgacgt cgcgctcggc acgcgcgagc
     1621 cggccaagct cgccgaatgg cgcgcgaaaa atcccggcgc caaaatcgtc agcttcgccg
     1681 aagccgcggc gttcggcgag atcgtcgttt tggcggtcgg cggcgcggtc gccctcgacg
     1741 ctttggccct tgccggcgcc gccaatctca agggcaagac cgtgatcgac gcctgcaatc
     1801 cgatcggcgg cgggccgccg gtcaagggcg tgctgacctt cttcaccagc ctgaccgatt
     1861 cgctgatgga gcggctgcaa aaggcttatc ccgacgcgcg tttcgtcaag gcgttcaact
     1921 ccgtcggcgc cgcgcagatg gtcaatccgc aatttgccgg cggcaagccg accatgttca
     1981 tttgcggcga tgattccgcc gccaagaagc aggtcgaagc cattctcgat cagttcggct
     2041 gggagacggc ggatatgggc gcggtcgagg cggcccgcgc gatcgagccg ctctgcatgc
     2101 tctggtgcat tcccggcctt gcccgcaacg attggtcgcc gcatgctttc aagctgttgc
     2161 ggtgagggtt gcgccgtcgc gctcggtacg ctcgcctaat gcggcggcgg aagcccgtcg
     2221 cacgcggcgt caaatccgcc gcccgctttc tcgctcaact tggcgagcct cgtccaaagg
     2281 aaggccagtt cgagcagaac ggcgcagctt tccgcgcttc tcacgatgag ggcgtcttcg
     2341 atgcaggcgc gcgcctgcct gcggtaggcg gccgcccgac ccctggggcg ctgatcgtcg
     2401 gtcattcgag caggcaagcg aagcctttgc gactcaaaag cccaacaagc atcccgtcgc
     2461 gatccgccca aaatgagagc gtggagcgga ttctctgtgg aaaaatcgcg gcgcctgatc
     2521 ggtccgaagc agcttaagta agcgaaaggg cgggacgcgc ctgggcgtcc cgccctcgtt
     2581 gcgctggccc tgcgccagcg ctcatcacaa agcctcgctc acgtaccaag cgcgtaacct
     2641 cgcgcgcgcc gcctcgttcc gcgcgcgaag cacacgtttt cgtgagcgcg aaatgcgcgc
     2701 ccgcatctag ccgctcgcgc tttcttcctc gcgccaagcg atcgcgtcgg cggctacgcc
     2761 gaacaaacac gttttcgtga aatcgcgcga tcgcgtcgcc gccggactcg gggagcttcc
     2821 cggccgagat gcgcgacaag aacttgagac cccgccgctt tcgcggcgag gtcgaaccgg
     2881 cgacttagcg ccagcggcag tgcagatggc cgtagggacc gcgccagcaa tggcgccagc
     2941 ccggcccgcg ccagcctggc ccgcgccagc ccggcccgcg gcaccagccg cccggcccgc
     3001 gccagcggcc agggccgcag ccttgcgcga catgcacgat atcggcgccg gtggttaacg
     3061 gcgccatcgg catcgccgcc tcggatgcgg tcgcgaacag aaacgcgccc gcgcccgcaa
     3121 cggcggcaag cgccgatagg cgaagcgaaa atctagccat cgagttgcac tcctctagct
     3181 ccccccaatc agccgatctt ggcgccggag acatgaactc ggcgtgaatg gatggagcgc
     3241 acgacgccgc gaggcggcag acggcttttg gcgcgatgaa tgcgccgtcg agctgaggcg
     3301 cgccgcgcga gcccggtcgc ggctcaaaaa aaagtacgtc gcgagaggcc gattttccgc
     3361 gcggcctccg cctcccggcg tccgcgccaa aagctcaaat tgtaagattt cggcccaccc
     3421 tcgatcgatt ccttgccaag cgcctcccag gctctcgcca tttccagcag ccggctattg
     3481 tcctccggct cggacgcgcg cgccgcgagc atcatcggcg ccgcgcagca aatatgcgcg
     3541 ccttggcgca cgattgcgcc gacccgcgct tgcgcgagat gatcgaccgc ctcgcggccg
     3601 attacgacct gctcgcgaac aaatcgaaaa gcggcacggc cgagaatgtc cgccgcccga
     3661 aacacgatag aggttctcag gatccgggcc cggacccgcg ctgtcaatgc gagggcttcg
     3721 cctcgcccgg cccgaaaacc ggctgataag gcggctcccg gccgccatgc gcttccatat
     3781 aggcttcgag catcgtcgcc agttcgtgcc acccggccgc catttcgacc aggcgccgcc
     3841 gatcggtttc gctcgccttt ccagcgagtt ccatgcaatc ggcggcatag cagcggtaat
     3901 tttggacccg aacccagctc atgcgccgca caacgcggcg cgcgcgacgt tggttccggc
     3961 tagcaggcta gattctcgcg agaggagcct tcctgagtcc gcaaatttca gttgtatata
     4021 ccgataacaa acgcaacagt tgccacctat tgatcctgcg acaaatgggg cacctatagg
     4081 actagcgcac tcttttgaac atcggaagct tggtcggtgt cgctgtttat gtttaaacgc
     4141 gattatcctg cgacctcgca acgacccttg accaaattac gagtgtgcga caaaatcaaa
     4201 tggctttgtt tcagttcatg ccagctccgc ggaagcgtta agctgacgag taatagtgct
     4261 gccctaagcg ggagctcaca tggctgaaaa tataacacgc ccagatcgaa acggaaagcc
     4321 gactcggcga agcgtactcc tgacaatggt aagtgcaggc gcgtctggaa tgctgatcga
     4381 agaggcgaag gcgggagggg cgacttccgc ggaaaagcaa agtttttgga ccgccatcga
     4441 cgcatttaac aacatgtgga caatttacaa ccccacagga gtaatgcagc ccgacagtaa
     4501 attgtaccaa gttctgcacc ctgacgtgat catttttaac gttaccgacg atgggcagag
     4561 ggcttctggc ctgcgggacg cagtcgacaa gctctatgcc ctagcgggcg caaatttcga
     4621 ccctcgtcat aatccgggac caccgtataa atacggacct ccgaacttta ctcaggccca
     4681 caaggtttcg ggaattgcac tttggaccga ctgcgaccac gccggttcag aaacaatccc
     4741 gtacactttt cactttcaga acaatctcat cgtaagaatg agagctaaaa ttgaatgaac
     4801 tacgttgacc tctcctccgg cggataaaag aattgacctc agcgccggct ttacttgaca
     4861 tcattactta tttcgatcgc aagacgccgt ccattttaga gcgctatggt ccggggccac
     4921 gtgtccacta tcacacgggc ctcgtggacg atccgccgcc gaaacaagcg tcagtcctgc
     4981 aattgcggcg cgcgcttgtc gaagggcagg aactcatgct tcgagaggcc gccgaaattt
     5041 gggacgcctg cagacttctt agcggcgaaa ttctcgatgt cggttgcggg ctcggcggcg
     5101 gggcgatctt ctgggcgcag gaattcggcg ctcgcgtgac ggctgtgact tgcgtgccct
     5161 cgcacgcgga gttggtcgcg cgtttcgtgc aggcggcggg agtcgcctcg ctggtgaagc
     5221 cgctcgtctg cgacgcgcct ttcgtgcccg gcgagaatag gttcgacgcc gtggtcgcgg
     5281 tcgatagctc cgggtatctt cctcgcgacg aatggttcgc ccgggtcgcc gctctccttc
     5341 gtccaggcgg ttcgctcttc gtcatcgact gtttccttga aaatcccaaa tacgcggagc
     5401 ttttcaacag ccattggcgg acccagatcg gcacgaccga cgaatatttc acagcggctc
     5461 ggaaaagcag gctggagtcg
//
LOCUS       DATGJW010000257         5904 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_153180, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000257 DATGJW010000000
VERSION     DATGJW010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5904)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5904)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5904
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153180"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..555)
                     /locus_tag="VKU03_08015"
     CDS             complement(<1..555)
                     /locus_tag="VKU03_08015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167266.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HLJ71246.1"
                     /translation="MAVHFSPAEMAARKQRLLAAIAAQKLDGLLLFAQESMYWLTGYD
                     TFGFCFFQCLYVGADGRTALLTRSADLRQAQRTSNIEDIRIWKDGGDADPARDLRRML
                     ENLGAKGRLGVEYDSYGLTHFNGRRLEAALAGWAELADASRLVPALRVTKSAAELAFV
                     REAARLSDLADRAAIEQIAPGADEG"
     gene            821..2623
                     /locus_tag="VKU03_08020"
     CDS             821..2623
                     /locus_tag="VKU03_08020"
                     /inference="COORDINATES: protein motif:HMM:NF012861.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chloride channel protein"
                     /protein_id="HLJ71247.1"
                     /translation="MNKPIEQPPRTEPPSASASRIDRALPGWMRAFVRTRESGLLAAA
                     VIIGIASGFLVSALNIAAQRMHEMLFAIPRGASLSVTRSPDLWRTVGAPVLGGVALLG
                     LALWAKDRFRGRLADAVEANALRGGRLSAGGSAYIVAQTLVSNGFGASVGLEAAYTQL
                     SSAFSSLFGRALGARRADMRLLVGCGAAGAIAGAFDAPLAGAFYAFEAILGAYTLSSL
                     APVLISAILASMVTRTVLARPEALIHAPLPAPLIELGVHIIALAVIAAAASIALMAGV
                     ALLERVWSLTGLPGKWRPIFGGVLVGGLGLVAPEALGAGHGALGVNLSTTPLISQVAL
                     ALAIKWLSSAISLSSGFRGGLFFCSLLIGALAGRLYGEGLDAIWPAAMIDPAAASVLG
                     MAAVGAGVIGSPVAMTFFALETTNDFRVTVAALMSSALTGLIVRETFGYSFATWRFHL
                     RGEAIRGPQDVGWMRDIKAARLMRKDFRAVAADRTIAAARVLFPVGSAREILLTDENE
                     AYVGIVLVSDLHAAAHNDSEPITKLAQWRNNFITAQTTIRAAVDAFDEAEADTLAVVA
                     DAQSRKIVGVLSEAHALRVYGRELEKQNQAFLGR"
     gene            2739..4769
                     /gene="uvrC"
                     /locus_tag="VKU03_08025"
     CDS             2739..4769
                     /gene="uvrC"
                     /locus_tag="VKU03_08025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0009381 - excinuclease ABC activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrC"
                     /protein_id="HLJ71248.1"
                     /translation="MPAPKIPPAEPLLDAPPGPTLDEDESEAGFELALAEDEAAPNAL
                     ARGAAAIKAHWRQAPLGPGVYRMIAADGEVLYVGKARSIKKRIASYIRPEMQSARIAK
                     MIARTASMVFVSTETEAEALLLEANLIKQLKPRYNVLLRDDKSFPYILVTSGHRAPQL
                     AKHRGARSIKGAYFGPFASAGAVGRTLNALERAFLLRTCSDSYFENRTRPCLLYQIKR
                     CAGPCTHEISDDDYAVLVQEAKDFLSGKSRAVRDRMAEEMRQAAEDLAFEKAARLRDR
                     IAALSAIQGEQAINPRSIAEADVFAVAEEAGQFCIEVFFFRTYQNWGNRAYFPRADRS
                     MSAAEVLDSFLGQFYADRPAPRLVLLSHDIESAPILSEALCVKAGRRVAVGRPQRGEK
                     RELVENALRNAREALSRKLTEAATQEKLLGALGAAFGFDEPIRRVDVFDNSHIMGTNA
                     IGAMVVAGPDGFMKAHYRTFNIKSAELTPGDDYAMMREVLTRRFARLAKAARRTELTE
                     SGETVPVAKPEPEAGAFPEWPDLVLIDGGRGQLEAAREVFAMLRIEGPRLVAIAKGPD
                     RNAGRETFFIPGREPFRLPSRDPALYFVQRLRDEAHRFAIGTHRAKRKREFVKSPLDE
                     IPGIGPARKRALLNAFGTAKAVARAGLADLEKVAGVNQATARIVYEFFHEGG"
     gene            complement(4808..>5904)
                     /locus_tag="VKU03_08030"
     CDS             complement(4808..>5904)
                     /locus_tag="VKU03_08030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translational GTPase TypA"
                     /protein_id="HLJ71249.1"
                     /translation="GNVIEQGRISKILAFRGIERSPIEEAEAGDIVAIAGLEKFNVAD
                     TLAALDVSEPLQAQPIDPPTLSMTFLVNDSPLAGTEGDKVTSRVIRDRLFKEAEGNVA
                     LRVEPAPSTEAFVVSGRGELQLAILIETMRREGFELAVSRPKVVLSRGENGQLLEPIE
                     EVVIDVDEEHSGIVVRKMSERKGEMVEMRPSGGGRLRLVFHAPTRGLIGYLGELLTDT
                     RGTAVMNRLFHAYAPWKGEIAGRRNGVLISNDAGEAVAYAMWNLEDRGPMMIEPGWKV
                     YQGMIVGEHTRDNDLEVNVLKGKKLTNIRTHSKDEAVRLTPPIQMTLEKALAYIEDDE
                     LVEVTPKSIRLRKALLDPNERKKAERARAS"
BASE COUNT          916 a         2023 c         1939 g         1026 t
ORIGIN      
        1 gccttcgtcg gcgccgggcg caatttgctc gatcgcggcg cggtcggcga gatcggagag
       61 acgcgccgcc tcgcggacaa aggcgagctc ggcggcggat ttcgtgacgc gcagcgccgg
      121 gacgaggcgc gacgcgtcgg caagctcggc ccagccggcc agcgcggctt cgagccggcg
      181 gccattgaaa tgggtgaggc catagctgtc atattcgacg ccgagccggc ccttcgcgcc
      241 gagattttcc agcatccggc gaaggtcgcg ggcgggatcg gcgtcgccgc cgtccttcca
      301 gatgcggatg tcctcgatgt tcgaggtgcg ttgcgcctgg cgcagatccg ccgaccgcgt
      361 cagcagcgcg gtgcgcccat ccgcgccgac atagaggcat tggaagaagc agaagccgaa
      421 cgtgtcgtag ccggtcagcc aatacatgct ctcctgcgcg aagagcagca gcccgtcgag
      481 tttctgcgct gcgatcgccg ccagaaggcg ctgttttcgc gccgccattt cggctggaga
      541 aaaatggacg gccatcgctg ctccttgcta ttcatggaaa atcgatttgc gccccggaca
      601 cgggcgaagc taagcagaat tttatgtctg cgcctaacgc atgcgggtca ccctcacgcc
      661 aaccctctcc cgcaagcggg agaggggggg cgccgtgcgg tttattagcg tttgggaatt
      721 tctcccggcg cttgggaatc ccttctcccg cttgcgcgag aagcgggcgg cgaagccgcc
      781 gcatgagggt ttgctgcggc ggctttgggc gcgcggggcg atgaacaaac ccatcgaaca
      841 gccgccacgc acggagccgc cgagcgcttc ggcgagccgc atcgaccgcg cccttcccgg
      901 atggatgcgc gccttcgtgc gcacccgcga gagcggcctg ctcgcggcgg cggtgatcat
      961 cggaatcgcc agcggcttcc tggtttcggc gctgaacatc gcggcgcaga ggatgcacga
     1021 aatgctcttc gccatcccgc gcggggcttc gctgagcgtg acgcgctcgc ccgacctttg
     1081 gcggacggtc ggcgcgccgg tcctgggcgg cgtcgccctg cttggcctgg cgctttgggc
     1141 gaaggaccgc tttcggggcc gccttgccga cgcggtcgag gccaacgcct tgcgcggcgg
     1201 ccggctttcg gccggcggct cggcctatat cgtcgcccaa acccttgtct cgaacggctt
     1261 tggcgcgtcg gtcgggctcg aagccgccta cacccagctc tccagcgcgt tcagctcgct
     1321 gttcggccgc gccctcggcg cgcggcgcgc cgatatgcgc ctgctggtcg gctgcggcgc
     1381 ggccggcgcg atcgccggcg ccttcgacgc gccgctggcc ggcgctttct acgccttcga
     1441 ggcgattctc ggcgcctata cgctctcctc gctggccccg gtcctgatca gcgcgattct
     1501 cgccagcatg gtgacgcgga cggtgctggc gcggcccgaa gcgttgattc acgcgccgct
     1561 gccggcgccg ctgatcgagc ttggcgtcca catcatcgcc ctcgccgtga tcgccgcggc
     1621 ggcgagcatc gcgctcatgg ccggcgtcgc gctgctcgaa cgcgtctgga gcctgactgg
     1681 cctgcccggc aaatggcggc cgattttcgg cggcgttctg gtcggcgggc ttggtctcgt
     1741 cgcgccggaa gcgcttggcg cgggccatgg cgcgctcggc gtgaatctct caacgacgcc
     1801 gctgatctcg caagtcgctc tggccctcgc gatcaaatgg ctgtcctcgg cgatttcgct
     1861 gagcagcggc tttcgcggcg gcctgttctt ctgctcgctg ctgatcggcg ccctggccgg
     1921 acgcctctat ggcgagggct tggacgcgat ctggcccgcg gcgatgatcg atccggccgc
     1981 cgcctcggtg ctcggcatgg ccgcggtcgg cgccggcgtc atcggctcgc cggtggcgat
     2041 gacgttcttc gcgctggaga cgaccaacga tttccgcgtc accgtcgccg ccctgatgtc
     2101 gagcgcgctg accgggctga tcgtgcgcga gacgttcggc tattccttcg cgacctggcg
     2161 cttccatctc cgcggcgagg cgatccgcgg cccgcaggat gtcggctgga tgcgcgacat
     2221 caaggcggcg cggctgatgc gcaaggattt ccgcgccgtc gccgccgacc gcaccatcgc
     2281 cgcagcgcgc gtgctcttcc cggtcggctc ggcgcgcgaa atcctgctga ccgacgagaa
     2341 cgaggcttac gtcggcatcg tgctggtcag cgacctgcac gccgccgcgc ataacgattc
     2401 cgagccgatc accaagctgg cgcaatggcg caacaatttc atcaccgccc agacgacgat
     2461 ccgggccgcc gtcgacgcat tcgacgaggc cgaggccgac acgctcgccg tggtcgccga
     2521 cgcccaatcg cgcaagatcg tcggcgtgct cagcgaagcg catgcgctgc gcgtctatgg
     2581 gcgagagctg gagaagcaga accaggcgtt tctgggaaga tagaggcgaa tggcgaatgg
     2641 ggaatagcga atggggggtg tagcgctatt cgccattcac cattcgccat tcgccattcg
     2701 cccttcccat tctccgcccc gccgcctaaa tctccgccat gcccgcgccg aaaatcccgc
     2761 ccgccgagcc gctcctcgac gccccgccgg ggccgacgct cgacgaggac gagagcgaag
     2821 ccggcttcga gctcgcgctc gcggaagacg aagccgcgcc gaacgcgctg gcccgcggcg
     2881 ccgcggcgat caaggcccat tggcggcagg cgccgcttgg ccccggcgtc taccggatga
     2941 tcgccgccga cggcgaggtg ctctatgtcg gcaaggcgcg cagcattaaa aagcgcatcg
     3001 ccagctatat acgcccggaa atgcaatcgg cgcgcatcgc caagatgatc gcccgcaccg
     3061 cctcgatggt cttcgtctcg accgagaccg aggccgaggc gctgctgctc gaagccaacc
     3121 tgatcaagca attgaagccg cgctacaacg tgctgctgcg cgacgacaag agctttccgt
     3181 acattctggt gacgagcggc catcgcgcgc cgcaactcgc taagcatcgc ggcgcgcgca
     3241 gcatcaaggg cgcctatttc ggcccgttcg ccagcgccgg cgcggtcggg cgcacgctca
     3301 acgcgctgga gcgcgcgttc ctgctgcgaa cctgctcgga ctcctatttc gagaatcgca
     3361 cccggccgtg ccttttgtac cagatcaagc gctgcgccgg cccgtgcacg catgaaattt
     3421 ccgacgacga ttatgcggtg ctcgttcagg aggcgaagga cttcctatcc ggcaagagcc
     3481 gcgccgtgcg cgaccgcatg gccgaggaga tgcgccaggc cgccgaggac ctagcgttcg
     3541 aaaaagcggc gcggttgcgc gaccgcatcg cggcgctctc ggctatccag ggcgagcagg
     3601 cgatcaatcc gcgcagcatc gccgaagcgg acgtgttcgc cgtcgccgag gaagcggggc
     3661 aattctgcat cgaggtgttc ttcttccgca cctaccagaa ttggggcaac cgcgcctatt
     3721 ttccgcgcgc cgaccgctcg atgagcgcgg ccgaggtgct cgattccttc ctcggccaat
     3781 tctacgcaga ccgaccggcg ccgcggctcg ttcttctgtc gcacgatatc gagagcgcgc
     3841 cgatcttgag cgaggcgctg tgcgtgaaag cgggacgacg agtcgcggtc ggccggccgc
     3901 agcgcggcga gaagcgcgaa ttggtcgaga acgccttgcg caacgcccgc gaagctctgt
     3961 cgcgcaagct gaccgaggcg gcgacgcagg aaaagctgct cggcgccctc ggcgcggcgt
     4021 tcggcttcga cgagccgatc cgtcgcgtcg acgtgttcga caattcgcac atcatgggga
     4081 ccaacgcgat cggcgcgatg gtcgtcgccg gtccggacgg cttcatgaag gcgcattacc
     4141 gcaccttcaa catcaagagc gcggagctga cgccgggcga cgattacgcg atgatgcgtg
     4201 aagtgctgac ccgccgcttc gcccgcctcg ccaaagcggc gcggcgtact gagctgaccg
     4261 aaagcggcga gacggtcccc gtcgccaagc ccgagccgga agcgggcgcc tttcccgaat
     4321 ggcccgatct cgtcctgatc gacggcggcc gcggtcagtt ggaggcggcg cgcgaggtct
     4381 tcgccatgct gcgcatcgaa ggcccgcgcc tcgtcgccat cgccaagggc cccgaccgca
     4441 acgccggccg cgagaccttc ttcatccccg gccgcgagcc cttccggctg ccgtcgcgcg
     4501 atccggcttt gtatttcgtt cagcgcttgc gcgatgaagc ccatcgcttc gccatcggca
     4561 cgcaccgcgc caagcgcaag cgcgaattcg tcaagagccc gctcgacgaa atccccggca
     4621 tcggcccggc gcgcaaacgc gctttgctca acgccttcgg cactgcgaaa gcggtggcgc
     4681 gggcgggcct ggcggatttg gagaaggtgg ctggggtgaa ccaggcgacg gcgcggattg
     4741 tgtacgaatt tttccacgag ggaggatgat tttcctcccc cgttccgctt cgcggaacag
     4801 gggaggatta gctcgcccgc gcccgttccg ccttcttgcg ctcgttcgga tcgagcagcg
     4861 ccttgcgcag gcggatcgat ttcggcgtca cttcgacgag ttcgtcgtcc tcgatatagg
     4921 cgagcgcctt ttccagcgtc atctggatcg gcggcgtcag gcgcaccgct tcgtccttgg
     4981 aatgggtgcg gatgttggtg agcttcttgc ctttgagcac attgacttcg agatcgttgt
     5041 cgcgggtgtg ctcgccgacg atcatgccct ggtacacttt ccagcccggc tcgatcatca
     5101 tcgggccgcg gtcttcgagg ttccacatcg cataggcgac ggcttcgccc gcatcgttcg
     5161 aaatcaagac gccgttgcgg cggccggcga tctcgccttt ccacggcgca taggcatgga
     5221 acagccggtt catcaccgcc gtgccgcgcg tatcggtcaa aagctcgccg agatagccga
     5281 tcagcccgcg agtcggcgcg tggaaaacga gccgcaaacg tccaccgccg gaggggcgca
     5341 tctcgaccat ctcgcctttg cgctcggaca ttttcctgac gacgattccc gaatgctcct
     5401 cgtcgacatc gatcaccact tcctcgatcg gctcgagcaa ctggccgttc tcgccgcggc
     5461 ttaagaccac tttcggccgc gaaacggcga gctcgaaccc ttcgcgccgc atcgtctcga
     5521 tcaggatcgc aagttgcaat tcgccgcggc ccgacacgac gaaagcctcg gtcgacggcg
     5581 ccggctcgac gcgaagcgcg acattgcctt ccgcctcctt gaacaggcga tcgcggatga
     5641 cccggctcgt caccttgtcg ccttctgtcc cggcgagcgg cgaatcgttg accaggaagg
     5701 tcatcgacaa agtcggcggg tcgatcggct gggcttgcag cggctcgctc acgtcgagcg
     5761 ccgccagcgt gtcggcgacg ttgaacttct ccaagccggc aatggcgacg atgtcgcccg
     5821 cctctgcctc ctcgatcggg ctgcgctcga tgccgcggaa ggcgaggatt ttcgaaatgc
     5881 ggccctgctc gatcacgttg cctt
//
LOCUS       DATGJW010000258        14650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1086520, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000258 DATGJW010000000
VERSION     DATGJW010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 14650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14650
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1086520"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..727)
                     /locus_tag="VKU03_08035"
     CDS             complement(<1..727)
                     /locus_tag="VKU03_08035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340418.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ71250.1"
                     /translation="MTAETRKLAALAAIWILLVAAPFWTPLVGGYTALGDRVLVLALA
                     AMSVNFLLGFTGVLSFGHAAYFGLGAYGAGFALKFVAPSTPLSLVCGTLLGGIVGALL
                     GLLIVRRRGVYFAMVTIAFGQVLYYIAFQWSSLTGGDDGLRGFSRQPLDLGFARIDIL
                     SNETAFYYFVLVCFALLVAIMGFVLRSPFGRAMIAIRENERRARFLGLAVERHIWIAF
                     TLSCFFIGFAGALYAHVNNFADPR"
     gene            complement(724..1593)
                     /locus_tag="VKU03_08040"
     CDS             complement(724..1593)
                     /locus_tag="VKU03_08040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ71251.1"
                     /translation="MTQLLLFNVTNGLIIGAFYVLMALGLSLILNLSNVINFAHGGFL
                     VIGGYIAYAVTPYVGFWGALLIAPPLTAVIGFFVERILIRPLYGRDPLYSLLLTFGLA
                     FMIEDGTRFIWGAQGKPVTVPAILARPLSMQFFFITGYRLFMVAVAIVVVAALFAILR
                     YTRLGLRIRAGTLDLETVAALGVNVQALRSLNFAAGVFLAGLSGVLAAGQLGLEPTMG
                     TGLLMPSFVAIIVGGVGSLTGTLIGGLLIGVASGLTAVFAPAASEAVIYVLMALILLL
                     RPRGLFGEEGMLT"
     gene            complement(1601..2863)
                     /locus_tag="VKU03_08045"
     CDS             complement(1601..2863)
                     /locus_tag="VKU03_08045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009340415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ71252.1"
                     /translation="MALVREHGRFDRRTLLKGAAALGGFAVASPAIIAARGETPIKIG
                     LVDPLTGVYAAPAQNEVMGAKLAVSRINAKGGVIGRPVELLVEDSANDVGTGVQKARK
                     LIERDEVSFIIGDVNSAIAQAISQVANEKKVLHIVSGGHTDTITGKDCKWNTYRVCHA
                     TSMEANSVCGTLFKQYGKKWHFITPDYAFGHTLQQSALADLTKLGGTMTGNELTPLGT
                     TDFSAYLIKARAANPDVILVLVQGSDMVNCLKQIVQFGLNTQVHIAGLQQELESLEAM
                     PPEARIGIWMFEWYWKQPGVPGVDQFVADIRKVNNGKVPTARHWFGYTSVQTLAEVAN
                     QQKSIDSLKLAQALANFELPPDVKLQPNKCYYRAGDHQLMSSAFIGEALAQGKDDPED
                     LFRVDKVVAGDETALPVDQTGCKVQWPA"
     gene            3167..5626
                     /locus_tag="VKU03_08050"
     CDS             3167..5626
                     /locus_tag="VKU03_08050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018517314.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLJ71253.1"
                     /translation="MPKTLHIDPTAYAKPGRLAFPEVPIRAYARSLREEQARRGDQVL
                     RDVLRHMMVVREFESMLGAFKAKGAYQDIAYAYKGPAHLSIGQEAAAVGQALGLRPED
                     HIFGSHRSHGEFIAKGLAAISRLESSDLTAIMAAHQGGALLRTVEKNLPSNSSDGLAE
                     NFLLLGLLAEIFMRANGFNAGMGGSMHAFFPPFGVYPNNAIVGASAGIATGAALWKKI
                     SGAGGVAVANSGDGSTGCGPVWEAMNFSAMAQFRTLWQDGKKGALPILFFFNNNFYAM
                     GGQTIGETMGWDRLSRIAAAANPDAMHAETVDGGDPLAVADAVERKREILLAGEGPAL
                     LDVECYRSGGHSTTDANVYRSREELAAWQAHDPIATYRAKLIEAGLMSAADAEAMQEA
                     ARAQIRAVTAAAVDPAVSPLIDIRAEPTLIGKLMFSNEEIASPEGEAALLKAPEETAR
                     VRQDAKKSRFGLTPDGEKLSPMRAVTLRDALFEAILFHMRHDERLIAYGEECREWGGA
                     FGVYRGLADILPYHRLFNSPISEAAIVATAVGYALEGGRALVELMYGDFIGRAGDEIF
                     NQMAKWRAMSGGVLKLPIVLRCSIGSKYGAQHSQDWSALVAHIPGLKVVYPATPYDAK
                     GLLASALSSEDPVVFFESQRLYDVVEQFRPGGAPADYYRLPFGEPDVKREGADLTILT
                     IGPSLYPALAAAEQLAAQSISAEVIDARSLVPFNYEAVLKSVGKTGRILLVSEASERG
                     SFPMTLAANIARFAFASLKAPPRILGAPNWIVPGAEMESTYFPQADDIVDAAAEFFPE
                     RKLARRGVRNWSDLDLARLGL"
     gene            5744..5983
                     /locus_tag="VKU03_08055"
     CDS             5744..5983
                     /locus_tag="VKU03_08055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011654708.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl domain-containing protein"
                     /protein_id="HLJ71254.1"
                     /translation="MDVPIVMPQLGNEIEEAQIDEWLKAVGDEVKAGEQILTITTPKL
                     TMEIEAPASGRLKEIKVAAEELASVGAVLGVIESA"
     gene            5989..6801
                     /locus_tag="VKU03_08060"
     CDS             5989..6801
                     /locus_tag="VKU03_08060"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLJ71255.1"
                     /translation="MASDAAAKRHDGAHPADGIAFFPLGGEGPPALLLHGFGSDHFLW
                     LANQQAIAKVADVAALDLPGHGESSMDIGAGTIGDLAERVAALLDRKAMRKVHLIGHS
                     LGGGVALVLAEQRPDLVASLVLIAPAGLGRSVDRQFLSEYSRLAAPDATEALLQRLVV
                     RPRLINKYMVGRVLGQLARPGAREALERIAHGIETCEPAVAHAAASGRLRDVPRLVLW
                     GKDDAISPLSEERLGALGGEALLVDGAGHLPHVESPRLVNEKIVEFLTRQKA"
     gene            6832..7836
                     /locus_tag="VKU03_08065"
     CDS             6832..7836
                     /locus_tag="VKU03_08065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017962183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0030246 - carbohydrate binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding transcriptional regulator"
                     /protein_id="HLJ71256.1"
                     /translation="MAKLRRAGRTPLSEAASLRLRAAWLYYSHGLTQQEVAEQLDLGR
                     TTIVRLLDEALKRGEIRIWIDEGEARCVELAVRLERALGLDEAIVTPAGKDVEQTAKS
                     VGLALGKFLSEAIADGMTIGVGWGRTLTASLQSFQPGHSQGVTIMSLLGGAVESRFAN
                     PIDYSWRLASRLGAECYLFPAPLIVDSPETKRRLIEKCGLDKLFAIAKKLDLAVVSVG
                     DIGPKASSLARHLLSEKELAELIALGCVGDVMCNFLDKDGESVAHPINQRVMSIDLET
                     VRNAGHLVIACGGAQRAEAIRAAIRRLGCNTLVTDEGAARAMLDQPLLPAKRGEGGRA
                     "
     gene            complement(7917..9182)
                     /locus_tag="VKU03_08070"
     CDS             complement(7917..9182)
                     /locus_tag="VKU03_08070"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic ring-hydroxylating dioxygenase subunit
                     alpha"
                     /protein_id="HLJ71257.1"
                     /translation="MDEKYEMRTDLRARRKGHALPRKLYLDPDYYRLDLENIWYRQWL
                     FAGHDCEIPKPGDFFTLQVGDYPVIVVRGRDGAIRAFHNTCRHRGSRICSAERGSSAR
                     LVCPYHNWSYELDGRLLFARDMGSDFDRGRHGLKAAHCESVSGYIFICLAEAAPDLSA
                     FRALIEPYVAPHALASAKLAAESTIIEEANWKLVWENNRECYHCTPNHPELCRTFPEA
                     PTISGVDGAMNDPLIADHWRRCEAIGLPSRFEISADGQYRATRMPLLRDAVSYTLSGK
                     AAVAKPLSDAIAEPNIGTLLLFHYPSIWNHLLGDHAVTFRVLPLGPTRTQVTTKWLVH
                     CDALEGVDYSVEELTHVWKATNDQDRRIVEENQRGVSSPAFEPGPYSAFHEGGVMQFV
                     EWYCQVSDDALAARPFSPLREEKVARSAG"
     gene            complement(9410..10237)
                     /locus_tag="VKU03_08075"
     CDS             complement(9410..10237)
                     /locus_tag="VKU03_08075"
                     /EC_number="4.2.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012028878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004852 - uroporphyrinogen-III synthase
                     activity [Evidence IEA];
                     GO_process: GO:0033014 - tetrapyrrole biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III synthase"
                     /protein_id="HLJ71258.1"
                     /translation="MSEELAGRRIIVPETRESALFVEMLEARGAACLACPMVAIHDAP
                     DEAPVAAWLDRFIAAPCDDLILLTGEGLRRLIALAERRGLRDAFVAALAQSRRITRGP
                     KPVRALREIGLGGDLSAEPPTSDGVIALLSRLDLKGRRIGAQLYPDSDHAALLGYLRA
                     AGASVDPILPYIYASSAEDDAVAAAIDAMAAGEVDAIAFTSAAQARRLGAVAEKTGRA
                     ESLRQALTRIVVAAIGPVAAAEIEKLGVKPDVAPAGEAYFMKPLARALAEAFARRAG"
     gene            complement(10324..11688)
                     /locus_tag="VKU03_08080"
     CDS             complement(10324..11688)
                     /locus_tag="VKU03_08080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HLJ71259.1"
                     /translation="MDGAVAVLDIGKTNVKLALFEKGRLLWEKSAPSRVLPGPPYPHE
                     DVDGAWSFLLGALREAARAHRIGTIVPTAHGAAGALIGESELAAPVMDYEFAGVEQIE
                     PDYAKLRPPFSESLSPKVPAGLNLGRQLAYQKWRCRDLFARAKHFVAFPQYWAWRLTG
                     VAASEVTSLGSHTDLWAPREGHVSSLVKALGVERLIPPLRRAFDRLGPIKPDVAAATG
                     LAPETGIFCGIHDSNASLLPYLVSRQAPFTVLSTGTWVILMSVGLSLEQLDPRDDTLA
                     NVDALGRPTATGRFMGGREYAVIAGGGGSPDRVALERVIASGAIALPCFSGQGGPYAT
                     TKGVIRGEVAEGDRPALATLYCALISDLMLTRMGATSGDLIVEGSFAGNLSFCQALGA
                     LRPKQRVFAAEDSAGTARGAAMLADWPPTYPIAAPTPVPPVIVAGLDAYRDAWTAEVD
                     LIAR"
     gene            complement(11702..12016)
                     /gene="rhaM"
                     /locus_tag="VKU03_08085"
     CDS             complement(11702..12016)
                     /gene="rhaM"
                     /locus_tag="VKU03_08085"
                     /EC_number="5.1.3.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0062192 - L-rhamnose mutarotase activity
                     [Evidence IEA];
                     GO_process: GO:0019301 - rhamnose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-rhamnose mutarotase"
                     /protein_id="HLJ71260.1"
                     /translation="MQRYAFKMFLNPGCADEYKRRHDAIWPELAALLKQSGVSNYSIH
                     LDPETNVLFAYIERSGDHRMDDLPRRKVMRRWWNRMKDIMAARPDGSPVVAPLVEMFH
                     LP"
     gene            complement(12179..13300)
                     /locus_tag="VKU03_08090"
     CDS             complement(12179..13300)
                     /locus_tag="VKU03_08090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ71261.1"
                     /translation="MKSRYLLTTAAALALAAWTTQASAAGRWDGADDLPTNPLACDAS
                     GAAAPAAAKPYDGGQPTNPPNKAGKEITVVDVPKLIGIGYFNATTKGQQEAAKELGNV
                     KVTTDGPTEAKIDEQIKFIDNYITRGVDGILFAANDPVAIAPVLKKALSKGIHVVGYD
                     ADAQPDAREWFVNQTEFNGFAKAMIDSMAKEIGEDKGFAIVTSTFTTPNQARWIAEMW
                     AYAQKCHPKLKWLETVEAQEDNNLSFNQATTLINKYGDKLSGLFGMTSVATPASAEAV
                     TKAKLCGKVAVVGLATPNAMKPYVNADCVKSVVLWNPVDLGYAAVEVMRAVVDGKLKP
                     GATSVEAGKLGKLNVVNGSEILLGPPFIFTKQNIKDFDF"
     gene            complement(13355..14326)
                     /locus_tag="VKU03_08095"
     CDS             complement(13355..14326)
                     /locus_tag="VKU03_08095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668390.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71262.1"
                     /translation="MSSAIETRVFRAPPWLKRHETILAIILVIALMGLGLLNDRFLTL
                     DNLLNQGRLMTEIGLIALPMTFVIITGGIDISVGAIVGLCAIMLGYSWKTFGFPLPLA
                     IIFCLFVGALAGFVNGLVITRVKVPPLIMTVATLALYRGLAEGVSQAHSVRGYPEWFY
                     FLGQGSVFGVPTQLLVLIVLIVISGVILDRTTFGRALYAIGANETAARFSALPVDRVK
                     LIVYTLSGLASGLAAWVLVSRVTTTRMDMGTGYELDVIAAVVLGGASIFGGSGTIWGT
                     VIGLGMIQLLKNGLALTGVKGDATIVVIGVALILSVLIASSLERRRD"
     gene            complement(14323..>14650)
                     /locus_tag="VKU03_08100"
     CDS             complement(14323..>14650)
                     /locus_tag="VKU03_08100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71263.1"
                     /translation="FALHGFFTGLASLVYATQLRVIQSTTPQGLELTIITASVVGGVS
                     ILGGVGTVVGSTLAAILIAEITSALVFVNVSPYWTDALRGALILATVIADILRRQRRA
                     AQGWKR"
BASE COUNT         2649 a         4623 c         4884 g         2494 t
ORIGIN      
        1 cgcgcggatc ggcgaaattg ttgacgtgcg catagagcgc gccggcaaag ccgatgaaga
       61 agcaggataa agtaaaggcg atccagatgt gtcgttccac ggcaaggccg aggaatcgcg
      121 cacgccgctc gttctcgcga atggcgatca tggcgcggcc gaagggcgag cgcagaacga
      181 agcccatgat ggcgacgagg agcgcgaagc agacgagcac gaaatagtag aacgccgttt
      241 cattggaaag aatgtcgatc cgggcgaagc cgagatcgag cggctggcgg gaaaagccgc
      301 gcagcccgtc gtcgccgccg gtcagcgagc tccattggaa ggcgatatag tacaaaacct
      361 ggccgaaggc gatcgtcacc atcgcgaaat agacgccgcg ccggcgcacg atcaacagac
      421 cgagcagcgc gccgacgatc ccgccgagca gcgtgccgca aacgagcgag agcggcgtgc
      481 tcggggcgac gaactttagc gcgaagcctg cgccataggc gccgaggccg aaataagcgg
      541 catggccgaa ggaaaggacg ccggtgaagc cgagcaggaa gttcaccgac atcgcggcga
      601 gcgccaggac caggacgcga tcgccgagcg cggtgtagcc gccgaccagc ggcgtccaaa
      661 acggcgccgc gacgagcaag atccaaatgg cggcgagcgc ggcgagcttt cgcgtctcgg
      721 ccgtcatgtc agcatgcctt cttcgccgaa taggccgcgc ggccggagca acaggatcag
      781 cgccatcagg acgtagatca ccgcctcgct ggcggcgggc gcgaacaccg ccgtcaagcc
      841 cgaagcgacg ccgatcagca agccgccgat caacgtcccg gtcaggctgc cgaccccgcc
      901 gacaatgatg gcgacgaagc tcggcatcaa aagcccagtc cccattgtcg gctcgagccc
      961 gagctggcct gccgccagca cgccgctgag gccggcaagg aatacgcccg ccgcgaagtt
     1021 gagcgagcgc aaggcctgga cattgacgcc gagcgccgcg acggtttcga ggtcgagcgt
     1081 gccggcgcgg atgcgcaagc cgagcctggt ataacggaga atggcgaaga gcgccgccac
     1141 gacgacaatc gcgacggcca ccatgaacag gcgatagcca gtgatgaaga agaattgcat
     1201 gctcaacggg cgggcgagaa tggcgggcac ggtgaccggc ttgccctgtg cgccccagat
     1261 gaagcgcgtc ccgtcctcga tcatgaaggc gaggccgaac gtcagcagta gactgtagag
     1321 cggatcgcgg ccatagagcg gccgaatcaa gatccgctcg acgaagaagc cgatcaccgc
     1381 tgtgagcggc ggggcgatca gcaacgcgcc ccagaagccg acataaggcg tcacggcata
     1441 ggcgatatag ccgccgatga cgagaaagcc gccatgggcg aaattgatca cgttgctcaa
     1501 attgagaata agcgacaggc caagcgccat cagcacgtag aacgcgccga tgatcaggcc
     1561 gttcgtgacg ttgaagagca ggagctgcgt catgcccctg tcaagccggc cattgcactt
     1621 tgcagccggt ctgatcgacc ggcagagccg tctcgtcgcc ggcgacgacc ttgtcgactc
     1681 ggaacaagtc ttccggatcg tccttgccct gcgccagcgc ctcgccgatg aaggccgagc
     1741 tcatcaattg atgatcgccg gcgcggtagt agcatttgtt gggctggagc ttgacgtccg
     1801 gcggcagttc gaaattggcg agagcctggg ccagcttcag cgaatcgatc gacttctgct
     1861 gattagcgac ctcggccagg gtctggaccg acgtgtagcc gaaccagtgg cgcgccgtcg
     1921 gcactttgcc gttattcacc ttgcggatat cggcgacgaa ttgatcgacg ccgggaacgc
     1981 ccggctgctt ccagtaccat tcgaacatcc agatgccgat ccgcgcttcc ggcggcatgg
     2041 cttcgagcga ttcgagctcc tgctgaaggc cggcgatgtg aacctgcgtg ttgaggccga
     2101 actggacgat ctgcttgagg caattgacca tgtccgagcc ttggaccaga acgaggatca
     2161 cgtccggatt ggcggcgcgc gccttgatca ggtacgcgga gaagtcggtc gtgccgagcg
     2221 gcgtcagctc gttgccggtc atggttccgc cgagcttagt cagatcggcg agggcggact
     2281 gctggagcgt atggccgaag gcgtagtcgg gcgtgatgaa atgccatttc ttgccgtact
     2341 gcttgaacag ggtcccgcag accgaattcg cctccatgct ggtcgcgtga cacacgcggt
     2401 acgtattcca cttgcagtcc ttgccggtga tcgtgtcggt atggccgccc gagacgatat
     2461 gaaggacctt cttttcgttg gcgacctgcg agatggcctg ggcgatggcc gaattcacgt
     2521 cgccgatgat gaagctgacc tcgtcgcgct cgatcagctt gcgcgccttc tgcacgccag
     2581 tgccgacgtc gttcgccgaa tcctcgacga gcagttcgac cggccggccg atcacgccgc
     2641 ctttggcgtt gatccgcgag acggcgagct tggcgcccat cacttcgttc tgcgccggcg
     2701 cggcgtagac gcccgttaaa ggatcgacca ggccgatctt gatcggggtt tcgccgcgcg
     2761 cggcgatgat cgcgggagaa gcgacggcga agccgcccaa agcggcggcg cctttgagga
     2821 gagttcgacg atcgaaccgg ccgtgttcgc gcaccaacgc catgatattc ctcccgccgt
     2881 ttcttgatct tttgtcgggc gagacgccct gcgccgcccg attcgatatt gcgcctgaaa
     2941 ttgtcgcgct cgcaagggtt gcgcgggcgg cgcgtccatt cggccagccg cagcggtgcg
     3001 ggccggactg gcggctgcga ggcgcgccgg cgtcacctgc ggcgctgatg tgcggccatt
     3061 tggcgcaatt gttcgttagt cgtagccgac cttgatttta aacatttgat cgcctagaaa
     3121 tacaaatgtc acaagatcgt cgtggcgacc tcttttcgga ttggctatgc cgaaaacgct
     3181 tcatattgac ccgaccgcct acgcaaaacc gggcaggctc gctttccccg aagtgcccat
     3241 ccgggcttat gcgcgttctt tgcgcgagga acaggcgagg cggggcgacc aggtcctccg
     3301 cgacgtcctc cgtcacatga tggtggtgcg ggaatttgaa tcgatgctcg gcgctttcaa
     3361 ggccaagggc gcataccagg acatcgccta cgcttataag ggccccgcgc atctttcgat
     3421 cggccaggag gccgccgcgg tcggccaagc gctcggcttg cggcccgaag atcacatttt
     3481 cggcagccat cgcagccatg gcgagttcat cgccaaaggc ctcgctgcga tttcgcggct
     3541 cgaatcttct gatcttacgg cgattatggc tgcgcatcag ggcggagcct tgcttcgtac
     3601 cgtcgaaaag aacctgccga gcaattcgtc cgatggtctt gccgagaatt ttctcctgct
     3661 tgggctgctc gccgagattt ttatgcgggc gaacggcttc aacgccggca tgggcggcag
     3721 catgcatgct ttcttcccgc ctttcggcgt ctatccgaat aatgcgattg tcggcgcctc
     3781 ggcgggcatt gcgaccggcg cggcgctttg gaagaagatc agcggcgccg gcggcgtcgc
     3841 cgtcgccaat agcggcgacg gctcgaccgg ttgcggcccc gtctgggagg cgatgaattt
     3901 ttccgccatg gcgcagttcc gtacgctgtg gcaggacggc aagaagggcg cgctgccgat
     3961 cctcttcttc ttcaacaaca atttctacgc gatgggcggc cagacgatcg gcgagacgat
     4021 gggttgggat cgcctgtcgc gcatcgccgc cgcggccaat cctgacgcca tgcatgccga
     4081 aacagtggac ggcggcgatc cgctcgccgt cgccgacgcg gtcgagcgca agcgcgaaat
     4141 cctgctcgcc ggcgaagggc cggcgctgct cgatgtcgaa tgctatcggt cgggcggcca
     4201 ttcgaccacc gacgccaatg tctatcgcag cagggaggag cttgcggcct ggcaggcgca
     4261 tgatccgatt gcgacctatc gcgcgaaact gatcgaggcg ggtctgatga gcgcggccga
     4321 tgcggaggcg atgcaggaag ccgcgcgcgc gcaaatccgc gccgtgaccg ccgccgcggt
     4381 ggatcccgcg gtctcgccgc tgatcgatat ccgcgccgag ccgacgctga tcggcaagct
     4441 gatgttctcg aacgaagaga tcgcctcgcc ggaaggcgag gcggcgctgt tgaaagcgcc
     4501 ggaagaaaca gcccgcgtcc gccaggacgc caagaagagc cgtttcggct tgacgccgga
     4561 cggcgaaaag ctttcgccga tgcgcgccgt cactctgcgg gacgcgctgt tcgaggcgat
     4621 cctttttcac atgcgccatg acgagcggct gatcgcctat ggcgaggagt gccgcgaatg
     4681 gggcggcgct ttcggggtct atcggggcct cgcggacatc ctgccgtacc atcgcctgtt
     4741 caattcgccg atatccgagg cggcgatcgt cgccacggct gtcggctacg cgctcgaagg
     4801 cggccgggcg ctggtcgagt tgatgtatgg cgatttcatc ggccgcgccg gcgatgaaat
     4861 cttcaaccag atggcgaaat ggcgggccat gtcgggcggc gttctcaagc tgccgatcgt
     4921 gctgcgctgc tcgatcggca gcaaatacgg cgcccagcat tcgcaggatt ggtcggctct
     4981 ggtcgcccat attccgggcc tgaaggtcgt ctacccggcg acgccctatg acgcgaaagg
     5041 cctgctcgcc tcggctctct ccagcgagga tccggtcgtc tttttcgaga gccagcggct
     5101 ctacgacgtg gtcgagcaat tcaggccggg cggcgcgccc gcggactact atcgcctgcc
     5161 gttcggcgag ccggacgtga agcgggaagg ggcggatttg acgatcctga cgatcggccc
     5221 ctcgctgtac ccggcgcttg cggcggccga acaactcgcg gcgcaatcca tctcggccga
     5281 ggtgatcgac gcgcgttcgc tcgttccgtt caattacgaa gccgtgctga aatcggtcgg
     5341 caagaccggc cgcatcctgt tggtttcgga agcgtcggag cgcggcagtt tcccgatgac
     5401 gctcgccgcc aatatcgcgc gcttcgcttt cgcctccctc aaagcgccgc cgcgcatcct
     5461 cggcgcgccg aactggatcg tgcccggcgc cgaaatggaa tcgacgtatt ttccgcaagc
     5521 cgacgatatt gtcgatgcgg cggcggagtt ctttccggag agaaagctcg cgcggcgcgg
     5581 cgtgcgcaat tggagcgact tggaccttgc tcggctcggg ctttgagaac gcgttgcgac
     5641 gttctctgcg gcggatgctc agccgccgcg ctgtagccgc ggtcgtcgac cgcggctaca
     5701 aatacgccgg accacgccat tgccgctcgt gaaaggacaa atcatggacg tgccgattgt
     5761 gatgccgcag ctcgggaacg agatcgagga ggcgcagatc gacgaatggc tcaaagccgt
     5821 cggcgacgag gtcaaggccg gcgagcaaat cctgacgatc accacgccca agctgaccat
     5881 ggagatcgaa gcgccggcaa gcggccgcct taaggagatc aaagtggcgg ccgaggagct
     5941 cgcctcggtc ggcgcggttc tcggcgtgat cgaaagcgcg tgaagccgat ggcgtccgac
     6001 gcggcggcca agcggcacga cggcgctcac cctgctgatg ggatcgcctt ttttccgcta
     6061 ggcggagaag ggccgcccgc cttgctcttg cacggcttcg gctccgacca ttttttatgg
     6121 ctcgccaatc agcaggccat cgcgaaagtg gccgatgtcg cggcgctcga tctgccgggg
     6181 catggcgaat cttcgatgga cattggcgca gggacgattg gcgatctcgc ggagcgggtc
     6241 gcggcgctcc tcgatcgaaa ggcgatgcgc aaggtccatc tgatcggcca ttcgcttggc
     6301 ggcggcgtcg cgctcgtctt ggccgagcag cgcccggacc tcgtcgcttc gctcgtcttg
     6361 atcgcgccgg ctgggctcgg ccgcagcgtc gaccgtcagt tcctctcgga atattcgcgg
     6421 ctcgccgcgc cggacgcgac tgaggctctg ttgcaacgcc ttgttgtcag gccgcgtctc
     6481 atcaacaaat acatggtcgg ccgcgtcctc ggccaattgg cgcggcccgg cgcgcgcgaa
     6541 gcgctcgagc ggatcgcgca cgggatcgaa acgtgcgaac cggcggtcgc gcatgcggcg
     6601 gcttcgggcc gcctgcgcga cgtcccgcgc cttgtccttt ggggcaagga cgacgcgatc
     6661 agtcctttgt cggaagagcg gctcggcgcg cttggcggcg aggctctgct cgtcgatggc
     6721 gcgggtcacc tgccccacgt cgagtcgcct cggctcgtca atgaaaagat cgtcgaattt
     6781 ctcacccggc aaaaagcatg atggcggccg gcccaaccgg cgcgaagacc catggctaag
     6841 ttgcgccgcg ccggccgcac gccgctctcg gaagccgcat cgctcaggct gcgcgcggcg
     6901 tggctttatt acagccatgg cctgacgcag caggaagtcg cagagcagct cgacctcggc
     6961 cgcaccacca tcgtgcggct gctcgacgaa gcgctgaaac gcggcgaaat ccgcatctgg
     7021 atcgacgagg gcgaggcgcg ctgcgtcgaa ctcgctgtgc gattggagcg cgcgcttggg
     7081 ctcgacgagg cgatcgtcac gccggccggc aaggacgtcg agcaaaccgc gaaatcggtc
     7141 ggcctagcgc tcggaaaatt cctgtcagaa gcgatcgcag acggcatgac gatcggcgtc
     7201 ggctgggggc gaacgctcac cgcctcattg caaagcttcc agccgggcca cagccagggc
     7261 gtcacgatca tgtcgctgct tggcggcgcc gtcgagagcc gcttcgccaa tccgatcgac
     7321 tattcctggc gcctggcgag ccggctcggc gccgaatgct atctttttcc ggcgccgctg
     7381 atcgtcgatt cgcccgagac caagcggcgg ctgatcgaga aatgcggcct cgacaagctc
     7441 ttcgccatcg ccaagaagct ggatctcgcc gtggtcagcg tcggcgatat cggaccgaaa
     7501 gcctcctcgc tcgcccggca tttattgtcg gagaaagagc ttgccgaact tatcgccctc
     7561 ggctgcgtcg gcgacgtgat gtgcaacttt ctcgacaagg acggcgagag cgtcgcccat
     7621 ccgatcaatc agcgagtcat gtcgatcgat ctcgaaacgg tccgcaacgc cggccatctg
     7681 gtcatcgcct gcggcggcgc gcaaagggcc gaggcgatcc gcgcggcgat caggcgcctc
     7741 ggctgcaata cgctggtcac ggatgaagga gccgcgcggg cgatgctgga ccagcccctt
     7801 ctccctgcaa agcggggaga aggcggccgc gcgtagcacg gccggatgag ggcagcgcgg
     7861 agcctgatta gctagcgttt cgaagggcac gtaccctttg agtcctgtgc tgcctctcat
     7921 ccggcgcttc gcgccacctt ctcctcgcga agcggggaga agggacgcgc cgccagcgcg
     7981 tcgtcgctga cctggcagta ccattccacg aattgcatca cgccgccttc gtggaaggcg
     8041 gagtacggcc cgggctcgaa ggcgggcgag gagacgccgc gctgattctc ctcgacgatg
     8101 cgccggtcct gatcgttcgt ggctttccag acatgagtca gctcctcgac cgaatagtcg
     8161 acgccttcga gggcgtcgca atggacgagc catttcgtcg tcacttgcgt ccttgtcggt
     8221 ccgagcggaa ggacgcggaa cgtgacggcg tggtcgccga gcaggtggtt ccagatcgac
     8281 ggataatgga agagcagcag ggttccgata ttcggttcgg cgatcgcgtc gctcaacggc
     8341 ttggccacgg cggccttgcc cgacagcgta tagctgacgg cgtcgcggag cagcggcatg
     8401 cgcgtcgcgc gatattggcc gtccgccgaa atttcgaacc ggctcggcag gccgatcgct
     8461 tcgcagcgcc gccaatggtc ggcgatgagc ggatcgttca tcgcgccgtc gacgccgctg
     8521 atcgtcggcg cttccggaaa ggtgcggcag agctcaggat ggttgggcgt gcaatgatag
     8581 cactcgcggt tgttctccca gaccagcttc caattggcct cctcgatgat cgtgctctca
     8641 gccgcgagct tggcgctggc gagggcgtgc ggggcgacat aaggctcgat cagcgcgcgg
     8701 aaggcggaaa ggtccggcgc cgcctcggcg aggcagatga aaatatagcc gctgacgctt
     8761 tcgcaatgcg cggctttcaa gccatgccgg ccgcggtcga aatcggagcc catgtcgcgc
     8821 gcgaagagaa ggcgtccgtc gagttcgtag ctccaattgt ggtaagggca gaccagacgg
     8881 gcggaggaac ctctttccgc cgagcagatg cgcgagccgc gatggcggca ggtattgtga
     8941 aaggcgcgga tcgctccgtc cctgccgcga acgacgatga cgggatagtc gccgacctgc
     9001 agcgtgaaga aatcgccggg tttcgggatt tcgcaatcat ggccggcgaa cagccattgg
     9061 cgataccaga tgttctcgag atcgagccgg tagtaatcgg gatcaagata gagctttctg
     9121 ggcagcgcgt gtccctttct gcgggcgcgg agatcggtcc tcatctcgta cttttcgtcc
     9181 ataacattcc tcgcgggagc gcgtttaaac gccggctaca taacttagca ttgccgcccg
     9241 caaggcgcca ggacaaccct cgccccggga aagggtggtc gcgaagcggc cgggtgaggg
     9301 gctgcgccga ggtatgcgat gagcgatacg gtgcgtacct tgcgtcctgc gcagctcttc
     9361 atccggcgct tcgcgcgacc cttttcccgc gaagcgggga gaagggctcc taccccgcgc
     9421 gccgcgcgaa agcttcggcc agggcgcggg cgagcggctt catgaaatag gcctcgccgg
     9481 ccggcgcgac gtccggcttg acgccgagct tctcgatctc cgccgcggcg accgggccga
     9541 tcgcggcgac gacgatgcgc gttaacgcct ggcgcaagct ctcggcgcgt cccgtcttct
     9601 cggccacggc gccgaggcgg cgcgcctgcg ccgcgctggt gaaggcgatg gcgtcgacct
     9661 cgccggcggc catcgcgtcg atcgccgcgg cgacggcgtc gtcttcggcg ctcgacgcat
     9721 agatgtaagg caggatcgga tcgacgctcg cgcccgccgc gcggagatac ccgagcagcg
     9781 ccgcatggtc gctgtccgga tagagctgcg cgccgatccg gcgccccttc agatcgaggc
     9841 gcgacaaaag ggcgatcacg ccgtcgctgg tcggcggctc ggccgagaga tcgccgccga
     9901 ggccgatctc ccgcagcgcg cgcaccggct tcgggccgcg cgtgatccgg cgcgattggg
     9961 cgagcgccgc gacgaaggcg tcgcgaagcc cacgccgctc cgccagcgcg atcagccggc
    10021 gcaagccttc gccggtcaga aggatcaggt cgtcgcaagg cgcggcgatg aagcggtcca
    10081 gccacgcggc gaccggcgcc tcgtccggcg catcgtggat agcgaccatc gggcaggcga
    10141 gacatgccgc gccgcgcgcc tccaacatct cgacgaagag cgccgattcc cgagtttcgg
    10201 ggacgatgat gcgccgcccg gccaattctt cgctcatgca gcctctctca acattgacga
    10261 aaattctcgc gcctttcgcg agcagagcgc cgaaggccga ggcgttcccg ttcggcgtcc
    10321 ggctcagcga gcgatgaggt cgacctccgc ggtccaagcg tcgcgatagg cgtccaggcc
    10381 cgcgacgata accggcggta cgggcgtcgg cgcggcgatc ggataggtcg gcggccagtc
    10441 ggcgagcata gcggcgccgc gcgccgtgcc ggcggaatct tcggcggcga agacgcgctg
    10501 cttggggcgc agcgcgccga gcgcctggca aaagctgaga ttgccggcga agctcccctc
    10561 gacgatgaga tcgccgctcg tcgcgcccat gcgggtcagc atcaggtcgc tgatcagggc
    10621 gcagtaaagc gtcgccaaag ccgggcggtc gccttccgcg acctcgcccc ggatgacgcc
    10681 tttggtcgtc gcgtaaggcc cgccctggcc cgaaaagcag ggcagtgcga tcgcgccgga
    10741 ggcgatgacg cgctccaagg cgacgcggtc gggactgccg cccccgccag cgatgacggc
    10801 gtattctcga ccgcccatga accgcccggt cgcggtcggc cggccgagcg cgtcgacatt
    10861 ggcgagcgtg tcgtcgcgcg gatcgagctg ctcgagcgac agcccgacgc tcatcagaat
    10921 gacccaggtc ccggtcgaca gtacggtgaa tggagcttgg cgcgagacga ggtagggcag
    10981 gagcgaggcg ttggaatcgt gaatgccgca gaaaatcccg gtttccggcg cgaggccggt
    11041 cgcggcggcg acgtccggct tgatcggacc gagtcgatca aaggcgcggc gcagcggcgg
    11101 aatcaggcgc tcgacgccga gcgccttcac caggctcgag acgtggcctt cgcgcggcgc
    11161 ccaaagatcg gtatggctgc cgagcgaggt cacttcggac gcggcgacgc cggtgagacg
    11221 ccacgcccaa tattgcggaa atgcgacgaa atgtttggcc cgcgcgaaga gatcgcgaca
    11281 gcgccatttc tgataagcga gctggcggcc gagattgagg ccggccggca ctttcggcga
    11341 caggctttcc gaaaaaggcg gccgcaattt cgcgtaatcg ggctcgatct gctcaacgcc
    11401 ggcaaactca tagtccatga ccggcgcggc gagttcgctc tcgccgatca acgcgcccgc
    11461 cgcgccatgc gcggtcggca cgatcgtccc gatccgatgc gcccgcgccg cctcgcggag
    11521 cgcgccgagc aggaacgacc aggcgccgtc gacatcttca tgcggataag gcgggcccgg
    11581 cagaacgcgg cttggcgccg atttctccca aaggagccgg cctttctcga acaaagccag
    11641 cttgacattg gtcttgccga tatcgaggac cgcgaccgct ccgtccatcg cttctaagtc
    11701 ctcacggcag gtgaaacatt tcgacgagcg gcgccacgac gggcgaaccg tccggcctgg
    11761 ccgccatgat gtccttcata cgattccacc agcgccgcat gaccttgcgg cgcggcaggt
    11821 cgtccatgcg atgatcgccg cttcgctcga tataggcgaa gaggacattc gtctccgggt
    11881 cgagatggat ggaatagttc gaaacgccgc tttgcttcag cagggccgca agctccggcc
    11941 agatcgcgtc gtggcggcgc ttatactcgt cggcgcagcc gggattgaga aacatcttga
    12001 aagcgtagcg ttgcatcgcg ggcggctcgc tatagaccgc gttttctcca accatcattg
    12061 cgagcgaagc gaagcaatcc gttccaaggg cggcgcccta tgaatggatt gcttcgccac
    12121 tgcgctctcg caatgacgga aagtcccggc cgcgctacgc ggccgggaca ggagtcgatc
    12181 agaagtcgaa gtccttgatg ttctgcttgg tgaagatgaa gggaggtcca agcaggattt
    12241 cgctgccgtt gacgacgttc agcttgccga gcttgccggc ttccaccgag gtcgcgccgg
    12301 gcttcagctt gccatcgacg acggcgcgca tcacttcaac cgcggcgtag ccgagatcga
    12361 ccgggttcca cagcacgacc gacttgacgc aatcggcgtt gacatagggc ttcatcgcgt
    12421 tgggcgtggc gagcccgacc accgcgacct tgccgcaaag cttggccttg gtcacggctt
    12481 ccgccgaagc cggcgtcgcg accgacgtca tgccgaacag gccggagagc ttgtcgccgt
    12541 atttgttgat cagcgtcgtc gcctggttga aggacaagtt attgtcctcc tgcgcttcga
    12601 ccgtctcgag ccatttcagc ttgggatggc acttctgcgc ataggcccac atctcggcga
    12661 tccagcgcgc ctggttgggc gtcgtgaagg tcgaggtgac gatggcgaag cccttgtcct
    12721 cgccgatctc cttggccatc gaatcgatca tcgccttggc gaagccgttg aactcggtct
    12781 ggttgacgaa ccattcgcgc gcatcgggct gggcgtcggc gtcatagccg acgacgtgga
    12841 tgcctttcga cagcgctttc ttaaggaccg gcgcgatggc tacggggtcg ttggcggcga
    12901 acaggatgcc gtcgacgccg cgggtgatgt agttgtcgat gaacttgatc tgctcgtcga
    12961 tcttggcttc ggttgggccg tcagtcgtga ccttgacatt gccgagctcc ttggccgcct
    13021 cctgctggcc cttggtcgtc gcgttgaagt agccgatgcc gatcagcttc ggcacatcga
    13081 cgacggtgat ttccttgccg gccttgttcg gcggattggt cggctgcccg ccgtcgtaag
    13141 gtttagccgc cgctggggcc gcagcgccgc tcgcgtcgca ggcgagcggg ttcgtcggca
    13201 gatcatcggc gccgtcccat ctgcctgcgg cgctggcctg cgtcgtccag gccgcgagcg
    13261 cgagagccgc cgccgtggtc aacagatatc tcgatttcat gttcgcttcc ttccctcaca
    13321 cggcccacgg gagcggaccg atttctagtg tttgtcaatc ccggcggcgc tcgagcgagc
    13381 tcgcgatgag caccgatagg atgagcgcga cgccgatgac cacgattgtt gcgtcgccct
    13441 tgacccccgt caacgccaga ccgttcttca agagctggat catccccaag ccgatcaccg
    13501 tgccccagat ggtgccggag ccgccaaaga tgctggcgcc gccgaggacg accgcggcga
    13561 tgacgtcgag ctcgtagccg gtccccatat ccatccgcgt ggtcgtcacc cgcgagacca
    13621 ggacccaggc ggcgaggcct gaggccaagc cggaaagcgt ataaacgatg agctttaccc
    13681 gatcgaccgg cagcgcggag aagcgcgccg ccgtctcatt ggcgccgatc gcgtagagcg
    13741 cgcggccaaa cgtcgtacgg tcgagtatga cgcccgagat gacgatgaga acgatgagaa
    13801 ccaggagttg cgttggcacg ccgaagacgc tgccctgacc taaaaaatag aaccattccg
    13861 gatagcctcg caccgaatgc gcctggctca cgccttcggc gagaccgcga tagagcgcga
    13921 gtgtcgcgac cgtcatgatg agcggcggca ccttcacccg ggtgatgacg aggccgttga
    13981 cgaagcccgc aagcgcgccg acgaagagac agaagatgat cgccagcggc agcggaaagc
    14041 cgaacgtctt ccacgagtag cccagcatga tcgcgcacaa gcccacgatc gcgccaaccg
    14101 aaatgtcgat gccgccggtg atgatcacga aagtcatcgg cagcgcgatg aggccgatct
    14161 cggtcatgag ccggccctgg ttcaagagat tgtcgagggt gagaaagcgg tcgttcaaaa
    14221 ggccgaggcc catgagcgcg atcaccagga tgatcgcgag tatggtctcg tggcgcttga
    14281 gccaaggcgg ggcgcgaaag acgcgggttt cgatggccga actcatcgct tccacccctg
    14341 tgcggcgcgg cgctggcggc gcaggatgtc ggcgatgacg gtggcgagga tgagcgcgcc
    14401 gcgcaaggcg tccgtccagt agggcgagac gttgacgaac acgagcgcgc tcgtgatctc
    14461 ggcgatgaga atcgccgcca gcgtcgagcc gacgaccgtg ccgacgccgc ccagaatgct
    14521 gacgccaccg accaccgagg cggtgatgat ggtgagttcg agaccctgcg gggtcgtcga
    14581 ctggatcacc cggagctgcg ttgcatagac gagcgaagca agcccggtga agaagccatg
    14641 cagcgcgaac
//
LOCUS       DATGJW010000259        10957 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_463210, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000259 DATGJW010000000
VERSION     DATGJW010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 10957)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10957)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10957
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463210"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..323
                     /locus_tag="VKU03_08105"
     CDS             <1..323
                     /locus_tag="VKU03_08105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLJ71264.1"
                     /translation="PVGSAQPQARVVRIMEKIAQALKNRPGAVIVRGHTDARPYRSAT
                     YDNWRLSSARAQMAYYMLVRGGLPEKRVERIEGYADRRLKLPNNPTAAENRRIEILLR
                     RPKP"
     gene            320..1495
                     /locus_tag="VKU03_08110"
     CDS             320..1495
                     /locus_tag="VKU03_08110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71265.1"
                     /translation="MTARRLVIAFAGLLMSLGAARAAETSSTPLKMLADLQNLQAKIA
                     QGDKAAYAAQPTMLHDMGAAIAAADPDTWKQPDNIHAAIAFLLSGGPPRAVASLVEMQ
                     GMAKDDEQLLHGALAYVVGREEEAEKLLGGFDARTLDLRFAGQIAFVQSSLIAAKDKK
                     KAIELLDLARLLAPGGLVEEAALRREILMAGEMRDVDRFALLSSQYLSRFPKSIYADN
                     LIRGVAATAARLGLAEDLTDFHKFNSLVASLPAEARRGFYLTISRAALIDGKIEVARV
                     AARMSLAQAYGDLADETRGKFYAAAAVVLGGQYDDGVAELKAIDASNLPRRDVSLLLA
                     AKDVAARLHATPPAPRELPAVAPSGDLAKSDEAAMATIRMAEAAVARSDGLVKEKFR"
     gene            1492..2973
                     /locus_tag="VKU03_08115"
     CDS             1492..2973
                     /locus_tag="VKU03_08115"
                     /inference="COORDINATES: protein motif:HMM:NF014207.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook-length control protein FliK"
                     /protein_id="HLJ71266.1"
                     /translation="MSASSVAPQAAAATANAAPAGLMAARAAQHGGAAGQSVFAAVLQ
                     ELASDAQSPAPEGVKSSKDNAPKSGAAKDGAARDKPDDPPEAKTGQSADAAAAIAALV
                     APQPDAATSAAAVAALIAPRLAANASAAATASAAPAASPPLSNGMARAAAAPRSDIQA
                     NAPTPGAAATQAWAARLQAQGQPAAAAGALMPLMPSDSTAQLALAAPPSLAPPVVDVH
                     VQRARTYLGVDGATQAAKVDSVALASAGSDPAKNAKPSPSAAALAAPPAKANGGEQAG
                     DSAMRREGSNDARNGAGDQNSREIPTAATSAPAVSDPGLVSGAVAAAPVSLDQLPDLI
                     AQQAETMTAPAAAANTSAPAASQNPVKELEVQLNPADLGSLTVKMRLANGNLAVVIET
                     SKDSTAKLIEGERDAIAERLASVEQPVASVVIQASDSVPNQGGDNNGPGSATPQQGDA
                     QSSAANSSNGQAHSAPRDDRPARQANPAEDEASRRARSGDLFV"
     gene            3017..3484
                     /locus_tag="VKU03_08120"
     CDS             3017..3484
                     /locus_tag="VKU03_08120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLJ71267.1"
                     /translation="MTRASRLNDIPLNVLYAVGLTETGHGGELSPYDMNVDGRAVRSK
                     SLAEALAKFAAERANGAKFIDIGCMQINERFHRADFASLSDMFDPAQNVRYAAAFLKQ
                     LKAQEGSWTLAVARYNAGPNNNAAQKKYVCAVIGNMVASGLGRWTTNARNFCN"
     gene            complement(3602..4057)
                     /locus_tag="VKU03_08125"
     CDS             complement(3602..4057)
                     /locus_tag="VKU03_08125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71268.1"
                     /translation="MIKLVLIGAWASFATLASGYAAGYLKTAYAKSGAEQPAGPVEAK
                     KTKEIDVPVIRNGALKGYIVTQFVYSVNAAAPKKLSIFPEALVVDEAFRYLFDDETID
                     LENLKKSDLKKLTNTIIKNVNARLKNDVVADLAIQEFTFLTAAEVKQRF"
     gene            complement(4054..4464)
                     /locus_tag="VKU03_08130"
     CDS             complement(4054..4464)
                     /locus_tag="VKU03_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein FlgN"
                     /protein_id="HLJ71269.1"
                     /translation="MHAPSRGPTIVTLAATRAARACEMILPVIDRLQQTLDAENEQIA
                     SGKRIDYEALNQRKSQGLLELTRLAPMIAGAEASKPLSDALAALRAKLEANQRMLRIQ
                     LNAARKVADIIAKAIQDGQSDGTYSAYAWQEPDE"
     gene            complement(4467..5063)
                     /locus_tag="VKU03_08135"
     CDS             complement(4467..5063)
                     /locus_tag="VKU03_08135"
                     /inference="COORDINATES: protein motif:HMM:NF021638.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod-binding protein"
                     /protein_id="HLJ71270.1"
                     /translation="MAINPPSDIVLDVLNAADPGRAEAAAQRLGALAGASPAAAAADF
                     AKALDAARRSSAGGLAGGVGMADARIKLLKKDAAAAQKAKNAQIEFEATLLNNLVGDM
                     LPKDAAGVFGQGTAGDVWRSMLGDQIAHQIAKSGELGIAKRLFATHPLARENGGAHAS
                     LVAPGGGANVAQASANPLSLPSGADYSDGAFLFTKPKT"
     gene            complement(5114..5509)
                     /locus_tag="VKU03_08140"
     CDS             complement(5114..5509)
                     /locus_tag="VKU03_08140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71271.1"
                     /translation="MKKRLDSLEKISQLQSKLHDLTRWRLAAIGRQRSSLEEAERAMI
                     EALNSEAVGHGAPAAAAAGRLRSISRQIAAAKADYDAQSQRVLDQGTRAKLAQRLVES
                     AGANYRADKERKDLGDLIERSLGKKTSSA"
     gene            complement(5519..6307)
                     /locus_tag="VKU03_08145"
     CDS             complement(5519..6307)
                     /locus_tag="VKU03_08145"
                     /inference="COORDINATES: protein motif:HMM:NF013478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006605 - protein targeting [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthetic protein FliR"
                     /protein_id="HLJ71272.1"
                     /translation="MNEAADFLGGAHGVLGSHLALGVFILFCRIGGCLMLAPGFSNAQ
                     IPTQVRLFIALAATLALTPLLIGRLPLQKLSDEPLPALRMIGTELLIGGSIGLMARIF
                     MIALETMAVASATGLGLANPFGVEMEANDALPPLATFVTLSATALIFVLDLHWEVLRG
                     LVASYSAVPVSDTFDPGFALRHIGDILVETFRAALRICSPFILYAIVVNLAISIINRL
                     TPQIAIFYISAPFVIAGGLALLFFTIKSMLGEFMASFSAWLVSG"
     gene            complement(6307..8397)
                     /gene="flhA"
                     /locus_tag="VKU03_08150"
     CDS             complement(6307..8397)
                     /gene="flhA"
                     /locus_tag="VKU03_08150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020084256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FlhA"
                     /protein_id="HLJ71273.1"
                     /translation="MAVSTIAAPAPVGRSGGKDVGFAIGIVLILCIFFVPVPAVCIDL
                     GLALSIALSVLILMVALWIQKPLEFSAFPTVLLVATILRLALNISTTRLILSHGAQGT
                     TAAGYIIGGFANLVMGGDFVIGIIVFLILVTINFLVITKGATRIAEVGARFTLDAIPG
                     KQMAIDADLNAGLIDEKVAQARRRELEEESSFFGSMDGASKFVRGDAIAGLIILAVNI
                     FGGVVIGVTRHGLTLASAADIFTKLSVGDGLVSQIPALIISLAAGMLVAKGGTRGSAE
                     KAVLGQLSNYPNALFLAALLMFILGVIPGLPFLPFAILGGLMAFAGVAIPQRLAKDRA
                     AAAAAQAASEERARAEAKSSIKEQLKTPEVELCLSKELTTKLLASHQEIGSRVAKMRR
                     KFARQYGFVVPEIKLTDDIKAPPKTYQFRIHGTVVATQELRLNEYLVIVGDGKRPNVP
                     GEEVREPAFGIKALAISEAFVAHAKREGFKTVDPISVLLTHLSEIIRNNLAQLLSYKD
                     MRALLDRLDPEYKRLIDEIIPSQISYSGLQAVLKLLLAERVSIRNLHLILEAIAEIAP
                     HARRPEQIAEHVRMRIAQQICGDLLDHGALKVLRLGNRWDLVFHQALKRDAKGDVVEF
                     DIDPRLMEQFGGEASSAIRARMDQGHHFALVATAETRPYVRMIIERMFATLPVLSQLE
                     IARGFDVRSLGTIS"
     gene            complement(8473..8745)
                     /gene="fliQ"
                     /locus_tag="VKU03_08155"
     CDS             complement(8473..8745)
                     /gene="fliQ"
                     /locus_tag="VKU03_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469393.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis protein FliQ"
                     /protein_id="HLJ71274.1"
                     /translation="MNEADALDLVQSAIWTIIVGAGPAVGAAMAVGIVIALMQALTQV
                     QEMTLTFVPKIIVVFVVISLTAPFIGAQFYAFTEGVYSRIEKGYGP"
     gene            complement(8742..9146)
                     /gene="flgD"
                     /locus_tag="VKU03_08160"
     CDS             complement(8742..9146)
                     /gene="flgD"
                     /locus_tag="VKU03_08160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006204068.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar hook assembly protein FlgD"
                     /protein_id="HLJ71275.1"
                     /translation="MTVSATTPISSATSSTAASSSTPTLSYNSFLTLLTTELKNQDPT
                     QPMDPTQMVTQLATISQVGQAVQTNSTLSSLLTATSLSQAEQLIGKTITSADGTTSGQ
                     VASVSVTSSGSTATLTNGATVSIGSGVTVAAQ"
     gene            complement(9160..9570)
                     /gene="flbT"
                     /locus_tag="VKU03_08165"
     CDS             complement(9160..9570)
                     /gene="flbT"
                     /locus_tag="VKU03_08165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963530.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0048027 - mRNA 5'-UTR binding [Evidence
                     IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA];
                     GO_process: GO:1902209 - negative regulation of
                     bacterial-type flagellum assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis repressor FlbT"
                     /protein_id="HLJ71276.1"
                     /translation="MNISLRADEKLYINGAVIRVDRKVTIELLNDVTFLLENHVMQAN
                     EATTPLKQIYFVVQIMLMDPSATPGAMTLSRKLIDAALTAFETPEIIAGLRTVDEMIK
                     RSRNFDAMKTLRGLFPLEQAVVFPSAQPAAPHAA"
     gene            complement(9583..9930)
                     /gene="flaF"
                     /locus_tag="VKU03_08170"
     CDS             complement(9583..9930)
                     /gene="flaF"
                     /locus_tag="VKU03_08170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0044781 - bacterial-type flagellum
                     organization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar biosynthesis regulator FlaF"
                     /protein_id="HLJ71277.1"
                     /translation="MYQFSYAEVIDDSPREMRARERQAMDRAIELLRAAQAGGPGSHE
                     TFEALHYLRRLWSIFLDDLNNPDNELPPQLRAGLISIGIWINKEIERVRTGKTTDLTP
                     MIEINEIIRGGLT"
     gene            complement(9946..>10957)
                     /locus_tag="VKU03_08175"
     CDS             complement(9946..>10957)
                     /locus_tag="VKU03_08175"
                     /inference="COORDINATES: protein motif:HMM:NF004669.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="flagellar hook-associated family protein"
                     /protein_id="HLJ71278.1"
                     /translation="LTVAQTQSQLATATTESSTGQYADLGLQLGEQSGYELSLKNHNN
                     LLQALTDANNIVSTNLSTTQSTLDAIRSNAQSTLQSLTQWGTGTGAGSTLQSLGVSSL
                     QSLISAANTTSAGQYIFGGINSNVAPMADYFSTPTSQAKTDIDNAFQSAFGTSPNGAG
                     ASSITASQMQQFLDNQFASLFQGSSWSSDWSSASSTNVSNQIAPGETIDTGTNANQPG
                     FQQLAQAFAMLTEFGGTSLSQAAQQAVASTASSLITSALGSLTQTEATVGEAQSRITD
                     ANNSMSSQMTILQTQIGNLDNVDSYKVATEVSTLTTQLQAAYQLTAQLQQLSLAKYLP
                     VA"
BASE COUNT         1999 a         3409 c         3636 g         1913 t
ORIGIN      
        1 tcccagtcgg ctcggcccag ccgcaagcgc gcgtcgtacg catcatggag aaaatcgctc
       61 aagcgctgaa gaaccggccg ggcgcggtca tcgtgcgcgg ccataccgac gcgcgcccct
      121 atcgctccgc gacctacgac aattggcggc tttcgtcggc gcgcgcgcaa atggcctatt
      181 acatgctggt gcgcggcggc ctgccggaga aacgggtcga gcggatcgaa ggttacgccg
      241 accggcgctt gaagctcccg aacaatccga ccgcggcgga aaatcggcgc atcgaaattc
      301 tgctgcgcag gccgaaacca tgaccgcccg gcgcctcgtc atcgcctttg ccggcctgtt
      361 gatgtcgctc ggcgccgcgc gcgccgccga aacgtcttcg acgccgctta aaatgctcgc
      421 cgatctgcaa aacctgcaag ccaaaatcgc gcaaggcgac aaggccgcct atgccgcgca
      481 gccgacaatg ctgcacgaca tgggcgccgc catcgccgcg gcggacccgg acacatggaa
      541 acagccggac aatatccacg ccgcgatcgc cttcctgctc agcggcggtc cgccgcgcgc
      601 cgttgcgtcg cttgtggaaa tgcagggaat ggcgaaggac gacgagcaat tgctgcacgg
      661 cgcgctcgcc tatgtcgtcg ggcgggagga agaggcggaa aagctgctgg gcggcttcga
      721 cgcccggacg ctcgacctgc gcttcgccgg gcagatcgcc ttcgtgcaat cttcgctgat
      781 cgcggcgaag gacaagaaga aggctatcga actgctcgac ctggcgcggc tcttggcgcc
      841 tggcggcctg gtcgaagaag cggcgctgcg gcgggaaatc ctgatggctg gcgaaatgcg
      901 cgatgtcgac cggttcgcgc tgctctccag ccagtatcta agccgttttc cgaaatcgat
      961 ctacgccgac aatctcatcc gcggcgtcgc cgcgacggcg gctcgcctcg gccttgccga
     1021 ggacctgacc gattttcata agttcaacag tctcgtcgcc tccctgcctg ccgaggcgcg
     1081 gcgcggcttc tatctcacaa tttcccgcgc ggcgcttatc gacggcaaaa tcgaggtcgc
     1141 ccgcgtcgcc gcgcgcatgt ctctggcgca agcttatggc gatcttgccg acgagacccg
     1201 cggcaagttt tatgccgcgg cggcggtcgt cctcggcggc cagtacgacg acggcgtcgc
     1261 cgaactcaaa gcgatcgatg cgagcaattt gccgcgacgc gacgtttcgt tgctgctggc
     1321 ggcgaaggac gtcgccgcgc gcttgcacgc gaccccgccc gcgccaagag aattgccggc
     1381 ggtcgctccg agcggcgatt tggcgaaaag cgacgaggcg gcgatggcca cgattcgcat
     1441 ggctgaagca gcggtggcgc gcagcgacgg actggtgaag gagaagttcc gatgagcgcg
     1501 tcaagcgttg cgccgcaagc ggcggccgcg acggcaaacg ccgctcccgc cgggctgatg
     1561 gcggcgcgcg ccgctcaaca cggcggcgcc gccggtcagt cggtcttcgc cgccgtgctt
     1621 caggaactgg cgagcgacgc gcaatcgccc gcgcccgaag gcgtcaaaag cagcaaggat
     1681 aatgcgccga aatccggcgc cgccaaggac ggcgccgctc gcgataagcc ggacgacccg
     1741 ccggaagcca agaccggaca gagcgccgac gccgccgcgg cgatcgccgc tctcgtcgcg
     1801 ccgcagcctg acgccgcgac gagcgctgcg gcggtcgccg cgctgattgc gccgcgattg
     1861 gccgcgaatg cgagcgccgc cgcgacggcg agcgccgctc cggccgcttc gccgccgctc
     1921 agcaacggaa tggcgagggc ggccgccgct ccgcgcagcg acatccaggc gaacgcgccg
     1981 acgcccggcg cggcggccac gcaggcatgg gcggcgcgcc tgcaggcgca aggccagccg
     2041 gcggccgccg cgggcgcgct catgccgctc atgccgtcgg attcgacagc gcaattggcc
     2101 ctggcggcgc cgccaagcct cgcccctccc gtcgtcgatg tccatgtcca gcgcgcgcga
     2161 acctatctgg gcgtcgacgg cgcgacgcag gcggccaagg tcgattccgt cgcgcttgcg
     2221 agcgccggct cggaccccgc gaagaatgca aagccgtcgc cttcggcggc ggcgctcgcc
     2281 gcgcctcccg ccaaggcgaa tggcggcgag caagcgggcg acagcgcgat gcggcgcgag
     2341 ggatcgaacg acgcccggaa tggcgccggc gatcaaaatt cccgcgaaat tcctacggcg
     2401 gcgacaagcg cgccggcggt gagcgatcct ggcctggtca gcggcgccgt cgccgccgcg
     2461 cccgtcagcc tcgaccaatt gccggatctc atcgcccagc aggccgaaac catgaccgcg
     2521 cccgcggccg cggcgaatac aagcgcgccg gccgccagtc agaatccggt caaagagctc
     2581 gaagtgcaac tcaacccggc cgacctcggc tcgctcaccg tcaaaatgcg gctcgccaac
     2641 ggcaatctgg ccgtggtcat cgagacctcc aaggacagta cggcgaaatt gatcgaaggc
     2701 gagcgcgacg ccatcgccga gcgtctggcc tcggtcgaac agccggtggc gtccgtggtg
     2761 attcaagctt ccgacagcgt cccaaatcag ggcggcgaca acaatgggcc aggttcagca
     2821 actccgcagc aaggcgatgc gcaaagcagc gcggcgaaca gttcgaacgg acaagcgcat
     2881 tccgcgccgc gcgacgacag gccggcgcgt caggcgaatc ctgcggaaga cgaagcgtct
     2941 cgccgcgccc gttctggcga tctttttgtc tagcctggcg gcggctcgcg ccgacgattt
     3001 ggcttgtgaa agagagatga cgcgcgcgtc gcggctgaac gacattcccc tcaacgtgct
     3061 ttacgccgtc gggctaacgg aaaccggtca tggcggcgag ctcagcccct acgacatgaa
     3121 cgtcgacggg cgcgcggtcc ggtcgaagag cctcgccgaa gcgctggcca aattcgccgc
     3181 cgagcgcgcc aacggcgcca agttcatcga tatcggctgc atgcagatca acgaacgctt
     3241 ccaccgcgcc gacttcgcct cgctgagcga catgttcgat ccggcgcaga acgttcgcta
     3301 cgcggcggcg tttctcaagc agctcaaggc ccaggaagga agctggacat tggccgtcgc
     3361 ccgctacaat gccgggccga acaacaatgc ggcgcaaaag aaatatgttt gcgcggtgat
     3421 cggcaatatg gtcgccagcg gcctcggccg atggacgacg aatgcccgga atttctgcaa
     3481 ttaggcaggc gttttgccgc cattgcggaa aagcgaagcg acgaagcaaa tccattcgta
     3541 aggctcagcc ctatgaatgg attgcttcgc tccgctcgca aagaccggcg ggtcgggcga
     3601 tctagaagcg ctgcttgacc tcggcggccg tcaggaaggt aaattcctga atcgccagat
     3661 cggcgacgac gtcgtttttc aggcgcgcgt tgacattctt gatgatcgta ttggtgagtt
     3721 tcttcaggtc ggatttcttg agattttcga gatcgatcgt ctcatcgtcg aaaagataac
     3781 ggaacgcctc gtcgaccacc aaagcctcgg ggaagatgct gagcttcttc ggcgcggcgg
     3841 cgttgaccga gtagacgaac tgcgtgacga tatagccctt gagcgcgccg ttgcggatga
     3901 cgggtacgtc gatctccttc gtcttcttgg cttcgaccgg cccggccggc tgctcggcgc
     3961 cgctcttggc gtaagccgtc ttgagatagc ccgccgcata gccggaggcg agcgtggcga
     4021 aagacgccca ggcgccgatc aggacgagct tgatcattcg tcgggctcct gccatgcata
     4081 ggccgaatag gtgccgtccg actgtccgtc ctgaatcgcc ttggcgataa tgtcggcgac
     4141 cttgcgcgcg gcgttcaact gaatgcgcag catgcgctga ttggcttcga gtttcgcgcg
     4201 caaggcggcc agagcgtcgc tcaacggctt gctcgcctca gcgccggcga tcatcggcgc
     4261 aagccgcgtc agttcgagaa gaccttggct tttgcgctgg ttgagcgctt cgtagtcgat
     4321 acgcttgccg ctggcgatct gctcgttctc ggcgtcgagc gtttgctgca aacggtcgat
     4381 aaccggcagg atcatctcgc aggcgcgagc agctcgcgtg gcggcgagcg tgacgatcgt
     4441 tgggccgcgg gagggagcgt gcatgatcaa gtcttcggct tcgtaaagag aaaggcgccg
     4501 tcgctgtaat cggcgccgct gggcagggaa agcggattgg cgctggcttg agcaacattg
     4561 gcgccgccgc cgggggcgac cagcgaagca tgagcgccgc cattttcccg cgccagagga
     4621 tgcgtggcga agagccgctt ggcgatgccg agctcgccgg atttggcgat ctggtgggcg
     4681 atctggtcgc cgagcatcga ccgccagacg tcgcccgccg ttccttggcc gaagacgccg
     4741 gccgcatcct tgggcagcat atcgccgacc aggttgttca gcagcgtggc ttcgaattcg
     4801 atctgcgcat tcttcgcctt ttgtgctgcg gcggcgtcct tcttcagcag cttgatgcgc
     4861 gcgtcggcca tgccgacgcc gccggcgaga ccgcccgccg aggatcgtcg cgcggcgtcg
     4921 agcgccttgg cgaaatccgc agccgccgca gccggcgacg cgccggccaa ggcgccgagc
     4981 cgctgcgccg ccgcttccgc gcgtccggga tcggcggcgt tcaaaacatc aaggacaatg
     5041 tccgagggag gattgatggc cacgacggct tttccaaaag agaatccctt taaagcagag
     5101 tccctcagca ggcttacgcc gagcttgtct tcttgccgag cgagcgctcg atcaggtcgc
     5161 cgaggtcttt gcgctccttg tcggcgcggt aattcgcgcc ggcgctttcg acaaggcgct
     5221 gcgcgagctt ggcgcgggtc ccctggtcga gaacgcgttg cgactgggcg tcgtaatcgg
     5281 ctttggcggc ggcgatctgc cggctgatcg agcgaaggcg cccggccgcg gccgcggccg
     5341 gcgcgccatg cccgacggct tcgctgttga gggcttcgat catggcgcgc tcggcctctt
     5401 cgagcgacga gcgctgccgt ccgatcgcgg ccagccgcca gcgcgtcagg tcgtgcagct
     5461 tggactgcaa ctgcgatatc ttctccaacg aatccagacg ctttttcatt ttttaggact
     5521 accccgacac gagccacgcc gagaagctcg ccatgaactc cccaagcatc gatttgatcg
     5581 taaagaaaag caaagccagg ccgccggcga tgacgaatgg cgcggatatg tagaaaatcg
     5641 cgatctgcgg cgtcaggcga ttgatgatgc tgatcgccag attgacgacg atcgcataga
     5701 ggataaaggg cgagcagatg cgcaaagccg cccggaatgt ctcgacaaga atatcgccga
     5761 tatggcgcag cgcgaaaccg ggatcgaacg tgtccgacac cggcaccgcc gaataggagg
     5821 cgacgagccc gcgcaaaacc tcccagtgca gatcgaggac gaagatcagc gccgtcgccg
     5881 acaaagtgac gaacgtggcg agcggcggca gcgcatcgtt cgcctccatt tcgacgccga
     5941 acggattggc gaggccgagg ccggtggcgc tggcgaccgc catcgtttcg agcgcgatca
     6001 tgaaaatgcg cgccatcagg ccgatcgagc cgccgatcag cagctcggtc ccgatcattc
     6061 gcaaagcggg cagcggctcg tcgctcaatt tctgcaaggg tagccggccg atcagcaggg
     6121 gcgtcaaggc caacgtggcg gcgagcgcga tgaacaggcg cacctgggtc ggaatctgcg
     6181 cgttggagaa tcccggcgcc agcatcaggc agccgccgat ccggcagaaa aggatgaaca
     6241 cgccgagcgc cagatgcgat ccgaggacgc catgagctcc gccgagaaaa tccgccgcct
     6301 cgttcatcac gaaatcgtgc cgagcgatct cacgtcgaag cctcgcgcga tttcgagctg
     6361 cgaaaggacg gggagcgtgg cgaacatcct ttcgatgatc atgcgcacat aagggcgcgt
     6421 ctcggcggtc gcaacgagag cgaaatggtg tccctgatcc atgcgggcgc ggatcgccga
     6481 cgaggcctcg ccgccgaact gctccatcag gcgcggatcg atgtcgaatt cgaccacgtc
     6541 gcccttggcg tcgcgtttta aggcctggtg gaagaccaag tcccagcgat tgccgagccg
     6601 gagcactttc agcgcgccat gatcgagaag gtcgccgcaa atctgctgcg cgatgcgcat
     6661 gcgcacatgc tcggcgatct gctccgggcg gcgcgcatgc ggcgcgattt ccgcgatcgc
     6721 ttcgaggatg aggtgcaggt tgcggatcga tacgcgctcg gccagcagaa gcttcagaac
     6781 ggcttgcagg ccggaatagg aaatctgcga cggaatgatc tcatcgatca gccgtttgta
     6841 ttcgggatcg agccggtcga gaagcgcccg catgtccttg tacgagagga gctgcgccag
     6901 attgttgcgg atgatctcgc tgagatgggt gagcagaacc gagatcggat cgaccgtctt
     6961 aaagccctcc cgcttggcgt gggcgacaaa ggcttccgat atggcgagcg ccttgatgcc
     7021 gaaggccggc tcgcgcacct cttcgcccgg cacgttcggg cgcttgccgt cgccgacaat
     7081 caccaggtat tcgttcaagc gcagctcttg cgtcgccacg accgtgccgt gaatgcggaa
     7141 ttgataggtc ttcggcggcg ccttgatgtc gtcggtcagc ttgatttcgg ggacgacgaa
     7201 accgtattgg cgcgcgaatt tgcggcgcat cttggcgacg cgcgagccaa tctcctggtg
     7261 cgaggccaag agtttggtcg tcagctcctt gctcaggcag agctcgactt ccggcgtctt
     7321 caactgctcc ttgatcgacg acttcgcctc cgcccgcgcc cgctcctcgc tcgccgcctg
     7381 ggccgccgca gccgcggcgc gatctttggc gaggcgctgc gggatggcga cgccggcgaa
     7441 agccatcaac ccgccgagaa tcgcgaaggg caggaacggc aatcccggga tcacgccgag
     7501 gatgaacatc agcagcgcgg cgagaaacag cgcgttcgga taattgctga gttggccgag
     7561 caccgccttt tcggccgagc cgcgcgtgcc gcccttggcg accagcatgc cggcggcgag
     7621 cgagatgatc agcgccggga tttgcgagac gaggccgtcg ccgaccgaca gcttggtgaa
     7681 aatatcggcg gcgctggcca gcgtcaggcc gtggcgcgtc acgccgatga cgacgccgcc
     7741 gaaaatattg acggcgagga tgatcaggcc ggcgatcgcg tcgccccgca cgaatttcga
     7801 ggcgccgtcc atcgagccga agaaggagct ttcctcctcc aattcgcggc ggcgggcctg
     7861 ggcgaccttc tcgtcgataa ggccggcgtt gaggtcggcg tcgatcgcca tctgcttgcc
     7921 gggaatggcg tcgagagtga agcgggcgcc cacttccgcg atgcgggtcg cacctttggt
     7981 gatgacgaga aaattgatcg tcaccaggat caggaacacg atgatgccga tgacgaaatc
     8041 gccgcccatg acgagattgg cgaagccgcc gatgatgtag ccggccgccg ttgtgccttg
     8101 cgcgccatgc gacaggatga ggcgggtcgt cgagatgttg agcgccagcc gtaggatcgt
     8161 cgcaaccagc agaacggtcg gaaaggctga gaattccaac ggcttttgga tccacagcgc
     8221 gaccatcagg atcaggaccg agagcgcgat ggacagcgcc aagccgagat cgatgcagac
     8281 ggcgggaacc ggaacgaaga agatgcacag gatgaggacg atgccgatcg cgaaaccgac
     8341 atccttgccg ccgctgcggc cgaccggcgc cggcgcggct atcgtcgaga ccgccatccc
     8401 ttctccttca tcctggcttg gagaaagagt gcagggttaa gcttgtgcga atctcgccag
     8461 cgcgccgtgc gcttaaggcc cgtaaccttt ttcgatgcgg ctatagacgc cttcggtgaa
     8521 ggcatagaac tgcgcgccga taaaaggcgc ggtgaggctg atcacgacga agacgacgat
     8581 gatcttcggc acgaaggtga gcgtcatctc ctgcacttgg gtcagcgcct gcatgagggc
     8641 gatcacgatg ccgaccgcca tcgcggcgcc gacggccggg ccggccccga cgatgatcgt
     8701 ccagatcgcg gattgaacga ggtcgagcgc gtccgcttcg ttcattgcgc ggcgaccgtc
     8761 acgccgctgc cgatcgaaac cgtggcgcca ttggtcagcg tcgcggtcga gcccgaactc
     8821 gtcacgctga ccgaggcgac ctggccggag gtcgtcccat ccgccgaggt gatcgtcttg
     8881 ccgatcaact gctcggcttg cgacagcgac gtcgccgtga gcagcgacga cagggtgctg
     8941 ttcgtttgca ccgcctggcc gacttgcgag atcgttgcaa gctgcgtgac catctgggtc
     9001 gggtccatgg gctgggtggg atcctggttc ttcagctcgg tcgtcaacag cgtcaagaac
     9061 gaattgtagc tgagcgtcgg cgtcgaggag ctcgcagcgg tcgagctggt cgcgctcgaa
     9121 atcggcgttg tggcgctaac ggtcattggc ctaatccttc taggcggcgt ggggagcggc
     9181 gggctgggcg ctcggaaaca ccaccgcctg ttcgagcgga aacagtccgc gcagcgtctt
     9241 catggcgtcg aaattgcgcg agcgcttgat catttcgtcg acggttctca ggcctgcgat
     9301 gatttccggc gtctcaaaag cggtcaaggc ggcgtcgatc agcttgcgcg acagcgtcat
     9361 agcgcccggg gtcgccgacg ggtccatcag catgatctgg acgacgaaat aaatctgctt
     9421 gagcggcgtc gtcgcctcat tcgcctgcat gacgtgattt tcgagaagaa acgtcacgtc
     9481 gttgagcaac tcgatcgtga ccttgcggtc gacgcgaatc acggcgccgt tgatataaag
     9541 tttttcgtca gcgcgaagcg agatgttcat ggtccttcgc tcctaggtca agccgccgcg
     9601 gatgatttcg ttgatctcga tcatcggcgt caggtcggtc gtcttgccgg tgcgcacgcg
     9661 ctcgatttcc ttgttgatcc agatgccgat cgagatcagc cccgcccgca attgtggcgg
     9721 cagctcgttg tcgggattgt tgaggtcgtc gaggaaaatc gaccacaggc ggcgcagata
     9781 gtggagcgcc tcgaaagtct catggctgcc cggccccccg gcctgggcgg cgcgcaacag
     9841 ctcgatcgcc cgatccatgg cctggcgctc gcgggcgcgc atctcgcgcg gcgagtcgtc
     9901 gatgacttct gcgtaggaaa attgatacat ctctggtcct gcggcttaag cgacggggag
     9961 atatttggcg aggctgagct gctgtaattg agcggtgagc tgataggcgg cctgaagctg
    10021 cgtggtcagc gtactgacct ccgtcgccac cttgtaggaa tcgacattgt cgagattgcc
    10081 gatctgggtc tgcagaatgg tcatttgcga ggacatcgaa ttgttggcgt cggtgatccg
    10141 cgactgcgcc tcgccgaccg ttgcctcggt ctgggtcaga gagcccagcg cgctggtgat
    10201 caaggaggag gccgtcgacg cgaccgcttg ctgcgcggcc tggctgagcg aggtgccgcc
    10261 gaattcggtc agcatcgcga aggcttgggc gagctgctgg aaaccgggct gattggcgtt
    10321 cgttcctgtg tcgatcgtct cgccgggcgc gatttggttc gaaacattgg tgctcgaagc
    10381 ggacgaccag tcgctgctcc aggacgagcc ttgaaacagc gaggcgaact ggttgtcgag
    10441 aaattgctgc atctgcgacg cggtgatcga gcttgcgcca gcgccgtttg ggcttgtgcc
    10501 gaaggcgctc tggaaggcgt tgtcgatatc ggttttggct tgcgatgtcg gggtcgaaaa
    10561 ataatcggcc attggggcga cgttggaatt gatgccgccg aatatgtact ggccggccga
    10621 cgttgtgttc gccgctgaga tcagtgattg cagcgagctg acgccgaggc tctgcaatgt
    10681 gctgcccgcg ccggttcccg ttccccattg ggtcaggctt tgcaacgtgc tttgggcgtt
    10741 gctgcgaatg gcgtcgagcg tcgactgggt ggtggagaga ttggtcgaga caatgttgtt
    10801 cgcgtcggtc aacgcctgca gcaggttgtt atggtttttc agcgaaagct catagcccga
    10861 ctgctcgccg agctggaggc cgagatcggc atattggccg gtggaggatt ccgtcgtcgc
    10921 agtggcgagc tgcgactgcg tctgcgccac cgtgagc
//
LOCUS       DATGJW010000260         7795 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1187388, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000260 DATGJW010000000
VERSION     DATGJW010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7795)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7795)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7795
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187388"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..605)
                     /locus_tag="VKU03_08180"
     CDS             complement(153..605)
                     /locus_tag="VKU03_08180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006200368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2214 family protein"
                     /protein_id="HLJ71279.1"
                     /translation="MFTDWVLACLHHIAVFSLAATLAAELALMSVDLSARSIRRLAAI
                     DIWYGIVAAAVILFGVMRVVWGAKGYEYYIVNYVFWTKMALFLAVGLLSIGPTLRYGA
                     WRRKLSAEAAFLPPTAEVARVRAFLWLETALFLCIPIAAAAMARGYGF"
     gene            complement(622..3756)
                     /locus_tag="VKU03_08185"
     CDS             complement(622..3756)
                     /locus_tag="VKU03_08185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter permease subunit"
                     /protein_id="HLJ71280.1"
                     /translation="MGSFNLSLWAIRRPALTLFMILALGLGGALAYLQLGRAEDPSFT
                     IKNVNVSAIWPGATTEEMQNQVTDRIEKKLRELAYFDKLTTYTKPGFASIGVDFKDST
                     PAHLVPMLFLDLRKKLGDIRGDLPTGLIGPNVNDEFGDVDSVLYAITGDGATYAQLKA
                     VAEALRLRLQRVPDVTKVDLFGDQGERIYVEFSHAKLATLGVPVQALFDSLAKQNAVT
                     PAGEFQTDGQRIPLRVTGALHGVEAVKETPVFAGGRTFRLGDIAAVKHGYEDPPSYLI
                     RSQGQPALEIGVVMQRGANILALGKNLDAALADFEQALPQGVTITRIADQPKVVAAAV
                     FEFMRSFVEALIIVLAVSFLSLGWRSGIVVATSVPLVLAIVFIVMAFIGLDLHRITLG
                     ALIIALGLLVDDAIIATEMMVVKMEQGWPRERAGSFAWTSTAMPMLTGTLVTAIGFLP
                     IGLANSAVGEYAGGIFWVVGIALIASWFVAVIFTPYLGVKLLPSYAGRKAHGDPDAIY
                     DTRFYRAFRRALEFCVGRPSVVILGVLGLLAAGLTAFTHVQQQFFPISERPELFFEIR
                     MPEGTSIAATSQAAREGEKLLTGDSDALSYTTYIGKGSPRFWLGLLPVQPNEAFAQIV
                     IVAKDIAARERLKARIDKAVAAGALSAARVRVDRFNFGPPVGFPVQFRVIGPDPEKAR
                     AIAYRVRDIMRTDPRVIDPNLDWNEKMPSVTLEVDQARARALGLTPQDLAQTLQTLIG
                     GVTVTTIRKGEEKVDVVARAVPSERAGLADLDSLTILSHDGLAVPLSQVAHIKYGSEE
                     PIIWRRNRDVAVTARADVVDGAQAPDVTNALWPKLQGVRDSLEPGYRLEIGGAIEESQ
                     KGNSSIFVLFPIMGLGMLALLMLQLQSFSRLAVVFFTAPLGIIGASVALLLADRPFGF
                     VALLGLIALAGMDMRNTVILVDQIEKDVRELGVTRREAIVSATVRRARPVALTALAAI
                     LAMIPLSESAFWGPMAVTIMGGLFVATFLTLFFLPAMYALWFRKSLGERGTRQTAAQP
                     SFGFAPGLAPTAAE"
     gene            complement(3756..4835)
                     /locus_tag="VKU03_08190"
     CDS             complement(3756..4835)
                     /locus_tag="VKU03_08190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HLJ71281.1"
                     /translation="MRLRSVGVVCVSLILAGCNAHGEVKREAPPRPVLVAQIHYAPRV
                     ADRVLPGVVKARIESDLGFRIAGKIARRLVDSGAVVKKGQKLAELDDSDLKLQEEQAD
                     ADLAAAKSAREQQDADLARVETLHRQGWSAAADLDKIRAAADQARANLVKAERAATLA
                     GDALSYATLTADADGIVTAALAEPGQVVAAGAPVVRLAHSGEKEAAVAIPEALVERAK
                     SAPAEAEFWALPGLTLQALLRELSPSADPTTRTYEARYSLVDAPASVALGMSASVKLA
                     LDSEKVARAPLGAILDQGQGPMVFIVDEASGAVTPANVKVVAYDSESVLIAGGAPEGA
                     LIVALGAHKLDPTQKVRVVRHIAGL"
     gene            complement(4849..5502)
                     /locus_tag="VKU03_08195"
     CDS             complement(4849..5502)
                     /locus_tag="VKU03_08195"
                     /inference="COORDINATES: protein motif:HMM:NF037152.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR family transcriptional regulator"
                     /protein_id="HLJ71282.1"
                     /translation="MILALRISPDESRERILVAAEELFRRIGYYKTTVGDIAQALGMS
                     SANIYRFFPSKNAIVEALCAKLLGGLLEAATAAADAPGAPSERVFNLLMTLHRHHRDH
                     LTNQERVHEMVVVAMDENWGAIDAFLESCKGLVAKLIGEGQAAGEFGPGEPAQLADLV
                     FGACASILHPTMIAKCAGEGRTVEEIDLLAQGLVAFALRALASRQPLPQFRPCSFAP"
     gene            complement(5662..7338)
                     /locus_tag="VKU03_08200"
     CDS             complement(5662..7338)
                     /locus_tag="VKU03_08200"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HLJ71283.1"
                     /translation="MMSKTAIYWAAGLGVVAVAAVTPAILKFYDIGWPGAAAAPPALS
                     QKAAESGDREAAAPAAPLKAPESAAAEAPAKPAEPAASSAPPVAAPSAAPPPKGEAAA
                     SPGGSQPAPGERAASAAAPPSASPPPPAAASAAPAAAPAPSSPAAAGPQQATPSTPTT
                     HATQAAPPAKAEAQRPAFDVVRVEPSGDAVIAGRASPRAAVQLRSDGRVVAQVDADEM
                     GQFAILPPPFPAGDHRLQLAARSGGAEVLSDAIGIQVPATAESARQNAPQSPEKQAAS
                     AQNPVEPQHLAEAAAPKAGAPAAPKATPAPEPPKVAALGAEPSKQAASAPLVAILSVE
                     TNGVGRFEAKGAAEPNALVRLYLNNAFVAEAKAGADGRWSLTVERGMTPGPYAIRADE
                     IDRANGAVVARAEVPFDYPARPTGEAAAAAAPPAADGAVKPADQTASAKPAEEIAAAP
                     ASPQAAEAAPRPAGAEPPRQAQSLAASTPSATPAPAAAPAKQDGANPVVPEIRTTKVV
                     RGDNLWNLSKRFYGYGPRYKVIYQANASQIRNPRLIYPDQIFVVPRNPPQ"
     gene            7604..>7795
                     /gene="rplU"
                     /locus_tag="VKU03_08205"
     CDS             7604..>7795
                     /gene="rplU"
                     /locus_tag="VKU03_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HLJ71284.1"
                     /translation="MFAVIKTGGKQYKVAAEDEITVMTLAGEPGETVTFGEVLALFDG
                     DTNHVGAPHIEGASVAGTIV"
BASE COUNT         1303 a         2542 c         2761 g         1189 t
ORIGIN      
        1 agcgccggat gaggggctgc gcaggactgg aaaggtacgc accctacgag acgcgagtca
       61 caacgttcgg cgctgcccct caccggctgc ttcgcggcca ccctctcccc gctgcgcggg
      121 gcgagggggt gcgcacgtgg cgagggtgga atctagaaac cgtacccccg cgccatcgcc
      181 gccgccgcga tagggatgca caggaacagc gccgtttcca gccacagaaa cgcccgcacg
      241 cgcgcgactt ccgccgtcgg cggcaggaag gcggcttccg cgctcagctt gcggcgccat
      301 gcgccatagc ggagggtcgg accgatcgag agcaggccga cggcgaggaa caaagccatc
      361 ttcgtccaga agacgtaatt gacgatgtaa tattcatatc ccttggcgcc ccagacgacc
      421 cgcatgacgc cgaagagaat caccgccgca gcgacgattc cgtaccagat gtcgatcgcg
      481 gcgaggcggc ggatcgatcg cgcgctcaag tcgacgctca tcaaggccag ttcggccgcg
      541 agcgtcgcgg cgagcgagaa gaccgcgatg tggtgcaggc aggcgagaac ccagtccgtg
      601 aacattggct tcaaaatcct atcattccgc cgctgtcggc gcgaggcccg gggcaaaacc
      661 gaacgagggc tgggccgccg tttgccgcgt cccgcgctcg ccgagcgact tgcggaacca
      721 cagcgcgtac attgccggta ggaagaacag cgtcaggaat gtcgcgacga acaggccgcc
      781 catgatcgtc accgccatcg gcccccagaa ggcgctctcg gagagcggga tcatggcgag
      841 gatcgcggcg agcgcggtga gcgcgacggg acgcgcgcgc cggaccgtcg ccgagacgat
      901 cgcctcgcgc cgcgtcacgc cgagctcgcg cacgtccttc tcgatctggt cgaccagaat
      961 caccgtgttg cgcatgtcca tgccggcgag agcgatcagg ccgagcagag cgacgaagcc
     1021 gaacggccgg tccgccagga gcagcgcgac ggaggcgccg ataatgccga gcggcgcggt
     1081 gaagaagacc acggcgaggc gcgagaagct ctgcaattgg agcatcagca gcgcgagcat
     1141 gcctaagccc atgatcggga agaggacgaa gatcgaggaa ttgcctttct gcgattcctc
     1201 gatcgcgccg ccgatttcga ggcggtagcc cggctctagg ctgtcgcgga cgccctgcag
     1261 cttcggccag agcgcgttgg tcacgtccgg cgcctgcgcg ccgtcgacca catcggcgcg
     1321 agcggtgacc gcgacgtcgc ggttgcgccg ccagatgatc ggctcctccg agccgtattt
     1381 gatatgcgcc acttgcgaga gcggcacggc gagaccgtca tgcgagagga tggtcaggct
     1441 gtcgagatcg gcgagtccgg cccgttcgga cgggacggcg cgcgcgacga catcgacctt
     1501 ctcctcgccc ttgcggatcg tcgtcacggt gacgccgccg atcagggtct gcaaagtctg
     1561 ggcgaggtct tgcggcgtca ggccgagcgc gcgggcgcgc gcctgatcga cttcgagcgt
     1621 caccgacggc attttctcgt tccaatcgag attgggatcg atcacgcgcg gatcggtacg
     1681 catgatgtcg cgcacacgat aggcgatcgc gcgcgccttt tcggggtccg ggccgatgac
     1741 gcggaactgg acggggaagc cgaccggcgg gccgaaattg aaacggtcga cgcgcacccg
     1801 cgccgccgac agtgcgcccg ccgccaccgc cttgtcgatc ctggccttca ggcgctcgcg
     1861 cgccgcgatg tccttggcga caatcacgat ctgcgcgaaa gcttcgttcg gctgcaccgg
     1921 caagagacca agccagaagc gcggcgagcc cttgccgata taggtcgtgt aggaaagggc
     1981 gtcggaatcg ccggtgagca gcttttcgcc ttcgcgcgcc gcttgcgacg tcgctgcgat
     2041 tgacgttccc tccggcatgc gaatttcgaa gaacaattcc ggccgctcgg aaattggaaa
     2101 gaattgctgc tggacatggg tgaaggccgt caggccggcc gcgagcaagc caagcactcc
     2161 aagaatcacc acagacggcc gcccgacgca gaattcgagg gcgcggcgga aggcgcgata
     2221 gaagcgcgtg tcgtagatcg cgtcaggatc gccatgagct ttgcggccgg catagctcgg
     2281 caggagcttg acgccgagat agggcgtgaa gatcacggcg acgaaccacg aggcgatcag
     2341 cgcgatgccg acgacccaga aaatgccgcc ggcgtactcg ccgacggcgg aattggcgag
     2401 gccgatcggc aggaagccga tcgcggtcac cagagtgccg gtcagcatcg gcatggcggt
     2461 cgaggtccag gcgaaggagc cggcgcgctc gcgcggccag ccctgctcca ttttcaccac
     2521 catcatctcg gtggcgatga tcgcgtcgtc gacgagaaga ccgagcgcga tgatcaaggc
     2581 gcccagcgtg atgcggtgaa ggtcgaggcc gataaacgcc atgacgatga agacgatggc
     2641 gaggacgagc ggcacggagg tcgcgacgac gatccctgag cgccagccga gcgagaggaa
     2701 gctgacggca agaacgatga tcagcgcctc gacgaaggag cgcatgaact cgaacaccgc
     2761 cgcagccacg actttcggct ggtcggcgat gcgcgtgatg gtcaccccct gcggcagcgc
     2821 ctgctcgaaa tcggcgagcg ccgcatcgag attcttgccc aaggccagaa tattcgcgcc
     2881 gcgctgcatg acgacgccga tttcgagcgc cggctggcct tgcgagcgga tcaggtacga
     2941 aggcgggtcc tcgtagccat gcttgaccgc ggcaatgtcg ccgagacgaa acgtgcggcc
     3001 tccggcgaag acgggggtct cttttaccgc ctcgacgccg tgcagcgcgc cggtgacgcg
     3061 cagagggatg cgctggccgt cggtctggaa ttcgccggcc ggcgtcaccg cgttctgctt
     3121 ggcgagcgaa tcgaacaggg cctggaccgg cacgccgagc gtcgccagct tggcgtggct
     3181 gaactcgaca tagatgcgct cgccctgatc gccgaagaga tcgaccttgg tcacgtccgg
     3241 cacgcgctgc aggcgcaggc gcaaagcttc ggcgaccgct ttcagttgcg cataggtcgc
     3301 gccgtcgccg gtgatcgcat agaggaccga atcgacatcg ccgaattcgt cgttgacatt
     3361 cggcccgatc aggcccgtgg ggagatcgcc gcggatgtcg ccgagcttct tgcgcaggtc
     3421 gagaaacagc atcggcacga gatgggcggg cgtcgaatcc ttgaaatcga cgccgatcga
     3481 cgcaaagccg ggcttggtgt aggtcgtcag cttgtcgaaa taggcgagct cgcgcagctt
     3541 cttttcgatc cggtcggtga cctggttctg catctcctcg gtcgtcgctc cgggccagat
     3601 ggcgctgaca ttgacgtttt tgatcgtgaa ggacgggtcc tcggcgcggc ccaattggag
     3661 ataggcgagc gcgccgccga ggccgagcgc gaggatcatg aagagcgtca gcgccggccg
     3721 gcggatcgcc cagagcgaga ggttgaacga acccatcaga gccccgcaat gtgacggacg
     3781 acgcgcacct tctgggttgg gtcgagctta tgggcgccga gcgccacgat cagcgcgccc
     3841 tccggcgccc cgccggcgat aaggacggat tcgctgtcgt aggcgacgac cttcacattg
     3901 gccggcgtga cggcgccgct cgcttcgtcg acgatgaaga ccatcggacc ctgcccttga
     3961 tcgagaatgg cgccgagcgg cgcgcgggcg actttttcgg aatcgagcgc gagcttgacg
     4021 gaagcgctca tgccgagcgc gacggaagcc ggcgcatcga caaggctgta gcgcgcttcg
     4081 tacgtacggg tcgtcggatc ggcgctcggc gagagctcgc gcaaaagcgc ctgcagcgtc
     4141 aggccgggca gcgcccagaa ttccgcttcg gcgggcgccg atttcgcccg ctcgaccagc
     4201 gcctcgggga tcgcaaccgc tgcttccttc tcgccgctat gggcgaggcg gacgaccggc
     4261 gcgccggcgg cgacgacctg gcccggctcg gccaaagccg ccgtgacgat cccgtcggcg
     4321 tccgcggtca gcgtcgcata agagagcgcg tcgccagcga gcgtcgcggc gcgctcggcc
     4381 ttgacgagat tggcgcgggc ctggtcggcg gcggccctga tcttgtcgag atcggcggcc
     4441 gccgaccagc cttgccggtg cagagtttcg acgcgggcca gatcggcgtc ctgttgctcg
     4501 cgcgcgcttt tcgcggcggc gagatcggcg tccgcttgct cctcttgcag cttgaggtcg
     4561 gaatcgtcga gctcggcgag cttctgcccc ttcttcacga ccgccccgga atcgaccagg
     4621 cggcgcgcga tcttgccggc gatgcggaag ccgagatcgc tttcgatgcg cgccttgacc
     4681 acgccgggca ggacgcggtc ggcgacgcgc ggggcgtaat ggatttgggc gacgaggacc
     4741 ggccggggcg gcgcctcgcg cttgacttcg ccatgcgcgt tgcagccggc gaggatgagg
     4801 gaaacgcaaa cgacaccgac cgaacgcagg cgcatgacac ggcgctccct aaggcgcgaa
     4861 cgagcagggg cggaattgcg gcagaggctg gcggctggcg agcgcgcgca aggcgaaggc
     4921 gacgagccct tgcgccaaaa gatcgatctc ctcgaccgtg cgcccctcgc cggcgcactt
     4981 ggcgatcatg gtcggatgga ggatcgaggc gcaggcgccg aaaaccaggt cggcgagctg
     5041 ggccggctcg ccggggccga attcgccggc cgcctggccc tcgccgataa gcttggcgac
     5101 cagccctttg caggattcca ggaaggcgtc gatggcgccc caattttcgt ccatcgccac
     5161 gacgaccatc tcatgcacgc gctcctggtt ggtcaggtga tcgcggtgat ggcggtgcag
     5221 cgtcatcagc agattgaaga cgcgctcgct cggggcgccc ggcgcgtcgg cggccgcggt
     5281 cgcggcttcg agcaggccgc cgagcagctt ggcgcaaagc gcctcgacga tggcgttctt
     5341 ggacgggaag aagcggtaga tgttggcgct cgacatgccg agcgcttggg cgatgtcgcc
     5401 gaccgtggtc ttgtaatagc cgatccgccg gaacagctcc tcggcggcga cgagaatgcg
     5461 ctcgcggctt tcgtcggggc tgatgcggag ggctaggatc atttgccgcg ctgacgattg
     5521 acgaatttca atttttatca gcgaacactc gtcaggtcaa gccgccgccg atccccagac
     5581 ggtcacagga tctatctcat ggaaaggcgg cgccgattcc gcccggcggg agtccgcgac
     5641 agatcaagcc ttggccgcgg cctattgcgg cggattgcgc ggcacgacga aaatctgatc
     5701 cggatagatc aggcgcggat tgcgaatctg cgaggcgttg gcttggtaga tgaccttgta
     5761 acgcggtccg tagccataga agcgcttgct caaattccaa agattgtcgc cgcgcacgac
     5821 tttcgtcgtc cggatttccg ggacgaccgg attggcgccg tcctgcttgg ctggcgcggc
     5881 ggccggcgcc ggcgttgcag agggcgtgct tgcggcgagg ctttgcgcct ggcgcggcgg
     5941 ctcggcgccc gccggccgcg gcgcggcttc ggccgcttgc ggcgacgcag gcgcggccgc
     6001 aatttcctcg gccggcttcg ccgaagcggt ctggtcggct ggctttacgg cgccgtccgc
     6061 cgccggcggc gccgccgctg cggcggcttc cccggtcggg cgcgcgggat agtcgaacgg
     6121 cacctcggcc cttgcgacta cggcgccatt ggcgcggtcg atctcatcgg cgcggatcgc
     6181 gtaaggcccc ggcgtcatgc cgcgctcgac ggtcagcgac cagcggccgt ccgcgccggc
     6241 cttcgcctcg gcgacgaaag cgttgttgag gtagagcctg accagggcgt tcggttcggc
     6301 ggcgcctttc gcctcgaacc ggccgacgcc gttggtctcg acggagagga tcgcgacgag
     6361 cggcgcgctc gcggcctgct tgctcggctc cgcgcccagc gcggcgactt tcggcggctc
     6421 cggcgcgggc gtcgccttcg gagcggcagg cgcgcccgct ttcggagcag ccgcttcggc
     6481 caggtgctga ggctcgaccg ggttctgcgc gctcgccgcc tgtttttccg gcgattgcgg
     6541 ggcgttttgc cgggcggact cggccgtggc cggcacctgg atgccgatcg catcggaaag
     6601 aacctccgct ccgcccgacc gcgccgccaa ttgcaggcga tgatccccgg ccgggaaggg
     6661 cggcggcagg atggcgaact ggcccatttc gtcggcgtcg acttgcgcga cgacgcggcc
     6721 gtcggagcgc aattgcaccg cggcgcgcgg cgaggcgcgc ccggcgatca ccgcatcgcc
     6781 gctcggctca acccgcacca cgtcgaaggc gggccgctgc gcctcggctt tggccggcgg
     6841 cgcggcttgg gtggcgtggg tcgtcggggt tgacggcgtc gcctgctgcg ggccggccgc
     6901 cgccgggctc gacggagcgg gagccgcagc cggcgccgcg gaagccgcag ctggcggcgg
     6961 cggactggcg gaaggcggcg cggcggcgct cgcggcacgc tcgccgggcg ccggctgaga
     7021 accccccggc gaggcggcgg cttcgccctt tggcggcggc gcggcggacg gggcggcgac
     7081 cggcggcgcc gaactcgccg caggctcggc cggcttggcc ggggcttcgg ccgccgcgct
     7141 ctccggcgcc ttgagcggag cggctggcgc ggcggcctcc cggtcgccgc tttcggcagc
     7201 cttctgcgac aaagcgggcg gcgccgcggc ggcgcccggc cagccgatat cgtaaaattt
     7261 caggatcgcc ggcgtcaccg ccgcaaccgc tacgacgccg agccccgccg cccagtagat
     7321 cgcggttttc gacatcatgg aaaagggtcc tcgaacggct taaaaaagcc tctatatcaa
     7381 taatgttact cccgccatgg gtcccggcaa gccggggcta tcgatctgtt gacagcgggt
     7441 ttggcccagc ctaaagcatc gcccgacagc gcggcgggtt cagccgcgct ttttcgcgtg
     7501 tgcgcggctc gatccgcggc gcgcgaagcc aatagcaggt ccccagctgc aaagaagccg
     7561 gccgcgccaa gcgcagagcc gggctgaaca cggagaaaaa acgatgttcg cagtgatcaa
     7621 gaccggcggc aaacagtaca aggtcgccgc cgaggatgag attaccgtga tgaccctcgc
     7681 cggcgagccc ggcgagactg taacgttcgg cgaggttctg gcgctgttcg acggcgatac
     7741 gaaccatgtc ggcgcgccgc atatcgaagg cgccagcgtc gccgggacga tcgtc
//
LOCUS       DATGJW010000261         3632 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_463621, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000261 DATGJW010000000
VERSION     DATGJW010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3632)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3632)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3632
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_463621"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VKU03_08210"
     CDS             complement(<1..870)
                     /locus_tag="VKU03_08210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015670285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ71285.1"
                     /translation="MRAPAPRAPRDWIEWPFFEPRHRDFAERLDRFVASSAMAEIDHA
                     DADRACRKLARALGEAGLLAAAVAPGDGDAAAIDSRAACLARETLAWSDGLADFAFAM
                     QGLGAGAIGLAGSPEMKAAVLPKARAGEWLAAFALSEKEAGSDVAAMACAARLEGNQY
                     VIDGEKTWISNGGIADVYTLFARSGEAPGARGISAFVVFPDDPGFSIAERIEVIAPHP
                     LATLRFDNCRIPKSRLLGAPGGGFKLAMRTLDIFRASVAAAALGFARRALDEALAHAR
                     SRRMFGGALADLQL"
     gene            complement(875..1726)
                     /locus_tag="VKU03_08215"
     CDS             complement(875..1726)
                     /locus_tag="VKU03_08215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase family protein"
                     /protein_id="HLJ71286.1"
                     /translation="MSETPSAMQASRQPFKGHQARHFEFSTSDDGRVATVRLNRPERK
                     NPLTFESYAELRDLFRALVYASDVRAVVLAGSGGNFCSGGDVFEIIEPLTRMAMPDLV
                     AFTRMTGDLVKAMRRCPQQIVAAVDGVCAGAGAILAMASDIRFATPEAKTAFLFTRVG
                     LAGADMGACGILPRLIGQGRAAELLYTGRTMSAAEGAAWGFYNALHPQAALEREAAAF
                     ARALADGPWFAHGMTKTMMNQEWAMGLYEMIESEAQAQAICMATGDFRRAFEAFAAKR
                     KPRFEGN"
     gene            complement(1723..2511)
                     /locus_tag="VKU03_08220"
     CDS             complement(1723..2511)
                     /locus_tag="VKU03_08220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71287.1"
                     /translation="MTAGPLAERHALVTGAGGGIGAAVARAFAAAGARVSLAGRRREP
                     LAAVAAALAPGAALILDGFDVTDAAAIARGADAARAAFGAVSILVNNAGEAPSAPFEK
                     TDLALWRHVLAVDLTGAYQVTREILPDLKRHGPGARILNIASTAGLTGYAYVSAYCAA
                     KHGLVGLTRALALELARTGITVNAICPGFTETPLIERAVETIAAKTGRDRDAARAELA
                     KANPQGRLIRPEEVADAALWLASPGAGSINGQAIAIAGGEVMAG"
     gene            complement(2508..>3632)
                     /locus_tag="VKU03_08225"
     CDS             complement(2508..>3632)
                     /locus_tag="VKU03_08225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270780.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HLJ71288.1"
                     /translation="RMSTPLLFQPITFRSVTARNRIVVAPMCQYSAEDGLGDDWHVQH
                     LGARAMGGAGLVFAEMTCVSPNARITPGCLGLWNDAQQEGWRRLVDLIHTVGHAKAGV
                     QLGHAGRKGSTKVAWEGIDQPLESGGWQLISASAIPYLPHSPVPRAMTRADMDRVRDD
                     FIAATRRAAAAGADWLELHCAHGYLLSSFLSPLTNLRDDEYGGGLENRARYPLEVFRA
                     VRAAWPAEKPISVRLSCHDWTPGGNTPDDALVFARMFKEAGADLIDCSSGQVSKAERP
                     VYGRLFQTPFSDQIRNEGGVATIAVGAISEADHANSIVAAGRADLCAIARPHLADPAW
                     TLHEAARIGFNDVAWPKQYLAAKGQYETNLARAAALAAAK"
BASE COUNT          558 a         1258 c         1292 g          524 t
ORIGIN      
        1 gagctgcagg tcggccagcg cgcccccgaa catgcggcgg gagcgggcat gggcgagcgc
       61 ctcgtcgagc gcgcggcggg cgaagccgag cgccgcggcg gcgaccgagg cgcggaaaat
      121 atcgagcgtt cgcatcgcga gcttgaagcc gccgccgggc gcgccaagca gccggctttt
      181 cgggatgcgg cagttgtcga agcgcaatgt cgccagcgga tgcggcgcga tcacctcgat
      241 tcgctcggcg atcgagaagc cgggatcgtc ggggaagacg acgaacgcgc tgatcccgcg
      301 cgcgcccggc gcctcgccgc tacgcgcgaa cagcgtatag acatcggcga tcccgccgtt
      361 cgaaatccag gtcttctcgc cgtcaatgac gtactgattg ccttcgagtc gcgcggcgca
      421 ggccatcgcg gcgacatcgg agcccgcttc tttctccgac agcgcgaagg cggcgagcca
      481 ctcgccggcg cgcgccttcg gcaggacggc cgccttcatt tcgggcgagc cggcaaggcc
      541 gatcgcgccg gcgccaagcc cttgcatcgc gaaggcgaag tcggcgaggc cgtcgctcca
      601 ggcgagggtt tcgcgggcga gacaggccgc gcgcgaatcg atcgccgcgg cgtcgccgtc
      661 acccggcgcg acggcggccg cgagcaggcc cgcttcgccg agcgcccgcg ccaatttacg
      721 gcaggcgcgg tccgcatcgg cgtggtcgat ctccgccatc gcggaagaag cgacgaagcg
      781 gtcgagccgt tcggcgaagt cgcgatggcg cggctcgaaa aacggccact cgatccagtc
      841 gcgcggcgcg cgcggagcgg gagcgcgcac gacctcaatt cccctcgaag cgcggcttgc
      901 gcttggcggc gaaggcctcg aaggcgcggc ggaaatcgcc ggtcgccatg caaatcgcct
      961 gcgcctgcgc ctccgattcg atcatctcgt acagccccat cgcccattcc tggttcatca
     1021 tcgtcttggt cattccgtgg gcgaaccatg gcccgtcggc gagcgcgcgc gcaaaagccg
     1081 ccgcctcgcg ttcgagagcg gcttgcggat ggagcgcgtt gtagaaaccc caggcggccc
     1141 cttcggcggc gctcatcgtg cggccggtat agagcagctc ggcggcgcgc ccctggccga
     1201 tcaggcgcgg caggatcccg caagcgccca tatcggcgcc ggcaaggccg acgcgggtga
     1261 acaggaaagc ggtcttcgcc tcgggcgtcg cgaagcgtat gtccgacgcc atagccagga
     1321 tcgcgccggc gccggcgcag acgccgtcga ccgccgcgac gatctgctgc gggcagcgcc
     1381 gcatcgcctt gacgagatcg ccggtcatcc gcgtgaaggc gacgagatcg ggcatggcca
     1441 tgcgcgtcaa tggctcgatg atctcgaaca cgtcgccgcc cgagcagaaa ttgccgcccg
     1501 atccggcgag cacgacggcg cggacgtcgg acgcatagac aagagcgcgg aagaggtcgc
     1561 gcagctcggc atagctctcg aaggtcagcg gattcttccg ctccggccgg ttgagccgca
     1621 ccgtcgcgac gcgcccgtcg tcgctggtcg agaattcgaa atggcgcgcc tgatgccctt
     1681 tgaacggctg ccgcgacgcc tgcatcgccg aaggggtttc gctcatcccg ccatgacctc
     1741 cccgccggcg atcgcgatcg cctgtccgtt gatcgatccg gcgccgggcg atgcgagcca
     1801 aagcgccgcg tcggcgacct cttccggcct gatcagccgg ccttgcggat tcgccttggc
     1861 gagctccgcc cgcgccgcgt cccggtcgcg gccggtcttg gccgcgatcg tttcgaccgc
     1921 ccgctcgatc agcggcgtct cggtgaagcc tgggcagatc gcgttgaccg tgattccggt
     1981 ccgcgccagt tcgagcgcca gcgcgcgcgt cagcccgaca aggccgtgct tggccgcgca
     2041 ataggccgag acataggcgt agccggtcag ccctgcggtc gaggcgatgt tgagaatgcg
     2101 cgcgccaggc ccgtggcgct tcaaatcggg caggatttcg cgcgtgacct ggtacgcgcc
     2161 ggtcaggtcg acggcgagga catggcgcca cagcgcgagg tcggttttct cgaaaggcgc
     2221 gctcggcgct tcgccggcat tgttgacgag gatcgagacc gcgccgaagg cggcgcgcgc
     2281 cgcgtccgcg ccgcgcgcga tcgcggcggc gtcggtgacg tcgaatccgt cgagaattaa
     2341 ggccgcgccc ggcgcgagcg ccgccgcgac cgcagcgagc ggctccctgc ggcgtccggc
     2401 gagactgacg cgcgcgccgg ccgcggcgaa agcgcgggcg accgccgcgc cgatgccgcc
     2461 gcccgccccg gtgacgagcg cgtgccgttc ggcgagcggg cccgcggtca tttcgctgcc
     2521 gccaaggcgg cggcgcgcgc caaattggtc tcgtattgcc ccttggccgc gagatattgc
     2581 ttcggccaag ccacgtcgtt gaagccgatg cgcgccgcct cgtgcagcgt ccaggccggg
     2641 tcggcgagat gcgggcgggc gatcgcgcag agatcggcgc gacccgccgc gacgatggaa
     2701 ttggcgtggt cggcctccga gatcgcgccg acggcgatcg tcgcaacgcc gccctcgttg
     2761 cggatctggt cggagaacgg cgtctggaac agccggccat agaccggccg ctccgccttc
     2821 gaaacctgcc cggacgagca atcgatcaga tcggcgccgg cttccttgaa catccgcgcg
     2881 aagaccagcg cgtcgtccgg cgtgttgccg cccggcgtcc agtcgtgaca ggagagccgc
     2941 accgagatcg gcttctccgc tggccatgcg gcgcggacgg cgcggaacac ttcgagcgga
     3001 tagcgcgcgc gattttcgag gccgccgccg tattcgtcgt cgcgcagatt ggtgagcggc
     3061 gacaggaagc tcgacagaag atagccatgc gcgcaatgca gttcgagcca gtcggccccg
     3121 gccgccgccg cgcgcctcgt tgcggcgatg aagtcgtcgc ggacccggtc catgtcggcg
     3181 cgcgtcatcg ccctcggaac cgggctatgc ggcaggtagg gaatggcgga agctgaaatc
     3241 aactgccagc cgccgctctc cagcggctgg tcgatgccct cccaggcgac cttggtcgag
     3301 cctttgcggc cggcatggcc gagttggacg cctgctttgg cgtgaccgac cgtgtggatg
     3361 agatcgacga gccgccgcca tccctcctgc tgcgcgtcgt tccacaggcc gaggcagccc
     3421 ggcgtgatgc gcgcgttagg cgagacgcag gtcatctcgg caaaaacgag acccgccccg
     3481 cccatggcgc gcgcgccaag gtgttgtacg tgccagtcat cgccgagacc gtcctcggcg
     3541 ctgtactggc acatgggtgc gacaacgatg cggttgcgcg cggtgaccga gcggaaggtg
     3601 atcggctgga acagcagcgg cgttgacatg cg
//
LOCUS       DATGJW010000262         4135 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1087065, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000262 DATGJW010000000
VERSION     DATGJW010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4135)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4135)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4135
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087065"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..462)
                     /locus_tag="VKU03_08230"
     CDS             complement(<1..462)
                     /locus_tag="VKU03_08230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLJ71289.1"
                     /translation="MSDFHRVRRLPPYVFEQVNKLKAKARAAGADIIDLGMGNPDLPA
                     PKHVLDKLVETVGKPRTDRYSASKGIPGLRRAQAGYYARRFGVKLNPETQVVATLGSK
                     EGFANMAQAITAPGDVVLVPNPSYPIHAFGFLMAGGVIRSVPADPTPDFFVA"
     gene            complement(565..1026)
                     /locus_tag="VKU03_08235"
     CDS             complement(565..1026)
                     /locus_tag="VKU03_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71290.1"
                     /translation="MFARRRDRRAAGWALALAAALAVAAPAQAGEDAPASGAPQAQSD
                     DGFLKSVLKDAHMTTDDLGHPQDFVVKSRPKAPADYVPVFRKPEEHKTKVLSPDQVKA
                     MQAELDAAGARNAQIRDAFPPSRRAYRQEEREKAAKAAAKRAKKPAAASVQ"
     gene            1343..3229
                     /gene="phaC"
                     /locus_tag="VKU03_08240"
     CDS             1343..3229
                     /gene="phaC"
                     /locus_tag="VKU03_08240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0042619 - poly-hydroxybutyrate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I poly(R)-hydroxyalkanoic acid synthase"
                     /protein_id="HLJ71291.1"
                     /translation="MSTPLPTPAQPAKGAPQPEAAKPAASLPPSAATDKAPVPAALPN
                     FEALSRNLARFIEESGKLASAYLAPRGGDAQSPLANMVADAMATFGKIAEGYFADPQR
                     AFHAQSALSKQFVELWAATLHRLQGEKAEPVAPPDPADKRFSDPEWRDNPYYDFLRQA
                     YTIATRWAGDVIQTAEGVDPHTRDKAQFYMRQFAAALSPTNFLPTNPELMRTTFAESG
                     ENLVRGLQMMNEDIAAGKGQLRIRQTDARKFELGKNMAATPGKVVFRNALMELIQYAP
                     STETVFKRPLLIVPPWINKFYVLDLNPEKSFIRWAVAQGLTVFVVSWVNPDERHADKD
                     FEAYMREGIFAALDAIEQATGERQVSAIGYCVGGTLLSVALAYMAAKGDDRIAGATLF
                     AAQVDFRDPGDLKVFVDELQLKAIAEQMAQKGYLEGAQMANAFNMLRPNDLIWSYYVN
                     NYLKGKEPPAFDLLVWNSDSTRMPAANHMFYLRNCYLENNLTQGRMEIAGVKLDLSRV
                     TIPVYSLAAREDHIAPAKSVFVGARFFGGPVRYVLAGSGHIAGVVNPADKAKYQYWTG
                     GPPQGEFEDWLAKAKETPGSWWRDWIQWIAEQAPEKVPARTPGGGKLQPLCDAPGEYV
                     RARV"
     gene            3523..>4135
                     /locus_tag="VKU03_08245"
     CDS             3523..>4135
                     /locus_tag="VKU03_08245"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ71292.1"
                     /translation="MPADDGAKSVAPASPAAVRQWLEALGLGQYAPAFAENDIDFTVL
                     AQLADADLRELGVHSLGHRKRLLSAIAARSAGLAETAPAGVSVPAEGERRQVTILFAD
                     LSGFTALSQALDPEQVRELVGRFTALVDGVIVGYGVTVDKHIGDAVMALFGAPKAHDD
                     DPLRAARAALDIHEALGQWSVSAGRKLQAHIGVASGEVVADAID"
BASE COUNT          706 a         1354 c         1355 g          720 t
ORIGIN      
        1 cgcgacgaag aaatccggcg tcggatcggc cgggaccgag cggataacgc cgccggccat
       61 cagaaagccg aaggcgtgga tcgggtacga cggattgggc acgagcacga cgtcgcccgg
      121 cgcggtgatc gcctgggcca tgttggcgaa gccctctttc gagccgagcg tggcgacgac
      181 ctgcgtttcc ggattcagct tcacgccgaa gcggcgggca tagtagccgg cctgggcgcg
      241 gcgcaggccg ggaatgccct tggaggccga atagcggtcg gtgcgcggct tgccgaccgt
      301 ctcgaccaat ttatcgagca cgtgcttggg cgccgggaga tcgggattgc ccatgccgag
      361 atcgataatg tccgccccag cggcgcgggc cttggccttg agcttgttga cctgctcgaa
      421 gacgtaaggc ggcaggcgcc gcacgcggtg aaaatcgctc atcgaggctt tgtccccttt
      481 ccgccggcgg attcggagct ttgccggccg gcttgtttag cgccgcagcg tcagccgcgg
      541 caaattccga cttgtttcat tctatcattg tactgaggcg gccgccggct ttttcgcgcg
      601 tttggcggcg gccttggccg ccttctcgcg ctcttcctgc cgataggcgc ggcgggaagg
      661 cgggaaggcg tcgcggatct gggcgttgcg cgcgccggcg gcgtcgagct cggcctgcat
      721 cgccttgacc tgatccgggc tcagcacctt ggtcttgtgc tcttcgggct tgcggaagac
      781 cggcacgtaa tcggccggcg ccttgggccg cgacttgacg acgaaatcct gcggatggcc
      841 gagatcatcc gtggtcatat gcgcgtcctt caggacgctt ttgagaaagc cgtcgtcgct
      901 ctgcgcctgc ggcgcgccgg aagcgggcgc gtcctcgccc gcctgggccg gcgcggcgac
      961 ggcgagggcg gcggcgagag ccagcgccca gcctgccgcg cgccgatcgc ggcggcgcgc
     1021 aaacattgcg taaactcttt gaagcacgcg cattcggcgc cggtcgcctt tcgatactcg
     1081 ttttgccgtt atcgtcctat tatgtccgaa acgggacccg cgccaaatcc ggctcgggtc
     1141 cgaaagatcg aggcgcgtta tgtctaccga tccggtcgag cggcgccctg cgcggaacgg
     1201 cccggcagcg gccgattccg cgaaagcgga gaccgccaag ccggccgccg cgggcaagcc
     1261 cgcgccgcgg gcgaaaaaac cgcgcgccag cgccaaaaac aatggcgcgc cggccgattt
     1321 cgctcgccaa gccgaggcgg ttttgtccac gcctctcccg acgcccgccc agccggcgaa
     1381 gggcgcgcct cagccggaag cggccaagcc ggcggcttcg ctcccgccga gcgccgcgac
     1441 cgataaggcg cccgtcccgg cggcgctccc gaatttcgag gccctgtcgc gcaatctcgc
     1501 ccggttcatc gaggaaagcg gcaagctggc ttccgcctat cttgcgccgc gcggcggcga
     1561 cgcccaatcg cctctggcca atatggtcgc cgacgcgatg gcgacattcg gcaagatcgc
     1621 agagggctat ttcgccgatc cgcaacgcgc attccacgcc caaagcgcgc tgtccaagca
     1681 attcgtcgag ctctgggcgg cgactttgca tcgcctgcaa ggcgaaaagg ccgagccggt
     1741 cgcgccgccc gacccggccg acaagcgctt ttccgacccg gaatggcggg acaatcccta
     1801 ttacgacttc cttcgccagg cctatacgat cgcgactcgc tgggccggcg acgtgatcca
     1861 gaccgccgaa ggcgtcgacc cgcatacccg cgacaaggcg caattctata tgcgccagtt
     1921 cgccgccgct ttgtcgccga ccaatttcct gccgaccaat cccgagctga tgcgcacgac
     1981 cttcgccgag agcggcgaaa atctcgtccg cggcctgcaa atgatgaatg aggacatcgc
     2041 cgccgggaaa ggccagttgc gcatccgcca gaccgacgcg cgcaaattcg agctcggcaa
     2101 gaacatggcc gcgacgccgg gcaaggtcgt gttccgcaac gcgctgatgg agctcatcca
     2161 atacgcgccg tcgaccgaga ccgtgttcaa gcggccgctt ctgatcgtcc cgccctggat
     2221 caacaagttc tacgtgctcg acctcaatcc ggaaaaatcc ttcatccgct gggctgtggc
     2281 gcagggcctg accgtcttcg tcgtctcctg ggtcaacccc gacgagcggc acgccgacaa
     2341 ggatttcgag gcctatatgc gcgaggggat tttcgccgcc ctcgacgcga tcgagcaggc
     2401 gaccggagag cgccaggtct cggcgatcgg ctattgcgtc ggcggcacgc ttctgtctgt
     2461 tgcgctcgcc tatatggcgg cgaaaggcga cgaccggatc gccggcgcga cgctgttcgc
     2521 cgcccaggtc gatttccgcg atcccggcga cctcaaggtg tttgtcgacg agttgcagct
     2581 caaggccatc gccgagcaga tggctcagaa aggctatctc gaaggcgcgc aaatggccaa
     2641 cgccttcaat atgttgcggc cgaacgatct gatctggtct tattacgtaa acaattacct
     2701 gaagggcaag gagccgccgg ctttcgacct cctggtctgg aattcagatt cgacccgaat
     2761 gccggccgcc aatcatatgt tctacctgcg caattgttat ctcgaaaaca atctgactca
     2821 gggccggatg gagatcgccg gcgtcaagct cgacctctct agagtgacca ttccggtcta
     2881 tagcctggcg gcgcgagagg accatatcgc gccggccaaa tcggtctttg tcggcgcgcg
     2941 gtttttcggc ggcccggtgc gctatgtgct cgccggctcc ggacacatcg ccggcgtcgt
     3001 taacccggcc gacaaggcga aataccagta ttggaccggc ggaccgccgc agggcgagtt
     3061 cgaggattgg ctcgccaaag ccaaggagac gccgggaagc tggtggcgcg actggatcca
     3121 gtggatcgcc gagcaggcgc ctgagaaagt ccctgcgcgc acgcccggcg gcggcaaact
     3181 ccagccgctc tgcgacgcgc caggcgaata tgtgcgggcg agggtctagc ccggcgccga
     3241 gcgttgcgcg ccgagccgac aaattccctc cgtcacggcg tcgcctccag gcggacggaa
     3301 gatgtgatcg ctctcacaca ttgtgagatg ggaatttcaa acgaagcagc ttgggatcgt
     3361 cggctaaagt actgagggaa gtcattcctg cggccttttc ggttgcggcg cggctgacat
     3421 tgcaacccag tttctggagt cggaatctca cttgaatcgc ggccgctgac atgcaggagt
     3481 ctgtcggctc gtgagttcct gcgctcgttt tgggggccaa acttgccggc agacgatggg
     3541 gccaagagcg ttgcgcctgc gagcccagcc gcggttcggc aatggctgga ggcgctcggg
     3601 ctcggccaat acgcgccggc cttcgccgaa aacgacatcg actttacggt gctggcgcag
     3661 ctcgccgacg ccgatttgag ggagctcggc gtgcattcgc tcggccaccg caagcggctg
     3721 ctgagcgcga tcgccgcaag gagcgcgggg ctggccgaaa cggcgccggc gggcgtttcc
     3781 gttcccgccg agggcgagcg ccggcaagtg acgatcctct tcgccgatct ctccggtttc
     3841 accgccttgt cgcaggcgct cgatccggag caggttcgcg agctcgtcgg gcgcttcacc
     3901 gcgctcgtcg atggcgtcat cgtcggctat ggcgtgacgg tcgataagca tatcggcgat
     3961 gcggtaatgg cgcttttcgg cgcgcccaaa gcgcatgacg acgatccctt gcgcgcggcg
     4021 cgcgcggctt tggacattca cgaagccctc ggccaatgga gcgtaagcgc cggccgcaag
     4081 ctacaggctc atatcggcgt cgccagcggc gaggtggtgg ccgacgcgat cgacc
//
LOCUS       DATGJW010000263         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1187863, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000263 DATGJW010000000
VERSION     DATGJW010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1187863"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1132
                     /locus_tag="VKU03_08250"
     CDS             <1..1132
                     /locus_tag="VKU03_08250"
                     /inference="COORDINATES: protein motif:HMM:NF013781.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLJ71293.1"
                     /translation="LGEARALIAFRLAADADELATISKFRALRIAWSRVEEACGLEPR
                     AAHVEAESAWRMMTARDVYVNVMRGATAAFAAGLGGADSVSVLPHTLAAGLPDNLARR
                     LARNAQLILLRESNLGFVADPAAGAGAFEALTKALCEKAWGFFQELERQGGLPAALAN
                     GAFQRQVAVSAATLARNVARLKSPITGVSAHASLDEPPVETAAGAPERDGFAPAGGAL
                     GPIRLAEPFERLRDLSDAFLLRTGARPNVYLAALGPEPKHRRRVAFTREWLAAGGFAA
                     VYDGEAVTTEQAVGRLRRSGAPLVCLCGTDEVYAEQAEILAKAFKAAGAKGVALAGAP
                     REFERAWHAAGVDEFIFAGADAVAALEALYRHIGVETKVRAR"
     gene            1137..>2527
                     /locus_tag="VKU03_08255"
     CDS             1137..>2527
                     /locus_tag="VKU03_08255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLJ71294.1"
                     /translation="MTQIPDFSNLPFAAPRRPIVASGSQTWRTPEGIDVKSLYGPADR
                     AGLDLAHDWPGLPPFLRGPYPTMYLTQPWTVRQYAGFSTAEESNAFYRANLAGGQKGL
                     SVAFDLATHRGYDSDHPRVAGDVGMAGVAIDSIYDMRTLFSGIPLDSMSVSMTMNGAV
                     LPVMALFIVAAEEQGAPLAALSGTIQNDILKEFMVRNTYIYPPAASMRIVADIFAFTA
                     ANMPKFNSISISGYHMQEAGATQDLELAYTLADGLEYVRAGVGAGMSIDTFAPRLSFF
                     WAVGMNFFMEVAKLRAARKLWAGIVKGFGPKSDKSLALRAHCQTSGWSLTAQDPLNNI
                     VRTEIEAMAAAQGHTQSLHTNALDEALALPTPFSARIARNTQLFLIEETGTTRVVDPW
                     GGSYYLERLTAELAARAQAHIEEVERLGGMAKAIGSGLPKRRIEEAAARTQARIDSGR
                     QAVIGVNIHPPTI"
BASE COUNT          384 a          858 c          853 g          432 t
ORIGIN      
        1 gcttggcgag gctcgggcgc tgatcgcgtt ccgcctcgcc gcggacgcgg atgaattggc
       61 gacaatctcg aaattccggg cgctgcgcat cgcctggtcc cgcgtcgaag aggcctgcgg
      121 acttgagcct cgcgcggcgc atgttgaggc cgagagcgcc tggcggatga tgacggcgcg
      181 tgatgtgtat gtgaacgtca tgcgcggcgc gacggcggcg ttcgcggccg gcctcggcgg
      241 cgccgacagc gtgagcgtcc tgccgcatac tctggccgcc ggccttcctg acaacctcgc
      301 gcggcgcctc gcgcgcaacg cccagctcat tctcttgcgc gaatccaatc tcggatttgt
      361 cgccgacccc gccgccggcg ccggcgcgtt cgaggcgctg accaaggccc tttgcgagaa
      421 ggcctggggt ttttttcagg agctcgaacg gcagggcggc ctgccggcgg cgctcgcgaa
      481 tggcgcgttc caacgccagg tcgccgtgag cgccgcgact ctcgcccgaa atgtcgcccg
      541 gctgaaatcg ccgatcacgg gggttagtgc gcacgccagt ctcgacgagc cgccggtcga
      601 aacggccgcc ggcgcgcccg agcgggacgg gtttgcgccg gccggcggcg cgctggggcc
      661 tattcggctc gccgagccgt tcgagcggtt gcgcgatctc tccgacgcct ttcttctgcg
      721 gaccggcgcg cggccgaacg tctatctcgc cgcactgggg ccggaaccca agcatcggcg
      781 ccgggtcgcg ttcacgcgtg aatggctggc ggcgggcggt ttcgcagcgg tctacgatgg
      841 cgaggcggtc acgaccgaac aggcggtcgg acgcctgcgg aggagcggcg cgccgctcgt
      901 ctgcctgtgt gggaccgacg aggtctatgc ggaacaggcg gaaatccttg cgaaagcgtt
      961 caaggctgcg ggcgcaaaag gcgtcgcgct cgcgggcgcc ccgcgcgaat tcgagcgggc
     1021 gtggcacgcc gcgggcgtcg acgaatttat ttttgccggc gcggacgccg tcgcggcgct
     1081 ggaggcgctc tatcgccata ttggagtcga aaccaaggtc cgggcgcggt gacggcgtga
     1141 cgcaaatccc cgatttctcg aacctgcctt ttgcggcccc ccgccgcccg atcgtcgcga
     1201 gcggcagcca aacctggagg acgcccgagg gcatcgacgt caagtcgctc tacgggcccg
     1261 ccgatcgcgc cggactcgat cttgcccacg actggcccgg gctcccgccc tttttgcgcg
     1321 ggccgtatcc gacgatgtac ctcacgcagc cgtggacggt gcggcaatat gccggcttct
     1381 cgaccgccga ggagtcgaac gctttctatc gcgccaatct cgccgggggc cagaaaggcc
     1441 tgtcggtcgc tttcgacctc gccacccatc gcggctacga ctcggaccat ccgcgggttg
     1501 ccggcgatgt cggcatggcg ggcgtcgcca tcgattccat ttatgacatg cgcacccttt
     1561 tctccggcat cccgctcgat tcgatgagcg tgtcgatgac catgaacggc gccgtgctgc
     1621 cggtgatggc gctgttcatt gtcgccgccg aggagcaggg cgcgccgctt gccgcgctct
     1681 cagggacgat tcagaacgat attctcaaag agttcatggt ccgcaacacc tacatttatc
     1741 cgcccgccgc ctcgatgcgg atcgtcgccg acattttcgc cttcaccgcg gccaacatgc
     1801 cgaaattcaa ctcgatctcc atctccggct atcacatgca ggaggcgggc gcgacgcagg
     1861 atctcgagct cgcctacacg ctcgccgacg gcctcgaata tgtgcgcgcc ggggtgggcg
     1921 ccggaatgag catcgatacg tttgccccgc ggctctcgtt cttctgggcg gtgggcatga
     1981 attttttcat ggaagtcgcg aagctccggg ccgcgcgcaa gttgtgggcg ggcatcgtca
     2041 agggcttcgg gcctaagtcg gacaagtcgt tggctttgcg cgcccattgc cagacttccg
     2101 gctggtcctt gaccgcgcag gatcctctca acaacatcgt gcgcaccgag atcgaggcga
     2161 tggccgcggc gcaagggcat acgcagtcgc tgcacaccaa tgcgctcgac gaggcgctgg
     2221 cgctgccgac ccccttctcg gcccgcatcg cccgcaacac ccagcttttc ctgatcgagg
     2281 agacgggaac cacgcgggtc gtcgacccct ggggcggctc ctattacctc gagcgtctca
     2341 ccgcggaact ggccgcgcgg gcgcaggccc acatcgagga ggtcgagcgc ctcggcggaa
     2401 tggcgaaggc gatcgggagc ggcctgccta agcgccgcat cgaagaggcg gcggcgcgca
     2461 ctcaggcgcg gatcgattcc ggccgccagg cggtgatcgg cgtcaacatc catccgccga
     2521 ccatcga
//
LOCUS       DATGJW010000264         8895 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1087371, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000264 DATGJW010000000
VERSION     DATGJW010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8895)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8895)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8895
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087371"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..261
                     /locus_tag="VKU03_08260"
     CDS             <1..261
                     /locus_tag="VKU03_08260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403654.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipid-A-disaccharide synthase"
                     /protein_id="HLJ71295.1"
                     /translation="YLVRVPSILLPNLILGERAIPEILQGECKPERLAGLLGALIRDS
                     AERQAQLAALARLDKRMRLDTGETPSARAARLTLETVEAAVR"
     gene            364..2193
                     /gene="treZ"
                     /locus_tag="VKU03_08265"
     CDS             364..2193
                     /gene="treZ"
                     /locus_tag="VKU03_08265"
                     /inference="COORDINATES: protein motif:HMM:TIGR02402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose trehalohydrolase"
                     /protein_id="HLJ71296.1"
                     /translation="MSLLHPDDVAKLESARRSPPESALGAPFGATAAPEGVFFRLWAP
                     ACDNVSLLLESETEPRPMQPRPGGFFEAFVPGFGAGTLYRYLLPNGMKVPDPASRFQP
                     GDVDGPSEVIDPAAYAWSQSWAGRPWDDIVLYELHVGAFTPEGTFRAAAEKLDHLAQL
                     GVTAIEIMPVADFQGRWNWGYDGALLYAPDSSYGRPEDFKSFVEAAHARGVAVLLDVV
                     YNHFGPAGNYLRLYAPSFFTDRHKTPWGDAINYDGEDSRPVRDFVIANAQYWIDEFHL
                     DGLRLDAVHAIADDSPKHILDELAERLRATAKRPLHLIVEDENNEPRRLRRHGRAPIL
                     FTAQWNDDVHHVLHVAATEERAGYYEGYLKDGELLGKALAEGFAYQGQVMPYRGRARG
                     APSAFLPPGAFVAFIQNHDQVGNRAFGERLNMIARPEAMRALAAVYLLLPQAPMIFMG
                     EEWGAEQPFLFFCDFSGDLAEAVREGRRAEFARFPEFADPAARDKIPDPVAESAFLAS
                     KLDWRRMNQNRLAHYRALLKTRRRCIAPLLPHIQSGGRAVIFGPEAVRVRWQARERIL
                     ILDANLCGRRSDFPPARGRIFWSEGETAGALGPWSVRWSVEAE"
     gene            2190..4919
                     /gene="treY"
                     /locus_tag="VKU03_08270"
     CDS             2190..4919
                     /gene="treY"
                     /locus_tag="VKU03_08270"
                     /EC_number="5.4.99.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047470 - (1,4)-alpha-D-glucan
                     1-alpha-D-glucosylmutase activity [Evidence IEA];
                     GO_process: GO:0005992 - trehalose biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malto-oligosyltrehalose synthase"
                     /protein_id="HLJ71297.1"
                     /translation="MTAPRATYRLQFHAGFTFRRAAAIAPYLGRLGVSHVYASPYLKA
                     RPGSAHGYDITDHNALNPEIGSEDDHAAMIEAFKREGLGLILDFVPNHMGVGGADNPF
                     WLDVLEWGQDSNYAGWFDIDWASDDPYLGDKLLAPFLGDQYGVELKAGKLALKYDAEE
                     GAFAIWAYDYHKLPVCPLHYGLIVGAETPGLERLGDLFGDLRQWRPQIAERAKTLRAE
                     LAAHIRADEQARQALTARIAAFNADWRALDGLIAKQNWRIAYYGAASDEINYRRFFNI
                     NELAGLRVELPALFRRMHARVLAMLEDGAIDGLRLDHVDGLFDPKAYFEALRKSAARP
                     FYLVVEKILAPHEQLREEWPVDGTTGYDYANLSLGVLINGAAEESFSQTYRDFAGAAA
                     PFDEIARACKLRIMDNEMASELNALSRAGVRLAHQNPLTTDFSRNILKRAIRLIVASL
                     PVYRTYIDMSGAPSEADLRDLDWAVAQARREDPDIHPSVFAFLRAALSGALVAAPNSG
                     YSRTACLRFAMKLQQYSGPVMAKGVEDTAFYRYNRHIALNEVGGAPERFGVSINAFHK
                     ANAVRAKNWPRAMLATTTHDTKRGEDGRARLAVLSDIPEEWRREVNAWSRIIRARRGD
                     VERQAPPDRNDEYMFYQLLIGSWPAELMLELDEGALEKYRQRVLGALEKSLREAKLHT
                     SWTAPNPDYEEAMRSFALDALDLLRSGFLTNFLPFVRRIARLGVDNSLAQTAMKLTLP
                     GVPDFYQGSELWNLSLVDPDNRRPVDYTIREEALAALTPRLAKLEGRGEILAELLARW
                     PDGRIKLATTALLLGLRREKESLFAKGAYEPIATGSDRAFGYMRSDGADRAVALMARF
                     PGLREAEPDWGEAAVDLPEGAWSDELTGRRLRGGRVALAQLFAILPVAVLRQG"
     gene            5032..7188
                     /gene="glgX"
                     /locus_tag="VKU03_08275"
     CDS             5032..7188
                     /gene="glgX"
                     /locus_tag="VKU03_08275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012496695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004133 - glycogen debranching enzyme
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen debranching protein GlgX"
                     /protein_id="HLJ71298.1"
                     /translation="MSAILERVSEGKPYPLGATADASGANFAVFSAHATKVEVCLFES
                     FDGRETERFELPEYTDEVFHGHIAGIGPGAFYGFRAHGPYEPEAGHRFNPNKLLLDPY
                     ARAHAGSLVWDHAIFGYTIGAEGDDLTFDERDSAPFVPKCVVIDSNFDWRGEIRRRSI
                     PWNRTILYEMHPKGFTKLHPEVDESLRGYYAGLAAKPVIDYIRSLGITTVELLPVHTF
                     VDDSHLLDKGLRNYWGYNSIGFFAPDPRYASDVANSLREFKEMIARFHDAGLEVVLDV
                     VYNHTAEGNERGPTLSFKGIDNASYYRLAPDQRRYYINDTGTGNTLNLSHPRVIQMVC
                     DSLRYWASEMRVDGFRFDLGTILAREPDGFQTESGFLKAVGQDPLLTEVKLIAEPWDC
                     GPGGYQVGGFPPGWAEWNDTFRDTARDFWRGMASAKALAPRLCASPDTFDRRGRKPWA
                     SVNFITAHDGFTLRDLVSYNEKHNEKNGEDNRDGHADNRSWNCGAEGPTEDAAIKALR
                     LRQTRNFLATLLLSQGTPMVLAGDEFGRTQDGNNNAYCQDNETSWVDWAIDEDGRALA
                     AFFRKLTGFFHRYPVLRRSRFLTGQVNEAIDVKDVTWIDANGSEMTVAAWDDEKTRCF
                     GMLLDGRGQATGIRRRGGDVTLLIVFNAHHDVVDFKLPRCYDASSWSRIMDTNGPELP
                     DQRFRIGAVYKVTARSMLLFERIAARPRRSRIHRET"
     gene            complement(7214..>8895)
                     /locus_tag="VKU03_08280"
     CDS             complement(7214..>8895)
                     /locus_tag="VKU03_08280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016917864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ComEC/Rec2 family competence protein"
                     /protein_id="HLJ71299.1"
                     /translation="DFARDAFFAGVGAVGSTFGAVVKIAPPRDAPPALRFHAAIDRMR
                     NALALRVNAIIGGDEGAIAAAMVTGKRDFLSDDAKDLIREAGIFHIITISGLQMTLVA
                     GIFFWTARRLLALSPTLALNHPIKKWAAAIAMVGAFAYDLCTGSRVGTERALIMTLIV
                     FGAVIVDRRALTMRNLALAVLVVVALEPEAVLGVSFQLSFAAVGALIAVLEARLDRLE
                     PGDDPFLPKSATPATAGLARRIIEKPLALLAATLFATSATASFMAYHFHDLSPYVLIG
                     NPLTLTIIEFFAVPGALIGTALYPLGLDQPVWLYVGLGIRFILWVARHIAAAPGSTLH
                     LRAFAPYALPCLSLAVASALIWRTWLMRLTAAPFLVLGLFGAISGPRYDVIVPPGGDQ
                     AAIRDADGRITVIGKRFNAYAASQWLAADGDGRDPAEARAVDQLCDRLGCAGALPQGL
                     WLSLVDNRLAFDEDCGRALVIVTRLTAPADCRAAVILDEKRLAATGAVGLVIGEDGKV
                     ALAADRGVDQDRPWSPAPRPARNDRVERPGATGKPVRPDPADPPIETEKQP"
BASE COUNT         1544 a         2951 c         2912 g         1488 t
ORIGIN      
        1 tatctggttc gcgtcccgtc gatcctcctg cccaatctca ttctcggcga acgcgctatc
       61 cctgaaatcc tgcagggcga gtgcaagccg gagcgtctcg ccggtctgct cggcgcgctg
      121 atccgcgaca gcgccgagcg ccaggcgcaa ttggcggcgc tcgcccggct cgataagcgg
      181 atgcggctcg atacgggcga gacgccgagc gcgcgcgccg cgcgtctcac gcttgagacc
      241 gtcgaagcgg ccgtccgata aggccgctcc cgcgccgatc gcggcgaagg cgctgcgggg
      301 acaatggaac cggccgcctg tgaccggcgt tgaacctgtc ggtttcacgc cagcggaaat
      361 ttggtgagcc tccttcatcc cgacgacgtc gcaaagctgg aatctgcccg ccgatcgccg
      421 cccgagtcgg cgcttggcgc gcctttcggc gcgacggcgg cgcccgaggg cgtctttttt
      481 cgtctctggg cgccggcttg tgacaacgtt tcgcttctcc tcgaaagcga aaccgagccg
      541 cggccgatgc agccgcgccc gggcggcttc ttcgaagcgt tcgtcccagg gttcggggcc
      601 ggaacgctct atcgctatct cttgccgaac gggatgaaag tccccgatcc ggcttcgcgc
      661 tttcagccgg gcgatgtcga cgggccgagc gaagtcatcg accccgccgc ctatgcctgg
      721 agccaaagct gggccggccg tccttgggac gatatcgtac tgtacgagct ccatgtcggc
      781 gccttcacgc cggaagggac gttccgcgcc gccgccgaaa agctcgacca cttggcgcag
      841 ctcggcgtca ccgcgatcga gatcatgccg gtcgccgatt tccaagggcg ctggaattgg
      901 ggctatgacg gcgcgctgct ctatgcgccg gattcgagct atggccggcc ggaggacttc
      961 aaatctttcg tcgaagccgc ccatgcgcgc ggcgtcgcgg tgctgctcga cgtggtctat
     1021 aaccatttcg gccccgccgg caattatctg cggctctatg cgccgtcctt cttcaccgac
     1081 cgccataaga cgccctgggg cgacgcaatc aactatgacg gcgaggattc gcgcccggtc
     1141 cgggacttcg tcatcgccaa tgcgcaatac tggatcgacg agtttcatct cgacggcctg
     1201 cgcctcgacg ccgtacacgc gatcgccgac gacagtccca agcatattct cgacgaattg
     1261 gcggagcggc tgcgcgcgac cgcgaaacgg ccgctgcatc tgatcgtcga ggacgagaac
     1321 aacgagccgc gccgcttgcg ccggcacggc cgcgcgccga tcctttttac ggcgcaatgg
     1381 aacgacgacg tccatcacgt cctgcatgtc gcggcgaccg aagagcgcgc gggatactac
     1441 gaaggctatc tgaaagacgg cgagctgctc ggcaaagcgc ttgccgaagg gttcgcctat
     1501 caggggcagg tgatgccgta tcgcggccgg gcgagaggcg cgccgagcgc cttcctgccc
     1561 cccggcgcct tcgtcgcctt catccagaac cacgatcagg tcggcaaccg cgctttcggc
     1621 gaaaggctga acatgatcgc gcggccggaa gcgatgcgcg ctttggcggc ggtttatctg
     1681 ttgctgccgc aagcgccgat gatcttcatg ggcgaagaat ggggcgccga gcagccgttc
     1741 ctgttctttt gcgatttttc cggcgatctc gccgaagcgg ttcgcgaagg gcggcgcgcc
     1801 gaattcgccc gctttccgga attcgccgat ccggcggcgc gcgacaagat tcccgatccc
     1861 gtcgccgaga gcgcgttcct cgcgtccaag ctcgactggc gccgaatgaa tcagaaccgg
     1921 ctcgcccatt atcgcgcgct gctgaaaacg cggcggcgct gtatcgcgcc gcttctgccg
     1981 catattcaaa gcggcggccg cgccgtgatt ttcggccccg aggccgtgcg ggttcgctgg
     2041 caggcgcgcg agcgcatcct gatcctcgac gccaatctct gcggccggcg cagcgacttt
     2101 ccgccggcgc gcggccgcat cttctggagc gaaggcgaga cggccggcgc gctcgggccg
     2161 tggtcggtgc ggtggagcgt ggaagcggaa tgaccgcgcc gcgcgcgacc taccggctgc
     2221 aattccacgc gggcttcacc ttcaggcgcg ccgccgccat cgcgccgtat ctcggccggc
     2281 tcggcgtcag ccatgtctat gcttcgcctt acctcaaggc gcggccgggc agcgcgcatg
     2341 gctacgacat cacggatcac aacgccctca atccggaaat cggctcggaa gacgatcacg
     2401 ccgcgatgat cgaagcattc aagcgcgagg gtctgggcct catcctcgat ttcgtcccca
     2461 accatatggg cgtcggcggc gccgacaatc cgttctggct cgacgtgctc gaatggggcc
     2521 aggattccaa ctatgccggt tggttcgaca tcgattgggc ctccgacgat ccctatcttg
     2581 gcgataagct cctcgcgccc tttctcggcg accaatatgg cgtcgagctg aaagcgggca
     2641 agctcgcgct caaatacgac gcggaggagg gcgcctttgc gatatgggcg tacgattatc
     2701 acaaactgcc ggtctgcccg cttcattacg gactcattgt cggcgccgag acgccggggc
     2761 tcgaacggct cggcgatctc ttcggcgatc tcaggcaatg gcggccacag atcgccgagc
     2821 gcgccaagac gctcagggcc gagcttgccg cccatattcg cgccgacgag caggcgcgcc
     2881 aggcgctgac cgcgcggatc gcggcgttca acgccgattg gcgcgccttg gacggcctga
     2941 tcgcgaagca aaattggcgg atcgcctatt acggcgccgc cagcgacgag atcaactatc
     3001 ggcgcttctt caacatcaac gagcttgccg gcctgcgcgt cgaattgccg gccttgttcc
     3061 ggcgcatgca tgcgcgcgtg ctggcgatgc tcgaggacgg cgcgattgac ggcctgcggc
     3121 tcgaccatgt cgacggcctg ttcgatccca aagcctattt cgaggcgctc cgcaaatcgg
     3181 ccgcgcggcc cttctatctc gtggtcgaaa agattctcgc gccgcacgag caattgcggg
     3241 aggaatggcc ggtggacggg acgaccggct acgactacgc caatctctct ctcggcgtct
     3301 tgatcaacgg cgcggcggag gaaagcttct cgcagaccta tcgcgatttt gccggcgccg
     3361 ccgcgccgtt cgatgagatc gcccgcgcct gcaagctgcg catcatggac aatgagatgg
     3421 cgagcgagct caacgcgctg tcgcgcgccg gggttcggct ggcgcatcaa aatccgctga
     3481 ccacggattt ctcgcgaaac attctcaagc gcgcgattcg cctcatcgtc gccagcctgc
     3541 cggtctaccg tacctatatc gacatgagcg gcgcgccgag cgaggcggat ttgcgcgatc
     3601 tcgattgggc ggtcgcccag gcgcgacgcg aagaccccga tatccatcct tccgtcttcg
     3661 cctttcttcg cgcggccctg tccggcgcac tggtcgcggc gccgaacagc ggctatagcc
     3721 gcacggcttg cctgcgcttc gcgatgaagc tgcagcaata ttccggaccg gtcatggcca
     3781 agggcgtcga ggacacggcc ttttaccgct ataaccggca tattgcgctg aacgaggtcg
     3841 gcggcgcccc cgagcggttc ggcgtctcga tcaacgcctt tcacaaggcg aatgccgtca
     3901 gggccaagaa ctggccgcgc gccatgctgg cgactacgac gcatgatacg aagcgcggcg
     3961 aggatgggcg ggcgaggctc gccgttcttt cggacattcc cgaggaatgg cgtcgcgagg
     4021 tcaatgcgtg gagccgcatc attcgggccc gccgcggcga tgtcgagcgg caggcgccgc
     4081 cggatcgcaa cgacgaatac atgttctatc agctcctgat cggctcgtgg ccggccgagc
     4141 tgatgctcga gctcgacgaa ggcgctttgg agaagtaccg gcagcgcgtg cttggcgctc
     4201 tggaaaaatc tctgcgcgag gccaagctcc atacgagctg gacggcgccg aatcccgact
     4261 acgaggaggc gatgcggagc tttgcgcttg acgcgctgga tctcttgcgc agcggattcc
     4321 tgaccaattt ccttcctttc gtccggcgca tcgcgcggct cggcgtcgac aacagtctcg
     4381 cgcaaaccgc gatgaaattg acgctgccgg gcgtcccgga tttctatcaa ggctcggagc
     4441 tatggaatct cagccttgtc gatcccgaca atcgccgccc ggtcgactac actatccgcg
     4501 aggaggcttt ggctgcgctc acgccgcgcc tcgccaagct cgaaggccgc ggcgaaatcc
     4561 tcgccgaact gctggcgcgc tggccggacg ggcggatcaa gctggcgacg acggctctcc
     4621 tgctcggcct gcggcgcgaa aaagaatcgc ttttcgccaa gggcgcctat gagccgatcg
     4681 cgaccggctc ggaccgcgcc ttcggctaca tgcgatcgga cggcgccgat cgggcggtcg
     4741 ccttgatggc gcgcttcccc ggcttgcgcg aggcggagcc cgactggggc gaggcggccg
     4801 tcgatctgcc ggaaggcgcc tggagcgacg aattgaccgg ccggaggctg cgcggcggcc
     4861 gggtcgcgct ggcgcagctc tttgcgatcc tgcccgtcgc cgtcttgcgg caaggctgac
     4921 gccgcgcttg cgccgccggc ggcgcgccca tcaggaacag gaaagcgggg tacggcgttt
     4981 aagagtttcg ggcgtacagg aagaacagac aacgccgaaa cggggtgaga cttgagcgcg
     5041 atcttggagc gggtcagcga aggcaagcct tacccgctcg gcgcgacggc ggacgccagc
     5101 ggcgcgaatt tcgcggtgtt ttccgcgcac gcgaccaaag tcgaagtctg cctcttcgag
     5161 tcgttcgacg gccgcgagac cgagcgtttc gagcttcccg aatataccga cgaggtcttc
     5221 catggccata tcgccgggat cgggccgggc gccttctatg gcttccgcgc gcatggccct
     5281 tacgaacccg aagccggcca tcgcttcaat ccgaacaagc tgctgctcga tccttacgcg
     5341 cgcgcccatg ccggatcgct cgtctgggac catgcgattt tcggctacac gatcggcgcg
     5401 gagggcgacg atctgacgtt cgacgagcgc gatagcgcgc cgttcgtgcc gaaatgcgtg
     5461 gtcatcgatt cgaatttcga ctggcgcggc gaaatccggc ggcgctcgat cccctggaat
     5521 cgcaccatcc tttacgagat gcatccgaag ggcttcacca agctgcatcc cgaggtcgac
     5581 gaaagtctgc gcggctatta cgcgggcctc gccgccaagc cggtgatcga ctatatccgc
     5641 tcgctcggga tcacgacggt cgagctcctg ccggtccata cattcgtcga cgacagccat
     5701 ctgctcgaca agggcctgcg caattactgg ggctacaatt cgatcggctt tttcgcgccc
     5761 gatccgcgct acgcatccga cgtcgcgaac tccctgcgcg agttcaagga gatgatcgcc
     5821 cgctttcatg acgccggcct cgaagtcgtt ctcgacgtcg tctataatca tacggccgag
     5881 ggcaacgagc gcgggccgac tttatctttc aagggcatcg ataacgcgtc gtactatcgg
     5941 ctggcgcccg accagcgccg ctactacatc aacgacacgg gcacgggcaa cacgctcaat
     6001 ctcagccatc cgcgcgtcat ccagatggtc tgcgacagcc tgcgctattg ggcgagcgaa
     6061 atgcgcgtcg acggatttcg cttcgatctc ggcacgatcc tcgcgcgcga gcccgacggc
     6121 ttccagactg agagcggatt cctcaaggcg gtcgggcagg acccattgct gacggaggtc
     6181 aagctgatcg ccgagccctg ggattgcggc ccaggcggtt atcaggtcgg cggcttcccg
     6241 ccgggctggg ccgagtggaa cgacacgttt cgcgacacgg cgcgggattt ctggcgcggc
     6301 atggcgagcg ccaaagccct ggcgccgcgc ctctgcgctt cgcccgacac attcgaccgc
     6361 cgcggtcgca agccatgggc ctctgtcaat ttcatcaccg cccatgacgg attcacgctg
     6421 cgagatcttg tctcgtacaa cgagaagcac aacgagaaga acggcgagga caatcgcgac
     6481 ggccatgccg acaaccggag ctggaattgc ggcgcggaag ggccgaccga ggacgcggcg
     6541 atcaaggcgc tccgcttgcg ccagacgcgc aatttcctgg cgacgctgct tctttcgcaa
     6601 ggaacgccca tggtcctggc cggcgacgag ttcggccgca cgcaggacgg caacaacaac
     6661 gcctattgcc aggacaatga gacgagttgg gtcgattggg cgatcgacga agacggccgc
     6721 gcgctcgccg ccttctttcg caagctgacc ggctttttcc atcgctatcc cgtcttgcgg
     6781 cgctcgcggt ttctcaccgg ccaggtcaac gaggcgatcg acgtcaagga cgtgacctgg
     6841 atcgacgcca acgggtcgga gatgaccgtc gcggcttggg acgacgagaa gacgcgctgc
     6901 ttcggcatgc tgctcgacgg ccgcggccag gcgaccggca tccgccggcg cggcggcgac
     6961 gtgacgctgc tgattgtctt caacgcccat cacgacgtgg tcgatttcaa gctgccgcgc
     7021 tgctacgacg cctcaagctg gagccgcatc atggacacga acgggccgga actgcccgat
     7081 cagcgattcc gcatcggcgc cgtttacaaa gtgaccgccc gctcgatgct gctgttcgag
     7141 cggatcgcgg cgcggccgcg ccggagccgg atccatcgcg agacctgatg gcgcgcgcga
     7201 gagtgcgccg acctcacggc tgcttttcgg tctcgatcgg aggatcggcg ggatcgggcc
     7261 tgaccggctt gcccgtcgcg cccggccgct ccacccgatc gttgcgcgcc ggccgcggcg
     7321 ccggcgacca gggccgatcc tgatcgacgc cgcgatccgc cgccagcgcc acctttccgt
     7381 cctcgccgat gacgagaccg accgcgccgg tcgccgcgag ccgcttctcg tcgagaatca
     7441 ccgcggccct gcaatcggcc ggcgcggtca ggcgggtgac gatgaccagc gcgcggccgc
     7501 aatcctcgtc gaaagcgagc cggttgtcga ccagcgacag ccataggcct tgcggcagcg
     7561 cgccggcgca gccgagccgg tcgcaaagct gatcgacggc gcgcgcctcg gccgggtcgc
     7621 ggccgtcgcc gtcggcggcg agccattggc tggcggcata ggcgttgaac ctcttgccga
     7681 tcaccgtgat ccggccgtcg gcgtcgcgga tcgccgcctg gtcgccgccg ggcggcacga
     7741 tcacgtcgta acgggggccg ctgatcgcgc caaacaagcc caggacgaga aacggcgccg
     7801 cggtcagccg catcagccag gtccgccaga tcagcgccga ggcgacggcc aggctcaagc
     7861 agggcagggc gtaaggggcg aaggcgcgca aatgcaaagt cgagcccggc gccgcggcga
     7921 tgtggcgcgc gacccagaga atgaaccgga tgccgagccc gacatagagc cagaccggct
     7981 gatcgaggcc gagcggataa agcgcggtcc cgatcagcgc gccgggcacg gcgaagaatt
     8041 cgatgatggt cagcgtcaac ggattgccga tcagcacata ggggctgaga tcgtggaagt
     8101 gataggccat gaagctggcc gttgcgctgg tcgcaaagag cgtcgcagcc agaagcgcga
     8161 gcggcttttc gatgatgcga cgggccaagc cggcggtcgc cggcgtcgcg ctcttcggca
     8221 gaaacggatc gtcgcccggc tcgagacgat cgagccgcgc ttccaggacc gcgatcaagg
     8281 cgccgaccgc ggcgaaggag agctggaagc tgacgccgag cacggcctcc ggctcaagcg
     8341 cgacaacgac gaggacggcc aaagcgagat tgcgcatggt caaggcgcgg cgatcgacga
     8401 tcactgcgcc aaacacgatc agggtcatga tcagggcgcg ctcggtgccg acgcgcgagc
     8461 cggtgcaaag gtcgtaggcg aaggcgccga ccatggcgat cgccgccgcc catttcttga
     8521 tcggatggtt caaggcgagg gtcggcgaca gcgcgagaag gcggcgcgcc gtccagaaga
     8581 aaatgcccgc caccagcgtc atctgcaggc cggatatggt gatgatatgg aaaatgccgg
     8641 cttcgcggat caggtctttc gcgtcgtcgg agagaaaatc gcgcttgccg gtgaccatcg
     8701 cggcggcgat cgcgccctca tcgccgccga tgatcgcatt gacccgcagc gcgagcgcat
     8761 tgcgcattcg atcgatcgcc gcgtgaaagc gcaaggccgg cggcgcgtcg cgcggcggcg
     8821 cgatcttgac caccgcgccg aaagtcgatc cgaccgcgcc gacgcctgcg aaaaacgcgt
     8881 cgcgcgcgaa atcgt
//
LOCUS       DATGJW010000265         3387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_50584, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000265 DATGJW010000000
VERSION     DATGJW010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3387
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50584"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            319..1881
                     /locus_tag="VKU03_08285"
     CDS             319..1881
                     /locus_tag="VKU03_08285"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY family carbohydrate kinase"
                     /protein_id="HLJ71300.1"
                     /translation="MAGRGAFDGPVLLGVDVGTQSIRALAFAAGGDHLATASRPTPSR
                     PYGAGGADYDPDELFATVEACLREIVNALPQGAPVAGLAVASVGETMVLVGADGRAAT
                     PALAWFDRRTEQAAQAIEKRIGGARLFELTGHRLDPTLSLCKLWWMRETWPDAFAAAT
                     RALNVADWIAYRLTGVAAADFTLASRTLALDIKARRWSAEILDALGFDRGLFAPIKPN
                     GAPLGPVRADLSAALGFAKPPIVGVGGHDHLCGLFAAGAARPGALLDSMGTAEAILRA
                     TERPLIDARVDDQGFIQGAIGATRDLFYLGGAIYSSGGAVEWLRDLYGGVSRDVLIAE
                     ARAAPPGGGGIVFLPHLAYAGAPEPDVQARGAFIGLSGRSTRAMLYRAALEGLSLQVR
                     RMSEAIAALPGIGPPREIRIIGGSSRNPLFQSIKASVLGAPIMVMDEAESTALGAALL
                     GGVAAGLWPDLDAAAAGLEGRFHIVEPEPSWVAFYAELYEKVFRSLQASLKPANNHIA
                     ILEAKAALRGQT"
     gene            1969..2925
                     /locus_tag="VKU03_08290"
     CDS             1969..2925
                     /locus_tag="VKU03_08290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917892.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ71301.1"
                     /translation="MGASAALAGLAPLRAAAAGKKLTIALIPGLTTDAFYITMHRGAD
                     AAAKALGVDLVFQGAPQWGVTLQVPVLQAVAARKPDAILIAPTDKQQLIEPLKQAFDA
                     GITIVTVDTFIGNGVYQTGSGDADFPISYVASDNILGGRIAARALAKAIGDKGKVFVA
                     NTIPNVSTTDQREEGFKAEMKSHPNIQVLQTQFNDDDANKAAALAQAVLAREPDLAGI
                     FGANLFSATGSANGVRAAGKVGKVKIAAFDAPADIVDHLKDGTFEIAIAQHPAEIGYF
                     GVVCAVAHLTGNSVPALIGTGFTVMTKDNIDDPNVKKYVYTG"
     gene            3000..>3387
                     /locus_tag="VKU03_08295"
     CDS             3000..>3387
                     /locus_tag="VKU03_08295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter"
                     /protein_id="HLJ71302.1"
                     /translation="MTAIPAPRSTRARGVLTWISNLRAWLFLIFLLIFFEVWAETIYG
                     SSFVANLYNWQSITVFATAPLLLALGQTLVIISSGIDLSVGFTMGLSAVVAAHISNIC
                     IDYARLPPAAAAFLAILAGIGVSLVPG"
BASE COUNT          547 a         1135 c         1112 g          593 t
ORIGIN      
        1 cccagggcga cggcgcctcg gggttgagcg cgagcgaggg caaagtcggc tcggcaggcg
       61 ttgaactggt ctgagcttcg gcgcggccgc ccgcccataa gacgccgcat acggcgaggg
      121 ccgttaacaa tctcagcgcc atgcgaccct catggatttg tcgttggcaa tcatattcag
      181 caggcaagtg cggcgtcaaa gcggcggtcc gggcgaaagg cgtttatgcg caggcgcggc
      241 gcttgccaag cgaaagcccg ccggaaaaga attgcgcgcg gcgcgtcggc gcgcctcgga
      301 gcaaagcgag ttcggagcat ggcgggacga ggcgcgttcg acggacccgt tctgcttggc
      361 gtcgatgtgg gaacgcagtc gatccgcgcc ctcgctttcg ccgcaggcgg cgaccatctg
      421 gcgacggcga gccggccgac gccgagccgc ccttacggcg cgggcggcgc cgattacgat
      481 ccggacgagc ttttcgccac ggtcgaggcc tgtcttcgcg aaatcgtgaa cgcgctgccg
      541 cagggcgcgc cggtcgccgg cctcgccgtc gccagcgtcg gcgagacgat ggtgctggtt
      601 ggcgcggatg gccgcgccgc gacgccggcc ctcgcctggt tcgatcgccg caccgagcag
      661 gcggcgcaag cgatcgaaaa gcggatcggc ggcgcgcggc tgttcgaact taccggccat
      721 cggctcgatc cgacgctgag cctctgcaag ctgtggtgga tgcgcgaaac ttggcccgac
      781 gccttcgccg ccgcgacgcg cgcgctgaat gtcgccgatt ggatcgccta ccgtctgacc
      841 ggcgtggcgg cggccgattt cacgctcgcc tcccgcacgc tcgccctcga catcaaagcg
      901 cggcgatggt cggcggagat tctcgacgcg ctgggattcg accgcgggct cttcgcgcca
      961 ataaagccga acggcgcgcc tctcgggccg gtacgagccg atctctcggc ggcgctcggc
     1021 ttcgccaagc cgccgatcgt cggcgtcggc ggccatgatc atctctgcgg cctgttcgcc
     1081 gccggcgcgg cgcggccggg cgcgctgctc gacagtatgg gaacggccga agcgatcttg
     1141 cgcgcgacgg agaggccgct gatcgacgcc cgcgtcgacg atcagggctt cattcaaggc
     1201 gcgatcggcg cgacgcgcga tctcttctat ctcggcggcg ccatctatag ttcgggcggc
     1261 gccgtcgaat ggctgcgcga tctttacggc ggcgtttcgc gcgacgtgct gatcgccgaa
     1321 gcgagggccg cgccgccggg cggcggcgga atcgtcttcc tgccgcatct cgcctatgcc
     1381 ggcgcgcccg agccggacgt ccaggcgcgg ggcgccttta tcggcctgag cgggcgttca
     1441 acccgcgcca tgctctatcg cgctgcgctc gaaggcttgt ctttgcaagt gcggcggatg
     1501 agcgaagcga tcgcggcgtt gccgggaatc gggccgccgc gcgaaatccg catcattggc
     1561 ggcagttcgc ggaatccgct gttccagtcg atcaaggcga gcgtgctggg cgcgccgatc
     1621 atggttatgg acgaagcgga atcgacggcg ctcggcgccg ccttgctcgg cggcgtcgcc
     1681 gcgggcctct ggccggatct cgacgctgcc gcggcgggcc tcgaaggccg cttccatatc
     1741 gtcgagccgg agccgtcatg ggtcgctttc tatgccgagc tctatgagaa agttttccgc
     1801 agcttgcagg cctcgcttaa accggccaat aatcacatcg ccattctcga ggccaaggcc
     1861 gctttgcgcg gtcagactta atgcccattg cgggcaaacc agcgtcaatg gcgagccata
     1921 agagagaaaa gggagagaac gatggaacgc cggtcattcc tgtctctaat gggagcttcc
     1981 gcggctttgg cgggccttgc gcctctgcgc gccgccgcgg cgggcaagaa gctgacgatc
     2041 gcgctgatcc ccggtctgac gacggacgct ttctacatca ccatgcatcg cggcgccgac
     2101 gcggcggcca aggcgctggg cgtcgatctc gtctttcagg gcgcgccgca atggggcgtg
     2161 acgctgcaag tgccggtgct gcaagccgtc gccgcgcgca agcccgacgc catcctgatc
     2221 gcgccgaccg acaagcagca attgatcgag ccgctgaaac aggctttcga cgccggcatc
     2281 accattgtca ctgtcgatac gttcatcggc aacggcgtgt accagaccgg ctcgggcgac
     2341 gccgatttcc cgatctccta tgtcgcctcc gacaatattc tcggcgggcg gatcgccgcg
     2401 cgcgcgctcg ccaaggcgat cggcgacaag ggcaaggtct tcgtcgcgaa tacgattccc
     2461 aatgtctcga cgaccgacca gcgcgaggaa ggcttcaagg ccgagatgaa gagccatccc
     2521 aacatccagg tcctgcaaac ccagttcaat gacgacgacg ccaacaaggc ggcggctttg
     2581 gcgcaggcgg tgctcgcgcg agagccggat ctcgccggca ttttcggcgc gaacctgttc
     2641 tcggcgacgg ggtcggccaa tggcgtccgc gcggcgggca aggtcggcaa ggtcaagatc
     2701 gccgctttcg acgcgcccgc cgacatcgtc gaccatctga aggacggcac gttcgaaatc
     2761 gccatcgccc agcatccggc cgagatcggc tatttcggcg tggtctgcgc cgtcgcgcat
     2821 ctgaccggca attccgtgcc ggccttgatc ggcaccggct tcacggtcat gaccaaggac
     2881 aatatcgacg atccgaacgt gaagaaatac gtctatacgg gttgagcctt cctctcgccg
     2941 atcgagcagc ccgtcggcaa ggcgccggcg ggcttttccc ttaaaagccg gtctcgccga
     3001 tgacagccat tccagcgccg aggtctacgc gcgcccgcgg cgtcctgacc tggatctcga
     3061 atctgagagc ctggcttttc ctcatcttcc tcctgatctt cttcgaagtc tgggccgaga
     3121 ccatctacgg atcgagcttc gtcgccaatc tgtacaattg gcaatcgatc accgtcttcg
     3181 ccacggcgcc gctgctcctc gccctcggcc agacgctggt gatcatttcc agcggcatcg
     3241 atctctcggt cggcttcacg atgggcctct ccgccgtcgt cgccgcgcac atatcaaaca
     3301 tctgcatcga ctatgccaga cttccgccgg cggcggcggc gtttctggct atccttgccg
     3361 gaatcggcgt ctcgctcgtt cccggct
//
LOCUS       DATGJW010000266         4883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1087442, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000266 DATGJW010000000
VERSION     DATGJW010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4883
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087442"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2202)
                     /gene="mutS"
                     /locus_tag="VKU03_08300"
     CDS             complement(<1..2202)
                     /gene="mutS"
                     /locus_tag="VKU03_08300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HLJ71303.1"
                     /translation="MMAQYFEIKAANPDCLLFYRMGDFYEMFFRDAEIASAALGIVLT
                     KRGKHEGADIPMCGVPIERADEYLQRLIARGHRVAVCEQLEDPALAKKRGPKAVVRRD
                     VVRLVTPGTITEERLLEPGRANCLLAIHRVRAGEDRTSFGLAAIDISTGAFRLSEADY
                     ASLGAEIARLDPSEIIAAQATLDDSEAARGFAEARAAFTPIGRESGGFESAERTLRDF
                     YGVETLEGFGAFSRAEIGAAALAIDYIGRTQIEARPALERPTRRPRGEAMEIDASTRA
                     NLELARTLSGERQGSLLATIDLTATPAGARLLAERLAGPLTDPAAINNRLDAVGFFLN
                     ESRLRGDLRAALRAAPDIARAMSRLSLDRGGPRDLAALRDGLAAARAVAERLIEQPVA
                     SAIVVAAATRLSRMTPDLERTLAATLGDDLPLNKRDGGFVREGAEPTLDEARRLRDES
                     RRVIAELHGKYSALAETNQIRIKHNNFLGFFIETPQAQGEKLLKAPFSATFIHRQTMA
                     GAMRFSTSELAELEAKIASAADRALALELAAFDRLRQAALAEIEAIRGLGEALAEIDV
                     TCALAELAATRDWVRPLVDDTLNFVIEGGRHPVVEAALSAQGEPFVANDCDLSGDEGG
                     GRIALVTGPNMAGKSTYLRQAALIALLAQSGAYVPARRAHIGIVDRLFSRVGAADDLA
                     RGRSTFMVEMVETAAILNQATQRSLVILDEIGRGTATFDGLSIAFATIEHLN"
     gene            complement(2669..3622)
                     /locus_tag="VKU03_08305"
     CDS             complement(2669..3622)
                     /locus_tag="VKU03_08305"
                     /inference="COORDINATES: protein motif:HMM:NF020132.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLJ71304.1"
                     /translation="MTTRPPVALTIAGSDSGAGAGIQADLKTFAAIGVYGTCALTALT
                     AQNTLGVRDICFVPAQSVAAQIDAVLDDFDVAAIKIGMLGSAEIVEAVAARLGALPSP
                     RPSPASGRGGAARASAALAESSRSAGQSLLPLAGEGGRAEHGRMRARPLVVYDPVMAA
                     SSGDALSQEGFLEAARTKLLPLIDCLTPNLAEAAALLGAAEARDETAMAAQGRALLTL
                     GPRAVLMKGGHLMSREATDILVTPAGARRFTEARIDSANTHGTGCTLSAALAAHVALG
                     AQLEDAIAEAKAFVARAIEAGRDVKLGDGLGPLVQMALRTR"
     gene            complement(3619..4224)
                     /locus_tag="VKU03_08310"
     CDS             complement(3619..4224)
                     /locus_tag="VKU03_08310"
                     /EC_number="2.5.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185585.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004789 - thiamine-phosphate
                     diphosphorylase activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine phosphate synthase"
                     /protein_id="HLJ71305.1"
                     /translation="MTLDPFYPIVDSAAWVRRLLGVGARLIQIRVKDRPRDDTSREIR
                     AALAACRAASAQLVVNDYWDIGIDEGADFVHLGQGDLDGADIKRIRAHGVKLGVSTHD
                     HAELDRGLSLDPDYVALGPIYPTRLKAMAFGPQGLERIGEWRKLVGRRPLVAIGGITL
                     ERAPLCLAAGADIVSVVTDITLNPDPEARAAAWVAATRRPA"
     gene            complement(4436..>4883)
                     /locus_tag="VKU03_08315"
     CDS             complement(4436..>4883)
                     /locus_tag="VKU03_08315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="thiazole synthase"
                     /protein_id="HLJ71306.1"
                     /translation="FGLVEAARILSDEGFAVFPYTTEDLVVAERLLEAGCRALMPWGA
                     PIGSGKGLNNPFGLRALRAHFPDVPLIVDAGIGLPSHAAAAMELGYDAILLNTAVAKA
                     GDPAAMARAFALAIEAGRAAYLAGPMAPRDMAAPSTPVLGKPSLTG"
BASE COUNT          761 a         1652 c         1744 g          726 t
ORIGIN      
        1 gttaagatgc tcgatcgtcg caaaagcgat ggagaggccg tcgaaggtcg ccgttccgcg
       61 gccgatctcg tcgagaatga ccagcgagcg ctgggtcgcc tgattgagga tcgccgcggt
      121 ctcgaccatc tcgaccatga aggtcgagcg gccgcgggcg agatcgtcgg cggcgccgac
      181 gcgcgagaac agccggtcga cgattccgat atgagcccgc cgcgccggaa cataggcgcc
      241 ggattgcgcc agcagcgcga tcaacgcggc ttggcgcaga taggtcgatt tgccggccat
      301 gttcgggccg gtgacgagcg cgatgcggcc gcctccttca tcgccggaaa ggtcgcaatc
      361 attggcgacg aaaggctcgc cctgggcgct gagagctgct tcgaccaccg gatggcggcc
      421 gccttcgatg acgaaattga gcgtatcgtc gacgagcggg cggacccagt cgcgcgtcgc
      481 ggcgagctcg gccagcgcgc aggtgacgtc gatttcggcc aaagcctcgc caaggccgcg
      541 gatcgcctcg atctcggcga gggccgcctg gcgcagcctg tcgaaagcgg cgagctccaa
      601 agccagcgcg cgatcggcgg ccgaggcgat cttcgcttcg agctcggcga gctcgctggt
      661 cgaaaagcgc atcgcgccgg ccatggtctg gcgatggatg aacgtcgccg agaagggcgc
      721 tttcagcagt ttttcgcctt gcgcctgcgg cgtctcgatg aagaagccga gaaagttgtt
      781 gtgcttgatc cggatctggt tggtctcggc gagcgcgctg tactttccat gcagctcggc
      841 gatgacccgc cggctctcgt cgcgaaggcg tcgcgcttcg tcgagcgtcg gctcggcgcc
      901 ctcgcgcacg aagccgccgt cgcgtttgtt gagcggcaga tcgtcgccga gcgtggcggc
      961 gagcgtccgc tccaaatcag gcgtcatgcg ggaaaggcgc gtcgccgccg cgacgacgat
     1021 cgcgctggcg accggctgct cgatgaggcg ctccgcgacg gcgcgcgcgg cggccagccc
     1081 gtcgcgcaag gcggcgaggt cgcgcggccc gccgcgatcg aggctcagcc gcgacatggc
     1141 gcgggcgatg tcgggcgcgg cgcgcagggc ggcgcgcaag tcgccgcgca gccgcgactc
     1201 gttgaggaag aagccgactg cgtcgagcct gttgttgatc gcggcgggat cggtgagcgg
     1261 gcctgccagc cgctcggcga ggaggcgcgc gccggccggc gtcgccgtca ggtcgatcgt
     1321 cgccagcagc gagccttgcc gctcgccgct caatgtgcgc gcgagttcga gattggccct
     1381 ggtcgaagcg tcgatctcca tcgcctcgcc gcgcgggcgg cgggtcggcc gctccagcgc
     1441 cggccgcgcc tcgatctggg tgcgaccgat atagtcaatg gcgagcgccg cggcgccgat
     1501 ctcggcgcgc gaaaaagcgc cgaacccctc cagcgtctcg acgccgtaga aatcgcgtaa
     1561 agtccgctcg gcgctctcga agccgccgga ttcgcggccg atcggcgtga acgccgcccg
     1621 cgcctcggcg aaaccgcgcg ccgcttccga atcgtccagc gtcgcctgcg cggcgatgat
     1681 ctcgctcgga tcgagccgcg cgatttcggc gccgagcgag gcgtaatccg cttcgctgag
     1741 ccggaaggcg ccggtcgaga tatcgatcgc ggcgaggccg aaagaggtac ggtcctcgcc
     1801 ggcgcggacg cgatggatcg ccagcagaca attggctctg cccggctcga gcaggcgctc
     1861 ctcggtgatc gtgccgggcg tcaccagccg gaccacatcg cgtcgcacta ccgctttcgg
     1921 tccgcgcttc ttggcgagcg cggggtcttc gagttgctcg cagaccgcga ctctgtggcc
     1981 gcgggcgatc agccgctgca gatattcgtc ggcgcgctcg atcggcacgc cgcacatcgg
     2041 aatgtcggcg ccctcatgct tgccgcgctt ggtgaggaca atgccgagcg cggcgctggc
     2101 gatttcggcg tcgcggaaga acatttcata gaaatcgccc attcggtaga agagcaggca
     2161 atcgggattg gccgccttga tctcgaaata ttgcgccatc atcggcgtga gcttgaggtc
     2221 ggcctcgcgc ggagcgggcg cggcgtcgct ctcgggcggc tggatgtggc gggtcatcgg
     2281 cggagcgtag caaagcgggg cgggcggcgc atcggcggga ggggcgctgt ccacgggaat
     2341 ggggcggggc agggcgcgtg gcggagtggc caattgcccg gccccgcgct gcctgagccg
     2401 cgtgcggccg taaggccaag cgcttcgcgt cgcgagccaa gaccgcctca gaagaaagcg
     2461 ccgcgccatg gctcgcgagc cttgaccgcc gtccgcggct caggctcgtt ggcgaccaag
     2521 caacaaagcc gcatcgggcc ggcgttgtca cccgaagggc cgaagcgaag cagtgtccat
     2581 agccgtcgct cggcggcgga atcaaaggcg ctgtatcgcc gacggctatg gacacttgac
     2641 agcgccggtc cgatgtggcg cctccgcgct atctcgttcg cagcgccatc tgtaccaacg
     2701 gcccaagccc gtcgcccagc ttcacatcgc gccccgcctc aatcgctcgc gcgacaaacg
     2761 ccttcgcctc ggcgatggcg tcctccaatt gcgcgccgag cgcgacgtga gccgcgagcg
     2821 cggccgacag ggtacagccg gtgccatgcg tattcgccga gtcgatccgc gcttccgtaa
     2881 agcgccgcgc ccccgcaggt gtgacgagga tgtccgtcgc ctcgcgcgac atcaggtgtc
     2941 cccccttcat cagaaccgcc cgcggcccga gcgtaagcag cgcgcgcccc tgcgccgcca
     3001 tcgccgtctc gtcgcgagcc tcggcggcgc cgagcaaggc cgcggcctcg gcaagattgg
     3061 gcgtcaggca atcgatcaat gggaggagct tcgttcgtgc agcctcaaga aagccttctt
     3121 ggctcagcgc atctcctgaa gaggcggcca tcacgggatc gtaaacgacg agcggccgcg
     3181 ccctcatccg gccgtgctcc gcgcgtccac cttctcccgc aagcgggaga agggattggc
     3241 ctgcactccg tgaagattcc gccaatgcag ccgacgcgcg cgccgcgccc cctctcccgc
     3301 ttgcgggaga gggtcggggt gagggtagag cgccgagccg agcggcgacc gcctcgacga
     3361 tctcggccga accaagcatg ccgatcttga tcgccgccac atcgaaatcg tcgagcacgg
     3421 cgtcgatctg cgcggccacg gactgggcgg gtacgaaaca gatatcgcgc acgcccagtg
     3481 tgttctgcgc tgtcagcgcc gtgagcgcgc atgttccgta gacgccgatc gccgcgaatg
     3541 tcttcaggtc cgcctggatt cccgcgccgg cccccgaatc cgagccggct atggtgagcg
     3601 cgaccggcgg gcgcgttgtc acgccgggcg ccgcgtcgcc gcgacccagg ccgcggcgcg
     3661 cgcctccgga tcgggattga gcgtgatgtc ggtgacgacc gagacgatat cggcgcccgc
     3721 ggcgaggcag agcggcgcgc gctccagcgt gatcccgccg atcgcgacga gcgggcggcg
     3781 gccaacgagc ttcctccatt cgccgatccg ctccagcccc tgcgggccga aggccatcgc
     3841 tttcagccgc gtcggataga tcgggccgag cgcgacatag tcggggtcga gagagaggcc
     3901 gcggtcgagc tcggcgtgat catgcgtcga gacgccgagc ttcacgccat gggcgcggat
     3961 gcgcttgatg tcggcgccgt cgagatcgcc ttggccgaga tggacgaaat cggcgccttc
     4021 gtcgatgccg atgtcccaat agtcgttgac gacaagctgc gcgctggccg cgcggcatgc
     4081 ggcgagcgcc gcgcggattt cgcggctcgt gtcgtcgcgc ggacggtctt tgacgcggat
     4141 ttggatgagc cgagcgccaa cgccgagcag gcggcgcacc cacgcggcgc tgtcgacgat
     4201 cggatagaag ggatcgaggg tcatgccgcg cggccgggaa accccttctc cccgcttcgc
     4261 gggaagaagg cggccgcgaa gcggccggag gaggggcggc gccggactct gaagtcacgc
     4321 acccttcgaa acgccaatcg gcgagctcgg cgcagcccct catccggcgc ttcgcgccac
     4381 cttctccccg cgttgcgggg agaagggaat aatcccagcg cctcgtcaat catcctcacc
     4441 ccgtcaggct cggcttgccc aggacgggcg tcgacggcgc ggccatgtcg cgcggcgcca
     4501 tcgggccggc gagatacgcg gcgcggccgg cttcgatggc gagggcgaag gcgcgggcca
     4561 tggccgccgg atcgccggct ttggcgacgg cggtgttcag caggatagcg tcgtagccga
     4621 gctccatcgc cgccgcggcg tgcgagggca ggccgatgcc ggcgtcgacg atcaagggca
     4681 cgtccgggaa atgcgcgcgc aaggcgcgca ggccgaaggg attgttcaag cccttgcccg
     4741 atccgatcgg cgcgccccac ggcatcagcg ctcggcagcc ggcttcgagc aggcgctcgg
     4801 cgacgacgag gtcctcggtc gtgtagggaa agacggcgaa gccctcgtcg ctgagaatgc
     4861 gcgcggcctc gaccaggccg aac
//
LOCUS       DATGJW010000267         5043 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_50678, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000267 DATGJW010000000
VERSION     DATGJW010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5043)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5043)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5043
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50678"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1108
                     /gene="thrC"
                     /locus_tag="VKU03_08320"
     CDS             <1..1108
                     /gene="thrC"
                     /locus_tag="VKU03_08320"
                     /EC_number="4.2.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004795 - threonine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009088 - threonine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="threonine synthase"
                     /protein_id="HLJ71307.1"
                     /translation="VLELFHGPTLAFKDFAMQWLGRAMNRALKARGARATIVGATSGD
                     TGAAAIEAFAGQSQTDIFILYPHNRVSDVQRRQMTTIDQPNVHAIAVEGSFDDCQAIV
                     KGLFNDLSFRDAHHLTGVNSINWARIVAQIVYYFTSAVTLSAPHRSVSFTTPTGNFGD
                     ILAGWCAKRMGLPIKRLVIATNANDILARALASGEYRAREVIATQSPSMDIQVSSNFE
                     RLLFDAYGRDAAALRGLMANFAQSKSFAIAPGPLKAIRDDFDAFATDEETCIEEMART
                     YREAGYPLDPHSAVGVNAARRALARDPSTPMIALATAHPAKFPEAVARATGGRPALPD
                     HLKGLLEKKERFAILPNDLGAVRQFIRARARAAA"
     gene            1105..2367
                     /locus_tag="VKU03_08325"
     CDS             1105..2367
                     /locus_tag="VKU03_08325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HLJ71308.1"
                     /translation="MSVEITKLESGLRVVTDAMPQLRTASLGVFVGAGSRDERPQEHG
                     LSHLLEHMAFKGTRRRSARQIAEEIENAGGDLNAETGVEQTAYFARVLGEDCELALDI
                     LSDILTDSQFDAAELEREKGVILQEIGAVEDAPDDLVFDLFTETAWPEQAIGRPILGT
                     RERVRAFDRAAIDLYLRRHYQAGATIVAAAGAVEHRRIVDLAERLFATLGKGDFPAAP
                     PALYRGGESMTKKGLGQTHIVVGFEGRAAHDADHDAAHVFAAATGGGMSSRLFQEVRE
                     KRGLAYSIYAFHWAYSDSGLMGFYAGTSPADAGALMEAALDCLAEASHSLSEEEVHRA
                     KAQMKVSLLAALESSGARAQQLARHILIYGRPLTVEEMTARIDRLTVADIRKAGAAML
                     RSAPTVTAIGRVKKVLDQDRVARRLGGV"
     gene            2375..2989
                     /locus_tag="VKU03_08330"
     CDS             2375..2989
                     /locus_tag="VKU03_08330"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HLJ71309.1"
                     /translation="MALFRINRLADPAPLARGDGLYLRPAQSVDYAAWAALREASRAF
                     LTPWEPSWPADDLTRAAFRRRLKRQAEEIARDEAYSFLIFEEGSDDMLGGITIGGVRR
                     GVAQAATLGYWIGAPYARRGHMTRAVAAATAFGFSSLRLHRIEAACIPDNVASARVLE
                     RNGFRKEGFARAYLRINGAWRDHLLFALLESDARAIQQGGRGVG"
     gene            2982..>5043
                     /locus_tag="VKU03_08335"
     CDS             2982..>5043
                     /locus_tag="VKU03_08335"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diguanylate cyclase"
                     /protein_id="HLJ71310.1"
                     /translation="MVKRWARLALLFLFLGLFSQAAFAVQSVRVPIDADAIDLGSAVE
                     HYSAQGDRIQVSTAPGADGIVRRIEVSALEAGSQASWIVFALTNDTDEQLTRLIVAPH
                     FRLVGSGLIWPDLGSSRITTITASQGEAPEREDHPDADVFRLTLDPGATVTYVAELRT
                     PNLPQIYLWEPDAYKDKVASLTLYQGIVIGIAGLLALFLTIVFVVRGAVIFPAAAALA
                     WTVLAYVCIDFGFWRKIFGFEDEAERIWRAGVEATLAATLIIFLFAYLNLRRWHVRYS
                     HIAAIWLFLMAAVIGLSVYNAPIAAGVARISIATAAGIGFILILNMAARGSDRAVMLI
                     PAWFLLLVWVVGAGFTVTGALTNDLAAPGLIGGLVLIVMLIGFTVMQNAFAGSGLSSI
                     RGPDSERRALAMTGSGDAIFDWSVESDQIYVSPEIENQLGLPRGALQGPATSWLDMLH
                     PMDRDRYAAVLDGLLQQRCGRVNHDLRLRAADGQYFWYVLKARPVVNADGEVMRVIGS
                     LADVTEMKTAEERMLHDAVHDNLTGLPNRELFFDRLESALIAAKRPGGPRPAVMAIDV
                     DRFKQINEAIGLSYGDSVLLTVARRLGRNLRPGDTLARIAGDQFGAIVMTEDEADNIL
                     DFVDTTRNILSTPISFGDREVALTVSIGVALFDPNAHAKGGDILKDAEIALAHAKKSG
                     GDRAE"
BASE COUNT          797 a         1715 c         1629 g          902 t
ORIGIN      
        1 cgtcctcgag cttttccatg gcccgacctt ggcgttcaag gatttcgcca tgcaatggct
       61 cggccgggcg atgaaccgcg cgctgaaagc gcgcggggcg cgggcgacga tcgtcggcgc
      121 gacgtcgggc gataccggcg cggcggcgat cgaggctttc gccggccaat cgcagaccga
      181 cattttcatc ctttacccgc ataaccgcgt ctccgacgtg cagcgccggc agatgacgac
      241 gatcgatcag cccaacgttc acgcgatcgc cgtcgaaggc tcgttcgacg actgccaggc
      301 gatcgttaaa ggcttgttca acgatctttc cttccgcgac gcgcatcatc tgaccggcgt
      361 caactcgatc aattgggcgc ggatcgtcgc ccagatcgtt tattatttca ccagcgcggt
      421 gacgctcagc gcgccgcatc gcagcgtctc gttcacgacg ccgaccggca atttcggcga
      481 catcctcgcc ggctggtgcg ccaagcggat gggattgccg atcaagagat tggtcatcgc
      541 caccaacgcg aacgacattt tggcgagagc cttggcgagc ggcgaatatc gcgcgcggga
      601 ggtgatcgcg acgcagtcgc cctcgatgga catccaggtc tcgtcgaatt tcgagcggct
      661 gctgttcgac gcctatggcc gcgacgctgc ggccttacgc gggctgatgg cgaatttcgc
      721 acaatcgaaa agcttcgcca ttgcgcccgg cccgctcaag gcgatccgcg acgatttcga
      781 cgctttcgcc accgacgaag agacatgcat cgaggaaatg gcgcggacct atcgcgaggc
      841 cggctatccg ctcgatccgc atagcgcggt cggtgtcaac gcggcgcggc gcgccttggc
      901 ccgcgatccc tcgaccccga tgatcgcgct cgccaccgcc catcccgcca aattcccgga
      961 ggcggtcgcg cgggcgaccg gcgggcgacc ggcgctgccg gaccatctga aaggtttatt
     1021 ggagaagaag gagcgcttcg cgattctgcc gaacgatctt ggcgccgtcc gccaattcat
     1081 ccgcgcccga gcgagggccg ccgcgtgagc gtcgagatta ccaagctcga gtccggcctg
     1141 cgggtcgtca ccgacgcgat gccgcaattg cgcaccgcgt cgctcggcgt ctttgtcggc
     1201 gccgggtcgc gcgacgagcg gccgcaagag catgggcttt cgcatctgct cgaacatatg
     1261 gccttcaagg gcacgcgccg ccgcagcgcc cggcagatcg ccgaggagat cgagaacgcc
     1321 ggcggcgatc tcaacgccga gaccggcgtc gagcagacgg cctatttcgc ccgcgtcctc
     1381 ggcgaggatt gcgagctggc gctcgacatc ctctcggaca tcttgaccga cagccaattc
     1441 gacgcggccg agctcgagcg cgaaaagggc gtgatcctgc aggagatcgg cgcggtcgag
     1501 gacgcgccgg acgatctcgt cttcgatctc ttcaccgaga ccgcctggcc cgaacaggcg
     1561 atcggccggc cgatcctcgg cacgcgcgag cgcgttcgcg ccttcgaccg cgccgcgatc
     1621 gatctctatc tgcgccgtca ttatcaggcc ggcgcgacga tcgtcgccgc ggccggcgcg
     1681 gtcgagcacc gacgcatcgt cgatcttgcc gaacgtctgt tcgcgacgct tggcaagggc
     1741 gattttccag ccgcgccgcc ggcgctttat cgcggcggcg agagcatgac caaaaagggt
     1801 ctcggccaga cccatatcgt tgtcggcttc gaagggcgcg ccgcgcatga cgccgatcac
     1861 gacgccgccc atgtcttcgc cgccgcgacc ggcggcggca tgtcctcgcg cctgtttcag
     1921 gaggtgcgcg aaaagcgcgg cctcgcttat tcgatctacg ctttccactg ggcctattcc
     1981 gattccgggt tgatgggctt ttacgccggc acgtcgcccg ccgacgccgg cgcgctaatg
     2041 gaggcggcgc tcgattgcct tgccgaggcc tcgcattcgc tgagcgagga ggaggtccat
     2101 cgcgccaagg cgcagatgaa ggtctcgctg ctcgccgcgc tcgaatcctc cggcgcgcgg
     2161 gcgcaacaat tggcgcggca catcctgatc tatggccggc cgctcaccgt cgaagagatg
     2221 acggcgcgga tcgatcgcct gaccgtcgcc gatatccgca aggccggcgc ggcgatgctg
     2281 cgctcggcgc cgaccgtgac ggcgatcggc cgcgtcaaaa aggttctcga ccaggaccgc
     2341 gtcgcccgtc gccttggcgg ggtctgaagc cgcgatggcg ctcttccgca tcaaccgcct
     2401 cgctgatccc gcgccgttgg cgcgcggcga cgggctctat ttgcggccgg cgcagagcgt
     2461 cgattacgcg gcctgggcgg ctttgcgcga ggcgagtcgc gccttcctga cgccgtggga
     2521 gccgtcctgg cctgccgacg acctgacccg cgccgccttt cggcgacgat tgaagcggca
     2581 agccgaggag atcgcccgcg acgaggccta ttccttcctg attttcgagg aaggctcgga
     2641 cgatatgctt ggcgggatca cgatcggcgg cgtccggcgc ggcgtcgccc aggccgcgac
     2701 gctcggctat tggatcggcg cgccctatgc gcgacgcggc catatgacga gagccgtcgc
     2761 cgccgcgacc gcttttggct tttcctcgct ccgcctgcat cgcatcgagg cggcctgcat
     2821 tccggacaat gtcgcctcgg cccgcgtgct ggagcgcaac ggtttccgca aggaaggctt
     2881 cgcccgcgcc tatctgcgca tcaacggcgc ctggcgcgat catctcttat ttgctcttct
     2941 cgaaagcgac gcgcgcgcaa ttcagcaagg gggacgcggc gttggttaaa cgctgggcgc
     3001 ggctggcgct gctgtttctg tttctcggcc tgttcagcca ggccgccttc gccgtgcaat
     3061 cggttcgcgt gccgatcgac gccgacgcga tcgatctcgg cagcgcggtc gagcattaca
     3121 gcgcgcaagg cgaccgcatt caggtttcga ccgcgccggg cgcggacggc atcgtccggc
     3181 gtatcgaggt cagcgcgctt gaagccggct cgcaagcctc atggatcgtt ttcgcgctga
     3241 ccaacgacac cgacgagcaa ttgacgcgcc tgatcgtcgc gccgcatttc cggctggtcg
     3301 gatcggggct gatctggccc gatctcggct cgagccggat cacgacgatc accgcgagcc
     3361 agggcgaagc gccggagcgc gaggaccatc ccgacgccga cgtgtttcgc ctgacgctcg
     3421 atccgggcgc caccgtcacc tatgtcgccg aattgcgcac gcccaatctg ccgcaaatct
     3481 acttgtggga gcccgacgcc tataaggaca aggtggcgag cctgacattg taccagggca
     3541 tcgtcatcgg catagccggc ctgctcgctc tgttcctgac catcgtcttc gtcgtgcgcg
     3601 gcgcggtcat cttccccgcc gccgcggcct tggcctggac cgtcctcgcc tatgtctgca
     3661 tcgatttcgg cttttggcgg aagattttcg gctttgagga cgaggccgaa cgaatctggc
     3721 gcgccggcgt cgaggcgacg ctcgccgcaa ccctgatcat cttcctcttc gcctatctca
     3781 acctgcgccg ctggcatgtg cgctattcgc atatcgcggc gatctggctg ttcctgatgg
     3841 cggcggtgat cggcctttcg gtctataatg cgccgatcgc cgccggcgtc gcgcgcatct
     3901 ccatcgccac cgcggcgggc atcggcttca tcctcatcct caatatggcg gcgcgcggct
     3961 ccgaccgcgc ggtgatgctg atccccgcct ggttcctgct gctcgtctgg gtcgtcggcg
     4021 ccggcttcac cgtcaccggc gcgctgacca acgacctcgc agcgccgggc ctgatcggcg
     4081 gcctggtgct gatcgtgatg ctgatcggct tcacggtgat gcagaacgcc ttcgccggct
     4141 ccggcctttc cagcatcaga ggcccggatt cggagcgccg ggcgctcgcc atgaccggct
     4201 cgggcgacgc gattttcgat tggagcgtcg aatccgacca gatctatgtc agcccggaaa
     4261 tcgaaaacca gctcggcttg ccgcgcggcg cgctgcaagg cccggcgacg tcctggctcg
     4321 acatgctcca tccgatggac cgcgaccgct acgccgcggt gctggacggg ctgctgcagc
     4381 agcgctgcgg gcgggtcaat cacgacctgc gcttgcgcgc cgccgacggc caatatttct
     4441 ggtacgtgct gaaggcgcgg ccggtggtca acgccgatgg cgaggtgatg cgcgtcatcg
     4501 gctcgctcgc cgacgtgacc gagatgaaga ccgccgagga gcgcatgctt cacgatgcgg
     4561 tccacgacaa tctgaccggc ctgcccaatc gcgaattgtt cttcgaccgg ctcgaaagcg
     4621 cgctgattgc ggcgaaacgg ccgggcggtc cgcggccggc ggtgatggcg atcgatgtcg
     4681 accgcttcaa gcagatcaac gaggcgatcg gcctctccta tggcgattcg gtcctgctca
     4741 ccgtggcgcg gcgcctgggt cgaaacttaa ggcccggcga caccttggcg agaatcgccg
     4801 gcgatcagtt cggcgccatc gtgatgaccg aggacgaagc cgacaatatt ctcgattttg
     4861 tcgacacgac gcgcaacatc ctgtcgacgc cgatcagttt cggcgatcgc gaagtggcgc
     4921 tgaccgtgtc gatcggcgtc gccctgttcg atccgaacgc ccacgccaag ggcggcgaca
     4981 ttttgaagga cgcggaaatc gccctcgccc acgccaagaa atccggcggc gaccgcgccg
     5041 agc
//
LOCUS       DATGJW010000268         2808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_776300, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000268 DATGJW010000000
VERSION     DATGJW010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2808
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_776300"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            65..1270
                     /locus_tag="VKU03_08340"
     CDS             65..1270
                     /locus_tag="VKU03_08340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ71311.1"
                     /translation="MKSLSAALCALTLIASAAASAPAAAEDTIKIGEINSYSALPAFT
                     EPYRKGWQLALEEINAAGGVDGKKLEVISKDDGGKPAEAVTAANELVAKDGVVMLAGT
                     FFSNIGLAVSDFAKQKKILFLAAEPLTDAITWSKGNRYTFRLRPANYEQAGMLAEEAA
                     KLPAKKWATIAPNYEYGQSAVAAFKKLLSAKRPDIQWVGEQWPPQGKIDAGAVVEAIA
                     KTNPDAILNVEFGADLVKLVREGETRGLFKGRSVVSFLTGEPEYLDPLKDETPQGWIV
                     TGYPWNNVSIPEHAAFLKAYEAKYHDYPRLGSVVGYSTLKAAAAILAKAKSTDSEKLV
                     DAAEGIEVETPFGKITFRKADHQSTLGAFVGKTALKDGKGVMVDAEYRDGAKYLPPES
                     EVKALRPQD"
     gene            1498..>2808
                     /locus_tag="VKU03_08345"
     CDS             1498..>2808
                     /locus_tag="VKU03_08345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71312.1"
                     /translation="MTFFFVQFVTGLSSASSLFLAACGLSIIFGVTRVVNFAHGAFYM
                     LGAYIGFTLTARLSGAFGFWGGIVLASLVVAAIGAAIEMALLRRLYSGPELLQLLATF
                     GVTLAVEDIALLIWGPEDLLGRRAPDFKGAVNLFGQAFPTYDLLLIALGPLVLVLLWL
                     LFSQTRWGVLVRAATQDREMVAALGVNQKWLFTSVFALGVFLAALGGALELPREAVSH
                     TMDLQIITEVLVVVVIGGLGNMLGTFLAAIIVSELNAFGILVLPKISIVLVFLIMALV
                     LIVRPWGLLGRAEAAPRLAGSAPAAPWRPLDARGRILALLALVAAALLPLVAGDYGLA
                     VATEVLIFVLYAASLHALVGAGGLASFGHAAYFGFGAYAAALALRGLSLPMEAALVAG
                     PLLGLAGALIFGWFSVRLSGVYFAMLTLAFAQIAWSVAFQWTEVT"
BASE COUNT          434 a          915 c          903 g          556 t
ORIGIN      
        1 aatgacacgg cgcttgggct tggctgatgc ggcgcgacta aggtactttt gggaggagtg
       61 aatcatgaaa agcctatccg ccgcgctctg cgcgctgacc ctgatcgcat cggccgcggc
      121 aagcgcgccg gctgcggccg aggacacgat caaaatcggc gagatcaatt cttattccgc
      181 tctgccggcc ttcactgaac cttatcgcaa aggctggcaa ttggcgctcg aggagatcaa
      241 cgcggccggc ggggtcgacg gcaagaagct cgaagtgatc tccaaggacg acggcggcaa
      301 gccggccgag gcggtgaccg cggccaacga gcttgtcgcg aaggacggcg tcgtcatgct
      361 ggccgggacg ttcttctcca atatcggcct cgcggtctcg gatttcgcca agcagaagaa
      421 gattctcttc ctcgccgccg agccgctgac cgacgccatc acctggtcga agggcaaccg
      481 ctatacgttc cgcttgcggc cggccaatta cgagcaggcg ggcatgctgg ccgaagaagc
      541 cgccaagctg ccggccaaga aatgggcgac gatcgcgccg aattatgaat atggccaatc
      601 ggcggtcgcg gcgttcaaga agctgctttc ggcgaagcgg ccggacattc aatgggtcgg
      661 cgagcaatgg ccgccgcaag gcaagatcga cgccggcgcc gtggtcgagg cgatcgccaa
      721 gaccaatccc gatgcgatcc tcaatgtcga gttcggcgcc gatctcgtca agctggtccg
      781 cgaaggcgag acgcgcgggc tgttcaaagg ccgttccgtg gtcagcttcc tcactggcga
      841 gccggaatat ctcgatcctt taaaggacga gacgccgcaa ggctggatcg tcaccggcta
      901 tccttggaat aacgtctcca ttcccgagca cgccgctttt ctgaaggcgt acgaagcgaa
      961 ataccacgat tacccgcgac tcggctcggt ggtcggctat tcgaccttaa aagcggccgc
     1021 agcgatcctc gctaaggcca agtcgaccga cagcgagaag ctggtcgacg cggccgaggg
     1081 aatcgaggtc gagacgcctt tcggcaagat cactttccgc aaggccgacc atcaatcgac
     1141 cctcggggcc tttgtcggca agacggcgct gaaggacggc aagggcgtga tggtcgacgc
     1201 cgaataccgc gacggcgcga aatacctgcc gcccgagagc gaggtgaagg cgctcaggcc
     1261 ccaggattga gcgtcggtgt gctctgacgc gcgcctatcc tcccccgcga agcgggggag
     1321 ggggaccatg cgaagcatgg tggagggggc gcaggcggct caggataaga ctccaaactc
     1381 cgaggccccc tccaccggcc ttcggccggt ccccctcccc cgttgcgctt cgctccacgg
     1441 gggaggaaac gggcgcccta tccttccccg cttcgctggg gaggaaggta cgccatagtg
     1501 accttcttct tcgttcaatt cgtcacgggc ctttccagcg cctcgtcgct gtttctcgcg
     1561 gcttgcggcc tgtcgatcat tttcggcgtg acgcgcgtcg tcaatttcgc ccatggcgcc
     1621 ttctatatgc tcggcgccta tatcggcttc acgctgaccg cgcgtttgtc gggcgctttc
     1681 ggcttctggg gcgggatcgt cctggcttcg ctcgtcgtcg cggcgatcgg cgccgccatt
     1741 gagatggcgc tgctgcgccg cctctacagc gggcccgaat tgctgcaact gttggcgaca
     1801 ttcggcgtga cgctcgccgt cgaggacatc gccctgctga tctgggggcc ggaggacctg
     1861 ctcgggcggc gcgcgccgga ttttaagggc gcagtcaatc ttttcggcca agcctttccc
     1921 acttacgatc tcctgctcat cgcgctcggg ccattggtgc tggtcctgct ctggcttctc
     1981 ttcagccaga cgcgctgggg cgtgctggtg cgcgcggcga cgcaggaccg cgaaatggtc
     2041 gccgcgctcg gcgtcaacca gaaatggctc ttcaccagcg tcttcgcgct cggcgttttt
     2101 ctcgccgcgc tcggcggcgc gctggagctg ccgcgcgaag cggtgagtca tacgatggat
     2161 ttgcagatca tcaccgaagt tctggtcgtg gtggtgatcg gcgggctcgg caatatgctc
     2221 ggcacgtttc tcgccgcgat catcgtctcc gagctcaacg cgttcggcat tctcgtcttg
     2281 ccgaagattt ccatcgttct ggtttttctg atcatggccc tcgtgctgat cgtcaggcct
     2341 tggggcttgc ttggccgcgc ggaagccgcg ccgcggctcg ccggctccgc cccggcggcg
     2401 ccatggcggc cgctcgacgc gcgcggccgg atcctcgcgc tcctggcgct cgtcgctgcg
     2461 gcgctgcttc ctttggtcgc cggcgattac ggcctcgcgg tcgcgacgga agtgctgatc
     2521 ttcgtactct atgcggcgag cctgcatgcg ctggtcggcg ccggcggcct ggcgtcgttc
     2581 ggccacgccg cctatttcgg cttcggcgcc tatgccgccg ccctcgcctt gagggggctg
     2641 agcctgccga tggaggccgc cttggtcgcc ggccctttgc tcggcctcgc cggcgcgctc
     2701 atcttcggct ggttctccgt gcgcctttcc ggcgtctatt tcgccatgct tacgctcgcc
     2761 ttcgcccaga tcgcctggtc cgtcgctttc caatggaccg aggtcacc
//
LOCUS       DATGJW010000269         2848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1295296, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000269 DATGJW010000000
VERSION     DATGJW010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2848
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1295296"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..708)
                     /locus_tag="VKU03_08350"
     CDS             complement(<1..708)
                     /locus_tag="VKU03_08350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019962635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citramalate synthase"
                     /protein_id="HLJ71313.1"
                     /translation="MSRERLYLFDTTLRDGAQTTGVDFSLEDKRQIAALLDRLGVDYV
                     EGGYPGANPLDTKFFEKKATERARFAAFGMTKRPGRSAENDPGIAALLAAKADAIVFV
                     AKSWDYHVRVALGATLEENLDSISSSVKAAVEAGREAMVDCEHFFDGYKANPDYALAC
                     ARTAFESGARWVVLCDTNGGTLPDEVARIVGEVARVVPGERLGIHAHNDTENAVANSL
                     AAIGAGARQIQGALNGLG"
     gene            complement(705..1235)
                     /locus_tag="VKU03_08355"
     CDS             complement(705..1235)
                     /locus_tag="VKU03_08355"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ71314.1"
                     /translation="MSADRAPPASLRPYLPSDAALCAAIFRAAVKELTADDYDEDQRE
                     AWASGSDDVEAFGARLAGALTLVALAEGGPVGFASLKGDAIDMLFVAPAVAGRGVGTM
                     LLDALIKLAGARGLAKLTSDVSDTARRLFEKQGFVGQQRNIVRKGEVWLANTTMTKTL
                     AGAAAPAGARHQGRGR"
     gene            complement(1232..2629)
                     /gene="cysS"
                     /locus_tag="VKU03_08360"
     CDS             complement(1232..2629)
                     /gene="cysS"
                     /locus_tag="VKU03_08360"
                     /EC_number="6.1.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004817 - cysteine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006423 - cysteinyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine--tRNA ligase"
                     /protein_id="HLJ71315.1"
                     /translation="MSEPLRLYNTLTRSKEVFKPIDPNHVRLYVCGPTVYDYAHIGNA
                     RPLIVFDVLFRLLRHRYGDDHVVYVRNITDVDDKINARAAEEYPDLTLNEAIRKVTDK
                     TDRQFHEDAAALGCLPPTVEPRATEHIAEMRALIERLIASGHAYVAEDNVLFSVASMP
                     DYGRLSKRPFDEMIAGARVDVAPYKRDAMDFVLWKPSKSGEPAWPSPAGIAAPGRPGW
                     HIECSAMSWRHLGEIFDIHGGGVDLVFPHHENEIAQSRCAFHTPVMANYWMHNGFLQV
                     EGEKMSKSLGNFVTIRDALADWPGEVIRLNMLKTHYRQPIDWTITALRESQRELDRWY
                     PLAKQYAASSAMLQPDFIDAINDDLNTPDAVTALRRLYKEAMDGVGLAGARLVSCGHF
                     LGLFQQEIAAWLHNKPTALTVDEIRVTRLIKSRAEARAAKNWPESDRIRDELAAMGVA
                     LKDNKDGTTSWEVKR"
BASE COUNT          502 a          905 c          921 g          520 t
ORIGIN      
        1 gccgagcccg ttcaacgcgc cctggatttg ccgcgcgccg gcgccgatcg cggcaagcga
       61 attggcgacg gcgttttcgg tgtcgttatg ggcgtgaatg ccgagccgct cgcccggcac
      121 gacgcgcgcc acctcgccga cgatgcgcgc gacctcgtcc ggcaatgtgc cgccgttggt
      181 gtcgcaaagc acgacccagc gcgcgccgga ctcgaaagcc gtgcgggcgc aggccagcgc
      241 gtaatcggga ttggctttgt agccgtcgaa gaaatgctcg caatcgacca tcgcctcgcg
      301 gccggcctcg accgccgcct tgacgctcga tgaaatgctg tcgagatttt cctcgagcgt
      361 cgcgccgagc gcgacgcgga cgtgatagtc ccagctcttg gcgacgaaga cgatcgcgtc
      421 ggccttggcg gcgagcaagg cggcgatgcc gggatcgttc tcggccgaac ggcccggcct
      481 tttcgtcatg ccgaacgcgg cgaagcgggc gcgctcggtc gccttcttct cgaagaattt
      541 ggtgtcgagg ggattggcgc cgggatagcc gccctcaaca tagtcgacgc cgagccggtc
      601 gagcagagcg gcgatctggc gcttgtcctc gagcgagaaa tcgacgccgg tcgtctgcgc
      661 gccgtcgcgc aaggttgtgt cgaacagata gaggcgctct cggctcatcg gccccggccc
      721 tgatgtctcg cgccagcggg cgccgcggcg ccggcgagcg tcttggtcat cgtcgtgttg
      781 gcgagccaga cttcgccctt gcgcactatg ttgcgctgct ggccgacaaa accctgcttc
      841 tcgaacagcc gccgcgccgt gtcgctgacg tcgctggtca gcttggcgag gccgcgcgcc
      901 ccggccagct tgatcagcgc gtcgagcagc atcgtcccga cgccgcggcc ggcgacggcc
      961 ggggcgacga acagcatgtc gatcgcgtcg cccttgaggc tggcgaagcc gaccggcccg
     1021 ccctcggcaa gggcgaccaa ggtcagcgcg ccggcgagcc gcgcgccgaa ggcttcgacg
     1081 tcgtcgctcc ccgacgccca ggcctcgcgc tgatcctcgt cgtaatcgtc ggcggtcagc
     1141 tccttgaccg ccgcgcggaa gatcgccgcg cagagcgcgg cgtcggaggg gaggtacggg
     1201 cgaaggctgg ccggcggcgc gcgatcggcg ctcatcgctt gacctcccaa cttgtcgtgc
     1261 cgtccttgtt atccttgagc gcgacgccca tcgcggcgag ttcgtcgcgg atgcggtcgg
     1321 attcgggcca gtttttcgcc gcccgcgcct cagcgcgcga tttgatgagg cgggtcacgc
     1381 gaatctcgtc caccgtcaaa gctgtcggct tgttatgcag ccaggccgcg atttcttgct
     1441 gaaacagacc gaggaaatga ccgcaagaga caagcctggc gccggcgaga ccgacgccgt
     1501 ccatcgcttc tttgtagagc cggcgaagag ccgtaaccgc atcgggcgtg ttcaagtcgt
     1561 cgtttatcgc atcgatgaag tccggctgga gcatcgccga ggacgcggca tattgcttgg
     1621 cgagcgggta ccatcggtcg agctcgcgct gcgactcgcg caacgccgtt atcgtccaat
     1681 cgatcggctg ccggtaatga gtcttgagca tgttgagtcg gatgacttct cccggccaat
     1741 ccgccagcgc gtcgcggatc gtcacgaaat tccccaggct tttcgacatt ttctcgcctt
     1801 cgacctgaag aaagccgtta tgcatccagt aattcgccat gacaggcgta tgaaaggcgc
     1861 agcgcgattg cgcgatctcg ttttcatggt gtgggaagac gagatcgacg ccgccgccat
     1921 gaatgtcgaa tatctcgccg agatgcctcc aactcatcgc ggagcattcg atgtgccagc
     1981 ccggacggcc gggggccgcg attccagcgg gcgacggcca agccggctcg ccggacttcg
     2041 acggcttcca cagcacgaaa tccatcgcat cgcgcttgta cggcgcgaca tcgacgcgcg
     2101 cgccggcgat catctcgtcg aacggacgct tcgacagcct gccataatcg ggcattgacg
     2161 cgacgctgaa taggacattg tcctcggcga cataggcgtg gccggacgcg ataagccgct
     2221 cgatcagcgc ccgcatttcg gcaatgtgct cggtcgcgcg cggctcgacg gtcggcggca
     2281 ggcagccaag cgccgctgca tcttcatgaa actgccgatc ggttttgtcg gtgaccttgc
     2341 ggatcgcttc gttcagagtc aggtccggat attcctccgc agcgcgcgca ttgatcttgt
     2401 cgtcaacgtc ggtgatgttg cggacataaa cgacgtggtc atcgccatag cgatggcgca
     2461 agagacggaa cagcacgtcg aagacgatca gcggccgcgc gttgccgata tgggcatagt
     2521 cgtagaccgt cggcccgcag acatagaggc gcacgtggtt tgggtcgatc ggcttgaaaa
     2581 cctctttcga ccgggtcagc gtattataga gccgcaaagg ttccgacatg tccgttccga
     2641 tagccacgcg tcaaagcgcc tggccggcat tttcgtcgct tttcggatcg aggatgaaaa
     2701 aagacgggcg ccagccagag tcagactagc ggcgaataat aataataatc cggcaaatgc
     2761 tggattgtcc cgacatgatc tttctttccc gcaggcggca aaggaagtca aggcgcatgt
     2821 cattcccgcg aaaagcggga atccagac
//
LOCUS       DATGJW010000270         2499 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_50765, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000270 DATGJW010000000
VERSION     DATGJW010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2499)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2499)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2499
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50765"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            57..1151
                     /locus_tag="VKU03_08365"
     CDS             57..1151
                     /locus_tag="VKU03_08365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016847625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase family
                     protein"
                     /protein_id="HLJ71316.1"
                     /translation="MKAWRALGNGRLELQDLPAPRPAAGGVVLRMQAASMLSYLRQVL
                     DGSLGYDTPPRPFTPGTNGIGVVEAVGAGVYHLAPGQRVTLDPHLVANEPVAEPAQIL
                     LGLTGMRASETGRPHGPTKALQASWPDGTYAEMVHMPAAVATPLPAALDAMPAAKLAA
                     IAKFAVPYGGLLRMNLQAGETIVVNGATGYFGGAGALLALALGAGRVVAAGRDAKALD
                     DLAGAAGPRLVPVALSGEVGRDVDALRAAAGGGADCALDLVGRADSANATLAALRSLR
                     RGGRLALMGSVRQPLPLTVGEMLGQDWTVMGVFMYPRDAPARLARLIAAGQLDLGLIR
                     ERAFPLADIEAAMDAAAKMRSRDLTVLTMD"
     gene            complement(1273..>2499)
                     /gene="hflX"
                     /locus_tag="VKU03_08370"
     CDS             complement(1273..>2499)
                     /gene="hflX"
                     /locus_tag="VKU03_08370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HLJ71317.1"
                     /translation="EARFDEAIGLAGAIDLTIAGAELVNLNEIRPSTFLGKGKVDEIA
                     GLVKSFDIGVVVMDCALSPVQQRNLEKAFSAKVIDRTGLILEIFGRRARTREGALQVE
                     LAHLAYQKSRLVRSWTHLERQRGGFGFLGGPGETQIETDRRLIQERMIRIERELDSVK
                     RTRALHRASRSEVPYPVVALVGYTNAGKSTLFNRLTRSSVVAEDMLFATLDPTMRALE
                     LPHGGTAILSDTVGFISDLPTMLISAFRATLEEVLAADVILHVRDISHADSDAQSADV
                     AAILAELGVSPSAGRVIEVWNKLDKLDGDRREALRGVRNGEAKSEAAIAVSALTGQGL
                     DALLAAIEDRLAAGRALVDLDIDSADGQGLHWLYENTEVMARRQAEDGKIHLRLRVTP
                     DKMGRVARRFPNIAQL"
BASE COUNT          366 a          855 c          878 g          400 t
ORIGIN      
        1 gagacttcaa gtctcgcaat ataatgagac tccgagttcc ggaattggag gcgacgatga
       61 aagcttggcg cgcgctggga aacggccgat tggaactgca ggacctgccg gcgccgcggc
      121 cggccgcggg cggcgtggtg ctgcgcatgc aggccgcctc gatgctctcc tatctgcgcc
      181 aggtcctgga cggctcgctc ggctacgaca cgccgccgcg gccgttcacg ccggggacaa
      241 acgggatcgg cgtcgtcgag gccgtcggcg ccggcgtcta ccacctcgct ccggggcagc
      301 gagtcacgct cgatccgcat ctggtcgcga acgagccggt cgccgagccg gcgcagattc
      361 tgctcggcct gaccggcatg cgcgcgtccg agacgggccg cccgcatggg ccgacaaagg
      421 ctttgcaggc ctcctggccg gacgggacct atgccgagat ggtccacatg cccgccgcgg
      481 ttgcgacgcc gctgcccgcc gcgctcgacg ccatgccggc ggcgaagctc gcggcgatcg
      541 ccaaattcgc cgtgccctat ggcggtctgt tgcggatgaa cctgcaagcc ggcgagacga
      601 tcgtcgtcaa tggcgcgacc ggctatttcg gcggcgccgg ggcgctgctg gcgctggcct
      661 tgggggccgg ccgggtcgtc gccgccgggc gcgacgccaa ggcgctcgac gatctcgccg
      721 gcgcggcggg cccccgcctc gttccggtcg ccctgagcgg cgaggtcggc cgcgatgtcg
      781 acgcgctgcg ggccgccgcc ggcggcggcg ccgattgcgc gctcgatctc gtcggccgcg
      841 ccgacagcgc caatgcgacg ctggcggcct tgcgcagcct caggcgcggc ggccgcctcg
      901 ccctgatggg cagcgtgcgc cagccgctgc cgctgaccgt cggcgaaatg ctgggccagg
      961 attggacggt gatgggcgtc ttcatgtatc cgcgcgacgc tccggcgcgg cttgcccggc
     1021 tgatcgccgc cggccagctc gatctcggcc tgatccgcga gcgcgccttt ccgctcgccg
     1081 acatcgaagc ggcgatggac gccgccgcca agatgcgcag ccgcgacctc accgttttaa
     1141 cgatggactg aaggttgctt aagtctgagc gatggtaacc gggggcaaat ttccgggcgg
     1201 ggattaggcg cggtcgtcga ccgcgccgcg cgaaacgggg cgcttcgcgc ccgccgcggt
     1261 cagcgaccgc ggctacaact gggcgatgtt cggaaaccgg cgcgccaccc ggcccatctt
     1321 gtcgggggtg acgcgcagcc gaagatggat ttttccgtcc tcggcctgcc ggcgggccat
     1381 cacttccgtg ttttcgtaaa gccagtgcag cccttgcccg tcggcgctgt cgatatcgag
     1441 gtcgaccagc gcgcggccgg cggccagacg atcctcgatc gcggcgagca gcgcatccag
     1501 cccttggccg gtcaaagccg acaccgcgat cgccgcctcg cttttggcct cgccgttgcg
     1561 gacgccgcgc agcgcctcgc ggcggtcgcc atcgagcttg tccagcttgt tccagacttc
     1621 gatcactcgc ccggcgctcg gcgatacgcc aagctcggcg aggatcgccg ccacatccgc
     1681 gctctgcgca tcgctgtcgg catgcgagat gtcgcggaca tgcaggatga cgtcggccgc
     1741 cagcacttcc tccagcgtcg cgcggaaggc ggaaatcagc atggtcggca ggtcggagat
     1801 gaagccgacc gtgtcggaga ggatcgcggt cccgccatgc ggcagttcga gcgcgcgcat
     1861 ggtcggatcg agcgtcgcga acagcatgtc ctcggcgacg acgctcgatc gggtcagccg
     1921 gttgaagagg gtcgatttgc cggcattggt gtagccgacc agggcgacca ccggataggg
     1981 aacctcgctg cggctcgcgc gatgcaaggc gcgcgtgcgc ttgacgctgt cgagttcgcg
     2041 ctcgatgcgg atcatccgct cctggatcag gcggcggtcg gtctcgatct gagtttcgcc
     2101 ggggccgccg agaaagccga agccgccgcg ctggcgctcc aaatgggtcc aggaccgcac
     2161 caggcgcgac ttctggtagg cgagatgggc gagttcgacc tgcagcgcgc cctcgcgcgt
     2221 gcgcgcccgg cggccgaaaa tttcgaggat caggcccgtc cggtcgatca ccttggccga
     2281 gaaggccttt tccaggttgc gttgctgcac cggggacagc gcgcaatcca tcacgacgac
     2341 gccaatatcg aagcttttga cgagaccggc gatttcatcg accttgcctt tgccgagaaa
     2401 ggtcgacggc cggatttcgt tgaggttgac gagctcggcg ccggcgatcg tcagatcgat
     2461 cgcgccggca aggccgatcg cctcgtcaaa gcgcgcctc
//
LOCUS       DATGJW010000271         7436 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1087737, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000271 DATGJW010000000
VERSION     DATGJW010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7436)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7436)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7436
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1087737"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            503..2299
                     /locus_tag="VKU03_08375"
     CDS             503..2299
                     /locus_tag="VKU03_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339213.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ71318.1"
                     /translation="MPPLLQLRDIRLSFGATPLLQGAELSVAPGERVALVGRNGSGKS
                     TLLKIAAGAIEADAGERFVQPSAALRYLPQEPDLKGFATALDYVLAGLDESGDSSRAR
                     ALLGALGLAAEADPRTMSGGQARRAALARVLAPSPDILLLDEPTNHLDIAAIEWLEGE
                     LKASRAALVVISHDKRLLANLTDATVWLDRGATRRLDRGFAAFEAWRDKLLDEEEAER
                     HKLDRRIAAEEHWLRYGVTARRKRNQRRVGELRALRVERREAKRVQGDVKLIASDAPL
                     SGKLAIEAERLSKAYDGQPIVEDFSIRVLRGDRLGVVGPNGAGKTTLVNLLTGALAPD
                     SGTLRLGANLAMASLDQSRASLDPETTLSDALTGGGSDYVSVNSERKHVVGYMRDFLF
                     SPDQARTPIGRLSGGERGRLMLARALAQPANLLALDEPTNDLDMETLDLLQEMLADYA
                     GAILLVSHDRDFLDRVATSVVVNEGEGRWVEYAGGYSDMLAQRGLAPAPAPERPVKPA
                     KTEPPAPRPAGKRRMSFKDKHALETLPARIDGLRAELAALDAKLADPDFHARDAAGFA
                     RASSRYAEARDELSAAEDEWLSLEILREEIEG"
     gene            2361..3539
                     /gene="uxuA"
                     /locus_tag="VKU03_08380"
     CDS             2361..3539
                     /gene="uxuA"
                     /locus_tag="VKU03_08380"
                     /EC_number="4.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008927 - mannonate dehydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannonate dehydratase"
                     /protein_id="HLJ71319.1"
                     /translation="MRQAWRWYGPHDNVSLDDVRQAGASEIVSALHAIPPGGEWTREA
                     VAERKRIVEETPFGRTPLKWTIVESIPVPDDVKRFGKAAKAAIDVWIASLEAVAANGI
                     TTVCYNFMPVLDWTRTDLDWLLPNGAKALRFDAARLAAYDIHILKRPGAAQDYAPDVR
                     ERARRTFEKLSQGEAERLIANIGAGLPGTTSEPVDAAGMREKLAQYGKIDAAQLRRHL
                     VEFLERVAPAAEALGVKLTLHPDDPPRPLLGLPRVASNAEDYQALFDAVPTPANGMCF
                     CTGSLGASPANDLPAIARRFAPRIYFAHLRAVKREPDGSFYEADHLDGDCDMVAVLRV
                     LLSEDRRRGSEEKIPFRPDHGHIMLDDLRKKTNPGYSAIGRLKGLAELRGVIRALESL
                     "
     gene            complement(3597..4088)
                     /locus_tag="VKU03_08385"
     CDS             complement(3597..4088)
                     /locus_tag="VKU03_08385"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HLJ71320.1"
                     /translation="MTDETLTQDEAAPRAVNGQAVDLSKLTDLIGYQLRRAQIAVFDD
                     FIRSFAPYDIRPTQYGVLTAIESNPGSSQAAIAQSLGIKRSNFVKLIDEFERRKLVVR
                     KEVAGDRRANALFLTKTGQAELAGLHKVRIAHEARIAAAIGDESDRRALLGQLSQLAA
                     LKG"
     gene            4257..4868
                     /locus_tag="VKU03_08390"
     CDS             4257..4868
                     /locus_tag="VKU03_08390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173797.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031966 - mitochondrial membrane
                     [Evidence IEA];
                     GO_process: GO:0033617 - mitochondrial cytochrome c
                     oxidase assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HLJ71321.1"
                     /translation="MSSPAPSSPPMLPGGRRVFIVIVSAALVLAASLAYVLTSVRAGA
                     PAASLIGGPFKLVAGDGRTITDADMKGEPFLVFFGYTHCPDVCPATLAEISDVLAKMP
                     GKPVKALFITIDPERDSAAVMKDYVSNFDPRIIGLTGDRAAIDQVEKAYRVYARKAPQ
                     AGGDYTMDHSAIVYLMDSQGRFVEAFNLERKPEDSAKELEKYL"
     gene            complement(4923..6716)
                     /gene="mutL"
                     /locus_tag="VKU03_08395"
     CDS             complement(4923..6716)
                     /gene="mutL"
                     /locus_tag="VKU03_08395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0032300 - mismatch repair complex
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HLJ71322.1"
                     /translation="MPIRRLDPLLIDRIAAGEVIERPAAAVKELVENALDAGATRIDV
                     AIEAGGRSLIRVIDNGSGMGAADLALCVERHATSKIPDGDLSAIATFGFRGEALPSIA
                     SVSRLELRSRAAGATEAFLIRIEDGVKGEVQPCAQPQGTRIEARDLFAATPARLKFLK
                     SERAESQAAGDVVRRLALANPAVRFSFASEASQGFDWPACAPDEEGRAARTRQALGAE
                     FSDNALAIAAEREGVKLSGFAGLPTYHRANALQQYFFVNGRTVRDKLLAGALRAAYAD
                     YLPRERHPAVALFIECDPREVDVNVHPAKAEVRFRDPGLVRGLIVSALKAALQAALHR
                     AATTGGDGAIAALRAPNGFSGSPRPANGGWDWRASPAAPLGFAEPAQQAFDGFAPAAA
                     IKAEPVEAAADLAAPLGAARAQVHDSYVIAQTADGVVIVDQHAAHERIVYERLKRQRA
                     ESGVARQILLTPFVADLDPDAAALVAERAESLRELGLIVEGFGPGAVLVREVPALIDG
                     ADIGALISDLADELAAEDGALSLERRLDHRLATIACHHSVRAGRRLKPEEMNALLREM
                     ERTPGAGQCNHGRPTYVELKLADIERLFGRR"
     gene            complement(6766..6906)
                     /locus_tag="VKU03_08400"
     CDS             complement(6766..6906)
                     /locus_tag="VKU03_08400"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLJ71323.1"
                     /translation="MTSKAWIAPGDALHLAIAANHGATIVTLDRGLAEAAAELAVPAR
                     LL"
BASE COUNT         1187 a         2526 c         2508 g         1215 t
ORIGIN      
        1 tgcttggaat agtcgaagag cagatcgtcg agcacgacat gcatgctctc gaaacgctcc
       61 ggatcggcgg cgaactcgtc ggcgacgctc cgcgccagga cctgccggct cagcgcttgc
      121 agagcgctct tgcgcgcggg atcgatcata aaggctccca gtgggctgtc gcctcggcgg
      181 cagcgacaac gagtattcaa aggcggcggc ttaccctcgc cccatccctc tcccgcacga
      241 gagagggcgc ctcgcggctt ccatcagccg tcgcgctata ggcgcggcca acgaccgcgg
      301 ctacaacccc ttctcccgcc tgcgggagaa gggggcgcga agcgccggat gagggcatgc
      361 cgcgacgcag acgaacggcg tccaagtcac tgagcttacc cgaaaagacg gcggaattta
      421 agcccacggc ggcggggaaa tccgctttct ctttccggcg cgccgctcta agggtgcggc
      481 ccccaacgct ttttgttcga tcatgccgcc tctgctgcaa ttgcgggaca ttcgcctgag
      541 cttcggcgcg acgcctttgc tgcaaggcgc cgagctcagc gtcgcgcccg gcgagcgcgt
      601 cgccttggtc gggcgcaacg gctccggcaa gtcgaccttg ctcaaaatcg ccgcgggcgc
      661 gatcgaggcg gatgccggcg agcgcttcgt tcagccaagc gccgccttgc gttacttgcc
      721 gcaggagccg gatctcaaag gcttcgcaac ggcgctggat tacgttctcg ccggcttgga
      781 cgagagcggg gattcgagcc gcgcccgcgc cttgctcggc gccctcggcc tcgccgccga
      841 agccgatccg cggacgatgt cgggcggcca ggcgcggcgc gccgctttgg cccgcgttct
      901 ggcgccctcg cccgacatcc tgctgctcga cgagccgacc aaccatctcg acatcgccgc
      961 gatcgagtgg ctggagggcg agctcaaggc ctcgcgcgcg gcgctggtcg tgatcagcca
     1021 cgacaagagg ctgctcgcca atttgaccga tgcgacggtc tggctcgatc gcggcgcgac
     1081 gcggcggctc gaccgcggct tcgccgcgtt cgaggcttgg cgcgacaagc tgctcgacga
     1141 agaggaggcc gagcgccaca agctcgaccg ccgcatcgcg gccgaggagc attggctgcg
     1201 ctacggcgtc accgcccggc gcaagcgcaa tcagcgccgc gtcggcgagc ttcgcgcctt
     1261 gcgcgtcgag cgccgcgagg ccaaacgcgt ccaaggcgat gtcaagctta tcgcgtccga
     1321 cgcgccgctc tccggcaagc tggcgatcga ggcggagcgc ctgagcaagg cctatgacgg
     1381 gcagccgatc gtcgaggact tctcgatccg cgtgctgcgc ggcgaccggc tcggcgtggt
     1441 cgggccgaac ggcgccggca agaccactct ggtcaatctc ctcaccggcg cgctcgcgcc
     1501 cgattccgga acgctgcggc tcggggccaa tctcgccatg gcgagtctcg accaaagccg
     1561 cgccagcctc gatcccgaga cgacgctctc ggacgccttg accggcggcg gcagcgacta
     1621 tgtctcggtc aatagcgagc gcaagcatgt cgtcggctat atgcgggatt tcctgttctc
     1681 gccggatcag gcgcgcacgc cgatcggccg cctgtcgggc ggcgagcgcg gccggctgat
     1741 gctggcgcgg gcgctcgcgc agcccgccaa tctcctggcg ctggacgagc cgaccaacga
     1801 tctcgacatg gagacgctcg acctattgca ggaaatgctc gccgattacg ccggcgccat
     1861 cctcctcgtc agccatgacc gcgacttcct cgatcgcgtc gcgacctcgg tcgtcgtcaa
     1921 cgaaggcgaa ggccgctggg tcgaatatgc tggcggctat tccgacatgc tcgcccagcg
     1981 cggtctcgcg cctgctccgg cgcctgaacg gcccgtcaag ccggccaaaa ccgagccgcc
     2041 ggcgccgcgg ccggccggca agcgccggat gtcgttcaag gacaagcacg ctttggagac
     2101 tttgccggcg cgcatcgacg ggctgcgcgc cgagctcgcg gcgcttgacg cgaaactcgc
     2161 cgaccccgat tttcacgcgc gcgacgcggc gggcttcgcg cgagcctcgt cgcgctatgc
     2221 cgaggcgagg gacgaattat cggcggccga ggacgaatgg ctgagcctcg aaatcttgcg
     2281 ggaagagatc gaggggtgag agagcctgct tcgtgttctt cgtgcatctc tttgtggtct
     2341 tctggtgaaa ggggagccat atgcggcaag catggcgatg gtacggtccg cacgacaatg
     2401 tctcgctcga cgacgtgcgc caggcgggcg cgagcgagat cgtcagcgcg ctgcacgcca
     2461 ttccgcccgg cggcgaatgg acgagagagg ccgtcgccga gcgcaaaagg atcgtcgaag
     2521 agacgccatt cggccgcacg ccgctgaaat ggacgattgt cgagagcatt ccggttcctg
     2581 acgacgtcaa gcgcttcggc aaggcggcga aagcggcgat cgatgtctgg atcgccagcc
     2641 tggaggcggt cgccgccaac gggatcacga cggtctgtta caatttcatg ccggtcctcg
     2701 attggacgcg gaccgatctc gattggctct tgcccaacgg cgccaaggcc ttgcgcttcg
     2761 acgcggcgcg cctcgccgcc tacgacattc acatcttgaa gcgccccggc gccgcgcagg
     2821 actatgcgcc tgacgtgcgc gagcgcgcgc gccgaacctt cgaaaagctc tcgcaaggcg
     2881 aggcggagcg gctgatcgcc aatatcggcg ccggcctgcc gggaacgacg agcgagccgg
     2941 tcgacgccgc cggcatgcgc gagaagctcg cccaatacgg caagatcgac gcggcgcaat
     3001 tgcgccgcca tctggtcgag ttcctcgaac gcgtcgcgcc ggccgccgag gcgctcggcg
     3061 tcaagctgac gctgcatccc gacgatccgc cgcggcccct tcttggcctg ccgcgcgtcg
     3121 cctcgaacgc cgaggactat caggccttgt tcgacgccgt gccgacgccg gccaacggca
     3181 tgtgcttctg caccggctcg ctcggcgcct cgccggccaa cgacctgccg gccatcgccc
     3241 gccgcttcgc gccgcgcatc tatttcgcgc atctgcgcgc ggtgaagcgc gagccggacg
     3301 gctccttcta cgaggccgac catctcgacg gcgattgcga catggtcgcg gttttgcgcg
     3361 tccttctcag cgaggatcgc cggcgcggat cggaagagaa gattcccttc cgcccggatc
     3421 acggccacat catgctcgac gatctgcgca agaagaccaa tcccggctat tcggcgatcg
     3481 gtcggctgaa agggctggcg gagctgcgcg gcgtcattcg agcgctggag agcctgtagc
     3541 cgcgctcgtt gagcgcggct gacgcggtcg ccgaccgcat ctacaacgcc tagagcctat
     3601 cccttcaacg ccgccagctg cgacaactgc ccgagcagcg cgcgcctgtc gctctcgtcg
     3661 ccgatcgcgg cggcgatgcg cgcttcgtgc gcgatgcgca ccttatgcag tccggcgagc
     3721 tcggcttggc cggttttggt caggaacaag gcgttggcgc gccggtcgcc cgcgacctct
     3781 ttgcgtacga cgagcttgcg ccgttcgaac tcgtcgatca gcttgacgaa attggaccgt
     3841 ttgatgccga gcgactgcgc gatcgccgcc tgcgaggagc cgggattgct ctcgatcgcg
     3901 gtcaggacgc catattgcgt cggccggatg tcgtaaggcg cgaaggagcg gatgaaatcg
     3961 tcgaacaccg cgatctgggc gcggcgcaat tgatagccga tcaggtcggt cagcttggag
     4021 agatcgaccg cctggccatt gacggcgcgc ggcgccgcct cgtcctgggt gagcgtctcg
     4081 tcggtcaatc taagcgctcc ggccgcctca acgccatccg ccaaggccga atatagttat
     4141 gctgtatcgc aattcgaatg cgacgcaaag ccgccctggc ggatcaggcc gccaggtctt
     4201 atttcgggtc ggatttcgcg cgataattgc gccttagcct ttccggagct ccccccgtga
     4261 gttcgcctgc gccgtcgtcc ccgcccatgc tccccggcgg ccgtcgggtt ttcatcgtga
     4321 tcgtctcggc cgccttggtc ctggccgcga gcctcgccta tgttttgacg agcgtgcgcg
     4381 ccggcgcgcc ggccgcctcg ctgatcggcg gcccgttcaa gctcgtcgcc ggcgacggcc
     4441 gcacaatcac cgacgccgac atgaagggcg agccgttcct ggtctttttc ggctacacgc
     4501 attgcccgga cgtctgcccg gcgacgctcg ctgaaatctc ggacgtgctg gcgaaaatgc
     4561 ccggcaagcc ggtcaaggcc ttgttcatca ccatcgatcc cgagcgcgac agcgcggcgg
     4621 tgatgaagga ttacgtctcc aattttgatc cgcgcatcat cggcctgacc ggcgaccgcg
     4681 ccgcgatcga tcaggtcgaa aaagcctatc gcgtctacgc ccgcaaggcc ccccaggccg
     4741 gcggcgacta caccatggat catagcgcga tcgtctatct gatggattcg caggggcgct
     4801 tcgtcgaggc cttcaatctc gaacgcaaac cggaggactc ggcgaaggag ctggagaagt
     4861 atctttagac ccgctcgctg agcgcggccg acggtcatcg accgcgtctg cagcgcgccg
     4921 cctcaccgcc gcccgaacag gcgctcgata tcggcgagct tcagctccac ataggtcggc
     4981 cggccgtgat tgcattggcc ggcgccgggc gtgcgctcca tctcgcgcag cagcgcgttc
     5041 atctcttccg gcttcagtcg gcgtccggcg cgcaccgaat ggtgacaggc gatggtcgcc
     5101 aggcgatggt cgagccggcg ctcgagcgag agcgcgccgt cttcggcggc gagttcgtcg
     5161 gcaaggtcgc tgatcagcgc gccgatatcg gcgccgtcga tcagcgccgg aacctcgcgc
     5221 accaggaccg cgccggggcc gaagccttcg acgatcagcc cgagctcgcg tagagactcg
     5281 gcgcgctcgg cgacgagcgc ggcggcgtcg ggatcgagat cggcgacaaa gggcgtgagc
     5341 aggatttgcc gcgcaacgcc gctctcggcg cgctggcgct tcaagcgctc atagacgatg
     5401 cgctcatgcg cggcgtgctg gtcgacgatc acgacgccgt ccgccgtctg ggcgatgaca
     5461 taggaatcgt gcacctgcgc gcgcgccgcg ccgagcggcg cggcgagatc ggcggcggcc
     5521 tcgaccggct cggctttgat cgccgcggcc ggcgcgaagc cgtcgaaggc ctgctgcgcc
     5581 ggctcggcga agcccagcgg cgccgcgggc gaggcgcgcc aatcccagcc gccattggcg
     5641 ggacgaggcg agcccgaaaa gccgttcggc gcgcgcaagg cggcgatggc gccgtcgccg
     5701 ccggtcgtcg cggcgcgatg cagcgccgct tgcagcgcgg ctttcagcgc gctgacgatc
     5761 aggccgcgga cgaggccggg atcgcggaaa cgcacttccg ccttggccgg atggacattg
     5821 acgtcgacct cgcgcggatc gcattcgatg aataaggcca cggccggatg gcgctcgcgg
     5881 ggcaggtagt cggcgtaagc ggcgcgcagc gcgccggcga gcagcttgtc gcgcaccgtc
     5941 cggccgttga cgaagaaata ttgctgaagc gcgttggcgc ggtggtaggt cggcaggccg
     6001 gcgaagccgg agagcttgac gccctcgcgc tcggcggcga tggcgagcgc attgtcggaa
     6061 aattcagcgc cgagcgcctg gcgcgtccgc gccgcccggc cttcctcgtc cggcgcgcag
     6121 gccggccaat cgaagccttg gctcgcctcg ctggcgaagg agaagcgcac ggcgggattg
     6181 gcgagcgcga ggcggcgcac cacgtcgccg gcggcctgcg attcggcgcg ctcgctcttc
     6241 aagaatttca gccgcgccgg cgtcgcggcg aaaaggtcgc gcgcctcgat ccgcgtccct
     6301 tgcggttgcg cgcagggctg gacctcgccc ttgaccccgt cctcgatgcg gatcaagaac
     6361 gcttccgtcg ccccggccgc tcggctgcgg agttcgaggc gcgacaccga ggcgatcgac
     6421 ggcagcgctt cgccgcggaa gccgaaggtc gcgatcgcgg aaaggtcgcc gtcgggaatt
     6481 ttcgaggtgg cgtggcgctc gacgcagagg gcgagatcgg ccgcgcccat gccgcttccg
     6541 ttgtcgatga cgcggatgag cgagcgcccg cccgcttcga tcgccacgtc gatccgagtc
     6601 gcgccggcgt cgagcgcgtt ctcgacgagc tccttcaccg ccgccgcggg ccgctcgatc
     6661 acctcgccgg cggcgatgcg gtcgatcagg agcggatcga ggcggcggat gggcatcggc
     6721 agacctgtct tcggggcgcc gcgcgccaga gacgattcgc caagtttaga gcaagcgcgc
     6781 cggaaccgcg agttcggcgg cggcctctgc aagcccgcga tcaagcgtca cgattgtcgc
     6841 gccatgattt gcggcaattg cgagatgcag cgcgtcgccg ggcgcaatcc aagctttgct
     6901 ggtcagcgta tgtcgccggc ggcgaggcgt tcaacgagct cgctgagacg ggccttcgcc
     6961 tcggcaagac taataccgcg ctctttgcct ctcatggcca tctacatggt catgtaacgc
     7021 aatcgacaag aacgttcacg gcgttgacgg attaggatgt ttcagctctg cgcttgcgct
     7081 cagcgcccct cacgcggtcc gcaaatcaga gcggccccgc catttgccat tccgcctgct
     7141 tcaacgaaag cgagatggct ctgctttgta gtaccccttt ttggccggcc ggcgccgatt
     7201 ctgtttcctt gattgcgcga gggtttcaca aaaccatcgg tccataaatt ttattttatg
     7261 ggagagacag atggtcaagt tggcgatcgc agccgccgct ggggcagtgt ttttggcgac
     7321 gacgcttgcg ccggcggccg ccggtcagcg gggccaggcg agcctgaaca tcccgatgtg
     7381 caagcaggcg gtcgccgcca agcacttggc ggacaaggcg gcgcgccagg ccgaat
//
LOCUS       DATGJW010000272         7228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_673121, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000272 DATGJW010000000
VERSION     DATGJW010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7228
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673121"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VKU03_08405"
     CDS             complement(<1..568)
                     /locus_tag="VKU03_08405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLJ71324.1"
                     /translation="MERVLGNKIPEFNRKEEASEPSLAGLNSFGLGGSRTHLETLVII
                     LVAVVVIGGMELLLRIFNVPQYILPKPSQIVAALFTEWPFLWPHLVITLYELFVGFAI
                     GGAIGFVLAAVITQFPFVEKVVTPYILLLVTTPMLALVPLLILRFGFGSEPRIIAVAL
                     ASGPMVMINSATGFRRVDLAKIALARSFG"
     gene            complement(586..1386)
                     /locus_tag="VKU03_08410"
     CDS             complement(586..1386)
                     /locus_tag="VKU03_08410"
                     /EC_number="7.6.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71325.1"
                     /translation="MAAGASERPKMTVEAATKFYATRSGSVHALDKVSLDLREGEFLC
                     ILGPSGCGKSTLLWSMAGLHDLTSGQIRLGSEVIAGPHPEIAMIFQDANLLPWRNLAK
                     NVALPFELKRRRPDMARIGRLLERVNLKGFENKFPRELSGGMQQRASIVRSLAVDPSV
                     LLMDEPFGALDAFTRDEMNLLIQEIWMETGKTIAFVTHSISEAIFLADRVVVMSARPG
                     RVAAIYDIDIPRPRPVEIQTQPDFIARVLEIKSRIDRGRVASEGLAIA"
     gene            1686..2639
                     /locus_tag="VKU03_08415"
     CDS             1686..2639
                     /locus_tag="VKU03_08415"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HLJ71326.1"
                     /translation="MQARRHSEDGPRIDPVAPIDLVSRIQRVEGDLSPAERRVAEAVA
                     VDYQMTTRMTIAELARRAGVSQPSVTRFCRSVGCASFGEFKVRLATTLTVAAVYLRTD
                     RVFDDDVGQLAQLVMLRAANAIRNCLDQLDTAAVKRAVAAIAACARLDIYGQGGGSAS
                     LAEDAKLRLFRLGIPVCAYCDGHQQRMSAATLRPKDAVFAISNSGRSKPVVDAVKIAR
                     SYGATTIALTRPQTPLADAAEIVIPVVVPEDANALMPTASRYAQMAVIDTIATGVASE
                     MGARSRESLRRVRYTLASIGVAIPSPSSDPTPLMRGAKAQE"
     gene            2673..4472
                     /locus_tag="VKU03_08420"
     CDS             2673..4472
                     /locus_tag="VKU03_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLJ71327.1"
                     /translation="MMKTQEAATAVETWLSAFDEALRSRDGEAASALFVEDCFWRDLV
                     AFTWNIKTLEGREAIAAMLRANLDATAPSRWRLTEAPERRDGVCEAWLSFETQVGRGR
                     GVLRLRDGKAWTLLTALEELKGFEERSGPRRPNGVEHGVYRREKTWLEAKQAEEAALG
                     YEKQPYCVIVGGGQGGIALGARLKQLDVPTIILEKNALPGDSWRNRYRSLVLHDPVWY
                     DHLPYIPFPDHWPVFTPKDKLGDWLEMYVKVMELNYWAASECLRAAYDEEREEWIVEV
                     RRDGRPATLRPKHLIFATGAYGPPREIPLPGRERFRGECYHSSRYQTGEAYAGKHCVV
                     IGGNSSAHDIAANLLEFGAKVTMLQRSPTTVVRSETLMEYGFGPLYSESALKAGISAE
                     KADLIFAATPFRLMPQGQIELYREIRKRDAAFYERLAKSGLRLDFGEDESGLMMKAFR
                     TGSGYYIDVGASELIANGSIAVKSGVEIRQVKENAVELTDGSELPADLIVMATGFQSM
                     NRALAPIISEEVADRVGKCWGLGSGVANDPGPWEGEPRNMWKPTQQPALWLHGGNLHL
                     SRHYSLLLALQLKARMEGLPTPVYGLQPVFHKS"
     gene            4497..5984
                     /locus_tag="VKU03_08425"
     CDS             4497..5984
                     /locus_tag="VKU03_08425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014498345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLJ71328.1"
                     /translation="MKDDDLAYMSAAETAELVRRRRLSPVETVEAAIARIERRNPSLN
                     AFVFKGFDDARREAKEAERQVMAGADLGPLHGVPSAIKDLFDFKPGWPYTFGGIRAMK
                     NCIAQWRCIFAERVEKAGAIFLGKTNSPTMGLRGTCDNYLFGPSRNPFDTRKNTGGSS
                     GGSAAAVADGLVPLAEGTDAGGSIRIPSAWCNVVGFKASFGRVPVAMRPNAFGGETPF
                     VFEGPISRTVEDAALALNVLAGADSRDPFSFETREDFLVSMRRSIKGWRIAYTPDFGV
                     FPIDPAIRKTVGEAVRAFTDAGATVEEATFGLKRSQRELSDVWTRLMTPLNISALEGM
                     KASGLDLWGDHRDDFPPAYIERIEKGRSLSAMDIVHDQAIRSEVYDAIEAVLAKYDLI
                     VSPTLAAMPVDNAADGDTKGPTHVDGVEVDPLIGWCLTYLTNFSGHPSASIPAGLSGG
                     LPVGMHLIGRRHADGDVLAAAAAFERVKPWRDAYRICANRPIPAA"
     gene            complement(6010..7032)
                     /locus_tag="VKU03_08430"
     CDS             complement(6010..7032)
                     /locus_tag="VKU03_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014131756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ71329.1"
                     /translation="MSITGLGPHGERAAPADKVSLSEAEAAAARAGRFAIAVVLHTTT
                     SDWSRQELAGIVATLGAYSAAVVEVVDCAYDKQKQNRELLRLARSRVNAVISIPIGAE
                     GVVDGHRAVSASGKTLVLLDNVPAGLMPGADYAAVVSADNFNLGEIAAELLSPHLPEE
                     GVAGILTYGADFYATNEREIGFRKWIGRRRPDVTLVRGRFLSVEEAGAAFDRLLAEND
                     DLDGLFVAWDVPALNALAAIRARARKLAVATVDLGNAAAAELIKGDLLKGIAAQRPYD
                     QGAAAALVTLLNLVGRRPPPWIAAAGMPATSEDVLGAYQVVWHAPAPNDLLEARQRML
                     ASQAAR"
BASE COUNT         1230 a         2398 c         2392 g         1208 t
ORIGIN      
        1 cgccgaaaga gcgcgccagg gcgatcttgg cgagatcgac ccggcggaag ccggtcgccg
       61 aattgatcat caccatcggg ccgctcgcca gcgccacggc gatgatgcgc ggctcgctgc
      121 cgaagccgaa gcgcaggatc agaagcggca ccagcgccag catcggcgtc gtcaccagca
      181 gcaggatata cggcgtgacg accttttcga cgaacggaaa ttgcgtgatc accgccgcca
      241 ggacgaagcc gatcgcgccg ccgatcgcga agccgacgaa caattcgtac agcgtgatca
      301 cgagatgcgg ccacaagaat ggccattcgg tgaacagagc ggcgacgatc tggctcggct
      361 tgggcaggat gtattgcggc acgttgaaga tgcgcagcag cagctccatc ccgccgatca
      421 ccacgacggc gacgagaatg atgaccaggg tctccaaatg agtccggctg ccgccgagcc
      481 cgaaagaatt gagacccgcc aggcttggct cgctcgcctc ctccttgcga ttgaattcag
      541 ggattttgtt gccgagaacc cgctccaccc catcctccga attgctcagg cgatcgccaa
      601 tccctcggac gcgacgcggc cgcgatcgat tctgctcttg atttcgagga cccgcgcgat
      661 gaaatcgggc tgggtctgaa tttcgacggg gcgcggccgc ggaatgtcga tgtcgtagat
      721 cgcagcgacc cggcccggcc gcgccgacat cacgacgacg cggtccgcca gaaagatcgc
      781 ctcggaaatc gagtgcgtga cgaatgcgat cgtcttgccg gtctccatcc agatttcctg
      841 gatgagcagg ttcatctcgt cgcgggtgaa ggcgtcgagc gcgccgaacg gctcgtccat
      901 caaaagcacc gacggatcga cggcgaggct gcgcacgatc gaggcgcgct gctgcatgcc
      961 gcccgagagt tcgcgcggaa acttgttctc gaaacccttg agattgaccc tttcgagcag
     1021 ccggccgatg cgcgccatgt ccggacgacg ccgcttgagc tcgaagggca gggcgacgtt
     1081 cttggccaga ttgcgccaag gcagcagatt ggcgtcctgg aagatcatcg cgatttccgg
     1141 atgcggtccg gcgatgacct cgctgcccag cctgatctgg ccgctggtca gatcgtgcaa
     1201 gcccgccatc gaccaaagca aggtcgactt gccgcagcct gaaggaccga gaatgcacag
     1261 gaactcgccc tcgcgcaggt cgagcgacac tttgtcgagc gcgtgtaccg agcccgaacg
     1321 cgttgcgtag aatttggtcg cggcctcgac ggtcatcttt ggccgttcgg atgcgccagc
     1381 ggccatctct tcctcctcgc ggcgcccttt ggcccgtctt ggcgacggtt gtccaaattc
     1441 ggcggcggcg ccgatgtcga tcgctgaaat tatgttacac aagctttgtg cgcgcaagcg
     1501 gaaagttgcc cgccggctcg gcggcggcat gacgcgttct cccgttcccc cgcggcttgc
     1561 gcgggaaatc ccgtctctct tcccctggag cgtgtcttgt catcgccgat cagacgtgta
     1621 atttaattac aggttcgccg cggggctcga ctcaacgaag cctcggcgcg gccggaggaa
     1681 atcgcgtgca ggcgagacgg cactcggagg acggtccgcg catcgatcca gtcgcgccga
     1741 tcgatctcgt ctcgcggatt cagcgcgtcg agggcgactt gtcgccggcc gagcggcgag
     1801 tggccgaggc ggtcgcggtc gactatcaga tgacgacgcg gatgacgatc gccgagctgg
     1861 cgcgccgggc cggcgtcagc cagccgagcg tcacgcgctt ctgccgctcg gtcggctgcg
     1921 cctctttcgg cgaattcaag gtacgtctgg cgacgacgtt gacggtcgcc gccgtctacc
     1981 tgcggaccga ccgcgtcttc gacgacgatg tcggccagct cgcgcaactg gtgatgctgc
     2041 gcgccgccaa cgccatccgc aattgcctcg atcagctcga caccgccgcg gtcaagcgcg
     2101 cggtcgctgc gatcgccgct tgcgcgcgtc tcgatattta cggccagggc ggcggctcgg
     2161 cgagcctggc ggaagacgcc aaattgcggc tttttcgatt gggaattccg gtttgcgcct
     2221 attgcgacgg ccatcagcag cgcatgtcgg ccgcgacgct gcggccgaag gacgcggttt
     2281 tcgccatatc gaatagcggc cgatcgaagc cggtggtcga tgcggtcaag atcgcccgct
     2341 cctatggcgc cacgacgatc gcgctgaccc ggccgcaaac gccgcttgcc gacgccgccg
     2401 agatcgtcat cccggtcgtc gtcccggaag atgcgaacgc gctgatgccg accgcttcgc
     2461 gatacgctca gatggcggtg atcgacacga tcgcgaccgg cgtcgcctcg gaaatgggcg
     2521 cgcgctcgcg cgagtcgctg cgccgcgtgc gctacacgct cgccagcatc ggcgtcgcca
     2581 ttccaagccc gtcctccgac ccgacgccgc tgatgcgcgg cgcgaaagcg caggaatagc
     2641 aggaaggcgt gtgatcgaag aggaagctga agatgatgaa aacgcaagag gccgcgaccg
     2701 ctgtcgagac ctggctttcc gctttcgacg aggcgctgcg ctcgcgcgac ggcgaggcgg
     2761 cgagcgcact gttcgtcgag gattgtttct ggcgcgatct cgtcgccttc acctggaaca
     2821 tcaagacgct ggagggacgc gaggcgatcg cggcgatgct gcgcgccaat ctcgacgcga
     2881 ccgcgccgag ccgttggcgg ctgaccgaag cgcccgagcg ccgcgacggc gtctgcgaag
     2941 cctggctcag cttcgagacc caggtcggcc gcggccgcgg cgttctgcgc ctgcgcgacg
     3001 gcaaagcttg gaccctgctg accgcgttgg aggagctcaa gggcttcgag gagcgcagcg
     3061 gcccgcggcg tccgaacggc gtggagcacg gcgtctatcg ccgggagaag acctggctcg
     3121 aggccaaaca agccgaggag gcggcgctcg gctacgaaaa acagccctat tgcgtcatcg
     3181 tcggcggcgg ccagggcggc atcgcgctcg gcgcgcggct caagcagctc gacgtgccga
     3241 cgatcatcct cgagaagaac gcgctgcccg gcgattcctg gcgcaaccgc tatcgctccc
     3301 tggttctgca cgatccggtc tggtacgatc acctgcccta catccccttt cccgatcact
     3361 ggccggtttt cacgccgaag gacaagctcg gcgattggct ggaaatgtac gtcaaagtga
     3421 tggagctgaa ctattgggcc gccagcgaat gcctgcgcgc cgcctacgac gaagaacgcg
     3481 aggaatggat cgtcgaggtt cgccgcgacg gccgtccggc gacgctgcgg ccgaaacacc
     3541 tgattttcgc gaccggcgcc tatggcccgc cgcgggagat tccgttgccc ggccgcgagc
     3601 gcttccgcgg cgagtgctat cattcgagtc gctaccagac cggcgaggct tacgccggca
     3661 agcattgcgt cgtgatcggc ggcaacagct ccgcccacga catcgccgcc aatcttttgg
     3721 agttcggcgc gaaggtgacg atgctgcagc gctcgccgac cacagtcgtg cgctcggaga
     3781 cgctgatgga gtacggcttc gggccgctct attcggaaag cgcgctgaag gcggggatca
     3841 gcgccgagaa ggccgacctc atcttcgccg cgacgccgtt ccggctgatg ccgcagggac
     3901 aaatcgagct ctatcgcgaa atccgcaaac gcgacgcggc tttttacgag cgcctggcga
     3961 agtcggggct ccgcctcgat ttcggcgagg acgaatcggg cctgatgatg aaggcgtttc
     4021 gcaccgggtc gggctattac atcgatgtcg gcgcgtccga attgatcgcc aacggctcga
     4081 tcgcggtgaa aagcggcgtc gaaatccgtc aggtgaagga gaatgcggtc gaattgacgg
     4141 acggctccga attgccggcc gatctcatcg tcatggcgac cggcttccag tcgatgaacc
     4201 gcgcgctcgc gccgatcatt tcggaggaag tcgcggacag agtcggtaag tgctggggcc
     4261 tcggctccgg cgtcgccaac gatcctggtc cgtgggaagg cgagccgcgc aacatgtgga
     4321 agccgacgca gcagccggct ctgtggctcc atggcggcaa cctccatctg tcgcgccatt
     4381 attcgctgct tctggcgctg caactgaagg cgcgcatgga ggggttgccg acgcccgttt
     4441 atgggctgca gcccgtcttt cataaaagct gaagatcaaa gcttgggagg tatgtcatga
     4501 aggatgacga tctcgcctat atgagcgccg ccgaaaccgc cgaactggtg cgccggcgcc
     4561 gtctttcgcc ggtcgaaaca gtggaggccg cgatcgcgcg catcgagcgt cgcaatccga
     4621 gcctcaacgc tttcgtcttc aagggattcg acgatgcgcg gcgcgaagcg aaggaagccg
     4681 agcgccaggt catggcgggc gcggatctcg ggccgctgca tggcgtgcct tcggcgatca
     4741 aggacctgtt cgatttcaag cccggctggc cgtacacgtt cggcgggata cgggcgatga
     4801 agaactgcat cgcgcaatgg cgctgcatct tcgccgagcg cgtcgagaaa gccggcgcca
     4861 ttttcctcgg caagaccaac agcccgacca tgggcctgcg cggcacctgc gacaactatc
     4921 ttttcggccc gtcccgcaac ccgttcgata cgcgcaagaa taccggcggc tcgtccggcg
     4981 gcagcgccgc ggcggtcgcc gacgggctcg tcccgctcgc cgaggggacc gacgccggcg
     5041 gctcgatccg catcccttcg gcttggtgca acgtggtcgg cttcaaggct tcgttcggcc
     5101 gcgttccggt cgcgatgcgg ccgaacgcct ttggcggcga gacgcctttc gtcttcgaag
     5161 ggccgatcag ccgcacggtc gaggacgcgg ccctcgcgct caacgtcctt gccggcgccg
     5221 attcgcgcga tcccttttcc ttcgagacgc gcgaggactt tctcgtctcg atgcgccgct
     5281 cgatcaaagg ctggcgcatc gcctacacgc cggatttcgg cgttttcccg atcgatccgg
     5341 cgatccggaa aacggtcggc gaggcggtcc gcgctttcac ggatgccggc gcgaccgtcg
     5401 aagaggcgac gtttggcctc aagcgcagcc agcgcgaatt gagcgacgtc tggacgcggc
     5461 tgatgacgcc gctcaatatc agcgcgctgg aggggatgaa ggcctcgggc ctcgatctat
     5521 ggggcgacca ccgcgacgat tttccgcccg cctatatcga gcggatcgaa aagggccgct
     5581 cgctttccgc catggacatc gttcacgatc aggccatccg cagcgaggtc tatgacgcga
     5641 tcgaggcggt gctggccaaa tacgatttga tcgtctcgcc gaccctcgcc gcgatgccgg
     5701 tcgacaacgc cgcggacggc gacacaaagg ggccgacgca tgtcgacggc gtcgaggtcg
     5761 atccgctgat cggctggtgc ctgacctatc tgaccaattt cagcggccat ccgtcggcct
     5821 ccattcccgc cgggctcagc ggcggtttgc cggtcggcat gcatctgatc gggcggcgcc
     5881 acgccgacgg ggacgtgctg gccgccgccg cggcgttcga gcgcgtcaag ccgtggcggg
     5941 acgcctatcg gatctgcgcc aaccggccga tacccgccgc ctgaggcgcg cgcccgcgcc
     6001 ggccggcgtt caccgcgccg cctgcgacgc gagcatccgc tggcgcgcct caagcaagtc
     6061 gttgggcgcc ggcgcgtgcc agacgacctg gtacgcgccg agcacgtcct cgctcgtcgc
     6121 cggcatgccc gccgccgcga tccagggggg cgggcgtcgc ccgaccaggt tgagcaaggt
     6181 cacaagcgcc gcggccgccc cttgatcata gggacgctgg gcggcgatgc ccttgagaag
     6241 gtcgcccttg atcagttcag cggccgcggc gttgccgaga tcgaccgtgg cgaccgcaag
     6301 cttgcgggcg cgggcgcgga tcgccgcaag agcgttcagc gccggcacgt cccaggcgac
     6361 gaaaagcccg tcgagatcgt cgttctccgc gagaagccgg tcgaaagcgg cgcccgcttc
     6421 ctcgaccgag aggaagcggc cgcgcaccag tgtcacgtcc ggccgccgcc ggccgatcca
     6481 tttgcgaaag ccgatctccc gctcgttggt cgcgtagaaa tcggcgccgt aggtgagaat
     6541 gccggcgacg ccttcttccg gcagatgcgg cgacaggagt tcggcggcga tctcgccgag
     6601 attgaaattg tcggcggaga ccaccgccgc gtagtcggcg cccggcatca atcccgccgg
     6661 gacgttgtcg agcagaacca gcgtctttcc ggacgccgac acggcgcggt gcccgtcgac
     6721 gacgccctcg gcgccgatcg ggatgctgat caccgcattc acccggctcc gggcgagccg
     6781 cagcaattcg cgattctgct tctgcttgtc gtacgcgcaa tcgacgacct cgacgaccgc
     6841 ggcggaatag gcgccgagcg tcgcgacgat tcccgccagc tcctgccgcg accagtcgct
     6901 cgtcgtcgtg tgcaggacca cggcgatggc gaaccggccg gcccgcgcgg ccgcggcttc
     6961 ggcctcggac agggagactt tgtcggccgg cgcggctctt tcgccatgcg gtccgagtcc
     7021 ggtgatggac attcgctttt ctcgcctccg tccactaggc cgctaaattt cccgcgatgc
     7081 aagccgctcc gccgcgctca cgaatccgct gcgatttgcg cgcgcggcgg ccgcggcgca
     7141 ggcgcagcgg cgcgccgctt gcctcgctcg gtctccaaga attccagaaa cgccgggcgc
     7201 cgccgcggcg agccatgcgc gatccaat
//
LOCUS       DATGJW010000273         2125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_673355, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000273 DATGJW010000000
VERSION     DATGJW010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2125
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_673355"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1213)
                     /locus_tag="VKU03_08435"
     CDS             complement(<1..1213)
                     /locus_tag="VKU03_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HLJ71330.1"
                     /translation="MPSPEPLALIDLARRGDKAGRDVATAEGALRFSDFAAATALGGA
                     REKLAGRSVLLLAGDSLKTAAALIELDGVARRLILCPPDLRADRIGDVAAIAEADAVV
                     HGGEEPPGLDVAIAAPCRLPLHAAPTTALPRLATEWLLLTSGTTGAPKLVSHDLATLT
                     GAFAGGPAGEIQNWATFYDIRRYGGLQILLRAIAGLGSLTLTSAGEPIDEFIDRAIAR
                     SVTHISGTPSHWRRVLMSRAASRFKPAYVRLSGEIADAAILGALKELFPEARIAHAYA
                     STEAGVGFEVNDGEPGFPASYLGERDNGVAMKIEDGSLRIRSPRAARRYVGRNMPDLR
                     DEDGFIDTGDMIERRGERCHFVGRRGGVINVGGAKVHPEEVEAVINRQAGVRMSLVKA
                     RRNPIAGAIVVA"
     gene            complement(1200..1445)
                     /locus_tag="VKU03_08440"
     CDS             complement(1200..1445)
                     /locus_tag="VKU03_08440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLJ71331.1"
                     /translation="MSLRLIIVSQIERVAREQRKTIAPLADDLPLLESGLDSLCFAIL
                     VARLEDEVGVDPFSASPEAGFPTTFGEFVKFYENAVA"
     gene            1532..>2125
                     /locus_tag="VKU03_08445"
     CDS             1532..>2125
                     /locus_tag="VKU03_08445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA acyltransferase"
                     /protein_id="HLJ71332.1"
                     /translation="MTAPVRNRVKCRQIEEADLARVAERLAHAFPARSPAYWTAALER
                     MRRRPILENYPRYGVLLESEGRVVGVLLQIFFRRGEGDAAAVYCNLSSWCMDPQFRSY
                     AFTLNSAATTRKDVTYLNVSPAPHTRPGIEALGFRRYCDGQFVCLPWLALSKRGAARV
                     LSFAPDRPEAALLSACDRDILDKHAALGCRSLIVMAEG"
BASE COUNT          350 a          707 c          730 g          338 t
ORIGIN      
        1 ccgcgacgac gatcgcgccg gcgatcggat tgcgccgcgc cttgaccagc gacatgcgca
       61 cgccggcctg gcggttgatc accgcttcga cttcctccgg atgcaccttg gcgccgccga
      121 cattgatcac gccgccgcgc cgcccgacga aatggcagcg ctcgccgcgc cgctcgatca
      181 tgtcgccggt gtcgatgaac ccgtcctcgt cgcgcaaatc cggcatgttg cggccgacat
      241 agcggcgcgc cgcgcgcggc gagcggatgc gcagggagcc gtcctcgatc ttcatcgcga
      301 cgccgttgtc gcgctcgccg agataggagg ccggaaaacc cggctcgccg tcattgactt
      361 cgaagccgac ccccgcctcg gtcgaggcat aggcatgggc gatgcgcgcc tccgggaaga
      421 gctccttcaa cgcgccgaga atggcggcgt cggcgatctc cccggacagg cgcacatagg
      481 cgggcttgaa gcgagacgcg gctcggctca tcagaacccg ccgccagtgc gacggcgtgc
      541 ccgaaatatg ggtgaccgac cgggcgatcg cccggtcgat gaattcgtcg atcggctcgc
      601 cggccgaggt cagcgtcagc gagcccaagc cggcgatcgc ccgcagcagg atctgcaggc
      661 cgccatagcg gcggatgtcg tagaacgtcg cccagttctg gatttccccc gcggggccgc
      721 cggcgaaagc gccggtcagc gtggcgaggt cgtgcgacac cagttttgga gcgcctgtcg
      781 tgcccgacgt gaggagaagc cattcggtgg cgaggcgggg cagggccgtc gtcggcgcgg
      841 cgtgcagcgg caaccggcag ggcgcggcga tggcgacatc gaggccgggc ggctcttcgc
      901 cgccatggac gaccgcgtcc gcctcggcga tggcggcgac gtcgccgatc cggtcggcgc
      961 gaagatcggg cgggcagagg atcagccggc gggcgacgcc gtcgagctcg atcaacgccg
     1021 cggccgtctt caaggaatcg ccggcaagga gcagaaccga gcggccggca agcttctcgc
     1081 gcgcgccgcc gagcgccgtc gccgccgcga aatccgagaa gcgcagcgcc ccttccgccg
     1141 tggcgacgtc gcgtcccgcc ttgtcgcctc gccgcgccag atcgatcagc gcgagcggct
     1201 caggcgacgg cattttcgta gaatttcacg aactcgccga acgtggtggg gaaaccggcc
     1261 tcgggcgaag cgctgaacgg atcgacgccg acctcgtctt cgagccgggc gacaagaatc
     1321 gcgaagcaca gcgaatcgag ccctgactcg agcaggggca aatcgtcggc gagaggggcg
     1381 atggttttgc gctgttcgcg cgcaaccctc tcgatttgcg agacaattat taacctgaga
     1441 gacatagaaa tagccttggg cgatcgctga agcgcaccct agcgcttcgc tggaatatcg
     1501 gttgaactgt ctgaattaac ggtaaatcgg aatgacggcg cctgttcgca atcgagtcaa
     1561 atgccgtcag atcgaggagg cggacctcgc tcgcgtcgcc gaacgcctgg cgcacgcgtt
     1621 tccggcgcgc tcgccggctt attggacggc ggcgctcgag cggatgcgcc gccgcccgat
     1681 tctcgagaac tacccgcgct acggcgtgct cctcgaaagc gaaggacgag tcgtcggggt
     1741 gctgctgcag attttcttcc ggcgcggcga gggcgacgcc gcggcggttt attgcaatct
     1801 ctcaagctgg tgcatggacc cgcaattccg cagctacgcc ttcacgctga attccgccgc
     1861 gacgacgcgc aaggatgtga cgtacctcaa tgtctcgccc gcgccgcata cccggccggg
     1921 catcgaggca ctgggctttc gccgctattg cgacggccaa ttcgtctgtc tgccctggct
     1981 ggctctttcg aagcgcggcg cggcgcgcgt gttgagcttt gcgcccgatc ggccggaagc
     2041 ggcgcttttg tcggcctgtg atcgcgacat tctcgacaag cacgcggcgc tgggctgccg
     2101 gtcgctgatc gtcatggcgg agggc
//
LOCUS       DATGJW010000274         4338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_258874, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000274 DATGJW010000000
VERSION     DATGJW010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4338
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_258874"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(163..2796)
                     /locus_tag="VKU03_08450"
     CDS             complement(163..2796)
                     /locus_tag="VKU03_08450"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phospho-acceptor
                     domain-containing protein"
                     /protein_id="HLJ71333.1"
                     /translation="MTGSHSPELGASQVAVLLAGTPFSAALAAGAPLLVAAVDPPRLV
                     YASDAAKALFGAATLDEIGEAAMKGASPGARRLRDLGRLLPIGAPPRLELLRFFAGRK
                     PLVVGLSCLLLTDSDGAAFLAATAPAPRASGGAPDTPEPAAEIAAASPPAGASDRFLW
                     SVDEAMRFAEPAASLAEALGEAAPRSGETIPALKSRLDLDGDGRLEAALAARATFNNL
                     RLAWPKASGGGQIILILSGAPLFDRERRFLGFRGFGRLTGETVAAAAQTAPPDQPSPS
                     HTAAPRGPAEPVQPPPVAPARLPPQQSAEIVPLRPPPVSGLAAQNVVPIRPDLRAIPP
                     VEEPQPSQSEASIALTPSERSAFREIARALGAKVSDEDAPETEADERADGGAPPDSDA
                     ARLLDRLPIGVLVARGDEALYLNRTLLDLLAYDDIEQFRAHSSVSRLFETEDIENLGP
                     AARPVALAAADGQTIAVEGLIQAIEWDGAPATLFSFRRAAAAEIAPRLAALEAEAKAR
                     ESELAAILNVAADAALALDRDGKILSLNRSGEALFGVEARDVLGEDFAALFTPKERDA
                     ARAAFEALRASGLESLADEGREMLALGKDGREIPLFMTMARIGTAAEAKFCAVMRDMT
                     RWKEIERDLEDARRQAEKASALKSEFLAKISHEIRTPLNAIIGFAEVMMEERFGPIGN
                     ERYKDYVKDIHGSGGHVMSLVNDLLDLSKIEAGKADLKLAPIDANLIIQECLTLMQPQ
                     AAHERVIMRASLAEKLPRVVADERSLRQIMLNLMSNAVKFNEPGGQVIVSTALNDSGQ
                     AVIRVRDTGVGMSEGEIATALEPYGRIATDRPAGGAGLGLPLTKALTEANRAQFSIRS
                     GKEQGTLVELAFPTTPAAAAQ"
     gene            3032..3391
                     /locus_tag="VKU03_08455"
     CDS             3032..3391
                     /locus_tag="VKU03_08455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin"
                     /protein_id="HLJ71334.1"
                     /translation="MTNYEIPNELRDFAERSVEQARKAFEGFVTVAQKAAGTIDGAAQ
                     QAQTDVKSMNTQIFGYAEQNVNAAFDFAHRLVQAKDPQEAFNLQNEYLKAQINALQAQ
                     AKEIGALIQKNAPGFKK"
     gene            3425..3880
                     /locus_tag="VKU03_08460"
     CDS             3425..3880
                     /locus_tag="VKU03_08460"
                     /inference="COORDINATES: protein motif:HMM:NF020918.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HLJ71335.1"
                     /translation="MVGSNSLAPLASQPNAPAAPGAALQEALREAAEKGLAESRAAYA
                     RARDAADQAASAFEASLAHAAQGVIDLNSKALDALRANFDAGVDFAKAAINSRTVGEL
                     AALHGDYASKQAEAFATQAREFGDLAQKIAAETMAPIKRQIGGMFGLSA"
     gene            3956..4032
                     /locus_tag="VKU03_08465"
     tRNA            3956..4032
                     /locus_tag="VKU03_08465"
                     /product="tRNA-His"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:3990..3992,aa:His,seq:gtg)
     gene            complement(4063..>4338)
                     /locus_tag="VKU03_08470"
     CDS             complement(4063..>4338)
                     /locus_tag="VKU03_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71336.1"
                     /translation="AWGYSFWGPAYGVAFRVDAAPPASETWRGSEAVNAAVFPGDAVA
                     FLDALPDRGAIEFHVADSFGRVHQARFRLRGVASVRRLIARACPPMR"
BASE COUNT          763 a         1411 c         1458 g          706 t
ORIGIN      
        1 catagaagag agaatatttc agcccgtgcg ttgatggcgg aacagggcgc ggcacgcgcc
       61 agaggaccat ctccacgatt gcgccgtcgt cgtaatcaaa gagtagatcg gccttgctca
      121 cgcctgtcta tggcatatag agccatatgg cgtcaatcac catcattgcg ccgcggcggc
      181 cggcgttgtc gggaaggcca gctccaccag cgttccctgc tccttgccgc tcctgatcga
      241 aaattgcgcg cgattggcct cggttagcgc cttggtcagc ggcaggccga ggccggcgcc
      301 gccggcgggg cgatcggtgg cgatccgccc gtaaggctcc aaggcggtgg cgatctcgcc
      361 ttcgctcatg ccgacaccgg tgtcgcgcac ccggatcacc gcctggccgg aatcgttcaa
      421 ggcggtggag acgatcacct ggccgccggg ctcgttgaac ttcaccgcgt tggacatcag
      481 gttcaacatg atctggcgca acgagcgctc gtcggcgacg acgcgcggca atttctcggc
      541 gagggacgcg cgcatgatca cccgctcatg cgccgcctgc ggctgcatca gcgtcaggca
      601 ttcctggatg atcagattgg cgtcgatcgg cgccaatttg agatcggcct tgcccgcttc
      661 gattttggac agatcgagca ggtcgttgac caggctcatc acatggccgc cggagccgtg
      721 gatgtccttg acgtaatcct tataccgttc gttgccgata ggaccgaaac gctcctccat
      781 catcacctcg gcgaagccga tgatggcgtt gagcggcgtg cggatttcat ggctgatctt
      841 ggcaaggaac tccgatttca gcgcgctcgc cttttccgcc tggcggcggg cgtcttcgag
      901 atcgcgctcg atctccttcc agcgggtcat gtcgcgcatg acggcgcaga atttggcttc
      961 cgccgcggtt ccgatccgcg ccatggtcat gaagagcggg atttcgcgtc cgtcctttcc
     1021 gagggccagc atttcgcgcc cctcgtccgc caagctttca agcccgctgg cgcgcagcgc
     1081 ctcgaaagcg gctctcgccg cgtcgcgttc tttcggcgtg aacaaagcgg cgaaatcctc
     1141 gccaagaacg tcgcgggctt cgacgccgaa aagcgcctcg ccggagcggt tgagcgagag
     1201 gatctttccg tcgcggtcga gcgccagcgc ggcgtcggcg gcgacattga gaatcgcggc
     1261 cagttcgctc tcgcgcgctt tcgcctccgc ttcgagcgcg gcgagccgcg gcgcgatttc
     1321 ggcggcggcc gcgcggcgga aggagaacag ggtcgccggc gcgccgtccc attcgatcgc
     1381 ctgaatgagg ccttcgaccg cgatggtctg gccgtcggcg gcggcgagcg ccaccggccg
     1441 cgccgcaggc cctaaattct cgatgtcctc cgtctcgaac aagcgcgaca cgctcgaatg
     1501 cgcgcggaac tgctcgatat cgtcataggc caaaagatcg agcaaggtac gattgagata
     1561 gagcgcttcg tcgccgcgcg cgaccagcac gccgattggc aggcgatcga ggagccgcgc
     1621 cgcatccgaa tccgggggcg cgccgccatc ggcgcgctca tcggcttcgg tctccggcgc
     1681 gtcctcgtcg ctgaccttgg cgccgagcgc gcgggcgatt tcgcgaaagg cgctgcgctc
     1741 gctcggcgtc aacgcgatgc tggcttcgct ctggctgggc tgcggctcct caaccggggg
     1801 aatggcgcgc agatcgggcc tgatcggcac gacattctgg gcggcgagcc cgctgaccgg
     1861 cggaggccgc aacggtacga tctcggcgct ttgttgcggc ggcaagcgcg ccggcgcgac
     1921 gggcggcggt tggacaggct cggccgggcc gcgcggcgca gccgtatggc ttggcgaggg
     1981 ttggtccggg ggcgccgttt gggccgctgc ggcgacggtc tcgccggtca agcgaccaaa
     2041 gccgcggaag ccgaggaagc gccgctcgcg gtcgaagagc ggcgcgccgg acaaaatcaa
     2101 aatgatttgc ccgccgccgc tggcctttgg ccaggcgagg cgcagattgt tgaacgtcgc
     2161 ccgcgcggcc aaagccgctt cgagccggcc gtcgccgtca aggtcgagac ggcttttcag
     2221 cgcgggaatc gtctcaccgc ttctcggcgc tgcctcgccc agcgcctcgg cgaggctcgc
     2281 cgccggctcg gcgaagcgca tcgcctcgtc gacgctccac aagaagcggt cgcttgcccc
     2341 ggccgggggc gatgcggcag caatttcggc ggccggctcg ggcgtgtccg gcgcgccgcc
     2401 gctcgcccgt ggcgccggcg ccgttgcggc gaggaaggcg gcgccgtcgc tgtcggtcaa
     2461 aagcaggcac gaaaggccga cgaccagcgg cttgcggccg gcgaagaagc gcagcaattc
     2521 gaggcgcggc ggcgcgccga tcgggagcag cctgccgaga tcgcgcaagc gccgcgcgcc
     2581 cgggctggcg cctttcatcg ccgcctcgcc gatctcgtcg agcgtcgctg cgccgaaaag
     2641 cgccttggcc gcgtcgctgg cgtagacgag gcgcggcgga tcgacggcgg cgaccagcaa
     2701 aggggcgccc gcggccaagg ccgccgagaa cggggtcccg gccagcaaga ccgcaacctg
     2761 cgacgcgccc agttctggcg aatgggagcc ggtcatcggg gtctggccac tccgttcggc
     2821 gcgatcgcgc caaagcgcgc aaccaattca aatcttacta aattattaac ggcgtcgagc
     2881 gccattccgc tagatgcgag aggcgcttgt cccaaacgaa aggatcgcgt ttggtgcgat
     2941 gcaataaaat gttgcaatgc agcaaaacct gtgctatggg ctctttcaca agcaaggaat
     3001 cgcccagcgc gcctgtccca aggagagaca catgacgaac tatgaaatcc ctaatgaatt
     3061 gcgtgatttt gccgagcgta gcgttgagca ggcgcgcaag gccttcgaag gctttgtcac
     3121 cgtcgcgcag aaagcggcgg gcacgatcga cggcgcggcg cagcaggcgc agacggacgt
     3181 caagagcatg aatacgcaga ttttcggcta cgccgagcag aatgtgaacg cggctttcga
     3241 tttcgcgcat cggctcgtcc aggccaagga tccgcaggag gcgttcaacc tgcagaacga
     3301 atatttgaag gcgcagatca acgccttgca ggcccaggcc aaggagatcg gcgcgctgat
     3361 tcagaaaaac gcgcccggtt tcaaaaagta gacgaacatt cctctttctt cgaaaggatc
     3421 ggtcatggtc ggctcgaaca gtcttgctcc tcttgcctcc cagcccaacg ccccggccgc
     3481 gccgggagcg gcgctccagg aggccttgcg cgaggcggcc gagaaggggc tggccgaaag
     3541 ccgggccgct tacgctcgcg ccagggatgc ggccgaccag gccgcttccg ccttcgaggc
     3601 gagcctcgcc catgcggcgc aaggcgtgat cgatctcaac agcaaggcct tggacgcgtt
     3661 gcgcgccaat ttcgacgccg gtgtcgattt cgccaaggcg gcgatcaaca gccgaaccgt
     3721 cggcgaactc gccgcgttgc acggcgatta tgcgagcaag caggccgaag ctttcgcgac
     3781 gcaagcccgc gaattcggcg acctggcgca gaagatcgcc gccgaaacca tggcgccgat
     3841 caagcgccag atcggcggaa tgttcgggct ttcggcgtaa tcatccttcc cgctgcgccg
     3901 cacggcggct cttgcaaccg ccgcgcataa attctaggtt ccggccgcat tcgctgccgc
     3961 catagctcag ctggttagag cgctagattg tggatctaga ggtcccccgt tcgatccggg
     4021 gtggcggtac caagcagacc cgctggcaat ccaaaaagcg tcctatcgca tcggcggaca
     4081 ggccctggcg atgagtcggc gcacgctggc gacgccgcgc aggcggaagc gcgcctggtg
     4141 aacgcgacca aaagaatcgg cgacgtgaaa ttcgatcgcg ccgcggtcgg gcagcgcatc
     4201 gagaaaggcg acggcgtcgc cgggaaaaac cgcggcgttg accgcttcgg agccgcgcca
     4261 ggtttcgctc gcaggaggcg cggcgtcgac gcggaaagcg acgccgtagg cgggtcccca
     4321 aaaggaatag ccccaggc
//
LOCUS       DATGJW010000275         5921 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1399132, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000275 DATGJW010000000
VERSION     DATGJW010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5921)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5921)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5921
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1399132"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(75..779)
                     /locus_tag="VKU03_08475"
     CDS             complement(75..779)
                     /locus_tag="VKU03_08475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1345 domain-containing protein"
                     /protein_id="HLJ71337.1"
                     /translation="MSIPSPAGGAPRRARFLDAVRHRPRLYICGAAAVLAYFALPHDL
                     RFATRALLAWNIGALLFIALVGWMMAQATEQSIRKRVALEDERQWVLLVVGTVAACAA
                     LAAIVSELGAVKDMTGWSKAAHIGLVTATILSAWTFIHLLFAIHYAHEYYGDEDDDLS
                     TPGVREGLKFPGEGNPTYGDFLYYSFVIGCASQTADVETVSKAMRRTTLAHGIVSFFF
                     NTVILALTINIGAGLV"
     gene            1147..1533
                     /locus_tag="VKU03_08480"
     CDS             1147..1533
                     /locus_tag="VKU03_08480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612566.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HLJ71338.1"
                     /translation="MAEREGGEEEDRVLRARLEKLSGALEAQRKASQGRREAGGGDQP
                     SDRSGFAMGLAMRAASEFVASIIVGGFIGWLLDAWLGTKPGFLIAFFFLGVAAGIVNL
                     VRATSPSRRRDGRSGEPSARSETRED"
     gene            1562..2311
                     /locus_tag="VKU03_08485"
     CDS             1562..2311
                     /locus_tag="VKU03_08485"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011475214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HLJ71339.1"
                     /translation="MNPIEQFSVTTLVPIHIGHLDLSFTNSSLYMFIAVAIVSALMLI
                     GANGSTGAPGRLQALAEMAYEFMAGMLRSAAGEHAEAFFPFAFCIFMFVLILNLVGTI
                     PYTFSVTSHIVITAALALLVFFTVIIVGIAKHGLHFFKNFVPPGVPIYILPLVVALEV
                     VSFVTRPVSHSVRLFANMLAGHITLNVFGGFVIMLLGAGTFAFRPMAVLPLAMNVGLE
                     ALEVLVAFLQAYVFAMLTCMYLNDALHPGGH"
     gene            2363..2590
                     /locus_tag="VKU03_08490"
     CDS             2363..2590
                     /locus_tag="VKU03_08490"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002714289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit C"
                     /protein_id="HLJ71340.1"
                     /translation="MDPVAAKYIGAGLAAIGTGAAGIGVGTLFGQFLNGALRNPSAVE
                     GQFGRLIFGFAVTEALGIFALLISFLLLFAI"
     gene            2654..3202
                     /locus_tag="VKU03_08495"
     CDS             2654..3202
                     /locus_tag="VKU03_08495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B'"
                     /protein_id="HLJ71341.1"
                     /translation="MAQQPAATQTQMQQEAGGHENFPPFNVHTFPSQILWLAISFGAL
                     YVIMARVALPKLGGVIEARKARIAKDLDDAAALQQKADAAAAAHEKTIAEARGKALAM
                     AQEARDKLAAETAAKRKALEDELSVKLAAADQQIAATRAQAMSNVETIARDAAGAIFE
                     RILGRAPDAQAIAQAVSSVKNA"
     gene            3219..3710
                     /locus_tag="VKU03_08500"
     CDS             3219..3710
                     /locus_tag="VKU03_08500"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015663913.1"
                     /note="Produces ATP from ADP in the presence of a proton
                     gradient across the membrane. Subunit B is part of the
                     membrane proton channel; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP F0F1 synthase subunit B"
                     /protein_id="HLJ71342.1"
                     /translation="MSLFSNAEVFVTIAFIVFLCLLGYIGAHRMVLSALDKRGKDIAE
                     ELGEAAKLRDEALALLASFEKRAAEAEAHAAEIVANAKTEAEALAKETAERLAEFVAR
                     RTKQAEAKIAMAEAQAAADVRAAAADQAAKAAEIVLRKETQGATGADLVAREIAGLKS
                     RLN"
     gene            complement(3746..4150)
                     /locus_tag="VKU03_08505"
     CDS             complement(3746..4150)
                     /locus_tag="VKU03_08505"
                     /inference="COORDINATES: protein motif:HMM:NF018894.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLJ71343.1"
                     /translation="MGSTPDKGRPEERLARRERTLLPARISFGDGGALSTECVVTQLS
                     EIGARVNVPASLAMPDRFDLSIPQRGLTLRARLVWRKDDQAGIAFDKPEPEPPAKPED
                     LQAKVRELEAANAKLRARVAELVEQVRRLTEM"
     gene            complement(4282..5445)
                     /gene="argE"
                     /locus_tag="VKU03_08510"
     CDS             complement(4282..5445)
                     /gene="argE"
                     /locus_tag="VKU03_08510"
                     /EC_number="3.5.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008777 - acetylornithine deacetylase
                     activity [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylornithine deacetylase"
                     /protein_id="HLJ71344.1"
                     /translation="MSETIRAEAIKLLERLVAFDTESDKSNLGLIDFVESWLRQRGVE
                     VLRAPNRAGDKAALLATIGPKIDGGVVLSGHTDVVPVAGQKWTGNPFALRAEGERLYG
                     RGACDMKGFDAIALAMAPHFQAAKLRRPVHILLSYDEETSCLGSLDVIARFGKDLPRP
                     SAVIVGEPTMMTVVDAHKSIATFMTTVTGFEAHSSNPALGANAVAAACEIVAEIGRLA
                     DSYAAEQSHPRFDPPYSTLHVGTIHGGTARNILARQCAFHWEFRGVPGDEAAAALRRV
                     QAYIDRVALPRLTRYVKGPGVETVVEVDIPGLAAEPGSAAETLALKLTRSNQTRAVSF
                     ATEAGQFQTAGLPTIVCGPGSIEQAHKPDEFLSLAQIDAGIGFMIRLAEELSA"
     gene            5525..5917
                     /gene="apaG"
                     /locus_tag="VKU03_08515"
     CDS             5525..5917
                     /gene="apaG"
                     /locus_tag="VKU03_08515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015689150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Co2+/Mg2+ efflux protein ApaG"
                     /protein_id="HLJ71345.1"
                     /translation="MYSAVSNNIRVTVQPEFLPEHSDPDEGRYFWSYTIEIANRGEVT
                     VQLTHRHWQITDATGHCEEVRGPGVVGEQPKLRPGEAFRYTSGCPLSTPSGIMVGVYR
                     MVDESGRAFDVQIPPFSLDSPYVKRVVN"
BASE COUNT         1024 a         1906 c         1935 g         1056 t
ORIGIN      
        1 ggcttaccct catccggcgc ttcgcgccac cttctcccgc aagcgggaga agggattgcc
       61 gccgcggcta cagatcatac gagccccgcg ccgatgttga tggtcagcgc caggatcacg
      121 gtgttgaaga agaacgagac gattccgtgc gccagcgtcg tccgccgcat cgctttcgag
      181 accgtctcga catcggcggt ctgcgaggcg cagccgatca cgaaggaata gtagagaaag
      241 tcgccatagg tcggattgcc ctcgcccgga aatttcaaac cctcgcgcac gcccggcgtc
      301 gacaaatcgt cgtcctcgtc gccgtaatat tcatgggcgt agtggatggc gaagagcaga
      361 tggatgaagg tccaggcgct gagaatggtc gccgtcacca ggccgatatg ggcggccttg
      421 ctccagccgg tcatgtcctt gaccgcgccg agctcggaga cgatcgccgc aagggccgcg
      481 caggcggcga cggtgccgac gaccagcaac acccattgcc gctcgtcctc cagggcgacg
      541 cgcttgcgga tcgattgctc cgtcgcttgg gccatcatcc agccgaccag ggcgatgaac
      601 agcagcgcgc cgatgttcca ggccaggagc gctcgcgtcg cgaagcgcag atcgtgcggc
      661 agcgcgaaat aggcgagaac cgccgccgcg ccgcaaatat agagccgcgg ccgatggcgg
      721 acggcgtcga gaaagcgggc gcgtcgcggc gcgccgccgg cgggcgaggg gatggacatt
      781 taaaaatctc cgcgcgtgac ctgcccgagg ccgttggaag ggtcaagcgt cgcccgcgcc
      841 ttgccgcaaa tcggcgtccc gccgcccgcg ccgcggccgg gcgaagcgcc gggccctcgc
      901 ggaaaacgtt cggccggcgg gaagcttcaa ataggctttc gtgaaacaga tcaggcgctt
      961 actcgctttc tcctgattct tgacaccctc cggcgccgtc aatatggtgc gcgcggcttt
     1021 taaggggcgg ttgacttcaa tccgatttca cttcacggct tttttcgggc cgttcgtccc
     1081 aagctcgtct ctgtagacgt tgagccggat cgacggcgcc ggccgcttta gggcggcgga
     1141 ggcgcgatgg cggaacgcga aggcggcgag gaagaagatc gggttttgcg ggcgaggctt
     1201 gagaagctct caggcgcgct cgaggcgcag cgcaaagcgt cgcaagggcg gcgcgaggcg
     1261 ggcggcggag atcagccctc ggacaggtcc ggcttcgcga tgggtttggc catgcgcgcg
     1321 gccagcgagt tcgtggcgtc gatcatcgtc ggcggcttta tcggctggct gctcgacgcg
     1381 tggttgggca ccaaacccgg cttcctgatc gccttcttct ttctgggcgt ggcggccggg
     1441 atcgtgaact tagtccgcgc gacgagcccg tcgcgccgga gagacggacg ttcgggcgag
     1501 ccgagcgccc ggtcggaaac gagagaggat tagggacgtg gcggcggaag cgggacatgc
     1561 gatcaatccg atcgagcaat tctcggtcac gacgctcgtg ccgatccata tcggccatct
     1621 cgatctgtcc ttcaccaatt cctcgcttta catgttcatc gcggtggcga tcgtctcggc
     1681 gttgatgctg atcggcgcca atggctcgac cggcgccccc gggcgcctgc aggcgctcgc
     1741 cgaaatggcc tacgagttca tggccggcat gttgcgctcg gcggccggcg aacatgccga
     1801 ggcgttcttt cccttcgcct tctgcatttt tatgttcgtg ctgattctca acctcgtcgg
     1861 cacgatcccc tatacgttct cggtgacgag ccatatcgtc atcaccgccg ccttggcgct
     1921 gctggtcttc ttcaccgtca tcatcgtcgg catcgccaag cacgggctgc atttcttcaa
     1981 gaacttcgtg ccgcccggcg tgccgatcta tatcctgccc ttggtcgtgg cgctggaagt
     2041 cgtctcgttc gtcacgcggc cggtcagcca ctccgtccgt ctcttcgcca atatgctggc
     2101 cggccatatc acgctgaacg tcttcggcgg cttcgtcatc atgctgctcg gcgccggcac
     2161 tttcgccttt cggccgatgg cggtcctgcc tctcgcgatg aatgtcggcc tcgaagcgct
     2221 cgaagtcctg gtcgccttcc tgcaggccta tgttttcgcg atgttgacct gcatgtatct
     2281 caacgacgcg cttcaccccg gcggccattg acggcgcctt tttagacaaa caaccaagcc
     2341 atccaagata aaggagcatt cgatggatcc cgttgcagcc aaatatatcg gcgctggcct
     2401 cgccgcgatc ggaaccggcg ccgccggcat cggcgtcggc acgttgttcg gccagttcct
     2461 caacggcgcc ttgcgcaatc catcggcggt ggaaggccag ttcggccggt tgattttcgg
     2521 cttcgcggtg accgaggcgc tcggcatttt cgcgctgctg atctccttcc tcctgctttt
     2581 cgcgatctga tcgaccgctc cggagaaatg acggggagcg gcgacaagcc gttccgccgc
     2641 aagggttcaa gatatggcgc agcaaccagc cgcgacgcaa acgcaaatgc aacaggaggc
     2701 ggggggccac gaaaacttcc cgcctttcaa cgtgcatact tttccatcgc agatcctctg
     2761 gctcgcgatc agcttcggcg cgctctatgt gatcatggcg cgcgtcgcgc tgccgaagct
     2821 cggcggcgtg atcgaggcgc gcaaggcgcg gatcgccaag gatctcgacg acgcggcggc
     2881 tctgcagcaa aaggccgacg ccgcggcggc ggcgcatgaa aagacgatcg ccgaggcgcg
     2941 cggcaaggcg ctcgccatgg cgcaggaggc gcgcgacaag ctcgccgctg aaaccgcggc
     3001 caagcgcaag gcgcttgaag acgaactctc cgtcaagctc gccgccgccg accagcagat
     3061 cgcggcgacg cgcgcgcagg cgatgagcaa tgtcgagacg attgcgcgcg atgcggccgg
     3121 cgcgattttc gagcgcattc tcggccgcgc gccggacgcc caggcgatcg cccaggcggt
     3181 ctcctcggtc aagaacgcgt aacaaggctt aaaagacgat gtcgctgttc tccaacgccg
     3241 aagttttcgt caccatcgcc ttcatcgtct tcctgtgctt gctcggctat atcggcgcgc
     3301 accggatggt cttgtcggcg ctcgataagc gcggcaagga catcgccgag gaattggggg
     3361 aagcggcgaa attgcgcgac gaagcgctcg ctttgctggc gagtttcgaa aagagggccg
     3421 cagaagccga ggcccacgcc gccgaaatcg tcgccaacgc caagaccgag gccgaggcgc
     3481 tcgccaagga aaccgccgag cggcttgccg aattcgtcgc ccgccgcacc aagcaggccg
     3541 aggcgaaaat cgccatggcc gaggcgcaag ccgccgcgga cgtgcgcgcc gccgccgccg
     3601 atcaggccgc caaagcggcc gagatcgttc tgcgcaagga gacgcagggc gcgaccggcg
     3661 ccgatctcgt cgcgcgcgaa atcgccgggc tgaagagccg gctgaattaa ggtcgtcatt
     3721 ccgcttgtgc cggactggcg cgcttttaca tctcagtgag ccggcgcacc tgctcaacca
     3781 attcggcgac acgggcgcgc agtttggcgt tcgccgcctc caattcgcgc actttcgcct
     3841 ggaggtcttc cggcttcgcc ggcggctccg gctcgggttt gtcgaaggcg attccggcct
     3901 gatcgtcctt tcgccagacc agccgcgcgc gaagggtcaa gccgcgctgt ggaatcgaga
     3961 gatcgaagcg atcgggcatg gctaggctgg ccggcacgtt gacgcgggcg ccgatctcgg
     4021 acaattgcgt cacgacgcat tctgtcgaga gcgccccgcc gtcgccgaag gaaattcgcg
     4081 ccggcagcag cgtgcgctcg cggcgggcca gccgctcctc cggccggcct ttgtcgggcg
     4141 tcgagcccat ttaacctaac ctcccgggaa aactcgcaga ttcgaattgg cgcggcgaaa
     4201 gtagcgctcg aatcttgatt aatattaaag accggaggca cgacgaattg tcgcatccgt
     4261 cgtccggcga ggtcggcttt gttaagccga gagctcttcg gctagccgga tcatgaagcc
     4321 gattcccgca tcgatctggg cgaggctgag gaattcgtcc ggcttatgcg cctgctcgat
     4381 cgagccgggg ccgcagacga tggtcggcag gcccgcggtc tggaactggc ccgcctccgt
     4441 ggcgaaggag acggcgcggg tctgattgga gcgcgtcagt ttcaacgcca acgtctcggc
     4501 cgccgatccc ggctcggcgg caaggcccgg aatatcgacc tcgacgaccg tttcgacgcc
     4561 cggccctttg acatagcgcg tcaggcgggg aagcgcgacg cggtcgatat aggcctggac
     4621 tcggcgcagc gcggcggccg cctcgtcgcc gggcacgccg cgaaactccc aatggaacgc
     4681 gcattggcgg gcgagaatgt tgcgcgccgt gccgccatgg atcgttccga catgcaacgt
     4741 cgaatagggc ggatcgaagc gcggatggct ttgctccgcc gcgtaggaat cggcgaggcg
     4801 gccgatctca gcgacgatct cgcacgccgc agcgaccgca ttggcgccca aagcggggtt
     4861 ggacgaatgc gcttcgaagc cggtgacggt cgtcatgaac gtcgcgatgc tcttatgggc
     4921 gtcgacgacc gtcatcatcg tcggctcgcc gacgatcacc gcggatgggc gcggcaggtc
     4981 cttgccgaac cgggcgatga cgtcgagcga gccgaggcaa ctcgtctctt cgtcgtagct
     5041 caacagaata tgcacgggcc gccgtaattt cgcggcctgg aaatgcggcg ccatcgccag
     5101 cgcgatcgcg tcgaagccct tcatgtcgca agcgccgcgg ccatagaggc gctcgccttc
     5161 ggcgcgcaag gcgaagggat tgcccgtcca tttctggccg gcgaccggga cgacgtcggt
     5221 atggccggaa aggacgaccc cgccatcgat cttcgggccg atcgtcgcca gcagggcggc
     5281 cttgtcgccg gcgcgattgg gcgcgcgcaa gacctcgacg ccgcgctggc ggagccagct
     5341 ctcgacgaag tcgatcaagc cgagattgct tttgtcgctc tccgtatcga aggcgacgag
     5401 ccgctcgagc agcttgatcg cttcggccct gatcgtctcg ctcaactttt gcctcctttg
     5461 gagacgccgc ggcgcctctt cagcgggact cgacgcgccg cgctcggcgg ctagactgtg
     5521 gcccatgtat agcgcggtca gcaacaatat ccgggtgact gttcagccgg agttcctgcc
     5581 tgaacattcc gatccggacg aagggcgtta tttctggtcg tatacgatcg agatcgccaa
     5641 tcgcggcgag gtgacggtac aactcacgca tcggcattgg caaatcaccg acgctacggg
     5701 ccattgcgaa gaggtgcgcg gacccggcgt cgtcggcgag cagccgaaac tccggccggg
     5761 cgaggctttc cgctacacgt ccggctgtcc gctaagcacg ccgagcggca tcatggtcgg
     5821 cgtctatcgc atggtcgacg agagcggccg cgctttcgac gtgcagatcc cgcccttctc
     5881 gctcgacagc ccctatgtga agcgggtggt gaattgaggg c
//
LOCUS       DATGJW010000276         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_881446, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000276 DATGJW010000000
VERSION     DATGJW010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_881446"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..1175
                     /locus_tag="VKU03_08520"
     CDS             114..1175
                     /locus_tag="VKU03_08520"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71346.1"
                     /translation="MSEAAPTLSLAAMLQARRVRLKPSGLAGRILLALLLSVICAAAL
                     IALSGHDPLAAFAAIARGAAGSEHQIGASLNRSTPYLVAGVGVALCFRGGVINIGAEG
                     QIAVGGAGAAATALAFSAAPAPIAIVAALLGGALAGAAWAGVATAIHLGRRVHEVLAT
                     LLLNFVALLLVQQLLAGPLGQFGAGFLQSPLMPRSTWLPRLPGFDAHPGFLIAVVAAA
                     ACSFVLWRTPFGFALRVAGSSRLAAAYAGFSLARLTWGAMLAAGALAGLAGGIEVLGV
                     HRRLIEGFSLGFGFKAVTVALLGAMEPLAVVPAALFVGLLESGSLAMQRQIGVPSALV
                     TVIEGLTMLFVLVATVARK"
     gene            1172..2086
                     /locus_tag="VKU03_08525"
     CDS             1172..2086
                     /locus_tag="VKU03_08525"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71347.1"
                     /translation="MIEFLAATIRIATPLALAALGGILSERAGVFAVGLEGMMLAGAF
                     AATVGAWASGEPAIGMLLALIGGAAIGLAVAIVAVRFRADNMVTGLAVNILSLGLTSY
                     LMRIIAGGGRPVNIHLTPLAALPIPGLADIPVLGRLLFTHPPLTYVTALGCVALTLFL
                     GRTQAGLTLRATGENPEAVFASGANPLIVRMLAVVSGGAIAGLGGAVLTLQQVGTFTD
                     GMTSGRGYLALAALIVGRWTPWGAAAACLVFGAAEAFELRLQSFGVPVSSYIIQMVPY
                     LIALAVLAGLGQSSKLPASIGQPLQREH"
BASE COUNT          250 a          726 c          780 g          436 t
ORIGIN      
        1 ctcgcgatca gcgatcgcgt cgcggtcatg gcgagaggag gctttgccgg gatcgtgccg
       61 cggccggacg tggatatgcg gcaaatcggc ctgtggatgg cggggcgggc ggcatgagcg
      121 aagcggcgcc gacattgagc cttgcggcga tgttgcaggc tcgacgggtt cggctgaagc
      181 cgagcggact cgccggccgg attttgctcg ctctgctctt gtcggtgatt tgcgccgccg
      241 cgctgatcgc gctttcgggt cacgatcctt tggcggcgtt cgccgccatt gcgcgaggcg
      301 cggctggatc ggagcatcag atcggcgcct cgctgaaccg ttccacgccc tatctcgtcg
      361 cgggcgtcgg cgtcgcgctc tgtttccgcg gcggcgtcat caatatcggc gcggaggggc
      421 agatcgcggt cggcggcgcc ggcgcggcgg cgaccgcgct cgccttttcg gcggcgccgg
      481 cgccgattgc gatcgttgcg gccctgctcg gcggcgcctt ggccggcgcg gcctgggccg
      541 gcgtcgcaac cgcgatccat ctcggccggc gcgtgcatga agtgctggcg acattgctgc
      601 tcaatttcgt cgcgctcctg ctcgtgcagc aattgctggc cggtccgctc ggccagttcg
      661 gcgccggctt cctccaatct ccccttatgc cccgctccac gtggctgccg agactgcccg
      721 ggttcgacgc ccatccgggc ttcctgatcg ccgtcgtcgc cgccgcggct tgttctttcg
      781 tactctggcg gacgccgttc ggattcgcgt tgcgggttgc gggcagctcc cgcctggccg
      841 cggcctatgc cgggttttcg ttggcgcgcc tgacctgggg cgccatgctc gccgccggcg
      901 cgttggcggg cttggccggc ggcattgagg tgctcggcgt ccatcgccgg ctgatcgagg
      961 gcttctcgct cggcttcggc ttcaaggcgg tgaccgtcgc tctgctcggc gcgatggagc
     1021 cgctcgccgt cgtgcccgcc gctttattcg tcgggcttct cgaatccggt tcgctggcga
     1081 tgcagcggca gatcggcgta ccctcggcgc tggtcaccgt gatcgaaggc ttgacgatgc
     1141 tgtttgtgct ggtggcgacg gtggcgcgca aatgatcgaa tttctcgccg caacgattcg
     1201 gatcgcgacg ccgctcgcat tggccgcttt gggcggaatc ctgtcggagc gcgccggcgt
     1261 cttcgcggtc ggcctcgaag ggatgatgct ggccggcgcg tttgcggcga cggtcggcgc
     1321 ctgggccagc ggcgagccgg caatcggcat gcttttggcg ctaatcggcg gcgcggcgat
     1381 cgggcttgcg gtggcgatcg tcgcagtgcg gtttcgcgcc gacaatatgg tcactgggtt
     1441 ggcggtgaat attctgtcgc tcggcctgac gagctacctg atgcgcatca tcgcaggagg
     1501 cggtcggccg gttaacatcc atctgacgcc gctggccgcc ctgccgattc ctggcctcgc
     1561 cgacataccg gtcctcggcc ggttgctgtt cactcatccg ccgctgacct atgtgacggc
     1621 gctcggctgc gtcgcgctga cgctgttcct cggccgcacg caagcgggac tgacgcttcg
     1681 cgcgaccggc gaaaatccag aggcggtctt cgcctcgggc gcgaaccctc tgatcgtgcg
     1741 gatgctcgcc gtggtgagcg gcggcgcgat cgccggtctc ggcggcgcgg tgctgacgct
     1801 gcagcaggtc ggcacgttca ccgacggcat gaccagcggc cgcggctatc tggcgctcgc
     1861 cgcgctgatc gtcggtcggt ggacgccatg gggcgccgcc gccgcctgcc tggtgttcgg
     1921 cgcggccgaa gcgttcgaac tgcgattgca gagcttcggc gtgccggtca gttcgtacat
     1981 catccagatg gtgccctatc tgatcgcgct cgcggtgctg gcgggcctcg gccaatcctc
     2041 gaagcttcca gcctctatcg ggcagccgtt gcaacgcgaa cattgagggc gccatgaatc
     2101 gtcgaattgc cgagatcgcg gccttgctgc gaagccgaga gctaacggcc gcggcgctgc
     2161 tcgacgattg cctcgcgaga atccgccgca tc
//
LOCUS       DATGJW010000277         7286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_156173, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000277 DATGJW010000000
VERSION     DATGJW010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7286
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156173"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..510)
                     /locus_tag="VKU03_08530"
     CDS             complement(<1..510)
                     /locus_tag="VKU03_08530"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase SLT domain-containing protein"
                     /protein_id="HLJ71348.1"
                     /translation="MTAVLTISNHNAAFAGNENGGSMYFKTSAQPALREGRAAAVEEA
                     TSARRLGAGRGIYAMVTEAAERHGVPVALAHAIIKIESGYNCHARSRYGAVGIGQVLP
                     SSARAEGVSGNLYECRTGLEASMRYLKHAVALYGDSCAAASAYNTGIFARGRCTGYGR
                     RAMRLAQAIK"
     gene            807..1709
                     /gene="pdxY"
                     /locus_tag="VKU03_08535"
     CDS             807..1709
                     /gene="pdxY"
                     /locus_tag="VKU03_08535"
                     /EC_number="2.7.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007558413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008478 - pyridoxal kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009443 - pyridoxal 5'-phosphate salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal kinase PdxY"
                     /protein_id="HLJ71349.1"
                     /translation="MSAEPAETNGKAGERPLSSAILSIQSHVVYGHVGNSAAVFALQR
                     LGREVWPIHTVQFSNHTGYGGWRGRVFAAGDIDECLAGLSERGVLGGCAGLLSGYVGS
                     PEIGAAILRAVGQVTAANPQAIYCCDPVLGDRDRGFYVRDGVADFMRERALPAAAIVT
                     PNHFELDVLAGGEARTLGEARSALARLRAAGPRVALVTSVQLDDTPPHGLDLLACDGE
                     ALWRLRTPKAPVAVNGAGDVVAALFLHHWLERRDAAEALARAASAVYGLVAATEAAGL
                     RELALIAAQDELVNPSRLFAPERV"
     gene            1722..2621
                     /locus_tag="VKU03_08540"
     CDS             1722..2621
                     /locus_tag="VKU03_08540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhzF family phenazine biosynthesis protein"
                     /protein_id="HLJ71350.1"
                     /translation="MRRKFHTLDVFTETALAGNPLAVVLDCEGLDDRRMQAIAREFNL
                     AETVFVLEPRDPVNTARLRIFTPGRELPFAGHPTVGAAVLLAQLRAPELLARQDLRIV
                     VEEGVGDIVCVARHRPNRAPAANFTVPRLPAPVGDAPSVETLARQLGLEPSDIGFGTH
                     RPSVYSAGNPFVFAPVADRAALARARPNANAWGEGGGPGVYLYTRDAERQGSSFRARM
                     FAAGWGVVEDPATGSAAAAFAAVLMRFEKPAEGESVYVIEQGFEMGRPSLISLSVDVE
                     NGALSAAAIGGAVVMITQGTIDA"
     gene            2618..3349
                     /locus_tag="VKU03_08545"
     CDS             2618..3349
                     /locus_tag="VKU03_08545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX hydrolase"
                     /protein_id="HLJ71351.1"
                     /translation="MSGAASRILRVAALDLKLRPRAWAFAEEHAEAILAHWRKRRAER
                     PNLFDGRVLLMGSHEFVKRADGATILAGDYFETDFKAFLAWRDFGFPDRSVCNGFSMA
                     ALQSSDGAYLLGEMADHTANAGAIYFAAGTPDPSDVFGGRIDLYASVVRELAEETGLK
                     VDDLDVAADWIVVDAPPRIACMKPVKAALDAALLKARIESYLAGEERPELKRMHIARS
                     PADIDETRMAQFTVDFLRYAFGVLN"
     gene            complement(3436..3777)
                     /locus_tag="VKU03_08550"
     CDS             complement(3436..3777)
                     /locus_tag="VKU03_08550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71352.1"
                     /translation="MKFPLAIACVALLLAGCNTAQPAATAPPPVAASTDVTPSNFVMP
                     AGSGCAGDIARYRAVQNNDLAMGNVAQSVYNQIKGEIAAAERVCAAGKDREASAMIMA
                     SRKRHGYPAEM"
     gene            complement(3877..4899)
                     /gene="lpxK"
                     /locus_tag="VKU03_08555"
     CDS             complement(3877..4899)
                     /gene="lpxK"
                     /locus_tag="VKU03_08555"
                     /EC_number="2.7.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007560022.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009029 - tetraacyldisaccharide 4'-kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetraacyldisaccharide 4'-kinase"
                     /protein_id="HLJ71353.1"
                     /translation="MKAPRFWAAAPRSAAARALQPLGAAYGALAAWRMARPGRRVAAP
                     VVCIGNFTVGGAGKTPAAIALAKLLAARGENVAFLSRGYGGAARGAPVEVDPKRHSAD
                     EVGDEPLLLARVAPCFVARDRVAAAEAAIAAGAGVLVLDDGLQNPALAKDFSLAMIDG
                     ASGFGNGLCLPAGPLRAPASAQWPHVAIAIVVDAPDRAAIGWRDAGGGPATGARLAPD
                     PTVAAQIKGRDLLAFAGIGRPEKFFATLTALGARLGATRGFPDHHRYGESELDALFDE
                     AARQGLLIVTTEKDFVRLPATYAEKSIALPVTLRFDDEPLIAGLLDEALARRSRRGTA
                     ASDAKN"
     gene            complement(4896..6176)
                     /locus_tag="VKU03_08560"
     CDS             complement(4896..6176)
                     /locus_tag="VKU03_08560"
                     /EC_number="2.4.99.12"
                     /EC_number="2.4.99.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-D-manno-octulosonic acid transferase"
                     /protein_id="HLJ71354.1"
                     /translation="MAPASLIAYRLGARLLAPLSGPLLAYRRARGKEDAARLGERRGR
                     PSRKRPAGSLVWLHGASVGEALSLLPLIERLSQAGQATLITTGTVTSARLLAERLPAG
                     ALHQYAPLDAPQYMRRFIAHWRPDAALIAESELWPNMILEASAADTPLILVNARLSER
                     SYLRWKARAPNFIGALLARVDLALAQSKADAERFSLLGAPRVEFAGNLKFDAPAPPAD
                     SQELAMLSGRIAGRRVWIAASTHEGEEIAIAETHRLLAARFPELLTLIAPRHPSRGAE
                     IAAALHRVGLSCARRSQGAQPGGEAIYICDTMGELGLFYRLAGVVFLGKSLTKEAGGG
                     QNPIEPAKLANAILHGPYVGNFADVYALLDAAGGAEAAHDARGLAEALSALFGDGGRL
                     RAMARAAAQAVESQTGALERTIRAIAPYLVEGKA"
     gene            complement(6176..6886)
                     /locus_tag="VKU03_08565"
     CDS             complement(6176..6886)
                     /locus_tag="VKU03_08565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLJ71355.1"
                     /translation="MLKRLGKSKLAHEALGLLLAAYIRLVRRTNRFDQPDLEALVDGK
                     LPVIVAMWHGQHFMIPFARPKTMPRIAALISRHGDAGANAAALRWLGVIPIRGSGGRA
                     EQMKKKGGAPAMREMLRALGEGASVAMTADIPKRARVAGMGIVTLGRLSGRPIAPVAV
                     VTSRRFDFKSWDRASIGKPFGRGAIVVGEFIHVPPDADEATMEALRQKVEKGLDQAHA
                     QAYAKVGAADPGAGLRRD"
     gene            complement(6890..7162)
                     /locus_tag="VKU03_08570"
     CDS             complement(6890..7162)
                     /locus_tag="VKU03_08570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015598992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4170 domain-containing protein"
                     /protein_id="HLJ71356.1"
                     /translation="MTEPGRDEVASAESPQLLHLVFGGELKNLETYEFRDPSKIDVVG
                     IFPDYASAERAWRGKAQMTVDSAQTRYFIVHLHRFLDPSGLARKKP"
BASE COUNT         1121 a         2430 c         2542 g         1193 t
ORIGIN      
        1 ttttatcgct tgcgccagcc gcatcgcgcg gcggccatag ccggtgcagc ggccgcgagc
       61 gaaaatgccg gtattgtagg cggatgcggc ggcgcaactg tcgccgtaaa gcgcgaccgc
      121 gtgcttcaga tagcgcatcg aggcctccag gccggtgcgg cactcataga gattgcccga
      181 gacgccttcg gcgcgcgcgc tggacggcag cacttggccg atgccgaccg cgccgtagcg
      241 cgagcgggca tggcaattgt agccgctctc gatcttgatg atcgcatgag caagggcgac
      301 cggcacgccg tgccgctcgg cggcttccgt gaccatggcg taaattcccc gcccggcgcc
      361 gaggcggcgc gccgaggtcg cttcctccac cgccgctgcg cggccctcgc gcaaagccgg
      421 ctgggcggac gtcttgaaat acatcgagcc gccgttttca ttgccggcaa aggcggcgtt
      481 gtgattggat atcgttagaa ccgccgtcaa tgcggcggcc aagcaaatgg aatttaagga
      541 ctttgtcgaa ttgtggcgaa gcttcgaagc gtagacgtga gcgatgctcg tcatcattcc
      601 ccgctgttac agttgtgtcg atacgatggc gaaggatgga cttcgaattt gttccatctt
      661 cttggtcacg ttctggtgcg cggcgggcaa gagctatcca aatgtgagca taatatgaca
      721 ggacatggag ccattactgc cggcgacgta aagcattatt ttcaaaggga taaaagtttg
      781 gcccagatgg cggcgcggcc gcgaccatga gcgccgagcc ggccgaaacg aacggaaaag
      841 cgggcgaaag gcctctctcc tccgccattt tgtcgatcca gtcgcatgtc gtttacggcc
      901 atgtcggcaa ttcggcggcc gtgttcgcgc tgcagcggct cggccgagag gtttggccga
      961 tccacaccgt gcaattctcc aatcacaccg gctatggcgg ctggcgcggc cgggttttcg
     1021 ccgccggcga catcgacgaa tgcctcgctg gtctttccga acgcggcgtg ctcggcggct
     1081 gcgccggcct gctctccggc tatgtcggct cgccggaaat cggcgcggcg attctccgcg
     1141 ccgtcgggca ggtcacagcc gccaatccgc aggcgatcta ttgctgcgat ccggtgctcg
     1201 gcgacaggga ccgcggcttc tatgtgcgcg acggcgtcgc cgatttcatg cgcgagcgcg
     1261 cgcttcccgc cgcggcgatc gtcacgccca atcatttcga gctcgatgtc ctggccggcg
     1321 gcgaagcgcg cacgctcggc gaggcgcgca gcgccttggc gaggctgcgc gcggccggtc
     1381 cgcgcgtcgc gctcgtcacc tcggtccaac tcgacgatac gccgccgcac ggcctcgatc
     1441 ttctcgcttg cgacggcgag gcgctttggc gcctgcgcac gcctaaagcg ccggtcgcgg
     1501 tcaatggcgc cggcgacgtc gtcgcggcgc tctttctcca tcactggctg gaacggcgcg
     1561 acgccgccga ggcgctcgca agagcggcct ccgccgtcta tggtctcgtc gccgcgacgg
     1621 aggcggcggg cttgcgcgag ctcgcgctga tcgcggcgca ggacgaactc gtcaacccgt
     1681 cgcggctctt cgcgccggag cgcgtgtgag aaaggaaatt gatgcggcgg aaattccaca
     1741 cgctcgacgt cttcacggaa accgcgctgg ccggcaatcc gctcgccgtg gtgctcgatt
     1801 gcgagggcct cgacgatcgg cgcatgcagg cgatcgcgcg cgagttcaat ctcgccgaga
     1861 cggtcttcgt cctggagccg cgcgatcccg tgaacacggc gcgcctgcgc atcttcacgc
     1921 cggggcgcga attgcctttc gccggccatc cgaccgtcgg cgccgcggtg ctgctggcgc
     1981 aattgcgcgc gccggaattg ttggcgcggc aggatctgcg catcgtcgtc gaggaaggcg
     2041 tcggcgatat tgtctgcgtg gcgcggcatc gccccaatcg ggcgccagcc gccaatttca
     2101 ccgttccgcg cctgccggcg ccggtcggcg acgctccttc cgtcgagacg ctcgcacgcc
     2161 agctcggtct tgagccgagc gacatcggct tcgggactca ccggccgagc gtctattccg
     2221 cgggcaatcc tttcgtcttc gcgccggtcg ccgatcgcgc cgcgctcgcc agggcgcggc
     2281 ccaacgcgaa cgcctggggc gagggcggcg gccccggcgt ctatctttat acgcgcgatg
     2341 cggagcgaca gggctcatcg ttccgcgcac ggatgttcgc ggcgggttgg ggcgtggtcg
     2401 aggacccggc gaccggctcg gccgccgcgg ccttcgccgc cgtcctgatg cgcttcgaaa
     2461 agccggccga gggcgagagc gtctacgtca tcgagcaggg cttcgaaatg ggccggccga
     2521 gcctgatctc gctcagcgtc gacgtcgaga acggcgcgct gagcgcagcg gcgatcggcg
     2581 gcgccgtggt gatgatcacg caaggtacga tcgacgcatg agcggcgcgg caagccgcat
     2641 tcttcgagtc gcggcgctgg accttaagct tcgtccgcgc gcttgggcct tcgccgaaga
     2701 gcacgccgag gcgattctcg ctcattggcg gaagcgccgg gccgagcggc ccaacctctt
     2761 cgacggccgc gttctgctga tgggatcgca cgaattcgtc aagcgcgccg acggcgcgac
     2821 gattctcgcc ggcgactatt tcgagaccga tttcaaggcg tttctcgcct ggcgggattt
     2881 cggctttccc gatcgcagcg tctgcaacgg gttctcgatg gcggcgctgc aatcgtccga
     2941 cggcgcctat ctgctcggcg aaatggccga tcacaccgcc aatgccggcg cgatctattt
     3001 cgccgccggc acgccggacc cgagcgacgt gttcggcggc cgcatcgacc tctatgcaag
     3061 cgtggtgcgc gaattggcgg aggagaccgg cctgaaagtc gacgatctcg atgtggcggc
     3121 ggattggatc gtcgtcgacg cgccgccgcg catcgcttgc atgaagccgg tcaaggcggc
     3181 tctcgacgcc gctttgctca aggcgcggat cgaatcttat cttgcgggcg aagaaaggcc
     3241 ggagctcaag cgcatgcata tcgcgcgctc gcccgccgat attgatgaaa cgcgcatggc
     3301 gcagttcacc gtcgacttcc tgcgctacgc ctttggcgta ctgaactgaa cgtcattgcg
     3361 acgagcacag cgacgaagca atccattcgc aagcctgcgc ccttcgaggg attgcttcgc
     3421 ttgcagcgac gtatgtcaca tctccgccgg atagccgtgc cgcttacgcg aggccatgat
     3481 catggcgctc gcctcgcggt ccttgccggc cgcgcagacc cgctcggcgg cggcgatttc
     3541 gcccttaatc tgattgtaga cggattgtgc gacattgccc atggcgaggt cattgttttg
     3601 gacggcgcgg tagcgggcga tgtcgccggc gcagcctgag ccggcgggca tgacgaaatt
     3661 ggacggcgtc acatcggttg aggcggcgac ggggggcggc gccgtcgcag ccggctgggc
     3721 cgtgttgcag ccggcgagca gcagggcgac gcaggcgatt gcgaggggaa atttcatcgg
     3781 gcgcctcctt atttttgaga atcttgcttt gctttaagaa ggccgcgcgg cgcggccggt
     3841 caagcaaatt tcgtcagcgc gccttattga cgcaaatcaa ttcttggcgt ccgaagccgc
     3901 cgtcccacgc cgcgagcgcc gcgccagagc ttcatccagc aagccggcga tcaggggctc
     3961 gtcgtcgaag cgcagcgtca ccggcagcgc aatggatttt tccgcatagg tcgccggcaa
     4021 gcgtacgaaa tccttctcgg tcgtgacgat gagcaggcct tggcgcgccg cttcgtcgaa
     4081 cagcgcgtcg agctcgcttt cgccatagcg atgatgatcg ggaaagccgc gcgtcgcgcc
     4141 gagccgcgcg cccagcgccg tcagagtcgc gaagaacttc tccggtcggc cgatgccggc
     4201 gaaggcgaga aggtcgcggc ctttaatctg cgcggcgacc gtcggatcgg gcgcgagccg
     4261 cgcgccggtt gctggcccgc ccccggcgtc ccgccagccg atggcggcgc ggtcgggcgc
     4321 atcgaccaca atcgcgatcg caacatgcgg ccattgcgcg ctcgccggcg cgcgcagcgg
     4381 tcccgccggc aggcagagcc cgttgccgaa gccggaggcg ccgtcgatca tcgccaggct
     4441 gaaatccttg gcgagggccg gattctgcaa gccgtcgtcg aggacgagaa cgccggcgcc
     4501 ggcggcgatc gccgcctcgg ccgcggcgac gcgatcgcgc gcgacgaagc agggcgcaac
     4561 gcgcgccagc agcagcggct cgtcgccgac ttcgtcagcc gaatgccgct tcggatcgac
     4621 ctcgaccggc gcgccgcgcg ccgccccgcc atagccgcgc gacaagaaag cgacgttctc
     4681 gccgcgcgcc gccagcagct tggcgagcgc gatcgcggcc ggcgtcttgc cggcgccgcc
     4741 gacggtgaaa ttgccgatgc aaacgaccgg cgcggcgacg cgccggcccg gccgcgccat
     4801 gcgccaggcg gcgagcgcgc catacgcggc gccgagcggc tgcaaggcgc gggcggcggc
     4861 gctgcgcggc gccgcagccc aaaagcgcgg cgccttcacg ccttgccttc gacgagatag
     4921 ggcgcgatcg cgcggatcgt gcgctcgagc gcgccggtct ggctttcgac cgcctgggcg
     4981 gcggcgcggg ccatggcccg caatcggccg ccgtcgccga agagcgcgct caacgcctcg
     5041 gcgaggccgc gcgcgtcgtg cgccgcttcg gcgccgcccg cagcgtcgag cagcgcgtag
     5101 acgtcggcga aattgccgac atacgggcca tgcagaatgg cgttggcgag cttggccggc
     5161 tcgatcggat tttggccgcc gcccgcctcc ttggtcagcg acttgccgag gaagacgacg
     5221 ccggccaggc gatagaacag gccgagctcg cccatcgtgt cgcagatata gatcgcttcg
     5281 ccgccgggct gggcgccttg cgagcggcgc gcgcagctca atccaacacg gtgcagcgcc
     5341 gctgcgatct cggcgccgcg gcttggatgg cgcggcgcga tcagcgtcag caattcggga
     5401 aagcgcgccg cgagcaggcg atgggtttcg gcgatcgcga tctcttcgcc ttcatgggtc
     5461 gaggcggcga tccacaccct ccgtccggcg atgcggccgg acagcatggc cagctcctgc
     5521 gaatcggcag gcggcgccgg cgcgtcgaat ttgagattgc cggcgaattc gacgcgcggc
     5581 gcgccgagca ggctgaaccg ctcggcgtcg gccttgctct gggcgagggc gaggtcgacc
     5641 cgcgcgagca gcgcgccgat gaaattcggc gcccgcgcct tccagcgcag gtacgatcgc
     5701 tcgctgagcc gggcgttgac caggatgagc ggcgtgtcgg cggcgctcgc ttcgagaatc
     5761 atgttcggcc acagttccga ttcggcgatc aaagccgcgt cggggcgcca atgggcgatg
     5821 aaccggcgca tatattgcgg cgcgtcgagc ggcgcatatt gatgcagcgc gccggccggc
     5881 aggcgctccg cgagaagccg cgccgacgtc accgtgccgg tggtgatcag cgtcgcctgg
     5941 ccggcttgcg acaggcgctc gatcagcggc aggagcgaca aggcttcgcc gacgctcgcg
     6001 ccatgcagcc atacgagcga ccctgccggc cgcttgcggc tgggacggcc gcggcgctcg
     6061 ccgagccgcg ctgcgtcctc cttgccgcgg gcgcggcgat aggccagcaa ggggccgctg
     6121 agcggcgcca ggaggcgcgc gcccaaccgg taggcgatga ggctggctgg cgccatcaat
     6181 ctcgtctcag gccggcgccg ggatcggcgg cgccgacctt cgcataggcc tgggcgtgag
     6241 cctggtcgag gcccttctca accttttgcc gcagcgcctc catcgtcgcc tcgtccgcgt
     6301 cgggcgggac gtggatgaat tcgccgacga cgattgcgcc gcggccgaag ggtttgccga
     6361 tgctggcgcg gtcccaggac ttgaaatcga agcgccggct ggtgacgacg gcgaccggcg
     6421 cgatcggacg gccggaaagg cggcccaacg tgacgatccc catgccggcg acgcgcgcgc
     6481 gcttcgggat gtcggcggtc atggcgacgg acgcgccctc gcccagcgcc cgcagcattt
     6541 cgcgcatcgc cggcgcgccg ccttttttct tcatctgctc ggcgcggccg cccgagccgc
     6601 ggatcgggat gaccccgagc cagcgcaaag ccgccgcatt ggccccggcg tcgccgtggc
     6661 gcgagatcag cgcggcgata cgcggcatgg tcttcggcct cgcgaaggga atcatgaagt
     6721 gctgaccgtg ccacatcgcg acgatcaccg gcaatttgcc gtcgaccagc gcttcgagat
     6781 cgggctggtc gaagcgattg gtgcgccgga cgagacggat gtaggcggcg agcaggaggc
     6841 ccagcgcttc atgcgcgagc ttggacttgc ccaggcgttt gagcatgggt cagggctttt
     6901 tgcgcgctag gccggaaggg tcgagaaagc gatggagatg gacgatgaaa tagcgcgtct
     6961 gagcgctgtc gacggtcatc tgcgccttgc cgcgccaagc gcgctccgcg ctggcgtagt
     7021 cgggaaagat gccgaccacg tcgattttcg acggatcgcg aaattcgtac gtttcgagat
     7081 ttttcagctc gccgccgaag acgagatgca ggagctgcgg cgattccgcg ctcgcgacct
     7141 cgtctcgtcc gggttccgtc atgacaggct ccttgaaggg ttggacggcg cggctcaggc
     7201 gggggaggcc aggcttgcgc gcccggtcca agacgcgccg gcggccgcca gcgcctgccc
     7261 gatcagcgct cgcggcgcgg cgaaca
//
LOCUS       DATGJW010000278         5485 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_882632, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000278 DATGJW010000000
VERSION     DATGJW010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5485)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5485)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5485
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_882632"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..199
                     /locus_tag="VKU03_08575"
     CDS             <1..199
                     /locus_tag="VKU03_08575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175216.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLJ71357.1"
                     /translation="PDKTRAGEPIDEIIDGAIFETFDSMPRPKRRDPDVISGAIERAV
                     RNAVNMVWSKKPSVHVLVVEV"
     gene            295..699
                     /gene="mce"
                     /locus_tag="VKU03_08580"
     CDS             295..699
                     /gene="mce"
                     /locus_tag="VKU03_08580"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HLJ71358.1"
                     /translation="MIGRLNHVAIAVRDIAAASAHYRAALGATVSPPLPQLEHGVTVA
                     FVELPNTKIELLEPLGADSPIAKFLEKNPDGGIHHLCFEVADILAARDQLIGGGARIL
                     GGGNPKVGAHGKPVLFLHPKDFNGTLIELEQA"
     gene            complement(696..2396)
                     /locus_tag="VKU03_08585"
     CDS             complement(696..2396)
                     /locus_tag="VKU03_08585"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ71359.1"
                     /translation="MNAQAEEAMERKLRLTTGLILFAYAASHFLGHATGIFGLATLET
                     VGRGIILAPWRTPLGRTALFASLLIHGGLGLYALYRRRTLRMPALEAWQLCLGLTIPF
                     LLAPHILTVRIAADIFKLDDSYQDVLYRVWIAGRRLFNDLSLLALVWTHGCLGLNFWL
                     KRYEFYRWTRDYFLAVAVAVPFVAALGLVNAGWDATTMARQDPSFAAAHGPPIAGSPR
                     AASEQTLNRWAGDLQRLWLVLIAATLLARFARDRLERGAKGVRVTYPDGRVVVAPRGF
                     SVLETSRRAGVAHASACGGRGRCSTCRIQIVAGAEDAPEPSPAERDTLERVKAPPGVR
                     LACQLRPVADLSVIPLLAAAQLSSRAGARETRELLVTAVFIDLRDSTKLAAGRMPFDA
                     LYIVDRYVQAVTESVEAHGGAVTTVAGDGVMSIFGARTDPPSGARGALRAVGAIWDAI
                     EALSRDFAADLDRPLGFGIGVHTGLAAVTATTIGGKSSLQFIGDAGNIASRLEAATKI
                     LSGVCVVSEAVFKVARAEIPSDLAREELSVRGLEGRKLPVAIVRSREEADFAAAPAKI
                     "
     gene            complement(2456..3349)
                     /locus_tag="VKU03_08590"
     CDS             complement(2456..3349)
                     /locus_tag="VKU03_08590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018097700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ71360.1"
                     /translation="MSPAEPNWDLYRTFLAVMEEGSLSAAARALGLAQPTVGRHVEAL
                     EQALGFALFTRSQHGLAPTEGALNLKPYAEALASTAAALLRVASSQGAGARGAVRVTA
                     SEVIGAEVLPAILGPLREAHPELAIELVLSNRIENLLTREADIAVRMVRPDQDALVAR
                     HIGAIPLGLFARSDYVQRHGAPQSLADLAEHVVIGFDRDASSVRAFEAQFGKIDRNLF
                     ALRTDNQLAHLAAIRAGFGVGVCQVGLAKRDPDLIRLLSDQFEVALDTWLAMHEDLKE
                     TPRCRVVFDALADGLTEYVKR"
     gene            3441..4445
                     /locus_tag="VKU03_08595"
     CDS             3441..4445
                     /locus_tag="VKU03_08595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016554666.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLJ71361.1"
                     /translation="MTQEKTALILGATGGIGGETARALKARGWRIRALHRKGGAAGRS
                     GDGLDWVRGDAMNEGDVVAAAKGVSIIVHAVNPPGYRNWGKLVLPMLDSTIAAAQASG
                     ARIVFPGAVYNFGPDAWPALREDSPQNPRTRKGAIRAEMERRLKAAAASGARALIVRA
                     GDFFGPRGTGNSWFGAALVRPGKTPSSVPYPGREGVGHQWAYLPDVAETMARLIDDED
                     RLAAFEVFHMDGHWDPDGTAMVRAIGRACGKPNIKATAFPWWAVRLTSPFVTLFREML
                     EMRYLWREPLRMDNRRLVAFLGEEPHTPLDEAVRETLNGLGCLPNGVAPAPAAAIRAT
                     "
     gene            4592..>5485
                     /locus_tag="VKU03_08600"
     CDS             4592..>5485
                     /locus_tag="VKU03_08600"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HLJ71362.1"
                     /translation="MALLENAPLPEGLAFDDVLLRPGYSAHMPSEVDTTARLTRSIKL
                     NVPILSAAMDTVTEARLAVAMAQAGGIGVIHRNLSPEDQAEEVRKVKRYESGMVVDPI
                     TIFPDATLAEALDLMRRSAISGIPVVERGANGKAAKLVGILTNRDVRFADDPRQPVGE
                     LMTRKVVTVREGVGQDEARRLLHQHRIEKLVVIDEDERCVGLITVKDIEKATLHPNAA
                     KDGEGRLRAAAATTVGDKGYERAELLMAAGVDCLVVDTAHGHSEQVLAQVTRIKRATN
                     KVAVIAGNVATRDGAQALIDAG"
BASE COUNT          963 a         1770 c         1910 g          842 t
ORIGIN      
        1 gcccgacaag actcgcgccg gcgagccgat cgacgagatc atcgacggcg ccattttcga
       61 gacgttcgat tccatgccgc ggccgaagcg ccgcgacccc gacgtgatat ccggcgcgat
      121 cgaacgcgcg gtgcgcaacg cggtgaacat ggtctggagc aagaagccga gcgtccacgt
      181 tctggtcgtc gaggtatagg ctggctcttg accgagaaac gcggcggcgt accctcagcc
      241 cgaagggccg gatgaggtaa gcgccggcgc tggtcattcg cggttggaga ttcaatgatc
      301 ggccggttga accacgtcgc gatcgcggtc agagatattg ccgcagcgag cgcgcactat
      361 cgcgcggcgc tcggcgcgac cgtctcgccg cccttgccgc agcttgagca cggcgtcacc
      421 gtcgcgttcg tcgaattgcc caatacgaaa attgaactgc tggagccgct cggcgccgat
      481 tcgccgatcg cgaaattcct ggagaagaat ccggacggcg gcattcatca cctctgtttc
      541 gaggtcgccg atattttggc cgcgcgggat caactcatcg gcggcggcgc gcgcattctc
      601 ggcggcggca atccgaaagt cggcgctcat ggcaagccgg tcctgttcct gcatccgaaa
      661 gatttcaacg gaacgctgat cgagctggag caggcttaaa ttttcgctgg cgcggcggcg
      721 aaatcggctt cttcgcgcga gcgaacgatc gccaccggca gcttgcggcc ttcgaggccg
      781 cgaacgctca actcctctct cgcgaggtcg ctcggtattt cagcgcgcgc gaccttgaac
      841 accgcttccg agacgacgca gacgccgctc agaatcttgg tcgccgcctc cagccgcgag
      901 gcgatattgc cggcgtcgcc gatgaattgc agcgaagatt tgccgccgat cgtcgtcgcg
      961 gtcactgcgg caaggccggt atggacgccg atgccgaagc cgagcggccg gtcgagatcg
     1021 gcggcgaaat cgcgcgacag cgcttcgatt gcgtcccaga tcgcgccgac cgccctgagc
     1081 gcgccgcgcg cgccggaagg cggatcggtc cgcgcgccga aaatgctcat cacgccgtcg
     1141 ccggcgacgg tcgtcaccgc gccgccatgc gcctcgaccg actcggtcac cgcttgtacg
     1201 tagcgatcga cgatatagag cgcgtcgaaa ggcatgcggc cggcggcgag cttggtcgaa
     1261 tcgcgcagat cgatgaaaac cgccgtcacc agaagctcgc gcgtttcgcg cgcgccggcg
     1321 cgcgaagaaa gttgcgcggc ggcgaggagg ggaatgacgg aaaggtcggc gaccggccgc
     1381 aattggcagg cgagccgcac gccgggcggc gccttgacgc gttcgagcgt gtcgcgctcg
     1441 gccggcgaag gctcaggcgc gtcctcggcg ccggcgacga tctggatgcg gcaggtcgag
     1501 cagcggccgc ggccgccgca ggccgaggcg tgcgcgacgc ccgctcggcg gcttgtctcc
     1561 agcaccgaga agccgcgcgg cgcaacgacc actcggccat ctggataggt cacgcggacg
     1621 cccttggcgc cgcgttcgag gcggtcgcgg gcgaatcggg cgagcagggt ggcggcgatc
     1681 aggacgagcc aaagccgttg caggtcgccg gcccagcgat tgagcgtctg ttcgctggcg
     1741 gcgcgcggcg atcccgcgat cggcggtcca tgcgcggcgg cgaaggacgg gtcctgcctg
     1801 gccatggtcg tcgcatccca tccggcgttg accaggccga gcgccgcgac gaacggaacg
     1861 gcgacggcga cggcgagaaa ataatcgcgc gtccagcgat agaattcgta ccttttcagc
     1921 cagaaattca gcccgagaca gccatgcgtc cagaccagag cgaggagcga aaggtcgttg
     1981 aacagccggc ggccggcgat ccaaacgcga tagaggacgt cctggtagct gtcgtcgagc
     2041 ttgaaaatat cggcggcgat ccggacggtg aggatatgcg gcgccagcag gaacgggatg
     2101 gtcaagccga ggcataattg ccaggcttcg agcgccggca tccgcaaggt gcggcgccgg
     2161 tagagggcat agaggccgag cccgccatga atgagcagcg aggcgaagag cgcggtccgg
     2221 ccgagcggcg tgcgccaggg cgcaagaatg attccgcgcc cgaccgtctc cagcgtcgcc
     2281 aggccgaaaa tccccgtcgc atggcccaga aagtggctgg ccgcataggc gaagagaatg
     2341 aggcccgtcg tcagtctgag cttgcgctcc atcgcttctt ccgcctgggc gttcacggcc
     2401 gaagccgtct ctatagcagg agggacgccg tcctccgcct acagaaaaga ccgatctatc
     2461 gcttcacata ttccgtgagg ccgtccgcca aagcgtcgaa gaccacccgg catcgcggcg
     2521 tctccttcag gtcctcgtgc atggcgagcc acgtatcgag cgcgacctcg aattgatcgc
     2581 tcaagaggcg gatcaagtcc ggatcgcgtt tcgccaggcc gacttggcag acgccgacgc
     2641 caaatccggc gcgaatcgcg gcgaggtggg cgagctgatt gtcggtgcgc aaagcgaaaa
     2701 gattgcggtc gatcttgccg aattgcgcct cgaaagcgcg cacggacgaa gcgtcgcgat
     2761 caaacccgat cactacgtgc tcggcgagat cggcgagaga ttgcggcgcg ccgtggcgct
     2821 gcacataatc gctgcgggcg aagaggccga gggggatcgc gccaatatgc cgggcgacca
     2881 gcgcgtcctg atcgggcctc accatcctca ccgcgatatc ggcctcgcgc gtgagcaagt
     2941 tctcgatccg gttggagagg acgagttcga tcgcaagctc gggatgcgcc tcgcgcaacg
     3001 gcccgagaat ggcgggcaac acctcggcgc cgatcacctc gctggcggtc acgcggacgg
     3061 cgccgcgcgc gccggcgccc tggctcgaag cgaccctgag cagggccgcc gcggtcgacg
     3121 ccagcgcttc ggcatacggc ttcaaattca gcgccccttc ggtcggcgcg aggccgtgct
     3181 gcgagcgggt gaagagggcg aagccgagcg cctgctccag cgcctcgaca tgccggccga
     3241 cggtgggctg agcgaggccg agagcgcgcg ccgcggccga taacgaccct tcctccatga
     3301 cggcgagaaa ggtgcggtag agatcccaat tcggttcggc tggagacatg cggaaatgta
     3361 tatcgtcgcc acggatttgg ccaatttagt ttagccttcg gcgggggcat atcggcgtcg
     3421 tcaccaacga ggagccgatc atgacacagg aaaaaaccgc cttgatcctt ggcgcgacgg
     3481 gcggaatcgg cggtgaaacg gcgcgcgctc tgaaggcgcg cggctggcgc atccgcgcct
     3541 tgcatcgcaa gggcggagcg gccggccgtt ccggcgacgg tctcgactgg gtccgaggcg
     3601 acgcaatgaa tgagggcgac gtcgtcgcgg cggccaaggg cgtctcgatc atcgtccacg
     3661 ccgtcaatcc gccgggttat cgaaattggg gcaagctcgt tctgccgatg ctcgattcga
     3721 ccatcgccgc ggcccaagcg agcggcgcgc gcatcgtctt tcccggcgcg gtctataatt
     3781 tcggacccga cgcctggccg gccctgcgcg aggactcgcc gcaaaatccg cggacgcgca
     3841 aaggcgcgat tcgggccgag atggagcgcc ggctcaaggc cgccgcggct tcgggcgcgc
     3901 gtgcgctcat cgtgcgcgcc ggcgatttct tcggcccgcg cggaaccggc aatagctggt
     3961 tcggcgccgc gctggtcagg cccggcaaaa cgccgagctc tgttccctat ccgggccgcg
     4021 aaggcgtcgg ccatcaatgg gcctatctgc cggacgtcgc cgagacaatg gctcggctga
     4081 ttgacgacga ggaccggctc gccgctttcg aagtttttca catggacggc cattgggacc
     4141 cggacgggac ggcgatggtc cgagcgatcg gtcgcgcctg cggcaagccg aacatcaagg
     4201 cgacggcctt tccgtggtgg gcggtcaggc tgacgtcgcc ttttgtcacg ctgttccgcg
     4261 agatgctgga gatgcgctat ctctggcgcg agcccttgcg catggacaat cgccgtctcg
     4321 tcgcgtttct cggcgaggag ccgcatacgc cgctggacga ggcggttcgc gagacgctga
     4381 acggcctcgg ttgtttgccg aacggcgtcg ctccggcgcc cgctgcggcg attcgcgcga
     4441 cctgatcgag gcgcgaggct ttcgcgcgcg ccgcgctttg cgcgcgcggc gacgcatgtt
     4501 aagagccgtg gcctcgcggc gcgcgctttg ctttgaaagc gccgtcgagc gatcgcggtt
     4561 ttcacgctgc gtcgcccctc aaggagccgc catggccttg ctcgaaaacg cgcccttgcc
     4621 ggagggtctg gccttcgacg acgtgctgct gcgcccaggg tacagcgcgc atatgccgag
     4681 cgaggtcgac acgacggcgc ggctgacgcg gtcgatcaag ctcaacgtgc cgatcctctc
     4741 ggcggcgatg gacacggtga ccgaagcgcg gctggcggtc gcgatggccc aggccggcgg
     4801 gatcggcgtc atccatcgca atctctcgcc cgaggaccag gccgaggagg tgcgcaaggt
     4861 gaagcgctac gagagcggca tggtcgtcga cccaatcacg attttcccgg atgcgacctt
     4921 ggccgaggcg ctcgacctga tgcggcgcag cgcgatttcc ggcatcccgg tggtcgagcg
     4981 cggcgccaac ggcaaggcgg ccaagctcgt cggcatcctg accaatcgcg acgtccgttt
     5041 cgccgacgat ccccgccagc cggtcggcga gctgatgacc cggaaggtcg tcaccgtgcg
     5101 cgagggcgtc ggccaagacg aagcgcgccg gctgcttcat cagcaccgca tcgaaaagct
     5161 cgtcgtgatc gatgaggacg agcgctgcgt cgggctgatc accgtcaagg atatcgagaa
     5221 ggcgacgctc catcccaacg ccgccaagga cggggagggg cgcctgcgcg ccgccgccgc
     5281 gacgacggtc ggcgacaagg gttacgagcg ggcggagttg ctgatggcgg ccggcgtcga
     5341 ttgcctcgtc gtcgacacgg cgcatggcca ttccgagcag gtgctggcgc aagtgacgcg
     5401 gatcaagcgg gcgacgaaca aggtcgcggt gatcgccggc aacgtcgcaa cgagagacgg
     5461 cgcgcaggct ttgatcgacg ccggc
//
LOCUS       DATGJW010000279         5999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_365708, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000279 DATGJW010000000
VERSION     DATGJW010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5999
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_365708"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..307
                     /locus_tag="VKU03_08605"
     CDS             <1..307
                     /locus_tag="VKU03_08605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ornithine decarboxylase"
                     /protein_id="HLJ71363.1"
                     /translation="DDDKLRWVYLDIGKFNGLAETMDEMIRYPIKTPNDEDAMAPCVI
                     AGPTCDSVDVLYEKEPYLLPVSLEIGAKVLIEGTGAYTTTYSAVGFNGFQPLKSYVI"
     gene            448..1050
                     /locus_tag="VKU03_08610"
     CDS             448..1050
                     /locus_tag="VKU03_08610"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HLJ71364.1"
                     /translation="MTLMFDPTRLSACALPPDASARLRPIVIENERPADGPAREALLD
                     EAFGEERFAKTCQRLRDGRATAEGLALVARDGEALVGTLCCWNVTAGDRPALLLGPVA
                     VARSHRSIGIGSALMREALLRAAACGHKAVLLVGDAPYYCRFGFAASLTRGLDLPGPV
                     ERARFLGFEITPGALKGSKGMVTAADVPACKRASSLRRAA"
     gene            1414..2130
                     /locus_tag="VKU03_08615"
     CDS             1414..2130
                     /locus_tag="VKU03_08615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71365.1"
                     /translation="MTGASSGIGEATARALAGAGAKTVIAARRAERLDALAKAIEKNG
                     GEALALAADASLESEARRMLGDVEKSYGRLDILVNNAGVMLLSPVEEAEIGDFRRMIE
                     INLFGLMTLSRLALPIMKRGGAGHFVNISSVAGRIANPGAGAYAATKFGVVAFSESLR
                     REVYKDKIRVTVIEPGMVATELPDHITNPAMRAGLEQRKAAMEPLEAEDIAAAVLYAV
                     TQPPRVNVNEILIRPTLQER"
     gene            complement(2240..2575)
                     /locus_tag="VKU03_08620"
     CDS             complement(2240..2575)
                     /locus_tag="VKU03_08620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015597849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcQ/YjbR family DNA-binding protein"
                     /protein_id="HLJ71366.1"
                     /translation="MATGEDLRRIALSLAGVSEAPHFDRLAFRAARIFATLAADRQSA
                     NIRFAPDEQEFKCLIAPQAFAPLKGGWGAAGWTRAELSALDEAELRVALEAAWRHAQP
                     KPKSARKRR"
     gene            complement(2860..4152)
                     /gene="eno"
                     /locus_tag="VKU03_08625"
     CDS             complement(2860..4152)
                     /gene="eno"
                     /locus_tag="VKU03_08625"
                     /EC_number="4.2.1.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004634 - phosphopyruvate hydratase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopyruvate hydratase"
                     /protein_id="HLJ71367.1"
                     /translation="MSEPVIQALRGRRIWDSRGRPTIEAEVLLSDGAAGRGVAPAGAS
                     RGRNEAVDKRDGGARLGGYDVQNALSGLRAEIAPALLGRDPFDQAGIDARLAALDGTP
                     NKSRLGGNALTAVSLAVLHAAAASRGQPLWRHLAGDGEALIPLPEIQIFGGGAHAGRR
                     VDIQDFMVVAPRAASFAEALEITAEVYLAAGRIMAERGLSEGVADEGGFWPAFASNEE
                     ALDTLMRAIEKAGLAPGEDVAISLDVAASEFGRGGRYKLALESRELDRDGFAEYLLRW
                     LDRYPIVSIEDPFAEDDAEGFMRFTAAVGGRVQVIGDDFLVTNAGRVRAAAAEKTVNA
                     VLVKVNQAGTVSEAKAACEEARRAGFGAIVSARSGETEDTTIVHLAVGWAAGQLKVGS
                     FARSERMAKWNEALRIEEALGPKARFAGMTALPLHAGR"
     gene            complement(4149..5072)
                     /locus_tag="VKU03_08630"
     CDS             complement(4149..5072)
                     /locus_tag="VKU03_08630"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside phosphotransferase family
                     protein"
                     /protein_id="HLJ71368.1"
                     /translation="MEPLTGGVSSDISLIEADDRRFCVKRALSRLKVAALWEAPTRRN
                     AAEAAYFRVVAGWLPHAAPAVLGEDSEAGLFAMDYFPPEDHPLWKAELLAGRVDTDFA
                     AAVGRDLAAIHARSAADPTIPAAFAHDETFEAIRIEPYLRATGRAHPSLAERFEALAA
                     RTLATKRALVHGDVSPKNILIGASGPVFLDAECAWFGDPAFDLAFCLNHLLLKGARSG
                     AVREPYLAAFRALAQIYLEGVAWEAADDLEARAASLLPALFLARIDGKSPVEYLTREA
                     ERDAVRRCAAPLIAAPPRRLRDVAEAWSRTR"
     gene            5275..5802
                     /locus_tag="VKU03_08635"
     CDS             5275..5802
                     /locus_tag="VKU03_08635"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607852.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046316 - gluconokinase activity [Evidence
                     IEA];
                     GO_process: GO:0046177 - D-gluconate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase"
                     /protein_id="HLJ71369.1"
                     /translation="MQKTNVQGSLVVMGVSGSGKTTVGLRLAKICGADFADGDDFHSP
                     EARAKMASGTPLTDEDRWPWLDRIGAALADRAAHPNGLIVACSALRRVYRDRLRASAG
                     PALRFLYLEGDKALMRSRVAARKGHYMPASLVDSQFATLEDPRGEADVVTMAADADLD
                     KELPEVLGRLSAIHD"
BASE COUNT         1034 a         2029 c         2008 g          928 t
ORIGIN      
        1 ggacgacgac aagctgcgat gggtctatct cgacatcggc aagttcaacg gcctcgccga
       61 gacgatggac gaaatgatcc gttatccgat caagacgccg aatgacgagg acgccatggc
      121 gccttgcgtg atcgccggcc cgacctgcga ctcggtcgac gtgctctacg agaaggagcc
      181 ctatctcctt ccggtgagcc tggagatcgg cgccaaggtg ctgatcgaag gaacgggcgc
      241 ctatacgacg acctattccg ccgtcggctt caacggcttc cagccgctga aatcctacgt
      301 gatctaagcg tcgcgttcag cgcgcgttcg atctgaaatc cggcgaccgc tccggatttc
      361 tcgcgtaacc atcccgaacg gcccgcgcgc ttcaaggaag cgcgcgtggc gcgagggtcg
      421 tcaacgctcc agcctttgga ggcctcgatg actctaatgt tcgatccaac tcgcctgtcc
      481 gcctgcgccc tgccgcccga cgcatcggct cgccttcggc ccatcgtcat agagaacgag
      541 cggccggccg atgggccggc gcgcgaagcc ttgctcgacg aagctttcgg cgaggagcgc
      601 ttcgccaaaa cctgccagcg cctgcgcgac ggccgcgcga cggccgaagg tctggctctt
      661 gtcgcgcgcg acggcgaggc gctggtcggc accttgtgct gctggaatgt gactgccggc
      721 gatcgcccgg cattgctgct cggcccggtg gccgtcgcca gatcccatcg ctcgatcgga
      781 atcggctcgg ccctgatgcg cgaagcgctg ttgcgcgcgg cggcgtgcgg ccacaaggcc
      841 gtgctgctcg tcggcgacgc gccctactat tgccgcttcg gcttcgcggc ctcgctgaca
      901 cgcggcctcg acctgcccgg cccggtcgaa cgcgcgcgct tcctcggctt cgagatcacg
      961 ccaggcgcgc tcaaaggctc gaaaggaatg gttaccgccg ccgacgttcc ggcctgcaaa
     1021 cgcgcgtcct cgttgaggcg cgcggcttag aggaaggggg accggcggaa ggccggtgga
     1081 agggctgcgg agttagacga ttatcctgaa ccgccggagc ccctccacgc tgacgcgcgg
     1141 ttcccacccg cgtcgcgggg gaggaaaggc gtcacaggtt cttcgccaga atcgcgacga
     1201 tcgacaagca gaagccgagc agccgcggca ggaaggcgag gctgccgccg gcgtgggcga
     1261 gcttcgatca tcgctcgccg gttgcgaaga gccggcggcg tccacacatg aggcgatgcg
     1321 gcggcggcga ggcggcgctc gcgtgaaacg gggacttgca agataacgac gaacagcgag
     1381 gcggccaagc cgctcaaggg ccgcgtcgcg ctggtgaccg gcgcgtcttc cggcatcggc
     1441 gaggcgacgg cgcgagccct ggccggagcg ggcgcgaaaa ccgtgattgc ggcgcggcgc
     1501 gccgagcggc tcgacgcgct ggcgaaagca atcgaaaaga atggcggcga agcgctggcg
     1561 ctcgccgccg acgcttcgct cgaaagcgag gccaggcgca tgctcggcga cgtcgagaaa
     1621 agctacggcc ggctcgacat tctggtcaac aatgccgggg tgatgctgct atcgccggtc
     1681 gaggaagcgg agatcggcga tttccgccgc atgatcgaga tcaacctgtt cggcctgatg
     1741 accctgtcgc ggctcgcctt gccgatcatg aagcgcggcg gcgccggcca tttcgtcaac
     1801 atctcttcgg tggccggccg catcgccaat cccggcgccg gcgcctatgc ggcgacgaaa
     1861 ttcggcgtcg tcgccttctc cgagtcgctg cgccgcgaag tctacaagga caagatcagg
     1921 gtcacggtga tcgaacccgg catggtcgcg accgagcttc ccgaccacat caccaatccc
     1981 gccatgcggg ccggcctcga gcagcgcaag gcggcgatgg agccgctcga agcggaggac
     2041 atcgccgcgg cggttctcta tgcggtcacg cagccgccgc gcgtcaacgt caacgaaatc
     2101 ctgatccggc cgacgctgca ggagcgatga gagcgcaatc cgtcatggcg agcaaagcga
     2161 ggcaatccac tgatagggta cagtcttatg gatggattgc ttcgtcgctt cgctcctcgc
     2221 aatgacggac gtaccgcagt cagcgccgct ttcgcgccga tttcggcttc ggctgcgcgt
     2281 ggcgccaggc ggcctccagc gcgacgcgaa gctcggcctc gtcgagcgcc gaaagctcgg
     2341 cgcgcgtcca gccggccgcg ccccagcctc ccttcagcgg cgcaaaagcc tgcggcgcaa
     2401 tcaggcattt gaactcctgc tcgtcgggcg caaacctgat attggcgctc tggcggtccg
     2461 cggcgagcgt cgcgaaaatg cgcgcggcgc gaaaagcgag acgatcgaaa tgcggcgcct
     2521 cgctgacgcc ggccagcgac agcgctatgc gccgcaaatc ttcgccggtc gccattttca
     2581 gacttccacc gcccaatcct actcgggaac ttcaacatca tatccgcgcg aacgcgccgc
     2641 ctccgcaaga cggagatcga atgtcgccaa gcaatgccca gcctcgctgc tcaaagcgag
     2701 atggagcgga tcggctgccg acagtttcaa agcggcgtcg cgcacaagca tggcggcccg
     2761 ggcgcccgcc gcgcatttgc tcacgaagat gtcgaactcg cgcgcgacca tcttaacacg
     2821 aattctgttt tttagaaaga ccgaattggg ttggcctgct catcgccccg cgtgcagcgg
     2881 cagcgccgtc atgccggcga agcgcgcttt cggccccagc gcctcctcga tgcgcaacgc
     2941 ctcgttccat ttcgccatcc gctcggatcg ggcgaacgag ccgaccttca attggccggc
     3001 ggcccagccg accgcgagat ggacgatcgt cgtatcctcg gtctctcccg agcgcgccga
     3061 gacgatcgcg ccgaagccgg cgcggcgggc ttcctcgcaa gccgccttcg cctcgctgac
     3121 cgtgccggcc tggttgactt tcacgagcac cgcgttgacc gttttctccg ccgcggcggc
     3181 gcgcacgcgg ccggcattgg tgacgagaaa atcgtcgccg atcacctgga cgcggccgcc
     3241 gaccgccgcg gtgaaacgca tgaaaccttc cgcgtcatcc tcggcgaaag ggtcctcgat
     3301 cgacacgatc gggtagcgat cgagccaacg caaaagatat tcggcgaagc cgtcgcgatc
     3361 gagttcgcgg ctttcgagcg cgagcttgta gcgcccgccg cggccgaatt ccgaggcggc
     3421 gacgtcgagc gagatcgcga cgtcctcgcc aggcgcgagg ccggctttct cgatcgcccg
     3481 catcagcgta tcgagcgctt cttcattgct ggcgaaggcc ggccagaagc cgccctcgtc
     3541 ggcgacgccc tcgctgaggc cgcgttcggc catgatccgg ccggcggcga ggtagacttc
     3601 ggccgtgatc tccagcgctt cggcaaaact cgcggcgcgc ggcgcgacca ccatgaagtc
     3661 ctggatgtcg acgcgccgcc ccgcatgggc gccgccgccg aaaatctgga tttcgggcag
     3721 cgggatcagc gcctcgccgt cgccggcgag atggcgccag agcggctgcc cgcggctcgc
     3781 cgccgccgca tggagaaccg cgagcgagac ggcggtcagc gcattgccgc cgagccgcga
     3841 tttgttcggc gttccgtcaa gcgccgccaa gcgggcgtcg attcccgctt gatcgaacgg
     3901 atcgcggccg agcaaagccg gcgcgatttc ggcgcgcaat cccgaaagcg cattctgcac
     3961 gtcatagccg ccgagacggg cgccgccgtc gcgcttgtcc accgcttcgt tgcggccgcg
     4021 cgacgcgccg gccggcgcga cgccgcgccc cgccgcgccg tcgctcaaca ggacttccgc
     4081 ttcgatggtc ggccttccgc gcgaatccca gatgcgccgg ccgcgcaagg cttgaatgac
     4141 aggctcgctc aacgcgttcg gctccaggct tcggcgacat cgcgcaagcg gcgcggcggc
     4201 gcagcgatca aaggcgccgc gcagcggcgc accgcgtcgc gctcggcttc gcgcgtcaga
     4261 tactcgaccg gcgacttgcc gtcgatccgg gcgagaaaca atgccggcag cagcgacgcc
     4321 gcgcgcgctt cgagatcgtc ggcggcctcc caagcgacgc cttcgagata gatctgggca
     4381 agcgcgcgaa aggcggcgag ataaggctcg cggaccgcgc cgctgcgcgc gcctttcaac
     4441 agaagatgat tcaggcagaa ggcgaggtcg aaagccggat cgccgaacca ggcgcattcg
     4501 gcgtcgagga agaccggccc gctcgcgccg atcaggatgt ttttcgggct gacgtcgcca
     4561 tgaaccagcg ccctcttggt cgcgagcgtg cgcgcggcga gcgcctcgaa gcgttcggcg
     4621 agcgaaggat gcgcgcggcc ggtcgcgcgc aaatagggct cgatgcggat cgcctcgaac
     4681 gtctcgtcat gggcgaaagc cgccggaatg gtcggatcgg cggcgctcct cgcatggatc
     4741 gcggcgaggt cgcggccgac ggcggcggcg aaatccgtat cgacgcggcc ggcaagaagc
     4801 tcggctttcc agagcggatg gtcctcaggc ggaaaatagt ccatcgcgaa gaggcccgcc
     4861 tcggaatctt cgccgagcac cgccggcgcg gcgtgcggca gccacccggc gacgacgcgg
     4921 aaataggccg cctccgccgc gttgcggcgc gtcggcgcct cccacaaagc cgcgactttg
     4981 agccggctca gggcgcgctt gacgcaaaag cggcggtcgt ccgcttcgat cagcgaaatg
     5041 tccgaagaga cgccgccggt gagcggctcc acgcgaacgg cctcgttcgg tccaacgagg
     5101 cccatgcggc gcaaggcggc caggagatcg gccggcggct cggaggatat aagcgaaatg
     5161 agccgcactc ccaggatgtc gcgagaatcg cgcgcgacga taagatattc gtggcggaga
     5221 gcgccagttt tatgcttgct tctccttccc gcgtcccgcc aagagcgcat ccgcatgcag
     5281 aaaacgaatg tgcaaggctc cctcgtcgtg atgggcgttt ccggcagcgg caagaccacg
     5341 gtcggcttgc ggctcgccaa aatctgcggc gcggattttg cggacggcga cgattttcat
     5401 tcgcccgagg cgcgggccaa gatggcgagc gggacgccgc tgaccgacga ggaccgctgg
     5461 ccctggctcg accggatcgg cgccgccttg gccgatcgcg cggcgcatcc gaacggcctg
     5521 attgtcgcct gctcggcttt gcgccgcgtc taccgcgacc gcttgcgcgc cagcgccggg
     5581 ccggctttgc gctttctcta tctcgaaggc gacaaggcgc tgatgcgaag ccgcgtcgcc
     5641 gcccgcaagg gccattacat gccggcctct ctggtcgaca gccagttcgc cacgctcgaa
     5701 gacccgcgcg gcgaggccga tgtcgtgaca atggcggcgg acgccgatct cgacaaggaa
     5761 ttgccggagg tgttggggcg gctgagcgcg atccacgatt aatcgagggt gaagcgaccg
     5821 gagtcggatc tcggcgccag gggccctctc cccgcttcgc gcagggctct ccggggaaag
     5881 taagaggcga gtcgcatcgt cgtcgacgaa gtctcgtgaa ataacgcgat ttcgagattt
     5941 cctcgttgag tttccggcgc cgagactcag aaaataaaca tgcggacctc ggggctgct
//
LOCUS       DATGJW010000280         2390 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_157606, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000280 DATGJW010000000
VERSION     DATGJW010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2390)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2390)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2390
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157606"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1944)
                     /locus_tag="VKU03_08640"
     CDS             complement(<1..1944)
                     /locus_tag="VKU03_08640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006465752.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HLJ71370.1"
                     /translation="MFESVLIANRGEIACRVIRTAKALGLRTIAIYSQADCGALFTRL
                     ADEAYEIGPAPARESYLNGDKIVALARKVGAASIHPGYGFLSENAEFAESCAAAGIVF
                     IGPPPSAIRAMGLKSAAKALMQKAGVPVTPGYHGDNQAPKFLKEKAYEIGYPVLVKAI
                     AGGGGKGMRRVDAHVDFEAALESAAREAQNAFGDARVLVEKYVSRPRHIEMQIFGDQQ
                     GNVVHLFERDCSLQRRHQKVIEESPAPGLSEETRAAMAAAAVAAGKAVGYVGAGTIEF
                     IADSSQGLQPNGFYFMEMNTRLQVEHPVTEAITGLDLVEWQFRVAAGERLPLSQEEIR
                     RSGAAVEARLYAEDPEHGFLPSSGKIFALRLPAGEALRVDAGVEEGGEVTPFYDPMIA
                     KIIAHGATRKEALDRLSEALGETLVAGPRVNARFLKALCDSEEFRAGAFDTGFIDANL
                     EKLGAAPHETDVTAALKGVAHLLSRRAAAYAEASPRDPWSVADGFELTGRRAVGVEAV
                     ADGRPLHLLETFQRGETHLTVKGEVASRRDERPATIIETERGVFVLREGRQTEVRLVD
                     PLDAEAEALAEGSGIIEAPMHGRLLALFVKEGDLVEKGARLAIVEAMKMEHALNAPRA
                     GRVAEIGYAAGAQVQQGARLMAIE"
BASE COUNT          419 a          793 c          727 g          451 t
ORIGIN      
        1 ctcgatcgcc atcaaccttg cgccttgttg tacctgcgcc ccagccgcgt aaccgatttc
       61 ggcgacgcgc ccggcgcgcg gcgcattcag cgcatgctcc attttcatcg cttcgacgat
      121 ggcgagccgc gcgccttttt cgacaagatc gccctccttg acaaaaagcg ccagcagccg
      181 tccgtgcatc ggcgcctcga taattcctga cccctccgcc aaagcttccg cttcggcgtc
      241 gagcggatcg acgagcctca cttcagtctg gcggccttct cggagtacga agacgcctct
      301 ttcggtttcg attatcgtcg ccggcctctc gtctcgacgc gaggcgacct ctcctttgac
      361 cgtcaagtgc gtctcgccgc gctggaaagt ttcgaggaga tgaaggggcc gcccgtccgc
      421 cacggcctcg acgccgacgg cgcggcgccc ggtcagttcg aagccgtcgg cgacgctcca
      481 aggatcgcgg ggcgacgcct cggcgtaagc tgcagcgcgg cgcgatagga gatgcgcgac
      541 gcctttcaac gctgcagtca catccgtttc atgcggcgcc gcgccgagct tttcgagatt
      601 ggcgtcgatg aaacccgtgt cgaaagcgcc ggcgcgaaat tcttctgaat cgcagagcgc
      661 tttgagaaag cgcgcattga cgcgcgggcc ggcgacgagc gtctcgccaa gcgcttccga
      721 caggcggtcg agcgcctctt tccgcgtcgc gccatgcgcg atgattttcg cgatcatcgg
      781 gtcgtagaaa ggcgtcactt cgccgccttc ttcaacgccg gcgtcgacac gcaaggcttc
      841 gccggccggc aatcgcagcg cgaagatttt accggaggac ggcaagaaac cgtgctcagg
      901 gtcctcggcg tagaggcggg cctccaccgc cgcgccgctt ctgcggattt cttcttggct
      961 tagcggcagt ctttcgcccg ccgcgacgcg gaactgccat tcgaccagat cgaggccggt
     1021 gatcgcctcg gtcactggat gctccacctg gagacgcgtg ttcatctcca tgaagtagaa
     1081 gccgttgggc tggagacctt gtgaggaatc cgcgatgaat tcgatcgtgc cggcgccgac
     1141 atagccaacc gctttgcccg cagcgacagc ggcggcggcc atggccgcgc gcgtctcttc
     1201 gctcaagccc ggcgccggac tctcctcgat gaccttctga tggcggcgtt gcagcgagca
     1261 gtcgcgctcg aagagatgga cgacgttgcc ctgctgatcg ccgaaaatct gcatctcgat
     1321 atgacgcggc cgcgacacgt atttttcgac gagaacccgc gcgtcgccga aagcgttctg
     1381 cgcctcgcgc gccgcgcttt cgagcgccgc ttcgaaatcg acatgagcat cgacacggcg
     1441 catgcccttg ccgccgccgc cggcgatcgc cttgaccagg accggatagc caatttcgta
     1501 cgctttctcc ttgagaaatt tcggcgcctg attgtcgccg tgatagccgg gcgtcaccgg
     1561 cacgccggcc ttctgcatca acgccttggc cgcgctcttg agccccatgg cgcggatcgc
     1621 agagggcggc ggtccgataa agacgatccc ggcggcggcg cagctttccg cgaattcggc
     1681 gttctcggag agaaatccat agccgggatg gatcgacgcc gcgccgactt tgcgggccag
     1741 cgccacgatc ttgtcgccgt tcagataact ctcgcgcgcc ggcgctgggc cgatctcata
     1801 ggcttcgtca gcgagccgcg tgaagagcgc gccacaatca gcctgcgaat agattgcgat
     1861 tgttcgaagg ccgagcgctt tggccgtccg gatgacacgg caggcgattt cgccgcgatt
     1921 ggcgataaga acggattcga acaatcggcg tctccttacc gcgataccct aatgcaggcg
     1981 ttcgggattg gccaacacgc ccccgatccc ggtcgtcgcc cggcggtgat agcaggaccg
     2041 aaaagcgtgc gccgagtcgc aatgctaagg cctcgcacat ttcgaccggc gtcaaaacgt
     2101 ccgcccttct ctggcgctac aagctgccca ttggcgaacg cggcggaggc catgagtaca
     2161 atcaagacaa acatgacgct ggaattggct cttctcgttg cgctctctgg ggcgcgtcct
     2221 acataggggc tgctgcgtcg tcggagcgaa ttgcttcgtc gcttcgctcc tcgcaatgac
     2281 gactgactaa ccgagcgcct cgatataatc cgtgacctca gccgagaaga tatagcgctc
     2341 ggcgctcacg agcggcgcgc catgggcgat cagaacgcgc gcgcaaccga
//
LOCUS       DATGJW010000281         5646 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_985605, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000281 DATGJW010000000
VERSION     DATGJW010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5646)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5646)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5646
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_985605"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1213
                     /locus_tag="VKU03_08645"
     CDS             <1..1213
                     /locus_tag="VKU03_08645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016836 - hydro-lyase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FumA C-terminus/TtdB family hydratase beta
                     subunit"
                     /protein_id="HLJ71371.1"
                     /translation="YEKKNLRYSQLAPLSMFEEKNTGDNLPAQIEILAEGEDTYKFLF
                     IAKGGGSANKSFLYQGLPSMLTHDRMLAFLKEKILTLGTAACPPYHLAVVIGGTSAEQ
                     TMKTVKLASTRYLDTLPISGGPDAHGFRDLAMEEEIFKLTQSLGVGAQFGGKYFCHDV
                     RVIRLPRHGASLPIGLGVSCSADRQALGKISRDGVFLEELERDPARFLPRIDEAALGG
                     EVVRVDLNAPMKDILATLAKHPIRTRLSLSGPMIVARDLAHAKIRERLERGEPMPDYF
                     KRHPVYYAGPAKTPAGYASGSFGPTTAGRMDSYVDQFQAAGGSMVMLAKGNRSKQVRD
                     ACAKHGGFYLGSIGGPAALLAQESIRKVEVLEYPELGMEAIWRIDVQDFPAFVVIDDK
                     GNDFFKELNLG"
     gene            1306..1569
                     /locus_tag="VKU03_08650"
     CDS             1306..1569
                     /locus_tag="VKU03_08650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71372.1"
                     /translation="MAIERGSPLEIAKRAASRRAKGRADADARPGAWRRETYCLPRSQ
                     AREKAQEWFQRYPKAAYMTEIEFWRELADGRIEFTIRRLPSAD"
     gene            2027..2692
                     /locus_tag="VKU03_08655"
     CDS             2027..2692
                     /locus_tag="VKU03_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein"
                     /protein_id="HLJ71373.1"
                     /translation="MKKILIGALLAGVATSAFAADLPTRKGPPPAPYTPPPIFTWTGF
                     YLGVNGAGAWGSMRHNDGAFNDPSGGMGGVTAGYNYQIGQFVAGIEGDFDWGDVSSRR
                     SLDAAGSFGKINMGEFATARARLGVAFDRALLYVTGGYAGGEVHSRLLDATTATFTSD
                     DSWRNGYAVGAGLEYAFTQNVSAKAEYLFSQIGGKTLATPTYAGRTGLDISLVRAGLN
                     YRF"
     gene            complement(2752..3360)
                     /locus_tag="VKU03_08660"
     CDS             complement(2752..3360)
                     /locus_tag="VKU03_08660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLJ71374.1"
                     /translation="MLVLRSSPPSPFGRKVRIGAAILGLADRIELANADTNDPDEPLR
                     RQNPLGKIPTLILENGAALYDSRVILDYLDHLAGGGRIIPREPEPRFAALTMQALGDG
                     LIDAAILQVYETRFRAPETHSAKWLDHQAGKVARALTALEAAPPPDDARHVGAIAVAC
                     ALGYLDLRFAGRWRANHPRLVRWLDAFAAGVPAFAATRVDPA"
     gene            3546..4520
                     /gene="trxB"
                     /locus_tag="VKU03_08665"
     CDS             3546..4520
                     /gene="trxB"
                     /locus_tag="VKU03_08665"
                     /EC_number="1.8.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018387053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004791 - thioredoxin-disulfide reductase
                     (NADP) activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin-disulfide reductase"
                     /protein_id="HLJ71375.1"
                     /translation="MSAPSAASHGRVVIIGSGPAGYTAAIYAARAMLSPILIAGVEAG
                     GQLMITTDVENYPGYAEPIQGPWLMEQMRLQAEHVGARIESDYVTRLDLGQRPFRVHC
                     DSGRVFTADSVILATGAKAKWLELPSEQAFKGYGVSACATCDGFFFRGKKVAVIGGGN
                     SAVEEALYLSNIAAHVTIVHRRDAFRAEKILQERLLQRPNVEVIWNHVVDEVVGSREP
                     VAVAGLRLRDAQTGAPRELAVDGVFVAIGHAPASELVRGQLTLRPNGYVQTAPGSTAT
                     SVEGVFAAGDVADDVYRQAVTAAGLGCMAALEAERWLAHLPQMQQAAE"
     gene            4581..5474
                     /locus_tag="VKU03_08670"
     CDS             4581..5474
                     /locus_tag="VKU03_08670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ71376.1"
                     /translation="MDWDKLRIFQAAADAGSFTHAGELMGLSQSAVSRQVSALEQDLG
                     TPLFHRHARGLILTEQGELLLNAVQDVVLKLEAARSRLIDSRDKPHGELRVTATLGFG
                     AIWLSPRLGEFLELYPEVKLQVLLDDDELDLGMREADIALRLREPSQPDLIRRRLFTV
                     HFHAYASAEYLKRNGQPRTVGDLDSHRILAFGPVGANHILTNLNSLLTLGRDPRTPRA
                     PSLTINNISAVTRAVEHGVGIAVLPDYCVTAEAGLVRLLPQADMPEMECFLVYPEEMK
                     NVARVQAFRDFLVAKAQRWKY"
BASE COUNT          976 a         1808 c         1834 g         1028 t
ORIGIN      
        1 gtacgaaaag aagaaccttc gctattcgca gctcgcgccg ctctcgatgt tcgaggagaa
       61 gaataccggc gacaatctgc cggcgcagat cgaaatcctt gccgagggcg aggatacgta
      121 caagtttctg ttcatcgcca aaggcggggg gtcggccaat aagagctttt tgtaccaggg
      181 cttgccgtcg atgctgacgc atgaccggat gctcgccttt ctcaaggaga aaatcctcac
      241 gctcggcacc gccgcttgcc ctccctatca tctcgccgtg gtgatcggcg gaaccagcgc
      301 cgagcagacg atgaaaaccg tcaagctcgc ctcgacccgc tatctcgaca cgctgccgat
      361 ttccggcggc ccggatgcgc atggcttccg cgatctggcg atggaggagg agattttcaa
      421 gctgacgcag tcgctcggcg tcggcgcgca attcggcggc aagtacttct gccatgatgt
      481 ccgggtgatc cggctgccgc gccatggagc gtccttgccg atcggcctcg gcgtttcgtg
      541 ctcggcggac cgccaggcgc tcggcaagat cagccgcgac ggcgtgtttc tcgaagagct
      601 ggagcgcgat cccgcgcggt ttctgccgcg catcgacgag gcggcgcttg gcggcgaggt
      661 ggtgcgcgtc gacctcaacg cgccgatgaa agacattctc gcgacgctcg ccaagcatcc
      721 catccgtacc cgtctctctc tctccggccc gatgatcgtc gcgcgcgatc tcgcgcacgc
      781 caaaatccgc gagcgcttgg agcggggcga gccgatgccc gattatttca agcgccaccc
      841 cgtctattac gccggcccgg ccaagacgcc ggccggctac gcgtcgggct ctttcggccc
      901 gacgacggcc ggccgcatgg attcctatgt cgatcagttc caggccgccg gcggctcgat
      961 ggtgatgctc gccaagggca atcgctccaa gcaggtgcgc gacgcctgcg ccaagcatgg
     1021 cggcttttat ctcggctcga tcggcgggcc ggccgcgctt ctggcgcagg agagcatccg
     1081 caaggtggag gttctcgaat atcccgaact cggcatggag gcgatctggc ggatcgacgt
     1141 ccaagacttc cccgccttcg tcgtcatcga cgacaagggc aacgatttct tcaaggagct
     1201 caatctcggc tgatccgctg cgacatcctt ttagaatcgc ccgccttttc cctctttcgc
     1261 cgtcgccgca tcccgcgcta agctcatcaa gcgcaaggag cggatatggc gatcgagcgc
     1321 gggtctcccc tcgaaattgc caagcgcgcg gcgagccgcc gcgccaaggg acgcgctgac
     1381 gccgatgcgc ggccgggagc gtggcggcgc gagacttatt gcctgccgcg cagccaggcc
     1441 cgcgaaaagg cgcaggaatg gttccagcgc tatcccaaag ccgcctacat gaccgagatc
     1501 gaattttggc gagaactcgc cgacggccgg attgagttta cgatccggcg gctgcccagc
     1561 gctgattgag gcaaatcggc agtcggcaat gggcagtcgg cagtcgggtt gcttaaaccg
     1621 actgccgact gcccattgcc gactgccgat acggcataga tatccggctc ggcgccatgc
     1681 ttaggcgcag ttacgcccag tcgtccgtcg gtggtttttc cgcgctgcgg caaggagaat
     1741 gagagaataa acgatttgtt caccctgttg aatttcgact ataaagctca cttttttgtg
     1801 aggtcgagta aaactcaaag gtttatttcg agaattaaca gcagatgaac cgcttaattc
     1861 aagatcaaaa tacttaccga taatcgccag attttggcca tattggccgc ctcgattgtg
     1921 acgccagtgc aacagacttt tcgacacaat cagcgttata gaacggtcca tctcgggggc
     1981 gggctgtcga gttcctcccg ctgggtccgg aaacgaggag ttttcaatga aaaagattct
     2041 aatcggggcg ctcctggctg gcgtcgccac ctcggccttt gcggcggatt tgccaactcg
     2101 gaagggtccg ccgccggccc cctatacgcc gccgccgatc ttcacctgga ccggcttcta
     2161 tctcggcgtg aacggcgcag gcgcgtgggg aagcatgagg cacaatgacg gcgctttcaa
     2221 cgatccaagc ggcggcatgg gcggcgtcac cgccggctac aactatcaga tcggccagtt
     2281 cgtcgccggc atcgaaggcg atttcgactg gggcgacgtc tccagccgcc gctcgctcga
     2341 tgcggccggg tcgttcggca agatcaacat gggcgagttc gcgaccgcgc gcgcgcgttt
     2401 gggcgtcgcg ttcgatcgcg ccctgctcta cgtcaccggc ggttacgccg gcggcgaggt
     2461 ccatagccgc ctgctcgacg cgaccacggc gacgttcacg tcggacgaca gttggcggaa
     2521 cggctatgcg gtcggcgccg gcctcgaata cgccttcacg cagaacgttt cggccaaggc
     2581 ggaatatctg ttctcgcaaa ttggcggcaa gaccctggcg acgccgacct atgccggccg
     2641 gacggggctc gacatttcgt tggtccgcgc gggcctcaac taccgcttct gatcagtcaa
     2701 tcgctccatc gatgggaccc ggcgggctcg cccgccgggt tttcctttgg ctcaggccgg
     2761 atcgacccgc gtcgccgcga aagccggaac gcctgcggcg aaagcgtcga gccagcgcac
     2821 cagtcgcgga tgattcgccc gccagcgccc ggcgaagcgc aaatcgagat aaccaagcgc
     2881 gcaggcgacc gcgatcgcgc cgacatggcg ggcgtcgtcg ggcggcggag cggcctccag
     2941 tgcggtcaag gcgcgagcga ccttgccggc ttggtggtcc agccatttgg cggaatgggt
     3001 ttccggggcg cggaagcggg tctcgtagac ttgcaggatc gcggcgtcga tcaagccgtc
     3061 gccaagggct tgcatcgtca gagcggcgaa acgcggctcg ggctctcgcg ggatgattcg
     3121 cccgccgccc gccaggtggt cgagatagtc caggatcacc cggctgtcgt agagcgcggc
     3181 gccgttctcc aggatcaggg tggggatttt gccgagcggg ttttgccgcc gcaaaggctc
     3241 gtccgggtcg ttggtgtcgg cgttagccag ttcgatgcgg tccgccaagc cgagaatggc
     3301 cgcgccaatc ctgactttgc gcccgaaggg cgatggcggc gatgagcgca ggaccagcat
     3361 ggcgttcccc cggaaattat gacggttcct gaggtctggc cgattgtggc ccgccgatca
     3421 agccggggcc cgtcgtcgcg gctatgctct ggttgccgaa gagggcgggg cgcccatata
     3481 tagctctgca aaatccgagg ggcttggcgc caagcgctca aggcctgctt ttttggcgag
     3541 gcgtcatgtc tgctccgagc gccgcgtctc atggacgcgt ggtcattatc ggctcagggc
     3601 cggccggcta tacggcggcg atctatgcgg cgcgggcgat gctgtcgccg atcctgatcg
     3661 ccggcgtcga agcgggcggc cagttgatga tcacgaccga cgtcgagaat tatccgggct
     3721 acgccgagcc gatccaaggg ccgtggctga tggagcagat gcgcctccag gccgagcatg
     3781 tcggcgcgcg gatcgaatcc gactatgtga cccgcctcga tcttggtcag cggccgttcc
     3841 gcgtccattg cgacagcggc cgcgtcttca ccgccgattc ggtgatcctc gcgacgggcg
     3901 ccaaagcgaa atggctggaa ttgccgagcg agcaggcgtt caagggctac ggcgtctcgg
     3961 cttgcgcgac ctgcgacgga tttttcttcc gcggcaagaa ggtggcggtg atcggcggcg
     4021 gcaattccgc ggtcgaagag gcgctgtacc tcagcaacat cgccgcgcat gtcacgatcg
     4081 tgcatcggcg cgatgcgttc cgcgccgaaa aaattcttca agagcggctg ctgcagcggc
     4141 cgaacgtcga ggtgatttgg aatcacgtcg tcgacgaagt cgtcggttcg cgcgagccgg
     4201 tcgccgtcgc cggcctcagg ttgcgcgacg cgcaaaccgg cgcgcctcgc gagcttgcgg
     4261 tcgacggcgt cttcgtcgcg atcggccatg cgccggcgag cgaattggtg cgcggacagc
     4321 tcacgctcag gccgaacggc tatgtgcaaa ccgctccggg ctcgacggcg acaagcgtcg
     4381 aaggcgtctt cgccgccggc gacgtcgccg acgacgttta tcggcaggcg gtgaccgccg
     4441 ccggccttgg ctgcatggcg gccctggagg ccgaacgctg gctcgcccat ctgccgcaaa
     4501 tgcaacaagc cgcggaatga ggcgggcgct ttgaattttg gcgcgaggcg cggcgcgcgc
     4561 ggctcgaccg gggggaaatc atggattggg acaagctgcg catctttcag gccgcggctg
     4621 acgcgggctc cttcacgcat gccggcgagc tgatgggctt aagccaatcg gcggtcagcc
     4681 ggcaagtcag cgccctcgag caggatctcg gcacgccgct ctttcatcgc cacgcccgcg
     4741 gcctcatcct gacggagcag ggcgaattgc tgctcaacgc cgtgcaggac gtggtcctga
     4801 agctcgaagc ggcgcgcagc cgcctgatcg acagccgcga caagccgcat ggcgaattgc
     4861 gcgtcaccgc gaccctcggc ttcggcgcaa tctggctgtc gccgcgcctc ggcgagttcc
     4921 tcgaactcta tcccgaggtc aagctgcagg tgctgctcga cgacgacgag ctcgatctcg
     4981 gcatgcgcga agccgatatc gcgctgcgcc tgcgcgagcc gtcgcagccc gacctcatcc
     5041 gccgccgcct gttcacggtg catttccatg cctacgcgtc ggccgagtac ctcaagcgca
     5101 acggccagcc gcgcacggtc ggcgatctcg attcgcaccg catcctcgcc ttcggcccgg
     5161 tcggcgccaa tcacatcctg accaatttga attcgctgct gacgctcggc cgcgatccgc
     5221 gcacgccacg cgcgccgtcg ctgacgatca acaatatcag cgcggtgacg agggctgtcg
     5281 agcacggcgt cggcatcgcc gtgctgccgg attattgcgt gaccgcggag gccggtctcg
     5341 tacgtcttct gccgcaggcg gacatgccgg aaatggagtg tttcctggtc tatccggaag
     5401 agatgaagaa cgtcgcgcgc gtgcaggcgt tcagggattt cctcgtcgcc aaggcgcaaa
     5461 ggtggaagta ctagaacccc ttcgccccgc cttgcgcagg gctgtccgcg gaaagtctga
     5521 ggttatggcg cgtcttggac gtaatctttc agcgccgcgg cgccacgccg gccgatcgcc
     5581 gtcaacccgc catagcgccg gcaagctacg tgcaagatca tatccgcttg tgcggcgcta
     5641 tggcgg
//
LOCUS       DATGJW010000282         8141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_883529, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000282 DATGJW010000000
VERSION     DATGJW010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8141
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_883529"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..398)
                     /locus_tag="VKU03_08675"
     CDS             complement(<1..398)
                     /locus_tag="VKU03_08675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HLJ71377.1"
                     /translation="MSTWSQTFTYLDGEWFDGNRPIIGPRSHAFWLGSSVFDGARAFE
                     GVTPDLDQHCARLNRSAKAMWLKPIMSDEAITELAREGVKKFAKDAALYIRPMYWAER
                     SGATSVPPDPESTRFCLTIYEAPLPKPTGIA"
     gene            complement(531..2300)
                     /locus_tag="VKU03_08680"
     CDS             complement(531..2300)
                     /locus_tag="VKU03_08680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238871.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ71378.1"
                     /translation="MAEPQTNRRLAAILAADVVGYSRLMAADESGTLAALKHFRESLF
                     NPAVAAHNGRVVKLIGDGTLVEFISVVDAVECAIAVQNAVAARVLPPGQPGIQLRIGV
                     NLGDVIIDGDDIYGDGVNVAARLEPLAEPGGVCVSSIVHESVGNRIDGSFRDFGQVQV
                     KNIDRPIAIWKWRPGEAGAAPAKKPEREVKGAAPSIAVLPFDNMSGDPGQDYFSDGVA
                     EDIITDLAKIAGLIVIARNSSFAYKGKKFDVRDVARELNVRFVLEGSIRHAGNRVRVT
                     AQLIEATSGAHLWAERYDRDLTDIFAVQDDLTQRIVSALKVTLSPAEKALIADSGGSA
                     NVEAHDRFMRARELLLGPTQNRETFEQAVATLSEAVALDPKYAQAYAGLAWAYVLDYT
                     NQWSEDSARALEHARRYADQAVETDPREPLAYFASAMVAGRQRNLERAVADANAALAL
                     SPNFAPARGLMASFDLFAGRPLEAIAQMERNMRLDPVVSQQGLQFLGLAYLTAGKYET
                     AALVFRQRLVLAPGSDSARSCLASALGHMGEIDEARRVWDELKAINPNYSFAGHMGRL
                     PFQREEDVRRIAEGLTKAGLPTD"
     gene            complement(2401..2805)
                     /locus_tag="VKU03_08685"
     CDS             complement(2401..2805)
                     /locus_tag="VKU03_08685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010915505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71379.1"
                     /translation="MSAVVIIALSLHALSAVFWAGSTFALARDPKLGAEALFRPQMGA
                     AIVAVLTGAYLWRLLHEGGFGPVEGVLAFGALCAIVAAGAQGALVGGASRRLRQGAWP
                     EAEAARRIALGERIAAILLAITLICMVSARYV"
     gene            complement(2983..3411)
                     /locus_tag="VKU03_08690"
     CDS             complement(2983..3411)
                     /locus_tag="VKU03_08690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018208324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLJ71380.1"
                     /translation="MTEKPGVGCGAAILRDGRILLVERRKIPEAAHWSLPGGKVDFLE
                     RVEAAVARETKEEVGVGLQLGPLLCVTEMIGIDGQHWVSPIYRATIAEGEPVNCEPDK
                     IASIGWFPLDAPPSPLAQGARDAIAALRSPLTEGHGRGVR"
     gene            complement(3422..4804)
                     /locus_tag="VKU03_08695"
     CDS             complement(3422..4804)
                     /locus_tag="VKU03_08695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264174.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLJ71381.1"
                     /translation="MATIDDVLAKIDANQTGAIERLFSLLSIPSVSTDPARTDDCLRA
                     ADWLVDELKGLGFEISRRETDGKPMVVGHAKGVRRDAPHVLFYGHYDVQPPEPLELWE
                     KPPFAPRLVDAPTGQRIVARGACDDKGQLMTFVEACRAFRETGGLPCDVSVLFEGEEE
                     IGSPSLPGFMAKNAKELKADVMLVCDTAMWDRTTPAITTMLRGLVYEEVILRGANRDL
                     HSGLFGGAAINPIRVLARALADLHDENGAVTLPGFYDGVEELPEEIATQWRELDFNEE
                     EYLGEVGLSVPAGEKGRSVLEMVWSRPTCEINGIVGGFTGEGSKTVLPAEARAKVSFR
                     LVGRQNPDKIAQSFRDFVSARLPADVKAEFVSHASSPAISVATKSEALRRARRALESE
                     WGKPALLIGSGGSIPIVGAFKRELNMDALLVGFSLDDDKAHSPNEKYERSSFHKGARS
                     WARILAAIAA"
     gene            complement(4896..5792)
                     /gene="argB"
                     /locus_tag="VKU03_08700"
     CDS             complement(4896..5792)
                     /gene="argB"
                     /locus_tag="VKU03_08700"
                     /EC_number="2.7.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003991 - acetylglutamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006592 - ornithine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetylglutamate kinase"
                     /protein_id="HLJ71382.1"
                     /translation="MNDTRKASPQEQSEILMQALPHMLRYDDATVVVKYGGHAMGDDA
                     AARDFAKDMVLLEQSGVNPVVVHGGGPQIGVMLKKLGIESRFSGGLRITDKATVEIVE
                     MVLAGAINKQIVGLINAEGGRAVGLCGKDGNMVIARKVTRAAAGGNIEVEDVVDLGYV
                     GEPARVDTTVLDQILGRELIPVLAPVAQGADSETYNINADTFAGAIAGALGAKRLLLL
                     TDVPGVLDKDKQLIKELKVDQIRGLIADGTITGGMIPKVETCIYALERGVEGVVILDG
                     KTPHAVLIELLTDQGAGTLITK"
     gene            complement(5884..7809)
                     /locus_tag="VKU03_08705"
     CDS             complement(5884..7809)
                     /locus_tag="VKU03_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="potassium transporter Kup"
                     /protein_id="HLJ71383.1"
                     /translation="MQDALRGDVAPATNGHDAVNGAHQSAGNFALLALGSVGVVFGDI
                     GTSPLYALGASLSHAKDAGLPPGEVIGVVSLILWALIVTVTAKYVLFLMHADNRGEGG
                     ILSLMALAQSAIGRRTALIFFLGVAGSALFSGDAIITPAISVLSALEGLKQVSPGLSP
                     FVLPATIAILIGLFVVQSRGTARVAAFFGPVMAVFFVCIAVLGATHIPQAWQILLALN
                     PMRGLLYLFHDGAVGFIVLGSVFLAVTGAEALYADMGHFGRTPIQSAWVFLVLPALAL
                     NYLGQGAHLIADPSAVANPYYLLAPSWLLMPLVILSTIATVIASQAVITGAYSLARQA
                     IQLGLLPRLEIVHTSDVQEGQIYIPRANRLLLIGVLALVLLFRTSDALANAYGIAVSG
                     TMVATTSLAFFVVWRLWRWPLWSALLIVCGFLAIDISFFLANLYKVLDGGWIPLLLGG
                     TMVLVMWTWARGSHILSLKTHRDSIPMTDLIKMLEKSKPVRVPGTAIFLTSDPSVAPS
                     SLMHNLKHNKVLHERVVVMCVKTETTPRMKAAARFEVQKLSPDFLGVTLHFGYMESPR
                     IPHALALMRKAGWKFDIMTTSFFVGRRTLKAATNSDMPRWQDRLFIALAKQATNAPDF
                     FHIPSDRVVELGAQMKV"
BASE COUNT         1490 a         2634 c         2676 g         1341 t
ORIGIN      
        1 gcgatgccgg tcggcttggg cagcggcgct tcgtagatcg tcaagcagaa acgggtcgat
       61 tccggatccg gcggcaccga ggtcgcgccg cttcgctcgg cccaatacat cgggcggata
      121 tagagcgccg cgtccttcgc aaatttcttc acgccttcgc gggcgagctc ggtgatcgcc
      181 tcgtcgctca tgatcggctt cagccacatc gccttggccg agcggttgag tctcgcacaa
      241 tgctggtcga gatcgggcgt cacgccctcg aaagcgcgag cgccgtcgaa aacgctggag
      301 ccgagccaga aagcgtggct gcgcggcccg atgatcggtc tattgccgtc gaaccattcg
      361 ccgtcgagat aggtgaacgt ctgcgaccag gttgacatgt cggccctcgc tcctcgcctt
      421 gctgaattgg gaatttcaaa taaatgtcgc tcccgcgcaa gccgaaatcc agactgagcc
      481 gacgttcagg cgctctggat tcccgctttc gcagaatggc gcgtgtaccc ttagtcggtc
      541 ggcaagccgg ctttggtcag gccttcggcg attcgccgaa cgtcctcttc gcgctgaaac
      601 ggcagccggc ccatatggcc ggcgaaggaa tagttcgggt tgatcgcctt gagctcgtcc
      661 caaacgcggc gcgcttcatc gatctcgccc atatggccaa gggcggaggc gaggcaggat
      721 cgggcggagt cggagccggg cgcaaggacg agtcgctgcc tgaaaacgag cgccgccgtt
      781 tcgtactttc cagcagtcag ataggcgagg ccgaggaact gcagcccctg ctgactgacg
      841 accgggtcga gccgcatatt gcgctccatc tgcgcaatcg cttcaagcgg ccggccggca
      901 aacagatcga aactcgccat gaggccgcgc gccggcgcga aattaggact taaagccaag
      961 gcggcgttgg cgtccgcaac cgcgcgttcg agattgcgct ggcgtcccgc caccatcgcg
     1021 ctggcgaaat aggcgagcgg ctcgcgcggg tccgtctcga ccgcctgatc cgcatagcgc
     1081 ctggcgtgtt cgagcgcgcg cgcggaatcc tcgctccatt ggttcgtata atcgagcaca
     1141 taggcccagg ccaggccggc ataggcctgg gcgtatttcg ggtcgagcgc gaccgcttcg
     1201 ctgagcgtgg cgaccgcctg ctcgaatgtc tcgcgatttt gggtcgggcc gagcaggagc
     1261 tcgcgggcgc gcatgaagcg atcatgcgcc tcgacattgg cggagccgcc ggaatcggcg
     1321 atgagcgctt tttcggcggg actcaacgtc actttcaggg cggagacgat gcgttgcgtg
     1381 agatcgtcct gcacggcgaa aatatcggtc aagtcgcgat cgtagcgctc ggcccaaaga
     1441 tgcgcgccgc tcgtcgcctc aatgagctgc gccgtgacgc gcacgcgatt gccggcatgg
     1501 cggatgcttc cctcgagcac gaagcggaca ttcagctcgc gggcgacgtc cctcacgtcg
     1561 aatttcttgc ccttataggc gaagctcgaa ttacgggcga tgacgatgag gcccgcgatc
     1621 ttggcgaggt cggtgatgat gtcctcggcg acgccgtcgc tgaaataatc ctggcctgga
     1681 tcgcccgaca tattgtcgaa aggcagcacg gcgatcgacg gcgcggcgcc tttgacctcg
     1741 cgctccggct tcttggccgg cgcggcgccg gcctcgcccg ggcgccattt ccagatcgcg
     1801 atcggccggt cgatattctt gacctggacc tggccgaaat ccctaaacga gccgtcgatg
     1861 cggttgccga cgctctcgtg gacgatcgac gagacacaca cgccgcccgg ctcggcgagc
     1921 ggctcgaggc gagccgcgac attaaccccg tcgccgtaaa tatcgtcgcc gtcgatgatc
     1981 acgtcgccga gattgacgcc gatgcgcaat tggatgcccg gctggccggg cggcaagacg
     2041 cgcgccgcga cggcgttctg cacggcgatg gcgcattcga cggcgtcgac gacgctgatg
     2101 aattcgacga gcgtcccgtc gccgatcagc ttgacgacgc ggccattatg ggcggcgacg
     2161 gccggattga acaggctttc gcggaaatgt ttcagcgcgg cgagcgtgcc cgattcgtcc
     2221 gcggccatca ggcgcgaata gccgaccacg tcggcggcga ggatcgccgc gagccttcga
     2281 ttggtctgag gttccgccat ggcaatatgg tcgtatcagc ggacgggccg cccgccaatg
     2341 cgtgaaacgt catgtagggc ggaaaccgcc ggcgtcaatg cggggcgcca gagccgcggc
     2401 tcagacgtag cgcgcggaaa ccatgcagat cagcgtgatg gcgaggagaa tcgcggcgat
     2461 gcgctcgccc aaggcgatcc gccgggccgc ttcggcctcc ggccatgcgc cttggcgcag
     2521 ccgccggctg gcgccgccga ccagcgcgcc ctgcgcgccc gccgcgacga tggcgcaaag
     2581 cgcgccgaaa gccaagacgc cttcgaccgg gccgaagccg ccttcatgca gcagacgcca
     2641 cagataagcg ccggtcagca cggcgacgat tgcggcgccc atttgcggcc ggaacagcgc
     2701 ctcggcgccg agcttcggat cgcgggcgag cgcgaaagtc gagccggccc agaagaccgc
     2761 ggagagggcg tgaagggaaa gcgcaatgat cacgacggcc gacatcgctt gactccgatc
     2821 gatgagcgca aagggtatac gacgccaatg cggaagagac cagggcgcat ccgcgccatt
     2881 gttgcggcag gcgctcctcc cctctcccga agggagcggc cgggcgcgag gggtgcggcg
     2941 caagggaaga atccgtacat ttaccgtacc gaagcaccgg tttcaccgta cccctcgccc
     3001 atgcccctct gtgagaggcg atcgcagcgc tgcgatcgcg tcccttgcgc cttgcgcgag
     3061 cggcgaaggc ggcgcgtcga gcgggaacca accgatcgag gcgatcttgt cgggctcgca
     3121 attgaccggt tcgccctcgg cgatcgttgc gcggtagatc ggcgagaccc aatgctggcc
     3181 gtcgattccg atcatctcgg tcacgcaaag cagcggcccg agctggagac cgacgccgac
     3241 ctcctcctta gtctcgcgcg caaccgccgc ttcgacgcgt tcgaggaagt cgaccttgcc
     3301 gcccggcagg ctccaatggg cggcctcggg aatcttgcgg cgctcgacca acaggatgcg
     3361 gccgtcgcga aggatcgccg cgccgcagcc gacgccaggt ttctctgtca atgtcgcccg
     3421 ctcacgccgc gatcgccgcc aagatgcgcg cccaggagcg cgcgccttta tgaaaggacg
     3481 agcgctcgta tttctcgttc ggcgaatgcg ccttgtcgtc gtcgagcgag aagccgacca
     3541 gcagcgcatc catgttcaat tcgcgcttga acgcgccgac gatcgggatc gagccgccgg
     3601 agccgatcag cagagccggc ttgccccatt cgctttcgag cgcgcggcgc gcccggcgca
     3661 aggcctcgct cttggtcgcg acggatatcg ccgggctcga cgcgtggctg acgaattcgg
     3721 ccttcacgtc ggccggcagc cgcgcgctga cgaaatcgcg gaagctctgc gcgatcttgt
     3781 ccggattctg ccgcccgacc aggcggaacg acactttggc gcgcgcctcg gccggcagca
     3841 cggtcttcga gccctcgccg gtgaagccgc cgacaatccc attgatctcg caggtcggcc
     3901 gcgaccagac catctcgagg acgctgcggc ccttttcgcc ggccggaacc gacaggccga
     3961 cctcgcccag atactcctcc tcgttaaagt cgagctcgcg ccattgcgtc gcgatctctt
     4021 ccggcagttc ctcgacgccg tcatagaagc cgggcagcgt caccgcgcca ttctcgtcat
     4081 gcaagtccgc gagcgcgcgc gccagcacgc ggatcgggtt gatcgccgcg ccgccgaaca
     4141 agcccgaatg caaatcgcga ttggcgccgc gcaggatcac ttcttcgtag acgaggccgc
     4201 gcagcatcgt ggtgatcgcc ggcgtcgtcc ggtcccacat cgccgtgtcg caaaccagca
     4261 tgacgtcggc cttgagctct ttggcgttct tggccatgaa gcccggcagc gagggcgagc
     4321 cgatctcctc ttcgccctcg aacagcacgc tgacgtcgca aggcaggccg ccggtctcgc
     4381 ggaaggcgcg gcaggcttcg acgaaggtca tcaattgacc cttgtcgtcg caggcgccgc
     4441 gcgcgacgat ccgctggccg gtcggcgcgt cgacgagacg cggcgcgaaa ggcggcttct
     4501 cccagagttc gagcggctcg ggcggctgca catcgtaatg gccgtagaac aggacatgcg
     4561 gcgcatcgcg gcgaaccccc ttggcgtggc cgaccaccat cggcttgccg tcggtctcgc
     4621 gccggctgat ctcgaagccg aggcccttca attcgtcgac cagccaatcg gcggcgcgca
     4681 gacaatcgtc tgtccgcgcc ggatcggtcg agaccgaggg aatggacaga agagaaaaca
     4741 gacgttcgat cgcccccgtc tgattggcgt cgattttcgc taaaacatcg tcgatcgtgg
     4801 ccatggcgct ctccgcggcg catcttagac aatcgccgcg cgggaaacca gacgaggaga
     4861 gcggcgcccg aaacggcggc gcccgccgga tgggcctatt tcgtgatcag cgtcccggcg
     4921 ccctggtcgg tcagcagctc gatcaacacc gcatgcggcg ttttgccgtc gaggatgacg
     4981 acgccctcga cgccgcgctc caaagcatag atgcaggtct cgactttcgg aatcatgccg
     5041 ccggtgatcg tcccgtcggc gatcagcccg cggatttgat cgaccttcag ctccttgatg
     5101 agctgcttgt ccttgtcgag cacgccggga acgtcggtga gcagcaaaag ccgcttggcc
     5161 ccgagcgccc cggcgatcgc cccggcgaag gtatcggcgt tgatgttgta ggtctcgctg
     5221 tcggcgccct gcgcgaccgg ggcgagaacc ggaatgagct cgcggccgag aatctgatcg
     5281 agcacggtcg tgtcgaccct ggccggctcg ccgacatagc cgagatcgac cacatcctcg
     5341 acctcgatat tgccgccggc cgccgcgcgc gtgactttgc gcgcgatcac catgttgccg
     5401 tccttgccgc aaaggccgac cgcccggccg ccctcggcgt tgatcaggcc gacgatctgc
     5461 ttgttgatcg cgccggccag caccatctcg acgatctcga ccgtcgcctt gtcggtgatg
     5521 cgcaagccgc ccgaaaagcg cgattcgatg ccgagcttct tcagcatcac gccgatctgc
     5581 ggcccgccgc catggaccac gaccggattg acgcccgatt gttcgagcag aaccatgtcc
     5641 ttggcgaaat cgcgcgccgc ggcgtcatcg cccatggcgt ggccgccata tttgacgacg
     5701 accgtggcgt cgtcatagcg cagcatgtgc ggcagcgcct gcatcaggat ttcggactgc
     5761 tcctgcgggc tggctttgcg cgtatcgttc atggggctcg cggctccgga atagcgggcg
     5821 agggtcttct agcggctcgg cgccgcaagc tcaaatgcga gcgatgcgct tgccgggaag
     5881 ccgttagact ttcatctgcg cgccgagctc gacgacgcga tcggacggga tatggaaaaa
     5941 gtccggcgcg ttggtcgcct gtttcgccag cgcgatgaac agcctgtcct gccagcgcgg
     6001 catgtcggaa ttggtcgcgg ccttcagcgt gcggcggccg acgaagaagg acgtcgtcat
     6061 gatgtcgaac ttccaccccg ctttgcgcat caaggcgagc gcgtgcggaa tgcgcggcga
     6121 ttccatatag ccgaaatgca aggtcacgcc gagaaagtcg ggcgacagct tctgaacttc
     6181 gaagcgcgcg gccgccttca tgcgcggcgt cgtctcggtc ttgacgcaca tgacgacgac
     6241 gcgctcgtgc aggaccttgt tgtgcttcaa attgtgcatc agcgacgagg gcgcgacgct
     6301 cgggtcgctg gtcaggaaaa tcgccgtgcc cggcacgcgc accggcttcg atttctccag
     6361 catcttgatc agatccgtca tcgggatcga atcgcgatgg gtcttcagcg agaggatgtg
     6421 gctgccgcgc gcccaggtcc acatgacgag aaccatcgta ccgccgagca ggagcggtat
     6481 ccagccgccg tcgaggacct tatagagatt ggcgaggaag aaggagatgt cgatcgccag
     6541 gaagccgcag acgataagca gcgcgctcca caatggccag cgccacaagc gccagacgac
     6601 gaagaaagcc agcgaggtcg tcgccaccat cgtgccggag accgcgatgc cataggcgtt
     6661 ggccagcgcg tcggaggtgc ggaacagaag aaccagcgcg agcacgccga tcagcaacag
     6721 gcggttggcg cgcggaatgt agatttgccc ttcttgaacg tcggaggtgt ggacgatttc
     6781 aagacggggc agaaggccga gctggatcgc ctggcgcgcc aaggagtacg cgccggtgat
     6841 cactgcctgg ctggcgatga cggtcgcgat cgtcgagaga atgaccagcg gcatcagcag
     6901 ccaggacggg gccagcagat aatagggatt ggccaccgcg ctcggatcgg cgatcagatg
     6961 cgcgccctgg ccgagatagt tgagggcgag cgccggcagg acgagaaaaa cccaggccga
     7021 ttggatcggc gtccggccga aatggcccat atcggcgtaa agcgcttcgg cgccggtcac
     7081 cgccaggaac acgctgccga gcacgatgaa gccgaccgcg ccgtcatgaa agagatagag
     7141 cagaccgcgc attggattga gcgccagcag aatctgccag gcttgcggaa tgtgagtcgc
     7201 gccgagcacc gcgatgcaga cgaagaacac cgccatgacg gggccgaaaa acgcggcgac
     7261 gcgcgccgtg ccgcggcttt gaacgacgaa gaggccgatg agaatggcga ttgtcgccgg
     7321 cagaacgaag ggcgaaagac cgggcgagac ttgcttgagg ccctcaagcg ccgagagaac
     7381 cgaaatggcc ggcgtgatga tcgcatcgcc cgagaacaag gccgagccgg cgacgccgag
     7441 aaagaagatc agcgcggtgc gccggccgat cgcgctttgc gcgagcgcca tcagcgagag
     7501 gatgccgccc tcgccgcggt tgtcggcatg catgagaaag aggacatatt tggcggtcac
     7561 cgtgacgatg agcgcccaca ggatcagcga gaccacgcca atcacctcgc ccggcggcag
     7621 gccggcgtcc ttggcgtggc tgagcgaggc gccgagcgca tagagcgggc tcgtgccgat
     7681 atcgccgaac acgacgccga ccgagcccaa ggccagcaat gcgaaattgc cggccgattg
     7741 atgggcgccg ttgacggcgt catggccatt ggtcgcgggc gcgacgtcgc cgcgcaacgc
     7801 atcctgcata aaggttctct ctaaggaggt gaaagtcctg ggatccgatg tcccgcctat
     7861 ggcggcgccc ctatacaacg ccgcctctcc cgctggaagc ctcgcccgtc acgaaggatt
     7921 gctcaaggaa aacgcatggc tcttgcgtcg gcgcgggctt cctgcaaagg agccggaaat
     7981 gccctccgct tctgatattt tgccgataga cgagatttta gagcagcttc gcgccgctct
     8041 tgcggcaaaa agccgcgtcg ttctcgtcgc tccgccgggg gccggcaaga cgacccggac
     8101 gccgctggcg cttctcgatc agcttttttt gcatggccgc a
//
LOCUS       DATGJW010000283         9369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_54017, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000283 DATGJW010000000
VERSION     DATGJW010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9369
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54017"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..474)
                     /gene="dksA"
                     /locus_tag="VKU03_08710"
     CDS             complement(55..474)
                     /gene="dksA"
                     /locus_tag="VKU03_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019899 - enzyme binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase-binding protein DksA"
                     /protein_id="HLJ71384.1"
                     /translation="MGVVGITESYKPSEDEPFMNERQKEYFRRKLSDWKEAILKESRE
                     TLAALQSESENHPDIADRASSETDRAIELRARDRQRKLIAKIDAALARIEEGTYGYCE
                     ETGDPIALRRLDARPIATLSVEAQERHERRERVYRDD"
     gene            683..1108
                     /locus_tag="VKU03_08715"
     CDS             683..1108
                     /locus_tag="VKU03_08715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HLJ71385.1"
                     /translation="MFKRIVVPIDLAEIELAQPAIDQAVELAEASGGALRLVNVQPLL
                     PATFMDYVPADFDAAQEERAAKALEELAAGVRLPKERISHVVRVGGAYHEILAEARAW
                     AADLIVVGSHRPVMSDYLLGSNAKTIVRHAHCSVLVVRP"
     gene            complement(1158..2333)
                     /locus_tag="VKU03_08720"
     CDS             complement(1158..2333)
                     /locus_tag="VKU03_08720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018273364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HLJ71386.1"
                     /translation="MFLSFFAALRQAHVPVTPREYLTLMQALDRDLAEQSVEAFYHLS
                     RALLVKDERNLDKFDVVFAATFKGALSLAAAIERVEIPEEWLRKLVERYLTPEERAEL
                     KALGFDKLMETLRQRLAEQKGRHQGGSKWIGTAGTSPFGAYGENPEGVRIGQDKGRHG
                     RAVKVWDRRDFKDLDGDEELGPRAMKIALRRLRRFAREGAPDELDLDRTISDSAKKGY
                     IDVQLRPERRNAIKVLLFLDVGGSMDWHVEAAENLFAAARSAFKRLQHFYFHNCLYES
                     VWTKNRRRHEERTPTLDILNTYGRDYRAIFVGDASMSPYEITMPGGSVEHVNPEPGQV
                     WLERVTRAYPHAAWINPTPEPQWGYSQSTAMIRRIFGDRMYALTLDGLEGAMRELTR"
     gene            2514..2723
                     /locus_tag="VKU03_08725"
     CDS             2514..2723
                     /locus_tag="VKU03_08725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3311 domain-containing protein"
                     /protein_id="HLJ71387.1"
                     /translation="MTSLKWLAALPFIAILGGTAFFNSVEPLVLGLPLILAWLVLWVL
                     LTAAIMALIFFNDPANSDRAESDGR"
     gene            2720..4189
                     /locus_tag="VKU03_08730"
     CDS             2720..4189
                     /locus_tag="VKU03_08730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319823.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:solute symporter family protein"
                     /protein_id="HLJ71388.1"
                     /translation="MNSALIIIFLTAALALVLGLVARSGKEMTLEQWTVGGRGFGGIL
                     VFLLQAGEIYTTFTFLGGSGWAYGIGAQTYYILAYGCLAYILSYWLLPPVWRYARQQR
                     IVSQSHFVARKYDSPLLGVLVAAVGVVALVPYLVLQFKGLGIIVSVASYGAISSTAAI
                     WIGAAIITAYVMVSGVRGSAWTSTVKDLMILAVVVFLGIYLPLHYYGGYGDMFRAIDA
                     AKPGFLALKPSGDGGLIWFQSTALLSALGFYMWPHAFGAVFTAKAEHIFRRNAIMLPL
                     YQLILLFVFFCGFAAILKAPGLTGPDIDLSLFKLSINTFDPWFVGVIGAAGVLTALVP
                     GSIILVAASTLCANDVLRGLRREMREETVAWTARGLVPIVALVAVFFTLQGGKTIVAL
                     LLMGYSFVTQFFPAIVCSLMRNNPLTKAGAFAGIAVGVATVVAVTLSHSTLATLAPFL
                     PKPIQEINVGFLALILNVIVAAVVSAGARAATIASAERA"
     gene            4419..5756
                     /locus_tag="VKU03_08735"
     CDS             4419..5756
                     /locus_tag="VKU03_08735"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HLJ71389.1"
                     /translation="MRLSRYFLPILRETPKEAEIVSHRLMLRAGMVRQEAAGIYAWLP
                     LGLRVLAKITEIVREEQNRSGAIELLMPTIQSADLWRESGRYEDYGKEMLRIKDRHER
                     DMLYGPTNEEMITEIFRAYIRSYKDLPLNLYHIQWKFRDEVRPRFGVMRSREFLMKDA
                     YSFDLDAEGARHSYNKMFVAYLRTFLRLGLTAIPMVADTGPIGGNLSHEFIILATTGE
                     SEIYCHKDYLSFPPPPETIDFDDSAAVQAVVDKWTSLYAATSEKHDAAAFAALPETAR
                     LSARGIEVGHIFYFGTKYSEPMKAVVNGPDGKEHAIHGGSYGIGPSRLAAALIEANHD
                     EAGIIWPDAVAPFRVGLANLKVGDSATDAACGRIHAALEAAGIDVLYDDRDERPGAKF
                     ATLDLIGLPHQILVGPRSLAEGKVELKERRSGARDLVSVEDAIRRFGGQTERG"
     gene            5983..7287
                     /locus_tag="VKU03_08740"
     CDS             5983..7287
                     /locus_tag="VKU03_08740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0042953 - lipoprotein transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein-releasing ABC transporter permease
                     subunit"
                     /protein_id="HLJ71390.1"
                     /translation="MTAAALDVPTETAAPSLAARPFARFEWMVAFRYLRARRKHGSIS
                     VIAGFSFLGIVLGVAALIVVMSVMNGFRHDLLDKIIGLNGHLFLQPAETSLTDYDAVT
                     ERVRKVQGVKLALPLVEGQALASSQFNSSGVLVRGVREADLKQLPGIAGHINLGTLNG
                     FDSAGGAAIGARLAEHLGLRIGDKITIIAPRGAQTPFGVTPRIKSYPVVAIFQIGMFQ
                     FDNTFVFLPLPEAQAFFNLDGQVNVIEAFVDDPDRMDEMRQRIEPALQRPMIMIDWRQ
                     RDQTFFEALNVERNVMFAILTLIVLVAALNIISGLIMLVQDKARDIAVLRTMGATRGA
                     VMRIFLITGATIGVVGTLVGVLLGLLVAQNVEAIRHGLNWLLNANLFPAEAYFLSHLP
                     SRVEPGDVIAVVVMTLALSLAATLYPSWRAARLDPVEALRYE"
     gene            7280..7972
                     /locus_tag="VKU03_08745"
     CDS             7280..7972
                     /locus_tag="VKU03_08745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71391.1"
                     /translation="MNEAATPALWLHGVEKRFGRGETLLEILRGVDLALWSGQSVALI
                     APSGAGKSTLLHVAGLLETPDAGEVFIGGAGTAGMSDDQRTALRRNEIGFVYQFHHLL
                     PEFSAAENLILPQMIRGLSRAEAGKRASELLAYLGLGARERHRPAELSGGEQQRVAIA
                     RAVANGPRLLLADEPTGNLDPKTADHVFGTLTALVKASGLAALIATHNLDLAARMDRR
                     VTLQDGRLVELA"
     gene            complement(8026..8454)
                     /locus_tag="VKU03_08750"
     CDS             complement(8026..8454)
                     /locus_tag="VKU03_08750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71392.1"
                     /translation="MAMSFFRSTALAASLAASLAAIAPAMAGPVATAGPLGVAASEKT
                     AAPVETVAYRHHYHYRHHYYNGAGAAVAGAALGIIGMAMANSFYNDYDDCYYGGCGYY
                     YGGPAYTYGGPVYGGWGGGWHGYHHFHHFGGWHGGHYHHH"
     gene            complement(8603..9004)
                     /gene="acpS"
                     /locus_tag="VKU03_08755"
     CDS             complement(8603..9004)
                     /gene="acpS"
                     /locus_tag="VKU03_08755"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007693090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HLJ71393.1"
                     /translation="MIIGIGSDLCDIRRIEETLERYGERFVQRCFTEIERRKSEGRKD
                     RAASYAKRFAAKEACAKALGTGLRRGVFWRDMGVVNLPSGQPTMHMTGGAAARLAAII
                     PAGCAAYIHVTITDEHPLAQAFVVIEARPSA"
BASE COUNT         1501 a         3141 c         2981 g         1746 t
ORIGIN      
        1 ggggcggcgc ggcgcgaggc gcaaaaaagc cggctcgcgc cggtttacat tttatcaatc
       61 atcgcgatag acgcgctctc tgcgttcatg gcgctcttgc gcctccaccg acaaagtcgc
      121 gatcggcctg gcgtcgaggc ggcggagggc gatgggatcg ccggtctcct cgcaatagcc
      181 ataggtgccc tcctcgatgc gagccaaagc cgcatcgatc ttggcgataa gctttcgctg
      241 gcggtctcga gcgcgaagct cgatggcgcg gtcggtttcc gacgacgccc gatcggcgat
      301 gtccggatga ttctcgctct cgctctgcaa ggccgccagg gtctcgcggc tctccttcaa
      361 gatcgcttct ttccaatccg acaatttgcg ccggaaatat tctttttggc gctcgttcat
      421 gaacggttcg tcttccgacg gtttgtaact ctcagtgata ccgaccactc ccattccgag
      481 aatcctccgc ctcaatgggc gcccttatag cgatgtcggc gccgccaaac aacgatttgt
      541 gcgccatccg acctcgcctc gccggccggg gcgggacttg atcaatcgcc ggccggcgcg
      601 catcctcgaa cggggtcagc ggccggactg gccggcgtgt aattggccaa gcggcccgtc
      661 agggcctggc ggagaagaag cgatgttcaa gcgcatcgtc gtccccatcg atctcgccga
      721 aatcgaactc gcgcagccgg cgatcgatca ggccgtcgaa ctggccgagg cgagcggcgg
      781 cgcgttgcgt ttggtgaacg tgcagccgct gttgccggcg accttcatgg actacgtgcc
      841 ggccgatttc gacgccgcgc aagaggagcg cgccgccaag gcgctcgaag agctcgccgc
      901 cggcgtgcgg ttgcccaagg agcgcatctc gcacgtggtg agggtcggcg gcgcctatca
      961 cgagattctc gccgaggcgc gcgcctgggc ggccgacctg atcgttgtcg ggtcgcaccg
     1021 gccggtcatg tccgattacc tgctcggctc caacgccaag accatcgttc ggcacgccca
     1081 ttgctcggtt ctggtcgtgc ggccttgagc gccgccgcgc tgtagccgcg gtcgccgacc
     1141 gcggctacag cagcctccta ccgcgtcagc tcccgcatcg cgccttcgag cccatccagc
     1201 gtcagcgcgt acatgcggtc gccgaaaatc cggcggatca tcgccgttga ctgcgaatag
     1261 ccccattgcg gctcgggcgt cgggttgatc caagcggcgt gcggataggc gcgcgtgacc
     1321 cgctcgagcc atacttgccc aggctcggga ttgacatgct cgaccgagcc gccgggcatg
     1381 gtgatctcat aggggctcat cgaggcgtcg ccgacgaaga tcgcgcggta gtcgcggcca
     1441 taggtgttga gaatgtcgag cgtcggcgtg cgctcctcgt ggcggcggcg gttcttcgtc
     1501 cacacgctct catagagaca attgtggaaa tagaaatgct gcagccgctt gaacgcgctg
     1561 cgcgccgccg cgaagaggtt ctcggcggcc tcgacatgcc aatccatcga gccgccgacg
     1621 tcgaggaaga gcaagacttt gatcgcgttg cgccgctctg gccgcaactg aacgtcgata
     1681 tagcccttct tggcgctgtc gctgatcgtc cgatcgagat cgagctcgtc gggcgcgccc
     1741 tcgcgggcga agcggcgcag ccgcctgagc gcgatcttca tggcgcgcgg accgagttct
     1801 tcgtcgccgt cgagatcctt gaagtcgcgc cggtcccaga ctttgacggc gcggccatgc
     1861 cggcccttgt cctggccgat gcgcacgcct tcgggatttt cgccataggc gccgaatggc
     1921 gacgttccgg ccgtgccgat ccatttcgag ccgccctgat ggcggccctt ctgctcggcg
     1981 aggcgctggc gcaaagtctc catcagcttg tcgaagccca aggcttttag ctcggcgcgc
     2041 tcttcgggcg tcagatagcg ctcgacgagc ttgcgcagcc attcttccgg tatctcgacc
     2101 cgctcgattg cggcggcgag cgagagcgcg cccttgaacg tcgcggcgaa gacgacgtcg
     2161 aacttgtcga gattgcgctc gtccttgacc aaaagcgcgc gcgagagatg gtagaaagcc
     2221 tcgaccgatt gttcggcgag gtcgcggtcc agcgcctgca tcaaggtcaa atattcgcgc
     2281 ggcgtgaccg gaacatgcgc ttgccgcagc gcagcgaaaa aggacaggaa catcgccgca
     2341 ttattgcatg gcggcgaaga gaatccatcg gcgccgccag atcggcaaat ctcgcgagaa
     2401 gcagaggaaa tatcgtcgca aggcttggaa acgccttggc agatgatcca gcggcggact
     2461 acgcttcggc aggaaacgcg caagttgcgg cgcgccgcgc cgcgggaggc ttcatgacca
     2521 gcctgaaatg gctcgccgcc ctgcccttca tcgcaatcct tggcggaacg gctttcttca
     2581 actcggtcga accgctggtg cttggcctgc cgctgattct cgcctggctg gttctgtggg
     2641 tgctgctgac cgcggcgatc atggcgctca tcttcttcaa cgacccggcc aattcggatc
     2701 gcgccgaaag cgacggccga tgaacagcgc gctgatcatc atctttctga ccgccgctct
     2761 ggcgctcgtc cttggcctgg tcgcgcgcag cggcaaggaa atgacgctcg agcagtggac
     2821 cgtcggcggt cgcggtttcg gcggcatcct ggtcttcctg ctgcaagccg gcgaaatcta
     2881 cacgaccttc acctttctcg gcggcagcgg ctgggcctat ggcataggcg cccagaccta
     2941 ttacatcctc gcctatggct gtctcgccta tatcctctct tattggctcc tgccgccggt
     3001 ctggcgctac gctcgccagc agcgaatcgt gtcgcaatcg catttcgtcg cgcgaaaata
     3061 cgacagcccg ctgctcggcg tgctggtcgc cgcggtcggc gtcgtcgcgc tggttccgta
     3121 cctcgtgctg caattcaaag ggctcggcat catcgtgtcg gtcgcgtcgt acggcgcgat
     3181 ttcgtcaacc gcggcgatct ggatcggcgc ggcgatcatc accgcttacg tcatggtctc
     3241 gggcgtgcgc ggctcggcct ggacgtccac agtgaaggat ttgatgatcc tggcggtcgt
     3301 ggtgtttctc ggcatttacc tgccgctgca ttactacggc ggctatggcg acatgttccg
     3361 ggcgatcgat gcggccaagc cgggcttcct ggcgctgaag ccttccggcg acggcggcct
     3421 gatctggttc caatcgacgg ctttgctctc ggcgctcggc ttctacatgt ggccgcacgc
     3481 tttcggcgcc gttttcaccg ccaaggccga gcatattttc cgccgcaacg cgatcatgct
     3541 gccgctctat caactgatcc tgctcttcgt ctttttctgc ggtttcgcgg cgatcttgaa
     3601 ggcgccgggc ctgaccggcc cggacatcga tctctcgctg ttcaagttat cgatcaacac
     3661 tttcgatcct tggtttgtcg gggtgatcgg cgcagccggc gtgctgaccg ccctcgtgcc
     3721 gggctcgatc attctcgtcg ccgcctcgac gctctgcgcc aatgacgtgc tgcgcggcct
     3781 caggcgggaa atgcgcgagg agacggtggc ttggacggcg cgaggcctcg tcccgatcgt
     3841 cgccttggtc gccgtcttct tcaccctgca aggcggcaag acgatcgtcg ccttgctgct
     3901 gatgggctat tccttcgtca cccagttctt ccccgcgatc gtctgcagcc tgatgcgcaa
     3961 caacccgctg accaaagccg gcgccttcgc cggcatcgct gtcggcgtcg cgaccgtcgt
     4021 cgcggtcacg ctcagtcaca gcacgctcgc gacgctcgcg ccgttcctgc cgaagccgat
     4081 tcaagagatc aatgtcggct tcttggcctt gatcttgaac gtaatcgtcg ccgctgtcgt
     4141 cagcgccggc gcgcgggcgg cgaccatcgc gagcgcggag cgggcttaag ggccgcctgg
     4201 aggcgcggtc gctgaccgcg ccgcttccgc cacgccgcgg tcgacgactg cggcgccaac
     4261 taacatgtta gcttcggaaa gagccaaacg tggagagccc cgcgatggcg cttcggcgcc
     4321 tccgcttgtc atgggaaggc gtttcgggtc atttggcccg cccagcgcgc ttgagcggcg
     4381 ccctcgattc gccgctttcg accaccttga gagttccgat gcgtctttcc cgctattttc
     4441 tgcccattct ccgcgagacg cccaaagagg cggaaatcgt ctcgcatcgc ctgatgctgc
     4501 gggccggcat ggtccgccag gaggcggccg gcatctacgc ctggctgccg ctcggcctgc
     4561 gcgttctggc gaaaatcacc gagatcgtgc gcgaggagca gaatcggtcc ggcgcgatcg
     4621 aattgctcat gccgaccatc cagtcagccg acctttggcg cgaatccggc cgctacgagg
     4681 attacggcaa ggaaatgctg cggatcaagg accggcacga gcgcgacatg ctttatgggc
     4741 cgaccaacga ggagatgatc accgagattt tccgcgccta tatccgctcg tacaaagacc
     4801 tgccgctcaa cctctatcac atccagtgga agttccgcga cgaggtcagg ccgcgcttcg
     4861 gcgtgatgcg ctcgcgcgaa ttcctgatga aggacgccta ttcgttcgat ctcgacgccg
     4921 aaggcgcccg tcattcctac aacaagatgt tcgtcgccta tctgcgcacc tttcttcgcc
     4981 tcggcctcac cgcgatcccg atggtcgccg ataccggccc gatcggcgga aacctgagcc
     5041 atgaattcat catcctcgcc acgaccggcg agagcgaaat ctattgccac aaggattatc
     5101 tgagcttccc gccgccgccc gagacgatcg atttcgatga ttccgctgcg gtgcaggcgg
     5161 tcgtcgacaa atggacgtcg ctttacgccg caacctcgga aaagcacgat gcggcggctt
     5221 tcgccgccct gccggaaacg gcgcggcttt cggcgcgcgg catcgaagtc ggccatattt
     5281 tctacttcgg gacgaaatat tccgagccga tgaaagcggt ggtcaatggc ccggacggta
     5341 aggagcacgc cattcacggc ggctcctatg gcatcggccc gagccgccta gccgcggcct
     5401 tgatcgaggc caaccacgac gaagccggaa tcatctggcc ggacgcggtt gcgccgttcc
     5461 gcgtcggact cgccaatctc aaagtcggcg acagcgccac cgacgccgcc tgcggccgca
     5521 tccacgccgc tctggaggcc gcgggaatcg acgtgctcta tgacgatcgc gacgagcggc
     5581 cgggggccaa attcgcgacc ttggacttga tcggcctgcc gcatcagatc ctggtcgggc
     5641 cgcgcagttt ggccgagggc aaggtggagc tgaaagagcg ccgcagcggc gcgcgcgacc
     5701 tcgtcagcgt cgaggacgcg atccgccgct tcggcggcca gacggagcgc ggatgaaagg
     5761 gcgcgacacg cggcgcggcg gcgccccttc tccccgcaag gcggggagaa ggtggccgtg
     5821 cgcagcacgg ccggatgagg ggctgcgccg gcctcaaagg aggcgcgccc gcccaagcgc
     5881 tcaccggcca gttcagcgcc gcccctcacc cggccgcttc gcggccaccc tctcgccgct
     5941 gcgcggggcg aggggagtcg cgcggcgaga gggagagcgc tcatgaccgc cgccgcgctc
     6001 gacgttccga cagagaccgc tgccccgtcg ctcgccgccc gtcctttcgc ccgcttcgaa
     6061 tggatggtgg cgtttcgcta tcttcgcgcg cggcgcaagc acggctcgat ctcggtgatc
     6121 gccggcttct cctttctcgg catcgtgctc ggcgtcgccg cgctcatcgt cgtcatgtcg
     6181 gtaatgaacg gctttcgcca tgacctgctc gacaagatta tcggcctcaa cggccatctc
     6241 ttcctccagc ccgccgagac ttcgctcacc gattacgacg cggtgacgga gcgggtgaga
     6301 aaagtacaag gcgtcaagct cgccctgccg ctcgtcgaag gccaggcctt ggcgtcctcc
     6361 cagttcaact cgtcgggcgt gctggtgcgc ggcgtgcgcg aagcggatct gaagcaattg
     6421 ccggggattg ccggccatat caatctcggc acgctcaacg gcttcgacag cgccggaggc
     6481 gcggcgatcg gcgcccgtct cgcggagcat ttgggcctgc gcatcggcga caagatcacc
     6541 atcatcgcgc cgcgcggcgc gcaaacgccg ttcggcgtca cgccgcgcat caagtcctat
     6601 ccggtcgtgg cgatcttcca gatcggcatg ttccagttcg acaacacctt cgtcttcctg
     6661 ccgctgccgg aagcccaggc gttcttcaat ctcgacggcc aggtcaatgt gatcgaggcc
     6721 tttgtcgacg atcccgaccg catggacgag atgcgccagc ggatcgagcc cgccttgcag
     6781 cggccgatga tcatgatcga ctggcgccag cgcgaccaga ccttcttcga ggcgctgaat
     6841 gtcgagcgca acgtgatgtt cgcgatcctg acgctgatcg tcctcgtcgc ggcgctcaac
     6901 atcatttccg gcctgatcat gctggtgcag gacaaggcgc gcgacatcgc cgtcttgcgc
     6961 acaatgggcg cgacgcgcgg cgcggtgatg cgcatcttcc tgatcaccgg cgcgacgatc
     7021 ggcgtcgtcg gcacgcttgt cggcgtgctg ctcgggctgc tggtcgcgca aaatgtcgag
     7081 gcgatccgcc acgggctcaa ctggctgctc aacgccaatc tctttccggc cgaagcttat
     7141 ttcctctcgc atttgccgtc gcgcgtcgag cccggcgacg tgattgcggt ggtggtcatg
     7201 acgctcgctt tatcgctcgc cgcgacgctc tatccgtcct ggcgggcggc gcggctcgat
     7261 ccggtcgagg cgctgcgcta tgaatgaggc ggcgacgccg gcgctctggc ttcacggggt
     7321 cgagaagcgc ttcggccgcg gcgagacgct gctggaaatc ctgcgcggcg tcgatctcgc
     7381 tctatggtcc ggccaatcgg tggcgctgat cgcgccgtcc ggcgccggca aatcgacctt
     7441 gctgcatgtc gccggattat tagagacacc cgatgccggc gaagtcttta tcggcggggc
     7501 gggaaccgcc ggcatgtcgg acgatcagcg caccgctttg cggcgcaacg agatcggctt
     7561 cgtctatcaa ttccatcacc tgctgccgga attctccgcc gccgagaatc tcatcctgcc
     7621 gcagatgatc cgcggcctgt cgcgcgcgga ggccggcaag cgcgccagcg aattgctcgc
     7681 ctatctcggc cttggcgcgc gcgagcgcca ccgcccggcc gaactgtccg gcggcgagca
     7741 gcagcgcgtc gcgatcgcga gagcggtcgc caatggcccg cgcctcctgc tcgccgacga
     7801 accgaccggc aatctcgatc ccaaaaccgc cgatcatgtc ttcggaacgc tgacggcttt
     7861 ggtcaaagcg agcggccttg ccgcgctgat cgcgacgcac aatctcgacc tcgcggcgcg
     7921 catggatcgc cgcgtgacgc tgcaagacgg caggctcgtc gaactcgcat aaaataaaac
     7981 gcggcgccgg tcggcgccgc gccgcaagct cgcttgcaat ggcggtcagt ggtgatggta
     8041 gtggccgcca tgccagccgc cgaaatggtg gaagtggtgg tagccatgcc acccgccgcc
     8101 ccatccgcca tagacggggc cgccataggt ataggccggg ccgccgtaat aatagccgca
     8161 accgccgtaa tagcagtcgt catagtcgtt gtagaagctg ttggccatcg ccatgccgat
     8221 gatcccgagc gccgcgcccg cgacggcggc gccggcgccg ttgtaataat ggtggcgata
     8281 gtgatagtga tggcggtacg cgacggtctc gaccggcgca gccgtttttt cgcttgccgc
     8341 gacgccgagc ggcccggctg tcgcgaccgg cccggccata gccggcgcaa tggcggcgag
     8401 cgacgccgcg aggctcgcgg caagggcggt cgaacggaaa aagctcatag ccatgacatc
     8461 ctccttgttc gggacgtcag cctggacggt ccctctgata ttgccccgcc cgctcccgct
     8521 tgacgtatgt tcgccggccg cgccggcaat ggcgcgctct tcaaattccg cgcacatttt
     8581 gtaaaattcg cgcgatgacg cgtcaggccg acggcctcgc ctcaatcacc acgaaagcct
     8641 gggcgagcgg atgctcgtcg gtgatcgtca catggatata ggcggcgcag ccggccggaa
     8701 tgatcgcggc gagccgcgcc gcggcgccgc cggtcatgtg catggtcggc tggccgctcg
     8761 gcagattgac gacgcccatg tcgcgccaaa acacgccgcg ccttagcccg gtgccgagcg
     8821 ccttggcgca ggcctccttg gcggcgaagc gcttggcgta ggaggcggcg cgatccttgc
     8881 ggccttccga tttgcggcgc tcgatctcgg tgaagcagcg ctggacgaac cgctcgccat
     8941 agcgctcgag cgtctcctcg atccggcgga tgtcgcaaag atcgctgccg atgccgatga
     9001 tcatggcgcg agctcggcgg cggaagctgg ggcggtacga cctttcgtgt caaggcgcgg
     9061 cgccaggggc ccctctcccc gcaaagcggg gagaggtacg gagaggggcc ggtcgcttag
     9121 cctcggctgc ggagttctac cccccgcgcc cctctccgta cctctccccg ttccgcttcg
     9181 cttcacgggg agaggagccc caagcgccgc ggcaaaacgc cggcggccag cgctcgccgt
     9241 ggaagcaagg ccgccatggt cgttctctag ccgcggcgcc gggaggggcc ggtcgcttag
     9301 cttcgaatcc tgagttctgc cccccgcgcc cctctccgta cctctccccg ttccgcttcg
     9361 cttcacggg
//
LOCUS       DATGJW010000284         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_884285, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000284 DATGJW010000000
VERSION     DATGJW010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_884285"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..2065
                     /locus_tag="VKU03_08760"
     CDS             8..2065
                     /locus_tag="VKU03_08760"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase"
                     /protein_id="HLJ71394.1"
                     /translation="MAIRERPISSALNEAQASAVEHGLGDPAGPAPLLVVAGAGAGKT
                     NMLAHRVARLILDGADPKRIMLATFSRRAAAELNGRVRRLLAKKLSDGAAEAAVPAYS
                     GTFHSIGARLLRDYAERLGLEPGFTIHDREDSADLLNLVRHELKLSEHQERFPTKGTC
                     LAIYSRTVNAKTELAETLGRHFPWCAAHEKTLRSLFAGYVEAKQRQSVLDYDDLLLYF
                     VQMMQIAPIAAEIAGRFDHLLVDEYQDTNRLQHEIVLALRPKGRGLTVVGDDAQAIYS
                     FRAATVRNILDFPKDFDPPARIVTLDRNYRSTKAILDASNAVIALSPQRFAKDLWTDR
                     AGGARPVLATVGEEIDQARYVATRVLAAREAGANLKSQAVLFRTAHHSAALELELTRR
                     NIPFVKYGGLKFLDAAHVKDALAMLRFAQNPRDRIAGFRVALTLPGVGPASAERIVQA
                     AEQEGLAALADWPAPARAAADFKSFAEILRDLAAGGEWPADLNRVVEWLRPHIERRYD
                     DFSARLADLDALERIGATYPSRERFLSELTLDPPDATSDEAGPPHRDEDFLILSTIHS
                     AKGQEWRSVFVLNAVDGCIPSELATGSRDEIEEERRLLYVAMTRARDELTLIVPQRFY
                     VHNQPRSGDKHVYASRTRFIPPKLLGFFDHVAWPPPQREPGPARAEGPRVDLAEKMRA
                     MWR"
     gene            2268..>2841
                     /locus_tag="VKU03_08765"
     CDS             2268..>2841
                     /locus_tag="VKU03_08765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71395.1"
                     /translation="MERHKAVSQTRIDGRAAFACAAYLIGVGLAFLLERIGAPDGLVR
                     VLGPVLALAAIVLIGALTRTTRIAAFYAADHSGAPRYGAAAYAAIAASLVACAAQDGG
                     LAAPRLAALAVGFVLAALVVAPLARGAGATSLRDVVSARYKILPLRLVLAGVCFATGV
                     LIAAAGYEVAADTLPGLLGLSRDGAIAAIGV"
BASE COUNT          410 a         1044 c          921 g          466 t
ORIGIN      
        1 ggcggccata gcgattcgcg agcgtccgat atcgagcgcc ttgaacgagg cgcaagcgag
       61 cgcggtcgag catgggctcg gcgatccggc cggccccgcc ccgctcctcg tcgtcgccgg
      121 cgccggggcc ggcaagacca acatgctcgc ccatcgcgtc gcgcggctga tcctcgacgg
      181 ggccgatccc aagcgcatca tgctcgcgac cttctcgcgg cgggcggcgg ccgagctcaa
      241 tggccgcgtc cgccgcctct tggccaagaa gctctcggat ggcgccgccg aggcggcggt
      301 cccggcctat agcggcacgt tccattcgat cggcgcgcgg ctcttgcgcg attatgccga
      361 gcggctcggt ctcgaaccgg gcttcaccat tcacgaccgc gaggactcgg ccgatcttct
      421 caatcttgtc cgccacgagc tcaagctttc cgagcatcag gagcgctttc cgaccaaggg
      481 gacgtgcctc gccatctatt cgcgcacggt caacgccaag accgagctcg ccgagacgct
      541 cgggcggcat tttccctggt gcgcggcgca tgaaaagacg ctgcgcagcc tttttgccgg
      601 gtacgtcgaa gcgaagcagc gccaaagcgt gctcgattac gacgacctgc tgctctattt
      661 cgtgcagatg atgcagatcg cgccgatcgc cgccgagatc gccggccgct tcgatcatct
      721 gctcgtcgac gaataccagg acaccaatcg gctgcagcac gagatcgtgc tcgccttgcg
      781 gccaaagggg cgcggcctca cggttgtcgg cgacgacgcg caggcgatct attcgttccg
      841 cgccgcgacg gtccgcaaca tcctcgactt cccgaaggat ttcgatccgc cggcgcgcat
      901 cgtcacgctc gaccggaact accgctcgac caaggcgatt ctcgacgcat ccaacgcggt
      961 catcgccctc tcgccgcagc gcttcgctaa agatctgtgg accgatcgcg ccggcggcgc
     1021 ccgtcccgtc ctggcgacgg tcggcgaaga gatcgaccag gcgcgctatg tcgcaacgcg
     1081 cgtcctcgcc gcccgcgaag ccggcgcaaa cttgaagtcg caagccgtac tgttccgcac
     1141 cgcccatcac agcgccgccc tggagctgga gctgacccgg cgcaacattc ccttcgtcaa
     1201 atatggcggc ctgaaatttc tcgacgcggc ccatgtcaag gacgcgctcg ccatgctgcg
     1261 cttcgcgcaa aatccgcgcg accgcatcgc cggcttccgc gtcgcgctga ccctgcctgg
     1321 cgtcggcccg gccagcgccg agcggattgt ccaggcggcc gagcaggaag gcctggcggc
     1381 gctggccgat tggcccgcgc cggcgcgagc ggcggcggat ttcaaatcgt tcgccgaaat
     1441 cctgcgcgac cttgccgcgg gcggcgaatg gccggcggac ctcaaccgcg tcgtggaatg
     1501 gctgcgcccg catatcgagc gccgctacga cgatttttcc gcccgcctcg ccgatctcga
     1561 cgcgctcgag cggatcggcg cgacctaccc ttcgcgcgag cgctttcttt ccgagctgac
     1621 gctcgatccg cccgacgcga ccagcgacga ggccggcccg ccgcatcgcg acgaggactt
     1681 tctcatcctc tcgacaatcc attcggccaa gggccaggaa tggcgctcgg tcttcgtcct
     1741 caacgccgtc gacggctgca ttccctccga gctcgccacc ggctcgcgcg acgagatcga
     1801 agaggagcgc cggctgcttt acgtggcgat gacccgcgcc cgcgacgagc tgaccttgat
     1861 cgtcccgcag cgcttctatg tgcacaacca gccgcgctca ggcgacaagc atgtctacgc
     1921 cagccgcacc cgcttcatcc cgccgaaact gctcgggttc ttcgaccacg tcgcctggcc
     1981 gccgccgcag cgcgagccgg gtcccgcccg cgccgaaggc ccgcgcgtcg accttgccga
     2041 gaagatgcgc gcgatgtggc ggtgaaagcc tggcgcacgg atcaaagggg tacggccttc
     2101 ttggaaattg acgcgcaagt acgtaccttc cagaatcccc gccgcgttac ccctcgctgc
     2161 tgacgcctcg cccctcgcga gagggggacg ccggttcggc cggctgaagg cggttgcggc
     2221 gcgcgccggc ttcgccgaat atcgactcgg cgcaggcgag aggcggcatg gagcggcaca
     2281 aggcggtttc gcagacccgg atcgacggcc gcgcggcctt cgcctgcgcc gcctatttga
     2341 tcggcgtcgg cctggcgttt ctccttgagc ggattggcgc gccggacggc ttggtgcgcg
     2401 tgctcgggcc ggtcctcgcc ttggccgcga tcgttctcat cggcgcgctg acgcgcacga
     2461 cccgcatcgc cgccttttac gcggccgatc attccggcgc gccgcgctac ggcgcggcgg
     2521 catacgctgc gatcgcggcg agcctcgtcg cctgcgccgc gcaagacggc ggactcgccg
     2581 ctccgcgcct cgcggctttg gcggtcggct tcgttctcgc ggcgctcgtc gtcgcgccgc
     2641 tggcgcgcgg cgccggcgcc acgtcgctgc gcgacgtcgt ctcggcgcgc tacaagatcc
     2701 tgccgctgcg gctggttctc gccggcgtct gtttcgcgac cggcgtcctg atcgccgcgg
     2761 ccggatacga ggtcgcggcc gatacgctgc cgggcctgct cggcctgtcg cgcgacggcg
     2821 cgatcgcggc gatcggcgtg a
//
LOCUS       DATGJW010000285         5667 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1403070, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000285 DATGJW010000000
VERSION     DATGJW010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5667)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5667)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5667
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1403070"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..309)
                     /locus_tag="VKU03_08770"
     CDS             complement(<1..309)
                     /locus_tag="VKU03_08770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004676885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDSL-type esterase/lipase family protein"
                     /protein_id="HLJ71396.1"
                     /translation="MAFCGFLLALVFSAAPAAARTLHIVALGDSLTAGLGLPAGQSFA
                     AVLQQKLRAKGYDVAVENAGVSGDIAADGLARLDWATPDGTDAVIVELGANDMLRGLD
                     P"
     gene            560..1255
                     /locus_tag="VKU03_08775"
     CDS             560..1255
                     /locus_tag="VKU03_08775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71397.1"
                     /translation="MADPAIEIRDLDLSLGRGAARVHILQGISLTIARGEAVGLIGPS
                     GSGKSTLLMTLAGLERPDRGDIVVDGVRLAGMSEDALAKFRGGRIGIVFQSFHLIPTM
                     TALENVAAPLELAGAADAFDRAEAELAAVGLAGRFAHYPAQLSGGEQQRVALARALAP
                     RPAILVADEPTGNLDEATGQAIIDLLFELRAERGATLVLVTHDPDLAARCDRTIRLRS
                     GRIEALAPARALA"
     gene            1443..3986
                     /locus_tag="VKU03_08780"
     CDS             1443..3986
                     /locus_tag="VKU03_08780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsX-like permease family protein"
                     /protein_id="HLJ71398.1"
                     /translation="MTVALFRLAFRDLRGGLAGLRIFLACVALGVATIVGVNSLARSL
                     ADGLSRDGRAILGGDAAFSLIHRQLSPAERQYLAARGALSEVATMRAMARNDAGDAAL
                     VEIKAVDSSWPAIGRAELAPDMTPQAALAQKDGVFGAAVEDAALDRLNVKIGDTLAIG
                     AIKVQIRAALESEPDRLGSGLGLAPRVLVSLAALDQTGLVQPGSLVRWTTRVVMNPGG
                     EPPSESAVKAFLAAAKKTFPEAGWEAVSRGNISPAFSKNLDRFTEFLALIGLTSLIVG
                     GVGVANAAQGFVERKRATLATLKAIGAAGGEVVAVALIEFLAVALIGVAAGLALGAAL
                     PFFVNGLFGAIIPFPLAPAVFPSELALGAAYGLLTAFVFAVAPLGRAHDLPVSALFRD
                     LVEPGRAWPRRRYVALAIAAAAAFAGLAIGMSAERRLAIIVVVATIVGFLALRLVALA
                     VMALARRAPRLSGLEWRWALANIHRPGALTPSVVLSLGLGLAVLVALTLIDNVLRGEI
                     GHATPSETPSFYFLDIRSADIGNFRAFLRREAPSAKIVEAPMMRGRIVKIGDRPAEGF
                     KAKDNVAWVLEGDRGVTYSDRPPEGSTVVKGEWWPADYSGPPLVSLEEEIADGLGLKI
                     GDAVTVNVLGRNITAKIANLRKVNWRSFAMNFVLVYSPNTFKGAPHTFLVTAAFPHDI
                     GQAAELAILRDAARDFPTVASLRVRDALRAFAQLLDKLALAIRSASGVALSTSVLVLA
                     GALAANRRSRIYDSVILKTLGATRGRLTAAFLIEYALLGAAASIFGLAAGAAAASLIV
                     TKVMALDFAFAWAPALEAALGALVFTIALGLIGSWRILGQKPAAILREL"
     gene            4105..4338
                     /locus_tag="VKU03_08785"
     CDS             4105..4338
                     /locus_tag="VKU03_08785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71399.1"
                     /translation="MRFLLAAACAAGLLFSVSSAQAVGCLSGAVAGGVAGHYAHHHGV
                     IGAMAGCAYGHHLHKEQQRKRAEQEKEKNHAAQ"
     gene            complement(4351..5088)
                     /locus_tag="VKU03_08790"
     CDS             complement(4351..5088)
                     /locus_tag="VKU03_08790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLJ71400.1"
                     /translation="MSPDINEFRSFYASQLGEIARRLIGRVIRARWENCVGQSIMGLG
                     YAAPYLDRFREEALRTLAFMPAEQGVVNWPSSGRSSSALVEPDMLPLPDAAIDRVLVI
                     HALEAAEHPNALLEEVWRVLTPGGRAILVTPSRSGFWARGDGTPFGFGQPYSKGQLRQ
                     LMRETLFSPIHWTEALYAPPFRRSFFIRSAPAIERAGAAIGMPFAGVYIVEATKQLYR
                     PVALRRSLRRALPRLSPALAPGAHPVN"
     gene            complement(5228..5665)
                     /gene="aac(6')"
                     /locus_tag="VKU03_08795"
     CDS             complement(5228..5665)
                     /gene="aac(6')"
                     /locus_tag="VKU03_08795"
                     /EC_number="2.3.1.82"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004430089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047663 - aminoglycoside
                     6'-N-acetyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoglycoside 6'-N-acetyltransferase"
                     /protein_id="HLJ71401.1"
                     /translation="MEKCAPSDCDDWFALRAALWPEEPRDELRAQKEELIAKGESAAI
                     FLARDRDGAVVGIAEATLRRDFVNGCTTAPVAFLEGLYVAPAWRRRGVGRRLCAAVES
                     WGRELGCTELGSDTYLDNVGSQKMHEALGFEEMERVVCYRKII"
BASE COUNT          843 a         2002 c         1874 g          948 t
ORIGIN      
        1 cggatcgagg ccgcgcagca tatcgttggc gccgagctcg acgataaccg cgtccgtccc
       61 gtcaggcgtc gcccaatcga gccgcgccag cccgtccgcc gcgatgtcgc cggaaacgcc
      121 ggcattctcc accgcgacat cgtagccctt ggcgcgcagc ttttgttgca gcacagcggc
      181 gaagctctgc ccggccggca ggccgaggcc ggcggtcagg ctatcgccga gcgcgacgat
      241 atgcagcgtg cgcgccgcgg cgggcgcggc ggagaaaact agcgctaaaa gaaatccgca
      301 aaacgccaag acgcgcggcg aaacccttct ccccgcatcg cggggagaag gtggcccgaa
      361 gggccggatg aggggcagcg ccgaactcgc gaagttaagc gcccttcgaa gcgctaatct
      421 ccaagcgccg cgcggctccg tcgcccccct ctccccgcaa gcgggaagaa ggggatcgcc
      481 gctttgggag gcgaggtgga cgaaaagccg gcaatcccca tatgtgggcg caaggcgcct
      541 catcgaaagc aatccctcaa tggccgatcc cgcgatcgaa attcgcgacc ttgacctcag
      601 tttgggccgc ggcgcggccc gcgtccatat cctgcaaggg atcagcctga cgatcgcgcg
      661 cggcgaggcg gtcggcctga tcggcccgtc cggctcgggc aaatcgaccc ttctcatgac
      721 gcttgccggc ctcgaacggc cggaccgagg cgacatcgtc gttgacggcg tcaggctcgc
      781 cggcatgagc gaggacgctc tggcgaaatt tcgcggcggg cgcatcggca tcgtcttcca
      841 gtcattccat ctcattccga ccatgacggc gctggagaac gtcgccgcgc cgttggagct
      901 cgccggcgcg gccgacgcgt tcgaccgcgc cgaggccgag ctcgccgcgg tcggccttgc
      961 cggtcgcttc gctcattatc cggcgcagct ttcgggcggc gagcaacagc gcgtcgcgct
     1021 cgcccgagct ttggcgccgc ggccggccat cctcgtcgcc gacgaaccga ccggcaatct
     1081 cgacgaagcg acgggccaag cgatcatcga tcttctcttc gaattgcgcg ccgagcgcgg
     1141 cgcgacgctc gtcctcgtca cccatgatcc ggacctcgcc gcgcgctgcg accggacgat
     1201 ccgcctgcgc tccgggcgaa tcgaagcgct agcgccggcg cgagccttgg cgtgagtggg
     1261 catctttcat tggccggcgc aggcgctagc gccgcccccg cccgtaccct cccccgcttc
     1321 gcgggagagg gagccccaag cgtcgcgttt caacttttgc cgtcctgcca cgcgcaggca
     1381 tgctccgcgg catgggatcc ccctctcccc cgaagcggga gagggtacgg gagggggcct
     1441 gcgtgaccgt cgcgctcttt cgcctcgcct ttcgcgatct gcgcggcggt ttggccggct
     1501 tgcgcatctt tctcgcttgc gtcgcgctcg gcgtcgcgac gattgtcggc gtcaattcct
     1561 tggcgcgcag cctcgccgac gggttgtcgc gtgacggccg cgcgatcctc ggcggcgacg
     1621 ccgctttttc gctgatccat cggcaattgt cgccggcgga gcgccagtat ctcgccgctc
     1681 gcggcgcgct ttccgaggtc gcgacgatgc gagccatggc gcgtaacgac gcgggcgacg
     1741 cggcgctggt cgaaatcaag gccgtcgatt cgtcctggcc ggcaatcggc cgggcggaat
     1801 tggcgcccga catgacgccg caagccgcgc ttgcgcaaaa ggacggcgtg ttcggcgccg
     1861 ccgtcgagga cgccgcgctc gatcggctca acgtgaagat cggcgatacg ctcgccatcg
     1921 gcgccatcaa agtacaaatt cgcgccgccc tggaatccga gcccgaccgc ctcggctccg
     1981 gtctcggcct cgcgccgcgc gtgctcgtct cgctcgccgc gctcgaccaa accggcctcg
     2041 tccagccggg ctcgctggtg cgctggacga cgcgcgtcgt catgaatccc ggcggcgagc
     2101 cgccgagcga aagcgcggtt aaggcctttc tcgccgccgc caagaagact ttccccgagg
     2161 cgggctggga ggcggtcagc cgcggcaata tctcgccggc cttctccaag aatctcgacc
     2221 gcttcaccga gtttctcgcc ttgatcggct tgacctcgct gatcgtcggc ggcgtcggcg
     2281 tcgccaatgc ggcgcaaggc tttgtcgaac gcaaaagggc gaccctggcg acgttgaaag
     2341 caatcggcgc ggccggcggc gaagtcgtcg ccgtcgcgct gatcgagttt ctcgccgtgg
     2401 cgctgatcgg cgtcgccgcc ggcctcgcgc tcggcgccgc cctgcccttc ttcgtcaacg
     2461 gcctgttcgg cgcgatcatt cccttcccgc tggcgccggc ggtctttcca agcgagctcg
     2521 cgctcggcgc ggcctatggc ttgctgaccg ccttcgtctt cgccgtcgcg ccgctcggcc
     2581 gcgcccatga tttgccggtc tcggctctgt ttcgcgatct cgtcgagccc ggccgcgcct
     2641 ggccgcggcg gcgctatgtc gcgctggcga tcgccgccgc cgccgctttc gccggcctcg
     2701 ccatcggcat gagcgcggag cggcgcctgg cgatcatcgt cgtcgtcgcg acgatcgtgg
     2761 gctttctggc cttgcggctc gtcgcgctcg ccgtcatggc tttggcgcgg cgcgcgccgc
     2821 gcctcagcgg tctcgaatgg cgctgggcgc tcgccaatat tcaccgtccc ggcgcgctga
     2881 cgccctcggt cgtattgtcg ctcggcctcg gcctcgccgt gctggtcgcc ctgacgctga
     2941 tcgacaatgt cctgcgcggc gagatcggcc atgcgacgcc gagcgagacg ccgagcttct
     3001 actttctcga catacgcagc gccgatatcg gcaatttccg cgcctttctg cgccgcgagg
     3061 cgccgtcggc gaagatcgtc gaagcgccga tgatgcgcgg gcgcatcgtc aagatcggcg
     3121 accggccggc ggagggcttc aaggcgaaag acaatgtcgc ctgggtgctg gagggcgacc
     3181 gcggcgtgac ctatagcgat cggccgccgg aaggctcgac tgtcgtcaag ggcgaatggt
     3241 ggccggccga ttattccggg ccgcctctgg tctcgctgga ggaggagatc gccgacgggc
     3301 tcggcctgaa aatcggcgac gccgtcacgg tgaatgtgct tgggcgcaac atcacggcga
     3361 agatcgccaa tctgcgcaaa gtcaattggc gcagcttcgc gatgaatttc gtgctcgtct
     3421 attcgccgaa cacgttcaag ggcgcgccgc atacgttcct cgtcaccgcg gcttttccgc
     3481 acgacatcgg ccaggcggcc gagcttgcga tcctgcgcga cgcggcgcgc gactttccga
     3541 ccgtcgcctc cttgcgggtg cgcgacgcgt tgcgcgcttt cgcgcaattg ctcgacaagc
     3601 tcgcgctcgc catccgctcg gcctccggcg tcgcgctttc gacctcggtg ctggttttgg
     3661 ccggcgcgct cgccgccaat cggcgcagcc gcatctatga ttccgtcatc ctcaagacgc
     3721 tcggcgcgac ccgcggccgg ctgaccgccg ccttcctgat cgaatacgcc ctgctcggcg
     3781 ccgccgcctc gattttcggc ttagcggccg gcgccgccgc cgcctccttg atcgtgacca
     3841 aggtcatggc gctcgatttc gctttcgcct gggcgccggc gcttgaggcc gctctcggcg
     3901 cgctcgtctt cacgatcgcc cttggcttga tcggctcatg gcggattttg ggccaaaagc
     3961 cggccgcgat tctcagggag ttgtaacggc gccggccagc gcctaactaa cgtcaacgcc
     4021 tgcccgcatc ggtaaaacta aacaaatcgc cgggcggcgc gttattgtcg ctctcgacca
     4081 tcgtcagcaa aaggaccgtg acccatgcgc ttcttactcg ccgccgcttg cgccgccgga
     4141 ttgcttttct cggtcagcag cgcccaagcc gtcggctgcc tttcgggcgc ggtggcgggc
     4201 ggcgtcgccg gccactacgc ccaccatcac ggcgtcatcg gcgccatggc cggctgcgcc
     4261 tatgggcatc acctccacaa ggagcagcag cgcaagagag ccgagcagga aaaggagaag
     4321 aatcacgccg cccaataggg cggcgccggc tcagttgacg ggatgcgcgc ccggcgccag
     4381 agccgggctc aatctcggca gcgcccggcg caggctgcgg cgcaacgcga ccggccggta
     4441 gagctgcttg gtcgcttcga cgatatagac gccggcgaaa ggcatgccga tcgccgcgcc
     4501 ggcgcgctcg atcgccggcg ccgaacggat gaagaagctg cgccggaacg gcggcgcata
     4561 gagcgcttcg gtccaatgga tcggcgagaa cagggtttcg cgcatcagtt ggcgcaattg
     4621 ccctttcgaa tagggctggc cgaagccgaa cggcgtgccg tcgccgcgcg cccagaagcc
     4681 gctgcgcgac ggcgtgacca ggatcgcccg gccgcccggc gtcaagaccc gccagacttc
     4741 ttcgagcagg gcgttgggat gctcggcggc ctcgagcgcg tggatcacca gcacgcgatc
     4801 gatggcggcg tcgggcaagg gcagcatgtc cggctcgacc agggccgacg agctgcggcc
     4861 cgaggacggc cagttcacca cgccctgctc ggccggcatg aaggcgagcg tgcgcagcgc
     4921 ctcctcgcga aagcggtcga gatacggcgc ggcgtagccg agccccatga tcgattggcc
     4981 gacgcagttt tcccagcgcg cgcggatgac gcgtccgatc agcctgcgcg caatctcgcc
     5041 gagctgcgag gcgtagaagc tgcgaaattc gttgatgtcc ggcgacatgg cggaattttg
     5101 ccacgccccg gcgcacgggg gaagagcgcg tcattcccgc gcaagcggga agccaggacc
     5161 gccgttgccg caagcgcggc gactgctgga ttcccgcttt gcgcgggaat gacaacgtac
     5221 cggatgatca gatgatcttc cggtagcaga cgacgcgctc catctcctcg aagccgagcg
     5281 cttcatgcat cttttgcgat ccgacattgt cgaggtacgt gtcggagccg agctccgtgc
     5341 agccgagctc gcggccccag ctttcgaccg ccgcgcagag ccggcgtccc acgccgcggc
     5401 ggcgccaggc gggcgccacg tacagccctt ccagaaatgc gaccggcgcg gtcgtgcagc
     5461 cgttgacgaa gtcgcgcctc agcgtcgcct cggcgatgcc gaccaccgcg ccgtcccgat
     5521 cgcgggcgag aaagattgct gcgctctcgc ctttcgcgat caattcctcc ttttgcgcgc
     5581 gcagctcatc gcgcggctcc tcgggccaca gcgccgcgcg gagcgcgaac caatcgtcac
     5641 aatcggaagg cgcgcatttt tctatat
//
LOCUS       DATGJW010000286         2565 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_262865, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000286 DATGJW010000000
VERSION     DATGJW010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2565)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2565)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2565
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_262865"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VKU03_08800"
     CDS             complement(<1..208)
                     /locus_tag="VKU03_08800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HLJ71402.1"
                     /translation="MNHDSYPEDYIRGILNSVKRIAMVGASANPARPSYFVLKYLATR
                     GYEMLPINPGLAGKDINGIKVYGSL"
     gene            complement(246..1058)
                     /locus_tag="VKU03_08805"
     CDS             complement(246..1058)
                     /locus_tag="VKU03_08805"
                     /EC_number="4.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006020800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase"
                     /protein_id="HLJ71403.1"
                     /translation="MSARRAEAPASLLDHRVEGAAAILTLDSQASRNGLSLAMIEALQ
                     NALAAIAKDRKIRVVVLAARGPVFSSGHDLREIRAHRNDEDHGRAYDERLMARCAEMM
                     QAIVALPKPVIAAVEGVATAAGCQLVATCDLAIAGEKARFALPGVGIGLFCSTPLVAL
                     GRAISRKHAMEMALTGELYSARDAERFGLVNRVVSAGGAEEEAMKLAAAFAKRSAKTL
                     AIGKRTFYGQIEKPLDEAYVFAGRAMVENLAERDADEGISAFLDKRTPQWEE"
     gene            complement(1145..2065)
                     /locus_tag="VKU03_08810"
     CDS             complement(1145..2065)
                     /locus_tag="VKU03_08810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007561427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ71404.1"
                     /translation="MTTTPAPAPQHAKDSPHGSLAPLALLVSVGSFLGLTANVVKIAA
                     GAGWPPLAFLFWSALVAGFILLIAAASRGETPGRRGPDFAYYLICGLLSIALPNALGY
                     GAVMHVGASFVALCLALPPLLTYGLALLLNMEKLRATRALGILTGLAGAIVLGVSKAK
                     GSLADPIWIIGALASPVFIALGNIYRTLRWPPGASALSLAPGMLIFGSLLIAPILPLA
                     GVSFAPTAFTSAVVWLLVAEIAIFSMTYALYFVLQKLAGPVYLSQIGSVAAIFGAALA
                     VLALGEPANTGLLIAAALILAGVVLVNRRR"
BASE COUNT          517 a          749 c          873 g          426 t
ORIGIN      
        1 taagcgagcc atagactttg atcccgttaa tgtccttgcc ggcgaggccg ggattgatcg
       61 gcagcatttc atagccgcgc gtcgcaaggt atttgagcac aaaataagac ggccgcgccg
      121 ggttggcgga cgcgccgacc atggcgatgc gcttcactga atttaggatg ccgcgaatat
      181 aatcttcggg gtacgagtcg tggttcatgt taatgacaaa gaactctgat ttcgaggccg
      241 catgctcact cctcccattg cggcgtgcgc ttatccaaga acgccgaaat cccctcgtcg
      301 gcgtcgcgct cggcgaggtt ttcgaccatg gcgcggccgg cgaagacgta ggcttcgtcc
      361 agaggcttct cgatctggcc gtagaaagtt cgcttgccga tcgcgagggt cttggcggag
      421 cgcttggcga aagcggcggc gagcttcatc gcttcttctt ccgcgccccc tgccgagacg
      481 acacgattga cgaggccgaa gcgctcggcg tcgcgcgcgg aatagagttc gccggtcagg
      541 gccatctcca tcgcgtgttt gcgcgagatg gcgcggccaa gggcgacaag cggtgtcgag
      601 cagaagagcc cgatgccgac gccgggcagc gcaaagcgcg ccttttcgcc ggcgatggcg
      661 agatcgcagg tcgcgacgag ctggcagccg gcggcggtcg cgacgccttc gacggcggcg
      721 atgaccggct tcggcagggc gacaatcgcc tgcatcatct cggcgcaacg cgccatgagg
      781 cgctcgtcat aggcgcggcc gtggtcctca tcattgcgat gggcgcggat ttcgcgcagg
      841 tcgtggccgg aggagaagac cggcccccgc gcagcgagga cgaccacgcg aatcttgcgg
      901 tccttggcga tggcggcgag agcgttttga agcgcttcga tcatcgccag cgacaaaccg
      961 ttccggctcg cttggctgtc gagcgtcaat atggcggcag cgccttcgac gcgatggtcg
     1021 agaagcgagg ctggggcttc ggcgcgtcga gctgacatta cttcgccaat gtcgtctgtc
     1081 ccgaccgaag gttcaagcgc cggaagcgcc gagctggcgc gcgcgaggcg cgggatcacc
     1141 gccctcaccg ccgccgattg accaaaacga cgccggcgag aatgagcgcg gccgcgatga
     1201 gaagcccggt attggcaggc tcgcccaagg ccaaaacggc caaagccgcg ccgaagatcg
     1261 ccgcgaccga gccgatctgg ctgaggtaga ccggcccggc gagcttttgc aggacaaagt
     1321 agagcgcata ggtcatcgag aagatcgcga tttcggcgac gagcagccag acaacggcgc
     1381 tggtgaaagc ggtcggggcg aaggagacgc cggcgagagg gaggatcggc gcgatcaaga
     1441 gcgatccgaa gatcaacatc cccggcgcca gcgacagcgc gctcgcgccg ggcggccagc
     1501 gtagggttcg gtagatattg ccgagagcga tgaagacggg gctcgccagg gcgccgatga
     1561 tccagatcgg gtcggcgaga ctgcccttcg cctttgagac gccgagaacg atcgcgccgg
     1621 cgaggccggt cagaattccg agggcgcgcg tcgcgcgcag cttctccatg ttgaggagca
     1681 aagcgaggcc gtaggtcagg agcggcggca aagcgagaca gagcgccacg aaactcgccc
     1741 cgacatgcat gacagcgcca taacccaagg cattgggcag agcgatcgag agcagaccgc
     1801 agatgagata ataggcaaag tccggcccgc ggcgccccgg cgtttcgccg cggctggcgg
     1861 cggcgatgag gagaatgaat cccgcgacca gcgccgacca aaaaagaaag gcgagcggcg
     1921 gccagccggc gccggcggcg attttcacga cattggcggt gagaccgagg aacgagccaa
     1981 cggagacgag cagcgccagg ggcgcgagcg agccgtgagg cgagtctttt gcatgctggg
     2041 gcgccggcgc cggcgtggtc gtcacagggg gtctctgatt tcggttagcg ttggcgttct
     2101 tagcgggaga atggcgatcg cgcgaacacg gtagggcggc tgtcccacgg cgcgcgcgga
     2161 cgcgccgaac ccgtaccggc ctcgcgcgag gcatcctatg cgtggctggc cggagccgcg
     2221 cacttcgacg ttgagcggcg cgcttaagaa gcgggcgaag gcgattgcag cggcacgtcc
     2281 gtggagcaat ggtcccggcc aagattagtt gaaacgccag tgcctgctga ggtatttaat
     2341 cattatatta taatatatta cacaatcatt ctaacgttct tctcaaccaa aaatattgac
     2401 cgcgaacgat tcgccctgat tctggctgtc gaaaggcgtg ggtcggccgg ctcgccgctc
     2461 gaaatttgca cccgtggaag cataatcgcg cttaacccat tgatattgct agatccggtc
     2521 ttttgcgata ttttcgatgt taacaatatc aatgggttac agcac
//
LOCUS       DATGJW010000287         4222 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_367960, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000287 DATGJW010000000
VERSION     DATGJW010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4222)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4222)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4222
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_367960"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..411)
                     /locus_tag="VKU03_08815"
     CDS             complement(<1..411)
                     /locus_tag="VKU03_08815"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLJ71405.1"
                     /translation="MTRLFEHRLIFVRHGETAYNLENRLQGQRDIALNGAGREQARAV
                     GRYLRKAMGATIDAIEARGQFWASPLERTRETMALARQAMGLKPDTYRIDARLKELTF
                     GAWEGMTWGEVWARDPAGAKAREEDKWRFAPPGGE"
     gene            622..1373
                     /locus_tag="VKU03_08820"
     CDS             join(622..952,952..1373)
                     /locus_tag="VKU03_08820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611079.1"
                     /ribosomal_slippage
                     /note="programmed frameshift; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS5 family transposase"
                     /protein_id="HLJ71406.1"
                     /translation="MDRFVLTDAQWARMEPLCLGKPGDPGRRGKDNRLFVEAVLWIAR
                     TGSPWRDLPPSFGHWNSVFTRFRDWVKADIWKRLFDAVSDDPDMEYATVDATIVKVHR
                     HGQGAKGGPQNQAIGRSKGGMTTKILALTDALGNLVRFVLLPGQRFDTVGVPPLIEGL
                     AFEALIADKAFDSNAIIAELDARGARVVISQHPRRAKPLAIDEEMYKWRHLIENFFGK
                     LKEFKRIAMRADKTDQSFASMIHLAAAVINSR"
     gene            complement(1387..2565)
                     /locus_tag="VKU03_08825"
     CDS             complement(1387..2565)
                     /locus_tag="VKU03_08825"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLJ71407.1"
                     /translation="MSAISLDAPTALALIAIIAATLGGLILFSWTQGRRNFYLALWGG
                     GDILAGIAAGLWFGRGVLPDFISINIATGLMALAYGIMFAAARDFAGKRTPLGLIAAG
                     ALLWLAACGYQPFYASTVSRTAMMSAVFAIYTAASAAEFWGGRAERLASRLPAVAVLF
                     IHAVACAFRIPVSLAGGLNSPRLAAGPWFTVFVFESLLHILAMAILIVSMTKERAELE
                     QRVVASTDDLTGAATRRAFLAGGDAKIGAAEKDGLDSSLLLFDLDHFKAINDSFGHEM
                     GDRALSAFADCVTKALRPGDLFGRLGGEEFAALLIGPSAETALAIAERMRRAVEAIKF
                     AEARGGLQISVSIGVASASRSQRRLGDMLREADRALYRAKSSGRNRVERHEAAPKLVA
                     "
     gene            2933..>4222
                     /locus_tag="VKU03_08830"
     CDS             2933..>4222
                     /locus_tag="VKU03_08830"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS-domain containing protein"
                     /protein_id="HLJ71408.1"
                     /translation="MRRTTRPQSGLARGATAEARRLIGAPAGAFLLLALATALSPRAA
                     LASQSFAPFLAVEPTRDNPAGLAVLIGLVLFATFTSILHLVGRRQWALREKALTAELS
                     QLQARLDRANLFLSSDQQIVVAWGSATGEPDVEGEYSLVTDAPLPRRVLGFGAWLAPE
                     PAQKLDQAVERLRQRGEGFSLTVASLAGRHFDIEGRAIAGRAVMRIRDISGDRLQLVR
                     LRENHNRLNEEIGALRAMLNALPSPAWTRDAHGRIAWVNAAYANAVEAKDAIDAAARG
                     LELFDPATREAALSALQAGETWRRRAPAVAAGERRLYDVVEVPTGAGAAGIANDLSNI
                     VALQGELESQIASHARTLDQLSTGVAIFDRAKRLVFHNAAYRQIWALDAVFLEQQPTD
                     DEILDRLRAKRQLPEQADFRSWKAQLLAAYQAIDPVEQ"
BASE COUNT          737 a         1432 c         1360 g          693 t
ORIGIN      
        1 ctcgccgccc ggcggcgcga agcgccattt gtcctcttcg cgcgctttcg cgccggccgg
       61 atcgcgcgcc cacacttcgc cccaggtcat tccctcccat gcgccgaacg tcagctcttt
      121 gagccgcgcg tcgatacgat acgtatccgg tttcaaaccc atcgcctgcc gcgcgagcgc
      181 catcgtctcg cgcgtacgtt cgagcggcga ggcccagaat tggccgcgcg cctcaatcgc
      241 gtcgatcgtc gcgcccatcg ccttgcgcag atagcgtccg acggcgcgcg cttgctcgcg
      301 gccggcgccg ttcagcgcga tgtcgcgctg accctgcagg cgattttcaa gattgtaggc
      361 ggtctcgcca tggcgcacga aaatcagccg gtgctcgaaa aggcgcgtca ttcggcgcct
      421 gcctcctatg gccgggcggc gatgtcaagc gggcgagagc aagctgctca tgtcattcat
      481 gcgaaagtgg aatccgggga ttcgccagct ttcggcagag aagtactgga ttcccgcttg
      541 cgggatgaca agcgctcgat ggcctagggt ctgttgggat tcaggattct catcctggtt
      601 tggttgtgat tcaagctgtg gatggaccga ttcgttttga ctgacgccca atgggcgagg
      661 atggagccgc tttgtcttgg caagccgggc gatcccggcc gccgcggcaa ggacaaccga
      721 ctgtttgtcg aggccgtgct gtggatcgcc cgaaccggca gcccctggcg ggatttgccg
      781 ccttcgttcg gccactggaa cagtgtcttc acgcgcttcc gcgactgggt aaaggcggac
      841 atctggaaac ggctgttcga cgccgtgtcc gacgatcccg acatggaata cgccacggtc
      901 gacgccacca tcgtcaaggt ccaccgccac ggacagggcg caaaaggggg actcaaaacc
      961 aagcgatcgg ccgctcgaaa ggcggcatga ccaccaaaat cctggcgctc accgacgcgc
     1021 tcggcaatct cgtgcgcttc gtgcttctgc cgggacagcg cttcgacacg gtgggcgtgc
     1081 cgccgctcat cgagggattg gccttcgagg cgctgatcgc cgacaaggcg ttcgatagca
     1141 acgccattat cgccgagctc gatgcgcgcg gcgccagggt cgtcatctcc cagcatccgc
     1201 gacgcgcaaa gcctctcgcc atcgacgagg agatgtacaa atggcggcat ctcatcgaga
     1261 acttcttcgg caagctcaag gagttcaaac gcatcgccat gcgcgccgac aagaccgatc
     1321 aaagtttcgc ctccatgatc catctggccg ctgccgtgat aaactcccga tgaatcccaa
     1381 cagaccctaa gcgaccagtt tgggcgcggc ttcgtgccgc tcgacgcgat tgcggccgct
     1441 ggacttcgcg cgatagagcg cgcggtcggc ttcccgcagc atgtcgccga gccgcctctg
     1501 cgagcgcgag gcgctggcga cgccgatgct gaccgagatt tgcaagccgc cccgcgcttc
     1561 cgcgaatttg atcgcctcga ccgcgcggcg catccgctcg gcaatggcca gcgcggtctc
     1621 ggcgctcggt ccgatcagca aagcggcgaa ctcctcgccg ccgagccggc cgaacagatc
     1681 gccgggacgc aacgcctttg ttacgcaatc ggcgaaggcg ctcagcgcgc gatcgcccat
     1741 ttcatggccg aaactatcgt tgatcgcctt gaaatgatcg agatcgaaaa ggagcagcga
     1801 cgaatcgagc ccgtccttct ccgccgcgcc gatcttggcg tcgccgccgg cgagaaaggc
     1861 gcggcgcgtc gccgcgccgg tcagatcgtc ggtcgaggcg acgacccgct gctcgagctc
     1921 ggcgcgctct ttcgtcatcg acacgatgag gatcgccatc gccagaatat ggagcagcga
     1981 ttcgaagacg aagacggtga accacggccc ggcggcgagg cgcggcgagt tcaggccgcc
     2041 ggccagcgaa accggaatgc gaaacgcgca agccacggcg tggatgaaga ggacggcgac
     2101 cgccggcagg cgcgaggcga ggcgttcggc gcggccgccc cagaattccg ccgcgctcgc
     2161 ggcggtatag atcgcgaaca ccgccgacat catggcggtg cgcgaaaccg tcgacgcgta
     2221 gaagggctga tagccgcagg cggcgagcca gagcagcgcc cctgcggcga tcaagccaag
     2281 cggggtcctt ttgcccgcga aatcgcgcgc cgccgcgaac atgatcccat aggccagggc
     2341 catcagcccg gtcgcgatgt tgatcgagat gaagtcgggg agaacgccgc ggccgaacca
     2401 caggccggcg gcgattccgg ccaggatgtc gccgccgccc cacagcgcga gatagaaatt
     2461 acgacgacct tgcgtccagg agaaaaggat cagcccgccg agagtcgccg cgatgatggc
     2521 gatgagcgca agcgccgtgg gagcgtcaag ggatattgcc gacatggttc ttggggctcc
     2581 cgctcgccgc ctttttcccc gcgaatgtcg cgaaacctta tgggcgattg gtaaagcggc
     2641 ggcgcctttc gccgaccgtg tttaacgaag gcttggcgcg atccgaaaag atgccgaaaa
     2701 gcggctgacc gcggtccggc tttgccgaag caggttaggc gcgggacggc ggtcccgagg
     2761 ctgcgccggc taaaaatcgc aggcgccgct taaccacttt ctagccatgc gcccgtaagg
     2821 ctacattgcg gtgattcgcg tcgccttggc cgaggcggcc gaaaagcttg gcgtgcgggt
     2881 ccagcgtctt tttttcaatc atgcgccgaa gaaaatcatt gggggccaga gattgcgcag
     2941 gacaacccgg ccgcaaagcg ggctggcgcg aggcgccacg gctgaggcgc ggcgtctgat
     3001 cggcgcgccg gcgggcgcgt tcctgctcct tgcgctcgcc acggcgctgt cgccgcgggc
     3061 cgccttagcc agccaatcct tcgcgccttt cctcgccgtc gagccgacgc gggacaatcc
     3121 cgccggcctc gccgtgctga tcgggctcgt ccttttcgcc accttcacct cgatcctgca
     3181 tctggtcggc cgccgtcaat gggcgctgcg cgaaaaggcg ctgaccgccg agctgtcgca
     3241 actccaggcg cggctcgacc gcgccaacct ttttctctcc tccgatcagc agatcgtcgt
     3301 cgcttggggc tcggcgaccg gcgagccgga tgtcgaaggc gaatattccc tcgtcaccga
     3361 cgcgccgctg ccgcgccgcg tgctcggctt cggcgcctgg ctcgcgccgg aaccggccca
     3421 gaaacttgac caggcggtcg agcgcttgcg ccagcgcggc gagggattct cgctcaccgt
     3481 cgccagcctt gccggccgcc atttcgacat cgaaggccgc gcgatcgccg gccgcgccgt
     3541 gatgcgcata cgcgacattt ccggcgaccg gctgcaactt gtccgcctgc gcgagaacca
     3601 caatcggctc aatgaggaga tcggcgcgtt gcgcgccatg ctcaacgccc tgccaagccc
     3661 cgcctggacg cgcgacgcgc atggccgcat cgcctgggtg aatgcggcct atgcgaacgc
     3721 cgtcgaggcc aaggacgcga tcgacgcggc ggcgcgaggg ctcgaactgt tcgaccccgc
     3781 gacgcgcgag gcggcgctct ccgccctgca agccggcgaa acctggcggc ggcgcgcgcc
     3841 ggcggtcgca gccggcgagc ggcgcctcta cgatgtcgtc gaagtgccca ccggcgccgg
     3901 cgccgccggc atcgccaacg atctttccaa catcgtcgcg ctgcagggcg agctcgaaag
     3961 tcagatcgct tcgcacgctc gaacgctcga tcaattgtcg accggggtcg cgattttcga
     4021 ccgcgccaag cgcctcgtct tccataacgc cgcctatcgg cagatttggg cgctcgacgc
     4081 cgtgtttctc gagcagcagc cgaccgacga cgaaatcctc gaccggctgc gcgccaagcg
     4141 gcaattgccg gaacaggccg acttccgctc ctggaaagcg cagctcctcg ccgcctatca
     4201 ggcgatcgat ccggtcgagc ag
//
LOCUS       DATGJW010000288         5258 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_55687, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000288 DATGJW010000000
VERSION     DATGJW010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5258)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5258)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5258
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_55687"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(203..1234)
                     /locus_tag="VKU03_08835"
     CDS             complement(203..1234)
                     /locus_tag="VKU03_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71409.1"
                     /translation="MPREGSAVAGLGPATMAQRVASPEGATRREGSAFAGLGAVTMKE
                     LADNLSSARMRLLELLVFVAGIGAVYATIGGVRATISQDPFVFLRLFTTAHQPLPSFV
                     AFLGFLIPIVAIALGFDSISSEFNRRTMSRILAQPIYRDALLFGKFLAGLTTLAVMLV
                     ALWLLLTGLGILLLGLPPSGEEVMRGLVFLFAALAYGGVWLALAMLLSILFRSAATAA
                     LACLAIWLLFAFFWQMIVPIVTLWIAPVNPFDPFSQLHAINVGMDISRLSPNRLFAES
                     TLAILDPSTKALGPVLSSDVEGMITGSPLPFGQSMLLIWPQLTGLLAAMVLLFTLAYI
                     SFQRQEVRA"
     gene            complement(1224..2159)
                     /locus_tag="VKU03_08840"
     CDS             complement(1224..2159)
                     /locus_tag="VKU03_08840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009452091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71410.1"
                     /translation="MSEPIVRAQALTKRYGSTLAVDRLDLAIEEGEVFGLLGPNGAGK
                     TTTILMLLGLTEPTSGFARVAGFDPLRQPLEVKRRAGYMPDQLGFYDDLSGAANLRYI
                     ARLVGLPGREADARIAKALERVRLSEAAEKRVKTYSRGMRQRLAVAEILMKRASLAIL
                     DEPTSGLDPQSTREFLDLIRSLKADGVTIMLSSHLLDQVQSICDRVALFSRGKIGLMG
                     RVSDLLSEVLGGSHVIRVEAEGARLDEAVAKVPGVSRVAHEDGQLVVEAKGDLRAAIA
                     RAVVEAGGDLTMIAAGHASLADVYARYFEEVRDAA"
     gene            complement(2156..3328)
                     /locus_tag="VKU03_08845"
     CDS             complement(2156..3328)
                     /locus_tag="VKU03_08845"
                     /inference="COORDINATES: protein motif:HMM:NF022100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NEW3 domain-containing protein"
                     /protein_id="HLJ71411.1"
                     /translation="MLRRAAGLASVFLISATVSAFALDANAPPPIKGLWLATDFPSLT
                     LHAGGDTTLPLTLYSYGLAPQRTGLTIEGAPSGWKTEIDGAGKPVKAAFVDYDGKANL
                     SLKLTIPASAKPGPYNITIKAAGEDASSDLPIAIDLAAPLSAKLTATPQLPTLKGSPH
                     SSFDFKVSVKNDSPNDMLVNLTAGTPPGFETTFKEGYGSQELTSLPFKAGESKDVTVS
                     IKPSPDVKAGQYPIPVDFVGDKAKTETKLTLDIAGQPELSLTGQNDLLSGAAYAGDQT
                     NFPLVLRNTGSAPARDIKVSASEPSGWKISFEPKEIREIPVNGEQKVTAQVTPPAKAI
                     DGDYMISMTASGGGLSQSVNYRVTVLTSTMWGVAGIGVIGVALLVLVGAVRRFGRR"
     gene            complement(3501..4712)
                     /locus_tag="VKU03_08850"
     CDS             complement(3501..4712)
                     /locus_tag="VKU03_08850"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013253.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-hydroxy acid oxidase"
                     /protein_id="HLJ71412.1"
                     /translation="MNVANAVNIEDLRRLAQRRLPRILFDWIDGGAGDETALRQSMDG
                     FAATRFVPRYLAHISSCDLQTNLFGRAYALPFGLAPVGYVGLFWSGGELALAEAARRN
                     NTPFVLSNSAVATIEEIAKVAPEHTWFQLYPARDAGVTRDLIARAAAASFEGLVLTVD
                     LPVQAKRERDIRNKFTLPVRYSARNVLDGALHPAWSLQWLKNGGLPKMGSWAAYAGKD
                     ASAEKVAEYMRSQQHTPQSWADLEAYRAAWKGKLIVKGLQHPDDARKALAAGCDGIII
                     SNHGGRQFDRGPTPLESLPAVRQAVGDRIAVMIDGGVRRGADVLAALAHGADFVFCGR
                     AVVYGVAAAGLPGALRGFDILKDELRRTLGQIGLTRIADVGSEILWRPPPRHAEERSD
                     ADETIHSPGGA"
     gene            complement(4901..>5258)
                     /locus_tag="VKU03_08855"
     CDS             complement(4901..>5258)
                     /locus_tag="VKU03_08855"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HLJ71413.1"
                     /translation="LGELAHALAAAYGCVAEVEYERRYPPLITHAEETATSIGAAAAL
                     VGQENVIADAPPMTSAEDFSYMLQERPGGFMLIGNGVGPDGSFHDVHTPLYDFNDDIL
                     TLGAAYWVSLVQKELA"
BASE COUNT          938 a         1676 c         1818 g          826 t
ORIGIN      
        1 atgcaggttc tttcggaggt gagggtcagg cggggcgcaa aaatgacgat gccgaaagga
       61 aaaggcaagc ggcgccgcaa cgcgccacgt cattgcgggg agccgtaggg cgcgcttcgc
      121 gcgcccgtcc tacttataaa aacgcccggc tgaaagccgg gctatggcga cgaagcagtc
      181 cattcatggg gcggggcgtc ccctacgccc gcacttcctg gcgctggaag gagatatagg
      241 ccagcgtgaa gagcagcacc atcgcggcga gcaggccggt caattgcggc cagatcagca
      301 gcatgctttg gccgaaaggc aaaggcgagc cggtgatcat gccctcgacg tcgctggaca
      361 gtacggggcc gagcgccttg gtcgacggat cgagaatcgc caaagtcgat tcggcgaaaa
      421 gccggttggg cgacaggcgg ctgatgtcca tgccgacatt gatcgcatgg agctgcgaga
      481 acgggtcgaa aggattgacc ggggcgatcc agagcgtgac gatcggcacg atcatctgcc
      541 agaagaaggc gaagagcaac cagatcgcca ggcaggcgag cgccgccgtc gccgccgagc
      601 ggaagaggat cgacagcagc atggcgaggg cgagccagac gccgccatag gcgagcgcgg
      661 cgaagaggaa gaccaggccg cgcatgactt cctcgccgct cggcggcagg ccgagaagca
      721 ggatgccgag gccggtgagc agcaaccaga gcgcgacgag catcaccgcc agcgtggtca
      781 ggccggcgag gaatttgccg aacagaagcg cgtcgcgata gatcggctgc gccagaatgc
      841 ggctcatcgt gcggcgattg aactcgctgg agatcgaatc gaagccaagc gcgatcgcga
      901 cgatcggaat caagaagccc aggaaagcga caaaggacgg cagcggctga tgcgccgtgg
      961 tgaacaggcg cagaaacacg aacgggtcct ggctgatcgt cgcccgcacc ccgccgatcg
     1021 tcgcgtagac cgcgccgatg ccggcgacga agaccaggag ctccaacagc cgcatgcgcg
     1081 cactggagag attgtcggcg agctccttca tcgtcaccgc gccgagcccg gcgaaggcgg
     1141 agccttcgcg ccttgtcgcg ccctccggcg aggcgacgcg ctgcgccatt gtcgccggac
     1201 cgagcccagc gacggcggag ccttcacgcg gcatcgcgaa cctcctcgaa atagcgggcg
     1261 tagacgtcgg cgaggctggc gtgcccggcg gcgatcatgg tgaggtcgcc gccggcctcg
     1321 accaccgcgc gggcgatggc ggcgcgtaga tcgcctttcg cctcgacgac gagttggccg
     1381 tcctcgtgag cgacgcggga aacgccgggg actttggcga cggcctcgtc gagcctcgcc
     1441 ccttcggctt cgacccggat cacatgggag ccgccgagca cctcgctcaa aagatcgctg
     1501 acccggccca tcaacccgat cttgccgcgg ctgaacaaag cgacgcggtc gcagatcgac
     1561 tggacctgat cgagcaaatg cgaggacagc atgatcgtca cgccgtcggc tttcagcgag
     1621 cggatgaggt cgaggaattc gcgcgtcgat tgcggatcga ggccgctggt cggctcgtcg
     1681 agaatggcga ggctcgcccg cttcatcagg atttcggcga cggcgaggcg ctggcgcatg
     1741 ccgcgcgaat aggtcttgac ccgcttctcc gccgcctcgg agaggcggac gcgttcgagc
     1801 gctttggcga tgcgcgcatc agcctcgcgg cctggcaggc cgacgagccg ggcgatgtag
     1861 cgcaaattgg cggcgccgga gagatcgtcg tagaagccga gctgatcggg catatagccg
     1921 gcgcggcgct tgacttcgag cggctggcgc aagggatcga agccggcgac gcgggcgaag
     1981 cccgatgtcg gctcggtcag gccgagcagc atcaggatcg tcgtcgtctt gccggcgccg
     2041 ttcgggccga gcaggccgaa cacttcgccc tcttcgatcg ccagatcgag gcgatcgacc
     2101 gccagcgtcg atccgtagcg cttggtcagc gcttgcgcgc ggacgatcgg ctcgctcatc
     2161 gccggccgaa cctgcgcacc gcgccgacca ggaccagaag cgccacgccg atcacgccga
     2221 ttccggcgac gccccacatg gtcgaagtca ggacggtgac gcggtagttc accgattgcg
     2281 acaggccgcc gcccgacgcc gtcatcgaga tcatgtagtc gccgtcgatc gccttggccg
     2341 gcggcgttac ctgggcggtg accttctgct cgccattcac cgggatttcg cggatttcct
     2401 tcggctcgaa gctgatcttc cagccgctcg gctcggacgc gctgaccttg atgtcgcgcg
     2461 ccggcgcgct gccggtattg cgcaggacga gcgggaaatt ggtctgatcg cccgcatagg
     2521 cggcgccgct caggagatcg ttctggccgg tcagcgaaag ttcgggctgg ccggcaatgt
     2581 cgagcgtcag cttcgtctca gtcttggctt tgtcgccgac gaaatcgacc ggaatcggat
     2641 attgccccgc cttcacgtcg ggcgagggct tgatcgagac ggtcacgtcc ttgctctcgc
     2701 cggccttgaa cggcaggctg gtcaattcct gcgagccata gccttccttg aaggtcgtct
     2761 cgaagccggg cggcgttcca gccgtcaggt tgacgagcat gtcgttcggg ctgtcgttct
     2821 tgaccgacac tttgaagtcg aacgaagaat gcggcgagcc tttcaaggtc ggcaattgcg
     2881 gcgtcgcggt cagcttggcg gagagcggcg cggcgagatc gatcgcgatc ggcagatcgg
     2941 agctggcgtc ctcgccggcc gccttgatcg tgatgttgta agggccgggc ttcgccgaag
     3001 ccgggatcgt cagcttcagc gagagattcg ccttgccgtc atagtcgacg aacgcggctt
     3061 tgaccggctt gccggcgccg tcgatctcgg tcttccagcc ggagggcgcg ccctcgatgg
     3121 tcagaccggt gcgttgcggc gccaggccgt agctgtagag cgtcagcggc agcgtcgtgt
     3181 cgccgccggc atgcaaggtc aaggagggaa agtcggtcgc gagccacagg cccttgatcg
     3241 gcggcggcgc gttggcgtcg agagcgaagg cggaaacggt tgcggaaatg agaaagacgg
     3301 atgctaaccc ggccgcacgc ctgagcatgc caagacctcc cgatcgcatt gaaatgattg
     3361 caactcggca acgccgacgc gcgacagaaa ggccgcgact ttggcgaaaa ttggcaagaa
     3421 cagcggcaaa tttttgtaga tggtgcgaca gaatggctag gccgacgccg ccgcctgcga
     3481 gcgtcgcaaa gcgtccatcg tcaggcgccg cccggcgaat ggattgtttc gtccgcgtcg
     3541 ctgcgctcct cggcatgacg gggaggcggt cgccataaaa tctcgctgcc gacatccgcg
     3601 atgcgggtca ggccgatctg gccgagcgta cgccgcagct cgtccttcag gatgtcgaag
     3661 ccgcgcagcg cgccgggcag acccgcggcg gcgacgccat agacgacggc gcggccgcaa
     3721 aagacgaaat cggcgccgtg agccaaggcc gccagcacgt cggcgccgcg gcgcacgccg
     3781 ccgtcgatca tcaccgcgat tctgtcgccg accgcctgcc ggacggccgg cagcgattcg
     3841 agcggcgtgg ggccgcggtc gaattgccgg ccgccgtgat tggagatgat gatcccgtcg
     3901 cagccggccg ccagcgcttt gcgcgcgtcg tcgggatgct gcaaaccttt gacgatcagc
     3961 ttgcccttcc acgcggcgcg gtacgcctcg agatcggccc agctctgcgg cgtatgctgt
     4021 tgcgagcgca tatattcggc gaccttctcg gcgctggcgt ccttgccggc ataggcggcc
     4081 caggagccca ttttcggcaa gccgccattc ttcagccatt gcagcgacca ggccggatgc
     4141 agcgcgccgt cgagaacgtt gcgcgcgctg tagcgcaccg gcagcgtgaa cttgttgcgg
     4201 atgtcgcgct cgcgcttggc ctggaccggc agatcgacgg tgaggaccag cccctcgaac
     4261 gacgccgccg cggcccgcgc gatgaggtcg cgggtgacgc ccgcgtcgcg cgccgggtac
     4321 aactgaaacc aggtatgctc gggcgcgacc ttggcgatct cttcgatcgt cgcgaccgcg
     4381 ctgttggaaa ggacgaacgg cgtattgttc cgccgcgccg cctcggccaa agccaattct
     4441 ccgcccgacc agaacagccc gacatagccg accggcgcga gcccgaaggg caaggcatag
     4501 gcgcggccga aaaggttcgt ttgaaggtcg cagctcgaaa tatgagcaag atagcgcgga
     4561 acgaagcgcg tcgcagcgaa gccgtccatg ctctggcgca aagcggtctc gtcgcctgcc
     4621 ccgccgtcga tccagtcgaa gagaatgcgc ggcaagcggc gctgcgccag gcgccggagg
     4681 tcttcgatgt tgacggcgtt cgcgacgttc atgtctcgta ccttttttta tgtggcggcc
     4741 ttctcctccc ccgcgaagcg ggggaggggg accgcgcgtc agcgcggtgg agggggctcc
     4801 ggcccatcag gataatccca tccgccggcg gcccctccac cggccttcgg ccggtccccc
     4861 tcccccgttc cgcttcgctc cacgggggag gaagcgccgc tcaggccagc tccttttgca
     4921 cgaggctgac ccaataggcg gcgccgagcg tcaggatgtc gtcgttgaaa tcataaagcg
     4981 gcgtatggac gtcgtggaag gagccgtccg ggccgacgcc gttgccgatc agcatgaagc
     5041 cgccgggccg ctcttgcagc atgtaggaaa aatcctcggc gctggtcatg ggcggcgcgt
     5101 cggcgatcac gttttcctgc cctaccaaag cggcggccgc gccgatcgat gtcgccgtct
     5161 cctcggcgtg ggtgatcagg ggcggatagc ggcgctcgta ctccacttcg gcgacgcagc
     5221 cataggcggc ggcgagcgca tgggcgagct cgccgagg
//
LOCUS       DATGJW010000289         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_159964, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000289 DATGJW010000000
VERSION     DATGJW010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159964"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..468)
                     /locus_tag="VKU03_08860"
     CDS             complement(115..468)
                     /locus_tag="VKU03_08860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71414.1"
                     /translation="MPRNPAPPRTTALRRAALLGGLALAAALAATPAAAFVHMHGGGF
                     HGGSFHPHMAFNGFHHDFDRHFRHDFDRRFHRRFFGFAGFDFEPGYAYGYGAYDPCLR
                     RIWGPYGWRWVNVCY"
     gene            complement(607..903)
                     /locus_tag="VKU03_08865"
     CDS             complement(607..903)
                     /locus_tag="VKU03_08865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71415.1"
                     /translation="MLPIILIAAAISALPHFDIDRICRGDGSDRAKAEYKTCTGSEQT
                     ALAALQQKWAQYPAKLRKECAHVARVAPESTYVELQTCIESQIGDAKTGAAPGK"
     gene            1103..2245
                     /locus_tag="VKU03_08870"
     CDS             1103..2245
                     /locus_tag="VKU03_08870"
                     /EC_number="1.14.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF012946.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_function: GO:0050661 - NADP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLJ71416.1"
                     /translation="MDEPDAIVIGAGPAGLAAAQQLKAQGLRALIFDKAAAVGAVWRR
                     HYDRLHLHTPRRQSELPGLPMPAAYGRYPSRAQFVAYLERYAQAFALEPIFNAKVEAT
                     RRDGAVWRVAAGEHSARAPIVIVATGWADFPHAPDWPGMAEFRGPVIHSSVYRNAAPF
                     KDRRVLVIGFGNSGAEIALDLSEADAEAVLSVRSPVRVLPRDLLGAPILSFAIAQRFL
                     PAGLADALNAPILRLAIGSLDQFGLKTAAKGPLRMIKEDGRAPVIDIGALAQIRAGKI
                     KIRGGVERFTPDGVVFVEGGEERFDAVILATGFRPDLRTLLPDARGVLDEKGRPLVSD
                     RPTAAPGLFFVGAIVSPTGQLREIRLGAMRVAKAARRYLAEGSLVA"
     gene            2281..2385
                     /locus_tag="VKU03_08875"
                     /pseudo
     CDS             2281..2385
                     /locus_tag="VKU03_08875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007752652.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid maintenance system killer"
BASE COUNT          405 a          767 c          786 g          427 t
ORIGIN      
        1 cttagacgcg ccgtgcgaat ttggaagcca ttcggcagtc ggcaatcggc agatcggcag
       61 tctgtctagg cgaaatcccg actgccgact gccgactgcc gatctgccga cgtatcaata
      121 gcagacattc acccagcgcc agccgtaagg cccccagatc cgccgcaagc agggatcata
      181 agcgccgtag ccataggcgt atcctggctc aaaatcgaag ccggcaaagc cgaagaagcg
      241 gcgatgaaag cggcggtcga agtcatggcg gaaatgacga tcgaaatcgt gatgaaaccc
      301 gttgaaggcc atatgcgggt gaaagcttcc gccatgaaag ccgccgccat gcatgtgaac
      361 gaaggctgcg gcgggggtcg ccgcgagcgc ggcggcgagc gcgaggccgc cgagaagcgc
      421 ggcgcggcgc aaggcggttg ttcggggcgg cgcaggattt cgcggcatcg gtgcaactcc
      481 tctatgagcg cgagtcgcgc aaaagcgctc cgagcatgag cgccgccaca tgaacacgtc
      541 tcggctccaa actattttaa ggattggccg ctgggcggcg cgcctcacgg cgttgtgatg
      601 cgccctctac tttccgggcg ctgcgcccgt cttggcgtcg ccgatctgcg attcgatgca
      661 ggtctgcaat tcgacatagg tcgattccgg cgcgaccctc gccacatggg cgcattcctt
      721 tctgagcttg gccggatact gcgcccattt ctgctgaagc gcggccagcg ccgtctgctc
      781 agagccggtg caggtcttgt attccgcttt ggcccggtcg ctgccgtcgc cgcggcagat
      841 ccggtcgatg tcgaaatggg gaagggcgct gatggccgcc gcgatgagga tgatcggaag
      901 cacgggagga gctcctaacg ctagcgccgc taccgcttgc gccgcggctc cgcgccggtc
      961 gacaagctct cgtgaggaaa gcagaggatg gacaagcctt caagagagcc cggcgtctca
     1021 cgctttcgga tcaggcgaca ggaacgctcc gtcctgctat ttggggcaag cttgctcggg
     1081 gttgtcgtca agaggcgcta gcgtggatga gcccgatgcg atcgtgatcg gcgccgggcc
     1141 ggcgggactg gcggcggcgc agcaacttaa agcgcaaggc ttgcgcgccc tgattttcga
     1201 caaggccgcg gcggtcggcg cggtttggcg gcgccactac gatcgcctgc atctgcatac
     1261 gccgcgccgc caatccgaac tgcccggcct gccgatgccg gccgcttacg gccgctatcc
     1321 gtcgcgcgcg caattcgtcg cctatctcga gcgctacgcg caagcgttcg ctctcgagcc
     1381 gatcttcaac gctaaagtcg aggcgacccg ccgcgacggc gcggtttggc gcgtggcggc
     1441 gggcgaacat tcggcgcgcg cgcctatcgt catcgtcgcg accggctggg cggactttcc
     1501 tcatgcgccg gattggccag gcatggcgga gtttcgcggt ccggtgatcc attcgagcgt
     1561 ttatcgcaac gcagccccgt ttaaagatcg gcgcgttctc gtcatcggct tcggcaattc
     1621 cggcgccgag atcgcgctcg atctcagcga agccgacgcc gaggccgttc tcagcgtgcg
     1681 ctcgccggtg cgcgtcttgc cgcgcgatct gcttggcgcg ccgatcctca gcttcgcgat
     1741 cgcgcagaga ttcttgccag ccggcctcgc cgacgcgctc aacgcgccga tcctgcgcct
     1801 cgcgatcggc tcgctggacc agttcggctt gaaaaccgcg gcgaaggggc cgctgcgaat
     1861 gatcaaggag gacggccgcg cgccggtgat cgatatcggc gcgctcgctc aaatccgcgc
     1921 cggcaagatc aagatccgcg gcggcgtcga acggttcacg cccgacggcg tcgtcttcgt
     1981 cgaaggcggc gaggagcgct tcgacgccgt catcctcgcc accggcttca ggcccgattt
     2041 gcgaacgctt ctgcccgacg cgcgaggcgt gctcgacgaa aaagggcggc cgctcgtcag
     2101 cgaccggccg accgccgcgc ccggcctctt tttcgtcggc gcgattgtct cgccgaccgg
     2161 ccaattgcgc gaaatccgcc tcggcgctat gcgggtcgcc aaggcggcgc gaaggtattt
     2221 ggcggaagga tcgttggtcg cctgatgtca gcttatagtt gacgcttgtc gtcaattagc
     2281 ttccacgcgc tgcgcggttt cttgccgatt cgctacccgg tccatatgaa tgggccatgg
     2341 cgcatgactt tcgaattcga agacggcgac gccagcagga tcgac
//
LOCUS       DATGJW010000290        12457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_368121, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000290 DATGJW010000000
VERSION     DATGJW010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12457
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_368121"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2132)
                     /gene="purL"
                     /locus_tag="VKU03_08880"
     CDS             complement(<1..2132)
                     /gene="purL"
                     /locus_tag="VKU03_08880"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurL"
                     /protein_id="HLJ71417.1"
                     /translation="MSVTEPDITPALVAEHGLKPEEYQRFVKLIGRTPTFTELGIVSA
                     MWNEHCSYKSSRIHLRKLPTKAAWVIQGPGENAGVIDIGDGEACVFKMESHNHPSFIE
                     PYQGAATGVGGILRDVFTMGARPVACLNFLRFGSPDHPKTRHLLAGVVAGIGGYGNSF
                     GVPTVGGSLGFHKRYDGNILVNAMAVGIAKADEIFTAKATGVGNPIVYLGSKTGRDGI
                     HGATMASASFEADAEEKRPTVQVGDPFAEKLLLEACLELMKSGAVIAIQDMGAAGLTS
                     SAVEMGAKGDLGVELDLDRVPCREAGMTAYEMMLSESQERMLMVLHPEKEAEAEAIFR
                     KWGLDFAIVGQTTDTLRFVVKHRGEVKADLPIKELGDQAPVYDRPWIATPPRPVLKPS
                     DVTAPMSHREALLKLIGSPDFCSKRWVYEQYDTLILGNTVQPPGGDAAIIRLGDGPKG
                     LALTTDATPRYCEADPVAGGAQAVAEAWRNLIAVGARPLALTDNLNFGNPEKPEAMGQ
                     LVGCLLGIGEAATALDFPIVSGNVSLYNETSGVGILPTPAIGGVGLVEDIQKTASLAF
                     KAAGDIVLLIGETKGWLGRSAYLETICGREEGAPPPVDLAAEKRNGEFVRSLIQTGRV
                     SAVHDLSDGGLAVALAEMAIAGKIGATIDLGAAGLPAHAFLFGEDQGRYVVAARPTEA
                     AMIALEGKAIGLAVQTLGSTGGDALSLQG"
     gene            complement(2419..3639)
                     /gene="trpB"
                     /locus_tag="VKU03_08885"
     CDS             complement(2419..3639)
                     /gene="trpB"
                     /locus_tag="VKU03_08885"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit beta"
                     /protein_id="HLJ71418.1"
                     /translation="MSKVEKKNSFRNGPDDRGHFGIFGGRFVAETLMPLVLSLEKAYD
                     QAKADPAFKAELNHLLKHYVGRPSPLYFAERLTEHFGGAKIYFKREELNHTGAHKINN
                     VLGQILLARRMGKKRIIAETGAGQHGVATATACARFGLECVVYMGAVDIERQKPNVFR
                     MKMLGAEVRPVQSGSRTLKDAMNEALRDWVTNVENTFYCIGTVAGPHPYPAMVRDFQC
                     VIGEETRAQMMEAEGRLPDSLVACIGGGSNAIGLFHPFLDDPSVEIFGVEAAGHGLNV
                     PNGHAASLAGGRPGVLHGNRTYLLMDDDGQILEGHSISAGLDYPGIGPEHAWLKESGR
                     VTYLSATDKETLEAFQLCSKMEGIIPALEPSHALAKVAELAPKRAKDHLMVVGLSGRG
                     DKDIFSVAEHLGGM"
     gene            complement(3636..4334)
                     /locus_tag="VKU03_08890"
     CDS             complement(3636..4334)
                     /locus_tag="VKU03_08890"
                     /EC_number="5.3.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004640 - phosphoribosylanthranilate
                     isomerase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylanthranilate isomerase"
                     /protein_id="HLJ71419.1"
                     /translation="MLGTVKICGLSTAASLEAALAAGADMVGLVFFPKSPRHVSLAEA
                     RALAGQARGRAKIVALTVDADDRLLEEIVAAATPDLLQLHGRETPARVAAIAERFGAA
                     TIKAIGVSSAGDLAAAKPYAGAAAMMLFDAKPPKDANLPGGNGLAFDWRLLAGFANEL
                     PYLLSGGLTPDNVGEAIALSGAKGVDVSSGVESAPGVKDNDKIAAFVARARAAFAAQE
                     TKSAASERLAGRVA"
     gene            complement(4400..5194)
                     /locus_tag="VKU03_08895"
     CDS             complement(4400..5194)
                     /locus_tag="VKU03_08895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLJ71420.1"
                     /translation="MAEDKSSATLETTSDSWLGGKLMLRQPAKGHRVGSDAALLAAAA
                     PQADRIADIGAGIGAVGLALLSRMPKARADLVEIDAELAELARENAAANGFVERVRVA
                     VADVTSAPFRRGAGLVDGAADLVVTNPPFFEAGRAQASPEARRARAHILKNAGGGEGA
                     GLAAWLLGALALLAPGGCFVMIHRPEALGAILAAFENRLGAVALLPVHPRADAPAHRL
                     LIAGVKGARAPLSLRPGLVLHEPSGAFTETAEKLHCGEAILPLLAR"
     gene            complement(5187..5411)
                     /locus_tag="VKU03_08900"
     CDS             complement(5187..5411)
                     /locus_tag="VKU03_08900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2007 domain-containing protein"
                     /protein_id="HLJ71421.1"
                     /translation="MIELVRTNDLVMISVIEGLLTQERVAFFIADQHMSATEGSVGFL
                     PRRIMVDAEEAGRARRLLTAAGLAAELRDG"
     gene            5577..6590
                     /locus_tag="VKU03_08905"
     CDS             5577..6590
                     /locus_tag="VKU03_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HLJ71422.1"
                     /translation="MGIVIPLEEKAPERIIDRLTALVANDMALVNRTILSRTGSEVTM
                     IPEVANHLISSGGKRLRPMLTLAAAGLCGYAGDGHIKLAAAIEFMHTATLLHDDVVDE
                     SDMRRGKLAARMLWGNEASVLVGDFLLGQAFKMMVEVGSLPCLDVLSSAAAVIAEGEV
                     MQLSAAKDTATTEDDYLAVIRAKTAALFAAACEVGPILAGRSKAEVAACRGFGVNLGL
                     AFQLIDDALDYGGSSAKLGKNVGDDFREGKITLPVVLSFRRGTPDERAFWRRALEKGE
                     IADGDLETALAMLKKHRALEDTIERARHYGAMARDALELFPSSAWKHALLEAVEFAIE
                     RAH"
     gene            complement(6631..7524)
                     /locus_tag="VKU03_08910"
     CDS             complement(6631..7524)
                     /locus_tag="VKU03_08910"
                     /EC_number="2.7.1.148"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050515 - 4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-(cytidine
                     5'-diphospho)-2-C-methyl-D-erythritol kinase"
                     /protein_id="HLJ71423.1"
                     /translation="MTSPFAEPICGRVWRAPAKVNLTLHILGRREDGFHDLDSLVAFA
                     GIGDLLAFEPGPKLALSVDGPGAPTIGAAEDNLVLRAARLLQERVSGLRVGRFHLRKR
                     LPVAAGLGGGSSDAAAALRAVGHANALELDDPRLGEAARACGADVLVCLDPQARVMAG
                     IGDRVGPPLATPPLAAVLVNPGVAIATPAVFAKLGLAKGERADAGPSPDLRAAATPDD
                     IIAALQFGRNDMEAAARAIAPVVGDVLAALAESGAALARMSGSGATCFGLYRDRAASL
                     AAARALARRRPEWWVKATVLR"
     gene            complement(7642..9405)
                     /locus_tag="VKU03_08915"
     CDS             complement(7642..9405)
                     /locus_tag="VKU03_08915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383864.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ71424.1"
                     /translation="MLFTNRATLAARAARGASLGALLIALAGPALARDSVGAPSPLEI
                     GDTQSGNYLAALIAGDERDTSAAATYFREALRADPRNPDLIERAFAAALANGNMSDAF
                     DLADRLLVRDPNNSLARLTEACRAFAEGQYAVARAQLAGGGAGKAHDLTTSLLTAWTY
                     AGAGDSRRALDTLDRISDASVAVFRDYHAGLIASLLGNAPEAAKRLKAAYDAEKNTLR
                     LADAYARNLDRHGDAAGAKKIYEDMQRVLPHHPLVDSALADLAAGKTLDPIVRNAKDG
                     AAEVLYGLGAAGSRQGDELAALIYLRLALLLKPDHALAIVTVADLYLQLKQNQAAIDA
                     YEKVPRDSPMRVGADIQAALALDGMGRNGDAIKRLQDLVAAHPDNVEAISALGSLQRA
                     AKKFDVAADTYSKAIALLNPPERSNWTLFYFRGICYERSKQWPKAEADFKKALELFPD
                     QPLVLNYLGYSWVDQGVNLDEAFKMLQRAVDLRPSDGYIVDSLGWAYYKLGHYDQAVT
                     LLEKAVELKPADPVVNDHLGDAYWKVGRKLDAHFQWNHSRDMGPDPEDLPRILKKIQS
                     GLPDAPETPTAAQAEPKKDGG"
     gene            complement(9543..11219)
                     /locus_tag="VKU03_08920"
     CDS             complement(9543..11219)
                     /locus_tag="VKU03_08920"
                     /EC_number="1.5.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004174 -
                     electron-transferring-flavoprotein dehydrogenase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein-ubiquinone
                     oxidoreductase"
                     /protein_id="HLJ71425.1"
                     /translation="MAEDSALPPRESMDFDVVIVGAGPAGLAAAIRLKQLDSDRSVVV
                     VEKGSEVGAHILSGAVIDPIGLDRLLPDWRSDPDRPLKTQVTKDRFLVLGPSGGLTLP
                     NWPMPPLMSNHGNFIGSLGATTKWLAAQAEALGVEIYPGFAAAEILYGSKGEVLGVAT
                     GDMGVGRDGKPKAGFTRGMELRGKYTFIAEGARGSLAKELLARYGLEEGREPQKFGIG
                     LKEVWSVDPAKFQRGLVQHSFGWPLGRGASGGSFLYHYDDNLVSVGFVVHLNYVNPTL
                     SPFDEFQRFKTHPLIAGTFLGGKRLAYGARAISSGGWQSAPKLVFPGGALIGCAAGFV
                     NFARIKGTHNAILSGMLAAETAHEALSAGRAHDELTAYEESWRAGPIGRDLYKVRNVK
                     PLWTKLGLALGVALAGFDMWTNDIFGVSLFGTLKHGKPDYATLKPLAQVTPIAYPKPD
                     GRVSFDKLSSVFLSNTNHEEDQPVHLRLKDPTVPIRLNLPEYGEPARLYCPAGVYEVV
                     YGDEKTRSDPRFVINAQNCVHCKTCDIKDPAQNIVWTVPEGGGGPNYPDM"
     gene            11361..12194
                     /locus_tag="VKU03_08925"
     CDS             11361..12194
                     /locus_tag="VKU03_08925"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HLJ71426.1"
                     /translation="MTLTRPQDPIDRFGYSPEQILRWWAEAGVDAVLDEEPHNRFAEA
                     ATAAPAAPVPPPSAPRIAPRIEQAAAAPPDEAARSAREIAAQARSLDELKELLETFEG
                     CGLKATATQLAFADGSPDARVMLVGEAPGGEEDKAGKPFVGRAGQLLDRMLAAIGLDR
                     GKVYIANVVPWRPPGNRTPTLQETAICLPFIERQIELVGPDLLVCLGASSAQTLLGVK
                     DGITRARGKWREYRCGDKIIRALPMLHPAYLLRQPAQKRAAWRDLRALAGALAEIEKS
                     E"
     gene            12264..>12457
                     /locus_tag="VKU03_08930"
     CDS             12264..>12457
                     /locus_tag="VKU03_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71427.1"
                     /translation="MPSPSTRRAATPARLELRLIGTSDLHANIFAYDYYRDRPDDAVG
                     LAKTAALIRAARREKDNVLL"
BASE COUNT         2114 a         4098 c         4197 g         2048 t
ORIGIN      
        1 ccctgcaagc tcagcgcgtc gccgccggtc gagccgagag tctgcacggc aaggccgatc
       61 gccttgcctt cgagcgcgat catcgccgcc tccgtcggcc gcgccgcgac cacatagcgg
      121 ccctgatcct cgccgaacag gaaggcgtgc gccggcaaac cggcggcgcc gagatcgatc
      181 gtcgcgccga tcttgccggc gatcgccatt tcggccaacg ccacagcgag gccgccgtcg
      241 gaaagatcat ggaccgcgct gacccgaccg gtttggatca acgagcggac gaattcgccg
      301 ttgcgtttct cggcggcaag atcgaccggc ggcggcgcgc cttcctcgcg gccgcaaatc
      361 gtctccagat aggccgagcg gccgagccag cccttggttt cgccgatcag cagaacgatg
      421 tcgccggccg ccttgaacgc gagcgaggcg gttttctgaa tatcctcgac gaggccgacg
      481 ccgccgatcg ccggggtcgg cagaatgccg acgcccgatg tctcgttgta gagcgacaca
      541 ttgccggaga cgatcgggaa atcgagcgcc gtagccgctt cgccgatgcc cagaaggcag
      601 ccgaccaact gacccatcgc ctccggcttc tccggattgc cgaaattgag attgtcggtc
      661 aaagcgagcg gccgcgcgcc gaccgcgatc aggttgcgcc aggcctcggc gacggcttgc
      721 gcgccgcccg cgacgggatc ggcctcgcaa taacgcggcg tcgcgtccgt cgtcagcgcc
      781 aaccccttcg gcccgtcgcc gagccggatg atggcggcgt cgccgcccgg cggctgcacc
      841 gtattgccga ggatcagcgt atcgtactgc tcatagaccc agcgcttcga gcagaaatcc
      901 ggcgagccga tcagcttgag cagcgcctcg cgatggctca tcggcgccgt cacgtcgctc
      961 ggcttgagaa cgggccgcgg cggcgtcgcg atccagggcc ggtcatagac cggcgcctga
     1021 tcgccgagct ccttgatcgg caggtcggct ttgacctcgc cccgatgctt gacgacgaag
     1081 cgcagcgtgt cggtcgtttg gccgacaatc gcgaaatcga gcccccattt gcgaaaaatc
     1141 gcctcggcct cggcctcctt ttccggatgg agcaccatca acatgcgctc ctggctctcc
     1201 gacaacatca tctcgtacgc ggtcatgccc gcttcgcggc aaggtacgcg atcgagatcg
     1261 agctcgacgc cgagatcgcc tttggcgccc atttcgacgg ccgaggaggt caggcccgcc
     1321 gcccccatat cctggatggc gatcaccgcg ccggatttca tcaattcgag gcaggcttcg
     1381 agcagcagct tctcagcgaa cggatcgccg acctgcaccg tcggcctttt ttcctcggcg
     1441 tcggcctcga agctcgccga ggccatggtc gcgccgtgaa tgccgtcgcg gccggtcttg
     1501 gagccgagat agacgatcgg gtttccgacg cccgtcgcct tggcggtgaa aatctcgtcc
     1561 gccttggcga ttccgaccgc catggcgttg acgagaatgt tgccgtcata gcgcttgtgg
     1621 aagccgagcg agccgccgac cgtcggcacg ccgaacgaat tgccgtagcc gccgatgccg
     1681 gcgacgacgc cggcgaggag atggcgggtt ttcggatgat cgggcgagcc gaagcggagg
     1741 aaatttaagc acgcaaccgg ccgcgcgccc atggtgaaga cgtcgcgcag aatgccgccg
     1801 accccggtcg ccgcgccctg atacggctca atgaaggacg gatgattgtg gctctccatc
     1861 ttgaagacgc aggcctcgcc gtcgccaata tcgatcacgc cggcgttctc gcccgggcct
     1921 tggatgaccc aggcggcctt ggtcggcagc ttgcgcagat ggattcgcga agatttatac
     1981 gagcaatgct cgttccacat cgccgagacg atgccgagct cggtgaaagt cggcgtccgg
     2041 ccgatcagct tgacgaagcg ctggtattct tccggtttca ggccgtgctc ggcgaccagg
     2101 gccggcgtga tgtcaggttc cgtgacggac aaggatggcc tcaggggcgg ctggtcaggc
     2161 ccgcttagac aatggcggcg cccgccgcaa ggggcgcgct ttgccgtcgt ctcgttggaa
     2221 gaaagccgcc gcgctccctc gacagcctcg gccgacttgc ccggccatag ccgcaacttc
     2281 ggccgttgga gcaagattcg ccctctttgg acgcagaaaa gctctaatct atattagcta
     2341 gaatcagcta aaggcgagct catgaccgta gtcaacatgc tcgaagctta aagataagcg
     2401 cagaggcgac acgcctcctc acatcccgcc gagatgctcc gcgacgctga aaatgtcctt
     2461 gtcgccccga cctgacaggc cgacgaccat gaggtgatcc ttggcccgct tcggcgcgag
     2521 ctccgccact ttcgccaagg cgtgcgacgg ctccaaagcg gggatgatgc cctccatttt
     2581 cgagcagagc tgaaaggcct ccagcgtctc cttgtcggtc gccgagaggt acgtcacccg
     2641 gccgctctcc ttcagccaag cgtgctccgg gccgatgccg ggatagtcga gcccggcgga
     2701 gatcgagtga ccttcgagga tctggccgtc atcgtccatc aggaggtagg tgcggttgcc
     2761 gtgcagcacg ccgggccggc cgccggccag cgaggcggcg tggccattcg ggacgttgag
     2821 cccgtggccc gcggcctcga cgccgaaaat ctcgacggaa ggatcgtcga ggaaaggatg
     2881 gaacagcccg atcgcgttgg agccgccgcc gatgcaggcg acgaggctgt ccggcaggcg
     2941 gccctcggcc tccatcatct gggcgcgcgt ctcctcgccg atcacgcatt gaaaatcgcg
     3001 caccatcgca gggtagggat gcggcccggc gacggtgccg atgcaataga acgtattttc
     3061 gacattggtg acccagtcgc gcagcgcctc gttcatcgcg tctttcagcg tgcgcgagcc
     3121 ggattggacc ggccgcactt cggcgccgag cattttcatc cggaagacgt tcggcttctg
     3181 ccgttcgata tcgaccgcgc ccatatagac gacacattcg aggccgaagc gcgcgcaggc
     3241 ggtggcggtg gcgacgccgt gctggccggc gcccgtctcc gcgatgatcc ttttcttgcc
     3301 catccggcgg gcgagcagaa tctggccgag cacgttgttg atcttgtgcg cgccggtatg
     3361 attgagttct tcgcgcttga aatagatttt cgctccgccg aagtgttcgg tgaggcgctc
     3421 ggcgaaatag agcggactcg gccggccaac atagtgcttg agcaaatggt tgagctcggc
     3481 cttgaaggcc ggatcggcct tggcctggtc ataggccttt tccaacgaaa gtacgagcgg
     3541 catcagcgtc tcggcgacga agcggccgcc gaatatgccg aaatggccgc gatcgtccgg
     3601 gccgttgcgg aaggaattct tcttttcgac cttgctcacg cgaccctccc ggccagcctt
     3661 tcgctcgccg ccgatttcgt ttcctgcgcc gcgaaagccg cgcgggcgcg cgcgacgaaa
     3721 gcggcgatct tgtcgttgtc cttgacgccc ggggcgcttt cgacgccgga cgaaacgtca
     3781 acgcccttgg cgccgctcag cgcgatcgcc tcgccgacat tgtcgggcgt cagcccgccg
     3841 gaaagcagat agggcagctc attggcgaag ccggcgagca ggcgccagtc gaaggccaag
     3901 ccgttgccgc cgggcaggtt tgcgtctttc ggcggcttgg cgtcgaacag catcatcgcg
     3961 gcggcgccgg cgtaaggctt cgccgcggcg agatcgccgg ccgaagagac gccgatcgcc
     4021 ttgatcgtcg cggcgccgaa cctttcggcg atcgccgcga cgcgcgccgg cgtctcgcgg
     4081 ccatgcaatt gcagcaaatc cggcgttgcc gcggcgacga tttcttcgag caggcgatcg
     4141 tcggcgtcga ccgtgagcgc gacgatcttg gcgcgcccgc gcgcctggcc ggcaagcgcg
     4201 cgcgcctcag cgagcgagac atggcgcggg cttttcggga aaaagacgag tccgaccata
     4261 tcggcgcccg cggcgagcgc cgcttcgagc gaggcggcgg tggaaaggcc gcagattttg
     4321 acggtcccaa gcatcaggct ccctttcggc caaaccgccg gggttgtcaa agggagacgg
     4381 ccgaagatcg agagaaggct caacgcgcaa ggagaggaag gatcgcctcg ccgcaatgca
     4441 gcttttccgc cgtctcggtg aatgcgcccg acggctcatg caggacgagg ccaggtctta
     4501 agctcaaagg cgcgcgggcg cctttgacgc cggcgatcag caggcgatgg gcgggcgcgt
     4561 cggcgcgcgg atggaccggc aaaagcgcga ccgcgccgag gcgattttcg aacgccgcca
     4621 gaattgcgcc caacgcctcc ggccgatgga tcatgacgaa gcatccgcct ggggcgagca
     4681 gcgcgagcgc gcccaaaagc caagccgcaa gcccggcgcc ttcgccgccg ccagcatttt
     4741 tcaagatatg cgcccgcgcc cgcctggcct ccggcgaggc ctgggctctt cccgcctcga
     4801 agaaaggcgg gttcgtcacg acgagatcgg ccgcgccgtc gacgaggccg gcgccgcggc
     4861 ggaagggcgc gctggtcacg tcggcgacgg cgacgcggac gcgctcgaca aagccgttcg
     4921 ccgcggcgtt ctctcgcgcc agctcggcaa gctcggcgtc gatctcgacg aggtcggcgc
     4981 gcgccttcgg catacggctg agcaaagcca acccgaccgc gccgattcct gccccgatat
     5041 cggcgatgcg atcggcttgc ggcgcggcgg cggcgagcaa agcggcgtcg ctgccgacgc
     5101 gatggccctt ggccggttgg cgcagcatca gcttgccgcc gagccaggaa tccgaggtgg
     5161 tttcgagagt ggccgaggat ttgtcctcag ccatcgcgca attcggcggc gaggccggcg
     5221 gccgtcagca gccggcgcgc gcgccccgcc tcctcggcgt cgaccatgat ccggcgcggc
     5281 aggaagccga ccgagccttc ggtcgcgctc atatgctgat cggcgatgaa aaaggcgacg
     5341 cgctcctgcg tcaaaaggcc ctcgatgacg gagatcatga ccagatcgtt ggtgcgaacg
     5401 agttcgatca cattggcccc atggctcgaa agcggcggcg cttcgccgat ctctaagatt
     5461 atgcgagcct cgccgctccg ggcaatcggc cggaatcaca gtttcgggac gcgacttgcc
     5521 cggccggaag gccgtcttta aggtcgagac ggtttaggcg acaagggaaa gaccccgtgg
     5581 gaattgtcat tccgctcgag gaaaaggcgc cggagcgcat catcgaccgg ctcaccgccc
     5641 tggtcgccaa cgacatggcc ttggtcaacc ggacgatttt gtcgcggacc ggctccgagg
     5701 tcacgatgat cccggaagtc gccaatcacc tgatctcgtc gggcggcaaa aggctgcggc
     5761 cgatgctgac cctcgccgcg gccgggctct gcggctatgc cggcgacggc catatcaagc
     5821 tcgccgcggc gatcgaattc atgcatacgg cgaccttgct gcatgacgac gtggtcgacg
     5881 aaagcgacat gcgccgcggc aagctcgccg cccgcatgct ctggggcaat gaggcgagcg
     5941 tcctggtcgg cgatttcctg ctcggccaag ccttcaagat gatggtcgag gtcggctcgc
     6001 tgccctgtct cgacgtgctc tctagcgccg ccgcggtgat cgccgaaggc gaggtgatgc
     6061 agctttcggc ggccaaggac acggcgacga ccgaggatga ttatctcgcc gtgatccgcg
     6121 ccaagacggc ggcgcttttc gccgcggcct gcgaggtcgg gccgattctc gccggccgct
     6181 ccaaggccga ggtcgccgcc tgccgcggct tcggcgtcaa tctcggcctg gcctttcagc
     6241 tcatcgacga cgcgctcgat tatggcggct cctccgccaa gctcggcaag aatgtcggcg
     6301 acgatttccg cgaggggaag atcactctgc ccgtcgtcct ctccttccgc cgcggcacgc
     6361 cggacgagcg cgccttctgg cggcgagctc tcgaaaaagg cgagatcgcc gacggcgatc
     6421 tcgagactgc gctcgccatg ctgaaaaagc accgcgccct cgaagacacg atcgagcgcg
     6481 cccgccatta cggcgccatg gcgcgcgatg cgttggagct gttcccctct tcggcctgga
     6541 agcacgcgct gttggaggcg gtggagttcg cgatcgagcg cgcgcattga gtttcctccc
     6601 cctgttccgc ttcgcggaac ggggggatag ctacctcagc acagtcgcct ttacccacca
     6661 ttccgggcgc ctccgcgcga gagcgcgcgc cgccgccaag ctcgcggcgc ggtcgcggta
     6721 caggccgaag caggtcgcgc ccgagcccga catgcgcgcc agcgccgcgc cgctttcagc
     6781 gagcgccgcg agaacgtcgc cgacgacggg cgcgatcgcg cgcgccgcgg cctccatatc
     6841 gttgcggccg aactgcagcg ccgcaatgat gtcgtccggc gtagcggcgg cgcgaagatc
     6901 aggcgacggc ccggcatcgg cgcgttcgcc cttggcgagg ccgagctttg cgaacacggc
     6961 gggggtcgcg atcgcgacgc ccggattgac cagcaccgcg gcaagcggcg gcgtcgcgag
     7021 cggcgggccg acccgatcgc cgatcccggc catgacccga gcttgcggat caagacagac
     7081 aagcacgtcc gccccgcagg cgcgcgccgc ctcgccgagg cggggatcgt caagttcgag
     7141 cgcgtttgcg tgccccacgg cgcgcaaggc ggcggcggcg tccgaggagc cgccgccgag
     7201 gccggcggcg acgggcagcc ttttgcgcaa atgaaagcgg ccgacgcgaa ggccggagac
     7261 gcgctcttgc agcaggcgcg cggctctaag gacgaggttg tcctcggccg cgccgatcgt
     7321 cggcgcgcca ggtccgtcaa cgctgagcgc gagcttcggc ccaggctcga aggcgaggag
     7381 atcgccaatt ccagcgaaag cgacgaggct gtcgagatcg tggaagccgt cctcgcgccg
     7441 gccgagaata tggagggtga gattgacctt ggccggcgcg cgccaaaccc ggccgcagat
     7501 cggctcggcg aaaggcgagg tcacggcgct ttgtcattgc gagcgaagcg aggcaatcct
     7561 ctcgtagagc gcagccctac gaatggattg cttcgtcgcg tcgctgcttg caatgacggc
     7621 gtcaccggcg gcgcgcagcg ctcagccgcc atccttcttc ggctcggcct gggcggccgt
     7681 cggcgtttcc ggcgcgtcgg gcaggccgga ttggatcttc ttcaagatgc gcggcaagtc
     7741 ttccggatcg ggacccatgt cgcgcgaatg gttccattgg aaatgcgcgt cgagcttgcg
     7801 gccgactttc caataggcgt cgccgagatg gtcgttgacg accggatcgg ccggcttcaa
     7861 ctcgaccgcc ttttcgagca gcgtcaccgc ctgatcgtaa tggccgagct tgtaataggc
     7921 ccagccgagg ctgtcgacga tatagccgtc ggacggccgc aaatcgacgg cgcgctgcag
     7981 catcttgaac gcttcgtcga gattgacgcc ctgatcgacc caggaatagc cgagatagtt
     8041 gagaacgagc ggctgatccg ggaagagctc cagcgccttc ttgaaatcgg cttccgcctt
     8101 cggccattgc ttcgagcgct cgtagcagat gccgcggaaa tagaacaagg tccagttgct
     8161 gcgctcgggc ggattgagca gcgcgatcgc cttggagtag gtgtccgccg cgacgtcgaa
     8221 tttcttggcg gcgcgttgca ggctgccgag cgcgctgatc gcctcgacat tgtcgggatg
     8281 cgcggcgacg agatcctgca ggcgcttgat cgcgtcgcca ttgcggccca tcccgtccag
     8341 ggcgagggcc gcctggatgt cggcgccgac gcgcatcggc gaatcgcgcg gcaccttctc
     8401 gtaggcgtcg atcgccgcct ggttctgctt gagctgcaga tagagatcgg cgaccgtgac
     8461 gatggccagc gcatggtcgg gcttcaagag caaagccagg cgcagataga tcagcgcggc
     8521 gagctcatcg ccttgccggc tgccggcggc gccgaggcca taaagcacct cggcggcgcc
     8581 gtccttggcg ttgcggacga tcgggtcgag cgtcttgccg gcggcgagat cggcgagcgc
     8641 gctgtcgacc aggggatgat gcggcaggac gcgctgcatg tcctcgtaga tttttttcgc
     8701 gccggcggcg tcgccatgcc ggtcgaggtt gcgcgcatag gcgtcggcga ggcgaagcgt
     8761 gttcttctcg gcgtcatagg cggctttgag ccgcttggcc gcttcgggcg cgttgccgag
     8821 caggctggcg atcagcccgg cgtgatagtc gcggaacacc gccaccgagg cgtcgctgat
     8881 ccgatcgagc gtatcgagcg cgcgacggga atcgccggcc ccggcatagg tccaagcggt
     8941 cagcaaagag gtggtcaggt catgcgcctt gccggcgccg ccgccggcca attgggcgcg
     9001 cgccacggca tattgtccct cggcgaaggc gcggcaggcc tcggtcaggc gagcgagcga
     9061 attgttcggg tcgcggacga gaagacgatc ggcgagatcg aacgcatcgg acatattgcc
     9121 attggcgagg gcggcggcga aggcgcgctc gatcagatcg gggttgcgcg gatcggcgcg
     9181 caaagcctcg cgaaagtacg tcgcggccgc gctggtgtcg cgttcgtcgc cggcgatcag
     9241 ggcggcgaga taattgcccg actgcgtatc gccgatttca agcggcgaag gcgcgccgac
     9301 gctgtcgcgg gcgagagcgg gccccgccaa ggcgatgagg agagcgccga gcgaggcgcc
     9361 acgtgcggcg cgagcggcga gggtggcgcg gttcgtgaaa agcacttgcc ctgatttcct
     9421 ttcccgccat ggcggcggag cgcggacgga acgatcgctg agatcccctt ataggccaat
     9481 ccctcgcggc gaaaaatggc ggttttacgc accttacggt tttccccgcg ctgttcgcag
     9541 cgtcacatat cgggataatt tggcccgccg ccgccttccg gaaccgtcca gacaatgttc
     9601 tgcgccgggt ccttgatgtc gcacgttttg cagtggacgc aattttgcgc gttgatgacg
     9661 aagcgcgggt cggagcgggt cttttcgtcg ccatagacca cttcataaac gccggccggg
     9721 caataaagcc gcgccggctc gccatattcg ggcaggttga gccggatcgg aacggtcgga
     9781 tccttgaggc gcagatgaac cggctggtcc tcttcatgat tcgtgttgga cagaaatacc
     9841 gacgagagct tgtcgaaaga gacgcggccg tcaggcttgg gataggcgat cggcgtcact
     9901 tgagcgagcg gcttcaaggt cgcgtaatcg ggcttgccgt gtttcagcgt gccgaacagc
     9961 gagacgccga aaatatcgtt ggtccacatg tcgaagccgg ccagcgcgac gcccagcgcg
    10021 agcccgagct tcgtccataa cggcttgacg ttgcgcacct tatagagatc gcgcccgatc
    10081 ggcccggcgc gccagctctc ctcataggcg gtcagttcgt catgcgcgcg gccggcgctc
    10141 agcgcctcat gcgcggtctc ggcggcgagc atgccggaaa ggatcgcgtt atgcgtgccc
    10201 ttgatccgcg cgaaattgac aaagccggcg gcgcagccga tcagcgcgcc gccggggaag
    10261 acgagtttcg gcgccgattg ccagccgccc gagctgatcg cccttgcgcc ataggcgagg
    10321 cgcttgccgc cgaggaatgt gccggcgatc agcggatggg tcttgaagcg ctggaattcg
    10381 tcgaaaggcg agagggtcgg attgacgtaa ttgagatgga cgacaaagcc gacggagacg
    10441 agattgtcgt cgtaatggta aaggaacgag ccgccgctcg cgccgcgccc gagcggccaa
    10501 ccgaacgagt gctggacgag gccacgctgg aacttggccg gatcgaccga ccagacttcc
    10561 ttgaggccga tgccgaattt ctgcggctcc cgcccctcct ccaacccgta gcgggcgagc
    10621 agctcctttg ccagcgagcc gcgcgcgcct tcggcgatga acgtatattt gccgcgcagc
    10681 tccatgccgc gggtgaagcc ggctttcggc ttgccgtcgc ggccgacgcc catatcgccg
    10741 gtggcgacgc cgaggacctc gcctttgctc ccgtaaagaa tttcggcggc ggcgaaaccc
    10801 ggatagattt caacgcccag agcctccgcc tgggcggcca gccatttggt cgtcgcgccg
    10861 agcgagccaa tgaaattgcc gtgattgctc atcagcggcg gcatcggcca attgggcagg
    10921 gtcaagccgc cggaggggcc gaggacgaga aagcggtctt tcgtgacctg cgtcttgagc
    10981 ggccggtccg gatcgctgcg ccagtcgggc agcaggcggt caagcccgat cgggtcgatc
    11041 accgcgccgg acaggatatg cgcgccgact tccgagccct tttcgaccac gacgaccgaa
    11101 cgatcggaat cgagttgctt gagccggatc gccgccgcaa ggccggccgg cccggcgccg
    11161 acgatgacca cgtcgaaatc catcgattcg cgcggcggca gggcggagtc ttcggccatc
    11221 tctcctccgg cgtcggccgc tcttggcgcg gccgttgcta ggcgataagt caataccgac
    11281 cgatcgccgt tttggaccgg ccgcaaagaa aagtcgagat tccgccggcg ccgcattccc
    11341 gatcgcaaag ctttctcgtc gtgaccctga ctcgcccgca agacccaatc gaccgcttcg
    11401 gctattcgcc ggagcagatt ctgcgctggt gggccgaggc gggggtcgac gccgtcctcg
    11461 acgaggagcc tcacaatcgg ttcgcggaag cggcgaccgc cgcgccggcc gcgccagttc
    11521 cgccgccctc cgccccccgc atcgcgccgc ggatcgagca ggcggcggcc gcgccgccgg
    11581 acgaggcggc gcgctcggcg cgcgaaatcg ccgcgcaggc ccgatcgctc gacgaactca
    11641 aggaattgct cgagacgttc gaaggctgcg gcctcaaggc gacggcgacc cagctcgcct
    11701 ttgccgacgg ctcgcccgac gcgcgggtga tgctggtcgg ggaagcgccg ggcggcgagg
    11761 aagacaaggc tggcaagccg tttgtcggcc gcgccgggca attgctcgac cggatgctgg
    11821 cggcgatcgg cctcgaccgc ggcaaggtct atatcgccaa cgtcgtgccc tggcggccgc
    11881 ccggcaaccg cacgccgacc ttgcaggaga cggcgatctg cctgcctttc atcgagcggc
    11941 agatcgaact ggtcggcccc gatctgctcg tctgtctcgg cgcctcctcc gcgcaaaccc
    12001 tgctcggcgt caaggacggc ataacccgcg cgcgcggcaa atggcgcgaa tatcgctgcg
    12061 gcgacaagat aatcagggcg ctgcccatgc tgcacccggc ctatctgctg cgccagccgg
    12121 cgcagaaacg ggcggcatgg cgggatttgc gggcgctggc gggcgcgctg gcggaaatcg
    12181 agaagagcga atagggaatg gcgaatagcg aatgggaggg gagctccatt cgccattcgc
    12241 cattcgctcc atcgcggagt cctatgccct cgccgtcgac ccgacgcgca gcgacgccgg
    12301 ccaggctaga gcttcgcctc atcggcacca gcgacctcca cgccaacatc ttcgcgtacg
    12361 actactatcg ggaccgtccg gacgacgcgg tcgggctcgc caagacggcg gccttgatcc
    12421 gcgccgcccg gcgcgagaag gacaacgttc ttctctt
//
LOCUS       DATGJW010000291         3737 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_160049, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000291 DATGJW010000000
VERSION     DATGJW010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3737)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3737)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3737
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160049"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..399
                     /gene="vsr"
                     /locus_tag="VKU03_08935"
     CDS             13..399
                     /gene="vsr"
                     /locus_tag="VKU03_08935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004520 - DNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch endonuclease Vsr"
                     /protein_id="HLJ71428.1"
                     /translation="MDRSAVMRAVKSQDTAPERALRKLLHAVAPGYRLHRADLPGKPD
                     IVYGPRRLAIFMHGCFWHGHDCKRGARPPKHNAEYWRGKIARNRARDEKNVAALEALG
                     WRTLTVFECELKDEGALMERVKRALL"
     gene            complement(498..1052)
                     /locus_tag="VKU03_08940"
     CDS             complement(498..1052)
                     /locus_tag="VKU03_08940"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018268306.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008752 - FMN reductase (NAD(P)H) activity
                     [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0052873 - FMN reductase (NADPH) activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent FMN reductase"
                     /protein_id="HLJ71429.1"
                     /translation="MKILAISGSLRAASINTAILQAAKRLAPSDAEIVLYRDLAGLPA
                     FNPDDDRDPPPAPAAALRAAVGVAEGLVFACPEYAHGLPGALKNALDWLVGSTEFPGK
                     PVMLINAAPRAFHAQASLREVLSTMSAQLVADAFVTISAPRDANAEALLADPAIAGPL
                     QAGLERFAAVLRAQARRTGEVFPV"
     gene            1222..1410
                     /gene="rpmF"
                     /locus_tag="VKU03_08945"
     CDS             1222..1410
                     /gene="rpmF"
                     /locus_tag="VKU03_08945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003601373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L32"
                     /protein_id="HLJ71430.1"
                     /translation="MAVPRRKTSPSRRDMRRSADALKAPTYVEDKDSGELRRPHHVDL
                     KSGMYRGRQILKPKKVEA"
     gene            1480..2226
                     /locus_tag="VKU03_08950"
     CDS             1480..2226
                     /locus_tag="VKU03_08950"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ71431.1"
                     /translation="MSKADRDLVRAIEERGFNSWPALRTALVDGWIVRLSDGHTRRAN
                     SASPLWPSRLPPKALIAAIEPLFRQAVLRPVFRITPLADPAVERHLVAAGWRDDDPSL
                     GMFAGDPLGAADPAVIFESQASEAWIAAAMEAYGHGEPGAAALRRLLPLIAVPAVFAT
                     LIEAGEPLAFGLAAAERGMVGFHDIVVAPKARGRGLGRRLVGALIDWGRRRGAPQAYL
                     LVREGNRPARALYRSLGFVEAYRYANMVRG"
     gene            2565..3143
                     /locus_tag="VKU03_08955"
     CDS             2565..3143
                     /locus_tag="VKU03_08955"
                     /inference="COORDINATES: protein motif:HMM:NF039656.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6101 family protein"
                     /protein_id="HLJ71432.1"
                     /translation="MELERPFAMSAERFAPKPGAAEARRIIAADPRADGRRREVMLAR
                     GRVQIARSVAGVRMKISLAASAYRGVILRLRGFEAQRFSYEIELAHRDPDLSVTLLEA
                     SDDGEVQAEWRLWARFLGLPALVEREEGRPEPDAARLGELAVSPRKPRRRGGAMARRR
                     PRFLTRRKTGRGSPGPSLAGAREIFPGSKDGR"
     gene            complement(3239..>3737)
                     /locus_tag="VKU03_08960"
     CDS             complement(3239..>3737)
                     /locus_tag="VKU03_08960"
                     /EC_number="3.2.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019691833.1"
                     /note="Catalyzes the hydrolysis of AMP to form adenine and
                     ribose 5-phosphate using water as the nucleophile; Derived
                     by automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AMP nucleosidase"
                     /protein_id="HLJ71433.1"
                     /translation="LAHAYLRQDHILDDAVPLDMPIPALAEVQVALQEAAAQVTGDRG
                     EALKRRLRTGTVVTNDDRNWELRWTQERRRINLSRAIAVDMESGTIATQGFRMRVPYG
                     ALLCVSDKPLHGEIKLPGAANAFYERAVGEHLQIGLAAVDMLRGQRATLHSRKLRSFD
                     EPPFR"
BASE COUNT          582 a         1252 c         1303 g          600 t
ORIGIN      
        1 ccccgcgtcg cgatggaccg ctccgcggtc atgcgggccg tcaaatcgca agacaccgcg
       61 ccggagcgcg ccttgcgcaa gctgctccac gccgtcgcgc cgggctatcg gctgcatcgc
      121 gccgatctgc cgggcaagcc ggatatcgtc tacggcccgc gccggctcgc cattttcatg
      181 catggctgct tctggcacgg ccatgattgc aagcgcggcg cccggccgcc gaaacacaac
      241 gccgaatatt ggcgcggcaa gatcgcccgc aaccgcgccc gcgacgagaa gaacgtcgcc
      301 gcgctggagg cgctcggctg gcgcacgctg acggttttcg aatgcgagct gaaggacgag
      361 ggcgcgctca tggagcgcgt gaagcgggct ctcctgtgat cggtttcaga agctgcggca
      421 gcgccgcgct gtagccgcgg tcgccgaccg cggctacaat atggcgccgc ggcgtttagt
      481 ctaacgtacg gacgcctcta aaccggaaaa acctcgcccg tgcgccgcgc ttgcgcgcga
      541 agcacagcag cgaagcgttc gagcccggct tgcagcggcc cggcgatcgc cggatcggcg
      601 agcagcgcct cggcgttcgc gtctctcggc gcgctgatcg taacgaaggc gtcggcgacg
      661 agctgggccg acatggtcga caaaacctcg cgcaaggacg cctgcgcatg gaaggcgcgc
      721 ggcgcggcgt tgatcagcat caccggcttg ccgggaaatt cggtcgagcc gaccagccag
      781 tcgagcgcgt tcttgagggc gccgggcagg ccatgcgcgt attcggggca tgcgaagacg
      841 agaccttcgg cgacgccgac cgccgcgcgc aaagccgcgg ccggggcggg cgggggatcg
      901 cggtcgtcgt cagggttgaa ggccggcaag ccggcaagat cgcggtaaag gacgatttcc
      961 gcgtcggacg gcgccagccg cttcgccgct tggaggatcg ccgtattgat cgaagccgcc
     1021 cgcaagctgc cggaaatcgc gagaatcttc atgggcctgt ccgaatcgag agcgctttgg
     1081 agcataagcg cgggggccgc gccagatcgc cggccttggc ggcaaaaggc tagccaggcc
     1141 cggtctttcc gtgtaagagc gccgctttcc ggcgggcttg cccggctctt ggccggaagt
     1201 gaagccccat tggagagttg aatggctgtt ccgagacgaa aaacgtcgcc ttcgcgacgc
     1261 gatatgcgcc gctcggccga cgcgctcaaa gccccgacct atgtcgagga caaggattcg
     1321 ggcgaattgc gccggcccca tcatgtcgac ctcaagagcg gcatgtatcg cgggcgccag
     1381 atcctcaagc ccaagaaggt cgaggcgtaa ggcttcgctt ttcagaaggc ttgagagaag
     1441 ggccggcctc gcgccggcct tttcattttc gaggggacga tgagcaaagc cgatcgcgat
     1501 cttgtccgcg ccatcgagga gcgcggcttc aattcctggc cggctctgcg gactgcgctc
     1561 gtcgacggct ggatcgttcg cctctccgat gggcatacgc gacgcgccaa ttcggcgagc
     1621 ccgctctggc cttcgcgcct cccgccgaag gcgctgatcg ccgcgatcga gcctttgttt
     1681 cgccaggcgg ttctgcgccc tgtcttccgg atcacgccgc ttgccgatcc cgccgtcgag
     1741 cgccatctcg ttgcggccgg ctggcgcgac gacgatcctt cgctcggcat gttcgccggc
     1801 gacccgctcg gcgccgccga tccggcggtg attttcgaat cgcaggcgag cgaagcctgg
     1861 atcgccgccg cgatggaagc ctatggccat ggcgagccgg gcgcggcggc tttgcgccgc
     1921 ctgctgccgc tgatcgccgt cccggccgtc ttcgccaccc tgatcgaggc gggcgagccg
     1981 ctcgcctttg gcctcgcggc ggccgagcgc ggcatggtcg gctttcacga catcgtcgtc
     2041 gccccgaaag cgcgcggccg cggcctcgga cgccgcctcg tcggcgcgct gatcgactgg
     2101 ggccggaggc gcggcgcgcc gcaggcctat ttgctggtgc gtgaaggcaa tcgcccggcg
     2161 cgggcgctgt accggtcgct gggctttgtc gaagcgtacc gctacgccaa catggtgagg
     2221 ggatagggac gtactttcct cccccgtgaa gcgaagcgga acgggggagg gggaccgcgc
     2281 gtcagcgcgg tggagggggc cgcgggcggt tgggattagc ctgatagacc ggagccccct
     2341 ccaccatgct tcgcatggtt cccctccccc gctgcgcggg ggaggaaggg gcccaattcc
     2401 ctcaaattcg acctttccgc tcaaccttcc gccagccatg ccgccaggaa tccgggcgca
     2461 aagcccgatt caacctcgct taaccacgga ttttagcgct tcgcgcaacc cggcgcgcca
     2521 tgcttcccgc aacgaaaacg cgtcgaacgg gaagagcggg caagatggag ctggagcggc
     2581 ctttcgccat gagcgccgag cgtttcgcgc cgaagccggg cgcggcggag gcgcggcgca
     2641 ttatcgccgc cgatccgcgc gccgacgggc ggcggcggga agtgatgctg gcgcgcgggc
     2701 gcgtccagat cgcgcgcagc gtcgccggcg ttcggatgaa gatttcgctc gcggcgagcg
     2761 cctatcgcgg cgtgatcttg cgcctgcgcg gcttcgaggc gcagcgcttt tcctacgaga
     2821 tcgagctcgc ccatcgcgat cccgatctca gcgtgacgct gctcgaggcg agcgacgacg
     2881 gcgaggtcca ggccgagtgg cggctctggg cgaggttcct cggcctgccg gctttggtcg
     2941 agcgcgagga aggccggccc gagccggacg ccgcgcggct cggcgaactc gctgtcagcc
     3001 caagaaagcc gcgccgccgc ggcggagcga tggcccggcg gcggccgcgc ttcctgacgc
     3061 gacgcaagac cggccgcggc tcgccggggc cgtcgctcgc cggcgcgcgg gaaatctttc
     3121 caggctcgaa ggacgggcgg tagtcctctc catcattgcg agcgcagcga agcaattcat
     3181 tcgtagggct gtaccttaca aatggattgc ttcgctgcgc tcgcaatgac ggatgcgctc
     3241 acctgaacgg cggctcgtcg aagctcctga gcttgcgcga atgcagcgtc gctctttggc
     3301 cgcgcagcat atccacggcg gcgaggccga tctgcaaatg ctcgccgacg gcgcgctcgt
     3361 agaaggcgtt ggcggcgccg ggcagcttga tctcgccatg caaaggcttg tccgagacgc
     3421 agagcagcgc gccatagggc acgcgcatgc ggaagccttg cgtcgcgatc gtgcccgatt
     3481 ccatatcgac cgcgatggcg cgcgacaggt tgatgcggcg gcgctcctgc gtccagcgca
     3541 attcccaatt gcggtcgtca ttggtgacga cggtgccggt gcgcagccgc cgcttcaagg
     3601 cttcgccgcg gtcgccggtg acttgcgccg ccgcctcctg cagggcgacc tgtacttctg
     3661 ccagagccgg gatcggcatg tccagcggca ccgcgtcgtc aaggatgtga tcctgcctca
     3721 aataggcgtg ggcgagg
//
LOCUS       DATGJW010000292         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_987813, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000292 DATGJW010000000
VERSION     DATGJW010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_987813"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..606)
                     /locus_tag="VKU03_08965"
     CDS             complement(<1..606)
                     /locus_tag="VKU03_08965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLJ71434.1"
                     /translation="MLKSDSTNLRAGGRILVDQLVRQGVEHVFCVPGESYLAALDAMH
                     DASLNVTVCRQEAGATMMAATSAILTGRPGICFVTRGPGATNAAHGVHIAEHDSAPMI
                     LFIGQVERAMMGRGAFQEMDYRSLFASTAKLATEIETAAQIPEVVQRAFHIAMQGRPG
                     PVVIALPEDMQTEMAAVADAPRAEPVPIWPGLTQMARLQKML"
     gene            complement(693..1883)
                     /locus_tag="VKU03_08970"
     CDS             complement(693..1883)
                     /locus_tag="VKU03_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014497235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HLJ71435.1"
                     /translation="MNAAATEFVWDDPLNLEAQLGEDERLIRDQARDYARRKLAPRIV
                     EAYEKERVEPEIFREMGERGLLGVTLPEKYGCAGANYVAYGLIAREVEAVDSGYRSMM
                     SVQSSLVMYPIYAYGDERQREKYLPKLAAGEWIGCFGLTEAEAGSDPGSMKTKAEPIS
                     GGYRLTGSKLWISNAPIADVFVIWAKSSAHGDKIRGFVLEKGMKGLSAPKIEGKLSLR
                     ASVTGEIVLQGVEVPETSLLPNVSGLKGPFGCLNRARYGIAWGAMGAAEDCWRRARDY
                     ALGRKQFGRPLAATQLVQKKLADMQTEIALGLQAALRVGRLMDEGRWAPDMISLIKRN
                     NCGKALDIARLARDIHGANGIVGGYHVMRHAQNLETVNTYEGAHDVHALILGRAQTGL
                     QAFF"
     gene            2068..>2430
                     /gene="hrcA"
                     /locus_tag="VKU03_08975"
     CDS             2068..>2430
                     /gene="hrcA"
                     /locus_tag="VKU03_08975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177450.1"
                     /note="Negative regulator of class I heat shock genes
                     (grpE-dnaK-dnaJ and groELS operons). Prevents heat-shock
                     induction of these operons; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heat-inducible transcriptional repressor HrcA"
                     /protein_id="HLJ71436.1"
                     /translation="MSDPGAATNLDARAREIFRRIVDSYLATGEPVGSRHLSRILPIS
                     LSPASVRNVMQDLEELGLVYAPHTSAGRMPTELGLRFFVDALLELGDVAAADRGRIDA
                     EVSAAVRSGRIEGALTEAT"
BASE COUNT          396 a          816 c          783 g          435 t
ORIGIN      
        1 tagcatcttc tgcaatctgg ccatttgcgt aagccccggc cagattggga ccggctcggc
       61 gcgcggggcg tccgcgaccg cggccatttc ggtctgcatg tcctccggca gcgcgatcac
      121 aaccgggccc ggccgcccct gcatggcgat gtggaacgcg cgctggacaa cctccgggat
      181 ttgcgccgcg gtctcgatct cagtcgcgag cttcgcggtc gacgcgaaca gcgagcgata
      241 gtccatctcc tggaacgcgc cgcggcccat catcgcccgc tcgacttgcc ctatgaacag
      301 gatcatcggc gccgaatcgt gctcggcgat atgaacgcca tgcgccgcgt tggtcgcgcc
      361 ggggccgcgc gtgacgaagc agattcccgg ccttccggtc aggatcgcgc tcgtcgcggc
      421 catcatcgtc gcgccggcct cctggcggca gaccgtgacg ttgaggctcg cgtcatgcat
      481 cgcgtcgagc gcggcgaggt acgattcgcc aggcacgcag aagacgtgct cgacgccctg
      541 gcggacgagc tgatcgacga ggatgcgccc gccggcgcgc agattggtgg aatcgctctt
      601 caacattccc tcgaaacctt gccggttacc ttaaggtgat gaccatgacc gaaacgccgt
      661 cctcgccggc gaagcgacga aacgctttcg catcaaaaga acgcctgcaa gccggtctgc
      721 gcgcggccga ggatcagcgc atggacgtca tgcgcgcctt catacgtatt gacggtctcc
      781 aggttctgcg catggcgcat cacatggtag ccgccgacaa tgccgttggc gccgtgaatg
      841 tcgcgggcga ggcgggcgat gtcgagcgcc ttgccgcaat tgttgcgctt gatcagcgag
      901 atcatgtcgg gcgcccagcg gccttcgtcc atcaggcggc cgacgcggag cgccgcctgc
      961 agcccgaggg cgatttcggt ctgcatgtcg gcgagctttt tctggacgag ctgcgtcgcg
     1021 gcgagcggcc ggccgaattg cttgcggccc aaggcatagt cgcgggcgcg ccgccagcaa
     1081 tcctccgccg cgcccatcgc gccccaggcg atgccgtagc gcgcccgatt gaggcagccg
     1141 aacgggccct tcaggccgga cacattcggc agcaagctgg tttctgggac ttcgacgcct
     1201 tgcaacacga tttcgccggt caccgaggcg cgcagcgaca gcttgccctc gatcttgggg
     1261 gccgagaggc ccttcatccc cttttcgagc acgaatccgc gaatcttgtc gccatgcgcc
     1321 gaagatttcg cccagatgac gaagacgtcg gcgatcggcg cgttggagat ccagagcttc
     1381 gagccggtga gacgatagcc gccggaaatc ggctcggcct tggtcttcat cgagccggga
     1441 tcggagccgg cttccgcctc ggtgaggcca aaacagccga tccactcgcc ggcggcgagc
     1501 ttgggcagat atttttcgcg ctgcctctcg tcgccatagg cgtagatcgg atacatgacg
     1561 agcgaggatt gcacgctcat catcgagcga tagccggaat cgaccgcttc gacctcgcgc
     1621 gcgatcaggc cgtaggcgac gtaatttgcg ccggcgcagc cgtatttttc cggcaacgtc
     1681 acgccgagca ggccgcgctc gcccatttcc cggaagattt ccggttcgac cctctccttt
     1741 tcataggctt cgacgatgcg cggcgcgagc ttccgccgcg cgtagtcgcg ggcttggtcg
     1801 cggatgaggc gctcgtcttc gcccaattga gcttcgagat tcagcgggtc gtcccagacg
     1861 aactctgtcg cggcggcgtt catcggcaag cctctcttaa gaccctttgg cggcggattt
     1921 cgcgcgacgc gtcgaagcaa gagccagcca agacctatgg ccccggagcg gaccttattc
     1981 aagcccgcgc cgattggcgg cggccggctt gcgcctgagc ggccgagcgg ccattttata
     2041 gcggtgaccg tcccgaacga gaaaatattg tccgacccgg gcgccgcgac gaatctcgac
     2101 gcgcgagcgc gcgagatctt tcgccgcatc gtcgattctt acctggcgac cggggagccg
     2161 gtcggctcgc gccatctctc gcgcattctg ccgatctcgc tgtcgccggc ctcggtacgc
     2221 aacgtgatgc aggatctcga ggagctcggc ctcgtctacg cgccgcatac cagcgccggc
     2281 cgcatgccga cggaattggg cttgcgcttc ttcgtcgacg cgctgctcga actcggcgat
     2341 gtcgccgccg ccgatcgcgg ccgcatcgac gccgaagtga gcgccgccgt caggagcggc
     2401 cggatcgaag gcgctctgac cgaggcgacc
//
LOCUS       DATGJW010000293         2741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1300581, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000293 DATGJW010000000
VERSION     DATGJW010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2741
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1300581"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..667
                     /locus_tag="VKU03_08980"
     CDS             <1..667
                     /locus_tag="VKU03_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HoxN/HupN/NixA family nickel/cobalt transporter"
                     /protein_id="HLJ71437.1"
                     /translation="AIFLLAIAAMNIVIFFSIYESYRRVRAGGAYIEGDLDVLLNSRG
                     FLARLFRPLFKLVTKSWHMLPLGFLFGLGFDTATEVAVFGVSAAQAAKGAPLATILVF
                     PVLFAAGMSLVDTADGVMMLGAYRWAFVKPMRKLYYNMTITLVSVVAALLIGGVEALG
                     LIGDKLELKGAFWDAVSGLNDNFNGLGFAIIGVFFLAWLVSYAIYKAKKLDAFEIAGG
                     GPI"
     gene            679..1074
                     /locus_tag="VKU03_08985"
     CDS             679..1074
                     /locus_tag="VKU03_08985"
                     /inference="COORDINATES: protein motif:HMM:NF019460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1636 domain-containing protein"
                     /protein_id="HLJ71438.1"
                     /translation="MSENRRGALHRLIICTTCRLEGSPADGPSDGGKLYAMALAMSET
                     WADGQGCVVSGVECMSGCNRSCTIGLAAPAKPSYLFGGLAPTAETAADALALVAQYRD
                     KADGVLERRTRPPSFRRGILAKIPAPFAS"
     gene            1071..1721
                     /gene="bluB"
                     /locus_tag="VKU03_08990"
     CDS             1071..1721
                     /gene="bluB"
                     /locus_tag="VKU03_08990"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008874728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HLJ71439.1"
                     /translation="MSGSGAPLFDDAFRARLETLIRWRRDVRRFKPDPIAPDLVEDLL
                     RLAALAPSVGLSQPWRFVSVDDPARRAAVRRNFEAANAEALQACPDERKALYAKLKLA
                     GLDEAPRHLALFAEAQPEQGHGLGRRTMPETVAYSAVMAAHTLWLAARAYGIGIGWVS
                     ILDPAAIAEALDVPSAWLFIGYFCLGYPQAEDDIPDLERRGWERRREADKVALLQR"
     gene            complement(1858..>2741)
                     /locus_tag="VKU03_08995"
     CDS             complement(1858..>2741)
                     /locus_tag="VKU03_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042242 - cobyrinic acid a,c-diamide
                     synthase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobyrinate a,c-diamide synthase"
                     /protein_id="HLJ71440.1"
                     /translation="IGGIILNRVGSERHKRLAAEAIARTSLPVLGALPRSAEIVLPER
                     HLGLVQAGETGDLDAVLDRLADFVAAHVDLDAVIALAKRTAATAATRPALAPPGQRIA
                     VARDAAFSFLYPHLEQAWRKAGAELAFFSPLADEAPAPDCDFCWLPGGYPELHAGRLA
                     AARNFLQGVKRFAATKPVHGECGGYMVLGSGLIDADGVRHEMAGLLGLVSSFAKRRMT
                     LGYREATLRADHPLGRTGARHRGHEFHYATIVEPGGDEPFADVADAYGSAPAPAGGRR
                     RLVSGSFFHLIAPCENG"
BASE COUNT          412 a          921 c          941 g          467 t
ORIGIN      
        1 ggcgatcttt ctgctcgcca tcgccgcgat gaacatcgtc atcttctttt cgatctatga
       61 gagctatcgg cgcgtgcgcg ccggcggcgc ctatatcgag ggcgatctcg acgtgctgct
      121 gaacagtcgc ggctttctcg cccgactgtt ccggccgctg ttcaaactgg tgacgaaaag
      181 ctggcatatg ctgccgctcg gctttctgtt cgggctcggc ttcgacaccg cgacggaagt
      241 tgcggtgttc ggcgtctcgg cggcgcaggc cgcgaaaggc gcgccgctcg cgacgatcct
      301 tgtctttccg gttctgttcg cggccggcat gtcgctcgtc gatacggccg acggcgtgat
      361 gatgctcggc gcctatcgct gggctttcgt caagccgatg cgcaagctct actacaacat
      421 gacgatcacg ctggtctcgg tggtcgccgc gctgctgatc ggcggcgtcg aggcgctcgg
      481 cttgatcggc gacaagctcg aattgaaggg cgccttctgg gacgccgtgt ccggactgaa
      541 cgacaatttc aacggcctgg gcttcgccat tatcggcgtc ttcttcctcg cctggctcgt
      601 ctcctatgcg atctataagg ccaagaaact cgacgccttc gaaatcgcgg gcggcggccc
      661 gatctgagga agccggccat gagcgaaaac cggcgcggcg ccttgcatcg gttgatcatc
      721 tgcacgacct gccggctcga aggctcgccg gccgacgggc cgagcgacgg aggcaagctc
      781 tatgcgatgg cgctggccat gtccgaaacc tgggccgatg gccagggttg cgtggtgagc
      841 ggcgtcgaat gcatgagcgg ctgcaaccgc tcctgcacga tcggcctcgc cgctcccgcc
      901 aagccgagct atctcttcgg cgggctggcg ccgacggccg agaccgcggc ggacgccctg
      961 gcgctcgtcg cgcaatatcg cgacaaggcc gacggcgtat tggagcggcg gactcgtccg
     1021 ccgagcttcc gccgcggcat tctggccaag attccggcgc ctttcgcgtc atgagcggga
     1081 gtggcgcgcc tcttttcgac gacgcgttcc gggcgcggct ggaaacgctg atccgctggc
     1141 ggcgcgacgt aaggcgcttc aagcccgatc cgatcgcgcc agacctcgtc gaggatttgc
     1201 tgcgcttggc ggcgttggcg ccctcggtcg ggctcagcca gccttggcgg ttcgtcagcg
     1261 tcgacgaccc ggcccgccgc gcggcggtgc ggcgcaattt cgaggcggct aacgccgagg
     1321 cgctccaagc ctgccccgac gagcgcaaag cgctctacgc caagctgaag ctcgccggcc
     1381 tcgacgaggc gccgcgccat ctcgcgctgt tcgcggaggc tcagcctgag cagggccatg
     1441 gcctcggccg ccgcaccatg cccgagaccg tggcctattc ggcggtgatg gcggcgcata
     1501 ctctatggct cgccgcccgc gcctacggga tcggaatcgg ctgggtgtcg atcctcgatc
     1561 cggcggcgat cgcggaggcg ctcgacgtac cgtccgcctg gctgttcatc ggctatttct
     1621 gccttggcta tccgcaggcg gaggacgata tccctgacct tgagcggcgc ggctgggagc
     1681 ggcggcgcga ggccgacaag gtcgctttgc tgcagcggta ggcgccgccg ccgaaccaag
     1741 ggggcggatc gcgctcatcc gcttcgctcg acgcagcgag tcgcctgttt cgtgcaccgc
     1801 tgtaccgaac attgattgct tcgcgccgct cgcaatgacg tcgtttgcga gcgcgcttca
     1861 gccgttctcg catggcgcga tgagatggaa gaagctgccc gagacgaggc ggcgccggcc
     1921 gccggccggc gccggggccg agccataggc gtccgcaacg tcggcgaatg gctcgtcgcc
     1981 gccgggctcg acgatcgtcg catagtggaa ttcatgaccg cgatggcgcg cgcctgtgcg
     2041 ccccaaagga tggtcggcgc gcagcgtcgc ctcgcgatag ccgagcgtca tgcggcgctt
     2101 ggcgaacgaa ctgacaaggc cgagcaggcc ggccatttcg tggcggacgc cgtccgcgtc
     2161 aatcaagccg ctcccgagaa ccatgtagcc gccgcattcg ccatggaccg gcttagtcgc
     2221 ggcgaagcgc ttgacgccct gcaggaagtt ccgcgccgcc gccaatcggc cggcatggag
     2281 ttcgggatag ccgcccggca gccagcagaa atcgcaatcc ggcgccggcg cctcgtcggc
     2341 aagcggcgag aagaaggcca gctccgcgcc ggctttccgc caggcctgtt cgagatgcgg
     2401 atagagaaac gagaaggcgg cgtcgcgcgc gacggcgatt ctctgtcccg gcggcgcgag
     2461 cgccggccgc gtcgccgccg tcgccgccgt ccgcttggcg agcgcgatga ccgcgtcgag
     2521 atcgacatga gcggcgacga aatcggcgag gcggtcgagg accgcgtcga gatcgcccgt
     2581 ctcgcccgcc tggacgaggc cgagatggcg ctccggcagc acgatctcgg ccgagcgcgg
     2641 caatgcgccg agaacgggca gcgaggtccg cgcgatcgct tcggccgcga gtcgcttatg
     2701 ccgctcgctg ccgacccgat tgaggataat tccgccgatc t
//
LOCUS       DATGJW010000294         3108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_782768, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000294 DATGJW010000000
VERSION     DATGJW010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3108
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_782768"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(162..1550)
                     /locus_tag="VKU03_09000"
     CDS             complement(162..1550)
                     /locus_tag="VKU03_09000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017996352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ71441.1"
                     /translation="MDYAKPEQFLHPKDQPKRHGLALPTLCAAVLVAQIDTSVVNLGA
                     RPIGAAFNAPVEALQWVVDSYNLVYAALLLTGGLLADLVGRRRVFMTGAAVFTAASLV
                     CAFAPAISILIAGRALAGLGAALLLPSSLAIIRVAWPNPSERGRVLGIWTGCNGVALA
                     IGPTLGGWLIAGFGWRSIFLIVVPLGLAAFIAALPAVPESSDPQNRAFDAAGQIFGAV
                     ALAGIAVAAIESRRAVLAAAAALTVAAVAFAFFVRAERRRGAAALVPLGLFGIAEFRG
                     AATATVGMTFGMYGLLFLLPLFWLSAGVLSATAAGLALTPSAIAYVLTSPFSGALSER
                     LGARLMTAGGVAVIGAGLLLVAATASAASILGAGIGLALTGIGMGMATGPLMAVAVGA
                     VAAARSGTAAALINVARMAGATIGVAVLGAAYALIGGGVGGLRLAMALGGAAQVAAAA
                     IAWRTTPREGTR"
     gene            1701..2405
                     /locus_tag="VKU03_09005"
     CDS             1701..2405
                     /locus_tag="VKU03_09005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007563470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ71442.1"
                     /translation="MSLLPSFAAIAALIADPARAAMLIAVLDGRALPAGELAYAAGIT
                     AQTASAHLAKLLDGGLLSVETCGRHRYYRLAGAHIAQALEQLAAIRPAEPVRRKALTP
                     EARELRFARCCYDHLAGQLGVAATRAMQEKGFIVAADEKRFEVTAAGAEWFGGLGLNV
                     ASLKPTRRGLARQCLDWTERSHHLAGPLGVAFLDRLCAAGWLRRSRQSRLVRVTPKGR
                     IALKERLGVEAEPLPT"
     gene            2684..>3108
                     /locus_tag="VKU03_09010"
     CDS             2684..>3108
                     /locus_tag="VKU03_09010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HLJ71443.1"
                     /translation="MADDELDLSTLSDDELVEQMHDDLYDGLKEEIEEGVRILLSRRW
                     TPYDILTKALVEGMRIVGIDFRDGILFVPEVLMSANAMKGGMAILRPLLAETGAPKMG
                     KMVIGTVKGDIHDIGKNLVCMMMEGAGFEVIDLGINNPV"
BASE COUNT          560 a         1064 c         1044 g          440 t
ORIGIN      
        1 aacaccttga ggccgcgcct gctttttctg agtctcgaca acagaaattc gatgaagaaa
       61 tctcgaaacc ttcttgtatt gcgatatcgc cgcctgcagc aactcgattc atgctcactt
      121 tccccggaca gccccccgca aggcggggag aaggggttcg cctatctcgt accttctcgc
      181 ggcgtcgtgc gccaggcgat cgcggcggcg gcgacttgcg ccgcgccgcc cagcgccatc
      241 gcgaggcgca gcccgccgac gccgccgccg atcagcgcat aggcggcgcc gagaacggcg
      301 acgccgatcg tcgcgccggc catgcgcgcg acattgatca gcgccgccgc cgtgcccgat
      361 cgcgcggcgg cgaccgcgcc gaccgcgacc gccatcaaag gcccggtcgc catgcccatg
      421 ccgatcccgg tcagcgccaa gccgatcccg gcgccgagga tcgacgccgc cgaggcggtc
      481 gcggccacca gcagcagccc ggcgccgatc accgcgaccc cgcccgccgt catcaaccgc
      541 gcgccgagcc gttcgctgag cgctcccgaa aacggcgagg tcaggacata ggcgatagcc
      601 gaaggcgtca gggcgaggcc ggccgccgtc gcgctcaaga cgccggcgct gagccagaac
      661 agcggcaaga ggaacaagag accgtacatg ccgaacgtca tgcccactgt cgcggtggcc
      721 gcgccgcgga attcggcgat gccgaacagg cccaaaggga cgagcgccgc ggcgccgcgc
      781 ctgcgctcgg cccgaacgaa gaaggcgaaa gcgacggccg cgaccgtcaa agcggccgcc
      841 gccgcgagca cggcgcggcg cgactcgatc gccgcaacgg cgattccggc cagcgcgacg
      901 gcgccgaaga tctggccggc ggcgtcgaaa gcgcgattct gcggatcgga cgattccgga
      961 accgccggca aagccgcgat gaaagccgcc aagccgagcg ggacgacgat caggaaaatg
     1021 ctgcgccagc cgaatccggc gatcagccag ccgcccaaag tcggcccgat cgccaaggcg
     1081 acgccgttgc agcccgtcca aatgccgaga acccggccgc gctccgaagg atttggccag
     1141 gcgacgcgga tgatcgcgag cgaggagggc agcagcagag cggcgccgag cccggccagc
     1201 gcgcggccgg cgatcaggat cgagatcgcg ggcgcgaagg cgcagacgag cgacgccgcg
     1261 gtgaaaacgg ccgcgccggt catgaagacg cggcggcgcc cgacgagatc ggccaaaagg
     1321 ccgcccgtca aaagcaaagc ggcgtagacg agattgtagc tgtcgaccac ccattgcagg
     1381 gcttcgaccg gggcgttgaa agcggcgccg atcggccgcg cgccaaggtt gacgacggac
     1441 gtgtcgatct gcgccaccag caccgcggcg cataaggtcg gcagcgccag cccatggcgc
     1501 ttcggctgat ccttcgggtg gagaaactgc tctggcttgg cgtagtccat ggcgcggctc
     1561 cgcggagaag gtgcgacgcg cgatcggcgt cccgttggct ggtcatcatc gtccccgccg
     1621 cgccgcgatg cttcggccgc gatcgaagcg ttcagccggc ggatgggtta agaaggcgcc
     1681 acaaaaccgg agaagagcct atgtctctct tgccgagctt cgccgccatc gccgcgctga
     1741 tcgccgatcc agcccgcgcc gcgatgctga tcgccgtgct ggacggccgc gctttgccgg
     1801 ccggcgagct ggcttacgcc gccgggatca ccgcccagac ggccagcgcg catctcgcca
     1861 agctgctcga cggcggcctg ctcagcgtcg aaacctgtgg ccggcatcgc tattaccggc
     1921 tcgccggcgc tcacatcgcg caagctttgg agcagctcgc cgccatccgg ccggccgagc
     1981 cggtccgacg caaggccttg acgcccgagg cgcgcgaatt gcgcttcgcg cgctgctgct
     2041 atgaccacct ggccggccaa ttgggcgtcg cggcgacccg cgccatgcag gagaagggct
     2101 ttattgtcgc cgccgacgag aagcggttcg aggttaccgc cgccggcgcc gagtggttcg
     2161 gcggcctcgg cctgaatgtc gcctccttga agccgactcg gcgcggcctt gcccggcaat
     2221 gcctggattg gaccgagcgc agccatcacc tcgccgggcc gctgggcgtc gcctttctcg
     2281 acaggctttg cgccgccggc tggttgcgcc gctcgcgcca gtcgcgcctc gttcgcgtga
     2341 cgccgaaggg ccgcatcgcg ctgaaggagc gcctcggcgt cgaagctgag ccgctcccaa
     2401 catagacgct gagaagcgag tcggcctggc taaagaatat gtcggaatcg gacgggcggc
     2461 gttcgacggc aatcttattc ttcaccggtg aggccgtccg aggcggcgtt ccgccaaatt
     2521 cttggacggc atgcgaaaaa ttgggcaacc gcttgcgata attgcgcttg gcgcgaccgg
     2581 cgtccctcgc cgatagtgaa gccgctcgcc tcggcgtcgc cgaggcgttt tggcgttggc
     2641 ggcgtgacgg ggcgccggct cgcgaaatga acgggaaggc gctatggccg acgacgaact
     2701 cgatctttcg acgctctccg acgacgaact cgtcgagcag atgcatgacg atctctatga
     2761 cggcctcaaa gaagagatcg aagagggcgt gcgcatcctg ttgtcgcgcc gctggacgcc
     2821 atacgacatc ctgaccaagg ctctggtcga aggcatgcgc atcgtcggca tcgattttcg
     2881 cgacggcatt cttttcgtgc cggaagtgct gatgtcggcc aacgcgatga aaggcggcat
     2941 ggcgatcctc cgcccgcttc tcgccgaaac cggcgcgccc aaaatgggca agatggtgat
     3001 cggcacggtc aagggcgaca tccacgacat cggcaagaac ctcgtttgca tgatgatgga
     3061 gggcgccggt ttcgaagtca tcgatctcgg catcaacaat cccgtcga
//
LOCUS       DATGJW010000295         4673 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_886108, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000295 DATGJW010000000
VERSION     DATGJW010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4673)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4673)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4673
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_886108"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VKU03_09015"
     CDS             complement(<1..449)
                     /locus_tag="VKU03_09015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004685900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ71444.1"
                     /translation="MNDLLTAAAAGLAAAGAYALLGVCTVFTYRLVAVVNFTGAAIGA
                     AGAFVFVTLAEAGAPLLPSAVAGIAFGALLGVAIGFVMTRWFAGANASTKAAVTVALL
                     VGIVAIGLRLTGGQHTHSFPDLVPGAAFRLAGVEVTNAAIATLALALL"
     gene            complement(514..1686)
                     /locus_tag="VKU03_09020"
     CDS             complement(514..1686)
                     /locus_tag="VKU03_09020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006146722.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ71445.1"
                     /translation="MRLTFVAALAIAGAFGLPGAALAAGPTCPNGEIELGAVSTITGP
                     ADFSEVPKATQAVFDEVNAAGGINGCKIKYTIEDDKADPQVAAQAARDLIDNKKVIGF
                     VGSASLLDCAVNGATYKRNNVRSIQGLGVDPACFSSPNIAPVNVGPYTLTTAMGLFAV
                     DTLKSKKFCAFFIIIGGTQEAYKNAIAEWEKITGKKIGLIDLTLPLQGDLTPYLIKAR
                     DSGCDATISNAVEPGVVQWVKTADAQKITGVNWLFLAPGYTTEVAKALASSKQPVYAG
                     TEWEPYTEANSAANKDWIATMNAAKRPLTAFSQGGYLAAEMFIDVLKTIDGPVTRDSF
                     NKALLTMKPISQPLSGTPYVFGPGDKHAPMQATKVMKLENGAWTVETPNWVKLPKM"
     gene            complement(1711..2829)
                     /locus_tag="VKU03_09025"
     CDS             complement(1711..2829)
                     /locus_tag="VKU03_09025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLJ71446.1"
                     /translation="MSAREAALIPVTLLTGFLGSGKTTLLNRLLRAPALAGAAVIVNE
                     FGEVGLDHLLIETSEERFALLDNGCLCCTVRGDLVDTLKSLLDRRVGGAPAAPTRVLI
                     ETTGLADPAPILHTLIADATLAAHFRVDSVTTTVDAVNGENTLAAHPEAIKQVAVADH
                     LLLTKTDLVAAPALDRLRRQLRAINPAARLRACRNGEIAAKTVFGGGFSSLSGRSAET
                     VAWFRQTTAAIEREARRAAHGAECADPACRHPAHGLRYEPGHAARVNTFSIVIAEPLA
                     WTAFSQWLEYLAAMKGEDLLRFKGLVHIAEKPETPIVVHGVQHVFHPPRELSGWPSGD
                     RRTRLVFITRDIPRRLIEDTLSKFGAIARSRIERPVAA"
     gene            complement(2816..4471)
                     /locus_tag="VKU03_09030"
     CDS             complement(2816..4471)
                     /locus_tag="VKU03_09030"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002972238.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLJ71447.1"
                     /translation="MTDRNGVDLVLRNGRFYTANRNQPWARAVAIKAGRIVALDADAE
                     NRPAEKSVDLSGRMAMPGVIDVHTHMMMGGQSELFELRFPSHSSLAETCAYVRERAER
                     AAAGAWIVGAQWGSDMLPRLNTAAALAALDEASLGHPVLLRDDTHHNRWCSSEALRRA
                     GVTAATANPNRGEIGRDPATGALTGVMVETAAGLVERALAESGHYTPEMDRAAMARSI
                     ATLNAYGVTAFLDAASMQPILAALKGLDDRGELTAWAGCSMPAVEPSFMFGLAGDALL
                     DLRETYRSAHVKPDFVKVFLDGVPGFKTAAFHDAYKPDPIRGCCFRGSTLLTVPDLVR
                     WIGKCEKRGLSVKIHCAGDAAVTQALDAIDVARSFNGPTDLTHHIAHASYIAPQDIRR
                     FAELGVVADLSPIIWYPTHFLEGHKLAMDEARAERFWPMKDLAEAGALMAAGSDWPVI
                     PNPDPWNGMEGMVTRRNPSGEFGDRALWPEQALDLATVLEIYTINAARAIGLAGVTGS
                     LEIGKSADVIVLERNLFDIPADEIADTRVLTTYFEGRPVYERA"
     gene            complement(4468..>4673)
                     /locus_tag="VKU03_09035"
     CDS             complement(4468..>4673)
                     /locus_tag="VKU03_09035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71448.1"
                     /translation="VKTHLIRIFDKLGVENRAAAVALLAGCRDLNVNQTVDCAGFAPR
                     HIAHASDGATRFAARGLMETSVS"
BASE COUNT          831 a         1492 c         1549 g          801 t
ORIGIN      
        1 agaagcgcaa gggccagagt ggcgatcgcc gcattcgtca cctcgacgcc ggcaaggcgg
       61 aaggccgcgc ccggaacgag gtcgggaaaa ctatgcgtgt gctgcccgcc ggtgaggcgc
      121 aggccgatcg cgacgatgcc gacgagcaac gccacggtga ccgcggcctt ggtcgaggcg
      181 ttggcgccgg cgaaccagcg cgtcatgaca aagccgatgg cgacgccgag caaggcgccg
      241 aaggcgattc cggcgaccgc cgagggcagc agcggcgcgc cggcttcggc gagggtcacg
      301 aagacgaaag cgcctgccgc gccgatcgcc gcgccggtga aattgaccac cgccacaaga
      361 cgataggtga aaaccgtaca gacgccgagc agcgcatagg cgccggcggc cgccaatccc
      421 gccgccgccg cggtcaacaa atcattcatc gctcaagccg tctcggacgg gacgccggcg
      481 aggcggcggc tttccgccgc ctcgccgcgt ttctcacatt ttcggcagct tgacccaatt
      541 cggagtctcg acggtccaag cgccgttttc aagtttcatc accttggtcg cctgcatcgg
      601 cgcatgcttg tcgcccgggc cgaacacata gggtgttccg gacagaggct gggatatcgg
      661 cttcatggtc agaagcgcct tgttgaagct gtcgcgcgtc accgggccgt cgatcgtctt
      721 cagcacgtcg atgaacattt cagccgcaag ataaccgcct tgcgaaaatg cggtcaaagg
      781 ccgcttggcc gcgttcatcg tcgcgatcca gtccttgttg gcggcggaat tcgcctcggt
      841 ataaggctcc cactccgtgc cggcatagac cggctgcttg ctgctggcga gcgccttggc
      901 gacctcggtc gtatagccgg gcgcgaggaa cagccaattg acgccggtga tcttctgcgc
      961 gtcggcggtt ttcacccatt gcacgacgcc gggctcgacg gcgttggaga tcgtcgcgtc
     1021 gcagcccgaa tcccgcgcct tgatcagata gggcgtcaag tcgccttgca gcggcagcgt
     1081 caggtcgatc aggccgatct tcttgccggt gatcttctcc cattccgcga tggcgttctt
     1141 gtacgcctcc tgcgtgccgc cgataatgat gaagaaggcg cagaattttt tcgatttcag
     1201 cgtatcgacg gcgaagagcc ccatcgccgt cgtcagcgtg taggggccga cattgaccgg
     1261 cgcgatattc ggcgaggaga aacaggccgg gtcgacgcca agcccctgga tggagcgcac
     1321 attgttgcgc ttgtaggtcg ccccgttgac cgcgcaatcg agcaggctgg ccgagccgac
     1381 gaagccgatg accttcttgt tgtcgatgag atcgcgcgcg gcctgggcgg cgacttgcgg
     1441 atcggcttta tcgtcttcga tcgtatattt gatcttgcag ccattgatgc cgccggcggc
     1501 gttaacctca tcgaacacag cctgggtcgc cttcggcact tcgctgaaat cggccgggcc
     1561 ggtaatggtc gacacagccc cgagctcgat ctcgccgttc ggacaggttg gtccggcggc
     1621 caaagccgct cccggcaggc cgaaggcgcc tgctatcgca agcgccgcaa cgaatgtcag
     1681 tctcatcttg tcctcccttg cttgattttc tcaagcggcg accggccgct cgatgcggct
     1741 tcgcgcgatc gcgccgaatt tcgacaaagt gtcctcgatc aggcgacgcg gaatgtcgcg
     1801 cgtgatgaag acgagccgtg tgcgccgatc gccgctcggc catccggaaa gctcccgcgg
     1861 cggatgaaag acatgctgca cgccatgaac gacgatcggc gtttcaggct tctcggcgat
     1921 atggacaaga cctttgaagc gcagcaagtc ctcgcccttc atcgcggcga gatattcgag
     1981 ccattgcgaa aacgccgtcc aagccagcgg ctcggcaatg acgatcgaga acgtattgac
     2041 gcgcgcggca tgacccggct cgtaccgcaa tccatgcgcc ggatggcggc aggccggatc
     2101 ggcgcactcc gccccatgcg ccgcccgcct cgcctcgcgc tcgatcgccg ccgtggtctg
     2161 gcggaaccag gcgacggtct cggctgatcg ccccgagaga cttgaaaagc cgccgccgaa
     2221 aaccgttttc gcggcgatct cgccattgcg gcaggctctg agcctcgccg cagggttgat
     2281 ggcgcgcaat tgccggcgca ggcgatcgag cgccggcgcc gcgacgagat cggtcttggt
     2341 caggagaaga tgatcggcga cggcgacttg cttgatcgcc tcgggatgag cggcgagcgt
     2401 gttctctcca ttgaccgcgt cgaccgtcgt cgtcacgctg tcgacgcgaa aatgcgcggc
     2461 gagcgtcgcg tcggcgatca gcgtatggag gatcggcgcc ggatcggcga gaccggtcgt
     2521 ctcgatgagg acgcgcgtcg gcgcggcggg ggcccctcca acgcggcgat cgagaagcga
     2581 tttcagcgtg tcgacgagat cgccacgcac cgtgcagcag aggcagccat tgtcgagcag
     2641 cgcgaagcgc tcttcgctcg tctcgatcag caaatgatcg aggccgacct cgccgaattc
     2701 gttgacgatg accgccgcgc cggccaaagc gggggcgcgc aacagccggt tgagcagcgt
     2761 cgtcttgccg ctgccgagaa agccggtcag cagcgtgacc ggaatgaggg cggcctcacg
     2821 cgcgctcata gacgggccgt ccctcgaaat aagtggtcaa gacccgcgta tcggcgatct
     2881 cgtcggcggg tatgtcgaaa aggttgcgtt cgaggacgat gacatcggcc gatttgccga
     2941 tttccaaaga gccggtgacg ccggcaaggc cgatcgcccg cgccgcattg atcgtgtaaa
     3001 tttcgagaac cgtggcgaga tcgagcgcct gttccggcca caaagcgcga tcgccgaatt
     3061 cgccggacgg attgcgcctg gtcaccatgc cttccattcc gttccacggg tcgggattcg
     3121 gaatgaccgg ccagtcggag cccgccgcca tcagcgcgcc ggcttcggcg aggtccttca
     3181 tcggccagaa ccgctcggcg cgcgcttcgt ccatggcaag cttatggcct tcgaggaaat
     3241 gggtcggata ccagatgatc ggcgaaaggt cggcgacgac gccgagttcg gcgaagcgcc
     3301 ggatgtcttg cggcgcgata tagctggcat gggcgatgtg atgggtgagg tcggtcggcc
     3361 cattgaagct gcgcgcgaca tcgatcgcgt cgagcgcctg ggtcaccgcg gcgtcgccgg
     3421 cgcaatggat cttcaccgag aggccgcgct tttcgcattt gccgatccag cgcaccaggt
     3481 ctggtacggt caggagggtc gagccgcgga agcagcagcc gcggatcgga tcgggcttgt
     3541 aggcgtcgtg gaaagccgcg gtcttgaagc cgggcacgcc gtcgaggaat accttgacga
     3601 aatccggctt cacatgcgcg ctgcgatagg tctcgcgtaa gtcgagcaag gcgtcgccag
     3661 caaggccgaa catgaaggaa ggttcgaccg ccggcattga gcatcccgcc caagccgtca
     3721 attcgccgcg gtcgtcaagg cctttcagcg ccgcaaggat cggctgcatc gaagccgcat
     3781 cgagaaaggc ggtcacgccg taggcgttca acgtggcgat ggatcgcgcc atggctgcgc
     3841 gatccatttc cggcgtgtaa tgacccgact cggcgagcgc gcgctcgaca aggccggccg
     3901 ccgtctcaac catgacgccg gtgagcgcgc cggtcgcagg atcgcggccg atttcgcccc
     3961 tgttcggatt ggccgtcgca gcggtgacgc cggcgcggcg cagcgcctcc gaactgcacc
     4021 agcggttgtg atgggtgtcg tcgcgcagca gaaccggatg gccgagcgag gcttcgtcaa
     4081 gcgccgccag cgccgccgcc gtattgaggc gaggcagcat gtccgagccc cattgcgccc
     4141 ccacgatcca ggcgccggcc gccgccctct cggcccgctc gcgcacatag gcgcacgtct
     4201 cggcgaggct cgaatgcgag gggaagcgca attcgaacag ctcgctctgg ccgcccatca
     4261 tcatatgggt atgtacgtcg atgacgcccg gcatcgccat gcggccggaa aggtcgaccg
     4321 acttttcggc cggacgattc tcggcgtccg cgtcgagcgc gacgatgcgg cccgctttaa
     4381 tcgccaccgc gcgggcccaa ggctggttcc ggttcgcggt atagaagcgg ccgttgcgca
     4441 gaacgagatc gacgccgttc ctgtccgtca tgaaacgctt gtctccatca ggccgcgcgc
     4501 ggcgaagcga gtcgccccgt ccgaagcatg ggcaatatgg cgcggcgcga agccggcgca
     4561 gtccaccgtt tggttgacat taaggtcgcg gcagccggcc agaagcgcga ccgcggcggc
     4621 gcggttttcg acgccgagct tgtcgaagat ccggataaga tgagtcttca cgg
//
LOCUS       DATGJW010000296         7629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1301397, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000296 DATGJW010000000
VERSION     DATGJW010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7629
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1301397"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            31..354
                     /locus_tag="VKU03_09040"
     CDS             31..354
                     /locus_tag="VKU03_09040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020397359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ71449.1"
                     /translation="MDDDDTIFRALAEASRRRLLDLLHARNGRTLGELRRSLGASRQA
                     VAKHLAVLEGADLVTFLRLGREKLHFINPAPINAIAERWINKFDRGRIAALSDLKRNL
                     EGEDP"
     gene            351..809
                     /locus_tag="VKU03_09045"
     CDS             351..809
                     /locus_tag="VKU03_09045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018480963.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC family protein"
                     /protein_id="HLJ71450.1"
                     /translation="MTSQARSQFVYVTYIRTTADRLWSALTSTEFSQKYWLGARTEAE
                     WRKGGAWRIEFPDGRLADSGEIVEFEPAKRLAIRWRNEWSPELKDEGWSLCTMEIEPA
                     AGESVKLTVTHAIERENSKFIGAVSGGWPQILSNLKSLLENGAAILPPKY"
     gene            complement(947..1999)
                     /locus_tag="VKU03_09050"
     CDS             complement(947..1999)
                     /locus_tag="VKU03_09050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593991.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alcohol dehydrogenase"
                     /protein_id="HLJ71451.1"
                     /translation="MRSYQVVDFGAPLVEAEAPTPKPQGAEALLEVVAAGVCHSDLHI
                     WEGGYDLGRGKRLSLKDRGINLPLTMGHETAGRVVALGPDAKGVAIGRTYLVYPWIGC
                     GQCAVCRRGDENLCAAPNCLGIHRHGGYADHILAPDPKYLIDLDGLDPAAMAPFACSG
                     LTAFSALKKVAAGLNETPLVIIGAGGLGLICLQILKALGGKGAIVADIDPIKREAAKK
                     NGALAAIDPRAPDALAQLTAANGGPVMAAIDFVGSEETSGLAFAALGRGGKLVMVGLF
                     GGAAGWSLPLIPIKAASILGSYVGNLAELRELVDLVRAGKVEPLPVTRCALHEAGQTL
                     TDLRAGRFIGRAVLTP"
     gene            complement(2055..4526)
                     /gene="clpA"
                     /locus_tag="VKU03_09055"
     CDS             complement(2055..4526)
                     /gene="clpA"
                     /locus_tag="VKU03_09055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009368 - endopeptidase Clp complex
                     [Evidence IEA];
                     GO_function: GO:0004176 - ATP-dependent peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0043335 - protein unfolding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit
                     ClpA"
                     /protein_id="HLJ71452.1"
                     /translation="MPSFSRALEKTLHRALSLASERHHEYATLEHLLLALIDDSDAAA
                     VMRACNVELEALRKTVIHHIDTELENIVVEGSEEAKPTAGFQRVVQRAVIHVQSSGRD
                     EVTGANVLVAIFAERETHAAYFLQEQDMTRYDAVNYISHGIAKRPGLSDATRTPRGAD
                     DDQESRESKEQKEGQDNKKKEAALEAYCVNLNKKARDGRIDPLIGREAEVQRTIQVLC
                     RRQKNNPLLVGEPGVGKTAIAEGLAHKIVKGMVPEVLAEATVFALDMGALLAGTRYRG
                     DFEERLKQVMKEIEQHKDAILFIDEIHTVIGAGATSGGAMDASNLLKPALAQGVLRCI
                     GSTTYKEYRQYFEKDRALVRRFQKIDVNEPSVADAIEILKGLKPYFEDFHKIRYTGEA
                     VKAAVELSARYIHDRKLPDKAIDVIDETGASQMLQPEAKRKKTIGVKEIEATIATMAR
                     IPPKAVSKDDAEVLQHLEDGLKRVVYGQDTAIAALASAIKLARAGLRDPEKPIGCYLF
                     SGPTGVGKTEVARQLAASLGVELTRFDMSEYMERHSVSRLIGAPPGYVGFDQGGLLTD
                     AIDQHPHCVLLLDEIEKAHPDLFNILLQIMDHGKLTDHSGKQIDFRNVILIMTTNAGA
                     QDLAKAAFGFHRSTREGDDKEAIDRLFAPEFRNRLDAIVPFGRLPTEVIAQVVDKFIL
                     QLEAQLADRNVTIELTDEARSWLIKNGYDEAMGARPMARVIQTAIKTPLADEVLFGRL
                     KNGGVVRVVVTNDENGLKKLGFVYPDGPARPRLERDILVAGRKRVEDEPEVRNARSRK
                     ARAGDGDAPEDDAPEAPPPEEEQAT"
     gene            complement(4537..4881)
                     /gene="clpS"
                     /locus_tag="VKU03_09060"
     CDS             complement(4537..4881)
                     /gene="clpS"
                     /locus_tag="VKU03_09060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease adapter ClpS"
                     /protein_id="HLJ71453.1"
                     /translation="MEPVAAREPRKGPEGPGSGAAVITRTRAQTKRPNMYRVLLLNDD
                     YTPMEFVIMVLQSYFNKNPEEATRIMLHVHHNGVGECGVFTYEVAETKVAQVMDHARK
                     NQHPLQCIMEKK"
     gene            complement(5232..5567)
                     /locus_tag="VKU03_09065"
     CDS             complement(5232..5567)
                     /locus_tag="VKU03_09065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phasin family protein"
                     /protein_id="HLJ71454.1"
                     /translation="MYATFEDFQKFGKQQFDAVAAAATSLTRGWQEIAAETTEFSKKA
                     IAANTDAFEKLLGAKTLDSAIQIQTEFAKSSYEDLVAETTKLGELYARLAKEAFKPVE
                     TAYAAVQPK"
     gene            6052..>7629
                     /locus_tag="VKU03_09070"
     CDS             6052..>7629
                     /locus_tag="VKU03_09070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase"
                     /protein_id="HLJ71455.1"
                     /translation="MAYAALTSPVDARGGWRYSGYAAHAPRHHYARAYRRSYRHHHYW
                     TYRAPAYSPPFSAMVVDENSGRTLYAVNQDALRHPASITKVMTLYLLFEQLEKGRITL
                     DTPIEISAHAASQAPSKLGLAPGDSLSTEDAIKAIVTRSANDIAVAIAEKIGGDEQTF
                     ADMMTRKARALGMTRTVYVNASGLPDDRQITTARDLTILGRAMEERFPRYFHYFSLHS
                     FEFAGETIGNHDHLLGRVDGVDGIKTGYTNASGFNLLTSLHRDGRSIVAVVLGGRSAG
                     ARDAMMEQLLYQHYAQASVARTAPMIAEASASEPIRVAGRRAEPAPLPPAREAASKEE
                     MEAESLAEGDDQEADQTPAPPARALAGRVAAAAPLPPVSLAERAEAPQPVKPVDTMRT
                     ASINAAESPGPAPALALRQAVAFKAEAPKPIEAAAQRLADPAALGWIKGPDGVVRSPD
                     PVPPVKPEAVLKQAMLAAAAAHPAAETRGAKPEESRAAQDGRLAKAEESRAAEETRIA
                     KSVAIQPVAHEGWKVQIA"
BASE COUNT         1275 a         2563 c         2408 g         1383 t
ORIGIN      
        1 ataaaggcaa cctaaaggtt gccattcgag atggacgacg atgacaccat tttccgcgcc
       61 ttggccgaag caagccgccg ccgattgctg gacctgctgc acgcccggaa cgggcgaacg
      121 ctcggcgaac tccgccggag cctcggcgcg agccgccaag ccgtggccaa gcatctcgcc
      181 gtcctcgagg gcgccgatct cgtcacgttt ctgcgcctcg gccgcgagaa gctgcatttc
      241 atcaatccgg cgccgatcaa cgcgatcgcc gagcgctgga tcaacaaatt cgaccgcggg
      301 cgcatcgccg cgctgtcgga tctcaagcgc aacttggaag gagaagaccc atgactagcc
      361 aagcccgaag ccagttcgtc tacgtcacct atatccgcac cacggccgac cgcctgtggt
      421 cggcgctgac cagcacggaa ttctcgcaaa aatattggct cggcgcccgg accgaagccg
      481 agtggcggaa aggcggcgcg tggcggatcg aatttcccga cggccgcctc gccgattccg
      541 gcgaaatcgt cgaattcgag ccggcgaagc ggctggcgat ccgctggcgc aacgaatggt
      601 cgcctgagct gaaggatgaa ggctggtcgc tctgcacgat ggagatcgag ccggccgccg
      661 gagaaagcgt caagctcacc gtcacgcacg cgatcgaacg cgagaattcg aagttcatcg
      721 gcgccgtgtc cggcggctgg ccgcagatcc tctccaacct caagtccctg ctcgagaacg
      781 gcgccgccat cttgccgccg aaatactgat tggcgacgcg tgcgtccgcg caccttgccg
      841 cgcggccaaa cgcgccgccc gggtccttcg tgcgttcttt gcgtccttcg tgttgagatt
      901 tcgacacgaa ggacgctcag gatcgacgaa ggacccgaga aagcgcttat ggcgtcagca
      961 ccgcccggcc gatgaagcgt ccggcgcgaa ggtccgtcag cgtttgaccg gcttcgtgca
     1021 gggcgcagcg ggtcaccggc agcggctcga ccttgccggc gcggacgaga tcgaccagct
     1081 cgcgcaactc ggcgagattg ccgacatagg agccgaggat cgaagccgcc ttgatcggga
     1141 tcagcggcag cgaccagccg gcggcgccgc cgaaaaggcc gaccatgacc agcttgccgc
     1201 cgcgcccgag cgcggcgaag gcgaggccgg acgtctcttc gctgccgacg aaatcgatcg
     1261 ccgccatcac cgggccgcca ttggcggcgg tcaattgcgc cagcgcgtcg ggcgcgcgcg
     1321 gatcgatcgc ggcgagcgcg ccgttctttt tcgccgcctc gcgcttaatg ggatcgatgt
     1381 cggcgacgat cgcgcccttg ccgccgagcg ccttgaggat ttgcaggcag atcaagccaa
     1441 gcccgccggc gccgatgatc accagcggcg tctcgttcag cccggccgcc accttcttca
     1501 gcgcgctgaa agcggtgagg cccgaacagg cgaagggcgc catcgccgcc ggatcgagcc
     1561 cgtcgagatc gatgaggtat ttcggatcgg gcgcaagaat atggtcggca tagccgccat
     1621 gccggtggat gccgaggcag tttggcgcgg cgcaaagatt ttcatcgccg cgccggcaga
     1681 ccgcgcactg gccgcagccg atccagggat agacgaggta cgtcctgccg atcgcgacgc
     1741 ccttggcgtc gggcccgagc gcgacgacgc ggccggcggt ttcgtggccc atcgtcagcg
     1801 gcagattgat accgcggtcc ttcagcgaca gtcttttgcc gcggccgagg tcgtagccgc
     1861 cttcccagat atgcagatcg ctatggcaaa cgccggccgc gacgacttcg agcagcgctt
     1921 ccgcgccttg cggcttcggc gtcggcgcct cggcttcgac gagcggcgcg ccgaagtcga
     1981 cgacttggta gcttctcatt gcgcccctcc ccgcgcgccg cgtcatacgc gcttacgcgc
     2041 gaatgacgag ccctttacgt cgcctgctct tcttccggcg gcggcgcttc gggcgcgtcg
     2101 tcctcgggcg cgtcgccgtc gccggcgcgc gccttgcggc tgcgggcgtt gcgcacttcc
     2161 ggctcgtcct cgacgcgctt gcggccggcg acgaggatgt cgcgctcgag ccgcggccgc
     2221 gccgggccgt ccggatagac gaagccgagc ttcttcaagc cgttctcgtc attggtgacg
     2281 acgacgcgca ccacgccgcc attcttcaag cggccgaaca ggacctcgtc ggcgagcggc
     2341 gtcttgatcg cggtctggat gacgcgcgcc atcggccgcg cccccatcgc ctcgtcatag
     2401 ccgttcttga tcagccagga gcgggcctcg tcggtcagct cgatcgtcac gttgcggtcg
     2461 gcgagctgcg cctccagctg caggatgaac ttgtcgacga cctgggcgat gacctcggtc
     2521 ggcaggcggc cgaacggcac gatcgcgtcg agccgattgc ggaattccgg cgcgaacagc
     2581 cggtcgatcg cctccttgtc gtcgccttcc cgcgtcgagc gatggaagcc gaacgccgcc
     2641 ttggcgaggt cctgcgctcc ggcattggtc gtcatgatca agatcacatt acggaaatcg
     2701 atctgcttgc cggaatggtc ggtgagcttg ccgtgatcca tgatctgcaa cagaatattg
     2761 aacagatcgg gatgcgcttt ctcgatctcg tcgagcagca gcacgcaatg cggatgctgg
     2821 tcgatcgcgt cggtgagcag gccgccctgg tcgaagccga catagccggg cggcgcgccg
     2881 atcagccgcg agaccgagtg gcgctccata tattccgaca tgtcgaagcg ggtaagctcg
     2941 acaccgaggc tggcggcgag ctggcgggcg acttccgtct tgccgacgcc ggtcgggcct
     3001 gagaacagat agcagccgat cggcttttcc gggtcgcgca aaccggcccg ggcgagcttg
     3061 atcgccgaag ccaaagccgc gatcgccgtg tcctggccat agacgacgcg cttcaagccg
     3121 tcttcgagat gctgcagcac ctcggcgtcg tccttggaga cggccttggg cggaatgcgc
     3181 gccatcgtgg cgatcgtcgc ctcgatttcc ttgacgccga tcgtcttctt gcgcttggcc
     3241 tcaggctgca gcatttgcga ggcgccggtc tcgtcgatga cgtcgatcgc cttgtccggc
     3301 aatttgcggt cgtgaatgta gcgcgccgac agttcgaccg ccgccttgac cgcttcgccg
     3361 gtgtaacgga ttttgtggaa gtcctcgaaa tagggcttga ggcctttgag gatttcgatc
     3421 gcgtcggcga cgctcggctc gttcacgtcg atcttctgga agcgccggac gagagcgcgg
     3481 tccttctcga aatattgccg gtactccttg tacgtcgtcg agccgatgca gcgcagcacg
     3541 ccctgcgcca gcgccggctt caagagattc gaggcgtcca tggccccgcc cgaggtggcc
     3601 cccgcgccga tcacggtgtg aatctcgtcg atgaacaaga tcgcgtcctt gtgctgttcg
     3661 atctccttca tcacctgctt caggcgctcc tcgaaatcgc cgcgatagcg cgtgccggcg
     3721 agcagcgcgc ccatatcgag ggcgaagacc gtcgcctcgg cgagcacttc cggcaccatt
     3781 cccttgacga tcttatgcgc caagccctcg gcgatcgccg tcttgccgac gccgggctcg
     3841 cccaccagaa gcggattgtt cttctggcgg cggcacagaa cctggatcgt gcgctgcact
     3901 tcggcctcgc ggccgatcag cggatcgatc cgcccgtccc tcgccttctt gttaaggttg
     3961 acgcagtaag cttcgagcgc agcttccttt ttcttattgt cctgcccttc cttctgctct
     4021 ttcgactcgc gcgattcctg gtcgtcgtcg gcgccgcgcg gcgtgcgcgt cgcatccgac
     4081 aggccgggcc gcttggcgat gccgtggctg atgtaattga ccgcgtcgta gcgcgtcatg
     4141 tcctgctctt gcaggaaata ggcggcatga gtctcgcgct ccgcgaaaat cgcgaccagc
     4201 acgttggcgc cggtcacctc gtcgcgtccc gaggactgga cgtgaatgac ggcgcgctgc
     4261 acgacgcgtt ggaatccagc ggtcggcttg gcttcttcgc tgccctcgac gacgatgttt
     4321 tcgagttccg tatcaatgtg atggatgacg gtcttgcgga gcgcttcgag ctccacattg
     4381 cacgcccgca tgaccgctgc ggcgtcgctg tcgtcgatca gcgcgagcag cagatgttca
     4441 agcgtcgcat attcgtgatg acgctcgctg gcgaggctga gggcccggtg gagggtcttt
     4501 tcgagggcgc gcgaaaagga tggcatggag cgcctttcac ttcttttcca tgatgcattg
     4561 cagcggatgt tggttcttcc gggcgtggtc catgacctgc gccactttcg tctcggcgac
     4621 ctcgtaggtg aagacgccgc attcccccac gccgttatgg tggacgtgga gcatgattct
     4681 cgtcgcctcc tccgggttct tgttgaagta ggattgcaag accatgatga cgaactccat
     4741 cggcgtgtag tcgtcattga gcagcaggac gcgatacatg ttcggccgct tggtttgggc
     4801 gcgcgtgcgg gtgatcaccg ccgcgcccga gccgggacct tccggaccct ttcgaggctc
     4861 cctagccgcc acaggttcta tccgcccgtt cgccgccggc gtccgccccg cgccgagcgg
     4921 agcaaacaac cagcgcgcca ccatcgccat ccgcgctctc aaacgtccgg cgcccacaat
     4981 ggtttcgcca gccactttcg acctctcgac ccaaccgctt cgatccaaac gcgacgcttg
     5041 agaatgcgtc gctcgcattc gaatgatcgg tctaatttaa ggcccttgca aggctcacgc
     5101 caggggtccg gcggcggccg ccgatcaaat aagaagaggc ccggcatagg ccgggcctct
     5161 tgttacgcaa cgctcgaacg caaatcgcca gcgttcgaaa ctcgaaaaca aacttgccga
     5221 aaagccggcg cttacttcgg ctgaaccgca gcgtaggccg tctcgaccgg cttgaacgcc
     5281 tccttggcga gacgcgcata gagctcgccg agcttggtcg tctcggcgac gaggtcctca
     5341 tagctcgact tggcgaactc ggtctgaatc tggatcgcgc tgtcgagcgt cttggcgccg
     5401 agcagcttct cgaaggcgtc ggtgttggcg gcgatcgcct tcttcgagaa ttccgtggtc
     5461 tcggcggcga tctcctgcca accgcgagtg agcgaggtcg ccgcagcggc caccgcgtcg
     5521 aattgctgtt tgccgaattt ctggaaatct tcaaacgtcg cgtacatttc gtctccgcct
     5581 ttccatcaca tgtcgccggc cgctcgggcc tggcccgccg atcgctcagg gaccgctgaa
     5641 agcgaatatg tgcggcgcac aataaatgtc aagattattt tgcgacgcac catcaaaatt
     5701 cccgcggccg tcgcgatcat gacccatttg caaggcaatt cgatgaagtc gcgggcggcc
     5761 gaagagcgaa ggcgaagcgt cgagccgatt ggctcggata tttaccttgt tgagcttttc
     5821 tctttgacct catgggcttg cggccaaaaa gcgcggcttg gcgctttcgc attaacgcga
     5881 gagtaaccgc gtcgccatag gttttacgca tgtgtggtaa gcgcaacggt cgccgcgagg
     5941 ccaatcgcgc ccgtttcgcc tagatgagtt ggggcagtta tggcgttagg tctgggggcg
     6001 agagccggcg gccgtcttac gcgtcttatt tccggattgt gcgtatcgct attggcttac
     6061 gccgcgctga cctcgccggt cgacgcgcgc ggcggctggc gttattccgg ctatgccgct
     6121 catgcgccgc gccaccatta cgcccgcgcc tatcggcgct cctaccgtca tcatcactat
     6181 tggacctatc gcgcgccggc ctattcgccg ccgttctcgg cgatggtcgt cgacgagaat
     6241 tccgggcgca ctctctacgc ggtcaaccag gatgcgctgc gccatccggc ctcgatcacc
     6301 aaggtgatga ccctctacct gctcttcgag caattggaga agggccgcat aacgctcgac
     6361 acgccgattg aaatctcggc gcatgcggcg agccaggcgc cgtccaagct cggcctcgcc
     6421 cccggcgact cgctctcgac cgaagatgcg atcaaggcga tcgtcacccg ctccgccaac
     6481 gacatcgcgg tcgccatcgc cgagaagatc ggcggcgacg agcagacttt cgccgacatg
     6541 atgacgcgca aagcgcgcgc gctcggcatg acgcgcaccg tctatgtcaa cgcttccggc
     6601 ctgcctgacg atcgccagat cacgacggcg cgcgacttga cgattctcgg ccgggcgatg
     6661 gaagagcgct ttccgcgcta ctttcattat ttttctctcc attccttcga attcgccggc
     6721 gagacgatcg gcaaccacga tcatctgctc ggccgcgtcg acggggtcga cggcatcaag
     6781 acgggttaca ccaacgcctc cggcttcaat ctcctgacct cgctgcatcg cgacggccgc
     6841 tcgatcgtcg ctgtcgtgct gggcggacgc tcggccggcg cgcgcgacgc catgatggag
     6901 caactccttt atcaacacta cgcgcaagcc agcgtcgcgc gcaccgcgcc gatgatcgcc
     6961 gaagccagcg cgtccgagcc gatccgcgtc gccgggcgcc gcgccgagcc ggcgccgctc
     7021 ccgccggccc gtgaagcggc cagcaaggag gagatggagg ccgaatcgct cgccgaaggc
     7081 gacgaccagg aagccgatca gacgccggcc ccgccggccc gcgctttggc cggccgcgtc
     7141 gcggctgcgg cgccgctgcc gccggtcagc ctcgccgaac gcgccgaggc gccgcagccc
     7201 gtaaagccgg tcgatacgat gaggaccgct tcgatcaacg ccgcggagtc gccgggcccg
     7261 gcgccggcgc tcgccctccg tcaagccgtc gcgtttaagg ccgaggcgcc gaagccgatc
     7321 gaagccgccg cgcagcgcct cgccgacccc gccgcgctcg gctggatcaa gggccccgac
     7381 ggcgtcgtcc gcagtcccga tccggtcccg ccggtcaagc cggaagccgt gctcaagcag
     7441 gcgatgctcg ccgcggccgc ggctcacccg gccgccgaga cgcgcggcgc caagccggaa
     7501 gaaagccgcg ccgcgcaaga tgggcgcctc gccaaagccg aggaaagccg cgccgccgag
     7561 gagacccgca tcgcgaaatc cgtagcgatc cagccggtcg cgcacgaggg ctggaaagta
     7621 cagatcgcg
//
LOCUS       DATGJW010000297         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1194725, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000297 DATGJW010000000
VERSION     DATGJW010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194725"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..821)
                     /gene="puuE"
                     /locus_tag="VKU03_09075"
     CDS             complement(<1..821)
                     /gene="puuE"
                     /locus_tag="VKU03_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA];
                     GO_process: GO:0000256 - allantoin catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="allantoinase PuuE"
                     /protein_id="HLJ71456.1"
                     /translation="MTDDAYPRDMVGYGRTPPYADWPNGARIALQFVVNYEEGGENNI
                     LHGDAASEAFLSEIVGAAPWPGMRHMNMESIYEYGSRAGFWRLHRLFTERDMPVTVYA
                     VALAMRRNPAAIAAMNEAGWEIATHGYKWIDYRDTPIEVERQHIAEAIKIHTEVAGER
                     PLGFYQGRSSINTIPLGMEEGGFLYCADAYADDLPYWIEGPKSPQLIIPYTLDSNDMR
                     FATAGGFNSGDQFYSYLKDSFDTLYAEGATAPKMLSIGLHCRLVGRPGRAASLAR"
     gene            1011..1808
                     /locus_tag="VKU03_09080"
     CDS             1011..1808
                     /locus_tag="VKU03_09080"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLJ71457.1"
                     /translation="MDEIALAIEAPLARIRLSRPERRNAVSLAMWRALPELCWRIEAA
                     KDALVVIVEGVGDHFSVGADINEFGAVYRNAETTRDYLHAIQDGLNALIALDKPTIAA
                     LRGNAIGGGFALGLCCDLRFGADNVHLAITASKLGLLFGFAETRRLVDLVGPARAKDI
                     LYSARRIDGAEALAIGLIDRLTPADRLSGAVEAYARDLAGLSQQSIRGAKFAVEAITR
                     GLTTETQAFRRLIEDAARGEDSLEGRRAFAEKRAPNFKFRGRVENPD"
     gene            complement(1818..2309)
                     /locus_tag="VKU03_09085"
     CDS             complement(1818..2309)
                     /locus_tag="VKU03_09085"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266836.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLJ71458.1"
                     /translation="MNERAVAAQFSQTSPDRATTSGLDFIRAIKDGAHPPPSMATLIG
                     FDLVEAEEGRVVFSGEPTERHLNPMGVVHGGYAATLLDSCMTCAIQSALKPGFAATTL
                     DIAVHFTRAAKPRSGALRAEGKTIHVGRQFGTAEGRLTDSQGRVIAHATTSCLIFPMR
                     PPA"
     gene            complement(2466..3599)
                     /locus_tag="VKU03_09090"
     CDS             complement(2466..3599)
                     /locus_tag="VKU03_09090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856779.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ71459.1"
                     /translation="MQTFSLGVVGCGAISSVYLRNVPLFKGLTVSACADVKPEAAAKQ
                     AKAFGVPALGVEDLLASPDVDIVVNLTVPNAHYEVSMAALKAGKHLFTEKPLAARAAL
                     GRKLVEAAKKRGLAIASAPDTFLGAAGRKARALIDHGAIGRPVAGAAFVLGHGMEHWH
                     PNPDFFYKPGGGPVLDLGPYYITALINLLGPIARVQALTSIGEAKRLITAPGPNRGKT
                     IKVETPTTALALLEFANGVHLVAGMSWDVWRHGAAPIEIHGTEGSLRLPDPNFFGGVV
                     EVSKRGGEWAAHDTSADPFGVLNQPAAEPKVANYRVLGIADLAAAIREKRPPRAGGDL
                     ALHALEAMEAILRAGAERRAIRLPPADVRPAVLSEKEARALLA"
     gene            complement(3663..>4149)
                     /locus_tag="VKU03_09095"
     CDS             complement(3663..>4149)
                     /locus_tag="VKU03_09095"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLJ71460.1"
                     /translation="FLPPPQRPNDAEGWRAFGARLAAIAAKFDAEGIGFGWHNHDFEM
                     RALPDGSYPIDRLLSASDKIKWQADIGWIARAGQDPLRWIARYAGRIEALHVKDLAKP
                     GENEKEEGWADPGAGTLDLKAIVSAGIAAGAKFLVVEHDAPSDYERFARNAIAAARGW
                     A"
BASE COUNT          712 a         1361 c         1382 g          694 t
ORIGIN      
        1 aatcgcgcca gcgaagcggc gcggccgggc cggccgacca ggcggcaatg aaggccgatc
       61 gacagcattt tcggcgcggt cgcgccttcc gcgtagagcg tgtcgaagga gtccttgagg
      121 tacgaataga actggtcccc ggaattgaaa ccgccggccg tcgcaaaacg catatcgttc
      181 gaatcgagcg tatagggaat gatgagctgc gggcttttcg gcccctcgat ccaataggga
      241 aggtcgtcgg cataggcgtc ggcgcaatag agaaagccgc cctcctccat gccgagcgga
      301 atcgtgttga tcgacgagcg gccctggtaa aagccgagcg gcctttcgcc ggcgacctcg
      361 gtgtggattt tgatcgcctc ggcgatgtgc tggcgctcga cctcgatcgg cgtgtcgcga
      421 tagtcgatcc atttgtagcc gtgggtggcg atctcccaac cggcttcgtt catcgcggcg
      481 atcgcggccg gattgcgccg catcgccagg gccaccgcat agacggtgac cggcatgtcg
      541 cgctcggtga acaggcgatg caagcgccag aaaccggcgc gcgagccata ttcgtagatc
      601 gactccatgt tcatgtggcg catgcccggc cagggcgcgg cgccgacgat ttccgacagg
      661 aacgcctccg aggcggcgtc gccatgcaag atgttgttct cgccgccctc ttcgtagttg
      721 accacgaatt gcagcgcgat ccgcgcgccg ttcggccaat cggcgtaagg cggggtgcgg
      781 ccatagccga ccatgtcgcg cgggtaggcg tcgtctgtca tggaaaagcc cctcgggaag
      841 tccctgtcag gataaacgta aagcagccgc tctcttcttc caaacttttg gaaaggtgct
      901 tccgccaggc tcgaagacgc gccgccctcg cccgcttaac ccttccttcg ccaaatcgcc
      961 ggcaggatag cgcaggggca taggcgaagc ttgagcgcgg agggggagca atggatgaaa
     1021 tcgccttggc gatcgaagcg ccgctggcgc ggatcaggct cagccggccg gagcgccgca
     1081 atgcggtctc gcttgcgatg tggcgcgctc tgccggaact gtgctggcgc atcgaagcgg
     1141 ccaaggacgc gctggtggtg atcgtcgagg gcgtcggcga tcatttcagc gtcggcgccg
     1201 acatcaacga attcggcgcg gtctatcgca acgccgagac gacccgcgat tatctccacg
     1261 ccattcagga tggattgaac gcgctgatcg cgctcgacaa gccgaccatc gccgccttgc
     1321 gcggcaatgc gatcggcggc ggcttcgccc ttggcctttg ctgcgatctg cgctttggcg
     1381 ccgacaatgt ccatctcgcg atcaccgcgt ccaagctcgg cctgctgttc ggcttcgccg
     1441 agacgcgccg cctggtcgat ctcgtcggtc cggcgcgcgc caaggacatt ctctacagcg
     1501 cgcggcgcat cgacggcgcg gaagcgctgg cgatcggcct gatcgatagg ctgacgcctg
     1561 cggatcgttt gagcggagcg gtggaggcct acgcgcgcga tctcgccggg ctgagccagc
     1621 aatccatccg cggcgcgaaa ttcgcggtcg aagcgatcac gcgcggcctg accaccgaga
     1681 cgcaggcctt ccgccgtctg atcgaagacg ccgcgcgtgg cgaagacagt ctcgaaggcc
     1741 gccgcgcttt cgccgagaag cgcgcgccga atttcaaatt ccgcggacgc gtcgaaaatc
     1801 ccgactgatc cttcggctca agccggcggc cgcatcggga agatcaggca ggaggtggtc
     1861 gcgtgagcga tcacccggcc ctggctgtcg gtgaggcgcc cttcggcggt gccgaattgc
     1921 cggccgacat gaatcgtctt gccttccgcc cgcaaagcgc cggatcgcgg cttcgccgcc
     1981 cgggtgaaat gcaccgcgat gtcgagcgtc gtcgcggcga agccgggctt cagcgccgat
     2041 tggatcgcgc aagtcatgca ggaatcgagc aaggtcgctg cgtagccgcc atgcacgacg
     2101 cccatcggat tgagatggcg ctcggtcggc tctccggaaa agacgacccg gccctcctcg
     2161 gcttcgacga ggtcgaaacc gatcagcgtc gccatggacg gcggcggatg cgcgccgtcc
     2221 ttgatcgcgc ggatgaaatc gaggccgctc gtcgtggccc gatcggggga cgtttggctg
     2281 aattgcgcag cgaccgcccg ttcgttcatg cccagcctcc tcgcttggcg caagacaaac
     2341 cgaatatagt ttacatataa actaagcacg ccgacggctg agaggcaatg gcgacggcgc
     2401 gccggcatgc ctggcttgcg gagacatcct ggcttcgcgc cgcgccagga agggtcgaca
     2461 gccggtcacg ccagcagcgc gcgcgcctct ttttcgctga gaaccgccgg cctgacatcg
     2521 gccggcggca ggcggatcgc gcggcgctcg gcgccggcgc gcaagatcgc ctccatggct
     2581 tcgagcgcat gcagcgcgag atcgccgccc gcccgcggcg gacgcttctc gcggattgcg
     2641 gcggcgagat cggcaatgcc gagcacgcga tagttcgcga ctttcggctc ggccgccggc
     2701 tggttcaata cgccgaacgg atcggccgac gtatcatgcg ccgcccattc gccgccgcgc
     2761 ttggagactt cgacgacgcc gccgaagaaa ttcggatcgg gcaggcgcag ggagccctcc
     2821 gtaccgtgaa tttcgatcgg cgcggcgccg tggcgccaaa cgtcccagct catcccggcg
     2881 acgagatgga cgccgttcgc aaattcaagc agcgcgagcg ccgtggtcgg cgtctcgacc
     2941 ttgatcgtct tgccgcgatt cgggccgggc gcggtgatca gccgcttcgc ctcgccgatc
     3001 gacgtcagcg cctggacgcg cgcgatcggg ccgagcaagt tgatcagcgc ggtgatgtag
     3061 tacggtccga gatcgagcac cggcccgccg cccggcttat agaaaaagtc gggattggga
     3121 tgccaatgct ccatgccgtg gccgagcacg aaagcggcgc ccgcaaccgg ccggccgatc
     3181 gcgccgtgat cgatcagcgc ccgcgccttg cggcccgccg cgccgagaaa agtgtccggc
     3241 gccgaggcga tggcgaggcc acgcttcttc gcggcctcga cgagcttgcg gcccaaagcg
     3301 gcgcgcgcgg cgagcggctt ctcggtgaag agatgcttgc cggccttcag cgccgccatc
     3361 gaaacctcgt aatgggcgtt gggcacggtg agattgacga cgatgtcgac atccggcgaa
     3421 gcaagcaggt cctccacgcc gagcgccgga acgccgaaag ccttggcctg cttggccgcg
     3481 gcttcgggct tgacgtcggc gcaggccgag actgtcaggc ctttgaagag cggaacgttg
     3541 cgcaaataga cggacgagat ggcgccgcaa ccgacaacgc cgagagagaa ggtttgcatg
     3601 cgcgctccaa gaaacaatgg gccgtgagct tatcctcgcc gtcttcgact ggggaggatt
     3661 tgtcacgccc atcctcgcgc cgcggcgatc gcgttgcgcg cgaagcgctc gtaatcgctc
     3721 ggcgcgtcgt gctcgacgac gaggaatttc gcgcccgccg caattcccgc cgagacgatc
     3781 gccttcaagt caagcgttcc ggcgccggga tcggcccagc cttcctcttt ctcgttctcg
     3841 cccggcttgg cgaggtcctt cacatgcagc gcctcgatcc ggccggcgta acgcgcgatc
     3901 cagcgcaacg ggtcttggcc ggcccgcgcg atccagccga tgtcggcttg ccatttgatc
     3961 ttgtcgctcg ccgacaacag ccgatcgatc gggtacgagc cgtccggcag cgcgcgcatc
     4021 tcgaaatcgt gattgtgcca gccgaagccg attccctcgg cgtcgaattt cgccgcgatc
     4081 gcggcgagac gggcgccgaa agcgcgccat ccttccgcat cgttcgggcg ctgcggcggc
     4141 ggcaggaac
//
LOCUS       DATGJW010000298         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1094559, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000298 DATGJW010000000
VERSION     DATGJW010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1094559"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            211..1020
                     /locus_tag="VKU03_09100"
     CDS             211..1020
                     /locus_tag="VKU03_09100"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HLJ71461.1"
                     /translation="MIIPCDEAIEALRKRGLSTLPPIAIVLGSGLGSLADEVENAISV
                     PYEDVPGFPVPTVSGHAGRLVVGELEGERVALFQGRGHYYERGDARAMAVAIATFKQL
                     GGETLLLTNAAGGLRVEWRPPALVAITDHINYAGVNPLIGLHTDDRFVPLTKAYDPAL
                     RARLMHAAGEAGVEMHEGVYMWFSGPSFETPAEIRAAKTLGADLVGMSTVPEVILARL
                     FGLKCVAVSLVTNFGAGLAGGDPSHHETKELALLGSANFKKLLRAFLRETP"
     gene            1217..2545
                     /gene="deoA"
                     /locus_tag="VKU03_09105"
     CDS             1217..2545
                     /gene="deoA"
                     /locus_tag="VKU03_09105"
                     /EC_number="2.4.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009032 - thymidine phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine phosphorylase"
                     /protein_id="HLJ71462.1"
                     /translation="MILPQEIIRKKRDGLALDASEIEAFIAGLTSGEVTEGQAAAFAM
                     AVFFKGMTLDERVALTRAMMASGTVLDWRSANLPGPILDKHSTGGVGDNVSLMLAPML
                     AACGAFTPMISGRGLGHTGGTLDKLDSIPGYVAQPDIALFRRTVAEAGCAIIGQTADL
                     APADKRLYAIRDVTATVESIPLITASILSKKLAAGLQGLVMDVKAGSGAFMATLDGAR
                     DLAKSLAHVATGAGLPTDALITDMNEPLASAAGNAVEVLNAVEYLTGARRDARLHAVT
                     KALGGQLLALGGLARDAAEGEAAMEKSLASGAAAERFERMVAMLGGPSGFLDKAPSLL
                     PKAPVLVAAPPARRGVVVEIDVRAVGMAVVAMGGGRARAADTIDPAVGFTALAGLGAE
                     VGPDAPLALVHARDDAQAKAAGAALAAAYGIGDAPPATRAPVIERIAGKS"
     gene            2542..3489
                     /locus_tag="VKU03_09110"
     CDS             2542..3489
                     /locus_tag="VKU03_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494567.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate/hydroxypyruvate reductase A"
                     /protein_id="HLJ71463.1"
                     /translation="MTILLAIEPASLAAVWERRLRILLPDRKIIPSGALVDRGAVAYA
                     LSWRHHAGTLADLPHLKAIFSLGAGVDHMFSDPLLPEAPIVRFVDDDLTNRMSEWVVL
                     HALLHLRQQKLYDRQQAQRHWEEDLNQPSARDVRIGLMGLGALGVDAARKLKIIGFDV
                     AGWSRTPKAVEGLLCFAGPEGLSAMLARTDMLVALLPLTPETRGILNASLFAKLAPDG
                     RLGGPILINAGRGGLQVEKDIVEALDAGVLKAASLDVFEAEPLPETSPLWRHPRVYVS
                     PHSAALSEPSAVAAAIAKQIEAFERGEPLKNVVDRARQY"
     gene            3545..>3768
                     /locus_tag="VKU03_09115"
     CDS             3545..>3768
                     /locus_tag="VKU03_09115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter"
                     /protein_id="HLJ71464.1"
                     /translation="MQRYIAQRLLYSVATLFAIITIAFFLIRVAPGGPFNLERPLDPR
                     IMENLRRLYRLDEPLWRQYLDYLSGLARGD"
BASE COUNT          622 a         1269 c         1183 g          694 t
ORIGIN      
        1 actggatcaa ttgctgcccg aatcttttgg cccagaacat ttaaagtgag gcgcaatccg
       61 tcccagacga cacgcagcgc ggtagagggg gtgaggattt gcttcaaccg ccgtaccctt
      121 ccaccatgct tcgcatggcc ccctcccccg tcttcgacag gaacgaggcg cgcgcttttt
      181 cagtcctgag ctccatggtg aaggcaaatc atgatcatcc cctgcgacga agcgatcgaa
      241 gccttacgca aacgcggcct ctccacgctt ccgccgatcg ccattgtcct cggctccggc
      301 ctcggctcgc ttgctgacga ggttgaaaac gcgatctccg taccgtacga agacgtgccc
      361 ggtttcccgg tcccgactgt ttcgggccat gccggacgat tggtcgtcgg cgagctcgag
      421 ggcgagcgtg ttgctctatt ccaggggcgc ggccattatt acgagcgcgg cgatgcgcgc
      481 gcgatggcgg tcgcgatcgc gacattcaag cagctcggcg gcgagacttt gctactgacc
      541 aatgcggccg gcggcctgcg tgtcgaatgg cgcccgccgg cgctggtcgc gatcaccgat
      601 cacatcaact acgccggcgt caatcccttg atcggattgc atacggacga tcgtttcgtg
      661 ccattgacca aagcctatga tccggcgctg cgcgcccgac tgatgcatgc ggcgggcgaa
      721 gccggcgtcg agatgcacga gggcgtctat atgtggttct ccggcccgtc cttcgagacg
      781 ccagcggaaa ttcgcgccgc gaaaaccctc ggcgccgatc tcgtcggcat gtcgaccgtc
      841 ccggaagtca ttctcgcccg gctcttcggc ctcaaatgcg tcgccgtctc cctggtgacc
      901 aatttcggcg caggtctcgc cggcggcgat ccgagccatc acgagacgaa agaactcgcg
      961 ctattggggt cggcgaattt caagaagttg ctgcgggcgt ttttgaggga aacgccatga
     1021 gttgtcgcga ccagcaatcg cttaaaacta ttgcgatcgg ggccgcggct tcccctcacg
     1081 cgcgcgggag aagggattct ctggcgccgc agaactacgc ggcaggcgcg gcgcgcgccc
     1141 tctcccgctt gcggaagagg attggggtga gggtaagccg ccccgacgac cgaagctcct
     1201 ctcatagacg acactcatga tcctccccca agaaatcatc cgcaaaaagc gcgacggcct
     1261 cgccctcgac gcctcggaaa tcgaagcctt catcgcgggc ctcacctcgg gcgaggtgac
     1321 cgaaggccag gccgccgctt tcgccatggc ggtctttttc aaaggcatga cgttggatga
     1381 gcgcgtcgcc ctgactcgcg cgatgatggc gtcggggaca gtgctcgact ggcgcagcgc
     1441 gaatcttccg ggtcccatcc tcgataaaca ttcgaccggc ggcgtcggcg acaatgtcag
     1501 cttgatgctg gcgccgatgc tggccgcctg cggcgccttc acgccgatga tttccggccg
     1561 cggtctcggc cataccggcg gcacgctcga taagcttgat tctatccccg gctatgtcgc
     1621 gcagcccgat atcgcgctgt tccggcgcac cgtcgccgaa gccggctgcg cgatcatcgg
     1681 ccagactgcc gatctcgccc ccgccgacaa gcgcctctac gccatccgcg acgtgacggc
     1741 gacggtcgaa tccatcccgc tcatcaccgc ttcgatcctg tcgaagaagc ttgccgccgg
     1801 gttgcaaggc ctcgtcatgg acgtgaaggc cggctccggc gccttcatgg cgacgctgga
     1861 cggggcgcgg gatttggcca agagcctcgc tcatgtcgcg accggcgcag gcctgccgac
     1921 cgacgcgctg atcaccgata tgaacgagcc gctcgccagc gcggccggca atgcggtcga
     1981 ggtgctgaac gccgtcgaat atctcaccgg cgcaagacgc gacgcacgcc ttcacgccgt
     2041 gaccaaggcg ctgggcggtc agttgctcgc cttgggcggc ttggcgcgcg atgccgccga
     2101 aggcgaagcg gcgatggaga agagcctcgc gagcggcgcc gcggccgagc gtttcgagcg
     2161 catggtggcg atgctcggcg gcccgagcgg ttttctcgac aaggcgccaa gtctattgcc
     2221 gaaggcgccg gtccttgtcg ccgcgccgcc ggcgcggcgc ggcgtcgttg tcgaaatcga
     2281 cgtacgcgcg gtcggcatgg cggtcgtcgc catgggcggc ggtcgcgccc gggccgccga
     2341 tacgatcgat ccggccgtcg gcttcacggc gcttgccggg ctcggcgcgg aggtaggccc
     2401 cgatgcgccg cttgctcttg tccacgcgcg ggacgacgca caggcgaaag cggccggcgc
     2461 cgcccttgcg gcggcctatg gcatcggcga tgcgccgccc gcgacccgcg cgcccgtcat
     2521 cgaacgcatc gctggcaagt cgtgacgatc cttctggcga tcgaaccggc gagcctggcc
     2581 gccgtctggg aaaggcggct gcgcattctt ctgcccgatc ggaagatcat cccgtcagga
     2641 gccctggtcg atcgcggcgc ggtcgcttac gcgctctcct ggcgccatca cgccggaacg
     2701 ctcgcggatt tgcctcatct caaagcgatc ttttctctcg gcgccggcgt cgatcatatg
     2761 ttcagcgatc ccttgctgcc cgaagcgccg atcgttcgtt tcgtcgacga cgatctcacc
     2821 aaccggatga gcgaatgggt cgttctgcat gcgctgctcc atctgcgtca gcaaaagctc
     2881 tacgaccgac aacaagccca gcgtcattgg gaggaggatc tcaaccagcc gagcgccaga
     2941 gatgtgcgca tcggcctgat gggcctcggc gcgcttgggg tcgacgcggc gcggaagctg
     3001 aagatcatcg gcttcgatgt cgccggctgg agccgcacgc cgaaagccgt tgaaggtctt
     3061 ctctgtttcg ccggccccga aggcctcagc gccatgttgg cgcggaccga catgctcgtc
     3121 gcgcttttgc cgctgacgcc cgagacgcgc ggcattctga acgcgtcgct tttcgccaaa
     3181 ctggcgccgg acggcagact tggcggaccg atcctgatca acgcgggtcg cggcggcctc
     3241 caagtcgaaa aggacatcgt cgaggcgctc gacgccggcg tgttaaaagc cgcttcgttg
     3301 gatgtcttcg aggccgagcc tttgccggaa acctcgccgc tgtggcgcca tccgcgcgtc
     3361 tatgtcagcc cgcacagcgc cgccctctcc gagccaagcg cggtcgccgc cgccatcgcc
     3421 aagcagatcg aagccttcga acgtggcgag ccgctgaaaa atgtggtcga tcgcgcccgg
     3481 cagtactaga ctctctctct ccctccttcg tcggaagacg aatcgcaggc tggaaggcgc
     3541 gccattgcaa cgctacatcg ctcagcgctt gctttattcc gtggcgacgc tctttgcgat
     3601 catcacgatc gctttctttc tgatccgcgt tgcgccaggc ggcccgttca acctcgaaag
     3661 gcctctcgat ccgcgcatca tggagaattt gcgccggctc taccggctcg atgagccgct
     3721 ctggcggcaa tatctcgatt atctctccgg cctcgcgcgc ggcgattt
//
LOCUS       DATGJW010000299         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_989441, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000299 DATGJW010000000
VERSION     DATGJW010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989441"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..362
                     /locus_tag="VKU03_09120"
     CDS             <1..362
                     /locus_tag="VKU03_09120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLJ71465.1"
                     /translation="RDSFEVTIVPGVSSICGAWGAAGEPMTWGDDALVVLPATLTLDE
                     LTRRLRLADAAVIMKIGRNFARAKQALVAAGMAERAIYVERATMAGEAVTPLCQKRDD
                     AAPYFSLILAPGRGRRP"
     gene            359..1117
                     /gene="cobJ"
                     /locus_tag="VKU03_09125"
     CDS             359..1117
                     /gene="cobJ"
                     /locus_tag="VKU03_09125"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008531528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HLJ71466.1"
                     /translation="MTGRVAIVGLGPGSAEWMTPEATAAVAAASDIVGYGPYLDRLRL
                     RPDQRAHPSDNRHELERARFALTLAAEGRAVAVVSGGDPGVFAMAAAVFEAIEEAPEW
                     RRFDIRVAPGVTAMQAAAARLGAPLGHDFCAISLSDNLKPWAIVERRLRAAAEGDFVI
                     ALYNPASRARPRRVGDAFALLQGVKEPATPVAFARAVGRAGESLTLTTLGEADPSLAD
                     MATLIVIGSSQTRFIKRDGARAWMVTPRAYGDRR"
     gene            complement(1099..1854)
                     /locus_tag="VKU03_09130"
     CDS             complement(1099..1854)
                     /locus_tag="VKU03_09130"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: protein motif:HMM:NF014613.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HLJ71467.1"
                     /translation="MKPLRLLILGGTSEANALARRIADEPDIAPTLSLAGVTARPKPA
                     AIPQRVGGFGGAEGLAAYITRKRIDAVVDATHPFAARMSANAALACEATETPLVVFTR
                     PPWTREPGDRWVEVGTMDDAVEALGEQPRTVFLTQGRLQLAAFGRAPQHRYVVRAIDR
                     PADSGALPHAKLILTRGPFSLDDELALMKRERVEALVTKNSGGHATYAKIEAARALGI
                     EVVIMRRPPAPEAETLHDLGAVMGWIAAHRLSP"
     gene            1866..>2427
                     /gene="cbiE"
                     /locus_tag="VKU03_09135"
     CDS             1866..>2427
                     /gene="cbiE"
                     /locus_tag="VKU03_09135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HLJ71468.1"
                     /translation="MSGERWLGLVGIGEDGIDSLTPAARRLLAQAALVVGGKRHLALA
                     GPLKAETLAWPSPIENALDVIEAHRGRSVCVLASGDPFFFGVGAVLTRRFEADEVIAI
                     PAPSAFALAASRLGWSEQDCALLSLHGRPLEAIVPHLHDGARILALSWDGATPAKLAA
                     LLAARGMGRSKLTVCEAMGGGNERIRT"
BASE COUNT          339 a          849 c          858 g          381 t
ORIGIN      
        1 tacgcgacag tttcgaggtc actatcgtgc ctggcgtgtc cagcatctgc ggcgcctggg
       61 gagcggccgg cgagccgatg acctggggcg acgatgcgct tgtcgtcctc cccgcgacgc
      121 ttacgctcga cgagctcact cggcgcctca ggctggccga cgcggcggtg atcatgaaga
      181 tcggccgcaa tttcgcgcgc gccaagcaag ctctcgtcgc cgcgggcatg gcggagcggg
      241 caatctatgt cgagcgcgcg accatggcgg gcgaggccgt gacgcctctc tgtcagaagc
      301 gggacgacgc cgcgccctat ttctcgttga tccttgcgcc cggcaggggg cggcggccgt
      361 gaccgggcgc gttgcaatcg tcgggctagg tccgggctca gccgagtgga tgacgccgga
      421 agcgaccgcg gcggtcgcgg cggcgagcga catcgtcggc tatggcccct acctcgatcg
      481 cctgcgcttg cgaccggatc agcgcgctca cccaagcgac aaccggcatg aactggagcg
      541 cgcccggttc gccttgacgc ttgcggcgga aggccgcgcg gttgcggtcg tctccggagg
      601 cgatcccggc gtattcgcga tggcggcggc ggtcttcgag gcgatcgagg aagcgcccga
      661 gtggcgccgc ttcgacattc gggtcgcgcc cggagtgacc gcgatgcagg cggccgccgc
      721 ccggctcggc gcgccactcg gccacgactt ctgcgccatc tcgctctccg acaatctcaa
      781 gccgtgggcg atcgtcgagc gccgcctccg cgcggcggcg gagggcgact ttgtcatcgc
      841 tctttacaat cccgcgtcgc gggcgagacc gaggcgcgtt ggcgacgcct tcgccttgct
      901 gcaaggcgtg aaagagcctg ccacgccggt cgccttcgcc cgcgcggtcg ggcgcgctgg
      961 cgagtcgctg accctgacga cgctcggcga agccgacccg agcctcgccg acatggcgac
     1021 gctcatcgtt atcggctcga gccagacgcg cttcatcaag cgcgacggcg cgcgggcctg
     1081 gatggtgacg ccgcgcgcct atggggacag gcgatgagcg gcgatccagc ccatgaccgc
     1141 gccgagatcg tgcagcgttt cggcttccgg cgcgggcggc cggcgcatga tgaccacctc
     1201 gatcccaagc gcgcgcgccg cctcgatctt ggcgtaggtc gcatggcccc cgctgttctt
     1261 ggtcaccagc gcctcgaccc gctcgcgctt catcaacgcc agttcatcgt cgagcgagaa
     1321 cggcccgcgc gtaagaatca gcttggcatg gggcagagcg ccggaatccg ccggccggtc
     1381 gatcgcccgt acgacgtagc gatgctgcgg cgctcgtccg aaggcggcga gctgaagccg
     1441 cccttgcgtg aggaaaaccg ttcttggctg ctccccaagc gcctcgaccg cgtcgtccat
     1501 cgtcccgacc tcgacccatc gatcgccggg ctcgcgcgtc cagggcgggc gggtgaagac
     1561 gacgagcggt gtttccgtcg cctcgcaggc caaggcggcg ttggccgaca tgcgggcggc
     1621 gaagggatgc gtcgcgtcga ccaccgcgtc gatgcgcttg cgcgtaatgt aggccgccaa
     1681 gccctcggcg ccgccgaagc cgccgacgcg ctgcgggatc gccgccggct taggcctggc
     1741 ggttacgccc gcaagggaca gggtaggcgc aatatccggc tcatcggcga tccgccgcgc
     1801 gagggcgttt gcctccgacg ttccgccgag aatgagcaga cgaagaggct tcagagggcg
     1861 gctccgtgag cggcgaacgc tggcttggcc tcgtcggcat cggcgaggac gggatcgaca
     1921 gcctcactcc tgccgcgcgg cggctcctcg cgcaagcggc cttggtggtc ggaggcaagc
     1981 gtcatcttgc gctcgccgga cctctgaaag ccgagaccct cgcctggccc tctccgatcg
     2041 agaacgcgct cgacgtcatc gaagcccatc gcggccgatc cgtgtgcgtg ctggcgagcg
     2101 gcgatccgtt cttcttcggc gtcggcgccg tcctgacgcg ccgcttcgag gccgacgaag
     2161 tgatcgcgat tccggcgccc tccgccttcg cgcttgccgc ttcgcggctc ggctggagcg
     2221 agcaggattg cgcattgctc tcgctgcacg gccggccgct cgaggcgatc gttccgcatc
     2281 tccacgacgg cgcgcgcatt ctcgcgctct catgggacgg cgccactccg gcgaagctcg
     2341 cggcgctcct cgccgcgcgc ggcatgggac gatcgaagct caccgtgtgc gaggcgatgg
     2401 ggggcggaaa cgagcgcatt cggacgg
//
LOCUS       DATGJW010000300         2264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1195845, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000300 DATGJW010000000
VERSION     DATGJW010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2264
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1195845"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..323)
                     /locus_tag="VKU03_09140"
     CDS             complement(<1..323)
                     /locus_tag="VKU03_09140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CTP synthetase"
                     /protein_id="HLJ71469.1"
                     /translation="MARYIFITGGVVSSLGKGLASAALGTLLQARGFKVRLRKLDPYL
                     NVDPGTMSPYQHGEVFVTDDGAETDLDLGHYERFTGRPATRQDNITTGRIYQEILTKE
                     RRGDYL"
     gene            complement(450..1754)
                     /locus_tag="VKU03_09145"
     CDS             complement(450..1754)
                     /locus_tag="VKU03_09145"
                     /EC_number="3.6.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177954.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HLJ71470.1"
                     /translation="MDNFLALGLAAPLVSALDKIGYAKPTPIQARAIPALLAGRDLIG
                     IAQTGTGKTAAFALPILDRLLANPRRAPQGGARVLILSPTRELASQIAQSFRDLSQGL
                     NLSIAVVFGGVPHGAQIRALARGLDVLVATPGRLVDHLDAMVANLKGVEYFVLDEVDR
                     MLDLGFIKPIRRVVRDLPAKRQNLFFSATMPSDIRRLAGELLRNPVEVAVTPVAKTAD
                     RVEQRVILVDEPRKRDMLVELFKDEAMARAIVFTRTKRGADRVARALEEAGVDALAIH
                     GNKSQGQRERALAAFRGGAARALVATDIAARGIDIDGVTHVVNFELPEVAEAYVHRIG
                     RTARAGREGVAISLCAPADRDLLRGIEKLTRLAIPSVDRRGPKSPAPAPVVRKAAPPS
                     PNRPGRAPQRDARSHKQGSLMRGSRPGRRPWAQSPARAVQAV"
BASE COUNT          410 a          702 c          768 g          384 t
ORIGIN      
        1 agataatccc cgcgacgctc tttggtcagg atttcctggt agatgcggcc ggtggtgatg
       61 ttgtcttggc gtgttgccgg acggccggta aagcgctcgt agtggccgag gtcgagatcg
      121 gtttccgcac catcgtcagt gacgaagacc tcgccgtgct gatacggcga catcgtgccc
      181 gggtcgacgt tgaggtaagg atcgagcttg cgcaggcgca ctttgaagcc gcgcgcctgc
      241 aaaagcgtgc cgagtgccgc tgacgcaaga cccttgccga gtgaggaaac cacgccgccg
      301 gtgataaaaa tataccgcgc catgggagcc gacctctagc cgatccgatt cgattcggcg
      361 agggttgatt tcggagcgcc cgccggactc gtttaaccta ttgatttcta ttctcttcac
      421 gatgtggaaa agcggcggcc cgggacgctc tagacggcct ggacggctct cgccggggac
      481 tgcgcccacg gacggcggcc cggccgcgag ccgcgcatca gcgagccttg cttatggctt
      541 cttgcgtcgc gctgcggggc gcggccgggc cgattgggcg acggcggcgc ggccttgcgc
      601 acgaccggcg caggcgcagg acttttgggc ccgcggcgat cgacgctcgg aatcgcgagc
      661 cgcgtcaatt tctcgatccc gcgcaaaaga tcgcgatccg ccggcgcgca gagcgagatc
      721 gcgacgccct cgcggccggc gcgcgcggtg cggccgatgc gatgcacata ggcctccgcc
      781 acctccggca attcgaaatt gacgacatgg gtgacgccgt caatgtcgat gccgcgcgcc
      841 gcgatgtcgg tcgcgaccag cgcccgcgcc gcgccgccgc gaaaggcggc gagcgcgcgc
      901 tcgcgctggc cctggctctt attgccgtga atggcgagcg cgtcgacgcc ggcttcctcg
      961 agcgcgcgcg ccactcggtc ggcgccgcgc ttggttcggg tgaagacgat cgcgcgcgcc
     1021 atcgcctcat ccttgaagag ttcgaccagc atgtcgcgct tgcgcggctc atcgacaaga
     1081 atgacgcgct gctcgacgcg atccgccgtc ttggcgacag gggtcaccgc gacttctacg
     1141 gggttgcgca gcaattcccc cgccaatcgc ctgatgtcgc tcggcatggt cgccgagaag
     1201 aacaggttct ggcgcttggc cggcaaatcg cgaacgacgc ggcgaatcgg cttgataaag
     1261 ccgagatcga gcatccgatc gacctcgtcg agcacgaaat attcaacgcc cttcagattg
     1321 gcgaccattg cgtcaagatg atcgacgagc cgtcccggcg ttgcgacaag cacgtcgagc
     1381 ccgcgggcca gcgctctgat ctgcgcgcca tgcggcacgc cgccgaacac gacggcgatc
     1441 gaaagattga ggccttgcga aagatcgcga aagctctgcg cgatttggct cgccaattcg
     1501 cgcgtcgggc tgaggatcag gacgcgggcg ccgccttgcg gcgcccggcg cggattggcg
     1561 aggaggcgat cgagaatcgg cagcgcgaag gcggccgtct tgccggtgcc ggtctgagcg
     1621 atgccgatca agtcgcggcc ggcgagaagc gcgggaatgg cgcgcgcttg aatgggcgtc
     1681 ggctttgcgt agccgatctt atcgagcgcg gaaacgagcg gcgcggcgag gccgagcgca
     1741 agaaaattat ccaagaaacg agacctttaa gtgatccgca aaggcgcgcg ccatcggcga
     1801 cgcaagcgcg gacgtttttt cgggagacgc cctgcgtggt ccgggcaatc gaacggaaga
     1861 tcagcggcgt tggtcggaga gctttagccc gtcacgcaac cttcacgccc agaaccgcgt
     1921 gaggctcagc cggcagcggt tgccgatgat atgcgggtga agccgggaaa agtcaatgga
     1981 tgggtctggg cctagagggc cattaggaat agctttcgcc ctatctagag atgtcgggca
     2041 aaagacgcat gaagggaaga tcagcgcttg cattcgccct agccgccctg ccgctcggag
     2101 gttgccagac ggtcagcttg gtcatcggcg accatgcgcc ggccgtaccc ttgtccaagg
     2161 ccgagataaa gcgctgcctg gagctgccgc ataaagtcgg cgaccgcatg gtgtcgcccg
     2221 taccgaggcc aggccatgtg ttcaagccgg gctacaactt ttcg
//
LOCUS       DATGJW010000301         5790 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_680107, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000301 DATGJW010000000
VERSION     DATGJW010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5790)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5790)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5790
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_680107"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..112
                     /locus_tag="VKU03_09150"
     CDS             <1..112
                     /locus_tag="VKU03_09150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007609454.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase family protein"
                     /protein_id="HLJ71471.1"
                     /translation="VIGFKRGTGGTSGVAYLKRMLEVELFPELWRVRTAL"
     gene            complement(145..318)
                     /locus_tag="VKU03_09155"
     CDS             complement(145..318)
                     /locus_tag="VKU03_09155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71472.1"
                     /translation="MAQILHVSGSAIPRLYSAGELGSIFVNYPQSGANLSECIAFGRI
                     AGENAAREEPWTS"
     gene            complement(269..1648)
                     /locus_tag="VKU03_09160"
     CDS             complement(269..1648)
                     /locus_tag="VKU03_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013086.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding protein"
                     /protein_id="HLJ71473.1"
                     /translation="MKSASAAAAQSQRRWDFSADIVIIGYGGAGAVAAISAHDCGAAT
                     LVLEKQERSRHSSNTQLSLGAILLPQNEADATALMNLAGRLNVERPETLDIDQEIIAA
                     WARYAASNAEWLMKLGAQGFTPMSEPGRKADWPGLVSMKVNHLADRNGERLYGDRLFA
                     FLDRSVTARAIDVMWGTPASALIRSDRGEVIGVKAENERGPIAVLAKRAVILACGGFE
                     HNDRLLRTYLPIAPLAVGGNPANTGDGLRMAQEIGADLWHMAVMNGSLKMKFPDFPIA
                     FEENFSQGGFIAVDRHGRRLKAENRLIGYSEFWSAAQYDTSSYIWPRIPVHYVFDEKR
                     RRAGPIVFTEFGAAGPLGLYSWSADNSAEIRRGWIESADTLEILARKIGINGPNLRDE
                     IERFNEAARNGSDCPLGRAASSIAPIDTPPFYAVALWPGAEQHVWRSAPKRLGADPAR
                     LRLGNPPAL"
     gene            1759..2553
                     /locus_tag="VKU03_09165"
     CDS             1759..2553
                     /locus_tag="VKU03_09165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008595505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71474.1"
                     /translation="MTRAAAPPINNLLDLRGKTALVTGAAGGIGRGIARRLAEAGAAV
                     GLHHRSGRERAEETAQEIRKAGGRAVAVAAELTDVASAEAAVQTAMKELGPIDILVNN
                     AARQTHALFEEMALDEWRGMLAANLDGVFIVTKFVTAQMIGRGVGGAVVNIASIEGLQ
                     PAPTHGHYAASKAGLIMYTRAAALQFGRHNIRVNAVSPGLIRRDGIETAWPEGVKRWM
                     AAAPLGRMGEDEDVADAVLFLASPASRWITGANLVVDGGVTTTPAF"
     gene            2561..3349
                     /locus_tag="VKU03_09170"
     CDS             2561..3349
                     /locus_tag="VKU03_09170"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ71475.1"
                     /translation="MTIFSDTALRGRSILVTGAGRGIGAGIAEAALAAGAAVALADLN
                     GELAQETARRLDPSGARTFACAADVADPSSLARAAEAAAARLGALSGWVNNAGIVQMG
                     PAKDFSPEAWRREFEINVGGVMNGAQAAYRAFAGQGGAIVNIASNAGKVGFPNMAAYN
                     ATKAAVINLTRSLAREWAAERINVNAVCPGSVATPMLRGVADRLSAETGQSSDTLFAG
                     MIPAQLGRHIQPIEVGRVVVFLLSEAAEIIRGQSINVDGGETPY"
     gene            complement(3353..4882)
                     /locus_tag="VKU03_09175"
     CDS             complement(3353..4882)
                     /locus_tag="VKU03_09175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007608090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HLJ71476.1"
                     /translation="MRTLIRQATILAMGGPHGDEPFTGDILVEDRSIAAIGPNLDLAG
                     GASVIDGRDRLVMPGLINGHLHSSEQFFKGRYEKMPLEVWLLYAYPLLMGPPIPERLL
                     YLRSMLVAIESIRGGVTTICDCFFDPPTLSLDRLGLVFSAYEAAGLRANITNSIINIS
                     VLDTLPFARDIVPAELQALLAGGTMVAGAAYADYCESAFAAFDRRAGRLRYMISPSAP
                     QRCTVDLLEACSEMALRHRVPFHTHVLETKTQAVTGPDLFGKTLIRYLHDLGLLNRNV
                     TIAHSVWVDDEDMALMGAASCSVVHNAISNQKLGAGVAPLRRLLDAGVTIALGTDGAS
                     SNDTLRIFDVMRVAALIHAVSGPDYSRWLGAGDILHAATIGGARSAMLEDETGSLEAG
                     KRADLIVLRTDNIAFTPMNDPRKHLVYCENGSSLELVMIDGEIVLRDGELTRIDESEI
                     LREAREAVPAYLADHRRIEERNRVFEPYFAEIHRRATLRDIGLNRYAGDMPAWPGANR
                     A"
     gene            complement(4879..5316)
                     /locus_tag="VKU03_09180"
     CDS             complement(4879..5316)
                     /locus_tag="VKU03_09180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ71477.1"
                     /translation="MDFAQRHAISDVCILCADIERSIRFYVDKLGFKLAHRAEGFADF
                     SGAGLTLALWEIDHISRHVGISNARGLGAHKACIAVKLPSRDELDASYRELSAKGVAF
                     FAPPADYPWNATCCYFTGPDDEVWELYAWREGGAPGKLESVRL"
     gene            complement(5324..>5790)
                     /locus_tag="VKU03_09185"
     CDS             complement(5324..>5790)
                     /locus_tag="VKU03_09185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014528769.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71478.1"
                     /translation="VFAFWALLRRTRFGLQLAASGEHPFAARSVGVSPARMRLIALCL
                     GGVLCALGGAELALGSLTIFAYGMTAGRGFMAFSAVIFGAGHPIASVAASLFFAIVGA
                     LGIRAQLVFGDRVPNDLLLALPYIATVIGIWISARLRGGAKAASAFGELRDY"
BASE COUNT         1047 a         1839 c         1941 g          963 t
ORIGIN      
        1 cgtcatcggc ttcaagcgcg gcaccggcgg cacgagcggc gtcgcctatc tcaagcgcat
       61 gctcgaagtc gagctgtttc cggaattgtg gcgcgtgagg accgcgctgt agcgcctatt
      121 tgcaatgagc ccatgcgacc gtccctagct ggtccacggc tcctcgcggg cggcgttttc
      181 tccggcgatt cggccgaagg cgatgcattc gctcaaattg gcgccgctct ggggatagtt
      241 gacgaagatc gagccaagct cgccggcgct atagagccgg gggattgccg agccggagac
      301 gtgcaggatc tgcgccaagg cgtttcggcg cggaccgcca aacgtgttgt tcagcccccg
      361 gccagagagc gacggcgtag aaggggggcg tgtcgatcgg cgcgatcgag gaggcggccc
      421 ggccaagcgg gcaatctgag ccgtttcgcg cggcttcatt aaagcgctcg atttcgtccc
      481 tgagattggg gccgttgatt ccgatctttc tcgcgaggat ttccagcgtg tcggcgctct
      541 caatccagcc gcgccgaatt tcggcgctgt tgtctgcgct ccaggaatag aggccgagcg
      601 gtccggccgc gccgaattcg gtgaagacga tcgggcctgc gcgacgccgc ttttcgtcga
      661 agacatagtg cacgggaatt cgcggccaaa tgtacgacga cgtgtcgtat tgggccgcgc
      721 tccagaattc ggaatagccg atcaagcggt tttcggcttt gaggcggcgg ccatggcggt
      781 caacggcaat gaagccgcct tgtgaaaaat tctcttcgaa ggcgatcgga aaatcgggaa
      841 acttcatctt cagagagccg ttcatcacgg ccatgtgcca gagatcggcg ccgatctctt
      901 gcgccatgcg aagcccgtcg ccggtatttg ccgggtttcc gccgacggcc agcggcgcaa
      961 tcggcaaata ggttcttaag agccggtcat tgtgctcaaa gccgccgcag gcgaggataa
     1021 ccgcccgctt ggcgagcacc gcgatcggcc cgcgttcgtt ttcggctttc acgccgatca
     1081 cctcgccgcg atcgctgcgg atcagcgccg aggccggcgt tccccacatt acatcgatcg
     1141 cccgcgccgt gaccgaccga tcgagaaaag cgaacaaacg gtctccgtac aggcgctcgc
     1201 cgttgcgatc cgcgaggtga ttgaccttca tggaaacaag tcccggccag tcggctttgc
     1261 gtccgggctc gctcatcggc gtaaagccct gggcgccgag tttcatcaac cattcggcat
     1321 tgctcgcagc gtagcgagcc caggcggcga tgatctcctg atcaatatcc agcgtctcgg
     1381 gtctttcgac gtttaggcgg ccggctaggt tcatcaaggc cgtcgcgtcc gcttcgttct
     1441 gcggaagcaa gatcgcgcca agcgacaatt gcgtattcga actgtggcgt gatcgctcct
     1501 gcttctccag gacgagagtc gccgcgccgc aatcgtgagc gctgatcgcc gcaaccgcgc
     1561 ccgccccgcc atatccgatg atgacgatat cggcgctgaa gtcccaacgc cgttgggact
     1621 gcgcggccgc agcggacgcc gatttcatgg agcgtttctc ccagctggct gcgtgtcgcg
     1681 cccttgaaac caatgcgaga ttggggccta attcgcaccg ctcgtttgta gcgccaagcc
     1741 gattccagca ggtgagccat gacccgcgcc gctgcgccgc caatcaataa cctcctcgat
     1801 ctgaggggca agacggcttt ggtcaccggc gccgccggcg gaatcgggcg aggaatcgcc
     1861 cggcgtttgg cggaggcggg ggccgcggtc gggctccacc atcggagcgg tcgcgagcgc
     1921 gccgaggaga cggcgcaaga gatccgcaaa gcaggcggca gggcggtcgc cgtcgcggcc
     1981 gagttgaccg acgtcgcatc cgcggaggcc gcggttcaga cggcgatgaa agagctcggt
     2041 ccgatcgaca tcctggtcaa caatgcggcg cgccagactc acgccttgtt cgaagagatg
     2101 gcgctcgacg agtggcgcgg catgctcgcg gccaatctcg acggggtctt catcgtcaca
     2161 aaattcgtga cggcgcagat gatcggccgc ggcgtcggcg gcgccgtcgt caacattgct
     2221 tcgatcgaag gtttgcagcc ggcgccgacc cacggccatt acgctgcctc gaaggccgga
     2281 ttgatcatgt atacgcgcgc cgccgcgctc caattcggcc gccacaatat ccgcgtcaac
     2341 gccgtctcgc cgggcctgat ccgccgcgac ggcatcgaga ccgcctggcc ggaaggcgtg
     2401 aagcggtgga tggcggccgc gccgctcggc cgaatggggg aggacgagga cgtggccgac
     2461 gcggtgctct tccttgcctc gccggcttcg cgctggatca ccggcgccaa tctcgtcgtc
     2521 gatggcggcg taacgaccac tccagctttc tgaggcgcgg atgacaatct tctcggatac
     2581 ggcgcttcgc ggacgaagca tcctggtcac cggcgcgggg cggggcatcg gcgccggaat
     2641 cgccgaggcg gcgctcgcgg ccggcgccgc cgtcgctctg gccgacctga acggcgaatt
     2701 ggcgcaggag acggcgcgcc ggctcgaccc gtccggcgcg cgcacattcg cctgcgcggc
     2761 ggatgtcgca gacccttctt cgctcgcccg ggcggccgaa gccgcggcgg cgcgcctcgg
     2821 cgcgctgagc ggctgggtga acaatgccgg catcgttcag atggggccgg cgaaagactt
     2881 ctcgccggag gcttggcgtc gcgagttcga gatcaatgtc ggcggcgtga tgaacggcgc
     2941 acaggcagcg taccgcgctt tcgctggcca gggcggggcg atcgtcaaca tcgcctccaa
     3001 cgccggcaag gtcggctttc cgaacatggc ggcgtataac gcgaccaagg cggcggtgat
     3061 caacctgacg cgttctctgg cgcgcgaatg ggccgccgag cggatcaatg tgaacgcggt
     3121 ttgtcccggc agcgtcgcca cgccgatgct gcgcggcgtc gccgaccgcc tctccgccga
     3181 gaccggccag agcagcgaca cgctgttcgc cgggatgatc ccagcgcagc ttgggcggca
     3241 tatccagccg atcgaagtcg gccgcgtcgt cgtcttcctg ctgagcgagg cggccgagat
     3301 cattcgcggc cagtcgatca atgtcgatgg cggcgagacg ccctattgag gttcaggcgc
     3361 gattggcgcc cggccaggcc ggcatgtcgc ccgcatagcg gttgagcccg atatcgcgca
     3421 acgtcgcgcg ccggtgaatt tcggcgaaat agggctcgaa gacgcgattc ctctcctcga
     3481 tgcgcctgtg atcggcgaga taggcgggga ccgcctcgcg cgcttcgcgc aggatttcgc
     3541 tttcgtcgat gcgcgtcagc tcgccgtcgc gcagcacgat ctcgccgtcg atcatgacga
     3601 gttcgagcga ggagccgttc tcgcaataga cgagatgttt gcgcggatcg ttcatcgggg
     3661 tgaaggcgat gttgtcggtc ctgagcacga tgagatcggc ccgtttgccg gcttccagcg
     3721 atccggtctc gtcctccagc atggcgctgc gcgcgccgcc gatcgtcgcg gcgtgcaaaa
     3781 tgtcgccggc gcccaaccag cgcgaataat ccgggcctga gacggcgtga atcagcgcgg
     3841 cgacgcgcat gacgtcgaaa atgcgcaacg tgtcgttgct cgatgcgcca tcggtgccga
     3901 gcgcgatcgt gacgccagcg tcgagcagcc gcctcagcgg cgcaacgccc gcgccgagct
     3961 tctgattcga aatcgcgttg tggacgacgg agcagctcgc cgcgcccatc agcgccatgt
     4021 cctcgtcatc gacccagact gaatgggcga tcgtcacatt gcggttgagg aggccgagat
     4081 cgtgcaagta gcgaatgaga gtcttgccga acagatccgg ccccgtaacg gcctgggtct
     4141 tcgtctccag tacatgggta tggaagggaa cgcgatggcg caacgccatc tcgctgcagg
     4201 cttcgaggag atcgaccgtg cagcgctgcg gcgccgaggg cgaaatcatg tagcgcaacc
     4261 ggccggctct gcggtcgaag gcggcgaaag ccgattcgca ataatcggca taggcggcgc
     4321 cggcgaccat cgttccgccc gcaagcagcg cctgcagctc cgccggcacg atgtcgcggg
     4381 cgaatggcag cgtatcgaga accgagatgt tgatgatcga gttggtgata ttggcgcgca
     4441 ggccagccgc ttcataggct gagaagacca ggccgagccg atcgagcgac agggtcggcg
     4501 gatcgaagaa gcaatcgcag attgtggtga cgccgcccct gatcgattcg atcgcgacca
     4561 acatcgagcg cagatagagg agccgctccg ggatcggcgg acccatcagc agcggatagg
     4621 cgtagagcag ccagacttcg agcggcatct tttcgtaccg ccctttgaag aactgctcgc
     4681 tggaatgaag atggccgttg atgagccccg gcatgacgag ccgatcacgt ccgtcaatca
     4741 cgctggcgcc gccggccaaa tcgagattcg ggccgatcgc ggcgatgctt ctatcctcga
     4801 caaggatatc gcccgtgaag ggctcatcgc catgcgggcc gcccatggcc aggatcgtcg
     4861 cctggcggat gagagtcctc atagtcgtac tgactccaat ttccccggcg cgccgccctc
     4921 gcgccaagca tagagctccc agacttcgtc gtcggggccg gtgaagtagc agcaagtggc
     4981 gttccaagga taatcggccg gcggcgcgaa gaaggcgacc cccttggccg agagctcgcg
     5041 atagctggcg tcgagctcgt cgcgggaagg caacttgacc gcgatgcaag ccttgtgcgc
     5101 gccgagcccg cgcgcgttgg aaatgccgac atgccggctg atatgatcga tctcccatag
     5161 agccagggtc agcccggcgc cggagaaatc ggcgaagccc tcggcgcgat gcgcgagctt
     5221 gaagccaagc ttgtccacat agaaccggat cgagcgctcg atatcggcgc aaaggatgca
     5281 gacgtcgctg atcgcgtggc gctgggcgaa atccatctct tcctcaatag tcgcgcaatt
     5341 cgccgaacgc gctcgccgcc ttggcgccgc cgcgcaaccg cgcgctgatc catatgccga
     5401 tgacggtggc gatatagggc aacgccagga gaagatcgtt cggcacccgg tcgccgaaca
     5461 cgagctgcgc gcggatgccg agcgccccaa cgatggcgaa aaagagcgag gcggcgaccg
     5521 aggcgatcgg atggccggcg ccgaaaatga ccgccgaaaa cgccatgaag ccgcgcccgg
     5581 cggtcatgcc ataggcgaag atcgtcaggc tgccgagcgc gagctcggcg ccgccaaggg
     5641 cgcaaagcac gccgccgaga cagagcgcga tcagccgcat ccgcgccggg ctgacgccga
     5701 cgctgcgcgc cgcgaagggg tgctcgccgg aggcggcaag ctgaagcccg aaccgggtgc
     5761 ggcggagaag cgcccagaag gcgaaaacga
//
LOCUS       DATGJW010000302         3455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1302943, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000302 DATGJW010000000
VERSION     DATGJW010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3455
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1302943"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(438..863)
                     /gene="cueR"
                     /locus_tag="VKU03_09190"
     CDS             complement(438..863)
                     /gene="cueR"
                     /locus_tag="VKU03_09190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006878 - intracellular copper ion
                     homeostasis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Cu(I)-responsive transcriptional regulator"
                     /protein_id="HLJ71479.1"
                     /translation="MNIGQAATASGVSAKMIRHYEAIGLLRPAARKENSYRDYGGHDV
                     HELRFIGRARKLGFSIDEIAALLALWRDKNRPSREVKQVAAAHLADLETRIAEMQSMA
                     ASLRDLVRTCRGDDRAECPILNDLEGERGDAARRRGLAS"
     gene            951..3362
                     /locus_tag="VKU03_09195"
     CDS             951..3362
                     /locus_tag="VKU03_09195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011992950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HLJ71480.1"
                     /translation="MTAHIHHPHHGRHEPADRNAPMPEGKARDPVCGMSVTIATAKHK
                     AEYAGRSYYFCSAGCREKFVAEPKRFLANPEAEASAPAKHQHAHSHGRPYAQPRKAPA
                     GAIYTCPMHPEIRQAGPGSCPICGMALEPEIATADEGPSAELVDMTRRFGIALALALP
                     VVAVEMGGHVVDLHGMLAQTSNWIQLVLTTPVVLWAGWPFFERGWASLKTRRLNMFTL
                     IAMGVGVAWLYSVVATVAPGWFPPAFRGAHGAVAIYFEAAAAITVLVLLGQVLELRAR
                     EQTGGAIRALLDLAPKTARRVRADGGDEEIALDWVAVGDLLRVRPGEKVPVDGDIVEG
                     SGAVDESMITGESLPIAKKPGDKAIGGSLNQSGAFILRADKVGADTMLAQIVRMVAEA
                     QRSRAPIQRLADQVSGWFVPLVILVAIVAFAAWAAFGPEPRFAYGLVAAVSVLIIACP
                     CALGLATPMSIMVGVGRGARAGVLIKNAEALERFETVDTLVVDKTGTLTEGKPKVVAL
                     RAFGVGENELLRLAASLEQNSEHPLAAAIVAAAGERKIALAKTQDFESAAGKGAAAIV
                     DGRRVVVGNRAMMQGAGIDASGLESAADEWRGEGATAIFVGLDGDAAGVIAIADPIKA
                     TTAAAIAALREAGIRVVMLTGDNRQTAEAVARKLGIADIEADILPQEKSRVVERLRKE
                     GRVVAMAGDGVNDAPALAAADIGVAMATGTDVAIESAGVTLLRGDLQGLVAARRLSQA
                     TMRNIRQNLFFAFVYNAAGVPIAAGVLYPVFGLLLSPIIAAAAMSLSSVSVVGNALRL
                     RAARL"
BASE COUNT          553 a         1138 c         1212 g          552 t
ORIGIN      
        1 cttcagcttc ttccgctacg ccgccatcgc ccagggcgtc tataagcgcg cgctcgacgg
       61 caacgcgtca aacccgctgc gcggccgcaa gctcggcgag gccgtgccgc aaatggcgcg
      121 gctggcgacg gagatcatcg agaagggcgg gtaaaccccg atcctcctcc gtcgaagacg
      181 gctacggcat gcacacatct tgaagcgcgg cgcgcctgag ccgtgctcgt cgtcaaggcc
      241 aagcgcgctt cgcgcgtcgc tcgccaaagc aggctcagga gaaaatcagt tggcgcggcg
      301 cgatggctcg cgagccttga cggctccgcg cggctgatgt ggccccggcg cgccgagatg
      361 cgtgcatgcc gtagcgaagg gagggggagg cgcggtcagc gaccgcgcct ccagcctcgc
      421 ccgggcgctc gaaccgatta cgatgcgagc ccgcgccgcc gagcggcgtc gccgcgttcg
      481 ccctccagat cgttcaagat cgggcattcg gcgcggtcgt cgccgcggca ggtgcgcacc
      541 aggtcgcgca agctcgccgc catgctctgc atttccgcga tgcgcgtttc gagatcggcg
      601 agatgggccg ccgcgacctg cttcacttcg cggctcggcc ggttcttgtc gcgccagagc
      661 gccagcagcg cggcgatctc gtcgatcgag aagccgagct tgcgggcccg gccgatgaag
      721 cgcaactcgt gcacgtcatg gccgccatag tcgcgatagc tgttctcctt gcgcgccgca
      781 ggccgcagca ggccgatcgc ctcgtaatgg cggatcatct tggcgctgac gccggaggcc
      841 gtcgcggctt gaccgatgtt catggccgtc tcgttttgct tgaccttccc atgatgggaa
      901 ggtgtagctg tggcgcgtca aattcaagag cgaagaagag gcgcagaggg atgaccgcgc
      961 atatccatca cccgcatcat ggccgccacg aaccggccga tcgcaacgcg cccatgccgg
     1021 agggaaaagc gcgcgatccg gtctgcggca tgagcgtgac catcgccacg gccaagcaca
     1081 aggccgagta cgcggggcga tcttattatt tctgctcggc cggttgccgc gagaaattcg
     1141 tcgccgagcc gaagcgcttc cttgccaatc ccgaagcgga ggcgtccgct ccggcaaagc
     1201 atcagcacgc ccattcacac gggcgcccat acgcgcagcc gaggaaagcg ccagccggcg
     1261 cgatctacac ctgcccgatg catccggaga ttcgccaggc cggtccgggc tcttgcccga
     1321 tctgcggcat ggcgctggag ccggaaatcg cgacggcgga tgaggggccg agcgccgagt
     1381 tggtcgacat gacccggcgc ttcggcatcg cgctggcttt ggcgctgccg gtcgtcgcgg
     1441 tcgaaatggg cggccatgtc gtcgacttgc acgggatgct cgctcagaca tccaattgga
     1501 ttcagctcgt cttgacgacg ccggtcgtgc tctgggcggg ctggccgttc ttcgaacgcg
     1561 gctgggcgtc gctgaagacg cgccgtctca acatgttcac gctgatcgcg atgggcgtcg
     1621 gcgtcgcctg gctctacagc gtcgtcgcca ccgtcgcgcc cggctggttc ccgccggctt
     1681 ttcgcggcgc gcatggcgcg gtcgcgatct atttcgaagc ggcggcggcg atcaccgtgc
     1741 tggtgctgct cggccaggtc ttggaattgc gcgcgcgaga gcagaccggc ggcgccattc
     1801 gcgccttgct cgatctcgcg cccaagacgg cgcgccgggt ccgcgccgac ggcggcgacg
     1861 aagagatcgc gctggattgg gtggcggtcg gcgatctcct gcgggtgcgg ccgggcgaga
     1921 aagtcccggt cgacggcgac atcgtcgagg ggagcggcgc ggtcgacgaa tccatgatca
     1981 ccggcgaatc gctgccgatc gccaagaagc cgggcgacaa ggcgatcggc ggctcgctca
     2041 atcagagcgg cgccttcatc ctgcgcgccg acaaggtcgg cgccgacacg atgctggcgc
     2101 agatcgtcag gatggtcgcc gaggcgcagc gcagccgcgc gccgatccag cgcctcgcgg
     2161 accaggtctc cggatggttc gtacccttgg tgatcctggt cgcgatcgtc gccttcgccg
     2221 cctgggcggc tttcggccca gagccgcgct tcgcctacgg cctggttgcg gcggtgtcgg
     2281 tgctgatcat cgcctgtcct tgcgcgctcg gcctggcgac gccgatgtcg atcatggtcg
     2341 gcgtcgggcg cggcgcgcgc gccggcgtgc tgatcaagaa cgccgaggcg ctggagcgct
     2401 tcgagacggt cgatacgctc gtcgtcgaca agaccggcac gctgaccgag ggcaagccga
     2461 aagtcgtcgc actccgcgcc ttcggcgtcg gtgaaaacga gttgctgcgc ctagcggcga
     2521 gtctcgaaca aaacagcgaa catcccttgg cggcggcgat cgtcgcggcg gcgggcgagc
     2581 ggaaaatcgc gctcgccaaa acgcaggatt tcgagtcggc cgccggcaaa ggcgcggccg
     2641 cgatcgttga cggacgacgc gtcgtggtcg gcaaccgcgc tatgatgcaa ggcgccggga
     2701 tcgacgcgtc agggctcgaa agcgcggctg acgaatggcg cggcgagggc gccaccgcga
     2761 ttttcgttgg gctcgacggc gacgcggccg gcgtgatcgc gatcgccgat ccgatcaagg
     2821 cgacgaccgc cgccgcgatc gcggctttgc gcgaggccgg aatacgcgtc gtcatgctga
     2881 ccggcgacaa tcgccaaacg gctgaagccg tggcgcgcaa gctcggcatt gccgatatcg
     2941 aggccgatat cttgccgcag gaaaagagcc gcgtcgtcga gcggctgcgc aaagagggcc
     3001 gcgtcgtcgc gatggccggc gacggcgtca acgacgcgcc ggcgctcgcg gccgccgata
     3061 tcggcgtggc gatggcgacg gggacggatg tggcgatcga aagcgccggc gtcacgctgc
     3121 tgcgcggcga tctgcaaggc ctggtcgcgg cgcgccgcct gtcgcaggcg acgatgcgca
     3181 acatccgcca gaacctgttc ttcgccttcg tctacaacgc cgccggcgtg ccgatcgcgg
     3241 cgggcgtgct ctatcctgtc ttcggcctgc tcctgtcgcc gattatcgcc gctgcggcga
     3301 tgtcgctgtc gtcggtgagc gtcgtcggca atgcgctgcg gctgcgggcg gcgcggcttt
     3361 gagcgaatga cgctaggttt acttccagaa cggaaaacgc aaatgggccg tcttcccaac
     3421 gcaaaacaaa tccaagagtg ggaacggaga gaagg
//
LOCUS       DATGJW010000303         4603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_989889, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000303 DATGJW010000000
VERSION     DATGJW010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4603
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_989889"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(207..908)
                     /locus_tag="VKU03_09200"
     CDS             complement(207..908)
                     /locus_tag="VKU03_09200"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLJ71481.1"
                     /translation="MPAWVTPDHLTVAGLIGAVMTAIGYAATNRHPAFYLLACLGLVV
                     NWFGDSLDGSLARYRKIERPRYGYFVDHSVDVINNFLIIGGLGLTPDVGSAAALFALC
                     SYLALSIYVFLLNQVSGELQLTFVNCGPTEIRLGIIAFNLALFLIGPTSLSVLGVTIP
                     LPTAGIGILGAVLAIVFVVSLYKTARNLARESDIEAQAKAAESGRRPGVETISAAPKA
                     FAAPPSPKKGATFAT"
     gene            1199..1951
                     /locus_tag="VKU03_09205"
     CDS             1199..1951
                     /locus_tag="VKU03_09205"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71482.1"
                     /translation="MPKSVLITGASSGIGRALALEYAASGVTLGLIGRDKNRLEDAAE
                     LARAKGAAVSTGTLDVRDADAMSAFLLAFDDEAPVDCVIASAGVTSVTRKEAEAADLL
                     RAKEIFDVNVGGVMNTLAPLAPRMRRRRAGQIGILSSLAAFAPVPNAAAYSASKAALL
                     SFSLALRALYRADGVSVSAICPGFVDTPMTSLYAGRKPGLITAEEAAHRIRRGLERRK
                     AIIAFPLSLYFGARAQEWLPLALRDRIMLAFR"
     gene            complement(2008..3354)
                     /locus_tag="VKU03_09210"
     CDS             complement(2008..3354)
                     /locus_tag="VKU03_09210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330307.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLJ71483.1"
                     /translation="MRRLSEEDRQLIGRVVQARGPEAKAAGGKTADRSALLDFKNLPG
                     FAEQTMLRAAASAMGLRDPYFLVHEGDAGAETVIDGRRLANFSSYDYLGLNHHPAVRE
                     AAKAAIDRYGVSPSASRLVAGERPIHRALETALAEHYSQEACVTYVSGHAANVATIGA
                     LLGPRDLILHDALAHNSIVMGALLSQAQRRAFAHNDMAGLDATLGEIRGQYERVLIAA
                     EGLYSMDGDVCDLPTLVEIKQRHQAWLMIDDAHGLGALGERGFGVFEHFRMAPRGVDI
                     WMGTLSKTLAACGGYIAGPSMLIDYLKHAAGGFVYSVAMPPAIAAAALKALELLHGEP
                     ERVQRLRANGTAFVEAARRAGLDTGASVGASIVPIMTGSSIRAAALSQRLFDRAVNVQ
                     PIIYPAIPERLARLRFFLSSEHRLEQIETVVPIIASEIEAANAMSLKAMARLGSNA"
     gene            complement(3488..3976)
                     /locus_tag="VKU03_09215"
     CDS             complement(3488..3976)
                     /locus_tag="VKU03_09215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLJ71484.1"
                     /translation="MATLKVTASAPPDQPTITMEREFAAPPDAVFRAFSDAEALRIWY
                     GPNGFSITVIAMDFRVGGLFRFTMHAPDGTDFPNRIRYREIAPFERLSYQQGGDSDDD
                     PNAFEVVNVFTRIGDNRTRLVRTMTFPSIDARNAVMKFGAVELGMQTIEKLAAYLEAL
                     GA"
     gene            complement(3963..4316)
                     /locus_tag="VKU03_09220"
     CDS             complement(3963..4316)
                     /locus_tag="VKU03_09220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLJ71485.1"
                     /translation="MTSDPLSATFKALADPTRRAILARLSAGESSVLDLAAPFAMSLP
                     AVSQHLKVLEHAGLISRGREAQWRPCRLEASALKEATDWLDTYRRFWEGSFDKLDRYL
                     KDIQSMQKEKKDGDA"
BASE COUNT          853 a         1463 c         1485 g          802 t
ORIGIN      
        1 gatgaggtgc tcaggcagtt ggagaacgga tcggccgtcg agaccctcgc gccggtccag
       61 ccctattgcc cgttcccgcc gcgagaactg ctgcgccgag ccaagcggcg cggcgtcaag
      121 ctcgccgatt cccgcgatct gcgccgcgcc gtcttcggat agatcaggcg aaacgaatgc
      181 agggcggagc gggctgcctg cgaaaactag gtcgcgaaag tcgcgccctt cttcggagaa
      241 ggcggagccg cgaacgcttt cggcgcagcc gagattgtct caacgcccgg ccggcggccg
      301 ctctcggcgg ctttcgcttg cgcctcaatg tcgctctctc gagcaagatt gcgcgccgtc
      361 ttatagaggc tcacgacaaa gacgatggcc aggaccgcgc ccaatatccc gatcccggcc
      421 gtcggcagcg ggatcgtcac gccgaggacc gagagcgagg tggggccaat cagaaacaag
      481 gccagattga acgcgatgat gcccagccgg atttcggtcg gtccgcaatt gacgaaggtg
      541 agttgaagtt cgcccgaaac ctgattgagg aggaaaacgt aaatcgacag ggcgagataa
      601 ctgcaaagcg cgaacagcgc ggcggccgag ccgacatcgg gcgtaagccc caggccgccg
      661 atgatgagaa aattgttgat cacgtcgacc gagtgatcga cgaaatagcc gtaacgaggc
      721 cgctcgatct tgcggtagcg ggcgagagag ccgtcgagcg aatccccgaa ccaattcacg
      781 accaagccta aacaggccaa cagatagaag gccggatgcc ggttggtcgc cgcatagccg
      841 atcgccgtca tcacggcgcc gatcaggccc gccacggtca gatggtccgg cgtgacccag
      901 gccggcatga cccgacagaa tccgttcaaa agctggcgct cgcgccgggc taagaggttg
      961 gtttgaatcc gctttggcgc ctggaatgac tcagcggcgc cgtcgcccac aattccatcg
     1021 agccgctcaa gcggataggt tgcgagctct gacactggcg ccccaaattg agcggctctc
     1081 cggccgcgtt ttttcaagcc ggcaccataa cggcaaaggt cgccgctggc tatgatccgt
     1141 ttgcaacagc cttcgtttat atcggataaa aatttacccg ccgacgacgg atttgttcat
     1201 gccaaagagc gttctaatca ccggagcaag cagcggaatc ggccgagctt tggcgctcga
     1261 atacgccgcc tccggcgtga ccctcgggct catcggccgc gacaaaaaca ggctcgaaga
     1321 cgccgccgaa ttggccagag ccaagggcgc ggccgtctca acggggacgc tcgacgtgcg
     1381 cgacgccgac gcgatgagcg ccttcctgct cgcctttgac gacgaggcgc cggtcgactg
     1441 cgtcatcgcc agcgccggcg tcaccagcgt gacgcgaaag gaggccgagg cggccgatct
     1501 gctccgcgcg aaggagattt tcgacgtcaa tgtcggcggc gtcatgaaca cgctcgcgcc
     1561 gcttgcgccg cggatgcggc ggcggcgcgc cggacagatc ggaatcctca gttcgctcgc
     1621 cgcctttgcg ccggtgccga acgccgccgc ctacagcgcc agcaaagcgg ccttgttgtc
     1681 cttctcgctc gccctgcgcg cgctctatcg cgccgacggc gtcagcgtca gcgcgatctg
     1741 tccaggcttt gtcgacacgc cgatgaccag cctctatgcc ggtcgcaagc ctggtctgat
     1801 cacggcggag gaggcggcgc accgcattcg ccgcgggctt gagcggcgga aagcgatcat
     1861 cgcttttccg ctgagcctct atttcggcgc ccgcgcgcag gaatggctgc cgctcgcgtt
     1921 gcgcgatcgc atcatgctcg cctttcgttg agcgtttggc cggcgccgat tattcccgct
     1981 cggtcgagcg tctatcgccg agcgcgctca cgcgtttgaa ccgagccgcg ccatcgcctt
     2041 cagcgacatg gcgttcgcgg cctcgatctc gctcgcaatg atcggcacga ccgtttcgat
     2101 ctgctccagc cgatgctcgc tgctcaggaa aaaccgcagc cgcgccagcc tctccggtat
     2161 cgctggataa atgatcggct gcacgttcac ggcgcgatcg aagagccttt ggctcaaagc
     2221 cgcggcccgg atcgagcttc ccgtcatgat cgggacgatc gaggcgccga cgctcgcgcc
     2281 ggtatccaag ccggcgcggc gcgccgcttc gacgaaggcg gtcccattgg cgcgcagtct
     2341 ttgcacgcgc tcgggctcgc catggagaag ctcaagcgcc ttgagcgccg cggcggcgat
     2401 cgccggcggc atcgcgacgc tgtagacaaa accgccggct gcatgtttca aatagtcgat
     2461 gagcatagaa gggccggcaa tatagccgcc gcacgccgcc agcgtcttgg agagcgtgcc
     2521 catccaaatg tcgacgccgc gcggcgccat gcggaaatgc tcgaacacgc cgaagccgcg
     2581 ttcgccaagc gcgccgagcc catgcgcgtc gtcgatcatc agccaggcct gatggcgttg
     2641 cttgatctcg accaatgtcg gcaaatcgca aacatcgccg tccatgctgt agaggccttc
     2701 cgcggcgatc agcacccgct cgtactggcc tcggatctcg ccgagcgtcg cgtcgagtcc
     2761 ggccatgtcg ttatgcgcga aggcgcggcg ctgggcctgc gataaaagcg cgcccatgac
     2821 gatgctgtta tgggcgagcg cgtcgtgcaa aatgagatcg cgcggcccga gcagcgcgcc
     2881 tatcgtcgcg acattggcgg cgtggccgct gacataggtc acgcaggctt cttggctgta
     2941 gtgttcggcc aaggcggttt cgagcgcgcg atgaatcggc cgctcgccgg cgaccagccg
     3001 gctcgccgaa ggcgagacgc cgtagcggtc gatcgccgcc ttcgccgcct cgcgcaccgc
     3061 gggatgatga ttgaggccga gatagtcgta agaggagaaa ttcgccaagc gccggccgtc
     3121 gatgacggtc tcggcgccgg cgtcgccttc gtgaacgaga aaataaggat cgcgcaggcc
     3181 catggccgaa gcggcggcgc gcagcatcgt ctgctcggcg aagccgggca ggtttttgaa
     3241 atcgagcaaa gccgagcgat cggcggtttt gccgccggcc gccttcgcct ccgggccgcg
     3301 cgcctgaacg acgcggccga tcaattggcg atcctcctcg gacaaacggc gcacggcgtt
     3361 ttcctgttcg ggtggtcgga tcgcggcgac ccgatcggct atccaggcgc agccgagacg
     3421 acggaaatgt gactttttgt tgtccgctgg ccgaagatcg ggctttcgcg cgaaaatcgt
     3481 ccggcgctca ggcgccgagc gcctccagat aggccgccaa tttctcgatc gtctgcatgc
     3541 ccagttcgac ggcgccgaat ttcatcaccg cgttgcgcgc gtcgatcgac gggaacgtca
     3601 ttgtccgcac caggcgggtt cgattgtcgc cgattcgcgt gaagacgttg acgacctcga
     3661 acgcgttcgg gtcgtcgtcg ctgtcgccgc cctgctgata ggacaggcgt tcgaatggag
     3721 cgatctcgcg gtatcggatg cggttcggaa agtcggttcc gtccggcgca tgcatcgtga
     3781 agcggaacag gccgccgacg cgaaaatcca tcgcgatcac cgtgatcgaa aagccgttgg
     3841 ggccgtacca aatccgcaac gcttcggcgt cggagaaggc gcgaaaaacg gcgtcgggag
     3901 gcgcggcgaa ttcgcgctcc atcgtgatcg tcggttggtc cggcggcgcg ctcgccgtca
     3961 ctttaagcgt cgccatcttt tttctccttc tgcattgatt gaatgtcttt gagatagcgg
     4021 tcgagcttgt cgaagctgcc ttcccagaac cgccggtacg tgtcgagcca atcggtcgcc
     4081 tctttcagcg cggacgcctc cagccgacag ggccgccatt gcgcctcgcg gccgcgactg
     4141 atcaggccgg catgttcgag cactttcaga tgctgcgaca ccgccggcag gctcatcgcg
     4201 aaaggcgcgg cgagatcgag gaccgaggat tccccggccg aaagacgcgc caggatcgcc
     4261 cgccgggtcg gatcggccaa agccttgaag gttgcgctca gcgggtcgct ggtcatggcg
     4321 tttgcgctgt atttaagaga ttgcttaaat acaacagcca gagttgaggg tcaagggcgt
     4381 tttctcaaaa agagagtaga gcggggctga ttcggatctg acgggtcatc ggccttgcgt
     4441 cggtcgacga gttggctcgc ggaccctctt tgcgaatgtc agcggtcaga gcgctcggcg
     4501 ctagggcgcc cgcgccatta ccgcatggat aaaggcgaag ccgacgatcg agacggtgat
     4561 cagcacatag acgagcagcg cgacaagcac ggcgacggtt ccc
//
LOCUS       DATGJW010000304         3780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1407100, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000304 DATGJW010000000
VERSION     DATGJW010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3780
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1407100"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1184)
                     /locus_tag="VKU03_09225"
     CDS             complement(<1..1184)
                     /locus_tag="VKU03_09225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509209.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family secretion protein"
                     /protein_id="HLJ71486.1"
                     /translation="MSQVKPFPRDRKDDATPAQPAPVPTPAPDAAPPLAPAQRPAPQR
                     AEAPKRRRRGFSRFVLLIVVPALALAGGFYWWLSSGRYVSTDNAYIGADKVLITPQVT
                     GPIVHVDVAEGQHVKAGDKLFEIDPAPYRTALALAKARLEAAKNQYANLQLSFKANAD
                     QVKMSEEAVRVRQADFDRKSQLAKSGAGTQNDKDTSNAALIQAQQILAFVMQQQQSAA
                     IQLGGKPDAPLEEFPAYIEAKAQVEDAERNLRNTEVVAPIPGVATQVPQIQLGRVIAA
                     GTPVFSIVNDRDLWIDANPKESDLTYVRPGLPVTISVDTFPGRTWRGKVGAIAPATGA
                     QFAILPPQNASGNWVKVVQRVPLRVEFNPDQDTSDLRAGMSAVVDIDTGRQRTMAGVW
                     AE"
     gene            complement(1191..1688)
                     /locus_tag="VKU03_09230"
     CDS             complement(1191..1688)
                     /locus_tag="VKU03_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ71487.1"
                     /translation="MPHTSLLAPAPQIAVAKKPMSPAREFVFAMHDVARLLRTLADQR
                     ARALNMTRAQWSVLKRLEMSQGVKQAELAELLDLQPITLARLVDKLAGLGLVERRDDP
                     NDRRANRLYLTEGAAPVLERLNELGEALVGQALNGFDAFEIAALGKSMDRIKTNLKRE
                     LNCRG"
     gene            complement(1895..2215)
                     /locus_tag="VKU03_09235"
     CDS             complement(1895..2215)
                     /locus_tag="VKU03_09235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018644559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71488.1"
                     /translation="MDLAAFRASLDRPAPPAGLSVALQALWWDGRGDFDAAHDLAQRD
                     EGGDGDWVHAYLHRKQGDASNAAYWYRRAGRPFCRDALAAEWEAIASALLAAPAGSAG
                     WVGD"
     gene            2452..2931
                     /locus_tag="VKU03_09240"
     CDS             2452..2931
                     /locus_tag="VKU03_09240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HLJ71489.1"
                     /translation="MERFVSHFTNRFDAKGRISIPAPFRAVLARDGFEGLYLHPSLDA
                     PALDCGGNALLSEIDALLATMPAYSEPRDMLATALLGTSEILRPDSEGRVILSEASRT
                     FAHLTDEAVFVGQGHKFQIWAPDRFRAHLEEAKTRVRQLRAELGARAARSAPAERAE"
     gene            2928..>3780
                     /gene="rsmH"
                     /locus_tag="VKU03_09245"
     CDS             2928..>3780
                     /gene="rsmH"
                     /locus_tag="VKU03_09245"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HLJ71490.1"
                     /translation="MMASRGEAPPPAGGPARHIPALLDEAVAALGVHEGGVYCDGTFG
                     AGGYARAILDRGGRVVAIDRDPEAIAAGQALAASAGGRLALVEGRFGELDAIARSQGF
                     SALDGVVLDIGVSSMQLDEAQRGFSFRNDAPLDMRMGRSGRSAADLLAEEDEQALADI
                     LYHFGEERAARRIARAIVHDREAAPFVSTRQLASLIERVAPARPGEAIHPATRSFQAL
                     RIAVNDELGELARGLVAAEAILKPGGRLAVVTFHSLEDRIVKQFFARRSGRGQAAGRR
                     LPGEKPAP"
BASE COUNT          566 a         1242 c         1320 g          652 t
ORIGIN      
        1 atctcggccc agacgccggc catggtgcgc tggcggccgg tgtcgatgtc gacgaccgcg
       61 ctcatgccgg cgcgcaaatc gctcgtatcc tggtcgggat tgaattcgac gcgcagcggc
      121 acgcgctgaa cgaccttgac ccagtttccg ctggcgttct gaggcggcaa aatggcgaat
      181 tgcgcgccgg tcgccggcgc gatcgcgccg accttgccgc gccaggtccg gccggggaac
      241 gtgtcgaccg agatcgtcac cggcaggccg ggccggacat aggtcagatc ggattccttc
      301 gggttggcgt cgatccaaag atcgcgatca ttgacgatcg agaagaccgg cgtgccggcg
      361 gcgatgacgc ggccgagctg gatttgcggc acctgcgtcg cgacgccggg gatcggcgcg
      421 accacttcgg tgttgcgcaa gttgcgctcg gcgtcctcga cttgcgcctt ggcctcgata
      481 taggcgggaa attcttcaag cggagcgtcg ggcttgccgc cgagctggat cgccgccgac
      541 tgctgctgct gcatcacgaa agcgaggatt tgctgcgctt ggatgagggc ggcgttcgaa
      601 gtatccttgt cgttctgcgt cccggcgccg gacttcgcca gttgcgactt gcggtcgaaa
      661 tcggcctgac ggacgcgcac cgcctcctcg ctcatcttca cctggtcggc gttggctttg
      721 aacgaaagct gcagattggc gtattgattt ttcgccgcct cgagccgcgc cttggcgagg
      781 gcgagggcgg tgcggtacgg cgcggggtcg atctcgaaga gcttgtcgcc ggccttgacg
      841 tgctggcctt cggcgacatc gacatggacg atcgggccgg tcacttgcgg cgtgatcagg
      901 accttgtcgg cgccgatata agcgttgtcg gtcgagacgt agcggccgct cgacagccac
      961 cagtaaaagc cgccggccaa ggccagcgcc ggcacgacga tcagcagcac gaaacgcgag
     1021 aagccgcgcc gacgccgttt cggcgcctcg gcccgctgcg gggcgggccg ctgcgccggc
     1081 gccaaaggcg gcgccgcgtc gggcgcaggg gtggggaccg gcgcgggttg cgccggcgtg
     1141 gcgtcgtcct tgcgatcgcg cgggaaaggc ttgacttgcg acactgtgga tcaacccctg
     1201 cagttcaatt cacgtttcaa attggtcttg atgcggtcca tgcttttgcc gagcgcggcg
     1261 atttcgaagg cgtcgaaccc gttcagcgcc tggccgacca gcgcctcgcc gagttcgttc
     1321 aagcgctcca gcaccggcgc ggcgccctcg gtcaaataaa gccgattggc gcggcggtcg
     1381 ttggggtcgt cgcggcgctc gaccaggccg aggccggcta gcttgtcgac cagccgggcg
     1441 agggtgatcg gctgaaggtc gagcagttcg gcaagctcgg cttgtttgac gccctggctc
     1501 atctccaggc gcttgagcac cgaccattgc gctctggtca tattgagcgc gcgggcgcgc
     1561 tgatcggcga gggtgcgcag aagccgggcg acgtcgtgca tcgcgaaaac gaattcgcgc
     1621 gccgggctca tcggcttttt cgcgaccgcg atttgcgggg cgggcgctaa aagggaagtg
     1681 tgcggcatgg aggactgggt ctttagtgag gggtagctgg cttaatataa gctaccttat
     1741 aatatgcggc aacgcagaag agccttgcgc gagccgcatg accaaaatgc ggagggcggc
     1801 atcagcaacg gggcgaagat tccaccgcga tgcggtggcc gcaattccca tccggtcgat
     1861 cggtgaagaa accgcttgtt ggaaagcgcg atctctaatc gccgacccag ccggcgcttc
     1921 cggcgggcgc ggcaaggagc gcgcttgcga tcgcctccca ttcggccgcc agagcgtcgc
     1981 ggcagaaggg ccggccggcg cggcgatacc aataggcggc gttcgaggcg tcgccctgct
     2041 tacggtgcaa ataggcgtgg acccagtcgc cgtcgccgcc ttcgtcgcgc tgcgccaggt
     2101 catgggcggc gtcgaaatcg ccgcgcccgt cccaccacag cgcctgcagc gcgacgctta
     2161 agcccgccgg cggggcaggg cggtccaggg aggcgcggaa ggcggcgaga tccatgttct
     2221 ttcaccaggc ggcgggcggc tcgccgggcg cgctcctaac cctcccttaa ccttaacaca
     2281 tcctaatggc gttcaggcgc aggaaggctc ttctcgcgcc gcctttgcgt tgacgcccat
     2341 agtttcccat ggtatcccaa gccatcccgt cgaggttttc tgcgccggcg cggccggcgg
     2401 cgcttattgc gtcgccgccg gttattgccg cgcggctctg gacgaaggtc gttggaacgc
     2461 tttgtctccc atttcaccaa tcgcttcgac gccaagggac gaatctcgat tccggcccct
     2521 ttccgcgccg tcctggcgcg cgacggtttc gagggtcttt acctccatcc gtccttggac
     2581 gcgccggctc tcgattgcgg gggcaacgcc ttgctgagcg agatcgacgc gctcctggcg
     2641 acgatgccgg cttattcgga gccgcgcgac atgctggcga cggccttgct tggcacgagc
     2701 gaaatcctcc gaccggactc ggagggccgg gtgatcctca gcgaggcgag ccggaccttc
     2761 gcgcatttga ccgacgaagc ggttttcgtc gggcaggggc acaagtttca aatctgggcg
     2821 ccggaccgtt tccgggcgca tctcgaagag gccaagactc gagtgcgcca attgcgggcg
     2881 gagctcggcg ccagagcggc gcggagcgcg ccggcggagc gcgcggaatg atggcgagcc
     2941 gcggcgaggc gccccctccc gcaggcggac cggctcgcca cattcccgcg cttctcgatg
     3001 aagcggtcgc cgccctcggc gttcacgagg gcggcgtcta ttgcgacggc acgttcggcg
     3061 ccggcggcta tgcccgggcc attctcgatc gcggcggccg cgtcgtcgcc atcgaccgcg
     3121 atcccgaagc gatcgcggcg ggtcaggcgc tcgccgcttc ggccggcggc cggctcgcgc
     3181 tcgtcgaggg gcgcttcggc gagctcgacg cgatcgcccg cagccaaggc ttttcggcgc
     3241 tcgacggcgt cgtcctcgat atcggcgtgt cgtcgatgca gcttgacgag gcgcagcgcg
     3301 gcttctcctt tcgcaacgac gcgccgctcg acatgcgcat gggaaggagc ggccgcagcg
     3361 ccgccgacct gctcgccgaa gaagacgagc aggcgctcgc cgatatcctc tatcatttcg
     3421 gcgaggagcg cgccgcgcgc cgcatcgcgc gggcgatcgt ccacgatcgc gaagccgcgc
     3481 ctttcgtctc gacgcggcag ctcgcgtcgc tgattgagcg cgtcgcgccg gcgcggcccg
     3541 gcgaggcgat ccatccggcg acgcgcagct tccaggcttt gcgcatcgcg gtcaacgacg
     3601 aactcggcga attggcgcgc gggctcgtcg ccgccgaggc gatcctcaag cctggcggcc
     3661 ggcttgccgt tgtgaccttc cactcgctcg aagaccggat cgtcaagcaa ttcttcgcgc
     3721 gccgctccgg ccgcgggcag gcggcggggc gccgcctgcc cggcgagaag ccggctccgg
//
LOCUS       DATGJW010000305        14237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_990376, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000305 DATGJW010000000
VERSION     DATGJW010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 14237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14237
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_990376"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..656)
                     /locus_tag="VKU03_09250"
     CDS             complement(<1..656)
                     /locus_tag="VKU03_09250"
                     /inference="COORDINATES: protein motif:HMM:NF024857.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71491.1"
                     /translation="MPDAPSIISEKNGETVRLALAGGWTVETGRAAERQAEDLVGSAA
                     GAKRATIDLSGVEQLDTAGAWLIDRAQAQLTASGAAVVYSLARPEHKILLQEAHYRAF
                     DSAPRPQPHPVLEILSDIGETVSGAGRDLVAGLSFLGRLVAAIAAGIADPRRLRITPL
                     VFHLEAFGFRSVPIIVLINFLVGAIVAQQGIYQLQRFGAVVYAVDLIGILILRELGVL
                     L"
     gene            complement(855..1268)
                     /locus_tag="VKU03_09255"
     CDS             complement(855..1268)
                     /locus_tag="VKU03_09255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003527983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ71492.1"
                     /translation="MIFVDTNVVSETLKKSPDPDVLAWLVRFDAELALPTVTIAEISY
                     GIERIRPDQRSLRLEAGLAEWRRRFADRIYGLTEDAALIYGAIMGAAARTGRLMAAPD
                     GMIAAIAKANGGRLATRNRADFERTGLELISPWDF"
     gene            complement(1265..1513)
                     /locus_tag="VKU03_09260"
     CDS             complement(1265..1513)
                     /locus_tag="VKU03_09260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010023905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="plasmid stabilization protein"
                     /protein_id="HLJ71493.1"
                     /translation="MTEPQLSVRSARARDLAHRLARREKRSIADIVERALESYEVRVA
                     GREPAAAFYARLAANGVDDLDLEAVIKAVRQPHAGPHL"
     gene            complement(1697..2887)
                     /locus_tag="VKU03_09265"
     CDS             complement(1697..2887)
                     /locus_tag="VKU03_09265"
                     /inference="COORDINATES: protein motif:HMM:NF037955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ71494.1"
                     /translation="MTVIAAARREKAGLRLALVYAALFFEMGVQIPFLPVWLGARGLR
                     DGEIAIALAAPLALRVVTTPFFSGLADRRRDVPGLLSAAAMVLASACAMLGFLHGFAP
                     LLVAVAVLLCAQGLVMPLADALASLVLRAADRQGGARLDYGRIRKWGSAAFIAGNLAG
                     GLYLSVVPIEGATIALTVAALLGVAATFYAAPLGQGLKEEHAEAAEIAAGPGLGLLPM
                     VIAAAALIQTSHALLNTFGSIHWAREGHSTNFVGAAWAIGVVCETTVFGLAGRWFSGP
                     DRALALLALGGGAATLRWLIMASDPNAALLLLAQAGHGLTFACTHLGSMLFIFAAAPP
                     HMRARAQGWLTASISGLSALLIALCGPLYAHFGEVAYLAMAALAAAGLALAISVALRG
                     RKRP"
     gene            complement(2884..3501)
                     /locus_tag="VKU03_09270"
     CDS             complement(2884..3501)
                     /locus_tag="VKU03_09270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HLJ71495.1"
                     /translation="MGVEGKDKAKALEAGAAALSGQLGRTVQRLRKTYNLSLSDLSTQ
                     SGVAKSIISQIERNETNPTLATLWRLAHALDVSIESMLQGGQEGPFIEKAGRGDTPIL
                     VSDDGLCRLAVIGWLKTVDWLQWYDFQAEPGGVLESEAHQRGSIECLSLIDGELEVEV
                     GDAKETARAGETLRYRCDRPHRIRNASAKPAHATMVCILKAAVME"
     gene            complement(3752..4141)
                     /locus_tag="VKU03_09275"
     CDS             complement(3752..4141)
                     /locus_tag="VKU03_09275"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ71496.1"
                     /translation="MKALRFDHISLHVHDLAASARFYAEVLGLPEIENKTYRSDIRWF
                     GFDGHRAVHLISGGVDPPPERPLSSHYALASDAFDDAVAFLKSRKIEFVNSKGKAGEI
                     GFRADGVRQIYFRDPDNYWIEINEAQD"
     gene            complement(4234..5013)
                     /locus_tag="VKU03_09280"
     CDS             complement(4234..5013)
                     /locus_tag="VKU03_09280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010660047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ71497.1"
                     /translation="MDLGIKGRKAVICASSKGLGKACALSLARAGCDVVINGRDAKAL
                     EAAADHIRAATGAKVVAVAGDVGQPEGRKALLAACPAPDILVNNNGGPPPKPFKALSR
                     EDILAGIEANMLTPIGLVQAVVFGMAERGFGRIVNITSSSVRAPIPGLDVSSGARAGL
                     TAFLAAIARQVAADNVTINSIQPGAFDTDRLRGGMAKQAEMRGVSAEQARAEREASIP
                     AKRFGRPEEFGELCAYLASAQAGYITGQNILIDGGAFPGAF"
     gene            complement(5163..5528)
                     /locus_tag="VKU03_09285"
     CDS             complement(5163..5528)
                     /locus_tag="VKU03_09285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71498.1"
                     /translation="MIRRAFDFSCKSAVALAAISFAVAVSAPAQAQTRYPHHRHHHYA
                     RVAPAPVGGGDIVVRTGRPVFYAVDPYQEFLAGPRYYVDTALDTDPGLVHPFGRFGQS
                     VLPSRFDPPGQSAPLFTFW"
     gene            5756..6988
                     /locus_tag="VKU03_09290"
     CDS             5756..6988
                     /locus_tag="VKU03_09290"
                     /inference="COORDINATES: protein motif:HMM:NF017773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ71499.1"
                     /translation="MPQPARLSLKSLRPTMRAVAALAFAALLGGCVESTLDGAADYVG
                     SVGGLIDHKPDPKPRVVQIFIASTRKGENGAAARQLAPGGMRYALTTLTFPPGHKPGA
                     IERPMWGAGDPQKDIMVAGRRELDPDEFKAEFASHISGRIGVNRDVLIFVHGFNTPFA
                     EARWRAAQIVADSHFGGVPILFTWPTRGGLLSYASDKDSAMASRDELQALLEEVSKTP
                     GVGKIHILAHSMGAWLAMEALRQASIANDRDLGGHLGDVMLASPDIDLDVFGQQMARI
                     RPAKVTVFATSNDRALSLSSWIADDRPRVGAVNAAKPEDRAALEKLGVKVYDLSAFSD
                     GFIDHGIYADTPDVLHAIGAQLTAPRPADADKVSIIDARGDAGPDPAPAPAAPPAAPA
                     PERGAVQVTPLAPAPSAR"
     gene            complement(7080..7301)
                     /locus_tag="VKU03_09295"
     CDS             complement(7080..7301)
                     /locus_tag="VKU03_09295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71500.1"
                     /translation="MIRIVVAIGLVLLTTDLSFAQSYCEQVRQGIAQYGYAAARRYAV
                     EHYSPQEVRAADRCIAKMRGGYHRRRGYR"
     gene            complement(7438..8388)
                     /locus_tag="VKU03_09300"
     CDS             complement(7438..8388)
                     /locus_tag="VKU03_09300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ71501.1"
                     /translation="MQYRRLGRTGLQIAPLVLGGNVFGWTADKARSFELLDRFVEAGF
                     NAIDTADVYSAWAPGNKGGESETIIGEWIKSRGARGRVIVITKVGSPMGPGKKGLSPK
                     YIEEACEASLKRLGIEAIDLYLSHWPDPDTPYEQTLAGYAKLLEKGKVRFIGASNLNA
                     EQLRAALAAARRNNLPRYEALQPEYNLYDRGSFDGPLRELCMAEEIGVITYFSLAKGF
                     LSGKYRSEADLGQSPRGGGVKAYLNERGRRILHALDEVSRRLSAKPAEVALAWLMARP
                     GVTAPIASATNLDQLESLIKAASLSLAPAEMKALDEASAL"
     gene            8567..8992
                     /locus_tag="VKU03_09305"
     CDS             8567..8992
                     /locus_tag="VKU03_09305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLJ71502.1"
                     /translation="MPIDALKVGFRRENSIVVDEGLTVPSVSRAFKGFAEMPPVFATA
                     FLVGFVEWTCVEALRPFLSPDQRTVGVHVDLSHSAATPVGMRVFAAVELTAIEGRKLR
                     FKVVCRDEAEVICEGSHDRYIIDEPRFLARLAKKRESLK"
     gene            9287..11863
                     /locus_tag="VKU03_09310"
     CDS             9287..11863
                     /locus_tag="VKU03_09310"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HLJ71503.1"
                     /translation="MGARRLPGILSRAFVAALTLIVVAALAAPAFAAGRRVALVIGQA
                     AYESLPRLENTTNDAEAVKGAMQKAGFEVYFGVDLKRVPFEDLLKRFYRAADGAEIAL
                     LYYSGHGVQVGGANYIVPVDAQLATAYDIELQTINVDDIFEYLNAHTSAQLIFLDACR
                     TNPFRIQKYWIADTLKPIGQTTGLARSAANIGTLIAFSTEPGKIAYDGTGPTSPYTTA
                     FVRHIATPNQEIRQALTQIRRDVIAATGGRQVPWENSSLAEDLYLVRAPSPPAVPPMV
                     RIDAPDPTRPIPLHLPEPLAETGGPLKVQIDHLPEQGRLLLNGKPLDKTAALTGADLD
                     KLSYDPAGAPRGAVGLMSYVVSDSYNQATRGVVAITIGAEKSPDALEQREARRSAAAQ
                     AVEAYFKSLDHFAATPPVGVGPTPLGLAGAPAEAAEIGAQVAVAATPQDGALLLGQRT
                     LTPGARMALADLPKLAFEPKVGADSHSDAISLKSLFEPQVQIALSVTPKLDACDSAAA
                     APLDLQGVAPGKAPNEIDAPAAIAACKRALEAHPGVARFAYQLGRAQLAAGLADEARR
                     SFQEADDKKHMRAVAALGDMALFGVFGAADPAKADAYYKACAAGGDAYCLHSYGKALF
                     YGQGVAKDVSRGLKLMIRAAELGHTYAMNELGYIFTYGRNVPVDVERGIRYYEAGAAR
                     GDIYSYNNLGLVYLRGKGRPVDYAKARDYFLKASDGGQPYAPTNIGVMYRDGDGVPAD
                     LDAAAKWLELGAKRGDYWGALYRARLNADRPGAAVDAAIWLARAAALDVDRGNADPDH
                     QARRMLAKLGEADKRQALARLEAELGPDESAKVTARALDDRLAEVSNRLWRRNKPRYD
                     LF"
     gene            11902..12504
                     /locus_tag="VKU03_09315"
     CDS             11902..12504
                     /locus_tag="VKU03_09315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007628121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098024 - virus tail, fiber [Evidence
                     IEA];
                     GO_process: GO:0019062 - virion attachment to host cell
                     [Evidence IEA];
                     GO_process: GO:0046718 - viral entry into host cell
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tail fiber domain-containing protein"
                     /protein_id="HLJ71504.1"
                     /translation="MIGGKRLRQAALYGGLFIAAAMIAAGGAAAAMEAKHAPLHHHYY
                     HHHHRHYHRHHARAAKPRHVAKHRARPHTNAAAAAPAVSPGPAASDPPKTSTGAIGGG
                     GGGGGGGGSGGGSWSDRRLKRDIRRIGTSPSGLRIYAFRYVWGGPVFVGVMAQDLLES
                     RPDAVIETESGLLMVDYDRIDVKMTTLDERLAVQRVQDAA"
     gene            complement(12534..13865)
                     /locus_tag="VKU03_09320"
     CDS             complement(12534..13865)
                     /locus_tag="VKU03_09320"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: protein motif:HMM:NF006055.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HLJ71505.1"
                     /translation="MRLWIKDPLAIFAHRAERGLVVEGTKIVEIVGEGSAPASPVDET
                     FDASCHVVLPGLINTHHHFYQTLTRAHPQAINKELFPWLTALYPIWARLRPEHLRLAT
                     RLALTELMLSGCTTAADHHYLFPRGLENAIDIEVEEARALGLRMTVTRGSMNRSQKDG
                     GLPPDEVVQSEEEILSDSERVLKLFHDPKPGAQIRIALAPCSPFSITKALMRDTAALA
                     EQYDCQLHTHLCETKDEEKFCLEMYGVRPVELLEETGWMSKRAWLAHGIHFNAEEIAA
                     LGKAGVGICHCAASNMVLASGICRTKELEAAGAALGLGVDGSASNDSSNMMEALRHAL
                     MIGRLRYGASGVTHHDVLRWATEGSARCLGRADIGRIEAGLEADLALFTLDEPRFSGA
                     HDPLAALILCGAHRADRVMVAGSWRVIDGAPIGVDLAGLIAAHRAAAAAFS"
BASE COUNT         2529 a         4766 c         4648 g         2294 t
ORIGIN      
        1 agaagaacgc cgagctcgcg caggatcaaa atgccgatca ggtcgaccgc atagacgacc
       61 gcgccgaagc gctgcaactg gtagatgccc tgctgcgcga cgatagcgcc gaccaaaaaa
      121 ttgatcagca cgatgatcgg cacgctgcgg aagccgaacg cctccagatg aaagacgagc
      181 ggcgtgatcc gcaagcggcg cggatcggcg attcccgccg cgatcgccgc gacgaggcgg
      241 ccgagaaagc tgaggccggc gacgaggtcg cgcccggcgc cgctgacggt ttcgccgata
      301 tcggagagga tttccagaac cggatgcggc tgcggccgcg gggccgagtc gaaggcgcgg
      361 taatgggctt cctgaagaag gattttatgc tcgggccgcg ctagcgaata gacgacggcg
      421 gcgcccgagg cggtcaattg cgcctgggcg cgatcgatca gccaggcgcc ggccgtgtcg
      481 agctgctcga cgccgctcag atcgattgtc gcgcgcttcg cgccggccgc cgagccgacc
      541 aggtcctcag cctggcgctc ggccgccctc cccgtctcga ccgtccagcc gccggccagc
      601 gccagtcgaa cggtctcgcc gtttttttcg ctgatgatcg aaggcgcgtc aggcatttag
      661 gcgctcggag ggtcgccggc tcttatcgcg ctcttaagag cctgtcgagg ttgacgaaac
      721 cttgagccat ggcgcagcgc cgacgcgccg aaacgggatt tcccggccag aacgcggaca
      781 aaaccggcac gaatcggcgt tgcagcgatt cgcgcgctga agtcaagcgc gctctgcgca
      841 aggtctgcgc aaggtcaaaa atcccaagga gagatcagtt cgaggccagt ccgttcgaaa
      901 tcggcgcgat tgcgtgtcgc gagccgcccg ccattggcct ttgcgattgc ggcgatcatc
      961 ccgtcggggg ccgccatgag tcgcccggtc cgcgctgcag cccccatgat cgccccatag
     1021 atcagggctg cgtcttcggt caggccgtag atccgatcgg cgaagcgccg ccgccattcc
     1081 gcgagccccg cctccagcct caaggagcgt tgatcggggc gaatcctttc aatgccgtag
     1141 gaaatttcgg caatcgttac ggtcggcagc gcgagttccg cgtcgaaccg gacgagccaa
     1201 gccaaaacgt cgggatcggg cgatttcttg agggtttcgg agacgacgtt cgtatcgacg
     1261 aagatcatag atgcggaccg gcatgaggct gccgcaccgc cttgatcacc gcctcaagat
     1321 caagatcgtc gacgccgttg gcggcgagcc gcgcgtaaaa ggcggcggcc ggctcccgac
     1381 cagcgacgcg aacttcatag gattcgagcg ctcgctcgac aatgtcggca atcgatcgtt
     1441 tctcgcggcg ggcgagacga tgcgcaagat cgcgcgcgcg ggcgctgcga accgacaatt
     1501 gcggctcagt catgagcaaa gacccttctc tctcataaga ccccatatgc attgctcagc
     1561 gtctgccatc aagatggcta cagacggcaa aggcgatcgc tcgagggccg gcgacaaaaa
     1621 tcgctccgca attcgcgcct gtgaaatatg ccaaggcgaa accgtggcgg tcgccgaatg
     1681 acggcgatcc atccgctcag gggcgctttc ggccgcgcag cgcgacggaa attgcgagcg
     1741 ccagccccgc cgccgccagc gcggccatcg ccagatacgc cacttcgccg aaatgggcgt
     1801 agagcggccc gcatagcgcg atcagcaagg cgctcagccc ggaaatcgag gcggtgagcc
     1861 agccttgcgc ccgcgcccgc atatgcggcg gcgccgcggc gaagatgaac agcatcgagc
     1921 cgagatgggt gcaggcgaag gtgaggccgt ggccggcctg ggcgaggaga agcaaagcgg
     1981 cattcggatc gctcgccatg atcagccagc gcaacgtggc ggcgccgccg ccaagcgcca
     2041 gcaaggcgag ggcgcggtcc ggcccggaga accagcggcc ggcgaggccg aagaccgtgg
     2101 tctcgcagac cacgccgatc gcccaggcgg cgccgacgaa attggtcgaa tgaccctcgc
     2161 gcgcccagtg aatcgagccg aacgtgttga gcaaggcgtg gctcgtctga atcagcgcgg
     2221 cggcggcgat gaccatcggc agcaaaccga ggcccgggcc ggccgcaatc tcggccgcct
     2281 cggcatgctc ctctttcaag ccctggccga gcggcgccgc atagaaggtc gcggcgacgc
     2341 cgagcaaagc cgcgacggtg agcgcgatcg tcgcgccttc gatcggcacg acgctcaaat
     2401 agaggccgcc ggcgagattg ccggcgatga aggcggccga gccccatttg cggatgcggc
     2461 cgtaatcgag cctggcgccg ccttgccgat cggcggcgcg caggacgagc gaggcgaggg
     2521 cgtcggcgag cggcatgacc agcccttgcg cgcagagaag taccgcgacg gcgaccaaga
     2581 gcggcgcaaa gccgtgcaga aaaccgagca tcgcgcaggc gctggcgaga accatcgccg
     2641 ccgccgagag caggcccggc acatcgcgcc ggcgatcggc gaggccggag aagaacggcg
     2701 tcgtcacgac tctcagcgcc aacggcgcgg cgagcgcgat ggcgatctcg ccgtcgcgca
     2761 agccgcgcgc gcccagccac accggcagga acgggatctg cacgcccatt tcgaagaaca
     2821 aggcggcgta gaccaaagcg aggcgcagtc cggctttctc gcgccgcgcc gcggcgatga
     2881 cggtcactcc atcaccgccg ctttcagaat gcagaccatc gtcgcatggg cgggcttggc
     2941 cgaagcgttg cggatgcgat gcggccggtc gcagcgatag cgcaacgtct cgccggcccg
     3001 cgccgtctcc ttggcgtcgc cgacctcgac ttccaattcg ccgtcgatca gcgacaggca
     3061 ttcgatcgaa ccgcgctgat gggcttcgga ttcgagcacg ccgccgggct cggcctggaa
     3121 atcgtaccat tgcagccaat cgacggtctt cagccagccg atcacggcga gccggcagag
     3181 cccgtcgtcg gaaaccagga tcggcgtatc gccgcggccg gctttttcga tgaacgggcc
     3241 ttcctgcccg ccctgcagca tgctttcgat cgagacgtcg agcgcatggg cgaggcgcca
     3301 cagcgtcgcg agggtcggat tggtctcgtt gcgctcgatc tgcgagataa tcgatttggc
     3361 gacgcccgac tgggtcgaga gatcggacaa cgacaggtta taagtcttgc gcagccgctg
     3421 caccgtgcgc ccgagttggc cggacagcgc cgccgcgccc gcctccagcg ccttcgcctt
     3481 gtccttgcct tcgacgccca tcgcccgaat ccagagcctt cccgtttgac ataatagacg
     3541 ggttcgtccg gtttatcgaa cgattttttc gccgcagcgg cctctttgtc attcccgagg
     3601 aagcgggaaa atcagaaacg gccgcatcgg aaaggcgggc cagtctgggc gacgattttg
     3661 aggcgaatca aaaatcgtcg cgaagttttc tcatggcctg tttcgcggga atgacgctgg
     3721 aaacgcccgc gaaacagcga ccgagaagag ctcaatcctg cgcctcgttg atctcgatcc
     3781 aataattgtc ggggtcccgg aaatagattt gccgcacgcc gtcggcgcga aagccgatct
     3841 cgcccgcctt tcccttggaa ttcacaaatt cgatcttgcg gcttttgaga aacgcgaccg
     3901 catcgtcgaa cgcgtcgctg gctagcgcgt aatgcgagga gagcggccgc tccggcggcg
     3961 gatcgacgcc gccgctgatc aggtgaaccg cgcgatgacc atcgaagcca aaccagcgaa
     4021 tgtcgctccg gtacgtcttg ttctcgattt caggcaagcc gagaacctcg gcgtaaaagc
     4081 gcgcgcttgc ggcgagatcg tggacgtgaa gcgaaatatg atcgaagcga agcgccttca
     4141 tgggcgtgcc tttcggacat aaaacggcgc attcttcgct cgcgccgcga agcggtcaaa
     4201 taaagccccg gtcattcccg ggaatgacag ggcttagaac gcgcccggaa acgcgccgcc
     4261 gtcgatgagt atgttctgcc cggttatgta ccccgcttga gcgctggcga gataggcgca
     4321 gagttcgccg aactcctccg gccggccgaa gcgcttggcc ggaatcgagg cttcgcgctc
     4381 ggcgcgcgcc tgctcggcgc tgacgccgcg catctccgcc tgcttggcca tgccgccgcg
     4441 caggcgatcg gtgtcgaagg cgcccggctg gatcgaattg atcgtcacat tgtccgccgc
     4501 gacctgccgc gcgatggcgg cgagaaaggc ggtgagcccg gcgcgggcgc cagacgagac
     4561 gtcgaggccc ggaatgggcg cgcgcaccga agacgaggtg atattgacga tgcggccgaa
     4621 cccgcgttcg gccatgccga aaacgaccgc ctgaacgagc ccgatcggcg tcagcatatt
     4681 ggcctcgatc ccggcgagaa tgtcctcgcg gctcagcgct ttgaaaggct tgggcggcgg
     4741 tccgccattg ttgttgacga gaatgtccgg cgccggacag gcggcgagca gcgcctttcg
     4801 tccttccggt tggccgacat cgccggcgac ggcgaccact ttggcgccgg tcgcggcgcg
     4861 gatgtgatcg gcggccgctt cgagcgcctt ggcgtcgcgg ccattgatca cgacgtcgca
     4921 gccggcgcgg gcgagcgaaa gggcgcacgc cttgccgagg cccttgctcg atgcgcaaat
     4981 gacggctttg cggcccttta tgccgagatc catatccgct ccgcgggaaa ttgcgacgag
     5041 accgcagctt tacaccgcgg ggcgcggcgt ttgaactcgg aaaatcctgc tcgcgcgcgg
     5101 tcttcagcgc cgctcgcgcg tcgcaatcga ggacaacgcg ccttgcgagg cgccggggac
     5161 gatcaccaga aggtgaaaag cggcgcgctc tgaccgggcg ggtcgaagcg cgacggcaac
     5221 accgactggc caaaacgccc aaacggatgg acgaggcccg gatcggtatc gagcgccgta
     5281 tcgacgtagt agcgcggccc tgcgaggaat tcctggtaag gatcgacggc gtagaaaacc
     5341 gggcgacccg tgcgcacgac aatgtccccg ccgccgacgg gcgcaggcgc gacgcgcgca
     5401 taatggtgat ggcgatgatg cgggtagcgg gtttgcgctt gcgcaggggc gctcaccgcg
     5461 accgcgaagc tgattgccgc gagcgcaacg gcgctcttgc aggaaaaatc gaatgcgcgc
     5521 cgaatcatga acgcctcctt ggcggggcct gtccgctcat atgggggcga aaagcctctc
     5581 agaacaagct taacgccctt tcgtcaccgt taattgaatg cgaccgcgcg gcaaaggttc
     5641 cgcccgtcgg gcagtccggc ccaaccgctt agtcctattc tcgccacatg gctgacccat
     5701 gagccgcgca agaatccgcc atgggcggat cgctcaaagg ggaaagtcgc gccgcatgcc
     5761 ccagcccgcg aggctcagcc tgaaatctct gcggccgaca atgcgggcgg tcgccgcctt
     5821 ggctttcgcc gcgttgctcg gcggctgcgt cgagagcacg cttgacggag cggccgatta
     5881 tgtcggctcg gtcggcggcc tgatcgatca caagcccgat ccgaagccgc gcgtggtgca
     5941 gattttcatc gcctcgaccc gcaagggcga gaacggcgcc gccgcccggc aactcgcccc
     6001 gggcggaatg cgctatgcgc tcaccaccct cacctttccg ccgggccata agccgggcgc
     6061 gatcgagcgg ccgatgtggg gcgcgggcga cccgcaaaag gacatcatgg tcgccggccg
     6121 ccgcgagctc gatcccgacg agttcaaggc cgaattcgcc tcccatattt cgggccgcat
     6181 cggcgtcaac cgcgacgtcc tgatcttcgt ccacggcttc aacacgccct tcgccgaggc
     6241 gcgctggcgc gccgcccaga tcgtcgccga cagccatttc ggcggcgtgc cgatcctgtt
     6301 cacctggccc acgcgcggcg gcctgctgag ctacgcctcg gacaaggaca gcgccatggc
     6361 ctcgcgcgac gaattgcagg cgctgctcga agaagtctcc aagacgccgg gcgtcggaaa
     6421 aatccacatc ctcgcccatt cgatgggcgc ctggctggcg atggaggcgc tgcggcaggc
     6481 gtcgatcgcc aacgatcgcg accttggcgg ccatctcggc gacgtgatgc tggcctcgcc
     6541 cgatatcgac cttgacgttt tcggccagca aatggcgcgc atccggccgg ccaaggtcac
     6601 ggtgtttgcg acgtcgaacg atcgcgcctt gtcgctgtca agctggatcg ccgacgaccg
     6661 gccgcgcgtc ggcgccgtca acgccgccaa gccggaagac cgggcggcgt tggagaaact
     6721 cggcgtcaag gtctacgacc tttccgcctt ttccgacggc ttcatcgatc acggcatcta
     6781 tgccgacacg ccggacgtcc tgcatgcgat cggcgcgcaa ctgaccgcgc cgcggcccgc
     6841 cgacgccgac aaggtctcga tcatcgacgc gcgcggcgac gccgggccag atccggcgcc
     6901 tgccccagcc gcgccgcccg ccgcgcctgc gcctgagcgc ggcgccgtgc aggtgacgcc
     6961 gctggcgccg gcgccgtccg cgcgctgacc tttgcgccat ccttcgcgtc cttcgtgttt
     7021 aatttttcaa cacgacggac gccaagacat tcaccaagac cgccgacgcg ctgcgcgcgt
     7081 taacggtaac cgcgtcggcg atgatagccg ccgcgcatct tggcgatgca gcggtccgcg
     7141 gctctcactt cctggggact gtaatgctcg acggcgtagc gccgggccgc ggcatagccg
     7201 tattgggcga tcccttggcg tacttgctcg caataggatt gcgcaaagga gagatcggtc
     7261 gtcagcagga caaggccgat ggcgacgaca attctgatca cggcgtttct ctcctctgtt
     7321 cgccaggccg actccgattt cggcttgcgc cccggtcctc ccaataatcg ttttgccccg
     7381 gtttttgttt ccccgcgcca aagagacagc gattccgaat ttccggaccc agcccgccta
     7441 gagcgcgctc gcctcatcaa gcgccttcat ctccgccggc gctaggctca gagacgccgc
     7501 cttgatcagg ctttcgagct ggtcgagatt ggtggcgctg gcgatcggcg ccgtgacgcc
     7561 gggccgcgcc atcagccagg ccagggcgac ttcggcgggc ttcgccgaaa gccgccgcga
     7621 gacttcgtcg agggcgtgca ggatgcgccg gccgcgctcg ttgagatagg ctttcacgcc
     7681 gccgccgcgc gggctttggc cgagatcggc ctcgctgcga tatttgccgg agagaaagcc
     7741 cttggccagg ctgaaatagg tgatgacgcc gatctcttcg gccatgcaaa gctcgcgcaa
     7801 cggcccgtcg aaagagccgc ggtcgtagag attgtattcc ggctgcagcg cttcgtaccg
     7861 cggcaggttg ttgcgccggg cggcggcgag cgcggcgcgg agctgctcgg cgttgagatt
     7921 ggacgcgccg atgaaccgta ctttgccctt ctcgagaagc ttggcgtagc cggcgagcgt
     7981 ctgctcgtaa ggcgtgtccg gatcgggcca gtgcgacaga taaagatcga tcgcctcgat
     8041 cccgagccgc ttcagcgagg cctcgcacgc ctcctcgata tatttcggcg acaggccttt
     8101 cttgcccggc cccatcggcg agccgacctt ggtgatgacg atcacgcggc cgcgcgcgcc
     8161 ccgcgatttg atccattcgc cgatgatcgt ctccgattcg ccgcctttat tgccgggcgc
     8221 ccaagccgaa tagacatcgg cggtgtcgat cgcgttgaac cccgcctcga cgaaccggtc
     8281 gagcagttcg aacgagcgcg ccttgtcggc cgtccagccg aaaacattgc cgcccaggac
     8341 gagcggcgcg atctgaaggc cggtgcggcc gaggcgacgg tattgcatgc gcagctccaa
     8401 aatgtggtaa gcccggcgag cgagctaaaa accgcatgct agcagccgcg cgccggcggc
     8461 ggaaagcatc gagcgttgac atttgcgtca atgacgcagg atcgacgcga attaggccga
     8521 gaacgcccgg tttccggacc ccaaaatgga ttgcattgga gcagccatgc cgatcgatgc
     8581 gctaaaagtc ggatttcggc gcgaaaactc gatcgtcgtc gacgaagggc tcaccgttcc
     8641 gtcggtttcg cgggcgttca agggctttgc cgagatgccg ccggtcttcg ccaccgcctt
     8701 cctggtcggc ttcgtcgaat ggacctgcgt cgaggcgctg cgcccgttcc tgtcgcccga
     8761 ccagcgcacg gtcggcgtcc atgtcgacct cagccatagc gccgcgacgc cggtcgggat
     8821 gcgcgtcttc gccgcggtgg aattgacggc gatcgaaggg cgaaaattgc gattcaaggt
     8881 ggtttgccgc gatgaggcgg aggtcatctg cgagggaagc catgaccgat acatcatcga
     8941 tgaaccgcga ttcctggcgc ggctcgccaa gaagcgcgaa agtctgaaat agacggcgcg
     9001 cattcttgca tgcgcgccac gccggccgat tgccgtcaac tcgccatagc gcctatcaaa
     9061 gtacgcgcaa acgaacgccg ctcgtgcggc gcggggaggc gcgcatgttc ctttttctgc
     9121 ggttcggcgc ccagatacgc gacgagaaat ctcaaaactt catcattttc cccgcgcgat
     9181 gcggggcaat gttgactttt ccgcgaggcg cgggcgagat gtccccgacc ggaccgcccg
     9241 cagaccgtaa gagggggagc gatcgccccg ttcgacggag gtggcaatgg gggcccgccg
     9301 actgccagga attctctcgc gcgcattcgt cgccgcgtta acgctgatcg tcgtcgcggc
     9361 gttggcggcg ccggctttcg ccgcgggcag gcgcgtcgcg ctcgtcatcg gccaggccgc
     9421 ctacgaaagc ctgccgcggc tcgagaatac gaccaacgac gccgaggccg tcaaaggcgc
     9481 gatgcaaaag gcgggcttcg aagtctattt cggggtcgac ctgaaacgcg ttccgttcga
     9541 ggatctgctc aagcgcttct atcgcgccgc ggacggcgcc gagatcgccc tgctctacta
     9601 ttccggacac ggcgttcagg tcggcggggc caactacatc gtgccggtcg acgcccaact
     9661 cgcgaccgcc tatgacatcg agttgcagac gatcaatgtc gacgacattt tcgaatatct
     9721 gaacgcccat accagcgcgc agctcatttt tctcgacgcc tgccgcacca atccgttccg
     9781 catccagaaa tactggatcg ccgacacgct gaagccgatc ggccagacga cgggcctcgc
     9841 gaggtccgcc gccaatatcg gaacattgat cgccttctcc accgagcccg gcaagatcgc
     9901 ctatgacggg accggcccga ccagccccta caccaccgct ttcgtgcgcc atatcgcgac
     9961 gccgaaccag gaaatccgcc aagccctgac ccagatccgc cgcgacgtca tcgcggctac
    10021 cggcggcagg caggtgccgt gggaaaactc gtcgctcgcc gaggacctct atctggtgcg
    10081 cgcgccgtcg cctcccgcgg tcccgccgat ggtgcggatc gacgcgcccg atccgacccg
    10141 gccgatcccg ctgcatctcc cggagcccct ggccgaaacc ggcggtccgc tcaaagtgca
    10201 gatcgatcat ctgcccgagc aggggcggct tctcctgaac ggcaagccgc tcgacaagac
    10261 cgccgcgctc accggcgccg atctcgacaa gctctcctac gatcccgccg gcgcgccgcg
    10321 cggcgcggtc gggctgatga gctatgtcgt ctccgattcc tacaatcagg cgacgcgcgg
    10381 cgtcgtcgcg atcacgatcg gcgccgaaaa aagtcccgac gcgctcgaac agcgcgaggc
    10441 gcgccggagc gccgcggcgc aggccgtcga ggcttatttc aaaagcctcg accatttcgc
    10501 cgcgacgccg ccggtcggcg tcggcccgac cccgctcggc cttgccggcg cgcccgcaga
    10561 ggcggccgaa atcggcgccc aggtcgccgt cgcggcgacg ccgcaggacg gcgccttgct
    10621 gctcggtcaa cgcactctca cgcccggcgc ccgaatggcg ctcgccgatc tgccgaagct
    10681 cgccttcgag ccgaaggtcg gcgccgattc ccattcggac gcgatcagcc tcaagtcgct
    10741 ctttgagccg caagtccaga tcgccttgtc ggtgacgccg aagctcgacg cttgcgactc
    10801 cgccgcggcg gcgcctctcg atctgcaagg cgtcgcgccc ggcaaggcgc cgaacgaaat
    10861 cgacgcccct gccgcgatcg ccgcgtgcaa gcgcgcgctc gaggcccatc cgggcgttgc
    10921 gcgcttcgcc tatcagctcg gccgcgcgca gctcgccgcc ggcctcgccg acgaggcgcg
    10981 gcgctccttc caggaggccg acgacaagaa acatatgcgc gccgtcgcgg ctctcggcga
    11041 catggccctg ttcggcgttt tcggcgccgc cgatccggcc aaggccgacg cctattacaa
    11101 agcctgcgcg gcgggcggcg atgcgtactg cctgcattcg tacggcaagg cgctgttcta
    11161 cggccaggga gtcgccaagg acgtttcgcg gggcctgaag ctgatgatcc gcgcggcgga
    11221 gctcggccac acttacgcga tgaacgagct cggctacatc ttcacctatg gccgcaacgt
    11281 tcccgttgat gtcgagcgcg gcatccgcta ctacgaggcg ggcgcggcgc gcggcgacat
    11341 ctattcctac aacaatctcg gcctcgtcta tttgcgcggc aaaggccggc cggtcgacta
    11401 cgccaaggcg cgcgattatt ttctcaaagc ctctgacggc ggccagcctt acgcgccgac
    11461 caatatcggc gtcatgtacc gggacggcga cggcgttccg gccgatctcg acgccgcggc
    11521 caaatggctc gaactcggcg ccaagcgcgg cgactattgg ggcgcgctgt accgggcgcg
    11581 gctgaacgcg gatagacccg gcgcggctgt cgacgcggcg atttggctcg cgcgcgccgc
    11641 ggcgctcgac gtcgatcgcg gcaacgccga cccggatcat caggcgaggc gcatgctcgc
    11701 gaagctcggc gaggccgaca agcgtcaggc gctggcgagg ctggaggcgg agctcgggcc
    11761 ggacgaaagc gcgaaagtca cggcgcgcgc gctcgacgat cggctggccg aggtctccaa
    11821 ccggctgtgg cggcgaaaca agcctcgtta cgatctcttt tgaaacgcaa tcagcgacca
    11881 cgtattaagg gagttacagc catgatcgga gggaaaaggt tgcggcaggc ggcgctctat
    11941 ggcggtctct tcatcgccgc ggcgatgatc gccgccggcg gcgcagcagc ggcgatggag
    12001 gcgaaacacg cgccgttgca ccatcattac taccatcatc atcatcgaca ctatcatcgc
    12061 catcatgcgc gcgcggctaa gccgcgccac gtcgccaagc accgggcgcg gccgcacacg
    12121 aacgccgcgg cggcggcgcc agccgtctcg cccggtccag cggcttccga tccgccgaag
    12181 acaagcacag gcgcgatagg cggcgggggc ggcggtggtg gcggaggcgg atcaggtggc
    12241 ggaagctggt ccgatcgccg gctgaaacgc gacatccgcc gcatcgggac ttcgccgagc
    12301 ggcctccgca tctacgcctt ccgctatgtc tggggcggcc ccgtcttcgt cggcgtgatg
    12361 gcccaggatc tcttggagag ccggcccgac gccgtaattg aaacggagag cggcttactc
    12421 atggtcgatt acgacaggat cgacgtgaag atgacgacgc tcgacgagcg cctcgccgtc
    12481 cagcgcgtcc aagacgcggc ctgatcgtcc gaggagagta cggcggcgaa cgattagctg
    12541 aacgccgccg ccgcggcgcg gtgcgccgcg atcaggccgg caagatcgac gccgattggc
    12601 gcgccgtcga tcacccgcca gcttccggcg accatcaccc ggtcggcgcg gtgggcgccg
    12661 caaaggatca gcgcggcgag cgggtcatgc gcgccggaaa agcgcggctc gtcgagggtg
    12721 aagagcgcaa gatcggcttc aagcccggct tcgatgcggc caatatccgc gcgcccgagg
    12781 cagcgcgccg agccttccgt cgcccagcgc aggacatcat gatgcgtcac gcccgatgcg
    12841 ccgtagcgca ggcggccgat catcagcgcg tggcgcagcg cctccatcat gttggaggaa
    12901 tcgttcgacg ccgagccgtc gacgccgagc ccgagggccg cgccggccgc ttcgagctcc
    12961 ttggtccggc agattcccga ggcgagcacc atgttggagg ccgcgcaatg gcagattccg
    13021 acgccggctt tgccgagcgc ggcgatctcc tcggcgttga aatgaatccc atgcgccagc
    13081 caggcgcgct tgctcatcca gccggtctct tcgagcaact cgaccgggcg caccccgtac
    13141 atttcgaggc agaacttctc ttcgtccttg gtctcgcaga gatgcgtgtg caactggcaa
    13201 tcgtattgtt cggcgagcgc ggccgtatcg cgcatcagcg ccttggtgat cgagaagggc
    13261 gagcagggcg cgagcgcgat gcggatttgc gcgccgggct tcggatcgtg aaagagcttg
    13321 agcacgcgct cgctgtcgga aaggatttcc tcctcgctct gcaccacctc gtcgggcggc
    13381 aggccgccgt ccttctgcga gcgattcatc gagccgcgcg tcaccgtcat ccggaggcct
    13441 agggcgcgcg cctcttcgac ctcgatgtcg atcgcgttct ccagcccgcg cgggaaaaga
    13501 tagtgatgat cggcggccgt cgtgcagccg gacagcatca gttcggtcaa agcaagccgc
    13561 gtcgccaggc gcagatgctc aggcctgagc cgcgcccaga tcggatagag cgcggtcagc
    13621 cagggaaaca gctccttgtt gatcgcctgc ggatgggcgc gggtcagcgt ctggtagaaa
    13681 tgatgatgcg tgttgatcag gccgggcaga acgacgtgac aagacgcgtc gaatgtctca
    13741 tcgactggcg aagccggcgc gcttccttcg ccgacgatct cgacgatttt cgtcccctcg
    13801 acgaccaggc cgcgctcggc tcggtgagcg aaaatggcga gcgggtcttt gatccagagg
    13861 cgcatctggc cacattatcc cgattccgac gcggcgcgat attcccttct ccccgctccg
    13921 cgcagcgctg cccggggaaa atctgaccgt cacggcgcat gaacgatctc gcgtcttcgg
    13981 atttcctgcg cctcggcggc gtcgttgttc acaacgagct tgcgaagcag ctcagggagc
    14041 cgcacatgcc cggcgccgcc ccttatccag cgcttcgcgc cgccttctcc ccgctgcacg
    14101 cagggctgtc cggggaaagt ctgaggttat ggcgcgtctt ggacgtaatc tttcagcgcc
    14161 gcggcgccac gccggccgat cgccgtcaac ccgccatagc gccggcaagc tacgtgcaag
    14221 atcatatccg cttgtgc
//
LOCUS       DATGJW010000306         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_58717, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000306 DATGJW010000000
VERSION     DATGJW010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58717"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1102)
                     /locus_tag="VKU03_09325"
     CDS             complement(<1..1102)
                     /locus_tag="VKU03_09325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLJ71506.1"
                     /translation="MLTTLAVANYRSLRDLVVPLGRLNIVTGPNGSGKSSLYRALRLL
                     AETAKGGLIASLAREGGLPSTLWAGPEKISRSMKRGEHRVEGGPRADSTHLRLGFASE
                     DFGYAIDLGLPNADGPEKGGTAFVLDPEIKRECIWSGPFFREATKLADRRGPVLRASD
                     EDGAWRVVPQPLSSFESMMTEYADPRAAPEMIAVRERIRSWRFYDHFRTDEDAPGRRP
                     QIGTHTPVLSDSGADLAAALQTIREIGDAEGLDRAVDDAFPGAKIEIRQNDGRFESTL
                     RQPGLLRPLKAPELSDGTLRYLLLIAALLTPRPPTLFVLNEPETSLHPDLLPALARLI
                     AAAAKSSQAIVVTHSETLIAGLTRENDARAIAL"
     gene            1332..>2757
                     /locus_tag="VKU03_09330"
     CDS             1332..>2757
                     /locus_tag="VKU03_09330"
                     /inference="COORDINATES: protein motif:HMM:NF012933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AlkA N-terminal domain-containing protein"
                     /protein_id="HLJ71507.1"
                     /translation="MDLDPAQCYRALLARDARYDGLFFTCVKTTGIYCRPICPARPPK
                     FENCRFVATAAAAQEAGFRPCLRCRPESSPDLGAWRGASATVSRALALIEDGALDAGD
                     VTALAERLGVGERHLRRLFSRHLGAAPVTVAQTRRVLLAKQLIHETDLPMTEVALASG
                     FNSLRRFNETFQQLYRRPPSALRRRAGAETEESGLSLLLPYRPPYDWDSMIRFLAARA
                     LPGLEIVADQRYARVVEMRGAVGSIEIAQAPDRSALRAMVRFPRFDCLPTIVARIRRL
                     FDLGAEPIAIGAALARDPTLRPLVAARPGLRVPGAWDGFEIAVRAVLGQQITVKAATG
                     LAAKLVAVLGDPIGGGAGLPGLTHSFPRPERLDANSLAALGMPKGRAAAIAALAAAAR
                     ADPHLFDPRRDLNEAVARLRQLPGIGEWTAQYIAMRALRESDAFLVDDVAVQRALAVD
                     GRRPRGRELLARAEIWRPWRAYAVL"
BASE COUNT          411 a          968 c          920 g          458 t
ORIGIN      
        1 ccaaagcgat ggcgcgcgcg tcattctcgc gcgtcaatcc agcgatcaac gtttcgctat
       61 gcgtgacaac aatcgcctgc gaagattttg ccgcggcggc gatcaggcgg gcgagggccg
      121 gcaagagatc gggatgcagg ctcgtctcgg gctcgttgag aacgaacagc gtcggcggcc
      181 gcggcgtgag cagcgcggcg atgagcagca gataacgcag cgtgccgtcc gagagctccg
      241 gggctttcag cggccgcagc aggccgggct ggcgcaatgt cgactcgaac cgcccgtcat
      301 tctgccggat ttcgatcttt gcgccgggaa aggcgtcgtc taccgcgcgg tccagccctt
      361 cggcgtcgcc gatttcccgg atcgtttgca gcgccgctgc gaggtccgcg ccgctgtcgc
      421 tcaggaccgg cgtatgcgtg ccgatctgcg ggcggcgccc cggggcatcc tcatctgtgc
      481 ggaaatggtc gtagaagcgc caggagcgta tgcgctcgcg caccgcgatc atttccggcg
      541 cggcgcgcgg atcggcatat tcggtcatca tgctctcaaa cgacgagagc ggctgcggaa
      601 caacgcgcca ggcgccgtcc tcgtcgctgg cgcgcagaac cgggccgcgc cggtcggcca
      661 gcttcgtcgc ttctcgaaag aacggacccg accagatgca ttcgcgcttg atttccggat
      721 cgagaacgaa ggccgtgccg cccttctccg gtccgtcggc gttcggtagg ccaagatcga
      781 tggcgtagcc gaaatcctcc gaggcgaagc ccaagcgaag atgcgtcgaa tcggcgcgcg
      841 gcccgccttc gacgcgatgc tcgccgcgct tcatgctgcg gctgatcttt tccggcccgg
      901 cccaaagcgt cgacggcagc ccgccttcgc gcgccagcga agcgatgagg ccgcccttgg
      961 cggtttccgc aagcaagcgc aacgcgcgat agaggctcga tttgccgctg ccgtttggac
     1021 ccgtcacaat gttgagccgc ccgagcggga cgacgagatc gcgcaaggag cgataattag
     1081 cgacggcgag cgtggtcagc atcgataaag caggccaaat tatccatcgt cattgcgatg
     1141 agcgaagcga caaaagcaat ccagggcaat cgctcaggcg gccgctctgg attgcttcgc
     1201 ttcgttcgca atgacggcgt ggttctacat gaatgcgttc cctagcccat atagcgccca
     1261 gcgcgccccg gcttggcgat cacgtccgaa aaccgcgagt gagcgcccgc tcctgcgcta
     1321 taagtcggcg catggacctc gaccccgccc aatgctaccg cgcgctgctc gcccgcgacg
     1381 cccgctatga cgggctgttc ttcacctgcg tgaaaacgac cggcatctat tgccggccga
     1441 tctgcccggc gcggccgccg aaattcgaga attgccgctt cgtcgccacc gccgccgcgg
     1501 cgcaggaagc cggcttcagg ccctgcttgc gctgccggcc ggaaagctcg cccgatctcg
     1561 gcgcgtggcg cggcgcgtcg gcgacagtct cgcgggcgct ggctttgatc gaggacggcg
     1621 cgctcgatgc gggcgacgtc acggccctcg ccgagcggct cggcgtcggc gagcggcatt
     1681 tgcgccgcct gttcagccgt catctcggcg cggcgccggt cacggtcgcg cagacccgcc
     1741 gcgtgctgct cgccaagcag ctcattcacg agacggacct gccgatgacc gaagtcgcgc
     1801 tggcgagcgg cttcaacagc cttcgccgct tcaacgagac cttccaacag ctctatcgcc
     1861 gcccgcccag cgcgttgcgg cgccgcgccg gcgccgagac ggaagaatcc ggcctctcgc
     1921 tcctgttgcc gtaccgcccg ccctacgatt gggattcgat gatccgcttt ctcgcggcgc
     1981 gcgctttgcc gggcctcgaa atcgtcgcgg atcaacgcta cgcccgcgtc gttgaaatgc
     2041 gcggcgccgt cggctcgatc gagatcgcgc aggcgccgga ccgctcagcg ttgcgggcga
     2101 tggtgcgctt tccccgcttc gactgcctgc cgacgatcgt cgcgcgtatc cgacgcttgt
     2161 tcgatctcgg cgccgagccg atcgcgatcg gcgcggccct cgcgcgcgat ccgacgcttc
     2221 gcccgcttgt cgccgcccgg cccggactgc gcgtgcccgg cgcgtgggac gggttcgaaa
     2281 tcgccgtgcg cgccgttctc ggccagcaga tcacggtgaa agccgcgacc gggctcgccg
     2341 ccaagcttgt cgcggtcttg ggcgatccga tcggcggggg cgccggcttg cccggcttga
     2401 cgcatagctt tccgcggccg gagcggctcg acgccaattc gttggccgcg ctcggcatgc
     2461 cgaagggccg cgccgccgcc atcgccgcgc tcgccgcggc ggcgcgcgcc gatccgcatc
     2521 tcttcgatcc gcgccgcgac ctgaacgagg ccgtggcgcg cttgcgccaa ttgcccggaa
     2581 tcggcgaatg gaccgcgcaa tacatcgcga tgcgcgcgct tcgcgaaagc gacgcctttc
     2641 tcgtcgacga tgtcgcggtc cagcgcgcgc tcgccgtcga tgggcgacgg ccgcgcggcc
     2701 gcgaattgct ggcccgcgcc gagatctggc ggccgtggcg cgcgtatgcc gtgttgc
//
LOCUS       DATGJW010000307         5691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_267480, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000307 DATGJW010000000
VERSION     DATGJW010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5691
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_267480"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            508..1728
                     /locus_tag="VKU03_09335"
     CDS             508..1728
                     /locus_tag="VKU03_09335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLJ71508.1"
                     /translation="MADSLVEGSKRSEAEPDWLGLLNPQTRALEESGIVEVFNYGRNR
                     QGLIPLWVGEGDLVTPDFISEAAERSLAAGETFYTDQRGIPELRAAIARYMTRVYGQA
                     PGGGAFSPEQFFVTVGGMHALEISARLVAGPGDEIIVPSPAWPNFYGAIALAGAKTVF
                     APMIAGAEWRLDIDRIGDLVTPATRAIILNSPANPTSFTATVEELSALRDLARRRGLW
                     IIADEIYGRLVYDGARAPSFHDVMDPDDRILFVQTLSKNWAMTGWRVGWLEAPPALGP
                     VIENLVQYSTSGVPVFVQRGAEAALDGGEDFIARQIAGMRQCRDILCDGLAATGRVRF
                     AYPRAAFYLFCAFDGITDTRRLALRLVDEAGLGVAPGTAFGPGGEEYVRICFARRPEE
                     IEEATRRLARWLAK"
     gene            complement(1760..2134)
                     /locus_tag="VKU03_09340"
     CDS             complement(1760..2134)
                     /locus_tag="VKU03_09340"
                     /inference="COORDINATES: protein motif:HMM:NF016876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008657 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) inhibitor activity
                     [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_process: GO:0030541 - plasmid partitioning [Evidence
                     IEA];
                     GO_process: GO:0051290 - protein heterotetramerization
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLJ71509.1"
                     /translation="MRRRAARRAREEAISRPLVLRLTDKAEADLAEIWAYVALDSSEG
                     AATRLIDAIMKASERLRHFPLAAPARDAFAPGLRVGFAGSYAIYYVHDEQALTIVRVL
                     HSARDAAALAAQGGFAEETKAP"
     gene            complement(2068..2355)
                     /locus_tag="VKU03_09345"
     CDS             complement(2068..2355)
                     /locus_tag="VKU03_09345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribbon-helix-helix protein, CopG family"
                     /protein_id="HLJ71510.1"
                     /translation="MSEIERLTITLTAEMAAAVKHAVEIGDYASTSEVVRSALRDWKT
                     KRALQLEELAALKADIDAGLADVAAGRVKKFDAARLVARGRKLLADRSSSA"
     gene            complement(2508..3749)
                     /gene="hemA"
                     /locus_tag="VKU03_09350"
     CDS             complement(2508..3749)
                     /gene="hemA"
                     /locus_tag="VKU03_09350"
                     /EC_number="2.3.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003870 - 5-aminolevulinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006783 - heme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-aminolevulinate synthase"
                     /protein_id="HLJ71511.1"
                     /translation="MDYSRYFVEATDRLREERRYRVFAPLERSAARFPRALWRRTENG
                     VTEKREIVVWCPNDYLGMGRHPQVVAAMQEAVGRYGAGAGGTRNISGNSGPVVDLEAE
                     LADLHRKEAALVFTSGWISNLAAISTIASLLPDCLILSDALNHNSMIEGVKRSNCEKQ
                     IFRHSDLAHLERLLKARRPGRPTLIVFESLYSMDGDIAPIRAIVDLAKKYDALTYLDE
                     VHAVGLYGPRGAGVAERDGAMGEIDIVEGTLAKGFGALGGYIAASRAIIDAVRSHAPA
                     FIFTTALPPPVAAAALEAVRIAKTAGALRARHQQQVAKTKAALQQAGIPVLANPSHIV
                     PVMIGDAELCKKASDRLLERHGVYIQPINYPTVARGTERLRITPTPLHDNALIDELVA
                     ALTETWRALALPFARPAMAAE"
     gene            3953..4846
                     /locus_tag="VKU03_09355"
     CDS             3953..4846
                     /locus_tag="VKU03_09355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009467299.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HLJ71512.1"
                     /translation="MTAARPRRSVLYVPGSNPRALTKARSLAADALILDLEDSVAPEQ
                     KASAREQVAAAVREGGFGRREVVVRINALQSPWGREDLAFAAAALPDAILLPKVDGPG
                     AVMQAARELREAGAPEGVRLWAMMETPLAILNAGSIAAVSADPAARLDALVMGLNDLA
                     RETRARLTPGRSAATAWLALCVAAARAHRVDIIDGVYNDFADLAGFQRECEQGRDMGF
                     DGKTLIHPGQIAACNEIFAPTKAEIAAASAVIAAFDEPLNRDKGVIQIDGKMVERLHA
                     DMARRTLAIAEGVAALTQSAL"
     gene            4939..>5691
                     /locus_tag="VKU03_09360"
     CDS             4939..>5691
                     /locus_tag="VKU03_09360"
                     /EC_number="4.2.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fumarate hydratase"
                     /protein_id="HLJ71513.1"
                     /translation="MTYAYRPVLAIGADETPYRKLTGEGVRVEKALGREVVVVDREAI
                     RLLAEQALIDINHLLRPGHLAQLAKILDDPEATSNDKFVAYDLLKNANIAAGGVLPMC
                     QDTGTAIVVAKKGRLIWTEGDDEAAIAEGIGGAYEKKNLRYSQLAPLSMFEEKNTGDN
                     LPAQIEILAEGEDTYKFLFIAKGGGSANKSFLYQGLPSMLTHERMLAFLKEKILTLGT
                     AACPPYHLAVVIGGTSAEQTMKTVKLASTRYLD"
BASE COUNT          931 a         1903 c         1911 g          946 t
ORIGIN      
        1 cgacgccggg ccgatgcggc tttttgcatc caagagcttt gccgacgaaa ccgtcggaag
       61 cccgacggtt cgtcggcaaa gctcttggaa gccaaatcgc ctgagccgcg cggagccgtc
      121 aaggctcgcg agccatcgcg cggcgcaact gactttctcc tgagcctgct ttggcgagcg
      181 acgcgcgaag cgcgcttggc cttgacggtg agcacggctc aggcgcgccg cgcttcaaga
      241 tgcgtgcatg ccgtagcgct tcgcggggcg caggcggccc gcgtgcgcag cgccgctgga
      301 cgaggggcta tacgggactc tgaaggtacg cctccgaaag tcgctcgtca gcggacccga
      361 cgcggccgct tcgcggccct ttttccgccg cttcgcgggg cgaggggcgt cggcgccgat
      421 cacagcgcat tgccgccaat gacgcggcgc atttgggcgg cgtgagcgaa ggcgctacga
      481 tgagcgcctc aaaatttcga ggcgccgatg gccgattcgc tggttgaggg ttcaaagcgg
      541 tccgaggccg agccggattg gctcggcctc ctcaatccgc aaacgcgggc gctcgaggaa
      601 agcgggatcg tcgaagtctt caattacggt cgcaatcggc agggactcat cccgctttgg
      661 gtcggcgagg gcgatctcgt cacgccggat ttcatctcgg aggcggccga gcgctcgctc
      721 gcggccggcg agacgtttta caccgatcag cgcggaattc cggaattgcg ggcggcgatc
      781 gcccgctaca tgacccgcgt ctatggccag gcgcccggcg gcggcgcctt ctcgcccgag
      841 caattcttcg tcaccgtcgg cggcatgcac gcgctcgaaa tctcggcgcg cctcgtcgcc
      901 gggccgggcg acgagatcat cgtcccctcg ccggcttggc cgaacttcta cggcgcgatc
      961 gccctcgccg gcgccaaaac cgtcttcgcg ccgatgatcg ccggcgccga atggcggctc
     1021 gacatcgacc gcatcggcga tctcgtcacg ccggcgacgc gcgcgatcat cctcaactcg
     1081 ccggccaatc cgaccagctt caccgcgacc gtcgaggagc tgagcgcgtt gcgcgatctc
     1141 gcccggcggc gcggcctctg gatcatcgcc gacgaaatct atgggcggct cgtttacgac
     1201 ggcgcccggg cgccgtcctt ccatgacgtg atggacccgg acgaccgcat tctcttcgtc
     1261 cagaccctgt cgaaaaattg ggcgatgacc ggctggcgcg tcggctggct cgaagcgccg
     1321 ccggcgctcg gcccggtgat cgaaaatctc gttcaatatt cgacgtcggg cgtgccggtc
     1381 ttcgttcagc gcggcgcgga ggcggcgctc gatggcggcg aggatttcat cgcccggcaa
     1441 atcgccggga tgcggcagtg ccgcgacatt ctgtgcgacg gcttggctgc gaccggccgc
     1501 gtccgcttcg cctatccgcg cgccgccttc tatctgtttt gcgccttcga cgggatcacc
     1561 gatacccgcc ggctcgcgct tcggctcgtc gacgaggccg gactcggcgt cgcgcccggc
     1621 acggctttcg gcccgggcgg cgaagagtac gtccgcatct gcttcgcccg ccggcccgag
     1681 gagatcgaag aggcgacgcg gcggctggcg cgctggctgg cgaagtagga gacgcgctcg
     1741 cgccgctacg cgcgagcgtt cagggcgctt tcgtttcctc tgcgaagccg ccttgagcgg
     1801 ccagagcggc ggcgtcgcgg gcgctgtgca acacgcgaac gatcgtcaac gcttgctcgt
     1861 catgcacgta gtaaatggcg tagctgccgg cgaatcccac gcgcagaccc ggcgcaaagg
     1921 cgtcgcgcgc cggagcggcg agcgggaaat ggcgcagtcg ctcgctcgcc ttcatgatcg
     1981 cgtcgataag ccgcgtcgcc gcgccctccg acgagtccag cgcgacataa gcccagattt
     2041 ccgcaaggtc ggcttcagct ttgtccgtca ggcggaggac gagcggtcgg ctaatagctt
     2101 cctccctcgc gcgacgagcc gcgcggcgtc gaatttcttg acccggccgg ccgccacatc
     2161 cgcaaggccg gcgtcgatat cggccttgag cgccgccagc tcttcgagct ggagcgcgcg
     2221 cttggtcttc cagtcgcgca gcgccgagcg gacgacttca ctggtggagg cgtaatcgcc
     2281 gatttcgacg gcgtgtttga ccgccgccgc catttcggcg gtcagcgtga tcgtcaggcg
     2341 ctcgatctcg gacatggaaa tctccccgca gggtttcata ctttatcata ctgggagggg
     2401 ggcgccaggc ctcgacgagg cgccggaaga aacgaggcga cgcgggccta ctcatttggc
     2461 tgggaaagct gtagccgcgg ccgccgacca cggctacagc gcggcggcta ttccgcggcc
     2521 atggccggcc tggcgaaggg cagggcgagg gcgcgccatg tctcggtcag cgccgcgacc
     2581 agttcgtcga tcagcgcatt gtcatgaagc ggcgtcggcg tgatgcgcag gcgctccgtg
     2641 ccgcgcgcca cggtcggata gttgatcggc tggatataga cgccgtgacg ctcgagcagg
     2701 cggtcgctcg ctttcttgca cagttcggcg tcgccgatca tcaccggcac gatatgcgag
     2761 ggattggcga ggaccggaat gccggcctgc tgcagcgcgg ccttggtctt cgccacttgc
     2821 tgctgatggc gggcgcgcag ggcgccggcc gtcttggcga tgcgcacggc ttcgagagcc
     2881 gcggcggcga cgggcggcgg cagggcggtg gtgaaaatga aggccggcgc gtgcgagcgc
     2941 accgcgtcga tgatcgcccg gctggcggcg atatagccgc ccagcgcgcc gaagcctttg
     3001 gcgagcgtgc cttcgacaat gtcaatctcg cccatcgcgc cgtcgcgctc ggcgacgccg
     3061 gcgccgcgcg gcccgtaaag gccgaccgcg tggacctcgt cgagataggt cagcgcgtcg
     3121 tattttttgg cgagatcgac aatcgcccgg atcggcgcga tgtcgccgtc catcgaatac
     3181 aggctctcga acacgatcag ggtcggccgg ccgggccgcc gcgctttcaa caggcgctcc
     3241 aggtgagcca aatcgctgtg gcggaaaatc tgcttctcgc aattcgagcg cttgacgccc
     3301 tcgatcatcg aattgtggtt cagcgcgtcc gacaggatca ggcaatcggg caggagcgaa
     3361 gcgatggtcg agatggcggc aagattggaa atccagcccg aggtgaagac cagggccgct
     3421 tctttgcgat gcaggtcggc gagttcggct tccagatcga cgaccgggcc gctattgccg
     3481 gaaatgttgc gggtgccgcc ggcgccggcg ccgtagcgcc ccaccgcttc ttgcattgcg
     3541 gcgacgacct gcggatggcg gcccatgccg agatagtcgt tcgggcacca gacgacgatt
     3601 tcgcgctttt ccgtcacgcc gttttcagtg cgccgccaca aggcgcgggg aaaacgcgcc
     3661 gcgctgcgct cgagaggggc gaagacccgg tagcggcgct cctcgcgcag ccgatcggtc
     3721 gcctcgacga aataacgcga atagtccatg cccagctccg cggccgcaat atccctgccg
     3781 tttcgatccg ttcctataag gccgctggcc caccttatag gcgctctaaa ccggcaaagc
     3841 gaccgcagag atgtcgcaag ggggcgcccg ccgctcttcc cgccgggcct ttgccggaag
     3901 ccggatttcg ctatattgcg ccgcacaatc cgtccttctt cccggaggct tcatgaccgc
     3961 cgcccgtcca cgccgcagcg ttctctacgt gcccggctcg aacccgcgcg cgctgacgaa
     4021 agcgcgaagc ttggccgccg acgcgctgat cctcgatctc gaagattcgg tcgcgccgga
     4081 gcaaaaggcg agcgcccgcg agcaggttgc ggcggcggtg cgggaaggcg ggttcggccg
     4141 ccgggaagtg gtcgtgcgca tcaacgcgtt gcaatcgcct tggggccgcg aggacctcgc
     4201 cttcgcggcg gccgcgctgc cggacgcgat tttgctgccc aaggtcgacg ggccgggcgc
     4261 ggtcatgcag gcggcccgcg aattgcgcga ggccggcgcg ccggagggcg ttcgcctttg
     4321 ggcgatgatg gagacgccgc tcgccatcct caatgccggc tcgatcgccg cggtctcggc
     4381 ggatcccgcc gcccggctcg atgcgctcgt catggggctc aacgatctcg ccagggagac
     4441 ccgggcccgt ttgacgccgg gccgaagcgc cgcaacggcc tggctggcgc tctgcgtcgc
     4501 cgcggcgcgg gcgcatcgcg tcgacatcat cgatggcgtc tataacgact tcgccgatct
     4561 tgcgggcttc cagcgcgaat gcgagcaggg ccgcgatatg ggattcgacg gcaagacgct
     4621 catccatcca ggccagatcg ccgcctgcaa cgaaatcttc gcgccgacca aggcggaaat
     4681 cgcagcggcg agcgcggtga tcgccgcttt tgacgagccg ctcaatcgcg acaagggcgt
     4741 catccagatc gacggcaaaa tggtcgagcg cctgcacgcc gacatggcgc gccggacgct
     4801 ggcgatcgcc gagggcgtcg ccgcgctaac gcagagcgcg ctgtagccgc ggtcgccgac
     4861 cgcggctaca acctccactt ccgcgcaatg gccgcggtcg ccgaccccgg ttacaaaccc
     4921 ctgagaaggc gcatacctat gacctatgct tatcgcccgg tccttgcgat cggcgccgat
     4981 gaaacgccct atcggaaatt gaccggcgag ggcgtccgcg tcgagaaggc gttgggacgc
     5041 gaagtcgtcg tcgtcgaccg cgaggcgatt cgcctgttgg ccgagcaggc gttgatcgac
     5101 atcaaccatc tgttgcggcc gggccatctc gcccagctcg ccaaaatcct cgatgacccc
     5161 gaggcgactt cgaacgataa attcgtcgcc tacgatctcc tgaagaacgc caatatcgcc
     5221 gcgggcggcg tgctgccgat gtgccaggac accggcacgg cgattgtcgt cgccaaaaaa
     5281 ggccggctga tctggaccga gggcgacgac gaagccgcga tcgccgaagg cattggcggc
     5341 gcgtacgaaa agaagaacct tcgctattcg cagctcgcgc cgctctcgat gtttgaggag
     5401 aagaataccg gcgacaacct gccggcgcag atcgaaatcc tcgccgaggg cgaggatacg
     5461 tacaagtttc tgttcatcgc caagggcggc ggctcggcca acaagagctt tttgtaccag
     5521 ggcttgccgt cgatgctgac gcatgagcgg atgctcgcct ttctcaagga gaaaatcctc
     5581 acactcggca ccgccgcttg cccgccctac catctcgccg tggtgatcgg cggaaccagc
     5641 gccgagcaga cgatgaaaac cgtcaagctc gcctcgaccc gctatctcga c
//
LOCUS       DATGJW010000308         6952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_888780, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000308 DATGJW010000000
VERSION     DATGJW010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6952
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_888780"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(47..1495)
                     /gene="dnaA"
                     /locus_tag="VKU03_09365"
     CDS             complement(47..1495)
                     /gene="dnaA"
                     /locus_tag="VKU03_09365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003688 - DNA replication origin binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006270 - DNA replication initiation
                     [Evidence IEA];
                     GO_process: GO:0006275 - regulation of DNA replication
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromosomal replication initiator protein DnaA"
                     /protein_id="HLJ71514.1"
                     /translation="MLQPNDDFPNRLEAWKRCTMRLRAELGEDVYSSWFGRLELDEIV
                     DGEARLSVPTKFLKSWIDNHYTDRILAAMKSEFADVVALTLSVRSSTRPALSVLDARP
                     KLGAPESGPIAPVAEREPPRPSGKRDAPASEEAMAGSPLDRRLTFATFLVGKSNQLAY
                     SAAQKIIETRQGQPPAFSPLYIHAAVGLGKTHLLQAVAHAATSQGRRAIYLTAERFMY
                     GFVAALRAQTAIAFKEALRSIDLLIFDDAQFLQGRSIQNEFCHALNALVDAGRQVVVA
                     ADRPPNELESLEERVRSRLAGGLCVEMGALDEALRVKILEARVAAAQALQPGFDVAPP
                     VLAYVAKTINSNGRDLEGAVNRLLAHSSIAGQRHTVESAEVAIRDLVRTREPKRVKIE
                     DIQKLVANRFSVTRADILSARRTATVVKPRQIAMYLAKVLTPRSLPEIGRRFGGRDHT
                     TVLHAVRKIEGLAKTDANLNEELELLKRMLLE"
     gene            complement(1573..2019)
                     /locus_tag="VKU03_09370"
     CDS             complement(1573..2019)
                     /locus_tag="VKU03_09370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HLJ71515.1"
                     /translation="MSEQSSIGYAGDLSASDAYSLLERDEKAVLVDVRTKAEWAYVGV
                     PDLSALGKQALLVEWQEFPSMARNPSFAATVEKALRDSGAGPDAPVLFLCRSGARSRA
                     AAIAMTQAGHTQCFNIAEGFEGPLDGGRHRGAVDGWKAQGLPWSQS"
     gene            2582..3355
                     /gene="tam"
                     /locus_tag="VKU03_09375"
     CDS             2582..3355
                     /gene="tam"
                     /locus_tag="VKU03_09375"
                     /EC_number="2.1.1.144"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910294.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030798 - trans-aconitate
                     2-methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trans-aconitate 2-methyltransferase"
                     /protein_id="HLJ71516.1"
                     /translation="MSDWDSRQYLQFEAERTRPAADLLAQVKGSARRVVDLGCGPGNS
                     TEILAARFPDAEALGLDNSDDMLAKARARLPKIKFDKADISTWRPASAPDVIFANAVM
                     QWTPRHIELMARLLDCLAPSGRLAVQMPDNLAEPTHALMRETAALAPFAVKLAHAAAS
                     RERIGTFEEYYAALSPRAARLDMWRTTYAHILEGAPAIVEWVKGTGLRPFLDPLTPEE
                     KRDFLARYGAAIAEAYPSLPDGRALLRFPRLFIVAAKSA"
     gene            3359..4318
                     /gene="mepA"
                     /locus_tag="VKU03_09380"
     CDS             3359..4318
                     /gene="mepA"
                     /locus_tag="VKU03_09380"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661546.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-insensitive murein endopeptidase"
                     /protein_id="HLJ71517.1"
                     /translation="MAARLRSTLKALLAAGAVRGAPLLVAVGLACALSGLAQGQDKGT
                     LNPRPLPPLANPNDPDLPAKELFGRATTPTEGEARSIGFYSRGCLAGAKALPADGPTW
                     QVMRLSRDRYWGNPVLLAFIERFSGKAAAAGVWPGILIGDMAQPRGGPMITGHASHQI
                     GLDADIWLTPMPDRRLTREEREEMSAVNMVREDGLAVDPHWSERQAGIIKAAAEEPEV
                     ERIFVNAAIKKALCRTHKGEAWMTKVRPYWGHTYHFHVRLACPKGETECRRQEPVPPG
                     DGCDKSLDWWFTDDALHPKPGPPRKPLTLAQLPPACRQVLLEK"
     gene            complement(4382..4894)
                     /locus_tag="VKU03_09385"
     CDS             complement(4382..4894)
                     /locus_tag="VKU03_09385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00645 family protein"
                     /protein_id="HLJ71518.1"
                     /translation="MIERVIERILFGSRWLLAPFYLALAIGLIELLIKTAEKLYDLTK
                     AFLGMTESGMILGVLSIVDLTLTASLVLIVIFSGYVNFVSRIDASEHADWPHWMASID
                     FSELKLKVMASIVAISAIKLLEAYMDIDQTSDRDLWFLAGIHLTFVVSTLILALADRF
                     GDHQESETPH"
     gene            complement(4935..5378)
                     /locus_tag="VKU03_09390"
     CDS             complement(4935..5378)
                     /locus_tag="VKU03_09390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019998144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HLJ71519.1"
                     /translation="MRILLCFAVGLLFGVGLCVSGMTHPDKVLGFLDLAGAWDPSLAF
                     VMIGAVLVSFGAFRLAKQRPRDALGAPFALPPRGAIDRRLIIGSVIFGVGWGLAGICP
                     GPGIVDIGFLEPRALVFVAAVAVGMVIHQLAKLRGPAQKAIEQDA"
     gene            complement(5388..5819)
                     /locus_tag="VKU03_09395"
     CDS             complement(5388..5819)
                     /locus_tag="VKU03_09395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE family protein"
                     /protein_id="HLJ71520.1"
                     /translation="MTIDWAHFTPGSAFVGGLLIGLAASALILGAGRIMGAAGILGGA
                     LAAKPGDTAWRLALIAGLLLAPGLLALFGAADRPHFFVSWPLLIVSGLLVGFGTRLAS
                     GCTSGHGVCGVARLSPRSLVATSLFMLAGFATVFCVRHLLA"
     gene            complement(6026..6901)
                     /locus_tag="VKU03_09400"
     CDS             complement(6026..6901)
                     /locus_tag="VKU03_09400"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HLJ71521.1"
                     /translation="MAKAIVGVIGGSGVYDLPGLTDLREEKVSSPWGEPSDSLRFGRI
                     GATEAVFLPRHGRGHRFSPSGINYRANIDALKRAGVTDIVSVSACGSFKRELYPGLFV
                     LIDQFEDRTYRRDNSFFGNGCVAHVSMAHPIAPLLQRRIADAAAAENIPFQMNGTYVC
                     MEGPQFSSLAESLRYQAQGADVIGMTAATEAKLAREAELSYATIAMVTDYDCWHEEHG
                     AVDVAAVIKVVQANAARAAALVGRLLRDFPAEHEPCPIGSDRALDNAIMTHPALRDPA
                     LMTKLDAVAGRLLGG"
BASE COUNT         1276 a         2197 c         2332 g         1147 t
ORIGIN      
        1 acgattttga aaatgagcgc catggctcaa gcgggacgca ttcggctcat tcgagcaaca
       61 tgcgcttgag cagctccagc tcttcgttga gattggcgtc ggtcttggcg aggccttcga
      121 tcttgcgcac cgcgtgcagc acggtcgtat ggtcgcgccc gccgaagcgg cgaccgatct
      181 cgggcagaga gcgcggcgtc aggactttcg caaggtacat cgcgatctgc cgcggcttga
      241 cgaccgtggc ggtgcggcgc gccgagagga tgtcggcgcg ggtcacgctg aagcggttgg
      301 cgaccagctt ttggatgtcc tcgatcttga cgcgcttggg ctcgcgcgtg cggacgagat
      361 cgcggatcgc cacctcggcg ctttcgaccg tgtggcgctg cccggcgatc gaggaatggg
      421 cgagcaggcg gttgaccgcg ccttcgaggt cgcggccgtt ggaattgatc gtcttggcga
      481 cataagcgag gactggcggg gcgacgtcga agccgggctg caaggcctgc gcggccgcga
      541 cgcgcgcttc gaggattttc acgcgcaaag cttcgtcgag cgcgcccatc tcgacgcaga
      601 gcccgccggc gaggcgagag cgcacgcgct cctcgagcga ttccagctcg ttcggcggcc
      661 ggtcggccgc gaccacgact tgccgtccgg cgtcgaccag cgcgttcagc gcatggcaaa
      721 actcgttctg gatggagcgg ccttgcaaaa attgcgcgtc gtcgaaaatc aacaaatcga
      781 tcgagcgcag cgcctccttg aaggcgatcg cggtttgcgc gcgcaaagcg gcgacgaagc
      841 cgtacatgaa gcgctcggcc gtcaggtaaa tcgcgcgccg gccctggctg gtcgccgcat
      901 gcgccacggc ttgcagcaga tgggtcttgc cgagcccgac ggcggcgtgg atgtaaagcg
      961 gactaaaagc cggcggctgg ccttgccggg tttcgatgat tttctgcgcc gcggaatagg
     1021 cgagctggtt cgatttgccg acgaggaagg tcgcaaacgt caggcggcgg tcgagcggcg
     1081 agccggccat ggcttcttcg ctcgccggag cgtcgcgttt tcccgatggg cgcggcggct
     1141 cgcgctcggc gaccggcgcg atcgggccgg attctggcgc gccgagtttc ggccgcgcgt
     1201 cgaggacgct caaagccggg cgcgtcgacg agcggaccga aagcgtcagc gcgacgacat
     1261 cggcgaactc gctcttcatc gccgcgagaa tgcggtcggt atagtgattg tcgatccagc
     1321 tcttcaggaa tttcgtcggc accgacagac gcgcttctcc gtcgacgatc tcgtcgagct
     1381 caagccggcc gaaccagctc gaatagacgt cttcgccgag ctcggcgcgc aagcgcatcg
     1441 tgcatcgctt ccaggcttcc agacggttcg gaaagtcgtc gttcggctgc agcatggcgc
     1501 aattcttcat ctgtgttgtt gtgacggcct cggcgcgcgc cgaaatcgaa gggtggagca
     1561 tggcgaagcc ctttaagatt gagaccaggg aaggccctgc gccttccatc cgtcgaccgc
     1621 gccgcgatgg cgcccgccgt cgagcggccc ttcgaatcct tcggcaatgt tgaaacactg
     1681 cgtatggccg gcttgagtca tggcgatggc ggcggcgcgc gagcgcgcgc ctgagcggca
     1741 gaggaaaagt accggcgcgt ccggcccggc gccgctgtcg cgcagcgcct tttcgacagt
     1801 cgccgcgaaa gacggattgc gcgccatcga ggggaattcc tgccactcga cgagcagcgc
     1861 ctgcttgccg agagccgaaa ggtcggggac gccgacatag gcccactccg ctttcgtgcg
     1921 gacgtcgacg aggaccgctt tctcgtcgcg ttcaagcagc gaataggcgt ccgatgcgga
     1981 cagatcgccc gcatagccga tactggattg ctcagacatg atcttccctt tccgcagacg
     2041 ccgcgccgcg gatgatgagc cgttcgattg aagagcgtag aagcgagcta tgaaatcaat
     2101 aaggtcagaa cgagcttatt ttctaaggcc tggcggcgct tatgccgatt ttccggagca
     2161 agcacgacgt ccccctctga ccgccaatgc tggacagcca ttgaaaatat tatcgttgat
     2221 gtaagcgtcg tcgggcattg ccgtcgacgg aaaatttatc gcatggtcgc tggaggcggg
     2281 caacatgagt cgtcatcaaa ccgtcgcagt cgggccggcg cggacgccat gtgaataaca
     2341 ggccgcatct ggccgaagcg caaaacgttg tgtcgtaagg aaagtttctt ttgcaacact
     2401 gccgaaaaaa aactcccgcc gagatgaatt tggcgcttgt gcgacgaaac cttgacgatc
     2461 cagcgcctcg cgaaaccggg ctttgcccgc aactggttga atcgcagacg gtttgcatgc
     2521 gcgttttggc cgggcgcgcc ggcgcggcgt caaccccttt gtcgaaggga gatttcgccc
     2581 gatgagcgat tgggactcgc ggcaatatct acaatttgaa gcagagcgca cgcgtccggc
     2641 cgccgatctg ctggcgcaag tcaagggatc ggcgcgccgc gtcgtcgatc ttggttgcgg
     2701 gcccggcaat tcgactgaaa tcctggcggc gcgctttccc gacgccgagg cgctcggtct
     2761 cgacaattcc gacgacatgc tcgccaaagc gcgcgcgcgg ctgccgaaga tcaaattcga
     2821 caaggccgac atctctacat ggcggcccgc ttcggcgcct gacgtgattt tcgccaacgc
     2881 ggtcatgcaa tggacgccgc gccatatcga gctcatggcg cgtttgctgg attgcttggc
     2941 gccgagcggg cgattggcgg ttcagatgcc cgacaatctc gccgagccga cgcacgcctt
     3001 gatgcgcgag accgccgcgc tcgcgccttt cgccgttaag ctcgcgcatg ccgcagcttc
     3061 gcgcgagagg atcggaacgt tcgaggagta ctacgccgct ctgtcgccgc gcgcggcgcg
     3121 cctcgacatg tggcgcacga cctacgcgca tattctcgaa ggcgcgccgg cgatcgtcga
     3181 atgggtcaag ggaaccgggc tgcgcccgtt tctcgatccg ctcacgcccg aagagaaacg
     3241 ggactttctt gcgcgctacg gcgcggcgat cgccgaggcc tatccgtctt tgccggacgg
     3301 gcgcgcgctc ctgcgctttc cgcgcctctt catcgtcgcc gcgaaaagcg cttgaaccat
     3361 ggcggcgcgg ctgcgatcga cgctcaaagc gctgctggcg gccggcgccg tacgcggcgc
     3421 gcctttgctt gtcgctgtcg gcctcgcttg cgcgctgagc ggtctcgcgc agggccagga
     3481 caagggaacg ctgaacccac ggcctttgcc gccgctcgcc aatccgaacg atcccgacct
     3541 tccggccaag gaattgttcg gccgcgccac gaccccgacc gaaggcgaag cgcgctcgat
     3601 cggcttttat tcgcgcggct gccttgccgg cgccaaggct ttgccggccg acggtccgac
     3661 atggcaagtg atgcgcctgt cgcgcgaccg ctattggggc aatccggttc tgctcgcctt
     3721 tatcgagcgc ttctccggga aagccgcggc cgcgggcgtg tggccgggca ttcttatcgg
     3781 cgatatggcg cagccgcgcg gcggcccgat gatcaccggc cacgcctcgc accaaatcgg
     3841 cctcgacgcc gatatctggc tgacgccgat gcccgatcgt cgcctgacgc gcgaggagcg
     3901 cgaggagatg tcggcggtca atatggtgag ggaggatggg ctcgcggtcg atccgcattg
     3961 gtcggagcga caggcgggaa ttatcaaggc cgccgccgag gagccggagg tcgagcgcat
     4021 tttcgtcaac gccgcgatca agaaggctct ttgccggacc cataaaggcg aggcctggat
     4081 gacgaaagta cgtccgtatt gggggcacac ttaccatttc catgtccgcc tcgcctgccc
     4141 gaagggcgag acggaatgcc gccggcaaga gcccgtcccg cccggcgacg gctgcgacaa
     4201 gtcgctcgat tggtggttca ccgacgacgc gctgcatcct aagcccgggc cgccgagaaa
     4261 gccgctgacc ttggcgcagt tgccgcccgc ctgccgccag gtgctgctgg agaagtgagg
     4321 ggaaactcct tctccccgcc ttgcgcttcg cggcgagaag gggttctccc gccgccgtcc
     4381 ctcaatgcgg cgtttcgctt tcctggtggt cgccgaaccg gtcggccagc gccaggatta
     4441 aggtcgagac cacgaaggtg agatggatgc cggccaggaa ccagaggtcg cggtccgagg
     4501 tctggtcgat atccatatag gcttcgagca gcttgatcgc ggagatcgcg acgatcgagg
     4561 ccatgacttt gagcttgagc tcgctgaaat cgatactggc catccaatgc ggccaatcgg
     4621 cgtgctcgct ggcgtcgatc cgcgagacga aattcacgta gccggaaaag atcacgatca
     4681 acaccagcga cgcggtgagg gtcaggtcga cgatcgacag gacgccgaga atcatcccgg
     4741 attcggtcat gccgagaaag gctttggtga ggtcgtaaag tttttccgcc gtcttgatca
     4801 gaagctcgat caagccgatc gccagcgcga gatagaaagg cgcgagcagc caccggctgc
     4861 cgaacagaat tctctcgatg acccgttcga tcatgaagcg cagcccccgg cgcgaagaga
     4921 cctaagcttc cgcgtcaggc gtcttgttcg atcgcttttt gcgccgggcc gcggagcttg
     4981 gcgagttgat gaattaccat gccgaccgcg accgccgcca caaagaccag cgcccgcggt
     5041 tcgagaaagc cgatatcgac aatgcctggg ccggggcaga tcccggcgag gccccagccg
     5101 acgccgaaaa tcacgctgcc gatgatcagg cggcggtcga tggcgccgcg cggcggcaaa
     5161 gcgaaaggcg cgccgagcgc atcgcgcggc ctttgcttgg cgagcctgaa cgcgccgaag
     5221 ctgacgagaa ccgcgccgat catgacgaaa gccagggacg ggtcccacgc gccggcgaga
     5281 tcgaggaagc ccagcacctt gtccggatgg gtcatgcccg agacgcaaag gccgacgccg
     5341 aagagcaggc cgacagcaaa gcagagcaga atgcgcatcg ttcgtcctca cgcgagcagg
     5401 tggcggacgc agaagaccgt cgcgaagccg gccagcatga agaggctggt cgcgacaagc
     5461 gagcgcggcg aaagccgcgc cacgccgcaa acgccgtggc cgctggtgca gccggaggcg
     5521 agccgcgtgc cgaagccgac cagcaggccg ctgacgatca gcagcggcca ggaaacgaaa
     5581 aaatgcgggc ggtcggccgc gccgaataga gcgagcaggc cgggcgcgag caacaggccg
     5641 gcgatcaacg cgagacgcca ggcggtatcg ccgggcttgg cggcgagcgc gccgccgaga
     5701 attcccgccg cgcccatgat ccgtccggcg ccaaggatca aggccgaggc ggcaaggccg
     5761 atcagcagcc cgccgacaaa ggccgatccc ggcgtgaaat gagcccagtc gatcgtcatc
     5821 gacgcctcca gaggccgcaa acgtcgcgcg gccgtggagc taagctatga gttgcggcgc
     5881 ctaatgcaag cgggttcaga cattgcacgg ctccaacgcc caaacgccat cattgcgagg
     5941 acccgagcag caatccatac ggcgttcgcg atcgctgcag ccgccagatg gattgcttcg
     6001 ccgcgcgcgc aatgacggca tcgcttcagc cgccgaggag gcggccggcg acggcgtcga
     6061 gcttcgtcat cagagccggg tcgcgcagag ccggatgcgt catgatcgcg ttgtcgaggg
     6121 cgcggtccga gccgatcggg cagggctcgt gctcagcggg aaaatcgcgc aagagccggc
     6181 cgaccaaggc ggcggcgcgg gcggcgttgg cttgcacgac tttgatcacc gcggcgacgt
     6241 cgaccgcgcc atgctcctcg tgccagcaat cgtaatcggt gaccatggcg atggttgcat
     6301 aggaaagctc ggcctcgcga gcgagcttgg cctcggtcgc cgcggtcatg ccgatcacgt
     6361 ccgcgccttg cgcctggtag cgcagcgact cggcgaggct cgagaattgc ggcccctcca
     6421 tgcagacgta agttccgttc atctggaacg gaatattctc cgccgccgcg gcgtcggcga
     6481 tccgccgctg aagcagcggc gcgatcggat gggccatcga gacatgggcg acgcagccgt
     6541 tgccgaagaa gctgttgtcg cggcggtagg tccggtcctc gaactggtcg atcagcacga
     6601 acaggcccgg atagagctcg cgcttgaacg agccgcaagc cgagaccgag acgatgtcgg
     6661 tgacgccggc gcgcttcagc gcgtcgatat tggcgcggta attgatcccg gacggagaaa
     6721 agcggtggcc gcggccatgg cgcggcagga acaccgcctc cgtcgcgccg atccggccga
     6781 agcgcaggga atcggacggc tcgccccaag gcgaggagac tttttcttcg cgcaggtccg
     6841 tcaagccggg aaggtcatag acgcccgagc cgccgataac gccgacgatc gctttggcca
     6901 tgatgcgccc cttgtacccc tcgccccgcg cagcggggag agggtggccg cg
//
LOCUS       DATGJW010000309         4666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_59097, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000309 DATGJW010000000
VERSION     DATGJW010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59097"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..758)
                     /locus_tag="VKU03_09405"
     CDS             complement(<1..758)
                     /locus_tag="VKU03_09405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252060.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ71522.1"
                     /translation="MKIAYFTHSLASCWNHGNAHFLRGVLRELLFSGHDVAAFEPLGA
                     WSLAQLLRDHGEAGLAPYREAYPELSSTAYSRDFDFEAALDGCGLVVVHEWSEPWLVA
                     AIGALRRRGARFTLLFHDTHHRAVSDPQAIRAFDLNGYDGVLAFGEALATVYRRWGWG
                     DRVFVWHEAADTRLFRPPSEEQPRRGLVWIGNWGDEERSAELEEFLLAPAQSANLPLD
                     VFGVRYPAEAQAMLQRHGARLRGWLANALVPSVFA"
     gene            complement(755..1891)
                     /locus_tag="VKU03_09410"
     CDS             complement(755..1891)
                     /locus_tag="VKU03_09410"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLJ71523.1"
                     /translation="MPRPLNPPIRRLLMTTDSVGGVWTYALDLSRALARRGVSVTLAV
                     LGPMPSDAQRREAENVKGVKLVMTEAQLDWLCEEPGAARGAPALLSALARDFGVDLIQ
                     LNGAPFAAEAEYPAPVVAVHHSCLATWWASVRNSPPPPEWRWRADLVRRHLEAADRVV
                     APSAAFAALTTATYGLNRRIDIVHNGRSRSGFAPSGKTFDALFTAGRLWDEGKNVATL
                     DRAAPLVDAPFFAAGPLAGPNGIKVDCRNLQTLGELSSAAVRAWLAKRPIFLSSAAYE
                     PFGLTALEAAQAGCALVLADIPTFRELWDRAALFAPPRDHAAFGKAATALLDDRLLRR
                     RMERAAHERARRYSLEAMGARMFALYEELVGTGAPAPGDRERAA"
     gene            complement(1876..3168)
                     /locus_tag="VKU03_09415"
     CDS             complement(1876..3168)
                     /locus_tag="VKU03_09415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012778969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04295 family B12-binding domain-containing
                     radical SAM protein"
                     /protein_id="HLJ71524.1"
                     /translation="MRTALVNPSWRFDRSIYFGCREPHLPLELGASRALLQAAGHEAK
                     IFDGHLCGQSTAELAGAVAAYQPDMTVVTTAPSYLFWRCAPPELKVPREFFIALGRRG
                     GCRVAVGPHGSATPATTLRKLDADVVVRGECEEIVAALADATELASVPSIAFRDDDEI
                     RVTGAPAAASFVDLPALFWPDKWILRHQHHHHRFEAAPDGPGAEVEASRGCPYSCSFC
                     AKIDYRDKYRRRALPPLLEEIDALIAQGAAYLYFIDEIFLPQKPLLEALVSRNIKFGV
                     QTRIDLWKPDMLDLLGAAGCVSIEAGVESLTAEGRAHLDKNCRLSTDELADRLIYARQ
                     RVPFVQANLIKMPDDDRDLIDAWRERLSAHRVWANDPVPLYPYPSSPDYRRLWGAPDE
                     KAWERAHAYYLDQFESFSDIQDERPLPLSDLEAQCLAR"
     gene            complement(3165..4250)
                     /locus_tag="VKU03_09420"
     CDS             complement(3165..4250)
                     /locus_tag="VKU03_09420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71525.1"
                     /translation="MNAPGIVGALHPVVVTGGAGFIGSNLADRLAREGRHVLLFDSLR
                     RAGAERNARWLRERHPERISLQRCDIRDQTAVRAIVSEASAVFHFAAQVAVTTSLLDP
                     TEDFAINVVGGFGLLEAARRQNRALPFIFASTNKVYGDLADIPLVEEKDCYAPLDAAI
                     RANGVSEERPLDFHTPYGCSKGAADQYVLDYARSFDLPAAVMRMSCIYGPRQMGTEDQ
                     GWVAHFLIRALRGEAITIFGDGKQVRDILYVDDAVNAYLAAWRNIERVKGRAFNLGGG
                     PANAVSLRTLIAFIEGRLGRPVETRYEAWRPGDQRYYVSNAQAAEHELGLRQPLPWRE
                     GVARLIDWLRREYPSSPASARQKEAAE"
     gene            complement(4247..>4666)
                     /locus_tag="VKU03_09425"
     CDS             complement(4247..>4666)
                     /locus_tag="VKU03_09425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605349.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HLJ71526.1"
                     /translation="RLANNKPPLVFEDGRQRRDFVHVDDVAEAFAVALEHPKAPGEIY
                     NIGSGQDRSILQIAEMLAAAMNKADIAPEVSGQAREGDIRHCFADTGKAAKELGFVAR
                     QDFSQGLTALAEWVASQHAHDRLAEARSELERRGLVA"
BASE COUNT          847 a         1497 c         1571 g          751 t
ORIGIN      
        1 gcgaaaacgg atggtacgag cgcattggcc agccagccgc gaagccgggc gccgtggcgc
       61 tggagcatgg cctgcgcctc cgcgggatag cgcacgccaa agacatcgag cggcaggttc
      121 gcgctttgcg cgggcgcgag caggaattcc tcaagctccg ccgaacgctc ctcgtcgccc
      181 caattgccga tccagacgag gccgcggcgg ggttgctctt cgcttggcgg gcggaaaagg
      241 cgcgtgtcgg ccgcctcatg ccagacgaag acgcggtcgc cccagcccca ccgccgatag
      301 accgtggcca gcgcctcgcc gaaggcgagc acgccgtcat agccgttcag atcgaaggcg
      361 cggatcgcct gcgggtcgct aaccgcgcga tgatgcgtat cgtgaaagag cagcgtgaac
      421 ctcgcgccgc gccggcgcag cgcgccgatc gcggcgacaa gccagggctc gctccattcg
      481 tggacgacga cgagcccgca accgtccagc gcggcttcaa aatcgaaatc gcgggaatac
      541 gcggtcgagg agagctcggg ataggcttcg cgatagggcg cgagcccggc ttcgccgtga
      601 tcgcgcagca attgcgcaag gctccacgcg cccagaggtt cgaaggcggc gacgtcatgg
      661 ccggaaaaaa gaagctcgcg caacacgccg cgcaggaaat gcgcgttgcc gtgattccag
      721 cacgaggcga gagaatgcgt gaaataggcg attttcacgc ggcgcgctcc cgatcgcccg
      781 gcgccggcgc tcctgttccg acgagctctt cgtaaagagc gaacatgcgc gcgcccatcg
      841 cttcgaggct gtagcggcgc gcgcgctcat gcgccgcgcg ctccatgcgc cgacgcagga
      901 gccgatcgtc gaggagcgcc gtggcggctt tgccgaaggc ggcgtggtcg cgcggcggcg
      961 cgaaaagcgc cgcgcggtcc cacaattcgc ggaatgtcgg aatatccgcg aggactagcg
     1021 cgcaaccggc ctgggccgcc tccaacgcgg tcaggccgaa cggctcgtaa gccgcgcttg
     1081 agaggaagat tggcctcttg gccagccagg cgcgcacagc ggcgctgctg agttcgccaa
     1141 gagtttggag gttgcggcaa tcgaccttga tcccattcgg tccggcaagc ggaccggccg
     1201 caaaaaacgg cgcgtcgacc aagggcgcgg cgcggtcgag cgtcgcgaca ttcttgccct
     1261 cgtcccacaa acggccggcg gtgaacagcg catcgaaggt cttgccggac ggcgcgaagc
     1321 cgctccgcga acggccgtta tggacgatgt cgatccgcct gttcagacca taggtcgctg
     1381 tcgtgagcgc ggcgaaagcg gcgctcggcg cgacgacccg atccgccgcc tcgagatgac
     1441 gacggacgag atcggcgcgc catcgccatt ctggcggcgg cgggctattt cttacgcttg
     1501 cccaccatgt ggcgaggcac gagtgatgga ctgcgacgac gggcgcggga tactcggcct
     1561 cggcggcgaa gggggccccg ttcaactgga tgaggtcaac gccgaaatcc ctcgctaggg
     1621 ctgagagcag cgccggcgcg ccgcgcgccg cgccgggctc ctcgcataac cagtcgagct
     1681 gcgcttcggt catgacgagc ttgacgcctt tgacgttctc ggcttcgcgc ctttgcgcat
     1741 cggacggcat aggtccgaga accgcgagcg tgacggagac gccgcggcgc gccagcgccc
     1801 gggaaagatc gagcgcataa gtccataccc cgccgaccga gtcggtggtc atcagcagac
     1861 ggcggatcgg cggattcaac gggcgaggca ctgcgcctcc aggtccgaca ggggaagggg
     1921 ccgttcgtcc tgaatatcgc tgaagctttc gaactgatcg aggtaatagg cgtgcgcgcg
     1981 ctcccaggcc ttttcgtcgg gcgcgcccca aaggcggcgg taatcgggcg aactcggata
     2041 gggatagagc ggcaccggat cgttcgccca gacgcgatgc gccgacaagc gttcgcgcca
     2101 tgcgtcgatc aggtcgcgat cgtcgtccgg cattttgatc agattggcct gtacgaacgg
     2161 aacgcgttgg cgcgcataga tgaggcgatc ggccagttca tcggtcgaga gccggcagtt
     2221 cttgtcgaga tgcgcgcggc cctccgccgt caggctttcg acgccggcct cgatcgacac
     2281 gcagccggct gcgccgagaa ggtccagcat atcgggtttc caaagatcga tccgcgtctg
     2341 cacgccgaac ttgatgttcc gcgagacgag cgcctcgagc agcggctttt gcggcaggaa
     2401 gatctcgtcg atgaagtaga gatacgccgc gccttgcgcg atcagggcgt cgatttcctc
     2461 gagtagcggc ggcagggcgc gtcggcgata tttgtcgcga tagtcgattt ttgcgcagaa
     2521 gctgcaggaa taaggacagc cgcgcgacgc ttccacttcc gcgccgggac cgtccggcgc
     2581 cgcctcgaag cgatgatgat ggtgttgatg gcggagaatc cacttatctg gccagaacag
     2641 agccggcaga tcgacgaagc tggcggcggc gggcgcgccg gtcacgcgaa tctcatcgtc
     2701 gtcccggaac gcgatcgaag gaacgctcgc aagctccgtc gcatcggcta gggcggcgac
     2761 aatctcttcg cattcgccgc gcacgaccac gtccgcgtcc agcttgcgca aagtcgttgc
     2821 aggcgtcgcg gagccgtgtg gcccgacggc gacgcggcag ccgccgcggc ggccgagagc
     2881 gatgaaaaat tcgcgcggga ctttgagctc cggcggcgcg caacgccaga aaaggtagct
     2941 tggcgcggtc gtcaccaccg tcatgtcggg ctggtaggcg gcgaccgcgc cggcaagctc
     3001 cgccgtcgat tggccgcaga gatggccatc gaatattttc gcttcatgcc cggccgcttg
     3061 cagcaacgct cgtgacgccc cgagttcgag cggcagatgt ggttcgcggc agccgaaata
     3121 gatgctccga tcgaagcgcc acgatggatt gacgagcgct gttctcactc cgccgcctcc
     3181 ttctgtcgcg cggaggcggg agacgaaggg tattcgcggc gcagccagtc gatcagccgg
     3241 gcgacgcctt ctcgccaagg cagaggctgg cgcagaccca gttcatgctc cgccgcttgg
     3301 gcgttagaga cgtaatagcg ctgatcgccc ggccgccagg cctcatagcg ggtttcgacc
     3361 ggtcggccga gccgtccttc gatgaaggcg atcaaagtcc gcaggctcac cgcgttcgcc
     3421 gggccgccgc caagattgaa ggcgcggccc ttcacccgtt cgatattgcg ccatgcggcg
     3481 agatacgcgt taacggcgtc gtcgacgtac aaaatgtcgc gaacctgctt gccgtcgccg
     3541 aaaatcgtga tcgcttcgcc gcgaagcgca cgaatcagga aatgagcgac ccagccctga
     3601 tcctcggtgc ccatctggcg cggcccgtaa atgcagctca tgcgcatcac cgcggcgggc
     3661 aggtcgaaac tgcgcgcata gtcgagaaca tattgatcgg ccgcgccttt cgagcagcca
     3721 taaggcgtat gaaaatcgag cggccgctcc tccgagacgc cattggcccg gatcgcggcg
     3781 tcaagcggcg cataacaatc tttttcctcg accagaggaa tgtcggcgag gtcgccatag
     3841 accttgttcg tcgacgcgaa gatgaagggc agggcgcgat tttgtcgtcg agccgcttcg
     3901 agcaggccga atccgccaac gacattgatc gcgaaatctt ccgtcggatc gagcaagctg
     3961 gtcgtgacgg cgacctgggc ggcgaaatgg aagacggcgc tagcttcgct gacgattgcg
     4021 cgaaccgccg tttggtcgcg aatgtcgcag cgttgcaaag aaatgcgctc cgggtgccgc
     4081 tcgcgcagcc agcgcgcatt gcgctcggcg ccggcccggc gcagcgagtc gaaaaggagg
     4141 acatggcggc cttcccgcgc caaacggtcg gcgagattgc tgccgatgaa tccggcgccg
     4201 cccgtcacaa caacgggatg cagggcgccg acaattcctg gcgcgttcat gcgacgagcc
     4261 cccgccgctc cagttcgctt ctcgcctcgg caagacggtc atgggcgtgc tggctcgcga
     4321 cccattcggc cagcgcggtc aggccttggc tgaaatcctg acgggcgaca aagccgagct
     4381 ccttcgcggc cttgcccgta tcggcgaagc aatggcgaat gtcgccttcg cgcgcttgac
     4441 cgctgacctc cggcgcgatg tcggctttgt tcatcgccgc agccagcatc tcggcgattt
     4501 gcaggatgct gcggtcctgc ccgctgccga tgttgtaaat ctcgccgggg gctttcggat
     4561 gctccagcgc cacagcgaag gcttcggcga catcgtcgac atgcacaaaa tcgcggcgct
     4621 ggcgtccatc ctcgaagacg agcggcggct tgttgttcgc gaggcg
//
LOCUS       DATGJW010000310         2058 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_681059, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000310 DATGJW010000000
VERSION     DATGJW010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2058)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2058)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2058
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681059"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..383)
                     /locus_tag="VKU03_09430"
     CDS             complement(129..383)
                     /locus_tag="VKU03_09430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71527.1"
                     /translation="MTKTIFAALISASLLAAPTLAFADASCAAQAMDKKLAGAAKTSF
                     MTKCKKDAQAACDSQAAEKKLAGAAKTSFTKKCVSDATGG"
     gene            569..874
                     /locus_tag="VKU03_09435"
     CDS             569..874
                     /locus_tag="VKU03_09435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="accessory factor UbiK family protein"
                     /protein_id="HLJ71528.1"
                     /translation="MPQTSSRFFDDLSRLMSDAAGLAGGAKREAETLLRSQLERLMAG
                     MNLVSREEFEAARDMTVLARSENDKLREKVTGLEARLGSLEARVAHLEAEALKPPPA"
     gene            1077..1580
                     /locus_tag="VKU03_09440"
     CDS             1077..1580
                     /locus_tag="VKU03_09440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjN domain-containing protein"
                     /protein_id="HLJ71529.1"
                     /translation="MTPSLNVVADRPEHPLDVVERLASVNEWAFDRGDEDEISILVSG
                     AWTQYEVAFTWLPDIESLHVGCSFDLKAPPRRRAEVAALIQMINEQLWIGHFDLWTNE
                     DIVMFRHSLVLSGGAQPSDMQCGSIVQAALTACENYYQAFQFVLWAGKGAREALDIAM
                     FETQGHA"
     gene            1740..>2058
                     /locus_tag="VKU03_09445"
     CDS             1740..>2058
                     /locus_tag="VKU03_09445"
                     /inference="COORDINATES: protein motif:HMM:NF015747.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-binding domain-containing protein"
                     /protein_id="HLJ71530.1"
                     /translation="MTAWPDSLALVGAGKMGGAMLRGWLAGGLAADRVAILDPAPSMD
                     ILDLVRDRGLALPPPPGFKPEVVVLAVKPQTLDSAAPAIAELAHDGALVVSIIAGKRI
                     ADLQ"
BASE COUNT          322 a          678 c          676 g          382 t
ORIGIN      
        1 aagacgagca cgcgaagcgc gccgatccgg cgggccctcg ctcagacgct caattctttt
       61 gggcggcgcg gcgccgcccc gccgcccttc tccccggaca gcagcgagga gaagagggcg
      121 ggaccatttt agccgccggt cgcgtcagaa acgcatttct tggtgaagct ggtcttggcc
      181 gcgccggcga gcttcttttc ggccgcctgg ctgtcgcagg ccgcctgcgc gtccttcttg
      241 catttcgtca tgaagctggt cttggcggcg ccggccagtt tcttatccat cgcctgggcg
      301 gcgcaggacg cgtcggcgaa agcgagggtg ggcgcggcga gcaggctcgc ggaaatcagc
      361 gcggcgaaaa tcgtcttggt catcggaatc ctcctcgtga attcgcctgt cgaaggcgct
      421 cggctattat cggcgggcgc gccggcgacc acaatcccct tcaagggaac gttaaagaag
      481 cccgcccgcc cgggccgccg cctcttgcat cgccccgcct tgggctccaa atcttttttc
      541 acggccgtcc cggccgcggg agttcgtcat gccgcaaact tcgagccgat ttttcgacga
      601 tctgtcgcgt ctgatgtcgg acgcggccgg ccttgccggc ggcgcaaagc gcgaggccga
      661 gacgctgttg cgcagtcagc tcgagcggct gatggccggc atgaatttgg tttcgcgcga
      721 ggagttcgag gcggcccgcg acatgaccgt cctcgcccgg agcgagaacg acaagctgcg
      781 cgaaaaagtc acgggattgg aggccaggct cggctctttg gaggcgcgcg tcgcgcatct
      841 cgaggcggaa gcgctcaaac cgccacccgc ttgattcgtc ttagtgattc tgtggaagcc
      901 ggtttccgga cggtttacga attttcgcga atccgcgatg tcttcggcca tgttcgaggg
      961 actctgaggc gtctatagtc gcctcagggt gattcggcct ttgcgccgcg cgaccctcgg
     1021 acgtgtgaaa gcgtgccttg agagtcctgc tcaaaaccgc agccgaaggg tcaggaatga
     1081 cgccgagcct gaatgtcgtc gccgatcgtc ccgaacatcc cctcgacgtg gtcgaacgat
     1141 tggcgtccgt gaacgaatgg gccttcgacc ggggcgatga ggatgaaatc tcgatcctgg
     1201 tctccggcgc ctggacgcaa tacgaggtcg ccttcacctg gctgccggac atcgaatcgc
     1261 tgcatgtcgg ctgctccttc gatcttaagg cgccgccacg ccgccgggcc gaggtcgccg
     1321 ccctgatcca gatgatcaac gagcaattgt ggatcggcca tttcgacctg tggaccaacg
     1381 aggacatcgt catgttccgc cattcgctgg tcctttccgg cggcgcccag ccgagcgaca
     1441 tgcaatgcgg ctcgatcgtc caggccgcgc tcaccgcttg cgagaattac tatcaggcgt
     1501 tccaattcgt cctgtgggcg ggcaagggcg cgcgcgaggc gctcgacatc gccatgttcg
     1561 agacgcaggg ccacgcgtaa agagccatcg ggcgcattga tgatcgcggc cgcgactcac
     1621 ccttatccgg cgcttcgccc cgccttctcc cgcaagcggg ctgtagccgc ggtcggcgat
     1681 tgcggctgca acgcgacggc tgatgacagg cccggcgcgc gacgccgagg ctcggcggga
     1741 tgaccgcgtg gcctgattcc ctggcgcttg tcggcgccgg caaaatgggc ggggcgatgc
     1801 tgcgcggctg gctcgccggc ggcctggcgg cggaccgcgt cgccatcctc gatccggccc
     1861 cctccatgga cattctcgac cttgtccgcg atcgcggcct ggcgctgccg ccgccgcccg
     1921 gcttcaagcc ggaggtcgtc gtgctcgcgg tcaagccgca gacgcttgat tcggcggcgc
     1981 cggcgattgc cgagctcgcg catgacggcg cgctcgtcgt ctcgatcatc gccggaaaac
     2041 ggatcgccga tctccaag
//
LOCUS       DATGJW010000311         5424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1408318, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000311 DATGJW010000000
VERSION     DATGJW010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5424
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1408318"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(261..1151)
                     /gene="bla"
                     /locus_tag="VKU03_09450"
     CDS             complement(261..1151)
                     /gene="bla"
                     /locus_tag="VKU03_09450"
                     /EC_number="3.5.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473172.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008800 - beta-lactamase activity
                     [Evidence IEA];
                     GO_process: GO:0030655 - beta-lactam antibiotic catabolic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class A beta-lactamase"
                     /protein_id="HLJ71531.1"
                     /translation="MLTRRRFAQASFASVVAGPALGRAGFALAKDAFAPLAESFARIE
                     AESGGRLGVFALDTQSGASAGHRAAERFPMCSTFKLLAAAAILAKVDSGKEQLGRRIA
                     VKPSDILSYAPITKQHAGGEMSVGELCEAAITLSDNTAANLLVASLGGPQAVTQFARS
                     LGDEFTRLDRVEPDLNSSEPGDPRDTTLPSAMAKNLAKLATGAALAPASRDQLIGWLV
                     ACQTGGAKLRAGLPKAWRVGDKTGAGGHGSSNDVAVIWPAGRPPIVIASYLTETTASD
                     EKKNAAHAAVARAVADALTS"
     gene            1412..2179
                     /locus_tag="VKU03_09455"
     CDS             1412..2179
                     /locus_tag="VKU03_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ71532.1"
                     /translation="MKEFSSAGIRIAYADFPAKGEDRGEPILLIHGFASSHVFNWVNP
                     SWIKTLTEAGRRTIALDNRGHGQSEKLYRPEDYASDVMAEDARRLLDELALERADVMG
                     YSMGARIGAFLALAHPGRARSLILGGLGDHLVEGVGLPLGIADAMEAASIDELIDPQQ
                     RTFRAFADQTKSDRAALAACIRGSRQTLRPEEVAAIRCPTLVAVGTRDPVAGDPHRLA
                     ALFPKGEALDIPNRDHMLAVGDKAFKAGALAFLARRP"
     gene            2209..2952
                     /locus_tag="VKU03_09460"
     CDS             2209..2952
                     /locus_tag="VKU03_09460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLJ71533.1"
                     /translation="MPLFVLFVASLWAGAQNALAGGGSFLTLPALIAAGMDARAANIT
                     STVALFPAQIVTGLTGRRGASGLPDLSLKSLFAISLVGGGVGGAILLATPASFFARLV
                     PWLVLFATAVFAWGSFFRRPPAAGQSHMPQGAAALAQFLIAIYGGYFGGGIGFLMMAA
                     LTTAGQNVRMAGATKNILAGVINAAAVAIFVFSPDIHWLQAGIAALGASIGGVAGALM
                     LQKVNEKALRIGIVAIGLILTVGLFLRAP"
     gene            3090..3920
                     /gene="cysE"
                     /locus_tag="VKU03_09465"
     CDS             3090..3920
                     /gene="cysE"
                     /locus_tag="VKU03_09465"
                     /EC_number="2.3.1.30"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009001 - serine O-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006535 - cysteine biosynthetic process
                     from serine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine O-acetyltransferase"
                     /protein_id="HLJ71534.1"
                     /translation="MAQTQIARVVGLGRNDPLFARICAEAEEAARREPELAAFLMMNI
                     LHHETFEGAVIHRVAARLDHADAPAALIRQTYADFVSRDTSLGEAFRADLLAVADRDP
                     ACHRMMEPLLYFKGFHALQAYRLSHALLRAGRKDFALYLQSRASAVFQADINPAAKIG
                     KGVFLDHATGLVVGETCVIEDDCSILQGVTLGGTGKETGDRHPKIRWGVLIGAGAKIL
                     GNIEVGHCARVAAGSVVLAPVPHNKTVAGVPARIVGEAGCAEPARSMNQIIAEKLVEP
                     "
     gene            4183..4578
                     /locus_tag="VKU03_09470"
     CDS             4183..4578
                     /locus_tag="VKU03_09470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3126 family protein"
                     /protein_id="HLJ71535.1"
                     /translation="MEKSEILKLQAHLRRCFGAPGVKVMPSSKAQDAADVQLGERKLG
                     VITVDDEDGDRSFSFEMKAPVQRHVLQDYFRRLFENDKLKILARGRKTDSVEIANGDD
                     FLGVLSADDAKGTTFTLQMAILDFDLEDL"
     gene            4626..5042
                     /locus_tag="VKU03_09475"
     CDS             4626..5042
                     /locus_tag="VKU03_09475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71536.1"
                     /translation="MALHILAAAIDFALYAILLVFAFRYLLTGRYMPAHAQAAGHDWG
                     SLDPKTQAIASAMARVVGAGMLTTAVTGGLITLGAAITGLAWLKYLAPVPALIFCAPT
                     LHASYVLWRSAGAATPMAPALAGVVLAIAGFVLWRM"
     gene            5103..>5424
                     /locus_tag="VKU03_09480"
     CDS             5103..>5424
                     /locus_tag="VKU03_09480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF427 domain-containing protein"
                     /protein_id="HLJ71537.1"
                     /translation="MPDEHRIVVAPASRRVRVIWRGHIIADSPGALSLKEHVYRPVFY
                     IPREDVAMMFLQRTASHTTCPYKGEASYYSLAADGEVEQDAVWSYETPKPLVAPIKDH
                     LAFYP"
BASE COUNT          847 a         1800 c         1831 g          946 t
ORIGIN      
        1 aactgttgag aaaaagcagc gggcggccgc gcccgctctc gacccaatag agccgcgttc
       61 cgtcctgcgc ttcgatatat cgcgtctcgt tgcttcggcg cggcgcggaa gcgcgcgcgg
      121 cgaaaggggc ggcgccggcg gcgagcgccg cggacggttc ttgcatgcgc atcttagtct
      181 ctccatgagc gttgcggagg gactatgcgc agcggcgcgg cgcgaagcga ttacgcccga
      241 ggtaaaggcg ggcctcttcg ttagctcgtc aaagcgtccg cgaccgcccg ggcgacggcg
      301 gcgtgcgccg cgttcttctt ctcgtccgac gctgtagtct cggtcaggta ggaggcgatc
      361 acgatgggcg gccggcccgc cggccagatc acggcgacat cgttgctcga tccgtggccg
      421 cccgcgccgg tcttgtcgcc gacccgccag gctttcggca ggccggcgcg gagcttggcg
      481 ccgccggtct ggcaggcgac gagccatccg atgagctgat cgcgcgaagc cggggcgagg
      541 gcggcgccgg tcgcgagctt ggcgaggttc ttggccatgg cggaggggag ggtcgtgtcg
      601 cgcggatcgc cgggctcgct gctgttgagg tccggctcga cgcggtcgag ccttgtgaat
      661 tcgtctccga gcgaacgggc gaattgcgtc accgcctgcg gcccgccgag gctcgcgacc
      721 agcagattgg cggccgtatt gtcggaaagc gtgatcgccg cctcgcagag ctcgccgacc
      781 gacatctcgc cgccggcatg ctgcttcgtg atcggcgcgt aagagagaat gtcgctcggc
      841 ttgaccgcga tgcgccgccc gagttgctcc ttgccggaat cgaccttggc caagatcgcg
      901 gcggccgcaa gcagcttgaa ggtcgagcac atcggaaagc gctcggcggc gcgatggccc
      961 gccgaggcgc cgctctgcgt atcgagggcg aacacgccga gccggccgcc gctctcagcc
     1021 tcgatcctgg cgaaagactc ggcgagcggc gcgaaagcgt ccttggcgag ggcgaagccg
     1081 gctcgcccga gcgccgggcc ggcgacgacc gaggcgaaag aggcttgcgc aaagcggcgg
     1141 cgagtgagca tgcggggaaa ggctcctgct gggggaaatc aaaaggcgag agcggagaaa
     1201 cggacaaagg cgagggcggg tctcttagaa tcgggacgtg accgaaaaaa ggcggccgac
     1261 gagacggccc gcgggaggta cccgcccggc gcaatccgcc cgcgcgatca tccgtactct
     1321 gcgcgcgctg gcgcttgcgc cgcagatcgg ccgctacagc ttggcgcctg cggatggtag
     1381 agtgccggtg cggccagacg aggtgaagag cgtgaaggaa ttttcatctg cgggaattcg
     1441 catcgcctat gccgattttc ccgccaaggg cgaggatcgc ggcgagccca tcctgctcat
     1501 ccacggcttt gcttcgagcc atgtcttcaa ctgggtcaat ccgagctgga tcaagacgct
     1561 gaccgaggcg ggccgccgca cgatcgcgct cgacaatcgc ggccacggcc aaagcgagaa
     1621 gctgtaccgg ccggaggatt acgccagcga cgtcatggcc gaggacgcgc ggcggctgct
     1681 cgacgaactg gccctcgaac gcgccgacgt catgggctat tcgatgggcg cgcgcatcgg
     1741 cgcttttctc gcgctcgccc atcccggccg cgcgcgctcg ctgatcctcg gcggcctcgg
     1801 cgatcatctt gtcgagggcg tcggtctgcc gctcggcatc gccgatgcga tggaggccgc
     1861 gtcgatcgac gaattgatcg acccgcagca gcgaaccttc cgcgctttcg ccgatcagac
     1921 caagagcgat cgcgccgcgc tcgccgcctg catccgcggc tcgcgccaga ccttgcggcc
     1981 ggaggaggtc gcggcgatcc gttgtccaac cttggtcgcc gtcggaacgc gcgatccggt
     2041 ggctggcgat ccgcatcggc tcgcggcttt gtttccgaaa ggcgaggcgc tggacattcc
     2101 caaccgcgat cacatgctgg ctgtcggcga caaggccttc aaagccggcg cgctcgcctt
     2161 cctggcgcgc cggccatagg cgcgtcattc ccgggaatga catggatgct gccgctcttt
     2221 gtcttattcg tcgcctcgct ttgggcgggc gcgcagaacg cgctggccgg cggcggctct
     2281 ttcttgacct tgccggcgct gatcgccgcc ggcatggatg cgcgcgcggc gaacatcacg
     2341 tcgaccgtcg cgctgtttcc cgcccagatc gtcaccggac tgaccggacg gcgcggcgct
     2401 tccggcctgc cggatttgtc gctcaaaagc ctcttcgcaa tcagcctggt cggcggcggc
     2461 gttggcgggg cgatcctctt ggcgacgccg gcgagctttt tcgcccgcct cgtgccctgg
     2521 ctcgtccttt tcgccacggc ggtcttcgcc tgggggagct tctttcgccg gccgcccgcc
     2581 gccggccaaa gccatatgcc gcaaggcgcg gcggccctgg cgcaattcct gattgcgatc
     2641 tatggcggct atttcggcgg cggcatcggc tttctgatga tggcggcttt gaccacggcc
     2701 gggcagaacg tccgcatggc cggcgcgacc aagaacatcc tcgccggcgt gatcaatgcg
     2761 gcggcggtgg cgattttcgt cttctcgccc gacattcact ggctgcaagc cggaatcgcc
     2821 gcgttaggcg cctcaatcgg cggcgtggcc ggcgcgctga tgctgcagaa ggttaacgaa
     2881 aaggctttgc gcatcggaat cgtcgcgatc ggcctcattt tgacggttgg cctctttctg
     2941 cgcgcgccgt gagcctctgt agcggtcgtc gaccgccgct gcagcgtcga ccggccacgg
     3001 ccggaggctg acggcgcgat ccagaccgcc tatatttcgg agacggcttc gtgtaagaga
     3061 gccgcccgtt ttgcggagag gagagcgtca tggctcagac ccagatcgcg cgcgttgtcg
     3121 gcctcggccg caatgatcct ttattcgcgc gcatctgcgc cgaggcggag gaggcggcgc
     3181 ggcgcgagcc cgaactcgcc gccttcctga tgatgaacat cctccatcac gagacgttcg
     3241 agggcgcggt cattcatcgc gtcgccgccc ggctcgatca cgccgacgcg ccggccgcgt
     3301 tgatccgcca gacctacgcc gatttcgtct cgcgcgatac ttcgctcggc gaagccttca
     3361 gggccgacct tctcgccgtc gccgaccgcg atccggcgtg ccatcggatg atggagccgc
     3421 tgctctattt caaaggcttc catgcgcttc aggcttatcg cctgtcgcac gctttgctgc
     3481 gcgcgggccg caaggacttc gccctttacc tgcaaagccg cgcctcggcg gtgttccagg
     3541 ccgacatcaa tccggcggcc aagatcggca agggcgtttt cctcgaccat gcgacgggcc
     3601 tcgtcgtcgg cgagacctgc gttatcgagg acgattgctc gattctgcaa ggggtgacgc
     3661 ttggcggcac cggcaaggag accggcgacc gccatccgaa gatccgctgg ggcgtgctga
     3721 tcggcgccgg cgcgaaaatc ctcggcaata tcgaggtcgg ccattgcgcg cgggtcgcgg
     3781 cgggctcggt cgtgcttgcc ccggtgccgc acaataagac cgtcgccggc gtgccggcgc
     3841 gcatcgtcgg cgaagccggc tgcgccgagc cggcgcgcag catgaaccag atcatcgcgg
     3901 aaaagctggt cgagccttag ccgacgactg cgggcgaccc tcaccccaac cctctcccgc
     3961 aagcgggaga gggggcgcgc tgagcgcttc atcagccgca gtgcgttagc tggtcggcaa
     4021 actcggctac ccgccccttc tcccgcttgc gggagaaggt ggcccgaagg gccggatgag
     4081 ggtgagccgc agcctgaaag gtcagccggc ttgccgcctg cctcctatcg tggcaagcca
     4141 cggccttgcg gagcgcaacg cgccaggaag agcacgaggc caatggaaaa gagcgagatt
     4201 ctcaaattgc aggcgcatct gcgccgctgc ttcggcgcgc ccggcgtcaa ggtcatgccg
     4261 agctcgaaag cacaggacgc cgccgacgtg caactcggcg agcgcaagct cggcgtgatc
     4321 acggtcgacg acgaggacgg cgaccgctcc ttctcgttcg agatgaaggc gccagtccag
     4381 cgtcatgttt tacaagatta ttttcgccgc ctgttcgaga acgacaagct caaaatcctg
     4441 gccaggggac gcaagaccga ttccgtcgag atcgccaatg gcgacgattt cctcggcgtt
     4501 ctgtcggccg acgacgccaa ggggacgacc ttcaccttgc agatggcgat cctcgatttc
     4561 gatctggaag acttgtgaaa cgaagcgctt gcgggccaga atgaacgctt ccaggcggag
     4621 ctgcgatggc cctccatatt ctcgccgcgg cgatcgattt cgcgctctat gcgatcctgc
     4681 tggttttcgc ctttcgctat ctcctgaccg gccgctacat gccggcccac gcccaggcgg
     4741 cgggccatga ttggggctcg ctcgatccca agacccaggc catcgcctcg gccatggcgc
     4801 gggtggtcgg cgccggcatg ctgaccacgg ctgtgaccgg cggcctgatc acgctcggcg
     4861 cggcgatcac cggccttgcc tggctgaaat atctcgcccc cgtgccggcc ctcatctttt
     4921 gcgcgccgac cttgcacgcc tcctatgtcc tctggcggtc ggcgggcgcc gccaccccga
     4981 tggccccggc gcttgccggc gtcgttctcg cgatcgcggg tttcgttttg tggcggatgt
     5041 gaggcgaacc tttgcggccg gcgggacgtt ggaacgcggc agcggaacag gagcaaaccg
     5101 ccatgccgga tgagcacaga atcgtcgtcg cccccgcgtc gcgccgggtt cgcgtcatat
     5161 ggcgcggcca tatcatcgcg gattcgcccg gggccctgtc tttgaaggag catgtctatc
     5221 ggccggtctt ctacatcccg cgcgaagacg tggcgatgat gtttctccag cgcacggcct
     5281 cgcacacgac ctgcccctat aagggcgagg cgagctatta ttcgctggcg gccgatggcg
     5341 aagttgagca ggacgccgtg tggagctatg agacgccgaa gccgctcgtc gcgccgatca
     5401 aggaccatct cgccttttat ccgg
//
LOCUS       DATGJW010000312        15615 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1509619, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000312 DATGJW010000000
VERSION     DATGJW010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 15615)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 15615)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..15615
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1509619"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..267
                     /locus_tag="VKU03_09485"
     CDS             <1..267
                     /locus_tag="VKU03_09485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71538.1"
                     /translation="RYPLDIGGLSYGFTFGASETHFHGEVSHIRRPSDVSGVYAAGNV
                     GAAVGRGAQAIVLTNQNGAVLTLAGNSVGAIVSADLNGLVLTVK"
     gene            complement(303..1289)
                     /locus_tag="VKU03_09490"
     CDS             complement(303..1289)
                     /locus_tag="VKU03_09490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008533661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HLJ71539.1"
                     /translation="MSLKILFIGGAGNISLPCVVEAVAAGHEVSVFNRGATAADLPPS
                     VRAIRGDINDAAAYRALGKSGFDVVCQFIVFTPAQMAADIAAFSSGVGQYVFISSASV
                     YEKPPRGFVITEKTPTVNPYWRYSQDKIACEAMLKAAKDLAWTIVRPSHTVRTMLPTM
                     VNEGDAVAHRMLAGKPVIVAGDGATPWTLTRCADFAKPFVRLFGKTEALGEDFHITSD
                     HAYRWDAIYEAIAAGLGVKADIVHAPTEALIRYIPDWEGPLLGDKTWTALFDNSKVKR
                     VAGDFSCEKDLGGVLKEPIASAKARIKASGPKTSKHDATIDKIARELRALGT"
     gene            1472..2194
                     /locus_tag="VKU03_09495"
     CDS             1472..2194
                     /locus_tag="VKU03_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1223 domain-containing protein"
                     /protein_id="HLJ71540.1"
                     /translation="MTIAAAPSGIIGTVAAVGALALASLSQPAAAENRLTVVELFTSQ
                     GCSSCPPANANLATISGLPGVLALSFSVTYWDRLGWKDTLGRPDYTARQEAYEGPLGE
                     EGPFTPQMVIDGRESFVGNDLSDLERRLAAARQEQASAGPSLAVKDRIARIGAGPAPR
                     GGADIWLVRYDPRRVDVPVARGENAGRTLPHKNVVRDLSLIGRWGGEPVTIRTPQAEG
                     GLRTAILVQAHDGGAILAATTD"
     gene            2306..2569
                     /locus_tag="VKU03_09500"
     CDS             2306..2569
                     /locus_tag="VKU03_09500"
                     /inference="COORDINATES: protein motif:HMM:TIGR02953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide MXKDX repeat protein"
                     /protein_id="HLJ71541.1"
                     /translation="MTRIHILARPVLLALALSLGGLAVAQADDAMKSDAMKGDSMKSG
                     AMKKNHAMKGDAMKSNAMKGDAMNANAMKKDGAMKSDEATPKN"
     gene            complement(2626..3084)
                     /locus_tag="VKU03_09505"
     CDS             complement(2626..3084)
                     /locus_tag="VKU03_09505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011085288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLJ71542.1"
                     /translation="MPGPSVAYSSTSAHAAPLAPAPINPDWILEGAPEAQNAILSRSA
                     DGTACTIMWSCTAGKFRWLYDFDETIHFLEGWVAIDDGSGPRRLGPGDVVFFPAGTSA
                     VWTVETYVRKLAFCRKALPAPLGAAVNMLRAMKNAMRGAPSEGASLMKSA"
     gene            complement(3359..3781)
                     /gene="ndk"
                     /locus_tag="VKU03_09510"
     CDS             complement(3359..3781)
                     /gene="ndk"
                     /locus_tag="VKU03_09510"
                     /EC_number="2.7.4.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004550 - nucleoside diphosphate kinase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside-diphosphate kinase"
                     /protein_id="HLJ71543.1"
                     /translation="MAVQRTFSILKPDVTRRNLTGAVNAKIEAAGLSIVAQKRVLMSR
                     REAETFYAVHKERPFFGELVEFMTSGPVVVQVLEGENAIQRYRDVMGATNPEKAAAGT
                     IRKEFAVSMGENSVHGSDAPETAAIEIAQFFSGNEIVG"
     gene            complement(3875..4273)
                     /locus_tag="VKU03_09515"
     CDS             complement(3875..4273)
                     /locus_tag="VKU03_09515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007796356.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HLJ71544.1"
                     /translation="MRRIGFLLGAWLALALPAFGEDCPDQNQQGLNACANAAYKKADD
                     ALNGVYRETMRRLKDDAAAAKLLVAAQKAWIAFRDAECAFAASENAGGSIYPMVYAEC
                     LERLTKARTSALRLYLKCGEGDLGCPAPGK"
     gene            4370..5116
                     /locus_tag="VKU03_09520"
     CDS             4370..5116
                     /locus_tag="VKU03_09520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008125558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ71545.1"
                     /translation="MSKTILVTGGSRGIGRAVCLLAARRGWSVGVNYVHDKEAAEAIV
                     ASAAREGARALAIPGDVAEESDVIAMFEKTAETLGPLDGVVVNAGIVAPAARLIEMSV
                     ERMRRIFDVNTLGAYVTAREAARRMMTSQGGRGGSIVLMSSAAARLGSPDLYVDYAGS
                     KGAIDTLTIGLAQELGREGVRVNAIRPGMIETDIHASGGEPGRAHRVGATAPMGRPGR
                     AEEIAEAVVWLLSDAASYTTGAILDVSGGR"
     gene            complement(5215..5586)
                     /locus_tag="VKU03_09525"
     CDS             complement(5215..5586)
                     /locus_tag="VKU03_09525"
                     /inference="COORDINATES: protein motif:HMM:NF013304.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MAPEG family protein"
                     /protein_id="HLJ71546.1"
                     /translation="MTVAFWCVLIAGLLPYVVFGPAATKLDIKTPRRLVADLEGLPAR
                     AHGAHLNHFEAFPFFAAAVIIAHVVSGPSALVNWLAVAFIVVRVFYTAMYLTDRQPLR
                     SSSFFVGLLIAIAIFISPAFH"
     gene            5897..8641
                     /gene="gyrA"
                     /locus_tag="VKU03_09530"
     CDS             5897..8641
                     /gene="gyrA"
                     /locus_tag="VKU03_09530"
                     /EC_number="5.6.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006259 - DNA metabolic process [Evidence
                     IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HLJ71547.1"
                     /translation="MSDSNNERPAGGGPSDIRPVSITDEMKRSYLDYAMSVIVSRALP
                     DARDGLKPVHRRILFSMSEQNFTPDRPYNKSSRTVGDVIGRYHPHGDLAVYEALVRLA
                     QDFSMRAPLIDGQGNFGSVDGDPPAAQRYTEARLERVAMALLEDLDKDTVDFRPNYDE
                     KEFEPVVLPARFPNLLVNGAGGIAVGMATNIPPHNLGETIDAVLAVMERPDISLDDLI
                     EIIPGPDFPTGGAILGRGGIRAAYATGRGSIIMRAKARIEEIRKDREAIIVDEIPYQV
                     NKASLVERIADLVREKKIEGIGDLRDESDRDGMRIVIELKRDAVADVVLNQLWRFTAM
                     QTSFGCNMIALNGGKPELLTLKDMLQAFVDFREEVVTRRTKHLLAKARDNAHLQVGLA
                     IAVANIDEVIRLIRTSPDAAAAREALMERTWPARDMAPLVALIADPRHALAEDGTCRL
                     SDAQARAILDLRLQRLTALGRDEIAAALNKLAEEIADYLDILSSRARVRSIIHDEIVA
                     VKEAFATPRRTEILDSDSDMEDEDLIQREDMVVLVSHAGYIKRVPLSTYRAQRRGGKG
                     RSGMQTREEDFVARLFIASTHQPVLYFSSKGKVYKEKVWRLPLAPPQARGKALVNLLP
                     LEEGERITTIMPLPENEKDWENLDVMFATTRGTVRRNKLSDFAQVNRSGKIAMKLDEG
                     EGIVDVQTCSERDDVLLTTAQGQCIRFAVDDVRVFKGRDSMGVRGIALAEGDGVISLA
                     ILRQFEASAEERAAYLKMRRAVAGEVGENGNGNGEEASDGDAEAVAETALSQERYAEM
                     SAAEQVILTISENGYGKRSSSFEYRATGRGGKGIVAMAVNQRNGKLVASFPVEHDDEI
                     MLVTNGGQLIRCPVEDIRIAGRATQGVIVFDTADAERVVSVERISEPEEEPEAEENGG
                     "
     gene            complement(8726..9562)
                     /locus_tag="VKU03_09535"
     CDS             complement(8726..9562)
                     /locus_tag="VKU03_09535"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent methyltransferase"
                     /protein_id="HLJ71548.1"
                     /translation="MQAQASLTAKGAATHRAVHQLLEGGRIFSDPLAVAILGEDPDAL
                     RREAEDDPARRFMRLFIAARSRLAEDRLAEAVARHVRQLVVLGAGLDTFGLRNPHRAK
                     GLKVFEVDHPATQAWKRERIAAAMTGEPPALSFVAVDFEKQSFIDELAKAGFDRQAPA
                     FFIWLGVTPYLTRDAIVSTLTAVARASAEIVFDYGEPLDAFSGARRAAMEAFQARVAA
                     IGEPLLSRFRPADMAALLRETGFAHFDDFGPHEMAAYLGAPSPPPAGTPGGHVVHARS
                     AV"
     gene            complement(9630..10130)
                     /gene="coaD"
                     /locus_tag="VKU03_09540"
     CDS             complement(9630..10130)
                     /gene="coaD"
                     /locus_tag="VKU03_09540"
                     /EC_number="2.7.7.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004595 - pantetheine-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pantetheine-phosphate adenylyltransferase"
                     /protein_id="HLJ71549.1"
                     /translation="MARVIFYAGSFDPLTNGHLDVIGRAARLCDKLVVGVGAHASKVA
                     LLSTDERVRLIGEAAAPLLRSGGGALETIVFSGLAVEAAAAAGASAIVRGLRDATDFD
                     YEMQLAGMNADMAPKLQTIFLPASRNVRHITATLVRQIASMGGDVSPFVPPNVAAALK
                     GKFAKG"
     gene            complement(10178..10546)
                     /locus_tag="VKU03_09545"
     CDS             complement(10178..10546)
                     /locus_tag="VKU03_09545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906860.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000917 - division septum assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein ZapA"
                     /protein_id="HLJ71550.1"
                     /translation="MAHVTVMIGGRSYRLACNEGEEPHLEGLARQIDSKIAEIRGQVG
                     EIGDQRLVVMAALTIADELFEARRKVSELERKGSERDDVAEAVRREADEWAAAVAEAL
                     SDASRRIEDVAAALAAGGKG"
     gene            complement(10650..10940)
                     /locus_tag="VKU03_09550"
     CDS             complement(10650..10940)
                     /locus_tag="VKU03_09550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4164 family protein"
                     /protein_id="HLJ71551.1"
                     /translation="MSADLLEPSFKRLRAALDLLEAAIERRARQEGARGDTQEELALM
                     QDDRARLAVELDGALDRNRGLLAANMEAARRVEKASAAIRSILERHRVPEPE"
     gene            11177..13153
                     /gene="tkt"
                     /locus_tag="VKU03_09555"
     CDS             11177..13153
                     /gene="tkt"
                     /locus_tag="VKU03_09555"
                     /EC_number="2.2.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004802 - transketolase activity [Evidence
                     IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HLJ71552.1"
                     /translation="MAANVTHDQMANAIRALSMDAVEKANSGHPGMPMGMADVATVLF
                     RDVLKFDAADPAWPDRDRFVLSAGHGSMLLYSLFYLTGTGGMAIEDLKQFRQVGSKTP
                     GHPEYRHTIGVETTTGPLGQGLATSIGMALAETMLAAEFGDLVDHHTFVIASDGDLEE
                     GVSHEAISIAGHLRLNKLIVLWDNNSISIDGKTSLADSVDHVARFKAAGWRAAHIDGH
                     DPKQVLRALRAAKRSDRPTMIACKTTIGFGLPTKAGTNKAHGEAPGAAEVAGARVNLH
                     WPYEPFVVPDDILEAWRAVGRRGKSKRNRWRRLLKSKPPEIRAEFERRLAGELPKGLD
                     EVIDAYKRKLAADAPEIATRKAGEAALKVIAPAAPELITGSADLTGSNNTKVEATREI
                     TPEDKSGRYIHWGIREHGMIAACNGLALHGGFIPSGASFLVFTDYCRPSLRLAALMGI
                     RVCHVFTHDSIGLGEDGPTHQPVEHLASLRAMPNMFTFRPADSVETVECWQAALKMRR
                     SPSILALTRQNLPALRRTHVAENLSARGAYEISPAAGKAAVSIFASGSEVSLAVAAQK
                     LLAEKGVVARVVSVPCMDLFFEQKEAYRQSVIGDAPVKVGVEAAVRQGWDAIIGDGPF
                     IGMKGFGESGPYKAVYEHFGITPQAVAEAALARL"
     gene            13307..14314
                     /gene="gap"
                     /locus_tag="VKU03_09560"
     CDS             13307..14314
                     /gene="gap"
                     /locus_tag="VKU03_09560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011443333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HLJ71553.1"
                     /translation="MAVRVAINGFGRIGRNVLRAIFESGRKDIEVVAINDLGPVETNA
                     HLLRFDSVHGRFPHEVKVEGDTINIGVGKIKVTAIKDPAQLPHKELGVEIALECTGIF
                     TARDKAAAHLTAGAKRVIVSAPSDGADLTVVYGVNHEKLTKEHLVISNASCTTNCLAP
                     VAKVLNDTIGIDHGFMTTIHSYTGDQPTLDTLHKDLYRGRAAALSMIPTTTGAAKAVG
                     LVLPELKGKLDGSAIRVPTPNVSVVDLKFVAKRPTSKDEISTAIKNAAAQQLKGILGY
                     TDQPNVSADFNHDSRSSVFHLDQTKVIDGTFVRVLSWYDNEWGFSNRMADTAVAMSKV
                     I"
     gene            14397..15599
                     /locus_tag="VKU03_09565"
     CDS             14397..15599
                     /locus_tag="VKU03_09565"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799008.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HLJ71554.1"
                     /translation="MSSSFRTLDDASLAGKRVLLRVDLNVPMEHGRVTDATRIERVLP
                     TIHEISRKRGKVILLAHFGRPKGGPDDANSLRPVAAAVEALLKQPVAFAPDCIGEAAT
                     KAIAAMKDGDVLLLENTRFHKGEEKNDPAFVAELAKLGELYVNDAFSAAHRAHASTEG
                     LAHRLPAYAGRAMQAELEALGKALEAPARPLVAIVGGAKVSTKLDLLGNLVTKVDALV
                     IGGGMANTFLHAQGYPVGKSLCEKDLADTARAIMAKAQESRCAIILPLDAIVAFHFAA
                     NAPAHDYGIDAIPPEGMMLDIGQLSVDRIKASINEAGTLVWNGPVGAFELAGFEHGTM
                     ALARHVAERTKAHKLVSVAGGGDTVAALNAAGVVDQFTYVSTAGGAFLEWMEGKALPG
                     VEALRRPA"
BASE COUNT         2758 a         5149 c         5157 g         2551 t
ORIGIN      
        1 cggtacccgc tcgacatcgg cggtctcagc tatggcttca ccttcggcgc ctccgagacg
       61 catttccatg gcgaggtgag ccacatccgg cggccgtcgg acgtttccgg cgtctatgcg
      121 gcggggaatg tcggcgcggc cgtcgggcga ggcgcgcagg cgatcgtgct gaccaaccag
      181 aacggcgcgg tgctcaccct tgccggcaat tcagtcggcg cgatcgtcag cgccgacctc
      241 aacggcttgg tgctcacggt caagtaggcg agacgccgct ttttgcgagc tggcgcgatt
      301 ggctacgtgc cgagggcgcg cagctcccgc gcgattttgt cgatcgtcgc gtcatgcttg
      361 ctcgtcttcg ggccgctcgc cttgatccgc gccttggcgc tggcgatcgg ctctttcagc
      421 actccgccca ggtccttctc gcatgaaaag tcgccggcga cgcgcttgac cttggaatta
      481 tcgaacagcg ccgtccaggt cttgtcgccc agaagcgggc cttcccagtc cgggatatag
      541 cggatgagcg cctcggtcgg cgcgtgcacg atgtccgcct tgacgccgag gccggcggcg
      601 atcgcctcgt agatggcgtc ccacctatag gcgtggtccg aggtgatgtg aaagtcctcg
      661 ccgagcgctt ccgtcttgcc gaaaagcctg acgaacggct tggcgaaatc cgcgcacctc
      721 gtcagcgtcc agggcgtcgc gccgtcgccg gcgacgatca ccggcttgcc cgcaagcatg
      781 cgatgggcga cggcgtcgcc ctcgttgacc atggtcggca gcatggtccg gaccgtgtgg
      841 ctcggccgca cgatcgtcca ggcgagatct ttggcggcct tcagcatcgc ctcgcaggcg
      901 atcttgtcct ggctgtagcg ccagtacgga ttcaccgtcg gcgtcttctc cgtgatgacg
      961 aagccgcgcg gcggcttttc atagaccgac gccgaggaga tgaaaacata ttggccgacg
     1021 ccgctggaaa aggcggcgat atccgccgcc atctgcgccg gcgtgaagac gatgaactgg
     1081 cagacgacgt cgaacccgct cttgccgagc gccctatacg cggccgcgtc attgatgtcg
     1141 ccgcggatcg cgcgcacgct tggcggcagg tccgcggcgg tcgcgccgcg attgaagacg
     1201 ctgacctcgt ggccggccgc aaccgcttcg acgacgcagg gcagcgaaat attgccggcg
     1261 ccgccgatga agagaatttt cagggacatg ggcgcggtcc ttcctcgacg caggccaagt
     1321 aggccgcctc ggcggcgaaa aaacaagcgc ctcgcccggc gacggcaggc gagccgcttc
     1381 aagccgccgc gatcacgaat ccgtgatcgc ggtaacgtcg cgccgatttt cccgaaagtc
     1441 ttgaagacaa gccgcaattt gagggacgag catgacgatc gccgcagcgc catccggcat
     1501 cataggtacg gttgccgccg tcggcgcgct cgcgctggcg agcctctcgc aacccgccgc
     1561 ggctgaaaac cgcctcaccg tcgtcgaact cttcaccagt cagggctgct cgtcctgtcc
     1621 gccggccaac gccaatcttg cgaccatcag cggactgccg ggcgtgctcg cactgagctt
     1681 cagcgtcacg tactgggatc ggctcggttg gaaggatacg ttaggccgcc cggactatac
     1741 cgcgcggcag gaggcctatg aggggccgct cggcgaagag gggccgttca cgccgcagat
     1801 ggtgatcgac ggccgcgaga gttttgtcgg caatgatctg agtgacctgg agcgccgcct
     1861 cgccgcggcg cgccaggagc aagcgagcgc cgggccgtcg ctcgccgtca aagaccgcat
     1921 cgcccggatc ggcgcgggcc ccgcgccgcg cggcggcgcc gatatttggc tcgtgcgcta
     1981 cgatccgcgg cgcgtcgacg tgccggtcgc gcgcggcgag aatgccggcc gcactttgcc
     2041 tcataagaac gtggtgcgcg atctatcgct gattgggcga tggggcggcg aacccgtgac
     2101 gatcaggacg ccgcaagccg aaggcgggct gcgcacggcg attctcgtcc aggcgcatga
     2161 cggcggcgcg attctcgccg cgacgaccga ttgaactttc accaaatccg gagcgagcga
     2221 aagctcgttc ggtactcgcg cgaccgatgg tcggccgcgc tttctgaagg ggccgcaggt
     2281 cccgttaaca aatgaggacg acgccatgac tcggattcac attctcgcgc gccccgtttt
     2341 gcttgccctg gcgctttcgt tgggcggcct cgccgtcgcg caggccgacg acgcgatgaa
     2401 gagcgacgcg atgaaaggcg attcgatgaa atcgggcgcg atgaagaaaa atcacgccat
     2461 gaagggcgat gcgatgaaga gcaacgccat gaagggcgac gcgatgaacg cgaatgcgat
     2521 gaagaaggac ggcgctatga aatccgacga ggccacgccg aagaactgat cttcggattg
     2581 cgaagcgccg cccgccgcgg cgtttcgctc cggtcgatca tctggtcacg ccgacttcat
     2641 cagcgacgcg ccttcgctcg gcgcgccgcg catcgcgttc ttcatcgcgc gcagcatgtt
     2701 gaccgccgcg ccgagcggcg ccggcagcgc cttgcggcag aaggcgagct tgcggacgta
     2761 agtttcgacc gtccagaccg cgctcgtccc ggccggaaaa aagacgacat cgccaggccc
     2821 gagccggcgc gggccggagc cgtcgtcgat cgcgacccag ccttccagga agtggatcgt
     2881 ctcgtcgaaa tcgtagagcc agcggaactt gccggccgtg caactccaca taatggtgca
     2941 ggcggtcccg tcggcgctgc gcgagagaat ggcgttttgc gcttccggcg cgccttcgag
     3001 aatccagtcc ggattgatcg gcgccggcgc aagcggcgcc gcatgcgctg aagtcgacga
     3061 ataagcgact gatggtccgg gcatgtctcc cctccaacca agcgcgacca gccggagggc
     3121 ggacttggct ttaaaaattg agggaagaaa ataacacgcg ggagagccgg attcttcgcc
     3181 gattcgccga taaggctaac gagtgattat tttgatcgcg gaatcggcgc gcgcatatcg
     3241 tacggagcca atcggcgttc tcagccgctc tgcggttgcg ctgagccatg gctcgcgagc
     3301 gagcgccgac gcggcctcgc cgcgagccgc ggagctttgt acttctcggc gctccccgct
     3361 atccgacgat ttcgttccct gagaagaatt gcgcgatctc gatcgccgcg gtctccggcg
     3421 cgtctgagcc atgcacggaa ttctcgccca tcgaaacggc gaattccttg cggatcgttc
     3481 ccgccgccgc cttttccggg ttcgtcgcgc ccatcacgtc gcgatagcgc tggatcgcat
     3541 tctcgccttc gagaacctgc accacgaccg gcccggacgt catgaactcg accagctcgc
     3601 cgaaaaacgg ccgctccttg tgcaccgcat agaacgtctc ggcctcgcgg cggctcatca
     3661 gcacgcgctt ctgcgcgacg atgctgaggc ccgcggcctc gatcttggcg ttgacggcgc
     3721 cggtgagatt gcggcgggtg acgtcgggct tgaggatcga gaaggtgcgc tgaacggcca
     3781 tggaacgatg tttccgatgt ctgggagaat ggcgccggcc ttatagaggc ggcggccgcg
     3841 cccggcaaga cgacgaaagc ttgagcgcgc cggcctattt gcccggcgcg gggcagccga
     3901 gatcgccttc gccgcatttc aggtaaaggc gcagcgcgct cgtccgcgcc ttggtcaggc
     3961 gctccaggca ttcggcgtag accattggat agatgctgcc gccggcgttc tcggatgcgg
     4021 cgaaggcgca ttcggcgtcg cgaaaggcga tccaggcctt ctgcgccgcg acaagcagct
     4081 tggcggccgc ggcgtcgtcc ttgagacgcc gcatggtctc cctgtagacg ccgttcagcg
     4141 cgtcgtccgc cttcttgtaa gcggcgttgg cgcaggcgtt gagcccttgc tggttctggt
     4201 cgggacaatc ctcgccgaaa gcggggaggg cgagcgccag ccatgcgcca agcagaaagc
     4261 cgatccgtcg catcgccgag cccctccaat tgcgccggcg ccaatgctat tcacgcccgg
     4321 cgccgcggcg tgtcgccggc ggcgcgacaa attaaggctc ggcgcgcgca tgtccaagac
     4381 cattctcgtc accggcggct cgcgcggcat cggccgcgcc gtctgtcttc tcgccgccag
     4441 gcgcggctgg tcggtcggcg tcaattatgt ccacgacaag gaagcggcgg aggccatcgt
     4501 cgcgagcgcg gctcgcgaag gggcgagggc gctcgcaatt ccgggcgatg tcgccgagga
     4561 gagcgacgtc atcgccatgt tcgagaagac ggccgagacg ctcgggccgc tggacggcgt
     4621 cgttgtcaat gccggaatcg tcgcgccggc ggcgcggctc atcgagatga gcgtcgagcg
     4681 gatgcggcgc attttcgacg tcaacacgct cggcgcctat gtcacggccc gcgaggcggc
     4741 gcggcgcatg atgacgagcc agggcggcag gggcggatcg atcgtgctga tgtcctcggc
     4801 cgccgcgcgg ctcggctcgc ccgatctcta tgtcgactat gccggctcga aaggcgcgat
     4861 cgacaccctg acgatcggcc tcgcccagga gctcggccgc gaaggcgtgc gggtcaacgc
     4921 gatccggccc ggcatgatcg agaccgacat ccacgccagc ggcggcgagc ccggccgcgc
     4981 ccatcgcgtc ggcgcgaccg cgccgatggg ccggcccggc agggcggagg aaatcgccga
     5041 ggccgtcgtc tggctgctca gcgacgcggc gtcgtatacg accggcgcga tcctggacgt
     5101 ttccggcggg cggtgagggc ccgcgtcatt gcgagcgcgg cgaagctatc catcgtacgc
     5161 cgccgcgctg gaaacggatt gcttcgtcgc ttcgctcctc gcaatgacga aatttcaatg
     5221 aaacgccggc gaaatgaaga tcgcgatcgc gatcagcagc ccgacgaaga aggacgacga
     5281 gcgcagcggc tggcggtcgg tcaagtacat ggcggtgtag aagacgcgca caacgatgaa
     5341 ggcgaccgcc agccaattga cgagcgcgct cggcccgctg accacatggg cgatgatcac
     5401 ggcggcggcg aaaaagggaa acgcctcgaa atgattgaga tgggcgccat gagcgcgcgc
     5461 tggcaggcct tcgagatcgg cgacaagccg gcgcggcgtt ttgatgtcga gcttcgtcgc
     5521 ggctggccca aacaccacat aaggcaaaag accggcgatc agcacgcacc agaaagcgac
     5581 ggtcattggg ccccccacga tatttctcac ggcgagctta gcacattgaa gctgtgcgcc
     5641 gaagccgcgc gcgccgagct ttctcaagct ggcgacgccg cgagcgcccg gcgcaatgcg
     5701 caaattggcc tggcgtcctg atcggacgcc gaacgcccgc ttgctcgcgg cggctcgcca
     5761 ggctgccgca gcctgcctct atattattga aaataaagaa taaaaagcct atatagaatt
     5821 gcgccggact ggtggcgttg cggcgcaaaa tcgggtagaa aacacccgca ttttcgagat
     5881 aaggattcgc aggcttttga gcgattcaaa caacgagcgg ccggccggcg gcggcccctc
     5941 cgatattcgt cccgtttcga tcaccgacga aatgaagcgc agctatcttg attacgcgat
     6001 gagcgtgatc gtcagccgcg ccttgcccga cgcgcgcgac gggctgaagc cggtgcatcg
     6061 gcgcatcctg ttctcgatga gcgagcagaa tttcacgccg gaccggccgt acaacaagtc
     6121 gtcgcgcacg gtcggcgacg tgatcggccg gtaccatccg catggcgatc tcgccgtcta
     6181 cgaggccctg gtgcgcctgg cgcaggattt ctcgatgcgc gcgccgctga tcgacggcca
     6241 aggcaatttc ggctcggtcg acggcgatcc gccggcggcc cagcgctata ccgaagcgcg
     6301 gctcgaacgc gtggcgatgg cgctgctgga ggacctcgac aaggacacgg tcgatttccg
     6361 tccgaattac gacgaaaagg agttcgagcc ggtcgtcctg ccggcgcgct ttccgaacct
     6421 tctcgtcaac ggcgccggcg gcatcgcggt cggcatggcg acgaacattc cgccgcataa
     6481 tctcggcgag acgatcgacg cggttctcgc ggtgatggag cgaccggata tttcgctcga
     6541 cgatctcatc gaaatcattc cgggcccgga ttttccgacc ggcggcgcga ttctcgggcg
     6601 cggcggcatc cgcgcggcct acgccaccgg gcgcggctcc atcatcatgc gcgccaaggc
     6661 gcggatcgag gaaatccgca aggaccgcga agcgatcatc gtcgatgaaa tcccctacca
     6721 ggtgaacaag gcgtcgctgg tcgaacggat cgccgatctg gtgcgcgaga agaagatcga
     6781 aggcatcggc gatttgcgcg acgaatccga tcgcgacggc atgcgcatcg tgatcgagct
     6841 gaagcgcgac gcggtcgccg acgttgtgct caaccagctc tggcgcttca ccgccatgca
     6901 gacgagcttc ggctgcaaca tgatcgcgct gaacggcggc aagccggaat tgctgacgct
     6961 aaaggatatg ctgcaggcct tcgtcgactt ccgcgaggag gtcgttacgc ggcgcaccaa
     7021 gcacctgctc gcgaaagccc gcgacaacgc ccatctccag gtcggcctcg ccatcgccgt
     7081 cgccaatatc gacgaggtga tccgcctgat ccgcacctcg cccgacgccg ccgcggcgcg
     7141 cgaagccttg atggagcgga cgtggccggc gcgcgacatg gcgccgctcg tcgccctcat
     7201 cgccgatcca cgccatgccc tcgccgagga cggaacctgc cgcctctccg acgcccaggc
     7261 gcgcgccatt ctcgacttgc gcctccagcg tctcaccgcg ctcggccgcg acgagatcgc
     7321 cgccgccttg aacaagctcg ccgaagagat cgccgactat ctcgatatct taagctcgcg
     7381 agcgcgggtg cgcagcatca tccacgacga gatcgtcgcg gtgaaggagg ctttcgcgac
     7441 gccgcgtcgc accgagattc tcgacagcga ttccgacatg gaggacgagg acctcatcca
     7501 gcgcgaggat atggtcgtgc tggtcagcca tgccggctat atcaagcgcg tgccgctctc
     7561 gacctacaga gcgcaaaggc gcggcggcaa gggccgctcc ggcatgcaga cgcgggagga
     7621 ggatttcgtc gcccgcctgt tcatcgcctc gacccatcag ccggttttgt acttttcctc
     7681 taagggcaag gtttacaagg aaaaggtctg gcgcctgccg ttggcgccgc ctcaggcgcg
     7741 cggcaaggct ttggtcaatc tcctgccgct cgaagagggc gagcgcatca cgacgatcat
     7801 gccgctgccc gagaacgaaa aggattggga gaatctcgac gtgatgttcg cgacgacgcg
     7861 cggcaccgtg cgtcgcaaca agctctccga tttcgcccag gtcaatcgct ccggcaagat
     7921 cgcgatgaag ctcgacgaag gcgaggggat cgtcgacgtc cagacttgct cggaaaggga
     7981 cgacgtgctc ttgacgaccg cgcaaggcca atgcatccgc ttcgcggtgg atgacgtccg
     8041 ggtgttcaag gggcgcgatt cgatgggcgt gcgcggcatt gcgctcgccg agggcgacgg
     8101 cgtcatttcg ctggccatcc tgcgccaatt cgaggcgagc gccgaggagc gcgccgctta
     8161 tctcaaaatg cgccgcgcgg tcgccggcga ggtcggcgag aatggcaacg gcaatggcga
     8221 ggaggcgagc gacggcgacg ccgaggcggt cgccgaaacc gcgctgagcc aggagcgcta
     8281 tgcggaaatg tcggcggccg agcaggtcat cctcacgatc tcggagaatg gctacggcaa
     8341 gcgctcgtcc tccttcgaat atcgcgccac cggccgcggc ggcaaaggca tcgtcgccat
     8401 ggcggtcaac caacgcaacg gcaagctcgt cgcctccttc ccggtcgagc atgacgacga
     8461 aatcatgctg gtgaccaatg gcggccagct catccgctgt ccggtcgaag acatccgcat
     8521 cgccggccgc gccacccagg gcgtcatcgt cttcgacacc gccgacgcgg agcgcgtcgt
     8581 ctccgtcgag cgcatctccg agccggaaga agagcccgag gccgaagaga acggcggctg
     8641 aggacgcgcc cttctccccg cttcgcgggg cgaggggatc gcccgccgtc accccgtccc
     8701 cccgcaaagc gcggagaagg gaggactaca ccgcgcttcg cgcgtggacc acgtggccgc
     8761 cgggggttcc ggcgggcggc ggcgagggcg cgccgagata ggcggccatt tcgtgcggac
     8821 cgaaatcgtc gaaatgcgcg aagccggttt cgcgcagcag cgccgccata tccgctggcc
     8881 ggaaccggct gagcaaaggc tcgccgatcg ccgcgacgcg ggcttggaag gcctccatgg
     8941 cggcgcggcg cgcgccgctg aaggcgtcga gcggctcgcc gtagtcgaaa acgatttccg
     9001 ccgaggcgcg ggcgacggcg gtcaaggtgg agacgatggc gtcgcgcgtg aggtacggcg
     9061 tcacgccgag ccaaataaag aaggccggcg cttggcgatc gaatccggct ttggcgagct
     9121 cgtcgatgaa ggattgcttt tcgaaatcga cggcgacgaa gctcaacgcc ggcggctcgc
     9181 cggtcatcgc cgccgcgatc cgctcgcgtt tccaggcctg cgtcgccggg tggtcgactt
     9241 cgaaaacctt caagcctttg gcgcggtgcg gattgcgcaa gccgaaagtg tcgagcccgg
     9301 cgccgagcac gacaagctga cgcacgtggc gcgccacagc ctcggcaaga cgatcctcgg
     9361 cgagccggct gcgcgccgcg atgaacaggc gcatgaagcg ccgcgccgga tcgtcctcgg
     9421 cctcgcgacg aagcgcgtcc ggatcctcgc cgaggatcgc gaccgccagc ggatcggaaa
     9481 aaatccggcc gccttcgagc aattggtgaa ccgcgcgatg cgtcgcggcg cctttcgcgg
     9541 tgaggctggc ttgggcttgc atcgagagcg cggctcctca aatagcggcg acggtttgta
     9601 gccgcggtcg ccgaccgcgg ctccagcgct caccctttcg cgaatttccc cttcagcgcc
     9661 gccgcgacgt tcggcggcac gaaaggcgag acgtcgccgc ccatcgaggc gatctggcgc
     9721 accagcgtcg cggtgatgtg ccgcacattg cgcgatgcgg ggaggaagat cgtctgcagc
     9781 ttcggcgcca tgtcggcgtt catgccggcg agctgcattt cgtagtcgaa atcggtggcg
     9841 tcgcgcaggc cgcgcacgat cgccgaggcc cccgccgccg ccgccgcctc gaccgcgagg
     9901 ccggaaaaga cgatcgtctc cagcgcgccg ccgcccgagc gcaagagcgg cgcggcggcc
     9961 tcgccgatga gcctgacgcg ctcgtcggtc gagagcagcg cgaccttgct ggcatgcgcg
    10021 ccgacgccga cgacgagctt gtcgcaaagc ctcgccgccc gcccgatcac gtcaagatgg
    10081 ccgttggtga gaggatcgaa agacccggcg taaaagatga cgcgcgccat gccggcaaag
    10141 cgttagccgg cgccgatcgg gggcgcaagg gagcgcttca cccttttccg cccgccgcga
    10201 gcgccgccgc cacgtcctca atccgccggc tcgcatcgct gagcgcctcg gcgaccgcgg
    10261 cggcccattc gtcggcttcg cgccgcaccg cctcggcgac gtcgtcgcgc tcgcttccct
    10321 ttctttccag ttcgctgacc ttccttcgcg cctcgaacaa ttcatcggct atggtgagcg
    10381 cggccatgac gacgagtcgc tgatcgccga tttcgccgac ctggcctctg atttcggcga
    10441 ttttcgaatc gatctggcgc gccaggcctt caagatgcgg ctcctcgccc tcgttgcagg
    10501 cgaggcgata agatcgcccg ccgatcatca ccgtcacatg cgccatgcca gcgcctttcg
    10561 gaaattgcct ccgccgaggc ggccgatcgt cctcatcgcg cgacgagcca ggatatggcg
    10621 gacggatccc atgagaaagg ccctcccgct cactccggct ccgggacgcg atggcgttcg
    10681 aggatcgagc ggattgcggc gctcgccttc tcgacacgcc gcgccgcctc catattggcc
    10741 gcgagcaggc cgcgatttcg gtcgagcgcg ccgtcaagct ccacggcgag gcgggcgcgg
    10801 tcgtcctgca tcaaagccag ctcttcctgc gtgtcgccgc gcgcgccttc ctgccgggct
    10861 cggcgctcga tcgcggcttc gagcaggtcg agcgcggcgc gcagccgctt gaaggaaggc
    10921 tcgagaagat cggcgctcat taacttaccg aagcttgcat tccgtcgttt acgcttcggt
    10981 tgaagcgagc gtcaacgcgg cggaacgctc gcccacaggg gaatttgcaa gaccggccgg
    11041 caaaccgatc gcggccggcg gcggaaactt tgccgaacgg acaggcattt gacaggccaa
    11101 gcccaaggcg cctatcaacc gccgcctcgc gcttaaagcg ccgacgttca gaaaacccac
    11161 gattcgggag agcagcatgg cggcgaatgt gacgcatgac cagatggcca acgccattcg
    11221 cgcgctttcg atggacgcgg tcgaaaaggc caatagcggc catccaggca tgccgatggg
    11281 catggccgac gtcgcgaccg ttttgtttcg cgacgtcttg aaattcgacg ccgccgatcc
    11341 ggcctggccc gaccgcgacc gcttcgtcct gtcggcgggc catgggtcga tgctccttta
    11401 ttccctgttc tacctgaccg ggacaggcgg catggcgatc gaggatttga aacagttccg
    11461 ccaggtcggc tcgaaaacgc cgggccatcc cgaatatcgc cacacgatcg gcgttgagac
    11521 gacgaccggg cccctggggc agggtctcgc gacctccatc ggcatggctc tggccgaaac
    11581 catgctcgcc gcagagttcg gcgatctcgt cgaccatcac accttcgtca tcgcctcgga
    11641 cggcgatctg gaggagggcg tcagccacga agcgatctcc atcgccggcc atttgaggct
    11701 caacaagctc atcgtccttt gggacaacaa ttcgatctcg atcgacggca agacctcgct
    11761 cgccgattcg gtcgatcatg tggcgcgatt caaggcggcg ggctggcgcg ccgcccatat
    11821 cgacggccat gacccgaagc aggttctgcg cgcgttgcgc gccgccaaaa ggtcggaccg
    11881 gcccaccatg atcgcctgca aaacgacgat cggcttcggc ttgccgacca aggccggcac
    11941 caacaaggcg catggcgagg cgcccggcgc cgccgaggtc gccggcgcgc gcgtcaatct
    12001 gcattggccc tatgagccct tcgtcgtgcc ggacgacatt ctggaggcgt ggcgggcggt
    12061 cggccggcgc ggcaaaagca agcgcaacag atggcggcgc ctgctcaaga gcaagccgcc
    12121 ggaaatccgg gcggaattcg agcgccgcct ggccggcgaa ttgccgaagg gtctcgatga
    12181 ggtcatcgac gcctacaagc ggaagctcgc cgccgacgcg ccggaaatcg ctacccgcaa
    12241 ggccggcgaa gccgcgctga aagtcatcgc gccggccgcg ccggaactca tcaccggctc
    12301 ggccgacttg accggctcga acaacaccaa ggtcgaggcg acgcgggaga tcacgccgga
    12361 ggacaaatcg ggccgctaca tccattgggg cattcgcgag cacggcatga tcgcggcctg
    12421 caacggcctc gcgctgcatg gcggcttcat cccgtccggc gcgagcttcc tcgtcttcac
    12481 cgattattgc cggccctcgc tgcgccttgc ggcgctgatg ggaatccgcg tctgccacgt
    12541 cttcacccac gattcgatcg gcctcggcga agacgggccg acccaccagc cggtcgagca
    12601 tctggcgtcc ttgcgcgcca tgcccaacat gttcaccttc cgcccggccg attccgtcga
    12661 gacggtcgag tgttggcagg cggcgctgaa gatgaggcgg tcgccgtcga tcctggcgct
    12721 gacccggcag aacctgccgg cgctgcgccg cacgcatgtc gcggaaaact taagcgcgcg
    12781 cggggcctac gaaatctcgc cggccgcggg caaagcggcg gtgtcgattt tcgcctcggg
    12841 ctcggaagtg tcgctcgccg tggcggcgca aaaactcctc gctgagaagg gcgtcgtcgc
    12901 ccgcgtcgtc tctgtgcctt gcatggacct tttctttgag cagaaggagg cgtatcgcca
    12961 gtcggtgatc ggcgacgcgc cggtgaaagt cggggtcgag gccgcggtcc gccaagggtg
    13021 ggacgcgatc atcggcgacg ggccgttcat cggcatgaag ggcttcggcg agagcggtcc
    13081 ctataaggcc gtttacgagc atttcggcat cacgccgcaa gcggtcgccg aagcggcgtt
    13141 ggcgaggctg tgatcttgga aattggagcc gcggtcggcg accgcggctc cggcgcggcg
    13201 gcgcaaaaag agcgagacgc gcgccgcgtt ttgcggcaaa ggagagccgg ctgagcggaa
    13261 ccccgcaagg cccgaagcgg taattggggt cgcaaggaga ttttcgatgg cggtgagagt
    13321 ggcgatcaac ggctttggac ggatcgggcg caacgtcttg cgcgccattt tcgagtccgg
    13381 gcgcaaagac atcgaggtcg tcgcgatcaa cgatctcggt ccggtcgaga ccaacgccca
    13441 tctcttgcgc ttcgacagcg tccatggccg ctttccgcac gaagtgaagg tcgagggcga
    13501 cacgatcaat atcggcgtcg gcaagatcaa agtgaccgcg atcaaggatc cggcccagct
    13561 tccgcacaag gagctcggcg tcgagatcgc gctcgaatgc accggcattt tcaccgcccg
    13621 cgacaaggcg gcggcgcacc tgaccgccgg cgccaagcgg gtgatcgtct cggcgccatc
    13681 ggacggcgcg gacttgacgg tcgtttatgg cgtcaatcac gaaaagctga ccaaggagca
    13741 cttggttatt tcgaacgcct cgtgcacgac caattgcctg gccccggtcg ccaaagtgct
    13801 caacgacacg atcggcatcg accacggctt catgacgacg atccattcct atactggcga
    13861 ccagccgacg ctcgatacgc tgcacaagga cctctatcgc ggccgcgccg cggctttgtc
    13921 gatgattccg acgacgaccg gcgccgccaa ggcggtcggc ctggttcttc cggagctcaa
    13981 gggcaagctc gacggctcgg cgatccgcgt gccgacgccg aacgtctcgg tggtcgattt
    14041 gaaattcgtc gccaagcggc cgacgagcaa ggacgagatc agtacggcga tcaagaacgc
    14101 cgcggcgcag caattgaaag gcattctcgg ctacaccgat cagcccaatg tctcggccga
    14161 tttcaatcac gacagccgtt cctcggtctt ccatctcgat cagaccaagg tgatcgacgg
    14221 gacgttcgtg cgcgttctgt cctggtacga caatgaatgg ggcttctcga atcgcatggc
    14281 cgacaccgcc gtggcgatga gcaaggtgat ctgagccgct ttccacgctc gatcgggccg
    14341 cttgcggcgc ggaagcgcga gcggccgcct cgccttcctt ccgcgaggcc tcgtcgatga
    14401 gctcttcctt ccgcacgctg gacgacgctt ctcttgcggg aaagcgcgtc ctccttcgcg
    14461 tcgatctcaa cgtcccgatg gagcatggcc gcgtcaccga cgcgacgcgc atcgagcgcg
    14521 tgctgccgac catccatgag atttcgcgaa aaaggggcaa ggtcattctg ctggcgcatt
    14581 tcggccggcc gaaaggcggc ccagacgacg ccaattcgct gcggccggtc gccgccgcgg
    14641 tcgaggcgtt gctgaaacag ccggtcgcct tcgcgcccga ttgcatcggc gaggccgcga
    14701 cgaaggcgat cgcggcgatg aaggacggcg atgtccttct gctggagaac acgcgctttc
    14761 ataaaggcga ggaaaagaac gatccggctt tcgtcgccga gctcgccaag ctcggcgagc
    14821 tctatgtcaa cgacgccttt tccgccgccc atcgcgccca tgcctcgacc gagggcctgg
    14881 cgcatcgctt gccggcctat gcggggcgcg ccatgcaggc cgagctcgaa gcgctgggca
    14941 aggcgctgga ggcgccggcg cggccgctgg tcgcgatcgt cggcggggcg aaggtttcga
    15001 ccaagctcga tctcctcggc aatctcgtca cgaaggtcga cgctttggtg atcggcggcg
    15061 gcatggcgaa cacgtttttg cacgcccagg gctatcccgt cggcaagtcg ctttgcgaga
    15121 aggacctcgc cgacaccgcg cgggcgatca tggccaaggc gcaggagagc cgctgcgcga
    15181 tcatcctgcc gctcgacgcg atcgtcgcct ttcatttcgc cgccaacgcg ccggcccatg
    15241 attatggaat cgacgccatc ccgcccgagg gcatgatgct cgatatcggc cagctctcgg
    15301 tcgaccggat caaggcctcg atcaacgaag ccgggacatt ggtctggaac ggcccggtcg
    15361 gcgccttcga gctcgctggc ttcgagcacg gcacgatggc gctcgcccgc cacgtcgcgg
    15421 agcgcaccaa agcgcacaag ctcgtctcgg tggcgggcgg cggcgacacg gtcgccgcgc
    15481 tcaacgccgc cggcgtcgtc gatcaattca cctatgtgtc gaccgccggc ggcgcctttc
    15541 tcgaatggat ggaaggcaaa gcgctgcctg gcgtcgaggc gctgcgacgg ccggcgtgaa
    15601 ggtctgaagg cgtac
//
LOCUS       DATGJW010000313         5017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1097987, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000313 DATGJW010000000
VERSION     DATGJW010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5017
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1097987"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1007)
                     /locus_tag="VKU03_09570"
     CDS             complement(<1..1007)
                     /locus_tag="VKU03_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HLJ71555.1"
                     /translation="MLAFLFAPLAAPAAEKIVPQSPVEVHLSFAPVVKKVRPAVVNVY
                     ASRIETRPRNPLLDDPIFRHFFGEQGGTGRTAQSLGSGVIVDRSGLVVTNNHVIEGMN
                     EVKVALADHREYEAKILLRDPRSDLAVLKIKDGGDNFPVLELGDSDAIEVGDFVLAIG
                     NPFGVGQTVTQGIVSALARTQIGANDYGFFIQTDAAINPGNSGGALVDLDGKLIGINS
                     EIISRSGGSMGLGFAIPVNMVKSVIAAARGGDRLVRRPWLGASLQNVTQDIAESLGLD
                     KPVGALVAGVVDGGPAAEAGLKRGDIIVAVDGVEVDDSGGVGFRLGVKPLGGEARLGI
                     IR"
     gene            complement(1233..2120)
                     /locus_tag="VKU03_09575"
     CDS             complement(1233..2120)
                     /locus_tag="VKU03_09575"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71556.1"
                     /translation="MEVGVIGLGAMGRGLAVRLIKAGHRVKVWNRSPGPAEALAAEGA
                     TRCASAAEAASGEALVSMLANDAVYREIFLANGALDATRKGAVHINMATISVALGKEF
                     AERHRQRGVLYVAAPVLGRPDAAAAGQLNILAAGAPAAIDKARPLLDAMGQRVWPFGE
                     LAEQANAAKIAANFMIASAVEAMGEAMTLLDAYGVGADDFLEAMTGSLFAAPIYKSYG
                     KRIVEGQYDPPGFKLALGFKDIRLALEASEAKAVPMPLASLLRDGHLNSMAHGEGDLD
                     WAALAKVAFRRAKRRPPGG"
     gene            complement(2245..2634)
                     /locus_tag="VKU03_09580"
     CDS             complement(2245..2634)
                     /locus_tag="VKU03_09580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4260 domain-containing protein"
                     /protein_id="HLJ71557.1"
                     /translation="MSAAALGAVQGGPRLILRLEGAALAIGAAFFYWRLGGSWLLFVL
                     LFLAPDLSFLAYLLSPRAGAVGYNALHATIGPLVLLVIGAWTDRSPCEWVALIWLAHI
                     GFDRALGYGLKYAEAFNTTHLGRIGRN"
     gene            complement(2631..3056)
                     /gene="rplQ"
                     /locus_tag="VKU03_09585"
     CDS             complement(2631..3056)
                     /gene="rplQ"
                     /locus_tag="VKU03_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HLJ71558.1"
                     /translation="MYHGRAKRRFNRTAEHRVAMFANMAQALIKHEQIVTTLPKAKDL
                     RPVVEKLVTLGKRGDLHARRLAISRLRDADLVKKLFDVLGPRYKTRNGGYTRVLKAGF
                     RYGDNAPLAVIEFVDRDPEAKGKDSGPAQEKKAEAEAAA"
     gene            complement(3177..4193)
                     /locus_tag="VKU03_09590"
     CDS             complement(3177..4193)
                     /locus_tag="VKU03_09590"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HLJ71559.1"
                     /translation="MIQKNWQELIKPKKLEVAQSDDPKRVATVVAEPLERGFGHTLGN
                     ALRRVLLSSLQGAAITSIHIDGVLHEFSSIPGVREDVTDIVLNVKDIAIKMQGEGPKR
                     LVLKRHGPGVVTAGEIQTSGDIQILNPNLPICTLDEAAEIRIEFTVTTGKGYVPAERN
                     RAEDSPIGLMPIDSLFSPVKKVSYRVENTREGQVLDYDKLIMTIETNGAITPEDALAY
                     GARILQDQLSVFVNFEEPRREEAAQAIPELAFNPALLKKVDELELSVRSANCLKNDNI
                     TYIGDLIQKSEAEMLRTPNFGRKSLNEIKEVLAQMGLHLGMEVTGWPPENIEDLAKRF
                     EEHY"
     gene            complement(4310..4708)
                     /gene="rpsK"
                     /locus_tag="VKU03_09595"
     CDS             complement(4310..4708)
                     /gene="rpsK"
                     /locus_tag="VKU03_09595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HLJ71560.1"
                     /translation="MARGTAAAPRLRRRERKNITSGTAHVNATFNNTMITITDAQGNT
                     ISWSSAGTMGFKGSRKSTPFAAQMAAEDAVRKAAEHGMRMLEVEVSGPGSGRESALRA
                     IQAAGFNVTSIRDVTPIPHNGCRPRKRRRV"
     gene            complement(4748..>5017)
                     /gene="rpsM"
                     /locus_tag="VKU03_09600"
     CDS             complement(4748..>5017)
                     /gene="rpsM"
                     /locus_tag="VKU03_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HLJ71561.1"
                     /translation="CNKVGIPLDRRANDMTDDEVLRVRELIDREYTVEGDLRRQVAMN
                     VKRLMDLGCYRGLRHRKGLPVRGQRTHTNARTRKGPARPIAGKKK"
BASE COUNT          850 a         1623 c         1651 g          893 t
ORIGIN      
        1 cggatgattc caagcctggc ttcgccgcca agcggcttga cgccgagccg aaagccgacg
       61 ccgccggaat cgtcgacctc gaccccgtcg accgcgacga tgatgtcgcc gcgcttcagg
      121 ccggcctccg ccgccggccc gccgtcgacg acgcccgcga cgagcgcgcc gaccggcttg
      181 tcgaggccca gcgattcggc gatgtcctgc gtgacgttct gcaggctcgc gccgagccat
      241 ggccgccgga ccaggcgatc gccgccgcgc gccgcagcga tcacgctctt gaccatattg
      301 accgggatgg cgaagccgag gcccatgctg ccgccgctgc gcgagatgat ctcggaattg
      361 atgccgatca gcttgccgtc gagatcgacc agcgcgccgc cggaattgcc gggattgatc
      421 gcggcgtcgg tctgaatgaa gaagccgtaa tcgttggcgc cgatctgggt tcgggcgagc
      481 gccgaaacga tgccctgcgt gacggtctgg ccgacgccga acggattgcc gatggcgaga
      541 acgaaatcgc caacctcgat cgcgtccgaa tcgccgagct ccagcaccgg aaaattgtcg
      601 ccgccgtcct tgatcttcaa cacggcgaga tcgctgcgcg gatcgcgcaa aaggattttg
      661 gcctcgtatt cgcgatggtc ggccagggcg accttcactt cgttcatgcc ttcgatgacg
      721 tgattgttgg tgacgacgag gccggaccgg tcgacgatga cgccggagcc gagcgattgc
      781 gcggtgcgcc cggttccgcc ctgctcgccg aagaaatggc ggaagatcgg atcgtccaaa
      841 agcggattgc gcggccgcgt ctctatccgc gacgcgtaga cgttgaccac cgccggccgg
      901 accttcttga ccaccggcgc aaaggagaga tgaacctcga cgggggattg cgggacaatt
      961 ttctccgctg ccggcgccgc aagcggcgcg aagaggaaag cgagcaggaa gcaggcgaca
     1021 aggcgagaca agatcgtacc tttcagtttt gtgcagctcc cttctccccg ccttgcgggg
     1081 agaaggtggc gcgaagcgcc ggatgagggg ctgcgccgga ctcgaaagta cacgcccttc
     1141 gaacgctaac ccgcaagctc agcgccgccc ctcatccggc cgtgcttcgc acggccacct
     1201 tctccccgct tcgcggggcg aagggaaaat cctcacccgc ccggagggcg gcgcttggcg
     1261 cggcgaaacg cgactttggc gagcgcggcc caatcgagat cgccttcgcc atgcgccatg
     1321 gaattgaggt ggccgtcgcg cagcaggctg gcgagcggca tcggcacggc cttggcctcg
     1381 ctcgcctcaa gggccaggcg gatgtccttg aagccgagcg ccagcttgaa accgggcgga
     1441 tcatattggc cttcgacgat ccgcttgccg tagcttttat agatcggcgc ggcgaagagg
     1501 gagccggtca tcgcctcgag aaaatcgtcc gcgccgacgc cataagcgtc gagcaaagtc
     1561 atcgcctcgc ccatcgcctc gaccgcgctg gcgatcatga aattggcggc gatcttggcg
     1621 gcattggctt gctcggcgag ctcgccgaac ggccagacgc gctggcccat ggcgtcgagc
     1681 agcgggcgcg ccttgtcgat cgccgccggg gcgcccgccg ccaagatatt caattgtccg
     1741 gccgccgccg cgtcgggacg accgagcacg ggcgccgcga catagaggac gccgcgctgg
     1801 cgatggcgct cggcgaactc cttgccaagc gcgaccgaga tcgtcgccat attgatgtga
     1861 accgcgcctt tccgcgtcgc gtcgagggcg ccgttagcga ggaatatttc gcgataaacg
     1921 gcgtcgttgg ccagcatgga aaccagcgcc tcgccgctcg cggcttcggc ggcgctggcg
     1981 cagcgcgtcg cgccttcggc agccagcgcc tcggccggac cgggcgagcg gttccagacc
     2041 ttgacgcgat ggcccgcctt gatcaggcgc acggcgaggc ccctgcccat cgcgccgaga
     2101 ccgatcactc cgacttccat ggcgttctcc aaatttgcga ggtagccgtc attgcgcgga
     2161 gcgaagcgac gaggcgatcc aggagccgcg gcggttcctg gattgcttcg cttggctcgc
     2221 aatgacgggg ttggttgtca tcagtcagtt ccgcccgatc cgccccagat gcgttgtgtt
     2281 gaacgcttcc gcgtatttga ggccgtagcc gagcgcccga tcaaagccga tatgagcgag
     2341 ccagatcaac gcgacccatt cgcacgggct ccggtccgtc caagcgccaa tgacgagaag
     2401 gaccaacggc ccgatcgtcg catgcagcgc gttgtacccg accgcgccag cccgcggcga
     2461 gaggagatag gccagaaagg acaagtccgg agcaaggaag aggaggacga agagcaacca
     2521 cgagccgccg agtcgccaat agaaaaaggc ggcgccgatc gccagcgccg ccccttccaa
     2581 ccgcaagatg agccgcgggc cgccttgaac ggcgcccaag gccgcggcgc tcacgccgcc
     2641 gcctcagcct cggccttctt ttcctgagcc ggtccggaat ccttgccctt ggcctccggg
     2701 tcgcgatcga cgaattcgat caccgcgagc ggcgcgttgt cgccatagcg gaagccggcc
     2761 ttgagcacgc gggtatagcc gccattgcgc gttttgtagc gcggcccgag cacgtcgaag
     2821 agctttttga ccaggtcggc gtcgcggagc cgcgagatgg cgagccggcg cgcatgaaga
     2881 tcgccgcgct tgccgagcgt gaccagcttc tcgaccaccg gacgaagatc cttcgccttg
     2941 ggcaaggtgg tgacgatctg ctcgtgcttg ataagcgctt gcgccatatt ggcgaacatc
     3001 gcgacgcgat gctcggccgt gcggttgaag cggcgcttgg cgcgtccgtg atacatgtcg
     3061 gctctccttt atgcggccgc cgtgccaagg aacggccggg tgttctcggc agtcggcaga
     3121 tcggcagtcg gcaatcgaga acccgactgc cgattgccga cctgccgact gccgtctcag
     3181 taatgctcct cgaaccgctt ggccaggtcc tcgatattct ccggtggcca accggtgact
     3241 tccatgccga gatgcaggcc catttgggcg agcacttcct tgatctcgtt gagcgacttg
     3301 cgaccgaaat tcggcgtgcg cagcatctcg gcttccgact tctggatcag gtcgccgata
     3361 taagtgatgt tgtcgttctt caggcaattg gccgagcgca ccgacaattc gagctcgtcg
     3421 accttcttga gcaaagccgg attgaaggcg agttcgggga tggcctgggc ggcctcctcg
     3481 cggcgcggct cttcgaaatt gacgaagacc gagagctgat cctgcaggat gcgcgcgcca
     3541 taggcgagcg cgtcttccgg cgtgatcgcg ccgttggtct cgatcgtcat gatcagcttg
     3601 tcgtaatcga gcacctggcc ctcgcgggtg ttctcgacgc gataggagac tttcttaacc
     3661 ggcgagaaca ggctgtcgat cggcatcaga ccgatcggcg aatcctcggc gcggttgcgc
     3721 tcggccggca catagccctt gccggtcgtc acggtgaatt cgatgcggat ttcggccgcc
     3781 tcgtccagcg tgcagatcgg caaattgggg ttgaggatct ggatgtcgcc tgaggtctgg
     3841 atttcgccgg cggtgacgac gcccgggcca tggcgcttga gcacgaggcg cttcgggccc
     3901 tcgccctgca tcttgatcgc gatatccttc acgttgagga cgatatcggt cacgtcctcg
     3961 cgcacgccgg ggatcgacga gaattcatgc agcaccccgt cgatatggat cgaggtgatc
     4021 gcggcgccct gcagcgaaga caaaaggacg cggcgcagcg cattgccgag cgtatggcca
     4081 aagccgcgct cgagcggctc ggcgacgacg gtcgcgacgc gcttgggatc gtcgctctgg
     4141 gcgacttcga gtttcttcgg cttgatcagc tcttgccaat tcttctggat cacgggcggg
     4201 gccttccgtt cgacgcgctc gaggcgagag ccgcctggcg catgccgcaa ctcaacttgc
     4261 cgaagcgcgc tgcggccggc gcgcttccct gtctcattcc atcgagcgct tagacgcggc
     4321 ggcgcttgcg cggccggcag ccattatggg gaatcggcgt cacgtcgcgg atcgaggtga
     4381 cgttgaaccc agcggcctgg atggcgcgca aagccgattc gcggcccgaa ccggggccgg
     4441 aaacctcgac ttcgagcatg cgcatgccgt gctcggccgc tttgcggacc gcgtcctcgg
     4501 ctgccatctg ggcggcgaag ggcgtcgatt tgcgggagcc cttaaagccc atcgtgccgg
     4561 ccgacgacca ggaaatcgtg ttgccctgcg catcggtgat ggtgatcatc gtgttgttga
     4621 acgtcgcgtt cacatgggcg gtgcccgagg tgatgttctt gcgttcgcga cgccgcagcc
     4681 gcggcgctgc tgccgtacct cttgccattt agccgttccg ccgtgtcgag gggataatca
     4741 aaagaaccta tttcttcttg ccggcgatcg gccgggccgg gcctttgcgc gtgcgcgcat
     4801 tggtatgcgt gcgctggccg cgcaccggca ggcccttgcg gtgccgcagg ccgcggtagc
     4861 agccgaggtc catcagccgc ttgacgttca tcgccacctg gcgacgcaaa tcgccctcga
     4921 cggtgtattc gcggtcgata agttcgcgga cacgcaacac ttcgtcgtcg gtcatgtcgt
     4981 tggcccggcg atcgagcggg atgccgacct tgttgca
//
LOCUS       DATGJW010000314         9607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_681866, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000314 DATGJW010000000
VERSION     DATGJW010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9607
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_681866"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..193
                     /locus_tag="VKU03_09605"
     CDS             <1..193
                     /locus_tag="VKU03_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF3095 family protein"
                     /protein_id="HLJ71562.1"
                     /translation="LERRLQQASDFAEYGAFRQSNALLTCYAPSLTDRSHIHFVDGAG
                     GGYAMAAKAMKERRLRAAA"
     gene            complement(256..1035)
                     /locus_tag="VKU03_09610"
     CDS             complement(256..1035)
                     /locus_tag="VKU03_09610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654233.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HLJ71563.1"
                     /translation="MSAPMIEIKRVSKSFGPLQVLREVDLAVGRSEVVCLIGPSGSGK
                     STLLRCVNFLETYDSGDIRIDGRLIGYRDAAGGRQPMPARELREMRREIGMVFQHFNL
                     WPHMSALQNVAEPLKRVRGLKPAEADERAEAMLKRVGLADKAGAYPGRLSGGQQQRVA
                     IARALAMQPRLMLFDEPTSALDPELVGEVLEVMRDLAAEGMTMLIVTHEMGFAAHVAD
                     RIAFIDSGRIIDQGPPKKVFHESDEPRVRQFLQTYHDRNSF"
     gene            complement(1032..1700)
                     /locus_tag="VKU03_09615"
     CDS             complement(1032..1700)
                     /locus_tag="VKU03_09615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HLJ71564.1"
                     /translation="MSLQVFLHYLPSLLSGFGVTVLCWSAGSIIGLAVGFVVTLLNRL
                     RFPPLHWLLRVYVEILRGTPFLMQLFLLYAGGPFIGLRLSATAAGIVGLGLYSSAYFA
                     EIFRAGFAAVPQGQIEAAMSVGMRSWDILFRIRAPIALTATIPSIVNMLVILSKETVV
                     LSIITVPELMYQMQTMAAETFTAFDAIFAMAVFYWILVEAVSRAGRRLEIKVTAFMAR
                     EAAA"
     gene            complement(1697..2353)
                     /locus_tag="VKU03_09620"
     CDS             complement(1697..2353)
                     /locus_tag="VKU03_09620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007697992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HLJ71565.1"
                     /translation="MRFDIILHSLGYLLQAAGVTIALSLAGIALGFAIGALVCAARLS
                     ANAALRRLGGFYVSFFRGVPLLVQLLFIYYFLAEYGLDVPAVVAAIGGLGFASGAYQA
                     EILRGALNVVPRGQAEAATALGFAPSDIWRRILLPQALRISAPPLINEFILLLKASSL
                     VSVVGIAELTRVSMNIASMTYRPFEAYLSGGLVYLAINLCLAAFGVFAERRLAGGRPT
                     "
     gene            complement(2537..3391)
                     /locus_tag="VKU03_09625"
     CDS             complement(2537..3391)
                     /locus_tag="VKU03_09625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLJ71566.1"
                     /translation="MTIFDTRGLSRRGLLKNGLFGAAASGAFLGGFGRAFADTLADVK
                     SRGELVAATEMQFPPFDISDNGVYKGVDRELVDAVGATLGVKVKYLDLPWTSILPGLD
                     AKKFDICIAPVTITKERMKRYLFTVPIADATAALMKRANDNSINKPEDIAGKTVGGQK
                     GTSQLAQLKEFAAKLPKPVEVKEYVDNNQSYADLAAGRIDASVNSLPNLAYAANQRSD
                     TFKVVLPPFGHPTYFSWVGRLEDKTFIDAINAAILKAEADGTMAKIQKKWFGQSGDLP
                     KTVPEPTI"
     gene            complement(3617..4510)
                     /locus_tag="VKU03_09630"
     CDS             complement(3617..4510)
                     /locus_tag="VKU03_09630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLJ71567.1"
                     /translation="MSEQLTLFAPASLTRTRRRSRGQKPFDLYPLFRLAVLLLVSSVV
                     LLPLLATAIGGFKELGELRTNPLGLPHPWRWRNYLDILESARYWRVLGNSLFIACFTV
                     AITVALSAMAAFAFAHLKFFGSRFLFTYMTLGLMFPIATAILPLFIRVRDLGLLDTPW
                     GVILPQSAFYLAMAVLLLRNGFRQLPSELLDAALVDGCGYIRYFLYVTLPLSRPILST
                     VAIIAFVNSWNNYIVPLIMLNSESLYPWPLGMMAYEGEYMTSWQLVLAFITLTILPAI
                     IMFLLAQKHIVAGLTAGAVKG"
     gene            complement(4503..5399)
                     /locus_tag="VKU03_09635"
     CDS             complement(4503..5399)
                     /locus_tag="VKU03_09635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012067160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLJ71568.1"
                     /translation="MTVTTVQESKSAAGGVWVAALIFLPPALLLFTVFVILPIGEAAW
                     YGFYNWDGYNRPQNFIGLKNYISLFNNSNFRTAALNNLIIIAVSLAIQLPLALAMALL
                     LAKRAFGAVAFRLIFFLPYILADVAAGLIFRFIFDGDYGPLSGASQALGFGPVYMLAS
                     PTWAMVALLVVIVWKYFGFHMMLFIAGLQGIDRNLYEAAEIDGAGAFQRFRFVTIPQL
                     SPMIKLSVFFAVIGALQFFDVVVPLTGGGPFNATNTMVSFLYLFGITRMRVGFGSAVG
                     VVLFIICVSFAFGYRRLLMRDE"
     gene            complement(5645..6904)
                     /locus_tag="VKU03_09640"
     CDS             complement(5645..6904)
                     /locus_tag="VKU03_09640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182802.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLJ71569.1"
                     /translation="MKFRTLALASFCLAFGASAACADTTIRWLHLSNDDAGTMALYNQ
                     IIHDFEAQNKGVKIQLQDLENEAFKAKLPTMLQSDKDRPHIFYSWGGGVMRAQAKAGF
                     LEDITAAAAPWANTISPTAVEAFQVDGKTYGIPQNTGEVVFFYNKKLFAKAGVKAEDI
                     KTWDDFLGAVKKIKAAGITPIIVGGGEKWPMHFYWSYLVIRGGGANALADAKAGKDGG
                     FKNATFVEAGKRLHELAALEPFQAGYLSTSHPQAEGMFGDGKGAMELMGQWLLTAQGP
                     NAADGKGQPIDNIGIFSFPTLPGGKGQPTDTLGGINGWLVGKGAPKEAAEFLKFFSQE
                     KYQREAAAKGLYIPVVKGAESAIENPVLKQIGADLAKTTYHQNFFDQDLGPSVGRVVN
                     DISVAVAAGKMSPEDGAAAVQQAWDEQ"
     gene            7113..8324
                     /locus_tag="VKU03_09645"
     CDS             7113..8324
                     /locus_tag="VKU03_09645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HLJ71570.1"
                     /translation="MKTADPELMRAINRFHVMDSIRRHGPISRVEICGRTDLSPTTVS
                     AITAALIEDGLIVARPIGDVRDATRGRPRVMLELNPEAAYVVGVKLAPDQITIAATNF
                     RADVLASLALPIRISRQPASVVADLVEDGVRRCLADADLTVARIAGLCVGLPGVVERA
                     SGVCRQSTVFGERDLPFAKELSGRLGLPVSIDTDVNLVTLAEHWFGEVKGLSDFLVVS
                     IEDNLGLGIMHNGELFRGAGGLSPDLGDLLVRPNAGDGRNRLASIASETAIVGEALDA
                     VDGKRDRFAKIGKELDKVIELAQEGDQRFIGILAAAGDALGFAIANLITLFAPPKVIL
                     GGAAVKAGPLLLEPLRETASTLVPASLAHVAEIVVHQWSDEMWARGAAAMTLRDLYGA
                     PWNTTGPALLR"
     gene            8350..9447
                     /locus_tag="VKU03_09650"
     CDS             8350..9447
                     /locus_tag="VKU03_09650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182800.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ71571.1"
                     /translation="MKRVGIGVIGCGYISAAYLKAARLFPILEVRALADLNREAAEAR
                     AKEFGLKAVSIEKLLSDPKIEIVLNLTIPKAHVAVGLQAIEAGKHVHSEKPLGIAVAE
                     AKKLIKAAKIRGLRIGCAPDTFLGGAHQTCRKLIDNGRIGTPVGGTAFFMCPGHERWH
                     PNPDFYYEKGAGPMLDMGPYYITALVDLLGPVARVAGVATKLRKERVISSEPRRGETI
                     AVSTPTHIAGTLEFASGAAISIVMSFDVPKHGHKPLEIYGTKGSLIVPDPNFFDGQIE
                     IAAAGEDWGPVATEHAYADGNYRIIGAADMAQAIRAGRPHRANGDLAFHVLEVMEAFG
                     RSSKSGAFVEIKSRPERPAPLPVGLKTGELD"
BASE COUNT         1861 a         3013 c         3110 g         1623 t
ORIGIN      
        1 gttggagaga cgcctgcaac aggcgagcga cttcgccgaa tacggcgcct ttcggcaaag
       61 caacgcgctt ctgacctgct acgcgccctc gctgaccgac cgcagccata tccatttcgt
      121 cgacggcgcc ggcggcggct acgccatggc cgcgaaggcg atgaaggagc ggcggctgcg
      181 cgccgcggcg tgacgccccg cactgcgcga atcctttcgc cccgcgaagc ggggacaagg
      241 ggttcccgct gctagctaaa aactgttcct atcgtggtac gtctgcagga actgccgcac
      301 ccgcggctcg tcgctttcgt ggaagacctt tttcggcggg ccttggtcga tgatcctgcc
      361 gctatcgatg aaggcgatgc ggtcggcgac gtgggcggcg aagcccattt cgtgggtgac
      421 gatcagcatc gtcatgccct cggcggcaag atcgcgcatc acttcgagca cctcgccgac
      481 aagctccgga tcgagggcgg aagtcggctc gtcgaacagc atcagcctcg gctgcatcgc
      541 cagcgcccgc gcgatggcga cgcgctgctg ctggccgccg gagagccggc cgggataggc
      601 gccggccttg tcggcgaggc cgacgcgctt cagcatcgcc tcggcccgtt catccgcttc
      661 ggccggcttg agcccgcgca cccgcttcaa cggctcggcg acgttttgca gcgcgctcat
      721 atgcggccag agattgaaat gctggaacac catgccgatc tcgcgccgca tctcgcgcaa
      781 ttcgcgcgcc ggcatcggct ggcggccgcc ggcggcgtcg cgatagccga tcagccggcc
      841 gtcaatgcgg atatcgccgc tatcgtaggt ctcgagaaag ttgacgcagc gcaggagcgt
      901 gcttttgccc gagccgctcg ggccgatcag gcagaccacc tcggagcggc cgaccgcaag
      961 atcgacctcg cgcaagactt gcagcggacc gaagctcttc gaaacgcgct tgatctcgat
     1021 catcggcgcg ctcatgccgc cgcctcgcgc gccatgaagg ctgtgacctt gatctccaag
     1081 cgccgtccgg cccgcgagac cgcctcgacc aggatccagt agaagaccgc catggcgaaa
     1141 atcgcgtcga aagccgtgaa ggtctcggcg gccattgtct gcatctggta catcagctcg
     1201 ggcacggtga tgatcgacaa gaccacggtc tctttcgaga ggatgaccag catattgacg
     1261 atcgatggaa ttgtcgcggt cagcgcgatc ggcgcccgga tgcggaaaag gatgtcccaa
     1321 gaccgcatgc cgacgctcat cgccgcttcg atctggccct gcggcacggc ggcgaagccg
     1381 gcgcgaaaaa tctccgcgaa ataggccgaa gaatagaggc ccaagccgac aatgccggcg
     1441 gcggtggcgc tcagtctcag gccgatgaac ggaccgcccg catagagcag gaacagttgc
     1501 atcagaaagg gcgtgccgcg caggatctcg acatagacgc gcaggagcca atggagcggc
     1561 gggaatctta gccgattgag caatgtcacg acgaagccga ccgccagacc gatgatcgag
     1621 cccgcgctcc agcagagaac tgtcacgccg aatccgctga gcaacgaagg cagatagtgg
     1681 agaaaaacct ggaggctcac gtcgggcggc cccccgccag ccgccgctcg gcgaagaccc
     1741 cgaaggcggc gaggcagaga ttgatcgcaa gatagaccag ccccccgctg agataggcct
     1801 cgaacggccg gtaggtcatc gacgcgatgt tcatgctgac gcgggtcagt tcggcaatgc
     1861 cgaccaccga caccagcgaa gaggctttga ggagcaggat gaattcattg atcagcggcg
     1921 gcgcggaaat gcgcaaagcc tgcggcagca gaatgcgccg ccagatgtcc gaaggcgcaa
     1981 agccgagcgc ggtcgccgcc tcggcctgac cgcgcggaac cacgttcagc gcgccgcgca
     2041 ggatttccgc ctggtacgcg ccggaggcga agccgagccc gccgatcgcc gccaccacgg
     2101 ccggcacgtc gaggccgtat tcggcgagga aatagtagat gaagagaagc tggacgagca
     2161 gcggcacgcc gcggaagaag ctgacataga agccgccaag acggcggagg gcggcattgg
     2221 ccgagagccg cgccgcgcaa accagcgcgc cgatcgcaaa accgagcgcg atgccggcga
     2281 gcgacagcgc aatcgtcacg cccgccgctt gcagcaaata gcccagcgag tgaaggatga
     2341 tgtcgaaacg catcttagcg tgggctttta acaaaccgtc gtcattgcga ggagcgaagc
     2401 gacgaagcaa tccattcagt gggcgcaacc cgacgtctgg attgcttggc tacgctcgca
     2461 atgacggcgc ttttcatctc aagccatcgc agtcccaaag gaaaaggcgg ccttgaaggc
     2521 cgcctcgcct ctcgccttaa attgtcggct ccggcacggt cttcggcaga tcgccgctct
     2581 ggccgaacca tttcttctgg attttcgcca tggtcccgtc ggcctcggct ttgaggatcg
     2641 ccgcgttgat tgcgtcgatg aaggtcttgt cttcgagccg cccgacccag gagaaatagg
     2701 tcgggtggcc gaaaggcggc aggacaacct tgaacgtgtc ggagcgctgg ttggcggcat
     2761 acgccaaatt gggcagcgaa ttgaccgacg cgtcgatgcg ccccgccgcc agatcggcgt
     2821 aggactggtt gttgtcgaca tattccttga cttcgaccgg cttcggcagc ttggccgcga
     2881 attccttgag ctgggcgagt tgcgaggtgc ccttctgtcc gccgaccgtc ttgccggcaa
     2941 tgtcctcggg cttgttgatc gaattgtcgt tggcgcgctt catcagcgcg gcggtggcgt
     3001 cggcgatcgg cacggtgaac aggtagcgct tcatccgctc cttggtgatc gtcaccggcg
     3061 caatgcaaat atcgaatttc ttggcgtcga ggccgggcag aatgctggtc caaggcagat
     3121 cgagatattt caccttgacg ccgagcgtcg cgccgacggc gtcgacgagt tctcggtcga
     3181 cgcctttata gacgccgttg tcggaaatat cgaaaggcgg gaattgcatt tccgtcgccg
     3241 cgaccagctc gccgcgcgac ttcacgtcgg cgagcgtatc agcgaaagcc cggccgaagc
     3301 cgccgagaaa ggcgccggac gccgccgcgc cgaaaaggcc gtttttaagc aagccgcgcc
     3361 gcgacaggcc gcgcgtatcg aaaatcgtca tgatgtctcc ccttccctct aagaccaaca
     3421 ggccggcggc gatgaaccga gaccgccgat cagcgcctct cgctcgccgc ttgcgaaagg
     3481 gctcctcaaa gattgtccga ctaagcggcg gcggtcgtca aacggccggc cgtcgccgtc
     3541 tcggcttttt attaagtcta gacatattgg gggtcaagcg gcggcggcca gcagccggcc
     3601 cctcccggcg gagcgcctac cccttcacag cgccggcggt caggcccgcc acaatgtgct
     3661 tctgcgcgag caggaacatg atgatcgccg gcaggatggt cagcgtgatg aaggcgagga
     3721 ccaattgcca ggaggtcatg tattcgccct cataggccat catgccgagc ggccaagggt
     3781 agagcgactc gctgttcagc atgatcagcg gcacgatgta attgttccag gaattgacga
     3841 aggcgatgat cgcgacggtg gagagaatcg gccgcgacag cggcagggtc acatagagaa
     3901 agtagcggat atagccgcag ccgtcgacca aagcggcgtc gagcaactcg ctcggcaatt
     3961 gccggaagcc gttgcggagc aagaggaccg ccatcgccag gtagaaagcc gactgcggca
     4021 ggatcacgcc ccagggcgta tcgagcaggc cgagatcgcg cacgcggatg aacagcggca
     4081 ggatcgcggt ggcgatcgga aacatcaggc cgagcgtcat ataggtgaag aggaagcggc
     4141 tgccgaaaaa cttcagatgc gcgaaggcga aggcggccat ggccgacagc gcgacggtga
     4201 tcgccacggt gaagcaggcg atgaacagcg aattgccgag cacgcgccag tagcgcgcgc
     4261 tttcgagaat gtcgagataa ttgcgccagc gccatggatg cggcaagccg agcgggttgg
     4321 tgcgcagctc gccgagctcc ttgaagccgc cgatcgcggt cgcgagcagc ggcagaagga
     4381 cgacgctcga gacgaggagc agcaccgcca gccggaacag cggatagagg tcgaacggct
     4441 tttggccgcg cgagcgccgc ctcgtcctgg tgaggctcgc aggcgcgaag agggttagtt
     4501 gctcactcat cgcgcatcaa gagccgtctg tagccaaaag cgaagctcac gcaaatgatg
     4561 aacagaacga cgccgaccgc cgagccgaag ccgacccgca tgcgcgtgat gccgaaaaga
     4621 taaaggaacg agaccatcgt gttggtggcg ttgaacgggc cgccgccggt cagcggcacg
     4681 acgacgtcga agaactgcag cgcgccgatg accgcaaaga acaccgacag cttgatcatc
     4741 ggcgagagct gcggaatcgt cacgaagcga aagcgctgga aagcgccggc gccatcgatc
     4801 tcggccgctt cgtaaaggtt tctgtcgatt ccctgcaggc cggcgatgaa cagcatcatg
     4861 tggaagccga aatacttcca gacgatgacg acgagcagcg cgaccatggc ccaggtcggg
     4921 ctcgccagca tatagacggg gccgaagccg agcgcctggc tcgcgccgga aagcggcccg
     4981 taatcgccgt cgaagatgaa gcgaaaaatc aggccggcgg ccacgtcggc gagaatgtaa
     5041 ggcaggaaga agatcaggcg gaaagcaacg gcgccgaagg cgcgcttggc caaaagcagc
     5101 gccatggcga gcgcgagcgg cagttgaatc gccagcgaga cggcgatgat gatcagattg
     5161 ttcagcgccg cggttctgaa attcgaattg ttgaagagcg aaatgtagtt cttgaggccg
     5221 atgaaatttt gcggccggtt gtagccgtcc caattgtaga aaccgtacca cgccgcctcg
     5281 ccgatcggca ggatgacgaa aacggtgaac agaagcagag ccggcggcag aaagatcagc
     5341 gccgcgaccc atacgccgcc cgccgcgctt ttgctctcct gtacggtggt gacggtcatc
     5401 gcctacccaa attcgggggg agttcccttc tccccgcaat gcggggagaa ttcgggggag
     5461 aatcccttct ccccgcttgc gaggagaagg tggcgcgaag cgccggatga ggggctgcgc
     5521 aggactttcg aaaccagcgc tctgtgaaac gctaatccca aacttcagcg ccgcccctca
     5581 tccggccgag cttcgctcgg ccaccttctc cccgcaaggc ggggagaagg gttactcgcg
     5641 ccccctattg ctcgtcccag gcctgctgca cggcggcggc gccgtcttcc ggcgacattt
     5701 tgccggccgc caccgcgacc gatatgtcgt tgacgacgcg gccgaccgac gggccgagat
     5761 cctgatcgaa gaagttctga tgatacgtgg tcttcgcgag atcggcgccg atctgcttga
     5821 ggaccggatt ttcgatcgcg ctttccgcgc ccttgacgac cggaatatac aggcccttgg
     5881 cggcggcttc gcgctggtac ttctcctggc tgaagaattt caggaactcg gccgcctctt
     5941 tcggcgcgcc cttgccgacc agccagccat tgatgccgcc gagcgtatcg gtcggctggc
     6001 ccttgccgcc gggcagggtc gggaagctga aaatgccgat attgtcgatc ggctggccct
     6061 tgccgtcggc ggcgttcggg ccttgcgcgg tcaacagcca ctggcccatc agctccatcg
     6121 cccctttgcc gtcgccgaac atgccttcgg cttgcggatg gctcgtcgat aggtagccgg
     6181 cctggaaggg ttcgagcgcg gcgagttcat gaaggcgctt gcccgcctcg acgaaggtcg
     6241 cgttcttgaa gccgccgtcc ttgcccgcct tggcgtccgc cagcgcgttc gcgccgccgc
     6301 cgcggatgac gagataggac cagtagaaat gcatcggcca tttctcgccg ccgccgacga
     6361 tgatcggcgt gatcccggcc gccttgatct tcttcacggc gcccaggaag tcgtcccagg
     6421 tcttgatgtc ttcggccttc acgcccgctt tggcgaagag cttcttgttg tagaagaaga
     6481 ccacctcgcc ggtgttctgg ggaatgccat aggtcttgcc gtcgacctga aacgcctcga
     6541 ccgcggtcgg cgatatggtg ttggcccagg gcgcggccgc ggcggtgatg tcctcgagga
     6601 aacccgcctt cgcctgcgcg cgcatgacgc cgccgcccca actgtagaag atgtggggcc
     6661 gatccttgtc ggattgcagc atggtcggca gcttggcctt gaaggcttcg ttctccaggt
     6721 cctggagctg gattttgacg cctttgttct gcgcctcgaa atcgtgaatg atctggttgt
     6781 agagcgccat ggtgccggcg tcgtcgttcg atagatgcag ccagcggatg gtcgtgtcgg
     6841 cgcaagcggc gctcgctcca aaagcgaggc aaaaggacgc gagcgcaaga gtccggaact
     6901 tcatagtctc ctccccgcag caggtaagct aggcgtatcc cgtttttttg cggtatgcgt
     6961 attaaatctg accggagttt tccgaactgt caaggacgat cgggattctc agcggaacgg
     7021 cgcgctttcg cccattgaca ccacgcgccc ggagtgaaat ttatcctgaa cccaaataaa
     7081 tatccgtccg agcgggacct agacgcgcct caatgaagac agccgatccc gaactgatgc
     7141 gcgccatcaa tcggttccat gtgatggatt cgatccggcg ccatggcccg atttcgcgcg
     7201 tcgaaatttg cggccgcacc gatctgagcc cgaccaccgt ctcggcgatc accgccgctt
     7261 tgatcgagga tggattgatc gtcgcgcggc cgatcggcga cgtacgcgac gcgacgcgcg
     7321 ggcgcccgcg cgtgatgctc gagctcaatc ccgaagcggc ctatgtcgtc ggcgtcaagt
     7381 tggcgccgga ccagatcacc atcgccgcga ccaatttccg cgccgacgtg ctggcgagct
     7441 tggcgctgcc gatccggatc agccggcagc cggcctccgt cgtcgccgat ctcgtcgagg
     7501 acggcgtgcg gcgctgcctc gccgatgctg acttgacggt cgcgcgcatt gccggcctgt
     7561 gcgtcggcct gcccggcgtg gtcgagcggg cgagcggcgt ctgccgccag agtacggtgt
     7621 tcggcgagcg cgatctgcct ttcgccaagg agctttccgg ccggctcggc ctgccggtgt
     7681 cgatcgatac agacgtcaac ctcgtcacgc tggccgagca ttggttcggc gaggtgaaag
     7741 gcttgagcga ttttctcgtc gtcagcatcg aggacaatct cggcctcggc atcatgcata
     7801 atggcgagct gtttcgcggc gcgggcgggc tttcgcccga tctcggcgac cttctcgtcc
     7861 gtcccaacgc cggcgacggc cgcaaccgcc tcgcctcgat cgcctccgaa accgcgatcg
     7921 tcggggaggc gcttgacgcc gtcgacggga aacgcgaccg cttcgcgaaa atcggcaagg
     7981 agctcgacaa ggtcatcgag ctcgctcagg aaggcgacca gcgcttcatc ggcattctcg
     8041 cggcggcggg cgacgcgctc ggcttcgcga tcgccaatct gatcacgctg ttcgcgccgc
     8101 ccaaggtcat tctcggcggc gccgcggtca aggctgggcc gctcttgctg gagccgctgc
     8161 gcgagaccgc ttcgacgctg gttccggcga gcctcgccca tgtcgccgaa atcgtggtcc
     8221 atcaatggag cgacgagatg tgggcgcgcg gcgccgcggc gatgacgctt cgcgacctct
     8281 atggcgcgcc ttggaatacg accgggccgg cgctgctgcg ctgaatgaga tgattcgggg
     8341 agatacagcg tgaaacgcgt cggcattggc gtcattggct gcggctatat cagcgccgct
     8401 tatctgaaag ccgcgcggct cttcccgatc ctggaggtac gcgcgctcgc cgacctcaac
     8461 cgcgaggcgg cggaagcgcg cgcgaaagaa ttcggcttga aagccgtgtc gatcgaaaag
     8521 ctgctgagcg atccgaagat cgagatcgtc ctcaatctca ccattccgaa agcccatgtc
     8581 gcggtcggcc tgcaggcgat cgaggccggc aagcacgttc attccgaaaa gccgctcgga
     8641 atcgcggtcg ccgaggcgaa gaagctgatc aaggcggcga aaatccgcgg cctgcgcatc
     8701 ggctgcgccc ccgacacatt ccttggcggc gcgcaccaga cctgccgcaa gctgatcgat
     8761 aacggccgga taggcacgcc ggtcggcggc acggccttct tcatgtgccc gggccatgaa
     8821 cgctggcatc ccaatcccga tttctactac gagaaaggcg ccgggccgat gctcgatatg
     8881 gggccgtact acattacggc tttggtcgac ctgctcgggc cggtggcccg ggtcgccggc
     8941 gtcgcgacca agctgcgcaa ggagcgggtc atctccagcg agccgcggcg cggcgagacg
     9001 attgcggtca gcacgccgac tcacattgct ggaacgctgg aattcgccag cggcgcggca
     9061 atttcgatcg tcatgagctt cgatgtgccg aagcacgggc acaagccgct cgaaatctat
     9121 ggtacgaaag gctcgctgat cgtccctgat ccgaatttct tcgacggcca gatcgaaatc
     9181 gccgccgccg gcgaggactg ggggcccgtc gcgaccgagc acgcgtacgc cgacggcaac
     9241 taccggatca tcggcgcggc cgacatggcg caggccattc gcgccggccg tccgcatcgc
     9301 gccaacggcg atctcgcctt ccatgttctc gaagtgatgg aggcgttcgg gcgctcgtcc
     9361 aagtcgggcg ctttcgtcga gatcaaaagc cggcccgagc gccccgcccc cctgcccgtc
     9421 ggccttaaga ccggcgagct cgactgaagc cgcctcaagg atcgaaagga gttcaacgat
     9481 gcgggaagcc ttgatcgtct ggggaggatg gagcggccac gagcccgaga tcggcgcgca
     9541 tatcattgcc gacatgctca agaaggaggg gtttgcgacc tatatcgaaa acacgacgga
     9601 agccttc
//
LOCUS       DATGJW010000315         2964 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_472535, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000315 DATGJW010000000
VERSION     DATGJW010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2964)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2964)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2964
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_472535"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(215..1249)
                     /locus_tag="VKU03_09655"
     CDS             complement(215..1249)
                     /locus_tag="VKU03_09655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ71572.1"
                     /translation="MAIPHPTRRNVLRGGALAAAGLALPTLVASRGAVAAGTQKVDMQ
                     LGWIVGGNQLGEVVAKRLGYFEEEKIAFAIQPGGPSIDGVAIVASGRYEIGQVSSSPS
                     LMLAASQGIPVTCFAIGAQKHPYCFFSLPKAPIRKAEDMIGKRIGVQATGQILLSALL
                     RKHNIPADKVEKVIVGSDMTPLVAGQVDAITGWRTNTTALKVLGPDYISMSLWDQGVR
                     LYALPYYATADTLHSKAELLAGFLRAAGRGWAYAKENPEKAVDLLVKEYPNLVKADEL
                     VAAAIMLSYEFTDRTKTYGWASFDPAVWEEQIDLFDALKQFTAAKPKLDQVMTMAILD
                     ATKDARPKIG"
     gene            1396..2343
                     /locus_tag="VKU03_09660"
     CDS             1396..2343
                     /locus_tag="VKU03_09660"
                     /inference="COORDINATES: protein motif:HMM:NF015431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ71573.1"
                     /translation="MHAVVLRYLDAVARLGSIRRAAESLNVASSAVNRQILKLEAEIG
                     ASLFERRGNGVKLTAAGDYMLRHSRATLADWSKTRAEIAALAGDIRGEVRLISIPSLL
                     VSVVPQAINTLAKSHPNISFRVVDADPEIHVAEMRAARPDIATLFIDRRHRQYEIVTR
                     LRLALGAIMRPDHPLARAGSLTLTQCAAYPVVMLNDPWLLDAASETEFIRSGAQFRAR
                     LKSNSLILMKQAIRDGLGIGFFTPTGFVDELASGELIHVPLAERGLANSEIGLLVHRE
                     RLSTPPISLAAEALARAFEDFGARLAQTARPARIGARRA"
     gene            2400..2864
                     /locus_tag="VKU03_09665"
     CDS             2400..2864
                     /locus_tag="VKU03_09665"
                     /EC_number="1.11.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016102133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004602 - glutathione peroxidase activity
                     [Evidence IEA];
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione peroxidase"
                     /protein_id="HLJ71574.1"
                     /translation="MALRTIDGREETFGAYAGKARLVVNVASKCGFTPQYQGLEALYR
                     KYRDRGFVVLGFPCDQFGHQEPGDDAAIRAFCSDQYDVSFPLFAKIEVNGANAHPLYR
                     ALKRIAPGLLWTKAIKWNFTKFLVDREGASVKRYAPRVKPAAIAADIERALG"
BASE COUNT          509 a          961 c          955 g          539 t
ORIGIN      
        1 ggtggcttac gggctgcgat ttaagaccga aaggcgaagg gccgctgtga tgaaaatcac
       61 agcaaaattt tattccgctc aactgcctac gccgtcattg cgatgagcga ggcgacgaag
      121 caatcaagtc atggagctgc gccctacgag tggattgctt cgcggcgctc gcaatgacgg
      181 cgtattttgt tggccgactc atcttcacct ccattcatcc gatcttcggc cgcgcatcct
      241 tggtggcgtc gaggatcgcc atcgtcatga cctggtcaag cttcggcttg gcggcggtga
      301 attgcttcaa ggcgtcgaaa aggtcgatct gctcttccca aaccgccgga tcgaaacttg
      361 cccacccgta cgtcttggtg cggtcggtga attcgtagga gagcatgatc gccgcggcga
      421 ccagctcgtc ggctttcacg agattgggat attccttgac gagcagatcg accgcctttt
      481 ccggattctc cttagcataa gcccagccgc ggccggcggc gcgcaggaat ccggcgagaa
      541 gctcggcctt ggaatgcagc gtgtcggccg tcgcatagta gggcagcgca taaaggcgca
      601 cgccctggtc ccagagcgac atcgaaatat agtccggccc cagcactttc agcgcggtcg
      661 tgttggtgcg ccatccggtg atcgcgtcga cctggccggc gaccagcggc gtcatgtccg
      721 agccgacgat caccttctcc actttgtcgg cgggaatgtt gtgcttgcgc aacagcgcgc
      781 tgagcaggat ttgcccggtc gcctgcacgc cgatcctctt tccgatcata tcctcggcct
      841 tgcggatcgg cgccttgggc agtgaaaaga aacagtacgg atgtttctgc gcgccgatgg
      901 cgaagcaggt gacgggaatg ccttgggaag ccgccagcat cagagacggg ctcgacgaga
      961 cctggccgat ctcgtagcgg cccgaggcga cgatggcgac gccgtcaatg ctcggcccgc
     1021 ccggctgaat ggcgaaggcg atcttctctt cctcgaaata gccgagccgc ttggcgacga
     1081 cctcgccgag ctggttgccg ccgacgatcc agccaagctg catgtcgacc ttctgcgtgc
     1141 cggccgcgac ggcgccgcgc gaggcgacca gggtcggaag agcgaggccg gcggcggcca
     1201 gcgcgccgcc tctcaggacg ttgcggcggg tgggatgggg gatcgccata gggagtttcc
     1261 tcgtcaagcc gcggcgctcc gttcagcgac gccgtcagcg gaccggccga tttaacgata
     1321 gtcaggtgag cgaaggaagc atatattttc gcgatggccg ctgccttttc ggcagcgccg
     1381 ccgcggaacc cgatcatgca tgcggtcgtg ctgcgctatc tcgatgcggt cgcccggctg
     1441 ggctcgatcc ggcgggccgc cgaatcgctg aacgtcgcct cctcggccgt caaccggcag
     1501 attttgaagc tggaggcgga gatcggcgcc tcgctgttcg agcgtcgggg caatggcgtc
     1561 aagctgaccg cggccggcga ctatatgctg cgccattcgc gcgcgacgct cgccgattgg
     1621 agcaagactc gcgccgagat cgcggcgctc gccggcgaca ttcgcggcga ggttcggctg
     1681 atctcgattc cctcgctcct cgtcagcgtc gtgccgcagg cgatcaacac gctcgccaaa
     1741 agccatccca atatcagctt ccgcgtcgtc gacgccgatc cggaaatcca tgtcgccgag
     1801 atgcgggcgg cgcggcccga catcgcgacg ttgttcatcg accggcgcca tcgccagtac
     1861 gagatcgtga cgcggcttcg cctggcgctc ggcgcgatca tgcggccgga ccatccgctg
     1921 gctcgcgccg gcagcctgac gctgacccaa tgcgccgcct atccggtcgt gatgctcaac
     1981 gatccctggc tgctcgacgc cgcctcggag acggaattca tccgcagcgg cgcgcagttc
     2041 agggcgcggc tcaagtccaa ttcgctgatc ctgatgaagc aggcgatccg cgacgggctc
     2101 ggcatcggct tcttcacccc gaccggcttt gtcgacgaat tggcgagcgg cgagctcatc
     2161 catgtgccgc tcgccgagcg cggcctcgcc aatagcgaga tcggcctgct ggtccatcgc
     2221 gagcggctgt cgacgccgcc gatcagcctc gccgccgagg cgctggctcg cgccttcgag
     2281 gatttcggcg caaggctggc gcaaaccgcc aggccggcgc gaatcggcgc ccgccgagcc
     2341 tgacggcgcg ccgctttcaa gcccccatgg agctctcatt gaccagcccg ctcgacgcca
     2401 tcgccctgcg cacgatcgac gggcgcgagg aaacattcgg cgcctatgcc ggcaaggcgc
     2461 ggctcgtcgt caatgtcgcc tcgaaatgcg gcttcacccc gcaatatcag ggactggagg
     2521 cgctctatcg gaaatatcgc gaccgcggct tcgtcgtgct cggctttccc tgcgatcagt
     2581 tcggccatca ggagccgggc gacgacgcgg cgatccgcgc cttttgttcg gaccagtacg
     2641 atgtgagctt tccgctcttc gccaagatcg aggtcaacgg cgccaacgcg catccgctct
     2701 atcgcgcgct gaagcgcatc gcgccgggcc tgctctggac gaaggcgatc aaatggaact
     2761 tcaccaagtt cctggtcgac cgggaaggcg cgagcgtcaa gcgctacgcg ccgcgcgtga
     2821 aacccgcagc gatcgcggcg gatatagaac gggcgctggg ttgaacctcc ccttctcccc
     2881 gcgaagcggg gagaaggtgg cccgaggggc cggatgagca tctgtgatgg aagtcaaggt
     2941 cgatttggct gccaggttcg attt
//
LOCUS       DATGJW010000316         4359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1510743, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000316 DATGJW010000000
VERSION     DATGJW010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4359
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1510743"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VKU03_09670"
     CDS             complement(<1..412)
                     /locus_tag="VKU03_09670"
                     /EC_number="3.6.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005777 - peroxisome [Evidence IEA];
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0003986 - acetyl-CoA hydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0008413 - 8-oxo-7,8-dihydroguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0035539 - 8-oxo-7,8-dihydrodeoxyguanosine
                     triphosphate pyrophosphatase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0009132 - nucleoside diphosphate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0015938 - coenzyme A catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HLJ71575.1"
                     /translation="MNAAPPAFFSAEDFRARARARLSLDLPEDALNPLIAPRRGDFAL
                     NALTPDEHAVGAARPAAVLAPIIARPDGATVLLTQRAAALRQHSGQVAFPGGKIEDKE
                     TPLQAALREAMEEIGLDERHIEPTGWLDPYLTGTG"
     gene            complement(409..1005)
                     /locus_tag="VKU03_09675"
     CDS             complement(409..1005)
                     /locus_tag="VKU03_09675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011471395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HLJ71576.1"
                     /translation="MADRLAPRPPEQALHGLEAASAIKKRGPAPVHLWNPPYCGEIDM
                     RIAADGTWFYNSSPIGRLALVQLFASILRRDEDRYVLVTPVERVGIRVDDAPFLAVEM
                     KVEERPEGRRLSFRTNVEDVVVADRDHPLRFERGAAAGLKPYVLVRDKLWALVKRALF
                     YDLVALGETRRHGGEDWFGVFSDGIFFPMAPAAELDVS"
     gene            1273..2286
                     /locus_tag="VKU03_09680"
     CDS             1273..2286
                     /locus_tag="VKU03_09680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLJ71577.1"
                     /translation="MTSLQDAVVANIDEAVARTADAALEKIAAARSAIGAVIFGQDKV
                     VEQALVTLLSGGHGLLIGVPGLAKTKLVETLGVVFGLDSRRVQFTPDLMPSDILGSEV
                     MEEASDHSRSFRFIRGPIFAQLLMADEINRASPRTQSALLQAMQEHHVTIAGARHDLP
                     RPFHVLATQNPLEQEGAYPLPEAQLDRFLMQIDVDYPDRIAERRILIETTGEAETKAK
                     PAMTAEDLMEAQRLARRIPVGESVVETILDLVRAARPGEGDAETTKAISWGPGPRAAQ
                     ALMLASRARALVEGRLSPSKDDIAALAEPVLKHRMALTFAARAEGETVTAVIARVVAK
                     TVG"
     gene            2446..3381
                     /locus_tag="VKU03_09685"
     CDS             2446..3381
                     /locus_tag="VKU03_09685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLJ71578.1"
                     /translation="MTAAARAPEKSAIGQARWRSEAASLAARLPSLVVAARQLAQSVM
                     HGVHGRRRAGPGENFWQFRPFLSGEPAAAIDWRRSAREDRAYVREREWEAAHTVWLWT
                     DRSASMRFASPMAQAAKIDRAVVLGFAAADLLVRGGERVGLIGLTRPLATRGVIERFA
                     EAVAVDEGLNATRAPLPPAQPLKAHSKAILIGDFLSEDEAIRRCINALSAEGAEGHVL
                     MIADPIEEAFPFEGHTEFVAADGALRLRAPRAQGMRQDYLERLQRHREAVRETCRDRG
                     WSFAIHRTDRPAAEALLALRMRLEGPTSRLSAGGA"
     gene            3383..>4359
                     /locus_tag="VKU03_09690"
     CDS             3383..>4359
                     /locus_tag="VKU03_09690"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HLJ71579.1"
                     /translation="MVGLPLAFTAPAALIALVLLAALYVFLRITPPRPRQTVFPPLRL
                     LFGIERKEQTPARTPWPLLALRLAIAAAVILAMAGPIWNPLLFGGGAGPLLMIVDDGW
                     AAAPSWDARKTLINSRLQGAAHDNRLAVLLPVSQGGVDIAPADAARNSEKLRALEPQP
                     YAPDRMAILPPIRKFLAEFPRAEIVWLADGVSLGQADPFAHELAALAKGHEATVLLGK
                     DTALALTSVEDVSGALEAKALSAGPSNRKGMLRALDAKGLTIAEAPFDFAGKDTAVAN
                     FDVPTELRNQISRLAIDGERSAGAVLLLDESSRRRRVAVASGVSADVAQP"
BASE COUNT          658 a         1509 c         1491 g          701 t
ORIGIN      
        1 agccggtgcc ggtgaggtac ggatcgagcc agccggtcgg ctcgatatgg cgctcatcga
       61 gcccgatctc ctccatcgcc tcgcgcagag ccgcttgcag cggcgtctcc ttgtcttcga
      121 tctttccgcc gggaaaggcg acttgcccgg aatgctggcg gagcgcggcg gcgcgctggg
      181 tcaaaagcac ggtcgcgccg tccggccgcg cgataatcgg cgcgagcacc gcggccggcc
      241 gcgccgcgcc gaccgcatgc tcgtccggcg tcagcgcatt cagcgcgaaa tcgccgcggc
      301 gcggcgcgat cagcgggttg agcgcgtctt cgggcaaatc gagcgacagc cgcgcgcggg
      361 cgcgggcgcg gaaatcctcg gccgaaaaga aagcgggcgg ggcggcgttc acgagacgtc
      421 gagctccgcc gcaggcgcca tcgggaagaa aatcccgtcc gagaagacgc cgaaccagtc
      481 ctcgccgcca tgtcgccgcg tctcgccgag cgcgacgaga tcgtagaata gcgcccgctt
      541 gaccagagcc cagagcttgt cgcgcaccag cacatagggc ttcaggcctg ccgcggcgcc
      601 gcgctcgaaa cgcagcgggt ggtcgcgatc ggcgacgacg acatcctcga cattggtccg
      661 gaagctcagg cgccgccctt ccgggcgctc ctcgaccttc atttcgaccg ccaggaaggg
      721 cgcgtcgtcg acgcgaatgc cgacccgctc gaccggcgtg accagcacat agcgatcctc
      781 gtcgcgccgg agaatggagg caaagagctg gaccagggcc aagcggccga tcggcgagga
      841 attgtaaaac cacgtcccgt cggcggcgat gcgcatgtcg atctcgccgc aatagggcgg
      901 attccacaaa tgcaccggcg cgggcccgcg ttttttgatc gccgaggcgg cctcgaggcc
      961 gtgcaaagcc tgctcgggcg ggcgcggggc gagccgatca gccatttgtg aagagccgtt
     1021 cgccatattc tcgcgcttct ttgtgggcaa gcatccggcg ccgccaaagc ggctttccgg
     1081 tttcgccgcg ctcgccctaa tctaagcagg tttccctggt tgaccaatgt cggcgcggcc
     1141 tcgcttcctt cgagactgcg ctcccgagag gagggcgcgg acgctgcgcc catcatttgc
     1201 cggcgcaagc ttgggtctct atggaaccgt cgaggggaaa cttcgcccgt caacccgcgt
     1261 ttgggagatt cgatgacttc gcttcaagac gccgtggtgg cgaatatcga cgaggcggtg
     1321 gcgcgcacag ccgacgccgc cttggagaag atcgcagccg cccgcagcgc catcggcgcg
     1381 gtgattttcg gccaggacaa ggtggtcgag caggcgctgg tcaccctgct ttccggcggt
     1441 cacggcttgt tgatcggcgt gccgggcctc gccaaaacca agctggtcga aacattgggc
     1501 gtggtcttcg ggctcgattc gcgtcgcgtg caattcaccc ccgatttgat gccgtccgac
     1561 attctcggct cggaagtgat ggaggaggcg agcgaccaca gccgctcgtt ccgcttcatc
     1621 agggggccga tcttcgcgca attgctgatg gccgacgaga tcaaccgggc cagcccgcgc
     1681 acccagtcgg ccttgctcca ggcgatgcaa gagcatcacg tcaccattgc cggcgcacgc
     1741 cacgacttgc cgcgcccatt ccatgtcctg gcgacgcaaa acccgctcga gcaggaaggc
     1801 gcctatccgc tgcccgaggc gcagctcgac cgtttcctga tgcagatcga cgtggattat
     1861 cccgaccgca tcgccgagcg ccggattttg atcgagacga ccggcgaagc ggagacaaag
     1921 gctaagccgg cgatgacggc cgaggatttg atggaggcgc agcgcctcgc ccgccgcatt
     1981 ccggtggggg aaagcgtcgt cgagaccatc ctcgacctgg tccgggcggc gcggccgggc
     2041 gagggcgacg ccgagacgac caaagcgatc tcctggggac cgggtccgcg ggccgcccag
     2101 gccttgatgc tggcgagccg cgcccgggcg cttgtcgagg gccggctgtc gccgtccaag
     2161 gacgacatcg cggctttggc cgagccggtg ctgaagcacc gcatggcgct gactttcgcc
     2221 gcccgcgccg agggcgagac ggtgaccgcg gtgatcgccc gcgtcgtcgc caaaacggtc
     2281 ggctgagtag gctcttttgg ctgaccggca catgcggcgg ctcgccctca ccccggccct
     2341 ctcccgcaag cgggagaggg tgcgcgtctt catcagcagc cgcgctgtcg ccgcggtcgg
     2401 cgaccgcggc tgtaattctt tccgcttgcg ggatgagggt tgtccgtgac cgccgccgct
     2461 cgcgcgccgg agaaatcggc gattggccaa gctcgttggc gcagcgaagc cgcctcgctc
     2521 gcggcgcgct tgccgagcct ggtcgtcgcc gccaggcagc tcgcgcaaag cgtgatgcac
     2581 ggcgtgcatg gccggcgtcg agccgggccc ggcgagaatt tctggcagtt ccggcctttc
     2641 ctttccggcg agcccgcggc ggcgatcgac tggcgtcgct cggcccgcga ggatcgcgcc
     2701 tatgtgcgcg agcgcgaatg ggaggcggcc cataccgtct ggctgtggac cgaccgctcg
     2761 gcgtcgatgc gcttcgcttc gccgatggcg caggcggcca agatcgaccg cgccgtggtg
     2821 ctcggcttcg ccgccgccga ccttctggtg cgtggcggcg aacgggtcgg cctgatcggc
     2881 ctgacccggc ctttggcgac gcgcggcgtc atcgagcgtt tcgcggaagc ggtcgctgtc
     2941 gatgagggcc tgaacgcgac gcgcgcgccc ctgccgcccg cgcaaccgct gaaggcgcat
     3001 tccaaggcga tcctgatcgg cgatttcctc agcgaggacg aggcgatccg acgctgcatc
     3061 aatgccctct cggcggaagg cgccgagggc catgtgctga tgatcgccga tccgatcgaa
     3121 gaggctttcc ccttcgaagg ccataccgaa tttgtcgccg ccgacggcgc gctgcgcctg
     3181 cgcgcgccgc gggcgcaagg tatgcggcaa gattatctgg agcgcttgca acgccatcgc
     3241 gaggccgtgc gcgaaacctg ccgcgaccgc ggctggagct tcgcgatcca tcgcaccgac
     3301 cggccggccg ccgaagcgtt gctcgccttg cgcatgcggc tcgaagggcc gacgtcgcgc
     3361 ctttccgccg gcggggcgta acatggtcgg cctgcctctc gccttcaccg cccccgccgc
     3421 cctcatcgcg ctggttctgc tcgccgcgct ctacgtcttc ctgcggatca cgccgccgcg
     3481 cccacgccaa acggttttcc cgccgctgcg cctgcttttc ggcatcgagc gcaaggagca
     3541 gacgccggcg cgcacgccct ggccgcttct ggctctgcgc ctcgccatcg cggccgcggt
     3601 gattctggcg atggccggac cgatctggaa tccgctgctt ttcggcgggg gcgccgggcc
     3661 tctcctgatg atcgtcgacg acggctgggc cgccgcgccg agctgggacg cgcgcaagac
     3721 gctgatcaat tcgcgcttgc agggcgccgc gcacgacaac cgcctcgccg tcttgctgcc
     3781 ggtctcgcag ggcggcgtcg atatcgcgcc ggccgatgcg gcgcgtaatt cggagaagct
     3841 ccgcgcgctc gagccgcagc cttacgcgcc tgaccgcatg gcgatcctgc cgccaattcg
     3901 caaattcctc gcggaatttc cgcgcgccga aattgtctgg ctcgccgatg gcgtctcgct
     3961 cggtcaggcc gaccctttcg cgcatgagct cgccgcgttg gccaagggcc acgaggcgac
     4021 ggtgcttctc ggcaaggaca cggcgctcgc cttgacgagt gtcgaggatg tcagcggcgc
     4081 gctcgaagcc aaggcgctga gcgccgggcc gagcaaccgg aagggcatgt tgcgagcgct
     4141 cgacgccaaa ggcctgacga tcgctgaagc gccgttcgat ttcgccggca aggatacggc
     4201 ggtcgcgaac ttcgacgtcc cgaccgagct gcgcaatcag atctcgcgcc tcgcgatcga
     4261 cggcgagcgt tcggccggcg cggtgctgct gctcgacgaa agctcgcggc ggcgacgcgt
     4321 cgccgtcgcg tccggcgtct ccgccgacgt cgcgcagcc
//
LOCUS       DATGJW010000317        11310 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1098858, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000317 DATGJW010000000
VERSION     DATGJW010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11310)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11310)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11310
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1098858"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..511
                     /locus_tag="VKU03_09695"
     CDS             <1..511
                     /locus_tag="VKU03_09695"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71580.1"
                     /translation="VGRAPPDFLRLYDLKLGPAPLLVIFLAALTLAGIFVLRRTAFGR
                     YIYAIGGNEQVALLSGIRVDRVKIGAYMLCSTLAALTGLLYLARAGIGDPVTGEGAEL
                     QAITAVILGGTSLFGGRGGLVGTLGGVLLMGLTNNVLVILNVSSWYQELIQGLVIVGA
                     VALYKQKGR"
     gene            complement(790..1749)
                     /locus_tag="VKU03_09700"
     CDS             complement(790..1749)
                     /locus_tag="VKU03_09700"
                     /inference="COORDINATES: protein motif:HMM:NF013335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleoside hydrolase"
                     /protein_id="HLJ71581.1"
                     /translation="MSDICRMILDVDTGVDDAMAILYALNRPGIKLEALTTTFGNTDI
                     DSATGNTLRILELAGRSDIPVARGPGRSLMKPFAKGAEHVHGRNGLGDLALPEPKAKP
                     VGEHASDLMIRLARENPSEITLCPVGPVTNVAIALAKAPEVARLFKQIVIMGGTIRHP
                     GIQGVPTPTASANVWNDPEAAQIVMRSGAKITLVGLDVTMKALLTAAMRKSIAESGKV
                     GATMMQIADFYVRAYESMHPGIAGCGLHDPLAVAIAEDKSLATVERMCVDIELYGAFT
                     RGRVIVDRRRNAAAERFNADVCVDVDGARFSRLFVETLAGFVT"
     gene            complement(2036..2120)
                     /locus_tag="VKU03_09705"
     tRNA            complement(2036..2120)
                     /locus_tag="VKU03_09705"
                     /product="tRNA-Tyr"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(2084..2086),aa:Tyr,seq:gta)
     gene            2474..3181
                     /gene="rlmB"
                     /locus_tag="VKU03_09710"
     CDS             2474..3181
                     /gene="rlmB"
                     /locus_tag="VKU03_09710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009451 - RNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA
                     (guanosine(2251)-2'-O)-methyltransferase RlmB"
                     /protein_id="HLJ71582.1"
                     /translation="MQIYGFHSVEAALKAPRRRLIRLYATPAAAARLAAEAAARGVET
                     VVMSNEEIALRAPRQAAHQGVLLEARPLAPIDLAEIPERGLVVVLDQVTDPHNVGAIL
                     RTGAAFAIDALVTTERHSPELTGALAKSASGGLEHVPVCSVVNLARALQELGDRGYWR
                     VGLDSEGPQSLSDMALSRPLALVLGAEDKGLRRLTRENCDALARLDMPGPIKSLNVSN
                     ACAVALTIARAQLGEAT"
     gene            complement(3218..4930)
                     /gene="mltG"
                     /locus_tag="VKU03_09715"
     CDS             complement(3218..4930)
                     /gene="mltG"
                     /locus_tag="VKU03_09715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HLJ71583.1"
                     /translation="MGDGSINDGSGGDRPRVSLFGRRATPRSPTEALQPQAAPPPPEP
                     PRKERREGLSRLSGVLSFLLIAAIVGIGVFAWALQEARRPGPLPADKVVILSREDEAD
                     GATIADQLEKAGVINSTLWFNVVVLADGNRGKLKRGEYLFKARASLREVENTLVSGKP
                     VQHKMTIPEGLTSQQVVQRLRADDVLVGDIRETPREGSILPDTYFFTRGESREALLAR
                     MEKAQAKVVDEIWQARAANLPIKSPGELVTLASLVEKETGKADERPRVAAVFIHRLKK
                     RMKLQSDPTIVYGLVGGKGTLDHPITKSELDQPTPYNTYVIEGLPPGPIANPGKAALE
                     AAANPADTNDLYFVADGTGGHAFAETLDQHLKNVDHWRQIERDAKEAKDRVAPDLLPI
                     MPASAQPGPRPKKSELDVSPKVFGALVPRITTKPAPASLASRLARIGAERQSRLALLG
                     PNGPLSAVATGKNLADSGLVVEGVNDATPEDPTAAAPAQTYPLSPQMLAEQRSREARY
                     GEAAPPPGKMANAETNSALLSKPAANGRPRIIDASEGTALDPLLNTTYDLNYPKVVPP
                     LRLQ"
     gene            complement(5022..6281)
                     /gene="fabF"
                     /locus_tag="VKU03_09720"
     CDS             complement(5022..6281)
                     /gene="fabF"
                     /locus_tag="VKU03_09720"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013913051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HLJ71584.1"
                     /translation="MKRVVVTGLGMVTPLACGVEATWRRLLAGESGVGRVENFDVSDL
                     PCQIAAQVPRGPLSEDRFNPDDWMEPREHRRVDDFIVYSMAAATQALKDANWAPSSYE
                     EEIETGVMIGSGIGGLGGIYEASMVLKERGPRKMSPFFITGRLINLTSGYVSIAHKLK
                     GPNHSVVTACSTGAHAIGDAGRLIALGDANVMVAGGAESAVNRLGIAGFAACRALATS
                     FNDRPTKASRPYDRDREGFVMGEGAGCVVLEELEHARARGARIYAELVGYGLSGDAHH
                     ITAPAEDGDGAFRAMTAALKRANLAPADIDYVNAHGTSTPPGDAIELRAVERLMGNAA
                     GALTMSSTKSSIGHLLGAAGAVEAIFCILAMRDGIAPPTLNLDNPSVETAIDLAPHKA
                     KPRKIDVALSNSFGFGGTNASLVLRKI"
     gene            complement(6396..6638)
                     /locus_tag="VKU03_09725"
     CDS             complement(6396..6638)
                     /locus_tag="VKU03_09725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HLJ71585.1"
                     /translation="MSDVAERVKKIVVEHLGVDADKVVENANFIDDLGADSLDTVELV
                     MAFEEEFGVEIPDDQAETIVTVGDAIKFLEKAAAAK"
     gene            complement(6951..7688)
                     /gene="fabG"
                     /locus_tag="VKU03_09730"
     CDS             complement(6951..7688)
                     /gene="fabG"
                     /locus_tag="VKU03_09730"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HLJ71586.1"
                     /translation="MFDLHGKGALVTGASGGLGGAIAKALHGQGARVALSGTRRDALE
                     KLAGELGEGAFVLPADLSDAASVEALPAAAEAALGAIDILVNNAGVTRDNLFLRMKDE
                     EWDQVLAVNLTAAFRLARGCLKGMMRRRYGRIIGISSVVGATGNPGQGNYAAAKAGMV
                     GMSKALAYEVASRNITVNCVAPGFIQSAMTDALNDKQREQILARVPMGRLGGGAEIAA
                     AVVYLASAEAAYVTGQTLHINGGMAMI"
     gene            complement(7703..8662)
                     /gene="fabD"
                     /locus_tag="VKU03_09735"
     CDS             complement(7703..8662)
                     /gene="fabD"
                     /locus_tag="VKU03_09735"
                     /EC_number="2.3.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006610959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004314 - [acyl-carrier-protein]
                     S-malonyltransferase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ACP S-malonyltransferase"
                     /protein_id="HLJ71587.1"
                     /translation="MAGTAFIFPGQGSQAVGMGKALAERYPAAREIFERVDAALGERL
                     STLMFDGPESDLTLTANAQPALMAVSLAVVRVLESQAGLDLGRDAEFLAGHSLGEYSA
                     LAAAGAFTVEDAARLLRIRGQAMQSAVPVGQGAMAAILGLDFAQSAAVAAEAAETPGA
                     VCQAANDNGGGQVVISGTKAAVERAMALAKLKGAKRAVPLPVSAPFHCALMQPAADAM
                     AAALATIEIKKPKAPIVANVTARPMREPGEIRENLVAQVVGAVRWRESVAYMAEHGVQ
                     RFVELGAGKVLSGLVRRIAEGATAINISGPAEVDAFRPAAVSS"
     gene            9425..10957
                     /locus_tag="VKU03_09740"
     CDS             9425..10957
                     /locus_tag="VKU03_09740"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE/TadG family type IV pilus assembly protein"
                     /protein_id="HLJ71588.1"
                     /translation="MRFDMFRRFAQDRRGNVAMYFGLAAVPLIFAVGMAVDYGNNARK
                     WSELNAAADAAALAAVTPAMMASSDATAQAAATNIFTAQANTISNIVPGTMVANVTIT
                     DSGLTRKVDVSYTAQVYNIFGGVLGQKTMPVSGHSQASGATPPNVDFYLLLDTSPSMA
                     IPATLNGINKMIANTQQQSDGNMKGCAFACHESNPTSSDSAGNPNGEDNYALAKSLTD
                     PDTGATGITLRIDEVANAATAMVSSAISTMNQNQTNFGWTPNYRIAVNTFDLGIHSLY
                     PITTTLSTLQSNLAANPSPIQLMEVYSNNNICTNWKTTTKNGVTTTTCTAYTNNNDTD
                     TDIDSTLTALNTSGNANYIPNPGTGATGSQPAEVLMIVTDGMEDACVSSITSGTTVSC
                     SGGKRQEAPMAASSATTSSSTTPSQVPSVATCAAIKARGIKIAVLYTVYYPLDSGSQN
                     TWYDNHIYEFQPNGVDQIGPSLQQNCASPGLFYQVNVGEDISKALVTLFQMALQSSHL
                     TQ"
BASE COUNT         1904 a         3769 c         3690 g         1947 t
ORIGIN      
        1 ggttggccga gccccgcctg acttcctgcg cctctatgac ctcaagcttg ggccggcgcc
       61 gcttctggtg atttttctcg cggcgctgac tctggccggg atttttgtgc tgcgacggac
      121 cgcgtttggc cgctacatct acgccatcgg cggcaacgaa caggtagcgc tgttgtccgg
      181 catccgcgtc gatcgggtca agatcggcgc ctatatgttg tgcagcactc tggccgcgct
      241 gaccggcctg ctctacttgg cccgcgccgg cattggcgat ccggtcacgg gcgaaggcgc
      301 ggagcttcag gcgattacgg ccgtgatcct cggggggacg agtttgtttg gcggtcgggg
      361 cggccttgtc ggcacgcttg gcggcgtatt gctcatgggc ctgaccaaca acgttctggt
      421 catcctcaac gtcagctcat ggtatcagga attgattcag gggctggtca tcgtcggcgc
      481 ggtcgcgctc tacaagcaaa agggacggta aatcgggccc gaattctgga gccagaagca
      541 accctgttct gcggccgcgg accggcggcg gcagcgcggc aagaactgct tgagaagctg
      601 aatggagtcc caggagccga tacaatcgag aaacaaagct tcttccgcct agccgcgttg
      661 agcggcgatc aaggcaaggc ttttagggca ataatatagg gcgaagaaag aagtaggacc
      721 gccatcgcaa ttgctgtgtt cagacctcaa taacgttgac ggaagttgat ggcgccggcc
      781 aggcctgtcc tacgtcacaa aaccggccag cgtttcgacg aacagccgcg aaaatctcgc
      841 cccatcgacg tcgacgcaca catccgcgtt gaatcgctcg gcggcggcgt tgcgccgccg
      901 gtcgacgatg acgcggccgc gggtaaacgc gccgtagagc tcgatatcga cgcacatccg
      961 ttcgaccgtc gccaggcttt tgtcctcggc aatggccacg gcgagcgggt catggaggcc
     1021 gcagccggcg attccagggt gcatgctctc ataagcacga acgtagaaat cggcgatttg
     1081 catcatcgtc gcgccgacct tgccgctctc ggcaatactc ttgcgcatcg cggcggtgag
     1141 cagcgccttc atcgtgacgt ccaggccaac cagagtgatc ttggcgcccg agcgcatcac
     1201 gatttgcgcc gcttccggat cgttccagac attggcgctg gcggtcggcg tcgggacgcc
     1261 ttggatgcca ggatggcgaa tggtcccgcc catgatcacg atttgcttga acagccgcgc
     1321 gacttcggga gcctttgcga gcgcgatcgc gacattggtg accggaccga ccgggcagag
     1381 cgtgatctcg ctgggatttt cgcgcgccag gcgaatcatc aggtcgctgg cgtgctcgcc
     1441 gaccggcttg gccttaggct cgggcagcgc cagatcgccg aggccgttgc ggccatgaac
     1501 gtgctcggcc cccttggcga acggtttcat gagcgagcga cccggcccgc gcgcgacagg
     1561 aatgtcgctg cggccggcca gttccaggat gcgtagcgtg ttgccggtgg cgctgtcgat
     1621 gtcggtgttg ccgaaagtcg tggtcaacgc ttcgagcttt atgcccggcc ggttcaacgc
     1681 atagaggatg gccatggcgt cgtcgacgcc ggtatcgacg tcgaggatca tgcggcagat
     1741 gtcggacatg gtggattgac cctcgcggcc gcggcgctgt cgatagcgaa ttaacttgtc
     1801 cggtttggta gcacatcatc tgtccgcttc ttctgctttt gttctcctgt gggcgttgtc
     1861 cacatttcca caggcgcgcc gccgagcgga tttaggcggc gtttttgtct tactcgaaat
     1921 ggaagcatca gccggcgacg cctccgcacc gagcgcgatg cccagcggga gcctcatcga
     1981 aaactgcagg ggacgcctga aaggcgaaat agccgggcgc caatcagaga cttagtggtg
     2041 ggggaggtag gactcgaacc tacgaaggca tagccagcgg atttacagtc cgcccccttt
     2101 gccgctcggg acactccccc gtcgggcgct cttcaacgag cagccgacga caaaggcccc
     2161 ggcgaaccgg ggcccagccc ggcgcttatc ttgcgccgcg gcaggtcctg tcaactcaaa
     2221 aagcgcgagc cggaaggcgc tgcggcggcg cgattgcgcc tggctcgggc gcgtggcagg
     2281 agaggctgcg gcgaggcgag ggaagagctg tttgtcgaag aagaacaaag gccgggcgag
     2341 accgggcgag cgcccgccgc cacgcttcgc gccgcgcgcc gagacgccca tacccgcgcc
     2401 gccccggcgc gggcggcaag aacggccggg cgcgccgcca ggcggccgcg acaggcgccc
     2461 gccgaacgat gtcgtgcaaa tctacggttt tcacagcgtc gaggcggcgc tcaaagcgcc
     2521 gcgacggagg ctgatccggc tttacgcgac gcccgccgcg gcggcgcggc tagccgccga
     2581 agcggcggcg cgcggcgtcg agacggtggt gatgagcaac gaagagatcg cgctgcgcgc
     2641 gccgcgccag gcggcgcatc agggcgtgct gctcgaagcg cggccgctcg cgcccatcga
     2701 tcttgccgaa atccccgagc gcggcctggt cgtggttctc gaccaggtca ccgatccgca
     2761 caatgtcggc gcgattttgc gcaccggcgc ggctttcgcc atcgacgcct tggtgacgac
     2821 cgagcgccat tcgccggagc tgaccggagc gctcgccaaa tcggcgtcgg gcggcctcga
     2881 acacgtgccg gtatgcagcg tcgtcaatct cgcccgcgcg cttcaagagc tcggcgatcg
     2941 cggctattgg cgcgtcgggc tcgattcgga gggaccgcaa tccctgtcgg acatggcctt
     3001 gtcgcggccg ctggccctcg tcctcggcgc cgaggacaag ggcctgcgcc ggctcacccg
     3061 cgaaaattgc gacgcgctgg cgaggctcga catgccgggc ccgatcaaga gcctcaacgt
     3121 ctcgaatgct tgcgcggtcg cgctgaccat cgcgcgcgcc cagctcggcg aagcaacgta
     3181 aggtacgtgt cattcccgct tgcgggaatg accgcggtca ctgcagccgc agcggcggca
     3241 cgaccttcgg atagttgaga tcgtaagtcg tattcagcag cggatcgagc gcggtgcctt
     3301 ccgaagcgtc gatgatccgt ggccgcccat tcgcggcagg ttttgacagc agcgccgagt
     3361 tcgtctcggc gttcgccatc ttgcccggcg gcggcgcggc ctcgccgtag cgcgcttcgc
     3421 ggctccgctg ctcggcgagc atttgcggcg agagcggata ggtctgcgcc ggagcagccg
     3481 cggtcgggtc ctccggcgtc gcgtcgttca ccccctcgac gacgaggccg ctatcggcga
     3541 ggttcttgcc cgtggcgacg gcggagagcg gcccgttcgg ccccagcaag gcgagccgcg
     3601 actgccgctc ggcgccgatc ctggcgagcc gcgacgccaa ggaggccgga gccggtttcg
     3661 tcgtgatgcg cggaacgagc gcgccgaaaa ccttggggct gacgtcgagc tcgctcttct
     3721 tcgggcgcgg gccgggctgc gccgaagcgg gcatgatcgg cagcaggtcg ggcgcgaccc
     3781 gatccttcgc ctctttcgca tcccgctcga tctgccgcca gtggtcgaca ttcttcaaat
     3841 gctggtcgag cgtctccgcg aaagcatggc cgccggtccc gtcggcgacg aaatagagat
     3901 cgttcgtgtc ggccggattg gcggccgcct ccaaggcggc tttgccagga ttggcgatcg
     3961 gtcccggcgg caggccctcg atgacatagg tattgtaggg cgtcggctga tcgagctcgg
     4021 atttggtgat cggatggtcg agcgtgccct tgccgccgac caggccgtaa acgatggtgg
     4081 ggtcggattg cagtttcatg cgtttcttaa gccggtggat gaagaccgcc gcgacgcgcg
     4141 gccgctcgtc ggccttgccc gtctctttct cgaccagcga agcaagcgtg acgagctcgc
     4201 caggcgattt gatcggcagg ttggccgccc gggcctgcca gatctcgtcg accactttgg
     4261 cctgcgcctt ctccatgcgc gccagcagcg cttcgcggct ttcgccgcgc gtaaagaaat
     4321 acgtatccgg caggatcgag ccttcgcgcg gcgtctcccg gatatcgccg accaacacgt
     4381 cgtcggcgcg cagcctttgc accacttgct ggctggtgag cccttccgga atggtcattt
     4441 tgtgctggac gggtttgccg gagaccaacg tgttctcgac ctcgcgcaag ctggcgcgcg
     4501 ccttgaatag atattcgccg cgcttcagct tgccgcgatt gccgtcggcg aggacgacca
     4561 cattgaacca cagcgtgctg ttgatcacgc cggctttttc aagctgatcg gcgatggtcg
     4621 cgccgtccgc ctcgtcttcg cgcgacagga tcacgacctt gtccgccggg agcgggccgg
     4681 gcctgcgcgc ttcctgcagc gcccaggcga agacgccgat gccgacgatt gcggcgatca
     4741 gcaggaaaga gagaacgccg ctgagccgcg acaggccttc acgccgctct ttgcgcggcg
     4801 gctcgggcgg cggcggcgcg gcttgcggct gcaaagcctc ggtcggcgaa cgcggcgtcg
     4861 cccgccggcc gaagagactg acgcgcggcc tgtcgccgcc cgagccgtcg ttgatcgatc
     4921 cgtcgcccat caagcttccg ccacgccgcc gcggacggcg ccccgaaata cgattctacc
     4981 cttaagcgcc aatatggcga aatcgggatt ccgcgaaagc ctcagatttt tcgcagcacc
     5041 agcgaggcgt tggtgccgcc gaacccgaac gagttggaca acgctacgtc gatcttgcgc
     5101 ggcttggcct tgtggggcgc aagatcaatc gcggtctcga cggaaggatt gtcgagattg
     5161 agcgtcggcg gcgcgatccc atcgcgcatc gcgaggatgc agaaaatcgc ctccaccgcg
     5221 ccggccgcgc cgagcaggtg gccgatcgag gatttggttg aggacatggt cagcgcgccc
     5281 gccgcattgc ccatcagccg ctccacggct ctgagctcga tcgcgtcgcc cggcggcgtc
     5341 gaggtgccat gggcgttgac gtagtcgatg tcggcgggcg ccagattggc gcgcttcagc
     5401 gccgccgtca tggcccggaa cgcgccgtcg ccgtcttcgg ccggcgccgt gatgtgatgg
     5461 gcgtcgccgg aaaggccgta gccgacgagc tcggcataga tgcgcgcgcc gcgggctcgg
     5521 gcatgctcca gctcttcgag gaccacgcaa ccggcgcctt cgcccatgac aaacccttcg
     5581 cggtcgcggt cataaggcct ggaggccttg gtcgggcggt cattgaagct ggtggcgagc
     5641 gcccggcagg cggcgaagcc ggctatgccc agccgattga ccgccgattc ggcgccgccg
     5701 gccaccatca cattggcgtc gccgagcgcg atcagccggc cggcgtcgcc gatcgcatgc
     5761 gcgccggtcg agcaggcggt gacgacggaa tgattcggcc ccttgagctt atgagcgatc
     5821 gagacatagc cgctcgtcag gttgatcagc cgcccggtga tgaaaaaggg cgacatcttg
     5881 cgggggccgc gctctttcaa gaccatcgag gcttcgtaga tgccgccgag gccgccgatg
     5941 ccggagccga tcatgacgcc ggtctcgatc tcctcttcgt agcttgaagg ggcccaattg
     6001 gcgtctttca gcgcctgcgt cgcggccgcc atcgaataga caatgaagtc gtcgacccgg
     6061 cggtgctccc gcggctccat ccaatcatcg ggattgaagc gatcctcgct gagaggcccg
     6121 cgcggcactt gcgcggcgat ttgacagggc agatcggaga cgtcgaaatt ctcgacgcgg
     6181 ccgacgccgc tttctccggc gagcagccgg cgccaggtgg cctcgacgcc gcaagccagc
     6241 ggcgtgacca tgccgagtcc tgtgaccacc acgcgcttca tcaaaaatcc gcctccagcg
     6301 cgtcctcctg acgcgacaag ggccggtcgc agccggcccc cgagattcac agcgcggcct
     6361 cgcccgccgt caaggcggga ggcggacttt ttaacctatt tggccgccgc ggccttctcc
     6421 aggaatttga tcgcgtcgcc gaccgtgacg atcgtctcgg cctgatcgtc ggggatttcg
     6481 acgccgaact cctcttcgaa cgccatcacc aattccaccg tatcgagcga atcggcgcca
     6541 aggtcgtcaa tgaaattggc gttctccacc accttgtcgg cgtcgacgcc aagatgctcg
     6601 acaacgatct tttttacgcg ctcagcgaca tcgctcattg tgtctgtcct taagactttg
     6661 cggcgtcatc aaacgcccgc ttttgttgga tcccctaacc tgaccgcctg ctttcgccgt
     6721 ctcgcggcct taaaaagcgc gattcggcgc gttcccatca tccggcgtcg gcgcgtctcg
     6781 gcttgaaggg gccttcacgc gccggccgac gctcaagcaa tgatcaggtc aaatttccgc
     6841 ccgcattttc ggccgtttgg cggcccggcg ctccgatagc atatctcggt aagcaaggcg
     6901 agagccccgc aacggcgctg agcagcgcgc ccgcgagcct ggcaagcccc tcaaatcatt
     6961 gccattcctc cgttaatatg gagcgtctgg ccggtcacgt aggcggcttc cgcgctggcg
     7021 agatagacga cggccgccgc gatttcggcg ccgccgccga gccgccccat tggcacgcgg
     7081 gcgaggatct gctcgcgctg cttgtcgttc agcgcgtccg tcatggcgct ctggatgaag
     7141 cccggcgcga cgcaattgac ggtgatgttg cggctggcga cttcgtaggc caaagccttg
     7201 gacatgccga ccatcccggc tttcgccgcc gcgtaattgc cctggccggg attgccggtc
     7261 gcgccgacga ccgagctgat cccgatgatc cggccgtagc ggcggcgcat catgcctttc
     7321 aggcagccgc gcgccaggcg gaaggcggcg gtgaggttga cggcgaggac ttgatcccac
     7381 tcctcgtcct tcatcctcag aaaaagattg tcgcgcgtga cgccggcatt gttcaccagg
     7441 atgtcgattg cgccgagggc ggcctcggcc gccgccggca gcgcttcgac cgaagccgcg
     7501 tcgctgagat cggccggcag gacgaaggcg ccctcgccga gctcccccgc cagtttctcc
     7561 agcgcgtcgc gcctggtgcc ggacagcgcg acccgcgcgc cctggccatg cagcgccttg
     7621 gcgatcgcgc cgccgagccc gcccgaagcg ccggtgacga gcgcgccctt gccgtgaaga
     7681 tcgaacatgc tttgtcctgc gatcaggaag aaacggccgc cgggcggaag gcgtcgacct
     7741 ccgccggccc gctaatattg atcgccgtcg cgccctcggc gatgcgccgg acgaggccgc
     7801 ttaagacctt gcccgcgcca agctcgacaa agcgctgaac gccgtgctcg gccatgtaag
     7861 caacgctctc gcgccagcgc accgcgccga cgacttgcgc gacgaggttc tcgcgaatct
     7921 cgcccggctc gcgcatcggc cgcgccgtga cattggcgac gatcggcgct ttcggcttct
     7981 tgatctcgat cgtcgcgagc gccgccgcca tggcgtcggc ggccggctgc atcagcgcgc
     8041 aatggaaggg ggccgagacc ggcagcggca cggcgcgctt cgcgcccttc agcttggcga
     8101 gcgccatcgc ccgctcgacc gccgccttgg tcccggaaat gaccacctgg ccgccgccat
     8161 tgtcgttggc cgcctggcag accgcgccgg gcgtttccgc cgcctcggcc gccacagccg
     8221 cgctctgcgc aaaatcgagg ccgagaatcg ccgccatcgc gccctggccg acggggaccg
     8281 cgctctgcat cgcttggccg cggatgcgca gcagccgcgc cgcgtcctcg acggtgaagg
     8341 cgccggcggc ggcgagcgcc gaatattcgc cgagcgaatg gccggccagg aattccgcgt
     8401 cgcggccgag atcgaggccg gcttggcttt ccagcacgcg gacgaccgcg aggctgaccg
     8461 ccatcagggc gggctgggcg ttggcggtga gggtgagatc ggattcgggg ccgtcaaaca
     8521 tcagcgtcga aaggcgctcg cccaaggcgg cgtcgacgcg ctcgaaaatc tcgcgcgccg
     8581 ccggatagcg ctcggccagc gccttgccca tgccgaccgc ctgcgatcct tgtcctggaa
     8641 agatgaaagc cgttcccgcc atcgccaagc cttaattttg gcgggcagag acattgcttg
     8701 gccgagcctg tcaagctggg cggctcttat cctcccccgc aaaagcgggg gaggggaccg
     8761 cgcgtcagcg cggtggaggg ggccgccggc ggttgggatt atcctgatgg accgcggccc
     8821 cctccaccat gcttcgcatg gtccccctcc cccgttccgc ttcgctgcac gggggaggag
     8881 aagagcgcgt taggctgcgg agagccgcgc gtggaatttg ctgccggtcg ccgccgatcc
     8941 ttccccgcga aagcggggga gggggaccgc gcgtcagcgc ggtggagggg gccgccgggg
     9001 gttgggatta tcctgataga gcgccgcccc ctccaccatg cttcgcatgg tccccctccc
     9061 ccgtttcgct tcgctgcacg ggggaggaga agagcgcgtc aggctgcgga gagccgcacg
     9121 tggaatttgc tgccggccgc cgccgatcct cccccgcaaa agcgggggag ggggttagcg
     9181 cggtggaggg ggccgccggc ggttgggatt atcctgatgg accgcaggcc ccctccacca
     9241 tgcttcgcat ggttcccctc ccccgtttcg cttcgctcca cgggggagga gggaaagtcc
     9301 cgaagattcg cgcattcgcg taacaaatcc ttagagcggg ccgggccata atcccggccg
     9361 gtctgtactg gacgagccgg cgcggccgct tgtccctgcg acagaagccg tctgagagga
     9421 cgccatgcgt ttcgatatgt tccgccgctt cgcccaggac cgccgcggca atgtcgcgat
     9481 gtatttcggc ctggcggccg tcccgctgat tttcgccgtc ggcatggcgg tcgattacgg
     9541 caacaacgcc cgcaaatggt cggagctcaa cgccgccgcc gacgccgccg cgctcgccgc
     9601 tgtgacgccg gcgatgatgg cctcctccga tgcgacggcc caggccgccg cgaccaacat
     9661 cttcacggcg caagccaaca cgatttcgaa catcgtcccc ggcaccatgg tggcgaacgt
     9721 caccatcacc gacagcggcc tgacgcgaaa ggtcgacgtc tcctataccg cccaggtcta
     9781 caacatattc ggcggcgtgc tcggccagaa gacgatgccc gtcagcggcc attcccaggc
     9841 cagcggggcg accccgccca acgtcgattt ctacctcctg ctcgacacct cgccgtcgat
     9901 ggccattcca gcgactctga acggcatcaa taaaatgatc gcgaatactc agcagcaaag
     9961 cgacggcaat atgaagggct gcgcattcgc ctgccacgag agcaatccga catccagcga
    10021 tagcgccggc aatccgaacg gcgaggacaa ttacgcgctc gccaaatcat tgaccgatcc
    10081 ggataccggc gcaaccggca tcacgctgcg catcgacgag gtcgccaacg ccgcgaccgc
    10141 gatggtgagc tcggcgatca gtacaatgaa ccaaaatcag accaatttcg gatggacgcc
    10201 gaactaccgg atcgctgtca acacgttcga tctcggcatt cattccttgt accccatcac
    10261 gacgaccctt tccacgctgc aatccaatct cgccgcgaac ccgagcccga tccagttgat
    10321 ggaggtctat agcaacaaca atatctgcac caattggaaa acgaccacca aaaacggtgt
    10381 gacgacgacg acctgcacgg cctacacgaa caacaacgac accgatacgg atatcgattc
    10441 gactttgaca gcgttgaaca cgtcaggcaa tgccaactac attcccaatc ccggcaccgg
    10501 cgcgaccggc agtcagcccg ccgaagtgct gatgatcgtt accgacggca tggaggacgc
    10561 gtgcgtcagc tcgatcacct ccggcacgac ggtgtcatgc tcgggtggga agcgccagga
    10621 agcgccgatg gcggcgagct ccgccacgac aagctcaagc acgacgccgt cgcaggtgcc
    10681 ttccgtcgct acatgcgccg cgatcaaggc gcgcggcatc aagatcgcag ttctctacac
    10741 cgtctattat ccgctcgaca gcggcagcca gaacacttgg tacgacaatc acatctacga
    10801 gttccagccg aacggcgtgg accagatcgg cccatcgctt cagcaaaact gcgcctcgcc
    10861 cggcctgttc taccaggtga atgtcggcga ggacatttcc aaggcgctgg tgaccctgtt
    10921 ccagatggcg ctgcagagct cgcacctcac ccagtaacac gcaagatctg gcggggcgcg
    10981 tctgtaggct aacgcgcctc cgctcgtcat ggccgggctt gtccccggcc atcgacgcgc
    11041 cgccgtcctc gccgcgaggc ggcgcctcat ggccgggctt gataggacgt cattgcgagg
    11101 agagcagcga cgaagcaatc cagtcgcagg gcggcgctct acgagtggag cgcgtcgcgt
    11161 cgcacgcggc gctctgtcat ttgggcgtaa gcgggaatcc aggccgccac ccttcaggcg
    11221 cggcggcttt ctggattccc gctttcgcgg gaatggcgtc acggcgtggt cgtgggttgg
    11281 tcgatgagga cgccggtcga caggcgcgga
//
LOCUS       DATGJW010000318         7103 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_373932, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000318 DATGJW010000000
VERSION     DATGJW010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7103)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7103)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7103
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_373932"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1464
                     /gene="tssI"
                     /locus_tag="VKU03_09745"
     CDS             <1..1464
                     /gene="tssI"
                     /locus_tag="VKU03_09745"
                     /inference="COORDINATES: protein motif:HMM:TIGR03361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system tip protein TssI/VgrG"
                     /protein_id="HLJ71589.1"
                     /translation="EYCVQYRETDFDFVSRLMEQYGIYYFFEYEDGKHTLVLADQKAS
                     HKAIPDLQTVSFVPVAEVGRREKQHIEEWSRGRAVESGCFKLNDYDYNKPSANLLANS
                     DKPGGYAHDSMEMYDYIGEYSDRSVGDQLAEVEAQAAQSLDDRRTGTGSALSLFPGGL
                     VNLKDHPVGAENQEYLVVACSHFFHAQQYRSGGVSDGPAYTGNFEFTPSSRQFRAELD
                     TERPQISGYQSALVVGKQGEEIDVDEKGRITVQFYWDRKKKPSRRVRVAQFWAGKDRG
                     AWFVPRIGDEVLIHYEEGDPDRPIVVGSVYNGDNKLSLNMPADKNKSGLLTKSTKNSD
                     GYNFLLFNDTSGSEVVKLRCQRDLMFKALRNEQRDIGGDQTENIGGDETINVGGPTGG
                     GHFEVNAVQAITLNVGPMGGPITQIHMDETGIFMSAGMGISTMQITPEGITLNAPLID
                     ASADAAIDLDAPAIVAGETLTTPELNAGAATVSGIPI"
     gene            1472..2059
                     /locus_tag="VKU03_09750"
     CDS             1472..2059
                     /locus_tag="VKU03_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71590.1"
                     /translation="MSSRIRFATARAVFEAFPDLRRLAPPPADDLPALDYARRLLALR
                     KKSALAYLAFLLPRREAVWWALQCVAALSPRDAEDAALQAAEAWVRAPEEERRRAALD
                     IGQTADRRRPSTWLAWAAARSGGSMTAPDQKPQPAQPSSCARAANAAVILAACAGDAQ
                     AIPQRLQACAEAGIRFAEGGDARPTIAARPGGKGR"
     gene            complement(2200..2484)
                     /locus_tag="VKU03_09755"
     CDS             complement(2200..2484)
                     /locus_tag="VKU03_09755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HLJ71591.1"
                     /translation="MFRLLGSDGVIYESNQPGALGGHRKLKIYGSLDCESAKRAIAKG
                     QYVKQRVFFADETTAIAAGYRPCQKCLPKRHAIWKADPDAWPLNASRMDW"
     gene            complement(2679..3476)
                     /locus_tag="VKU03_09760"
     CDS             complement(2679..3476)
                     /locus_tag="VKU03_09760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HLJ71592.1"
                     /translation="MSDRLVFETASASHVGLVRTVNEDSLLARPDIGLWAVADGMGGH
                     GGGDVASKAVIDALDTIKRADSAAELLAQFERRIIRVNAELRAFARSRQFGAIGATLA
                     AILVRPPHYACLWCGDSRVYLWRGGAMNRISHDHSEVQDLIDRGLLDPAEAKTWPRRN
                     VVTRAVGVAVEAEIELGEGRVAAGDRFLLCSDGLTNHVEDAEIAAALGGDPNKACAAL
                     LDLTLQRGASDNVSLIVVACESDARTIRAEASWRAEAALAGQRPPRA"
     gene            complement(3487..4182)
                     /gene="tagF"
                     /locus_tag="VKU03_09765"
     CDS             complement(3487..4182)
                     /gene="tagF"
                     /locus_tag="VKU03_09765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system-associated protein
                     TagF"
                     /protein_id="HLJ71593.1"
                     /translation="MTAGLYGKLPAKRDFVAFNTPRAFLGVWEPWLQAGVAISRQILG
                     ESWRDFYLSAPIWRFWLGADFCGESVLGAFMPSIDGVGRYFPLTIFAGESAGALPPPE
                     IEPNKEWFDAVETILLDALQPEQDFDRLAAAVAALAAPIQTPPDTAVAGIALLSDGAI
                     LVRDFGDELAVALRAARRFGHRRAFAGQSCWWTIGGAGFQPAALVETGLPRPERFAEM
                     LTGSFDRSLAVPA"
     gene            complement(4190..>7103)
                     /gene="tssM"
                     /locus_tag="VKU03_09770"
     CDS             complement(4190..>7103)
                     /gene="tssM"
                     /locus_tag="VKU03_09770"
                     /inference="COORDINATES: protein motif:HMM:TIGR03348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system membrane subunit TssM"
                     /protein_id="HLJ71594.1"
                     /translation="DDIVRLPPAEVNAHADAIRKRLAELHDELKIDFPVYAIFTKMDL
                     VVGFTQYFADLDEAKRRAVWGATFPGADRKANQIGALPGEIDLLVQRLIERMPERLQD
                     EPDLRARALLFGLPAQISALKKPIVDFLNRIFEPTRYQANAILRGFYFTSGTQEGTPF
                     DAVIGALQKSYGVESFGAAAFSGAGKSFFLHDLLTKVIFGEAGWVSTNMAAVRRSFAF
                     RAAAFSLIALATIAVLAAWYVSYSRNSALIAQTSRAFDDYAAAAGPLLKQTSVGDPDL
                     RPVYERIGALLNLPVGYANRDAPTPIPQTFGLSERDRLQAASDSAYQAALERLMRPRL
                     VLRLEQQIQKNVNDPAFVYEALKVYLMLGGKAPHIDKDLIVDWFARDWEESMYPGAPY
                     RQGRDLLRRHLVAMLDLDNGQAPKISLNGPLVEQAQATLARMRVAERAYTLLKSEAHN
                     ERIEDWVASQRGGPDVGLVFEAANGASLDTVRVPGFFTYAGFYHALLDHMPTIAEKLE
                     KEQWVLGQSGDQSAVKQQYASLMPDILELYGKDFVAAWNVALSNLQLRPMLADKPKYL
                     ALSAASAATSPIKQIFESVRDETALTKERRASQPANADQKKATEMQAAQMAASRLGST
                     ASQAVALAMKSQRRAGDPPAETPGASIEANFKPFQVLFEGEPGSRPMDALIANLNELD
                     RDLLLAAENPTQAKQALAQVQLQVASLRSNVSRLPQPLSGMMQNVANDIDGDTAATSI
                     AQLSQALAEQVTGPCQQIIANRYPFAKSERDVPMADFARLFAPNGLIDKFFSSSLAPL
                     VNQTGKTWAWLPNPHLTRKLSETTLRQFQQAAEIRDTFFPTGGSQPTVSLEVKPLTLS
                     GEAQSATLQINGASVVAQQGTNAPSTVQWPGAGAGAASLVLAPDLPDRISKLERSGAW
                     ALFRLVDAGSALQRGNAVSASFVVGGREASFQFTAASLQNPLSLPALRQFKCPNGL"
BASE COUNT         1276 a         2284 c         2351 g         1192 t
ORIGIN      
        1 gaatattgcg tgcaataccg cgagaccgat ttcgatttcg tctcccgcct gatggagcaa
       61 tacggcatct actatttctt cgaatatgaa gacggcaagc acacgctggt tctcgccgac
      121 cagaaggcca gccacaaggc gattcccgac ctgcagaccg tctcctttgt gcccgtcgcg
      181 gaagtggggc ggcgggaaaa gcagcatatc gaagaatggt cgcgcgggcg cgcggtcgag
      241 agcggctgct tcaagctcaa cgattacgac tacaacaagc caagcgccaa tcttctcgcc
      301 aattccgaca agcccggcgg ctatgcgcac gattccatgg aaatgtacga ttatatcggc
      361 gaatattccg atcgcagcgt cggcgatcag ctcgccgagg tcgaggcgca ggcggcgcaa
      421 tcgttggacg accgccgcac cggcaccggc tcggcgctgt cgctttttcc gggcgggctg
      481 gtgaacctga aggatcatcc ggtcggcgcc gagaaccagg aatatctcgt cgtcgcctgt
      541 tcgcatttct ttcacgccca gcaatatcgc tccggcggcg tttccgacgg gccggcctat
      601 accggcaatt tcgagttcac gccgagcagc cggcagttcc gcgccgagct cgacaccgag
      661 cggccgcaga tttccggcta tcagtccgcg ctcgtcgtcg gcaagcaggg cgaagagatc
      721 gatgtcgacg aaaagggccg gatcaccgtc cagttctatt gggaccgcaa gaagaaaccg
      781 tcgcgccgag tgcgcgtcgc gcaattctgg gctggcaagg atcgcggcgc ctggttcgtt
      841 ccgcgcatcg gcgacgaagt gctcatccat tacgaggaag gcgatcccga ccgcccgatc
      901 gtcgtcggct cggtctataa tggcgacaac aagctctcgc tgaacatgcc ggccgataag
      961 aacaaatctg gccttctgac caaatcgacc aagaacagcg acggctacaa tttcctgctg
     1021 ttcaacgaca cgtcgggcag cgaggtcgtc aagctgcgct gccagcgcga cctgatgttc
     1081 aaggcgctgc gcaatgagca gcgcgacatc ggcggcgacc agacggagaa tatcggcggc
     1141 gacgagacga tcaatgtcgg cggcccgacc ggcggcggac atttcgaggt caacgcggtc
     1201 caggcgatca cgctcaatgt cgggccgatg ggcggcccga tcacgcaaat ccatatggac
     1261 gagaccggca tcttcatgag cgccgggatg ggcatctcga cgatgcagat cacgcccgag
     1321 ggaatcacgc tcaacgcgcc gctgatcgac gcgtccgccg acgcggcaat cgatctcgac
     1381 gcgcccgcca tcgtcgccgg cgagaccctg accacgcccg aactgaatgc cggcgcggct
     1441 acggtcagcg gcatcccaat ataggtcggc gatgtcgtcc cgcatccgat tcgcgacagc
     1501 gcgcgccgta ttcgaagcct ttccggacct gcggcggctg gcgccgccgc ccgccgacga
     1561 ccttcccgcg ctcgactatg caaggcggct tctcgccttg cgcaagaaga gtgcgctcgc
     1621 ctatctcgct ttcctgctgc cgcgccgcga agccgtctgg tgggcgctgc aatgcgtcgc
     1681 cgctctatcg ccgcgcgacg ccgaggacgc cgcgctgcaa gcggccgagg cttgggtgcg
     1741 cgcgccggaa gaagagcgcc gacgcgcggc gctcgatatc ggccaaaccg ccgacaggcg
     1801 tcgcccgtcg acgtggctcg cttgggccgc ggcccgttcc ggcggaagca tgaccgcgcc
     1861 cgaccagaag ccgcagccag cgcagccgtc atcttgcgcc agagcagcca atgccgcggt
     1921 catcctggcg gcttgcgcgg gcgacgcgca agccatcccg cagcgcttgc aagcctgtgc
     1981 cgaagccgga atccgcttcg ccgagggcgg cgacgcccgg ccgacgatcg ccgcgcggcc
     2041 aggcggcaag gggcgctgac ggctagtaat ctcccccgct tccattgcga gcgcacaaag
     2101 caatccattc gtagggcgga actctcgaat ggattttctt cgtcgcttca ctcctgcaag
     2161 aagccgagac cgaaagatgc cgcggcagag ggagagagat caccaatcca tccggcttgc
     2221 gttcagtggc caagcgtcgg gatcggcctt ccatattgcg tgtcgcttcg gaaggcattt
     2281 ctggcagggg cggtacccgg ccgcaatggc ggtcgtctcg tcggcgaaga acaccctttg
     2341 cttgacgtac tgccctttgg cgatcgcgcg tttggcgctt tcgcaatcaa ggctgccgta
     2401 tatcttcagc ttgcgatgac cacccagagc gccgggctgg ttgctctcat agatgacgcc
     2461 atcggagccg agcagtctga acatgccagt gcttttttga ttcgaaaccg actaaaacag
     2521 cacgacgcgt gacacttttc ttcagccgcg caggtacata tatcagcaac aataaggcgc
     2581 ggcgtcattg cgagcgaaac tgtccattcg tcgaggcggc gctctatgaa tggcttcgtc
     2641 gcttcgctcg tcgcaaacgg ctgcgattca agcgtccttt aagcacgcgg cggccgctgg
     2701 ccggccaaag cggcctcggc gcgccatgac gcttcggcgc ggatcgttcg cgcgtcgctc
     2761 tcgcaggcga cgacgatcag cgagacattg tcgctcgcgc cgcgctgcag cgtcaggtcg
     2821 agaagcgcgg cgcaggcctt gttcgggtcg ccgcccagag ccgcggcgat ctccgcgtcc
     2881 tcgacatgat tggtcagccc gtcgctgcac aggagaaagc ggtcgcccgc cgccacgcgc
     2941 ccttcgccga gctcgatctc cgcctccacg gcgacgccga cggcgcgggt gacgacgttg
     3001 cggcgcggcc aggtcttggc ctccgcggga tcgagcaggc cgcgatcgat caggtcctgc
     3061 acttcggaat ggtcgtgcga gattctgttc atggcgccgc cgcgccaaag gtacacccgg
     3121 ctgtcgccac accagaggca cgcgtaatgg ggcgggcgga cgagaatggc ggcgagcgtc
     3181 gcgccgatcg cgccgaattg ccgggagcgc gcgaaggcgc gtaactcggc gttgacgcgg
     3241 atgatccgac gctcgaattg cgccagcaat tccgccgcgc tgtcggcgcg cttgatcgta
     3301 tcgagcgcgt cgatcaccgc cttgctcgca acgtctccgc cgccatggcc gcccatgccg
     3361 tcggccaccg cccagaggcc gatatccggc cgcgccagca aactgtcctc gttgacggtt
     3421 ctgaccaggc cgacatggct ggcggaggca gtctcgaaga ccagacggtc ggacattgcg
     3481 cctctcctaa gccggaacgg cgagcgaacg atcgaatgaa cccgtcagca tctcggcgaa
     3541 gcgctccggt cgcggcaggc ccgtctcgac aagcgcggcc ggttgaaagc cagcgccgcc
     3601 gatcgtccac cagcaggact ggccggcgaa agcgcgacgg tggccgaagc gccgcgccgc
     3661 gcgcaacgcg acggcgagct cgtcgccgaa atcgcgcacc aggatcgcgc cgtccgaaag
     3721 cagcgcgatg ccggcgacgg cggtgtcggg cggcgtctga atcggcgcgg cgagcgccgc
     3781 gaccgcggct gccaagcggt cgaaatcctg ctcgggctgc agggcgtcga gcaggattgt
     3841 ctcaaccgcg tcgaaccatt ccttgttcgg ctcgatctcc ggcggcggca gggcgccggc
     3901 gctttcgccg gcgaaaatcg tcagggggaa atagcgcccg accccatcga tcgacggcat
     3961 gaaggcgccg agcacgctct cgccgcaaaa atcggcgccg agccagaagc gccagatcgg
     4021 cgcgctgaga tagaagtcgc gccagctttc cccgagaatc tggcgcgaga tcgcgacgcc
     4081 agcctgcagc cagggctccc acacgcccaa gaaggcgcgc ggcgtgttga acgcgacgaa
     4141 gtcgcgcttg gccgggagct tgccatagag gccggcggtc atccgcgcct cacaagccgt
     4201 tcggacattt gaactgccgc aacgcgggca ggctcagcgg attctggagc gaagcggcgg
     4261 tgaattgaaa cgatgcttcg cggccgccga cgacgaagct ggcgctcacc gcattgccgc
     4321 gctggagagc ggagcccgcg tcgaccaagc ggaacagagc ccaagcgccg ctgcgctcga
     4381 gcttggagat gcggtccggc agatcgggcg cgaggacgag cgacgccgcg ccggcgccgg
     4441 cgcccggcca ttgcaccgtc gacggcgcat tcgtaccctg ctgggcgacg acgctcgcgc
     4501 cgttgatctg cagcgtcgcg ctttgcgctt cgccgctcag cgtcaaaggc ttgacctcca
     4561 aactgacggt cggctggctg ccgccggtcg ggaagaacgt gtcgcggatc tcagccgcct
     4621 gctggaactg gcgcaaggtc gtctcggaca atttgcgcgt cagatgcggg ttgggcagcc
     4681 aagcccaggt cttgccggtc tggttgacga gcggcgccag gctcgaagag aagaacttgt
     4741 cgatgaggcc gttcggcgca aagagccggg cgaaatcggc catcggcacg tcgcgctcgc
     4801 tcttggcgaa cggatagcga ttggcgatga tctgctggca cgggccggtc acctgctcgg
     4861 cgagggcctg cgaaagctgc gcgatcgagg tcgcggcggt atcgccgtca atatcattgg
     4921 cgacgttctg catcatgccg gagagcggct gcggcagccg cgagacattg ctgcgcaagc
     4981 tcgcgacttg gagctgaact tgcgccaagg cttgtttggc ctgggtggga ttttccgcgg
     5041 cgagcagcag gtcgcgatca agctcgttga gattggcgat gagcgcgtcc atcggccgcg
     5101 agccgggttc gccctcgaac aggacctgga acggcttgaa attcgcttcg attgacgcac
     5161 cgggcgtttc ggccggcgga tcgcccgcgc gccgctgcga tttcatcgcc aaggcgacgg
     5221 cctggctggc ggtcgagccc agacgcgacg cggccatctg agcggcctgc atttccgtcg
     5281 ccttcttttg gtcggcgttc gctggttgcg acgcgcggcg ctctttggtg agagcggttt
     5341 cgtcgcgcac cgattcgaaa atctgcttga tcggcgaggt cgccgccgag gccgcgctca
     5401 aggcgagata tttcggcttg tcggcgagca tcggccgcaa ttgcagattg ctgagcgcga
     5461 cgttccaggc ggcgacgaag tccttgccgt aaagctcgag aatgtccggc atcaggctgg
     5521 cgtattgctg cttgaccgcg ctttgatcgc cggactggcc gaggacccat tgctccttct
     5581 ccagtttctc ggcgatggtc ggcatgtggt cgagcagagc gtggtagaag ccggcatagg
     5641 tgaagaagcc gggcacgcgc accgtatcga ggctcgcgcc gttggcggcc tcgaaaacca
     5701 ggccgacgtc ggggccgccg cgctgcgaag cgacccaatc ctcgatcctc tcgttatgcg
     5761 cctcggattt cagcagcgta taagcgcgct cggcgacgcg catgcgggcg agcgttgcct
     5821 gcgcctgctc gacgagcggc ccgttcagcg aaatcttcgg cgcctggcca ttgtcgagat
     5881 cgagcatggc gacaaggtgc cggcgcagca gatcgcgtcc ctggcgatag ggcgcgccgg
     5941 gatacatgct ctcctcccaa tcgcgcgcga accaatcgac gatgaggtcc ttgtcgatat
     6001 gcggcgcctt gccgccgagc atcagataga ctttcagcgc ttcgtagacg aaagccgggt
     6061 cgttgacgtt cttctggatc tgctgttcga gccgcagcac gaggcgcggc cgcatcagcc
     6121 gttcgagcgc ggcctgatag gccgaatccg acgcagcttg cagccggtcg cgctcgctga
     6181 gcccgaacgt ctgtgggatc ggggtcggcg cgtcgcgatt ggcgtagccg accggcaggt
     6241 tgagcaaagc gccgatgcgc tcgtaaaccg ggcgtagatc gggatcgccg accgaagttt
     6301 gcttgagaag cgggccggcc gcggcggcgt aatcgtcgaa cgcccggctc gtttgcgcga
     6361 tcagcgcgct gttgcggcta tagctgacgt accatgcggc gagaacggcg atcgtcgcga
     6421 gcgcgatcag tgaaaaggcg gcggcgcgga aggcgaagga gcggcggacc gccgccatat
     6481 tggtcgagac ccagccggcc tcgccgaata tcacctttgt caaaagatcg tgcaggaaga
     6541 agctcttgcc ggcgccggaa aaggccgccg cgccgaagct ctcgacgccg tagctctttt
     6601 gcagcgcgcc gatcaccgcg tcgaacggcg tcccctcctg cgtcccggac gtgaaataga
     6661 agccgcgcaa aatggcgttg gcctggtagc gggtcggctc gaaaatgcgg ttgaggaaat
     6721 cgacgatcgg ctttttcaag gcgctgatct gggccggcag gccgaaaagc agcgcccggg
     6781 cgcgaagatc cggctcgtcc tgcaagcgct cgggcatacg ctcgatcagc cgctgaacaa
     6841 gcagatcgat ctcgccgggc agcgcgccga tctgattggc tttgcggtcg gcgcccggga
     6901 aggtcgcgcc ccaaaccgcg cgacgcttcg cttcgtcgag atcggcgaaa tattgggtga
     6961 aaccgacgac gaggtccatc ttggtgaaaa tggcgtagac cgggaaatcg atcttcaatt
     7021 cgtcgtgcaa ttcggcgagg cgcttgcgga tcgcgtcggc atgggcgttt acttcggccg
     7081 gcggcaggcg gacgatgtcg tcg
//
LOCUS       DATGJW010000319         5105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1410124, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000319 DATGJW010000000
VERSION     DATGJW010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5105
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410124"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..889
                     /gene="pseI"
                     /locus_tag="VKU03_09775"
     CDS             <1..889
                     /gene="pseI"
                     /locus_tag="VKU03_09775"
                     /EC_number="2.5.1.97"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008136223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pseudaminic acid synthase"
                     /protein_id="HLJ71595.1"
                     /translation="ADTITIDVHSSDFTISDPDSLWAGRNLYDLYKEAHTPWEWHAPI
                     MVRAKARGLAAFSTPFDESAVDFLERLGVPAYKIASFENVHLPLIRKVAATGKPIILS
                     TGMASLAELDEAVAAAREAGCRDLVLLKCTSTYPATPENTNLATIPHLRELFGCEIGL
                     SDHTMGLGVAVAATALGASVIEKHFTLRRADGGVDSAFSLEPDELRALVVETGRAAAA
                     LGKVAYGPTEAEKKSLIFRRSLYLVRDVEEGEVLTRDNVRIIRPAGGLPPKAIDIVLG
                     MKARRKAPRGAPVTWDLVR"
     gene            924..2507
                     /locus_tag="VKU03_09780"
     CDS             924..2507
                     /locus_tag="VKU03_09780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71596.1"
                     /translation="MMRVGFASIYSWRPHVSMLHYLAGLARQAGHETYFLTCDGDLPN
                     CYTREQKPNRPAWLNCALCRAGGVRSYESRNVASIGALDGGDAAPAPRAREWVLSSAS
                     TLGRFESDADFAGAEFAGFADRLEPAARRAYSAARRWIDANRLDAIVAFNGRMDATRA
                     IFEAARDAQIRFVSQERSWFGDGVQLLPDENCLGLRAVDKMIAAWRDRPLTRGQALRA
                     ARHAAARFLRKNDKEWRAFNLEATKASWPSAGAERRILLLPGSRNETWGHPDWLDQWP
                     ERTAAFDAIIARLKLRPEEVLLRCHPIWTEAVSGRSGALPERYYRDWAAKAGVRAIPS
                     AARDSTMDLIRAADAIVVAGGSAALEAGLLGKQVIAASPSIYQRAGFQTTAYGPADLA
                     ELALITTLPPPALRAERRRIRRLTLRFCFAAAYRLPQFVPFVEAATTTRYNYYFGADP
                     WRLTRLLESGEVEADDPACADDEAAEDAVLDMIEREEWADIDAAAQNAPRGRTLKNDR
                     RGLYRAVDRARELMPRGDL"
     gene            2611..3552
                     /locus_tag="VKU03_09785"
     CDS             2611..3552
                     /locus_tag="VKU03_09785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71597.1"
                     /translation="MTRLASMVAAFRANPNYAMMRGAARFRSINGVVKGAKGLIEGRA
                     YRALLDDVKARLGQSRFSAVDPDAFVADLRRDGCAFGLTLPAADVEAIRAYAARETVY
                     ADRNSSLGFPLARRLEAEKALGKPILVAQYFNTEAQCPTIAKLRADPFLHYAAARYFG
                     SAPFSHDPNLWWTFATNASEEDKIKHAHRFHSDVDDFAILKFFFYITDVAVGDGSHVL
                     VRGSNRERPYRKFSDRLVLRRYMDDEITSFYPADRIVDIGGPAGTGFAEDTFCIHKGN
                     SPFDKPRLLLQFQFTLFDYDAQRDRIPAEQLKRLPLG"
     gene            3549..4859
                     /locus_tag="VKU03_09790"
     CDS             3549..4859
                     /locus_tag="VKU03_09790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71598.1"
                     /translation="MSAAPLRFRPIAPFIERALRFFSYTPLLGLSAVVSLAKLIVYAR
                     LVSADEYGAIAQMFLIGSTFGVVGSFGFYLLALRELPGLHVRRREFAMAILLGQAAII
                     STASAAAACVLLIPFGAAGVFATRIGGLGIAVGLFYAWTQLCFQIATTEARSRLEMTR
                     YSADIALRAVATMGAGIVAILAGRHARVIVLAEAVVTLILFLWVGAGILARIEIAAAA
                     LLAKAARRLKLAAWRAAVVLMLGSLLSFLTGAVDRWLAAAFLDLQQFGYYAFAWTSIA
                     AAQSFQFLINASLFPLIGRRYWAKSAQDAFRLTAALSLGLAGCGAVAAAMVAPFAGLA
                     VARWFPHQAGAVALIAPLLIAAVLRVADFWSSFLLVVRREALLLKLQIVTLGLAGLVF
                     AAAILASGRPPTAQSLAWLAVGLAAGNFALCGAAAWAHRGALRP"
     gene            4856..>5105
                     /locus_tag="VKU03_09795"
     CDS             4856..>5105
                     /locus_tag="VKU03_09795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71599.1"
                     /translation="MKPIALWQRTPLAGLVNMLRYAARGVRLHPAARIYGDGRRLAIG
                     RGGKIGPGCVFNLIGKGVIRLGEGVWAYRDVEFHTERRI"
BASE COUNT          737 a         1778 c         1674 g          916 t
ORIGIN      
        1 cgccgacaca attaccatcg acgttcattc cagcgacttt acaattagcg atcctgacag
       61 cctgtgggcg ggccgcaatc tctacgatct ctacaaagag gcccacacgc cctgggaatg
      121 gcatgcgccg atcatggtcc gggccaaggc gcgcggcctc gccgcctttt cgacgccgtt
      181 cgacgagagc gcggtcgatt tcctcgaaag gctcggcgtc cccgcctata aaatcgcttc
      241 gttcgagaac gtccacctgc cgctgatccg caaggtcgcc gcaaccggca agccgatcat
      301 tctttcgacc ggcatggcct cgctcgccga gctcgacgaa gccgtggccg ccgcccgcga
      361 ggccggctgc cgcgacctcg tcctgctcaa atgcaccagc acctatccgg cgacgccgga
      421 gaacaccaat ctcgcgacga tcccgcatct gcgcgaattg ttcggctgcg aaatcggcct
      481 gtccgaccac acgatggggc tcggggtcgc ggtcgcggcg acggcgctcg gcgccagcgt
      541 gatcgaaaag cacttcacgc ttcgccgcgc cgatggcggc gtcgacagcg ccttctccct
      601 ggagccggac gaattgcgcg ccctcgtcgt cgagaccggc cgcgctgccg cggcgctggg
      661 caaggtcgcc tacggcccga ccgaagccga gaagaaatcg ctgatcttcc gccgctcgct
      721 ctatctcgtg cgcgatgtgg aggagggcga ggtcctgacc cgcgacaatg tgcggatcat
      781 ccgcccggcc ggcggcctgc cgccgaaagc gatcgacatc gtgctcggca tgaaagcgcg
      841 gcgcaaggcg ccgcgcggcg cgccggtgac ctgggacttg gtgcgatgag ccgccggcgc
      901 ggcgcgtccc gccgggagcg gcgatgatgc gggtcggctt cgccagcatc tattcctggc
      961 gcccgcatgt ctcgatgctg cactacctcg ccggcctcgc ccgccaggcg gggcatgaga
     1021 cctatttcct gacctgcgac ggcgacttgc cgaactgcta cacgcgcgag cagaagccga
     1081 accgcccggc ctggctgaat tgcgctctgt gccgggcggg cggcgtgcgc tcgtatgaaa
     1141 gccgcaatgt cgcttcgatc ggcgcgctcg acggcggcga cgccgcgccg gcgccgcgcg
     1201 cgcgcgaatg ggtcctttcc agcgccagca cgctcggccg gttcgaatcc gacgccgatt
     1261 tcgccggcgc cgaattcgcc ggcttcgccg accgcctcga acccgccgcg cgccgcgcct
     1321 attcggccgc ccgccgctgg atcgacgcca atcgcctcga cgcgatcgtc gccttcaacg
     1381 gccgcatgga cgccacccgc gccattttcg aggcggcgcg cgacgcgcag atacgcttcg
     1441 tcagccagga gcggagctgg ttcggcgacg gcgtccagct cttgcccgac gagaattgct
     1501 tggggctgcg cgccgtcgac aagatgatcg cggcgtggcg ggatcgcccg ttgacgcgcg
     1561 gccaggcttt gcgcgcggcg cgccacgccg ccgcgcgctt cctgcggaag aacgataagg
     1621 agtggcgcgc cttcaatctc gaggcgacca aggcctcctg gccgagcgcc ggcgccgagc
     1681 gccgcattct gcttctgccg gggagccgca acgagacttg gggccacccc gattggcttg
     1741 accaatggcc ggagcggacc gcggccttcg acgcgatcat cgcccgcctt aagctgcggc
     1801 cggaggaggt ccttttgcgc tgccatccga tctggacgga ggcggtgagc gggcgaagcg
     1861 gcgcattacc cgagcgctac tatcgagact gggcggcaaa agcgggcgtg cgcgccattc
     1921 cgagcgctgc gcgcgacagc acgatggact tgatccgcgc ggccgacgcg atcgtcgtcg
     1981 ccggcggcag cgccgccctg gaggccggct tgctcggcaa gcaggtcatc gccgcttcgc
     2041 cgtcgatcta ccagcgagcg ggttttcaga ccacggccta tggtccggcc gatcttgccg
     2101 aactcgcgct gatcacgacc ctgccgccgc ccgcgctacg ggcggagcgc cggcgcatcc
     2161 ggcgattgac tttgcgcttc tgcttcgcag ccgcctatcg cctgccgcaa ttcgtcccgt
     2221 tcgtcgaagc ggcgacgacg acgcgctaca attactattt cggcgccgat ccttggcggc
     2281 tgacgcggtt gctggagagc ggcgaagtgg aggccgacga tcctgcttgc gccgacgacg
     2341 aggcggcgga ggacgccgtc cttgacatga tcgagcgcga agaatgggcg gatatcgacg
     2401 ccgcggcgca aaacgcgccg cggggccgga cgctgaaaaa cgaccgccgc ggtctctatc
     2461 gcgccgtgga ccgggcgagg gagctcatgc cgcggggcga tttatgagcg gcgcttgccc
     2521 aggctccggc gtcggagtag tcgcatgcgc cgttccttgc ggccgcgatg cggctcgccg
     2581 cggcgcgact cttcgtcaga ggagggggat gtgacgaggc tggcgtccat ggtcgccgcg
     2641 ttcagggcca atcccaacta cgcgatgatg cgcggggcgg cgcgattccg cagcatcaac
     2701 ggcgtggtca agggcgccaa aggcctgatc gagggcaggg cctatcgcgc cctgctcgac
     2761 gatgtgaagg cgaggctcgg ccagagccgc ttctcggcgg tcgatcccga cgcgttcgtc
     2821 gccgatttgc ggcgggacgg ctgcgccttc ggcctgaccc ttcccgccgc cgatgtcgag
     2881 gcgatccgcg cctatgcggc gcgcgaaacg gtctatgccg atcgcaattc cagtctgggc
     2941 tttccgctgg cgcggcgact ggaggcggag aaggcgctcg gcaagcccat tcttgtcgcg
     3001 caatatttca acacggaagc gcaatgcccg acgatcgcca agctgcgcgc cgatccgttc
     3061 ctgcactacg cggcggcgcg ctatttcggc agcgcgccct tctctcatga cccgaatctc
     3121 tggtggactt tcgcgaccaa cgcgtccgaa gaggacaaga tcaagcacgc gcatcgcttt
     3181 cattccgatg tcgacgattt cgccatcctg aagtttttct tctacatcac cgatgtcgcg
     3241 gtcggcgacg gctcgcatgt cctggtccgg ggctcgaacc gcgagcggcc ttatcgcaaa
     3301 ttctccgacc gcctcgtcct gcggcgctat atggacgacg agatcacaag cttttatccg
     3361 gcggatcgaa tcgtcgacat tggcgggccg gccgggaccg gcttcgccga ggatacgttc
     3421 tgcatccaca agggcaactc gcctttcgac aagccgcgcc tgctgctgca attccaattc
     3481 acgcttttcg actacgacgc gcagcgcgac agaattccgg ccgagcaact caagcgctta
     3541 ccgctcggat gagcgcggcg ccgctgcgct ttcgaccgat cgcgcctttc atcgagcggg
     3601 cgctgcgctt tttttcctat acgccgctgc tgggcttgtc ggcggtcgtc tcgctcgcca
     3661 agttgatcgt ctatgcgcgc ctggtttcgg ccgacgaata cggcgcgatt gcgcagatgt
     3721 tcctcatcgg cagtactttc ggcgttgtcg gcagcttcgg cttctacctt ttggcgttac
     3781 gcgaattgcc cgggcttcat gtccggcgcc gcgaatttgc gatggcgatc ctgctcggcc
     3841 aggcggctat catctcgacc gcgagcgcgg cggccgcttg cgtccttctg atcccgttcg
     3901 gcgcggccgg cgttttcgcg acgcgaatcg gcggcctcgg aatcgccgtc ggcctctttt
     3961 acgcctggac gcagctctgc tttcagatcg ccacgaccga agcgcgcagc cgtctcgaaa
     4021 tgacgcgcta ctcggcggac atcgccttgc gcgccgtcgc gacgatgggc gccggaatcg
     4081 tcgcgattct cgccggccgc catgctcgcg tgatcgtcct cgccgaggcc gtcgtgacgt
     4141 tgatcctgtt cctctgggtc ggcgccggaa tcctcgcgcg catcgagatc gccgcggccg
     4201 cgcttctcgc aaaggcggcg cgccgcctga aacttgcggc ctggcgcgcg gccgtcgtgc
     4261 tgatgctcgg ctcgctgctt tcgttcctga ccggcgcggt cgaccgctgg ctcgccgccg
     4321 cattcctcga tttgcagcaa ttcggctatt acgcgttcgc gtggacgtcg atcgcggccg
     4381 cgcaaagctt tcaattcctg atcaacgcca gcctgtttcc gctgatcggg cggcgctatt
     4441 gggcgaagtc cgcgcaggac gcgtttcgcc tcaccgccgc cctgtcgctc ggccttgccg
     4501 gttgcggcgc cgtcgccgcc gccatggtcg cgcccttcgc cggcctcgcc gtcgctcggt
     4561 ggtttccgca tcaggccgga gcggtcgcgc tgatcgcgcc gctcctgatc gccgccgtgc
     4621 tgcgcgtcgc cgatttctgg tcgagctttc tgctcgtcgt gcggcgcgag gccttgcttc
     4681 tcaaattgca gatcgtcact ctcggcttgg ccgggctcgt ctttgcggcc gcgatattgg
     4741 cgtccggccg gccgcccacg gcgcaaagcc tggcctggct cgccgtcggc ctcgccgccg
     4801 ggaatttcgc gctctgcggc gcggcggctt gggcccatcg gggagcgctg cgtccatgaa
     4861 gccgatcgcg ctgtggcaaa ggacgccgct ggccggcttg gtcaatatgc tgcgatacgc
     4921 cgcgcgcggc gtccgccttc atcccgcggc gcggatctac ggcgacgggc gccgtctcgc
     4981 gatcggacgc ggcggcaaaa tcggccccgg ctgcgtgttc aacctcatcg gcaagggcgt
     5041 cattcggctc ggcgaaggag tctgggctta ccgcgatgtc gaatttcaca ccgagcgccg
     5101 gatcg
//
LOCUS       DATGJW010000320         3177 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_165072, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000320 DATGJW010000000
VERSION     DATGJW010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3177)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3177)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3177
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165072"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..569
                     /locus_tag="VKU03_09800"
     CDS             <1..569
                     /locus_tag="VKU03_09800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0010945 - coenzyme A diphosphatase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA pyrophosphatase"
                     /protein_id="HLJ71600.1"
                     /translation="LDPAHAPKRGDFSLNGPGPDAALLSTARPAAVLAPIVARPGGAT
                     VLLTQRATALRQHSGQIAFPGGKIDPDEAPLAAALREAQEEIGLDARHIEPTGWLDPY
                     LTGTGFLILTMTAIVTPDFTLSINPYEVEDAFETPLSFLMDAANHQRQSREWQGRLRH
                     FYVMPYENRNIWGATAGILRNLYERLYS"
     gene            569..832
                     /locus_tag="VKU03_09805"
     CDS             569..832
                     /locus_tag="VKU03_09805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6111 family protein"
                     /protein_id="HLJ71601.1"
                     /translation="MLRAVLEPVALFLSPFILFAIYLALQARYPLAMEHWTRSRVSTL
                     TLVGLFVAILGMVILALYAPRGQGTYIPAHLNKSGVFVPGHIE"
     gene            829..2109
                     /locus_tag="VKU03_09810"
     CDS             829..2109
                     /locus_tag="VKU03_09810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CCA tRNA nucleotidyltransferase"
                     /protein_id="HLJ71602.1"
                     /translation="MSALTTLVEGRLADDKLLRKGGLASILDALDGRGEETRVVGGAV
                     RDLVLGLKVSDFDLATTATPDETMRRARAAGLKAVPTGLLHGTVTIVVDGQGYETTTL
                     REDVETDGRHAKVRFGRDFKQDALRRDFTINALSLSRDGRVHDYAGGLADLAARRVRF
                     IGEARQRIREDYLRALRFFRFSAAYGDSPIDEEGFLAAVQERDGLQRLSRERIAAELF
                     KLLKAPRAAEVMTTLCNCGLLIPLLAAAPDPARHARLVAIERARGAPPDPLLRLAGLM
                     VRIVEDADRLRERLRLSNADLDRLSEAAHVLETLHGLSEPPPPGDLRALLYAHGRTAA
                     LDALTLAHADSGAAPDDKRFLSTHYFLLDTPMPALPFSGAEILARGVATGHQVGATLK
                     ALQALWIRAGFPQEPEKLAALLDEALARARGERR"
     gene            2383..>3177
                     /locus_tag="VKU03_09815"
     CDS             2383..>3177
                     /locus_tag="VKU03_09815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71603.1"
                     /translation="MALRIMLTSRQPIWVGWGQDLIYFYNDPYKSIIGGKHPWALGRP
                     TKEVWREIWPEIGPMLATAMSGDAGTYVEAQLLIMERNGYPEETYYTFSYSPIPNDDG
                     TVGGIICANSDDTQRVIGERQLALLRDLAAGATHTRTWQEACERSAEALSTNARDIPF
                     SMIYLEEPDSAVMKLAGVSGIERGAAIAAEEMSPEASAPWPFMEVLRTHQLAIVADLQ
                     NTVGAELPSGPWSAAPTHAAVIPLSPPGETGRAGVLIVGLNPFRLFD"
BASE COUNT          542 a         1047 c         1016 g          572 t
ORIGIN      
        1 cgctcgatcc cgcccatgcg ccgaagcgcg gcgatttctc tttgaatggg ccgggtcctg
       61 acgcggcgct tttgagcacg gcgcggccgg cggcggttct cgcgccgatc gtcgcgcgac
      121 cgggcggcgc gacggtgctg ttgactcagc gcgcgacggc gctccgccag cattccggcc
      181 agatcgcctt tcccggcggc aagatcgatc ccgacgaggc gccgctggcc gcggccctgc
      241 gcgaagcgca agaagaaatc gggctcgatg cgcgccatat cgagccgacc ggctggctcg
      301 atccgtacct caccggcacc ggcttcctca ttctgacgat gacggccatc gtcacgcctg
      361 atttcacgct ttcgatcaat ccttatgaag tcgaggacgc tttcgaaaca ccgctcagct
      421 ttttgatgga cgccgccaat catcagcgcc aatcgcgcga gtggcagggg cggctgcggc
      481 atttctacgt catgccttat gagaaccgaa acatttgggg cgcgacggcg ggcatcctgc
      541 gcaatcttta cgagaggctg tactcctgat gctgcgcgca gtgctcgagc cggtcgcgct
      601 gttcttgagt cccttcatct tgttcgcgat ctatctcgcg ctgcaggcgc gctacccgct
      661 cgccatggag cattggacgc gcagccgcgt ctccaccctg acgttggtcg gcctcttcgt
      721 cgccatcctc ggcatggtga ttctcgcgct ttatgcgccg cgcggccaag gcacctatat
      781 tcccgctcat ctcaacaaga gcggcgtctt cgtgcccggc catatcgaat gagcgcgctg
      841 acgactcttg tcgagggacg actcgcagac gacaagctgc tgcgcaaggg cggcctcgcc
      901 agcatattgg acgcgctcga tggacgcggc gaagaaaccc gcgtcgtcgg cggcgccgtg
      961 cgcgacctcg ttctcggcct gaaagtgagc gatttcgatc tcgccaccac cgcgacgcct
     1021 gacgaaacga tgcgccgcgc ccgcgccgcc ggattgaaag ccgtaccgac gggcctgttg
     1081 catggtacgg tgaccatcgt cgtcgacggc caaggctacg agacgacaac cttgcgcgaa
     1141 gacgtcgaaa cggacggacg ccacgccaaa gtacgattcg gccgcgactt caagcaagac
     1201 gcgctacgcc gcgatttcac catcaacgcc ttatcgctgt cgcgcgacgg ccgcgtccat
     1261 gactatgccg gcggcctcgc cgatcttgcc gcccggcgcg tgcgcttcat cggcgaggcg
     1321 cgccagcgca tccgagagga ctatctgcgc gccttgcggt tctttcgctt ttccgccgct
     1381 tacggcgaca gcccaatcga tgaggaaggg tttctcgcgg cggttcaaga acgcgacggg
     1441 ttgcaacgcc tgtcgcgcga gcgcatcgcg gcggaattgt tcaaactctt gaaggcgccg
     1501 cgggcggccg aggtcatgac gacgctctgc aattgcggcc tgctgatccc gcttctcgcc
     1561 gcggcgcccg acccggcgcg tcacgcgcgg ctcgtcgcta tcgaaagggc gcgcggcgcg
     1621 ccgcccgatc cgctgctgcg cctcgccggg ctgatggtac ggatcgtcga ggacgcggac
     1681 cgtttgcgcg aacggctgcg gctgtccaac gccgacctcg atcggctgag cgaagcggcg
     1741 catgtgctcg agactttgca tggtttgagc gaaccgccgc cgccgggcga tctgcgcgct
     1801 ttgctctacg cgcatggacg cacggccgcc ctcgacgcgc tgacgctcgc ccatgccgat
     1861 tcgggcgcgg cgccggacga taagcgcttt ttgagcacac actatttcct cctcgacacg
     1921 ccgatgcccg ccctgccctt ctccggcgcg gaaatcctcg cccgcggcgt cgcgaccggc
     1981 catcaggtcg gcgcgacgct gaaagcattg caggcgctgt ggatccgggc gggatttccg
     2041 caggagccgg agaaactggc ggcgctcctg gacgaggcgc tggcgcgagc gcgcggggag
     2101 aggcggtgac gatagtgtcg tcaattcagc gtcataagct ccctcaaagg atcaaaatgg
     2161 ctcgttgagc gtttgagtcg ccaacgtcaa accgctaggt ttgaatgccg ccgggggagc
     2221 gtgttggtga ataaggaatt gcgcgggggc gcggatgcgc gctcgaatga caacgcgcgg
     2281 agttttctcg ccggaggcgg cgagctgggc caactcacgc gcgattttga ctgggtcgcc
     2341 acgcctttgg gcgcgccgga aaattggccg cagaacctca gaatggcgct tcgcatcatg
     2401 ctgacctccc gtcagccgat ctgggtcggc tgggggcagg acctgattta tttctataac
     2461 gacccttaca aatcgatcat cggcggcaag catccctggg cgctcggcag gccgacgaag
     2521 gaagtctggc gcgaaatctg gccggagatc gggcccatgc tcgccaccgc gatgagcggc
     2581 gatgcaggaa cctatgtcga agcccagctc ctcatcatgg agcggaacgg ctatcccgaa
     2641 gaaacctatt atacgttctc gtatagtcca attcccaatg acgacgggac ggtcggcggc
     2701 attatctgcg ccaatagcga cgatacccaa cgcgtcatcg gcgaacgcca gctcgctctc
     2761 ttgcgcgatc tcgcggccgg agcgacccat acgcgcactt ggcaggaggc gtgcgagcga
     2821 agcgcggaag ccttgtcaac gaacgcccgc gatatcccgt tttcgatgat ctacctcgag
     2881 gagccggaca gcgccgtcat gaagttggcg ggcgtttcgg gcattgaacg cggcgccgcg
     2941 atcgccgcag aagagatgtc gccggaggcg tccgcgccat ggcccttcat ggaagtgttg
     3001 cggacgcatc aactggcgat cgtggcggat ttgcagaaca ccgtcggcgc ggagctgcca
     3061 agcggccctt ggagcgccgc gccgacccac gccgcggtga tcccgctctc gccgcccggc
     3121 gagaccggcc gcgccggcgt tctgatcgtg ggactcaatc cctttcgttt gttcgac
//
LOCUS       DATGJW010000321         4305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1410272, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000321 DATGJW010000000
VERSION     DATGJW010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4305
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1410272"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            212..1708
                     /gene="hisS"
                     /locus_tag="VKU03_09820"
     CDS             212..1708
                     /gene="hisS"
                     /locus_tag="VKU03_09820"
                     /EC_number="6.1.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004821 - histidine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006427 - histidyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine--tRNA ligase"
                     /protein_id="HLJ71604.1"
                     /translation="MTQEKKTSLVTPELPRGLIDRPASDIAATEAMMAKIRAVYELYG
                     FEAVETPFIEYTEALGKFLPDLDRPNEGVFSFKDDDERWLSLRYDLTAPLARYVAENY
                     ERLPKPYRSYRAGWVFRNEKPGPGRYRQFMQFDADTVGAASPAADAEICMMAADCLEA
                     LGIKRGDYVIKVNNRKVLDGVMEAIGLGGEENAGRRLTVLRAIDKYDRLGLAGVRQLL
                     GAGRKDESGDFTKGAGLAGDQAELVLGALNIGDRKDEARAREHLNLGGVGREGAGELA
                     EIGRLVAAGGYSDRVRIDPSVVRGLEYYTGPVFEAELTFETPGEDGKPIRFGSVGGGG
                     RYDGLVARFRGENVPATGFSIGVSRLLAALRAIRSPIVGAARKTGPVVVVALDRDHMA
                     DYQRLVAELRRADIAAELYLGQGGMNAQLKYADRRGSLCAVIQGSNERDAPGGRQVVI
                     RDLVLGAELAKATKDRADYLELRQRAQQAVPESDLVARVQDVLNRHRR"
     gene            complement(2227..3084)
                     /locus_tag="VKU03_09825"
     CDS             complement(2227..3084)
                     /locus_tag="VKU03_09825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018391439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HLJ71605.1"
                     /translation="MTGAGTDRGKQALAGGLAVVLVKPQLAVNIGMCARAMANFGLDD
                     LRLVDPRGGWPREATWAKAAYAAAASAGALLDRAKVFATVKEAVADLNYVYATTARER
                     GQLKRVFTPAEAMPEAASAVAGGEKRGVLFGPERTGLDNDDVALADSILTFPVSPGYA
                     SLNLAQAVLLCGYEWYKAAHGGAAPIAAPERSPPATREMVLAFFEMLEGHLEARGFFK
                     PAPKKPVMQRNLRNIFHRIGMTQQDVRTLWGAIVRLVEGPRVTVQTRKRRRSPRLAQR
                     GENGGEAAG"
     gene            complement(3224..4210)
                     /locus_tag="VKU03_09830"
     CDS             complement(3224..4210)
                     /locus_tag="VKU03_09830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mitochondrial fission ELM1 family protein"
                     /protein_id="HLJ71606.1"
                     /translation="MTRALVKDGTRVLILSTGMAGHDVNCLGVAAALGVAYRKIVVAP
                     RRLFRSLAPYGPVDPKDRSGRPGSILAPPFPDIVIASGRSTVPYIRALKREGGRDVFA
                     VFLQDPRYGRREFDLIWTPLHDRLAGANVIATLTSPHPFSAARLEALRAAPDPRLAAL
                     PAPRAAVLLGGPSSAHDFSASDLDRLGDSIAAILRQGYSVMATPSRRTPPPLLETARR
                     ALQAADAKRAFVWDGTGDNPYGQMLAQADAILVTGDSVNMMGEAVSTGAPVYIFAPSG
                     GGRRIDAFVAALEEKGAARRFEGRIEPFAYEPIDSSLEVANAIAERFAAGRH"
BASE COUNT          728 a         1467 c         1458 g          652 t
ORIGIN      
        1 ggtttgagat cggaagattc gtgccgcggc ttaccctcac cccaaccctc tcccgcaagc
       61 gggagagggg gcgccgagcg cttaaagcgg cctcgatgtt catcgctcgc cgccggagaa
      121 ccccttctcc cgcttgcggg agaaggtggc gcgaagcgcc ggatgagggt aagcctccgc
      181 gccaatcgtt ctagaaatct ttccggccca gatgactcaa gaaaaaaaga cttccctcgt
      241 cacccccgaa ttgccgcgcg gcctcatcga ccgcccggcg tcggacatcg ccgcgaccga
      301 ggcgatgatg gcgaaaatac gcgccgtcta cgagctctac ggcttcgagg cggtggagac
      361 gccgttcatc gaatataccg aggcgctcgg caagttcctg cccgatctcg accggccgaa
      421 cgagggcgtc ttctcgttca aggacgacga cgagcgctgg ctatcgctgc gctacgactt
      481 gaccgcgccg ctcgcgcgct acgtggcgga gaattacgaa aggctgccca agccctaccg
      541 ctcgtaccgc gccggctggg tgttccgcaa cgagaagccg gggccgggac ggtaccggca
      601 gttcatgcag ttcgacgcgg acacagtcgg cgcggcgtcg ccggcggcgg acgccgagat
      661 ctgcatgatg gcggcggatt gcctggaggc gctggggatc aagcgagggg attacgtcat
      721 caaggtgaac aaccggaagg tgcttgacgg cgtgatggag gcgatcgggc tgggcgggga
      781 ggaaaatgct gggcggcggc tcacggtgct aagggccatc gacaaatacg atcggctcgg
      841 ccttgccggc gttcgccaac tcctcggcgc cggccgaaag gacgaaagcg gcgacttcac
      901 gaaaggcgcc gggctcgccg gcgatcaggc cgagctcgtc cttggcgctt tgaacatcgg
      961 cgatcgaaaa gacgaggccc gggcgcgcga gcatttgaac ctcggcggcg ttggccgcga
     1021 gggagccggc gagctcgccg agatcggaag gctggtcgcc gccggaggtt attccgatcg
     1081 cgtccgcatc gacccctccg tcgtccgcgg cctcgaatat tacaccggcc cggtcttcga
     1141 ggccgagctc accttcgaga cgcccggcga ggacggcaag ccgatccgct tcggctcggt
     1201 cggcggcggc ggccggtacg acgggctcgt cgcgcgcttc cgcggcgaga atgtcccggc
     1261 aaccggcttc tcgatcggcg tctcgcgcct gctcgcggcg ctgcgcgcga tcaggagccc
     1321 gatcgtcggc gcggcgcgta agaccggccc ggtcgtcgtc gtcgcgctcg accgcgacca
     1381 tatggccgac taccagcgtc tcgtcgccga attgcgccgc gccgacatcg ccgctgagct
     1441 ctatcttggg cagggcggca tgaacgccca gctcaaatac gccgaccgcc gcggcagcct
     1501 ctgcgccgtc atccaaggct cgaacgagcg cgacgcgccg ggcggacggc aagtggtcat
     1561 ccgcgacctt gtgctcggcg cggagctggc gaaagcgacc aaggaccgcg ccgattatct
     1621 ggagctgcgc cagcgcgcgc aacaagccgt acccgaaagc gacctcgtcg cccgtgtaca
     1681 ggacgtgctg aaccggcaca ggcgttgaga atcccttgtc cccgccttgc gctacggcac
     1741 gcaggcatct cggcgcgggg ccacatcggc ccggcatcgt caagcggcca tagcccgacc
     1801 gaccgttgga cgaaccgacc aagctctgcg ccgcgggcta tggccgctgc gccgcttcgc
     1861 ttcgcccctt cgggtgacga cgccgggccg atgcggcttt ttgcatccaa gagctttgct
     1921 gacgaaaccg tcggaagccc gacggttcgt cagcaaagct cttggaagcc aaaccgcctg
     1981 agccgcgcgg agccgtcaag gctcgcgagc catcgcgccg cgccactgat tttctcctga
     2041 gcctgctttg gcgagcgacg cgcgcagcgc gcttggcctt gacggcgagc acggctcagg
     2101 cacaccgcgc ttcaatgcgt gcatgccgta tcgccttgca cagagaaggt ggcgcgaagg
     2161 ggcggcgcgg gactcgaagg gtacgcgccg ttcgaaacgc caaccggcaa gctcggcgca
     2221 gccccctcac ccggcggctt cgccgccatt ctctccacgc tgcgcgaggc gaggggatcg
     2281 gcgccgtttc cgcgtctgca cggttacgcg cggcccttcg accaggcgca cgatcgcgcc
     2341 ccaaagcgtg cgcacgtcct gctgggtcat gccgatgcgg tggaagatgt tgcgcaaatt
     2401 gcgctgcatc accggctttt tcggcgcggg cttgaagaag ccgcgcgctt cgagatggcc
     2461 ttcgagcatt tcgaaaaagg cgaggaccat ttcgcgcgtc gccggcggcg agcgctcggg
     2521 cgcggcgatc ggcgccgcgc cgccatgcgc ggccttgtac cattcatagc cgcagagcag
     2581 aaccgcctgg gcgaggttga gcgaggcgta gccggggctg accggaaagg tgaggatcga
     2641 atcggcgagc gccacgtcgt cattgtcgag gccggtgcgc tcagggccga acaggacgcc
     2701 gcgcttctcg ccgcccgcca cggcgctcgc cgcctccggc atcgcctcgg ccggcgtgaa
     2761 gacgcgcttc aactggccgc gctcgcgcgc cgtcgtcgcg tagacatagt tgagatcggc
     2821 gaccgcctcc ttgacggtgg cgaagacctt ggcgcggtcg agcagcgcgc cggcgctcgc
     2881 cgccgccgca taggcggctt tcgcccaggt cgcctcgcgc ggccaacccc cgcgcggatc
     2941 gaccaggcgc aagtcgtcga gaccgaaatt ggccatcgcc ctggcgcaca tgccgatatt
     3001 gaccgcgagc tgcggcttga ccagcaccac ggcgagaccg ccggcgagcg cctgcttgcc
     3061 gcgatcggtt ccggcgccgg tcattcgtgg ttctccggcg cgaggcgacg cagccctatc
     3121 ggcccggcgt cattgcgagc gcagcgaagc aatccgttcg gagcgctgaa ctcgaagaac
     3181 ggtcgtaatg agggaatcag cttcgcttct cgcaatgacg gaatcaatgg cgccccgccg
     3241 caaaccgctc cgcaatcgcg ttggcgacct ccaggctcga atcgatcggt tcgtacgcga
     3301 agggctcgat ccttccctcg aaccgccgcg ccgcgccttt ctcctccagc gccgcgacga
     3361 aagcgtcgat gcgccgcccg ccgccgctcg gcgcgaaaat atagaccggc gcgccggtcg
     3421 agacggcttc gcccatcata ttgacgctgt cgccggtgac gaggatggcg tcggcctgcg
     3481 ccagcatctg cccgtaagga ttgtcgccag tcccgtccca gacgaacgcc cgcttcgcgt
     3541 cggccgcctg cagcgcccgc cgcgccgttt ccagcagcgg cggcggcgtg cggcgcgacg
     3601 gcgtcgccat cacgctatag ccttggcgca gaatcgccgc gatcgagtcg cccaggcgat
     3661 cgagatcgct cgccgagaaa tcatgcgcgc tgctgggccc gccgagcagg acggccgcgc
     3721 gcggcgccgg taaggcggcg agacgcggat cgggcgcggc gcgaagcgct tcgagccgcg
     3781 ccgccgagaa aggatgcggc gaggtcaagg tcgcgatcac attcgcgccg gcaaggcggt
     3841 cgtgcagcgg cgtccagatc aggtcgaatt cgcgccgccc atagcgcggg tcttgcagga
     3901 agacggcgaa aacgtcgcgc ccgccttcgc gcttgagcgc gcggatatag ggcacggtcg
     3961 accgtccgct ggcgatgacg atgtcgggaa agggcggcgc gagaatcgag ccgggccggc
     4021 cagaccggtc tttcggatcg accgggccgt aaggcgccag gcttctaaac agcctgcgcg
     4081 gcgcgacgac gattttccga taggcgacgc caagcgccgc cgcaacgccc aggcaattga
     4141 cgtcgtgtcc cgccatgccc gtggaaagaa taaggacgcg cgtaccgtcc ttaaccagcg
     4201 cgcgcgtcat tcagcttctc ggtcattgcg aggagcgaag cgacgaagcg acccgttcat
     4261 gaagctgcgc ctgataagtg gattgcttcg ctcgcaatga cgggc
//
LOCUS       DATGJW010000322         6883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_891404, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000322 DATGJW010000000
VERSION     DATGJW010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6883
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_891404"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            326..796
                     /gene="purE"
                     /locus_tag="VKU03_09835"
     CDS             326..796
                     /gene="purE"
                     /locus_tag="VKU03_09835"
                     /EC_number="4.1.1.21"
                     /EC_number="5.4.99.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009320 - phosphoribosylaminoimidazole
                     carboxylase complex [Evidence IEA];
                     GO_function: GO:0004638 - phosphoribosylaminoimidazole
                     carboxylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide mutase"
                     /protein_id="HLJ71607.1"
                     /translation="MGSQSDWTTMRRAASTLDDFGVAYDARIVSAHRTPDRLAVFAKS
                     ARDAGFKVIIAGAGGAAHLPGMVAAFTPLPVLGVPIQSKALSGRDSLLSIVQMPAGAP
                     VGTLAIGEAGAVNAALLAVAILALENPALAARLDAWRRAQSEAVAERPTESDAR"
     gene            793..1878
                     /locus_tag="VKU03_09840"
     CDS             793..1878
                     /locus_tag="VKU03_09840"
                     /EC_number="6.3.4.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="5-(carboxyamino)imidazole ribonucleotide
                     synthase"
                     /protein_id="HLJ71608.1"
                     /translation="MRLSPGATIGILGGGQLGRMLAMAAARLGLKSHIFAPEADAPAF
                     DVAAAQTVAAYDDEKALGEFAEAVDVVTYEFENVPIACAEYLSERRPLRPNARALALT
                     QDRLPEKTFLRDIGLKTAPFLPVEDAGALVRAVGSLGRPSILKTRRFGYDGKGQALVR
                     EGSDLAALFRGLGGEPAILEGFVRFEREVSLIATRGLDGDFAAFDLCENEHERHILAR
                     THLPASVSGATEAAAREIARQIVEALDYVGVLAVEMFVVRDHDGAERLIVNELAPRVH
                     NSGHWTIDGALTSQFEQHVRAIAGWPLGATARLGAIEMRNLIGEEAAAWLDILAEPGL
                     RLHLYGKLEARPGRKMGHVTRIAPRQG"
     gene            2063..2305
                     /gene="rpsU"
                     /locus_tag="VKU03_09845"
     CDS             2063..2305
                     /gene="rpsU"
                     /locus_tag="VKU03_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011159711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S21"
                     /protein_id="HLJ71609.1"
                     /translation="MQVLVRDNNVDQALKALKKKMQREGIFREMKLRGHYEKPSEKRA
                     REKAEAVRRARKLARKKLQREGLLPVKAKPSPGAPR"
     gene            2496..3341
                     /locus_tag="VKU03_09850"
     CDS             2496..3341
                     /locus_tag="VKU03_09850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ71610.1"
                     /translation="MASGSGNSARGALALLAAFALAGCNSLGMASAPPDAHVAEVEPD
                     QTGASAVNIASLSDVIQRNPNDSAAYNTRGAAYARIGRYQNAIDDFTHAVQIDPNFVA
                     AYVNRALAYRQIRKDNLALADFNQALAVNPNHAPAYLGRANLLRAQGHLPEALADLNQ
                     AIRLNPEGAQAFHARGLIYQRQGDNARAITDFNNAIDRDPFAGAPYQARGESYLALGK
                     YDAAIEDFNAALNVDAHNADAWAGLGVAYEKQNNRSKAVESYGRAIAVDKNNQVAREG
                     LDRLR"
     gene            3646..4863
                     /locus_tag="VKU03_09855"
     CDS             3646..4863
                     /locus_tag="VKU03_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="divergent polysaccharide deacetylase family
                     protein"
                     /protein_id="HLJ71611.1"
                     /translation="MTGFDQHDDLNKPLGLGAAKAKSRPPLSALLATAGVLAATGLVA
                     FLARTGDRLAGEPYAVATITRPAPASVPASSPRAPPASGGIESLSVSQVEAQSGVKVV
                     RNGGARPPGALIIEVPETIGVHLNPAPDERLVERSRYGLLPRIGADGARPADVYARPS
                     LVPANLKNAPRVALLVGGLGLSEEGTSQAIRQLPGAVTLGFAPYGAAVEAEVAEAREA
                     GHEAILQIPMEPFDYSANNPGPHTLLTSTGERQTLDDLHWLMARFQGYVGIANFLGAK
                     FTADEAALSPVLRDIAGRGLLYVDDGSSPRSLARALAGRLDLPAVTADVVIDAVPTPQ
                     AIDAALTRLEALARANGSALGVASALPTSVEHISRWARALEGRSLALTPVSAIVTRAP
                     GPAAENAAPALPR"
     gene            4868..5383
                     /locus_tag="VKU03_09860"
     CDS             4868..5383
                     /locus_tag="VKU03_09860"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA pyrophosphohydrolase"
                     /protein_id="HLJ71612.1"
                     /translation="MRFEDLPYRPSVGVALFNREGMVFLGRRKKRRVASKDSIGHEWQ
                     MPQGGIDKGETPRAAALRELYEETNVESVSPLAEAPQWLTYDLPPDVAANRWRGRFRG
                     QRQKWFAFRFEGEDSEIDIDRPAKGAHPPEFDAWRWERIEELPALVIPFKRNVYEVVV
                     ATFAPLVKPAG"
     gene            5614..6237
                     /locus_tag="VKU03_09865"
     CDS             5614..6237
                     /locus_tag="VKU03_09865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012318734.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase family protein"
                     /protein_id="HLJ71613.1"
                     /translation="MPAPFNKLLREIRACRVCRDAPRSGRPLPHEPRPIIQASIEARL
                     CIAGQAPGVRVNASGRPYDDPSGVRLRQWLGIDEATFYDPKKIAVVPMGFCFPGLDAK
                     GGDLPPRKECAATWRRRIFDALPRIELILLVGLYAQKWHLGAAATAEGLSGTVGKWRD
                     IYRPKDQRRLLPLPHPSWRNNVWLKRNPWFEAELLPVLRGEVSRLTA"
     gene            complement(6243..6563)
                     /locus_tag="VKU03_09870"
     CDS             complement(6243..6563)
                     /locus_tag="VKU03_09870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116062.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1476 domain-containing protein"
                     /protein_id="HLJ71614.1"
                     /translation="MGAFEEREEGFEKRFAADEALSFKARARRNRLLGLWAAQLTGKQ
                     GAEAESYAKALVAAQVESADDEALFKTLRADLSAAGVDISDHRIRRKMSEALAQARRE
                     IAAG"
     gene            complement(6683..>6883)
                     /locus_tag="VKU03_09875"
     CDS             complement(6683..>6883)
                     /locus_tag="VKU03_09875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HLJ71615.1"
                     /translation="GRLVFPPRGVHGFGYDPIFVPDGHARGFGEMTAEEKHGIPADGS
                     RALSHRARAFQDLARACLDRRR"
BASE COUNT         1122 a         2376 c         2236 g         1149 t
ORIGIN      
        1 cggaaatcgg gccgatttcc cggtcaaatg ttttcagtgg cttagcgtcg attttctttc
       61 cctccgtcga tttgaccaga tttttggcgg ccatcgcggg aagccccgta ttataattga
      121 tttctgaaat ttagaaagag aattcgacgc agcggcggac gattttctcg gcggcggttc
      181 gaaagcgcgg cgccaccgaa aggtacgacc ccatgcggcc cgccagcgcc gccttgcgcc
      241 tcgcctttca ctggtttaag cgcaccgcct tacgccagca tcgagaggct ccgtggcgcc
      301 ttcgcaaaag cccgtcgccg tgctcatggg aagccagtcc gactggacga cgatgcggcg
      361 cgccgcctcg accttggacg atttcggcgt cgcttacgac gcgcgcatcg tctccgccca
      421 ccgcacgccg gaccgcctcg ccgttttcgc caaaagcgcg cgcgacgcag gtttcaaggt
      481 gatcatcgcc ggcgccggcg gcgccgcgca tctgcccggc atggtcgcgg ctttcacgcc
      541 gctgccggtc ctcggggtgc cgatccaatc caaagctttg tccgggcgcg acagcctgct
      601 ctcgatcgtg caaatgccgg ccggggcgcc ggtcgggacc ttggcgatcg gcgaagccgg
      661 cgccgtcaat gcagcgctgc tcgccgtcgc catcctggcg ctcgaaaacc cggcgctggc
      721 ggcgcggctc gacgcctggc ggcgcgccca gagcgaggcc gtcgccgagc ggccgacgga
      781 aagcgacgcc cggtgaggct ttctcccggc gcgacgatcg gcatcctggg cggcggccag
      841 ctcgggcgca tgctcgccat ggcggcggcg cggctcggcc tcaagagcca tattttcgcg
      901 cccgaggccg acgcgccggc tttcgacgtc gcggccgccc agaccgtcgc cgcctatgac
      961 gacgaaaagg cgctcgggga attcgccgaa gcggtcgacg tggtcaccta cgaattcgag
     1021 aacgtgccga tcgcctgcgc cgaatatttg agcgagcgcc gcccgttgcg gcccaacgcc
     1081 cgggcgctgg cgctgaccca ggaccgcctg ccggaaaaga ccttcctgcg ggatatcggc
     1141 ctgaagaccg cgcccttcct gcccgtcgag gacgccggcg ccctggtgcg cgcggtcggc
     1201 tcgctcggcc gtccgtcgat cctgaagacg cggcgtttcg gctatgacgg caagggccag
     1261 gcgctggtgc gcgaaggctc cgacctcgcc gcgctgttcc gcggccttgg cggcgagccg
     1321 gccatcctgg aaggcttcgt gcgcttcgag cgcgaagtct cgctgatcgc cacgcgcggc
     1381 ctcgacggcg atttcgccgc cttcgatctt tgcgagaacg agcatgagcg ccacatcctc
     1441 gcccgcaccc atcttcccgc gtcggtgagc ggcgcgacgg aggcggcggc gcgcgagatc
     1501 gcgcgccaga tcgtcgaggc cttggactat gtcggcgttc tcgcggtcga gatgttcgtc
     1561 gtgcgcgacc atgacggcgc ggagcgcctg atcgtcaacg aactcgcccc gcgcgtccac
     1621 aattccggcc attggacgat cgacggcgcg ctgacctcgc aattcgagca gcatgttcgc
     1681 gccatagccg gctggccgct cggcgcgacg gcgcggctcg gcgcgatcga gatgcgcaac
     1741 ctgatcggcg aagaggcggc ggcctggctc gacattctcg ccgagcctgg gcttcgcctc
     1801 catctttacg gcaagctcga agcgcggccc ggccgcaaga tggggcatgt cacccggatc
     1861 gcgccgcgcc aaggctagag cgcgagacgg attgggcggc aattttcggg ccaaagccgc
     1921 tcgcggctct ggacaatgac ttgcggctct ggtaaacgcg cgctccttcg gccctgcgct
     1981 cgaagcgcat gcgccggcca ttcattttcg tgagtttctc gtccagcaag gctttgggaa
     2041 agcctcgaac acaggatcgc tcgtgcaagt tctcgtccgc gacaacaatg tcgatcaggc
     2101 cctcaaggcg cttaagaaaa agatgcagcg cgagggcatt ttccgcgaga tgaagctcag
     2161 gggccattac gagaagccgt cggaaaagag agcccgggaa aaggccgagg cggtgcgccg
     2221 cgcccgcaag ctggcgcgca agaaattgca gcgcgagggg ctgttgccgg tcaaggccaa
     2281 gccgagcccc ggcgcgccgc gctgagggcg ctcttcgccc agtgtttcgc cgtggccgca
     2341 gccgaagggc tcgcggccat gatcgttttt aggccggttc aggccaaaat ttcgattttg
     2401 ttagccactt tgcagacaag atcggcgcgc tttaaaaggt cgtaacctta atggcgccgc
     2461 gaattgcgcg cctcatcgag cggagcttga aagacatggc gagcggttcc ggcaattcgg
     2521 cccgcggggc gctcgctttg ctcgccgcct tcgctttggc cggctgcaac agcctcggca
     2581 tggcgagcgc gccgcccgac gcgcatgtcg ccgaggtcga gcccgatcag accggcgcca
     2641 gcgccgtcaa catcgcttcg ctcagcgacg tcatccagcg caatcccaac gattctgcgg
     2701 cgtacaatac gcgcggcgcg gcttacgcgc gcatcggccg ctaccaaaac gcgatcgacg
     2761 atttcaccca tgcggtgcag attgatccca atttcgtcgc cgcctatgtc aaccgcgcgc
     2821 tcgcctatcg ccagatccgc aaggacaatc tcgcgctggc cgatttcaat caggcgctgg
     2881 cggtcaatcc caatcacgcg cccgcttatc tcggccgcgc caatctcttg cgcgcgcaag
     2941 gccatttgcc ggaggcgctc gccgatctca atcaggcgat tcggctcaat ccggaaggcg
     3001 cgcaggcttt ccatgcgcgc ggcctgatct atcagcgcca gggcgacaac gcccgcgcga
     3061 tcaccgattt caacaacgcc atcgaccgcg atcctttcgc cggcgcgccc taccaagcgc
     3121 gcggcgagag ctatctggcg ctcggcaaat acgatgcggc gatcgaggat ttcaacgccg
     3181 cgctgaatgt cgacgcgcat aacgccgacg cctgggccgg actcggcgtc gcctacgaaa
     3241 agcagaacaa ccgctccaag gcggtcgagt cctatggccg cgcaatcgcc gtcgacaaga
     3301 acaaccaggt cgcgcgcgag gggctcgacc ggctgcgcta acggagccgt ttgccgtcat
     3361 tgcgagcgaa gcgaggcaat ccaatcttcg cgcgaatgga ttgcttcgtc gctccgctcc
     3421 tcgcaagtga cggaaatctg ctccgcgcaa atagcgaagg tacgccggga aagcgccggg
     3481 ttggtccccc gctccaagcg ctccgaagtc ccacacggcg taaggcggct tgccgccgct
     3541 cggccaattg gcgaaagttt ccctcgaccg attcgtcggc cgcgccgaca ataggcgcgg
     3601 gtgattcgct ttagacgcct tgcggccggg ccgcggaacg gggcgatgac gggctttgat
     3661 cagcacgacg atttgaacaa gccgctcggc ctcggcgcgg cgaaggcgaa gagccgccct
     3721 cctctttcgg cgcttctggc gaccgctggc gtcctcgccg cgacgggatt ggtcgccttt
     3781 ctggccagaa ccggggatcg gctggccggc gagccctatg cagtggcgac gatcacccgg
     3841 ccagcgccgg ccagcgtccc cgcttcttcc ccacgtgcgc cgccggcctc cggcggcatc
     3901 gagtcgcttt cggtcagcca ggtcgaggcg caaagcggcg tcaaagtggt gcgcaatggc
     3961 ggcgcgcggc cgcccggcgc gctgatcatc gaagttcccg agacgatcgg ggtccatctc
     4021 aatcccgccc cggacgagcg gctggtcgaa cgatcgcgtt atggcctgct gccgcggatc
     4081 ggcgccgacg gggcgcgacc ggccgacgtc tatgcccggc cgtcgctcgt ccccgccaat
     4141 ctgaaaaacg cgccgcgcgt cgcgctgctg gtcggcgggc tcggcctcag cgaggaggga
     4201 accagccagg cgatccgaca attgccgggc gcagttacgc tcggcttcgc gccttacggc
     4261 gccgccgtcg aggctgaggt cgccgaggcg cgcgaagcag gccacgaggc gatcctgcaa
     4321 atcccgatgg agcccttcga ctattccgcc aacaatcccg gcccgcatac tctgctgacg
     4381 tcgaccggcg agcggcagac ccttgacgac cttcactggc tgatggcgcg cttccagggc
     4441 tatgtcggga tcgccaattt cctcggcgcc aaattcaccg ccgacgaggc cgcgctatcg
     4501 ccggtcctgc gcgacatagc cggacgcggc ctgctttatg tcgacgacgg ctcctcgccg
     4561 cgcagcctcg cccgcgctct cgccggccgc ctcgatcttc ccgcggtgac cgccgatgtg
     4621 gtgatcgacg ccgtgccgac gccgcaggcg atcgacgcgg cgctgacccg gctcgaagcc
     4681 ttggcgcggg ccaatggctc ggctttgggc gttgcgtcgg ctttgccgac gagcgtcgag
     4741 catatttcgc gctgggcgcg cgcgctcgaa gggcgcagcc ttgctttgac gccggtctcg
     4801 gcgatagtga cccgcgcgcc tggcccggcc gccgagaacg ccgcgccggc cctgccgcgg
     4861 tgagccgatg aggttcgagg atctgcccta tcgtccgagc gtcggcgtcg cgctgttcaa
     4921 tcgggaggga atggtttttc tcggccgacg caagaagcgg cgggtcgcca gcaaggattc
     4981 gatcggccac gaatggcaga tgccgcaggg cggcatcgac aaaggcgaga cgccgcgcgc
     5041 cgcggcgctg cgcgagctct acgaggagac gaatgtcgag agcgtctcgc cgctcgccga
     5101 agcgccgcaa tggctgacct atgacctccc gcccgacgtc gcggccaatc gctggcgcgg
     5161 ccgctttcgc gggcagcggc agaaatggtt cgccttccgc ttcgaagggg aggacagcga
     5221 aatcgacatc gaccggccgg cgaaaggcgc ccatccgccg gaatttgacg cctggcggtg
     5281 ggagcggatc gaggagctgc cggcgctggt gatcccgttc aagcggaacg tctacgaggt
     5341 cgtggtcgca acattcgcgc cgctcgtcaa gccggccggc tgacgcaatc gtacctcgcg
     5401 tgcgtggaat tcacagatcg gctgacacgg cccatcattg caagcgaagc gaagcaatca
     5461 agcgccgccc tgcgaatgga ttgcttggtc gcttcgctcc tcgcgaatga cagcgttccg
     5521 tcatcattga caaccctcgc ctcggccgcc ttggtctggc gctccggcgc cgctcgattg
     5581 cgctttgccg gaacgcgccg taacagggga gatatgcccg cgcccttcaa caagctcctg
     5641 cgcgaaatcc gcgcctgccg tgtctgccgc gacgcgccgc gctccggccg ccctttgccg
     5701 catgagccgc ggccgattat ccaagcttcg atcgaggcgc ggctctgcat cgccggccag
     5761 gcgcccggcg tacgcgtcaa cgcatcgggc cgcccgtacg acgacccgtc cggcgtgcgc
     5821 ctgcggcaat ggctcggaat cgacgaagcg acgttctacg atcctaaaaa gatcgccgtc
     5881 gtaccgatgg gattctgctt tcccggtctc gacgcgaagg gcggcgactt gccgccgcgc
     5941 aaagaatgcg ccgcgacgtg gcgcaggcgc attttcgacg ccctgccgag gatcgagctc
     6001 attcttctcg ttggccttta tgcgcaaaag tggcatctcg gcgcggcggc gaccgcggag
     6061 ggcctgtcgg gtacggtcgg caaatggcgc gacatttatc gcccgaaaga ccagcgccgc
     6121 cttttgccgc tgccccatcc ttcctggcgc aacaatgtct ggctcaagcg aaacccctgg
     6181 ttcgaggcgg agctcctgcc ggtcctgcgc ggcgaagtca gccggctgac ggcgtgacgg
     6241 cgctacccgg cggcgatctc gcgacgcgcc tgggcgagcg cctcgctcat cttgcgccga
     6301 atgcggtgat ccgaaatatc gacgccggcg gcgctcaaat cggcgcgcaa ggttttgaac
     6361 agcgcctcgt catccgcgct ctcgacttgc gccgcgacca gcgccttggc gtagctttcc
     6421 gcctccgcac cttgtttgcc cgtcaattgc gccgcccata ggccgaggag ccggttgcgt
     6481 cgcgcgcgcg ccttgaagct caaggcctcg tcggcggcga agcgcttctc aaacccttcc
     6541 tcccgctctt cgaacgcgcc catcgcccat cctcctgcgc aaagaccatg acctgaaacg
     6601 ggcgcgatgg gcaaccggtt cggcctgaaa cgtagaggct ccccggattc ttgccttcag
     6661 cgggaatgac cgcagcgcgt cttcaccggc gacgatcgag gcaggctcgc gccaagtcct
     6721 gaaatgcgcg cgcgcgatgc gatagcgcgc gcgagccgtc ggcgggaatg ccgtgctttt
     6781 cctcggcggt catctcgccg aagccgcgcg cgtggccgtc gggtacgaag atcgggtcgt
     6841 agccgaagcc gtggacgccc cgcggcggaa agaccaggcg ccc
//
LOCUS       DATGJW010000323         2003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_683100, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000323 DATGJW010000000
VERSION     DATGJW010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2003
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_683100"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..478)
                     /gene="prs"
                     /locus_tag="VKU03_09880"
     CDS             complement(<1..478)
                     /gene="prs"
                     /locus_tag="VKU03_09880"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HLJ71616.1"
                     /translation="MKILAGNSNPALAEAIAAYLGEPLTKLHARRFADLEIFVEILEN
                     VRGRDTFIIQSTSYPTNDNLMELLIMIDALRRASAQRITAVIPYFGYARQDRKPGPRS
                     PISAKLVANLITRAGADRVLTMDLHSGQIQGFFDIPTDNLQAAPLMADDIKAHYRDC"
     gene            complement(752..1342)
                     /locus_tag="VKU03_09885"
     CDS             complement(752..1342)
                     /locus_tag="VKU03_09885"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71617.1"
                     /translation="MISICQRWPGAAFVVLAAIGLGGCVETTAELAPGADAQHLHLAR
                     RPGVSLAGATVAFVSVDGPPADVSANFMKELARDAAAQDIVVADAKKARYFVRGYLSA
                     YPTQDGAAVEYVWDIFTKDRARAQRLSDYLVVKGEGSDPWAVAGAGALASVAAKSADD
                     LAAFLSNTPEAVAGATIAPGTTAAVEKASGALAYAK"
     gene            complement(1404..>2003)
                     /locus_tag="VKU03_09890"
     CDS             complement(1404..>2003)
                     /locus_tag="VKU03_09890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963949.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HLJ71618.1"
                     /translation="AILASMHNAIFAAGGDYPANEFIIGSAGDALLCRYKAGRRRLDA
                     EDQITLEFAGVYRHYHATIMRTHIVGAPRPLHRAYHEAARAALVACEAELRPGRTAGD
                     VYAAHARIFDAHGLSRHRLNACGYSLGAKFTPSWMDPPMFYEGADFVIGEGQVYFAHM
                     ILMDSESETALCLGRTYLIGPAGAAPLNAASLDLTVKSG"
BASE COUNT          332 a          656 c          694 g          321 t
ORIGIN      
        1 cgcagtcgcg atagtgggcc ttgatgtcgt cggccatcag cggcgcggcc tgcagattgt
       61 cggtcgggat gtcaaaaaag ccctgaatct gacccgagtg caggtccatg gtcagcaccc
      121 ggtcggcgcc ggcccgggtg atcagattgg cgacgagctt ggccgagatc ggcgagcgcg
      181 ggccgggctt ccggtcctga cgggcatagc cgaaataggg aatgaccgcg gtgatgcgct
      241 gcgccgacgc gcgcctcagc gcgtcgatca tgatcagcag ctccatgaga ttgtcgttgg
      301 tcggatagga cgtcgactgg atgatgaacg tgtcgcggcc gcgcacattt tcgaggattt
      361 cgacgaaaat ttcgagatcg gcgaagcgcc gcgcatgcag cttggtcagc ggctcgccga
      421 gataggcggc gatcgcctcg gccagcgccg gattggagtt gcccgccagt attttcatcg
      481 ccccgatcat cgccatcccc ttcgcgcggc gcgggtctta gcagccgctc cggccgggaa
      541 caacatggcg cgcggcgctc tcgatgctga agctcaccct caacccaacc ctctcccgca
      601 aacgacccgc aagcgggaga ggggcgggcg gcgcggtctt catcggcgac gagacattgg
      661 tctgcgatga tgagcggccc cggagggccg gatgagattc gccgcggctg cgctgcagcc
      721 gcggtcgccg acagcggcta caacccgtcg cttacttggc gtaggcgaga gccccgctcg
      781 ccttctcgac cgccgcggtc gttccgggcg cgatcgttgc gccggccacc gcttccggcg
      841 tgttggagag aaacgccgcc aaatcgtcgg cgctcttggc ggcgacgctc gccagggcgc
      901 cagcgccggc gaccgcccag ggatcgctcc cctcgccttt gaccacgaga tagtcgctga
      961 gccgctgcgc ccgcgcccgg tccttggtga agatgtccca gacatattcg acggcggcgc
     1021 cgtcctgcgt cggataagcc gagaggtagc cgcgcacgaa atagcgcgcc ttcttggcgt
     1081 cggcgacgac aatatcctgc gcggccgcgt cgcgggccaa ttccttcatg aaattcgccg
     1141 agacgtcggc cggcggcccg tcgaccgaga cgaacgcgac cgtcgctccg gcgaggctga
     1201 cgccgggccg gcgcgcgaga tggagatgct gcgcgtcggc gcccggcgca agctcggccg
     1261 tcgtctcgac gcagccgcca agaccgatgg ccgccagcac gacgaacgcc gcccccggcc
     1321 agcgttggca gattgaaatc attgacgctt tcgcctcccg ctgcggggtt tcatccccgg
     1381 cggatcgtta tcgaggagaa ctgctagccc gatttgacgg taaggtccag gctggcggcg
     1441 ttgagcgggg ccgcgccggc cgggccgatc aggtaggtgc ggccgaggca gagggcggtc
     1501 tcgctctcgc tgtccatcag gatcatatgg gcgaaataga cttggccctc gccgatgacg
     1561 aaatcggcgc cctcgtagaa catcggcggg tccatccagg atggcgtgaa tttcgccccg
     1621 agcgaatagc cgcaggcgtt gaggcgatgg cgcgacaagc catgcgcgtc gaaaatgcgc
     1681 gcatgggcgg cgtagacgtc gcccgccgtg cggccgggcc tcagctccgc ctcgcaggcg
     1741 acgagcgcgg ccctggccgc ctcgtgatag gcgcgatgca gcggccgcgg cgcgccgaca
     1801 atgtgggtgc gcatgatcgt cgcgtgataa tggcggtaga cgccggcgaa ttcgagcgtg
     1861 atctgatcct cggcgtcgag cctgcggcgc cccgccttgt agcggcaaag gagcgcgtcg
     1921 ccggccgaac cgatgatgaa ctcattggcc ggatagtcgc cgccggccgc gaaaatcgca
     1981 ttgtgcatcg aagccaggat cgc
//
LOCUS       DATGJW010000324        21660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1306689, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000324 DATGJW010000000
VERSION     DATGJW010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 21660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21660
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1306689"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VKU03_09895"
     CDS             complement(<1..534)
                     /locus_tag="VKU03_09895"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ71619.1"
                     /translation="MGRKHLLTTAAALALAALTADGASAAGKYVVGVSNTLIGNGWRE
                     EMICSIKAEAEASGLVSKVIVANRNGGPSEQIADIRNLISAGANAIIINPSDREALNP
                     VIKQAADKGIVVVAVDQAVSAPEAYVLTNDQVDYAKLGAKWLFDKLGGKGNVIEMRGI
                     DGVPADADRHNGFEQVLK"
     gene            complement(596..1780)
                     /locus_tag="VKU03_09900"
     CDS             complement(596..1780)
                     /locus_tag="VKU03_09900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ71620.1"
                     /translation="MVYATADGGGARKLRIGMVGGGRTAFIGAVHRMAARLDDLIVLT
                     AGALSADPENARLSGADLGLDPDRVYSDYREMAAREAARPDGIEAVVIVTPNHLHHPV
                     SKAFLEAGIDVICDKPLATSLEEARELIALTRAKRLVFAVTLNNTGYPMVRQAREMIK
                     AGEIGDIRVVHAAYIQDWLTEPIDAEGQKQAEWRTDPARAGASACLADIGVHAHNLAL
                     FVTGLELDEVSADLATFAPGRRLDDNAHVLMRFAGGARGVLASSQVAVGNLNNLSLKV
                     FGAKGGLEWAGETPEFLRFTPYGQPTRILQRGGPANSAEAKRGSRMPAGHPEGYVEGF
                     ANLYRDAAELISARRAGRPPNPAALAATPDVVDGARGLAFIEAAVASSRKNGAWTSAR
                     FV"
     gene            complement(1785..2843)
                     /locus_tag="VKU03_09905"
     CDS             complement(1785..2843)
                     /locus_tag="VKU03_09905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLJ71621.1"
                     /translation="MRTIKGPGLFLAQFAQDTPPHNTLEGIARWAKGYGYKAIQIPTW
                     DRRFFDLDRAFDSEAYCDEIKGKLADIGIAVSELSTHFQGQMVSVHPAYDALFDGQCP
                     AAVRGNPEKRRLWAAEQVRKGAKVSRRFGLTEHVTFTGSLAWPFIYPYPQRPAGLIEE
                     CFAEQARRWKPILDLYDENGVDLCFEIHPTEDVFDGDSFDMFLDAVGGHKRCNINYDA
                     SHYIKQGMDYLGFIDAYHDRIKMFHVKDAEFNPTAKQGVYGGYKGWLERAGRDRSLGD
                     GQVNFRAVFSKLAAYDFAGWAVYEWECCLQHPEVAARKGAQFIADHIIEVTDRVFDDF
                     AATGADRATNLKLLGVKG"
     gene            3140..3925
                     /locus_tag="VKU03_09910"
     CDS             3140..3925
                     /locus_tag="VKU03_09910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ71622.1"
                     /translation="MEARLDGRIVLVTGATQGIGRAIAAAAAKAGAAGLALTGRDAPR
                     GASAAAEISALGAPALFFAADLGEPQAPNRILSAALERFGRVDALINAAALTDRGAVA
                     DASPDLWDKLYAVNARAPFFLMQGVVNHLRARRAPGAIVNILSINAHGGTPALAVYAS
                     TKASLALLTKNAAYAHRFDRIRVNGINVGWADTPGERHMQAVTLGLGEAWLAAANAAQ
                     PTGKLLSADDVARLALFLLSDLSAPMTGALIDQDQSNVIGVRD"
     gene            3929..5416
                     /locus_tag="VKU03_09915"
     CDS             3929..5416
                     /locus_tag="VKU03_09915"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HLJ71623.1"
                     /translation="MERLRQSALPSLNPAIRRPQYDRDKLAVGMAHIGVGAFHRCHQA
                     EFTDDALEAEFGPWGVLGINLRPPRLAGLLSPQDGLFTRTLREGERAETRVIGAIRQV
                     VDVEDAVSAERAIAALASPAIGVVTMTVTEKGYCHVPATGALDWANPALRHDRGGAAA
                     PATIFGLLTRALEKRRATGAGGLTIISCDNLPSNGALLRGALAAFIRAGAPALADWTE
                     KHVAFPSTMVDRIAPAPSEADVVYASDALGAVDEAAVVGEPFRQWAIERAFSGDPPPW
                     ERAGAQFVADVKPYELIKMRVLNAAQSTLSHLGALLGLTYSFEAAGDPVLAALVRRTL
                     ETETASTLPDVAGMPVRAYINTSLQRIANSAIRHRCHQIGTDGSQKIVQRLVNPLRER
                     LAAGHRPGLLALAVASWMAYCLSGARRFGARWRPDDPWAGRVIALGEENGDDFRALAK
                     AMLGIEAIFGNDLAASLAAEAIAAHLEGLLSGDPRLYLTRRLSHG"
     gene            5433..6368
                     /locus_tag="VKU03_09920"
     CDS             5433..6368
                     /locus_tag="VKU03_09920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HLJ71624.1"
                     /translation="MAEIAPSIDKKQDRAALGYSRPESYEELRAVLSSGTMRLPTKLR
                     QVAIYLWQHPTVVALGNVTEVARQAGVQPSTLVRFAQTFGYTGFSDLQEVFKTYLSGG
                     WRDKSASGSDSGGESARLIDGFVKSSTASLSRVRDRLDMIGFEAVAEALAKAELIYIL
                     GSKRAFTVSSYMSLALSKLGVRNIAVDNVGAAAFEHLRCATETDAVLAISFTPYNSIT
                     PELAASAAERGVPMVSITDSAFSPLVPLSKAYIEVVEEDFSGFKSLAATLAVAMALVL
                     RVAQLRGASAEPGGDGKPRRNARRQSAGRKPALSP"
     gene            6396..7472
                     /locus_tag="VKU03_09925"
     CDS             6396..7472
                     /locus_tag="VKU03_09925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018429499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ71625.1"
                     /translation="MKTWRVVGISFEHMHMGDLLRQAHEHPKAEIAGICDPDPKRMAA
                     AVSTFSIPPDRVFTDIRACIEKTRPDLAILCPATARHAEVAEAVAALGVDMLIEKPFA
                     ASLADADRILAAIAKSGVRLAINWPLRWYPPHVTAKRLIDEGAIGEVTEVHFYDGNRG
                     PLYHLADKIEVSEEDVRRQKPSSWWYKKVAGGGSLLDYLGYGATLGTWYMNGAAPIEV
                     TSVVDQPEGLEVDEHSITVCRYKSGLSKMETRWGAFTDPWTLQPQPKCGFVIVGTDGT
                     ISSYDYEPHVGVQTRTRKEIHYLPADKLEPPFRAPIEYVLHCKETGTPFEGPLDPALC
                     RTAQRIVDTAALSAKERRTLALLP"
     gene            7469..8575
                     /locus_tag="VKU03_09930"
     CDS             7469..8575
                     /locus_tag="VKU03_09930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011976168.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ71626.1"
                     /translation="MSEPDADPAERDYALVGKKAAEIAAPDLPYRPPMPKDRTIGIGL
                     IGAGGISAAHLDAYRAYGLNVVAIASRDLGRAKARRDAYFPEASATDDVGRLLRDPAI
                     KALDITTHPEPRVELMRRAIAAGKHVLSQKPFVVDLATGAALVEEAARAGVKLAVNQN
                     GRWAPHLSYMREAVQAGLIGAVTGAHVAIHWSHGWIAGTPFDEIDDAILWDFGIHWFD
                     FLVSLIGEQATSVIASRAFASGQTARPPLLAQALVAFPGGQASLVFDGDTKYGALDTT
                     IVVGSAGSVSSAGPNLGEQSVVLATAAGVARPKLQGTWFNDGFAGAMGELLSAIEDDR
                     EPLNSARGNLASIRLCQAAIRAAHRGERIVLRPD"
     gene            complement(8701..9099)
                     /locus_tag="VKU03_09935"
     CDS             complement(8701..9099)
                     /locus_tag="VKU03_09935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71627.1"
                     /translation="MMTFNHVANRLDHRHFETIVEMLKAKLGFFELRRTERAIWLRQK
                     GANIDLQFSRSASLGRDEDKIRSQVSFLSETPRADLEELAAWARTHGLKAEVGSYSDR
                     EFYLDAPEAFVDFVIEAMRPELADYGAVSP"
     gene            9252..10076
                     /locus_tag="VKU03_09940"
     CDS             9252..10076
                     /locus_tag="VKU03_09940"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ71628.1"
                     /translation="MQAFRRDNRERPALDRHLIADLSEKIRAAGSPRDVLVKRVADAL
                     RGEIASGQLKPGARLISEVALAQNLQISRPTLREAMRILAREGLLVIKHGVGAFVADE
                     HRLIWGRLDAMRSFTDLIRSVGGEPGDSHLSIERATAADEVAEALNIPPQTPVGLVKR
                     VRLIDGAPLAIANEYVLLPKPEDDFARLKTFDGGSLYAFLHERCGVTLARSSVVITAI
                     PADAARAKLLKVPRRTPLLLLREPHYDVSGRPVLFAVNYHNSNLVQFTLTRAGLRT"
     gene            10073..10987
                     /locus_tag="VKU03_09945"
     CDS             10073..10987
                     /locus_tag="VKU03_09945"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLJ71629.1"
                     /translation="MSGGCFFALGNISIDDLVFADGATMWAVPGGNAIYSALGMAVWG
                     ERANVVAPIGPEYPVEKLGDRIDLSRCRPIARTLRDWGLYEEDGTREFVFRSNTRNWI
                     DFCPSVSDLGPGPYPFCHLAPLPWRLHIELAATLRERGAELISADPDDRRLHEIQRAE
                     IAQLLRLVDIFLPSRQDAAAMFPGLSPLDALKALRALSPETPAIVVKCGAAGAIAHQR
                     DDRDYLEIPSAAARAVDETGAGDAFCGGALAGYARRRELAAALAHAAVSASFAVEATG
                     SAALVASASPTAEERLARISRRIETRRL"
     gene            11031..12182
                     /locus_tag="VKU03_09950"
     CDS             11031..12182
                     /locus_tag="VKU03_09950"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HLJ71630.1"
                     /translation="MDEEKRVMLQEIAMQPDFVRDNLDPMLDATRESLAEHKGREISA
                     GFMIGCGDSYCAALAARGFMMDATGKMAEPVEALEFSRYLVSYIPRDAFVFGVSNSGT
                     VSRTIEGIRLARERGAWTFAVTVSATNRLAEAAETLIKVNATPNIKERPGGTRIVTPG
                     TVTYTASMLGLFVAAIALGEKIGHLDKSRATTLIDQIRRAADAMAAADEMSRTIAGDI
                     AATFLPSRKTVILGGGPNYATAYFAMAKWHEALTRPCHASELEEWAHEEYFHTDENSD
                     TFVLLPPGGGHSRGLEQALAAKEMGSRVLIIGAADDEAARKAADVYFAMPADVPESLT
                     PLVYKAPFEHLSCQIAHEQKIPFLGFHNPKRQQVNFRQIFSSAQTTATG"
     gene            12187..12996
                     /locus_tag="VKU03_09955"
     CDS             12187..12996
                     /locus_tag="VKU03_09955"
                     /inference="COORDINATES: protein motif:HMM:TIGR00259.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BtpA/SgcQ family protein"
                     /protein_id="HLJ71631.1"
                     /translation="MRKLLSLIQAGGKPIIGMVQLGALPGGSRYRGGAIGEIVQAALD
                     DATLLARHGVDALMVQNLGDLPVDARVSSAQLAWMTRVATEIRSRFDGPLGLNFLEND
                     AEAMLAVASAAGADFVRIKVYVGAMMTPFGIESGQAFAAIRARNALDAAGVAVFADVH
                     DRTGTPIASGGFEEDLDYATRLGGADGLVLTGKTYAQTLEFLRGARERVGRTPILVGG
                     GVSEKNFAEVAKLADGVIVSSSLKDTSRAFGAFVPAKVESFMNEARRVRSM"
     gene            13044..14099
                     /locus_tag="VKU03_09960"
     CDS             13044..14099
                     /locus_tag="VKU03_09960"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLJ71632.1"
                     /translation="MLKLLAKFSALAVMTASLAFSTAAEAKLKVAFVTSESGLGDRSF
                     NDMMNQGMKRAVKELGIDYVIIQPRSISEFQSTLARAAGQGFDIIIGSSFDMIKPMKA
                     VAAAFPQQKFGLVDVGPDPIAPNVVSSVTKDWEGSFLVGVIAAKVTKTGTIGFVGGKD
                     IPIIHRFFIGYYYGAKMAKPETKVLESYSGTFTDPAAGKEYTLALVNQGSDINFAVAG
                     ATSAGVIDAAKSSNTFAIGVDSDQNYMAPGHVLTSMVKRVDTQAFDMIKSVADGTFKG
                     GTVLYYGLKEGGVDAAMDQYNKGLIPDDVLKQVDDLRQKVISGEIVVPNYFDLKPGQK
                     EMGKPPIATPPSIANAK"
     gene            14155..15717
                     /locus_tag="VKU03_09965"
     CDS             14155..15717
                     /locus_tag="VKU03_09965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71633.1"
                     /translation="MEAGVDLVAMTHIAKRFEALTAIHDASLTIRPGEIHALVGENGA
                     GKTTLMNILYGLLPRDGGEILLRGAAANFSSPAEAIAAGIGMVHQHFKLAPSFTVTEN
                     IILGAEPLRGFNRINRGDAERQTAELGARFGLDLDPRAVVGDLPVGLRQRVEILKALY
                     RDAEILILDEPTAVLTPQETRELFATMRRLAESGRAIIFITHKLREVLAVSDRVSVMR
                     LGRIISTEPNKNLTAERLASLMVGRTVLLRVSKAKASPSEEKLLQVEGLTAIGDRGET
                     AVDNLSLAVRAGEIVGLAGVQGNGQDELVQCIAGLRRPLSGAVAICGRAADRWEPSGC
                     RDAGLAYIPADRGRVGLSLASEVWENLSLGHLPSFSRGPILLTEKARRQAGALIRKFD
                     VRGASEATLAKSLSGGNQQKLQLARELTRGARLVVAEQPSQGVDIGAIEAIHRILVDM
                     RDQGRAVFVISADIDEIFSLSDRILVIYRGRIVASLAADEADAETIGRFMGGLHDQPD
                     IEASEHGRAHVQ"
     gene            15695..16837
                     /locus_tag="VKU03_09970"
     CDS             15695..16837
                     /locus_tag="VKU03_09970"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71634.1"
                     /translation="MDELMSSEAAPLRRSAISLRGLVRPLAGPAVAVLIAIVVGALLV
                     AAVGQNPLDVYVTLIKGGLVGWPNLSVALQLTTPLIFTGLAVAISFRAGLWNIGAEGQ
                     MLMGALAAGIVGYAVSLPVYLHIPLCVIAAFLGGAAWAGIPALLRVYLGVNELVVCLM
                     MNPIALLLTGYVSARALKAPGPTNKLPDILETAILPNFSLFSQLNAGIFIALACCAIV
                     AFVNSATLRGFEWKMIGLNPRFAYYGGIDVRKSALTIFLLSGGIAGLAGAEQVLGVYK
                     AYYDNFSPGYGFDGIAVAMLAKNNPIGVILAAFLFGALNGGSAVLQMMTGVSKYLVQV
                     LQFIIVLVLAAQFSFRWRRATTGLRSSAAPKGGSQGLGKPPLSESE"
     gene            17043..17960
                     /locus_tag="VKU03_09975"
     CDS             17043..17960
                     /locus_tag="VKU03_09975"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71635.1"
                     /translation="MISAIAFSAIRISTPLIYAALGGLLTYQAGILNIALDGFMIVAA
                     FSAIAVAYATESLTLGICAGVASSVVLAALLALFNLRFKANIFIAGIAVTFMAYGLTA
                     LLLKGSLGQEGVFSSDRIPTFPPLHIPLIADIPILGPLVSGHTLLVYFAYLLVPVVAF
                     FLYRTRWGLRVRVVGEAEEAAAAAGINVASLKFQTMLWSGVFCGLAGSYLSLGYVSLF
                     AKQMTNERGLIALAAIFFANGDPYWTAAVALLFGAATALSVQLPEMTGAAPQLLQLIP
                     YVVTVLALVIVGLRNSRRRNRYGVWRFEL"
     gene            complement(17969..19162)
                     /locus_tag="VKU03_09980"
     CDS             complement(17969..19162)
                     /locus_tag="VKU03_09980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLJ71636.1"
                     /translation="MTEKALSDITVLDFSHLLQGPFCTQLLADLGANVVKVERPGQGD
                     LFRTMTFNNRWMGGHESPNFLAWNRNKRSIAVDLKSPEAKTILYRIGAKADVVVQNFR
                     PGILARLGFGYEDFRRINPRIIYCSGSGYGESGPYVSRPGQDMLIQGLTGLAAATGRG
                     DGPPVPVGSGFSDQVGAMNMVYAILAALYWRERSGKGQEIKIDLLTAMLAHQGQEMLM
                     AMNFGKDFERPKSGIGHPGMEAPFGVYPTKDGWVTIAMSPYRTLVNVLGATELLAYDD
                     PESLFEERDEIWEKLAALTKSWATADLIEAMLKADIWCGEVKTHVEAADDPQVAHMRA
                     IGAYDHPAAGRVKVVAPAVKMSETPPAIERPAPRIGEHSAELLREFGFDQAAIDGFFA
                     SGAVA"
     gene            complement(19201..20076)
                     /locus_tag="VKU03_09985"
     CDS             complement(19201..20076)
                     /locus_tag="VKU03_09985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLJ71637.1"
                     /translation="MNHRFATAENHVLPMERARVFFDGLFGEPRLQHPEGVAIGPDGW
                     IWCGSENGEVLRIDPEGTRIERVAQGGGFTLGLAFLRDQALFFCDLRDKAVYRLDLAS
                     RRVERFTRPGIRIPNYPVVDAARSRLLVSDSHDFATPGPGVWAFDLATGEGKLWYEKS
                     LVFANGMALDASGGALFVCETFARQITRIAVAPDGSAGEAAPFAIELPGLPDGIAFDE
                     RGDLFCGCYEPSRILRIGKDGKAEVYIEDPTAHLFAHPTNIAFDGRALYTANLGRWHI
                     TRIESDTAARPLWSV"
     gene            complement(20117..20887)
                     /locus_tag="VKU03_09990"
     CDS             complement(20117..20887)
                     /locus_tag="VKU03_09990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877397.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71638.1"
                     /translation="MGDFEGRVAIVTGAAMGIGRACARLIAQGGAHVFIADIDAPAGE
                     ASAAEIAASGGSAHFLRVDVNALADLEAVAAAAVAKYGRIDILVNNAAQAIGGVVDET
                     DEATWNRVLSTNLSSVWRGMKACLPQMRKQKKGAIVNMSSVQALAGFKGWAAYATAKG
                     GINALTQQAAVELAPFGIRVNAVAPGTVMTPLNEKVFREHPNPDELIATWNRAHPIGR
                     FGQPEEVAELVAFLASDRASFITGEIMRVDGGLVVRGE"
     gene            complement(20889..>21660)
                     /locus_tag="VKU03_09995"
     CDS             complement(20889..>21660)
                     /locus_tag="VKU03_09995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="enoyl-CoA hydratase-related protein"
                     /protein_id="HLJ71639.1"
                     /translation="LLSETIEPGIVRLTFNRPEKLNALSSEVIRRLDEELDRIAADAS
                     ARVVILTGAGERAFVAGADIGEYRGRRAQAFIAYQHESRRVFDKLEALPKPTIAALRG
                     YALGGGFEIALCCDVILCAASARLGLPEGLLGLSPGGGGTQRLVRAVGKYVASDVMLA
                     ARRMTGERAFQLGLAAAVCPDEELAATAIERARAMLSVAPLAQAEMKRLVRLGPDAAL
                     PAAQTLEQEVLFRLYGTDDAEEGVAAFLDKRKPNFKGG"
BASE COUNT         3681 a         7244 c         6882 g         3853 t
ORIGIN      
        1 cttcagcacc tgctcgaagc cattatggcg atcggcgtcg gccggcacgc cgtcaatgcc
       61 gcgcatctcg atgacgttgc ctttgccgcc gagcttgtcg aacagccatt tggcgccgag
      121 cttggcgtag tcgacctggt cgttggtcag cacataggcc tcaggcgccg agaccgcctg
      181 gtcgaccgcg acgacgacga tgcccttatc cgcggcctgc ttgatgaccg ggttgagcgc
      241 ttcgcgatcc gacggattga tgatgatggc gttcgcgccg gcggaaatga gattgcggat
      301 atcggcgatc tgctcggacg gaccgccatt gcgattggcg acgatcactt tcgagaccag
      361 gccggaggcc tcggcctctg ccttgatcga gcagatcatc tcctcgcgcc agccattgcc
      421 gatcagcgtg ttggacacgc cgacaacgta cttgccggcg gccgaggcgc cgtcggccgt
      481 caaggcggct aaggccagcg ccgccgccgt cgtcaaaagg tgcttccgtc ccatgctttt
      541 tcctcccggg gtcatgcgcg ctccgacatg gtcacgagcg cgttcgttgc aaactctaca
      601 cgaaacgcgc cgaagtccag gcgccgtttt tccgacttga cgcgaccgcc gcctcgatga
      661 aggcgagacc gcgcgcgccg tcgaccacgt cgggcgtcgc agccaaggcg gcagggttgg
      721 gcgggcgtcc ggccctgcgc gccgaaatca actccgcggc gtcgcgataa agattggcga
      781 agccttcgac atagccctcg ggatgtccgg caggcatgcg cgagccgcgc ttcgcctctg
      841 cgctgttcgc cggtccgccg cgctgaagaa tgcgagtcgg ctggccgtaa ggcgtgaagc
      901 ggaggaattc cggcgtctcg ccggcccatt ccaggccgcc tttcgctcca aagaccttga
      961 ggctgagatt gttcagattg ccgaccgcca cctggctcga cgccagaacg ccgcgggcgc
     1021 cgccggcaaa acgcatcagc acatgggcgt tgtcgtcgag ccggcggccc ggcgcgaacg
     1081 tggccaaatc cgccgaaacc tcgtcgagct ccagccctgt gacgaacagc gccagattgt
     1141 gcgcgtgaac gccgatgtcg gcgagacagg ccgaggcgcc ggcgcgggcg ggatcggtcc
     1201 gccactcggc ctgtttctgg ccctcggcgt cgatcggctc cgtcagccag tcctggatat
     1261 aggccgcgtg cacgacgcgg atgtcgccga tctcgccggc tttgatcatt tcgcgcgcct
     1321 ggcgcaccat cggatagccg gtattgttca gcgtgacggc gaaaaccagg cgcttcgcgc
     1381 gggtgagcgc gatcagttcc ctcgcctctt caagacttgt cgcaagaggc ttgtcgcaaa
     1441 tcacgtcgat gccggcctca agaaaagctt tcgagaccgg gtgatgcaga tgattcggcg
     1501 tgacgatgac gacggcttcg attccgtccg gccgcgccgc ctcgcgcgcc gccatttcgc
     1561 gataatccga atagacgcga tcaggatcga ggccgagatc ggcgcccgag aggcgcgcat
     1621 tttcgggatc ggcggagagc gcgccagcgg tcaagacgat cagatcgtcg aggcgcgcgg
     1681 ccatgcggtg gacagcgccg ataaaggccg tgcggccgcc gccgaccatg ccgatcctga
     1741 gcttcctggc gccgccgccg tccgccgtcg cgtaaaccat tcgatcagcc cttgacgccg
     1801 agaagcttga gattggtcgc gcggtcggcg cccgtcgcgg cgaaatcgtc gaagacccgg
     1861 tccgtcacct cgatgatgtg gtcggcgatg aactgcgcgc ctttcctcgc cgcgacctcc
     1921 ggatgctgca gacagcattc ccattcatag acggcccagc cggcgaaatc gtaagcagcg
     1981 agcttcgaga acacggctct gaaattcact tggccgtcgc cgagcgagcg gtcgcgcccg
     2041 gcgcgctcga gccagccttt gtacccgcca tagacgcctt gcttggcggt cgggttgaat
     2101 tcggcgtcct tgacgtgaaa cattttgatg cggtcgtgat aggcgtcgat gaagccgaga
     2161 tagtccattc cctgcttgat gtagtggctc gcatcgtaat tgatgttgca gcgcttgtgg
     2221 ccgccgaccg cgtcgaggaa catgtcgaaa ctgtcgccgt cgaaaacatc ctcggtcgga
     2281 tgaatctcga agcaaaggtc gacgccattc tcgtcataga gatcgaggat cggcttccac
     2341 cggcgcgcct gctcggcgaa acattcctcg atcaagccgg ccggccgttg cgggtaagga
     2401 tagatgaacg gccaggcgag cgagccggtg aacgtcacat gctcggtcaa gccgaagcga
     2461 cgcgatactt tggcgccttt gcgtacctgc tcggccgccc atagccggcg cttttccgga
     2521 ttgccgcgga ccgcggccgg gcactggccg tcgaacagcg cgtcataggc cggatgaacc
     2581 gacaccatct ggccctggaa atgcgtggag agctcgctga ccgcgatgcc gatatcggcc
     2641 agcttgccct tgatctcatc gcaataggcc tcgctgtcaa aggcgcggtc gagatcgaaa
     2701 aagcggcggt cccaggttgg aatctgaata gccttgtagc catagccctt ggcccagcgc
     2761 gcgattccct cgagcgtatt gtgaggcggc gtatcctgcg cgaattgagc gagaaacagg
     2821 cccgggccct tgatcgtccg catgaggcgc ttccttccct cgccgcgctc gcgaatgggc
     2881 gcggcctatt tcgcggctga aaaaattcag cgcttgaatc gataatcgca ataattttcc
     2941 atttgtcaac ttaacgaccg cgccgccgat caaagccggc attgtgggaa aaatcatact
     3001 gaaataccgt ttctttcaaa gccggcgcgg tttcgcgccg gaagcggctc gatacgcaac
     3061 atgcagaata ttttttctca agattgactt ttggaacatc tcattcacaa tcggcgaaac
     3121 gaaacaaaac ggtttgccga tggaagcgcg cttggatggg cggatcgtcc tcgtcaccgg
     3181 ggcgacgcag ggaattggcc gcgcgatcgc ggccgccgcg gcgaaagccg gcgcggccgg
     3241 cttggcgctg accggccgcg acgcccctcg cggcgcgtcg gccgcggccg aaatttcggc
     3301 gctcggcgcg ccggcgttgt tcttcgccgc cgatctcggc gagccccagg cgccgaacag
     3361 aattctgagc gccgcgctcg aacgcttcgg ccgcgtcgat gcgctgatca acgccgccgc
     3421 gttgaccgat cgcggcgcgg tcgccgacgc ctcgcccgac ctttgggaca agctctacgc
     3481 ggtgaacgcc cgcgcgccat tttttctcat gcagggcgtc gtcaatcatc tgcgcgcgcg
     3541 ccgcgcgccc ggcgcgatcg tcaacattct gtcgatcaac gcgcatggcg gcacgcccgc
     3601 cctcgccgtc tacgcttcga ccaaggcgtc gctcgctctc cttaccaaga acgctgctta
     3661 cgcccatcgc ttcgaccgca tccgcgtcaa cgggatcaat gtcggctggg ccgacacgcc
     3721 gggcgagcgc cacatgcagg cggtgacgct cggccttggc gaagcctggc tggcggcggc
     3781 caacgcggcg cagccgaccg gcaagctttt atcagcagac gatgtcgccc gccttgcttt
     3841 gttccttctc agcgatcttt ccgcgccgat gaccggcgcg ctgatcgatc aggatcaatc
     3901 gaacgtcatc ggcgtcaggg actgaccgat ggagcgcttg cgccaatccg cgctgccgag
     3961 cctgaatccc gccattcgcc gcccgcaata cgatcgcgac aagctggcgg tcggcatggc
     4021 gcatatcggc gtcggcgcct ttcatcgctg ccaccaggcg gaatttaccg acgatgcgct
     4081 cgaagccgag ttcggcccct ggggcgttct cggaatcaat ctgcgcccgc cgcgcctcgc
     4141 cggtctgctt tcgccgcaag acggcctgtt cacgcgcaca ttgcgcgagg gtgagcgcgc
     4201 cgaaacgcgg gtcatcggcg cgatccggca ggtcgtcgac gtcgaggatg cggtgagcgc
     4261 cgagcgggcg atcgccgcgc tggcgtcgcc ggcgatcggc gtcgtcacga tgaccgtgac
     4321 cgagaagggc tattgccacg tccccgcgac cggcgcgctc gattgggcca atcccgcgct
     4381 tcgccacgac cgcggcggcg ccgccgcgcc ggcgacgatt ttcggattgt tgacgcgcgc
     4441 gctcgaaaag cgtcgcgcga cgggcgcggg cggcttgacg atcatcagtt gcgacaacct
     4501 gccgtccaac ggcgcgctgc tgcgcggcgc gctcgccgcc ttcattcgcg ccggcgcgcc
     4561 ggcgctggcg gattggactg aaaagcatgt cgcctttccc tcgaccatgg tcgatcgcat
     4621 cgcgccggcg cccagcgagg cggatgtcgt ctatgcgtcg gacgcgctcg gcgcggtcga
     4681 cgaagccgcg gtggtcggcg agcccttccg ccaatgggcg attgagcgcg cctttagcgg
     4741 cgatccgccg ccttgggagc gcgccggcgc gcaattcgtc gccgacgtga agccctacga
     4801 gttgatcaaa atgcgggttt tgaacgccgc gcaatcgacg ctgtcgcatc tcggcgcgct
     4861 gctcggcctg acttacagtt tcgaagcagc cggcgatccc gttctcgcgg cgctggtccg
     4921 gcgaacgctc gaaaccgaga cggcctcgac ccttcccgat gtcgccggca tgccggtgcg
     4981 cgcctatatc aatacgtcgc tccagcgcat cgcgaattcc gcgatccgcc atcgctgcca
     5041 ccagatcgga accgacggct cgcagaaaat cgtgcagcgc ctggtcaatc cgctgcgcga
     5101 gcgcctcgcg gcgggccatc gtcccggcct gctcgccttg gcggtcgcga gctggatggc
     5161 ctattgcctt agcggcgcgc ggcgcttcgg cgcccgctgg cgccccgacg atccgtgggc
     5221 cggccgcgtc atcgcgctgg gcgaggagaa cggggacgat ttcagggcgc tcgccaaggc
     5281 tatgctcggc attgaagcga tctttggcaa cgatctggcc gcttcgctgg cggccgaggc
     5341 gatcgctgcg catttagagg gccttctgtc cggcgatccg cggctttatc ttacccgtcg
     5401 cctgagccat ggctgaggga gccgagccgc ctatggccga gatcgcgccg agcatcgaca
     5461 aaaagcagga tcgcgcggcg ctcggctatt cgcggccgga aagctacgaa gaacttcgcg
     5521 cggtgctttc ctccggcacg atgcgcctgc cgacgaaatt gcgccaagtc gcgatctatc
     5581 tatggcaaca tccgaccgtg gtcgcgctcg gcaacgtcac cgaggtggcg cgccaggcgg
     5641 gggtgcagcc ctcgaccctc gtccgtttcg cccaaacctt cggctatacc ggcttttccg
     5701 acttgcagga ggtgttcaag acctatctca gcggcggctg gcgcgacaaa agcgcgagcg
     5761 gaagcgacag cggcggcgaa tcggcgcggc tgatcgacgg cttcgtgaag tcttccacgg
     5821 catcgctatc ccgcgtccgc gaccgcctcg acatgatcgg cttcgaagcc gtcgccgagg
     5881 cgctggctaa agccgagctc atctacattc tcggatcaaa gcgcgccttc accgtttcga
     5941 gctatatgtc gctggcgctg tcgaaactcg gcgtccgcaa catcgcggtc gacaatgtcg
     6001 gggcggccgc tttcgagcat ttgcgctgcg cgaccgagac cgacgcggtt ctcgcgatca
     6061 gcttcacgcc ctataattcg atcacgccgg aactcgccgc ctccgcggcc gagcgcggcg
     6121 tgccgatggt ctcgatcacc gacagcgcct tcagcccgct ggttccgttg tccaaggcgt
     6181 atatcgaggt cgtcgaagag gacttctcgg gcttcaaatc gctcgccgcg acgctggcgg
     6241 ttgcgatggc tctggtgctg cgggtggcgc aattgcgcgg cgcttccgcc gagccaggcg
     6301 gcgacggcaa gccgcggcgg aacgcgcgga ggcaaagcgc tgggcgcaag ccggcgcttt
     6361 cgccatgacg ccaaatgaac aggagaaaaa gcgcgatgaa gacttggcgc gtcgtcggga
     6421 tcagcttcga acatatgcat atgggcgatc tgctccgcca ggcgcatgag catccgaagg
     6481 ccgagatcgc cggaatctgc gatccggacc cgaagcgcat ggccgccgcc gtctcgacgt
     6541 tctcaattcc gccggaccgc gtattcaccg atatccgcgc ctgcatcgaa aagacccggc
     6601 ccgatcttgc gatcctctgc ccggccaccg cccgccacgc cgaagtggcg gaagcggtcg
     6661 cggcgctcgg cgtcgacatg ctgatcgaaa agcccttcgc cgcttcgctc gccgacgccg
     6721 accgcatcct cgccgcaatc gccaaatcgg gcgttcgcct ggcgatcaat tggccgctcc
     6781 gctggtatcc gccgcatgtg acggcgaaac gattgatcga cgaaggcgcg atcggcgaag
     6841 tgaccgaagt gcatttctac gacggcaatc gcggcccgct ctatcacctc gccgacaaga
     6901 tcgaggtcag cgaggaggac gtccggcgcc agaagccctc gtcctggtgg tacaagaagg
     6961 tggccggcgg cggcagcctg ctcgattatc tcggctatgg cgcgacgctc ggcacgtggt
     7021 acatgaacgg cgctgcgccg atcgaggtca cgtccgtcgt cgaccagccg gaagggctcg
     7081 aagtcgacga gcattcgatc acggtctgcc gctataagtc aggcctttcg aaaatggaga
     7141 cgcgctgggg cgcgttcacc gatccctgga ctttgcagcc gcagccgaaa tgcggcttcg
     7201 taatcgtcgg gacggacggc acgatttcga gctacgacta cgagccgcat gtcggcgtcc
     7261 agaccaggac gcgaaaggag atccactatc tcccggcaga caagctcgag ccgccgttcc
     7321 gcgcgccgat cgaatatgtc ctccattgca aggagaccgg aacgcccttc gaagggccgc
     7381 tcgacccggc gctttgtcgg accgctcagc gcatcgtcga caccgccgcc ttgtcggcga
     7441 aggagcgccg gactttggcg ttgttgccat gagcgagccg gacgccgatc cggccgagcg
     7501 cgactatgct ttggtcggca agaaggctgc cgaaatcgcc gcgcccgacc tgccctatag
     7561 gccgcccatg ccgaaggatc ggacgatcgg catcggcctc atcggggccg gcggcatcag
     7621 cgccgcccat ctcgacgcgt accgcgctta cggcctgaac gtcgtcgcga tcgcttcgcg
     7681 cgatttgggg cgcgccaagg cgcggcgcga cgcctacttt cccgaagcga gcgccaccga
     7741 cgatgtcggg cgcctgcttc gcgatccggc gatcaaggcg ctcgacatca cgacccatcc
     7801 ggagccgcgc gtcgaattga tgcgccgggc gatcgcggcc ggcaagcacg tcttgtcgca
     7861 gaagcccttc gtcgtcgatc tcgcgaccgg cgcggctttg gtcgaggagg cggcgcgagc
     7921 cggcgtcaag ctcgccgtca accagaacgg gcgctgggcg ccgcatctct cctatatgcg
     7981 cgaagcggtg caagccggcc tgatcggcgc cgtcaccggc gcgcatgtcg cgatccattg
     8041 gagccatggc tggatcgccg gcacgccctt cgacgaaatc gacgacgcga ttctttggga
     8101 tttcggcatt cactggttcg attttctcgt cagcctgatc ggcgagcaag cgacaagcgt
     8161 aattgcgagc cgcgccttcg ccagcggcca gacggcgcgg ccgcctttgc tcgcgcaggc
     8221 gcttgtcgcc ttccctggcg ggcaagcctc tttggttttt gacggcgata cgaaatacgg
     8281 cgccctcgat acgacaattg tggtgggctc ggctggctcg gtctccagcg ccggtccgaa
     8341 tctcggcgaa caatcggtcg tcctcgccac cgccgcgggc gtcgctcggc cgaagctgca
     8401 aggaacgtgg ttcaacgacg gcttcgccgg cgcgatgggc gagttgctgt cggcgatcga
     8461 ggacgatcgc gagccgctca attcggcgcg cggcaatctg gcttcgattc gcctctgcca
     8521 ggcggcgatc cgcgccgcgc atcgcggcga gcggatcgtt ctgaggcccg attaaccgcc
     8581 cctaagtcac tgggtcaatt tttcggacgg caggtgagcg cggccgctgg ccgcgccgct
     8641 tccgccagcg aaacgggggc gcctcgcgcc cgccgcggtc aacgaccgcg gctaccaggg
     8701 ctatggcgag acggcgccgt aatcggccag ttccggccgc atcgcttcga tgacgaagtc
     8761 gacgaaagcc tccggcgcgt cgagatagaa ctcgcggtcc gaatagctgc cgacctccgc
     8821 cttcaggcca tgagtccgag cccaggccgc aagttcttca aggtcggcgc gcggcgtctc
     8881 gctgaggaac gagacttgcg agcggatttt atcctcgtcg cgaccgaggc tcgcgctgcg
     8941 gctgaactgc aagtcgatat tggcgccctt ctgccgcagc cagatcgccc gctcggttct
     9001 gcgcagctca aaaaatccga gcttcgcctt cagcatttcg acgatcgtct cgaaatggcg
     9061 gtgatcgagg cggttggcga catggttgaa cgtcatcatg gcttgctcct ttcaagcgcc
     9121 gcagcgccgc cgctcaaatc accgagcaaa ttcctaagcg cttccggcgg agtcggcgaa
     9181 gccgcgcgcg cggcggacgc aaacatggtc agaggtcagt cgtctgacat ccttgactca
     9241 aggccgggca agtgcaagct ttccggcgcg acaatcggga gcgccccgct ttggaccgtc
     9301 atctgatcgc cgatctcagc gagaagatcc gagccgcggg ctcgccgcgc gacgtcctcg
     9361 tcaagcgcgt cgccgacgcc ctgcgcggtg agattgcttc aggccagttg aagcccggcg
     9421 cgcggctgat cagcgaggtc gccctcgctc aaaatcttca gatcagccgc ccgaccttgc
     9481 gcgaggccat gcgaatcctg gcgcgcgaag ggctcctcgt catcaagcac ggcgtcggcg
     9541 ccttcgtcgc cgacgagcac cggctgatct ggggccggct cgacgccatg cgctcgttca
     9601 ccgacctcat ccgctcggtc ggcggcgaac ccggcgacag ccacttgtcg atcgagcgcg
     9661 cgacggccgc ggacgaggtg gccgaggcgc tgaatattcc gccgcagacg ccggtgggcc
     9721 tcgtcaagcg cgtccggttg atcgacggcg cgccgctcgc gatcgccaat gaatacgtgc
     9781 ttctgccgaa gccggaggac gatttcgccc ggctgaagac attcgacggg gggtcgctct
     9841 acgcgttctt gcacgaacgc tgcggcgtca ccctggcgcg ctccagcgtc gtcataacgg
     9901 cgattcctgc cgacgctgcg cgcgccaagc tgctcaaagt gccgcggcga acgccgctcc
     9961 tgctcctccg cgaaccgcac tatgacgtaa gcggacggcc ggttctgttc gcggtcaact
    10021 atcacaattc gaatctcgtc cagttcaccc tcactcgggc cgggctgcgt acatgagcgg
    10081 cggctgcttc ttcgcgctgg gcaatatcag catcgacgat ctcgtctttg ccgacggcgc
    10141 gacgatgtgg gcggttcccg gcggcaacgc catctattcg gcgctcggca tggcggtgtg
    10201 gggcgagcgg gccaacgtcg ttgcgccgat cggtccggaa tatcctgtcg aaaagctcgg
    10261 cgatcgcatc gatctcagcc gatgccgacc gatcgcgcgc acccttcgcg attggggcct
    10321 ttacgaagag gacggaacgc gggaattcgt ctttcgctcg aacacccgga attggatcga
    10381 tttctgccca agcgtctcag atttgggccc gggcccctac ccgttctgcc atctcgcgcc
    10441 cctgccctgg cggcttcata tcgagctcgc ggcgacgctg cgcgaacgcg gcgccgaact
    10501 aatctcggcc gaccctgacg atcgccgcct ccacgaaatt caacgcgccg aaatcgcgca
    10561 gcttttgcgg ctggtcgaca ttttcctgcc gagccggcag gacgccgcgg caatgttccc
    10621 tgggctcagc ccgctcgacg ccttgaaagc gctgcgcgcg ctctcgcccg aaacgccggc
    10681 cattgtcgtc aaatgcggcg ccgccggcgc gatcgcccat cagcgcgacg atcgggacta
    10741 tctcgaaatc ccttcggccg cggcgcgcgc cgtcgatgaa accggcgcgg gcgacgcttt
    10801 ttgcggcggc gcgctggccg gctacgcccg gcggcgggag cttgccgccg cgctggccca
    10861 cgccgcggtt tcggcgtcct tcgcggtcga ggcgacagga tcggcggcgc ttgttgcgag
    10921 cgcttcgccg accgccgagg agcgtctcgc ccgcatctcg cggaggatcg agacgcggcg
    10981 gctttaactc tcgaacgcaa ccagatcgag acgaatcaaa ggaggcgcag ttggacgaag
    11041 aaaaaagggt catgctgcag gaaatagcga tgcagccgga cttcgtccgc gacaatctcg
    11101 acccgatgct ggacgcgacg cgcgagtccc tcgcggagca taaaggacgc gagatcagcg
    11161 ccggattcat gatcggctgc ggcgacagct attgcgccgc cctggcggcg cgcggcttca
    11221 tgatggacgc gaccggaaag atggcggagc cggtcgaggc gctcgagttc tcgcgctatc
    11281 tcgtttccta cattccgcgc gacgccttcg tcttcggcgt gtcgaattcc gggacggttt
    11341 cgcgaaccat cgaagggatc aggctggccc gcgaacgcgg cgcctggacg ttcgccgtca
    11401 ccgtcagcgc gaccaaccgc ctggcggaag cggccgaaac gctgatcaag gtcaatgcga
    11461 cgccgaacat caaggagaga ccaggcggga cgcgcatcgt cacgccgggc acggtgacct
    11521 acaccgcgag catgcttggc ctcttcgtcg ccgcgatcgc gctcggcgag aagatcggcc
    11581 atctcgacaa aagccgcgcg acgacgctga tcgaccagat tcgcagagcg gccgacgcca
    11641 tggccgccgc ggacgaaatg tcccgcacca tcgccggcga tatcgccgcc accttcctgc
    11701 cgagccgcaa gacggtaatc ctgggcggcg gcccgaatta tgcgacggcc tatttcgcca
    11761 tggccaaatg gcacgaagcg ctgacccggc cctgccatgc ctcggagctc gaagaatggg
    11821 cgcacgaaga atatttccat accgacgaga acagcgacac attcgtgctc ttgccgccgg
    11881 gcggcggcca tagccgcggc ctcgaacagg cgctcgccgc caaggagatg ggctctcgcg
    11941 ttctcatcat cggcgcggcg gacgacgagg cggcgcgaaa ggcggcggac gtctattttg
    12001 caatgcccgc cgatgttccg gaatcgctca ccccgctcgt ctacaaagcc ccgttcgagc
    12061 atctctcctg ccagatcgcg cacgagcaga agataccctt cctcggcttc cacaatccca
    12121 agcggcagca ggtcaatttc cgccaaatct tcagctccgc gcaaacgacg gccacgggat
    12181 gaagcaatgc gcaagctcct ttcgctgatc caggccggcg gcaagccgat catcggcatg
    12241 gtgcagctcg gcgccttgcc cggcggatcg cgctatcgcg gcggcgcgat cggcgagatc
    12301 gtgcaggccg cgctcgacga tgcgaccctg ctcgcccggc acggcgtcga cgcgctgatg
    12361 gtgcaaaatc tcggcgacct gccggtcgac gctcgcgtca gctcggccca gctcgcctgg
    12421 atgacgcgcg tcgcgaccga gatacgttcg cgcttcgatg gtccgcttgg attgaacttt
    12481 cttgaaaacg acgccgaggc catgctcgcc gtcgcttcgg cggcgggcgc cgatttcgtc
    12541 cggatcaaag tctatgtcgg cgccatgatg acgccgttcg ggatcgaaag cgggcaagcc
    12601 ttcgccgcca ttcgggcgcg aaacgcgctc gacgccgcgg gcgtcgcagt cttcgccgac
    12661 gtgcatgacc gcaccggcac gccgatcgcc agcggcggct tcgaagaaga cctcgattac
    12721 gccacccggc tcggcggcgc cgacggcctg gtcctgaccg gcaagaccta tgcgcagact
    12781 ctcgaattcc tgcgcggcgc gcgcgagcgc gtcgggcgga cgccgatcct tgtcggcggc
    12841 ggcgtgagcg agaagaactt cgccgaggtt gcgaaactcg ccgacggggt gatcgtcagc
    12901 tcctcgctca aggacacgtc ccgggcgttt ggcgcgttcg tcccggcgaa ggtggaatcc
    12961 ttcatgaacg aagcgagacg cgttcgctcg atgtgaacgg gcgcgcaaag gaggtcgtac
    13021 caacgacgaa aagggaggat cgcatgttga agttgcttgc gaagttcagc gcgctcgccg
    13081 tcatgacggc gagcctggct ttcagcaccg cggcggaagc caagctcaaa gtcgccttcg
    13141 tcacctcgga atcgggcctc ggcgaccgct ccttcaacga catgatgaac caaggcatga
    13201 agcgcgccgt caaggagctc ggcatcgact acgtgatcat tcagccgcgc agcatttcgg
    13261 aattccaatc gacgctggcg cgcgccgcgg gacaaggatt cgacatcatc atcggctcct
    13321 ccttcgacat gatcaagccg atgaaggcgg tggcggccgc cttcccgcag cagaaattcg
    13381 ggctggtcga cgtcggcccc gatccgatcg cgcccaatgt cgtctccagc gtgaccaagg
    13441 attgggaagg ctcgttcctg gtcggcgtga tcgccgcgaa agtgaccaag accggcacga
    13501 tcggctttgt cggcggcaag gacattccga tcatccatcg cttcttcatc ggctattatt
    13561 acggagcgaa gatggccaag ccggagacca aggttctcga aagctattcc ggcacgttca
    13621 ccgatcccgc cgccggcaag gaatatacgt tggcgctcgt caaccagggc tcggatatca
    13681 acttcgcggt cgccggcgcg accagcgccg gcgtgatcga cgcggcgaag agctcgaaca
    13741 cgttcgccat cggcgtcgat tccgaccaga actacatggc gcctgggcat gtcctgacct
    13801 cgatggtcaa gcgggtcgat acccaggcct tcgacatgat caaatcggtc gcggacggta
    13861 cgttcaaggg cggcacggtg ctctattacg gcctcaagga gggcggcgtc gacgcggcca
    13921 tggaccaata taacaagggg ctcatccccg acgacgtctt gaagcaggtc gacgatttgc
    13981 gccagaaggt gatttccggc gagatcgtcg tgccgaacta tttcgacctc aagccgggcc
    14041 agaaggaaat gggcaagccg ccgatcgcga cgccgccttc gatcgccaac gcgaaatagg
    14101 atgaaggcgc ggcggccgcg cttcgcggcc gccgcgccta caacggagaa cagcgtggaa
    14161 gccggcgtcg acctcgtcgc catgacgcat atcgccaagc gcttcgaggc gctcacggcg
    14221 atccacgacg cgtccctgac gattcggccc ggcgaaattc atgcgcttgt cggcgaaaac
    14281 ggcgccggca agaccacgct gatgaatatt ctctacggcc tgctgccgcg cgacggcggc
    14341 gagatcctgc tgcgcggcgc agcggcgaat ttttcaagtc cggccgaggc catcgcggcc
    14401 gggatcggca tggtccatca gcatttcaag ctcgcgccgt ccttcacggt gaccgagaac
    14461 atcatcctcg gggccgagcc gctgcgcggc ttcaaccgga tcaatcgcgg cgacgcggag
    14521 cgccaaaccg cggagctcgg cgcgcgtttc ggccttgacc ttgatccgcg ggcggtcgtc
    14581 ggcgacctgc ccgtgggtct tcgccagcgc gtcgaaatcc tgaaggcgct ctatcgcgac
    14641 gcggaaatcc tcattctcga cgagccgacc gcggtgctga cgccgcagga gacgcgggaa
    14701 ttgttcgcca cgatgcgcag gctggcggag agcggacggg cgatcatctt catcacccat
    14761 aaattgcgcg aggttctcgc agtttccgac agagtgtcgg ttatgcgcct cggccggatc
    14821 atctcgaccg agccgaacaa gaacctgacc gccgagcgtc tcgccagcct gatggtcggc
    14881 cgcacggtgc tgctgcgcgt cagcaaggcg aaagcgagtc cgtcggagga gaagcttctt
    14941 caagtcgagg gcctcaccgc gatcggcgat cgcggcgaga cggccgtcga caatctttcg
    15001 ctcgccgtcc gcgccggcga gatcgtcgga cttgccggcg ttcaggggaa cggccaggac
    15061 gaactcgtcc aatgcattgc cggcctgcgg cggcctttat ccggcgcggt ggcgatttgc
    15121 ggccgcgcgg ccgaccgatg ggagccgagc ggttgccggg acgccggcct tgcctatatc
    15181 cccgccgacc gcggccgcgt cggcctatcg ttggcaagcg aggtctggga aaatctgtcg
    15241 ctcggccatt tgccgtcttt ctcccgcggc ccgattctgc tgacggaaaa ggcgcggcgt
    15301 caggcgggcg cgttgatccg gaagttcgat gtgcgcggcg ccagcgaggc gaccctcgcc
    15361 aaatcccttt ccggcggcaa tcagcagaag ctccagctcg cccgcgagct gacgcgcggc
    15421 gctcgccttg tcgtcgccga acagccgtcg caaggcgtcg atatcggggc gatcgaggcg
    15481 atccaccgca ttctcgtcga tatgcgcgat caagggcgag ccgtattcgt gatctccgcc
    15541 gatatcgacg aaatcttctc gctttcagac cgcatcctgg tgatctatcg cggccgcatc
    15601 gtcgccagtc tcgcggcgga cgaagcggat gcggagacga tcggccgctt catgggcggt
    15661 ttgcacgatc agccggacat cgaggcttcc gagcatggac gagctcatgt ccagtgaggc
    15721 ggcgcccctg cggcgaagcg cgatctcgct gcgcggcctt gtacgtccgc ttgccggtcc
    15781 cgccgtcgcc gtcctgatcg cgatcgtcgt cggcgcgctg ctcgtggcgg cggtcggcca
    15841 gaatccgctc gatgtctacg tcaccttgat caagggcgga ctggtcggct ggcccaatct
    15901 ttccgtcgcc ttgcaattga cgacgcccct gatcttcacc ggcctcgccg tggccatatc
    15961 gttccgcgcc ggcttgtgga atatcggcgc ggaaggccaa atgctgatgg gcgcgctcgc
    16021 cgcgggaatc gtcggctacg ccgtctcgct tcccgtctat ctgcatattc ctctttgcgt
    16081 gatcgccgct tttctcggcg gcgcggcctg ggcgggcatt cccgcgcttc tgcgcgtcta
    16141 tctcggggtg aacgagctgg tcgtctgcct gatgatgaat ccgatcgcgc tgctgttgac
    16201 cggctatgtc tcggcgagag ctctcaaagc gcctggaccg acaaacaagc tgcccgacat
    16261 tctcgaaacg gcaattcttc ccaatttttc cctgttctcg cagctcaatg ccggcatttt
    16321 catcgcgctc gcctgctgcg ccatcgtcgc tttcgtcaac agcgcgactt tgcggggatt
    16381 tgaatggaag atgatcgggc tcaacccgcg cttcgcctat tatggcggca tcgatgtgcg
    16441 caagagcgct ctgacgattt ttcttctcag cggcggaatc gccggcctcg ccggagccga
    16501 gcaggtgctc ggcgtctata aggcctatta cgacaatttc tcgcccggct acggctttga
    16561 cggcattgcg gtggcgatgt tggcgaagaa caacccgatc ggcgtcatcc tggcggcgtt
    16621 cctgttcggc gccttgaatg gcggcagcgc cgtcctgcaa atgatgaccg gcgtcagcaa
    16681 atatctggtg caggtcctgc aattcatcat cgtgctggtg ctcgccgcgc aattctcatt
    16741 ccgctggcgg cgcgccacga ctgggcttcg ttcgtctgcc gcgcccaaag gcgggagcca
    16801 ggggctgggt aagccgccgt taagtgaatc cgaatgacaa gcatttcgcc gcaccctcat
    16861 ccggcgcttc gcgccacctt ctcccgcaag cgggagaagg attctctcct gcattgcgag
    16921 acgttcgccg aaaatgacct cgcgcgctgc gcgccccctc tcccgcttgc gggagagggc
    16981 cggggtgagg gtaagccaca gcgtgagccg ttcggcaggc tcaattcgcc caggagccgt
    17041 ccatgatctc ggcgatcgct ttttcggcca tccgcatatc cacccctttg atctatgccg
    17101 ctttgggcgg gctcctcacc tatcaggcgg gcattctcaa catcgcgctg gacgggttca
    17161 tgatcgtcgc ggcgttttcg gcgatcgcgg tcgcctatgc gaccgagagc ctgaccctcg
    17221 ggatttgcgc gggcgtcgcc agctccgtcg tgctcgccgc cctgctcgcg ctgttcaacc
    17281 tccgcttcaa ggccaatatc ttcattgccg gaatcgcggt gaccttcatg gcctatggcc
    17341 tgaccgcgct gctcctcaag ggctcgctgg gtcaggaagg cgtgttctcg tcggaccgca
    17401 ttccgacctt tccgccgctc catattccgc tcatcgccga cataccgatt ctcggtccgc
    17461 tcgtcagcgg ccatacgctt ctggtctatt tcgcctacct tctcgtgccg gtggtcgcgt
    17521 tctttcttta ccgcacgcgc tggggccttc gcgtgcgggt cgtcggcgag gccgaggaag
    17581 cggcggcggc cgcggggatc aatgtcgcga gcttgaaatt tcaaaccatg ctgtggtcgg
    17641 gcgtgttttg cggccttgcc ggctcctatc tttctctcgg ctacgtctcg ctgttcgcca
    17701 agcagatgac caacgagcgc ggcctgatcg cgctcgccgc gatcttcttc gccaatggcg
    17761 atccctattg gacggcggcc gtggcgctgc tcttcggcgc ggcgaccgcc ctttcggtac
    17821 aactgcccga gatgaccggc gccgcgccgc aactcctcca gctcatccct tatgtcgtga
    17881 cggtgctcgc cctggtcatt gtcggactgc gcaattcccg gcgccgcaac cgctacggcg
    17941 tctggcgatt cgaactgtag cgccgccctt aggcgacggc gcccgaggcg aagaagccgt
    18001 cgatcgccgc ctgatcgaag ccgaactcgc gcagcaactc ggccgaatgt tctccgatcc
    18061 gcggcgcggg gcgctcgatc gccggcggcg tttcgctcat tttcactgcc ggcgcgacga
    18121 ctttcacccg cccggccgcg gggtgatcgt aagcgccgat cgcccgcata tgggcgactt
    18181 gcggatcgtc ggcggcctcg acatgggtct tgacctcgcc gcaccagata tcggccttca
    18241 acatcgcttc gatgagatcg gcggtcgccc agctcttggt caaggcggcg agcttctccc
    18301 aaatctcgtc gcgctcctcg aaaagcgact ccggatcgtc ataggcgaga agctccgtcg
    18361 cgccgagcac gttcaccagc gtccgatagg gactcatcgc gatcgtgacc cagccgtcct
    18421 ttgtcggata gacgccgaac ggcgcctcca tgccgggatg gccaatgccg gatttcggcc
    18481 gctcgaaatc cttgccgaaa ttcatcgcca tcagcatttc ctggccctga tgggcgagca
    18541 tggcggtgag gagatcgatc ttgatctcct gtcccttgcc cgaccgctcg cgccagtaga
    18601 gcgcggcgag aatggcgtag accatgttca tcgccccgac ctgatcggag aagccggagc
    18661 cgaccggcac cggcggtccg tcgccccgtc ccgtcgccgc ggcaaggccg gtcaggccct
    18721 ggatcagcat gtcctggccg gggcggctga cataggggcc gctctcgcca tagcccgagc
    18781 ctgagcaata gatgatgcgc ggattgatcc ggcgaaaatc ctcgtagccg aagcccagcc
    18841 gcgccaaaat gccgggacga aaattctgaa ccaccacgtc cgccttcgcg ccgatccggt
    18901 agagaattgt cttcgcctcg gggctcttca gatcgacggc gatcgagcgc ttgttgcggt
    18961 tccaggcgag gaaattcggg ctttcatggc cgcccatcca gcgattgttg aacgtcatcg
    19021 tccggaagag gtcgccctgc ccgggccgct cgaccttgac gacattggcg ccgagatcgg
    19081 cgagaagctg ggtgcagaac ggcccctgca gcaaatggct gaaatcgagg accgtaatgt
    19141 ccgacaaggc cttttccgtc attgagatcg ctttctttcg tactcacgtg agataaccgc
    19201 ttagacgctc cacaacgggc gcgccgccgt gtcgctctcg attcgcgtga tatgccagcg
    19261 cccgagattg gccgtataga gcgcgcgccc atcgaacgcg atattggtcg ggtgggcgaa
    19321 aaggtgagcg gtcggatcct cgatatagac ctcggccttc ccatcttttc cgatgcgcaa
    19381 gattcgcgaa ggttcgtagc agccgcagaa gagatcgccg cgttcgtcga aggcgattcc
    19441 gtcgggcagg ccgggcaact ctatcgcaaa aggcgcggcc tcgccggcgc ttccgtctgg
    19501 cgcgacagcg atccgcgtga tctgccgcgc gaacgtctcg cagacgaaaa gcgccccgcc
    19561 cgacgcgtcg agcgccatgc cgttggcgaa gaccagcgat ttttcgtacc agagctttcc
    19621 ttcccccgtc gccaagtcga aggcccagac gccggggccg ggcgtcgcga agtcgtggct
    19681 gtcggaaaca agcagccggc tccgcgctgc gtcgacgacc ggataattcg gaatgcggat
    19741 gccgggacgc gtgaaccgct cgacccggcg cgaggcgagg tcgaggcggt agacggcctt
    19801 gtcgcgcagg tcgcaaaaga acagcgcctg atcgcgcagg aaggcgaggc cgagggtaaa
    19861 gccgccgcct tgcgcgaccc gctcgatgcg cgtcccctcc ggatcgatgc gcagcacctc
    19921 gccgttctcg ctgccgcacc aaatccagcc gtcggggccg atggcgacgc cttccggatg
    19981 ctgaagccgc ggctcgccga acaggccgtc gaaaaacacc cgcgcccgct ccatagggag
    20041 cacatggttt tcggccgtcg cgaagcgatg gttcatggcg ggaggctcct tgcaggacga
    20101 atggccgctc tcgacatcat tcccccctca cgaccagccc gccatcgacg cgcatgatct
    20161 cgccggtgat gaaggaggcg cggtcgctgg ccaggaaggc gacaagctcc gccacttcct
    20221 ccggctggcc gaaacggccg atcggatggg cgcgattcca ggtggcgatg agctcgtccg
    20281 gattgggatg ctcgcggaac actttctcgt tcaggggcgt catgaccgtt cccggcgcca
    20341 ccgcgttgac cctgatcccg aacggcgcca gctccaccgc cgcctgctgc gtcaaggcgt
    20401 tgatgccgcc cttggccgtg gcataggcgg cccagccttt gaagcccgcc agcgcctgca
    20461 ccgacgacat attgacgatg gcgcctttct tttgcttgcg catctgcggc aggcaggcct
    20521 tcatgccgcg ccacacgctg ctgagattgg tggacagaac gcgattccac gtcgcctcgt
    20581 cggtttcgtc gaccacgccg ccgatcgcct gggccgcatt gttgacgaga atgtcgatac
    20641 ggccgtactt ggcgaccgcc gcggcggcga ccgcctccag gtcggccagc gcgttgacgt
    20701 cgacgcgcag gaaatgcgcg ctgccgcctg aggcggcgat ctcggccgcg ctcgcctccc
    20761 cggccggcgc atcgatatcg gcgatgaaaa catgggcgcc gccttgcgct atcaagcgcg
    20821 cgcaggcgcg cccgatcccc atcgccgcgc ctgtgacgat cgccacccgg ccctcgaaat
    20881 cgcccatgtc atccgccttt gaaattgggt tttcgcttat ccaggaaagc cgcgacgcct
    20941 tcttcggcgt cgtccgtgcc atagagccgg aagagaactt cctgctccag cgtctgcgcc
    21001 gcgggcaagg ccgcgtcggg accgaggcgg acgagccgct tcatctcggc ttgcgccaaa
    21061 ggcgcaacag atagcatcgc gcgcgcgcgc tcgatcgccg tcgccgccag ctcctcgtcg
    21121 gggcagaccg ccgccgcaag cccgagctgg aacgcgcgct cgccggtcat ccggcgcgcc
    21181 gcgagcatca catcggaggc gacatatttg ccgacagcgc gaacgaggcg ctgcgtgccg
    21241 ccgccgcccg gcgaaaggcc gagcaggcct tccggcaggc cgagccgcgc gctcgccgcg
    21301 caaaggatga cgtcgcaaca cagcgcgatc tcgaagcctc cgcccagcgc atagccgcgc
    21361 aaagcggcga tcgtcggctt cggcagagct tccagcttgt cgaaaacgcg gcggctctcg
    21421 tgctggtagg caatgaacgc ctgggcgcgc cggccacgat attcgccgat atcggcgccg
    21481 gcgacgaaag cgcgctcgcc cgcgccggtc aggatgacga cgcgcgcgct ggcgtcagcg
    21541 gcgatgcggt cgagctcttc gtcgagacgg cggatcacct cgctcgaaag cgcgttgagc
    21601 ttttccggcc ggttgaaggt cagtcgcaca attcccggct cgatcgtctc gctcagcagc
//
LOCUS       DATGJW010000325         7561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_375139, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000325 DATGJW010000000
VERSION     DATGJW010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7561
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375139"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(339..2123)
                     /locus_tag="VKU03_10000"
     CDS             complement(339..2123)
                     /locus_tag="VKU03_10000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I adenylate-forming enzyme family protein"
                     /protein_id="HLJ71640.1"
                     /translation="MTAPPNFPALSLAAAHAALTAPGSPFEIEERDIRGVRTRVWKNA
                     PPTLREAFAAGRQHKDKTFIVYRDERVTFEALARASLAVADALQAQGLQKGDRVAIAM
                     RNMPEWAAAFFGTVVAGGVAALLNAWWKGGELEYGLIDSGATFAFADGERLERLGGHL
                     DHCGALRRIFVCRPSGGGAPRLAQPLAEIVGGVEDWSALPDRPLPSVALAPDDDATIF
                     YTSGTTGKPKGALGTHRNSCSTIMASVFTPLRAMLRRGEAPPQPNPNAPQKAALVGIP
                     LFHTTGCQAGLIPAFVHGTKLVLMHKWDAELALQLIERERVTSTGGVPTIAWQIVEHP
                     SLERYDLSSLETVSYGGAPAASELVRRIKQKFPKSSPGCGWGMSETSATFTSHVGEDY
                     ELRPDSCGPALPVCEMKIVDAMGRVLPAGEVGELWVKGPNVVKGYWRNPEATAATFVD
                     GWLRTGDIARLDEEGFCFIVDRAKDMLIRGGENIYCVEVENALFEHPAVIDAAVVPIP
                     HRTLGEEPGAVVTLKAGAKASEEEMRAFVGERIAAFKVPVRILFSQDMLPRNAAGKIM
                     KAELKALFAGGEPLLPAKRGEGGRAAAG"
     gene            2296..2694
                     /locus_tag="VKU03_10005"
     CDS             2296..2694
                     /locus_tag="VKU03_10005"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HLJ71641.1"
                     /translation="MGAIFGAKIPFADLCGIEFLGVENGVTRLRVALDERHGNNSGLP
                     HGGLVATLLDIAMGSAARFAAGSPVMTLDMHIHFMAPGETSLTAEGRVARAGGSILFA
                     EAEARDGKGDIVAKASGIFKKRKPSDGGDG"
     gene            2719..3921
                     /gene="pimC"
                     /locus_tag="VKU03_10010"
     CDS             2719..3921
                     /gene="pimC"
                     /locus_tag="VKU03_10010"
                     /EC_number="1.3.1.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018515 - pimeloyl-CoA dehydrogenase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pimeloyl-CoA dehydrogenase large subunit"
                     /protein_id="HLJ71642.1"
                     /translation="MDLRFTDDEIAFRDEVRAFIKQSLPDSIRQKLVNERHVDKDDLV
                     VWTRILNKKGWAVSHWPVEWGGTGWSAMKQLIFRDEIQQAPAPEPLAFGTSMVGPVIY
                     TFGSDAQKKRFLPRIANLDDWWCQGFSEPGAGSDLASLKTGARRDGDHWVINGQKTWT
                     TLAQYADWIFVLARTDASVKKQEGISFILVDMKTPGITVRPIQTIDGGHEVNEVFFDD
                     VRVPLENLVGQENKGWDYAKFLLGNERNGIARIGISKARLRRIRELASLPTYGDRPMM
                     EDARFRQKLAALEVELKALEMTQYRVISNERKREKGKPDPASSVLKIKGSEIQQATTE
                     LLMDVVGPYALPYREERGGDRWNEPSAGPDWAAAVAPTYFNMRKVSIYGGSNEIQRGI
                     IAKAILGL"
     gene            3938..5089
                     /locus_tag="VKU03_10015"
     CDS             3938..5089
                     /locus_tag="VKU03_10015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase"
                     /protein_id="HLJ71643.1"
                     /translation="MDFDLSEEQQLLKDSIDRLMADRYTFEKRKAHQNAPEGWSRALW
                     AQYAELGLLGLPFAEADGGFGGGAVETMLVMEAFGRALALEPYLATVVLAGGVLRRAG
                     SAAQRGALIPKIAGGELILALAHNEPQSRYDLFDVATTARRDGDAFVLDGEKGLVLHG
                     DSADKLIVSARTSGTRRDPAGIALFLVDGDSPGLTRRGYRTQDGARAAEIALAGVRVD
                     GEAVIGDPSGGFSVLESVADEAIAALCAEAVGAMQEALTQTVAYLKTRKQFGVTIGSF
                     QALQHRASDMFVALEQARSMACYAAMMASEPDEAERRKAISAAKVQIGQSARLIGQQA
                     VQLHGGMGMTMETMIGHLFKRLAMIERQFGDVDHHLEKLAEADSFVRTV"
     gene            5288..7369
                     /locus_tag="VKU03_10020"
     CDS             5288..7369
                     /locus_tag="VKU03_10020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330224.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase NAD-binding
                     domain-containing protein"
                     /protein_id="HLJ71644.1"
                     /translation="MSAINSVADLTSHGDVAVLTLNSPPVNALSAAVRDGLKRGVETA
                     DAEAGVKAIVIICAGRTFIAGADIAEFGKPPAKPSLPDVLDTIENAGKPVVAAVHGAA
                     LGGGFEVALTAHYRIAVPSAKFGLPEIKLGLIPGAGGTQRLPRLIGVEKALDVILSGT
                     PFDAASAKEWGVIDALAEEGKLLDGALAFARRLIDDRAPLRKIRALDDKIAAARGKPE
                     IFEAIRKANSRKFRGFEAWLKAIDSVRNAVDLPFDEGVAAERRMFMELLATTQSKAQR
                     YVFFAERQAAKIPDVPDDTPTRPVQSVGVIGAGTMGGGIAMNFLNAGTPTTIVETSQE
                     ALDRGLSIIRKNYENTAKKGRLTPADVEQRMALLTGTLDFGALADKDLIIEAVFEDMA
                     VKKDVFARLDAVAKQGAILASNTSYLNIDKIAATTKRPQDVLGTHFFSPANVMRLLEI
                     VRGEKTGKDVVATVIRLAKTIGKIGVLVGVCHGFVGNRMLAQRQREANKLIIEGATPW
                     DVDRVLYDFGLPMGPFAMSDLAGLDIGWSKEKSSSSTIREILCEQGRRGQKTGAGFYD
                     YDEQRNAKPSALVERIIADFAAKKGVKRRTVSDEEILERCLYPMINEGAKILEEGKAL
                     RASDIDIVWINGYGWPVYRGGPMFYGDTVGLKKVLERMRAFQAAMGDDFKPSALLERL
                     AESDGRFIDNT"
BASE COUNT         1331 a         2438 c         2524 g         1268 t
ORIGIN      
        1 gcgcggtgta ggggatttgc gtcatcgttg catccgtact gctcggaaga gtttgtgagc
       61 agccccgagg tccgcatgtt tattttctgt gtctcggcgc cggaaactca acgaggaaat
      121 ctcgaaatcg cgttatttca cgagacttcg tcgacgacga tggactcgcc tcttactttc
      181 cctggacagc cctgcgcttt gcgcggagaa ggtggcgcta agcgccggat gaggggcggc
      241 gcggaaggcg gggattggcg ttttcaggta cgtattcgat ggaagctccg tacgacgggc
      301 gctctgaaac gctaatcccc agactgcggc gctgcccctc atccggccgc tgcgcggccg
      361 ccttctcccc gctttgcggg gagaaggggt tcgccccccg caaacagcgc cttgagctcc
      421 gccttcatga tcttgccggc ggcgttgcgc ggcagcatgt cttggctgaa caggatgcgc
      481 accgggacct taaacgccgc gatgcgctcg ccgacgaagg cgcgcatttc ttcttcgctc
      541 gctttcgcgc cggctttcag cgtcaccacc gcgcccggct cctcgccgag cgtgcgatgc
      601 gggatcggaa ccacggccgc gtcgatcacc gcgggatgct caaagagcgc gttctcgacc
      661 tcgacgcagt aaatgttctc gccgccgcgg atcagcatgt ccttggcgcg atcgacgatg
      721 aagcagaagc cttcctcgtc gagccgcgcg atgtcgccgg tcctcagcca gccgtcgaca
      781 aaggtcgcgg cggtcgcttc cggattgcgc caatagcctt tgacgacatt gggccccttg
      841 acccagagct cgccgacctc gccggccggc aggacgcggc ccatcgcgtc gacgatcttc
      901 atctcgcaga ccggcagcgc cggcccgcag gaatccggcc gcaattcgta atcctctccg
      961 acatgcgagg tgaaggtggc ggaggtttcg gacatgcccc agccgcagcc gggcgaggat
     1021 ttcggaaatt tctgcttgat ccggcgcacc agctcggatg cggccggcgc gccgccgtaa
     1081 gatacggtct ccagcgagga gagatcgtac cgctccagcg acgggtgctc gacgatctgc
     1141 caggcaatgg tgggcacgcc gccggtcgag gtgacgcgct cgcgctcgat caattgcagg
     1201 gcgagctcgg cgtcccattt gtgcatcagc accagtttcg tcccatgaac gaaagccggg
     1261 atcaagccgg cctggcaacc ggtcgtatgg aaaagcggaa tgccgaccag ggcggccttt
     1321 tgcggcgcat tggggttggg ctgcggcggc gcctcgccgc gccggagcat agcgcgaagc
     1381 ggggtaaaga cgctcgccat gatcgtcgag caggaattgc gatgcgtgcc gagcgcgcct
     1441 ttcggcttgc cggtcgtgcc cgaggtgtag aagatcgtcg catcgtcgtc gggcgccagc
     1501 gcgacgctcg gcaagggacg atcgggcagc gcgctccaat cttcgacacc gccgacaatt
     1561 tccgcgagcg gctgcgccaa tcgaggcgcg ccgccgccgc tcggccggca aacaaagatg
     1621 cggcgaagcg cgccgcaatg gtccaaatgc ccgccgagcc gttcaagacg ctcgccgtcg
     1681 gcgaacgcga aagtcgcgcc ggaatcgatc aggccatatt cgagctcgcc gcccttccac
     1741 caggcgttga gcagcgcggc gacgccgccg gctacgaccg tcccgaaaaa cgccgccgcc
     1801 cattccggca tgttgcgcat ggcgatggcg acgcggtcgc ctttctgcaa gccctgcgcc
     1861 tgcaaggcgt cggcgacggc gagcgacgcc cgcgccagcg cctcgaaggt gacgcgctcg
     1921 tcgcggtaga cgatgaaggt cttgtccttg tgctggcgcc cggcggcgaa agcctcgcgc
     1981 aatgtcggcg gcgcgttctt ccagacgcgc gtcctgaccc ccctaatgtc gcgctcctcg
     2041 atttcgaaag gcgagccagg cgcggtcagc gccgcatggg ccgcggcgag cgacagggcg
     2101 ggaaagttcg gcggcgcggt catggcctcc cttcgtcgat cttgcttgtt tatgcgcgct
     2161 atgttgactc agcgctcaag cggcgacaag cgcggccgag aacgaagcgg gctcatttgc
     2221 ctcgaccggc aaagagaggc tagcctcgcg ccgccgccgg ccttccgcgt gcggcgcaag
     2281 ggagggatcg cccgcatggg cgccattttc ggggcgaaga ttcccttcgc cgatctttgc
     2341 ggcatcgaat ttctcggcgt cgaaaacggc gtcacccgcc tgcgcgtcgc gctcgacgaa
     2401 aggcatggca acaattccgg cctgccgcat ggcggccttg tcgcgacttt gctcgatatt
     2461 gcgatgggct cggcggcgcg cttcgccgcc ggctcgccgg tgatgacgct cgacatgcac
     2521 attcatttca tggcgccggg cgaaacctcg ctcaccgccg agggcagggt ggcgcgggca
     2581 ggcggctcga tcctcttcgc cgaggccgag gcgcgcgacg gcaagggcga catcgtcgcc
     2641 aaggcgagcg gaattttcaa gaagcgcaag ccaagcgacg gcggcgacgg ctgaaagaga
     2701 tttcggaggc gagataaaat ggatttgcgc tttaccgacg acgagatcgc ctttcgcgac
     2761 gaggtccgcg ccttcatcaa gcaatcgctg ccggactcga tccggcagaa gctcgtcaac
     2821 gagcgccatg tcgacaagga cgatctcgtc gtctggacgc gcattctcaa caagaagggc
     2881 tgggcggtca gccactggcc ggtcgaatgg ggcggcacgg gctggagcgc gatgaagcag
     2941 ctcatcttcc gcgacgaaat ccagcaggcg ccggcgccgg agcccctcgc tttcggaacc
     3001 agcatggtcg gcccggtcat ctatacgttc ggctccgacg cgcagaagaa aagattcctg
     3061 ccgcgcatcg ccaatctcga cgattggtgg tgccagggct tttccgagcc gggcgcgggc
     3121 tccgacctcg ccagcctgaa gaccggcgcc aggcgcgacg gcgatcactg ggtcatcaac
     3181 ggccagaaga cctggacgac gctggcgcaa tacgccgact ggattttcgt cctcgcccgc
     3241 actgacgcct cggtgaagaa gcaggagggc atttccttca ttctcgtcga tatgaagacc
     3301 ccgggcatca cggtcaggcc gatccagacg atcgatggcg gccatgaggt caacgaggta
     3361 tttttcgacg acgtgcgggt cccgctcgaa aacctcgtcg ggcaagagaa caaaggctgg
     3421 gactatgcga aattcctgct cggcaacgag cgcaacggca ttgcccggat cggcatttcc
     3481 aaagcgcggc tgcgccgcat ccgcgagctt gcctcgctgc cgacctatgg cgaccggccg
     3541 atgatggagg atgcgcgctt ccgccagaag ctcgcggcgc tggaggtcga gctcaaggcg
     3601 ctggagatga cgcagtaccg cgtgatttcg aacgagcgca agcgcgagaa gggcaagccc
     3661 gacccggctt cctcggtgct gaagatcaag ggctcggaaa tccagcaggc gacgacggaa
     3721 ttgctgatgg acgtggtcgg cccctacgcg ctgccctatc gcgaggagcg cggcggcgat
     3781 cgctggaacg agccttccgc cggcccggat tgggcggcgg cggtcgcgcc gacctatttc
     3841 aacatgcgca aagtctcgat ctatggcggc tcgaacgaga tccagcgcgg catcatcgcc
     3901 aaggcgattt tgggcttgta aacggggcgt cagaccagtg gatttcgatc tctcggaaga
     3961 gcagcagctc ctcaaggaca gcatcgaccg cctgatggcc gaccgctaca cgttcgagaa
     4021 gcgcaaggcc catcaaaacg cccctgaagg ctggagccgc gcgctctggg cgcaatatgc
     4081 cgagctcggc ctgctcggcc tgcctttcgc cgaggccgat ggcggctttg gcggcggggc
     4141 ggtcgagacc atgctggtca tggaggcgtt cggccgcgcc ctggcgctgg agccctatct
     4201 cgccaccgtg gttctcgccg gcggcgtcct gcgccgcgcc ggcagcgccg cgcaacgcgg
     4261 ggcgctcatt ccgaaaatcg ccggcggcga gctgatcctc gctttggcgc ataacgagcc
     4321 gcaatcgcgc tacgatctct tcgatgtggc gacgacggcg cggcgcgacg gcgacgcttt
     4381 cgtgctcgac ggcgagaagg ggctcgtttt gcatggcgac agcgccgata agctgatcgt
     4441 ctcggcgcgg acaagcggga cgcggcgcga tcctgcaggg atcgctcttt tccttgtcga
     4501 cggcgattcg cccggcctga cgcggcgcgg ctatcggacc caggacggcg cccgcgcggc
     4561 cgaaatcgcg ctggccggcg tacgcgtaga cggcgaggcc gtgatcggcg atccgtccgg
     4621 cggtttctcg gttctggaaa gcgtcgccga cgaggcgatc gcggcgctct gcgccgaggc
     4681 ggtcggcgcc atgcaggagg cgctgaccca gaccgtcgcc tatctgaaaa cgcgcaagca
     4741 attcggcgtg acgatcggct cgttccaggc tttgcagcac cgcgcctcgg atatgttcgt
     4801 cgctttggag caggcgcgca gcatggcctg ctacgccgcg atgatggcga gcgagccgga
     4861 cgaagccgag cggcgcaagg cgatctcggc ggcgaaggtt cagatcggcc aatcggcgcg
     4921 gctgatcggc cagcaggcgg tccaattgca tggcggcatg ggcatgacga tggagacgat
     4981 gatcggccat ttgttcaagc gcctggcgat gatcgagcgc caattcggcg atgtcgacca
     5041 tcacctcgaa aagcttgccg aggccgacag ctttgtccga acggtttgag gcgcgatcat
     5101 cctcccccgt ggagcgaagc ggaacggggg agggggacca tgcgaagcat ggtggagggg
     5161 ggctccggtc tatcaggaca aatcccaacc gccggcggcc ccctctaccg cgctagcgcg
     5221 cggtcccccc gtcttcgaag ggggaggata aagtcgccgc ctttgtacta atcgctggga
     5281 gcacgttatg tccgccatca attccgtcgc cgacctgact tcgcatggcg acgtcgccgt
     5341 gctgacgctg aattctccgc cggtgaacgc gctttcggcg gcggttcgtg acggcctgaa
     5401 gcgcggcgtc gagacggccg atgcggaggc gggcgtcaag gcgatcgtga tcatctgcgc
     5461 cgggcgcacc ttcatcgccg gcgccgacat cgccgaattc ggcaagccgc cggcgaagcc
     5521 gtccttgccc gacgttctgg acacgatcga gaacgccggc aagccggtcg tggcggcggt
     5581 gcatggagcc gctttgggcg gcggcttcga agtcgcgctc accgcccatt accggatcgc
     5641 cgtcccttcg gccaaattcg gcctgccgga aatcaagctc ggcctgatcc ccggggcggg
     5701 ggggacgcag cgcctgccgc gcctgatcgg cgtcgaaaag gcgctcgacg tcattctgtc
     5761 cgggacgccg ttcgacgcgg cctcggcgaa ggagtggggc gtgatcgacg cgctcgccga
     5821 ggagggcaag ctcctcgacg gcgccttggc cttcgcccgc cgcctgatcg acgacagggc
     5881 gccgttgcgc aaaatccgtg cgctcgacga caagattgcc gccgcgcgcg gcaagccgga
     5941 aatcttcgag gccatccgca aggccaattc ccgcaagttc cgcggcttcg aagcctggct
     6001 caaggcgatc gactcggtgc ggaacgccgt cgatctgccc ttcgacgagg gcgtcgcggc
     6061 cgagcggcgg atgttcatgg agcttttggc gacgacccag tccaaggcgc agcgctatgt
     6121 ctttttcgcc gagcggcagg ccgcgaaaat tcccgacgtg cccgacgaca cgccgacgcg
     6181 gccggtccaa tcggtcggcg tgatcggcgc cggaacgatg ggcggcggca tcgcgatgaa
     6241 cttcctcaat gccggaacgc cgacgacgat cgtcgagacc agccaggagg cgctcgaccg
     6301 cggtctttcc atcatccgca agaactacga gaacacggcg aagaagggcc gcctgacgcc
     6361 ggccgatgtc gaacagcgca tggcgctgct caccggaaca ctggatttcg gcgcgcttgc
     6421 cgacaaggat ttgatcatcg aagccgtgtt cgaggacatg gcggtcaaga aggacgtctt
     6481 cgccaggctc gacgccgtcg ccaagcaagg cgcgatcctc gcctccaaca catcctatct
     6541 caacatcgac aagatcgccg cgacgaccaa acggccgcag gacgtgctcg gcacgcattt
     6601 cttctcgccc gccaatgtga tgcggctctt ggagatcgtc cgcggcgaga agaccggcaa
     6661 ggacgtggtc gccaccgtga tccggctcgc caagacgatc ggcaagatcg gcgtcttggt
     6721 cggcgtctgc cacggctttg tcggcaatcg catgctggcg cagcgccagc gcgaagccaa
     6781 caagctgatc atcgaaggcg cgacgccttg ggacgtcgat cgcgtcctct atgatttcgg
     6841 cctgccgatg ggccccttcg cgatgagcga cctcgccggt ctcgatatcg gctggtcgaa
     6901 ggagaaatcg tcgagctcga ccattcgcga gattctctgc gagcagggcc gccgcggcca
     6961 gaagacgggc gccggtttct atgactatga cgagcagcgc aacgctaagc cgtcggcttt
     7021 ggtggagcgg atcatcgcgg atttcgccgc caagaagggc gtcaagcgcc ggactgtttc
     7081 ggacgaggag attttggagc gctgcctcta tccgatgatc aacgagggcg cgaaaatcct
     7141 cgaagagggc aaggccctgc gcgcctcgga catcgatatc gtctggatca acggctatgg
     7201 ctggccggtc tatcgcggcg gcccgatgtt ctatggcgac acggtcgggc tcaagaaggt
     7261 gctcgagcgg atgcgggcgt tccaggcggc gatgggcgac gatttcaagc cctccgcctt
     7321 actcgagcgt ctcgccgagt cggatggccg atttatagac aatacataag tcgaattgga
     7381 agggcggcgg cttgccccgc cgcagcgctc tccggcaagc ggagagcggg ctcccgcccg
     7441 ctccttcagc cgccgcgctg cagccgcaat cgccgactgc ggctacaatc ccttctcccg
     7501 ctttaagcga gagggcggcc gcgtagcggc cggatgaggg tcggcctcgg cgcccgccaa
     7561 g
//
LOCUS       DATGJW010000326         6276 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_375232, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000326 DATGJW010000000
VERSION     DATGJW010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6276)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6276)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6276
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_375232"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(208..402)
                     /locus_tag="VKU03_10025"
     CDS             complement(208..402)
                     /locus_tag="VKU03_10025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71645.1"
                     /translation="MKRRAAETEDAKKRLDERADEADRESFPASDPPAYTPTAAGAPR
                     REEDQGGAKRAQQGKKSRKP"
     gene            complement(486..1421)
                     /locus_tag="VKU03_10030"
     CDS             complement(486..1421)
                     /locus_tag="VKU03_10030"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HLJ71646.1"
                     /translation="MPFSKFARFDARPLALAFALGAVFLIAPAFADGKVLATIDGQPI
                     TQDDVNVALEDIGPGLPQSLEGAEREKYVLDYLIDLKLVARKAAADKLDSSPEFARKA
                     AYTHDKLLMEALLGNVAKSAATEDAERKVYNEAAKAQPPQEEIHARHILVPTEAEAKA
                     ALARIKAGEDFAKVASEISKDPGAQGGDLGWFTKDKMVPEFAEAAFKLQPGQVSDPVK
                     TQFGWHIIKVEERRMKKFPPFEQVKDQAARYVVQKAQSELIAQLREGAKIVRNEPPPA
                     PADAAKPGDAAKPADASKPADAAKPADAPAEQKKP"
     gene            complement(1638..3275)
                     /gene="groL"
                     /locus_tag="VKU03_10035"
     CDS             complement(1638..3275)
                     /gene="groL"
                     /locus_tag="VKU03_10035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015596587.1"
                     /note="60 kDa chaperone family; promotes refolding of
                     misfolded polypeptides especially under stressful
                     conditions; forms two stacked rings of heptamers to form a
                     barrel-shaped 14mer; ends can be capped by GroES;
                     misfolded proteins enter the barrel where they are
                     refolded when GroES binds; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chaperonin GroEL"
                     /protein_id="HLJ71647.1"
                     /translation="MASKEIKFATDARDKMLRGVDKLANAVRVTLGPKGRNVVLEKSF
                     GAPRITKDGVTVAKEIELEDKFENMGAQMVREVASKTSDTAGDGTTTATVLAQSIVKE
                     GAKYVAAGMNPMDLKRGIDIAVEAVVADLKSHAKKITSNDEIAQVGTISANGDQEIGR
                     FLADAMQKVGNEGVITVEEAKSLETELEVVEGMQFDRGYVSPYFITNAEKMRAELEEP
                     YILIHEKKLSGLQAMVPLLEAVIQTGKPLLIVAEDVEGEALATLVVNKLRGGLKVAAV
                     KAPGFGDRRKAMLEDIAILTGGQMIAEDLGIKLENVTLQMLGRAKKVVIEKENTTIVN
                     GAGKKADIEGRVAQIKAQIEETTSDYDREKLQERLAKLAGGVAVIRVGGATELEVKER
                     KDRVDDALHATRAAVEEGILPGGGVALLRALKTLDNVKPANEDQKAGVDIVRRAIQIP
                     ARQIVENAGEDGSFIIGKLVEKGDYAWGYNAATGEYQNLVKAGVIDPAKVVRTALQDA
                     ASVASLLITTEALVAEKPKKPAATPAAPGAGMGDMDY"
     gene            complement(3321..3635)
                     /gene="groES"
                     /locus_tag="VKU03_10040"
     CDS             complement(3321..3635)
                     /gene="groES"
                     /locus_tag="VKU03_10040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="co-chaperone GroES"
                     /protein_id="HLJ71648.1"
                     /translation="MHFRPLHDRVVVRRIDAEERTAGGVIIPDTAKEKPQEGEIVAAG
                     PGARNEKGELVPLDVKAGDRILFGKWSGTEVKLDGEDLLIMKESDILGVIEKAQAAKK
                     AA"
     gene            4039..4302
                     /locus_tag="VKU03_10045"
     CDS             4039..4302
                     /locus_tag="VKU03_10045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335153.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="usg protein"
                     /protein_id="HLJ71649.1"
                     /translation="MASRELLFQGYGLTTAEIRYRMPDHPSLLQLYIWQEYDVAPSFP
                     TLRGFLDYWRRELEGALHSVRVAHNRLIKPTEWRAVDGIITVN"
     gene            4371..5204
                     /gene="folP"
                     /locus_tag="VKU03_10050"
     CDS             4371..5204
                     /gene="folP"
                     /locus_tag="VKU03_10050"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013503364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HLJ71650.1"
                     /translation="MIAAPFDSAAFRALGRPAVMGILNVTPDSFSDGGLFLTPDRAIA
                     RAAEMAGQGAEILDIGAESTRPYGGAQPVAAEEEIARLAPVLPDIVKIGLPVSIDTMK
                     ASVAAFALERGAAMVNDVWGLQRDPDMARIVAGTGAAVVVMHNRERAEPAIDIIADIA
                     AFFARSLDIAARAGVAPSRIILDPGIGFGKTPAQSVVALARLGELRRFGLPLLVGASR
                     KRFIDKISPAPPERRLAGSIAAHLAAAEAGAAIVRVHDVAKTLQALKVAKAIREARCP
                     T"
     gene            5192..5560
                     /gene="folB"
                     /locus_tag="VKU03_10055"
     CDS             5192..5560
                     /gene="folB"
                     /locus_tag="VKU03_10055"
                     /EC_number="4.1.2.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016842351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004150 - dihydroneopterin aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0006760 - folic acid-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroneopterin aldolase"
                     /protein_id="HLJ71651.1"
                     /translation="MSDLIFVKGLVLHAHHGVMAHEGEVGQRFVVDLELKADLDEAAA
                     SDRLANTISYAAVVEEAQRAFVRRKFKLVEAAAGAVAQALLERFARVEEVRVVVHKPH
                     APIAAIFDDVGVALTRRRER"
     gene            5567..6055
                     /gene="folK"
                     /locus_tag="VKU03_10060"
     CDS             5567..6055
                     /gene="folK"
                     /locus_tag="VKU03_10060"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HLJ71652.1"
                     /translation="MAQALIGLGGNVGDARAALDEAVRRFCGGAGVKLAARSADYRTA
                     PWGVLDQPDFVNLCLRVETDLPPRALLARALAVEASLGRNRAAETRWGPRIVDIDLLA
                     YGDLALDEPDLVLPHPRLTERAFVLVPLCEIAPDWRIGGESVRVLAARLGAAGVERLP
                     PR"
BASE COUNT          906 a         2080 c         2042 g         1248 t
ORIGIN      
        1 gcgggtcgct ggcggggaag gaatcgtcga tcgcctgatc gagcttttcg tcctccgcgc
       61 ggcgtttttc gtgcaccttc ttggacgagt gcttatcgac agtcattgca agcgctccct
      121 taagagcttc gctgccggag agcaacgtcg gcgaaggcgc tcggttccgc gcggctggag
      181 cagcgcgtgc ggtcgcacga acgcttttta aggcttcctg ctcttcttgc cctgctgcgc
      241 gcgcttggcg ccgccctgat cctcctcgcg gcgcggcgcg ccggcggcgg tcggcgtgta
      301 ggcgggcgga tcgctggccg ggaaggattc ccggtcggct tcgtccgccc gctcgtcaag
      361 gcgcttcttc gcgtcttccg tttccgcggc gcggcgcttc atcgctaagc tcctccgctt
      421 tcattgcgcg cttgccctgc gcaatgacgg tctcggcctt gagccgatgt cgccgcgccg
      481 agccttcagg gcttcttctg ttcggcgggc gcgtcggccg gttttgcggc gtcggccggc
      541 ttgctcgcgt cggcaggttt cgcggcgtcg ccgggcttcg ccgcatcggc cggagcgggc
      601 ggcggctcgt tgcggacgat tttcgcgccc tcgcgcaatt gcgcgatcaa ttcgctttgc
      661 gccttctgca cgacgtagcg cgccgcctgg tccttgacct gctcgaaagg cgggaatttc
      721 ttcatccggc gctcttcgac cttgatgatg tgccagccga actgcgtctt gaccggatcg
      781 gagacctggc caggctgcag cttgaacgcg gcctcggcga attccggcac catcttgtcc
      841 ttggtgaacc agccgagatc gccgccttgc gcgccggggt ccttggagat ctcgctcgcc
      901 actttggcga agtcctcgcc ggccttgatc cgggcgaggg cggccttggc ctccgcctcg
      961 gtcggcacga ggatgtggcg ggcgtggatt tcttcctgcg gcggctgcgc cttggcggcc
     1021 tcgttataga ctttgcgctc ggcgtcctcg gtcgccgcgc tcttggcgac attgccgagc
     1081 agcgcctcca tcagcagctt gtcatgcgta taggcggcct tgcgggcgaa ttccgggctg
     1141 gaatcgagct tgtcggcggc cgccttgcgg gcgacgagct tcaagtcgat gagatagtcg
     1201 agcacgtatt tctcgcgctc ggcgccttcg aggctctgcg gcaggcctgg gccgatatcc
     1261 tccagcgcga cgttgacgtc gtcctgggtg atcggctggc cgtcgatcgt cgccagcacc
     1321 ttgccgtcgg cgaaagccgg ggcgatgagg aaaacggccc cgagcgcaaa ggcgagagcc
     1381 aagggacgcg cgtcgaatcg cgcgaatttc gagaacggca tggagactcc tttggcccgc
     1441 gccttcggcc ggcgtgaacg gcgcggccgg tataaagcgg aacgggcgaa ttgaaaatcg
     1501 cgttcctgca aggcgcgcgt tcaaaaaagg cttgttagcc ggaagctggg caggattagg
     1561 gccgcgggag cggaaaggcg ggcgagcgcc tcgcaaaaag aaaggcccgg ccgcgcggcc
     1621 gggcctgttt taggacctca atagtccatg tcgcccatgc cggcgccggg cgcggccggc
     1681 gtcgcggccg gtttcttcgg cttctcggcg acgagagcct ccgtggtgat cagcagcgaa
     1741 gcgacggagg ctgcgtcctg cagcgcggtg cgcacgacct tggcggggtc gatcacgccg
     1801 gccttgacga ggttctgata ctcgccggtc gcggcgttat agccccaggc gtagtcgcct
     1861 ttctcgacca gcttgccaat gatgaacgag ccgtcctcgc cggcattctc gacgatctgc
     1921 cgggccggta tctggatggc gcggcgcacg atgtcgacgc cggccttctg gtcctcgttg
     1981 gccggcttga cgttgtcgag cgtcttcagc gcgcgcagca gcgcgacgcc gccgcccggc
     2041 aggatgccct cttcgaccgc cgcgcgcgtc gcatgcagcg cgtcgtcgac gcggtccttc
     2101 ctttccttga cctcgagttc ggtcgcgccg ccgacgcgga tcaccgcgac gccgccggca
     2161 agcttggcga ggcgctcttg cagcttctcg cggtcatagt cggaggtcgt ctcttcgatc
     2221 tgcgccttga tctgggcgac gcggccctcg atgtccgcct tcttgccggc gccgttgacg
     2281 atcgtggtgt tctccttctc gatcaccact ttcttggcgc ggccgagcat ttgcagcgtg
     2341 acgttctcga gcttgatgcc gagatcctcg gcgatcatct ggccgccggt gaggatggcg
     2401 atgtcttcga gcatcgcctt gcggcgatcg ccgaagcccg gcgccttgac ggctgcgact
     2461 ttcaggccgc cgcgcagctt gttgacgacg agcgtggcga gcgcttcgcc ctcgacgtcc
     2521 tcggcgacga tgagaagggg cttcccggtc tggatgaccg cctcaagcag cggcaccatc
     2581 gcctgcaggc ccgacagctt cttttcatga atgaggatat agggctcttc gagctccgcc
     2641 cgcatttttt cggcgttggt gatgaaatag ggcgagacat agccgcggtc gaactgcatg
     2701 ccttcgacca cttcgagctc ggtttcgagg ctcttggcct cctccaccgt gatcacgccc
     2761 tcattgccga ctttctgcat cgcatcggcg aggaagcggc cgatctcctg gtcgccattg
     2821 gcggagatcg tgccgacctg ggcgatctca tcattggacg tgatcttctt ggcgtggctc
     2881 ttgaggtcgg cgaccacagc ctcgacggcg atgtcgatgc cccgcttcaa atccatcggg
     2941 ttcatgccgg cggcgacata tttcgcgcct tccttgacga tcgactgggc gagaaccgtc
     3001 gccgtggtgg tgccgtcgcc cgccgtatcg ctggtcttgg aggcgacttc gcgcaccatc
     3061 tgcgcgccca tattctcgaa cttgtcctcg agttcgatct ccttggcgac ggtgacgccg
     3121 tccttggtga tgcgcggcgc gccgaaggat ttttcgagca cgacgttgcg gcctttcggg
     3181 ccgagcgtga ccctcaccgc attggcgagc ttgtcgacgc cgcgcagcat cttgtcgcgc
     3241 gcgtcggtcg cgaatttgat ttccttggaa gccatttctc aaacctcctt tctggctgtg
     3301 acggctttgc gaggcgcgct tcaggccgcc ttcttcgccg cttgcgcctt ctcgatcacg
     3361 ccgagaatgt cgctttcctt catgatcagg aggtcttcgc cgtcgagctt gacctccgtg
     3421 cccgaccact tgccgaacaa aatccggtcg ccggctttca cgtcgagcgg gacgagctcg
     3481 cccttctcgt tgcgggcgcc ggggccggcg gcgacaatct cgccttcttg cggcttctcc
     3541 ttggcggtgt cgggaatgat gacgccgcca gccgtccttt cctcggcgtc gatgcggcgc
     3601 acgacgacgc gatcatgcaa tgggcggaaa tgcatgcaaa tcctccagag tgcggaacca
     3661 cgaatggcct gagcccggcc aagccagccg gccgaggcaa atccgagtta ggaagcgaaa
     3721 aatcgccgtt catgaggtcg tgagaaaaat tttgtcactc agaaacgaag agtgctgata
     3781 gcatgtaaca cattgattcg atggatcttt gagcctcctt tcgttttcct gccgctagca
     3841 gccgccatgc cgagcgggag caagcgccga tccccgcgcc tcctttgcga acccgcccgg
     3901 cttaattcgc gtttcccttt gacgcgatgc gacgagcctt gcttgaatgc gcgcaacgaa
     3961 agcagcgcgg aggcgggcga cgcgcggctg taaagcggaa gcggagccgg cgcgcttcgc
     4021 cttcggacgg agaacgacat ggcgagtcgg gagcttctct tccaaggtta tggcttgacc
     4081 acggccgaga tccgctaccg catgccggat catccttctc tgctgcagct ttacatttgg
     4141 caggaatacg acgtggcccc gtccttcccg actttgcgcg gctttctcga ctattggcgg
     4201 cgcgaactcg aaggcgcgct tcattcggtg cgcgtcgccc ataaccggct gatcaagccg
     4261 accgagtggc gggcggtgga cgggatcatc accgtcaact gaaatcctcg cccgcgcctt
     4321 cccgccgaag ggccgcgacg gcgcgcgtcc attcgtgata ggcgagcgcg atgatcgccg
     4381 cgccgttcga ttccgctgcc ttccgcgcgc ttggccgccc cgcggtgatg gggattctca
     4441 acgtcacgcc ggattctttt tccgatggcg gcctgttcct gacgccggat agggcgatcg
     4501 cccgcgccgc cgaaatggcc gggcagggcg ccgaaattct cgacatcggc gcggaatcga
     4561 cccggcccta tggcggcgcc cagccggttg ccgccgagga ggagatcgcc cggctggcgc
     4621 cggtccttcc ggacatcgtc aagatcggcc tgcccgtctc gatcgatacg atgaaagcgt
     4681 cggtcgcggc tttcgcgctc gaacgcggcg ccgcgatggt caacgacgtc tgggggctgc
     4741 agcgcgatcc ggacatggcg cgcatcgtcg ccggaaccgg cgcggcggtc gtcgtcatgc
     4801 ataaccgcga gcgcgccgag ccggcgatcg acatcatcgc ggatatcgcg gctttcttcg
     4861 cccgctccct cgacatcgcc gcccgcgccg gcgtcgcgcc gtcgcgcatc attctcgatc
     4921 cgggcatagg cttcggcaag acgccggcgc aaagcgtcgt tgcgctggcc cggcttggcg
     4981 agttgcggcg cttcggcctg ccgctcctcg ttggcgcctc gcgcaagcgc ttcatcgaca
     5041 aaatctcgcc ggcccctccg gagcgccggc tcgccggctc gatcgccgcc catctcgccg
     5101 ctgccgaagc cggcgcggcg attgtgcgcg tccatgatgt ggccaaaact cttcaggcgc
     5161 tgaaagtcgc caaagccatc cgcgaggcgc gatgtccgac ttgatcttcg tcaagggcct
     5221 ggtcctgcat gcccatcacg gcgtgatggc gcatgaaggc gaagtcggcc agcgcttcgt
     5281 cgtcgatctc gaattgaaag ccgatctcga cgaggccgcg gccagcgacc ggctcgccaa
     5341 tacgatttcc tacgccgcag tggtcgagga ggcgcagcgc gcctttgtcc ggcgaaagtt
     5401 caagctggtc gaggcggcgg ccggcgcggt cgcgcaagcg cttctcgaac gcttcgcccg
     5461 cgttgaggaa gtccgcgtcg tcgtccacaa gccgcacgcc ccgatcgcgg cgatttttga
     5521 cgatgtcggc gtggcgctga cccgccggcg cgagcgttga tccgctatgg cgcaagcctt
     5581 gatcggcctc ggcggcaatg tcggcgacgc ccgcgcggcc cttgacgagg cggtccgccg
     5641 cttctgcggc ggcgcggggg tcaagcttgc cgcgcgctcg gcggactatc gcaccgcgcc
     5701 ttggggcgtg ctcgaccagc cggatttcgt caatctctgc ctgcgcgttg aaaccgattt
     5761 gccgccgcgc gcgctgctcg cccgcgccct cgcggtcgag gcgagccttg gccgcaaccg
     5821 cgccgccgag acgcgctggg gaccgcgcat cgtcgatatc gaccttctcg cttacggcga
     5881 tctcgcgctc gacgagcccg atctcgtcct gccgcatccc cgcctgacgg agcgggcctt
     5941 tgtcctcgtc ccgctctgcg agatcgcgcc ggattggcgg atcggcggcg agagcgtgcg
     6001 cgtcctcgcc gcgcggctcg gcgcggcggg cgttgagcgc ttgccgccgc ggtgaagagc
     6061 gtatcccttc tccccgcgaa gcgcagggct gtccggggaa agtaagaggc gagtcgcatc
     6121 gtcgtcgacg aagtctcgtg aaataacacg atttcgagat ttcctcgttg agtttcccgc
     6181 cgagactcag aaaataaaca tgcggacctc ggggctgctc acaaactctt gtgggcagta
     6241 cggatgcaac gatgacgcaa atcccctaca ccgcgc
//
LOCUS       DATGJW010000327         3587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_789236, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000327 DATGJW010000000
VERSION     DATGJW010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3587
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_789236"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1032)
                     /locus_tag="VKU03_10065"
     CDS             complement(<1..1032)
                     /locus_tag="VKU03_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HLJ71653.1"
                     /translation="MPAQSQNSSDAKIPVTVLTGYLGAGKTTLLNRILSENHGRKYAV
                     IVNEFGEIGIDNELVVGADEEVFEMNNGCICCTVRGDLIRIIEGLMKRKGRFDAIIVE
                     TTGLADPAPVAQTFFVDQDVSAKAKLDAVVTVADAKWLADRLRDAPEAKNQIAFADVI
                     VLNKTDLVTPAELREVEARIRAINPYAKLHHAQRCNVAIDAVLERNAFDLERIMDIEP
                     DFLETGDDHDHDHDHHHDHDGHGHDHGHGHGGLKHYHDEHMQSISVRLAGDVDPDKFL
                     PWINALTQREGPNILRCKGIVAFSGDPKRFVFQGVHMMLDGEPQREWKAGEKRESKVV
                     FIGRDLKEDQ"
     gene            complement(1166..2053)
                     /locus_tag="VKU03_10070"
     CDS             complement(1166..2053)
                     /locus_tag="VKU03_10070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLJ71654.1"
                     /translation="MSSTALAAQKAPPLADRAPPHLFFLVSAIFHYLGPSFAVLLFAA
                     IAPLGVAWLRIASAAVIFALWRRPWRAFFGLSSRDRAIVVALGLVLAAMNAIFYEAIA
                     RLPLGTVGAIEFLGPIAIAAYGSRSPRNLAALGLATAGVYALADLRIAGEPLGFLFAF
                     ANCALFAFYIVLSHRIASSAGGLSSVDRLGAAMGVAAAAALPIGFRDAMPAFTSLPLL
                     LAAVGVGVSSSVIPYICDQLAMRRLPRATFALLLSLLPATASIIGFLVLRQTPSLPEM
                     AGIALVVGGVALRKDDKAE"
     gene            2128..2646
                     /locus_tag="VKU03_10075"
     CDS             2128..2646
                     /locus_tag="VKU03_10075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013531005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLJ71655.1"
                     /translation="MKREKLSPVSKSELLRDARNVEILRLLSDDPRLSVSELARRVGL
                     SAPAARERLARLEEAKVIAGYRLLVDPTALGYPVAAFIRVRPSPGQLVKIAELAQAMP
                     EVVECHRVTGEDCFILKAHLPALEDLDAVLDRFLVFGQTTTSLVQSTPVPPRALPLPS
                     ANVIPAKAGIQR"
     gene            2692..3459
                     /locus_tag="VKU03_10080"
     CDS             2692..3459
                     /locus_tag="VKU03_10080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018322225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ71656.1"
                     /translation="MSLFDLAGKSAIVTGSSRGIGRAIAIRLAEHGANVVISSRNAEP
                     CEQVAAEIAARYDEGRAVAIPANISSKDQLRRLAEEATARFGAVDILVCNAASNPYYG
                     PLASISDEQFRKILDNNVIANHWLISFVAPQMIARKDGSIILVSSIGGLRGSPAIGAY
                     NISKAADFQLARNLAAEFGPHNVRVNCIAPGLVRTDFARALWENPATLKASTARTPLG
                     RIGEPDEIAGAAVFLASKASSFMTGQTIVVDGGVTAT"
BASE COUNT          645 a         1152 c         1205 g          585 t
ORIGIN      
        1 ctgatcctct ttcagatcgc ggccgatgaa gacgactttc gactcgcgct tttcgcccgc
       61 cttccattcg cgctgcggct cgccgtccag catcatatgc acgccctgga agacgaagcg
      121 cttgggatcg ccggaaaagg cgacgatgcc cttgcagcgc aggatgttcg gaccctccct
      181 ctgggtgagc gcattgatcc agggcaggaa cttgtcggga tcgacatctc cggcgaggcg
      241 caccgaaatc gattgcatgt gctcgtcgtg atagtgcttc agcccgccat gaccatgacc
      301 atgatcatgg ccatggccat cgtggtcgtg atgatgatcg tggtcgtggt cgtgatcgtc
      361 gccggtctcc aggaaatccg gctcgatatc catgatccgc tcgagatcga aggcgttgcg
      421 ttcgagcacc gcgtcgatcg ccacgttgca gcgctgggcg tgatgcagct tggcgtaagg
      481 attgatggcg cggatgcgcg cctcgacctc gcgcaattcg gcgggcgtga cgagatcggt
      541 cttgttgagc acgatcacgt cggcgaaggc gatctggttc ttcgcctcgg gcgcgtcgcg
      601 caggcggtcg gccagccatt tcgcgtcggc gacggtgacg acggcgtcga gcttggcctt
      661 ggcggagacg tcctgatcga caaagaaggt ctgggccacc ggcgccggat cggcgaggcc
      721 ggtcgtctcg acgatgatcg cgtcgaacct gcccttgcgc ttcatcaggc cttcgatgat
      781 gcggatcagg tcgccgcgca cggtgcaaca gatgcagccg ttattcatct cgaacacttc
      841 ctcgtcggcg ccgacgacga gctcgttgtc gatgccgatc tcgccgaatt cgttgacgat
      901 caccgcatat ttgcgaccgt gattctcgct caggatgcgg ttgagcaaag tcgtcttgcc
      961 ggcgccgaga tagccggtaa ggacggtgac ggggattttc gcgtcggacg aattctggct
     1021 ttgagcaggc ataggcgaga gggtctcctg gcgcttcaag gcgggcggaa agcccggtgt
     1081 catatagggc gaagcagcgc cgatgataag ggggttgccg agcccggcag ggcgaggcgc
     1141 gcccgccggc gcgagcgggg gcctgttatt ccgccttgtc gtccttgcgc agcgcgacgc
     1201 cgccgacgac caaggcgatg ccggccattt cgggcagcga gggcgtctgc cgcagcacca
     1261 gaaacccgat gatcgaggcg gtcgccggca agagcgagag aagcagagcg aaggtggcgc
     1321 gcggcagccg gcgcatggcg agttggtcgc aaatataggg aatgaccgac gacgacacgc
     1381 cgacgccgac cgccgccaag agcagcggta ggctcgtgaa agccggcatg gcgtcgcgaa
     1441 acccaatcgg cagcgccgcg gcagccgcga cgcccatcgc cgcgccgagc cggtccacgc
     1501 tcgacagacc gccggcgctc gaagcgatgc gatggctgag cacgatatag aacgcgaaca
     1561 gcgcgcaatt ggcgaaggcg aagagaaagc cgagcggctc gccggcgatc cgcagatcgg
     1621 cgagcgcata gacgccggcg gtcgccaaac cgagcgccgc gagattgcgc ggcgagcgcg
     1681 agccataggc ggcgatggcg atcgggccga ggaattcgat cgcgccgacc gtgccgagcg
     1741 gcagcctggc gatggcttcg tagaagatcg cattcatcgc ggccagcacg aggccgagcg
     1801 cgacgacgat cgcgcggtct cgcgaggaca gaccgaagaa ggcgcgccaa ggccggcgcc
     1861 acagcgcgaa gatcacagcc gcgctggcga tccgcagcca ggcgaccccg agcggcgcga
     1921 tcgcggcgaa aagcagcacc gcgaaagaag ggccgagata gtgaaagatc gcgctgacca
     1981 ggaaaaagag gtgcggcggc gcgcggtcgg cgagcggggg cgctttctgc gcggcgaggg
     2041 cggtcgagct catggcggcg aggatgaagc ctctatcgcc gaactggaat attgacttga
     2101 aggcgaagag ccggaatcat tttcaatatg aaaagagaaa agctctctcc ggtttcgaaa
     2161 tcggaattgc tgcgcgacgc ccgcaatgtc gagatcctgc ggctgctctc cgacgatccg
     2221 cgcctcagcg tctcggagct cgcccgccgc gtcggcctgt cggcgccggc ggcgcgcgag
     2281 cgcctggcgc ggctcgaaga ggccaaggtg atcgccggct atcggttgct ggtcgacccg
     2341 accgcgctgg gctatccggt cgccgccttc atccgcgtgc ggccctcgcc cggtcagctc
     2401 gtcaagatcg ccgaactcgc ccaggcgatg cccgaagtgg tcgaatgcca cagagtcacc
     2461 ggcgaggatt gcttcatcct aaaggcgcac cttccggcgc tcgaagacct cgacgccgtg
     2521 ctcgaccgct tcctcgtctt cggccagacg acaacgtcgc tggtgcaatc gacgccggtg
     2581 ccgccgcgcg cgctgccgct gccgagcgcc aatgtcattc ctgcgaaagc gggaatccag
     2641 cgctgagatc gctattcagg cgcggcggga gttttccaaa ggaggcaatc catgtcgcta
     2701 ttcgacctcg ccggcaaaag cgcgatcgtc accggctcgt cgcgcggcat cggccgggcg
     2761 atcgcgatcc gcctcgccga gcatggcgcg aatgtcgtca tctcctcgcg caacgccgag
     2821 ccttgcgagc aagtcgcggc ggagatcgcg gcgcgatatg acgagggccg cgccgtcgcg
     2881 atcccggcca acatctcgtc gaaggatcag ctccgccgtc tcgccgagga ggcgaccgcc
     2941 cggttcggcg cggtcgacat tctcgtctgc aacgcggcgt ccaatccgta ctatggtccg
     3001 ctcgccagca tttccgacga gcagttccgc aagattctcg acaacaacgt gatcgccaat
     3061 cattggctga tctcgttcgt cgcgccgcaa atgatcgcgc gcaaggacgg ctcgatcatt
     3121 ctcgtctcct cgatcggcgg cttgcgcggc tcgcccgcga tcggcgccta caacatttcg
     3181 aaagcggcgg atttccagct cgcgcgcaat ctcgccgcgg aattcgggcc gcataatgtg
     3241 cgcgtcaact gcatcgcgcc gggcctggtc cgtaccgatt tcgcccgcgc cctctgggag
     3301 aatccggcga cgctcaaggc cagcacggcg cgaacgccgc tcggccgcat cggcgagccc
     3361 gacgagatcg ccggcgccgc ggtgttcctc gcctccaagg cgagcagctt catgaccggc
     3421 cagacgatcg tggtggacgg gggcgtgacg gcgacgtgag ccgcgcggat cccttctccc
     3481 cgctgcacgc agggctgtcc ggggaaagtc tgaggttatg gcgcgtcttg gacgtaatct
     3541 ttcagcgccg cggcgccacg ccggccgatc gccgtcaacc cgccata
//
LOCUS       DATGJW010000328        16866 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_580298, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000328 DATGJW010000000
VERSION     DATGJW010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 16866)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16866)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16866
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580298"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..412)
                     /locus_tag="VKU03_10085"
     CDS             complement(<1..412)
                     /locus_tag="VKU03_10085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLJ71657.1"
                     /translation="MPDLSAFPITKRWPAQHPDRLQLYSLPTPNGVKVSIMLEETGLP
                     YEPHKIDIGRNESWTPEFLSLNPNGKIPAILDPDGPDGKPLGLFESGAILLYLAEKTG
                     QLLPKDAGRRYETIEWVFFQMAFIGPMFGQLGYFF"
     gene            686..1108
                     /locus_tag="VKU03_10090"
     CDS             686..1108
                     /locus_tag="VKU03_10090"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLJ71658.1"
                     /translation="MTDDAKRYSGGCLCGALRYEAAGEPMAQGHCYCADCRKASGSGF
                     VPFMNFRSVDVRFRGEARQFRSRSWSGGEAVRNCCPICMSLVFGGEIGQSDSFTIYAG
                     SLDDPSRFRPTVAIFAEGRPAWAVIPPGLTVFDRMPAV"
     gene            1277..2404
                     /gene="nirK"
                     /locus_tag="VKU03_10095"
     CDS             1277..2404
                     /gene="nirK"
                     /locus_tag="VKU03_10095"
                     /EC_number="1.7.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050421 - nitrite reductase (NO-forming)
                     activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper-containing nitrite reductase"
                     /protein_id="HLJ71659.1"
                     /translation="MLTRRNLMAAAAAAALIAAAPAFADDLAAMPREKVELVAPPFIH
                     PHEQATQRPPSIVEFKMTIQEKDMVIDEKGTKIHAMTFNGSIPGPMMVVHQGDYVELT
                     LVNPETNTMAHNIDFHAATGALGGAELSLVNPGEQVTIRWKATRAGVFVYHCAPGGPM
                     IPWHVVSGMNGAVMVLPRDGLKDENGKPLHYDRAYYIGEQDFYVPRDDKGDYKTYDSL
                     ADSYPDTEEVMRKLIPTHVVFNGKEGALTGKNALSAKVGETVLFVHSEANRDSRPHII
                     GGHGDYVWETGKFANPPQKDLETWFIRGGSAGAALYTFREPGLYAYVSHNLIEAVELG
                     AAAHIKVQGQWNDDLMKQVRPPGPIGGATAGLLDGARPRPN"
     gene            2438..3313
                     /locus_tag="VKU03_10100"
     CDS             2438..3313
                     /locus_tag="VKU03_10100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614189.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SUMF1/EgtB/PvdO family nonheme iron enzyme"
                     /protein_id="HLJ71660.1"
                     /translation="MSFAMLAKGSAAIAAALTPLVWFAGAGHDRSPTRDPIAVVRIDP
                     GAFAYRQSGEFDRDGQPVNAPQRATRFDRPFFMMTTQVSAALYRRCVAAGACAPLGRS
                     GSARADLPAVGVSWRDATAFAQWLSRETGQSWRLPTDAEWAFAAGARFTDDAVVETDG
                     SFAQRWLTKYDQETALAQSLPAAPQPFGAFGANERGLEDLSGNVWEWTDTCFERQALD
                     ASSRPTAPPVVNCGVKVVEGAHRAYVSDFVRDARAGGCSVGAPPSHLGFRLVRDEPGL
                     IAATLRLLRGAGRRG"
     gene            3331..3588
                     /locus_tag="VKU03_10105"
     CDS             3331..3588
                     /locus_tag="VKU03_10105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1858 domain-containing protein"
                     /protein_id="HLJ71661.1"
                     /translation="MIDEEETVGDLMRRRPQAIRVFLDHRFGCVGCPIAFMHSVADAI
                     REHGADRAAFLGALDAMIGDSEEAPEPRRGAAHAGLKRARL"
     gene            3585..3866
                     /locus_tag="VKU03_10110"
     CDS             3585..3866
                     /locus_tag="VKU03_10110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71662.1"
                     /translation="MTEYVTAWECIGCGRIEAPRPCIGVCQDRKAEFVYARQYEEAIA
                     RAERSEREMKIAKDVLARLIKTTPQAGQWERCYRAFQSTAAKAIAACGS"
     gene            complement(4016..4348)
                     /locus_tag="VKU03_10115"
     CDS             complement(4016..4348)
                     /locus_tag="VKU03_10115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71663.1"
                     /translation="MRPAKMYVPETVQELIELLNSMLISAPKFLDKTGYLPFLNLDYV
                     FRQLREGLNHNRRALGEERYQELVRMSDQMRALFEADPEDKTGETLEGCKIIHKMEDI
                     LRQARRKS"
     gene            complement(4358..5575)
                     /locus_tag="VKU03_10120"
     CDS             complement(4358..5575)
                     /locus_tag="VKU03_10120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71664.1"
                     /translation="MGAAYGAQSEDVIFRLHRGLHRAASAIEAGDLCLAGIETILFRL
                     PDLTPLALAKLAEMAELEKVGTAWQNEPRVPAGQSDGGRWTREGSVGGAPSVNAGPAT
                     HFTPRASISSQKPALPLDGGVYRPGIDAPPRLVPTAGEEEEEEAESRLSNGPPDDFKI
                     LEDVFPGLRAALAVPLAPIAGFLGLSALADEANLEATLGQYRALVDEIKQVDPSFVDD
                     ELLPPGGIAGLSWQARANLINNLRMERAAAYYRMRGDIGPLQVETLRFLQDAVDAAYE
                     EAVSVADAGRLQPRLSREEAIGNWIDSRVRQGLRNMFTSYRIRYGPKEDVTINNRDYE
                     TTDAEEKYRMPDARLRDVSFDWTLSSKTIRTPQIRGFFRADSRPRAVIIVRPSKLGQD
                     STYLIPRPADVPF"
     gene            complement(6031..6756)
                     /locus_tag="VKU03_10125"
     CDS             complement(6031..6756)
                     /locus_tag="VKU03_10125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009030119.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HLJ71665.1"
                     /translation="MTTLDKSVLAGMPLVEGWPPGDVEALLKEARAQRYAKGAAVFEQ
                     GGEAASFFLLLHGHLQVTKITPAGQQVVVRYVAPGELFGIAVQMGRGTYPATATAVVD
                     CLALAWPSAAWPKLVARFPSLASSALQTVGSRLSDAHARVVEMSTEQVERRVAHALLR
                     IANQSGRKAEGGIEITFPITRQDLAEMTGATLYTISRILSAWEADGLIDGGRQKIFIR
                     APHRLFVLAEGREADGSPSTKEL"
     gene            6896..7348
                     /locus_tag="VKU03_10130"
     CDS             6896..7348
                     /locus_tag="VKU03_10130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudoazurin"
                     /protein_id="HLJ71666.1"
                     /translation="MLKPLFIAAIAAAAFSLAASAAEVQVKMLNKGADGAFVFEPALV
                     KIAPGDTIRFVPTDKGHNVESIAGMIPEGAAPFSGKMGEETVVVFEKPGVYGYKCKPH
                     YPMGMVGLVVVGAPGNLDVAKAVAQPGKAKTVFSALFDKLAATAASAQ"
     gene            7379..8566
                     /locus_tag="VKU03_10135"
     CDS             7379..8566
                     /locus_tag="VKU03_10135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NnrS family protein"
                     /protein_id="HLJ71667.1"
                     /translation="MAPIPRLQPTSGPALFSAGFRPFFLFGAVWAGIAIALWLPAYFG
                     DLTIATTFSPRDWHMHEMLYGYVAAIVTGFLLTAIPNWTGRLPLQGWPLVGLVATWLA
                     GRAAIAFSALIGGWTAAAIDLSFLALVIAVSARELSHSKQSHNRRVLVAVLILFAGNA
                     TFHAEALLRGAADYGVRIGLAAILLLIMLIGGRIVPSFTRNWLARENPGPLPASPGRF
                     DLIAIACGAAALLFWIVAPETSLAGAALVAAGALHAARLARWRGERTWRDRLVLVLHV
                     AYAFVPLGFFATGLAAFGLAPSSVGIHIWTIGAIGGMTLAVMTRASLGHTGRALVAGS
                     VAQAVYALIVAAALARVCAAMHPDWSMALLIASGVAWIAAFIGFAASYWPILTRPRKT
                     HQN"
     gene            complement(8686..10731)
                     /locus_tag="VKU03_10140"
     CDS             complement(8686..10731)
                     /locus_tag="VKU03_10140"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0004359 - glutaminase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HLJ71668.1"
                     /translation="MAAFSSLYSHEFIRIAACVPEARVADPDYAASATLRLAETGHRE
                     KIALMLFPELGLSSYAIDDLLFQDALLDSVERAIDRIATASQNLFPALVLGAPLRVEG
                     RLYNTAVVIHRGAILGIAPKTYLPNYREFYERRHFTSGAGIVGREATIAGRKAPFGVD
                     LLFRSSGFAPFTFHVEICEDVWAPLPPSTYAALAGAEILLNLSASNITIGKADVRRLL
                     CASHSSRAIAAYAYSAAGPGESTTDLAWDGQAAIFELGDRLAESERFPRGAVMASADI
                     DLGRLRQERMRVNSFGECARQELGKAPPFREASFRLDAVEAPVALQRRIERFPFVPAD
                     PARLRADCYEAYNIQVQGLAQRLTASGAERAVIGVSGGLDSTQALIVAARAMDLLGRD
                     RKHVLAYTLPGFATSEATKVNAWALIRALGATGEEIDIRPAARQMLGDLGHAFAKGEA
                     VYDVTFENVQAGLRTDYLFRLANQHRGLVVGTGDLSELALGWCTYGVGDHMSHYNVNA
                     SVPKTLIQHLIRFVARSGDVSEETAAVLRAVLATEISPELTPPSADRPIQSTQQVVGP
                     YALQDFNLFYLTRFGFRPSKIAFLALNAWADPDRGSWPADIPDEDRRAYSLGEIRHWL
                     EIFLRRFFANQFKRSTLPNGPKITSGGSLSPRGDWRAPADAHADIWLAELERNVPKR"
     gene            10921..12321
                     /locus_tag="VKU03_10145"
     CDS             10921..12321
                     /locus_tag="VKU03_10145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020188176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phospholipase D-like domain-containing protein"
                     /protein_id="HLJ71669.1"
                     /translation="MLSWLSYAASPIHVLLALWVTCHVLLHKRDVRAAIGWIGTAWLA
                     PIVGALLYFAFGISRVKRRARRLMGANRAIDPSLGRDVSSDDPVERLKIAISRITGEE
                     ISAGKVVAILDSGDEAYPHMLAAIDAAKTSVDLSTYIFRTDFVGTKFIAALTRARQRG
                     ATVRVLIDGFGGGFLLSPAYRALRKNGVPAARFLHSALPWKMPLLDLRLHKKTLIVDR
                     AAAYIGGLNIGAENSNEKPAKKATVRDLHFRVEGPVVHQIEEDFDEDWTFATGEPPAM
                     TPRQPDISASGDAARVIVAGPDQDEDQLALVLLSAINSAQHSIRIATPYFLPDESLVT
                     ALQLAALRGVDVQIALPAVNNHLLVAWASRPHIEPLLQTGCRIWRGPPPFDHSKLMTI
                     DGKWSLIGSSNWDVRSLRLNFEIALECHGADLARRLSELFDRKRIQPVTLQEIQNRSV
                     ILKLRDAAARLMAPYL"
     gene            12339..13061
                     /locus_tag="VKU03_10150"
     CDS             12339..13061
                     /locus_tag="VKU03_10150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HLJ71670.1"
                     /translation="MKIASYNIHKCRGVDGRVRPDRIVSVIGEMAADLVALQEVDGRF
                     GRRGGLLDPAAIRRETGMHLLVQSDAPDRHGWHGNALLVRGEPKSYRRARLKLPGFEP
                     RGAIVAEIDLGEGEFRVIAAHFGLFRRSRMDQALALLTAFNDLPPMNTILLGDLNEWR
                     RRRRSALEILEPTFGPAPTVLSFPARRPVFPLDCIFGWPGGLIGEIAAHDTPLARRAS
                     DHLPLTARAHLKVRQPILREAA"
     gene            13193..13609
                     /locus_tag="VKU03_10155"
     CDS             13193..13609
                     /locus_tag="VKU03_10155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013420293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20 family protein"
                     /protein_id="HLJ71671.1"
                     /translation="MDTRFSRYWTWSEAFELLARADRMHRQFFRPVGSPSPVAWEPPV
                     DVIETERAVLVLVALPGVDLGEAKASLEGDEMVFSGMREFPPEMQTAAIHRLELPQGR
                     FERRVRLPPGRYASVRRSESKGCLLITLEKAGAFNG"
     gene            13602..16013
                     /gene="lon"
                     /locus_tag="VKU03_10160"
     CDS             13602..16013
                     /gene="lon"
                     /locus_tag="VKU03_10160"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018647935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HLJ71672.1"
                     /translation="MADADLKDASSDNAGAGAGVSPPLPTDALIILPVRGLVLFPGMV
                     LPIALGRPRSILAAQRAVREQRPVGVLMQRNAEAEDPIPVDMHRMGTIANVVRYVTTP
                     DGAHHLVCQGEQRFRIVDYLNGWPFFVARAERISEPSALSADIEARFVHLKGQALEAL
                     QLIPQAPPELSAAIQNAESPAALSDMVATFMDATSDEKQEILETIDIVPRLDRVSRLL
                     AHRIEILRLSQEIGRETKASLDARNREFLLREQMAAIQRQLGETDEGKGAEIAELEKA
                     IAKANMPKEVEDQARKELKRLQRMPEAAGEYGMVRTYLDWLIELPWALPEEAPIDIAA
                     AHRILDEDHFGLEKIKRRIVEYLAVRKLAPGGKAPILCFVGPPGVGKTSLGQSIARAM
                     NRKFVRVSLGGVHDEAEIRGHRRTYIGALPGNIIQAIRKAGARNCVMMLDEIDKLGAG
                     IQGDPGSALLEVLDPEQNNTFRDNYLAVPFDLSRVVFICTANMLDTIPGPLRDRMEII
                     SLAGYTASEKLQIARRYLVARQLEANGLKPDQVAIADGVLETIIQHYTREAGVRNLER
                     EIGRVLRQAAMRIAEGERGSIAIASADLATILGPPPFENEVAMRTSVPGVATGLAWTP
                     VGGDILFIEATATPGNGRLILTGQLGEVMRESAQAALSIVKNRAVSFGIDPNRFEKTD
                     IHIHVPAGATPKDGPSAGVAMFMALVSLLTEKTVRSDTAMTGEISLRGLILPVGGIKE
                     KVVAAHRAGIKRIMLPARNHKDYEEIPEEVREQMEFVWLERVDDAAAQALEPAAPENT
                     ARKLA"
     gene            complement(16084..>16866)
                     /locus_tag="VKU03_10165"
     CDS             complement(16084..>16866)
                     /locus_tag="VKU03_10165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosine kinase"
                     /protein_id="HLJ71673.1"
                     /translation="GVSAAYIGKVKDDELGRAFIHDIRATGVGFSVKPAANGPATGRC
                     YIFVTPDGERSMNTYLGASQHLSPPDLDENLVREARIVYLEGYLWDRQAAKETFQKAA
                     RLAHGAGARVALTLSDSFCVDRFRAEFLDLIRSGLVDTVFANTEEVHSLYQTADFKTA
                     LEALRAEGALGVVTRSEKGSIVVDGKETFEVPAYAIDRLIDTTGAGDLFAAGFLAGMA
                     RGAGHVKSARLGALAAAEIIQHLGARPQLRLADLAAENGLAV"
BASE COUNT         2971 a         5491 c         5389 g         3015 t
ORIGIN      
        1 tgaagaaata gccgagctgg ccgaacatcg ggccgataaa cgccatctgg aagaacaccc
       61 actcgatcgt ctcgtagcgc cggccagcat ctttcggcag gagctggccg gtcttctcgg
      121 cgagataaag caggatcgcg ccggattcga acaggccgag cggcttgccg tccggtccgt
      181 cgggatccag gatcgccggg attttgccgt tcggattcaa ggacagaaat tcgggcgtcc
      241 agctctcgtt cctgccgatg tcgatcttgt gcggctcgta aggcagcccg gtctcctcca
      301 gcatgatcga caccttgacg ccgttcggcg tcggcaacga ataaagctgc agccggtcgg
      361 gatgctgcgc cggccagcgc ttggtgatcg gaaaagcgga aagatcgggc attcagggct
      421 ccggagcgac agggaatcaa acggaacata atgtcgtttc ggcgcggcgc aatggcgcaa
      481 ccgccgacga gagcggcccg cgatggttaa gcagcgaaag gaggcgatgt tcctggccgc
      541 aagcgattgc gcccgaagcc gcaggcgcgc tgaccctacg ccgcctttgg gcttatggtg
      601 cctcgtgcta tctggcgttc gcgatcgggg cggcccggat tgcccgcctc ggccgctgcg
      661 catctgagca gagcgagcca tcgccatgac cgacgacgcc aaacgatatt ccggaggctg
      721 cctttgcggc gccttgcggt acgaggccgc cggcgagccg atggcgcagg gccattgcta
      781 ttgcgcggat tgccgcaagg cgtccggctc cggcttcgta cctttcatga atttcaggag
      841 cgtggacgtg cgcttccgcg gcgaagcgcg ccaattccgg tcgcgctcgt ggagcggcgg
      901 cgaagcggtg cgcaattgct gcccgatttg catgagcctc gtctttggcg gcgagatcgg
      961 acaatcagat tctttcacca tttatgccgg gtcgctggac gatccgtcgc gcttccgccc
     1021 cacagtggcg attttcgcgg aaggccgccc cgcttgggcg gttattccgc ccggcctgac
     1081 ggttttcgat cgcatgccgg cggtttgata ttgccgaaca taggtgaacc ctgaagcatt
     1141 atgaccgcgc gctcgtcccg gccatggcag gtagggcgcc gcgagcggga cctcgattct
     1201 atttgcgctg ccgcaaagac gtttcgcccg ctcttgggca tatggcggcc tagccaagta
     1261 tggatggagc cgctcgatgt tgacccgccg gaatttgatg gccgccgcag ccgcggcggc
     1321 gctgatcgcc gccgccccgg ctttcgccga cgatcttgcg gcgatgccgc gcgagaaggt
     1381 cgaactcgtc gcgccgcctt tcatccatcc gcatgaacag gcgacgcagc gcccgccctc
     1441 gatcgtcgag ttcaagatga cgatccagga aaaggacatg gtcatcgacg aaaagggcac
     1501 gaagatccac gccatgacgt tcaacggctc cattccggga ccgatgatgg tggtgcatca
     1561 gggcgactat gtcgaattga cgctcgtcaa tcccgaaacc aacacgatgg cgcacaatat
     1621 cgatttccat gcggcgacgg gcgcgctggg cggcgcggag ctgtctctgg tcaatccggg
     1681 cgagcaggtg acgatccgct ggaaggccac ccgagccggc gtcttcgtct atcactgcgc
     1741 gccgggcggg ccgatgatcc cctggcatgt cgtctcgggc atgaacggcg ccgtgatggt
     1801 gctgccgcgc gacggcctca aggacgagaa cggcaagcct cttcattacg atcgcgccta
     1861 ctacatcggc gagcaggact tctatgtccc gcgcgacgac aagggcgatt acaagacgta
     1921 cgacagcctc gccgattcct atccggacac cgaggaggtc atgcgcaagc tgatcccgac
     1981 gcatgtcgtc ttcaacggaa aggaaggcgc gctcaccggc aagaacgctt tgagcgcgaa
     2041 ggtcggcgag acggtgctgt tcgtccatag cgaggccaat cgcgacagcc ggccgcatat
     2101 tatcggcggc catggcgatt atgtgtggga gaccggcaag ttcgccaatc cgccgcaaaa
     2161 ggatttggag acctggttca ttcgcggcgg ctcggcgggc gcggcgctct acacgtttcg
     2221 cgagcccggc ctttacgcct atgtgtcgca caatttgatc gaagcggtcg aactcggcgc
     2281 cgccgcccat atcaaggtgc agggtcagtg gaacgacgac ctgatgaagc aggtccgccc
     2341 gcccgggccg atcggcggcg cgacggcggg ccttctcgac ggggcgcggc cgcgtcccaa
     2401 ctgagccggc gcggcgcatc tttttcgggg ctcgatcatg tcattcgcga tgctcgccaa
     2461 gggctcggcg gcaattgcgg ccgcgctgac gccgctcgtc tggttcgccg gcgcgggtca
     2521 cgaccgctcg ccgacccgcg atccgatcgc ggtcgtccgc atcgatccag gcgccttcgc
     2581 ctatcgccaa tccggcgaat tcgatcgcga tgggcagccg gtcaatgcgc cgcagcgcgc
     2641 gacgaggttc gaccggccgt tcttcatgat gacgacgcag gtcagcgcgg cgctctatcg
     2701 ccgctgcgtc gccgcgggcg catgcgcgcc gctcggccgc agcgggtcgg cgcgggcgga
     2761 tttgccggcg gtcggcgtca gttggcgcga cgctacggct ttcgcgcaat ggctgtcgcg
     2821 cgagacgggc caatcctggc gcctgccgac cgacgccgaa tgggctttcg ccgccggcgc
     2881 gcgcttcacg gacgacgcag tcgtcgagac tgacggctct ttcgcgcagc gctggctgac
     2941 caaatacgat caggaaacgg cgctggcgca gtccttgccc gccgcgcccc agcctttcgg
     3001 cgcgttcggc gccaatgagc gcgggctcga agacctttcc ggcaatgtct gggagtggac
     3061 cgacacgtgt ttcgagcgcc aggcgctcga cgcctcttcg cgcccgaccg cgccgccggt
     3121 cgtcaattgc ggcgtgaagg tcgtcgaggg cgcgcatcgg gcctatgtct cggacttcgt
     3181 ccgcgacgcg cgggccggcg gctgctcggt cggcgcgccg ccgagccatc tcggctttcg
     3241 cctcgtccgc gacgagccgg gcctgatcgc ggcgacgctg cgcctgctgc gcggcgccgg
     3301 ccggcgaggc tgagacgaag gcgcggagcg atgatcgacg aagaggaaac cgtcggcgac
     3361 ctgatgcggc ggcgcccgca agcgatccgc gtcttcctcg accatcgctt tggctgcgtc
     3421 ggctgtccga tcgccttcat gcactcggtc gccgacgcga tccgcgagca cggcgcagat
     3481 cgcgccgctt tccttggcgc gctcgacgcg atgatcggcg acagcgagga agcgccggaa
     3541 ccacgccgcg gcgcggcgca cgccggcctg aagagggcga ggctatgacc gagtatgtaa
     3601 cggcgtggga atgcatcggc tgcggccgta tcgaggcgcc ccggccctgt atcggcgtct
     3661 gccaggatcg caaagccgaa ttcgtctacg cgcgacaata cgaagaagcg atcgcacgcg
     3721 ccgagagaag cgagcgggag atgaaaatcg cgaaggatgt tctggcgcga ctgatcaaaa
     3781 cgacgccgca ggcgggccaa tgggagcgct gctaccgcgc ctttcaaagt acggccgcca
     3841 aagcgatcgc cgcatgcggc tcataaagca tcgctcacgg acgctttccg tggtccaatt
     3901 cttctgaaga ctctcgctcc gccgtggctt cttccgagcc aaggcgcaac aaaaatgaga
     3961 acttgccgcg atagatcaat aaacaagaca atcgtgcgag cgatcgtggt tagcgtcagg
     4021 actttcgccg cgcctgtctc aagatgtcct ccatcttgtg gatgatcttg cagccctcca
     4081 acgtttcgcc cgtcttgtct tccgggtcag cctcgaagag cgcccgcatc tgatccgaca
     4141 ttcgcaccag ctcctgataa cgctcttctc cgagcgctcg ccgattgtga ttgagccctt
     4201 cgcgcaattg ccggaaaaca taatccaggt tcaggaacgg caaatagccc gtcttatcaa
     4261 ggaatttcgg cgcagagatc agcatagaat ttaacaattc tataagttcc tgtactgtct
     4321 ccgggacata catctttgcc ggtctcatga gtcaccctca aaatggaaca tcggcgggtc
     4381 gagggattag gtaagtacta tcttgtccaa gtttactcgg gcggacgatg atcactgcgc
     4441 gcggccgcga gtcggcgcgg aagaatccgc ggatttgcgg cgttcggatt gtcttcgaag
     4501 aaagcgtcca atcaaacgat acatctctaa gccttgcgtc aggcattcta tatttttctt
     4561 cggcgtccgt cgtctcgtag tcgcgattgt tgatcgtcac atcctctttc ggaccatagc
     4621 gaatgcggta ggatgtgaac atattcctga ggccctgccg aaccctggaa tcaatccaat
     4681 tgccgatagc ttcttctctc gacaagcgcg gctgaaggcg accggcgtcg gcgacgctga
     4741 cagcttcttc gtaagcggca tcgaccgcgt cctgcaagaa ccgcagcgtc tcgacctgaa
     4801 ggggcccgat atcgccgcgc attcgataat aggcggcggc gcgctccatg cgaagattgt
     4861 tgatcagatt ggcgcgcgcc tgccaggaaa ggccggcgat tccgcctggc ggcaggagct
     4921 catcatccac gaagctgggg tcgacttgtt tgatttcgtc aaccagcgct cgatattgac
     4981 ccaacgtcgc ttccagattg gcctcgtcgg cgagagcgct aaggccaaga aagccggcga
     5041 tcggcgccaa gggaacggcc aacgccgccc tgagaccggg gaaaacgtct tcgagaatct
     5101 tgaaatcgtc cggcggcccg ttcgataggc gagactccgc ttcttcttcc tcctcctctc
     5161 cggccgtagg gacaaggcgc ggcggcgcat cgattccagg tcgatagacg ccgccgtcca
     5221 acgggagcgc aggcttctga ctggaaatcg aggcgcgagg agtgaaatgc gttgcagggc
     5281 cggcgttgac tgagggcgcg ccgcccacgc ttccctctct cgtccatcgg ccgccgtccg
     5341 actgtccggc cggaacacgc ggctcgtttt gccaggctgt cccgactttc tccagttccg
     5401 ccatttcggc gagcttggcc aaagctaaag gagtcaggtc gggtagacgg aagagtatgg
     5461 tctcgatgcc agcgagacag agatcgcccg cctcgatcgc cgaagccgcc cggtgcagac
     5521 ctcgatgaag gcgaaaaata acgtcctcgg attgcgcccc ataggcagcg cccatgattc
     5581 gtcccaatcc ctgccatggc cgcacctcgt aacggcgtcc tcccgtcgca ttggggtcga
     5641 ggcgaaccag gtccgccggc cctaacgcga cgccagcgcg gtcgcatgcg agtccatctc
     5701 cgcccctcgg agtcagtcga tacatttgcc ggttcatcgt gagatgcttg cctctttcgc
     5761 cggacgcatt ttgttcttgt tttgttctaa agtcaagaaa tttgcgacag agacttcgca
     5821 cgatcagcgg tgcggggacg gccagcgcga taacgcggcg gcgtatgcgg cccccttctg
     5881 cctccgcacg ttcgcctggc gggctttggc tcggccagga cgcgcgagtg actccggccg
     5941 gggcgatccg tcgattcgca accaacagag attcgcgacg ctcactcatc tttcggtcag
     6001 accgggcggc ggcgaatggc gacgatcgcc tcaaagctcc ttggtggagg gcgatccgtc
     6061 cgcctccctg ccttccgcga gaacgaacag gcgatgcggc gccctgatga aaatcttttg
     6121 ccggccgccg tcgatcagcc catcggcctc ccaggcgctc agaatccggc tgatcgtgta
     6181 gagcgtcgcg ccggtcatct cggcaaggtc ctgtcgggtg atcggaaaag tgatctcgat
     6241 cccgccttcg gctttgcggc ccgattgatt ggcgatgcgc agcagcgcgt gcgcgacgcg
     6301 ccgctcgact tgctcggtcg acatctcgac cacccgcgca tgcgcgtcgc tcaaccggct
     6361 gccgacggtc tggagcgcag acgaggcgag agaaggaaag cgcgcgacca gtttcggcca
     6421 ggcggcggac ggccaggcca gagccaggca gtcaaccacc gccgtcgcag tcgccggata
     6481 agtcccccgg cccatctgca cggcgatgcc gaacagttcg cccggcgcga catagcggac
     6541 gacgacctgc tggccggccg gcgtgatctt cgtcacctgg agatggccgt gcagcaaaag
     6601 aaagaaggac gccgcctcgc cgccctgctc gaagaccgcg gcccccttcg cgtagcgctg
     6661 ggcgcgcgcc tctttgagca gcgcctcgac gtcgccgggc ggccagcctt caaccagcgg
     6721 cattcccgcc aggacggatt tatcgagcgt cgtcatttgg cggccctatg ccaaggcgct
     6781 tgcgcgccgg gcctgtgccc catcgcgctg caatttgtcc taccgcaaag accgcgtccg
     6841 gccaaacggc tacgccattc ccgtctcaaa tcggtctttc aaaaggatga cttcaatgtt
     6901 gaagccgctg ttcatcgccg cgatcgccgc ggccgccttc agcctcgccg cctcggcggc
     6961 ggaagtgcag gtcaaaatgc tcaacaaggg cgcggacggc gcgttcgtgt tcgagccggc
     7021 gctggtcaag atcgcgcccg gcgacaccat ccgcttcgtt ccgacggata agggccacaa
     7081 tgtcgaatcg atcgccggca tgatcccgga gggcgcagcg cccttctccg gcaagatggg
     7141 ggaggaaaca gtcgtcgtct tcgagaagcc cggcgtctac ggctacaaat gcaagcccca
     7201 ttacccgatg ggcatggtcg gcctggtcgt cgttggcgcg cccggcaatc tcgacgtcgc
     7261 caaggcggtc gctcagccgg gcaaggccaa gacggtgttt agcgcgctgt tcgacaagct
     7321 cgcggcgacg gcggccagcg ctcagtagcg gcgaaacttg acgaaaggag aatcgctcat
     7381 ggctccgatc ccccgcttgc agccgacctc cggcccggcg ctcttttcgg ccgggttccg
     7441 gcccttcttc ttgttcggcg ccgtctgggc gggaatcgcc atcgctttat ggctgccggc
     7501 ctatttcggc gacctgacga tcgccacgac cttttcgccg cgcgattggc acatgcacga
     7561 aatgctctat ggctatgtcg ccgcgatcgt caccggcttc ctgctcaccg cgatccccaa
     7621 ttggaccggc cgcctgccct tgcaaggctg gccgctggtc gggctggtcg cgacctggct
     7681 ggctggccgc gcggcgatcg cgttttccgc tctgatcggc ggatggactg ccgccgccat
     7741 cgacctttcc ttcttggcgc tggtgatcgc cgtttcggcg cgcgagctca gccatagcaa
     7801 gcaaagccac aaccgacggg tgcttgtcgc ggtgctgatc ctgttcgccg gcaacgccac
     7861 tttccacgcc gaggcgctgt tgcgcggcgc ggccgattac ggcgtcagga tcggcctcgc
     7921 ggcgattttg ctcctgatca tgctgatcgg cggacggatc gtcccgagct tcacgcgcaa
     7981 ctggctggcg cgcgaaaatc ccggtccgct tcccgcttcg ccgggccggt tcgacttgat
     8041 cgccatagca tgcggcgcgg ccgcgctcct tttctggatc gtcgcgccgg aaacctcgct
     8101 tgccggcgcg gcgctcgtcg cagccggggc gctacacgcc gcgcgcctgg cgcgctggcg
     8161 cggtgagcgg acctggcgcg accgcctcgt cctggttctg catgtcgcct acgccttcgt
     8221 gccgctggga ttctttgcga cgggcctcgc cgcattcggc ctggcgccga gctcggttgg
     8281 gattcacatt tggacgatcg gcgcgatcgg cggcatgacg ctcgccgtca tgacccgggc
     8341 gagcctcggc cataccggcc gcgcgctcgt cgccggctcc gtcgcgcagg cggtttacgc
     8401 gctgatcgtg gcggccgcgc tcgcacgcgt ctgcgccgcg atgcatccag actggagcat
     8461 ggcgctgctc atcgcctccg gcgtcgcgtg gatcgccgcg ttcatcggct tcgccgccag
     8521 ttattggccc attctgacgc ggccgaggaa gacgcaccag aattgaacgc gagatgcgga
     8581 ccatcattgc gaggcgcgaa gggacgaagc aatccattcg taaagcacag ccctacgaat
     8641 agattgcttt gcttcgcttc gctcgcaatg gcggcgcggt acgccttagc gcttcgggac
     8701 gttccgctcc aattcggcga gccaaatatc ggcatgcgcg tccgctgggg cgcgccaatc
     8761 gccgcgcggc gacagcgagc cgccggaggt gattttcggc ccattcggca aggtcgagcg
     8821 cttgaattga ttggcgaaga aacggcgcag gaaaatttcc agccaatgtc tgatctcgcc
     8881 cagcgagtag gcgcgccggt cctcatcggg tatgtccgcc ggccatgagc cgcgatcggg
     8941 gtcggcccag gcgttgagcg cgagaaaggc gattttggag ggccgaaagc cgaagcgggt
     9001 gaggtagaaa agattgaaat cctgcagcgc ataaggaccc acgacttgct gcgtcgactg
     9061 gatcggacgg tcggcggaag gcggcgtcaa ttccggcgaa atttccgtgg cgagaacggc
     9121 gcgcagcacc gccgctgtct cctcgctgac atcgccggac cttgcgacaa agcggatcaa
     9181 atgctggatc agcgtcttcg gcaccgaggc gttgacattg taatgcgaca tgtgatcgcc
     9241 gacgccatag gtgcaccagc cgagcgcgag ctcggaaaga tcgccggtcc cgacgaccag
     9301 gccgcgatgc tggttggcta gacgaaagag gtagtcggta cgcaagcccg cctggacatt
     9361 ttcaaacgtc acgtcgtaaa ccgcctcgcc cttggcgaaa gcatggccga gatcgccgag
     9421 catctggcgc gccgccggcc ggatgtcgat ctcctcgccg gtcgctccga gcgcgcggat
     9481 gagcgcccag gcgttgactt tcgtcgcctc ggaggttgca aagccgggca gcgtataggc
     9541 gagcacgtgt tttcgatcgc ggccgagcag gtccatcgcc cgcgccgcga cgatcagcgc
     9601 ctgggtggag tcgaggccgc ctgacacgcc gatcacggcg cgttccgcgc cgctcgccgt
     9661 caggcgctga gcaaggcctt gaacctggat gttgtacgct tcgtagcaat cggcgcgcag
     9721 cctcgccggg tccgccggca cgaagggaaa gcgctcgatc cgacgctgca gcgccaccgg
     9781 cgcctcgacc gcgtcgagac ggaacgaggc ttcgcgaaag ggcggcgctt tgccgagctc
     9841 ctggcgcgcg cactcgccga aactattgac ccgcatccgc tcttgccgca ggcggccgag
     9901 atcgatatcg gcgctcgcca tgacggcgcc tcgcggaaac cgctcgcttt cggcgaggcg
     9961 atcgccgagc tcgaaaatcg ccgcctgccc gtcccaggcg agatcggtcg tcgattcgcc
    10021 cggacccgcg gctgaatagg cataggcggc gatcgcgcgc gacgaatggc tggcgcagag
    10081 gagccgccgc acatcagcct tgccgatcgt aatgttgctc gccgagagat tgagaaggat
    10141 ttcggcgccg gccaaagcgg cgtaagtcga gggcggcagc ggcgcccaaa cgtcctcgca
    10201 tatctcgaca tgaaacgtga acggcgcgaa tcccgacgag cggaagagga gatcgacgcc
    10261 gaagggagct ttgcgtccgg cgatcgtcgc ctccctgccg acaatcccgg cgcccgaggt
    10321 gaaatgccgc cgctcgtaga actcgcgata gttcggcaga taggtcttcg gcgcgattcc
    10381 aaggatggcg ccgcgatgga taacgacggc cgtgttgtag agccgacctt cgactcgcag
    10441 cggcgcgccg agcacgagcg ccggaaacaa attttgcgaa gccgtcgcga tcctgtcgat
    10501 cgccctttcg acggaatcga gcagcgcgtc ttgaaacagg agatcgtcaa tcgcgtaaga
    10561 cgaaaggccg agctcgggaa agagcatcaa agcgatcttc tcacggtggc cggtctcggc
    10621 gagccgcaac gtcgccgacg cggcatagtc cgggtcggcg acgcgggctt ccggcacgca
    10681 ggcggcgatg cggatgaatt cgtggctata gagcgaagag aacgccgcca tcgcggtgaa
    10741 cctttccgtt caaaagtcgc tttgccgcag cgaagcgcgg agggaccgta aggcttgcgg
    10801 ctttgatttt caaagccggc cggcggatcg ccacagagcg gcgccggcgg aggcgatcgg
    10861 cggagattga tcgcggggct cggagaggct agttacgccg ccctggcgac gaggccttct
    10921 atgctctcct ggctttccta cgccgcgagc ccgattcacg tcctgctggc cctctgggtc
    10981 acctgtcacg ttctgcttca caaacgggac gtacgcgccg ccatcggctg gatcggcacg
    11041 gcgtggctcg cgcccattgt cggcgcactg ctctatttcg ctttcggcat cagccgcgtg
    11101 aagcggcgcg cccgccgcct gatgggggcc aatcgcgcga tcgacccttc gctcggacgg
    11161 gacgtttctt ccgacgatcc ggtcgagcgg ctcaaaatcg ccatcagccg aatcaccggc
    11221 gaagagatca gcgccggaaa agtggtcgcc attttggaca gcggcgacga ggcctatccg
    11281 catatgctcg cggcgatcga cgcggcaaag accagcgtcg atctctcgac ttacatattt
    11341 cgcaccgact ttgtcgggac gaaattcatc gccgcgctga cccgcgctcg ccagcggggc
    11401 gccaccgtgc gcgtcctcat cgacggcttc ggcggcggct tcctgctctc gccggcctat
    11461 cgcgctctgc gcaagaacgg cgttccggcc gcccggtttc ttcattccgc gctgccctgg
    11521 aaaatgccgc tactcgacct tcgtctgcac aagaagaccc tgatcgtcga tcgcgccgcc
    11581 gcctatatcg gcggcttgaa catcggcgct gaaaactcca acgaaaagcc ggccaaaaaa
    11641 gccaccgtgc gggacctgca ttttcgcgtc gaggggccgg tcgttcacca aatcgaagag
    11701 gatttcgacg aggattggac attcgccacc ggagagccgc cggcaatgac cccgcgccag
    11761 cccgatattt cggcgagcgg cgacgctgcg cgcgtcatcg tcgccgggcc cgatcaggat
    11821 gaagaccaac tcgcccttgt ccttttgtcg gcgatcaatt cggcgcagca ttcgattcgc
    11881 atcgcgacgc cctatttcct gcccgacgaa tcgctggtca ccgcgctgca actggccgcc
    11941 ttgcgcggcg tcgacgttca aatcgctttg ccggccgtca acaatcactt gctcgtcgcc
    12001 tgggcgtcgc ggccgcatat cgagccttta ctccagaccg gctgtcggat atggcgtgga
    12061 ccgccgccct tcgatcacag caagctgatg acgatcgacg gcaaatggag cctaatcggc
    12121 agctcgaact gggacgttcg cagcctgcgc ctcaatttcg agatcgcgtt ggagtgtcac
    12181 ggcgccgatc tggcgcgccg attaagcgag cttttcgatc ggaagcgaat tcagccggtg
    12241 accttgcaag aaatccagaa tcgctccgtc attctcaagc ttcgggacgc cgccgcccgc
    12301 cttatggcgc cctatctgtg aaaggcggca atccgctcat gaaaatcgct tcctacaaca
    12361 ttcacaaatg ccgaggcgtc gacggtcggg ttcgccccga ccggatcgtc tcggtgatcg
    12421 gcgaaatggc ggctgatctg gtcgccttgc aggaggtcga cggtcgcttc ggccgccgcg
    12481 gcggattgct cgatccggcg gcgatccggc gcgaaaccgg catgcatctt ctggtgcagt
    12541 cggacgcgcc tgaccggcat ggctggcacg gcaacgcctt gctcgttcgc ggcgagccga
    12601 aatcctaccg gcgggcgcgg ctgaagctgc cgggattcga gccgcgcggc gccatcgtcg
    12661 ccgagatcga tctcggcgaa ggcgaattcc gcgtcatcgc cgctcatttt ggcttattcc
    12721 gtcgctcgcg gatggaccag gcgctggcct tgctcaccgc tttcaacgat ctgccgccga
    12781 tgaacacgat ccttctcggc gatctcaacg aatggcgccg ccgccggcgc tcggcgctcg
    12841 aaatcctgga gccgaccttc ggtccggccc cgacagtctt aagcttcccc gcccgacggc
    12901 cggttttccc gctcgactgc attttcggct ggccgggtgg gttgatcggc gagatcgcgg
    12961 cgcatgacac gccgctggcg cgtcgggcgt cagaccacct gcccctgacg gcgagggcgc
    13021 atctcaaagt tcggcagccg attctgcgcg aggcggcgta agcgcttcgg cgcctgcttt
    13081 gcggtcgatt tcagggtaag gatgcatggc gcccgcccgc cagaatttcc cactgaactt
    13141 ttgtcgaaat tgacggttat tgggcgcgcc ggctctccga cgaaggtgag tgatggacac
    13201 gcgcttttcc cgttattgga cctggtcgga agctttcgag ttgctcgccc gcgccgaccg
    13261 catgcaccgg cagtttttcc ggccggtcgg ctcgccctcg ccggtcgcct gggagccccc
    13321 ggtcgacgtc atcgagacgg agcgcgccgt cttggttctc gtcgccttgc ccggggtcga
    13381 tctcggcgag gcgaaagcct cgctcgaagg cgacgaaatg gttttttccg gcatgcggga
    13441 attcccgccg gagatgcaga ccgccgccat tcatcggctg gagcttccgc aaggccgctt
    13501 cgaacggcgc gtccgccttc cgcccggccg ctatgcgtct gtccgccgct ccgagtcgaa
    13561 aggctgtctt ctgatcacat tggaaaaagc aggagccttc aatggctgac gccgatctta
    13621 aggacgcgtc gtcagacaat gccggcgcgg gcgccggcgt ctcgccgccg ctccccaccg
    13681 acgcgctcat catcctgccg gtgcgcggcc tggttctttt tcccggcatg gtcctgccga
    13741 tcgcgctcgg ccggccgcgc tcgattcttg cggcgcaacg agcggtgcgc gagcagcggc
    13801 cggtcggcgt gctgatgcag cgcaacgcgg aggctgagga ccctatcccc gtcgacatgc
    13861 atcggatggg aaccatcgcc aatgtcgtgc gctacgtgac gacgccggac ggcgcccacc
    13921 atctggtctg ccagggcgag cagcgttttc gcatcgtcga ttatctgaac ggctggccgt
    13981 tcttcgtcgc gcgcgcggaa aggatatcgg agccttcggc gctctccgcc gacatcgagg
    14041 cgcgcttcgt ccacctgaag ggccaggcgc tcgaagccct gcagctcatc ccgcaagcgc
    14101 cgccggagct ttccgccgcc attcagaacg ccgagtcgcc ggccgcgttg tcggatatgg
    14161 tcgcgacctt catggacgcg acgtccgacg agaagcagga aattcttgaa acgatcgata
    14221 tcgttccgcg cctcgatcgg gtctcccgcc tcctcgccca tcggatcgaa atcctgcgcc
    14281 tgtcgcagga gatcgggcga gagaccaagg cttcgcttga cgcgcgcaac cgcgaattcc
    14341 tgctgcgcga gcagatggcg gcgatccagc gccagctcgg cgagaccgac gaaggcaagg
    14401 gcgcggaaat cgccgagctc gaaaaggcga tcgccaaagc caatatgccg aaagaggtcg
    14461 aggaccaggc gcgcaaggag ctgaaacgcc tgcagcgcat gccggaagcg gccggcgaat
    14521 acggcatggt caggacctat ctcgactggc tgatcgaact gccctgggct ttgccggaag
    14581 aagcgcccat cgacatcgcg gcggcgcacc gcattctcga cgaggaccat ttcggactcg
    14641 aaaagatcaa gcgccgcatc gtcgaatatc tcgccgtgcg caagctcgcg cccggcggca
    14701 aggcgccgat cctgtgcttc gtcggaccgc ccggcgtcgg caagacctcg ctcggccaat
    14761 cgatcgcccg ggcgatgaac cgcaaattcg tgcgggtgag ccttggcggc gtgcatgacg
    14821 aagcggagat tcgcggccac aggcgcacct atatcggcgc gctgccgggc aatatcatcc
    14881 aggcgatccg caaagccggc gcgcgcaatt gcgtcatgat gcttgacgaa atcgacaagc
    14941 tcggcgccgg cattcagggc gatcccggct cggctctgct tgaagtgctg gaccctgagc
    15001 agaacaatac gttccgcgac aactatctcg ccgttccgtt cgatctcagc cgcgtcgtgt
    15061 tcatctgcac ggcgaacatg ctcgatacga ttcccgggcc gctgcgcgac cggatggaga
    15121 tcatcagcct cgccggctac acggcgagcg agaagctgca aatcgcgcgg cgctatctcg
    15181 tcgcgcgcca attggaggcg aatgggctca agcccgatca ggtcgccatc gccgacggcg
    15241 tgctggaaac catcattcag cattatacgc gcgaggcggg cgtacgaaat ctggagcgcg
    15301 aaatcggccg agtgctgcgg caagcggcga tgcggatcgc cgagggcgag cgcggctcga
    15361 tcgcgattgc atccgccgat ctcgcgacca ttctcgggcc gccgcctttc gagaacgaag
    15421 tcgctatgcg caccagcgtt ccgggggtcg ccaccggcct cgcctggacg ccggtcggcg
    15481 gcgacatcct gttcatcgaa gcgacggcga cgccgggcaa tggccgtttg atcctcaccg
    15541 gacagctcgg cgaggtcatg cgcgagagcg cgcaagccgc gctcagcatc gtcaagaacc
    15601 gcgccgtctc ttttggaatc gatcccaatc gctttgaaaa aaccgacatc catatccatg
    15661 tcccggccgg cgcgacgccg aaggacgggc cgagcgccgg cgtcgcgatg ttcatggctt
    15721 tggtttcgct tctgaccgaa aagaccgtgc gcagcgatac cgcaatgacc ggcgagatca
    15781 gcctgcgcgg cctcattctg ccggtcggcg gcatcaagga aaaagtcgtc gccgcgcatc
    15841 gcgccggcat caagcgcatc atgctgccgg ctcgcaacca caaggattac gaagagattc
    15901 cggaagaggt tcgcgagcag atggagttcg tctggctcga acgcgtcgat gacgcggcgg
    15961 cgcaagcgct cgaaccggcg gcgccggaaa acacggcgcg gaagctggcg tagctgaaac
    16021 cgtcatggtc tttgccggcg cgggcggctc gcggagcgac ggcatccaaa tcgccgctat
    16081 gcgctacacc gccagcccgt tctccgcagc cagatcggcc aggcgcagtt gcggccgggc
    16141 gccgagatgc tggatgatct cggccgcggc gagcgcgccg agcctggccg atttcacgtg
    16201 gccggcgccg cgcgccatgc cggcgagaaa gccggcggcg aaaagatcgc cggcgccggt
    16261 cgtatcgata agccgatcga tcgcgtaggc cggcacttcg aacgtctctt tcccgtcgac
    16321 gacgatcgag cccttctccg accgcgtcac gacgccgagc gcgccttccg cgcgcagggc
    16381 ttccaacgcg gtcttaaaat cggcggtctg gtacagcgaa tgaacctcct cggtattggc
    16441 gaagaccgta tcgacgaggc cgctgcggat gagatcaagg aattccgcgc ggaagcgatc
    16501 gacacagaag gaatccgaca gggtcagcgc aacgcgcgcg ccggcgccat gagcgagacg
    16561 cgcggctttc tggaacgtct ccttggccgc ctgcctgtcc cacagatagc cttcgaggta
    16621 gacgatgcgc gcttcgcgca ccaaattctc atcgagatca ggcggcgaga ggtgctggct
    16681 cgcgccgaga taggtgttca tggagcgctc gccgtccggc gtgacgaaaa tatagcagcg
    16741 tcctgtcgcc ggcccattgg cggcgggctt caccgagaag ccgacgccgg tcgcgcggat
    16801 gtcgtggatg aaggcgcggc cgagttcgtc gtctttcact ttgccgatat aagcggcgct
    16861 gacgcc
//
LOCUS       DATGJW010000329         3590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1512295, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000329 DATGJW010000000
VERSION     DATGJW010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3590
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512295"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..909
                     /locus_tag="VKU03_10170"
     CDS             <1..909
                     /locus_tag="VKU03_10170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HLJ71674.1"
                     /translation="FALGDVEAAVFPRSAVKALQALPLIESGAADKYKLTDAEIALAC
                     SSHSGEPIHLETARAMLAKCGLDGSALECGAHWPLDAAASRALAASGAAPSALHNNCS
                     GKHAGFLCLACAAGRATKGYVKYDHDAQRQVRAALGEMTKLDLGAAPWGIDGCAVPTY
                     AMPLRALALAFARFGAGEGLDADRAAAARRIRAACAAHPHLVAGTGRFDTLIMIALGA
                     RAFVKTGAEGVYCGALPELGLGVAIKCDDGAGRAAEVAMAAIIARFLPLDDNQRALLA
                     PRLSSVLYNWNGASVGTLRASQTLSA"
     gene            1027..1860
                     /locus_tag="VKU03_10175"
     CDS             1027..1860
                     /locus_tag="VKU03_10175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018259686.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLJ71675.1"
                     /translation="MARPVVVAVAITGSVPRKKDNPALPTSPAEQIESTHQAYEAGAS
                     LVHIHVRNPDETSSSDPALFAEVMAGVRRHCPGMIVQFSTGGRGRDQAARGSALPLKP
                     DMASLATGSVNFPLAVYENAPALVEDLAGRMRNYGVKPEIEIFDLSHIHAAGRLVESG
                     LIGESPHVQFVLGVKNALPCEERLVDILLDETRRVIPKATWTAAGIGRFQSKVIEWVL
                     SRGGEALRTGLEDNIRISKERLAKSNAELVQVAVGLCERHGARAATPAEARRMLGLAA
                     M"
     gene            1918..2631
                     /locus_tag="VKU03_10180"
     CDS             1918..2631
                     /locus_tag="VKU03_10180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509766.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit A"
                     /protein_id="HLJ71676.1"
                     /translation="MSKVYKNADEALAGVIKDGMTLMAGGFGVCGVPDSLIRAVRKSG
                     AKNLTFVSNNAGVDGGGLGILLETRQIKKMISSYVGENKLFAQQYLAGELELEFNPQG
                     TLAERIRAGGAGIPAFFTKTGVGTVIAEGKETRVFDGETYIMERGLVADIALVHAWKA
                     DTEGNLVFRKTARNFNPNMATAAKLTIAEVEYLVPTGEIDPDHIHTPGIFVQRIVHVP
                     VALKDKVIEHRTTRKRPAA"
     gene            2773..3420
                     /locus_tag="VKU03_10185"
     CDS             2773..3420
                     /locus_tag="VKU03_10185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CoA transferase subunit B"
                     /protein_id="HLJ71677.1"
                     /translation="MAWTRDQMAARAAKELRDGFYVNLGIGIPTLVANFIPPGVNVVL
                     QSENGMLGMGPFPYEGEEDPDLINAGKQTVTTLPTTSFFSSADSFAMIRGGHIDLAIL
                     GAMQVAENGDLANWMIPGKMVKGMGGAMDLVAGVKKVVVVMEHTAKDGPKLLHKCTLP
                     LTGVRVIDMVITDLGVFTIDKHGSGGMKLIELADGVTQEEIRAKTEANYQVALAA"
BASE COUNT          628 a         1178 c         1201 g          583 t
ORIGIN      
        1 ttcgcgctcg gcgatgtcga ggcggcggtc tttccgcgct cggcggtcaa ggccttgcag
       61 gcgctgccgc tgatcgagtc tggcgccgcg gataagtaca agctgaccga cgcggagatc
      121 gcgctcgcct gctcgtcgca ttccggcgag cccatccatc tcgagacggc gcgcgccatg
      181 ctcgccaaat gcggcttgga tggaagcgcg ctcgaatgcg gcgcgcattg gccgctcgat
      241 gcggcggctt cccgcgcgct cgccgcgtcg ggtgcggcgc cgagcgcgct ccataacaat
      301 tgctcgggca agcatgccgg cttcctctgc ctcgcctgcg ccgccggccg cgcaaccaaa
      361 ggttacgtca aatacgatca cgacgcgcag cgccaggttc gcgccgcgct cggcgaaatg
      421 acgaagctcg atctcggggc ggcgccctgg gggatcgacg gctgcgcggt cccgacttac
      481 gccatgccgc tcagggcgct cgccctcgcc ttcgcgcgct tcggcgccgg cgaggggctc
      541 gacgccgacc gcgccgcggc ggcgcggcgc atccgcgccg cctgcgccgc ccatccgcat
      601 cttgtcgcag ggaccggccg cttcgatacg ctgatcatga tcgcgctcgg cgcccgcgct
      661 ttcgtcaaga cgggcgccga aggcgtctat tgcggcgcgc tgccggagct tgggcttggc
      721 gtcgcgatca aatgcgacga cggggctgga agggccgccg aagtcgcgat ggccgccatc
      781 atcgcccgtt tcctgccgct ggacgacaat cagcgcgccc tcctggcgcc gcggctgtcg
      841 tccgtgctgt acaactggaa tggcgcgtcg gtcggaacgc tgcgggccag ccagacgctg
      901 agcgcttgac ccaggcgcgg gcattttggc gcggccgtcc cggctaccca acccgccggc
      961 aatccgttag aacccctgca aggcgctttc gcaactgcga agccctcttc atccaagcga
     1021 ggcgcaatgg ctcggccggt tgtcgtggcg gtggcgatca cgggctcggt gccgcgcaag
     1081 aaggacaacc cggcgctgcc gacatcgccg gcggagcaga tcgagtccac tcatcaagct
     1141 tacgaggccg gcgcttcgct ggtccatatc catgtccgca accctgacga gacgtcatcc
     1201 tccgacccgg ctttgttcgc cgaagtgatg gcgggcgtgc gccgccattg cccgggcatg
     1261 atcgtgcaat tctccaccgg cgggcgcggc cgcgaccagg cggcgcgggg cagcgccctg
     1321 ccgctgaagc cggacatggc ctcgctcgcc accggctcgg tcaattttcc cctcgccgtc
     1381 tacgagaacg cgcccgcttt ggtcgaggat ctcgccggcc ggatgcgcaa ttacggcgtc
     1441 aagccggaga tcgagatttt cgatctctcg catatccacg ccgccggccg cctcgtcgaa
     1501 agcggcctga tcggcgagag cccgcatgtg caattcgtgc tcggcgtcaa gaacgcgctg
     1561 ccttgcgagg agcggctggt cgatattctc cttgacgaga cccggcgcgt catcccgaaa
     1621 gcgacctgga cggccgccgg catcggccgc ttccagagca aggtcatcga atgggtgctg
     1681 tcgcgcggcg gcgaggcgct gcgcaccggg ctcgaagaca atatccggat cagcaaggag
     1741 cgcctggcca agagcaatgc ggagctggtt caggtggcgg tcgggttatg cgagcgccac
     1801 ggcgcaaggg cggcgacgcc cgccgaggcg cggcgaatgc tcggccttgc ggcgatgtga
     1861 ggcggaggac gcccgacgcg gatgatcacg tgaggcgaca tcgatgagga tgaacagatg
     1921 agcaaagtct ataagaatgc cgacgaggcg ttggccggcg tcatcaagga cggcatgaca
     1981 ttgatggcgg gcgggttcgg cgtttgcggc gtgccggatt cgctgatcag ggcggtccga
     2041 aaatccggcg ccaagaacct gaccttcgtg tccaacaatg ccggcgtcga tggcggcggc
     2101 ctcggcattc tgctcgagac ccggcagatc aagaagatga tctcgtccta tgttggcgag
     2161 aataagctct tcgcgcagca atatctggcc ggcgaattgg agctggagtt caatccgcaa
     2221 ggcacgctgg ccgagcgcat ccgcgccggc ggcgccggca ttccggcctt cttcaccaag
     2281 accggcgtcg gcacggtgat cgccgagggc aaggagacgc gcgtcttcga cggcgagacc
     2341 tacatcatgg agcgcggcct cgtcgccgac atcgctctgg tccatgcctg gaaggcggac
     2401 accgaaggca atctcgtctt ccgcaagacg gcgcgcaatt tcaatccgaa catggcgacg
     2461 gcggccaagc tgaccatcgc cgaggtcgag tatctcgtgc cgacgggcga gatcgatccc
     2521 gaccatatcc atacgccggg cattttcgtg cagcgcatcg tgcacgtccc ggtggcgctg
     2581 aaagacaagg tcatcgagca ccgcacgacg cgcaagcggc cggcggcgta aggatgtacg
     2641 agacatgcac cgccgtcatt gcgagcgaag cgaagcaatc cactcgtagg gccgcgcccg
     2701 gtgaatggat tgcttgtcgc ttcgctcctc gcaatgacag cccatacgga tcaaaaatca
     2761 gaggctagac ccatggcatg gaccagagac caaatggccg cccgcgccgc caaggagctg
     2821 cgcgacggct tctatgtcaa tctcggcatc ggcattccga cgctggtggc gaatttcatc
     2881 ccgccgggcg tcaacgtcgt cctgcagagc gagaacggca tgctcggcat ggggccgttc
     2941 ccctatgagg gcgaggagga tcccgacctg atcaacgccg gcaagcagac ggtcaccacc
     3001 ttgccgacga cgagcttctt ctccagcgcc gattctttcg cgatgattcg cggcggccat
     3061 atcgatctcg ccattctcgg cgcgatgcag gtcgccgaga acggcgacct cgccaattgg
     3121 atgatccccg gcaagatggt caaaggcatg ggcggcgcca tggaccttgt cgcgggcgtg
     3181 aagaaggtgg tcgtggtgat ggagcacacg gccaaggacg ggccgaagct gctgcacaaa
     3241 tgcaccttgc cgctgaccgg cgtccgcgtc atcgacatgg tcatcaccga tctcggcgtg
     3301 ttcacgatcg acaaacacgg ttcgggcggc atgaagctga tcgagctcgc cgacggcgtc
     3361 acgcaggagg agattcgcgc gaagacggaa gcgaactacc aggtcgcgct ggccgcgtag
     3421 cgcctcatcc tcccccgtgg agcgaagcga aacgggggag gggaaccgcg cgtcagcgcg
     3481 gtggaggggg ccgcggcggt tgggattatc cttgagagac cggagccctc ctcctccacc
     3541 atgcttcgga tggtcccccg ctttcgcggg ggaggaaagg gcctccctca
//
LOCUS       DATGJW010000330         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_994522, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000330 DATGJW010000000
VERSION     DATGJW010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_994522"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            38..229
                     /locus_tag="VKU03_10190"
     CDS             38..229
                     /locus_tag="VKU03_10190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020185432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2065 domain-containing protein"
                     /protein_id="HLJ71678.1"
                     /translation="MRDFSAAVGLVFAIEGLLLAGFTETMRERMAQAAKTDSKRLRVL
                     GLVAALIGVAIVWAARRIL"
     gene            310..1821
                     /locus_tag="VKU03_10195"
     CDS             310..1821
                     /locus_tag="VKU03_10195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegQ family serine endoprotease"
                     /protein_id="HLJ71679.1"
                     /translation="MRLAPSPHSLAGRRGRGIALALLLGAGAPLGFGVAADLAAGAPA
                     LAQAKPSNLADLVESVADAVVNISATQTVEDKSAENMPQLPHGTPFDEMFEEFFKNHG
                     QLGPMPRHKTSSLGSGFVIDPSGIIITNNHVIADANDITVIFTDGRKLKAQIVGKDPK
                     VDVAVLKVKTDKPLKAVKFGDSDKMRVGDGVFAVGNPFGLGETVTAGIVSARNRNIDS
                     GPYDNFIQTDAAINKGNSGGPLFNMAGEVIGINTAILSPSGGSIGIGFATPSATVQPV
                     IAQLEQFHETRRGWLGVRIQNVDDQIADSLGLGQARGALVAGVDEKGPAKPAGIEAGD
                     VIVKFNGKDIKESRDLPKIVASTPVGTEVEVVVIRKGQEITKRVTLGRLEDGEKAEAT
                     SKSDEAPADKAINTHIFGMELSGLSPDLRKSFQIKDNVKGVVVSSVDPNSPAAEKGLR
                     PGDVIQEVNQVAVADPKDVSQAIDAAKKAGKKTALLLVSNAQGDARFVAMAVQ"
     gene            complement(1889..>2018)
                     /locus_tag="VKU03_10200"
     CDS             complement(1889..>2018)
                     /locus_tag="VKU03_10200"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012254948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="L-serine ammonia-lyase, iron-sulfur-dependent,
                     subunit alpha"
                     /protein_id="HLJ71680.1"
                     /translation="GDGSHKVSLDSVIATMRQTGADMQSKYKETSLGGLAVNFVEC"
BASE COUNT          378 a          675 c          647 g          318 t
ORIGIN      
        1 gccgcccgcc gccgccgcgg ctgccgcgag ccactagatg cgcgactttt ccgcagcggt
       61 cggcctggtc ttcgccatcg aaggcttgct gctcgccggt ttcaccgaga cgatgcggga
      121 aagaatggcg caagcagcca agaccgattc gaagcgcttg cgcgtgctcg gtctcgtcgc
      181 ggcgctgatc ggcgtcgcca tcgtctgggc ggcgcggcgg attctttagc ggccgcccgt
      241 cgcattgccg ctgttgcgcc ccaatttgtg gtcaggggat cgccccagcc gattcaggag
      301 tgacagatta tgcgcctcgc cccttcgccc cactcgctcg ccggtcggcg agggcgcggc
      361 atcgcgctcg cgctcttgct tggcgccggc gctccgctcg gcttcggcgt cgccgccgat
      421 ctcgccgccg gcgcgccggc tttggcgcaa gcgaagccga gcaacctcgc cgaccttgtc
      481 gagagcgtcg ccgatgcggt cgtcaacatt tcggcgaccc aaaccgtcga ggacaagagc
      541 gccgagaaca tgccgcaatt gccgcatggc acgcccttcg acgaaatgtt cgaagagttc
      601 ttcaagaatc acggccaact gggcccgatg ccgcggcaca agaccagttc gctcggctcc
      661 ggctttgtca tcgatccgtc aggcatcatc atcaccaaca atcacgtcat tgccgacgcg
      721 aacgacatca cggtgatctt caccgacggc cgcaagctca aagcgcaaat cgtcggcaag
      781 gacccgaagg tcgacgtcgc ggtgctgaag gtcaaaacgg acaaaccgct gaaagcggtc
      841 aagttcggcg acagcgacaa gatgcgggtc ggcgacggcg tcttcgccgt cggcaatccg
      901 ttcggcctcg gcgaaaccgt gaccgccggc atcgtttcgg cccgcaaccg caacatcgac
      961 agcgggccgt acgacaattt catccagacc gacgccgcga tcaacaaggg caattccggc
     1021 gggccgctct tcaacatggc cggcgaggtg atcggcatca acacggcgat cctgtcgccg
     1081 tcgggcggct cgatcggcat cggctttgcg acgccctccg ccacggttca gccggtcatc
     1141 gcccaactcg aacagttcca tgagacgcgg cgcggctggc tcggcgtgcg catccagaat
     1201 gtcgacgacc agatcgccga cagcctcggc ctcggccagg cgcgcggcgc gctggtcgcc
     1261 ggagtcgacg agaagggccc ggccaagccg gccggcattg aagccggcga cgtgatcgtc
     1321 aagttcaacg gcaaggatat caaggaatcg cgcgatctgc cgaagatcgt cgcctcgacg
     1381 ccggtcggga ccgaagtcga ggtggtcgtc atccgcaaag gtcaggaaat caccaagcgc
     1441 gtgacgctgg gccggctcga agacggcgag aaggcggaag cgacgagcaa gagcgacgag
     1501 gcgcccgccg acaaggcgat caacacgcat attttcggca tggagctttc cggcctctcc
     1561 cccgaccttc gcaagagctt ccagatcaag gacaatgtga agggcgtcgt ggtgtcgagc
     1621 gtcgatccga attcgccggc cgccgaaaaa ggcctgcggc cgggcgacgt catccaggag
     1681 gtcaaccaag tcgcggtggc cgatccgaaa gacgtgagcc aggcgatcga cgcggccaaa
     1741 aaggccggca agaagacggc gctgctgctc gtctccaacg cgcaaggcga cgcgcgcttc
     1801 gtcgcgatgg cggtgcagta ggcctgtagc cgcgctcggc gtctgtggac gcggtcgccg
     1861 accgcgtcta cagggtgacg cgccccgttc agcactcgac gaaattcacc gccagcccgc
     1921 cgagcgacgt ctccttgtat ttcgactgca tgtcggcgcc ggtctggcgc atcgtcgcga
     1981 tgaccgaatc gagagagacc ttgtggctgc cgtcgcca
//
LOCUS       DATGJW010000331         2192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_580616, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000331 DATGJW010000000
VERSION     DATGJW010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2192
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580616"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            106..840
                     /locus_tag="VKU03_10205"
     CDS             106..840
                     /locus_tag="VKU03_10205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015688792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71681.1"
                     /translation="MDNVLVTGASRGIGLGIAKKLAACGYRVIAVARRESAELAAAAA
                     EAERSAAGAMRFEAFDLGEIDAISGFVAALRKRHGPLYGLVNNAAIGTEGLLANMPNA
                     EIEALLRLNTLSPIVLTKYAVRGMMAEGRGRIVNLASIVASTGYNALSVYAATKASMV
                     GFTKSLAREVGRVGVTVNAIAPGFIDTAMTEGLAADQRKRIMDRSALRRLAEVEDVAN
                     MAAFLIGETGRNITGAVMTVDAGNTA"
     gene            complement(1262..2185)
                     /locus_tag="VKU03_10210"
     CDS             complement(1262..2185)
                     /locus_tag="VKU03_10210"
                     /EC_number="6.2.1.n2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012030362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--[acyl-carrier-protein] ligase"
                     /protein_id="HLJ71682.1"
                     /translation="MTIALKTLGQPKTAPEALLETLFRPMGIDGVYGRTGRYEDVVDG
                     LSALISRHRPDRAEIFRFPPVVSRAMIEKSGYLKSFPHLLGCVCALQGGEREIGAAVD
                     RFVAGEDWTGALKTADLVLAPAACYPVYPLAAARGALPAEGYVFDVACDCFRREPSRD
                     IDRLQSFRMREFVRIGTPDQVRAFRQDWMTRATAIAETLGLVYRVEAASDPFFGRGGV
                     LVSKAQAQQELKFELVVPVRSAEKPTACMSFNYHRDHFGQTWAMKTEAGEAAHTACVA
                     FGMDRLAVALFATHGIDPAQWPKTVRQALAL"
BASE COUNT          414 a          701 c          703 g          374 t
ORIGIN      
        1 ccattctcgc ggcctgccgc gccgaacttg ccccctataa gaccccggcc gtcatccgct
       61 tcgtcccgaa gttggcgatg accatggccg gaaagctggc gcgcgatgga taatgttctt
      121 gtgaccggcg cgagccgcgg catcggattg ggaatcgcca aaaagctcgc cgcttgcggc
      181 tatcgcgtga tcgcggtggc gcgccgcgaa agcgccgagc tcgccgcggc ggccgccgag
      241 gccgaaagga gcgcggccgg cgcgatgcgc ttcgaagctt tcgatctcgg cgagatcgac
      301 gcgatttccg gctttgtcgc cgccttgcgc aagcggcacg gcccgcttta cggccttgtc
      361 aacaatgcgg cgatcggcac ggaaggcctg ttggccaaca tgccgaacgc cgagatcgag
      421 gcgctgctgc ggctcaacac gctctcgccg atcgtcctga cgaaatatgc ggtgcgcggc
      481 atgatggccg agggccgcgg ccggatcgtc aatctcgcct cgatcgtcgc ctcgaccggc
      541 tacaatgcgc tgtccgtcta cgccgcgacc aaggcctcaa tggtcggctt caccaaatcc
      601 ctagcgcgag aagtcggccg cgtcggcgtc accgtcaacg ccatcgcgcc gggcttcatc
      661 gacaccgcga tgacggaagg gctcgcggcc gatcagcgca agcggatcat ggaccggagc
      721 gcgctgcgcc gcctggccga ggtcgaggac gtcgccaata tggccgcctt cctgatcggc
      781 gaaaccgggc gcaacatcac cggcgcggtg atgacggtgg acgcgggcaa cacggcgtag
      841 cgcgccgcgc acagaggcgc caactcacgc gctccgctta ggcgataaaa agtacgatcg
      901 tccgggattt cgatctcaat tcatggtcat gtcatggaag atcaaacaat ttcttcttcc
      961 aaggatcaat ttcggatata attcgcgtcg agacccggag cgcgtatgtc gccgcggctg
     1021 aacggatcaa aaaacgcatt ttcaattttt tttcgctcca attcattgaa attgctcgaa
     1081 acggccaaag aaaaatttgg aaatgcttgg cggacgcctt gagcggaccg ctgatgacaa
     1141 agggtatttt cgaacgcgga tttgccggat cggcgtcttt tcgcgcgatc caaggatttt
     1201 ccaaatttgc ggcgaagccg ggctgacgcc ggcgatcggt gtcgcgaggc gcggcggaca
     1261 gctacaacgc cagcgcctga cgcacggttt tcggccattg cgccggatcg attccatgcg
     1321 ttgcgaacaa ggcgaccgcc agccggtcca tgccgaaggc gacgcaggcc gtatgggcgg
     1381 cctcgcccgc ctcggtcttc atcgcccagg tctggccgaa atgatcgcgg tggtagttga
     1441 agctcatgca ggcggtcggc ttttccgccg agcggaccgg cacgacgagt tcgaacttca
     1501 attcctgctg cgcttgcgcc ttgctgacca gcacgccgcc gcggccgaag aacggatcgc
     1561 tcgccgcctc gacgcgatag accaggccga gcgtctcggc gatcgccgtc gcgcgggtca
     1621 tccaatcctg gcggaaagcg cggacctgat cgggcgttcc gatgcgcacg aattcgcgca
     1681 tccggaagga ctgaagacgg tcgatgtcgc gcgacggctc gcgccggaag cagtcgcacg
     1741 cgacgtcgaa gacatagcct tcggccggca gggcgccgcg cgccgcggcg agcggataga
     1801 ccggatagca ggcggccggc gcgaggacga ggtcggcggt cttcaatgcg ccggtccaat
     1861 cctcgccggc gacgaaacga tccaccgcgg cgccgatttc gcgctcgccg ccttgcaagg
     1921 cgcagacgca gccgagcaga tgcgggaagc ttttcagata gcccgacttc tcgatcatcg
     1981 cgcggctgac gaccggcggg aagcggaaga tttcggcgcg gtccggacgg tggcgcgaaa
     2041 tcaaggccga gagcccgtcg acgacgtcct cgtaacgccc cgtgcggcca tagacgccgt
     2101 caattcccat cggacggaac aacgtttcaa gcagcgcttc cggcgcggtt ttcggctggc
     2161 cgagagtctt caacgcaatt gtcatcgcag ca
//
LOCUS       DATGJW010000332         4931 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_580647, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000332 DATGJW010000000
VERSION     DATGJW010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4931)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4931)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4931
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_580647"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1198
                     /locus_tag="VKU03_10215"
     CDS             <1..1198
                     /locus_tag="VKU03_10215"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018405999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HLJ71683.1"
                     /translation="DPIFRHFFAEQGGPARVAQSLGSGVIVDRSGLIVTNNHVIEGMT
                     DVKVALADHREFEAKILLRDRRSDLAVLKIKDGGDDFPVLELGDSDAIEVGDFVLAIG
                     NPFGVGQTVTQGIVSATARTQIGANDYGFFIQTDAAINPGNSGGALVDLDGKLIGINS
                     EIISRSGGNMGLGFAIPVNMVKSVIAAAKGGSRLVRRPWLGASLQNVTQDIAESLGLD
                     KAVGALVAGVVDGGPADEAGLKRGDIVVAVDGVEVDDSGGVGFRLGVKPLGGEAKLGV
                     IRGGKKIELALRLTAAPETPPRDPVKIKTRSPFQGATLVNISPAVIEDMSLENAAKGV
                     VVAEVEEGSTAAQVGVQKGDIVIAINGQKIETTRDAERASAARVNYWRLVIQRGGQVI
                     QTVIGG"
     gene            1343..2377
                     /locus_tag="VKU03_10220"
     CDS             1343..2377
                     /locus_tag="VKU03_10220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018330152.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLJ71684.1"
                     /translation="MIFRQLFDGASGTYSYLLASRHGGEALIIDPVLEKVDRYLKLME
                     ELDLKLVKAVDTHLHADHITGLGALRDRTQCITVMGEQSKVDVVSMRIGEGDKLRVEG
                     VSLDVLYTPGHTDDSYSFVMGDRVFTGDTLLIRGTGRTDFQNGSARAQYESLQRLLRL
                     PDETLVFPAHDYKGDTVSTIGEERRCNPRLQVKGADEYIALMDNLHLPNPKMMDVAVP
                     ANMRQGFLQAEIARRGWSLTAAAARDWLGRSDVAFVDLRERAEREKHGVIPGSIHAPY
                     TELRENIHPGGVLHELAAATGKRLLFYCAFGERSAMAVQAAQDAGFASACHIEGGVSA
                     WRAAGGPCER"
     gene            complement(2430..3308)
                     /locus_tag="VKU03_10225"
     CDS             complement(2430..3308)
                     /locus_tag="VKU03_10225"
                     /inference="COORDINATES: protein motif:HMM:NF013775.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphotransferase"
                     /protein_id="HLJ71685.1"
                     /translation="MSECSRFDDEKLARAIAAAKAAARLAGDIDVRVLYRSRRVALLL
                     PAIAAVARIGPADPACLAADARELEVARHLAKSGAPVVGPSAFMPPAPIVVEDLAVTF
                     WPHTEHTSRGYDDRRAIAQAAAALRRVHERFADYAEPLPSYWERIEACGALLCRRDAL
                     PSLHRGDRAFLRKTYDRVCAALSGRDVRNVPIHGDAHMDNVFFTPAGALWTDFETACL
                     GPREWDAAALSDPAAFPALDHDLYRLLADLRSVCVIVWCSTLAHDPKKREAAREQLAR
                     LRDPRRHCARSEAIHS"
     gene            complement(3369..4514)
                     /locus_tag="VKU03_10230"
     CDS             complement(3369..4514)
                     /locus_tag="VKU03_10230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018316447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ71686.1"
                     /translation="MPYRSSWMTEELDLFRDQFRRYLHSELAPRAEGWRKDKIIDRDA
                     WRGLGGMGALLTCVPEAYGGLGATFATEAAAMEEIVAIVPELGTPVSVHSAIVAHYIL
                     NYGSQEQKSRWLPKMASGELIGAIAMTEPGAGSDLQAVRTSAKKQGNAYVLNGQKTFI
                     TNGQLADLVIVVARTGEPGAKGISLFVVETQGAEGYRRGRNLDKIGLHASDTSELFFD
                     NVSVPPDNLLGGEEGQGFAQLMQQLPQERLILAVGAVAAMESALATTIDYAKQRQAFG
                     RPIIEFQNTAFTLAERKSEAQIARVFLDWCIERHIAGELDTVTASMAKYWCTDRQMAT
                     IDDCLQLHGGYGYMAEYPIARMFVDARIQKIYGGTNEIMKMIIARSL"
BASE COUNT          850 a         1602 c         1646 g          833 t
ORIGIN      
        1 cgatccgatc ttccgccatt tcttcgccga gcagggcggc ccggcgcgcg tcgctcaatc
       61 gctcggctcc ggcgtcatcg tcgaccgctc cggcctcatc gtgaccaata atcacgtcat
      121 cgagggcatg accgacgtga aggtcgctct cgccgatcac cgcgaattcg aagcgaagat
      181 tttgctgcgc gatcggcgca gcgatctcgc cgtcttgaag atcaaggatg gcggcgacga
      241 tttcccggtg ctcgaactcg gcgattccga cgcgattgag gtcggcgatt tcgttctcgc
      301 catcggcaat ccgttcggcg tcggccagac ggtgacgcag ggcatcgtct cggcgaccgc
      361 ccgcacccag atcggcgcca atgattacgg ctttttcatt cagaccgacg cggcgatcaa
      421 tccgggcaat tcgggcgggg cgctggtcga tctcgatggc aagttgatcg gcatcaattc
      481 ggagatcatt tcgcgcagcg gcggcaatat gggcctcggc ttcgccattc ccgtcaatat
      541 ggtcaaaagc gtgatcgcgg ccgccaaagg cggctcgcgc ttggtgcggc ggccctggct
      601 cggcgcgagc ttgcagaacg tgacccagga catcgccgaa tcgctcggcc tcgacaaggc
      661 ggtcggcgcg ctcgtggcgg gcgtcgtcga cggcggcccc gccgacgaag ccggcctcaa
      721 gcgcggcgac attgtcgtcg ccgtcgacgg cgtggaagtc gacgattccg gcggcgtggg
      781 cttccgtctc ggcgtcaagc cgcttggcgg cgaggccaag ctcggcgtga tccgcggcgg
      841 caagaagatc gagctggcgc tgcgtctcac ggccgcgccg gagacgccgc cgcgcgatcc
      901 ggtcaagatc aagacgcgtt cgccgttcca gggcgcgacc ctggtcaaca tctcgccggc
      961 ggtgatcgag gacatgtcgc tcgaaaacgc cgccaagggc gtggtggtcg ccgaagtcga
     1021 ggagggctcg accgcggccc aggtcggcgt gcagaaaggc gatatcgtga tcgcgatcaa
     1081 cggccagaag atcgagacga cgcgcgacgc cgaacgcgcc tccgccgccc gggtgaacta
     1141 ttggcgcctg gtcatccagc gcggcggcca ggtgatccag acggtgatcg gcggataggc
     1201 gcgtaatggg ggaggggaat tgcagccgcg gtcggcgacc gcggctacgg cgcggcggct
     1261 gaagcatgcg gcgcatgcga tcgcttcccg cccgcgctgc gccgcgctac catgacgcca
     1321 ggagcgaatg cgggagggcg ccatgatctt ccggcaattg ttcgacggcg cttcgggcac
     1381 atacagctat cttctcgcca gccgtcacgg cggcgaagcg ctgatcatcg atccggtcct
     1441 cgaaaaggtt gaccgctacc tgaagctgat ggaggagctc gacctcaagc tggtcaaggc
     1501 cgtcgacacg catctgcacg ccgaccacat taccgggctc ggcgcgttgc gcgaccgcac
     1561 gcaatgcatc accgtgatgg gcgagcagag caaagtcgat gtcgtctcga tgcggatcgg
     1621 cgaaggcgac aagcttcgcg tcgaaggggt tagcctcgac gttctctaca cgcctggcca
     1681 taccgacgat tcctacagtt tcgtcatggg cgatcgtgtg ttcaccggcg acacgctgct
     1741 gatccgcggc accggccgca ccgacttcca gaacggcagc gcgcgggcgc agtacgaaag
     1801 cttgcaaagg ctgttacgcc tgcccgacga gaccctcgtc tttcccgcgc atgattacaa
     1861 gggcgatacg gtctcgacga tcggggagga gaggcgctgc aatccgcgcc tgcaagtcaa
     1921 aggcgccgac gaatacattg cgctgatgga caatttgcat ttgcctaacc cgaagatgat
     1981 ggatgtcgcg gtgccggcca atatgcggca gggtttcttg caggcggaga tcgcgcggcg
     2041 cggctggtct ttgaccgcgg ccgcggcgcg cgattggctc ggccggtccg acgtcgcttt
     2101 cgtcgacctg cgcgagcgcg ccgagcgcga gaagcatggc gtcatccccg gctcgattca
     2161 cgcgccctat accgagcttc gcgagaacat ccatccaggc ggcgttttgc acgaactcgc
     2221 cgcagcgacc ggcaagcgcc tgttgttcta ttgcgccttc ggcgagcgca gcgcgatggc
     2281 cgtgcaggcg gcgcaggacg ccggcttcgc ctcggcctgt catatcgagg gcggcgtcag
     2341 cgcctggcgc gccgccggcg gcccttgcga gcggtgagag gcacgtaccg tcattgcgag
     2401 cgcggcgaag cgatccgcca ggcttctctc tacgaatgga ttgcttcgct gcgcgcgcaa
     2461 tgacggcggg gatcgcggag ccgcgcgagc tgctctctcg ccgcctcgcg cttcttcgga
     2521 tcatgggcga gcgtcgagca ccagacgatc acgcaaacgc tgcgcagatc ggcgaggagc
     2581 cggtagagat cgtgatcgag cgccggaaag gcggcgggat ccgacagcgc cgcggcgtcc
     2641 cactcgcgag ggccaaggca agcggtctcg aaatccgtcc aaagcgctcc ggcaggcgtg
     2701 aaaaagacat tgtccatatg ggcgtcgccg tgaatcggaa cgtttcggac gtctcggccg
     2761 gatagcgcgg cgcatacccg atcgtaggtt tttcgaagga aggcgcgatc gcctcgatgg
     2821 aggctcggca gcgcgtcgcg gcgacaaagg agcgcgccgc acgcctcgat cctttcccaa
     2881 taggagggca gcggctcggc ataatcggcg aagcgctcgt gcacccggcg caaggcagcg
     2941 gccgcctgcg cgatcgcgcg gcgatcgtca tagccgcggc tcgtatgctc cgtgtgcggc
     3001 caaaaagtca cggccaggtc ttcgacgacg atgggcgccg gcggcatgaa cgcgctcggg
     3061 ccgacaaccg gcgcgccgct cttcgccaga tggcgcgcaa cctccaattc gcgcgcatcg
     3121 gcggcgaggc atgccggatc ggccgggccg atccgtgcga cggccgcgat cgccggaagc
     3181 agcaacgcaa cgcgccgcga gcgatagaga acgcgcacgt cgatatcgcc ggcaagccgc
     3241 gccgccgctt tcgccgccgc gatggcgcgc gccagcttct cgtcatcgaa ccggctgcat
     3301 tcgctcaaaa cgcccgccgc gattctgaaa aatgcggccg ctccaggcag gcgccgagcc
     3361 ggcccgctct acagcgaccg ggcaataatc attttcatga tctcgtttgt tccgccgtaa
     3421 atcttctgga tgcgcgcgtc gacgaacatc cgggcgatgg gatattcggc catatagccg
     3481 tagccgccgt gcaattgcag gcaatcgtcg atcgtcgcca tctgccggtc ggtgcaccaa
     3541 tatttggcca tcgaggcggt gacggtgtcg agctcgccgg caatatggcg ctcgatgcac
     3601 caatcgagaa agacgcgggc gatctgggct tcgctcttgc gctcggccag ggtgaaggcg
     3661 gtattttgga attcgatgat cggccggccg aaagcctggc gctgtttggc gtaatcgatg
     3721 gtcgtcgcca acgcgctctc catcgccgcg accgcgccga cagcgaggat cagccgctcc
     3781 tgcggcaatt gctgcatcaa ttgagcgaag ccctggcctt cttcgccgcc gagcagattg
     3841 tccggcggga cgctgacatt gtcgaagaac aattccgacg tgtcggaagc gtgcaggccg
     3901 atcttgtcga ggttgcggcc gcggcgatag ccctcggccc cctgggtctc gacgacgaaa
     3961 agcgaaatgc ccttggcgcc cggctcgccg gtacgcgcga cgacgatcac gaggtcggcg
     4021 agttggccat tggtgatgaa ggttttctgg ccgttcagga cataggcatt gccctgtttt
     4081 ttggcgctgg tgcgcaccgc ctgcagatcg gagccggcgc cgggctcggt catggcgatc
     4141 gcgccgatca attcgccgct cgccattttt ggcagccaac ggcttttctg ctcctgcgag
     4201 ccatagttga gaatgtaatg agcgacgatc gcgctatgca cgctgaccgg cgtaccgagc
     4261 tcggggacga tggcgacgat ctcctccatc gccgccgctt cggtggcgaa tgtcgcgccg
     4321 agaccgccat aggcttccgg cacgcaggtc agcagcgcgc ccatgccgcc caagccgcgc
     4381 cacgcgtcgc ggtcgatgat cttgtccttg cgccagcctt ccgcgcgcgg cgcgagctcg
     4441 ctgtgcagat agcggcggaa ctgatcccgg aacagatcga gttcctcggt catccacgag
     4501 gagcggtacg gcatgacggg tccttgggcg ctgacatttc cgccgagtct agtgcaaagg
     4561 cgcggccatg gccaaggaat cccttctccc gcgcagcgcg cgcaacgggg agaaggtggc
     4621 gtgaagcgcc ggatgagggg cggcgcagga cccgaaaggt acgcgcccgc gcttacctcc
     4681 cgaatcgctc gtcccagatt cggcgcagct cctcacccgg cgcgctccgc gcgccaccct
     4741 ctccccgcca cgctgggcga gggatcgcca aaaaaatgcc ccgccgaagc ggggctcatg
     4801 ggggacgaat cagatcggcg tcacgcggcc ttgcgggccg gcttgccctt ggccatgcgg
     4861 cgcttgatcg agctccacat cgccttcatt tccgcggcgg cggcgccggt cggatggatt
     4921 tcgctgacgc c
//
LOCUS       DATGJW010000333         7246 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_893227, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000333 DATGJW010000000
VERSION     DATGJW010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7246)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7246)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7246
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_893227"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..698)
                     /locus_tag="VKU03_10235"
     CDS             complement(<1..698)
                     /locus_tag="VKU03_10235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ada metal-binding domain-containing protein"
                     /protein_id="HLJ71687.1"
                     /translation="MDALAKSAKALAPSVESDPRWAALLARDPAAEGAFVYSVATTGV
                     YCRPTCPSRRPRPENVAFHASPADAEKAGFRPCRRCRPNEAPAAERHAALIAEACRRI
                     EKAETPPRLDALAAKAGMSPSHFHRLFKSIAGVTPKAYAAARRAERVRQELSRGESSV
                     TEAIYGSGFNASSRFYEKAKDVLGMTPSAYRAGGAEAEIRFAIGQCSLGAILVARSGK
                     GVCAILLGDDPEAL"
     gene            1178..2383
                     /locus_tag="VKU03_10240"
     CDS             1178..2383
                     /locus_tag="VKU03_10240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019170948.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyanate transporter"
                     /protein_id="HLJ71688.1"
                     /translation="MNSIRSGLQNVSRPFLLAVVILVGLNLRPFLTTIGPLTPSISEA
                     TGLGLQSMSLFTLVPMALMGVFAFVGPRLQAGVGARSAVIGALVVLCLASGLRLIASD
                     GAVLLGTAALCGLGVAIVQAVFPGIIKAHFPGRVAVVMGLYSAMLMGGGALGAQLSPV
                     VADRSGSWRIGLGWVAVPAAIALATSVLALPRERSMHAEGMGVGVLLRRPRTWLLMAC
                     FGLVNGGYASVVAWLAPYYQSLGWTSEASGRLLAAIATSQAIAALSIPALAARRLDRR
                     AWICATLLMQIAGFAGVAFWPERSTVLWAILLGAGLGGCFAVTMVVALDHFEHPGRAG
                     ALSALMQGGGFLIAAIPPWIVAVLHDASGGFVSGWLMHLGCAALVALLSLRFAPPGYA
                     RAMDASVAT"
     gene            2405..2770
                     /locus_tag="VKU03_10245"
     CDS             2405..2770
                     /locus_tag="VKU03_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006199640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIN domain-containing protein"
                     /protein_id="HLJ71689.1"
                     /translation="MAAQRDEDLFIASLTLAETRRGILEKPGGRKRDGLSAWFAGQDG
                     PQALFAGRILAFDDKAGLIWARLTAEGKAGGRPRNRLDMIIASVAAANECSIVTDDER
                     DFFGLRIVNPMRRERRLLH"
     gene            complement(2806..3678)
                     /gene="yghU"
                     /locus_tag="VKU03_10250"
     CDS             complement(2806..3678)
                     /gene="yghU"
                     /locus_tag="VKU03_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006749 - glutathione metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-dependent disulfide-bond
                     oxidoreductase"
                     /protein_id="HLJ71690.1"
                     /translation="MTDSSEYVPPKVWTWIKANGGVFANINRPVAGPTHEKELPVGRH
                     PLQLYSLATPNGVKVTIMLEELLALGRGGAEYDAWMIKIGEGEQFGSGFVAINPNSKI
                     PALVDRSGPAPIRVFESGAILMYLAEKFGAFMPSEAAARAECLSWLFWQMGAAPYLGG
                     GFGHFYAYAPTKIEYAIDRFAMEVKRQLDVLDRRLAENEYIAGSAYTIADMAIFPWYG
                     GLVKGWLYGAGEFLSVQDYKHVQRWADLLLARPAVKRGRMVNRASGDPSGQLRERHDA
                     KDFETKTQDKIAAN"
     gene            3962..5152
                     /locus_tag="VKU03_10255"
     CDS             3962..5152
                     /locus_tag="VKU03_10255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ71691.1"
                     /translation="MIARAIVLRLGLSQLICWGVSYYLIGVFGGAIAADFGWSRDLVY
                     GGFSVALLAGGLSSPLVGRLIDREGGRGVMTAGSLILAAGCLFLALSRGVASYYGAWI
                     VIGLAMRLTLYDAAFATLARIGGPEARGPMAQITLLGGLASTVLWPVGGGLAHLFGWR
                     GATIAYAGLALLTVPLHLALPRGRDGDAASASPRDAPRPLAASRADRFLAGALYALIA
                     TVANFLNSGMSAHMIGILAGLGLAASASVWIATLRGVGQSLSRGGEVLFGRRLDPLTL
                     NLAAAFVLPLAFIAGLFSARADLAALAFAFFYGAANGILTITRGTLPLILFDHRTYGA
                     FVGRLLAPSFLLSAAAPVAYAFVIEHEGAAAALDLSVALAALALAAAAILAVRFGSLK
                     RRAD"
     gene            5231..5680
                     /locus_tag="VKU03_10260"
     CDS             5231..5680
                     /locus_tag="VKU03_10260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71692.1"
                     /translation="MSRERRKRIAFLAFVIAGAWAWAAPGLAEPRLISLDDLPPDQQK
                     VVWERVDRFAEYAVILKDCVADSHFETRFVEAVKACVEPNTVRRVVGYYQQRAASLDR
                     RFSRTFCSEKKFTQNNVAQKLKSTLDNLVDYGHNLCIAYLKTGAGLR"
     gene            complement(6075..6281)
                     /locus_tag="VKU03_10265"
     CDS             complement(6075..6281)
                     /locus_tag="VKU03_10265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003528984.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CsbD family protein"
                     /protein_id="HLJ71693.1"
                     /translation="MDWNRVEGNWKQLKGKVKEQWGKLTDDDLSVINGSRDQLEGKIQ
                     ERYGIAKDQVKRDIDNWYSRQSWS"
     gene            6411..6590
                     /locus_tag="VKU03_10270"
     CDS             6411..6590
                     /locus_tag="VKU03_10270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175583.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3309 family protein"
                     /protein_id="HLJ71694.1"
                     /translation="MGTILIIILILILLGGGGGYYAHGRYGGAGLGGVLGFVLIILII
                     LWLIGAFSAGVHTPP"
BASE COUNT         1080 a         2374 c         2338 g         1454 t
ORIGIN      
        1 atcagcgcct ccggatcgtc gccgagcagg atggcgcaga cgcccttgcc gctgcgcgcc
       61 accaggatcg cgccgagcga gcactggccg atcgcgaacc tgatctcagc ttcggcgccg
      121 ccggcgcggt aggcggaggg cgtcatgccg agcacgtcct tggccttttc gtagaagcgg
      181 ctcgacgcgt tgaaaccgga tccgtagatc gcctcggtga cgctgctctc gcccctgctc
      241 aattcctggc gcacgcgttc ggcgcggcgg gcggcggcgt aggccttcgg ggtgacgccg
      301 gcgatcgact tgaacagccg gtggaaatgc gacgggctca tccccgcctt ggcggcgagc
      361 gcgtcgaggc gcggcggcgt ctccgccttc tcgatccgcc ggcaggcttc ggcgatgagc
      421 gccgcatggc gctccgccgc cggcgcctcg ttcggccggc agcgccggca tggccggaac
      481 cccgccttct cggcgtcggc cggcgaagcg tggaacgcga cattctccgg cctcggccgc
      541 cgcgacgggc aggtcggccg gcaatagacc ccggtcgtcg ccaccgaata gacgaaggcg
      601 ccctcggccg ccggatcgcg cgccagcagg gcggcccagc gcgggtcgct ttcgacggac
      661 ggggcaaggg ctttggccga cttggcgagg gcgtccatga cgaggctcat tgtagcgatt
      721 ccttcgggct gatgttgtcc tggagatagg cgctccgggc gtctctcgca ctccgcctct
      781 tgctttcaaa ttccgctgca gcgcccggcg cgcggagtgc gccgggcgct ggacaaagcg
      841 tctcctgtcg acttggcgct tcgcagccgg cgcggctggc cacgatggag cggcgttttg
      901 ccgagcttga gcggcgcgtt cataccggcg cggcggccgt tcggcaaatg caaaacgtag
      961 ttgtatcagc taattagatg ctgcgggcgg ccgccggata cccggcgtag agcggtaggg
     1021 cgcgtcgcca aggttggggt cgagggttcg atccctttcc cccgctccag ttttttggaa
     1081 atcagggcat ataaatgggg cgccggccgg cgcccgctca tctcggaaca tatcgaccat
     1141 ggcgcgtcgc gcggaagaga aaacagacga agcgggcgtg aactccataa ggagtggact
     1201 tcaaaacgtc tcccggccgt tcttgctcgc tgtcgtcatt ctggtcggcc tcaatctccg
     1261 gccgttcctc acaacgatcg ggcccctcac gccgagcatc agcgaagcga cgggtctcgg
     1321 ccttcagagc atgtcgctct tcacgctcgt cccgatggcg ctgatgggcg ttttcgcctt
     1381 tgtcgggccg cgattgcagg caggcgtcgg cgcgcgcagc gccgtgattg gggcgcttgt
     1441 cgttctttgc ctggcttcgg gcctgcggct gatcgcgtcg gacggcgccg tcttgctcgg
     1501 tacggcggct ttatgcggac tgggcgtcgc catcgtccag gccgtgtttc ccggaatcat
     1561 caaggcgcat ttccccggcc gcgtggcggt ggtgatgggg ctctattccg ccatgttgat
     1621 gggcggcggc gcattgggcg cgcaactttc gccggttgtc gccgatagga gcggaagttg
     1681 gcgcatcggc cttggctggg tcgccgttcc cgccgccatc gcgctcgcca cgtctgtgct
     1741 cgctcttccg cgagaacgtt ctatgcatgc ggaaggaatg ggcgtaggcg tcctcctgcg
     1801 ccggccgcga acatggttgt tgatggcgtg cttcggcctg gtcaatggcg gctatgcatc
     1861 ggtcgtcgcc tggctcgcgc cctactatca gtcgctgggc tggacgagcg aggcgagcgg
     1921 ccggctgctg gcggcgatcg cgaccagcca agcgatcgcg gcgctgtcga tccccgccct
     1981 cgcggcgcgg cggctggatc gccgcgcatg gatttgcgcg acgctcctca tgcagatcgc
     2041 gggctttgcc ggcgtcgcgt tttggccgga acgctcaacc gtgctgtggg cgatcctgct
     2101 cggcgccggt ctgggcggct gcttcgccgt gactatggtg gtggcgctcg accatttcga
     2161 gcatcccggc cgcgccgggg cgctctccgc gctgatgcaa ggcggcggct tcttgatcgc
     2221 cgcgatcccg ccttggatcg tggcggtcct gcacgatgcg agcggcggct ttgtatcagg
     2281 gtggctgatg catttgggtt gcgccgctct tgtcgcgctt ctcagtctgc gctttgcgcc
     2341 gcctggctat gcgcgggcga tggacgcctc tgttgcgact taggcctcat cgctgctggc
     2401 ctggatggcg gcgcagcgcg acgaggattt gttcatcgcc tcgctgacgc ttgccgaaac
     2461 ccgacgcggc attctcgaga agccgggcgg caggaagcgc gacggactca gcgcgtggtt
     2521 tgctggacag gacggcccgc aagcgctgtt tgccgggcgc attctcgcct tcgacgacaa
     2581 ggcggggctg atctgggcgc gcctgacggc ggaaggcaaa gccggcggcc gtccccgcaa
     2641 tcgactcgat atgatcatcg cgtccgtcgc ggcggcgaac gagtgctcaa tcgtaacgga
     2701 tgacgaaagg gatttcttcg ggcttcggat cgtcaatccg atgcgacgtg agcgacgtct
     2761 gcttcattga gagcattgaa ttgccgggcg gcccggatgg gggcctcaat tcgccgcgat
     2821 cttgtcctgc gtcttggtct cgaaatcctt ggcgtcgtgg cgctcgcgca attgacccga
     2881 cgggtcgccg gaggcgcggt tgaccatgcg cccgcgcttc acggccgggc gggcgaggag
     2941 caggtcggcc catcgctgaa cgtgcttgta gtcctgcacg ctgaggaatt cgccggcgcc
     3001 atagagccaa cccttcacca ggccgccgta ccacggaaag atggccatgt cggcgatcgt
     3061 gtaggcgctc ccggctatat attcgttttc ggcgaggcgg cggtcgagca cgtcgagttg
     3121 gcgcttcacc tccatcgcga aacggtcgat cgcgtattcg atcttcgtcg gcgcgtaggc
     3181 gtagaaatga ccgaagccgc cgccgagata gggcgcggcg cccatctgcc agaacagcca
     3241 ggacaggcat tcggcgcggg ccgcggcttc gctcggcatg aatgcgccga acttctcggc
     3301 gaggtacata aggattgcgc ccgattcgaa gacccggatc ggcgccgggc cgctgcgatc
     3361 gacgagcgcg ggaattttcg aattgggatt gatggcgacg aagccgctgc cgaactgctc
     3421 gccttctccg atcttgatca tccaggcgtc gtattccgcg ccgccacggc cgagcgccag
     3481 caattcttcc agcatgatcg tgaccttcac gccgttgggc gtcgccagcg aatagagctg
     3541 gagcggatgg cggccgaccg gcagctcctt ttcatgcgtc ggccccgcga cggggcggtt
     3601 gatattcgca aatacgccgc cattcgcctt gatccaggtc cagactttcg gcgggacata
     3661 ttcggacgag tcggtcatca taaggctcca gacgcagcgt tgcggcttcg cccgcagcgg
     3721 gcgattcgtt cgacgggagg aacgcgacgc ggcgcttttc gtctactcca ttctcaatcg
     3781 acgacaaaat caccaccccg cgaccgagaa tagccgcctt cgcccatcaa tttcgccggc
     3841 gcccttcctt gacgcggcgc tgcggcgggt gcgagcctcc agtgcagatt tcaagcggga
     3901 gcaatgagat gggcggctgt gcggcgcagc caattcggcg tctccggccc ggcgcgctcc
     3961 cttgatcgcg cgcgccatcg tccttcgcct cggtctttcc cagctcattt gctggggcgt
     4021 ctcgtactat ttgatcggcg tgttcggcgg cgcgatcgcc gccgatttcg gctggtcgcg
     4081 cgatctcgtc tatggcggat tttcggtcgc gcttctggcc ggcggcctgt cctcgccgct
     4141 ggtcggccgt ctcattgacc gcgagggcgg ccgcggcgtg atgacggccg gctcgctgat
     4201 cctcgccgcg ggctgcctgt ttctggcgct gtcgcgcggc gtcgccagct attacggcgc
     4261 ctggattgtc atcggtctcg ccatgcggct gacgctctac gacgcggctt tcgcgacgct
     4321 cgcccggatc ggcggtcccg aggcgcgcgg accgatggcg cagatcacct tgctgggcgg
     4381 cctcgcctcg accgtcctct ggccggtcgg cggcggcctg gcccatctct tcggctggcg
     4441 cggcgcgacg atcgcctatg ccggcttggc gctcctcacc gttccgcttc atctcgcttt
     4501 gccgcgaggc cgcgacggcg acgcggcgag cgcgtcgccg cgggacgcgc cgcggccgct
     4561 ggcggcgagc cgcgccgatc gcttcctcgc cggcgcgctt tacgcgctga tcgcgactgt
     4621 cgcgaatttt ctcaattccg gcatgtcggc gcatatgatc ggcattctcg cggggctcgg
     4681 cctcgccgcc tccgcctcgg tctggatcgc gaccttgcgc ggcgtcggcc agtcgctatc
     4741 gcgcggcggc gaagtcctgt tcggccgccg cctcgatccg ctgacgctca acctcgcggc
     4801 ggctttcgtc ctgccgctcg ccttcatcgc cggattgttc agcgcccgcg ccgatctcgc
     4861 cgcgctcgcc ttcgccttct tctatggcgc ggcgaacggc atcctgacga tcacgcgcgg
     4921 cacgctgccg ctcatcctgt tcgatcatcg gacttacggc gctttcgtcg gcaggctgct
     4981 cgcgccgagc tttctcctct cggccgccgc gcccgtcgcc tacgctttcg tcatcgagca
     5041 tgaaggcgcc gcagcggcgc ttgatctttc cgtcgcgctc gccgccctgg ctctcgccgc
     5101 cgccgcaatt ctggccgtcc gcttcggcag cctgaagcgc cgcgccgatt gacccgcccg
     5161 ccggtggacg ccgatcggtc cgaagcgcta ggttcaatga aacggctgag cgaggaggtc
     5221 ggatcggccc atgtcgagag agcgccgaaa gaggatcgcc tttctggctt tcgtcatcgc
     5281 cggggcatgg gcatgggcgg cgccgggcct cgccgaaccg cggctgatca gcctcgacga
     5341 cttgccgcca gaccagcaga aggtcgtctg ggagcgcgtc gaccgcttcg ccgaatatgc
     5401 ggtgatcctg aaggattgcg tggccgattc tcactttgag acccgcttcg tcgaggcggt
     5461 caaagcttgc gtcgagccga acacggtcag gcgcgtggtc ggctattatc agcagcgcgc
     5521 cgcgagcctt gaccgacgct tcagccggac cttttgctcc gagaagaaat tcacccagaa
     5581 caatgtcgcg caaaagctga agtccaccct cgataacctg gtcgattacg gccacaatct
     5641 ctgcatcgcc tatttgaaga ccggcgcagg cctgcgatag gaacgaggcg cgtcccttct
     5701 ccccgcttgt acccgtttag cgctctagag ctaggcgcgc tgtggcgtga gggcaagcct
     5761 tgcgccgagg aaatcggcga ggggacggag ccctagctcg acgccgattc accccttcac
     5821 gacgccatcc gcaggcggcc cttcgcgtag gaccgcagcg ccgaatattt atccgaatta
     5881 actccgtgag cggagtgctg cgaacagaag gtttaagcga gcgtgttttg atgagcgccg
     5941 tttcctcctt cgggttcgcc tgttcccccg cagccgttac ccgcaactgc cgccgcaatc
     6001 tccgcgagag ttgcggcggc ttttttgcgc tcagcccgca aacgacaggc ctttgatctt
     6061 ttgcttggcg agatctacga ccaactctga cggctatacc aattgtcgat atctcttttc
     6121 acttgatctt tagcgatccc atatcgctcc tgaatcttgc cttcgagctg gtcgcggctg
     6181 ccgttaatga ccgatagatc gtcgtcggtg agtttccccc attgctcctt gaccttgcct
     6241 ttcagctgct tccaattccc ctcgactctg ttccaatcca tagctgacct ccgtttttgc
     6301 agacggacaa acgtctggag agaaagcagg ttcccggacc gccgccagga aacggaacta
     6361 ttccatcatc ccggcgtttg tagttgcttc tctcgcgaag gaatacctcc gtgggcacga
     6421 ttctgatcat catcctgata ttgattctat tgggcggcgg cggcgggtac tacgcgcatg
     6481 gccgctatgg cggcgccggg ctcggcggcg tgctcgggtt cgtcctcatc attctgatta
     6541 tactttggct catcggagcg ttcagcgccg gcgtacatac cccgccttag aagtctgagc
     6601 ggatgccgcg tatcaagaaa gtatcagttc agaaggtgtc catccatacc gcttaaccaa
     6661 tcgagccagg agcaaggcgg ccggcgacac tataatgacg acaagaaact tctcgtggtc
     6721 cccttcgcgg cggcgcgtcc ctcgcaaaat tatcgcgatc ttagcatcgc cgagcccgcg
     6781 ccctcggtgg agatcagacc cctcgtttcg caaatcgaga cgaatcgttg cagccgcttg
     6841 atccgaaagc gtggcgcgac aaagccgcgg aggcgcgcgc cctcgccgaa acgatgcaca
     6901 atcaacgtgc gatcgaggcc atgctcaaag ctgcgctgga gtacgagcgc cttgctgcgg
     6961 tagccgaggg gctgcttcgc aatgtggcga gagggcgaat tccataaaac cggccaaggc
     7021 cggagcggct cgccaagtca tccccttctg agagaacaag gcgcgcgttc cctccatcac
     7081 ggattgaact gcggatttcc cttgtcgtgc atcagcacaa tgcccttgtc gatgtaagtg
     7141 attgcggccg cggtcttctc ttggccatcg cgcgcgctca cgttcgagat gatgccggcg
     7201 caatctttcg ccacgccgtt tcgaatttgc tcgatgccga agacgt
//
LOCUS       DATGJW010000334         7403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_474737, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000334 DATGJW010000000
VERSION     DATGJW010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7403
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_474737"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(223..990)
                     /locus_tag="VKU03_10275"
     CDS             complement(223..990)
                     /locus_tag="VKU03_10275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71695.1"
                     /translation="MMDETDEARLVALLDNQLDEEARRAIEARLAAEPELRARFQTLA
                     AGGLPFAQAFESALAEAPVARMRAKLDAPGGAMARSSPRRRWAGAAAAAIVLFGAGWA
                     GGRYVSPPRPLAPMASADRDTWRDAVASYVALYTRETFAGGARDEAAALARLSQALGV
                     ALTPDTVALPDLTFKWADMLAYEGAPLGQIAYLDSGSPVVFCIIRNRQKDAPIKTETR
                     EGLAIASWAHDGRGFLVGGRIAPDRATQLAGMLASRF"
     gene            complement(987..1535)
                     /locus_tag="VKU03_10280"
     CDS             complement(987..1535)
                     /locus_tag="VKU03_10280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007633171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HLJ71696.1"
                     /translation="MARRRGVSMASRDEIRAGLTENLARLWRYGLALSSARDTAEDLV
                     QATALRALERAEQFQSGTRLDRWLFAILRSIWLNEIRARRIRQGQGFVDPDQVLFSDG
                     AEIETNIFTGQVLNAVHRLPEAQRETVLLVYVEGLSYAEAAEFLGTPIGTIMSRLAAA
                     RVALAKLRSPDSATASARGSAK"
     gene            1610..2224
                     /locus_tag="VKU03_10285"
     CDS             1610..2224
                     /locus_tag="VKU03_10285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018898509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ71697.1"
                     /translation="MSFTMMRVGLPRSAAYLAFALTAIFAAPALASGGGGGGGGGGGE
                     SSSSSSHQDHSKSQTDLTTCERGKVWNAKTHKCLPRHSGVLPDSELADYAYALAKAER
                     YEEALQVLDLSQNPNTPKALNYRGYITRKLGRIDEGIGYYLKSVALDPNYAQVREYLG
                     EAYVIQGKFDLAKEQLGKIEAICGSTDCEEYEDLQKALTDAHNL"
     gene            2251..2679
                     /locus_tag="VKU03_10290"
     CDS             2251..2679
                     /locus_tag="VKU03_10290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850290.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLJ71698.1"
                     /translation="MKTVLRAIESIGRAGNLLALVGRVLSSALFINEGVSQIGDVAGT
                     MDYMRGFGVWPQLVPLVILAELGGGLLVLFGLATRVAAVGLAAFTLLAALFFHRDFSD
                     LDQYIHFQKNLAIAGGFLALAAYGGGAWSLDAWLKKRTGM"
     gene            2733..3344
                     /locus_tag="VKU03_10295"
     CDS             2733..3344
                     /locus_tag="VKU03_10295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HLJ71699.1"
                     /translation="MSVSSLLLFAGVYAVAVATPGPGVAAVVARVLARGLGGIMLFIA
                     GFVVGDLIWFAVAATGLAVLAHQFAALFTLIRCAGVLYLLWLAWRIWRAPAGAEEQTI
                     TPSDRGARLFFGALSLTLGNPKVIVFFLSIMPLVVDVHAMNAEAFTAIAVTMPLVLAP
                     VLAAYALAANRARALFRSPRAMKIVNRSAAGVMAGAAIAVATR"
     gene            complement(3463..4017)
                     /locus_tag="VKU03_10300"
     CDS             complement(3463..4017)
                     /locus_tag="VKU03_10300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093357.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HLJ71700.1"
                     /translation="MHSHDRHRCHAMREAYRAGRFGPEHRRHWGGGREGRVFDHGDLR
                     LVIMALLEEKPRHGYEIIKALEERVGEGYSPSPGVIYPTLTLLEEVGHATVAEEHGGR
                     KLYTLTEEGKAFLNANRTTAEAIFARFSDQGARRGPPPQVRRAVENLFAAVRIRLRSG
                     PLSDAQVRAIADAIDAAAKAVEQV"
     gene            4145..4399
                     /locus_tag="VKU03_10305"
     CDS             4145..4399
                     /locus_tag="VKU03_10305"
                     /inference="COORDINATES: protein motif:HMM:NF016268.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BrnT family toxin"
                     /protein_id="HLJ71701.1"
                     /translation="MEDGAFQWDDAKAAENYAKHGVTFETARAVFKDPFALDWRDERR
                     AYGEDRYVAIGISRRTPRLCRLHNALRRHPDYFGQRGRAL"
     gene            4423..4749
                     /locus_tag="VKU03_10310"
     CDS             4423..4749
                     /locus_tag="VKU03_10310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006698844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ71702.1"
                     /translation="MAKPTARDWGRFDAMNNEQRHSAALDDPDAQPLTPEDMARMRRT
                     PQARVIRRALGLSQEEFAARFRIPLGTLRDWEQGRKAPDAAAQAYLTVIGRNPDAVSE
                     ALLRGS"
     gene            complement(4759..5832)
                     /gene="hemH"
                     /locus_tag="VKU03_10315"
     CDS             complement(4759..5832)
                     /gene="hemH"
                     /locus_tag="VKU03_10315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004325 - ferrochelatase activity
                     [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrochelatase"
                     /protein_id="HLJ71703.1"
                     /translation="MNAPTPSEAFRAADARPDGHPAAKHGRIGVLLVNLGTPEATDYW
                     SMRRYLKEFLSDRRVIETPRLLWWPLLNLVILSRRPRARGRDYETIWNKARNESPLKT
                     ITRASAEKLAAAIAESGRDPAGRCLVDWAMRYGEPALGKRLRALQAAGCDRILLVPLY
                     PQYCAATTATVCDKAFETLKTMRWQPTLRVAAPYYDDPVYIGALAQSVRRSLDALDFE
                     PEVLLVSFHGIPRAYFDKGDPYYCHCAKTWRLLREALRMSEQRMPLAFQSRFGAAEFL
                     QPYTDETIKALAKSGVKRLAVVTPGFAGDCLETLEEIGVENAGYFRAGGGEKYAALPC
                     LNDSDEGMRVIEAVALRELQGWL"
     gene            complement(5854..6255)
                     /gene="msrB"
                     /locus_tag="VKU03_10320"
     CDS             complement(5854..6255)
                     /gene="msrB"
                     /locus_tag="VKU03_10320"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613277.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLJ71704.1"
                     /translation="MTVAEFAVTRSEDEWRRLLTPEQYHIMREHGTERAGSCALNYEK
                     RPGAFTCAGCGQTLFVSKKKFESGTGWPSFNDPEPGAVETSTDRSWGMARTEVHCSRC
                     GSHLGHVFEDGPPPTGLRYCINGVAMNFTPE"
     gene            complement(6368..>7403)
                     /locus_tag="VKU03_10325"
     CDS             complement(6368..>7403)
                     /locus_tag="VKU03_10325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ71705.1"
                     /translation="SWWAAASIATRACATLTLFAIFIVGIWLNIHGLASIGEIIAFMS
                     LAAMLIARLEQIVGFANYLVLQAPRLAQFFDVLDTASTVADRPGGVAVGRLQGEVTFE
                     SVSYSYDGLRDALNGLSFHVPPGRTIALVGVTGSGKSTTLSLLHRVFDPTKGRISIDG
                     RDLKDMTLTSLRRNIGVVFQEPLLFARSIEDNLRVGKPDASAEEIERALERAQAADFV
                     ARLPQGLSTLISERGRTLSGGERQRLAIARALLKDPPILILDEATSALDAKTERQFQE
                     ALKAATAGRTTFVIAHRLATIRDADEILVFDEGRIVERGGFDALVAAGGHFAALARAQ
                     FIDGEIAAAQ"
BASE COUNT         1214 a         2429 c         2456 g         1304 t
ORIGIN      
        1 cgcgccgacg ccgtcctggt tgacgacgat tccgccttcg agaatgagat cgaaacgcgc
       61 gggcatgaga cagcgcctcc tcgcgccgcg aaggcgcgct caaatcgtac gtcgtcattg
      121 cgagcgaagt gaagcaatcc atgcggcgcc cgacgtcgct tggattgctt cgtcgcttcg
      181 ctcctcgcaa tgacggcgat caggggccgg ctgtcaatgc ccttaaaagc ggctcgccag
      241 catgccggcg agttgggtcg cgcggtccgg cgcgatgcgg ccgccgacga gaaagccgcg
      301 gccgtcatgc gcccaggagg cgatggcgag gccctcgcgc gtttcggtct tgatcggcgc
      361 gtctttttgg cggttgcgga tgatgcagaa gacgaccggg ctgccgctgt cgagataggc
      421 gatctggccc aagggcgcgc cctcataggc gagcatgtcg gcccatttga aggtcaggtc
      481 cggcagcgcg accgtatccg gcgtcagcgc gacgccgagc gcttgcgaca ggcgcgccaa
      541 ggccgccgct tcgtcgcgcg cgccgccggc gaaagtctct ctcgtgtaga gcgcgacata
      601 gctggcgacg gcgtcgcgcc acgtgtcgcg atccgccgaa gccatcggcg cgagcgggcg
      661 gggcggcgag acataacgcc cgcctgccca gccggcgccg aacaggacga tcgccgccgc
      721 agccgcgccg gcccagcgcc gccgcggcga agaccgcgcc atggccccgc ccggggcgtc
      781 gagcttcgcc cgcatgcgcg cgaccggcgc ttcggcgagc gccgattcga aggcttgcgc
      841 gaaaggcagg ccgcccgccg ccaaagtctg aaagcgggcg cgcaattccg gctcggcggc
      901 gagccgcgcc tcgatggccc ggcgcgcctc ttcgtcgagt tgattgtcca gcaaggcgac
      961 gaggcgggcc tcgtccgtct cgtccatcat ttggcgctcc cccgcgctga cgcggtcgcg
     1021 ctgtcgggcg agcgcagctt ggcgagcgca actcgggcgg cggcgagccg gctcatgatc
     1081 gtgccgatcg gcgttcccag gaactccgcc gcttcggcat aggaaagtcc ttcgacatag
     1141 acgagcaaca ccgtttcgcg ctgcgcttcg ggcaggcgat gcaccgcatt taacacttgg
     1201 ccggtgaaga tattcgtctc gatttcggcg ccgtcgctga acaggacctg atcgggatcg
     1261 acaaagcctt gcccttgacg aatccgccgg gcgcgaattt cgttcagcca gatcgagcgg
     1321 aggatggcga aaagccagcg gtcgagccgc gtgccggatt ggaattgctc tgctctttca
     1381 agggcgcgca aggcggtcgc ttgaacgaga tcctcggccg tatcgcgggc ggacgacagc
     1441 gccaagccgt aacgccatag ccgcgcgagg ttctcggtga ggcccgctct gatttcgtcg
     1501 cggctcgcca tgcttacgcc ccggcggcgc gccattgtca aaagcgcggc gcgaatagga
     1561 cgcgcagttg gcgtgtttcc gtcgcgcccg tcaatatgga gctcctccaa tgtcgttcac
     1621 aatgatgcgc gttggcctgc cccgctcagc cgcttatctc gcattcgcgc tgaccgcgat
     1681 cttcgccgct cccgctctcg cctcaggcgg cggcggggga ggagggggag gcggcggcga
     1741 aagcagcagc tcttcgagtc accaggatca ttcgaaaagc cagaccgatt tgacgacatg
     1801 cgagcgcggc aaggtatgga acgccaagac gcataaatgc ctgccgcgcc atagcggcgt
     1861 tctgcccgac agcgagctcg ccgactacgc ctacgcgctc gccaaagccg agcgctatga
     1921 agaggcgctg caggtcctcg atctgtcgca gaatcccaac acgccgaaag cgctcaacta
     1981 tcgcggctac atcacgcgca aactgggccg gatcgacgag ggcatcggct actacctgaa
     2041 atccgtcgcg ctcgatccga actatgcgca agtgcgcgaa tatctcggcg aggcgtacgt
     2101 catccaaggc aagttcgatc tcgccaagga gcagctcggc aagatcgaag cgatttgcgg
     2161 ctcgaccgat tgcgaggaat acgaagactt gcaaaaggcg ctgaccgacg cccataatct
     2221 ttgatcttta acagcgcgcg gctgcaaggc ttgaaaacgg tcctaagggc gatcgagtcg
     2281 atcggccgcg ccggcaacct gctggcgctt gtcggccgcg tgctgtcatc ggcgttgttc
     2341 atcaatgaag gcgtctccca gatcggagac gtcgccggca cgatggacta tatgcgcggc
     2401 ttcggcgtct ggccgcagct cgttcccttg gtgattctcg ccgagctcgg cggcggcctg
     2461 ctcgtgcttt tcggcttggc gacgcgcgtt gcggcggtcg gtctcgccgc ctttacgctc
     2521 ctcgccgcct tgttcttcca ccgggatttt tccgatctcg atcaatacat ccatttccaa
     2581 aagaacctcg cgatcgccgg cggctttctc gccttggcgg cgtatggcgg cggcgcatgg
     2641 tcgctcgacg cctggctgaa aaagcggacc ggaatgtgag gcttggaaca ggcgcgcttt
     2701 tgcctttaac tggcgtgaga ggagcctgat cgatgtccgt ctcgtctctg cttttgttcg
     2761 ccggcgtcta tgcggtcgcc gtggcgacgc cgggcccggg cgtcgccgcg gtcgtcgcgc
     2821 gcgtcctggc gcgcggcctt ggcggaatca tgctctttat cgccggcttt gtcgtcggcg
     2881 acctcatttg gttcgccgtc gcggcgaccg gcctcgccgt gctggcgcat caattcgcgg
     2941 cgctcttcac gctgatccgc tgcgccggcg tcctctatct gctttggctc gcctggcgca
     3001 tctggcgcgc gccggccggc gcggaggagc agacgatcac gccgagcgac aggggcgcgc
     3061 gccttttctt cggcgctttg tcgctgacgc tcggcaatcc caaagtgatc gttttctttc
     3121 tgtcgatcat gcccctggtg gtcgacgtcc acgcgatgaa cgccgaggct ttcacggcga
     3181 tcgccgtcac gatgccgctc gtcctcgctc cggtccttgc cgcttatgcg ctcgccgcca
     3241 accgcgcccg cgccttgttc cgctcgccgc gcgcgatgaa gatcgtcaac cggagcgccg
     3301 ccggcgtgat ggccggcgcc gcgatcgcgg tcgcgacgcg ctaggcttct ggtttcgcgc
     3361 catgtcattc ccgcgcaaag cggaatccat gccggccggc cttcatggcg cggcggttcc
     3421 ctggtttcct gcctgcgcgg gaacgacaga ggcggcttgc gcctatacct gctcgaccgc
     3481 cttggcggcc gcgtcgatcg cgtcggcgat ggcgcggact tgcgcgtcgc tcagcgggcc
     3541 gctgcgcagc ctgatcctca ccgcggcgaa caggttctcg accgctctac gcacttgcgg
     3601 cggcgggccg cggcgcgcgc cctggtccga gaagcgcgcg aaaatcgcct cggctgtcgt
     3661 gcgattggcg ttcagaaagg cctttccctc ctcggtgagc gtgtagagct tccggccgcc
     3721 atgctcctcg gcgacggtgg cgtggccgac ctcttcgagc aacgtcagcg tcggatagat
     3781 cacgcccggg ctcgggctgt agccctcgcc gacgcgctct tcgagcgcct tgatgatttc
     3841 gtagccgtgg cgcggcttct cctcgagcag ggccatgatg acgagacgga ggtcgccgtg
     3901 gtcgaagacc cggccttcgc gtccgccgcc ccaatggcgg cggtgctcag gcccgaaccg
     3961 gcccgcccga taggcctcac gcatcgcgtg acagcggtgg cggtcgtgtg agtgcatgaa
     4021 aaacatagga tttcctcttg ttacgacata atgcgatatg tcttgatttg agatatatct
     4081 tgttatgtcg gaatgtcaag agcggcgttg accattgaac gagctacggc taagccgtat
     4141 attcatggag gatggcgcat tccaatggga cgacgcgaaa gccgcggaaa actacgccaa
     4201 gcacggcgtc actttcgaaa ctgcccgggc ggttttcaaa gatccgttcg ctcttgattg
     4261 gcgtgacgaa cgccgggcct atggcgagga tcggtatgtc gccatcggga ttagccggag
     4321 gacgcctcgt ctatgtcgcc tacacaatgc gttgcgacgt catccggatt atttcggcca
     4381 gaggggccga gccctttgaa cgtcgaaagt accatgaaga aaatggctaa accaacggcg
     4441 cgcgattggg gtcgtttcga cgcgatgaat aacgaacaac gccactccgc cgcgctcgac
     4501 gatcccgacg cccagcctct gaccccggag gacatggcgc gaatgcgccg cacgccccaa
     4561 gcgcgcgtga tccggcgcgc gctcgggctt tctcaggaag agttcgcggc gcgtttccgc
     4621 attcccctcg ggacattgcg ggattgggag caggggcgaa aggcgccaga cgcagcggcg
     4681 caagcttatc tgacggtcat cgggcgcaat ccggacgcgg tatcggaagc tttgctccgg
     4741 ggctcctgag cgccgccgct atagccagcc ctgcagctcg cgcaatgcca cggcctcgat
     4801 cacccgcatc ccctcgtcgc tgtcgttgag gcagggcagg gcggcgtatt tctcgccgcc
     4861 gccggcgcgg aaatagccgg cattctcgac gccgatctcc tccagcgtct ccaggcaatc
     4921 gccggcgaag ccgggcgtca ccacggcgag gcgcttcacg ccgcttttgg ccagcgcctt
     4981 gatcgtctcg tccgtatagg gctggaggaa ctcggcggcg ccgaaacgcg actggaacgc
     5041 caacggcatg cgctgctcgc tcatgcgcaa agcttcgcga agcagacgcc aggtcttggc
     5101 gcaatggcaa taatagggat cgcctttgtc gaaataggcg cgcggaatgc cgtggaagga
     5161 gacgaggaga acttccggct cgaaatccaa ggcgtcgagc gaccggcgga ccgactgcgc
     5221 cagcgcgccg atatagaccg gatcgtcata atacggcgcc gcgacgcgca aagtcggctg
     5281 ccagcgcatc gtcttcaacg tttcgaacgc cttgtcgcaa accgtcgcgg tggtcgccgc
     5341 gcaatattgc gggtaaagcg gaacaagcag aatgcggtcg cagcccgccg cttgcagcgc
     5401 gcgcagccgc ttgccgagcg ccggctcgcc atagcgcatc gcccagtcga cgaggcagcg
     5461 cccggccgga tcgcggccgc tctcggcgat cgccgcggcg agcttctccg cgctcgcgcg
     5521 cgtgatcgtc ttcagcggac tttcgttgcg cgccttgttc cagatcgtct catagtcgcg
     5581 gccgcgcgcc cgcggccgcc gcgacaggat caccagattg agcagcggcc accagaggag
     5641 gcgcggcgtc tcgatgacgc ggcgatcgga gaggaactct ttcaggtagc gccgcatcga
     5701 ccaatagtcg gtcgcctccg gcgtgccgag attgacgagg agcacgccga ttcggccatg
     5761 cttcgccgcc ggatggccgt ccggccgcgc gtcggcggcg cggaaggctt ccgacggcgt
     5821 tggcgcgttc atggccgctc tctcaacgtc aaatcattcc ggcgtgaaat tcatcgccac
     5881 gccgttgata cagtagcgca ggccggtcgg cggcgggccg tcctcgaaga catggccgag
     5941 atgcgagccg cagcgcgagc aatggacctc ggtgcgcgcc atcccccagg agcggtcggt
     6001 cgaagtctcg accgcgcccg gctcgggatc gttgaagctc ggccagccgg tgccgctctc
     6061 gaatttcttc ttcgagacga acagcgtctg gccgcagccg gcgcaggtaa aggcgccggg
     6121 acgcttttcg tagttcaagg cgcaactgcc ggcgcgctcg gtgccgtgct cgcgcatgat
     6181 gtgatattgc tccggcgtga gcaggcgccg ccattcgtcc tcgctccgcg tgactgcaaa
     6241 ttccgcgact gtcatgggct attctccttg gccgcattct aacaagctgc gccgggagag
     6301 ggaaacgccg acctcttcgc gcgggttgcg ccccggcggt cccttttcgg cggagccggc
     6361 cgtttcctta ctgcgcggcg gcgatctcgc cgtcgatgaa ttgcgcgcgg gcgagcgcgg
     6421 cgaaatggcc gcccgccgcg acgagggcgt cgaagccgcc gcgctcgacg attcgcccct
     6481 cgtcgaagac gaggatctca tcggcgtcgc ggatcgtcgc caggcgatgg gcgatgacga
     6541 acgtcgtgcg tccggccgtc gccgccttga gcgcctcctg gaattgccgc tcggtcttgg
     6601 cgtcgagcgc gctggtcgcc tcgtcgagga tgaggatcgg cgggtccttg agcagcgctc
     6661 tggcgatggc gagacgctgg cgctcgccgc ccgacagggt gcggccgcgt tcgctgatca
     6721 aggttgagag gccctgcggc aggcgggcga cgaagtcggc ggcctgcgcg cgctccaggg
     6781 cgcgctcgat ctcctcggcg ctcgcgtcgg gcttgccgac gcgcagattg tcctcgatcg
     6841 agcgcgcgaa aagcagcggc tcctggaaca cgacgccgat attgcgccgc agggaggtga
     6901 gggtcatgtc cttaagatcg cggccgtcga tcgaaatccg gcctttggtc ggatcgaaga
     6961 cgcgatgcag gaggctcagc gtcgtcgatt tgcccgagcc ggtgacgccg accagggcga
     7021 tggtgcggcc aggcgggaca tggaaggaaa ggccgttcag cgcgtcgcgc agcccgtcat
     7081 aggaatagga gacgctttcg aacgtcacct cgccttggag ccggccgacc gcgactccgc
     7141 cgggccgatc cgcgacggtc gaggccgtgt cgagcacgtc gaaaaactgc gcgagccgcg
     7201 gcgcctgcag caccaggtag ttggcgaagc cgacgatttg ctcaagccgc gcgatcagca
     7261 tggcggcgag actcatgaac gcgatgattt cgccgatcga cgccagccca tggatgttca
     7321 gccagatgcc gacgatgaag attgcaaaga gcgtcaaggt cgcgcaagcg cgcgtcgcga
     7381 tcgacgccgc cgcccaccag gag
//
LOCUS       DATGJW010000335         7348 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1308014, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000335 DATGJW010000000
VERSION     DATGJW010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7348)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7348)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7348
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1308014"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..762)
                     /locus_tag="VKU03_10330"
     CDS             complement(<1..762)
                     /locus_tag="VKU03_10330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003525366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLJ71706.1"
                     /translation="MKIAAIETIRVKEFENLLWLNVHTDDGLTGLGETFFLPGTVETY
                     VHEYLAPRALGRDPLAIDALARDLAGYLGFRSTGAEMRGNSAFDIALWDLFGKATGQP
                     LAQLLGGFSRPTIRTYNTCAGASYMRTAKGQNTSNWGLGAAKRYDDLDAFLNRADELA
                     EDLLSEGITAMKIWPFDIAAERNHGQYISPAEMKAALAPFEKIRRAVGDRIDVMVEFH
                     SLWQLLPAIEIARALAPFVTFWHEDPIKMDSLADLK"
     gene            complement(775..1503)
                     /locus_tag="VKU03_10335"
     CDS             complement(775..1503)
                     /locus_tag="VKU03_10335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020011124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FadR/GntR family transcriptional regulator"
                     /protein_id="HLJ71707.1"
                     /translation="MALADVLTPENPGQSGGLVDQVMTAIKQRMRAERLAQGDPLPSE
                     NALALEFGVSRAVVREAFRSLAALKLIDIGNGRRARVAAIDPAVLGLILDHAVHTNQI
                     TIQQIYDVRRTIEMRTVALAALRRSADEADAIKALADAMARDFGARDQVMAHDIAFHE
                     AIARASRNPLFALIVGSFHIVTRQTWGIGWTSRPNDAERMASVACHGAIARAIAEREP
                     RVASAAMADHFDLSVKWLLAAGVN"
     gene            1682..2689
                     /locus_tag="VKU03_10340"
     CDS             1682..2689
                     /locus_tag="VKU03_10340"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLJ71708.1"
                     /translation="MKHEPATIRDVAKAAGVSPATVSRYLNKSIALPAETAARIERAC
                     KRLAYRPNQLAKRLTLGSSELIGLVTPEIANPFFATLAAAAEDEARRLGYSLLIMSTG
                     GDPQMEFAHIDRIDSRHVDGLVILTNRPDDGRLRDIITGRKDVVLVDEDVPGAEIGRI
                     FVENEAGAYNATRHLLDAGHRKIAHIGGPKDLFSTRERFNGFARALADAAIPMDDSLV
                     RFGAYERSAGYLATRDILAGGAPTALFAGSDYLAIGALTALREMGIEAPRDFSLIGFD
                     DMPFAELLHPPLTTVRQPIEEMGREGVRTLLRQIKDNAAPGLARLKTELVARGSVAPP
                     G"
     gene            2724..3734
                     /locus_tag="VKU03_10345"
     CDS             2724..3734
                     /locus_tag="VKU03_10345"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLJ71709.1"
                     /translation="MRVTKTAIAALALAVGLGAPSLVHSAEKFSAVYVMSDNLGDKGF
                     NDSAAAGFRRAEKEGVKIKFLQASPSDPQLWRQNLEATSDSGAWSVIFTGPGMHDNLA
                     AVAPKHPNQKYVYFDDELKLPNVLSVKYAQNEGSYLAGALAGVVATDKADFPLSTASK
                     KVGLVAGMDLPVIQDFIVGFKQGAKTIDPSIDIQVSFIGNFNDAQKAYELTRNMLNGG
                     VDVVFNVAGPAGLGILKAAADANKYAIGVDSDQNGLYPKNVIASMLKQIGNSIYDSIK
                     QIEAGKAPFGSLEIYGLKNDGVGLVYNDALTPAAVKAKVDEAKAKVLSGAIKVDSAFK
                     TE"
     gene            3794..5341
                     /locus_tag="VKU03_10350"
     CDS             3794..5341
                     /locus_tag="VKU03_10350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71710.1"
                     /translation="MSEGSAAPLLQLRGVVKKFDDKVALDRVDLIVERGEVHVICGEN
                     GAGKSTLMNILAGILKPDSGEIILDGVPVGFADPLAASRAGIGMVHQHFKLIPSMTVA
                     ENLYLNRQPRRWGFLADRDAMRKDAAALIARYHFDLDPDARIARLSVGQRQRVEILKA
                     LGFDARILILDEPTAVLTPPEVEELLGVIAGLKARGRTILFITHKLREVKAASDRVTV
                     LRHGRRISTRPTAGLSEAAIARDMVGRDMRPPKRGEALRDQRRGAAVLQVSGLAMTTA
                     AGRRLLDRVDIEIHPGEIVGIAGVDGNGQTELSEAIAGLVPVQAGSIRLAGADMTRAS
                     PQRRRALGLSFIPEDRLDRGLSATMTIAENAAASNYRLKRLIGPAGLIRSAARDAFAL
                     EKIGQYDIRGAKPALPVGQLSGGNMQKVVIAREMEKEPAALIVAQPTRGLDVGAAEFV
                     HAQILAAADRGCAVLLISSELAEIFALADRIAVMFRGRLLKVLDRAAASEETVGLLMN
                     GAELEAA"
     gene            5341..6369
                     /locus_tag="VKU03_10355"
     CDS             5341..6369
                     /locus_tag="VKU03_10355"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71711.1"
                     /translation="MTGETKRLARAIAGPVAIVAAVLAGGFVLVLIISSDPVTAYRDL
                     LFANFYSRSNFALFVNRTMPLLLIGLGVVFSFRAGVFNVGGEGQLYFGAMAAAAAALA
                     LPSAPFPLGALASIVAAIAAGALWGWIPGVLKVRLDVNEVVSTLMLNFIALLTTEYLV
                     TNPLRDQTAYGAVSFVIPRGAWIPEIPGLPGATSGAVVAVLIAPLAWLALFRTEWGAN
                     LRAAGSNMRFAEAVGVDAGKQIIIAMLISGGLSGLAGAFYVLGIGHRFEQNFSPAFGL
                     IGLTVALLARLHPIGVLATATFYALVLNGAAYMQIDTDVPRSIVGLLTGFLVLFMTAR
                     PRRGLIAV"
     gene            6392..7291
                     /locus_tag="VKU03_10360"
     CDS             6392..7291
                     /locus_tag="VKU03_10360"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71712.1"
                     /translation="MASVLDQTTPILLAALAAMITTRANILNVAVEGMMLVAAFVAIA
                     VGEATNDVALAFSCAVLAAILMSLLFGFVAIVFQADFIVAGLGLNILAAGGTLFVLEE
                     FYQDPGGLRPDHFPDIWHVPSGSLDWAPVIGPAIERQSFIVLLALALAPLSALLLYRT
                     PLGYSLRAVGEDEQAARAVGIHVVRMKLFAVAASGLLAGLAGAQLAMDRLHFFLPDMT
                     AGRGFIGLAATLFGGGLPWATTGAAFLFGFFGALGDRLQLLQAPSQLVLMAPYLAATL
                     GLIVARWRAAARNRPAPVASAET"
BASE COUNT         1233 a         2340 c         2353 g         1422 t
ORIGIN      
        1 cttcagatcg gcgagactgt ccatcttgat cgggtcttcg tgccagaacg tcacgaaggg
       61 ggcgagcgcc cgcgcgatct cgatcgccgg cagcaattgc caaagcgaat ggaactcgac
      121 catcacgtcg atccggtcgc ccaccgcgcg gcgtatcttc tcgaaaggcg cgagcgcggc
      181 tttcatctcg gcggggctga tatactggcc gtgattcctc tccgcggcga tgtcgaaggg
      241 ccagattttc atcgccgtga tcccttccga caggagatcc tcggcgagct cgtcggcgcg
      301 attgagaaag gcgtcgagat cgtcgtagcg cttggccgcg cccaatcccc aattcgacgt
      361 attctggccc ttggcggtgc gcatatagct cgcgccggcg caggtattgt acgtacggat
      421 cgtaggccgg ctgaagccgc cgagcaattg cgccagaggc tggccggtcg ctttgccgaa
      481 taaatcccag agggcgatgt cgaaggccga attgccgcgc atctcggcgc cggtcgagcg
      541 aaagccgaga tagccggcga ggtcgcgggc caaggcgtcg atcgccagcg ggtcacggcc
      601 gagcgcgcgc ggcgccagat attcatgaac ataagtctcg accgttcccg gcaagaagaa
      661 ggtctcgccg aggccggtca agccgtcgtc ggtatggacg ttgagccaaa gaagattctc
      721 gaactccttc acccggatcg tctcgatggc ggcgatcttc atcatcgtcg tccttcaatt
      781 cacgccggcg gcgagaagcc atttgaccga gagatcgaaa tgatcggcca tcgccgcgct
      841 ggcgacgcgc ggctcgcgct cggcgatcgc gcgagcgatc gcgccgtggc aggcgacgct
      901 cgccatgcgc tcagcgtcgt tcggccggct cgtccagccg atcccccaag tctggcgcgt
      961 gacgatatgg aacgatccga cgatcagcgc gaagagcgga ttgcgcgaag cgcgcgcaat
     1021 tgcctcgtga aaagcgatgt catgcgccat gacttggtcg cgcgctccaa aatcgcgcgc
     1081 catcgcgtcg gccagcgcct tgatcgcgtc cgcttcgtcg gcgctgcggc gcaaggccgc
     1141 cagcgccact gtgcgcatct cgatggttcg ccgcacgtcg taaatctgct ggatggtgat
     1201 ctgattggtg tgaacggcat ggtcgagaat gagtcccagc acggcgggat cgatcgccgc
     1261 gaccctggcg cgccgtccat tgccgatatc gatcaatttc agcgcggcga gcgagcggaa
     1321 cgcctcgcgc accacggcgc gggagacgcc gaattcgagc gcgagcgcgt tctcgctcgg
     1381 cagcggatcg ccttgcgcta ggcgctcggc gcgcatccgc tgcttgatcg ccgtcatgac
     1441 ctggtcgacc aagccgccgg actgaccggg gttttcaggg gtcagcacat ctgcaagcgc
     1501 catggcctat ccgacaggtg tggagcaacc tgtatgaaaa ttgacgcaag gctgcctgtc
     1561 aagcgcgttg gtgccggcgg ggcaggagct tcggccccgt cctcatgagc gggtccatcg
     1621 ttcctataga atcggtgata aaccggttta ctaaaccggt tttttagcct acaatgaaag
     1681 cgtgaagcac gaacccgcca cgatacgcga cgtcgccaag gcggccgggg tctctccagc
     1741 gacggtttcg cgttatctca acaaatcgat tgcgctgccg gccgagacgg ctgcgcgcat
     1801 cgagagggcc tgcaagcgcc tcgcttatcg gcccaatcag ttggcgaagc ggctgaccct
     1861 tggcagttcc gaactgatcg gccttgtcac gccggaaatc gccaatccgt tcttcgcgac
     1921 tctcgccgcg gccgcggaag atgaagccag gcgccttggc tattctttgc ttatcatgag
     1981 tacgggcggc gatccgcaga tggagttcgc gcatatcgac cggatcgatt cgcgccatgt
     2041 cgacgggctc gtcattctga ccaatcggcc tgacgacggc cgtttgcgcg acatcatcac
     2101 cggccgcaag gatgtcgtgc tggtcgatga ggatgtgccg ggcgcggaaa tcggccgcat
     2161 tttcgtggag aacgaagcgg gcgcctataa cgcgacccgg catcttctcg acgccgggca
     2221 tcgcaagatc gcgcatatcg gcggcccgaa ggatttgttc agcacgcgcg aacggttcaa
     2281 cggcttcgcc cgcgcgctcg ccgacgcggc aattccgatg gacgattctc ttgtacggtt
     2341 cggcgcgtac gaacgcagcg ccggctatct cgcgacaaga gacattctgg cgggcggcgc
     2401 gccgacggct ttgttcgccg gaagcgacta tctcgctatc ggcgctttga cggccttacg
     2461 cgaaatgggg atcgaggcgc cgcgcgattt ctcgcttatc ggcttcgacg acatgccctt
     2521 cgccgaattg ctgcatccgc cgctgaccac ggtgcgccag ccgatcgagg agatgggtcg
     2581 cgaaggggtg cggacgctgc tgcggcaaat caaggacaat gccgcgccgg gtttggcgcg
     2641 gctgaagacg gagctcgtgg cgcgcggttc ggtcgccccg cccggctaag ttcatggtgg
     2701 gagtgaaaga gggagatgat actatgcgtg tgacaaaaac ggcgatcgct gcgctcgcgc
     2761 tggctgtggg cctcggcgct ccaagcctcg tccattcggc cgaaaaattc agcgccgtct
     2821 atgtgatgag cgacaatctc ggcgataagg gcttcaacga ttcggcggcg gccggtttcc
     2881 ggcgagcgga aaaggagggc gtcaagatca agttcctgca agcttcgccc agcgatccgc
     2941 aattgtggcg ccaaaatctc gaggcgacat ccgacagcgg cgcctggagc gtcatcttca
     3001 ccggacccgg catgcacgac aatcttgccg cggtcgcgcc aaagcatccg aatcagaaat
     3061 atgtctattt cgacgacgag ctgaagctgc cgaacgtctt gtcggtgaaa tacgcccaga
     3121 acgaagggtc ctatctcgcc ggcgccttgg ctggcgtggt ggcgaccgac aaagcggact
     3181 ttccgctgtc gacggcgtcc aagaaagtcg gcctcgtcgc gggcatggac ttgccggtga
     3241 ttcaagattt catcgtcggc ttcaagcagg gcgccaaaac aatcgatccg tcgatcgata
     3301 tccaagtctc gttcatcggc aatttcaacg acgcgcagaa agcctacgag ctgacccgca
     3361 acatgctcaa tggcggcgtc gatgtcgtct tcaacgtcgc cggaccggcc gggctcggca
     3421 ttctgaaggc ggcggccgac gccaacaaat acgcaatcgg cgtcgattcc gaccagaacg
     3481 ggctttaccc gaaaaacgtc atcgcctcga tgctcaagca gatcggcaat tcgatctatg
     3541 attcgatcaa gcagatcgaa gccggtaagg ccccgtttgg aagccttgaa atctacggac
     3601 tgaagaatga cggcgtcggg ctggtctata acgacgcgct gacgccggcc gcggtgaagg
     3661 ccaaagtgga cgaggccaag gccaaggtgc tgagcggcgc gatcaaggtc gacagcgctt
     3721 tcaagacgga gtgaggcgcc ggggcggcgc gccccgcgcg ccgcccttga tcttccgcga
     3781 aggagccgaa gccatgagcg aagggtcggc agcgcctttg ctgcaattgc gcggcgtcgt
     3841 caagaaattc gacgacaagg tcgcgctcga tcgcgtcgat ctcatcgtcg agcgcggcga
     3901 ggtccatgtg atctgcggcg agaatggcgc cggcaaaagc actctgatga acattctcgc
     3961 cggcatcctg aagccggatt ccggcgagat cattctggat ggcgtcccgg tcggcttcgc
     4021 cgatccgttg gcggcgagcc gcgcaggcat cggcatggtc catcagcact tcaagctcat
     4081 cccatcgatg acggtcgccg agaatcttta tctcaatcgt cagccgcgcc gttggggttt
     4141 cctcgcggat cgcgacgcca tgcggaaaga cgcggcggcc ttgatcgcgc gctaccattt
     4201 cgatctcgat cccgacgcgc gcattgcgcg cctctcggtc ggccagcgcc agcgcgtgga
     4261 gatcctcaag gcgcttggct tcgacgcgcg gatcctcatc ttggacgagc caaccgcggt
     4321 tttgacgccg cccgaagtgg aggagctgct cggcgtgatc gccggcctga aagcgcgcgg
     4381 acgaaccatt ctgtttatca ctcacaagct ccgagaagtg aaagcggcgt ccgaccgggt
     4441 cacggtgctt cgccatggcc gccgcatctc gacgcggccg actgccgggc tttccgaagc
     4501 ggcgattgcg cgcgacatgg tcgggcggga catgcgcccg ccgaaacgag gagaggcgct
     4561 gcgcgatcag cggagaggcg ccgcggtcct gcaagtttcc gggctcgcta tgaccacggc
     4621 ggccgggcgc cgcttgctcg atcgcgtcga tatcgagatc catccgggag agattgtcgg
     4681 catcgccggc gtcgacggca acgggcagac cgagctcagc gaggcgatcg ccgggcttgt
     4741 cccggtccaa gccggatcga ttcggctcgc tggcgccgat atgaccaggg ccagcccgca
     4801 gcggcggcgc gctctcggct tgagcttcat cccggaagat aggctcgatc gcggcctcag
     4861 cgccacgatg acgatcgcgg aaaacgccgc ggcgagcaat tatcggctca agcgactcat
     4921 cggccccgcc gggctgattc gctcggccgc gcgcgacgcc ttcgcgctcg agaaaattgg
     4981 ccagtacgac attcgcggcg ccaagcccgc tttgccggtc ggccagcttt ccggcggcaa
     5041 catgcagaaa gtggtgattg cgcgagagat ggagaaggag ccggcggcgc tcatcgtcgc
     5101 gcagccgacg cgcggccttg atgtcggcgc cgccgaattc gtccatgcgc agatcctcgc
     5161 cgccgccgat cgcggctgcg cggttcttct gatctcatcc gagctcgcgg agattttcgc
     5221 gctggcggac cgcatcgcgg tgatgtttcg cggccgcctg ctcaaagttc tggatcgagc
     5281 cgccgccagc gaggagacgg tcggtctgct catgaacggc gcggaactgg aagcggcgtg
     5341 atgacaggcg agacgaagag gcttgcgcgg gcgatagccg gccccgtcgc catcgtcgcg
     5401 gcggttttgg ccggcggctt cgtgctcgtt ctcatcatca gcagcgatcc ggtgaccgcc
     5461 tatcgcgacc ttctcttcgc gaatttctat tcgcgctcga atttcgcgct gttcgtcaac
     5521 cgaaccatgc cgcttctgtt gatcggtctc ggcgtggtgt tctcgttccg cgccggcgta
     5581 ttcaatgtcg gcggggaggg ccagctctat ttcggcgcca tggcggccgc tgcagcggct
     5641 ctggccttgc cctcggcgcc gtttccctta ggcgctttgg cctcgatcgt cgccgcgatc
     5701 gccgccggcg ctctgtgggg ctggattcca ggcgtcttga aggttcgcct cgacgtcaac
     5761 gaggtggtca gcaccctgat gctgaacttc atcgcgctgc ttacgaccga atatctcgtc
     5821 accaatcctt tgcgcgatca gaccgcctat ggcgccgtca gcttcgtcat cccgcgcggc
     5881 gcgtggattc cggagattcc cggcctgccg ggggccacga gcggcgccgt tgtcgctgtc
     5941 ctgatcgcgc cgctcgcctg gctcgccttg tttcgcacag aatggggcgc gaacttgcgg
     6001 gcggctggct ccaacatgcg cttcgccgag gcggtgggcg tcgacgccgg caaacagatc
     6061 atcattgcta tgctgatatc cggcgggctc tccggactgg cgggcgcgtt ctatgtgctc
     6121 gggatcggcc atcgcttcga gcaaaacttt tcgccggctt tcggcttgat cggcttgacc
     6181 gtcgctttgc tcgcgcggct ccacccgatc ggcgttctgg cgaccgcgac gttctacgca
     6241 ctggtcttga acggcgccgc ctatatgcag atcgacaccg atgttccgcg ctccatcgtt
     6301 gggcttctga ctggcttcct cgtccttttc atgaccgctc ggccgcgccg cggcctgatc
     6361 gcagtgtgac gccatgcagg cttctcagtt cttggcgtcg gtactcgacc agacgacccc
     6421 gattcttctg gcggctttgg cggcgatgat cacgacgcgc gccaacattt tgaacgtcgc
     6481 cgtcgaaggg atgatgctgg tcgcggcgtt cgtcgccatc gccgtcggcg aggcgacgaa
     6541 cgatgtcgct ctggctttta gctgcgcggt cctcgccgcg attttgatgt cgctcttgtt
     6601 cggcttcgtc gcgatcgtct ttcaggccga ttttatcgtt gccgggcttg ggctcaacat
     6661 tctcgccgcc ggcggcacgc tcttcgtgct ggaggagttc taccaggatc ccggcggtct
     6721 gcgcccggac catttccccg acatctggca cgtaccttcc ggctcgctcg actgggcgcc
     6781 ggttatcggc ccggccatcg agcgccaaag ctttatcgtg cttcttgctc tcgctcttgc
     6841 gcccttgtcg gctcttctgc tctatcgaac tccgctcggc tattccctgc gggccgtcgg
     6901 cgaagacgaa caggcggcgc gcgccgtcgg cattcatgtc gtccggatga agctcttcgc
     6961 cgtcgcggcg agcggcttgc tcgccggcct cgccggcgcg caattggcga tggatcgcct
     7021 ccatttcttt ttgccggaca tgacagccgg ccgcggcttt atcggcctcg ccgcgacgct
     7081 gttcggcggc ggcttgccct gggcgacgac cggcgccgcc ttcctgttcg gcttcttcgg
     7141 cgcgctcggc gaccggctgc aactcctgca agcgccgtcg caactcgtct tgatggcgcc
     7201 ttacctggcg gcgacgctgg gccttatcgt cgcgcgctgg cgggccgcgg cgcgcaatcg
     7261 cccggcgccg gtcgcgagcg ctgaaacatg atcgttccgc tggttcacga ttgcgatccg
     7321 ggcaatgacg acgcgcttgc gattctcg
//
LOCUS       DATGJW010000336         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1512713, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000336 DATGJW010000000
VERSION     DATGJW010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1512713"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1955)
                     /locus_tag="VKU03_10365"
     CDS             complement(<1..1955)
                     /locus_tag="VKU03_10365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HLJ71713.1"
                     /translation="MTEAAAVATTCPYCGVGCGVLAETGNEGTLSLRGDPDHPANFGR
                     LCSKGSALIETIGLEERLLHPEIRGKRASWDDALDVVAATFSRTIADHGPDSVAFYVS
                     GQLLTEDYYVANKLMKGFIGSANIDTNSRLCMASAVAGHRRAFGSDTVPGTYEDLELA
                     DLVVLAGSNLAWCHPVLYQRIAAAKAQRPEMRIVLIDPRRTMTADIADLHLAIRADGD
                     VALFAGLLAFLAERGAVAEDYVAAFTTGFPQALAAARASSLDEITTATGLPLDQVVAF
                     YELFAQTEKTVTVYSQGVNQSSSGTDKVNAIVNCHLASGRIGRPGMGPFSVTGQPNAM
                     GGREVGGLANMLAAHMEIENAEHRDRVQRFWNSPTIAARPGLKAVDMFRAVAKGRIKA
                     IWIMATNPAVSMPEAGAVEDALRSCPFVAVSDASAKTDTLRLAHIALPAAAWGEKEGT
                     VTNSERRISRQRAFLPLPGEARPDWRIICDVAARMGFGEAFAYASPAAIFAEHAALSA
                     FENDGARAFDIGALAGLDPPQYAALAPTQWPAPDRGAPTGGRIFGGGRYFTPDHKARF
                     VAVSPKAEARTSLNFPFILNTGRVRDHWHTMTRTGKSARLSQHLAEPYIEIHPADAAR
                     FGIAGADIVRVSAAGEAILARALLSDR"
     gene            complement(1952..2281)
                     /gene="nirD"
                     /locus_tag="VKU03_10370"
     CDS             complement(1952..2281)
                     /gene="nirD"
                     /locus_tag="VKU03_10370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006205929.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008942 - nitrite reductase [NAD(P)H]
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrite reductase small subunit NirD"
                     /protein_id="HLJ71714.1"
                     /translation="MSWIAIGSLADIPLRGARCIATPAGRVAVFRTAADEVFALEDRC
                     PHKGGPLSQGMVHGDAVACPLHNWVIALRTGKALGADEGAVRTIPLKREGDSLFLASE
                     ALADRAA"
     gene            complement(2286..>3848)
                     /locus_tag="VKU03_10375"
     CDS             complement(2286..>3848)
                     /locus_tag="VKU03_10375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011425074.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLJ71715.1"
                     /translation="VAAAQLADESAAAFVHNDTPAKLKVTGIDLFSVGDFAEGEDRQE
                     IVLRDASAGVYKRVILKDNRIVGAVLYGEVADGAWFADLKKKQIDISPMRETLIFGQG
                     YQGSVPLDPMAAVAALPDDAEICGCNGVCKEKITSAIQSKSLSSLDDVRAHTKASASC
                     GSCTGLVEQLLKLTLGDAYQAVAIQPICSCTPLGHDDVRRLIKAKALKSISAVMHELE
                     WRTSCGCAKCRPALNYYLVCAWPGEYADDGQSRFINERVHANIQKDGTYSVVPRMWGG
                     VTSARELRAIADVVDRFHIPAVKVTGGQRIDMLGVKKEDLPAVWAELGKAGFVSGHAY
                     AKGLRTVKTCVGSEWCRFGTQDSTRLGIRIEKFMWGAWTPAKVKMAVSGCPRNCAEAT
                     CKDVGIICVDSGYEIHFAGAAGLDVKQTEILCSVKNETEAVEHIAALVQMYREQGRYL
                     ERIYKWAKRIGLAEIRRQIVESAEKRRAYFERFVFSQKFAQADPWAERASGKDKHEFR
                     PMATIGYLEAAE"
BASE COUNT          682 a         1247 c         1236 g          683 t
ORIGIN      
        1 tgccggtccg acagcaaggc gcgcgccagg atcgcctcgc ccgccgccga gacgcgaaca
       61 atatcggccc cggcaatgcc gaagcgcgcg gcgtccgcag gatgaatttc gatatacggt
      121 tcggcgagat gttgcgatag gcgcgcgctt tttccggttc gcgtcatcgt atgccaatga
      181 tcgcgcacgc gcccggtatt caggatgaaa ggaaaattca agctggtgcg cgcctccgct
      241 ttgggactga ccgcgacgaa gcgggctttg tggtctggcg tgaagtatcg gccgccgccg
      301 aaaatgcgtc cgcccgtcgg cgcgcctcga tccggcgccg gccattgcgt cggcgccagc
      361 gcggcatatt gcggcggatc gaggccggcg agcgcgccga tgtcgaaagc gcgcgcgccg
      421 tcattctcga aggcggagag cgcggcgtgc tcggcgaaaa tcgccgccgg cgaggcgtag
      481 gcgaaagcct ctccgaatcc catccgcgcc gcgacgtcgc agatgatccg ccagtcgggc
      541 ctcgcctcgc cgggaagcgg cagaaaggcc cgctggcgcg aaatccgccg ctcggaattg
      601 gtgaccgtcc cttctttttc gccccaggcc gcggcaggca aggcgatatg ggccaagcgc
      661 agcgtatcgg tcttggccga ggcgtccgag accgcgacga acgggcacga tctgagcgcg
      721 tcttcgacgg cgccggcctc gggcatcgaa acggccggat ttgtcgccat gatccagatc
      781 gccttgatcc gtcctttggc gacggcgcga aacatgtcga ccgccttcag gcccgggcgc
      841 gccgcgatgg tcggcgaatt ccagaagcgc tggacgcgat cgcgatgctc ggcattttcg
      901 atctccatat gcgcggcgag catattggcg agcccgccga cctcgcgccc gcccatggcg
      961 ttgggctgcc cggtgacgga gaaaggaccc atgccgggcc ggccgatgcg accgctcgcc
     1021 aaatggcaat tgacgatggc gttgaccttg tccgtccccg acgaagactg attgacgccc
     1081 tggctgtaga cggtgaccgt cttttccgtt tgcgcgaaga gttcatagaa ggcgacgact
     1141 tgatccagcg gcagtccggt cgccgtcgtg atctcgtcaa ggctcgacgc gcgcgccgcc
     1201 gccaatgctt gcggaaagcc agtcgtaaag gccgcgacgt aatcctccgc caccgcccca
     1261 cgctcggcga gaaaagcgag gaggcccgcg aacagcgcga catcgccgtc ggcccggatc
     1321 gccagatgaa gatcggcgat atcggcggtc atcgtgcggc gcgggtcgat cagcacgatc
     1381 cgcatttccg gccgttgggc cttggccgcg gcgatgcgct gataaaggac gggatggcac
     1441 caagcgaggt tcgagcccgc cagaacgacg aggtcggcga gttcgaggtc ttcgtaggtc
     1501 cccggtacag tatctgagcc gaaagcgcgt cggtgcccgg ccaccgccga ggccatgcag
     1561 aggcgcgaat tggtgtcgat attggccgag ccgataaagc ccttcatcag cttgttggcg
     1621 acgtagtagt cctcggtcag aagctggcca gagacgtaga aggcgacgga atccggccca
     1681 tgatcggcga tcgttcgcga gaacgtcgcc gcgacaacat cgagcgcgtc gtcccagctc
     1741 gcccgcttgc ctctgatctc gggatggagc agccgttcct cgagcccgat cgtctcgatc
     1801 aaggccgatc ccttcgagca gaggcggccg aaattggcgg gatgatccgg atcgccgcga
     1861 aggcttaacg tcccttcatt gccggtttcg gcgaggacgc cacagccgac gccgcaataa
     1921 gggcaggtcg tcgcaaccgc tgctgcttcc gtcacgccgc tcgatccgcc agcgcttcgg
     1981 atgcgaggaa gaggctgtcg ccttcgcgct tgaggggaat tgtgcgcacc gcgccctcat
     2041 ccgcgcccaa ggccttgccg gttcgaagcg caatgaccca gttgtgcagc ggacaggcga
     2101 ccgcgtcgcc atgcaccatg ccctggctta agggtccgcc tttgtgcggg caacgatctt
     2161 cgagcgcaaa aacctcgtcg gccgcggtgc gaaacaccgc gactctgccc gccggcgtcg
     2221 caatgcatcg cgcgccgcgc agcggtatgt cggcgagcga gccgatcgcg atccagctca
     2281 tccggctact ccgccgcttc caaatagccg atcgtcgcca ttggccggaa ttcatgcttg
     2341 tccttgccgg aggcgcgctc cgcccatgga tcggcctggg cgaatttctg gctgaagacg
     2401 aagcgctcga agtaagctcg gcgcttttcg gcgctttcga cgatttgtcg gcgaatctcg
     2461 gcgaggccga tccgcttcgc ccatttatag atgcgctcga ggtagcggcc ctgctcgcga
     2521 tacatctgga caagcgcggc gatatgctcg accgcttccg tctcattctt cacggagcag
     2581 agaatctcgg tctgcttgac atcgaggccc gcagcgccgg cgaaatggat ttcatagccg
     2641 gaatcgacgc agatgattcc gacatcctta caagtcgcct cggcgcaatt gcgcggacag
     2701 cccgagaccg ccattttcac cttggccggc gtccacgcgc cccacatgaa tttctcgatg
     2761 cgaatgccca ggcgtgtcga atcctgcgtg ccgaaccggc accattccga gccgacgcac
     2821 gtcttcaccg tgcgcaggcc cttggcgtag gcatggccgg agacgaagcc ggctttgccg
     2881 agctccgccc agaccgccgg caaatcctcc ttcttgacgc cgagcatgtc gatgcgctgg
     2941 ccgccggtga ctttgacggc gggaatatgg aagcggtcga cgacatcggc gatggcgcgc
     3001 aattcccgcg cgctggtcac gccgccccac atgcgcggca ccaccgaata cgtgccgtcc
     3061 ttctggatgt tggcgtgaac gcgctcgttg atgaagcgcg attgcccatc atcggcgtat
     3121 tcgcccggcc aggcgcagac cagataatag ttcagcgccg gccggcattt ggcgcagccg
     3181 catgacgtcc gccattcaag ctcgtgcatc acggcggaaa tgcttttcag cgccttggcc
     3241 ttgatcaggc gccgcacatc gtcgtggccg agcggcgtgc aggaacatat cggctggatc
     3301 gcaacggctt gataggcatc gccgagggtc agcttcaaca attgctcgac aaggccggtg
     3361 caggagccgc aggacgcgga agccttggta tgggcgcgga catcgtccag cgatgacagg
     3421 cttttggatt ggatggcgct cgtgatcttt tccttgcaaa cgccgttgca gccgcaaatc
     3481 tccgcgtcat ccggcaaggc tgcaacggcc gccatagggt cgagggggac gctcccctgg
     3541 tagccctggc cgaaaatcaa cgtctcgcgc atcggcgata tgtcgatctg cttctttttc
     3601 agatcggcga accaggcgcc gtcggctact tcgccataga gcacggcgcc gacaatccgg
     3661 ttgtccttga ggatgacgcg cttatagacg cccgccgacg cgtcgcgcag cacgatctcc
     3721 tggcgatcct cgccttccgc gaagtcgccg actgagaaga ggtcgatgcc ggtgactttg
     3781 agcttggccg gcgtgtcgtt atggacgaag gccgccgcgg actcgtcggc gagctgcgcc
     3841 gcggcgac
//
LOCUS       DATGJW010000337         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_894459, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000337 DATGJW010000000
VERSION     DATGJW010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_894459"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(273..2561)
                     /locus_tag="VKU03_10380"
     CDS             complement(273..2561)
                     /locus_tag="VKU03_10380"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ71716.1"
                     /translation="MPAPPPEKLRRAFAAYMSGDLGEAERLSRAVAAAKPDLFDAWHL
                     LGFVQAAMGRGDKALQSYDRALALQPNHAEAVVNRGAALHDLGRFEEALAAAERAVAL
                     NGAYPPAHGNRGLALHALRRYGEALAAFDRAIALKRDYGDAHAHRAATLFALDRREEA
                     LASYDRALAVNPRDAASHFNRGNVLRALGRLNDALAAYERALAIDAKHLGALANRGAA
                     LHEARRFAEALATFDALIALSPQDAEAHYNRGNSLHDLGRFAEAASSFDKALALKPDF
                     VEALYNRGNALFAQQRYAEALADFERTRAARPDHLYALERAANCVLQLCDWTKRGEYE
                     QALAADIRAGRSIVSPFTVLGFSGDPALQLQCARLYVAAAAPVVSSKPPLAPRGAKIR
                     IAYLSADFREHAIAYLIAELFELHDRAAFEIVGVSFGRDDGGPMRKRIAASFDRFIDA
                     RAMSDAAVVEALRALKTDIAVDLTGHTADSRLGVFARRAAPIQVSYIGFAATMGAPFI
                     DYVLLDSVVAPPGADAFFSEKIVRLPHSYYASDRKRPIAEKTPSREEAGLPPRGFVFC
                     CFNNVWKIAPATFDIWMRLLRAVDGAVLWLLPSNQAAEDNLKAEAARRGVDPARLVFA
                     PRMGLADHLARHRLADLFLDTLPYNAHTTASDALWAGLPVLTRPCEAFASRVAASQLQ
                     AIGLADLIAPDEAAYEALALNLAREPARLADVKRRLAENRLTAPLFDTPRLTRNIESA
                     FAAMIERETPSSPAKAGEGDRA"
     gene            complement(2707..>2958)
                     /locus_tag="VKU03_10385"
     CDS             complement(2707..>2958)
                     /locus_tag="VKU03_10385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLJ71717.1"
                     /translation="YAPDGRIDRIVALPCSQITCCAFGGKDLATLFVTSARGGLDAAK
                     LRAEPEAGAMFAVDVGTKGLADAPFMEARSPTAPLPPPL"
BASE COUNT          467 a          963 c         1117 g          411 t
ORIGIN      
        1 aggaatatcc ttccccgtga agcgaagcga aacgggggag ggggaccggc cgaaggccgg
       61 tggagggggc cgcgggcggg tgggattatc ctgaggggcc ggagccccct ccaccgcgct
      121 tacgcgcggt ccccctcccc cgctttcgcg ggggaggaat atcctccccc gtggagcgaa
      181 gcgtaacggg ggagggggac cggccgaagg ccggtggagg gggccgcggg cgggtgggat
      241 tatcctgagg gccggagccc cctccaccgc gcttacgcgc ggtccccctc ccccgctttc
      301 gcgggggagg agggggtttc gcgctcgatc atggcggcga aggcggactc gatattccgc
      361 gtcaggcgcg gcgtgtcgaa gagcggggcc gtcaggcgat tctcggcgag gcgccgcttg
      421 acgtcggcga gacgcgccgg ctcgcgcgcg agattcagcg ccaacgcttc gtacgccgct
      481 tcgtccggcg cgatcaggtc ggcaaggccg atcgcctgga gctggctcgc ggcgacgcgc
      541 gaggcgaagg cctcgcaagg ccgggtcagg accggcaggc cggcccagag cgcgtcgctc
      601 gccgtggtat gagcgttgta ggggagcgta tcgaggaaga ggtcggcgag gcggtggcgg
      661 gcgagatggt cggcgaggcc catgcgcggc gcgaagacga gccgcgccgg atcgacgccg
      721 cgccgcgccg cctcggcctt cagattgtcc tcggccgcct ggttgctcgg cagcagccac
      781 agaaccgcgc cgtcgacggc gcgcaacagg cgcatccaga tgtcgaacgt tgcgggcgcg
      841 atcttccaga cattgttgaa gcagcagaag acgaagccgc gcggcggcag cccggcttcc
      901 tcgcgcgacg gcgtcttctc ggcgatcggc cgcttgcggt cgctggcgta gtaggaatgc
      961 ggcaggcgga cgatcttttc ggaaaagaac gcgtcggcgc cgggaggcgc gacgacggaa
     1021 tcgagcagca catagtcgat gaacggcgcg cccatcgttg cggcgaagcc gatatagctg
     1081 acctggatcg gcgccgcgcg gcgcgcgaac acgccgagcc gggaatcggc ggtgtggccg
     1141 gtcaggtcga cggcgatgtc ggttttcagc gcgcgcagcg cctcgaccac ggccgcatcg
     1201 ctcatcgcgc gggcgtcgat gaagcggtcg aacgaggcgg cgatgcgctt gcgcatcggc
     1261 ccgccgtcgt cgcggccaaa ggagacgccg acgatctcga aggcggcccg gtcatgcagc
     1321 tcgaacaatt cggcgatcag ataggcgatg gcgtgctcgc ggaaatccgc cgaaagatag
     1381 gcgatgcgaa tcttagcgcc gcgcggcgcg agcggcggct tcgacgacac gacgggcgcg
     1441 gccgcggcga cataaagccg cgcgcattgg agctgcagcg ccggatcgcc gctgaagccg
     1501 agcacagtga acggcgagac gatcgagcgg ccggcgcgaa tatccgccgc gagcgcctgc
     1561 tcgtattcgc cgcgcttcgt ccagtcgcaa agctgcagga cgcaattggc ggcgcgctcg
     1621 agcgcataaa ggtggtcggg ccgcgccgcc ctcgtgcgct cgaaatccgc cagcgcctcg
     1681 gcgtaacgct gctgcgcgaa cagcgcgttg ccgcgattgt agagcgcctc gacgaaatcg
     1741 ggcttgagcg ccaaggcctt gtcgaagctc gaagcggcct cggcgaagcg gccgagatcg
     1801 tgcaaggaat tgccgcggtt gtaatgcgcc tcggcgtctt gcgggctgag cgcgatcagc
     1861 gcgtcgaacg tcgcgagcgc ctcggcgaag cggcgcgctt cgtggagcgc cgcgccgcga
     1921 ttggcgagcg cgccaagatg tttggcatcg atggccaacg cgcgctcgta ggcggcgagc
     1981 gcgtcgttca ggcggccgag cgcgcgcaag acattgccgc gattgaaatg ggaagccgcg
     2041 tcgcgcgggt tgacggcgag ggcgcgatcg tagctcgcca gcgcttcctc gcggcgatcg
     2101 agcgcgaaga gagtcgcggc gcgatgggcg tgggcgtcgc catagtcgcg cttcagcgcg
     2161 atcgcccggt cgaaagcggc cagcgcctcg ccgtagcgcc gcagcgcatg caaagcgagg
     2221 ccgcgattgc cgtgcgccgg cggataggcg ccgttcagcg cgacggcgcg ctcggcggcg
     2281 gcgagagcct cttcgaagcg gccgagatcg tgcaaagccg cgccgcgatt gacgaccgcc
     2341 tcggcatggt tcggctgcaa ggcgagcgcg cggtcgtagc tttgcagcgc cttgtcgccg
     2401 cggcccatcg ccgcctgcac gaagccgagc agatgccagg cgtcgaacag gtccggcttg
     2461 gcggcggcga cggcgcggct gagccgctcc gcctcgccga gatcgccgct catataagcg
     2521 gcgaaagcgc ggcgcagctt ttcaggcggc ggcgcgggca tggcgctcca tgctgctcgc
     2581 aaggacgatt ttcatgcggc ccgtttagcg gcttgtgtct ggattcagca accgatttcc
     2641 cggggctacg gcgcttgtag ccgcagtcgc tgaccgcggc cgaggcgcga ggcgctccgt
     2701 ttaccgctaa agcggcggcg gaagcggcgc ggtcggcgac cgcgcctcca tgaaaggcgc
     2761 gtccgccagc cccttcgtcc cgacatcgac cgcgaacatc gcgccggctt ccggctcggc
     2821 tcggagcttc gccgcatcga gcccgccgcg cgcgctggtg acgaacagcg tcgcgaggtc
     2881 cttgccgccg aaggcgcagc aggtgatttg cgagcaaggc agcgcgacga tgcggtcgat
     2941 ccggccgtcc ggcgcata
//
LOCUS       DATGJW010000338         2527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_272004, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000338 DATGJW010000000
VERSION     DATGJW010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2527
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_272004"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..332
                     /gene="opgC"
                     /locus_tag="VKU03_10390"
     CDS             <1..332
                     /gene="opgC"
                     /locus_tag="VKU03_10390"
                     /inference="COORDINATES: protein motif:HMM:NF021633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="OpgC domain-containing protein"
                     /protein_id="HLJ71718.1"
                     /translation="YVIAQTPRLAALAKSGLGQAFQQLGRHSLAIFALGSVLSCLGQA
                     TLSVAEAQFSLGAEIVGPLYTLFGIMGLFLFVRYLEWNKLSAERRLVPAGNSLSTAWP
                     ASPRPSS"
     gene            455..1057
                     /locus_tag="VKU03_10395"
     CDS             455..1057
                     /locus_tag="VKU03_10395"
                     /inference="COORDINATES: protein motif:HMM:NF024863.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HLJ71719.1"
                     /translation="MDILAIGSSSTQGIGASAPSLTYPAQLQADLAAALRQRVVVQNA
                     GVGGETVAATLARLKALLKREKPDLIIWQVGTNDAVRGEDEATFRATIERGVGIARDA
                     SVPLVLLDQQYFPAIKDIDRYERFVRAVHEVGAEYHDPVFPRYALMKAWAAESPALLR
                     AMLWKDGFHMSDRGYGCLAQDLAADMAPLLRPRLNMTAIK"
     gene            1167..>2527
                     /locus_tag="VKU03_10400"
     CDS             1167..>2527
                     /locus_tag="VKU03_10400"
                     /inference="COORDINATES: protein motif:HMM:NF024882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA translocase FtsK 4TM domain-containing
                     protein"
                     /protein_id="HLJ71720.1"
                     /translation="MRTTSFSVIDYLPDSVRELPKRRAAELIGASVLAGVGALALALL
                     TWSVEDPSLNHAASTPIHNLLGAPGAIAADIAMQALGLSCVAALTPPSFWAWRLLTHR
                     RLERARIRLTLWLLGSMAAAGLAAMLPAPYRWPLPTGLGGVLGDAMVNAARALGARST
                     LGMFLFGGSCAAFAILALTAAAGYGLMRAPEAEADERAAPAAPRRAVHEDEEDDADGE
                     PGFGLISLGAAIHAALSLRGALRRAFSRRKPARPPAARAPWLDPNAKPFTAADDPGPA
                     LPNGRIEPSLMRRASAPAEAAESEAAASAPSRVAAPAGALKPGRRLQSEAQPSFFDRA
                     AYQLPPLIMLAEPKRLVAAKISEDALEQNARLLEGVLDDFGVKGEIINVRPGPVVTLY
                     ELEPAPGIKSSRVIGLADDIARSMSAISARVAVVQGRNAIGIELPNQRRETVYLRELL
                     ASQ"
BASE COUNT          362 a          935 c          819 g          411 t
ORIGIN      
        1 cctatgtgat cgcgcaaacg ccgcgcctgg cggcgctggc caagagcggc ctcggccagg
       61 cgttccagca gctcggccgt cacagtctgg ccattttcgc gctcggctcg gtcctttctt
      121 gcttgggaca agcgacgctc agcgtcgccg aggcgcagtt ttcgcttggc gcggagattg
      181 tcggtcctct atacacgctc ttcggaatca tggggctgtt cctgttcgtc cgatatttgg
      241 aatggaacaa actaagcgcg gagcggcggc tcgtaccggc cggaaattct ctttcgaccg
      301 cctggccggc ctcgcctcgg ccgtcgtcct agcttttcca atcgcttcgg ccgtcccggc
      361 gagggccggc gacggcgact gcccgggctc acgcccgcct ttcgttctcc ccgccccttc
      421 gaacgccaag ggcgaagcca tcgtcgacgg aacggtggac attctggcga tcggctcctc
      481 ctcgacgcaa gggatcggcg cctcggcgcc gagcctgacg taccccgccc agctccaggc
      541 cgatctcgcc gcggcgttaa gacaaagagt cgtcgtccag aacgccggcg ttggcggcga
      601 gaccgtcgcg gcgaccttgg cccggctgaa agccttgctc aaacgggaaa agccggacct
      661 catcatctgg caggtcggca ccaacgacgc ggtgcgcgga gaagacgagg cgacgtttcg
      721 cgccacgatc gaaaggggcg tcggaatcgc ccgcgacgcc agcgtccctc tggtgctgct
      781 cgaccagcaa tatttccccg ccatcaagga catcgaccgg tatgagcgct tcgtgcgcgc
      841 cgtccacgag gtcggcgccg aatatcacga cccagtcttt ccccgttacg ctctgatgaa
      901 agcctgggcc gccgaatcgc cggctttgct gcgcgccatg ctatggaagg acggcttcca
      961 tatgagcgac cgcggctatg gctgcctggc gcaagacttg gccgccgaca tggcgccgct
     1021 gttgcggccc cgtctcaaca tgacggcgat caagtgaggg cgccggccgc ccattaaggt
     1081 taggcgggca ttaactcgcc cggcctagct tgtccgccga ccggcgcggc gcgccggcgc
     1141 ctttaaaaag ggctggcgcg gacgcgatgc gaaccacgag tttctcggtc attgattatc
     1201 tgcccgactc ggttcgcgaa ttgccgaaga ggcgcgccgc cgaactgatc ggcgccagcg
     1261 tgctggcggg cgtcggcgcc ctggcgctgg ctttgctcac ctggtccgtc gaggatccga
     1321 gcctcaacca cgccgcttcc acgcctatcc acaatctcct cggcgcgccg ggggcgatcg
     1381 ccgccgacat cgccatgcaa gcgctcggcc tctcctgcgt ggcggcgctg acgccgccgt
     1441 ccttttgggc ttggcggctc ttgacccatc gccggctcga gcgcgcccgc atccggctga
     1501 cgctctggct gctcggctcg atggccgccg ccggcctcgc cgccatgctg ccggcgccgt
     1561 atcgctggcc gctgccgacc ggccttggcg gcgtgctcgg cgacgccatg gtgaacgcgg
     1621 cgcgggcgct gggcgcgcgc tcgaccctcg gcatgttcct gttcggcggc tcctgcgccg
     1681 ccttcgccat tctcgccctc accgccgccg ccggttacgg cctgatgcgg gcgcccgagg
     1741 ccgaggcgga cgagcgggcc gcgcccgccg cgccccgccg cgccgtccac gaggacgaag
     1801 aggacgacgc cgacggcgaa cccggcttcg gcttgatttc gctgggcgcg gcgatccatg
     1861 cggctttgag cctgcgcggc gcgctccgcc gcgccttcag ccgccgcaag ccggcgcgcc
     1921 cgccggccgc ccgcgcgccc tggctcgatc ccaacgccaa acccttcacg gcggccgacg
     1981 atcccggccc cgccttgccg aatggccgga tcgagccaag cctgatgcgc cgcgcgtcgg
     2041 cgcccgccga ggcggccgaa agcgaagccg ccgcctccgc gccttcgcgc gtcgccgcgc
     2101 cggccggcgc gctcaagccg ggccggcgcc tgcagagcga agcccagcca agctttttcg
     2161 accgcgccgc ctatcaattg ccgccgctca tcatgctggc cgagccgaag cggctcgtcg
     2221 ccgcgaaaat ctcggaagac gcgctcgagc agaacgcccg cctgttggag ggcgtgctcg
     2281 acgatttcgg cgtcaagggc gagatcatca atgtccgccc cggcccggtc gtgacgctct
     2341 acgagctcga gccagccccg ggcatcaaat cctcgcgcgt catcggcctc gccgacgaca
     2401 tcgcccgctc gatgtcggcg atctccgcgc gcgtcgcggt ggtccagggg cgcaacgcca
     2461 tcggcatcga attgcccaac cagcgccgcg agaccgtcta tttgagagaa ttgctcgcga
     2521 gccagga
//
LOCUS       DATGJW010000339         2208 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_377406, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000339 DATGJW010000000
VERSION     DATGJW010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2208)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2208)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2208
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_377406"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(99..917)
                     /locus_tag="VKU03_10405"
     CDS             complement(99..917)
                     /locus_tag="VKU03_10405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008140269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71721.1"
                     /translation="MTSLSLRRIGAMVARYLYVLRSSWPRIAELIYWPAIQMFTWGFL
                     QTYINGQASAAGLAGRFAVAAGTLIGALLLWDSLFRAQLGFSLSFLEEMWSRNMANLL
                     MSPLRPFEFVAALMAMSLIRLAIGILPVTGLAFLFFGFNLWGLGLAVAAFFANLVLTS
                     WAIGLFVSGLVLRNGMGAESIAWSILFFVMPFACVYYPVATLPHWLQCVAWSLPPTYV
                     FEGLRALLVAHAFRADLMLEGFGINAFLLAFGAVAFLALLDSARRAGSLLQMGE"
     gene            complement(914..1666)
                     /locus_tag="VKU03_10410"
     CDS             complement(914..1666)
                     /locus_tag="VKU03_10410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615250.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71722.1"
                     /translation="MISAPPVAAAISARALRKTFKKVVAVDSVSFDIALGSTTALLGG
                     NGAGKTTTIGMIMGLILPTSGRVSVLGADMARERHRVLARMNFESPYVDMPHRLTVRQ
                     NLKVFAGLYGVADIAMRIDELAERLALEEFLDRQAGKLSAGQKTRVALAKALINEPDV
                     LLLDEPTASLDPDTADWVRSRLEDYRRKRGATILLASHNMAEVERLCDRVIMLKTGRV
                     VDDGAPAALIAQYGRDNLEQVFLDIARGRGAS"
     gene            complement(1690..>2208)
                     /locus_tag="VKU03_10415"
     CDS             complement(1690..>2208)
                     /locus_tag="VKU03_10415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012041159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxychromene-2-carboxylate isomerase"
                     /protein_id="HLJ71723.1"
                     /translation="PVALNEVFAETGGLPLAKRHPARQAYRMVELQRWREKRGLAFHL
                     RPKHWPFDANLANCAVLAIVRAGGDPAGFVQSAFAAIWEQEENLGEEAVLSRLLGEAG
                     FNPADILAAARSAETLGVYERNRLDAIAIGAFGSPCYVLNGEVFWGQDRLDLLEDALK
                     SGRPPYSATPAA"
BASE COUNT          437 a          704 c          751 g          316 t
ORIGIN      
        1 ggctaagacg cgccacttcc tgaaaaatct gttggaaaca agcttaacgg ggggtgaatg
       61 gcgccatggg gccgccattt ccttgccgcc gccgcgtctt attcgcccat ctggagcaag
      121 gagccggcgc ggcgcgcgct atccaggagc gcgaggaaag cgacggcgcc gaaagcgagg
      181 aggaaggcgt tgatgccgaa gccttcgagc atcaggtcgg cgcggaacgc gtgggcgacg
      241 agcaaagcgc gcaaaccctc gaagacgtag gtcggcggca ggctccaggc gacacattgc
      301 aaccaatgcg gcagcgtcgc caccgggtaa tagacgcaag cgaaaggcat gacgaagaac
      361 aggatcgacc aggcgatcga ttcggccccc atgccgttgc gcaggacgag gccggagacg
      421 aagagcccga tcgcccagct cgtcaggacg agattggcga agaacgccgc gacggcgagg
      481 ccgagccccc aaaggttgaa gccgaagaac aggaaggcga gcccggtcac cggcagaatg
      541 ccgatggcga ggcggatcag gctcatcgcc atcaaagcgg cgacgaattc gaacggccgc
      601 aacgggctca tcaacagatt ggccatgttg cgcgaccaca tctcttcgag gaaggacagc
      661 gagaagccga gctgggcgcg gaacaggctg tcccagagca gcaacgcgcc gatcagcgtt
      721 ccggcggcga cggcgaagcg cccggccagg ccggcggcgc tggcctggcc gttgatgtag
      781 gtctgcagga agccccaggt gaacatctgg atcgccggcc aatagatcag ctcggcgatg
      841 cgcggccagg acgagcgcag cacatagaga taacgggcga ccatcgcgcc gatgcgtcgc
      901 agggagaggc tggtcatgac gcgccgcgcc cgcgcgcaat atcgagaaac acttgttcga
      961 gattgtcgcg gccatattgg gcgatcagcg cggccggcgc gccatcgtcg acgacgcggc
     1021 cggttttcag catgatcacg cggtcgcaga gccgctcgac ctcggccata ttatgcgacg
     1081 cgagcaggat cgtcgcgccg cgcttgcgcc ggtagtcttc gagccgcgag cgcacccaat
     1141 cggcagtgtc gggatcgagc gaggcggtcg gctcgtcgag cagcagcacg tccggctcgt
     1201 tgatcaaagc cttggcgagc gcgacgcgcg tcttctgtcc cgccgacaat ttcccggctt
     1261 ggcggtcgag gaactcctcc aaggccaggc gctcagccaa ttcgtcgatc cgcatggcga
     1321 tgtcggcgac gccgtagagg ccggcgaaga ctttcaggtt ttgcctgacg gtgagccgat
     1381 gcggcatgtc gacatagggg ctctcgaaat tcatccgcgc gaggacgcga tggcgctcgc
     1441 gcgccatgtc ggcgccgagc acgctgaccc gcccggaggt cggcaggatg agccccatga
     1501 tcatgccgat cgtcgtcgtc ttgccggcgc cgttgccgcc aagcagcgcc gtcgtcgagc
     1561 caagcgcaat gtcgaaggag acggaatcga cggcgacgac tttcttgaac gtcttgcgca
     1621 aggcgcgcgc cgagatcgcc gcggcgaccg gcggcgctga aatcatgctc gccgaatgaa
     1681 cggtttcatt cacgccgcgg gcgtcgcgga ataaggcggt cggccgcttt tgagcgcgtc
     1741 ctccaaaagg tcgagccggt cctgccccca aaagacttcg ccgttcagca cgtaacaggg
     1801 cgagccgaac gcgccgatgg cgatggcgtc gaggcgattg cgctcgtaaa cgccgagcgt
     1861 ctcggcgctc ctcgccgcgg caagaatatc cgccgggttg aagccggcct cgccgagcag
     1921 gcgggacagg actgcttcct cgccgagatt ctcctcctgc tcccagatcg cggcgaaagc
     1981 cgattgcacg aagccggccg ggtcgccgcc cgcccggacg atcgccagca cggcgcaatt
     2041 ggcgagattc gcgtcgaacg gccagtgctt gggccgcaaa tgaaaggcaa gcccgcgctt
     2101 ctcgcgccag cgctgcaatt cgaccatgcg ataggcctgg cgcgccggat ggcgcttggc
     2161 gagcggcaac ccgcccgtct cggcgaagac ctcgttcagc gccaccgg
//
LOCUS       DATGJW010000340         2282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1513399, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000340 DATGJW010000000
VERSION     DATGJW010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2282
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1513399"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..679)
                     /locus_tag="VKU03_10420"
     CDS             complement(<1..679)
                     /locus_tag="VKU03_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018483742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HLJ71724.1"
                     /translation="MAAVQETVDKVRAIDVSEYKYGFVTDIESEKAPKGLNEDIVRFI
                     SGKKAEPEWLTEWRLEAYRRWLTMDEPKWARVEYPPIKYQELYYYAAPKASAGPRSLD
                     EVDPELLRTYEKLGIPLREQEVLAGVEKPRVAVDAVFDSVSVATTFKAELAKAGVIFS
                     PISEAVRTHPDLVRKYLGSVVPTSDNFFATLNSAVFSDGSFVYVPPGVRCPMELSTYF
                     RINERNTG"
     gene            complement(754..1122)
                     /locus_tag="VKU03_10425"
     CDS             complement(754..1122)
                     /locus_tag="VKU03_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002969201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ71725.1"
                     /translation="MFDHLGFAAADFAKSKAFYEKALAALGIGVVMHGEDFVGFGRDG
                     RPQFWVGVGNAPTTRLHLAFAAENRSSVRAFYEAALAAGGKDNGAPGLRPDYHPNYYG
                     AFVIDPNGHNIEAVCHSPEA"
     gene            complement(1143..>2282)
                     /locus_tag="VKU03_10430"
     CDS             complement(1143..>2282)
                     /locus_tag="VKU03_10430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HLJ71726.1"
                     /translation="NATAPLRPAARAELLASLDLTGNPSSVHSEGRAARRAVERARAE
                     VAALVAGEARNATFVASGTEAANLALTPHIDDRGSAGPLSRLIVSAGEHSCVLSGHRF
                     APAAVETAPLESTGRIDLDVLETLVARAAGGRVALALQAANNETGVIQPVAAAAALVH
                     RIGGIVICDAVQLAGRERCDFAALGADFLLLSGHKLGGPKGAGALVAASDALRIAEPL
                     VRGGGQERGLRAGTENVAAIAGFGAAARAALADLDAEAERLRALRDALEGHVLGAAPD
                     AVVFGAGVDRLPNTLCFAIPGVAAETLLIGLDLAGFAVSSGAACSSGKVARSHVLTAM
                     GVEPALAAGAVRLSLGWASTAEEAARFGEAFGRILAPMRRGRSAA"
BASE COUNT          404 a          794 c          739 g          345 t
ORIGIN      
        1 ggccggtatt gcgctcgttg atccggaagt aggtcgaaag ctccatcggg cagcgcacgc
       61 cgggcggcac atagacgaag gagccgtcgg agaacacggc cgagttcagc gtcgcgaaga
      121 aattgtcgga ggtcggcacg acggagccga gatatttcct caccagatcg ggatgcgtgc
      181 gcaccgcctc ggaaatcggg ctgaagatca cgccggcctt ggcgagctcg gccttgaacg
      241 tcgtcgccac cgagacggaa tcgaacaccg cgtcgaccgc cacgcgcggc ttctcgaccc
      301 cggccagaac ttcctgctcg cggagcggga tgccgagctt ctcatacgtg cgcagcagct
      361 cgggatcgac ctcgtcgagc gagcgcggcc ccgccgacgc tttcggcgcg gcatagtaat
      421 agagctcctg atatttgatc ggaggatatt cgacccgcgc ccatttgggc tcgtccatgg
      481 tcagccagcg ccgataggcc tccagccgcc attcggtcag ccattccggc tcggctttct
      541 tgccggaaat gaagcggacg atatcctcgt tgaggccttt cggggccttt tcggattcga
      601 tgtcggtgac gaagccgtat ttatactcgc tgacgtcaat cgcgcgaact ttgtcgaccg
      661 tctcttggac cgccgccatc tagaactctc ctcgccttgg cggtttcaag gaccgccggt
      721 gggactgcag caatgttgcc ggaccgcttt cgctcacgcc tccgggctat ggcacacagc
      781 ctctatattg tgtccgttgg ggtcgattac aaaggcgccg taataattcg gatgataatc
      841 ggggcgcaag ccgggagcgc cgttatcctt gccgccggcg gcgagcgccg cctcgtagaa
      901 ggccctcacc gaggagcggt tttcggccgc gaaagcaagg tgcaggcgcg tcgtcggcgc
      961 attgccgacg ccgacccaga attgcggcct gccatcgcgg ccgaacccga cgaaatcctc
     1021 gccgtgcatg accacgccga ttccgagcgc ggccaaggcc ttctcgtaaa aagccttgct
     1081 cttcgcaaaa tcggcggcgg cgaaacccag gtgatcgaac atcgttcgga ctcgtccttc
     1141 tgttatgcgg cgcttcggcc gcggcgcatc ggcgcaagaa tgcggccgaa cgcttcgccg
     1201 aagcgcgccg cctcctcggc ggtcgacgcc caaccgagac tcagcctgac cgccccggcg
     1261 gcgagcgccg gctcgacccc catggcggtc aatacatgcg agcgcgcgac cttgcccgac
     1321 gagcaggccg ccccggacga gacggcgaaa cccgcaagat cgaggccgat cagcaacgtc
     1381 tcggcggcga cgcctggaat ggcgaagcag agcgtattgg gcagccggtc gaccccggcg
     1441 ccgaaaacga ccgcgtccgg cgcggcgccg aggacatggc cctccagcgc gtcgcgcaaa
     1501 gcgcggagcc tttccgcctc ggcgtcgaga tcggccagcg cggcgcgcgc cgccgcgccg
     1561 aaaccggcga tcgcggcgac attctccgtc cccgccctga ggccgcgctc ctggccgccg
     1621 ccgcgcacga gcggctcggc gatgcgcaac gcgtcggagg cggcgaccag cgcgccagcg
     1681 cctttcgggc cgccgagctt atggccggac aacaatagaa aatccgcgcc gagcgcggca
     1741 aaatcgcagc gctcccggcc ggcgagttgg accgcgtcgc agatgacgat cccgccaatc
     1801 cgatgaacca gagcggccgc ggcggccaca ggctggatga cgcccgtctc gttgttcgcc
     1861 gcctgcaggg cgagcgcgac gcgcccgccg gcggcgcgcg caacaagcgt ctccagcaca
     1921 tccaaatcga tccggccggt cgactcgagc ggcgccgttt cgaccgccgc gggcgcgaaa
     1981 cgatggccgc tcaacacgca ggaatgctcg ccggcggaga cgataagccg cgatagcggc
     2041 cctgccgaac cgcgatcgtc aatatgcggc gtcagcgcaa gattggccgc ctcggtgccg
     2101 ctcgcgacaa aggtcgcgtt gcgcgcctcc ccggcgacca gggcggcgac ttccgcccga
     2161 gccctctcca ccgcccgccg ggccgcccgc ccttctgaat ggacggatga aggatttccg
     2221 gtaagatcaa gcgacgcaag caattcggcg cgagccgccg ggcgcaacgg cgccgtcgcg
     2281 tt
//
LOCUS       DATGJW010000341         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_996297, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000341 DATGJW010000000
VERSION     DATGJW010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996297"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1146)
                     /locus_tag="VKU03_10435"
     CDS             complement(<1..1146)
                     /locus_tag="VKU03_10435"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLJ71727.1"
                     /translation="MLQASRATTSLSGPAAQARPAAAFASKESAVAAIVILGFAAARI
                     VVAFLLDFGIDEAYTTTIARRLDWSYFDHPPLHQWIAHFAALALGENILVRLPFIALF
                     AATGWLIFALTRDLFGAAAGLWAVFALNATPFFFASAGQWILPDGPLLFALAAAAFAV
                     GRLFFRQDAAAWPLWLATGFSLGLAGLSKYNAVFFPVGLVLFLVASPQNRRWLAHPAP
                     YCAALLAVAMVAPVILWNADNGWVSFLFQGERGAASGHWRPAQAGAMALSEVAFLSPW
                     IFAPLLAGLIEAGRGALSGNADGGRRLFLLCLALPPIMFFTLTPLWGARGLPHWPMPG
                     WLFAYPLLGVWLAPRASLARRGWAIGSAAALALVALIAVAEARTGFLM"
     gene            1309..1740
                     /locus_tag="VKU03_10440"
     CDS             1309..1740
                     /locus_tag="VKU03_10440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71728.1"
                     /translation="MLALILAAAVSTSSIPSLDISTGCKPQGSVEGQITDYKSCMQDE
                     EATKQKLTKEWASYPIAARRECVSDRPSLVNSYVELLTCIEMETWKSEPAVTNPSGEA
                     AGAAMGARPAGALGGSPPTPGQIGGASIAHPLGGNPAIHIH"
     gene            complement(1753..>2233)
                     /locus_tag="VKU03_10445"
     CDS             complement(1753..>2233)
                     /locus_tag="VKU03_10445"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HLJ71729.1"
                     /translation="EENLMAAFQSRVSMEDFANRAPAVPAALRRLGEAIEASGLDKTL
                     TELVKLRASQLNHCAFCVQFHLNVLRKLGAEQEKLDLVAVWREAGVFSEREAAALAWT
                     EALTLSPGHNATDEAYAALLTQFTEQEAIFLTAAIGLINAWNRIGVGLRFAPPVPAR"
BASE COUNT          473 a          713 c          764 g          283 t
ORIGIN      
        1 catgaggaat ccggttcggg cctcggcgac ggcgatcaag gcgacgagcg ccagcgccgc
       61 ggccgaaccg atcgcccagc cgcggcgcgc gagggaggcg cgcggggcga gccagacgcc
      121 gagcagcgga taggcgaaga gccagcccgg catcggccaa tgcggcaagc ccctggcgcc
      181 ccatagcggc gtcagcgtga aaaacattat cggcggcaga gcgaggcaga gcaggaacag
      241 tcgccggccg ccatcggcgt ttcccgacag tgcgccgcgc ccggcctcga tcaatccggc
      301 cagcagcggc gcgaaaatcc acggcgacag aaacgcgacc tcgctcagcg ccatcgcgcc
      361 ggcctgcgcc ggccgccaat ggccggaagc cgcgccgcgc tcgccttgga aaaggaacga
      421 gacccagccg ttgtcggcgt tccaaagaat gaccggcgcg accatcgcga cagcgagcag
      481 agcggcgcaa tagggcgccg gatgagcgag ccagcggcga ttctgaggcg aagcgacaag
      541 gaaaaggaca aggccgacgg gaaaaaatac ggcgttgtat ttcgacaggc cggcaaggcc
      601 caggctgaag cccgtcgcca gccatagcgg ccaggctgcg gcgtcctgcc gaaagaacag
      661 gcggccgacc gcaaaggccg cggcggccag cgcgaagagg agcgggccgt cgggcaagat
      721 ccattgaccc gccgaggcga aaaagaaagg cgtcgcattc agcgcgaaaa ccgcccaaag
      781 gccggcggcc gcgccgaaaa gatcgcgcgt cagcgcgaag atgagccagc cggtcgcggc
      841 aaacagagcg atgaagggca gccggacaag gatgttctcg ccgagcgcga gcgcggcgaa
      901 atgcgcgatc cattgatgca gcggcggatg gtcgaaatag gaccaatcga gccgccgcgc
      961 gatcgtcgtc gtataggctt cgtcaatgcc gaaatcgagc aagaaggcga cgacgatccg
     1021 cgccgccgca aatccgagaa tgacgatcgc ggcgacagcg ctttccttgc tcgcgaaggc
     1081 ggccgccggc cgcgcctgcg cggcgggacc gcttaaggaa gtcgtcgcgc gagacgcttg
     1141 caacacaggc gaagcccctt gacccgggtc gagccccgag ccgacaggaa atggagtgaa
     1201 ccaacgcagc ctgtcaatca gccgcgtggg gcgggcggag cgccgaacat tttgtcggcc
     1261 cgtcgcgttt gcgcgataat ggagccgcgt gaacgagcgg aggattcaat gctggcgtta
     1321 attcttgccg ccgcagtctc gacgagctcc attccatccc tggatatctc gaccggctgc
     1381 aagcctcagg gctcggtcga gggccagatc accgactata aaagctgcat gcaggacgag
     1441 gaggcgacca agcagaagct gaccaaggaa tgggcttcct acccgatcgc cgcgcgccgg
     1501 gaatgcgtgt cggatcgacc gagcctcgtg aacagctatg tcgaattgct gacctgcatc
     1561 gaaatggaaa cctggaagag cgaaccggcg gtgaccaatc cgagcggcga ggcggcgggc
     1621 gcggcgatgg gcgccaggcc tgccggcgcg cttggcggat cgccgccgac gccggggcaa
     1681 atcggcggcg cgtcgatcgc tcacccgctt ggcggcaacc cagccatcca tattcactga
     1741 gggacgggcg ggtcagcgag cgggaacggg cggcgcgaag cgcaagccga ccccgattct
     1801 gttccaagcg ttgatcaatc cgatcgccgc ggtgagaaag atcgcttcct gctccgtgaa
     1861 ttgagtgagc aaagccgcat aggcctcgtc ggtcgcgtta tggccgggcg acagcgtgag
     1921 cgcctccgtc caagcgagcg ccgccgcctc acgctcggag aagacgcccg cctcgcgcca
     1981 gacggcgacg agatcgagct tttcctgctc ggcgccgagc ttgcgcagaa cattgagatg
     2041 aaactgcacg caaaaggcgc agtggttcag ttgcgaggcc ctgagcttga cgagttcggt
     2101 caaggtcttg tcgaggccgg acgcctcgat cgcttctccc aggcgccgca aggcggccgg
     2161 caccgccggc gcgcggttcg caaaatcctc catgctgacg cgcgattgaa aggccgccat
     2221 caaattctcc tct
//
LOCUS       DATGJW010000342         2074 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_996765, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000342 DATGJW010000000
VERSION     DATGJW010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2074)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2074)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2074
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996765"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1816)
                     /locus_tag="VKU03_10450"
     CDS             complement(134..1816)
                     /locus_tag="VKU03_10450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLJ71730.1"
                     /translation="MNQSTNLRTGGRILVDQLVRQGVEHVFCVPGESYLAALDAMYDA
                     NLKVTVCRQEAGATMMAAASAILTGRPGICFVTRGPGAANAAHGLHIAEHDSTPLILF
                     IGQVERGMLGRGAFQEMDYRAFLGSTVKWATQIEDAARIPEIIQRAFHVAMQGRPGPV
                     AIALPEDVLTDMAAAADALRVEPAPIWPGLTQMAELQKMLWAAKRPIAIVGGGGWDQR
                     ATQALRRFAERFDLPVAASFRRGSLFDGEHKNYAGEIGIGPNPKLKARIADADLVVLI
                     GGRMAEMPSQSYTLFEIPTPRQRLVHVHPDPHEIGRVYHPTLGIVATAPAFCAALEGL
                     QPPNAIAWSEATRKAHAEYLEWTDKAPAMPGRMQLGEAMLWLRGRLDEDVIVCNGAGN
                     YAAWPGRFLRYRKIGAQIGPTSGSMGYGVPAAVGAKRVRPDRMVVSFNGDGCFLMNGQ
                     EFATAVQYDLAIVFIVIDNGMYGTIRMHQEREYPGRISATALKNPDFAAYARAFGGHG
                     ETVEETSQFAPAFERAVASGKPAILHVKIDPDAISPTTTLSAIRAKALAGGK"
BASE COUNT          337 a          691 c          702 g          344 t
ORIGIN      
        1 caactgactt tctcctgagc ctgctttggc gagcgacgcg cgaagcgcgc ttggccttga
       61 cggcgagcac ggctcaggca caccgcgctt caagatgcgt gcatgccgta gccgcgaagc
      121 ggggagaacg gatctacttc ccgccggcca gcgccttggc gcggatcgcc gaaagcgtcg
      181 tcgtcgggga gatcgcgtcg ggatcgatct tgacatggag gatcgccggc ttgcccgagg
      241 cgacggcgcg ctcgaaagcc ggcgcgaact ggctcgtctc ctccactgtc tcgccatggc
      301 cgccgaaagc gcgggcatag gcggcgaaat cgggattctt cagcgcggtc gcgctgatcc
      361 ggcccggata ttcgcgctcc tgatgcatcc gaatggtgcc gtacatgccg ttgtcgatga
      421 cgatgaagac gatggcgaga tcgtactgaa ccgcggtggc gaattcctgc ccgttcatca
      481 ggaagcagcc gtcgccgttg aaggagacga ccatccgatc ggggcggacg cgcttggcgc
      541 cgaccgccgc cggcacgcca tagcccatcg agcccgatgt cggcccgatc tgcgcgccga
      601 ttttgcggta gcgcaggaat cgccccggcc atgcggcgta attgccggcg ccgttgcaga
      661 cgatgacgtc ctcgtcgagc ctgccgcgca gccacagcat cgcctcgcca agctgcatcc
      721 tgcccggcat cgccggcgcc ttgtccgtcc attccagata ttccgcatgc gccttgcgcg
      781 tcgcctcgct ccaggcaatc gcgttcggcg gctgcaagcc ttcgagcgcg gcgcaaaagg
      841 cgggcgcggt cgcgacgatc ccgagcgtcg ggtgatagac gcggccgatc tcgtgcgggt
      901 cgggatggac atggacgagg cgctggcgcg gcgtcggaat ctcgaacaag gtgtagctct
      961 gcgacggcat ttccgccatc cgcccgccaa tcagcacgac aagatcggcg tccgcgatgc
     1021 gcgccttgag cttcggattg gggccgatgc cgatctcgcc ggcatagttt ttgtgctcgc
     1081 cgtcgaacag cgagccgcgg cggaacgagg cggcgaccgg caggtcgaag cgctcggcga
     1141 agcgccgcag ggcttgcgtc gcgcgctggt cccaaccgcc gccgccgaca atcgcgatcg
     1201 gccgcttggc ggcccagagc attttctgca attcggccat ttgcgtcagg ccgggccaga
     1261 tcggcgcggg ctcgacgcgc agggcgtcgg ctgccgcggc catatcggtg agcacatcct
     1321 ccggcaaagc gatggcgacc ggcccaggcc ggccttgcat cgccacatga aaggcgcgct
     1381 ggatgatttc cgggatgcgc gcggcgtcct cgatttgcgt cgcccatttg accgtcgagc
     1441 cgagaaaggc gcgatagtcc atctcctgga acgcgccgcg gccgagcatg ccgcgctcga
     1501 cctggccgat gaacaggatc agcggcgtcg aatcgtgctc cgcgatgtgc aggccatgcg
     1561 cggcgttggc cgcgccgggc ccgcgcgtga cgaagcagat accgggccgg ccggtcagga
     1621 tcgcgcttgc cgccgccatc atcgtcgcgc cggcttcctg gcggcagacc gtcactttga
     1681 ggttcgcgtc gtacatcgcg tcgagcgctg cgaggtacga ctcgcccggc acgcagaaga
     1741 cgtgctcgac gccttgccgg acgagctgat cgacaagaat gcgcccgccc gtgcgcagat
     1801 tggtggattg attcatcggt gtggcgcttc cctgctcagg agagtcttat tcggcgaggc
     1861 tgcggcttcc cctcacccca accctctccc gcaagcggga gagggggcgc cgcggcttga
     1921 tcgacgcggc tagaaaccct tctcccggtt accggagaag gtggcgcgaa gcgccggatg
     1981 agggcaagcc gcggcactaa tcattcctgc gccaatcggg cgaccctggt taagagaacg
     2041 cctgcaatcc ggtctgcgct cggccgagga tcag
//
LOCUS       DATGJW010000343         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_996770, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000343 DATGJW010000000
VERSION     DATGJW010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996770"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(252..893)
                     /locus_tag="VKU03_10455"
     CDS             complement(252..893)
                     /locus_tag="VKU03_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334556.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphotransferase family protein"
                     /protein_id="HLJ71731.1"
                     /translation="MTIFTLDGLDLASLACSRVCHDVISPVGAIINGLEVLEEEKDAE
                     MRQVALDLIKKSAATASARLQFCRLAYGAAGSAGASIDTSDAENVTRGLIGDERTKLV
                     WNVPRQLAPKNKVKLLLNLCQLAFSAIPRGGVVTVTVTGEGETIEAEIEAKGLNARLA
                     KGAAALIAGEPESGAIDAHTIQPFFAGVVARACGMAVAVETEAEIVRLRARPA"
     gene            1045..1911
                     /gene="cysQ"
                     /locus_tag="VKU03_10460"
     CDS             1045..1911
                     /gene="cysQ"
                     /locus_tag="VKU03_10460"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase CysQ"
                     /protein_id="HLJ71732.1"
                     /translation="MSASGQARDISAPLDLSVAPADVVGEFFAEIALAAGPVVMAEYG
                     EGCQARAKSDMSPVTAADERAEALILERLRAGAAPIAIVAEESAARGEKPALGGCFVL
                     VDPLDGTREFLSRNGEFTINIALVRDHAVAAGAVYAPALGRLWLAGRRACACAVPVGA
                     SLPPAAARRPLKTRAAPRAGLVALASRSHCDPATEAFLAALPIGERRSAGSSLKFCLM
                     AEGEADLYPRFGPTMEWDTAAGEAILRAAGGTVVSAGGGPFLYGKAEAGFRNAGFIAC
                     GDPSLPRRLGHA"
     gene            2054..>2419
                     /locus_tag="VKU03_10465"
     CDS             2054..>2419
                     /locus_tag="VKU03_10465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EipA family protein"
                     /protein_id="HLJ71733.1"
                     /translation="MPNVTRRKILAGAAAGLGAFAAGAARADAPAPFSTEELVDSGHR
                     FFGAISRGLAQVVEEATRRWGQPNGYILGQEASAAFVGGLRYGEGKMYTRNAGDQRVF
                     WQGPSLGFDAGADGDRTMML"
BASE COUNT          368 a          841 c          795 g          415 t
ORIGIN      
        1 aacttcaacc accgcttcag aaggccctgg tccctccatc agcttccggc acttcaagct
       61 aaattcctgc atttttcact cctccgatat agaacgcatg gcgaacagtt tccgcggtcc
      121 atccggcgta tgccgggcgg acgctggtcg aaaattctct cggcccgccg caacagccga
      181 cctcgggaga cgaaaggagg ggacaataat ccttcgcgca aaaatgtccc gcgaatgtga
      241 attatccaaa actacgccgg tctggcgcgc agcctgacga tctccgcctc ggtttcgacc
      301 gccaccgcca tgccgcaggc gcgggcgacg acgccggcga agaacggctg aatcgtatgg
      361 gcgtcgatcg cgccgctttc cggctcgccg gcgatcaaag ccgccgcgcc cttggcgagc
      421 cgggcattga gacctttcgc ctcgatctcg gcctcgatcg tctcgccctc gccggtgacg
      481 gtgacggtca ccacgccgcc gcgcggaatc gccgagaagg cgagctggca aaggttgagc
      541 aaaagcttga ccttgttctt cggcgccaat tggcggggga cgttccagac cagcttggtt
      601 cgctcgtcgc cgatcaggcc gcgcgtgacg ttttcggcgt cgctggtgtc gatcgaggcg
      661 ccggccgagc cggccgcgcc ataggcgagc cggcaaaatt gcagccgcgc cgaggcggtg
      721 gcggcgctct tcttgataag atcgagcgcc acctggcgca tttccgcgtc cttttcctcc
      781 tcgagcactt ccaggccgtt gatgatcgcc ccgaccgggc tgatcacgtc atggcagacg
      841 cgcgagcacg ccaaagacgc gagatccagt ccgtcaaggg tgaaaattgt catcgagcgg
      901 ttcctggcgt cctgatctgc agccgctggc ctcggcgcgc gcgcctgaaa gaccggcgcg
      961 gcgcggagcc gcgcgaatca gccggcgcca gaccatcggc gaggcccatt gcatgagcgc
     1021 cgtcgaacaa gatagcaaga cctcatgagc gcttctggac aagcccgcga tatttccgct
     1081 ccgctcgatc tcagcgtcgc gccggccgac gtggtcggcg aattcttcgc cgagattgcg
     1141 ctcgccgcgg gcccggtcgt gatggccgaa tatggcgagg gctgccaggc tcgcgccaag
     1201 tccgatatga gcccggtcac cgccgccgac gagcgcgccg aggcgctgat cctggagcgg
     1261 ctgcgcgcag gggccgcgcc gatcgcgatc gtcgccgaag aatccgcggc gcggggggag
     1321 aagccggcgc ttggcggctg cttcgttctg gtcgatccgc ttgacgggac gcgcgaattc
     1381 ctctcccgca acggcgagtt caccatcaac atcgccctcg tccgcgatca cgccgtggcc
     1441 gcgggcgccg tctacgcgcc cgccctcggc cgcctctggc tggccggccg gcgcgcctgc
     1501 gcctgcgccg tcccggtcgg ggcgtctttg ccgccggccg ccgcacggcg tccgctcaag
     1561 acccgcgctg cgccgcgcgc cggccttgtc gccttggcga gccgctccca ttgcgatccg
     1621 gccaccgagg cttttctggc cgccctgccg atcggcgagc gccgctcggc cggctcgtcg
     1681 ttgaaattct gcctcatggc cgagggcgag gcggacctct atccgcgctt cgggccgacc
     1741 atggaatggg ataccgcggc cggcgaagcg atcctgcgcg ccgctggcgg gaccgtcgtc
     1801 tcggcgggag gcggcccatt cctctatggc aaggccgaag ccggctttcg caacgcgggc
     1861 ttcatcgcct gcggcgatcc gtctttgccg cgacggctcg gccatgctta acgaacctta
     1921 aagaacatcc gtcgacactt gctttgggac ggctgtgcgg cgccaaacct tcgccacaca
     1981 ggcgacattt tttggttcga cggctgtcgc ggcgcatgat gcgccagctc gtggggcctg
     2041 gagggcatga gcgatgccga atgttacccg gcgtaaaatc ctggcgggcg cggccgccgg
     2101 tttgggcgcc tttgccgccg gcgcggcccg cgccgacgcg ccggcgccgt tttccaccga
     2161 agagctggtc gatagcggcc accgcttctt cggcgcgatc tcccgcggcc ttgcccaggt
     2221 ggtcgaggag gcgacacggc gctggggcca gcccaatggc tatattcttg gccaggaggc
     2281 ttccgcggcc tttgtcggcg gcctgcgcta tggcgaaggc aaaatgtata cgcgcaacgc
     2341 cggcgaccag cgcgtcttct ggcaggggcc gtcgctcggc ttcgacgcgg gcgccgacgg
     2401 cgaccgcacc atgatgctg
//
LOCUS       DATGJW010000344         4874 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_996885, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000344 DATGJW010000000
VERSION     DATGJW010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4874)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4874)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4874
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_996885"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..849
                     /locus_tag="VKU03_10470"
     CDS             <1..849
                     /locus_tag="VKU03_10470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896362.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RimK family alpha-L-glutamate ligase"
                     /protein_id="HLJ71734.1"
                     /translation="PQEEMPPSSLETLRFFEKFAERHSVDIEPITKRQLAELAEFDGL
                     FIRATTSIDNYTYRFARRALQEGMPVIDDPVSMIRCTNKVYLHELMEANGVPIPPTIM
                     LAEEEDLEKAAKALGWPMVVKIPDGSFSRGVHRVENFEALKTLADQLFEDTDLLLAQK
                     FMPTDFDWRVGVLDREPLFVCQYRMARGHWQIIKHGPNGAREGGSTTIALNDAPPEVI
                     DVALRAASAIGDGLYGVDVKQTASEIAVIEVNDNPNLDHGCEDQVGKDEIWSRILQWF
                     VKRIDA"
     gene            1097..1960
                     /locus_tag="VKU03_10475"
     CDS             1097..1960
                     /locus_tag="VKU03_10475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265736.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NlpC/P60 family protein"
                     /protein_id="HLJ71735.1"
                     /translation="MTSVTFDPRLTPARPDLAAAFLRGKVEAARFVEGLRRRVAEPVA
                     PLRRAPAHDAMLETQALYGEEVAVFEENEGWAWVQLQRDSYVGYMPAAALAAEAAPTH
                     RVVVLSTFVYPKPNIKAPPLMALPLDARIEIKAGEGEFCALAGGGFIYARHVAPLGAF
                     EPDFVAVAERFLEAPYLWGGRTAQGLDCSALAQNALAAAGRSAPRDTDMLEAALGSPV
                     AFDQSLAGLKRGDLVFWKGHIGVMRDAAMLLHASGWHMQVVAEPLAGARDRIAAKGGG
                     PITSIRRLSAE"
     gene            complement(1999..2235)
                     /gene="rpmE"
                     /locus_tag="VKU03_10480"
     CDS             complement(1999..2235)
                     /gene="rpmE"
                     /locus_tag="VKU03_10480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
                     /protein_id="HLJ71736.1"
                     /translation="MKADIHPNYHMIKVVMTDGTEFTTRSTYGEPGATLHLDIDSKTH
                     PAWTGGSQQLLDRGGRLSRFNSRFGNLTMGKAKK"
     gene            2481..3140
                     /locus_tag="VKU03_10485"
     CDS             2481..3140
                     /locus_tag="VKU03_10485"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLJ71737.1"
                     /translation="MNWTSAMTRRAAAGLAALGLWLAATATGAQTAAPKPEGFADMAR
                     ALEPGPLPELSVGDKAGVPMIEYGSVTCTHCAHFDKEVWPAIKAAYVDTGKVRYIFRE
                     YSRNSLDVGAFMLARCNGDDKALATIELLFATQDQWAFVDKPLDGLVDVLKQTGLTRD
                     KIETCLKDQGKADALVNIVKTADQNFAVKGTPTFIIDGKIYGGALSKDELDAILKPLV
                     K"
     gene            complement(3375..4013)
                     /locus_tag="VKU03_10490"
     CDS             complement(3375..4013)
                     /locus_tag="VKU03_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017992370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1109 domain-containing protein"
                     /protein_id="HLJ71738.1"
                     /translation="MKTADLIAALAADPKPQGPPLSRRFVLALAAGALISACLFLGMV
                     GPRHDIARALGTIRFDWKFVDTIALAIPTGLATWRLLRPGAERRWLAIALAAPLILLG
                     GSVAMELMMVPPDLWLARLIGSNALHCLTLIPLLSIAPLAALLLAMRAGAPASPPLAG
                     AAAGLVSAGIAATLYATNCTDDSPLFVATWYTLATMIVVAVGAFIGGRVLRW"
     gene            complement(4010..4567)
                     /locus_tag="VKU03_10495"
     CDS             complement(4010..4567)
                     /locus_tag="VKU03_10495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLJ71739.1"
                     /translation="MGEAESDEDWAVRMRAALAGDEATYRRLLEEIGRSVRAMARGAF
                     ARSRSGDADVEDVVQETLLAIHLKRDTWDGGMKLAPWVGAIARHKIIDALRRRGARQF
                     APIDDFEAVIPAPEGDDPHDLSDADRLIGQLPARQQDIVRSISLQGQSIAATAKRLSM
                     SEGAVRVALHRSLKALAAAWRRTAP"
BASE COUNT          851 a         1602 c         1631 g          790 t
ORIGIN      
        1 ccgcaggagg aaatgccgcc ctcgtcgctc gagaccctgc gcttcttcga aaaattcgcc
       61 gagcgccatt cggtcgatat cgagccgatc accaagcgcc aactcgccga gcttgccgaa
      121 ttcgacggcc tgttcatccg ggcgacgacc tcgatcgaca attacaccta tcgcttcgcc
      181 cgccgcgcct tgcaggaggg catgccggtc atcgacgatc cggtctcgat gatccgctgc
      241 accaacaagg tctatcttca tgagttgatg gaggcgaacg gcgtaccgat cccgccgacg
      301 atcatgctcg ccgaggaaga ggacctcgaa aaggccgcca aggcgctcgg ctggccgatg
      361 gtggtgaaaa tccccgacgg ctcgttttcg cgcggcgtgc accgggtcga aaatttcgag
      421 gctttgaaga cgctggctga ccagctcttc gaggataccg atctgctcct ggcgcagaaa
      481 ttcatgccga ccgatttcga ttggcgcgtc ggcgtgctcg atcgcgagcc gcttttcgtc
      541 tgccagtatc gcatggcgcg cggccattgg cagatcatca agcatggacc gaacggcgcg
      601 cgggagggcg gctcgaccac gatcgccctc aatgacgcgc cgccggaggt gatcgatgtc
      661 gccttgcgcg ccgcgagcgc catcggcgac ggcctctatg gcgtcgacgt caagcagacc
      721 gcgagcgaga tcgcggtgat cgaggtcaat gacaatccca atctcgacca tggctgcgaa
      781 gaccaggtcg gcaaggacga gatatggagc cgcatcctgc aatggttcgt gaagcggatc
      841 gacgcgtgag gctggcggcc ggacgacggc ggaatgggtc tcgacgtggg ggcctcctct
      901 ccccgtggag cgaagcggaa cggggagagg tacggagagg ggccggtcgt ctcgcttcga
      961 ccggcgcggc aaaccgacca gcccctctcc gtacctctcc tcgccttgcg gggagagggg
     1021 cttcgacgcc gcgtcttatc cttagccgcc agatcggcgc cggcttggct ttcaatgcgc
     1081 attggcgaac ccccctatga cgtctgtgac gttcgatcct cgcctgacgc cggcgcgccc
     1141 cgatctcgcg gcggcgttcc tgcgcggcaa ggtggaggcg gcgcgcttcg tcgaaggatt
     1201 gcgccgccgc gtcgccgagc cggtcgcgcc gctgcgccgc gcgccggcgc atgacgcgat
     1261 gctcgagact caggcgcttt atggcgagga ggttgccgtt ttcgaggaga acgagggctg
     1321 ggcctgggtg cagctccaac gcgattcgta cgtcggctat atgccggccg ccgcgctcgc
     1381 ggctgaagcg gcgccgacgc accgcgtcgt cgtcctgagc acgttcgtct atccgaagcc
     1441 gaacatcaaa gcgccgccgc tgatggcgct gccgctcgat gcgcggatcg agatcaaggc
     1501 cggcgaaggc gagttttgcg cgctggccgg aggcggcttc atctatgctc gccatgtcgc
     1561 gccgctcggc gcgttcgagc cggatttcgt cgcggtcgcc gagcgctttc tggaggcgcc
     1621 gtatctctgg ggcggccgta ccgcgcaggg actcgactgc tcggcgctgg cgcagaacgc
     1681 gctcgccgcc gccggccgtt ccgcgccgcg cgatacggat atgctggaag cggcgctcgg
     1741 ctcgcccgtc gctttcgatc aaagcctcgc cggcctcaaa cgcggcgatc tcgtcttctg
     1801 gaagggccat atcggcgtga tgcgcgacgc ggcgatgctt ctgcacgcca gcggctggca
     1861 tatgcaggtt gtcgccgagc cgctcgccgg cgcgcgcgac cgcatcgccg ccaaaggcgg
     1921 cggcccgatc acatccattc gacgattaag cgcagagtaa atcccttctc cgcttcgcgg
     1981 ggagaaggga ggtacggcct acttcttagc cttgcccatc gtcagattgc cgaagcgcga
     2041 attgaagcgc gacaggcggc cgccgcggtc gagcaattgc tgcgagccgc cagtccaggc
     2101 cggatgggtt ttcgagtcaa tatcgagatg gagcgtcgcg cccggctcgc cataggtcga
     2161 gcgggtcgtg aattccgtgc cgtcggtcat gacgaccttg atcatgtggt aattgggatg
     2221 gatatcggcc ttcatcgcct gtcctcggcc ttaataaaga gagccgcccg atgatcggcg
     2281 gctcgcgctt gagcggtcct ataatgcagg ctcgggcgcg agacaagcgc aggtcgccgg
     2341 gcgaacgcgt ccagccactc ggctctgccg ctaacatatt ggccgccaaa tcgaattcgc
     2401 cgaaaggagc gctcggtccg tcttgctccg agggcaaaaa aagtccaagg tcgttcggct
     2461 attcaggaga gatcaacgcg atgaactgga cctcggcgat gacgcggcgc gctgcggccg
     2521 ggcttgccgc gctcggcctg tggctggccg ccacggcgac gggggctcag accgccgcgc
     2581 ccaagccgga aggctttgcc gatatggcgc gggcgctcga accggggccc ttgcccgaat
     2641 tgtcggtcgg cgacaaagcg ggcgtgccga tgatcgaata cggctcggtc acctgcacgc
     2701 attgcgccca tttcgacaag gaggtctggc cggcgatcaa ggccgcctat gtcgataccg
     2761 gaaaggtccg ctatattttc cgggaatatt cgcgcaacag cctcgatgtc ggcgccttca
     2821 tgctggcgcg ctgcaacggc gacgacaagg cgctggcgac gatcgagctg ctctttgcga
     2881 cgcaggacca atgggctttc gtcgataagc cgctcgacgg cttggtcgac gtgctcaagc
     2941 aaaccggcct gacccgcgac aagatcgaga cttgtctgaa ggatcagggc aaagccgacg
     3001 cgctggtcaa tatcgtcaag acggcggacc agaatttcgc cgtcaaaggc acgccgacct
     3061 ttatcatcga cgggaaaatc tatggcggcg cgctgagcaa ggacgagctc gacgcgattc
     3121 taaagccgct ggtcaaatag ggcggcggaa tacgaggccg cggcgtcgaa gcccctctcc
     3181 ccgcgaagcg gggagaggta cggtgagggg cggggcggta agcctcgacg gtcgaggaaa
     3241 tccgctgtcc ggcgaaagcg cctcggacga acgccacacg accagcccct ctccgcacct
     3301 ctccacgttc cgcttcgctc cacggggaga ggtgtccccg gcgccgagcc cttggctcct
     3361 cgcaacgcgc cggctcacca ccgcaaaacg cgaccgccaa tgaaagcgcc cacggcgacg
     3421 acgatcatcg tcgccagcgt gtaccaggtc gcgacgaaga gcggcgaatc gtcggtgcaa
     3481 ttggtggcgt agagggtcgc ggcgattccg gccgagacga ggccggccgc cgctccggcg
     3541 agaggcggag aagccggcgc gccggcgcgc atggcgagga gaagcgccgc gagcggcgcg
     3601 atcgaaagga gcgggatcag cgtcaggcaa tggagggcgt tcgagccgat cagccttgcg
     3661 agccagagat cgggcggaac catcatcaat tccatcgcca cgctgccgcc gagcagaatg
     3721 agaggcgcgg caagcgcaat cgccagccag cggcgctcgg cgcccggccg caacaagcgc
     3781 caagtcgcga ggccggtcgg gatcgcgagg gcgatcgtat cgacgaattt ccaatcgaag
     3841 cgtatcgtgc cgagggcgcg cgcaatatcg tgccgcgggc cgaccatgcc caaaaagagg
     3901 caggcgctga tcagtgcgcc ggccgccagc gccaaaacga aacgccggct gagcggcggc
     3961 ccctgcggct tcggatcggc ggcgagcgcc gcgatgaggt cggcggtctt cacggcgcgg
     4021 tcctccgcca cgccgccgcc agcgctttca gcgaccggtg aagcgcgacc cgcaccgccc
     4081 cctcgctcat cgacaggcgc ttggcggtcg cggcgatcga ctggccctgc agcgagatcg
     4141 agcggacgat gtcttgctgg cgggcgggca attggccgat cagccggtcg gcgtcgctca
     4201 aatcgtgcgg atcgtcgccc tcgggcgcgg gaatgaccgc ctcgaaatcg tcgatcggcg
     4261 cgaattgccg cgcgccgcgc cggcgcaggg cgtcaatgat cttatggcgc gcgatcgcgc
     4321 cgacccaggg cgcgagcttc atgccgccgt cccatgtgtc gcgcttcaag tgaatggcca
     4381 gcaaagtctc ctgcaccacg tcctcgacat ccgcatcgcc cgagcgtgac cgcgcgaagg
     4441 ccccgcgcgc catggcgcgc accgagcggc cgatttcctc gagcagccgg cgataggtcg
     4501 cctcgtcgcc ggccagagcc gcgcgcatcc tgaccgccca gtcctcgtcg ctttccgctt
     4561 cgcccacggc ggcgttcgtc cttgcttcgc ggctgagagc caattgttac gccgctgctt
     4621 tgatttttat gtgaaatcgc gaatgcgccg ccggcacaag cgaaatctta cgacgcgctg
     4681 tggagaaggc gcgcccaagc gcggtcgcga atccccccgc cgcaaagcgg gagagagtga
     4741 ggggccgcgc ggagcctcga tgtgggcgtt tcgcagcgcg taccttcgag tcctgcaagc
     4801 ctctcatccg gcgcttcggg ccgcctcctc ccgcggagcg gcgagaacgg atttacttcc
     4861 cgccggccag cgcc
//
LOCUS       DATGJW010000345        16355 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1414711, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000345 DATGJW010000000
VERSION     DATGJW010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 16355)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16355)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16355
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1414711"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(108..338)
                     /locus_tag="VKU03_10500"
                     /pseudo
     CDS             complement(108..338)
                     /locus_tag="VKU03_10500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335689.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
     gene            complement(464..2788)
                     /locus_tag="VKU03_10505"
     CDS             complement(464..2788)
                     /locus_tag="VKU03_10505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018094500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLJ71740.1"
                     /translation="MARRAETRERTEAPLSIDDDAVRLREEVARLNRLVEEHAQERVS
                     LQALIDAVPDYLWVKDVDSAFVVANKAVLDDNRTPDRNSLIGLNDFALHPRERAQTFF
                     DAEQKVVRTGQAVVDLEEMIVNTEGATRWLLTTKTPLRDKSGEIIGVVGISRDITQRK
                     RDDALRAGEAQILEMIAMNAPLEDVLDRLMRVAEAQLEGVDCSTLLLDRSGTRLRDGA
                     GPSLDPAYRRLIDGTPIGPNVGSCGTAIFRRESVIVADIENDPLWAEYRDLVRPYGYR
                     SCWSTPILSHQGAALGAFAMYSKEARQPNAAELRMVAAATRLAGIAIERRQAEERIQF
                     MATHDALTGLPTRALLKDRLQQAMHFAERYDRWASLAFIEVDNFKQVNDSLGHGAGDE
                     LLKIVARRLTDCIRPTDTVARLGANEFAVVIADQPKSRERVNRSVQRLREAVAAPIAV
                     AGHHLQMTCSIGLANYPKDGGDVEAMLANAGSAMYRAKEMGRDNIQLYAPELNAKAHE
                     KFLLQEELRRALKRDEFTLLYQPQVDLRTGRIFAVEALIRWRHPELGLAPPDKFIPAA
                     EESGLIVPIGDWVLRAAARQNKAWQDAGLAPIVVSVNVSARQFREKNLVSRVVAALKE
                     SGLAAQYLELELTESLIMQDVEQAIATMKQLQGLGVQLSIDDFGTGYSSLSALKTFPV
                     ARLKIDKSFVRDLPDDENDRAVATAVISLGQKLNLRVIAEGVETDAQMAFLRDNNCDE
                     MQGYLFSKPAPPEEIAAMLKRPPRWPRGAQLLAS"
     gene            2932..4284
                     /gene="rimO"
                     /locus_tag="VKU03_10510"
     CDS             2932..4284
                     /gene="rimO"
                     /locus_tag="VKU03_10510"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HLJ71741.1"
                     /translation="MTTKTEAPPKAAPRISFVSLGCPKALVDSERIITRLRAEGYELA
                     RSHAGADAVIVNTCGFLDSAKQESLEAIGAAMAENGKVIVTGCMGAEPEPIRRSFPGI
                     SAITGPQAYESVMAAVREAAPGPHDPFLDLVPETGVKLTPRHYAYLKISEGCNNRCTF
                     CIIPKLRGDLHSRPAAEVLREAEKLVKAGVKELIVISQDTSAYGVDLRYAASAWRRDG
                     ETREVRAKFLDLARELGSLGAWIRLQYVYPYPHVDEVVALMAEGKVLPYIDIPFQHAA
                     PAVLKAMRRPGNQEKTLERIKAWRAICPDLTIRSTFIVGFPGESEEDFSFLLDWLDEA
                     RLDRVGAFKYEPVAGAAANDLGLPQVPAEVKEARWKRFMARQQAISAKRLAAKVGKRI
                     EAIVDVGGPKGGKGRSQGDAPQIDGVVHIAARRPLREGDIVTVKIERADAYDLHGVAA
                     "
     gene            complement(4291..5955)
                     /locus_tag="VKU03_10515"
     CDS             complement(4291..5955)
                     /locus_tag="VKU03_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006613827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HLJ71742.1"
                     /translation="MVEMTGAMAIVEQLVANGVDTVFGLPGAQIYSLFDALQQRADRI
                     RTYGARHEQACGYMAFGYARSTGRPGVFAVVPGPGVLNAAAALCTAYGCNAPVLCLTG
                     QVPSAFIGRRRGHLHELPDQLGVLSRLTKWAARIEQVEDAPDMVNEAFRRMLSGRPGP
                     VALEMAWDAMAAKAPVWRRRPATIAPPPAPDPAAVEAAAKLLVGARRPMIMAGSGAQH
                     AGAAVGALAEALDAPVAAFRGGRGVVGEDHPLGISSYAAYRLWPETDVLLGIGTRLEM
                     PYMRWTGMMTLIERPAPPPHLIRLDIDPDEMTRLIPHVGIVADAEAGAAALLEAVRRL
                     RAGPADGRAARAAIAEAKAAARAEFESVQPQIAYLDAIREVLPRNGIFVAELSQVGFA
                     SYFGYPVYEPRTYISEGHQGTLGFGFPSALGVKVAHPDRPVVAIAGDGGFMFAVQELA
                     TAAQERIGLITLVFNNRSYGNVLRDQRVGYGDRVIGARLDNPDFMTLAEAFGVQGRRV
                     GSPAALKEALERALKTVRPVLIEIEGEPGGEVSPWPFIHPKPAERV"
     gene            6332..6850
                     /locus_tag="VKU03_10520"
     CDS             6332..6850
                     /locus_tag="VKU03_10520"
                     /inference="COORDINATES: protein motif:HMM:NF013933.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family transporter"
                     /protein_id="HLJ71743.1"
                     /translation="MVLGACSADAFWAALVSLGVGAIFAAPAVRTILGVTSVILLLAL
                     AALFLRGAWRALARREQEAANPFRFDTGRAGFVLGATMALASPWNVAFWIAAVGRPEL
                     AGNGAYALLLMAAAVLSGALAWGLVWSGSVVLLRRRAGSKNSARWWTFGVELATALLM
                     IYFAVKSAVRIF"
     gene            complement(6847..7872)
                     /locus_tag="VKU03_10525"
     CDS             complement(6847..7872)
                     /locus_tag="VKU03_10525"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ71744.1"
                     /translation="MQSRAVGRASPSARRLAAVLFAVALTIPSAAWALDASQPPAPME
                     KIPLKLFKSAQQALRMGIEELRAGDSKSSVEALKYAAEGGQSLAQWKLGRMYADGDGV
                     PQDDLKAYEYFDEIVRNYSEDEQDRRDLGAVSNAIVAIGIYSLNGIANSSIQPDPERA
                     FQLFMYAATNFGDPDAQYNLARMYMDGTGGIEKNTMSAARWLALAAEKHHHPAQAVLG
                     HLLFVGDGLPRQRARGLMWLTIAKNAARGAKDQWMIDLYNKDFAAAGDEDKQMAALYL
                     GRYAKGREAPSPPPAEARLPASGDRLPTTPAMALAPPPPRVTPAAATPLADAGSPAGA
                     REDGSSR"
     gene            complement(8131..11019)
                     /locus_tag="VKU03_10530"
     CDS             complement(8131..11019)
                     /locus_tag="VKU03_10530"
                     /EC_number="6.1.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116205.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004832 - valine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006438 - valyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HLJ71745.1"
                     /translation="MEKTFDPAAVEGRISARWEAAGAFRAGRPERAEAEPFCIVIPPP
                     NVTGALHMGHALNNTLQDILCRYWRMKGRDVLWQPGTDHAGIATQMVVERQLMERQEP
                     SRRELGREEFLKRVWAWKAESGGAIVSQLKRLGASCDWSRERFTMDEGLSKAVVKVFV
                     QLYREGLIYKDKRLVNWDPALQTAISDLEVVQVECKGSFKWSRVDGAPLEPAALAKIL
                     GKNPNGHLYYFDYPIVDDDGAATGEVVTVATTRPETMLGDTGVAVHPDDARYKHLHGK
                     KVRLPLVGRLITIVADDYSDPEKGTGAVKITPAHDFNDFEVGKRHEGEGVRQVNIFDA
                     AARIAIRGAPAFLEGVPPQDKEHLATIDAVDGEDRFLARQRVVVMMAERGLLAKIEPH
                     AHMVPHGDRSNAVIEPWLTDQWYVDAKTLAQPALTAVRGGETLFTPKNWEKTYFDWLE
                     NIQPWCVSRQLWWGHQIPAWYGLDGAVFVAETEAEAVGAALADAVEKRLISDEEAKAL
                     TDDPDRLSTLVKRDEDVLDTWFSSALWPFSTLGWPEETPELARFYPNNVLVTGFDIIF
                     FWVARMMMMGLHFRRQIPFRDVYIHALVRDEKGAKMSKSKGNVIDPLDLIDRYGADAL
                     RFTLAAMAAQGRDIKLSESRIEGYRNFATKLWNAARFAEMNGCVRRADFDSKAARDPL
                     NRWILGEAGKAAAETGAAIEAFRFNDAANAVYRFVWNIFCDWHLELVKPVLQGPDGPA
                     KEEARAVLAYVLDAAIGLLHPFMPFLTEELWAVKAGGAPTDLLALGPWPMAEGVADAA
                     AEAEIGWVIDLVSEIRSARSEMGVPAGAQVPLELVKASPEVRAYARRWEEPIKRLARL
                     HTIGFADAVQPGSAQLFVRGGIAVLPLQGLIDFSAERARLSKERDTQRKEIERIDQKL
                     ANPDFVKRAPEEVVEENRERRETASDRLGKIEAALQRIDAALARATPA"
     gene            complement(11199..11765)
                     /locus_tag="VKU03_10535"
     CDS             complement(11199..11765)
                     /locus_tag="VKU03_10535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2497 domain-containing protein"
                     /protein_id="HLJ71746.1"
                     /translation="MSANPSYDLDALHRAQRAYEPSMEEILASIRNIISEERPGLAPA
                     ARPAPVAEPEPPAPPAAERREPVERAEPAAMPRVVWPRPEAEAPLETQPAVAAVAPPP
                     IPDQASLPEVADDPQDRLISPATDAAIGHSFDALSVSVAVQNSNVIEETVRDMIRPML
                     KAWLDDHLPTMVENLVRAEIQRVARGGR"
     gene            complement(12018..13433)
                     /locus_tag="VKU03_10540"
     CDS             complement(12018..13433)
                     /locus_tag="VKU03_10540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01844.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009279 - cell outer membrane [Evidence
                     IEA];
                     GO_function: GO:0015288 - porin activity [Evidence IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TolC family outer membrane protein"
                     /protein_id="HLJ71747.1"
                     /translation="MAVGQLFRAARDYRRLAARGAVFATIAAFTASPAEAETLAGALA
                     KAYLNNPDINQQRAGVRASDENVPKANAGFLPTINGQASYGFSDTQFSAPGQSGTLIA
                     HPRGFGVTATENLWNGNRTINGVRQAESSVLASREQLRNTEQNVLLNAVTFYMNVLRD
                     SAILSLNRANVDVLKEQLRNTQDRFNVGEVTKTDVAQAQASLAGAEAQALSAQSTLQS
                     SIASYRQTVGDQPKSLAPVKPLERLLPRSLQEAIAIGLVEHPAIVAALHGVDAAELNV
                     KIVEGALLPTLSAQLQVADQWDSAAVATIPNHVINATATAQLTIPIYQGGSEYASTRQ
                     AKEQLTQQELQVAQQRDAVRAAVTSAWWLYSVSPAVIRSSKAQVDAASVALAGVREEA
                     KVGQRTTLDVLNAQQALLNARVQLVSAEHDQVVASYALLSAIGRLSTANLGVAVAQYD
                     PRVHFDQVKDKWIGLRTPDGK"
     gene            complement(13695..14363)
                     /locus_tag="VKU03_10545"
     CDS             complement(13695..14363)
                     /locus_tag="VKU03_10545"
                     /inference="COORDINATES: protein motif:HMM:NF013315.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate O-methyltransferase"
                     /protein_id="HLJ71748.1"
                     /translation="MNRPTSPEIYGDRRWRMIENQLRPSGVNDLPLLAAFAETPREAF
                     VEPAFASLAYLDRDVPALQGAGRLLLAPASLGRLLQAAKPQPGERILDVAGGSGYGAA
                     ILSRLGARVTALETAASGQGANLLLAGRPGVDVVAGELPAGLPAKAPFDAIVVNGAFE
                     VWPEQLIAQLAEGGRLVGFDATFPAPKGVLIEKAGGAASRRALFDAAAPRLDAFRRPA
                     QFAF"
     gene            complement(14415..14488)
                     /locus_tag="VKU03_10550"
     tRNA            complement(14415..14488)
                     /locus_tag="VKU03_10550"
                     /product="tRNA-Cys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(14454..14456),aa:Cys,seq:gca)
     gene            complement(14744..15466)
                     /locus_tag="VKU03_10555"
     CDS             complement(14744..15466)
                     /locus_tag="VKU03_10555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013166512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylcholine synthase"
                     /protein_id="HLJ71749.1"
                     /translation="MSVVRDKNRVPVRRAAAFSVHVLTASGGAVAVLALYAAIERDFT
                     AVFAWLGLAIFIDGIDGTLARAAHVAEVAATINGVVLDLVVDFLSYVLVPVVAIWRSD
                     LMPTPIAFWIGLAVIIASALYFADTRMKTADNWFRGFPALWNVLAFYLFVFRLPSLVN
                     AAILVAAAGLMFAPVVFVHPMRVEKMRAATLAATLLWGVCAVIAVWKDLAPGFWVKTG
                     LAAAAVYFIALPLQRHSFWAEE"
     gene            15548..>16355
                     /locus_tag="VKU03_10560"
     CDS             15548..>16355
                     /locus_tag="VKU03_10560"
                     /inference="COORDINATES: protein motif:HMM:NF013646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HLJ71750.1"
                     /translation="MLDSQRQPAITAEILVAGAGLAGLAAAIAFAKAGFETVLCGSPD
                     RAAPGRTVALLDGSMRFFERLGLRAEIERLGAPLKALRLIDDTDSLWRGPVVEFHAEE
                     IGLEAFGWNIENSALTDALWAAAGRLPGLTAIASRIARYEWGATRALTQCQDGRLVAA
                     RLVAAADGRDSPARLSADIPIRARPLAQTALTVFLGHTRPHRDFSTEFHTRQGPFTLV
                     PLPPAGESAHRSSLVWVMTRQQAARRMALADDALAAEIEAQASSIHGAMRI"
BASE COUNT         2769 a         5445 c         5368 g         2773 t
ORIGIN      
        1 tgcgaaaacg cggccccctg cggcgtggcg caaacaagcc ttggactctc tgacaaggca
       61 cggaggcctg ccgcggctct cgcgttcaca tcgcgcgcag caactcccgg cgcagccggc
      121 tcggacaaag cccgcataaa agctttaaaa ccggcgggac tcattcggat cggccgcgtt
      181 tccatcaaca catcctcagc cgcgcgcacc gcggcatcgc gcacgaaatc cgtgcgcgag
      241 cggccacgca acgttgcggc tcgatcgatg agagcgatat cggcttccgg caatcgcatt
      301 gagagcggat gctccttgcg tgcggctgtt ttgggcattg aggcctccaa cgacaattta
      361 gaggcgttgt agcgtatttt gtggaggcgc aagcggattt tcgcctcggc gtcgaggcaa
      421 acggcccgat tgcctgaaaa aaatgttgta tttgtacttc gcgctagctc gccagaagct
      481 gcgcgccgcg cggccagcgc ggcgggcgct tcagcatcgc ggcgatctct tcaggcggcg
      541 cgggcttgga gaaaagatag ccctgcatct cgtcgcaatt gttgtcgcga aggaacgcca
      601 tctgggcgtc ggtctcgacg ccctcggcga tcacccgcaa gttcagcttc tggccgagcg
      661 agatgaccgc ggtcgccacc gcccgatcgt tctcgtcgtc cggcagatcg cggacgaagc
      721 tcttgtcgat cttgagccgg gcgacgggaa aggtcttcaa ggcgctcaag ctcgaatagc
      781 cggtgccgaa atcgtcgatc gagagctgca ccccgagacc ctgcaattgc ttcatcgtcg
      841 cgatcgcctg ctcgacatcc tgcatgatca ggctttcggt caattccaat tcgagatatt
      901 gcgccgccag cccgctctcc ttcagcgccg cgaccacccg gctgaccaaa ttcttctccc
      961 tgaactgacg cgccgagaca ttgaccgaga cgacgatcgg cgcgaggccg gcgtcctgcc
     1021 aggccttgtt ctgcctggcg gcggcgcgaa gaacccagtc gccgatcggc acgatcagcc
     1081 cgctctcctc ggcggcggga atgaatttgt ccggcggcgc caatccgagc tcgggatggc
     1141 gccagcggat caaggcctcg acggcgaaga tgcggcccgt gcgcaggtca acttgcggct
     1201 gatagagcag cgtgaactcg tcgcgcttga gcgctctgcg caactcctct tgcaacagga
     1261 atttctcatg cgccttggcg ttgagctcgg gcgcatagag ctggatgttg tcccgcccca
     1321 tctccttggc gcggtacatc gcggagccgg cattggcgag catcgcctcg acgtcgccgc
     1381 cgtccttggg atagttggcg aggccgatcg agcacgtcat ctgcagatga tggccggcga
     1441 cggcgatcgg cgcggcgacc gcttcgcgca agcgctggac gctacgattg acgcgttccc
     1501 tgcttttcgg ctggtcggcg atgacgacgg cgaattcgtt cgcgccaagg cgcgccaccg
     1561 tatcggtcgg gcgaatgcaa tcggtcagcc ggcgggcgac gatcttgagc aattcgtcgc
     1621 cggcgccgtg gccgaggctg tcgttgacct gcttgaaatt gtcgacctcg atgaaggcga
     1681 ggctcgccca ccgatcgtag cgctcggcga aatgcatggc ctgctggagg cggtccttta
     1741 gcagcgcgcg ggtcggcaag ccggtcagcg cgtcatgggt ggccatgaat tggatgcgct
     1801 cctcggcctg gcggcgctcg atggcgatgc cggcgagccg cgtcgccgcc gcgaccatgc
     1861 gcaattcggc ggcgttgggc tggcgcgcct ctttcgaata catcgcgaaa gcgccgagcg
     1921 ccgcgccctg atgagaaagg atcggagtcg accagcacga gcggtagcca tagggccgga
     1981 ccaaatcgcg atactcggcc cagagcggat cgttctcgat atcggcgacg atcacgcttt
     2041 cgcggcgaaa gatcgccgtg ccgcaggagc cgacattcgg gccgatcggc gtgccgtcga
     2101 tcaggcggcg ataggccggg tcgaggctcg gcccggcgcc gtcgcgcagc cgcgtccccg
     2161 accggtcaag caacagcgtc gagcaatcga cgccttcgag ctgcgcctcg gcgacccgca
     2221 tcagccgatc gagcacgtct tcgagcggcg cgttcatcgc gatcatttcg agaatctggg
     2281 cctcgccggc gcgcagcgcg tcgtcccgct tgcgctgggt gatgtcgcgc gaaatgccga
     2341 ccacgccgat gatctcgccg ctcttgtcgc gcaacggcgt cttggtcgtc aaaagccagc
     2401 gcgtcgcccc ctcggtgttg acgatcattt cttcgagatc gaccaccgcc tggccggtcc
     2461 gcaccacctt ctgctcggcg tcgaagaacg tctgcgcgcg ctcgcgcgga tggagcgcga
     2521 aatcgttcag cccgataagg ctgttgcggt ctggcgtccg gttgtcgtcg agaaccgcct
     2581 tgttggcgac gacgaaagcg ctgtcgacat ccttgaccca gagataatcg ggcaccgcgt
     2641 cgatcaatgc ttgcagggag acgcgctctt gcgcgtgctc ctcgacgagg cgattgaggc
     2701 gcgcgacctc ttcgcgcagc cggacggcgt cgtcgtcgat tgaaaggggc gcttccgtcc
     2761 gctccctcgt ctcggcgcgg cgcgccatcc gtgtcctaaa cccgctcatc gcatgaccag
     2821 atgcggaaat tcatccggcg gcgaccttaa ggtttcaaca ttaacaagct cttgcggttc
     2881 gcgcggagcg acgggttgcg ctcttggacg ccgcgtggca agcgaccggc catgaccacc
     2941 aagacagagg ctccgccgaa agcggcgccg cgcatttcct ttgtctcgct cggctgtccc
     3001 aaggcgctgg tcgacagcga gcggatcatc acgcgattgc gcgccgaggg ttacgaactc
     3061 gcgcgcagcc acgccggcgc ggacgcggtg atcgtcaaca cttgcggctt tctcgatagc
     3121 gccaagcagg aatcgctcga ggcgatcggc gccgccatgg ccgagaatgg caaggtgatc
     3181 gtcaccgggt gcatgggcgc ggagccggag ccgatccggc gaagcttccc gggaatcagc
     3241 gcgatcaccg gcccgcaggc ctatgaaagc gtcatggcgg cggtgcggga ggccgccccg
     3301 gggccgcatg atccatttct cgacttggtt ccggagaccg gcgtcaagct gacgccgcgc
     3361 cactatgctt atttaaaaat atcagagggc tgcaacaatc gttgcacgtt ttgcatcatc
     3421 ccgaagctgc gcggcgatct ccattcccgg ccggcggccg aggtgttgcg cgaggctgag
     3481 aagctggtca aagccggcgt caaggagctg atcgtcatct cgcaggacac cagcgcctat
     3541 ggcgtcgatc tgcgctacgc ggcgagcgcg tggcggcgcg acggcgagac gcgcgaggtg
     3601 cgcgccaaat tccttgatct tgcgcgcgag cttggctcgc ttggcgcttg gattcgcttg
     3661 caatatgtct atccctatcc gcatgttgac gaggtcgtcg cgctgatggc ggagggcaag
     3721 gtcctgccct atatcgacat tccgttccaa cacgccgcgc ccgcggtgct gaaggcgatg
     3781 cgccggccgg gcaatcagga aaagacgctg gagcggatca aggcttggcg cgccatctgt
     3841 cccgatctga ccattcgctc gactttcatc gtcggctttc ccggcgagag cgaggaggat
     3901 ttctcgttcc tgctcgactg gctcgacgag gccaggctcg accgggtcgg cgccttcaaa
     3961 tacgagccgg tggccggcgc ggcggccaac gatctcggcc tgccgcaggt ccccgccgag
     4021 gtcaaggagg cgcgctggaa gcgcttcatg gcgcgccagc aggcgatcag cgccaagcgt
     4081 ctcgccgcca aggtgggcaa gcggatcgag gcgatcgtcg atgtcggcgg ccccaaaggc
     4141 ggcaagggcc gctcgcaggg cgacgcgccg caaatcgacg gggtcgtgca tatcgccgcg
     4201 cgccggccct tgcgcgaagg cgacatcgtg acggtcaaga tcgagcgcgc cgacgcctac
     4261 gatctacacg gcgtcgcggc ttgaagagcg ttaaacccgc tcggccggtt tcgggtggat
     4321 gaaaggccag ggcgaaacct ccccgcctgg ctccccttcg atttcgataa ggaccggacg
     4381 gaccgtcttc agcgcgcgct ccagcgcttc cttcagggcc gccggcgagc cgacgcggcg
     4441 cccctgcacg ccgaacgctt cggcgagcgt catgaaatcc ggattgtcca gcctcgcgcc
     4501 gatcacccgg tcgccatagc cgacgcgctg gtcgcgcaag acattgccat agctgcgatt
     4561 gttgaagacc agcgtgatca gaccgatgcg ctcctgcgcc gcggtggcca attcctggac
     4621 cgcgaacatg aagccgccat cgccagcgat cgccacgacc gggcggtcgg gatgggcgac
     4681 cttgacgccc aaagcggaag ggaagccgaa gcccagcgtg ccttgatggc cctccgatat
     4741 gtacgttcga ggctcataga ccgggtagcc gaaatacgag gcgaagccga cttgcgagag
     4801 ctcggcgacg aagatcccgt tgcgcggcag gacttcgcga atggcgtcga gataggcgat
     4861 ctgcggctgc acgctttcga attccgcccg cgccgccgct ttcgcttcgg caatggcggc
     4921 gcgcgcggcg cggccgtcgg ccggcccggc gcgcaagcgc cgcaccgctt cgagcagcgc
     4981 cgccgcgccg gcctcggcgt cggcgacgat cccgacatgc gggatgagcc gggtcatttc
     5041 gtccgggtcg atgtcgaggc ggatgagatg gggcggcggc gccggccgct cgatcagcgt
     5101 catcatgccg gtccagcgca tatagggcat ttccagccgg gttccgatcc cgagcagaac
     5161 gtcggtttcc ggccagaggc ggtaggcggc gtaggaggag attccgagcg gatgatcctc
     5221 gccgaccacg ccgcggccgc cgcggaaagc ggcgaccggc gcgtcgagcg cctcggcaag
     5281 cgcgccgacg gcggcgccgg cgtgctgcgc gccgcttccg gccatgatca tcggccggcg
     5341 cgcgccgacc aggagcttcg ccgccgcttc gacggccgcc ggatcgggcg cgggcggcgg
     5401 cgcgatcgtt gcgggccggc gacgccagac cggcgctttg gcggccatcg cgtcccaggc
     5461 catttccaga gcgaccggcc ccggccggcc gctgagcatg cgccggaagg cttcgttgac
     5521 catgtcgggc gcgtcctcga cctgctcgat ccgcgccgcc catttcgtca gccggctgag
     5581 cacgccgagt tggtcgggaa gctcatgcaa atggcctcgc ctccggccaa tgaacgcgct
     5641 cgggacctgg ccggtcaggc aaaggaccgg cgcgttgcag ccataggccg tgcacaaagc
     5701 ggcggcggcg ttcaacacgc ccggcccggg cacgaccgcg aagacgcccg gccgaccggt
     5761 cgagcgggca tagccgaagg ccatgtagcc gcaagcctgc tcatgccggg cgccataggt
     5821 cctgatccgg tcggcgcgct gctggagcgc gtcgaaaagc gaataaatct gcgcgcccgg
     5881 aagaccgaag accgtgtcga cgccattggc gacgagctgc tcgacgatcg ccatggcgcc
     5941 ggtcatttcg accaacgcga acgcttcgga cattctgcga aatcccaagg acccgccgag
     6001 cttatcgccg cccgcccggc gcagcgagcg ataatcgccg aagagcgatc agacggagat
     6061 cgcccctcga cctttccgcg ccggcgcggc attgtttcga ggtctattcg gtctctcggc
     6121 aaatcgccgc gcttgcggcg cagaccgccc gccctggccg gggatcgctc caatctcgaa
     6181 agccggcgac cgccccgcta tgctcgctcc tgtttttcct ttgctcgcga ccggctttct
     6241 cctcggatgg tcggtcgcct ggccgcccgg gccaatcaac tcggaaatcg cgcgccgctg
     6301 cctcgccggc ggattctagg ccggcttcac cgtggttctc ggcgcgtgca gcgccgacgc
     6361 tttctgggcg gcgctggttt cgctcggcgt cggagcgatc ttcgccgcgc cggccgtgcg
     6421 caccatcctc ggcgtgacca gcgttatctt gctcctcgcc ctggcggcgc tctttttgcg
     6481 cggcgcctgg cgcgcgcttg cccgccgcga acaagaagcg gcgaatccct tccgcttcga
     6541 taccggccgc gccgggttcg tgttgggggc gaccatggcc ttggcgagcc cgtggaacgt
     6601 cgccttctgg atcgccgccg tcggccggcc ggaactggcc ggcaacggcg cctacgccct
     6661 gttgctgatg gcggcggccg tcctttcagg cgcgctcgcc tggggcttgg tttggtcggg
     6721 atcggtcgtc cttcttcgcc gccgagccgg ctcgaagaac agcgcgcggt ggtggacctt
     6781 cggcgtcgaa ctcgccacgg ccctgctcat gatctatttc gccgtgaaat cggcggtccg
     6841 gatcttttag cgcgacgaac cgtcttcacg cgcgccggcc ggcgatccgg cgtcggcgag
     6901 cggcgtcgcc gcggcgggcg tgacgcgggg gggcggcggc gcgagcgcca tggccggcgt
     6961 cgtcggcaga cggtctcccg aggcgggcag gcgcgcttcg gcaggcggcg gagacggcgc
     7021 ctcgcgcccc ttggcgtatc ggccgagata aagcgccgcc atctgcttgt cttcgtcgcc
     7081 ggccgccgcg aaatccttgt tgtaaaggtc gatcatccac tggtctttag cgccccgggc
     7141 ggcgttcttg gcgatcgtca gccacatcag gccgcgcgcc cgctggcggg gcaaaccgtc
     7201 gccaacgaag agcagatgcc caagcaccgc ctgggccggg tggtgatgct tctccgcggc
     7261 cagcgccagc cagcgcgcgg cgctcatcgt gttcttctcg atgccgccgg tcccgtccat
     7321 atacatccgg gcgagattgt actgggcgtc gggatcgccg aaattcgtcg ccgcatacat
     7381 gaacaattga aaagcccgct ccggatcggg ctggatcgac gaattggcga tgccgttgag
     7441 gctgtaaatt ccgatcgcga cgatcgcgtt ggagaccgcg ccgagatcgc gccggtcctg
     7501 ctcgtcctcg ctataatttc ggacgatctc gtcgaaatac tcgtacgcct tcaaatcgtc
     7561 ctgcggcacg ccgtcgccat cggcgtacat gcgcccgagc ttccattgcg ccagggactg
     7621 gccgccctcg gcggcgtatt tgagcgcctc gaccgacgac ttcgaatcgc cggcgcggag
     7681 ctcctcgatg cccatccgga gcgcttgctg agcgctcttg aacagtttga gcggaatttt
     7741 ctccatcggc gccggcggct gggaagcgtc gagagcccaa gcggcgctgg gtatggttaa
     7801 cgctacggcg aagaggacgg cggcgaggcg gcgcgccgag ggcgacgctc ggccaacggc
     7861 gcgggactgc atgtctctgc agcctctatt cccgccgccg atgtccaacg cgcccatact
     7921 agctccaact ggtcgactct ttagcggtcg aggccatcat cagaagcggg aaagatgacg
     7981 acaatccgat agtttgcgtc acctcgcacg atccgaatta gcgcgtggtt tatggcgcaa
     8041 tcgcggcgcg ttaaagcagg aaatcaggct gttttcactt ggcgggggga tgttgctcaa
     8101 ttgcaacgaa tcccttcgta acaggcgttt ttaggccggc gtcgccctcg ccagcgccgc
     8161 gtcgatccgt tgcaaagccg cttcgatctt gccgagccga tcgcttgcgg tctcgcgtcg
     8221 ttcccggttt tcctcgacca cttcctccgg cgcccgtttg acgaaatccg gattggcgag
     8281 cttctgatcg attcgctcga tctcctttcg ctgcgtatcg cgctccttgc tcaggcgggc
     8341 gcgctcggcc gagaaatcga taaggccttg cagcggcaaa acggcgattc cgccgcggac
     8401 gaacaactga gccgagcctg gctgaaccgc gtcggcgaag ccaatcgtat gcaggcgcgc
     8461 caagcgcttg atcggctctt cccagcggcg ggcgtaagct ctcacctcgg gactggcttt
     8521 gaccagttcg agcggcacct gtgcgccggc cggcacgccc atctcggagc gcgccgagcg
     8581 tatctcggag acgagatcga tcacccagcc gatttccgct tcggccgcgg cgtcggcgac
     8641 gccttccgcc atgggccagg gaccaagcgc cagaaggtcg gtgggcgcgc cgccggcttt
     8701 gaccgcccat agctcttcgg tgaggaaggg catgaacgga tggagcaggc cgatcgccgc
     8761 atcgagcaca taggcgagga ccgcccgcgc ctcctccttc gccggcccgt ccggcccttg
     8821 cagcacaggc ttgaccagtt cgagatgcca gtcgcagaag atattccaga cgaagcgata
     8881 gacggcgttg gcggcgtcgt tgaaccgaaa ggcctcgatc gccgcgccgg tctcggctgc
     8941 cgccttgccg gcctcgccca aaatccagcg gttgagcgga tcccgcgccg ctttcgaatc
     9001 gaaatcggcg cgccggacgc agccgttcat ctcggcgaaa cgcgcggcgt tccaaagctt
     9061 ggtcgcgaaa ttgcggtagc cctcgatgcg gctctcggaa agcttgatat cgcggccctg
     9121 agcggccatc gccgcgaggg tgaagcgcaa agcgtcggcg ccgtagcggt cgatgaggtc
     9181 gagcgggtcg atgacattgc ccttcgactt tgacattttc gcgcccttct cgtcgcggac
     9241 cagggcgtgg atatagacgt cgcggaacgg aatttgccgg cggaaatgca ggcccatcat
     9301 catcatccgc gcgacccaga agaagatgat gtcgaagccg gtgacaagga cgttgttcgg
     9361 gtagaagcgc gcgagctccg gcgtctcctc cggccagccg agcgtcgaga acggccacag
     9421 cgccgacgag aaccaggtgt cgaggacatc ctcgtcgcgc ttgaccaagg tcgatagccg
     9481 atccggatcg tcggtcagcg ccttcgcctc ctcgtcgctg atcagtcgct tctcgacggc
     9541 gtcggcaagc gcggcgccaa ccgcctcggc ctccgtttcg gcgacgaaaa ccgcgccgtc
     9601 gagaccgtac caagccggaa tctggtgccc ccaccagagc tggcgcgaga cgcaccaggg
     9661 ctgaatgttc tccagccagt cgaaataggt tttctcccag tttttcggcg tgaacagcgt
     9721 ctcgccgccc ctcaccgctg tgagcgccgg ctgggccaag gtcttggcgt cgacgtacca
     9781 ttgatcggtg agccagggct caatgacggc gttcgagcgg tcgccatgcg ggaccatatg
     9841 ggcgtgcggc tcgatcttgg cgagcagacc gcgctcggcc atcatgacga cgacgcgctg
     9901 acgggcgagg aatcgatcct cgccgtcgac cgcgtcgatc gtcgccagat gctccttgtc
     9961 ctgcggcgga acgccttcaa ggaaagccgg cgcgccgcga atggcgatgc gggccgcggc
    10021 gtcgaaaata ttgacctggc gaacgccctc gccttcatgg cgcttgccga cttcgaaatc
    10081 gttgaaatca tgcgccggcg tgattttcac cgcgccggtg cctttttctg gatcggaata
    10141 atcgtcggcg acgatcgtga tcagtcgccc aaccagcggc aagcggacct ttttgccgtg
    10201 caaatgcttg tagcgcgcgt cgtccggatg aacggcgact ccggtatcgc cgagcatcgt
    10261 ctcgggacgg gtcgtggcca ccgtcacgac ctcgccggtc gcggcgccgt cgtcatcgac
    10321 gatcggatag tcgaaatagt acagatgccc gttcggattc ttgccgagaa tcttggccaa
    10381 agccgccggt tcgagcggcg cgccgtcgac gcgcgaccat ttgaacgagc ccttgcattc
    10441 gacctggacg acttccagat cggaaatagc ggtctgcagc gccgggtccc aattgacgag
    10501 ccgcttgtcc ttgtagatca gcccttcccg gtagagctgg acgaagactt tgaccaccgc
    10561 cttggacagg ccctcgtcca tcgtgaagcg ctcgcggctc caatcgcagg aggcgccgag
    10621 gcgcttgagc tggctgacga tcgcgccgcc ggattcggct ttccaggccc agacgcgctt
    10681 caggaattcc tcgcggccga gttcgcgccg gctgggctcc tggcgctcca tcaattggcg
    10741 ttcgacgacc atctgggtgg cgatgccggc gtgatcggtg cccggctgcc aaagaacgtc
    10801 cctgcccttc atgcgccaat agcggcagag aatatcctgc agcgtattgt tgagcgcatg
    10861 gcccatatgc agggcgccgg tgacattggg cggcgggatg acgatgcaaa agggctccgc
    10921 ctcggcgcgc tccggccggc cggcgcggaa ggcgcccgcc gcctcccatc gcgccgaaat
    10981 gcgaccctcg acggcggcgg ggtcgaacgt cttctccatc atgccaaaat ccaacacttc
    11041 tccggcgggc gcggcagcct cgcgaggcca aaaaaagcgc gatgcgcagg agcgaggctg
    11101 gcgcggcgca gcaaagactg cggcgaaggc gcttaaagcc ttagcggcgc ggcaaagtca
    11161 aacgccgcgc caaaagcagg gtcggaaggg atgaggcgtc agcggccgcc gcgcgccacg
    11221 cgctgaattt cggcgcggac gagattttcg accatggtcg ggaggtgatc gtccagccac
    11281 gccttgagca tcggccggat catgtcgcgc accgtctctt cgatcacgtt cgaattctgc
    11341 acggcgacgc tgaccgagag cgcgtcgaag gaatggccga tcgctgcgtc cgtcgccggc
    11401 gaaatcagcc gatcctgcgg atcgtcggcg acttcaggaa ggctggcctg gtccggaatg
    11461 ggcggcggcg cgaccgccgc aacggccggc tgcgtttcga gcggcgcctc ggcctcgggc
    11521 ctcggccaaa cgactctcgg catcgcggcc ggctcggccc gttcgaccgg ctcgcgccgc
    11581 tcggcggctg gaggcgcggg aggctccggc tcggcgaccg gcgccgggcg cgctgcgggc
    11641 gcgaggccgg ggcgctcttc cgagatgatg ttgcggatcg acgcgaggat ttcttccatc
    11701 gacggctcgt aggcgcgctg ggcgcggtgc aaagcgtcga gatcgtagga tggattggcg
    11761 ctcatgcccg tttcatacct tttggcgcgc gattgacgcc gcctctccag gtcccgcccg
    11821 ccctacggac cagtatcgag cttagcgacg tgaatgaaat tcctagctga gtcgccgggg
    11881 aatcgtctac cggcttggcg gaggcgaagc cgggttgtcc cgggaaatga tgtcgcggcc
    11941 gcgcgcccaa atgcggccct cgcagatctc tcgcgcccag cgccgcggcc ttcttcagac
    12001 ctggcgagat cggggcgcta cttgccgtcc ggcgttcgca agccgatcca cttgtccttg
    12061 acctggtcga aatggaccct cgggtcgtat tgagcgacgg cgacgccgag attggcggtc
    12121 gacagccggc cgatcgccga aagcagcgca taggacgcaa cgacctgatc atgctcggcc
    12181 gacaccagct ggactcgcgc attgagcaag gcttgctggg cgttgagcac gtccagcgtc
    12241 gtgcgctggc cgacctttgc ctcttcccgc acgccggcca gcgccaccga cgccgcgtcg
    12301 acctgcgcct tcgaagagcg aatcaccgct ggcgaaaccg agtagagcca ccatgccgag
    12361 gtcaccgcgg cccgaaccgc gtcacgctgc tgcgcgacct gcaattcctg ctgcgtgagc
    12421 tgctccttcg cctggcgggt tgaggcgtat tccgaaccgc cctgataaat cgggatcgtc
    12481 agttgcgcgg tcgcggtggc gttgatgacg tgattcggaa tggtggcgac cgcggcggag
    12541 tcccattgat cggcgacttg cagttgcgcg ctcaaagtcg gcagcaacgc cccctcgacg
    12601 atcttgacgt tcaattcagc cgcgtcgacg ccgtgcaggg ccgcgacgat ggcgggatgc
    12661 tcgaccagcc cgatcgcgat cgcctcctgc agcgatctcg gcaagagtct ttcgagcggc
    12721 tttaccggcg cgaggctttt cggctggtcg ccgaccgtct ggcgatagct cgcaatcgag
    12781 gattgcagcg tcgattgcgc gctcagggct tgcgcctcgg ccccggccag actcgcttgg
    12841 gcctgggcca cgtcggtctt ggtcacttcg ccgacattga agcggtcctg ggtgttgcga
    12901 agctgctcct tgagcacgtc gacattggcg cggttgaggg aaagaatcgc cgaatcgcgc
    12961 aacacgttca tgtaaaacgt gaccgcgttg agcaggacgt tctgctcggt gttgcgcaat
    13021 tgctcccgcg acgccagcac cgaggactcg gcctggcgca cgccgttgat ggtgcgattg
    13081 ccgttccaga ggttttccgt ggcggttacg ccaaagcctc gcggatgggc gatgagcgtg
    13141 ccggactggc ccggcgcgct gaattgagtg tcggaaaaac cgtagctcgc ctggccgtta
    13201 atggtcggca aaaagccggc attggccttc ggaacgttct catcgctcgc ccggacgcct
    13261 gcccgctgct ggttaatgtc cggattgttg agataagcct tggccagcgc ccccgccaaa
    13321 gtctctgcct ccgctggcga agcggtaaaa gctgcaatcg tcgcgaagac cgcgcctcgc
    13381 gccgcgagac gccgatagtc gcgcgccgcg cggaacaact gccccactgc catcaatacc
    13441 ccgtcttatt ccctatccgg ccgcgctttt tagatcgcgt gcggagcgtc aatctgaacg
    13501 ccttgacgcc gccgcaaccc gacgacgcaa ctgcgctacg caaacgcggc ctgacccggg
    13561 gccgaacacc aggaacctac gacgcaaaca taaaagatta ttcccctcta gccacccctt
    13621 tcgagcggct tggcgcccct ccaatccaca aatctccaca ggcgtcgcaa atatgcgaca
    13681 ctcgatgtca ggctctaaaa cgcgaattgg gccggccggc ggaaggcgtc gagccggggg
    13741 gccgccgcgt cgaacagcgc ccggcggctc gccgcgccgc cggctttctc gatcaggacg
    13801 cccttcgggg cggggaaagt cgcgtcaaag cccacaaggc ggccgccctc cgccagttgg
    13861 gcgatcaatt gctcgggcca aacttcaaaa gcgccgttca ccacaatcgc gtcgaacggc
    13921 gctttggcgg ggagcccggc cggcaactcg ccggcgacga catcgacgcc cggccgtccg
    13981 gcgagcagaa gatttgcgcc ctgcccggat gcggcggtct ccagagccgt cacccgcgcg
    14041 cccagacgcg agagaatggc ggcgccgtaa cccgagccgc cggcgacatc gaggattcgc
    14101 tcgccgggct gcggcttggc cgcctggagg agccggccaa gcgaggccgg ggcgagcagg
    14161 agccgtccgg ctccctgcaa agccggaacg tcgcggtcaa gataagccag cgaagcgaaa
    14221 gccggctcga cgaacgcctc gcgcggcgtt tcggcgaagg cggcgagcag gggcaagtcg
    14281 ttgacgccgg aagggcgcaa ctgattctcg atcatccgcc agcggcgatc gccatagatt
    14341 tccggagagg tcggcctgtt catcggatcg cgattcgtcc cgcccggctt ttccgcccca
    14401 attcgaaaag caaatggagg cctcgcccgg aatcgaaccg gggtgcaagg atttgcagtc
    14461 ctctgcgtag ccactccgcc acgaggccgc gccggccaaa ccgcgccgcg acgcgtggcg
    14521 ctataccaag atttctccgc cagcggcaag ccgattgcag cggcttcggc cgaacgagct
    14581 ctcgctcgac ggcccggacc ccgccaaaac acgatgcgca aaaaatgcgc attgcgcgga
    14641 agattggact gagccgagac gaatggcctg atccttgcct gcaaaacgcg gccattggcc
    14701 taagctcaag ccaaaagcgg catccgcctc gcgcccgccg cccctactct tccgcccaga
    14761 aggagtgccg ctggagcggc aaggcgatga aataaacagc cgcggcggcg agcccggttt
    14821 tgacccaaaa gccgggcgcc aggtccttcc ataccgcgat gacggcgcag acgccccaga
    14881 gcagcgtcgc cgccaacgtc gccgcgcgca tcttctcgac ccgcatcggg tggacgaaga
    14941 cgaccggcgc gaacatcaag ccggccgcgg cgaccaggat cgccgcattg accaggctcg
    15001 gcagcctgaa gacgaaaagg taaaaggcga gcacgttcca cagagccgga aagccgcgga
    15061 accaattgtc cgcggtcttc atccttgtgt cggcgaaata gagcgccgag gcgatgatca
    15121 cggcgagccc gatccagaag gcgatcggcg tcggcatcag gtcggagcgc caaatcgcaa
    15181 cgaccggcac cagaacgtaa gagagaaaat cgacgacgag atcgagaacc acgccgttaa
    15241 tggtcgcggc gacctcggcg acatgggcgg cgcgggcgag cgttccgtcg atcccatcga
    15301 tgaagatcgc caggcccagc caggcgaaga cggcggtgaa atcgcgctcg atcgccgcat
    15361 agagggcgag gactgcgacc gcgccgccgg acgcggtcag cacatgaacc gaaaaggcgg
    15421 cggctcgccg aacgggaacg cggtttttat ccctgaccac gctcatcccg ctcaagccgc
    15481 ccctcccgcc gcgatcttag gcggcgtccg cctggacgga agaaagcacg catgcgaaaa
    15541 atcggcaatg ctcgacagcc agcgtcaacc agcgatcacg gcggagattc tggtcgcggg
    15601 cgccggcctt gcgggattgg cggcggcgat cgccttcgcc aaagcaggct tcgagacggt
    15661 cctttgcggt tcgccggacc gcgccgcgcc cggccgcacc gtcgccttgc tcgacggctc
    15721 gatgcgcttt tttgagcgac tggggctgcg cgccgagatc gagcggctgg gcgcaccgct
    15781 caaggccttg cgcctcatcg acgatacgga ttcgctgtgg cgcggcccgg tcgtcgagtt
    15841 tcatgccgag gagatcgggc tcgaggcttt cggttggaat atcgaaaatt cggctctgac
    15901 cgacgcgctc tgggcggcgg cgggccgcct tccgggcctg acggcgatcg ccagccgaat
    15961 cgcccgctat gaatggggcg cgacgcgcgc gctgacgcaa tgccaggacg gccggctcgt
    16021 cgccgcgcgc ctcgtcgcgg cggccgacgg gcgggattcg ccggcgcggc tttccgccga
    16081 tattccgatc cgcgcccgcc ccctggccca gaccgcgctc actgtctttc tcggccatac
    16141 gcggccgcac cgcgatttct cgaccgaatt ccatacgcgg caggggccgt tcactttggt
    16201 gcccttgccg ccggccggcg agagcgcgca tcgctcgagc cttgtctggg tcatgacccg
    16261 ccagcaggcg gcccgccgca tggctctcgc cgacgacgcg ctggccgccg agatcgaggc
    16321 gcaggcctcg tccatccacg gcgcgatgcg gatcg
//
LOCUS       DATGJW010000346         2047 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1514218, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000346 DATGJW010000000
VERSION     DATGJW010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2047)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2047)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2047
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1514218"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..415)
                     /locus_tag="VKU03_10565"
     CDS             complement(<1..415)
                     /locus_tag="VKU03_10565"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71751.1"
                     /translation="MMKNETIALAAMMAGKAAAGDDVAELIFADNEQAAHFADAAVKY
                     LAAAPSARPADVQRFGAAKGLHLIPAPTPNEALSEEIVTFFSAFTGAAKGMLAANAPK
                     PSAATVQPAAAPARFDNALTERVGDDEDGEGRSESL"
     gene            complement(412..1488)
                     /locus_tag="VKU03_10570"
     CDS             complement(412..1488)
                     /locus_tag="VKU03_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024755.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toprim domain-containing protein"
                     /protein_id="HLJ71752.1"
                     /translation="MTPARLSPSDLKAMLADRIDELACCAAPDGKRKGRYWLAHSPLR
                     IDKEPSFAIWLTGAGAGAFKDFADPEIRGDAIDLIGACVLKRGDFKSREARTQALAWA
                     RDFLGLSGQAPEAARDLAKQARERKRERDLEAARDEARRRRRALEIWGEGQAMSGTAG
                     EAYLLSRGIDARAIANLEHFLRFHPRLSHPHEPHVGPAMLAGFKDPAGGFAAVHCTYL
                     REDGRGKAEVPKAKIIRGGYAGCAIHVSRGPSNLRAIEAASAGVKGMLAITEGIEDGL
                     TIAAERPEWRVWAAGSLGNIGELTPPECADALMVCADADVKQQAIAALNLAVKRLEAF
                     GLPLGVAYATGGAKDFNDLLRGKK"
     gene            complement(1485..1871)
                     /locus_tag="VKU03_10575"
     CDS             complement(1485..1871)
                     /locus_tag="VKU03_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71753.1"
                     /translation="MTRKITLRQQIEEIAALIGEPIRERASVAELRLKRREAIKATLE
                     WLSANEAAVRAAARAKVSWRGAASAQPDIVWNGEQPRRPKGLSRALAASGGARSAPLT
                     TLSAGAFGPAFQTMAGCALALSGSGA"
     gene            complement(1868..>2047)
                     /locus_tag="VKU03_10580"
     CDS             complement(1868..>2047)
                     /locus_tag="VKU03_10580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71754.1"
                     /translation="EIIDMLYGDRADGGPENTEKSIDVVLYRARIIAAALSIAIWSEY
                     RGWRAAPVGWREAAE"
BASE COUNT          279 a          766 c          665 g          337 t
ORIGIN      
        1 ccaagctctc gctccgcccc tcgccgtcct cgtcgtcgcc gacacgctcg gtcagcgcat
       61 tgtcgaaccg cgctggcgcg gccgcaggct gcaccgtcgc cgcggagggc ttcggcgcgt
      121 tcgccgcgag catgcccttc gccgcgccgg taaaggcgga aaagaacgtc acgatttctt
      181 cgctgagcgc ttcgttcggc gtcggcgcag ggatcagatg cagccccttc gccgcgccga
      241 accgttgcac gtccgcgggg cgggccgaag gcgcggccgc cagatacttc accgccgcgt
      301 cggcgaaatg cgccgcctgc tcgttatccg cgaagatcag ctcggcgacg tcgtcgcccg
      361 ccgccgcctt gccggccatc atcgccgcca gcgcgatcgt ctcatttttc atcatttttt
      421 gccccttaag agatcgttga agtccttcgc cccgccggtc gcgtaggcga cgccgagcgg
      481 caggccgaac gcctcgagcc gcttcaccgc cagattgagc gccgcgatcg cctgttgctt
      541 gacgtctgcg tcggcgcaga ccatcagcgc gtcggcgcat tccggcggcg tcagctcgcc
      601 gatattgccg agcgagccgg ccgcccagac gcgccattcc ggccgctcgg cggcgatcgt
      661 cagcccgtcc tcgatcccct cggttatcgc cagcatgccc ttgacgccgg ccgaagccgc
      721 ctcgatcgcc cgcaggttcg acggcccgcg cgagacatgg atcgcgcagc cggcatagcc
      781 gccgcggatg atcttcgctt tcggaacttc ggccttgccg cgcccgtcct cgcggagata
      841 cgtgcaatgg accgcggcga agccgccggc gggatccttg aagccggcca gcatcgccgg
      901 cccgacatgc ggctcgtgcg gatggctgag ccgcggatga aagcgcagga aatgctcgag
      961 attggcgatc gcgcgcgcgt cgatcccgcg cgacaggaga taggcttcgc ccgccgtccc
     1021 gctcatcgcc tggccttcgc cccagatttc gagcgcccgc cggcggcggc gcgcttcgtc
     1081 ccgcgccgcc tcgaggtcgc gctcgcgctt gcgctcgcgc gcttgtttgg cgagatcgcg
     1141 cgccgcctcc ggcgcctggc cggaaaggcc gaggaagtcg cgcgcccagg ccagcgcttg
     1201 cgtccgcgcc tcgcgcgact tgaagtcgcc gcgcttcaaa acgcaggcgc cgatcaggtc
     1261 gatcgcgtcg cccctgatct ccggatcggc gaaatccttg aacgcgcccg cgcccgcgcc
     1321 ggtcagccag atcgcgaagg acggctcctt gtcgatcctc agcggcgaat gcgccagcca
     1381 atagcgcccc ttgcgcttgc cgtccggcgc cgcgcagcaa gcgagctcgt cgatccggtc
     1441 ggcgagcatc gccttgaggt cggacgggct gaggcgggcg ggggtcatgc gccgctcccc
     1501 gaaagagcaa gcgcgcaacc cgccattgtt tggaacgctg gcccgaatgc gccggccgaa
     1561 agggttgtca agggcgcgct ccgcgcgccg ccggaggcgg ctaaagccct tgacaaccct
     1621 ttcggccggc gcggctgctc gccgttccaa acaatgtcgg gttgcgcgct tgccgcgccg
     1681 cgccagctta ccttcgccct cgccgccgcg cgcaccgccg cctcgttcgc gctgagccat
     1741 tccagcgtcg ccttgatcgc ctcgcgccgt ttcagccgca gctcggcgac gctcgcacgc
     1801 tcgcggatcg gctcgccgat cagggccgcg atctcctcga tctgctggcg gagcgtgatc
     1861 ttgcgggtca ttccgccgcc tcgcgccagc cgacgggcgc ggcgcgccag ccgcgatatt
     1921 ccgaccagat cgcgatcgac aaagccgccg cgatgatgcg ggcgcgatag agcacgacgt
     1981 cgatcgattt ttcggtgttt tccgggccgc cgtcggcgcg gtcgccatac agcatgtcga
     2041 ttatttc
//
LOCUS       DATGJW010000347         3641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_583767, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000347 DATGJW010000000
VERSION     DATGJW010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3641
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_583767"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(66..431)
                     /locus_tag="VKU03_10585"
     CDS             complement(66..431)
                     /locus_tag="VKU03_10585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007771138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ71755.1"
                     /translation="MKAPDFLLLYVDNPPASAAFYEKLLARAPVENSVNFSMFVLDSG
                     LKLGFWRTDAVEPKPTARPGGAEIVFALDGAEAVDALCADWRKKGLPIAQEPTAMDFG
                     YTFVALDPDGHRLRACALA"
     gene            complement(501..1196)
                     /locus_tag="VKU03_10590"
     CDS             complement(501..1196)
                     /locus_tag="VKU03_10590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HLJ71756.1"
                     /translation="MSRAQRLLDLLQVLRRHRRPVTGAALAGEVGVSLRTLYRDIAAL
                     QAQGADIRGEAGLGYVLKPGFTLPPLMFTSEEIEALALGSQWVAARADRRLAGAARNA
                     LAKIAAVTPPELADVLDSSTLIVASRGDAAAEAHLPILRDAIRQERKVSIAYVDAVGG
                     HSERVIWPFALAFFDQARVVVAWCEKRVAFRHFRTDRIAALTTLDGRYPRRRAALLKE
                     WRAAERISPPDGN"
     gene            complement(1354..1953)
                     /gene="rsmD"
                     /locus_tag="VKU03_10595"
     CDS             complement(1354..1953)
                     /gene="rsmD"
                     /locus_tag="VKU03_10595"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HLJ71757.1"
                     /translation="MRIVGGRLRGRVLKAPASRDIRPTSERLRESIFDILVHAYGDPV
                     AGARVIDLFAGSGALGLEALSRGAGFALFVDNGAEARALLRANIETLGLGGATRVWRA
                     DAMRLGLAPAAYSLAFLDPPYGKKLAAPALATLVQGGWLAPGAIVVVEEAARVAIAAP
                     EALRLAEERVYGDTKIAILIMEGTSDAVLPREAGEGDRA"
     gene            complement(1957..3213)
                     /locus_tag="VKU03_10600"
     CDS             complement(1957..3213)
                     /locus_tag="VKU03_10600"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HLJ71758.1"
                     /translation="MSDQPPKSASARRGQKPPPAKRHPGERIAKAMARSGLCSRRDAE
                     AWIAAGRVSVNGEALTSPAFNVTERDDIRVDGAPLARRERTRLFLFHKPRGLVTTARD
                     PEGRPTIFDALPENAPRLVAVGRLDINTEGLLLLTNDGGLARILELPATGWLRRYRVR
                     AHGAVEQAALDRLAEGVTIDGVAYAGIEAKLDRAQGANVWLTMGLREGKNREIKRVLE
                     HLGLSVNRLIRVSFGPFQLGELEEGALAELPTRIVREQIGPRLAREAGADFDAPLREK
                     PTKTRGAPAHSRPARERPGARRKDHASAVAGVGRREAAPPREERKPERKRRYFAALRA
                     ERDEAERGPRKRLERGAISDRKGREIAVERFQQAERKEMRGMAPRGPQKRRFEPQPPQ
                     ARPRPDGPRGSKPNRRGGGRRGPKRG"
     gene            complement(3323..>3641)
                     /locus_tag="VKU03_10605"
     CDS             complement(3323..>3641)
                     /locus_tag="VKU03_10605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2244 domain-containing protein"
                     /protein_id="HLJ71759.1"
                     /translation="VLLLYLAFRANFRAARAYELLRLTSLELLFARVSSRGERKEWRF
                     NPVWVRFERVEHEEFGTQRLALVSQGKSVEVGSFLGPDQKAALARSFSVALAKARRGP
                     RFS"
BASE COUNT          571 a         1265 c         1290 g          515 t
ORIGIN      
        1 gcgcagcccc tcatccggcc gagcttcgct cggccacctt ctccccgcct tgcggggaga
       61 aggaattaag ccagcgcgca ggcccgcagg cggtgaccgt cggggtcgag cgcgacgaaa
      121 gtgtagccga aatccatcgc ggtcggctct tgcgcgatcg gcaggccttt cttgcgccaa
      181 tcggcgcaga gcgcgtcgac ggcttcggcg ccgtcaagcg cgaagacgat ctcggccccg
      241 cccggccggg ctgtcggctt tggctcgacc gcgtccgtcc gccagaagcc gagcttgagg
      301 ccgctgtcga gaacgaacat cgagaaattg acggagttct cgaccggcgc ccgcgccagc
      361 agcttctcgt aaaaggcggc gctggcgggc ggattgtcga catagagcag gagaaaatca
      421 ggggctttca tggcgaacct ctcaaaggtc aagcggcggg cgaagcacgc cgtcggcccg
      481 cctcttagga ggccggcgcg tcagtttccg tcaggaggcg aaatcctctc ggccgcccgc
      541 cattccttga gcaatgcggc gcggcggcgc ggatagcggc cgtcgagcgt cgtcagcgcg
      601 gcgatgcggt cggtgcggaa atggcggaag gcgacgcgct tttcgcacca ggcgacgacc
      661 acccgggcct ggtcgaaaaa agcaagcgcg aaaggccaga tgacccgctc gctatggccg
      721 ccgacggcgt cgacataggc gatcgacact ttgcgctcct ggcggatcgc gtcgcggaga
      781 atcggcaaat gcgcctccgc cgccgcgtcg ccgcgcgacg ccacgatcaa agtcgaggaa
      841 tcgagcacgt cggccagttc gggcggcgtc accgcggcga tcttggcgag ggcgttgcgc
      901 gccgcgccgg cgagcctgcg gtcggcccgc gccgccaccc attgcgagcc gagcgccagg
      961 gcttcgatct cttccgacgt gaacatcagg ggcggcaggg tgaagcccgg cttcagcaca
     1021 tagccgaggc cggcttcgcc gcgaatatcc gcgccttgcg cctgcagggc ggcgatgtcg
     1081 cgataaagcg tgcgcaggct gacgccgacc tccccggcca aagccgcccc ggtcaccggc
     1141 cggcgatggc ggcgcaggac ttgaaggaga tcgagcaggc gttgcgcgcg agacatggcg
     1201 gcggaaattc gcatgggcgg aagaacgcgc caagcgccct ttcctccccc gtgaagcgaa
     1261 gcgaacgggg gagggggacc ggccaaaggc cggtggaggg gggccgccgg cggttgagga
     1321 tgatcgtctg gcgcagctgc cccctccacc gcgctacgcg cggtccccct cccccgcttc
     1381 gcgggggagg acggcgtcgc tcgtaccttc cataatcaag atcgcgattt tcgtatcgcc
     1441 atagacccgc tcttcagcga gccgcagcgc ttccggcgca gcgattgcga ctcgcgccgc
     1501 ttcctcgacc acgacgatgg cgcccggcgc cagccagccg ccttggacaa gcgtcgcgag
     1561 cgccggcgcg gcgagcttct tcccgtaggg cggatcgaga aaggcgagcg aataggccgc
     1621 cggcgccaag ccgaggcgca tcgcgtcggc gcgccagacg cgcgtcgcgc cgccaaggcc
     1681 gagcgtctcg atgttggccc gcagcaaagc gcgcgcttcg gcgccgttat cgacgaatag
     1741 ggcgaagccg gcgccgcgcg acagagcttc gaggccgagc gcgcctgacc cggcgaaaag
     1801 atcgatgact cgggccccgg cgaccgggtc gccataggca tggacgagaa tgtcgaaaat
     1861 cgattcgcgc agccgctccg aagtcggccg gatgtcgcgc gacgccggcg ccttgagaac
     1921 gcggccgcgg agccgcccgc cgacaatgcg caacgctcag ccgcgcttcg ggccgcgccg
     1981 gccgccgccg cgcctattcg gcttcgagcc gcgcggaccg tcggggcgag ggcgcgcttg
     2041 cggcggttga ggctcgaacc gccgcttctg cgggccgcgc ggcgccatgc cgcgcatctc
     2101 ctttcgctcg gcttgctgga aacgctcgac ggcgatctcg cgtcccttac ggtcgctgat
     2161 cgcgccgcgc tccagccgct tccgggggcc gcgctcggct tcgtcccgct cggcgcgaag
     2221 cgcggcgaaa taccgccgct tgcgctccgg cttccgctcc tcgcgcggcg gcgcggcctc
     2281 gcgacggccg acgcccgcaa cggccgacgc atgatccttt cgccgcgcgc ctggacgctc
     2341 gcgcgccggg cgcgaatgcg ccggggcgcc gcgcgtcttc gtcggctttt cccgcaacgg
     2401 cgcgtcgaaa tcggcgccgg cctcgcgcgc cagcctgggc ccgatctgct cgcgcacgat
     2461 gcgcgtcggc aattccgcca aggcgccttc ttcgagctcg ccaagctgga aggggccgaa
     2521 cgagacccgg atcaggcgat tgaccgagag gccgagatgt tcgagcacgc gcttgatctc
     2581 gcgattcttg ccctcacgca ggcccatcgt cagccagaca ttcgcgccct gcgcgcggtc
     2641 gagcttggct tcgatcccgg cataggcgac gccgtcgatc gtgacgccct cggcaagccg
     2701 gtcgagcgcc gcctgctcga cggcgccatg ggcgcgcacg cgatagcggc gcagccagcc
     2761 ggtggcgggc aattccagga tgcgggcgag gccgccgtca ttggtcagga gcagcagccc
     2821 ctcggtgttg atgtcgagcc gcccgaccgc gacgaggcgc ggcgcattct cgggcagcgc
     2881 gtcgaaaatc gtcggccggc cttccggatc gcgcgcggtc gtcaccaagc cgcgcggctt
     2941 gtggaacagg aaaagccgcg tgcgctcgcg ccgggcgagc ggcgcgccgt cgacgcgaat
     3001 gtcgtcgcgc tcggtgacgt tgaaagccgg actggtcagc gcctcgccgt tgaccgagac
     3061 gcggcccgcc gcgatccagg cctcggcgtc gcggcgcgag cagaggcctg accgcgccat
     3121 cgccttggcg atgcgctcgc cgggatggcg cttggcgggc ggcggctttt gcccgcgccg
     3181 cgcgctggcg cttttgggcg gctgatcgct catcccgagc gttttacggc aaatcggcgg
     3241 gagggcaatg gcggcgcgac ccgttcattg aaaaggagcg cgcgctgttg ttctcgggtg
     3301 gaaatgttaa ggcggatgaa cgctacgaaa acctcggccc gcggcgcgcc tttgcgagcg
     3361 cgaccgagaa agatcgcgcc agcgccgcct tctgatcggg gccgaggaag ctgccgacct
     3421 cgacgctctt gccttgcgag accagcgcca ggcgctgcgt gccgaattcc tcgtgctcga
     3481 cgcgctcgaa gcggacccag accggattga agcgccattc cttgcgctcg ccgcgcgacg
     3541 agacgcgcgc gaaaagcagt tcgagcgagg tcaagcgcag caattcatag gcgcgcgcgg
     3601 cgcggaaatt ggcgcgaaac gccaggtaga ggaggagcac g
//
LOCUS       DATGJW010000348         5938 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1104517, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000348 DATGJW010000000
VERSION     DATGJW010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5938)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5938)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5938
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1104517"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(129..1064)
                     /locus_tag="VKU03_10610"
     CDS             complement(129..1064)
                     /locus_tag="VKU03_10610"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase"
                     /protein_id="HLJ71760.1"
                     /translation="MILISGEALVDLIPDRVREGAYDVVLGGSPYNVAIGLGRLGAPT
                     AFVSRLSKDANGEAFAATLAKTGIDLSFIERVAEPTTLAFVMRGTAETGARYSFYLDA
                     TSFDGPWPFPAQWPAGARHLHVGSISALDARHGERVVAALSEARKTATTSFDPNIRPL
                     VTPDRAKVIALVERQLALASFVKASDEDLLWLYPDRDPETSIADWARRGPFFAVITRG
                     AGGAAAYLGASRIEARAPKIAVVDTVGAGDSFMSALIAAMDADGALGAGAARPSETSL
                     SRWLAFAAAAAAITCTRKGSDPPTRAEVEARLARG"
     gene            complement(1241..2680)
                     /locus_tag="VKU03_10615"
     CDS             complement(1241..2680)
                     /locus_tag="VKU03_10615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HLJ71761.1"
                     /translation="MNDVNLNFIAGEWVAGDGASRDINPSNTNDVIGEFTRASAAQVE
                     TAIAAAKAAFPAWSHSTPQERHDILLRASTEILARREELGRLLSREEGKTLAEGIGEA
                     TRAGQIFDFFAGEALRIPGEKLASVRPNVDVELTREPVGVIGIISPWNFPIAIPAWKI
                     APALAYGNCVVFKPADLVPGTAHALSEIVIRAGAPKGVFNLAMGRGSVVGEAILQHKH
                     IDAITFTGSVQTGRRVAAVCAEHMRKCQLEMGGKNPLVVLDDADLKVAVECAVNGAFF
                     STGQRCTASSRLIVTKGIYAKFAEAVTERMKSLVVDDALKSGTHIGPVVDQGQLDQDL
                     SYLKIGVGEGAKLVAGGERVERGAPGFYLTPALFTDVDNKMRIAREEIFGPVAAMIPA
                     KDYDEALALANDTPFGLSSGICTTSLKHATHFKRNAEAGMVMVNLPTAGVDYHVPFGG
                     RKGSSLGSREQGRYAAEFYTTVKTAYTLA"
     gene            complement(2921..4099)
                     /locus_tag="VKU03_10620"
     CDS             complement(2921..4099)
                     /locus_tag="VKU03_10620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLJ71762.1"
                     /translation="MAQLQLSVAETAPTDLYRGALAARVWRPDLDGPSVAAIRAEGVV
                     DISRRFPTMSALCEAEDPAGALRAAAGEPIASFEAILANTPAESRDPNKPWLLAPVDL
                     QALKAAGVTFATSMLERVIEERARGDLNAAASLRAELTGLIGEDLSKLKPGSPAAMRV
                     KEALIARGAWSQYLEVGIGPDAEIFTKSQPMSAVGHGADAGFHHASRWNNPEPEVVVV
                     VNSRGRSVGASLGNDVNLRDFEGRSALLLGKAKDNNASCAIGPFLRFFDETFSMDDIR
                     RMEVRLTVEGPEGFRLDGGSSIAKISRDPEDLVSQMIGPSHQYPDGAALFLGTMFAPI
                     ADRDAPGKGFTHKEGDIVAVAADKLGKLANRMRHSRDCPPWTFGAGALMKNLAKRGLL
                     "
     gene            4303..4980
                     /locus_tag="VKU03_10625"
     CDS             4303..4980
                     /locus_tag="VKU03_10625"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ71763.1"
                     /translation="MSEAEARRGSKADQAYHDLKEAILSGALEPGEAIDKLALCERLG
                     VSRFPVSSAINRLAYEKLVAIEPQHGSFVAKISVEDVRELMMVRRALESEIAAQAALS
                     LPREGVEELERNLRYQLAAVEARDLAGFYALDVDFHRLITTQLGLSRTGEILDGLRSH
                     LERVRRLLMTPPGRLPRTYAEHNAIAKAIARGDAEGARAAMRDHLVETTELFESFAKA
                     RPNLFSS"
     gene            5010..>5938
                     /locus_tag="VKU03_10630"
     CDS             5010..>5938
                     /locus_tag="VKU03_10630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UxaA family hydrolase"
                     /protein_id="HLJ71764.1"
                     /translation="MSSPRALRLNAEDNVVIAVDEIRAGDQPRNAPAALARVPKGHKM
                     ASAPIAAGAPILKFGQIIGFASKPIAPGEWVHEHNVEMHDFARDYRFCEAAKPEALLP
                     DERRATFQGFRRANGKAGTRNYVAILTSVNCSATVARLIAREAERSGMLDDYPNVDGV
                     IALVHGTGCGMAAKGEGFEILKRTQWGYAANPNVGGVLLVGLGCEVFQIGRMKEEYGI
                     VEGELFRTMTIQATGGTKKTIEAGLGYIGEMLPVVNRAKREPIPASELMLALQCGGSD
                     GYSGITANPALGVAADILVRHGGTAILSETPEI"
BASE COUNT          989 a         1976 c         2004 g          969 t
ORIGIN      
        1 gaaatctcga aatcgcgtta tttcacgaga cttcgtcgac gacgatgcga ctcgcctctt
       61 actttccccg gacagccctg cgcagcgggg agaagggatc cccggcccgc ccggcgggcg
      121 gtacctcgct accccctcgc caggcgcgcc tcgacctcgg cccgggtcgg cggatcggag
      181 cctttgcgcg tgcaggtgat cgccgcggcg gccgccgcga aggcgagcca gcgggaaagg
      241 ctcgtctcgc tgggccgggc ggcgcctgcg ccaagcgcgc cgtccgcgtc catggcggcg
      301 atcaaagccg acatgaagct gtcgccggcg cccaccgtgt cgaccaccgc gatcttcggc
      361 gcgcgcgcct cgatgcggct cgcgccgaga taggcggccg cgccgccggc gccgcgcgtg
      421 atgacggcga aaaacggtcc gcgccgcgcc cagtcggcga tcgaggtttc cggatcgcga
      481 tcgggataga gccaaagcaa atcttcgtcg ctcgccttga cgaagctcgc cagcgcgagt
      541 tggcgctcga ccaaagcgat cactttcgcg cgatccggcg tcaccagcgg ccggatgttc
      601 ggatcgaagc tggtcgtggc ggtcttgcgc gcctcgctca gcgcggcgac gactcgctcg
      661 ccgtggcgcg cgtccaaagc cgagatcgag ccgacatgca gatggcgggc gccggccggc
      721 cattgcgctg gaaagggcca gggcccgtcg aacgacgtcg catcgagata gaaggaatag
      781 cgcgcgccgg tctcggcggt gccgcgcatg acgaaagcga gcgtcgtcgg ctcggcaacg
      841 cgctcgatga aggagaggtc gattccggtc ttggcgagcg tggcggcgaa agcctcgcca
      901 ttggcgtctt tggacaagcg cgagacgaaa gcggtcggcg cgccgaggcg gccgaggccg
      961 atcgcgacat tgtacggcga gccgccgagc accacgtcat aggcgccttc ccggacgcgg
     1021 tcgggaatga gatcgaccag cgcctcgccg gaaatgagta tcatgccccc tgcccctccc
     1081 ggttatttcc tcccccgcga accgggggag ggggaccatg cgaagcatgg tggagggggc
     1141 cacggcctgt caggataatc ctcaaccgcc gcggccgccg cggcccccgc caccgcgccg
     1201 acgcgcggtc cccctccccc gtctgcgacg ggggaggata ttacgccagc gtgtacgcgg
     1261 tcttcaccgt cgtatagaac tccgccgcat agcggccctg ctcgcgcgag ccgaggctcg
     1321 agcctttgcg gccgccgaac ggcacgtggt agtcgacgcc cgcggtcggc aggttgacca
     1381 tgaccatgcc ggcctcggcg ttgcgcttga aatgcgtcgc gtgtttcagg ctggtcgtgc
     1441 aaatgcccga gctcaagccg aacggcgtat cgttggcgag ggcgagggcc tcgtcatagt
     1501 ccttggccgg gatcatcgcc gcgaccgggc cgaaaatctc ctcgcgcgcg atgcgcatct
     1561 tgttgtcgac atcggtgaac aaagccggcg tcaaatagaa gccaggcgcg ccgcgctcga
     1621 cgcgctcgcc gccggcgacc agcttggcgc cttcgccgac gccgatcttg aggtaagaga
     1681 gatcctggtc gagctggccc tgatcgacga ccggtccgat atgggttccg ctcttcaggg
     1741 cgtcgtcgac gacgaggctc ttcatccgct cggtcaccgc ctcggcgaat ttcgcgtaga
     1801 ttccctttgt gacgatcagc cgcgaggagg ctgtgcagcg ctggccggtc gagaagaagg
     1861 cgccgttgac ggcgcattcg accgcgacct tgaggtcggc gtcgtcgagc acgacgagcg
     1921 gattcttgcc gcccatttcg agctggcatt tgcgcatatg ctcggcgcag acggcggcca
     1981 ccctgcgccc ggtctgcacc gagccggtga aggtgatcgc gtcgatatgc ttatgttgca
     2041 agatcgcttc gccgacgacc gagccgcgcc ccatcgccag gttgaacacg cctttggggg
     2101 cgccggcgcg gatcacgatt tcggagaggg catgcgcggt gccggggacg agatcggccg
     2161 gcttgaacac gacgcaattg ccataggcga gcgccggcgc gattttccag gccggaatgg
     2221 cgatcgggaa gttccagggc gaaatgatcc cgatcacgcc gaccggctcg cgcgtcagct
     2281 cgacatcgac attgggcctg acgctggcga gcttctcgcc gggaatgcgc agcgcctcgc
     2341 cggcgaagaa atcgaaaatc tgcccggctc gcgtcgcttc gccgattccc tcggcgagcg
     2401 tcttgccttc ctcgcgcgac agcagccggc cgagctcctc tctgcgggcg aggatttcgg
     2461 ttgaggcgcg caacaggatg tcgtgccgct cctgcggcgt ggaatgcgac caggccggga
     2521 aggccgcctt ggccgccgcg atcgccgtct cgacttgcgc cgcgctggcg cgggtgaatt
     2581 cgccgatcac gtcgttcgtg ttggagggat tgatgtcgcg cgaggcgccg tcgcccgcca
     2641 cccactcgcc ggcgatgaag ttcagattga cgtcgttcat gctgcgctcc taacaagctc
     2701 taagatagct cagagaccgc ggctcaccct caccccaacc ctctcccgca agcgggagag
     2761 ggggcgctcc gcagccttca tcagccgccg tgctgtagcc gcgccgacgc ggctacaaat
     2821 cccttctccc gcttgcggga gaaggtggcg cgaagcgccg gatgagggtg agctgcggtc
     2881 ttgatcatta cgcttcagcc agatgaaccc cgctcaagcg ctaaagcaag ccccgcttgg
     2941 cgaggttctt catcagcgcg ccggcgccga acgtccaggg cgggcaatcg cgcgaatggc
     3001 gcatgcggtt ggcgagcttg ccgagcttgt cggcggcgac ggcgacaata tcgccctctt
     3061 tgtgcgtgaa gcccttgccc ggcgcgtcgc gatcggcgat cggcgcgaac atcgtgccga
     3121 ggaacagcgc cgccccgtcg ggatattgat ggctcggccc gatcatctgg ctgacaaggt
     3181 cttccgggtc ccggctgatc ttggcgatcg acgagccgcc gtcgagccgg aagccttccg
     3241 gcccttcgac ggtgagacgg acctccatcc ggcgaatgtc gtccatcgag aacgtctcgt
     3301 cgaaaaagcg caggaacggg ccgatcgcgc agctcgcatt gttgtccttg gccttgccga
     3361 ggagcaaggc ggagcggccc tcgaaatcgc gcaaattgac gtcgttgccg agactcgcgc
     3421 cgaccgaccg cccgcgcgaa ttgacgacga cgaccacttc cggctccggg ttgttccagc
     3481 gcgaagcgtg gtgaaagccg gcgtcggcgc catggccgac cgccgacatc ggctggctct
     3541 tggtgaaaat ctcggcgtcc ggcccgatgc cgacttcgag atattgcgac caggcgcccc
     3601 tggcgatcag cgcctctttc acgcgcatcg cggcgggcga gccgggcttg agcttggaga
     3661 ggtcttcgcc gatcaggccg gtcagttcgg cgcggagcga ggcggcggcg ttgaggtcgc
     3721 cgcgcgcccg ctcttcgatg acgcgctcca gcatcgaggt ggcgaaagtg acgcccgcgg
     3781 ccttgagcgc ctgaagatcg accggggcaa gcaaccatgg cttgttcgga tcgcggcttt
     3841 cggccggcgt gttggcgaga atggcctcga acgaagcgat cggctcgccg gcggcggcgc
     3901 gtagcgcccc cgccgggtct tccgcctcgc agagggcgct catcgtcgga aagcggcggg
     3961 aaatgtcgac gacgccctcg gcccggattg cggcgacgga cggcccgtcc agatcgggcc
     4021 gccagacccg cgcggcgagc gcgccgcggt aaagatcggt cggcgccgtc tcggcgacgc
     4081 tcaactgaag ctgggccatc tttcctcctg ttcgtcgtgg ccgcggtcgg cgatcgcggc
     4141 taccgcccgt cgcccttggg cagtgcgccc gcgacatttt acgccggcgc cgcttctctt
     4201 tacgcacggc gacattgacg caggcgcttc ctctggttag cgtctaacat gctagcttcg
     4261 aaagaaaaga cttttgtggg cggcgacgca agaggcggac ggatgagcga agcggaggcg
     4321 cggcgcggct ccaaggccga ccaagcctat cacgatctga aagaggcgat tctgtccggc
     4381 gccttggagc cgggcgaggc gatcgacaag ctcgcgctgt gcgagcggct cggcgtgtcg
     4441 cgatttcccg tctcctccgc gatcaatcgg ctggcctatg agaagctggt ggcgatcgag
     4501 ccgcagcacg gctccttcgt cgccaagatt tcggtcgagg acgtgcgcga attgatgatg
     4561 gtgcgccgcg ccttggagtc ggagatcgcc gcacaggcgg ctttgtcgct gccgcgcgag
     4621 ggggtcgagg aattggagcg taacctgcgc taccagctcg ccgccgtcga ggcgcgcgat
     4681 ctcgccggct tctacgccct ggatgtggac ttccatcgcc tgatcacgac gcagcttggc
     4741 ttgagccgca ccggcgaaat tctcgacggc ctgcgctcac atctggaaag ggtgcggcgc
     4801 ttgctgatga ccccgccggg gcgcctaccg aggacttatg ccgagcacaa tgcgatcgcc
     4861 aaggcgatcg cgcgcggcga cgccgagggc gctcgcgccg caatgcgcga ccacctcgtc
     4921 gaaacgaccg aattgttcga gagtttcgcc aaagcccgcc cgaacctgtt ctcctcttaa
     4981 agaagcgacc caccgccgga acgaagaaaa tgagcagtcc acgcgccctg cgcctcaacg
     5041 ccgaagacaa tgtcgtgatc gcggtcgacg agatccgcgc cggcgaccag ccgcgcaacg
     5101 ccccggccgc gctcgcccgc gtgcccaagg gccataaaat ggcctccgcg ccgatcgcgg
     5161 ccggggcgcc gatcttgaaa ttcggccaga tcatcggctt cgcctccaag ccgatcgcgc
     5221 ccggcgaatg ggtgcacgag cacaatgtcg aaatgcacga tttcgcccgc gactatcgct
     5281 tctgcgaggc ggcgaagccc gaggcgctgc tgcccgacga gcggcgcgcc actttccaag
     5341 gtttccgccg cgccaacggc aaagccggca cgcgcaatta cgtcgcgatc ctgaccagcg
     5401 tgaattgctc ggcgacggtc gcccggctga tcgcccgcga agccgagcgc tccggcatgc
     5461 tcgacgatta ccccaatgtc gacggcgtca ttgcgctcgt ccatggcaca ggctgcggca
     5521 tggcggcgaa gggcgagggt ttcgagattt tgaagcgcac gcaatggggc tatgcggcca
     5581 atcccaatgt cggcggcgtg ctgctggtcg gcctcggctg cgaggtcttc cagatcggcc
     5641 ggatgaagga agagtacggg atcgtcgaag gcgaactgtt ccgcacgatg acgatccagg
     5701 cgaccggcgg caccaagaag acgatcgaag cgggcctcgg ctacatcgga gagatgctgc
     5761 cggtggtcaa tcgcgcaaag cgtgagccga ttccggcctc ggaactgatg ctggcgctgc
     5821 aatgcggcgg ctctgacggc tattcgggca tcaccgccaa cccggcgctc ggcgtcgccg
     5881 cggacattct ggtccgtcat ggcggcaccg cgatcctctc cgagacgccg gaaatcta
//
LOCUS       DATGJW010000349        14843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_896173, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000349 DATGJW010000000
VERSION     DATGJW010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 14843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14843
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_896173"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..845)
                     /locus_tag="VKU03_10635"
     CDS             complement(<1..845)
                     /locus_tag="VKU03_10635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343747.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLJ71765.1"
                     /translation="MARAPDSVLLDLARRLEAGAIDARRLVEQTLKAIEESDPAIFTV
                     VTAERARREAAASDARRAAGRALGPLDGAPIAWKDLFDFAGFVTTAGSRALADAAPAK
                     ADAPVVAALAAAGMAAVGRTNMTEFAYSGIGLNPRFGTPRNPYGSTPRVPGGSSSGSA
                     VAVARGLTPAAIGTDTGGSVRIPAAFTGVVGYKASAGRYPMSGAFPLSKTLDTLGVFA
                     GAVADAILVDAAMRGVTPTLPAPAIVHGLRIVVPTNVVLHDCETEVLANFEGSLATLK
                     AAGVV"
     gene            complement(847..1944)
                     /locus_tag="VKU03_10640"
     CDS             complement(847..1944)
                     /locus_tag="VKU03_10640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71766.1"
                     /translation="MERDLQLVGVGKTYDNGAAAVVDFNLDVAKGEFVAFLGPSGCGK
                     TTTLRMIAGFEIITSGDIFIRGKRINALPAEKRPTAMIFQSYALFPHMTVRGNIAYGL
                     KVKRLDRKTIDERVERIVEKLGLADIIDSRTDRLSGGQRQRVALARSLVIEPDILLLD
                     EPLGALDANLRKTIQGELRLLQRNLGVTFVFVTHAQSEALALSDRIVVMNRGRIEQIS
                     PPHELYRRPQTPFVASFIGRNIIFDGDGVERRGEIALVKTRFGLFSGRAVGEAAGAAK
                     LVVPSEAFAVAPRRDGEAAPDEPGNRIAARIENRERSGNLAALRLALADGTMIRVEGH
                     ADRFAETLTIDQAVWLRWSAEAASVIPAGGG"
     gene            complement(2007..3239)
                     /locus_tag="VKU03_10645"
     CDS             complement(2007..3239)
                     /locus_tag="VKU03_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012649338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ71767.1"
                     /translation="MSETDLSRRSLLKSGAAVAALAIGGGTPLLDSRNAYAAASKLSG
                     ETLRTIGLSVTVQERILADFKAESGVGSTSGTAATFPDAQTKILSGSKDYDCWETIGE
                     RLPAVVQTNNIDAIPASAIKNWSNIRPTFTKPDPKWDRKAQIVGQIWTDDSEKSLWMV
                     PVVYNYDSIGYNPDVVTAEEANTWTAIFDDKWKGKSGLNTDPLIALGQAIMAMNTLGM
                     SQVKNPGNASKAEIAEAMKFLISKKKGGQFRALWGDFGELVNLLASGEMVVCDAWQPA
                     VMAVKAQGKPCKYAVPKEGYRAWAIGPSLIAGSPNKEAVYAYADFWLSGKPGITVSEQ
                     GYYSPSTNIKQAMDPDKYAFWYEGKPWKGKAERGIKEGDLRDGGSLETRAAHVAYWHQ
                     WPDEYDFVIQKWDEFLNA"
     gene            complement(3283..4041)
                     /locus_tag="VKU03_10650"
     CDS             complement(3283..4041)
                     /locus_tag="VKU03_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013169031.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71768.1"
                     /translation="MGQRTILITGGGIGIGRATALAFAAAGDHVVVTDILETEGSSAA
                     QEAEAKGGSAEFIALDVRSTRQADKIVAEIEARRGAIDVVVANAGIAHRTPLADLTDE
                     IWDHTFEIDLKGMLRVIRPALPAMKRKGGGAIGCVSSIMGIAYGWDEHVHYSAAKSGV
                     VGLVRGLAVELARAGIRVNGVAPGYIRTAQLLSKEHSLGPEGAEAAGAFIPMGRIGEP
                     SDVADVIHFLASKAARYMTGQTIVVDGGLLVGRY"
     gene            complement(4052..4801)
                     /locus_tag="VKU03_10655"
     CDS             complement(4052..4801)
                     /locus_tag="VKU03_10655"
                     /EC_number="1.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004112175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose 1-dehydrogenase"
                     /protein_id="HLJ71769.1"
                     /translation="MRRAGQKTIAIVTGGGSGIGRAIARRLAADGAAVTVNDVAKNRA
                     EAVAAEIAAADGRAIAVGGDVASEADVKTIVAAGAAAFGDATLLVNCAGHVHQAPFLD
                     IAPADFDRIFAVHVRGAYLCARAVLPAMLAAKSGVIVNIASQLGQIGGVELAHYSAAK
                     AAIIGLTKSLAREFSPHGVRVNAVAPGPIETPLVMALSEDWRRRKAAELPLGRFGQPE
                     EVAAAVAFLASDEAGLFVGQTLGPNSGDVML"
     gene            complement(4798..5643)
                     /locus_tag="VKU03_10660"
     CDS             complement(4798..5643)
                     /locus_tag="VKU03_10660"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007812632.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71770.1"
                     /translation="MESRLIKWALGGFTALIVVFLYAPFAVLAILSFQTGPEGGPQFP
                     IVAWSVYWYKHLFGLTPPSRIAPLPIESALARSLTLAVMTMIVSTVLGVMAAQAFRTR
                     FKGAGLVFYLIILGMMVPGVLIGLGMALLANTIGLDRHWWSTAFALHVVYTFPFAFLV
                     MLAIFNRFDASVEEASWSLGVSPARTFRKVTFPLIFPGVLSAMLFAFTLSYDEFSRTL
                     FTSGRELTLPLAIYGTFSVEIHPNIFAFGVLTTLFSFALLAIYAILMALSVRRARRLM
                     IQEEA"
     gene            complement(5645..6520)
                     /locus_tag="VKU03_10665"
     CDS             complement(5645..6520)
                     /locus_tag="VKU03_10665"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71771.1"
                     /translation="MESPAAAAQQTRRRVVYWLIAPGVLWMLIFLVLPMAMMIYVSFW
                     TQTTFKIEPILTTRSWVAFFTSETYLDALWTTIRIWLIVLGATLLVGYPTALFIGLFV
                     RNKTLQTALLVVCVIPFWTSFLIRVLAWRPMLGTEGAVNILLMKAGLISQPIEALLYT
                     DLSVIIGMTQIYCVFMVGPIAFMLSRIDPTIIEAARDLGAGFWRIFRTIILPLSMPGV
                     VVGAIFVSVMVLGEFATSAALSGRKVNLLGNIIVTQVGSLRWSFAAVVGVVLTIIMGA
                     AVSILLRVVDLRREL"
     gene            complement(6583..7641)
                     /locus_tag="VKU03_10670"
     CDS             complement(6583..7641)
                     /locus_tag="VKU03_10670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71772.1"
                     /translation="MLQITGLTKRYGGAVALDDVSLDIPRDCYVSLLGPSGSGKTVLL
                     RVIAGFEEPDAGTIEFAGRPIVAAPVYNRGIGFVFQNFALFPHLSVEENVAFGLKNRA
                     GATLGKSEIRDRVRAMIELVGLAGLEARGVHQISGGQRQRVALARTLVTEPPLVLLDE
                     PLGALDANLRTRIRAELRMIRERLGVTFLHVTGSESEALAIGDRVIVLDRGRVVQFDD
                     PDTLFERPASARVARFLNCYNLFGGAVAGASFQSDAGEFPLPRGPCPPRGFYGVRYDR
                     IAVAPRAEKSGADQVSLTAAFVASEFTGPSLLSFFKLASGQIVEVETPLGDRAPPQYE
                     LGEAYALSWRRDGARVFA"
     gene            8070..9092
                     /locus_tag="VKU03_10675"
     CDS             8070..9092
                     /locus_tag="VKU03_10675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007689394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ71773.1"
                     /translation="MMKRQADSPRASRRYEMVEEVLRQNILGRQVPRGLVLLEGPIAA
                     LMQTSRAPVKTALRHLEAQGLVRRFNGRGFMVAKPGEPLDPVRRDLREIGLRIPKAID
                     DALQSRASWERIDETVEHDVAACLVFGRYRLIEAEIAEHFRVSRTVVRDVLGRLEERG
                     LVEKNQNSHWTAGPLSARSIREHFALRKILEPQALVTAAPLLERRRLGEIFARFRQAE
                     AAFPDSGGDGEGQELEAFETLLIDTCVLATPNERLREQIRRNLLPVLAAERVLRQLGL
                     PSDRTAITEHRLIVELLLQDAAPAAAAMLDAHLAAAERRNIAQLKIVAVLPEPESIAR
                     YLTRLD"
     gene            complement(9108..9584)
                     /locus_tag="VKU03_10680"
     CDS             complement(9108..9584)
                     /locus_tag="VKU03_10680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004117136.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family winged helix-turn-helix
                     transcriptional regulator"
                     /protein_id="HLJ71774.1"
                     /translation="MIRASASGARPSLGQIGLQQFVPYLINSISLSWTTHLADALKLQ
                     DMTTTKMRALAILSISSPVTINELSFYALTEQSTMSRTLDALEEQGLIVRKPRPEDLR
                     VRDVDITQAGRDAFAKVWPTMYDLLLKMLDGVDADEYKAFIATLHKIVRNIGNYGI"
     gene            complement(9633..11213)
                     /locus_tag="VKU03_10685"
     CDS             complement(9633..11213)
                     /locus_tag="VKU03_10685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010913234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLJ71775.1"
                     /translation="MAGADYVIVGGGVNGLVAAAMLGKKGRKVLLLDRNERVGGCLRS
                     EEITAPGFVHDVMATTLVLFLTSPAFAALGKDLQARGFAVAHSDLPTGVLRPDGSSVL
                     FSRDRARNIATFEACAKGDGEAYAQEMDRVGADAPFLFSLLGGSLWSRKMAATLAREA
                     WRRGPRGLAAFFGEALRPARAYLESTYASDAIHALWAPWGLHCGLNPESSYSAQMVKV
                     IAFAVELAGCPIATGGAKTLLSAFERLILDQGGEIRCGADVERVEIGAGGRADAVRLA
                     SGERIEARNGVICSTTPTQLYGRLLRDHPLPPDIARAVKQYRYGKGDMQIHYALDAPP
                     RWKGGEALGKVALLHVTPGLDGVSRAANECERGLLPAEPTICVGQPTALDPSRAPPGK
                     AILWLQLPEAPRVLKGDAAGEIAIPPEGRWTEAVREAFADRAEGLFARHIDDFRSHVI
                     ARKAYSPADLESMNVNLVGGDPYGGSCTIDQFFLWRPFAVSTNHRTFVPGLYHIGAST
                     HSGPGLSGGSGFLLASAL"
     gene            complement(11222..12010)
                     /locus_tag="VKU03_10690"
     CDS             complement(11222..12010)
                     /locus_tag="VKU03_10690"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLJ71776.1"
                     /translation="MSASRTLSDLASALLSGAVRVVDLSAPLGPETPIINLPPQVGKN
                     SPPVKVHSISKYDSDGPGWAWNWYELGEHTGTHFDAPRHWITGKDYSDGATDTIPPKR
                     FVAPANVIDRSKEAAANSDYLLSVDSIKEWEKEHGEIEAGTWVLLRTDWYKRNSSAKT
                     FLNADAAGTHYPGPTAEAIQYLVSKGVIGWGSEAVATEAGGAGGWNPPFPAHHYMHGA
                     NRCGLASLCRLDELPPKGAVLIAAPLKFVNGTGSPVRALALVPA"
     gene            12273..13838
                     /locus_tag="VKU03_10695"
     CDS             12273..13838
                     /locus_tag="VKU03_10695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997421.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLJ71777.1"
                     /translation="MAYDAIVIGAGHNGLAAALRLSEKGWRVAVIEAKDQPGGAVKTR
                     EATLPGFRHDLCAMNLSMFAGSPFFAAHKDLLHAHGLAFAPAEDCFASVFRDATFLGV
                     SKTLEKTVAGLSALSPADAQAWRDLLARFGGDAPHLFALLGSPMPSWGAAKVVWKAWR
                     EKGVSWLYDTLKMLLATPRDFLDAHFEHPKVKAMMAAWGLHLDFAPDIAGGALFPYLE
                     SMANQSFGMVIGRGGADAIVKAMVGALIAKGGELRLGERVSEIEVARGVASGVRLAGG
                     GRLAAKTIIANVHPKIVFGDLVSRAGPRAKYEGEIAKFRAGPGTMMVHLALDDLPAWR
                     AGDALRNYAYVHIAPDLEMMSRVYAEAAAGLLPAEPALVVGQPTAIDPSRAPAGKHVL
                     WVQVRVLPGQIRGDAAGRIAAVTWDDAKEAYAERALDLLESYAPNLRARILGRAVFSP
                     LDLERENPNLIGGDNLSGSHHLDQNFFFRPVAGWSRYRTPVKNLYLCGASTWPGAGTG
                     AGSGFMLARMLAG"
     gene            13891..14430
                     /locus_tag="VKU03_10700"
     CDS             13891..14430
                     /locus_tag="VKU03_10700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010659208.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ71778.1"
                     /translation="MLPVKTVASEQSTDNSEPSADFEILVRGAHSGQQPQLRLWLRML
                     SITKMISQEIRRRLRAEFDATLPQFDLLAQLYREPEGLRLSDLSRRTMVTNGNITGLA
                     DRLEADGLIVREMLNGDRRVTVAKLTRRGREIFAAMAKAHEGWLRELMADVDEETLAG
                     GLQHLAKIKGSASRRLVDD"
     gene            14533..>14843
                     /locus_tag="VKU03_10705"
     CDS             14533..>14843
                     /locus_tag="VKU03_10705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093350.1"
                     /note="catalyzes the conversion of salicylyl-CoA to
                     gentisyl-CoA; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional salicylyl-CoA
                     5-hydroxylase/oxidoreductase"
                     /protein_id="HLJ71779.1"
                     /translation="MRIVCVGGGPAGLYFGLLMKLRRPDHRVTVIERNKPYDTFGWGV
                     VFSDAMMEAMRAADPVSAAEIADAFNHWDDIELIFKGRRQRTTGHGFIGIGRKRLLNI
                     L"
BASE COUNT         2669 a         4804 c         4887 g         2483 t
ORIGIN      
        1 atgacgacgc ctgccgcttt cagcgtggcc agagagcctt caaaattagc cagaacttcg
       61 gtttcgcaat cgtgcagcac gacatttgtt ggaacgacga tacgaagacc gtggacgatc
      121 gccggcgccg gaagcgttgg cgtaaccccg cgcatcgccg catcgacgag gattgcgtcg
      181 gcgaccgcgc cggcgaagac ccccagcgta tcgagtgttt tggagagcgg gaaagcgccc
      241 gacattgggt agcggccagc cgaggctttg tagccgacga cgccggtgaa ggccgcagga
      301 atccgcaccg aaccgccggt gtcggtgccg atcgccgcgg gcgtcaggcc gcgcgcgacc
      361 gcgacggccg atccggacga cgatccgccg ggaacgcgcg gcgtcgatcc atacggattg
      421 cgcggcgtgc cgaaacgcgg attgaggccg atgccggaat aagcgaattc ggtcatgttg
      481 gtccgcccga ccgccgccat gccggcggcg gcgagcgcgg cgacgaccgg cgcgtcggcc
      541 ttggccggcg cggcgtcggc cagcgcgcgc gaaccggcgg tggtgacaaa gccggcaaag
      601 tcgaagaggt ccttccaggc gatcggcgcg ccgtcgagag ggccgagggc ccggccggcg
      661 gcgcgccggg cgtcggacgc ggcggcttcg cggcgcgcgc gctccgccgt gaccacggtg
      721 aaaattgccg gatcgctttc ctcgatcgcc ttcaacgtct gctcgacgag gcgccgcgca
      781 tcgatcgccc ccgcttcgag gcggcgggca aggtcgagca gaaccgaatc gggcgcgcgc
      841 gccatgtcag ccgcctcccg cggggatgac ggacgccgct tcggcgctcc atctgagcca
      901 gaccgcctgg tcgatggtca gcgtctcggc aaagcgatcg gcgtgaccct cgacgcggat
      961 catcgtcccg tcggcgagcg cgaggcgcaa agccgcgaga ttgccgctgc gctcgcggtt
     1021 ctcgattctc gcggcgatcc ggttgcccgg ctcgtccggc gccgcttcgc cgtcgcgccg
     1081 cggcgcgacg gcgaaagctt ccgacggcac gaccagcttg gccgcgccgg ccgcttcgcc
     1141 gacggcgcgc ccgctgaaca ggccgaagcg ggtcttgacc agcgcgatct cgccgcggcg
     1201 ctcgacgccg tcgccgtcga aaatgatgtt gcggccgatg aagcttgcga cgaacggcgt
     1261 ttgcggccgg cgatagagct cgtgcggcgg cgaaatctgc tcgatccggc cgcgattcat
     1321 cacgacgatc cgatcggaga gagccagcgc ctccgactgg gcgtgcgtga cgaagacgaa
     1381 ggtgacgccg agattgcgct gcagcaggcg caattcgccc tggatcgtct tgcgcagatt
     1441 ggcgtcgagc gcgccgagcg gctcgtcgag caggaggatg tcgggctcga tgacgaggct
     1501 ccgcgccagc gcgacgcgct gccgttggcc gccggaaaga cggtccgtgc ggctgtcgat
     1561 aatgtcggcg agcccgagct tctcgacgat ccgctcgacg cgctcgtcga tcgtcttgcg
     1621 gtcgagccgc ttcaccttga ggccataggc gatgttgccg cgcacggtca tgtgcgggaa
     1681 caaggcgtag ctctgaaaga tcattgcggt cgggcgcttc tcggcgggca gcgcgttgat
     1741 ccgcttgccg cggatgaaaa tatcgccgga cgtaatgatc tcgaaaccgg cgatcattcg
     1801 cagcgtcgtc gtcttgccgc agccggatgg gccaaggaag gcgacgaact cgcctttggc
     1861 gacatcgaga ttgaaatcga cgacggccgc cgcgccgttg tcgtaggtct tgccgacgcc
     1921 gacaagctga aggtcgcgct ccacgccgca aatccttaaa tccagggcgg cggcgcggcc
     1981 gcatcgccgc gccgccgacc gaacgctcag gcgttcagga actcgtccca tttctggatg
     2041 acgaaatcgt actcgtccgg ccattggtgc caataagcga catgggcggc gcgggtttcg
     2101 agcgagccgc cgtcgcgcag atcgccctcc ttgatgccgc gttcggcctt gcccttccaa
     2161 ggtttgcctt cgtaccagaa ggcgtatttg tccgggtcca tcgcctgctt gatgttcgtc
     2221 gaaggcgaat agtagccttg ctccgaaacc gtgatgcccg gcttgccgga cagccagaaa
     2281 tcggcatagg cgtagacggc ctctttgttc ggcgagccgg cgatcaaaga cgggccgatc
     2341 gcccaggcgc gatagccctc tttcggcacg gcgtatttgc agggcttgcc ctgcgccttg
     2401 accgccatga ccgcgggctg ccacgcgtcg cagacgacca tctcgccgga ggcgagcagg
     2461 ttcaccagct cgccgaaatc gccccataga gcgcggaact ggccgccctt tttcttcgag
     2521 atcaggaact tcatcgcttc cgcgatctcg gccttggacg cgttgcccgg attcttcacc
     2581 tgcgacatgc cgagcgtgtt catcgccatg atcgcctggc cgagcgcgat cagcggatcg
     2641 gtgttgaggc ccgattttcc tttccactta tcatcgaaaa tggctgtcca ggtattggcc
     2701 tcttccgccg tcacgacatc gggattgtag ccgatcgagt cgtaattata gacgacgggg
     2761 accatccaca gcgatttctc cgaatcgtcc gtccatatct ggccgacgat ctgcgccttg
     2821 cggtcccatt tcgggtccgg cttggtgaag gttgggcgaa tgttcgacca gttcttgatc
     2881 gccgaagccg ggatggcgtc gatattgttg gtctgcacga ccgccggcag ccgctcgccg
     2941 atcgtttccc agcaatcgta gtctttcgag ccggaaagga tcttggtttg cgcgtcgggg
     3001 aaggtcgcgg cggttccgga tgtggagccg acgccggatt cggccttgaa gtcggcgaga
     3061 atgcgctcct gcaccgtcac cgataagccg atcgttcgaa gcgtttcgcc gctcagcttg
     3121 ctcgccgccg cataggcgtt gcgggaatcg agcagcggcg tgccgccgcc gatcgccaaa
     3181 gccgccaccg cggcgccgga cttgagaagc gaccttctgg ataaatctgt ctcgctcatg
     3241 gatactccct ctccattcga cgcttacgaa ccctgacgat cctcagtagc gtccgacaag
     3301 aagaccgcca tcaaccacga ttgtttgccc cgtcatgtac cgcgccgcct tcgaggcgag
     3361 aaagtggatg acatcggcga cgtccgacgg ctcgccgatg cgccccatcg gaatgaaagc
     3421 gcccgccgcc tcggcgcctt ccgggccgag cgaatgctcc ttcgagagca attgggcggt
     3481 ccggatgtag ccgggcgcga cgccgttgac gcgaattcca gcgcgcgcca gctcgaccgc
     3541 caggccgcgc accagcccga cgacgcccga tttcgccgcc gaatagtgga catgctcgtc
     3601 ccagccataa gcgatgccca tgatcgacga gacgcagccg atcgctccgc cgcccttgcg
     3661 cttcatcgcc ggcagcgccg gccggatgac gcgcaacatg cctttcaagt cgatctcgaa
     3721 cgtatggtcc catatctcgt cggtgagatc ggcgagcggc gtgcgatgag cgatgccggc
     3781 attggcgaca accacgtcga tcgcgccgcg tcgcgcttcg atctccgcga cgattttgtc
     3841 ggcctgccgc gtcgagcgga cgtccaaggc gatgaattcg gccgaaccgc ctttggcctc
     3901 ggcctcttgg gcggcgcttg agccttcggt ttcgagaatg tcggtcacga cgacatggtc
     3961 gcccgcggcg gcgaaggcga gcgcggtcgc gcggccaatg ccgatgccgc cgccggtgat
     4021 cagaatcgtc ctctggccca tgcctcgccc ctcacagcat cacgtcgccg gaattcggcc
     4081 cgagcgtctg accgacgaaa aggcccgctt catcggaggc gaggaacgcc acggcggcgg
     4141 cgacctcttc cggttggccg aaccggccga gcggcagctc cgccgccttg cgccgccgcc
     4201 aatcctccga caaagccatg acgagcggcg tttcgatcgg gcctggcgcg accgcgttga
     4261 cgcgcacgcc atgcggactg aactcgcgcg ccagcgactt ggtcaggccg atgatcgcgg
     4321 ccttggcggc gctgtaatgg gcgagctcga cgccgccgat ctggccgagc tgcgacgcga
     4381 tgttgacgat gacgccggat ttcgccgcca gcatcgccgg caagacggcg cgggcgcaga
     4441 gataggcgcc gcgcacatgc acggcgaaga tgcgatcgaa atccgccggc gcgatgtcga
     4501 gaaacggcgc ctgatggaca tggccggcgc aattgacgag aagcgtggcg tcgccgaatg
     4561 cggccgcgcc ggcggcgacg atcgtcttca cgtcggcttc gctcgcgaca tcgccgccga
     4621 ctgcgatcgc gcggccgtcc gccgccgcga tctccgccgc gaccgcctcg gcccgattct
     4681 tggcgacatc gttgaccgtc accgccgccc cgtcggcggc gagccggcgc gcgatcgccc
     4741 ggccgatgcc ggagccgccg ccggtgacga ttgcgatggt cttctggccg gcgcgtctca
     4801 tgcttcttcc tgaatcatga gcctgcgggc gcgcctgacg ctcagcgcca taaggatcgc
     4861 gtagatcgcg agcagcgcga acgagaacag agtggtgagg acgccgaacg cgaagatgtt
     4921 cgggtggatt tcgaccgaga acgtgccgta gatcgcgagc ggcagcgtca attcgcggcc
     4981 ggacgtgaac agcgtgcgcg agaattcatc ataggagagg gtgaaggcga acagcatggc
     5041 cgagagcacg ccgggaaaga tcagcggaaa agttactttg cgaaaggtgc gggccggcga
     5101 gacgccgagc gaccacgagg cttcttcgac gctggcgtcg aaccggttga agatcgccag
     5161 catgacgagg aaggcgaagg gaaacgtata gacgacgtgc agcgcgaaag cggtgctcca
     5221 ccaatgccgg tccaagccga tcgtgttcgc gagcagggcc atgccgaggc cgatcagcac
     5281 gccgggcacc atcatgccga ggatgatcag atagaagacg agtccggcgc ccttgaacct
     5341 ggttcggaac gcctgggccg ccatgacgcc gaggacggtc gagacgatca tcgtcatgac
     5401 cgccagcgtc agtgagcgcg ccagcgcgct ctcgatcggc agcggcgcga tgcgcgaggg
     5461 cggcgtgagg ccgaagagat gcttgtacca atagaccgac caggcgacga tcggaaattg
     5521 cggcccgcct tccggcccgg tctggaagga taggatcgcc agaacggcga agggagcata
     5581 aagaaaaacg acgataagcg ccgtaaagcc gccaagcgcc catttgatca ggcggctctc
     5641 catgtcagag ctcccgcctg aggtcgacga cgcgcagaag gatgctgacc gccgcgccca
     5701 tgatgatggt cagaacgacg ccgaccaccg cggcgaaaga ccaccggagc gagccgacct
     5761 gggtgacgat gatgttgccg agcagattga ccttccgccc cgacaaagcc gccgacgtcg
     5821 cgaactcgcc gagcaccatt accgagacga agatcgcgcc gacgaccacg ccaggcatgg
     5881 agagcgggag gatgatcgtc cggaatatcc gccagaagcc ggcgccgagg tcgcgcgcgg
     5941 cctcgatgat cgtcgggtcg atccgactca gcatgaaagc gatcgggccg accatgaaga
     6001 cgcaatagat ctgcgtcatg ccgatgatca ccgaaaggtc ggtatagagc agcgcctcga
     6061 tcggctgcga gatcaggccg gctttcatga gaaggatatt gaccgcccct tccgtgccga
     6121 gcatgggccg ccaggcgagg acgcggatga ggaaggacgt ccaaaacgga atgacgcaga
     6181 cgacgagaag ggccgtctgc aacgtcttgt tgcgaacgaa aaggccgatg aacaaggccg
     6241 tcggatagcc gaccagcagc gtcgcgccga gcacgatcag ccagatgcgg atcgtcgtcc
     6301 acagcgcgtc gagataggtt tccgaggtga agaaagcgac ccaactcctg gtcgtcagaa
     6361 tcggctcgat cttgaacgtc gtctgcgtcc agaacgaaac atagatcatc atcgccatcg
     6421 gcaggacgag gaaaatcagc atccaaagga cgccgggggc gatcagccaa tagacgacgc
     6481 gccgccgcgt ttgctgcgcc gccgcggcgg gcgattcgat cgcgtctgaa actatcgcgg
     6541 tggtcaatgc gtcctctgga cctaaaccgg ttcgaccttg gctcaagcga aaacccgcgc
     6601 gccgtcgcgg cgccatgaaa gggcgtaagc ttcgccgagt tcgtattgcg gcggggcgcg
     6661 atcgccgagg ggcgtctcca cttcgacgat ttggccgctc gccagcttga agaacgaaag
     6721 gagagaaggg ccggtaaatt cgctcgcgac aaaagcggcg gtcaggctga cctgatcggc
     6781 gccgcttttc tccgcccgcg gcgccaccgc gatccggtcg tagcgcacgc cgtaaaaccc
     6841 ccgcggcggg caggggccgc gcggcagcgg aaactcgccg gcgtcgcttt ggaacgaagc
     6901 gccggcgacc gcgccgccaa acaaattgta gcagttgagg aatcgcgcga cccgcgccga
     6961 ggccggccgt tcgaacagcg tatcgggatc gtcaaactgg acaacgcggc cgcgatcgag
     7021 cacgatcacc ctgtcgccga tcgcaagagc ctcgctctcg ctccctgtga catgcaggaa
     7081 ggtgacgccc aggcgctcgc ggatcatccg caattcggcg cggatgcgcg tgcgcagatt
     7141 ggcgtcgagc gcgccgagcg gctcgtcgag caggacgagc ggcggctcgg tgaccagggt
     7201 tcgcgccagc gcgacgcgct ggcgctggcc gccggaaatc tgatggacgc cgcgcgcttc
     7261 gagtccggca aggccgacaa gctcgatcat cgctcggacg cggtcgcgga tttcgctttt
     7321 gccgagcgtc gcgccggcgc ggtttttcaa tccgaaggcg acgttttcct cgaccgacag
     7381 gtgaggaaag agcgcaaagt tctgaaagac gaagccgatc ccgcggttat agacgggcgc
     7441 tgcgacgatc ggccggccgg caaattcaat cgtccctgcg tcgggctctt cgaagccggc
     7501 gatgacgcgc aacagaaccg tcttgcccga gccgctcggg ccgaggagcg agacatagca
     7561 atctcgcgga atatcgaggc tgacgtcgtc cagcgcgacc gcgccgccat agcgcttggt
     7621 caggccggtt atctgcagca ctccagcact caccagcgcg ccctcgcctc cgccgccgct
     7681 tcaccgcatc ggcgaccgat cccttcccat gccgacacct tacaatattg ccttttgtgt
     7741 tcaagtccat ttttgatccg cccgtttaat ttctgacggc cggcagaccg gccgcttctc
     7801 atattggcgc gcaggcggcg tcatcgaaca aaaatacccg gattttggcg ggatatgact
     7861 cggcgaccac acggcgcgct acgggcctcg cggcgtctcc tcagggcggc ggcccgcatt
     7921 tgacgcccaa ttcctttctc gtccttcgat tgaaagcggc aaaatcgagc gttataaaat
     7981 ggccacggtg ctcaaagtcc attcagtggc ggggccgttt cagccttccc ggagatcgac
     8041 tacaaggcgg agaacaagac ttccgacgga tgatgaaacg acaagccgat tcgccgcgcg
     8101 cgtcgcgccg ctacgagatg gtcgaggaag ttctccggca gaacattctc ggccggcagg
     8161 tgccgcgcgg cctggttctg ctggaaggtc cgatcgccgc tttgatgcag acgagccgcg
     8221 cgccggtgaa gacggctttg cggcatctcg aagcgcaggg gctggtgcgc cgcttcaacg
     8281 gccgcggctt catggtggcg aagccgggcg agccgctcga cccggtccgg cgcgatctgc
     8341 gcgaaatcgg gcttcgcatt cccaaggcga tcgatgacgc gctgcagagc cgcgcatcgt
     8401 gggagcgcat cgacgagacc gtcgagcacg atgtcgccgc ctgcctggtc ttcggccgct
     8461 accgcctgat cgaggcggag atcgccgagc atttccgcgt cagccgcacc gtcgtgcgcg
     8521 acgtgctcgg ccggctcgaa gagcgcggcc tggtcgaaaa gaatcagaat tcgcactgga
     8581 ccgccggccc gttgagcgcg cgctcgatcc gcgagcattt cgctttgcgc aaaatcctcg
     8641 agcctcaggc gctcgttacc gccgcgccgc tgctcgagcg tcgccggctc ggcgaaatct
     8701 tcgcgcgctt ccgccaggcg gaagccgctt tccccgattc cggcggcgac ggcgaaggac
     8761 aggagttgga agcgttcgaa acgctgttga tcgacacttg cgtgctcgcc acgccgaacg
     8821 agcgcttacg cgagcaaatc cgccgcaatc ttctgccggt gctcgccgcc gagcgggtgc
     8881 tgcgccagct cggcctgccg agcgaccgga ccgcgatcac cgagcatcgc ctgatcgtcg
     8941 aattgctgct tcaggacgcc gctccggccg ccgccgcgat gctcgacgcg catcttgccg
     9001 ccgcggagcg ccgcaatatc gcgcagctca agatcgttgc cgttctgccg gagccggaat
     9061 cgatcgcccg ctacctgacg cggctcgact gaccgctaca acgaacctca aatgccgtag
     9121 ttgccgatat tgcggacgat cttgtgcagc gtggcgatga aagccttgta ttcatcggcg
     9181 tcgacgccgt cgagcatctt gagcaagaga tcgtacattg tcggccagac tttggcgaag
     9241 gcgtcgcggc cggcttgggt gatgtcaacg tcgcgcacgc gcagatcttc cggccgcggc
     9301 ttgcgcacga tcagcccttg ctcctccagc gcgtcgagcg tccggctcat cgtcgactgc
     9361 tcggtcaagg cgtagaagga aagctcgttg atggtgaccg gggacgagat gctgaggatg
     9421 gccagggcgc gcatcttcgt cgtcgtcata tcctgcaatt tcagcgcgtc ggcgagatgc
     9481 gtcgtccagc tcagcgagat gctgttgatc aaatagggca cgaattgctg cagcccgatt
     9541 tggccgagcg agggccgcgc gccggaagcg ctcgcgcgga tcataacggg atcgtcgaac
     9601 aaggcggcgg gcgaattcgt ctggtcggtc atttaaagcg cgctggcgag aaggaagccc
     9661 gagccgccgc tcaggccggg cccggaatgg gtcgacgccc cgatatgata gaggccggga
     9721 acgaaggttc tgtgattagt cgaaaccgcg aacgggcgcc agagaaagaa ttggtcgatt
     9781 gtgcaggagc cgccataagg atcgccgccg acgagattga cgttcatgct ctcgagatcg
     9841 gcgggagaat aggccttgcg ggcgatcaca tggctgcgga aatcgtcaat gtggcgcgcg
     9901 aacagcccct ctgcgcgatc ggcgaacgcc tcgcgcacgg cctcggtcca gcggccttcc
     9961 gggggaatcg cgatttcgcc cgccgcgtcg cctttcaaga ctcgcggcgc ttcgggcaat
    10021 tgcagccata gaatggcttt gcccggcggg gcgcgcgacg ggtcaagcgc cgtcggctgg
    10081 ccgacgcaga tggtcggctc cgccggcagc aggccgcgct cgcattcatt ggcggcgcgc
    10141 gagacgccgt cgaggccggg cgtcacgtgc agcagcgcga ccttgcccaa cgcctcgccg
    10201 cctttccagc gcggcggcgc gtcgagcgcg taatggattt gcatatcgcc cttgccgtag
    10261 cgatattgct tcaccgcgcg cgcgatgtcc ggcggcaggg ggtggtcgcg cagcaagcgt
    10321 ccatagagct gcgtgggagt cgtcgaacaa atgacgccat tcctcgcctc gattcgttcg
    10381 cccgaagcga ggcgcaccgc gtcggcgcgg ccgccggcgc cgatctcgac gcgctcgaca
    10441 tccgcgccgc accggatttc gccgccttga tcgaggatca ggcgctcgaa tgcggaaagc
    10501 agcgtcttcg ccccgccggt cgcgatcggg caacccgcga gctccaccgc gaaggcgatc
    10561 accttgacca tctgcgcgga gtacgaactc tccggattga ggccgcaatg aaggccccag
    10621 ggcgcccaaa gcgcgtggat cgcatccgag gcgtaggtcg attccagata ggcgcgcgca
    10681 gggcgcagcg cttcgccgaa aaacgcggcg agcccgcgcg gaccgcgcct ccaggcttcc
    10741 cgcgcaagag tcgccgccat tttccgcgac caaagcgaac cgccgagcag cgagaacaga
    10801 aatggcgcgt ccgcgcccac ccgatccatc tcctgggcgt aagcctcgcc atcgcctttc
    10861 gcgcaagctt cgaacgtcgc gatgttgcgc gcgcgatcgc gggagaacag cacggaagat
    10921 ccgtccgggc gcagcacgcc ggtcggcagg tcgctatgcg cgacggcgaa gccgcgcgcc
    10981 tgcaggtcct tgccgagcgc cgcgaaggcc ggcgaggtca ggaagaggac gagcgtcgtc
    11041 gccattacgt cgtggacgaa gccgggcgcg gtgatctcct cgctgcgcaa gcagccgccg
    11101 acgcgttcgt tgcggtcaag caggagaacc ttgcggccct tcttgccgag catcgcggcg
    11161 gcgacgagcc cgttgacgcc gccgccgacg atcacgtagt cggcgcccgc catgcctcgt
    11221 ctcaagccgg gacgagggcg agcgcgcgca caggcgagcc ggtgccgtta acgaatttga
    11281 gcggcgctgc aatcagcact gcgcccttgg gcggcagttc gtcgagacgg cagaggctcg
    11341 cgagtccaca gcgattggcg ccgtgcatgt aatgatgcgc aggaaacggc ggattccaac
    11401 cgccggcccc tccggcttcc gtggcgaccg cctctgaacc ccaaccaatt acgcctttgg
    11461 aaacgagata ctgtatagct tccgcagttg gacctggata gtgagtaccg gcggcgtcgg
    11521 cgttgaggaa agtcttcgcg ctcgaattgc gcttgtacca atcggtgcgc agcagcaccc
    11581 acgttccagc ctcgatctcg ccgtgctcct tctcccattc cttgatgctg tcgacgctta
    11641 gcagatagtc gctgttcgcc gctgcctcct tcgagcggtc gatcacgttg gcgggcgcaa
    11701 caaacctctt cggtgggatc gtgtccgttg cgccgtccga ataatccttg ccggtgatcc
    11761 aatggcgggg cgcgtcgaaa tgcgtgcccg tatgctcgcc gagttcgtac cagttccagg
    11821 cccagcccgg gccgtcggaa tcatatttcg agatcgagtg caccttgact ggcggcgagt
    11881 tcttacccac ctgcggcggc agattgatga tcggggtctc tggacccagc ggcgcggaaa
    11941 ggtcgacgac ccgcacggcc cccgatagaa gcgccgaagc gagatcagaa agcgtccggc
    12001 ttgcactcat aaggcgtcct ccgatatcgc gcgcccgctc tttcgaacga cccgtcccca
    12061 gcggcgcgag tcgcgaagac taggcggaga aatatgaatt tgcaacaatc ccgggcaagc
    12121 gcggagcgcc cggcgtcgcc gcggatattg tcgatcgccg cgcaataagc caaaatcgac
    12181 atgatagagg cctcgatcga gataggcggc tggagcgacg ttcatgccag ggcgccaatt
    12241 gcttggatat gcatggaatt gcggagatgc ccatggcgta tgacgcgatc gtaatcggcg
    12301 cggggcacaa cggcttggcg gcggcgttgc ggctctcgga aaaaggatgg cgcgtcgctg
    12361 tgatcgaggc gaaggaccag cccggcggcg cggtgaagac gcgcgaagcc accctgccgg
    12421 gcttccgcca cgatctttgc gcgatgaacc tttcgatgtt cgccggctcg ccgttcttcg
    12481 ccgcgcataa ggatttgctc cacgcgcacg ggctggcctt cgcgccggcc gaagattgct
    12541 tcgccagcgt gtttcgcgac gcgacctttc tcggcgtcag caaaacactg gagaagactg
    12601 tcgccggctt atcggcgcta tcgccggccg acgcgcaagc ctggcgagat ttgctcgccc
    12661 gcttcggcgg cgacgcgccg catcttttcg cgctgctcgg ctcgccgatg ccgtcctggg
    12721 gcgcggccaa ggtcgtctgg aaggcatggc gcgaaaaggg cgtatcctgg ctgtacgata
    12781 cattgaagat gctgctggcg acgccgcgcg actttctcga cgcgcatttc gagcatccaa
    12841 aagtcaaggc gatgatggcg gcctggggcc tgcatctcga cttcgcgccc gatatcgcgg
    12901 gcggggccct gtttccctac ctcgaatcca tggccaacca gagtttcggc atggtgatcg
    12961 gccggggcgg cgccgacgcc atcgtcaagg cgatggtcgg cgcgctcata gccaagggcg
    13021 gcgaattgcg gctcggcgaa cgcgtctccg agatcgaggt cgcccgcggc gtcgcgtccg
    13081 gcgttaggct tgccggcgga ggccggctcg cggcgaaaac catcatcgcc aatgttcacc
    13141 ccaagatcgt gttcggcgat ctcgtttctc gcgccgggcc acgcgcgaaa tacgaaggcg
    13201 aaatcgccaa gtttcgcgcc gggccaggca cgatgatggt tcatctggcg ttggacgacc
    13261 tgccggcctg gcgcgccggc gacgctttgc gcaactacgc ctatgtgcat atcgcgcccg
    13321 acctcgaaat gatgtcgcgc gtctatgccg aggccgcggc gggattgctg ccggccgagc
    13381 ccgcgctcgt cgtcggccag ccgaccgcga tcgatccttc gcgcgcgccg gcgggaaagc
    13441 acgtcctttg ggtgcaggtg cgggtgctgc ccggacaaat tcgcggcgac gcggcgggcc
    13501 ggatcgccgc cgtgacctgg gacgatgcga aggaggctta cgccgagcgc gccctcgatc
    13561 ttctcgagag ctacgcgcca aatcttcgcg cgcgcatcct cggccgggcg gtcttttcgc
    13621 cgctcgatct cgaacgggag aatcccaatc tgatcggcgg cgacaatctc tccgggagcc
    13681 accatctcga ccagaatttc ttctttcgcc cggtcgcggg ctggtcgcgc taccgtacgc
    13741 cggtgaagaa tctctatctt tgcggcgcct cgacctggcc gggcgcggga accggagcag
    13801 gatcgggctt catgctggcg cggatgctgg cagggtgagc gcgatctttg acatttaaag
    13861 catcctgtcc tatggcgggc gccatcctcc gtgcttccgg tcaagaccgt ggcgagcgag
    13921 caatccaccg acaattccga gccgtccgcg gatttcgaga ttctcgtccg cggcgcgcat
    13981 agcgggcagc agccgcaatt gcggctatgg ctgcgcatgc tgtcgatcac caaaatgatc
    14041 tcgcaggaaa tccggcgccg cctcagggcg gagttcgatg cgacgctgcc gcaattcgac
    14101 cttctcgccc aactctatcg cgagccggaa ggcctgcggc tcagcgactt gtcgcgccgg
    14161 acgatggtga ccaacggcaa cattaccggt cttgccgacc ggctcgaagc cgatgggctg
    14221 atcgtccgcg aaatgctcaa cggcgaccgg cgggttactg tggcgaaact gacgcgacgc
    14281 ggccgcgaga ttttcgccgc catggccaaa gcccatgagg gctggctgcg cgagttgatg
    14341 gcggatgtcg acgaagagac cctggctggc ggcctgcagc atctggccaa aatcaaaggc
    14401 tcggccagcc ggaggctggt cgacgactag ccacgaaacg cgcgaatgcg tcgcggcccg
    14461 cggggcttga ccttgcgcgc cgagatattt taagcttgaa gcaattagac tcggatcgag
    14521 gagagccgag gcatgcggat cgtttgcgtc ggcggcgggc cggccgggct gtatttcggg
    14581 ttgctgatga agctgcgccg ccccgaccat cgggtcacgg tgatcgagcg caacaaaccc
    14641 tacgacacgt tcggctgggg cgtcgtcttt tcggacgcga tgatggaggc gatgcgcgcc
    14701 gccgatcccg tcagcgccgc cgagatcgcc gacgccttca atcactggga cgatatcgag
    14761 ctgatcttca agggccgccg gcagcgcacg accggccacg gcttcatcgg catcggccgc
    14821 aagcgccttc tgaacatcct tca
//
LOCUS       DATGJW010000350         2382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_379670, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000350 DATGJW010000000
VERSION     DATGJW010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2382
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_379670"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..450)
                     /locus_tag="VKU03_10710"
     CDS             complement(<1..450)
                     /locus_tag="VKU03_10710"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ71780.1"
                     /translation="MARLVFRRTIAALAGLAAAVTASAAFAGARIAVITPYLAQPGTQ
                     FYVEAFQDQAKANGWEVNVIDTKGDVAAAISRIEDVSNQKVDAIVINVDPAQVAAGLQ
                     TAKKAGVPVFGMDAGADPLLVTNVTSNGYSMAADTAAYVANRINGKGS"
     gene            697..2151
                     /locus_tag="VKU03_10715"
     CDS             697..2151
                     /locus_tag="VKU03_10715"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_function: GO:0016773 - phosphotransferase activity,
                     alcohol group as acceptor [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLJ71781.1"
                     /translation="MSPPFLIGVDVGTTSVKAALFDAAGQTLKTYASAYPTARPLPGW
                     AEQEPADWTSRVCAAIGQLVDGLPSGAVAGLGLCSQVNTHVFVDEKGEPLLPAIIWQD
                     GRCALEAAELDRLVAAKDRLEWWGAPLPIDASHVLSRMAFVARAHPEIWRKTRWVMAP
                     KDYCLFKLTGEVAADPMASFGVIDQSLAYIPSLIDLVPGAFERLPPLQGITARLGGVR
                     NGLPGAGLAVVNSTMDAWAGMLGAGAARQGDAAYLSGTSEVGGIVSARRIPTPGVITF
                     PSCEGITLHAAPTQAGGASVAWLAAILGRSADEISALAAKSALAQAAPIFLPHLQGER
                     APLWDINARASFSGLDGTMGAPEMARAVLEGVAYSVRLLMEALEKSSGVKPERLHHAG
                     GGAGSDVWCQIRADVLGRVIERKANRDAGVVGAAILAGIGTGVFGSISEAARHLARTE
                     REYTPNPARRAMYAEGFARYVELYGRLKGFCTGG"
BASE COUNT          400 a          753 c          810 g          419 t
ORIGIN      
        1 actgcccttg ccgttgatcc ggttggcgac ataggcggcg gtgtcggcgg ccatggaata
       61 gccgttgctg gtgacattgg tgacgagaag cggatcggcc cccgcatcca tgccgaagac
      121 cggaacgcca gcctttttgg cggtctgcag gccagcggcg acctgagcgg gatcgacatt
      181 gataacgatc gcgtcgacct tttggttcga gacatcctcg atgcggctga tcgcggcggc
      241 gacgtcgccc ttggtgtcga tgacattgac ctcccagcca ttcgccttgg cctgatcctg
      301 gaacgcctcg acatagaatt gcgtgcccgg ctgggcgaga tagggcgtga tcaccgcgat
      361 cctggcgccg gcgaaagccg cggacgcggt gacggcggcg gcaaggccgg cgagagctgc
      421 gattgttcga cgaaagacga ggcgcgccaa tgtttccccc cgggcgaaat tgcggaacaa
      481 atgttcggcc ggaagggtag catcgggcgg gggaagcgca aatcgtggga ggtgaggcgc
      541 gatatcctcc cccctgcagc gaagcggaac ggggagcaag accatgcgaa gcatggtggt
      601 cgggccccgc ggcggttgcg tctaatcttc aaggccgcca tacgcgccgt cccccgcgcc
      661 cgcttcgcag gggaggatga gtgctatcat cccggcatgt cccctccctt cctcatcggc
      721 gtcgatgtcg gcacaacctc ggtcaaggcg gcgctcttcg acgccgcagg ccagactctc
      781 aagacttacg ccagcgccta tccgaccgcg cggccgctgc ccggctgggc cgagcaggaa
      841 ccggctgact ggacctcgcg cgtctgcgcc gcgatcggcc agcttgtcga cggtttgccg
      901 agcggcgccg tcgcggggct gggcctttgt tcgcaggtca atacccatgt cttcgtcgac
      961 gagaagggcg agccgctcct gcccgccatc atctggcagg acgggcgctg cgctttggag
     1021 gcggcggaac tcgatcgtct cgtcgcggcg aaggaccgcc tcgaatggtg gggcgcgcca
     1081 ttgccgatcg atgcgagcca tgtccttagc cgcatggctt ttgtcgcgcg cgcccatccg
     1141 gaaatctggc gcaagacgcg ctgggtgatg gcgccgaagg attattgtct cttcaaactg
     1201 accggcgaag tcgcggccga tccgatggct tccttcggcg tcatcgatca gagccttgcg
     1261 tacataccgt ctctgatcga tctcgtaccg ggcgctttcg aacgcttgcc gccgttgcaa
     1321 gggatcacgg cgcggctcgg cggcgttcgc aacggcctcc ctggcgccgg cctcgcagtc
     1381 gtcaatagta cgatggacgc ctgggcgggc atgctgggcg cgggcgcggc gcgccaaggc
     1441 gatgcggcct atctcagcgg cacgagcgaa gtcggcggca ttgtctccgc gcgcagaatt
     1501 ccaacgcccg gcgtcattac ttttccttcc tgcgaaggga taacgctgca tgcggcgccg
     1561 acccaggccg gcggcgcctc tgtcgcctgg ctcgccgcga tcctcggcag gagcgccgac
     1621 gaaatatccg cactggccgc caagagcgcc ctcgcccaag cggcgccgat cttcctgccg
     1681 cacctgcaag gcgaacgggc gccgctgtgg gacatcaacg cccgcgcctc cttctccggc
     1741 ctcgacggaa cgatgggcgc gccggagatg gcgcgggccg tgcttgaagg cgtggcctat
     1801 tcggtgcggc tgctgatgga ggcgctcgaa aagtcgtcag gcgtcaagcc cgagcgcctg
     1861 catcacgccg gcggcggcgc gggttcggac gtgtggtgtc agatccgagc ggacgtgctg
     1921 ggccgcgtca tcgagcgcaa agccaatcgc gacgccggcg tggtcggcgc ggcgatcctc
     1981 gccggaattg ggacaggcgt gttcgggtcg atcagcgaag cggcgcggca tttggcgcga
     2041 accgaacgag aatatacgcc gaatccggcg cggcgggcga tgtatgccga agggtttgcg
     2101 cggtacgtgg aactgtatgg gcggttgaag gggttttgca caggcggata atcgagatcc
     2161 gccgcctaaa gcgcttggac gctcgggcag atccgccaag ccaatccggc aaggcgatcg
     2221 ccggatttgc ttcgacatgt aatccacatg tttctatgcc ctcaacatgg gaagaatggt
     2281 tcaaatccgc aacgtgcccg aggcgctgca tcgccagctc aagtcgcgcg cagctttggc
     2341 cggcatgtcg ctatccgact atctgctaga cgagattcgt ca
//
LOCUS       DATGJW010000351         3007 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_584364, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000351 DATGJW010000000
VERSION     DATGJW010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3007)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3007)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3007
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_584364"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..931
                     /locus_tag="VKU03_10720"
     CDS             <1..931
                     /locus_tag="VKU03_10720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2090 domain-containing protein"
                     /protein_id="HLJ71782.1"
                     /translation="GSPHRALRYDEALTHIHWATTRRPQPESLMAFAIDHRAQLEKIA
                     DAAKAPRARISAFKRLAVDAAANVAAGRSGFGMLLDGKYGREALFRAADHNFWIGRPV
                     EEPGSRPLDFEYGGSLGAKLVEWPVGHTIKCLCFYHPDDPDEMKARQERELLRLTDAA
                     RRIGRELLIEIIAGKHGAVSDTTVARVLERLYNIGIKPDWWKLEAQASGAAWENIARA
                     IDAHDPFCRGIVLLGLEAPEEDLASAFRIARRCGRVKGFAVGRTIFAEPAQAWFAGEI
                     GDDEAVRLMTDSFARLCEAWRDAEAAAPAGKVA"
     gene            928..2001
                     /locus_tag="VKU03_10725"
     CDS             928..2001
                     /locus_tag="VKU03_10725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011474543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71783.1"
                     /translation="MIDARDLDAALAAGVIDAATRDRLAAFLAARRPAPALAAPARKF
                     DVTHVLYYAGALLVMSAMGLFSNAAFNALGGYALTATALIYAAGFVALGASLWRKPDL
                     RTPAGLSIAVAVAMTPLAVYGVQDALNLWAADKPKDYQSFFPLINASWVYMEAATVAA
                     ALIALRYFPFPFIVMLAAIALWFMSMDLAALLARLYAPKDWLSDWELRRHVSLYFGVA
                     MILAAWALDLKRPAAGDFGFWLHLFGALAFWGAISASDEAAAIGKAMYCGLNVGLIGF
                     AIFLNRRIYAVLGAFGVAGYLGYLALDVFKDVLAFSFVVSLIGLGVIFAGVFYQRKRE
                     KISAWIDAMLPPAFRALRPQHSI"
     gene            2085..>3007
                     /locus_tag="VKU03_10730"
     CDS             2085..>3007
                     /locus_tag="VKU03_10730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018323768.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-binding protein"
                     /protein_id="HLJ71784.1"
                     /translation="MTEVDGEKVRLFAGVFAIFGHGNVARLGEALQAAQAALPTYRAH
                     NEQAMGLSAVAYAKAMRRRRVMACTSSIGPGATNMVTAAAVAHVNRLPVLFLPGDVFA
                     NRRPDPVLQQVEDFGDGTVSANDCFKPVSRYFDRITRPEQIIPALTRAVAVLTDPAEC
                     GPVTLAFCQDVQTEAYDYPETFFDERMHWRRRQGPDPHELREAAALLRGARKPLVVAG
                     GGVLYSGAEKELLAFCEKRGVPVAETQAGKSAVPADHPLALGAIGVTGTSAANAYAEE
                     ADAILAVGARLADFVTGSWALFKNPERRFVG"
BASE COUNT          436 a         1017 c         1010 g          544 t
ORIGIN      
        1 cggctcgccg catcgcgcgc tccgttatga cgaggcttta acgcacatcc attgggcgac
       61 gacgcgccgg ccgcagccgg agagcctgat ggccttcgcc atcgatcatc gcgcccagct
      121 ggagaaaatc gccgatgcgg cgaaagcgcc gcgcgcccgg atcagcgcct tcaagcgtct
      181 ggcggtggat gcggcggcca atgtcgcggc gggacggtcc ggcttcggca tgctgctcga
      241 cggcaaatat ggccgcgagg cgttgtttcg cgccgccgac cacaatttct ggataggccg
      301 gccggtcgag gagcccgggt cgcggccgct cgatttcgaa tatggcggct cgcttggcgc
      361 gaagctggtc gaatggccgg tcggccatac gatcaagtgc ctgtgcttct accatcccga
      421 cgatccggac gagatgaagg cgcgccagga gcgcgaattg ctgcgcctca ccgacgcggc
      481 gcgccgcatc ggccgcgagt tgctgatcga gatcatcgcc ggcaaacatg gcgcggtttc
      541 cgatacgacc gtcgcgcgcg tgctggagcg gctctacaac atcggaatca agccggattg
      601 gtggaagctc gaagcccaag cgagcggcgc ggcttgggag aatatcgccc gggccatcga
      661 cgcgcacgat ccgttctgcc gcgggatcgt cctgctcggc ttggaggcgc ccgaggaaga
      721 tttggcgagc gcgttccgca tcgcccggcg ctgcggccgc gtcaaaggct tcgcggtcgg
      781 ccgcacgatc ttcgccgagc cggcgcaagc ctggttcgcc ggcgagatcg gcgacgatga
      841 agccgtgcgg ttgatgacgg acagttttgc gagactctgc gaggcttggc gtgacgccga
      901 ggcggcggcg cccgccggaa aggtcgcatg atcgacgcga gggacttgga cgcggctttg
      961 gccgccggcg tgatcgacgc cgcgacgcgc gaccgcctcg ccgcgttcct tgccgcacgc
     1021 cggcccgcgc cggcgctcgc ggcgcccgcg cgcaagttcg acgtcacgca tgtcctctac
     1081 tatgccggcg ccttgctggt catgtcggcg atgggcctgt tctccaacgc ggcgttcaac
     1141 gcgcttggcg gctatgcgct gaccgcgacc gcgctcatct acgccgccgg cttcgtcgcg
     1201 ctcggcgcct ctctgtggcg aaagcccgat ttgcgcacgc ccgccgggct ctcgatcgcc
     1261 gtcgccgtcg cgatgacgcc cttggcggtc tacggcgttc aggacgcgct caatctctgg
     1321 gcggcggaca agccgaagga ttatcagagc ttctttccgc tcatcaatgc gagctgggtc
     1381 tatatggagg cggcgaccgt cgcagccgcg ctgatcgcgc tgcgctactt tcccttcccc
     1441 ttcatcgtga tgctcgcggc gatcgcgctc tggttcatgt cgatggatct cgcggcgctg
     1501 ctcgccaggc tctacgcgcc gaaagattgg ctctccgatt gggagttgcg gcgccacgtc
     1561 tcgctctatt tcggcgtcgc gatgatcctc gccgcttggg cgcttgacct aaagcggccc
     1621 gccgccggcg atttcggctt ctggctgcat ctgttcggcg cgctcgcttt ttggggcgcg
     1681 atcagcgcct ccgacgaggc ggcggcgatc ggcaaggcga tgtactgcgg gctcaacgtc
     1741 ggcttgatcg gtttcgcgat ctttctcaat cgccgcatct acgccgtgct cggagccttc
     1801 ggcgtcgccg gctatctcgg ctatctcgcc ttggacgtct tcaaggacgt gctcgctttc
     1861 tccttcgtcg tgtcgctgat cggcctcggc gtgatcttcg ccggcgtatt ctaccagcgc
     1921 aagcgcgaga aaatcagcgc ctggatcgac gccatgctgc cgcccgcctt ccgggcgctg
     1981 cgtccgcagc attcgatttg aggactggag gcaacggagg aggccccgtg aaaaccgtcc
     2041 gtctcaccgc agcgcaagcg accgtgcgct tcttgagcca gcagatgacc gaggtcgacg
     2101 gcgagaaggt ccggctgttc gccggcgtct ttgcgatttt cggccatggc aatgtcgccc
     2161 ggctgggcga agccttgcag gccgcgcaag cggcgctgcc gacctaccgc gcgcataacg
     2221 agcaggcgat ggggctttcc gccgtcgcct acgccaaggc gatgcgccgg cgccgggtga
     2281 tggcctgcac cagctcgatc ggccctggcg cgaccaatat ggtcaccgcc gcggccgtcg
     2341 cgcacgtcaa ccgcctgccg gtcctgtttc tgcccggcga cgttttcgcc aatcgccggc
     2401 ccgatccggt gctgcagcag gtcgaggatt tcggcgacgg cacggtcagc gccaatgatt
     2461 gcttcaagcc cgtctcgcgc tatttcgacc gcatcacgcg gccagagcag atcatcccgg
     2521 cgctaacgcg cgccgtcgcg gttctgaccg atccggcgga atgcgggccg gtcacgctcg
     2581 ccttctgcca ggacgtgcag acggaagcgt acgactatcc cgagacgttc ttcgacgagc
     2641 ggatgcattg gcggcgccgg cagggcccgg acccgcacga attgcgcgaa gccgccgcgc
     2701 tgctgcgcgg cgccaggaag ccgctggtcg tcgccggcgg cggcgtcctc tattccggcg
     2761 cggaaaagga gttgctcgcc ttctgcgaaa agcgcggcgt cccggtcgcc gagacgcaag
     2821 ccggcaaatc ggccgtgccg gccgatcatc ctctggcgct cggcgcgatc ggcgtgaccg
     2881 ggaccagcgc cgctaacgca tatgcggaag aggccgacgc gatcttggcg gtcggcgcgc
     2941 ggcttgccga cttcgtcacc ggctcttggg ctctgttcaa gaacccggag cgacggttcg
     3001 tcggctt
//
LOCUS       DATGJW010000352         2587 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_793435, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000352 DATGJW010000000
VERSION     DATGJW010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2587)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2587)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2587
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_793435"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(311..1732)
                     /locus_tag="VKU03_10735"
     CDS             complement(311..1732)
                     /locus_tag="VKU03_10735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011425915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PLP-dependent aminotransferase family protein"
                     /protein_id="HLJ71785.1"
                     /translation="MLHAPLADGRKLTRAEAVVEAVRARIDGRKLAPGARLPSVRGLA
                     QTMGVSKSTVVEAYDRLLADGAIVARRGSGFFVAGPTRPLSLAAIGPQLDRAIDPLWV
                     MRQSLQSGADMLKPGSGWLPETWMPDAAIQRGLRQMAREALETRTQYDAPLGFAPLRQ
                     QLALRMAERGVSAAPDQIILTNSASHALDLVCRFLIEPGDAVLVDDPCYFNFHALLRA
                     HRARIVGVPMTPDGPDVEAFGQILAAEKPRFYLAIAGLHNPTGATLGPVVAHKLLKLA
                     EKHDTLVVEDEVFADLEPSPATRMASFDGFERVIQVGSLTKTVSAAFRCGYIAAKPEW
                     IEELIDLKLATGGSSGRLSAVLLHRNLSEGGYRRHLEQLRTKLSDAMGLTLRRLEAAG
                     LTPWLEPRGGMFVWARLPGGLDAADIARIGLRRGVVFAPGNVFSLAPGPNPYLRFNVA
                     LCGPPRVFSVLEEAMNEAAHGRP"
     gene            1836..2171
                     /locus_tag="VKU03_10740"
     CDS             1836..2171
                     /locus_tag="VKU03_10740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008838623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1127 domain-containing protein"
                     /protein_id="HLJ71786.1"
                     /translation="MFHFREVVEPVAAPRRSLLWRVAAWPLRVLAARRAMAALGAMSD
                     RELADVGLYRQDLRDATALRLGEDPTALLTARTAERRGARWAAAVENPLAPRSGERVA
                     AKPPGEGLR"
BASE COUNT          431 a          840 c          912 g          404 t
ORIGIN      
        1 agcagcgcgc cgccggccgc attctgctgc tgcggcgtct cgcagccggc cagggcgatc
       61 gcgctggcgg cgaccgtcgt gagaagaacc aatgctttca tgcagaaaac tccgagattg
      121 cccttaaggc ccgcctgacc cggcggctct tagcacgggc ctagcccgcg ccttccttgg
      181 gagcgcagga cttaacgaat cggcgaaaaa tcgccccctc gccaagcaaa gcctcgcacg
      241 ccaattatga attgagggtg aaccttgggc gaaatgaggg cgaagccgcg acacgaatct
      301 ttaacaaggg ttaaggtcgg ccgtgcgcgg cctcgttcat cgcctcctcc aggacggaaa
      361 agacccgcgg cggcccgcat agcgcgacat tgaaacgtaa atagggattc gggcctggcg
      421 ccaggctgaa gacgttgccc ggcgcgaaaa ccacgcctcg cctcaggccg atgcgggcga
      481 tgtcggcggc gtcgagcccg ccgggcaggc gagcccagac gaacatgccg ccgcgcggct
      541 cgagccaagg cgtcaggccg gccgcctcca gccgccgcaa ggtcaagccc atcgcgtcgg
      601 acagtttcgt ccgcaattgc tcgagatggc gcctgtagcc gccctcgctg agatttcgat
      661 ggagcagcac ggcggagagg cggcccgacg agccgccggt cgcaagcttc aagtcgatca
      721 gctcctcgat ccattccggc ttggcggcga tataaccgca gcggaaagcg gccgagaccg
      781 tcttggtcag gctgccgacc tggatgaccc gctcgaaccc gtcgaacgag gccatgcgcg
      841 tcgctggcga cggttcgaga tcggcgaaga cttcgtcctc gacgaccagc gtatcgtgct
      901 tctcggccag cttgagcagc ttgtgggcga cgaccgggcc gagcgtcgcg ccggtcggat
      961 tgtgcaggcc ggcgatcgcc agatagaagc gaggcttttc cgccgccagg atctggccga
     1021 acgcttcgac atccggcccg tccggcgtca tcgggacgcc gacgatgcgg gcgcgatgcg
     1081 cgcggagcag cgcgtgaaaa ttgaaatagc aggggtcgtc gaccagcacc gcgtcgcccg
     1141 gctcgatcag gaagcggcag acgagatcga gcgcgtgcga cgccgaattg gtcaggatga
     1201 tctggtcggg cgcggcggag acgccgcgct cggccatgcg cagcgccaat tgctggcgca
     1261 gcggagcgaa gccgagcggc gcgtcgtatt gcgtgcgggt ctccagcgcc tcgcgcgcca
     1321 tctggcgcaa gccgcgctgg atcgcggcgt ccggcatcca ggtttcgggc agccagcccg
     1381 agccgggctt cagcatgtcc gcgccggact gcaaggattg gcgcatcacc cagagcggat
     1441 cgatggcgcg gtcgagctgc gggccgatcg ccgcgagcga cagcggccgg gtcgggccgg
     1501 cgacgaagaa gccggagccg cgccgcgcca cgatcgcgcc gtcggcgagc agccggtcat
     1561 aggcttcgac cacggtcgac ttggagacgc ccattgtctg ggcgaggccg cgcacggaag
     1621 gaaggcgcgc cccgggcgcc agcttgcggc cgtcgatgcg agcgcgaacc gcttcgacca
     1681 ccgcctcggc gcgggtcagc ttgcggccgt cggcgagcgg ggcgtgcaac atctgtattg
     1741 gcctctgtgg caggacagtt ttcaataact gtattggatt gtctctgccc tgtcgaggct
     1801 ggacctgcga tgctgcgcat cgaaaggcaa gcgccatgtt ccatttcagg gaagtcgtcg
     1861 aacccgtcgc cgcgccgagg cggtccctcc tgtggcgcgt cgcggcctgg ccgctgcgcg
     1921 tccttgcggc gcgccgggcg atggccgcgc tcggcgcgat gagcgatcgc gagctcgccg
     1981 atgtcggcct ctaccggcag gacttgcgcg acgccacggc gctgcggctg ggcgaggatc
     2041 cgacggcgct tctgaccgcg cggacggctg agaggcgagg ggcgcgctgg gcggcggccg
     2101 tggaaaatcc cctcgccccg cgcagcgggg agagggtggc cgcgaagccg ccgggtgagg
     2161 gactgcgctg agcttgggga ttagcggtcc gaaaggtacg tacctttcga gtccggcgca
     2221 gcctctcatc cgggcctgcg cgccgccttc tcccggcctt gcgcagggct gtccggggaa
     2281 agtctgaggt tatggcgcgt cttggacgta atctttcagc gccgcggcgc catcgacgcg
     2341 gggcggggtc aatcctcgcg cagcgcagcg aagcgaccgg cgaaagccgg ctcgggattg
     2401 acgccgcccc gcggcgatgg cagcgcggtg taggggattt gcgtcaagag tttgtgagca
     2461 gccccgaggt ccgcatgttt attttctgag tctcggcgcc ggaaactcaa cgaggaaatc
     2521 tcgaaatcgt gttatttcac gagacttcgt cgacgacgat gcgactcgcc ttttactttc
     2581 cccggcc
//
LOCUS       DATGJW010000353         2498 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_380285, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000353 DATGJW010000000
VERSION     DATGJW010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2498)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2498)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2498
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_380285"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..102
                     /locus_tag="VKU03_10745"
     CDS             <1..102
                     /locus_tag="VKU03_10745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011470698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate epimerase"
                     /protein_id="HLJ71787.1"
                     /translation="IEWRRSDDHVLMTGPVELEFETVLAPDLQSAAS"
     gene            99..1385
                     /gene="mtaB"
                     /locus_tag="VKU03_10750"
     CDS             99..1385
                     /gene="mtaB"
                     /locus_tag="VKU03_10750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181134.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (N(6)-L-threonylcarbamoyladenosine(37)-C(2))-
                     methylthiotransferase MtaB"
                     /protein_id="HLJ71788.1"
                     /translation="MSAIRIETFGCRLNLAESDAILREAERAGAEALSIVNSCAVTGE
                     ALRQARQAARRLKRQRPFAKVVVAGCGAEIDAAAFAAMPEVDAVIGNREKRDGAALAR
                     IAAGAAPRVMIGAAAPALPLIPAIAEARTRAFVEIQNGCDHRCTFCVIPLARGPSRSL
                     DPARIVADIQRLVASGVAEIVLTGVDITAYGLDAPGAPRLGGLVRHILAETPALKRLR
                     LSSIDSVEVDADLERAIADEGRLMPHLHLSLQSGDDLILKRMKRRHSRADAVRFCEEA
                     RRRRPNIAFSADIIVGFPTESEAMFENSLRLVGDCGLARAHVFPFSPRPGTPAARMPQ
                     IPADIVRERAARLRAASDAAWAKRLSSHVGRAAPVLMERGGVGRLEDFTPVRVEEIAQ
                     GALCEAWIEASDGRELKGRRRDCVGPTPVSALDSAA"
     gene            2180..>2498
                     /locus_tag="VKU03_10755"
     CDS             2180..>2498
                     /locus_tag="VKU03_10755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2794 domain-containing protein"
                     /protein_id="HLJ71789.1"
                     /translation="MSEVDPPQQRRAPLHESGALDAGVQDSQRGVVSPAKSSAIVSFS
                     RQELREIMDLYGRMVAAGEWRDYALDFTADEAVFSVFRRASETPLYRIKKDPKLARRQ
                     GAYS"
BASE COUNT          432 a          837 c          802 g          427 t
ORIGIN      
        1 atcgaatggc gcaggagcga cgaccatgtc ctgatgaccg gcccagtcga actggagttc
       61 gagacggttc tcgcgccgga tttgcaaagc gccgcgtcat gagcgcgatc cgcatcgaga
      121 cgttcggctg ccggctcaat ctcgccgagt cggacgcgat cctccgcgag gccgagcggg
      181 ccggcgcgga agcgctgagc atcgtcaatt cctgcgcggt gaccggcgaa gccttgcgcc
      241 aggcgcggca agcggcgcgg cggctgaagc gccagcggcc tttcgcgaaa gtcgtcgtcg
      301 ccggctgcgg cgccgagatc gacgccgcag ccttcgccgc gatgccggaa gtcgacgcgg
      361 tgatcggcaa tcgcgagaag cgcgacggcg cggcgctggc gcgcatcgcc gcgggcgccg
      421 cgccgcgcgt gatgatcggc gcggcggcgc cagcgctccc gctgatcccg gccatcgccg
      481 aggcccggac ccgcgccttc gtcgaaatcc agaacggctg cgaccatcgc tgcaccttct
      541 gcgtcattcc gctggcgcgc gggccctcgc gctcgctcga cccggcgcgg atcgtcgccg
      601 atatccagcg cctcgtcgcg agcggcgtcg ccgaaatcgt cctgaccggg gtcgacatca
      661 ccgcttacgg cctcgacgcg cccggcgcgc cgcgcctcgg cggcctggtc cgccacatcc
      721 tggccgagac gccggccttg aaacggctgc gcctctcctc gatcgattcg gtagaggtcg
      781 acgccgatct cgaacgggcg atcgcggacg aggggcgcct gatgccgcat ctccatttgt
      841 cgctgcaatc cggcgacgac ctgatcttga agcggatgaa gcgccgacac agccgcgccg
      901 acgccgtccg cttctgcgaa gaagcgcgcc gccggcggcc gaacatcgcc ttttccgccg
      961 atatcatcgt cggctttccg accgagagcg aggcgatgtt cgagaactcg ctgcgtctcg
     1021 tcggcgattg cggcctcgcc cgcgcccatg tctttccctt ctcgccgcgg ccggggacgc
     1081 ccgccgcccg catgccgcag attcccgccg acattgtccg cgagcgcgcc gcgcggctgc
     1141 gcgcggcctc ggacgccgcc tgggcgaaac gattgtcgag tcatgtcggc cgcgccgcgc
     1201 ccgtcctgat ggagcgcggc ggcgttgggc ggctggagga ttttacgccg gtgcgggtcg
     1261 aagaaatcgc gcaaggcgcg ctctgcgaag cctggatcga ggccagcgac gggcgcgaat
     1321 tgaaggggcg gcggcgagac tgtgtcggcc ccacgccagt ttctgcttta gactcagccg
     1381 cttagactca tctatgcgga aagcgaagac gcccaaagca catccgatgc ttcggcgaga
     1441 agcggagcta atgcatccgc tagcccgtcg aacccgtata gaggggcgga ccgcgacaag
     1501 ttcgaggacg ccgaactcgc tgcacaagac gacgcctgct tcacttttaa tgctcaactg
     1561 cagcgaggca tttcgcaggc gcgcccggat cgctgcgttc gagaacccgg acctaattta
     1621 caattgcacc gccgcgttgc gcgcctgcaa gagcaccggc ctcccaacaa tttttggccc
     1681 gggaattgtc gggaagaagt gaggaatgtc tatgccatcc gacgtttgga atgaagccga
     1741 tcaggttgca ttttcgaaag tgcaagacct ttatctacaa gatgcctatc acgactgcct
     1801 gatctcagcc tgcagcctgt tgacgcgcgc cagagataca agacaagcgg gactttatgc
     1861 gtcctggcgg aattcattcg gcaatgcgca gccgagttgg cgccgaaacg catggatagc
     1921 ggggcagagc ccacatgctc gttttgcgcg aaggcgccgc ccgccgtcag actaggcgca
     1981 ggtccctccg cattcatctg caacgaatgc gttgaccttt tctctgcggc tttggcaggc
     2041 gaccgaccta gttgaatgga ttccctgctt taatcccctc ttgctctgca cagagcttgg
     2101 cgcgatcatg aacggctttt gaacggcaag aggcggcccg tcgccgcgaa gccgtttgca
     2161 actaaaaggg aggcgcgatt tgagcgaagt cgatccgccg cagcagcggc gcgcgccgct
     2221 tcacgagagc ggcgcgcttg acgcaggcgt tcaggacagc caacgcggcg tcgtttctcc
     2281 ggccaaatct tccgcaattg tctcgttcag ccgccaggaa ttgcgggaaa tcatggacct
     2341 ttacggccgc atggtggcgg ccggcgaatg gcgcgactac gccctcgatt tcaccgccga
     2401 cgaagccgtt ttctcggtct tccggcgcgc ctccgaaacc ccgctttacc ggatcaagaa
     2461 ggacccgaag cttgcgcgcc gccagggcgc ctatagcg
//
LOCUS       DATGJW010000354         3038 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1416199, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000354 DATGJW010000000
VERSION     DATGJW010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3038)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3038)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3038
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1416199"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..436)
                     /locus_tag="VKU03_10760"
     CDS             complement(<1..436)
                     /locus_tag="VKU03_10760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ71790.1"
                     /translation="MDLTSAQKKTVLAAFLGWMLDAFDFFILVFVLKDIAKEFNVGVP
                     AVAYALFLTLAMRFIGAFIFGRIGDRVWRKPALMIDIVCYSVIGALAAFSPNLTIFLI
                     LRTLFGVAMGGEWGLGSSLAMESIPPKARGAVSGFLQCGYPTG"
     gene            complement(697..1131)
                     /locus_tag="VKU03_10765"
     CDS             complement(697..1131)
                     /locus_tag="VKU03_10765"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ71791.1"
                     /translation="MSSIEEIAARLRVVVRLLNRRVQTETGEGSPSRSEAAALAWLEE
                     RGPMTLGALAELEQVRQQSMSQSVEALQERGWATRSIDASDRRQVIIALTEAGRAALA
                     AGRGVRQAWIVDAMTRKLNAQERRLLAQSLDLLERIARAERR"
     gene            1224..2447
                     /locus_tag="VKU03_10770"
     CDS             1224..2447
                     /locus_tag="VKU03_10770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019857596.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="multidrug efflux MFS transporter"
                     /protein_id="HLJ71792.1"
                     /translation="MSATLTEADFNAGESVHWRRNLAVCLAGSFTTLIAMSLLLPFLP
                     LYIEELGVKDHAAIAQWSGAVYGVTFFSAGLVAPLWGRLADRYGRKLMLIRASFGMAI
                     VMSLIGMAHDVWQLFWLRLLVGFLGGYSSGSMTLVATQTPKAQAGWALGALTTGIMAG
                     NLVGPLIGGFLPPLIGIRATFWLAGGVIFIAFLATTFLIKEAPRPAASRGKSRAGWSA
                     LPDKRPIAAMLATGLLLMLANMSIEPIITLYVQQLAVDPDKVTTFAGLAMSAAALGSI
                     LSASRLGRLADRVGHWAVIAGGLAVCALLLIPQAFVTAAWQLVILRFFMGIALGGLLP
                     CIAAIIRHNAPDSVAGTMLGYSTSSQYAGQVVGPLAGGFVGGHFGMRSVFLATCLMMA
                     AGSAFIWAARPRKPR"
     gene            2525..2686
                     /locus_tag="VKU03_10775"
     CDS             2525..2686
                     /locus_tag="VKU03_10775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834825.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter permease"
                     /protein_id="HLJ71793.1"
                     /translation="MAEEENAGPKTYPADKARQGDIILRTPARRWIFIGGMLAALILV
                     LIAQYLYLK"
     gene            complement(2789..>3038)
                     /locus_tag="VKU03_10780"
     CDS             complement(2789..>3038)
                     /locus_tag="VKU03_10780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168778.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="putative PEP-binding protein"
                     /protein_id="HLJ71794.1"
                     /translation="DPFVTIDQDGVGELIALATERGRKTRPSLKLGICGEHGGDPASI
                     AFCEHVGLDYVSCSPFRVPIARLAAAQAALGRKAASTA"
BASE COUNT          493 a         1009 c          975 g          561 t
ORIGIN      
        1 agccggtcgg atagccacat tgcaggaagc ctgacacggc gccgcgcgcc ttgggcggaa
       61 tcgattccat ggcgagcgag gagccgagcc cccattcgcc gcccatggcg acgccgaaca
      121 gcgtgcgcaa gatgaggaat atcgtcagat tgggcgagaa ggcggcgagc gcgccgatga
      181 ccgaatagca gacgatgtcg atcatcagcg ccggcttgcg ccagacccgg tcgccgatgc
      241 ggccgaagat gaacgcgccg atgaagcgca tcgccagcgt gaggaacaag gcgtaagcga
      301 ccgccggcac gccgacattg aactccttgg cgatgtcctt gagcacgaaa accaggatga
      361 agaaatcgaa ggcgtcgagc atccagccga gaaaagcggc cagaaccgtc ttcttttgag
      421 cgctcgtcag atccatagcc gctccccctc tttaacccgc ttgcgccaat tggccatgcg
      481 cctcaatgag gcgcaagcga aatgttgagg ggctaatccc ttctccccgc ttcgcgggga
      541 gaaggtggcg cgaagcgccg gatgaggggc tgcgccggac tagaaggtac gctctgtacc
      601 tgtagctagg agaataactt tccggctccg cgccgcctct catccggccg agcttcgctc
      661 ggccaccttc tccccgcttt gcgaggagaa ggggattcac cgcctttcgg ccctggcgat
      721 gcgctcgagc aggtcgagcg attgggcaag gagccggcgc tcctgggcgt tcagctttcg
      781 cgtcatcgcg tcgacgatcc aggcttgccg cacgccgcgg ccggccgcga gagccgcccg
      841 gcccgcctcg gtcaaagcga tgatcacctg ccggcggtcg ctggcgtcga tcgaccgcgt
      901 cgcccagccg cgctcctgca aagcctcgac gctctggctc atcgattgct ggcggacctg
      961 ctcgagctca gccagagcgc cgagcgtcat cggcccgcgc tcctcgagcc aggcgagcgc
     1021 cgccgcctcc gagcggctcg gcgagccttc gccggtctcg gtctgcacgc ggcgattgag
     1081 cagccgcacc acgacgcgca gccgcgcggc gatttcctcg atcgagctca tttatacagt
     1141 ttacctgttt atcaggcgta ctgtatatta accggcggcg catccttttc caagcccgcg
     1201 ttaaccattt tccggaaggc gagatgagcg cgacgttgac cgaagcggat ttcaacgccg
     1261 gggaatccgt tcattggcgg cgcaatctcg ccgtgtgcct cgccggctcg ttcacgacgc
     1321 tgatcgcgat gtcgctgctt ttgccgttcc tgccgctcta tatcgaggag ctcggcgtca
     1381 aggatcatgc ggcgatcgcg caatggtctg gcgcggtcta tggcgtgacc tttttctcgg
     1441 ccggcctggt cgcgccgctc tggggccgcc tcgccgaccg ctacggccgc aaattgatgc
     1501 tgatccgcgc cagcttcggc atggcgatcg tgatgtcgct gatcggcatg gcgcatgacg
     1561 tgtggcaatt gttctggctg cgcctgctgg tcggctttct cggcggctat tcgtccggct
     1621 cgatgacgct tgtcgcgacg cagacgccga aagcccaggc cggttgggcg cttggcgcgc
     1681 tgacgaccgg catcatggcc ggcaacctcg tcggtccctt gatcggcggc tttctgccgc
     1741 cgctgatcgg cattcgcgcc acgttctggc tcgccggcgg cgtcattttc atcgcctttc
     1801 tcgccacgac ctttctgatc aaggaggcgc cgcggccagc ggcaagccgc ggcaaatcgc
     1861 gcgccggctg gtccgccttg ccggacaagc ggccgatcgc cgcgatgctg gccaccggcc
     1921 tcttgctgat gctcgccaat atgtcgatcg agccgatcat cacgctttac gtccagcaac
     1981 tggcggtcga tccggacaag gtgacgacgt tcgccggcct cgcaatgtcc gcggcggctt
     2041 taggcagcat cctatccgcg tcgcgcctcg gcaggctcgc cgaccgcgtc ggccattggg
     2101 cggtgatcgc cggcggcctc gccgtctgcg cgctgctgct cattccacaa gccttcgtaa
     2161 cggccgcctg gcagctcgtg atcctgcgct tctttatggg gatagcgctg ggcggcctgt
     2221 tgccctgcat cgccgcgatc atccgccaca acgcgccgga ttcagtggcg gggacgatgc
     2281 tcggctattc gacatcctcg caatatgccg gacaagtcgt cggtccgctg gccggcggct
     2341 ttgtcggcgg ccatttcggc atgcgctcgg tgttcctcgc gacctgcctg atgatggccg
     2401 cgggctcggc tttcatatgg gcggcgcgac cgcgcaagcc gcgctagcct cgaccgccgg
     2461 aacaccggca gatatcattc gttgtgcttg caaaagcgcg ctcgcgagcc gtcaggagag
     2521 cgccatggcc gaagaagaaa acgccggacc gaagacctat cccgccgaca aggcccggca
     2581 aggcgacatc atcctgcgca cgccggcgag gcgctggatt ttcatcggcg gcatgctggc
     2641 ggcgctgatc ctcgttctca tcgcgcagta tctgtacctg aagtgaaggc gctccgtcct
     2701 ggcgagcgca gcgaagcatc cattcgtaga gcgtcgtcct ataaatggat tgcttcgtcg
     2761 cttcgctcct cgcaatgacg ggtaggcgct acgccgtcga cgccgctttc ctgcccagcg
     2821 ccgcttgcgc ggcggcgagg cgggcgatgg ggacgcggaa gggcgagcag gagacgtagt
     2881 cgagcccgac atgctcgcaa aaggcgatgc tggccggatc gccgccatgc tcgccgcaaa
     2941 tgccgagctt gagcgaaggc ctggtcttcc gcccgcgctc ggtggcgagc gcgatcaatt
     3001 cgccgacgcc gtcctggtcg atcgtgacga agggatcg
//
LOCUS       DATGJW010000355        12699 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_67533, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000355 DATGJW010000000
VERSION     DATGJW010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12699)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12699)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12699
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67533"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..251
                     /locus_tag="VKU03_10785"
     CDS             <1..251
                     /locus_tag="VKU03_10785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HLJ71795.1"
                     /translation="GSFYPPDVIKAYEAGNHNDSTVGDVYFRAADHQLVRPVIIVRGK
                     KPGDMKNKEDYWEVLEVVPGEGLMQKPDAFGCNLGSYT"
     gene            352..1227
                     /locus_tag="VKU03_10790"
     CDS             352..1227
                     /locus_tag="VKU03_10790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ71796.1"
                     /translation="MITWANFVSQLFNGLALGALLALIASGLTIIYGTLGVLNLAHGA
                     MFMLGGYAGYVAYQATGSFVLAIVAGSLFLVIVGVVMERLIIRHFYGRPLEDQLLVTF
                     GLGICYVEAVRFFFGSLSKTVPAPAWASGITSLGFMFYPTYRLVVVGVVAAALLVLYV
                     ALYRSRLGLIVRAGIEDSGMVGMLGINVYRVFMIVFGIGAMAAGFAGIINAPVVSLSP
                     DVGDAILVQTFVVVVIGGVGSFFGAILGGLISGEILSLTSMVNPAYSGVMLFAAMTLV
                     LLLRPQGLLGARSRE"
     gene            1241..3064
                     /locus_tag="VKU03_10795"
     CDS             1241..3064
                     /locus_tag="VKU03_10795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     ATP-binding protein/permease"
                     /protein_id="HLJ71797.1"
                     /translation="MLTRRPFLVEALTAIGLIAAPFILPHFGFAPNTVNQILVWGLFG
                     IGFDILFGFAGLLSFGQAAFYGTGGFVAAYLLTVAGLSNVILGLAIGTVAAAAVGYVV
                     GLIALRRTGIYFAMITVAIAETFFFLENNPLSAWTGGENGLPGVPTPKFDLFGFHYEV
                     GVGWSMYGFLAVCYFLGIVVALRIVRSPVGAIFSAIRDNPLRAAAVGHNVHGYKLMAF
                     VIAAAYAGLAGGLLGVLQAFMPPEAFTFDTSGQLVMQTAIGGGGALFGPLLGAAVWLS
                     LRDFLQGALGLGAAWKLVLGIVFVLLVCFLRHGLIGGLRDLVRLFVRRAEPPAAEPAA
                     QAAAPALASLGPARRRDETPFSGPALEARGVTKSFGGLVANRDINFSVQPGELRGVIG
                     PNGAGKSTFFKMLTCEMAPSAGRIHFHGRDIAGMDIASVCQLGLTKSYQVNQLFTRLT
                     LRQNLVIAALAERRGKFSFDLLRRLDRVPGLATQVESTLAMVGLTHRADTPVAELAYG
                     EKRRLEIGLALATSPTVLLLDEPLAGMSPQERRETVQLLKNIRRGRTLIVIEHDMDAI
                     FNLAERITVLHEGRVLAEGTPEEIQGNRFVQDAYLGGVAAA"
     gene            3061..3759
                     /locus_tag="VKU03_10800"
     CDS             3061..3759
                     /locus_tag="VKU03_10800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018452888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71798.1"
                     /translation="MSLLEVENLNSYYGDSHILFDVALHVEQREVVALLGRNGAGKST
                     TLKSLMGAVKPRAGTVRLDGVSLAGWPAHAIAQAGMQLVPEERRIFGSLNVEENLVLA
                     GLSATNRWPLGRIYEMFPRLKERRSSRGTDLSGGEQQMLAIARALMRDPKIILLDEPF
                     EGLAPIIVRDLMKACRQLADSGQTIVVVEQNLAAALSLAHRAYIINNGHIVHEASTQE
                     IKSAPDLVQRWLGV"
     gene            complement(3922..4863)
                     /locus_tag="VKU03_10805"
     CDS             complement(3922..4863)
                     /locus_tag="VKU03_10805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014129415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ71799.1"
                     /translation="MAHSIAIIGIGKITQDQHLPVIAKNPNFKLAALVSQRGLRQAGV
                     PTFRTPAELYAALPDVETVAICTPPAVRHAIAREALDAGKHVLLEKPPTPTTAELDDL
                     VAHAARRSRVLFATWHSQYNSAVDEARRRLAGRKLHSLHIEWKEDVKRWHPGQEWIWD
                     AGNFGVFDPGINALSILTKIAPAPIFVKSAALSYPANRDTPIAASLVFSSLAAAGPAS
                     TFTAEFDWRQEGEQSWNIDIETAEGEKLRLSHGGSKLFAGGALVLEKPMAEYEGIYAH
                     FADLLAAKKSAVDRAPLQLVADAFLVGKRLAVDAFHW"
     gene            complement(5141..6160)
                     /locus_tag="VKU03_10810"
     CDS             complement(5141..6160)
                     /locus_tag="VKU03_10810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar-binding protein"
                     /protein_id="HLJ71800.1"
                     /translation="MLKNLFTAAVAAGALLVLASGANAAEKKYTFALVPKNTNNPFFD
                     QALAGCKKAEKELAGKIECLYIGPGEHGGGDEEAQIVADLITKKVDGIGVSPANAAAM
                     AAALQGAKAANIPVLTWDSDLLDKDKGLRAAYIGTHNYDIGVNLAKIVMQIKPKGGTI
                     CIQSGGAAAANHNERMQGIRDTLAGKKSAEAPGEKLTGQNGWTEVAGCPLYTNDDFAV
                     ANQQMEDILNKYPDLDAFVPTGGFPQFLPDAYTKTAEKVKDKIASGKLALVVADTLPV
                     QIELMKKGLSAGQVGQRPFEMGYKVMYALLDMKEGKPAPHDPTYTGLDVCTPKTADTC
                     IGGGK"
     gene            complement(6213..7316)
                     /locus_tag="VKU03_10815"
     CDS             complement(6213..7316)
                     /locus_tag="VKU03_10815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007818346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71801.1"
                     /translation="MSLETQARPDKASTLSIAEPILDTTEKTFVERLLGNQALWVTLT
                     LIILCIVGSVFQENYASEDNFYNTTRNFAFIGVMALGMTPVIIIGGIDLSVGSVMGIA
                     AIVCGLVLLKQPIDWMPAWWNSAVWAHTWWMAVILSLAAGALVGLINGVLIAYVGLPP
                     FVVTLGMLSAARSVAVILSGNRMLYSFVPEQAKVLKSIGGADNFGISLPYQLRLSNPF
                     LILVVLTFVLGIVLKMAGWGRHIFAIGGNEQAANLTGVPVRRIKVQAYVLCSMSAALA
                     GMLSIGLYGSANNSLGQGYELLAIAAAVIGGANLMGGEGTAYGAFIGAALIFVIRNLL
                     LFLGFDSNWQGIFVGSFIVLAVVLEKVRGKRRE"
     gene            complement(7306..8046)
                     /locus_tag="VKU03_10820"
     CDS             complement(7306..8046)
                     /locus_tag="VKU03_10820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183663.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ71802.1"
                     /translation="MPVLELSNITKHFGAIHALNGVDMNLEPGEALGLMGDNGAGKST
                     LVKIIAGNFPPTGGEIRIDGEPVQFAKPVEAREKGIEIVYQDLALCNNLTAAANVFLG
                     REIKTGIGPIRWLNHAAMFKQAGELFAELKSETRPRHLVRQMSGGQRQAVAIARTRLS
                     NPKIVLMDEPTAAISVRQVAEVLDLIKRLRDHGIGVVLISHRMPDVFAVCDRVVVMRR
                     GRKVADKKIKDSSPEEVTGLITGAIHVA"
     gene            8316..11135
                     /locus_tag="VKU03_10825"
     CDS             8316..11135
                     /locus_tag="VKU03_10825"
                     /EC_number="2.7.7.59"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008773 - [protein-PII]
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="[protein-PII] uridylyltransferase"
                     /protein_id="HLJ71803.1"
                     /translation="MNSSLAVKARAARAGRAAAVPRRSDRARLIADIAALERPANDDG
                     AAFRQAGVKLAAAALQEGRDEARRLLEAGGKGMACAERLSELQDDVIAAIHALALREI
                     RGDQPGEGDQPMAIAAVGGYGRGALAPGSDIDLLFLLPTKPTPGSEKLVETMLYMLWD
                     LRQKVGHSTRSIGECMRQAKADMTVRTSLLEARFILGDEALFEEMRKRFDAEIVAKSA
                     EDFVAAKLAERDERLRRAGGSRYLVEPNVKDGKGGIRDLNTLFWIGKYVYRVRHARDL
                     VEAGLFDAREYALFRRCEEFLWAVRCHLHFLAGRAEDRLGFEFQPAIAERLGYMSRFG
                     LTEVERFMKHYFLIAKDVGDLTAIVCAALEERQAKPRAALDRFIGRLRRRARIVAGAA
                     DFIVDHGRISVVNARVFERDPINLIRLYWAADRSGLAVHPDATRLVTKSLNRIDAKLR
                     NDPEANRLFLEVLTSRRAPETVLRLMNEAGVLGRFIPDFGRIVGMMQFSMYHHYTVDE
                     HLLRTIGVLAGLRSGALVEEHPLASEFLPGIANPSLLYVAAFLHDIAKGGPEDHSIAG
                     AAVARRLCPRLGLSPGETETVAWLVEQHLAMSMTAQSRDLSDPRTIEAFAAIVQTLER
                     LRLLLVLTICDIRAVGPGVWNGWKGQLLRTLFWETEVVLSGGHSAADRKARVAESQEA
                     LRQALPGWSDPEFDAYAARLYPAYWLRVDLPHRVAHAKLLHAMAKETRSLSTEFSTDA
                     FRGATELTVVAPDHPRLLAVIAGACAASGGNIVDAQIFTTTDGLALDTISLSRAFDRD
                     EDELRRAGRIAKGVEDALRGKIRLTDIIAAKRKPDPRAGAFTVAPEVTISNSLSNRFT
                     VIEISGLDRPGLLYEVTSALSNLNLNIGSAHIVTFGEKAVDTFYVTDLTGSKIASPAR
                     QAAIKRHMLAIFDGPDEKKPAAGGR"
     gene            11216..11803
                     /gene="grpE"
                     /locus_tag="VKU03_10830"
     CDS             11216..11803
                     /gene="grpE"
                     /locus_tag="VKU03_10830"
                     /inference="COORDINATES: protein motif:HMM:NF010739.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000774 - adenyl-nucleotide exchange
                     factor activity [Evidence IEA];
                     GO_function: GO:0042803 - protein homodimerization
                     activity [Evidence IEA];
                     GO_function: GO:0051087 - protein-folding chaperone
                     binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide exchange factor GrpE"
                     /protein_id="HLJ71804.1"
                     /translation="MKKDKDEAATAPLSGDGGANAAEQAAPDPAAVIETLNAENASLK
                     DRLLRAMAEMENLRRRTEREIADAKTYGVASFARDMLTVADNLGRALDSLPEEARAGG
                     AEGALKGLIEGVELTMRDLTAALARHGVKKLEPLGEKFDPNFHEALYEAPNEELPAGT
                     VAQVVQSGWRIGDRVLRPARVGVSKGGPKVAAKAE"
     gene            complement(11896..12525)
                     /locus_tag="VKU03_10835"
     CDS             complement(11896..12525)
                     /locus_tag="VKU03_10835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoglutarate-dependent dioxygenase AlkB"
                     /protein_id="HLJ71805.1"
                     /translation="MTAPARGLEIIPGLVHYPAYLDRPAQEALRDALGEALRAAPLFT
                     PRMPRTGKPFSVRMTNCGPLGWVSDEAGYRYQPAHPATGAPWPPIPAIALAAWADLAT
                     GAAPPEACLVNVYDASARMGLHQDRDEEDFSAPVLSLSLGDSCLFRVGGSKRGDRTRS
                     FELNSGDALILGGPARLAFHGVDRIRGGSSTLLAGGGRINLTLRRVTRG"
BASE COUNT         2042 a         4273 c         4095 g         2289 t
ORIGIN      
        1 ccggcagctt ctatccgccg gacgtgatca aggcctacga ggccggcaat cacaacgact
       61 ccaccgtcgg cgacgtttat ttccgggccg ccgatcatca gctcgtgcgg ccggtgatca
      121 tcgtccgcgg caagaagccg ggcgacatga agaacaagga ggattactgg gaggtcctcg
      181 aagtcgtccc gggcgaggga ttgatgcaga agccggacgc gttcggttgc aatctcggct
      241 cctatacctg acaataggtc gcgccggcgt cgcgggccgt cctccgggcc cgcggcgccg
      301 cgatgtgcgg ccgcttggcg ccgcgtccgc ttcgttggct gactcgccgc catgatcacc
      361 tgggccaatt tcgtctcgca attgttcaac ggcttggcgc tcggcgcctt gctggctttg
      421 atcgcctccg gcctgaccat catttatggg acgctcggcg tgctgaacct cgcgcatggc
      481 gcgatgttca tgctcggcgg ctatgccggt tatgtcgcct accaggcgac gggctccttc
      541 gtcctcgcga tcgtcgccgg ctcgctattt cttgtgatcg tcggcgtcgt gatggagcgc
      601 ctgatcatcc gccatttcta cggccggccg ctcgaagacc agcttctcgt cacgttcggc
      661 ctcggcatct gctatgttga ggccgtacgg ttctttttcg gcagcctgtc caagaccgtc
      721 cccgcgccgg cctgggcctc gggcatcacc tcgctcggct tcatgttcta tccgacctac
      781 cgcttggtcg tggtcggcgt ggtcgcggcc gctttgctcg tcctctatgt cgccctttac
      841 cggtcgcggc tcggcctgat cgtgcgggcc ggaatcgagg attccggcat ggtcggcatg
      901 ctcggcatca atgtctatcg cgtcttcatg atcgtcttcg gcatcggcgc catggccgcc
      961 ggcttcgccg gcatcatcaa cgcgccggtc gtctcgctga gccccgatgt cggcgatgcg
     1021 atcctggtgc aaaccttcgt cgtcgtcgtc atcggcggcg tcggctcgtt cttcggcgcg
     1081 atcctcggcg ggctgatctc cggggaaatc ctcagcctga cttcgatggt caatccggcc
     1141 tattccggcg tcatgctgtt tgcggcgatg acgctggtcc tgctgttgcg gccgcaaggc
     1201 ctgctcggcg cgcgcagccg cgaatgagac cgcccgtccc atgctgaccc gccgtccctt
     1261 tctggtcgaa gcgctgaccg cgatcggtct gatcgccgcg ccatttatcc tgccgcattt
     1321 cggctttgcg ccgaacaccg tgaaccagat cctggtctgg ggcctgttcg gcatcggctt
     1381 cgacatcctg ttcggctttg ccggcctgct ctctttcggc caggcggcgt tctacggaac
     1441 cggcggcttc gtcgcggcct atctgctcac cgtcgccggc ctctccaacg tcatattggg
     1501 cctggcgatc ggaacggttg cggcggccgc ggtcggctat gtggtcgggc tgatcgcgct
     1561 ccggcgcacc ggcatctatt tcgcgatgat cacggtcgcc atcgccgaga cgttcttctt
     1621 cctcgagaac aatccgctct cggcctggac cggcggcgag aacggcctgc ccggcgtgcc
     1681 gacgccgaaa ttcgacctgt tcggcttcca ttacgaggtc ggcgtcggct ggtcgatgta
     1741 cggcttcctg gcggtgtgct atttcctcgg catcgtcgtc gccctgcgca tcgtccgctc
     1801 gccggtcggc gcgatcttca gcgccatccg cgacaatccg ctgcgcgccg ccgcggtcgg
     1861 gcacaacgtc cacggctata agctgatggc cttcgtcatc gccgccgcct atgccggcct
     1921 cgccggcggc ctgctcggcg tgttgcaggc tttcatgccg ccggaggcgt tcaccttcga
     1981 tacgtccggc caattggtca tgcagacggc gatcggcggc ggcggcgctt tgttcggccc
     2041 gcttctcggc gccgcggtct ggctgtcctt gcgcgacttc ctgcaaggcg cgctcgggct
     2101 tggcgcggcg tggaagctgg tcctcggaat cgtcttcgtc ctgctggtct gcttcctccg
     2161 ccatggcctg atcggcggcc tgcgcgatct cgtcaggctc ttcgtgcggc gcgccgagcc
     2221 gcctgccgcc gagccggcgg cgcaggccgc agcgccggcc ttggcgagcc tcggccccgc
     2281 ccgccgccgc gacgagacgc cgttttccgg ccccgccctc gaagcgcgcg gcgtcaccaa
     2341 atcgttcggc ggcctggtcg ccaaccgcga catcaatttc tcggtgcagc cgggcgagct
     2401 gcgcggcgtc atcggtccga acggcgccgg caagagcacg ttcttcaaga tgctgacctg
     2461 cgagatggcg ccgagcgcgg gacggattca tttccacggc cgcgacatcg ccggcatgga
     2521 catcgcgtcg gtgtgccagc tcggcctgac caagagctat caggtcaacc agctcttcac
     2581 ccgcctgacg ctgcgccaga acctggtgat cgccgccctg gctgaaagac gcggcaaatt
     2641 ctccttcgat ctgctcaggc ggcttgaccg cgtgccgggc ctcgcgacgc aggtcgaaag
     2701 cacattggcg atggtcgggc tgacgcaccg cgccgatacg ccggtcgccg agctcgccta
     2761 tggcgagaag cggcggctcg aaatcggcct cgccctcgcc acgtcgccga ccgtcctgct
     2821 cctcgacgag ccgcttgccg gcatgagtcc gcaggagcgt cgcgagacgg tgcagctctt
     2881 gaagaacatc cgccgcggcc gcaccctgat cgtcatcgag cacgacatgg acgcgatctt
     2941 caacctcgcc gaacggatca ccgtgctgca tgaaggccgc gtcctggcgg aaggaacgcc
     3001 ggaagagatc cagggcaaca ggttcgtgca ggacgcctat ctcggcggag tcgccgccgc
     3061 atgagcctgc tcgaagtcga gaatctcaac agctattacg gcgattcgca catccttttc
     3121 gacgtcgctc ttcacgtcga gcagcgcgag gtcgtcgcgt tgctcggccg caacggcgcc
     3181 ggcaagagca cgacgctgaa gagcctgatg ggcgcggtca agccgcgcgc cggaacggtg
     3241 cggctcgacg gcgtctcgct cgccggctgg cccgctcacg ccatcgccca agccggcatg
     3301 cagctcgtgc cggaagagcg gcggattttc ggcagtttga acgtcgagga aaacctggtt
     3361 ctggccggcc tgtcggcgac caaccgctgg ccgctcggcc gcatctacga aatgtttccc
     3421 cggctgaagg agcggcgctc gagccgcggc accgatctct ccggcggcga gcagcagatg
     3481 ctggcgatcg ctcgcgcgct catgcgcgat ccgaagatta tcctgttgga cgagccgttc
     3541 gagggcctcg cgccaatcat cgtgcgcgat ctgatgaaag cctgccgcca actcgccgat
     3601 tcgggacaga cgattgtcgt tgtcgagcag aacctcgccg cggcgctatc gctcgcccac
     3661 cgcgcctata tcatcaataa cggccacatc gtccacgaag cctcgacgca ggagatcaag
     3721 agcgcgcccg acctggtgca gcgctggctc ggcgtgtgaa cgcttcctcc cccgtgaagc
     3781 gaagcggaac gggggagggg gaccggccaa aggccggggg agggggcctg cggcggttga
     3841 ggattatcct gagagaccgg cgccccctcc accgcgctga cgcgcgcccc tccaccgctt
     3901 tcgcggggga ggaaaagcgc gctaccagtg aaacgcgtcg accgccaggc gcttgccgac
     3961 caggaaggcg tcggcgacga gttgcagcgg cgcgcggtcg accgcgcttt tcttggcggc
     4021 gagcaagtcg gcgaaatgcg cgtagatgcc ttcgtactcc gccatcggct tttccagaac
     4081 cagggcgccg ccggcgaaaa gcttcgagcc gccatggctg aggcgcagtt tctcgccctc
     4141 cgccgtctcg atgtcgatgt tccagctctg ctcgccttcc tgccgccagt cgaactcggc
     4201 ggtgaacgtt gatgccgggc ctgccgccgc caggctcgaa aagacgagcg aggccgcgat
     4261 cggcgtgtcg cgattggcgg gataggagag cgcggcgctt ttgacgaaaa tcggcgccgg
     4321 cgcaatcttg gtcaggatcg acagcgcgtt gattccggga tcgaacacgc cgaaattgcc
     4381 ggcgtcccaa atccattcct ggccgggatg ccagcgttta acgtcctctt tccattcgat
     4441 atggagcgag tgcagcttgc gcccggcgag gcggcgccgc gcctcgtcga cggcggaatt
     4501 gtactgcgaa tgccaggtcg cgaagagcac gcggctgcgc cgcgccgcat gggcgacgag
     4561 gtcgtcgagc tcggccgtgg tcggcgtcgg cggcttttcg agcaggacat gcttgccggc
     4621 gtcgagcgct tcgcgggcga tcgcatggcg cacggccggc ggcgtgcaga tcgccaccgt
     4681 ctcgacgtcc ggcaaagccg catagagctc ggccggcgtg cggaaggtcg gaacgcccgc
     4741 ttgccgcagc ccgcgctggc tcaccagggc ggcaagcttg aagttggggt tcttggcgat
     4801 gaccggcaga tgctggtcct gcgtgatctt gccgatgccg atgatcgcga tcgaatgcgc
     4861 cacgtcgtgc tcctctgcct tccctttacg cagtggtcgc ggccgccccc actcgctgcc
     4921 ttcagcggct taccctcatc cggcgcttcg cgccaccttc tcccgcaagc gggagaaggg
     4981 attcgccggc agtcgacgat tgcgacgaag tccggcggtc ggcgccccct ctcccgcttg
     5041 cgggagaggg ttggggtgag ggcgagcctc ggtgcggacg tactttatga aaaaggcggg
     5101 gccgaagccc cgccttccaa cgcgttcgcc ggaacggcgc ttacttgccg ccgccgatgc
     5161 aggtgtcggc ggtctttggc gtgcagacgt cgaggcccgt ataggtcggg tcgtgcggcg
     5221 ccggcttgcc ttccttcatg tcgagcaggg cgtacatcac cttgtagccc atctcgaacg
     5281 gacgctggcc gacctggccg gccgacaggc ccttcttcat cagctcgatc tgcaccggca
     5341 gggtgtcggc gacgacgagc gcaagcttgc cggaggcgat cttgtccttc accttttccg
     5401 ccgtcttcgt ataggcgtcc ggcaggaatt gcgggaagcc gccggtcggc acgaaggcgt
     5461 cgaggtccgg atacttgttg aggatgtcct ccatctgctg attggcgacc gcgaagtcgt
     5521 cattcgtata gagcgggcag ccggcgactt ccgtccagcc gttctggccg gtcagcttct
     5581 cgccaggcgc ttccgcgctc ttcttgccag cgagcgtgtc gcggatgccc tgcatgcgct
     5641 cgttgtgatt ggccgccgcg gcgccgcccg actggatgca gatcgtgccg ccctttggct
     5701 tgatctgcat gacgatcttg gcgaggttga cgccgatgtc atagttgtgc gtgccgatat
     5761 aggcggcgcg caggccctta tccttgtcga gcaaatccga gtcccaggtc aggaccggaa
     5821 tgttggcggc cttggcgcct tgcaacgctg cggccatcgc cgccgcattg gccggcgaga
     5881 cgccgatgcc gtcgaccttc ttggtgatca ggtcggcgac gatctgcgcc tcttcatcgc
     5941 cgccgccatg ctcgccgggg ccgatataga ggcattcgat cttgccggcg agctccttct
     6001 cggccttctt gcagccggcg agcgcctgat cgaagaacgg attgttggtg ttcttcggca
     6061 ccagcgcgaa cgtatatttc ttctccgccg cgttggcgcc gctcgccaag acgagcaacg
     6121 cgccggcggc gacggccgcg gtgaatagat ttttcaacat gttccaccct ccactgttga
     6181 gctttctccc gatttcgcga cgcccagccg ggtcattcgc gtctcttgcc gcgcactttt
     6241 tcgagtacga cggcgagaac gatgaagctg ccgacaaaga ttccttgcca attcgaatcg
     6301 aaccccagaa acagcagcag attcctgatg acgaaaatca atgcggcgcc gataaaggcg
     6361 ccatacgcgg tcccttcgcc gcccatgaga ttggcgccgc cgatcaccgc ggcggcgatg
     6421 gcgagaagct cgtagccctg gccgaggctg ttattggccg agccgtagag cccgatgctg
     6481 agcatgcccg ccaaggcggc cgacatcgag cacaggacat aggcctgcac cttgatccgc
     6541 ctgaccggca cgccggtcag gttcgccgcc tgctcgttgc cgccgatcgc gaatatgtgc
     6601 cggccccatc ccgccatctt cagcacgatc ccgagaacga aggtcaggac gacgagaatg
     6661 agaaacggat tggagagccg aagctggtac ggcaggctga tcccgaaatt gtcggcgccc
     6721 cctatgcttt tgagaacctt ggcctgttca ggaacgaaac tataaagcat ccggttgccg
     6781 gaaaggatca cggcgaccga gcgggcggcc gacagcatgc cgagcgtgac gacaaagggc
     6841 ggcaggccga cataggcgat caaaacgcca ttgatcaatc cgaccagcgc tccggcggcc
     6901 aagctgagga tgacggccat ccaccacgta tgggcccata ctgcgctgtt ccaccaggcg
     6961 ggcatccaat cgatcggttg ttttaaaaga acgaggccgc agacgatcgc ggcgatgccc
     7021 atgaccgagc cgaccgagag gtcgatgccg ccgataatga tcaccggcgt catgccgagc
     7081 gccatgacgc cgataaaggc gaaattgcgc gtggtgttgt agaaattatc ttcgctggcg
     7141 tagttctcct ggaacacgct gccgacgatg cagagaatga tcagggtcag cgtgacccag
     7201 agggcttggt tgcccaagag cctctcgacg aatgtcttct cggtcgtatc gaggatcggc
     7261 tcggcaatcg acagcgtcga cgccttgtcg gggcgagctt gcgtttcaag cgacatggat
     7321 cgccccggtg atgagacccg tgacttcctc gggcgacgaa tccttgatct tcttgtcggc
     7381 gactttgcgg ccgcggcgca tcacgacgac gcggtcgcag accgcgaaaa cgtcgggcat
     7441 gcggtgactg atcagaacca cgccgatgcc gtgatcgcgc aggcgcttga tcagatcgag
     7501 cacttcggcg acctggcgca cgctgatcgc cgcggtcggc tcgtccatca gcacgatttt
     7561 gggattggag aggcgggtgc gggcgatcgc cacggcctgc cgctggccgc ccgacatctg
     7621 gcgcaccaga tggcgcggcc gcgtttccga tttcaattcg gcgaacaact cgccggcctg
     7681 cttgaacatg gcggcatgat tgagccagcg gatcgggccg attccggtct tgatctcgcg
     7741 gccgagaaag acgttggccg cagcggtcag attgttgcag agggcgaggt cttgatagac
     7801 gatctcgatg cccttctcgc gggcttcgac cggcttggcg aactggaccg gctcgccgtc
     7861 gattcggatt tcgccgccgg tcggcgggaa attgccggcg atgatcttga cgagcgtcga
     7921 tttgccggcg ccgttatcgc ccatcaaccc caaagcttcg cccggctcta gattcatatc
     7981 gacgccgttc aaggcgtgga tcgcgccgaa atgcttggta atattactca gttccaaaac
     8041 aggcaaagaa cggtcctccc aaccggcttt tgcgtcggga ggcgtcgtcc gccgcgcccc
     8101 cggtccgtgt tagaatgcta gcttccaata gttgcgggac ttcggcaaga gcaaaagggc
     8161 gtcatcttta cagtacgtcg actcgccggc cgcccctggg gaaccgagcc gcgccatgcc
     8221 gtccgcgtaa aagtctgact ttttcttgac gaaaaacccc cgcaagtcaa gtttgttcgg
     8281 cccgtcgtgg cgcggtccat gctaaggctc cggcgatgaa cagctctctt gccgtcaagg
     8341 cccgcgcggc gcgcgccggc cgcgccgccg ccgtcccgcg gcgcagcgac cgcgcgcggc
     8401 tgatcgccga catcgccgcg ctcgaacgcc cggcgaacga cgacggcgcc gccttccgcc
     8461 aggccggcgt caaactggcc gcagccgccc tccaagaggg gcgcgacgaa gcgcgccgcc
     8521 tcctcgaagc cggcggcaaa ggcatggcct gcgccgagcg gctcagcgaa ttgcaggacg
     8581 acgttatcgc cgcgatccat gcgctggcct tgcgcgaaat tcgcggcgac cagccgggcg
     8641 agggcgacca gccgatggcg atcgccgcag tcggcggcta tgggcgcggc gcgctcgccc
     8701 ccggctccga tatcgatctg ctcttcttgt tgccgaccaa gccgacgccg ggcagcgaga
     8761 agctggtcga gacgatgctc tacatgctgt gggacttgcg ccagaaggtc ggccattcga
     8821 cgcgctcgat cggcgagtgc atgcgccagg ccaaggccga catgacggtc cgcacctcgc
     8881 ttttggaggc gcgcttcatt ctcggcgacg aagccttgtt cgaggagatg cgcaaaaggt
     8941 tcgacgccga gatcgtcgcc aaatcggctg aggatttcgt cgccgccaag ctcgccgagc
     9001 gcgacgagcg cttaaggcgc gccggcggct cgcgctacct cgtcgagccc aacgtcaagg
     9061 acggcaaggg cggcatccgc gacctcaaca ctttgttctg gatcggcaaa tacgtgtacc
     9121 gcgtgcgcca tgcgcgcgac ctcgtcgagg ccggcttgtt cgacgcccgc gaatatgcgc
     9181 tgttccggcg ctgcgaggaa ttcctgtggg cggtgcgctg ccacctccat ttcctcgccg
     9241 gccgcgccga ggaccgtctg ggctttgaat tccagccggc gatcgccgag cggcttggct
     9301 acatgtcgcg tttcggcctg accgaggtcg agcgtttcat gaaacattat ttcctcatcg
     9361 ccaaggatgt cggcgacctc accgccattg tctgcgccgc gctcgaagag cgccaggcca
     9421 agccgcgcgc cgcgctcgac cggttcatcg gccggctgcg ccgccgcgcc cgcatcgtcg
     9481 ccggcgccgc cgatttcatc gtcgaccatg gccgtatcag cgtcgtcaac gcccgcgttt
     9541 tcgagcgcga tccgatcaat ctcatccgcc tctattgggc cgccgaccgc tccggcctcg
     9601 ccgttcatcc cgatgcgacg cgcctcgtca cgaaatccct gaatcggatc gacgccaagc
     9661 tgcgcaacga tcccgaggcc aatcggctgt ttctcgaagt gctcacctcg cgccgcgcgc
     9721 cggaaaccgt gctgcggctg atgaacgaag ccggcgtgct cggccgcttc attcccgatt
     9781 tcggccgcat cgtcggcatg atgcaattca gcatgtacca ccactatacg gtggacgagc
     9841 atttgctgcg cacgatcggc gttctggcgg gcctgcgctc cggcgcgctg gtcgaggagc
     9901 atccgctggc cagcgagttc ctgcccggca tcgccaatcc ctccctgctt tatgtcgcgg
     9961 cgttcctgca cgacatcgcc aagggcgggc cggaggatca ttcgatcgcc ggcgccgcgg
    10021 tcgcccggcg gctctgcccg cggctcggcc tctcgccggg cgagaccgaa acggtcgcct
    10081 ggctggtcga gcagcatctg gccatgtcga tgaccgcgca gagccgcgac ctttccgatc
    10141 cgcgcaccat cgaagccttc gccgccattg tgcagacgct ggagcggctg cgcctgctgc
    10201 tggtcctgac gatttgcgac atccgggcgg tcggccccgg cgtctggaat ggctggaagg
    10261 gccaattgct gcgcacgctc ttctgggaga ccgaggtcgt gctgagcggc ggccattcgg
    10321 cggccgaccg caaggcgcgc gtcgccgaat cgcaggaggc gctgcgccag gcgctgcccg
    10381 gctggtccga tcccgaattc gacgcttacg cggcccggct ctatccggcc tattggctgc
    10441 gagtcgacct gccgcaccgc gtcgcccacg ccaagctgct tcacgccatg gccaaggaaa
    10501 cgcgctcgct gtcgacggaa ttttcaaccg acgcgttccg cggcgccacc gaattgacgg
    10561 tggtcgcgcc cgatcatccg cggctcctgg cggtcattgc cggcgcctgc gccgcttccg
    10621 gcggcaacat cgttgacgcc cagattttca ccacgacgga cgggctggcg ctcgacacga
    10681 tttcgctgag ccgcgccttc gaccgcgatg aggacgaatt gcgccgcgcc ggccgcatcg
    10741 ccaaaggcgt cgaggacgcc ttgcgcggca aaattcggct gaccgacatc atcgccgcca
    10801 agcgcaagcc ggacccccgg gcaggggcct tcaccgtcgc gccggaagtg acaatcagca
    10861 attccttgtc gaatcgcttc accgtgatcg aaatatccgg ccttgaccgg cccggcttgc
    10921 tctatgaggt gaccagcgcg ctttccaatc tcaatttgaa catcggctct gctcatatcg
    10981 ttaccttcgg cgaaaaagcc gtcgatacgt tttacgtgac cgacctgacc ggctcgaaaa
    11041 tcgcctcgcc ggcccgccag gcggcgatca agcgccacat gctggcgatt tttgacggcc
    11101 cggacgagaa aaagccggcg gccggcggcc gttgaggcgc ttgatttttg caccgcaaga
    11161 actgatatcc cgccacgaca aatccaccgt tccaagacgc gacatggacg accccgtgaa
    11221 aaaagacaaa gacgaggccg cgacggcgcc gctttcgggc gatggcggcg ccaatgccgc
    11281 cgaacaggcc gcccccgatc cggcagcggt tatcgagacc ctcaacgccg aaaacgcctc
    11341 gctcaaagac cgcctgctgc gcgccatggc ggagatggag aatctgcgcc gtcgcaccga
    11401 gcgcgaaatc gccgacgcaa agacctatgg cgtcgcctct ttcgcccgcg acatgctgac
    11461 cgtcgcggac aatctcggcc gggccctcga cagcctgccc gaagaggcgc gcgccggcgg
    11521 cgccgagggg gcgctgaaag gcctgatcga aggcgtcgaa ttgacgatgc gggacttgac
    11581 cgccgcgctc gcccgccatg gcgtcaagaa gcttgaaccg ctgggcgaga aattcgatcc
    11641 caatttccat gaggcgcttt atgaggcgcc caacgaggag ctgcccgccg gcacggtggc
    11701 gcaggtcgtc cagagcggct ggcggattgg cgaccgcgtt ctgcgcccgg cccgggtcgg
    11761 cgtctccaag ggcggcccga aagtggcggc gaaggcggag tgatttcggc gccgcgcgat
    11821 ctgtagccgc gcgacgagcg cggcgcgggg gagggcgggc gctgtcgccg accgcgccta
    11881 caaggggcga gcccctcacc ctcgcgtcac cctgcgcagc gtcagattga tccgcccgcc
    11941 gcccgcaagc agcgtcgacg agccgccgcg aatccggtcc acgccgtgaa aggcgaggcg
    12001 ggccggcccg cccaggatca gcgcgtcgcc ggagttcagc tcgaacgagc gcgtcctgtc
    12061 gccccgtttc gacccgccga cccggaacag gcaggaatcg ccgagcgaca gagacaggac
    12121 cggcgcggag aaatcctcct cgtcgcggtc ctgatgcagc cccatccggg cggacgcgtc
    12181 atagacgttg accaggcagg cttccggcgg cgccgcgccc gtggcgagat cggcccaggc
    12241 ggcgagcgcg atcgccggga tcggcggcca cggcgcgccg gtcgccggat gcgccggctg
    12301 gtagcgatag ccggcctcgt ccgagaccca gccgagcggc ccgcaattgg tcattcggac
    12361 ggagaacggc ttgcccgtcc gcggcatgcg cggcgtgaag agcggcgcgg cgcgcagggc
    12421 ctcgcccaac gcgtcgcgca gagcttcctg cgccggccgg tcgagatagg ccgggtaatg
    12481 gacgaggcct gggatgattt cgagtccgcg ggcgggagcg gtcatagccc ccgcttaccc
    12541 tggtcggccg gcgcggccaa gcgcccggcg tcagaggctt gcgagattgc agtttagctc
    12601 catttctgct ccaatacgga gctgtatcgg attatgctca tggacctgtc gatcaaagcc
    12661 gtgccggaag agcaagtgct gaagctccgc gcccgggct
//
LOCUS       DATGJW010000356         6955 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1515548, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000356 DATGJW010000000
VERSION     DATGJW010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6955)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6955)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6955
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1515548"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(171..929)
                     /locus_tag="VKU03_10840"
     CDS             complement(171..929)
                     /locus_tag="VKU03_10840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YggS family pyridoxal phosphate-dependent
                     enzyme"
                     /protein_id="HLJ71806.1"
                     /translation="MSSSAQAITSVTDRLAALRARIERRARDCGRDPASVRLVAVSKT
                     FPADEVWPAIAEGGQFLFGENRVQEAKVKWRILRDRAAALGKTIDLHLIGPLQSNKAR
                     EAVEIFDAIETLDREKLAAALAEAIAKTGRRPRLFVQVNTGAEPQKAGVAPQEADAFI
                     ALARETYGLAIDGLMCIPPVDEQASPHFALLGEIAKRNGLKELSMGMSADFELAIQLG
                     ATYVRVGSAIFGARDRDNPPRPAQRGEGGDEAAG"
     gene            1121..1513
                     /locus_tag="VKU03_10845"
     CDS             1121..1513
                     /locus_tag="VKU03_10845"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLJ71807.1"
                     /translation="MPRPESFVKRVEDVEPEAWSDARGRLSFHTLISGDLTPSEGLVA
                     GVAIVEPGGELAAHAHAPPEIYFALDGAALVTIAGVERKVSAGAAVYIPSDAVHSIRN
                     PFDRAFRIFYAFPADRFDAIEYKFAAGV"
     gene            complement(1516..1905)
                     /locus_tag="VKU03_10850"
     CDS             complement(1516..1905)
                     /locus_tag="VKU03_10850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mth938-like domain-containing protein"
                     /protein_id="HLJ71808.1"
                     /translation="MSEPGGYVPGRRSIEAYGAGGFRFAGMSHRGSILATPAGVRAIA
                     PLSFAELDAETLRPLFDEMAANPRSIEILVIGTGEKLIPLGGELRDRLRKAGLRFEAM
                     ATGPAARMYNVLIAEERRVAAVLIAAP"
     gene            complement(1910..2860)
                     /gene="secF"
                     /locus_tag="VKU03_10855"
     CDS             complement(1910..2860)
                     /gene="secF"
                     /locus_tag="VKU03_10855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecF"
                     /protein_id="HLJ71809.1"
                     /translation="MKLLRLAPENTKFGFMRFRRVSYPLSATLSIISVLVFFFVGMNF
                     GIDFSGGTQVMLRAKHGAADIAELRKVGESLGLGQVEVQRIGPLTDAVVRVGVQPGGE
                     AGQQNAVNLMRRAFGNDYDFRSVDEVGPRVSGELVQKGTLGIVVSIIAVLTYLWFRFE
                     WQFAVGAIIGTMHDLLLTVGFFCVTQLEFNSASIAAILTIVGYSLNETVVVLDRIREV
                     MRKYKRLSTAEIVDMSVNAVLPRTIMTATTVFLALLALFFLGGEVIRSFSAAMIWGIF
                     VATYSSIFICSPVLIYLGVRHDVFDKAPAQGEAGAADAKA"
     gene            complement(2967..4556)
                     /gene="secD"
                     /locus_tag="VKU03_10860"
     CDS             complement(2967..4556)
                     /gene="secD"
                     /locus_tag="VKU03_10860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HLJ71810.1"
                     /translation="MLRFAPWRVASIVALVVAAILLVVPSFLSPERVAALQKSLPSIL
                     PARQIVLGLDLQGGAHVLLEVDSASLTHAQVEQLRDDVRGKLREGRISIAGGIAMQQR
                     GVQVRIADAAEREKALQLLRQLNQPIGNAVIGASGHTLDINQTDQGAVQVTLTDAAIK
                     DKIRRAVSQSIEVLRRRVDALGTTEPNIQQEGSDRVLVEVPGLQDTTRLKQILGTTAK
                     LEFRLVAQPGEPPNEVEELPQQNGGPPIPVEKRVMVQGEDLVDAQASFDQNGQPDVNF
                     RFNLRGGQRFGQVTSENVGRPFAIVLDGKVISAPRILGPITGGSGQITGNFSVEEADN
                     LAVLLRAGALPAKLTVVEERTVGPGLGQDSIDAGKRAAMVGGALVVIYMLTTYGIFGV
                     FADLALGVHILFIFASMALLGATLTLPGIAGVVFTIGMAVDSNVLIYERIREESHLGR
                     SVISSLDAGFRRAFATIIDSNVTMFVAAVILYLFGSGPVRGFAVSLGLGILTSVITAV
                     TMTRMMIALWYRYRRPVKLPI"
     gene            complement(4611..4955)
                     /gene="yajC"
                     /locus_tag="VKU03_10865"
     CDS             complement(4611..4955)
                     /gene="yajC"
                     /locus_tag="VKU03_10865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483888.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HLJ71811.1"
                     /translation="MITPAFAQAAGGTAGPQDMLIQFAPFVVIFAIMYFLILRPQQRR
                     AREHKEMLKGARRGDTIVTTGGLIGKITKATDDGEVEVEIAPNVRVRIARSGIGEVRA
                     RGEPVKEAVGKS"
     gene            complement(5152..>6955)
                     /gene="katG"
                     /locus_tag="VKU03_10870"
     CDS             complement(5152..>6955)
                     /gene="katG"
                     /locus_tag="VKU03_10870"
                     /EC_number="1.11.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018117166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004096 - catalase activity [Evidence
                     IEA];
                     GO_function: GO:0004601 - peroxidase activity [Evidence
                     IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="catalase/peroxidase HPI"
                     /protein_id="HLJ71812.1"
                     /translation="NLDKARRLLWPIKQKYGRKISWADLMILAGNVALDSMGFKTFGF
                     GGGRADVWEPEELYWGPEGTWLGDERYSGERELQHPLAAVQMGLIYVNPEGPNGKPDP
                     IAAAKDIRETFFRMAMNDEETVALIAGGHTFGKTHGAGDPSFVGPAPEAAAIEDQGLG
                     WKSRHGAGFGPDAITGGPEVIWSQTPTKWSNHFFDNLFNNEWELTKSPAGAWQWQAKG
                     AAATIPDAHDKSKKRVPTMLTTDLSLRFDRAYEKISRRFYEHPDQFADAFARAWFKLT
                     HRDMGPIQRYLGPLVPKESLIWQDPIPAVDHPLIGEADIAALKAKILASGLSVSELVS
                     TAWASASTFRSSDKRGGANGARIRLAPQKDWEVNQPAQLAKTLQRLEAVKEAFNAAQT
                     GGKRVSLADLIVLGGAAAIEKAAKAAGCEATVPFTPGRMDASQAQTDVESFAPLEPLA
                     DGFRNYLSGRQFMAPEEALVDRAQLLTLTAPEMTVLVGGLRVLGANAGGSPHGVFTKR
                     REALTNDFFVNLLDMGTEWRPSAGAEGVYEGRDRKTGEAKWTGTRVDLIFGSHSQLRA
                     FAEVYACADSKEKFVKDFVAAWSKVMNLDRFDLA"
BASE COUNT         1200 a         2263 c         2325 g         1167 t
ORIGIN      
        1 ccaacggtcg gtcgggctat ggccgcttga cgatgccggg ccgatgtggc cgcgcgccaa
       61 gatgcgtgca tgccgtagcg ccttgcggag aaggtggcgc gcagcggcgg cgctgcactc
      121 gaaaggtacg cgccgaggcg ctaaccggca ggttccgcac tgccccttgc tcacccggcc
      181 gcttcgtcgc cgccctctcc ccgctgcgcg gggcgagggg gattgtcccg atcccgagcg
      241 ccgaatatcg cgctgccgac ccgcacatag gtcgcgccga gctggatcgc cagctcgaaa
      301 tcggcgctca tgcccatcga gagttccttg agcccattgc gcttggcgat ctcgccgagc
      361 aaggcgaaat gcggcgaggc ctgctcgtcg acgggcggga tgcacatcag gccgtcaatg
      421 gcgaggccgt atgtctcacg cgccagcgcg atgaaggcgt cggcttcttg cggcgcgacg
      481 ccggcctttt gcggctccgc gccggtattc acctgcacga aaaggcgcgg gcggcggccg
      541 gtcttggcga tggcttcggc aagcgctgcg gcaagcttct cgcggtcgag cgtttcgatc
      601 gcgtcgaaaa tctcgaccgc ctcgcgcgcc ttgttgctct gcaaggggcc gatcagatgc
      661 aaatcgatcg tcttgccgag cgcggcggcg cgatcgcgca aaatccgcca tttgaccttg
      721 gcttcctgaa cgcgattctc gccgaacaga aattggccgc cttcggcgat ggccggccag
      781 acttcgtcgg ccgggaaagt ctttgagacg gcgacgagac gaaccgaggc cggatcgcgg
      841 ccgcaatcgc gggcgcggcg ctcgatccgc gcgcgcaacg ccgcgaggcg atcggtgacg
      901 ctcgttatcg cttgcgcgct tgacgacatg gagacttgcc ttcttccgca tctatcattc
      961 ccacgcaagc gggaatccaa gcgggtcagt cctgaggcgg aggctcctcg attcccgctt
     1021 tccgcgggaa tgacatcggg caaaacagcg attccatcaa gacgcgcgcc gcgctagcct
     1081 gctctctgat ttcgttttga gttcgggaag gggcgttcgc atgccgagac cggaatcttt
     1141 cgtcaaacgg gtcgaggacg tggagcccga agcttggagc gacgcgcgcg gccgactgtc
     1201 gttccacacg ctgatcagcg gcgacttgac gccgagcgaa ggtctggtgg ccggcgtcgc
     1261 catcgtcgag ccgggcggcg aactggcggc gcatgcccac gcgccgccgg aaatctattt
     1321 cgcgctcgac ggcgccgccc tcgtcacgat cgccggcgtc gagcggaaag tctcggccgg
     1381 cgccgccgtc tacattccga gcgacgcggt tcattcgatc cgcaatccct tcgatcgggc
     1441 gttcaggatt ttctacgcct tcccggccga ccggttcgac gcaatcgagt acaaattcgc
     1501 cgcaggcgtt tagcgtcatg gcgcggcgat cagcaccgcc gcgacgcgcc gctcctcggc
     1561 gatcagcacg ttatacatcc gcgccgccgg gccggtcgcc atcgcctcga agcgcagccc
     1621 ggccttgcgc agccggtcgc gcagctcgcc gccgagcggg atgagcttct cgcctgttcc
     1681 gatcaccagg atttcgatcg agcgtggatt ggcggccatt tcgtcaaaga gcggccgcag
     1741 cgtctcggcg tcgagctcgg cgaagctcag cggcgcgatc gcgcgcacgc cggccggcgt
     1801 ggccagaatc gagccgcgat gcgacatgcc ggcgaaacgg aagccgccgg cgccataggc
     1861 ttcgatcgag cgccttcccg gaacgtagcc gccgggctcg ctcaattctt taggccttcg
     1921 cgtcggccgc gccggcctct ccctgggcgg gcgccttgtc gaacacgtcg tgccgcacgc
     1981 cgagatagat caaaaccggc gagcagatga agatcgacga gtaggtggcg acgaaaatgc
     2041 cccagatcat cgccgccgag aaggagcgaa tgacctcgcc gccgaggaag aacaaagcga
     2101 gcagcgccag gaacaccgtc gtcgcagtca tgatcgtgcg cggcagcacg gcgttgaccg
     2161 acatgtcgac gatctcggcg gtcgacagac gcttatattt gcgcatcacc tcgcggatgc
     2221 ggtcgagcac cacgacggtc tcgttgagcg aatagccgac aatggtcagg atcgcggcga
     2281 tcgacgccga attgaattcg agctgcgtga cgcagaaaaa gccgaccgtg agcaagagat
     2341 cgtgcatcgt gccgatgatc gcgccgaccg cgaattgcca ttcgaagcgg aaccagagat
     2401 aggtcaggac ggcgatgatc gagacgacga tgccgagcgt gcctttctgc accaattcgc
     2461 cggagacgcg cggtccgacc tcgtcgacgc tgcgaaaatc atagtcgttg ccgaaggctc
     2521 ggcgcatcag gttgaccgcg ttctgctgcc ccgcttcgcc gccgggctgc acgccgacgc
     2581 ggaccacggc gtcggtcagc ggcccgatcc gctgcacctc gacctggccg aggccgagcg
     2641 attcgccgac tttgcgcaat tcggcgatgt cggcggcgcc gtgcttggcc cgcagcatca
     2701 cctgggtgcc gccggaaaag tcgatgccga aattcatccc gacaaagaag aagaccagca
     2761 ccgagatgat cgagagcgtc gccgagagcg ggtagctcac gcgcctgaag cgcatgaagc
     2821 cgaacttggt gttctcgggg gcgaggcgaa gaagtttcat gacgcaaacc tttcggcgtc
     2881 ggcggccccc tcccgtaccc gctcccgctg cgcgggagag gagggcctcg gcgccagcgg
     2941 cgcccgcaaa taaaccaaag cggcgctcat atcggcagct tgaccgggcg gcgatagcgg
     3001 taccagagcg cgatcatcat gcgggtcatg gtcacggcgg tgatgaccga ggtcaaaatg
     3061 ccgagaccga gcgagacggc gaagccgcgc accgggccgg agccgaaaag atagagaatg
     3121 accgcggcga cgaacatggt gacgttggag tcgatgatcg tcgcgaaggc ccgcctgaag
     3181 ccggcgtcaa gcgaggagat gacggagcgg ccgagatgcg attcctcgcg gatgcgctcg
     3241 tagatcagca cgttggaatc gaccgccatg ccgatcgtga agacgacgcc ggcaatgccg
     3301 ggcaaggtca gcgtcgcgcc gagcagggcc atcgaggcga agatgaagag gatatgcacg
     3361 ccgagggcga gatcggcgaa aacgccgaaa atgccgtaag tggtcagcat atagatcacg
     3421 accagggcgc cgccgaccat cgcggcgcgc ttgccggcgt cgatcgaatc ctggccaagg
     3481 ccgggcccga cggtgcgctc ctcgacgacg gtcagcttcg ccggcagcgc gccggcgcgc
     3541 agcagcacgg ccagattgtc ggcctcctcg accgagaaat tgccggtgat ctggcctgag
     3601 ccgccggtga tcggccctaa aatgcgcggc gccgaaatga ctttgccgtc gagcacaatg
     3661 gcgaagggcc ggccgacatt ctcgctcgtc acctggccga agcgctggcc gccgcgcaga
     3721 ttgaagcgga aattgacgtc gggctgaccg ttctggtcga agctcgcctg ggcgtcgacc
     3781 aggtcctcgc cctgcaccat cacccgtttt tcgaccggga tcggcgggcc gccattctgc
     3841 tgcggcaatt cctcgacctc gttcggcggc tcgcccggct gggcgacgag gcggaattcc
     3901 agcttggcgg tggtgccgag aatctgcttg agccgggtcg tgtcctgcaa gcccggcacc
     3961 tccaccagaa cgcggtcgga gccctcctgc tggatgttcg gctcggtcgt gccgagcgcg
     4021 tcgacgcggc ggcgcaagac ctcgatcgac tgcgagacgg cgcggcggat tttatccttg
     4081 atcgccgcgt cggtgagcgt cacctggacc gcgccctggt cggtttggtt gatatcgagc
     4141 gtatggcccg acgcgccgat cacggcgttg ccgatcggct ggttgagctg gcgcaggagc
     4201 tgcagggctt tctcgcgctc cgcggcgtcg gcgatcctca cctgtacgcc gcgctgctgc
     4261 atggcgatgc cgccggcgat ggagattctt ccttcgcgca gcttgccgcg gacatcgtcg
     4321 cgcaattgct cgacttgcgc atgggtgagc gacgcgctgt cgacttcgag cagcacatgc
     4381 gcgccgcctt gcagatcgag cccgagcacg atctggcggg ccggcaggat ggacggcagg
     4441 ctcttctgca aggcggcgac gcgctccggc gagaggaagc tcggcacgac aagaagaatg
     4501 gcggcgacga ccagcgcgac gatggaggcg acccgccagg gggcgaaacg aagcattgga
     4561 ctagtcggtc tccacgcgca tgctctttaa cgacgggcgg cggcttaagg tcacgatttg
     4621 ccgaccgctt ccttgaccgg ctcgcccctg gcccggacct cgccgatgcc ggaccgggcg
     4681 atgcgcacgc gcacattggg cgcgatctca acctcgactt cgccgtcgtc ggtcgccttg
     4741 gtgatcttgc cgatcaggcc gccggtcgtg acgatcgtat cgccgcgccg cgcgcccttg
     4801 agcatctcct tatgctcgcg ggcgcggcgc tgctgcgggc gcaggatcaa gaaatacatg
     4861 atcgcgaaaa tgacgacgaa aggcgcgaat tggatgagca tatcctgcgg cccggccgtg
     4921 ccgcccgcgg cctgggcgaa tgctggcgta atcagcaaag attgaactcc gactggcccg
     4981 gcagcccgcc ggggccctca taaggggctt caagcggggc ggaatatagc ggcggtcgcc
     5041 gcgaaagcaa tcgggcttaa aagcgcgcag gcggcccggg ggccaatgcc cccggcgcgt
     5101 cgcggcgatt cgcgttgggt ctcgaagacc ttgaaatcct tggcttcggg atcaggcgag
     5161 atcgaacctg tcgaggttca tcaccttgct ccacgccgcc acgaagtcct tgacgaactt
     5221 ctccttcgaa tccgcgcagg catagacttc cgcgaaagcc cggagctgcg agtgcgatcc
     5281 gaaaattaaa tcgacgcgcg tgccggtcca cttggcctcg ccggtcttgc ggtcgcgccc
     5341 ctcatagacg ccctcggcgc cagcggacgg ccgccattcc gtgcccatat cgagcagatt
     5401 gacgaagaag tcgttggtca gcgcctcgcg ccgcttggtg aagacgccgt gcggcgagcc
     5461 ccccgcattt gcgccgagaa cgcgcaggcc gccaacgagg accgtcattt cgggcgcggt
     5521 cagcgtcagc aattgcgccc ggtcgaccag cgcctcctcg ggcgccatga actgcctgcc
     5581 gctgagataa ttgcggaagc cgtcggcgag cggctcgagc ggggcgaagg attcgacgtc
     5641 ggtttgcgcc tgcgaggcgt ccatgcggcc cggcgtgaac gggaccgtcg cctcgcagcc
     5701 ggcggccttc gcggccttct caatcgcggc ggcgccgccg agaacgatca gatcggcgag
     5761 cgagaccctc ttcccgccgg tttgcgcggc gttgaacgcc tccttcaccg cttcgagcct
     5821 ttgcagcgtc ttggccaact gagccggctg attgacctcc caatcctttt gcggcgcaag
     5881 gcgaatgcgc gcgccattgg cgccgccgcg cttgtcggag ctgcggaagg tcgaggccga
     5941 cgcccaggcg gtcgagacaa gctcggaaac cgagaggccg gaggcgagga ttttggcctt
     6001 cagcgcggcg atgtccgctt ccccgatcag cggatgatcc acagcgggaa tcgggtcttg
     6061 ccagatcagg ctttccttgg ggacgagcgg gccgagataa cgctggatcg ggcccatatc
     6121 gcgatgcgtg agcttgaacc acgcccgggc gaatgcgtcg gcgaactgat cgggatgctc
     6181 gtagaagcgc cgcgatatct tctcataggc ccgatcgaag cgcagcgaga gatcggtcgt
     6241 cagcatggtc ggcacgcgct tcttcgattt gtcgtgcgcg tccggaatgg tcgctgcggc
     6301 gcctttcgcc tgccattgcc aggcgccggc cgggctcttg gtcagctccc attcgttgtt
     6361 gaacaggttg tcgaagaaat ggttgctcca tttcgtcggc gtctgcgacc agatgacttc
     6421 cggcccgccg gttatggcgt cggggccgaa gccggcgccg tgcctgctct tccagccgag
     6481 gccctgatcc tcgatggcgg cggcctccgg ggccgggccg acaaaagacg gatcgccggc
     6541 gccgtgggtt ttgccgaatg tatggccgcc ggcgatcagc gcgaccgtct cctcgtcgtt
     6601 catcgccatg cggaagaacg tctcgcggat gtccttcgcc gccgcgatcg gatcgggctt
     6661 gccgttcggc ccttccggat tgacgtagat caggcccatt tgcacggcgg cgagcggatg
     6721 ctggagctcg cgctcgccgc tatagcgctc gtcgccgagc cacgttccct cgggacccca
     6781 atagagctct tcgggctccc agacatcggc gcggccgccg ccgaaaccga acgtcttgaa
     6841 gcccatcgaa tcgagggcga cgttgccggc gaggatcatc aggtcggccc aggagatttt
     6901 ccggccgtac ttctgcttga tcggccacaa gagccggcgc gccttgtcga ggttg
//
LOCUS       DATGJW010000357         2159 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_381056, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000357 DATGJW010000000
VERSION     DATGJW010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2159)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2159)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2159
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_381056"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..312
                     /gene="erpA"
                     /locus_tag="VKU03_10875"
     CDS             <1..312
                     /gene="erpA"
                     /locus_tag="VKU03_10875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012753315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster insertion protein ErpA"
                     /protein_id="HLJ71813.1"
                     /translation="ISASAARRLAKILSSEEPGAALRVSVKGGGCSGFQYSFDIDKSR
                     AGDDLVVERDGATILIDPVSLEYMAGAEVDFVDDLMGQAFRVKNPNAVASCGCGVSFS
                     V"
     gene            complement(322..1104)
                     /locus_tag="VKU03_10880"
     CDS             complement(322..1104)
                     /locus_tag="VKU03_10880"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71814.1"
                     /translation="MKRFVIVIVSLLSAAPACAATIVAAPVGPRETGAIVVSGTLKPG
                     DDEVFSKRLMQFPKGIVVFQGDGGDLQAAIKIGTAIRLKNYATLVPTDSNCASACAVA
                     WLGGAPRLMEDGSQIGFHAAYALKNGQTTEAGVPNALLGAYLGKIGLPDRAVVYITEA
                     PPSGMKWLSSADAEKMGIDVRTIAGPKPPAFSAPPSIAPEPTATRDQHVKSHPKRPKT
                     AHAGAKDKRYFGSWVMAALRKLGTSATEAKPAPPPGNAIRGR"
     gene            complement(1206..>2159)
                     /locus_tag="VKU03_10885"
     CDS             complement(1206..>2159)
                     /locus_tag="VKU03_10885"
                     /EC_number="2.7.1.90"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diphosphate--fructose-6-phosphate
                     1-phosphotransferase"
                     /protein_id="HLJ71815.1"
                     /translation="QEILKILKAHGIGAFFYIGGNDSSDTARIVAEEARKSGHPLRAM
                     HIPKTIDNDLVGNDHTPGYPSAARFVAQAFMGVNLDNAALKGVYCAVVMGRHAGFLTA
                     ASAMARQSPGDGPHLIYLPERPFAVDRFLADVKAAYDKHGRCIVAVSEGVHDEKGEPI
                     LAKLAAGGLEKDAHGNVQLSGTGALADLLCEEIKAKLGIKRVRGDTFGYLQRSFVGCV
                     SNVDQREAREVGERAVQFALRGEGDGSVAIKRVGDYAVDYQKVALESVAAKTRVMEDH
                     FIAPAGNDVTAAFRDYLRPLLGANMPEVARLRGIKVAKIGK"
BASE COUNT          359 a          700 c          707 g          393 t
ORIGIN      
        1 atcagcgcga gcgcggcccg ccggcttgcg aaaatcctgt cgagcgaaga gcccggcgcc
       61 gctttgcgcg tgtccgtgaa aggcggcggc tgttcgggct ttcaatattc cttcgatatc
      121 gacaagagcc gcgccggcga cgatctcgtg gtcgagcgcg acggcgcgac cattctgatc
      181 gatccggtgt cgctcgaata tatggccggc gccgaagtgg atttcgtcga cgatctgatg
      241 ggccaggcgt tccgggtgaa gaaccccaac gcggtcgcct cgtgcggctg cggagtgagc
      301 ttctcggtat aggcgcgcgc ctcaccgacc gcggatggcg ttgcccggtg gcggcgccgg
      361 ttttgcctcc gtcgcgctcg tcccaagctt gcgcaaggcg gccatcaccc aggagccgaa
      421 atagcgcttg tccttcgctc cggcatgcgc ggttttcggc cgcttcgggt ggcttttgac
      481 atgctgatcg cgagtcgcgg tcggttcggg agcaattgaa ggcggcgcgg agaaagccgg
      541 cggtttcggc ccggcgatcg tcctgacgtc gatccccatc ttctccgcgt cggcggagct
      601 gagccatttc atgccgctcg gcggcgcttc ggtgatgtag accacggcgc ggtccggcag
      661 cccgatcttg cccagatagg cgccgagcag agcgttggga acgccggctt ccgtcgtctg
      721 gccgttcttt aacgcatagg cggcgtggaa gccgatctgc gatccgtcct ccatcaggcg
      781 cggcgcgccg ccgagccaag cgacagcgca ggccgatgcg caattgctgt cggtcggcac
      841 gagggtcgcg taattcttca atcggatcgc cgtgccgatc ttgatggcgg cttgcagatc
      901 cccgccatcg ccctggaaga cgacgatccc cttcggaaac tgcatcaatc ttttggagaa
      961 gacctcgtcg tcgccgggct tcagcgttcc tgagacgacg atcgcgccgg tttcccgcgg
     1021 gccgaccggc gcggcgacaa tcgtcgccgc gcaagccggg gcggctgaca acagactgac
     1081 gattacaata acgaagcgct tcatcgctat cgccgggccc ggagacgcag attcgcaacc
     1141 gcgactctac ctgagctcag gcgtccaacc aaccgctcgg cgcgtcttat gaaccggcgc
     1201 gacgatcatt tcccgatttt ggccaccttg attccgcgca gccgagcaac ctcgggcatg
     1261 ttcgcgccga gcagcggacg aagatagtcg cggaacgcgg cggtcacgtc attgccggcc
     1321 ggcgcgatga aatggtcttc catcacccgc gtcttggcgg cgactgattc gagcgcgact
     1381 ttctgatagt cgacggcgta atcgccgacg cgcttgatcg cgaccgagcc gtcgccctcg
     1441 ccgcgcagcg cgaattgcac ggccctttcg ccgacctcgc gcgcctcgcg ctgatcgaca
     1501 ttcgagacgc agccgacgaa cgagcgctgc aaatagccga acgtgtcgcc gcgcacgcgc
     1561 ttgatgccga gcttcgcctt gatctcctcg cacaagagat cggcaagcgc gccggtgccg
     1621 gaaagctgga cattgccatg cgcgtctttt tcaagaccgc ccgccgccag cttggcgagg
     1681 atcggctcgc ccttctcgtc atggacgccc tcggacaccg cgacgatgca gcggccatgc
     1741 ttgtcatagg ccgccttcac gtcggcgagg aaccgatcga cggcgaaagg gcgctcgggc
     1801 agatagatga gatgcggccc gtctccgggc gactggcgcg ccatggccga ggcggcggtg
     1861 aggaagccgg catggcggcc catgacgacc gcgcaataga cgcccttcag cgccgcattg
     1921 tcgagattga cgcccatgaa ggcctgggcg acgaagcgcg cggcggacgg gtagccgggc
     1981 gtgtgatcgt tgccgacgag atcgttgtcg atcgtcttcg ggatatgcat cgcccgcaag
     2041 ggatggccgc tctttcgcgc ctcctcggcg acgatgcgcg cggtgtcgga ggaatcgttg
     2101 ccgccgatat agaagaacgc gccgatccca tgcgccttca ggattttgag gatttcctg
//
LOCUS       DATGJW010000358         8228 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_585901, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000358 DATGJW010000000
VERSION     DATGJW010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8228)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8228)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8228
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_585901"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(148..984)
                     /gene="kynA"
                     /locus_tag="VKU03_10890"
     CDS             complement(148..984)
                     /gene="kynA"
                     /locus_tag="VKU03_10890"
                     /EC_number="1.13.11.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004833 - tryptophan 2,3-dioxygenase
                     activity [Evidence IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan 2,3-dioxygenase"
                     /protein_id="HLJ71816.1"
                     /translation="MSDPYDPASEGAETSFESRMSYGDYLNLDDILGAQKPLTGAHDE
                     MLFIIQHQVSELWMRLAVHEIGAARKAVAGDALQPAFKMLSRIARIFEQLNNAWDVLR
                     TMTPSEYTHFRGELGQSSGLQSWQYRQIEFMVGNRNPAMLRPHAHRRDIVALLEAELS
                     RPSLYDEALRLIARNGIAVPKAVLERDLAEAYRMSDGVVAAWRVVYEDPERHWTLYEL
                     AEKLVDFDDYFRRWRFNHVTTVERVIGFKRGTGGTGGVSYLKHMLEVVLFPELWQMRT
                     VL"
     gene            complement(981..2174)
                     /gene="kynU"
                     /locus_tag="VKU03_10895"
     CDS             complement(981..2174)
                     /gene="kynU"
                     /locus_tag="VKU03_10895"
                     /EC_number="3.7.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006612899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030429 - kynureninase activity [Evidence
                     IEA];
                     GO_process: GO:0019441 - tryptophan catabolic process to
                     kynurenine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="kynureninase"
                     /protein_id="HLJ71817.1"
                     /translation="MTDFAATKPKFHLPQGIVYLDGNSLGPLPKGAMERARRTIEDEW
                     GETLIRAWNLAGWMDQPRRVGDKIAPLIGAAPGTVVMGDTLSIKMYQALAAALTMRPD
                     RKVVLSDTGNFPTDLYMAEGLIQSLARGHVLKTVAPEAVEAAIDESVAALMLTHVDYG
                     SGRMHDMARLTKKAHAAGAVAIWDLAHSAGALPIDLAVCKADFAAGCTYKYLNGGPGA
                     PAFIYVRPDHIETARPALSGWLGHATPFDFDLNYRPASGIERMRVGTPPVVQMSILEA
                     ALEIWDDVDLAALRARSIELSELFIREVEARCPALALASPRDPAMRGSQVSFRFEHGY
                     AAMQALIARGVIGDFRAPDRMRFGFAPLYIDASDVRRAVGILAGVLDGRAWDRPEYKR
                     REAVT"
     gene            complement(2171..3796)
                     /locus_tag="VKU03_10900"
     CDS             complement(2171..3796)
                     /locus_tag="VKU03_10900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006931405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLJ71818.1"
                     /translation="MLGPSAHTDTFARDRLPPPGQWPDFLLDGFEYPERLNAAVELTD
                     RMVEKGFADHTALIGNGRRRTYKELADWTNRIAHALTEDYGVKPGARVLIRSANNPAM
                     VACWLAATKAGAVVVNTMPMLRAGELAKIVDKAEISLALCDTRLMDELVACAKDSAFL
                     KQVIGFDGTANHDAELDRVALDKSVRFAAVETGRDDVALLGFTSGTTGEPKATAHFHR
                     DLLIIADGYAKEVLSVTPDDVFVGSPPLAFTFGLGGLAVFPLRFGAAATLLENASPPN
                     MIEIIQKYKATICFTAPTAYRAMLAAMDQGADLSSLRAAVSAGETLPAPVYEAWMAKT
                     GKPMLDGIGSTEMLHIFISNRFGDSRPGCTGKPVKGYEAKVVDENLNEVERGTIGKLA
                     VRGPTGCRYLADPRQANYVRSGWNLTGDSFWQDQEGYFHFAARSDDMIISAGYNIAGP
                     EVEAALLSHAAVVECAVIGAPDEERGEIVQAHIVLASGVERSAETAKRLQDHVKATIA
                     PYKYPRSIVFADALPKTATGKIQRFKLKREARA"
     gene            4022..4852
                     /locus_tag="VKU03_10905"
     CDS             4022..4852
                     /locus_tag="VKU03_10905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015343330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ71819.1"
                     /translation="MALAAISDWDDAYANTVHIPGGASYPALWSSLAKAFRDEIAARG
                     RAELDLAYGDGEREMLDLFRPPGAAKGLAVFAHGGYWLSFDKSSWSHLAAGALARGWT
                     VCVPSYPLAPQARIAAITRRFAAAVQFAAARMDGPLRLAGHSAGGHLVARMICEDSPL
                     PEHLRDRIAHVVSISGLHDLRPLLRTRMNATLGLDAAEAARESAALREPLAGRSIACW
                     AGGAERPEFIRQSRLLANIWTGLGAATEFALADGRHHFDVIDDLIDPESRLIKAWLGE
                     "
     gene            complement(4875..5918)
                     /locus_tag="VKU03_10910"
     CDS             complement(4875..5918)
                     /locus_tag="VKU03_10910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ71820.1"
                     /translation="MSQQSFLLRQQKSGGVLPIQPVPRAPRNFEDRDPAAPRLLSLAA
                     YDRRDQFDVWRTRLSAFWDTVPLSNDHAATGFTAEYVSANLGGVILNSGRFSAHALER
                     IARPASRSPLDHWSLVLGRSGEAWLDAGGRELRAGPGDMFLVSLDQEHRGQLSDYEGL
                     AMFLPRDALSDVAPALDSASATALSGPLAGLLADYLTDLEKRIIGMSEEELLTAGRAA
                     AAMVATCVQPSRDRLDAARPAIEAALFERGRAYIQAHLSNPDLGADLLLQELGVSRAS
                     LDRAFEHEGGVAHYIQRRRLHAARAALAANPNRLMQDIASSVGYRHAADFARAFRREF
                     GFSPSEARERGGP"
     gene            complement(5915..6202)
                     /locus_tag="VKU03_10915"
     CDS             complement(5915..6202)
                     /locus_tag="VKU03_10915"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71821.1"
                     /translation="MEETRVDWRAFFLVCAVQRETGSMRERYFKFLFNDEKISTAKAG
                     NPIILRQNRSTNASNQPFYSFDKSAKGAARRLARLRSAFDGGATSRIQARP"
     gene            6436..7410
                     /locus_tag="VKU03_10920"
     CDS             6436..7410
                     /locus_tag="VKU03_10920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012043204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0045027 - DNA end binding [Evidence IEA];
                     GO_process: GO:0006303 - double-strand break repair via
                     nonhomologous end joining [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Ku protein"
                     /protein_id="HLJ71822.1"
                     /translation="MPTARPYWKGYLKLALVSCPIALYTASSSSERVAFRQINKKTGN
                     RLRQQLIDEVTRQPVEAEDKGKGYEYAKGQYLAIEDEELESIEIESNHTIEIDSFVPR
                     AEIDERYLESPYYIAPNDPVGQEAFAVIREAMRGKAMVGLGRVVLAKRERIMMLQPWD
                     KGLMGTTLRYPYELRQPKDYFYDIPEIKVQQDLLKLAEHILKNKEQRFDPEQFVDRYE
                     EAVLEMLKKKQAGMPAPKAKAAFTPPSNVVNLFDAFRQSIAEEERTLAQPAKPVKAAK
                     PKKGKTKVEGQRELLLPIPGKKAAEAKPAKTEEAKPARAASSPRRKTG"
     gene            complement(7593..>8228)
                     /locus_tag="VKU03_10925"
     CDS             complement(7593..>8228)
                     /locus_tag="VKU03_10925"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HLJ71823.1"
                     /translation="GETSQKRATEGRAAAEPGNAGATGQNEAMAHGKMKMNAQQASDF
                     RSRLEKHGEVTRSSVNFNVNVGVAVPESVRLEPIPTDIIAEYPEFRGYDVVMVEDEIV
                     IVDPHTREVVEVIGGSTRSAAAAHRERLRLTREQDQVIRRHIHARPEKTVEFDERIDA
                     RVPDTVVLEPLPGPVVAEIPEVRSYDYFIDRSDHVVIVDPETHEVVGIVED"
BASE COUNT         1482 a         2662 c         2667 g         1417 t
ORIGIN      
        1 aacattgaag cagatggccc tggcgcgcta tggcgcggac atcgcgctgc ttcatttgtc
       61 cgagagacgg gtaatcgaaa tccccaaccc cctactggac ggccaagatc cgctcgatcg
      121 gcctgccttc tggtacaagc cctaacccta tagaaccgtc cgcatctgcc acagctcggg
      181 gaacagcacc acttcgagca tgtgcttgag ataggacacc ccgccggtcc cgccggtgcc
      241 gcgcttgaag ccgatgacgc gctcgaccgt ggtgacgtga ttgaaccgcc agcggcggaa
      301 atagtcatcg aaatcgacca gcttctcggc gagctcatag agcgtccaat gccgctccgg
      361 atcttcgtag acgacccgcc aggcggcgac gacgccgtcc gacatccggt aggcctcggc
      421 gagatcgcgt tcgagcacgg ctttcggcac ggcgattccg ttccgcgcga tcaggcggag
      481 cgcctcgtca tagaggctcg gccgcgacaa ctcggcttcg agcagcgcga cgatgtcgcg
      541 tctatgcgcg tgcggccgca gcatggcggg attgcggttg ccgaccatga attcgatctg
      601 ccggtactgc caggactgca gcccggacga ttggccgagc tcgccgcgaa aatgggtgta
      661 ttcgctcggc gtcatcgtcc gcagcacgtc ccaagcgtta ttgagctgtt cgaagattcg
      721 cgcgatgcga gacagcatct tgaacgccgg ctggagcgcg tcgccggcga cagctttgcg
      781 cgcggcgccg atctcgtgga cggcgagacg catccacaat tccgagacct gatgctggat
      841 gatgaacagc atctcgtcat gcgcgccggt cagcggcttc tgcgcgccga ggatgtcgtc
      901 gagattcaga tagtcgccgt aggacatgcg actctcgaac gaagtctcgg cgccttccga
      961 ggccggatcg tagggatcgc tcatgtgacc gcctcgcgcc gcttatattc cgggcgatcc
     1021 caggcgcgcc cgtcgagcac gccggcaaga atgccgaccg cgcgccgcac gtcgctcgcg
     1081 tcgatataga gcggcgcaaa gccgaagcgc attcggtccg gcgcgcggaa atcgccgatc
     1141 acgccgcgcg cgatcagcgc ttgcatcgcc gcatagccgt gctcgaaccg gaaggagact
     1201 tgcgagccgc gcatcgccgg atcgcgcgga ctggcgagcg ccagcgccgg gcagcgcgct
     1261 tcgacctcgc ggatgaacag ctcgctgagc tcgatcgagc gggcgcgaag cgcggccaga
     1321 tcgacgtcgt cccagatttc gagcgcggct tcgaggatgc tcatctggac gaccggcggc
     1381 gtgcccaccc gcatccgctc gatcccgctc gccggacgat agttgaggtc aaaatcgaac
     1441 ggcgtcgcat ggccgagcca gccggaaagc gccggccgcg ccgtctcgat atggtccggc
     1501 cgcacataga tgaaagccgg cgcccccgga ccgccattga gatatttgta ggtgcagccg
     1561 gcggcgaaat cggccttgca gacggcaaga tcgatcggca gcgcgccggc cgaatgagcg
     1621 agatcccaga tcgcgacggc gccggcggca tgcgccttct tggtcagccg cgccatgtcg
     1681 tgcattcggc cggagccgta atcgacatga gtgagcatca acgccgcgac gctctcgtcg
     1741 atcgccgctt cgaccgcctc gggcgcgacc gtcttcaaga cgtgaccgcg cgccagcgac
     1801 tggatcagcc cttccgccat atagagatcg gtcgggaaat tgccggtgtc cgacagcacg
     1861 accttgcggt cgggacgcat cgtcagcgcc gcggcgagag cctggtacat cttgatcgac
     1921 agcgtatcgc ccatcaccac cgtcccgggc gcagcgccga tcagcggcgc gatcttgtcg
     1981 ccgacgcggc gcggctggtc catccagccg gcgagattcc aggcgcggat cagcgtctcg
     2041 ccccattcgt cctcgatcgt ccgccgcgcc cgctccatcg cgcctttcgg cagcgggccg
     2101 agcgaattgc cgtcgagata gacgatcccc tgcggcagat gaaacttggg cttggtcgcg
     2161 gcgaaatcgg tcacgcccgc gcctcccgct tcagtttgaa gcgttgaatt ttcccggtcg
     2221 cggtcttcgg cagcgcgtcg gcgaagacga tcgagcgcgg atatttgtaa ggcgcgatcg
     2281 tcgccttgac gtggtcttgc aagcgcttcg ccgtctcggc gctgcgctcg acgccggaag
     2341 cgagcacgat atgcgcctgc acgatctcgc cgcgctcctc gtccggcgcg ccgatcaccg
     2401 cgcattcgac gaccgcggcg tgcgatagca aggcggcctc gacctcgggc ccggcgatgt
     2461 tgtagccggc cgagatgatc atgtcgtcgc tgcgcgcggc gaaatggaaa tagccttcct
     2521 ggtcctgcca gaaggaatcg ccggtcaaat tccagccgct gcgcacataa ttcgcctggc
     2581 gcggatcggc gaggtagcgg cagccggttg gaccgcgcac ggcgagcttg ccgatcgttc
     2641 cccgctcgac ctcgttcagg ttttcgtcga cgaccttggc ctcatagcct ttgaccggct
     2701 tgccggtaca gcccggccgg gaatcgccga agcgattgga gatgaagata tgcagcatct
     2761 cggtcgagcc gatgccgtcg agcatcggct tgccggtttt cgccatccag gcctcgtaga
     2821 ccggggccgg cagcgtctcg ccggccgaga cggcggcgcg cagcgaggaa agatcggcgc
     2881 cttgatccat agccgccagc atggcgcgat aggcggtggg cgcggtgaag cagatcgtcg
     2941 ccttatattt ctggatgatc tcgatcatgt tcggcggcga ggcgttctcc agcaaggtcg
     3001 ccgccgcgcc gaagcggagc ggaaagacgg cgaggccgcc aaggccgaag gtgaaggcga
     3061 gcggcggcga accgacgaag acgtcgtcgg gcgtcacgct cagcacctcc ttggcgtaac
     3121 cgtcggcgat gatgagcaag tcgcggtgaa aatgcgccgt cgccttcggt tcgccggtcg
     3181 tgcccgaagt gaagccgagc aaagccacgt cgtcccgtcc ggtctcgacc gcggcgaagc
     3241 gcaccgactt gtcgagcgcg acgcggtcga gctcggcgtc gtgattggcc gtgccgtcga
     3301 agccgatcac ttgtttgagg aaggcgctgt ccttggcgca ggcgacgagc tcgtccatca
     3361 gccgcgtgtc gcatagcgcc agcgagatct cggctttgtc gacgattttc gccagctcgc
     3421 cggcgcgcag catcggcatc gtgttgacga cgaccgcgcc ggccttcgtc gccgcgagcc
     3481 agcaggcgac cattgccgga ttgttggcgg agcggatcag cacgcgcgcg cccggcttga
     3541 cgccataatc ctcggtcagc gcatgggcga tgcggttggt ccagtcggcg agctccttgt
     3601 aagttcggcg ccggccgttg ccgatcagcg cggtatgatc ggcgaagcct ttctcgacca
     3661 tgcgatcggt gagctcgaca gcggcgttga gccgctcggg atattcgaag ccgtcgagca
     3721 ggaaatcggg ccattgcccc ggcggcggca gcctgtcgcg cgcgaacgta tccgtgtgag
     3781 ccgatggtcc gagcatgtca gcctctcgat tcgacgttaa ggcgcatcgc cgttgcggcg
     3841 atcggctctc gatgaatgaa tggcgttcgc ggcgtcgcgc gcttggtcga aagcgcgaaa
     3901 gagaggccgc ctggacataa gatgcgcatt cgacgcatcc tcccttgcgc gaggcgacgg
     3961 agcctttagt agcccgcaag ccgtgttagt tcaagcttca aggttttcgg acgagcgacg
     4021 catggccctt gcagcaattt ccgattggga cgacgcctac gccaacaccg tccatattcc
     4081 gggcggcgcg agctatccag ctctttggtc ctcgctcgcc aaggcgttcc gcgacgagat
     4141 cgccgcccgg ggccgcgccg agctcgatct cgcttatggc gacggcgagc gggagatgct
     4201 cgatctattc cgaccgcccg gcgcggccaa aggcctcgcc gttttcgcgc atggcggcta
     4261 ttggctgtct ttcgataaat cgtcctggtc gcatcttgcc gccggcgcgc tggcgcgcgg
     4321 ctggacggtg tgcgtgccga gctatccctt ggcgccgcag gcgcgcattg cggcgatcac
     4381 gcggcgcttc gccgccgccg ttcaattcgc cgcagcgcga atggatggac cgcttcgcct
     4441 cgccggccat tccgccggag gccatctcgt cgcgcgcatg atctgcgaag attcgccgct
     4501 gccggaacat ttgcgcgatc ggatcgcgca tgtcgtttcg atcagcggcc tccacgacct
     4561 gcgcccgctg ttgcgcacgc gaatgaacgc gacgctcggc ttggatgcgg ccgaggcggc
     4621 gcgcgaaagc gccgctctcc gcgaaccctt ggcggggcgc tcgatcgcct gctgggccgg
     4681 cggcgcggaa cggccggaat tcatccgcca gagccggctc ctcgccaata tctggaccgg
     4741 gcttggcgca gcgaccgaat tcgcgctcgc ggatggacgg catcatttcg acgtcattga
     4801 cgacctgatc gaccccgagt cgcgcttgat caaggcttgg ctcggcgaat aggacgcgcc
     4861 gcgtccggcc gtcgctatgg accgcccctc tcgcgcgctt cgcttggcga aaagccgaat
     4921 tcgcggcgga acgcgcgggc gaaatcagcc gcgtggcggt agccgacgct cgacgcgatg
     4981 tcctgcatga gccggttcgg attggcggcg agcgcggcgc gggcggcatg gagccgccgg
     5041 cgctggatgt aatgggcgac gccgccttca tgctcgaagg cgcggtcgag actcgctcga
     5101 gagacgccaa gctcttgcag gaggagatcg gcgccgagat cgggattgct caaatgcgct
     5161 tggatatagg cgcgaccgcg ctcgaacaac gccgcctcga tcgccgggcg cgcggcgtct
     5221 agccgatcgc gagagggctg aacgcatgtg gcgaccatgg ctgcggcggc gcggccggcg
     5281 gtgagcaatt cctcttcgct catcccgatg atgcgctttt cgagatcggt cagataatcg
     5341 gcgagaagcc ccgcgagcgg gccggaaagc gccgtggcgc tggcgctgtc gagcgccggg
     5401 gcgacgtcgc tgagggcgtc gcgcggcagg aacatcgcca gcccctcgta gtcgctcaat
     5461 tggccgcgat gctcctgatc aagcgagacg aggaacatgt cgcccggccc ggcgcgaagt
     5521 tcgcggccgc cggcgtcgag ccacgcttcc cccgagcggc cgagcacgag cgaccaatgg
     5581 tcgaggggcg agcggctcgc cgggcgcgcg atccgctcca gcgcatgcgc gctgaaccgg
     5641 cccgaattga ggatgacgcc gcccagattg gcgctgacat attcggcggt gaacccggtg
     5701 gcggcgtggt cgttcgacag cgggaccgtg tcccaaaaag cgcttagccg ggtacgccag
     5761 acatcgaatt ggtcgcgacg gtcataggcg gcgagcgaca gcaggcgcgg cgcggcggga
     5821 tcccggtcct caaaattccg cggcgcgcgc ggcaccggct ggatgggaag aacgccgccg
     5881 cttttttgct ggcgtagcaa gaagctttgc tgactcatgg tcgcgcctga atccttgatg
     5941 tcgcgccgcc gtcaaaagct gacctcagcc gcgcaagccg ccgtgcggcg cctttagccg
     6001 atttgtcgaa ggaataaaaa ggctgattgc tggcgtttgt ggagcgattc tggcgcaaga
     6061 ttatgggatt gcccgccttt gccgtagaaa ttttctcatc attaaataaa aatttaaaat
     6121 atcgctcccg catgcttccc gtttctcgct gaaccgcgca aacgaggaag aaggcgcgcc
     6181 aatcaacgcg cgtttcctcc aaagcgcgac gcgccggcga cacgttcaaa gtgaaaccga
     6241 ggcaaggcca cgtacgctca gaagcaagcc gaaacaaaat agaagccgag ggtctgatag
     6301 aagagcgccg cgtctcacaa agcattcgct ttgtagaacg cagcaagaac cttttacgac
     6361 gagaaaggac gtctattgac tctttttttg caaggatcag agtccatcgt ttcagcgttg
     6421 cgttaggagc cccgcgtgcc gaccgctcgt ccctattgga agggctatct caagctggcc
     6481 ctcgtatcct gcccgatcgc gctttacacc gcctcttcgt cgagcgagcg cgtcgccttc
     6541 cggcagatca ataagaagac cggcaatcgc ctgcgccagc agctcattga tgaggttacg
     6601 cgccaacccg tcgaggcgga ggacaagggg aaaggctacg aatacgccaa aggccagtat
     6661 ctcgcgatcg aggacgagga gcttgaatcc atcgagatcg aaagcaacca tacgatcgag
     6721 atcgattcct tcgtgccgcg cgccgagatc gacgagcgct atctcgagag cccttattat
     6781 atcgcgccga acgatccggt cggccaggag gctttcgccg tcatccgcga agcgatgcgc
     6841 gggaaggcga tggtcgggct tggccgcgtc gtgctcgcca agcgcgagcg gatcatgatg
     6901 ctccagccct gggacaaggg cctgatgggc acgacgctgc gctatcctta cgagctccgt
     6961 cagccgaagg actatttcta cgacatcccc gagatcaagg ttcagcagga tttgctcaag
     7021 cttgccgagc atatcctcaa gaacaaggag cagcgcttcg atcctgagca attcgtcgac
     7081 cgctacgagg aggcggttct cgaaatgctc aagaagaagc aggccggcat gcccgcgccc
     7141 aaagcgaaag ccgccttcac gccgccgagc aatgtcgtga acctgttcga cgccttccgg
     7201 cagagcatcg ccgaagagga gcggacgctc gcccaaccgg ccaagccggt caaggccgcc
     7261 aagccgaaaa aaggcaaaac caaggtggaa gggcaacgcg agttgcttct gccgattccc
     7321 ggcaagaagg ccgccgaggc caagccagcc aagacggaag aagcaaagcc cgctcgcgcc
     7381 gcgtcttcgc cgcgacgcaa gaccggatag cgcgacgcgc ctttcagcgg cgtcgaggaa
     7441 aaagactggc gcttcgcccc tcgcccgaca atcatccgca agaagagccg gcgcttggcg
     7501 cggcaagaaa aatcccctcg tcggcaacac cggcgagggg aatcgcaact ggatagcccg
     7561 caacgggccg gcggcgggct ttccggcgcc ggtcagtctt cgacgatccc gacgacttcg
     7621 tgcgtctccg gatcgacgat cacgacatga tcgctgcggt cgatgaaata gtcgtagctg
     7681 cgcacttccg ggatctccgc cacaaccgga ccgggcagcg gctcgaggac gaccgtgtcg
     7741 ggaacgcgag catcgatccg ctcgtcgaac tcgaccgtct tttcggggcg ggcgtggata
     7801 tgccggcgga tcacctggtc ctgctcgcgg gtgaggcgga gcctttcgcg atgggcggcc
     7861 gcggccgatc gggtcgagcc gccgatcact tcgacaacct cgcgggtgtg cggatcgacg
     7921 atgacgatct cgtcttccac catcaccacg tcatacccgc ggaactccgg atattcggcg
     7981 ataatatcgg tcgggatcgg ctcgagccgg acggattccg gcaccgccac gccgacattc
     8041 acgttgaagt tgacggagct cctcgtcacc tccccgtgct tctcgagacg gctgcggaag
     8101 tccgaggcct gctgcgcgtt catcttcatc tttccgtgcg ccatcgcctc gttttgaccc
     8161 gtcgcgccgg cgtttcccgg ctccgccgcg gcgcgccctt cggtcgcgcg cttttgcgac
     8221 gtttcgcc
//
LOCUS       DATGJW010000359         4368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1106613, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000359 DATGJW010000000
VERSION     DATGJW010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4368
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106613"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..218
                     /locus_tag="VKU03_10930"
     CDS             <1..218
                     /locus_tag="VKU03_10930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71824.1"
                     /translation="ILASLQKTPIPTKSGSENDSKKRPSSFSKFLFGRFERFQWLGAQ
                     KKMEMRFCPSDRTKAAEGAFLSPRRYE"
     gene            complement(356..1378)
                     /locus_tag="VKU03_10935"
     CDS             complement(356..1378)
                     /locus_tag="VKU03_10935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HLJ71825.1"
                     /translation="MDDFPFANQRPFPTDRLITVFGGSGFIGRHVVRALVKRGWRVRV
                     AVRRPDLAFFLQPIGTVGQISPVQANLRFPETIEAAMTGAQAAVNVVGIGAQSGKQTF
                     DAVHVEGARAIAKAAASRGVPLVHISGIGADAHSSSPYIASKGRGEDATRDEAPDAVI
                     LRPSVVFGPEDKFLNRFGQLARFSPVLPLFGGGATKLQPVYVGDVALAVANALEGQAK
                     AGATYELGGPEQMSLRHAVEFVCRTTGRRRMLIGVPFGLASLLAGATEIASAVSFGLF
                     PKALTTTRDEVALLRSDNVVSEAAVAAGLTLAGLGVAPQGMEAIAPAYLYRYRRTGQY
                     ENSRFA"
     gene            complement(1498..3576)
                     /locus_tag="VKU03_10940"
     CDS             complement(1498..3576)
                     /locus_tag="VKU03_10940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLJ71826.1"
                     /translation="MVGLNGANERRIAGPRLIAIVGPFQGGKTTLLEAILARGGALAR
                     QGSARDGTSLGDSSPEARAHAMSVEPNIAHLDYMGDRMTFVDCPGSVEFVHDMRNVLP
                     VCDAAVVVCEADARKTPALQVALRELEELRIPRILFLNKIDLAGGSVRETLEMLQPAS
                     RTPLLLRQIPIWENGVASGFIDLALERAFVYREHAPSIVIEMPDGQKPDEKEARYSML
                     ERLADYDDALMEELITEIEPPRDRVFDDLAKELRGGHIVPLLMGSAERGHGVTRLLKA
                     LRHEAPGIAQTRERLGVEAQGPALAHVVRTIHTAHGGKLSLARVLRGAFADNATVASS
                     SGAEDRIAGLSRLMGSASAKLSRAEEGDTVAFAKLEHIATGETFTDAKKAPARVIEIP
                     PPSPTQVIALHVKDRKDDVRLAAALAKLAEEDPALIYRQDQEAGELKLYGQGEMHLRV
                     TIERLAARFGVAVDSAKPSIAYRETIRDAVSARGRHKKQSGGHGQFGDVAIEVKPRQR
                     GEGFAFEERVHGGAVPRQYFSSVEAGSRDALTRGPLGFPVVDVAVTLTDGSYHTVDSS
                     DMAFRAAAKIALGEALPKARPVLLEPILAVSIYVPSEAMARASALVSARRGQILGFEP
                     RPGWDGWDVLRAQIPEAEIGDLIVELRSATAGVGAFETKFDHLAELSGRPAELVIAAR
                     QRTGAGATTG"
     gene            3835..>4368
                     /locus_tag="VKU03_10945"
     CDS             3835..>4368
                     /locus_tag="VKU03_10945"
                     /EC_number="2.7.1.71"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_028175815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate kinase"
                     /protein_id="HLJ71827.1"
                     /translation="MTHLAPPDRDRLMAALSGRSIVLVGMMGAGKTSVGRRLAAKLGL
                     PFVDADAEIETGAQMTVPEIFARFGEAYFRDGERKVIARLMADGQKVLATGGGAFMNE
                     ATRQRIGERGVSVWLKPDFETLMRRVRKRTNRPLLQTADPEETVRKLLAERSPIYSLA
                     DLTIESRDGPHEIVVDAI"
BASE COUNT          767 a         1447 c         1452 g          702 t
ORIGIN      
        1 aaatccttgc gtcgctccaa aagacgccga tcccgacaaa atccggctcg gaaaacgaca
       61 gcaaaaagcg gccatcaagt ttttccaaat ttttatttgg ccgttttgag cgattccaat
      121 ggcttggagc gcaaaaaaaa atggaaatgc gtttttgccc ttccgatcgt accaaggccg
      181 ctgagggcgc tttcctctct ccgcgacggt acgaataata gccgaggcaa agcggtttga
      241 gaagaaattg ttcgcttctt ttcttaagcc gatgatccga aacgcctaaa gcgcaacgac
      301 ctccctccca cgactgcggc gaaagaggcg gcgcacggag cgtagggagg cgagctcacg
      361 cgaaccggct gttctcgtac tgcccggtgc ggcgatagcg gtagagatag gccggcgcga
      421 tcgcctccat gccctgcggc gcgacgccga gtccggcgag cgtcagcccc gccgcgaccg
      481 ccgcctcgga gacgacattg tcgctgcgca acagtgcgac ttcgtcgcgc gtcgtcgtca
      541 acgccttggg gaaaaggccg aacgagacgg cgctggcgat ctcggttgcg ccggcgagga
      601 gcgaagcgag cccaaacggc acgccgatca gcatgcgccg ccgcccggtt gtcctgcaga
      661 cgaattcgac cgcgtggcgc aagctcatct gctccggtcc gccaagctcg taggtcgcgc
      721 cggctttggc ctgcccttcg agcgcgttgg cgaccgccag cgcgacatcg ccgacataga
      781 ccggctgcag cttggtcgcg ccgccgccga acagcggcaa gaccggcgag aagcgggcga
      841 gctggccgaa gcggttgaga aacttgtcct ccggcccgaa cacgaccgaa ggccggagga
      901 tcacagcgtc cggcgcctca tcgcgcgtcg cgtcctcgcc gcggcccttg ctggcgatat
      961 agggcgagga ggaatgcgcg tccgcgccga tgccggatat atggacgagc gggacgccgc
     1021 gcgaggcggc cgccttggcg atggcgcgcg cgccctcgac atggaccgcg tcaaaggtct
     1081 gcttcccgct ctgggcgccg atgccgacga cattgaccgc cgcctgcgcg ccggtcatcg
     1141 cggcttcgat cgtttccgga aaacgaagat tggcctggac cgggctaatc tggccaaccg
     1201 tcccgatcgg ctggaggaag aaggcgagat cgggccggcg caccgccacc cgcacccgcc
     1261 agccgcgctt gacgagagcg cgcaccacat gacggccgat aaagccggag ccgccgaaga
     1321 cggtgatcaa ccgatcggtc ggaaaggggc gctgattggc gaaaggaaaa tcgtccatga
     1381 gggaatgctc cgcgggcgcg gccgaaatgg attcggctcg ttgcataggc gaagcgcgcg
     1441 cttctgtata gtcgcgccag cctgacccga cggcctcgtc ccggcgcgct taatcgccta
     1501 tccagtcgtt gcgcccgcgc ccgtcctttg gcgcgcggcg atgaccagtt cggccggtcg
     1561 gccggaaagt tcggcgagat gatcgaattt ggtctcgaac gcgccgacgc cggcggtggc
     1621 cgagcgcaat tcgacgatca gatcgccgat ttcggcctcc gggatttgcg cccggagcac
     1681 gtcccagccg tcccagcccg gccgcggctc gaagccgaga atctggccgc gccgcgccga
     1741 aaccaaggcg ctcgcccgcg ccatcgcctc cgaaggcaca tagatcgaga cggcgaggat
     1801 cggctcgagc aaaaccggcc gggctttcgg cagggcctcg ccgagcgcta ttttcgcggc
     1861 ggcgcgaaac gccatgtcgg acgaatcgac ggtgtgatag gagccgtcgg tcaaagtcac
     1921 cgcgacatcg acgaccggga agccaagcgg cccgcgcgtc agcgcgtcgc gcgagccggc
     1981 ttcgaccgag gaaaagtatt gcctcggcac ggcgccgcca tggacgcgct cctcgaaggc
     2041 gaaaccctcg ccgcgctgcc gcggcttcac ctcgatcgcc acgtcgccga attggccgtg
     2101 cccgccggat tgcttcttgt ggcggccgcg cgccgagacc gcgtcgcgaa tcgtctcgcg
     2161 ataggcgatg gacggcttgg cgctgtcgac ggcgaccccg aagcgcgccg ccaagcgctc
     2221 gatcgtcacc ctcaaatgca tctcgccctg gccatagagc ttgagctcgc cggcttcctg
     2281 atcctgtcga tagatgaggg ccgggtcttc ctcggcaagc ttggcgagcg cggcggcgag
     2341 gcgcacgtcg tccttgcggt ccttgacatg cagggcgatc acctgggtcg gcgagggcgg
     2401 cggaatctcg atgacgcgcg ccggcgcttt cttggcgtcg gtgaaggtct cgcccgtcgc
     2461 gatatgttcg agcttggcga aagcgaccgt gtcgccctct tcggcgcgcg acaatttcgc
     2521 gctggccgag cccatcaggc gggaaaggcc ggcgatccgg tcctccgcgc cgctcgatga
     2581 ggcgacggtc gcgttatcgg cgaaggcgcc gcgcaggacg cgggcgagcg acagcttgcc
     2641 gccatgggcg gtatggatcg tgcgcacgac atgggcgagg gccggcccct gcgcctcgac
     2701 gccgaggcgt tcgcgcgtct gagcgattcc cggcgcttcg tggcgcaacg ccttcaagag
     2761 ccgggtgacg ccatggccgc gctccgccga gcccatcagc agcggcacga tatggccgcc
     2821 gcgcaattct ttggcgagat cgtcgaagac gcgatcgcgc ggcggctcga tctcagtgat
     2881 cagctcctcc atcagcgcgt cgtcgtaatc ggcgagacgc tcgagcatcg aatagcgcgc
     2941 ctccttctcg tccggcttct ggccatcggg catttcgatg acgatcgacg gggcatgttc
     3001 gcggtagacg aaggcgcgct cgagagcgag gtcgatgaag ccggaagcga cgccgttctc
     3061 ccaaatcggg atctggcgca gcagcaaagg cgtgcgcgac gccggctgca gcatttccag
     3121 cgtctcgcgc acgctgccgc cagcaaggtc gatcttgttg agaaacagga tgcgcggaat
     3181 gcgcaattct tcgagctcgc gcagcgccac ctgcagagcc ggcgttttgc gggcgtcggc
     3241 ctcgcagacg accaccgcgg cgtcgcagac cggcaggaca ttgcgcatat cgtggacaaa
     3301 ttcgaccgag ccggggcaat cgacgaaggt catccggtcg cccatgtaat cgagatgagc
     3361 aatgttgggc tcgacgctca tcgcatgggc tcgggcttcc ggactggaat cgccgaggct
     3421 ggttccgtcg cgcgccgagc cctgccgcgc cagagcgccg ccgcgcgcca atatcgcctc
     3481 caggagagtc gttttgccgc cttgaaaggg gccgacgatg gcgatgagcc gcggccccgc
     3541 aatccgtctt tcgttcgcgc cgttcaggcc gaccatgacg tcctcctcga tctttttggc
     3601 gaggaccttc cgctcggcgg cgggaggccc gcgcaagtcc ccgctagcgt cgcaaaagct
     3661 ttaggagagc gcaaggcgtg acgaaggcgc aggagagaat ccgcggcggc gcagcgcccc
     3721 cgccgcgatc gcctctcttt cgcaattccc tgataacatt gtatggactt ggcgaaaccg
     3781 ggcgggcggc cgtcgcaacg ggacgggcgg cgccttcgca ttttggattg gagcatgacg
     3841 catttggcgc cgcctgatcg cgaccgcctc atggccgccc tgagcgggcg ctcgatcgtg
     3901 ctggtcggca tgatgggcgc cggcaagacg tcggtcggtc gccggctcgc cgccaagctc
     3961 ggcctgccct tcgtcgacgc cgacgccgaa atcgaaaccg gcgcgcagat gaccgtgccg
     4021 gagattttcg cgcgcttcgg cgaagcctat ttccgcgacg gcgaacgcaa agtgatcgcc
     4081 cggctaatgg ctgacggcca gaaagtgctc gccaccggcg gcggcgcctt catgaacgag
     4141 gcgacccgtc agcggattgg cgagcgcggc gtctcggtct ggctgaagcc cgatttcgaa
     4201 acgctgatgc gccgcgtccg caaacggacc aatcggcccc tgctgcagac cgccgatccg
     4261 gaagaaaccg tgcgcaagct gctcgccgag cgctcgccga tctacagcct cgccgacctg
     4321 acgatcgaat cgcgcgatgg tccgcacgag attgtggtcg acgcgatt
//
LOCUS       DATGJW010000360         3042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_586684, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000360 DATGJW010000000
VERSION     DATGJW010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3042
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_586684"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            327..998
                     /locus_tag="VKU03_10950"
     CDS             327..998
                     /locus_tag="VKU03_10950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71828.1"
                     /translation="MADDLTSEFRRPVPLALAAVAVIGWLLVAYFASQVSEVQSQMHD
                     ALGRAEKARESMAADLQNLQKAAGNLADVEKQLNDAKNALAESTAARAAAQNDLAALN
                     KQIAEARLAASGASDEAKAKAGDLQAVEDKLKAANGQLAAVKPQIDNATAERDEVQKA
                     VDQAKAALADLQRQSDSLSKEVAEAKSQLADLKQQGDAANRALAEAQTKLQATQKQME
                     DQQKP"
     gene            complement(1005..2099)
                     /gene="mtnA"
                     /locus_tag="VKU03_10955"
     CDS             complement(1005..2099)
                     /gene="mtnA"
                     /locus_tag="VKU03_10955"
                     /EC_number="5.3.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046523 -
                     S-methyl-5-thioribose-1-phosphate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose-1-phosphate isomerase"
                     /protein_id="HLJ71829.1"
                     /translation="MKIDGKPYRTIWPSADGQAVEVIDQTVLPHRFAIRRIAGLEDAF
                     DAIRTMVVRGAPLIGATAAYGLALALRRDPSDAGLEASHRRLAGARPTAVNLRHALDD
                     VRAHVARLPSAERARAAFARAAAICEEDVENCRLIGEAGLPLIEAIHRAKNRPVNILT
                     HCNAGWLATVDWGTATAPIYMAHDRGVPVHVFVDETRPRNQGAALTAFELGQHGVPHQ
                     IVVDNAGGHLMQHGKVDLVIVGADRVTARGDAANKIGTYLKALAARANQVPFYVAVPQ
                     TTIDWTIEDGVRDIEIEERDSAEVTHIVGRLENGDIVRVAIGAPGSPALNPAFDVTPA
                     ELITGLITDRGVSPATRDGLVGLFPERKAA"
     gene            2287..>3042
                     /locus_tag="VKU03_10960"
     CDS             2287..>3042
                     /locus_tag="VKU03_10960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008184 - glycogen phosphorylase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen/starch/alpha-glucan phosphorylase"
                     /protein_id="HLJ71830.1"
                     /translation="MDQVDLQAPPIAEQRPRHPDADAFSAEALREAVLAKLTYSVGKD
                     PVAASQRDWFLAVAFATRDIIVDRWMNSTRETYQDDRKRVYYLSLEFLIGRMLYDSMT
                     NLGIVEPMRAALSQLGVDLVVLRRIEPDAALGNGGLGRLAACFMDSMATLSIAAHGYG
                     IRYDNGLFRQVIRDGWQQEIPEDWLAFGNPWEFARPESNYAIGFGGQVEAVPGLGDKM
                     RHVWKPGETVEAVGYDTPIVGWRGRHVNTLRLWS"
BASE COUNT          542 a         1026 c          991 g          483 t
ORIGIN      
        1 aggattgctt cggctcgggc gagcgccatt gttccggcca atggcgatgg ccgcgcgccg
       61 cctggaccgc cagattgagg aaggaaaatt tcaccggccg gcgctctccg aggcgcgaaa
      121 atcgttgcgc ctggctctat acgccggccg gcgcgtttgc gccccccata attgttcaat
      181 acccgcccaa agtcttgtca tggcgtcaat tcgtcctgga ttcggcgccg gggagcatgc
      241 caatattttt aggcctgccg ggcttggcga ggatcggccg cggacgccgt tctcgtcgcc
      301 aaaaacggcg attgggggag gcgcgcgtgg ccgacgatct gacgagcgaa ttccgccggc
      361 cggtgcccct ggccttggcc gcggtcgccg tgatcggctg gctgctggtc gcctatttcg
      421 cctcgcaggt ctccgaggtc cagagtcaaa tgcatgacgc gctcgggcgg gccgagaagg
      481 cgcgcgagag catggccgcc gacctgcaaa atctgcaaaa ggccgccggc aaccttgccg
      541 acgtcgaaaa gcagctcaac gacgccaaga acgcgctcgc cgaatcgacc gccgcccgcg
      601 ccgcggcgca gaacgatctc gccgcgctca acaagcagat cgccgaggcg cgcctcgccg
      661 cctccggcgc gagcgacgag gccaaggcaa aggccggcga cctgcaagcg gtcgaggata
      721 agctgaaagc cgccaacggc cagctcgccg cggtcaagcc gcagatcgac aatgcgacgg
      781 ccgagcgcga cgaggtccag aaagccgtcg atcaggccaa ggcggcgctt gccgatctcc
      841 agcggcaaag cgattcgctg tccaaagagg tggccgaggc gaaaagccag ctcgccgacc
      901 tcaagcagca gggcgacgcg gcgaaccgcg cgctcgccga ggcgcagacc aagctgcaag
      961 cgactcagaa gcagatggag gatcagcaga agccctaaag gcgctcacgc cgcctttcgt
     1021 tccgggaaaa gtccgaccaa cccgtcgcgg gtcgctggac tgacgccgcg gtcagtgatc
     1081 agcccggtga tcagctcggc aggcgtcacg tcgaaagccg gattgagcgc cggactgccc
     1141 ggcgcgccga tcgccacccg gacgatgtcg ccgttttcga gccggccgac aatatgggtg
     1201 acctccgcag aatcacgctc ttcgatctcg atgtcgcgaa cgccgtcctc gatcgtccaa
     1261 tcgatcgtgg tctgcggaac cgcgacatag aaggggacct gattggcccg agcagcgagc
     1321 gccttcagat aggtcccgat cttgttggcg gcgtcgccgc gcgcggtgac cctatcggcc
     1381 ccgacgatga cgagatcgac cttgccatgc tgcatgagat ggccgccggc attgtcgacg
     1441 acgatctggt gcgggacgcc gtgctggccg agttcgaaag cggtgagcgc cgccccctga
     1501 ttgcgcggcc gggtctcgtc gacaaagaca tggaccggaa cgccgcgatc atgcgccatg
     1561 taaatcggcg ccgtcgccgt gccccagtcg accgtcgcca gccagccggc gttgcaatgg
     1621 gtgagaatat tcaccgggcg attcttggcg cggtgaatcg cctcgatcag cggcaggccg
     1681 gcctcgccga tcagccggca attttcaacg tcctcttcgc aaatcgccgc ggcgcgggcg
     1741 aacgcggcgc gggcgcgctc agcggaaggc agacgcgcga catgcgcccg cacatcgtca
     1801 agggcgtgcc tgagattgac cgcggttgga cgcgcgccgg cgagccggcg atgcgacgct
     1861 tcaagcccag cgtcggacgg atcgcgcctc agcgccagcg ccaacccata agcagccgtc
     1921 gcgccgatca gcggcgcgcc ccgcacgacc atcgtgcgga tcgcatcgaa cgcgtcttcg
     1981 aggccggcga tccgcctgat cgcaaaacgg tgcggcagca cggtctggtc gatgacctcg
     2041 accgcctggc cgtcagcgct gggccagatg gtgcggtaag gcttgccgtc aattttcatc
     2101 gttccgctcc aagcctcaaa acgccctcgt cgcctaccgg attatcgcgc cgacggccga
     2161 gtttggccag cgcgagcggc cgccgtcgcc tcctttcgcc cgctttcgcc caaactggac
     2221 aggacgcccg aaagaggctt ttctcgcagg cgagccttga cgccgcacag aacggacatc
     2281 ctgcgcatgg accaagtcga cctccaggcc cctcccatcg ccgagcaaag gccgcgtcat
     2341 ccggacgccg acgccttttc ggcggaggcg ctgcgcgagg ccgtgctcgc caagctcacc
     2401 tactcggtcg gcaaggaccc ggtcgccgcc agccagcgcg actggtttct cgccgtggcc
     2461 tttgcgacgc gcgacatcat cgtcgaccgc tggatgaatt cgacgcgcga gacctaccag
     2521 gacgaccgca agcgcgtcta ttacctgtcg ctggaatttc tcatcggccg gatgctttac
     2581 gattcgatga caaatctcgg cattgtcgag ccgatgcgcg cggccttgtc gcagctcgga
     2641 gtcgaccttg tcgtcctgcg ccggatcgag cccgacgccg cgctgggcaa tggcggcctt
     2701 ggccgcctcg ccgcctgctt catggattcg atggcgacgc tctcgatcgc cgcccatggc
     2761 tatggcatcc gctacgataa cggtcttttt cggcaagtca tccgcgacgg gtggcagcag
     2821 gaaattcccg aggactggct cgccttcggc aatccgtggg aattcgcgcg gccggagagc
     2881 aattatgcga tcggctttgg cggccaggtc gaggcggtgc cgggactcgg cgacaagatg
     2941 cgccatgtct ggaagccggg cgagacggtc gaggcggtcg gctatgacac gccgatcgtc
     3001 ggctggcgcg gccgccatgt gaacacgcta cgcctctggt cg
//
LOCUS       DATGJW010000361         2948 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_69188, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000361 DATGJW010000000
VERSION     DATGJW010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2948)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2948)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2948
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69188"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..211)
                     /locus_tag="VKU03_10965"
     CDS             complement(<1..211)
                     /locus_tag="VKU03_10965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71831.1"
                     /translation="MTSVSLPWRRPALPRRLVARLTGAAFAACSALALAGCGPSGGMN
                     LGSNDAAAAVPTTPVQSQPLNGPTVG"
     gene            449..1363
                     /gene="rsmI"
                     /locus_tag="VKU03_10970"
     CDS             449..1363
                     /gene="rsmI"
                     /locus_tag="VKU03_10970"
                     /EC_number="2.1.1.198"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019905434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0000451 - rRNA 2'-O-methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (cytidine(1402)-2'-O)-methyltransferase"
                     /protein_id="HLJ71832.1"
                     /translation="MARENAEEIRGPSAFTALGLRAEADPIAPGLHVVATPIGNLRDI
                     TLRALSTLAAADAVVAEDTRVTRTLLAHYGIATPLVAYHEHNAAVVRPHLLARLAGGA
                     ALALVSDAGTPLVSDPGYRLVAEALEASIPVTSVPGPSAVLAALVVAGLPTDRFYFEG
                     FLPPKSAARRARIVELAAIPSTLVFFESPRRLADTLADLAVELGPRSAVVARELTKLY
                     ETTRRGPLDRLAAEFAGEAPPKGEIVVLVGPPEGAPPVAEEEIDKRLAAALESLSVKD
                     AAAAVSAETGAPRRKVYARALALSGAKR"
     gene            1365..1760
                     /locus_tag="VKU03_10975"
     CDS             1365..1760
                     /locus_tag="VKU03_10975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612006.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YraN family protein"
                     /protein_id="HLJ71833.1"
                     /translation="MAAPSARKERSAAKWRAHRLGLRAEWLAAAFLVVKGYRILARKF
                     VVAGGEIDLVARRGDVIAFVEVKARPGFDEALLAIDSVKRRRIARAARLWLTKNPGAM
                     QATLRGDAVLIAPLRWPRHVEAAFELELE"
     gene            1899..2846
                     /gene="gshB"
                     /locus_tag="VKU03_10980"
     CDS             1899..2846
                     /gene="gshB"
                     /locus_tag="VKU03_10980"
                     /EC_number="6.3.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386035.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004363 - glutathione synthase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006750 - glutathione biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione synthase"
                     /protein_id="HLJ71834.1"
                     /translation="MPLAVAVQMDPIEKIRIAGDSTFALLLEAQKRGHKLLYYTPDCL
                     TMRGPRVLAQAQPLEVKDLQGEHAKLGPATTVDLADLDVVLLRQDPPFDLAYITTTHF
                     LDRIHPKTLVVNDPASVRNAPEKLFVMDFPQLMPPTLISRDRDAIEAFRAEHGEVVMK
                     PLHGFGGVSVFKVGPKDANFGSLYDLFATTFREPWVTQKFLPAIAKGDKRIVLVEGEA
                     LGAVNRVPSADDIRANMARGGAAAATDLTPREREICATIGPELKRRGLIFVGIDVIGD
                     YLTEINVTSPTGIRAIERIGGPNLAVAIWDAIERKRSKN"
BASE COUNT          478 a         1021 c          952 g          497 t
ORIGIN      
        1 cgccgacggt cggcccgttg agcggctgcg attgcaccgg cgtcgtcgga acggccgccg
       61 ccgcatcgtt cgagcccaaa ttcatgccgc cgctggggcc gcagccggcg agcgccaagg
      121 ctgagcaggc ggcgaaagcg gcgccggtca ggcgcgcgac gaggcgccgc ggcagcgccg
      181 gccgtcgcca aggcagcgag acactcgtca agaagacccc tcctgagccg gcgattgtcg
      241 atatgtcgcg ccgatggcgc ggcgagcgcc gactctcata cgcgcgctca aggcggcgct
      301 cagcgctcat cgcctttaaa tgtggcattt taagtcttga tgggtaaacc gttccttgct
      361 ggctgataac cgagcttgtc cgcccggcct atggttctgt ataaggaaca ttcggacgaa
      421 ataacgaacg gcgcggagcg aggcgggcat ggcgcgagaa aatgccgaag aaatcagagg
      481 gccgagcgcc ttcaccgccc tcggcttgcg cgccgaggcc gacccgatcg cgcccggcct
      541 gcatgtcgtg gcgacgccga tcggcaattt gcgcgacatc accttgcgcg ctttatcgac
      601 cctcgccgcc gccgacgcgg tggtcgccga agacacccgc gtcacccgca ccctgctcgc
      661 ccattacggc atcgccacgc cgctcgtcgc ctatcacgag cacaacgccg cggtggtgcg
      721 gcctcatttg ctggcgcggc tcgccggcgg cgcggcgctg gctctcgtct ccgacgcagg
      781 cacgccgctc gtctccgatc ccggctaccg gctggtcgcc gaggcgctgg aggccagcat
      841 cccggtcacg tccgtgccgg ggccgtcggc cgtgctggcc gctctggtcg tcgccggcct
      901 gccgaccgac cgcttctatt ttgaaggctt cctgccgccg aaaagcgccg cgcggcgcgc
      961 ccgcatcgtc gagctcgccg cgattccctc gaccttggtc tttttcgaat cgccgcgccg
     1021 cctcgccgat acgctcgccg acctggccgt cgaattgggg ccgcgctccg ccgtcgtcgc
     1081 gcgcgaactg accaagctct acgagacgac gcggcgcggg ccactcgacc gcctggcggc
     1141 ggaatttgcc ggcgaggcgc cgcccaaggg cgagatcgtc gtgctggtcg ggccgcccga
     1201 gggagcgccg ccggtcgcgg aggaggaaat cgacaagcgc ctcgccgccg cgctcgaatc
     1261 gctgagcgtc aaggacgcgg ccgcggcggt ttcggccgaa accggcgcgc cgcgccggaa
     1321 agtctacgcc cgtgcgctgg cgctttccgg cgcgaagcgc tgatatggcc gcgccgagcg
     1381 cccgaaaaga acgatcggcg gcaaaatggc gcgcgcaccg cctcggcctg cgcgccgaat
     1441 ggctcgcggc ggcttttctt gtcgtcaagg gctatcgcat cctggcgcgc aaattcgtcg
     1501 tcgccggcgg cgagatcgat cttgtcgcgc ggcgcggcga cgtcatcgcc tttgtcgaag
     1561 tcaaggcgcg gcccggcttc gacgaagcgc tgctcgcgat cgattcggtc aaaaggcgcc
     1621 gcatcgcccg cgccgcccgg ctgtggctga cgaaaaatcc cggcgccatg caggcgacgc
     1681 tgcgcggcga cgccgtgctg atcgcgccgc tgcgctggcc gcggcatgtc gaggcggcgt
     1741 tcgagctgga attggagtag gggcgcggga cagctctgtc tttcgctgat gcgagaggcc
     1801 gacgctgaca tcgggggaaa gtttgcttaa agaaaggcga tgtcggcttt cgatgttttg
     1861 cgcaagtcct tcatgaccgt tttagccagg aacttcccat gcccctcgcc gtcgccgtgc
     1921 agatggaccc gatcgagaag atcagaatcg ccggcgattc caccttcgcc ctcctgctcg
     1981 aagcgcagaa gcgcggccac aagctcctct actatacgcc cgactgtttg accatgcgcg
     2041 gtccgcgcgt gctcgcgcag gcgcagccgc ttgaagtcaa ggacctgcaa ggcgagcacg
     2101 ccaagctcgg ccccgccacg accgtcgatc tcgccgacct cgacgtcgta cttttacggc
     2161 aggatccgcc cttcgatctc gcctatatca cgacgacgca tttcctcgac cgcatccatc
     2221 cgaagaccct cgtcgtcaac gatccggcct cggtccgcaa tgcgccggaa aagctcttcg
     2281 tcatggactt cccgcaattg atgccgccga ccctgatctc gcgcgaccgc gacgcgatcg
     2341 aagcgttccg cgccgagcat ggcgaggtgg tgatgaagcc gctgcacggt tttggcgggg
     2401 tgagcgtgtt caaggtcggg ccgaaagacg ccaatttcgg ctcgctctac gacctctttg
     2461 cgacgacatt ccgcgagcct tgggtgacgc agaagttcct tcccgctatc gccaagggcg
     2521 acaagcgcat cgtcctggtc gagggcgagg ctttgggcgc cgtcaatcgc gtgccttcgg
     2581 ccgacgacat ccgcgccaac atggcgcgcg gcggcgccgc cgcggcgacc gacctgaccc
     2641 cgcgcgagcg cgaaatttgc gcgacgatcg gcccagaact gaagcgccgc ggcctgatct
     2701 tcgtcggcat cgatgtcatc ggcgactatt tgaccgagat caacgtcacc tcgccgaccg
     2761 gcatccgcgc catcgagcgc atcggcggcc ccaatctcgc cgtcgcgatc tgggacgcaa
     2821 tcgagcgcaa gaggagtaaa aattaggcga tatcttcggc ttgtccgggt tcgaggtatc
     2881 tgaaagggcc gattgcccct ttcttgcgag gtggcgcacg ttttcccacg tacttgtccc
     2941 aaacgtca
//
LOCUS       DATGJW010000362         9756 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1106794, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000362 DATGJW010000000
VERSION     DATGJW010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9756)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9756)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9756
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1106794"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1331
                     /locus_tag="VKU03_10985"
                     /pseudo
     CDS             172..1331
                     /locus_tag="VKU03_10985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083709.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
     gene            1416..2180
                     /locus_tag="VKU03_10990"
     CDS             1416..2180
                     /locus_tag="VKU03_10990"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71835.1"
                     /translation="MTFSLETKALAIRFGGHVAVDAVSCAFRPGELTAIVGPNGAGKT
                     TYFNLISGQLRATSGAVLLNGADITSLGAAQRASRGLGRAFQLTNLFPGLSVAENIRL
                     AVQAAEGVHYEMMRPWRARGDLIARADEILEEVALTGRRNAAAAALSHGDQRKLEVAL
                     MIALKPSVFMFDEPTAGMSVDEVPVVLDLIARLKQDASKTILLVEHKMDVVRSLADRI
                     IVLHNGRLMADGEPAEIIASPIVQEAYLGVARARPA"
     gene            2177..2899
                     /locus_tag="VKU03_10995"
     CDS             2177..2899
                     /locus_tag="VKU03_10995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971103.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71836.1"
                     /translation="MSALLTLSGVHTHIGRYHILQGVDLDAPEGKTTMLLGRNGAGKT
                     TTLRTIMGLWRASAGTVTFAGQALAPLATPAIARAGISYVPETMAVFSDLTVRENFIL
                     AARDGPLDDARLQWIFGFFPALKKFWLSRAGSLSGGQKQMLSIARAIVEPRRLLLIDE
                     PTKGLAPAIVGGLIECLREIKRAGVAILLVEQNFRVAQEIGDEVRVMDNGRIVHEGPM
                     AALAADGALQNRLLGLSLDAHQ"
     gene            2896..3918
                     /locus_tag="VKU03_11000"
     CDS             2896..3918
                     /locus_tag="VKU03_11000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ71837.1"
                     /translation="MSAIEKSGDIGAALPRRKIDFAPALLPFLIALVAAPLIGSTSTF
                     FTLTFASLAMGLMLFTMASGFTLVFGLMDVLNFGHGAFISIGAYMATLVFAPLILWSQ
                     AGSLGLNLGVIFLAAAVAAIVCGALGLVFERLLVRPVYGQHLKQILVTTGGLIVVEQL
                     LYALFGPDTIPLALPTALRGAFVIGDAAIEKFRLLAVIVGLVIFAGLSLTLNYTKIGL
                     LIRAGVENREMVEALGYRINRLFLAVFMAGSALAGLGGVLWALYREQVHASVGESLTV
                     LIFIVVITGGLGSIGGCFLGATLVALVANYAGFLAPKIALVSNIALMVAVLLWRPRGL
                     YPAGGR"
     gene            3915..4985
                     /locus_tag="VKU03_11005"
     CDS             3915..4985
                     /locus_tag="VKU03_11005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008135542.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ71838.1"
                     /translation="MNLLSGDPPRGRALAALLALIVVILALAPFLFPGAKSLNVAATI
                     LIYALLVASYDLLLGYTGIVSFAHVMFYGVGAYGVAIALYGLGPGWGALLIGVAAALP
                     VSAALALAIGLFSLRVQTIFFAMVTLAAASAFNVLASQLDWLTGGEDGRSFQVPAALR
                     PGFHLVSGRVFGVEINGRMISYYLVFAACALLFLALLRVVNSPFGRVLQAIRENPFRA
                     EALGYRIVYHRTLATCLSAIVASLAGALNAIWQHYVGPDTALNLSIQIDVLVMVVIGG
                     MGSLYGAIAGAALFVIARNYLQGLLGALSAAAAGAGLTLIAGLLHPDRWLMWLGLMFI
                     LAVYFVPTGIVGRLRAEDKGSG"
     gene            complement(4977..5375)
                     /locus_tag="VKU03_11010"
     CDS             complement(4977..5375)
                     /locus_tag="VKU03_11010"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ71839.1"
                     /translation="MAQPKVPTQIIPYIFYRDVPAALDWLSRAFGFKEIMRVGTPSGG
                     MHGEMELDGQKIMMGQGGKQWGMINAREAGVATQGVFVYLADVDAHYERARAAGAEIE
                     KPPENVDYGRTYTARDPDGHPWFFATPPSA"
     gene            complement(5503..6312)
                     /locus_tag="VKU03_11015"
     CDS             complement(5503..6312)
                     /locus_tag="VKU03_11015"
                     /inference="COORDINATES: protein motif:HMM:NF024242.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLJ71840.1"
                     /translation="MAFLEPDVRFAPYVARLNVYRERDTAFTRRAEPPSGLATLVFNL
                     GAELRVEHPVKTFNAYGAGGAFYTGISSTFAITETDRAQEGAQAMLTPLGARLLVDFP
                     LAEIGDRLIDPADLFGAAARATIGRLMACNSEEARLAILEAAMAERLERARRPPPADL
                     AFAAQRLQASGGRLSVAALAEEIGCGRKHLTMRFAREFGIAPKLLGRVLRFDRALRRA
                     RSGAVPSLAALADSCGYADQAHLTRDFKEFAGAPPAAFMRRALPDDGGFPL"
     gene            complement(6436..7689)
                     /locus_tag="VKU03_11020"
     CDS             complement(6436..7689)
                     /locus_tag="VKU03_11020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010912569.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HLJ71841.1"
                     /translation="MSLSGVSDEHEAHAAAAAAARRSYGRLVALLSARTRDVAGAEDA
                     LSEAFAAALADWPKSGVPRSPEAWLLAAARRRSIDAARRRRTAAEAGGHVQLLAEEMA
                     AMAEETAPIPDERLSLMFACAHPAIEASIRAPLILQTVLGFDAASVASAFLVAPATMG
                     QRLARAKAKIKLAGIPFRTPEREDMAERLDAVLAAIYAAFAEGWSDPAGAEAKRRNLA
                     EEAIWLGRLIAELLPNEAEALGLLALMLYSDARRGARRDQEGEFVPLAEQDPACWDER
                     MIAEAESLLSRAHGYGAIGRYQLEAALQSAHVARRLGRGVDWPAIVTLYDALCRVTGS
                     PVAAINRAVALAEAQGAAAGLAALAAMAGDERLAQYQPYWAARAELLARTGERAEAQA
                     AYDQAIGLEADPAVRRFLQRRRAAV"
     gene            complement(7686..8033)
                     /locus_tag="VKU03_11025"
     CDS             complement(7686..8033)
                     /locus_tag="VKU03_11025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLJ71842.1"
                     /translation="MQYLLLIHSSEAAFAAIPKDQIEQAMGAYMAYTEALKKAGALVN
                     SNRLQPVAAATTVRMANGKTQVLNGPYAETKEQLGGYYLIEAPDLDAAISWAARCPGA
                     SHGTVEIRPVWTM"
     gene            8303..8728
                     /locus_tag="VKU03_11030"
     CDS             8303..8728
                     /locus_tag="VKU03_11030"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLJ71843.1"
                     /translation="MGASTEGPMNNNVSAVKAAYEAVARGQVEVLFGLLADDIRWRAT
                     GPGPLAGEYIGKAEIGRFFGKMGETYGDTFRLDVVDVLGSADRVIVLTEEKGEYRGQP
                     VAWRSAHVYTFENERCVSFLSFQDDSYINFWSARRSGVE"
     gene            8953..9681
                     /locus_tag="VKU03_11035"
     CDS             8953..9681
                     /locus_tag="VKU03_11035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011156081.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OmpA family protein"
                     /protein_id="HLJ71844.1"
                     /translation="MTDVFLCEGARTPIGRYGGALASLRPDDLLAHAIKAALAKTPKL
                     DPAAIDDVYAGCANQAGEDNRNVARMALLLAGLPQEIPGVTINRLCGSGLDAVGAAAR
                     AIRAGEIDLAIAGGVESMSRAPFVQGKATEAFSRKAEIFDTTIGWRFVNPLMKAQYGV
                     DSMPETAENVAEEFQISRADQDAFALRSQQRAAAAQKYLTDLGVDASRIATISYGKDK
                     PVNTAHTPEAWAENRRDEIQPRSP"
BASE COUNT         1541 a         3261 c         3216 g         1738 t
ORIGIN      
        1 ttgacggtgc ggcgccgctt ctccattgtg cgctgcatcg gagggcgtct tcaaattttc
       61 ggcattgccg gccctgatcg ccgtcagggc ggccggatcg gctgaattcg agcgctttcc
      121 caacctgaac atcgccgggg agggcgcatg cacaatttcg tgaaaagtct catggttgcc
      181 gccggcgcgc tcatcgccgg cgcggcttac gccgacgatc tcaatgtcgc gctgatttat
      241 ggccgcaccg gtccgctcga agcctacgcc aagcagaccg agaccggcct gcgcctcggc
      301 ttcgaatacg cgaccaaggg cacgatgacg gtcgacgggc ggaagatcaa catcatcctc
      361 aaagacgatc agtccaagcc cgacctcgcc aaggcgccct cgccgaggcc tatgaggacg
      421 accatgccga catcgccatc ggcaccacct cctcggccgc ggcgatcgcc atgctgccgg
      481 tcgccgagga gcataaaaaa atcttgatcg tcgagccggc cgtcgccgac cagatcaccg
      541 gcgacaagtg gaatcgctat attttccgta cggcgcgcaa ctcgtcgcag gacgcgatcg
      601 ccaatgcggt ggcgatcggc aagccgggcg tgacgatcgc gactctgggc caggattacg
      661 cgttcggcca tgacggcgtc gcggccttca aggaggcgct cgccaagacc ggcgcgaccc
      721 tggtcgacga ggaatatgcg ccgatcaaga cgacggattt caccgccgtc ggccagcgcc
      781 tgttcgacgc gctgaaggac aagccgggcc gcaagatcat ctgggtgatc tgggccggcg
      841 gcggcgatcc gctcgacaag attcaggata tggatccggg ccgctacggc atcgagcttt
      901 cgaccggcgg caacatcctg ccggcgctcg ccgcctacaa gcgcttcccc ggcatggaag
      961 gcggcgcgta ctactattat gggctgccga agaatccggt caacgattgg ctggtcgccg
     1021 aacacaagaa gcgcttcaac tcgccgccgg atttcttcac ctgcggcggc ttcgccgcgg
     1081 cgatggcggt ggtgacggcg gtcgagaagg cgaaatcgac cgacagcgag aagctgatcg
     1141 cgacgatgga aggcatggat ttccaaacgc ccaaaggcaa gatgatcttc cgcaaggagg
     1201 atcaccaggc gctccagtcc atgtaccatt tcaaggtcaa ggtcgatccg aatctgccct
     1261 gggccgatct ggagctcgtc aacgagatca agatcgagga tatgaacgtg ccgatccgga
     1321 ataagcggta gaagggcaat cggcagtcgg caatcggcaa ttggaacgcc gaggtcgccc
     1381 gactgccgac tgcctaatgc cgactgccga catcgatgac tttctccctc gaaaccaagg
     1441 ccctcgccat ccgcttcggc ggccatgtcg cggtcgacgc ggtttcgtgc gcgttccggc
     1501 ccggcgagct caccgcgatt gtcggcccga acggcgccgg caagacgacc tatttcaatc
     1561 tgatctccgg acaattgcgg gcgacgagcg gcgcggtgct gctgaacggc gccgacatca
     1621 ccagcctcgg cgcggcgcag cgggcgagtc gcggcctcgg ccgcgctttt cagctcacca
     1681 atctctttcc cggcctttcc gtcgccgaga acattcggct cgccgtccaa gccgccgaag
     1741 gcgtgcacta tgagatgatg cggccgtggc gcgcgcgcgg cgatctgatt gcgcgcgccg
     1801 atgaaatcct cgaagaggtg gccctgaccg gtcggcgcaa cgccgcggcg gcggcgctgt
     1861 cgcatggcga tcagcgcaag ctcgaagtcg ccttgatgat cgcgctgaag ccgtcggtct
     1921 tcatgttcga cgagccgacc gccgggatga gcgtcgacga agtcccggtc gtgctcgatc
     1981 tgatcgcccg cctgaaacag gacgcatcga agaccatcct cctcgtcgag cataagatgg
     2041 acgtggtgcg ctcgctcgcc gaccgcatca tcgtccttca caatgggagg ctgatggccg
     2101 acggcgagcc ggccgagatc atcgcctcgc cgatcgtgca ggaagcgtat cttggcgtcg
     2161 cccgggcgcg gccggcatga gcgcgctgct gactttgagc ggcgtgcata cgcatatcgg
     2221 ccgctatcac attttgcagg gcgtcgatct cgacgcgccg gaaggcaaga cgacgatgct
     2281 gctcggccgc aacggcgccg gcaagacgac gactttgcgt acgatcatgg gcctgtggcg
     2341 cgcctcggcg ggtacggtga cctttgccgg ccaagccctc gcgccgcttg cgacgccggc
     2401 catcgcccgc gccggcatct cctatgtgcc ggaaacgatg gccgtgtttt cggacctgac
     2461 ggtgagggaa aacttcattc tcgcggcgcg cgacggcccg ctggacgatg cgcgcctgca
     2521 atggattttc ggcttctttc ccgccttgaa gaaattctgg ctgtcccgcg ccggctcgct
     2581 atcgggcggc cagaaacaga tgctgtcgat cgcgcgcgcc attgtcgagc cgcgccggct
     2641 tttactgatc gacgagccga ccaaaggcct cgcgccggcg attgtcggcg gtctgatcga
     2701 gtgcctgcga gagatcaagc gcgccggcgt cgccattctg ctggttgagc agaatttccg
     2761 cgtcgcccag gagatcggcg acgaggtgcg ggtgatggac aatggccgga tcgtccatga
     2821 aggccccatg gcggctctcg ccgccgacgg cgcgctgcag aatcgcttgc ttggcctgtc
     2881 gctggacgcc catcaatgag cgcaatcgaa aagagcggcg acatcggcgc cgcgctgccg
     2941 cggcggaaaa tcgactttgc gccggcgttg ctgccgttcc tgatcgcgct tgtcgccgcg
     3001 ccgctgatcg gctcgacctc gaccttcttc acattgacct tcgccagcct cgccatgggc
     3061 ctgatgctgt tcaccatggc gtccggcttc acgctggttt tcggcctgat ggacgtgctc
     3121 aatttcggcc atggcgcctt catctcgatc ggcgcctata tggcgacgct ggtcttcgcg
     3181 ccgctgatcc tttggtcgca ggccggctcg cttgggctca atctcggcgt catctttttg
     3241 gcggccgccg tggccgccat cgtctgcggc gcgctcggcc tcgtcttcga gcgcttgctg
     3301 gtgcggccag tctatggcca gcatctcaag cagattctgg tgacgaccgg cggcctgatc
     3361 gtcgtcgagc aattgctcta cgccctgttc ggtcccgata cgatcccgct ggcgctgccg
     3421 accgccttgc gcggcgcatt cgttatcggc gacgccgcga tcgagaaatt tcggcttctc
     3481 gccgtgatcg tcggcctggt catcttcgcc gggctgagcc tgaccctcaa ctacaccaag
     3541 atcggcctcc tgatccgcgc cggagtcgag aatcgcgaga tggtcgaggc gctcggctat
     3601 cggatcaacc ggctcttcct cgccgtgttt atggccggct cggctttggc cggcttaggc
     3661 ggcgtgctgt gggcgcttta tcgcgagcag gtccacgcct cggtcggcga gagcctgacg
     3721 gtgctcatct tcatcgtcgt catcaccggc ggcctgggct cgatcggcgg ctgctttctc
     3781 ggcgcgacct tggtcgcgct ggtcgccaat tacgccggct ttctcgcgcc gaaaatcgcc
     3841 ctcgtctcca acatcgcgct gatggtcgcg gtgctgctgt ggcggccgcg cggcctctat
     3901 ccggcggggg gacgatgaac cttctttccg gcgatccgcc gcgcggccgc gccctcgcgg
     3961 cgctgcttgc gctcattgtc gtcattctgg cgctggcgcc gttcctgttt ccgggcgcca
     4021 aatcgctgaa tgtcgcggcg acgattctga tctatgcgct gctcgtcgcc tcgtacgatc
     4081 tcctgctcgg ctataccggc atcgtctctt tcgcgcatgt gatgttctac ggcgtcggcg
     4141 cctatggcgt cgcgatcgcg ctttacggcc tggggccggg ctggggcgcg ctgctgatcg
     4201 gcgtcgccgc cgccctgccc gtctcggcgg cgctggctct ggcgatcggc ctcttctcgc
     4261 tgcgcgtgca gacgatcttc ttcgccatgg tgacgctcgc cgcggcctcg gccttcaacg
     4321 tgctcgcctc gcagctcgat tggctgaccg gcggcgagga cggccgctcc ttccaagtcc
     4381 cggcggcttt gcgccccggc tttcatctcg tcagcggccg cgtcttcggc gtcgagatca
     4441 acggccgcat gatcagctac tacctcgtct tcgctgcttg cgcgctgctt ttcctggcgc
     4501 tgctgcgcgt cgtcaactcg ccgttcggcc gcgtgctgca ggcgatccgt gaaaatccgt
     4561 tccgcgccga ggcgctcggc tatcgcatcg tctatcatcg gactctggcg acctgcctct
     4621 cggcgatcgt cgccagcctc gccggggcgc tcaacgcgat ctggcagcat tatgtcgggc
     4681 cggacaccgc cttgaacctc tcgatccaaa tcgacgtgct ggtcatggtg gtgatcggcg
     4741 gcatgggctc gctctacggc gcgatcgccg gcgccgccct cttcgtcatc gcgcgcaact
     4801 acctgcaggg cctgctcggc gcgctgtcgg ccgcggcggc cggcgccggc ctgacgctga
     4861 tcgccggcct gctccatccc gaccgctggc tgatgtggct cggcctgatg ttcatcctcg
     4921 ccgtctattt cgtgccgacc ggcattgtcg ggcggctgcg cgccgaggac aaaggctcag
     4981 gctgagggcg gcgtcgcaaa gaaccagggg tggccgtcgg ggtcgcgcgc cgtataggtg
     5041 cggccataat cgacattttc cggcggcttt tcgatctcgg cgccggcggc gcgcgcccgc
     5101 tcgtaatggg cgtcgacatc ggcgagatag acaaacacgc cctgcgtcgc aacgcccgcc
     5161 tcgcgggcgt tgatcatgcc ccattgcttg ccgccttgcc ccatcatgat cttctgcccg
     5221 tcgagctcca tctcgccatg catgccgccc gacggcgtgc cgacgcgcat gatctctttg
     5281 aagccgaagg cccggctgag ccaatcgagc gcggcgggta cgtcgcggta gaagatgtag
     5341 gggatgatct gggtggggac tttgggctgc gccatcgctg ttctcctcat cgggccgcaa
     5401 gataggcgag gcggcgcggc cagtcttgaa gaaatgttac ctgggcgcgt atgtgactcc
     5461 cttctccccg ctttgcgcgc ttcgcgggga gaagcggttc gctcacagcg ggaagccgcc
     5521 gtcatcgggc agggcgcggc gcatgaacgc ggcgggcggc gcgccggcga attccttgaa
     5581 gtcgcgcgtg agatgcgcct ggtccgcata gccgcaggaa tcggcgagcg cagcaaggct
     5641 cgggacggcg ccgcttcgcg cccggcgcaa ggcgcgatcg aagcgcagga cccggccgag
     5701 cagtttcggc gcgatgccga attcgcgcgc aaagcgcatc gtcagatgct tgcggccgca
     5761 gccgatctct tcggcgagcg cggcgacgga aaggcggccg ccgctcgctt gcaatcgttg
     5821 cgcggcaaaa gcgagatcgg cgggcggcgg ccggcgcgcg cgctcgagcc gctcggccat
     5881 tgccgcttcg aggatagcga ggcgcgcctc ttcgctgttg cacgccatca gacgcccgat
     5941 cgtcgcgcgc gccgccgcgc cgaaaagatc ggccggatcg atcagccggt cgccgatctc
     6001 ggccagcgga aaatcgacga gaagacgggc gccgagcggc gtaagcatcg cctgcgcccc
     6061 ttcttgcgcg cggtcggttt cggtgatcgc gaacgtgctg ctgattcccg tgtaaaaggc
     6121 gccgcccgcg ccataggcgt tgaaggtctt gaccgggtgc tcgacgcgca gctccgcgcc
     6181 gagattgaag acgagcgtcg ccaggccgga aggcggctcg gcgcgccgcg tgaacgcggt
     6241 gtcgcgctcg cggtagacgt tgagccgcgc gacgtaaggc gcgaagcgca cgtccggctc
     6301 aagaaacgcc atccgccacg cgccgcgctc ggaagcgtgc gcgacgcggg aaatgcgaag
     6361 ggcgaaatcg tctgctgtca tggtgcgtct gcgctgcaaa aatcccttct caccgttttg
     6421 cggggagaag gagttctaca ccgccgccct tcgccgctgc aaaaaccgcc gcaccgccgg
     6481 atcggcctcc aggccgatcg cctgatcgta ggcggcttgc gcttcggcgc gctcgccggt
     6541 ccgcgccagc aattcggcgc gggccgccca atagggctga tattgggcga gccgctcgtc
     6601 ccccgccatg gcggcgagcg cggcgaggcc ggcggcggcg ccttgcgcct cggccagcgc
     6661 gacggcgcga ttgatcgcgg cgaccggcga gccggtcacg cggcaaagcg cgtcgtaaag
     6721 cgtaacgatc gccggccagt cgacgccgcg gccgagccgc cgcgcgacat gcgccgattg
     6781 caacgccgcc tccaattggt agcggccgat cgcgccatag ccatgcgcgc ggctcaacaa
     6841 gctttccgcc tccgcgatca tgcgctcgtc ccagcaagcg gggtcctgct cggcgagcgg
     6901 cacaaactcg ccctcctgat cgcgccgcgc gccgcgccgc gcgtcggaat agagcatgag
     6961 cgcgagcagg ccgagcgcct ccgcttcgtt cggcaggagc tccgcgatga gccggccgag
     7021 ccagatcgcc tcctcggcga ggttgcgcct cttcgcttcg gcgccggcgg gatcgctcca
     7081 gccttcggcg aaggcggcgt agatggcggc gagcaccgcg tcgagccgct ccgccatgtc
     7141 ctcgcgctcc ggcgtgcgga agggaatgcc ggcgagcttg atcttggcct tggcgcgcgc
     7201 gagcctttgc cccatcgtcg ccggcgcgac gagaaaggcc gaggcgacgc tggccgcgtc
     7261 gaagccgaga acggtctgga ggatcagcgg cgcgcggatc gaagcttcga tcgccggatg
     7321 ggcgcaggcg aacatcaggc tcaggcgctc gtcgggaatg ggcgcggttt cctcggccat
     7381 cgccgccatc tcctcggcca gcaactgaac atggccgccg gcttcagcgg cggtgcgccg
     7441 ccgccgcgcc gcgtcgatcg agcgccgccg cgcggcggcg agcagccaag cctcgggact
     7501 gcgcggaacg ccgctcttcg gccaatcggc gagcgcggcg gcgaaagcct cggacaaggc
     7561 gtcctcggct cccgcgacat cgcgcgtgcg tgcgctcaag agagcgacga ggcgcccata
     7621 ggaacggcgc gcggcggcgg ccgccgccgc atgcgcctca tgctcgtcgc tgacgccgct
     7681 taagctcaca tcgtccagac cggccggatc tcgactgttc cgtgcgaggc gccggggcag
     7741 cgcgccgccc aggagatcgc tgcgtcaagg tcgggcgctt caatcaggta gtagccgccg
     7801 agttgctcct tggtttcggc gtatgggccg ttgagcactt gcgtcttgcc gttggccatc
     7861 cgcaccgtgg tggcggcggc gaccggctgc aggcggttgc tgttgacgag cgcgccggcc
     7921 tttttcagcg cttcggtata ggccatgtag gcgcccatgg cctgctcgat ctgatccttg
     7981 ggaatcgccg cgaaggcggc ctcgctcgaa tggatcagca gcaaatattg catgacgggt
     8041 ctcctcattt tgagccgcgc ggatgcgctg ctgagccgat gtcgttcgga acggcgcggt
     8101 ttcgacaagc acgcaagatt attttccaag ccgacgcaag cgggcccgag cgtcgacgcc
     8161 caagcgtccg tcccggcgat ttggcatttt gtgggaaaga aacctgcgat ttcaatccgc
     8221 ggtgaaagac gaccgataaa aaattcgcca ggcagtcgaa atcggacggg atcaaacgtc
     8281 aacgctgtgc tcgggcgagc gcttgggcgc atccacggag ggtccgatga ataacaatgt
     8341 ttcggcggtc aaagcggctt atgaagccgt ggctcgcggg caggtggaag tcctcttcgg
     8401 cttgctcgcc gacgatattc gctggcgggc gaccggaccg ggcccgttgg cgggcgaata
     8461 tatcggcaag gccgaaatcg gccgtttttt cggcaagatg ggcgagacct acggggacac
     8521 gttccggctg gatgtggttg acgtcctcgg ctccgctgat cgtgtaatcg tccttacgga
     8581 agaaaaagga gagtatcggg gtcagcccgt cgcgtggcga tctgcgcatg tctacacttt
     8641 cgagaatgaa cgatgcgtca gcttcctgag cttccaggac gattcctaca tcaacttttg
     8701 gagcgcccgg cgcagcggcg tggaatgatg gggccgtctg ctttgcgcat atccgccaag
     8761 cattgctccg cccgcatcgg accggcgccg ccgtccgacg cttgacgatt tcgggccgat
     8821 gcggcccctc cgcgcatcag gcgacgccct cgcgaatggc cgagctgatc cggcgcgcaa
     8881 agaatagccc gcccgacgca gcgcccgcta taatccaatc cacctcgtcc tctcgtcccc
     8941 ggagccctcc tcatgaccga cgtctttctc tgcgaaggcg cgcgcacgcc gatcgggcgc
     9001 tatggcggcg cgctcgcttc cttgcggccg gacgatcttc tcgcgcatgc aatcaaggcg
     9061 gcgctcgcca agacgcccaa gctcgatccc gccgcgatcg acgacgtcta tgccggctgc
     9121 gccaaccagg ccggcgagga caatcgcaac gtcgcgcgca tggcgctgct gcttgccggc
     9181 ctgccgcagg agattccggg cgtgacgatc aaccgtctct gcggctcggg gctcgacgcg
     9241 gtcggcgccg cggcgcgggc gatccgagcc ggcgagatcg atctcgccat agccggcggc
     9301 gtcgaatcca tgtcgcgcgc gcctttcgtg caaggcaagg cgacggaggc cttttcgcgc
     9361 aaggccgaga ttttcgacac gacgatcggc tggcgcttcg tcaatccgct gatgaaggcg
     9421 caatacgggg tcgattcgat gccggagacg gccgagaacg tcgccgagga attccagatt
     9481 tcgcgcgccg accaggacgc tttcgcgttg cgcagccagc agcgcgccgc ggcggcgcag
     9541 aaatatctga ccgatctggg tgtcgatgcc tctagaatcg ccaccatcag ctatggcaag
     9601 gacaagcccg tcaacacggc gcacaccccc gaagcctggg ccgagaaccg ccgcgacgag
     9661 atccagccca ggtctccctg acggcgaggc ggggccagat gcgaggatcg ggctcgattt
     9721 cctcaagctg gtgtggcagc tcgaccacgg gctccg
//
LOCUS       DATGJW010000363         2427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_69534, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000363 DATGJW010000000
VERSION     DATGJW010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2427
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69534"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1366
                     /gene="trmFO"
                     /locus_tag="VKU03_11040"
     CDS             <1..1366
                     /gene="trmFO"
                     /locus_tag="VKU03_11040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HLJ71845.1"
                     /translation="AWQIAKAGVPVVLHEMRPSRGTEAHKTGGLAELVCSNSFRSDDS
                     ETNAVGVIHREMRAMDSLIMAAADVHQVPAGGALAVDRDGFSAAVEAAIRARPGIAIE
                     RGEVAGLPPEDWDSVIVATGPLTSPALAAAIRTLTGEEQLAFFDAIAPIVHRDSIDMD
                     KAWLQSRYDKAGPGGSGADYVNCPLDRNQYEAFVDALLAGEKTEFKDFESTPYFDGCL
                     PIEVMAARGRETLRHGPMKPVGLTNAHNPTVKPYAVVQLRQDNALGTLFNMVGFQTKL
                     KYGEQARIFRMIPGLERAEFARLGGLHRNTFLNSPRVLDPLLRLKAMPRLRFAGQITG
                     CEGYVESAAIGLIAGRMAAAERLGQPISLPPQTTAIGALMNHITGGHIETIDAGPRSF
                     QPMNVNFGLFPPIAEPRVDATGKRLRGPERGLARKRALSARAERDLGAWLAERQSVAS
                     AAAE"
     gene            1557..1970
                     /locus_tag="VKU03_11045"
     CDS             1557..1970
                     /locus_tag="VKU03_11045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020608033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLJ71846.1"
                     /translation="MRFEGGCYCGAVRYVAEGAPMLKAQCHCRECQYFSGGSPNMFMV
                     VPTGGFRYSKGTPKQFARSDLDSPVTREFCAECGAHLITRRPGLDAIILKIGTLDDPS
                     LYGAPKMAIFTIDKQTFHHIPDGLPAFERLPPRAS"
     gene            1986..>2427
                     /locus_tag="VKU03_11050"
     CDS             1986..>2427
                     /locus_tag="VKU03_11050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004109657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HLJ71847.1"
                     /translation="MPAADSLSRIDWIVSSCRLLAATRAELQREKPFHNLTIGTAIHL
                     EPKTAALLLTLAAGGARVVSTGNLNSTQPETVAYLRAHGIDVIGERTSEAQAHERFLD
                     AIIAEQPDLLLDNGGDLFARVAARGYAKLRGGTEETTTGRARLAP"
BASE COUNT          408 a          850 c          786 g          383 t
ORIGIN      
        1 cgcctggcaa atcgccaagg ccggcgtccc ggtcgtcctg cacgaaatgc gaccttcgcg
       61 tggcaccgag gcgcataaga cgggcggcct cgccgagctc gtctgctcca attcgttccg
      121 ctcggacgat tccgagacca acgccgtcgg cgtgatccat cgcgagatgc gcgccatgga
      181 ttcgctgatc atggcggcgg ccgacgtgca tcaggtgccg gccggcggcg cgctggccgt
      241 cgatcgcgac ggcttttccg ccgcggtcga ggcggcgatc agagcccggc cgggaatcgc
      301 gatcgagcgc ggcgaagtcg ccggcctgcc cccggaagat tgggacagcg tcatcgtcgc
      361 caccggcccg ctgacttcgc ccgcgctcgc tgcggcgatc cgcacgctga ccggcgaaga
      421 gcagctcgct tttttcgacg cgatcgcgcc gatcgtccat cgcgattcga tcgacatgga
      481 taaggcctgg ctgcaatcgc gctacgacaa ggccggcccc ggcggctcgg gcgccgatta
      541 cgtcaattgt ccgctcgacc gcaatcagta cgaagccttc gtcgacgcgc tgctggccgg
      601 cgagaagacg gagttcaagg acttcgaatc aacgccctat ttcgacggct gcctgccgat
      661 cgaggtcatg gccgcgcgcg gccgcgagac gctgcgccac ggcccgatga agccggtcgg
      721 cctcaccaac gcgcataatc caaccgtgaa gccctacgcc gtcgtccagc ttcgccagga
      781 caatgcgctc ggcactctgt tcaacatggt cggcttccag accaagctga aatatggcga
      841 gcaagcgcgg atcttccgga tgattcccgg cctggagcgc gccgaattcg cccggctcgg
      901 cgggctgcac cgcaacacgt tcctgaattc gccgcgcgtg ctcgatccgc tgctgcgcct
      961 caaagccatg ccgcggctgc gcttcgccgg ccagatcacc ggctgcgaag gctatgtcga
     1021 gagcgccgcg atcggtttga tcgccggccg catggcggcg gccgagcggc tcggccagcc
     1081 gatttccctg ccgccgcaaa ccaccgcaat cggcgcgctg atgaaccata tcaccggcgg
     1141 ccatatcgag acgatcgacg ccggcccgcg ctcgttccag ccgatgaacg tcaatttcgg
     1201 cctgttcccg ccgatcgccg agccgcgggt cgacgcaacc ggcaagaggc tgcgcgggcc
     1261 ggagcgcgga ttggcgcgca agcgcgcgct atcggcgcgg gcggaacggg acctcggcgc
     1321 ctggctcgcc gagcgacaat ccgtcgcgtc cgccgccgcc gaataagctc cggccgttgt
     1381 agccgcggtc gctgatcgcg gcgggcgcgg agcgccccgt ttcgcgtaaa gcgacggcgg
     1441 aggcggcgcg gtcaacgacc ggcctacatg cccgccaagc ggctgaggcc gatgtccttt
     1501 cgtatcgcgg gctctcgccg taatatgcct tgtgggaaga gccgacgagg gacgcgatga
     1561 ggtttgaagg cggatgctat tgcggggcgg tgcgctatgt cgccgagggc gcgccgatgc
     1621 tgaaggcgca atgtcattgc cgagaatgcc aatatttcag cggcggctcg ccaaacatgt
     1681 tcatggtcgt gccgaccggc ggattccgtt atagcaaggg aacgcccaag caattcgcgc
     1741 gcagcgatct cgacagcccg gtgacgcggg aattttgcgc cgaatgcggc gcccatctga
     1801 tcacccgccg gccgggcttg gacgcgatca tcctgaaaat cggcacgctc gacgatccga
     1861 gcctctacgg cgcgccgaag atggcgatct tcacgatcga caagcagact ttccatcaca
     1921 tcccggacgg cttgcccgct ttcgagcggc tgccgccgcg cgcctcttaa agccgaaacc
     1981 gccgcatgcc ggccgccgac tcgctcagcc gcatcgactg gatcgtttca agctgccgtc
     2041 tgctcgccgc aacgagggcc gagctccagc gcgaaaagcc gttccataac ctgaccatcg
     2101 gcacggcgat ccatctcgag ccgaagaccg cggcgctgct cttgactctc gccgccggcg
     2161 gcgcgcgcgt cgtctcgacc ggcaatctca attcgaccca gccggaaacc gtcgcctacc
     2221 ttcgcgcgca cggaatcgac gtgatcggcg agcggacgag cgaggcgcag gcgcatgagc
     2281 gctttctcga cgcgatcatc gccgagcagc ccgatctgct gctcgacaat ggcggcgatc
     2341 tcttcgcccg cgtcgcggcg cgcggctacg ccaagctgcg cggcggcacc gaggagacga
     2401 cgaccggccg cgcccggctt gcgcctt
//
LOCUS       DATGJW010000364         7944 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_69586, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000364 DATGJW010000000
VERSION     DATGJW010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7944)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7944)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7944
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69586"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..571)
                     /locus_tag="VKU03_11055"
     CDS             complement(<1..571)
                     /locus_tag="VKU03_11055"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016758 - hexosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase 87 family protein"
                     /protein_id="HLJ71848.1"
                     /translation="MAQAEHAAGARPFGPPLARGLPILALCVAALAYAWIAIPLTLVP
                     FTERRTWSGGVVGNDFLAFYSAARLAWGHAADAYNLPRPFAAEAAASGTGMRLPFTYP
                     PLFLLYAAPLAAAPYLPALYAWIVATTAPFALVARRLSGLATLLVALSPPVIQNAIDG
                     QNGALTASLFAGGLLLLTRGRPVLAGIAFA"
     gene            790..2346
                     /gene="rpoN"
                     /locus_tag="VKU03_11060"
     CDS             790..2346
                     /gene="rpoN"
                     /locus_tag="VKU03_11060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase factor sigma-54"
                     /protein_id="HLJ71849.1"
                     /translation="MALAAKLVMRQSQSMVMTPQLLQAIKLLQLPNVELAAYIENELE
                     RNPLLERAEDREPAEADGEAPPQAGEDERDWASDELEVDPAALARNLDTEIDNAFEPD
                     RPAVSPAAPPPQEGHGLAASSWSGVAGPSGDGEAPNLEAYAAAEISLAEHLERQAAIL
                     LPDPADLMIARALIDALDEAGYLSAPLAEIAERLGAPLARVEQILARLQSLEPTGIFA
                     RDLAECLSLQLIERDRFDPAMQALIANLPLLAKRDFTQLRRLCGVDDEDLADMVAEIR
                     RLEPKPGRPFGAAPTQIAVADVFVSTAPDGSWRIELNNDALPRILVNETYAATIKRGK
                     TSEADRLFLATNLQNANWLTNSLEQRARTILKVATEIVRRQDAFFAEGVIGLRPLNLR
                     MIADAIGMHESTVSRVTSNKYMQTPRGLFQMKYFFSTSIASNNIGEAHSAESVRFKIK
                     QMIDAEDPNDVLSDDVIVARLKAADIVIARRTVAKYRDSLRIPSSVDRRREKLRRATP
                     AGHRENLLNV"
     gene            2470..3039
                     /gene="raiA"
                     /locus_tag="VKU03_11065"
     CDS             2470..3039
                     /gene="raiA"
                     /locus_tag="VKU03_11065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HLJ71850.1"
                     /translation="MTLRVSGKNIAIGESLRQHVHDKVTAVIGRYFDGSVSGHVVIAP
                     EGPGYRSDCTLHLSSGMTLQAEGKAQAPYASVDQAVDRIERRLRRYKRRLKEHHPAAN
                     GAETTQGRTVANYVIEAPKEDGEESGEFAPVIVAEGTARLKSLSVASAVEELDLTGAP
                     VVVFQHAGSARVNVVYRRRDGAIGWLDPQ"
     gene            3131..3595
                     /gene="ptsN"
                     /locus_tag="VKU03_11070"
     CDS             3131..3595
                     /gene="ptsN"
                     /locus_tag="VKU03_11070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008982 -
                     protein-N(PI)-phosphohistidine-sugar phosphotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0009401 - phosphoenolpyruvate-dependent
                     sugar phosphotransferase system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PTS IIA-like nitrogen regulatory protein PtsN"
                     /protein_id="HLJ71851.1"
                     /translation="MPLSEILAPDAVIGALRVNSKKQALQELSERAAKVSGLAAREIF
                     DALLQRERLGSTGVGDGIAIPHGKMAKCGRIFGIFARLERPIDFEALDGLPVDLLFLL
                     IAPENAGADHLKALALIARALRDPGRAAKLRMTRDPAALFSLLTQEQTSHAA"
     gene            3626..3895
                     /gene="purS"
                     /locus_tag="VKU03_11075"
     CDS             3626..3895
                     /gene="purS"
                     /locus_tag="VKU03_11075"
                     /EC_number="6.3.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurS"
                     /protein_id="HLJ71852.1"
                     /translation="MRARVIVTLKKGVLDPQGKAIEGALASLGVGGIESVRQGKVFDI
                     EIDAPDQAAARAQLRAACEKLLANLIVENYAVEFVEAEPLPAITS"
     gene            3906..4589
                     /gene="purQ"
                     /locus_tag="VKU03_11080"
     CDS             3906..4589
                     /gene="purQ"
                     /locus_tag="VKU03_11080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004642 -
                     phosphoribosylformylglycinamidine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine synthase
                     subunit PurQ"
                     /protein_id="HLJ71853.1"
                     /translation="MRAAIVLFPGSNRDGDVARAIHRVCGAAPSLVWHQESELPAGAD
                     LVVLPGGFSYGDYLRCGAIAARARIMDAVREHARRGGLTLGICNGFQILCEAGLLPGV
                     LMRNSDMRFVCKMQHLRVENVQTPFTRAYAPGQVLKFAIAHGEGNFEAHEETIKRLEG
                     EGRVAFRYCDAAGRIADEANPNGSTNAIAGIYGGEFNILGLMPHPENLIDPLVGGTDG
                     RGLFESLAA"
     gene            4656..5036
                     /gene="hisE"
                     /locus_tag="VKU03_11085"
     CDS             4656..5036
                     /gene="hisE"
                     /locus_tag="VKU03_11085"
                     /EC_number="3.6.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497830.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004636 - phosphoribosyl-ATP diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosyl-ATP diphosphatase"
                     /protein_id="HLJ71854.1"
                     /translation="MSDSLERLFSSVLAARERDPALSRTSKLFRDGVQKMAKKLAEEA
                     FEIGLDAVQMNRDAVVLESADLLYHLAVVWAECGITPETVLGELDRRERLYGVAEKLP
                     KHAVGAQGRRLTVLHGRRHGAAKS"
     gene            5218..7221
                     /locus_tag="VKU03_11090"
     CDS             5218..7221
                     /locus_tag="VKU03_11090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71855.1"
                     /translation="MNAPLPDPKPAQPLGDAVPPFADRAAPFIADGRQGPDLLGLSAA
                     IQPIVEVIAHREAQTPMAIAIVGPSGAGKSFALDRILAGVDALGAATRSAGGPFLGEI
                     VAVPIDAAAIRHDCVAAIASATYAALVRPGEGGHYAALADEAAHAGADPHVAASKALE
                     RHDEARRRLEAERQARDEAEARRARLNEVVLFETPGSRVDAYARASRGRIEARLRRFE
                     LVDGDPIVSFKDFVRDLAGAGGGSRAGVALRALWAYRSQARLLLSAIVLFALAFGVSQ
                     LHSPAVAAWFAGLGAPGAAVGNWLTAHASGIDYVVAALAILGALALALNLWRAFLFTG
                     SLLRGVRLLNFDLRERGRDLDAALARVNRRVAALTAEAEAAARHAEAAEKRARSRGAA
                     APAPGPTPLFLEPGSAPQAAARAFLSALDKMIAGQPEAGPPPVSVAPAPAIAAPGRIV
                     FALDNLDGLPPAEALALIETVHSLLGRSFVALCAFNPASLRPAFGGDAAWRGRLEKLF
                     QIVFNVGGAGEIGGEGLAARLLAGGAAQAQPPVDARKSALSEPISSAEAAVLTAIAPL
                     AASTPRGVKRFLNLYRLARVGVANRAALALALAAAQSDDVEARAALDRLLAESGDGPL
                     PDPAGPPALLAALRAARALHLGVITGADMREANAAAKRFQLLA"
     gene            7820..>7944
                     /locus_tag="VKU03_11095"
     CDS             7820..>7944
                     /locus_tag="VKU03_11095"
                     /inference="COORDINATES: protein motif:HMM:NF014643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008219 - cell death [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system
                     prevent-host-death family antitoxin"
                     /protein_id="HLJ71856.1"
                     /translation="MRSWPVQDAKARFSEMLETCMREGPQLVTKRGAEAAVLVPAT"
BASE COUNT         1324 a         2729 c         2625 g         1266 t
ORIGIN      
        1 aggcgaaagc gatcccggcc aggaccggcc ggccgcgagt cagcaacaac agaccgccgg
       61 cgaacaggct cgcggtgagc gcgccattct ggccgtcaat ggcgttctgg atcaccggcg
      121 gcgacaaagc gacgagcagc gtggcgagcc ccgacaagcg gcgggcgacc aacgcgaacg
      181 gcgccgtcgt ggcgacgatc caggcataga gcgctggcaa atacggcgcc gccgccagcg
      241 gcgcggcgta aagaaggaaa agcggcggat aggtgaaagg caggcgcatg ccggtcccgg
      301 acgccgccgc ctcggccgca aagggccgcg gcagattgta agcgtccgcg gcgtggcccc
      361 aggccaggcg cgccgccgaa tagaaggcaa ggaagtcgtt gccgaccacg ccgccggacc
      421 aggtgcgccg ctcggtgaac ggaaccaggg tcagcgggat cgcgatccag gcataggcga
      481 gcgccgcgac acaaagagcg aggatcggca agccgcgcgc cagcggcggc ccgaacggcc
      541 ttgcgccggc agcatgctcc gcttgcgcca tgggtccttc aacgcttcgc gcccgctcgg
      601 cgcccgcgaa acccagcaca gcacgaaccg gtaaagcatt gatgattatg cgccgcccta
      661 tagcggcggt taaccctcca agcggctttg atcggccgct tccccatccg gcgggcgctt
      721 atgctatatg caagaaatgc gccgcgcggc cctgcgcggc gccggtcagc gtgagcgtcg
      781 gccccggtca tggctttggc agcgaaattg gtgatgcgtc agagccagtc gatggtgatg
      841 acgccgcagc tcttgcaagc gatcaagctg ctgcaactgc ccaatgttga gctcgccgcc
      901 tatatcgaga acgagctcga gcgcaatccg ctcctcgaac gcgccgaaga ccgcgagccg
      961 gccgaggcgg acggcgaggc gccgccccag gcgggcgagg acgagcggga ttgggcgtcg
     1021 gacgagcttg aagtcgatcc cgccgccttg gcgcgaaatc tcgacaccga aatcgacaac
     1081 gccttcgagc cggatcgtcc cgccgtgtcg ccggcggcgc cgccgcctca ggaaggccat
     1141 ggcctcgccg cctcgtcctg gagcggcgtc gccggaccaa gcggggacgg cgaagcgccc
     1201 aatctcgaag cctacgcggc cgccgagatc agcctcgccg agcatctcga acgccaagcg
     1261 gcgattctgt tgcccgatcc cgccgacctg atgatcgcgc gcgccttgat cgacgcgctc
     1321 gacgaggccg gctacctttc cgctcccctc gccgagatcg ccgagcgcct cggcgcgccg
     1381 ctggcccggg tcgagcagat tctcgccagg ctgcagagcc tggagccgac cggaatcttc
     1441 gcgcgcgatc tcgccgaatg cctttccctg caactgatcg agcgcgaccg gttcgatccg
     1501 gcgatgcagg cgctgatcgc caatctgccg ctgctcgcca agcgcgattt cacgcagctt
     1561 cgccggcttt gcggcgtcga cgacgaggat ctcgcggaca tggtcgccga gatccggcgt
     1621 ctcgagccca agcccggccg tcccttcggc gcggcgccga cgcaaatcgc cgtcgccgac
     1681 gttttcgtct ccacggcgcc ggacgggtcg tggcggatcg agctcaacaa cgacgccctg
     1741 ccgcgcattc tggtcaacga gacttacgcg gcgacgatca agcgcggcaa gaccagcgaa
     1801 gccgaccgct tgttcctcgc cacgaacctg caaaacgcca attggctgac caacagcctg
     1861 gagcagcgcg cccgcaccat cctgaaagtc gcgaccgaaa tcgtgcgccg tcaggatgct
     1921 ttcttcgccg aaggcgtcat cggattgcgg ccgctcaatc tgcgcatgat cgccgacgcg
     1981 atcggcatgc acgaatcgac cgtctcgcgg gtgacgtcca acaaatatat gcagacgccg
     2041 cgcggccttt ttcagatgaa atacttcttt tcgacgtcga tcgcctcaaa caacatcggc
     2101 gaagcgcatt cggccgaatc tgtgcggttc aagatcaagc agatgataga cgccgaggac
     2161 cccaatgacg tactttccga cgacgtcatc gtcgcccggc tgaaagccgc cgacatcgtc
     2221 atcgcacgcc gcaccgtcgc caaataccgc gacagcctgc gcattccctc ctcggtcgac
     2281 cggcgccgcg agaagctgcg tcgggcgacg cccgccggcc atcgcgaaaa ccttctaaac
     2341 gtctgatctc acagtcagtt tacgacttat tttcggccga tttggcgcgc tggattgaca
     2401 caggctcggc aggctcctaa ccgtaaaggc gccatacggc gtcggcggca aagagaggcg
     2461 tgacgacaga tgacgttgcg cgtttcggga aagaacatcg ccatcggcga gtctttgcgt
     2521 cagcacgttc atgacaaggt gacggcggta atcggccgct atttcgacgg cagcgtcagc
     2581 ggccatgtcg tcatcgcgcc ggaaggaccg ggctatcgct cggactgcac tctgcatttg
     2641 agctcgggca tgaccttgca agcggaaggc aaagcgcaag cgccctacgc aagcgtggat
     2701 caagccgttg atcgcatcga gaggcgcttg cggcgctaca agcggcgcct taaggagcac
     2761 caccccgcag cgaacggggc ggagacgacg caggggcgca ccgtcgccaa ttacgtgatc
     2821 gaagcgccaa aagaggatgg cgaggagagc ggcgaattcg cgccggtcat cgtcgcggaa
     2881 gggaccgcgc ggctgaagag cctttccgtc gcctcggcgg tggaggaatt ggacttgacg
     2941 ggcgcgcccg tcgtggtgtt tcagcacgcc ggcagcgcgc gggtgaacgt cgtctaccga
     3001 aggagggacg gcgcgatcgg ttggctcgat ccgcaatagc gcctccggcg ccaaagatgc
     3061 aaggacttaa gtcgccaatt ctccccgcaa gggacagcgg cgcaatcgta agatcaactc
     3121 agagacaagc atgccgctta gtgaaattct ggcgcccgat gcggtgatcg gggcgctgcg
     3181 ggtcaacagc aagaagcaag ctttgcaaga gctgagcgag cgagcggcga aagtgtcagg
     3241 tctggcggcg cgcgaaatct tcgatgcgct tctccaacgc gaaaggctcg gctcgaccgg
     3301 ggtcggcgac ggcatcgcca ttccgcacgg caaaatggcg aaatgcggcc ggattttcgg
     3361 cattttcgcc cggctcgaac ggcctatcga tttcgaggcg ctggacggcc tgccggtcga
     3421 tcttttgttc ctgctgatcg cgccggaaaa cgccggcgcc gatcacctga aagcgctcgc
     3481 cctcatcgcg cgcgctttgc gcgatccggg ccgcgcggcg aaattgcgca tgacccgcga
     3541 tcccgccgcg ctgttctctc tcctgacgca ggagcaaacg tcccatgcgg cttgaccgtc
     3601 gccgcgtcgc cgcgagcgcc ggcgcatgag ggcgcgcgtc atcgtcacgc tgaagaaagg
     3661 cgtcctcgat ccgcaaggca aggcgatcga aggcgcgctc gcctcgctcg gcgtcggcgg
     3721 gatcgagagc gttcgccagg gcaaggtctt cgacatcgag atcgacgcgc ccgaccaggc
     3781 cgccgcccgc gcgcaattgc gcgccgcctg cgagaagctc ctcgccaatc tgatcgtcga
     3841 aaactacgcc gtcgagttcg tcgaggccga gccgcttccc gccataacat cctgaacgcc
     3901 cttaaatgcg cgccgctatc gtcctttttc ccggctccaa tcgcgacggc gacgtcgcgc
     3961 gcgcgatcca tcgcgtctgc ggcgccgccc cgtcgctggt ctggcatcag gagagcgaat
     4021 tgccggccgg cgccgatctc gtcgtcctgc ccggcggctt ctcctacggc gactatcttc
     4081 gctgcggggc gatcgcggcg cgggcccgga tcatggacgc ggtgcgcgag catgcgcgcc
     4141 gcggcggctt gacgctcggc atctgcaacg gcttccagat cctctgcgag gcggggctgc
     4201 ttccgggcgt tctgatgcgc aattccgaca tgcgcttcgt ctgcaagatg cagcacttgc
     4261 gcgtcgagaa cgtccaaacc ccgttcacgc gcgcctatgc gccgggccag gtgctgaaat
     4321 tcgccatcgc tcatggcgag ggcaatttcg aagcccacga agaaacgatc aagcggctcg
     4381 aaggcgaggg ccgcgtcgcg ttccgctatt gcgacgcggc cggccgtatc gccgacgagg
     4441 ccaatccgaa cggctcgacc aacgccatcg ccggaatcta tggcggcgag ttcaacattc
     4501 tcggcttgat gccccatccc gagaacctga tcgacccgtt ggtcggcgga acggacggac
     4561 gcggtctgtt cgagagcctc gccgcctgac ggacccaaac gccgctcggc gcgttttccg
     4621 aatgatttac gatttgcgca gagtatcttg ctcccatgag cgattcgctg gagcgcttat
     4681 tctcgtccgt gctggccgcg cgtgagcgcg acccggcgct gtcgcgtacg tccaagctgt
     4741 ttcgcgacgg cgtgcagaaa atggccaaga agctggccga ggaggcgttc gagatcggcc
     4801 tcgacgcggt gcagatgaac cgcgacgccg tcgtccttga gagcgccgat ctgctctatc
     4861 atctcgccgt ggtctgggcc gagtgcggga tcacgccgga gacggtgctc ggcgagcttg
     4921 accgccgcga gcgcctctac ggcgtcgccg aaaaactgcc gaaacatgcg gtcggcgcgc
     4981 aagggcggcg cctgaccgtg ctgcatggcc gccgccacgg cgccgccaag agctgagctt
     5041 cgcggccaag ccgcgcgacc tttcgtccgt cctccggcca tagaaaagcc gcgccgttcc
     5101 aatcattcgg cgcgtcgtcc ggcggcggct ttgcgcgagc cgcgccagcg gtcataatgc
     5161 cggagcatcg aaggctcgcc cgccgccgcg tcgttgcaat cttcacggag gaaggtgttg
     5221 aacgcgcccc tgcccgatcc caagccggct caaccgctcg gcgacgccgt cccgcccttc
     5281 gccgatcgcg ccgcgccttt catcgccgac gggcggcagg gacccgatct tctcggcctc
     5341 tccgccgcca tccagccgat cgtcgaagtg atcgcccatc gcgaggcgca gacgccgatg
     5401 gcgatcgcga ttgtcggacc atcgggcgcc ggcaagagct tcgcgctcga ccgcatcctc
     5461 gccggcgtcg acgcgctcgg cgcggcgacg cggagcgccg gcgggccgtt cctcggcgag
     5521 atcgtcgcgg ttccgatcga cgcggcggcg atccgccacg attgcgtcgc cgcgatcgcc
     5581 tcggcgacct acgccgcgct cgtccggccg ggcgaaggcg gccattatgc ggcgctcgcc
     5641 gacgaagccg ctcatgccgg cgccgatccg catgtcgccg cgagcaaggc gctggagcgg
     5701 cacgacgagg cgcgccgccg cctcgaagcc gagcgccagg cgcgcgacga ggccgaggcg
     5761 cggcgggcgc ggctgaacga ggtcgtcctg ttcgagacgc ccggctcgcg ggtcgacgcc
     5821 tatgcgcgcg ccagccgcgg ccggatcgaa gcgcggctgc gccgcttcga actggtcgac
     5881 ggcgatccga tcgtcagttt caaggatttc gtccgcgatc tcgccggcgc cggcggcggc
     5941 tcgcgcgccg gcgtcgcgct gcgcgcgctc tgggcctatc gcagccaggc gcggctgctc
     6001 ctgtccgcga tcgtcctctt cgcgctggct ttcggcgtgt cgcaattgca tagcccggcc
     6061 gtcgcggcct ggttcgccgg cctcggcgcg ccgggcgcgg ctgtcggcaa ttggctgacg
     6121 gcgcatgcaa gcgggatcga ctacgtcgtc gcggcgctcg ccattctcgg cgcgctcgct
     6181 ctcgcgctca acctttggcg cgccttcctc ttcaccggca gcctgctgcg cggcgtcagg
     6241 ctgctgaact tcgacctgcg cgagcgcggc cgcgatctcg atgcggcctt ggcgcgagtc
     6301 aatcggcgcg tcgcggcgct gaccgccgaa gcggaagcgg cggcgcgcca cgccgaggcg
     6361 gccgagaagc gcgcccgctc gcggggcgcc gccgcgccgg cgcccgggcc gacgccgctg
     6421 ttcctggagc cgggctcggc cccgcaggcc gcggcccgcg ccttcctcag cgccctggac
     6481 aaaatgatcg ccggccagcc tgaggcgggc ccgccgccgg tcagcgtcgc gccggcgccg
     6541 gcgatcgccg caccaggccg catcgttttc gcgctcgaca atctcgacgg gctgccgccc
     6601 gccgaagccc tggcgctgat cgagacggtc cactcgctgc tcggccgctc gttcgtcgcg
     6661 ctctgcgcct tcaaccccgc ttcgcttcgt ccggcgttcg gcggcgacgc ggcgtggcgc
     6721 ggccgtctcg aaaagctgtt ccagatcgtc ttcaatgtcg gcggcgcggg cgagatcggc
     6781 ggcgaagggc tcgcggcgcg cttgctcgcc ggcggcgcgg cgcaggcgca gccgccggtc
     6841 gacgcgcgga aatcggcgct gagcgagccg atcagttcgg ccgaggccgc ggtgctgacc
     6901 gcgatcgcgc cgctcgccgc ctcgacgccg cgcggcgtca agcgcttctt gaacctctat
     6961 cgccttgccc gcgtcggcgt cgccaaccgc gccgcgctgg cgctcgcgct cgcggcggcg
     7021 cagagcgacg atgttgaggc gcgcgccgcg ctcgaccgct tgctggcgga gagcggcgac
     7081 ggcccgctgc cggacccggc cggcccgccg gccttgctcg ccgccttgcg cgccgcgcgc
     7141 gcgctgcatc ttggcgtcat caccggcgcc gatatgcgcg aagcgaacgc ggcggcgaag
     7201 cggttccagt tgctcgcctg aggaggggcg ggtcgaggcg gccgacgcgc cttcaggggc
     7261 tcgccaaacc cggcgaagcc gacgcatcgc cggcggcggc cgaatttgga aatccttgga
     7321 cggcgcgaaa ataacccacg cgttaaattt ttcgtaccaa aagtttcctc tattatcaat
     7381 ggcgtcgcaa cgcgccgctc caagcttttc caaatttttc tttggtcgtt ttgggcgatt
     7441 tcaataggtt gggcgcgaaa aaagtttgaa atcgcgtttt tctcttcgtc ctctcgatcg
     7501 tacggccgcc acggcgtccc gtctgccatg aagtacgaag tatactcgag acggttagtc
     7561 tggggaagga tttgttccgt cgattagacc agcgttgttt ggaacagggt ctgtgtgcgc
     7621 cggccgggag gattgtcaag gacggctcgc gacatggccg tagatagacg gagcgccttc
     7681 gacgctccta tcgcgagtcg ccgccggagg cggtaattct gcttttcaga aataactttt
     7741 ccacccttgg ccgggcgttg ccaaacccct cgtcaaagac tagtcatctg accagttaga
     7801 ctcccgatgg agaaaggcga tgcgctcctg gcccgtccaa gacgcaaagg cgcgattcag
     7861 tgaaatgctc gagacgtgca tgagggaggg tccgcagctc gtcaccaaac ggggcgccga
     7921 ggccgccgtc ctcgtccctg cgac
//
LOCUS       DATGJW010000365         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1000889, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000365 DATGJW010000000
VERSION     DATGJW010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1000889"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            35..334
                     /locus_tag="VKU03_11100"
     CDS             35..334
                     /locus_tag="VKU03_11100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71857.1"
                     /translation="MSRLRASAAVLLALWLPAAAVAAPSAVHARHHHRHHHDRLLGVA
                     PGLDLYGPSAGYDRGYGSFGGQLYTRNSGGVNSMANDFGTSGVLGHTNGMPATGR"
     gene            413..751
                     /locus_tag="VKU03_11105"
     CDS             413..751
                     /locus_tag="VKU03_11105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71858.1"
                     /translation="MPKLTANGLVILALSLATVSQAAAVQAHVRHHRHALPPSSDIIV
                     RTPPVYYPPGFVSNGVDFGGGGANGLSGDDSYPPPRGFHGGLYSYGVYAMGPGGPYDK
                     VEGCLPGFSC"
     gene            792..1073
                     /locus_tag="VKU03_11110"
     CDS             792..1073
                     /locus_tag="VKU03_11110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71859.1"
                     /translation="MGRIFAVGALASFLVLPEAALALGPGLGLDADLGQLDAGLYPSD
                     PASNAPGAGGVWTAMTGSNFTPAPSPTVRIRGAAPRFRIIRAPRITVTP"
     gene            1139..1531
                     /locus_tag="VKU03_11115"
     CDS             1139..1531
                     /locus_tag="VKU03_11115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71860.1"
                     /translation="MIEAWVLDRKNDSGSLPGWLIAAVRDRTEDGLLVLASRAGTVEA
                     RPGDLVLWDGERIDVYKGQRVGARSERPPAGASRRPSAAADQRSKGRPRRRLSLALVF
                     AVIVFLGLLAFGYAWLMIRLMGHVRPHY"
     gene            complement(1547..2425)
                     /locus_tag="VKU03_11120"
     CDS             complement(1547..2425)
                     /locus_tag="VKU03_11120"
                     /EC_number="3.1.1.99"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018482297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMP-30/gluconolactonase/LRE family protein"
                     /protein_id="HLJ71861.1"
                     /translation="MRIEVLVDVKTTLGEGPLWDAEQERLYWIDSFDGRVFRATAAGS
                     EIRAWDVPQKIGSMALRKDGGGAIVSLQRGFHLLDFASGEVELIVDPEPGKSNNRLND
                     GKVDKRGRFVAGSMDTMEDGPNGALYRLDPDFSLHKLDDGIIVSNGPCWSPDGKTFYF
                     TDTWTGEIWAYDYDQATGAASNRRTFVKVDTSRGGAADGSTVDAEGCVWNALVYDGRL
                     VRYDPSGRIDRIIEMPVKKVTSVMFGGANLDILFVTSMAKPPLPRFPGDGPLRGSLFA
                     IYDLGVKGVPEPRFGA"
     gene            complement(2512..>2635)
                     /locus_tag="VKU03_11125"
     CDS             complement(2512..>2635)
                     /locus_tag="VKU03_11125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0043720 - 3-keto-5-aminohexanoate cleavage
                     activity [Evidence IEA];
                     GO_process: GO:0019475 - L-lysine catabolic process to
                     acetate [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLJ71862.1"
                     /translation="SNAEQVALARKIIEGLGKQVATPDDAREILALKGADHVAF"
BASE COUNT          407 a          880 c          865 g          483 t
ORIGIN      
        1 aaaagaatat tgaatctctt cctgggagaa cgagatgtcc agattgagag cgagcgccgc
       61 tgtgcttctc gcgctctggc ttccggccgc ggcggtcgct gcgccgagcg cggttcacgc
      121 ccggcatcat catcgtcatc accatgaccg attgcttggt gtggcgccgg gtttggacct
      181 ttacggacca tcggcaggct atgaccgcgg ctatggttcg ttcggcggcc agctctatac
      241 gcgaaacagc ggcggcgtga acagcatggc gaacgacttt ggcacgtccg gcgtgctcgg
      301 ccatacgaac ggcatgccgg ccaccggacg gtgatgccga ttgcgctggc ggaacggcgc
      361 ggctgcggta aagttaccct cggcgccaag ggtcgcgcga aggaggacgg gcatgccgaa
      421 gctgaccgcc aatggattgg tgattttggc tctttctctc gccaccgtat cgcaagccgc
      481 agcggtccag gcccatgtcc ggcatcatcg ccatgctttg ccgccgtcaa gcgacatcat
      541 cgtgcgaacc ccgccggtct attatccgcc cggattcgtc agcaacggcg tggatttcgg
      601 cggcggcggc gcgaatgggc tgagcggcga cgattcctat ccgccgccgc gcggctttca
      661 tggcggcctt tacagctacg gcgtctatgc gatggggccg ggcggtcctt atgacaaggt
      721 cgaggggtgc ctgcctggat tctcgtgctg accgacgact cccccgcggc tgatccgaac
      781 ggagaggaaa gatggggagg attttcgcgg tcggcgccct ggcgagcttt ctcgtcctgc
      841 cggaagcggc gctggccctc gggcccgggc tcggcctcga cgccgatctc ggccagttgg
      901 acgccggcct ttatcccagc gaccccgcct cgaacgcccc gggggcgggg ggcgtgtgga
      961 ccgcaatgac cggctcgaat ttcacgccag cgcccagccc aacggttcgc atccgcggcg
     1021 cggcgccgcg tttccggatc atccgggcgc cgcggatcac cgtgacgccc tgacgctccc
     1081 gccgctattg cgcggccggc gcgaatgcgc ttttgattga cgcctcgcgg cgcaagccat
     1141 gatcgaagcg tgggttctcg ataggaagaa cgattccggc tcgctgccgg gatggctcat
     1201 tgcggctgtc agggatcgga ccgaggacgg tctgctggtc ctggccagcc gcgccggaac
     1261 ggtcgaggcg aggcccggcg atctcgtcct ctgggacggc gagcggatcg acgtctataa
     1321 gggtcagcgc gtcggcgcgc gatcggagcg tccgcccgcc ggcgcgtcgc gccggccttc
     1381 ggccgccgca gatcagagga gcaaaggcag gccgcggcgg cgcctcagcc ttgcgctggt
     1441 cttcgccgtc atcgtctttc tcggcctgct ggctttcggc tatgcatggc tgatgatcag
     1501 gctgatgggc cacgtccggc cgcattattg agcgcggccg cagaccctaa gcgccgaagc
     1561 gcggctccgg cacgcccttg acgccgagat cgtaaatggc gaacaagctg ccgcgcagcg
     1621 gcccgtcgcc gggaaagcgc ggcagcggcg gcttcgccat cgaggtgacg aaaaggatgt
     1681 cgaggttcgc cccgccgaac atcacgctcg tcactttctt caccggcatt tcgataatcc
     1741 ggtcgatccg cccgctcgga tcgtacctga ccagcctgcc gtcgtagacg agggcgttcc
     1801 agacgcagcc ctcggcgtcg accgtcgagc cgtccgcggc gccgccgcgc gacgtatcga
     1861 ccttgacgaa agtgcgccgg ttggacgccg cgcccgtcgc ctggtcataa tcgtaggccc
     1921 agatttcgcc ggtccaggtg tccgtaaagt agaatgtctt gccgtccgga ctccagcacg
     1981 gaccgttcga gacgatgatg ccatcgtcca gcttgtgcag cgaaaagtcg gggtcgagcc
     2041 ggtaaagggc gccgttcgga ccgtcctcca tcgtatccat cgagcccgcg acgaagcggc
     2101 cgcgcttgtc gaccttgccg tcgttgaggc ggttgttgct cttgccgggc tctggatcga
     2161 cgataagctc gacctcgccg ctcgcaaaat cgagcagatg gaagccgcgc tgcaacgaga
     2221 cgatcgctcc gccgccgtcc ttgcgcagcg ccatcgagcc gattttctgc ggcacgtccc
     2281 aggcgcggat ctcgcttccc gcggcggtgg cgcggaagac gcggccgtcg aaactgtcga
     2341 tccaatagag ccgctcctgc tcggcgtccc agagcggtcc ttctccgagc gtcgtcttga
     2401 cgtcgacgag aacttcgatt cgcatgatct cctcccgctt gaccggcgcg ccgtcattgc
     2461 ttcgtcgctg cgctcctcgc aatgacggcg atgcgcctca tcggatctgg cctaaaacgc
     2521 gacgtgatcg gcgcccttca gcgcgagaat ctcccgcgcg tcgtccggcg tcgccacctg
     2581 cttgcccagc ccttcgatga tcttcctggc gagggcgacc tgctcggcgt tggac
//
LOCUS       DATGJW010000366         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1313909, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000366 DATGJW010000000
VERSION     DATGJW010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1313909"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..604
                     /locus_tag="VKU03_11130"
     CDS             <1..604
                     /locus_tag="VKU03_11130"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ71863.1"
                     /translation="VFGMDAGASPLIVTNVTSNGYAMAADTAAYVANRINGKGNVVMF
                     VYDAFPPVQARGVIADAIFKNNPDIKVIDRITPDVKDGGIADSRAKMEALLAAHPKKG
                     DIAAVWSAWDQPALGALQAIEAAGRSGEGIVITGIDANPQAREAIAKGGNFEASIAQD
                     FKGIGRTTADAVKRTLAGEKIKQSVIYVPTKLITAANAKD"
     gene            769..1734
                     /locus_tag="VKU03_11135"
     CDS             769..1734
                     /locus_tag="VKU03_11135"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71864.1"
                     /translation="MPLALNIARKYGTLIGVAVILLYFWINLPGTFLTARNLLNISQQ
                     MSMLAVVAFAMTVVMAVGDFDLSVGSTASLAGIVAALVFIAGGPPILAIAAALAAGLA
                     GGLINGFLISYVGILPFVATLGTMTVYSGLAFRLSGGRTLFGSAIPASFADFAREGIP
                     LGGSGLVLPNLTLVALGALALTWLLLEQTVYGRRIYAIGGNSEAARLVGIPVKALRLA
                     AFGLSGLGAAAAGLMYASRVASANPTQGAGLMLDSIAAVFLGTTISEQGEPRVLNTLA
                     GVLILGVLGNGLTQMNVDSYVRDMLTGSIVIVAVAASSLSKLRRR"
     gene            complement(1841..>2079)
                     /locus_tag="VKU03_11140"
     CDS             complement(1841..>2079)
                     /locus_tag="VKU03_11140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007774030.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HLJ71865.1"
                     /translation="EARHLSANALALALRTPSGRITDILNGKRAVSVETAMRLGRYFG
                     QSPRFWLNLQTSYDLAQAETSIGARIDNEVAPAA"
BASE COUNT          350 a          714 c          658 g          357 t
ORIGIN      
        1 ggtattcggc atggacgcag gcgcaagccc gctcatcgtg acgaacgtca cgagcaacgg
       61 atacgccatg gcggccgaca ccgccgccta tgtcgccaac cgcatcaacg gcaaaggcaa
      121 tgtggtgatg ttcgtctatg acgccttccc accggtccag gcccgcggcg tcatcgccga
      181 cgctattttc aagaacaatc ccgatatcaa ggtcatcgac cgcatcacgc ctgacgtgaa
      241 ggacggcggc atcgccgatt cgcgcgccaa gatggaggcg ctgctcgccg cccatcccaa
      301 gaagggcgac atcgcggcgg tgtggtcggc ctgggaccag cccgcgctcg gcgcgctgca
      361 ggcgatcgag gcggcgggac gcagcggcga aggcatcgtg atcaccggca tcgacgccaa
      421 tccgcaggcg cgcgaggcga tcgccaaagg cggcaatttc gaagcctcga tcgcgcagga
      481 cttcaaaggc atcggcagga cgaccgccga cgcagtcaag cgcacgctcg ccggcgagaa
      541 gatcaagcag agcgtgatct atgtgccgac caagctgatc accgcggcca acgcgaagga
      601 ctgagcccat cctcccccgt cgaagacggg ggagggggac cgcgcgtagc gcggtggaga
      661 gggccgccgg cggttgagaa ataatcctga accgccggcg ccccctccac catgcttcgc
      721 atggtccccc tcccccgctg cacgggggag gattttcgcc gcctcacgat gcccctcgcc
      781 cttaacatcg cccgaaagta cggcacgctg atcggcgtcg ccgtgatcct cctctatttc
      841 tggatcaacc tgcccggcac gttcctgacc gcgcggaacc tgctcaacat ctcgcagcag
      901 atgagcatgc tcgcggtcgt cgccttcgcg atgaccgtgg tgatggcggt cggcgacttc
      961 gatttgagcg tcggctcgac ggcgagcctt gccggcatcg tcgcggcgct ggttttcatc
     1021 gccggcgggc cgccgatcct cgccatcgcc gcggcgttgg cggccggcct cgccggcggc
     1081 ctgatcaacg gtttcctgat cagctatgtc ggcattctgc ccttcgtcgc cacgctcggc
     1141 acgatgaccg tctatagcgg cctcgccttc cgcctctccg gcggcaggac tctgttcggc
     1201 agcgccatcc ctgcgtcttt cgccgatttc gcccgagaag ggattccgct cggcggctcc
     1261 ggcctggtcc tgccgaatct cactctcgtc gcgctcggcg cgttggcgct gacctggctc
     1321 ctgctcgaac agaccgtcta tggccgcagg atttacgcga tcggcggcaa cagcgaggcg
     1381 gcgcggcttg tcggcattcc ggtcaaggcg ctgcgcctcg ccgccttcgg cctttccgga
     1441 ctcggcgccg cggccgccgg cctgatgtat gcgagccggg tcgcctccgc caatccgacc
     1501 cagggcgccg gcctgatgct cgattcgatc gccgcggtct tcctcggcac gacgatcagc
     1561 gaacagggcg agccgcgcgt cctgaacacg ctcgccggcg tgttgatcct cggcgtgctc
     1621 ggcaacggcc tgacgcaaat gaacgtcgac agctatgtcc gcgacatgct gaccggatcg
     1681 atcgtcatcg tcgccgtggc ggcgagcagc ctgagcaagc tgcgcaggcg atagcgcgcg
     1741 accgtcattg cgagcgaagc gaagcaatcc gctcgtgaga cgcaatctta tgaatgcatt
     1801 tgcttcgtcg cttcgctcct cgcgatgacg acgtcacgca tcaggcggcg ggggcgacct
     1861 cattgtcgat gcgagcgccg atgctggttt ccgcttgcgc cagatcgtag ctggtttgca
     1921 aattcagcca aaagcgcggg ctctggccga aatagcggcc gaggcgcatc gccgtctcca
     1981 ccgatacggc tcgcttgccg ttcaaaatat cggtaatgcg gccggacggg gtgcgcaacg
     2041 cgagagccaa ggcgttcgcg gacagatgcc tcgcctcca
//
LOCUS       DATGJW010000367         5839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_173411, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000367 DATGJW010000000
VERSION     DATGJW010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5839
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_173411"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..328)
                     /locus_tag="VKU03_11145"
     CDS             complement(<1..328)
                     /locus_tag="VKU03_11145"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ71866.1"
                     /translation="MIDAEARARPSPLDLLWNSPMLLLGLTALMWGGNSVAGRLAVGE
                     ISPMALVFWRWGLACIPLLIAARANLKSDLKVILPRWRYVALAGALGFTAFNALFYVA
                     AHRTSAV"
     gene            complement(552..1451)
                     /locus_tag="VKU03_11150"
     CDS             complement(552..1451)
                     /locus_tag="VKU03_11150"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLJ71867.1"
                     /translation="MGSLKALLIAGGVALAATSVAKAADLLPPAPPVEPVAPPMEFSG
                     WYLRGDVGMALNMTTPKLTDTPNPLVGLPADAFNSFYNPTISGAGLFDIGVGYQFNSW
                     LRFDVTGEYRGGSHFQALEQVGIPSTSTQFADFYRGSLSSYILMANGYVDLGTWYGVT
                     PFVGAGIGWAQNHLDGVTDTGFAYIGPGATGSPTGGFFDNGTTNNFAWALMAGLDFDV
                     TQNLKLELGYRYLSYGTAKTGASHCFNGTGAGGGFSAGSCGGSAHYISTDRLASSDFR
                     LGLRWMISDVPTYAPPPPIVTKY"
     gene            complement(1750..3096)
                     /gene="glmM"
                     /locus_tag="VKU03_11155"
     CDS             complement(1750..3096)
                     /gene="glmM"
                     /locus_tag="VKU03_11155"
                     /EC_number="5.4.2.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008966 - phosphoglucosamine mutase
                     activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucosamine mutase"
                     /protein_id="HLJ71868.1"
                     /translation="MARKYFGTDGIRGRANGKITPEVAMRVGQAAGLAYQRGDHRHRV
                     VIGKDTRLSGYMIEYAMVAGFTSVGMDVLLLGPVPTPAVAMLTRSMRCDLGVMISASH
                     NPFEDNGIKLFGPDGFKLSDEVEAQIEALMDLEIAPRLAKARDLGRAKRIESVQARYI
                     EYAKRTLARNLSLEGLRIVVDCANGAAYKVAPEALWELGAEVFSIGIDPDGFNINHEV
                     GSTAPEALAHKVREMRADIGIALDGDADRVLIVDEKGHIVDGDQLMAVVAESWRDDGR
                     LAKPGIVATIMSNLALERYLAGLGLALERTAVGDRYVLERMRELGYNVGGEQSGHIIL
                     SDYATTGDGLVAALQVLAVVRRENKPVSEICHRFEPLPQILKSVRYKDGRPLERAHVI
                     AAVAAAENELGKSGRLVIRPSGTEPVIRVMGEAEDRDLVERVVNDVCDALAEAAAA"
     gene            complement(3271..5202)
                     /gene="ftsH"
                     /locus_tag="VKU03_11160"
     CDS             complement(3271..5202)
                     /gene="ftsH"
                     /locus_tag="VKU03_11160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HLJ71869.1"
                     /translation="MNPNIRNFALWVIIFLLVLALVTLFQSPGQKAPTTEISFSQLLT
                     EVDQGHVREVTIAGPEISGRFTDNRAFSTYAPSDPSLVDTLYKKNVTINAKPPSDGNS
                     WLMTLLVNGLPLLAFLGVWIFLSRQMQGAGGKAMGFGKSKAKLLNEAHGRVTFEDVAG
                     VDEAKEDLQEIVEFLRDPQKFQRLGGRIPRGVLLVGPPGTGKTLTARAVAGEANVPFF
                     TISGSDFVEMFVGVGASRVRDMFEQAKKNAPCIIFIDEIDAVGRHRGAGLGGGNDERE
                     QTLNQLLVEMDGFEQNEGIIIIAATNRPDVLDPALLRPGRFDRQVFIPNPDVVGREKI
                     LKVHVRKVPLSPDVDLKVVARGTPGFSGADLMNLVNEAALMAARRGKRIVTMAEFEDS
                     KDKIMMGAERRTLVMTEQEKMLTAYHEGGHAIVALNVPATDPVHKATIIPRGRALGMV
                     MQLPERDKLSMSYEQMTSRLAVLMGGRVAEEIIFGKDKVTSGAQSDIEQATKLARAMV
                     TRWGFSDELGTVMYGENQEEVFLGYSVGRQQNISEATSQKIDSEVRRLVEQGLAEATR
                     ILTERRADLETLARGLMEYETLTGEEIDGLLKHGRPPVRETGEKETPPPRGSAVPATA
                     KPRPSPPPAPGGLEPQPQT"
     gene            complement(5340..>5839)
                     /locus_tag="VKU03_11165"
     CDS             complement(5340..>5839)
                     /locus_tag="VKU03_11165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HLJ71870.1"
                     /translation="SELVAYCRAEGEAYFEDPANEDPRFARTRLRRLAALLAEEGFGP
                     AEAARLSRRAARMEEAVLRATEAAASRLNLQTGGGLDALALFAEPPEVALRLLKAAIL
                     AAGGREAAAARLEQIEALAEALQGKVAAGEALRTTLGGLSLRLDAKGVLRIDKAPPRR
                     RSGKP"
BASE COUNT         1013 a         1867 c         1948 g         1011 t
ORIGIN      
        1 tgacggcgct ggtccggtgg gcggcgacat agaaaagcgc gttgaacgcg gtgaagccga
       61 gcgcgccggc gagcgcgaca tagcgccagc gcggcaagat gactttcagg tccgacttca
      121 aatttgcgcg agcggcgatg aggagcggga tgcaggcgag gccccagcgc caaaagacga
      181 gcgccatcgg cgaaatctcc ccgaccgcga ggcgcccggc cacggaattg ccgccccaca
      241 tcagggcggt caggccgagc aggagcatcg gcgaattcca aaggaggtcg agaggagaag
      301 gccgcgcgcg ggcttcggcg tcgatcatcg ggcgcaatct ctccttaagg cgcgccgcgg
      361 cgatctcggg gcctcatccg accaatcgct tacagtctga cgggccgcca aggcaatgtc
      421 gcggcggccg ggcatggcag agatgcgcgc ctcgcgacgc cgattgtggc cgttcgagcg
      481 aggcgccggc caaaaaaaga acggcgccgg ggagatccgg cgccgctcgg ctgacgagga
      541 tggcaggggg atcagtattt ggtcacgatc ggcggcggcg gcgcgtaggt cggcacgtcg
      601 ctgatcatcc agcgcaggcc gaggcggaag tccgacgaag cgaggcggtc ggtcgagatg
      661 taatgggccg agccgccgca cgagccggcg ctgaagccgc cgccggcccc ggtgccgttg
      721 aagcagtgcg acgcgccggt cttggccgtg ccgtagctga gatagcggta gccgagttcg
      781 agcttcagat tctgcgtcac gtcgaaatcg agaccggcca tcaacgccca ggcgaaattg
      841 ttcgtcgtgc cgttgtcgaa gaagccgccg gtcggcgagc cggtcgcgcc ggggccgata
      901 taggcgaagc ccgtatcggt gacgccgtcg agatggtttt gcgcccagcc gatgccggcg
      961 ccgacgaatg gcgtgacgcc gtaccacgtt ccgagatcga catagccgtt ggccatgagg
     1021 atataggagg agagcgagcc gcggtagaaa tcggcgaact gcgtcgaggt cgaaggaatg
     1081 ccgacctgct ccagcgcctg gaaatgcgag ccgccgcgat attcgccggt tacatcgaag
     1141 cgcagccagc tattgaactg atagccgacg ccgatatcga agagcccggc gccggaaatg
     1201 gtcggattat agaagctgtt gaacgcgtcg gccggcaagc cgacgagcgg attgggcgta
     1261 tcggtgagct tcggcgtcgt catgttcagc gccatgccga catcgccgcg caagtaccag
     1321 ccggaaaatt ccatcggcgg cgcgacgggt tcgaccggcg gcgccggcgg caggagatcg
     1381 gcggccttgg cgacacttgt cgcagcgagc gcgacgccgc cagcaattaa aagggctttc
     1441 aagctgccca tgacaacttc cctcattcag cgcgcattcg cgatgcgctc gatgaggcaa
     1501 gctctcatga agtgtttaac agccgcttaa ccctaataat tgaccttaac ggcgcgttgc
     1561 aattatggac cgcgcccctc tgccgcgggt gggaatctcg aaagatttta acaggccggg
     1621 cgccgaatat tgcggcagaa tgaccattct ggccgtctcg agcgtggccc gatgtggcgt
     1681 cttgtcgcgc ctctgatccg tcatggcgag gagggcggcg acgaagcaat ccacggccga
     1741 gcgtgtccgc tacgcggcgg ccgcttcggc aagcgcgtcg caaacgtcgt tgaccacgcg
     1801 ctcgacgaga tcgcggtcct ccgcttcgcc catcacgcga atcacgggct cggtgcccga
     1861 cggtcgaatg acgagacggc cgcttttgcc gagctcgttc tcggccgccg cgaccgcggc
     1921 gatgacgtgc gcccgctcga gcggccggcc gtccttgtag cggacgcttt tcaagatttg
     1981 cggcagcggc tcgaagcgat ggcagatttc gctgaccggc ttgttctcgc gccggaccac
     2041 ggcgaggacc tgcagcgccg cgacgaggcc gtcgccggtc gtcgcgtagt cggagagaat
     2101 aatatggccg gattgttcgc cgccgacatt gtagccgagc tcgcgcatgc gctcgagcac
     2161 gtagcggtcg ccgaccgcgg tgcgctccaa tgcgagcccc aatcccgcca gatagcgctc
     2221 cagcgcgagg ttggacatga tcgttgcgac aatgcccggc ttggcgaggc gtccgtcgtc
     2281 gcgccagctt tcggcgacca ccgccatcaa ttgatcgccg tcgacgatgt ggcccttctc
     2341 gtcgacgatc agcacgcggt cggcgtcgcc gtcgagggcg atgccgatat cggcgcgcat
     2401 ctcgcgcacc ttatgggcga gcgcttccgg cgccgtcgag ccgacttcgt gattgatatt
     2461 gaacccgtca ggatcgatgc cgatcgagaa cacttcggcg ccgagctccc ataacgcttc
     2521 cggcgcgacc ttgtaggcgg cgccgttggc gcaatcgacg acgatgcgca agccttcgag
     2581 cgacagattg cgcgccaacg tccgcttggc gtattcgatg tagcgggcct gcacgctttc
     2641 gatgcgcttg gcgcggccaa ggtcgcgcgc cttggcgagg cgcggcgcga tttcgaggtc
     2701 catcagcgcc tcgatctgcg cttcgacttc gtccgaaagc ttgaatccgt ccggcccgaa
     2761 cagcttgatg ccgttgtcct cgaacggatt gtgcgaggcg gagatcatca cgccgagatc
     2821 gcagcgcatc gagcgggtca gcatcgcgac ggccggcgtt ggaaccggtc cgagcagcag
     2881 cacgtccatg ccgacggagg tgaaaccggc caccatggcg tattcgatca tgtagccgga
     2941 gagccgcgtg tccttgccga tgacgacgcg gtggcgatgg tcgccgcgct gataggcgag
     3001 gcccgccgcc tggccgaccc gcatcgcaac ttccggggtg attttgccgt tggcccggcc
     3061 gcgaatgccg tcggtgccga aatacttgcg cgccatcgct gtttaaacct tcgatcgcgg
     3121 ccccttcgcc gcgatcattt aacgcgaatt tacgtcgaaa atcattcaag aatcattgcc
     3181 gggagttaat gctggcgctc ccttctcccc gcaaagcggg gaaaaaggga ttaagtcttc
     3241 cggcaaagcg cgcaaagcgg ggagaagggg ttaagtctgc ggctgcggct cgagaccgcc
     3301 gggggcgggc ggcggcgacg ggcggggctt ggcggtcgcg ggcaccgccg agccgcgcgg
     3361 cggcggcgtc tctttttcgc ccgtctcgcg aaccggcggc cggccatgct tcaacaggcc
     3421 gtcgatctct tcgccggtca gcgtctcgta ttccatcagg ccgcgagcca gcgtttcgag
     3481 atcggcgcgc ctctcggtca agatgcgggt cgcctcagcg agcccctgct cgaccagccg
     3541 gcgcacttcc gaatcgatct tctgcgacgt cgcctcggag atgttctgct gccggccgac
     3601 cgaatagccg aggaacactt cctcctggtt ttcgccgtac atgaccgtgc cgagctcgtc
     3661 ggagaagccc caacgcgtga ccatggcccg cgccagcttg gtcgcctgct cgatatccga
     3721 ttgcgcgccg gacgtgacct tgtccttgcc gaagatgatt tcttcagcga cgcggccgcc
     3781 catcaatacg gcaaggcgcg aggtcatctg ctcgtagctc attgagagct tgtcgcgttc
     3841 cggcaattgc ataaccatgc cgagcgcgcg gccgcggggg atgatcgtcg ccttgtggac
     3901 gggatcggtc gccggcacgt tgagcgcaac gatggcgtgg ccgccttcgt gataggcggt
     3961 cagcatcttc tcttgctcgg tcatgacgag ggtgcgccgc tcggcgccca tcatgatctt
     4021 gtccttggaa tcctcgaact ccgccatggt gacgatgcgc ttgccgcgcc gcgccgccat
     4081 caaagcggct tcgttgacga ggttcatcaa atcggcgccg gaaaagccgg gcgtgccgcg
     4141 cgcgacgact ttcagatcga cgtcgggcga gagcggcact ttgcgaacat gaaccttcag
     4201 aattttctcg cggccgacca cgtccgggtt cgggatgaag acctggcggt cgaaacggcc
     4261 gggcctgagc agcgccgggt cgagcacgtc gggccgattg gtcgccgcga tgatgatgat
     4321 gccttcgttc tgctcgaagc cgtccatctc gacgagcaat tggttgagcg tctgctcgcg
     4381 ctcgtcgttg ccgccgccca gcccggcgcc gcgatgacgg ccgaccgcgt cgatctcgtc
     4441 gatgaagatg atgcagggcg cgttcttctt ggcctgctcg aacatgtcgc ggacgcggct
     4501 tgcgccaacg ccgacgaaca tctcgacgaa gtcggagccg gaaatcgtga agaacggaac
     4561 gttggcttcg ccggcgacgg cgcgcgcggt caaggtcttg ccggtgccgg gcggaccgac
     4621 gagcaggacg ccgcgcggaa tgcgtccgcc gaggcgctgg aacttctgcg gatcgcgcag
     4681 gaactcgacg atctcctgca agtcctcctt ggcctcgtcg accccggcga cgtcctcgaa
     4741 cgtcaccctt ccatgcgcct cgttgagcag cttggccttg gacttgccga agcccatcgc
     4801 cttgccgccg gcgccttgca tctggcgcga taggaaaatc catacgccga ggaaggcgag
     4861 cagcggcagg ccgttgacga gcagcgtcat cagccaggaa ttgccgtccg acggcggctt
     4921 ggcgttgatc gtgacgttct tcttgtagag cgtgtcgacc agcgagggat cgctcggcgc
     4981 ataagtcgag aaggcgcggt tgtcggtgaa gcggccgctg atttccgggc cggcgatcgt
     5041 cacttcgcgg acatggccct ggtcgacctc ggtgagcaat tggctgaagg aaatttcagt
     5101 cgtcggcgcc ttctggccag ggctctggaa cagggtcacc aacgccagga cgagcaaaaa
     5161 tatgatcacc cagagcgcga aattgcggat attcggattc atcgccgtcc tgtcggcagc
     5221 gtcccggccc aggtctggtc aggcggctct gctgcgttga ctcttcatct aggcgcgccg
     5281 gccccaaaag ccaagggagc cccattactc tgaattagtc gtgaagaagt tgagcaaaat
     5341 tacggtttcc cacttcgccg ccgcggcggc gccttgtcaa tcctcaagac ccccttggcg
     5401 tcgaggcgca ggctgaggcc gcccagggtg gtcctgagcg cctcgccggc ggcgactttg
     5461 ccctgcaaag cctcggccag ggcctcgatc tgctccagcc gcgccgcggc ggcctcgcgg
     5521 cccccggccg ccaggatcgc cgccttgagc aggcgcaaag ccacttccgg aggctcggcg
     5581 aacagagcca gggcgtccaa gccgccgccg gtttgcaaat tcaaccgcga agcggcggct
     5641 tcggtcgccc gcagcacggc ttcctccatc cgcgccgccc gccgcgacag ccgggccgct
     5701 tcggccggtc cgaagccttc ctcggccaga agggcggcga ggcggcgcag ccgcgtcctg
     5761 gcgaagcgcg ggtcctcgtt ggctgggtcc tcgaaatagg cctcgccctc ggcgcggcaa
     5821 taggcgacga gctcgctct
//
LOCUS       DATGJW010000368         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_276627, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000368 DATGJW010000000
VERSION     DATGJW010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_276627"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..270
                     /locus_tag="VKU03_11170"
     CDS             <1..270
                     /locus_tag="VKU03_11170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71871.1"
                     /translation="ATAVYALAGDADYAAGDAFRTLALRHEEILAAYRAGDFAVAASK
                     AAAAESVAPDDLKGLYRTYYKQRFAELAAAGVGADWESVLRLETK"
     gene            complement(372..1637)
                     /locus_tag="VKU03_11175"
     CDS             complement(372..1637)
                     /locus_tag="VKU03_11175"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HLJ71872.1"
                     /translation="MELSDHLFRREAGRMTAALARIFGVHNLGLAEDVVQEAFCRAAE
                     IWKFRGVPDNPSAWLMATAKNCALDALRRQRTARAYAPELGRYLESEWTRAAIIEEQF
                     GANAIKDDLLRMMFSCCQPRLSEEAQIALILRILCGFSVAEIAGAFVSSQAAIEKRIT
                     RAKKVLAESQKLFDVAVAAEFSARLPAVQRALYLLFNEGYHGACAEAAIRTELCREAM
                     RLTAELLVHPLGRTPATYALAALMALDAARLSARADATGALLALADQDRSQWDQQLIG
                     EGLKLLDLSAEGDDLTPYHIEAAIAAAHARARSAEETDWAMIVSLYDSLLAIRPSPIV
                     ALNRAIAVAEKDGPERGLEAIAAIDGRERLDAYPFYPAALGEMALRRGDKTAAAAHFR
                     AALALARNPMERRFLDRRIGVCGPGEERG"
     gene            complement(1639..1989)
                     /locus_tag="VKU03_11180"
     CDS             complement(1639..1989)
                     /locus_tag="VKU03_11180"
                     /inference="COORDINATES: protein motif:HMM:NF015735.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLJ71873.1"
                     /translation="MSEFTFLFRGRDYFASPEQRQKHTEKWVAWFKELGAKGHIKDLG
                     RPLDAAGKVVDKKSVHDGPYAEAKDVVGAYTLIEAADLNQAVELSKGCPILELGGCVE
                     VRPVQVQNVMSLNA"
     gene            complement(2074..2451)
                     /locus_tag="VKU03_11185"
     CDS             complement(2074..2451)
                     /locus_tag="VKU03_11185"
                     /inference="COORDINATES: protein motif:HMM:NF024092.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nuclear transport factor 2 family protein"
                     /protein_id="HLJ71874.1"
                     /translation="MIERAWAEAFARDWIDAWNAHDLARILAHYADDFEMTSPLIVQR
                     LGVAAGKLKGKEAIRAYWGQGLAAAPNLHFTLLDVMVGVNSLAILYESATLSRVVVER
                     IEFDAEGRGARAEALHGPPRPPP"
     gene            2605..>3274
                     /locus_tag="VKU03_11190"
     CDS             2605..>3274
                     /locus_tag="VKU03_11190"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLJ71875.1"
                     /translation="MPAEQAIIGDLVLPDRVLPAAAIVHRGGVIVDIREADKARRDCK
                     VIDHSGRYVSPGFVDIHVHGGANADYMDGAVEAVVSVNQAHARHGTTTLFPTTTTGTS
                     VQLEAMISACETARDSWTPGKGARIGGVHFYGPYFAPDKVGVHSLHGRRDPDREEYER
                     FLSRDIVRIATCAAELPGAGDFYRFAHSRGLLITCGHSNAEWSELERAYAAGMRHVDH
                     FWCAM"
BASE COUNT          548 a         1095 c         1083 g          548 t
ORIGIN      
        1 gccacggcgg tttatgcgct cgccggggac gccgattatg cggccggcga tgcgtttaga
       61 acgctggcgc tgcggcatga ggagattctc gccgcctatc gggcgggaga tttcgccgtc
      121 gccgcgagca aggcggcggc cgccgagagc gtagcgcccg atgacctgaa gggactatac
      181 cggacatact acaaacagcg atttgccgaa ctggccgccg ccggcgtcgg ggctgattgg
      241 gaatccgtat tgcgactcga aacaaagtga acggcgcctg gcgacccgct aaaagattcc
      301 ctcggccggc ccggtccttg cggggctcgc agaaggcgcg cgaggcgccg cgcggcggac
      361 ttttttgagt ttcagccgcg ctcttcgccc ggcccgcaga cgccgatccg ccgatccaga
      421 aagcgccgct ccatgggatt gcgcgcgagc gccagcgcgg cgcggaaatg cgcggcggcg
      481 gccgtcttgt cgccgcggcg cagcgccatc tcgccgagcg cggcggggta gaagggataa
      541 gcgtcaagcc gctctcgccc gtcgattgcg gcgatcgctt cgagcccgcg ctccgggccg
      601 tccttctcgg cgacggcgat ggcgcggttg agcgcgacga tcggcgacgg acggatcgct
      661 agcagcgaat cgtaaagcga gacgatcatc gcccaatcgg tttcctcggc cgagcgggcc
      721 cgcgcatgcg cggcggcgat cgccgcttcg atatggtacg gggtcaagtc gtcgccctca
      781 gccgacagat cgagcagctt caagccctcg ccgatgagtt gctggtccca ttgtgagcgg
      841 tcctggtcgg cgagcgcgag cagcgcgccg gtcgcgtcgg cgcgcgccga aagccgcgct
      901 gcgtcgagcg ccatcaacgc ggcgagcgca taagtcgccg gcgtccgccc gagcggatgg
      961 acgagaagct cggcggtcag ccgcatcgcc tcgcggcaaa gctcggtcct gatcgccgcc
     1021 tcggcgcagg cgccgtgata gccctcgttg aacaagagat agagcgcgcg ctgcaccgcg
     1081 ggcaggcgcg ccgaaaactc ggccgccacc gcgacgtcga agagtttctg cgattcggcg
     1141 aggactttct tcgcccgcgt gatccgtttc tcgattgccg cctggctgct gacgaaggcg
     1201 ccggcgatct cggcgacgct gaagccgcaa agaatgcgga gaatgagcgc gatctgcgcc
     1261 tcttccgaaa gacgcggctg gcagcacgag aacatcatcc gcagcagatc gtccttgatc
     1321 gcattcgcgc cgaactgctc ctcgatgatc gcggcgcgcg tccactcgct ttcgagatag
     1381 cggccgagct caggcgcgta ggcgcgcgcc gtgcgctggc ggcgcagcgc gtcgagcgcg
     1441 caattcttgg cggtcgccat caaccaggcg gacggattgt ccggaacgcc gcggaatttc
     1501 cagatttccg ccgcccggca gaaggcttcc tgcaccacgt cttccgccag gccgagattg
     1561 tggacgccga atatccgcgc caaggcggcg gtcatgcgtc ccgcctcgcg gcggaagaga
     1621 tggtcgctca gctccatttt aagcgttcaa cgacatcacg ttctggactt gcaccgggcg
     1681 cacctcgacg cacccgccga gttcgaggat cgggcagcct ttcgacaatt cgaccgcctg
     1741 gttgagatcg gcggcctcga tcagcgtata ggcgccgacc acgtccttgg cctcggcata
     1801 ggggccgtca tggacgcttt tcttgtcgac caccttgccg gccgcgtcca gcggccggcc
     1861 aaggtccttg atatgcccct tggcgccgag ctccttgaac caggcgaccc atttctcggt
     1921 gtgcttctgc cgctgctcgg gcgaggcgaa atagtcgcgg ccgcggaaaa ggaaggtgaa
     1981 ttcgctcatg atcggctcct tgtcaggatg ttgacggctt aaagacgttc ggcggccgcc
     2041 gcggcggaca tatttctcgc gcctcggcgc ggctcatggc ggcgggcgcg gcgggccatg
     2101 gagggcctcg gcgcgcgccc cgcgcccctc cgcgtcgaac tcgatgcgtt cgaccacgac
     2161 ccgcgacaat gtcgcgctct catagaggat cgccagcgaa ttcacgccga ccatgacgtc
     2221 gagcagggtg aaatgcaggt tcggcgccgc ggcgaggccc tggccccaat aagcgcgaat
     2281 ggcctccttg cctttgagct tgcccgcagc gacgcccagc cgctgcacga tgagcgggct
     2341 cgtcatctcg aaatcgtcgg cgtaatgcgc cagaatccgc gcgagatcat gcgcgttcca
     2401 ggcgtcgatc cagtcccgtg caaacgcttc cgcccaggcg cgctcgatca tccgccctcc
     2461 gcttctccgc aaatgatgtt ggccgcgccg aacctatcag cgcccgggca aggcgacaaa
     2521 ggcgcccccc gcgacgaggc tcgcttcgcg cgaaccgccc atcgtataag acaaccattt
     2581 gtgcaaaatg ggagcggtct ggcgatgccg gcggagcaag cgatcatagg cgacctggtc
     2641 ttgccggacc gcgttctgcc ggcggcggcg atcgtccatc gcggcggcgt catcgtcgac
     2701 atccgcgaag cggacaaggc gcggcgggat tgcaaagtca tcgaccattc tggacgctac
     2761 gtatcgccgg gcttcgtcga catccatgtc catggcggcg ccaacgctga ctacatggac
     2821 ggcgcggtcg aggcggtcgt ctcggtcaat caggcgcatg cgcgccatgg gacgacgacg
     2881 ctctttccga ccacgacgac cggaacgagc gtccagcttg aagcgatgat ctcggcctgc
     2941 gaaacggcgc gggattcctg gacgcccggc aagggcgcgc ggatcggcgg cgtccatttc
     3001 tatggaccgt atttcgcgcc cgacaaggtc ggcgttcatt cgctgcacgg gcgacgcgac
     3061 cccgatcgcg aggaatacga gcgcttcttg agccgcgaca tcgtccgcat cgccacctgc
     3121 gcggccgaac tgcccggcgc cggcgatttc tatcgttttg cgcatagccg cggtctgctg
     3181 atcacctgcg gccattccaa cgccgaatgg tcggagctcg aacgcgccta tgccgcgggc
     3241 atgcgccatg tcgatcattt ctggtgcgcg atga
//
LOCUS       DATGJW010000369         4366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1314595, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000369 DATGJW010000000
VERSION     DATGJW010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4366
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1314595"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            115..191
                     /locus_tag="VKU03_11195"
     tRNA            115..191
                     /locus_tag="VKU03_11195"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:149..151,aa:Pro,seq:tgg)
     gene            216..518
                     /locus_tag="VKU03_11200"
     CDS             216..518
                     /locus_tag="VKU03_11200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004442361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ETC complex I subunit"
                     /protein_id="HLJ71876.1"
                     /translation="MTAKIYQPSRTAMQSGQAKDKWLLEYEPETPRQIEPLMGWTSST
                     DMKSQIKLRFDTKEEAIDYAQRAGIPYRVEEPKPAIRKIISYSDNFKPNRATPWTH"
     gene            528..605
                     /locus_tag="VKU03_11205"
     tRNA            528..605
                     /locus_tag="VKU03_11205"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:563..565,aa:Arg,seq:tct)
     gene            712..1242
                     /locus_tag="VKU03_11210"
     CDS             712..1242
                     /locus_tag="VKU03_11210"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HLJ71877.1"
                     /translation="MPAISKNKPYRIGRSRTGLGLFATKPIKKGTRIIRYFGPLLDAR
                     KDEDVVENKYLFALNSRWTIDGSVRRNIARYINHSCKPNAESVVKPRKRRVFIHAIKN
                     IEKDEEINYDYGAEYFKTYLKPIGCRCAACEESVQAQAKRARLKSSREKNGRTKSRKP
                     AKKAAGARARPRNGSR"
     gene            complement(1357..1929)
                     /locus_tag="VKU03_11215"
     CDS             complement(1357..1929)
                     /locus_tag="VKU03_11215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71878.1"
                     /translation="MIRTVSAAVLAASLAALPALAADNDTSNAPSSAPAQPATTNPPP
                     ANPAPSAQNPPPAAQPANSTPATPTPAPTPSAQNPTSTPPPANPPTPAPTPSAQTPAQ
                     PHAPAPSSNAGQNEQNQAPAAPAAAKSAPASNTAAQPATSAAHRAAPHRPARHHYWRS
                     WRPHHHWRWWRRHRRWIRHPKEEHEPAPRS"
     gene            complement(1992..2786)
                     /locus_tag="VKU03_11220"
     CDS             complement(1992..2786)
                     /locus_tag="VKU03_11220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006469899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71879.1"
                     /translation="MRHLDSSLLLLILLCVAAAVGSFVNSRLTEKHRSRDTIELVQLG
                     ITLLVTFTAIVLGLLTTSVKSGYDMAYAARGAYAGQLAQFERCLRDYGPETQKMREQL
                     RSYVAAVIASTWPSEPRPKGVTYPDVAHLPRIGETPVLADLMDDIGRELYALEPTDTL
                     HHNLMAACAGEYAELLRRRWAVIEGLRGSISSPFYWVLVFWLVILFASFGLRAPPNRM
                     SAIVIALCALSVTVAVFVILDLDLPYGGLFGIPSTAMRNALADMMR"
     gene            3169..3618
                     /locus_tag="VKU03_11225"
     CDS             3169..3618
                     /locus_tag="VKU03_11225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566648.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6481 family protein"
                     /protein_id="HLJ71880.1"
                     /translation="MPNFFGQNFKDRQTAAAEAKKALAAKFLSKPPFNPEDPAYVERQ
                     KQRRAIIEAREEREAQRAAERAAKAAAEAARRAEEERRREAERLEQERLAAEARKAEE
                     ELRELQRQEEAKRKAEIEAQKKAERDARYAARKARKAARKAELKRYY"
     gene            complement(3694..4191)
                     /locus_tag="VKU03_11230"
     CDS             complement(3694..4191)
                     /locus_tag="VKU03_11230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLJ71881.1"
                     /translation="MIRFALIALGALALLPGLAMAKTFPVPADDPIATVSLPDSWNPE
                     PYEGGVEATSDDGAIYVAIEQVAADDVKKATEEGIKFFAKSGVDIDANSMKTKQIKIN
                     SLDAFDINFTGKDKDGPANISLTLVATNAAGKFLLLYYWGSPDGEKANGADLKAISDS
                     IQATK"
BASE COUNT          850 a         1360 c         1450 g          706 t
ORIGIN      
        1 cgcgatcata ttagccgcgc ggccgccgcg agcaagctga atttgcgccg tgcgcctccg
       61 cgcggcgaag tcggggttgt cgacgcagag cgcccatgtt agccacgcca gtctcggggt
      121 atagcgcagc ccggtagcgc ggaagttttg ggtacttcag gtcgcaggtt cgaatcctgc
      181 tgccccgacc acgagcaggc gcctggaaac tgacgatgac cgcaaaaatc taccagccaa
      241 gccgcaccgc catgcaatcg ggccaagcca aggataaatg gctgctcgaa tatgagcccg
      301 agacgccgcg ccagatcgag ccgctgatgg gctggaccag ttcgacggat atgaagtcgc
      361 agatcaagct gcgcttcgac accaaggagg aggcgatcga ctacgcgcag cgcgccggca
      421 tcccctaccg cgtcgaggag ccgaagccgg cgatccgcaa gatcatttcc tattcggaca
      481 atttcaaacc gaatcgcgcc acgccctgga cgcactagac gcctttcgac cccgtagctc
      541 agccggatta gagcagccgc cttctaagcg gcaggtcgca ggttcgagcc ctgccggggt
      601 cgccaagaat ccgttcttgc gcgcatcgaa aaagacggac gcgatcggcg cgtccgccgg
      661 agctagtttt gccgttcctc cgatcttcgc tcgaagccgc ccaaaccggt tatgcccgca
      721 atttcgaaga acaagcccta ccgcattggc cgctctcgca ccgggctcgg tcttttcgcc
      781 accaagccga tcaagaaggg gacccggatc atccgctatt tcggccctct gctggatgcg
      841 cggaaggacg aggacgttgt cgagaataag tatctgttcg cgctgaacag ccgctggacg
      901 atcgacggct ccgtgcgcag gaatatcgcc cgctacatca atcattcctg caagccgaac
      961 gccgaatccg tcgtcaagcc gcgcaagcgc cgggttttca tccacgccat caagaacatc
     1021 gagaaggacg aggagatcaa ttacgattac ggcgccgaat atttcaaaac gtatctgaag
     1081 ccgatcggct gccgttgcgc ggcatgcgaa gagagcgtcc aggcgcaggc gaaacgggcg
     1141 cggttgaagt caagccgaga aaagaatggc cggacgaagt ctcgaaagcc ggcgaagaaa
     1201 gcggccggag cgcgagcgcg gccgcggaac gggtcgcgct gaacgctcgc cgcggttcag
     1261 aggcgccgac ggcgggaagt ccttcgccgt cgctcatggc gcgggcctgg acgcgcctac
     1321 gccggctccc tctcctcaat ggaggaaaaa gatcgctcag gagcgcggcg ccggctcgtg
     1381 ctcctctttc ggatggcgaa tccagcgccg atggcgacgc caccagcgcc aatgatgatg
     1441 cgggcgccac gagcgccaat aatgatgacg ggcggggcga tgcggcgcgg cgcgatgggc
     1501 cgcggaggtt gcaggttgcg ccgccgtgtt ggacgccggc gcggacttcg ccgcggcggg
     1561 cgccgccggc gcctgatttt gctcattctg gccggcgttg gaggatggag ccggggcgtg
     1621 aggctgcgcc ggcgtttgcg cgctgggggt tggcgcgggc gtcggcggat tggccggcgg
     1681 cggcgtcgat gtcgggtttt gagcgctggg ggtcggagcc ggcgtcggcg tcgccggcgt
     1741 cgaattggcc ggctgcgccg cgggcggagg attctgagcg ctcggcgcgg gattggcggg
     1801 cggcggattg gtcgtcgcgg gctgcgcggg agctgagctc ggcgcgttgg atgtgtcgtt
     1861 gtcggccgcg agcgccggaa gagccgccag actcgccgcc aagaccgccg cggaaaccgt
     1921 tctgatcatc gccgatcccc gaattgtttc aaacgaaacg cctggggcgc ggcgaagttc
     1981 cacaagtccg ctcagcgcat catgtcggcg agcgcgttgc gcatcgccgt gctcggtatg
     2041 ccgaacaatc cgccataggg cagatcgaga tcgaggatga cgaagacggc gaccgtgacc
     2101 gacagggcgc aaagcgcgat gacgatggcg ctcatccggt tcggcggcgc tctcaggccg
     2161 aagctcgcga agagaataac cagccagaag acgagcaccc agtagaacgg cgacgagatc
     2221 gagccgcgca ggccctcgat caccgcccag cggcgccgca acaattccgc atattccccc
     2281 gcgcaagcgg ccataaggtt gtgatgcagc gtgtcggtcg gctcaagcgc gtaaagctcg
     2341 cgcccgatat cgtccatcaa gtcggccagg accggggtct cgccgattcg aggaagatga
     2401 gcgacatccg ggtacgtcac gcctttcggc cgcggctccg aaggccaggt gctggcgatg
     2461 acggcggcga catagctgcg aagctgctcg cgcattttct gcgtttccgg gccgtagtcg
     2521 cgcaggcagc gttcgaactg cgcgagttgc ccggcatagg cgccgcgagc ggcgtaagcc
     2581 atgtcgtagc cgcttttcac ggaggtcgtc aaaagaccca gcacgatcgc ggtgaaggtc
     2641 accaggaggg tgattccgag ctggaccagt tcaatcgtgt cgcgcgagcg atgcttctcc
     2701 gtcaggcggg aattgacgaa acttcccacc gcggcggcga cgcacagcaa aatgagcaaa
     2761 agaagcgagg agtccaaatg ccgcatgtcg ccccctcagg cccaccgcgc gccttcgccg
     2821 ccgacggagg caaaaccgat tttagagcga agaacagcga gtcccgcaag ggaggacgcg
     2881 ttagcgccct cgcctggagc ggcgctcggc gcgcaaaacg cgagaaaagt cgccttcacg
     2941 gcgatttgac gggcctgcgc ttgctcgttt ctgaatcgcg cctaggaatc agcctgaacc
     3001 ggcatatttt cgaaccgttc ggcgcgcgat gagccttccg cgttggttga ccgccgcgcg
     3061 gtttatttgc gtcgccgaca agtcagcaat gcggcggtcg cgggttgccg agcgcgccgc
     3121 gctatggcaa gagccgaccg cttagatcca cgccaagatc aggatgcgat gcctaatttt
     3181 tttggtcaaa acttcaaaga tcgtcagacc gccgccgccg aagccaagaa agccctagcc
     3241 gcgaaattcc tcagtaagcc gcccttcaac cccgaagacc ccgcttacgt cgaacgtcag
     3301 aaacagcgcc gcgccattat cgaggcgcgc gaggagcgcg aggcgcagcg ggcggccgag
     3361 cgcgccgcca aagctgcggc ggaagcggcg cggcgcgccg aagaggagcg ccggcgcgaa
     3421 gccgagcgcc tggagcagga gcggctcgcc gccgaagcgc gcaaggccga agaggagctg
     3481 cgcgagctgc agcgccagga agaggccaag cgcaaggccg agatcgaggc gcagaagaaa
     3541 gccgagcgcg atgcgcgcta cgcggctcgc aaagcccgca aggcggcgcg caaggccgag
     3601 ctcaagcgct actactaaag tccgtagacg cggcggccgg ccgcatcagc cgcgctcatc
     3661 gagcgcggct ccattttagc ccctgacaag cgcctacttc gtcgcctgga tgctgtcgga
     3721 aatcgccttc agatcggcgc cattggcctt ctcgccgtcc ggcgagcccc aataatagag
     3781 cagcaggaat ttgccggcgg cgttggtcgc gaccagcgtc aggctgatat tggcggggcc
     3841 gtctttgtcc ttgccggtaa agttgatgtc gaaggcgtcg agactgttga tcttgatttg
     3901 tttcgtcttc atcgaattgg cgtcgatgtc gacgccgctc ttggcgaaaa atttaattcc
     3961 ttcttcggtc gccttcttca cgtcgtccgc ggcgacttgc tcgatggcga catagattgc
     4021 gccatcatcc gaggttgctt cgaccccgcc ttcgtaaggc tctggattcc atgaatccgg
     4081 aagcgagacc gtggcgatcg ggtcgtccgc gggcacaggg aaggtcttgg ccattgcaag
     4141 gcccggcagc aaggcgaggg cgcccaaggc gatcagggcg aaacggatca tagcggggtt
     4201 ctcccgtgaa aaggcttggt agccgcgagg cgcggcggcc agatattggc ggaatcaccg
     4261 caggagtcga gccccggcgt tcaccgccgc cggctttgtc acgccgccgc cgcgaggcgc
     4321 gcgaaaccgc gatcgagatc gtctttcaag tcctcggcgt cctcca
//
LOCUS       DATGJW010000370         3711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_796992, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000370 DATGJW010000000
VERSION     DATGJW010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3711
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_796992"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..235)
                     /locus_tag="VKU03_11235"
     CDS             complement(<1..235)
                     /locus_tag="VKU03_11235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008039137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatidylserine decarboxylase family protein"
                     /protein_id="HLJ71882.1"
                     /translation="MSVLASIRRQFAPIHPEGYVFIAVFFVATVALDWLWTPLGWIAA
                     VLTLWCAYFFRDPQRVTPWRDGLVVAPADGLISF"
     gene            complement(335..2248)
                     /locus_tag="VKU03_11240"
     CDS             complement(335..2248)
                     /locus_tag="VKU03_11240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400050.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein/permease"
                     /protein_id="HLJ71883.1"
                     /translation="MLQFKTSNFQPAPRPGATLASTARRLWPYIWPKDRADLKLRVVL
                     AVALMIAAKVVTIAVPYAFKWATDALVGETAIDKIPLPKMLTGVVALTVAYGVLRVLM
                     SLFTQGRDALFAAVAMNAVRRLAVEVFVHLHSLSLRFHLERKTGGLTRVLERGRAAIE
                     TIIRTSMLTAVPTIVEFVFVIAAFLISFDWRYVVAVSAMIAAYMTYTTIATNWRIAIR
                     RSMNESDTDANTKAIDSLLNYETVKYFGAEAREAARYDKSMARYERMSVKTYVSLAVL
                     NAGQAIIFTAGLVVAILMCVSGIRSGHNTIGDFALINLMMLQLYQPLNFMGMVYREVR
                     QAIVDIEAMFGILAEHAEIQDAPGAKPLQTPKGAVRFEDVHFNYDPERKILRGVSFEA
                     PAGHTIAIVGPSGAGKSTISRLLFRFYEPSSGRITIDGQDISLVTQKSLRAAIGTVPQ
                     DTVLFNDTIAYNIGYGRDGAGQRDVSQAAASAQIDAFIRSLPQGYDAQVGERGLKLSG
                     GEKQRVAIARTILKGPPILVLDEATSALDSFTEREIQAALERVSKGRTTLIIAHRLST
                     VIHADEILVLDKGVIAERGRHEELLAKGGLYAALWSRQREVDAAEETLRRAGEIAAAD
                     EDLAFTASAALPG"
     gene            2637..3044
                     /locus_tag="VKU03_11245"
     CDS             2637..3044
                     /locus_tag="VKU03_11245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MucR family transcriptional regulator"
                     /protein_id="HLJ71884.1"
                     /translation="MTETLNYIELAAEIVSAYVSNNSVPASDLPGLLSDVHTALVRVS
                     TGAAVAAPETAKPAVPPKKSITNDYIVCLEDGRKFKSLKRHLRTQYNMSPEEYREKWN
                     LPPDYPMVAPAYAKARSALAKQMGLGQQRRRRK"
     gene            3114..3704
                     /locus_tag="VKU03_11250"
     CDS             3114..3704
                     /locus_tag="VKU03_11250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HLJ71885.1"
                     /translation="MATIRNVCVYCGSSAGADPRFGDAAEALGRALATAGIGLVFGGG
                     EEGLMGRVAHAALAAGGRVVGVIPNFLIRKEHALKEAQELIVVDSMHHRKQLMFERAD
                     AFVALPGGVGTLEELVEQLTWTQLLRHAKPVLIADLFGFWRPLLSLFAHMREQGFIRS
                     GFELRYLVAERIEEIVPMLAAAAARRGPETTSFSEA"
BASE COUNT          688 a         1157 c         1207 g          659 t
ORIGIN      
        1 caaagctgat caagccgtcg gccggagcaa cgaccaaacc gtcccgccag ggcgtgacgc
       61 gctgcggatc gcggaagaaa taggcgcacc agagcgtcaa gaccgccgcg atccagccga
      121 gcggcgtcca cagccaatcg agcgccaccg tcgccacgaa aaacacggcg atgaagacgt
      181 agccttcggg gtgaatcggc gcgaattgcc ggcgaatcga agcaagaacg gacatcaggg
      241 tctgttgtcg gaatgtcgcg gtctttttgg cgccaatcgc cgcgaaagtc gaatgagcga
      301 tgcggcgcgc cgcgccgttc atcaagcgtt tcgctcatcc cggcaatgcg gcgcttgccg
      361 tgaaggcgag gtcttcgtca gccgccgcga tctcgccggc gcggcgcaat gtctcctcgg
      421 ccgcgtcgac ttcgcgctgg cggctccaca acgccgcgta gagaccgccc ttggcgagca
      481 attcctcatg acggccgcgc tcggcgatga cgcctttgtc gaggaccagg atttcgtcgg
      541 cgtgaatcac cgtcgacaag cgatgggcga tgatcaacgt ggtgcgtccc ttggacaccc
      601 gctcgagcgc ggcttgaatt tcgcgctcgg tgaaactgtc gagcgccgaa gtcgcctcgt
      661 cgaggaccag gatcggcggc cctttcagga tcgttcgggc gatggcgacg cgctgcttct
      721 cgccgcccga taatttcagg cctcgctcgc cgacctgcgc gtcgtagccc tgcggcaggg
      781 agcgaatgaa cgcgtcgatc tgcgcgctgg cggcggcctg ggatacgtcc cgttggccgg
      841 cgccgtcgcg accgtaaccg atattgtagg cgatcgtatc gttgaagagc acggtgtcct
      901 gcggcaccgt gccgatcgcc gcgcgcagcg atttctgggt gacgagcgag atatcctggc
      961 cgtcgatcgt gatccggccg gacgagggtt cgtaaaagcg gaacagaagg cgcgagatcg
     1021 ttgacttgcc cgcgcccgac gggccgacga tcgcgatcgt atggccggcc ggcgcctcga
     1081 acgagacgcc gcgcaggatt ttccgctccg gatcgtagtt gaaatgcacg tcctcgaacc
     1141 gcaccgcgcc tttcggcgtc tgcaggggtt tggctcccgg cgcgtcctgg atctcggcat
     1201 gctcggccag gatgccgaac atcgcttcga tgtcgacgat cgcttggcgc acttcgcggt
     1261 agaccatgcc catgaaattc agcggctggt agagctgcaa catcatcaga ttgatcagcg
     1321 cgaaatcgcc gatcgtgttg tggcccgagc ggattccgga aacgcacatg agaatggcga
     1381 cgaccaggcc ggcggtgaag atgatcgcct ggccggcatt gagcacggcg agcgagacat
     1441 aggtcttcac gctcatccgt tcgtagcgcg ccatggactt gtcgtagcgg gccgcctccc
     1501 tcgcctcggc gccgaaatat ttcacggtct cgtaattaag caggctgtcg atcgctttgg
     1561 tgttggcgtc ggtatcgctt tcgttcatcg agcggcggat ggcgatccgc cagttggtcg
     1621 cgatcgtggt gtaggtcata taggcggcga tcatcgccga gaccgcgacc acgtagcgcc
     1681 agtcgaacga gatcaggaag gcggcgatga cgaagacgaa ctcgacgatg gtcgggaccg
     1741 cggtcagcat cgaggtccgg atgatcgtct cgatcgcggc gcggccgcgc tcaagcacgc
     1801 gggtgaggcc gccggtcttg cgctccaaat gaaagcgcaa ggagagcgaa tggagatgga
     1861 cgaagacctc gacggcgaga cggcgcaccg cgttcatcgc cacggcggcg aacagcgcgt
     1921 cgcgcccttg ggtgaacagc gacatcagca ccctgaggac cccataggcg accgtcaagg
     1981 cgacaacgcc agtcagcatt ttcggcagcg ggattttgtc gattgcggtc tcgccgacca
     2041 aagcgtcggt cgcccatttg aaggcgtagg gaacggcgat cgtcacgact ttggcggcga
     2101 tcatcagcgc gaccgccaag accacccgca atttcaaatc cgcccggtcc ttgggccaaa
     2161 tatagggcca caatcgccgg gcggtcgacg ccagcgtcgc gccggggcga ggcgcgggct
     2221 ggaaattcga ggtcttgaat tgcagcattt cggctcgcgg ctgaggcttc gatccggcaa
     2281 tatagggcgc tcgccgcctc gccgcatccc catcgtcccg ctttgcagag gcaaatcgcg
     2341 accggcgaca atattgattc ggcggcgggc cgaaatctcg gcgaggcgac gtgtactcaa
     2401 gtaagcgccg gccggattgc gacccccaag gaaagatcaa gcgccgtcaa aaagaatata
     2461 aaagctccgc tatattgttc ttgccgcggc cattccttac gcctatagat tccttcgcga
     2521 ccgtcccgcg cgactcgtcg tcaagcctag gtcgagaccg ctcaatgagg ggcaaagcgc
     2581 aatcttgccg taagctcttt cgcgcgccgc aagattagcg gtggagcaac aacgacatga
     2641 ccgagacgtt gaattatatt gaactcgcgg ctgaaatagt ttccgcctat gtgtctaata
     2701 attcggtgcc ggcaagcgat cttccagggc tgctgagcga cgttcatacc gccttggtgc
     2761 gcgtctccac tggcgcggcg gttgcggcgc cggaaaccgc caagccggcc gtgccgccga
     2821 aaaagtcgat caccaacgac tatatcgttt gcctcgagga cggccgcaaa ttcaagtcgc
     2881 tgaagcggca tttgcgcacg caatacaaca tgtcgccgga agaatatcgc gagaaatgga
     2941 atcttccgcc cgactatcca atggtcgcgc cggcctacgc caaggcgcgc tcggccttgg
     3001 ccaagcagat ggggctcggc cagcagcgcc gccgccgcaa atagtcgcca cgctcaagcc
     3061 ggcggcgcgc cgcgcgcgct tgacctttgg cggcatggcg cgccactagg cccatggcga
     3121 cgattcgaaa cgtctgcgtt tattgcggct cgagcgccgg cgccgatccg cgcttcggcg
     3181 atgcggcgga ggcgctcggg cgcgctcttg cgaccgccgg gatcgggctg gtctttggcg
     3241 gcggcgaaga ggggctgatg ggccgcgtcg cgcatgccgc tctggccgcc ggcggccgcg
     3301 tcgtcggggt tattccgaac ttcctgatcc gcaaggagca tgcgctcaaa gaagcgcagg
     3361 agctgatcgt cgtcgacagc atgcatcacc gcaagcagtt gatgttcgag cgcgccgacg
     3421 cgttcgtggc gctgccgggc ggcgtcggca cgctggagga gctggtcgag cagctcacct
     3481 ggacgcaatt gctccgccac gccaagccgg tcctgatcgc cgatctcttc gggttctggc
     3541 gtccgcttct ctcgctcttc gcccatatgc gcgagcaggg cttcattcga agcggcttcg
     3601 agctgcgcta tcttgtcgcg gagagaatcg aggagatcgt gccgatgctc gccgccgccg
     3661 cggcgcgccg ggggccggag acgacctcgt tttccgaggc ttgaccggcg g
//
LOCUS       DATGJW010000371         6102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_587807, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000371 DATGJW010000000
VERSION     DATGJW010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6102
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_587807"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1232
                     /locus_tag="VKU03_11255"
     CDS             <1..1232
                     /locus_tag="VKU03_11255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HLJ71886.1"
                     /translation="WFAGDGMLHAFTLREGRASYRNRWVRTLKWLAEHDAGRALFSGW
                     GVLKPDAPPSTARDAGAANTNIVWHGGRLLALEEAHRPTEIDPLSLATRGYCDFNGAV
                     QGPFTAHPKIDPKTGEMIFFGYNAKGSFSNVMSYGAIGRDGVVTRFEHFETPYASMAH
                     DFIVTDNHVLFPILPLTGSMERARRGAPPYAWEPDKGSYVGLLKRGAPADAIRWFRGE
                     ACYVFHVLNAWEEGGRIVADVMQFEEPPLFPRPDGTPTDKKKSRARLCRWTFDLAAKT
                     DTFTREYLDDLRGEFPRIDERRAGLANRHGWFACASAAGEERGFFDGVAHISGGERKT
                     YFLPAGDATSEAVFAPRAADAAEGDGWLLAVIWRGAENRSDLAIFNATEIERGPIALV
                     ELPHRVPFGFHGNWVSA"
     gene            1242..1805
                     /locus_tag="VKU03_11260"
     CDS             1242..1805
                     /locus_tag="VKU03_11260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71887.1"
                     /translation="MQIVLGFAPFIAFALLTRFVGVEASLSIAAAIAAGLAIRNRFAG
                     RSIKILEAGTVALFGLLALHGALTQSTWSLPLVRVVVDAGLLAIVLLSIAIGVPFTLQ
                     YAREQVPPEIQASPVFLRVNKAISAVWALAFAIGLIADLAMEYAPSAPLWLDIAVIVA
                     ALAGAARFTQWYPEKVRRSFGGGARAA"
     gene            complement(1808..2320)
                     /locus_tag="VKU03_11265"
     CDS             complement(1808..2320)
                     /locus_tag="VKU03_11265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein tyrosine phosphatase"
                     /protein_id="HLJ71888.1"
                     /translation="MSFIHVCSLARIEAAAAETGARSMVTLLQAGTAMTRPAAIAVDR
                     HLFLALSDITEPMDGHVLPGEEHVRTLLDFARSWDRIAPMLIHCFAGVSRSTAAAFIS
                     ACALNPDRKERDIARAIRAASPTATPNARLVAVADAILGRRGRMNEAIAEIGRGEACS
                     EGVPFSLAIA"
     gene            complement(2385..2996)
                     /locus_tag="VKU03_11270"
     CDS             complement(2385..2996)
                     /locus_tag="VKU03_11270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD family hydrolase"
                     /protein_id="HLJ71889.1"
                     /translation="MAAERQRAWQRMLSGRRLDLIDPSPLDIEIEDIAHGLARVARWN
                     GQTYGPEIFSVAQHSLLVEALIGGLAPQASRIWRLAALLHDAPEYVIGDMISPFKAVV
                     GGDYRAVEQRLLAATFLRFGLPPQPPLELTSLIKTADRAAAYLEATRVAGFSTAEAKR
                     LFDPPRIATKSVERYLAPLSPALAEESFLLRFAELDQGARAAS"
     gene            complement(3025..3657)
                     /locus_tag="VKU03_11275"
     CDS             complement(3025..3657)
                     /locus_tag="VKU03_11275"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011501659.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HLJ71890.1"
                     /translation="MAEKSKPPRPTASVLHGDGLCRCPWPGHDPLYVAYHDEEWGVPE
                     WDDRALFEKLILDGFQAGLSWITILRKREAFRAAFDGFDPQKIVRYRPAKIEKLMNDP
                     GIVRNRAKIEATMALARIYLDLAEQGGFARHLWSFVGGKPIQQARKSTRDVPAQSPEA
                     EAIAKDLKARGGNFVGPTIVYAFMQATGMVNDHLVDCHRHQACAALAAGR"
     gene            3815..4072
                     /locus_tag="VKU03_11280"
     CDS             3815..4072
                     /locus_tag="VKU03_11280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71891.1"
                     /translation="MFASHPRTLDELSAADARRAALAYVSEAFAEAVLDGIDVDLFAE
                     AALCAAFNELVAVHGEEKAAAFAERLAERVRQGEFSAVARQ"
     gene            4174..5157
                     /locus_tag="VKU03_11285"
     CDS             4174..5157
                     /locus_tag="VKU03_11285"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013419543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004024 - alcohol dehydrogenase activity,
                     zinc-dependent [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0030554 - adenyl nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MDR family oxidoreductase"
                     /protein_id="HLJ71892.1"
                     /translation="MTFKALRVDKSGQATDVRFVEMSDDELMDGDVTLRVTHSTVNYK
                     DALVMAGRPGIVQRFPMIAGIDLAGIVETSRNSNFQPGDEALLNGYGLSQTHFGGYAE
                     KARVPGEWLIKLPPGMTRAETMAIGTAGYTAMLCVLALERQGVKPEQGPALVTGAAGG
                     VGSVAVALLAAAGWRVEASTGRANEAGYLRDLGAAEIIDRGELSAPGKPLGKERWAAA
                     IDSVGSHTLANAIAGLKRGGAAAACGLAQGMDLSMTVAPFILRGVQLIGIDSVWCPME
                     RRLEAWRRLARDLDRKKLAGMTKTVPFERVMETGANLLAGKVRGRVVVEIG"
     gene            5205..5933
                     /locus_tag="VKU03_11290"
     CDS             5205..5933
                     /locus_tag="VKU03_11290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HLJ71893.1"
                     /translation="MTDVTFPAAAAAGLISFLSPCVLPLAPPYLCYLAGASIEEVAVD
                     GEARARRTILITAALFVLGFATVFIALGATASVFGALLRQWSRELSIAAGIGIAIMGL
                     HFLGLFRIGALFREKRLEIERPPGLWSAYLMGLAFAFGWTPCIGPILAAILAVAGSED
                     TVGRGAALLAAYSLGLGAPFLVAAFAMGPFLRAARRFRSQFGRVEKLVGALLVLTGVA
                     FLSGGVQAASQWLIDAFPALSQLG"
BASE COUNT          957 a         1992 c         2057 g         1096 t
ORIGIN      
        1 attggttcgc cggcgacggc atgctgcacg cattcacttt gcgggaaggc cgcgcgagct
       61 accgcaaccg ctgggtgcgc accctgaaat ggctcgccga gcatgacgcc ggccgggcgc
      121 tgttttccgg ttggggcgtt ctcaagccgg acgcgccgcc ctcgaccgcc agggacgcgg
      181 gcgccgccaa caccaatatc gtctggcatg gcggccggct gctcgccttg gaggaggcgc
      241 atcggccgac cgagatcgat cccctcagct tggcgacgcg cggctattgc gatttcaacg
      301 gcgcggttca ggggcctttc accgcccatc ccaagatcga tccgaagacc ggcgagatga
      361 tcttcttcgg ctataacgcc aagggatcgt tctccaacgt catgtcctat ggcgcgatcg
      421 gccgcgacgg agtcgtgacg cgcttcgagc atttcgagac gccctacgcc agcatggcgc
      481 atgatttcat cgtgacggac aatcatgtcc tgtttccgat tctgcctttg accggcagca
      541 tggagcgcgc gcggcgcggc gcgccgccct atgcctggga gccggacaag ggctcttatg
      601 tcggcctgct caagcgcggc gcgccggccg acgccatacg ctggttccgt ggcgaggctt
      661 gctatgtctt ccacgtgctc aacgcctggg aggagggcgg ccggatcgtc gcggacgtga
      721 tgcaattcga ggagccgccg ctttttcctc gtcccgacgg aacgccgacg gataagaaaa
      781 aatcgcgggc gcggctgtgc cgctggacgt tcgatctcgc cgccaagacg gacacgttca
      841 cgcgcgaata tctggacgat ttgcgcggcg agtttccgcg catcgacgag cgaagggccg
      901 ggctcgccaa tcgccacggc tggtttgctt gcgcgagcgc ggcgggcgag gagcgcggct
      961 tttttgacgg cgtcgcccac atatcgggcg gcgagcgcaa gacctatttc ctgccggcgg
     1021 gcgatgcgac gtcggaagcg gtgttcgcgc cgcgcgccgc cgatgcggcg gagggcgacg
     1081 gctggcttct cgccgtcatc tggcgcggcg cggaaaaccg cagcgatctc gcgattttca
     1141 acgcaaccga aatcgagcgc gggccgatcg ccctggtcga attgccgcac cgggttccgt
     1201 tcggctttca cggcaattgg gtcagcgcct aggaggaaag gatgcaaatt gtcctcggct
     1261 tcgcgccgtt catcgctttt gcgctattga cccgcttcgt cggcgtcgag gcgagcctgt
     1321 cgatcgcggc ggcgatcgcc gccgggctgg cgatccgcaa tcgcttcgcc ggccgctcga
     1381 ttaaaattct cgaagcggga acggtcgcgc tgttcggcct gctcgcgctt cacggcgcgc
     1441 tgacgcaatc gacctggagc ctgccgctgg tgcgcgtcgt cgtcgatgcc ggcctcctcg
     1501 ccatcgtcct tctgtcgatc gccatcggcg tacccttcac cctgcagtat gcgcgcgagc
     1561 aggttccgcc ggaaatccag gcgtcgcccg tgttcctgcg ggtgaacaag gcgatcagcg
     1621 cggtctgggc gcttgctttc gccattggcc tgatcgccga tctggcgatg gaatatgcgc
     1681 caagcgcgcc gctttggctc gatatcgccg tcatcgtcgc cgcgctggcc ggcgcggcgc
     1741 gcttcacgca atggtacccc gaaaaggtgc gccgctcttt cggcggcggc gcgcgcgccg
     1801 cttaagatca ggcgatggcg agcgaaaagg gaacgccttc gctgcacgct tcgccgcggc
     1861 cgatctcggc gatcgcctca ttcattcggc cgcgccggcc gagaatcgca tcggccacgg
     1921 cgacgaggcg ggcgttgggc gtggcggtcg gcgaagcggc gcggatcgcg cgagctatgt
     1981 ccctctcctt gcggtctgga ttgagggcgc aggccgaaat gaacgccgcc gccgtcgagc
     2041 ggctgacgcc ggcaaaacaa tggatcagca tcggcgcgat gcgatcccag gagcgggcga
     2101 agtcgagaag cgtacgcaca tgctcttcgc caggcagaac atggccgtcc atcggctcgg
     2161 taatgtccga cagcgccagg aagaggtggc ggtcgaccgc gatcgccgcc gggcgggtca
     2221 tcgccgtgcc ggcttgcaac aaagtgacca tcgagcgcgc gccggtttcc gccgccgccg
     2281 cctcgatccg cgccaaagag cagacgtgaa taaaggacat cgtttcctgt tccccttaga
     2341 gaggcaggat taattccttt cgcggcctta tgcaaagccg gccgtcacga agcggcgcgg
     2401 gcgccctggt caagctcggc gaagcgcagc aggaagctct cctcggccaa agcgggactg
     2461 agcggcgcga gatagcgctc gacgcttttc gtcgcgattc gcggcggatc gaacaggcgc
     2521 ttggcctcgg cggtcgaaaa gccggcgacg cgcgtcgctt cgagataggc ggcggcgcga
     2581 tcggcggtct tgatcagcga ggtcaattcc agcggcggct gcggcggcag gccgaaacgc
     2641 aggaaggtgg cggcgagcag acgctgctcg acggcgcgat aatcgccgcc gaccaccgcc
     2701 ttgaacggcg agatcatgtc gccgatcacg tattccggcg cgtcatgcag caaagcggcg
     2761 agtcgccaaa tcctggatgc ttgcggcgcg agcccgccga tcagcgcctc gacgagcagc
     2821 gaatgctggg cgacggagaa gatttccggc ccgtaggtct ggccgttcca gcgcgcgacg
     2881 cgcgccaagc catgggcaat atcctcgatc tcgatgtcga gcggcgacgg atcgatgagg
     2941 tcgagccgcc gtccggagag catcctctgc catgcgcgct gtcgctcggc ggccatgaaa
     3001 gctccgatcg tccttcggcc gcgctcagcg ccccgccgcg agcgcggcgc aagcctgatg
     3061 gcgatgacaa tcgacgagat gatcgttgac catgcccgtc gcctgcatga aggcgtagac
     3121 gatggtcggc ccgacgaaat tgccgccgcg cgctttgagg tccttggcga tggcttccgc
     3181 ctccggcgat tgcgccggca cgtcgcgggt cgattttcgc gcctgctgga tcggcttgcc
     3241 gccgacgaag ctccataagt ggcgggcgaa gccgccctgt tcggcaagat cgagatagat
     3301 gcgggcgagc gccattgtgg cctcgatctt ggcccggttg cgtacgatgc cgggatcgtt
     3361 catcagcttt tcgattttcg ccgggcggta gcgtacgatc ttctgcggat cgaatccgtc
     3421 gaaggcggcg cgaaacgctt cgcgcttgcg caggatcgtg atccaggaaa ggccggcttg
     3481 aaagccgtcg agaatcagtt tttcgaagag cgcgcggtcg tcccattcgg gcacgcccca
     3541 ttcctcgtcg tgataggcga cgtaaagcgg gtcgtggccc ggccaggggc agcggcagag
     3601 cccgtcgcca tgcaggacgg aggcggtcgg gcggggcggc tttgattttt cggccaaggg
     3661 cgtctccttc ggaggcgcaa gatcgcctac caaagcaggt ccggcaagcg gttgcgccgg
     3721 acgacggctt cgcgggcctt gcgccggtcg tgttaacctt tcctcaagac tcagcttcgc
     3781 cagacacggc gcttgccgat aaaatgcccg cgcaatgttc gcgtcacatc ctcgcaccct
     3841 cgacgaactg tcggcggccg acgcccgccg cgcggcgctc gcctatgtca gcgaagcctt
     3901 cgccgaggcc gtactcgacg gcatcgatgt cgatctcttc gccgaagccg cgctttgcgc
     3961 cgccttcaac gaactcgtcg ccgtgcatgg cgaggaaaaa gccgccgctt tcgccgagcg
     4021 gctggccgag cgggttcggc agggcgaatt ttccgccgtc gcccgtcagt aaggcttcaa
     4081 ttttgtcgca agccgcgcga taacccgtct ggatcggcgc ggccataagc ccgcctgtcg
     4141 gagggcttgg gcgacagccc ggcgcggagc ggcatgactt tcaaagcctt gcgggtcgac
     4201 aaaagcggcc aggcgacgga tgtgcgcttc gtcgagatga gcgacgacga actgatggac
     4261 ggcgacgtca cgctccgggt gacgcattcg acggtcaatt acaaggacgc gctggtcatg
     4321 gccggacggc ccggcatcgt ccagcgcttt ccgatgatcg ccggcatcga ccttgccggg
     4381 atcgtcgaaa cctcccgcaa ctccaatttt cagccaggcg acgaggcgtt gctcaacggc
     4441 tatggcctga gccagacgca ttttggcggt tatgccgaaa aagcgcgcgt ccccggcgaa
     4501 tggctgatca agctgccgcc cggcatgact cgcgccgaaa ctatggcgat cggcacggca
     4561 ggctatacgg caatgctctg cgtgctggcg ctggagcgtc agggcgtgaa gccggagcag
     4621 gggccggcgc tggtgaccgg cgcggccggc ggcgtcggct ccgtggcggt cgcgcttctg
     4681 gctgcagccg gttggcgggt tgaagcctcc acggggcgcg ccaacgaagc cggctatttg
     4741 cgcgatcttg gcgcggcgga gattatcgac cgaggcgagc tttccgcgcc gggcaagccg
     4801 ctcggcaagg agcgctgggc cgccgcgatc gacagcgtcg ggtctcacac gctcgccaat
     4861 gcgatcgccg gcctcaagcg cggcggcgcg gccgcggcct gcggcctcgc ccagggcatg
     4921 gacctttcca tgactgtcgc gccgttcatc ttgcgcggcg tccagctcat cggaatcgac
     4981 agcgtctggt gtcccatgga gcgccggctc gaagcttggc ggcggctcgc gcgcgatctg
     5041 gaccgcaaga agctcgccgg gatgacgaag accgttccgt tcgagcgcgt catggaaacc
     5101 ggcgccaatc ttctcgccgg caaggtgcgc ggccgcgtgg tggtcgaaat tggctgaacg
     5161 gattgtgaat tgccgttggg cgcagcggcg gcgatgaaca ggcgatgacc gacgtaacct
     5221 ttccggctgc cgcggcggcg ggcttgattt cattcctcag cccctgcgtc ttgcccctgg
     5281 cgccgcccta tctctgctat ctcgccggcg cgagcatcga agaagtggcc gtcgacgggg
     5341 aggcgcgggc gcgccggacg atcctgatca ccgcggcctt attcgtcctc ggcttcgcga
     5401 ccgtattcat cgcgctcggc gcgaccgctt cggtcttcgg cgcgttgctc cggcaatgga
     5461 gccgggagct ttcgatcgcg gccggaatcg gcatcgcgat tatgggcctg cactttctcg
     5521 gcctcttccg catcggcgcg ctgtttcgcg agaagcggct cgaaatcgag cggccgcccg
     5581 gcctgtggag cgcctatctg atgggcctcg ccttcgcctt cggctggacg ccctgcatcg
     5641 gcccgattct cgcggcgatc ctcgcggtgg cgggatcgga ggatacggtc ggccgcggcg
     5701 ccgctttgct cgccgcctat tcgctcggcc tcggcgcgcc gttccttgtc gccgccttcg
     5761 cgatgggccc gttcctgcgc gccgcgcgcc gctttcgcag ccaattcggc cgggtcgaga
     5821 agctggtcgg cgctctgctg gttctgaccg gcgtcgcctt tctgagcggc ggcgtccagg
     5881 ccgcttcgca atggctgatc gacgcctttc cggcgctgag ccaactcggc tgaagccgtc
     5941 attgcgaccg cgcctacaag ccgaacgtct cacaagaaaa gagcccgccg tcgccggcgg
     6001 gctctcgttt tccaggccgg atcgactgat cacatctgtt tataggtgat cttgccgtcg
     6061 tcgcccttct tccagacgta ccagacatag tcgatcgtcg tg
//
LOCUS       DATGJW010000372         2644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1001845, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000372 DATGJW010000000
VERSION     DATGJW010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2644
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1001845"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..>2644
                     /locus_tag="VKU03_11295"
     CDS             32..>2644
                     /locus_tag="VKU03_11295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406487.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HLJ71894.1"
                     /translation="MTFAAPPPGLISQVLEDASEIQAILGPTNTGKTHYAIERMLAHR
                     SGVIGLPLRLLAREVYARVAERAGAASVALITGEERINPGDPRYWVCTVEAMPRGLRP
                     DFLAVDEIQLAADLQRGHAFTDRLLNYRGRVETLFLGAETIQPVIGALAPRATLTARP
                     RLSSLIHAGERKLTRLPRRSAIVAFSAQDVYEIAELIRRRHGGAAVVLGALSPRTRNA
                     QVDLYQNGDVDYIVATDAIGMGLNLDIEHVAFASDQKFDGREYRRLTHAEFAQIAGRA
                     GRNRRDGAFGTTGRCPPFEPELVEALESHIFAPSARALWRNSELDFSSIDALLQALRR
                     FPDRPGLMRSDSGDDIRALEILANDAAVRRSAKTSADIARLWDACQIPDYRNVSGGAH
                     AELVKSVFEFIVRKGKVSEDWFWRHLSSADKAEGDLDALSSRIDQVRSCAYMANREDW
                     LADPGHWQVIARQVEDRLSDALHERLAQRFVDRRTSFLLRRYRENAMLNAEVTAAGDL
                     TVEGQMAGRLQGFRFTPDSKASPEAAKVASQAIAEAIAREGDLRAARLAEAVDSSLVL
                     ANDGAIRWLGDPIAKLAAGDKLLQPRALILADDAVTNGAKEIVQRRVDLWLAAHVKRL
                     LGPLIELEAGEGLDGAARGVAFQLGEALGVLERSRVAPEVKGLDQNARGALRKLGVRF
                     GAYYLYLPALLKPAPRVLALQLWALRNPDAANEDRLAELPQIAGAGRTSFVADKGLSR
                     DILRIAGFRLCGERAVRVDILERLADLIRPAVAYRPGITPGLPPPGAADGDGFVATVA
                     MTSLTGCSGEALASILRSMGFDSRKVKGPAITVPLAAPTTPAAPQEQTAAEEANANAG
                     ASEAQAEAAPVTEQA"
BASE COUNT          416 a          906 c          879 g          443 t
ORIGIN      
        1 aggcgaccgc ttttcaggat ttcgagcgcc catgacattc gccgcgccgc cgcccggcct
       61 tattagccaa gttcttgaag acgcctcaga aattcaagcg attctcggcc cgaccaatac
      121 cggcaagacg cattatgcga tcgagcggat gctggcacac cgctctggcg tcatcggtct
      181 gccgctgcgg ctcctcgcgc gcgaggtcta tgcgcgcgtc gccgagcggg cgggcgcggc
      241 gtccgtcgcg ctgatcacgg gcgaagagcg gatcaatccg ggcgatccgc gctattgggt
      301 ctgcacggtc gaggccatgc cccgcggctt gcggccggac ttcctcgccg tggatgaaat
      361 ccagctcgcc gccgatttgc agcgcggcca cgccttcacc gatcggctgc tcaattaccg
      421 cggccgcgtc gagactctgt tcctcggcgc cgagacgatc cagccggtca tcggcgcgct
      481 cgcgccgcgg gcgacgctga cggcgcgccc gcggctttcc tcgctcattc acgccggcga
      541 gcgaaaattg acgcgtttgc cgcggcgttc ggcgatcgtc gccttttctg cgcaggacgt
      601 ttacgagatc gccgaactca tccgccgccg tcacggcggc gcggcggtgg tgctcggcgc
      661 gctgtcgccg cgcacgcgca acgcgcaggt cgatctctat caaaacggcg acgtcgacta
      721 catcgtcgcc accgacgcga tcggcatggg actcaatctc gatatcgagc acgtcgcctt
      781 cgcttctgat cagaaatttg acggccgcga ataccgacgg ctgacccatg cggaatttgc
      841 ccaaatcgct ggccgggccg gccgcaaccg ccgcgacggc gcgttcggca ccaccggacg
      901 ctgcccgcct ttcgagcccg aattggtcga agcgctcgaa agccacattt ttgcgccctc
      961 ggcgcgcgca ttgtggcgca acagcgagct cgatttctct tcgatcgacg ccttgctgca
     1021 ggcattgcgg cgttttcccg accgacctgg cttgatgcgc tcggattccg gcgacgacat
     1081 cagagccctg gagattcttg ccaatgacgc cgccgtgcgt cgatcagcca agacctcggc
     1141 cgatatcgcc cgcctctggg acgcatgcca aattcccgat taccgcaatg tctcgggcgg
     1201 cgctcacgcc gagctcgtca agtcggtttt tgaattcatc gtgcgcaagg gcaaagtttc
     1261 cgaagactgg ttctggcggc atttgtcgag cgcggacaag gccgaaggcg atcttgacgc
     1321 gttgtcctcg cgaatcgatc aggtccgctc ctgcgcctat atggccaatc gcgaagattg
     1381 gctcgccgat ccgggacatt ggcaggtcat agcgcgtcag gtagaggaca ggctgtcgga
     1441 cgccctccac gagcggctcg ctcagcgatt cgtcgaccgg cgcaccagct ttcttttacg
     1501 ccgctacaga gagaacgcga tgctcaatgc ggaagtgacc gcggccggcg acctgaccgt
     1561 cgaggggcag atggcggggc ggctgcaggg atttcgcttc accccggatt ccaaagcgag
     1621 ccccgaggcc gccaaggtcg cgagccaggc gatcgcggag gcgatcgcgc gggaaggcga
     1681 tttgcgcgcc gcgcggcttg ccgaagcggt cgattcctcg ctcgtcctcg ccaatgacgg
     1741 cgcgatccgc tggctcggcg acccgatcgc caagcttgcc gccggcgata aattgctgca
     1801 gccgcgcgcg ctgatcctcg ccgacgacgc ggtgaccaac ggcgcgaagg agatcgtcca
     1861 gcgccgcgtc gatctgtggc tggccgccca tgtcaagcgg ctgctcgggc cgctgatcga
     1921 gctcgaagcg ggcgaagggc tggacggggc ggcgcgcggc gtcgccttcc agcttggcga
     1981 ggcgcttggc gtgctcgaac gcagccgcgt cgcgccggag gtcaagggcc tcgaccagaa
     2041 cgcgcgcggc gcgctgcgca agctcggcgt ccggttcggc gcctattatc tctatctgcc
     2101 cgcgctgctg aagccggccc cgcgcgtgct cgcgttgcag ctttgggcgc tgaggaaccc
     2161 ggatgcggcg aacgaggacc gtctcgccga attgccgcag atcgccggcg ccggccggac
     2221 ctcgttcgtc gccgacaagg gcctgtcgcg cgacatcctg cgcatcgccg ggttccggct
     2281 ctgcggcgaa agggccgtca gggtcgatat tctcgagcgg ctcgccgatc tcatccggcc
     2341 cgccgtcgcc tatcggccgg ggatcacgcc cggcctgccg ccgcccggcg ccgccgatgg
     2401 cgacggcttc gtcgcgacgg tggcgatgac ctcgctgacc ggatgctccg gcgaagcctt
     2461 ggcgtcgatt ttgcgctcga tgggcttcga cagccgcaaa gtgaaggggc cggcgatcac
     2521 cgtcccgctc gccgcgccga cgacgccggc cgcgccgcaa gaacagacgg cggcggagga
     2581 ggcgaacgcg aacgcggggg cgtcagaggc ccaagcggaa gcggcgcccg tgacggagca
     2641 agcc
//
LOCUS       DATGJW010000373        11864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_900984, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000373 DATGJW010000000
VERSION     DATGJW010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11864
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_900984"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(153..488)
                     /locus_tag="VKU03_11300"
     CDS             complement(153..488)
                     /locus_tag="VKU03_11300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71895.1"
                     /translation="MRGFWLFASALVIAALTSAASAGDHWKFSVVNKSNLAAVEFRTQ
                     EDGEWSANWIKDRIEPGDKFDMDFGTEKGDCTVRTQIHFTDGSYFDADVDYCKVSTLY
                     IYDNKLTWD"
     gene            complement(593..1201)
                     /locus_tag="VKU03_11305"
     CDS             complement(593..1201)
                     /locus_tag="VKU03_11305"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209754.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HLJ71896.1"
                     /translation="MFYETRQRDKALLPHDPFKAIVAPRPIGWISTRAKDGRVNLAPY
                     SFFNGFCSAPPIVGFSSEGAKDSAAFAVESGEFVANLATMDLAQPMNATSAPLARGDN
                     EFLHAGLTMAPCRLVAAPRVAEARAALECKVTEMVEIRNAKAESLDRYLVLGEVVGVH
                     IDDRFVKDGRFDTAAAQPIARCGYHDYAVVERLFSMIRPAGG"
     gene            complement(1284..1850)
                     /locus_tag="VKU03_11310"
     CDS             complement(1284..1850)
                     /locus_tag="VKU03_11310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase"
                     /protein_id="HLJ71897.1"
                     /translation="MTNPTIDLLLSRRSAPPQTLHGPGPNAEEIETLLRIASRVPDHG
                     KLAPWRFLLFEGAARDRAGAIAAELLARDNPQADAAKIAAERGRFARAPLVVAVVSRA
                     APHVKIPEWEQVLSAGAVCMNLQIAANALGYKSIWVTEWCAYDRRFCNALGLAGHEKI
                     AGFIHIGRPSAVIEDRARPTLDEIVTRF"
     gene            2012..2914
                     /locus_tag="VKU03_11315"
     CDS             2012..2914
                     /locus_tag="VKU03_11315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71898.1"
                     /translation="MLRLVQRLALWLLLGAVAAGPAAGQTTIPTTLPGKAVLHLSAIL
                     SGQADPLRGGLHWRLYPARAEADGARALIAESEVAQPTFVVPPGDYVVHLAFGLAGQT
                     KRVTLGEEERSERLTLNAGALKIVGTVGGAVIPSNRLALEIFVPQGHNPEGKLVDAKA
                     SAGELIGLPEGPYHIVSTYLDQGAGPSGGKSANPPIPTNSIVAADIKVPAGKVIDLTL
                     RHRFATLTLKLVNAPGAEALANSSFTVLTPGGDLIRELVGAFPSLVLAEGEYIVIARH
                     DQKTYQTTFQVQSALDRDVEVIAQ"
     gene            2939..3175
                     /locus_tag="VKU03_11320"
     CDS             2939..3175
                     /locus_tag="VKU03_11320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC family transcriptional regulator"
                     /protein_id="HLJ71899.1"
                     /translation="MQTFFVQIKCALGRTYDVANALAEAEIASEIYSTAGDFDILAKF
                     YVERETDIGHFVGQKVQIIPGIVDTRTIITFKAF"
     gene            3237..3638
                     /locus_tag="VKU03_11325"
     CDS             3237..3638
                     /locus_tag="VKU03_11325"
                     /inference="COORDINATES: protein motif:HMM:NF020395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ivy family c-type lysozyme inhibitor"
                     /protein_id="HLJ71900.1"
                     /translation="MRLAAILAFTLVAGQAAAQNGPYLFDVLLKGPYRIGWEKLLKQV
                     QPTPDWLLRFSKDFDGVSGQLVGTAIDGKAYELSFVCEPQHCKARRFEVMFEVSTKRA
                     YGALYFEGEPPAFYGDPTPAMKEEMIKALKG"
     gene            complement(3691..4779)
                     /locus_tag="VKU03_11330"
     CDS             complement(3691..4779)
                     /locus_tag="VKU03_11330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015341609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine-2,3-aminomutase-like protein"
                     /protein_id="HLJ71901.1"
                     /translation="MHESLSSDRAAAPRALKTAADLVAAGLVAEARAAEIARVAERYA
                     VAIPPALAALIDPADPADPIARQFVPDRRELIAHPAELADPIGDELKSPVKGIVHRYP
                     DRALLKLVSVCAVYCRFCFRRETVGPRAAPALSAEEFEAALAYLREHEEIWEVVVTGG
                     DPLVLSPRRLAAATRALAEIGHVKVLRWHTRLPVADPQKVDSAMAEALADAPGKAVYV
                     ALHVNHPRELTDAARAACKRLIGAGAAMVSQTVLLRGVNDNVETLEALMRALVETRIK
                     PYYLHHGDLAPGTAHLRATIADGQALMRQLRGRLSGLAQPTYVLDIPGAHGKAPIGPG
                     YARAAGEGRYEISDPAGGAHFYTDCCSD"
     gene            complement(4963..6759)
                     /locus_tag="VKU03_11335"
     CDS             complement(4963..6759)
                     /locus_tag="VKU03_11335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 15 protein"
                     /protein_id="HLJ71902.1"
                     /translation="MPNRIEDYAVIGNCETAALVGRDGSIDWLGLPRFDSAACFAALL
                     GEPKHGRWLIAPADESRKVARRYRGDTLVLETEFETPDGAVRLCDFMIRREGSSELLR
                     LVQGLSGKVAMRMELIVRFEYGAIVPWVSRQPDGRLQFIAGPDRLLLATKVATRGEDF
                     RTLSEFEIGADDEISFCLNWSPSFRPPPAELDARTALDGAVSRWSQWAATFKPPEQYG
                     DAVLRSLLTLKALAHWETGGIVAAATTSLPEKLNGSRNWDYRYCWLRDATFTLYALIG
                     AGFLDEAKAWREWLVRAVGGSPDNLQIMYGVAGERRVNEYEIPWLPGYGGAKPVRVGN
                     AAVEQLQLDVYGEIVDALYVGRKAGLAPDAASWMLECALVEHLEKIWRQPDNGIWEVR
                     GGPRHFTHSKVMAWVAFDRVVRSIEEFGLDGPLDRWRAIRAAIHKDVCDNGYDPRQNA
                     FVQSYGATALDASLLLIPMVGFLPADDDRVKGTVAAIERNLLRDGFVIRYDTGKKTDG
                     LPPGEGVFLACSFWLADNYILLGRDRDARALFERLLALRNDVGLLAEEYDPSDKRQLG
                     NFPQAFSHLALINTAHNLASLAGPVHQRSGGG"
     gene            complement(6866..6952)
                     /locus_tag="VKU03_11340"
     tRNA            complement(6866..6952)
                     /locus_tag="VKU03_11340"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6916..6918),aa:Leu,seq:taa)
     gene            complement(7470..7973)
                     /gene="gpt"
                     /locus_tag="VKU03_11345"
     CDS             complement(7470..7973)
                     /gene="gpt"
                     /locus_tag="VKU03_11345"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HLJ71903.1"
                     /translation="MSDSQQKKAFPVSWDQFHRDARALAWRLNSAGPFEAIVCVTRGG
                     LVPAAIVARELGTRVIETVCIASYHDYKTQGGLQVLKPISPDVVKLGDGKGVLIVDDL
                     VDTGATAKVVREMLPQAHFATVYAKPLGRPMVDTFVTEVSQDTWIYFPWDMGLAFQPP
                     IGKGAEG"
     gene            complement(8184..8972)
                     /locus_tag="VKU03_11350"
     CDS             complement(8184..8972)
                     /locus_tag="VKU03_11350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLJ71904.1"
                     /translation="MGAQEKIVTAAILVIGDEILSGRTKDRNIGYIAEYLTNMGVELR
                     EARVVPDVEDEIVAALNALRARYTYVFTTGGIGPTHDDITADAVAKAFGVRIGEDQRA
                     IALMLERYQPGELTEARRRMARIPEGAELIANPVSKAPGFRLGNVIVLAGVPTVMQAM
                     LDDAATHLQSGRKILAETIEAGNIAEGRYGGPLARIAEAHKSVTIGSYPAFSEGRFRN
                     QIVIRARDAALIAAARQDVEAMLAALGEERPPSSPAKAGEGDRA"
     gene            complement(8975..9850)
                     /locus_tag="VKU03_11355"
     CDS             complement(8975..9850)
                     /locus_tag="VKU03_11355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLJ71905.1"
                     /translation="MPFAAATSLSKAEAGDPSPFGRYAPSGFCAAALRVTRSCSNRWL
                     AKRLAFFLRSMSIKWLSGRPVDVETLGARMRLRPYNNVAEKRLLFTPQYFDAKERALI
                     ASRIGEDGVFIDAGASVGGYALSLAAIAPRAKILAIEPLPEIFERLVYNIDQNAFANV
                     KALSCALADRDGEVTLFVNANNRGETSVRIVSADAKGARLRVPAKSLLSVLRSEGYGR
                     LDAIKLDIEGAEDLALDAFFRDAPPRLWPRLIVMEFNLLTAEAELERRLRNLGYREIL
                     RTAENVAYEREAEAG"
     gene            complement(9995..10699)
                     /locus_tag="VKU03_11360"
     CDS             complement(9995..10699)
                     /locus_tag="VKU03_11360"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLJ71906.1"
                     /translation="MEIGFLVFPKVQQLDLTGPYEVFASWPEARVRMVARSLEPIVSA
                     TGLKLAPDTTFGACPQLDVLCVPGGVGINPLLADEETLSFIRRQAAGARFVTSVCTGV
                     LVLGAAGLIKGKRATTHWASHDLLAAFGAIPVQARVARDGALFTGGGVTAGIDFALTL
                     LAELAGRETAEAIQLALEYAPAPPFEAGTPESAPPAVLARQRARGASLRAERELLVAE
                     AARKLAGRGAPRAVSS"
     gene            10756..11745
                     /locus_tag="VKU03_11365"
     CDS             10756..11745
                     /locus_tag="VKU03_11365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HLJ71907.1"
                     /translation="MTETPRFPPEARAIEMVAFPNAQLLDIAGPLQAFATANDLARLA
                     RRPSPYSLKVVARQAGLLVSSAGLTLSAEPLPAPAEPLDTLIVAGGYGVDAACRDAGL
                     VAWLKARAAGARRLCSVCSGALLLAEAGFLAGRRAATHWGRCGELARKHPDVKVEPDP
                     IFVRDGHIWTSAGVTAGIDLALALIEEDLGRDTALAVARQLVVFLKRPGGQAQFSAAL
                     ALQAADERFEALHRWAAENLRADLSVPRLAEQAGMSERNFARRYKQATGLTPARAIER
                     LRVEAAQRRLCETGEPIKRIAARCGFGGEETMRRSFWRTAKIAPQDFRARFSR"
BASE COUNT         2037 a         3924 c         3963 g         1940 t
ORIGIN      
        1 tggcgccgga aaaatcaccc gatgctttac ctaatgagac ttcaagccgc cgaggcggac
       61 gccgtaccgt tgacgccgct tatgtcgcgg acttcgcaaa attgaaatct gcgcgatttt
      121 tcgcttcgct cgcaatgacg gccgcgccca acttagtccc aggttagctt gttgtcgtag
      181 atgtagagcg tcgagacttt gcaataatcg acatcggcgt cgaaatagga gccgtcggtg
      241 aaatggattt gcgtacgcac cgtgcagtcg cctttttccg tgccgaaatc catgtcgaat
      301 ttgtcgcccg gctcaatgcg atccttgatc caattggcgc tccactcgcc atcctcctgg
      361 gtgcggaact cgacggcggc gagattcgat ttgttgacga cgctgaactt ccaatggtcg
      421 ccggccgaag cggcggaagt cagagccgcg atgacaagcg ccgaagcgaa aagccaaaag
      481 cccctcatat ccccctcccc gtccgggccg ccaacgcgtc gcacggaccg cgccagaatt
      541 agatcaaggc gagcgaaccg ccaaggcggt tctccatttt caatatcaat cgctagccgc
      601 cggccggccg gatcatcgaa aacaaccgct cgacgacggc ataatcgtga tagccgcagc
      661 gggcgatcgg ctgcgccgcg gccgtgtcga accggccgtc cttgacgaac cggtcgtcga
      721 tatggacgcc gaccacctcg ccgagcacaa gatagcggtc gagggactcg gccttggcgt
      781 tcctgatctc gaccatctca gtgactttgc attcgagcgc ggcgcgcgcc tcggcgacgc
      841 gcggcgcggc gacgaggcgg cacggcgcca tggtcaggcc ggcatggagg aattcattgt
      901 cgccgcgggc cagcggcgcg gaggtggcgt tcatcggctg ggcgaggtcc attgtcgcca
      961 gattggcgac gaattcgccg ctttcgacgg cgaaggcggc cgaatcctta gcgccttcgc
     1021 tggaaaagcc gacgatcggc ggcgcgctgc aaaagccgtt gaagaagctg tacggcgcaa
     1081 gattgacgcg cccgtccttc gcccgggtcg agatccagcc gatcggccgc ggcgcgacaa
     1141 tggctttgaa cgggtcgtgc ggcagcagcg ccttgtcgcg ctgtctggtt tcgtagaaca
     1201 agcggctctc cccggttcgg cgcggcggtg gcgtggcttc gttcctcgct gcgctcgagt
     1261 cctcgcaaat gacgggcaaa taatcagaat cgcgtcacga tctcatccag cgtcgggcgg
     1321 gcgcggtcct cgatgacggc gctggggcgg ccgatatgga tgaagccggc gatcttctcg
     1381 tggccggcga ggccgagcgc attgcagaag cggcgatcat aggcgcacca ttcggtcacc
     1441 cagatgctct tatagcctaa cgcattggcg gcgatctgca ggttcatgca aaccgcgccg
     1501 gcggaaagca cctgctccca ttccgggatt ttgacatgcg gggcggcgcg ggagacgacg
     1561 gcgacgacga gcggggcgcg cgcgaagcgg ccgcgctccg cggcgatctt ggccgcgtcg
     1621 gcctgcggat tgtctcgcgc gaggagttcc gccgcgatcg ccccggcgcg gtcgcgcgcc
     1681 gcgccttcga aaagcaggaa acgccagggc gccagcttcc cgtgatcggg cacgcgcgag
     1741 gcgattctaa gaagggtttc aatctcctcc gcattcggac cggggccgtg aagggtctgc
     1801 ggcggcgccg agcgccggct gagcagcaag tcaatcgtcg ggttggtcat ggatctccaa
     1861 tcgcggggcg ccctctcggg cgtctttgcc ggcgttcatt ggccggtttg cgccctcctg
     1921 taaaccgtca cgaaaaggcc gatttgtcgt ggcgcctaac ggcgggcggc ggcattgacg
     1981 tgaggccgcg gcgcggccat tcaggggaac gatgcttcgt cttgttcagc ggctggcgct
     2041 ttggctgttg ctcggcgccg tggccgcggg tccggcggcc ggccagacga caattccgac
     2101 aaccctcccc ggcaaggcgg ttctgcacct ttcggccatt ctgagcgggc aggccgatcc
     2161 actgcgcggc ggcttgcatt ggcgcctcta cccggcgcgc gccgaggccg acggcgcccg
     2221 cgccctgatc gccgaatctg aggtcgccca gccgaccttc gtcgtgccgc cgggcgatta
     2281 cgtggtccat ctcgcgttcg gcctcgccgg ccagaccaag cgcgtcacgc tcggcgagga
     2341 ggagcgctcg gagcgcctga cgctcaacgc cggcgcgctc aaaatcgtcg gcacggtcgg
     2401 cggcgcggtc attccgtcga atcggctggc gctcgaaatt ttcgtgccgc agggccacaa
     2461 tcccgagggc aagctggtcg acgccaaagc ctcggccggc gaactgatcg gcctacccga
     2521 gggcccgtat cacatcgtct cgacctatct cgaccagggc gcgggaccat ccggcggcaa
     2581 gagcgccaat ccgccgatcc cgaccaattc gatcgtcgcc gccgatatca aagtccccgc
     2641 cgggaaggtg atcgatctca cattgcgcca ccgtttcgcg acgctgacgc tgaagctggt
     2701 caacgcgccc ggcgccgaag cgctcgccaa ttccagcttc accgtgctca cgccgggcgg
     2761 cgacctgatc cgcgaactgg tcggcgcttt tccgtcgctg gtcctggccg aaggcgaata
     2821 tatcgtcatc gcccggcacg accagaagac ctatcaaacc acgttccagg tgcaatcggc
     2881 gctcgaccgg gacgtggaag tcattgcaca atagagcgac tgaggggcgc ttttcgcgat
     2941 gcagacattt ttcgttcaga tcaaatgtgc gctggggcgc acttatgatg tcgccaacgc
     3001 cttggccgag gcggagatcg cctcggaaat ttattcgaca gccggcgatt tcgacattct
     3061 cgccaaattc tatgtggagc gcgagaccga tatcggccat ttcgtcggcc agaaggtgca
     3121 gatcattccc ggcatcgtcg acacgcggac gatcatcacc ttcaaggcct tctgaccggg
     3181 cggcgcttaa gcgcgccgct cgactccccg cgaagaaaga ttgaggaaag ggatcgatga
     3241 gacttgccgc cattctcgcc ttcacgctcg tcgccgggca ggccgcggcg cagaacggac
     3301 cttatctgtt cgacgtgctg ctcaaagggc cttaccggat cggatgggag aagctgctaa
     3361 aacaggttca gccgacgccg gactggctgt tgcgcttctc caaggatttc gacggcgtct
     3421 ccgggcaatt ggtcggcacg gcgatcgacg gcaaagccta tgagctctct ttcgtctgcg
     3481 agccgcaaca ttgcaaggcg cggcgcttcg aagtcatgtt tgaagtgtca acgaagcgcg
     3541 cctatggcgc cctctacttc gaaggcgagc cgccggcctt ttacggcgat ccgacgccgg
     3601 cgatgaagga ggagatgatc aaggcgctga agggctgatt gttgacgcgg ccgccgaccg
     3661 cgtcagccgc cctcgccgag cgcggctcca tcagtccgaa cagcagtccg tgtagaagtg
     3721 cgcgccgccg gcgggatcgc tgatctcgta cctcccctcc ccggccgccc gcgcatatcc
     3781 cggcccgatc ggcgccttgc catgcgcgcc gggtatgtcg agcacgtaag tcggctgggc
     3841 gagtccggag aggcggccgc gcaattggcg catcagcgcc tggccatcgg cgatcgtggc
     3901 gcgcagatgc gccgtgccgg gcgcgaggtc gccatgatgc agataatacg gcttgatcct
     3961 cgtctcgacc agcgcgcgca tcagcgcctc gagagtctcg acattgtcgt tgacgcccct
     4021 gagcagcacc gtctgactga ccatggccgc gccggcgccg atcaatctct tgcaggcggc
     4081 gcgcgccgca tcggtcagtt cgcgcggatg gttgacatgg agcgcgacat agaccgcctt
     4141 gcccggcgcg tccgccagcg cttcggccat cgcggaatcg accttctgcg gatcggcgac
     4201 cggcaggcgc gtgtgccagc gcagcacttt cacatggccg atttccgcca gcgcgcgcgt
     4261 cgccgcggcg aggcggcgcg gcgataaaac cagcggatcg ccgccggtca ccaccacttc
     4321 ccagatttcc tcatgctcgc gaagataggc gagggccgct tcgaactctt cggcgctgag
     4381 cgccggcgcg gcgcgcggcc cgaccgtttc gcggcgaaaa cagaagcggc aatagacggc
     4441 gcagaccgaa acgagcttca gcaaagcccg gtccggatag cggtggacta tacccttgac
     4501 cgggcttttc agctcatcgc cgatcggatc ggcgagctcg gccggatgcg cgatcagctc
     4561 gcggcggtcc ggaacgaatt gccgggcgat cggatcggcg ggatcagccg gatcgatcag
     4621 cgcggcgagc gccggcggga tcgcgacggc gtagcgctcg gcgacgcgcg cgatctcggc
     4681 cgcgcgcgcc tcggcgacga ggccggccgc gacaaggtcg gcggcggtct tgagggcgcg
     4741 cggcgcggcg gcgcgatcgg acgagaggct ttcgtgcatg ctgaggcttt ggcgtgtcgc
     4801 tcgggcgtct gatagccgga cgagcggtga aaacgcaaat gcggccgcct gtcattccgg
     4861 ctttcgcgca gggttgcccg gcgaaagtct ttgtgaggtc ttcgccaacg gcggtttcgc
     4921 gagacaggcc tgcgcttcag cggaatgaca cacgcgcgta cttcacccgc cgccagagcg
     4981 ctgatgcacc ggacccgcca ggctggcgag gttatgggcg gtgttgatca gcgcgagatg
     5041 cgagaaggct tgcgggaaat tgccgagctg ccgcttatcg gacggatcgt attcctcggc
     5101 gagcaatccg acatcgttgc ggagagcgag caaccgctcg aacagagcgc gcgcgtcgcg
     5161 atcgcggcca agcagaatgt agttgtcggc gagccagaag ctgcaagcca gaaacacgcc
     5221 ctcgcccggc ggcaggccgt cggtcttctt tccggtgtcg tagcggatca cgaaaccgtc
     5281 gcgcagcaaa ttgcgctcga tcgcggcgac cgtgcccttc acccgatcgt cgtcggccgg
     5341 cagaaagccg accatcggaa tcagcagcag gctggcgtcg agcgccgtcg cgccatagga
     5401 ctggacgaag gcgttctgcc gcggatcgta cccattgtcg cagacgtcct tgtgaatcgc
     5461 ggcgcggatc gcgcgccagc ggtcaagcgg cccatcgaga ccgaactctt cgatcgagcg
     5521 cacgacgcgg tcgaaagcga cccacgccat caccttcgaa tgggtgaagt gcctcgggcc
     5581 gccgcgcact tcccaaatcc cattgtccgg ctggcgccag attttttcta gatgctcgac
     5641 cagcgcgcat tccagcatcc agctcgccgc gtccggcgca aggccggcct tgcggccgac
     5701 ataaagcgcg tcgacgatct cgccatagac atcgagctgg agctgctcga cggcggcgtt
     5761 gccgacccgg accggctttg ccccgccata gcccggcagc caggggattt catattcgtt
     5821 gacgcgccgc tcgccggcga cgccatacat gatctggaga ttgtcgggcg atccgccgac
     5881 cgcccgcacc agccattcgc gccacgcctt ggcctcgtcg aggaagccgg cgccgatcag
     5941 cgcatagagc gtgaaggtcg catcgcgcag ccagcaatag cggtagtccc aattacgcga
     6001 gccgttcagc ttttccggca acgatgtcgt ggcggcggcg acgatgccgc cggtctccca
     6061 atgggccaag gccttgaggg tgagcagcga gcgcagaacc gcgtcgccgt attgctccgg
     6121 cggcttgaac gtcgccgccc attgcgacca gcgggacacg gcgccgtcga gcgccgtcct
     6181 ggcgtcgagc tcggccggcg gcgggcgaaa cgacggcgac cagttcaaac agaagctgat
     6241 ttcgtcgtcc gcgccgattt cgaattcgct cagcgtgcgg aaatcctcgc cgcgcgttgc
     6301 gactttcgtc gcgagaagga gccggtccgg cccggcgatg aattgcagac gcccgtccgg
     6361 ctggcgcgac acccagggca cgatcgcccc atattcgaag cggacgatca gctccatcct
     6421 catcgcgacc ttgccgctga ggccttgcac gaggcgcaat agctcggagg agccttcgcg
     6481 ccggatcatg aaatcgcaaa ggcgcacggc gccgtccggc gtctcgaact cggtctccag
     6541 gaccagcgtg tcgccgcgat agcgccgggc gaccttgcgc gattcatcgg cgggcgcgat
     6601 cagccagcgg ccatgcttgg gctcgccgag caaggcggcg aaacaggcgg cgctgtcgaa
     6661 gcgcggcagg ccgagccagt cgatcgaacc gtcgcggccg accaaagccg ccgtctcgca
     6721 atttccgatg acggcgtagt cttcgatccg gttcggcatg aggggccctg gagcgggagg
     6781 cgacggcgaa gcttgacgag gaaaagcgcc ccaataacgg aaatcgcggc cgccatgttc
     6841 cacatgccgc tgaggggcga aatcatggtg cggacggcgg gagtcgaacc cgcacagccg
     6901 aaaggccgag ggattttaag tcccttgcgt ctaccggttt cgccacgtcc gcgttgcgcg
     6961 gcccttctgt cacaaatcga ggggcatttg aactggatta gacatgggct attttcctcc
     7021 agagagcgaa aaatcgatga accaagtctt gagagagctc atccgacgcg ccgaggcgtg
     7081 gccagaatcc gctcgaggct gagctggcgg agatcgcccg agagatcgaa agcgagctcg
     7141 cgcgctccca cttcgcgacc ggcgaggaat tacgcgccct tcggcgagcg cggagaaacg
     7201 gcgagcgagt ctgccgtcga ggcggtgctg gcgaaatacc gccgcgcatg aaagttcggc
     7261 tctcagcggt cgcgcttgcc gagcttgacg aaattctcgc cgaaacatcc aggcatagcc
     7321 agcaaagtaa cgcaacgtcc cggcgggttc cgcgcgccac tggtccgcta tccttactcg
     7381 ctctacttca aaatcattcg cgaggagatt gtcgttctgc ggatccggcg tgatcggcgc
     7441 catccattat agcgcggccg ggcgcgcccc tatccttccg cccccttgcc gatcggcggc
     7501 tggaaggcga ggcccatgtc ccaggggaaa tagatccagg tgtcctgcga cacctcggtg
     7561 acgaacgtgt cgaccatcgg ccggccgagc ggtttggcgt agaccgtggc gaaatgcgcc
     7621 tgaggcagca tttcgcgcac cactttggcc gtcgcgccgg tatcgacgag atcgtcgacg
     7681 atcagcacgc ccttgccgtc gccgagcttg acgacgtcgg gcgagatcgg cttcagcacc
     7741 tggaggccgc cttgcgtctt gtagtcgtga taggaggcga tgcagaccgt ctcgatgacc
     7801 cgcgtgccga gctcgcgggc gacgatcgcc gccggcacca acccgccgcg ggtgacgcag
     7861 acgatcgcct cgaacggccc ggcgctgttc aggcgccagg cgagcgcgcg cgcgtcgcga
     7921 tggaactggt cccaggaaac cgggaaggcc tttttttgct gggaatctga catgggagcc
     7981 cgccgatcga atgacgcggg gcttgaatag caagcaaagg cccgcgtcgc gagaggcgaa
     8041 cacggatcga gcggatttcg cacaagattt gccgccgcgt tcctcccccg tcgaagacgg
     8101 ggaaggggga ccatgcgaag catggtggag ggggccgcgg cgcatcagga taatcccaac
     8161 cgccgaagcc ccctccaccg cgcttacgcg cggtccccct cccccgcttt cgcgggggag
     8221 gatggcggcc tctcctcgcc gagggcggcc agcatcgcct ccacatcctg gcgcgccgcc
     8281 gcgatcaggg ccgcgtcccg ggcgcggatg acgatctggt tgcggaagcg gccctcggaa
     8341 aaggccggat aggagccgat cgtcacgctc ttatgcgcct ccgcgatcct ggcgagcggg
     8401 ccgccatagc ggccttcggc gatgttgccg gcctcgatcg tctcggcgag gattttccgg
     8461 cctgactgca gatgcgtggc cgcatcgtcg agcatcgcct gcatcacggt cggcacgccg
     8521 gccaggacga tgacattgcc gagccggaag ccgggcgcct tggagaccgg gttggcgatg
     8581 agctcggcgc cttccgggat gcgggccatg cgccgccgcg cctcggtcaa ctcccccggc
     8641 tggtagcgct ccagcatcag cgcgatggcg cgctggtcct cgccgatcct gacgccgaac
     8701 gccttggcga cggcgtcggc ggtgatgtcg tcatgggtcg gtccgatgcc gccggtggtg
     8761 aagacataag tgtagcgcgc gcgaagagcg ttgagcgcgg cgacgatctc gtcctcgaca
     8821 tcgggtacga cgcgcgcctc gcgcaattcg acgcccatat tggtcaggta ttcggcgata
     8881 tagccgatat tgcggtcctt ggttcggccg gaaaggattt cgtcgccgat caccaggatc
     8941 gcggccgtga cgatcttctc ttgcgccccc attttcagcc cgcctccgcc tcgcgctcat
     9001 aggcgacgtt ttccgccgtg cgcaggattt cgcgatagcc gaggttgcgc aagcgccgct
     9061 ccagctccgc ctcggccgtc aggagattga actccatcac gatcaagcgc ggccataggc
     9121 gcggcggcgc gtcgcggaag aacgcgtcga gcgccagatc ctcggcgcct tcgatatcga
     9181 gcttgatcgc gtcgagccgg ccatagcctt cgctgcgcag cacagagagg aggctcttgg
     9241 ccggcacgcg caagcgcgcg cccttggcgt cggcgctgac gatccgtacc gaggtctcgc
     9301 cgcgattgtt cgcgttgacg aacagcgtca cctcgccgtc gcggtcggcg agcgcgcagg
     9361 agagagcctt cacattggcg aaggcgttct ggtcgatatt atagacaagc cgctcgaaaa
     9421 tctccggcag cggctcgatg gcgaggattt tcgcgcgcgg cgcgatcgcc gcgagcgaca
     9481 gagcgtagcc gccgacgctg gcgccggcgt cgatgaagac gccgtcctcg ccgatccgcg
     9541 aggcgatcag cgcgcgctcc ttggcgtcga aatattgcgg cgtgaacagg agccgctttt
     9601 ccgcgacatt gttgtagggg cgaaggcgca tgcgcgcgcc gagcgtctcg acgtcgaccg
     9661 gccgcccgct cagccatttg atgctcatcg agcgcaggaa aaaggccagc ctcttggcga
     9721 gccagcggtt cgagcaagag cgggtgacgc gcaaggccgc cgcgcaaaac ccgctcggcg
     9781 catagcggcc gaagggcgag ggatcgcccg cttcggcttt ggacaaggaa gtggcggcgg
     9841 cgaagggcat ggcgcttttc aaagcgcata gcatgcgcgg cgcggcgagc gccaggagcg
     9901 ccgggccgga aaaagcggct cagcgatcct gccgaaagga aggccgatcc gtgattttca
     9961 acaggtcgat ggagacccca tccaagcgta ctcttcagga agatacggcg cgcggcgcgc
    10021 cgcggccggc gagctttcgc gccgcttccg cgaccagaag ctcgcgctcg gcgcgcaagg
    10081 acgcgccgcg ggcgcgctgg cgcgccagga ccgccggcgg cgcgctttcc ggcgttccgg
    10141 cctcgaaggg cggcgccggc gcatattcga gcgcgagctg gatcgcctcg gccgtctcgc
    10201 gcccggcgag ctcggcgagc agcgtcagcg cgaaatcgat ccccgccgtg acgccgccgc
    10261 cggtgaagag cgcgccgtcg cgcgccaccc gggcctgaac cggaattgcg ccgaaggcgg
    10321 ccaggagatc gtgcgacgcc caatgggtcg tggcgcgctt gcccttgatg aggccggccg
    10381 ccccgaggac cagcacgcct gtgcagaccg aggtgacgaa acgcgcgccg gcggcctggc
    10441 ggcgaatgaa actcagcgtc tcctcgtcgg cgaggagcgg attgatcccg accccgccag
    10501 gaacgcaaag cacgtcgagt tgcgggcagg cgccaaacgt cgtgtcgggc gccagcttga
    10561 ggccggtcgc cgagacgatc ggttcgaggc tcctggcgac catccgcacc cgcgcttccg
    10621 gccaggaggc gaagacttcg taggggccgg tcagatcgag ctgctggacc ttggggaaga
    10681 cgagaaagcc gatttccatg gcgaagctcc ttcggagtgg cggcgagaag actaatcggc
    10741 tagaccttgg cggaaatgac agagacccct cgctttccgc cagaagcccg cgcgatcgag
    10801 atggtcgcct tcccgaacgc gcaattgctc gacattgccg ggccgctcca ggccttcgcg
    10861 acggccaacg acctcgcccg cctggcgcgg cggccctcgc cttattcgct gaaagtcgtc
    10921 gcccggcagg ccggcttgct cgtcagctcg gccggcctga cgctgagcgc cgagcccctg
    10981 cccgctccgg cggagccgct cgacacgctg atcgtcgccg gcggctacgg cgtcgacgcc
    11041 gcctgccgcg acgccggcct ggtcgcctgg ttgaaggcgc gggcggccgg cgcccgccgg
    11101 ctctgctcgg tttgctcggg cgcgctgctc ctcgccgagg ccggctttct cgccggacgg
    11161 cgcgccgcca cccattgggg ccgctgcggc gagctcgccc gcaagcatcc ggacgtgaag
    11221 gtcgagccgg acccgatctt cgtccgcgac ggccatatct ggacctcggc cggcgtgacc
    11281 gcggggatcg acctcgcttt ggctctgatc gaggaagacc tcggccgcga cacggcgctc
    11341 gccgtggcgc gccaactcgt cgtctttctc aagcggccag gcggtcaggc gcaattttcc
    11401 gccgccctcg ccttgcaggc ggccgacgag cgcttcgagg cgctccatcg ctgggccgcc
    11461 gagaatctgc gcgccgatct ctccgtgccg cgcctcgccg agcaggccgg gatgagcgag
    11521 cgcaatttcg cccggcgcta caagcaggcg accggcctga cgcccgcccg cgcaatcgag
    11581 cgcctcagag tcgaggcggc gcagcgccgg ctgtgcgaga ccggggagcc gatcaagcgc
    11641 atcgccgcgc gctgcggctt cggcggcgag gagacgatgc gccgcagctt ttggcgcacc
    11701 gccaaaatcg ccccgcagga tttccgcgcc cggttctcgc ggtgagcggc ccgctggcca
    11761 gccgagccgg atgcgcctat ataggcgcac agagagagga cgatccgtgg cgcaagcgga
    11821 gaccagccgg accaaaatca tccggcgtct tgaacaggaa ggtt
//
LOCUS       DATGJW010000374         4589 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_901030, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000374 DATGJW010000000
VERSION     DATGJW010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4589)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4589)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4589
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_901030"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(182..943)
                     /locus_tag="VKU03_11370"
     CDS             complement(182..943)
                     /locus_tag="VKU03_11370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transporter substrate-binding domain-containing
                     protein"
                     /protein_id="HLJ71908.1"
                     /translation="MTIPSFWDPDAQLERPELVGVRALRFLTDDDYPPLDFALPDGAL
                     AGFNVDIARAVCAELQVACTIQARRWDTLVDSLLAGKGDAIAASLAATPALRAKVDFT
                     KPYYKSPARFIVRKDSKLADATPATLAGKSVGVVEGSAHKAYLDAFFPGIVEKTFPDV
                     AALRAALRSGAIDAAFGDGLSSAVWLAGSASADCCLFKGGPYLESRFFGEGVGMALRK
                     EDADLRHAIDWAVARLAARGVYAELYLKYFPVGFY"
     gene            1189..3189
                     /locus_tag="VKU03_11375"
     CDS             1189..3189
                     /locus_tag="VKU03_11375"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ71909.1"
                     /translation="MGELVAKEETSERAPPPQPMGDTRPPRRAFDWTASLPPEIAFLA
                     HHDVAPGALLFAAFEAGKSGVSADQVLLNEGLMNEDDFYRLLAGHVRAPFYAGEIAID
                     AAIEPVEAVWSGAAALAPNDARLSHVVAPRGAAVKLLIRAAAAGVDPEGLAIASPRQF
                     LTSVTEAAGAKIAEAGALALKAQDAALSADRRVTAGQAAAAILFLATGAVLALGDADL
                     LKAIASIALWAVFGSSIALRLWASAQRPAPRERPCADRDLPVYSIVVALYREANMVDQ
                     LVAALDALDYPRAKLDVTIVLEQGDEQTLHALRRRGLAARYSVIVAPRGEPRTKPRAL
                     NIALPFMRGRYVVVYDAEDKPDRDQLRRAVARFEAEPDIGCLQARLAIEETGGQWLAT
                     MFAVEYAALFDALNPGLAELRAPIALGGTSNHFRTDVLRRVGGWDAWNVAEDADLGLR
                     LARFGVRVATFDSDTYEEAPAKLGHWLAQRRRWHKGWLQTALVHSRDPGRLLRELGPF
                     RAWGAASLMGGAVFGAMLGPFFTVYTLWRCAFGDLLAPRTWLQSMASAVSVAVLIAGL
                     FSVLAPAIVGLRRRGLDRLIWAAPLLPAYYVLISLAAWLALVDLCYRPFHWSKTEHGA
                     RRTPPPSRARPGSRRPFAIDIARRLPTVPPPFGRRAAHSRNA"
     gene            3191..>4589
                     /locus_tag="VKU03_11380"
     CDS             3191..>4589
                     /locus_tag="VKU03_11380"
                     /EC_number="6.1.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000049 - tRNA binding [Evidence IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysine--tRNA ligase"
                     /protein_id="HLJ71910.1"
                     /translation="MSPAALIDAAQASKAWPFEEARKLIRRIERTGQNEVLFETGYGP
                     SGLPHIGTFGEVARTTMVRHAFRVLTGDTIKTRLIAFSDDMDGLRKVPDNVPNQDMVR
                     RHLGKPLTQIPDPFGEYDSFAAHNNARLQKFLDAFGFDYEFASATAYYKSGRFDATLL
                     RVLERFEAVQQIMLPSLREERAATYSPFLPIHPETGIVMQVAIEAIDQKAGTIAWRDP
                     QSNRLFETKVTGGHCKLQWKPDWAMRWVALGVDYEMAGKDLIDSVRLSSQIARALGGT
                     PPEGFNYELFLDERGQKISKSKGNGLSIEEWLTYASPESLALFMYQKPSAAKRLYFDV
                     IPRTVDEYLSFLDAYPRQEMKERLGNPVWHVHSGAPPAPERLAHGAAGETSVSFGILL
                     NLAAVANSDNPAVLWGFLRRYAPSVSAETHPRLDKLVHYAVAYFRDFVAPKKRYRRAD
                     LIEEAALEALSAKLAE"
BASE COUNT          750 a         1542 c         1500 g          797 t
ORIGIN      
        1 tcgccgagaa gatcatctcg gcgatgcgca aaggcttcgg cggccatatc gagccgcatc
       61 acgagtagtt caggcgatca ttgcgagcga agcgaagcaa tccgtacgta gagcggcgcc
      121 ctatgaatgg attgcttcgg cgctgcgctc ctcgcaatga cggaggccgg tggattaagc
      181 ttcaatagaa ccccaccgga aagtatttca gataaagctc ggcataaacg ccgcgagcgg
      241 cgagccgcgc cacagcccag tcaatcgcat ggcggaggtc ggcgtcctcc ttgcgcagcg
      301 ccataccgac tccctcgccg aaaaaccggc tctcgagata ggggccgccc ttgaacaggc
      361 agcaatcggc ggaagccgag ccggcgagcc acaccgcaga ggagaggccg tcgccgaacg
      421 ccgcgtcgat cgcgcccgag cgcagcgcgg cgcgcagcgc cgcgacatcc ggaaaggtct
      481 tctcgacgat tccgggaaag aatgcgtcga gataggcctt gtgcgccgag ccttcgacga
      541 cgccgacgct cttgccggcc agagtcgccg gcgtcgcgtc ggccagcttc gaatctttcc
      601 gtacgatgaa acgcgccggc gatttgtaat agggcttggt gaaatccacc ttggcgcgca
      661 aagccggcgt cgcggcgagc gaagcggcaa tcgcatcgcc cttgccggcg agcagcgagt
      721 cgacgagcgt gtcccatcgc cgcgcctgga tcgtgcaggc gacttgcaat tcggcgcaga
      781 cggcgcgggc gatatcgaca ttgaaccccg ccagcgcccc gtcaggcagg gcaaaatcga
      841 gcggcggata gtcgtcgtcg gtcaggaagc gaagagcgcg cacgccgacc aattcgggac
      901 gttcgagctg cgcgtcagga tcccagaacg aggggatcgt cacccgcggc gccgcctgct
      961 gcgcagttgc ggcgatcgcg gagacaacaa ttgccgtaac ggcaagtaaa aatattttca
     1021 atttcatggt cggcgcagga tgacatcggc gcgaagctga gcttcactat agaagcggcg
     1081 gtttgggggc gtaatccttt ttttgacggg cgcttttggg atgcgcggcg gcgcagcccg
     1141 agctcggctt acgcgcggcc ttcgtcgcca caaaagagtg gtcgcggcgt gggggaactc
     1201 gtcgcaaaag aggaaacgtc cgagcgcgcg ccgccgcccc agccgatggg cgacacgcgg
     1261 ccgccgcgac gcgcgttcga ttggacggcg agcctgccgc cggaaattgc gtttctggcg
     1321 catcacgacg tcgcgcccgg cgcgttgctc ttcgccgcgt tcgaagccgg caagagcggc
     1381 gtctcggccg atcaggttct gctcaacgaa gggctgatga atgaggacga cttctatcgt
     1441 cttctcgccg gccatgtgag ggcgccgttc tatgccggcg agattgcgat cgacgcggcg
     1501 atcgagccgg tcgaagcggt atggagcggc gcggcggcgc tcgcgccgaa cgatgcgcgc
     1561 ctcagccatg tcgttgcgcc gcgcggcgcc gccgtgaaac tgctgatccg cgcggcggcg
     1621 gccggcgtcg atcccgaagg gctcgcgatc gcgtcgccgc gccaattcct gacttccgtc
     1681 accgaggcgg cgggcgcgaa aatcgccgag gccggcgcgc ttgctttgaa agcgcaggac
     1741 gccgcgctat cggcggatcg gcgcgtgact gccgggcaag ccgcggcggc gatccttttc
     1801 ctcgcaacag gcgccgttct cgccctcggc gacgccgatc tgctgaaagc gatcgcctcc
     1861 atcgcgcttt gggcggtttt cggctcgtcg atcgcgctgc ggctttgggc gtcagcgcag
     1921 cgcccggcgc cccgcgagcg gccctgcgcc gatcgcgatt tgccggtcta ttcgatcgtc
     1981 gtcgcgctct atcgcgaagc caatatggtc gatcagctcg tcgccgcgct ggatgcgctc
     2041 gactatccgc gcgccaaact ggatgtgacg atcgtcctcg aacagggcga cgagcagacc
     2101 ttgcacgcgc tgaggcggcg tggcttggcg gcgcgttata gcgtcatcgt cgcgccgcgc
     2161 ggcgagcccc ggaccaagcc gcgcgcgctc aacatcgctt tgcccttcat gcgcggccgc
     2221 tacgtcgtcg tctacgacgc ggaggacaag cccgaccgcg atcagctccg gcgggccgtg
     2281 gcgcgcttcg aggccgagcc ggatattggc tgcctacagg cgcgcctggc gatcgaggag
     2341 accggcgggc aatggctcgc cacgatgttc gcggtcgaat acgccgcctt gttcgatgcg
     2401 ctcaatcccg gccttgccga gttgcgcgcg ccgatcgcgc tgggcggcac gtccaaccat
     2461 ttccgcaccg acgtcctgcg gcgggtcggc ggctgggacg cctggaacgt cgccgaggac
     2521 gccgatctcg gcctgcgcct tgcgcgcttc ggcgtccgcg tcgcgacgtt cgattccgat
     2581 acctacgagg aggcgccggc gaagctcggc cactggctgg cgcagcgccg ccgctggcac
     2641 aagggctggc tccagaccgc tttggtccat tcgcgcgatc ccggaagatt gctgcgcgag
     2701 ctcggcccgt tccgcgcctg gggcgccgct tcgttgatgg gcggcgcggt tttcggcgcg
     2761 atgctcggcc cgtttttcac ggtttatacg ctgtggcgct gcgccttcgg cgatttgctc
     2821 gcgccgcgca cctggcttca atcgatggcg agcgccgtct cggtcgccgt gctgatcgcc
     2881 ggcctcttct ccgtcctggc gccggcgatc gtcgggctgc gccggcgcgg gctcgaccgc
     2941 ctcatctggg cggcgccgct tttgccggcc tattacgtcc tcatcagtct ggcggcgtgg
     3001 ctggcgcttg tcgacctttg ctaccggccg ttccactggt ccaagaccga gcatggggca
     3061 agacgaacgc cgccgccgag ccgggcgcgg cctggatcgc ggcgcccgtt cgcgattgac
     3121 atcgcgcgcc gccttcctac tgtgccgcct cctttcggcc gccgcgccgc tcattccagg
     3181 aacgcttgac atgtcgcccg ccgctctcat cgacgccgct caggcctcga aggcctggcc
     3241 gttcgaagag gcgcgcaagc tcatccgccg catcgagagg accggccaga acgaggtctt
     3301 gttcgagacc ggctatggcc cttcgggatt gccgcatatc ggcaccttcg gcgaagtcgc
     3361 ccgcacgacg atggtgcgcc acgccttccg cgtgctcact ggggacacaa tcaagactcg
     3421 gctgatcgcg ttttcggacg acatggacgg cctgcgcaag gttccggaca atgttccgaa
     3481 ccaggacatg gtccgccgcc atctcggcaa gccgctgacc cagatccccg atccgttcgg
     3541 cgagtacgac agtttcgccg ctcataacaa tgcgcggctg cagaaattcc tcgacgcgtt
     3601 cggcttcgat tacgaattcg cctcggcgac ggcctattac aagtcgggac gcttcgatgc
     3661 gacgctgctg cgcgtcctcg aacgattcga agcggtgcag cagatcatgc tgccgtcgct
     3721 gcgcgaggag cgcgccgcaa cctattcgcc atttctgccg atccatcccg agaccgggat
     3781 cgtcatgcag gtcgccatcg aggcgatcga ccagaaggcg ggaacgatcg cctggcgcga
     3841 cccgcaatcg aaccgtctct tcgagacgaa ggtgacgggc ggccattgca aattgcaatg
     3901 gaagccggat tgggcgatgc gctgggtcgc gctcggcgtc gactatgaaa tggccggcaa
     3961 ggatttgatc gactcggtgc gcctctcctc gcagatcgcc cgcgcccttg gcgggacgcc
     4021 gcccgaaggg ttcaactacg agcttttcct cgacgagcgc ggccagaaga tttcgaagtc
     4081 gaagggcaac ggcctgtcga tcgaggaatg gctgacctac gccagccccg aaagcctggc
     4141 tctgttcatg taccagaagc cgagcgcggc caagcgcctc tatttcgacg tgatcccgcg
     4201 cacggtcgac gaatatctct cctttctcga cgcctatccg cgccaggaga tgaaggagcg
     4261 gctcggcaat ccggtctggc acgtccattc cggcgcgccg ccggcgccgg agcggctggc
     4321 gcatggcgca gccggcgaga cctccgtttc cttcggcatt ctgctcaatc tcgccgcggt
     4381 cgccaacagc gacaatccgg cggtgctgtg gggcttcctc cgccgctatg cgccctcggt
     4441 ctcggccgaa acgcatccgc gcctcgacaa gctggtccat tacgccgtcg cttatttccg
     4501 cgatttcgtc gccccgaaaa agcgctatcg ccgcgccgac ctcatcgaag aggccgccct
     4561 tgaagcgctc tcggccaagc tcgccgaat
//
LOCUS       DATGJW010000375         4935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_383784, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000375 DATGJW010000000
VERSION     DATGJW010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4935
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_383784"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1243)
                     /locus_tag="VKU03_11385"
     CDS             complement(<1..1243)
                     /locus_tag="VKU03_11385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669795.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HLJ71911.1"
                     /translation="MTTHQRNFSASIDAAKLDRLAETAIKIGLRLQPGQDLFLTAPIA
                     ALPLARRIAEQAYKAGAGLVTPIFSDEEMTLARYRFAPEESFDRAAGWLYDGVAKAFA
                     ANTARLAIVGENPMLLAEQDPAKVARANKANSLAYQPALEKIAGFDINWNIVAYPTAS
                     WAKLVFPDEQDEIAVAKLAHAIFAASRVDGDDPIAAWSAHNSALRSRTEWLNGKKFQA
                     LHYSGPGTDLTIGLADGHEWQGGASTAKNGIVCNPNIPTEEVFTTPHARRVDGHVSST
                     KPLSYQGSLIDNIRVRFEAGRIIEAKASRGEEVLNKVLDTDDGARRLGEVALVPHSSP
                     ISKSGLLFFNTLFDENAACHIALGQCYSKCFLDGAKLSQEQIATRGGNKSLIHIDWMI
                     GSGELDIDGIGADGGRTPVFRK"
     gene            complement(1366..2091)
                     /locus_tag="VKU03_11390"
     CDS             complement(1366..2091)
                     /locus_tag="VKU03_11390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLJ71912.1"
                     /translation="MSPQTPKTAMVFAAGLGLRMRPITETLPKPLVKVAGRTLIDHCL
                     DRLAEAGVERAIVNVHYRADQIEAHLAARRRPEIIVSDERGKLLDQGGGIKKVLPLIG
                     DEPFLLCNTDAFWLEGPRSNLRRLAAAWDAERMDAALLVAPTVSAVGVDWPGDFEMSA
                     DGWLARRKERDVAPYVYAGIGIIKPDLFRREPREAFRLAPFFFEAAEKGRLLGVRLDG
                     VWLHVGYPDAIAEAERAIDRSTL"
     gene            complement(2252..3799)
                     /gene="tsaE"
                     /locus_tag="VKU03_11395"
     CDS             complement(2252..3799)
                     /gene="tsaE"
                     /locus_tag="VKU03_11395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009490434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HLJ71913.1"
                     /translation="MPPQTRSSPADPIWRIDLPNEQATEAFAARLAEWLKPGDLVALS
                     GDLGAGKTTLARALIRKLTGDPTLEAPSPTFTLMQTYDGPGYPIVHADFYRIKHPDEL
                     VNLGWDEAIESALVLVEWPQRAEAALKPDRFEIALALAPQSGPDARRAILIGHGAFAT
                     RVARARGIASVLAEAGWSGAQRVFMQGDASIRAYEKLIKPSGETAILMISPPRPDGPI
                     LRYGKPYAAIAKLAEDIRAFVAMAEGLRAQGFSTPRLFAHSIADGAAVIEDLGDQFIA
                     GPDGPIAERYSLAVDLLAELHSRALPSELPVDGETYALRPYDGEAMLVEAELALDWYA
                     PYVAKVAPASGARAQFLSVWRDALAPILAEPTTWTLRDYHSPNLLWLPERDGLARIGL
                     IDFQDCVLGPPAYDVAALLQDARVDVGEELELKLMAQYTRRRLAKDPAFDVAAFAAAY
                     AAMGAQRATKILGIFARLDRRDGKPQYLAHLPRIERILAKSLAHPLLTPIRAWHEAHL
                     PQALGAV"
     gene            complement(3803..>4935)
                     /locus_tag="VKU03_11400"
     CDS             complement(3803..>4935)
                     /locus_tag="VKU03_11400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ71914.1"
                     /translation="ESQFNALIRVQGETLDTLKEGVAVFGMDGRLKLSNPAFVELWRL
                     DPARIARRPHIDEMARLCSTLYADTGLWGALRDVIVGMPEVRAGYEKRIERDDGVIID
                     CAAMPLPDGATLITFLDVTAGVNVERTLTKLNKALMEAEQLRNAFIRRVSYELRSPLT
                     SIIGFTQLLADGAVGPLNEKQLEYAGHVTKSSAALLAIIDDILDLASIDAGALELKLE
                     DVDVAEAMKAAAEGVQDRLTESGIELRIIATDGVGIMRADGLRVRQILFNLLSNAIGF
                     SEAGQTVTLAAMRRGGEIVFKVSDRGRGIPPDVMDRIFTPFETFTAGSRHRGVGLGLS
                     IVRALVELHGGRLLIDSAPDEGTTVTVIFPTEGAAAAASRVA"
BASE COUNT          806 a         1561 c         1743 g          825 t
ORIGIN      
        1 ctttccggaa caccggcgtc cgcccgccat ccgcgccgat tccgtcaatg tcgagctcgc
       61 ccgagccgat catccaatcg atatggatca agctcttgtt gccgccgcgc gtcgcgattt
      121 gctcttggct cagcttggcg ccgtcaagga agcatttcga atagcattgg ccgagcgcga
      181 tgtggcaggc ggcgttctcg tcgaacagcg tgttgaagaa caagaggccg cttttcgaga
      241 tcggcgagga atgcggcacc agcgcgactt cgccgagccg gcgcgcgccg tcatcggtgt
      301 cgagcacctt gttgagtact tcctcgccgc gcgaggcttt cgcctcgatg atgcggccgg
      361 cttcgaaacg cacccgaata ttgtcgatca gcgagccttg gtacgaaagc ggcttggtgc
      421 tgctgacatg cccgtccacg cggcgcgcat gcggcgtggt gaacacctcc tcggtcggga
      481 tgttcggatt gcagacgatt ccgttcttcg cggtcgaggc gccgccctgc cattcgtggc
      541 cgtcggcgag gccgatggtg aggtccgtgc ccggcccgga ataatgcaag gcctggaatt
      601 ttttgccgtt cagccattcc gtcctgctgc gcaaggcgga attgtgcgcg ctccaggcgg
      661 cgatcggatc gtcgccgtcg acgcgcgagg cggcgaaaat cgcatgcgcg agcttggcca
      721 cggcgatctc gtcctgctcg tctgggaaga ccagcttcgc ccaggacgcg gtcggatagg
      781 cgacgatgtt ccaattgatg tcgaagccgg cgatcttctc caaagccggc tgataggcga
      841 gcgagttggc cttgttggcg cgcgccactt tcgccgggtc ctgctcggcg agcagcatcg
      901 ggttttcgcc gacaatggcg agccgggccg tgttggcggc gaaggccttg gcgacgccgt
      961 cgtaaagcca gccggcggcg cggtcgaaac tttcctccgg cgcgaagcgg tagcgcgcca
     1021 gcgtcatctc ttcgtccgag aagatcggcg tcaccagccc ggccccggcc ttgtaggctt
     1081 gctcggcgat ccgccgcgcc agcggcagcg cggcgatcgg cgcggtgagg aaaaggtcct
     1141 ggcctggttg cagcctgagc ccgatcttga tcgccgtctc ggcgaggcgg tcgagcttgg
     1201 cggcgtcgat cgatgcggag aagttgcgct gatgggtggt catggaggct ccgccggatg
     1261 gtggtcgttc aggggtgacg gcgagaatat agttctcgaa cggcgattgg gctcacaaat
     1321 tcggatcggg cttttcgccg ttgtggccgc ggtcgccgac cgcggctaca gggtagagcg
     1381 gtcgatcgcc cgctccgcct cagcgatggc gtcgggatag ccgacatgca gccagacgcc
     1441 gtcgagccgg acgccgagca ggcggccctt ctcggcggct tcgaagaaga agggcgcgag
     1501 ccgaaaggcc tcgcgcggct cgcgcctgaa cagatcgggc ttgatgatgc cgatcccggc
     1561 atagacatag ggcgcgacgt cgcgctcctt gcggcgcgcc agccagccgt cggcgctcat
     1621 ctcgaaatcg cctggccagt cgacgccaac cgcgctcacc gttggcgcga cgaggagcgc
     1681 cgcgtccatc cgctccgcgt cccaggcggc ggcgaggcgc cttaaattcg agcgtggccc
     1741 ttcgagccag aacgcgtcgg tgttgcagag caggaaaggc tcgtcgccga tgagcgggag
     1801 aaccttcttg atgccgccgc cttgatcgag cagcttgccg cgttcgtccg agacgatgat
     1861 ttcgggccgc cgccgcgcgg cgagatgcgc ttcgatctga tcggcccggt aatggacatt
     1921 gacgatcgcg cgctcgacgc cggcttcggc caagcggtcg aggcaatgat cgatcagcgt
     1981 cctcccggcg actttcacca aaggcttcgg cagcgtctcg gtgatcggac gcatgcgcaa
     2041 gccgaggccg gcggcgaaga ccatcgccgt cttgggcgtc tgcgggctca tgaagggcga
     2101 ctcatgtgaa gggctgactg cgagcgcggc ttcggcgcga gcggagattg cggccgcggt
     2161 cggcgagcgc gactacagcg cggcgttccg agcgaggccg tggcgcggct tgcggggaaa
     2221 gggcttggct gaaggcgggc ggcgttcatt gtcaaaccgc gccgagcgcc tgcggcagat
     2281 gagcttcgtg ccaggcgcgg atcggcgtca ggagcgggtg ggcgaggctc ttggccagga
     2341 tgcgctcgat acgcggcaga tgggcgagat attgcggctt gccgtcgcgc cggtcgagcc
     2401 tggcgaatat gccgaggatt ttcgtcgcgc gctgggcgcc catcgcggcg taggcggcgg
     2461 cgaaggcggc gacgtcgaaa gccgggtcct tggcgaggcg ccgccgagta tattgcgcca
     2521 tcagcttgag ctccaactcc tcgccgacat cgacgcgcgc gtcctgcagc agcgccgcga
     2581 cgtcgtaagc gggcggcccg agcacgcaat cctggaaatc gatcaggccg atccgggcga
     2641 ggccgtcgcg ctccggcagc caaagcagat tgggcgagtg atagtcgcgc aaggtccagg
     2701 tcgtcggctc ggcgaggatc ggcgccagcg cgtcccgcca gacgctgagg aattgcgcgc
     2761 gcgcgccgga agccggcgcg accttggcga cgtatggcgc gtaccagtcg agcgccagct
     2821 cggcctcgac cagcatcgcc tcgccgtcat agggacggag cgcataggtc tcgccgtcga
     2881 ccggcagttc ggacggcaag gcgcggctat gcaattcggc gagcaggtcg acggcgaggc
     2941 tgtagcgttc ggcgatcggg ccgtccgggc cggcgatgaa ttgatcgccc agatcctcga
     3001 tcaccgcggc gccgtcggcg atgctgtggg cgaaaaggcg cggcgtcgaa aagccctgcg
     3061 ctcttaagcc ctcggccatc gcaacaaagg cgcggatgtc ctcggcgagc ttggcgatcg
     3121 ccgcataagg cttgccgtag cgcaggatcg gcccgtcggg ccgcggcggc gagatcatca
     3181 agatcgccgt ctcgccggac ggcttgatca gcttctcata ggcgcggatc gaggcgtcgc
     3241 cctgcatgaa gacgcgctgg gccccgctcc agccggcttc ggcgagaacc gaggcgatcc
     3301 cgcgcgcccg cgccacccgg gtggcgaagg cgccgtggcc gatcaggatc gcgcggcgcg
     3361 cgtccggacc cgattgcggc gccagagcga gggcgatttc aaaccggtcc ggtttgagcg
     3421 ccgcttccgc gcgctgcggc cattcgacca aaaccagcgc gctctcgatc gcctcgtccc
     3481 agccgagatt gaccaattcg tcgggatgct tgatgcggta aaagtcggca tggacgatcg
     3541 gatagccggg gccgtcatag gtctgcatca aggtgaaggt cgggctcggc gcctccaggg
     3601 tcgggtcgcc ggtaagcttg cgaatcaaag cgcgcgccaa agtcgtcttg ccggcgccga
     3661 gatcgccgga aagggcgacg agatcgccgg gcttgagcca ttcggcgagc cgcgcggcga
     3721 acgcctcggt cgcctgctcg ttcggcagat cgatgcgcca gatcggatcg gccggactcg
     3781 aacgcgtctg cggcggcatg gcttaagcca cccggctcgc cgcggccgcc gcgccttcgg
     3841 tcgggaaaat gacggtgacg gtggtgccct cgtcgggcgc cgaatcgatc agcaggcggc
     3901 cgccatgcag ctcgaccagc gcgcgcacga tcgacaggcc gaggccgacg ccgcgatggc
     3961 gcgagccggc ggtgaatgtc tcgaacggcg tgaaaatgcg gtccatgacg tccgggggaa
     4021 tgccgcggcc gcgatcggag accttgaaga cgatttcccc gccgcgccgc atcgcggcga
     4081 gggtaacggt ttggcccgcc tccgaaaagc cgatggcgtt ggagagcagg ttgaacaaaa
     4141 tctgccgcac ccgcaagccg tcggcgcgca tgatcccgac gccgtcggtt gcgatgatgc
     4201 gaagctcgat ccccgattcg gtgaggcggt cctgcacgcc ttcggcggcg gccttcatcg
     4261 cctcggcgac gtcgacgtct tccagcttga gctcgagcgc gccggcgtcg atcgaggcga
     4321 ggtcgagaat gtcgtcgata atggcgagca aagcggctga ggatttcgtc acatggccgg
     4381 catattcgag ctgcttctcg ttgagcggac cgaccgcgcc gtcggcgagc aattgcgtga
     4441 agccgataat gctggtgagc ggcgagcgca gctcgtagct gacccggcgg atgaaggcgt
     4501 tgcgaagctg ctccgcctcc atcagggcct tgttgagctt ggtcagggtg cgctcgacat
     4561 tcacgccggc ggtgacgtcg aggaaagtga tcaatgtcgc gccgtcgggc agcggcatgg
     4621 cggcgcagtc gatgatgacg ccgtcatcgc gctcgatgcg cttctcgtag ccggcgcgca
     4681 cttccggcat gccgacgatc acgtcgcgca gcgcgcccca gaggccggta tcggcataga
     4741 gggtcgaaca gagccgcgcc atttcgtcga tatgcgggcg ccgggcgatc ctggccggat
     4801 cgagccgcca cagttcgaca aaagcagggt tggacagttt caggcggccg tccatgccga
     4861 aaaccgcgac gccctcttta agcgtgtcca gcgtctcgcc ctggacgcgg atcagcgcat
     4921 tgaactgcga ttcca
//
LOCUS       DATGJW010000376         3741 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1419860, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000376 DATGJW010000000
VERSION     DATGJW010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3741)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3741)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3741
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1419860"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..699
                     /locus_tag="VKU03_11405"
     CDS             <1..699
                     /locus_tag="VKU03_11405"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLJ71915.1"
                     /translation="YWPSAISIENYLKVLHYQAGLLVYVRNSLIVAALTIALCLGLGA
                     PAAFGLARFPVPAKEVVFLVLLSALMIPYQALLTPLYTMAASLGLANTYLGLAIVHTV
                     IQLPFSVFLLRNSFEEAPRELEEAAIIDGCNSLQLLRWIFLPAAAPAIVTVTLFAFIT
                     SWNEFLAALIFMNKETAFTVPIMLVGVRQGHFGAVDWGALQAGVVVSILPCLAIYLLL
                     QRYYVSGFLSGAIK"
     gene            714..1799
                     /locus_tag="VKU03_11410"
     CDS             714..1799
                     /locus_tag="VKU03_11410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007798284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLJ71916.1"
                     /translation="MASQDEAPPALSGRAAGGARDVRRQPTIHDVARLAKVSIGTVSK
                     ALNGNGRLSAETRARVNALARQVGFRPNHLAQSLHRGQSFTVGVISNDNFGRFAMPIM
                     EGLEECLAEQRISVFMCNATDDPALEARHVEQLLAKRVDGLVVTGRRADRRPPLQTPL
                     GGLPILYVFSQTDDPDALCLLPDDFGGALTATEHLVRLGRRRIAHVTGPERFEAVRLR
                     REGWRAALGSAGLPASDHRCLAGAWSEAWGREALDKLLAGKRPAPDAIFCGNDQIARG
                     VADALRERGLKAPQDVAIVGFDNWLVIAEATRPLLTSVDMNLKELGREAGRRLMAMIA
                     GEKQSGARRLPCSLIVRDSCGARLTAR"
     gene            1903..2079
                     /locus_tag="VKU03_11415"
     CDS             1903..2079
                     /locus_tag="VKU03_11415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71917.1"
                     /translation="MQPPEKIWKILGSREKRRWGEFRVQENPFLIKEAAKPPPNIAKF
                     FQNFSWPFRAIPKT"
     gene            complement(2368..2982)
                     /locus_tag="VKU03_11420"
     CDS             complement(2368..2982)
                     /locus_tag="VKU03_11420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71918.1"
                     /translation="MTKPFSLIVTDTSPLLTLALADALDALLRPGLAVTIPDAVYIEA
                     TRVHGAPGAEQIVEWINAHLDMVRIIPTDIGIDQQRRLEEGRPMRGLGEQAAIEALDR
                     FLRSDAEAEALLLFEDSDVQKRRAIVEARVSLISTGDFLRELEAARLIQSADFILDQA
                     AARGRNVERQRQAGSDAATRERLREQLQRRAWVGLALIASPQRA"
     gene            complement(2979..3200)
                     /locus_tag="VKU03_11425"
     CDS             complement(2979..3200)
                     /locus_tag="VKU03_11425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71919.1"
                     /translation="MAETDKQKLIRQYAAGEITWRELRERGFRDYVEVLGALGEPGPR
                     PPIAPMEGPNVEARRRGRALIREALRARK"
BASE COUNT          599 a         1262 c         1201 g          679 t
ORIGIN      
        1 tactggccgt cggcgatcag catcgagaat tacctcaagg tgctccacta tcaggccggc
       61 ctgctggttt acgtccgcaa cagccttatc gtcgccgcgt tgaccatcgc gctctgcctc
      121 ggcctcggcg cgccggccgc cttcggtctc gcccgcttcc cggtgccggc gaaggaggtc
      181 gtctttctcg tcctgctgtc ggcgctgatg atcccgtacc aggcgctgct gacgccgctc
      241 tacacgatgg cggcctcgct cggcctcgcc aacacctatc tcggcctcgc catcgtccac
      301 accgtcatcc agttgccgtt cagcgtcttt ctgctgcgca attcgttcga agaggcgccg
      361 cgcgaactgg aggaggcggc gatcatcgac ggctgcaact cgctgcaact cctgcgctgg
      421 attttcctgc ccgccgccgc gccggcgatc gtcacggtaa cgctgttcgc cttcatcacg
      481 tcctggaacg aattcctcgc cgccctgatc ttcatgaaca aggagacggc gttcaccgtg
      541 ccgatcatgc tggtcggcgt gcggcaaggc catttcggcg ctgtcgattg gggcgcgctg
      601 caagccggcg tcgtcgtctc gatcctgccg tgcctggcga tctatctcct gttgcaaaga
      661 tactacgttt ccggcttcct gtccggcgcg atcaaatagg aaggcgcggg gacatggcca
      721 gccaggacga agcgccgccg gcgctgagcg gccgcgccgc aggcggcgcg agggacgtgc
      781 ggcgccaacc gaccatccac gacgtcgccc gcctcgccaa agtcagcatc ggcaccgtgt
      841 cgaaggcgct caacggcaac ggccgcctga gcgccgagac gcgcgcccgc gtcaacgcgc
      901 tcgcccggca ggtcggcttc cggcccaacc atctcgccca gagcctgcat cgcggccaat
      961 ccttcaccgt cggggtgatc tcgaacgaca atttcggccg cttcgccatg ccgatcatgg
     1021 aggggctgga ggaatgcctg gccgagcagc gcatctcggt cttcatgtgc aacgcgaccg
     1081 acgatccggc gctcgaagcg cgccatgtcg agcagttgct cgccaagcgg gtcgacggcc
     1141 tcgtcgtgac cggccgccgc gccgaccgcc gtccgccctt gcagacgccg ctcggcggcc
     1201 tgccgattct ctatgtcttc tcgcagaccg acgatcccga cgccttgtgc ctcttgccgg
     1261 acgatttcgg cggcgcgctc acggcgacgg agcatctcgt ccggctgggc cggcgccgca
     1321 tcgcccatgt caccgggccg gagcgattcg aggcggtgcg gctcaggcgc gagggctggc
     1381 gcgccgcgct gggaagcgcc ggccttccgg cgtcggacca tcgctgcctc gccggcgcct
     1441 ggtcggaggc ctggggccgc gaggcgctcg acaagctcct cgccggcaag cgcccagcgc
     1501 cggacgcgat cttttgcggc aacgaccaga tcgcccgcgg cgtcgccgac gccttgcgcg
     1561 agcgcggcct gaaggcgccg caagacgtgg cgatcgtcgg cttcgacaat tggctggtaa
     1621 tcgccgaagc gacacggccg ctgctgacga gcgtcgacat gaacctgaag gagctcggcc
     1681 gcgaggccgg ccggcgcctg atggcgatga tcgccggcga gaaacagagc ggcgcgcgcc
     1741 gcctcccctg cagcctgatc gtgcgcgatt cctgcggggc gcggctcacg gcgcggtgat
     1801 tcgcgctttg ctcgtcattg cgggcgaagc gaagcaatcc aggaagcgcc ggcgtctcct
     1861 ggattgcttc gtcgctgcgc tcctcgcaat gacggcgtac tcttgcagcc gcccgagaag
     1921 atttggaaaa tccttggatc gcgcgaaaag cgccgctggg gcgaatttcg cgttcaggaa
     1981 aatccctttc ttatcaaaga ggccgcaaaa ccgccgccaa acatcgccaa atttttccaa
     2041 aacttttctt ggccgtttcg cgcgattcca aagacttaaa gggccgaaaa gcttgggaga
     2101 ggctttttca aggtccttgt cgctcgtacc tcttcggctt cttcgcagcc gcgtattata
     2161 cgcgagaaag aatcgtccag gaagaatttg ttcgtacctt tatcctaaat cactgatcga
     2221 gtttcactat ttcgatagat ccagtcaacg ccgacgccag ggtgggccgt cggcgagcgc
     2281 gctggttctg tagccgcggt cgacgaccgc ggcagcgccg gctcgccgag gccggctaca
     2341 gaccggcgcg ccagcgcgac gcggcggtca cgcccgttgc ggcgatgcga tcagcgctaa
     2401 tccgacccag gcgcggcgct ggagctgctc gcgcagcctc tcgcgcgtgg cggcgtcgct
     2461 gccggcttgg cgctgccttt cgacgttgcg accgcgcgct gcagcctggt cgagaataaa
     2521 atcggccgac tggatgagtc gcgccgcttc gagttcgcgc aaaaagtcgc ccgtgctgat
     2581 gagactcacc cgcgcctcga cgatcgcgcg gcgcttttgc acgtcgctat cctcaaacaa
     2641 caaaagcgct tcggcctccg cgtcgctgcg caagaaccga tcaagcgcct cgatggcggc
     2701 ttgttcgcca aggccgcgca tcgggcgtcc ttcttcgagg cgtcgctgtt gatcgatgcc
     2761 gatgtcggtc ggtataattc gcaccatgtc gagatgggcg ttgatccatt cgacgatctg
     2821 ctcggcgcca ggcgcgccat gcacgcgcgt ggcttcgata tagaccgcat cgggtatggt
     2881 gacggccaac cctggccgca gcaaagcgtc cagcgcgtcg gcgagcgcca atgtcagcag
     2941 cggcgatgtg tcggtgacga tgaggctgaa aggcttcgtc acttgcgcgc ccgcagggct
     3001 tcgcgaatca gcgcgcgccc gcgccggcgg gcctcgacat tggggccttc catcggcgcg
     3061 atcggcggac gcgggccggg ctcgccgaga gcgccgagca cttcaacgta atcgcgaaag
     3121 ccgcgctcgc gcaactcacg ccaagtgatt tcgccggcgg cgtattggcg gatgagcttc
     3181 tgcttgtcgg tctcggccat gggcgcgttc tgcctgttcg cgaaggaatt atagtcgagg
     3241 ctggaagaag aaactatcga aacgcgtatc aggccagcgc gaggtcacgg ttctgtagcc
     3301 gcggtcgatg accgcggcag cgccggcctc agcgaggccg gctacaggtc agcgccagcg
     3361 cgtcgtggcc gataaaccct aagcgccaat gccgccgtgc tcgccggcgt caagccgcgc
     3421 tcctcgcaaa tcctcgccat gctcgcttcg cttcgcgaag cttgcgcgtg gctccgggtt
     3481 gctccggggc gcagcttgac cccgcctgcg cgcggcggcc ttctggaggg gtcgggacga
     3541 ggggcgtccg cccctcgctc cgcgaggaag ttcgacttgc cctgcgggcg gcgtcgaact
     3601 aggggcggca tggacgccgc gctggcttcg gcggactgtt taagttttta acagtcccca
     3661 aaatcaccga gaccggacat cggccgcaat ttgtttggga cattcctcga ttagaaactg
     3721 atcgggcgat aggtcgtcgt c
//
LOCUS       DATGJW010000377         4123 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_384139, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000377 DATGJW010000000
VERSION     DATGJW010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4123)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4123)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4123
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_384139"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            27..794
                     /locus_tag="VKU03_11430"
     CDS             27..794
                     /locus_tag="VKU03_11430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ71920.1"
                     /translation="MAVRDLHVEVGEQKVLKGLTLDVYRGEILGFVGASGAGKSVLMR
                     TIIGLLPKRRGTISVLGVDRDRASEAERQAVERRWGILFQQGALFSNLTVKQNVQAPM
                     REHLGISKRLRDELAMLKIALVGLSPDAAEKYPSELSGGMIKRAALARALALDPDILF
                     LDEPTSGLDPIGAGEFDELLATLQKTLGLSVFMVTHDLDSLYAVCDRVAALGQGRVIA
                     AGPIADLLNSDDPWLKAYFHGKRGAGREAAQARRGVE"
     gene            942..2570
                     /locus_tag="VKU03_11435"
     CDS             942..2570
                     /locus_tag="VKU03_11435"
                     /inference="COORDINATES: protein motif:HMM:NF014523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MlaD family protein"
                     /protein_id="HLJ71921.1"
                     /translation="METRANYVLIGVFTLAVIAGGFLFVMWFSGLGRGAQNKTYEVVF
                     TGSVSGLSRGSLVSFNGLRVGEVTQISFFPNDPSRVAAMINVVANTPVKTDTKARLES
                     QGLTGVATLALTGESSSAPPLEPGPGGAPPVIKAERSDFQNLLETVQRISGKADEVLT
                     KANKLLDENGPAVGDALRNVDEFSKALADNSAGVKTFLASVGDLGQKLAPLSSKLETL
                     SSDVDAAVKAVDPAKVKNILANADDFSAALARNKDNIDTTLAGAAAIAKRLDGTSQKL
                     DATLDNLAAFSATLAGNKANIDSLLADSAALAKRLGGTSDKLDAALDQVTGLVKSVDR
                     DKVAGIVDNVSGFSQTLSRNQGNIDSLLADSAALAKRLNGTSQKLDNALDQVSDLAKA
                     FDRAKIASAVDNVAEFTDALKQNRGNVDHMLKDAAELAAKLNGSADQIDGLLRSLQGL
                     IGSPDSKGAIAQVGDAARAIRQLAENLDQRVKEISVGLTRFSGSGLREYEALAIDGRR
                     TVNDIDRFVHSLSNNPSQVIFGAKPALPDYHAGQ"
     gene            2591..3184
                     /locus_tag="VKU03_11440"
     CDS             2591..3184
                     /locus_tag="VKU03_11440"
                     /inference="COORDINATES: protein motif:HMM:NF015823.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-type transport auxiliary lipoprotein family
                     protein"
                     /protein_id="HLJ71922.1"
                     /translation="MFGPVGRARRLVAAALALPLVLGLAACGGGASLPSFDLSATSAP
                     SERPLRASLIVRQPLSPLAFDSRRIVVRTGPDALAYLADAQWSDRLPVLVQSRLAETF
                     QNAHLFRSVERASKPADYALELEIRHFEIDAPSRAGIVEIAAKIVSGRDGRIIAAQSF
                     KQVEPAASVSGAQAAAALDKALSAVMARIVAFTAAKL"
     gene            complement(3165..3530)
                     /locus_tag="VKU03_11445"
     CDS             complement(3165..3530)
                     /locus_tag="VKU03_11445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71923.1"
                     /translation="MSLSALSRFKRDHLLFGRRGVKRTSEWAASNAPSWPSIQPWISA
                     PSIASCFVTLGVAAFFLASFSHMPGEFRGWRASQSSHAAAEAKARIGSPDRSSRAACG
                     AARRRPGFGDAARLTASPR"
     gene            3630..3848
                     /locus_tag="VKU03_11450"
     CDS             3630..3848
                     /locus_tag="VKU03_11450"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HLJ71924.1"
                     /translation="MPEDLRRALAASPAARRFFDRLDSANRYAILYRLHHAKDGAPRA
                     ARLAKFIAMLEAGETFHPLRAKRGRKSD"
     gene            complement(3886..>4123)
                     /locus_tag="VKU03_11455"
     CDS             complement(3886..>4123)
                     /locus_tag="VKU03_11455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLJ71925.1"
                     /translation="AVMIDYYRAFRTFMAATRDPAETIAFKLKAGEMVVFDNRRVLHG
                     REAFNPATGHRLLRGFYIDRGEIDSRIRVLSRTA"
BASE COUNT          710 a         1385 c         1367 g          661 t
ORIGIN      
        1 caggaaacgg cgcgcgcgag gcggtcatcg ccgtgcgcga cctgcacgtc gaggtcggcg
       61 agcagaaggt gctcaagggg ctcacgctcg acgtctaccg gggcgagatc ctcggcttcg
      121 tcggcgcctc cggggcgggc aagtcggtgc tgatgcgcac catcatcggc ctgctgccga
      181 aacgccgcgg cacgatcagc gtgctcggcg tcgaccgcga ccgggcgagc gaggcggagc
      241 gccaggcggt cgagcggcgc tggggcatcc tgttccagca gggcgccttg ttctccaacc
      301 tcaccgtcaa gcagaacgtc caggcgccga tgcgcgagca tctcggcatt tccaagcgcc
      361 tgcgcgacga attggcgatg ctcaagatcg ccctggtcgg cctgtcgccc gacgcggcgg
      421 agaaatatcc gtccgagctc tccggcggca tgatcaagcg cgccgccttg gcccgcgcct
      481 tggcgctcga tccggacatt cttttcctcg acgagccgac atccggcctt gacccgatcg
      541 gcgccggcga atttgacgag ctcctggcga cattgcagaa aactttgggt ctttcggtct
      601 tcatggtgac gcatgatctc gacagtctct atgcggtctg cgatcgtgtc gccgcgctcg
      661 gccagggtcg ggttattgcg gccggaccga tcgccgatct cctcaattcg gacgatccct
      721 ggctgaaagc ctatttccat gggaaaaggg gcgccggccg cgaggcggcg caggcgcgcc
      781 gcggcgtgga gtagaaggtg aaatgaggct gtgtcgactg atcgtcattg cgagcgaagc
      841 aaagcaatcc attcttaggg cgccgcttta ccaacggatt gcttcgccgc gctcgcaatg
      901 acgggacttg tatggccagc cgccaaatgt gacgagacgg aatggagacg cgcgcaaatt
      961 atgtcctgat cggcgtgttc accttggcgg tgatcgcggg cggattcctg ttcgtgatgt
     1021 ggttctcggg actcggcagg ggcgcccaga acaagaccta tgaggtcgtc ttcaccgggt
     1081 cggtctccgg cctgtcgcgc ggcagtctgg tcagcttcaa cggcctcaga gtcggcgagg
     1141 tgacgcagat cagcttcttt ccgaacgatc cgagccgggt cgcggcgatg atcaacgttg
     1201 tcgccaacac gccggtcaag accgacacca aggcgcggct cgaatcgcaa ggcctgaccg
     1261 gcgttgcgac tttggcgctg accggcgaat cgagctccgc cccgccgctt gagccgggtc
     1321 ccggcggcgc gccgccggtc atcaaggccg aacggtcgga ctttcagaac ctgctcgaga
     1381 cggtgcagcg gatttcggga aaggccgacg aggtcttgac caaagccaac aagctgctcg
     1441 atgagaacgg cccggcggtc ggcgacgcgc tgcgcaatgt cgatgagttt tcgaaagcgc
     1501 tcgccgacaa ttcagccggc gtgaagacgt tcctcgccag cgtcggcgat ctcggccaga
     1561 agctcgcgcc gctttcgtcg aagttggaga ccttgtcgag cgatgtcgat gcggcggtca
     1621 aggcggtcga tccggccaag gtgaaaaaca ttctcgccaa tgccgacgat ttctcggcgg
     1681 cgctggcgcg caacaaggac aatatcgata cgaccctcgc cggcgccgcc gccatcgcca
     1741 agcgccttga cggaacgtcg cagaagctcg atgcgacgct cgacaatctc gccgccttct
     1801 ccgccacgct cgccggcaac aaggccaata tcgacagcct gctcgccgac tcggcggcgc
     1861 tcgccaagcg ccttggcggg acctccgaca agctcgacgc ggcgctcgac caagtcaccg
     1921 gcctggtcaa atccgtcgac cgcgacaagg tcgcgggcat tgtcgacaac gtctccggct
     1981 tcagccagac cctgtcgcgc aatcagggca atatcgacag cctgctcgcc gactcggcgg
     2041 ctctggccaa gcgcctcaac ggcacgtcgc aaaagctgga caatgcgctc gaccaggtca
     2101 gcgatctcgc caaggcgttc gaccgcgcca agatcgccag cgccgtcgac aacgtcgccg
     2161 agttcaccga cgcgctgaag cagaaccgcg gcaatgtcga ccatatgttg aaggacgccg
     2221 ccgagctcgc cgccaagctc aacggctcgg ccgaccagat cgacggcctg ttgcgcagcc
     2281 tgcaggggct gatcggctcg ccggactcca agggcgcgat cgcccaagtc ggcgacgccg
     2341 cccgcgcgat ccgccagctc gccgaaaatc tcgatcagcg cgtgaaggaa atttccgtcg
     2401 ggctgacgcg cttctcggga tcgggcctgc gcgaatacga ggcgctggcg atcgacggac
     2461 ggcggacggt caacgacatc gaccgtttcg tgcactcgct gagcaacaat ccaagccagg
     2521 tgatattcgg ggcgaagccc gctttgcccg attatcacgc gggtcaatag aggcgggggc
     2581 gcggcggggc atgttcggac cggtcggccg cgctcggcgc ctcgtcgcgg cggcgctcgc
     2641 tcttccctta gtgcttgggc tcgccgcctg cggcggcggc gcgtcgttgc cgagcttcga
     2701 cctctcggcg acgtcggcgc cgagcgaacg cccgttgcgc gcttcgctga tcgtacgcca
     2761 gccgctctct cccctcgcct tcgattcgcg gcgcatcgtc gtgcggaccg ggccggacgc
     2821 gctcgcctat ctcgccgacg cgcaatggtc ggatcgcctg ccggtcctcg tccaatcccg
     2881 cttggccgag acctttcaga acgcccattt gtttcgctcc gtcgagcgcg ccagcaagcc
     2941 ggcggattac gcgctcgaac tggaaatccg gcatttcgag atcgacgcgc caagccgcgc
     3001 cggaatcgtc gagatcgccg ccaagatcgt cagcggccgc gacgggcgga tcatcgcggc
     3061 gcaaagcttc aaacaggtgg agccggccgc gagcgtgagc ggcgcgcaag ccgcagcggc
     3121 gctcgataag gcgctctcgg cggtgatggc gcggatcgtc gccttcaccg cggcgaagct
     3181 gtgagccgag ccgcgtcgcc gaaaccgggc cgccgccgcg ccgcgccgca agccgcgcgc
     3241 gaagagcggt ccggactgcc gatcctggcc ttcgcctcgg ccgcggcgtg ggaggattgg
     3301 ctcgcccgcc agccgcgaaa ctcgcccggc atatggctga agctcgccaa gaagaacgcc
     3361 gcaacgccga gcgtcacgaa gcaggaggcg atcgacggcg cgctgatcca tggctggatc
     3421 gacggccagc ttggcgcatt cgacgccgcc cattcgctcg tccgcttcac gccgcgccgg
     3481 ccgaagagca gatggtcgcg cttgaatcgc gacagggcgg aaaggctcat cgccgcgggc
     3541 cgtatggcgg cggcgggctc gaggaaatcg aggccgcgag ggcggacggc cgctgggatg
     3601 cggcctatca gccgcagagc gcttccggcg tgccggaaga ccttcgccga gctctcgccg
     3661 cttcgccggc ggcgcgccgg tttttcgaca ggctcgacag cgcgaaccgc tatgcgatcc
     3721 tctatcgcct gcatcatgcc aaggacggcg cgccgcgcgc ggcgcgcctc gccaagttca
     3781 tcgccatgct cgaggccggc gaaacgtttc acccgctcag ggccaagcgc ggccgaaagt
     3841 cggattagat tgacgcggcg caggcggcgc cgcgccagtc atgggctaag cggtccgcga
     3901 caggacccgg attcggctgt cgatctcgcc gcggtcgata tagaagccgc gcagcaggcg
     3961 atgcccggtc gcaggattga acgcctcgcg cccatgcagc acgcggcgat tgtcgaacac
     4021 gaccatttcc ccggccttga gcttgaaggc gatggtctcg gccggatcgc gcgtcgccgc
     4081 catgaacgtt cggaacgcgc ggtaatagtc gatcatgacg gcg
//
LOCUS       DATGJW010000378         2477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1209546, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000378 DATGJW010000000
VERSION     DATGJW010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2477
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1209546"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1435)
                     /locus_tag="VKU03_11460"
     CDS             complement(<1..1435)
                     /locus_tag="VKU03_11460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018688359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HLJ71926.1"
                     /translation="MLSDHLVVFTPSGKRERLPEGASVLEAARRLGVDLDSVCGGRGI
                     CGRCQIEVASGHFAKHGVVSAPDHVTAWNGVEQRYVEKRGPLAPGRRLGCQAKICGDL
                     VVDVPPDSQVHRQVVRKRAESHPIEVDPVVRLYYVEVREPDMHDPSSDLRRLQSALKS
                     QWSLGETFADLAVLRGLQKTLRAGEWKATVALRKGRDIVAVYPGFRDRALGIAFDIGS
                     TTIAAHLCDLASGDVLASSGAMNPQIRFGEDLMSRVSYVMMNPGGDKELTESVRAALD
                     ALIGDVVRQPGAERDDVVEITLVGNPIMHHLALGLDPTELGGAPFALTIDGAQEVRAA
                     ELGLAIAPGALVYCLPCIAGHVGADTAGMVLAEGPYLRDDLTLLVDVGTNAEIVFGNR
                     ARLLACSSPTGPAFEGAQISCGQRAAPGAIERVRIDRLTLEPRFKVIGCDLWSNDEGF
                     AEATASLGITGICGSGIIEIIAEMYLAG"
     gene            complement(1436..2350)
                     /locus_tag="VKU03_11465"
     CDS             complement(1436..2350)
                     /locus_tag="VKU03_11465"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HLJ71927.1"
                     /translation="MTETRLCSDKREVVIGFDRRFVMIGERINPTGRKLLAAEMAAGD
                     YSRVVADAIAQVEAGAQMLDVNAGVPLADEPKILADCIKLVQETVPVPLSIDSSIVEA
                     LEAGLAVYKGRPLVNSVTGEEERLERVLPLIKKYDAAVIAISNDETGISEDPDVRYAV
                     AKKIVERAQDFGIKREDVVVDPLVMPVGAINDAGAKLTYLLRRLREELKVNTSCGASN
                     FSFGLPNRRGLGASFLPMMIGAGMTSAIMNPLHEEDRQAILGADVVMGHDPNCAAWIR
                     KYREPPAEGEAAAGEGRGRREGRRRARE"
BASE COUNT          404 a          809 c          862 g          402 t
ORIGIN      
        1 tcccggcgag gtacatttcg gcgatgattt cgatgatgcc ggagccgcaa atgccggtga
       61 ttccgagcga cgcggtcgcc tcggcgaagc cttcgtcgtt cgaccagagg tcgcagccga
      121 tcaccttgaa gcgcggctcg agcgttaagc ggtcgatgcg cacgcgctcg atcgcgccgg
      181 gcgcggcgcg ctggccgcag gaaatctgcg cgccttcgaa agccgggccg gtcggcgagg
      241 agcaggcgag taggcgggcg cgattgccga agacgatctc ggcattggtg ccgacatcga
      301 cgagcagcgt gagatcgtcc cggaggtacg gaccttccgc caggaccatg ccggccgtat
      361 cggcgccgac atggccggcg atgcaaggca ggcaatagac caaggcgccg ggcgcgatgg
      421 cgaggccgag ctcggccgcc cggacctcct gcgccccgtc gatggtcagc gcgaaggggg
      481 cgccgcccag ctcggtcgga tcgaggccga gggcgagatg atgcatgatc ggattgccga
      541 cgagggtgat ctcgacgacg tcgtcgcgct cggcgcccgg ttgcctgacg acatcgccga
      601 tcagcgcgtc gagcgcggcg cgcacgctct cggtcagctc cttgtcgccg cctggattca
      661 tcatcacata ggaaacccgg ctcatcaggt cttcgccgaa gcggatctgc gggttcatcg
      721 cgccggaaga ggcgaggacg tcgccgctcg ccaggtcgca gagatgggcg gcgatcgtcg
      781 tcgagccgat gtcgaaggcg attccgagcg cgcggtcgcg gaagccggga tagaccgcga
      841 cgatgtcgcg gcccttgcgc agagcgacgg tcgccttcca ttcgccggcg cggagcgtct
      901 tctgcaggcc gcggagcacg gcaagatcgg cgaaggtctc gccgagcgac cactggctct
      961 tgagcgccga ttgcagccgg cgcaggtcgc tcgacggatc gtgcatgtcg ggctcgcgca
     1021 cctcgacata gtacaaacgc acgaccggat cgacttcgat cggatgggat tcggcgcgct
     1081 tgcgcaccac ctggcgatga acctgcgaat ccggcggcac gtcgacgacg agatcgccgc
     1141 agattttcgc ctggcagccg aggcgccggc cgggcgcgag cggcccgcgc ttctcgacat
     1201 agcgctgctc gacgccgttc caggccgtga cgtgatcggg cgccgagacg acgccgtgct
     1261 tggcgaaatg gcccgacgcg acctcgatct ggcagcggcc gcaaatgccg cggccgccgc
     1321 agaccgaatc gagatcgacg ccgaggcggc gggccgcttc gagcacgctc gcgccttccg
     1381 gcaggcgctc gcgcttgccc gacggcgtga agacaacgag atgatcgctc aacatctact
     1441 cccgggcgcg gcggcgccct tcgcggcggc cgcggccttc gccggcggcg gcctcgccct
     1501 ccgccggcgg ctcgcgatac tttctgatcc aggcggcgca attcgggtcg tggcccatca
     1561 ccacatcagc gccgaggatc gcctgcctgt cctcctcatg aagcgggttc atgatcgccg
     1621 aggtcatccc ggcgccgatc atcatcggca ggaacgaggc gccgaggccg cgccggttgg
     1681 gcaggccgaa ggagaaattg gaggcgccgc aggacgtgtt gaccttcagc tcctcgcgca
     1741 gccgtcgcaa aagataggtg agcttcgcgc cggcgtcgtt gatcgcgccg accggcatga
     1801 ccagcggatc gacgaccacg tcctcgcgct tgatgccgaa atcctgcgcc cgctcgacga
     1861 ttttcttggc gacagcgtag cgtacgtccg ggtcttcgga aatgccggtc tcgtcgttcg
     1921 aaatggcgat caccgcggcg tcgtatttct tgatcagcgg caggacgcgc tcgagccgct
     1981 cttcttcgcc ggtgaccgaa ttgacgagcg ggcgcccctt atagacggcg agcccggctt
     2041 cgagcgcctc gacgatcgag gaatcgatcg acagcggcac gggcacggtc tcctgcacca
     2101 gcttgatgca atcggcgaga attttcggtt cgtcggcgag cggcacgccg gcattgacgt
     2161 cgagcatctg cgcgccggct tcgacttgcg cgatcgcgtc ggcgacgacg cggctgtaat
     2221 cgccggccgc catttccgcg gcgagcaatt tgcgccccgt cggattgatg cgctcgccga
     2281 tcatgacgaa gcggcggtcg aagccgataa cgacttcacg tttgtcggag cataggcgcg
     2341 tttcagtcat gggcggtcct catgtggctt tcctcccccg cgaagcgggg gtacctgttt
     2401 agcgccctaa gcgctttctg ggacgagggg aagccttggg gcgaggaaat cggcgaaggg
     2461 gacgccggtt tggcggc
//
LOCUS       DATGJW010000379         5681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_72214, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000379 DATGJW010000000
VERSION     DATGJW010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5681
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72214"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..589)
                     /locus_tag="VKU03_11470"
     CDS             complement(<1..589)
                     /locus_tag="VKU03_11470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease activity regulator RraA"
                     /protein_id="HLJ71928.1"
                     /translation="MPLDAAIAAELAAISTATLTTVLLKKGLRRVAMRGPKPFGAASG
                     GRVAGRAFTLRFAPAREDLATPESWASPRSTRAAIEAMAPGDIAVVDAMGVTTAGIFG
                     DILCARMKKRGLAALVTDGAVRDGSGVAATGLPVWCAGIAAPPSVAELTFVGWGEPIG
                     CGGVAVMPGDVVVADGDGAVLIPDALLAAVLAEARE"
     gene            complement(666..1037)
                     /locus_tag="VKU03_11475"
     CDS             complement(666..1037)
                     /locus_tag="VKU03_11475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ71929.1"
                     /translation="MSEEALRDRRILLVEDEFLLAQNLSDEFKAIGAAVLGPVASLER
                     ALALIEQEQAIDVAVLDLNLGGVMAYPVADALLARRIPFVFTTGYEDASIRPRYPAAR
                     TCEKPFAFSALAKALADALAA"
     gene            complement(1030..2502)
                     /locus_tag="VKU03_11480"
     CDS             complement(1030..2502)
                     /locus_tag="VKU03_11480"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLJ71930.1"
                     /translation="MTDLPPFPASVMAGLRNSEERLRLALTAAAMGTFVWRIGEDRSE
                     LDAQMLALLGRPADDAIIANAALAALIHPADRQRFAEAVARALAAGPGGELSEDVRIQ
                     LAGGATRWLAVTAQVYFEGEPRRATRMVGAAADITERKKAEAVLRESEERARVALEAG
                     RMGTWRYDLASGAQQWDLRQYALFGLDPGVTPDRALFLSLVHPDDLELVAFDAAGLPP
                     PGSFLDSEFRIVRPDGETRWIAAHALARYDEDGKPVEMIGVNLDVTETKRAVEALRDS
                     EERLHVLVGELQHRTRNLIGVIRALADRTLRSSGALEDFKSKFQERLAALGRVQGLLS
                     RVKDNERVTFDELLASELAAQTSSGGDRGRVVLDGPQGVRLRTGTVQTFALALHELTT
                     NAVKYGAFKQPHGRLKVSWRCELGKGGKPWLHVDWKESGVVMPADRRAQGGGQGRELI
                     EQALPYQFGALTRFALEEDGVRCTIALPMSESQADGAGHE"
     gene            complement(2596..3033)
                     /locus_tag="VKU03_11485"
     CDS             complement(2596..3033)
                     /locus_tag="VKU03_11485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1489 domain-containing protein"
                     /protein_id="HLJ71931.1"
                     /translation="MTLHLIKLCVGADSVEDLQSWIDERMAMKKKRGEKAEHKHITRM
                     VPKRVDALLDGGSLYWVIKGQIAARQGLAAIRPFIDGDGIGRCELVLEPVARPVLPRP
                     CRPFQGWRYFDVKDAPPDLTPRLGDVAHMPETMRRELSELGLI"
     gene            complement(3105..3545)
                     /locus_tag="VKU03_11490"
     CDS             complement(3105..3545)
                     /locus_tag="VKU03_11490"
                     /inference="COORDINATES: protein motif:HMM:NF013939.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate mutase"
                     /protein_id="HLJ71932.1"
                     /translation="MTKRWSAAFALLTGAALLAAGASGMAEDAPHAFWGKPSVDGGKC
                     CNSLAEVRENIDRINRDLVRLMAERQKYVAEAGRFKANPAAVSAPARVEQIIENVKAL
                     ARQEGLSEAVAEKTFRAMISGFEDYEREVWAHKNAAGGAPAKTP"
     gene            complement(3547..5499)
                     /locus_tag="VKU03_11495"
     CDS             complement(3547..5499)
                     /locus_tag="VKU03_11495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin carboxylase N-terminal domain-containing
                     protein"
                     /protein_id="HLJ71933.1"
                     /translation="MFDSVLIANRGEIACRVIRTAKRLGVRTIAIYSAADRGAPFMRL
                     ADEAHEIGPAPARESYLNGEKIVALAKKIGAAAIHPGYGFLSENAEFAESCGAAGIVF
                     IGPPPSAIRAMGLKSAAKALMQKAGVPVTPGYHGDNQSPKFLREKAYEIGYPVLIKAI
                     AGGGGKGMRRVDAHVDFEAALESAAREAENAFGDARVLVEKYVARPRHIEMQIFGDAH
                     GEVVHLFERDCSLQRRHQKVIEESPAPDLSEETRAAMAAAAVAAGRAVGYVGAGTVEF
                     IADSSRGLRPDGFYFMEMNTRLQVEHPVTEAITGLDLVEWQFRVAAGETLPLSQKDIR
                     RSGAAVEARLYAEDPEHGFLPSSGKIYALRLPAGQGIRVDSGVEESGEVTPFYDPMIA
                     KIIAHGASRDEALDRLSRALGETLVAGPRVNAAFLKALCEAKEFRAGAFDTGFIDANL
                     ERLGAAPRALDAGAALAGLDRLLARRSARFAAADSPRDPWSVADFFELAGKRVVGVDA
                     LVDGKPVHFVETTANGTRRLALAGGASPDEPSPPTIIEAEGRVFALRDGRQTEVRLVD
                     PLASGAEASAEESGAVEAPMHGRLLALYVKDGDVVEKGARLAIVEAMKMEHALNAPRA
                     GRVAGIAHAPGAQVAQGARLMTIEEP"
BASE COUNT          917 a         1995 c         1887 g          882 t
ORIGIN      
        1 gctcgcgcgc ctcggcgaga acggcggcga gcagcgcgtc cggaatcagc actgcgccgt
       61 cgccgtcggc gaccaccacg tcgccgggca tcaccgcgac gccgccgcag ccgatcggct
      121 cgccccagcc gacaaaagtc agttccgcca ccgaaggcgg cgctgcgatt ccggcgcacc
      181 agaccggcag gccggtcgcc gcgacgcccg agccgtcgcg caccgcgccg tcggtgacga
      241 gggcggcaag gccgcgcttc ttcattcgcg cgcagagaat gtcgccgaat atgccggcgg
      301 tcgtcacgcc cattgcgtcg accacggcga tgtcgcccgg cgccatcgct tcgatcgccg
      361 cccgggtcga gcgcggcgaa gcccaggatt cgggcgtcgc caaatcctcc cgcgccggcg
      421 cgaagcgcag ggtgaaggcg cggccggcga cgcggccgcc cgaagccgcc ccgaacggct
      481 tcggcccgcg catcgcgacg cgccgcaaac ctttcttcaa caggacggtg gtcaaggtcg
      541 ccgtcgagat ggcggcgagt tcggcggcga ttgcggcgtc gagcggcatg acgagctccg
      601 gtggagccgc gacaatggcg cgggcgccgg ccggcgtcca gcgatgcgtt cccccgcgcg
      661 cgccgtcagg ccgccagagc gtccgcgagg gctttggcga gcgcgctgaa ggcgaacggc
      721 ttttcgcagg tccgggccgc cggatagcgc ggacggatcg aggcgtcctc atagccggtg
      781 gtgaagacga aagggatgcg ccgggccagc agcgcgtctg cgaccggata ggccatgacc
      841 ccgccaagat tgagatcgag gacggcgacg tcgatcgcct gctcctgctc gatcaaagcc
      901 aaggcgcgct ccagcgacgc aaccggaccg agaaccgccg cgccgatcgc cttgaactcg
      961 tcgctcagat tttgcgccag cagaaattcg tcctcgacga ggaggatacg gcgatcgcgc
     1021 aaggcctctt cactcatgtc ccgccccgtc ggcctggctt tccgacatcg gcagcgcgat
     1081 cgtgcaacgg acgccgtctt cttcgagggc gaaacgggtg agcgcgccga actgataggg
     1141 cagcgcctgc tcgatcagct cgcgcccctg gccgccgccc tgcgcccgcc gatcggccgg
     1201 catgacgacg ccgctttcct tccaatcgac gtgaagccat ggcttgcccc ccttgcccaa
     1261 ttcgcagcgc cagctcactt taagccggcc atgcggctgc ttgaaggcgc cgtatttgac
     1321 ggcgttggtc gtcagctcgt gcagagccag ggcgaacgtc tgcaccgtgc cggtgcgcag
     1381 gcgcacgcct tgcggtccgt cgagaacgac gcggccgcgg tcgccgccgc tgctggtttg
     1441 cgccgccagt tcgctcgcca gcagctcgtc gaacgtgacc ctctcattgt ccttgacgcg
     1501 ggagagcagc ccctggacgc ggccgagcgc cgccaggcgc tcctggaact tggacttgaa
     1561 gtcttcgagc gcgccgctcg accgcaaagt acgatcggcg agagcgcgga tcacgccgat
     1621 caagttgcgg gtgcgatgct gcagctcgcc gaccagcaca tgcagccgct cctcgctgtc
     1681 gcgcagggct tcgaccgccc gcttggtttc ggtcacgtcg agattgacgc cgatcatttc
     1741 aaccggcttg ccgtcctcgt cgtagcgcgc cagcgcatgc gccgcgatcc agcgcgtttc
     1801 gccgtccggc cggacgatgc ggaattcgct gtcgaggaac gagccgggcg gcggcaagcc
     1861 ggcggcgtcg aaggcgacga gttcgagatc gtccggatgg acgagcgata gaaagagcgc
     1921 gcggtcgggc gtcacgcccg gatcgagccc gaacagcgca tattgccgca aatcccattg
     1981 ctgggcgccg ctggcgagat cgtagcgcca cgtgcccatg cggccggctt cgagcgcgac
     2041 ccgcgcccgc tcttcgcttt cgcgcagcac ggcctccgct ttcttgcgct cggtaatgtc
     2101 ggccgccgcc ccgaccatgc gcgtggcccg gcgcggctcg ccctcgaaat agacttgggc
     2161 ggtgacggcg agccaccgcg tcgcgccgcc agccagttgg atgcggacgt cctcgctcaa
     2221 ctcgccgccc ggcccggcgg cgagggcgcg cgccaccgcc tcggcgaagc gctggcgatc
     2281 ggctggatgg atcaaggccg ccaaggcggc gttggcgatg attgcgtcgt ccgccggccg
     2341 gccgagcagc gccagcattt gcgcatcgag ctcgctgcgg tcttcgccga tccgccaaac
     2401 gaacgtcccc atcgccgcgg cggtcagagc gaggcgcagc cgctcctcgc tgttgcgcag
     2461 gcccgccatg acggaggccg gaaaaggcgg caggtcggtc atcgcgtcgg aaccgcggcg
     2521 gggcgtttaa gccgatgaga atagccgcgg agccggaaaa ggccatccgc ggcgatgcgt
     2581 tccagcggaa gcgcttcaga taagcccgag ctcgctcagt tcgcgccgca tggtctcggg
     2641 catatgggcg acgtcgccga gccgcggcgt gagatcgggc ggcgcgtcct tgacgtcgaa
     2701 ataacgccag ccctggaaag gccggcaggg ccgcggcaga accggccgcg ccaccggctc
     2761 cagaaccagc tcgcaccggc cgatcccgtc gccgtcgata aaggggcgga tggcggccaa
     2821 accctgccgc gccgcgatct ggcctttgat cacccaatag agcgagccgc cgtcgagcag
     2881 cgcgtcgacg cgcttcggga ccatgcgggt gatgtgcttg tgctccgcct tctcgccgcg
     2941 ctttttcttc atggccatgc gctcgtcgat ccaggattgc aaatcctcga cggaatcggc
     3001 gccgacgcag agcttgatca aatgcagggt catgacgggc gattatggcg ccggccggtc
     3061 gcctctagaa gggccgccaa gcgccgcgct gtggatggcg aaagtcacgg cgtcttggcc
     3121 ggggcgccgc ccgccgcgtt cttatgcgcc cagacctcgc gctcgtaatc ctcgaagcct
     3181 gagatcatcg cgcggaacgt cttttcggcg acggcttcgg agaggccctc ttgccgcgcc
     3241 aaggccttga cgttctcgat gatttgttcg acccgcgccg gcgcgctgac ggcggccgga
     3301 tttgctttga accggcccgc ttcggcgaca tatttctggc gctcggccat caggcggacg
     3361 agatcgcggt taatgcggtc gatgttctcg cgcacttcgg cgagcgagtt gcagcatttg
     3421 ccgccatcga ccgacggctt gccccaaaaa gcgtgcggcg cgtcctcggc catgccgctc
     3481 gcgccggccg ccagcaaggc ggcgccggtc aacagagcga aagcggcgct ccatcgtttc
     3541 gtcatatcaa ggctcctcga tcgtcatcaa tcgcgcgccc tgcgccactt gcgcgccggg
     3601 ggcgtgagcg atgccggcga cgcggccggc gcgcggcgca ttgagcgcat gctccatctt
     3661 catcgcctcg acaatggcga ggcgcgcgcc tttttcgacg acgtcgccgt ccttgacgta
     3721 gagcgcgagc agccggccat gcatcggcgc ttcgaccgcg cccgattctt ccgccgaggc
     3781 ttcggccccg gaagccagcg gatcgacgag cctgacctcg gtctgccttc cgtcgcgcag
     3841 cgcgaagacg cgtccctccg cttcgatgat cgtcggcggc gacggctcgt cgggcgacgc
     3901 gccgcccgcc aaagcaagcc gcctcgtccc attggcggtt gtctcgacga aatggaccgg
     3961 cttgccgtcg accagggcgt cgacgccgac aacccgcttg ccggccagtt cgaaaaaatc
     4021 ggcgaccgac cagggatcgc gaggcgagtc ggccgcagcg aagcgcgcgc tgcggcgcgc
     4081 gagcaggcga tcgagcccgg ccaaagcggc gcccgcatcc aaagcccgcg gcgcagcgcc
     4141 gagccgctcg agattggcgt cgatgaagcc ggtatcgaaa gcgccggcgc gaaattcctt
     4201 ggcctcacac agcgccttca gaaaagcggc gttgacgcgc gggcccgcga ccagggtttc
     4261 gcccagcgcc cttgatagcc ggtcgagcgc ctcgtcgcgg ctcgcgccat gggcgatgat
     4321 cttggcgatc atcggatcgt agaacggcgt cacctcgccg ctttcctcga cgccggaatc
     4381 aacgcgaatc ccctgccccg ccggcagccg caacgcatag attttgccgg aggacggcag
     4441 gaagccatgt tccggatctt ccgcatagag ccgcgcctcg accgccgcgc cgcttcggcg
     4501 gatgtctttt tgcgagagcg gcaacgtctc gccggcggcg acgcgaaatt gccactcgac
     4561 gagatcgagc ccggtaatcg cctcggtaac cgggtgctcg acctgcagcc tcgtgttcat
     4621 ctccatgaaa tagaagccgt ccggccgcag gccgcgcgag gaatcggcga tgaactcgac
     4681 cgtgccggcg ccgacataac cgaccgccct gccggccgcg accgccgccg ccgccatggc
     4741 ggcgcgggtc tcctcgctta aatccggcgc cgggctctcc tcgattactt tctgatggcg
     4801 gcgctgcaga gaacaatcgc gctcgaacag atggacgacc tcgccatgcg cgtcgccgaa
     4861 aatctgcatc tcgatatgac gcggccgcgc gacatacttt tcgaccagca cccgcgcgtc
     4921 gccgaacgcg ttctcagcct cgcgcgccgc gctttcgagc gccgcttcga aatcgacatg
     4981 ggcgtcgacg cggcgcatgc ccttgccgcc gccgccggca atcgccttga tcaggaccgg
     5041 atagccgatc tcataggcct tctctctgag gaatttcggg ctctgattgt cgccgtgata
     5101 gcccggcgtc accggtacgc cggctttctg catcagcgcc ttggcggcgc tcttcaagcc
     5161 catggcgcgg atcgccgaag ggggcgggcc gataaagaca atccccgccg cgccgcagct
     5221 ttccgcgaac tcggcgttct cggagagaaa gccatagccg ggatggatcg cggcggcgcc
     5281 gatctttttg gccagggcga cgattttctc gccgttcaga taggattcgc gcgccggcgc
     5341 cggcccgatt tcatgcgcct cgtcggcgag gcgcatgaac ggcgcgccgc gatcggccgc
     5401 ggaatagatc gcgatcgtgc gaacgccaag cctcttggcc gtgcggatga cccggcaggc
     5461 gatctcgccg cgattggcga tgaggacgga atcgaacaag ggcaactcca aagggggtcg
     5521 ccgaacctag cgcgtcctcg gccctcgtca aacccgatgg cctttcgagc aaacgcaacc
     5581 gcgtgggaga ggagccgccg gtcgtggcgg catgaagcgc tagataagcc agcgacgtac
     5641 cgatccgtct cgacagactt tcctcgccgc ggcggcaaaa c
//
LOCUS       DATGJW010000380         2491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_482623, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000380 DATGJW010000000
VERSION     DATGJW010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2491
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_482623"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..576)
                     /locus_tag="VKU03_11500"
     CDS             complement(<1..576)
                     /locus_tag="VKU03_11500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010970560.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF993 family protein"
                     /protein_id="HLJ71934.1"
                     /translation="MVKIHLPRADRSVELFELSPPSPFPRKQSQPFPRIGYAAAHVVA
                     DPLGESGAAIDWDRTLAFRHHLWDLGFKIAEAMDTAQRGMGLDWAGAQELIRRALAEA
                     KARPGADLACGAGTDHLDPSAARSLADVVAAYEEQVGFVEGQGGRAVMMASRALARLA
                     RGPDDYAAVYDRILTQARRPVILHWLGEMFDP"
     gene            808..1857
                     /locus_tag="VKU03_11505"
     CDS             808..1857
                     /locus_tag="VKU03_11505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007598753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HLJ71935.1"
                     /translation="MGDHKPARRSTTMIDIARAAGVSKSTVSLVLKGSDLVKADTRQR
                     VEAAIERLGYVYNRGAANLRASSSSFVGMVISDLTNPFFAELAVGIENALYQLGFVPI
                     LANTNEDVERQAYVLRSLREHNVAGIIMSPARGADAWALANQLPATMPTIITMRRIPG
                     SPFPYIGPDNRAGSREAVAHLLRLGHRRIGYLGGDEKITTQQERAAGWQDALAAAGIP
                     YDPDLMFPAPPTRDGGRDAMERALAMARPPTAVFCYNDIVAMGATRALSMRGLAPGRD
                     VAVIGFDDIAEAEHNAPPLTTVNADTREMGARCAESLIRLIRGEDPAGLSFAGRTRLI
                     VRESCGAARLERKAS"
     gene            1854..>2491
                     /locus_tag="VKU03_11510"
     CDS             1854..>2491
                     /locus_tag="VKU03_11510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019861069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ71936.1"
                     /translation="MTQKVRWGLIGATTIAREWMIAAIRAVGGEIAGVMSSNAERGQK
                     YAAEFGVPLATTSLDKLFEACDAVYVATTNDLHYAQVLAAAKAKKHVLCEKPLALTLP
                     EAREMVAACKAAGVVMGTNHHLRNAATHRAMREAIAAGRIGRPLFARVCHAVYLPEHL
                     QGWRIKDPKTGPGVVMDITVHDSDTLRFVLGDEPEAATGMVQRAGMGAGGIE"
BASE COUNT          450 a          829 c          826 g          386 t
ORIGIN      
        1 cggatcgaac atctcgccaa gccaatggag gatgaccggc cggcgcgcct gggtgagtat
       61 gcggtcatag acggcggcgt agtcgtccgg tccgcgcgcg aggcgcgcca gggctcggct
      121 cgccatcatg accgcgcggc cgccctggcc ttcgacaaag ccgacctgct cctcataggc
      181 cgcaacgacg tcggcgagcg agcgcgccgc tgaaggatcg agatgatccg tgccggcgcc
      241 gcacgcgaga tcggcgccgg gccgcgcctt ggcttcggcg agcgcgcgcc tgatcagttc
      301 ttgcgccccg gcccaatcga ggcccatgcc gcgctgggcg gtgtccatcg cctcggcgat
      361 cttgaagccg agatcccaga gatgatgtct gaaagctaag gtacgatccc agtcgatcgc
      421 cgcgccggat tcgccgagcg gatcggcgac gacatgggcg gcggcgtagc cgatgcgcgg
      481 aaagggctga ctttgttttc gcgggaaagg cgaaggcggc gagagttcga aaagctcgac
      541 ggacctatcc gctctgggca aatgaatttt gaccatcgac gccttcccct gtctcgcgcg
      601 ccgcccgatc ggctccgcgc aaagcgccgg cggaccaatt cactagaacg ttccaaagtc
      661 aaggcatcaa tcagtagaga gaaaaagatg ttataattca atattgcgat gagatgcttg
      721 acaaagataa acctccacaa taggaacgtt ccaatcacgg cgctgcattg accgtttgaa
      781 gcgcgccgga tcgggagggg catccccttg ggcgaccaca agccggcgcg acgctcgacg
      841 acgatgatcg acatcgcccg cgccgccggc gtgtcgaaat ccaccgtttc cctggtccta
      901 aaaggctcgg acctcgtcaa ggccgatacg cgtcagcgcg tcgaggcggc gatcgagcgc
      961 cttggctatg tctacaatcg gggcgccgcc aatctgcggg cttcctcctc aagctttgtc
     1021 ggcatggtca tcagcgacct gaccaatccg ttcttcgcgg aattggcggt cggcatcgag
     1081 aacgcgcttt atcagctcgg cttcgtgccg attctcgcca atacgaacga ggacgtcgag
     1141 cgccaagcct acgtgctgcg ctcgctgcgc gagcacaatg tcgccggcat tatcatgagc
     1201 ccggcgcgcg gcgccgacgc ctgggcgctc gccaatcaat tgccggcgac catgccgacc
     1261 atcatcacga tgcggcgcat cccgggcagc ccctttccct atatcggtcc cgacaatcgc
     1321 gccggctcgc gcgaagcggt ggcgcatctc ctgcggctcg gccatcgccg catcggctat
     1381 ctcggcggcg acgagaagat cacgacccag caagagcgcg ccgccggctg gcaggacgcg
     1441 ctcgcggccg ccggcattcc gtacgatccg gacctgatgt tcccggcgcc gccgacccgc
     1501 gacggcggcc gggacgcgat ggagcgcgcg ctcgccatgg cgcggccgcc gaccgcggtc
     1561 ttttgctata acgacatcgt cgccatgggc gcgacccgcg cgctgtcgat gcgcggcctg
     1621 gcgccgggcc gcgacgtggc ggtgatcggc ttcgacgaca tcgccgaggc cgagcacaat
     1681 gcgccgccct taacgacggt caacgccgac acgcgggaaa tgggcgcgcg ctgcgccgaa
     1741 agcctgatcc gactcatccg cggcgaagac ccggccggcc tttccttcgc gggacggacg
     1801 cggctgatcg tgcgggaaag ctgcggcgcg gcgcggctgg aaaggaaggc gtcatgacgc
     1861 aaaaggtccg ctgggggctg atcggcgcga cgacgatcgc gcgtgaatgg atgatcgcgg
     1921 cgatccgcgc ggtcggcggc gaaatcgccg gggtgatgag ctccaacgcc gagcgcggcc
     1981 aaaaatacgc cgccgaattc ggcgttccgc tggcgacgac ctcgctcgac aagctgttcg
     2041 aggcctgcga cgcggtctat gtcgcgacga ccaacgatct ccattacgcg caagtcctgg
     2101 ccgcggcgaa ggccaagaag catgtcctgt gtgaaaagcc gctggcgctg accctccccg
     2161 aggcgcgcga gatggtcgcg gcctgcaagg cggccggcgt ggtcatgggc acgaatcatc
     2221 atttgcgcaa cgcggcgacc catcgcgcaa tgcgcgaggc gatcgcagcc ggcagaatcg
     2281 gccggccgct cttcgcccgc gtctgccacg ccgtctatct tcccgaacat ttgcagggct
     2341 ggcggatcaa agatcccaaa accggccccg gcgtcgtgat ggacatcacc gtgcacgatt
     2401 ccgacacgct gcgcttcgtg ctcggcgacg agccggaggc ggcgaccggc atggtccagc
     2461 gggccgggat gggcgcgggc gggatcgagg a
//
LOCUS       DATGJW010000381         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1316671, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000381 DATGJW010000000
VERSION     DATGJW010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1316671"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..942)
                     /gene="dnaX"
                     /locus_tag="VKU03_11515"
     CDS             complement(<1..942)
                     /gene="dnaX"
                     /locus_tag="VKU03_11515"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HLJ71937.1"
                     /translation="MNDLSPQDAGAPQPSLGLDVVPASSASGGAAAYRVLARKYRPIR
                     FEDLIGQEPMVRTIANSFATGRIHQAYILTGVRGVGKTTTARILARAFNYSVPGKIDA
                     PSVELAELGVHCQAIIESRHVDVIEMDAASHTGIDDIREIIENARYRPVSARTKVYII
                     DEVHMLSKAAFNGLLKTLEEPPEHVKFVFATTEIEKVPITVRSRCLRFDLKRIETGAL
                     TRHLEKICAAEGVAAEPEALALIARLAEGSVRDALSLLDQAIAHDGAAQVQAQSVRGM
                     LGLADRGAIIDLFDKTMRGDVAAAMATMRALHQAGADP"
     gene            complement(993..1089)
                     /gene="ffs"
                     /locus_tag="VKU03_11520"
     ncRNA           complement(993..1089)
                     /ncRNA_class="SRP_RNA"
                     /gene="ffs"
                     /locus_tag="VKU03_11520"
                     /product="signal recognition particle sRNA small type"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00169"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00169"
     gene            1207..2652
                     /locus_tag="VKU03_11525"
     CDS             1207..2652
                     /locus_tag="VKU03_11525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase"
                     /protein_id="HLJ71938.1"
                     /translation="MDIRMLIGGEAVGAAGGGVFERKNPFTGEIASRAPSGGVADAKK
                     AVEAAAAAFESWAAIGPNERRALLLKAADLLAGKAGEFGRIVAEETGATAPWGGFNVM
                     LASQMLREAASMTTQIAGEVIPSDKPGTTALAVRQPCGVVLGIAPWNAPVILGVRAIA
                     MPLACGNTVVLKASELCPATHSLIGQTMQEAGLPKGVVNVVTNAPADAAAVVEALIAH
                     PAVKRVNFTGSTRVGRIIAELAGRHLKPALLELGGKAPLVVLDDADLDAAVNAAAFGA
                     FAHQGQICMSTERIIVDEKIADAFVAKLAAKASKLPAGDPREPVVLGSMIDLAPAEKM
                     DALIADAVGKGAILVCGGKRNGTVVEATLLDKVTPAMRLYAEESFGPVKPVIRAKDTE
                     DAIRLANDTEYGLSAAVFGKDIMRAMAVAKRIRSGICHINGPTVQDEAQMPFGGVGAS
                     GYGRFGGKAAIAEFTDLRWITIEDPNQHYPF"
BASE COUNT          455 a          905 c          929 g          435 t
ORIGIN      
        1 gggatcggcg ccggcttggt gcaaagcccg catcgtcgcc atcgcggcgg cgacgtcgcc
       61 gcgcatcgtc ttgtcgaaca gatcgatgat ggcgccgcga tcggcgaggc cgagcatgcc
      121 tcgcacgctt tgcgcctgga cttgggcggc gccgtcatgg gcgatcgcct ggtcgagcaa
      181 cgacagcgcg tcgcgcaccg agccttcggc gaggcgggcg atcagagcga gcgcctccgg
      241 ctccgcggcg acgccctccg ccgcgcagat tttctcgaga tggcgggtca gcgcgccggt
      301 ctcgatccgc ttcaaatcga agcgcaggca gcgcgagcgc accgtgatcg gcactttctc
      361 gatttcggtc gtcgcgaaga cgaatttgac atgttccggc ggctcttcga gcgtcttcaa
      421 cagcccgttg aacgccgcct tggacagcat gtgcacttcg tcgatgatat agactttggt
      481 gcgcgccgag acggggcgat agcgggcgtt ctcgatgatc tcgcggatat cgtcgatgcc
      541 ggtatgggag gcggcgtcca tctcgatcac gtcgacatgg cgcgattcga tgatcgcctg
      601 gcaatggacg ccgagctcgg cgagctcgac gctcggcgcg tcgatcttgc cgggcacgga
      661 atagttgaag gcgcgggcga ggatgcgcgc ggtcgtcgtc ttgccgacgc cgcgcacgcc
      721 agtcaggata taggcctgat ggatgcggcc cgtcgcgaag gagttggcga tcgtgcgcac
      781 catcggctcc tggccgatca agtcctcgaa gcggatgggg cgatatttgc gcgccagcac
      841 ccgataggcg gccgcgccgc cggaagccga cgaggcgggg accacatcga ggccgagcga
      901 cggctgcggc gcgcccgcgt cctggggcga aagatcgttc atcgacgaaa acttcttgag
      961 ggcgatcgtt taagcggcgc gccggcgctt aaagcgggag gctggacaag aacccgccct
     1021 ggctcgttag ggctgcttcc ttccggacct gacccggttg gcgagtggtg cgtcccccgc
     1081 caacctcccg ccccctatgt ggactatggc cgcggcgggc gcaagggcga tgggggactc
     1141 tttcaattcg tttgccgcca aaccatttgg cgcttatttt cgcttgaaac aaaacgggag
     1201 cattccatgg acattcggat gttgatcggc ggcgaagcgg tcggcgcggc cggcggcggc
     1261 gttttcgagc gcaagaatcc gttcaccggc gagatcgcca gccgcgcccc gtcgggcggc
     1321 gtcgccgacg ccaaaaaggc cgtggaggcc gcggcggccg ctttcgagtc ctgggcggcg
     1381 atcgggccca acgagcggcg cgcgctgctg ctcaaggccg ccgacctgct cgccggcaag
     1441 gcgggcgaat tcggccggat cgtcgccgag gagaccggcg cgaccgcccc ttggggcggc
     1501 ttcaacgtca tgctcgcctc gcaaatgctg cgcgaagccg cctcgatgac gacgcaaatc
     1561 gccggcgagg tcattccctc cgacaagccg ggaacgaccg ccctcgccgt ccgtcagccc
     1621 tgcggcgtcg tgctcggcat cgcgccgtgg aacgcgccgg tcattctcgg cgtccgggcg
     1681 atcgccatgc cgctcgcctg cggcaatacg gtggtgctca aagcctccga actctgtccg
     1741 gccacccaca gcctgatcgg ccaaaccatg caggaggccg gcctgccgaa aggcgtcgtc
     1801 aacgtggtca ccaacgcgcc ggcggacgcc gcggcggtgg tcgaggcgct gatcgcccat
     1861 cccgcggtca agcgcgtcaa tttcaccggc tcgacccggg tcggccggat cattgcagag
     1921 ctcgccggcc gccacctgaa gccggcgctt ttggagctcg gcggcaaggc gccgctcgtc
     1981 gtgctcgacg acgccgatct cgacgcggcg gtcaacgccg ccgccttcgg cgccttcgcc
     2041 catcagggcc agatctgcat gtcgaccgag cggatcatcg tcgacgagaa aatcgccgac
     2101 gccttcgtcg cgaaactcgc cgccaaggcg tcgaaactgc cggccggcga tccgcgcgag
     2161 cctgtcgtgc tcggctcgat gatcgatctc gcgccggccg agaagatgga cgcgctgatc
     2221 gccgacgccg tcggcaaggg cgcgattctc gtttgcggcg ggaagcgcaa cggcacggtg
     2281 gtcgaggcga cgctgctcga caaggtgacg ccggcgatgc gcctctatgc cgaggaatcg
     2341 ttcggcccgg tcaagccggt catccgcgcc aaggatacgg aggacgcgat ccggctcgcc
     2401 aacgacaccg aatacggcct ctccgccgcg gtcttcggca aggacatcat gcgcgccatg
     2461 gcggtcgcca agcgcatccg ctccggcatc tgccatatca acgggccgac cgtgcaggac
     2521 gaagcgcaaa tgccgttcgg cggcgtcggc gccagcggct atggaaggtt cggcggcaaa
     2581 gcggcgatcg ccgagttcac ggatttgcgc tggatcacga tcgaggaccc gaaccagcac
     2641 tatccgttct gagaacctac cgacatttaa gaggagcgaa gcgacgaaac aatccattca
     2701 ccgggcgcgg cgccctggga gtgg
//
LOCUS       DATGJW010000382         8529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1004290, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000382 DATGJW010000000
VERSION     DATGJW010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8529
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1004290"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1119)
                     /locus_tag="VKU03_11530"
     CDS             complement(<1..1119)
                     /locus_tag="VKU03_11530"
                     /EC_number="2.1.1.37"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003886 - DNA
                     (cytosine-5-)-methyltransferase activity [Evidence IEA];
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA cytosine methyltransferase"
                     /protein_id="HLJ71939.1"
                     /translation="MPTFYEFFCGGGMVRAGLGARWDCRFANDLDAKKARAYAENWGA
                     DALRVGDIHALRPADLPGRADLAWASFPCQDLSLAGPGGGLAAKRSGAFWGFHALMSG
                     LAREGRSPTIIALENVVGALTSRAGADFIAICRALHGLGYRFGALTIDAAHFVPQSRP
                     RLFVVAVERSTQIPPALIGAGPSAAFASRALLRAQALLPAELAEDWLWWAPPAPPRCN
                     LALADIVETEAADPAWRSAEETAALIAILSPGSRAKLDAALSDGGRAVGAAYRRMRPD
                     GQGGRRMRAEIRFDGLAGCLRTPRGGSSRQFLLVIERGAPRARLVSAREAARLMGLPE
                     EYKLPLRYNEAYHLVGDGVAVPVVRFLAEHLFEPLAASG"
     gene            1431..2435
                     /locus_tag="VKU03_11535"
     CDS             1431..2435
                     /locus_tag="VKU03_11535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004340 - glucokinase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005536 - glucose binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0051156 - glucose 6-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucokinase"
                     /protein_id="HLJ71940.1"
                     /translation="MSQASFPYPVFACDTGGTNVRFSAVEKAGAPLGQVVHLKTGDYP
                     GLAEAVEAAAAKLGVKPRSVIACGAGPVDGRKLKLTNAPWLMDGPQVAAALGLAQGLL
                     LNDFEAQALSLPAIPEDWAMPIGPVRFGDPGPQVILGPGTGLGVGALVSAAGRFTPLA
                     SEACHTDFGPIDEEEMAIWPHLERVRGRVTTESVMAGPGLARLHRARLLAQGLPAPPI
                     DPAAVDARAHDDPASEEARSVALYWRLIARFAGDMGVTFVATGGVTLAGGVLPRMRDL
                     LDEKAFRAAFEAKAPVDALARRIPTRLVVQADSVLVGMAAIAAAPERYAIDYANRCWR
                     "
     gene            2543..3976
                     /locus_tag="VKU03_11540"
     CDS             2543..3976
                     /locus_tag="VKU03_11540"
                     /EC_number="1.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018045522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent succinate-semialdehyde
                     dehydrogenase"
                     /protein_id="HLJ71941.1"
                     /translation="MSYADVSLFIDGAWSKAGGAPIDILNPATGDRIGGVEHATKADL
                     DKALAAAARAFKTWSKVSAFERYKIMRKSAEILRGRADAIAKIMTLEQGKPLAQSKSE
                     TLNAADMIDWFAEEGRRAYGRLIPARVDGVRQMAVKSPVGPVAAFTPWNFPINQAVRK
                     ISGALATGCTIIIKGPEETPASCAELVRAFADGGVPAGVINLVFGVPAEISEYLIPHP
                     AIRKVSFTGSTAVGKQLAALAGLHMKRITMELGGHAPALVFDDADVDKAVKQLATGKY
                     RNAGQICVAPTRMLVQRKVYEPFVEKFVSEAKKIKVGDGLDPDTVMGPLAHLRRVEAM
                     EAFVADAVSHGAELRTGGKRIGNKGFFFEPTVVTRAPKTARIMNEEPFGPLAIINPFD
                     DYDEAVAEANRLDYGLAAYAYTKSAETSAKLGEAVESGMITINHMGLALPEVPFGGVK
                     DSGFGSEGGAEALEAFFNTRFVTQLPA"
     gene            4062..4511
                     /locus_tag="VKU03_11545"
     CDS             4062..4511
                     /locus_tag="VKU03_11545"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71942.1"
                     /translation="MTRNLIVGRSALLALGSAAMFLALSAAARADCPDDLKKLTERRV
                     SLLNEINAMAAASKKAKKPMEPGIVCAKARGLTSAENALLAYMDKNKDWCGIPDEVLQ
                     SLKTSHAKTLDFGNRACVAAAKFKKMQEQQAAGGAPGGPPPLPAGPL"
     gene            4517..5458
                     /gene="ubiA"
                     /locus_tag="VKU03_11550"
     CDS             4517..5458
                     /gene="ubiA"
                     /locus_tag="VKU03_11550"
                     /EC_number="2.5.1.39"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591889.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008412 - 4-hydroxybenzoate
                     octaprenyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxybenzoate octaprenyltransferase"
                     /protein_id="HLJ71943.1"
                     /translation="MTRDANLLPDARPASLVLRLTPAWALPYVQLARLDRPIGWQLLL
                     APCWQSAALAGLYRNAGPDLLHLVLFLIGAIAMRGAGSTYNDILDRKLDAGVERTKGR
                     PLPSGRIGVGAAVVFLVAQSLVGLAVLRCFNGFSIVLGIASLAVVAVYPLMKRVTQWP
                     QAVLGLAFSWGALMGWAADYHSLAAPALLLYASAFCWTIGYDTIYALQDARDDAIVGI
                     KSTARLFGANVRLGVGLFYLATALLAQAALWTVGASVIAELGLLLYCLHFVWQLRRLR
                     EPNPAMALRLFRANWHAGLILFFGLAIEGWVRHALIG"
     gene            complement(5740..6492)
                     /locus_tag="VKU03_11555"
     CDS             complement(5740..6492)
                     /locus_tag="VKU03_11555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71944.1"
                     /translation="MFEQAEWLLAAHRHRRAFAKAQLLPLSEKVFPSNGAKGHALAER
                     YFADVLRYADATSLQLRLEPSADSGRYKWDGSRYAVQQKSGAAGRYWRREGAIAYDST
                     LLEIPADLIAVLAHEVSHAILDLGAARPPPGDPAFEELRTDFTAVFLGFGFYLAQFRA
                     DTRISSPEAAREFKKLYMYYMNLRELCFATALFCAVREVERKPVFRRAPAAAAPLLKR
                     AFADLDSNADRVALLRAAAHRVAALSPIMPPA"
     gene            complement(6727..>8529)
                     /locus_tag="VKU03_11560"
     CDS             complement(6727..>8529)
                     /locus_tag="VKU03_11560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018324475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HLJ71945.1"
                     /translation="RRLGCQAKICGDLVVDVPPDSQVHRQVVRKRAESHPIEVDPVVR
                     LYYVEVREPDMHDPSSDLRRLQSALKSQWSLGETVADLAVLRGLQKTLRAGEWKATVA
                     LRNGRDIVAVYPGFRDRALGVAFDIGSTTIAAHLCDLSSGDVLASAGAMNPQIRFGED
                     LMSRVSYVMMNPGGDKELTESVRGALDALIGDVVNEAAAERQDVVEVTLVGNPIMHHL
                     ALGLDPTELGGAPFALTVDGPQEARAAELGLSVAPGAYVYCLPCIAGHVGADTAGMVL
                     AEGPYLRDDLTLLVDVGTNAEIVFGNRGRLLACSSPTGPAFEGAQISCGQRAAPGAVE
                     RVRIDRTTLEPRFKVIGCELWSNEEGFAEATASTGITGICGSGVIEVIAEMYLAGIVS
                     EDGVIDGALAEKNKRIEPVGRTFGYLLHDGEPRLMIYQTDVRAIQLAKAALYAGAKLL
                     IERFGAAAPERITLAGAFGSHIDPTYAMTLGLIPDCDLAKVAAAGNAAGTGARIALLN
                     AAARREIEDVVRRIEKVETAIEPRFQEHFVAAMAIPNKVDAFPNLGKAVRLPERGAGG
                     AERGPRRRRREAGAGATEAEPLAKIPPPPPRRTY"
BASE COUNT         1426 a         2882 c         2864 g         1357 t
ORIGIN      
        1 gccgcttgcg gccagcggct cgaacaaatg ctcggcgaga aagcgcacca ccggaaccgc
       61 cacgccgtcg ccgacaagat ggtacgcctc gttgtagcgt aggggcagct tgtactcttc
      121 cgggaggccc atcagccgcg ccgcctcgcg cgcggagacg agccgcgcgc gcggcgcgcc
      181 gcgctcgatg acgagcagga actgccggct cgaaccgccg cgcggcgtcc gcaggcagcc
      241 ggcgagcccg tcgaatcgga tttccgcgcg catgcgccgg ccgccttgcc cgtccggacg
      301 catgcgccga tacgccgctc cgacggcgcg tccgccgtcg ctcaatgcgg cgtcgagctt
      361 ggcgcgtgat ccgggcgaga ggatcgcgat cagcgccgcc gtctcctcgg cgctgcgcca
      421 ggccggatcg gccgcttccg tctcgacaat atcggcaagc gccaggttgc agcgcggcgg
      481 cgcgggcggc gcccaccaca gccaatcctc ggcaagttca gccggcagga gcgcctgcgc
      541 ccgcaaaagg gcgcgcgagg cgaaagcggc gctgggaccc gccccgatca gcgccggcgg
      601 aatttgcgtc gagcgctcga ccgcgacgac gaacagccgg ggacgcgatt gcggcacgaa
      661 atgcgcggcg tcgatcgtca gcgcgccgaa gcggtagccg aggccgtgca gagcgcggca
      721 gatggcgatg aaatcggctc cggctctgga ggtcaacgcg ccgacgacat tttccagcgc
      781 aatgattgtc gggcttcgcc cctccctcgc cagcccgctc atcagcgcgt ggaagcccca
      841 gaaagcgccg gagcgcttgg cggccaggcc gccgccgggg ccggccaagg acaggtcctg
      901 gcagggaaac gaagcccagg cgagatcggc gcggccgggc agatcggcgg gacggagcgc
      961 atggatgtcg ccgacgcgaa gcgcatcggc gccccaattc tccgcatagg cgcgcgcctt
     1021 cttggcgtcg aggtcgttgg cgaagcggca gtcccagcgc gcgccgagcc cggcccgaac
     1081 catgccgccg ccgcagaaga attcgtagaa agtcggcata cggcgaatcg ctccgcgcca
     1141 aggctagctt gcgccctcgc ggcggtcacg gcccgaccgg tcaaattcga cgggatcagc
     1201 cccgctcccc ggcgtctttc tcgaagctcg taatttcccg caaccgaggc gcggcgacat
     1261 ttgtccgcgc tgcgccagga cgccgacccg tagccgcctt aagttcgcgc cgttcgggat
     1321 cgtaacccaa ttgcggcggg ttgtgcgtcg gcgcggcgat gggggattgc cgcgacgcaa
     1381 agagcgccgg cgccttgacg ggcgcggcgc cgccaaggtc aatgagcgcg atgagccaag
     1441 cgtcctttcc ctatccggtc ttcgcctgcg acaccggcgg caccaatgtt cgcttttcgg
     1501 ccgttgaaaa ggccggggcg ccgctgggcc aggtcgtcca tctcaagacc ggcgactatc
     1561 ctggcctcgc cgaggcggtg gaggccgccg cggccaagct cggcgtcaag ccgcgctcgg
     1621 tgatcgcctg cggcgccggg ccggtggacg gacgcaagct gaagctgacc aatgcgccct
     1681 ggctgatgga cgggccgcaa gtcgcggcgg cgctcggcct ggcgcaaggg ctgctgctca
     1741 acgatttcga ggctcaggct ctttccctgc cggccatccc cgaagactgg gcgatgccga
     1801 tcggtccggt ccggttcggc gatcccggcc cgcaagtcat tctcgggccc ggcaccggcc
     1861 tcggcgtcgg cgcgctggtt agcgcggccg ggcggttcac gccgctggcc tcggaagcct
     1921 gccacaccga tttcgggccc atcgacgaag aggagatggc gatctggccg catctcgaac
     1981 gggtgcgcgg ccgggtgacg acggaaagcg tgatggccgg gccgggcctg gcgcggctgc
     2041 atcgggcgcg gctgttggcg cagggcctgc cggcgccgcc gatcgacccg gccgccgtcg
     2101 acgcgcgggc gcatgacgac ccggcgagcg aggaggccag gagcgtcgcg ctctattggc
     2161 ggctgatcgc ccgcttcgcc ggcgatatgg gcgtgacctt tgtcgccacc ggcggcgtga
     2221 ccttggccgg cggcgtgctg ccgcgcatgc gcgacctgct cgacgagaag gctttccgcg
     2281 ccgccttcga ggccaaggcg ccggtcgacg ccctggcgcg gcgcatcccg acgcggctcg
     2341 tggttcaggc cgattccgtg ctcgtcggca tggccgcgat cgccgccgcg ccggagcgct
     2401 atgcgatcga ctacgccaat cgctgctggc ggtagccgcc aggcgcgacg cagccgcgcc
     2461 gccggtcttc cttgccgccg cggcggaaag cgctaaatag agaccattcc cccgcaacgc
     2521 ctcttaagga aaggccttcc ccatgtccta tgccgacgtt tcgctgttca tcgacggagc
     2581 atggagcaag gcgggcggag cgccgatcga cattctcaat ccggcgaccg gcgaccgcat
     2641 cggcggcgtc gagcatgcga cgaaagccga tcttgacaag gccttggcgg cggcggcgcg
     2701 cgccttcaag acctggagca aagtctcggc tttcgagcgc tacaagatca tgcgcaaatc
     2761 ggcggaaatc ctgcgcggcc gcgccgatgc gatcgccaag atcatgacgc tcgagcaggg
     2821 caagccgctc gcgcaaagca aatccgagac gctcaacgcc gccgacatga tcgactggtt
     2881 cgccgaggaa ggccggcgcg cctatggccg gctgattccg gcgcgggtcg acggcgtgcg
     2941 ccagatggcg gtgaaaagcc cggtcgggcc ggtcgccgcc ttcacgccct ggaactttcc
     3001 gatcaaccag gcggtgcgca aaatctccgg cgcgctggcc accggctgca ccatcatcat
     3061 caaagggccg gaagagacgc cggcttcctg cgccgagctc gtgcgcgctt tcgccgatgg
     3121 cggcgtgccg gccggcgtca tcaatctcgt cttcggcgtg ccggccgaaa tctcggaata
     3181 tctcatcccc catccggcga tccgcaaggt gagcttcacc ggctcgaccg ccgtcggcaa
     3241 gcagctcgcc gctctcgccg gcctgcatat gaagcgcatc accatggagc ttggcggcca
     3301 tgcgccggcg ctcgtcttcg acgacgccga tgtggataag gcggtcaagc agctcgcgac
     3361 cggcaaatac cgcaatgccg gccagatctg cgtcgcgccg actcgcatgc tggtgcagcg
     3421 caaagtctat gagccgttcg tcgagaaatt cgtcagcgag gccaagaaga tcaaggtcgg
     3481 cgacgggctc gaccccgaca ccgtgatggg cccgctggcg catctgcggc gcgtcgaggc
     3541 gatggaggct ttcgtcgccg acgcggtcag ccatggcgcg gaattgcgca cgggcggcaa
     3601 gcgcatcggc aataagggct tcttcttcga gccgaccgtc gtcacccgcg cgccgaagac
     3661 ggcgcggatc atgaacgagg agccgttcgg cccgctcgcc atcatcaatc cgttcgacga
     3721 ttacgacgag gcggtcgccg aggcgaaccg gctcgattac ggcctcgccg cctacgccta
     3781 tacgaaatcg gccgagacca gcgccaagct cggcgaggcg gtcgagagcg gcatgatcac
     3841 gatcaaccat atgggccttg ctctcccgga agtgccgttc ggcggcgtca aggattcggg
     3901 cttcggcagc gaaggcggcg ccgaggcgct ggaggccttc ttcaacacaa gattcgtcac
     3961 ccagctcccc gcgtgaggcc gagggataag cggcgcttcc ggtttcctcg cggcgcgaaa
     4021 tggtctatca gggccgcgag caaaacgatg gaggcgccgc tatgacgcgc aatttgatcg
     4081 tcggccgctc ggccctgctc gcgctcggct cggccgctat gttcctggcg ttgagcgcgg
     4141 cggcgcgcgc cgactgcccc gacgacctca agaagctgac cgagcgccgc gtatccctgc
     4201 tcaatgaaat caacgccatg gccgccgcga gcaaaaaggc gaaaaagccg atggagccgg
     4261 ggatcgtctg cgccaaggcg cgcggcctga ccagcgccga gaacgcgctg ctcgcctata
     4321 tggacaagaa caaggactgg tgcggcattc ccgacgaggt cctgcaaagc ctgaagacga
     4381 gccacgccaa gacgctcgat ttcggcaatc gggcctgcgt cgccgcggcc aagttcaaga
     4441 agatgcagga gcagcaggcg gccggcggcg cgcccggcgg accgccgccc ttgccggccg
     4501 ggccgctttg aacgcgctga cgcgcgacgc caatctcctt cccgacgcca ggccggccag
     4561 cctcgtcctt cgcctgacgc cggcctgggc gctgccctat gtccagctcg cccgactcga
     4621 ccggccgatc ggctggcaat tgctgctcgc gccatgctgg caatcggcgg cgctcgccgg
     4681 cctctaccgc aatgccggac cggaccttct ccatctcgtt cttttcctga tcggcgcgat
     4741 cgccatgcgc ggcgccggct cgacctataa cgacattctc gaccgcaagc ttgacgccgg
     4801 cgtcgagcgc accaagggcc gacccctgcc ctcgggccgg atcggcgtcg gcgctgctgt
     4861 cgttttcctg gttgcgcaat cgcttgtcgg cctcgccgtg ctgcgctgct tcaacggctt
     4921 ttcgatcgtg ctcgggatcg cttcgctcgc cgtggtcgcg gtctacccgc tgatgaagcg
     4981 cgtcacgcaa tggccgcagg ccgtgctcgg cctcgctttt tcctggggcg cgctgatggg
     5041 ttgggcggcg gactatcatt cgctcgcagc gccggccctg ctcctctacg cctccgcgtt
     5101 ctgctggacg atcggctacg acacgatcta cgcgctgcag gacgcgcgcg acgatgcgat
     5161 cgtcggcatc aaatcgacgg cgcggctctt cggcgccaat gtccggctcg gcgtcggcct
     5221 gttctatctg gcgacggctt tgctggcgca ggcggcttta tggaccgtcg gcgcaagcgt
     5281 gatcgcagaa ctcggcctgc tgctctactg cctgcatttc gtctggcaat tgcggcgcct
     5341 gagagagccg aatccggcta tggccctgcg gctgttccgc gccaactggc atgcggggct
     5401 gatcctgttc tttggcctgg cgatcgaggg ctgggtacga cacgcgctga tcgggtgacc
     5461 gcgctcctga agggagcctt cgggaaaaag ggctcgcgcc aaattttacg cccgtctgcc
     5521 caaatatttc caaatatttc caaatttttt cttggccgaa tcgggccttt tcaaagggtt
     5581 aaagcgcgga aaaattgcaa acgcattttc cgcgtttttt cacgctctcg accgagccgc
     5641 tcatcatacc gtcgaaggct ctattcggct agaaattgtt cgaggaaatt gtttaggcgc
     5701 cggcgtggga gccacccggg ttgcggtgtt cgcgcttcgc tacgccggcg gcatgatcgg
     5761 cgaaagcgcc gcgacgcgat gggccgcggc gcgcaggagg gcgacgcgat cggcgttgct
     5821 gtcaaggtcg gcgaaggcgc gcttcaacag cggcgcggcg gcggctggcg ccctgcgaaa
     5881 aaccggcttg cgctcgacct cgcgcaccgc gcaaaacaaa gccgtcgcga agcagagttc
     5941 gcggaggttc atatagtaca tgtagagctt cttgaattcc cgcgcggctt ccggcgaaga
     6001 aattcgggta tcggcgcgga actgggcaag atagaaaccg aagccgagga agacggcggt
     6061 gaagtcggtg cgcagctcct cgaaagccgg atcgccgggc ggcggccgag ccgcgccgag
     6121 gtccaaaatg gcgtgagaga cctcatgcgc gaggaccgcg atcaggtcgg cgggaatttc
     6181 gagcagcgtc gaatcgtaag caatcgcgcc ctcgcgccgc cagtacctcc cggcggcgcc
     6241 cgacttttgc tggacggcgt agcgcgagcc gtcccacttg taccggccgc tatcggcgct
     6301 cggctccaag cggagctgca aagaggtcgc atcggcgtag cgcagcacgt cagcgaaata
     6361 ccgctcggca agcgcatggc ctttcgcgcc atttgacgga aagaccttct cggatagcgg
     6421 caagagctgc gccttggcga aagcgcggcg atgacgatgc gcggcgagca accactccgc
     6481 ctgctcgaac atccactccg tcatttcagg ctcaagcaag ggcttgctgc ggaacggcca
     6541 cacgaatgtc tccggcgcgg aggaagcggc ggagcttcgt aagacatttc caagcgcaat
     6601 gcaaccttaa gttgcaaacc cagcggcttc cgcgtcagcg ggaaatccag actggccagt
     6661 tcacatacac tgctttctgg atttccgctt ttcgcgggaa tgacggcgcc caatgcaggg
     6721 ttcgtttcag tacgtccgcc gcggcggagg cggggggatt ttcgccagcg gttcagcctc
     6781 ggttgcgccg gcgcccgcct cgcgacgcct gcgcctcggc ccccgctccg cgccgcccgc
     6841 cccgcgctcc ggcaagcgca ccgccttgcc aaggttcggg aacgcgtcga ccttgttcgg
     6901 gatcgccatg gcggcgacga aatgctcctg gaagcgcggc tcgatcgccg tctcgacctt
     6961 ttcgattcgg cgcaccacgt cttcgatctc gcgccgcgcg gcggcgttga gcagcgcgat
     7021 gcgcgcgccc gtccccgccg cgttgccggc ggcggcgact ttggcgagat cgcaatcggg
     7081 gatgaggccg agcgtcatcg cataggtcgg atcgatatgg ctgccgaacg cgccggccag
     7141 cgtgatgcgc tcgggcgcgg cggcgccgaa gcgctcgatc agcagcttgg cgccggcata
     7201 gagcgccgcc ttggcgagtt gaatggcgcg aacatcggtc tgatagatca tcaggcgcgg
     7261 ctcgccgtca tgcagcagat agccgaaggt gcggccgacc ggctcgatgc gcttattctt
     7321 ttcggcgagg gcgccgtcga tcacgccgtc ttccgaaacg atcccggcga gatacatctc
     7381 ggcgatgact tcgataacgc ccgagccgca gatgccggta atgccggtcg aagccgtcgc
     7441 ttcggcgaag ccttcttcgt tcgaccagag ctcgcaaccg atcaccttga aacgcggctc
     7501 caaagtcgtg cgatcaatgc gtacccgctc gaccgcgccc ggggcggcgc gttggccgca
     7561 ggaaatctgc gcgccttcga aggcgggccc ggtcggcgag gagcaggcga gcaagcggcc
     7621 gcgattgccg aagacgatct cggcgttcgt gccgacatcg accagcagcg tgaggtcgtc
     7681 gcgaagatac gggccttcgg ccaggaccat gccggccgta tcggcgccaa catggccggc
     7741 aatgcaagga aggcaataga cataggcgcc tggcgcgacg ctcaggccca gttccgccgc
     7801 gcgcgcctcc tgtgggccgt cgacggtcag ggcgaaaggc gcgccgccaa gctcggtcgg
     7861 atcgaggccg agagcgaggt gatgcatgat cggattgccg accagcgtga cctctacgac
     7921 gtcctggcgc tcggccgcgg cctcgttgac gacatcgccg atcagcgcgt cgagtgcgcc
     7981 gcgcacgctc tcggtcaatt ccttgtcgcc gcccggattc atcatcacgt acgaaacccg
     8041 gctcatcagg tcttcgccga accggatttg cggattcatc gccccggccg aggcgagcac
     8101 gtcgccgctc gaaagatcgc agagatgggc ggcgatggtg gtcgagccga tgtcgaacgc
     8161 gacgccgagc gcgcggtcgc ggaagccggg atagacggcg acgatgtcgc ggccgttgcg
     8221 cagcgcgacg gtcgccttcc actcgccggc gcgcaaagtc ttctgcaagc cgcgcaagac
     8281 ggcaagatcg gcgaccgtct cgccaagcga ccattggctc ttcaaagccg attgcagccg
     8341 gcgcaaatcg ctcgacggat catgcatatc gggctcgcgc acctcgacat agtagaggcg
     8401 cacgaccgga tcgacctcga tcggatgcga ttcggcgcgc ttgcgcacga cctggcgatg
     8461 gacctgcgaa tccggcggca cgtcgacgac gagatcgccg cagattttcg cctggcagcc
     8521 gaggcggcg
//
LOCUS       DATGJW010000383         2894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1110662, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000383 DATGJW010000000
VERSION     DATGJW010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2894
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1110662"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1239)
                     /gene="lysA"
                     /locus_tag="VKU03_11565"
     CDS             complement(<1..1239)
                     /gene="lysA"
                     /locus_tag="VKU03_11565"
                     /EC_number="4.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911728.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008836 - diaminopimelate decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diaminopimelate decarboxylase"
                     /protein_id="HLJ71946.1"
                     /translation="MHHFAYRNGVMHAEDVDLRDIAAATGTPFYCYSTATIERHYRVF
                     SDAFAGAKALVCYAMKANSNQAVLRLLARLGAGMDVVSEGELRRARAAGVPGSKIIFS
                     GVGKTREEFALGLDEDILCFNVESEPELAALSEVAASRGQKARIAVRVNPDIDAGAHA
                     KISTGKSENKFGVPISRAREVYARARTLPGIAVTGVDMHIGSQIVDLQPFDDAFALLA
                     DFVRQLRADGHAIDHIDLGGGLGVPYRRDNDPPPHPERYAEIVQRRTAGLDCWLALEP
                     GRLIVGNAGVLVTKVLYVKRGAGRNFVIVDAAMNDLIRPTLYEAYHEILPVIEPDPAR
                     PRLLADFVGPVCESGDYLGLGRELAQPEAGDLFAVMTAGAYGAVQAGTYNSRLLAPEI
                     LVHGAAFATIRPRPSYQDLIG"
     gene            complement(1249..1473)
                     /locus_tag="VKU03_11570"
     CDS             complement(1249..1473)
                     /locus_tag="VKU03_11570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007607415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipoprotein"
                     /protein_id="HLJ71947.1"
                     /translation="MNRAFKVPLAALALTAAAGLGGCGRAGPLEPPLDAQAAAKQASN
                     DENNSGLPQPHPRPKPIAAPKQPFLLDPLL"
     gene            1725..>2894
                     /locus_tag="VKU03_11575"
     CDS             1725..>2894
                     /locus_tag="VKU03_11575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011511248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding protein"
                     /protein_id="HLJ71948.1"
                     /translation="MFERDNGFTRSVSRRSLLQAGAGVAGGLMLPTGFVAGARAEDQP
                     AIGTWPAGSSGSSVTIGVAVPRTGTYAQSGEDELKGIELAVEHINSGDPLIKAISPKT
                     TKGVLGKQVKFVVADSEAKPNTAVQEQSRFITEDKVVMMIGSTSSAVAVAMNKLAQRE
                     KVLYLAAISGSNDTTGKDCVRYGFRQCFYGQTAAAAIGPVLVKNFGKNRKAAFMTPDY
                     TYGHTVTKSVSDYLTANGGWTNVTDQVSPLGTTDFSQYLTNIANSGAEFLINVNWGRD
                     AVLSTQQAHQFGLIPKMTLVIPYQTPFLAKEVGPEIMGGVFAATDFWWTLEDKYPLAK
                     MFVEAFDKKYGYKPEWGANNSYMQLAMWADSVENAGTFYPPDVIKSYESGRTMQST"
BASE COUNT          545 a          914 c          968 g          467 t
ORIGIN      
        1 gccgatcaag tcctggtaac tcggacgcgg acgaatggtt gcgaaggccg cgccatggac
       61 cagaatctcc ggcgcgagca ggcgcgaatt ataggtcccc gcctggaccg cgccataggc
      121 gccggcggtc atcaccgcga agagatcgcc ggcttcaggc tgggccaatt cgcgcccgag
      181 gccaagataa tcgccgcttt cgcagaccgg gccgacgaaa tcggcgagaa ggcgcgggcg
      241 ggccgggtcg ggttcgatta ccggaagaat ttcgtgataa gcctcgtaaa gggtcggtcg
      301 gatcagatcg ttcatcgccg cgtcgacaat gacgaagttc cggcccgcgc cgcgcttgac
      361 gtaaaggact ttcgtcacca gcacgccggc attgccgacg atcaggcggc ccggctccag
      421 cgccagccag caatcgagcc cggcggtgcg gcgctggacg atttcggcat agcgctcggg
      481 atgcggcggc gggtcgttgt cgcggcgata gggaacgcct aagccgccgc cgagatcgat
      541 gtggtcgatg gcgtggccgt cggcgcgcaa ttggcggacg aaatcggcga gcagggcgaa
      601 ggcgtcgtcg aacggctgaa gatcgacgat ctgcgagccg atatgcatgt cgacgccggt
      661 cacggcgatt ccgggcagcg tcctggcgcg ggcgtaaacc tcccgcgccc gcgaaatcgg
      721 cacgccgaac ttgttctcgg atttgccggt ggaaatcttg gcgtgggcgc ccgcgtcgat
      781 gtccggattg acgcggacgg cgatgcgcgc cttttgcccg cgcgaggcgg cgacttcgga
      841 caaggccgcc aattccggct cggattcgac gttgaagcag aggatgtcct catcgaggcc
      901 gagcgcgaat tcctccctcg tcttgccgac gccggaaaag atgattttgg agccgggcac
      961 gcccgccgcc cgggcgcggc gcaattcgcc ttccgagacc acgtccatgc cggcgccgag
     1021 ccgcgccaga aggcgcagca cggcctggtt ggaattggcc ttcatggcgt agcagacgag
     1081 cgccttggcg cccgcgaagg cgtccgaaaa gacccggtaa tggcgttcga tcgtcgccgt
     1141 cgaatagcag tagaaaggcg ttccggtcgc ggcggcgatg tcgcgcaagt cgacatcctc
     1201 ggcgtgcatg acgccgttgc gataggcgaa atgatgcatc ggcgttcttt aaaggagcgg
     1261 atcgaggagg aacggctgtt tgggcgccgc gatcggtttg ggccgcggat gaggctgcgg
     1321 caggcccgaa ttgttctcgt cgttgctcgc ctgcttggcc gcggcttgcg cgtccagcgg
     1381 cggctcgagc ggtccggcgc ggccacagcc gccgagcccc gcggcggcgg tcagcgcaag
     1441 ggcggcgagc gggaccttga aagcgcgatt cacgaaaagc tcctaaagac gcgctcatta
     1501 tacaagaacg cgccggcaac gcgagccggc ggcgcgccgg ccgttgacgc gcgccttgtt
     1561 cctgttcaga cttttgtcca attccaatcg gcggcgcccg ccattaccct gaccgagctt
     1621 gtaaggatca atgcgcgccg ccggatcgga ccggccgggc gcggcgatgg cagaccgatc
     1681 cgccggtcgc ccgccaaaga atcatctcaa agggaggagt tccgatgttt gaaagggaca
     1741 atggatttac gcgcagcgtg tcgcggcgca gcctgcttca agccggcgct ggcgttgcag
     1801 gcggcctcat gctgccgacc ggcttcgtcg ccggcgcgcg cgccgaggac cagccggcaa
     1861 tcggcacttg gccggccggc agctccggct cgtcggtcac gatcggcgtc gccgtgccgc
     1921 gcaccggcac ctacgcccag tcgggcgagg acgagctcaa gggcatcgag ctcgcggtcg
     1981 agcacatcaa tagcggcgat ccgctgatca aggcgatctc gcccaagacg acgaaaggcg
     2041 ttcttggcaa gcaggtcaaa ttcgtcgtcg ccgattcgga ggccaagccg aacaccgcgg
     2101 tgcaggagca gtcgcgcttc atcaccgagg acaaggtcgt catgatgatc ggctcgacat
     2161 cgagcgcggt cgccgtggcg atgaacaagc tggcgcagcg cgaaaaagtg ctgtacctcg
     2221 cggcgatttc cggctcgaac gacacgaccg gcaaggattg cgtgcgctac gggttccgcc
     2281 agtgcttcta cggccagacc gccgccgccg cgatcggccc ggtcctggtc aagaatttcg
     2341 gcaagaaccg caaagccgcg ttcatgacgc cggattatac gtacggccac accgtcacca
     2401 agtcggtgag cgactatctg acagcgaatg gcggctggac gaacgtgacc gatcaggtct
     2461 cgccgctcgg cacgacggat ttcagccagt acctgaccaa catcgccaat tccggcgccg
     2521 agttcctgat caacgtgaac tggggccgcg acgcggtgct gtcgacccag caggcgcacc
     2581 aattcggcct catcccgaaa atgacgctgg tcatccccta ccagacgccg ttcctcgcca
     2641 aagaggtcgg gccggaaatc atgggcggcg tcttcgccgc gaccgatttc tggtggacgc
     2701 tcgaggacaa atatccgctg gccaagatgt tcgtcgaggc gttcgacaag aaatacggct
     2761 acaagccgga atggggcgcc aacaattcct acatgcagct cgccatgtgg gcggactcgg
     2821 tcgagaacgc cggcaccttc tatccgccgg acgtgatcaa gtcctatgaa agcggccgga
     2881 ccatgcagtc gacc
//
LOCUS       DATGJW010000384         8509 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1317356, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000384 DATGJW010000000
VERSION     DATGJW010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8509)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8509)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8509
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1317356"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..864
                     /locus_tag="VKU03_11580"
     CDS             <1..864
                     /locus_tag="VKU03_11580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006724924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HLJ71949.1"
                     /translation="GDMMFPTYRQPGLLIAREWSLYDMACQVFANAGDRMKGRQLPVL
                     YSVPQEGFFTISGNLATQFIQAVGWAMASAYKGDTRIAAGYIGDGATAEGDFHYALTF
                     AAVYRAPVILNVVNNQWAISTFQGFAGGENATFADRAIGYGLPGLRVDGNDLLAVIAA
                     TRWAAERARANFGATLIELVTYRAAAHSTSDDPSRYRPADEWEHWPLGDPIPRLKQHL
                     IARGEWSEARHTQLGAEVEDEVQSAAKKAEALGVLGKGAQHPSQTMFEDVYREMPPHL
                     KRQRQEFEAGR"
     gene            861..1880
                     /locus_tag="VKU03_11585"
     CDS             861..1880
                     /locus_tag="VKU03_11585"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130261.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HLJ71950.1"
                     /translation="MSAMTLSQALNSAMDVMLGQDDNVLLMGEDIGYFGGVFRCTEGL
                     QKKYGPRRVLDTPLSEGGIVGAAIGMGVYGLRPVVEIQFADYSYPAFDQLASEAARLR
                     YRSAGEFWAPLTVRTPYGGGIYGGQTHSQSPEALFTHVCGLKTVVPSNPYDAKGLLIA
                     AIEEDDPIIFLEPKRLYAGPFDGHHDRPATSWEGHPHSDVPDGHYRVPLGKAKIARPG
                     NSVTVLAYGTMVHVAIAAAQDCGVDAEVIDLRTLIPLDLDTIVASVKKTGRCVVVHEA
                     TLTCGYGAELVALVQQHCFFWLETPIERVAGWDTPYPYAFEWDYFPGPKRVGAALKKA
                     VDGKA"
     gene            1877..3103
                     /locus_tag="VKU03_11590"
     CDS             1877..3103
                     /locus_tag="VKU03_11590"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015314820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HLJ71951.1"
                     /translation="MKYVFKLPDLGEGTTKAEIVKWHVATGDIVREDQPLVDMMTDKA
                     TMEIPAPVNGAILELHGEPGDVRAVGAELVVFDVASAPHERAAEAAPPPRQAEPAAAA
                     RPLEKERPVEKRQAKPIASPVVRRRAAELGVSLSDVPASGPDGRVLREDLEAFAAGRR
                     RPAAARSEDRREGDIEEIKVIGLRRAIAERMQDAKRRIPHITYVEEIDISALEDLREH
                     LNRDLADRRVKLTILPFLIRAMVRGVARYPQCNALFDDEAGIVRRHRAVHVGIATQTA
                     SGLMVPVLRHAEALDLFESAEEIARLAERARDGKAARDELVGSTITISSLGPLGGIAS
                     TPVINPPEVAIVGVNKIVERPVIQRGQIAVRKMMNLSSSFDHRIIDGWDAASFIQAIK
                     GYLEQPATLFMNEHSD"
     gene            3244..4287
                     /locus_tag="VKU03_11595"
     CDS             3244..4287
                     /locus_tag="VKU03_11595"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 9 protein"
                     /protein_id="HLJ71952.1"
                     /translation="MAQPLSGIGDMIWHVPHIRALARHVGAPVTLIASFRSAADQIFK
                     ADDAVADVLFIERRPKGRTGRHDGPLGMIRLVSALRARQFDALVLLHHGRTLAFAALC
                     AGVRQRSGYGVGLQRAFLNRGPYLAADRLRLHPFERASAWLAAGGVELAESEPRLAIA
                     PDSLAKARLFLGEADPKPVAIGIGSSEPFKQWGAARFAELAEKLIEAGWTRLVLIGGK
                     PERPLAEDICARLGAKAGGVLVSTGWDIADVAALLSLSAFYVGNDTGFMNMAAAAGLR
                     SFCLFGGSAPFRHSRNIMPILPPDGKPDKNGGMARITAAAAMAAIAAQGLAPPREEAR
                     YGAGAAGLPGRSL"
     gene            complement(4245..4796)
                     /locus_tag="VKU03_11600"
     CDS             complement(4245..4796)
                     /locus_tag="VKU03_11600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ71953.1"
                     /translation="MLSQTSIRELDAAETEARLTELADILVDAVAHGASVNFLAGFAR
                     DDGRAFWRRQIDDIAGGGKRLFVGERGGRLVGTVLLIYAPQPNAPHRAEIGKMLVLSS
                     ERRQGLGRRLLGAAEEAALQAGRTLLLLDTESGSSGDRLYRSAGWREYGRVPDHAYRV
                     DGRLAETTMFYKLLPGRPAAPAP"
     gene            complement(4818..5387)
                     /locus_tag="VKU03_11605"
     CDS             complement(4818..5387)
                     /locus_tag="VKU03_11605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018859751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="XRE family transcriptional regulator"
                     /protein_id="HLJ71954.1"
                     /translation="MSLAESDIDARLAARIRALRAERGLTLEGLASLAEVSRAMLSRI
                     ERGESSPTAQLLNKICGGLRITLSELFAESGKPASPLCRRADQPAWRDPASGYVRRNV
                     SPAATGSAIDLVEIEFPPGASVAFANDRIAGKDQHIWLLDGAIEIDLDGEIVRLEAGD
                     CFYMRFGKPVRFRNPSSRPARYAVVLSRS"
     gene            complement(5810..7093)
                     /locus_tag="VKU03_11610"
     CDS             complement(5810..7093)
                     /locus_tag="VKU03_11610"
                     /EC_number="2.5.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-acetylhomoserine
                     aminocarboxypropyltransferase"
                     /protein_id="HLJ71955.1"
                     /translation="MSDQEPGFSTLAIHAGARPDPTTGARATPIYQTTSFVFDDVDHA
                     ASLFGLQTFGNIYTRITNPTSAVLEERVAALEGGPAALAVASGHAAQVLVFHSLMQPG
                     DEFVASNKLYGGSINQFTHAFKNFDWRVKWADPDDIDSFRRALSDKTKAIFIESIANP
                     GGVITDIEAVAAIAKSANVPLIVDNTLASPYLIRPFAHGADIVVHSATKFLGGHGNSI
                     AGVIVDGGSFNWAGDKRYPMLSAPRPEYGGMILSETFGNFAFAIACRVLGLRDIGAAI
                     SPFNSFLVLTGIETLSLRMQRHSDNALRVAQHLKEHDAVSWVSYAGLPGDKYHDLARK
                     YCPKGAGGVMTFGVKGGYDAGVSLVRNLKLFSHLANIGDTRSLVIHPASTTHRQLTDA
                     QKTAAGAGPEVVRLSIGIEDAADLIADLDQALKAE"
     gene            7238..7894
                     /gene="aat"
                     /locus_tag="VKU03_11615"
     CDS             7238..7894
                     /gene="aat"
                     /locus_tag="VKU03_11615"
                     /EC_number="2.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265767.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008914 - leucyl-tRNA--protein transferase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl/phenylalanyl-tRNA--protein transferase"
                     /protein_id="HLJ71956.1"
                     /translation="MARPSERFEITPDILLRAYSIGLFPMAESADSSTLFWVDPKERG
                     IFPLDRLTVSKSLAKTVRADRFAVRADSVFDAVMAACAARDKTWINGDIRRLYGALFA
                     QKRAHSVEAYEGGALVGGLYGVSLGAAFFGESMFHTARDASKVALVHLVARLRFAGFK
                     LLDTQFVTPHLASLGAIEISRDAYIRRLDEALAAEADFDAWPPGRPMSGREALALARP
                     "
     gene            complement(7907..8443)
                     /locus_tag="VKU03_11620"
     CDS             complement(7907..8443)
                     /locus_tag="VKU03_11620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HLJ71957.1"
                     /translation="MMEERPIRYDATTIALHWATALSVVALWIIGQTADMLPHKIADG
                     FVWPVHVVWGFALVAIVAWRIVWRTAVGAGLPAAEPAFLHLIAKASHYLLYLLLAAVL
                     LLGVVNAFVRGYDLFGLFSLPQIGDKAWRRPITHWHELAANMLLIVALLHAAAALAHH
                     YVFKDGVLRRMLPAAEQI"
BASE COUNT         1453 a         2742 c         2868 g         1446 t
ORIGIN      
        1 ggcgatatga tgtttccgac ctatcgccag ccaggccttt tgatcgcccg cgaatggtcg
       61 ctctacgaca tggcctgcca agtcttcgcc aatgccggcg accggatgaa aggccggcaa
      121 ttgccggtgc tctattctgt gccgcaagag ggcttcttca ccatttccgg caatctggcg
      181 acgcagttca tccaagcggt cggctgggcc atggcctcgg cctataaggg cgacacgcgc
      241 atcgccgccg gctatatcgg cgacggcgcg acggcggagg gcgattttca ctacgccttg
      301 accttcgccg ccgtctatcg tgcgccggtc atcctcaatg tcgtcaacaa tcagtgggcg
      361 atctcgacgt tccagggctt cgccggcggc gagaacgcca ccttcgccga tcgcgccatc
      421 ggctatggcc tgccgggctt acgcgtcgac ggcaacgacc tgctggcggt gatcgccgcg
      481 acgcgctggg cggcggaacg ggcgcgcgcc aatttcggcg cgaccttgat cgagctcgtc
      541 acctatcgcg ccgccgccca ttcgaccagc gacgatccca gccgctaccg gccggccgac
      601 gagtgggagc attggccgct cggcgacccg attccgcggc tgaaacagca tctgatcgcc
      661 cgcggcgagt ggagcgaagc gcgccacacc cagctcggcg cggaagtcga ggacgaggtg
      721 cagagcgcgg cgaagaaagc cgaggctttg ggcgtgctgg gcaagggcgc gcagcatccg
      781 tcccaaacga tgttcgagga cgtctatcgg gagatgccgc cgcatctcaa gcgccagcgt
      841 caggaattcg aggccggccg atgagcgcaa tgaccctgtc gcaagcgctg aattcggcga
      901 tggacgtgat gctcggccag gacgacaacg tcctgctgat gggcgaggat atcggctatt
      961 tcggcggcgt cttccgctgc accgaaggct tgcagaagaa atatgggccg cgccgggtgc
     1021 tcgatacgcc gctcagcgag ggcggcattg tcggcgcggc gatcggcatg ggcgtctacg
     1081 gcttgcggcc ggtggtcgag atccagttcg ccgattattc gtaccccgct ttcgatcagt
     1141 tggcctcgga ggcggcgcgt ttgcgctatc gctcggctgg cgagttctgg gcgcccttga
     1201 ccgtgcgcac gccctatggc ggcggcattt atggcggcca gacccacagc cagagcccgg
     1261 aagccttgtt tacccatgtt tgcgggttga agacggtcgt tccgtccaat ccctacgacg
     1321 ccaagggctt gttgatcgcc gcgatcgagg aggacgatcc aatcatcttt ctcgagccga
     1381 agcgccttta tgccgggccg ttcgacggcc atcacgaccg gccggcgacg agctgggagg
     1441 gccatccgca tagcgacgtg ccggacggcc attatcgcgt gccgctcggc aaggcgaaga
     1501 tcgcgcggcc gggcaattcg gtcaccgtgc tcgcctatgg cacgatggtg catgtggcga
     1561 tcgccgcggc gcaagattgc ggcgtcgacg ccgaggtgat cgatctgcgc acgctgatcc
     1621 cgctcgatct cgacacgatc gtcgcctcgg tgaagaagac cggccgctgc gtcgtggtgc
     1681 acgaagcgac gctgacctgc ggctatggcg ccgagctcgt cgcgcttgtc cagcagcatt
     1741 gcttcttctg gctcgaaacg ccgatcgagc gcgtcgccgg ctgggacacg ccttaccctt
     1801 acgcgttcga atgggactat ttccccggcc ccaagcgcgt cggcgccgct ttgaagaaag
     1861 cggtggacgg caaggcatga aatacgtctt caagctgccc gatctcggcg aaggcacgac
     1921 caaggccgag atcgtcaagt ggcatgtcgc aaccggcgac atcgtccgcg aggatcagcc
     1981 gctggtcgac atgatgaccg acaaggcgac gatggagatc ccggcgccgg tcaacggcgc
     2041 gatcctggag ctgcatggcg agccgggcga cgttagagcg gtcggcgccg agctcgtggt
     2101 gttcgatgtc gcgagcgcgc cgcatgagcg cgcggcggag gccgcgccgc cgccgcgaca
     2161 agcggagccc gccgccgcag cgcggccgtt ggagaaagaa cggcctgttg aaaaacggca
     2221 ggccaagccg atcgcctcgc ccgtcgtgcg ccgccgcgcc gccgagcttg gcgtgtcgct
     2281 gtccgacgtg ccggcgagcg ggccggacgg ccgcgtcctg cgcgaggatc tcgaagcctt
     2341 cgccgccggc cggcggcggc ccgccgcggc ccgcagcgag gatcggcgcg aaggcgacat
     2401 tgaggagatc aaggtgatcg gcctgcgccg cgccatcgcc gagcgcatgc aggacgccaa
     2461 gcgccgcatt ccgcacatca cctatgtcga agagatcgat atttccgcgc tcgaagattt
     2521 gcgcgagcat ttgaaccgcg atctcgccga tcggcgcgtc aagctcacca ttctgccgtt
     2581 tctcattcgc gcgatggtca ggggggtcgc gcgctatccg caatgcaacg cgctgttcga
     2641 cgacgaagcc ggcatcgtca gacgccatcg cgccgtccat gtcgggattg cgacgcagac
     2701 ggcgtccggc ctgatggttc ccgtcttgcg ccatgccgag gcgctcgacc tcttcgaaag
     2761 cgctgaggaa attgcccgcc tcgccgagcg tgcgcgcgat ggcaaggcgg cgcgcgacga
     2821 gctcgtcggc tcgaccatca ccatatcgag cctcggcccg ctcggcggaa tcgcatcgac
     2881 gccggtgatc aatccgccgg aagtcgcgat tgtcggcgtc aacaagatcg tcgaaaggcc
     2941 ggtgattcag cgcggccaga tcgccgtgcg caagatgatg aatctctcgt cgtccttcga
     3001 ccatcgcatc atcgacggct gggacgcggc gtccttcatc caggcgatca aaggctattt
     3061 ggagcagccg gcgacgctgt tcatgaatga acacagcgat tgaacgctct gtcattccgg
     3121 cgtaagcggc gagtggcgcc tgcggcggaa tgacactgcg tacgtggact catgtgagcg
     3181 aatgcaaaga gcggcgaccg agtggcaagc taccttctaa ctcccggcgc gcggcgcatc
     3241 gccgtggccc agcctttgtc gggcatcggt gacatgatct ggcatgtgcc gcatatccgc
     3301 gcgctcgccc gccatgtcgg cgcgccggtg acgctgatcg ccagcttccg ctccgccgcc
     3361 gaccagattt tcaaggccga cgacgcggtc gcggacgtgc tcttcatcga gcgtcggccg
     3421 aaaggacgaa ccggccggca tgatggccct ctaggcatga tccgcctcgt ctcggcgttg
     3481 cgcgcgcgcc aattcgacgc actggtgctg ctgcatcatg gccgcacgct ggctttcgcc
     3541 gcgctttgcg ccggcgttcg gcagcgttca ggctatggcg tcggcctgca gcgcgccttt
     3601 ctcaatcgcg gaccgtacct tgccgccgac cgcttgcgcc tgcatccgtt cgagcgcgcc
     3661 tcggcctggc tggcggcggg cggcgtcgag ctcgccgaaa gcgagccgcg ccttgcgatc
     3721 gcgccggaca gcctggccaa ggcgcggctt ttcttgggcg aagccgaccc caagccggtg
     3781 gcgatcggca tcggctcttc ggagccgttc aagcaatggg gcgcggcgcg cttcgcggag
     3841 ctggcggaga aattgatcga agcgggatgg acgcgcctcg tcctgatcgg cggcaagccg
     3901 gagcgcccgc tggccgagga tatatgcgcg cgtcttggcg cgaaggccgg cggcgtcctc
     3961 gtctcgacgg ggtgggatat tgccgatgtc gccgctttgt tgagcctctc ggcattctat
     4021 gtcggcaacg acaccggctt catgaatatg gccgccgccg ccggcctgcg cagcttctgc
     4081 ctgttcggcg gcagcgcgcc gttccgccat agccgcaaca tcatgccgat cctgccgccg
     4141 gacggaaagc cggacaagaa tggcggcatg gcgcggatca cggccgcggc ggcgatggcg
     4201 gcgatcgcgg cgcaaggtct cgcgccgccg agggaagaag cgcgctacgg cgccggcgcc
     4261 gctggcctgc cgggcagaag cttgtagaac atcgtcgtct cggcgagccg cccatcgacg
     4321 cgataggcgt ggtccggcac ccggccatat tcgcgccagc cggcgctgcg atagagcctg
     4381 tcgccggaac tgccgctctc ggtgtcgagc aacagcaaag tacgtccggc ctggagcgcc
     4441 gcttcttccg cggcgccgag aaggcggcgg ccgaggccct gccggcgctc cgacgagaga
     4501 accagcatct tgccgatctc ggcgcgatgc ggcgcattgg gctgcggcgc atagatcaga
     4561 aggacggtgc cgacgagccg gccgccccgc tcgccgacaa aaagccgctt cccgccgccc
     4621 gcaatgtcgt cgatctgcct gcgccagaac gcgcgtccgt cgtcccgagc gaagccggcg
     4681 aggaaattga ccgacgcgcc atgcgcgacc gcgtcgacga ggatgtcggc gagctcggtc
     4741 aggcgcgctt cggtctcggc cgcgtccaac tcgcggatcg acgtttggct aagcatccga
     4801 ttgtccccgt ccatgcctta gctccggctg agaacgacgg cgtagcgggc tgggcgcgac
     4861 gacggattgc gaaatcggac cggcttgccg aaccgcatgt aaaagcagtc gcccgcctcc
     4921 agccggacta tctctccatc gagatcgatc tcgatcgcgc cgtcgagcag ccagatgtgc
     4981 tgatccttgc cggcgatacg gtcattggcg aaggcgacgc tcgcgccggg cggaaactcg
     5041 atttcgacaa ggtcgattgc ggagccggtc gcggcggggg agacgttgcg ccggacatag
     5101 ccgctcgccg gatcgcgcca ggccggctga tcggcgcggc ggcaaagcgg gctcgccggc
     5161 ttcccgcttt cggcgaagag ctccgacaac gtgatcctga gcccgccgca gatcttgttg
     5221 aggagctgcg ccgtcgggct cgattcgccg cgctcgatcc tcgacagcat ggcgcggctg
     5281 acctcggcga ggctcgccag gccctctaaa gtgaggccgc gctcggcccg caaggcgcgg
     5341 atgcgggcgg caaggcgggc gtcgatatcg gattcggcga ggctcacata tgagctatat
     5401 tatgttatta tattggaatc aagctttttg gagaccaact atgataaatc cgcgcgtgag
     5461 gaagataacc aatggcgcag cagccgatgt cgctcatcga cgcaatcgtc acggggctga
     5521 agcgccgcgc gccgaggcgt cgaaagcaag atgagaccag tgatgggttc gccgcgcgct
     5581 ctgcgcgact acggtccgct aaacccgacc gtttcaaggt agattccgcg gaactgatca
     5641 gaccggaccg cgatgaacga taagcgagag tggcgagcct ctgcgacgag gatacgctgt
     5701 cattgcgagg accccgagcg cagcgagagg gatgaagcaa tccgtgggcg cctgcttcag
     5761 cccaagcgcc gcttggattg catcgcttcg cgcgcaatga cggtagcgct cactccgcct
     5821 tgagcgcctg gtccaaatcc gcgatcaaat ccgccgcgtc ctcgatcccg atcgacaggc
     5881 gcacgacttc cggcccggcg cccgccgcgg tcttctgcgc gtcggtgagc tggcgatggg
     5941 tggtcgaggc gggatggatc accagcgagc gggtgtcgcc gatattggcg agatgcgaga
     6001 agagcttgag gttcctcacc agcgacacgc ccgcgtcgta gccgcccttg acgccgaacg
     6061 tcatcacgcc gcctgcgcct ttcgggcagt atttgcgggc gaggtcgtgg tacttgtcgc
     6121 cgggcaggcc ggcataggag acccaggaga cggcgtcgtg ctccttcaaa tgctgtgcga
     6181 cgcgcagagc gttgtcgctg tggcgctgca tgcgcaagct cagcgtctcg atgccggtca
     6241 ggacgaggaa cgagttgaac ggcgaaatgg cggcgccgat gtcgcgcaga cccagcacgc
     6301 ggcaggcgat ggcgaaggcg aaattgccga acgtctcgga gaggatcatg ccgccatatt
     6361 ccggccgcgg cgccgacagc atcggatagc gcttgtcgcc cgcccaattg aacgagccgc
     6421 cgtcgacgat gacgccggcg atcgagttgc cgtggccgcc gaggaacttg gtcgccgaat
     6481 ggacgacgat gtcggcgcca tgggcgaaag gccggatgag gtaagggctc gccagcgtgt
     6541 tgtcgacgat cagcggcacg ttggcgctct tggcgatcgc ggcgaccgct tcgatatcgg
     6601 tgatgacgcc gccgggattg gcgatcgact cgatgaagat cgccttggtc ttgtcgctga
     6661 gcgcgcggcg gaaggaatcg atatcgtcgg gatcggccca tttcacccgc cagtcgaaat
     6721 tcttgaaggc gtgggtgaac tgattgatcg agccgccata gagcttgttc gaggcgacga
     6781 attcgtcgcc cggctgcatc agcgaatgga agacgaggac ttgcgccgca tggcccgacg
     6841 ccacagcgag ggcggccggc ccgccctcca aggccgcgac gcgctcctcc agcaccgcgc
     6901 tcgtcggatt ggtgatgcgc gtatagatgt tgccgaacgt ctgcaggccg aacaacgagg
     6961 cggcgtgatc gacatcgtcg aaaacgaagg aggtcgtctg gtagatcggc gtcgcccgcg
     7021 cgccggtggt cggatcgggc cgggccccgg catggatcgc gagggtggag aatccgggtt
     7081 cctggtcgct catttttctc atcctctccc ggcttgaccg ccggtcggcg ctttgcagcg
     7141 cccatcttca tcaggatagg cggaaaggca atcttgcgcc tcgccgcgcg ccgccgcttg
     7201 cttgacggcg ggcgaccgcg gcagcactgt cgccggcatg gcgcgtccga gcgagcgttt
     7261 tgaaatcacg cccgatattc tgctgagggc ctattcgatc ggcctgtttc cgatggccga
     7321 gagcgcggac tcctcgaccc tgttctgggt cgatcccaag gagcggggga tttttccgct
     7381 ggaccgtcta acggtctcca aaagcctcgc caagacggtc cgggcggacc gtttcgcggt
     7441 tcgggccgac agtgtattcg acgccgtcat ggcggcttgc gccgctcgcg acaagacctg
     7501 gatcaacggc gacatccgcc ggctctatgg cgcgcttttc gctcaaaagc gggcgcattc
     7561 ggtcgaggct tacgagggcg gggccctggt tggcgggctc tacggcgttt cgctcggcgc
     7621 cgcctttttc ggcgagagca tgttccacac cgcccgcgac gcctcgaaag tggcgctggt
     7681 tcacctcgtc gcgcgcctgc gcttcgccgg cttcaagctg ctcgacacgc aattcgtcac
     7741 gccgcatctc gccagcctcg gcgccatcga gatttcccgc gacgcctata tcaggcgtct
     7801 cgacgaggcg ctcgcggctg aggccgattt cgacgcctgg ccgccgggcc ggccgatgag
     7861 cggacgcgag gcgctggcgc tcgcgcgacc ataggacttt ggcccttcaa atctgttcgg
     7921 ccgccggcaa catgcggcga agcacgccat ccttgaagac atagtgatgg gccaaagcgg
     7981 ccgccgcgtg cagcagcgca acgatcagca gcatgttcgc ggcgagttcg tgccaatgcg
     8041 tgatcggccg ccgccacgcc ttgtcgccga tctgaggcag gctgaacaag ccgaagagat
     8101 cgtagccgcg cacgaaggcg ttgacgacgc cgagcagcag cacggccgcc agcaagaggt
     8161 agaggaggta atggctcgcc ttggcgatca ggtggaggaa ggccggctcg gcggctggca
     8221 gaccggcgcc gacggcggtg cgccagacga tgcgccaggc gacgattgcg accagcgcga
     8281 agccccagac cacatggacc ggccaaacga atccgtcagc gattttatgc ggcagcatgt
     8341 cggccgtttg gccgatgatc cagagcgcaa cgacggaaag cgcggtcgcc caatgaaggg
     8401 cgattgtcgt tgcatcgtag cggatcggtc gttcttccat catagtcccc tcgcaggaaa
     8461 tttgaaggcg tggcgccaag cggccatcga gaatcgcggg ttctcttta
//
LOCUS       DATGJW010000385         3522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1520540, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000385 DATGJW010000000
VERSION     DATGJW010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3522
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1520540"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..581
                     /gene="serB"
                     /locus_tag="VKU03_11625"
     CDS             <1..581
                     /gene="serB"
                     /locus_tag="VKU03_11625"
                     /EC_number="3.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029151.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036424 - L-phosphoserine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HLJ71958.1"
                     /translation="AGIGARVAAITERAMRGEIAFEPALRERVGLLAGLPATIVGELL
                     ETRIALSPGARALVQTMRKAGAYTALVSGGFQQFTGAIAARLGLHEHRANLLIVEDGK
                     LTGKVAEPILGRDAKLATLRDLAARQGLALAETLAVGDGANDLAMLDAAGLGVAYRAK
                     PAVAAAAHARIDHADLTALLYAQGYRREEFAE"
     gene            841..2052
                     /locus_tag="VKU03_11630"
     CDS             841..2052
                     /locus_tag="VKU03_11630"
                     /inference="COORDINATES: protein motif:HMM:TIGR00843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0042925 - benzoate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0042919 - benzoate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="benzoate/H(+) symporter BenE family transporter"
                     /protein_id="HLJ71959.1"
                     /translation="MQAFTPDFLMQQPSAFHAIIAGLVAAFVGFASSFPVVIQGLKAI
                     GASDAQAASGLMALSIAMGVAGIILSVATRLPISVAWSTPGAALLASTGAIQGGFSAA
                     VGAFLIVALLLIAAGLFKPLSRAVAAIPAPLANALLAGVLFSLCLAPVKAMAETPAAA
                     AAIIVVWLIVARFKRLYATPAAAIIALGLILWSNHGAPIGWADLAPRPLAIAPSFSLS
                     AMISVALPLFIVTMASQNIPGLAVLSASGYRPNPGPLIAVTGFFSLLAAPFGGHAVNL
                     AAITAALCASPDAHPDAGKRWIASAANGAIYIVFGLVAGAVTKAVAGAPILIEAVAGL
                     ALLGAFASSLGAALAEPDSREAALATFLAAASGLSFFGIGGAFWGLVAGGAILLLNRA
                     GAKRRPLKPTS"
     gene            complement(2006..3055)
                     /gene="speB"
                     /locus_tag="VKU03_11635"
     CDS             complement(2006..3055)
                     /gene="speB"
                     /locus_tag="VKU03_11635"
                     /EC_number="3.5.3.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008783 - agmatinase activity [Evidence
                     IEA];
                     GO_process: GO:0008295 - spermidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="agmatinase"
                     /protein_id="HLJ71960.1"
                     /translation="MVDKAKLETLRDLYRDAKGGDMHDPHFRKVAEKLFQGGDRRKWP
                     FAGVATFLDAPYRPDAPSLADFGGLDIALVGVPMDLGVTNRAGARLGPRAVRAVERMG
                     PYEHVLKSAPLVEARVADIGDVPMRSRYSLESCHEDIEAFYTRLVGAGIVPLSVGGDH
                     SIVHPILKAVGAERPVGLLHIDAHCDTGGPYEGSKFHHGGPFRQAVLDGVLDPERAIQ
                     IGIRGGAEYLWEFSYASGMTVIHAEELAATGVDKVVAKAREVIGAGPAYLSFDIDSLD
                     PGFAPGTGTPEVGGLTPREALAIVRGCHGLNFVGGDVVEVAPQYDATTNTAQCAAQML
                     FTMSALMVAALRRRG"
     gene            3279..>3522
                     /locus_tag="VKU03_11640"
     CDS             3279..>3522
                     /locus_tag="VKU03_11640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267540.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TerC family protein"
                     /protein_id="HLJ71961.1"
                     /translation="MSGFHLTWGFLGAAIELAAVDLLLGGDNAILIALASRVLPPDLR
                     RRAMIYAVCGAIALRFIFAGATGFLLRLPVIKLVGGL"
BASE COUNT          507 a         1199 c         1178 g          638 t
ORIGIN      
        1 gcgccgggat cggcgccagg gtcgcggcga tcactgagcg cgcgatgcgc ggcgaaatcg
       61 ccttcgagcc ggctctgcgc gagcgggtcg gcctgctcgc cggactgccg gcgacgatcg
      121 tcggcgagct tctcgaaacg cgcatcgcgc tttcgcccgg cgcgcgcgcg ctggtccaga
      181 ccatgcgcaa agccggcgct tacaccgcgc tggtctcggg cggctttcag cagttcaccg
      241 gcgccatcgc cgcccggctc ggcctgcacg agcatcgcgc caatttgctt atcgtcgaag
      301 acggaaagct gaccgggaag gtcgccgagc cgatcctcgg ccgcgacgcg aagctcgcga
      361 ctttgcgcga tttggccgcc cgccagggcc tcgcgctcgc cgagacattg gcggtcggcg
      421 acggcgccaa cgatctggcg atgctcgacg ccgccggcct cggcgtcgcc taccgcgcca
      481 agccggcggt cgccgccgcc gcccacgccc gcatcgacca cgccgacctg acggcgctcc
      541 tctacgcgca ggggtacagg cgggaggaat tcgcagagta gggatgtgac atgagtcgga
      601 aagatcgccg cgctgcgcgc ggcgctaagc aagtcttcgt cgtcgaggcg gcgacaggaa
      661 aaagggcgac gccgttgtct gaatgcttcg ctgctatcgc acgcgagttg cgcgccaagg
      721 ctggtcccaa gggagatgac atgagtcgcg aagagatcgc tgcgatgtgg ggccattgat
      781 gttgatccag actgttcgct ttctttgcaa tgcggtttcc tgtctgacgc gggttaggta
      841 atccaagcgt tcacgccgga ttttcttatg cagcagccct ccgcctttca cgcgatcatc
      901 gccggcctcg tcgccgcctt tgtcgggttc gcctcctcat tcccggtcgt catccagggc
      961 ctcaaggcga tcggcgcgag cgatgcgcag gcggcgtcgg ggctgatggc gttgtcgatc
     1021 gcaatgggcg tcgctgggat catcctcagc gtcgcgacgc gcctgccgat ctccgtcgcc
     1081 tggtcgacgc cgggcgcggc tctgctcgcc tcgaccggcg cgatccaagg cgggttttcc
     1141 gcggcggtcg gcgcttttct catcgtggcc ttgctgctga tcgccgccgg cttgttcaag
     1201 ccgctgagcc gggcggtggc ggcgattccg gcgccgctcg ccaatgcgct gctggccggc
     1261 gtgttgttct cgctctgcct cgcgccggtc aaagccatgg ccgagacgcc cgccgcggcg
     1321 gcggcgatca tcgtcgtctg gctgatcgtc gcgcgcttca agcgcctcta cgcgacgccg
     1381 gcggcggcga tcatcgcttt gggtttaatc ttatggagca atcacggcgc gccgatcggc
     1441 tgggccgatc tcgcgccgcg cccgctcgcc atcgcgccga gtttttcgct cagcgcgatg
     1501 atcagcgtcg cgctgccgct tttcatcgtc acgatggctt cgcagaacat tcccggcctc
     1561 gccgtgcttt cggcttccgg ctaccggccc aatcccggcc cgctgatcgc agtcaccggc
     1621 tttttcagcc ttctcgccgc gccgttcggc ggccacgcgg tcaatctcgc cgcgatcacc
     1681 gccgcgctct gcgcctcgcc cgacgcgcat cccgacgcag gcaagcgctg gatcgcctcg
     1741 gccgcgaacg gcgcgatcta tatcgttttc ggcttggtcg ccggcgcggt gaccaaagcg
     1801 gtggcgggcg cgccgatcct gatcgaggcc gtggccggct tggcgctgct cggcgctttc
     1861 gcctcctcgc tcggcgcggc gctcgccgag cccgattcgc gcgaggcggc tttggcgaca
     1921 ttcctcgccg ccgcttctgg cctgtccttt ttcggaatcg gcggcgcgtt ctggggtctc
     1981 gtcgcgggcg gcgcgattct cctgctcaac cgcgccggcg caaagcggcg accattaaag
     2041 ccgacatcgt gaacagcatc tgcgccgcgc attgggcggt gttggtcgtt gcgtcatatt
     2101 gcggcgcgac ctcaacgacg tcgccgccga caaaattgag gccatggcag ccgcggacga
     2161 tggcgagcgc ctcgcgcggg gtcaggccgc cgacttccgg cgtgcctgta cccggcgcaa
     2221 agccgggatc gagactgtcg atatcgaaag aaaggtacgc cgggccggcg ccgatcacct
     2281 cgcgcgcttt ggcgacgacc ttgtcgacgc cggtcgcggc gagctcttcg gcatggatga
     2341 cggtcatacc ggaggcgtag gagaactccc agagatattc cgcgccgccg cgaatgccga
     2401 tctggatggc gcgctcggga tcgagaacgc cgtccagcac ggcttggcgg aacggcccgc
     2461 cgtgatggaa tttcgagcct tcatagggcc cgccggtgtc gcaatgcgcg tcgatgtgaa
     2521 gcaggccgac cggccgttcg gcgccgaccg ctttcagaat gggatggacg atcgagtgat
     2581 cgccgccgac gctgagcgga acgattcccg cgccgacgag ccgcgtatag aacgcctcga
     2641 tgtcctcgtg acagctttcc agggaatagc gcgagcgcat cggcacgtcg ccgatatcgg
     2701 cgacgcgcgc ttcgaccagc ggcgccgatt tgaggacatg ctcatagggg cccatgcgct
     2761 cgaccgcccg cacggcgcgg gggccgagcc gggcgccggc gcgattggtg acgccgagat
     2821 ccatcggcac gccgaccagc gcgatgtcga gcccgccgaa atcggcgagg gacggcgcgt
     2881 ccggccgata gggcgcatcg agaaaggtcg cgacgccggc gaacggccat ttgcggcggt
     2941 cgccgccttg aaacagcttc tccgcgactt tgcggaaatg cggatcgtgc atgtcgccgc
     3001 ctttggcgtc gcggtagaga tcgcgcaaag tctcgagctt ggccttgtcg accatcgcga
     3061 atctcctgga agaaatcgca gcggataatg ggtagcacaa agaatagcgg cggagcattc
     3121 ccttctcccc gctcgccgct tgcggggaga aggtggcccg aacggccgga tgaggggccg
     3181 cgccggactc gaaagtacgc gctcccggcc gagtttcctc acccgatcgt catattctac
     3241 tcttgccttc gacgacaaag acccttctaa gtccgctcat gtcgggcttt cacctgacct
     3301 ggggctttct cggcgcggcg atcgagctgg cggcggtcga tctgctgctg ggcggcgaca
     3361 atgcgatcct gatcgcgctc gcctcgcgcg tgctgccgcc tgatttgcgc aggcgcgcca
     3421 tgatctatgc ggtctgcggc gcgatcgctc tgcgcttcat cttcgccggc gcgacgggat
     3481 tcctccttcg cctgccggtc atcaagctcg tcggcggcct cg
//
LOCUS       DATGJW010000386        13489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_385994, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000386 DATGJW010000000
VERSION     DATGJW010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 13489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13489
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_385994"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..183
                     /locus_tag="VKU03_11645"
     CDS             <1..183
                     /locus_tag="VKU03_11645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase"
                     /protein_id="HLJ71962.1"
                     /translation="LLTLAIVLARRAPLPAWAERLDWVARLHHPGNGVPYGVALAAAG
                     LALYPSSQIWRAATGL"
     gene            328..1134
                     /gene="cpaB"
                     /locus_tag="VKU03_11650"
     CDS             328..1134
                     /gene="cpaB"
                     /locus_tag="VKU03_11650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007565969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Flp pilus assembly protein CpaB"
                     /protein_id="HLJ71963.1"
                     /translation="MNKAQIAVLGLTIVAFGGAYVMFASSGGPPVPQVIKVTQKVDTD
                     EVLVAAHDLQMGTKLTDADYRWQEWPKAALSPLMIKKSDGQAAADEVKGAVTRSDFLQ
                     GDPMRRDKIITGASSGFLSAILPSGMRAVAINIDSQGSTTAGGFILPNDRVDVIQLYR
                     DDEASKARGSEVWTPRTILTNVRVLAIGQNVQEKNGERVVVGSNATLELDPTQVEQII
                     LAQRAGNGNLHLALRSLLDSKGKTESNVSENTNEGGLTIVRFGAAQEAAR"
     gene            1175..2704
                     /locus_tag="VKU03_11655"
     CDS             1175..2704
                     /locus_tag="VKU03_11655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015951036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II and III secretion system protein family
                     protein"
                     /protein_id="HLJ71964.1"
                     /translation="MNRARIKGPALLWLAAAAALFPFAPAATAPAWSAEQGVAAWQPP
                     APVARRLQLGVGKSVIVDLPEEAGEIFVGNPQVANAIVRSARRLYIDAIANGQTTIFA
                     MDRQGRQIAVVEISVGRDVGELQALLNAAMPGNDIRVKTVADSVILMGSVASAAEAQK
                     ALDIASGFVGTSVLGGGPSVAAPSGGGNSTQVSISASGAPVTGKVINSLTIRGADQVA
                     LRVTVSEIRRDIVKQLGVNLFGQSASGSSFQVTNPFAINGAISATNALLNWSKGGNTF
                     QGQLLAFERQGVARILAEPTVTAISGESAKFLAGGTIPIANGLSCTTGTVVVASQCLL
                     SILQQPYGVSLNFTPVVLSQGRIQLRIATEVTDIDFSKQITLSEAGTNVSVPGFRTRK
                     NETTVELPSGGSIASAGLLSTETSQAINGVPGLMNLPILGALFRSRDFQRNETELVII
                     VTPYIVHAIDPGQVVKPDANFADATDPQTWLLGRVNKLYSTAGTRGLQNYSGKIGFIN
                     E"
     gene            2751..3476
                     /locus_tag="VKU03_11660"
     CDS             2751..3476
                     /locus_tag="VKU03_11660"
                     /inference="COORDINATES: protein motif:HMM:NF021025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaD family pilus assembly protein"
                     /protein_id="HLJ71965.1"
                     /translation="MAATGSARKAKRLGLLALGFLGAAALGGCHDDDVITGSIAPDDY
                     HNRHPIVLTDGPTTLDVFPTGQSALDERSAANVRAFAERYAKYGVGRIVLLAPAGGGP
                     KIHAGMNAIRRELAAAGLRGRIGIGSYPVADPLLAAPIKLSFVGLKAEVPPHCGQWPV
                     DLASGSSLETWKNETYWNYGCATQSILAAEVDNPRDFVQAGALGPSDVQMRLRAINAV
                     RNGTDPGTGWNVKNTSIGQIGGS"
     gene            3480..4763
                     /locus_tag="VKU03_11665"
     CDS             3480..4763
                     /locus_tag="VKU03_11665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HLJ71966.1"
                     /translation="MSVDFNGVGPAGATQDAPNPPQINPVPRISIQAFCETQEVAAVI
                     QAAMSDRRMNKAHVKLHMGGPAAAVEAYRSAPTPNVIVLESSQDRDALTVSLDELAEF
                     CDAGTKVIVCGKINDIVLYRHLILKGVSEYLVAPFSVLDFVQAISNLYNSPGANPVGR
                     VIAVTGAKGGVGASTIAHNLAWSVATGLETSTVIADMDLGFGTAGLDYNQDPPQGMAE
                     AVFAPDRVDSNLVERLLSKCADNLSLLAAPATLERPYDFGETAFDAVIDILRASTPCV
                     VLDVPHIWSAWTKRALVGADDVIVVAGPDLANLRNAKNLLDNLRGARPNDRSPKLILN
                     GVGVVKRPEIAVADFAKTVEVEPAAVIPYDAKLFGAAANNGQMIAEIEANGKTAAIFA
                     ELARELNGKLELKKAKRSLFDPLISALGRKKARAA"
     gene            4803..6230
                     /locus_tag="VKU03_11670"
     CDS             4803..6230
                     /locus_tag="VKU03_11670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HLJ71967.1"
                     /translation="MFGKRASGANAAAAPPSAPARPLESAPAEAAPRASAPEPVRAAA
                     EPRRSDSYYQTKSMIFGALIEAIDLSALAKLDPDNAREEIRDIVHEIVTIKNIVLSIA
                     EQEDLLEDICNDVLGYGPLEPLLARDDIADIMVNGPTRCFIEVGGKIQLTNVRFRDNA
                     QLMNICQRIVSQVGRRVDEASPICDARLPDGSRVNVIAPPLAIDGAALTIRKFKKDKL
                     TLDQLTRFGSISPAGAEILKIIGRVRCNVIISGGTGSGKTTLLNCLTNYIDVDERVIT
                     CEDAAELQLQQPHVVRLETRPPNLEGAGQITMRDLVKNCLRMRPERIIVGEVRGPEAF
                     DLLQAMNTGHDGSMGTLHANSPREAMSRIESMITMGGYALPSRTIREMIVSSIDVIIQ
                     AARLRDGSRRITHITEVMGMEGDVIITQDLFVYELLGEDAQGKLKGRHRSTGIGRPRF
                     WERARYFGEEARLAAALDASEVKAA"
     gene            6241..7200
                     /locus_tag="VKU03_11675"
     CDS             6241..7200
                     /locus_tag="VKU03_11675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLJ71968.1"
                     /translation="MTSNPFLLAVFLVIALGGGALVLVSGDSRAKKRQDALKKTTVKM
                     RDAGADKNARRKQVADSLKELEQRTGRKKATLQARLQQAGLALTPQQFYIVSALGAAF
                     VALAIFFVTRSLFIAAPSAIIGGFGAPNFVLNFLRKRRIAKFVAEFPNAIDIIVRGVK
                     AGLPIGDCLRVVANESVEPVRGEFRGIVEAQALGLSIGEAVDRLAERVPVTEANFFAI
                     VINIQAKAGGNLSEALGNLSRVLRDRKKMRGKISAMSMEAKASAAIIGCIPFVVTGLL
                     YLSSPKYVSLLWTTSHGRIIAAIGLVWMGIGTMMMKRMVSFDF"
     gene            7219..8196
                     /locus_tag="VKU03_11680"
     CDS             7219..8196
                     /locus_tag="VKU03_11680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HLJ71969.1"
                     /translation="MFDLIIEKLSNPHFVAGLLVAVGCAASVLTLAMPLLQTDNLGRR
                     MKAVSSERERIRQRERERLTTGQQKPSLRQEPKRLFKQVVDQLNLSTWLGTEKAKQQL
                     SMAGYRGAGAEYTFLFFRLVTPILFFCVTVFYVFVVLHWDKPLVMKIGVAIGAAYLGI
                     KGPEIFLSNTIAKRKKSLERAYPNALDLLLICAESGMSIELAVRKVAQEIGVESVPMA
                     EELTLLAAEMSFLSDRRQAYENLGARTGLESVRALVTVLIQAERYGTPLGTALRVLAQ
                     ESRDQRMTAAEKKAAALPPQLTVPMVLFFLPGLFVAIMTPAIIQLKHWT"
     gene            8366..10630
                     /locus_tag="VKU03_11685"
     CDS             8366..10630
                     /locus_tag="VKU03_11685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLJ71970.1"
                     /translation="MSARARAAFAPRYLEGLNPAQREAVEALDGPVLVLAGAGSGKTR
                     VLAVRIAHLIASGRARPHEILAVTFTNKAAREMRERISVLTGVEEGMPWMGTFHSIGA
                     KILRRHAELVGLQPNFTILDTDDQIRLLKQILQAENIDDKRWPARALAGQIDAWKNRG
                     LDPAHVSAAESAAFANGRGAALYAAYQARMKILNAADFGDLLLEGLRLFREREDVLKR
                     YQERFRYILVDEYQDTNVVQYLWLRLLAQARRNLCCVGDDDQSIYGWRGADVDNILRF
                     EKDFPGAKVIRLERNYRSTGHILAVASHLIAHNKGRLGKTLFTEAEDGDKPTVACAED
                     SQEEARVVGEEIEALQRRGQSLDEIAILVRASFQMREFEERFITLGLPYKVIGGPRFY
                     ERQEIRDALAYLRCVAQPDDDLAFERIFNVPRRGLGEATLTLLHDYARRAGLSLMRAA
                     RVMIESEELKARPRQILRDLVQSFDRWSAMVEKTPHEELAQTVLEESGYIDMWKAERT
                     ADAAGRLENLKELTRSMEEFPSLAAFLEHVSLVLDAESDEQGERVSIMTLHAAKGLEF
                     DVVFLPGWEEGLFPHQRSLDENGRAGLEEERRLAYVGLTRARLQAKIYFAANRRIRGL
                     WQSTQPSRFLDELPADHVEVLEAGGAPAYPASRFDRVEPFASTYESPGWRRAQARANA
                     PGAAAARRAPVTIEGRLMAQSAVESRFAKGARVFHIKFGPGSVAAVDGAKLTVDFDKA
                     GRKMVLESFVQPAD"
     gene            10717..12258
                     /gene="murJ"
                     /locus_tag="VKU03_11690"
     CDS             10717..12258
                     /gene="murJ"
                     /locus_tag="VKU03_11690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906639.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HLJ71971.1"
                     /translation="MFRSILSVGGFTLLSRLTGFARDLALGALLGAGPIADAFVVAFR
                     LPNYFRAIFGEGAFNTAYVPSYARVLETEGGGEAKHFASQVFTILFVSQIVLLVFAFA
                     FTPLLVRVIAPGFEADPEKFALAVRMTRITFPYLFFITLATLHSGTLNAHGRFAVAAF
                     APVLLNLTMLAFLAVAFRFPNAGEAASWGVAVSGALQLALLMLAARRRNLLEGFARPR
                     LSADIRQFFGALAPAVIGSAGVQIALLADSIIASMLPTGAVASIYYAERLYQLPIGVV
                     GVAAGTVLLPQMSRRLASGDKEGAARAQNRTAALTLALSAPFFVAFILVPEPIMRGAF
                     LRGAFTAADAARSAAVLAAYGIGLLPIVLIRSAVASFQSRGDTMTPMFVSLGAIAVNV
                     ALKFVFYRSLGAAGLALATAIGAWLNLAGLTMIALARGLMRPDTHLGEVAAAACCASG
                     VLALALSVSLPAAAQFAATAPHWRDEILLVILAGIGAAAYVLALLAGLGALGFRFRQA
                     RAQSS"
     gene            complement(12246..>13489)
                     /locus_tag="VKU03_11695"
     CDS             complement(12246..>13489)
                     /locus_tag="VKU03_11695"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71972.1"
                     /translation="AQLKHLVNQRTAAMQDAPDFNDADNSQIAAPDMAADKAPLAESA
                     ASEAPSAAAGQAGAEARPEPIEIRANAGASAEPPAREAPPVEPARATLIPFVAPRRQG
                     SALGALGRFAADRRFQTAAAAACLALVAIVAGGATLERAAKSERESQRLAQAVSAMSA
                     RIAAVETARPRDETAEIRKAVSEARGGLATSRDLSATIAQLNARLDRLEREHQARLDK
                     LGDRLEREVAARGAESQTRSADLAQRLDRLEKTDVAARLDKLEKSDLAARVDKLEKAD
                     LSARIDKVEKKVAAQTASAAAPLPPQKAAAASPGVSNEVTGSIERPHPTEPIRGWYLI
                     EMRNGSAVVENRQGMHQIAPGDSLPGAGKVERFEKRGREWVVVTDQGVIVQAAAGSYA
                     PRVVLRPPAYGPYGPGYDYED"
BASE COUNT         2173 a         4604 c         4377 g         2335 t
ORIGIN      
        1 ctgctcactt tggccatcgt gctggcgcgg cgggcgccgc tgccggcctg ggcggagcgc
       61 ctcgactggg tcgcccggct gcatcaccca ggaaacggcg tcccctatgg cgtcgccctc
      121 gcggcggcgg gcttggcgct ctatccgagc agccagattt ggcgcgccgc gaccgggctc
      181 taaggcgcgc ggaaacaccc ccggaagccc gcccgggcgg attagatacg tctggttaac
      241 cctaatttga cgattgacgg ttcaaatggg gccaaatcgg cgtgcaaagg cgcgtcggcg
      301 tcaggggcgc ggtgagggtt tggcgttatg aacaaagctc aaatagcggt ccttggcttg
      361 acgatcgtcg cctttggcgg cgcttatgtc atgttcgcgt ccagcggcgg cccccccgtg
      421 ccgcaagtca tcaaggtgac tcagaaagtc gataccgacg aggtcctggt cgccgcccat
      481 gatctccaga tgggcaccaa gctcaccgac gcggattacc ggtggcagga atggccaaag
      541 gccgccctgt cgccgctgat gatcaagaaa tccgacggcc aagccgccgc cgacgaggtc
      601 aagggcgcgg tgacgcgctc ggatttcctg caaggcgatc cgatgcggcg ggacaagatc
      661 attacgggcg ccagttccgg cttcctgtcg gcgatcctgc cgagcggcat gcgcgcggtc
      721 gcgatcaata tcgactcgca aggcagcacc acggcgggcg gcttcatcct gccgaacgat
      781 cgcgtcgacg tgatccagct ctatcgtgac gacgaggcct ccaaggcgcg cggctcggaa
      841 gtctggacgc cgcgcaccat cctcaccaat gtccgcgtcc tggcgatcgg ccagaacgtc
      901 caggagaaga acggcgagcg cgtcgtcgtc ggctccaatg cgacgctgga gctcgatccg
      961 acgcaggtcg agcagattat cctcgcgcag cgcgccggca acggcaatct ccacctggcc
     1021 ctgcgcagcc tgctcgattc caagggcaag accgaatcca acgtgtctga aaatacgaac
     1081 gagggcgggc tgaccatcgt tcgcttcggc gccgcgcaag aggcggctcg atgagcggcg
     1141 tttggcgcga tcggccagat tggggtttca ctggatgaac cgtgcacgga tcaaaggccc
     1201 cgctctgttg tggctcgcgg ccgcggcggc gctctttccc ttcgcgccgg cggcgaccgc
     1261 gccggcctgg agcgccgagc agggcgtcgc ggcgtggcag ccgccggcgc cggtcgcccg
     1321 ccggcttcag ctcggcgtcg gcaagtcggt gatcgttgac ctgccggagg aggccggcga
     1381 aatatttgtc ggcaatccgc aagtcgccaa cgcgatcgtc cgctcggcga ggcgcctcta
     1441 tatcgacgcg atcgcgaatg gccagacgac catcttcgcc atggatcgcc agggccggca
     1501 gatcgccgtc gtcgagattt cggtcggccg cgatgtcggc gagcttcagg ctctgttgaa
     1561 cgccgccatg cccggcaacg acattcgggt caaaaccgtc gccgattccg tgatcctgat
     1621 gggatcggtc gcctcggcgg ccgaagcgca aaaggcgctc gacatcgcca gcggttttgt
     1681 cggcacgtcc gtgctcggcg gcggcccgag cgtcgccgcg ccgagcggcg gcggcaattc
     1741 gactcaagtc agcatcagcg cgagcggcgc gccagtcacc ggcaaggtga tcaactcgct
     1801 cacgatccgc ggcgctgacc aagtcgcctt gcgggtgacg gtctccgaaa tccgccgcga
     1861 catcgtcaag cagctcggag tcaatctctt cggccagagc gcctcgggca gctcgttcca
     1921 ggtgaccaat cccttcgcga tcaacggcgc gatttccgcg accaacgcgc tgttgaactg
     1981 gagcaagggc ggcaacacct tccaggggca attgctcgct ttcgagcgcc agggcgtcgc
     2041 ccgcatcctg gccgagccga ccgtcaccgc gatttccggc gaatcggcga agtttctcgc
     2101 cggcggcacg atcccgatcg ccaacggctt gagctgcacg accggcacgg tcgtcgtcgc
     2161 cagccagtgc ctgctcagca tcctgcagca gccttatggc gtgtcgctga acttcacgcc
     2221 ggtcgtgctc tcccaagggc gcatccagtt gcgcatcgcg accgaagtca ccgatatcga
     2281 cttctccaag cagatcacgc tcagcgaagc gggcaccaac gtttcggtgc cgggcttccg
     2341 cacgcgcaag aacgagacca cggtcgagct tccgtcgggc ggctcgatcg cctcggccgg
     2401 cctgctctcg accgagacgt cgcaggcgat caacggcgtt ccgggcctga tgaacctgcc
     2461 gatcctcggc gctttgttcc gctcgcgcga cttccagcgc aacgagaccg agctcgtcat
     2521 catcgtgacg ccgtacatcg tccacgcgat cgatcccggc caggtcgtca agcccgacgc
     2581 caatttcgcc gacgccaccg acccgcagac ttggctgctc ggccgggtga acaagctcta
     2641 ctccacagcc ggcacgcgcg gcttgcagaa ttattccggc aagatcggct tcatcaatga
     2701 atagcccaat ctcgtcgccg cctcgcggcg cttcacggag ggacaagcgc atggccgcga
     2761 caggctctgc gagaaaggcc aagcggctcg gccttctcgc gctgggattc ctaggcgcgg
     2821 ccgcgctcgg cggctgccat gatgacgacg tcatcaccgg ctcgatcgcg ccggacgatt
     2881 atcacaatcg ccatccgatc gtgctgaccg acggtccgac cacgctcgat gttttcccga
     2941 ccggccagag cgcgctcgat gagcgttcgg cggccaatgt gcgggccttc gccgagcgct
     3001 acgcaaaata cggcgtcggc aggattgtgc ttctcgcgcc ggccggcggc gggccgaaga
     3061 ttcacgccgg catgaatgcg atccggcgcg aactggccgc cgcgggcttg aggggacgaa
     3121 ttgggatcgg ctcctatccc gtcgccgatc ccctgctcgc cgcgccgatc aagctgagtt
     3181 tcgtcgggtt gaaggcggag gtcccgccgc attgcggcca atggccggtc gatctcgcct
     3241 ccggcagctc gttggagacc tggaagaacg aaacctattg gaactatggc tgcgcgacgc
     3301 agtccatcct cgccgccgag gtcgacaatc cgcgcgattt cgtccaggcc ggcgcgctcg
     3361 gcccgtccga tgtccagatg aggctgcgcg ccatcaacgc cgtgcgcaac ggcacggatc
     3421 ccggcacggg atggaacgtc aaaaacacca gtatcgggca aatcggggga agctgaccga
     3481 tgagcgtcga tttcaacggc gtaggtccgg ccggcgcgac gcaagacgcg cccaatccgc
     3541 cgcaaatcaa tccggtgccg cgcatttcga ttcaggcctt ctgcgaaacg caggaggtcg
     3601 cggcggtgat ccaggccgcg atgagcgacc ggcggatgaa caaggcgcat gtcaagctgc
     3661 atatgggcgg cccggcggcg gccgtcgagg cctatcgcag cgcgccgacg ccgaacgtca
     3721 tcgtgctcga atcctcgcag gatcgcgacg cgctgaccgt cagcctcgac gaactggccg
     3781 agttctgcga cgccggcacc aaggtgatcg tctgcggcaa gatcaacgac atcgtccttt
     3841 atcgccatct gatcctgaaa ggcgtcagcg agtatttggt cgcgccgttc tcggtgctcg
     3901 atttcgtcca ggcgatctcc aatctctaca attcgcccgg cgccaatccg gtcggccggg
     3961 tgatcgccgt caccggcgcc aagggcggcg tcggcgcctc gaccatcgcg cacaatctcg
     4021 cctggtcggt ggcgaccggg ctcgaaacct cgacggtcat cgccgacatg gatctcggct
     4081 tcggcaccgc cggcctcgac tacaatcagg acccgccgca aggcatggcc gaggcggtgt
     4141 tcgcgccgga tcgcgtcgac tccaatctcg tcgagcggct tctgtcgaaa tgcgccgaca
     4201 atctcagcct gctggccgcg ccggcgacgc tcgagcggcc gtacgatttc ggcgagaccg
     4261 ccttcgacgc ggtgatcgat atcctgcgcg cctcgacgcc ctgcgtcgtg ctcgatgtcc
     4321 cgcatatctg gagcgcctgg accaagcgcg ctttggtcgg cgccgacgat gtgatcgtcg
     4381 tcgccgggcc cgatctcgcc aatttacgaa acgccaagaa tctcctcgac aatctgcgcg
     4441 gcgcgcggcc caatgaccgc agcccgaaat tgatcctgaa cggggtcggc gtggtgaaga
     4501 ggccggaaat cgcggtcgcg gatttcgcca agacggtgga ggtcgagccg gccgccgtca
     4561 ttccctacga cgccaagctt ttcggcgccg cggccaataa cggccagatg atcgcggaga
     4621 tcgaggccaa tggaaagacc gccgcgattt tcgccgagtt ggcgcgcgag ctgaacggaa
     4681 aactcgaatt gaagaaggcc aagcgcagcc tcttcgatcc tctcatctcg gcgcttgggc
     4741 gcaagaaggc gcgagcggcg taaaggagcg cgggttgatg gcggcctgga gatcggacga
     4801 ccatgttcgg taagcgcgcc agcggggcca atgccgccgc cgcgccgccc agcgcgccgg
     4861 cgcggccgct cgaatccgcg cccgcggagg ccgcgccgcg cgcctcggcg cccgagcccg
     4921 tccgcgccgc ggcggagccg cgccggtccg acagctatta tcaaaccaag agcatgattt
     4981 tcggcgcgct gatcgaggcg atcgatcttt ccgcgctggc caagctcgat ccggacaatg
     5041 cgcgcgagga aatccgcgac atcgtccacg agatcgtcac gatcaagaac atcgtcctgt
     5101 cgatcgccga gcaggaggac ctgctcgagg acatctgcaa cgacgttctc ggctatggcc
     5161 cgctcgagcc gctgctcgcc cgcgacgaca tcgccgacat catggtcaat gggccgaccc
     5221 gctgcttcat cgaagtcggc ggcaagattc agctcaccaa tgtgcgcttc cgcgacaatg
     5281 cgcaattgat gaacatctgc cagcgcatcg tcagccaggt cggccgccgc gtcgacgagg
     5341 cttcgccgat ctgcgacgca cgcctgccgg acggttcgcg cgtcaacgtc atcgcgccgc
     5401 cgctcgccat cgacggcgcc gcgctgacca tccgcaagtt caagaaggac aagctgacgc
     5461 tcgaccagtt gacgcgtttc ggctcgatct ctccggccgg cgcggaaatc ctcaagatca
     5521 tcggccgcgt ccgctgcaac gtcatcattt ccggcggcac cggctcgggc aagacgacgc
     5581 tgctcaattg cctgaccaat tatatcgacg tcgacgagcg ggtgatcacc tgcgaagacg
     5641 ccgccgaatt gcagctccag cagccgcatg tcgtgcgcct ggagacgcgg ccgcccaatc
     5701 tcgaaggcgc cggccagatc acgatgcgcg atctcgtcaa gaactgcctg cgcatgcggc
     5761 ccgagcgcat catcgtcggc gaagtgcgcg gcccggaagc tttcgatctg cttcaggcga
     5821 tgaataccgg ccatgacggc tcgatgggca cgctgcacgc caactcgccg cgcgaagcga
     5881 tgagccgcat cgaatcgatg atcacgatgg gcggctacgc gctgccgtcg cggacgatcc
     5941 gcgagatgat cgtctcctcg atcgacgtca tcatccaggc ggcgcgcctg cgcgacggct
     6001 cgcgccgcat cacccatatc accgaggtga tgggcatgga gggcgacgtc atcatcactc
     6061 aggacctatt cgtctacgag ctccttggcg aggatgcgca aggcaagttg aaagggcggc
     6121 accgctcgac cggcatcggc cggccgcgtt tttgggagcg cgcgcgctat ttcggcgaag
     6181 aggcgcgcct cgccgcggcg ctcgacgcct cggaagtcaa ggccgcgtaa ggagcagatc
     6241 gtgacgtcca atccgtttct gcttgccgtt ttcctcgtca tcgccctggg cggcggcgcg
     6301 ctcgtcctcg tctccggcga ttcgcgcgcc aagaagcgcc aggacgcgct gaagaagacg
     6361 acggtgaaga tgcgcgacgc cggcgccgac aagaacgcgc gccgcaagca ggtcgccgac
     6421 agcctcaaag agctcgaaca gcggaccggc cggaaaaaag cgactttgca ggcaaggctg
     6481 cagcaggccg gactcgcctt gacgccgcag caattctaca tcgtctcggc gctcggcgcg
     6541 gcttttgtcg cgctcgcgat ctttttcgtc actcgcagtc ttttcatcgc cgctccgtcg
     6601 gcgatcatcg gcggcttcgg cgcgccgaat ttcgtcctga actttctccg caagcggcgc
     6661 atcgccaaat tcgtcgccga gtttcccaac gccatcgaca tcatcgtgcg cggcgttaag
     6721 gccggcctgc cgatcggcga ctgcctgcgc gtcgtcgcca acgaatcggt cgagccggtt
     6781 cgcggcgagt tccgcgggat cgtcgaggcg caggcgctcg gtctttcgat cggcgaggcg
     6841 gtcgaccgcc tggcggagcg cgtgccggtc accgaagcca actttttcgc catcgtcatc
     6901 aacatccagg cgaaagccgg cggcaacctc tccgaggctc tgggcaatct ctcgcgcgtg
     6961 ctgcgcgacc gcaagaagat gcggggcaag atttcggcca tgtcgatgga ggccaaggcc
     7021 tccgccgcga tcatcggctg catcccgttc gtcgtgaccg ggctgctcta cctttccagt
     7081 ccaaaatacg tctcgttgct ctggaccacc agccacgggc gcatcatcgc ggcgatcggc
     7141 ctcgtctgga tggggatcgg cacgatgatg atgaagcgga tggtcagttt cgatttctaa
     7201 gcggacgctg aagagcctat gttcgatctc atcatcgaga aattgagcaa tccgcacttc
     7261 gtcgccggct tgctggtcgc ggtcggctgc gccgcgagcg tgctgacgct cgccatgccg
     7321 ctgctccaga ccgacaatct cggccgccgc atgaaagcgg tctccagcga gcgcgagcga
     7381 atccgccagc gcgagcgcga gcgcctgacg acgggccagc aaaaaccgtc gctgcgccag
     7441 gagcccaagc gcctgttcaa gcaggtcgtc gatcagctca atttgtcgac ctggctcggc
     7501 accgagaagg ccaagcagca attgtcgatg gccggctatc gcggcgccgg cgccgaatac
     7561 acgttcttgt tctttcgcct ggtgacgccg atcctgttct tttgcgtcac ggttttctac
     7621 gttttcgtcg tgctgcattg ggacaagccg ctggtcatga agatcggcgt cgccatcggc
     7681 gcggcctatc tcggcatcaa agggccggag atttttctct ccaacacgat cgccaagcgc
     7741 aagaaatcgc ttgaacgcgc ctatccgaac gcgctcgacc ttctgctcat ctgcgccgaa
     7801 tccggcatgt cgatcgagct cgccgtgcgc aaagtggcgc aggagatcgg cgtcgaatcg
     7861 gtgccgatgg cggaggaatt gacgctgctc gccgcggaaa tgtccttcct ctccgaccgg
     7921 cgccaggctt atgagaatct cggcgcccgc accggattgg aatcggtgcg cgcgctggtc
     7981 accgtgctca tccaggcgga gcgctacggc acgccgctcg gcacggcctt gcgcgtcctg
     8041 gcgcaagaaa gccgcgatca gcgcatgacc gccgccgaga aaaaggccgc cgccttgccg
     8101 ccgcaattga ccgtgccgat ggtgctgttc ttcctgccgg gcctctttgt cgccatcatg
     8161 acgccggcga ttatccagct taaacactgg acttgatcgg gccggacggc cgatccaccg
     8221 ctttgcgaat cgacaattgg cgcgcggcgc agcacattgc cgggcgatga ttcgcccctt
     8281 gttccgcgat cccgaagccg cgttccgcca gagcctcgcc aatccggccg atccgccggg
     8341 gcggggcggc ggcgcgccgc agagcatctc ggctcgggcg cgggccgcct tcgcgccgcg
     8401 ctatctcgaa gggctcaacc cggcgcagcg cgaggcggtg gaggcgcttg acgggccggt
     8461 cctggtcctg gccggcgccg gctccggcaa gacgcgcgtc ctcgccgtcc gcatcgccca
     8521 tctgatcgcc agcggccggg cgcgtccgca cgagattctc gccgtcacct tcaccaacaa
     8581 ggcggcgcgc gagatgcgcg agcggatcag cgtcctgacc ggcgtcgagg aaggcatgcc
     8641 gtggatgggc acgttccatt cgatcggcgc gaaaatcctg cgccgtcatg ccgaactggt
     8701 cggcctgcag ccgaatttca ccattctcga caccgacgat cagatccgcc tgctcaagca
     8761 gattctgcaa gccgaaaata tcgacgacaa acgatggccg gcgcgggcgc tcgccggcca
     8821 gatcgacgcg tggaagaatc gcggcctcga tcccgcccat gtctcggcgg ccgagtcggc
     8881 cgctttcgcc aatggccgcg gcgcggcgct ctatgccgcc tatcaggcgc ggatgaaaat
     8941 cctcaacgcc gccgatttcg gcgacctgct gctcgaaggc ctgcggctgt tccgcgagcg
     9001 cgaggacgtg ctgaaacggt accaggagcg cttccgctac attctggtcg acgaatacca
     9061 ggacaccaat gtcgtccaat atctctggct gcggctgctc gcccaggcgc ggcgcaatct
     9121 ctgctgcgtc ggcgacgacg accagtcgat ctatggctgg cgcggcgccg atgtcgacaa
     9181 cattttaagg ttcgaaaagg actttcccgg cgccaaggtc attcgcctcg agcgcaacta
     9241 ccgctcgacc ggccatattc tcgccgtcgc ctcgcatctc atcgcgcata ataagggacg
     9301 gctcggcaag acgttgttca ccgaggccga ggacggcgac aagccgaccg tcgcctgcgc
     9361 cgaggacagc caggaagagg cgcgcgtggt cggcgaggag atcgaggcgc tgcagcggcg
     9421 cggccagtcg ctcgacgaaa tcgcgatcct ggtccgcgcc tcgttccaga tgcgcgaatt
     9481 cgaggagcgg ttcatcacgc tcggcctgcc ttacaaggtg attggcgggc cgcgcttcta
     9541 cgagcgccag gaaatccgcg acgcgctggc ctatctgcgc tgcgtcgctc agcccgacga
     9601 cgatctcgcc ttcgagcgca tcttcaacgt gccgcggcgc ggcctcggcg aggcgacgct
     9661 gaccttgctg cacgattatg cgcgccgagc cggcctgtcg ctgatgcgcg cggcgcgggt
     9721 gatgatcgaa tcggaggagc tgaaggcgcg gccgcggcag attttgcgcg atctggtcca
     9781 gtccttcgac cgctggtcgg cgatggtcga gaagacgccg cacgaggagc tcgcccagac
     9841 cgtgctcgaa gagagcggtt atatcgacat gtggaaggcc gagcgcaccg ccgacgccgc
     9901 cggccggctc gaaaacctga aagagctgac gcgctcgatg gaggagtttc cttcgctcgc
     9961 cgccttcctc gaacatgtct cgctggttct cgacgccgag agcgacgagc agggcgagcg
    10021 cgtctcgatc atgaccctgc acgccgccaa gggtttggag ttcgacgtcg tctttctgcc
    10081 cggctgggag gaggggctct ttccgcatca gcgctcgctc gacgagaacg gccgcgccgg
    10141 cctcgaagag gagcgccggc tcgcctatgt cggcctgacc cgggcgcgcc ttcaggcgaa
    10201 aatctacttc gccgccaacc ggcgcattcg cggcctctgg cagtcgacgc agccgtcgcg
    10261 gttcctcgac gaattgccgg ccgaccatgt cgaggtcctc gaagcgggcg gcgcgccggc
    10321 ctatccggcc tcgcgcttcg atcgcgtcga gccgttcgcc tcgacatacg aatcgcccgg
    10381 ctggcggcgc gctcaggcgc gcgccaatgc gcccggcgcc gcggccgccc ggcgcgcgcc
    10441 ggtgacgatc gaaggccggc tgatggcgca atcggcggtc gaatcgcgct tcgccaaagg
    10501 ggcgcgggtc ttccacatca agttcggccc cggctcggtc gccgccgtcg acggcgccaa
    10561 gctgaccgtc gatttcgaca aagccggccg caaaatggtg ctggagagct tcgttcagcc
    10621 ggccgattga ggtccccgga gcgccgcccc gctgtagccg cggtcgccga ccgcggctac
    10681 aaacgtcgcg cgccgctttg cggataaagg agattgatgt ttcgcagcat tctctcggtc
    10741 ggcggcttca cgctgctctc gcggctgacc gggttcgcgc gcgatctcgc gctcggcgcc
    10801 ctgctcggcg ccgggccgat cgccgacgcg ttcgtcgtcg cttttcgcct gccgaattac
    10861 tttcgcgcga ttttcggcga gggcgccttc aacaccgcct atgtcccgtc ctatgcccgc
    10921 gtgctggaaa ccgaaggcgg cggcgaagcc aagcatttcg ccagccaggt tttcacgatc
    10981 ctcttcgtct cgcagatcgt cctactcgtc ttcgccttcg ccttcacgcc gcttctcgtc
    11041 cgggtgatcg cgccgggttt cgaagccgat ccggaaaagt tcgcgctcgc ggtccgcatg
    11101 acgcggatca cctttcccta tcttttcttc atcacgctgg cgacgctgca ttcgggaacg
    11161 ctcaatgcgc atggccgctt cgccgtcgcc gctttcgcgc cggtcctgct caatttgacc
    11221 atgttggcct tcctggccgt cgcctttcgt tttcccaatg ccggcgaggc ggcgagctgg
    11281 ggcgtggccg tttccggcgc gctgcaactc gccttgctca tgctggcggc gcggcggcgc
    11341 aatctgctcg aaggcttcgc gcggccgcgc ctcagcgccg acatccggca atttttcggc
    11401 gcgctggcgc ccgccgtgat cggctcggcc ggcgtccaga tcgccttgct cgccgattcg
    11461 atcatcgcct cgatgctgcc gaccggcgcg gtcgcctcca tctattatgc cgagcggctg
    11521 taccaattgc cgatcggcgt cgtcggcgtc gccgccggca cggtactgct gccgcagatg
    11581 agccggcgct tggcgagcgg cgataaggaa ggggcggccc gcgcgcagaa ccgcaccgcg
    11641 gcgctgacgc tcgctctgtc ggcgccgttc ttcgtcgcct tcattttggt tcccgagccg
    11701 atcatgcgcg gcgcctttct gcgcggcgcc ttcaccgcgg ccgacgcggc gcgctcggcc
    11761 gcggtgctcg ccgcctatgg aatcggcctg ctgccgatcg tcctgatccg ctcggcggtg
    11821 gcgagtttcc aatcgcgcgg cgacacgatg acgccgatgt tcgtctcgct cggcgccatc
    11881 gccgtcaatg tggcgctgaa attcgtcttc taccggtcgc tcggcgcagc gggcctggcc
    11941 ttggcgaccg ccattggcgc ctggctcaat cttgccgggc tgacgatgat cgcgctggcg
    12001 cgcggcctga tgcggcccga cactcatctc ggcgaagtcg cggcggcggc gtgctgcgcg
    12061 agcggcgtcc tggcgctcgc gctctcggtg agcctgcccg cggcggcgca gttcgccgcg
    12121 accgcgccgc attggcgcga cgagattctg ctcgtcatcc tcgccggaat cggcgccgcc
    12181 gcctatgtct tggcgttgct tgccgggctc ggcgcgctcg gcttcaggtt ccgccaggcg
    12241 cgcgctcaat cctcataatc atagccggga ccgtacgggc cgtaggccgg cgggcgcagg
    12301 acgacgcgcg gcgcgtagct tcccgccgcg gcctgcacga tgacgccctg atcggtcacc
    12361 acgacccatt cgcggccgcg cttctcgaag cgctcgacct tgccggcgcc gggcagcgaa
    12421 tcgccgggcg cgatttgatg catgccctga cggttctcga cgaccgccga gccgttgcgc
    12481 atctcgatca agtaccagcc gcggatcggc tcggtcggat gcggccgctc gatcgatccg
    12541 gtcacttcgt tggagacgcc cggcgaagcg gccgccgcct tctgcggcgg cagcggcgcg
    12601 gcggcggatg cggtttgcgc cgcgactttc ttctcgacct tgtcgatgcg cgccgaaagg
    12661 tccgcctttt ccagcttgtc gacgcgcgcg gcgaggtcgg atttctcaag tttgtcgagc
    12721 cgcgcggcga cgtcggtctt ttccagccgg tcgaggcgct gggcgagatc ggcgctgcgc
    12781 gtttgcgatt cagcgccgcg ggcggcgacc tcgcgctcga gccggtcgcc gagcttgtcg
    12841 agacgggcct ggtgctcgcg ctcgagccgg tcgaggcgcg cgttcaactg ggcgatggtc
    12901 gcggaaagat cgcgcgacgt ggcgaggccg ccgcgcgctt cgctgacggc tttgcggatt
    12961 tcggcggttt cgtcgcgggg cctcgccgtc tcgaccgcgg cgatccgcgc cgacatcgcg
    13021 ctcaccgcct gagcgaggcg ctgcgactcg cgctccgatt tcgccgcccg ttcgagcgtc
    13081 gcgccgcctg cgacgatggc gaccaaagcg aggcaagcgg ccgcggcggc ggtctggaaa
    13141 cgccggtcgg cggcgaagcg gccgagcgcg ccgagggcgg agccttgccg gcgcggggcg
    13201 acgaacggaa tcagcgtcgc ccgcgccggc tcgaccggcg gcgcttctcg cgccggcggc
    13261 tctgcggacg cacccgcatt ggcgcgaatc tcgatcggct cgggacgggc ttcggcgccc
    13321 gcctgacccg ccgcagcgga cggcgcctcg ctcgcggcgc tttcggccag cggcgctttg
    13381 tcggcggcca tgtcgggcgc ggcgatctgg gaattgtcgg catcgttgaa atccggggcg
    13441 tcctgcatgg cggcggtccg ttggttaacc aaatgcttca attgtgctt
//
LOCUS       DATGJW010000387         5338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_280043, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000387 DATGJW010000000
VERSION     DATGJW010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5338
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_280043"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VKU03_11700"
     CDS             <1..235
                     /locus_tag="VKU03_11700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ71973.1"
                     /translation="SGSSGMTMCWRSQQLNVKGGPATRPFRQKMIFTEADGRREEQTR
                     RPVRNAAVSCVTDCLASAPSLDLLRLHGHGRAH"
     gene            288..614
                     /locus_tag="VKU03_11705"
     CDS             288..614
                     /locus_tag="VKU03_11705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011650854.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NIPSNAP family protein"
                     /protein_id="HLJ71974.1"
                     /translation="MIYELRVYRVAPNQMPKLLARFRDHTLAIWERHGIRQLGFWTTL
                     VGDSNHELTYILQWESWEDRERRWNAFLKDPAWLRARDESERDGPLIANIANQMLAPT
                     DFSALK"
     gene            complement(668..2497)
                     /gene="recQ"
                     /locus_tag="VKU03_11710"
     CDS             complement(668..2497)
                     /gene="recQ"
                     /locus_tag="VKU03_11710"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614570.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA helicase RecQ"
                     /protein_id="HLJ71975.1"
                     /translation="MTAPAALLEKARAALHGVYGFESFRPGQEEIVSALLAGEDVIAI
                     MPTGGGKSLLYQLPALARDGLTLVASPLIALMRDQVAQLRELGVAAAALNSASEPEER
                     RQVAVALRDGSLRLLYVAPERLLRDDMIAFLAKQKIDLFAIDEAHCVSQWGHDFRPEY
                     MRLKEVAEALGGVQTIAVTATADAPTRAEIAKKLFLRRPRQFVRSFDRPNLFLAMTPK
                     ANATRQLMDRLDGHRDESGIIYCASRRRTEDLASQLSAQGSRALPYHAGLDQDVRSKN
                     QDIFLKEDGVVICATIAFGMGIDKPDVRFVFHADLPSSIEAYYQEIGRAGRDGLPADT
                     FTLYGAGDIALRRRQIEESNAPDERKRVEMKKLDDLVALCETARCRRQVLLGFFGEEV
                     EPCGHCDVCKGAVRLVDGTIDAQKAMSAILRTQGRFFFGHLANILSGKSTDAVLLHGH
                     DKLKTFGAGKDRTPAEWRGVLRQLLSAKLLLFDPADRDRLGISDEGMKVLRGQAAFSL
                     REDIGGGRKRRGRLARETEPASESAAEFRPATLAALKALRTDLARRQDVPAYVIFPDR
                     TLMEMAARRPKTIAELATIHGVGTAKLKRYGEMFLAAIQSVDV"
     gene            complement(2494..3558)
                     /gene="epmA"
                     /locus_tag="VKU03_11715"
     CDS             complement(2494..3558)
                     /gene="epmA"
                     /locus_tag="VKU03_11715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004824 - lysine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006430 - lysyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EF-P lysine aminoacylase EpmA"
                     /protein_id="HLJ71976.1"
                     /translation="MTAPSPYWSPDRHLDRRPRLLVRSRILAALRRHFAAEDFVEVEP
                     AILQASPGNETHLHGFATEMIAPGGARAAAFLHTSPEFAMKKLLAAGETRIFALARVF
                     RNRERGALHAPEFTMLEWYRADAPYQQLMEDCAALLATAADAAGAARLAFRGREADPR
                     AAPERLAVREAFLRHAGVDLFATLSADGAERDRDGLASQTLAIGLRVAPDDDWSDLFS
                     RILSEKVEPMLGLGRPTILCDYPISEAALARAHPDDPRLAERFELYACGVEVANAFGE
                     LTDPAEQRRRFEADMADRQRLYGEAYPIDEDFLAALAAMPAASGAALGFDRLVMLACG
                     AERIDDVQWTPVFDPFAERP"
     gene            3734..4303
                     /gene="efp"
                     /locus_tag="VKU03_11720"
     CDS             3734..4303
                     /gene="efp"
                     /locus_tag="VKU03_11720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor P"
                     /protein_id="HLJ71977.1"
                     /translation="MRVIASSIRKGNIIEREDGQLYVVLSAESFHPGKGTPTTQIDMR
                     RISDGVKVSDRFKTTEQVERAFVEDKEFSFLYQDGDGYHFMNSDTYDQVQVPEDVIGE
                     QAQWLQEGMKVMLSMFNGVAVAIQLPQRVTLEVTETEPAMKGQTASSSYKPAKLSNGA
                     RAMVPPHITTGTRVVIQTEDGSYIERAKD"
     gene            4432..>5338
                     /gene="gnd"
                     /locus_tag="VKU03_11725"
     CDS             4432..>5338
                     /gene="gnd"
                     /locus_tag="VKU03_11725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017483256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004616 - phosphogluconate dehydrogenase
                     (decarboxylating) activity [Evidence IEA];
                     GO_process: GO:0006098 - pentose-phosphate shunt [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decarboxylating 6-phosphogluconate
                     dehydrogenase"
                     /protein_id="HLJ71978.1"
                     /translation="MQLGMIGLGRMGANIVRRLMRNGHSCVVYDRDPKPGQALALEGA
                     KAVASLEDMVKALAPPRAIWIMLPAGAPTEDTIAALAALAARGDVIIDGGNSFWQDDI
                     RRGAELAKKGLHYLDVGTSGGVWGLERGYCMMIGGEAEVVRRLDPIFKTLAPGRGTIA
                     PTPNRDGRDARVEEGYLHAGPCGAGHFVKMVHNGVEYGMMQAYAEGFDILRNADSQSL
                     KAEHRFSLNLADIAEVWRRGSVVTSWLLDLTAAALAHEGELASYSGYVEDSGEGRWTL
                     MAAIEEAVPAEVLSAALYTRFRSRQE"
BASE COUNT          983 a         1702 c         1767 g          886 t
ORIGIN      
        1 ttcgggaagt tccggcatga cgatgtgttg gcgcagccaa cagctgaacg tcaagggcgg
       61 accggcaact aggccgtttc gtcaaaagat gattttcact gaagccgatg gtcggcgcga
      121 agagcaaaca aggcgccctg tccgcaatgc agccgtgtcc tgcgtgactg attgcctcgc
      181 ttcggcgccg tcgctcgatt tgctgcgctt gcatggacat gggcgcgctc attgacaggc
      241 cgaaggaggc gcgcctaaga cagaatattg cgctgggagg cataccgatg atttatgaat
      301 tgcgggtcta tcgcgttgcg ccaaaccaga tgccgaaatt gctggctcgg ttcagagacc
      361 ataccctcgc aatttgggag aggcacggca tccgccagct tggcttttgg acgacgctcg
      421 tcggcgattc caatcacgaa ttgacctaca tccttcaatg ggaatcgtgg gaggaccgcg
      481 agaggcgatg gaacgccttc ctcaaggatc ccgcctggct tcgcgcgcgc gacgaaagcg
      541 agcgcgacgg accgctcatt gcgaacatcg ccaaccagat gctcgcgccg acggacttct
      601 ccgccctgaa ataatgccct tccgcgttgg atggcgcggg cgccgctccg ccatccaacg
      661 aactccttta aacgtcgacg ctttgaatcg cggcgaggaa catctcgcca tatcgcttca
      721 actttgcggt cccgacgcca tggatcgtgg caagttcggc gatcgtcttt ggccggcgcg
      781 ccgccatctc catcaaggtc cgatccggaa agatcacata agccggcacg tcctgccggc
      841 gggcgaggtc cgtccgcagc gccttcaacg cggccagcgt cgccgggcgg aattccgccg
      901 cggattcgga cgcaggctcc gtttcgcgcg caagtctgcc gcgccgtttc ctgccgccgc
      961 cgatatcctc gcgcagcgag aaggccgcct gaccgcgcag caccttcatg ccctcgtcgc
     1021 ttattccgag acggtcgcgg tcggccgggt cgaagagaag cagcttcgcc gacagcaatt
     1081 gcctcaagac gccgcgccat tccgccggcg tccggtcttt tccggcgccg aacgtcttga
     1141 gtttgtcatg gccgtgcaga agcaccgcgt cagtcgactt gcccgagaga atattggcga
     1201 gatggccgaa gaagaagcgc ccttgcgtgc gcaggatcgc cgacatcgct ttttgcgcgt
     1261 cgatcgtccc atcgacgagc cgcacggcgc ctttgcagac atcgcaatgg ccgcatggct
     1321 cgacctcttc gccgaaaaag ccgagcagga cttggcggcg gcagcgcgcg gtctcgcaaa
     1381 gcgcgacgag gtcgtcgagc ttcttcatct cgacccgctt gcgctcgtcg ggggcgttgc
     1441 tctcctcgat ctgccgccgg cgcaaagcaa tgtcgcccgc gccgtacagc gtgaacgtgt
     1501 cggccggcag accgtcgcga ccggcgcggc cgatctcctg gtaataggcc tcgatcgagg
     1561 acggcagatc ggcgtgaaag acgaagcgaa cgtcgggctt gtcgatgccc atgccgaaag
     1621 cgatcgtcgc gcagatgacg acgccgtcct ctttcaggaa gatgtcctgg ttcttgcttc
     1681 gaacgtcctg atcgaggccg gcatggtacg gcaaggcgcg cgagccctga gccgagagct
     1741 gcgaggccag atcctcagta cgccgccgtg acgcgcaata gatgatcccg ctctcgtctc
     1801 tatgcccatc gagcctgtcc ataagctgcc gcgtcgcatt ggcctttggc gtcatcgcca
     1861 gaaaaagatt tgggcgatca aaagaacgaa cgaactgacg cggacggcgc aggaacagtt
     1921 tcttggcgat ctcggctctt gtcggcgcgt cggccgtcgc ggtcaccgcg atcgtctgca
     1981 cgccgcccag cgcctcggcg acttctttga ggcgcatata ttcgggccgg aagtcgtgcc
     2041 cccattgcga gacgcaatgg gcctcgtcga tggcgaagag gtcgatcttc tgcttggcga
     2101 ggaaggcgat catgtcgtcg cgcagcagcc gctcgggcgc gacatagagc aggcgcagcg
     2161 aaccgtcgcg caaggccacg gcgacttgcc ggcgctcctc cggctcggaa gccgaattca
     2221 aggccgcggc ggcgacgccg agctcgcgca attgcgccac ctggtcgcgc atcagcgcga
     2281 tcaagggcga agcgaccagc gtcaagccgt cgcgcgccaa ggccggcaat tggtacagca
     2341 gcgacttgcc gccgccggtc ggcatgatcg cgatgacgtc ttcgccggca agcagggcgg
     2401 aaacgatctc ctcctggccg ggccggaacg attcgaagcc gtagacgccg tgcagcgcgg
     2461 cgcgagcctt ttcgagaagc gcggccggcg ccgtcatggc cgctccgcga acggatcgaa
     2521 gaccggcgtc cactgcacgt cgtcgatccg ctcggcgccg caagcgagca tgacgaggcg
     2581 gtcgaagcca agggccgcgc cgctggcggc cggcatggcg gcgagcgcgg cgaggaaatc
     2641 ctcgtcgatc ggataggctt cgccatagag ccgttggcgg tcggccatat ccgcttcaaa
     2701 tcggcgacgc tgctcggccg gatcggtcaa ctcgccgaac gcattggcga cctcgacgcc
     2761 gcaggcgtag agctcgaagc gctcggcgag gcgtgggtcg tccggatggg cgcgggcgag
     2821 cgccgcctcg gagatcggat aatcgcacag gatcgtcggc cggccgaggc cgagcatcgg
     2881 ctcgaccttt tcgcttaaaa tccggctgaa cagatcggac caatcgtcat ccggcgcgac
     2941 gcgcagcccg atcgccagcg tttggctcgc caagccgtcg cgatcacgct ccgcgccatc
     3001 ggcggagagg gtggcgaaga gatcgacgcc cgcatggcgc aaaaacgcct cccggacggc
     3061 gaggcgctcg ggcgcggcgc gcggatcggc ctcgcggccg cgaaaggcga ggcgggcggc
     3121 gccggccgcg tcggctgcgg tcgcgaggag ggccgcgcaa tcctccatca attgttgata
     3181 gggcgcgtcc gctcgatacc attcgagcat ggtgaattcc ggcgcatgaa gcgcgccgcg
     3241 ctcgcgattg cggaagaccc gcgccagggc gaagatgcgc gtctcgccgg cggcgagcag
     3301 cttcttcatc gcgaattccg gcgacgtatg caggaaggcg gccgcccgcg cgccgcccgg
     3361 cgcgatcatt tccgtcgcaa aaccatggag atgggtttcg ttgccgggcg aggcctggag
     3421 gatcgccggc tcgacctcga cgaagtcctc cgccgcgaaa tgacggcgca aagcggcgag
     3481 gatccgcgag cggacgagaa ggcggggccg gcggtcgaga tggcgatcgg gagaccagta
     3541 aggcgaaggc gcggtcaagg ctcgtccagg cggattggag gccggcgggc cggaacgatc
     3601 gcgtctggcc ttcggcggca aaatcggtat gttgccggcc gccgaagcgc aaggccggcg
     3661 cgcaatcgtc cgccgcatca gcgctttcac tggatgtcgg gccgcaacgg cccatctcga
     3721 aggatttgaa aacgtgagag tcatcgcaag ctcgatccgc aaaggcaaca ttatcgagcg
     3781 ggaagacggc cagctttatg tcgttctgtc ggccgagagc ttccatccgg gcaagggcac
     3841 gccgacgacc cagatcgata tgcgccggat ctccgacggg gtgaaggtct cggaccgctt
     3901 caagaccacg gaacaggtcg agcgcgcctt cgttgaggac aaggagttca gtttcctcta
     3961 ccaggacggc gacggctatc acttcatgaa ttccgatact tacgaccagg tccaggtgcc
     4021 ggaagacgta atcggcgagc aggcgcaatg gctgcaggaa ggcatgaagg tcatgctgtc
     4081 gatgttcaac ggcgtcgccg tcgccattca attgccgcag cgcgtgacct tggaagtgac
     4141 cgagaccgag ccggcgatga agggtcagac cgcctcttca tcatacaagc cggcgaaatt
     4201 gtcgaacggc gcgcgcgcca tggtgccgcc acatatcacg accggaacgc gcgtcgtcat
     4261 ccagaccgaa gacggctcgt atatcgagcg cgccaaggat taaaggcgcg atgaaaccca
     4321 cccgtcattg cgagcgaagc gaagcaatcc attcataggg ctgagccccg cgagtggatt
     4381 gcttcgtcgc ttcgctcctc gcaatgacga cctgattttg cggaggcggc gatgcagctc
     4441 ggaatgatcg gcttaggcag gatgggcgcc aatatcgtgc gccggctgat gcgcaacggc
     4501 cattcctgcg tcgtctacga ccgcgacccg aagcctggcc aagcgctcgc tttggaaggc
     4561 gcgaaagccg tcgcctcgct cgaagacatg gtcaaggcgc tggcgccgcc gcgcgcgatc
     4621 tggatcatgc tgccggccgg cgcgccgacc gaagacacga tcgcggcgct cgccgcgctc
     4681 gccgcgcgcg gcgacgtgat catcgatggc ggcaattcgt tctggcagga cgacatccgc
     4741 cgcggcgccg agctcgccaa gaagggcctg cactatctcg atgtcggcac gagcggcggc
     4801 gtctggggcc tggagcgcgg ctattgcatg atgatcggcg gcgaggcgga ggtcgtgcgc
     4861 cggctcgatc cgatcttcaa gacgctggcg ccgggacgcg ggacgatcgc gccgacgccc
     4921 aatcgcgacg gccgcgatgc gcgtgtcgaa gagggctatc ttcacgccgg accgtgcggc
     4981 gccgggcatt tcgtcaagat ggtccataac ggcgtcgaat acggcatgat gcaggcctat
     5041 gccgagggct tcgacatttt gcgcaatgcc gacagccaat cgctgaaggc ggagcatcgc
     5101 ttctcgctga acttggccga catcgccgaa gtctggcgcc gcggcagcgt cgtcacctcg
     5161 tggctgctgg atctgaccgc ggcggcgctc gcccatgaag gcgagctcgc ctcctattcc
     5221 ggctatgtcg aggattcggg cgaaggccgc tggacgctga tggcggcgat cgaagaggcc
     5281 gtgcccgcgg aagtcctttc cgccgcgctc tatacgcgct tccgctcccg ccaggagc
//
LOCUS       DATGJW010000388         5121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_73804, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000388 DATGJW010000000
VERSION     DATGJW010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5121
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73804"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..607
                     /locus_tag="VKU03_11730"
     CDS             <1..607
                     /locus_tag="VKU03_11730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020047383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ71979.1"
                     /translation="AQIAMFKKEKCEIFNSFPIPPDFAAFWRQAAQQGYSKMVKICQT
                     AKTGLFPTTVEAFGALGYNFSSAAYWHRAFPYKSSLTGISSRELADGYEKTSGKQWTQ
                     QLGASLALIDAGVEALKQSGAPKDKAKVAAAMAKLNIMTINGKVDFTSGPVPNVSPGP
                     IIGAQWVKAKPGSKFKLDYPTTEHATDPNVPIEAKLVPYNS"
     gene            689..1441
                     /locus_tag="VKU03_11735"
     CDS             689..1441
                     /locus_tag="VKU03_11735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012490352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71980.1"
                     /translation="MNETSAKPSSREILAARGIVKRFNALTVLDGVDFAMRANEAVGV
                     VGPNGAGKTTLLSVLSGALPPSAGRVSFEGVDVTSLPAAERCRLGLARTHQIPRPFGG
                     LTVFENVFVAAANRPRFTRAEAYDLCIDALRLCGMLSLSNRRAEALGLLDRKRLELAR
                     ALAAKPVALLLDEIGGGLTDAETSELVDIIKELKRRGVALIWIEHIVHILLQVADRLV
                     CMNAGRILAEGEPRKVMADPEVIEAYLGGAAA"
     gene            1438..2169
                     /locus_tag="VKU03_11740"
     CDS             1438..2169
                     /locus_tag="VKU03_11740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856756.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ71981.1"
                     /translation="MSALQVEKLSARHGLLHAVRDVSFAVEKGQILALIGANGAGKTT
                     LLRAIAGAHPLHSGRVLLDGVDLGRARAHERTKRGVALVPEGRRLFAGMTVEDNLRLA
                     GGVGRTGPWSIESVLETFPNLQPRRKALAGSLSGGEQQAAAIGRALMTNPEVLLLDEV
                     SLGLSPLAVERVYASLQTLMRSGVTVILVEQDLKRALSVADQVICMLEGRFVAEGKAS
                     EMTREEVTAAYFGLHRAAPQVGTAP"
     gene            2166..3065
                     /locus_tag="VKU03_11745"
     CDS             2166..3065
                     /locus_tag="VKU03_11745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010914661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ71982.1"
                     /translation="MILVNQIIQGVLLGGYYALIACGLSFMFGVMRIINLAHGALAIL
                     AAFALFVLADSYGLSPFVALLLVLPAMAAIGWLLQRGVLERSQRGGVLTPILSTFGLA
                     IVIENLLFQRFGADTRSLAPYVDTLSYDSWSVTDDISIGKLAVLILLAAIVELGGLQL
                     LLAKTQIGRAIRATAEDADTASLVGVDARFVKAAATAIAMVTVGIAGAALALRATFDP
                     YAGPMQLIFAFEATVIGGAGSLWGTLVGGVVLGVAQSLGAQVSPQGFFLAGHAVFLAI
                     ILIRVFAGETIRAKLPRFRRRPA"
     gene            3062..4099
                     /locus_tag="VKU03_11750"
     CDS             3062..4099
                     /locus_tag="VKU03_11750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ71983.1"
                     /translation="MSARPIVQRWTRASFASLLVVAAIAILLAAGPFLFNANAIDKLT
                     TLFIYVMLAVTWNALAGYGGLVSVGQQAFFGLGAYFAVRLAGWGFPVFPALIVAGLIV
                     GALALPVSVLMLRLREGEFAIGMWVVAEVAHLLVNLDKLVRGETGTSLIALDAYQATL
                     RHAVVYWLALASTLAVAGALFWLLRSRTGASIQAIRDNEEAAASLGVRVMATKRVLFV
                     LAALGAGLAGALWLASFLTFQPKTYFSVQWSAYMIFMVLVGGLGTFEGPILGAVLFFL
                     IEALFGAAGVWYLVGLGATALVFSLFLPRGLWGLLQDRFALQLLPVGYRLIMKGGPAI
                     RIDEKTYSTNS"
     gene            4149..4934
                     /locus_tag="VKU03_11755"
     CDS             4149..4934
                     /locus_tag="VKU03_11755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003550970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="coniferyl-alcohol dehydrogenase"
                     /protein_id="HLJ71984.1"
                     /translation="MLLGKTVVVTGVSSGIGARTAELAGQLGADVIGVDIKEPSAPLG
                     AFVKADLSSPQSIAELADTLPQRIDALCNVAGLSGLVGAVKTLAVNFYGLRAISEALA
                     PRIREGGAIVNVASIAGYGWRANLERAKAMIAPSGFPDIARLLAAYDVPDAEGYPLSK
                     ELLLLWTQRAAHQPLFKDRGLRVNAVSPGPVTTPILKEFRAVFGDPRVDSDIARVGRA
                     GAPPDIAPVILFLCSDGARWINGANIPADGGLEASINQEVLGF"
BASE COUNT          761 a         1737 c         1657 g          966 t
ORIGIN      
        1 ggcgcagatc gcgatgttca agaaagagaa atgcgagatt ttcaactcct tcccgatccc
       61 gccggatttc gccgccttct ggcgccaggc cgcccagcag ggctattcga agatggtaaa
      121 aatctgccag acggccaaaa ccggactctt cccgaccacg gtcgaagcct tcggcgcgct
      181 cggctacaat ttctcgagcg ccgcctattg gcaccgcgcc ttcccgtaca agtcgtcgct
      241 gaccggcata tcgagccggg agctcgccga cggttacgag aagacctcgg gcaagcaatg
      301 gacgcagcag cttggcgcgt cgctggcgtt gatcgacgcc ggcgtcgaag cgctcaaaca
      361 aagcggcgcc ccgaaggaca aggcgaaagt cgccgcggcg atggccaagc tgaacatcat
      421 gacgatcaac ggcaaggtcg atttcacctc cggccccgtg cccaatgtct cgcccggccc
      481 gatcatcggc gcgcaatggg tgaaggcgaa gccggggtcg aagttcaagc tcgactaccc
      541 gacgaccgag cacgccaccg atccgaacgt gccgatcgag gcgaagcttg ttccgtacaa
      601 ttcctgagcc tcgcttcgtc aagaccgcgc cctctcccgc cggcgtgaga gggcgcgcgt
      661 cgcagccttt gcgatggcgc gcggcccgat gaacgagacg agcgccaaac cgtcgtcgcg
      721 cgaaatcctc gccgccagag gaatcgtcaa gcgcttcaat gcgctgaccg ttcttgacgg
      781 cgtcgacttc gcgatgcgcg ccaacgaggc ggtcggcgtc gtggggccga acggcgccgg
      841 caagacgacg ctgctctccg tactttccgg cgcgctgccg ccaagcgccg gccgggtcag
      901 tttcgaaggc gtcgatgtga cgagccttcc cgcggccgag cgctgccggc tcggcctggc
      961 gcgcacccat cagatccccc gtcccttcgg cggcctgacc gtcttcgaga acgtcttcgt
     1021 cgcggcggcc aatcggccgc gattcacccg cgccgaggcc tatgatctct gcatcgacgc
     1081 tttgcggctt tgcggcatgc tgtctctctc caatcgtcgc gccgaggcgc tcggcctgct
     1141 cgaccgcaaa agactggagc tcgcccgcgc tctcgccgcc aagccggtcg ccctgctgct
     1201 cgatgagatc ggcggcggac tgaccgacgc ggaaacgagc gaactcgtcg acatcatcaa
     1261 ggagctgaag cgccgaggcg tcgcgctgat ctggatcgag cacatcgtcc acatcctgtt
     1321 gcaggtcgcc gaccgcctcg tctgcatgaa cgctggccgt atcctcgccg agggcgagcc
     1381 gcgcaaggtc atggccgatc ccgaggtgat cgaagcttat cttggcggcg cggcggcatg
     1441 agcgcgctgc aagttgaaaa gctgagcgcc cggcacggcc tcttgcacgc ggtgcgcgac
     1501 gtctccttcg ctgtcgagaa gggccagatt ctcgcgctta tcggcgccaa tggcgcgggc
     1561 aagacaacgc tgctgcgcgc catagccggc gcgcatccgc ttcattccgg ccgcgtgctg
     1621 ctcgacggcg tcgatctcgg ccgggcgcgc gcgcacgagc ggacgaagcg cggcgtcgcg
     1681 ctcgtacccg aagggcgccg gctcttcgcc ggcatgaccg tcgaggacaa tctgaggctc
     1741 gccggcggcg tcggccgtac cggcccttgg tcgatcgaat ccgtcctgga gacatttccg
     1801 aacttgcagc cgcgccgcaa ggcgctggcc ggcagcctct cgggaggcga acagcaggcg
     1861 gcggcgatcg gccgggccct gatgaccaat cctgaggttc tgcttctcga cgaagtctcg
     1921 ctcggcctgt cgccgctcgc cgtcgaacgc gtctatgctt cgcttcaaac actgatgcgc
     1981 tcgggcgtca cggtgatcct cgtcgagcag gatttaaagc gcgcgctgag cgtcgccgac
     2041 caggtgatct gcatgctcga agggcgcttc gtcgccgagg gcaaagcctc ggaaatgaca
     2101 cgggaggagg tcacggccgc ttatttcggc ttgcatcggg ccgcgcccca agttgggacg
     2161 gcgccatgat cctcgtcaat cagatcatcc agggcgtgtt gcttggcggc tattatgcgc
     2221 tgatcgcctg cggcctttct ttcatgttcg gcgtgatgcg gatcatcaat ctcgcccatg
     2281 gcgcgctggc gatcctggcg gcctttgcgc ttttcgtcct tgccgattcc tatggcctgt
     2341 cgcccttcgt cgctctgctc ctcgttctgc cggcgatggc ggcgatcggc tggctcttgc
     2401 agcggggcgt gctcgaacgc agccagcgcg gcggcgtttt gaccccgatt ctttcgacat
     2461 tcggcctcgc catcgtcatc gagaatctcc tgttccagcg cttcggcgcc gacacgcgct
     2521 cgctcgcgcc ctatgtcgat acgctctcct acgattcgtg gtcggtcacc gacgacatca
     2581 gcatcggcaa gcttgccgtc ctgatcctgc tcgcggccat cgtcgaactc ggcggcttgc
     2641 agcttctgct cgccaagacg cagatcggcc gcgccatccg ggcgaccgcg gaggacgccg
     2701 acacggcgag cctcgtcggc gtcgatgcgc gattcgtcaa agcggcggcg accgccatcg
     2761 cgatggtcac ggtcggaatc gccggcgcgg cgctcgcctt gcgcgcgact ttcgatccct
     2821 atgccggccc gatgcagttg attttcgcct tcgaagcgac cgtaatcggc ggcgccggct
     2881 cgctttgggg gacgcttgtc ggcggcgtcg ttcttggcgt cgcgcaaagc cttggcgcgc
     2941 aagtgagccc gcagggattc ttcctcgccg gccacgcggt gttcctggcg attatcctca
     3001 tccgcgtttt cgccggcgag acgataagag cgaagctgcc gcgctttcgg aggcggccgg
     3061 catgagcgcg cggccgatcg tccagcgctg gacccgcgcc tcgtttgcga gtctccttgt
     3121 tgtcgcggcg atcgcgatcc tcctcgccgc cggcccgttc ctcttcaacg cgaacgccat
     3181 cgacaagctg acgacgcttt tcatctacgt gatgcttgcc gtcacatgga acgcgctcgc
     3241 cggctatggc gggctcgttt cggtcggcca gcaggccttt ttcggccttg gcgcctattt
     3301 cgcggtgcgc ctcgccggct ggggttttcc ggtctttccg gccctcatcg tcgccggact
     3361 cattgtcggc gcgctggcct tgccggtctc ggtcctgatg ctgcggctgc gcgagggcga
     3421 attcgccatc ggcatgtggg tcgtcgccga agtcgcccat ctcctcgtca atctcgacaa
     3481 attggtgcgc ggcgagaccg gcacctcgct gatcgcgctc gacgcgtatc aagcgacgct
     3541 gcgccatgcg gtcgtctatt ggctggcgct cgcttcgacg ctcgccgtcg ccggcgcgct
     3601 gttctggctg ctgcgcagcc ggaccggcgc ctcgatccaa gcgatccgcg acaatgagga
     3661 ggcggcggcc tcgctcgggg tgcgcgtcat ggcgacgaag cgcgtcctct tcgtcctcgc
     3721 cgcgctcggc gcagggctcg ccggcgcgct gtggctggcg agcttcctga cgttccagcc
     3781 gaagacctat ttcagcgtgc aatggtccgc ctacatgatt ttcatggtcc tggtcggcgg
     3841 cctcggaact ttcgaaggcc cgatcctggg cgcggttctg ttctttctga tcgaagcctt
     3901 gttcggcgcg gccggcgtct ggtacctcgt cggcctcggc gcgacggcgc tggttttctc
     3961 actgttcctg ccgcgcggcc tttgggggct cctgcaggat cggttcgcgc tgcaattgct
     4021 gccggtcgga taccggctga tcatgaaggg cggaccggcg atccggatcg atgagaagac
     4081 ctactcgaca aattcttgat caagacttgg cggcctgaag cgggcgaatg cgatatggga
     4141 ggacctggat gctcctcggt aaaaccgtcg ttgtgaccgg cgtttcgtcc ggcattggcg
     4201 cgcggacggc ggagttggcg ggacagcttg gcgcggacgt gatcggcgtc gacatcaagg
     4261 agccctccgc gccgctcggc gctttcgtca aagccgatct ctcctcgccg caatcgatcg
     4321 cggagctcgc cgatacgctg ccgcaacgca tcgacgcgct ctgcaacgtc gcgggccttt
     4381 ccggccttgt tggcgcggtc aagacgcttg ccgtcaattt ctatggcttg cgcgcgatca
     4441 gcgaggcgct ggcgccgcgc atccgggagg gcggcgcgat cgtcaatgtc gcctccatcg
     4501 ccggctatgg ttggcgcgcc aatctcgagc gcgccaaggc gatgatcgcg ccgagcggct
     4561 ttcccgacat agccaggctg ctcgccgcct atgacgtgcc cgacgccgag ggctatcccc
     4621 tgtcgaagga gttgcttctg ctctggacgc agcgggccgc tcaccagccg ctgttcaaag
     4681 atcgcggcct gcgcgtcaac gccgtcagcc ccggcccggt gacgacgccg atcctgaaag
     4741 aattccgcgc cgtcttcggc gatccgcgcg tcgacagcga catcgcccgg gtcggccgcg
     4801 ccggcgcgcc gccagacatc gcgccggtca ttctcttcct ctgctcggac ggcgcgcggt
     4861 ggatcaacgg cgccaacatc ccggcggacg gcggcctgga ggcgtcgatc aatcaggaag
     4921 ttttggggtt ttagcgcgac ctgattgatt ggggatcgcg agcgcggcaa tcgtcgtcac
     4981 gcgccgcgaa accgcgggct acggcgccgc tttcagcgcg aagagttcga ccgggctctt
     5041 cttgccgcgc aacgggattg gtcccagcgg gcgcgcgccg aagcctggcg ggagggccgt
     5101 caggctgcgc atcagcgccc c
//
LOCUS       DATGJW010000389         4020 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_903803, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000389 DATGJW010000000
VERSION     DATGJW010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4020)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4020)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4020
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_903803"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..428)
                     /locus_tag="VKU03_11760"
     CDS             complement(<1..428)
                     /locus_tag="VKU03_11760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973045.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ71985.1"
                     /translation="MKLGFAMALLSATLLAGSAYAAEKRWDGADDLPVSPLACSANEA
                     SPAPAAKPYDGGQPTNPPNKAGKPISLVDVPKLIGIGYFNATSKGMQEAAKELGNVTV
                     KTDGPTEAKIDEQIKFIDNYITSGVDGILFAANDPVAIAPV"
     gene            complement(479..1471)
                     /locus_tag="VKU03_11765"
     CDS             complement(479..1471)
                     /locus_tag="VKU03_11765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973044.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71986.1"
                     /translation="MSAMSEIPLPRAAKRAAPWWLIRHETMLAAILVVALIILGVLNN
                     RFLTIDNLLNQGRLTTEVGLIALPMTFIIVTGGIDLSVGSTVGLCAILLGYSWKNFDF
                     PLPLAIVFCLLVGAAAGFFNGLVITRLKVPPLIMTIATLALYRGLAEGISQAHSVRGY
                     PQWFYFVGQENLFGVPAQLWLLLIAIVIAGIALDRTTFGRTLYAIGNNETAARFSGLP
                     VDRVKLIIYTLSGLMAGLSAFVLVSRVTTTRSDMGLGYELDVIAAVVLGGTSIFGGVG
                     TIWGAVVGIAMIQLLKDGLALTGVKGDATIVVIGAVLILSTLVASSLQRRRDGV"
     gene            complement(1468..2454)
                     /locus_tag="VKU03_11770"
     CDS             complement(1468..2454)
                     /locus_tag="VKU03_11770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ71987.1"
                     /translation="MTAATLFRAEAEPRRLLGVLASQEALLAIAVIGLAIVVGLDNPR
                     FLATRNLSDVLLGNAYIAVAAVGMSMVIISGNIDISVGSLIGVLATLSGTLAVDGAPT
                     AVTWLAPLLVGIVVMGLQGVVIAYLRIPAIVVTLGMLSILKGGLISVTGGKWITDLPD
                     SFHLADIELFGFVPMPVFLMIVATLLAAWWMRYSARGRAIYAVGGNGEAARLCGISQR
                     RTVVMVFALHGFFAGIAALLYATQLRVIQSTVPPNLELTIITASVVGGVSILGGVGTV
                     IGSTLAAVLIAEIASALVFINVSPYWIRAVQGVLILVTVIADILRRRRMAGA"
     gene            complement(2451..3986)
                     /locus_tag="VKU03_11775"
     CDS             complement(2451..3986)
                     /locus_tag="VKU03_11775"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015668392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ71988.1"
                     /translation="MDQARSEASPPPLLSVRNIEKSFPGVRALSGVSFDVAKGEVHAL
                     LGENGAGKSTLIKIISGVFPPDRGEVIVDGRPVNLERPDDARRAGIATIYQELLLFPE
                     LTVAENVYMGHAPRAGLGRLDWRAMREKTGELLASLEIHDLKADRVVGALSVGNRQRV
                     EILRALSQDARILIMDEPTAALTEYDVTRLFDIVRKLKARGVAVIYISHRLDEIFAIA
                     DRVTVLRDGAHVATKRVADTDSAELVQLMVGRKIESLFPKITVPIGEPVLEIKDLERR
                     PLTKKISLTVRAGEIVGLAGLVGSGRSELAQTIFGVTPAEAGEIRIAGKKVEIRSPAQ
                     ARALGVAYVPEDRGTQGLVRPMTVLENFSLAALGKVAFGGFIDRAAERKLAADGVKRF
                     SVKASSLEQVAGKLSGGNQQKIVLGKWLANQPKLLILDEPTRGIDVGAKAEIHRLMGE
                     LAAQGLAILMISSELPEVLGMSDRVLVMREGRIVAEFSRAEATQEAAGAAMMGSQEAE
                     KAA"
BASE COUNT          767 a         1298 c         1300 g          655 t
ORIGIN      
        1 accggcgcga tcgccaccgg atcgttggcg gcgaagagaa tgccgtcgac gccgctggtg
       61 atgtaattgt cgatgaactt gatctgctcg tcgatcttcg cttcggtcgg cccgtccgtc
      121 ttcaccgtga cgttgccgag ctccttggcc gcttcctgca tgcccttcga cgtggcgttg
      181 aaatagccga tgccgatcag cttgggcacg tcgaccagcg agatcggctt gcccgccttg
      241 ttcggcggat tggtcggttg gcccccgtca tagggcttgg ccgccggcgc gggactggct
      301 tcattggccg agcaggcgag cggactgacc ggaaggtcgt ccgcgccgtc ccatcgtttt
      361 tccgctgcat aggccgaccc ggccagcagc gtcgcgctga gcagcgccat agcgaatcca
      421 agtttcatac ttttttcctc ccatgtttaa agccgcttca aagcggcagg aaactccgtc
      481 aaacgccgtc gcggcgcctc tggagcgaac tcgccactag agttgaaagg atcagcaccg
      541 cgccgatcac gacgatcgtc gcgtcgccct tgacgccggt cagcgccagc ccgtccttga
      601 gaagctggat catcgcaatg ccgaccaccg cgccccagat cgtgccgacg ccgccgaaga
      661 tgctggtgcc cccaagcacc accgcggcga tcacgtcgag ctcgtagccg aggcccatat
      721 cggagcgcgt cgtcgtcacg cgcgagacaa ggacaaaagc ggaaagcccc gccatcaagc
      781 cggataacgt atagatgatg agcttgacgc gatcgaccgg caggcccgag aagcgcgccg
      841 ccgtttcgtt gttgccgatc gcatagagcg tgcgcccgaa tgtggtgcgg tcgagcgcga
      901 ttccggcgat cacgatggcg atcagcagga gccaaagctg ggccggcacg ccgaaaaggt
      961 tttcctggcc gacgaaatag aaccattgag gatagccgcg caccgaatga gcttggctga
     1021 tcccctcggc gagcccgcga tagagggcga gcgtcgcaat cgtcatgatc agcggcggca
     1081 ctttcagccg agtgatgacg agaccgttga agaagcccgc cgccgcaccg acaagcaggc
     1141 agaaaacgat cgccaaaggg agaggaaaat cgaaattctt ccacgaatag ccgagcagga
     1201 tcgcgcagag cccgaccgtc gagccgaccg aaagatcgat gccgccagtg acgataatga
     1261 aggtcatcgg caaggcgatc aagccgacct cggtggtcag ccggccctga ttgagcagat
     1321 tgtcgatggt caagaaacga ttgttcaaga cgccgagaat gatcagcgcg acgacgagga
     1381 tcgccgcgag catcgtctcg tggcggatga gccaccacgg cgctgcgcgc ttggcggcgc
     1441 gggggagggg gatttcgctc atcgcgctca tgctccggcc atgcgccgac gccgcaaaat
     1501 gtcggcgatc acggtcacga gaatgagcac gccctggacc gcccggatcc aataaggcga
     1561 gacgttgatg aaaaccagcg cgctggcgat ctccgcgatc aaaaccgcgg cgagggtcga
     1621 gccgatgacg gtgccgacgc cgccgagaat gctgacgccg ccgaccaccg aagcggtgat
     1681 gatcgtcaat tcgaggttcg gcggcacggt cgactggatg acgcgaagct gcgtcgcata
     1741 gagcagggcc gcgatcccgg cgaagaagcc atgcagcgcg aagaccatga cgacggtgcg
     1801 gcgctgcgag atgccgcaaa gccgcgccgc ttcgccattg ccgccgaccg cgtaaatggc
     1861 gcggccgcgc gccgaatagc gcatccacca ggcggcgagc agcgtcgcca cgatcatgag
     1921 gaagaccggc atgggcacga aaccgaagag ctcgatatcg gcgagatgga aactgtcggg
     1981 cagatcggtg atccatttgc cgccggtgac gctgatcagg ccgcctttga ggatcgacag
     2041 catgccgagc gtcaccacga tcgccggaat gcgcaaataa gcaatgacga cgccttgcag
     2101 gcccatgacc acgatgccga cgagcagcgg cgccagccac gtgacggcgg tcggcgcgcc
     2161 gtccaccgcc aatgtgccgc tgagcgtcgc cagaacgccg atcagcgagc cgaccgaaat
     2221 gtcgatattg ccggagatga tcaccatcga catgccgacc gccgcaaccg cgatataggc
     2281 gttgccgagc agcacgtcgg aaaggttgcg cgtcgcgagg aagcgcggat tgtcgagacc
     2341 gacgacgatg gcgaggccga tcaccgcgat ggcgagcagc gcttcttgcg aagccaggac
     2401 cccgagcagg cggcgcggct cggcctcggc gcggaagagg gtcgccgccg tcatgccgct
     2461 ttctccgcct cttggctgcc catcatcgcc gcgccggcgg cctcctgcgt cgcttcggcg
     2521 cgcgaaaatt ccgcaacgat ccggccctcg cgcatgacga ggacgcggtc gctcatgccg
     2581 agcacttccg gcagctcgct cgaaatcatc aagatggcga ggccctgcgc cgccaattcg
     2641 cccatcaggc ggtggatttc cgccttggcg ccgacatcga tgccgcgcgt cggctcgtcg
     2701 agaatcagca gcttcggctg attggccagc catttgccga gcacgatctt ctgttggttg
     2761 ccgccggaga gcttgccggc gacctgttcg agcgaactcg ccttgaccga gaagcgcttg
     2821 acgccgtcgg cggcgagctt ccgttcggcg gcgcggtcga tgaagccgcc gaaggcgacc
     2881 ttgccgagcg cggcgaggct gaaattttca agcaccgtca tcggccgcac caggccttgc
     2941 gtgccgcgat cctcaggcac gtaggcgacg ccgagcgcgc gcgcttgcgc cggcgagcgg
     3001 atttcgactt tcttgccggc gatcctgatt tcgccggctt cggccggcgt cacgccgaaa
     3061 atggtttgcg ccagctcgct gcggcccgag ccgaccaggc cggcaaggcc gacaatctcg
     3121 ccggcccgca ccgtcaggct gatcttcttg gtgagcggcc gcctttcgag gtccttgatc
     3181 tccagaaccg gctcgccgat cggcacggtg attttcggga acaggctctc gatcttgcgg
     3241 ccgaccatca attgcacgag ctcggccgaa tcggtgtcgg cgacgcgctt cgtcgcgaca
     3301 tgggcgccgt cgcgcagcac cgtgacgcgg tcggcgatgg cgaaaatctc gtcgagccgg
     3361 tggctgatat agatgacggc gacgccgcgc gctttcagct tgcgcacgat gtcgaagagc
     3421 cgcgtcacgt cgtattcggt caaggcggcg gtcggctcgt ccatgatcag gatgcgcgcg
     3481 tcctgcgaca aagcgcgcag gatttcgacg cgctggcggt tgccgacgct gagcgcgccg
     3541 accacgcggt cggctttcag atcgtgaatt tcgagcgagg cgagcagctc cccggtcttt
     3601 tcgcgcatcg cccgccaatc gagccggccg agcccggccc gcggcgcatg gcccatataa
     3661 acgttctcgg cgacggtcag ctccggaaac agcagcagct cttggtagat cgtggcgatt
     3721 ccggcgcgcc tcgcgtcgtc gggccgctcc aaattgactg gccggccatc gacgatcacc
     3781 tcgccgcgat cgggcggaaa gacgccggaa atgatcttga tcaaagtcga tttgccggcg
     3841 ccgttctcgc cgagcaaagc atgcacttcg cctttggcca cgtcgaagga cacgccggac
     3901 aaggcccgaa cgcctgggaa ggacttttcg atgttgcgca ccgacagaag aggcggcggc
     3961 gaagcttcgc ttcgggcttg atccatcgcc gtcacgccgc cacgatcgta tcgacgccgg
//
LOCUS       DATGJW010000390         3691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_177514, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000390 DATGJW010000000
VERSION     DATGJW010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3691
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_177514"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..276
                     /locus_tag="VKU03_11780"
     CDS             <1..276
                     /locus_tag="VKU03_11780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893002.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ71989.1"
                     /translation="SSTNFSMFVFDNGLRLGLWRRGDVQPKPTAEPGGAEIIFPFEDA
                     KAVDAACAEWRGRGMPIAQEPTAMDFGYTFVALDPDGHRLRACALTT"
     gene            complement(335..1681)
                     /locus_tag="VKU03_11785"
     CDS             complement(335..1681)
                     /locus_tag="VKU03_11785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ71990.1"
                     /translation="MAKDAQGLELAGSPDSIAAFDRAVDDYFGWKGDPIGVLQGALDR
                     DPAFTLGASAIASLYALNGFRGDNAAVTQALAAAETSVGGASSREKRHLNAAKAWAAG
                     EIIRATNIWEDILVDHPADALALRFAHDSYFYLGHSLSIRDSVARVLPFWDPQNPNYG
                     YVLGAYAFGLEEAGELRRAEEVGRAAIARNAEDAWAVHAVAHVLETESRQGEGIDFLK
                     NSRASWGKAHALSIHNGWHLGLYLIEEGRFDEVLADYDRFVAPKIAGDSLLDLVDASA
                     ILWRLELAGADVGERWTPLARQWLTHVDDHVLVFNDLHIALAVSRANDSEGVARLRHS
                     LDRYERDGKGDNRDITVDVGRRIVDAALAFAEGDYRRVVDRILPVRYKVIRIGGSHAQ
                     RDLVTQTLIAAAERAGETNLARALLAERVALRPTERTKQAFARASGVTAGHSPLPA"
     gene            2199..2897
                     /locus_tag="VKU03_11790"
     CDS             2199..2897
                     /locus_tag="VKU03_11790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011155926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLJ71991.1"
                     /translation="MPTIALVDDDRNILTSVSLSLEAEGYRVQSYTDGASALDGLKTS
                     PADLAIFDIKMPRMDGMELLRRLRQKSDLPVIFLTSKDDEIDELFGLKMGADDFIRKP
                     FSQRLLVERVKAILRRANPKEAAAQKESEAKILERGALKMDPERHTCTWNNEPVTLTV
                     TEFLILQALATRPGVVKSRNALMDAAYDDQVYVDDRTIDSHIKRLRKKFKAVQSDFEM
                     IETLYGVGYRFKEV"
     gene            2950..>3691
                     /locus_tag="VKU03_11795"
     CDS             2950..>3691
                     /locus_tag="VKU03_11795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="stimulus-sensing domain-containing protein"
                     /protein_id="HLJ71992.1"
                     /translation="MSAQAQRDSAPAAPANVTQPRVRRHARRIWRRFVTRFSSSLTRR
                     ILVLNLGGLLVLVVAFLYLNQFRAGIIDARVQSLQTQGEIIAAAIAASAAVETDTITI
                     DPEKLLQLAPGQSSSPADADDQSIEFSINPERVGPVLRRLVMPTRTRARVYDRDGILL
                     LDSRALSSRDMIARSDLPGASTDSATIFERAWNAVKRRFAPIEPRRVEPLGGGKTPPE
                     VSSALLGHTQSVVRLNAKGETIVSVAVPI"
BASE COUNT          648 a         1205 c         1198 g          640 t
ORIGIN      
        1 agttcgacga atttctcgat gttcgttttc gacaacggct tgcggctcgg cctctggcgg
       61 cgaggcgacg ttcagcccaa gccgacggca gagccgggcg gcgccgagat catctttccc
      121 ttcgaggacg ccaaagcggt cgatgcggcc tgcgccgaat ggcgggggcg cggcatgccg
      181 atcgcgcaag agccgaccgc gatggatttc ggctacactt tcgtcgcgct cgaccccgac
      241 ggtcaccgcc tgcgggcctg cgcgctgacg acgtaaaagg gccgcacgcc ccttctcccc
      301 gccttgcggc gccttgcggg gagaagacaa tccgctatgc ggggagtggg gaatgtcccg
      361 ccgtcacgcc gctggcccgg gcgaacgctt gctttgtccg ctcggtcggc cgcagggcga
      421 cgcgctcggc gagcagcgcg cgggccaagt tggtctcgcc ggcgcgctcg gcggcggcga
      481 tcagggtctg ggtcacgagg tcgcgctggg cgtggctgcc gccgatcctg atcaccttgt
      541 agcggaccgg caggatccga tcgacgacgc ggcggtaatc gccctctgcg aaggccagag
      601 ccgcatcgac gatgcggcgg ccgacatcga cggtgatgtc gcgattgtcg ccctttccgt
      661 cgcgctcgta gcgatcgagg gagtggcgca agcgcgccac gccttcgctg tcgttggcgc
      721 gcgagacggc gagcgcgatg tgcagatcgt tgaagacgag cacgtgatca tcgacatggg
      781 tcagccattg ccgggcgagc ggcgtccagc gctcgccgac atcggcgccg gcgagctcca
      841 gccgccacaa aatggccgac gcgtcgacga ggtcgagcag ggagtcgccg gcgattttcg
      901 gcgcaacgaa acggtcgtaa tcggcgagca cctcgtcgaa acggccttct tcgatcaggt
      961 acaaaccaag atgccagcca ttatggatcg acaaggcatg cgccttgccc caggacgcgc
     1021 gcgagttttt gaggaagtcg attccttcgc cctgccggct ctcggtctcc agcacatgcg
     1081 cgacggcgtg cacggcccag gcgtcctcgg cgttgcgggc gatggcggcg cggccgacct
     1141 cttcggcgcg gcgcaattcg ccggcttcct ccaggccgaa cgcataggcg ccgagcacat
     1201 aaccgtaatt cggattctgc ggatcccaga aaggcagaac gcgggcgacc gaatcgcgaa
     1261 tgctcagcga atggccgaga tagaaatagc tgtcgtgggc gaagcgcaaa gcgagcgcgt
     1321 cggcggggtg gtcgacgaga atgtcctccc agatgttggt cgcgcggatg atctcgcccg
     1381 ccgcccaggc tttcgcagcg ttaaggtggc gcttttcgcg tgacgaggcg ccgcccaccg
     1441 aggtctctgc ggcggcgagc gcttgggtta ctgcggcatt gtcgccgcga aagccgttca
     1501 gcgcatagag cgaggcgatc gcgctggcgc ccagcgtgaa agccggatcg cgatccagcg
     1561 cgccttgcag cacgccgatc ggatcgccct tccagccgaa ataatcgtcg accgcgcggt
     1621 cgaaagcggc gatcgaatcc ggcgacccag ccagttcgag accttgcgca tccttggcca
     1681 tgcccggctc ctctgctgac gccttgcgat ggatgatagg gaaaagaagg acgatctgtc
     1741 tattattttt gtataaagat gtggctttga gcaaccgggc ttggcgagcg gatcgcgact
     1801 gtcgcgccga cccgcgacag cggccacgct ccgccgattg atgtcgccct ccgccctcgg
     1861 ttggaccgcc atttttatcg ccggtgaagg cgaatcggcc cgggctggcc aagacggcgc
     1921 gagtggtcta tgctaagtca atggccgatc tggcggaatc ggcgaggctt tcggcgactt
     1981 cgtcgacgcg ccgaatggtc gcggcagggc cgcaatcttg tcccgtttcc gccttgcgcg
     2041 ggaactttgt ccttgccgtc gaacttgttc gggcggaaaa agcgtcacat ttcggccttc
     2101 aacgctctta aagtcccggc gcgtccagtc gggccgagcg tctgacacaa caacgccgcg
     2161 ccgccccgcc ggctacgctg agtgaagatt ggagcctcat gccgacaatc gccttggtcg
     2221 acgacgaccg caatatcctc acctccgtgt cgctctctct ggaagcggaa ggctatcggg
     2281 ttcaatccta tacggacggc gccagcgcct tggacgggct caagaccagc cccgccgatc
     2341 tcgccatctt cgacatcaag atgccgcgca tggacgggat ggagttgttg cgccgcctga
     2401 ggcaaaagtc cgacctgccg gtgattttcc tcacctcgaa ggatgacgag atcgacgaat
     2461 tgttcggcct caaaatgggc gccgacgatt tcattcgcaa gccgttctct cagcgcctgc
     2521 tggtcgagcg ggtgaaggcg atcctgcgcc gcgccaatcc gaaagaggcg gcggcgcaga
     2581 aagagagcga agcgaaaatt ctcgagcgcg gcgctctcaa aatggacccg gagcgccata
     2641 cctgcacctg gaacaacgag ccggtgactt tgaccgtgac cgaatttctg atcctgcagg
     2701 cgctcgccac ccggccgggc gtggtcaaga gccgcaatgc gctgatggac gccgcttatg
     2761 acgatcaggt ctatgtcgac gaccgcacga ttgacagcca catcaagcgg cttcgcaaga
     2821 agttcaaggc ggtccaaagc gatttcgaga tgatcgagac gctctatggc gtgggctacc
     2881 gcttcaagga agtatagaag cgaagctggc gagcctgccg aaagggcaga ctcttcccgg
     2941 gacgcagcga tgagcgcgca agcgcaacgc gattctgcgc cggccgcgcc ggcgaatgtg
     3001 acgcagccgc gcgtccggcg gcacgcgcgc cgcatctggc gacgcttcgt gacgcgcttt
     3061 tcctcctcgc tgacccgccg cattctcgtc ctcaatctcg gcggcctgct cgtcctcgtc
     3121 gtcgccttcc tctatctcaa tcaatttcgc gccggcatta tcgatgcgcg ggtgcagagc
     3181 ttgcagaccc aaggcgaaat catcgccgcg gcgatcgccg cctccgcggc ggtcgaaacc
     3241 gacacgatca ccatcgatcc ggaaaagctg ctgcagctcg cgcccggcca gagctcttcg
     3301 cccgccgacg ccgacgatca gtcgatcgaa ttctcgatca atcccgagcg cgtcggcccg
     3361 gtgttgcgcc gcctggtcat gccgaccagg acgcgggcgc gcgtctatga ccgcgacggc
     3421 atcctgctgc tcgattcgcg cgcgctgtcc tcgcgcgaca tgatcgctcg ctccgacttg
     3481 ccgggcgcct cgaccgactc ggcgacgatt ttcgagcggg cctggaacgc ggtgaagcgg
     3541 cgcttcgcgc cgatcgagcc gcgccgcgtc gagccgctcg gcggcggcaa gacgccgccg
     3601 gaagtatcga gcgccctgct tggccacacc caatcggtgg tgcggctcaa cgccaagggc
     3661 gagacgatcg tttcggtcgc ggtcccgatc c
//
LOCUS       DATGJW010000391         5178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_904190, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000391 DATGJW010000000
VERSION     DATGJW010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5178
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_904190"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..342)
                     /locus_tag="VKU03_11800"
     CDS             complement(<1..342)
                     /locus_tag="VKU03_11800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004684894.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M81 family metallopeptidase"
                     /protein_id="HLJ71993.1"
                     /translation="MRIFTASLATETNTFSPAPTDRASFETAFYAGPGRHPPTPTLCS
                     APIVVLRRRAAEEGFTLIEGSAAWAEPGGLLQRKAYEELRDEILAQLRAALPVDGVVL
                     GLHGAMVAQGYD"
     gene            complement(418..783)
                     /locus_tag="VKU03_11805"
     CDS             complement(418..783)
                     /locus_tag="VKU03_11805"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006326662.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLJ71994.1"
                     /translation="MTRQHFGAAQVPLSPAVRAGDFIYISGQVPAGADGAIVQGGIDA
                     QTRQVMENIKAALALAGAELKDVIKTTAILADARDFGAFNKAYAAYFPKDPPARTTLE
                     ARLMIDIKVEIEAVAYKPA"
     gene            complement(795..1622)
                     /locus_tag="VKU03_11810"
     CDS             complement(795..1622)
                     /locus_tag="VKU03_11810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006133376.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLJ71995.1"
                     /translation="MKRRKLIRSLIFWALLSPIVVAILFPYAVMIITALKSPTEVHDP
                     TWWPHEWRWRNFPEMWQTSGFAPALVNSLYVSGLTTIGAVIVSIPAAYALSRFRFVGR
                     GAFSQFLLVSQMLSPIVLVIGLFRLVVNLGMIDSLNALTLIYVAFNIAFCVWMMQSYF
                     TTISRDLEEAAWLEGAGRMRTLIVVFLPLAAPAIAVTAIFAFINAWNEFVIALTMLRR
                     EDNYTLPIRVFSLVAGRYTVDWHYVMAAAFAASVPVAVLFAWLQRYLVRGLALGAVK"
     gene            complement(1619..2491)
                     /locus_tag="VKU03_11815"
     CDS             complement(1619..2491)
                     /locus_tag="VKU03_11815"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012093248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLJ71996.1"
                     /translation="MSRALTPYALILPSFLLAAVIVLWPVEEIVSLATHDVNRFGMLR
                     DFVGLANFRSLLTDPDFISSLWRTLIWTAGVVLGTLIVSTPIALILNEDFYGRGLARI
                     LIMLPWAVSLAMTAVIWRWALNGESGMLNSALRDLGLMNRNIQWLAQASTAFPMQILI
                     GILVSIPFTVTIFLGGLSSVPDDLYDAAAIEGAGGWDQFRLITLPLLRPFINIAFVLN
                     TIYVFNSFPIIWATTQGGPADSTDILVTYLYKLGFRLGRLGDAAAVSLIMFAILLAFT
                     ILYVRLAMREEAAT"
     gene            complement(2693..3922)
                     /locus_tag="VKU03_11820"
     CDS             complement(2693..3922)
                     /locus_tag="VKU03_11820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019443411.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ71997.1"
                     /translation="MKILRKALLVAGASLLLASAAEAGTVRVTIAEYSKGTGPYFDAA
                     AKAFEAANPGTKIQIEVVPWDNLQQKLTTDIAAGANADLSIIGTRWLLDYVSQGVVAP
                     LDSYVTPDFKARFIQTFLTPSIMNGHVYGLPIAASARAMYYNKDVFKKAGYDSAPGTW
                     ADFKNAAEKIKATGGGVFPFGLQGKEIETDVYFYYAMWSYGGEIVKDGKSGLDSQAAI
                     DAATLYKSFIDGGLTEPGVTSYNREDVQNLFKQGKVATVITAPFLSGQIKKEAPNLNY
                     AVAPIPAGPNGDQGTYGVTDSIVLFANSKVKDEAWKFLDFLFTTDERTKFDKVEGFLP
                     VNAEEAKNPIFADDRDLKVFASLLPKARFAPVIPGWEDIAQTTTNALQKVYLGQGQIE
                     PTLKEAAAKINETLSKK"
     gene            4141..4923
                     /locus_tag="VKU03_11825"
     CDS             4141..4923
                     /locus_tag="VKU03_11825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013892371.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ71998.1"
                     /translation="MTAKAAPFRLDGRRALITGAGGGIGRALVSAFKAAGATVIGADR
                     DESLLAGLEVDRRLAFDLTDSDAAAAAIERLEAEAAAPDVVVSNAGRTRAETLADVDK
                     AAWERELAVNLTGAFNIVSPALAAMQRRGGGSIVFIATVNALAHYGNPAYAAAKAGLV
                     AYAKAIAVERGAAGIRANVVCPGSVRTPAWDHRLRRTPDLLDSVLPHYPLGRLVTPEE
                     VASAALFLASDAASGITGAVLAVDAGLTAGNLRFVNDVLGRG"
     gene            4925..>5178
                     /locus_tag="VKU03_11830"
     CDS             4925..>5178
                     /locus_tag="VKU03_11830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017996489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ71999.1"
                     /translation="MASLSIRRLEKAYGPARVLHGVDLDVADGEFVVLVGPSGCGKST
                     LLRMIAGLDAASSGEMWIGGRRADALPPQQRNIAMVFQSYA"
BASE COUNT          966 a         1607 c         1700 g          905 t
ORIGIN      
        1 gtcatagcct tgcgccacca tggcgccgtg caagcccaga accacgccgt cgaccggcag
       61 cgcggcgcga agctgggcga ggatctcgtc gcgcaattct tcgtacgctt tgcgctgcaa
      121 gagcccgccc ggctcggccc aggcggccga gccttcgatc agcgtgaaac cctcctccgc
      181 cgcgcgccgg cgcagcacga cgatcggcgc cgagcacaga gtcggcgtcg gcggatgccg
      241 gcccggcccg gcgtagaagg cggtttcgaa actcgcccga tcggtcggcg ccggcgagaa
      301 cgtgttggtc tcggtcgcga gggaggcggt gaaaatgcgc atcggtcact tctgcatcaa
      361 tccgcggcct ctcccaagag ggggcgccgc cggctacgcc gtaccttcct ctcgaaatca
      421 ggccggctta taggccacgg cctcgatttc aaccttgatg tcgatcatca gccgcgcctc
      481 cagcgtggtg cgggccggcg ggtcttttgg aaaatacgcg gcataggctt tgttgaacgc
      541 cccgaagtcg cgcgcgtcgg cgaggatcgc cgtcgtcttg atcacgtcct tcagctccgc
      601 gccggcaagc gccagggcgg ctttgatatt ctccatcacc tggcgcgttt gcgcgtcgat
      661 tccgccctgg acgatcgccc cgtcggcgcc ggcggggacc tggccggaaa tatagatgaa
      721 gtcgccggcg cgcacggccg gcgaaagcgg aacctgggcg gcgccgaaat gctgtcgcgt
      781 catgtttcac atcctcattt gacggcgccg agcgccaagc cgcgcacgag atagcgctgc
      841 agccaggcga acagcacggc gaccggaacc gaggccgcga aagccgcggc catcacatag
      901 tgccaatcga cggtgtagcg gccggcgacc agcgagaaca cgcggatcgg cagggtgtaa
      961 ttgtcctccc gccgcagcat ggtcaaggcg atgacgaatt cgttccaggc gttgatgaag
     1021 gcgaagatcg ccgtcaccgc gatcgccggc gcggcgagcg gcaggaaaac gacaatcaag
     1081 gtgcgcatgc gccccgcgcc ctcgagccag gcggcctctt cgaggtcgcg cgaaatggtc
     1141 gtgaaatagc tctgcatcat ccagacgcag aaggcgatgt tgaacgcgac atagatcaag
     1201 gtcagcgcgt tgaggctgtc gatcatgccg agattgacga cgaggcggaa aagcccgatc
     1261 accagcacga tcggcgacag catttgcgag acgagcagga attggctgaa cgcgccgcgg
     1321 ccgacgaagc ggaagcggct gagcgcgtag gccgccggga tcgagacgat gaccgcgccg
     1381 atcgtcgtca ggcccgaaac gtagagggag ttgaccaaag ccggggcgaa gccggacgtc
     1441 tgccacattt cgggaaagtt ccgccagcgc cattcgtgcg gccaccaggt cggatcatgc
     1501 acttcggttg gcgatttcag ggcggtgatg atcatcaccg cataagggaa aaggatcgcc
     1561 acgacgatcg gcgacaaaag cgcccagaaa atcagcgagc gaataagttt gcggcgtttc
     1621 aagtcgcagc ctcttcgcgc atggcgagcc gcacatagag aatggtgaag gcgagcagga
     1681 tcgcgaacat gatcagcgag accgccgctg cgtcgccgag gcgccccagc cggaagccga
     1741 gcttgtaaag gtaggtcacc agaatgtcgg tcgaatcggc cggcccgccc tgcgtcgtcg
     1801 cccagatgat cggaaaagag ttgaacacat agatcgtgtt cagcacgaag gcgatgttga
     1861 tgaagggcct gagcaggggc agggtgatca gccggaactg atcccatccg ccggcgcctt
     1921 cgatggcggc ggcgtcatag agatcgtcgg gaaccgaaga aaggccgccg agaaaaatcg
     1981 tgacggtgaa cggaatcgag acgagaatgc cgatgaggat ttgcatcgga aaggcggtcg
     2041 aagcctgggc cagccattgg atgttgcggt tcatcaggcc aaggtcgcgc aaagccgaat
     2101 tgagcatgcc gctctcgccg ttcagcgccc agcgccagat caccgcggtc atcgccaggg
     2161 acacggccca gggcagcatg atgaggatgc gggcaaggcc gcgaccgtaa aaatcctcgt
     2221 tgaggatgag cgcgatcggc gtcgatacga tgagcgtgcc gagcacgacg ccggcggtcc
     2281 agatcaaggt gcgccagagc gagctgatga aatccggatc ggtcagcagc gagcggaaat
     2341 tggcgaggcc gacgaaatcg cgcagcatgc cgaagcggtt cacatcatgc gtcgccagcg
     2401 agacgatctc ctcgaccggc cagagcacga tcaccgccgc gagcaggaag ctcggcaaga
     2461 tgagagcgta aggggtgagg gcgcggctca tgacggcgac ggtttggtac ggtttacctg
     2521 atcgtcattg cgagcagcga gggggaggaa gcaatccatt cgtagggatg cgctttgtga
     2581 ttgcttctct tcgcccgcaa tgacggagag attgtagccg cggtcggcga ccgcggcgta
     2641 atgacgggaa gatggccgcg gtcggcgacc gcggctacag gggctcgcgc cgctacttct
     2701 tcgacagcgt ctcgttgatc ttcgccgccg cctctttcag ggtcggctcg atctggccct
     2761 ggccgagata gactttctgc agcgcgttgg tcgtcgtctg ggcgatgtcc tcccagccgg
     2821 gaatgaccgg cgcgaagcgc gccttgggca gaagcgatgc gaacactttc aaatcgcggt
     2881 cgtcggcgaa gatcggattc ttggcttcct cggcgttgac cgggaggaaa ccctcgacct
     2941 tgtcgaactt ggtgcgctcg tctgtggtga agaggaaatc gaggaatttc caggcttcgt
     3001 ccttcacctt ggaattggcg aagagcacga tcgaatcggt gacgccataa gtgccctgat
     3061 cgccgttcgg cccggccgga atcggcgcga cggcgtaatt gaggttcggc gcctctttct
     3121 tgatctggcc ggacaggaag ggcgcggtga tgacggtcgc gaccttgccc tgcttgaaga
     3181 ggttctgcac gtcctcgcga ttgtaggacg ttacgccagg ctccgtcagg ccgccgtcga
     3241 tgaagctctt ataaagcgtg gccgcatcga tcgccgcctg gctgtcaagg ccggatttgc
     3301 cgtccttcac gatctcgccg ccataggacc acatcgcata atagaaatag acgtcggttt
     3361 cgatctcttt gccctgcagg ccgaacggaa acacgccgcc gccggtcgcc ttgatcttct
     3421 cggccgcatt cttgaaatcg gcccaagtgc cgggcgccga atcgtagccg gccttcttga
     3481 acacgtcctt gttatagtac atcgcccgcg ccgaggcggc gatcggcagg ccatagacat
     3541 ggccgttcat gatcgacggc gtgaggaagg tctggatgaa acgcgccttg aaatccggcg
     3601 tgacatagga atcgagcggc gcgaccacgc cctggctgac atagtcgagc agccagcgcg
     3661 tcccaataat agagagatcg gcattggcgc cggcggcaat gtcggtggtc agcttctgct
     3721 gcagattgtc ccacggcacg acctcgatct gtatcttggt gccgggattg gcggcctcga
     3781 acgccttcgc cgccgcgtcg aaataagggc cggtgccttt ggaatattct gcgatcgtca
     3841 cgcgcaccgt gccggcctcg gcggcgctcg ccagcaacaa gctcgcgcct gcgacaagaa
     3901 gggccttccg caagattttc atcgtttcgc tcccataaga cgcgttcgcc gtccaaggct
     3961 ttgccggttt ttccgcatcg ccgcctctgc caagcaaccg gcggcgcgcg gccacttagt
     4021 gttgtatgta atttagtttc gcgcaagcgt gatttttgac ctcgcttaag cggattccgt
     4081 gttgcttttt ttcatcgttc agattttgct gaagacgact ggcgaggcga gagaacgaca
     4141 atgacggcca aggcggcgcc ttttcggctc gacggccgcc gcgctctgat taccggggcc
     4201 ggcggcggca tcggccgcgc cttggtctcg gccttcaaag cggcgggcgc gactgtgatt
     4261 ggcgccgatc gcgacgagag cctgctggcc gggctcgaag tcgatcgccg cctcgccttc
     4321 gatctgaccg acagcgacgc ggccgccgcg gcgatcgagc ggctcgaagc ggaggcggcc
     4381 gcgccggacg tcgtcgtcag caatgccggc cggacgcgag ccgagacttt ggccgatgtc
     4441 gacaaggccg cctgggagcg cgagctcgcc gtcaacctga ccggcgcctt caacatcgtc
     4501 tctcccgcgc tcgccgcgat gcagcggcgc ggcggcggtt cgatcgtttt catcgccacc
     4561 gtcaacgcgc tcgcccatta cggcaatccg gcctatgcgg cggcgaaggc gggccttgtc
     4621 gcctacgcca aggccatcgc cgttgagcgc ggcgcggcgg ggattcgcgc caatgtcgtc
     4681 tgccccggtt cggtacgaac gcctgcctgg gaccaccgcc tgcgaagaac gccggacctc
     4741 ctcgacagcg tcttgccgca ttatccgctc ggccggctgg tgacgcccga agaggtcgcc
     4801 agcgccgcct tgttcctcgc ctccgacgcg gcgtccggca taaccggcgc ggtcctcgcc
     4861 gtggacgccg gcctcaccgc gggcaatctc cgtttcgtca acgacgttct cggtcgaggc
     4921 tgacatggcc tcgctcagca tccggcgctt ggagaaggcc tatggtccgg ctcgcgttct
     4981 gcacggcgtc gatctcgacg tagccgacgg cgaattcgtc gttctggtcg ggccgtcagg
     5041 ctgcggcaaa tccacgctgc tgcgcatgat cgccggcctc gacgcggcct cgtccggcga
     5101 aatgtggatc ggcggcaggc gcgccgacgc cttgccgccg cagcagcgca acatcgccat
     5161 ggtgttccag agctatgc
//
LOCUS       DATGJW010000392         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1112316, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000392 DATGJW010000000
VERSION     DATGJW010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1112316"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VKU03_11835"
     CDS             <1..620
                     /locus_tag="VKU03_11835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HLJ72000.1"
                     /translation="VYDTYEIERISRVAFDLARKRRNKVTSSEKRNVMKSGVLWNEVV
                     TALHKREYSDVALEHQLADALGMQLVRRPKQFDVIVTDNLFGDMLSDVAAMLTGSLGM
                     LPSASLGEVNPKTGKRKALYEPVHGSAPDIAGKGLANPLAMIGSFGMALRYSFGLNEA
                     ADRLDAAISAALSAGLRTADIQAPGSVVVSTAQMGDAIVAELSKG"
     gene            complement(711..1466)
                     /locus_tag="VKU03_11840"
     CDS             complement(711..1466)
                     /locus_tag="VKU03_11840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72001.1"
                     /translation="MDAGWAEPGALPSEAALGGLDDWDRRRPRRRGAAPRRVLLAAFA
                     ASLLAMGGAMEFFPAARRGAEAPATISAAPAREFQPRNDLPPLLYVKALDGGQAAAAY
                     EAETRASDGARKDALTLGDPFSDGPFLRASLRIGGAATPPPLFFVEIARQAAEIGLAV
                     GRASDPPANGAPILYSDVALDAAGAERACLGFRFNSRSPVDLSGLACGGAGQPLDRAS
                     LECLISRIAPTAAGAAAGLDRLNGLAAGGKSGC"
     gene            1680..>2251
                     /locus_tag="VKU03_11845"
     CDS             1680..>2251
                     /locus_tag="VKU03_11845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HLJ72002.1"
                     /translation="MTLGGPAANLIGSALEADAPSSPVVRFGLDRPLRLDAGVDLSPL
                     TIAYQTYGALNAAKSNAFLICHALTGDQHVANRNPVTGKSGWWETMVGPDKPIDTNRF
                     FVICANVLGGCMGTTGPASTNPATGRPYELDLPLVTIADMVRAQAMLIDHLGIETLFC
                     VAGGSMGGMQVLQWAALYPERVFSALPIATA"
BASE COUNT          390 a          763 c          731 g          367 t
ORIGIN      
        1 aggtctatga cacctacgaa atcgagcgca tttcccgcgt cgccttcgac ctcgcgcgca
       61 agcggcgcaa caaggtgacc tcgtccgaaa agcgcaacgt gatgaagtcc ggcgtgctgt
      121 ggaacgaagt cgtcacggcc ctgcacaagc gcgaatattc ggacgttgcg ctcgagcacc
      181 agctcgccga cgcgctcggc atgcaattgg tgcgcaggcc gaagcaattc gacgtgatcg
      241 tcaccgacaa tctcttcggc gacatgctgt cggacgtcgc ggccatgctg accggctcgc
      301 tcggcatgct gccctccgcc tcgctcggcg aggtcaatcc gaagaccggc aagcgcaagg
      361 cgctctacga gccggtgcat ggatcggcgc ccgacattgc cggcaagggc ctcgccaatc
      421 cgctcgccat gatcggctcg ttcggcatgg ctttgcgcta ttcgttcggc ctcaacgagg
      481 cggcggaccg gctcgacgcc gccatctcgg cggcgctcag cgcgggcctg cggacggccg
      541 atattcaagc gcccggcagt gtggtggtct ccaccgcgca gatgggcgac gcgatcgtcg
      601 cggagctgag caaaggttga cgcggcgctg tgtgaacttc gtgcgtcttt gctggactcc
      661 ctgttgaatt tcgcacgaag ttcgccagga cgcacaaaga tcacgaagaa tcagcagccg
      721 gatttgcctc ctgccgcgag cccgttaagt cggtcgagcc cggccgccgc gccagcagca
      781 gtcggcgcaa tccgcgaaat caggcattcg agcgaagcgc ggtcgagcgg ttggccggcg
      841 ccgccgcaag caagcccgga aagatcgacc ggggagcggc tgttgaagcg gaagccgagg
      901 caggcgcgct ccgcgcccgc ggcgtcgagc gccacgtctg aatagaggat aggcgcgccg
      961 ttcgccggcg gatcggaggc gcgccccacg gccaggccga tttcggccgc ttgccgcgca
     1021 atctcgacga agaacagagg cggcggcgtc gccgcgccgc cgatccgcaa agaggcgcga
     1081 agaaagggac cgtccgaaaa tgggtcgcca agcgtcagcg cgtcctttct cgcgccgtcg
     1141 ctggcgcgcg tctcggcttc ataggccgcc gccgcctggc cgccatcaag cgctttgaca
     1201 tagagcagag gcggcaagtc gttgcgcggc tgaaattctc gcgccggcgc ggcgctgatc
     1261 gtcgccggcg cttcggcgcc gcgtcgcgcg gcggggaaaa attccatcgc cccgcccatg
     1321 gccaacaacg acgccgcaaa tgcggccagc agcacgcgcc gcggcgccgc cccgcgccgc
     1381 cgcggccgtc gccgatccca gtcgtcgagg ccgcccaaag ccgcctcgga aggcagagcg
     1441 ccgggctcgg cccatcccgc atccattggt tttgcgatcg ttccgaccgc tcccggtttc
     1501 tttcgatttt agcggagggc cggccgattg gggttagcgc gccggcaaag attgggaaga
     1561 tcgccaatgt ctggttaaac cgagaggctt cgcccttaag gctcagcatt ttccgcttga
     1621 attcggcgta tattccatat aacaaacgat acgttcgaaa gacagaacgt atcggaggcg
     1681 tgacgttggg cggtcccgcg gcaaatctca tcggcagcgc cctggaggcg gacgcgccga
     1741 gcagccctgt cgtccggttc gggctggacc gtccgctgcg cctcgacgcc ggcgtcgatc
     1801 tctcgccgct gaccatcgcc taccagacct atggcgcgct caacgcggcc aagtccaacg
     1861 ctttcctcat ctgccacgct ttgaccggcg atcagcacgt cgccaaccgc aatccggtca
     1921 ccggcaagag cggctggtgg gagacgatgg tcggcccgga caagccgatc gacacgaacc
     1981 gcttcttcgt catctgcgcc aatgtgcttg gcggctgcat gggcacgacc ggcccggcct
     2041 cgaccaatcc ggccacaggt cggccctatg aactggattt gccgctggtc accatcgccg
     2101 acatggtgcg cgcgcaggcg atgctgatcg accatctcgg catcgagacg ctgttctgcg
     2161 tcgccggcgg ctcgatgggc ggcatgcagg tgctgcaatg ggcggcgctc tatcccgagc
     2221 gcgtgttcag cgcgctgccg atcgccaccg c
//
LOCUS       DATGJW010000393         9906 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_485045, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000393 DATGJW010000000
VERSION     DATGJW010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9906)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9906)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9906
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485045"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..400
                     /locus_tag="VKU03_11850"
     CDS             <1..400
                     /locus_tag="VKU03_11850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72003.1"
                     /translation="SLLWRATREARLALTASTAQARRASRCVHAVALRGGGKERSLIL
                     GISSILCGFESKRKTFSARTDVGARGVFWSAGMSRLFHPVTIEQGPPRQADQASRETV
                     MAQELKGSAFNILFTASFFASFAYFMLHFG"
     gene            complement(381..1103)
                     /locus_tag="VKU03_11855"
     CDS             complement(381..1103)
                     /locus_tag="VKU03_11855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HLJ72004.1"
                     /translation="MKTDPLFREDAYLREAEAIVAAIEPGGIVLDRTVFYPQGGGQPG
                     DRGALVLDDGSRLAVANTVYGADRATILHVLADGAPLPALGARVLARIDWNLRFKRMR
                     AHSALHLLTAVLPYPVTGGAVGEAEGRLDFDSGEAALDKGEIAEKLNALIGQDAAVRT
                     RWISDDELVANPGLVKTMSVKPPTGTGRVRLVEIEGIDLQPCGGTHVLRTAEIGLAAV
                     TGIEKKGRINRRVRIALSQSEA"
     gene            complement(1100..2131)
                     /locus_tag="VKU03_11860"
     CDS             complement(1100..2131)
                     /locus_tag="VKU03_11860"
                     /EC_number="2.5.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine synthase A"
                     /protein_id="HLJ72005.1"
                     /translation="MKFSPSVVEAIGATPLIKLRRASEETGCTILGKAEFMNPGGSVK
                     DRAALAIIQDAIKSGALREGGVIVEGTAGNTGIGLCVVGSALGFRTVIVIPETQAQEK
                     KDMLRLVGAELIEVPAVPYANANNYVRYSGRLAEALAKSEPKGAIWANQFDNVANRRG
                     HYETTGPEIFADTDGKVDGFACAVGTGGTLAGVGLALKERNPKIKIALADPMGASLYE
                     FYTHGALKAEGSSITEGIGQVRITANLEGAPVDMAFQIPDEEALPIVFDLAQKEGLLL
                     GGSSGVNIAGAIRLAKAMGPGHTIVTILADSGMRYASKMFNPAFLRSKNLPVPAWLER
                     KPSAPSVFV"
     gene            complement(2187..2951)
                     /locus_tag="VKU03_11865"
     CDS             complement(2187..2951)
                     /locus_tag="VKU03_11865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007605473.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLJ72006.1"
                     /translation="MLYVISGFAVGVIVGLTGVGGGSLMTPILVLLFGIHPATAVGTD
                     LLYAAATKTAGTAIHGFNRAIVWRLTGLLALGSAPTAILMIFLLDHFGPFGADASLLI
                     TRLLGCALLLTALAMVLRRRLQAFAEKRAGKISPAARAALTVLTGAALGVFVSLSSVG
                     AGALGVTALVFLYPELPTKQIVGSDIAHAVPLVLVAGFGHWAIGSVDIATLGLLLTGS
                     IPGIILGSLAAPRLPELWLRPILALVLAVVGVRLIF"
     gene            3078..3899
                     /locus_tag="VKU03_11870"
     CDS             3078..3899
                     /locus_tag="VKU03_11870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020184628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdcF family protein"
                     /protein_id="HLJ72007.1"
                     /translation="MAGVLDRPMFFILSKALWSLVAPLNVLLVGGFIGALAASGRRFA
                     QLGRAVAIACLAILILAGVLPGSVLLIRPLEDRFPLPPKDMPPPAGVIVLGGAILADI
                     GEKRGEATIGEGAGRLIEAAILARRYRDARIVFTGGSNSLIARDSKEGEQAKRLFVEL
                     GVAPERILLETRSRNTDENARFTRALVSPKPGEVWLLVTSAYHMPRAMGVFRHAGFDV
                     VAYPTDYHTFGDSRDWRLLSETPPRLMLFDIAVHEWIGLIAYRLAGKIDDWLPRP"
     gene            complement(3938..4225)
                     /locus_tag="VKU03_11875"
     CDS             complement(3938..4225)
                     /locus_tag="VKU03_11875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562909.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3253 domain-containing protein"
                     /protein_id="HLJ72008.1"
                     /translation="MQSKPSAPDSATIEEAILSLCRAEGARTICPTDAAKAHAAARCE
                     DELAWRSHLSEVRRAAVRLAEAGRLVIYRKGKPADPHDFRGVYRLGLPRLD"
     gene            4331..4984
                     /locus_tag="VKU03_11880"
     CDS             4331..4984
                     /locus_tag="VKU03_11880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006609004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase family protein"
                     /protein_id="HLJ72009.1"
                     /translation="MPLRLVIANKAYSSWSLRPWILLAHFKIPFEEIVIPLDQPDTRK
                     RILAYSPNGKCPSLQDGPISVWESTAIIDYVAEKYPDKAIWPKAKAARAHARSLAAEM
                     HAGFQALRNHCPTNFRRPVRKRELTSEVEADVARLEAAWAGARKEHGKAGPFLFGRFS
                     AADAMFAPVVNRFHTYDVPVKKGTRAYMEAVMALPAWKAWIKDAEAEPWRIERYETI"
     gene            4954..5892
                     /locus_tag="VKU03_11885"
     CDS             4954..5892
                     /locus_tag="VKU03_11885"
                     /inference="COORDINATES: protein motif:HMM:NF036967.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HLJ72010.1"
                     /translation="MADRTLRDHLNGPLDRLRHAIGGKRARPRVTRIGGRRFIAQGLE
                     TNLWTDFYFNAMTVSWPRFFAWLAAIFVFINLVFAEIYSFGDAPIANAKKGSFSDLFF
                     FSVETTSTVGYGDMHPQTMYGHLVATAENFVGMVSLAVMTGLVFARFSRPRARLIYAD
                     NPVITRHNGLLTMMVRVANARNNFITDASAKLWLIRGEESSEGRRMTRFLPLRLERNE
                     NPVFALTWTLLHVIDEDSPLYGMSAEAIAASEMNFVVTINGLDETSAQLVHSRKPYPA
                     QSLRVGHEFVDVISIDEDGMRRIDYGKLHETRPAAI"
     gene            6074..7135
                     /locus_tag="VKU03_11890"
     CDS             6074..7135
                     /locus_tag="VKU03_11890"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HLJ72011.1"
                     /translation="MAAKLRVGFVGLGRVFDLNVRGYLGHPEAEVAALCDSDTDLLAR
                     RAAEHPNAVATRNFEEFLRADLDIVEILTPHPLHEAMAVAALERGAHISVQKPMAMTL
                     QECDRMIAAADRSGRRLKVFENFLFYPPLVRARQLLRDGAIGKPLHFRMKVVMADRAQ
                     AWTVPENASRWRHALAAAGRGGPMVFDHGHHMMAVALWLFGDLREGYACIESTETPAG
                     VYDAPATLTWRHADPPVHGVWDVSLALKMTLRTDYYADAERFEIQGEEGILQVTRCSD
                     RMLDEPVLTLYRDGEVRAFHNLETDWAASFPRSTRHFLDVIAGREAQQVLTGREGRRV
                     IAMYDLFQRSNAERRALTA"
     gene            complement(7157..7993)
                     /locus_tag="VKU03_11895"
     CDS             complement(7157..7993)
                     /locus_tag="VKU03_11895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="N-acyl homoserine lactonase family protein"
                     /protein_id="HLJ72012.1"
                     /translation="MPQAAQWEVYVMEFARSKNQPWVDLISGMYKEGVMDLPFSFILA
                     RCGGKNVLIDTGFMQESHGSSFSRKFGIPTWISPLRMLAEMSIGPDDVEDIFITHAHF
                     DHMGSIAEFPKAQIHIQKSELLSWYEAIALPKRFGHLTAIIDPDNLRTALDASIAHRV
                     TLIDGDKNDVLPGIHVRLGSGHTIGQQFVLLETAAGRRVISGDCVYSRRQFTGHGDDG
                     VYVPLNNAVGSVWEQLKTIDKMNDELGGDLSRLVVLHDIERWKGSPVVKEVEGFRIVR
                     IA"
     gene            complement(8007..9002)
                     /locus_tag="VKU03_11900"
     CDS             complement(8007..9002)
                     /locus_tag="VKU03_11900"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72013.1"
                     /translation="MSDTSVATRRLPPAVKVGLSVFSRFATIFGLLLMILVFSILSPD
                     AFPTYSNFINVLNQASLAMIIAGGLTLAVIVGELDLSIGYAASLHGVLVTGLIVQTKL
                     PVAVALMAVLALGGAIGVVNGLIVTKLRVNSVIATLGVGTIIVGLSFAYSAGVPIVAG
                     VPESFLQLALGRWLFGVPNPIIVMALVVGALWVLVERTPIGQEIQAAGANPVAARLAG
                     IGVDRIKILGFVISGVCAALTGALLASRLGSGTTSAADSYLLTAFAAVFLGSATLRDG
                     EFHVLGTFIGTLIIVFGFNGLNIFGAPTFSQYIFQGAILIVAVGLSSLGRVLAES"
     gene            complement(8999..>9906)
                     /locus_tag="VKU03_11905"
     CDS             complement(8999..>9906)
                     /locus_tag="VKU03_11905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008877170.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72014.1"
                     /translation="LELCQRVTVFRDGQSVAELDGAGLTRTGLVEAIVGRTVETAKAP
                     LSAPPRGKVVLAVRGFARPPKVRGVSFDLHRGEVLGLGGLVGAGRSELARLLFGADRP
                     DRGRATLDGQPFAPRDCAAAVKAGLGFVPEERRSEGLVLSKSVAFNLSLANLQKLLLV
                     GRLPLISTRKRAQMAAKIVRDLAIRTADVDAPVGRLSGGNQQKVVIGRWLQAAPKALI
                     LDEPTRGVDVGARAEIHRLVRELASRGMAVLVVSSEPEELPDLCDRVLVMAEGRIVAE
                     LIGESLTRERIIAASYAEAPQQTAA"
BASE COUNT         1771 a         3212 c         3261 g         1662 t
ORIGIN      
        1 gagcctgctt tggcgagcga cgcgcgaagc gcgcttggcc ttgacggcga gcacggctca
       61 ggcacgccgc gcttcaagat gcgtgcatgc cgtagcgctt cgtgggggag gaaaggaaag
      121 aagcctaatt ctcggaatat cttcaatttt atgcggattc gaatcaaagc gaaaaacgtt
      181 ctctgcgaga acagatgtcg gcgcgagggg cgtcttttgg agcgcaggaa tgagccggct
      241 ttttcatccc gtaaccatcg agcaagggcc gccgcgccag gcggaccaag cttcgcggga
      301 gacggtcatg gctcaggaac tcaaaggctc cgctttcaac attctcttca ccgccagctt
      361 cttcgccagc ttcgcttact tcatgcttca ctttggctga gcgcgatccg cacccggcgg
      421 ttgatccggc ctttcttctc gatgccggtc accgccgcca agccgatctc ggccgtgcgc
      481 agaacatgag tgccgccgca tggctgaaga tcgatcccct cgatctcgac cagtctgacc
      541 cggccggtgc cggtcggcgg cttcaccgac atggtcttga ccagacccgg attggcgacg
      601 agctcgtcgt ctgaaatcca gcgcgtgcgg accgcggcgt cctggccgat cagcgcattc
      661 agtttctcgg cgatctcgcc cttgtcgagc gcggcctcgc cggaatcgaa atcgagccgg
      721 ccttccgcct cgccgacggc gccgcccgtc accgggtagg gcaggacggc ggtcagcaga
      781 tgcagcgccg agtgggcgcg catccgcttg aagcgcaaat tccaatcgat gcgcgcgagg
      841 acgcgcgcgc ccagcgctgg caaaggcgct ccgtcggcga ggacgtggag gatcgtcgcg
      901 cgatcggcgc catacaccgt attggcgacg gccagacggg agccatcgtc gaggacgagc
      961 gcgccgcgat cgcccggctg gccgccgcct tgcggataga acacggtgcg gtcgaggacg
     1021 atgccgcccg gctcgatcgc cgcgacgatc gcctccgcct cgcgcagata ggcgtcttcg
     1081 cggaagagag gatcggtctt cacacgaaca cgctcggcgc gcttggcttg cgctcgagcc
     1141 aggcgggaac cgggaggttc ttcgagcgca gaaaggccgg gttgaacatt ttcgatgcgt
     1201 aacgcatgcc ggaatcggcg aggatcgtca cgatcgtatg gccaggcccc atcgccttgg
     1261 cgagccggat cgcgccggcg atgttgacgc ccgacgagcc gccgagcagc agcccttcct
     1321 tctgggcgag atcgaagacg atcggcagcg cctcctcgtc cggaatttgg aaggccatgt
     1381 cgaccggcgc cccttcgaga ttggcggtga tgcgcacttg gccgatgccc tcggtgatcg
     1441 aggagccctc cgctttcagc gcgccatgcg tatagaactc gtagagcgaa gcgcccatcg
     1501 gatcggcgag ggcgatcttg atcttcgggt tgcgctcctt caaagcgagg ccgacgccgg
     1561 ccagcgtgcc gcccgtgccg accgcgcagg cgaaaccgtc gaccttgccg tccgtatcgg
     1621 cgaagatttc cggcccggtt gtctcataat ggccgcggcg attggcgaca ttgtcgaatt
     1681 gattggccca gatcgcgcct ttcggttcgc tcttggcgag ggcttcggcg agccggccgg
     1741 aatagcgcac ataattgttg gcgttggcgt aaggaacggc cggcacttcg atgagctcgg
     1801 cgccgaccag gcgcagcatg tccttctttt cctgcgcctg agtctcgggg atgacgatga
     1861 cggtgcgaaa gccgagcgcc gagccgacca cgcagaggcc gatgccggta ttgccggccg
     1921 tgccctcgac gatcaccccg ccttcgcgca gcgcgccgct cttgatcgcg tcctggatga
     1981 tggcgagcgc ggcgcggtcc ttcaccgagc cgccgggatt catgaattcc gccttgccga
     2041 gaatcgtgca gccggtctcc tccgaagcgc gcctgagctt gatgagcggc gtggcgccga
     2101 tcgcttcgac gacggatggg ctgaatttca tggcgttcct cttgaggccc aggctttaag
     2161 cgatgggccg gggggagaca aggcggtcag aagatcagcc gcacgcctac cacggcgaga
     2221 accagggcca gaatcgggcg aagccagagt tccggcaggc gcggcgcggc caaactgccg
     2281 aggatgatgc ccggaatcga gccggtcagc aaaaggccga gtgtcgcaat gtcgacggag
     2341 ccgatcgccc aatggccgaa gcccgcaaca aggacgagcg gcacggcatg ggcgatgtcg
     2401 ctgccgacga tctgcttggt cggcagttcg ggatagagaa agacgagggc ggtgacgccg
     2461 agcgcgccgg cgccgaccga ggagagcgag acgaaaacgc caagcgcggc gccggtcagc
     2521 accgtcagcg cggcgcgcgc ggcgggcgag atcttgccgg cgcgcttttc ggcgaaggcc
     2581 tgcaggcggc ggcgcagaac catcgccagc gcggtcagca acagggcgca gccgagcagg
     2641 cgcgtgatca acaaggacgc gtccgcgccg aacggcccga aatggtcgag cagaaagatc
     2701 atcaggatcg cggtcggggc gctgcccagc gcgagcaggc cggtcagccg ccagacaatg
     2761 gcgcggttga agccatggat ggcggttccg gcggtcttgg tcgccgccgc atagagaaga
     2821 tcggtgccga cggcggtggc ggggtgaatg ccgaaaagca ggaccagaat cggcgtcatc
     2881 agcgaaccgc cgccaacccc ggtgaggccg acaatgacgc cgacggcgaa tcccgaaatg
     2941 acgtagagca agcccaagtc cccgcgccgg cgccgtttgg ccgcggaggg agatattcag
     3001 ctatagtcta taaagtcaat agactgttcc cctcgggccg ggaagccgta agcgccgcgc
     3061 agggcagggc gtttctgatg gcgggagttc tggatcgtcc gatgttcttc atcctttcca
     3121 aggcgctttg gtccctggtc gctcccctca acgtcctcct ggttggcggg ttcatcggcg
     3181 cgcttgccgc gtcgggtcgc cgcttcgcgc agctcgggcg ggccgtggcg atcgcctgcc
     3241 tcgccatcct gatccttgcc ggcgttctgc ccggctcggt cttgcttatc cgcccgctcg
     3301 aggaccgctt tccgctgccg ccgaaggaca tgccgccgcc ggcgggcgtc atcgtcctcg
     3361 gcggcgccat tctcgccgat atcggcgaaa agcgcggcga ggcgacgatc ggcgaaggcg
     3421 ccggccggct gatcgaggcg gcgatccttg cgcgccgcta tcgggatgcg cgcatcgtct
     3481 tcacgggcgg aagcaattcg ctgatcgccc gcgattccaa ggaaggcgaa caggccaagc
     3541 gcctcttcgt cgaattgggc gtcgcgccgg agcgcattct gttggagacc cggtcgcgca
     3601 acaccgacga aaacgcccgc ttcacccggg ctctcgtttc gccgaaaccc ggagaggtct
     3661 ggctgctcgt cacctcggcc tatcacatgc cgcgcgccat gggcgttttt cgccatgccg
     3721 ggttcgacgt cgtcgcctat ccgaccgact atcatacgtt cggcgacagc agggattggc
     3781 ggctcctttc cgagacgccg ccccggctga tgctgttcga catcgccgtc catgaatgga
     3841 tcggccttat cgcctatcgc ctcgccggca agatcgacga ctggcttcct cgaccctgaa
     3901 ccgcgctgta gccgcggtcg ccgaccgcgg ctacaggtca gtcgaggcgc ggcaatccga
     3961 ggcggtagac gccgcggaaa tcgtgtggat cggccggctt gcctttgcgg tagatgacca
     4021 gccgtccggc ctcggcgagg cgcaccgcgg cgcgcctcac ctcgctcaaa tgcgagcgcc
     4081 aggcgagctc gtcctcgcat cgcgcagcgg catgggcttt ggccgcgtcg gtcggacaga
     4141 tcgtgcgcgc gccttcggcg cggcagagcg ataggatcgc ctcttcgatg gtcgcggaat
     4201 caggcgcaga cggtttcgat tgcatggcgc cgagatgcgg agccgcgctt ctttcgtcaa
     4261 gcctttggct tgactttgat ccgccgcgac gctgaatgag cggaacaatc tctcttccgt
     4321 tggggtcttc atgccgctga gactggtgat cgccaacaag gcctattcgt cctggtcgct
     4381 gcggccctgg atcctgctgg cgcatttcaa aatccctttc gaggaaatcg tcattcccct
     4441 cgaccagccc gatacgcgca agcgcatcct cgcttattcg ccgaacggca aatgtccgtc
     4501 cttgcaggac gggccgatct cggtttggga atcgaccgcc atcatcgact acgtcgccga
     4561 gaaatatccc gacaaggcga tctggccgaa agccaaggcg gcgcgcgcgc acgcccgttc
     4621 gctcgccgcc gagatgcatg ccggcttcca agccttgcgc aatcattgcc cgaccaattt
     4681 ccgccggccg gtcaggaagc gcgaactgac gagcgaggtc gaggcggacg tggcccgcct
     4741 cgaagcggcc tgggccgggg cgcgcaagga gcacggcaag gcaggtccgt tcctgttcgg
     4801 ccgcttcagc gccgccgacg cgatgttcgc gccggtcgtc aaccgcttcc atacctatga
     4861 cgtccctgtg aaaaaaggga ctcgcgccta tatggaggcg gtcatggcgc ttcccgcctg
     4921 gaaggcctgg atcaaggacg ccgaagccga gccatggcgg atcgaacgct acgagaccat
     4981 ctgaacgggc cgctcgatcg cctccgccac gcgatcggcg gcaagcgggc gcggccgcgc
     5041 gtcacccgga tcggcggtcg gcgcttcatc gctcaagggt tggaaaccaa cctctggacc
     5101 gacttctact tcaacgccat gacggtgagt tggccgcgat tcttcgcctg gctcgccgcg
     5161 atcttcgtct tcatcaatct ggtcttcgcg gaaatctact cgttcggcga cgcgccaatc
     5221 gccaacgcca agaagggcag tttctccgac ctcttcttct tcagcgtcga gacgacgtcg
     5281 acggtcggct atggcgacat gcatccccag accatgtacg ggcatctcgt cgccacggcc
     5341 gagaatttcg tcggcatggt gtcgctcgcg gtgatgaccg ggctcgtctt cgcccggttc
     5401 tcgcggccgc gggcgcggct catctatgcc gacaatccgg tcatcactcg gcacaacggg
     5461 cttttgacga tgatggtgcg cgtcgccaac gcccgcaaca atttcatcac cgacgccagc
     5521 gccaagctgt ggctcatccg cggcgaagaa tcgagcgaag gacgccgcat gacgcggttc
     5581 ttgccgctgc gcctcgagcg caacgagaac ccggtcttcg ccttgacctg gactctcctt
     5641 cacgtcatcg acgaggacag tccgctctac ggcatgagcg cggaggcgat cgcggcgagc
     5701 gagatgaatt tcgtcgttac catcaacggg ctcgacgaaa cctcggcgca gcttgtccat
     5761 tcgcgcaagc cttatccggc gcaaagcctc agggtcggcc atgaattcgt cgacgtgatc
     5821 agcatcgacg aagacggcat gcggcgcatc gactacggca agctgcacga gacgcggccg
     5881 gcggcgatct gacggcggcg gcccggggct tgccgggctc ggcctgccgc agaagaactc
     5941 gacgcgtcgg ccgagggcga tttggccgac ctttggcttt cccgggttga atcggccgcg
     6001 ccgatttgaa accatgccgc ggggaaattt cgcgtcgtga gcagcccagg cgcggaaagg
     6061 aagggaggcg ctcatggcgg ccaagctgcg cgtcggcttt gtcggtctcg gccgcgtctt
     6121 cgatctcaac gtgcgcggct atctcggcca tcccgaggcg gaggtcgccg cgctttgcga
     6181 cagcgacacg gatttgctcg cccggcgcgc ggccgagcat cccaacgcgg tcgcgacccg
     6241 caatttcgag gaattcctgc gcgccgatct cgacatcgtc gagatcctga cgccgcaccc
     6301 gctgcacgaa gcgatggcgg tcgccgcgct cgagcgcggc gcccatatca gcgtgcaaaa
     6361 gccgatggcg atgacgctcc aggaatgcga ccgcatgatc gccgccgccg atcgaagcgg
     6421 cagaagactc aaggtcttcg agaacttcct gttctatccg ccgctcgtcc gcgcccgcca
     6481 attgctgcgc gacggcgcga tcggcaagcc gctgcatttc cgcatgaaag tggtgatggc
     6541 cgaccgcgcg caagcctgga ccgtgccgga aaatgcgagc cgctggcgcc atgcgctcgc
     6601 cgcggcggga cgcggcgggc cgatggtctt cgatcatggc catcacatga tggcggtggc
     6661 tctgtggctg ttcggcgacc tgcgcgaagg ctacgcctgc atcgagagca ccgagacccc
     6721 ggccggcgtt tacgacgccc cggcgacgct gacctggcgc catgccgatc ccccggtcca
     6781 cggcgtttgg gacgtttcgc tcgcgttgaa aatgacgctg cgcaccgact actacgccga
     6841 cgccgagcgc ttcgaaattc aaggcgaaga gggaatcctc caggtcacgc gctgctcgga
     6901 ccggatgttg gacgagcccg ttctcacgct ctaccgcgac ggcgaagtgc gggccttcca
     6961 caatctcgaa accgactggg ccgcaagctt tccgcgctcg acccggcact tcctcgacgt
     7021 gatcgccgga cgcgaagcgc agcaggtttt gacggggcgc gaaggccggc gggtgatcgc
     7081 gatgtacgac ctcttccagc gcagcaacgc ggagcggcgg gccctgacgg cgtgaggcga
     7141 gggccatgct cccgcttcag gcgatccgga caatccggaa tccttcgact tccttgacca
     7201 ccggcgagcc tttccagcgc tcgatgtcgt gcaacacgac aagccggctc aagtcgccgc
     7261 cgagttcgtc gttcatcttg tcgatcgtct tcaactgctc ccaaacgctg ccgaccgcgt
     7321 tgttgagcgg cacatagacg ccgtcatcgc catggccggt gaactgacgg cgcgaataga
     7381 cgcagtcgcc ggaaatcacc cggcggccgg ccgccgtttc gagaaggacg aattgctggc
     7441 caatcgtgtg gccgctgccg aggcgcacat gaatgcccgg cagaacgtcg ttcttgtcgc
     7501 cgtcgatcaa ggtgacgcgg tgagcgatcg aagcgtcgag cgccgtgcgc agattgtctg
     7561 gatcgataat cgccgtcagg tgaccgaagc gtttcggcag cgcaatggct tcgtaccagg
     7621 aaagcagctc gctcttttgg atatgaattt gcgcctttgg aaattcggcg atcgagccca
     7681 tatggtcgaa atgggcgtgc gtgatgaaaa tatcctcgac gtcgtcgggg ccgatgctca
     7741 tttcggccag catccggagc ggcgaaatcc aggtcgggat gccgaacttg cgggagaagc
     7801 tggagccgtg gctctcctgc atgaagccgg tgtcgatcag gacgttcttt ccaccgcagc
     7861 gcgccaggat gaacgagaag ggcaggtcca tcacgccctc cttgtacatg ccgctgatga
     7921 ggtcgaccca gggctgattt ttggagcggg cgaactccat gacatagact tcccattgcg
     7981 cggcttgcgg catgtttgag tctccgtcag ctctcggcga gcacgcggcc gagactcgaa
     8041 agcccgaccg ccacgatcag gatcgcgccc tggaagatgt attgagaaaa ggtcggcgcg
     8101 ccgaatatgt tcaaaccgtt gaatccaaag acgatgatta gggtcccaat gaaagtgccg
     8161 agcacgtgga actcgccgtc gcgcagcgtg gcggagccga gaaagacggc tgcgaaggcg
     8221 gtcaataggt acgagtcggc ggcgctggtc gtgccgctgc cgagccggga agcgagcagg
     8281 gcgccggtga gagcggcgca gacgccggat atgacgaagc ccaggatctt gatccggtcg
     8341 acgccgatgc cggcgagccg cgcggcgacc gggttggcgc ccgccgcctg gatttcctgt
     8401 ccgatcgggg ttcgctcgac caagacccac aacgcgccga ccaccagcgc catgacgatg
     8461 atcggattgg gcacgccgaa cagccaacgg ccaagcgcca attgcaggaa ggactccggc
     8521 acgccggcga cgatcgggac gccggcggaa taggcgaagg ataaaccgac aatgatcgtg
     8581 ccgacgccaa gcgtcgcgat aaccgagttc acgcggagct tggtgacgat gagtccgttg
     8641 acgacgccga tcgcgccgcc caaggcgaga acggccatga gcgcgaccgc gaccggcaac
     8701 ttggtctgca cgatcaaacc ggtgaccagg acgccatgca agctggccgc gtagccgatc
     8761 gaaagatcga gttcgccgac gatcacggcc agcgtcaagc cgcccgcgat gatcatcgcg
     8821 agcgaggcct gattgaggac attgatgaag ttgctatagg tcggaaaggc gtcgggcgat
     8881 aggatcgaga agacgagaat catcagcaga aggccgaaga tcgtcgcgaa gcgcgaaaag
     8941 acgctgagcc cgaccttcac ggccggcggc aatcgccggg tcgctacgga agtatcgctc
     9001 atgcggcggt ctgttgcggc gcttcggcgt agcttgccgc gatgatgcgc tcgcgcgtca
     9061 gcgattcgcc gatcagttcg gcgacaatgc gtccctccgc catgaccagg acgcggtcgc
     9121 acaggtccgg cagttcctcc ggctccgagg agacgacgag caccgccatg ccgcggctcg
     9181 cgagctcgcg caccagccgg tgaatctcgg cgcgggcgcc gacatcgacg ccccgcgtcg
     9241 gttcgtccag gatcagcgct ttcggcgccg cttgcagcca gcgaccgatg accaccttct
     9301 gctgattgcc gccgctgagc cgcccgaccg gcgcgtcgac atccgccgtc ctgatcgcga
     9361 ggtcgcggac gatcttcgcc gccatctgcg cgcgctttcg ggtgctgatg agcggcaggc
     9421 gtccaacgag gagaagcttc tggagattgg ccaggctgag gttgaaggcg acgctcttcg
     9481 acaggacgag gccctcgctg cgccgttcct ccggcacgaa gccgaggccc gctttcaccg
     9541 ccgccgcgca atcgcgcggg gcgaaaggct ggccgtccag cgtcgcgcgg ccgcgatcgg
     9601 gccggtcggc gccgaaaagg agccgcgcga gctcgctgcg tcccgcgccg accaagccgc
     9661 cgaggccgag cacctcgccg cgatgcaggt cgaaactgac gccgcgaact ttcggcggcc
     9721 gggcgaagcc gcgaacggcg agaacgacct tgccgcgcgg cggcgcagag agcggcgcct
     9781 tagcggtctc gaccgtgcgg ccgacgatcg cctcgacaag acccgtacgg gtcagcccgg
     9841 cgccatcgag ttcggccacc gactggccgt cgcgaaatac ggtgacgcgt tggcagagct
     9901 cgagga
//
LOCUS       DATGJW010000394         3241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_905294, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000394 DATGJW010000000
VERSION     DATGJW010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3241
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905294"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1082
                     /gene="sucA"
                     /locus_tag="VKU03_11910"
     CDS             <1..1082
                     /gene="sucA"
                     /locus_tag="VKU03_11910"
                     /EC_number="1.2.4.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386510.1"
                     /note="SucA; E1 component of the oxoglutarate
                     dehydrogenase complex which catalyzes the formation of
                     succinyl-CoA from 2-oxoglutarate; SucA catalyzes the
                     reaction of 2-oxoglutarate with dihydrolipoamide
                     succinyltransferase-lipoate to form dihydrolipoamide
                     succinyltransferase-succinyldihydrolipoate and carbon
                     dioxide; Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase E1 component"
                     /protein_id="HLJ72015.1"
                     /translation="SGEGIDWATGEALAFATLLDEGYPVRLSGQDSERGTFSQRHSVL
                     IDQETEARYIPLNHIRDGQARYEVINSMLSEEAVLGFEYGYSLAEPNALTLWEAQFGD
                     FANGAQVVFDQFISSGERKWLRMSALVCLLPHGYEGQGPEHSSARLERYLQLCAEDNM
                     QVANCTTPANYFHILRRQLKREIRKPLILMTPKSLLRHKRAVSSLSEIGPGSSFHRLL
                     WDDAESRKGDKIKLVKDDKIRRVILCSGKVYYDLYEDREKRGADDVYLLRVEQLYPFP
                     LKALVRELTRFKKAEILWCQEEPKNMGGWAFVEPYLEWVLAQVGGKSKRARYAGRPAS
                     AATATGLMSKHLAQMKAFLDECFAA"
     gene            1250..2470
                     /gene="odhB"
                     /locus_tag="VKU03_11915"
     CDS             1250..2470
                     /gene="odhB"
                     /locus_tag="VKU03_11915"
                     /EC_number="2.3.1.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045252 - oxoglutarate dehydrogenase
                     complex [Evidence IEA];
                     GO_function: GO:0004149 - dihydrolipoyllysine-residue
                     succinyltransferase activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoglutarate dehydrogenase complex
                     dihydrolipoyllysine-residue succinyltransferase"
                     /protein_id="HLJ72016.1"
                     /translation="MATEIRVPTLGESITEATVGKWFKQAGEAVKADEPLVELETDKV
                     TLEVNAPASGVLSEVAAKTGETVSVGALLGALAEGAGAAAKKNEAPAAAPAPAAAPAP
                     AAAMPPSPAAAKIAADNRIDLAGVAGSGKRGQVLKGDVLAAAQTPVALGTAPAAPPSP
                     TPVAARAPAAPDDASREERVRLTKLRQTIARRLKDAQNNAAMLTTFNEVDMTEVMALR
                     TKFKDAFEKKHGVKLGFMGLFVRACVQALKDVPAVNAEIDGADLVYKNYYHIGVAVGT
                     DKGLVVPVVREADRLGIAGIEKAIADFGRRARDGQLKIEEMQGGTFTISNGGIYGSLM
                     STPILNAPQSGILGMHKIQERPMAVNGKIEIRPMMYLALSYDHRVVDGKEAVTFLVRV
                     KEALEDPARLVLDL"
     gene            2603..>3241
                     /locus_tag="VKU03_11920"
     CDS             2603..>3241
                     /locus_tag="VKU03_11920"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019199379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLJ72017.1"
                     /translation="MAYDLIVIGAGPAGYVCAIRAAQLGMKVALVDKRKNGGGTCINI
                     GCIPSKALLYASEMYEEAKHGLGPLGIVVAPPKLDLAAMMKHKDDTVEANVKGLGFLF
                     RKNKIDSIVGSARLAAPGKVDVAGEDGATNSLEAKAIVIATGSDVARLPGIEIDEKRI
                     VSSTGALSLEKPPAKLLVVGAGVIGLELGSVWARLGAEVTVVEFLDRILPGMD"
BASE COUNT          608 a          995 c         1080 g          558 t
ORIGIN      
        1 aatccggcga ggggatcgac tgggcgacgg gcgaggcgct ggctttcgcg accctgctcg
       61 acgaaggcta tcccgtccgc ctttccggcc aggacagcga acgcggcacg ttctcgcagc
      121 gccattcggt gttgatcgac caggagaccg aggcgcgcta catcccgctc aaccatatcc
      181 gcgacggcca agcgcgctac gaagtcatca attcgatgct ctcggaagag gcggtgctcg
      241 gcttcgaata tggctattcg ctcgccgagc cgaacgcgct gacgctgtgg gaggcgcaat
      301 tcggcgattt cgccaacggc gcgcaagtcg tgttcgatca gttcatctcg tcgggcgagc
      361 gcaaatggct gcgcatgtcg gccctcgtct gcctgctgcc gcatggctat gagggccaag
      421 ggccggaaca ttcctcggcg cggctggagc gctatctgca gctttgcgcc gaagacaata
      481 tgcaagtcgc caattgcacg acgccggcga actacttcca catcttgcgc cgtcagttga
      541 agcgcgaaat ccgcaagccg ctgatcctga tgacgccgaa atcgctgctg cgccacaagc
      601 gcgcggtgtc gagcctgagc gagatcggcc ccggctcttc gtttcatcgc ttgctttggg
      661 acgacgccga atcgcgcaag ggcgacaaga tcaagctcgt caaggacgac aagatcaggc
      721 gcgtcatcct ctgctcgggc aaggtctatt acgatcttta tgaggatcgc gagaagcgcg
      781 gcgcggacga tgtgtatctc ctgcgcgtcg agcagctgta tcccttcccg ctcaaggcct
      841 tggtgcgaga gctgacgcgc ttcaagaagg ccgagatcct ctggtgccag gaagagccga
      901 agaatatggg cggctgggcg ttcgtcgagc cctatctcga atgggtgttg gcgcaagtcg
      961 gcggcaagtc caagcgggcg cgctatgccg gccggccagc ttcggccgcg acggcgaccg
     1021 gcttgatgtc aaagcatctc gcccagatga aggcgttcct cgacgaatgt ttcgcggcgt
     1081 gatcgacaat tgtcattgcg aggagcggag cgacaaagca atccgatcat agggcggcgt
     1141 cttacgatgg attgtttcgc ttcgctcgca ctgacggggc gtgatttagg ctttcaacca
     1201 tttgacctat gaccgatggg gatccgcttc gagcggagcg agtgacgaga tggcgactga
     1261 aatccgcgta ccgacgctcg gcgaatcgat caccgaggcg accgtcggca aatggttcaa
     1321 gcaggcgggc gaggcggtga aggccgacga gcccttggtc gagctcgaga ccgacaaggt
     1381 gacgctcgaa gtcaatgcgc ctgcgtcggg cgttctgtcg gaagtggcgg ccaagacggg
     1441 cgagacggtg tcggtcggcg cgctgctcgg cgcgctcgcg gaaggcgccg gcgccgctgc
     1501 gaagaaaaac gaagcgccgg cggccgcgcc cgcgcctgcc gcggcccctg cgccggcggc
     1561 cgcaatgccg ccctcgccgg cggccgccaa gattgccgcc gataaccgca tcgatcttgc
     1621 cggcgtcgcc ggatcgggca agcgcggcca agtgctgaaa ggcgacgtgc tggccgcggc
     1681 gcagacgcct gtcgcgctcg gcacggcgcc ggcggcgccg ccttcgccga cgcccgtcgc
     1741 ggcgcgcgcg cccgccgcgc ccgacgacgc ctcgcgcgaa gagcgcgtgc gcctgaccaa
     1801 gctgcgccag accatcgctc gaaggctgaa ggacgcgcag aacaatgccg ccatgctgac
     1861 gaccttcaac gaggtcgaca tgacggaggt catggctctc cgcacgaaat tcaaggacgc
     1921 attcgaaaag aagcacggcg tgaagctcgg cttcatgggc cttttcgtga gggcgtgcgt
     1981 ccaggcgctg aaagacgtgc ccgccgtcaa cgccgagatc gacggcgccg atctcgtcta
     2041 caagaattat taccacatcg gcgtcgccgt cggcacggat aaggggctcg tcgtgccggt
     2101 cgtgcgcgag gccgatcgcc tcggcattgc cgggatcgaa aaggcgatcg ccgatttcgg
     2161 ccgcagggcg cgcgacggac agcttaagat cgaggagatg cagggcggca ccttcaccat
     2221 ctccaatggc ggcatttacg gctcgctgat gtcgacgccg atcctcaatg cgccgcaatc
     2281 cggcattctc ggcatgcata agattcaaga acggccgatg gccgtcaacg gcaagatcga
     2341 aatccgaccg atgatgtacc tggcgctttc gtacgatcac cgcgtcgtcg acggcaaaga
     2401 ggcggtcacc ttcctggtgc gcgtcaagga ggcgctggag gacccggcgc ggctggtgct
     2461 ggatttgtga tcgcgtcgat gtcgttcccg cgaaagcggg aatccaggcg cctccgtgct
     2521 ttgaaggata ggcggtactg gattcccgct tgcgcgggaa tgacggagcg gctggagatt
     2581 tcaaagaatc gaggcgtctt gaatggctta tgacctcatc gtcatcgggg ccggccccgc
     2641 cggctatgtt tgcgcgattc gcgcggcgca gctcggcatg aaggtcgcgc ttgtcgacaa
     2701 gcgcaagaat ggcggcggca cctgcatcaa tatcggctgc atcccgtcga aggccttgct
     2761 ctacgccagc gaaatgtacg aagaggcgaa gcatgggctg ggcccactcg gaatcgtcgt
     2821 cgcgccgccg aagctcgatc tcgccgcgat gatgaaacac aaggacgata cggtcgaagc
     2881 caatgtcaaa ggtctcggct ttctgttcag gaagaacaag atcgattcca ttgtcggctc
     2941 ggctcgcctc gccgcgcccg gcaaggtcga cgtcgccggc gaggacggcg cgacgaattc
     3001 gctcgaagcc aaggcgatcg tcatcgccac cggatcggat gtggcgcggc tgcccggaat
     3061 cgagatcgac gagaagcgca tcgtctcctc gaccggcgcg ctgtcgctgg aaaagccgcc
     3121 ggcgaaattg ctcgtcgtcg gcgccggcgt gatcgggctc gaacttgggt cggtctgggc
     3181 gcggctcggc gcggaggtga cggtggtcga atttctcgac cgcatcctgc cgggcatgga
     3241 c
//
LOCUS       DATGJW010000395         2621 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1522694, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000395 DATGJW010000000
VERSION     DATGJW010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2621)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2621)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2621
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1522694"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1348
                     /locus_tag="VKU03_11925"
     CDS             <1..1348
                     /locus_tag="VKU03_11925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HLJ72018.1"
                     /translation="SAFAAAADTYRQLNLFGDVFEKIRTDYVEKPDDGKLVEAAINGM
                     LSSLDPHSSYMDAKAFREMQVQTKGEFGGLGIEVTQEDGLIKVVTPIDDTPAAKAGIL
                     SGDIITAIDGDPVQGMTLNQAVDKMRGAVNTPVTLKIQRGPKKEAMDIKIVRDIIKIQ
                     SVRSHIESGDIGYIRVTQFSEQTYDGLKAAMAKFHDQPGDANLKGYILDLRNNPGGLL
                     DQSIEVVNAFVDRGEIVSTRGRNVEETQRYNARPGADLSHGKPMVVLINGGSASASEI
                     VAGALQDHKRATLIGTRSFGKGSVQTIIPLGQNGALRLTTARYYTPSGRSIQAKGIEP
                     DIQILEDVPPDLKGKDDQKGEASLKGHLKNGTEEKDGSQAYVPPDPKNDKQLIAAVEL
                     LHGVKTAAQLASPPATPAPVSTPPAAAVTPSTPAPANAPVQPVKPPSPDSAPPAKP"
     gene            complement(1468..1920)
                     /locus_tag="VKU03_11930"
     CDS             complement(1468..1920)
                     /locus_tag="VKU03_11930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386203.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HLJ72019.1"
                     /translation="MLRDAVTAQMKEAMKGGDKTRVGALRLIMAALKDREIEARGAGA
                     ALSPADEMALLAKMVKTRQESLAIYEQAGRTDLASQERAEIAVINEFLPQQMDEAAVK
                     AAAEAAIAEIGAASIKDMGKVIGALKAKYPGQMDFAKASAIVKGLLGA"
     gene            2014..>2621
                     /locus_tag="VKU03_11935"
     CDS             2014..>2621
                     /locus_tag="VKU03_11935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbamoyl-phosphate synthase domain-containing
                     protein"
                     /protein_id="HLJ72020.1"
                     /translation="MTTLDSDGGGWTPPKATGLIVFADGAIVEGLGFGATGSAVGEIC
                     FNTAMTGYEEILTDPSYAGQIITFTFPHIGNVGVNDEDIETVNMAASAGARGVILRAE
                     ITDPSNFRATRRLDAWLRARGIVGVCGLDTRALTALIREKGMPNAVVAHAPDGAFDRA
                     ALAAEAKAWPGIAGMDLVPDVTAAQRYDWDETSWTLENGYGRR"
BASE COUNT          472 a          924 c          808 g          417 t
ORIGIN      
        1 ttcggctttc gctgcggcgg ccgacactta tcgccagctc aatctgttcg gcgacgtgtt
       61 tgagaaaatc cgtaccgact atgtagagaa gcccgacgat ggcaaactcg tcgaagccgc
      121 gatcaacggc atgctctcct cgctcgatcc gcattcgagc tatatggacg ccaaggcctt
      181 ccgcgaaatg caggtccaga ccaagggcga gttcggcggg ctcggcatcg aggtcacgca
      241 ggaagacggt ctgatcaagg tagtcacccc gatcgacgat acgccggcgg cgaaggccgg
      301 catcctttcc ggcgacatca tcaccgcgat cgacggcgac cccgtgcagg gcatgacgct
      361 caaccaggcg gtcgacaaga tgcgcggcgc ggtcaacacg ccggtgacgc tgaaaatcca
      421 acgcggcccc aaaaaggagg cgatggacat caagatcgtc cgcgacatca tcaagatcca
      481 gtcggtgcgc tcgcatatcg aaagcggcga tatcggctat atccgcgtaa cccagttcag
      541 cgagcagacc tatgacggcc tgaaagccgc catggccaag ttccacgacc agcccggcga
      601 cgccaatctc aagggctata tcctcgattt gcgcaacaat ccgggcggct tgctcgacca
      661 gtcgatcgaa gtggtcaacg cgttcgtcga ccgcggcgag atcgtctcga cgcgcggccg
      721 caacgtcgag gagacgcagc gctacaacgc ccggccgggc gccgacctct cgcatggcaa
      781 gccgatggtc gtgctgatca acggcggctc cgcctccgcc tcggagatcg tcgccggcgc
      841 gctccaggat cacaagcgcg cgacgctgat cggcacccgc tccttcggca agggctcggt
      901 gcagacgatc atcccgctcg gccagaacgg cgcgcttcgc ctgaccacgg cgcgctatta
      961 cacgccgtcc ggccgctcga ttcaggccaa aggcatcgag cccgacatcc aaatcctcga
     1021 agacgtgccg ccggatctga agggcaagga cgatcagaag ggcgaagcct cgctgaaagg
     1081 ccacttgaag aacggcacgg aggagaagga cggctcgcag gcctatgtgc cgccggaccc
     1141 gaagaacgac aagcagctca tcgcggcggt cgagcttctg catggcgtca agacagccgc
     1201 ccagctcgct tcgccgccgg cgaccccggc gccggtttcg acgccgccgg cggccgcggt
     1261 gacgccctcg acgcccgccc cggccaatgc gccggtgcaa ccagtcaagc cgccgtcgcc
     1321 cgattccgcg ccgccggcca agccgtaagg caaagcggat cagctacaaa acgaagcgcc
     1381 gccccttcgg gcggcgcttt tttcttgagc tgcctgcctt gatcgcgctg tagccgcggt
     1441 cgtcgaccgc ggctacaatc catcggctca cgcgccaagc agccccttca cgatggcgct
     1501 cgctttggcg aaatccatct ggccgggata tttcgccttc agcgcgccga tcaccttgcc
     1561 catgtccttg atgctcgccg cgccgatctc ggcgatggcg gcctcggccg cggctttgac
     1621 cgcggcctcg tccatctgct ggggcaggaa ttcgttgatc accgcgatct ccgcccgctc
     1681 ttggctggcg aggtcggtcc ggccggcctg ctcgtaaatc gccagcgatt cctggcgggt
     1741 cttcaccatt ttggcgagca gcgccatctc gtcggcgggg ctcagcgccg cgcccgcgcc
     1801 gcgcgcctcg atctcgcgat ccttcagggc ggccatgatc aggcgcagcg cgccgacgcg
     1861 cgtcttgtcg ccccccttca tcgcctcttt catctgcgcg gttacggcgt cgcggagcac
     1921 gggatcaaat ccttcatgga aacggcgccg cttcttgacg cggcggcggc ccgccgctta
     1981 agcacggggc gcatccaaat tcaacggctt ctcatgacca cgcttgacag cgacggcggc
     2041 ggctggacgc cgcccaaagc caccggcctc atcgtcttcg ccgacggagc catcgtcgaa
     2101 ggcttgggtt tcggcgcgac cggctcggcg gtcggcgaaa tctgcttcaa cacggcgatg
     2161 acgggctatg aggaaatcct caccgacccc tcctatgccg gccagatcat caccttcacc
     2221 ttcccgcata tcggcaatgt cggcgtcaac gacgaggata tcgagacggt gaacatggcc
     2281 gcctcggccg gcgcgcgcgg cgtgatcctg cgggcggaaa tcaccgatcc gtccaatttc
     2341 cgcgccacgc gccggctcga cgcctggctg agagcgcgcg gcattgtcgg cgtctgcggc
     2401 ctcgacaccc gcgccttgac cgcgctgatc cgcgaaaagg gcatgcccaa cgcggtcgtc
     2461 gcccatgcgc ctgacggcgc cttcgaccgc gccgcgctcg ccgccgaggc gaaagcctgg
     2521 ccgggcatcg ccggcatgga cctggtgccg gacgtcaccg ccgcccagcg ctacgactgg
     2581 gacgagacga gctggacgct cgaaaacggc tatggccggc g
//
LOCUS       DATGJW010000396         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_485299, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000396 DATGJW010000000
VERSION     DATGJW010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_485299"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..883)
                     /locus_tag="VKU03_11940"
     CDS             complement(<1..883)
                     /locus_tag="VKU03_11940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitase family protein"
                     /protein_id="HLJ72021.1"
                     /translation="MTSLDSFKCRKTLKVGAKTYEYYSLKAAEKNGLAGVSQLPFSMK
                     VVLENLLRYEDGRTVKKSDIEAVAAWLDNRGKKEHEIAFRPARVLMQDFTGVPAVVDL
                     AAMRNAMEALGGDPNKINPLVPVDLIIDHSVVVDYFGNSKALAQNVKREYEQNQERYR
                     FLKWGQQAFDNFSVVPPGTGICHQVNLEYLAQTVWTRKEKAGKASVEIAYPDTLVGTD
                     SHTTMVNGLSVLGWGVGGIEAEAAMLGQPLSMLLPEVVGFKLTGELREGVTATDLVLT
                     VTQMLRKKGVVGKFVEFY"
     gene            1006..1635
                     /gene="ccmA"
                     /locus_tag="VKU03_11945"
     CDS             1006..1635
                     /gene="ccmA"
                     /locus_tag="VKU03_11945"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612331.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HLJ72022.1"
                     /translation="MRLRADHIAIERSGRRLFSELSFELQSGQALAVRGPNGAGKTSL
                     LRAVAGLLPLVEGALSLEGGDEEAPLGEQTHYLGHADALKGALSARENLAFWAAMLAP
                     AGTLIGGKAPMSVPAALDRVGLSHAIDLPVQALSAGQKRRVALARLLLAWRPLWLLDE
                     PLTALDQAAQQRLHEILREHLAAGGLIVAATHAPLDLETSRELRLEAAP"
     gene            1632..2303
                     /gene="ccmB"
                     /locus_tag="VKU03_11950"
     CDS             1632..2303
                     /gene="ccmB"
                     /locus_tag="VKU03_11950"
                     /EC_number="7.6.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HLJ72023.1"
                     /translation="MRKAAAALFLREWRIARRIGGGAAIGLVFFLILVTLIPFAVGPD
                     QRLLGRIGPAVLWIAGLLATLLGVDRLFQSDDEDGSLDLLVHGRLPLELVVLVKCAAH
                     WLASALPLVAASPLFGLMLALDGKALGLVAASLAVGTPALTLIGAIGASLTVSLRRGG
                     LLLAIIALPLTIPVLIFGVAAASAASGGAAPFAPPFLALCALTLAAIAGAPFAAAWAL
                     RLARG"
BASE COUNT          374 a          824 c          801 g          476 t
ORIGIN      
        1 cgtagaactc gacgaacttg ccgaccacgc ctttcttgcg cagcatctgg gtcacggtga
       61 ggacgagatc ggtcgcggtg acgccctcgc gcaattcgcc ggtcagcttg aagccgacga
      121 cttccggcag gagcatggag agcggctgtc caagcatcgc cgcctccgcc tcgatgccgc
      181 cgacgcccca gccgagaacg gaaagaccat tgaccatggt cgtatggctg tcggtgccga
      241 ccagcgtgtc gggataggcg atttcgacgc tcgccttgcc cgccttctcc ttgcgcgtcc
      301 acaccgtctg ggcgagatat tcgagattga cctgatggca aatgccggtg ccgggcggca
      361 ccacggagaa attgtcgaac gcctgctgac cccatttcag gaagcgatag cgctcctggt
      421 tctgctcgta ttcgcgcttg acgttctgag ccagggcttt gctgttgccg aaatagtcga
      481 ccacgaccga atggtcgatg atgaggtcga ccggcacgag aggattgatc ttgttcggat
      541 cgccgcccaa cgcctccatg gcgttgcgca tcgcggcgag atcgacgacc gccggtacgc
      601 cggtgaaatc ctgcatcagc acgcgggccg gacgaaacgc gatctcgtgc tctttcttgc
      661 cgcgattgtc gagccaggcg gcgaccgctt cgatgtcgct tttcttgacc gtgcgcccgt
      721 cttcgtagcg cagaagattt tcaagaacga ccttcatcga aaagggcaat tgcgaaacgc
      781 cggcgaggcc gttcttctcg gcggccttca aagaatagta ttcataagtc ttggcgccga
      841 ctttcaacgt cttgcggcat ttgaaactgt cgagcgaggt catcgacatc gtctccggtc
      901 acgatttttg ggcaatcggc aggccttata gataagttcc ccagggcagg ctacagaaac
      961 aatcgtcgcg gagcgcaagg ctaggctgcg caaaaaggag aggggatgcg gcttcgcgcc
     1021 gatcatatcg ccatcgagcg cagcggccgc cggctttttt ccgaactctc tttcgagctt
     1081 caatcgggac aggctttggc ggtgcggggg ccgaacggcg ccggcaagac gagcttgctg
     1141 cgcgcggttg ccgggcttct gccgctggtc gaaggcgcgc tctccctcga aggcggcgac
     1201 gaggaggcgc cgctcggcga gcagacgcac tatctcggcc atgccgacgc gctgaaaggc
     1261 gcgctaagcg cgcgggagaa tctcgccttc tgggcggcga tgctcgcccc cgccggaacg
     1321 ctcatcgggg ggaaagcgcc gatgagcgtt ccggcggcgc tcgatcgcgt tggcctcagc
     1381 catgcgatcg atttgccggt tcaggcgctc tcggcaggtc aaaagcggcg tgtcgctctg
     1441 gcgcggctgc tgctcgcctg gcgcccgctt tggcttctcg acgagccgct gacggcgctc
     1501 gatcaagccg cgcaacagcg gttgcatgaa atccttcgcg agcatctcgc cgccggcggg
     1561 ctgatcgtcg ccgccaccca tgcgccgctc gatctcgaga cctcgcgcga attgcggctc
     1621 gaggccgcgc catgaggaaa gccgccgccg cgctcttcct gcgcgaatgg cgcatcgccc
     1681 ggcgcattgg cggcggcgcg gcgatcggcc tggttttctt tctgatcctg gtgacgctga
     1741 ttcccttcgc cgtcggtccg gatcagcgcc tgcttggccg gatcggaccg gcggtcctat
     1801 ggatcgccgg tctgctcgcg accctgctcg gcgtcgaccg gctattccag agcgacgacg
     1861 aggacggctc gctcgatctg ctcgttcatg gccgcttgcc gctcgaactc gtcgttctcg
     1921 tcaaatgcgc agcccattgg ctggcgagcg ccctgcctct cgtcgccgcc agcccgcttt
     1981 tcggcttgat gctggcgctc gacggcaagg cgctcggcct ggtcgcggcg agcctcgccg
     2041 tcggcacgcc ggctctgacg ctgatcggcg cgatcggcgc gtcgctcacc gtctcgctcc
     2101 gccgcggcgg cctgctcctg gcgatcatcg cgctgccgct gaccattccg gttctgattt
     2161 tcggcgtcgc cgccgcctcg gcggcgagcg gcggcgcggc gccgttcgcg ccgcccttcc
     2221 tggcgctctg cgccttgacc ctcgccgcga tcgccggcgc gcccttcgcc gcggcctggg
     2281 ccttgcgcct ggctcgaggc tgagcgccgc gacgccgact tgagttcgca atcgtccggc
     2341 gcttaattgg cggcggcctc ggagcgggcg gagattttca tggcgggatt cgacgacgat
     2401 ccctttggcg cgcgcaaggc gccgacagcg catgagatcg gccagacgat cgacacgctc
     2461 tccgtgcacg agctc
//
LOCUS       DATGJW010000397         4952 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1213198, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000397 DATGJW010000000
VERSION     DATGJW010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4952)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4952)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4952
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1213198"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..745)
                     /locus_tag="VKU03_11955"
     CDS             complement(<1..745)
                     /locus_tag="VKU03_11955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012319323.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016881 - acid-amino acid ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mur ligase domain-containing protein"
                     /protein_id="HLJ72024.1"
                     /translation="MKLPRELGPIHFIGIGGIGMSGIAEVLLNLGYKVQGSDASDNAN
                     AKRLREKGARVFVGHDAAHLGEAAVVVVSTAIKRDNPELAAAREKRLPVVRRAEMLAE
                     LMRLKQCVAIAGTHGKTTTTSLVATLLDAAGLDPTVINGGIINAYGTNARLGAGDWMV
                     VEADESDGTFLKLPADIAIVTNVDPEHLDHFKTFDAVQDAFRAFVENIPFYGFAVMCT
                     DHPVVQTLVGKLEDRRVLTYGLNPQADVLL"
     gene            complement(852..1247)
                     /locus_tag="VKU03_11960"
     CDS             complement(852..1247)
                     /locus_tag="VKU03_11960"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ72025.1"
                     /translation="MIGLDTNVLLRVFIEDDPAQSERARRLVRRATAEGRVLINSIVL
                     SEFAWTLARTFKLGRLEIARLLAELLSADDLEFERREAAKAALDAYRAGEADFPDYYL
                     AEVNAGLGCASTATFDRAALKHSRFSSVP"
     gene            complement(1251..1547)
                     /locus_tag="VKU03_11965"
     CDS             complement(1251..1547)
                     /locus_tag="VKU03_11965"
                     /inference="COORDINATES: protein motif:HMM:NF027267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0097351 - toxin sequestering activity
                     [Evidence IEA];
                     GO_process: GO:0001558 - regulation of cell growth
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system PrlF family
                     antitoxin"
                     /protein_id="HLJ72026.1"
                     /translation="MLKLGSEIAEATVTSKGQITVPSEIRKLLHLAAGDRLRFFQSKN
                     GSVVIEARKRRSILDIARENPLKAPKPLGDLDAEINAAVGEAMAERERRARAKS"
     gene            complement(1586..1993)
                     /locus_tag="VKU03_11970"
     CDS             complement(1586..1993)
                     /locus_tag="VKU03_11970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003544794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ72027.1"
                     /translation="MIVIERLDHLVLTVADIERACAFYAAVLGLTVETFGEGRKALRF
                     GDQKINLHRAGHEFEPKAKKPTPGSGDLCFIAETSMAEVIEHLRRCGVAIEVGPVERE
                     GAAGALVSVYIRDPDGNLIEIANRDLSLRPAFP"
     gene            complement(1990..3381)
                     /locus_tag="VKU03_11975"
     CDS             complement(1990..3381)
                     /locus_tag="VKU03_11975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173586.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase family protein"
                     /protein_id="HLJ72028.1"
                     /translation="MSLSFAAPTPNAVPIHFVTKDNWDDLRAALPTPAAAFAASSGFE
                     PKPGRCLTLPSASGEICAALFGLESAEGPCDLLLPGKLATSLPAGLFRFANQPHDASL
                     AALAFLLASYRFGRYRAADKKQPSLIAPEGVDGGRLERMAAAIAFGRDLVNTPANDLG
                     PAALEREAVGLAESFAAEVEVTRGDDLLKRNLPLIHAVGRAAGEAPRLVDFAHGRFGA
                     PKVTLVGKGVTFDTGGLDIKPASAMELMKKDMGGAAAALALARMIMAAKLDLRLRVLI
                     PIVENAISATAFRPGDVLKSRKGLTVEIGNTDAEGRLILADALALADEDEPALMFDFA
                     TLTGAARVALGPDLPPFYTDDETLAAEIARHARETADPVWRLPLWPPYESMLDGKIAD
                     LNNAPAGPFAGSITAALFLKRFVAKTAAWAHFDVYAWNPKPRPYGPEGGEVQAARALF
                     ALLEERFKAEAAR"
     gene            3518..4393
                     /locus_tag="VKU03_11980"
     CDS             3518..4393
                     /locus_tag="VKU03_11980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ72029.1"
                     /translation="MASIMKGRSAALALFFGLAAPLGGCQGMDDVTGSVASASVSTST
                     ALPTSDEELRAYAESWGKRYDANPGEKVASINYARALRALTRYQQAVAVMESAAVKAP
                     QDFDILAAYGKALADNGQLAQAADVLSRSYTPDRPNWASMSAQGSVADRLGDHEQAQD
                     LYLRALKIAPNEPTVLTNLGLSYALTKQLPLAEQTLRQAAAQPGADRRVRDNLALVLA
                     LEGKFAEAQKVNERDMSPQAAAANVAAVRQMIAQSNSWREIQTLDAKKRDHTPTKAKS
                     DAAAPAAPTQLQPAG"
     gene            4585..>4952
                     /locus_tag="VKU03_11985"
     CDS             4585..>4952
                     /locus_tag="VKU03_11985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TSUP family transporter"
                     /protein_id="HLJ72030.1"
                     /translation="MLHLLPFSTLAALALIGLVAGFVDAIAGGGGLLTVPALALAGLD
                     PVSAVATNKVNGVCGTASAAWTFARLGKIDLAAMWPSAVTAGLGAIAGALVLPIAPRG
                     AVDAALPLVLIAVALYFAFSP"
BASE COUNT          859 a         1639 c         1621 g          833 t
ORIGIN      
        1 tcagcaggac gtccgcttgc gggttgaggc cgtaagtgag gacacgacgg tcttccaatt
       61 tgccgaccaa tgtctgcacg accggatggt cggtgcacat caccgcgaat ccgtagaacg
      121 ggatgttctc gacgaaggcc cgaaaagcgt cctgcaccgc atcgaaagtt ttgaaatgat
      181 cgagatgttc cggatcgaca ttggtcacga tggcgatgtc ggcgggcaat ttgagaaatg
      241 tgccgtcgga ttcgtccgct tcgaccacca tccaatcgcc ggcgccgagc cgggcgttcg
      301 tcccgtaggc gtttatgatg ccgccattga tcacggtcgg atcgagaccg gcggcgtcga
      361 gcaaagtcgc gaccagcgag gttgtcgtcg tcttgccatg cgtgccggcg atcgcgacgc
      421 attgcttgag ccgcatcagc tcggcgagca tttcggcgcg gcgcaccacc ggcaggcgct
      481 tttcgcgcgc cgcggcgagc tcgggattgt cacgcttgat cgcggtcgag acgacgacga
      541 ccgccgcctc gccgagatgg gccgcgtcat ggccgacaaa gacccttgcc cccttctcgc
      601 gcaggcgctt ggcgttagcg ttgtcgctgg cgtccgagcc ctgcacctta tagccgaggt
      661 tgagcaggac ctcagcgatg ccggacatgc cgatgccgcc gatgccgatg aaatggatgg
      721 ggccgagctc gcgcggcagc ttcatcgaaa tggaccttct tggcggggcc aagccgcccc
      781 gccctctttg cgccggcata aggcgaatcg gcgattcgga caaatcggca agcggcgcga
      841 acgccgcgag cttatggtac cgaggaaaaa cgagaatgtt tgagcgcggc gcgatcgaaa
      901 gtggccgtcg aagcgcagcc aagcccggcg ttgacctcgg caaggtaata gtccgggaaa
      961 tcggcctcgc ctgcgcgata ggcgtcaagc gccgccttcg cagcctcacg gcgctcgaat
     1021 tcgagatcgt cggccgacag gagctccgcg agcagacgcg caatttcaag cctcccgagc
     1081 ttgaaagttc gcgctagagt ccaggcaaat tccgacagga cgatcgaatt gataagaacg
     1141 cgcccttcgg ccgtcgcgcg ccgaacgagc cggcgcgcac gttccgattg agcgggatcg
     1201 tcctcgataa aaacccgcag gaggacgttg gtgtcaaggc cgatcatcgg ctaagatttt
     1261 gcgcgagcgc gacgctcccg ctccgccatc gcctcgccca ccgcggcgtt gatttcggcg
     1321 tcgagatctc caagcggttt gggcgccttc agaggatttt cccgcgcaat atcgaggatg
     1381 ctgcggcgtt tgcgcgcctc gatgaccacg gagccgttct tcgattgaaa gaagcgcagc
     1441 cggtctccgg ccgccaaatg caggagctta cgaatttcgc tggggacggt gatctgtccc
     1501 ttgctggtca cggtcgcctc ggcaatttcc gacccgagct tgagcataac gcgcgctcct
     1561 tacagcttta ttatccttac ataggtcatg gaaaggcggg acgcaaggaa aggtcccgat
     1621 tggcgatttc gatcagattc ccgtccgggt cgcggatata gaccgagacc agcgcgcccg
     1681 ccgcgccctc gcgctcgacc ggcccgactt cgatcgcgac gccgcagcga cggagatgct
     1741 cgatcacctc cgccatcgaa gtttcggcga tgaaacagag gtcgccggag ccgggcgtcg
     1801 gcttcttggc cttgggctcg aactcatggc cggcgcggtg aaggttgatc ttctggtcgc
     1861 cgaagcgcag cgccttgcgc ccctcgccga acgtctcgac cgtcaggccg agcacggccg
     1921 catagaaggc gcaagcgcgc tcgatgtcgg cgactgtcag aacgagatgg tcgaggcgct
     1981 cgatgacgat caccgcgccg cctccgcttt gaaccgctcc tccagcaggg caaagagcgc
     2041 ccgcgccgcc tgtacttccc cgccctccgg accgtatggc cgcggcttcg gattccaggc
     2101 gtaaacgtcg aaatgcgccc acgccgcggt cttggcgacg aagcgcttga ggaacagagc
     2161 cgccgtgatc gagcctgcga aggggccggc gggggcgttg ttgagatcgg cgatcttgcc
     2221 gtcgagcatc gattcgtaag gcggccatag cggcaagcgc caaaccggat cggccgtctc
     2281 tctcgcatgg cgcgcgatct cggcggcgag cgtctcgtcg tccgtgtaga acggcggcag
     2341 atccggcccg agcgccaccc gcgccgcgcc ggtcagcgtg gcgaagtcga acatcagcgc
     2401 cggctcgtcc tcgtccgcca aagcgagcgc gtcggcgagg atgaggcggc cctcggcatc
     2461 ggtattgccg atctcgacgg tcaggccctt gcggctcttg agcacgtcgc cggggcgaaa
     2521 ggccgtcgcg gaaatcgcgt tttcaacgat cgggatcagc acgcgcagac gaagatcgag
     2581 cttggccgcc atgatcatgc gggccagcgc caaggccgca gccgcgccgc ccatatcctt
     2641 tttcatcagc tccatagcgc tggccggctt gatgtcgagc ccgccggtgt cgaaggtcac
     2701 gcccttgccg accagcgtca ctttcggcgc gccaaaccgg ccatgcgcaa aatcgacgag
     2761 ccgcggcgcc tcgcccgccg cgcggccgac cgcatggatc agcggcagat tgcgcttcag
     2821 gagatcgtcg ccgcgcgtca cctcgacttc agccgcaaag ctttccgcga ggccgacggc
     2881 ctcccgctcc agagccgcgg ggccgagatc gttggccggc gtgttgacga gatcgcggcc
     2941 gaaggcgatc gcggccgcca tccgctccag cctcccgccg tcgaccccct ccggcgcgat
     3001 caggctcggc tgcttcttgt ccgcggcgcg ataacgcccg aaccgatagg aggcgagcag
     3061 aaaggcgagc gcggcaaggc tcgcgtcatg cggctgattg gcgaagcgaa agaggcccgc
     3121 cggcagcgac gttgcgagct tgcccggcag caagaggtcg cacggccctt ccgcgctttc
     3181 gaggccgaac aaagcggcgc aaatctcgcc gctggcgctg ggcagcgtca ggcagcggcc
     3241 gggcttcggc tcgaagccgg aagaagcggc gaaggccgcc gccggcgtcg gcagggcggc
     3301 gcgaaggtcg tcccaattgt cttttgtcac aaaatggatc ggcacagcat tcggcgtcgg
     3361 cgcggcgaag gacagggaca ctctgggctc catggagggg acggaggata gggcttggcg
     3421 gcgcgccggc aaattcccgg aagaccgcct cgttaacaca tcgttagcct taacaaatta
     3481 ttgctgccct ggatgggccg cgacggggga cggcaagatg gcttcgatca tgaagggccg
     3541 gagcgcggct ttggcgctgt ttttcggcct cgccgcgccg ctcggcggct gccaaggcat
     3601 ggacgacgtg accggctccg tcgcttcggc ttcggtttcg acctcgaccg cgctgccgac
     3661 cagcgacgag gagctgcgcg cctatgccga gagctggggc aagcgctacg atgcgaatcc
     3721 cggcgagaag gtcgcctcga tcaattacgc tcgcgccttg cgcgccctga cccgctatca
     3781 gcaggccgtg gcggtgatgg aatcggccgc ggtgaaagcg ccgcaggatt tcgacatcct
     3841 cgccgcttat ggcaaggcgc tcgccgataa tggccaattg gcgcaggccg cggatgtcct
     3901 gtcgcgctcc tatacgccgg atcgccccaa ttgggcgagc atgtcggcgc agggctcggt
     3961 cgccgaccgg ctcggcgatc acgagcaggc gcaagacctt tatctccgcg cgcttaaaat
     4021 cgcgccgaac gaaccaaccg tcctcaccaa tctcggcctc tcctatgctt tgaccaagca
     4081 attgccgctg gccgagcaga ccttgcggca agcggcggcg cagcctggcg cggaccgccg
     4141 cgtgcgcgac aatctcgcgc ttgtcctggc gctggaaggc aaattcgccg aagcgcagaa
     4201 ggtcaacgag cgggacatgt cgccgcaagc ggccgccgcc aatgtcgcgg ccgtgcgcca
     4261 gatgatcgcg caatccaatt cctggcgcga aatccagacg ctcgacgcca agaagcgcga
     4321 tcacacgcct accaaagcaa agagcgacgc cgccgccccc gctgcgccga cgcagcttca
     4381 gccggcggga tgattccatc ctcccccgtc gcaaagcggg ggagccacgc gaagcatggt
     4441 ggagggggag tctatcaaat ataacgcgca agcgccgcag gtccctccac cgcgctgacg
     4501 cacggtcccc ctgccccgtt tcgcttcgct tcaccgggag gatagaggcg cggcgctccg
     4561 cactttccga caaagcgagg ccgcgtgctg cacctcttgc cgttctcgac gctcgccgcc
     4621 ttggcgctga tcggcctggt cgccggcttt gtcgacgcca tagcgggcgg cggcggcttg
     4681 ctgacagtgc cggctttggc cttggcgggg ctcgatccgg tttcggcggt ggcgaccaac
     4741 aaggtcaatg gcgtgtgcgg caccgcgtcg gcggcttgga ccttcgcccg gcttggcaag
     4801 atcgacctcg ccgcgatgtg gccctcggcc gtgaccgccg ggctcggcgc gatcgccggc
     4861 gcgctggtcc tgccgatcgc cccgcgcggc gcggtcgatg cggccctgcc tttggtcctc
     4921 atcgccgtcg cgctctattt tgccttttcg cc
//
LOCUS       DATGJW010000398         8124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1523077, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000398 DATGJW010000000
VERSION     DATGJW010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8124
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1523077"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(281..3088)
                     /gene="ppc"
                     /locus_tag="VKU03_11990"
     CDS             complement(281..3088)
                     /gene="ppc"
                     /locus_tag="VKU03_11990"
                     /EC_number="4.1.1.31"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891269.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008964 - phosphoenolpyruvate carboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0015977 - carbon fixation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxylase"
                     /protein_id="HLJ72031.1"
                     /translation="MGDLVSIAALGEAAFPAAAPPAPDSPDKDSPLREDIRLLGRILG
                     DTVRQQEGGALFDIVERIRQASIRFHRDGDMSARRELEAILDSLTADQTLIIVRAYSY
                     FSHLANIAEDQHHTRRNRAHVVAGSSPRAGTLAHALKRARQAGIGDEALRAFFNGALM
                     SPVLTAHPTEVRRKSMLTREIEIGEIIDQRERMAGNAPELAESEERLRRAVLTLWRTS
                     MLRQTRLKVLDEVANGLSYYDYTFFAELPRLYAALEDELSCGGKSENQPLASFLRVGS
                     WIGGDRDGNPFVTADVLRETTRLQSATAIRRHLDELHELGGELSLSAVLTPITPALAE
                     LAERSTDRSMTRADEPYRRAIGGVYSRLAATARELDQVVALRRPIAEAPAYASAKELA
                     ADLEIIARSLEENGAAILARGRLRRLRRAVDCFGFHLAPVDLRQNSDVHERTVAELFA
                     KAAPGVDYLALNEKGRVALLLKELANPRPLVSPFLAYSEETQGELAVFKTAAEIRRVY
                     GEEAIRTSIISKAQGVSDMLELALLLKEVGLVSADGRSALHLVPLFETIDDLRHCAGV
                     MDELLAIPEYRSLVDSRGGEQEVMLGYSDSNKDGGFVTSGWELYKAEIGLVDVFRRHG
                     VRIRLFHGRGGSVGRGGGPSYDAILAQPGGAVQGQIRITEQGEIISSKYSNPEVGRRN
                     LEILAAATLEASLLEEEVAAPDPAYLSAMEQLSESAYKAYRGLVYETEGFERYFWSST
                     VITEIATLNIGSRPASRKKTTRIEDLRAIPWVFSWAQCRLMLPGWYGFGAAVKEFVAA
                     HPDGLKQLQDMHRQWPFFRTLLSNMDMVLAKSSLAIASRYAQLVPDVALREAIFSRIS
                     REWRDSVDALLAIMGHKKLLEANPLLDRSIRNRFPYLDPLNHVQVELLKLHRSQPGNE
                     KVLTGIQLTINGVSAGLRNSG"
     gene            3280..3675
                     /locus_tag="VKU03_11995"
     CDS             3280..3675
                     /locus_tag="VKU03_11995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72032.1"
                     /translation="MRSVTAGLARQVVVAVAMSALLIAGLSVRPSGGARPPANAAAEK
                     PIATRAIVTAEAPPAAPVKRAAAKLAAARLRLVAPTPRPRPADPAAKVIAAAYVPPGP
                     EPSAPSLKKRLFAPIGFVRDNVARLISWL"
     gene            3758..4195
                     /locus_tag="VKU03_12000"
     CDS             3758..4195
                     /locus_tag="VKU03_12000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013206063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF29 domain-containing protein"
                     /protein_id="HLJ72033.1"
                     /translation="MSASYEKDVLLWSEEQARLLRTGQFGRLDIEHLADEIEDVGRSE
                     ERELASRMAVLLARLLKWRFRPEMRTPRWRKTINHQRQRVELILKATPSLKTAMSDAD
                     WQKGAWLDAIGQAAQETGLDEAVFPETCPWEMRATLDAAFWPD"
     gene            4286..5806
                     /locus_tag="VKU03_12005"
     CDS             4286..5806
                     /locus_tag="VKU03_12005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72034.1"
                     /translation="MSATAAGRSAAASAPSERRLRTGLRAERLIPAGAALAALAIGLW
                     RPANTDISWLLTVNERILDGATPYKDVIELNPPASILLYRLPVLAAHWLSLRAEIAVV
                     AFIALLIGGVLIYARRLLSRYELCNPARRAVFLLVAALVLAMLPFDEMAQREHFATIF
                     ALPYALVAMARAAERRAAPLDAIIAGAMMGLCVAVKPHFALCALLVAAAEAWRARAAG
                     AFFRIENLAAFAVALAYVIGSFLFFPYFFSDVLPMVRDLYLPIRLGAAGLIAHIAPIL
                     ALPLCLCWLFRAEPQAGGARILLLIAAGFLAAYFLQGKGWSYHAYPALAFSLLAAAWA
                     LPQAADAVARLSPKLGALLLAAALIAPAPSFFRRDERHAALAAAIAKIAPHPKLLALS
                     FRQSLGHPLTRNIGGVWVGRYWGLWPTGLAVLMKERAGDDATLRAKADAYFEKDRLAA
                     AEDIEHQKPDIVLIDETPGFDFNAWIAASPQLEAALARYKPMQAIDGVEILQRRNS"
     gene            complement(5904..7085)
                     /locus_tag="VKU03_12010"
     CDS             complement(5904..7085)
                     /locus_tag="VKU03_12010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008550601.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLJ72035.1"
                     /translation="MSETFDVVIGGGAVMGSSIAYHLAAHPGFSGRVLVVDKDMTYQR
                     SASALSLSSIRQQFSTAVNIRVGLYGVAFLRKAKETLAADGVAPELSFRENGYLYLAS
                     GQGAGILAENHVVQSAEGADILLLEPPSLAERFAFLNLDGVALGAWGRRNEGWFDGYT
                     LMQAFRRKAKSLGAEYREAVIIGFERDGARVVAARLSTGERIACGAFVNAAGASGAAQ
                     LADMLGVAIPVRRRKRSVFVFAAKERLAHCPLIIDTSGVYVRPEGENYVCGVSPPEED
                     DPDSDDFDVVWRHFDDIIWPALAARIPAFEAIRPGRAWACHYDLNIFDHNAIVGRLGG
                     LENAYLAAGFSGHGMQQSPAIGRGLAELIVEGRYATLDLSPFAFERIAANRPLLERNV
                     I"
     gene            7326..8108
                     /locus_tag="VKU03_12015"
     CDS             7326..8108
                     /locus_tag="VKU03_12015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72036.1"
                     /translation="MTRLAGKAAIVTGAAHGIGLACARRLAADGAAVALADIDAKAGE
                     EAARALRDQGARAIFVATDVAQRPALAALIDRAASEFGRLDIMLNNAGVALSAPIVEM
                     SDELFDKVLSINLRAAFVGTQLAARKMIAAKNGGVIINMSSVNALLAIPGLAAYACTK
                     GALNQLTKVAAVELAPHKIRVVAIGPGTILTELAKKAVLSDEAARKKILSRTPIGRAG
                     EPEEVAAVASFLASADASYITGQTIYPDGGRLALNYTVPAPD"
BASE COUNT         1362 a         2679 c         2719 g         1364 t
ORIGIN      
        1 cgccggcgcc gtaacgagcg ttcgcaaaac ggtcatagtc cgacatcgaa gacctcttca
       61 atcgtgtctt gcgcgtcccg ccggctggtc ggacggcgcc gcgctcgcgc tatcatatgg
      121 ccccgccccc tccccgcgca agggagccaa gaacgaaggg cgcaaggact ctgttttatt
      181 acaaatattt aacccaggcg aggcgaatgg cgaatagcga atagggatga tggcggaacc
      241 gccgccctat tcgcctcgcc attcgccatt cgccattcgc tcatccgcta ttcctcaatc
      301 ccgccgaaac gccgttgatc gtcagttgaa tgccggtcag caccttctcg ttgccgggct
      361 ggctgcgatg cagcttgagc agctcgacct gcacatggtt caagggatcg agatagggga
      421 agcggttgcg gatcgagcgg tcgagcagcg gattggcttc gagcagcttc ttgtggccca
      481 tgatcgcgag cagcgcgtcg accgaatcgc gccattcgcg gctgatgcgc gagaagatcg
      541 cctccctgag cgcgacatcg ggcacgagtt gggcatagcg cgaggcgatg gcgagactgc
      601 ttttggcgag caccatgtcc atattggata agagggtgcg gaagaacggc cattgccggt
      661 gcatgtcctg aagctgcttc aatccgtccg gatgggcggc gacgaattcc ttcaccgccg
      721 cgccgaagcc gtaccagccc ggcagcatca gccggcattg cgcccaggaa aagacccagg
      781 gaatggcgcg cagatcctcg atccgcgtcg tctttttgcg cgaggccggg cgcgagccga
      841 tgttcagcgt cgcaatctcg gtgatcacgg tcgaagacca gaaatagcgc tcgaagcctt
      901 ccgtttcgta gaccaggccg cgataggcct tgtaggcgct ctcggagagc tgctccatcg
      961 ccgaaagata ggccgggtcg ggcgccgcga cctcttcttc cagaaggctt gcttcgagcg
     1021 tcgccgcggc gagaatttcg agattgcgcc gcccgacttc cgggttcgag tatttggagg
     1081 aaatgatctc gccttgctcg gtgatgcgga tctggccctg aaccgcgccg cccggctggg
     1141 cgaggatcgc gtcgtagctc ggcccgccgc cgcggccgac cgagccgccg cggccatgga
     1201 acaagcggat gcgcacgcca tggcgcctga acacgtcgac caggccgatc tccgccttgt
     1261 aaagctccca gcccgacgtg acgaagccgc cgtccttgtt cgagtccgaa tagccgagca
     1321 tgacttcctg ctcgccgccg cggctgtcga cgaggcttcg gtattcgggg atggcgagaa
     1381 gctcgtccat cacgccggcg caatggcgca gatcgtcgat cgtctcgaac agcggaacca
     1441 gatgcagcgc gctcctgccg tcggcgctga caaggccgac ttctttcaag agcaaagcca
     1501 attcgagcat gtccgagacg ccttgcgcct tcgagatgat gctggttcgg atcgcctcct
     1561 cgccatagac gcggcggatt tcagcggcgg tcttgaagac ggcgagctcg ccttgcgtct
     1621 cctcgctata ggcgaggaac ggcgagacga gcggccgcgg attggcgagc tccttcaaca
     1681 acagcgcgac gcggcccttc tcgttcaacg cgagataatc gacgccgggc gccgccttgg
     1741 cgaaaagctc ggcgacggtg cgctcatgca cgtcggaatt ctgcctgaga tcgactggcg
     1801 cgagatgaaa accgaaacaa tcgacggcgc ggcgcaggcg acggagccgc ccccgcgcga
     1861 gaatggccgc gccgttttct tcgagcgagc gggcaatgat ttcgagatcg gcggccaact
     1921 ccttggccga ggcataggcc ggcgcctcgg cgattggccg gcgcagagcg acgacctggt
     1981 cgagctcgcg cgccgtggcg gcgaggcgcg aatagacgcc gcctatggcg cggcggtagg
     2041 gctcgtcggc gcgcgtcatc gaccggtcgg tcgagcgctc ggcgagttcg gcgagcgccg
     2101 gagtgatagg agtcagcacc gcgctgagcg acaattcgcc gccaagctcg tgcaattcgt
     2161 cgaggtggcg gcggatcgcc gtcgcgcttt gcaggcgcgt cgtctcgcgc aggacatcgg
     2221 cggtgacgaa aggattgccg tcgcgatcgc cgccgatcca cgagccgacg cgcaggaacg
     2281 acgcgagcgg ctggttctcg ctcttgccgc cgcacgacag ttcgtcctcg agcgcggcgt
     2341 agaggcgcgg caattcggcg aagaacgtgt agtcgtaata agagagtccg ttggcgacct
     2401 cgtcgagcac tttcagccgg gtctggcgca gcatgctggt gcgccagagc gtcagcacgg
     2461 cgcggcgcag ccgctcttcg ctttcggcaa gctccggcgc gttgccggcc atgcgctcgc
     2521 gctggtcgat gatctcgccg atctcgatct cgcgggtcag catgctcttg cggcgcacct
     2581 cggtcggatg ggcggtgagg accgggctca tcagcgcgcc gttgaagaag gcgcgcagcg
     2641 cctcgtcgcc gatcccggct tgccgggcgc gcttcaacgc atgggcgagc gtgccggcgc
     2701 gcggcgacga cccggcgacg acatgggcgc ggttgcggcg ggtatgatgc tggtcctcgg
     2761 cgatattggc gagatgcgag aagtagctat aggcgcggac gatgatcagc gtctggtcgg
     2821 cagtcaggct gtcgagaatc gcctccaact cgcgccgcgc gctcatatcg ccgtcgcggt
     2881 gaaagcggat cgaagcttgc cgaatgcgct cgacgatgtc gaacagcgcg ccgccctcct
     2941 gttggcgcac cgtgtcgccg agaatgcggc cgagcagccg gatgtcttcg cgcagcggcg
     3001 agtccttgtc gggactgtcc ggcgcaggcg gcgcggcggc aggaaaagcg gcttccccca
     3061 gagcggcgat cgacaccaaa tcccccatca ttcgcgccgg cgcgagcctc cggcatgctg
     3121 cggcgcagca taggaccaag gagtcggcgg ggcgagccca aaagtcaaca ggcgatcgcc
     3181 gggcgaacga cgcgataccg ccgcagatcg tccccgttcc ggccgcaatg tgtggcttga
     3241 ttaaggagcg gcgagagccg atcgccgagg aggggcctga tgcgcagcgt gacggcgggc
     3301 ttggctcggc aagtcgtggt cgccgtcgcg atgagcgcgc ttctcatcgc cgggcttagc
     3361 gtgcggccca gcggcggcgc gcgcccgccg gccaacgccg ccgcagaaaa gccgattgcg
     3421 acgcgggcga tcgtgaccgc ggaagcgccg ccggccgcgc cggtgaaacg cgcggcggcg
     3481 aagctggccg ccgcgcggct gcgcctcgtc gcgccgacgc cgcggccgcg cccggccgat
     3541 ccggccgcca aagtcatcgc cgcggcctat gtcccgccgg ggcctgagcc gagcgcgccc
     3601 agcctcaaga agcggctctt cgcgccgatc ggcttcgttc gcgacaatgt cgcgcggctg
     3661 atctcttggc tgtgagaagg cggccggcag aaattccttc cttcagcgaa gcaggaaacg
     3721 gtaaaagagc gctgttgtct cagaaaaggg tcgagccttg agcgcctcct acgaaaagga
     3781 cgtgctattg tggtccgagg agcaagcgcg cttgctgcgc acgggccagt tcggccgact
     3841 cgatatcgaa catttggcgg acgagatcga agacgtgggc aggagcgaag agcgcgaatt
     3901 ggccagccgc atggccgttc tcttggctcg ccttctcaag tggcggttcc ggcctgaaat
     3961 gcgcacccct cgctggcgga agacgatcaa ccatcagcgc cagcgggtgg agctcatctt
     4021 aaaggcgacc ccaagcctca aaacggcgat gtccgacgcg gactggcaaa agggcgcatg
     4081 gttagacgca atcggccagg ccgcgcagga aaccggactc gacgaagccg tcttcccgga
     4141 aacctgcccc tgggagatga gagcgactct cgacgccgct ttctggccgg attgaaaaag
     4201 cgccggtctt ggcgcgacgg gcttaatcga aaattggcag aaagaattta agtcttccgc
     4261 ttccgcaacc gccgggagcg cttttttgtc cgcgacagcc gcagggcgtt ccgccgccgc
     4321 ttcagcgcca agcgagcgcc gcctccggac cggtcttcgc gccgagcggc tgatcccagc
     4381 cggcgcggcc ttggcggcct tggcgatcgg gctgtggcgg ccggccaata ccgacatttc
     4441 ctggctgctg accgtcaacg aacgcatcct cgacggcgcg acgccttata aggatgtgat
     4501 cgagctcaat ccgccggctt cgatcctcct ttatcggctt cccgtcctgg cggcgcattg
     4561 gctttcgctc cgcgccgaaa tcgccgtcgt cgctttcatc gcgctgctga tcggcggcgt
     4621 cctgatctac gcccgccggc ttctgtccag gtacgagctt tgcaatcccg ctcgccgcgc
     4681 cgtcttcctg ctcgtcgcgg cgctcgttct cgccatgctg ccctttgacg agatggcgca
     4741 gcgtgagcat ttcgccacga tattcgcgct gccctacgcg ctggtcgcca tggcgcgcgc
     4801 ggcggaaagg cgagcggcgc cgctcgacgc gatcatcgcc ggcgcgatga tgggtctttg
     4861 cgtcgccgtc aagccgcatt tcgcgttgtg cgcgctgctc gtcgccgccg ccgaggcttg
     4921 gcgggcgcgc gccgccggcg cgttcttccg aatcgaaaat ctcgccgctt tcgcggtcgc
     4981 cctcgcttac gtgatcggca gctttctttt ctttccctat ttcttctccg acgtcctgcc
     5041 gatggtgcgg gatctttatc tgccgattcg gctcggcgcg gcggggctga tcgcgcatat
     5101 cgcgccgatt ctggctctgc cgctatgcct ctgctggctc tttcgcgccg agccgcaagc
     5161 cggcggcgcg cgcattctct tgctgatcgc cgccggcttt ctcgccgcct atttcctgca
     5221 gggcaaaggc tggagctatc acgcctatcc ggcgctcgcc ttctcgcttc ttgcggccgc
     5281 ctgggcgctg ccgcaagcgg cggacgccgt cgcgcgcctc tcgccgaagc tcggcgcgtt
     5341 gctgctcgcc gcggcgctga tcgccccggc gccgagcttc ttccggcgcg acgagcggca
     5401 cgccgcgctc gccgcagcca ttgcgaagat cgccccgcat ccgaagctcc tcgcgctctc
     5461 cttccgccag agcctgggcc atccgctgac gcgcaatatc ggcggcgtct gggtcggccg
     5521 gtattggggc ctgtggccga ccgggctcgc cgtgctgatg aaggagcgcg ccggcgacga
     5581 tgcaaccttg cgcgccaaag cggacgccta tttcgagaaa gatcgcctcg cggcggcgga
     5641 ggacatcgaa caccagaagc cggatatcgt gctgatcgac gaaacgccgg gcttcgactt
     5701 caacgcatgg atcgccgctt cgccgcagct tgaagctgcc ctggccaggt acaagccgat
     5761 gcaggcgatc gacggcgtcg agattctcca gcgtcggaac agctgagccg tcattgcgag
     5821 gagcgcagcg acgaagcaat ccactcatag agcgccgctc tacgaatgga ttgcttcgct
     5881 tcgctcgcaa tgacggcggc cgctcaaatc acattgcgct ccagcagcgg ccggttggcg
     5941 gcgatgcgct cgaaagcgaa gggagaaaga tcgagcgtcg catagcggcc ctcgacgatg
     6001 agctcggcga ggccgcggcc gatcgccggc gattgctgca tcccatggcc tgaaaatccg
     6061 gcggcgagat aggcgttttc caggccgccc aagcgcccga caatggcgtt gtggtcgaaa
     6121 atgttgagat cgtagtggca ggcccaggcg cggccgggcc ttatcgcctc gaaagcggga
     6181 atgcgcgccg ccaaagccgg ccaaatgatg tcgtcgaaat gccgccagac gacatcgaaa
     6241 tcgtcgctgt cgggatcgtc ctcctccggc ggcgagacgc cgcaaacata gttttcgccc
     6301 tcaggccgga catagacgcc gctcgtgtcg atgatcagcg gacaatgagc caagcgctcc
     6361 ttggcggcga agacgaagac cgagcgcttg cgccgccgca ccggaatcgc gacgcccagc
     6421 atgtcagcga gttgggccgc gccggaggcg ccggcggcgt tgacgaaagc gccgcaggcg
     6481 atgcgctcgc cggtcgagag gcgcgccgcg acgacgcgcg cgccgtcgcg ttcgaacccg
     6541 atgatcacgg cttcgcggta ttcggcgccg aggctcttgg ccttgcggcg aaaggcctgc
     6601 atcagcgtat agccgtcgaa ccagccttcg ttcctgcggc cccaggcccc gagcgcgacg
     6661 ccgtcgagat tgaggaaggc aaagcgctcg gcgagggaag gcggttcgag aagaaggatg
     6721 tcggcgcctt ccgcgctttg gacaacatgg ttttccgcca ggattccggc gccctggccg
     6781 ctcgctaggt acagatagcc gttctcgcgg aacgagagtt cgggcgcgac gccgtcggcg
     6841 gccaaggttt ccttggcctt ccgcaggaag gcgacgccgt aaaggcccac cctgatgttg
     6901 accgcggtcg agaattgctg ccggatcgag ctgagcgaca gggcgctggc cgagcgctga
     6961 taggtcatgt ccttatcgac gacgaggacg cggccggaaa agccgggatg ggcggcgagg
     7021 tgataggcga tcgacgagcc catcaccgcg ccgccgccga taacgacgtc gaaggtttcg
     7081 ctcatgccga gcgcctcggc atttggctga gccggccctc atccggcgct tcgcgccacc
     7141 ttctcccgcg agcgggagaa gggttgtagc cgcggtcgcc gaccgcggct ccagcgcgac
     7201 gccgatgcga ccgcggcgag ccccctctcc cgcttgtggg agagggctgg cgcgaggggt
     7261 agccgcgatg agctgctttc gccggcatct gctataagcg ccccgatcaa gagaaggacc
     7321 ttgtcatgac gcgtctagcc ggaaaagccg ccatcgtcac gggggcggcg catgggatcg
     7381 gccttgcctg cgcgcgccgc ctcgccgcgg acggcgcggc ggtcgccctc gccgatatcg
     7441 acgccaaagc cggcgaagag gcggccaggg cgctgcgcga ccagggcgcg cgggcgattt
     7501 tcgtcgcgac tgacgtggcg cagcgtccgg ctctggcggc tttgatcgac cgcgcggcca
     7561 gcgaattcgg acggctcgac atcatgctca acaatgccgg cgtcgcgctc agcgcgccga
     7621 tcgtcgagat gagcgacgag ctgttcgaca aggtcctgtc gatcaatctg cgcgccgcct
     7681 ttgtcggcac gcagctcgcg gcgcgcaaga tgatcgcggc caagaatggc ggcgtgatca
     7741 tcaacatgtc gtcggtgaac gctctgctgg cgatccccgg cctcgccgcc tatgcctgca
     7801 ccaagggcgc gctcaaccag ctcaccaagg tcgcggcggt cgaactcgcc ccgcacaaga
     7861 tacgggtcgt ggcgatcggt cccggcacga tcctgaccga gctcgccaaa aaggcggtgc
     7921 tgagcgacga ggcggcgcgc aagaagattc tctcgcgcac gccgatcggc cgggccggcg
     7981 agccggagga ggtcgccgcc gtcgcctcgt tcctcgccag cgccgacgcc tcctacatca
     8041 ccggccagac catctatccc gacggcggcc ggctggcgct gaactacacc gtgccggcgc
     8101 cggactgagc ccgggccttt gttg
//
LOCUS       DATGJW010000399         2791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_388795, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000399 DATGJW010000000
VERSION     DATGJW010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2791
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_388795"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            44..934
                     /locus_tag="VKU03_12020"
     CDS             44..934
                     /locus_tag="VKU03_12020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007610992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ72037.1"
                     /translation="MQEPPAALAAGIAPSAARASAARRSNETRYIEAHDGTRLYWVES
                     GRGRPLLFLNSWAMTVQMWDLQVAAFAEQGFRCIRYDRRGHGRSDQPAEGYDYDTFAD
                     DLAAIIEALELHDLTLIGHSMAGGEIVRYLSRHGDTRIARAVFVAPTTPFPLKTDDNP
                     LGVPRENYETVRALWRRDFPKWVADNAAPFFTPETSPALMQWAVNILLGISLPVAIAV
                     NRAVTETDFRAEMTQIKIPVLILHGDRDASAPLEMSGKPSAALLPDCRLKVYEGGPHG
                     LMYTHVDRLHADILAFIDET"
     gene            1026..1265
                     /locus_tag="VKU03_12025"
     CDS             1026..1265
                     /locus_tag="VKU03_12025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-finger domain-containing protein"
                     /protein_id="HLJ72038.1"
                     /translation="MAEEHATPHFHNDPGVPTIKVGVKEFMCVGARPPFDHPHIFIDM
                     GDADETVCPYCSTRFVYDPSLGPHCSPRECELIDA"
     gene            1324..2505
                     /locus_tag="VKU03_12030"
     CDS             1324..2505
                     /locus_tag="VKU03_12030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0071949 - FAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent monooxygenase"
                     /protein_id="HLJ72039.1"
                     /translation="MSGRALIAGAGIGGLTAAIALGRAGFDIAIFERAERLEEIGAGL
                     QLTPNATRILDKLDLLETVERRALAPRAIRIFRGRDGAKLVRLPLEARERWGAPYLVL
                     RRADLQSALAGRAAAEPRIKLSLGMEAAGCGEDEAGVLLGLTRGAIRLAERGDLLIGA
                     DGISSAIRERLGFGDRNSARFSGRIAFRATIAAKAAPAWCGEPDVSLHLGAKAHLVHY
                     PVAGGAEINIVAVIEAGWRDRPGEDPWDGEADAAALRAAFAGWSKAARALIAAPREWR
                     AWPLRERPALDRLAVGRVALLGDAAHPMLPFLAQGAGQAIEDAGALAECLAASNDIAE
                     ALAAYSRRRAARVARVQRESETQARLYHMAGPLALARDFGMRALGAERLLRRYDWIYR
                     A"
BASE COUNT          438 a          979 c          925 g          449 t
ORIGIN      
        1 cgcatagtcc ctccgcaacg ctgatggaga gactgagatg cgcatgcaag aaccgcccgc
       61 ggcgctcgcc gccggcatcg cgccttccgc cgcgcgcgct tccgcggcgc gccgaagcaa
      121 cgagacgcga tatatcgaag cgcatgacgg aacgcggctc tattgggtcg agagcgggcg
      181 cggccgcccg ctgctttttc tcaacagttg ggcgatgact gtccagatgt gggatttgca
      241 agtcgcggct ttcgccgagc agggctttcg ctgcatccgc tacgatcggc gcggccatgg
      301 ccggtccgac cagccggccg aagggtacga ctatgatacg ttcgccgacg atctcgccgc
      361 gatcatcgag gcgctcgaac ttcacgacct caccctgatc ggccactcga tggccggcgg
      421 cgagatcgtg cgctatctta gccgccacgg cgacacgcgg atcgctcgcg ccgtctttgt
      481 cgcgccgacc acgccctttc cgctcaaaac ggacgacaat ccgctcggcg ttccgcggga
      541 gaattacgaa acggtccgcg ccctatggcg gcgcgatttt ccgaaatggg tcgccgacaa
      601 cgcggcgccg ttctttacgc cggagacgtc gccagccctg atgcaatggg cggtcaacat
      661 cctgctcggc atatcgctgc cggtggcgat cgccgtcaac cgcgccgtga ccgagaccga
      721 tttccgcgcg gagatgaccc agatcaaaat tccggtcttg atcctgcacg gcgatcgcga
      781 cgcctcggcc ccgctcgaaa tgtcaggcaa gccgtcggcc gcgctgctcc cggattgccg
      841 gctcaaagtc tatgagggcg ggccgcacgg cctgatgtac acgcatgtcg accgcctgca
      901 tgcggacatc ctggccttta tcgacgagac gtgacggccc gccaggcggc cggcaaaaac
      961 cgctattcaa gccggcccgc ctcgcctata tgggcggcgc aacgccacag ccgccggagc
     1021 gctccatggc cgaggaacac gccacgccgc atttccacaa cgacccaggc gttccgacga
     1081 tcaaggtcgg ggtcaaggaa ttcatgtgcg tcggcgcccg gccgcctttc gaccatccgc
     1141 atattttcat cgatatgggc gatgcggacg agacggtctg tccctattgc tcgacccgtt
     1201 tcgtctacga tccgtcgctc ggcccgcatt gctccccccg cgaatgcgaa ttgatcgacg
     1261 cataagggga ccgtcattgc gcgcgacgcg ccctgtcatt cccgggaatg acagaaaagc
     1321 cccgtgagcg gccgcgcgct gatcgccgga gcggggatcg gcggattgac ggcggcgatc
     1381 gcgctcggtc gggccggctt cgacattgcg attttcgagc gcgccgagag gctcgaggaa
     1441 atcggcgctg gcttgcagct cacgccgaac gcgacgcgga ttctcgataa gctcgatctt
     1501 ctggaaacgg tcgagcgccg cgcccttgcg ccgcgggcga tccggatttt ccgcggccgc
     1561 gacggcgcca agctcgtccg cctaccgctc gaagcgcgcg agcgctgggg cgcgccctat
     1621 ctcgtgctgc gccgcgccga tctgcagagc gctctcgccg ggcgcgccgc cgccgagcct
     1681 cggatcaagc tgtcgctcgg catggaggcc gccggctgcg gcgaggacga ggcgggcgtc
     1741 ttgctcggcc tgacgcgcgg cgcgatccgc ctggcggagc gcggcgacct gctgatcggc
     1801 gccgacggga taagctccgc gatccgcgaa aggctgggct tcggcgatcg gaacagcgcg
     1861 cgcttttccg gccgcatcgc cttccgcgcg acgatcgccg ccaaggcggc cccggcttgg
     1921 tgcggcgagc cggacgtttc gctccatctc ggcgccaagg cccatctcgt ccactacccg
     1981 gtcgccggcg gcgccgagat caacatcgtc gccgtcatcg aagcgggctg gcgcgaccgg
     2041 ccgggcgaag acccttggga cggcgaagcc gacgccgccg cgcttcgcgc cgccttcgcc
     2101 ggctggtcga aggcggcgag ggcgctgatc gccgcgccgc gcgaatggcg cgcctggccg
     2161 ctgcgcgagc gtccggcctt ggatcgcctc gccgtcggcc gcgtcgcgct gcttggcgac
     2221 gccgcccatc ccatgctgcc cttcctcgcg cagggcgccg gccaggcgat cgaggacgcc
     2281 ggcgccttgg ccgaatgctt ggcggcgagc aacgacatcg ccgaagcgct cgccgcctat
     2341 tcgcgccgcc gcgccgcgcg cgtcgcgcgg gtgcaaaggg aatcggagac gcaagcgcgg
     2401 ctgtaccata tggccgggcc gttggcgctg gcgcgggatt tcggcatgcg ggcgctcggc
     2461 gcggaaaggc tgctgcggcg gtacgactgg atatatcgcg cgtgaaccgt catcgcgagc
     2521 aaagcgaaac agcggtcatt tgcgagaacg ccgagcgcag cgaggcggcc gaaccaatcc
     2581 acgcatagag cgctgtccta tgggattgct tcgcttcggt cgcaatgacg gctagggttc
     2641 tttggaccac cacacgcggc acgccttctt cgtcgtcgga atgacgatcg cccgcggcag
     2701 ctccgcgccg gcgccgcacc ggccggcgga ctgcgcctcg tccaggtcga tcgatacata
     2761 ttttccgacc gtcactctca cccggtcagg t
//
LOCUS       DATGJW010000400         7457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1114099, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000400 DATGJW010000000
VERSION     DATGJW010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7457
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1114099"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..362)
                     /locus_tag="VKU03_12035"
     CDS             complement(<1..362)
                     /locus_tag="VKU03_12035"
                     /inference="COORDINATES: protein motif:HMM:NF016854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TfoX/Sxy family protein"
                     /protein_id="HLJ72040.1"
                     /translation="MDAAGLQKLFEPFAAVTVKGMFGGRGVYADGLMFALQAGEDIYL
                     KTDAISAPRFKEAGSRPFVYRSPMGPKETSYWLMPQEAARNPEALTAWCGLALETARR
                     VAAAKQEKGGRRAKPAPP"
     gene            complement(366..749)
                     /locus_tag="VKU03_12040"
     CDS             complement(366..749)
                     /locus_tag="VKU03_12040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929911.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly accessory protein"
                     /protein_id="HLJ72041.1"
                     /translation="MAAKSRFKVMTLTDAAAARVNEIIAGSDKPIVGLRVGVRNGGCA
                     GMSYTMEFAETVTPLDEVIEDKGVKVLIDPKAVLFLLGARMDYRTDRLSSGFVFENPN
                     ETSACGCGESVAITPASPEALAADA"
     gene            complement(866..1501)
                     /locus_tag="VKU03_12045"
     CDS             complement(866..1501)
                     /locus_tag="VKU03_12045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614301.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione binding-like protein"
                     /protein_id="HLJ72042.1"
                     /translation="MELYFSPLACSMASRIALYEAEASAKFTEVDPKTKTTLAGDDFL
                     AVNPLGLVPTIRLDNGDVLTENAAILQFIADRSPEAGLAPQGGMARSKLQQWLSFIGT
                     ELHKALFIPLFDKKMPEEGKARALEKGDKTLAYLNAYLEGREFLLDRLSVADAYLFTV
                     LNWKIATPVDLKKWPAIDAYYHRIKQRPAFARALSEEHERYAEEVRRHRAA"
     gene            1679..2245
                     /locus_tag="VKU03_12050"
     CDS             1679..2245
                     /locus_tag="VKU03_12050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ72043.1"
                     /translation="METFWKAGYAGTSLDDLAAATGMNRPSLYAAFGDKRSLYLKALR
                     HYWRMSAAEMREVLTEVRSLREALNEFYRRAVAIYCGVQGRPRGCFSVSTAPAAALED
                     EEIRIALAESVRRLDERLEALIRAAQGRGEIAATADPAALALLGTATLHTLAIRARAG
                     AAAEELEGMARKAVGAMCSVGGGTGLSS"
     gene            complement(2278..2913)
                     /locus_tag="VKU03_12055"
     CDS             complement(2278..2913)
                     /locus_tag="VKU03_12055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003506960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HLJ72044.1"
                     /translation="MPAMTLLAFALVAFVGIATPGPTVLLALSNGSRFGVGRACFGMI
                     GAVASDFVLIGAVSAGLGALLAASEFWFSVVKWIGVAYLAFLGIMLLRSNGTFDEALR
                     AAGEGRARSARAIFLKSFLVAVTNPKGYLFFTAFLPQFVAPAAPQAPQFFALALVFAT
                     IDFAVMLTYAALGSQAMRALKKSGARWLDRICGGALLALAGSLALYKRASA"
     gene            complement(2933..3367)
                     /locus_tag="VKU03_12060"
     CDS             complement(2933..3367)
                     /locus_tag="VKU03_12060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015684114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HLJ72045.1"
                     /translation="MERRNFFRAALAVLVGLATGGARAEAPRAQNVVYHLADLEKVGF
                     VLGNIHNHLEGAGGPAAIRIALVVHGRALAAFRADAHDQAVRAGAAQAAKAGVALYAC
                     SHTMAAMKLSLSDLMPGFAAAEKGGVVLLADLQAQGWAYLRP"
     gene            complement(3380..4012)
                     /locus_tag="VKU03_12065"
     CDS             complement(3380..4012)
                     /locus_tag="VKU03_12065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72046.1"
                     /translation="MRNSTMRAAAVAATLLSLAAPAAAGTAEDFIKRLLAREPAVGES
                     YACFDRLYDAAHLASHPQQNVRAMTLLVKYTIEAHPEPGEAPRYEVRIGVDFRKTKRM
                     KEVDGECSALHPEGDPTGPVKAHCAVACDGGSIDVALKDNGAMLVTIPDGAKTWTPGA
                     DDAEDEGDKAVLHGGFGPDDKLFRLDRAEMKRCLALAADKQEKAQILRQH"
     gene            complement(4238..4798)
                     /locus_tag="VKU03_12070"
     CDS             complement(4238..4798)
                     /locus_tag="VKU03_12070"
                     /EC_number="2.1.1.100"
                     /EC_number="2.1.1.334"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015317177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HLJ72047.1"
                     /translation="MLAVAAIWVLWLISWIGAALWADPAAKRPPAREELFYRIATVAG
                     AVLLALSRRIAGGRYWDFGLPGDWALVAIVVLGLAFTWWARLYLGRLWSSSVTRKADH
                     QVVETGPYALVRHPIYTGIIAAIFATAILKADPIAIVGALVMTFGFWLKARLEERFLR
                     EQLGAEAYDSYRRKVPMLLPFGPRSA"
     gene            complement(4829..5218)
                     /locus_tag="VKU03_12075"
     CDS             complement(4829..5218)
                     /locus_tag="VKU03_12075"
                     /inference="COORDINATES: protein motif:HMM:NF021438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2147 domain-containing protein"
                     /protein_id="HLJ72048.1"
                     /translation="MKRLGFTTTCLGAAALAGLLASSAQAGWTASGEWARDDGQVRVR
                     LSRCGGKICAVNTWARDPYGQEKAGDRFIMTLVSTDSSHWTGSAFDPQRHLVYTMDLS
                     VSGRQLTTRGCISGSTLCETAAWTRVR"
     gene            complement(5406..5963)
                     /locus_tag="VKU03_12080"
     CDS             complement(5406..5963)
                     /locus_tag="VKU03_12080"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010910733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016407 - acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar O-acetyltransferase"
                     /protein_id="HLJ72049.1"
                     /translation="MSNEGAVKTIYRRTPESAAMLANVKRAMAITTALNRLTFDDADE
                     IRALFSQLIGKKVDESFLLIPPFHTAGGDEIRVGRNVFVNQNCTFYDLGGLDIGDDVM
                     IGPNVNIITTGHPLEPSQRRAATIGKPIVIGRNVWIAAGATIIGGVTVGENSVVAAGS
                     VVTKDVPRNALVGGNPARLIRSIDG"
     gene            6200..7039
                     /locus_tag="VKU03_12085"
     CDS             6200..7039
                     /locus_tag="VKU03_12085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ72050.1"
                     /translation="MLRRIAEALERLAPPPPAAPDFAAAAAFVWHADAGRLAPVQKVN
                     RVELALLRGVDRVRDALVENTERFAKGLPANNALLWGARGMGKSSLVKAVHAEINRRD
                     SKSGLKLIEIHREDIESLPALMVLLRDAPFRFLVFCDDLSFDAADTSYKSLKAALEGG
                     IEGRPANVLFYATSNRRHLLPRDMMENERSTAINPGEAVEEKVSLSDRFGLWLGFHRC
                     SQDEYLAMVFGYADHFRLKAPADDIEREALEWATTRGARSGRTAWQFIQDLAGRLGQR
                     LDE"
     gene            complement(7077..>7457)
                     /locus_tag="VKU03_12090"
     CDS             complement(7077..>7457)
                     /locus_tag="VKU03_12090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72051.1"
                     /translation="DLSSLRKFALRFDRADRSRFLDRMARLDARRDARAPVAGPGGDA
                     ALLQEGQALEQAWRVEVQAFIVMKRRATPETAAALRAARAATGLIVRRIEAAPAATAE
                     GFKVKARAASWRRHGEPASAAARS"
BASE COUNT         1326 a         2523 c         2371 g         1237 t
ORIGIN      
        1 ttcggcggag ccggcttggc ccgccttccg cccttttcct gcttggcggc ggcgacccgg
       61 cgcgccgtct ccaaagcgag gccgcaccac gccgtcagcg cttccggatt cctcgccgcc
      121 tcttgcggca tcagccaata gctcgtctcc ttcggcccca tcgggctgcg atagacaaac
      181 ggccgggagc ccgcctcttt gaagcgtggc gcgctgatcg cgtcggtttt gagatagata
      241 tcctcgcccg cctgcagggc gaacatcagc ccgtcggcat agacgccgcg gccgccgaac
      301 atgcctttga ccgtgaccgc cgcgaagggc tcgaacagct tttgcaggcc ggccgcgtcc
      361 atgccttaag cgtcggcggc caaagcttcc gggctcgcag gcgttatggc gacgctttcg
      421 ccgcagccgc aggcggacgt ctcgttggga ttttcgaaga cgaagccgga agagagacga
      481 tcggttcgat aatccatccg cgcgccgagc aggaagagca cggctttggg gtcgatcagg
      541 accttaaccc ccttatcctc gatcacctcg tcgagcgggg tcacggtctc cgcgaactcc
      601 atcgtatagg acatgccggc gcagccgcca ttcctgacgc ccaccctgag gccgacgatc
      661 ggcttgtcgg agccggcgat gatttcgttg acccgcgccg ccgcggcgtc ggtcagggtc
      721 atcactttga agcgagactt cgcggccatc cttctctccc tcgcccgggt tcaaggccgg
      781 gcgagactct caggcgaata taacaatgcg ggccgccgat taacaggccc ggcggccggc
      841 tcgttcccag gggcgtcggc caaggtcagg ccgccctgtg ccgcctgacc tcctcggcgt
      901 agcgttcatg ctcttcggaa agcgcccgcg caaaagccgg tctttgcttg atgcgatgat
      961 aataggcgtc gatcgccggc cattttttca gatcaaccgg cgtcgcgatc ttccaattga
     1021 ggactgtgaa caggtaggcg tcggcgacgc tgagccggtc gagaagaaat tctcggcctt
     1081 ccagataggc gttcaagtaa gcgagggtct tgtcgccctt ttcgagcgcg cgcgccttgc
     1141 cctcctccgg cattttctta tcgaaaagcg gaatgaacaa cgccttgtgc aactcggtcc
     1201 cgatgaagct cagccattgc tgaagctttg agcgggccat gccgccctgc ggcgcgagcc
     1261 ccgcttccgg cgagcggtcg gcgatgaatt gcaggatcgc cgcgttttcg gtgagcacgt
     1321 cgccattgtc gaggcggatg gtcggcacca ggccaagcgg gttgacggcg aggaagtcgt
     1381 cgccggcgag agtcgtcttg gtcttcgggt cgacttccgt gaatttcgcc gacgcctccg
     1441 cttcataaag tgcgatgcgc gaggccatcg aacaggcgag cggcgagaaa taaagctcca
     1501 tttccgtctc ccattgattt ttgtatcggt tcgcatataa atatccggcg agaacgccct
     1561 gtcaagaatt atttgcgaaa tggtacaaaa tattccgaaa agcgaaagcg ccggcccggg
     1621 gcgccggccg cgcgggcgcc cgcgcgccta tgacccggat gcggcgctga gccgggtcat
     1681 ggagaccttt tggaaggccg gctacgccgg aacgtcgctc gacgatctcg ccgccgcgac
     1741 cggcatgaac cggccgagcc tctacgcggc tttcggcgat aagcggtccc tctatctcaa
     1801 ggcgctgcgc cattactggc ggatgagcgc ggccgagatg cgcgaagtgc tgaccgaagt
     1861 gcggtcgctg cgcgaggcgc tgaacgaatt ctaccgccgc gccgtcgcga tctattgcgg
     1921 cgtccagggc cggccgcgcg gctgcttttc cgtctccacc gcgcccgccg ccgcgctcga
     1981 agacgaggaa atccgcatcg ccctggccga gagcgtgcgc cggctcgacg agcggctgga
     2041 agcgctgatc cgcgccgcgc aggggcgcgg cgagattgcc gcaaccgccg accccgccgc
     2101 cttggccctg ctcgggacgg cgacgctgca cacgctcgcg atccgggcgc gcgccggcgc
     2161 ggccgcggag gagctcgaag ggatggcgcg caaggcggtc ggcgcgatgt gcagcgtcgg
     2221 cgggggcaca ggcctatcgt cataggccct tcgtcattgc gagcgagcga tgcaatccta
     2281 cgcgctcgcc cgtttgtaca aagccagcga tcccgcaagc gccagcagcg ccccgccgca
     2341 gatccggtcg agccagcgcg cgcccgactt cttgagcgct cgcatcgctt gcgatccgag
     2401 cgccgcgtag gtgagcatca cggcaaaatc gatcgtcgcg aagacgagcg ccagcgcgaa
     2461 aaattgcggc gcttgcggcg cggcgggcgc gacgaattgc ggcagaaaag ccgtgaagaa
     2521 cagatagcct ttcggattgg tcacggcgac gaggaagctc ttcaagaaga tcgcccgcgc
     2581 cgagcgcgcc ctgccctcgc ccgcggcgcg cagcgcctcg tcgaatgtac cgttcgagcg
     2641 cagcagcatg atcccgagga aggcgagata ggcgacgccg atccatttga cgacggagaa
     2701 ccagaattcc gaggccgcga gcagcgcgcc aagcccggcg gaaaccgcgc cgatcaacac
     2761 gaaatccgac gcgaccgcgc cgatcatgcc gaaacaggcg cggccgacgc cgaagcgcga
     2821 gccgttgctc aaagcgagca gcacggtcgg cccaggcgtc gcgatgccga caaaggcgac
     2881 gagcgcgaag gcgagcagcg tcatggcggg caagcggcga ctcctttaac gatcacggcc
     2941 tcagatacgc ccagccctgc gcctgcagat cggcgaggag gaccacgccg cccttctcgg
     3001 ctgcggcgaa gccaggcatg agatcgctca agctcagctt catcgccgcc attgtatgcg
     3061 agcaagcata gagcgcgacg ccggctttcg ccgcctgcgc ggcccctgcg cgaacggctt
     3121 gatcatgcgc gtcggcgcgg aacgcggcga gcgcccggcc atggacgacg agcgcgatcc
     3181 tgatcgccgc cggaccgccc gcgccttcga gatggttatg gatattgccg agtacgaagc
     3241 cgaccttctc gagatcggcg agatgataga cgacgttctg ggcgcgcggc gcctcggctc
     3301 gcgcgccgcc cgtggcaagg ccgaccagaa ccgccaaggc ggcgcgaaag aaattgcggc
     3361 gctccatcgg ctttatctct cagtgctggc gcagaatctg cgctttttcc tgtttatccg
     3421 cggcaagggc gaggcatcgc ttcatctcgg cgcgatcgag ccggaacaat ttgtcgtccg
     3481 ggccgaagcc gccatgcagc acggccttat ccccctcgtc ctcggcatcg tccgcgcccg
     3541 gcgtccaggt cttcgcgccg tcgggaatcg tcaccagcat ggcgccgttg tccttcaagg
     3601 cgacatcgat gctgccgccg tcgcaagcga cggcgcaatg ggctttgacc gggccggtcg
     3661 gatcgccctc cggatggagg gccgagcatt cgccgtcgac ctccttcatc ctttttgtct
     3721 tgcggaaatc gacgccgatg cgcacttcgt agcgcggcgc ttcgcccggc tcgggatgcg
     3781 cctcgatcgt gtatttcacc agcagggtca tcgcgcgaac gttctgctgc ggatgcgagg
     3841 cgagatgcgc cgcgtcatag agccgatcga aacaggcgta gctctcgccg accgccggct
     3901 cgcgcgcgag cagccgcttg atgaaatcct ccgccgttcc cgccgccgct ggagcggcca
     3961 acgacaacaa agtcgcggcc acggccgccg cgcgcatcgt cgagtttcgc ataggttgag
     4021 cccctccccc accgagaata acagaaacga ggaggattgg cagacccgaa gggcgctcga
     4081 tgacgcaaaa ttgcccggcc tttgtgccgg gcatccacgc ccatccgcag acgcaaccgc
     4141 gtggatggcc gggataagcc cggccatgac gtggttgagc aagtccgagc tcgcgacttc
     4201 cgcgcgtacc ttctttcgga ttcgtacttc tgctcggcta agccgatctc ggcccgaacg
     4261 gcagaagcat cggcactttc cgccggtagg aatcgtacgc ctcggccccc aattgctcac
     4321 gcaagaagcg ctcttcgagc cgggctttca gccagaagcc gaacgtcatc accagcgcgc
     4381 cgacaatggc gatcggatcg gccttgagga tcgccgtggc gaagatcgcc gcgataatgc
     4441 cggtatagat cggatggcgg acgagagcgt aagggccggt ctcgacgact tgatggtcgg
     4501 cctttctggt gaccgagctc gaccagagcc tcccgagata gagccgcgcc caccaagtga
     4561 aggccagacc gagcacgacg atggcgacca gcgcccaatc cccgggaagc ccgaaatccc
     4621 aataccgacc cccggcgatg cggcggctta gcgccagcag cacggcgccg gcgacggtgg
     4681 cgatccgata aaagagctcc tcgcgggcgg gaggccgctt ggcggccggg tcggcccaca
     4741 gcgccgcgcc gatccaggag atgagccaca agacccaaat cgcggcgact gcgagcatca
     4801 gcttcaccgc ggggcccctg atgatgactt atctcacgcg cgtccaagcg gcggtttcgc
     4861 aaagcgtgct gccgctgatg cagccgcggg tcgtcaattg gcggccgctc acgctcaaat
     4921 ccatcgtata gaccagatgg cgctgcggat cgaaggccga gccggtccaa tggctggaat
     4981 cggtcgacac gagagtcata atgaaccggt ctcccgcctt ttcctgccca taagggtccc
     5041 tggcccaggt attgacggcg caaatcttgc cgccgcagcg cgacagccgc acccgcacct
     5101 gcccgtcgtc gcgcgcccac tcgccgctgg ccgtccagcc ggcctgcgcc gaagaggcca
     5161 aaaggcccgc cagcgccgcc gcgcccaggc aagtcgttgt aaatcctaat cgcttcaccc
     5221 agcgctctcc tttgtcgcct cgctccgatt gatcctgctt cagccgcaag aatctcaaga
     5281 caagatttgt tccggatatc aagccaaatt ttgctgagat cagggcggcg acgcgagaaa
     5341 atcgggaaaa gtgacgccgg gctggccgaa gatcgccggc gggcgacgcc ctgaacgtca
     5401 agccgtcagc cgtcaatcga acgaatgagc cgcgccggat tccccccgac aagcgcattc
     5461 cggggaacgt ccttggtgac gaccgaaccc gccgccacga ccgagttttc gccgaccgtc
     5521 accccgccga tgatcgttgc gccggcggcg atccagacgt ttctcccaat cacgatgggc
     5581 ttgccgatcg tcgcggcgcg gcgctgcgaa ggctcaagcg gatggccggt cgtgatgata
     5641 ttcacgttcg gcccgatcat cacatcgtcc ccaatgtcga gcccgccaag atcatagaag
     5701 gtgcagttct gattgacgaa gacattgcgc ccgacgcgga tttcgtcgcc gccggcggta
     5761 tggaaaggag gaatcagcag aaagctttcg tcgacctttt tgccgatgag ctggctgaac
     5821 aaggcgcgaa tctcgtctgc atcgtcgaat gtcaagcggt tcagggcggt ggtgatcgcc
     5881 atcgctcgct tgacgttcgc caacatcgcc gcggattccg gcgtcctcct gtagatcgtc
     5941 ttgacggcgc cctcgttcga cattcgtcat tttccacatt gaggacgcgt tttccgcagg
     6001 cggcggccga gatttcgcca gcgtagccat gtggcggagc ggccagaaac cacgcggtct
     6061 tgaaattcac accggccagc cgctggagct tgctggcgga ctgtgccaaa ccgcctgaaa
     6121 cgcaatgacg acgaatcgca tgcccgacaa gccgctccgc tccgtttttc ccccaacttc
     6181 cgcagaaccg gccgacccga tgctccgccg catcgccgag gcgctggagc ggctcgcgcc
     6241 gccgccgccg gccgcgccgg atttcgccgc cgcggcggcc tttgtctggc atgccgacgc
     6301 cggccggctg gcgccggtgc agaaggtcaa ccgggtcgag cttgcgctgc tgcgcggcgt
     6361 cgaccgggtg cgcgacgcgc tggtcgagaa caccgagcgc ttcgccaagg gcctgcccgc
     6421 caacaacgcg ctgctctggg gcgcgcgcgg catgggcaag tcctcgctgg tcaaggccgt
     6481 tcacgccgaa atcaaccggc gcgacagcaa gagcgggctc aagctcatcg aaatccaccg
     6541 cgaggacatc gagagcctgc cggccttgat ggtcttgctg cgcgacgcgc cgttccgctt
     6601 tctcgtcttc tgcgacgatc tctccttcga cgcggccgat acgtcctata aatcgttgaa
     6661 agcggcgctt gagggcggca tcgaggggcg gccggccaat gtgctcttct acgccacgtc
     6721 gaaccgccgt catctcctgc cgcgcgacat gatggagaac gagcgctcga cggcgatcaa
     6781 tcccggcgag gccgtcgagg agaaagtctc actgtccgac cgtttcggcc tgtggctcgg
     6841 cttccaccgc tgcagccagg acgaatatct cgccatggtg ttcggctatg ccgaccattt
     6901 caggctgaaa gcgccggccg acgacatcga gcgcgaggcg ctcgaatggg cgacgacgcg
     6961 cggcgcccgc tcgggacgaa cggcctggca attcatccag gaccttgccg gccggctcgg
     7021 ccaaaggctc gacgaataag ccttaaaggc gcgagcagcc ggtccggtcg cttgggtcac
     7081 gatctggcgg cggcgctcgc cggctcgcca tggcgccgcc acgaagcggc gcgcgcctta
     7141 accttgaagc cttcggccgt cgccgcgggc gcggcttcga tgcgccggac gatcagcccc
     7201 gtcgccgctc tcgccgcccg cagggcggcc gccgtctcgg gcgtcgcccg ccgcttcatc
     7261 acgatgaagg cttggacctc gacccgccaa gcctgctcca gcgcctggcc ctcttgcaga
     7321 agggccgcat cgccgccggg cccggcgaca ggcgctcgcg catcgcgccg ggcgtcgagc
     7381 cgcgccattc ggtcgaggaa cctgctccgg tcggcgcggt caaagcgcag cgcgaacttt
     7441 cgcaggctcg agagatc
//
LOCUS       DATGJW010000401         2716 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1215144, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000401 DATGJW010000000
VERSION     DATGJW010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2716)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2716)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2716
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1215144"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VKU03_12095"
     CDS             <1..783
                     /locus_tag="VKU03_12095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ72052.1"
                     /translation="GLLAGGALTDRRPFRSPHHSASMPALVGGGVHAKPGEISLSHNG
                     VLFLDELPEFQPQVLDSLRQPLETGEVAIARANHRVVYPARFQLVAAMNPCRCGMANE
                     PGYACKRAPNDRCVAQYLGRLSGPLIDRFDLVVDVPSVSAADLMTPPAREGSAEAAAR
                     VMAARKIQLARYAGLGLDGVVCNAGAPASAIEEIAEADASGLALLRHAAERLRLSARG
                     YHRVLKLARTLADLDGGGPTAREHLAEALSYRGAPDHAAAAA"
     gene            904..2682
                     /locus_tag="VKU03_12100"
     CDS             904..2682
                     /locus_tag="VKU03_12100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ72053.1"
                     /translation="MDLGAGLADFAVHALWLQAGAMIALAIGLAAAVARARARTEARA
                     ARIAAENEGLRDELWRLKEAAGERDRAEAASEAKSRFLATMSHEVRTPISGILGMADL
                     LREASLGPENASYVEAIRSSGSALIALIDEILDLAKIEAGRFDLVVETVDLRRLAEGV
                     VELLAPRAQSKGIEIAASFSAAAPRFVRGDGLRLRQVLMNLAGNAVKFTDRGGVCVTV
                     ERGRTGAAQFSVIDTGPGVPADRRAAIFDSFEQGDGSHARRFEGAGLGLAISRELVRL
                     MGGEVTLADNPGGGSIFAFALRLPECAASEPLASEERRSAPEGTRALIIADSPFEAPA
                     MSARLEEAGVSVERATGLDSGLEALSGPEKPDVVIVDCALGPEATNRLARAAREAGVR
                     RSVLLFSPFERRAFGQTALKGFDGWLVKPVRTRSLFERVAWGVPPAAGPPPRLSPSAA
                     VRRALVAEDNDINALIAQKALRRLGFDVVRASDGEEAVRLAAPVAPLESRPFDVILMD
                     LKMPGLDGYEATRRLRRLEAGCGWSPTPIVALTANAFDERRGSPSAAGFDAFLVKPVE
                     FAALAGTVERVCAAGTAPWRQRLRAS"
BASE COUNT          368 a          963 c          962 g          423 t
ORIGIN      
        1 ggcctgctgg cggggggcgc gctgaccgac cggcgcccgt tccgcagtcc ccatcattcg
       61 gcctcgatgc cggcgctggt cggcggcggc gttcacgcca agcccggcga gatctcgctc
      121 tcccacaacg gcgtcctgtt cctcgacgaa ctgccggagt tccagcctca ggtcctcgac
      181 agcctgcgcc agccgctcga gaccggcgaa gtggcgatcg cgcgcgccaa tcatcgcgtc
      241 gtctatccgg cgcggtttca gttggtcgcg gcgatgaatc cgtgccgatg cggcatggcc
      301 aacgagccgg gctacgcctg caagcgtgcg cccaacgacc ggtgcgtcgc gcaatatctc
      361 ggccgcctct ccggcccgct catcgatcgc ttcgaccttg tcgtcgacgt gccgtcggtc
      421 tcggccgcgg acctgatgac gccgccggcg cgcgagggct cggccgaggc cgccgcccgg
      481 gtcatggcgg cgcgcaagat ccagctcgcg cgctatgccg ggcttggact ggacggcgtc
      541 gtctgcaacg ccggcgcgcc ggccagcgcc atcgaggaga ttgcggaagc ggacgcctcc
      601 ggcctcgcgc tgttgcgcca cgccgccgag cggctgcgtc tttcggcgcg cggctatcat
      661 cgggtgctca agctcgcccg caccctcgcc gacctggacg gcggcggtcc gaccgctcgc
      721 gagcatttgg cggaggccct ctcctaccgc ggcgcgcccg accacgccgc cgcggcggcg
      781 taatggttag cgcggcgcaa acgcatagaa ttcgctccaa ttccgcaaag aaatcgcaac
      841 ggcggcctga aaccatggat cgcgcagccg atggcgctaa ggccgggctg gccaggattg
      901 cggatggatt tgggcgccgg gttggcggac tttgcggttc atgcgttgtg gcttcaggcc
      961 ggcgcgatga tcgctctggc gatcggcctt gcggccgcag tcgctcgcgc ccgggcgcga
     1021 acggaagcgc gcgcggcgcg gatcgccgcc gagaacgagg ggctgcgcga cgaactctgg
     1081 cgcctcaagg aggcggcggg cgagcgcgac cgggcggagg cggcgagcga ggccaagtcg
     1141 cgctttctcg cgaccatgag ccacgaagtc cgcaccccga tcagcggcat tctcggcatg
     1201 gccgaccttt tgcgggaggc ctcgctcggc cccgaaaacg ccagctacgt cgaagccatc
     1261 cgcagctcgg gaagcgccct gatcgcgctg atcgacgaga tcctcgatct ggccaagatc
     1321 gaggcaggcc gtttcgacct cgtcgtcgag acggtcgatc tgcgccggct ggccgagggc
     1381 gtggtcgaac tgctcgcgcc gcgcgcccag agcaaaggca tcgagatcgc cgcctcgttc
     1441 agcgccgcgg cgccccgctt cgttcgcggc gacggcctgc gcttgcgcca agttctcatg
     1501 aacctcgcgg gcaatgcggt caaattcacc gaccgcggcg gcgtctgtgt gacggtcgag
     1561 cgcgggcgta cgggcgccgc ccaattctcg gtgatcgaca ccgggcccgg cgtgccggcg
     1621 gaccgcaggg cggcgatttt cgacagtttc gaacagggcg acggctcgca cgcgcgccgc
     1681 ttcgagggcg cgggactagg gctcgcgatc tcgcgcgagc ttgtccgtct catgggcggc
     1741 gaagtcaccc ttgccgataa tccgggcggg gggtcgatct tcgctttcgc cctccgcttg
     1801 ccggaatgcg ccgccagcga gccgctcgcc tcagaggaga ggcggagcgc cccggaggga
     1861 acgcgcgccc tcatcatcgc cgattctccg ttcgaggcgc cggcgatgag cgcccgcctt
     1921 gaggaagcgg gcgtcagcgt cgagcgcgcc accggcctcg attccggcct cgaagcgttg
     1981 agcggccctg agaagcccga cgtcgtcatt gtcgattgcg cgctggggcc ggaggcgacc
     2041 aatcgactcg cccgagccgc gcgcgaggcg ggggtccggc ggagcgtcct gctgttttcg
     2101 ccattcgaaa ggcgggcctt cgggcagacc gcgctcaagg gtttcgacgg ctggctggtc
     2161 aagccggtcc gcacgcgctc gctctttgag cgcgtagcgt ggggcgttcc gcccgccgcc
     2221 ggcccgccgc cgcgcctctc tccctcggcg gcggtccgcc gcgcgcttgt cgccgaggac
     2281 aacgacatca acgccctcat cgcccaaaag gcattacggc gcctcggctt cgacgtggtg
     2341 cgtgcgagcg acggcgagga ggcggtgcgt ctggctgcgc cggtcgcgcc gctcgagtcg
     2401 cgtccgttcg acgtcatcct gatggatctc aagatgccgg gtctcgacgg atacgaggcc
     2461 acgcgccgtt tgcgccggct cgaggccggg tgcggctggt cgcccacccc gatcgtcgcc
     2521 ctcaccgcca acgcgttcga cgagcggaga ggctctccct cggcggccgg tttcgacgcg
     2581 ttcctcgtca agccggtcga gttcgccgct ctggcgggga cggttgagcg ggtttgcgcg
     2641 gccgggaccg cgccgtggcg ccagcgcctc cgcgcgtcct gaaaaaggcc tgggtaacca
     2701 tcccgagcta tgcggc
//
LOCUS       DATGJW010000402         3606 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1009766, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000402 DATGJW010000000
VERSION     DATGJW010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3606)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3606)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3606
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1009766"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..90
                     /locus_tag="VKU03_12105"
     CDS             <1..90
                     /locus_tag="VKU03_12105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018645309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-6-phosphate isomerase"
                     /protein_id="HLJ72054.1"
                     /translation="DGEAVVVNLDIEPVEKPENVPWIDPIHKG"
     gene            complement(195..668)
                     /locus_tag="VKU03_12110"
     CDS             complement(195..668)
                     /locus_tag="VKU03_12110"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HLJ72055.1"
                     /translation="MSKSHSHSDSARRDWARQVEKFCAERDLQLTPLRRQVLDILAHA
                     LAPLGAYAIIEGLSRAQGKTVAPPTVYRTLDFFLEHGFLHKVESRNAYAPCEHIGHRH
                     QGILLICERCGRTDEIESPRFDKMLQETAAKAGFLAQRQVVEVEGLCEACGHAAH"
     gene            918..1796
                     /locus_tag="VKU03_12115"
     CDS             918..1796
                     /locus_tag="VKU03_12115"
                     /inference="COORDINATES: protein motif:HMM:NF013465.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ABC transporter substrate-binding protein"
                     /protein_id="HLJ72056.1"
                     /translation="MKKVAPLASAIIAAALLGSPALADPVSIVAAENFYGDVAAQVGG
                     DRVAATSILSNPDADPHLFEASAATARALANAKIVIANGADYDPWMEKLLKANKAPGR
                     REIVVADLAHKKPGANPHLWYDPSVVKALAAALAADLSVLDPIHKTEYEKGSAAFAAS
                     LEPLEARIAAMRRKYSGQPVAASEPVFGYMAALIGLNMREEKFALAVMNNTEPSAGDV
                     AAFEDDLKGHRVKAMLYNAQASEPAVQRLMRIAKDEKIPVVGVAETEPANMNYQQWMS
                     SQLDGLDRALSGGATQ"
     gene            1793..2656
                     /locus_tag="VKU03_12120"
     CDS             1793..2656
                     /locus_tag="VKU03_12120"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72057.1"
                     /translation="MTALRFDEVAIRLGGRTILSAADFAIEEGEFVGMLGANGAGKTT
                     LMRASLGLVPLAAGAISVLGAPARRGNPLIGYMPQNRGPIAGLRLTGYDMVASGAKGA
                     HWGLPRLDSALRREIDWALDMVDARKIAGRSLNEISGGERQRLLLSQALIGRPKLLLL
                     DEPLISLDPGHQKSVVETVKRLAAELKIAVLFSAHEINPLLHAIDRVLYLGAGKAALG
                     AVDEIITGPTLSRLYGAEIEVLRVGGRIFVMSGDFDMERDPHRHENGGHDHHHDGHGH
                     DHAGSADKVGA"
     gene            2674..3447
                     /locus_tag="VKU03_12125"
     CDS             2674..3447
                     /locus_tag="VKU03_12125"
                     /inference="COORDINATES: protein motif:HMM:NF013143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HLJ72058.1"
                     /translation="MRAAFAASGIVAILAGLVGFFLVLRGQTFAGHALSHVGFAGATG
                     AVLFGLSPLAGLVGFTVIAGCAMGALGERLSQRDVAIGMMLSLALGLGLLFLHFYTSY
                     AAQAAALLFGNVLGVDNAALIALAALAALSLAALGFIARPLLFASLQPELAEARGVNV
                     RVVSIAFLIIVAIAVAECAQIVGVLLVFTLMVGPAAAALAFTRRLYAAVALAALLALA
                     EAWGGLTLAYYSDWPTSFWITALSGVVYFASLVAARRSA"
BASE COUNT          562 a         1217 c         1164 g          663 t
ORIGIN      
        1 gacggcgaag ccgtggtcgt caatctcgac atcgagccgg tcgagaagcc ggaaaacgtg
       61 ccgtggatcg acccgatcca taaggggtga gcccctcatt gcgaggagcg cagcgacgaa
      121 gcaatccctt cataaggcgc agcgccctac gaatgaattg cttggcttcg ctcgcaatga
      181 tggcgtctct tccgtcaatg cgcggcgtgg ccgcaggctt cgcacagtcc ctcgacctcg
      241 acgacttggc gctgcgccaa gaagcccgcc ttggccgccg tttcttgcag catcttgtcg
      301 aaacgcgggc tttcgatttc atcggttcgc ccgcagcgct cgcaaatcag cagaatgccc
      361 tgatggcgat ggccgatatg ctcgcaaggg gcataggcgt tgcggctctc gaccttgtgc
      421 aggaagccgt gctccaggaa aaagtccagc gtgcgataga cggtcggcgg cgcgaccgtc
      481 ttgccctgcg cgcgcgacag cccctcgatg atcgcgtagg cgccgagcgg cgcaagcgcg
      541 tgcgccagaa tgtcgagcac ctggcgacgc agcggcgtca gctgcaaatc gcgctcggcg
      601 cagaatttct cgacctgccg cgcccaatcg cgccgggcgc tgtcgctgtg actatggctc
      661 ttgctcatcg catctcgtca gggcgcatcc tcccccgtga agcgaagcgg aacgggggag
      721 ggggaccgcg cgtagcgcgg tggagggggc ccggtctctc aggataatcc tcaatcctcg
      781 caggccccct tcaccatgct tcgcatggtc cccctccccc gcttcgcggg ggaggattgg
      841 cttgcctagc gcactgttat aacataacag accgattgcg gcggcgccag tccgcgcgat
      901 cctcggaggc ttgacgcatg aaaaaggtcg cccctcttgc gagcgccatt atcgccgctg
      961 cgcttctcgg cagcccagct ctggccgatc cggtcagcat cgtcgcggcc gagaacttct
     1021 atggcgacgt cgcggcccag gtcggcggcg atagggtcgc cgcgactagc attctgtcca
     1081 atcccgacgc cgacccgcat ctcttcgagg cgagcgccgc caccgccagg gcgctcgcca
     1141 acgccaagat cgtgatcgcc aacggcgccg attacgatcc ctggatggag aagctgctca
     1201 aggcgaacaa ggcgcccggc cggcgtgaga tcgtcgtcgc cgatctggcg cacaagaagc
     1261 cgggcgccaa tccgcatctc tggtacgatc cgtccgtggt caaagcgctg gcggccgcgt
     1321 tggccgccga tctctccgtc ctcgacccga tccacaagac cgagtacgag aaaggaagcg
     1381 ccgcgttcgc cgcctcgctc gagccgctcg aagcgagaat cgccgccatg cgccggaaat
     1441 attccggtca gccggtcgcc gcctcggagc cggtcttcgg ctatatggcg gcgctgatcg
     1501 gcctcaacat gcgcgaggag aaattcgccc tcgcggtgat gaacaacacc gagcccagcg
     1561 ccggcgatgt ggccgctttc gaggacgatc tgaaaggcca tcgggtcaag gcgatgcttt
     1621 acaatgcgca ggcgagcgag ccggcggtgc agcgccttat gcggatcgcc aaagacgaga
     1681 aaatacccgt tgtcggcgtc gccgagaccg agccggcgaa tatgaactat cagcagtgga
     1741 tgtcttcgca gctcgatggg ctcgatcgag ccctttccgg cggcgcgacg cagtgacggc
     1801 gcttcgtttc gatgaggtcg ccatcaggct cggcggccgg accatcttgt cggccgccga
     1861 ttttgcgatc gaggagggcg agttcgtcgg catgctcggc gccaacggcg ccggcaagac
     1921 gacgctgatg cgcgccagcc tcggccttgt cccgctcgcc gccggcgcga tcagcgtgct
     1981 cggcgcgccg gcgcggcgcg gcaatcccct gatcggctat atgccgcaga atcgcggccc
     2041 gatcgccggc ttgcgcctca ccggctacga catggtggcg agcggcgcca agggcgcgca
     2101 ttggggcctg ccgcggctcg attccgcctt gcgccgcgag atcgattggg cgctcgacat
     2161 ggtcgatgca cgaaagatcg ccggccgctc gctcaacgaa atttccggcg gcgagcgcca
     2221 gcgcctgctt ttgtcgcagg cgctgatcgg gcggccgaaa ttgctgctgc tcgacgagcc
     2281 gctgatcagc ctcgatccgg gccatcagaa aagcgtggtc gagacggtca agcgcctcgc
     2341 cgcggaattg aagatcgcgg tcctgttcag cgcgcacgag atcaatccgc tgctccatgc
     2401 gatcgatcgg gtcttgtacc tcggcgccgg caaggcggcg ctcggcgcgg tcgacgagat
     2461 catcacaggc ccgactctgt cgcgcctcta tggcgcggag atcgaggtcc tgcgcgtcgg
     2521 ggggcgcatt ttcgtcatgt ccggcgattt tgacatggag cgcgatccgc atcgccatga
     2581 gaatggcggc catgaccatc atcatgacgg tcatggccat gaccacgccg gctccgccga
     2641 taaggtcggc gcctgatgtt cgcttatgat ttcatgcgcg cggcgttcgc ggcctccggc
     2701 atcgtcgcca ttctcgccgg attggtcggc ttcttcctcg tgctgcgcgg ccagacattc
     2761 gccggccatg ctctgtcgca tgtcggcttc gccggcgcga ccggcgcggt gctgttcggc
     2821 ctctcgccgc tcgccggcct ggtcggcttc accgtgatcg ccggctgcgc catgggcgcg
     2881 ctcggcgagc gcttgagcca gcgcgacgtc gccatcggca tgatgctgtc gctggcgctc
     2941 ggcctcggcc tgttgttctt gcatttctac acatcctatg ccgcgcaggc cgcggctctg
     3001 ctgttcggca acgtgctcgg cgtcgacaat gcggcgctga tcgccctcgc ggcgcttgcc
     3061 gctcttagcc tcgccgcgct tggcttcatc gcccggccct tgctgttcgc gagcctgcag
     3121 ccggaattgg ccgaggcgcg cggcgtcaat gtgcgcgtcg tctcgatcgc cttcctgatc
     3181 atcgtcgcca tcgccgtcgc cgaatgcgcc cagatcgtcg gcgtgctgct ggttttcacg
     3241 ctgatggtcg gcccggccgc cgcggcgctc gctttcacaa ggcggctcta tgcggcggtg
     3301 gcgctcgccg ccctgctcgc cctcgccgaa gcctggggcg gcctgacgct cgcctattac
     3361 agcgactggc cgacgagctt ctggatcacg gcgctgtctg gcgttgtcta tttcgccagc
     3421 ttggtcgccg cccgcagaag cgcgtaattc ccttctcccg gtgaaacgca gggctgtccg
     3481 gggaaagtct gaggttatgg cgcgtcttgg acgtaatctt tcagcgccgc ggcgccacgc
     3541 cggccgatcg ccgtcaaccc gccatagcgc cggcaagcta cgtgcaagat catatccgct
     3601 tgtgcg
//
LOCUS       DATGJW010000403         7785 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_78162, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000403 DATGJW010000000
VERSION     DATGJW010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7785)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7785)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7785
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78162"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..446)
                     /locus_tag="VKU03_12130"
     CDS             complement(<1..446)
                     /locus_tag="VKU03_12130"
                     /EC_number="6.3.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010684691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004639 -
                     phosphoribosylaminoimidazolesuccinocarboxamide synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylaminoimidazolesuccinocarboxamide
                     synthase"
                     /protein_id="HLJ72059.1"
                     /translation="MDFLKPRLIPMNRRRRIYEGKAKVLYEGPEPGTLIQHFKDDATA
                     FNAKKHEVVDGKGVLNNRISEYLFQNLNDIGVPTHFIRRLNMREQLIREVEIIPLEVV
                     VRNVAAGSLATRLGIEEGTQLPRSIIEFYYKNDALNDPMVSEEHITA"
     gene            complement(642..1310)
                     /gene="modC"
                     /locus_tag="VKU03_12135"
     CDS             complement(642..1310)
                     /gene="modC"
                     /locus_tag="VKU03_12135"
                     /EC_number="7.3.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02142.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015412 - ABC-type molybdate transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum ABC transporter ATP-binding protein"
                     /protein_id="HLJ72060.1"
                     /translation="MIEIDAFHRQGDFTLQANVRAKGRALALCGASGAGKTTLLNIVA
                     GLVRPQAGRVEVNGVTLLDTKLGIDLAPRRRRVGYVFQELRLFPHLKVERNLLYGRRF
                     APKATPSVDFDQAVDLLGLRGLLTRWPARLSGGEKQRVAIGRALLANPQILLMDEPLA
                     ALDDARRMEILSYIEKLRDAFAIPIVFVSHRLSEVERLASDIAVLDHGKVAERRGGGR
                     ANGS"
     gene            complement(1307..2032)
                     /gene="modB"
                     /locus_tag="VKU03_12140"
     CDS             complement(1307..2032)
                     /gene="modB"
                     /locus_tag="VKU03_12140"
                     /EC_number="7.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015098 - molybdate ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015689 - molybdate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdate ABC transporter permease subunit"
                     /protein_id="HLJ72061.1"
                     /translation="MEQGASLSAILAPFTPQEWTAILLSLKVATVGTLVSLPFGLAVA
                     YLLARRSFPGKTLLNGIVHLPLVMPPVVTGWLLLLGFGRQGPFGHAFEACCGLVFSFR
                     WTGAALAAAVMGFPLMVRAIRLSIETVDTKLEQAAKTLGAPSPLVFATITAPLALPGI
                     VAGMILAFARAIGEFGATITFVANIPGETQTIATALYSATQIPGGEAGAARLTIVAIL
                     IAFAALVVAEILAARVSRAAMDQ"
     gene            complement(2132..2524)
                     /locus_tag="VKU03_12145"
     CDS             complement(2132..2524)
                     /locus_tag="VKU03_12145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1398 domain-containing protein"
                     /protein_id="HLJ72062.1"
                     /translation="MDKQVIAECMKASFADTPFPEVVARLAGAGVRAYRADLIKLRKT
                     YYDDAREAYDHAMPYGEAPDVAAQFSAGDVAGAVSAIQRREIGYAEFLKRIMQAGCAS
                     YGVYIGGRKAVYVGRDGDEYVEPFPAKP"
     gene            complement(2536..3003)
                     /locus_tag="VKU03_12150"
     CDS             complement(2536..3003)
                     /locus_tag="VKU03_12150"
                     /inference="COORDINATES: protein motif:HMM:NF024940.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ72063.1"
                     /translation="MSSANKAAALIPLIVADLFELAGAFRETGEAIARTTGQTQARWQ
                     VLSAASAERKTVPQIARRLGVARQGVQRLANLLVADGSAVFEPNPDHRASPHLVLTES
                     GRADLARLTKAAAAYHAAIARRMTGDDLAALRAGLKRLIEAAEQAAARSSREQ"
     gene            complement(3147..3920)
                     /locus_tag="VKU03_12155"
     CDS             complement(3147..3920)
                     /locus_tag="VKU03_12155"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012029084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016812 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in cyclic amides
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclase family protein"
                     /protein_id="HLJ72064.1"
                     /translation="MGRRLIDISVPLQNDVPADLGPGPKIAYIDHQQGLPALLSIFPG
                     LEAADLPDGQGWAVEQVALSTHNGTHLDAPWHFHPTMNRGERAATIDEIPLDWCLQPG
                     VKLDFRHFADGYVATAADVEAELKRIGHELKPLEIVVVNTAAGAKYGQPDYVPSGCGM
                     GYEATMYLLERGVRLTGTDAWSWDAPFVHTARKYAETKNAALIWEGHKAGRHIGYCHI
                     EKLHNLEALPATGFTIACFPVKIERASAGWTRAVAIIEE"
     gene            complement(3952..4941)
                     /locus_tag="VKU03_12160"
     CDS             complement(3952..4941)
                     /locus_tag="VKU03_12160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fumarylacetoacetate hydrolase family protein"
                     /protein_id="HLJ72065.1"
                     /translation="MKLATFRRDGAERVAIVHADDGKAFDLAAAAQRDGAANPAFDSM
                     LALIDADDRGLDAARALFERRGGESDLSYALGEIELRAPLPRPRQMRDGMSFETHIRQ
                     SSRGAMAAAARRKGGEAAFQAAMAAPLEDLAPVYRQIPIYYITNRMIVAGPGATIRWP
                     RYSEEMDYELELGMITKRTRANIPAAEAGAHIFGYTIFNDFSARDRQRLEMQGRLGPA
                     KGKSFDGANVLGPWIVTPDEIGDPQRLDVAVRVNGETRAKSTTQGMLNAFEEILAYAS
                     QDETIFAGEFFGSGTVGNCCGLETGRLLAHGDVIELEIEKIGVLKNKVVRQDA"
     gene            5026..5970
                     /locus_tag="VKU03_12165"
     CDS             5026..5970
                     /locus_tag="VKU03_12165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592616.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ72066.1"
                     /translation="MPIVRTFAARAAAFGAALLLGAPLAAAQTKIAIGCTATSDCASA
                     MVAADEGIFAKHGLDAKMVLIGINSNIPAAIVSDSIQIGGPTATVFLQAVDGGLDLVA
                     VAGASVMDPVANNAVAAYARNGTALHEAKDFVGKKVGAPGIGAFLQVLFVKWLTEKGV
                     DPKSVNFVEVTFPTQGDSLRSGAVDAVLTAEPFVTRFNAAKIGYVAAHYAAELGRTEP
                     IISYVAARSFAEKNPQAIAAFRAAIAEGAEIVNNDREKASESISKFTKQPLDIVKMNR
                     PSVSAPDVKPEGFVWWLDVMKQQNMLQNAVDPAKIVWK"
     gene            5967..6662
                     /locus_tag="VKU03_12170"
     CDS             5967..6662
                     /locus_tag="VKU03_12170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015665341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FCD domain-containing protein"
                     /protein_id="HLJ72067.1"
                     /translation="MSQAVLDKQTVGDTLSERAASLVERDIVAGAFAPGARLAIADLA
                     ERYAIGATPIREGLSRLVARGLIVAIGQRGFRVSEVSRADLEDITRLRSLIECEALRR
                     SMKLGDGAWEGAIVAALHQLRRYAQQNGAGLREGRADFDALHKSFHTALIAACGSPRM
                     IRAHSDLYDQAYRYRRVMMRTFEDASEFIKAHEELADLVLARRDREATAKLAAHLHST
                     LALVYPGETGSAP"
     gene            6659..7495
                     /locus_tag="VKU03_12175"
     CDS             6659..7495
                     /locus_tag="VKU03_12175"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007592620.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72068.1"
                     /translation="MTMKADALDADAAPPASSDRNAKGAPDPLIEFAGVSLAFSGKTV
                     LDNVDLSVGRGEIVCIVGPSGCGKTTALRLASGLLAPTAGRIAFNGAPLHGPRRDVAV
                     VFQDYGKALLPWRNAAGNVSLALEAAGVPRRLRASRIAALLAKVGLPHHADKYPSQMS
                     GGMQQRLQIARCLAQEPIALMMDEPFGALDAMTRQELQDELLNIVAASGATVFFVTHD
                     LEEAIYLGDRVIGLLPHPGRVGVELAIGLPRPRDQVATREAPEFLRLRRALYDFIKGA
                     ER"
     gene            7492..>7785
                     /locus_tag="VKU03_12180"
     CDS             7492..>7785
                     /locus_tag="VKU03_12180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008964107.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72069.1"
                     /translation="MKIDGRAFVLPIFLAILMELGARAHGPSDSLAPPSAIAAAFAEA
                     LGDGSLLTATRDTLASAFAGLALGGAIGLVLGVALGAAPAANRAFEAAIELTRP"
BASE COUNT         1318 a         2572 c         2560 g         1335 t
ORIGIN      
        1 gcggtgatgt gctcttcgga gaccatcgga tcgttcagcg cgtcgttctt gtagtagaat
       61 tcgatgatcg agcggggcaa ttgcgtgccc tcctcgatgc cgagccgcgt cgccagcgag
      121 ccggcggcga cattcctgac cacgacctcc agcggaatga tctccacctc gcgaatcaac
      181 tgctcgcgca tattgagccg gcggatgaaa tgggtcggaa cgccgatatc gttgagattt
      241 tgaaagagat attccgaaat tcggttattc agaacgcctt tcccatcgac gacctcatgc
      301 ttcttcgcgt taaaggcggt ggcgtcgtcc ttgaaatgct ggatcagcgt tcccggctcg
      361 gggccctcat agaggacctt ggccttgcct tcataaatgc ggcgacggcg gttcatgggg
      421 atcaaccgtg gcttgaggaa atccatgggt cggccttgtt tgctgggctt cgccgcgccc
      481 ttttggaggc gacgggcgaa atatggtgat tggggccgcg agagccaaca tttgaagtcg
      541 actcatgagg gctggacaag cccagggcca ccctaactga ttgcgacgga gcgcacaacg
      601 cgccttccgt cacgcctaat ttattttgat tttacaatga attagctccc gttcgcgcgg
      661 ccgccgccgc gcctctcggc cactttgcca tgatcgagca cggcgatgtc gctggccagc
      721 cgctcgacct cgctgaggcg gtgactgacg aagacgatcg gaatggcgaa ggcgtcgcgc
      781 agcttctcga tataggacaa aatttccatg cggcgggcgt cgtccagggc ggcgagcggc
      841 tcgtccatca gcaggatttg cggattggcg agaagcgcgc ggccaatcgc gacgcgctgc
      901 ttctcgccgc ccgacaggcg cgccggccag cgcgtcagca agccgcgcag gccgagcagg
      961 tcgaccgctt gatcgaaatc gacgctcggc gtcgctttcg gcgcgaagcg ccgcccgtag
     1021 agaaggttgc gctcgacctt gagatgcgga aacaggcgca gttcctggaa gacgtagccg
     1081 acccggcgac ggcgcggcgc aagatcgatc ccgagcttgg tatccagcaa cgtgacgccg
     1141 ttgacctcga cgcggcccgc ttgcggccgc accagcccgg cgacaatgtt gagcaacgtc
     1201 gtcttgccgg ccccggacgc gccgcacaga gcgagcgcgc ggcccttggc gcgcacgttc
     1261 gcttgcaagg tgaaatcgcc ttgacgatgg aaagcgtcga tctcgatcat tggtccatcg
     1321 ctgcgcgcga aacgcgcgcc gccaaaatct ccgcgaccac caaagcggcg aaggcgatca
     1381 gaatcgcaac gatggtgagc cgcgccgccc ccgcttcgcc gcccgggatt tgcgtcgcgc
     1441 tatagagcgc cgtggcgatc gtctgcgtct cgccgggaat gttggcgacg aaggtgatcg
     1501 tcgcgccgaa ttcgccgatc gcccgggcga aagcgaggat catgccggcg acgattcccg
     1561 gcaaggcgag cggggcggtg atcgtggcga acacaagcgg cgaaggcgcg ccgagagtct
     1621 tggcggcttg ctccagcttc gtatcgaccg tttcgatcga cagccgaatc gcccggacca
     1681 tcagcggaaa gcccattacc gccgcggcca acgccgcgcc cgtccagcgg aaggagaaga
     1741 cgagcccgca gcacgcttcg aaggcatggc cgaacggccc ctgccggccg aagccgagca
     1801 gcaacagcca gccggtgacg accggcggca tgaccagcgg cagatggacg atcccgttga
     1861 ggagcgtttt gccgggaaag ctgcgccgcg ccagcagata ggcgacggcg aggccaaaag
     1921 gcaggctgac caaagtgccg accgtcgcga ctttcagcga cagcagaatc gccgtccatt
     1981 cctggggggt gaagggcgca aggattgcgc ttaaactcgc cccctgctcc acgcccgacg
     2041 cctcgccttc acgcaacgag cggccgcgcc gcggccgcca tctatatctc ttcaaatata
     2101 gcgcagcggg gcggcgagga gcaattgcgg ctcagggctt agcgggaaac ggctcgacat
     2161 attcgtcgcc gtcgcggccg acatagaccg ctttgcggcc gccgatatag acgccgtagc
     2221 tggcgcagcc ggcctgcatg atgcgcttca agaattcggc atagccgatc tctcggcgct
     2281 gaatcgcgct gaccgcgccg gcgacatcgc cggccgaaaa ttgcgcggcg acgtccggcg
     2341 cctcgccgta aggcatcgca tgatcatagg cttcgcgcgc atcgtcgtaa taagtcttgc
     2401 gcagcttgat caggtcggcg cgataggcgc gcacgcccgc gccggcgagg cgcgcgacga
     2461 cttccggaaa cggcgtgtcg gcgaagctcg ccttcatgca ttcggcgatg acttgcttgt
     2521 ccatggtcct tcctttcact gctccctgga tgagcgggcg gccgcttgtt cggccgcctc
     2581 gatcaggcgc ttgagcccgg cgcgcaaggc ggcgagatcg tcgccggtca tccggcgggc
     2641 gatcgccgcg tggtaagccg ccgcggcttt ggtcaggcgg gcaagatcgg cgcggccgct
     2701 ctcggtcaag acgagatgcg gcgaagcgcg atggtcggga ttgggctcga aaaccgccga
     2761 gccgtcggcg acgaggagat tggcgagacg ctgcacgcct tgccgcgcga cgccgaggcg
     2821 gcgggcgatc tgcggcacgg tcttgcgctc ggcggaggcg gcgctcagca cctgccagcg
     2881 cgcctgggtc tggccggtcg tccgggcgat cgcctcgccg gtttcgcgga acgcgccggc
     2941 gagctcgaac agatcggcga cgatcagggg aatgagagcg gcggctttat tggcggacga
     3001 catgacgcct ttatgtcata atgacaataa gttgtcaata tcgcctgtca ctgcgaggtg
     3061 cgtagcgaga cgaagcaatc cattcgtaag gcggcgctcg accaatggat tgcttcgctt
     3121 cgctcgcaat gacggcgaag cgccgactac tcctcgatga tcgccacggc gcgggtccag
     3181 ccggcggagg cgcgctcgat cttcaccggg aagcaggcga tggtgaaccc ggtcgccggc
     3241 aaggcttcga gattgtgcag cttctcgata tggcaatagc cgatatggcg gccggctttg
     3301 tggccctccc agatcagcgc ggcgttcttc gtctcggcgt atttcctggc cgtgtggacg
     3361 aagggcgcgt cccagctcca ggcgtcggtg ccggtcaggc gcacgccgcg ctcgagaagg
     3421 tacatcgtcg cctcgtagcc catgccgcat cccgacggga cataatcggg ctggccgtat
     3481 ttcgcgcccg cggcggtgtt gacgacgacg atttcgagcg gcttcagttc atggccgatg
     3541 cgcttcagct ccgcttcgac atcggcggcg gtcgcgacat agccgtcggc gaaatggcgg
     3601 aaatcgagct tgacgccggg ctggaggcac cagtcgagcg gaatttcgtc gatcgtcgct
     3661 gcgcgctcgc cgcgattcat ggtcggatgg aaatgccagg gcgcgtcgag atgcgtgccg
     3721 ttatgcgtcg agagcgcgac ctgctcgacc gcccatccct gcccgtcggg caggtcggcg
     3781 gcttccaggc cgggaaagat cgacagcagc gccggcagcc cctgctgatg atcgatatag
     3841 gcgattttcg gccccgggcc gagatcggcc ggcacgtcgt tctgcagcgg cacggaaata
     3901 tcgatcagcc ggcggcccat tctcttctcc ccattcgcgt cgcccggccg gtcaagcgtc
     3961 ctggcggacg actttgttct tcaacacgcc gatcttctcg atttcaagct cgatcacgtc
     4021 gccatgcgcc agcaggcgtc cagtctcgag gccgcagcaa ttgccgaccg tgcccgagcc
     4081 gaaaaactct ccggcgaaaa tcgtctcgtc ctgcgaggcg taagcgagga tctcctcgaa
     4141 cgcgttgagc atgccctggg tcgtgctctt cgcgcgcgtc tcgccattga cgcgcaccgc
     4201 gacatcgagt cgttgcgggt cgccgatctc gtccggcgtg acgatccacg gcccaaggac
     4261 attggcgccg tcgaagctct tgcccttggc cgggccgagc cggccttgca tctccagccg
     4321 ctgacggtcg cgcgccgaga aatcgttgaa aatcgtgtag ccgaaaatat gcgcgcccgc
     4381 ttcggcggcc gggatgttgg cgcgcgtgcg cttggtgatc atgccgagtt cgagctcgta
     4441 atccatctcc tcgctgtagc gaggccatcg gatcgtcgcg cccggcccgg cgacgatcat
     4501 ccgattggtg atgtagtaga tcgggatctg ccggtagacc ggcgccaggt cttcgagcgg
     4561 cgccgccatc gccgcctgga acgccgcttc gccgcccttg cgccgcgccg ccgcggccat
     4621 ggcgccgcgc gaggattgcc ggatatgggt ctcgaaggac atgccgtcgc gcatctggcg
     4681 cggccgcggc agcggcgcgc gaagctcgat ctcgccgagc gcgtaggaaa gatcgctctc
     4741 gccgccgcgc cgctcgaaaa gagcgcgcgc cgcatcgagg ccgcgatcgt cggcgtcgat
     4801 caaggcgagc atggagtcga aggccggatt ggcggcgccg tcgcgctggg ccgcggcggc
     4861 aagatcgaac gcttttccgt catcagcatg aacaatcgcg acgcgttcgg cgccgtcgcg
     4921 gcgaaaagtg gcgagcttca ccggtcccct ccttgcaaag tcaaaaaata tatgatctta
     4981 atttttgtcg attttcaagc gacaatgctg ttcgggagga agcccatgcc gattgtcagg
     5041 accttcgccg cccgcgccgc ggctttcggc gctgccttgt tgctcggcgc gcccctcgcc
     5101 gccgcgcaga ccaaaatcgc gatcggctgc acggcgacgt ccgactgcgc ctcggcgatg
     5161 gtcgcggcgg acgagggcat tttcgccaag catgggctcg acgcgaaaat ggtgctgatc
     5221 ggcatcaatt cgaacattcc ggccgcgatc gtttccgatt cgatccagat cggcggtccg
     5281 accgcgaccg tgttcctgca agccgtggac ggcgggctcg atctcgtcgc cgtcgccggc
     5341 gcgtcggtga tggaccccgt cgccaacaac gccgtcgccg cctatgcgcg caacggcacg
     5401 gcgctgcacg aggccaagga ctttgtcggc aagaaagtcg gcgcgccggg catcggcgcc
     5461 ttcctgcagg ttctgttcgt caaatggctg accgagaagg gagtcgatcc gaaatcggtg
     5521 aatttcgtcg aagtcacctt cccgacccag ggcgactcgc tgcgctccgg cgcggtcgac
     5581 gccgtgctca cggcggagcc cttcgtcacc cgcttcaacg ccgccaagat cggctatgtc
     5641 gccgctcatt acgccgccga gctcggccgt accgagccga tcatctccta tgtggccgcg
     5701 cgcagcttcg ccgagaaaaa cccgcaggcg atcgccgcct ttcgcgccgc catcgccgag
     5761 ggcgcggaga tcgtcaacaa cgatcgcgag aaggcgagcg agtcgatctc gaaattcacc
     5821 aagcagccgc tcgacatcgt caagatgaat cgccccagcg tctcggcgcc ggacgtcaag
     5881 ccggagggct tcgtctggtg gctcgacgtg atgaagcagc agaacatgct gcaaaacgcc
     5941 gtcgatccgg cgaaaatcgt ctggaaatga gccaagccgt tctcgacaag cagactgtcg
     6001 gcgacacgct cagcgagcgc gccgcctctc tcgtcgagcg cgacatcgtc gccggcgcgt
     6061 tcgcgccggg cgcccggctc gccatcgccg atctcgccga gcgctacgcg atcggcgcga
     6121 cgccgatccg cgagggcctg tcgcgcctcg tcgcgcgcgg cctcatcgtc gcgatcggcc
     6181 agcgcggctt tcgcgtcagc gaggtctcgc gcgccgatct cgaagacatt acgcggctgc
     6241 gcagcctgat cgaatgcgag gccttgcgcc gcagcatgaa actcggcgac ggcgcctggg
     6301 aaggcgcgat cgtcgccgct ttgcatcagt tgcggcgcta tgcccagcaa aacggcgcgg
     6361 gcctgcggga agggcgggcc gatttcgacg ccctgcacaa gagcttccat accgccctca
     6421 tcgccgcctg cggctcgccg cgcatgatcc gggcgcattc cgatctctac gatcaggcct
     6481 atcgctatcg ccgcgtcatg atgcgtactt ttgaagacgc gagcgagttc atcaaggcgc
     6541 atgaggagct ggccgacctc gtcctggcgc gccgcgacag ggaggcgacg gcgaagctcg
     6601 ccgcccatct ccattcgacg ctggcgctgg tctatcccgg cgaaaccgga tcggcgccat
     6661 gacgatgaaa gccgacgcgc tcgatgcgga cgcggcgccg ccggcgtcga gcgatagaaa
     6721 cgcgaaaggc gcgcccgatc cgctgatcga attcgccggc gtcagcctcg ccttcagcgg
     6781 caagaccgtg ctggacaatg tcgatctttc ggtcggacgc ggcgaaatcg tctgcatcgt
     6841 cggcccttcc ggctgcggca agaccacggc tttgcgcctc gcttcgggat tgctcgcgcc
     6901 cacggcggga aggatcgcgt tcaacggcgc gccgctgcac gggccgcggc gcgacgtcgc
     6961 cgtcgtcttc caggattacg gcaaggccct gctgccctgg cgcaacgcgg ccggcaacgt
     7021 ctcgctcgcc ttggaggcgg ccggagttcc gcgccgcctg cgcgcgtcgc gcatcgccgc
     7081 tctgctcgcc aaggtcggcc tgccacatca cgccgacaaa tacccttcgc aaatgtcggg
     7141 cggcatgcag caacgcctgc agatcgcccg ctgcctcgcg caggagccga tcgcgctgat
     7201 gatggacgag ccgttcggcg cgctcgacgc catgacgcgg caggaattgc aggacgaatt
     7261 gctcaacatc gtcgctgcga gcggcgcgac cgtgttcttc gtcacccacg atctcgaaga
     7321 ggcgatctat ctcggcgacc gggtgatcgg cctgctgccg catcccggcc gcgtcggcgt
     7381 cgagctcgcg atcggcctgc cgcgcccgcg cgaccaagtg gcgacgcgcg aggcgccgga
     7441 attcctacgc ctgcggcgcg cgctctacga cttcatcaag ggcgccgagc gatgaagatc
     7501 gacggccgcg ctttcgtatt gccgattttc ctggccatcc tgatggagct cggcgcgcgg
     7561 gcgcatgggc cgagcgacag tttggccccg cccagcgcga tcgccgccgc tttcgcggag
     7621 gcgctcggcg atggctcgtt gttgacggcg acgcgcgata cgctggcgag cgcctttgcc
     7681 ggcctggcgc tgggcggcgc gatcggcctc gttctcggcg tcgcgctcgg cgccgcgccg
     7741 gcggccaatc gtgcgttcga agccgcgatc gagttgacgc gcccc
//
LOCUS       DATGJW010000404         6724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_285020, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000404 DATGJW010000000
VERSION     DATGJW010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6724
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_285020"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VKU03_12185"
     CDS             <1..1212
                     /locus_tag="VKU03_12185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis glucosyltransferase H"
                     /protein_id="HLJ72070.1"
                     /translation="DFVEAAFMRRAGYAVYMLPTIGGSYEESPPSLIDIATRDRRWCQ
                     GNLQHMRVLPAKGFKLVSRQHFISGVMGYVASPLWMAQLLVGIALVLQSHYIRPEYFT
                     SEFSLFPAWPRFDYERALELFAITMGVLLAPKLFGLLVALFSRRIRRACGGGLRLVAS
                     AIVETILSALFAPIMMVIQSGSVMQILFGRDTGWNPQRRDDGSIPFKSIVRRHRAHMA
                     LGVLTLFAGFLISPSLVAWMSPTIAGLILAIPLSWASGQLWIGVALRRIGLLTTPEES
                     TPPPIVQRANSLTLDLARNGHDREDGIRALHADAAFREEHDVLLPPAPRRKRGEFETD
                     IAMAAAKLNDAATLEDVLKWLTPKERMALLNDRALIALLARLPAERSQEKQPEPTAGA
                     ELIERVQRLAG"
     gene            1308..3401
                     /locus_tag="VKU03_12190"
     CDS             1308..3401
                     /locus_tag="VKU03_12190"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HLJ72071.1"
                     /translation="MALSIRASFAGLLFGAVPALLLAGTWLVAKTAVDHLLYRDAILT
                     SQNWTSYLVDNIKDLEDIAKGEKPSAESRAFFERAQKAGQVFRYVIYDPEGRTRFVSD
                     SLEKSLAEDEEAKPESAADHEAKAEGKRGGKPDQDDDDDENLAEHNPAAARAIAAGQP
                     LINAEEGEPPTRPQFFSEAYLPAIVNGRTIAIVETYIDQTRKRNEFRHTLILMSAALL
                     ALIAVAFGVPGFAWLKRGNEKRLAEAHIRFLAHHDSLTGLINRDKLREETDAALAKAD
                     GRFALHSIDIDHFKDVNDTLGHAAGDALIVAAARRLEELASEQDRVSRIGGDEFVLLQ
                     AGPLAEDSASALAAEIRRRLSLPYDLAGQPVSVTVSIGVAIAPAHGDSAARLMKSADL
                     ALFASKIGGRNKTSIFSAEMDKELAERLRLEKAIADALANSAFELFYQPIVEARTARL
                     LGFEALLRMRDATGAMVSPAVFIPLAEQMGLIDRIGEWVLREACRTAQAWPANLKVAV
                     NLSPAQFARAGLAGRIGTILDETGLNARRLELEITEGLLLSKSAEVLDELRKLKALGA
                     SIVMDDFGTGYSSLSYLWQFPFDKIKIDRAFMLALEAEDHGDAETIVRTIIDLGRSLK
                     VIVTVEGVENARQLDFVRAARCDQIQGYYFGRPMPRLDLAAYIATACAADLAAGSRPA
                     DAADARLGERPAANA"
     gene            3531..4355
                     /locus_tag="VKU03_12195"
     CDS             3531..4355
                     /locus_tag="VKU03_12195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008129834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfurtransferase/chromate resistance protein"
                     /protein_id="HLJ72072.1"
                     /translation="MSSINLISVDQLARLVGTPKCPVLIDVRPDGDFAADPRLIPGSV
                     RRPAASAQDWPDALQGRAAIVACRRGEALSHGAAAWLRHRGGQADALAGGVEAWALAG
                     LPMVLEAKLPRRDRDGRTVWVTRERPKIDRIACPWLIRRFVDPAAVFLFVKASEVIAI
                     GEQFQATPFDIEGVYWSHRGEKCTFDVMIEEFGLAAEPLLRLAAMVRGADTARLDLAP
                     EAPGLLAASLGLSRMYDDDLAQLEAGMSLYDAFYRWCRDATGETHNWPTNKPRSAR"
     gene            4406..5617
                     /gene="chrA"
                     /locus_tag="VKU03_12200"
     CDS             4406..5617
                     /gene="chrA"
                     /locus_tag="VKU03_12200"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HLJ72073.1"
                     /translation="MAKRPTDEDALAKQPSSGGAGELALYFLRLGLLGFGGPVALVGQ
                     MERELTGDRKWLSKEQMREAVALCQTLPGPLAIQVGIYIAYLRFGFWGAWIGGWTFIL
                     PNFLIVAALGALYVYFGDLKPVTAIFYGVSPAVIALILHSCYRLAKLGMEDRLQWAIA
                     AACFGVTVMLRAEVALLFLGAGAVGVLYYGKVIPRLAASRFALVAPIAAMSVAPAAQA
                     AGAGTLGKLALFFLKAGSLTFGSGLVIVPFLQQGLVEQYNWLDQRQFLIAVAIGMISP
                     GPVVITATFVGYLVDGFLGSLVSTIGIFLPSFILVLIAAPILARYRGNAYVQGFVKGA
                     YGAAIGTILGACILLGRIAIGDWLTALIGIASLAALFRWKVSNPLLMAATACVGLVAF
                     PAMQPDWVMVK"
     gene            5643..6536
                     /locus_tag="VKU03_12205"
     CDS             5643..6536
                     /locus_tag="VKU03_12205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1259 domain-containing protein"
                     /protein_id="HLJ72074.1"
                     /translation="MLRTIRLVLLVACGALTGVVFGADRDWSRVGDALGKAGSLQPGG
                     VYRVALPRTDLKVAVDGVTLKAGFALGGWIAFEPMGSEAMMMGDLVLAESEINPVMSK
                     LLVEGLQVTALHNHLLRAAPPIFYMHVAGKGDAEQLARAVRGALEQSKTPLGSAATPS
                     AAGADLGIDSAAIDRAIGFKGRNNGGVYQYSLPRSDSVKQDGMKMPPAMGVAIGINFQ
                     PTGDGRAAVTGDFVALANEVNPLIKALRENGIEVTAIHNHMLDDEPRVFFIHFWANDD
                     AVKLAKGLRAALDAAHVAPKG"
BASE COUNT         1125 a         2229 c         2135 g         1235 t
ORIGIN      
        1 gatttcgtcg aagccgcctt catgcgccgg gccggctacg ccgtctatat gttgccgacg
       61 atcggcggct cctacgagga aagcccgcct tcgctgatcg acatcgcgac gcgcgaccgg
      121 cgctggtgcc aaggcaacct gcagcatatg cgcgtgctgc cggccaaggg gttcaagctc
      181 gtctcgcgcc agcatttcat ttccggcgtg atgggctatg tcgcctcgcc gctctggatg
      241 gcgcaattgc tggtcggcat cgcgctggtg ctgcagtcgc actatatccg gccggaatat
      301 ttcaccagcg agttctctct gttcccggct tggccgcgct tcgactacga gcgcgcgctg
      361 gaactgttcg cgatcacgat gggcgtgctg ctcgcgccca agctgttcgg ccttttggtc
      421 gctttgttca gccgccggat tcgccgcgcc tgcggcggcg gcctgcgcct cgttgcgtcg
      481 gcgattgtcg agacgatcct ctcggccctg ttcgcgccga tcatgatggt gatccaatcg
      541 ggctcggtga tgcagatcct gttcggccgc gacaccggct ggaatccgca gcggcgcgac
      601 gacggatcga ttccattcaa gagcatcgtc cggcgccatc gcgcgcatat ggcgctcggc
      661 gtgctgaccc ttttcgccgg cttcctgatc tcgccgtcgc tggtcgcctg gatgtcgccg
      721 accatcgccg gcctcatcct cgccatcccg ctttcctggg caagcggaca attgtggatc
      781 ggcgtcgcgc tgcggcggat cgggcttttg accacgcccg aggaatcgac gccgccgccc
      841 atcgttcagc gcgccaacag cctcacgctc gatctcgccc gcaatggcca cgatcgcgaa
      901 gacgggatca gggctctgca tgccgacgcg gcctttcgcg aggagcatga cgtcctcctg
      961 ccgccggcgc cgcgccgcaa gcgcggcgag ttcgagaccg acatcgccat ggcggcggcc
     1021 aagctcaatg acgcagcgac gctcgaagac gtcctcaaat ggctgacgcc gaaggagcgc
     1081 atggcgctgc tcaacgaccg cgccctgatt gctcttctcg ctcgcctgcc cgccgagcgt
     1141 tcgcaagaaa agcagccgga gccgaccgcc ggcgccgaac tgatcgagcg cgtccagagg
     1201 ctggcgggtt aacgaactcc tgcagcggcg gttgcgccca cgtcgcccgc ttaagccaat
     1261 ctttattcaa catcttttaa agaggtcttt tcctctcgga aaagatcatg gctctttcca
     1321 tcagggcgtc ttttgccggg cttttgttcg gcgcggtgcc ggccctgctt ctcgccggaa
     1381 catggcttgt cgcgaaaacg gcggtcgacc atctcctcta ccgcgacgcc atcctgacga
     1441 gccaaaactg gacgtcctat ctcgttgaca atattaagga ccttgaagac atcgcgaagg
     1501 gggagaagcc ttccgccgag agtcgcgcct tcttcgagcg cgcccagaaa gccggccaag
     1561 tcttccgcta tgtcatctat gatcccgagg gccgcacgcg cttcgtttcc gacagcctcg
     1621 aaaagagcct tgccgaagac gaggaggcga aaccggaaag cgccgccgac catgaggcta
     1681 aggccgaggg caagcgcggc ggcaagcccg atcaggacga cgatgacgac gagaatttgg
     1741 ccgagcacaa tccggcggcg gcgcgagcga tcgcggccgg acagcccctg atcaacgccg
     1801 aagaaggcga gcccccgacg cggccgcaat tcttttcgga agcgtatctt cccgcaatcg
     1861 tcaacggccg aaccatcgcg atcgtcgaga cctatatcga ccagacccgc aagcgcaacg
     1921 agttccggca tactttgatc ctgatgagcg ccgccctgct cgcgctgatc gcggtcgctt
     1981 tcggcgtgcc cggcttcgcc tggctcaagc gcggcaacga gaagcgcctc gccgaagcgc
     2041 atatccgctt tctcgcccat cacgattcgc tcaccggcct catcaatcgc gacaagctgc
     2101 gcgaggaaac cgacgccgcg ctcgccaaag ccgatggccg attcgcgctg cattccatcg
     2161 acatcgatca tttcaaggac gtcaacgata cgcttggcca cgccgccggc gatgcgctca
     2221 tcgtcgccgc ggcccggcgg ctcgaagagc tcgcttccga gcaggaccgc gtctcgcgaa
     2281 tcggcggcga tgaattcgtc ctgctgcagg ccggtccgct cgccgaggac tcggcctccg
     2341 cgctcgccgc tgaaatccgg cggcggcttt cgcttcccta cgatcttgcc gggcaaccgg
     2401 tcagcgtgac ggtcagcatc ggcgttgcta tcgccccggc gcatggcgac agcgccgcgc
     2461 ggctgatgaa aagcgccgat ctcgccctgt tcgccagcaa gatcggcggc cgcaacaaga
     2521 catcgatctt ttccgcagag atggacaagg agctggcaga gcgcctgcgc ctcgagaagg
     2581 cgatcgcaga cgccctggcc aacagcgcgt tcgagctctt ctaccagccg atcgtcgagg
     2641 cgcgtacggc gcgcttgctc ggcttcgagg ccttgctgcg catgcgcgac gcgacgggcg
     2701 ccatggtctc gccggccgtc ttcattccgc tcgccgaaca gatgggcttg atcgacagga
     2761 tcggcgaatg ggtcttgcgc gaagcctgcc ggaccgccca ggcttggccg gcgaacctca
     2821 aagtggcggt gaacctttcg ccggcgcaat tcgcccgcgc cggcctcgcc ggccgcatcg
     2881 ggaccatcct cgacgaaacc ggcctcaacg cgcggcggct cgaattggag atcaccgaag
     2941 gcctccttct gtcgaagagc gccgaagtgc tcgacgagtt gcgcaagctc aaggcgctcg
     3001 gcgccagcat cgtcatggac gatttcggca ccggctattc gagcctcagc tacctttggc
     3061 aattcccgtt cgacaagatc aagatcgatc gcgctttcat gctggccctc gaggcggaag
     3121 accatggcga cgccgaaacg atcgtcagaa cgattatcga tctcggacgc tcgctgaagg
     3181 tgatcgtgac ggtggaaggc gtcgaaaacg cccggcagct cgattttgtc cgagccgcgc
     3241 gctgcgacca gattcaaggc tactatttcg gccggccgat gccgcggctc gatcttgccg
     3301 cttacatcgc aaccgcctgt gcggcggact tggcggccgg gagccggccc gccgacgcgg
     3361 cggacgcccg acttggcgag aggccggcgg ccaacgccta atcgcgccgc gcggcgccgt
     3421 tcttgacacg agcgccgatt tctcttgtaa tcgagccatg gggatggcga tctccccacg
     3481 aggcggcatg ccgaagcatc gcgtcctggt cgagccagag gacgcgcgcc atgtcgtcaa
     3541 tcaatctcat ctccgtcgat caactggcgc gccttgtcgg cacgccgaaa tgcccggttt
     3601 tgatagatgt acggccggac ggcgacttcg cggccgatcc gaggctgatc cccggctcgg
     3661 tccggcgtcc ggccgcatcg gcgcaggact ggcctgacgc tctgcaaggc cgggccgcta
     3721 ttgtcgcctg ccggcgcggc gaggcgctca gccatggcgc cgccgcctgg ctgcgccatc
     3781 ggggcggcca ggccgatgcg ctggccggcg gcgtcgaagc ttgggcgctg gcgggcctgc
     3841 cgatggttct cgaggccaag cttccgaggc gcgaccgcga cggccgcacc gtctgggtca
     3901 cccgcgagcg cccgaagatc gatcgcatcg cttgtccctg gctgatccgc cggttcgtcg
     3961 atcccgcggc ggtctttctg ttcgtcaagg cgtcggaagt gatcgcgatc ggcgagcagt
     4021 tccaggcgac gcctttcgac atcgaaggcg tctattggag ccatcgcggc gaaaagtgca
     4081 cgttcgatgt catgatcgag gaattcggcc tcgccgccga gcccttgctg cgccttgccg
     4141 cgatggtgcg cggcgccgac acggcgcgtc tcgatcttgc gccggaggcg ccggggctgc
     4201 tcgccgcttc gctcggcctg tcgcggatgt acgacgacga tctcgcgcaa ctcgaagcag
     4261 gcatgtcgct ttacgacgcg ttctatcgct ggtgccgcga cgcaaccggc gaaacccata
     4321 actggccgac gaacaagccg cgaagcgcgc gatagcgccg gctgtcgaac agaagcagcg
     4381 cgccgcaaga tcgcgagaaa ggaccatggc gaaacgcccg accgacgagg acgctctcgc
     4441 gaaacaacca tcgagcggcg gcgccggcga gctcgcgctc tactttctgc gcctcggcct
     4501 tctcggtttc ggcggcccgg tggcgctggt cggccaaatg gagcgcgagc tgacgggcga
     4561 tcgcaagtgg ctcagcaaag agcagatgcg cgaggcggtt gcgctatgcc agaccttgcc
     4621 ggggccattg gcgatccagg ttggcattta catcgcctat ctgcgcttcg gattctgggg
     4681 cgcctggatc ggcggttgga cttttatcct gccgaacttt ctcatcgttg cggcgctcgg
     4741 cgcgctctac gtctatttcg gcgacctgaa gcctgtgacg gcgatattct atggcgtcag
     4801 tcccgcggtc atcgcgctga tcctgcattc ctgctatcgc ctcgccaagc tcggcatgga
     4861 ggataggctg caatgggcga ttgcggccgc ctgtttcggc gtgaccgtca tgctccgcgc
     4921 cgaggtcgcg cttctttttc tcggcgccgg cgccgtcggc gtcctctatt atggaaaggt
     4981 catcccacgc ctcgccgctt cgcgcttcgc gctcgttgcg ccgatcgccg cgatgagcgt
     5041 tgcgcccgcc gctcaggccg ccggcgccgg gacgctcggc aaactggcgc tgttcttcct
     5101 gaaagcgggc tcgctgacct tcggcagcgg cctggtgatc gtaccgtttc tccaacaagg
     5161 actcgtcgag caatataact ggctcgatca acgacaattc ctcattgcgg tggcgatcgg
     5221 catgattagc cctggcccgg ttgtgatcac ggcgactttc gtcggctatc tcgtcgacgg
     5281 cttccttggg tcgctggtct cgaccatcgg aattttcctg ccttcgttca tccttgtgct
     5341 gatcgccgcg ccgatcctcg cgcgctatcg cggcaacgct tatgtccagg gcttcgtcaa
     5401 aggcgcctat ggcgccgcga tcggcacaat tctcggcgct tgcatcctcc tcggcaggat
     5461 tgcgatcggc gattggctga ccgccttgat cgggatcgcc tcgctcgccg ccctgttccg
     5521 ttggaaagtg agcaatccgc tgctcatggc ggcgacggca tgcgtcggct tggtcgcctt
     5581 cccggcgatg cagccggatt gggtcatggt caagtaagcg gccgatgaaa aaggagcttt
     5641 ccatgcttcg aacgatccgc ctggtccttc tcgtcgcgtg cggcgcattg acgggcgtgg
     5701 tgttcggcgc cgatcgcgat tggagcaggg tcggagacgc tttggggaaa gccggttcgc
     5761 tgcagcccgg cggcgtctac cgggtggcct tgccgaggac ggaccttaag gtcgccgtcg
     5821 acggcgtcac gctgaaagcc ggttttgcgc tcggcggctg gatcgccttc gagccgatgg
     5881 ggagcgaagc gatgatgatg ggcgatctcg ttctcgccga atccgaaatc aatcccgtca
     5941 tgtcgaagct cctcgtggaa gggctgcaag tcacggcgct gcacaatcac cttttgcgcg
     6001 ccgcgccgcc gattttttat atgcatgtcg ccggcaaagg cgacgccgag caactcgccc
     6061 gcgcggttcg cggcgcgctc gagcaaagca agacgccgct cggctcggcc gcgacaccgt
     6121 cggccgccgg cgcggatctc gggatcgaca gcgccgcgat cgatcgtgcg atcgggttca
     6181 aaggccgcaa caatggcggc gtttatcagt acagcctgcc tcgttccgac agcgtcaagc
     6241 aggacgggat gaagatgccg cccgccatgg gcgtcgccat cgggatcaat ttccagccca
     6301 cgggcgacgg ccgggcggcc gtgaccgggg atttcgtggc gctggcgaac gaagtgaatc
     6361 cattgatcaa ggcgctgcgc gagaacggga tcgaagtcac ggcgatacac aaccacatgc
     6421 tcgacgacga gccgcgggtc tttttcatcc atttctgggc gaacgacgat gcggtgaagc
     6481 tggccaaagg cttgcgagcc gccctcgacg ccgcccatgt cgcgccgaag ggatagacgc
     6541 atcgcctcgc ggcgcaagca ggggccgtga tcggctagac tagactagct tcagcgccgc
     6601 cggtcgaaga gcggaaagcc cagaaacccc acaacgaagc cgccgatatg ggcgagccag
     6661 gcgaccggtc cttcggaaag gccgagggtc tgcgcgcccg cgccgaagat gaaattggcg
     6721 agga
//
LOCUS       DATGJW010000405         4383 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_908407, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000405 DATGJW010000000
VERSION     DATGJW010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4383)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4383)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4383
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_908407"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /gene="tagH"
                     /locus_tag="VKU03_12210"
     CDS             <1..598
                     /gene="tagH"
                     /locus_tag="VKU03_12210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="type VI secretion system-associated FHA domain
                     protein TagH"
                     /protein_id="HLJ72075.1"
                     /translation="PAPAGSDVLSGIARAAGIDPRAFESRDPAAVAEDIGAVLRLLAH
                     HLAQMLASRSETKGLIRSANRTMIRPHENNPLKFTATPEEALAVMFGPPTRNYLNAQA
                     AVERSFADLKAHQIQTFGAMQAALEALFEDLAPEKIDRSVEAERGLGGLVGSRKAKLW
                     DVYAERWRAKAKRSDGRLSEAFMALFAEFYDRLQKKEP"
     gene            677..2014
                     /gene="tssK"
                     /locus_tag="VKU03_12215"
     CDS             677..2014
                     /gene="tssK"
                     /locus_tag="VKU03_12215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssK"
                     /protein_id="HLJ72076.1"
                     /translation="MSWYAKVNWSEGLFLRPHHLQQADRYFENLLESRTRHVSPYPWG
                     FSLLEVDRDLAQQSKFALRRAAGVMPDGTIFDFPADSPAPAPIEAPETAAGQLVWLAM
                     PAKAMNSREVAQDGGDGASRFIAGAETVIDSSSHLRIEEEIDVAYPRLAYEIRKTAKP
                     GYVGLAVARIVEVHDRTIVFDEKFAPPLLVCSAHPTVEGWLDRVIGWINAKLDELARY
                     ASDATAGGGLQSADYLMLQMLNRETPVLKHFRLSRYVHPERLYEELLRIAGELATFVT
                     SERRARDYGAYNHDDLELTFAPVLRDIQDFLSARLDRRAIRLELIERAQNAYISTIKD
                     RSLFRNATLVLEVSARRPLIEIQQQLPHFLKVGPNTKMNEIVHAHLPGVTLVHLPTPP
                     PQIRAISDHVYFYLDRASPLWPEFSVASAIGMHFAGDWPELQMELWAIREDRR"
     gene            2011..3561
                     /gene="tssL"
                     /locus_tag="VKU03_12220"
     CDS             2011..3561
                     /gene="tssL"
                     /locus_tag="VKU03_12220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493497.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system protein TssL, long
                     form"
                     /protein_id="HLJ72077.1"
                     /translation="MSEPDDPFGRRDRTIIRPNPGGRRPGPSQGGAPPASAPNPPVSY
                     PPPQQPPQAPYQPQQPYQPPPQQPSQPPPAAPPSSAGQFNWDGWATSPTPQPANPYLQ
                     PAAGVAPIVPAPASPHIPVDIVSVANNPLMRAASSLLLLLGRLRASLSRAGSGELMDQ
                     VAQAISQFEADARAAGIPNERIAAAKYALAATADDIVQNLPTDDRHLWTKYSMLVRFF
                     GERTGGVRFFQELDRAKQNPALNLDLLEVMHACLCLGFEGVYRSSAGGVGTLQGIRRD
                     IYETIRRVQSKEVEDLSPHWRGLTIALAGSRFRLPVWSAAAAACVILLAVYLVLRNLL
                     SDQAEALALRMSGQPGGEITIARETAVKPPPDPPRRSTQLERIRAALSNEIMAGKLTA
                     DQTATTIFIRIGNLVLFPSGAAKVSESFAPIAAKIAKTLEKEPGGIRIDGYTDTDPIK
                     TVAFPSNWELSEARAKAVAALLRPELSKPERIAVFGRGTDNPIAPNDTPENKAKNRRV
                     EISIPRAD"
     gene            3599..>4383
                     /locus_tag="VKU03_12225"
     CDS             3599..>4383
                     /locus_tag="VKU03_12225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591867.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion protein IcmF/TssM N-terminal
                     domain-containing protein"
                     /protein_id="HLJ72078.1"
                     /translation="MNAMAWIWIALTVISALIFAALVWFAGPIIFIGDAQPFESVWLR
                     LLIIVVLFAIVGATIAYQIVKRQRAAKALEQAMAAPAPEETDAPVLAEKMQDALATLK
                     RSSKSGAGFLYDLPWYLIIGPPGAGKTTALVNSGLRFPLAGDNAARAVQGVGGTRYCD
                     WWFTDEAVLIDTAGRYTTQDSDAKADRKSWLAFLDLLRKNRPRQPINGVLVAISIDDI
                     VRLPPAEVNAHADAIRKRLAELHDELKIDFPVYAIFTKMDLVVG"
BASE COUNT          757 a         1511 c         1385 g          730 t
ORIGIN      
        1 gccggcgcca gccgggtcgg atgtcctttc cggcatcgcg cgcgccgccg gaatcgatcc
       61 gcgggcgttc gaaagccgcg acccggccgc ggtcgccgag gatatcggcg ccgtcttgcg
      121 cttgctggcc catcacctcg cgcaaatgct cgcttcgcgc tccgaaacca agggcctgat
      181 ccgcagcgcc aatcggacga tgatccggcc gcacgagaat aacccgttga aattcaccgc
      241 gacgcccgag gaggcgctcg ccgtcatgtt cgggccgccg acccgcaatt acctcaacgc
      301 ccaggcggcc gtggagcgca gcttcgccga cctcaaggcg catcaaatcc agactttcgg
      361 agcgatgcag gcggcgctgg aagctttgtt cgaggacctc gcgccggaga agatcgaccg
      421 ttcggtcgag gccgagcgcg gccttggcgg cctggtcggg tcccgtaaag caaaattatg
      481 ggacgtctat gcggagcgct ggcgcgccaa ggcgaaacgg agcgacggcc gcctctcgga
      541 ggcgttcatg gcgctgttcg ccgagttcta cgaccggttg cagaaaaagg agccgtagcc
      601 cgcgctctct cgccaaagga gcccggcggc gccaagcggc tttcgtccga ttttagggaa
      661 ctggggtatt ccggccatgt cgtggtacgc aaaggtcaat tggtcggaag ggctgttcct
      721 gcggccgcat cacctgcagc aggccgatcg ctatttcgag aatctgctcg agagccgaac
      781 ccggcatgtt tcgccctatc cgtggggctt ctcgctgctc gaagtcgacc gcgacctcgc
      841 ccagcagagc aagttcgcgc tgcggcgcgc cgccggcgtg atgccggacg gaacgatttt
      901 cgattttcct gccgatagtc ccgcgccggc gccgatcgaa gcgccggaaa cggcggcggg
      961 gcagctcgtc tggctcgcca tgcccgccaa ggcgatgaat tcgcgcgaag tcgcgcaaga
     1021 cggcggcgac ggcgcgtcgc gcttcatcgc tggggccgag acggtgatcg attcgagctc
     1081 gcatttgcgc atcgaggagg agatcgacgt cgcctatccg cggctcgcct acgaaatccg
     1141 caagaccgcc aagcccggct atgtcggcct ggccgtcgcg cgcatcgtcg aggtgcatga
     1201 ccgcacgatc gtgttcgacg agaaattcgc gccgccgctg ctggtctgct cggcccatcc
     1261 gacggtggag ggctggctcg accgcgtgat cggctggatc aacgccaagc tcgacgagct
     1321 cgcgcgctac gcgagcgacg cgacggccgg gggcggcctg caaagcgctg actatctgat
     1381 gctgcaaatg ctgaaccgcg aaacgccggt gctcaagcat ttccgcctgt cgcgctacgt
     1441 gcatccggag cgcctttacg aggagctttt gcgcattgcc ggcgaattgg cgacgttcgt
     1501 caccagcgaa aggcgggcgc gcgactacgg cgcctataat cacgacgatc tcgaactcac
     1561 tttcgcgccg gtcctgcgcg acatccagga tttcctgagc gccaggctcg accgccgcgc
     1621 gatccgactg gagctgatcg agcgcgcgca gaacgcttac atatcgacga tcaaggatcg
     1681 cagcctattc cgcaacgcga cgctggtgtt ggaagtctcg gcgcggcggc cgcttatcga
     1741 aatccagcag caattgccgc atttcctgaa agtcgggccg aacaccaaaa tgaacgagat
     1801 cgtccacgcc catctgccgg gcgtcacgct cgtccatttg ccgacgccgc cgccgcagat
     1861 cagggcgatt tccgaccacg tctatttcta tctcgaccgc gcttcgccgc tctggccgga
     1921 gttcagcgtg gcgagcgcga tcggcatgca tttcgccggc gattggcccg agctgcaaat
     1981 ggagctctgg gcgatcaggg aagataggcg atgagcgaac ccgacgatcc gttcggccgt
     2041 cgcgaccgga ccatcattcg tcccaatccc ggcgggcgcc ggcccggccc gagccagggc
     2101 ggcgcgccgc cggccagcgc gcccaatccg ccagtctcct atccgccgcc acaacagccg
     2161 ccgcaagcgc cgtaccagcc gcaacagcct taccagcctc cgccgcagca accctcccag
     2221 ccgccgccgg cggcgccgcc ttcctcggcc ggccagttca actgggacgg ctgggccacc
     2281 agcccgacgc cgcagccggc caatccctat ctgcagcccg cggccggcgt tgcgccgatc
     2341 gtaccggcgc cggcttcgcc gcatatcccc gtcgacatcg tctcggtcgc gaacaatccc
     2401 ctgatgcgcg cggcctcgtc cttgctgctg ctgctcggcc gcttgcgcgc ctcgctgtcg
     2461 cgcgccggct cgggcgagct gatggaccag gtcgcccagg cgatcagcca attcgaggcg
     2521 gacgcgcgcg ccgcgggaat tcccaacgag cggatcgcgg cggcgaaata cgcgctcgcc
     2581 gccaccgccg acgatatcgt ccagaaccta ccgaccgacg accggcattt atggaccaaa
     2641 tacagcatgc tggtgcgctt tttcggcgag cgcaccggcg gcgtccgctt cttccaggag
     2701 ctcgaccgcg ccaagcaaaa tccggcgctc aatctcgacc ttctcgaagt catgcatgcc
     2761 tgcctgtgct taggcttcga aggcgtgtac cggagttcgg ccggcggcgt cggcacgctg
     2821 cagggcatac gccgcgacat ttacgagacg atcaggcgcg tccaatcgaa ggaggtcgag
     2881 gatctctcgc cgcattggcg cggcttgacc atcgcgctcg ccggcagccg ctttcgcttg
     2941 ccggtatggt cggcggcggc ggccgcttgc gtcatcctcc tcgccgtcta ccttgtcctg
     3001 cgcaatctgc tgagcgacca ggccgaggcg ttggcgctga gaatgagcgg gcagcctggc
     3061 ggcgagatca caatcgcgcg cgagacggcg gtcaagccgc cgcccgatcc gccgcgccgc
     3121 tccacccagc tcgaacgcat ccgcgccgcg ctctccaacg agatcatggc cggcaagctg
     3181 accgccgatc agaccgcgac gacgattttc atccgcatcg gcaatcttgt ccttttcccg
     3241 tcgggagcgg ccaaggtcag cgaatccttt gcgccgatcg ccgccaagat cgccaagacc
     3301 ttggagaagg agccgggcgg cattcgcatc gacggttata ccgacaccga tccgatcaag
     3361 actgttgcgt tcccctcgaa ttgggagctg tcggaggctc gggcgaaagc ggtggcggcg
     3421 ctgttgcggc cggaattgtc gaagccggaa cgcatcgccg tgttcggccg ggggaccgac
     3481 aatccgatcg cgccgaacga tacgccggag aacaaagcca agaacaggcg ggtggaaatc
     3541 tccatcccgc gcgccgattg aggccgccga tgacaagcgc gccggagccg ctctttccat
     3601 gaacgccatg gcctggatct ggatcgcctt gaccgtgatc agcgcgctga ttttcgccgc
     3661 gctcgtctgg ttcgccgggc cgatcatctt catcggcgac gcgcagccct tcgaaagcgt
     3721 gtggctgcgc ttgctgatta ttgtcgtgct tttcgcgatc gtcggcgcca cgatcgccta
     3781 ccagatcgtc aagcgccagc gcgccgccaa ggcgctcgag caagccatgg ccgcgccggc
     3841 gccggaggag accgacgcgc cggtcctcgc cgaaaagatg caggacgcgt tggcgacgct
     3901 gaagcggagc agcaaatccg gcgccggctt cctctacgat ctgccctggt acctaatcat
     3961 cggcccgccc ggcgcgggca agacgacggc tttggtcaat tcgggcttgc gctttccgct
     4021 tgccggcgac aacgccgcgc gcgccgtcca gggcgtcggc ggcacgcgct attgcgactg
     4081 gtggttcacc gacgaggctg tgctgatcga cacggccggc cgctacacga cgcaggattc
     4141 cgacgccaaa gccgatcgca agagctggct cgccttcctc gatctcttgc gcaagaaccg
     4201 gccgcgccag ccgatcaacg gggtgctggt ggcgatcagt atcgacgaca tcgtccgcct
     4261 gccgccggcc gaagtaaacg cccatgccga cgcgatccgc aagcgcctcg ccgaattgca
     4321 cgacgaattg aagatcgatt tcccggtcta cgccattttc accaagatgg acctcgtcgt
     4381 cgg
//
LOCUS       DATGJW010000406         5722 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1427321, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000406 DATGJW010000000
VERSION     DATGJW010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5722)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5722)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5722
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1427321"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..209)
                     /locus_tag="VKU03_12230"
     CDS             complement(<1..209)
                     /locus_tag="VKU03_12230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="RraA family protein"
                     /protein_id="HLJ72079.1"
                     /translation="MKVIEEPPLLTIARAIERPKAAEIEPFRGVPLSFLCDAMGGDGA
                     LDWRIKPICSPSLFGVALTCQCGPA"
     gene            complement(298..1530)
                     /gene="glp"
                     /locus_tag="VKU03_12235"
     CDS             complement(298..1530)
                     /gene="glp"
                     /locus_tag="VKU03_12235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094614.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990586 - divisome complex [Evidence
                     IEA];
                     GO_function: GO:0061599 - molybdopterin molybdotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="gephyrin-like molybdotransferase Glp"
                     /protein_id="HLJ72080.1"
                     /translation="MSASGLISVEEALARILASVTALPPAETVAVEAALGRTLAEDVR
                     ALRTQPPFANSAMDGYALAAADTANPPARLKVVGESAAGRAFQGAIGRGEAVRIFTGA
                     PMPNGADAVLIQENATREGEAVIAHAREAPGRNVRAAGLDFQEGEALLAAGRRLTPRD
                     LALAAAANHARLNVRRRPRIAILATGDELVPPGAARGPAQIVASNNYAVAGVAAAAGG
                     EAHDLGIAADSKQALAQSFARAAEIKADVLVTLGGASVGDYDLVQQALLESGLELGFW
                     RIAMRPGKPLMHGRLGDMKVLGLPGNPVSSVVSAILFLRPLLLALLGDPNAAADVSEA
                     ARLGADLGENDARQDYLRATLSKEDDGWRAAPVASQDSSLVKLLARAQGLIIRKPHAP
                     AAKSGEPCRIIRFDALGL"
     gene            complement(1527..2012)
                     /gene="moaC"
                     /locus_tag="VKU03_12240"
     CDS             complement(1527..2012)
                     /gene="moaC"
                     /locus_tag="VKU03_12240"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007696101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HLJ72081.1"
                     /translation="MTDLTHIGASGEAHMVDVSAKDETERVATAEGRVAMKQETLQFI
                     RQGDAKKGDVFGAARIAGIMAAKRTHELIPLCHPLLLTSVNVEIVPDEALPGLAVRAT
                     AKTRGQTGVEMEALTAVAVACLTIYDMAKAVDRFMRIEGVRLLEKSGGKSGDWRAPAA
                     P"
     gene            complement(2009..2830)
                     /gene="trpC"
                     /locus_tag="VKU03_12245"
     CDS             complement(2009..2830)
                     /gene="trpC"
                     /locus_tag="VKU03_12245"
                     /EC_number="4.1.1.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004425 - indole-3-glycerol-phosphate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006568 - tryptophan metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="indole-3-glycerol phosphate synthase TrpC"
                     /protein_id="HLJ72082.1"
                     /translation="MSDILQKIETYKRREIADAKVRVPRATLEREIQSQSPPRGFVKA
                     LEREVAAGRFALIAEIKKASPSKGLIREHFDPPALAKAYQQGGAACLSVLTDAPSFQG
                     KPEYLGKARNATRLPALRKDFLFDSYQVLEARAWGADCILIIMACVDDECARTLNKTA
                     HDLGLDVLVEVHNERELERALGLEARLIGINNRDLRTFQTSLETAERLAPMIPKDRLI
                     VAESGISKHGDCRRLAEVGIETFLVGESLMRQGDVGAATRALLTGDEVELKKARH"
     gene            complement(2860..3873)
                     /gene="trpD"
                     /locus_tag="VKU03_12250"
     CDS             complement(2860..3873)
                     /gene="trpD"
                     /locus_tag="VKU03_12250"
                     /EC_number="2.4.2.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196946.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004048 - anthranilate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate phosphoribosyltransferase"
                     /protein_id="HLJ72083.1"
                     /translation="MEAFKPLIAKVATGAALTRAEAVSAFDAMLSGEVTPAQMGGFLM
                     ALRTRGETVEEITGAVEAMRAKMLRVHAPADAIDVVGTGGDNSGSYNVSTLAALIVAA
                     CGAPVAKHGNRALSSRSGAADTLGVLGVKVGIPPEAVERCIRETGIGFMMAPTHHAAM
                     RHVGPARVELGTRTIFNLLGPLSNPAGTRRQLIGVFSAAWLEPMAEVLRNLGAERVWI
                     THGADGLDEMTTAAATRIVELAQGVIRSFEITPEEFGLKRAQPADLKGGDAAHNAAAL
                     TAVLEGAKNSYRDIAMLNAAGALVVAEKARNLKEGIEKAGRALDSGAARATLAKLVAV
                     SNA"
     gene            complement(3890..4489)
                     /locus_tag="VKU03_12255"
     CDS             complement(3890..4489)
                     /locus_tag="VKU03_12255"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116347.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046820 - 4-amino-4-deoxychorismate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminodeoxychorismate/anthranilate synthase
                     component II"
                     /protein_id="HLJ72084.1"
                     /translation="MTAVTLIDNYDSFTFNLVHYLGALGAEVSVWRNDAISVDAIMAG
                     KPDAIVLSPGPCTPNEAGICLDLIRKASAATPILGVCLGHQAIGQAFGAKIVRAPLPM
                     HGKLSRISHNGRGVFRGVNGSFQATRYHSLVVDRKTAPPDLEITAETEDGLIMGLSHR
                     AAPVHGVQFHPESILSEHGRIVLRNFLDLAGAFNAARAA"
     gene            4621..5406
                     /locus_tag="VKU03_12260"
     CDS             4621..5406
                     /locus_tag="VKU03_12260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72085.1"
                     /translation="MLYRARPADGIAWVAGASSGIGRGVALELARRGYAVAASARRAD
                     ALRDLAAEASVLNGRIEPAPLDMTDREAVARLAPTLETRLGPIALVFLNAGVSRPDPP
                     GDLGGENFRQTFEVNVLGLANGLNAALNAMRPRGRGQIAFNASIAGYGGLPAPAAYGA
                     SKAAAIHLAESLKFSAGRYGIAIQVVTHGFVRTPLTAENRFKMPFLMDCETAARRICD
                     GFEKAGFEITFPRRLSWAVKAMNLLPYPLYFALAGQFAPKRSH"
     gene            complement(5434..>5722)
                     /locus_tag="VKU03_12265"
     CDS             complement(5434..>5722)
                     /locus_tag="VKU03_12265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72086.1"
                     /translation="QSRSREQNRSADQRRPPPVRNRGFAGWWIIIAIIIIVIIGWSWG
                     GGWNGWSGGARTAQPAHTTAPAGNAAANPNGATAPANAKGGATNGATTTSH"
BASE COUNT          886 a         1891 c         1978 g          967 t
ORIGIN      
        1 tcggccggcc cgcattggca ggtcagcgcg acgccgaaga gcgagggcga gcagatcggc
       61 ttgatccgcc agtcgagcgc gccgtcgccg cccatcgcgt cgcagagaaa gctcaaagga
      121 acgccgcgaa acggctcgat ctcggcggct ttgggccgct cgatcgcgcg ggcgatggtc
      181 agaagcggcg gctcttcgat cactttcaag tcgctcccta aagcggggcg accgcaaagc
      241 ctgcccctga tttttccgat gctaggccaa attcatgccc ggcgcgacgg cggctggtca
      301 aaggcccagc gcatcgaacc ggatgatccg gcagggctcg ccgcttttcg ccgccggcgc
      361 atgcggcttg cggatgatga ggccctgagc gcgggccagc aatttgacga gcgaggaatc
      421 ctggctcgcg accggcgcgg cgcgccagcc gtcgtcctct ttcgacagcg tcgcgcgcaa
      481 ataatcctgt cgcgcgtcgt tctcgccgag atcggcgccg aggcgcgcgg cttcgctcac
      541 atcggcggcg gcgttcggat cgccgagcag agcgagcaga agcgggcgca ggaacaggat
      601 ggcggagacg acggaggaga ccgggttgcc gggcaggccg agcactttca tatcgcccag
      661 ccggccatgc atcagcggct tgcccggccg catggcgatg cgccagaagc cgagctccag
      721 cccggattcg agcagcgcct gctggacaag atcgtaatcg ccgaccgagg cgccgcccag
      781 cgtcacgagg acatccgctt tgatttccgc ggcgcgcgcg aagctttgcg ccagcgcctg
      841 cttggaatcg gcggcgatgc cgagatcgtg agcctcgccg ccggcggccg cggcgacgcc
      901 ggcgaccgcg taattgttcg aggcgacgat ctgggcgggt ccgcgcgccg cgccgggggg
      961 gaccagttcg tcgccagtcg cgagaatggc gatgcgtggg cggcggcgca cgttaagcct
     1021 cgcatggttc gccgcggcgg cgagggcgag gtcgcgcggg gtgaggcggc ggccggcggc
     1081 gagcagggct tcgccctctt gaaagtcgag gcctgctgcg cgcacgttgc ggccaggggc
     1141 ttcgcgcgca tgcgcgatca cggcctcgcc ttctcttgtc gcattttcct ggatcagcac
     1201 ggcgtcggcg ccgttcggca ttggcgcgcc ggtgaagatg cgcaccgcct cgccgcgccc
     1261 gatcgcgcct tgaaaggcgc ggccggcggc ggattcgccg acgactttca gccgcgccgg
     1321 aggattggcc gtatcggcgg cggcaagcgc atagccgtcc atggccgaat tggcgaaggg
     1381 cggctgcgtg cgcagggcgc ggacgtcctc ggccaaggta cggcccagcg ccgcttcgac
     1441 cgcgaccgtc tcggcgggcg gcagcgccgt gaccgaggcg aggatgcggg cgagcgcctc
     1501 ctcgaccgaa atgaggccgg aggcgctcat ggcgccgccg gcgcgcgcca gtcgcccgac
     1561 ttgccgccgc tcttttctag gagccttacg ccttctatgc gcatgaagcg gtcgaccgcc
     1621 ttggccatgt cgtagatcgt caggcaggcg acggcgaccg cggtcagggc ctccatctcg
     1681 acgccggtct ggccgcgcgt cttcgccgtc gcgcgcacgg cgagaccggg cagggcctcg
     1741 tcgggtacga tctcgacatt gacgctggtc agcagcagcg ggtggcagag cgggatcaat
     1801 tcgtgcgtcc gcttcgccgc catgattccg gcgatgcgcg cggcgccgaa cacgtccccc
     1861 tttttggcgt cgccttggcg aatgaactgc agcgtctcct gcttcatcgc gacgcgccct
     1921 tcggcggtcg cgacgcgctc cgtctcatcc ttcgccgaaa catcgaccat atgcgcttcg
     1981 ccggaagcgc cgatatgggt caaatcggtc aatggcgcgc ctttttcagt tcgacttcgt
     2041 cgccggtcag cagcgcgcgc gtcgccgcgc cgacatcgcc ctgccgcatc aacgattcgc
     2101 cgaccaggaa ggtttcgatt ccgacctcgg cgaggcggcg gcaatcgcca tgtttcgaaa
     2161 tgccgctttc ggcgacaatg agccgatcct tgggaatcat cggcgccagt cgctcggcgg
     2221 tttcgagcga tgtctggaag gtgcgcaagt cgcgattgtt gatgccgatc aagcgcgctt
     2281 cgagccccag cgcccgctca agctcgcgtt cgttatggac ttcgaccagc acgtcgaggc
     2341 cgagatcatg ggcggtcttg ttgagcgtgc gcgcgcattc gtcgtcgacg caggccatga
     2401 tgatgaggat gcaatcggcg ccccaggctc gcgcctccag cacctggtag gaatcgaaga
     2461 ggaaatcctt gcgcaaagcc ggcagccggg tggcgttgcg ggctttgccg agatattcgg
     2521 gcttgccttg aaaggacggc gcgtcggtga gcacggaaag acaggcggcg ccgccctgct
     2581 ggtaggcctt ggcgagggcg ggcgggtcga aatgctcgcg gatcaggcct ttggatgggc
     2641 tcgccttctt gatctccgcg atcagcgcga agcggcccgc ggccacttcg cgctccagcg
     2701 ccttgacgaa gccgcgcggc gggctctggc tctggatttc gcgctccagg gtcgcgcgcg
     2761 gcacccgcac cttggcgtcg gcgatctcgc gccgtttgta cgtctcgatc ttttgcagaa
     2821 tgtcggacaa agcggctccg ttgaagtcac ccggtttcgt caggcattcg aaacagcaac
     2881 cagtttggcg agcgtggcgc gcgccgcgcc ggaatcgagc gcccgtccgg ccttctcgat
     2941 cccttcctta agattgcgcg ccttctccgc gacgacaagc gcgcccgccg cgttcagcat
     3001 agcgatgtcg cgataggaat tcttggcgcc ttcgagcacg gcggtcagcg cggcggcgtt
     3061 gtgggcggcg tcgccgcctt tcaaatccgc cggctgcgcc cgcttgaggc cgaactcctc
     3121 cggcgtgatt tcgaaggagc ggatgacgcc ctgggcaagc tcgacgatcc gggtcgcggc
     3181 ggcggtcgtc atctcgtcga ggccgtcggc gccatgggtg atccagaccc gctcggcgcc
     3241 gagattgcgc aacacctcgg ccatcggctc gagccaggcg gccgagaaga cgccgatcaa
     3301 ctgacgtcgc gttccggccg gattggacag cggaccgagc aggttgaaaa tcgtgcgggt
     3361 gccgagttcg acccgcgccg gaccgacatg gcgcatcgcc gcgtgatgcg tcggcgccat
     3421 catgaagccg atgccggtct cgcgaatgca gcgctcgacg gcctcgggcg gaatgccgac
     3481 tttgacgccg agcacgccaa gcgtatcggc cgcgccggaa cgcgaggaga gggcgcggtt
     3541 gccatgcttg gcgaccggcg cgccgcaggc cgcgacaatc agggcggcga gggtcgagac
     3601 attgtacgaa cccgagttgt cgccgccggt gccgaccacg tcgatcgcgt cggccggcgc
     3661 atggacgcgc agcatcttgg cgcgcatcgc ctcgaccgcg ccggtgattt cctcgaccgt
     3721 ctcgccgcgc gtgcgcaagg ccatgagaaa gccgcccatt tgcgccggcg tcacttcgcc
     3781 ggacagcatg gcgtcgaagg ctgagaccgc ctcggcgcgc gtcagcgcgg cgccggtcgc
     3841 gaccttggcg ataagcggct tgaaggcttc cattcccctc tctgattttt caggcggcgc
     3901 gggcggcgtt gaacgcgccg gcaagatcga gaaaattgcg cagcacgatg cggccatgct
     3961 ccgagagaat gctctccggg tggaactgta cgccatgaac cggcgccgcg cgatgcgaga
     4021 gacccatgat caggccgtcc tcggtctcgg cggtgatttc gagatccggc ggcgcggttt
     4081 tgcggtcgac gaccagcgaa tgatagcgcg tcgcctggaa cgagccgttg acgccgcgaa
     4141 acacgccgcg cccgttatgc gaaatgcgcg acagcttgcc atgcatgggc aggggcgcgc
     4201 ggacgatttt ggcgccgaac gcctggccga tcgcctgatg gccgagacag acgccgagga
     4261 tcggcgtggc cgcgctcgcc ttgcggatga ggtcgaggca aatccccgcc tcgttgggcg
     4321 tgcaggggcc gggcgacagc acgatcgcgt cgggctttcc ggccatgatc gcatcgaccg
     4381 aaatcgcgtc gttgcgccag accgacacct cggcgccgag cgcgcccaga taatggacga
     4441 ggttgaaggt gaaactgtcg taattgtcga tcagcgtaac ggcggtcatc acgaagcttt
     4501 cagggcgggg gcccggacga ttaaactctt gcgttgcccc gccggccccg tcaagccgcg
     4561 ccggttcgat gcgggagggc cggcggcgtc cgcaatcgtc ggcgagaatg gaagagccgc
     4621 atgctttatc gagcccgacc ggcggacgga atcgcctggg tcgccggcgc gagttcggga
     4681 atcggccgcg gcgtcgcgct cgaattggcg cggcggggct acgcggtcgc cgcgagcgcg
     4741 cgccgcgccg acgctttgcg tgatttggcc gctgaagctt cggtcctgaa cggacggatc
     4801 gagccggcgc cgctggacat gaccgatcgc gaggcggtgg cgcgactcgc gccgacctta
     4861 gaaacgcgac tcggcccgat cgcgctcgtc ttcctcaatg ccggcgtttc tcgccctgac
     4921 ccgcccggcg atctcggcgg cgaaaatttc cggcagacgt tcgaagtcaa tgtcctgggc
     4981 ttggccaacg gcctcaatgc ggcgctcaac gccatgcggc cgcgcggccg cggccagatc
     5041 gccttcaacg cctccatcgc cggctatggc gggttgccgg cgccggccgc ctacggggcc
     5101 agcaaggcgg cggcgatcca tctggcggaa agcctcaaat tctcagccgg ccgatacggg
     5161 atcgcgattc aggtcgtgac tcatggtttc gtgcgcacgc cgctcaccgc cgaaaaccgc
     5221 ttcaagatgc cgttcctgat ggattgtgaa acggccgccc ggcgcatatg cgatggtttc
     5281 gagaaggccg gtttcgaaat cacttttccg cgccggctgt catgggcggt gaaggcgatg
     5341 aacctactgc cctatccgct ctatttcgcg ctggccgggc aattcgcgcc caagcgaagc
     5401 cattgacccg cgctgcccgg caaacgcgcg ttttcaatgc gatgtcgttg ttgctccgtt
     5461 cgtcgcgccg cctttggcgt tcgccggcgc ggtcgcgccg ttcggattgg cggcggcgtt
     5521 gccggccggc gcggtcgtat gggccggctg ggcggttctt gcgccgccgc tccaaccgtt
     5581 ccaccctccg ccccagctcc agccgatgat cacgatgatg atgatcgcga tgatgatcca
     5641 ccacccggca aagcctctat ttctgaccgg gggcgggcgc cgttgatcgg ccgatcggtt
     5701 ctgttctctg gaccgcgatt ga
//
LOCUS       DATGJW010000407         6121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1525545, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000407 DATGJW010000000
VERSION     DATGJW010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1525545"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(257..1924)
                     /locus_tag="VKU03_12270"
     CDS             complement(257..1924)
                     /locus_tag="VKU03_12270"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HLJ72087.1"
                     /translation="MSEENELVFVALGGLGEIGMNAALYGFGPARRRKWLMVDCGVSF
                     AGDDLPGIDLILPDIAFIEKAKRDLVGIVVTHAHEDHIGALIDLWPRLGAPVYATRFA
                     AGLLEARRLSEPGAPDIPIEIVDQGKRVGIGPFDVEFIPVAHSIPESSALALRTPAGL
                     VVHTGDWKIDATPLVGLPTDEARFKALGDEGVLALICDSTNVLREGESPSEADVAKSL
                     AALVAKAKGRVLVTAFASNVARLRAAAEAGLANGRQVLVMGRAMERVIAVARECGYLS
                     GVPAFLGMDHFDRLPRDKILALATGSQGEPRAAMARIAVDEHPAASLAPGDTAIFSSR
                     AIPGNEKAVSRIVNGLIAQGVEVVTDRTHLVHVSGHPRRAELARLYAWTRPKIAVPAH
                     GEALHLAEHAAFAKAQGVSNVVKARDGEIVLLDADDAGVVDEIATGRLYKDGAIVLEP
                     GDDCVVQRRRLAFAGIVSIAIAVTAKGDLAGDPDVVIAGLPDKTRAGEPIDEIVDGAI
                     FETFDSMPRPRRRDPEVVSGAIERAVRNAVNMAWGKKPNVHVLVVEV"
     gene            complement(1921..2748)
                     /locus_tag="VKU03_12275"
     CDS             complement(1921..2748)
                     /locus_tag="VKU03_12275"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HLJ72088.1"
                     /translation="MSDAETRVGAWPVEQTLALGWRLEHFPTIGSTNDEARKRALQGD
                     RGRLWIVADEQKAGRGRLGRTWVSPPGNLYASALLLDPAPVARGFEIGFVAALALHRA
                     VSDLGGSGFFLKWPNDLIWRGAKVAGLLAEGVTLASGAFACIIGVGVNCAVAPQGAPY
                     PTTSLSAAFGRAVAPAELFGALAPRFAEALAIWRGGEGFAEIRAAWLQVAAGLGAPIR
                     ISGADGGREGVFEALDARGRLLLRRAGRLEIVEAGDVRLLGEPAPAAGEAAGKASRR"
     gene            complement(2745..4178)
                     /gene="nuoN"
                     /locus_tag="VKU03_12280"
     CDS             complement(2745..4178)
                     /gene="nuoN"
                     /locus_tag="VKU03_12280"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoN"
                     /protein_id="HLJ72089.1"
                     /translation="MIATAFSLPTALPEFILAAGVLALVLLGALRGERSVWLVTEISV
                     ALLGAALVVLLADRGGVSVTFYGAFTDDPFSRFMKALALIGSLTTLLLSIEFMRRAKI
                     AGFEFPILVLLSTLGMMVLISASDFIALYLGFEMMSLALYVVAAYHRDNTRATEAGLK
                     YFVLGALSSGMLLYGLSLVYGFAGTVSFAGVAHAVQGHASIGVVFGLVFLLAGLAFKI
                     SAVPFHMWTPDVYEGAPTPVTAFFASAPKMAAMAVLVRVVITAFPGVTGEWRQIIIFI
                     SIASMALGSFAAIGQTNFKRLMAYSSIGHMGFALVGLAAGTPDGVHGVIIYMAIYLVM
                     TLGTFAAILSMQRNGRYIEDIADLAGLARTNGPMAFFLGAMMFSLAGIPPLGGFFAKF
                     YVFAAAIQANLFALAVIGVLCSVVAAFYYLRIVKIMYFDEPAPAFDHAGLAVRIVLAL
                     STALVIFYSIVPGPLTSAAMAAAKSLF"
     gene            complement(4191..5693)
                     /locus_tag="VKU03_12285"
     CDS             complement(4191..5693)
                     /locus_tag="VKU03_12285"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018042028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HLJ72090.1"
                     /translation="MFGFGVLSGLLILPLIGAAAILFLRGDDPATVNNIRWVAFWTTI
                     ATFILSVVAWYDFNPAESGFQLVEQKIWFSSAISYKVGVDGLSMPFVLLTTFLMPLCI
                     AASWVSIERRVAEYMIAFLVLETLMIGVFVALDLVLFYLFFEGGLIPMFLIIGVWGGK
                     RRVYAAFKFFLYTLLGSLLMLLAIMAMYWTAKTTDIAVLLHTNFPAGMQKWLWLAFFA
                     SFAVKMPMWPVHTWLPDAHVEAPTAGSVILAGILLKMGGYGFIRFSLPMFPDASQYFA
                     PLIYALSIVAIIYTSLVALVQEDMKKLIAYSSVAHMGFVTMGLFAMTPQGVQGAIFLM
                     ISHGLVSGALFLCVGVIYDRMHTRDIAAYGGLVQRMPRYAVALMVFTLANVGLPGTSG
                     FIGEFLTLLGTFPVNTWVALFATTGVILSAAYALYLYRRVIFGVIEKPNLMAIADLST
                     REVAILAPLVILTIYYGVHPQPVIDASAASVDALLKGVGQALAVTKTAGL"
     gene            complement(5694..>6121)
                     /locus_tag="VKU03_12290"
     CDS             complement(5694..>6121)
                     /locus_tag="VKU03_12290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011927053.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HLJ72091.1"
                     /translation="KNNHILENMESAPWLVSVSPTLAMLIGLAVAYHMYIVAPDSPRR
                     MAEANPILYRFLLNKWYFDELYDLIFVRPAFWIGRLFWKGGDGFVIDGFGPDGVSARV
                     LDVTRNVVRIQSGYVYHYAFAMLIGVAALATWYLFGGIR"
BASE COUNT         1275 a         1948 c         2035 g          863 t
ORIGIN      
        1 atcgcgacgt gattcaagcg acctatcatc gggcctccaa agtacgattt tattcgaacg
       61 aatgaccagc gccggcgctt gccctcatcc ggcccttcgg gccaccttct cccgcaagcg
      121 ggagaaggga ttccccgcgc cttaggaaat ccgcgaaagt cgatgaaagc cgcagtgcgc
      181 cccctctccc gcttgcggga gagggttggg gtgagggtaa gccgccgcgt ttgtcgatca
      241 atgcgatgaa gcccgcctat acctcgacga ccaggacatg gacgttcggc tttttgcccc
      301 aagccatgtt cacggcgttg cgcaccgcgc gctcgatggc gccggaaacg acttcggggt
      361 cgcggcgccg gggccgcggc atggaatcga aggtttcgaa aatggcgccg tcgacgatct
      421 cgtcgatcgg ctcgccggcg cgagtcttgt cgggcaggcc ggcgatcacg acatccggat
      481 cgccggcgag atcgcccttg gcggtgacgg cgatggcgat cgagacgatt ccggcgaaag
      541 ccaggcggcg gcgctgaaca acacaatcgt cgcccggctc cagcacgatt gcgccgtcct
      601 tgtaaaggcg accggtcgcg atctcgtcga cgacgccagc gtcatcggca tcgagcagga
      661 cgatctcgcc gtcgcgcgcc ttcacgacat tgctcacgcc ctgggctttc gcgaaggcgg
      721 cgtgctcggc gagatgcagc gcttcgccat gcgccggcac ggcgattttc ggccgcgtcc
      781 aggcgtagag ccgcgctagc tcggcgcggc gcggatggcc cgagacatgg acgagatgcg
      841 tgcggtcggt caccacctcg acgccttgcg cgatcaggcc gttgacgatc cggctcaccg
      901 ccttctcgtt gcccggtatg gcgcgcgacg aaaagatcgc cgtatcgccc ggcgccagcg
      961 aggcggcggg atgctcgtcg acggcgatgc gcgccatcgc ggcgcgtggc tcgccttggc
     1021 tgcccgtcgc caaagccaga atcttgtcgc gcggcaggcg atcgaaatga tccatgccga
     1081 ggaaggccgg cacgccggaa agatagccgc attcgcgcgc caccgcgatg acccgctcca
     1141 tcgcccgccc catcaccagc acctggcggc cattggccaa accggcttcc gccgcggcgc
     1201 gcaggcgcgc gacattcgag gcgaaagccg tgaccaggac gcggcccttg gccttggcga
     1261 caagcgcggc gaggcttttg gcgacgtcgg cttccgacgg gctctcgccc tcgcgcagca
     1321 cgttggtcga atcgcagatc aaggcgagga cgccttcgtc gcccagggct ttgaaacggg
     1381 cctcgtcggt cggcaggccg accagcggcg tcgcgtcgat cttccagtcg ccggtatgga
     1441 cgacgaggcc ggccggcgta cgcagcgcca gcgccgagct ttcagggatc gaatgggcga
     1501 cgggaatgaa ttccacgtcg aacggaccga ttccgacccg tttgccctgg tcgacgatct
     1561 cgatcggaat gtccggcgcg cccggctcgg acaggcggcg cgcctccagc aatcccgcgg
     1621 cgaagcgcgt cgcatagacc ggcgcgccga ggcgcggcca gagatcgatc agcgcgccga
     1681 tatggtcctc atgcgcatgg gtgacgacga tgccgacgag atcgcgcttg gccttctcga
     1741 tgaaggcgat gtcaggcagg atcaagtcga tcccgggcag gtcgtcgccg gcgaaggaga
     1801 cgccgcaatc gaccatcagc catttgcgcc ggcgggcggg gccgaagccg taaagcgcgg
     1861 cgttcatgcc gatctcgccg agcccgccca gggcgacgaa aaccagttcg ttctcctcgc
     1921 tcatcggcgc gacgctttcc ctgccgcctc gcccgcggcc ggagccggct cgccgagcag
     1981 tctcacatcg ccggcctcga cgatttcgag acggccggcg cggcgcagca acaggcgtcc
     2041 acgggcgtca agcgcctcga aaacgccctc gcggccgcca tccgcgccgg aaatccggat
     2101 cggcgcgccg aggccggctg cgacttgcag ccaagccgcg cgaatctcgg cgaagccctc
     2161 gccgcctcgc catatcgcca gggcctcggc gaaacgcggc gccagcgcgc cgaaaagctc
     2221 ggccggcgcg acggcgcggc cgaaagcggc cgaaagcgat gtcgtcggat aaggcgcgcc
     2281 ttgcggcgcg accgcgcaat tgacgccgac gccgatgatg caggcgaagg cgccgctggc
     2341 aagcgtaacg ccttcggcca gcagcccggc gaccttggcg ccgcgccaga tcaaatcatt
     2401 cggccatttc aggaaaaagc cgctcccgcc gagatcgctc accgcccgat gcaaggcgag
     2461 cgccgcgacg aagccgattt cgaagcccct ggcgaccggc gcgggatcga gaagcagcgc
     2521 gctcgcgtaa agattgcccg gcggcgagac ccaggtccgg ccgagccggc ccctgcccgc
     2581 cttctgctcg tcggcgacga tccacagacg gccgcgatcg ccttgcagcg cccgctttcg
     2641 cgcctcgtca ttggtcgagc cgatcgtcgg aaaatgttcg agccgccaac cgagcgcgag
     2701 cgtctgctcg accggccacg ccccgactcg cgtttcggcg tcgctcaaaa cagcgacttc
     2761 gccgcggcca tggcggcgct ggtcagcggg cccggcacga tcgaatagaa aatcaccaga
     2821 gccgtcgaca gcgccaggac gatgcgcacg gcgaggccgg catggtcgaa agccggggcc
     2881 ggctcatcga aatacatgat cttgacgatc ctcaaatagt agaacgccgc caccacgctg
     2941 caaagaacgc cgatcaccgc cagcgcgaac agattggcct ggatcgccgc cgcaaagaca
     3001 tagaatttgg cgaagaagcc gccaagcggc ggaatgccgg ccagcgagaa catcatcgcg
     3061 ccgagaaaga aggccatcgg cccattggtc cgggcaaggc cggcgaggtc ggcgatgtcc
     3121 tcgatgtagc ggccgttgcg ctgcatggag agaatggcgg cgaaggtgcc gagcgtcatg
     3181 accaggtaga tcgccatgta gatgatgacg ccatgcacgc cgtcaggcgt gccggcggcg
     3241 aggccgacca gggcgaagcc catatggccg atcgaggaat aggccatcag ccgcttgaaa
     3301 ttggtctggc cgatcgcggc gaaggagccg agcgccatcg aggcgatcga gatgaagatg
     3361 atgatctgcc gccattcgcc ggtgacgccg ggaaaggcgg tgatgacgac gcgaaccagc
     3421 accgccatcg ccgccatctt cggggccgag gcgaagaaag cggtcaccgg cgtcggcgcg
     3481 ccctcataga cgtctggcgt ccacatatgg aacgggacgg cggaaatctt gaaggcgagg
     3541 ccggccagca agaagacgag gccgaagacg acgccgatgc tggcatggcc ctgcaccgca
     3601 tgggcgacgc cggcgaagga gacggtgccg gcgaagccgt aaaccagcga caggccgtaa
     3661 agcagcatgc cggacgacag cgcgccgagc acgaaatatt tcaggcctgc ctcggtcgcg
     3721 cgcgtattgt cacgatgata cgcagcgacg acatagagcg ccaggctcat catctcgaag
     3781 ccgagataga gcgcgatgaa atcgctggcc gagatcagca ccatcatgcc gagcgtcgac
     3841 aaaagaacca ggatcgggaa ttcgaagccg gcgattttgg cgcggcgcat gaactcgatc
     3901 gacaagagca gcgtcgtcag cgagccgatc agcgccagcg ccttcatgaa ccggctaaac
     3961 ggatcgtcgg tgaacgcgcc gtagaaggtc acgctcacgc cgccgcgatc ggcaagcagg
     4021 acgacgagcg cggcgccgag cagcgcgacg gagatctcgg tgacgagcca aaccgaccgc
     4081 tcgcccctta aggcgccgag caggaccaaa gccaggacgc cggcggcgag aatgaattcc
     4141 ggcagcgcgg tcggcaggga gaaggcagtg gcgatcacgt cgtcgtttcc tcaaagtccg
     4201 gcggtcttgg tgacggccag cgcctgcccg acgcccttga gcagggcgtc gaccgaggcc
     4261 gcgctggcgt cgatgaccgg ctgcggatgc acgccatagt aaatcgtcag gatgacgagc
     4321 ggcgccagaa tggcgacttc ccgagtcgac aggtcggcga tcgccatcag attgggcttc
     4381 tcgatgacgc cgaaaatgac ccggcgatag agatagagcg cgtaggccgc cgacaggatg
     4441 acgccggtgg tcgcgaacag cgcgacccag gtgttgaccg ggaaagtgcc caaaagggtg
     4501 aggaattcgc cgatgaagcc ggacgtgccc ggcagaccga cattggccag ggtgaagacc
     4561 atcagggcga ccgcatagcg cggcatgcgc tggaccagcc cgccataggc ggcgatgtcg
     4621 cgggtatgca tgcggtcgta gatgacgccg acgcaaagga acagagcgcc cgagacgagg
     4681 ccgtgcgaga tcatcaggaa gatcgcgccc tgcacgcctt gcggcgtcat cgcaaagagc
     4741 cccatcgtca cgaatcccat atgggccacg gacgaatagg cgatcagctt cttcatgtcc
     4801 tcctgcacga gagcgacgag cgaagtgtag atgatcgcga cgatcgacag cgcatagatc
     4861 aacggcgcaa aatattgcga ggcgtccggg aacatcggca gcgagaagcg gatgaagccg
     4921 tagccgccca tcttcaggag aatgcccgcc aggatcaccg agcccgccgt gggcgcctcg
     4981 acatgcgcgt cgggcagcca ggtatggacc ggccacatcg gcattttcac cgcgaacgag
     5041 gcgaagaagg cgagccacag ccatttctgc atgcccgcgg ggaagttggt gtggagcaga
     5101 acggcgatgt cggtggtctt cgccgtccaa tacatcgcca tgatggcgag cagcatcagc
     5161 agcgagccga gcagcgtgta gaggaagaat ttgaacgccg cgtagacgcg gcgcttgccg
     5221 ccccagacgc cgatgatcag gaacataggg atgaggccgc cctcgaagaa aagatagaag
     5281 aggacgaggt cgagcgcgac gaagacgccg atcatcagcg tttcgagaac cagaaacgcg
     5341 atcatgtatt cggcgacgcg ccgctcgatc gagacccagg acgccgcgat gcagagcggc
     5401 atcaggaatg tggtgaggag aacgaacggc atcgacaggc cgtcgacgcc gaccttgtag
     5461 gaaatcgcgc tcgagaacca gattttctgc tcgacgagtt ggaagccgga ttcagccgga
     5521 ttgaaatcgt accaggcgac caccgacaga atgaaagtcg cgatcgtcgt ccagaaagcg
     5581 acccagcgga tattgttgac ggtcgccgga tcgtcgccgc gcaagaacag gatcgccgcc
     5641 gcgccgatca gcggcaggat caaaagtccc gagaggacgc caaagccgaa catttagcgg
     5701 atccccccga aaagatacca ggtcgccaag gccgcgacgc cgatcagcat ggcgaaggcg
     5761 tagtgataga catagccgct ctgaatgcgg acgacattgc gcgtcacgtc gagaacgcgg
     5821 gccgaaacgc cgtcagggcc aaagccgtcg atgacgaagc cgtcgccgcc cttccagaac
     5881 aggcggccga tccaaaaggc cggcctgacg aaaatcaggt catagagctc gtcgaaatac
     5941 catttgttga gcaggaaacg atagaggatc ggattggcct cggccatccg gcgcggcgaa
     6001 tcgggcgcga cgatatacat gtgataggcg acggcgaggc cgatcagcat cgccaaggtc
     6061 ggcgatacgc tgaccagcca aggcgcgctt tccatgtttt cgagaatgtg gttgttcttg
     6121 g
//
LOCUS       DATGJW010000408         3582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_699724, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000408 DATGJW010000000
VERSION     DATGJW010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3582
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_699724"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..661)
                     /locus_tag="VKU03_12295"
     CDS             complement(<1..661)
                     /locus_tag="VKU03_12295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007595219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HLJ72092.1"
                     /translation="MLPFLYRRAQDETAALQAALAAAVRSEPPTSAKTQFLAGGTTLL
                     DLMKLDVMHPSELIDINRLAGLDRIEMTPNGLRLGALAKMADAAGHPLLADYPVVAQS
                     LALAASAQLRNMATLGGNVLQRTRCTYFRDPSWKACNKRAPGSGCAALDGCNRAHAVL
                     GVSDHCIATYPGDFAQALIALDASVEIRRRDGDRIIAFADLHRQPGDRPDIETVLAPD
                     EL"
     gene            complement(661..1254)
                     /locus_tag="VKU03_12300"
     CDS             complement(661..1254)
                     /locus_tag="VKU03_12300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319114.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S)-binding protein"
                     /protein_id="HLJ72093.1"
                     /translation="MPTAFNRRTFLGAAVSSAALPAAVEAAAQPQRSSPPDRQSLQTV
                     SLIVNGQQHALALDARTTLLDALREHIGLTGTKKGCDHGQCGACTVHIDGRRVLSCLT
                     LAAMADGAQVTTIEGLAKPDGSLHPMQQAFIDKDAFQCGYCTPGQIMSAIACVEEGHA
                     GSDAEIREYMSGNLCRCAAYPNIVAAINQAKSEMKRA"
     gene            complement(1354..1983)
                     /locus_tag="VKU03_12305"
     CDS             complement(1354..1983)
                     /locus_tag="VKU03_12305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008835222.1"
                     /note="This protein belongs to the uracil DNA glycosylase
                     superfamily, members of which act in excision repair of
                     DNA. However, it belongs more specifically to UdgX branch,
                     whose founding member was found to bind uracil in DNA
                     (where it does not belong), without cleaving it, appears
                     to promote DNA repair by a pathway involving RecA, rather
                     than base excision; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UdgX family uracil-DNA binding protein"
                     /protein_id="HLJ72094.1"
                     /translation="MRAALVAEGRLAELRQDAAHCRACPLWAGATQTVFGEGPADASI
                     VFVGEQPGDQEDRLGRPFVGPAGRLFDRALDEAGLDRDKVYVTNAVKHFKFTQRGKRR
                     LHKRPNAGEIRVCRRWLFEEVQALAPRLIVTLGATAAQSLAEKPLPIGANRGKVIELA
                     SGLRIFATIHPSYLLRVPDEAARAAEYDHFVEDLRAVRALSGKRKAAAR"
     gene            2112..2675
                     /locus_tag="VKU03_12310"
     CDS             2112..2675
                     /locus_tag="VKU03_12310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type 1 glutamine amidotransferase
                     domain-containing protein"
                     /protein_id="HLJ72095.1"
                     /translation="MPSIRDARILILATDGFEQLELTTPRDELRKAGAHVDVASPKGG
                     PIRGWNKTDWGESVPSDLAIASAKPEDYDALVLPGGVINPDKLRIDEAAMKIVRSFME
                     SGKVVAAICHGPWLLVQADAVRGLNMTSYKSIRKDVENAGGKWVDKEVVTDKGVVTSR
                     SPDDLKAFVPKIVEEIEEGRHQRRSAA"
     gene            complement(2719..>3582)
                     /locus_tag="VKU03_12315"
     CDS             complement(2719..>3582)
                     /locus_tag="VKU03_12315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HLJ72096.1"
                     /translation="IRAYLNTLKPVHNPVAVNTLPFPFNIRGAMLVWNMLYFKKGRLQ
                     ADHAKSAEWNRGAYLVEGPGHCGACHTPKTMIGGDIADQKLQGYRLQGWFAPNITDDP
                     ARGLGKWSIADIVSYLKSGHNALTAAAGIMAEEISLSSSKMTGADLKAIAVYLKDQPG
                     SSEAPAPLASSSPAMKAGAAIYQDVCSACHAPDGKGVPNLFPSLAASSNVRSADPTSL
                     IRVVLEGARSVATPSEPTGPGMPAFGRQLNDSEIAAVLTFVRNAWGSGAAAVSEKAVR
                     DQRKKMAAKNY"
BASE COUNT          611 a         1148 c         1200 g          623 t
ORIGIN      
        1 tcaactcgtc aggcgccaga accgtctcga tgtcgggccg gtcgccgggc tgacgatgaa
       61 gatcggcgaa agcaatgatc cggtcgccgt cccgccgccg gatttcgacg ctcgcgtcga
      121 gcgcgatcag agcctgcgcg aaatcgccgg gatacgtggc gatgcaatga tcgctgaccc
      181 cgagcacggc gtgggcgcgg ttgcaaccgt cgagcgccgc gcagcccgag ccaggcgcgc
      241 gcttgttgca ggccttccag gacggatcgc gaaaatacgt acagcgcgtg cgctgcagga
      301 cgttgccgcc caatgtcgcc atattgcgca attgcgcgct cgcggcgagc gccaggctct
      361 gcgcgacaac cggatagtcc gcaaggagcg gatggcccgc cgcatccgcc atcttggcca
      421 acgcgccgag ccggaggccg tttggcgtca tttcgatccg gtcgagaccg gcgaggcggt
      481 tgatgtcgat cagctcgctc ggatgcatga cgtcgagctt catcagatcg agcagcgtcg
      541 ttccgccggc gaggaattgg gtcttggcgg aagtcggcgg ttcgcttcgg accgcggcgg
      601 cgagagccgc ttgcagcgcc gccgtctcgt cttgcgcgcg acggtaaagg aatggaagca
      661 tcacgccctc ttcatctccg acttggcctg attgatcgcc gcgacgatgt ttggataggc
      721 ggcgcagcgg caaaggttgc cgctcatata ttcgcggatc tcggcgtcgc tgccggcatg
      781 gccttcctca acgcaggcga tcgccgacat gatctggccg ggcgtgcaat agccgcattg
      841 aaacgcgtct ttgtcgatga aggcctgttg catcgggtga agcgagccgt cgggcttggc
      901 cagtccttcg atcgtcgtca cctgcgcgcc gtccgccatg gcggcgagag tgaggcagga
      961 aaggacgcgc cgtccgtcaa tatgaaccgt gcaggcgccg cattggccgt gatcgcatcc
     1021 cttcttcgtc ccggtcaggc cgatatgctc gcgaagcgcg tcgagcagcg tcgtgcgcgc
     1081 atcgagcgcg agcgcatgct gttgcccgtt gacgatgagc gaaacggtct gaagggattg
     1141 gcggtccggc ggactcgacc gctgcggctg cgcggccgcc tctacggccg ctggaagggc
     1201 ggcgctcgac acggctgctc ccaaaaaagt tcgccggttg aaagctgtcg gcatggacga
     1261 tgtctccccg cgcctcgctc atgcgccaag cgaggccaac gtcgctcgcg gattttcgtt
     1321 cctccgcgcc ggcgcgcgcg gcgcggcgcc gtctcagcgc gccgccgcct ttctcttgcc
     1381 gctcaacgcg cgcacggcgc gcaaatcctc cacgaaatgg tcgtattcgg cggcccttgc
     1441 cgcctcgtcg ggcacacgca agagatagga cgggtggatc gtcgcaaaaa tgcgcaagcc
     1501 gctggcgagt tcgatcacct tgccgcgatt ggcgccgatc ggcagcggct tttcggcgag
     1561 gctctgcgcc gcggtcgcgc cgagcgttac gatcagccgc ggcgcgagcg cctggacttc
     1621 ctcgaacagc cagcgccggc agaccctgat ctcgccggcg ttcggccgct tgtgcagccg
     1681 ccgcttgccg cgttgcgtga atttgaaatg tttcaccgcg ttggtgacat agaccttgtc
     1741 gcggtcgagc ccggcctcgt cgagcgcgcg atcgaaaagc ctgccggccg gtccgacaaa
     1801 gggccggccg agccggtctt cctgatcgcc cggctgctcg ccgacaaaga cgatcgacgc
     1861 gtcggcgggg ccttccccga agaccgtctg ggtcgcgccc gcccagaggg ggcaggcgcg
     1921 gcaatgcgcg gcgtcttgcc gcaattctgc gagtcttccc tcggcgacca aggcggcgcg
     1981 catagctgag gtcctatccg gcccatcaag cgggcctgac gggggtctgt tccactcgcc
     2041 gcaattcgcg gaactttgcc gaataaccgc cgttggccgg acggggcgat ccgaaccaag
     2101 ggagacttaa gatgccgagc attcgcgacg cgcgcatcct catcctcgcc actgacggtt
     2161 tcgagcaatt ggagttgacc acgccgcgag acgaattgcg caaagccggc gcgcacgttg
     2221 acgtcgcctc gccgaaaggc ggcccgatcc gcggctggaa taagacggat tggggcgaga
     2281 gcgtcccgtc cgacctggcg atcgccagcg ccaagccgga agactacgac gcgctcgtcc
     2341 tgccgggcgg ggtgataaat cccgacaagc tgcgcatcga cgaagcggcg atgaaaatcg
     2401 tccgctcctt catggaatcc ggcaaagtcg tcgccgcgat ctgtcacggg ccctggctgc
     2461 tcgtgcaagc cgacgcggtg cgcggactga acatgacgtc gtacaaatcg atccgcaagg
     2521 atgtcgagaa tgccggcggc aaatgggtcg ataaggaggt cgtgacggat aagggcgtcg
     2581 tcacgagccg cagccccgac gacctgaagg ccttcgttcc gaaaatcgtc gaggagatcg
     2641 aagaagggcg tcaccagcgc cgcagcgccg cctaacagcc gtgaccggcc gcgctcacgg
     2701 cggccggtca cgattgtctc aatagttttt cgccgccatt ttcttgcgct ggtcgcgcac
     2761 cgccttttcg ctcaccgccg ccgcgcccga gccccaggcg ttgcgcacga aagtcaacac
     2821 ggcggcgatc tcggaatcgt tgagctgtcg gccaaaagca ggcatgcccg gcccggtcgg
     2881 ctcgctcggc gtcgcgacgc tgcgcgcccc ttcgagcacg acgcggataa ggctcgtcgg
     2941 atcggccgag cgcacgttgg aggaggcggc gagcgacggg aagagattgg gaacgccctt
     3001 gccgtccggc gcatggcagg ccgagcaaac gtcctgatag atcgcggcgc cagccttcat
     3061 ggcgggcgag gacgatgcga gaggcgccgg cgcctcgctc gatcccggtt gatccttgag
     3121 atagacggcg atcgccttga gatcggcgcc ggtcattttc gaactggaga gcgaaatttc
     3181 ctccgccatg attccggccg cggcggtcaa tgcattgtgg cctgatttca gataggagac
     3241 gatgtcggca atgctccatt tgccgaggcc gcgcgccgga tcgtcggtga tgttcggcgc
     3301 gaaccagcct tgcagccgat agccttgcag cttctgatcg gcgatatcgc cgccgatcat
     3361 ggttttcggc gtgtggcagg cgccgcaatg gccgggccct tcgacgagat aggcgccgcg
     3421 attccactcg gcggacttgg cgtggtcggc ctggagccgt cctttcttga aatagagcat
     3481 gttccagacc agcatcgccc cccggatatt gaatgggaag ggcagcgtgt tgacggcgac
     3541 cgggttatgg accggcttca gcgtgttgag ataggcgcgg at
//
LOCUS       DATGJW010000409         4150 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_806121, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000409 DATGJW010000000
VERSION     DATGJW010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4150)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4150)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4150
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_806121"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1922)
                     /gene="recG"
                     /locus_tag="VKU03_12320"
     CDS             complement(<1..1922)
                     /gene="recG"
                     /locus_tag="VKU03_12320"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent DNA helicase RecG"
                     /protein_id="HLJ72097.1"
                     /translation="MRPALLDPLFAPVATLPGIGPKNAKLFDRLLGRDGRGALLVDVL
                     FHLPYAGLDRRSRPKIRDAVWGQIATIEVKVAEHRPPPSPRSKAPFKVLVEDETGDVE
                     LVFFLANTDWIKKSLPVGATRWVSGKLEIWDGHLQMVHPDRVMDAEALAKLPPVEPVY
                     GLTEGLYPRVVARAAQGAMQRLPAMPEWIDAATLRRLGAPSFREALAAMHSPQQPADI
                     DPAGPAATRLGFDELFANQVALLLVRARMRALKGRSSASEGAIARKIEAALPFRLTGA
                     QAKALAEIRADIASDKRMLRLLQGDVGSGKTVVALLAMAGIVEAGRQAALMAPTEILA
                     RQHFERMQPLAAGAGLRIALITGRDKAAERNRALAALAAGEIDIAVGTHALFQESVAF
                     RDLGLAVVDEQHRFGVHQRLALAGKGEDADLLVMTATPIPRTLVLTYFGDMDVSALTE
                     KPPGRTPIDTRAMPLDRLSDVVAAVGRAIKNGARVYWVCPVIEESEALDVAAAEERAA
                     NLREFFGSAVGLAHGRMKGADKDAAMEAFSQGRTKLLVATTVIEVGVDVPEATVMVIE
                     HAERFGLAQLHQLRGRVGRGAGRSTCLLLYKAPLGDAARARLEILRETEDGFRIAEED
                     LRLRGEGDVLGARQAGA"
     gene            2045..2263
                     /locus_tag="VKU03_12325"
     CDS             2045..2263
                     /locus_tag="VKU03_12325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017967432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72098.1"
                     /translation="MAHASKTHSGAGAKGKSAGTGAMTDLPKDKIEENMALSNRDKAR
                     HPKGRGLDSKDVQTKQLQDHAGNRLDDE"
     gene            complement(2297..3295)
                     /locus_tag="VKU03_12330"
     CDS             complement(2297..3295)
                     /locus_tag="VKU03_12330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019863150.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ72099.1"
                     /translation="MDDFTDRLGRDPDEAAAGRSAASPGVQFKLSHLRMIVALEEHEM
                     VSAAAQAINISQPAASRMIAEMEALLGAPLCERLARGVRLTPLGQSLARHARSVLLQL
                     AQAEREISDLRDGRAGSAALGAVTGPAIDLAAPAIAKLRAIHPKIELTIKIDASNVLA
                     RDLLAARLDFIIARVPDDLNPRLFDCYTIGVEEACLIVRRGHPLTGRGPVSVKDLGAY
                     EWVVQPRGTPLRKTIETMFLAENMAMPERLSNTTSLLLTMIMVAKSNAIAPISVEAAK
                     FMAQSAVGPVEVLPTQRLIVVQPYSLIAVRNRALSPAAQSVYDFIREEAIGRVSRA"
     gene            3459..>4150
                     /locus_tag="VKU03_12335"
     CDS             3459..>4150
                     /locus_tag="VKU03_12335"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72100.1"
                     /translation="MSAATAESASFANIVRLAGVQKYFGAVHALRNINLAVGRNEIVG
                     LIGDNGAGKSTLIKTITGVHKPTSGRIFVRDKEIDLDAYSVGKAHDLSIETVYQDKSL
                     GEKQPLWRNFFVGRQITNRFGFIDVKREKEVANDILINTIGFRGVGISVDSTVQLLSG
                     GERQGISIGRAMHFNSDLIILDEPTAALGVKEVRKVLEFVRRIKESGRASIYIEHNLA
                     HVHEVADRLVVL"
BASE COUNT          725 a         1375 c         1374 g          676 t
ORIGIN      
        1 gcgccggctt ggcgcgcgcc gagcacatcg ccttcgccgc gcaggcgcaa gtcttcttcg
       61 gcgatgcgga aaccgtcttc ggtctcgcgg aggatttcca gccgcgcccg cgcggcgtcg
      121 ccgagcggcg ccttgtagag caggaggcag gtcgagcggc cggcgccgcg accgaccctg
      181 cctcgcaatt gatgcaattg cgccaggccg aaacgctcgg cgtgctcgat caccatcacc
      241 gtcgcctccg gcacgtcgac gccgacctcg atcacggtcg tcgcgacgag cagcttggtc
      301 cgtccttgcg aaaacgcctc catcgcggcg tccttgtcgg cgcctttcat gcggccatga
      361 gcgaggccga cggccgagcc gaagaattcg cgcaaattgg cggcgcgctc ctcggcggcg
      421 gcgacgtcga gcgcctcgct ctcctcgatc accggacaga cccaatagac ccgtgcgccg
      481 ttcttgatcg cccggccgac cgccgcgacg acgtcgctca accggtcgag cggcatggcg
      541 cgcgtatcga tcggcgtgcg gccgggcggc ttttcggtca aagccgacac gtccatgtcg
      601 ccgaaatagg tcagaaccag cgtgcgcggg atcggcgtcg ccgtcatgac caggagatcg
      661 gcgtcttcgc ccttgccggc gagcgccagc cgctgatgca cgccgaagcg atgctgctcg
      721 tcgacgacgg cgaggccgag atcgcgaaag gcgacgctct cctggaacag ggcgtgcgtg
      781 ccgaccgcga tgtcgatctc gccggcggcg agcgcagcaa gggcgcggtt gcgctcagcc
      841 gccttgtccc tgccggtgat caaggcgatg cgcagcccgg cgcccgcggc gagcggctgc
      901 atgcgctcga aatgctggcg cgccaggatt tccgtcggcg ccatcaaggc cgcttgccgc
      961 ccggcttcga cgattccggc catcgccaaa agcgcgacca cggtcttgcc cgagccgaca
     1021 tcgccctgca gcagccgcag catgcgcttg tccgaggcga tgtcggcgcg gatttcggcc
     1081 agcgctttcg cttgcgcgcc ggtcagccga aagggcaggg ccgcttcgat cttccgcgca
     1141 atcgctccct cgctcgcgct gcttcgtccc ttcagcgcgc gcatgcgcgc gcggaccagc
     1201 aacagcgcga cctggttggc gaagagttcg tcgaagccga gccgcgtcgc cgccgggccg
     1261 gccggatcga tgtcggccgg ctgttgcggc gaatgcatcg cagcgagcgc ctcgcggaag
     1321 ctcggcgcgc cgaggcggcg caaggtcgcc gcgtcgatcc attccggcat tgccggcagg
     1381 cgctgcatcg cgccttgcgc ggcgcgggcg acgacgcgcg gatagagtcc ctcggtcagg
     1441 ccatagaccg gctcgaccgg cggcaatttg gcgagggctt ccgcgtccat gacgcggtcg
     1501 ggatggacca tctgcaaatg gccgtcccag atttcgagct tgcccgacac ccagcgcgtc
     1561 gcgccgaccg gcagcgattt ttttatccaa tcggtattgg cgaggaaaaa taccaattcg
     1621 acatcgccgg tctcgtcttc gaccaggacc ttaaacggcg ctttcgagcg tggactcggc
     1681 ggcggcctgt gctcggcgac cttgacctcg atcgtggcga tttgccccca gaccgcgtcg
     1741 cggattttcg gccggctgcg ccggtcgagc ccggcatagg gcaggtgaaa cagcacgtcg
     1801 acaaggagcg cgccgcggcc gtcgcggccg agcagccgat cgaacagctt ggcgtttttc
     1861 ggtccgattc cgggcagggt cgcgaccggc gcgaagagcg ggtccagaag ggcggggcgc
     1921 atgactttgt tatagccgag gacctgccgg gcaagaacgc gatggaactc cctcgcccgc
     1981 cgcagcttat ttcccggcgg cgggcctggc gagaagcctt ctcgccgcgg cgatggagat
     2041 ggtcatggct catgcaagca agacccatag cggcgccggc gcgaagggca aaagcgccgg
     2101 gaccggcgcc atgaccgatt tgccgaagga taagatcgaa gaaaacatgg ccctgtccaa
     2161 tcgcgacaag gcgcgccatc cgaaggggcg cgggcttgac agcaaggacg tgcagaccaa
     2221 gcagcttcag gaccatgccg gcaaccggct cgatgacgaa tgagggcgcc ggcgcgacaa
     2281 agtacgttcg cctgcgctag gcccggctga ccctgccgat cgcctcttcg cggatgaagt
     2341 catagaccga ctgcgccgcc ggcgacaggg cgcggttgcg caccgcgatc aggctatagg
     2401 gctgcaccac gatcagccgc tgggtcggaa gcacctcgac cggtccgacc gcgctttgcg
     2461 ccatgaattt cgccgcctcg accgagatcg gcgcgatcgc gttcgatttg gccaccatga
     2521 tcatggtcag gagcagcgac gtcgtgttcg acaggcgctc gggcatggcc atgttctcgg
     2581 ccaggaacat cgtctcgatc gtctttctca gcggcgtgcc gcgcggctgg acgacccatt
     2641 cgtaggcgcc gaggtctttg accgagacag gcccgcgccc ggtcagcgga tggccgcgcc
     2701 gcacgatcag gcaggcctcc tcgacgccga tcgtgtagca gtcgaaaagc cgcggattga
     2761 gatcgtccgg gacccgcgcg atgatgaaat cgagacgcgc ggcgagaaga tcgcgggcca
     2821 gcacgttgct cgcgtcgatc ttgatcgtca gctcgatctt gggatggatg gcgcgcagct
     2881 tggcgatggc cggcgcggcg aggtcgatcg ccggcccggt caccgcgccc aaagccgccg
     2941 agccggcgcg gccgtcgcgc agatcggaaa tttcccgctc cgcctgcgcc aattgcagca
     3001 gcaccgaacg cgcatgccgc gccagcgatt ggccgagcgg ggtcaggcga accccgcggg
     3061 cgaggcgctc gcacagcggc gcgccgagca gggcctccat ctcggcgatc atgcgcgagg
     3121 cggcgggctg ggaaatattg atcgcctgcg cggcggcgct gaccatttcg tgctcttcga
     3181 gcgcaacgat catccgcaaa tgcgacagtt tgaactggac gccgggcgag gcggcgcttc
     3241 ggccggccgc cgcctcgtcg ggatcgcggc cgagcctgtc ggtgaaatcg tccatggccg
     3301 cgcccccgga aactcggccg atctatacca attacggata gatggcgatt gtcatattat
     3361 ttgactgtca taggaaattc caccagcatg cccaaaagcg agaacgtggc gctgctcaat
     3421 cgccatcgaa gggacgggtt cgaggagaag gattgcggat gtccgctgca acggccgaaa
     3481 gcgcgtcctt cgccaatatc gttcgcctcg ccggcgtgca gaaatatttc ggcgccgtgc
     3541 atgcgctgag aaacatcaat ctggccgtcg gccgcaacga gatcgtcggg ctaatcggcg
     3601 ataacggcgc cggcaagtcg accctgatca agacgatcac cggcgtgcac aagccgacat
     3661 cgggccgcat cttcgtccgc gacaaggaga tcgatctcga cgcctattcg gtcggcaagg
     3721 cgcatgattt gtcgatcgag accgtgtacc aggataagtc gctcggcgag aagcagccgc
     3781 tttggcgtaa tttcttcgtc ggacgccaga tcaccaaccg cttcggcttc atcgacgtga
     3841 agagggagaa ggaggtcgcc aacgacatcc tcatcaacac gatcggtttt cgcggcgtcg
     3901 gcatttcggt ggactcgacc gttcagcttc tatccggcgg cgagcggcaa ggcatctcga
     3961 tcggccgcgc catgcacttc aattcggacc tgatcatcct cgacgagccg accgcggcgc
     4021 tcggcgtcaa ggaagtcagg aaagttctgg aattcgtgcg ccgcatcaag gagtcgggcc
     4081 gcgcctcgat ctatatcgag cacaatctcg cccatgtcca cgaagtcgcc gatcgcctcg
     4141 tcgtgctcga
//
LOCUS       DATGJW010000410         5791 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1217342, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000410 DATGJW010000000
VERSION     DATGJW010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5791)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5791)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5791
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1217342"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VKU03_12340"
     CDS             <1..567
                     /locus_tag="VKU03_12340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011473725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HLJ72101.1"
                     /translation="KSARDLERAVNAELPPLKLERAEFSVAIATDPAAIAAEGCDRIE
                     FHVRTNPGSRAGPLMKVASGGELARFMLALKVALADRGSAPTLVFDEIDTGVGGAVAD
                     AIGQRLARLAVRAQVLAVTHAPQVAARSTSHFQIAKSAVGKGERVATRVQALDGPARR
                     EEIARMLAGATITDEARAAARRLIESAG"
     gene            623..1273
                     /gene="bluB"
                     /locus_tag="VKU03_12345"
     CDS             623..1273
                     /gene="bluB"
                     /locus_tag="VKU03_12345"
                     /EC_number="1.13.11.79"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013418774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0032363 - FMN catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5,6-dimethylbenzimidazole synthase"
                     /protein_id="HLJ72102.1"
                     /translation="MSESAPPEFDDAFREKFETLIRWRRDVRRFKPDLITPDLISKLL
                     SFAALAPSVGLSQPWRFVTVDDPMRRAAIRRNFEAANAEALAACVGERQSLYAKLKLA
                     GLDQAPCHLALFVEPSPEQGHGLGRLTMPETVAYSAVMAAHTLWLAARAHRIGLGWIS
                     ILEPSAVAKTLEAPSHWRFIGYFCLGYPEAEDDVPDLERRGWERRRENMRAVVLRR"
     gene            1461..1634
                     /locus_tag="VKU03_12350"
     CDS             1461..1634
                     /locus_tag="VKU03_12350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339753.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72103.1"
                     /translation="MASEKRRDSEAEAKRKRLAAALRENLKRRKAQQRGRAGQDVQKT
                     LEAESDLGDNHKF"
     gene            1672..2961
                     /gene="murA"
                     /locus_tag="VKU03_12355"
     CDS             1672..2961
                     /gene="murA"
                     /locus_tag="VKU03_12355"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019195289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLJ72104.1"
                     /translation="MDRIQIRGGSRLNGEIRISGAKNAALPLMIASLLTDETLTLENL
                     PRLADVSLLVRILGNHGVDYSLNGRRAGENADYGQTAHLTAREIVDTTAPYDLVSKMR
                     ASFWVIAPLLARAGEARVSLPGGCAIGTRPVDLLLMALESLGAKIEIDAGYVVATTPK
                     GFVGGEVNFPKVTVGGTHTAIMAAATANGASIVRNAAREPEVKDLADCLNKMGARIHG
                     AGTSTIEITGVAKLSGARHSVLPDRIETGTYATAVAMTGGDVLLKGAVADHLQESLDA
                     LSAAGASVSSDNQGVRVKRNGAGISAVDIETAPFPGFATDLQAQFMALMTKAKGVSRI
                     RETIFENRFMHVQELARLGAHIKLEGDTAIVEGVAKLRGAPVMATDLRASVSLVIAAL
                     AAEGETMVNRVYHLDRGFENLEHKLGACGAEIQRISG"
     gene            2965..3438
                     /locus_tag="VKU03_12360"
     CDS             2965..3438
                     /locus_tag="VKU03_12360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HLJ72105.1"
                     /translation="MQDAPTDLKLIALDGEDLAVISAHVQDALVRRSEMTYLAGQKRF
                     ALGAARYDWAADAAGRKERVGAALRFDRVLKVTQIGLSGLRPEAILNLLAVSLDERDP
                     PAGVVTLTFSGGAAVRLEVECVEGELRDIGPREQVKVCPGHALIDDSKVGSSVTR"
     gene            3572..4873
                     /gene="hisD"
                     /locus_tag="VKU03_12365"
     CDS             3572..4873
                     /gene="hisD"
                     /locus_tag="VKU03_12365"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HLJ72106.1"
                     /translation="MPFRLDMRTADFEPQFEALLASKREVSEDVDTTVAAIIEDVRAR
                     GDEALAEYSLRFDRIDLTRLGVRVGDNEIGAAVAACERETLAALELAHNRVLAYHQRQ
                     KPQDVSFVDPLGVELGWRWRPIEAVGLYVPGGAASYPSSVVMNAVPAKVAGASRIVMA
                     VPAPEGHINPLVLAAARLAGVDEVYRVGGAQAIAALAYGTGSIKPVSKIVGPGNAYVA
                     AAKRRVFGVVGIDMIAGPSEVIVLADSSADPDFIAADLLAQAEHDAAAQSILITDDPA
                     LAEAVVAAVAKQLHDLPRAATAGASWRDYGAILLAPSLAEALPLVDRLAPEHLEIMAH
                     DADALSQQVNNAGAIFLGGSTPEAIGDYVGGSNHVLPTARSARFSSGLGVLDFMKRTS
                     LLKCDAKALEALGPAAIALGRAEGLDAHARSVAMRLERRSP"
     gene            4870..5367
                     /locus_tag="VKU03_12370"
     CDS             4870..5367
                     /locus_tag="VKU03_12370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HLJ72107.1"
                     /translation="MSGSEAAAKARRRLVEVTLDETSIGRGSPDQEHERAIAIYDLVQ
                     ENSFALPDRDDGPYKLRVALQDGKLALDIEPSRGDERAEHVLPLASFRTLLKDYFLIC
                     ESYYAAVRTATPTQIEALDRNRRAIHDEAAAIVSERLAGKIEIDSETARRIFTLISAL
                     HWKKT"
     gene            5364..>5791
                     /locus_tag="VKU03_12375"
     CDS             5364..>5791
                     /locus_tag="VKU03_12375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334761.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L11 methyltransferase"
                     /protein_id="HLJ72108.1"
                     /translation="MSAAIIDRRAFILANTRVLPVPHAPEIALHLADEAVALWQKTEE
                     ELGAIGLPPPFWAFAWAGGQALARYVLDHPEVVVGKRVLDFASGSGLVAIAAAKAGAK
                     NVEACEIDEFAIDAIGLNAALNEVAVAPRLGDLVGSDEDW"
BASE COUNT          991 a         1847 c         1889 g         1064 t
ORIGIN      
        1 aagagcgcgc gcgacttgga gcgtgcagtc aatgccgaac tgccgcctct gaagctcgaa
       61 cgcgccgaat tttctgtcgc catcgcgacc gatccggcgg cgatcgccgc ggaaggttgc
      121 gatcgcatcg aattccacgt ccgcaccaac cccggctcgc gcgccggccc tttgatgaaa
      181 gtcgcctcgg gcggagaact cgcgcgtttc atgctggctt tgaaagtcgc gctcgctgac
      241 cgtggctcgg cgccgacttt ggttttcgac gagatcgaca ccggcgtcgg cggcgcggtc
      301 gccgacgcga tcggccagcg cctggcgcgt ctcgccgtgc gggcgcaggt gctggccgtt
      361 actcatgccc cacaagtcgc cgcgcgttcg acgagccatt tccaaatcgc caagagcgcg
      421 gtcggtaagg gcgagcgggt ggccacgcgg gtgcaggcgt tggacggtcc cgcccgccgc
      481 gaagagatcg cccgcatgct ggccggcgcg acgatcacgg acgaagcgcg ggcggcggcg
      541 cgacggctga tcgagagcgc gggatagggc cacaagattt tcagaggcgt attgtcaaaa
      601 aaattcccgc tatactccgt ccatgagcga aagcgccccg cctgagtttg acgacgcctt
      661 tcgcgaaaaa ttcgagacat tgatccgctg gcggcgcgac gtcaggcgct tcaagcccga
      721 tctcatcact cccgatctca tctcaaagtt gctcagcttc gcggctttgg cgccttcggt
      781 cggccttagc cagccttggc gttttgtgac tgtcgacgat ccaatgcggc gcgcggcgat
      841 acgccgaaat ttcgaggccg caaacgccga ggcgttggcg gcctgtgtcg gcgagcgcca
      901 atccctttac gccaaactga agctcgccgg tctcgatcag gcgccctgcc atctcgccct
      961 tttcgtcgag ccttcgcccg agcaaggcca cggtctcggc cgcctcacca tgccggagac
     1021 ggtcgcctat tcggctgtca tggcggcgca tacgctttgg cttgccgcgc gcgcccatcg
     1081 aatcggcctc ggctggatct cgatcctgga gccttccgcg gtggctaaga cgctcgaggc
     1141 tccgtcacat tggcgcttca tcggctattt ttgccttggc tatccggaag cggaggatga
     1201 cgtccctgac ctcgagcgtc gcggctggga gcgtcgcagg gagaatatga gggccgtcgt
     1261 cttgcgacgt taactctggc ctaaagctcg cgcttcaaaa ttctggcggt atgtaccgcc
     1321 atcatcacgt tgttagagcg gttgccgcgg gtcgaagctg cggctcgaag ctgcgtccgc
     1381 ctccgcccgc gcccttgccc tccctttacc atcggcctat ctagccgccg acgcggcaaa
     1441 ttcgccgcaa gggagccgca atggcgtcgg aaaagaggcg cgacagcgag gctgaggcca
     1501 agcgcaagcg tctggcggcc gctttgcgtg aaaatctcaa gcgtcgcaag gcgcaacagc
     1561 ggggccgggc cggtcaagac gtgcaaaaga cgctagaagc cgagtccgac ctcggcgata
     1621 atcataaatt ctagcgaatc gcgcggagcc acgcgcgttt aaggggtcac aatggatcgg
     1681 attcagatca gaggcgggtc gcggctaaac ggcgagatcc ggatttccgg cgccaagaat
     1741 gcggctttgc cgttgatgat cgcgagtctc ctgacggatg agaccctgac cctcgagaac
     1801 ctgccccgcc tcgccgatgt cagccttctg gtgcgcattc tcggcaatca cggcgtcgat
     1861 tattcgctca acggcagacg ggccggcgaa aacgccgatt acggccagac cgcgcatctg
     1921 accgcgcgcg agatcgtcga cacgaccgcg ccctatgatc tggtttcgaa gatgcgggca
     1981 agcttctggg tcatcgcccc gctcctcgcc cgcgctggcg aagcccgcgt ttcgctgccg
     2041 ggcggctgcg cgatcggcac caggccggtc gaccttctcc taatggcgct ggagagtctc
     2101 ggcgcgaaaa tcgaaatcga cgccggctat gttgtggcga cgacgccgaa gggcttcgtc
     2161 ggcggcgaag tcaatttccc gaaagtgacg gtcgggggca cccataccgc gatcatggcg
     2221 gccgccacgg ccaatggcgc gagcatcgtc cgcaacgccg cacgcgagcc cgaggtcaag
     2281 gatctcgccg attgcctcaa caagatgggc gcacgcatcc atggggccgg tacgtcgacg
     2341 atcgagatca ccggcgtcgc aaagctttcc ggcgcgcgcc attcggtcct gcccgaccgt
     2401 atcgagaccg gcacttacgc cacagccgtc gcgatgaccg gcggcgatgt tctgctcaaa
     2461 ggcgcggtcg ccgatcatct gcaggaatcg cttgacgcct tgagcgcggc gggcgcaagc
     2521 gtttcgagcg acaatcaggg ggtcagggtc aagcgcaacg gcgcgggtat tagcgcggtc
     2581 gatatcgaaa cggcgccctt tcctggcttc gccaccgacc tgcaagccca gttcatggct
     2641 ttgatgacca aggccaaggg cgtctcgcgc atccgggaga cgattttcga aaaccgcttc
     2701 atgcatgttc aggagctggc gcggcttggc gcccatatca agcttgaagg cgatacggcg
     2761 atcgtcgaag gcgtcgccaa attgcgcggc gcgccagtga tggccaccga tctgcgcgcc
     2821 tctgtctcgc tggtcatcgc ggcgctcgcc gctgagggcg agaccatggt caatcgcgtt
     2881 tatcacctcg atcgcggctt cgagaatctc gagcacaagc ttggcgcttg cggggctgag
     2941 attcagcgca tatcgggttg agcgatgcag gatgcgccga ccgacctgaa attgattgcg
     3001 ctcgatggcg aagacctcgc cgtgatctcg gcgcatgttc aggacgcttt ggtgcggcgc
     3061 agcgagatga cctatctcgc gggacagaag cgtttcgcgc tcggcgctgc acgctatgat
     3121 tgggcggccg acgccgcggg ccgcaaagag cgcgtgggcg cggcgctgcg gttcgatcgc
     3181 gtcttgaaag tcacgcagat cggcttgtcc ggcctgaggc ctgaagcgat cctcaatctg
     3241 ctcgccgtca gtctcgacga gcgcgacccg ccggccggcg tcgtgaccct gactttttcg
     3301 ggcggcgcgg cggtgcggct cgaagtcgaa tgcgtggaag gcgaactgcg cgacattgga
     3361 ccgcgcgaac aagtcaaagt ctgccccggt catgcgctga tcgacgattc gaaggttggc
     3421 tcaagcgtga cgcgatgaga tccgtctaca agggtcgctt tcgagcgcca ggggatgcga
     3481 acgagcgcgc gatgtcctca ttccgagagt tcggcgcgcc cctccttcct aatcctcctc
     3541 cgcaaggggg gagggaatcg cgagatttct gatgcctttt cgcctcgaca tgaggacggc
     3601 cgatttcgag ccgcaattcg aagcgctgct cgcctccaag cgtgaagtct ccgaggacgt
     3661 cgatacgacg gtggctgcga tcatcgagga cgtgcgcgcc cgcggcgatg aagcgctcgc
     3721 tgaatattcg ctgcgtttcg atcggatcga tctgacgcgg ctcggcgtcc gggttggcga
     3781 caatgaaatc ggcgcggcgg tcgccgcgtg cgagcgcgag actttggcgg cgctcgaact
     3841 ggcccataac cgggtcctgg cgtaccatca gcgccagaag ccgcaggacg tctctttcgt
     3901 cgacccgctc ggcgtcgagc tcggctggcg ctggcgcccc atcgaagcgg ttgggcttta
     3961 tgtgccgggc ggcgcagcga gctatccctc ttcagtggtg atgaatgcgg ttccggccaa
     4021 agtcgcgggc gcctcgcgca tcgtcatggc ggtgccagcg ccggagggcc atatcaaccc
     4081 gctggtgctg gctgcggcgc ggcttgcggg cgtggatgaa gtctatcgcg tcggcggcgc
     4141 ccaggcgatc gccgcgcttg cctatggcac aggttcgatc aagccggtca gcaagatcgt
     4201 cggcccaggc aatgcctatg ttgcggcggc caagcgccgc gtcttcggcg tcgtcggcat
     4261 cgatatgatc gccggacctt cggaagtgat cgtactggcc gattcttccg ccgatcccga
     4321 cttcatcgcc gccgatcttc tcgctcaggc ggaacatgac gcagcggcgc aatcgatcct
     4381 gatcaccgat gatccggcgc tcgccgaagc ggtcgtcgcc gccgtcgcaa agcaactcca
     4441 cgatctgccg cgggcggcta ccgccggcgc gagttggcgc gattacggcg cgatccttct
     4501 tgcgccgtct ctcgccgagg cgctgccgct cgtcgatcgc ctggcgccgg agcatctcga
     4561 aatcatggcg cacgacgccg acgcgttgag ccagcaggtc aacaacgccg gcgcgatttt
     4621 tcttggcgga tcgacgccgg aggcgatcgg cgattatgtc ggcggctcca accacgttct
     4681 gccgaccgcc cggtcggccc gtttttcctc gggcctcggc gtgttggatt ttatgaaacg
     4741 cacctcgctg ctgaaatgcg acgcgaaagc gctggaggcg ctgggcccgg ccgccatcgc
     4801 gctcggccgc gcggaggggc tcgacgcgca cgcgcgctcg gtagcgatgc gtctcgagcg
     4861 gcgttcgcca tgagtggttc ggaggcggcc gccaaggcgc ggcgtcgctt agtcgaggtg
     4921 acgctcgacg aaacctcgat cggccgcggc tcgcctgacc aggagcatga gcgggcgatc
     4981 gcgatttatg atctcgttca agaaaatagt ttcgcgctac cggatcgcga cgacgggcct
     5041 tacaagcttc gcgtcgcgtt gcaggacggc aaactggcgc tcgacattga gccgtcaagg
     5101 ggcgacgaga gggctgagca tgtcctgccg ctcgcgtctt ttcgcacttt gctcaaggac
     5161 tactttctga tctgcgagag ctattacgcg gcggtgcgca cggcgacgcc gacccagatc
     5221 gaggcgcttg accgcaaccg gcgcgccatc catgacgaag cggcggcgat cgtctccgaa
     5281 cggctcgccg gcaagatcga gatcgattcc gaaacggcgc gccggatctt cacattgatc
     5341 tcggcgcttc attggaagaa gacttgagcg cggcgatcat cgatcgtcgc gccttcatcc
     5401 tcgccaatac acgcgttctt cccgttccgc atgcgcccga gattgcgctg catcttgccg
     5461 acgaagcggt cgcgctctgg cagaagacgg aggaagaatt gggcgcgatc ggcctgccgc
     5521 cgcctttctg ggccttcgcc tgggcgggcg gacaggcgct ggcgcggtac gttctcgatc
     5581 atcccgaggt tgtcgtgggt aaaagagtgc tcgacttcgc gtccggctcc ggcctcgtcg
     5641 ccatcgccgc cgccaaggcc ggcgccaaaa acgtcgaagc ctgcgagatc gatgaatttg
     5701 ccatcgacgc gatcgggctc aatgcggcgc tgaacgaagt cgccgtcgcg ccgcggctcg
     5761 gcgatcttgt tggctccgac gaggattggg a
//
LOCUS       DATGJW010000411         3057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_910618, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000411 DATGJW010000000
VERSION     DATGJW010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3057
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_910618"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1415
                     /locus_tag="VKU03_12380"
     CDS             <1..1415
                     /locus_tag="VKU03_12380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="sigma-54 dependent transcriptional regulator"
                     /protein_id="HLJ72109.1"
                     /translation="AESGEGALARLQTPDRPPIDLAILDLVMPDLDGMGVLERMRKAE
                     ITVPAIVQTAHGSIETVISAMRAGALDFVVKPVGAERLQVSIKNALRVDALEDELRRA
                     TRRSAGELTFRDIITKAENMGRVIRLAERAAKSNIPVLIEGESGVGKELMARAIQGAS
                     ERRGKPFVAVNCGALPENLVESILFGHEKGAFTGATEKHIGKFAEANGGTLFLDEIGE
                     LPLETQVKLLRALQEGEIDPIGARRPVKIDIRLVSATNQNLVDLVRRGRFREDLFYRL
                     NVFPITIPPLRARTQDVPDLARRFLARFCAEEGKRIRGLSSEALALLSAYPWPGNVRQ
                     LENAVFRAVVLADADELTAAEFPQIAAQVAGFDVRIPPIQTPAPPSERAEPPPSEHSA
                     PFEPRDPNVMRLLDESGDVRKLEEIEAEAIKFALAHYRGQMSKMARKLGIGRSTLYRK
                     MKEIGVETGEPGDDADDAAA"
     gene            1510..>3057
                     /locus_tag="VKU03_12385"
     CDS             1510..>3057
                     /locus_tag="VKU03_12385"
                     /inference="COORDINATES: protein motif:HMM:NF040455.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLJ72110.1"
                     /translation="MKKGGWPAGLASLCALALASHAGFIAGARAEDRNAARAASALIE
                     PELPPDAGGPPAIAGSSEAAPKPPLRQGGESADEPSPAQLAAPEQADEATKSDPEAVF
                     AGETLEGARPRDIAAALATLPAAEGKTSPVAGLPNWAAARDALERFYLARGDMPIFTD
                     AKGFSAAGRAAIGRLDRADEDGLDLSGFAIPDANAAIDNPADLAAAEIGLAEAVIAYA
                     SQASGGRINPARISPLITARPAVADPEAALETVAAAEDKDAALAAFNPPQKGYRALRE
                     RLAALRAAYPAAAANLYRRAPKPEGNGRGAALIRPNFEIGGRQAKAERRARGAQIAAA
                     DAPQPDTLADDRLDPAMAQAIARRGPRAIEAAILANMEMWRWQPRDMGEERIEINVPD
                     FRLRLVKDDETLHSARIIVGKPETQTPIFSNAVRYIEINPAWRVPPSIVRKEMLPKLA
                     DDPDYLTRMGYEVTKHGDTISVKQPPGEKNALGRIAFMFPNEHSVYLHDTPARSLFGS
                     SRRAFSHG"
BASE COUNT          513 a         1088 c         1017 g          439 t
ORIGIN      
        1 ccgccgaatc gggcgaaggc gcgctggcgc ggctgcaaac gcccgaccgt ccgccgatcg
       61 acctcgcgat cctcgatctt gtcatgcccg atctcgacgg gatgggcgtg ctcgaacgca
      121 tgcgaaaggc cgagatcacc gtcccggcca tcgttcagac cgcgcatggc tcgatcgaga
      181 cggtgatttc ggcgatgcgc gccggcgcgc tcgatttcgt cgtcaagccg gtcggcgccg
      241 agcgcttgca agtctcgatc aagaacgcct tgcgcgtcga cgcgctcgag gacgaattgc
      301 gccgcgcgac ccggcgcagc gccggcgaat tgacgttccg cgacatcatc accaaggccg
      361 agaatatggg ccgggtgatc cggctcgccg agcgcgccgc caagtcgaac attcccgtcc
      421 tcatcgaggg cgaatccggc gtcggcaagg aattgatggc gcgcgccatc cagggcgcct
      481 cggagcggcg cggcaagccc ttcgtcgccg tcaattgcgg cgccctgccg gagaatctcg
      541 tcgaatccat cctgttcggc cacgaaaaag gcgccttcac cggcgcgacc gaaaagcata
      601 tcggcaaatt cgccgaggcc aatggcggca cgctgtttct cgacgaaatc ggcgaattgc
      661 cgctggagac gcaggtcaag cttttgcgcg ccctgcagga aggcgagatc gacccgatcg
      721 gcgcccgccg tccggtcaag atcgacatcc ggctggtctc ggcgaccaat cagaatctcg
      781 tcgacctcgt ccgccgtggc cgcttccgcg aggacctttt ctatcgcctc aacgtctttc
      841 cgatcacgat tccgcccttg cgggcgcgga cgcaggacgt gcccgacctg gcgcggcgct
      901 ttctcgcccg cttctgcgcg gaggaaggaa aacgcatccg cggcctttcc agcgaggcct
      961 tggccctgct ctcggcctat ccctggccgg gcaatgtgcg ccagctcgaa aacgcggtgt
     1021 tccgcgccgt ggtccttgcc gacgccgacg aattgaccgc cgccgaattt ccgcaaatcg
     1081 ccgcccaggt cgccggcttc gacgtgcgca ttccgccgat tcagacgccg gcgccgccga
     1141 gcgagcgcgc cgagccgccg ccgagcgagc attctgcgcc gttcgagccg cgcgatccca
     1201 atgtgatgcg gctgctcgac gaatcgggcg atgtgcgcaa gctcgaagag atcgaggcgg
     1261 aggcgatcaa gttcgccctc gcccattatc gcggccagat gtcgaaaatg gcccgtaagc
     1321 tcggcattgg gcgctcgacc ctttatcgca agatgaagga gatcggcgtc gaaaccggcg
     1381 agccgggcga cgacgcggac gatgccgccg cctgatcaag actgcgttcc cgagccatgg
     1441 ccggccggcg cgaaaggcgc tagtttggcg ctaggcgctc ggggcgttaa gcgctgaggt
     1501 tgtggggtga tgaaaaaagg gggctggccg gcgggcctgg cgtcgctttg cgcattggcc
     1561 ctggcgagcc atgccggctt cattgccggc gcgcgggccg aggatcgaaa cgccgcgcga
     1621 gccgcctccg ctttgatcga gcccgaattg ccgcccgacg ccggcgggcc gccggcgatc
     1681 gccggatcaa gcgaggccgc gccgaagccg ccgctgcgcc agggcgggga aagcgcggac
     1741 gagccctcgc ccgcgcaact cgccgcgccg gagcaggcgg acgaggcgac gaaaagcgac
     1801 cccgaagcgg tcttcgccgg cgagacgctg gaaggcgccc gcccccgcga catcgccgcc
     1861 gcgctcgcca ccctacccgc ggccgagggc aagacaagcc cggtcgccgg cctgcccaac
     1921 tgggcggccg cccgcgacgc cctggagcgc ttctatctcg cccgtggcga catgccgatc
     1981 ttcacggacg ccaaggggtt cagcgccgcc ggccgggcag cgatcggccg gctcgaccgc
     2041 gccgacgaag acggactcga tctttcgggc ttcgccattc ccgacgccaa tgcggcgatc
     2101 gacaatccgg ccgacctcgc cgccgcggag atcggccttg ccgaggcggt catcgcctat
     2161 gcgtcccagg cgagcggcgg gcggatcaac cccgcccgca tttcgcccct gatcacggcc
     2221 aggccggcgg tcgccgatcc cgaggccgcg ctcgaaaccg tcgccgcggc cgaggacaag
     2281 gacgcggcgc tcgccgcctt caacccgccg cagaagggct atcgcgcgct gcgcgagcgg
     2341 cttgcggcgc tgcgcgccgc ctatcccgcc gccgcggcga atttgtaccg gcgcgcgccg
     2401 aagccggagg gcaatgggcg cggcgcggcc ttgatccgcc cgaacttcga aatcggcggc
     2461 cgccaggcca aggcggagag gcgcgcccgc ggcgcgcaaa tcgccgccgc cgacgcgccg
     2521 cagccggata cgctggccga cgaccgtctc gacccggcca tggcgcaagc gatcgcccgg
     2581 cgcggcccgc gcgcgatcga agccgccatc ctcgccaata tggaaatgtg gcgctggcag
     2641 ccgcgcgaca tgggcgaaga gcgcatcgag atcaatgtcc ccgatttcag gctgcgcctg
     2701 gtcaaggacg acgaaacgct gcattcggcc cggatcatcg tcggcaagcc ggagacgcag
     2761 acgccgatct tctcgaacgc ggtgcgctac atcgagatca atccggcatg gcgcgtgccg
     2821 ccgtcgatcg tacgcaagga aatgctgccg aagctcgccg acgatcccga ctatctcacc
     2881 cgcatgggct atgaggtgac caagcacggc gacacgatca gcgtgaagca gccgccgggc
     2941 gagaagaacg cgcttggccg catcgccttc atgtttccga acgagcattc ggtctatctg
     3001 cacgatacgc cggcgcgcag cctgttcggc tcttcgcgcc gcgctttcag ccatggc
//
LOCUS       DATGJW010000412         4451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_701684, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000412 DATGJW010000000
VERSION     DATGJW010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4451
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_701684"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            331..1014
                     /locus_tag="VKU03_12390"
     CDS             331..1014
                     /locus_tag="VKU03_12390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909858.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ72111.1"
                     /translation="MTLDAVLSRPELRVAPLARDTLQERAYRRICDLILDGEIAPGQV
                     VTIQTLAEAFGVSAMPVREALKRLTASGALSLVTGRSIGVPRLSLDRLADLRRVRLAV
                     EGLASEWAAVLMEADDLARLDAHYDLLSRASTEENVKDYLRANRAFHFTIYNAARSPT
                     LVAMIETLWLQIGPYLNLLRGSGNYAASNATHRAIVEALTARDGKSAAKALRADINDA
                     YEILAKMLA"
     gene            complement(1025..1495)
                     /locus_tag="VKU03_12395"
     CDS             complement(1025..1495)
                     /locus_tag="VKU03_12395"
                     /inference="COORDINATES: protein motif:HMM:NF012601.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dCMP deaminase"
                     /protein_id="HLJ72112.1"
                     /translation="MNVADIVPRGANMQKAASVAALSPNRIRQVGAVLATPGGVEIAA
                     CNDFPPGIRDLDERHLGDGRFVWMEHAERRAIFEAARRGVATNGAHMTTTFFPCIDCA
                     RAIVEAGFRCLDAPPPAFEDPVWGASFERSQVILQEGGVEIRIVGTADAPPPRA"
     gene            1674..2132
                     /locus_tag="VKU03_12400"
     CDS             1674..2132
                     /locus_tag="VKU03_12400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012061563.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RbsD/FucU family protein"
                     /protein_id="HLJ72113.1"
                     /translation="MLKLIDPSLNADVLHALKSMGHGDELVLCDTNFPADSVARHTVL
                     GRLLHMDNVSAARAARAILSLFPLDSFVEKPALRMEIAGKPDEIPPVQSEVQSEIDRA
                     EQKAWPMGSIERMAFYDRARKAYCVIRSGERRFYGCFIFKKGVVSPDVVF"
     gene            2144..3085
                     /locus_tag="VKU03_12405"
     CDS             2144..3085
                     /locus_tag="VKU03_12405"
                     /EC_number="2.7.1.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribokinase"
                     /protein_id="HLJ72114.1"
                     /translation="MASQKRGVAILGVFVADLAFRAGRMPGIGETIIGSGFKIGPGGK
                     GSNQAVAAARLGVPVTFISRLGKDEFGALALAAWRREGIEARVIESADEGTGAAFIYV
                     NEQSGENAIIVAPGAAASIGEADVEAAADAIGQAAVFVTQLEQPVAAARRGLELAKAA
                     GAVTIFNPAPAVALDDAIYPFCDFVTPNENEAALLAGLRVSTVAEARKAGDVFLKKGA
                     RTALITLGETGALFHDGDRSTLIPAFRAGPVVETTGAGDAFTGGFAAALADGAEPIEA
                     ARFGCAVAGISVTRAGTAPSMPKRGEVEDLLARAKRS"
     gene            3087..3845
                     /locus_tag="VKU03_12410"
     CDS             3087..3845
                     /locus_tag="VKU03_12410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008875430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HLJ72115.1"
                     /translation="MRYELMLPREIRAAIERRWPVVLPLGVLEYHGEHMAVGMDTLAV
                     MKILEILEKEIDLVILPPFYYGAASYAVEPPEGMGTLHVDAEKLLPLAEDMFRGLLRI
                     GFRNIHILVHHQTENFVAGMPTDLAFKFAARQAIFAFLETERGEGWWGGEKMADYYAA
                     QAAGDDPFNWIKAHPLMTPDSMEGYPFDHAGQGETSLLMALCPEGVDLDRLTDDKWYV
                     RSARRSSVSLGEKGRDLILARLRKILGAPARGPA"
     gene            4045..>4451
                     /locus_tag="VKU03_12415"
     CDS             4045..>4451
                     /locus_tag="VKU03_12415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008524000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HLJ72116.1"
                     /translation="MRVGVYNQLMAMQASRRQVLKGAASATAALAASGPLGGLTSSAF
                     AADSVVAQIMKIPGAGNGSPTDADWQKVGELCLGPTKANVQPGEFKGVEFAFMGLNNQ
                     NLHNFLFRGFLTPWEAYTGAKIKWIDLAQADYNA"
BASE COUNT          767 a         1454 c         1480 g          750 t
ORIGIN      
        1 gagactctta tggctggcca tggccttgac catattggcg gcctcggcct gccagtcatt
       61 gccaatatag ctcatgctga ggaaaatctt atgcttttgc gcggcgtccg ccgcgtgcgg
      121 cgcgaccagg cctaaagcca gcgcgcccgc caaaagctgc cgagctccac ccgtcatggt
      181 cttcctcccg gtttgtcgcc tctttttgcc ggcgatctcg tgatcataga tcatagatca
      241 atcgatgcaa gccggtgcga tttgcgctac tgcttgcgtc ggcggccgat tccggagact
      301 ggaacaggaa ttgggcgggg acgggcgcgt atgacgttgg acgcggtgct gagcaggccg
      361 gagctgagag tggcgccgct ggcgcgcgac acgctgcagg agcgcgccta tcgccggatt
      421 tgcgacctga tcctcgacgg cgaaatcgcg ccgggccagg tggtgacgat ccaaacgctc
      481 gccgaggcgt tcggcgtcag cgccatgccg gtgcgcgagg cgctgaagcg cctgaccgcg
      541 tcgggggcgc tcagccttgt gaccggccgc tcgatcggcg tgccgcgcct cagcctcgat
      601 aggctcgccg atctgcgccg cgtgcgcctc gcggtcgagg ggctcgcctc cgaatgggcc
      661 gccgtgctca tggaggcgga cgatctcgcc cgcctcgacg cgcattacga tctgctcagc
      721 cgcgcctcga cggaggagaa cgtcaaggac tatctgcgcg ccaaccgcgc cttccatttc
      781 acgatctaca acgccgcgcg ctcgccgacc ctcgtcgcga tgatcgagac gctttggctg
      841 cagatcgggc cctatctcaa cctgctgcgc ggctcgggca attacgcggc ctccaacgcg
      901 acacatcgcg cgatcgtcga ggcgctgacg gcgcgggacg gcaagtccgc cgccaaggcg
      961 ctgcgcgccg atatcaacga cgcttacgag attctcgcga aaatgctggc ttgagcgccc
     1021 acgttcaagc gcgcggaggc ggcgcgtcag ccgtaccgac gatgcggatc tcgacgccgc
     1081 cttcctggag gatgacttgc gagcgctcga acgatgcgcc ccagaccgga tcctcgaagg
     1141 ccggcggcgg cgcgtcgagg cagcgaaagc cggcttcgac aatcgcgcgg gcgcaatcga
     1201 tacagggaaa gaaggtcgtg gtcatatgcg cgccatttgt cgcgaccccg cgccgcgctg
     1261 cttcgaaaat cgcacggcgc tcggcatgct ccatccagac gaagcgcccg tcgccaagat
     1321 ggcgctcgtc gagatcgcgg atccctggcg gaaagtcgtt gcaggcggcg atctccacgc
     1381 cgcccggcgt cgccagcacc gccccaacct ggcgaatccg gttgggactc aaggcggcga
     1441 cgctggcggc cttttgcata ttggcgccgc gcgggacgat gtcggcgacg ttcacgaaag
     1501 cgctcccgat tcgctgatct tagtgtctcc gcggaacgcc ggcccgtcaa acgccccaag
     1561 tcaaacgcgc ggcctcgctt gacggcggat tgggcggacg cgatgctgcc tccttgcgcg
     1621 atgtgaaacg cgcgcgatca atctcgcgcc gacacgccga gggagggaac aagatgctga
     1681 agctcatcga tccgagcctc aacgcagacg tgctgcatgc gttgaaaagc atgggccatg
     1741 gcgacgagct tgtcctttgc gacacgaatt ttccagccga ttcggtcgcg cgccacaccg
     1801 tgctcggccg tctcctgcat atggacaatg tctcggcggc gcgcgcggcg agagccattc
     1861 tctccctgtt tccgctcgac agtttcgtcg agaagccggc gctgcgcatg gagatcgccg
     1921 gcaagccgga cgagattccg ccggtgcaga gcgaagtgca aagcgagatc gaccgcgccg
     1981 agcaaaaggc ttggccgatg ggctcgatcg agcgcatggc cttctatgac cgcgcgcgga
     2041 aggcctattg cgtgatccgc agcggcgagc gccgcttcta tggctgtttc atcttcaaga
     2101 agggcgtggt gtcgcccgac gtcgtcttct gatcgaggcg agcatggcca gccaaaagcg
     2161 cggcgtcgcc attctcggcg tcttcgtcgc cgatctcgcc ttccgcgccg gccgcatgcc
     2221 cggaatcggc gagacgatca tcggctcggg cttcaagatc gggccgggcg gcaaaggctc
     2281 gaaccaggcc gtcgccgccg cgcggctcgg cgtaccggtc acgttcatct cgcgcctcgg
     2341 caaggacgag ttcggcgcgt tggcgctggc ggcgtggcgg cgcgagggaa tcgaagcgcg
     2401 agtgatcgaa agcgccgatg aggggacggg cgccgccttc atctatgtca acgagcagag
     2461 cggcgagaac gcgatcatcg tcgcgcccgg cgcggcggcc tcgatcggcg aagccgacgt
     2521 cgaagccgcg gccgacgcga tcggccaggc cgccgtcttc gtcacccagc tcgaacagcc
     2581 ggtcgcggcg gcgcggcgcg gcttggagct cgccaaggca gccggcgccg tcacgatttt
     2641 caatccggcg ccggcggtcg cgctcgacga cgccatctac ccgttctgcg acttcgtgac
     2701 gccgaatgaa aacgaggcgg ctctgctcgc cggattgcgg gtgagcactg tcgccgaggc
     2761 gcgcaaggcg ggcgacgtgt tcttgaagaa gggcgcgcgc acggcgttga tcacgctcgg
     2821 cgagacgggc gccttgttcc acgacggcga ccgctcgacg ctgatccccg ccttccgcgc
     2881 cgggccggtc gtcgagacga ccggcgccgg cgacgccttt accggcggat tcgccgccgc
     2941 gctcgccgac ggcgccgagc cgatcgaggc ggcgcggttc ggctgcgccg tcgccggcat
     3001 ttccgtcacc agggccggca ccgcgccgtc gatgccgaag cgcggcgagg tggaagactt
     3061 gttggcgaga gccaaacgga gctgacttgc gctacgaatt gatgctgccg cgcgagatca
     3121 gggccgcgat cgagcggcgc tggccggtcg tccttccctt gggcgttctc gaataccatg
     3181 gcgagcatat ggcggtcggc atggacacgc tggccgtgat gaaaatcctc gaaattctcg
     3241 agaaagagat cgacctcgtc atcctgccgc ccttctacta tggggcggcg agctatgcgg
     3301 tcgagccgcc ggaaggaatg ggcacgctgc atgtcgatgc ggaaaagctc ttgcccctgg
     3361 ctgaggacat gtttcgcggg ctgctgcgca tcggctttcg caacatccat atcctcgtcc
     3421 atcaccagac cgagaatttc gtggcgggca tgccgaccga tctggcgttc aaattcgcgg
     3481 cccgccaggc gatcttcgcc ttcctcgaaa cggagcgcgg cgaaggctgg tggggcggcg
     3541 agaaaatggc cgattactac gccgcgcaag cggccggcga cgatcctttc aattggatca
     3601 aggcccatcc gctgatgacg ccggattcga tggaaggcta tccgttcgac catgccggcc
     3661 agggcgagac ctcgcttctg atggcgctct gccccgaggg cgtcgatctc gaccgcctta
     3721 cggacgacaa atggtacgtg cgcagcgcgc gccgctcgtc ggttagcctc ggcgaaaagg
     3781 gccgcgatct cattctcgcc cgcctgcgga aaattctcgg cgcgccggcg cgcggcccgg
     3841 cttaacgagg cgccgcccgg tttcgggtgc ggcgagaggg cgattgactt ccccgaaggc
     3901 tgcagcatac tagcatgcgg cgctgttttc tttgagcttt tgcgaacggg acggaaagag
     3961 cgagtggcgg gctcggcgct gtcgccttcg taaattcgct ctgaaagctc gccagaacga
     4021 caatagcaag ccgggaggaa agccgtgaga gtaggcgtct acaatcaact gatggccatg
     4081 caagcgagcc gccgccaagt gctgaaaggc gcggccagcg cgaccgcggc gctcgccgcg
     4141 agcggacctt tgggcggctt gacctcgtca gccttcgccg ccgatagcgt cgtcgcccag
     4201 atcatgaaaa tccccggggc cggcaatggc tccccgaccg acgcggattg gcagaaggtc
     4261 ggcgaactgt gcctcggtcc gaccaaggcg aatgtgcagc ctggcgagtt caagggcgtc
     4321 gaattcgcct ttatgggcct caacaaccaa aacctgcaca atttcctgtt ccgcggcttc
     4381 ctgacgccgt gggaggccta tacgggcgcg aagatcaaat ggatcgacct cgcccaggcc
     4441 gactacaacg c
//
LOCUS       DATGJW010000413         2923 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_490522, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000413 DATGJW010000000
VERSION     DATGJW010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2923)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2923)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2923
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490522"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..344)
                     /locus_tag="VKU03_12420"
     CDS             complement(105..344)
                     /locus_tag="VKU03_12420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72117.1"
                     /translation="MSDAFSPVLSKAHAAIVADAAGRGLKAFRRGFARDKLGPFHSPR
                     SVGVLARAGLLKWTGKTTVHLTAVGEAHAADRFAP"
     gene            complement(341..616)
                     /locus_tag="VKU03_12425"
     CDS             complement(341..616)
                     /locus_tag="VKU03_12425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72118.1"
                     /translation="MSETPTMTPAQIAEALQVDERTFLARRPRLQRDYAFPRALPGCM
                     LRWSRAQVLAWINRSTSETEPPEPLRVVAGNDVIARLEEKYVREQRR"
     gene            complement(613..915)
                     /locus_tag="VKU03_12430"
     CDS             complement(613..915)
                     /locus_tag="VKU03_12430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72119.1"
                     /translation="MRTTTLDRGDPLYRIVMNYRVAVCAAEQELVRQCRAAKTPLTIT
                     FNVVVTELLDGLARACSLGEQAETYSQDSFEAALIDGFRIHLAKYREKITVMGARS"
     gene            complement(912..1301)
                     /locus_tag="VKU03_12435"
     CDS             complement(912..1301)
                     /locus_tag="VKU03_12435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72120.1"
                     /translation="MQSDDPGAAAAPFNAAFTDSQIRRRQRIAEAAVAEASAAFPPDA
                     MRKYFFKKVAVEQLIQRQDPALIAAALADALGAQIGQLATCFKQDPDRTALNFLQLVA
                     QAARSSLEAQKRAIAELAAQAPAGEPS"
     gene            complement(1372..1749)
                     /locus_tag="VKU03_12440"
     CDS             complement(1372..1749)
                     /locus_tag="VKU03_12440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72121.1"
                     /translation="MIQSPPHPGNIAPLIALHCQKHGPQGYLAAYLRASPATPKGHDV
                     PALLQFENAAPLRFSVVVGDGGLAGAGEKALWVAIFDALAKAPSTLIQVGGERAVLDT
                     SQFTEISDRFLQACSEMMPPAAK"
     gene            complement(1869..2378)
                     /locus_tag="VKU03_12445"
     CDS             complement(1869..2378)
                     /locus_tag="VKU03_12445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72122.1"
                     /translation="MASTTLTRFLNNPTHANALSARTIAAVERVTGIRFGAGGAPPSG
                     FGEPEADAFDASRASAELAERIEALTASKNSLTVWRLRSHAIEAIGYLPGDILIVDLN
                     EPPKRGDVVCAQLYDWSRMKAETVFRLFEPPYLLAAAFDRSQLSARLIDKEVGIKGPV
                     IFSVRPRAA"
     gene            2590..>2923
                     /locus_tag="VKU03_12450"
     CDS             2590..>2923
                     /locus_tag="VKU03_12450"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ72123.1"
                     /translation="MFLWLSIFCSRSPTSNCGRGMTLDQIDARRRAAGLSMSELCRRA
                     GINTSTYQRTRQGKGGNYRRTLERLAAALENPKALSLSQLRRGSPEKETALIRATFRG
                     WAAHFARET"
BASE COUNT          501 a          882 c         1003 g          537 t
ORIGIN      
        1 gagctcggcg gccggctggc cggcgaaggg gaatggctcg atcatgcttc gtctccggac
       61 gatgcgggaa agcgtcgcga attcggcggg cgagagcgcc cgcctcatgg cgcgaagcgg
      121 tcggcggcat gggcttcgcc gaccgcggtc aggtgaacgg tcgtcttgcc ggtccatttc
      181 agaaggccgg cgcgggcgag gacgccgacg ctgcgcggcg aatggaaggg gccgagcttg
      241 tcgcgggcga agccgcggcg gaaagcttta aggccgcggc cggcggcgtc ggcgacgatc
      301 gccgcatggg ccttcgagag gacgggcgag aaagcgtcgc tcatcgccgt tgctccctga
      361 cgtacttttc ttcgagcctt gcgatgacgt cattgccggc gacgacgcgg agcggctcgg
      421 gcggctcggt ctcgctggtc gagcggttga tccaggcgag gacctgggcg cggctccagc
      481 gcagcatgca gccgggcagc gcgcgcggga acgcatagtc gcgctgcaaa cgcggccggc
      541 gggcgaggaa ggtccgctcg tcgacctgca aagcttcggc gatctgcgcc ggggtcatgg
      601 tcggcgtctc gctcatgatc gcgcccccat gacggtgatt ttctcgcgat acttcgcgag
      661 atgtatgcga aaaccatcga tgagcgccgc ttcgaaactg tcctgcgaat acgtctcggc
      721 ttgttcgccg agcgagcagg cgcgcgccag gccgtccagt aattcggtta cgacgacgtt
      781 gaaagtgatg gtcaacggcg tcttggcggc gcggcattgc cgcaccagtt cttgctccgc
      841 ggcgcagacg gcgacgcgat agttcatgac gatgcgatag agcggatcgc cgcggtcgag
      901 cgtggtcgtc ctcatgacgg ctctccggcc ggcgcttgcg cggcgagttc ggcgatggcg
      961 cgcttctgcg cctcgagcga agagcgcgcg gcctgggcga cgagctggag gaagttgagc
     1021 gcggtccgat ccggatcctg cttgaagcag gtcgcgagct ggccgatctg cgcgccgagc
     1081 gcgtcggcga gcgcggcggc gatgagcgcg ggatcctggc gctggatcag ttgttcaacg
     1141 gccaccttct tgaagaaata tttccgcatc gcgtcgggcg ggaaggcggc cgaggcctcg
     1201 gcgaccgcgg cctcggcgat gcgctggcgg cggcggatct ggctgtcggt gaaggcggcg
     1261 ttaaaaggcg cggccgcggc gcccggatcg tccgattgca tggatggccc ccaaatcagc
     1321 ggaggtgctg atttggagaa gatgcattaa tgcagcgtca attgtcaaag gctattttgc
     1381 tgctggaggc atcatctcgc tgcatgcttg gaggaagcga tcggagattt ctgtgaattg
     1441 agaggtgtcg agaactgcac gctcgccgcc gacctggatc agggtgcttg gcgcttttgc
     1501 gagcgcatca aagatcgcca cccaaagagc tttttccccg gctccggcta gaccgccgtc
     1561 cccgacaact actgaaaagc ggagcggcgc agcgttctcg aattgcagca acgcgggaac
     1621 gtcatggccc ttcggcgtcg ccggcgaggc tctcagatat gcggcgaggt agccctgcgg
     1681 cccgtgcttc tggcagtgca acgctataag cggcgcgata ttccctggat gcggcggcga
     1741 ttggatcatc gcgtcggtcg gcgcggcatg gggcgccggc ctttccacca cgacgatcca
     1801 gttcgaactt tccgtcgttt cggacagcgc ccatgccggg attgccgatc ctgcaacgaa
     1861 aaaagcggct aagcagcccg cggccttacg gaaaagatga cgggaccttt gatcccgact
     1921 tccttgtcga tcagccgcgc cgagagctgg ctgcgatcga aggcggcggc aagcaaatag
     1981 ggcggctcga atagtcggaa aaccgtctcc gctttcatgc ggctccagtc gtaaagctgg
     2041 gcgcagacca cgtcgccgcg ctttggcggc tcgttcaagt cgacgatcag aatgtcgccc
     2101 ggcagatagc cgatcgcttc gatggcgtgc gagcgcagtc gccagacggt caagctgttc
     2161 ttgctggcgg ttagcgcctc gatccgttcg gctagctccg ctgatgcgcg gctcgcgtcg
     2221 aaagcgtccg cctcgggctc gccgaaacct gatggcggcg cgccgcctgc gccaaatcgt
     2281 atgcccgtca ctctttccac cgccgcgatc gtccgcgccg aaagcgcgtt cgcgtgcgtc
     2341 gggttgttga gaaagcgcgt cagcgtcgtc gacgccaaat gagcccgttt cgccagcgtt
     2401 gtcgggggaa ttcccgtttg agcgatcacg ctttccaacc atgcgcgctg ctctattcga
     2461 gggtccattc gatagagcaa gcacgaaccc ccgacgcacg cagccagcat ctttcgagcc
     2521 caaggctctt gacaggctgc attaatgcag ggtcaattgc aacagaggtt caagtgattt
     2581 ggcctctgtt tgttcctgtg gctgtcgatt ttttgttcgc gttccccgac ttccaactgt
     2641 ggccgaggca tgacgctgga ccaaatcgac gccaggcggc gcgctgccgg cttgtccatg
     2701 agcgagcttt gccggcgggc gggcatcaat acgtccacct atcagcgaac gcggcagggc
     2761 aagggcggca attataggag gacgctggag cggctcgccg cggcgctcga aaatccgaaa
     2821 gccttaagcc ttagtcaatt gcggcgcggg tcgccggaga aagaaaccgc cctgatccgg
     2881 gcgaccttcc gcggctgggc ggcgcatttt gcgcgcgaga ccg
//
LOCUS       DATGJW010000414         3229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_808109, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000414 DATGJW010000000
VERSION     DATGJW010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3229
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_808109"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..485
                     /locus_tag="VKU03_12455"
     CDS             <1..485
                     /locus_tag="VKU03_12455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007197432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72124.1"
                     /translation="AWERVLSVNVAGLFQLTRDAAPLLFKAATADRPANVINIGSVMG
                     TVTQSGSAYSYSASKAAVHHLTRILAAEFAGRFVTVNALAPGPFKTKMTAFALGRAES
                     AAATSAGVPMKRVGRDEDIAGTVLYLTGAGGAYTTGAIIPIDGGCSVVPPPPLFPGEH
                     "
     gene            485..988
                     /locus_tag="VKU03_12460"
     CDS             485..988
                     /locus_tag="VKU03_12460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011926105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HLJ72125.1"
                     /translation="MSEPQATPSLTIADLIARIGTETGLSRWFTIDQKRIDAFAEATE
                     DHQFIHVDPEAARATPFGGTIAHGFLTLSMLSALGYDALPRPAGVAMGVNYGFEKLRF
                     VAPVPAGKRIRGRFVLQAVTQRGPLEYQLRNKVTVEIEGSDKPALVAEWLTMYFMKPD
                     HAKAEAQ"
     gene            985..1905
                     /locus_tag="VKU03_12465"
     CDS             985..1905
                     /locus_tag="VKU03_12465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711414.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72126.1"
                     /translation="MTITFDGRVAIVTGAGGGLGRSHALGLAARGAKVVVNDLGRGGE
                     PPETSLAVVEEIEEAGGVAMADGADVSNAEQVAAMVERAEAEWGSVDILVNNAGILRD
                     KSFAKMDLAEFELVVKVHLLGSAICAKAVWNGMRERNYGRVVFTASASGLYGNFGQAN
                     YGAAKAGMIGLMNVLHLEGAKYNIRVNTLAPTAATNMTAGLITPEMAALMTPESVTPG
                     VLYLVSENAPSRVILGAGAGTFAVSYITETSGLFLPEVHRTPETIAERFSEIANPASA
                     RPLDNAFAQTFKFVEAAARRLGVSLPKQGG"
     gene            1909..3111
                     /locus_tag="VKU03_12470"
     CDS             1909..3111
                     /locus_tag="VKU03_12470"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HLJ72127.1"
                     /translation="MREAVIVSTARTPIGKAYRGAFNDTQAQALGGHVIAEAVKRAKV
                     DTNEIDDVVMGAALQQGSTGGNIARQCAIRAGLPTSIAGMSLDRQCSSGLMAIATAAK
                     QIIDDGMVVTVGGGLESISLVQNEHMNRYRVKDPWFEAHRSDMYMSMLETAETVAERY
                     QVTRDRQDEYALQSQQRTAAAQAAGRLDAEIVPLPTRMKVADKATGAVSDKDVTLRQD
                     EGARADTTLAGLKALKPVFADGLRIAAGSYITAGNASQLSDGASAAVLMEAKEAEKRG
                     LQPLGVYRGMAVAGCDPDEMGIGPVFAVPKLLRTHGLKVDDIGLWELNEAFAVQVIYC
                     RDRLGIPNDRLNVDGGAISIGHPYGMSGARMTGHALIEGKRRGVKYVVVKMCVGGGMG
                     AAGLFEVA"
BASE COUNT          590 a         1027 c         1070 g          542 t
ORIGIN      
        1 aagcctggga gcgcgtgctg tccgtcaatg tcgccgggct ctttcaattg acccgcgacg
       61 ccgcgccgct tctcttcaag gccgcgaccg ccgacaggcc ggcgaatgtg atcaatatcg
      121 gctccgtcat gggcacggtc acgcaaagcg gcagcgccta ctcctattcg gcgtcgaaag
      181 cggcggtgca tcatctgacg cgcattctgg cggcggaatt cgccggccgc ttcgttacgg
      241 tcaatgcgct ggcgccgggc ccgttcaaga ccaagatgac cgccttcgcg ctcggccgcg
      301 cggaaagcgc cgccgcgacc agcgccggcg tgccgatgaa gcgcgtcggc cgcgacgaag
      361 acattgccgg aacggtcctg tatcttaccg gggccggcgg cgcctatacg accggcgcga
      421 tcattcctat cgacgggggc tgcagcgtcg tgccgccgcc gccgctcttt ccaggcgaac
      481 attgatgagc gaacctcagg caacgccgtc cctcacgatc gcggatctca tcgcccgcat
      541 cggaaccgag accggcctct cacgctggtt cacgatcgat cagaagcgca tcgatgcttt
      601 cgccgaggcg acggaggatc atcagttcat tcacgtcgat ccggaggctg cgcgcgcgac
      661 gcccttcggc ggaacgatcg cgcatggctt tctgacgctg tcgatgctct cggcgctggg
      721 atatgacgcg ctgccgcgtc cggccggcgt ggccatgggc gtcaattacg gtttcgagaa
      781 gctccgcttc gttgcgccgg tgccggccgg taaacgcatt cgcggtcgct tcgtcctaca
      841 ggcggtgacg caacgcgggc cgctcgagta tcaactgcgc aataaagtga cggtggagat
      901 cgaggggagc gacaaaccgg cgctcgtcgc cgaatggctg acgatgtatt ttatgaagcc
      961 ggaccacgca aaagcggaag cgcaatgaca atcacatttg acggccgcgt cgctattgtc
     1021 acgggcgccg gcgggggact tggccgctcg catgcgctcg ggcttgcggc ccgcggcgcc
     1081 aaagtggtcg tgaacgatct cggccgcggc ggcgagccgc ccgagaccag cctcgccgtc
     1141 gtcgaggaaa tcgaagaggc tggcggcgtc gctatggcgg atggcgccga cgtctcgaac
     1201 gccgagcaag tcgccgcgat ggtcgagcgc gccgaggccg aatggggaag cgtcgatatt
     1261 ctcgtcaaca acgccggcat tctgcgcgac aaatccttcg ccaagatgga tctcgccgaa
     1321 ttcgaactcg tcgtgaaagt ccacctcctt ggctcggcga tttgcgccaa ggcggtgtgg
     1381 aacggcatgc gggagcgcaa ttacggccgc gtggttttca cggcctcggc ctcgggtctc
     1441 tatggcaatt tcggccaggc caattacggg gccgccaaag ccggcatgat cggcctgatg
     1501 aacgtactcc acctcgaagg ggcgaaatat aatatccgcg tcaatacgct cgccccgacc
     1561 gcggcgacca acatgaccgc cggcttgatc acgccggaaa tggcggcgct gatgaccccg
     1621 gaatcggtga cgcccggcgt cctgtacctt gtgagcgaga acgcgccctc gcgcgtcatt
     1681 ctgggcgctg gcgcagggac cttcgcggtg agctatatta ccgagacctc cggccttttc
     1741 ctgcccgagg ttcatcgtac gccggagacg atcgccgagc gtttctcgga aatcgccaat
     1801 ccagcgagcg cgcggccgct cgacaatgcc tttgctcaaa cgttcaaatt cgtcgaagcg
     1861 gcggcgcgca ggcttggcgt ctcgctgccg aaacagggag gataaatcat gcgcgaagcg
     1921 gtcattgtct cgacggcgcg taccccgatc ggcaaggcct atcgcggcgc gttcaacgac
     1981 acgcaagccc aggccttggg cggccatgtc atcgccgaag cggtgaagcg ggccaaggtc
     2041 gacacgaacg agatcgacga cgtcgtcatg ggcgcggctt tgcagcaggg ttcgaccggc
     2101 ggcaatatcg cacggcaatg cgcgataagg gccggcctgc cgacgtcgat cgccggcatg
     2161 tcgctcgatc ggcaatgctc ctccggcctg atggcgatcg ccactgcggc caagcagatc
     2221 atcgacgacg gcatggttgt cacggtcggc ggcgggcttg aatcgatttc gctggttcag
     2281 aacgagcata tgaaccgcta tcgcgtgaaa gatccctggt tcgaggccca tcgctccgac
     2341 atgtatatgt cgatgctgga gacggccgaa acggtcgccg agcgctatca ggtgacgcgc
     2401 gaccggcagg acgaatacgc cctgcaatcg caacagcgca ccgccgccgc ccaggccgcc
     2461 ggccggctcg acgccgagat cgtacctctg cccacccgta tgaaagtcgc tgacaaggcg
     2521 acgggcgcgg tcagcgacaa ggacgtgacg ctcagacagg acgaaggcgc ccgcgccgat
     2581 acgaccttgg ccggcctgaa ggcgctgaag ccggttttcg ccgacggtct tcgcatcgcg
     2641 gcgggatcgt acattaccgc cggcaacgcc tcgcagcttt cggacggggc cagcgccgcc
     2701 gtactgatgg aggcgaaaga ggcggagaaa cgcggcctgc agccgcttgg cgtgtaccgc
     2761 ggcatggcgg tcgccggctg cgatcccgac gagatgggca tcggcccggt cttcgccgtg
     2821 ccgaagctgc tcaggacgca tggtttgaaa gtcgacgaca tcggattatg ggagctcaac
     2881 gaagccttcg cggtacaggt gatctattgc cgcgaccggc tcggcattcc gaacgaccgc
     2941 ctcaacgtcg acggcggcgc catttcgatc ggccatccct acggcatgag cggcgcccga
     3001 atgaccggcc acgcgttgat cgagggcaag cgccgcggcg tgaaatacgt cgtggtgaag
     3061 atgtgcgtcg gcggcggcat gggcgcggcc ggattgttcg aagtggctta attcccttct
     3121 cccgctgcgg gagaaggtgg cggcgaagca ggggatgaaa gtcggcggcg taccctcacc
     3181 cgcaagcggg agaggcgcgc gtggcgcgtc atcggcgtcg acggggttc
//
LOCUS       DATGJW010000415         5340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_185059, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000415 DATGJW010000000
VERSION     DATGJW010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5340
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_185059"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..347
                     /locus_tag="VKU03_12475"
     CDS             <1..347
                     /locus_tag="VKU03_12475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ72128.1"
                     /translation="AEDDNDMRRFLVKALQNAGYDVASFDNGLSAYNRLREEPFELLL
                     TDIVMPEMDGIELARRATELDPEIKVMFITGFAAVALNPDNNAPKDARILSKPFHLKD
                     LVSEVQRLLAAA"
     gene            complement(428..1741)
                     /locus_tag="VKU03_12480"
     CDS             complement(428..1741)
                     /locus_tag="VKU03_12480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017269912.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLJ72129.1"
                     /translation="MKIFRRRLFWKVYLTLLASLVAVATLIGAFWGIVGENMRPHWGA
                     LHLDLSDPTIPRRDSPPGAVAAAIGRLADESGADISLFDAEGALVAARGKPIALAESQ
                     AYRPPMRPPILRVDLPDGRAVLARLRTPPPHPRLRILSVVLIIAGGVAFAAFPVTARL
                     TRRLEGLRSGVERWGAGAHKLRVDEEGDDEVAVVARTFNAAAAQVEELLDSQKALLAN
                     ASHELRSPLARLRLAIELFSSQPSPQMQAEIVRNLGEIDRLVEEILLASRLDHAGAVA
                     AETAPIDLLGLAAEEAARFEARLAGAPGDARPVEIDGEATLLRRLIRNLLENAAKHGR
                     PPIEVAVTREAGKARIVVCDHGPGIAPAERERVFEPFYRPSGRGESSGGWGLGLALVR
                     QIAERHGGAAFCEALPGGGSRFVVELAVRREITGAGAGRAGARRA"
     gene            complement(1738..2436)
                     /locus_tag="VKU03_12485"
     CDS             complement(1738..2436)
                     /locus_tag="VKU03_12485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004434693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLJ72130.1"
                     /translation="MAERLLIIDDDSRLAAMVSDYLSKAGFLVDCRFTGRDGLAALDH
                     AAFDAVILDVMLPDLDGFEVCRRIRTRASTPILMLTARGEETDRIVGLEIGADDYLPK
                     PFNPRELQARLRAILRRGRGPAEEDAVLRFGRLEIDRASRQARVDGVAKTLTSFQFDL
                     LAAFAAHAGRVLNRDRLLDLVKGEDAEAFDRSIDVHVSRIRAAIEDDPKRPRRIITVR
                     GAGYVFARKQDEER"
     gene            complement(2565..3179)
                     /locus_tag="VKU03_12490"
     CDS             complement(2565..3179)
                     /locus_tag="VKU03_12490"
                     /inference="COORDINATES: protein motif:HMM:NF025173.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Spy/CpxP family protein refolding chaperone"
                     /protein_id="HLJ72131.1"
                     /translation="MTDTDHVPFPPEPPLRGPEEENGRRPFWRPRFFVLAGILAAGLA
                     LGAGGFAVAAGGLDHMGWGRGMRLAAIQRVVAHGLDSVGASAEQEAKVHDIIAGKFAD
                     IAPDPKESAALHKQALDLLAAPTIDRAAVEKLRADIVARFDAKSKAFVGVLLDVADQL
                     TPPQRAELSAHLQEMARRRAMGGWGGWRHGPPMGDGPDGGPDKD"
     gene            3581..3847
                     /locus_tag="VKU03_12495"
     CDS             3581..3847
                     /locus_tag="VKU03_12495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72132.1"
                     /translation="MNKLTLAAAAVVFMAATSGANAIGGPPHFNNDASAYWNGPPQFD
                     NNASTYWNGAAVQSDQTMIEGRAAYVAPSMRPSYEERGIIPTGR"
     gene            complement(4623..4856)
                     /locus_tag="VKU03_12500"
     CDS             complement(4623..4856)
                     /locus_tag="VKU03_12500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72133.1"
                     /translation="MTALGDETGRRHAAAPGAAGWLALAAAPTLAAMALLTYPRGAGM
                     DMMCSPSPLGGMAPMYVLTSAFHLAPWLKLIKR"
     gene            complement(4853..4978)
                     /locus_tag="VKU03_12505"
                     /pseudo
     CDS             complement(4853..4978)
                     /locus_tag="VKU03_12505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017988851.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 family protein"
BASE COUNT          887 a         1817 c         1833 g          803 t
ORIGIN      
        1 tggcggaaga cgacaacgac atgcggcgct tcctggtcaa ggcgctgcag aacgccggct
       61 acgacgtcgc ctccttcgac aacggcctat ccgcctataa ccggctccgc gaggagcctt
      121 tcgaattgct cctgaccgac atcgtgatgc cggaaatgga cgggatcgag ctcgcccgcc
      181 gggcgaccga gctcgacccg gaaatcaagg tgatgttcat caccggcttc gccgccgtgg
      241 cgctgaaccc cgacaacaac gccccgaagg acgcgcggat tttgtcgaag ccgttccacc
      301 tgaaggacct ggtcagcgaa gtgcagcgcc tgctggcggc ggcgtgagga ggaggcgggg
      361 acggacaggc cgtccccttg ccgcctggcg cgcgaaatca acgacgtgat cgtcgaggcg
      421 acgcgcgtca ggcgcggcgc gctcccgctc gccccgcgcc ggcgcccgta atctcccgcc
      481 tcaccgccag ctcgaccacg aaccggctgc cgcctccggg aagggcttcg cagaaggccg
      541 cgccgccatg gcgctcggcg atctggcgca ccaaggcgag cccgaggccc cagccgccgg
      601 aggattcgcc gcggccggac gggcggtaga acggttcgaa cacgcgctcg cgctcggcag
      661 gcgcgatgcc tggcccgtgg tcgcaaacga cgatgcgcgc ctttcccgcc tcgcgagtca
      721 ccgcgacctc gatcggcggc cggccgtgtt tagcggcgtt ttcaagcaga ttgcggatca
      781 ggcggcggag cagcgtcgct tcgccgtcga tctcgaccgg ccgagcatcg ccaggcgcgc
      841 cggcgagccg cgcctcgaaa cgcgccgcct cttcggcggc caagcccagc aggtcgattg
      901 gagcggtctc ggcggcgacc gcgccggcgt gatcgaggcg gctggcgagc aaaatttcct
      961 cgaccaggcg atcgatctcg ccgagattgc gtacgatctc cgcctgcatt tgcggcgaag
     1021 gctgggacga aaagagctcg atcgcgaggc gcaggcgcgc caggggcgag cgcaattcgt
     1081 ggctggcgtt ggcgagcaga gccttttgcg agtcgagcaa ttcctcgact tgcgcggcgg
     1141 cggcgttgaa ggtgcgcgcg acgaccgcca cctcgtcgtc gccctcctcg tcgacgcgca
     1201 gcttatgcgc gccggcgccc cagcgctcga cgcccgagcg cagcccttcg aggcgccgcg
     1261 tcagccgcgc cgtgaccggg aaggcggcga acgcgacgcc gccggcgatg atcagcacga
     1321 ccgacaagat gcgcagcctt ggatgcggcg gcggcgttct cagccgcgcc agaaccgcgc
     1381 ggccgtccgg aaggtcgacg cgcaggatgg gcggccgcat gggcggacga taggcttggc
     1441 tctcggccag ggcgatcggc ttgccccgag cggcgacgag cgccccttcg gcgtcgaaaa
     1501 gcgatatgtc ggcgccgctt tcgtccgcga gccgcccgat cgccgcggcg accgcgcctg
     1561 gcgggctgtc gcgccgtggg atcgtcgggt cggaaagatc aagatgaagc gccccccaat
     1621 gcggacgcat attttcgccg acgatccccc aaaacgcccc gatcagcgtc gcgacggcga
     1681 cgaggctcgc cagcaaggtc agatagactt tccagaacag gcggcggcga aagatcttca
     1741 tctctcttcg tcctgcttcc gggcgaagac gtaccccgcg ccgcgcacgg tgatgatgcg
     1801 gcgcggccgc ttaggatcgt cctcgatcgc ggcgcggatg cgtgagacat ggacgtcgat
     1861 cgaccggtcg aacgcctcgg cgtcctcgcc cttgacgagg tccaacagcc ggtcgcggtt
     1921 gagcacccgg ccggcatgag ccgcaaaggc ggccaggagg tcgaactgaa agctggtcaa
     1981 ggtttttgct acgccatcga cgcgcgcctg acgcgaggcg cggtcgattt cgagccggcc
     2041 gaagcgcagc acggcgtcct cttcggccgg cccgcggccg cgccgcaaaa tggcgcgcaa
     2101 gcgcgcctgc aattcgcgcg ggttgaacgg cttgggcagg tagtcgtcgg cgccgatctc
     2161 cagcccgacg atgcggtccg tctcttcgcc gcgcgccgtc agcatcagga tcggggtcga
     2221 cgcgcgagta cgaatcctcc ggcaaacttc gaacccgtcg agatcgggca gcatcacgtc
     2281 gagaatcacc gcgtcgaacg ccgcatggtc gagcgcggcg agcccgtcgc ggccggtgaa
     2341 gcggcaatcg acgaggaagc cggccttgct gagataatcg gagaccatgg cggccagcct
     2401 cgaatcgtcg tcgatgatca ggagccgctc cgccatgatg ctggtttcat ttggttgcgt
     2461 ataaatggct gttgtcgcgg cttaccctca tccgatcctt cgcgccgcca tccgaagggt
     2521 tgtagccgcg gtcgccgacc gcggctacag ctcgatgacg gcttctaatc cttgtccggg
     2581 ccgccgtcgg ggccgtcgcc catgggcggg ccgtggcgcc agccgcccca tccgcccatc
     2641 gcccggcgac gcgccatttc ttggagatgg gcggaaagct cggcgcgctg cggcggcgtg
     2701 agctggtcgg cgacgtcgag aagaacgccg acaaaggcct tggatttggc gtcgaaccgc
     2761 gcgacgatgt cggcgcgcag cttctcgacc gccgcgcggt cgatggtcgg cgcggcgaga
     2821 agatcgagcg cctgcttatg cagcgccgcg ctctccttgg ggtccggcgc gatgtcggcg
     2881 aatttgccgg cgatgatgtc gtgaaccttg gcttcctgct cggcgctggc gccgacggaa
     2941 tcgagaccat gcgcaaccac gcgctgaatc gcggcaaggc gcatgccgcg cccccagccc
     3001 atatggtcga gcccgcccgc ggccacggcg aagccgccgg cgccgagcgc aaggccggcg
     3061 gcgagaatcc cggccagcac gaaaaagcgc ggtcgccaga aggggcgacg gccgttctct
     3121 tcttccggcc cgcgcaaggg cggctcgggc gggaatggga cgtgatcggt atcggtcatg
     3181 acggtctcct tcgtccgcgg cggggcgccg cgtggcgaag gaggtaacgg gcgaagattt
     3241 cgcgcgatgc cgccgaatgt gaagaagcgt gaatgaattc gggcgctttg gcggtcggcg
     3301 ttacaccttc ggacgactat gacaatgtgt cccggcactc ccatcgccgt gctcccgtct
     3361 ggatcgggac cggcgcggtc gcgacgaagc gtccacgacg aagcaaaaga gcggccggag
     3421 ccgatcgacg gggcccggcc gacgcggccg ctgacccgat acgggtacga acagcactca
     3481 ctttagcgtg atcgccgggc tgaaacccac gccgaaggcg cgcgtaactt tagcacggag
     3541 gaggcggaac gggtcgcctc ccgcgacaag gactcccaca atgaacaagc tcactctcgc
     3601 cgccgcggct gtcgttttca tggcggccac ttctggcgcc aacgccatcg gagggccgcc
     3661 tcacttcaac aacgacgcct cggcctattg gaacgggccg cctcagttcg acaacaacgc
     3721 ctcaacttat tggaacggcg cggcggtaca aagcgaccag accatgattg aaggtcgcgc
     3781 cgcctatgtc gcgccgtcta tgcggcccag ctacgaagag cgcgggatca ttcccaccgg
     3841 ccgctgatcg ccgccagagt aactgaagcc gttcggccgc cgtcggaggc gaactcaaga
     3901 cctgaggagt ccgccgaacg cggtatcgag gcgagcatga gccggacgga cagtccctgc
     3961 gacaacgcca aagcgcagaa cgcacgaaga cgctgaaacc gaagaggcga acggcgaatt
     4021 tcgccggacc tcgacggcgc tcgccgtcgc atcggcgcct ccgttgagga aatctacaac
     4081 gccgaccttc actcgacgct cggctgcgct tcgtcgcttg cgttcgggca agccttccgc
     4141 cttgtcgctg aattaagctg tggagtgaag cggaacggtg agacgtacgg agaggggctg
     4201 gtcggttatc ctccgccgcc gcggccaggc gacaagcccc tctccgtacc tctccccgct
     4261 tcgcggggag agggggccca ggcgccgcgc tcattctcca gtccggtaag gcgtcggcgt
     4321 tttatcagtc cagtcagaac gccaaatgga aggcgctcgt cagcatttcg gtggcatttc
     4381 ggtgacggtg cattcaatag cttttccgcg cctgaatggc gccgcgccgt ctgccggcgc
     4441 tccatggcga agcggggcgt cgaagccccc tctccccgtg gagcggagcg gaacggggag
     4501 aggtacggag aggggctggt cggttatcct cagccgccgc ggccaggcga caagcccctc
     4561 tccgcccgct tcgcggggag agggggccca ggcgccgcgc tcattctcca tccggtaagc
     4621 cgtcagcgtt ttatcagctt cagccagggc gccaaatgga aggcgctcgt cagcacgtac
     4681 atgggggcca tgccgccgag cggcgagggc gagcacatca tgtccatgcc tgcgccgcgc
     4741 ggataggtca gcagcgccat ggcggcgagg gtcggcgcgg cggcgagggc gagccagccg
     4801 gccgcgccgg gcgccgccgc atggcggcgt ccggtttcgt ctccgagggc ggtcattgcg
     4861 ccgcctgcgc gtagctgtcg tggtgacgcc accagacgcc ctcctcattg cgtcccttcg
     4921 gggcgcggtc gagccattgg tacatgcccc agagcccgtc caggccgcgg gcataggtcg
     4981 gaagtcgaac tggcgctcgc cactctcgac gcgccggcgg cgcgggtcgt cgcgcgcggc
     5041 gaggcgcgcc ctcttctgct cgagcaagtc gaggcgctgc tcgcctcgaa ggcgctgatc
     5101 atcgacgcct ccgccggccc aggcatgggg gcggcgcgcc catgagagcc gggcgcgcga
     5161 attagactag gcgcgctgga ggcgcaacgg gcgtccccga gccggccgcg cgcgcaaatc
     5221 acaaatgagc gatgttaaag cgcgcgacaa ccgccgttcg cgctccgcgt ccgcgccgct
     5281 cttgacgggg gcgctcggcc cccttatctc ccgcccagct ttagcactcg ccggttttga
//
LOCUS       DATGJW010000416         7261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1218772, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000416 DATGJW010000000
VERSION     DATGJW010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7261
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1218772"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2923)
                     /locus_tag="VKU03_12510"
     CDS             complement(<1..2923)
                     /locus_tag="VKU03_12510"
                     /EC_number="2.7.7.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012330258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008882 - [glutamate-ammonia-ligase]
                     adenylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional [glutamine synthetase]
                     adenylyltransferase/[glutamine
                     synthetase]-adenylyl-L-tyrosine phosphorylase"
                     /protein_id="HLJ72134.1"
                     /translation="MTGAALAERLAAAPRPAETAAAKRRLAGLVAEAAPLAPLVERKR
                     TRDLLRGVADHSPYLWGLISEDPARLARLLGEAPEAALDRLVAAISARRDAEESELMR
                     ALRLAKREAALLIALADLGGVWDVVQVTEALSRFADAAVSAALRFLLRDYARAGRLIL
                     DPDAPDIERDSGLVVLALGKHGGRELNYSSDVDLVVFFDENAKSPPAGSEPGPLFARL
                     TKQLARLLQEHTGDGYVLRVDLRLRPDPGATAVAMAISSAYTYYENLGQNWERAAMIK
                     ARPVAGDKALGEKFLRDLSPFIWRKYFDYASIADVHAMKRQIHAVHGHEEVTAAGHNV
                     KLGRGGIREIEFFVQTQQLIFGGRRPRMRGARTLDMLAELKADGWVSAEAERELSTAY
                     QYLRRIEHRLQMIADEQTQRLPEDADAFARFAKFCGYDGPERFSDDLIGHLRRVEKHY
                     ARLFEDAPGLDANAGSLVFTGVVDDPETLQTLRRMGFHDPERAAETIRGWHFGRRAAI
                     RSPRAREALTEVTPGLLEAFAASGDPGAALATLDSALARMIAAVELFSILRSNADVRQ
                     LFADILGGAPRLANVIATRPHVLDAVIDPARAAAFDERAARGRLEAFLAGASTFESVL
                     DRVRDFAAEDMFLIGVRLFSGALDPDRAGRAYSALAEAIIGALLDRVGELFAGDHGRV
                     PGGRVAIIGMGKLGSREMTATSDLDLILVYDFPSGAESDGARPLPAVLYYTRLTQRLI
                     AALTAPTKAGRLYDVDLRLRPSGRKGPLATQFQGFAAYQRDEAETWEHMALTRARAIA
                     GDAEFGEEVEKAIGEILRKTRDRAVLAKDVRDMRALIAQEKGDDDGWNLKLAAGGLLD
                     IEFVAQFLVLAEAGEHPDVRDVSTRAVIAKAGAAGLLTVEQARALTEAHRLFTDATQL
                     MRLSVDGPFDPAKAAGGVTRRIAAAAELPDIGALEGAIREARGEVREVFEAILKPART
                     PR"
     gene            complement(2920..4413)
                     /locus_tag="VKU03_12515"
     CDS             complement(2920..4413)
                     /locus_tag="VKU03_12515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ72135.1"
                     /translation="MIALGKLFRTTAFKLSLVFMALSAVGAGVVLSIVAFEVFALVDQ
                     ESRQTIAAEADGLAEQYAQGGIRRLGYVIEQRTREPGSSLYLLTNFAGETLAGNIERL
                     PPGVLDKPGFVETSYETIDDSTFGRRALARIFVLPGGFRLLVGQDLRDRARIGHVMVR
                     SLAVSLIGLAVLGALGGLFVARRVLRRIDAMSNASRAIMAGDLSERLPVSGSGDELDR
                     LAENLNDMLKRIGELMLGLKEVSDNIAHDLRTPLTRLRNHAEAALRLEGDVKDYRAAL
                     ERTIEESDGLIRVFNALLMIARAEAGADRSALSDFDVGEAARSVVELYEPAAEEAGAT
                     LKAEIEPGLIVRGNRELLGQSIANLLDNALKYGAAEPVLALAPPTAPAEEIRTGPPPA
                     AEVQLVVRRAGEFVEIVIADHGPGIAPADRARVFERFVRLEGARARAGSGLGLSLAAA
                     VAHLHGGAVRLEDNRPGLRVVISLPARPALIEPAFETAPAANAAALS"
     gene            complement(4397..5080)
                     /locus_tag="VKU03_12520"
     CDS             complement(4397..5080)
                     /locus_tag="VKU03_12520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407552.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLJ72136.1"
                     /translation="MRILIIEDDRDAAAYLVKAFREVGHVADHAADGVTGYDLAREGG
                     YDVAVVDRMLPKMDGLSLIGALRAQNVDTPILILSALGQVDDRVKGLRAGGDDYLAKP
                     YAFSELLARVDALARRRPKGATEETVYRIADLELDRLSHKVTRGGAEIPLQPREFRLL
                     EYLMQHAGKVVTRTMLLESVWDYHFDPQTNVIDVHISRLRAKIDKDFDPPLLHTVRGA
                     GYMIRDSAR"
     gene            complement(5201..6742)
                     /locus_tag="VKU03_12525"
     CDS             complement(5201..6742)
                     /locus_tag="VKU03_12525"
                     /EC_number="3.4.21.107"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Do family serine endopeptidase"
                     /protein_id="HLJ72137.1"
                     /translation="MRPLHSSRFGFRTLRGALLGAVALTAIGGLTFEGLSSYPALAQA
                     SAIQPTQPSGPASFADIVDHVKGAVVSVKVTMRDSDQASSDLGPGGPLDRFFRQFGQG
                     VDPFGDEGDSPIPRIPHHHPHHLEMAQGSGFFISPDGYIVTNNHVVEHATEVTVTTID
                     GKTIPAKVIGTDPKTDLALLKVKEGDNYPYVKFADKAPRVGDWVIAVGNPFGLGGTVT
                     AGIVSARGRDIGSGPYDDFLQIDAPVNRGNSGGPSFNNQGEVVGVNTAIFSPSGGSVG
                     IGFAIASDVVQNVVQQLKTNGMVTRGWLGVEIQPVNQDIADSLGLKQTQGALVASAQK
                     NSPAAEAGVKPGDVITKVDGEPVANPRELARKIAALGPKKTVDLTVVRNDQTKDISVT
                     LGQMANEKQASNEQPSSNDNTAQSDDAAQKEMAKLGLTLAPEKGGKNGVVVADVDPDS
                     AAAQKGLQQGDVILDAGGKPVTKASDVTAAIAAAKSEGRKAVLLRVKSGDNVHFVALS
                     TQAAT"
     gene            complement(6896..>7261)
                     /locus_tag="VKU03_12530"
     CDS             complement(6896..>7261)
                     /locus_tag="VKU03_12530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008966200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HLJ72138.1"
                     /translation="RARALSAELRCMVCQNESIDDSDASLAHDIRVLIRERLAKGESE
                     DAIKAFLVSRYGEFILLKPPLQRDTLLLWGAPPLLLIGGAVFLGVAMRRKSRGGLAPA
                     ALTSAEEQRVAALLDDSKI"
BASE COUNT         1251 a         2403 c         2445 g         1162 t
ORIGIN      
        1 ggcgaggggt acgagccggc ttcagaatcg cctcgaacac ttcgcgcacc tcgccgcgcg
       61 cctcgcggat cgccccctcc agcgcgccga tatcgggcag ttccgccgcg gcggcgatgc
      121 gccgcgtcac cccgccggcg gctttcgccg gatcgaacgg cccgtcgacc gaaagccgca
      181 tcaattgcgt cgcatcggta aacagtcgat gggcttcggt cagcgcgcgg gcttgctcga
      241 ccgtcagcaa gccggccgcg ccggctttgg cgatcaccgc gcgggtcgac acgtccctga
      301 cgtcgggatg ttcgccggct tcggcgagga ccaggaactg cgcgacgaat tcgatatcga
      361 gcaagccgcc ggcggcgagc ttcaagttcc agccgtcgtc gtcgcctttc tcctgcgcga
      421 tcagcgcgcg catgtcgcgc acgtctttgg cgagaaccgc gcgatcgcgc gtcttgcgca
      481 agatctcgcc gatcgccttt tcgacctctt cgccgaattc ggcgtcgccg gcgatggcgc
      541 gcgctctggt cagcgccata tgctcccagg tctcggcctc gtcgcgctga taggcggcga
      601 agccctgaaa ctgcgtcgcc aacggcccct tgcggccgga cggacgcaag cgcaggtcga
      661 catcatagag gcgtcccgcc ttggtcggcg ctgtgagcgc ggcgatcagg cgctgcgtca
      721 ggcgcgtata gtagagtacg gccggcaaag gtcgcgcgcc gtccgattcc gcgccgctcg
      781 gaaagtcgta aaccagaatc agatcgagat cggaggtcgc cgtcatctcg cgcgagccga
      841 gcttgcccat gccgataatc gcgacgcgcc cgccgggaac gcgcccatgg tcgccggcga
      901 aaagctcgcc gacgcggtcg agcagcgcgc cgataatcgc ctcggccagc gcgctatagg
      961 cgcggccggc ccggtcggga tcgagcgcgc cggaaaaaag ccgtacgccg atcaagaaca
     1021 tgtcctcggc cgcgaagtcg cgcacgcgat cgaggacgct ctcgaatgtg cttgcgccgg
     1081 cgagaaaggc ttcgagccgc cctctggcgg cgcgttcgtc aaaagcggcg gcgcgcgccg
     1141 gatcgatcac ggcgtccagc acatgcggcc gcgtcgcgat gacattggcg aggcgcggcg
     1201 cgccgcccag aatgtcggcg aaaagctgcc ggacatcggc gttggagcgc aggatcgaaa
     1261 agagctcgac cgccgcaatc atccgggcga gcgcggaatc gagcgtcgcg agggcggcgc
     1321 ccggatcgcc cgaggcggcg aaggcttcga gcaggcccgg cgtcacttcg gtcagcgctt
     1381 cgcgcgcgcg gggactgcga atggcggcgc ggcggccgaa atgccagccg cggatcgtct
     1441 cggccgcgcg ttcgggatcg tgaaagccca tccgccgcag cgtttgcagc gtctcgggat
     1501 cgtcgacgac gccggtgaag accaggctgc cggcattcgc gtcgagcccc ggcgcgtctt
     1561 cgaagagccg cgcataatgc ttctcgaccc gccgcaaatg gccgatcagg tcgtcgctga
     1621 agcgctccgg cccgtcatag ccgcagaatt tggcgaagcg ggcgaaggcg tcggcgtcct
     1681 cgggcagcct ttgcgtctgc tcgtcggcga tcatctgcaa acgatgctcg atccggcgca
     1741 aatattggta agcagtcgag agctcgcgct cggcctcggc gctcacccaa ccgtcggctt
     1801 tcagctcggc gagcatgtcg agcgtgcggg cgccgcgcat gcgcggcctg cgtccgccga
     1861 aaatgagctg ctgcgtctgg acgaagaact cgatctcgcg aatgccgccg cggccgagct
     1921 tgacgttatg gcccgccgcc gtcacctctt cgtggccgtg aacggcgtga atctgccgct
     1981 tcatcgcatg aacgtcggcg atcgaggcgt aatcgaaata tttacgccag atgaacggcg
     2041 agagatcgcg caagaacttt tcgccgagcg ccttatcccc ggcgaccggc cgcgccttga
     2101 tcatcgccgc gcgctcccag ttctggccga gattctcata gtacgtataa gcggaggaga
     2161 tcgccatggc gaccgcggtc gcgcccggat cgggccgcag ccgcaagtcg acgcgcagga
     2221 catagccgtc gccggtatgc tcctggagca gccgcgccaa ttgcttggtc agacgcgcga
     2281 aaagcgggcc gggctcgctt ccggccggcg gactcttggc gttctcgtcg aaaaagacga
     2341 cgagatcgac atcgctcgaa tagttgagct cgcgcccgcc atgcttgccg agcgccagca
     2401 cgacaaggcc gctgtcgcgc tcgatgtcgg gcgcgtcagg gtcgagaatc aggcgtccgg
     2461 cccgcgcata atccctgaga aggaagcgca gagcggcgga gaccgccgcg tcggcgaagc
     2521 gcgacaaggc ctcggtgact tggacgacat cccacacgcc gccaagatcg gcgagcgcga
     2581 tgagcaaagc cgcctcgcgt ttggcgagac ggagcgcgcg catcaattcg ctttcttctg
     2641 cgtcgcggcg tgcgctgatc gccgcgacca ggcgatccag cgccgcctcc ggcgcctctc
     2701 cgagaaggcg cgccaggcgc gccgggtctt ccgagatcag cccccacaga tagggcgagt
     2761 ggtcggcgac gccgcgcagc aagtcgcgcg tcctcttccg ctcgaccaag ggcgcgagcg
     2821 gcgccgcctc ggcgacgagg ccggcgagcc ggcgcttcgc ggcggcagtt tccgccgggc
     2881 gcggcgcggc ggcgagccgc tcggcgagcg cggcccccgt cacgaaagcg ccgccgcatt
     2941 ggcggcgggc gccgtttcga aggccggctc gatcagcgca ggacgcgccg gcaggctgat
     3001 gacgacccgc aggcccggcc gattgtcctc gagccgaacc gcgccgccat gcagatgggc
     3061 gaccgccgcg gcaagcgaca ggccgaggcc agagccggcg cgcgcgcgcg cgccttccag
     3121 gcggacgaag cgctcgaaga cgcgagcgcg gtcggccggc gcaatgcccg gcccatgatc
     3181 ggcgatcacg atctcgacga actcgccggc gcggcgaacg acaagctgca cctcggccgc
     3241 cggcggcggc ccggtccgta tctcctcggc cggcgcggtt ggcggggcaa gcgcgaggac
     3301 gggctccgcc gcgccgtatt tcagcgcgtt gtcgagcaga ttggcgatgc tctggccgag
     3361 cagctcgcga ttgccgcgca cgatcaatcc gggctcgatc tctgccttga gcgtcgcgcc
     3421 ggcttcttcg gcggccggct cgtaaagctc gacgacgctg cgcgccgcct cgccgacatc
     3481 gaaatcgctc agcgccgagc gatccgcccc cgcttcggcg cgggcgatca tcagcagggc
     3541 gttgaagacc cggatcaagc cgtccgattc ctcgatcgtc cgttcgagcg cggcgcgata
     3601 gtccttgacg tcgccttcga ggcgcagcgc cgcctcggcg tggttgcgca gacgcgtcaa
     3661 cggcgtgcgc aaatcgtgcg cgatattgtc tgagacttcc ttgaggccga gcatcaattc
     3721 gccgatccgc ttgagcatat cattgagatt ctcggcgagc cggtcgagct cgtcgcccga
     3781 gcccgagacc ggcaggcgct cgctgagatc gccggccatg atcgcgcgcg aggcgttgct
     3841 catcgcatcg atccgccgca gcacccgccg cgcaacgaaa agcccgccca aggcgccgag
     3901 caccgccaag ccgatcagcg acaccgccag cgagcgcacc atcacgtgac cgatgcgggc
     3961 gcgatcgcgc aagtcctgcc ccacaagcag ccgaaagccg ccgggcagca cgaagatgcg
     4021 ggcaagggcg cgccggccga aggtcgaatc atcgatcgtc tcatacgagg tctcgacgaa
     4081 gccgggcttg tcgagcacgc cgggcggcag acgctcgata ttgccggcca gcgtctcgcc
     4141 ggcgaagttg gtcaacagat agagagagga gccgggctcg cgggtgcgct gctcgatcac
     4201 gtagccgagc ctacggatgc ctccctgggc gtattgctcg gcaaggccgt ccgcctcggc
     4261 ggcgatggtc tgacgcgatt cctgatcgac cagcgcgaag acttcgaaag cgacgatgct
     4321 gaggaccacg ccggcgccga cggcgctcag cgccatgaag acgagcgaca gcttgaacgc
     4381 cgtcgtgcgg aagagcttac cgagcgctat cacggatcat gtatcccgcg ccgcgcaccg
     4441 tatgaagcag cggcggatcg aaatccttgt cgatcttggc gcgcaaccgc gaaatatgca
     4501 cgtcgatcac attggtctgc ggatcgaaat gatagtccca gacgctctcc agcaacatgg
     4561 tcctggtgac caccttgccg gcatgctgca tcagatattc cagcaagcgg aattcgcgcg
     4621 gctgcaaggg gatttcggcg ccgccgcggg ttactttatg cgaaagacgg tcgagttcga
     4681 gatcggcgat gcgatagacc gtctcctcgg tggccccttt cggccggcgc cgcgccagcg
     4741 catcgacgcg cgcgagcaat tcggaaaagg cgtagggctt ggcaagatag tcgtcgccgc
     4801 cggcgcgcag gcccttgacg cggtcatcga cctggccgag cgccgaaaga atgagaatgg
     4861 gcgtgtcgac attctgcgcc cgcaaggcgc cgatgagcga caaaccatcc atcttcggca
     4921 acatgcgatc gacgaccgcg acgtcatagc cgccttcccg cgcgagatcg tagccggtca
     4981 cgccgtcggc ggcgtgatcg gcgacatggc caacctcgcg gaacgccttg acgagatagg
     5041 cggccgcgtc gcgatcgtcc tcgatgatca aaatccgcat ggaggacata tagcggctcc
     5101 caaacaaaaa cggcaggcca ggctggaagc ccgacctgcc gccgcttcac ccggctgcgc
     5161 gccaggggcg ggcgcgcgct gccggaggct actcaagcgg tcaggtggcc gcctgggtcg
     5221 agagggcgac aaaatgcaca ttgtcgcctg atttcacgcg cagcagaacc gccttgcggc
     5281 cttcgctctt ggcggcggcg atcgccgcgg tcacatcgct cgccttggtc accggcttgc
     5341 cgccggcgtc gagaatgacg tcgccctgct gcaagccttt ctgcgcggcc gcgctgtccg
     5401 ggtcgacatc cgcgacgacg acgccattct tgccgccctt ttccggcgcc agggtcaggc
     5461 cgagcttggc catttccttc tgggcggcgt catcgctctg ggccgtgttg tcgttcgacg
     5521 acggctgttc gttcgacgcc tgcttttcat tggccatctg gccgagcgtc accgagatgt
     5581 ccttggtctg gtcgttccgc accacggtga ggtccacggt cttcttggga ccgagcgcgg
     5641 cgatcttgcg cgccaactcg cgcgggttgg cgacgggctc gccatctacc ttggtgatga
     5701 cgtcgccggg cttcacgccg gcctcggccg caggcgaatt cttctgggcg ctggcgacca
     5761 gcgcgccttg ggtctgcttc aagccgagac tgtcggcaat gtcctggttg accggctgga
     5821 tttcaacgcc gagccaaccc cgcgtcacca tgccgttggt cttgagctgc tgcacgacgt
     5881 tctggaccac gtcgctggcg atggcgaagc cgatgccgac gctgccgccc gacggcgaga
     5941 agatcgcggt gttgacgccg accacttcgc cttgattgtt gaagctcggt ccgccggaat
     6001 tgccgcgatt gaccggcgcg tcgatctgca ggaagtcgtc atagggaccc gagccgatgt
     6061 cgcggccgcg cgccgaaacg atgcccgccg tcaccgtgcc gccaaggccg aacggattgc
     6121 cgaccgcgat cacccagtcg ccgacgcgcg gcgccttgtc ggcgaatttc acgtaaggat
     6181 agttgtcccc ttccttgacc tttagcagag caagatcggt cttgggatcg gtgccgatca
     6241 ccttggccgg aatagtcttg ccgtcgatcg tcgttacggt tacttcggtc gcgtgctcga
     6301 cgacatggtt gttcgtcacg atatagccgt ccggcgagat gaagaatccc gacccttgcg
     6361 ccatttcgag gtgatgggga tgatgatgcg ggatgcgcgg gatcggcgaa tcgccctcgt
     6421 cgccgaacgg atcgacgcct tggccgaact ggcggaagaa tcggtcgagc gggccgcccg
     6481 gcccgaggtc ggagctcgcc tgatcggaat cgcgcatcgt gaccttcacc gaaacgactg
     6541 cgcctttcac atggtcgaca atgtcggcga atgaggcggg gccggacggc tgagtcggct
     6601 ggatggccga ggcttgggcg agggccggat aggaggaaag accttcgaac gtgagcccgc
     6661 caatggcggt taaagcgacg gcgccaagca gcgcgccccg taaagtacga aagccaaatc
     6721 gggaagaatg gagtggacgc ataatggtct ccttggtgaa gcggcgccag cgaggctcga
     6781 tccgacgttg tgcggcagag aatgaatcag cgccgtttac ggcgcgatgg cgcgaagatg
     6841 aatgtttgtt aaggcggggc gcgacgccgg ccgcctcaca attatttgaa aaagcttata
     6901 ttttcgagtc gtcgagcagc gcggcgacgc gctgctcctc ggccgaggtt aacgcggcgg
     6961 gcgcaaggcc gcctcggctt ttccggcgca tcgcgacgcc gaggaaaacc gcgccgccga
     7021 tgaggagcaa gggcggcgcg ccccaaagga gcagcgtgtc gcgctgaagc ggcggcttga
     7081 gcaggatgaa ttcgccatag cgcgagacga ggaaagcctt aatggcgtct tcgctttcgc
     7141 ctttggcgag gcgctcgcgg atcaggacgc gaatgtcatg cgccaacgag gcgtcggaat
     7201 cgtcgatcga ttcgttctgg caaaccatgc agcgcaattc ggccgaaagc gcgcgggcgc
     7261 g
//
LOCUS       DATGJW010000417         2645 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_81383, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000417 DATGJW010000000
VERSION     DATGJW010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2645)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2645)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2645
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81383"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..893)
                     /locus_tag="VKU03_12535"
     CDS             complement(300..893)
                     /locus_tag="VKU03_12535"
                     /inference="COORDINATES: protein motif:HMM:NF020965.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyloxyacyl hydrolase"
                     /protein_id="HLJ72139.1"
                     /translation="MLKFLGLAALGLIAASAMAPVALGADATPYGGLAAPAPASPLAD
                     WEVRGGLYAHDPLSPEAGSVDLNGELVGPKLWQSSDPLWSLLIPRPDIGTTISLGGKT
                     SNLWGGAAWNFDLTDRIFLSSTFGVGINNGKAGNTVPPGWNAVGCNWWFHESESLGYR
                     LTQNWSVMATIEHSSNAGLCHENRGLTNAGLRLGYRF"
     gene            complement(1081..>2645)
                     /locus_tag="VKU03_12540"
     CDS             complement(1081..>2645)
                     /locus_tag="VKU03_12540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404283.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF6537 domain-containing protein"
                     /protein_id="HLJ72140.1"
                     /translation="LVCEGCGDCGVKSNCVSVQPLETAFGRKRQIDQSACNKDFSCLD
                     GFCPSFVTVYGARLKKSAPSAAAPASPEPVARTLGDRPYSIVVTGVGGTGVVTIAAIL
                     GMAAHIEGKACGMIDMAGLAQKGGAVFSHVKLARRPEDIHAIRVAAGDADLILGCDLV
                     VSGAKKVLAALRGPETGFVVNSAEIYPGDFTRNPDFSLPAERIKRAIKAASGDGALFV
                     DATRAANALVGNAIGANMFMLGYAYQAGYVPLSAAAIRRAIELNGEAIAMNLAAFDWG
                     RAAYAEPQSIAAVGEPPARPAPAETLEEIVARRADFLTGYQNAAYAGRYRAMVARIEA
                     AERQRTPGLTGLAAAVARNLFKLMAYKDEYEVARLYTDGSFKKQVEATFEGDLRFEYH
                     LAPPLLALKDRRTGEARKMRFGGWMIQGFKALAALRFLRGSAFDPFGYLPERREERRL
                     IGEYEALCEELAARLSPENHALAVALASLPEKIRGFGHVKARAIAATAAEGEKLIARW
                     RSPAAPLQDAAE"
BASE COUNT          447 a          896 c          860 g          442 t
ORIGIN      
        1 tcgccgcgat cgggttcttc gttcagttcg tcgcgatcgt cgtctcggcg ccgccgcgcc
       61 tcaaggcgtt gcccgaggcg gcggagtaat cgaccgccgt gctgtagccg cggtcgcgga
      121 ccgcggctac ccctcccgct tgcgcagagg gcaacgcccg aagggccgga tgaaggccgc
      181 taagcttgcg ggttagcctt gaagggcgag cgcctttcga gtcccgcgcc gcccttcagc
      241 cagccgggtt tcgcgcggcc gccttctcac cgctttgcga cgcttcgcgg ggcgagggtt
      301 cagaaccgat agcccagcct cagcccggca ttggtcaggc cgcgattctc gtggcagagc
      361 ccggcgttgg acgaatgctc gatcgtcgcc atcacgctcc agttctgggt cagccggtag
      421 ccgagcgatt cggattcgtg gaaccaccaa ttgcagccga cggcgttcca gccgggcggg
      481 acggtgttgc cggccttgcc gttgttgatc ccgacgccga acgtagagct gaggaaaatg
      541 cggtccgtta aatcaaaatt ccaggcggcg ccgccccaga gattgctggt cttgccgccg
      601 aggctgatcg tcgtcccgat atccggccgg gggatcagca ggctccacag cggatcggaa
      661 gattgccaga gtttcggccc gaccagctcg ccgttgagat cgaccgagcc ggcttccggg
      721 cttaacggat catgcgcata aaggccgccg cgaacttccc aatccgccaa gggcgaggcc
      781 ggcgcggggg cggcaaggcc gccatagggg gtcgcatccg cgcccagcgc gaccggcgcc
      841 atggcggacg ccgcaattag gcccagcgcc gctaaaccga gaaatttcag caaatccgcg
      901 cccctccgcg actgagaaat cagtcgccac gtttcgttcg gcctgtccca cgcgcccacg
      961 gccggcttcc cccaagcgcc ggcaggcgcg cagcggcttt ccgctattcg caaacaggtt
     1021 tacaggcgat tcgccgccgc tggcaatacg cctgggctta ggctttcgag tcttggccga
     1081 ttattccgcc gcgtcttgca aaggcgccgc aggggagcgc cagcgggcga tcagcttctc
     1141 gccctccgcg gcggtcgccg cgatggcgcg cgccttgaca tggccgaaac cgcgaatctt
     1201 ttcgggcagg ctggccaagg cgaccgccaa agcgtgattt tcgggcgaaa gccgcgcggc
     1261 gagctcctcg caaagcgcct cgtattcgcc gatcaggcgt cgctcctcgc ggcgctccgg
     1321 caaatagccg aacgggtcga aagcgctgcc gcgcaagaag cgcaaagccg ccagcgcttt
     1381 gaagccttgg atcatccacc cgccgaagcg catcttgcgc gcctcgccgg tcctcctgtc
     1441 cttcagcgcg agaagcggcg gggcaagatg atattcgaag cgcagatcgc cctcgaaagt
     1501 cgcctcgacc tgttttttaa acgagccgtc cgtatagagc cgcgcgacct cgtattcgtc
     1561 cttgtaagcc atcaatttga agaggttgcg ggcgaccgcc gcggcgaggc cggtaaggcc
     1621 cggcgtccgc tggcgctccg ccgcctcgat tctcgcgacc atggcccgat agcggccggc
     1681 ataggcggcg ttctgatagc cggtcaggaa atcggcgcgg cgcgcgacaa tctcttcgag
     1741 cgtctcggcc ggcgcgggcc gcgccggcgg ctcgccaacg gcggcgatgg attgcggttc
     1801 ggcgtaagcc gcccgccccc aatcgaaagc ggcgagattc atcgcgatcg cctcgccatt
     1861 gagctcgatc gcccggcgga tcgcggcggc ggaaagcggc acatagcccg cctgataagc
     1921 gtagccgagc atgaacatat tcgcgccgat ggcgttgccg accagcgcat tggcggcccg
     1981 cgtcgcgtcg acgaacaatg cgccatcgcc gcttgccgcc tttatcgccc gcttaatgcg
     2041 ctcggcgggc agcgagaaat ccggattgcg agtgaagtcg ccgggataga tttccgcgct
     2101 gttgacgacg aagccggtct ccggcccgcg caaggcggcg aggacttttt ttgcgcccga
     2161 cacgacgagg tcgcagccaa ggatcagatc ggcgtcgccc gccgcgacac ggatcgcgtg
     2221 aatgtcctcc ggccgccgcg ccagcttgac atggctgaac accgcgccgc ccttttgcgc
     2281 gaggccggcc atgtcgatca tgccgcaggc cttgccctcg atatgggcgg ccatgccgag
     2341 aattgcggcg atcgtgacga cgccggttcc gccgacgccg gtgacgacga tcgaataggg
     2401 cctgtcgccg agcgtccgcg cgaccggctc gggcgaagcc ggcgctgcgg cgcttggcgc
     2461 gcttttcttc agccgggcgc catacaccgt cacgaaagac gggcaaaagc cgtccagaca
     2521 ggaaaagtcc ttgttgcagg ccgattggtc gatttgccgc ttgcgaccga acgccgtctc
     2581 caagggctgc accgagacac aattggattt gacgccgcaa tcgccgcagc cttcgcagac
     2641 gagct
//
LOCUS       DATGJW010000418         3573 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_490962, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000418 DATGJW010000000
VERSION     DATGJW010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3573)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3573)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3573
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_490962"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..490
                     /locus_tag="VKU03_12545"
     CDS             <1..490
                     /locus_tag="VKU03_12545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72141.1"
                     /translation="LKRLAANFERLRLPVEIAVADALSFEAPPFDAALLDAPCSATGT
                     IRRHPDVAWTKRASDIAALAALQAQLLDRAIALVKPGGRVVFCTCSLEPEEGEALIAA
                     LLRRNPDVRRLPVAADEVGGLAECVTEAGDLRTLPCHLPGPTPRLSGLDGFFAARLQR
                     TS"
     gene            607..2316
                     /locus_tag="VKU03_12550"
     CDS             607..2316
                     /locus_tag="VKU03_12550"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HLJ72142.1"
                     /translation="MSSQATAAITMRLVGLATRSYVERLARPFGALARRIASLGARRP
                     ERLAIAPRDIRTADPIAARDIYAGIFALAGRTGVAHGRSPFEIPPPSKGWAEALHGFG
                     WLRDLAAADTALSRANAQALVEDWLNVGARKGFAVESAIINDPRVAARRLMSWLSHSP
                     VILENADRAFYRRFARSLGGQAAPIERALAGGLKGEARLVAAIALTQTGLSLENAGTL
                     LRRAGRLLAAELSRQILADGGHVSRNPRVLVDALLDLLPTRQAFAARGLAPPEQLLNA
                     IDRMTPILRMFRHADGALANFNGMGVSEPETLATLLAYGDPGAAIMDAPYSGYQRLEA
                     GGSIAIIDVGKPPPWQFAQDSHAGCLSFEFSSDAHRIIVNCGAPPPDMSEARLAARAT
                     AAHSTLVVADHSSCRFAAPTGLEAFALGAALQGPSLVEAKRGADEDWLRVDAVHDGYA
                     RAFGVAHHRMLALARDGAQLCGEDRLAPSGKAQAAGETPIASRFHLHPDIAAESEGEA
                     AAAVLVTPSGQAWRFEAGGRDIAIEESIFFAAPGRPRRSSQLVVHATIAEAAPIVWSL
                     TRA"
     gene            2452..>3573
                     /gene="purH"
                     /locus_tag="VKU03_12555"
     CDS             2452..>3573
                     /gene="purH"
                     /locus_tag="VKU03_12555"
                     /EC_number="2.1.2.3"
                     /EC_number="3.5.4.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016844509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0003937 - IMP cyclohydrolase activity
                     [Evidence IEA];
                     GO_function: GO:0004643 -
                     phosphoribosylaminoimidazolecarboxamide formyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional
                     phosphoribosylaminoimidazolecarboxamide
                     formyltransferase/IMP cyclohydrolase"
                     /protein_id="HLJ72143.1"
                     /translation="MANDLARVGRALISVSDKKGLIALAGALAAHGVELVSTGGTAKA
                     LADAGYAVRDAAELTGFPEMMDGRVKTLHPGIHGGLLAVRENPAHEAAMLAHNIAPID
                     LLVVNLYPFEETLQRGRSFDEMIENIDIGGPAMIRGAAKNHSDVAVIVEPNQYASLIE
                     ELQRHDGATSLAFRRALAQRAYARTAAYDAAISNWLAGVTGEAAPPWRAFGGALIEAM
                     RHGENPHQAAGFYAGPEKRPGVATARQAQGKQLSYNNVNDTDAAFELVAEFDPTRVAA
                     VAIIKHANPCGVAEGETLVEAYRKALACDSVSAFGGIVALNRTLDADAARAITDIFTE
                     VIIAPDATAEAIAIVGAKKNLRLLLAGGLPDPRAAGLYAK"
BASE COUNT          527 a         1298 c         1172 g          576 t
ORIGIN      
        1 gctcaagcgt cttgccgcca atttcgagcg gctgcggctg cctgtcgaga tcgccgtggc
       61 cgacgcgctc agcttcgagg cgccgccctt cgacgcggcg ctgctcgatg cgccgtgctc
      121 ggcgaccggc acgatccgcc gccatcccga cgtcgcctgg acgaagcggg cgagcgacat
      181 cgccgcgctc gccgccttgc aagcccagct cctcgatcgc gccatcgcgc tggtcaagcc
      241 gggcggtcgg gtcgtcttct gcacctgttc gctcgaaccg gaagaaggcg aagcgctgat
      301 cgcggcgctg ctgcggcgca atcccgacgt acgtcgcctg ccggttgcgg ccgacgaggt
      361 cggcggtctc gccgaatgcg tcaccgaagc cggcgatctg cgcacattgc cgtgccatct
      421 gccggggccg acgccgcgcc tttccgggct cgacggcttt ttcgccgcgc ggctgcaaag
      481 gacgagttga ggcgaagccg tcgcgccgcg ccatcatccc cagcgaactt gcgcgcgccc
      541 tcgcctatcc tcaagggccg tgctctatta ggctgaacaa cgggttgcgg ttttgtcgag
      601 gcgaatttga gctcccaggc gaccgccgcg atcacgatgc gtctcgtcgg cctggcgacg
      661 cggtcctatg tcgagcgcct cgcgcggcct ttcggcgcgc tggcgcgccg catcgcgagc
      721 ctcggcgcgc ggcggccgga gcggctggcg attgcgccaa gggacatccg caccgccgat
      781 ccgatcgcgg cgcgcgacat ctatgccggg attttcgcct tggccggccg caccggcgtc
      841 gcgcatggcc gttcgccttt cgaaatcccg ccgccgtcga aaggctgggc cgaggccttg
      901 catggtttcg gctggctccg cgatctcgcc gccgccgaca cggctttgtc gcgcgccaat
      961 gcgcaggcgc ttgtcgagga ttggctcaat gtcggggccc gcaaaggctt cgccgtcgaa
     1021 tccgccatca tcaatgatcc gcgcgtcgcg gcgcgccggc tgatgtcgtg gctcagccat
     1081 tcgccggtca ttctcgaaaa cgccgatcgc gccttctacc gccgcttcgc gcgcagcctt
     1141 ggcgggcagg cggcgccgat cgagcgggcg ctcgccggcg gcctcaaggg cgaggcgcgg
     1201 ttggtggcgg cgatcgcgct gacccagacc ggcctgtcgc tcgaaaacgc ggggacgctg
     1261 ctccgccgcg ccggccggct gctcgcagcc gaactgagtc gccagattct cgccgatggc
     1321 ggccatgtca gccgcaatcc gcgcgtcctg gtcgacgcgc tgctcgatct cctgccgacg
     1381 cgccaggctt tcgccgcccg cggcctcgcc ccgcccgagc agctcctcaa cgccatcgac
     1441 cggatgacgc cgatcctgcg catgttccgc catgccgacg gcgcgctcgc caatttcaac
     1501 ggcatggggg tgagcgagcc ggagacgctc gcgaccttgc tcgcctatgg cgatcccggc
     1561 gcggcgatta tggatgcgcc ttattccggc tatcagcgcc tggaagccgg cggctcgatc
     1621 gctattatcg atgtcggcaa gccgccgcct tggcagtttg cgcaagacag ccatgccggc
     1681 tgcctctctt tcgaattctc ctccgacgcg catcggatta tcgtcaattg cggtgcgccg
     1741 ccgcccgata tgagcgaggc gcggctcgcc gcccgcgcca ccgccgccca ttcgaccctg
     1801 gtcgtcgccg atcattcgag ttgccgcttc gccgcgccga ccggactcga agcctttgcg
     1861 ctgggcgcgg cgctgcaagg gccttcgctc gtcgaggcga aacgcggcgc ggacgaagac
     1921 tggctgcgcg tcgacgccgt tcacgacggc tatgcgagag ctttcggcgt cgctcatcat
     1981 cgcatgctcg ccttggcgcg cgacggcgct cagctttgcg gcgaggaccg tctggcgccg
     2041 agcggcaagg cgcaagccgc cggcgagacg ccgatcgcgt cgcgctttca tctccacccc
     2101 gacattgcgg cggaatcgga gggcgaggcc gcggcggcgg tgctggtcac gccttccggc
     2161 caagcctggc gcttcgaggc aggcggccgc gacatcgcga tcgaggaaag cattttcttc
     2221 gccgcgcccg gccggccgcg ccgatcgagc cagttggtcg tccatgcgac gatcgccgaa
     2281 gccgcgccga tcgtctggtc gctgacgcgc gcctgaaagt acgagcgaag cggagcgaaa
     2341 cgccccgccg ccattgcggt cggcgaagcc ccctccagcc ttccggcctc gggaaaaaca
     2401 tgctacccgc cgctccggcg ggcccaatct ggccggccgt ataggattcc catggcgaat
     2461 gatcttgcgc gggtcggccg cgccctgatt tcggtttccg acaagaaggg gctcatcgcg
     2521 ctcgctggcg cgctcgccgc gcacggcgtt gaattggtct cgaccggcgg caccgccaag
     2581 gccctggccg acgccggcta cgccgtgcgc gacgcggccg agctcaccgg ctttccggaa
     2641 atgatggacg ggcgcgtcaa gacgctgcac cccggcatcc atggcggtct gctcgcggtg
     2701 cgcgaaaatc cggcgcatga agcggcgatg ctcgcgcaca acatcgcgcc gatcgatctg
     2761 ctcgtcgtca atctctatcc gttcgaagag acgctgcaac gcggccgctc gttcgacgag
     2821 atgatcgaga atatcgacat tggcggcccc gccatgatcc gcggcgcggc caaaaatcac
     2881 agcgacgtcg ccgtgatcgt cgagccgaac cagtacgcaa gcctgatcga agagctacag
     2941 cgccatgacg gcgcgaccag cctcgccttc cgccgcgcgc tcgcccagcg cgcctatgca
     3001 cgcactgccg cctacgacgc agcgatctcc aattggctcg ccggcgtgac cggcgaagcc
     3061 gcgccgcctt ggcgcgcgtt cggcggcgct ctgatcgagg cgatgcgcca tggcgaaaac
     3121 ccgcatcagg cggccggctt ctacgccggt cccgaaaagc gtccgggcgt cgccaccgcc
     3181 cggcaagcgc agggcaagca gctctcctac aacaatgtca acgacaccga cgcggctttc
     3241 gaactcgtcg ccgagttcga tccgacccgt gtcgccgcgg ttgcgatcat caagcacgcc
     3301 aatccgtgcg gcgtcgccga aggcgaaacg ctggtcgaag cctatcgcaa ggcgctggcc
     3361 tgcgactcgg tgtcggcctt cggcggcatt gtcgcgctca accgcacgct cgacgcggat
     3421 gcggcgcgcg caatcaccga catcttcacc gaagtgatca tcgcgccgga cgcgacggcg
     3481 gaagccatcg ccatcgtcgg cgccaagaag aacttgcggc tgctgctggc cggcggcctg
     3541 cccgatccgc gcgccgccgg gctttacgcc aag
//
LOCUS       DATGJW010000419         4971 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1119439, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000419 DATGJW010000000
VERSION     DATGJW010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4971)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4971)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4971
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1119439"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(84..698)
                     /locus_tag="VKU03_12560"
     CDS             complement(84..698)
                     /locus_tag="VKU03_12560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008137820.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1285 domain-containing protein"
                     /protein_id="HLJ72144.1"
                     /translation="MANGSSQSAERPARPPEQALQGLEAASAIRKRGPAPVHLWNPPY
                     CGEIDMRIAADGTWFYNGSPIGRLPLVQLFASILRRDDDSYVLVTPVERVGVKVDDAP
                     FLAVEMTVEGEAAARRITFRTSVEDFVTIDAEHPLRFEPGASEGLKPYALVRGGLWAL
                     VKRALFYDLVALGETRTHQGEDWFGIVSSEIFFPMTRAAEIEGL"
     gene            984..1994
                     /locus_tag="VKU03_12565"
     CDS             984..1994
                     /locus_tag="VKU03_12565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLJ72145.1"
                     /translation="MTVQDAAIANIEEAVARSAEAALEKIAAARAAIGKVIFGQDKVV
                     EQALVTLISGGHGLLIGVPGLAKTKLVETLGIVFGLDGRRVQFTPDLMPSDILGSEVM
                     EEASDHTRSFRFIKGPIFAQLLMADEINRASPRTQSALLQAMQEHHVTIAGARHDLPR
                     PFHVLATQNPLEQEGAYPLPEAQLDRFLMQIDVAYPDRVAERRILIETTGEAEAKAKP
                     AMSADDLMEAQRLARRVPVGESVVEAILDLVRAARPGEGDAEATKSISWGPGPRAAQA
                     LMLATRARALVDGRLSPSKDDIVALAEPVLKHRMALTFAARAEGETVSDLIGRLVAKT
                     IG"
     gene            2092..3039
                     /locus_tag="VKU03_12570"
     CDS             2092..3039
                     /locus_tag="VKU03_12570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HLJ72146.1"
                     /translation="MTVAAPTRVASASAANAGQARWRSEAATLAARLPSLVVAAKQIA
                     QSVMYGVHGRRRAGPGENFWQFRPFISGEPATGIDWRRSAREDRAYVREREWEAAHTV
                     WLWVDRSASMRFASPLAQASKIERGVVLGLAAADLLVRGGERVGLLGLTQTVASRGVI
                     ERFAESIAVDERLASEQAALPASQPLKAHAKAVLIGDFLSEEEMVRRSIATLSAQGAE
                     GHVLMIVDPIEETFPYQGHTEFIGADGATRLRAPRAQGLRQEYLKRLQRHREAVREAC
                     LSRGWSFAIHRTDRPASEALLALRMRLEAPPAYLSARGA"
     gene            3041..>4971
                     /locus_tag="VKU03_12575"
     CDS             3041..>4971
                     /locus_tag="VKU03_12575"
                     /inference="COORDINATES: protein motif:HMM:TIGR02226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA domain-containing protein"
                     /protein_id="HLJ72147.1"
                     /translation="MFGLPLAFTAPAALVSLVLLGALYVFLRITPPRPRQTIFPPLRL
                     LLGIDRKEQTPARTPWPLLALRLAIAAAAVIAMAGPMWNPSLFGGGSGPILMIVDDGW
                     PAAPSWEARKALLVARLEAAAHDNRLVALLPVSQGGVDIAPAEAARNAERLRALEPQP
                     YAPNHKDILPPIRKFLAQFPKAEIVWLADGLSIGETDSFAHELAGLANGHDASVLIDN
                     GTALGLSSVENLTGALQAKVLRAGATARQRGALHALDAKGLTIGEAPFDFAAADTATA
                     TFDLPIELRNEVSRITIDNERSAGAVVLLDERWRKRRVAIASGVSVDVAQPLLSPTYY
                     LSRAIGPFADLREWRDSASDPIVSLIGEKPSVVMLADMSVAGGPAHDALTKFVDEGGV
                     LVRFAGSRLAASADDLTPTELRRGGRTLGGALSWETPKKLAAFDRDSPFFGLTVPEEV
                     TVSRQVLAEPEPGLANKTWARLADGTPLVTADRRGKGVIVLFHVTADTTWSNLPLSGL
                     FVDMLRRIVALAAQSAPEAEKDAKGVVPSRPPKSVLDGYGALGAPPASAQPIAADFAG
                     PADAAHPPGFYGTGEALTAVNTLPTDEALKLADYAGLPVKTNAFAVAAPLDLRLWLLT
                     AAFIGLLIDVLASLWIGGAL"
BASE COUNT          788 a         1734 c         1597 g          852 t
ORIGIN      
        1 cgcgccttcc ggcagatcca gcgacagccg cgccttggcg cgggcgcgaa aatcctcggc
       61 ggagaagaaa ttgggcggcg cgttcacagg ccctcgatct ccgcggcgcg ggtcattggg
      121 aaaaaaatct cgctggagac gatgccgaac cagtcttcgc cttgatgcgt ccgtgtttcg
      181 ccgagcgcga ccaggtcgta gaacagcgcg cgcttgacga gagcccaaag cccgcctcgc
      241 accagcgcat aaggcttcag gccctcggag gcgccaggct caaaacgcag cggatgttcg
      301 gcgtcgatgg tgacgaaatc ctcgacgctc gtccggaaag tgattcgccg cgcggccgcc
      361 tccccctcga ccgtcatttc cacagcgaga aagggcgcat cgtccacttt gacgccgacg
      421 cgctcgaccg gcgtgacgag cacgtaggaa tcgtcatcgc gtctgaggat cgaggcgaag
      481 agctggacca gcggcagacg gccgatcggc gagccgttgt agaaccaagt cccgtcggcg
      541 gcgatccgca tgtcgatctc gccgcaatag ggcggattcc acaaatggac cggcgccggc
      601 ccgcgctttc gaatggcgga ggccgcctcg agcccctgca aggcctgctc tggcgggcgc
      661 gccggccgtt cagccgattg tgaggagccg ttcgccataa tctcgcgcat ctttgtgggc
      721 aagcatcttg cttggccccg cggcgttcga cttcgccgct ccagacctta atgtaagcgg
      781 gtttccttgg ttgaccaatg taaggcggct tcccctcgcc caaccctctc ccgccagtgg
      841 gagaggcggc gcgctgagcg cttcgtcagc gccctagaga gtaagatgag acggcggcgg
      901 caatcccttc tcccgcttgc ggcagaaggg agcggcgaag ccgccggaga gcgtaaccgc
      961 agcctttgct tttcggagaa gacatgaccg ttcaagacgc cgcaatcgcc aatatcgagg
     1021 aggccgtcgc gcgctccgcg gaggcggcgc tggagaaaat cgccgccgcc cgcgccgcga
     1081 tcggcaaagt cattttcggc caggacaaag tcgtcgagca ggcgctggtg acgcttattt
     1141 ccggcggtca cggtttattg atcggcgtgc ccggcctcgc caagacgaaa ctggtcgaaa
     1201 cgctcggcat cgtcttcggc ctcgacgggc gccgcgtgca attcacgccg gacctgatgc
     1261 cctccgacat tctcggctcg gaagtgatgg aggaggcgag cgaccacacc cgctccttcc
     1321 gcttcatcaa gggtccgatc ttcgcgcaat tgctgatggc cgatgagatc aaccgcgcca
     1381 gcccgcgcac tcagtctgcg ctgctccagg cgatgcagga gcatcacgtc acgatcgccg
     1441 gcgcgcggca tgatctgccc cgtccgttcc atgtgctggc gacgcaaaac ccgctcgagc
     1501 aggagggcgc ctatccgctg cccgaggcgc agctcgatcg gtttctcatg cagatcgacg
     1561 tcgcctatcc cgaccgcgtc gccgagcggc gcattctcat cgagacgaca ggcgaggccg
     1621 aagccaaggc caagcccgcg atgagcgcgg acgatctcat ggaggcgcag cggctcgccc
     1681 gccgtgtgcc ggtcggcgaa agcgtcgtcg aggcgatcct cgatctcgtc cgcgccgcgc
     1741 ggccgggcga gggcgacgcc gaggcgacca aatccatctc ctggggcccc ggcccgcgcg
     1801 ccgcacaagc tctcatgctg gcgacccgcg cccgcgccct tgtcgacggc cgcctgtcgc
     1861 cctccaagga cgatattgtc gcgctggccg agccggtcct gaaacaccgc atggcgctga
     1921 ctttcgccgc gcgggcggaa ggcgaaaccg tgagcgatct catcggccgc ctcgtcgcca
     1981 agacgatcgg gtaagtaggc tccctttgaa agatcaactg cggctacccc tcaccccaat
     2041 cctctcccgc aagcgggaga gggggcgcga agcgccggat aaggggtagc cgtgaccgtc
     2101 gccgccccta cccgcgtcgc ctcggcctcg gccgccaatg ccggccaggc gcgctggcgc
     2161 agcgaagcgg cgactctcgc cgcgcggctg ccgagcctcg tcgtcgcggc caagcagatc
     2221 gcgcaaagcg tgatgtatgg cgtgcatggc cgacgccgcg ccgggcccgg cgagaatttc
     2281 tggcaattcc ggcccttcat ttccggcgag ccggcgacgg ggatcgattg gcgacgctcg
     2341 gcgcgggagg atcgcgccta tgttcgcgag cgcgaatggg aagcggcgca taccgtctgg
     2401 ctatgggtcg accgctccgc ctcgatgcgc tttgcttcgc cattggcgca agcgtcgaaa
     2461 atcgagcgcg gcgtcgtact cggcctcgcc gcggcggatt tgctcgtgcg cggcggtgaa
     2521 cgtgtcggcc ttctcggcct gactcagact gtcgcgtcgc gcggcgtcat cgagcgcttc
     2581 gccgagtcga tcgccgtcga cgagcgcctc gcctcggagc aggcggcgct gccggcctcg
     2641 caacccttga aggcccatgc caaggccgtg ttgatcggcg atttcttgag cgaagaggag
     2701 atggtgcggc gctcgatcgc gacgctttcg gcgcaaggcg ccgaagggca tgtgctgatg
     2761 atcgtcgatc cgatcgagga gactttcccc tatcaaggcc acaccgaatt cattggcgcc
     2821 gatggcgcga cgcgcctgcg cgcgccgcgc gcgcaaggcc tgcggcagga atatctgaag
     2881 cgcctgcaac gccatcgcga ggctgtgcgc gaggcctgtc tgagtcgtgg ctggagcttc
     2941 gccatccatc gtaccgaccg gccggcttcc gaagctttgc tcgctttgcg gatgcggctc
     3001 gaggcgccgc ccgcttacct ttccgcacga ggcgcctgac atgttcggcc tgcctctcgc
     3061 cttcaccgct ccggccgcgc ttgtctcgct ggtcctgctc ggcgcgcttt atgtcttctt
     3121 gcgcatcacg ccgccgcggc cgcgtcagac gatcttcccg ccgctgcgcc ttctgctcgg
     3181 catcgaccgc aaggagcaga cgcccgcccg cacgccctgg ccgcttctgg cgttgcgtct
     3241 cgccatcgcc gccgccgccg tgatcgccat ggccggcccg atgtggaatc cctcgctctt
     3301 tggcggcggc tccgggccga tcctgatgat cgtcgacgat ggctggccgg cggcgccgag
     3361 ttgggaagcg cgcaaggcgc tcctcgtggc gcggcttgaa gcggcggcgc acgacaaccg
     3421 cctcgtcgct ttgcttcccg tctcgcaagg cggcgtcgat atcgcgcccg ccgaggccgc
     3481 gcgtaacgcc gagcgcttgc gcgcgctcga gccgcaaccc tatgcgccga accataagga
     3541 cattcttccg ccgatccgga aattcctcgc ccagtttcca aaggcggaaa tcgtctggct
     3601 ggcggacggc ctgtcgatcg gcgaaacgga ttcgttcgcg cacgaacttg ccggtctcgc
     3661 caatggccac gacgccagcg tgctcatcga caatggaacg gcgctcggtc tgtcgagcgt
     3721 cgaaaatctt accggcgcgc tgcaagccaa ggtgcttcgc gccggcgcca cagcgcggca
     3781 gaggggcgcg ctgcacgcac tggacgccaa gggcctgacg atcggcgaag cgccgttcga
     3841 tttcgccgcc gccgatacgg cgaccgcgac tttcgatctg ccgattgaat tgcggaacga
     3901 agtctcgcgc ataacgatcg acaatgagcg ttccgccggc gccgtcgtcc ttctcgacga
     3961 acgctggcgc aagcggcgcg tcgccatcgc ctccggcgtc agcgtcgacg tcgcccagcc
     4021 gctcctttcg ccgacctact atctatcccg cgcgatcgga cctttcgccg acttgcggga
     4081 gtggcgtgac agcgccagcg atccgatcgt ttcactgatc ggcgaaaagc ccagtgtcgt
     4141 gatgctcgcc gatatgagcg tcgccggcgg cccggcgcat gacgctttga ccaaattcgt
     4201 cgacgagggc ggcgtgctcg tacgtttcgc cggcagccgg ctcgccgctt cggcggacga
     4261 tctgacgccg accgagctcc gccgcggcgg ccgcaccctc ggcggcgcgc tctcttggga
     4321 gacgccgaaa aagcttgcgg ctttcgatcg cgacagcccc ttcttcggtc tgaccgtccc
     4381 cgaggaagtc accgtctcgc gccaggttct cgccgagccc gaacctggtc tggccaacaa
     4441 gacctgggcg cgtctcgccg acggcacgcc tttggtgacc gccgatcggc gcggcaaagg
     4501 cgtcatcgta ctcttccatg tcacggccga tacgacctgg tcgaacctgc cgctctccgg
     4561 ccttttcgtc gacatgctgc gccgtatcgt tgcgctcgcc gcgcaatcgg cgccggaggc
     4621 ggaaaaagac gccaaaggcg tcgtcccctc ccgtccgccg aaaagcgttc tcgacggtta
     4681 tggcgcgctt ggcgcgccgc cagcctcggc gcaaccgatc gccgccgatt tcgccggccc
     4741 ggccgacgcc gctcatccgc ctggcttcta tggaaccggc gaagcgctga ccgccgtcaa
     4801 tacgctaccg accgatgaag cgctgaagct cgccgactat gccggtttgc ccgtgaagac
     4861 caacgccttc gccgtggcgg cgccgctcga tctgcgcctt tggctgctga ccgcggcttt
     4921 catcggtctc ttgatcgatg ttctggcaag cctttggatc ggcggcgcgc t
//
LOCUS       DATGJW010000420         8697 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_491297, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000420 DATGJW010000000
VERSION     DATGJW010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8697)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8697)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8697
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_491297"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..1202
                     /locus_tag="VKU03_12580"
     CDS             24..1202
                     /locus_tag="VKU03_12580"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018240716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72148.1"
                     /translation="MRAYARGNIARLTVAQALAGANSVVAYATGAIVGSTLAPNPALA
                     TLPISIFVIGMAASTLPAGVIAQQYGRRAAFLVGTGCGVLVGLLAALAVALGSFGLFC
                     MAMIFGGAYAAVVLTFRFAAADCVSPELRPRALSMVMAGGIFAGVIGPQLVTYTMYLW
                     MPHVFAATFLAQACVAALSALVLLGVRLPAPTAAELADGRPLGVIARQPRFAIAVICG
                     VVSYLLMNFLMTAAPLAMHLCGLSQEASNLGIQWHVIAMYAPGFFTGRLIARFGAPSV
                     VVAGLALIGAAAAVGLLGVDVGHFWLTLILLGIGWNFGFVGASALVLECHRPEEKTRV
                     QSLNDFAVFGTMAVGSFLSGGLLAAYGWNTVLSLSFAPLALAIFALTGLAARSRGVAG
                     "
     gene            1437..2156
                     /locus_tag="VKU03_12585"
     CDS             1437..2156
                     /locus_tag="VKU03_12585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019400885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HLJ72149.1"
                     /translation="MDHPVVSREEWLEARKALLLKEKEATRLRDAVNAQRLALPWVKV
                     GKPYRFATPSGDESLAELFAGRSQLIVYHFMFGPEWQAGCPGCSFLADHLDGALPHLE
                     HHDVTLTAVSRAPLAKIEAYKRRMGWRFPWVSSFGGDFNADYGVSFDESDLAKGEVFY
                     NFRSIKSAEANDELPGLSAFAKDEDGQVYHTYSSYARGPEELIGALMILDRAPKGRNE
                     QRTMDFVRRHDEYEKNPAAHA"
     gene            2207..2980
                     /locus_tag="VKU03_12590"
     CDS             2207..2980
                     /locus_tag="VKU03_12590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384612.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ72150.1"
                     /translation="MSDNHGKFVWYELMTTDMAAAEPFYRSVVGWSARREDTPATPYT
                     IFSAGAAGIGGLMPLPKSARDMGVPPHWTGYISVDDVDVYARKVAEAGGAILVPPADI
                     PNVGRFAVAADPDGVDFNLFKPRDGAAPPAVAPGTVGHAGWRELYAGNLDAAFAFYSK
                     LFGWTKGEAFDMGAMGVYQLFATGAEPVGGMMTRPPTMPRPAWTYYFNVAGINDAAGR
                     VKAGGGQVVNGPMQVPGGSWIVQCLDPQGAMFALVSPQA"
     gene            3141..4022
                     /locus_tag="VKU03_12595"
     CDS             3141..4022
                     /locus_tag="VKU03_12595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YihY/virulence factor BrkB family protein"
                     /protein_id="HLJ72151.1"
                     /translation="MRRIGKIFGIFLDAYWRFLAQDGWAIASYMGLSALTSMFPFLIF
                     VTALAGFFGTDNLAEEAARLIFAVWPKEVAGPIAGEVQSVLTAPRGGLLTIGAALALY
                     FASGAIEAARVGLNRAYGSVETRPWWLLRLESIAFVVVAAFGLLALAFLVVLGPLIWS
                     TTLRYLPQLAPLGFLVTFIRLGAAVVLMAITLFLAHILLPRGRRRLIDLAPGVALTFT
                     ACIAFGEVFGAYLSEYARSYVSTYAGLASVMIALVFLYAMSGIFIYGGELNAAIMRAR
                     EATKVSGGVSPKGEGQG"
     gene            complement(4217..5500)
                     /gene="ccrA"
                     /locus_tag="VKU03_12600"
     CDS             complement(4217..5500)
                     /gene="ccrA"
                     /locus_tag="VKU03_12600"
                     /EC_number="1.3.1.85"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921939.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043880 - crotonyl-CoA reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crotonyl-CoA carboxylase/reductase"
                     /protein_id="HLJ72152.1"
                     /translation="MREAATIQSEVGELKDLYDIGEIPPLGHVPAKMRAWVVRRERHG
                     PPEQSMQIEVIATWPLDSHDVLVLVMAAGVNYNGVWAALGQPISPFDVHKQPFHIAGS
                     DASGIVWAIGAKVTRWKVGDEVVVHCNQDDGDDEECNGGDPMYSASQRIWGYETPDGS
                     FAQFCRVQDRQLMPRPKHLTWEESASYTLTLATAYRMLFGHEPHRLKPGDNVLVWGAS
                     GGLGVFGVQLCAAAGANAIGVISDEEKRDYVMSLGAKGVLNRKAFKCWGQLPKVNTDD
                     YAAYMAEARKFGKAIWEITGKKDVDIVFEHPGEQTFPVSCFVVKRGGMVVFCAGTTGY
                     NFTFDARFVWMRQKRIQGSHFAHLKQAAAANKFVIERRIDPCLSEVFPWDKIPAAHTK
                     MWRNEHKPGNMAVLVNAPTTGLRTLDDVIEAGRKA"
     gene            5806..6156
                     /locus_tag="VKU03_12605"
     CDS             5806..6156
                     /locus_tag="VKU03_12605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009337745.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2282 domain-containing protein"
                     /protein_id="HLJ72153.1"
                     /translation="MSQRILSALVASSFAAALGSIPLSSAGAEDAMMKPMSKSEMMKM
                     RAMTAKEVKSGKMEKCYGVALKGQNDCYAGPGTTCAGTSTVDYQGNAFKLEPKGSCTA
                     MQTPKGPGSLTPKA"
     gene            6223..7113
                     /locus_tag="VKU03_12610"
     CDS             6223..7113
                     /locus_tag="VKU03_12610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 domain-containing protein"
                     /protein_id="HLJ72154.1"
                     /translation="MPPALSSTLPRAIPPRAGVGLKPQHYSTIIETKPDIGFFEAHAE
                     NYMCAGGPPHRYLGAIREHYPLSLHGVGLSIGADRPLNRDHLARLKSLNERYAPGLFS
                     EHLAWSTHDGAFLNDLLPVPYTEETLARVCEHIDEVQDALGRQMLLENPSTYIAFAES
                     DYAETGFIAEVARRTGCGLLLDVNNVYVASTNQEWDPIAYIEAYPLASVQEIHLAGYA
                     READEKGRSLLIDTHDRPVDEIVWDLFARVVARIGPTPTLIEWDAAVPAWPTLKAEAE
                     RAEAIMLAGEREGPRRAASC"
     gene            7097..7714
                     /locus_tag="VKU03_12615"
     CDS             7097..7714
                     /locus_tag="VKU03_12615"
                     /inference="COORDINATES: protein motif:HMM:NF021364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-binding domain-containing protein"
                     /protein_id="HLJ72155.1"
                     /translation="MRRLAERQRDFAAALLDAGRPLPKGIVGPDGEPSAKRFAVYRNN
                     VIVGLIEALGDAFPAVRRIVGEDFFREMARAYVVREPPSSPILLDYGDKFADFVDGFA
                     PAAGLPYLRDVARIERAWREAYHASEAEPLDAAAFAAIGPDRYPELRLLFHPSARVVR
                     SSFPAVTIWRMNVADGVPAPVDLAAGGEDAFVVRPRAVVEARLIP"
     gene            7885..8406
                     /locus_tag="VKU03_12620"
     CDS             7885..8406
                     /locus_tag="VKU03_12620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018329927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLJ72156.1"
                     /translation="MTAPHAPLRLADVEKLLRAITGALRRVAIIVAPPVLRIALAVPF
                     FRSGLTRWDGFLSLSPSAAYLFEEEFKLHIFGQIYDFPAPIVVAHIVGAAEIVLPVLL
                     IAGFATRFAALALLIMTGVIQLVAPDGWANFHLPWAAMALAIIALGPGLLSLDFLIDR
                     LRRFREMGGRRSH"
     gene            8506..>8697
                     /locus_tag="VKU03_12625"
     CDS             8506..>8697
                     /locus_tag="VKU03_12625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72157.1"
                     /translation="MDQVKYRIYDPRLAPRRTKLEIPGWAGTRQPRADGSREQVWHCV
                     PFSEGAQYGIELFYPYDNEL"
BASE COUNT         1355 a         2840 c         2799 g         1703 t
ORIGIN      
        1 tgaccgcgca gtcgtcctcg tccattcgcg cctacgcgcg gggcaacatc gcaagattga
       61 ccgtcgcgca ggcgctcgcc ggggcgaact cggtggtcgc ctatgcgaca ggcgcgatcg
      121 tcggaagcac gctcgcgccc aacccggcgc tcgcgacatt gccgatctcg attttcgtga
      181 tcggcatggc ggccagcact ctgccggccg gcgtcatagc ccagcaatat ggtcgccgcg
      241 ccgctttcct ggttggaaca ggatgcggcg tgcttgtcgg cctgctcgcg gccttggcgg
      301 tggcgctggg ctccttcggg ttgttttgca tggcgatgat tttcggcggc gcttatgccg
      361 cggtggtcct gaccttccgc ttcgccgccg ccgattgcgt gtcgccggag cttcgcccgc
      421 gggccttgtc catggtcatg gcgggcggaa tcttcgccgg ggtgatcggg ccgcaactcg
      481 tcacctatac gatgtatcta tggatgcccc atgtgtttgc ggcgaccttc ctggcgcaag
      541 catgcgtcgc cgcattgtcg gcgctcgtcc tgcttggcgt tcgtcttccc gcgccgacgg
      601 cggcggagct cgccgacggg cgcccgctgg gggtcatcgc gcgccagcct cgcttcgcca
      661 tcgcggtcat ctgcggggtc gtctcctatc tgctgatgaa ctttctgatg accgccgcgc
      721 ccctcgccat gcatctatgc ggcctgtcgc aggaggcgtc caacctcggc atacagtggc
      781 atgtgatcgc gatgtacgcc ccaggcttct tcaccgggcg gctgattgcg cgctttggcg
      841 cgcccagcgt cgtcgtcgcc ggcctcgccc tgatcggcgc cgccgcagcg gtcgggttgc
      901 tcggcgtcga tgtcgggcat ttctggctca cgctcatcct gctcggcatt ggctggaact
      961 tcggcttcgt cggcgcatcg gcgcttgtgc tcgagtgtca ccgtcccgag gaaaagacgc
     1021 gcgttcagtc gctcaacgac ttcgccgtgt tcgggacgat ggccgtcggc tccttcctgt
     1081 cgggcggctt gctggcggcc tatggctgga acaccgtgct gtcgttgtcg tttgcgccgc
     1141 tcgcgctggc gattttcgcc ttgaccgggt tggcggcgcg cagccgcggc gttgccgggt
     1201 gattgaaagc ttctcgccgg atgcgcgctg agcggcagtc ggcagtcggc aatcggcaga
     1261 tcggggcggc gggagcggat cggcgaggcc atgcgcaacg cgcgacgggt tgaaaaaaaa
     1321 gcgtggcgcg ttgtcgattt caggccgcct caaacgtctt cagggtggtg ctgcgcgagc
     1381 gtccggccgt cggcgacgcg agcgcgggcg aaagcgccca tcggaggagc gtcatgattg
     1441 atcacccggt cgtctcgcga gaggaatggc tcgaagcgcg gaaggcgctg cttctaaagg
     1501 agaaagaggc gacgcggctg cgcgacgcgg tcaacgcgca gcgcctggcg ctgccctggg
     1561 tcaaggtcgg gaagccttac cgcttcgcga cgccttcggg cgacgaaagc ttggccgagc
     1621 tctttgccgg acgcagccag ctcatcgtct atcatttcat gttcggcccc gaatggcagg
     1681 ccggctgtcc cggctgctcg ttcctcgccg atcatctcga cggcgctctg ccgcatctcg
     1741 aacatcatga tgtcacgctg acggcggttt cgcgcgcgcc gctcgccaaa atcgaggcct
     1801 ataaaagacg gatgggctgg cgtttcccgt gggtctcgtc tttcggaggc gattttaacg
     1861 ccgattacgg cgtctcgttc gacgagagcg atctcgccaa gggcgaggtc ttctataatt
     1921 tccgctccat caaatcggcc gaggccaatg acgagctacc gggcctcagc gccttcgcca
     1981 aggacgagga cggtcaggtc tatcacacct attcgagcta tgcgcgcggc ccggaggaac
     2041 tgatcggcgc gctgatgatt ctcgatcgcg cgcccaaggg ccgcaacgag caaaggacga
     2101 tggacttcgt gcgccgccac gacgaatatg agaagaaccc cgccgctcat gcctgatgcg
     2161 cgcctgagcg gctttgccct accgctttca tgaaaggagg ctcgcgatga gcgataatca
     2221 tgggaaattc gtctggtacg aattgatgac caccgatatg gcggcggccg agcccttcta
     2281 tcgcagcgtc gtcggatgga gcgcccggcg cgaggatacg ccggccacgc cctatacgat
     2341 cttcagcgcc ggcgcggccg gaatcggcgg cttgatgccg ctgccgaaat cggcgcgcga
     2401 tatgggcgtc ccgccgcatt ggaccggcta tattagcgtc gacgacgtcg acgtttacgc
     2461 aaggaaggtc gccgaagcgg gcggcgcgat tttggtgccg cccgccgata ttccgaatgt
     2521 cggccgcttc gcggtcgccg ccgatccgga tggcgtcgat ttcaacttgt tcaagccgcg
     2581 cgacggcgcg gccccgccgg ccgtcgcccc agggactgtc ggccatgccg gctggcggga
     2641 gctctacgcc ggcaatctcg acgcggcctt cgcgttttat tcgaagctgt tcggctggac
     2701 caagggcgag gctttcgata tgggcgcgat gggcgtctac cagctcttcg cgaccggcgc
     2761 cgagccggtc ggcggcatga tgaccaggcc gccgactatg ccgcggcccg cctggacgta
     2821 ctatttcaac gtcgccggaa tcaatgatgc ggccggccgg gtgaaagccg gcggcggcca
     2881 agtggtcaac ggcccgatgc aggtccctgg cggctcctgg atcgtccaat gcctcgatcc
     2941 gcagggggcc atgttcgcgc tggtcagccc gcaagcctga ccgggggcgc cccacacgga
     3001 aagtacgcga gggcgagcgc cggcctgccc ggcttgcgcc gcctgcgccg gacgggcgcg
     3061 ccgcgcggca ttgacagcca gcggcgatcg gccaaaaacg aaagccggag cggccggaca
     3121 atcccgctcc ggctttcgcg atgcggcgga tcggcaaaat cttcggcatt ttcctcgacg
     3181 cctattggcg ttttctcgcc caagacggtt gggcgatcgc gagctatatg ggcttgtcgg
     3241 cgctcacctc gatgtttccc tttcttatct tcgtcaccgc cctcgccgga tttttcggca
     3301 cggacaatct cgccgaggag gcggcgcggc tgattttcgc cgtctggccg aaggaggtcg
     3361 ccggtccgat cgccggcgag gtgcagagcg tcttgaccgc gccgcgcggc ggcttgctga
     3421 cgatcggcgc ggctttagcg ctctatttcg cctcgggcgc gatcgaggcg gcgcgggtcg
     3481 ggctcaaccg cgcttatggc agcgtcgaga cgcggccgtg gtggctgttg cgcctcgaat
     3541 cgattgcctt cgttgtcgtg gcggcgttcg gcctgctggc gctcgctttc ctcgtcgtgc
     3601 tcggcccgct tatctggtcg acgactttgc gctacttgcc gcaacttgcg ccgctcggtt
     3661 ttctcgtcac cttcatccgg ctcggcgcgg ccgtcgtgct catggcgatc accttgttcc
     3721 tggcgcatat ccttttgccg cgcggccgcc ggcggctgat cgatctcgcg cccggcgtcg
     3781 ccttgacctt caccgcctgc atcgcgttcg gcgaagtgtt cggcgcctat ctttcggaat
     3841 atgcccgcag ctacgtctcg acctatgccg gcttggcgtc ggtcatgatc gccttggtct
     3901 tcctctacgc catgtccggc atcttcatct atggcggcga gctcaatgcg gcgatcatgc
     3961 gcgcccgaga ggcgacgaag gtaagcggcg gcgtgtcccc gaaaggcgag gggcaggggt
     4021 gaggagtaca gcgctccaaa gtacgatcgc tcctcgcttt cctcccccgt cgaagacggg
     4081 ggagggggac cgcgcgtaag cgcggtggag ggggcctcgg tctctcagga taatcccatc
     4141 cgcccacgac cccctccacc ggccttcggt cggtccccct cccccgttcc gctgcgcttc
     4201 acgggggagg attcgtctac gccttccttc ccgcctcgat cacgtcgtcg agcgtacgaa
     4261 ggccggtcgt cggcgcgttg accagcacgg ccatgttgcc cggcttatgc tcgttccgcc
     4321 acatcttggt gtgggcggcg ggaatcttgt cccagggaaa gacttccgac aggcacggat
     4381 cgatccgccg ttcaatgacg aacttgttgg ccgccgccgc ttgcttcaga tgggcgaaat
     4441 gcgagccttg gatgcgcttc tgccgcatcc agacgaagcg ggcgtcgaag gtgaaattgt
     4501 agccggtcgt gccggcgcag aagaccacca tgccgccgcg cttgacgacg aagcaggaga
     4561 ccggaaacgt ctgctcgccg ggatgctcga aaacgatgtc gacgtccttc ttgcccgtga
     4621 tctcccagat cgctttgccg aatttgcgcg cctcggccat ataggcggcg tagtcgtcgg
     4681 tattgacctt gggcaattgc ccccagcact tgaaggcctt gcggttgagc acgcctttgg
     4741 cgccgagcga cattacataa tcgcgctttt cttcgtccga aatgacgccg atcgcattgg
     4801 cgccggccgc cgcgcaaagt tgcacgccga acacgccgag gccgcccgaa gcgccccaca
     4861 caaggacgtt gtcgcccggc ttcaggcgat gcggctcgtg gccgaacagc atacggtacg
     4921 cggtggcgag ggtcagcgta taggacgcgc tctcctccca ggttagatgt ttgggccgcg
     4981 gcatgagctg gcggtcctgc acgcggcaga attgcgcgaa cgagccgtcc ggcgtctcat
     5041 agccccaaat gcgctgcgag gcggagtaca tcgggtcgcc gccattgcac tcctcgtcgt
     5101 cgccgtcgtc ctgattgcaa tggacgacca cctcgtcgcc gactttccag cgcgtcactt
     5161 tggcgccgat cgcccagaca atgccggatg cgtcggagcc ggcaatgtga aacggctgct
     5221 tgtgcacgtc gaacggcgag atcggctggc cgagcgccgc ccagacgccg ttgtaattga
     5281 ccccggccgc catcacgaga accagcacgt cgtggctgtc gagcggccag gtcgcgatga
     5341 cctcgatctg catggattgt tcgggcggcc catgcctttc gcgccgcacc acccaggcgc
     5401 gcatcttggc cggcacatgg ccgagcggcg ggatttcgcc gatgtcgtaa aggtctttga
     5461 gttcgccgac ttccgactgg atcgtcgctg cttccctcat cggggcgctc ccatcgcttg
     5521 cgccgcgcag cgggacgccg gctgcttttt gcgctgcagc atattggcgc cgcaaccgcg
     5581 cgggcgcaat attctttgat gcggagcaaa aagaacggcg accggtcgta aatctcaacg
     5641 ccggcgttgc ggatgacacc atgggagccg gctgggcgcg ccatccagcg tcgtggcgat
     5701 gtaacgtttc ttctgttcgc gccgaatgat gggcaggcgc tcacgataga ccgctcggac
     5761 gggcggcggc gcgccttcgg caaggaaacc cattcggagg agccaatgtc tcaacgcatt
     5821 ctatctgctc tcgtcgccag ctctttcgcg gcggcgctcg gctcgatccc tctgtcttcc
     5881 gccggcgcgg aagacgcgat gatgaagccc atgagcaagt cggaaatgat gaaaatgcgg
     5941 gcaatgaccg ccaaagaggt caagtcgggc aagatggaga aatgctacgg cgtcgcgctc
     6001 aaaggccaga acgattgcta tgcgggacct gggacgacct gcgcggggac cagcacggtc
     6061 gattaccagg gcaacgcctt caagctcgag cccaagggct cctgcaccgc gatgcagacg
     6121 ccgaagggac ctggaagcct gacgcccaag gcctgagacc gagccgattc cgggcggacg
     6181 cggcggcgcg cgtccgcccg cgtcgtcgtc cgggagtccg ccatgccgcc cgccttatcg
     6241 tcaaccctcc ctcgcgccat tccgccgcgc gccggagtcg gcctcaagcc ccagcattac
     6301 tcgacgatta tcgagacgaa gccggacatc ggcttcttcg aagcgcatgc ggaaaattat
     6361 atgtgcgccg gcggcccgcc acatcgttat cttggagcga tcagggagca ttatccgctg
     6421 tcgctccacg gcgtcggcct ctcgataggc gcggatcgcc cgctcaatcg cgaccatctc
     6481 gcaaggctca agtcgctgaa tgagcgctac gcgcccggac tcttttcaga gcatctcgcc
     6541 tggtcgacgc atgacggcgc gtttctcaac gatcttttgc cggttcccta caccgaggag
     6601 acgcttgccc gcgtttgcga gcatatcgat gaagtccagg acgcgctcgg ccggcaaatg
     6661 ctgctcgaaa atccctcgac ctatatcgct ttcgcagaaa gcgattacgc tgaaaccggc
     6721 ttcattgcgg aagtcgcgcg tcgcaccggc tgcggcctcc tcctcgacgt caacaacgtt
     6781 tatgtcgcct cgaccaatca agaatgggac ccgatcgcct atatcgaggc ctatccgctc
     6841 gcctctgttc aggagattca tctcgccggc tacgcgcgcg aggcggatga gaaagggcgg
     6901 tcccttctca tcgacacgca tgatcgtccc gtcgacgaaa tcgtctggga tctcttcgcc
     6961 cgcgtcgtcg cgcgtatcgg gccgacgccg actttgatcg aatgggacgc cgcggtcccc
     7021 gcctggccga ccctcaaagc ggaggcggag cgagcggagg cgatcatgct tgccggcgag
     7081 agagaggggc cgcgccgtgc ggcgtcttgc tgaaaggcag cgggatttcg ccgccgcgct
     7141 gctcgacgcc ggccggccgc ttccgaaggg aatcgtcggc cccgacggcg agccgagcgc
     7201 caagcgattc gcggtctacc gcaacaatgt catcgtcggc ctgatcgaag cgctgggaga
     7261 cgcctttccg gccgtgcgcc gtatcgttgg ggaagatttc ttccgggaga tggcccgcgc
     7321 ctatgtcgtc cgcgagccgc ccagttcgcc gattctcctc gattacggcg acaaattcgc
     7381 cgatttcgtt gacggcttcg cgcccgcggc cgggctgccc tatcttcgcg acgtggcgcg
     7441 catcgagcgc gcgtggcggg aagcctatca cgcgagcgaa gccgagccgc tcgacgccgc
     7501 cgcttttgcg gcgatcgggc ctgatcgcta tcccgagctt cgcctcctgt ttcacccctc
     7561 agcgcgcgtc gtccgctcgt cctttccggc cgtcacgatt tggcgcatga atgtcgccga
     7621 cggcgtccct gcgccggtcg atctcgccgc cggcggcgaa gacgcttttg tcgtccggcc
     7681 tcgggccgtg gtcgaggcgc gcttgatccc ctagggcggc gcggaattca tcgaggcgct
     7741 cgccgaggga aagtccgtcc tcgctgcgac agcggccgcg atagaagcga agagacattt
     7801 cgatcttgcc gccaatctcg ccgggctcat cggcgccggc gcgttcgtcg attaccgcct
     7861 tggacaagag acgaggcagg gctaatgacc gcgccccacg cgcccctgcg ccttgctgac
     7921 gtcgaaaagt tgctgcgcgc cataacaggc gcgctccgta gagtggccat catcgtcgcg
     7981 ccgcccgtcc tgcggatcgc gcttgccgtc ccgttttttc gatccggtct cacccgatgg
     8041 gacggcttcc tctcattgtc gccgagcgcg gcgtatcttt tcgaagagga gttcaaactc
     8101 catattttcg ggcagattta tgattttccg gcgccgatcg ttgtcgctca tattgtcggc
     8161 gccgccgaaa tcgtgttacc ggttttgctc atcgccggct tcgcgacgcg cttcgcggcg
     8221 cttgccttgc tgattatgac aggcgtgatt cagctcgtcg cgccggacgg ctgggcgaat
     8281 ttccatctgc cttgggcggc gatggccctg gcgatcattg cgctcggtcc aggccttttg
     8341 tcgttggatt tcttgatcga tcgattacgg cgttttcgcg aaatgggcgg gcgccgttcg
     8401 cattgactga gagaatttgg gtccgcctca aggcccctgg gaaccccgac aggcgcaatt
     8461 gcgttgtgag tgacggaaaa atttagccgc gccggcgggg ggcctatgga ccaggtcaaa
     8521 tatcgcattt acgatccgcg cctcgcgccg cgccgcacca agctggaaat tccgggatgg
     8581 gcaggcacac gccagccccg cgccgacggc tcgcgcgaac aggtctggca ctgcgtgccg
     8641 ttttcggaag gggcgcagta cggcatcgag ctgttttatc cctacgacaa tgaactg
//
LOCUS       DATGJW010000421         6250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1326220, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000421 DATGJW010000000
VERSION     DATGJW010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6250
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1326220"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..748)
                     /locus_tag="VKU03_12630"
     CDS             complement(<1..748)
                     /locus_tag="VKU03_12630"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-binding oxidoreductase"
                     /protein_id="HLJ72158.1"
                     /translation="MRSQARAVVIGGGVVGVSTLYHLVKKGWTDSVLVERKELTSGST
                     WHAAGLLPLFNVSYSVGQIHKYSVALYKRLAAETGMNVGFAQVSNIRLARTKDRLDEY
                     RYYAGVAATIGVEVKFLTPGEVKEIWPLCEIDGIVGAIQHPEDGYIQPADLTQALARG
                     ARNGGGEINRNTVVTAIERTASGEWKVVTDKGEIVCEHVVAATGNFARQTGRMVGINV
                     PVIPVQHQYIVTEKHPAIMARKAQGLPEMGV"
     gene            complement(892..2448)
                     /locus_tag="VKU03_12635"
     CDS             complement(892..2448)
                     /locus_tag="VKU03_12635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019858696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HLJ72159.1"
                     /translation="MSVEPSATGAPAHEARRGREARRAARAQRAVAHVPYITRNIPLT
                     EILSEEGLALIERNAETLLQEVGIEFREFPRALALFKEAGCDIRGERVRFPRGLARKL
                     CATTPASYIQCARNRGRDVAIGGNHTVFAPNYGSPFVHDLDKGRRYGTIEDFRNFVKL
                     SYATPFIHHSGGTVCEPVDLPVNKRHFHMVYAHMKYSDKPFMGSVTKPERAQDTVTMC
                     EILFGADFVQSNTVCTSLINANSPLVWDSSMLGAAEVYARNNQACIITPFILSGAMSP
                     VTVAGTLTQVLAEVMAGTAFCQLTRPGAPVIFGTFVSTLSMQSGAPTFGTPEAAMAIY
                     GAAQLARRMNMPFRTGGSLCASKIPDAQAAYESAQTLVPTVLAGTNFVLHAAGWMEGG
                     LAASYEKFVMDFDQLGAMHVLAKGVDFSENGQAMEAFHQVEPGGHFLGCPHTQANFES
                     AFYRSNLADNNSVEQWQEEGSKDMATRANALWKKMLAEYEAPPLDPAVDEALREFIDK
                     RSASMPDATY"
     gene            complement(2470..3051)
                     /locus_tag="VKU03_12640"
     CDS             complement(2470..3051)
                     /locus_tag="VKU03_12640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315783.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HLJ72160.1"
                     /translation="MAELGMAAARRPALDAPPVARRGVSIRLLPPEARFSLRLPMAAA
                     AAAGETAGFVLSMAINRCARKNDRWSARLGPNEWLLGGPENEGEAIADAIEAALAGAV
                     HALTDISHRHVAFEIAGPEAAALVNSGCPLDLSEGAFPAGCATRTLLGKAEIVLMRPG
                     PTPDFRLECWRSFSEYVHAFLVEAARDFPILEQ"
     gene            complement(3044..6034)
                     /locus_tag="VKU03_12645"
     CDS             complement(3044..6034)
                     /locus_tag="VKU03_12645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012112886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HLJ72161.1"
                     /translation="MKQEYRLPKGGRINRASPLSVSFNGRPLSGFAGDTMASILLAHG
                     QHLVGRSFKYHRPRGILSHGSDEPNALLSVDRGPGRVDPNNRASMVEARDGLATHSQN
                     HWPSLDFDVGAVNDALSPLFAAGFYYKTFMWPRAFWERVYEPAIRAAAGLGEAPTAPD
                     PDRYAHRHAHCDVLIVGAGPAGLAAALAASATQKQVILVDESAEPGGALLHDLSATID
                     GAPAGAWLDETLKTLRGRGNLAILARTTAFGYYNHNHVAMVERLTDHLAAPPADLARE
                     RLWQVRAREVVLATGAHERPLIFADNDRPGIMLAESVRAFINRYAVAPGRRIVFATNG
                     ASAYAAAADAKTAGLDVRMVDIRSESACGQEAAALRAMGCEVLTGHTIVGSTGRKRVS
                     GLIVAPVTSGGAVGARRTLACDCVGLSGGWTPAVHLFSQSRGKLRFEPAIDAFVPDRA
                     AQPVRVAGAARGAYDLSACLAEGFAAGTEAAGAPAAPPAHAASKTRTGFQPIRALPTD
                     RDAKRIKAFVDFQNDVTAKDIKLAIREGFESIEHVKRYTTTGMATDQGKTSNMNALLL
                     AADALDKPVPQVGTTTYRPPYTPVSFGAIVGPSRNALFDPVRKTPIHDWAAERGAMFE
                     NVSLWKRAWYFPQAGEDLDAAVARECKAARSAVGLFDGTTLGKIEVVGPDAAEFMNRI
                     YVNSWTKLEPGRCRYGLMLKEDGYILDDGVVARLTPDRFHVTTTTGGAARVLNHMEDY
                     LQTEWPHLNVFLTSVTEQWAVIAVQGPKARDVIAPFIEGADLSNEAFPHMAVRLCRCL
                     GAPCRLFRVSFTGELGYEINVPSDFGRAVWEAVYERGQAFGVTPYGTEAMHVLRAERG
                     FIIVGQETDGTMTPDDVGLGGMIAKAKPDFVGKRSLARPDLIAPGRKQLVGLLTDDPN
                     LVLDEGAQIVADPSQPIPMRMIGHVTSSYWSANCGRSIALAMIADGRNLMGRTLHATI
                     PAGFAAVTVSAPVFVDPKGERVHG"
     gene            complement(6031..>6250)
                     /locus_tag="VKU03_12650"
     CDS             complement(6031..>6250)
                     /locus_tag="VKU03_12650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606581.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sarcosine oxidase subunit delta"
                     /protein_id="HLJ72162.1"
                     /translation="PTQPAELDDKAWAEFLFIRSNPKGVHAERWIHQHGCQRWFNALR
                     DTVSDAFLETYEMGAKPKSRPDGAGTAR"
BASE COUNT         1072 a         2063 c         2063 g         1052 t
ORIGIN      
        1 gcacgcccat ttccggcagg ccctgggcct tgcgcgccat gatcgccgga tgcttttcgg
       61 tgacgatata ttggtgctgg accggaatca ccgggacgtt gattccgacc atccgcccgg
      121 tctggcgggc gaaattgccg gtcgccgcga ccacatgctc gcagacgatc tcgcccttgt
      181 cggtgacgac cttccactcg ccggaagccg tccgctcgat cgcggtgacg accgtgttgc
      241 ggttgatctc gccgccgccg ttgcgagcgc cgcgcgccaa agcctgggtc aaatcggccg
      301 gctggatata gccgtcctcg ggatgctgga tggcgccgac aattccgtcg atctcgcaga
      361 gcggccaaat ctccttgact tcgccgggcg tcaggaactt cacctcgacg ccgatcgtcg
      421 ccgcgacccc ggcatagtac ctgtactcgt cgagacgatc cttggtgcgg gcgagccgga
      481 tgttcgaaac ctgggcgaag ccgacattca tgccggtctc ggcggcaagg cgcttataga
      541 gcgccaccga atatttgtgg atctggccga ccgaatagct gacattgaac agcggcagca
      601 gcccggcggc atgccaggtc gagcccgatg tcagctcctt gcgctcgaca agcacgctgt
      661 cggtccaacc cttcttaacg agatgataga gggtcgagac gccgaccacg ccgccgccga
      721 tcaccaccgc ccgcgcttgg cttcgcatca atgtctccgc ttccctaagg cctgaagccc
      781 tgttttttcg gtcttttccg tcattgcgag cgaagcgaaa caatccattc gtagggttct
      841 accgatgaat gggtcgcttc gctcctcgca atgacggcca acattatccg cttagtacgt
      901 tgcatccggc atcgaggcgc tgcgcttgtc gatgaactcg cgcaacgcct catcgaccgc
      961 cggatcgagc ggcggcgctt cgtattccgc cagcattttt ttccacagcg cgttggcccg
     1021 cgtcgccatg tccttcgagc cttcttcctg ccattgctcg acggaattgt tgtcggcgag
     1081 attggagcgg tagaaggcgg attcgaaatt ggcctgggta tgcggacagc cgaggaaatg
     1141 cccgcccggc tcgacctgat ggaaggcctc catggcctgg ccgttttccg agaaatcgac
     1201 gcccttggcg aggacgtgca tggcgccgag ttgatcgaaa tccatgacga atttctcgta
     1261 ggatgcggcc agtccgccct ccatccagcc ggcggcgtgg aggacgaaat tcgttcccgc
     1321 caacacagtc ggaaccagcg tctgcgcgct ctcataggcg gcctgggcgt cgggaatttt
     1381 cgacgcgcag agcgagccgc cggtgcggaa cggcatattc atgcgccgcg ccaattgcgc
     1441 cgcgccatag atcgccatgg ccgcctccgg cgtgccgaaa gtcggcgcgc ccgattgcat
     1501 cgacagggtg gagacgaacg tgccgaaaat caccggcgcg ccgggccggg tgagctggca
     1561 gaaagccgtt cccgccatca cctcggccag gacctgcgtc agcgtcccgg ccaccgtcac
     1621 cgggctcatc gcgcccgaaa gaatgaacgg cgtgatgatg caagcctggt tgttgcgcgc
     1681 atagacctcc gccgcgccga gcatcgagga atcccagacc agcggcgaat tggcgttgat
     1741 caggctggtg cagaccgtgt tggactggac gaaatccgcg ccgaacagaa tctcgcacat
     1801 cgtcaccgtg tcttgcgcgc gctcgggctt ggtcaccgag cccatgaacg gcttgtcgga
     1861 atatttcata tgggcgtaga ccatatgaaa atgccgcttg ttcaccggca ggtcgacagg
     1921 ctcgcagacc gtgccgcccg aatgatggat gaagggcgtc gcataggaga gcttcacgaa
     1981 attgcggaaa tcctcgatcg tgccgtagcg ccggcccttg tcgaggtcgt gcacgaaagg
     2041 cgagccgtaa ttcggcgcga acaccgtatg attgccgccg atcgcgacgt cgcggccgcg
     2101 gttgcgcgcg cattggatgt agctcgccgg cgtcgtggcg cagagcttgc gggcgagccc
     2161 gcgcggaaac ctcacccgct cgccgcgaat gtcgcaaccc gcctccttga acagagccaa
     2221 tgcgcgcggg aattcgcgaa attcgatacc gacctcttgc agcaaggtct cggcattgcg
     2281 ctcgatcagc gccaggccct cctcgctcaa aatttcggtc agcgggatgt tgcgggtgat
     2341 gtacgggaca tgagcgacgg cgcgctgggc gcgcgccgcg cgccgcgcct cgcggccgcg
     2401 tcgcgcttcg tgggcaggag cgccggtcgc ggaaggttcg acggacatgg tttgctcctc
     2461 tgcttaagac tattgttcca ggatcgggaa atcgcgcgcc gcttcgacga ggaacgcgtg
     2521 aacatattcg ctgaatgagc gccaacactc gagccgaaaa tccggcgtcg gaccgggccg
     2581 catcaggacg atttcggcct tgccgagcaa agtccgggtc gcgcagccgg cggggaaggc
     2641 tccttccgag agatcgagcg ggcagccgga attgacgagc gccgcggctt ccggcccggc
     2701 gatttcgaaa gcgacatggc ggtgcgaaat atcggtcaaa gcgtgaacgg cgccggccag
     2761 cgccgcttcg atcgcatccg cgatcgcctc gccttcgttc tccgggccgc cgaggagcca
     2821 ttcattgggg ccgagccgcg ccgaccagcg atcgttcttc cgcgcgcagc gattgatcgc
     2881 catgctcagt acaaaccctg cggtctcccc cgccgccgcg gcggcggcca tcggcaggcg
     2941 cagcgaaaag cgcgcctccg gcggcaagag gcggatggag acgcctcgcc ttgcgaccgg
     3001 cggcgcatcg agcgccggcc gccgcgccgc cgccatgcca agctcagcca tggacgcgct
     3061 ctcccttcgg atcgacgaaa accggcgcgc tgaccgtgac ggcggcgaag cccgccggaa
     3121 tcgtcgcgtg caaagtccgg cccatcagat tgcggccgtc cgcgatcatc gccaaggcga
     3181 tcgagcggcc gcaattggcg ctccaatagc tcgacgtgac atggccgatc atccgcatcg
     3241 ggatcggctg gctgggatca gcgacgatct gcgcgccctc gtcgagcacg agattgggat
     3301 cgtcggtcaa caggccgaca agctgcttgc ggcccggcgc gatcagatcg ggccgcgcca
     3361 gcgagcgctt gccgacgaaa tccggcttgg ctttggcgat catgccgcca agcccgacat
     3421 cgtccggcgt catcgtgccg tccgtctcct ggccgacaat gatgaagccg cgctcggcgc
     3481 gcagcacgtg catcgcctcg gtgccgtaag gcgtgacgcc gaaagcctgg ccgcgctcgt
     3541 agaccgcctc ccagaccgcg cggccgaaat cggaggggac attgatctcg tagccgagct
     3601 cgccggtgaa ggagacgcgg aacagccggc acggcgcgcc gaggcagcgg cagagccgca
     3661 ccgccatatg cgggaaagcc tcgttcgaga gatcggcgcc ctcgatgaac ggcgcgatga
     3721 cgtcgcgcgc cttcggccct tgcaccgcga tcaccgccca ttgctcggtg accgaggtca
     3781 ggaacacgtt gagatgcggc cattcggtct ggaggtagtc ctccatatgg ttgagcacgc
     3841 gcgccgcgcc gccggtcgtc gtcgtcacat ggaaacggtc gggcgtaagg cgggccacga
     3901 cgccgtcatc gagaatatag ccgtcctcct tcagcatcag gccgtagcgg cagcggcccg
     3961 gctcaagctt ggtccaggaa ttgacgtaga tgcggttcat gaattcggcg gcgtcgggac
     4021 cgacgacctc gatcttgccg agcgtcgtgc cgtcgaacag gccgacggcg ctccgcgccg
     4081 ccttgcattc gcgcgccacc gccgcgtcga gatcctcgcc ggcttgcggg aaataccagg
     4141 cgcgcttcca cagcgagacg ttttcgaaca tcgcaccgcg ctcggccgcc caatcgtgaa
     4201 tcggcgtctt gcggaccgga tcgaacaggg cgttgcgcga aggcccgacg atcgcgccga
     4261 aactgaccgg cgtgtaaggc ggccggtacg tcgtcgtccc gacttgcggg acgggcttgt
     4321 cgagcgcgtc ggcggcgagg agaagcgcgt tcatattcga cgtcttgccc tggtcggtcg
     4381 ccatgcccgt cgtcgtgtag cgcttcacgt gctcgatcga ttcgaagcct tcgcggatcg
     4441 caagcttgat gtctttggcg gtgacgtcgt tctggaaatc gacaaaggcc ttgatccgct
     4501 tggcgtcgcg atcggtcggc agagcgcgga tcggctggaa gccggtccgt gttttcgacg
     4561 cggcgtgtgc gggcggggcg gcgggcgcgc cggcggcctc ggtcccggcg gcgaagcctt
     4621 cggcgaggca agccgaaaga tcgtaggcgc ccctcgccgc gcccgcgacc cgtaccggtt
     4681 gcgcggcgcg gtccggtacg aacgcgtcga tcgccggctc gaatcgcagc ttgccgcgcg
     4741 attgcgagaa gagatggacc gccggcgtcc agccgccgga aagaccgacg caatcgcaag
     4801 caagcgtccg ccgcgcgccg accgcgccgc cgctcgtaac cggcgcgacg atcaagccgc
     4861 tgacccgctt ccgcccggtc gagccgacaa tcgtatggcc ggtcaaaacc tcgcagccca
     4921 tggcgcgaag cgccgcggct tcctggccgc aggccgactc gctgcggatg tcgaccatcc
     4981 gcacatcgag ccccgccgtt ttggcgtccg ccgcggccgc gtaagcagac gcgccgttcg
     5041 tcgcgaaaac gatgcggcgg ccgggcgcaa ccgcatagcg attgataaac gcgcggacgc
     5101 tctccgccag catgatgccc ggccggtcat tgtcggcgaa gatcaacggc cgttcatgcg
     5161 cgccggtcgc cagcacgacc tcgcgcgcgc gaacctgcca caggcgctcg cgggcaaggt
     5221 ccgccggcgg cgcggcgaga tgatcggtca agcgctcgac catcgcgaca tgattgtggt
     5281 tgtaatagcc gaacgccgtc gtgcgcgcca ggattgcgag attgcctcgg ccgcgcagag
     5341 tcttcagcgt ctcgtcgagc catgccccgg ccggcgcgcc gtcgatcgtc gcgctcaggt
     5401 cgtgcagcag cgccccgccg ggctccgcgc tttcgtcgac gaggatgacc tgtttttgcg
     5461 tcgccgaggc ggcgagcgcc gcggcgaggc cggcgggtcc ggcgccgaca atgagcacgt
     5521 cgcaatgggc gtggcgatgg gcgtagcggt cgggatcggg cgccgtcggc gcttcgccga
     5581 gaccggcggc ggcgcgaatg gccggttcat agacgcgctc ccagaaggcg cgcggccaca
     5641 tgaacgtctt atagtagaag ccggcggcaa aaagcggcga cagcgcgtcg ttgaccgcgc
     5701 cgacatcgaa atcgagcgat ggccaatggt tctgcgaatg cgtggcgagc ccatcgcgcg
     5761 cttcgaccat cgaggcgcga ttgttgggat cgacgcggcc aggcccgcga tcgacgctga
     5821 gcagcgcgtt cggctcgtcc gagccatgcg agagaatgcc gcgcggccgg tgatatttga
     5881 acgagcggcc gacgagatgc tgaccgtgcg cgagcaggat cgaggccatc gtatcgccgg
     5941 cgaaacccga gagcggacgg ccattgaagc tgacgcttaa tggggaggcg cggttgatgc
     6001 gcccgccttt cggcagccgg tattcctgct tcatcgcgcc gtccccgcgc cgtcaggacg
     6061 gctcttcggc ttggcgccca tttcgtacgt ctcgagaaac gcgtcgctca ccgtgtcgcg
     6121 cagcgcgttg aaccagcgct ggcagccatg ctggtgaatc cagcgctcgg cgtgaacccc
     6181 cttcggattg gagcggatga acaggaattc cgcccaagcc ttgtcatcca actcggccgg
     6241 ctgcgtcggc
//
LOCUS       DATGJW010000422         3889 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1014201, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000422 DATGJW010000000
VERSION     DATGJW010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3889)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3889)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3889
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1014201"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(242..697)
                     /locus_tag="VKU03_12655"
     CDS             complement(242..697)
                     /locus_tag="VKU03_12655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR02301 family protein"
                     /protein_id="HLJ72163.1"
                     /translation="MIRRFAGLLLAAALLAAPFGAAAQKIAPVERAATEKGKEKEKAP
                     NPAPEPSPPPYEPQLLRLSEVMGALTYLRGLCGERDAEIYRAKMAALLAAEATSDARK
                     DRLAGAYNRGFQGYALTYRTCTPAARVVIARFLDEAARVSKDLANRYGG"
     gene            complement(694..1140)
                     /locus_tag="VKU03_12660"
     CDS             complement(694..1140)
                     /locus_tag="VKU03_12660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HLJ72164.1"
                     /translation="MTAEGDRLYPARPILAVSLAVFRQGRALIARRARAPLKGLYSLP
                     GGRVEIGETLQEAALRELDEEVGLRAEILAFNDHVEQIVRDSQGVRAHYVIASFVGRW
                     LAGEALTSAEADRVMWIDPIAPISEPTTPGLAAILAKAARLAEAFA"
     gene            complement(1137..1892)
                     /locus_tag="VKU03_12665"
     CDS             complement(1137..1892)
                     /locus_tag="VKU03_12665"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927772.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72165.1"
                     /translation="MTQAQTNAPRRAMLLTGASRGIGHATVKRFSSAGWRVITCSRHP
                     FPGEECPWMAGPQDHLQIDLADPADTRRAIAEVRERLEEGRLDALVNNAAISPKGLGG
                     SRLGFFDTELDDWLTVFQVNFFAPIMLARGLAAELEKARGSIVNVTSIAGSRVHPFAG
                     AAYATSKAALAALTREMAADFGPRGVRVNAISPGEIDTAILSPGTDKIVAQIPMRRLG
                     SPEEVAKAIYFLCTDQSSYVHGAEIHINGGQHV"
     gene            2664..3395
                     /locus_tag="VKU03_12670"
     CDS             2664..3395
                     /locus_tag="VKU03_12670"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72166.1"
                     /translation="MTRTSKRRHQVMERTSGVSSAEEMPTRAAATTSTNVAAGTTGPA
                     PMARGYAYNSGYGYAPGYAYGAGYAYGAAGYGYAPGYAYEPSYTYGYPAPGYAPGYAY
                     APTYASSPSYAYDVSYDYRPGYAYGTPAYGPNWGAPGQPVYGPVTQPAPVAPAQPAAT
                     VQPVATAPAPVVAQPATTVAVAQPVAPAPAIVEGRAAFVGDDTFTYRPTPSDQYTNGN
                     GGHWDAFEDESPAGQFSAQQIRAGR"
     gene            complement(3447..>3889)
                     /gene="pdxH"
                     /locus_tag="VKU03_12675"
     CDS             complement(3447..>3889)
                     /gene="pdxH"
                     /locus_tag="VKU03_12675"
                     /EC_number="1.4.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004733 - pyridoxamine phosphate oxidase
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_process: GO:0008615 - pyridoxine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase"
                     /protein_id="HLJ72167.1"
                     /translation="GPEGFVFYTNSESAKGQELAQNMQAALLFHWKSLRRQVRVRGIV
                     EKVTTSESDAYFNSRPRDSRIGAWASQQSRPLESRFALEKAIAFYAAKFAIGDVPRPP
                     YWRGYRLTPLAFEFWRDRLFRLHDRIQFTRAAVDAAWSRQRLYP"
BASE COUNT          649 a         1293 c         1334 g          613 t
ORIGIN      
        1 gagcaggcgc tgtcgggacg gctgatccgg ccgcaatcgc gctatatcgg gccgcgcccc
       61 ggcgaggcgg cgtgacgtac tcatcctccc ccgtcgaaga cgggggaggg ggaccgcgcg
      121 tcagcgcggt ggagggggcg cgccggcggt tgggatttaa tcctgacagg ccgaagcccc
      181 ctccaccatg cttcgcctgg tcccctccct gcgcttcgcg gggaaggata gctacgccgc
      241 cttatcctcc gtagcgattc gccaaatcct tcgacactcg cgcggcttcg tcgagaaacc
      301 gcgcgatcac cacgcgcgcc gccggcgtgc aggtccggta ggtcagcgca tagccctgga
      361 acccgcgatt ataggcgccg gcgagccggt ccttgcgggc gtcgctcgtc gcttcggcgg
      421 cgagcagggc ggccattttg gcgcggtaga tctcggcgtc gcgctcgccg cacagaccgc
      481 gcagataggt cagcgcgccc atcacctcgc tcaggcggag caattgcggc tcgtagggcg
      541 gcggcgacgg ctccggcgcc gggttcggcg ctttctcctt ctcctttccc ttctcagtcg
      601 cggcgcgctc gactggcgcg attttctggg cggccgcgcc aaagggcgcc gcaagcaacg
      661 ccgcggccaa aaggagaccg gcgaagcgcc gaatcacgcg aaggcctcgg ccagccgcgc
      721 cgccttggcg agaatcgccg caaggcccgg cgtcgtcggc tcgctgatcg gcgcgattgg
      781 gtcgatccac atcacgcgat cggcttcggc gcttgtcagc gcttcgcccg ccagccagcg
      841 cccgacgaag gaagcgatga cgtaatgcgc gcggaccccc tggctgtcgc gcacgatctg
      901 ctcgacatgg tcgttgaagg cgaggatttc cgcgcgcaag ccgacttctt catcgagctc
      961 gcgcagcgcc gcttcctgca aagtctcgcc gatctcgacg cggccgcccg gcagcgaata
     1021 gaggcccttc agcggcgccc gcgcgcgccg cgcgatcagc gcccggcctt gcctgaacac
     1081 ggccagcgag acggcgagaa tcggccgcgc cggataaagg cgatcgccct ccgccgtcat
     1141 acgtgctggc cgccattgat atggatctcg gcgccgtgca cgtagctcga ttggtcggtg
     1201 cagaggaaat agatcgcctt ggcgacttcc tccggcgagc cgaggcggcg catcggaatt
     1261 tgcgcgacga tcttgtcggt gccgggcgag aggatggcgg tgtcgatctc gccgggcgag
     1321 atggcgttga cgcgcacgcc gcgcgggccg aaatcggcgg ccatttcgcg cgtcagcgcc
     1381 gccagcgccg ccttcgacgt cgcataggcg gcgccggcga aaggatggac gcgcgagccg
     1441 gcgatcgagg tgacgttgac gatcgagccg cgcgcttttt cgagctccgc ggcgaggccg
     1501 cgcgcgagca tgatcggcgc gaagaaattc acctggaaga cggtgagcca atcgtcgagc
     1561 tcggtgtcga aaaagccgag ccggctgccg ccaagcccct tcggcgagat cgccgcgttg
     1621 ttgaccaagg cgtcgagccg tccctcttcg aggcgctcgc gcacttcggc gatggcgcgc
     1681 cgcgtgtcgg ctggatcggc gaggtcgatc tgcagatgat cctgcgggcc ggccatccac
     1741 gggcattcct cgccgggaaa ggggtggcgc gagcaggtga tgacgcgcca gcccgccgag
     1801 gaaaaccgct tgacggtcgc atggccgatg ccgcgcgaag cgccggtcag cagcatggcg
     1861 cggcgaggcg cattggtttg agcttgggtc atctcgctag agcggcgttg gagcgctgga
     1921 gcgtcatagc acatccggcg cggggccgcg cgataagtgc gacgccccgg ggccgctgcg
     1981 cgaaacggcg attcggcgaa gcgcccgccg gtcagtaatt gtcgctttcg cgcggcggct
     2041 ttgtggcgaa gcggcgccgg gcgatcacgc ctgttcaaca gcgctcacga cgcgatgacg
     2101 gcgtcccgcg caaggggaaa aacgccgaat ttgcagcctt tgtttgtgag gttctgcctg
     2161 gagccgcttc acagcctctg aggcgttacg cggaaacctt cgtcgcgcgc gtcagtttgt
     2221 caacgcgcag acgccaagcc gcgccagtgt tcggccggcg aaaaagcggg gtggcgaccc
     2281 aaggaccatt tgaggagacc acgatgagaa ggattgttct cgcagcaagc gccgcggctc
     2341 ttgtcgccgc tggcgcgccc gccgcgatgg cccagatgca agcgccggcc ggtcaggcgg
     2401 gcttcaacag cgatgtgcgc tatgggccga attggggcgc gccgaattcg ccctcctacg
     2461 ggccgaaaac ctccgcctcg acatgggcaa acccgccggc gacgaacgcc tcggcggccg
     2521 tgcagacaag cgccacgcct atggaaacga gggcggccgc gaccgaggag cgtcgacctg
     2581 agatgtcgcg tcgcatggcg cgaatggaga ggcggccgat ggaaaggcgg gcgggggctg
     2641 aagagcgtca gccggaaacc ggcatgacca gaacttcaaa gcgccgccac caggtcatgg
     2701 agcggaccag cggggtttcc tcggccgagg aaatgcccac acgcgctgcg gcaacgacca
     2761 gcacgaatgt cgcggccgga accacaggcc cagcgccgat ggcgcgtggc tacgcttata
     2821 actcgggcta tggctatgcg cccggctacg cttacggcgc cggctatgcg tatggcgccg
     2881 ccggctacgg ctatgcgccc ggctacgcct acgagcccag ctatacttat ggctacccgg
     2941 ctcctggcta cgcgcccggt tatgcctatg ctccgaccta tgcgtccagc cccagctacg
     3001 cctatgacgt gagctacgat taccggcccg gctatgctta cggcacgccg gcctatgggc
     3061 cgaattgggg ggcccctggc cagcctgtct atggcccggt gacccagccg gcgcctgttg
     3121 cgcctgcgca gccggcggcg accgtacagc ccgtagcgac ggcgcctgcg ccggtcgtcg
     3181 ctcaacccgc gacgactgtg gcggtggctc agccggtcgc gccggcgccg gccatcgtcg
     3241 agggccgggc ggcctttgtc ggcgacgata cattcaccta tcgtccgacg ccgtctgatc
     3301 aatacaccaa tggcaatggc ggccattggg acgcttttga ggacgaaagt ccggccggcc
     3361 aattcagcgc ccagcagatc cgcgccggcc gctaaaagcg cgccggagcc ggagcgatcg
     3421 gcgggaggct tcgcgcctcc cgctttttat ggatagagcc gctggcggct ccaggccgca
     3481 tcgacggcgg cgcgcgtgaa ctggattcgg tcgtgcaggc ggaacagccg gtcgcgccag
     3541 aattcgaagg ccagcggggt caagcggtag ccccgccaat agggcggcct cgggacgtct
     3601 ccaatcgcaa atttcgcggc atagaaggcg atcgccttct caagggcgaa gcgactctcc
     3661 agcgggcgcg attgctggct cgcccaggcg ccgatacgcg aatcgcgcgg ccggctgtta
     3721 aagtaggcgt cagattcgga tgtggttact ttttctacaa tgccgcgcac tcgaacctgg
     3781 cggcgcagcg atttccaatg aaacagcaga gcagcctgca tattctgcgc caattcctgg
     3841 cccttggcgc tctcggaatt ggtataaaaa acaaaccctt ccgggccgt
//
LOCUS       DATGJW010000423         3324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1220677, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000423 DATGJW010000000
VERSION     DATGJW010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3324
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1220677"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..534)
                     /locus_tag="VKU03_12680"
     CDS             complement(<1..534)
                     /locus_tag="VKU03_12680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459319.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HLJ72168.1"
                     /translation="MKAKIRKLITVIEETRAEMDRAVEPPTRRAAALAVIENPFAGRY
                     VEDLSELIDIGEELGDLLGRRAIAALGIEGPAAESYGKAAAVGENGELEHAAAVLHPK
                     LGAPLRKVLGKGAALIPSSKKRGGPGVALDIPLGHKDAAFVRSHFDGMEVRVSDAPRA
                     NEIVVAVAVTDSGRPLPR"
     gene            complement(531..1115)
                     /locus_tag="VKU03_12685"
     CDS             complement(531..1115)
                     /locus_tag="VKU03_12685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008876084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid synthesis family protein"
                     /protein_id="HLJ72169.1"
                     /translation="MPSVEERKRVVAVEDIYHEGGPVAAKPYRRAYILSVVRNPFAGR
                     YVEEIVGFSDDLKPLGLDMARALIAALGGDPKAVEGYGKGAIVGTAGEIEHGALWHAP
                     GGYGMREALGGAKAIVPSAKKVAGPGARLDVPITHVNASYVRSHFDAIEVGIADAPRA
                     GEILLALAMTAGPRVHDRAGGLKASEIKGVDGLR"
     gene            complement(1102..2136)
                     /locus_tag="VKU03_12690"
     CDS             complement(1102..2136)
                     /locus_tag="VKU03_12690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0280 family protein"
                     /protein_id="HLJ72170.1"
                     /translation="MRAPPQYRRLADGRRHFNDGPIDLVIEAFGEPDAVESAYAAAWR
                     RFQTILDELCAELPLLRAQAAPDGPRPAGPVARRMAAAVAPFCDAHFITPMAAVAGAV
                     AEEILQAMTGAAPLRRAYVNNGGDIALHLSAGERFSIGMVDRPDRPSLIGAATIAARD
                     PIRGVATSGWRGRSFSLGVADAATILARTAASADAAATLVANAVDLPGHPAIRRAPAS
                     SRDPQSDLGDRLATVGVGALTPAEIDEALARGLAEAERLRALGAIAAAALRLCGETRL
                     CGVEARRYNRLDGQREAVNNPLRHCERSEAIQAAPGPLQTPKGLLRRFAPRNEGTGDG
                     RQGAERHAVG"
     gene            complement(2133..>3324)
                     /locus_tag="VKU03_12695"
     CDS             complement(2133..>3324)
                     /locus_tag="VKU03_12695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019196175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="6-hydroxynicotinate reductase"
                     /protein_id="HLJ72171.1"
                     /translation="TTYPDYKPAPFIVSSKVDGVDMVTVVTEGIYSYCGVKVKIDTDR
                     HLGAECAAVRAQGEPVGHVTTGEYGSQMLSLGGVNHLTHGGKKEGRVTCETLLDLCNR
                     KAVELTVDGGATVVVEAGKPPLVNGRKEERMRVGCGSATIGMFAKQWYGKVDEVVVVD
                     DHITGVLSEHQAGKMLGAKETGVKMKGRRSTPGRYFHVAEPGTGWGGANISDPLDILA
                     GFDPKVAWPGLTMLMVSTTGEQHAFYRLDENLRPVEEALPESLRVSVERIRENCEPAL
                     CSVLFMAGAGGSLRAGVTENPVRLTRSVRQALTTVTAGGAPVVVWPGGGITFMVDVTL
                     LPDNAFGYVPTPAIVAPIEFTLRLDDYAALGGHVDHVRPLSDVVENHAKRQNAAAAPA
                     RRQA"
BASE COUNT          497 a         1160 c         1138 g          529 t
ORIGIN      
        1 tcggggtagg ggcctgccgg aatcggtcac cgccactgcg acgacgatct cgttcgcgcg
       61 cggcgcgtcc gagaccctca cttccatccc gtcgaaatgg ctgcgcacga aagccgcgtc
      121 cttatggccg agcgggatat cgagcgcaac gcccgggccg ccgcgcttct tcgacgacgg
      181 gatcagcgcc gcgcccttgc cgaggacttt gcgcaaaggc gcgccgagct tcggatgcaa
      241 cacggcggcg gcgtgttcga gctcgccatt ctcgccgacc gccgccgcct tgccgtagct
      301 ttcggcggcc ggtccctcga tcccgagcgc cgcgatggcg cggcgcccga gcagatcgcc
      361 gagctcctcg ccgatatcga tcaattcgga aagatcctcg acatagcggc cggcgaacgg
      421 attttcgatc accgcgagcg ccgcagcgcg gcgcgtcggc ggctcgacgg cgcggtccat
      481 ctcggcgcgc gtctcttcga tcacggtgat gagcttgcgg attttggctt tcatcggagc
      541 ccgtccacgc ctttgatctc ggaagctttc agcccgccgg cgcggtcatg cacgcgcggg
      601 ccggccgtca tcgccaaggc gaggaggatc tcgccggcgc gaggcgcgtc ggcgatcccg
      661 acctcgatcg cgtcgaaatg gctgcgcacg tacgaggcgt tgacatgggt gatcggcacg
      721 tccagccttg cgcccggacc ggcgactttc ttcgcggagg gcacgatcgc cttggcgccg
      781 cccagcgctt cgcgcatgcc gtagccgccc ggcgcgtgcc acagcgcgcc atgttcgatc
      841 tcgccggccg tcccgacgat cgcgcccttg ccatagcctt cgaccgcctt gggatcgccg
      901 ccaagcgccg cgatcaaggc gcgcgccatg tcgaggccga gcggcttcag gtcgtccgag
      961 aagccgacaa tctcctcgac atagcgcccg gcgaaggggt tcctgacgac ggagaggata
     1021 taggcgcggc ggtacggctt ggcggcgacc gggccgccct cgtgatagat atcctcgacc
     1081 gcgaccacgc gctttcgttc ttcaaccgac ggcatggcgt tcggctcctt ggcggccatc
     1141 gcctgtacct tcattgcgag gagcgaagcg acgaagcaat cccttcggcg tctgtaacgg
     1201 tcccggcgcc gcttggattg cttcgcttcg ctcgcaatga cgaagcgggt tattgacggc
     1261 ctctcgctgc ccgtccagcc ggttgtatcg cctcgcctcg acgccgcaaa gccgcgtttc
     1321 gccgcagagt cgcaaggcgg cggccgcgat cgctcccagg gctctgagcc gctcggcctc
     1381 cgcgagcccg cgcgccagcg cttcgtcgat ctccgccggc gtcagcgcgc cgacgccgac
     1441 cgtcgccagc ctgtcgccca aatcgctctg cggatcgcgc gatgaggccg gcgcgcgccg
     1501 gatcgccgga tggccgggaa ggtcgacggc gttggcgacg agcgtcgcgg cggcgtcggc
     1561 ggaagcggcc gtccgcgcga ggatggtcgc ggcgtcggcg acgccgagcg agaagctgcg
     1621 gccgcgccag ccggacgtgg cgacgccgcg gatggggtcg cgcgccgcga tcgtcgccgc
     1681 gccgatgaga ctgggccggt ccggccggtc gaccatgccg atcgaaaacc gctcgccggc
     1741 ggaaagatgc agcgcgatgt cgccgccatt gttcacgtaa gcgcggcgaa gcggcgcggc
     1801 gccagtcatc gcctgaagaa tctcctcggc caccgccccg gccaccgccg ccatcggcgt
     1861 gatgaaatga gcgtcgcaaa agggcgcgac cgccgccgcc atgcgccggg cgaccgggcc
     1921 ggcggggcgg gggccgtcag gcgccgcctg ggcgcgcagc aggggcaatt cggcgcagag
     1981 ctcgtccaga atcgtctgga agcgccgcca agcggcggcg taagccgatt cgacggcgtc
     2041 cggctcgccg aacgcctcga tgaccaggtc gatcggcccg tcgttgaaat ggcgccggcc
     2101 atcggccagc cggcgatatt gcggcggcgc tctcatgctt gcctccgcgc cggcgccgcc
     2161 gccgcgtttt gccgcttggc gtggttctcg acgacgtcgc tgagcggccg cacatggtcg
     2221 acatggccgc caagggcggc gtaatcgtcg agccgcaagg tgaattcgat cggcgcgacg
     2281 atggccgggg tcggcacata gccgaacgcg ttgtcgggca gaagcgtcac gtcgaccatg
     2341 aaggtgatgc cgccgcccgg ccagacgacg actggcgcgc cgccggcggt gaccgtggtc
     2401 aaagcctgcc gcaccgagcg ggtaaggcgc acagggtttt ccgtgacgcc ggcgcgcagc
     2461 gagccgccgg cgccggccat gaacaggacc gagcacagag ccggctcgca attctcgcgg
     2521 atgcgttcaa ccgagacgcg gaggctctcc ggcagcgctt cttcgacggg ccgcaaattt
     2581 tcgtcgagcc ggtagaaggc gtgctgctcg cctgttgtcg agaccatcag catcgtcagg
     2641 ccgggccagg cgactttcgg atcgaagccg gcgaggatgt cgagcggatc ggaaatattg
     2701 gcgccgcccc agccggtccc cggctcggcg acatggaaat agcggcccgg cgtcgagcgc
     2761 cggcctttca tcttgacgcc ggtctccttc gcgccgagca ttttgccggc ctgatgctcg
     2821 gagaggacgc cggtaatgtg gtcgtcgacg accacgactt cgtcgacctt gccgtaccat
     2881 tgcttggcga acatgccgat cgtcgccgag ccgcagccga cccgcatgcg ctcttccttc
     2941 cgcccgttga cgagcggcgg cttgccggcc tcgaccacca ccgtcgcgcc gccatcgacg
     3001 gtcagctcga cggcttttcg gttgcagaga tcgagcaagg tctcgcaggt gacgcggccc
     3061 tccttcttgc cgccatgggt caggtggttg acgccgccga gcgacagcat ttgcgagccg
     3121 tattcgccgg tcgtgacatg accgaccggc tccccttgcg cgcgcaccgc cgcgcattcc
     3181 gcgccgagat ggcgatcggt gtcgatcttc actttgacgc cgcaatagga atagatgccc
     3241 tcggtgacga ccgtcaccat gtcgacgccg tcgaccttgg acgagacgat gaacggcgcc
     3301 ggcttgtagt cgggataggt cgtg
//
LOCUS       DATGJW010000424         2272 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_913286, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000424 DATGJW010000000
VERSION     DATGJW010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2272)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2272)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2272
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_913286"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1272)
                     /locus_tag="VKU03_12700"
     CDS             complement(<1..1272)
                     /locus_tag="VKU03_12700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013896402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carboxyl transferase domain-containing protein"
                     /protein_id="HLJ72172.1"
                     /translation="MAVIKSTADLSSDACRANQAAWAPLIAELDRRRAAAAEGGSRRA
                     RERHIARGKLLPRERVARLLDPGSPFLEIAPLAAFGMYEGDVHAAGLIAGIGRIEGTE
                     TMIVANDPTIKGGAYYPMTVKKHLRAQEIARENNLPCLYLVDSGGANLPHQAEVFPDR
                     EHFGRIFYNQATMSAAGIAQIAVVMGSCTAGGAYVPAMSDETIIVRNQGTIFLGGPPL
                     VKAATGEVVSAEDLGGADVHTRISGVADAMARDDAHALALARRAVANLNKRKRHELAM
                     KEPAEPLYSADELAGVTPVDLRKQYDVREIIARLVDGSELDEFKARYGTTLVTGFAHL
                     AGMPIGIIANNGILFSESALKGAHFIELCAQRSIPLLFIQNISGFMVGRQYEAGGIAK
                     DGAKMVTAVACAAVPKFTVIIGGSFGAGNYGM"
     gene            complement(1313..>2272)
                     /locus_tag="VKU03_12705"
     CDS             complement(1313..>2272)
                     /locus_tag="VKU03_12705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HLJ72173.1"
                     /translation="VKLNPETQVVATLGSKEGFANMAQAITAPGDVVLAPNPSYPIHA
                     FGFLMAGGVIRSVPADPTPNFFVAMERAVNHSIPKPIAVVVCYPSNPTAQVASLDFYK
                     DLVLFAKKHEIFVLSDLAYGEVYFDDEPPPSVLQAPGAFDVAVEFTSMSKTFSMAGWR
                     MGFAVGNERLLAALARVKSYLDYGAYTPIQVAAAAALNGPEDCIREMRATYKRRRDVM
                     VESFAQAGWPIEAPCASMFAWTPVPEKFRHLGSLEFSKLLIEKAEVAVAPGIGFGEHG
                     DDYVRLALVENEQRIRQAARGIRRFFESADATLHNVVQLKKKA"
BASE COUNT          393 a          745 c          752 g          382 t
ORIGIN      
        1 catgccgtaa ttgccggcgc cgaagctgcc gccgatgatc acggtgaatt tcggcacggc
       61 ggcgcaggcg acggcggtca ccatcttggc gccatccttg gcgatgccgc cggcctcgta
      121 ttgacgcccg accatgaagc cggaaatgtt ttggatgaag agcagcggaa tcgagcgctg
      181 cgcgcaaagc tcgatgaaat gcgcgccttt cagcgcgctt tcggaaaaga gaatgccgtt
      241 attggcgatg atcccgatcg gcatgccggc aagatgggcg aagccggtga ccaaggtcgt
      301 cccatagcgc gccttgaact cgtcgagctc cgaaccgtcg acaagtcgcg cgatgatctc
      361 gcgcacatcg tactgcttgc gcagatcgac gggcgtcacg ccggcaagct cgtccgcgga
      421 ataaagcggc tcggccggct ccttcatcgc gagttcatgc cgcttgcgct tgttgagatt
      481 ggcgacagcc cgccgcgcca aggcgagcgc atgcgcgtcg tcgcgcgcca tggcgtcggc
      541 gacgccggaa atccgggtat ggacgtcagc cccgcccaaa tcctcggccg agacgacctc
      601 gccggtcgcg gccttgacca ggggcgggcc gccgagaaag atcgtgcctt ggttcctgac
      661 gatgatcgtc tcgtccgaca tggccggcac ataggccccg cccgccgtgc acgagcccat
      721 caccaccgcg atctgggcga ttccggccgc cgacatcgtc gcctgattgt agaagatgcg
      781 gccgaaatgc tcgcggtccg gaaagacctc ggcctggtgc ggcaggttcg cgccgccgga
      841 atcgacgagg tacaagcaag gcaggttgtt ctcgcgggcg atctcctgcg cgcgcagatg
      901 cttcttgacc gtcatcgggt agtaggcgcc gcccttgatc gtcgggtcgt tggcgacgat
      961 catcgtctcc gtcccctcga tgcggccgat tccggcgatc agcccggccg catgcacgtc
     1021 gccctcatac atgccgaagg cggcgagcgg cgcgatttcg aggaagggcg agcctgggtc
     1081 gagcaaccgc gccacccgct cgcgcggcag gagcttgccg cgcgcgatat ggcgctcgcg
     1141 cgcgcggcgc gagccgccct cggccgcggc ggcgcggcgc cgatcaagct cagcgattag
     1201 cggcgcccag gccgcttgat tggcccggca ggcgtcggac gaaagatcgg cggtgctttt
     1261 gatgacggcc acgaaaacgg cttctcgtta ggcgcgctcc gcttgcgtgt tcctacgcct
     1321 tcttcttcaa ctgcacgaca ttgtgcagcg tcgcgtcggc gctctcgaaa aagcgcctta
     1381 tgccgcgcgc ggcctggcgg atgcgctgct cgttctcgac caaagccagg cgcacgtaat
     1441 cgtcgccatg ctcgccgaag ccgattcccg gcgcgaccgc cacttccgct ttctcgatca
     1501 gcaatttgga gaattccagg ctgccgagat ggcggaattt ctccggcacc ggcgtccagg
     1561 cgaacatcga ggcgcacggc gcctcgatcg gccagccggc ttgtgcgaag ctctcgacca
     1621 tcacgtcgcg ccggcgcttg taggtcgcgc gcatctccct gatgcaatct tccggcccgt
     1681 tgagggcggc cgcggcggcg acctggatcg gcgtataggc gccgtaatcg aggtacgatt
     1741 tgacccgcgc caacgcggcg agcagccgct cgttgccgac ggcgaagccc atgcgccagc
     1801 ccgccatcga gaaagtcttc gacatcgaag tgaattcgac cgcgacgtcg aaagcgccgg
     1861 gcgcctgcag cacggaaggc ggcggctcgt cgtcgaaata gacctcgcca taggcgagat
     1921 cggacaggac gaaaatctcg tgcttcttgg cgaacaggac gaggtccttg tagaaatcga
     1981 gcgaggcgac ctgggcggtc gggttcgacg ggtagcagac gacgaccgcg atcggcttcg
     2041 ggatcgagtg attgaccgcg cgctccatcg cgacgaagaa gttcggcgtc ggatcggccg
     2101 ggacggagcg gatcacgccg cccgccatca gaaagccgaa ggcatggatc gggtacgacg
     2161 ggttgggcgc cagcaccacg tcgcccggcg cggtgatcgc ctgggccatg ttggcgaagc
     2221 cctctttcga gccgagcgtc gcgacgacct gcgtctccgg attcagcttc ac
//
LOCUS       DATGJW010000425        12898 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_492796, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000425 DATGJW010000000
VERSION     DATGJW010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12898)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12898)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12898
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_492796"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..353)
                     /locus_tag="VKU03_12710"
     CDS             complement(<1..353)
                     /locus_tag="VKU03_12710"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72174.1"
                     /translation="MGSSRISYAQLLETNNLVQASADETYWLCVTRTVQESKLFPVPA
                     YMMLSYMMSYYRYPSLLRKIEQRMAAEEIGDRARNAGIKIQNPSMGWALPGFYLLGRE
                     WLINLGLIRPQDAVE"
     gene            complement(392..1135)
                     /locus_tag="VKU03_12715"
     CDS             complement(392..1135)
                     /locus_tag="VKU03_12715"
                     /inference="COORDINATES: protein motif:HMM:NF012609.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP-utilizing enzyme"
                     /protein_id="HLJ72175.1"
                     /translation="MAKLYIDGDKYAEPSASVVVDAYHAAIKDLVRRKLDEAGGDWAS
                     FVFDKSYDLVGAKDFAAIEKKLLATGHRFDWSAMISVRERPEAYKPATGVGEDIKTFS
                     FEHPEAKVAPPDADGREQRGIGDNVVKYPKNLVGVARYIRSSERVLELLASGVPADTV
                     AIIDDSGGTLTAPILEQFKGVICAGGTVRSHLGILTREYGIPCLMNAKVSGIKEGDRV
                     EIECTAPAKTAEAYQTGVEMTAKIWRLTQ"
     gene            complement(1262..2794)
                     /locus_tag="VKU03_12720"
     CDS             complement(1262..2794)
                     /locus_tag="VKU03_12720"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLJ72176.1"
                     /translation="MNLALLLDAAARSFPDHPAVSVGDERRYDYAAYGALSARLAGGF
                     AALGRRPGDRVVLAMANNAEYLAILFALWRAGLVAVPVSAKLHAREIAAIAADCGAEL
                     CLATPDLADALAKALESPVQLIVLGETEWRQLAAGEPKAAADRRGDDLAWIFYTSGTT
                     GRPKGAMLSHRNLMSMAVLYLADIDALGPDDCHIHLAAQSHATGLFGLSHIAKATHQV
                     LPPSGGFSPAELADLLRLHHNATFFVPPTGLRRMLRDPAFARAPVENIRTVLLGAAPV
                     YAADLKAGYEALGPRLWNGYGQGESPCTITAMAKPLLAAAIAAGDEARMASVGLARTG
                     IAVAVVDADDRPTPAGEIGEVVVRGDTVMAGYWRRPEETAAALRGGWLHTGDLGAMDE
                     RGFLTLLDRAKDLVISGGANIYPREVEDVLLEDAAVGEAAVIGVPDPEWGESVLALVV
                     PAGDAPVDLARLEALCLSRIARFKRPKYWRVVVNLPKNSAGKVLKRELRQSYAAEFGR
                     AK"
     gene            complement(2802..4025)
                     /locus_tag="VKU03_12725"
     CDS             complement(2802..4025)
                     /locus_tag="VKU03_12725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011415063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HLJ72177.1"
                     /translation="MREAWIVGVGMTAFGRFPSRPLRDLADEAARAALADAAASPQRV
                     QAIVFANATQGALEGQHGIRGQAALDGSGFGNAPVINVENACASASTALHLAETLVGS
                     GAYDCVLALGAEKMATPDPAAAMAAFEGSWDLSRRAETLAALAAMGRGAPPPEAETDA
                     PHSVFMDVYAAFCRHHMARFGTTQRQMAAASAKNHAHSVENPRAQYRRPFTIDEVLAA
                     RLIAWPLTLPMCSPISDGAAAALVCARPLARASRAVRIRASVLMAGDRRDPDRLEDHV
                     ARKAAARAYALAGLGPEDVDVVECHDATAFGEILQSEMLAFFPLGEGGGAAERGETRI
                     GGRIPFNPSGGLESKGHPIGATGLGQVFEIVSQLRGEAGARQVEGARIGLIENGGGLR
                     GIEEAAVAVTLLERA"
     gene            4127..4921
                     /gene="surE"
                     /locus_tag="VKU03_12730"
     CDS             4127..4921
                     /gene="surE"
                     /locus_tag="VKU03_12730"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:NF001490.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HLJ72178.1"
                     /translation="MSELAPPPRLSQHRILIVNDDGIDGTGLKMLEEMARAFTDDVWV
                     VAPDEEKSGFSHSISMTVPIRARKLDERHYAVKGTPTDCALLAIYEFMGEKPTALLSG
                     VNRGANLAEDITYSGTAAAAMEGALLGVRSIALSQVFTIGGPVHWATARRFAPPILER
                     LLTCDWEPGSFVNVNFPDAPPEAVTETRVTTQGRRLPGSFRPVRRVDERNFPYYWIKL
                     AYETGSLEAGTDLLAIAENAVSVTPMQLDLTSNTFRHYLDRTFKEG"
     gene            complement(4926..5681)
                     /locus_tag="VKU03_12735"
     CDS             complement(4926..5681)
                     /locus_tag="VKU03_12735"
                     /EC_number="3.1.3.48"
                     /inference="COORDINATES: protein motif:HMM:NF024744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004721 - phosphoprotein phosphatase
                     activity [Evidence IEA];
                     GO_function: GO:0004725 - protein tyrosine phosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-protein phosphatase"
                     /protein_id="HLJ72179.1"
                     /translation="MSEAYARRLDFEGAINFRDLGGYPAAGGRRTRWRRLFRADSLAD
                     LTRADLARLDALGLKGLVDFRTDLERRLKPNRLPGGARIRTLELGFLPAGTLEMLERV
                     RLGRIDTVELEQLVLGQYRKFGVEHVEEYRQALAFAAEPDNYPLLFHCTSGKDRTGFA
                     AAVLLLAVGAPREVVLADYDLTNRYRRDVAHLFGPQTSPEVIALLLSAQTCYLEAALD
                     EIDRAHGSFDAYLRKALGVDAAMRARLIDLLTE"
     gene            complement(5678..6415)
                     /locus_tag="VKU03_12740"
     CDS             complement(5678..6415)
                     /locus_tag="VKU03_12740"
                     /inference="COORDINATES: protein motif:HMM:NF018066.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetoacetate decarboxylase family protein"
                     /protein_id="HLJ72180.1"
                     /translation="MSGRGRLTLERLGYSMPADAPAYQAPPYHYRNAQAVSIKFETDR
                     DAALDILPEPLELIEPACANLSVYRYPFSTFGPYHEAILRLYARHDGKPLTYIQQIFV
                     DTEPPLLAGREVWGFPKKLARIEFARDRDMIVGKLERPAGVRLATVVMRPEQPVSQLG
                     SNGPTTGLRIIPSADPATTRPALAELVAADSQHHLREAWEGPASISFPDRSIFDPVDR
                     FPVRRIVKAVYMEYDITLPAGRVIARL"
     gene            complement(6427..7167)
                     /locus_tag="VKU03_12745"
     CDS             complement(6427..7167)
                     /locus_tag="VKU03_12745"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72181.1"
                     /translation="MAVLITGGAKGLGRAVARRFAKPRADIFMVYRQDEAAAAEAAAE
                     IAALGARPHPIRADVATPAGARLLIDHVAAKTDRLDLIVHGAVKVLVGPLLDVDPDAL
                     AAALTLNGASLVFLVQAARPLLKRGASIVFLSSRGSRQVVPGYGAIGAGKALAEALMR
                     YLVAELAPLGVRINAVAPGTLDTEAVRNLFGDGTDAFLASEAAGNPSGRNITHDDYLG
                     LIEFLASPEASMIQGQTIFVNGGQYVTA"
     gene            7456..8448
                     /locus_tag="VKU03_12750"
     CDS             7456..8448
                     /locus_tag="VKU03_12750"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ72182.1"
                     /translation="MQVKTLMAAAAALLATTALAGSAAAQEKSKTALKVAIFSVNGAS
                     PTIHTMIESATAAAKARGWTVETYDGNGDQVATNNQANTFITRGFDALINIASANTQM
                     GGVIANAAKAHIPFVSTFSGMVPGITVDIGSNNVADGVFSASALAARIDGEGEVLKMN
                     WTVLPALAERDAGFHAVVGEYKKLKVTEMEIKVPGQVDDTFNKVTDYLTGHKNVKAIW
                     MGWDEVGVAAARAVQQSHLEHKPFVVSMDGNDAAYDMIREGTPLWLTIAYDVRGMGVQ
                     AVQAVADAVDGKSFPQRQIYKKPCLITKETAPPKGQYPDFKTCVLYSADLPKSN"
     gene            8472..10043
                     /locus_tag="VKU03_12755"
     CDS             8472..10043
                     /locus_tag="VKU03_12755"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ72183.1"
                     /translation="MSATKAAPALSEPDGAGQAKAEIFLEVAGLVKQFPGTRALDRVD
                     LTIRRGEIHALLGENGAGKSTLIRQICGASQPTEGRILIEGAEVSLPSPQAAAALGIA
                     VVHQHFNLVPQLSVCENLLLSESLPRRAGLFVDWREAYRRARTLLTRVDLDLDPRIEV
                     RRLRPDEAAMVAVAKAIATNAKLIVLDEPTTALLPAEVSVLFAHMRRLAGKGHAFLYV
                     SHRLAEVFEIADRVTVLRDGRNAGAWTRQEMSRRAIIEAIVGARKSWDEDAGPAPAAL
                     GEVVLRAEALRGGRVAEMSFELRKHEILGVAGLPGSGAEEALDLLYGRYPKAGGRLSV
                     GGADVDFRSPRDAKAAGLALAPKDRHAESLLPGASVRENISLPNLGQYVADPVIRFIR
                     RRTERRDAERIAKSLSVKMSSIEAPIDSLSGGNQQKAVLGRWLASGAKIFMLNSPTAA
                     VDIGAKAEIYALIRRIAADGGAVIFTSAEVEEFPRVCRRVLVFRDGRIVGELVGKDAS
                     EANILNLAAGAGDEN"
     gene            10033..11031
                     /locus_tag="VKU03_12760"
     CDS             10033..11031
                     /locus_tag="VKU03_12760"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72184.1"
                     /translation="MRTSAKATAAVASSRTDFALWFARYGTLVSLVALLVVFSIARPD
                     VFPTVDNLLNIMNQISILGTMAFGLTVCLVMGLFDLSIAAMATLGGYVATFLLVQYPD
                     AIGVPEAVFISLAIAAAIGVVNGLIVSYLGISAFIATLATGSIITGAMLGISDSKTII
                     TGIPDAFMAIGQGAILGVSNPILIMLAVGLALWLLLEHTQVGRHLYAIGGSAEASRLS
                     GLAVKRYAPFALAICAACAGLGGLMAAAVLGAGRPQGVGDGYLLNAFAAVFIGASSLR
                     PGKFHILGTLIGVLLIGVINNGLSIMGVPTYWQYIVQGVLLVAALFSAGVLTMRRH"
     gene            11274..12335
                     /locus_tag="VKU03_12765"
     CDS             11274..12335
                     /locus_tag="VKU03_12765"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002718599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLJ72185.1"
                     /translation="MRIAQISPLTEAVPPKLYGGTERVISWLTEELVALGHDVTLFAS
                     GDSTTAARLEPMWPRALRLDGSVRDPNALHMTMIEKVCRRAGEFDLLHFHLDYYPFSA
                     MSRQPTPFLTTLHGRLDLPEHEPVFSTFSAIPVVSISHAQRRPALQARWAGTVHHGMP
                     ETLLKPEPAKASYLAFLGRISPEKRVDRAVHIAERCGLPLKVAAKVDPVDQDYFNENI
                     RPLFAKPHVDFIGEIADAQKSEFLSGAIALIVPIDWPEPFGLVMIEAMACGAPVIAFN
                     RGAAPELVEDGVTGFVVEDEISAVGAVHQLARLSRETIRKRFEERFTARRMAREYLKI
                     YRELLGAATQRPRIASVSG"
     gene            complement(12332..>12898)
                     /locus_tag="VKU03_12770"
     CDS             complement(12332..>12898)
                     /locus_tag="VKU03_12770"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72186.1"
                     /translation="LIFAGCIVLFQLANAAMLPLAAGLMTLRSSKDAAMLVAVAILGP
                     QLVVALLSPWVGRRAEQWGRRPLLVLCFAALTIRALFFSYSANPTLLMAVQLLDGVCG
                     AVLGVLAPLIVADLAGDTGHFNLAQGAVGCAVGVGAAISTTAAGYVSDRLGGPAAFLA
                     MAAIGLLGLTAARFAMPETRSGAGGGEA"
BASE COUNT         2176 a         4299 c         4307 g         2116 t
ORIGIN      
        1 tcctcgaccg cgtcctgcgg gcggatgagg ccgaggttga tgagccattc gcggcccaag
       61 agatagaagc ccggcagcgc ccagcccatc gatggattct ggatcttgat gccggcgttg
      121 cgggcgcggt cgccgatctc ctcggccgcc atgcgctgct cgatcttgcg caggagcgag
      181 gggtagcgat agtacgacat catataggag agcatcatat aggcgggaac cgggaacagc
      241 ttggattcct gcaccgtgcg ggtgacgcag agccaataag tctcgtcggc gctggcctgg
      301 acgagattgt tggtctcgag cagttgcgcg taggaaatgc ggctcgaacc catttctgtg
      361 atcctccccc ggaaaggcgt ttgccggagc gctattgggt caggcgccag atcttggccg
      421 tcatctcgac gccagtctgg tacgcttcgg cggtcttggc cggcgccgtg cattcgatct
      481 cgacccgatc gccttccttg atcccgctga ccttggcgtt catcaggcag ggaatgccgt
      541 attcgcgcgt caggatgccg agatgcgagc gcaccgtgcc gccggcgcaa atcacgccct
      601 tgaactgttc gaggatcggc gccgtcagcg tgccgccgct gtcgtcgatg atggcgacgg
      661 tgtcggccgg cacgccggag gcgagcagtt cgagcacgcg ttcgctcgag cggatgtagc
      721 gcgcgacgcc gacaaggttc ttgggatatt tgacgacatt gtcgccgatg ccgcgctgct
      781 cgcggccgtc ggcgtccggc ggcgccactt tggcctcggg gtgctcgaag ctgaacgtct
      841 tgatgtcctc gccgacgccg gtcgcgggct tataggcttc ggggcgctcg cgcaccgaga
      901 tcatcgccga ccagtcgaag cggtggccgg tcgccagcag cttcttttcg atggccgcga
      961 aatccttggc gccgacaaga tcgtaagatt tgtcgaagac gaagctcgcc cagtcgccgc
     1021 cggcctcgtc cagcttgcgc cgaacaaggt ccttaatcgc ggcgtgatag gcgtcgacca
     1081 cgaccgaagc gctcggctcg gcgtatttgt cgccgtcaat gtagagcttg gccatgtcgc
     1141 ccgcctccct ccccgtccgg acgcttttgg gaacgcgttt ccgcgcgccc gattagccgc
     1201 gaggatgacg ccgcccgccg gggctgtcaa caagatgtta ggacaatatt gtagaacaga
     1261 tttatttggc gcggccgaat tccgcggcgt agctctggcg caattcgcgc ttgagcacct
     1321 tgccggcgct gttcttcggc aggttgacga cgacgcgcca atatttcggc cgcttgaaac
     1381 gggcgatgcg gctgaggcag agcgcctcca accgggccaa atcgaccggc gcgtcgccgg
     1441 ccggcacgac gagagcgagg acgctttcgc cccattcggg atcgggcacg ccgatcaccg
     1501 ccgcttcccc aacggcggcg tcctccagca gcacgtcctc gacctcgcgc ggatagatgt
     1561 tggcgccgcc ggaaatgacc aggtccttgg cgcggtcgag cagcgtcagg aatccgcgct
     1621 cgtccatcgc gccgagatcg ccggtatgga gccagccgcc gcgcaaagcc gccgcggtct
     1681 cctccggccg gcgccaatag ccggccatca ccgtgtcgcc gcgcacgacg acttcgccga
     1741 tctcgccggc cggcgtcggc cgatcgtcgg cgtcgaccac cgccaccgcg atcccggtgc
     1801 gggcgaggcc gaccgaggcc atgcgcgctt cgtcgccggc ggcgatagcg gcggcgagca
     1861 gcggtttcgc catcgcggtg atcgtgcatg ggctttcgcc ctggccgtag ccgttccaca
     1921 ggcgcggccc caaagcctcg tagccggctt tgagatcggc ggcgtaaacc ggcgccgcgc
     1981 cgagcaggac ggtgcggatg ttctcgaccg gcgcgcgggc gaaagccgga tcgcgcagca
     2041 tccgcctcaa ccccgtcggc gggacgaaga aggtcgcgtt gtgatggagc cgcagcaaat
     2101 cggccaattc ggccggcgaa aagccgccgc ttggcggcag gacctgatgc gtcgccttgg
     2161 cgatatgcga gaggccgaaa aggccggtcg catgcgactg cgcggcgaga tggatgtggc
     2221 aatcgtccgg gccgagcgcg tcgatatcgg cgaggtaaag gacggccatg ctcatcaggt
     2281 tgcggtggct cagcatcgcg cctttcggcc ggccggtcgt gccgctcgta tagaaaatcc
     2341 aggcaaggtc gtcgccgcgc cggtcggcgg cggctttggg ctcgccggcg gcgagttgac
     2401 gccactccgt ttcgccgagg acgatcagct ggacgggcga ctccagagcc ttcgccagcg
     2461 cgtcggcgag atcgggcgtc gccaggcaga gctctgcgcc gcaatcggcg gcgatcgcgg
     2521 cgatctcgcg cgcatggagc ttggcgctga ccggaacggc gacgagcccg gcgcgccaca
     2581 gcgcaaagag aatcgccaga tattcggcat tgttggccat ggcgaggacg acgcggtcgc
     2641 cgggacgacg ccccagcgcg gcgaagcccc cggcgagccg cgccgagagc gcgccatagg
     2701 cggcgtaatc gtagcggcgc tcgtcgccga ccgagacggc gggatggtca ggaaagctcc
     2761 gcgccgccgc gtcaagcagg agagcgaggt tcatcggcgc tctacgcgcg ttcgagcagt
     2821 gtgacggcga cggctgcttc ctcgatcccg cgcaggccgc cgccgttctc gatgaggccg
     2881 atgcgcgcgc cctcgacctg ccgcgcgccc gcttcgccgc gcaactggct gacgatctcg
     2941 aagacctggc ccaagccggt cgcgccgatc ggatggcctt tcgattcaag gccgcccgac
     3001 ggattgaagg gaatgcggcc gccgatccgc gtctcgccgc gttcggcggc gccgccgccc
     3061 tcgccgagcg ggaagaaggc cagcatctcg ctttgcagga tttcgccgaa cgccgtcgca
     3121 tcgtggcact cgacgacgtc gacatcctcc gggccgaggc cggcaagcgc gtaggcgcgc
     3181 gccgccgcct tgcgggcgac gtgatcctcc agccggtcgg gatcgcggcg atcgccggcc
     3241 atcagcacgc tggcgcggat gcggaccgcg cgcgaagccc gcgccagcgg acgggcgcag
     3301 accaaagcgg cggcggcgcc gtcgctgatc gggctgcaca tcggcaaggt cagcggccag
     3361 gcgatgaggc gcgccgcgag cacctcgtcg atcgtgaacg ggcggcgata ttgggcgcgc
     3421 ggattctcga cggaatgcgc atggttcttg gcgctcgccg ccgccatctg gcgctgcgtc
     3481 gtaccgaacc tcgccatgtg gtggcggcaa aaggcggcgt agacgtccat gaagacgctg
     3541 tgcggggcgt ccgtttcggc ttcgggcggc ggcgcgccgc gccccatcgc cgcgagggcg
     3601 gcgagcgtct cggcgcggcg cgacaggtcc caactcccct cgaaagcggc catggccgcg
     3661 gccgggtcgg gcgtcgccat cttctctgcg ccgagggcga gcacgcaatc ataggcgccc
     3721 gagccgacca gggtctcagc gagatggagg gctgtcgagg ccgaggcgca ggcgttctcg
     3781 acattgatca ccggcgcgtt gccgaagccg cttccgtcca gcgccgcctg gccgcgaatg
     3841 ccgtgctggc cctccagcgc gccctgcgtg gcgttggcga agacgatcgc ctgaacgcgc
     3901 tgaggggagg ccgcggcgtc ggcgagcgcg gcgcgggccg cctcgtcggc gaggtcccgc
     3961 agcggccgcg acgggaagcg cccgaaggcg gtcatgccga cgccgacaat ccacgcctct
     4021 cgcatcgccg gctccttgcc tcgccgcgtt ttgtcataca ggattgtagt atgttcatcc
     4081 aaagcgacgc acgccgcaag ggcggccgca caaaaatcgg tgggccatgt ccgaactcgc
     4141 gccgccgccg cggctcagcc agcaccgcat cctgatcgtc aatgacgacg ggatcgacgg
     4201 gaccggcctc aaaatgctcg aagagatggc gcgcgccttt accgacgacg tttgggtcgt
     4261 cgcgccggac gaggagaagt ccggcttctc ccattcgatt tcgatgaccg tgccgatccg
     4321 cgcccgcaag ctcgacgagc gccattacgc ggtcaaaggc acgccgaccg actgcgccct
     4381 gctggcgatc tacgagttca tgggggagaa gccgacggcg ctcctttccg gcgtcaatcg
     4441 cggcgccaat ctcgccgagg acatcactta ttccggcacc gcggcggcgg cgatggaggg
     4501 cgccctgctc ggcgtccgct cgattgcgct cagccaggtc ttcacgatcg gcgggccggt
     4561 ccattgggcg accgcccggc gcttcgcgcc gcccatcctc gaaaggctgc tgacctgcga
     4621 ctgggagccc ggcagcttcg tcaatgtcaa tttccccgac gccccgcccg aggcggtgac
     4681 cgagacgcga gtgacgacgc aaggccgccg ccttcccgga tcatttcggc cggtgcgccg
     4741 cgtcgacgag cgcaatttcc cgtactattg gatcaagctc gcctatgaga ccggaagcct
     4801 cgaagccggc acggacctcc tcgccatcgc ggagaacgcc gtctcggtga cgccgatgca
     4861 gctcgacctg acgtcgaaca cgttccgcca ttatctggac cgcaccttca aggaaggctg
     4921 acccctcact ccgtcaagag gtcaatcagg cgcgcgcgca tcgcggcgtc gacgccaagc
     4981 gccttgcgca ggtaggcgtc gaacgagcca tgcgcgcgat cgatctcgtc gagcgcggct
     5041 tcgagatagc aagtctgagc ggaaaggagc aaagcgatca cctccggcga cgtctgcggt
     5101 ccgaagagat gcgccacgtc gcggcgatag cggttggtga gatcgtaatc ggccagcacg
     5161 acctcgcgcg gcgcgcccac cgcgagcagc agcaccgccg cggcgaagcc ggtgcggtcc
     5221 ttgccgctcg tgcaatggaa aagcagcgga taattgtcag gctcggcggc gaaagcgagc
     5281 gcctggcggt attcttcgac atgctcgacg ccgaatttgc gatattggcc gagcacgagt
     5341 tgctccaatt ccaccgtgtc gatccgtccc aatcgcaccc gctcgagcat ttccagcgtg
     5401 ccggccggca ggaagccgag ctccagagtc cggatgcgcg cccccccggg caggcggttg
     5461 ggcttcaggc ggcgctccaa atcggtgcgg aaatcgacga gccctttgag gccgagcgca
     5521 tcgagccgcg ccaagtcggc gcgggtcagg tcggcgaggc tgtcggctcg gaacaagcga
     5581 cgccagcgcg tgcgccggcc gcccgcggcc ggatagccgc cgagatcgcg aaaattgatc
     5641 gcgccttcga aatcgagacg gcgggcgtag gcttcgctca taaccgggcg atgacgcggc
     5701 cggcgggcag cgtgatgtcg tattccatat agaccgcctt gacgatgcgc cgcaccggaa
     5761 agcggtcgac cggatcgaaa atcgagcgat ccggaaagga gatcgaggcc gggccttccc
     5821 aggcttcgcg caaatggtgc tgcgaatcgg cggcgacgag ttcggcgagc gccggccgcg
     5881 tcgtcgccgg atctgcggaa gggatgatcc ggagtccggt cgtcgggccg ttcgagccga
     5941 gttgcgagac cggctgttcg ggccgcatca cgacggtggc gagcctgacg cccgcgggac
     6001 gctccagctt gccgacgatc atgtcgcgat cgcgcgcaaa ttcgatacgc gcgagcttct
     6061 tcggaaagcc ccagacctcg cggccggcga gaagcggcgg ctcggtatcg acgaaaatct
     6121 gctggatgta ggtgagcggc ttgccgtcat gccgcgcata aagcctgaga atggcttcgt
     6181 ggtacggacc gaacgtgctg aacgggtacc gatagaccga gagattggcg caagccggct
     6241 cgatcagctc cagaggctcg ggcaaaatgt cgagggcggc gtcccggtcg gtctcgaatt
     6301 tgatcgagac cgcctgcgcg ttgcgatagt gatagggcgg cgcctggtac gccggcgcgt
     6361 cagccggcat ggaatagccg agccgttcga gcgtgagccg tcccctgccg ctcaaaacgc
     6421 cctccttcac gcggtcacgt actgaccgcc attgacgaag atggtctgac cctggatcat
     6481 cgaggcctct ggactggcga ggaactcgat caggccgaga taatcgtcat gcgtaatgtt
     6541 gcggccgctc ggattgccgg ccgcttcgct ggcgagaaac gcgtccgtcc cgtcgccgaa
     6601 cagattgcgc accgcctcgg tgtccagcgt tcccggcgcg acggcgttga tgcgaacgcc
     6661 gagcggcgcg agctcggcga cgagatagcg catcagcgcc tccgcgagcg ccttgccggc
     6721 gccgatcgcg ccatagccgg gaacgacctg ccggctgccg cggctggaga gaaagacaat
     6781 gcttgcgccg cgcttgagca gcggacgcgc cgcctggacg agaaagacga gcgacgcgcc
     6841 gttgagcgta agcgccgcgg ccaaggcgtc aggatcgacg tcgagcagcg ggccgaccag
     6901 caccttcacc gcgccatgga cgatgaggtc gagccgatcc gtcttcgccg cgacgtggtc
     6961 gatgagcagg cgcgcgccag ccggcgtcgc gacatccgcg cggatcggat gaggccgcgc
     7021 gccgagagcg gcgatctcgg ccgccgcttc cgcggcggcg gcctcgtctt gccgatagac
     7081 cataaaaatg tcagcgcgag gcttggcgaa gcgccgcgcg acggcgcggc cgaggccttt
     7141 cgcgccgccg gtgatgagaa ccgccattcc tcctcgccat ttgccgcagc ccctggagat
     7201 gtctacgtca tgcgacattg tatgacaata atgttgagca aaactgttga cgatctcggg
     7261 cgccgccgtt acgctttgcg ttaacaagct gcggcggacg ctaaaacgcc gcctggagga
     7321 gacgcagagg agcgagcgga cggtatccgg aggaaagctg cgcccctttg gcgcagcatg
     7381 tcggcgcccg acgacggcgc gccgccttcc ggccgtcttc tccctcgaaa agttcacgct
     7441 cgcagggagg gaagcatgca ggtgaaaacc ttgatggccg ccgcggccgc gcttttggcg
     7501 acgaccgctc tggccggttc agcggcggcg caggaaaagt ccaagaccgc gctgaaagtc
     7561 gcgatcttct cggtgaacgg cgcttcgccg accattcaca cgatgatcga atccgcgacc
     7621 gccgccgcca aggcgcgggg ctggacggtc gagacctatg acggcaacgg cgaccaggtc
     7681 gcgaccaaca atcaggccaa caccttcatc acgcgcggct tcgacgcgct gatcaacatc
     7741 gcctccgcca atacgcaaat gggcggcgtc atcgccaacg cggcgaaggc ccacattccc
     7801 tttgtctcca ctttttccgg catggtgccg ggcattacgg tcgatatcgg ctcgaacaat
     7861 gtcgccgacg gcgtcttttc cgcctccgct ttggccgccc gcatcgacgg cgaaggcgag
     7921 gtgctgaaga tgaactggac ggtgctgccc gccctcgccg agcgcgacgc cggcttccac
     7981 gccgttgtcg gcgaatacaa gaagctcaaa gtcaccgaga tggagatcaa ggtccctggc
     8041 caggtcgacg acacgttcaa caaggtcacc gactatctga ccggccacaa aaacgtgaag
     8101 gcgatctgga tgggctggga cgaggtcggc gtcgccgcgg ctcgcgccgt gcagcaatcc
     8161 catctcgagc acaagccctt cgtcgtcagc atggacggca acgacgccgc ctatgacatg
     8221 atccgcgagg gcacgccgct ctggctgacg atcgcctacg acgtgcgcgg catgggcgtg
     8281 caggcggtgc aagcggtcgc cgacgcggtc gacggcaagt ccttcccgca gcgccaaatc
     8341 tacaagaagc cgtgcctcat caccaaggag accgcgccgc cgaaaggcca atatcccgac
     8401 ttcaagactt gcgtccttta ttcagccgac ttgccgaagt cgaattaggt ttttcgccgc
     8461 tccaagagat catgtccgcg acgaaggctg cgcctgccct gtccgaaccg gatggggcgg
     8521 gccaagcaaa ggccgagata tttctcgaag tcgcgggact ggtgaaacag tttcccggca
     8581 cgcgggcgct cgaccgagtc gacttgacga tccgacgcgg cgagatccac gcgcttctcg
     8641 gcgagaacgg cgcgggcaaa tcgacgctga tccggcaaat ctgcggcgcc agccagccga
     8701 ccgaagggcg catcctgatc gagggcgcgg aggtctcgtt gccgtcgccc caggccgccg
     8761 ccgctttagg cattgcggtg gtgcatcagc acttcaacct cgtgccgcag ctttcggtct
     8821 gcgaaaatct gctgctctcc gaaagtctgc cgcggcgcgc cggcctcttc gtcgattggc
     8881 gagaggccta tcgccgcgcg cggacgctgc tgacgcgggt cgacctcgat ctcgatccac
     8941 gcatcgaggt ccgccggctc cggccggatg aagcggccat ggtggcggtc gccaaggcga
     9001 tcgcgacgaa cgccaagctg atcgttctcg acgagccgac aaccgccctg ctccccgccg
     9061 aagtctcggt tctgttcgcg catatgcgcc gcctcgccgg caaggggcat gccttcctct
     9121 atgtcagcca tcgcctggcc gaagtcttcg agatcgccga ccgcgtcacg gtgcttcgcg
     9181 acggccgcaa cgccggcgcc tggacacgcc aggagatgtc gcgccgtgcg atcatcgagg
     9241 ccattgtcgg cgcgcgaaag agctgggacg aggatgcggg gccggctccg gccgcactcg
     9301 gcgaagtcgt cctgcgcgcc gaagcgctgc gcggcggccg ggttgcggaa atgtccttcg
     9361 agttgcgcaa gcatgaaatc ctcggcgtcg ccggattgcc cggcagcggc gccgaggagg
     9421 cgctcgactt gctctatggc cgctatccga aggccggcgg gcgtctcagc gtcggcggcg
     9481 ccgacgtcga tttccgctcg ccgcgcgacg ccaaggcggc gggcctcgcc ttggcgccga
     9541 aggatcgcca tgccgagagc ctgctgcccg gcgccagcgt ccgggagaac atctcgctgc
     9601 cgaatctcgg ccaatacgtc gccgatccgg ttattcgctt tatccgccgc cgcacggagc
     9661 gccgcgacgc cgagcgcatc gccaagagcc tctcggtcaa gatgtcgagc atcgaggccc
     9721 cgatcgattc gctgagcggc ggcaaccagc aaaaggccgt gctcgggcga tggctcgcca
     9781 gcggcgcgaa gattttcatg ctcaattcgc caaccgcggc ggtcgatatc ggcgccaagg
     9841 cggagatcta cgcgctgatc cgccgcatcg ccgccgacgg cggagcggtg atcttcacct
     9901 cggcggaggt tgaggaattt ccacgcgtct gccggcgcgt gcttgtgttc cgcgacgggc
     9961 gcattgtcgg tgaactcgtc ggcaaggacg cgagcgaggc gaacattctg aaccttgcgg
    10021 ctggagccgg agatgagaac tagcgccaag gcgacggcgg ccgtcgcctc atcgagaacg
    10081 gatttcgcgc tgtggttcgc ccgctacggt acgctggtct cgctcgtcgc gctgctcgtc
    10141 gtcttctcga tcgcgcgccc ggacgttttt ccgacggtcg acaatctgct caacatcatg
    10201 aaccagattt cgattctagg cacgatggcc ttcggcctga cggtctgcct ggtcatgggc
    10261 ctgttcgacc tctcgatcgc ggcgatggcg acgctcggcg gctatgtcgc gacgttcctt
    10321 ctcgtgcaat atccggacgc gatcggcgtg ccggaggcgg tgttcatttc gctcgccatc
    10381 gccgcggcga tcggcgtcgt caatgggctc atcgtcagct atctcggcat ttccgccttc
    10441 atcgcgacgc tggcgaccgg ctcgatcatc accggcgcga tgctcggcat ttccgattcg
    10501 aagacgatca tcaccggcat cccggacgcg ttcatggcga tcggccaggg cgcgatcctc
    10561 ggcgtctcca atccgatcct gatcatgctc gcggtcggct tggcgctgtg gcttctgctc
    10621 gaacatacgc aagtcggccg gcatctctat gcgatcggcg gcagcgccga agcctcgcgc
    10681 ctgtccggcc tcgcggtgaa gcgctacgcg ccgttcgcgc tggcgatttg cgccgcctgc
    10741 gccgggctcg gcgggctgat ggcggcggcg gtgctcggcg cgggacgtcc gcaaggggtc
    10801 ggcgacggct atctgctcaa cgccttcgcc gccgtcttca tcggcgcctc gtcgctacgg
    10861 cccggcaaat tccatatcct cggcacgctg atcggcgtgc tgctgatcgg cgtgatcaat
    10921 aacggcctct ccatcatggg cgtgccgacc tattggcaat atatcgtgca aggcgtcctg
    10981 ctcgtcgcgg cgctgttcag cgccggcgtg ctgaccatgc gccggcattg acgctttcgc
    11041 gacggacttt tcaagcgcaa gcaggctcgc ccgatgcggc aaggcctcgg cgcagtcgcc
    11101 gcgacgccgg atttggcctg aatcgagccg cgccgccagg cgcgcgccga caatgcgagc
    11161 caacgccgga acttatcgtc tccggcggcg tttcgagtcg tctttcaatc attatccgta
    11221 ccaaagcgta agcggcgcgc gttccgatgg cgccgaccgc gagggagacg gcattgcgca
    11281 tcgctcagat ttcgcccttg accgaagccg tgccgccgaa actctatggc ggcaccgaac
    11341 gcgtgatttc atggctgacc gaggagcttg tcgcgctcgg ccatgatgtg acgctctttg
    11401 cgagcggcga ttccacgacg gcggcgcggc ttgagccgat gtggccgagg gcgctgcggc
    11461 tcgacggctc ggtgcgcgat cccaacgccc ttcatatgac catgatcgaa aaggtctgcc
    11521 ggcgggcggg ggaattcgac ttattgcatt tccatctcga ttattacccg ttttcggcaa
    11581 tgtcccgcca gccgacgcct tttctgacca ccctgcatgg ccgcctcgat ctgcccgagc
    11641 acgagccggt tttctcgacc ttctcggcaa ttcccgtcgt gtcgatttcc cacgcgcagc
    11701 ggcggcccgc gctgcaagcg cgctgggccg gcacggttca tcacggaatg ccggagacgc
    11761 ttctcaaacc cgaaccggcg aaagcgtcct atcttgcttt tctgggccgc atttcgccgg
    11821 aaaaacgggt cgaccgcgcc gtccatatcg ccgagcgttg cggcctgccg ctcaaggtcg
    11881 cggcgaaagt cgatcccgta gatcaggatt atttcaacga gaacatccgt ccgctcttcg
    11941 ccaagccgca tgtcgatttc atcggcgaaa tcgccgacgc gcagaaatcc gaattcctga
    12001 gcggcgccat cgccttgatc gtgccgatcg actggccgga gccgttcggg ctggtcatga
    12061 tcgaagcgat ggcctgcggc gcgccggtca tcgccttcaa tcgcggcgca gcgccggaac
    12121 tggtcgagga cggcgtgacc ggctttgtcg tcgaggacga gatcagcgcg gtcggcgccg
    12181 tccatcagct cgcccgcttg tcgcgcgaga ccatcaggaa gcgcttcgag gagcgcttca
    12241 ccgcccgccg catggcccgg gaatatttga aaatctaccg cgagttgctc ggcgcggcga
    12301 cgcagcggcc gaggatcgcc tcggtttccg gttaagcttc gccgccgccg gcgccggagc
    12361 gtgtttccgg catcgcgaac cgcgccgccg tcaagccgag caggccgatc gcggccatcg
    12421 ccaggaacgc cgccggcccg ccgagtcggt cgctgacata gccggcggcc gttgtgctga
    12481 tcgccgcgcc gacgcctacg gcgcagccga cggcgccctg ggcgagattg aaatgacccg
    12541 tatcgccggc gagatccgca acgatcagcg gcgccaggac gccgagcacc gcgccgcaga
    12601 cgccgtcaag caattgcacg gccatcagca gagtgggatt ggccgagtac gagaagaaca
    12661 aagcgcggat cgtcaaagcc gcgaaacaca ggacgagcaa gggccgccgt ccccattgct
    12721 cggcgcgccg gccgacccaa ggcgagagaa gcgcgacgac aagctgtggc ccgaggatcg
    12781 cgacggcgac caacatggcg gcgtccttgc tcgagcgcag cgtcattaag cccgccgcta
    12841 ggggcagcat ggcggcgttg gcgagctgga acagaacgat acagccggcg aagatcag
//
LOCUS       DATGJW010000426        11108 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_811010, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000426 DATGJW010000000
VERSION     DATGJW010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11108)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11108)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11108
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811010"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..210
                     /locus_tag="VKU03_12775"
     CDS             <1..210
                     /locus_tag="VKU03_12775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72187.1"
                     /translation="NPRRLPKRQVDKSRFVQRIKICAIHPYRESRRPTPRKLKADKAL
                     NRYPVFDGGGSLPRFVAARRRRSIA"
     gene            541..2595
                     /locus_tag="VKU03_12780"
     CDS             541..2595
                     /locus_tag="VKU03_12780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional aldolase/short-chain dehydrogenase"
                     /protein_id="HLJ72188.1"
                     /translation="MKNLWSDADAETMVATYAKKGVSRDLALRAYTTRLLGGEPRLVL
                     HGGGNTSCKIKVKDLVGDEWDALCVKGSGWDMAVIEPAGLPAVKLKPLLKARALDRLS
                     DENMVALQRANLIDPSSPNPSVETLLHAFLPHKFVDHTHSTAILSIVDQEDSRALCDK
                     IFGAKLGFVPYVMPGFALAKAAADIFDKDPTVEGLILDKHGIFTFGDDARQSYDRMIR
                     YVSMAEDYVAKNGKPAAKAAAAAARLAEPGEIAPILRGAVAFDRGEGRYDRFVVDFRS
                     TDKIREFLSYRDLDRIAETGVSTPDLVIRIKNKPLVLDPPAAGDLANYSRRVRERVAA
                     FVADYKAYFAKHDGSGGVKRIMLDPMPRLALVPGLGFFGFGRTLKDARIACDVAEMWV
                     EAAAGAEAIGRFHPVSMADLFDIEYWSLEQAKLAGAKPKPLTGQVALITGGAGAIGAA
                     TAKLFAANGAHVVVADLDGERAKEIAKAAGNGAIGTACDVTDPVSVRAAFDAAVATFG
                     GLDIAISNAGAAYEGAIGELDDALLRKSFEINFFAHQRVAQNAVRLFKLQQTGGALLF
                     NTSKQAVNPGANFGAYGLPKAATLFLSRQYALEYGKIGVRSNAVNADRIRSGILTEEM
                     IKSRSAARGVSEKDYMSGNLLGREVMAEHVAEAFLHHALAERTTGDVTTVDGGNIAAA
                     LR"
     gene            2689..3780
                     /locus_tag="VKU03_12785"
     CDS             2689..3780
                     /locus_tag="VKU03_12785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase dimerization/phosphoacceptor
                     domain -containing protein"
                     /protein_id="HLJ72189.1"
                     /translation="MAEEDDKAEQAKALVNTPNLADALESEQFRRFLDQVPIAIIVSE
                     IRDAERIVYANPEFEKVSGQIASALVGKRWSVLPGRRDGADAAAQLGEAITSKSDFVG
                     TFTIDRPGQDPVVADAYSNVIEDEAGTPCYRLAALVDVGAHSREQREEFEHRIREKDM
                     LLMEVQHRVKNNLQMITALIRIEARNARDRMDAAPFDRLAGRIQSIQLLYELLSGHGH
                     GDEIDLGVYLSNIASSVLRSHAVEGIRLDLKVDAYPVSLNVALPTGLVVNELLTNALK
                     HAFVGRDGGTITLNSLSDGNGCRIIVSDDGVGLREGVEWPKRGKLSALIVQSLQENAK
                     ARLDVASGPGKGMRVTISFPLSAAAPTAD"
     gene            complement(3836..4102)
                     /locus_tag="VKU03_12790"
     CDS             complement(3836..4102)
                     /locus_tag="VKU03_12790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72190.1"
                     /translation="MLHNLTAPLLAFAASFCLLAAAHAQTPAAPAEPFAGVQLAFNGC
                     GHNYYRAPDGRCDIVRNPNRDCPRGFHSVPAPTPSGYRCVQNGY"
     gene            complement(4352..4543)
                     /locus_tag="VKU03_12795"
     CDS             complement(4352..4543)
                     /locus_tag="VKU03_12795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72191.1"
                     /translation="MPSNIFRFDESWETPGASVEEVYDVLSHGELLPLWRKGVYLDAQ
                     RISGEGEPKVGDQFRVKAR"
     gene            complement(4591..5874)
                     /locus_tag="VKU03_12800"
     CDS             complement(4591..5874)
                     /locus_tag="VKU03_12800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094244.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72192.1"
                     /translation="MSAAARDGAAQEETSVRRIVLASLVGTTIEFYDFYIYGTAAALV
                     IGQTFFPDSAPQSQALKAFLTFGIAFLARPVGSVLFGHFGDRIGRKSTLVASMLVMGL
                     STTLIGILPGYAQAGAIAPWLLCLLRFGQGIGLGGECGGAALLAVENAPEGRRAWFGM
                     FPQLGPPLGFLLANGLFLLLLLGLGQQAFVAWAWRIPFLASAVLVAIGLYVRVTLSET
                     PAFQQAMARQERAKVPLLDVVRRHPVALLQGALAIVVCYALFYISTVFALSYGVNQRH
                     MASRDFLGLLCIAVLFMAAATPLAAFLADRFGRRPVLLAAQALAALTGFLLAPLLGGG
                     GTLSVLLFLALALGVMGLTFAPLGALLPELFPTRVGYTGASTAYNLGGILGASLAPSL
                     AQWLLQHGGLPGVGYYIVAAAAISFLSVLSMKETK"
     gene            6052..7722
                     /gene="betA"
                     /locus_tag="VKU03_12805"
     CDS             6052..7722
                     /gene="betA"
                     /locus_tag="VKU03_12805"
                     /EC_number="1.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019963327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008812 - choline dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0019285 - glycine betaine biosynthetic
                     process from choline [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="choline dehydrogenase"
                     /protein_id="HLJ72193.1"
                     /translation="MTESGETTFDYVIVGAGSAGCVLADRLSEDGRAEVLVVEFGGSD
                     RSPLIQMPAALSIPMNMEKYDWRFRSEPEPHLDNRRFHVPRGKVLGGSSSINGMVYVR
                     GNPFDFDGWEEEGAAGWAYRDVLPYFRRAESRRDGGDSYRGGDGPLATAYGRAENPLY
                     KVFIEAARQAGYDATSDINGEKQEGFGRFDMTVKNGVRWSAANAYLRPAMKRRNLHVV
                     TNARATKILFEGRRATGVRYLSGGGDRIAKARREVIVSSGPINSPILLKLSGVGPAQE
                     LRALGIPVIHDLPGVGENLQDHLEFYFQVASKQPITLYGRTGLFWRGLVGAHWLLRKK
                     GLGATNHFEAGGFIRSRAGVRYPDIQYHFLPLAVSYDGRGLASEHGFQAHVGPLRSKS
                     RGWVRLRSPDPREKPRIQFNYMSHEDDWIEMRACVRLTREIFAQSAFDPYRGREIRPG
                     ADVVSDEAIDAFVKRDVESAYHPSCTCRMGAKDDRNAVVDPEARVIGVEGLRVVDSSI
                     MPSITSGNLNAPTIMIGEKAADMILGRPPLPASNAPYYEAPNWRTAQR"
     gene            complement(7837..8718)
                     /locus_tag="VKU03_12810"
     CDS             complement(7837..8718)
                     /locus_tag="VKU03_12810"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ72194.1"
                     /translation="MNRPNYRLGLALVTASAVAWSTAGYFTRLIPLDAFTLLAWRGLF
                     GGVGIAVAGAALRRPAGFSLAIGRAGWLFAAVSALAMILFIVSLTLTTVAHAAVIYAT
                     VPFVAAGLGWLILRERPSGSALAASAAAFAGVAIMAGLSAEGRWLGDAIAFAMTLCMA
                     AIMVIARRFPNIPSLPAAALSAIISALVCWPLGHPLAVSGRELVLLALFGLANSALGI
                     GLFALGARYLPAVETALIGSLDAPLAPLWVWLAFSETPSAATIGGGLVVFAAVGLYLA
                     LGARISSPAKRGRGTAR"
     gene            complement(8830..9057)
                     /locus_tag="VKU03_12815"
     CDS             complement(8830..9057)
                     /locus_tag="VKU03_12815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72195.1"
                     /translation="MPEHHRPPDRAGDYRRHARGCIEAALHASDENACAAFLELAFSW
                     TRLAQRLEASERDAGDDFGAVQVVIIKPRRH"
     gene            complement(9146..10078)
                     /locus_tag="VKU03_12820"
     CDS             complement(9146..10078)
                     /locus_tag="VKU03_12820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlxA family transcriptional regulator"
                     /protein_id="HLJ72196.1"
                     /translation="MLVRHFSMIAFTAAIEPLRLANRESGKKLYEWRLYSVDGAAVEA
                     SNGVKVGVDHAFAEARNVESVVVCAGTDVQAIDHRALIATLRRLTKFGAAAGAVCTGA
                     YVLAKAGLLSGYRATIHWENLPGMRADFPDLNFGEDLFEIDRDRFTCAGGTAAADMML
                     SLISRDHGQRLSAAVTEQLIHHRIREAGERQRTDLRTRLGAAPPKLLRVVSLMEQSIE
                     RPLPVNEFAASVALSDRQLKRLFWKHLGRTPTRHYLTIRLEHACQLLRQTAMPILAVA
                     MASGFNSASHFSKSYYEHFGRSPSAERKACALAP"
     gene            10385..>11108
                     /locus_tag="VKU03_12825"
     CDS             10385..>11108
                     /locus_tag="VKU03_12825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007631748.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLJ72197.1"
                     /translation="MRRFSFAALLAEAANAHQGWGRQWRNPEPKKAYDIVIVGAGGHG
                     LATAYYLASVHGIANVAVLEKGWLGGGNTGRNTTIIRSNYLYDESAAIYEHAVKLWEG
                     LSQELNYNVMFSQRGVMMLAHNQHDEQSLKRHIYANRLNGVDNEWLTPEQAKAFCPPL
                     NMSRGIRYPVIGAALQRRGGVARHDAVAWGYARAADRLGVDIIQNCEVIGLTRGPSGA
                     IAGVETTRGSIGARRVGVVAAGH"
BASE COUNT         2050 a         3529 c         3748 g         1781 t
ORIGIN      
        1 aatccgcgcc gcctgccgaa gcgccaagtc gacaagagcc gctttgtcca gcgaatcaag
       61 atatgcgcga ttcatcctta tcgtgaatca cgccgcccca cgccgcgcaa gcttaaagcc
      121 gataaggcgc taaacagata ccccgtcttc gacgggggag gatcgttgcc ccgcttcgtc
      181 gctgcccggc ggcgtcggtc tatagcgtga tgaagtaggg gccggggcga cttatcctcc
      241 cccgttccgc gaagcggaac gggggagggg gaccatgcga agcatggtgg agggggcctg
      301 cggcgttggg atttatcctg atagactgga gccccctcca ccgcgctgac gcgcggtccc
      361 cctcccccgt cttcgacggg ggcggatagc tcctcagcaa tgtacttccg tgctgaccgc
      421 gctcggccgg gggctgatcc ccgcggcttt ccgccccgct tcctcgcgcc ctctttgcct
      481 cgcgcccgca tcgcgctaag cctccgcttc gaagcccggc aagcgccggc gggaggagac
      541 atgaagaacc tttggagcga cgccgacgcg gagacgatgg tcgcgactta cgccaagaaa
      601 ggcgtatcgc gcgatctcgc cttgcgcgcc tatacgacgc ggcttctggg cggcgagccg
      661 cgcctggttc tgcatggcgg cggcaacacg tcgtgcaaga tcaaggtcaa ggacctcgtc
      721 ggcgacgaat gggacgcgct ctgcgtgaag ggcagcggct gggacatggc ggtgatcgag
      781 cccgccggcc tgcccgcggt caagctgaag ccgctgttga aggcgcgcgc gctggaccgc
      841 ttatccgatg agaacatggt ggcgctgcag cgcgccaacc tgatcgatcc gtcgtcgccc
      901 aatccgtccg tcgagacttt gttgcacgcc tttctgccgc acaaattcgt cgaccacacc
      961 cattcgacgg cgattctgtc gatcgtcgac caggaagaca gccgggcgct gtgcgacaag
     1021 attttcggcg ccaagctcgg cttcgttccc tatgtcatgc cgggcttcgc gctggccaag
     1081 gcggcggccg atattttcga caaagacccc acggtcgagg ggctgattct cgacaagcac
     1141 ggcattttca ccttcggcga cgatgcgcgg caatcctacg accgcatgat ccgctacgtc
     1201 tcgatggcgg aggactatgt cgcgaagaac ggcaagccgg cggcgaaggc ggcggccgcg
     1261 gcggcgaggc tcgccgagcc cggcgagatc gcgccgatcc tgcgcggcgc ggtcgcgttc
     1321 gatcgcggcg agggacggta cgaccggttc gtcgtcgact tccgcagcac ggacaagatc
     1381 agggagttcc tctcctatcg cgacctcgat cgtatcgccg agaccggcgt ctcgacgccc
     1441 gacctcgtca tccggatcaa gaacaagccg ctcgttctcg atccgcccgc ggccggcgat
     1501 cttgcaaact attcgcggcg cgtccgcgag cgcgtcgccg ccttcgttgc cgactacaaa
     1561 gcctattttg ccaagcatga cggaagcggc ggcgtcaagc gcatcatgct cgatccgatg
     1621 ccgcggctgg ccctggttcc gggactcggc tttttcggat tcggccgcac gctgaaagac
     1681 gcgcggatcg cctgcgacgt cgccgaaatg tgggtcgagg cggcggcggg cgccgaggcg
     1741 atcggccgct ttcatcctgt cagcatggcc gaccttttcg acatcgaata ttggtcgctc
     1801 gaacaggcca agctcgccgg cgccaagccg aagccgctga ccgggcaagt cgctctcatt
     1861 accggcggcg ccggcgcgat cggcgcggcg accgccaagc tgttcgccgc caacggcgcg
     1921 catgtcgtcg tcgccgatct tgacggcgag cgggccaaag agatcgccaa agcggccggc
     1981 aatggcgcga tcggaacggc ttgcgacgtg accgaccctg tttcggtccg cgccgcgttc
     2041 gacgccgccg tcgccacctt cggcgggctc gacatcgcca tctccaatgc cggcgccgcc
     2101 tatgaaggcg cgatcggcga gctcgacgac gcgctgctga ggaagagctt cgagatcaat
     2161 ttcttcgccc atcagcgcgt cgcgcagaac gccgtccggc tgttcaagct gcagcagacc
     2221 ggcggcgcct tgctgttcaa cacgtcgaaa caggctgtca atccgggcgc caatttcggc
     2281 gcctacggcc tgccgaaagc cgcaacgctc ttcctcagcc ggcaatatgc gctggaatac
     2341 ggcaagatcg gcgtgcgctc caacgccgtc aacgccgacc gcatccgctc cggcattttg
     2401 acagaggaga tgatcaagag ccgctccgcc gcgcgcggcg tatcggagaa agattacatg
     2461 tccggcaacc tgcttggtcg cgaagtcatg gccgagcatg tcgccgaggc gttcctccat
     2521 catgcgctgg cggagcgtac gaccggcgac gtgacgacgg tcgatggcgg caatatcgcg
     2581 gcggcgttgc ggtagacgcg cgccgggccg gcagatcagg ccgtttgttc ggcctgcttt
     2641 gctcgatttc tccggttggc ctatacatgc gtcgcgagga gtttgaccat ggccgaagag
     2701 gacgacaagg ccgagcaggc caaggctctt gtcaacacgc ccaatctggc tgacgcgctc
     2761 gaaagcgagc agtttaggcg attcctcgat caagtgccga tcgcgatcat cgtatccgaa
     2821 ataagggacg ccgaacgcat cgtctatgca aatccggagt ttgagaaagt gtccggacaa
     2881 atcgcgtcgg cgttggtcgg aaagaggtgg agcgtccttc ccggccgccg cgatggcgcg
     2941 gatgcggccg cccaactggg cgaggcgatc acgagcaaaa gcgatttcgt cggcacattc
     3001 acgatcgatc gtccggggca agaccccgtg gtcgcggacg cctattcgaa cgtcatcgag
     3061 gacgaagcgg ggacgccctg ttatcggctc gcagcgcttg tggacgtagg ggcgcatagc
     3121 cgagagcagc gcgaagaatt tgagcatcga atccgcgaga aggacatgct tctcatggag
     3181 gttcagcatc gcgtcaaaaa caatttgcag atgatcaccg ccttgatccg catcgaggcg
     3241 aggaatgcgc gggaccgaat ggacgccgcc cccttcgatc ggctggccgg gcgcatacaa
     3301 tcgatacaat tgctctatga gctcttatcg ggtcatgggc acggagacga gatcgatctc
     3361 ggcgtctatc tcagcaatat agcgtcgtct gtcctgcggt cgcatgccgt ggagggcatc
     3421 cggctcgatc tcaaggtcga cgcctatccg gtgtcgttga atgtggcgct gccgaccggc
     3481 ctggtggtca atgagttgct gaccaacgcg cttaagcatg cgttcgtcgg ccgcgacggc
     3541 ggaacgatca cgttgaacag cctttcggac ggaaacggct gtcgcatcat cgtttcagat
     3601 gacggagtcg gcctacgcga gggcgtcgag tggccgaagc ggggcaagct cagcgcgttg
     3661 atcgttcagt ctctgcaaga gaacgcgaaa gctcgtcttg atgtcgcttc cggccccgga
     3721 aaaggcatgc gggtgacgat ttctttcccg ctttcggcgg ccgcgccgac ggcggactga
     3781 cgcggggatc accgcggaat ccgccgaggc gttcatctgg tcccgcccgt tgcgctcaat
     3841 acccgttctg cacgcagcga tagccggaag gcgtcggcgc gggcacgctg tggaagccgc
     3901 gcgggcaatc gcgattcggg ttcctgacga tgtcgcagcg gccatccggc gcgcgatagt
     3961 aattatggcc gcagccgttg aaggccagtt ggacgccggc gaaaggttcg gcgggcgcag
     4021 caggcgtttg ggcgtgagcc gcggcgagca ggcagaagct cgccgcgaag gcgagaagcg
     4081 gggcggtgag attgtggagc atggaccatc cttcgatttt tcgttcggaa cgataacgcc
     4141 tcggcgcggc gtcggtttcc ggcgcaaagc tcctcgcgcc tttacggtct ggcctggcgc
     4201 ctctccgcca gaaactcgct tttgccgcgc ggcgtggtcc agcggtgatt ccaggcggcc
     4261 ggccgaagca gcggcgccag ccggcgcagc atcggcctga gcacggtcac ttgccaaatg
     4321 atatcgacgt gaacgccgcg atcccgaggg ctcagcgcgc cttgacgcgg aattgatcgc
     4381 cgactttcgg ctcgccttcg ccgcttatcc tttgggcgtc gagatagacg cccttccgcc
     4441 agagcggcag caattcgcca tgcgacagga catcgtaaac ctcttcgacg ctcgcgccgg
     4501 gcgtctccca gctctcgtcg aagcgaaaga tgtttgacgg catcgcgtcc tcccctcaaa
     4561 ggggccgcgc cgaccgcgtc gccggcgcgc ctatttcgtc tccttcatgc tcaatacgct
     4621 caaaaagctg atcgcggcgg cggcgacgat gtaatagccg acgcctggca agccgccgtg
     4681 ctggagcagc cattgcgcca aactcggcgc gagcgacgcg ccgaggatgc cgccgagatt
     4741 gtacgcggtc gacgcgccgg tatagccgac gcgggtcggg aagagctccg gcaacagggc
     4801 gccaagcggc gcgaaagtca ggcccatgac gccgagggcc agcgccagga agagcagaac
     4861 cgacagcgtc ccgccgccgc cgagaagcgg cgccagaagg aaaccagtca gcgccgccag
     4921 agcctgcgcc gccagcaaga ccggacgacg gccaaagcgg tcggcgagaa aggccgcgag
     4981 cggcgtcgcc gccgccatga acaagaccgc gatgcagagc aggccgagaa aatcgcggct
     5041 cgccatgtgg cgctgattga cgccatagct cagcgcgaaa acggtcgaga tatagaacaa
     5101 ggcgtagcaa acgacgatcg ccagcgcgcc ttggagcagc gccacgggat ggcggcggac
     5161 gacatcgaga agcgggacct tggcgcgctc ctggcgcgcc atcgcctgct ggaaggccgg
     5221 cgtctccgag agcgtcacgc ggacataaag cccgatcgcc accaggacgg cgctggcgag
     5281 gaagggtatg cgccaagccc aggcgacgaa ggcctgctgg ccgaggccga gcagcagcag
     5341 gagaaaaagg ccgttggcga ggagaaagcc gagcggcggg ccaagctgcg ggaacatgcc
     5401 gaaccaggcg cgccgccctt caggcgcgtt ttcgacggcg agcagggccg cgccgccgca
     5461 ttcgccgcca aggccgattc cctggccgaa gcgcagcagg cacaagagcc agggcgcgat
     5521 cgcgccggct tgcgcatagc cgggcaggat tccgatcagc gtcgtcgaca ggcccatgac
     5581 cagcatcgac gcgaccagcg tcgatttgcg gccgatgcgg tcgccgaaat ggccgaacag
     5641 gaccgagccg accgggcgcg cgagaaaggc gatgccgaag gtcaggaagg ctttcagcgc
     5701 ctgcgattgc ggcgcagaat cggggaagaa cgtctggccg atcaccaaag cggcggccgt
     5761 gccgtaaata taaaagtcgt aaaattcgat cgtcgtgccg acgaggctcg ccagcacgat
     5821 ccgcctgacg ctcgtctcct cctgcgccgc gccgtcgcgc gccgccgcac tcatggccaa
     5881 aggcgttctc cccttatgcg ctccctctcg cgcttccgct attatcggcc aattgatgaa
     5941 atttccataa tcttgtttag tccatgatgg gcgcgcgccg cgcccgcgcc ggccgcttcg
     6001 gcgccgccca tcccgacatg agcaagcgac cggtttcgcg cggaggattt tttgactgaa
     6061 agcggcgaga cgactttcga ctacgtcatt gtcggcgcgg gctcggccgg ctgcgttctc
     6121 gccgaccggt tgtcggagga cggccgcgcg gaggttctgg tcgtggagtt cggcggctcg
     6181 gaccgctcgc cgctgatcca gatgccggcc gccctttcca ttccgatgaa tatggaaaag
     6241 tacgattggc gctttcgcag cgagcctgag ccgcatttgg acaatcggcg ctttcatgtt
     6301 ccccgcggca aggtcctcgg cggctcatcg tcgatcaacg gcatggtcta tgtgcgcggc
     6361 aacccgttcg atttcgacgg ctgggaggag gagggcgctg caggatgggc ttaccgcgac
     6421 gtgcttccct attttcgccg cgccgaaagc cggcgcgacg gcggcgattc ctatcgcggc
     6481 ggcgacggcc cgctggcgac cgcctatggc cgggcggaga atccgctcta caaggtcttc
     6541 atcgaagcgg cgcgccaagc cggctatgac gcaaccagcg acatcaacgg cgaaaaacag
     6601 gaaggcttcg gccgcttcga catgaccgtc aagaacggcg tgcgctggtc ggcggccaac
     6661 gcttatttgc ggccggcgat gaagcgccgg aacctacacg tcgtcaccaa cgcgcgggcg
     6721 acgaagattc tgttcgaagg ccgccgcgcg accggcgtgc gctatctcag cggcggcgga
     6781 gacaggatcg cgaaagcgcg ccgcgaagtg atcgtgagca gcgggccgat caactcgccg
     6841 attctcctca agctctccgg cgtcgggccg gcgcaggagc tgcgcgcgct cggcattccc
     6901 gtgatccatg acctgccggg ggtcggcgag aatctccagg accatctcga attctatttt
     6961 caggtcgcct ccaaacagcc gatcacgctc tatggcagga ccggcctgtt ctggcgcggc
     7021 ctggtcggcg cgcattggct gctgcgcaag aaggggctcg gcgcgaccaa ccatttcgag
     7081 gccggcggct tcatccgctc gcgcgccggc gttcgctatc ccgacattca gtaccatttt
     7141 ctgcccctgg cggtgagcta tgacggcagg ggtttggcga gcgagcacgg ctttcaggcg
     7201 catgtcggcc ccttgcgctc gaaaagccgc ggctgggtgc gcttgcgctc gcccgatccg
     7261 cgcgaaaagc cgcgcatcca gttcaactat atgagtcacg aggacgattg gatcgagatg
     7321 cgcgcctgcg tgcgcctgac gcgcgagatt ttcgcgcaaa gcgccttcga tccctatcgc
     7381 ggccgtgaga tcaggccggg cgccgatgtc gtcagcgacg aggcgatcga cgctttcgtc
     7441 aagcgcgacg tcgagagcgc gtaccatccc tcctgcacct gcaggatggg cgcgaaggac
     7501 gaccgaaacg cggtggtcga tccggaagcg cgggtgatcg gcgtcgaggg cctgcgcgtc
     7561 gtcgattcct cgatcatgcc gtcgatcacc tccggcaatc tcaacgcgcc gacgatcatg
     7621 attggcgaga aagcagcgga catgatcctc ggtcgcccgc ctctgccggc ctccaacgcg
     7681 ccgtactatg aagcgccgaa ctggcgaacc gcgcaaaggt agatatcctc ccccgtgaag
     7741 cgaagcggaa cgggggaggg ggaccggccg aaggccggtg gagggggcct gcggcggctg
     7801 ggattgtgct gatagaccgg aaccccctcc accgcgctat cgcgcggtcc ccctcccccg
     7861 ctttgcggga gaggagatgc gagcgccgag cgccaggtac agcccgacgg cggcgaagac
     7921 gacgaggccg ccgccgatcg tcgcggcgct cggcgtctcg ctgaaagcga gccagaccca
     7981 gagcggcgcc agcggcgcgt cgagcgaacc gatcagcgct gtctcgacag ccggcagata
     8041 gcgcgcgccg agcgcgaaca ggccgatgcc gagggcggaa ttggcgaggc cgaaaagcgc
     8101 gagcaggacg agttcgcggc cggaaacggc gaggggatga ccgaggggcc agcagacgag
     8161 cgcgctgatg atggcggaaa gcgccgccgc gggaagggac ggaatgttcg gaaatcggcg
     8221 cgctatgacc atgatcgcgg ccatgcagag ggtcatggcg aacgcgatgg cgtcgccgag
     8281 ccagcggccc tccgcgctga ggcccgccat gattgcgacg ccggcgaaag ccgcggcgct
     8341 ggcggcgagc gcggagccgc tgggccgctc gcgcaagatg agccagccaa gaccggcggc
     8401 gacaaaaggt acggtcgcat agatcaccgc cgcatgggcg acggtggtca gggtcagcga
     8461 gacgatgaac aagatcatcg ccagggccga aaccgccgca aacagccagc cggctcggcc
     8521 gatcgccaat gaaaatcccg ccggccggcg caaagcggcg ccggcgacgg cgatcccgac
     8581 gccgccgaaa aggccgcgcc aggcaagcag ggtaaaggcg tcgagcggga tgagccgggt
     8641 gaaatagccc gccgtgctcc aggcgacggc cgaggccgtc accaaagcaa ggccgaggcg
     8701 gtaattgggc cggttcatca ggctgatccg atcgcgacga tccgggacgt gaccgtctcg
     8761 cggcgacgat agccgcgcct gctgacacat tgtggcggga gaaagcgatt cgcaaacgat
     8821 cggaaacgat caatgccggc gcggcttgat gatcacgacc tggacggcgc cgaaatcgtc
     8881 gccggcgtct cgctccgacg cttccaggcg ctgcgccagg cgcgtccagc taaaggcgag
     8941 ttcgagaaaa gcggcgcagg cgttctcgtc cgaggcgtgg agcgccgcct cgatgcagcc
     9001 gcgcgcgtgg cgccgataat cgccggcccg atcaggaggg cgatgatgtt ccggcacaat
     9061 tcactcgtaa gcccccttcg gggcgtgaaa taaatatgac ggctggattg ttccggccgg
     9121 cgtcgaaatt tggattgacg ccggttcacg gcgccaaagc gcaggcctta cgctcggcgc
     9181 tgggactgcg gccgaaatgc tcataatagc ttttcgagaa atgcgaagcc gagttgaagc
     9241 cgctggccat ggcgacggcg agaatcggca tggccgtctg gcgcagcaat tggcaggcgt
     9301 gttcgaggcg gatggtaaga taatggcggg tcggcgtgcg gccgagatgc ttccagaaca
     9361 gccgtttgag ctggcgatcc gaaagagcga cgctggcggc gaattcgttg accggcagag
     9421 gccgctcgat cgattgctcc atcagcgaga cgacgcgcag cagcttgggc ggggcggcgc
     9481 cgagccttgt gcgcagatcg gttctctggc gctcgccggc ctcgcggatc cggtgatgga
     9541 tgagctgctc ggtaacggcg gcggaaaggc gctggccgtg atcgcggctg atcagcgaca
     9601 gcatcatgtc ggcggcggca gtgccgccgg cgcaggtgaa gcggtcgcgg tcgatctcga
     9661 acaagtcttc gccaaaattg aggtcgggga aatcggcgcg catgccgggc agattttccc
     9721 agtggatggt ggcgcgatag ccgctgagca ggccggcttt ggcgaggaca taggcgcccg
     9781 tgcacacggc gccggcggcg gcgccgaatt tggtcagccg ccgcagcgtc gcgatcagcg
     9841 cgcggtggtc gatcgcctgc acgtcggtgc cggcgcagac gacgaccgat tcgacgttgc
     9901 gcgcctccgc gaaagcatgg tcgacgccga ccttgacgcc gttcgaggcc tcgacggcgg
     9961 cgccgtcgac ggaatagagc cgccattcat agagcttctt gccggattcg cggttggcga
    10021 gccgcaacgg ctcgatcgcc gccgtgaagg cgatcatcga gaaatggcgg accagcatga
    10081 aggcgatgac atgcggcgcc gggcgctcgc gctgggcctc ctcgccgcgt aattcaatgc
    10141 gcgattttcc ttgcctgacg atcacctgaa gacgccttgc tccgcgccaa tcgctcgccc
    10201 gcatcaaagc agaatttgtc ggaaaatcaa aagatcgcgt cgcttgggct caagatggcg
    10261 cgggggcggg cggggagagt ccggaacccc ttctccccgc ttttgcgggg aggaggtggc
    10321 gcgaagcgcc ggatgagcgg ccaccctctc cccgctgcgc ggggcgacgg ggataggtcg
    10381 attcatgcgt cgtttttcat tcgcagcgct gctggccgag gccgcaaacg cccatcaggg
    10441 ctgggggcgg caatggcgca atcctgagcc gaagaaggcc tacgacatcg tcattgtcgg
    10501 cgccggcggc cacggcctcg ctacggccta ttacctcgcc agcgtccatg gaatcgccaa
    10561 tgtcgcggtg cttgaaaaag gctggctggg cggcggcaat accggccgca acacgaccat
    10621 catccgctcg aactatctct atgacgaaag cgccgcgatc tacgagcacg cggtcaagct
    10681 atgggaaggc ttgtcgcagg agctcaacta caacgtgatg ttctcgcagc gcggcgtgat
    10741 gatgctcgcg cataatcagc acgacgagca gagcctcaag cggcatatct acgccaaccg
    10801 cttgaacggc gtcgacaacg aatggctgac gccggagcag gcgaaggctt tctgcccgcc
    10861 gctcaatatg agccggggca tccgttatcc ggtgatcggc gcggccctgc agcggcgcgg
    10921 cggcgtcgcc cggcatgacg cggtcgcctg gggctatgct cgcgccgccg atcgcttagg
    10981 cgtcgacatc atccagaatt gcgaggtgat cgggctgacg cgcggcccga gcggcgcgat
    11041 tgccggcgtc gagacgacgc gcggctcgat cggcgcgcgc cgcgtcggcg tggtcgcggc
    11101 gggccata
//
LOCUS       DATGJW010000427         6010 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_704487, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000427 DATGJW010000000
VERSION     DATGJW010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6010)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6010)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6010
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_704487"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..2598
                     /gene="hrpB"
                     /locus_tag="VKU03_12830"
     CDS             133..2598
                     /gene="hrpB"
                     /locus_tag="VKU03_12830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018263258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent helicase HrpB"
                     /protein_id="HLJ72198.1"
                     /translation="MPSASDILPIDEILEQLRAALAAKSRVVLVAPPGAGKTTRTPLA
                     LLDQLFLHGRKLLLLEPRRLAARAAAQRMAATLGERVGETVGLRVRMEQLVSAKTRIE
                     VLTEGVFTRMILDDPSLEGIGAVLFDEFHERSLDADLGLALALDAQSGLREDLRLLVM
                     SATLDGARVASLLGEAPIVESAGRAYPVETRYVGRDPDLRIEEDAARIAARTLAAERG
                     SILVFLPGQGEIMRCAALLEERIRDPSVDIAPLFGAMDRAAQDKAIAPSPQGRRKIVL
                     ATSIAETSLTIEGVRIVIDSGLSRVPHYEPDIGVTRLETERVSRAGADQRRGRAGRTE
                     PGVCYRLWEEAANGALVPFARPEILSADLSGFVLDLAAWGVADPSRLAFLDPPPAPAL
                     DEARALLIAIGALGHSGRITDEGRAIARLALPPRLARMVVDAARQGQAALAARIAVLI
                     SERGLGGDGVDLAERLERWAKERSPRAEDARRLAGRIARGATDAAAPTEKESLSPGAL
                     LALAFPERIAKARGRRGEFLMANGRAASLSPETPLAGAPYLAIGEATGRAAATRILLA
                     AALEPDELEKLAADRIETKEETSFDRASASLRARRSRRLGAIVISEQTLSVAPSAENA
                     ALLAKSLLSLGIERLPWSKETKQWRDRVAFLARAEPDEWPDLSDAALSKPDWLVPFLT
                     GATSLADVTPALLAEALEAQLPYALRRRLDAEAPTHFTAPTGSRIPVDYEAEGGPAIA
                     LRVQELYGLKDHPTLANGKVPLTLHLLSPAHRPIQITRDLPGFWRGSWSAVKAEMRGR
                     YPRHIWPDDPAAAAPTTRAKPRG"
     gene            2668..3198
                     /locus_tag="VKU03_12835"
     CDS             2668..3198
                     /locus_tag="VKU03_12835"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003593636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HLJ72199.1"
                     /translation="MSDVAIVPIALDHVESYHRAVDVVARERKYLTILEAFPLPQTRE
                     FVLGLIKNGDPQFAALADGVVVGWCDIRRLPFPSQAHRGILGMGILPAFRGRGLGRRL
                     LTATLTQASRAGFVRIELDVRADNARAIALYEKAGFVREGVIRDAVCVDGVYCDAILM
                     ALVNRENAASSAKSRP"
     gene            complement(3182..4534)
                     /locus_tag="VKU03_12840"
     CDS             complement(3182..4534)
                     /locus_tag="VKU03_12840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLJ72200.1"
                     /translation="MAESRVLHRSLRGDLPIAARGEGVRLYDTRGREYLDACGGAAVS
                     CLGHSHPAPIAAIKEQAETLAYAHSAFFTSEPAERLAAELLKRAPKSFAKGRAMVLNS
                     GSEANEAALKLARQYHLEQGQSSRGKFIARESAYHGATIGALSAGGHRARRATYAPML
                     IDVGRIPACYAYRGRKEGESEEAYGQRVADALEREILRIGPENVAAFIAEPVSGATLG
                     AVPPVKDYFRRIREICDKYGALLIADEVMCGAGRTGTFLALEQEGVAADIVVIAKGLG
                     AGLQPIAAILASERVVKAIENGSGALAHGQTYMAHPMVCAAALAVLETIERDKLLDNV
                     RARGEQLGALLGARLGRRPHVGDIRGRGLFRGVEFVRDRESKTPFPTQAKLAQRLKAS
                     AMARGLMVYPASGCVDGISGDHVLLAPPYIVEPSDVELIVERFALAVDDALAGVRADF
                     "
     gene            4754..5605
                     /locus_tag="VKU03_12845"
     CDS             4754..5605
                     /locus_tag="VKU03_12845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLJ72201.1"
                     /translation="MMMILNRFLTLGGSLSVAIALLGLAQFGAPPAGASEANDAKLRV
                     FIDGPQRSPKNIARDRYRHPFAVLTFFGVRDSATIVEIDPGGAGYWTEILAPYLKDRG
                     RYIAANGPKDSPSEEVRRGNAAFAAKIAADSADYSKVEIAEFDPGRKDPSPPGQADFV
                     LTFRNIHNWMADGAAEAAFQAFYKALKPGGVLGVEEHRGRPDQPQDPKAKSGYVRQDY
                     AIHLAEAAGFKLAGESEVNANPKDTKDYPAGVWTLPPTFRLKDQDRAKYEAIGESDRF
                     VLKFVKP"
BASE COUNT          961 a         2080 c         1967 g         1002 t
ORIGIN      
        1 gatgtcccgc ctatggcggc gcccctatac aacgccgcct ctcccgctgg aagcctcgcc
       61 cgtcacgaag gattgcttaa ggaaaacgca tggctcttgc gtcggggcgg gcttcctgca
      121 aaggagccgg aaatgccctc cgcttccgat attctgccga tagacgagat tttagagcag
      181 cttcgcgccg ctcttgcggc aaaaagccgc gtcgttctcg tcgccccgcc gggggccggc
      241 aagacgaccc ggacgccgct ggcgcttctc gatcagcttt ttttgcatgg ccgcaaactc
      301 cttctgttgg agccgcggcg gctcgccgca cgggcggcgg cgcagcgcat ggcggcgacg
      361 cttggcgaaa gagtcggcga gacggtcggc ctcagggtgc gcatggagca actcgtctcg
      421 gccaagacgc ggatcgaagt cctgaccgag ggcgtgttca cccggatgat cctcgacgat
      481 ccgtcgctcg aaggaatcgg ggcggtgctg ttcgacgaat tccatgagcg ctcgctcgac
      541 gccgatctcg gcctcgcttt ggcgctcgat gcgcagagcg gcttgcgcga ggatttgcgt
      601 ctcctcgtca tgtcggcgac gcttgacggc gcccgcgtcg ccagcctgct cggcgaggcg
      661 ccgatcgtcg agagcgccgg gcgcgcctat ccggtcgaga cgcgctatgt cggccgagat
      721 cctgatttgc gcatcgaaga ggatgcggcc cgcatcgcgg cgcgcacgct cgccgccgag
      781 cgcggctcga tcctggtctt ccttcccggc caaggcgaga tcatgcgctg cgccgccctg
      841 ctcgaagagc gcattcgcga ccctagtgtc gatatcgcgc cgcttttcgg cgcgatggat
      901 cgcgccgcgc aagataaagc gatcgcgcct tcgccgcagg gccggcgcaa gatcgtgctc
      961 gcgacctcga tcgccgaaac ctcgttgacc atcgagggcg tgcgcatcgt catcgatagc
     1021 ggcctctcgc gcgtacccca ttacgagccc gatattggcg tgacgcggct ggagaccgag
     1081 cgcgtttcgc gcgccggcgc cgatcagcgc cgcggccggg ccggccgcac cgagccgggc
     1141 gtctgctatc ggctttggga ggaggcggcg aatggcgcgc tcgtcccctt cgcgcggccg
     1201 gaaatcctct ccgccgatct ttccggcttc gtcctcgacc ttgccgcctg gggcgtcgcc
     1261 gatccgtcgc gcctggcctt tcttgacccg ccgcccgccc ctgctctcga cgaggcccgc
     1321 gcgctgctca tcgcgatcgg cgcgctcggc cattctggcc gcatcaccga cgagggccgc
     1381 gccatcgcca ggctcgcttt gccgccgcgc ctggcgcgca tggtcgtcga cgcggcgcgg
     1441 caggggcaag ccgcgctcgc cgcgcgcatc gccgtgctca tttccgagcg aggccttggc
     1501 ggcgacggcg tcgatctcgc cgagcggctg gagcgctggg ccaaggagcg ctcgccgcgc
     1561 gccgaggacg cgcgccgcct cgccgggcga atcgcgcggg gcgcgacgga tgccgccgcg
     1621 ccgacggaga aggagagcct cagccccggc gccctgcttg cgctggcgtt tcccgagcgc
     1681 atcgccaagg cgcgcggccg gcgcggcgaa ttcctgatgg ccaatggccg cgcggcgagc
     1741 ttaagccccg agacgcccct cgccggggcg ccgtatcttg cgatcggcga agcgaccggc
     1801 cgcgccgccg ctacgcgcat tctcctggcc gccgcccttg agccggacga gttggagaag
     1861 ctcgcggcgg accggatcga gacgaaagag gagacgtcgt tcgaccgcgc ctcggcctcc
     1921 ctgcgcgctc gccgctcgcg ccggctcggc gcgattgtaa tatccgagca gacgctttcc
     1981 gtcgcgccga gcgccgaaaa cgccgccctt ctcgccaaga gtctcctgtc gctcggcatc
     2041 gagcgcctgc cttggagcaa agagacgaaa caatggcgcg accgcgtcgc ctttctcgcc
     2101 cgcgccgagc ccgacgaatg gcccgattta tccgacgccg ccttaagcaa gcccgattgg
     2161 ctcgtcccgt ttctgaccgg cgcgacgtcg ctcgccgatg tgacgccggc tctcctcgcc
     2221 gaagcgctcg aagcgcaatt gccctacgcg ctgcgccgcc ggctcgacgc cgaagcgccg
     2281 acccatttca ccgcgccgac cggctcgcgc attcccgtcg actatgaagc ggaaggcggg
     2341 cctgccatcg ctttgcgggt gcaggagctt tacggcctta aagaccatcc gaccctcgcc
     2401 aacggcaagg tcccgctgac gctgcatctt ctctcgccgg cgcatcggcc gatccaaatc
     2461 actcgcgacc tgccgggctt ctggcgcggc tcgtggagcg ccgtcaaagc cgagatgcgc
     2521 ggccgctatc cgcgccatat ctggccggac gatccggccg ccgccgcgcc gacgacaagg
     2581 gcgaagccga gaggatagcg aagggttgtc ggcgcgcagg cgatttgacg tatgctcatt
     2641 gctcaaaacc ggtaggagaa gaagcgcatg agcgatgtcg ccatcgttcc gatcgcgctc
     2701 gatcatgtcg agagctatca tcgcgcggtg gatgtcgtcg cccgcgaacg gaagtacctg
     2761 acgatcctcg aagcctttcc tttgccgcag acgcgcgaat tcgtcctcgg cctgatcaag
     2821 aacggcgatc cgcaattcgc ggccttggcc gacggcgtgg ttgtcggctg gtgcgatatt
     2881 cggcgcctgc cctttccgag ccaagcccat cgcgggattc tcggcatggg aatcctgccc
     2941 gcctttcgcg gccgcggcct tggccgccgg ttgctgaccg cgactttgac gcaggcgtcg
     3001 cgggcgggat tcgtgcggat cgaactcgac gtacgcgccg acaatgcccg cgccatcgcg
     3061 ctttacgaga aagccggatt cgtccgggag ggcgtcatcc gcgacgcggt ctgcgtcgac
     3121 ggcgtctatt gcgacgcgat cctgatggcg ctcgtcaatc gcgagaatgc ggcaagctcc
     3181 gctaaaagtc ggccctgacg ccggcgagcg catcgtccac ggccaaggcg aagcgctcga
     3241 cgatcagctc cacatcgctc ggttcgacga tataaggcgg cgccagcagc acgtggtcgc
     3301 ctgaaatccc gtcgacgcag ccgctcgccg gatagaccat caggccacgc gccatcgccg
     3361 aagctttcag cctttgcgcg agcttggctt gggtgggaaa cggcgtcttg ctttcccggt
     3421 cgcggacgaa ttcgacgccg cgaaacaagc cgcggccgcg tatgtcgccg acatgcgggc
     3481 gccgtccaag ccgggcaccg agcaacgcgc ccaattgctc gccacgggcg cggacattgt
     3541 cgagaagctt gtcgcgctcg atcgtctcca gcacggcgag cgcggcggcg cagaccatcg
     3601 gatgggccat ataggtttgg ccatgcgcca gcgcgcctga gccgttctcg atcgctttca
     3661 caacgcgctc gctggcgagg attgcggcga tcggctgcaa cccggcgccg agccccttgg
     3721 cgatgacgac gatgtccgcg gcgacgcctt cctgctccag cgccaagaag gttccggtgc
     3781 ggccggcgcc gcacataacc tcatcggcga tcagaagggc gccgtatttg tcgcaaatct
     3841 cgcggatgcg cctgaaatag tccttgaccg gcggaacggc gccgagcgtc gcgccggaaa
     3901 ccggctcggc gatgaaagcg gcgacgttct caggcccgat ccggagaatt tcccgctcca
     3961 gcgcgtcggc gacgcgctgg ccataggcct cctcgctttc gccctccttc cgaccgcgat
     4021 aggcgtagca ggcggggatg cggccgacat cgatcagcat cggcgcatag gtcgcgcgcc
     4081 gcgcccggtg gccgccggcc gaaagcgcgc cgatcgtcgc gccgtgatag gcggattcgc
     4141 gggcgatgaa tttgccgcgg ctgctttgcc cttgttcgag atgatattgc cgcgccagct
     4201 tcagcgcggc ctcattggct tccgagccgg agttcaagac catcgcgcgg cccttggcga
     4261 agctcttcgg cgcgcgcttg agcaattcgg ccgcaaggcg ctcggccggt tcgctggtga
     4321 agaacgcgct atgcgcgtag gcgagcgtct cggcctgctc cttgatcgcc gcgatcggcg
     4381 ccggatgcga atggccgagg caggagaccg ccgcgccgcc gcacgcatcg agatattcgc
     4441 ggccccgcgt atcgtagagg cgcacgccct ctccgcgcgc ggcgatgggc aaatcgcccc
     4501 tgagactgcg atggagcact cggctttcgg cgatcagcaa aggaaaactc tccccatatc
     4561 gtcattgcga ggcgcgaagc gaagcaaagc aaaccattca tagggcgctc tacgaatgga
     4621 tggcttcgct tcgctcgcaa tgacgaaatg cggcttgatg aaccggtcgc cgtcgcggcg
     4681 caagcgccgc gacggcgaca aatcgcattg acggcggggc cgctcacttt attatttcac
     4741 tgacgaaaga gctatgatga tgattctcaa caggtttttg accctcggcg gctctttgag
     4801 cgtggcgatc gcccttctcg gcctcgcgca attcggcgcg ccgccggccg gcgcctcgga
     4861 ggcgaacgac gccaagctgc gcgtcttcat cgacgggccg cagcgctcgc cgaagaatat
     4921 cgcgcgcgac cgctatcgcc atccgttcgc ggtcctgaca ttcttcggcg ttcgggacag
     4981 cgcgacgatc gtggaaatcg atcccggcgg cgccggctat tggacggaga ttctcgcgcc
     5041 gtaccttaag gaccgcggcc gctatatcgc ggcgaatggt cccaaggact cgccgtcgga
     5101 agaggtccgg cgcggcaacg cggctttcgc ggccaagatc gccgccgatt cggccgacta
     5161 ttccaaggtt gaaatcgccg aattcgatcc cggccgaaag gacccgtccc cgcccggtca
     5221 ggccgatttc gtccttacct tccgcaacat ccacaattgg atggcggacg gcgcggcgga
     5281 ggcggcgttc caggctttct acaaggcgtt gaagccgggc ggcgtgctcg gcgtcgagga
     5341 gcatcgcggc cgccccgatc agccgcaaga cccgaaggcg aagagcggct atgtgcggca
     5401 ggactacgcg atccatctcg ccgaagccgc cggcttcaag ctcgccggcg agtccgaggt
     5461 caacgccaat ccgaaggaca ccaaagatta tccagccggc gtctggaccc tgccgccgac
     5521 attccggctc aaggatcagg accgcgccaa atacgaagcg atcggcgaga gcgaccggtt
     5581 cgtactgaaa ttcgtgaagc cttaaccccc tcgccccgcg cagcggtagc cagtcgacca
     5641 acggcgcgca ctttcgagtt ctgcgcagcc cctcatccgg cgcttcgcgc tgcgttctcc
     5701 tcgcgaagcg ctacggcatg cacacatctt gaagcgcggc gcgcatcggc ccggcatcgt
     5761 cacccgaagg ggcgcagcga agcggcgcag cggccatagc ccgctgcgca gagcttggtc
     5821 ggttcgcgca acggtcgggt cgggctatgg ccgcttgacg atgccgggcc gatgtggccc
     5881 cgcgccgaga tgcgtgcatg ccgtagtcgc gaagcggaga gaagggcctc gtcgcctcac
     5941 ttcgcgacca gggcgtccct gatttccgtc agcagcttct cgcttgtggt cggcgccggc
     6001 ggagcggccg
//
LOCUS       DATGJW010000428         4612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_811307, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000428 DATGJW010000000
VERSION     DATGJW010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4612
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_811307"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="hflC"
                     /locus_tag="VKU03_12850"
     CDS             complement(<1..898)
                     /gene="hflC"
                     /locus_tag="VKU03_12850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008974516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protease modulator HflC"
                     /protein_id="HLJ72202.1"
                     /translation="MTRPASGFALMALVVVAIIIVHGALFTVDPTEQALVLRFGEPVR
                     DVIGAPGLYFKWPLIDSVIPIDKRILDLDAPRQEVLVSDNQRLEVDAFLRYRISDPLK
                     FYQSVGTIQGANDQLDGMLNSAVRRTLGEASIADIVREKRDALMGEIRDQIKAASERF
                     GIDIVDVRIRRANLPPENSEAVFRRMQTERQQRAATFRAQGSQQSQQIKAEADRKVTV
                     TVAQAQQQAEQIRGQGDAERNRIFAEAYGKDPDFFAFYRSMQAYENGLKASNSRIVLS
                     PKSDFFRYFTTPTPPPEPASAAP"
     gene            complement(895..1998)
                     /gene="hflK"
                     /locus_tag="VKU03_12855"
     CDS             complement(895..1998)
                     /gene="hflK"
                     /locus_tag="VKU03_12855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265890.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0045861 - negative regulation of
                     proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsH protease activity modulator HflK"
                     /protein_id="HLJ72203.1"
                     /translation="MSWSNQGGPWRSGQGPWGQGPSGPQPPGDLEEMLRRLQGFFRGL
                     TPGGGGSTARWTLFGGLIVVILWLLWGFYTVQPNEVGINLVLGRYTGKTAAGLNYNWP
                     APIGSVIKVPVWVQKITRIGLRGDEDNDMVAARSGAPEESLMLTSDLNIVDVKFRVIW
                     QIDPAKPEDFVFNIRRPEETVKAVAESVMREVVGLKTIDGILTTDRKVIEPDVQRRMQ
                     YVLDSYKAGVLIRQVQLQSVDAPAQVISAYRDVTAAQQDEQRMVNEAETYANRVVPEA
                     EGSAARIVLEAKAYREQTVADAKGQTSRFNQVYEQYKNAPAVTRERMYLETMERVLGG
                     MPKTILDVSGVAAPLPYLSLDQLQTNKPTGAAK"
     gene            complement(2073..3365)
                     /locus_tag="VKU03_12860"
     CDS             complement(2073..3365)
                     /locus_tag="VKU03_12860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyC/CorC family transporter"
                     /protein_id="HLJ72204.1"
                     /translation="MTFTIAALIVVASLIASAFFSGAETALTAASRVRMLALEKSGNK
                     RATSVAKLITQRGRLIGAMLLGGQLVTIAASSFTTNVLVSVVGDRGVVYATAIMTVLI
                     VVFAEVLPKTVAIAYPDRVSLVIAPLVSFFVAIFSPIVATIETFVRALLSLGGLRIGE
                     RASMLSGHEELMSAVELLHREGGVARDERDMFGGLIELSELEVSDVMIHRTNMRAIDA
                     DLPPMELVREVLASPYTRMPLWRGNPDNIIGVLHAKDLLRALAAAGGDAGKLKIDDIA
                     LEAWFVPDTTSVRDQLQAFLRRKTHFALVVDEYGVVLGLVTLEDILEEIVGDIRDEHD
                     VAIQGVRPQPDGSVIVDGSAPVRDLNRTMDWSLPDDEATTIAGLVIHEARTIPEPKQI
                     FTFHGFRFEVLRKNRNRITALRVAPASPPGNADGAPED"
     gene            complement(3392..>4612)
                     /gene="aroB"
                     /locus_tag="VKU03_12865"
     CDS             complement(3392..>4612)
                     /gene="aroB"
                     /locus_tag="VKU03_12865"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016921040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HLJ72205.1"
                     /translation="DGPHEIVVDAIIAALARRAPGQAGEPAPSGTDQAQRQRREVDVA
                     LGERAYKIVIGDGLIEEAGAHIARLAPDAHCAIVTDENVAPLHLDALRRSLAAAGLRE
                     TAILVPPGETSKSFSQFERVCDAILAAHIERRDIVIALGGGVIGDLAGFAAASVRRGL
                     RLVQMPTTLLAQVDSSVGGKTAINSRHGKNLVGAFHQPALVLADTRVLDTLGEREFRA
                     GYAETVKYGLIGDRPFFDWLEASWRDVFAGGPARLRAIATSCAAKAATVAADETEQGE
                     RALLNLGHTFGHAFERLVDYDGARLVHGEAVAIGMTCAFRFSRRLGLCPGQDAVRVEN
                     HLRAVGLPTEMRDIPRFAAEPDAILAAMRQDKKVERGRLTFILARGIGESFIAKDVAE
                     ADIRAFLADEFARA"
BASE COUNT          779 a         1477 c         1584 g          772 t
ORIGIN      
        1 ccggcgcggc cgacgccggc tcgggcggcg gcgtcggcgt cgtgaaatag cggaagaagt
       61 cggatttcgg gctcagcacg atgcgcgagt tcgaggcttt caagccgttc tcgtaggctt
      121 gcatcgagcg gtaaaacgcg aagaaatccg gatccttgcc ataggcttcg gcaaagatgc
      181 ggttgcgctc ggcgtcgcct tggccgcgaa tctgctcggc ttgctgctgc gcctgggcga
      241 ccgtcacggt gactttccgg tccgcctcgg ccttgatctg ctggctctgc tgcgagcctt
      301 gggcgcggaa ggtcgcggcg cgctgctggc gctcggtctg catgcggcgg aacaccgcct
      361 cggaattttc cggcggcagg ttcgcccttc tgatccgcac gtcgacaata tcgatgccga
      421 agcgctcgga ggccgccttg atctgatcgc gaatctcgcc catcagggcg tctcgcttct
      481 cgcgaacaat gtcggcgatg gaggcctcgc cgagcgtgcg gcgaacggcg gaattgagca
      541 tgccgtcgag ctgatcgttg gcgccctgga ttgtgccgac cgactgataa aattttagcg
      601 ggtcggagat gcggtagcgc aggaaggcgt ccacttcgag gcgctgattg tcggaaacca
      661 gcacttcctg cctcggcgcg tcgaggtcga gaatgcgctt gtcgatcggg atcaccgaat
      721 cgatgagcgg ccatttgaaa taaaggccgg gcgcgccgat cacgtcgcgc acgggctcgc
      781 cgaagcgtag gaccaaggcc tgttcggtcg gatcgacggt gaaaagcgcg ccgtgaacga
      841 tgatgatcgc gacgacgaca agggccataa gcgcaaagcc gctggctgga cgggtcattt
      901 cgccgctccc gtcggcttgt tggtctgaag ctgatcgagc gacaggtagg gcagaggcgc
      961 agcgacgccg gaaacgtcga ggatggtctt cggcatgccg cccaacacgc gctccatggt
     1021 ctcgagatac atgcgctcgc gcgtcaccgc cggcgcgttc ttgtactgct cgtaaacctg
     1081 gttgaaacgc gacgtctggc ccttggcgtc ggcgacggtc tgctcgcgat aggccttggc
     1141 ttcgagcacg atgcgcgcgg ccgagccttc cgcctccgga acgacccgat tggcgtaagt
     1201 ctcggcctcg ttgaccatgc gctgctcgtc ctgctgggcg gcggtcacgt cgcgataggc
     1261 ggagatgact tgcgccggcg cgtcgaccga ttgcaattgc acctgccgga tcagcacgcc
     1321 ggctttgtac gaatcgagca catattgcat gcgccgctgc acgtcgggct cgatcacctt
     1381 gcggtcggtg gtcaggatgc cgtcgatcgt cttcaggccg accacttcgc gcatcacgct
     1441 ttccgccacc gccttcaccg tttcttccgg ccggcggatg ttgaagacga agtcttccgg
     1501 cttggcgggg tcgatctgcc agatgacccg gaacttcacg tcgacgatgt tcaggtccga
     1561 ggtcagcatc aagctctctt cgggcgcgcc ggagcgcgcg gcgaccatgt cgttgtcctc
     1621 gtcgccgcgc aggccgatcc gggtgatctt ttgaacccag accgggacct tgatgaccga
     1681 gccgatcggc gccggccaat tgtagttgag gcccgccgcg gtcttgccgg tatagcggcc
     1741 gagcacgaga ttgatgccga cctcgttcgg ctggaccgtg tagaagcccc agagcagcca
     1801 cagaatgacc acgatcaggc cgccgaacag cgtccagcgc gcggtcgagc cgccgccgcc
     1861 cggcgtcagg ccgcggaaaa agccctgcag gcggcgcagc atctcttcga gatcgcccgg
     1921 cggctgcggg ccgcttggac cctgtcccca cggtccctgg ccggaacgcc atggaccgcc
     1981 ttgattgctc caggacatga accctcttcg acactgagcc ggctcggcgg gccgcttata
     2041 taggcgacaa gcgcttgtgc ggcaacgcag cgctaatctt ccggcgcgcc gtcggcgttc
     2101 cccggcggcg aagccggcgc aaccctcagc gctgtgatgc ggttgcggtt cttgcgcagc
     2161 acttcgaagc ggaagccgtg aaaggtgaag atctgcttgg gctcggggat cgtgcgcgcc
     2221 tcgtgaatga cgagcccggc gatcgtggtc gcctcgtcgt cgggcagcga ccaatccatg
     2281 gtccggttga gatcgcgcac cggcgccgag ccgtcgacga tgaccgagcc gtccggctgc
     2341 ggccgtacgc cctgaatcgc gacgtcgtgc tcgtcgcgga tgtcgccgac gatctcttcg
     2401 aggatgtctt cgagcgtgac caggccgagc acgacgccat attcgtcgac gaccagcgcg
     2461 aaatgggtct tgcggcgcag gaaggcctgc aattgatcgc gcaccgaggt cgtatccggc
     2521 acgaaccagg cttcgagcgc gatgtcgtcg atcttgagct tgccggcgtc gccgcccgcc
     2581 gccgcgaggg cgcggagcaa atccttggcg tgcagcacgc cgataatgtt gtccggattg
     2641 ccgcgccaga gcggcatgcg ggtatagggg gaggcgagga cctcgcgcac cagctccatc
     2701 ggcggcagat cggcgtcgat ggcgcgcata ttggtgcggt ggatcatcac gtcggagact
     2761 tcgagctcgg aaagctcgat caggccgccg aacatgtcgc gctcgtcgcg ggcgacgccg
     2821 ccttcgcggt gaaggagctc gacggcgctc atcagctctt catggccgga cagcatcgag
     2881 gcgcgctcgc cgatgcgcag gccgccgagc gacagaagcg cgcgcacgaa agtctcgatc
     2941 gtggcgacga tgggcgagaa aatcgccacg aaaaaggaga cgagcggcgc gatgaccagc
     3001 gagacgcggt cgggataggc gatggcgacg gtcttcggca gcacttcggc gaagacgacg
     3061 atcagcacgg tcatgatcgc ggtcgcgtaa acgacgccgc ggtcgccgac gacgctgacg
     3121 agaacgttgg tcgtgaagga cgaagcggcg atcgtcacga gctggccgcc gagcagcatc
     3181 gcgccgatca agcggccgcg ctgcgtgatc agcttggcga ccgaggtcgc gcgcttattg
     3241 cccgacttct ccaaagcgag catgcggacg cgcgaggcgg cggtcagcgc ggtctcggcc
     3301 ccggagaaga acgccgacgc gatcaggctg gcaacgacga tgagcgccgc aatggtgaaa
     3361 gtcatggctc cctcggatga ggcgacgatc cttaagcgcg cgcgaattcg tcggcgagga
     3421 aagcgcgaat atcggcctcg gcgacgtcct tggcgatgaa gctctcgccg atgccgcgcg
     3481 ccagaatgaa ggtcagccgg ccgcgctcga ctttcttgtc ctggcgcatc gcggcgagga
     3541 tcgcgtcggg ttctgcggca aagcgcggaa tgtcgcgcat ttcggtcggc aggccgacgg
     3601 cgcgcaaatg attttccacc cggaccgcgt cctgtcccgg acagaggccc agccggcgcg
     3661 agaaccgaaa ggcgcacgtc atgccgatcg ccaccgcttc gccatggacg agccgcgcgc
     3721 cgtcataatc gacaagccgt tcgaaagcgt ggccgaacgt atggccgaga ttgagcaagg
     3781 cgcgctcgcc ctgctcggtc tcgtcggcgg cgacggttgc ggccttggcc gcgcagctcg
     3841 tcgcgatggc gcgcaggcgg gccgggccgc cggcgaagac gtcgcgccag ctcgcttcga
     3901 gccagtcgaa gaacggccga tcgccgatca ggccgtattt gaccgtctcg gcatagccgg
     3961 cgcgaaattc gcgctcgccc agcgtgtcga ggacgcgcgt gtcggcaagg accagcgccg
     4021 gttgatgaaa ggcgccgacg aggttcttgc cgtggcggga attgatcgcg gtcttgccgc
     4081 cgaccgacga atcgacttgc gccagcaagg tcgtcggcat ttgcacgagg cggaggccgc
     4141 gccggacgct cgccgcggcg aagccggcga gatcgccgat cacgccgccg cccagggcga
     4201 tcacgatgtc gcgccgctcg atatgggcgg cgaggatcgc gtcgcagact cgctcgaatt
     4261 gcgaaaagga tttcgacgtt tcgccgggcg ggacgaggat cgccgtctcg cgcaaaccgg
     4321 ccgcggcgag gctccgccgc aacgcgtcga gatggagcgg cgcgacattc tcgtcggtga
     4381 cgatcgcgca atgcgcgtcg ggcgcgagcc gcgcgatatg cgcgccggcc tcctcgatca
     4441 atccgtcgcc gatgacgatc ttgtaggcgc gctcgccgag cgcgacatcg acctcgcggc
     4501 gctggcgctg cgcctgatcc gtcccgctcg gcgccggttc gccggcttgg cccggggcgc
     4561 ggcgcgccag cgccgcaata atcgcgtcga ccacaatctc gtgcgggcca tc
//
LOCUS       DATGJW010000429         3178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_291854, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000429 DATGJW010000000
VERSION     DATGJW010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3178
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_291854"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(56..853)
                     /locus_tag="VKU03_12870"
     CDS             complement(56..853)
                     /locus_tag="VKU03_12870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72206.1"
                     /translation="MESKPAEGKPAQPPLIEGIDLVREFGGLSAVDGMSIALRRGEIL
                     GLIGPNGAGKTTLFNLLAGSLRPSAGAVLIAGRDVSAAGPESRLKLGVGRTFQIPRPF
                     AAMTVLENVLTGGQRQAGERIAMNFLRPGRVAAEERRAVEKARALIDFVSLSRLENEP
                     ARVLSGGQRKLLELARILMADPQAILLDEPAAGVNPALLELIIDRIRALNAEGKTIML
                     IEHNMAMVSRLCGRVVAMASGRRLAEGAPAEVAADRKVIAAYLGEAA"
     gene            902..1273
                     /locus_tag="VKU03_12875"
     CDS             902..1273
                     /locus_tag="VKU03_12875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004115415.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04076 family protein"
                     /protein_id="HLJ72207.1"
                     /translation="MTTREADKGAAADDAFDLYDLRVEVVAPEGGAIFCGARPGDHFE
                     LRGEMLFLPPGQGFSIYSLAALLPLLPAKQRPTHPNDWMTSDAEIACPDPNCATRFRI
                     RRLGLRRFRHSETTATPLGGT"
     gene            1280..2329
                     /locus_tag="VKU03_12880"
     CDS             1280..2329
                     /locus_tag="VKU03_12880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006728273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ72208.1"
                     /translation="MERFRLAPDYEISRVIRGGWQMAAGHGAVRASDPIADLIAFADA
                     GVTTFDCADIYTGVEELIGNFRLRYRALRGEEAAGRIRVHTKFVPDLDFLPRIQKSDV
                     ERVIDRSLRRLNMERLDLVQFHWWDYAVPGWLETAHWLDELRRAGKINLIGGTNFDTA
                     HMREIVESGVPLASMQVQYSLLDRRPAKTMAAAAAQHGVFLLCYGAAAGGFLSDKWLG
                     AAEPRGDLENRSLVKYRLIIEEFGGWVLFQALLAALRRIADRHGADIATVASAAALQR
                     ERVAAVIVGARDRSHLAANLAISDLRLTAEDRREIDAVLARAKDLPGDVYALERDRSG
                     RHGAIMKYNLNRDVM"
     gene            2817..>3178
                     /locus_tag="VKU03_12885"
     CDS             2817..>3178
                     /locus_tag="VKU03_12885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172300.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ72209.1"
                     /translation="MRNMLLAAAFLASAAFALPARAADCTITIGMVMELTGPAGAYGQ
                     AGAKAVDMALRDFNEAGGVNGCKIVADTRDSQSQGAVAVDQATQLVNIKKVPVIIGGI
                     ISSVSIPILTSVTAQAGVV"
BASE COUNT          487 a         1073 c         1095 g          523 t
ORIGIN      
        1 tagccggcga ccagcgcgcg cgtttcgagc gccggcggcg caaggaccgg cgcgttcatg
       61 cggcctcgcc gagataggcg gcgatcactt tgcggtcggc cgcgacttcg gccggcgcgc
      121 cttcggcgag ccgtcggccg gaggccatgg cgacgacgcg gccgcagagc cggctgacca
      181 tcgccatatt gtgctcgatc agcatgatcg tcttgccctc ggcgttcaaa gcgcggatgc
      241 gatcgatgat caattcgagc aaagccggat tgacgcccgc cgccggctcg tcgagcagga
      301 tcgcctgcgg gtcggccatc aggatgcggg cgagttcgag cagcttgcgc tggccgccgg
      361 acagcacgcg cgccggttcg ttctccagcc gcgacaggga cacaaagtcg atcaacgccc
      421 gcgctttctc gacggcgcgc ctttcctccg cggcgacgcg gcccggccgc aggaagttca
      481 tcgcgatgcg ctcgccggcc tgccgctggc cgccggtcag cacgttctcc agcacggtca
      541 tcgcggcgaa ggggcgcggg atttgaaacg tgcggccgac gccgagcttg agccggcttt
      601 ccggcccggc ggcggaaacg tcgcggccgg cgatcagaac cgcgccggcg ctcggccgca
      661 agcttcccgc cagcaggttg aacagggtgg tcttgccggc gccgttcggg ccgatcaggc
      721 cgaggatttc gccgcggcga agcgcgatcg acatgccgtc gacggcgctg aggccgccga
      781 attcgcggac cagatcgatc ccctcgatca gcggcggctg ggcgggcttc ccctccgccg
      841 gcttcgactc catcgctcca cccgatattg ctcaaaccgc cgtcccacct atagtggccg
      901 gatgacgacg cgagaagcgg ataaaggcgc tgcggccgac gacgcgttcg acctttacga
      961 tctgcgcgtc gaagtcgtcg cgcccgaagg cggcgcgatt ttttgcggcg ccaggcccgg
     1021 cgaccatttc gaattgcgcg gcgagatgct gtttttgccg cccggtcagg gcttctcgat
     1081 ctattcgctg gcggcgctgc tgccgctgct gccggccaag caacgcccga cccatccaaa
     1141 cgactggatg acgagcgacg ccgaaatcgc ctgtccggac ccgaattgcg cgacgcggtt
     1201 ccgcatccgg cgcttgggcc tgcgtcgctt ccgccatagc gagacgacgg cgacgccgct
     1261 cggcgggaca taaagccgca tggagcgctt tcgcctcgcc cccgattacg agatttcgcg
     1321 ggttatccgc ggcggctggc agatggcggc cggccatggc gcggtgcgcg ccagcgatcc
     1381 gatcgccgat ctcatcgcct tcgccgatgc cggcgtgacg acgttcgatt gcgccgacat
     1441 ctataccggc gtcgaggagc tgatcgggaa tttccggctt cgctaccgcg ccttgcgcgg
     1501 cgaggaggcg gcggggcgca ttcgcgtcca cacgaaattc gtacccgatc tcgatttcct
     1561 gccgcgcatt cagaagagcg acgtcgagcg cgtcatcgac cggtcgctgc gccggctgaa
     1621 catggagcgg ctcgatctcg tccagttcca ttggtgggat tacgccgttc cgggctggct
     1681 ggagacggcg cattggctcg acgagctgcg ccgcgccggc aagatcaacc tgatcggcgg
     1741 caccaatttc gacaccgcgc atatgcggga gattgtcgaa agcggcgtcc cgctcgcctc
     1801 gatgcaggtg caatattcgc tgctcgaccg ccggccggcg aagacgatgg ccgcggccgc
     1861 ggcgcagcat ggcgtctttc tgctctgcta cggcgccgcg gcaggcggtt tcttgagcga
     1921 caaatggctc ggcgccgccg agccgcgcgg ggatctggag aaccgctcgc tggtgaaata
     1981 ccggctgatc atcgaggagt tcggcggctg ggttctgttc caggcgttgc tcgccgcgct
     2041 gcgccgcatc gccgatcgtc acggcgccga tatcgccacg gtcgcgagcg cggcggcgct
     2101 gcagcgcgag cgcgtcgctg cggtgatcgt cggcgcgcgc gaccgctcgc atctcgccgc
     2161 caatctggcg atttcggact tgcggctgac ggcggaggat cggcgcgaga ttgacgccgt
     2221 cctggcgcgg gcgaaggact tgccgggcga tgtctatgcg ctggagcggg accgcagcgg
     2281 gcggcatggg gcgattatga agtacaattt gaatcgggac gtcatgtgag tagcggggcg
     2341 gtaccggtct aactacgcgt gcgcagagcg agcttcgttc tgaaaggctg aggcgccaca
     2401 tcgggccggc gccgtcaagc ggccataacc cgtcccaagc gctggagacg gcgtcgtacc
     2461 tcggcgcggc gggctatggc cgctcctcca cttcgtttcg gcccttcggg tgacggcgcc
     2521 ggcccgatgc ggcatttttg caaccaagaa ctttggtgat gaaaccggcc gcaaagcgcc
     2581 ggtttcatca ccaaagctct tggtcgccaa cggcctcagc cgtgggcggg ggtcaaggct
     2641 cgcggagcgc agcggagcga cgcgcgaagc gcgcttggcc ttgaggcccg cccgcggctc
     2701 aggcagcgcg gcgccctgtg cggcgcgcca tccttgagtt cccgacgccc tggcgcataa
     2761 tcggtttcaa cgatcgcagg ccgagcgcgg ccgccggaca aagggaagga acccccatgc
     2821 gcaacatgct cctcgccgcc gcttttttgg cttcggccgc cttcgccctc ccggcccggg
     2881 ccgccgattg cacgatcacg atcggcatgg tgatggagct gaccgggccg gctggcgcct
     2941 atggccaggc cggcgccaag gccgtcgaca tggcgttgcg cgacttcaac gaggcgggcg
     3001 gcgtcaacgg ctgcaagatc gtcgccgaca cccgcgacag ccagagccag ggcgccgtcg
     3061 cggtcgacca ggcgacgcag ctcgtcaaca tcaagaaggt cccggtgatc atcggcggga
     3121 tcatctcctc ggtgtcgatc ccgatcctga cctcggtcac cgcgcaggcc ggcgtggt
//
LOCUS       DATGJW010000430         3225 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_493853, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000430 DATGJW010000000
VERSION     DATGJW010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3225)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3225)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3225
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_493853"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1985
                     /locus_tag="VKU03_12890"
     CDS             <1..1985
                     /locus_tag="VKU03_12890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72210.1"
                     /translation="ALLRGLAFAALFLAIADPSLTREDRKPLKDVVAVVVDRSDSQSI
                     GDRMVQTDKALVEVRSRLAALGNVETRVIEAGKDDSENQGTRLFSALQSGLSDVPPDR
                     IGGAILITDGVVHDIPASAAALGFKAPVHALITGHEGERDRRIELVEAPRFGIVGKNQ
                     TIVARVLDTADRGEPVSLEVRRDGQKVATLKAEVGETIRVPVAIEHAGPNVVELEVAP
                     IANELTTLNNKAVITIDGVRDKLRVLLVSGEPHQGERMWRNLLKSDANVDLVHFTILR
                     PPEKSVDGTPINELSLIAFPVADLFGRKIKEFDLIILDRYSSQSILPKIYFDNIVSYV
                     RQGGAVLMAEGPDYATPEGLYFSPLGEIAPAKPDGDVIEREFRATISPQGERHPVTRG
                     LAGGASSPPSWGPWFRQIGAEPTAGVNVLNGVDNRPLLVLSRVDKGRIALLLSDQLWL
                     WARGFEGGGPHLDLLRRLAHWLMKEPELEEEAIRASARGKALTIERQSLKDGLPEATV
                     TGPTGETFKAQLKAAEPGLSRATIEVDKFGLYRVSDGERQALVNVGPANPVEFQEVVS
                     TPDHLRALAEATGGTVRRLASGSGDNVAVPSIIAMHAGLNYGGSGYIGVKRTGSSVII
                     GVASMPLAIGFLGLAALLGALVLAWWREGSTSRGKA"
     gene            complement(2089..>3225)
                     /locus_tag="VKU03_12895"
     CDS             complement(2089..>3225)
                     /locus_tag="VKU03_12895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933405.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase"
                     /protein_id="HLJ72211.1"
                     /translation="GRDYIIPVPFDPRLIHVIPAAVAKAAMESGVAQKPIVDLRAYQE
                     QLSARRDPVAGALQRIIERVRRNPKRVVFAEGEEEQVIRAAYSFVTQGLGSAVLVGRE
                     HRVRAKAEAAGFDLSDRDIEIHNAALSSRNAVYAQFLYERLQRRGFLFRDCQRLINQD
                     RNTFAAAMVAQGDADAMVTGVTRNFSVALEEVRRVIDHKPGHRVIGVSLVLARGRPVV
                     VADTAIAEMPTAEDLAQIAIEAAGVARRLGYEPRVAMLAFSSFGHPPGERSMRVQEAV
                     RILDSQRVDFEYDGEMAADVALNRDAMALYPFCRLTEPANVLIMPAFHSASISTKMLQ
                     ELGGATVIGPLIVGLEKPVQIVPLGAKDSDIINMAALASFNIGG"
BASE COUNT          524 a         1094 c         1096 g          511 t
ORIGIN      
        1 gcgcgctgct gcgcggcctc gccttcgccg cgcttttcct ggcgatcgcc gacccgtcgc
       61 tgaccaggga ggaccgcaag ccgctcaagg acgtcgtcgc cgtcgtcgtc gatcgctcgg
      121 acagccagtc gatcggcgat cgaatggtcc aaaccgacaa ggcgctcgtc gaggtgcgga
      181 gccgcctcgc cgctttgggt aacgtcgaga cgcgcgtgat cgaggcgggc aaggacgatt
      241 ccgagaacca gggcacgcgc ctgttctcgg cgctgcaatc cggcctttcc gatgtgccgc
      301 cggatcgcat cggcggcgcc atcctgatca ccgacggcgt cgtccacgac attccggcca
      361 gcgctgcggc gctcggcttc aaggcgccgg tccatgcgct gatcaccggc catgagggcg
      421 agcgcgaccg ccgcatcgag ctcgtcgagg cgccgcgctt cggcattgtc ggcaagaacc
      481 agaccatcgt cgctcgggtg ctcgacaccg ccgaccgcgg cgagcccgtc tctctggagg
      541 tgcggcgcga cggccagaaa gtggcgacgc tgaaggcgga ggtcggcgag acgatccgcg
      601 tgccggtcgc catcgaacat gccgggccga atgtcgtcga gctcgaagtc gcgccgatcg
      661 ccaacgagct gaccacgctg aacaacaagg cggtgatcac aatcgacggc gtgcgcgaca
      721 aattgcgcgt gctgctcgtc tcaggcgagc cgcatcaggg cgagcgcatg tggcgtaatc
      781 tcttgaagtc cgacgccaat gtcgatctcg tccatttcac gattctgcgg ccgccggaaa
      841 aatccgtcga cggcacgccg atcaacgagc tctctttgat cgccttcccg gtcgccgatc
      901 tgttcggccg caagatcaag gaattcgacc tcatcatcct cgaccgctat tcgagccagt
      961 cgatcctgcc gaaaatctat ttcgacaaca tcgtctccta cgttcgccag ggcggcgcgg
     1021 tgctgatggc ggaagggccg gattatgcga ccccggaagg gctctatttc tcgccgctcg
     1081 gcgagatcgc gccggccaag cccgacggcg acgtgatcga gcgcgaattc cgcgcgacga
     1141 tctcgccgca aggcgagcgc catccggtga cgcgcggcct tgccggcggc gcctcctcgc
     1201 cgccttcttg gggaccgtgg ttccgccaga tcggcgctga gccgacggcg ggcgtcaatg
     1261 tcctcaacgg cgtcgacaat cggccgttgc tcgtcctgtc gcgcgtcgac aagggccgca
     1321 tcgcgctgct cctctccgac cagctttggc tctgggcgcg cggcttcgaa ggcggcgggc
     1381 cgcatctcga cctgctgcgc cgtctcgccc actggctgat gaaggagccg gagctcgaag
     1441 aggaagcgat ccgggcgagc gctcgcggca aggcgctgac catcgaacgt cagagcttga
     1501 aggacggttt gccggaggcg accgtcaccg gcccaaccgg cgagacgttc aaggcgcagc
     1561 tcaaagcggc cgagccgggc ctttcgcggg cgacgatcga ggtcgacaag ttcggccttt
     1621 atcgcgtcag cgacggcgag cgccaggccc tcgtcaatgt cggtccggcc aatccggtcg
     1681 agttccagga agtcgtctcg acgcccgacc atttgcgcgc gctcgccgaa gcgaccggcg
     1741 gcacggtgcg gcgactcgcg agcggctccg gcgacaacgt cgccgtgccg agcatcatcg
     1801 cgatgcatgc cggcctgaac tacggcggct cgggctatat cggcgtcaag cgcactgggt
     1861 cgagcgtcat tatcggcgtc gcctcgatgc cgctggcgat cggcttcctc ggcctcgcgg
     1921 cgctgctcgg cgcgctggtc ctggcgtggt ggcgagaggg gtcgacgagc cggggcaagg
     1981 cgtagggctg cgggcggtgt aggcgcggtc gcgctgaccg cgctgcttcc gccggcgcct
     2041 cgcgcgcgcc gcggtcgccg accgcggcta caaggctcgc gccgcgcgct acccgccgat
     2101 attgaagctc gccaacgccg ccatattgat gatgtccgaa tctttcgcgc cgagcggcac
     2161 gatctggacc ggcttctcca ggccgacgat cagcggcccg atcaccgtcg cgccgcccag
     2221 ctcctgcagc atcttggtcg agatcgacgc cgaatggaaa gccggcatga tcaggacatt
     2281 ggccggctcg gtcaggcggc agaacggata gagcgccatc gcgtcgcggt tcagcgcgac
     2341 atcagcggcc atctcgccgt cgtactcgaa atcgacgcgc tgcgaatcaa gaatccgcac
     2401 cgcctcctgc acccgcatcg agcgctcgcc cggcggatgg ccgaacgagg agaaggcgag
     2461 catggcgacg cgaggctcgt agccaagccg gcgggcgacg ccggccgctt cgattgcgat
     2521 ctgcgcgagg tcctccgccg tcggcatttc ggcgattgcc gtgtcggcga cgacgaccgg
     2581 gcggccgcgc gccagaacca gcgacacgcc gatcacccga tggccgggtt tgtgatcgat
     2641 gacgcggcgc acctcttcga gcgccaccga gaaattgcgg gtgacgccgg tgaccatcgc
     2701 atcggcgtcg ccctgcgcca ccatggcggc ggcgaacgtg ttgcggtcct ggttgatcag
     2761 acgctggcag tcgcggaaaa gaaagccgcg ccgctgcagg cgttcataga ggaattgcgc
     2821 atagacggcg ttgcgcgacg acagggccgc attgtgaatc tcgatgtcgc ggtcgctcaa
     2881 gtcgaagccg gccgcctcgg ctttggcgcg cacgcggtgc tcgcggccga ccagaaccgc
     2941 cgagccgagg ccctgcgtga cgaaggaata ggcggcgcgg atcacctgct cttcctcgcc
     3001 ctcggcgaag acgacgcgct tgggattgcg cctgacccgc tcgatgatcc gctgcagggc
     3061 gccggcgacc gggtcgcgcc gcgccgagag ctgctcctgg taggcgcgca gatcgacgat
     3121 cggcttttgc gcgacgccgg attccatcgc cgccttggcg accgcggccg ggatgacatg
     3181 gatcaggcgc ggatcgaacg gaacgggaat gatgtagtcg cggcc
//
LOCUS       DATGJW010000431         4868 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_84499, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000431 DATGJW010000000
VERSION     DATGJW010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4868)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4868)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4868
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84499"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1056)
                     /locus_tag="VKU03_12900"
     CDS             complement(<1..1056)
                     /locus_tag="VKU03_12900"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ72212.1"
                     /translation="MKLVVFGLTVTSSWGNGHATLWRGLQRALARRGWTVVFFEHDTP
                     YYAQNRDLHELPNGELILYREWAEAQALARRHVAEADVAMVTSYCPDGVAAGDLVLAA
                     PRALRAFYDLDTPVTLARLRQGEPLAYIGPRGLGDYDLVLSYTGGAALDDLGLLAGAR
                     RAVPLYGHVDPEKHYPTRPESRYRAHLSYVGTYAADRQPALQRLFIDPARRRPDLRFV
                     LAGAQYPEGFPWTKNIFFVRHLPPSEHSAFYASSRLTLNVTRRDMAAMGWCPSGRLFE
                     AAACGAAIVSDSWEGLETFFRPEREIIVAGGCTDVIAALDLPDHELRRVGAAARERVL
                     AEHTSAHRAAELEKFFTE"
     gene            complement(1053..1676)
                     /locus_tag="VKU03_12905"
     CDS             complement(1053..1676)
                     /locus_tag="VKU03_12905"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLJ72213.1"
                     /translation="MTIFYLIRHAAQDDLAVRLAGRAPGAALGEAGKAQARLLGERMR
                     VERLDHIHASPRQRATETARAIEAARGGELRVEIAEALDEVDFGAWSGCDFADLARDM
                     AWRCWNERRSLARAPGGESMLDVQTRIMGHIERVSAAVPEGAVAVVSHAEVIRAALLY
                     CLGLSLDAWPLLEISPASISRLSLTALGAKILGVNETVAIKGAEGRA"
     gene            complement(1673..2767)
                     /locus_tag="VKU03_12910"
                     /pseudo
     CDS             complement(1673..2767)
                     /locus_tag="VKU03_12910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453183.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="inositol-3-phosphate synthase"
     gene            3127..>4868
                     /locus_tag="VKU03_12915"
     CDS             3127..>4868
                     /locus_tag="VKU03_12915"
                     /inference="COORDINATES: protein motif:HMM:NF012204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="leukotoxin LktA family filamentous adhesin"
                     /protein_id="HLJ72214.1"
                     /translation="MRSSALLPVAFVLFGASSAFGQAVNLGRAAGNAIIPDGRTATTL
                     SGNGRVTNITTTTLRGGNAYNSFSQFREARGNVVNLVVPNAARNLVNVVTDGAVDIQG
                     ALNSYKNGVIGGNVVFADSYGFIVGKTGVVNVGALTVVTPAKSALDRLISANGAIDSK
                     VANQIIHGDVPLSPDGSVSISGQIVAQHGVRINAHDVAVAGSIPEAERVARQKALFES
                     TVNAVGLREGGAIVARHGGIEIVATNNIGVDGSLAAGGSRRHAASISLSAGHSVSIGA
                     SADIRATGRNSHVAIASGDDIAIAGAIAAKAAPGENAGRIDVSAVGDVSIAESAAFDA
                     AGAGLKANGGAISIKPSGNLDVADGATFDASAGAFGNGGLVELSAGKVATIGDIRLNL
                     GAPGGEAGTLLLDPWDLQIGGAAAGGSSTASDVTVDSSFSPSLITHGASVVLEADHSI
                     TIAAGGVIDTTLSGGQSGAIALTAPSISVLGGAQLLADAVGGGTAGAVTLKAIANAAS
                     AGVAQIEIGDSSGLAAVVKGGDILLQATATLAQATDHAIIAINSADVTAAGTLQAQAT
                     ASGGTAVNAVAAVAS"
BASE COUNT          838 a         1582 c         1639 g          809 t
ORIGIN      
        1 ctcggtgaag aatttttcga gttcggcggc gcggtgagcg gacgtgtgct ccgccaggac
       61 gcgctcgcgc gccgccgcgc cgacgcgccg caattcgtgg tcgggaaggt cgagcgctgc
      121 gatcacgtcg gtgcagccgc cggcaacgat gatctctcgt tccggccgaa aaaaggtttc
      181 gagtccttcc caggaatcgc tgacgatcgc cgcgccgcag gcggccgctt cgaagagccg
      241 gcccgacggg caccagccca tcgccgccat gtcgcgccgg gtcacgttga gggtgaggcg
      301 cgaggacgcg tagaaggccg aatgctcgct cggcggcaga tgacggacga agaagatgtt
      361 cttcgtccat ggaaagcctt cgggatattg cgcgcccgca agaacgaagc gcagatcggg
      421 gcggcgccgg gccgggtcga tgaacaggcg ctgcaaggcc ggctggcggt cggcggcata
      481 ggtgccaaca taggaaagat gggcgcgata gcgcgactcc ggacgggtcg gatagtgctt
      541 ttccggatcg acatgaccgt aaagagggac ggcgcgccgc gcgccggcca gaaggccgag
      601 atcgtcgagc gcggcgccgc cggtatagct gagaacgagg tcgtaatcgc cgaggccgcg
      661 cggcccgata taggcgagcg gctcgccttg gcgcagccgg gcgagcgtga ccggcgtatc
      721 gagatcgtaa aaggcgcgca aggcgcgcgg cgccgcgagc accagatcgc ccgccgcgac
      781 gccatctggg caataggagg tgaccatggc cacgtcggcc tcggcgacat ggcgccgcgc
      841 cagggcctga gcctcggccc attcccgata gaggatcagt tcgccattcg gcagttcgtg
      901 caggtcgcga ttttgagcgt agtagggcgt atcgtgctcg aagaagacca ccgtccaacc
      961 gcgccgcgcc aaggcgcgct gcaatccgcg ccacaaggtc gcgtgcccgt tgccccagga
     1021 cgatgtgacg gtcagaccga aaacgacgag cttcacgcgc ggccctccgc gcccttgatc
     1081 gcgacggtct cgttgacgcc gagaatcttt gcgccgagcg ccgtcaagga gaggcgcgaa
     1141 atcgaagccg gcgaaatctc cagcaacggc cacgcgtcga gagagaggcc gaggcaatag
     1201 agcagcgcgg cgcggatgac ctcggcgtgg ctgaccaccg cgaccgcgcc ttcaggaacg
     1261 gcggcgctga cgcgctcgat atgacccata attcgcgttt ggacgtccag catgctctcg
     1321 ccgccaggcg cgcgggcgag gctgcggcgc tcgttccagc agcgccatgc catgtccctg
     1381 gcgaggtcgg cgaaatcgca gcccgaccag gcgccgaaat cgacctcgtc caaagcctcg
     1441 gcgatctcga cccgcaattc gccgccgcgc gccgcctcga tcgcgcgcgc ggtttccgtt
     1501 gcccgctggc gcgggctcgc atggatatga tcgagccgtt cgacgcgcat tcgctctccc
     1561 aaaagacgcg cctgagcttt cccggcttct ccgagagccg cgccaggcgc tctgcccgcc
     1621 aatcgaacgg cgaggtcgtc ctgagccgcg tgccgaatca gatagaaaat cgtcacgaca
     1681 agcggcgagg tctcgcgcca tccccgtcgg cgatgaatcc cagcgtccgt tcgcgcgctt
     1741 cggcgtcggt gaattgctcc ggcggagact tcatgaagta gctggacgga ccgatcagcg
     1801 cgccgccctg gccgcggtcg agcgccagct tggcgcagcg caccgcgtcg atgacgacgc
     1861 cggccgagtt gggagaatcc cagacttcca gcttgacctc cgcattgagc ggcaggccgc
     1921 cgaatgtcgt accctccata cggatatggc agaacttccg gtcggtcagc catggaacga
     1981 agtcgcttgg gccgacatgg acgttttccg cgtcgagcgg cgcgtcgagc tggctcatca
     2041 ccgactgggt cttcgaaagt tttttcgatt cgagccgctc ccgctccagc atgttgcgga
     2101 aatcggtatt gccgccgaaa ttgagctggt aggtcctgtc aagcctgacg ccgcgctcgc
     2161 ggaaaagatt agtcaagacg cgatggacga tggtcgcgcc gacttggctc ttgatgtcgt
     2221 cgccgaggag cggcagcttt tgccgctcga aacgctggcg ccattgcggc ttggaggcga
     2281 tgaagacggg aatgcaattg acgaaggcgc agcccgcctt cagcgtctgc tcggcataaa
     2341 attccgtcgc tttttgcgat ccgaccggca gatagcagac gacgacgtcg gtctgcgtcg
     2401 ctttcagcgt ctcggcgacg tcgcagtcgg gcgcgtcgga ctcttcgatt tcgtcgcgca
     2461 aatatttgcc gatcccgtcg aatgtcgggc cgcgccgcac cgtcacgccg gtcttggcga
     2521 cgtcagcgaa gcgataggtg ttgttcggcc cggcgtagat cgcctcggcg acgtccttgc
     2581 cgaccttcgt cgtcgaaggc ggaggcgatc tcgacatcgt caatgcgata gccgccgatc
     2641 gaaatgctca tcaggccggg cggcgctcgt ttgaggcggc gccgcgataa taggtgagcc
     2701 cctgcaccag cgaggacgcg caattgccca cgccgactat gccgacgcgc accttcttgg
     2761 agctcaacgg cccctcctta agcgcaaatc aaattaccgg gccaacgccc ggcgttgggg
     2821 gaagttccgc cgcgcggtcg cttggagccc gcgacgcctt ctgtgcgtcg cactgtcgcc
     2881 gcgaaatgtc cttgtgagcg ctgctgaaat ctgctttcac tagcaaactg gagggttgcg
     2941 ccgcggactg gcggcgcatg ggagggccct ttagcgaaaa tttgacgctt tacgttgccg
     3001 ctcggatggg tgcggcgtcg gccgaatcgc tggcgctgaa tcgcagaatc cccgcgggcg
     3061 gaggggaaag gaatgtctcc aggggctaag cgcgtcgcag cggggggacg cgcgaaacgg
     3121 gctctgatgc gaagctcggc gctgcttcct gtcgccttcg tattattcgg cgcgtcttcg
     3181 gctttcggcc aggccgttaa ccttgggcgc gcggccggaa acgccatcat tccggacggg
     3241 cgaaccgcga ccacgctcag cggcaatggc cgcgtcacca acatcaccac gacgactctg
     3301 cgcggcggca acgcctacaa ctcgttctcg cagttccgcg aggcgcgcgg caacgtcgtt
     3361 aaccttgtgg tgccgaacgc agcgcgaaat ctcgtcaatg tggtcaccga cggcgcggtc
     3421 gatattcagg gcgctctcaa ttcgtataaa aacggcgtga tcggcggcaa tgtcgtcttc
     3481 gccgattcct atggtttcat cgtcggcaag acaggcgtcg tcaatgtcgg cgcgctgacg
     3541 gtcgttacgc ccgcgaagag cgcgctcgac aggctgatca gcgccaatgg cgcgatcgac
     3601 tcgaaggtcg ccaatcaaat catccatggc gatgtgccgc tttcgccgga cggctcagtt
     3661 tcgatcagcg gccagatcgt cgcgcagcac ggggtcagga ttaacgccca cgacgtcgcc
     3721 gtggccggat cgattcccga ggcggagcgc gtcgcgcgtc agaaggcgct gttcgaatcg
     3781 acggtcaacg cggtcggctt gcgcgagggc ggcgccatcg tcgcccgcca tggcggtatc
     3841 gagatcgtcg ccacaaacaa tatcggggtt gacggctcgc tcgccgccgg cggctcgcgg
     3901 cgccacgccg cgtcgatctc gctgtccgcc ggccattcgg tctcgatcgg cgcgtcggcg
     3961 gatatccgcg ccacgggccg caattcccat gtcgcgatcg cctcaggcga cgatatcgcg
     4021 atcgccggcg ccatcgccgc aaaggcggcg cctggagaaa acgcggggcg catcgatgtt
     4081 tccgccgtag gcgatgtcag catcgccgag tccgcagcgt tcgatgcggc tggcgccggg
     4141 ctcaaggcga atggcggggc gatttcgatc aagccgagcg gcaatcttga tgtcgccgac
     4201 ggcgccacgt tcgacgccag cgccggcgcc ttcggcaatg gcggcctggt cgagctcagc
     4261 gccgggaaag tcgcgacgat cggcgacatt cggctgaatc ttggcgcgcc gggcggcgaa
     4321 gccggcacgc tgctgctcga tccctgggac ttgcagatcg gcggcgccgc cgccggcggc
     4381 tcgtcgacgg cttccgacgt taccgtcgat tcgagcttct cgcccagcct gatcacgcat
     4441 ggcgcgagcg tcgttctgga agccgatcat tcgatcacca tcgccgccgg cggggtcatc
     4501 gacacgaccc tttcgggcgg ccaatccggc gccatcgcgc tgaccgcgcc atcgatcagc
     4561 gtgctgggcg gcgcgcaact gctcgccgat gcggtcggcg gcgggacggc gggcgccgtt
     4621 acgctgaagg cgattgcgaa tgcggcgagc gccggcgtcg cgcaaatcga gatcggcgat
     4681 tcctcaggct tggccgccgt cgtcaagggc ggcgacatcc tgctgcaggc gaccgcgacg
     4741 ctcgcccagg cgaccgatca cgcgatcatc gcgattaact cggccgacgt gacggcggcc
     4801 ggaactttgc aggcccaggc gacggccagc gggggaaccg ccgtcaatgc ggtcgcagcc
     4861 gtcgcctc
//
LOCUS       DATGJW010000432         4510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1433977, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000432 DATGJW010000000
VERSION     DATGJW010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4510
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1433977"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1074)
                     /locus_tag="VKU03_12920"
     CDS             complement(<1..1074)
                     /locus_tag="VKU03_12920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003548944.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72215.1"
                     /translation="MKLHYGWIVVAVGALMTCVGMGAVFSLPIFLQPITEATGWSRAG
                     VSGAMTIDFVTMGVAGFGWGALSDRFGARPIVFAGACLLGLGLVLASRATSLLEFQLV
                     YGVLLGLAGGSFFTPMIATVTGWFDQHRSLAVSLVSAGMGVAPMTVSPFARWLISATD
                     WRTAQFIIGVAAFLLLAPAALLVRRPPVAATTPDAAPADNSGMSVAQAFRSPQFIVLA
                     LTFFACCAAHSGPIFHTVTYAMGCGIPAMTAVTIYSVEGLAGLGGRVLLGYLGDRLGA
                     KPVLVAGLIVQAVAAGSYLLAGNIVQFYGVAFVFGLAYGGVMPLYAVLARDYFGPKIM
                     GTIFGAAAMVSSLGMALGPSVGGW"
     gene            complement(1192..1803)
                     /locus_tag="VKU03_12925"
     CDS             complement(1192..1803)
                     /locus_tag="VKU03_12925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011173.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ72216.1"
                     /translation="MTAKLTARDKLLDAAIATVRAKGYSATSVDDLCAGAGVTKGAFF
                     HHFKSKEALGVSAAARWSENADALFAAAPYHAHADPLDRLLGYIDYRKAILQGAIAEF
                     TCLIGTMVQEAYETHPAIREACERAIVGHAAKLAPDIAEAMRAHGIKGDWTAESLALH
                     TQAVLQGAFILAKATGGAKAAADTVDHLRRYVELLFDRRPGRE"
     gene            complement(1871..2800)
                     /locus_tag="VKU03_12930"
     CDS             complement(1871..2800)
                     /locus_tag="VKU03_12930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017994112.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ72217.1"
                     /translation="MSQPDLNLLIALDALLAESSVSGAARRLGLSASAMSRTLARLRA
                     ATGDPLLTPAGRAMVPTPHAEALRERVSELSQAVQAVLRPAPNIDIQRLERTFVIRAN
                     EGFVEVFAARLVAAANAQAPGVRLRFAPKPNKDPRPLREGQIDLDIGVLEDTAPELRV
                     QAMFRDVFVGVARAEHPLLKGAITPERFAAFDHVVASRSGRAQGPVDEALAALGLVRN
                     IAAVVPSFPAVFAVVSTSDMIGLAPRSYFSAGPTSEPSRRPAVSSFPLPVRTQGITVS
                     QMWHPRLDADAAHRWLRGLILDLCRKPDSATAG"
     gene            2919..4349
                     /locus_tag="VKU03_12935"
     CDS             2919..4349
                     /locus_tag="VKU03_12935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011424334.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72218.1"
                     /translation="MSSVLSAEIAARGKRDPQTPALGAAPRAVEDGLPLPRRAVALGA
                     LLAATVLVVLDGAIANVALPSIAHALDVSSADCVWVVAAYQLGVVVALLPCAALGESY
                     GGRKVFVAGISVFAAASALCALAPNLPLLAAARFIQGIGGGAIMALGPMMLRFASPQR
                     LIGRVIAWNAMVIALSSAAGPGVGAAILAIADWPWLFAVNLPIAALVLVAGRALPGPR
                     GTGCSLDLVSAAINTCMFALFVFGGDRIAAQPGRGAAMILGAVLCLAALVRRERAREA
                     PLVPIDLLRSGAFRLAAIASVCCFAAQMMGIVALPFYFQRELGESVVATGLLMTPWPI
                     AVALAAPISARLSDRVSTALICAAGGACLALGLALAGAWPLRHDLAPIIVGTILAGIG
                     FGLFQTANNRILLLSAPKARSGAAGGAQGTARLLGQTIGAVGMGLLFELASAGAAPRI
                     GLVVAALCALSASGVSALRLRREGRG"
BASE COUNT          756 a         1486 c         1518 g          750 t
ORIGIN      
        1 ccacccgcca accgacggac cgagcgccat gccgaggctg gagaccatgg cggcggcgcc
       61 gaagatcgtg cccatgatct tcgggccgaa atagtcgcgc gccagcacgg cgtagagcgg
      121 catcacgccg ccataggcaa ggccgaagac gaaggcgaca ccgtagaatt gtacgatatt
      181 gccggcgagg aggtacgatc ccgccgccac ggcctgcaca ataaggcctg cgacgagaac
      241 tggcttggcg cccaagcggt cgccgagata accgagcagc acgcgcccgc ccaatccggc
      301 gagcccctcg acgctataga ttgtcaccgc ggtcatggcc ggtatgccgc agcccatggc
      361 gtaggtaacc gtgtggaaga tcgggccgga atgggccgcg caacaggcga agaatgtcaa
      421 agccaggacg atgaattgcg gcgagcggaa agcttgcgca acgctcatgc cggaattgtc
      481 ggccggcgcg gcgtcgggcg tcgttgccgc gacgggcggc cggcgcacca gcaaagcggc
      541 gggcgcgagc agcaggaagg cggcgacgcc gatgatgaat tgcgccgtac gccaatccgt
      601 ggcgctgatc agccagcgcg cgaaaggcga gaccgtcatc ggcgcgacgc ccatgccggc
      661 cgagaccagg gagacggcaa gactgcggtg ttggtcgaac cagccggtca ccgtcgcgat
      721 catcggcgtg aagaaactgc caccggcaag gccgagcagc acgccataga cgagttggaa
      781 ttcgagaagg ctcgtcgcgc ggctggcgag tacgaggcct aaccctaaga ggcaggcgcc
      841 ggcgaagacg atgggccgcg cgccgaaacg atcgctcaaa gcgccccagc cgaaaccggc
      901 gacgcccata gttacaaaat cgatcgtcat cgcgccggag acgccagccc gagaccagcc
      961 tgtcgcctcc gtaatcggct gcaggaagat cggcagcgag aaaaccgcgc ccatgccgac
     1021 acaagtcatc aaagcgccga cggcgacgac gatccaaccg taatgcagct tcatgtcggg
     1081 cctcttcaat ggaactggct catctgcctc tggcaagtac aaccgcgcga accacccctt
     1141 tcccgcttgc gggagaagat ggcgcgaagc gccggatgag ggtaagccgc atcattcgcg
     1201 accgggtcga cgatcgaaca acagttcgac gtaccgtctc agatgatcga ccgtatcagc
     1261 agccgccttg gccccgccgg tcgccttggc gaggatgaag gcgccttgca ggaccgcttg
     1321 cgtgtgaagc gcgaggctct cagccgtcca atcgcctttg ataccatgcg cgcgcatggc
     1381 ttcggcgatg tcgggggcga gcttggcggc gtgaccaacg atggcgcgct cgcaggcttc
     1441 gcggatcgct ggatgggtct cgtaagcctc ctgcaccatc gtgccgatga ggcaggtgaa
     1501 ctcggctatc gcgccctgca ggatcgcttt gcgatagtcg atatagccga gcaggcgatc
     1561 gagcggatcg gcatgggcgt ggtagggcgc tgcggcaaaa agcgcatccg cattctccga
     1621 ccaacgcgcc gccgctgaga cgccgagcgc ctctttgctt ttgaaatgat ggaagaaagc
     1681 gcccttggtg acgcccgccc cggcgcagag gtcgtctacg gatgtcgcgg aatagccctt
     1741 ggcgcggacg gtcgcgatgg cggcgtcgag gagcttgtcg cgggcggtga gtttggccgt
     1801 catcacctgt cgataccaac cggttggtat ctcgtcaaga tagcgaggaa atcgccttct
     1861 ggctcccata ttatccggcg gtcgcgctgt ccggctttcg gcacaagtcg aggatcagcc
     1921 cacgcagcca gcgatgcgcg gcgtcggcgt cgagacgcgg atgccacatt tgcgaaaccg
     1981 taatcccttg cgtgcgcacc ggcagcggaa agcttgagac cgccggtcgg cgcgaaggct
     2041 cgctcgtcgg gccggcgctg aaataggatc gcggcgcaag tccgatcatg tcggatgtcg
     2101 agacgacggc gaacacggcg ggaaagctcg gtacgacggc ggcgatgttc cgtaccaaac
     2161 cgagcgcagc gagcgcctcg tctacagggc cttgcgcgcg cccgcttcgc gacgccacga
     2221 catggtcgaa ggcggcgaac cgctcgggcg tgatcgcgcc cttcagcaaa ggatgctccg
     2281 ctctcgcgac cccgacaaag acatcgcgaa acatggcctg tacacgcaat tcgggcgcgg
     2341 tgtcctcgag cacgccgatg tcgagatcga tctgtccttc gcgcaacggc cgcgggtcct
     2401 tgttgggctt gggcgcgaag cgcagcctta cgcccggcgc ctgcgcattt gccgccgcga
     2461 cgaggcgcgc ggcgaagact tcgacgaatc cctcattggc gcgaatgacg aaggtacgct
     2521 ccaggcgctg aatgtcgatg ttcggcgccg gccgcaaaac ggcctgaacg gcttggctca
     2581 attcgctcac ccgctcgcgc agcgcctcgg catgcggggt cgggaccatg gcgcggcccg
     2641 ccggcgtcaa cagcgggtcg ccggtggcgg cccgtagccg cgcaagcgtg cggctcatcg
     2701 ccgaagcgct caagcccagc cgccgcgcgg cgcccgaaac gctgctctcg gccagaagcg
     2761 cgtcgagcgc gattagcagg ttcaggtccg gttgcgacat agcgggatct taacacgacg
     2821 tatggcgtct gatgcaatgg ttgattgtat ccgatgcgcc ttccgccata gctgctgatg
     2881 cctatccttc agcggcaact ggtccggagg cttcccaaat gtcgtcagtc ctatcagcag
     2941 aaatcgccgc tcgaggaaaa cgcgatcctc aaacgccggc gctcggcgcc gcgccgcggg
     3001 cggtcgagga cggactgcct ctgccgcgcc gcgcggtggc gctcggcgcg cttctcgcgg
     3061 cgaccgtgct ggtcgtgctc gacggcgcga tcgccaatgt cgcccttccg tcgatcgccc
     3121 atgcgcttga cgtgtcctcg gccgattgcg tctgggtggt cgccgcctac cagctcggtg
     3181 tggttgtcgc cctgctgccc tgcgccgccc tgggcgagag ttacggcggc cgcaaggtct
     3241 tcgttgcggg catatctgtg ttcgcggcgg cctccgccct ctgcgcgctt gcgcccaatc
     3301 ttcccctcct ggccgcggcg cgcttcattc aggggattgg cggcggcgcg atcatggcgc
     3361 ttggcccgat gatgctgcgc tttgcctctc cgcaacgcct gatcggccgc gtcatcgcct
     3421 ggaacgcgat ggtcatcgcg ctttcgtccg ccgccgggcc gggggtcggt gctgcgatcc
     3481 tcgccatcgc cgattggccc tggctctttg ccgtcaatct gccgatcgcc gcgcttgtgc
     3541 tcgtcgccgg acgcgctttg ccgggccctc gtggaaccgg ctgctcgctg gatctcgtca
     3601 gcgcggcgat caacacttgc atgttcgcgc tcttcgtttt cggcggcgac agaatcgcgg
     3661 cgcagccggg acgaggcgcg gcgatgattc ttggcgcggt cttatgcctt gcggcgcttg
     3721 tccgccgcga gcgagcgcgc gaagcgccgc tcgtacccat cgaccttttg cgcagcggcg
     3781 cattccggct cgccgcgatc gcctcggtct gctgcttcgc cgcgcagatg atgggtatcg
     3841 tggcgctgcc cttctacttc cagcgcgaac tcggcgagag cgtcgtcgcg acaggactct
     3901 tgatgacgcc ctggccaatc gcggtggcgc tcgccgcgcc gatttccgcc cgcctgtccg
     3961 atcgcgtctc gactgcgctc atatgcgccg ctggcggcgc ttgcctggcc ctcggcctgg
     4021 cgctcgccgg agcctggccg ctgcgccacg atctggcgcc gatcatcgtc ggcacgatcc
     4081 tggcggggat cggcttcggc ctcttccaga ccgccaacaa tcgcatcctg ctcctctcgg
     4141 cgcccaaggc gcgcagcggc gcggccggcg gcgcgcaagg tacggcgcgg ctgctaggcc
     4201 agacgattgg cgcggttggg atggggttgc ttttcgaact agcgtcggcc ggcgccgcgc
     4261 cgcggatcgg gctggtcgtc gcagctttgt gcgcgctgag cgcgagcggg gtgagcgcgc
     4321 tgcggttgcg gcgggagggg agggggtgat cttcgaacgc tttgcggtga gagggaaacg
     4381 tacaacaatg acggctcgcg gcttaccctc atcccaacct tctcccgcaa gcgggagaag
     4441 gggcgcgtcg cggctgtcct cgcggaggaa gcatttccag ctcaggctcc gcgacatcga
     4501 cggacaagca
//
LOCUS       DATGJW010000433         3585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1123481, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000433 DATGJW010000000
VERSION     DATGJW010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3585
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123481"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..408
                     /locus_tag="VKU03_12940"
     CDS             <1..408
                     /locus_tag="VKU03_12940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016845519.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding protein"
                     /protein_id="HLJ72219.1"
                     /translation="LVAGVWLGNDDGSPTKKTSGGNLPVEIWSRFMKAALQGVPPAGL
                     PSGVWKPATPLDAPLAPPANIASVPPLSDAPRGQPMVINRAAEAAAAPAQPATIPVRS
                     NVLLPPAPIPNAGAPRVVPRKEKSFLDKLFGGA"
     gene            complement(485..1711)
                     /locus_tag="VKU03_12945"
     CDS             complement(485..1711)
                     /locus_tag="VKU03_12945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018425676.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLJ72220.1"
                     /translation="MEHAVIIGAGHGGAQAAASLREEGFQGRITLVSDERVAPYHRPP
                     LSKAFLKDPSAGPQFLRGPKFFAEHGVDLLLQTRATTLDPAASRVMLSNGGALEFSHL
                     VLALGGKPRRLAAPGGDLPGLYSLLSLPDAEALRHALQKGGEAAVIGGGFIGLEVAST
                     LVALGFKTTVIEAMPRVLGRAVAPLISEHMLRRHRGWGAEILLNEGVERIEGDAKGPT
                     AVVTRSGRRIGASLVVVGVGMVPNVALAEAAGLAVDNGVAVDAHLRAADPRIFAIGDC
                     ASYPHWSAARRLRLESVQNATDHGRTVAKAIVGRPEPYRAAPWFWSDQHNIKLQMVGL
                     SFEADDYILSGDPEADRFSAYHFRGPRLVAIDSVNSPAEHMLGRRMLAAGFSPAKEAA
                     KGGAAALKAVFMTKGE"
     gene            1857..2744
                     /locus_tag="VKU03_12950"
     CDS             1857..2744
                     /locus_tag="VKU03_12950"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891665.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HLJ72221.1"
                     /translation="MPLSSMTGFARAAGAAGAYRWAFELKSVNAKGLDLRLRLPPGFD
                     RIEAEARARLGKALARGTCFATLTAQREGGSGEAKINMGTLTALAQAARLAAEQAGLA
                     PPTIDGLLGLRGVVDFVEAAEEEDNLAAACAGALKSLDEAIAALVAMRQNEGAALAAF
                     LDERLTAIAALTQAADENPGRRPEAIRERLAQSVAALLESSRGFDENRLHQEAVLLAA
                     KADIREELDRLKTHVAATRDLIRQGGPVGRRLDFLAQELAREANTLCAKSNDVALTAI
                     GLDLRSQIEQLREQIQNVE"
     gene            2753..3400
                     /gene="gmk"
                     /locus_tag="VKU03_12955"
     CDS             2753..3400
                     /gene="gmk"
                     /locus_tag="VKU03_12955"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008128521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLJ72222.1"
                     /translation="MATKRRGLLLILSSPSGAGKTTLTRMLLQDKALDLTLSVSVTTR
                     ERRSSEADGIHYHFIGARQFEAMRAAGDLLESADVHGHRYGTPRAPVENVLSEGRDML
                     FDIDWQGAQQLRDAMGADVVSIFILPPSMRELKARLDRRAEDAAAQIAARLDNARKEI
                     ARWRQYDYVLINDDLQRAYSAVVSIITAERLRRHRVEKGVETFVKGLLDDSVGGS"
BASE COUNT          593 a         1243 c         1206 g          543 t
ORIGIN      
        1 ctcgtcgccg gcgtctggct cggcaatgac gacggctcgc cgacgaaaaa gacctccggc
       61 ggcaatttgc cggtcgaaat ctggagccgc ttcatgaaag cggcgttgca aggcgtgccg
      121 cccgccggcc tgccctcggg cgtctggaag cccgcgacgc cgctcgacgc gccgcttgcg
      181 ccgcccgcca atattgcgtc tgtcccgccg ctgtccgacg ccccgcgcgg ccagccgatg
      241 gtcatcaacc gcgccgcgga ggccgccgcg gctccggcgc agccggcgac gatcccggtg
      301 cgcagcaacg tgctgctgcc gcccgcgccg atcccgaacg ccggcgcgcc gcgcgttgtc
      361 ccgcgcaagg agaagagctt cctcgacaag ctgttcggcg gcgcgtgaag aggaatggcg
      421 aatagcgagt ggcgaatgag ggtacaaaga ctcgccattc gccattcccc attcgcccca
      481 ttccttattc gcccttcgtc atgaagaccg ccttcagcgc cgccgcgccg cctttcgccg
      541 cctccttcgc cgggctgaag ccagcggcca gcatgcggcg gccgagcatg tgctcggccg
      601 ggctgttgac ggaatcgatc gcgacaaggc gcggcccgcg gaaatggtac gcggagaatc
      661 ggtccgcctc gggatcgccg gagagtatgt agtcgtcagc ctcgaaagaa agcccgacca
      721 tctgcaattt gatgttgtgc tgatcggacc agaaccacgg cgccgcgcgg tacggctcgg
      781 gccggccgac gatcgccttg gcgacggtgc ggccgtgatc ggtggcgttc tgaacggatt
      841 cgaggcgcag ccggcgcgcc gcgctccaat gcggataaga ggcgcagtcg ccgatcgcga
      901 aaatccgcgg atcggcggcg cgcaaatgcg cgtcgacagc aacgccattg tcgaccgcca
      961 gtcccgccgc ctcggcgagc gcgacattgg ggaccatgcc gacgccgaca acgaccagcg
     1021 aagcgccgat ccgccgcccc gatcgtgtga cgacggcagt cggtcctttg gcgtcgcctt
     1081 cgatccgctc gacgccttca ttgagcagga tttcggcgcc ccagccgcgg tggcggcgca
     1141 gcatatgctc ggaaatcagg ggggccacgg cgcggccgag cacgcgcggc atggcctcga
     1201 tcaccgtcgt cttgaagccg agcgcgacca gggtcgaggc gacttccagc ccgatgaagc
     1261 cgccgccgat caccgcggct tcgccgcctt tttgaagagc gtgccgcaaa gcttcggcgt
     1321 cggggagcga gagcagcgaa tagaggcccg gcaagtcgcc gcccggcgcg gcgaggcggc
     1381 gcggcttgcc gccgagcgcc aacacgagat gggagaattc caacgcgccg ccattggaca
     1441 gcatgacccg gcttgcggcg ggatcgagcg tcgtcgcccg cgtctgcagg agcagatcga
     1501 cgccatgctc ggcgaagaat ttcggcccgc gcaggaattg cggaccggcc gaggggtctt
     1561 tcagaaacgc cttggagagc ggcggcctgt ggtagggcgc gacccgctcg tcgctgacga
     1621 gcgtgatgcg gccctggaac ccttcttcgc gcaagctcgc cgccgcctgc gcgccgccat
     1681 ggccggcgcc gataatcacc gcatgctcca tgccgcctct ccgttcggcc aatgtggagg
     1741 ggttctagcc gccgtcgccg accgcggcta cagccgtcgt cagacccgag gttgagcctc
     1801 gttgtcgctc cgcctatggt cggcgcccgt cactcaagcc gagtcgcgaa aaatccatgc
     1861 ctctttcgag tatgaccggc ttcgcccgcg cggccggcgc cgcaggcgcc tatcgctggg
     1921 cgttcgaatt gaagagcgtc aacgccaagg gcctcgatct gcgtttgcgc ctgccgccgg
     1981 gcttcgaccg gatcgaggcc gaggcgcgcg cccggctcgg caaggcgctg gcgcgtggga
     2041 cctgcttcgc cactttgacc gcgcagcgcg aaggcgggag cggcgaagcg aagatcaata
     2101 tggggacctt gacggcgctc gcccaagccg cccggctggc ggccgaacag gccgggctgg
     2161 cgccgccgac gatcgacggg ctcctcggcc tgcgcggcgt cgtggacttc gtcgaagcgg
     2221 ccgaggagga ggataacctc gccgccgcct gcgccggcgc gctgaagagc ctcgatgagg
     2281 cgatcgccgc gctcgtcgcc atgcgccaaa acgagggcgc ggcgctggcg gcgttcctcg
     2341 acgagcggct gacagcgatc gcggcgctca cccaagccgc cgacgagaat cccggccgcc
     2401 ggcccgaggc gatccgcgag cgcctcgccc aatcggtcgc cgctttgctc gaatccagcc
     2461 gcggtttcga cgagaatcga cttcatcagg aggccgtgct gctcgccgcc aaggccgaca
     2521 ttcgcgagga gctcgaccgg ctgaagacgc atgtggcggc gacgcgcgac ctgatcaggc
     2581 agggcggccc ggtcggccgg cggctcgatt tcctcgcgca ggagctggcg cgcgaagcca
     2641 acacgctctg cgccaaatcg aacgacgtcg cgctgaccgc gatcggcctc gacttgcgca
     2701 gccagatcga gcaattgcgc gagcagattc agaacgtcga gtgattccgg ctatggcgac
     2761 caagcgacgc ggcctcctct tgatcctctc ttcgccttcc ggcgccggca agacgacgct
     2821 gacccgcatg ctgctgcagg acaaggcgct cgatctcacc ctttcggttt cagtgacgac
     2881 gcgcgagcgc cgctcgtcgg aagccgacgg catccactat catttcatcg gcgcgcggca
     2941 attcgaggcg atgcgcgcgg ccggcgatct gctcgaatcg gccgacgtcc atggccatcg
     3001 ctacggtacg ccgcgcgccc cggtcgagaa cgtcctttcc gaaggccgcg acatgctgtt
     3061 cgacatcgac tggcagggcg cgcagcaatt gcgcgacgcg atgggcgccg acgtcgtcag
     3121 catcttcatc ctgccgccct cgatgcgcga attgaaggcg cggctcgacc gccgcgccga
     3181 ggacgccgcg gcgcagatcg cggcccggct cgacaatgcg cgcaaggaga tcgcgcgctg
     3241 gcggcagtac gattacgtgc tgatcaacga cgacctgcag cgcgcctatt ccgctgtggt
     3301 ctcgatcatc acggccgagc ggctgcggcg ccaccgcgtg gagaaaggcg tcgagacgtt
     3361 cgtcaaaggc ctgcttgacg actccgtcgg cggctcgtga agcgccggcg agccccttct
     3421 ccccgctgcg cagggctgtc cggggaaagt aagaggcgag tcgcatcgtc gtcgacgaag
     3481 tctcgtgaaa taacacgatt tcgagatttc cctcgttgag tttccggcgc cgagactcag
     3541 aaaataaaca tgcggacctc ggggctgctc acaaactctc gcggg
//
LOCUS       DATGJW010000434         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_292683, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000434 DATGJW010000000
VERSION     DATGJW010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_292683"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..680)
                     /locus_tag="VKU03_12960"
     CDS             complement(<1..680)
                     /locus_tag="VKU03_12960"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLJ72223.1"
                     /translation="MMAPIDLLSFQLYSARNFPPLWTQLATLSALGYRRVEPYGGLYA
                     ALDDLAAALQRHGLAAPSGHFDLDLLERDFERALKIARKLHVELVIAPFLPPPQRPND
                     TDGWRAFGARLAAIAAKFDAEGIGFGWHNHDFEMRALPDGSYPIDRLLSASDKIKWQA
                     DIGWIARASQDPLRWIARFAGRIGALHVKDLAKPGENEKEEGWADPGAGTLDLKAIVS
                     AGIAAGAK"
     gene            complement(788..1723)
                     /locus_tag="VKU03_12965"
     CDS             complement(788..1723)
                     /locus_tag="VKU03_12965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extensin family protein"
                     /protein_id="HLJ72224.1"
                     /translation="MPWLLGSCALLSLVGCGFYERAERPAWRNQAEEACLASHRVQPS
                     AFITPVNEIDGPGICGLYHPFKLSGLAGGTVSVEGPVTIDCSMIAALEDWLADVVQPR
                     AQARFGENVVGLTAFGAYSCRSIDNMAGARLSEHAFGNAIDVSGFRLADGRLISIVKE
                     WKRADSQESAFLREVHAGACQHFTTVLGPGADVFHYNHFHLDLAMHGTTNTGPRRYCK
                     PRPSPDLLPAPPPTDNLPPAPDVDEPQDIARMDQPADSAPIDLHSPTGALPPPARAYV
                     APPTRPPAYAPMTPRPPASIEGLLQDGDVTSSIAK"
     gene            1931..>2521
                     /locus_tag="VKU03_12970"
     CDS             1931..>2521
                     /locus_tag="VKU03_12970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1007 family protein"
                     /protein_id="HLJ72225.1"
                     /translation="MTSAAIFAVFLLPGAAAAHPHVWVTMHSEIAFSPGGLVSAIIHD
                     WTFDEMYSAFATQGLGKPGQLVTRDDFAPLAKQNAGSLAQIGYFTVLKIDGKQVDFGN
                     VTDYWMEERPDHLVAFHVVLPLKTPERPGKFVTMMVSDPEFFIDFEFDSKDPVKLDNP
                     PKGCSLSITHPAPLDVADSKKLNESFFTNLSPGAGFG"
BASE COUNT          449 a          814 c          830 g          428 t
ORIGIN      
        1 aatttcgcgc ccgccgcaat tcccgccgag acgatcgcct tcaagtcaag cgttccggcg
       61 ccgggatcgg cccagccttc ctctttctcg ttctcgcccg gcttggcgag gtctttgaca
      121 tgcagcgccc cgatccggcc ggcgaaacgc gcgatccagc gcaacgggtc ttggctggcc
      181 cgcgcgatcc agccgatatc ggcctgccat ttgatcttgt cgctcgccga caacagccga
      241 tcgatcgggt acgagccgtc cggcagcgcg cgcatttcga aatcgtgatt gtgccagccg
      301 aagccgattc cctcggcatc gaacttcgcc gcgatcgcgg cgagacgggc gccgaaagcg
      361 cgccatccgt ccgtatcgtt cgggcgctgc ggcggcggca ggaacggcgc gatcacgagc
      421 tccacgtgca gcttgcgcgc aatctttagc gcgcgctcga aatcgcgttc aagaagatcg
      481 agatcgaaat ggccgctcgg cgcggcgagg ccgtggcgct gcaaggccgc cgcgagatcg
      541 tcgagcgcgg catagagtcc gccgtacggc tcgacgcggc ggtagccaag cgcgctgagc
      601 gtcgcgagtt gcgtccatag cggcggaaaa ttgcgcgccg aatagagttg aaaggacaga
      661 agatcgatcg gagccatcat gcctcaaagc catgtcatcc ccgcgcaagc ggaatccagt
      721 acggcgtcct caggcgcgga ggcttctcga ttcccgcttt cgctggaatg acagtgacgc
      781 ttttatctca ctttgcaatc gaggacgtca catcgccgtc ctgcaacagc ccctcgatcg
      841 aggcgggcgg ccgcggcgtc atcggcgcat aggccggcgg gcgggtcggc ggcgcgacat
      901 aggcgcgcgc cggcggcggc aaggcgcctg tcgggctgtg caggtcgatc ggcgccgagt
      961 cggccggctg gtccatgcgc gcaatgtcct gcggctcgtc cacgtccggc gccggcggca
     1021 gattgtcggt cgggggcggc gccggcagca aatcggggct cggcctcggc ttgcaatagc
     1081 ggcgcgggcc ggtattggtc gtcccgtgca tggcgagatc gagatggaag tgattgtagt
     1141 gaaacacgtc cgcgccgggc ccgagcacgg tggtgaaatg ctggcaagcg ccggcatgaa
     1201 cctcgcgcag gaaagccgat tcctggctgt cggcgcgctt ccattccttg acgatgctga
     1261 tcaggcgccc gtcggccagc ctgaagccgc tgacatcgat ggcgttgccg aaggcgtgct
     1321 ctgagagccg cgcgcccgcc atattgtcga tgctgcggca gctataggcg ccgaaggcgg
     1381 tgaggccgac gacgttctcg ccgaagcgcg cctgggcgcg cggctggacg acatcagcga
     1441 gccaatcctc caaggcggcg atcatcgagc aatcgatcgt caccggcccc tcgacgctga
     1501 ccgtcccccc ggcaaggccg gaaagcttga acgggtgata aaggccgcaa atgccgggcc
     1561 cgtcgatctc gttgaccggc gtgataaagg ccgaaggctg aaccctgtgg ctggcgaggc
     1621 aggcttcctc ggcctggttg cgccaggccg gacgctcggc ccgctcgtaa aagccgcagc
     1681 cgacgagcga gagcagggcg caagagccca ggagccatgg caatacgcga cgcgccatct
     1741 ccacagcaat gcgcgcgtcc gattaataaa ggttgaaata tcgggcaatt cgcgcctcgg
     1801 gctccgaggc ggcgcgcagc gggccttttt cacgccaaag ttcggggtca tcagcggctg
     1861 acgccgttcg cgacgtgacg cgaagaacga cggggagcgg acggaaatgg ggcgaaatcg
     1921 gcgcggctca atgacaagcg ccgcgatttt cgccgttttt ctcctgcccg gcgccgccgc
     1981 ggcccatcct catgtctggg tcaccatgca tagcgagatc gccttctcgc cgggcggatt
     2041 ggtgagcgcg atcatccatg actggacgtt cgacgaaatg tattcggcct tcgcgacgca
     2101 ggggctcggc aagccgggcc aactcgtcac ccgcgacgat ttcgcgccgc tcgccaagca
     2161 gaacgccggc tcgctggctc agatcggcta tttcaccgtg ctgaagatcg acggcaagca
     2221 ggtcgatttc ggcaatgtga ccgattattg gatggaggag cggcccgacc atctggtggc
     2281 gttccatgtc gtcctgccgc tgaagacgcc cgagcggccg ggcaaattcg tcacgatgat
     2341 ggtgtcggac ccggaatttt tcatcgactt cgagttcgac agcaaggatc cggtgaagct
     2401 cgacaacccg cccaagggct gctcgctgtc gatcacccat ccggcgccgc tcgatgtcgc
     2461 cgattccaag aagctcaacg aatccttctt caccaatctc tcgcccggcg ccggcttcgg
     2521 c
//
LOCUS       DATGJW010000435         2727 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_706092, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000435 DATGJW010000000
VERSION     DATGJW010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2727)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2727)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2727
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_706092"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..652)
                     /gene="cbiE"
                     /locus_tag="VKU03_12975"
     CDS             complement(<1..652)
                     /gene="cbiE"
                     /locus_tag="VKU03_12975"
                     /inference="COORDINATES: protein motif:HMM:TIGR02467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046025 - precorrin-6Y
                     C5,15-methyltransferase (decarboxylating) activity
                     [Evidence IEA];
                     GO_process: GO:0046140 - corrin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-6y C5,15-methyltransferase
                     (decarboxylating) subunit CbiE"
                     /protein_id="HLJ72226.1"
                     /translation="MNPASRERWLTIVGIGEDGLDGLSPAAASLLKQARFVIGGARHL
                     QMLKAATAAETMEWPSPFERGVDAIKARRGAPTCVLASGDPYLYGVGATLAAHIPPEE
                     TICLPAPSSLSLAASRLGWPLQDCDIVSLHGRPFERLIPHLRPKARLLVLSWDETTPE
                     KIARLLVARGLGKSRLIVMAALGGSRETVVERLACDFASENFDPLNLVAIEIDDAAA"
     gene            1108..1860
                     /locus_tag="VKU03_12980"
     CDS             1108..1860
                     /locus_tag="VKU03_12980"
                     /EC_number="1.3.1.106"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016994 - precorrin-6A reductase activity
                     [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalt-precorrin-6A reductase"
                     /protein_id="HLJ72227.1"
                     /translation="MRVLILGGSSEASRLAARLAEEKGFAPILSLAGRTRDPSPPPIP
                     YRIGGFGGAAGLAAYLSAERIDCLIDATHPFAERMSANAEEAAAATGTPLFVFTRPPW
                     TPGPGDRWIECSSAGEAAQALGSASRRVFLTVGRLQLAAFEAAPQHDYLIRTIDPPHP
                     APALPRWRLILARGPFGVEAETQLMRAEGIEILATKNSGGEATRAKLDAARALGLDVI
                     LIRRPPAARATTVYTIDAAMAFLRAERHRASP"
     gene            complement(1842..2606)
                     /gene="cobJ"
                     /locus_tag="VKU03_12985"
     CDS             complement(1842..2606)
                     /gene="cobJ"
                     /locus_tag="VKU03_12985"
                     /EC_number="2.1.1.131"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030789 - precorrin-3B
                     C17-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B C(17)-methyltransferase"
                     /protein_id="HLJ72228.1"
                     /translation="MTQGRLDIVGLGPGPREWMTLEALSLIEAATDLVGYRPYLDRLP
                     IAAGQRRHASDNRVEIERARMALDLALAGGRVAVVSGGDPGIFAMAAAVFEAVETGNP
                     RWRSLEIATHPGVSAMLAAAARLGAPLGHDFVALSLSDNLKPWSAIERRLKAAAEGDF
                     VMALYNPASAARPKPIHDAFALLRALKPAETIVAFARAIGRSDERIELASLGAADPAR
                     ADMSTLVLVGSSQTRRVERGQGGFWIYTPRGYGEAR"
     gene            complement(2603..>2727)
                     /locus_tag="VKU03_12990"
     CDS             complement(2603..>2727)
                     /locus_tag="VKU03_12990"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017484836.1"
                     /note="catalyzes the formation of precorrin-3 from
                     precorrin-2; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HLJ72229.1"
                     /translation="AIYVERGTMDGESVMRLADKSDDSAPYFSLILIPGEGRRP"
BASE COUNT          453 a          942 c          921 g          411 t
ORIGIN      
        1 ttgccgccgc atcgtcgatt tcgatcgcga cgagattgag cggatcaaag ttctcggacg
       61 caaaatcgca agccaggcgt tccacgacgg tttcgcgtga gccgccaagc gcagccatca
      121 caatcagccg agacttgccc aagccgcgcg caaccagcag gcgcgcgatc ttctcgggcg
      181 ttgtctcgtc ccaggagaga accaggagcc gcgccttcgg ccgcagatgg gggataaggc
      241 gctcgaaggg ccggccatgc agcgagacga tgtcgcaatc ctgcagcggc cagccgagcc
      301 gcgaagcggc gaggctgagc gaggacggcg ccggcaggca gatcgtctct tccggcggaa
      361 tatgagcggc gagcgtcgcg ccgacgccgt agaggtacgg atcgccagag gcgagcacgc
      421 aagtcggcgc gccgcgccgc gctttgatcg cgtcgacccc gcgctcgaag ggcgaaggcc
      481 attccatcgt ctcagcggcg gtcgccgcct tcagcatctg aagatggcgc gcgccgccga
      541 tcacgaaacg cgcttgcttg agaaggctag ccgcggcagg agagagcccg tcgagcccgt
      601 cctcgccgat tccgacgatc gtcagccagc gctcgcggct cgcggggttc atgggccgcg
      661 ctccgcgtcg tgacgcaaat cccaaatggg gcgccgccat cgacgcgcgg aagcgtcaac
      721 atcaagccgg cttcgccggt cgctgcgcct cgctccgcga atgttgactc ttccgcgcgc
      781 cgatggcctt tttgccatca agacattttg ctgctcgaaa cggccgaacg gccggtttcg
      841 gcagcgaaat atcttggtag ccaataagcc acgccgaccg agcgcagtca cccgccatag
      901 cgccgcacaa gaggaggtcc gttttatccg taccttggag gcgctatggc gggttgacgg
      961 cgatcgggcg gcgcggcgcc gcggcgttga aagcaacggc gccgatatcg tcgaggcgca
     1021 ggcaatctgg aaagcgagat tgacgtcaga gatcgttgca accttccccc ggacaagccc
     1081 gcaacgcgcg gcgacccaaa gagcacgatg cgcgttctga tcttgggcgg atcgagcgag
     1141 gcgagccgcc tcgccgcgcg gctcgcggaa gaaaaaggct tcgcgccgat tctgtcgctc
     1201 gccggacgga cccgcgatcc gtctccgccg ccgattccct atcgcatcgg cggttttggc
     1261 ggggccgccg ggctcgccgc ctatctgagc gccgagcgca tcgattgtct catcgacgcc
     1321 actcaccctt tcgccgagcg catgtccgcg aacgccgaag aagccgccgc cgcgacggga
     1381 acgccgcttt tcgtcttcac ccgtcctcct tggactccgg gtcccggcga ccgatggatc
     1441 gaatgctcga gcgccggcga ggcggcgcaa gccctcggct ctgcgtcgcg ccgggttttc
     1501 ttgacggtcg gccggctgca attggccgcc ttcgaggcgg cgccgcagca cgattatctg
     1561 atccggacga tcgatccgcc ccacccggcg ccggccttgc cgcgctggcg gctgatcctg
     1621 gcgcgcgggc ctttcggcgt cgaggccgag acgcaattga tgcgcgccga gggaatcgag
     1681 attctggcga ccaagaatag cggcggcgag gcgacccgcg ccaagctcga cgcggcgcgc
     1741 gctctggggc tcgacgtgat cttgatccgc cgcccgccgg cggcgcgcgc aacgactgtc
     1801 tacacgatcg acgcggcgat ggccttcctg cgcgccgagc gtcatcgcgc ctctccgtag
     1861 cctcgcggcg tatagatcca gaagccgcct tgcccgcgct cgacacgccg cgtctggctg
     1921 gagccgacca ggaccagcgt gctcatatcg gcccgagcgg gatcggccgc gccgaggctg
     1981 gccagttcaa tgcgctcgtc ggagcggccg atcgcccggg cgaaggcgac gatcgtctcg
     2041 gcgggcttga gcgcgcgcag cagcgcgaag gcgtcatgga tcggcttcgg ccgcgcggcc
     2101 gaggccggat tgtacaaagc catcacgaag tcgccctcgg cggcggcctt gaggcggcgc
     2161 tcgatcgcgc tccatggttt gagattgtcc gacagcgaca acgcgacgaa atcgtggccg
     2221 agcggcgcgc cgagccgcgc cgccgcggcg agcatggccg agacgcccgg atgcgtcgca
     2281 atctctagcg agcgccagcg cggattgccg gtctccaccg cctcaaatac ggccgccgcc
     2341 atggcgaaaa ttcccggatc gccgcccgag accacggcga cgcggccgcc ggcaagagcc
     2401 aagtcgagcg ccatgcgggc gcgctcgatt tcgacgcgat tgtcgctggc gtgccgccgc
     2461 tgccccgccg cgatcggcag gcgatcgaga taggggcgat agccgacgag atcggtcgcc
     2521 gcctcgatca gcgacagcgc ttcgagcgtc atccattcgc gcgggcccgg cccgaggccg
     2581 acaatgtcga ggcggccctg ggtcatggcc gccgtccctc gccggggatg aggatcagcg
     2641 aaaaataggg cgcgctgtca tcgcttttgt cggcgagccg catcacgctc tcgccgtcca
     2701 tcgtgccgcg ctcgacatag atggcgc
//
LOCUS       DATGJW010000436         8416 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1533177, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000436 DATGJW010000000
VERSION     DATGJW010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8416)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8416)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8416
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533177"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(31..1320)
                     /locus_tag="VKU03_12995"
     CDS             complement(31..1320)
                     /locus_tag="VKU03_12995"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ72230.1"
                     /translation="MESTLKDRPRIAALVDLPRATEAGGHVKYWERIAHAAAEAADFP
                     FDLTVYFSGDAPDEVLSPAVRLRHLRPIFSTARLKFLPYVPDHTDLAPYHPALARALA
                     DCDLIHTTDGFFAFAKTAERVARAKGKPLTTSFHTDTPSYARIFTRQTIETLFGRWPT
                     LSRALIETWRAPERQSAKTERRLEQHLSAATRAFATRREDRRLAERVLGAGRVDALRL
                     GVDKALFDPSRRDRDALRRDYAIPSGGVAALFVGRVDVGKNVPILMEAAARAIAAGAP
                     LHLIVAGLGPLRDALAEKLEGHVSLPGFVPPGELARLYASVDFTVLPSEVEIGGMAPG
                     EAMAAGSPLLVARGGGAADIYGETPAIWIVEAGVDAWTAALIEITKDARRRAPMRQAA
                     LDDSARSLASWTDVVREDLVPGWRAALTSAGAARERR"
     gene            1530..2675
                     /gene="hpnH"
                     /locus_tag="VKU03_13000"
     CDS             1530..2675
                     /gene="hpnH"
                     /locus_tag="VKU03_13000"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017486661.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HLJ72231.1"
                     /translation="MGIPLRQMAGVGAYVVRQKLKGVKRYPLVLMLEPLFRCNLACAG
                     CGKIDYPDKILNQRLSVADSLQAMDECGAPVVVIAGGEPLLHRELDQIVQGALARGKY
                     VTVCTNALLLEKKLDQFKPHPRFNWSIHLDGDQEMHDRSVCQSGVYDRAVDALKAAKA
                     KGFRCNINCTLFDGADPSRVAAFLDETKRLGVEGVTISPGYAYERAPDQKHFLNRTRT
                     KQLFRDIFAKGRGKKWAFYQSSLFLDFLAGNQTYHCTPWGNPTRTVFGWQRPCYLLGE
                     GYAKTFKELMESTDWDSYGVGAYEKCADCMVHSGFEATAVNDALAHPMKLLKLAVSGV
                     RTDGPMAPDISLSKQRPAEFVFSRHVETKLAEIDASESRTKKRARVG"
     gene            complement(2732..3991)
                     /gene="hpnE"
                     /locus_tag="VKU03_13005"
     CDS             complement(2732..3991)
                     /gene="hpnE"
                     /locus_tag="VKU03_13005"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592218.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HLJ72232.1"
                     /translation="MRRPTVHIIGAGVAGLAAAVKLSSAPASIVVHEGARQAGGRCRS
                     FFDETINATIDNGNHLLLSGNRSALAYLDAIGARKELKGPDRCEIDFADMATNERWTL
                     RPNEGLLPWWIFVEGRRTPGVKVAEYFGAARLLWAARNARFSDAVSCSGRLYERLWRP
                     FFLAALNTDPKDGSAALALTLLRETFARGGRACHPLVAAHGLSRAFVDPALRLLQKRG
                     AQIRFERRLRGIEFTGDRAASLDFEHDSVAVEEGDVVILATPSHIAASLVPDLSTPQK
                     TNAILNGHFHIAPPKGTPPIIGVVNGTVEWIFAYSDRLSITISDAERFMDTPREKLAA
                     DLWRDVAALTNLPDALPSWRVIKEKRATFAATPEENARRPATATRWRNFLLAGDYVNT
                     GLPATIEGAIRSGDTAAALALGQIEAR"
     gene            complement(3991..4809)
                     /gene="hpnD"
                     /locus_tag="VKU03_13010"
     CDS             complement(3991..4809)
                     /gene="hpnD"
                     /locus_tag="VKU03_13010"
                     /EC_number="2.5.1.103"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177155.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016767 - geranylgeranyl-diphosphate
                     geranylgeranyltransferase activity [Evidence IEA];
                     GO_process: GO:0016117 - carotenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="presqualene diphosphate synthase HpnD"
                     /protein_id="HLJ72233.1"
                     /translation="MSEAAVPAPRRSSFYTAMRVLPREKREAMYEIYNFCRAVDDIAD
                     DAARPRADRHVALQAWRDDIDKLYAGSVPERLASLAKAIRDYDLKRENFLAIIDGMDM
                     DVATDIRAPDRATFDIYCDRVACAVGRLSTQVFGLDEKTGAALADHLGRALQVTNILR
                     DLDEDAGFGRLYLPRDALDRAGIASSDPQAVLADPALDIAAQSLVAEARTRFEKANEV
                     MATSSRAKTRAPRLMAIVYRKILDQVAARGFAPPRAPVSVSRAGLIFAALRGFF"
     gene            complement(4806..5675)
                     /gene="hpnC"
                     /locus_tag="VKU03_13015"
     CDS             complement(4806..5675)
                     /gene="hpnC"
                     /locus_tag="VKU03_13015"
                     /EC_number="2.5.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004311 - farnesyltranstransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="squalene synthase HpnC"
                     /protein_id="HLJ72234.1"
                     /translation="MTENISPHRTENSENFPVASRLIAPRHRAPIIAFYRFVRAADDI
                     ADHPTMTPEAKLAGLDRMAAALNGEREDAPAGIALRVALAARDLTPRHALDLLAAFRL
                     DVTKKRYADWNDLMGYCALSAMPVGRYVLDVHGESAALWPASDAICASLQVINHLQDC
                     GEDFAQLDRVYIPEETFSRHGARVEDLGAKSASPALRAVVSDLARRSDALLDQGAALV
                     GSIKDFRLSLEIGAIVRLARANCARLQHRDPLSERVHPSKAGFAALGLLGAMEGVIAR
                     IGGGHRHATGPAT"
     gene            complement(5778..6785)
                     /gene="hpnA"
                     /locus_tag="VKU03_13020"
     CDS             complement(5778..6785)
                     /gene="hpnA"
                     /locus_tag="VKU03_13020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013949379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hopanoid-associated sugar epimerase"
                     /protein_id="HLJ72235.1"
                     /translation="MKVAIEAQDLIVVTGASGFVGSAVVEKLLAERARIRAIVRPTSA
                     RIPAPRDGVEIALADMRDTALMKGAMRGARFLFHVAADYRLWAKNPREVVEANVEGTR
                     AVMAAAADAGVERVVYTSSVATLAPAADAPVDETAVLTERHAVGAYKRSKIAAERLVE
                     ILIADGLPAVIVNPSTPIGPRDGRPTPTGRILIEAAQGRMPAYVDTGLNLVHVDDVAA
                     GHVAALKIGRIGERYILGGDDVTLGRMLADIAALVGRPPPQVKLPRAPLYPLAVAAEA
                     VAQITGREPFLTLDGLRMAKKKMFFTSAKAERELAYRHRPYHEAIEDALDWFRQAGML
                     K"
     gene            7000..7437
                     /locus_tag="VKU03_13025"
     CDS             7000..7437
                     /locus_tag="VKU03_13025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020187156.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HLJ72236.1"
                     /translation="MGGLMSSWQIWALLSAAFAALTAIFGKVGVEKVGADLATFIRTI
                     VILGFAALIVTASGQWRDAGAISARSGIFLVLSGLATGASWLCYYRALQIGDAARVAP
                     IDKLSVVLVAVFGVSFLSERLSGPNWLGVALIAAGAILVAYRG"
     gene            complement(7589..8158)
                     /locus_tag="VKU03_13030"
     CDS             complement(7589..8158)
                     /locus_tag="VKU03_13030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72237.1"
                     /translation="MKTRHVAALALLSVAAALIAFVSIIGLDGLAYVLIALEPARHQN
                     AIEAGNLLGTWAVDCGRPPSPLNRHIVISPATGIDGFIAYRSSEGARGDTFKVLSADG
                     RMEFFYTRLTDELTPGDGTVGYVELTQLADTGQKTLVTLAMAIGDKPGEWGKLTVAAW
                     PRADGTFEIRNGIRLADQTAYGWLKRCGT"
BASE COUNT         1525 a         2706 c         2733 g         1452 t
ORIGIN      
        1 cgagaatgag gatacgcctg ccgaacatat ttaacgccgc tcccgtgcgg cgccggcgct
       61 tgtgagcgcg gcgcgccagc cggggacaag atcttctctg accacatcgg tccagctcgc
      121 gaggctccgt gcgctgtcgt caagcgcggc ttggcgcatc ggcgcgcggc ggcgcgcgtc
      181 cttcgttatt tcgatcagcg ccgccgtcca ggcgtcgacg ccggcctcga cgatccagat
      241 cgccggcgtt tcgccataga tatcggcggc gccgccgccg cgcgcgacca gcagcggcga
      301 acccgccgcc atcgcctccc ccggcgccat gccgccgatc tccacttccg agggcagcac
      361 ggtgaaatcg acgctggcgt agagccgcgc cagttcgccg ggaggtacga agccgggcag
      421 gctgacatgc ccttcgagtt tttcggccaa ggcgtcacgg agcggaccca atccggcgac
      481 aatgagatga agcggcgcgc cggcggcgat cgcccgcgcc gccgcctcca tcaggatagg
      541 tacgttcttg ccgacatcga cgcggccgac gaacaaggcg gccacgccgc cggagggtat
      601 cgcgtaatct cgccgcaacg cgtcgcggtc gcgccgggag ggatcgaaga gcgccttatc
      661 gacgccaaga cgaagggcgt cgacacgccc agcgccaagg acccgttcgg cgagacgccg
      721 atcctcgcgg cgcgtcgcaa aagcccgcgt cgcggcgctg agatgctgtt cgagccggcg
      781 ctccgttttg gcgctttggc gctccggcgc gcgccaggtc tcgatcagcg cacggctaag
      841 cgtcggccag cggccgaaaa gggtctcgat cgtctggcgg gtgaaaatgc gggcgtaact
      901 cggcgtgtcg gtgtggaaag aggtggtcag cggcttgccc ttggcgcgag cgaccctttc
      961 cgccgtcttg gcgaaggcga aaaacccgtc ggtcgtatgg atgagatcac aatccgcaag
     1021 ggcccgggcc aaagccggat gataaggcgc aagatcggtg tggtccggca catagggcag
     1081 aaatttcagg cgcgcggtgc tgaagatcgg cctcagatgg cgcagccgca ccgccggcga
     1141 caagacctcg tcgggcgcgt cgcccgagaa ataaaccgtc agatcgaagg gaaaatccgc
     1201 cgcttcggcc gccgcatggg cgatccgctc ccaatatttg acatggccgc cggcctcggt
     1261 cgcgcgcggc aggtcgacga gcgcggcgat ccgcgggcgg tctttaaggg ttgattccaa
     1321 agccaggcca aattttatcg cgtcccgacc tagcagggcg gccccctcgt cgccaaatgc
     1381 ggcttttagg ggagcgacgg gctgcgttgg cggaattcaa gccaagcggg accggcaatt
     1441 cccttgcggc cgcaattgct gtaggaagaa cgccaactcg gccagccctc gcctgccgtt
     1501 aagctaatcc gctgaaaagg atgaatcatt tgggcattcc gctgcgacaa atggccggcg
     1561 tcggcgcata tgtggttcgc cagaagctga aaggcgtcaa gcgctatccg ctggtcttga
     1621 tgctggagcc gctgtttcgc tgcaatctcg cctgcgcggg ctgcggcaaa atcgactacc
     1681 ccgacaaaat cctcaaccag cgcctttccg tcgccgacag cctccaggcg atggacgaat
     1741 gcggcgcgcc cgtcgtcgtc atcgccggtg gcgagccgct gctgcatcgt gagctcgacc
     1801 agatcgtcca aggcgctctc gcccgcggca aatacgtgac ggtttgcacg aatgcgctgc
     1861 tgctcgagaa gaagctcgat cagttcaagc cgcatccgcg cttcaactgg tcgatccatc
     1921 tcgacggaga ccaggaaatg cacgatcgct cggtctgcca gagcggcgtc tatgaccggg
     1981 ccgtcgacgc gctgaaagcc gccaaggcca aaggctttcg ctgcaacatc aattgcacgc
     2041 tgttcgacgg cgccgatccg tcgcgcgtcg cggctttcct cgacgagacc aagcgcctcg
     2101 gcgtcgaagg cgtcacgatc tcgcccggct acgcctatga gcgtgcgccc gaccagaagc
     2161 acttcctcaa ccgtacccgc accaagcaat tgttccgcga tattttcgct aagggtcgcg
     2221 gcaagaaatg ggctttctac cagtcctcgc tgttcctgga cttcctcgcc ggcaaccaga
     2281 cttatcattg cacgccctgg ggcaatccga cacgtaccgt cttcggctgg cagcggcctt
     2341 gctatctgct cggtgaaggc tacgccaaga cgttcaagga actgatggag tcgacggatt
     2401 gggattccta cggcgtcggc gcctatgaga aatgcgccga ttgcatggtg catagcggct
     2461 tcgaggcgac cgccgtcaat gacgcgctcg cccatccgat gaagctgctg aagctcgccg
     2521 tctccggcgt gcgcaccgac ggcccgatgg cgccggatat ttcgctcagc aagcagcggc
     2581 cggcggaatt cgtcttctcc cggcatgtcg agacgaagct cgccgagatc gacgcgagcg
     2641 agtcccgcac caagaagcgg gcgcgcgtcg ggtagcttcc cctcaccccg accctctccc
     2701 gcaagcggga gagggggcgc gctgaggtaa ttcaccgcgc ctcgatctga cccagggcaa
     2761 gcgccgccgc ggtatcgccg gagcgtatcg cgccttcgat cgtcgccggc aggccggtgt
     2821 tcacataatc gccggcgagc aggaaattgc gccagcgggt cgccgtggcc ggccgacgcg
     2881 cgttctcttc gggcgtcgcg gcgaaagtcg cgcgcttttc cttgatgacc cgccaggacg
     2941 gcaaggcgtc gggcaaattg gtcagcgcgg cgacatcgcg ccagagatcg gcggcgagtt
     3001 tttcgcgcgg cgtatccatg aaacgttcgg cgtcgctaat cgtgatcgac aggcgatccg
     3061 aataggcgaa aatccattcg acggtgccat tgacgacgcc gatgatcggc ggcgtacctt
     3121 tgggcggcgc gatgtggaaa tggccgttga gtatcgcgtt cgtcttctgt ggcgtcgaaa
     3181 gatccggcac gagcgaagca gcgatatgcg aaggcgtcgc caggatgacg acgtcgccct
     3241 cctcgacagc aaccgaatcg tgttcgaaat cgaggcttgc ggcgcgatcg ccggtaaatt
     3301 cgatcccgcg caggcgccgt tcgaaacgga tttgcgcgcc gcgtttttgc agcaggcgca
     3361 gggcggggtc gacaaaggcg cgcgacaggc catgcgccgc gacaagagga tggcaagcgc
     3421 ggccaccgcg cgcgaaagtc tcgcgcaaga gggtcagcgc cagggccgcc gaaccgtcct
     3481 tggggtccgt attcagagcc gccaggaaga acggacgcca gagcctctcg tagagccgtc
     3541 ccgaacagga gaccgcgtcg gaaaatcgcg cgttgcgcgc cgcccacagc aggcgcgccg
     3601 cgccgaaata ttcagcgacc tttacgccgg gcgtgcggcg tccctcgaca aaaatccacc
     3661 agggcagcag cccctcgttc ggacgcaagg tccagcgttc gttcgtcgcc atgtcggcga
     3721 aatcgatctc gcagcggtcc gggcccttga gttctttgcg cgcgccgatg gcgtcgagat
     3781 aggcgagcgc cgaccgattg ccggacaaga gcaaatggtt gccattgtcg atcgtcgcat
     3841 tgatcgtctc gtcgaagaaa gaacggcaac ggccgccggc ctggcgcgcg ccttcatgaa
     3901 cgacgatcga ggccggcgct gaggagagct tcaccgcggc ggcgaggccc gcgacgcctg
     3961 cgccgataat atggacggtc gggcggcgca ttaaaaaaat ccacgcaagg cggcgaagat
     4021 cagaccggcg cgcgagacgc tgaccggcgc gcgcggcggc gcaaagccgc gcgccgcgac
     4081 ctggtcgaga atcttacgat agacgatcgc catgaggcgg ggcgcgcgcg tcttggcgcg
     4141 cgaacttgtc gccatcactt cattggcctt ttcaaagcgc gtacgcgcct cggcgaccag
     4201 cgattgcgcg gcgatatcaa gcgccggatc ggcgaggacc gcctgagggt cgctgctcgc
     4261 aatgccggcg cgatccagcg cgtcgcgcgg cagatagagg cggccgaagc cggcgtcctc
     4321 gtcgagatct cgcaaaatat tggtcacctg caaggcgcgg ccgagatgat cggcgagcgc
     4381 cgcgccggtc ttttcatcga gcccaaagac ctgcgtcgac aagcgcccga ccgcgcaggc
     4441 gacgcgatcg cagtagatat cgaatgtcgc gcgatccggc gcacgaatat cggtcgccac
     4501 atccatatcc atgccatcga tgatcgccaa aaaattttct cgtttgagat catagtcacg
     4561 gatcgcttta gcgaggctgg cgagccgctc gggaaccgag ccggcgtaga gcttgtcgat
     4621 atcgtctcgc caagcctgca acgcgacatg acggtcggcg cgcggccgcg cggcgtcatc
     4681 ggcgatatcg tcgacggcgc ggcagaaatt gtagatttcg tacatcgctt cgcgcttctc
     4741 gcgcggaagc acgcgcatcg ccgtatagaa ggaactgcgg cgcggcgccg gaacggctgc
     4801 ctcgctcatg tcgcgggacc cgtggcgtga cgatgaccgc cgccgatacg cgcgatgacg
     4861 ccttccatcg cgccaagaag gccaagcgcg gcgaagcccg ccttgctggg atggacgcgc
     4921 tcgctcagcg gatcgcgatg ctgaagccgc gcgcaattgg cgcgcgccaa tcggacgatc
     4981 gcgccgattt cgagggacag gcggaaatcc tttatcgacc cgaccaaagc ggcgccttga
     5041 tcgagcaacg cgtccgaccg gcgcgcgaga tcgctgacca cggcgcgcag ggctggcgat
     5101 gcgcttttcg cgccgaggtc ttcgaccctg gcgccgtggc gggagaaagt ctcttccgga
     5161 atgtagacgc ggtcgagctg cgcaaaatcc tcgccgcaat cctgcagatg attgatcact
     5221 tgcaagctcg cgcagatcgc atcgctcgcc ggccacagag cggcgctttc gccgtgaacg
     5281 tcgagcacgt agcgcccgac cggcatggcc gagagcgcac aatagcccat gagatcattc
     5341 caatcggcat agcgtttttt cgtgacgtcg agacggaagg cggcgagaag atcaagggca
     5401 tgacgcggcg tcaaatcgcg cgcggcgaga gcgacgcgca gagcgattcc cgccggcgcg
     5461 tcctcgcgct cgccattcag cgccgcggcc atgcgatcga gtccggcgag cttggcctcc
     5521 ggcgtcatag ttgggtggtc ggcgatgtcg tcggcggcgc ggacgaagcg ataaaaggcg
     5581 ataatcggag cgcggtggcg cggcgcgatc aggcgcgaag cgaccggaaa attctcgcta
     5641 ttctcggttc gatgcggaga gatattttca gtcacggtcc caaacgtcga tgttgcaggc
     5701 gaggttcgtc ggattcactt cgttgattga gcataggccc ttcgcgttaa agtacgtcaa
     5761 tcgccaattc ggccttccta tttcagcatc ccggcctgtc tgaaccaatc gagcgcgtcc
     5821 tcgatcgcct cgtggtacgg acgatgccta taggccaatt cgcgctcggc ttttgccgag
     5881 gtgaagaaca ttttcttctt ggccatgcgc agcccatcca gggtcaggaa cggctcgcgg
     5941 ccggtgatct gggcgacggc ctccgccgcg acggccagcg gataaagcgg cgcccgcggc
     6001 aatttgacct gcggcggcgg ccgaccgacc aaagcggcaa tatcggcgag catgcgccca
     6061 agcgtcacat cgtcgccgcc gagaatatag cgttcgccga tccggccgat tttcaacgcc
     6121 gcgacatggc ccgccgcgac atcgtcgaca tggacgagat tgaggccggt gtcgacatag
     6181 gctggcatgc ggccttgcgc ggcttcgatc aggatgcggc cggttggcgt cggccggccg
     6241 tcgcgtggac cgatcggcgt cgagggattg acgatgacgg cgggcaggcc atcggcgatc
     6301 aggatttcga caagacgttc ggcggcgatt ttgctgcgct tgtaagcgcc gaccgcgtgt
     6361 ctttcggtca ggaccgcggt ctcgtcgacc ggcgcgtcgg cggcgggcgc gagggttgcg
     6421 acgctgctcg tatagaccac ccgctcaacg ccggcgtcgg cggccgccgc catgaccgcg
     6481 cgcgttccct cgacattggc ctcgacgacc tcgcggggat tcttggccca cagccgatag
     6541 tcggcggcga catggaaaag aaaacgcgcg ccgcgcatcg cgcctttcat gagagccgtg
     6601 tcgcgcatgt cggcgagcgc gatttcgacg ccatcgcgcg gcgcgggaat gcgtgcgctg
     6661 gttggccgca cgatcgcgcg tatgcgggct ctctccgcga ggagcttttc gaccacggcg
     6721 gagccgacaa agcccgaggc gccggtgacg acgataaggt cctgcgcttc gatcgcgact
     6781 ttcatgagcg ccgaaccgca acacggcctt ccccgcgccg gcgggctccg ccccgcctca
     6841 gccgtcgagc ccccctgccc cgctccgtat ctccccccgc ccggcgggga gtggaggcct
     6901 tagcgccgcg ccttaaaatc atcgcgatgg tttcttctcg attggccgcc tcaggagcga
     6961 ggtggataat caactttcat tagcaaagcg agggcgacga tggggggatt gatgtcttcc
     7021 tggcagatct gggcgctgct ttccgcggct ttcgccgccc tgaccgccat attcggcaag
     7081 gtcggcgtcg agaaagtcgg cgcggacctt gccactttca ttcgcacgat cgtgatcctg
     7141 ggctttgccg ccctgatcgt gaccgcgagc gggcaatggc gcgacgccgg cgcgatttcc
     7201 gcccggagcg gaatttttct ggttctgtca ggcctcgcca ccggcgcctc ctggctttgc
     7261 tattaccgcg ccttgcaaat cggcgacgcc gcccgcgtcg cgccgatcga taagctgagc
     7321 gtcgtgctgg tcgccgtctt cggcgtcagc tttctctcgg aaaggctgag cgggcccaat
     7381 tggctcggcg tggcgctgat tgcggcgggc gctattctgg tggcttatcg ggggtgatgt
     7441 tcagacgcca aatcctcgaa cagggtcacg gcgtctgccg agcgagttcg cgcagggccg
     7501 ctcacaggcg accgtcggga cgcttggcgc cgcgaccgag ccagtctcca gaccatgcgg
     7561 gggatttgcg caatccggcg cggcgcggtc acgttccgca ccgcttcagc caaccatagg
     7621 cggtctgatc ggcgagccgg attccgttcc gaatttcaaa cgttccatcc gcgcgcggcc
     7681 aggcggcaac ggtcaatttg ccccattcgc ctggcttgtc gccgatggcc atcgccaggg
     7741 taaccagggt tttctgtccc gtgtccgcca attgtgtgag ctcgacgtaa cccaccgtcc
     7801 catcacccgg ggtcagctcg tcggtcagcc gtgtgtagaa gaattccatg cgcccgtccg
     7861 cggacagcac tttaaacgtg tcaccgcgtg ccccttcgct cgaccgatac gcgatgaatc
     7921 cgtcgatgcc ggtggcgggc gagatcacaa tgtgcctgtt gagcggcgat ggcggccggc
     7981 cgcaatcaac ggcccatgtc ccaaggagat ttccggcctc aatggcgttc tggtgacgag
     8041 caggctccag agcgatgagc acataggcga ggccgtcgag cccaataatg cttacgaagg
     8101 cgatcagcgc cgccgcgacg gagaggagcg ccagggctgc gacatgacgc gtcttcattt
     8161 ggtggcttca tgaaccggat caccgtcgct gcgccgacag cacgccgacg tcgccacgca
     8221 tgatcacagg atgggcaagg ttcttttacc cctcactgcg ttcaccgcac aactgacgct
     8281 cggccccgat cagaagaaac gcgttatcca ggccgggaag acaacaagac acatggtttg
     8341 tgtttctgtg aaactctttt gagggtcatg accgcaacgt cgcccgtcgc gatcttaccg
     8401 atcaagacgg cgttgg
//
LOCUS       DATGJW010000437        13261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_914901, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000437 DATGJW010000000
VERSION     DATGJW010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 13261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13261
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_914901"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1440)
                     /locus_tag="VKU03_13035"
     CDS             complement(<1..1440)
                     /locus_tag="VKU03_13035"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat-containing
                     glycosyltransferase family protein"
                     /protein_id="HLJ72238.1"
                     /translation="MARLSAAHAALRAWERALTLQERSAEAFYRHGAALWNAGRQQEA
                     IAHLDAALRENPDYPEALCMGGFILGQQGKTDAALRFYARALALKTDFTIAWSNSGKL
                     LFDLGRYEEAASAFARVVALAPQDADGWNAYAGALRELGRMEESAEAARKALKLRPSF
                     AEAALNLGNALLKRDRAEEALALYRKAKALKPDFAAAWCGEALALRALDRLDEALAAF
                     EGAERLGSNGAIGGKGCLYLTLGDFERGWEGYEARWLNGKSLKEALGARFPVWTGALT
                     KGAKVLVMKDHGLGDVIQFFRYVALMAEAGVETTLFCPPSLHRLLSSFPGVRFIGAMD
                     EAPYDAQIAISSLPRAFGTRLDTIPTPIPYLHAEPALERKWAARIGEAGFKIGIVWQG
                     NANPEADMARAMPLSSFAPLASLPDIRLISLQKGFGTEQIAALPPTMTVETLGEDFDS
                     GPDAFVDTAAAMMALDLVISCDTSVAHLAG"
     gene            complement(1520..2407)
                     /locus_tag="VKU03_13040"
     CDS             complement(1520..2407)
                     /locus_tag="VKU03_13040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177013.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1217 domain-containing protein"
                     /protein_id="HLJ72239.1"
                     /translation="MTTTTAAYLQVENNLSRYQSMTAKEPAVKTASAYYAANIGKVTS
                     ISDFVSNYRLLSYALDAYGLGDQINSKALITKILEGGVSNPKSLANTLQGGKWKAFAA
                     AFDFVGQGAASVSSKTAIQTTTNNYVEQQLESDQGEEDPGVQLALYFQRVAPSVTSAY
                     GILADKNLLEVVQTIFGLPPASNANNIDAEAKQIIKLMPLSDLQNPTKLKQLVERFTA
                     MYDVEYGPTSGSTSSLTVSGGNNASASAGSAASSILSGVITSNGQAISAALNSSSDSP
                     TPLFSNSFLQSLQKFRLGG"
     gene            2578..3651
                     /locus_tag="VKU03_13045"
     CDS             2578..3651
                     /locus_tag="VKU03_13045"
                     /EC_number="4.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009975 - cyclase activity [Evidence IEA];
                     GO_function: GO:0016849 - phosphorus-oxygen lyase activity
                     [Evidence IEA];
                     GO_function: GO:0032555 - purine ribonucleotide binding
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ72240.1"
                     /translation="MPSFLRLPSRLQVILWFTASAAVVGALYAHFHAVMEGDRLLSLS
                     GVPRGAMTGAIIASILISFEISVVAEPLGARLRQARFLLHVAVKTFFYLIVILVALEL
                     GAWALPAAGERGVELRDVAFSLAASFVFVFLFDINRLLGQNVLLNFVTGRYHQPRLEE
                     RVFLFIDMEGSTGFAERLGPVAFHRLLNRFVVDLTGPIVAARGEVYRYVGDELIATWR
                     LNEGTADARCVAACFAAMDRFALLASAYRREFGAAVNFRAGLHCGPVVTGEMGSVKKE
                     IVFLGDTMNTAARIQEFCRQTGDRVLASADLLDRLALPPGVVKRPLGDLRLRGKESQV
                     ALYALTKTAPGRRQAREGSVAEA"
     gene            complement(3660..3980)
                     /locus_tag="VKU03_13050"
     CDS             complement(3660..3980)
                     /locus_tag="VKU03_13050"
                     /inference="COORDINATES: protein motif:HMM:NF017922.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gamma-butyrobetaine hydroxylase-like
                     domain-containing protein"
                     /protein_id="HLJ72241.1"
                     /translation="MTENADEAWPTEIRLTDEGRKLTIALDSGESFTLSSSYLRKMTP
                     SVEAKRLTEAERERLIGSRPARITAIEPIGNYAIRPTFDDGHSTGIFSWTYLAELGRK
                     AKAR"
     gene            complement(4114..5217)
                     /locus_tag="VKU03_13055"
     CDS             complement(4114..5217)
                     /locus_tag="VKU03_13055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889805.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HLJ72242.1"
                     /translation="MADDSAIMKALAGVAGPDGKTPLPQSGAIGGLTQRDGKVYLSIA
                     VEPARAAELEPMRLAAEKALKATPGVANALVTLTAERPPGAPVQPGAKTAHGPRPRIP
                     ESVAGVKNIIAIASGKGGVGKSTTAANLAVAMAREGWRVGLLDADVFGPSAPRLFGLK
                     EKPKVEQSFIVPLEAYGVKVISIGLLIEEEAPVVWRGPMVMSAITQLLREVAWGELDC
                     LVVDMPPGTGDVQLTMAQSAPLAGAIIVSTPQDLALIDARRGVAMFDKVKIPVLGIIE
                     NMSYFICPHCGTRSDIFAHGGARHEAEKRNVPFLGEVPLDMAIRETSDGGKPIVAAEP
                     DGAHAQSYRAIARAVIARLEGKPQRAAPRIVIG"
     gene            5343..6425
                     /locus_tag="VKU03_13060"
     CDS             5343..6425
                     /locus_tag="VKU03_13060"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72243.1"
                     /translation="MTSKRGESDSAAAPDTPERDFAPEAIAPLANLPLFFRLAGKKAV
                     IAGGSDAAAWKAELLAAAGAKVEIFAANPSRKMIEFAAGRADARIERRNWRPEDLDDA
                     AIAVGDFADPAESRAFNAAGRAAGAPVNVIDQPEFCDFSFGAIVNRSPLVIGVSTDGA
                     APVFGQALRGRLEALIPQGFAAWAAAAESWRDQAQALGASFRRRRRFWERFAALALAK
                     ADRAPSEEDRLALIAQAAGEEVSAEAGEAIFICVGQGGADLLTLRAWRALQSADAIVV
                     DDIVPPESLDLARREAQRIRARAPAENAETIVALCREGKKVVRLRGGVADISAEGDDE
                     VAALRRARIPCSIIPGARDPASERRL"
     gene            6422..7285
                     /locus_tag="VKU03_13065"
     CDS             6422..7285
                     /locus_tag="VKU03_13065"
                     /EC_number="1.1.1.290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007613905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72244.1"
                     /translation="MKLFVFGLGYSARHFVAGLRGARVVGTSREARKGTADAGVEILA
                     FDGAKADPAIEARLAESDTLLVSIPPSADGDPVLARFAGALAASPWRVIVYLSTVGVY
                     GDHGGAWVNETSALRPSSARNRARVEVEQAWLKLGRAIGAPVHILRLAGIYGPGRNAL
                     ANLREGRARRIVKPGQVFNRIHAADIAQAIGLAIERGGEGGVWNVADDEPAAPQDVIA
                     FAAGLLGMKPPPEAAFEAAEMTPMARSFYGDNKRVSNAKLKRELGFAPLYPSYREGLR
                     ALAASGEGALA"
     gene            complement(7356..7913)
                     /locus_tag="VKU03_13070"
     CDS             complement(7356..7913)
                     /locus_tag="VKU03_13070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012455262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HLJ72245.1"
                     /translation="MFIQTEATPNPATLKFLPGQPVMPEGTFEARDAAEAARSPLARA
                     LMQVPGVSGVYFAPDFISVTKSAGEWQHLRPAILGVVTEFYLSGEPILEGEAEAAEAS
                     SEFFDEKDAETVETIKELIETRVRPAVAGDGGDITFKGYRDGVVYLNMKGACSGCPSS
                     TATLKHGIQNLFHHFLPEIRAIEQV"
     gene            8198..11161
                     /locus_tag="VKU03_13075"
     CDS             8198..11161
                     /locus_tag="VKU03_13075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015823932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008115 - sarcosine oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit alpha family protein"
                     /protein_id="HLJ72246.1"
                     /translation="MTQPFRTAAGGRVDRAKPLEFAFDGVRYVGFAGDTLASALLANG
                     VHLVGRSFKYHRPRGILAAGAEEPNALVGIGASDSRFTPNLRATQVELYRGLQAVSQN
                     NWPSLRRDLQAVNGWLAPLFPAGFYYKTFMRPAAAWKALYEPLIRRAAGLGRAPKARD
                     ADRYANLYAHCDVAIVGAGPAGLAAALTASRGGGRVMVFDDQAEMGGSLLAETKARID
                     GRSAAEWLAATLAELRARPNVALVPRTQIFGYYAQNFLGGLERLSDHLAEPDPRLPRE
                     RLWQVRAKRVVLATGAHERPLVFPDNDRPGIMLADAARTYLQRYGAKPGSRALIAAGC
                     DSAYRAALELAEAGIAIALIADLRADADGHWPEAARKTGLRVETGARLRGTRGKFRVA
                     EARIETRRGAETVACDLVAMSGGWTPSLHLFSQSRGRPVFDEALQAFLPGASAQDECS
                     AGACRGVFSLAGVLKDGADAGAAAIGAAAPRFAVEGAPEAAGGVLGKPPEARYHAKAF
                     VDFQNDVTARDLALATREGFRSIEHVKRYTTSGMATDQGKTSNMNALAIAAEALGKPI
                     PEVGLTTFRLPYTPVTFAAFAHMSRRDLFDPVRKTALHDWAEEHGATFEEAGLWKRAS
                     YFMLAGETLRETHIRECLGTRQRAGMMDASTLGKLEVTGPDSVEFLNRVYVNSFDKLA
                     IGRCRYGLMLTETGFVMDDGVVARLSTDRFHVTTTSVGVARVIAQMEDFLQTEWPDLN
                     VWFTPVTEQWATIAVNGPCTRQMLAPLIEGIDISPAAMPHMSIREGRICGAPTRLARV
                     SFTGELGFEVNVPADYGRAVWEAIWAEGRRVDCVVYGLDTLLILRAEKGYIVVGQETD
                     GTVTPDDLGLGKMVAKNKPDFIGKRSLSMADLTRPGRKQLVGLMPEDTEIKLDEGAQI
                     VTEEKPAIGTSAIGHVTSAYWSPTLQRGFAMALVESGRSRMGARVHVTTTEGTLPAQI
                     VEPVFYDKEGKRLEA"
     gene            11151..11714
                     /locus_tag="VKU03_13080"
     CDS             11151..11714
                     /locus_tag="VKU03_13080"
                     /inference="COORDINATES: protein motif:HMM:NF016180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sarcosine oxidase subunit gamma family protein"
                     /protein_id="HLJ72247.1"
                     /translation="MRPDALRRPPLASLVAPRSARAEISFCRPATRLILRGREEAARA
                     AAVFGPAPPLQPLRAAEKGARAAIWQSPDEWLLIAEDAAPAPLIETLGAALAGIPHGL
                     VDVSHRQAALEIAGPGAARLLNAGVPLDLDLSAFPVGMATRTVLVKADILLWRRSAER
                     FRLEFARSFSTYVVDVLAQAAADQEMC"
     gene            11900..>13261
                     /locus_tag="VKU03_13085"
     CDS             11900..>13261
                     /locus_tag="VKU03_13085"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019906228.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HLJ72248.1"
                     /translation="MISVFELFKIGIGPSSSHTVGPMKAAAAFVEGLEARGALRAAAR
                     VVVDLYGSLAFTGKGHAVDKAVILGLSGEKPATIDPDAADRIVVETRATKSLRLAGKQ
                     GVAFDPETAIRFDTAAQPKRHPNTLRFSAFDEAGTLLLEETWCSIGGGFILREDEAEA
                     QDAANVAYPYPFRSAADLMARGRESGLSFAAMVRANEAALRPPAEIDAHVDLVISAML
                     ACIDRGLAVEGLLPGGLKVRRRAKAIYERLKANASLNARAAHEIMDFVSVYAMAVNEE
                     NAAGGKVVTAPTNGAAGVIPSVLRYYRDHCIGADREGMRAFILTATAIGALFKMNASI
                     SGAEVGCQGEVGVACSMAAAGLCAALGGTNAQIENAAEIGMEHHLGMTCDPVGGLVQI
                     PCIERNAMGAVKAINAARMALRGNGKHYVSLDKVIKTMMQTGADMKTKYKETSRGGLA
                     VNVI"
BASE COUNT         2085 a         4384 c         4512 g         2280 t
ORIGIN      
        1 gccggcgaga tgggcgacgg acgtgtcgca actaatcaca aggtcaagcg ccatcatcgc
       61 cgccgcggta tcgacaaagg cgtcgggccc ggaatcgaaa tcctcgccca gcgtctcgac
      121 tgtcatggtc ggcggcagcg ctgcgatctg ttcggtcccg aagccttttt gcagcgagat
      181 gaggcgaata tcgggcagag acgcgagcgg cgcaaacgac gacagcggca tggcccgcgc
      241 catatcggct tccggattgg cgttgccctg ccagacaatg ccgatcttga atcccgcttc
      301 gccgattcgc gccgcccatt tcctttcaag cgccggctcg gcgtggagat agggaatcgg
      361 cgtcgggatc gtgtcgagcc gcgtgccgaa ggcgcgcggc aggctgctga tcgcgatctg
      421 cgcatcatag ggcgcttcat ccatggcccc gatgaagcgc acgccgggga aggaggagag
      481 cagccgatgc agcgaaggcg ggcagaacag cgtcgtctcg acgccggcct cggccatcag
      541 cgcgacatag cggaagaact ggatcacgtc gccgagcccg tgatccttca tcaccaggac
      601 tttcgcgccc ttggtcagcg cgcccgtcca gaccggaaag cgagcgccca gcgcctcttt
      661 cagcgatttg ccgttcagcc agcgcgcctc gtagccttcc cagccgcgct cgaaatcgcc
      721 gagcgtcaga tagagacagc ccttgccgcc gatcgcgccg ttgctgccga gccgctcggc
      781 gccctcgaaa gcggccagcg cctcgtccag ccggtcgagg gcgcgcaagg ccaaggcctc
      841 gccgcaccag gccgccgcga aatcaggctt cagcgccttg gccttgcggt aaagcgcaag
      901 cgcctcctcg gcgcggtcgc gcttgagcag cgcattgccg agattgagcg ccgcctcggc
      961 gaagctcggc cgaagcttca gcgccttgcg ggccgcctcg gccgattcct ccatgcggcc
     1021 gagctcgcgc agcgcgccgg cataggcgtt ccaaccgtcg gcgtcctgcg gcgccagcgc
     1081 gacgacccgg gcgaaagccg aagcggcctc ctcatagcgg ccgagatcga acagcagctt
     1141 gccggaattc gaccaggcga tcgtgaagtc ggtcttgagc gccaaggcgc gggcgtaaaa
     1201 gcgcaaggcc gcatcggtct tgccttgctg cccgaggatg aaaccgccca tgcacagcgc
     1261 ttcgggataa tcggggttct cgcgcaaagc ggcgtccaga tgcgcaatcg cctcctgctg
     1321 gcggccggcg ttccacaacg ccgcgccatg gcgatagaaa gcctcggcgg atcgttcctg
     1381 aagggtcaaa gcgcgttccc aggcgcgaag cgccgcatgc gccgcgctca gtcgggccaa
     1441 agaacgatgc gagccttgcg cgagactggc gcttcaaaag cgtagccgcg gtcgccggcc
     1501 gcggctacga ggcgcgacgc tagccgccga gcctgaattt ctgcaggctc tgcaaaaagc
     1561 tgttgctgaa caggggcgtc ggggaatcgc tcgaactatt gagcgcggcg ctgatcgcct
     1621 ggccgttcga ggtgatcacg ccggagagga tggaggaggc ggcggagccg gcgctcgcgc
     1681 tcgcattgtt gccgccgctc acggtcagcg acgacgttga acccgaggtt gggccatatt
     1741 cgacatcgta catcgccgtg aagcgctcga ccaattgctt gagcttggtc ggattttgca
     1801 gatcggaaag cggcatgagt ttgatgattt gcttcgcttc cgcatcgata ttgttggcgt
     1861 tgctggcggg cgggagtcca aaaatcgtct ggaccacttc caagaggttc ttgtcggcca
     1921 gaattccgta ggcgctggtg accgacggcg cgacgcgctg gaaatagagc gcgagctgga
     1981 cgccgggatc ctcttcgccc tggtcgcttt ccagttgctg ctcgacgtaa ttgttcgtcg
     2041 tcgtctgaat cgccgtcttg ctcgagaccg aggcggcgcc ttggccgacg aagtcgaagg
     2101 ccgcggcgaa ggccttccac ttgccgccct gcagcgtgtt ggcgagactt ttcggattgg
     2161 agacgccgcc ttccaggatt ttggtgatca gcgccttgga attgatctga tcgcccagcc
     2221 cataggcgtc gagcgcgtag gataacaatc tgtaattgct gacgaaatca gagatcgacg
     2281 tcaccttgcc gatattcgcg gcgtaatagg cgctggccgt cttcaccgcc ggctctttgg
     2341 cggtcatgct ctgatagcgg gagaggttgt tctcgacctg gagataggcc gcggtcgtcg
     2401 tggtcacggg tgatgccggt cgagtagccc tcgcgcccgc gatttgagaa tcggacgggc
     2461 gcccgcccta gcattacggc gagatcgtta tcggtttctt aagacttgcc cgccggcccg
     2521 ctttcgccca tggggagacc gtccgctgaa ccgccgcctc accaaagcaa ccgcccgatg
     2581 ccgtcttttc tccgcctgcc gagccggctg caggtcattc tctggttcac cgcgagcgcc
     2641 gctgtggtcg gagcgctcta cgcccatttc catgccgtca tggagggcga tcggctcctt
     2701 tcgctcagcg gcgtcccgcg gggcgccatg accggcgcga tcatcgcgag cattctgatc
     2761 tctttcgaaa tctccgttgt cgccgagccg ctcggagcgc ggctgcgcca agcgcggttt
     2821 ctcctccacg tggcggtcaa gactttcttc tatctcatcg tcatcctggt cgcgcttgag
     2881 cttggcgcgt gggcgctgcc ggcggcgggc gagcgcggcg tcgagcttcg cgacgtggcg
     2941 ttctcgctcg cggcctcctt cgtcttcgtc ttcctcttcg acatcaatcg cctgctcggg
     3001 cagaacgtcc tgctcaattt cgtcaccggc cgctatcacc agccgcggct ggaggagcgg
     3061 gtgttccttt tcatcgacat ggaaggatcg acgggcttcg ccgagcggct cgggcctgtc
     3121 gcctttcatc gcctgctcaa tcggttcgtc gtcgacctca ccggcccgat cgtcgcggcg
     3181 cgcggggaag tctatcgcta tgtcggcgac gagctgatcg cgacctggag gctcaacgag
     3241 gggaccgccg acgcccgctg cgtggcggcc tgtttcgccg cgatggatcg gttcgcgctg
     3301 ctcgcctcag cctatcggcg cgagttcggc gcagcggtga attttcgcgc cggtttgcat
     3361 tgcggcccgg tggtgaccgg cgaaatgggc tcggtgaaga aggagatcgt ctttctcggc
     3421 gatacgatga acacggccgc ccgcatccag gaattctgcc gtcagaccgg cgatcgcgtg
     3481 ctggcgtcgg cggacttgct cgaccggctc gctctgccgc cgggcgtcgt caagcggccg
     3541 ctcggcgatc tccgcctgcg cggcaaggaa agccaggtgg cgctctacgc attgacgaag
     3601 acggcgcccg ggcgccggca ggctcgcgag ggaagcgtcg ccgaagcctg accggttcgc
     3661 tatcgcgcct tcgccttccg gccgagctcg gcgagatagg tccaactgaa gatgccggtc
     3721 gaatggccgt cgtcgaaagt cggccggatg gcgtaattgc cgatcggctc gatcgcggtg
     3781 atccgcgccg gccggctgcc gatcagcctt tcgcgctccg cctcggtgag tcgcttggcc
     3841 tcgacgctcg gcgtcatctt gcgcaggtac gagctcgaca gggtgaagct ttcgcccgaa
     3901 tcgagcgcga tggtcagctt gcgaccctcg tctgtcagcc gtatttcggt cggccacgcc
     3961 tcgtcagcat tctctgtcat gattgcaccg cggcgtatgc aggttggcgg gtccggccga
     4021 cggatgccgc ggcgtatccg gcgcgaagcg ccggatgagg gcgaaccgcc gcgacggtca
     4081 ttgagcctca gggccgttcg acatcaacgc gactcacccg atcacgatgc ggggcgccgc
     4141 tctctgcggc ttgccttcaa gccgcgcgat cacggcgcgc gcgatcgcgc gatagctctg
     4201 agcgtgcgcg ccgtccggct cggcggcgac gatcggtttg ccgccgtccg aggtttcgcg
     4261 gatcgccatg tcgagcggca cttcgccgag gaacggcacg ttgcgcttct ccgcctcgtg
     4321 gcgcgcgccg ccatgcgcga aaatatcgct gcgcgtcccg caatgcgggc agatgaaata
     4381 gctcatattc tcgatgatgc cgaggaccgg gattttgacc ttgtcgaaca tcgcgacgcc
     4441 gcgccgcgcg tcgatcaaag ccaaatcctg cggcgtcgag acgatgatcg cgccggcgag
     4501 cggcgcgctc tgcgccatgg tcaattgcac gtcgccagtg ccgggcggca tgtcgacgac
     4561 gaggcaatcg agctcgcccc aggcgacttc gcgcagcaat tgggtgatcg ccgacatgac
     4621 catcggcccg cgccagacga ccggcgcctc ttcctcgatc agcaggccga tcgagatcac
     4681 cttcacgcca taggcttcga gcggcacgat gaaggattgc tcgaccttgg gcttctcctt
     4741 gaggccgaaa aggcgcggcg ccgaggggcc gaacacgtcg gcgtcgagca ggccgacccg
     4801 ccagccttcc ctggccatgg cgacggcgag attggccgcg gtcgtcgatt tgccgacgcc
     4861 gcccttgccg gaggcgatgg cgatgatgtt cttcaccccg gcgacgcttt ccgggatgcg
     4921 cggccttggg ccatgcgccg tcttggcgcc gggctggacc ggcgcgccgg gcgggcgttc
     4981 ggccgtcagc gtcaccagcg cgttggcgac gcccggcgtc gccttgagcg ctttctcggc
     5041 ggcgagccgc atcggctcga gctcggccgc gcgggccggt tcgacggcga tcgacagata
     5101 gactttgccg tcgcgctggg tgaggccgcc gatcgcgccg gattgcggca gcggcgtctt
     5161 gccgtccggc ccggccacgc cggccagggc tttcatgatc gcgctgtcgt ccgccaaacg
     5221 tctctccttg ggccgcttgc gcccgaaacg gagcgagggc cgatccgcgt gtgatataga
     5281 cgggttgttt gggcgtttga aggcgcgatc gaagaagcgc tcgaatttgc aaggcgtaag
     5341 caatgacgag caagcgtgga gagtcggatt cagccgcggc gccagacacg cccgagcgcg
     5401 atttcgcgcc ggaggcgatc gcgccgctcg ccaatctgcc gctcttcttc aggctcgccg
     5461 gaaagaaggc ggtgatcgcc ggcggttcgg acgcggcggc ttggaaggcg gaactgctcg
     5521 ccgccgcagg ggccaaagtc gagatttttg ctgcgaaccc ttcccgaaaa atgatcgagt
     5581 tcgcggccgg gcgagccgat gcgcggatcg agcggcgaaa ctggcgcccc gaagacctgg
     5641 acgacgcggc gatcgcggtc ggcgatttcg ccgatccggc ggaatcgcgc gccttcaacg
     5701 ccgccggacg cgccgcgggc gcgccggtca atgtgatcga ccagcccgaa ttctgcgatt
     5761 tctccttcgg cgcgatcgtc aaccgctcgc cgctcgtcat cggcgtctcg accgacggcg
     5821 ccgccccggt tttcggccag gctttgcgcg gccggcttga agcgctcatc ccgcaaggct
     5881 tcgccgcctg ggccgcggcc gccgagagtt ggcgggatca ggctcaggcg ctcggcgcga
     5941 gctttcgccg ccgccgccgc ttttgggagc gcttcgccgc tttggctctg gccaaggccg
     6001 accgcgcgcc gagcgaagag gatcgcctgg cgctgatcgc acaagcggca ggcgaggagg
     6061 tttcagccga agcaggcgaa gcgatcttca tttgcgtcgg gcagggcggc gccgatttgc
     6121 tgacgttgag ggcgtggcgc gctttgcaat ccgcggacgc catcgtcgtc gacgacatcg
     6181 ttccgccgga gagcctcgat ctggcgcgcc gcgaagcgca gcggatcaga gccagggcgc
     6241 cggccgagaa tgccgaaacc atcgttgcgc tctgccgcga aggcaagaag gtggtgcggc
     6301 ttaggggcgg cgtcgcggat atcagcgcgg aaggcgacga cgaggtcgcc gctttgcgcc
     6361 gagcgagaat cccgtgctcg atcattcccg gcgcccgcga cccagccagc gagcgcaggc
     6421 tgtgaagctc ttcgtgtttg gcctcggcta cagcgcccgc cattttgtcg ccggcttgcg
     6481 cggcgcgcgg gttgtcggca cgtcgcgcga ggcgcgcaaa ggaacggcgg atgccggcgt
     6541 cgaaatcctc gctttcgatg gcgcgaaagc tgaccccgcg atcgaggcgc gcctggccga
     6601 aagcgacacg ttgctcgttt cgatcccgcc gtcggcggac ggcgatccgg ttctcgcgcg
     6661 ttttgccggc gcgctcgccg cctcgccttg gcgggtcatt gtctatttgt cgacggtcgg
     6721 cgtttacggc gaccacggcg gcgcgtgggt caacgagacg agcgccttgc ggccaagctc
     6781 ggcgcgcaac cgggcgcggg tcgaggtcga gcaggcctgg ctcaagcttg ggcgcgccat
     6841 cggcgcgccg gtccacatcc tgcgcctggc cggcatttac gggcccggcc gcaacgcgct
     6901 ggctaatctg cgcgaaggcc gggcgcggcg catcgtcaag cccggccagg tcttcaaccg
     6961 cattcatgcc gccgacatcg cccaggcgat cggccttgcg atcgaaaggg gcggcgaggg
     7021 gggcgtttgg aatgtcgccg acgacgagcc ggcggcgccg caagacgtga tcgccttcgc
     7081 cgccggcctg ctcggcatga agccgccgcc cgaagcggcg tttgaagccg ccgagatgac
     7141 gccgatggcg cgcagcttct atggcgacaa caagcgggtc tccaacgcca agctgaaaag
     7201 ggagcttggc tttgcgccgc tttatccgag ttaccgggaa ggacttcgcg ctctcgccgc
     7261 gtcgggcgaa ggcgcgctcg cctgaagccg catctttttt tggcgggccg gcaaacggaa
     7321 aaaccgcttg cgcggctcac tgccgccttt agaggtcaga cttgctcaat ggcgcggatt
     7381 tcgggcagga aatggtggaa caggttttga atgccgtgct tgagcgtcgc ggtcgaggag
     7441 gggcagccgg agcaggcgcc cttcatgttg agatagacga cgccgtcgcg atagcccttg
     7501 aaggtgatgt cgccgccgtc gccggccacc gccggccgga cccgggtctc gatcaattcc
     7561 ttgatcgtct caaccgtctc ggcgtctttc tcatcgaaga attcgctgga agcttcggcc
     7621 gcttcggcct cgccttcgag gatcggctcg ccggacaagt agaattccgt caccacgccg
     7681 agaatggccg gccgcaaatg ctgccattcg ccggcgcttt tggtgaccga gatgaaatcc
     7741 ggcgcgaaat agacgccgct cacccccggc acttgcatca aggcccgagc gagcggcgag
     7801 cgcgccgcct cggccgcgtc gcgcgcttcg aacgtgccct ccggcatgac aggctgtccc
     7861 ggcaggaatt tcaacgtcgc cgggttcggc gtggcttcgg tctggatgaa caaggggcgg
     7921 actccgcgaa aaggggcaag ctttggagtg aaaataggaa ccgaggccgg cggatgcaag
     7981 ccagaggcgc cggcgcatcc cttttccccg caacgcgggg agaacgtggc ccgaagggcc
     8041 ggatgagggc cggcgctgag gttggggatt ggcctttcgc ggacagccga ggaggcgcca
     8101 accggcaagc gcggcgctgc ccctcatccg gcggcttcgc cgccaccttc tccccgcgtt
     8161 gcggggagaa gggagcgtct ggcggggagg cggggcgatg acccagccct tccgcacggc
     8221 cgccggcggc cgggtcgacc gcgccaagcc gctcgaattc gccttcgacg gcgtccgcta
     8281 tgtcgggttc gccggcgata cgctggcttc ggcgctcctc gccaacggcg tccatctcgt
     8341 cggtcgctcg ttcaaatatc accgcccgcg cggcatattg gcggcgggcg cggaggagcc
     8401 taacgcgctg gtggggatcg gcgccagcga cagtcgcttc acgccgaatc tgcgcgcgac
     8461 gcaagtcgag ctttaccgcg gcttgcaagc cgtcagccag aacaattggc cctcgctccg
     8521 ccgcgacttg caggcggtca atggctggct tgcgccgctc tttccggccg gcttctacta
     8581 taagaccttc atgcggcctg ccgcggcctg gaaggccctc tatgagccgc tgatccggcg
     8641 cgccgccggc ctcggccggg cgccgaaagc gcgcgacgcc gaccgctacg ccaatctcta
     8701 cgcccattgc gacgtcgcga tcgtcggcgc cgggccggcc ggtctcgccg ccgccttgac
     8761 ggcgtcccgc ggcggcggcc gcgtcatggt cttcgacgat caggcggaaa tgggcggctc
     8821 gctgctcgcc gaaaccaagg cgcggatcga tggccgaagc gccgccgaat ggctcgccgc
     8881 gacgctcgcc gaattgcgcg ccagacccaa tgtcgcgctc gtcccgcgca cgcagatttt
     8941 cggctattac gcgcagaatt tcctcggcgg cctggagcgg ctcagcgatc atctcgccga
     9001 gcctgatccg cgcctgccgc gcgagcgcct atggcaggtc cgcgccaagc gcgtcgtgct
     9061 ggcgaccggc gcgcatgagc ggccgcttgt ctttcccgac aacgaccggc cggggatcat
     9121 gctcgccgat gcggcgcgga cttatctcca gcgctacggc gccaagcccg gttcgcgcgc
     9181 gctgatcgcc gcggggtgcg attcggccta ccgcgccgcc ttggaattgg ccgaggctgg
     9241 aatcgcgatc gcgctgatcg ccgatttgcg cgccgatgcg gatggccatt ggccggaagc
     9301 ggcgcgcaaa acgggcttgc gcgttgagac cggcgcccgc cttcgcggta cgcgcggcaa
     9361 atttcgcgtc gccgaagcgc ggatcgaaac gcgccgcggc gccgagacgg tcgcctgcga
     9421 tcttgtcgcg atgagcggcg gctggacgcc gagccttcat cttttctcgc aatcgcgcgg
     9481 tcggccggtt ttcgacgagg ctttgcaggc cttcctcccc ggcgcctcgg cgcaggacga
     9541 atgctccgcc ggcgcctgcc gcggcgtctt ttctctggct ggcgtcctca aggacggcgc
     9601 cgatgcgggc gccgccgcga tcggcgcggc cgcgccgcgc ttcgccgtcg aaggcgcgcc
     9661 ggaagcggcc ggcggggtgc tcggcaagcc gccggaggcc cgctatcacg ccaaggcttt
     9721 tgtcgatttc cagaacgacg tcaccgcgcg cgatcttgcg ctggcgacgc gcgaaggctt
     9781 ccgttcgatc gagcacgtca agcgctacac gacgagcggc atggcgaccg accagggcaa
     9841 gacctcgaac atgaacgcgc tcgccatcgc cgccgaggcg ctcggcaagc cgattccgga
     9901 agtcggcctg accacgttcc gcctgcctta cacgccagtc accttcgccg cgttcgccca
     9961 tatgtcgcgg cgcgacctgt tcgatccggt gcgcaagacg gcgctgcatg attgggcgga
    10021 ggagcacggc gcgactttcg aggaagcggg cttatggaag cgagcgtcgt actttatgct
    10081 tgccggcgag acgttgcgcg agacccatat ccgcgaatgt ctcggcacgc gccagcgcgc
    10141 cggcatgatg gacgcctcca ccctcggcaa gctcgaagtg accgggcccg attctgtcga
    10201 gttcctcaac cgcgtttacg tcaattcctt cgacaagctc gcgatcgggc gctgccgcta
    10261 cggcctgatg ctgaccgaga ccggcttcgt catggacgac ggggtcgtcg cccgcctgtc
    10321 gacagaccgc ttccacgtca cgacgaccag cgtcggcgtc gcgcgagtga tcgcccagat
    10381 ggaggatttc ctgcagaccg aatggccgga ccttaatgtc tggttcacgc cggtcaccga
    10441 gcaatgggcg acgatcgcgg tcaatgggcc atgcacgcgc cagatgcttg cgccgctgat
    10501 cgaagggatc gacatttcgc ccgccgccat gccgcatatg agcatccgcg agggccgcat
    10561 ctgcggcgcg ccgacccgtc tcgcccgcgt cagcttcacc ggcgagctcg gcttcgaggt
    10621 caatgtgccg gccgactacg gccgcgccgt ctgggaggcg atctgggccg agggacgaag
    10681 ggtcgattgc gtcgtctatg gcctcgatac gctgctgatt ttgcgcgccg agaagggcta
    10741 tatcgtcgtc ggccaggaga ccgacggaac ggtgacgccg gacgatctcg gcttgggcaa
    10801 gatggtcgcc aagaacaagc cggatttcat cggcaagcgc tcgctgtcta tggcggactt
    10861 gacgcgaccg ggccgcaagc agctcgtcgg gttgatgccg gaagataccg agatcaagct
    10921 cgacgaaggc gcgcagatcg tgaccgagga gaagccggcg attggtacga gcgcgatcgg
    10981 ccatgtcact tcggcctatt ggagtccgac tttgcagcgc ggtttcgcca tggcgttggt
    11041 cgaaagcggg cgcagccgga tgggcgcgcg cgtccatgtg acgacgacgg agggaacgct
    11101 gccggcgcaa atcgtcgagc cggtcttcta cgacaaggag ggcaagcgcc ttgaggcctg
    11161 acgccttgcg ccgcccgccg ctggcgagcc tcgtcgcgcc gcgttcggcg cgcgcggaaa
    11221 tctccttctg caggccggcg acgcggctga tcctgcgcgg ccgcgaggag gcggcgcggg
    11281 cggcggcggt cttcggtccg gcgccgccgc tgcaaccgct cagagccgcc gagaaaggcg
    11341 cccgggcggc gatctggcaa agccccgacg aatggctgct gatcgccgaa gacgccgcgc
    11401 ccgcgccgct gatcgagacg cttggcgcgg cgctcgccgg gataccgcat ggcctcgtcg
    11461 atgtctcgca tcgacaagcg gcgcttgaga tcgccgggcc gggcgcggcg agattgctca
    11521 acgccggcgt gccgcttgat ctcgatctct cggcctttcc tgtcggcatg gcgacgcgca
    11581 ccgtgctcgt caaggccgac atcctgctgt ggcggcgcag cgcggaacgg ttcaggctcg
    11641 aattcgcccg ctctttttcg acatatgtcg tcgatgtgct cgcgcaagcc gcggccgatc
    11701 aggaaatgtg ctaaggcgcg catcccctcg ccccgcgcag cggggagagg gtggccccga
    11761 agggccgggt gaggggcagc gccggccttt gggattagcg ttcctaggtt cgtaccttga
    11821 aagacctgcg ccgcccctca tccggccgct tcgcgaccac cttctccccg ctttgcgggg
    11881 aggagggtag gagactccga tgatcagcgt cttcgaactc ttcaagatcg gcatcggccc
    11941 gtcctcatcg cataccgtcg ggccgatgaa ggcggcggcg gctttcgtcg aaggccttga
    12001 agcgcgcggc gcgctccgcg ccgcggcgcg cgtcgtcgtc gacctctatg gctcgctcgc
    12061 ttttaccggc aagggccacg ccgtcgataa ggcggtgatc ctcgggcttt ccggcgaaaa
    12121 gccggcgacg atcgacccgg acgcggcgga tcggatcgtc gtcgagacgc gggcgacgaa
    12181 gagcctgcgt ctcgccggca agcaaggcgt cgccttcgat cccgagacgg cgatccgctt
    12241 cgacacggcg gcgcagccga aacgtcatcc caatacgttg cggttcagcg ccttcgacga
    12301 ggcggggacg ctgttgcttg aagagacctg gtgctcgatc ggcggcggct tcatcctgcg
    12361 cgaggacgaa gccgaggcgc aggacgcggc gaatgtcgcg tatccctatc cgttccgctc
    12421 cgccgccgac ctgatggcgc gcggaaggga gagcggccta tccttcgccg cgatggtgcg
    12481 cgccaacgag gcggcgctgc ggccgcccgc cgaaatcgac gcgcatgtcg atctcgtgat
    12541 ttcggcgatg ctcgcctgca tcgatcgcgg gctcgctgtc gagggtcttc tgcccggcgg
    12601 gttgaaagtg aggcgccgcg ccaaggcgat ctacgaaagg ctgaaagcca acgcttcgct
    12661 caacgcccgc gccgcgcacg agatcatgga cttcgtctcg gtctacgcca tggcggtgaa
    12721 cgaggagaac gcggcgggcg gcaaggtcgt caccgcgccg accaatggcg cggccggcgt
    12781 cattccttcc gtgctgcgct actatcgcga tcattgcatc ggcgcggatc gggagggcat
    12841 gcgcgctttc atcctgaccg cgaccgcgat cggcgcgctg ttcaagatga acgcctcgat
    12901 ttccggcgcc gaggtcggct gccaaggcga agtcggcgtc gcctgctcga tggcggcggc
    12961 gggcctatgc gccgcgctcg gcgggaccaa tgcgcagatc gagaacgccg ccgagatcgg
    13021 catggagcat catctcggca tgacctgcga tccggtcggc ggcctggtgc agattccgtg
    13081 tatcgagcgt aacgcgatgg gcgcggtcaa ggccatcaac gccgcgcgga tggcgctgcg
    13141 cggcaacggc aagcactatg tgtcgctgga caaggtcatc aaaaccatga tgcaaaccgg
    13201 cgccgatatg aagaccaagt acaaggaaac gtcgcgtggc ggtttggcag tcaacgtgat
    13261 c
//
LOCUS       DATGJW010000438         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1533436, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000438 DATGJW010000000
VERSION     DATGJW010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1533436"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..870
                     /locus_tag="VKU03_13090"
     CDS             <1..870
                     /locus_tag="VKU03_13090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013529692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72249.1"
                     /translation="WGLGSSLAMESIPPRARGAVSGFLQCGYPTGYLVAAIAYGLLYQ
                     RNFGGFVVGWRAMFLLSIVPALVVLFIRSHVPESPAFVEAQKGPRPSLMETLRAHWGL
                     ALYSVVLMMCFNLFSHGTQDLYPTFLQTQLKLDQATVSWITIVANIGAIVGGLFFGYI
                     SERIGRVNAITIATLIALPALPLWVYTTTPFLLALGAFVMQISVQGAWGIIPAHLNEL
                     SPGPVRATLPAFVYQAGNFLASYNGPFQAKIAQGSSYSFALALIAGIVAVAIIIVIRF
                     SPERRGEVLSVAH"
     gene            complement(967..1956)
                     /locus_tag="VKU03_13095"
     CDS             complement(967..1956)
                     /locus_tag="VKU03_13095"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009492249.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HLJ72250.1"
                     /translation="MTALSILDLAYIAQGSSLGDALRHSLDLARHAEGWGYKRFWLAE
                     HHNMPGIASAATAVAIGYVAGGTKTIRVGSGGIMLPNHAPIVIAEQFGTLEALYPGRI
                     DLGLGRAPGTDQRTVRALRRDPAAADNFPQDVMELQAFLAPAQPNQPVRAVPGAGSNV
                     PLWILGSSLFGAELAAMLGLPYAFASHFSPEALLDALELYRARFEPSEQLSQPYVMAG
                     AAAIVADTDEEARYLFTTPQQQVTNMLRGVRGQLQPPIDDIETYWNPAEKAQASRMLT
                     YAFVGSRETVRDKLRRFIDLTKADEVMVASAIYDHGARLRSYEILAEIGRELA"
     gene            complement(2242..3183)
                     /gene="miaA"
                     /locus_tag="VKU03_13100"
     CDS             complement(2242..3183)
                     /gene="miaA"
                     /locus_tag="VKU03_13100"
                     /EC_number="2.5.1.75"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335835.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0052381 - tRNA dimethylallyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (adenosine(37)-N6)-dimethylallyltransferase
                     MiaA"
                     /protein_id="HLJ72251.1"
                     /translation="MGQGGMKRRALLIAGPTASGKSAVALALARRFGATIVNADSMQV
                     YRDLRLLTARPSEADEALAPHVLFGVVDGAVNHSVGLWLAEAREAAAHAFAEGRPVLY
                     VGGTGLYFRALTEGLSNIPEVPATIRARIRAEAEGRPTPDLHAKLAKTDSETASRLRP
                     SDRQRVLRALEVFAAAGRPLAAFQGARGAPFLRSGEWAGIFLAPERAALRAAIDARFD
                     AMLAAGALDEVAALAARRLDPALPVMRAHGVPHLIAHLAGRLPLEEAAARGKLDTRQY
                     AKRQFTWARHQMTGFEWVAPDEAKRAGAGALARLTED"
     gene            3196..>3505
                     /locus_tag="VKU03_13105"
     CDS             3196..>3505
                     /locus_tag="VKU03_13105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111382.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoserine phosphatase SerB"
                     /protein_id="HLJ72252.1"
                     /translation="MPSRRFVVTLVAAPQSFDLSDALIARIAQALPGKVEVSRLAAGA
                     ADLFFMAEGESAAAKARLAAALEGEAVDAIVQPAEGRRKRLLLADMDSTLIQQECVDE
                     L"
BASE COUNT          545 a         1173 c         1186 g          601 t
ORIGIN      
        1 tggggccttg gctcctcgct cgccatggaa tcgattccgc ctagggcgcg cggcgccgtg
       61 tcaggtttcc tgcaatgcgg ctatccgacc ggctatctcg tcgccgcgat cgcctatggc
      121 ctgctttatc agcgcaattt cggcggtttc gtcgtcggct ggcgggcgat gtttctcctt
      181 tccatcgtgc cggcgctcgt cgtcctcttc atccgctcgc atgtcccgga atcgccggct
      241 ttcgtcgagg cgcaaaaagg gccgcggccg agcctgatgg agacgttgcg cgcccattgg
      301 ggcttggcgc tctattccgt cgtgctgatg atgtgcttca acctcttcag ccacggcacg
      361 caggatctct atcccacctt cctgcagacg cagctcaagc tcgatcaggc cacggtcagt
      421 tggatcacca ttgtcgccaa tatcggcgcg atcgtcggcg gcctgttctt cggctacatc
      481 tcggagcgga tcggccgcgt caacgcgatc accatcgcga cgctgatcgc tttgccggcc
      541 ttgccgcttt gggtctatac gacgacccca ttcctgctcg cgctcggcgc cttcgtcatg
      601 cagatctcgg tgcagggcgc gtggggcatc atcccggcgc acctgaacga actctcgccg
      661 ggtccggtgc gcgcgacgct gcccgctttc gtctatcagg ccggcaattt cctcgcttcg
      721 tataacgggc cttttcaggc caagatcgcc cagggcagca gctacagctt cgcgctcgcc
      781 ctgatcgccg gaatcgtcgc tgtggcgatc atcatcgtga tccgcttcag tcccgagcgg
      841 cggggggaag tcctgtcggt cgcgcattga ggatgagcgc cggcatttgc gaggagcgca
      901 gcgacgaagc gatccattca tggcggcgct ctaggagggg attgtttcgc ttcgcccgca
      961 gggcggtcag gccagttccc gcccgatctc ggcgagaatc tcgtacgagc gtagccgcgc
     1021 gccatggtcg tagatcgccg aggcgaccat cacttcgtcg gccttggtca aatcgatgaa
     1081 gcggcgaagc ttgtcgcgca cggtctcgcg cgagccgaca aaagcgtagg tcagcatgcg
     1141 cgaggcttgc gccttctcgg ccggattcca ataggtctcg atgtcgtcga tcggcggctg
     1201 caattggccg cgcacgccgc gcagcatgtt cgtgacctgc tgttgcggcg tggtgaagag
     1261 atagcgcgcc tcctcgtccg tatcggcgac gatcgcagcg gcgccggcca tgacataggg
     1321 ctgagagagc tgttcggacg gctcgaaccg ggcgcggtag agctccagag cgtccagcaa
     1381 agcctccggc gaaaaatgtg aggcgaaggc gtaaggcagg ccgagcatcg cggcgagctc
     1441 agcgccgaac aagctggagc cgagaatcca gagcggcacg ttgctgccgg cgccgggcac
     1501 ggcgcgaacc ggctggttcg gctgcgccgg cgcgagaaag gcttgcagct ccatcacgtc
     1561 ctgcgggaaa ttgtcggccg ccgccggatc gcggcgcagc gcgcggacgg tacgctgatc
     1621 ggttcccggc gcgcggccga ggccgagatc gatgcggccg ggatagagcg cctccagcgt
     1681 gccgaactgc tcggcgatga cgatcggcgc atggttgggc agcatgatcc cgcccgagcc
     1741 gactctgatc gtcttggtgc cgccggcgac atagccgatc gccaccgcgg tcgcggcgct
     1801 ggctatgccc ggcatattgt gatgctcggc cagccagaag cgcttatagc cccagccttc
     1861 ggcgtgccgg gcgagatcga gggaatggcg cagcgcgtcg ccgagcgacg agccctgcgc
     1921 gatataagcg agatcgagaa tggagagagc ggtcatgcgg aagctcgttt ggcgcggttt
     1981 gtcgctcggc gattaagccg ccatcgttga ggacggagca gttcgttatc taggcgcaac
     2041 gccagggcct cctctccccg cttgcgggga gacgtacggt gaggggcttg gggagcaaag
     2101 accgacaact gggatcaagc gacatgcccc tctccgtacc tctccccgtc ccgcttcgct
     2161 ccacggggag agggggccgg gacgccgcgc caatatgcga cgacacggcg tccgcccatg
     2221 tgggactttc gccaggaatt tctagtcctc cgtcaatcta gcgagcgccc ctgctcccgc
     2281 ccgcttcgcc tcgtccggcg cgacccattc gaaccccgtc atctggtggc gcgcccaggt
     2341 gaattgccgc ttggcgtatt ggcgcgtgtc gagcttgccg cgcgccgccg cctcctcgag
     2401 cggcaggcgg ccggcaagat gggcgatcaa atgcgggacg ccgtgcgcgc gcatgacggg
     2461 caaagccgga tcgagccggc gcgcggcgag ggcggcgact tcgtcgagcg cgccggcggc
     2521 gagcatcgca tcgaaacgag cgtcgatcgc cgctcggagc gcggcgcgct cgggcgccag
     2581 gaaaattccg gcccattcgc cggatcggag gaacggcgcg ccgcgcgcgc cctggaaggc
     2641 ggcgagcggc cggcccgccg cggcgaagac ttcgagcgcg cgcagcaccc tctgtcggtc
     2701 gctcggccgc aggcgcgaag cggtctcgga atcggtcttg gcgagtttgg cgtgcaagtc
     2761 cggcgtcggc cggccttccg cttcggctcg gatgcgcgcg cggatcgtcg cgggcacttc
     2821 cggaatattg gagagccctt cggtcaaagc gcggaaataa agaccggtac cgcccacata
     2881 aaggaccggc cggccttcgg cgaaggcatg cgccgccgcc tcccgagcct cggcgagcca
     2941 caggccgacc gaatggttga ccgcgccgtc gaccacgccg aacaaaacat gcggcgcgag
     3001 cgcctcgtcc gcctcgctcg gccgcgccgt caggaggcgc aagtcgcgat agacctgcat
     3061 ggagtcggcg ttgacgatgg tcgcgccgaa gcgccgcgcc aaggccagcg cgaccgcgga
     3121 cttgccgctc gccgtcgggc ctgcaatgag gagggcgcgg cgcttcatgc cgccttgtcc
     3181 caacccgcgg atgtcatgcc gtcccgccgt ttcgtcgtca cgctcgtcgc cgcgccgcaa
     3241 tcattcgact tgagcgatgc gctgatcgcc cgcattgcgc aagccttgcc cggcaaggtg
     3301 gaggtctcgc gccttgccgc cggcgcggcc gatctcttct tcatggccga gggcgaaagc
     3361 gccgccgcta aggcgcgcct cgccgccgcg ctcgaaggcg aggcggtcga cgcgatcgtc
     3421 cagccggccg agggccggcg caagcgcctg ctgctcgccg atatggattc gaccctgatc
     3481 cagcaggaat gcgtcgacga gctcg
//
LOCUS       DATGJW010000439         7552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_813389, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000439 DATGJW010000000
VERSION     DATGJW010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7552
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_813389"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..377
                     /locus_tag="VKU03_13110"
     CDS             <1..377
                     /locus_tag="VKU03_13110"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HLJ72253.1"
                     /translation="ALWQALHPHLGFIPGQKSGIAYGLAARLAADGESLFYLAGVEVS
                     DLSDLDDGFTGIKLPAQRWAVFPHRGHITTIAATIDAIFADALPAAGLRHGEMPDLLE
                     RYDERFDPLTGAGGFEIWVPIK"
     gene            complement(809..952)
                     /locus_tag="VKU03_13115"
     CDS             complement(809..952)
                     /locus_tag="VKU03_13115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72254.1"
                     /translation="MAAIADVLLDTNLLISPLPIWEEDWNHPETHSNPRLLENIRREG
                     LAL"
     gene            complement(1195..2715)
                     /gene="trpE"
                     /locus_tag="VKU03_13120"
     CDS             complement(1195..2715)
                     /gene="trpE"
                     /locus_tag="VKU03_13120"
                     /EC_number="4.1.3.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012116348.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004049 - anthranilate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="anthranilate synthase component I"
                     /protein_id="HLJ72255.1"
                     /translation="MIAGQEYDEIARRYEAGRASVAVSTLVADLETPVSAFLKLSAGR
                     AGNVFMLESVEGGAQRGRYSMIALDPDVIWRAHGDRCEINRRALWEREAFAPCPGKPL
                     DALRALLAESRIDMPPGLPPMAAGILGYLGYDMVRQMERLAPAKPDPIGVPEAMLIRP
                     TIVVAFDSVRDEISVVTPIRPSPGVSARAAYESAQARLEAIVATLEGPLAHTAPAVDP
                     ALMADEAQSNTTEAEFHAMVEKAKDYIVAGDIFQVVLSQRYTSRFDLPAFSLYRALRR
                     INPSPYLCYLDFGAFQVVVSSPEILVRLRGGRVAIRPIAGTRWRGKTKEEDEKLAAEL
                     LADPKERAEHLMLLDLGRNDVGRVAKIGTVEVTDSFFIERYSHVMHIVSHVEGDLDPR
                     RDAVAALAAGFPAGTVSGAPKVRAMEIIDELEKDKRGIYAGCIGYFGASGDMDTCIVL
                     RASVVKDGLMHVQAGAGIVYDSDPAYENRECANKAKALFRAAEEAVRFATGAKRGQ"
     gene            complement(2712..4610)
                     /locus_tag="VKU03_13125"
     CDS             complement(2712..4610)
                     /locus_tag="VKU03_13125"
                     /inference="COORDINATES: protein motif:HMM:NF025010.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SurA N-terminal domain-containing protein"
                     /protein_id="HLJ72256.1"
                     /translation="MLGFMRSASQGFAGRAIMAIVLGVIILSFAWWGTGNPFEGYGKN
                     DVASVGGEAISTQAFQRDYQNLLQQYQRQLKAPITNAQAHAMGLDSQVLGRMIADAAL
                     DARARSLGLAISDDTIAKAVRESPEFQDAAGRFDRQRFDEALRESGLSESGFFAEQRK
                     RSLRQEIGLSLAGNLTAPKVLVEALAHFGSESRSVDYAILPTSAAGDIPTPTPEQLQS
                     FFNDRKANYRAPEYRAINLLAVTPSTIAKPDEVSDADARALYERVKDQRFTTPEKRDV
                     QQIVFSDEAKAAAAEAKIKSGTPFDEIAKASDSGGKFVDLGETTKTGIFDKAVADAAF
                     ALPDGGVSDVVKGQFGPVIAHVTKITPSSVKPFDEVAAEMKKEVATQRAADEALSIHD
                     KIEDARASGKTLTEAAKTVGLDVRAIPAIDAEGLDKDGKAVPGIPDEKDVLRAVYASD
                     IGVDDQPVSTPDNGFVWFEVTKIDPPHDRTLDEVKDQVTKQWRDEEVAKALSKKAADM
                     VIKLDNGATLASLAEADKLDVKSAADIRRSGGAGLAENVTTAIFNAPSSGAGSATVGD
                     GRLVFKVTKDTIPPVDFSSAETKQLQQRADAALVNDMFGEYIAALQRELGVTINQSVL
                     QTAIGGIS"
     gene            4743..5507
                     /gene="tpiA"
                     /locus_tag="VKU03_13130"
     CDS             4743..5507
                     /gene="tpiA"
                     /locus_tag="VKU03_13130"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408684.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HLJ72257.1"
                     /translation="MTPGVRPLVAGNWKMNGLKASLGEIAAMRKAAEAGAAGAAELAV
                     CPPATLLSEAAALVGGGPVALGGQDCHAKASGAHTGDIAAAMLKDAGASYVIVGHSER
                     RTDHGESDALVRAKAEAALAAGLAAIVCVGETRAEREAGGAISVTSRQIKGSLPPLAD
                     PAHIVVAYEPVWAIGTGLTPTAADVAEMHAAIRSLLGEIYGPAGATIRILYGGSVKPA
                     NAAELLSLANVDGALVGGASLKAADFLGIAGVYRGA"
     gene            complement(5550..5822)
                     /locus_tag="VKU03_13135"
     CDS             complement(5550..5822)
                     /locus_tag="VKU03_13135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HLJ72258.1"
                     /translation="MCRNIRTLFNFEPPATEEEVRASALQFVRKLSGFAHPSKANQAV
                     FDQAVEDVAAAARRLLASLQTNAPTRDRASEAAKAQERARQRFAQA"
     gene            complement(6263..6997)
                     /locus_tag="VKU03_13140"
     CDS             complement(6263..6997)
                     /locus_tag="VKU03_13140"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018646377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HLJ72259.1"
                     /translation="MSELRNRVALVTGGSRGIGRAIALTLAKAGAALAVNYRERSDEA
                     AAVVEAIHKGGGRATAIGADVSSRAAVQNMVYEIEERLGPIDILINNAGMAAARSIEE
                     IVEEDFDRAIAVNLKSAFLCTQAVLPGMRARRWGRIVNISSVAARAAGAVSVAYNASK
                     AGLEGLTRGYSARLAPEGVTVNAIAPGLIETDMGRPLIEQGFAARIPVGRFGSGDEIA
                     QAVLMLVGNAYMTGQTVAVNGGVLFS"
     gene            7148..>7552
                     /locus_tag="VKU03_13145"
     CDS             7148..>7552
                     /locus_tag="VKU03_13145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008970000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HLJ72260.1"
                     /translation="MSLEIALTLIGGPTVLIEMAGLRFVTDPTFDQPGLYQGGVTLEK
                     TTAPALSAEKIGRVDAVLLSHDQHFDNLDHGGRAFLAQARTTFTTPVGAARLGGNAVG
                     LEAWRSRKFESDKGAVHVTAAPARHGPVGIEPI"
BASE COUNT         1277 a         2472 c         2479 g         1324 t
ORIGIN      
        1 cggcgctttg gcaggcgctg catccccatc tcggcttcat ccccggccag aaaagcggga
       61 tcgcctacgg cctcgccgcg cgcttggccg ccgacggcga gagcctcttc tacctcgctg
      121 gcgtcgaggt ttcggacctc tccgatctcg acgacgggtt cacagggatc aagctgccgg
      181 cgcagcgttg ggcggttttt ccgcatcgcg gccacatcac gacaatcgcc gcgaccatcg
      241 acgcgatttt cgccgacgcg ctgccggcgg cgggcttgcg gcatggcgag atgcccgatt
      301 tgctcgaacg gtacgacgaa cggttcgatc cgttgaccgg ggcagggggg ttcgagatat
      361 gggttccgat taaatagaca gcgctcggga aaatgagcgt tgttcagcgc ctcgtcgatt
      421 gaggaatatt cagttggaac cgctcggcca cggctttgat gaacgcctcg gcccattcga
      481 ccgctcggct tgcctctggg gcgccgatcg ggtctccttg gtagtcggcg agcaacctaa
      541 tgtattgcgc ctgcccaagc gtgcgcccga tttcccgttc gagcaagccc gggagcgatg
      601 atgaagcggg caaacgccgt gattagaccg ctatgagtgc gtgcgctctc ggcctcggcc
      661 ggcgcccgcg cgacaatgag ggccgcccgg gccgcatcga acattccata gtaggccctg
      721 ctcgcagctt cgtcgttgaa gccattttcg agaagcaggc gcgccgcgcc aagcgatcgc
      781 gaggctttgc gcattaaatc ttgcgctgtc acaacgcgag cccttcgcgc cgaatattct
      841 ccaacaatct gggattggaa tgggtctcgg gatgattcca gtcctcctcc cagatcggca
      901 aaggtgaaat cagaagattg gtatcgagca ggacgtcggc gatggcggcc atatcgatcc
      961 cgacttcaat cgcgctcccg cgcggtccct caaaaacacc gcgaggtcgg cgtcgctttc
     1021 gtccgagtga ctggcgcgag cgcggctgcc atagaggcgc gcgccggcaa caggaaaacg
     1081 cgttgcgatc ctctcaagga acaggcggat agcgcgttcg gtcttggggt caatggcgcc
     1141 catcagctta agatatgccg ggcaagggac gattgacagc ccctccggga gggctcactg
     1201 cccgcgcttc gcgcctgtcg cgaagcgcac cgcttcttcc gccgccctga acagcgcctt
     1261 cgccttattg gcgcattcgc gattctcgta agccggatcg gaatcgtaga cgatgccggc
     1321 gccggcctgc acatgcatca ggccgtcctt gaccaccgaa gcgcgcagca cgatgcaggt
     1381 gtccatgtcg ccggaggcgc cgaaatagcc gatgcagccg gcatagatgc cgcgcttatc
     1441 cttctccaat tcgtcgatga tctccatcgc ccgtactttc ggcgcgccgg agaccgtgcc
     1501 ggcgggaaag ccggccgcga gcgccgcgac cgcgtcacgg cgcggatcga ggtcgccctc
     1561 gacatgcgag acgatgtgca tcacgtggct gtagcgctcg atgaagaagc tgtcggtcac
     1621 ttcgaccgtg ccgatcttgg cgacgcggcc gacatcgttg cggccgagat cgagcagcat
     1681 cagatgttcg gcgcgctctt tcggatcggc gagcagctcg gcggcgagct tctcgtcctc
     1741 ctccttggtc ttgccgcgcc agcgcgtccc ggcgatcggc cggatcgcca ctcggccgcc
     1801 gcgcaaccgc accaggattt ccgggcttga gaccacgacc tgaaaggcgc cgaaatcgag
     1861 gtagcaaagg tacggcgacg gattgatccg ccgcaaggcg cgatagagcg agaaggcggg
     1921 cagatcgaac cggctcgtgt agcgctgcga cagcacgacc tggaagatgt cgccggcgac
     1981 gatatagtcc ttcgccttct cgaccatggc gtggaactcg gcctcggtcg tattcgactg
     2041 cgcttcgtcc gccatcagcg ccggatcgac cgcgggcgcg gtatgggcga gcgggccctc
     2101 caatgtggcg acgatcgcct ccagccgcgc ctgcgcgctt tcataagccg cgcgcgcgga
     2161 gacgccgggc gaaggccgga tcggcgtgac gaccgagatc tcgtcgcgca cgctgtcgaa
     2221 cgcgacgacg atggtcggcc ggatcagcat agcctcggga acgccgatcg ggtcgggctt
     2281 ggccggggcc agccgctcca tctggcggac catgtcgtag ccgagatagc ccaaaatccc
     2341 ggccgccatc ggcggcaggc cgggcggcat gtcgatgcgc gattcagcga gcaaggcgcg
     2401 taaagcgtcg agcggcttgc cggggcaggg cgcgaaagcc tcgcgctccc ataaggcgcg
     2461 gcggttgatc tcgcagcgat cgccatgggc gcgccagatg acgtccggat cgagagcgat
     2521 catcgaatag cggccgcgct gtgcgccgcc ttcgaccgat tccagcatga agacattgcc
     2581 ggcgcggccg gccgataatt tcaggaacgc cgagaccggc gtttcgagat cggcgaccag
     2641 ggtcgagacg gcgacgctgg cccgtccggc ctcgtagcgc cgcgcaattt cgtcatattc
     2701 ctggccggcg atcatgaaat cccgccgatg gcggtctgca gcaccgactg gttgatcgta
     2761 acgccgagct cgcgctgcag cgcggcgata tattcgccga acatgtcatt gaccagcgcg
     2821 gcgtcggcgc gctgctgcaa ttgcttggtt tcggcgctcg aaaaatcgac tggcggaatc
     2881 gtatccttgg tcaccttgaa gacgagccgg ccatcgccga cggtagccga gcctgcgccg
     2941 ctggacggcg cattgaaaat cgcggtcgtc acattttcgg caaggcctgc gccgccgctt
     3001 cggcggatat cggcggcgct cttcacgtcg agcttatccg cctcggcgag cgaggcgagc
     3061 gtcgccccat tgtcgagctt gatcaccatg tcggccgctt ttttcgagag cgccttggcg
     3121 acctcctcgt cgcgccactg cttggtcact tggtccttga cctcgtcgag ggtacggtcg
     3181 tgcggcggat cgatcttggt cacttcgaac cagacgaagc cgttatccgg cgtcgagacc
     3241 ggctgatcgt cgacgccgat atcggacgcg tagacggcgc ggagcacgtc cttctcgtcg
     3301 ggaatgccgg gcacggcctt gccgtcctta tcgagccctt ccgcgtcaat ggccgggatc
     3361 gcccgcacgt cgagaccgac ggtcttggcg gcctcggtca gcgtctttcc ggaagcgcgg
     3421 gcgtcctcga tcttgtcgtg aatcgacaaa gcctcgtcgg cggcgcgctg cgtcgccacc
     3481 tccttcttca tctcggcggc gacttcgtcg aaaggcttga cgctgctcgg cgtgattttc
     3541 gtcacatggg cgatgaccgg tccgaactgc cccttgacca cgtcgctgac gccgccgtcc
     3601 ggcagagcga aagccgcgtc ggcgaccgcc ttgtcgaaaa tcccggtctt ggtcgtctcg
     3661 ccgagatcga cgaacttgcc gcccgaatcg ctcgccttgg cgatctcgtc gaacggcgtt
     3721 ccgctcttga tcttcgcctc ggccgccgcg gccttggctt cgtccgaaaa gacgatctgc
     3781 tgcacgtcgc gcttttccgg cgtggtgaag cgctggtcct tgacgcgctc gtaaagcgcg
     3841 cgcgcgtcgg cgtcgctgac ttcatccggc ttggcgatgg tcgagggcgt caccgcgaga
     3901 agattgatcg cgcgatattc cggcgcccgg tagttcgcct tgcggtcgtt gaagaagctc
     3961 tgcaactgct ccggcgtcgg cgtcggaatg tcgcccgccg cgctcgtcgg caggatggcg
     4021 tagtcgaccg accggctttc cgagccgaaa tgagcgagcg cctcgacgag gactttcggc
     4081 gccgtcagat tgccggcgag cgacaggccg atctcctggc gaagcgagcg tttcctctgc
     4141 tcggcgaaaa agccgctctc gctcaagccg gattcgcgca aggcttcgtc gaaacgctgc
     4201 cgatcgaagc ggccggccgc gtcctgaaat tccggcgatt cgcggaccgc cttggcgatc
     4261 gtgtcgtccg atatggcgag gccgagcgag cgggcgcgcg cgtcgagagc cgcgtccgcg
     4321 atcatccggc cgagcacttg cgaatcgagc cccatcgcat gcgcttgggc gttggtgatc
     4381 ggcgccttca attggcgctg atattgctgg agcaggttct gatagtcccg ttgaaaggcc
     4441 tgggtcgaga tcgcctcgcc gccgacgctc gccacgtcgt tcttgccata gccttcgaac
     4501 ggattgcccg tgccccacca ggcgaagctc aaaatgatga cgccgagcac gatcgccatg
     4561 atcgcccggc cggcgaagcc ctgcgaagca gagcgcatga atccgagcat ggctcgttct
     4621 gatcctgatc ctttgcggcg aaagccgccg atttcgcccc tatctagcgg cgcctcgccg
     4681 ctttgcatag ccgcggcggg atgataatcg ccgcgcgaaa cgaaacgcgg cgaggcttga
     4741 aaatgacccc tggcgtgcgg cccctggtgg ccggcaattg gaaaatgaat gggctcaagg
     4801 cctcgctcgg cgaaatcgcg gcgatgcgca aggccgcgga ggccggcgcg gctggcgcgg
     4861 cggaactggc ggtctgcccg ccggcgacgc tgctcagcga ggcggccgcg ctcgtcggcg
     4921 gcgggcccgt tgcgctgggc gggcaggatt gccacgccaa agcgagcggc gcccataccg
     4981 gcgacatcgc cgccgccatg cttaaggacg ccggggcgtc ttacgtgatc gtcgggcatt
     5041 ccgagcgccg gaccgatcat ggcgagagcg acgcgctcgt gcgcgccaag gccgaggcgg
     5101 cgctcgccgc cggcctcgcc gcgatcgtct gcgtcggcga gacgcgggct gagcgcgagg
     5161 cgggcggcgc gatcagcgtg acgagccgcc agatcaaggg ctcgctgccg ccgctcgccg
     5221 atccggccca catcgtcgtc gcctacgagc cggtctgggc gatcggcacc ggcctgacgc
     5281 cgaccgcggc ggacgtggcc gaaatgcacg ccgcgatccg ctcgctgctc ggcgagattt
     5341 acgggcccgc cggcgcgacg atccgcatcc tctatggcgg gtcggtcaag ccggccaacg
     5401 cggccgaact cttgagcctc gccaatgtcg acggcgcgct ggtcggcggc gccagcctca
     5461 aagcggcgga ttttttgggc atagcgggcg tctatcgcgg cgcgtaaatc ccgtctcccc
     5521 gcttttgcgt cgccttgcgg ggagaagggt tacgcctgcg caaatcgctg ccgcgcgcgc
     5581 tcctgcgcct tggcggcttc gctggcgcgg tcgcgggtgg gcgcgttcgt ctggagcgag
     5641 gcgagcagac ggcgcgccgc cgcggcgaca tcctccaccg cctgatcgaa gacggcctga
     5701 ttggccttgg agggatgggc gaaaccgctc agcttgcgga caaattgcag cgccgaagcg
     5761 cgcacttctt cttcggtcgc tggcggttcg aaattgaaaa gggtgcggat gttgcggcac
     5821 atcgacctgt tctcgctttg aggcgccccg ctgcatccga ggcggtccgg caaggatagt
     5881 caaaagccgc taaaagcgaa atcgcccgcc gcgcatttcg cgcgcagatc gggcgaagat
     5941 acgtcgccgg gaggagcttt ggcccttaag cagcgagctt cgtccagcaa tgcgaggacg
     6001 agggagcata gcccgctccc ggcgaagtcg tccgtgaaat gccgctacgc tgcggtccgg
     6061 ccgaatacga cagcgatcgt tgcggcggta atgcccggtg gcgccatcga tacctccacg
     6121 ctagaggcga cgctgaagac gcgcgtggcg ctcgctcagc gcccatgacc aatagattgg
     6181 accgggaatt ttataagtca agcgcgcgcc gcaaaggccg cgttcaaatc ttgccccgat
     6241 tgccggagcg cgcttttgca gttcaggaaa acaacacgcc gccattcacc gcaacggtct
     6301 ggccggtcat gtacgcattg ccgaccagca tgagaaccgc ttgcgcgatc tcgtcgcccg
     6361 agccgaaccg gccgaccggg atgcgcgcgg cgaagccctg ttcgatcagc ggcctgccca
     6421 tatcggtttc gatcaggccc ggcgcgatgg cgttgacggt cacgccttcc ggcgcgaggc
     6481 gcgcggaata gccacgcgtc aagccttcga gcccggcttt ggaggcattg taggcgacgc
     6541 tgaccgcgcc ggcggcgcgc gccgcgaccg aggaaatatt aacgatgcgt ccccagcgcc
     6601 gcgcccgcat gcccggtagg acggcctgcg tgcacaagaa cgccgatttg agattgacgg
     6661 cgatggcgcg gtcgaaatcc tcttcaacaa tctcctcgat gctgcgcgcc gccgccatgc
     6721 cggcattgtt gatgaggatg tcgatcgggc cgagccgctc ttcgatttca tagaccatgt
     6781 tctgcacggc ggcgcgagac gaaacgtcgg cgccgatcgc cgtggcgcgg ccgccgccct
     6841 tatgaatcgc ctccacgacg gcggccgcct cgtcgctgcg ctcgcgataa ttcacagcca
     6901 aagccgcgcc tgctttcgcc aaggtcaagg cgatcgcgcg cccgatgccg cgcgagccgc
     6961 cggtgaccaa agcgacgcgg tttctcaatt ctgacatgcc gataccttta cggtgagagc
     7021 gagaggcgac gggacgcggc aaagcggcgt tgcaaaaaac gggcgcccgc tgtgcaagct
     7081 tagcggattt catgcgccgc agcagcgact atctcaagcg ctcttccgac aggccaggag
     7141 ctttaagatg tcgctcgaaa tcgcgctgac gttgatcggc ggtccgacgg ttctgatcga
     7201 aatggcgggc ctgcgcttcg tcactgatcc gacattcgat caaccgggcc tctatcaggg
     7261 cggggtgact ctggaaaaga ccaccgcgcc cgcgctttcc gcggagaaga ttggacgagt
     7321 cgacgcggtt ctgctcagcc acgatcagca tttcgacaat ctcgatcatg gcggccgcgc
     7381 cttcctcgcg caagcgcgga cgaccttcac gacgccggtc ggggccgcca gactcggcgg
     7441 caacgccgtc ggcctcgaag cttggcggag ccggaaattc gaaagcgaca aaggcgcggt
     7501 ccatgtcacc gccgcgccgg cgcgccatgg cccggtggga atcgaaccga tc
//
LOCUS       DATGJW010000440         4021 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_293405, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000440 DATGJW010000000
VERSION     DATGJW010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4021)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4021)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4021
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_293405"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            39..833
                     /locus_tag="VKU03_13150"
     CDS             39..833
                     /locus_tag="VKU03_13150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331575.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HLJ72261.1"
                     /translation="MWGIVPAAGRGSRIQPLAFSKELLPVGSRRDGATERPCAVSEYL
                     VERMIYAGVDRIAFVIGSGKYDIVSYYGAGYDAAAVAYVVQPSPAGLCDAVFRAAPML
                     DDEPVLIGLPDTIWFPKDGFAALPDDKLSFLLFPVARPELFDAVLLDGAGKVLSIEVK
                     KPGDPSRWIWGAFKMPASIFRALHALWLQRGGQDEYFGTLVNAYIAEGGEAVGVRAGQ
                     AYVDVGTLHGYRAALQLLSADHGESGEAISAVAISPPLGRAAAPIS"
     gene            848..1606
                     /locus_tag="VKU03_13155"
     CDS             848..1606
                     /locus_tag="VKU03_13155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR04290 family methyltransferase"
                     /protein_id="HLJ72262.1"
                     /translation="MRYSAEEIRQRVASLGPWFHNIDLDGVATAPDHFLGDYPSVKWS
                     KFADAVPADLSGKSVLDIGCNAGFYSIEMKRRGAARVLGVDSDEDYLRQARFAAEVAD
                     CDIEFRRLSVYDVGALGERFDIVLFMGVLYHLRHPLLALDLIREHVVGGLMVFQSMQR
                     GADGAAPVAEDYAFWDRSPFDRPDFPRLHFIEHRYAGDPSNWWAPNRACVEAMLRSAG
                     FEILSRPEEEVYICGAAATPAGGGAVYPAAGAHG"
     gene            1603..2532
                     /locus_tag="VKU03_13160"
                     /pseudo
     CDS             1603..2532
                     /locus_tag="VKU03_13160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013533207.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="beta-xylosidase"
     gene            2651..3403
                     /locus_tag="VKU03_13165"
     CDS             2651..3403
                     /locus_tag="VKU03_13165"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HLJ72263.1"
                     /translation="MAHPYDETIGLGGHLADFTDLIIVHVTDGAPRDMRDARAHGFAE
                     RSAYAQARRRELTAAVAEAGIDPSALLCLGVIDQEAPLCLAALSRRLAALFLRHRPLA
                     VFTHAYEGGHPDHDATAFAARAAIRLIADQGGSAPALIETPLYHGENGRIVTQTFAPT
                     PPVPGSAALLGPRAAAMKRAMLAHFRTQRQVLAQFQTVVERFRLAPDYDFTELPNRGD
                     LYYGRFPWSLDGASWRKLAAAALVELGLSPCR"
     gene            3394..>4021
                     /locus_tag="VKU03_13170"
     CDS             3394..>4021
                     /locus_tag="VKU03_13170"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ72264.1"
                     /translation="MPLTVLNVAYPFAPVGRDAVGGAEQVLSALDAALVESGHRSLVI
                     ACEGSQVAGVHLPVESERGPLDDLARRRAWARHRRAINNACNCFPVDLVHLHGVDFGA
                     YCPASGATLVTLHLPLSWYDPNSLRPARADLWLNGVSQSQTRSAPAEARLLPPIDNGV
                     DVEALAARCGKRRFVLFLGRLCPEKGAHIAIAAARRASLPLIIAGQTFR"
BASE COUNT          602 a         1390 c         1313 g          716 t
ORIGIN      
        1 gatttcgccg gcgccgcagc gactttggga gcgtgacgat gtgggggatc gtacccgccg
       61 ccggccgcgg cagccgcatt cagccgctgg ccttctcaaa ggagcttttg ccggtcggca
      121 gccgacgcga cggcgcgacg gagcggcctt gcgcggtcag cgaatacctg gtcgagcgga
      181 tgatctatgc cggcgtcgac cggatcgcct tcgtgatcgg atccggcaaa tacgacatcg
      241 tcagctacta cggcgcgggc tatgacgccg ccgcggtcgc ctatgtcgtt cagccctcgc
      301 cggccggcct ctgcgacgcc gttttccgcg ccgcgccgat gctcgacgac gagcctgttc
      361 tgatcgggct ccccgatacg atctggtttc ccaaggatgg gttcgcggcg cttccggacg
      421 acaaactgtc gttcctcctt ttcccggtcg caaggccgga actgttcgat gccgttcttc
      481 tcgacggcgc cggcaaagtc ctctcgatcg aggtcaagaa acccggcgac ccctcacgct
      541 ggatttgggg cgccttcaaa atgccggctt cgatctttcg tgcgctgcat gcgttatggc
      601 tccagcgcgg cggccaagac gagtatttcg gcacgctcgt caacgcctat atcgccgagg
      661 gcggcgaggc ggtcggcgtc cgcgccggac aggcctatgt cgacgtcggc acgctgcacg
      721 gctaccgcgc cgcgctgcaa ctcctcagcg ccgatcacgg cgaaagcggc gaggcgatct
      781 ccgccgtcgc catctcgccg ccgctcgggc gcgccgccgc gccgatctct tagccgggag
      841 cctgccgatg cgctattcgg ctgaagagat acgccagcgc gtcgcctcgc tcgggccctg
      901 gttccacaac atcgatctcg acggcgtggc gacggcgccc gatcacttcc tcggcgacta
      961 cccttcggtg aaatggagca aattcgccga cgccgtgccg gcagatctca gcggcaagag
     1021 cgtgctcgac atcggctgca acgccggctt ctactcgatc gagatgaagc ggcgcggcgc
     1081 ggcgcgcgtg cttggcgtcg atagcgacga ggattacctg cggcaggcgc gcttcgcggc
     1141 cgaggtcgcc gactgcgaca tcgagttccg gcgcctctcg gtctacgacg tcggcgcgct
     1201 cggcgagcgc ttcgacatcg tgctgttcat gggcgttctc tatcatttgc gccatccgct
     1261 gctggcgctc gatctgatcc gcgagcatgt cgtcggcggc ctgatggtgt tccaatcgat
     1321 gcagcgcggc gcggacggcg ccgctccggt cgccgaggat tacgccttct gggaccgctc
     1381 gcccttcgac cggccggatt tcccccgcct tcatttcatc gagcatcgct acgccggcga
     1441 tccgagcaat tggtgggcgc cgaacagagc ctgcgtcgag gcgatgctga gaagcgccgg
     1501 gttcgaaatc ctctctaggc cggaggagga ggtttacatt tgcggggccg cggcgacgcc
     1561 ggcgggcggc ggcgcggtct atccagcagc gggagcgcac ggatgatcga agcggcgatg
     1621 atctggaacg agccgaacaa caaatcgcat tgggatcccg agatcgaccc cgactggcgg
     1681 ctcttcgcgg agatgacgat cgccgcggcg gacgcgatcg ccgcagaaaa tccctgcctt
     1741 cccaaagtgc tcggcggcat gtcgccgatc gatccgcttt tcgtcgccaa tatgaagggc
     1801 cgcggcgtgc tcgaccacct cgacgccgtc gccgtgcatg gcttccggct cgattggaat
     1861 ctatggcagc tcaaggaatg gccggccaaa atcgaggaaa ttcaggcggt caccgatctc
     1921 ccggtctggg tcagcgaggt cggcgtctcc acattcggcg ccgacgaagt ccaggactgg
     1981 ggactggcgc ggacggcgga gctgctgatc ggcaaggcgc cgcgcgtcca ttggtacagc
     2041 ctctacgatc tgccgcgcac ctaggaggcg acgacgcgcc accgcgaggc ggagggctcg
     2101 tcgtactacc ggcatttcgc gatgggtctt ttgcgcgagg acgggacgcc gaagccggcg
     2161 ctcgatcgct tcgcgcgact gacgccagcc cttggtcttt gtcaatggtt ccacttcgag
     2221 gatcaccggc tcgatgaggc cgtcgcctgg atgaagcggc tcggcgtcgc gtatctgaga
     2281 acggggctgt cgtgggccga cagttttcgg ccaaacgcgc tcgcctggtt cgatcggcag
     2341 atgcaagcgc tcgaggattt cgacgtgacc gtcacgttct gcttcacgcc ggcgcatcgc
     2401 ggcctcgccc cgcatcacac aagcgcgcca atcgtaaagg aggagttcgc cgaattctgc
     2461 gccgcgatga tccgccgcta cgcgccggcg aagccggccg ccctgcctcg ccgcctcgtg
     2521 gcgacggcct gaagccgggc ttcgttcgat ggcgagcgcg gcgccacaat ttcggagcga
     2581 cggcagcccg ccaagcctgt ttcaggcgct cgacttatgc gccgccgaag gccgccaagc
     2641 gatggtggtc gtggctcatc cctacgacga gacgatcggc ctcggcggac atctcgcgga
     2701 tttcaccgat ctcatcatcg tgcatgtgac cgacggcgcg ccgcgcgaca tgcgggacgc
     2761 ccgagcgcat ggcttcgcgg agcgcagcgc ctatgctcag gcgcgtcggc gggagttgac
     2821 cgccgccgtt gcagaagcag gcattgatcc ttccgcgctg ctctgtctcg gcgtcatcga
     2881 tcaggaggcg ccgctttgcc tcgccgcttt gtcgcgccgc cttgccgcgc tatttctgcg
     2941 ccaccggcca ctcgccgtat tcacccatgc ctacgaaggc ggccatcccg atcacgatgc
     3001 gacggctttc gccgcgcgcg ccgccattcg gctcatcgcg gatcaaggcg ggagcgcgcc
     3061 ggcgctgatc gagacgccgc tctatcacgg cgaaaacggg cggatcgtca cgcagacctt
     3121 tgcgccgacg ccgccggttc caggatccgc ggcgctgctc ggcccgcgcg ccgcggcgat
     3181 gaagcgcgcg atgctcgcgc attttcgtac ccagcgacag gtcctcgcgc aattccaaac
     3241 cgtcgtcgag cggttccgcc tcgcgccgga ctacgacttc accgaattgc cgaaccgtgg
     3301 cgacctctac tacgggcgct ttccctggag cctggacggc gcctcgtggc ggaagctcgc
     3361 ggccgcggcg ctcgtcgagc ttggcctgtc gccatgccgc tgaccgtcct caacgtcgcc
     3421 tatccattcg cgccggtcgg ccgcgacgcc gtcggcggcg ccgagcaggt gctgagcgcg
     3481 cttgacgcgg ctttggtgga aagcggacac cgatccctgg tgatcgcctg cgaggggtcg
     3541 caagtcgccg gcgttcacct gccggtcgag agcgagcgcg gcccgctcga cgatctggcg
     3601 cggcggcggg cttgggcgcg gcaccgccgc gccatcaaca atgcctgcaa ttgctttccg
     3661 gtcgatctcg tgcatctgca tggcgtcgac ttcggcgctt actgccccgc gagcggcgcg
     3721 acgctcgtca ccctgcatct gccgctctcc tggtacgatc cgaactccct gcgcccggct
     3781 cgcgccgatc tctggctcaa tggcgtttcg caaagccaga cccggagcgc tccggccgag
     3841 gcgcgcctgc tcccgccgat cgacaacggc gtcgatgtcg aagcgctcgc cgcgcgctgc
     3901 ggcaagaggc ggttcgtcct tttcctcggc cggctgtgcc ctgaaaaagg cgctcatatc
     3961 gccatcgccg cggcgaggcg cgcttcgctt ccgttgatca tcgccggcca aaccttccgc
     4021 t
//
LOCUS       DATGJW010000441         8455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_495321, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000441 DATGJW010000000
VERSION     DATGJW010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8455
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495321"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(250..744)
                     /locus_tag="VKU03_13175"
     CDS             complement(250..744)
                     /locus_tag="VKU03_13175"
                     /inference="COORDINATES: protein motif:HMM:NF024675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioesterase family protein"
                     /protein_id="HLJ72265.1"
                     /translation="MTPLHVLDIEVPSDWIDYNGHMNDGCYAIAFSRGGDAFMEEIGL
                     GAKKREATKRSIFTLALVIRFIAEAKRGERLAISVQLLECDKKRMRFWLEARKEGVLI
                     ATSEQVLTCVDLSGERPRAAEFPPDVAARLEKLAADHAELPTPLEAGQGLTLKKRATF
                     SVTP"
     gene            919..1833
                     /locus_tag="VKU03_13180"
     CDS             919..1833
                     /locus_tag="VKU03_13180"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ72266.1"
                     /translation="MQGGGAARRRGLVLTSVATLIWSTAGVFTRLLSHLDLWTLLAGR
                     AGFGALFLGAAALLEWRRGRLGPFFGLGSKLAPLIIFLAAVAMSSYIAAVRVTTVADV
                     MVIYSTLPFVTAGLAFLLLGERPSGRTLAASGLALAGVAVMVGGAAGTGRFVGEGLSF
                     LMTVSFGLMVVLQRRHPEMSLTSINTIGSALAAIVAFSLAPRQAVGARDLVILAVFGL
                     ATVCIAFVMFMNGAKLIPAAESALINMAEAVLGPLWVFAFFGEDPGASAVGGGALVLG
                     AVLWRIAPDLWRARAGVEPADVGAPPLL"
     gene            1870..2625
                     /locus_tag="VKU03_13185"
     CDS             1870..2625
                     /locus_tag="VKU03_13185"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333920.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72267.1"
                     /translation="MAEEKYALVTGAGVGIGRAASLALAKAGWNIALTGRRSPPLEET
                     AGAVKSLGRKALVHPCDVGDPQAVKALFAKIAETFGRLDLLFNNAGAGAPGVPMEDLT
                     YEQWKAVVDVNLTGAFLCAQGAISIMKSQTPKGGRIINNGSISAHAPRPNSAPYTATK
                     HAITGLTKSIALDCRKYDIACGQIDIGNALTDMARKMTQGVPQADGRIAIEPTIDADE
                     VGQAIAHMASLPLTTNIFTMTIMATKMPYVGRG"
     gene            complement(2669..3550)
                     /locus_tag="VKU03_13190"
     CDS             complement(2669..3550)
                     /locus_tag="VKU03_13190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008598938.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ72268.1"
                     /translation="MDWDNVRVFLAVARAGQFVAAAKRLRLDHATVSRRIAALEASLG
                     ARLFDRRTTGAKLTAAGERFLSAAEQMESAFLHAQSELGDKDVEMTGIVRIGAPDGFS
                     TYYLAHIFGDFLARHPGVKIQLVPIPQVMPLARREVDIVVVLDKPEAGRFFARKLTDY
                     SLGVYASRDYLARLGAPNDVAELSTRSLIGHVEEHAFSSALDYVRDLFDGAPTAFECA
                     SVVTQIEALRAGLGLGVVHNYIAHRFDDLQRVLPDRRAERSYWIVTHEDTRGLRRIRA
                     AHEHLVACVERDRKMFA"
     gene            3617..5728
                     /gene="ligA"
                     /locus_tag="VKU03_13195"
     CDS             3617..5728
                     /gene="ligA"
                     /locus_tag="VKU03_13195"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009031140.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HLJ72269.1"
                     /translation="MPRKPAATPFVVPQSFAEAKAKHAELSAEIAKHDKLYYADDAPI
                     ISDAEYDELRKANNAIEAQFSELVGADSPSVRVGAAPSARFAKIRHAVPMLSLDNAFS
                     EEDVREFVGRIRRFLRLADDAEIAFSAEPKIDGLSMSLRYEGGALVSGATRGDGAVGE
                     DVTANIKTLADVPKTLKGKSVPAVCEVRGEVYMSKRAFIELNKRQAEIGGQVFANPRN
                     SAAGSLRQKDPSITASRPLGFFAYAWGEMSAMPAATQSGMIKWFESCGFKTNPLTRMC
                     RSVESLLEFHREIEAQRATLDYDIDGVVYKIDRLDWQERLGYVSRNPRWAIAHKFPAE
                     KATTLVKDIEIQIGRTGALTPVAKLEPVTVGGVVVQNATLHNEEEIKRLDVRVGDTVT
                     IQRAGDVIPQVLGVVAEKRPRGAKPYEFPKKCPCPLHTDVVRETIAGGAEGARAHCTG
                     EFACPYQTIEHLKHFVSRRAFDIDGLGEKQIEFFYEQGWVKEPADIFTLEARDKDIRL
                     EEVEGFGETSVRNLFAAIAARRDIPLERFIYALGIRHVGETTALALARGYGSWDALHA
                     ACMKIVKGDEEAEQEMDALDQIGDTVIESLKDYFGEEHNRRRVERLAKEVRIRDAEKP
                     RADHALAGKTVVFTGSLEKMTRDEAKAAAERLGAKVSGSISKKTDLVVAGPGAGSKLA
                     KARELGVETIDEEGWLRRIGA"
     gene            5943..6836
                     /locus_tag="VKU03_13200"
     CDS             5943..6836
                     /locus_tag="VKU03_13200"
                     /inference="COORDINATES: protein motif:HMM:NF014376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AraC family transcriptional regulator"
                     /protein_id="HLJ72270.1"
                     /translation="MQCLTQADLIATAEHDRRDRAAFWRNPRYDGLECLSATFFTHRF
                     PRHTHDTYVMGIVHAGCNAYSQGGVKIYAGAGDICVINPGVVHDGGAFDQGFSYRMTY
                     PSIALMKELAEDVTGRPVHRAPSFKAPIMRDDEVNALFRSAHERMETGAETVGADENL
                     IAAYGLMLLRYADLDPADAIGREPERIGRVRAFLEENFSAPVDLKTLAEIAGLSRFQL
                     LRAFRRETGMTPHAYLVDCRVRAARRLLARGETIAEAALACGFSDQSHLTRLFKAHAG
                     LTPARYRGARNFVQDGGAAGH"
     gene            6977..7657
                     /locus_tag="VKU03_13205"
     CDS             6977..7657
                     /locus_tag="VKU03_13205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:1902495 - transmembrane transporter
                     complex [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AzlC family ABC transporter permease"
                     /protein_id="HLJ72271.1"
                     /translation="MRKEMADGVRDILPAMIAGAPIALLYGALAVGKGMTPVEASLSS
                     FLIFAGGAQFAALELWRSPPPILALVFSTLLINARHILMGASLRSKLGKFSPLQRAFG
                     LFFMADENWAMSERRAAARVLTPVYYLAMSVVFWANWSLWTTLGAIGGAFLGDPKRIG
                     ADFAFTALFIGLVAGFWRGRSTAVTVAASAIASAAAYRVLGPPWHVAAGALCGVGAAY
                     LYARRAKA"
     gene            7654..7971
                     /locus_tag="VKU03_13210"
     CDS             7654..7971
                     /locus_tag="VKU03_13210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008547628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AzlD domain-containing protein"
                     /protein_id="HLJ72272.1"
                     /translation="MTSSLAIDPLTLIAILAMALTTYGTRIAGLFLTLHHAPSAPMQA
                     ALDAIPPAVLIAVVAPMMLATGWPETIATALTALAATRLPLVATVAFGAACVVALRLA
                     LGG"
     gene            8068..>8455
                     /locus_tag="VKU03_13215"
     CDS             8068..>8455
                     /locus_tag="VKU03_13215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HLJ72273.1"
                     /translation="MSAAFLMTSLIIVVSPGTGVLYTLAAGLSRGWRASIVAAFGCTL
                     GIVPHMAAAILGLSALLHESALAFRAFKYLGVAYLLFMAIRTLREHGPLKLETKPGTR
                     SSFQTIVSAILVNLLNPKLSIFFFAFL"
BASE COUNT         1394 a         2805 c         2763 g         1493 t
ORIGIN      
        1 cgaccgcgaa gcggcttgac cttgacgcgc ctccgcgtcg atggcagcgg ggcgttttga
       61 gatttgcgtc atgcgcgcct gtaaggttcg aaaaagcctt gagcgctgcg cctgcttttt
      121 tctgagtctc gacaacagaa attcggcgag gaaatcccga aacgttcttg ttttgcgata
      181 tcgccgcctg aagcgactcg gttcatgctc attttcccgg acagccctgc gctgcgcaga
      241 gcgagggact taaggcgtca ccgaaaacgt cgcccttttc ttcagcgtca gtccctgccc
      301 ggcctccagc ggcgtcggca attccgcgtg gtccgccgcg agcttttcga gcctcgcggc
      361 gacgtcaggc gggaattccg ccgcccgcgg ccgctcgccg gaaaggtcga cgcaggtcag
      421 cacttgctcg ctggtcgcga tcagaacgcc ctccttgcgc gcctccagcc agaagcgcat
      481 gcgcttcttg tcgcattcca gcaattggac gctgatcgcg aggcgctcgc cgcgcttcgc
      541 ctcggcgatg aagcgaatga caagggcgag ggtgaagatc gagcgcttcg ttgcttcgcg
      601 ctttttggcg ccgagcccga tctcctccat gaaggcgtcg ccgccgcggc tgaacgcgat
      661 ggcgtagcag ccgtcgttca tatggccgtt atagtcgatc cagtccgacg gcacttcgat
      721 gtccagaacg tgcagcggcg tcatcgcgct cctccttgtc gacgcgagtt taacagacgc
      781 cggaaggccg gaagagaggc atcggccggg cgctacccga ttttggcatg acggcaatga
      841 aagcctcggc ttgacgcgag gccccgcgac ctgacgcaat gcggctcagc agcgattcga
      901 actcctcaag gagagcccat gcagggcgga ggcgcggcgc gccggcgcgg gctcgtcttg
      961 accagcgtgg cgacgctgat ctggagcacg gcgggcgttt tcacccgctt gctcagtcat
     1021 ctcgacctct ggacgctgct cgccggccgc gccggcttcg gcgcgctgtt cttaggggcg
     1081 gccgccctgc tggaatggcg acgcggccgg ctcggcccgt ttttcggcct gggctccaag
     1141 ctcgcgcctt tgatcatctt tctcgccgcg gtcgcgatgt cgtcctatat cgccgcggtc
     1201 cgcgtgacga ccgtcgccga cgtgatggtc atctattcga cgctgccgtt cgtcaccgcc
     1261 ggcctcgctt tcctcttgct cggcgaacgc ccctccggcc ggacgctggc ggcttccggc
     1321 ttggcgctgg ccggcgtcgc cgtgatggtt ggcggcgcgg cggggaccgg ccgattcgtc
     1381 ggggaaggcc tttccttcct gatgacggtc agtttcggcc tgatggtcgt gctgcagcgc
     1441 cgccacccgg aaatgtcgct gacctccatc aacacgatcg gctccgcgct ggccgcgatc
     1501 gtcgccttct ctttggcgcc gcgccaggcg gtcggcgccc gcgacctcgt catccttgcc
     1561 gttttcggcc tcgccacggt ctgcatcgcc ttcgtcatgt tcatgaacgg cgccaagctc
     1621 atcccggccg ccgaatcggc cttgatcaac atggccgagg ccgtgctcgg cccgctctgg
     1681 gtcttcgcct ttttcggcga agatcccggc gccagcgccg tcggcggcgg cgcgctggtg
     1741 cttggcgcgg ttttgtggcg gattgcgccg gatctctggc gggcgcgcgc cggcgtcgag
     1801 ccggctgacg tcggcgcgcc gcctttgtta tagaaaggcc gcgcgtcgtg gcgcggcagg
     1861 gaggatgtaa tggcggaaga aaaatatgcg ctggtcaccg gcgccggagt cggcatcggc
     1921 cgcgccgcct cgctggcgct cgccaaagcc ggatggaata tcgcgctgac cgggcgtcgg
     1981 tcgccgccgc tcgaagagac cgccggcgcc gtcaaatcgc tcggccgcaa ggcgctggtt
     2041 catccttgcg atgtcggcga tccccaggcg gtcaaggcgc tgttcgcgaa aatcgcggaa
     2101 acgttcggcc ggctcgatct cctcttcaac aatgccggcg ccggcgcgcc cggcgtgccg
     2161 atggaggact taacctacga gcaatggaaa gcggtggtcg acgtgaattt gaccggcgcc
     2221 tttctctgcg cgcagggcgc gatctcgatc atgaaaagcc agacgccgaa aggcggccgg
     2281 atcatcaaca acggctcgat ctcggcccat gcgccgcggc cgaactccgc gccctatacg
     2341 gcgaccaaac atgcgatcac cggcctcacc aagtcgatcg cgctcgattg ccgcaaatac
     2401 gacatcgcct gcggccagat cgatatcggc aacgcgctga ccgacatggc gcgcaagatg
     2461 acgcaaggcg tgccgcaagc cgacggccgc atcgccatcg agccgacgat cgacgccgac
     2521 gaagtcggcc aggcgatcgc gcatatggcg agtctgccgc tgacgacgaa tattttcacg
     2581 atgacgatca tggcgaccaa gatgccgtat gtcgggcggg gctgatccct tctccccgcc
     2641 ttgcgtcgca aagcggggag aagcgatttt acgcgaacat cttgcggtcg cgctcgacgc
     2701 aggcgacgag atgctcgtgc gcggcgcgga tgcggcgaag gccgcgcgtg tcctcgtggg
     2761 tgacgatcca gtagcttctt tccgcccgcc ggtccggcag gacgcgctgc aaatcgtcga
     2821 agcggtgcgc gatgtaattg tgcacgacgc cgaggccgag cccggcgcgc aaggcttcga
     2881 tctgggtgac gacgctggcg cattcgaaag ccgtgggcgc gccgtcgaaa agatcgcgca
     2941 cgtaatcgag cgccgaggag aaggcgtgct cctcgacatg gccgatcagg ctgcgggtgg
     3001 aaagttccgc aacgtcattc ggcgcgccga ggcgggcgag atagtcgcgg ctggcgtaga
     3061 cgccgagcga ataatcggtg agcttgcggg cgaagaagcg gccggcttcg ggcttgtcga
     3121 gcacgacgac gatgtcgacc tcgcgccgcg ccagcggcat gacttgcggg atcggcacga
     3181 gttggatctt gacccccgga tggcgggcga gaaagtcgcc gaaaatatgg gcgagatagt
     3241 aggtcgagaa gccgtcgggc gcgccgatcc gcacgatccc ggtcatttcg acgtccttgt
     3301 cgccgagctc gctctgcgca tgcagaaagg cgctctccat ctgctcggcg gcgctcagga
     3361 agcgctcgcc ggcggcggtg agcttggcgc cggtggtgcg gcggtcgaaa agccgcgcgc
     3421 cgaggctcgc ttcgagcgcg gcgatgcggc ggctcaccgt cgcatggtcg agccgcagcc
     3481 ttttcgccgc cgcgacgaac tgcccggccc gcgcgaccgc cagaaagacg cggacattgt
     3541 cccagtccac ttgctgaccc cggctggcgc ttttacacga cttccaccgt gtcgaacgcc
     3601 gcgataaact cctagcatgc ctcgaaagcc tgccgctacg ccctttgtcg tcccgcaaag
     3661 tttcgcagaa gctaaagcaa agcatgcgga actttcggcg gaaatcgcca agcatgacaa
     3721 actgtactat gctgacgacg cgccaataat ttccgacgcg gagtacgacg aacttcggaa
     3781 agccaataac gcgattgaag cgcaattctc cgagctcgtc ggcgcggatt cgcccagcgt
     3841 tcgcgtcggc gccgcgccct cggcgcgctt cgccaaaatc cgccatgcgg ttccgatgct
     3901 gtcgctcgac aacgcgttca gcgaagagga cgtgagggaa ttcgtcggcc gcatccgccg
     3961 cttcctgcgc ctcgccgacg atgcggagat tgcgttttcc gccgagccga agatcgacgg
     4021 cctatcgatg tcgctgcgct acgaaggcgg cgcgcttgtc tccggcgcga cgcgcggcga
     4081 tggcgcggtc ggcgaggacg tcaccgccaa catcaagacg ctggccgacg tgccgaagac
     4141 gctgaaaggc aaaagcgttc cggcggtctg cgaggtgcgc ggcgaagtct atatgagcaa
     4201 gcgcgccttt atcgagctga acaagcgcca ggcggagatc ggcggccagg ttttcgccaa
     4261 tccgcgcaat tcggcggccg gctcgctgcg ccagaaggac ccttcgatca ccgcgtcgcg
     4321 gccgctcggc tttttcgcct atgcctgggg cgagatgagc gccatgccgg ccgcgacgca
     4381 atccggcatg atcaaatggt tcgagagctg cggcttcaag accaacccgc tgacccggat
     4441 gtgccgctcg gtcgagtcgc tgctggagtt ccaccgcgag atcgaagcgc agcgcgcgac
     4501 gctcgactac gacattgacg gcgtcgtcta caagatcgac cgtctcgatt ggcaggagcg
     4561 actcggctat gtctcgcgca atccgcgctg ggcgattgcg cataaattcc cggccgagaa
     4621 ggcgacgacc ctggtcaagg atatcgagat tcagatcggc cgcaccggcg cgctgacgcc
     4681 ggtcgccaag ctggagccgg tgacggttgg cggcgtcgtg gtgcagaacg cgacgctgca
     4741 caatgaagaa gagatcaagc ggctggacgt gcgcgtcggc gatacggtga cgatccagcg
     4801 cgccggcgac gtgatcccgc aagtgctcgg cgtggtggcg gagaagcgcc cgcgtggcgc
     4861 caaaccttac gaattcccga agaaatgtcc ttgtccgctc cataccgatg tggtgcgcga
     4921 aaccatagcc ggcggcgccg agggggcgcg cgcgcattgc accggcgagt tcgcctgccc
     4981 gtaccagacg atcgagcatc tcaagcattt cgtctcgcgc cgcgcctttg acattgacgg
     5041 gcttggcgaa aagcagatcg agttctttta cgagcaaggc tgggtcaagg agccggcgga
     5101 tatttttacc ctggaggcgc gcgacaagga catccgcctg gaagaggtcg agggattcgg
     5161 cgaaacctcg gtgcgcaacc ttttcgccgc catcgccgcg cgccgcgaca ttccgctcga
     5221 acgcttcatc tacgcgctgg gcatccggca cgtcggggag acgacggctc tggcgttggc
     5281 gcgcggctat ggctcctggg acgctcttca cgcggcgtgc atgaaaatcg tcaagggcga
     5341 cgaggaggcc gagcaggaga tggacgcgct cgatcagatc ggcgacacgg tgatcgaatc
     5401 gctgaaggat tatttcggcg aggagcacaa ccggcggcgc gtcgagcgcc tcgccaaaga
     5461 ggtgcgcatc cgcgacgccg aaaagccgcg cgccgaccat gcgctcgccg gcaagaccgt
     5521 cgtcttcacc ggctccctgg agaagatgac ccgcgacgaa gccaaggccg ccgccgagcg
     5581 gctcggcgcc aaagtgtccg gctcgatctc gaagaagacc gacctcgtcg tcgcgggccc
     5641 cggcgccggc tcgaaactcg ccaaggcgcg cgagcttggg gttgagacga tcgacgagga
     5701 gggatggttg aggcggatcg gagcgtgagg gagtcccttt cctcccccgc gaaagcgggg
     5761 gaccatccga agcatggtga aggaggaggg ctccggtctc ttaaggataa atcccaaccg
     5821 ccgcgacccc ctccaccgcg ctgacgcgcg gtcccccttc ccggtttcgc ttcgctccac
     5881 gggggaggaa agggcgcaat tccgttcaag acgccttggc cttctcccgc tagactgcgc
     5941 ccatgcaatg cctcacccag gccgatctca tcgcgaccgc cgagcatgac cggcgcgacc
     6001 gcgccgcctt ctggcgcaat ccgcgctatg acgggctcga atgcctgtcg gcgacctttt
     6061 tcacccatcg ctttccgcgg cacacccacg acacctacgt gatggggatc gtccatgccg
     6121 gctgcaacgc ttacagccag ggcggcgtca aaatttatgc gggcgcgggc gacatctgcg
     6181 tgatcaatcc tggcgtggtc catgacggcg gcgctttcga ccaaggcttt tcttatcgca
     6241 tgacctatcc gtccatcgcg ctgatgaaag agctggcgga agacgtgacc ggccgaccgg
     6301 tccatcgcgc gccgagtttc aaggcgccga tcatgcgcga cgacgaagtg aacgctctgt
     6361 ttcgctccgc ccatgagcgg atggagacgg gcgcggagac agtcggcgcg gatgaaaacc
     6421 tcatcgccgc ctatggcctg atgctgctgc gctacgccga tctcgatccg gcggacgcca
     6481 tcgggcgcga gccggagcgg atcggccgcg tgcgcgcctt tctcgaagag aatttcagcg
     6541 cgcccgtcga tctcaagacg ctcgccgaaa tcgccggcct gtcgcgcttc caattgctgc
     6601 gcgccttccg ccgcgagacc ggcatgacgc cgcacgccta tttggtcgat tgccgcgtgc
     6661 gggcggcgcg ccgccttttg gcgcgcggcg agacgatcgc cgaggcggct ttggcctgcg
     6721 gcttttccga tcagagccat ttgacccgcc tcttcaaggc ccatgccggg ctgacgcccg
     6781 cccgctatcg cggcgcgcgc aatttcgtgc aagacggcgg cgccgccggg cattagcacg
     6841 ctgaccccat gtcatccccg cggaaagcgg gaatccagtc cagcttatcc ggcaagcgcg
     6901 gagctccctg gattgcagct ttcgagggaa tgacaacgac gcgaacggta cgcggcgaac
     6961 attcatggcg ggcggcatgc ggaaggaaat ggcggacggc gtccgggaca tcctgccggc
     7021 gatgatcgcc ggcgcgccaa tcgcgctcct ctatggcgcg ctggcggtcg ggaaaggcat
     7081 gacgccggtc gaggcgagcc tatcctcgtt tctgatcttc gccggcggcg cgcaattcgc
     7141 cgctttggag ctctggcgca gcccgccgcc gatcctcgcg ctcgttttct caacgctgct
     7201 gatcaatgcc cggcacatcc tgatgggcgc gtcgctccgg tcgaagctcg gaaaattctc
     7261 gccgctgcaa cgcgcgttcg gcctcttctt catggccgac gagaattggg cgatgtcgga
     7321 gcgccgcgcg gcggcgcgcg tattgacgcc ggtctactac ctcgcgatga gcgtcgtctt
     7381 ctgggccaat tggagcctat ggacgacgct cggcgcgatc ggcggcgcct tcctgggcga
     7441 cccgaaacgg atcggcgcgg atttcgcttt caccgcgctg ttcatcggcc tcgtcgccgg
     7501 cttctggagg gggcgctcga ccgccgtcac cgtcgcggcg agcgcgatcg cctcggccgc
     7561 ggcgtatcgc gtgctcgggc cgccttggca tgtcgcggcc ggcgcgctct gcggcgtcgg
     7621 cgccgcctat ctttacgcgc gacgggcaaa agcatgacga gctcgctcgc gatcgatccg
     7681 ctgacgctca tcgccattct cgccatggcg ctgacgacct atgggacgcg gatcgccggc
     7741 ttgttcctaa cgctccatca cgcgccgagc gcgccgatgc aggcggcgct cgacgccatt
     7801 ccgcccgccg tgctgatcgc cgtggtcgcg ccgatgatgc tggcgaccgg ctggcccgag
     7861 acgatcgcca cggcgctcac cgcgctcgcg gcgacgcgcc tgccgctggt cgccaccgtc
     7921 gccttcggcg cggcctgcgt cgtggcgctg cgcctcgcct taggcgggta gcgccctggg
     7981 tgttcttcgc gactcgttcg tgtcccttca atcttaacac gaaggacacg aggagccgcg
     8041 cgaaggccgc aaaccagggg agagacgatg agcgccgcat tcctgatgac ctcgctgatc
     8101 atcgtcgtct cgcccgggac aggcgtgctt tacacgctcg cggccggcct ctcgcgcggc
     8161 tggcgggcga gcattgtcgc cgcgttcggt tgcacgctgg ggatcgtgcc gcatatggcg
     8221 gcggcgattc tcggcctttc tgcgctgctg cacgaaagcg ccctggcctt ccgagccttc
     8281 aaatatctcg gcgtcgccta cctgctcttc atggcgatcc gcacgctccg cgagcacggc
     8341 ccgctgaagc tggagacgaa gcccggcacg cggagctcct tccagacgat cgtctccgcc
     8401 atcctcgtca acctcctcaa cccgaaactg tcgatcttct tcttcgcgtt tcttc
//
LOCUS       DATGJW010000442         3760 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_707145, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000442 DATGJW010000000
VERSION     DATGJW010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3760)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3760)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3760
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_707145"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..162
                     /locus_tag="VKU03_13220"
     CDS             <1..162
                     /locus_tag="VKU03_13220"
                     /EC_number="3.4.23.36"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004190 - aspartic-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase II"
                     /protein_id="HLJ72274.1"
                     /translation="AVADFCDFHIGGWHWYVFNIADTAIVVGVALLAYDGLFAEKRVQ
                     AGQADASRP"
     gene            357..1016
                     /locus_tag="VKU03_13225"
     CDS             357..1016
                     /locus_tag="VKU03_13225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72275.1"
                     /translation="MTFRGSHRSLLLAAAVAAGLAAPVAARAGEDGQQPLWEGLGDMI
                     GLTGTDKVDKPIKYGERPKLVLPKTTDLPPPSPALSLTADWPHDPDVEKIKREKEAIL
                     HRHQRSPSLDKASLYGRPLSPDLLRSDHAAPGTLGAADHCQIDPRHCHWIRPDILEKL
                     GLKKEEDTVVAGQEPDRDWLTDPPKGYRVPAANTKATFEAADHADHGDQRYDLYKPPS
                     Q"
     gene            1133..1528
                     /locus_tag="VKU03_13230"
     CDS             1133..1528
                     /locus_tag="VKU03_13230"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLJ72276.1"
                     /translation="MKRFFKPARIAWSAAGQAHGVAHGPVFKRLLIAGQPGVDETGEV
                     PADLEAQIGAAFDNLLSVVEAGQMAAEDIVRIIAYVAAPRAYEMFERVRERKLGGLTP
                     AASYVETSGFSDPRWRVLIEGEAVRDQTA"
     gene            1542..1955
                     /locus_tag="VKU03_13235"
     CDS             1542..1955
                     /locus_tag="VKU03_13235"
                     /EC_number="3.1.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634320.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HLJ72277.1"
                     /translation="MTLEKDDSEAGPRGELTVRLIAMPGDTNANGDIFGGWVLSQMDQ
                     AGGIAGVERAQGRVVTIAVEAMTFIRPVRVGDVLCVYTEVERVGRTSMRIHIEAWAQR
                     FRTHFREKVTDAVFTFVAVDDEGRPRPIPREESQR"
     gene            2057..3166
                     /locus_tag="VKU03_13240"
     CDS             2057..3166
                     /locus_tag="VKU03_13240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015934037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HLJ72278.1"
                     /translation="MAGAGAVQDSLEWSRSRFESFDSMEGLAASEASAPPRFLSLLPR
                     GKLVHAVYPGFLVAATIALASSWLAQHYRAPVMLFALLFGMAFHFLHEEGRCVAGIEF
                     SSRTVLRLGVALLGARITLAQIASLGVLPVATVIVGVATTISIGVLLARRLGLRSSFG
                     ALSGGSVAICGASAALAIASVLPRHEDSERDAILTVVAVTALSTLAMILYPIFVVAIG
                     LDHVHAGIFLGGTIHDVAQVVGAGYTVSQETGDIATYVKLLRVAMLLPAVLAISFALT
                     RAGAARAATPALPGFLIGFALLVVVNSSGFLPKIAADLMSDVSRWCLVAAIAALGMKT
                     SFKNLFAVGWRPIALMLCETLWIAGLVLASVLLAA"
     gene            3258..3671
                     /gene="mscL"
                     /locus_tag="VKU03_13245"
     CDS             3258..3671
                     /gene="mscL"
                     /locus_tag="VKU03_13245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012562983.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA];
                     GO_process: GO:0034220 - monoatomic ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="large conductance mechanosensitive channel
                     protein MscL"
                     /protein_id="HLJ72279.1"
                     /translation="MLKEFRAFAMRGNVVDLAVAVVIGAAFGKIIDSLVNDIIMPIIG
                     IFGKADFSNYFIPLSSAVTAGNLVDAKKQGGVFAYGNFVTNVINFLIIAAALFLVIQV
                     INRLRKKEAEAPAAPPAPTTSEKLLTEIRDALVAK"
BASE COUNT          614 a         1233 c         1232 g          681 t
ORIGIN      
        1 gccgtcgccg atttttgcga tttccacatc ggcggctggc attggtatgt cttcaatatc
       61 gccgacacgg cgatcgtcgt cggcgtcgcg cttctcgcct atgacggcct tttcgccgaa
      121 aagcgcgtgc aggcgggcca ggccgacgcc agccggccgt gagcggccga gcaaagagtc
      181 cacaaacggc cgcaactgtg ataaaaaggt cgcatgaggg cagttttgtc ttgagggcgg
      241 cgctggcttt agaccagcgc caccgattag ccgaaatgct ttgaaagctg aggcgtcaat
      301 cgcacggtcg cgtccgcgga cgatgatcgt cggactttgg aaaggaactt ggggcgatga
      361 cgttcagggg ctcccatcgc agccttcttc tcgcagccgc cgtcgccgcg ggccttgccg
      421 cgcccgtggc cgctcgcgcc ggcgaggacg ggcagcagcc cttatgggaa gggctcggcg
      481 acatgatcgg cctgaccggc acggacaagg ttgacaagcc gatcaaatat ggcgagcggc
      541 cgaaactcgt cctgccgaag acgaccgatc tgccgccgcc cagccccgcc ctgtcgctga
      601 ccgccgattg gccgcacgat ccggacgtcg agaagatcaa gcgcgaaaag gaggcgatcc
      661 tccatcgcca ccagcgcagc ccgagcctcg acaaggcgag tctttatggc aggccgctgt
      721 cgcccgatct gctgcgctcg gatcacgccg cgcccggcac gctcggcgcc gccgaccatt
      781 gccagatcga tccgcgccac tgccactgga taaggcccga cattctcgaa aagctcggct
      841 tgaagaagga agaggacaca gtcgtcgcgg ggcaggagcc ggatcgcgat tggctgaccg
      901 atccgccgaa gggctatcgc gtgccggcgg ccaacaccaa ggcgacgttc gaggcggccg
      961 atcacgccga tcacggcgat cagcgctacg atctctacaa gccgccgtcg caatagcggc
     1021 gagccgggcg ccggcggacc tctccgtcct aacgatggcg ggtcctgcat tgctcccgca
     1081 cggcgcgcgg gcgatcatcc tcgccgaatc gccctaagcg ctggagctcc gaatgaagcg
     1141 gtttttcaag ccggcgcgga tcgcctggtc cgccgcggga caggcgcatg gcgtcgcgca
     1201 cggcccggtt ttcaagcgat tgctgatcgc cggccagccg ggcgtcgacg aaaccggcga
     1261 ggttccggcc gatctggaag cgcaaatcgg cgcggctttc gacaatctgc tgagcgtagt
     1321 cgaagccggg cagatggcgg ccgaggatat tgtccgcatc atcgcctatg tcgccgcgcc
     1381 gcgcgcctat gaaatgttcg agcgcgtgcg cgagcgcaag ctcggcggct tgacgccggc
     1441 cgccagctat gtcgagacga gcgggttcag cgatccgcgc tggcgcgtgc tgatcgaagg
     1501 cgaagccgtg cgcgatcaaa cggcgtgacg gcggaccggc gatgacgctc gaaaaggacg
     1561 acagcgaggc aggcccgcgc ggcgagttga ccgttcggct catcgccatg ccgggcgaca
     1621 ccaacgccaa tggcgatatt ttcggcggct gggtgctgtc gcaaatggac caggccggcg
     1681 gcatagccgg cgtcgagcgc gcgcaaggcc gggtcgtgac gatcgccgtc gaggcgatga
     1741 ccttcatccg gccggtgcgc gtcggcgacg tgctctgcgt ctataccgaa gtcgagcgcg
     1801 tcgggcggac ctcgatgcgg attcatatcg aggcttgggc gcagcgattt cgtacgcatt
     1861 tccgcgagaa ggtgaccgac gcggtcttca ccttcgtggc ggtggacgac gaaggacggc
     1921 cgcggccgat cccgagagaa gaaagccaaa gatgatggcg cgtcattgcg agcgaagcga
     1981 aacaatccat ccatgggccg cagcgctcta cgaatggatt gcgtcgtcgc ttcgctcttc
     2041 gcaatgacgg gcgtgaatgg cgggggcagg ggcggtgcag gatagtcttg aatggtcgcg
     2101 cagccggttt gaatccttcg acagcatgga ggggctggcg gcgagcgaag ccagcgcgcc
     2161 gccgcgcttc ctttcgcttt tgccgcgagg caagctggtc cacgcggtct atcccggctt
     2221 cctcgtcgcc gcgacgatcg cgctcgcttc gtcctggctc gcgcagcact atcgcgcgcc
     2281 ggtcatgctg ttcgcgctgc tcttcggcat ggcgtttcac ttcctgcacg aagaggggcg
     2341 ctgcgtcgcc ggcatcgaat tctcgtcccg tactgtgctg cgcctcggcg tcgcgctgct
     2401 cggcgcgcgc atcacgctcg cgcagatcgc gtctctcggc gttctgcctg tcgcgacggt
     2461 gatcgtcggc gtcgcgacga cgatatcgat cggcgtcctg ctggcgcgcc gactcggctt
     2521 gcgttcgagc ttcggggcgc tgtccggcgg gtcggtggcg atttgcggcg cttcggccgc
     2581 tttggccatc gcctcggtgc tgccgcggca cgaggacagc gagcgcgacg ccatcctgac
     2641 cgtcgtcgcg gtgaccgcgc tctcgaccct ggcgatgatt ctctatccga ttttcgtcgt
     2701 cgcgatcggg ctcgaccatg tccatgccgg catattcctc ggcggcacga tccacgacgt
     2761 cgcgcaagtg gtcggcgccg gctacaccgt ctcgcaggag accggcgaca tcgccaccta
     2821 tgtcaagctg ctgcgcgtcg ccatgctgct gccggccgtt ctcgcgatct ccttcgcgct
     2881 gacccgcgcc ggcgcggcgc gggccgcgac gccggctttg ccagggttcc tgatcggctt
     2941 tgcgcttctc gtcgtcgtca acagctcggg cttccttccg aaaatcgccg ccgatctgat
     3001 gagcgacgtt tcgcgctggt gcctcgtcgc agcgatcgcg gcgctcggca tgaagacctc
     3061 gttcaagaac cttttcgccg tcggctggcg gccgatcgcg ctgatgctct gcgagacgct
     3121 atggatcgcc ggcctcgtgc tcgccagcgt tctgctcgcg gcctagccgc tccatcgcgc
     3181 ttttgacagt agagtcggct cggctaagct tgaatgaatc ggcgccgcgc gccgattcta
     3241 agcgagaggg ggatctcatg cttaaggagt ttcgcgcctt cgccatgcgg ggcaatgtcg
     3301 tcgacttggc ggtcgcggtg gttatcggcg ccgccttcgg caaaattatc gattcgctgg
     3361 tcaacgacat catcatgccg atcatcggca tattcggcaa agccgatttc tccaactatt
     3421 tcatcccgct ctccagcgcc gtcaccgccg gaaacctggt cgacgccaag aagcagggcg
     3481 gcgtcttcgc ctatggcaat ttcgtgacca acgtcatcaa tttcctgatc atcgccgcgg
     3541 ctttgttcct cgtcatccag gtgatcaacc gcctccggaa gaaggaggcc gaagcgccgg
     3601 ccgctccgcc ggcgccgacc acaagcgaga agctgctgac ggaaatcagg gacgctctgg
     3661 tcgcgaagtg aggcgacgcg gcccttctct ccgcttcgcg cctacggcat gcccgcatct
     3721 cggcgcgggg ccatatcggc ccggcatcgt caagcggcca
//
LOCUS       DATGJW010000443         8431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_495445, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000443 DATGJW010000000
VERSION     DATGJW010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8431
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_495445"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VKU03_13250"
     CDS             complement(<1..207)
                     /locus_tag="VKU03_13250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72280.1"
                     /translation="MNRVASGGDIAKQERFGISSPNFLLSRLRKKQAQRSRLFRTLQA
                     RMTQISKRPAAIDAEARQGQAASRS"
     gene            complement(262..723)
                     /locus_tag="VKU03_13255"
     CDS             complement(262..723)
                     /locus_tag="VKU03_13255"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563490.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit chi"
                     /protein_id="HLJ72281.1"
                     /translation="MTDIRFYHLERRRLEDALPRLLEQALAEGARVVVEAPSSELIDA
                     LDERLWTYSDDSFLPHGQARDGEPEAQPILLTTAAENPNNAAWRILVGGARALPALSG
                     DAALERLIVLFDGADPDARAAARAQWTEIKASGFAPSYWREDDTGRWTQGR"
     gene            complement(857..1402)
                     /locus_tag="VKU03_13260"
     CDS             complement(857..1402)
                     /locus_tag="VKU03_13260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72282.1"
                     /translation="MGLFMKSGAALVLAALIAAPQGAAAKPPLAGCDAFIATLRKIAS
                     DLQVDFTHSIIVSRARSTANVFDVTTNAEVDGTLTCRGDEMERFEARVAEPASARTLT
                     NFERFETAALRAALGWDEARAQAKLREMSGDAREFLAASKQRGDAYIAGKTEEHLPGA
                     VGLGLIYTETDRALIVVGPGG"
     gene            complement(1413..2912)
                     /locus_tag="VKU03_13265"
     CDS             complement(1413..2912)
                     /locus_tag="VKU03_13265"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013165922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030145 - manganese ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HLJ72283.1"
                     /translation="MSVTVKVDFAPLAAPKGATFVVFVGADLKLAKRVAELAPAVADL
                     LAGAAATARFTGKAQTALDIFAPRGLDAPRLLVVGIGGAKEAKETDFVTLGGFVMGKL
                     GGGRDAAVAFVAPDGEWGAEAAADFLLGLRLRAYRFDKYKTKKKEDEENGGEAGEVTI
                     GLADPAAARRAARGREAVAEGVELARNLVNEPPNVLFPEEFAKRAAHLSKLGVEIDTL
                     DEKAMAKLGMGALLGVGQGSDKESRLVVMRWNGAKSAKAKPVAFIGKGVCFDTGGISI
                     KPAAGMEDMKGDMAGAACVVGLMHALARRKAKVNAIGAIGLVENMPGGDAIRPGDILK
                     SLSGQTIEVINTDAEGRLVLSDALWYVQDKYKPAFMIDLATLTGAILVALGQERAGLF
                     SNNDELADRLAAAGKATGEKVWRLPLDAAYDKLIDSKFADVKNTGGRHAGSITAAQFL
                     QRFVNNVPWAHLDIAGVGMGSQQNEINRSWASGWGVRLLDRLVADHYET"
     gene            3099..4223
                     /gene="lptF"
                     /locus_tag="VKU03_13270"
     CDS             3099..4223
                     /gene="lptF"
                     /locus_tag="VKU03_13270"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptF"
                     /protein_id="HLJ72284.1"
                     /translation="MTRIERYIFRTASLAFLAGLCALTAVVWITQALRQFDLLTSKGQ
                     TILVFLTMTGLTIPSLVAIIAPVALFVGVLYSLNKFNGDSELVVMSAAGISPGALLRP
                     FALLFALVFGLVAWMTLDVMPASFDAIQILTSRIRADFITNFARPGAFTELESGFVFH
                     YRERGPDGSLRGVFMQDRRDPAHITSYIAERGRTIEKNGSNYLVLDEGSYQRPAAGGD
                     SAIVTFKDYTIDLTQFAHRDETSKRPRERSTADLIAPDPKDETMRRLAGRFRAELIDR
                     LTAPFYAFVAGLIGFAALGEARTTRQGRGVAIAVAILAFAAVRLGGVAATSFVVSRPR
                     AEYIVWALPLIVCILCLDAIFHGPLIRLAVALPQRARAPR"
     gene            4220..5305
                     /gene="lptG"
                     /locus_tag="VKU03_13275"
     CDS             4220..5305
                     /gene="lptG"
                     /locus_tag="VKU03_13275"
                     /EC_number="7.5.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176126.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015221 - lipopolysaccharide transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015920 - lipopolysaccharide transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS export ABC transporter permease LptG"
                     /protein_id="HLJ72285.1"
                     /translation="MIGPTLARYMALRFAKTVLATFAGVFALIFTIDLVETLRRTGDS
                     PKATGVLMMMLSLFHTPTIAEQVLPFAVLFGAMIAFLNLSRRLELVVARAAGVSVWQF
                     LTPPILVVLLLGIASVTLYNPASAYLKQRADQIEAKIFGQAGQLSSSAGLWIRQKSVD
                     GQAIIRAEKASEGGVVLSGVTVFDFDRQGRFEERIDAANAALHHGFWRLADAKVVTPG
                     IETQSVRSYLLATNLTPREVSQSFTPPETVPFWSLPALAEQTERAGLDAAAYRLRYQE
                     LLARPLMLAAMVLIAASFSLRFFRMGGVGLMVSGGVAAGFVLYVCTKLVGDLGGAGFI
                     SAPVAGWSPAVVGCLCGVLALLHQEDG"
     gene            5305..7881
                     /locus_tag="VKU03_13280"
     CDS             5305..7881
                     /locus_tag="VKU03_13280"
                     /inference="COORDINATES: protein motif:HMM:NF016348.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LPS-assembly protein LptD"
                     /protein_id="HLJ72286.1"
                     /translation="MGRPARPPESVKGRRRSFALGGGALAFFLAGLILLTPACVSPSL
                     AQAANAAAKPGNPGAKKPDKMLVQANELVFDKDKNTVSAVGDAQIYYQGRILEADRVV
                     YDRAKKRVYAQGHAKFTDEHGDVTYGSRFELTQDFRDGFIDSVQTLTKDKARFSSPRV
                     ERSEGVVTVFEKGTYTACEPCQDHPERPPLWQVRSARIIHNNQTHTVYYEDSTLELAG
                     VPIAYIPYFSAPDPTVTRQSGVLAPHFIRDTRLGYGFGVPYFFDLAPNYDLTVDPTLL
                     SRQGLLGDVEWRHRLDNGAYTIRATGIFQQDPKAFLPAPYGSGGERFRGSVETAGKFY
                     INDRWAFGWNITALTDRYYFADYKLKTEDISHYYIADVVSSVYLRGQGDRGFFDLSGY
                     HFEGLTQNDFEKQEPFAAPVLDYNKTIDIPGADKGGIGGQATVDVNVASVSRVAALYQ
                     STGLQTLDKAFSLYNVCTTYTPGKCMVRGVAGNYSRASLQLSWQRKFIDPIGEVWTPF
                     IFARLDGEETALNTNQTFTFVNGAITDSISNSMQPAFFAGQQSDVSGRAMPGIGLEYR
                     YPWFSSTPIGSQVIEPIAQVIARPNEINPKLQPNEDAQSLIFDDTTLFEWNKYSGYDR
                     VEGGGRLNYGAQYTLNFNNGGHLNLVGGESIHLFGVNSFAVPDAANTGLESGLDKKYS
                     DFVARQTFAPISAVSFSTKEQFDSSTLAMKRFDFITNATYGPLTATLDFGRYAAQPEL
                     GYFYAREGLLTSAKYKFADHWSVNGTVVFDMSRHYYDVAGQSTPVLYAPTYGFGVGYS
                     DECTTVNLSYTSTLTDPIASTPQVRDQTVLLQITLRTLGELRASTSIGESAPETTTPV
                     VP"
     gene            7889..>8431
                     /locus_tag="VKU03_13285"
     CDS             7889..>8431
                     /locus_tag="VKU03_13285"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLJ72287.1"
                     /translation="MTRGAPLAMTMGDPAGIGPELILKAWLGRAAIGAPFFALADPRM
                     LAALAARLGLDVPIIAAEPAGAAEIFDAALPVVPLTREVLADSGRPDSKNAAAILESI
                     ERAVEFVRQGEASAVVTNPIAKSVLYQAGFQHPGHTEYLGELAARWGGAARPVMMLWS
                     PELAVIPVTIHIPLVEVPRAL"
BASE COUNT         1375 a         2861 c         2637 g         1558 t
ORIGIN      
        1 cgaccgcgaa gcggcttgac cttgacgcgc ctccgcgtcg atggcagcgg ggcgttttga
       61 gatttgcgtc atgcgcgcct gtaaggttcg aaaaagcctt gagcgctgcg cctgcttttt
      121 tctgagtctc gacaacagaa aattcggcga ggaaatcccg aaacgttctt gttttgcgat
      181 atcgccgcct gaagcgactc ggttcatgct cattttcccg gacggccctg cgcaaagcgg
      241 ggagaaggga tttcccccgg cctatcgtcc ttgcgtccag cgtcccgtat cgtcctcgcg
      301 ccaataggac ggagcgaagc cggaggcttt gatctcggtc cattgcgcgc gcgcggcggc
      361 gcgcgcgtcc ggatcggcgc cgtcgaacag aacgatcagc cgctccagcg cggcgtcgcc
      421 gctcaacgcc ggcagggccc gcgcgccgcc gaccaggatg cgccaagcgg cgttgttggg
      481 attctcggcg gcggtggtca gcaaaatcgg ctgggcttcc ggctcgccgt cgcgcgcctg
      541 gccatgcggc aaaaaactat cgtcggaata agtccagagc cgctcgtcca gcgcgtcgat
      601 caattcgctc gacggcgcct cgacgacaac gcgcgcgcct tccgcgagcg cctgctcgag
      661 aaggcgcggc aaggcgtctt cgagccttcg cctttcgaga tgatagaagc ggatgtcggt
      721 catggcgccg acatgccgtt ggcggcgccg tcattgcgag caaagcgcag caatccattc
      781 ataggacgcc gccctttgaa tggattgctt cgtcgcttcg ctcctcgcaa tgtcggcgtg
      841 gcgtacctga cacggctcag ccgcccggtc cgacgacgat cagcgcgcga tcggtctcgg
      901 tatagatgag cccgaggccg accgcgccgg gcaggtgctc ctcggtcttg ccggcgatgt
      961 aggcgtcgcc gcgctgcttg gaggcggcga gaaattcgcg cgcgtcgccg ctcatctcgc
     1021 gcagtttcgc ctgcgccctc gcttcgtccc agccgagcgc ggcgcgcaag gccgccgtct
     1081 cgaagcgctc gaaattggtg agcgtgcgcg cgctcgccgg ctcggcgacg cgcgcctcga
     1141 accgctccat ctcgtcgccg cggcaggtca atgtgccgtc gacctcggcg ttggtcgtca
     1201 cgtcgaagac gttggccgtg ctgcgggcgc gcgagacgat aattgaatgc gtaaagtcga
     1261 cctgcaggtc ggaggcgatc ttgcgcaacg tggcgatgaa ggcgtcgcag ccggcgagcg
     1321 gcggcttggc cgccgcgcct tgcggcgcgg cgatgagcgc ggccagaacg agcgccgcgc
     1381 cgcttttcat gaaaagcccc atggccccct tctcacgtct cgtaatgatc ggcgacgagc
     1441 cgatcgagca ggcgcacgcc ccagcccgac gcccaggagc ggttgatctc gttttgctgc
     1501 gagcccatgc cgacgccggc aatatcgaga tgcgcccaag gaacattgtt gacgaagcgc
     1561 tggagaaact gcgccgcggt gatcgagccc gcgtgccggc cgccggtatt cttcacgtcg
     1621 gcgaatttcg aatcgatcag cttatcatag gcggcgtcaa ggggcaggcg ccagactttc
     1681 tccccggtcg ccttgccggc cgcggcaagg cggtcggcca attcgtcatt gttggagaaa
     1741 agcccggccc gctcctggcc gagcgcgacg agaatggcgc cggtcaaggt ggccaggtcg
     1801 atcatgaatg cgggcttgta cttatcctgc acgtaccaaa gcgcgtcgga aaggacgagc
     1861 cggccttccg catcggtatt gatcacctcg atcgtctggc cggacaggga cttcaggatg
     1921 tcgcccggcc ggatggcgtc gccgcccggc atgttctcga caaggccgat cgcgccgatc
     1981 gcgttgacct tggccttgcg ccgcgccaag gcgtgcatca gcccgacgac gcaagccgcg
     2041 ccggccatgt cgcctttcat gtcctccatg ccggcggcgg gcttgatcga aatgccgccg
     2101 gtgtcgaagc agacgccctt gccgataaag gcgaccggct tggccttggc gctcttggcg
     2161 ccgttccagc gcatgacgac gagccggctc tctttgtccg agccctggcc gacgccgagc
     2221 aaagcgccca tgccgagctt ggccatcgcc ttctcgtcga gcgtgtcgat ctcgaccccc
     2281 agcttggata aatgcgcggc tcgcttggcg aattcctcgg gaaagagcac gttgggcggc
     2341 tcgttgacga ggttgcgggc gagttcgacg ccttcggcga ccgcctcgcg gccgcgggcc
     2401 gcccggcgcg ccgccgccgg atcggcgagg ccgatcgtca cctcgccggc ttcgccgcca
     2461 ttctcctcgt cttccttttt cttggtcttg tatttatcga agcgataggc gcgcaggcgc
     2521 aggccgagca gaaaatcggc ggcggcctcg gcgccccatt cgccatccgg ggcgacgaaa
     2581 gcgaccgcgg cgtcgcgccc gccgccgagc ttgcccatga cgaagccgcc gagggtgacg
     2641 aaatccgttt ccttcgcctc tttcgcgccg ccgattccga cgaccagcag gcgcggcgca
     2701 tcgaggccgc gcggcgcgaa aatatcgagc gcggtctggg ctttgccggt aaagcgcgcc
     2761 gtcgcggcgg cgccggcgag gagatcggcc acagccggcg caagctcggc gacgcgcttg
     2821 gccagcttca gatcggcgcc gacgaagacg acaaaggtcg ctcccttcgg cgccgcaagc
     2881 ggcgcgaagt cgactttcac ggtcacgctc atcgattcct ctaaagcggc cttgccggcc
     2941 ggaccgccat ctgcgccaga gcgccgcgac aaatcaagag ccgggcgggc cgccccgaaa
     3001 aggccccaaa gccgaggcga cggttttccg ggggccgccg catcggcgcg gcccggcgct
     3061 cttgcgcgaa gcgcggcaga atcggaaaag cggacccaat gacgcgcatc gagcgctata
     3121 ttttccgcac cgcctcgctg gccttcctcg ccggcctgtg cgcgctgacg gccgtggtct
     3181 ggatcaccca ggctttgcgg caattcgatc ttttgaccag caagggccag acaatcctcg
     3241 tctttctgac gatgaccggc ctgaccattc cctcgctcgt cgccatcatc gcgccggtcg
     3301 cgctgtttgt cggggtgctc tacagcctca acaagttcaa cggcgacagc gagctcgtgg
     3361 tgatgagcgc ggccggaatc tcgcccggcg cgctgttgcg gcccttcgcc ttgctcttcg
     3421 ccctggtctt cggcctcgtc gcgtggatga cgctcgacgt gatgccggcg agcttcgacg
     3481 ccatccagat cctcacttcg cgcataaggg ccgacttcat caccaatttc gcccggccgg
     3541 gggcgttcac cgagctggaa tcgggcttcg tcttccatta tcgcgagcgc ggcccggacg
     3601 gctccctgcg cggcgttttc atgcaggacc gtcgcgatcc ggcccatatc accagctaca
     3661 tcgccgagcg cggcaggacg atcgaaaaga acggctcgaa ttatctcgtg ctcgacgagg
     3721 gaagctacca gcggccggcc gccggcggcg attcggcgat tgtcaccttc aaggactaca
     3781 cgatcgatct cactcaattc gcgcaccggg acgagacgtc gaaacggccg cgcgagcgct
     3841 cgaccgccga cctcatcgcg ccggatccca aggacgagac gatgaggcgg ctcgccggcc
     3901 ggtttcgcgc cgaattgatc gaccggctga ccgcgccgtt ttacgccttc gtcgccggcc
     3961 tgatcggctt cgccgcgctt ggcgaagcgc gcaccacccg ccagggccgc ggcgtcgcga
     4021 tcgcggttgc gatcctcgcc ttcgccgctg tgcggctcgg cggcgtcgcc gcgaccagct
     4081 tcgtcgtctc ccgcccccgt gccgaatata tcgtctgggc tcttccctta atcgtttgca
     4141 ttctgtgtct tgacgcgatt ttccacggcc cgctgatccg cctcgccgtc gcgctgccgc
     4201 agcgggcccg ggcgccgcga tgatcggtcc gacgctcgcg cgctacatgg cgctgcgttt
     4261 cgccaaaact gtccttgcga catttgccgg cgtgttcgcg ctcatcttca ccatcgacct
     4321 cgtcgagacc ttgcggcgca ccggcgactc gccgaaagcg accggcgtcc tgatgatgat
     4381 gctgtcgctt tttcatacgc cgaccatcgc cgagcaggtc ctgcctttcg cggtgctgtt
     4441 cggcgcgatg atcgccttcc tcaacctgtc gcggcggctt gaactggtgg tggcgcgcgc
     4501 cgcaggcgtt tccgtctggc aatttctgac cccgccgatc ctcgtcgtgc tgctgctcgg
     4561 aatcgcctcg gtgacgctct acaatcccgc ctcggcctat ctcaagcagc gcgccgacca
     4621 gatcgaggcc aagatttttg gccaggccgg acaattgagc agctcggccg gcctgtggat
     4681 ccgacagaaa agcgtcgacg gccaggcgat catccgcgcc gagaaggcga gcgagggcgg
     4741 cgtcgtcctt tccggcgtca cggtcttcga tttcgaccgg caagggcgct tcgaggagcg
     4801 catcgacgcc gccaacgccg cgctgcatca tggcttctgg cggctcgccg acgccaaggt
     4861 cgtcacgccg gggatcgaga cccaatcggt ccggtcttac ctgctcgcga ccaacctgac
     4921 gccgcgcgag gtctcgcaat ccttcacgcc gccggaaacc gtcccatttt ggagcctgcc
     4981 ggccctggcc gagcagaccg agcgcgccgg cctcgatgcg gcggcgtatc ggctgcgata
     5041 tcaagagctt ctggcgaggc cgctgatgct ggcggcgatg gttttgatcg ccgcttcctt
     5101 ttcattaaga tttttccgaa tgggcggcgt cggcctcatg gtttccggtg gcgtggcggc
     5161 gggcttcgtg ctttatgtct gcaccaaatt ggtcggcgat ctcggcggcg ctgggttcat
     5221 cagcgcgccg gtcgccggct ggtcgccggc ggtggtcggg tgcttgtgcg gggtcttggc
     5281 gcttttgcat caggaggatg gttgatgggc cggcccgccc gccctcctga atccgtcaag
     5341 ggccgccggc gcagctttgc gctgggcggc ggagctttgg ccttctttct cgccggattg
     5401 atcctgctga cgccggcttg cgtgtcgccg tccctggcgc aggcggcgaa cgccgccgct
     5461 aagccaggca atcccggcgc gaagaagccg gacaagatgc tcgtccaggc caacgagctg
     5521 gtcttcgaca aggataagaa caccgtctcg gcagtcggcg acgcgcaaat ctattatcag
     5581 ggccgcatcc tcgaagccga ccgcgtcgtc tatgaccgcg ccaagaagcg cgtctatgcc
     5641 caaggccacg ccaaattcac cgacgagcat ggcgacgtga cctatggctc gcgtttcgag
     5701 cttacccagg atttccgcga cggcttcatc gacagcgtcc agacgctgac caaggacaag
     5761 gcgcgctttt cctcgccgcg ggtcgagcgc tcggaaggcg tcgtcaccgt cttcgaaaag
     5821 gggacctaca cggcttgcga gccctgccag gaccacccgg agcgcccgcc gctctggcag
     5881 gtccgctcgg cgcggatcat ccacaacaat cagacgcaca ccgtctatta cgaggattcc
     5941 acgctggagc tggccggcgt tccgatcgcc tatatcccct atttctcggc gccggacccg
     6001 accgtcacgc ggcagagcgg cgttctcgcg ccgcatttca tccgcgacac gcggctcggc
     6061 tatggcttcg gcgttcctta tttcttcgat ctggccccca actacgatct gactgtcgat
     6121 ccgaccctgc tcagccggca aggcctgctc ggcgatgtcg aatggcgcca ccgcctcgac
     6181 aacggcgcct acacgatccg cgccacgggc attttccagc aggacccgaa ggccttcctg
     6241 cccgcgcctt acggctcggg cggcgagcgg ttccgcggtt ctgtcgagac cgccggcaag
     6301 ttctatatca atgaccgatg ggcctttggc tggaacatca cggcgctgac cgaccggtat
     6361 tatttcgccg actacaagct caagaccgag gacatttcgc attactacat cgccgacgtg
     6421 gtctcgtcgg tctatttgcg cggccaaggc gaccgcggct ttttcgatct cagcggctat
     6481 catttcgaag gcctgacgca gaacgacttc gaaaagcagg agccgtttgc ggcgccggtc
     6541 ctcgactaca acaagacgat cgacattccc ggcgccgata agggcggcat cggcggtcag
     6601 gcgacggtcg atgtcaacgt cgcctcggtc tcgcgcgtgg cggcgctcta ccaatcgacc
     6661 ggcctgcaga cgctcgacaa ggctttcagc ctctacaatg tctgcacgac ctatacgccc
     6721 ggcaaatgca tggtgagagg cgtcgccggc aattacagcc gcgcttcgct gcaactttcc
     6781 tggcagcgca agttcatcga tccgatcggc gaggtctgga cgccgttcat cttcgcccgg
     6841 ctcgacggcg aggaaacggc gctcaacacc aaccagacct tcaccttcgt caatggcgcg
     6901 atcaccgatt cgatcagcaa ttcgatgcag ccggcctttt tcgccggcca gcagagcgac
     6961 gtttccggca gggcgatgcc gggaatcggc ctcgaatacc gctatccgtg gttctcctcg
     7021 acgccgatcg gctcgcaggt gatcgagccg atcgcccagg tcatcgcccg gccgaacgaa
     7081 atcaacccga agctgcagcc gaacgaggat gcgcaaagcc tcatcttcga cgacacgacc
     7141 ctgttcgagt ggaacaaata ttcaggctat gaccgggtcg agggcggcgg acggcttaac
     7201 tacggcgcgc aatatacgct gaacttcaac aatggcggcc atctcaacct ggtcggcggc
     7261 gagtcgatcc atctcttcgg cgtcaattcc ttcgccgttc ccgacgccgc caataccgga
     7321 ttggaatccg gcctcgacaa gaaatattcc gacttcgtcg cgcgccagac cttcgcgccg
     7381 atctcggcgg tctccttctc gaccaaggag caattcgaca gctcgacgct ggcgatgaaa
     7441 cgcttcgatt tcatcaccaa cgcgacctat ggaccgctga ccgcgacgct cgatttcggc
     7501 cgctacgccg ctcagcccga gctcggctat ttctatgcgc gcgaaggcct gctcaccagc
     7561 gccaaataca agttcgccga ccattggtcg gtgaacggca cggtcgtgtt cgacatgtcg
     7621 cgccactatt acgacgtggc cggacagagc acgccggtcc tctatgcgcc gacctatgga
     7681 ttcggggtcg gctatagcga cgaatgcacc accgtcaatt tgagctacac gtcgacgctg
     7741 accgacccga tcgcgtcgac gccgcaggtt cgcgaccaga ccgtactcct gcagatcacg
     7801 ctgcggactt tgggcgagtt gcgcgccagc acgagcatcg gcgaatcggc gccggagacg
     7861 acgacgccgg tcgtgccgtg agcgcgccgt gacgcgtggc gcgccgctcg cgatgacgat
     7921 gggcgacccg gccggaatcg gccccgagct cattctgaaa gcctggctcg gtcgagccgc
     7981 gattggggcg ccgtttttcg cgcttgccga tccgcggatg ctggcggcgc ttgccgcgcg
     8041 gctcggtctc gatgttccga ttatcgccgc cgagcctgcc ggcgcggcgg aaattttcga
     8101 cgccgcgctg cccgtcgttc cgctgacgcg tgaagtcttg gccgattccg gccggccgga
     8161 ttcgaaaaac gccgccgcga ttctcgaatc gatcgagcgc gccgtcgaat tcgttcgcca
     8221 aggcgaagcg agcgctgtcg tcaccaatcc gatcgccaag agcgtgctct atcaggcggg
     8281 ctttcagcat cccggccata ccgaatatct cggcgaattg gcggcgcgct ggggcggcgc
     8341 ggcgcggccg gtcatgatgc tgtggtcgcc cgaactcgcc gtcattccgg tcacgatcca
     8401 tatcccgctc gtcgaggttc cgcgcgcgct g
//
LOCUS       DATGJW010000444        12729 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_190448, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000444 DATGJW010000000
VERSION     DATGJW010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12729)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12729)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12729
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_190448"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VKU03_13290"
     CDS             complement(<1..344)
                     /locus_tag="VKU03_13290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72288.1"
                     /translation="MSRHEIASDPMRATLRESRMVVERLCQSIGVPDGVLRSVTDCGV
                     YSAALGLSGYPGFERQLALLQRDAAGQMSARQERERIFFDGGGRHAWFAAEPALDLLV
                     SVYRIAGAAEMVA"
     gene            complement(341..2074)
                     /locus_tag="VKU03_13295"
     CDS             complement(341..2074)
                     /locus_tag="VKU03_13295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762471.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72289.1"
                     /translation="MPSAAERPTNYHPPGPRLRDPALLMRPERLAALQPSRVSASRAL
                     IARAMKGRWKITRTAFDLDERARGAACYRIEADGWEFSFPVFSFEPSGQLRTFRIIGR
                     SWDMMGALVEGHISKTDMDLTCQEMPKLYHGRATPGTLVWCRANRSARVLDYCVESLA
                     GGAQPRTDIMAAASYAMRNTGLDGNGTFGTRSFAALERGHPLRASLAAQMLAAYMMRV
                     FAYDLIEHLSRARGGARTVAMNSDLARFLGIGNGSALGLMFFVHNHPRLLDRWLSIRE
                     QAIAAAKALEIGRDAAPLDLALRLIDRAIVYRRQDKTEYDRLTSSAAIADDLQRIRTE
                     LGRLRDRLRAGAASTPTPLVEFCDSIEGRFNADAIETLNSLLIELVPDLADRLVQTLI
                     VDEELVGRPEMPVARLRDIVRAEYRWAFDLDLTSERSRRYIWYKSVNAEEPRRGPREE
                     APEALSLGLDLPRLIVALDRDLAEADGKSSVARFLIAHPEHRLIATRVQALAGLPYHS
                     PHADIMSEDFMPAHITRMLNGAVHGLDKTRDAMGRVIRGVIMQGAPLPEELAAGAGDP
                     HWFNPPEPRVS"
     gene            2242..2814
                     /locus_tag="VKU03_13300"
     CDS             2242..2814
                     /locus_tag="VKU03_13300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72290.1"
                     /translation="MIKDRRGDCAHHNVLGGNSDAGSPRLSEHLVFRKLGAALGHPTN
                     FFWVGKSHQDSSGRRVLQRHRRADPGAGLKDSLGLDPLDDDRRAVLDDGQRRRFVRLR
                     DEFAQNWEGPVAEASLGESCAGDFHEPHRQAEATIFDLLDPARFHERDEALINRRASP
                     AEIGGGLGEPHWTIPRSKIIDEGKSLERRA"
     gene            complement(3336..3584)
                     /locus_tag="VKU03_13305"
     CDS             complement(3336..3584)
                     /locus_tag="VKU03_13305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72291.1"
                     /translation="MRVSLGIAMIFLASSIAAAEPPLSIERLLSEGWEIAGYASGYDN
                     RTSLILFRHAGTNALVQCGVLYDVTRNPRTIINCYELR"
     gene            complement(3751..5025)
                     /locus_tag="VKU03_13310"
     CDS             complement(3751..5025)
                     /locus_tag="VKU03_13310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018270966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ72292.1"
                     /translation="MAERIRRFMAERIYPNERRFYQEAERLGPWAVYPVVEELKALAR
                     AAGLWNLFLPAAAGGLSNLDYAPLCEIMGRSHLAPEVFNCSAPDTGNMETILRYGTEA
                     QKETWLKPLLAGEIRSAFAMTEPDVASSDAANVASSIRREGDHYVINGRKWYTTGATD
                     PRCKIIIFMGQTDPDNPNRHRRQSMILVPKDAPGVRVVRALPVLGFYGVPDRASEVTF
                     SDVRVPAENILLGEGRGFEIAQGRLGPGRIHHCMRLIGLCERTLERMCRRAESRVAFG
                     RPLAEQTVTLERIAEARILIEQARLLTLKAAHMMDTVGAKAARQEIAMIKVVAPNVAQ
                     RIVDWAIQLFGGGGTSNDHFLAASLATARLLRLADGPDEVHRNQIGYFELLRHRNADP
                     AQTGGAAAPLAPAEAEAWAAGGPWPRPPERRD"
     gene            complement(5395..6177)
                     /locus_tag="VKU03_13315"
     CDS             complement(5395..6177)
                     /locus_tag="VKU03_13315"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72293.1"
                     /translation="MDRVKGKVALITGAGTGVGRACMKLFAQEGAKVVGVSRTQSNLD
                     ETLAQVKAAGGDGAVVAADLSTEAGAKKAFDAALSAYGRVDILVNSAGVGYSWLEKSP
                     GSMGPIDDTTPEKWAEVMAINLDSLFHMCRLAIPQMKKQGGGAIVNVTSISGFQGLPV
                     AHTYTAAKGAAINLTRSLAITYCGDNIRTNCIAPGFIKTPMVESVLGLFDDPAMADRL
                     CPMKRPGTAEEMAYACLYLGSDEASYCQGTVLVVDGGTTARQ"
     gene            6315..7367
                     /locus_tag="VKU03_13320"
     CDS             6315..7367
                     /locus_tag="VKU03_13320"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HLJ72294.1"
                     /translation="MRAAVFHELGKPLQIETVAKPQAGPGEIVLKVHYCGVCGSDLHA
                     THPGVFVVPDGTILGHEFAGEIVESGAPGWKVGDVATALPNNACEECRKLGLGECKDN
                     LGIMCPRNTLTGFHPSAPGAYAEYVKFSAKEALRLPSAVKSREGAAVEPLAVGLHAVN
                     RGKVTVGERVLILGGGPIGLATAVFSKLAGARDVVVSEFAPARREAAGAMGATAVIDP
                     SKEEVGEAFARKAGAPPDVIFECVGVPGMIQKAIDLSRTYGRIVVVGVCMVEDTTTPI
                     SAIFKETNIQYVLGYGRPDWRLVLDLLDSGRVDPRPMITDTVALEELPAAFEALRKPT
                     TQIKVMVRPGERSGSL"
     gene            7466..8209
                     /locus_tag="VKU03_13325"
     CDS             7466..8209
                     /locus_tag="VKU03_13325"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ72295.1"
                     /translation="MDRDVAAPAVKPRASGRSAAEAAERAKMTSPDRVVDAIIRSIRA
                     GTYVPGQRLIEADLTRDYRVSRGPVREALKRLAAEGVLTLTRHRGAYVRALSRIEVRD
                     SLMVLEALVGLMANLAARRIEEDDNAARMREAYQRLLAFKHDGGPAAFLDERRAFYDT
                     ILQIGGNRELKRMLPLMQIHLLRMQFQAYITPRDREKQFKEYETITETILAGDSVRAQ
                     KVAALHVRRTRLSLMRLPDEAFPAIRSAD"
     gene            8214..8954
                     /locus_tag="VKU03_13330"
     CDS             8214..8954
                     /locus_tag="VKU03_13330"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72296.1"
                     /translation="MTILIAGGTKGIGLAVAKAFAPEAGDVFLGYHSDDEAAQEAAAS
                     VEAAGGRPHLVKADVAAPEGCAAMAEAVRTSAGRLDQLVHCAVDAYASSALGADPARF
                     ARAISANGASLLYLVQAALPLMPRGASIFYLTSRGGRIVVPNYAAVGVAKALAESLMR
                     YLAVELAPKGVRINAVAPAIVETDAVRALFGGEAANLVRAAAEHNPSGRGVTDADYTN
                     LMRWLASPEATFIQGQVIFVNGGANLSA"
     gene            complement(8958..10151)
                     /locus_tag="VKU03_13335"
     CDS             complement(8958..10151)
                     /locus_tag="VKU03_13335"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018427953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HLJ72297.1"
                     /translation="MRRAVIVDVVRSPFGRGRPGGALSGLHPVDLYASVLAALVRRTG
                     VDPALIEDVITGCVLQVAEQAGNIGRQAALAAGLPESTPAVTLDRKCGSAQQAFDFAA
                     QGVVAGAYDVVIAGGVEMMSLVPMRLNRMGKDNEGPAFHRRYPQGLVRQGVSAELIAA
                     RWGIEREAMDRFALESHRRAIAAEEAGLTRRAIVPIETQAADGAAGIVETDEGPRRDT
                     SIEKLAALKPAFEDAAMAARFPQIRWSVTAGNSSQVTDGAAAVLIAEESVARKLGLKP
                     RAAITHFALAGDDPILMLTAIVPATRKLLRRAGLSVDRIDAFEVNEAFASVVLAWLKE
                     TGADPERVNAYGGAIALGHPVGASGGRLLANLVARLEETGGRTGVQTMCESGGMANAT
                     LIERL"
     gene            complement(10190..11653)
                     /locus_tag="VKU03_13340"
     CDS             complement(10190..11653)
                     /locus_tag="VKU03_13340"
                     /inference="COORDINATES: protein motif:HMM:NF012885.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72298.1"
                     /translation="MAHIELLDETMRDGQQSLWGMRMQAGMALPVAPLIDRCGFRVID
                     LAGSSLFEVMIRTCRENPWEGLDLLVGAMPRTRIRGGMRSNASVTFGVTPDSLMDAWM
                     RQLNRHGCRSFWIYDVLFNIDKMRRLAKVAKEFGAEVAGSIMFALSPVHTDEYYADKA
                     DKLSASPDIDTLLLYDTAGVLDKERLKTLVPAIKAKARGKPIEFHANNILGQSAKAYL
                     DAIDLGVAILHTASRPMANGPSVPSTEIMLHNLELLGHTHGVDKALLPAIAEHFEKVG
                     KAAGFPVNQHNEYDVLSIRHQIPGGMTGTLKAQLAQHNMTDKLDEVLRETAEVRRDLG
                     YPGMATPFSQLVGIQAVLNVVTGKRYSIIPDEVIQYAAGFYGQTVAPIDPNVLDMIMA
                     APRAKQVAGSPPEQPTIEELRRRYGVDDDDELILRALVPESDLEKMRAAGPVRRDYPL
                     LSSPELDQVQKLMRAAKSPVVQIRTARLSLDLQRQTG"
     gene            complement(11657..>12729)
                     /locus_tag="VKU03_13345"
     CDS             complement(11657..>12729)
                     /locus_tag="VKU03_13345"
                     /inference="COORDINATES: protein motif:HMM:NF014805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72299.1"
                     /translation="GIAFVGPSSDSIEAVGDKLAARRLATAAGVATTPGSGQLATEEE
                     AVAAANRIGFPALIKASAGGGGRGMVIARDATSLRDGFARASAEAKEAFGDGTLFLER
                     YVERARHVEVQVMGFGDGRVLHFGERDCSMQRRYQKMIEEAPAAALPAATRSRLHEAA
                     VALLASIGYRNAGTVEFLYDVERDDFYFMEVNARIQVEHPVSEMICGVDLIRLQLEIA
                     GGAPPPIAQGDIVQHGHAIEARILAENPQRGFLPSPGRIVRWRPPQGEGVRLDTAIEE
                     GSVVSPYYDSMIAKLIVHAPDRPRAVARLREALDSFEVEGVATNIALLRAIAGHADFI
                     GARLDTRWLENVFLPHYGQPEG"
BASE COUNT         2135 a         4221 c         4213 g         2160 t
ORIGIN      
        1 gcgaccattt ccgctgcgcc ggcgatacga tatactgaga ccaatagatc gagagcgggc
       61 tcggccgcga accaggcgtg ccgaccgccg ccgtcgaaga aaattctttc ccgctcctgc
      121 ctagcgctca tctggccggc ggcgtctcgc tgcaacaagg ccaactggcg ttcgaagcct
      181 ggatagcccg aaagaccgag cgcggccgaa tagaccccgc aatccgttac ggagcgcagc
      241 acgccgtcag gcacgccgat cgactggcaa agccgctcca ccaccatgcg gctttcacgt
      301 aaagtcgcac gcatcggatc agaagcaatt tcgtggcggc tcatgagacg cgaggctccg
      361 gcggattgaa ccaatgggga tcgcctgcgc cggcggccag ctcttcggga agcggcgccc
      421 cttgcatgat cacgccgcgg atcacccggc ccattgcgtc gcgagtctta tcaaggccat
      481 gcacggcccc gtttagcatc cgggtgatgt gagcgggcat gaagtcctcg ctcatgatat
      541 cagcgtgagg cgagtgataa ggaaggccgg ccaaagcctg tacgcgggtt gcgataagcc
      601 gatgttcggg atgggcgatg aggaaccggg cgacgctgga tttgccgtcg gcctcggcca
      661 ggtcgcgatc aagcgccacg atcagccgcg gcagatcaag cccgaggctt agtgcttccg
      721 gcgcctcttc acgcggaccg cgtcgcggct cttccgcgtt gacggatttg taccagatgt
      781 agcggcgcga tctttccgag gtcagatcga gatcgaacgc ccagcgatac tcggcgcgga
      841 caatgtcgcg aagtcgggcg accggcattt ccggccggcc gaccagctct tcatcgacaa
      901 tcagcgtctg gacgagccga tccgccagat cagggacgag ttcgatcaga agcgagttca
      961 gcgtctcgat cgcgtcggcg ttgaagcggc cctcgatcga atcgcagaac tcgaccaggg
     1021 gtgtcggcgt tgaggccgcg ccggcgcgca ggcggtcgcg aaggcgcccg agctccgtgc
     1081 ggatgcgctg aaggtcgtcg gcgatggccg cgctcgatgt caaacggtca tattcggtct
     1141 tgtcctgccg gcgatagacg atcgctcgat cgatcaaccg aagcgcaagg tcgagcggcg
     1201 cggcgtccct gccgatttcg agcgccttgg cggcggcaat cgcctgctcg cgaattgaca
     1261 gccagcgatc cagtaggcgc gggtgattgt gcacgaagaa catcaaaccc aatgccgacc
     1321 cgtttccaat gccgaggaaa cgcgccaaat cggaattcat tgcgactgtc cgagctccgc
     1381 ctcgcgcgcg cgacaggtgc tcgatgagat cgtaagcgaa aactctcatc atataagccg
     1441 ccagcatttg cgcggcgagc gaggcgcgca gcggatgacc ccgttcgagc gcagcgaagc
     1501 tgcgcgtgcc gaatgtgcca ttgccgtcga gcccggtatt gcgcatcgcg tagctcgccg
     1561 ccgccatgat atccgttcgc ggctgagctc cgccggcgag cgattcgacg caataatcga
     1621 gaacgcgggc gctgcgattc gcccggcacc agaccagcgt ccccggcgtt gcacggccat
     1681 ggtagagctt cggcatttcc tgacatgtga ggtccatgtc cgttttggaa atgtgacctt
     1741 caaccaaagc gcccatcatg tcccacgatc ggccgatgat ccgaaaggta cgcaattgtc
     1801 cggacggttc gaaggaaaag accggaaagg agaattccca accgtcggcc tcgattcgat
     1861 agcaagccgc gccgcgcgcg cgctcgtcaa gatcgaaagc cgtcctcgtg atcttccagc
     1921 ggcccttcat ggcgcgggcg atcagcgcgc gcgacgcgct cacccgcgac ggctgcagcg
     1981 cagcaaggcg ctcagggcgc atgagcaatg cggggtcgcg cagacgtggg cccggcgggt
     2041 gataattggt cggtctttcc gcggcgctcg gcatcaagac tccgttccgg tcgattgagc
     2101 gacagcgccg ccgctccacg ccttgccgcc aagcatgatc gagagcctgc cggctgcggc
     2161 gatcaaaggc gctttcacct tctcgacgtt caagcggccc ttcggtccga cgagcgagag
     2221 ggcggcgacg cagcgctggc catgatcaaa gaccggcgcg gcgattgcgc tcaccacaac
     2281 gtgctcggcg gcaatagcga cgccggttcg ccgcgtctct cggagcacct cgtcttccga
     2341 aagctcggcg ccgctcttgg ccatccgacc aatttcttct gggtcggcaa aagccaccag
     2401 gattcgtccg gacgccgagt ccttcaacga catcgccgcg ccgatccggg cgcgggcctg
     2461 aaggattccc tcggcctgga cccgctggat gatgaccgcc gcgccgttct ggacgacggc
     2521 caacgacgcc gtttcgtgcg tctccgcgac gagttcgcgc aaaattggga gggtcctgtc
     2581 gccgaggcct ccttgggaga gagctgcgcc ggcgattttc atgagccgca tcgtcaggcg
     2641 gaagcgacca tcttcgattt gctcgaccca gcccgcttcc atgagcgtga cgaagcgttg
     2701 ataaatcgtc gagcgtcccc cgccgagatc ggcggcggcc tcggcgagcc gcactggacg
     2761 atcccgcgtt ccaagataat cgacgagggc aaaagtcttg agcgccgtgc ttaagggccg
     2821 cacggcggtc gttgacgatg ccgaagcgcc aatttgtatt ctattagaat actctgtctg
     2881 catataaaat atagttgttc cggagtatcg gcgttgtcaa gcccgttcgg catgcctcaa
     2941 agcccgggcg gctgccgctt gttcgaggga ggcgaacgcc tcgagcttgt ggcgctgctt
     3001 tatcgacgcc ggcatctcca gataggttga gcgcgtttgc gatcgcgcca gaccttcttg
     3061 gccgaccgag tttgccgggg tcgaggaggc atgtccccga acggctcttt ggcgagcgtc
     3121 tgcgttcggc cttgtccgat gcgtggcgac gcgtcgtctt tgtaccggat tagatccccc
     3181 tcgggggggg gaaggtgcgg ctacttagcc gatcaatctc acgtcggtcg ggttggccca
     3241 gcctgtccgc gccacgctgc cggcgcggcg ggagtcacgg cgcgctgaag acgtaaacgc
     3301 catctccgaa accttgttgc ggtcgaccgt tccgctcatc gcagctcgta gcaattgatg
     3361 atcgtgcgtg gatttctcgt cacgtcatag aggacgccgc actgaaccag cgcgttcgtt
     3421 cctgcatgac ggaagaggat aagcgacgtt cggttgtcgt aaccgctcgc atatccagcg
     3481 atctcccagc cctcggaaag gagtcgctcg atcgatagcg gcggctcggc ggccgcgatc
     3541 gacgaggcga ggaaaatcat cgcgatgcct aggctgacgc gcatgtgagg acctccaagc
     3601 gcgcggttga atcttttcgt cgcatcgttg cttcgcccac gtggcgcgcc acgcttcaac
     3661 agactttcga aaagtaccag agaatatcgg tcaatcgaag gcgagcggtt cacgcggcgc
     3721 gccgatctgg gagcggcatg ggctgtcgtc tcaatctcgc cgctctggcg gcctcggcca
     3781 tggccccccg gccgcccaag cctcggcttc tgcaggagcg agcggggcgg cggcgccgcc
     3841 ggtttgcgcc ggatcggcgt tccgatggcg cagcagctcg aaatagccga tctgattgcg
     3901 atggacttcg tccggtccgt cggcgagccg cagcaaccgc gctgtggcga gcgaggcggc
     3961 gaggaaatgg tcattgctcg tgccgccgcc gccgaaaagc tggatggccc agtcgacgat
     4021 ccgttgcgcg acgttgggcg cgacgacctt gatcatcgcg atttcctgcc gcgccgcctt
     4081 ggcgccgacc gtgtccatca tgtgagcggc cttgagcgtg aggagccttg cctgctcgat
     4141 caggatccgc gcctcggcga tgcgctccag cgtgacggtc tgctcggcga gcggccggcc
     4201 gaaagcgact ctgctctcgg cgcggcggca catgcgctcg agcgtccgct cgcaaaggcc
     4261 gatcagccgc atgcaatgat ggatgcggcc aggaccgagg cgcccttgag cgatttcgaa
     4321 gcctcggccc tcgccgagca ggatgttctc ggccgggacg cgcacgtcgg agaacgtcac
     4381 ttccgaggcg cgatccggca cgccgtaaaa gccgagcacc ggcaatgcgc ggacgaccct
     4441 gacgcccggc gcgtccttcg ggacgaggat catcgattgg cggcggtggc gattcggatt
     4501 gtccggatcg gtttgcccca tgaagatgat gatcttgcaa cgcggatcgg tggcgccggt
     4561 cgtgtaccat ttccggccgt tgatgacgta atgatcgccc tcgcggcgaa tcgagcttgc
     4621 gacattggcg gcgtccgacg aggcgacgtc cggctcggtc atggcgaagg cggagcggat
     4681 ttcgccggca agcagcggct tcagccatgt ctccttctgc gcctcggtcc cgtagcgcag
     4741 gatcgtctcc atattgccgg tgtccggcgc gctgcaattg aacacttccg gcgccagatg
     4801 cgagcggccc atgatctcgc agagcggcgc gtagtcgaga ttgctcaggc cgccggcggc
     4861 ggccggcagg aataggttcc agaggcccgc ggcgcgcgcg agcgccttca gttcctcgac
     4921 gaccggatag accgcccaag ggccgaggcg ctcggcctcc tggtagaaac gccgctcgtt
     4981 cggatagatg cgctccgcca tgaagcggcg gatgcgttcg gccaattcgg cgctgcgcgc
     5041 gctttgagcg agattcacgg aatgcggcct ccgatgcgat gtcgccgctc cggggagggc
     5101 tatccggaaa ggtctgcgcc tctgcggaac gactgattta ctttcgacgt cgcggcgcca
     5161 cgccgtccga tcgccgtcaa cccgccatag cgcctcgaaa gttggcgcga aagtacgaac
     5221 gcatgcgcgg cgctatggcg ggtcctccgc ttcgcgtcgg cccttcgggt gaccgcgacc
     5281 ggccggcgtg gcggcgcggc gcttgggatc tgcatccttg cacgcgcgta ctgttcgtaa
     5341 ggcgcgccaa aaagcctcca gcagccctgc gctctgcgcc gcggcgctgc gccgtcattg
     5401 ccgcgccgtc gtgccgccat cgacgacgag cacggttcct tgacaatagc tcgcttcgtc
     5461 cgagccgaga taaagacagg cgtaggccat ttcctcggcc gtgcccggcc gcttcatcgg
     5521 gcagaggcgg tcggccatgg cgggatcgtc gaacaggccg agcaccgatt cgaccatcgg
     5581 cgtcttgatg aagcccggcg cgatgcagtt agtgcggata ttgtcgccgc aataggtgat
     5641 cgccaacgag cgggtcaggt tgatcgccgc gcccttggcc gccgtatagg tatgggcgac
     5701 cggcaggcct tgaaagccgg aaatgctggt cacgttgacg atcgcgccgc cgccctgctt
     5761 cttcatttgc ggaatcgcga gccggcacat gtggaagagc gaatcgaggt tgatcgccat
     5821 cacctccgcc catttctcgg gcgtggtgtc gtcgatcggc cccatagagc cggggctctt
     5881 ttcgagccag ctatagccga cgccggcgct gttcacgagg atgtcgacgc ggccataggc
     5941 cgagagcgcc gcgtcgaagg cctttttggc cccggcctcc gtcgagaggt cggcggcgac
     6001 cacggcgccg tcgccgccgg ccgccttcac ctgcgccagg gtctcgtcga gattgctctg
     6061 cgtgcggctg accccgacga ctttcgcgcc ttcttgcgcg aacagcttca tgcaggcgcg
     6121 cccgacgccc gtgcccgccc cggtgatcag cgcgactttg cctttgaccc ggtccatcga
     6181 ttcctccctt gagcgaacgc gtccaatcga tgatggacaa tcatttcgcc cgaccttatt
     6241 gtaggaaaga tttgtaggac agaaaagcga aaatccggat cgaaccggac gccgatcttc
     6301 agggaaggac cgccatgcgc gccgccgtct ttcacgaatt gggcaagccg ctgcagatcg
     6361 aaaccgtcgc caagccgcag gccggccccg gcgagatcgt gctgaaggtc cattattgcg
     6421 gcgtctgcgg ctccgacctg cacgccaccc atccgggcgt cttcgtcgtg cccgacggca
     6481 cgattctcgg ccatgaattc gccggcgaga ttgtcgaatc cggcgcgccg ggctggaagg
     6541 tcggcgacgt ggcgaccgcg ctgccgaaca atgcgtgcga ggaatgccgc aagctcggcc
     6601 tcggcgaatg caaggacaat ctcggcatta tgtgcccgag gaacacgctg accggcttcc
     6661 atccttccgc gccgggcgcc tatgccgaat atgtgaaatt cagcgccaag gaggcgctgc
     6721 gcctgccgag cgcggtcaag agccgcgagg gcgcggcggt cgagccgctc gccgtcggcc
     6781 tgcacgcggt caatcgcggc aaggtgacgg tcggcgagcg cgtcctgatc ctgggcggcg
     6841 ggccgatcgg cctcgccacc gccgtcttct cgaagctcgc cggcgcccgc gacgtcgttg
     6901 tgagcgaatt tgcgccggcg cggcgcgagg cggccggcgc gatgggcgcg acggcggtga
     6961 tcgatccttc caaagaagag gtcggcgagg ccttcgcgcg caaagccggc gccccgcccg
     7021 acgtgatttt cgaatgcgtc ggcgtccccg gcatgatcca gaaggcgatc gacctctcgc
     7081 gcacctatgg gcgcatcgtc gtggtcggcg tctgcatggt cgaggacacg acgacaccga
     7141 tctcggcgat cttcaaggag accaacatcc agtacgtgct cggttacggc cgtccggact
     7201 ggcgcctcgt cctcgatctg ctcgattccg gccgcgtcga tccgcggccg atgatcaccg
     7261 acacggtggc gctggaagaa ctgcccgccg cgttcgaggc tctgcgcaag ccgacgacgc
     7321 aaatcaaggt catggtccgc cccggcgagc ggagcggttc tttgtagggc aagattgttc
     7381 cttgataagc acgggatcgc ccgctaagga ggccgcgccg gcccggacgt ccgggcttcg
     7441 aagcggggct atggaacaca acgtgatgga cagggacgtc gccgcgccgg cggtcaagcc
     7501 tcgcgccagc ggccgcagcg cggccgaggc cgccgagcgc gccaagatga cgtcgcccga
     7561 ccgcgtcgtc gacgcgatca tccgctccat tcgcgccggc acgtacgtgc ccggccagcg
     7621 gctgatcgaa gccgacctga cgcgcgatta ccgcgtcagc cgcggcccgg tgcgcgaggc
     7681 gctgaagcgg ctcgccgccg aaggcgtgtt gaccttgacc cgccaccgcg gcgcctatgt
     7741 gcgcgcgctg tcgcggatcg aggtgcgcga cagtctgatg gtgctcgaag cgctggtcgg
     7801 gctgatggcc aatctggcgg caaggcgcat cgaagaggac gacaacgccg cgcgcatgcg
     7861 cgaagcctat cagcggctgc tcgccttcaa gcatgacggc ggccccgccg cctttctcga
     7921 cgagcggcgc gccttctacg acacgatcct gcaaatcggc ggcaaccgcg agctgaagcg
     7981 tatgctgcct ctcatgcaga tccatctgct gcgcatgcag ttccaggcct acatcacgcc
     8041 gcgcgaccgc gagaagcagt tcaaggaata tgagacgatc accgagacga tcctcgccgg
     8101 cgacagcgtc cgcgcccaga aagtggcggc tttgcacgtc cgtcgcaccc gtctcagcct
     8161 gatgcgcctg cccgacgagg cctttccggc catacgcagc gcggactgat tgaatgacca
     8221 tcctgatcgc cgggggcacc aagggaatcg gcctggcggt cgccaaggcc ttcgcgcctg
     8281 aagcgggcga cgtgtttctc ggctatcaca gcgacgatga ggcggcgcaa gaagcggccg
     8341 cgtcggtcga agcggccggc gggcgcccgc atctcgtcaa ggccgatgtc gccgcgccgg
     8401 aaggctgcgc cgcgatggcc gaagccgtgc ggacgagcgc cggccggctc gaccagctcg
     8461 tccattgcgc cgtcgacgcc tacgcatcga gcgcgctcgg cgccgatccg gcgcgcttcg
     8521 cgcgcgccat ttccgccaat ggcgccagtc ttctctatct cgtgcaagcg gctctgccgc
     8581 tgatgccgcg cggcgccagc attttctatc tgaccagccg cggcggccgt atcgtcgtac
     8641 ccaattacgc cgctgtcggc gtggcgaagg cgctggccga gagcctgatg cgctatctcg
     8701 ccgtcgagct cgcgccgaaa ggcgtccgca tcaacgccgt cgcgccggcg atcgtcgaga
     8761 ccgacgcggt tcgcgccttg ttcggcggcg aagccgccaa tctggtccgc gcggcggccg
     8821 agcacaatcc gtccggccgc ggcgtgaccg atgcggacta caccaacctg atgcgctggc
     8881 tcgcctcgcc cgaagcgacg ttcatccagg gccaggtcat cttcgtcaat ggcggcgcca
     8941 atctgagcgc ttgagcgtca caatcgctcg atcaaagtgg cgttggccat gccgcccgat
     9001 tcgcacatcg tctgcacgcc ggtgcggccg cccgtctctt cgagccgcgc gacgagattg
     9061 gcgagcaggc gcccgcccga ggcgccgacc ggatggccga gggcgatcgc cccgccatag
     9121 gcgttcaccc gctccggatc ggcgccggtc tccttcagcc aagccagcac gaccgaggcg
     9181 aaggcctcgt tcacctcgaa cgcgtcgatc cggtcgacgc tcaggccggc gcgccgcagc
     9241 agcttgcgcg tcgccggcac gatcgcggtc agcatcagga tcggatcgtc gcccgccagg
     9301 gcgaaatggg tgattgcggc gcgcggcttc aggccgagct tgcgtgcgac gctttcctcc
     9361 gcgatgagca cggccgcggc gccgtcggtg acctggctcg aattgccggc ggtgacgctc
     9421 cagcggattt gcggaaaacg cgccgccatt gcggcgtcct cgaaggccgg cttcagcgcg
     9481 gcgagctttt cgatcgaagt gtcgcggcgc ggcccttcgt cggtctcgac gatgccggcg
     9541 gcgccgtcgg cggcttgcgt ctcgatcggg acgatggcgc gccgggtcag gccggcctcc
     9601 tcggcggcga tcgcgcggcg gtgcgactcc agcgcgaagc gatccatcgc ttcgcgctca
     9661 atgccccagc gcgcggcgat gagctccgcc gacacgccct ggcgcaccag cccttgcgga
     9721 tagcggcggt ggaaggccgg cccctcattg tccttgccca tgcggttgag gcgcatcggc
     9781 acgaggctca tcatctcgac gccgccggcg atcacgacgt cataggcgcc ggcgacgacg
     9841 ccttgcgcgg cgaaatcgaa ggcttgctgc gccgagccgc atttgcggtc gagcgtcacc
     9901 gccggcgtgc tttccggcag gccggcggcg agcgccgcct ggcggccgat attgccggcc
     9961 tgttcggcga cctgaaggac gcagccggtg atcacgtcct cgatcagcgc cggatcgacg
    10021 cccgtgcggc gcaccagggc cgccagtacg ctcgcgtaga gatcgaccgg atgcagcccc
    10081 gacaaggcgc cgcccggccg gccgcgccca aacggcgagc gcacgacgtc gacgatcacc
    10141 gcgcggcgca tgtcatctcc actccgcctg atccgtcctc acgctcgttt caacccgtct
    10201 ggcgctgcag atcgagcgac agtctggccg tgcgaatctg gacgacgggc gatttcgcgg
    10261 cgcgcatcag cttctgcacc tgatcgagct cgggcgaaga gaggagcgga tagtcgcgcc
    10321 gcaccggccc ggccgcgcgc attttctcca ggtccgattc cggcaccagc gcacgcagga
    10381 tcagctcgtc gtcgtcatcg acgccgtagc gcctgcgcag ctcctcgatc gtcggctgct
    10441 ccggcgggct gcccgcaacc tgcttggcgc gcggcgcggc catgatcatg tcgagcacgt
    10501 tcggatcgat cggcgcgacc gtctggccat agaagccggc cgcgtattgg atcacctcgt
    10561 cgggaatgat gctgtagcgc tttcccgtca ccacattgag cacggcttgg atgccgacaa
    10621 gctggctgaa cggcgtcgcc atgccgggat agccgagatc gcggcgcacc tcggcggtct
    10681 cgcgcagcac ttcgtcgagc ttgtcggtca tgttgtgctg ggcgagctgg gccttcagcg
    10741 ttccggtcat gccgccggga atctggtggc ggatcgacag gacgtcgtat tcgttgtgct
    10801 gattgaccgg gaagccggcg gccttgccga ccttctcgaa atgctcggcg atggccggca
    10861 gcagcgcctt gtcgacgccg tgggtatggc cgagcagttc gagattgtgc agcatgatct
    10921 cggtcgacgg aaccgagggg ccgttcgcca taggccggct cgccgtatgc aggatcgcga
    10981 cgccgagatc gatcgcgtcg agataggcct tcgccgactg gccgagaata ttgttggcgt
    11041 gaaactcgat cggcttgccg cgcgctttcg ccttgatcgc cggcacaaga gtcttcagcc
    11101 gctccttgtc gagcacgccg gccgtatcat agagcagcag cgtgtcgata tcgggcgagg
    11161 ccgacagctt gtcggccttg tcggcatagt attcgtcggt atggacgggc gacagcgcga
    11221 acatgatcga gccggcgacc tccgcgccga attccttggc gactttggcg agccggcgca
    11281 tcttgtcgat gttgaagagc acgtcataga tccagaagct gcggcagcca tgccggttga
    11341 gctggcgcat ccaggcgtcc atgagggaat ccggcgtgac gccgaacgtc accgaggcgt
    11401 tcgagcgcat gccgccgcga atgcgcgtgc gcggcatcgc cccaaccagc agatccagcc
    11461 cttcccaggg attttcgcgg caagtccgga tcatcacttc gaacagggac gaaccggcga
    11521 gatcgatgac gcgaaagccg cagcggtcga tcagcggcgc gacgggaagc gccatgccgg
    11581 cttgcatgcg catgccccaa aggctctgct ggccgtcgcg catggtctca tccagcaact
    11641 cgatatgagc catggtctat ccctctggct ggccgtaatg cggcaggaaa acattttcga
    11701 gccaacgcgt gtcgagccgg gcgccgatga agtcggcatg gccggcgatg gcgcgcagca
    11761 gggcgatgtt ggtggcgacc ccctcgacct caaaggagtc aagcgcctcg cgcagccgcg
    11821 ccacggcgcg cggccggtcg ggcgcatgaa cgatcagctt ggcgatcatg ctgtcgtaat
    11881 agggcgagac gacggagcct tcctcgatcg ccgtgtcgag ccggacgccc tcgccttgcg
    11941 gcgggcgcca gcggacgatg cggcccggcg agggcaggaa gccgcgctgc ggattttcgg
    12001 cgaggatgcg cgcctcgatc gcatggccgt gctggacgat gtcgccctgc gcgataggcg
    12061 gcggcgcgcc gccggcgatt tcgagctgca ggcggatgag atcgacgccg cagatcatct
    12121 ccgataccgg atgctcgacc tggatgcgcg cattgacctc catgaaataa aaatcgtcgc
    12181 gctcgacatc gtaaaggaac tcgaccgttc cggcgttgcg atagccgatc gaggcgagca
    12241 gggcgaccgc cgcttcgtgc aggcgcgatc gggtcgcagc cggcaacgcg gcggccggcg
    12301 cctcctcgat catcttctgg tagcgccgct gcatcgagca gtcgcgctcg ccgaaatgca
    12361 gaacgcggcc atcgccgaag cccatgacct gaacctcgac atgccgggcg cgctcgacat
    12421 agcgctcgag aaacagcgtg ccgtcgccga acgcctcctt ggcctcggcc gaggcgcgcg
    12481 cgaagccgtc gcgaagcgat gtcgcgtcgc gcgcgatgac catgccgcgg ccgccgccgc
    12541 cggccgacgc cttgatcagg gcgggaaagc cgatcctgtt cgccgcggcg accgcctcct
    12601 cctctgtcgc gagctgtccg ctgccgggcg tcgtcgcaac gccggccgcc gtcgcgaggc
    12661 ggcgcgccgc cagcttgtcg ccgaccgctt cgatcgagtc cgaagaagga ccgacgaacg
    12721 cgattccat
//
LOCUS       DATGJW010000445         5018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1331361, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000445 DATGJW010000000
VERSION     DATGJW010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5018
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1331361"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(240..851)
                     /locus_tag="VKU03_13350"
     CDS             complement(240..851)
                     /locus_tag="VKU03_13350"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ72300.1"
                     /translation="MDQPLAPHATIRRLWEADRELFRGHLLRLDPESRHDRFNGGASD
                     EFLHRYVDRCFAPGDVIFGAFVDGELRGAGELRPLEAEAARPRFFAAPGKAEAAFSIE
                     RPYRRHGLGVQLFERLMRAAAAHRIGEIDFTCASDNKAMQGLARKFSAEMHFAANQIT
                     GRLTARHATAFSIWREAVSDASDYAMAMLDAQKRALDERQAKT"
     gene            complement(972..1949)
                     /locus_tag="VKU03_13355"
     CDS             complement(972..1949)
                     /locus_tag="VKU03_13355"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003599947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003960 - NADPH:quinone reductase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0070402 - NADPH binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH:quinone oxidoreductase family protein"
                     /protein_id="HLJ72301.1"
                     /translation="MKALLCHEYGPPERLAIADLPEPSPGPGEALVEVGFAGLNFFDL
                     LVIENKYQAKPPLPFSPAAEFSGRVLALGPGAKGFAPGDRVLGYAGFGCARERLVCPV
                     DRLVAIPDGLADEKAAGLTITYGTTLHALKQRADLKRGETLVVLGASGGVGLAAVELG
                     ARLGARVIACASSDEKLKFVRSFGAAETINYATGDLRAELKRLTGGRGVDIVYDPVGG
                     ALTEQALRSLAWKGRLLVIGFASGEIPKPPLNLALLKGCDIRGVFWGEFLNREPEAHR
                     RNMAELLADAASGAISAHVHAVYPLESYLDAFGAIARREALGKVILKLG"
     gene            2104..2790
                     /locus_tag="VKU03_13360"
     CDS             2104..2790
                     /locus_tag="VKU03_13360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72302.1"
                     /translation="MIRFALLPALVATAILAVAAGPALAQQSSAASDAKKKAARAHPA
                     KEKDKDKAEKEKGDGKPLLVASFGDWGVYQSQAGKGRVCYTLAQPKDREPADLKRDPA
                     YAFISDRPAEGVRNEVSFIMGFDVANPPDESLDASDEKAAKGKKRKPTIRGATATVGD
                     QSFDLLAKATNLWVKNAAKEGQLVDEMRKGAKLEVKAMSKKGNLTTDTYSLAGFSQAM
                     ERVLKDCPVR"
     gene            complement(2783..3544)
                     /gene="ubiG"
                     /locus_tag="VKU03_13365"
     CDS             complement(2783..3544)
                     /gene="ubiG"
                     /locus_tag="VKU03_13365"
                     /EC_number="2.1.1.222"
                     /EC_number="2.1.1.64"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008689 - 3-demethylubiquinone-9
                     3-O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006744 - ubiquinone biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional 2-polyprenyl-6-hydroxyphenol
                     methylase/3-demethylubiquinol 3-O-methyltransferase UbiG"
                     /protein_id="HLJ72303.1"
                     /translation="MNAADAPSIDPDDVARFEALGEDWWDPAGSMRALHRINPLRLGW
                     IRDLLIRRFGPAGADAPGPLAGLAILDIGCGAGLLSEPLARLGAEATGIDPAPGNIEI
                     ARRHAEETGADVLYRAASLEEVAAEGKLFDAVLAMEVVEHVRDMRGFVKMACGLVKPG
                     GLFAASTLNRTFRSFALAIIGAEYVLGWVPKGAHRWEQFVTPDELREACRAAGCREIA
                     RTGMIYDPLQRRWRLSRDASVNYMMAARKSEAAAT"
     gene            3676..4914
                     /locus_tag="VKU03_13370"
     CDS             3676..4914
                     /locus_tag="VKU03_13370"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012634396.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HLJ72304.1"
                     /translation="MARLVMKFGGTSVANIERIRNAARHVKRERDAGHEIAVVVSAMS
                     GKTNELVGWCAEAAPLYDPAEYDAIVASGELVTAGLLAIILKDAGIPARSWQGWQIPI
                     LTNDAHGAARIADIESAGLLAGFGRGEVAVVAGFQGLHKPTNRVTTLGRGGSDTSAVA
                     LAAAVKADRCDIYTDVDGVYTTDPRIVPKARRLEKVAFEEMLEMASLGAKVLQVRSVE
                     LAMMHRVKTYVRSSFDDPLAPNSGTLICDEEDIVEQQIVTGIAYSTDEAQITLRRVAD
                     KPGVAAAIFVPLADANINVDMIIQVVSDDTTTTDITFTVPSAEFRRAKAILEERRDAI
                     GFSSLQGAEDVVKVSAIGVGMRSHAGVAAKAFKALAEKGVNIRAITTSEIKFSVLIDA
                     AYTELAVRTLHSLYGLDAAA"
BASE COUNT          888 a         1675 c         1671 g          784 t
ORIGIN      
        1 cgatcttcgt actttggaga agcgtccggg cgctcggatt gctccgaggc tcgaaaatcc
       61 caaacgctgc gccgccgccc catcgcccga agggccggag cgaagtcgag gatccgcccc
      121 agcgccgcgc cagcgtttgt actttatcgt gaactttcgc tggcgccatg gcgggctgat
      181 cgggcgtcgt ggcgtcgcgg cgctgaaagc gcatcagtcg cgtcgcccgc ggcgtcccac
      241 tacgttttcg cctgccgctc gtcgagcgcc cgcttttgcg cgtcgagcat cgccatcgcg
      301 tagtcgctcg cgtcgctcac cgcttcccgc cagatcgaga aagcggtggc gtggcgggcg
      361 gtgagccgcc cggtgatctg gttggcggcg aaatgcattt cggccgagaa tttgcgcgcc
      421 aggccctgca tcgccttgtt gtccgatgcg caggtgaaat cgatctcgcc gatgcgatgg
      481 gcggcggccg cccgcatcag gcgctcgaaa agttgaacgc cgaggccgtg gcggcgatag
      541 ggccgctcga tcgaaaacgc cgcctcggcc ttgcccggcg cggcgaagaa gcgcggcctc
      601 gccgcttccg cctcaagcgg ccgcaattcg ccggcgccgc gcaattcgcc gtcgacgaag
      661 gcgccgaaaa tcacgtcgcc cggcgcgaag cagcgatcga catagcgatg aaggaattcg
      721 tccgaggcgc cgccgttgaa ccggtcatgc cggctttccg gatcgaggcg caggagatgg
      781 ccgcgaaaaa gctcgcggtc ggcctcccac agacggcgga tcgtcgcatg cggcgccagc
      841 ggctggtcca tggcggtttg tccccgttct gaagcctctc cctcgccgca gccgatatgg
      901 cgaggcggcg cgccggcgca aagcccaaag ccgcatggca gaacggcgtt gcgcgctgga
      961 cggggcggcc ctcagccgag cttcaaaatc accttgccca acgcctcgcg gcgggcgatc
     1021 gcgccgaaag cgtcgagata ggattcgagc gggtagaccg catggacgtg agccgaaatc
     1081 gcgccgctcg cggcgtcggc cagcaattcg gccatgttgc ggcgatgcgc ctcgggctcg
     1141 cggttgagga attcgcccca gaacacgcct ctgatgtcgc agcccttgag cagcgccagg
     1201 ttcaacggcg gcttgggaat ttcgccggaa gcaaagccga tgaccaaaag ccgacccttc
     1261 caggcgagcg agcgcagagc ctgctcggtc aaggcgccgc cgaccgggtc atagacgatg
     1321 tcgacgccgc ggcccccggt caggcgcttc aattcggcgc gcaaatcgcc ggtcgcgtaa
     1381 ttgatcgtct cggccgcgcc gaacgaacgt acgaacttca atttttcgtc ggacgacgcg
     1441 caggcgatca cccgcgcgcc gagccgggcg ccgagttcga cggcggccag gccgacgccg
     1501 cccgacgcgc cgagcacgac caaggtctcg ccgcgtttca gatcggcgcg ctgcttcagc
     1561 gcgtgcagcg tcgtgccata ggtgatggtg aggcccgccg ccttctcgtc ggcaaggccg
     1621 tccggaatcg cgacaagccg atcgaccggg cagaccagcc gctcgcgcgc gcagccaaag
     1681 ccggcatagc cgagcacgcg gtcgccgggc gcgaaaccct tggcgcccgg gccaagggca
     1741 aggacgcggc ccgaaaattc agccgccggc gaaaagggca gcggcggctt cgcctggtat
     1801 ttgttctcga tcaccaggag atcgaaaaaa ttgagcccgg cgaaaccgac ttcgaccagc
     1861 gcctcgcccg ggccagggct aggctccggc aggtcggcga tcgccaggcg ctccggcggt
     1921 ccgtattcat ggcaaagaag ggctttcatc gaaggcctcg gggaggttaa tcctccttaa
     1981 ggaaagcagg tttacaagcc tgttgcccct gatggccagg ggcggggcag tcagaattcc
     2041 gccccgatcg ttgctatttt gaagcgcggc tgcgcccatc tgcccgccgg agtgcctaga
     2101 accatgattc gctttgcgct tttgcccgct ttggtcgcga cggcgatcct cgccgttgcg
     2161 gcgggtcctg ctctggctca gcagagctcc gcggcaagcg acgccaagaa aaaggcggcc
     2221 agggcgcatc cggcgaagga gaaggacaag gacaaggcgg agaaggagaa gggcgacggc
     2281 aagccgctgc tcgtcgccag ttttggcgat tggggcgtct atcagagcca ggcgggcaag
     2341 gggcgcgttt gctacacttt ggcccagccc aaggaccgcg agccggccga cttgaagcgc
     2401 gatccggcct acgccttcat ctccgaccgg ccggccgagg gcgttcgcaa cgaagtctcc
     2461 ttcattatgg ggttcgacgt ggccaacccg ccggacgaga gcctcgacgc ttccgacgaa
     2521 aaggcggcaa agggcaagaa gaggaagccg acgattcgcg gcgccaccgc aacggtcggc
     2581 gatcaatcct ttgacctgct cgccaaggcg accaatcttt gggtgaagaa cgccgcgaag
     2641 gagggtcagc tcgtcgacga gatgcgcaag ggcgccaagc tcgaagtcaa ggcgatgtcg
     2701 aaaaagggca atctgaccac cgacacctat tcgctggccg gcttctcgca ggcgatggag
     2761 cgcgtgctca aggattgccc ggtcaggtag cggccgcctc gctcttcctg gccgccatca
     2821 tgtaattgac gctggcgtcg cgcgacagcc gccaccgacg ctgcagcgga tcgtagatca
     2881 ttccggtccg ggcgatctcg cggcatcccg cggcgcggca ggcttcgcgc aattcgtccg
     2941 gcgtgacgaa ttgctcccac cgatgcgcgc ccttcgggac ccagccgagc acatattccg
     3001 cgccgatgat cgccaaggcg aagctgcgga aggtgcggtt gagggtcgag gcggcgaaga
     3061 gtccgcccgg cttgaccagg ccgcaggcca ttttgacgaa gccccgcatg tcgcggacat
     3121 gctcgaccac ctccatggcg agcacggcgt cgaaaagctt tccctcggcg gcgacctcct
     3181 cgagactcgc cgcgcgatag agcacatccg cgccggtctc ttcagcgtga cggcgggcga
     3241 tttcgatatt gcccggcgcg gggtcgatcc cggtcgcttc ggcgccgagc cgggccagcg
     3301 gctcgctgag gaggccggcg ccgcagccga tgtcgagaat ggcgaggccg gcgagcggcc
     3361 cgggcgcgtc ggcgccagcc ggcccgaagc gcctgatcag gaggtcccgg atccagccga
     3421 ggcgcagcgg gttgatccga tgcagcgcgc gcatcgagcc ggccgggtcc caccaatcct
     3481 cgccgagggc ctcgaaccgg gcgacatcgt ccggatcgat gctgggcgcg tcagcggcgt
     3541 tcatcctgcc tccagatcgg ctcgaaaagc ggcgcggcgg acgttgacgc ggccggctcg
     3601 cgccctctat acgccggctt tcgggacagc gcgaccggct cgcgcaaagc gagttacgat
     3661 aggggcttag gggccttggc gcgtctggtg atgaaattcg gcggcacgtc ggtcgccaat
     3721 atcgagcgca tccgcaacgc cgcccgccat gtcaagcgcg agcgcgacgc cggccatgaa
     3781 atcgcggtcg tggtctcggc catgtcggga aaaaccaacg aactcgtcgg ctggtgcgcc
     3841 gaggcggcgc cgctctacga cccggccgaa tatgacgcga tcgtcgcctc gggcgagttg
     3901 gtgaccgccg gcttgctcgc gatcatcctg aaagacgccg ggattccggc gcgctcctgg
     3961 caaggctggc aaatcccgat tctcaccaac gacgcccatg gcgcggcccg gatcgccgat
     4021 atcgaaagcg ccggccttct cgccggcttc ggccgcggcg aggtcgcggt tgtcgccggc
     4081 ttccagggtc tgcacaagcc gaccaaccga gtgacgacgc tcgggcgcgg cggctccgac
     4141 accagcgccg tcgccctcgc cgcggcggtc aaggccgacc gctgcgacat ttatacggat
     4201 gtcgatggcg tctatacgac cgacccgcgc atcgtgccca aggcgcgccg gctggagaag
     4261 gtcgccttcg aggaaatgct ggaaatggcc tcgctcgggg ccaaggtgct gcaggtccgc
     4321 tccgtcgaat tggcgatgat gcacagggtc aaaacctatg tgcgctcgtc cttcgacgat
     4381 ccgcttgcgc cgaattccgg cacgctgatc tgtgacgagg aggatatcgt ggagcagcag
     4441 atcgtcaccg gcatcgccta ctccaccgac gaggcgcaga tcaccttgcg ccgtgtcgcc
     4501 gacaagccgg gcgtcgcggc ggcgattttc gtgccgctcg ccgacgccaa catcaatgtc
     4561 gatatgatca tccaggtcgt ctccgacgac acgaccacga ccgacatcac cttcaccgtg
     4621 ccgagcgccg aatttcgccg cgccaaggcg attctcgaag aacgccgcga cgcgatcggc
     4681 ttttcctcgc tgcagggcgc cgaggacgtg gtgaaggtct ccgccatcgg cgtcggcatg
     4741 cgcagccatg ccggcgtcgc cgccaaagcc ttcaaagcgc tggccgaaaa gggcgtcaac
     4801 atccgggcga tcaccacctc ggagatcaag ttctcggtgc tgatcgacgc cgcctatacc
     4861 gagctcgcgg tgcgcacgct gcactcgctc tacgggctcg acgccgcggc gtaatccgag
     4921 acgcgaggtc cggatacctt taccagcgtg cgcgcgctgc gtgacggacg ctgtgaatga
     4981 cgacctcgat tccagagatg cggtagaaga tcacatac
//
LOCUS       DATGJW010000446        16432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_708658, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000446 DATGJW010000000
VERSION     DATGJW010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 16432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16432
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_708658"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            205..1110
                     /locus_tag="VKU03_13375"
     CDS             205..1110
                     /locus_tag="VKU03_13375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019172872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HLJ72305.1"
                     /translation="MTAVPAPVASAEARERAAELAAFWAVYRENKGAVFGLCVLALIV
                     LCAIFANVIAPYSPIEQFRDAIRAPPVWDGGTWRFALGADGLGRDILSRLIYGARISL
                     FIGLAVMGLSSALGVALGLSAAYFGGLIDVAVSRLTDVIWALPSLVLAVLVIAIIGPS
                     LFNTIIAIAIVYTPTFVRLMRASALAELSKDYVVAARAVGVGPLRLMLATVLPNCLAP
                     YIVQAAIGVSSAILEAAGLGFLGLGAQPPTPEWGAMLADAREFIRSDPWTVTLPGLAI
                     VVTVIAINLTGDGLRDALDPKMRRS"
     gene            1113..2081
                     /locus_tag="VKU03_13380"
     CDS             1113..2081
                     /locus_tag="VKU03_13380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72306.1"
                     /translation="MALLDIRNLSIAFATRHGPFKVVDGIELSVERNEVLAIVGESGS
                     GKSVAMLAVMGLLPHTASVTADRLAFEGRDLRTLSPKERRAIIGKDIAMIFQEPMSSL
                     NPCFTAGFQIGEVLRTHLGMNRAARKRRVFELLEEVGIADPERRARAFPHQLSGGMSQ
                     RVMIAMALACRPKLLIADEPTTALDVTIQAQILDLLLKLRRQNGMGLVLITHDIGVVA
                     ETADRVAVQYAGLQVETNTARALFADPHHPYAAALLAALPERANGRRLPSIPGVVPGQ
                     FDRPSACVFSPRCSFAFERCRREAPRPASAALGRALCHTPLIGGVS"
     gene            2075..3040
                     /locus_tag="VKU03_13385"
     CDS             2075..3040
                     /locus_tag="VKU03_13385"
                     /EC_number="7.4.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007066470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide ABC transporter ATP-binding protein"
                     /protein_id="HLJ72307.1"
                     /translation="MVTAALQARGLSRDYALSRGLFTPRMTVKALVDASFDLDAGKTL
                     AVIGESGSGKSTLARLLTLLEQPTAGALTIDGEDAIGAGAETRKRLRRQIQIVFQNPY
                     GSLNPRQRIGKALEEPLLVNTNLSAAERENAARAMMEKVGLRPEYYRRYPHMFSGGQR
                     QRIAIARALMLRPKILVLDEPVSALDVSIRAQTLNLLAALQEEYRLAYVFISHDLSVV
                     RHIADEVMVVYLGHVVEKGARAAIFSAPQHPYTRALLSATPVADPAAQRERIILSGEL
                     PSPFHPPSGCPFHPRCPAAMDRCRAENPALEQKQGRDVACWAVAA"
     gene            3219..4832
                     /locus_tag="VKU03_13390"
     CDS             3219..4832
                     /locus_tag="VKU03_13390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018699346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLJ72308.1"
                     /translation="MNDIRTFDYIIVGAGTAGCLLANRLSADPRNRVLLIEAGGRDNW
                     IWFHIPVGYLFAIGDKRADWLFKTAAEPGLNGRAIAYPRGKVIGGCSAINGMIYMRGQ
                     AADYDGWRQLGLEGWGWDDVLPYFKKHEDYHGRADALHGVGGEWRIERQRLHWPILDA
                     VREAAAEIGIRKTNDFNSGDNEGSDYFDVNQKRGLRWSAARGFLKPILHRQNLTLVTH
                     AQVGQILFADKRAVGLRYKGEGASIEARAAGEIILAAGAIGTPHLLELSGIGRPEILA
                     RVGAPLVHALPGVGENLQDHLQLRTIFKVSGARTLNMDYKSLVRRALMGLDYAFRRRG
                     PLTMAPSQLGIFAKTSPDYATANVEWHVQPLSLDRFGEPLHRFPAITLSVCNLRPASR
                     GSVHAASADSSAAPEIKPNYLSEEEDRRVAVDAIKLTRRIAAAPALASYKPEELLPGA
                     SKASDEDLAKAAGEIGTTIFHPVGTAKMGTQDDPAAVVDAQLKVRGLRGLRIADASVM
                     PRITSGNTNSPVLMIAEKAAAMILEEAKR"
     gene            4829..5329
                     /locus_tag="VKU03_13395"
     CDS             4829..5329
                     /locus_tag="VKU03_13395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MmcB family DNA repair protein"
                     /protein_id="HLJ72309.1"
                     /translation="MSVEGFAEPPASPYEPGLADGRQSETALFIARGLRRMLRAHGYS
                     SVTELPLLDGRRADVVALRGDGSLAIIEIKSSVADFRADGKWRDYLDHCDRLYFAICD
                     TIPPEIMPREAGLIIADAYGAAIVREAPEQRLAPATRRAVLLRFAQAAADRLHRLADP
                     GGLADR"
     gene            complement(5441..6856)
                     /locus_tag="VKU03_13400"
     CDS             complement(5441..6856)
                     /locus_tag="VKU03_13400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590941.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004497 - monooxygenase activity [Evidence
                     IEA];
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016705 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLJ72310.1"
                     /translation="MSVSDLAKPSPLGPRPPAPIPNARPLGPLSLLIRFWRNPLDTWT
                     RFNFEWPITQANGLLGRIAVVCEPAAIKRVLIDNAANYPKDPLQLRVLSPGLGDGLLT
                     SEGGDWRKQRRALAPLFTPRMVLSFAPAMVATAEWLIERWALLRPGRRLDIAGEMSVA
                     TLEALQRTIFPSGLSREPRDFARGMSELVTALGALHPFDLLDAPEWLPRVGKRSPVKA
                     LKFFDAAVDDILAARRAHGAPPPAEQGSPRDLLTMLLDARDPQTGEGLSEVEVRANVV
                     TFIGAGHETTANALTWSLFLLSQHPAWREEVEREVDDALGERPLDVEAIERLPRVRAT
                     LDEAMRLYPPAPSLSREALAADMLAGQKIHPGTLIVISPYVVHRHKLLWRDPDHFDPT
                     RFMPGEREKIDRFAYLPFGAGARSCIGMPFALQEATIFLAAILRSYRLDLAPGHKVAP
                     VQQVTLRPKGGMPMVLQHRRR"
     gene            7021..8325
                     /locus_tag="VKU03_13405"
     CDS             7021..8325
                     /locus_tag="VKU03_13405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183408.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLJ72311.1"
                     /translation="MNMRFGLSMTALAVTMLASAGMASADPVAISFLTHWPPETVAKL
                     KAATDAYSKAHPDVKIDIRAVPFGDLLTTIRSQGASANGPTIDGIYDLWLPELVRDKL
                     AAPAPADLATDVAKNWPKGVATAASIGGALYGIPNEIDLYTLNYNKKLFAEAGVAGPP
                     KTWAELEADAEKLTKKSGGTTTQQGFGLINSWAAGVVHPFASLLASNGGALIVDGKPA
                     LDSKQAGETFALYEKLIKSGASVPAMGTADANTTGPFLDNFVSGKTAMIIMANWWESA
                     LKAGMKDNFKDIAAAPIPVGPSGDAPHSISYSWMTVVNAKAEPAAQKAAWDFLAWLNG
                     PTSGPHGASAMGDILMSMGILPSRTSDVAAFKDQLSSPFLSAYVAALPEARPFPDVLG
                     GQEFTEALQQHIEAIEFGKESAAAAQKAAQEDAVSILTRAAK"
     gene            8635..9543
                     /locus_tag="VKU03_13410"
     CDS             8635..9543
                     /locus_tag="VKU03_13410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HLJ72312.1"
                     /translation="MSRVMIRSGAFLLPRIALLAPGVGLIAAFMFAPILLTVWLSFHA
                     WSTQTPFSAARFVGLANFADLFGPTSVGRDFRGAFANTALYAILSIALILPLSAALGV
                     LIHQSRAIGRNALRAVLFSTYMVPSIAVALVWSKLYSPTEGPYDQMLAWLGLPTSDWL
                     SSPNTALVSLVLLNVWQQTGYFTVLAVAGLTQIPTTVYEAATLDGANRWQAFGHVTLP
                     LLRRTLLFSSVIALINAVQVFEPVALITQGGPVGATNVLTYHIRRVGVERAQGGLGSA
                     MAVMLLLTLIATVAVLFAAARERRGP"
     gene            9540..10430
                     /locus_tag="VKU03_13415"
     CDS             9540..10430
                     /locus_tag="VKU03_13415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019856765.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HLJ72313.1"
                     /translation="MSGAAVSTATRPIALPRPRRRRDAPTQIALAGLVIAIGLAAAFP
                     LAWMALSSLKTPSETMQTPPVWWPATPSLDAYSKVANVIEAGRSFLNSAIIASAATLG
                     ILVTSLMAGYAFAKHQFRGRDALFAVIVATMFLPPIVTLIPLYRMVGSLGLDGSLLGV
                     ILPNLANAFGIFLMRQFIAGVPNELIEAARIDGASELRIVFGIVAPNVLPAIATLAIF
                     AFCYHWNSYLWPLTILQGNGDAYPIVLSLSRLLSYNRGAMNTNLVMAGATLAVLPPLL
                     LFIALQRAFISSIIQSGLNG"
     gene            10427..11308
                     /locus_tag="VKU03_13420"
     CDS             10427..11308
                     /locus_tag="VKU03_13420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018428023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HLJ72314.1"
                     /translation="MSRVLALDLGGTHLRAGLAAANEAASVQTIGRWPAPRDMESFRV
                     LLGEIVASRGPFDRVGLAVPGLAEETRCRWIPNLPWLDGVDLADLVAGAPLALGNDSQ
                     LALLAEATHGAAAGLSDAILLSIGTGIGSAVLSGGRIVKGAAGGACSFGWACADMADQ
                     GRHKDGWLERMAAGRAFDAAAVKTFGAADGAALIAAARAGDQAAIEAIAKPAAAIGTA
                     LAGAVALLDPQAILFTGGVAESLDALAPAILAALRRHLPPHLKRVALRAGRFSGSAAL
                     IGAAIAGAAGRDWRSLR"
     gene            11305..12069
                     /locus_tag="VKU03_13425"
     CDS             11305..12069
                     /locus_tag="VKU03_13425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ72315.1"
                     /translation="MTAIFPLPDRRRPEPLWHQCERSIQALIESGEWAAGAQIPAEDR
                     LCAMLGVSRITIRHALRNLEESGLLRREHGRGTFVRSATLIAGARGLTSFTEEMSSLG
                     LAAGSRLLDQEVIAASADIAAALDIEEGASVLRIRRLRLGGDAPIGVQTAHLPLDRVA
                     ALLGAGPLDGSLYAALETRCGIKPEEARETYRVALIDGADAALLGVAPGSPVFVVERI
                     TADGRGAFEFTLSLMRGDRYEIRSTLRAKPFSQERL"
     gene            12066..12860
                     /locus_tag="VKU03_13430"
     CDS             12066..12860
                     /locus_tag="VKU03_13430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015316346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar isomerase domain-containing protein"
                     /protein_id="HLJ72316.1"
                     /translation="MTDLYISRLTDLIEKASKTNAATFDSVAKRLAETLQNGGLIHLY
                     GSGHSVLPVQEAFPRYGSYVGFNPLTDPRVMWHNVLGAGGVRELLWLERTEKYAEKFL
                     DHQPLNAGDCIIIYGHSGRNASGIDAALYAKKRGLFVVAITAKANLDKPATHSSGKRL
                     ADAADAVIDTCAPIEDAIVPIEGWSRPVSGSSTVLAMIMTHELIARTAQALAKRGVEL
                     PTFASPTIAGVTLHDTDVIYGVYRDRLIEAQRKHLPAFQAKMRGEG"
     gene            13012..13800
                     /locus_tag="VKU03_13435"
     CDS             13012..13800
                     /locus_tag="VKU03_13435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72317.1"
                     /translation="MPPLLEVADLHKSFGPINVLRGVDLSLNAGEVLALVGDNGAGKS
                     TLIKLISGVHKPDSGEIRLNGAPLDVHGPADARRLGIETVYQDLALADDLSVGANVFL
                     GREPVRHWLGLLPAIDNRKIRIETDSLLKRIESRIPPNARTVSRLSGGQRQAVAIARA
                     LYWQSKVVILDEPTAALAVMEREHVIQLARRIAEEGIGVIYIGHNLIEILEVADRVAV
                     MFQGRIVHVTPVAETSQRDLIKYMTGFTESKEALSHEGRTQGRR"
     gene            13803..14792
                     /locus_tag="VKU03_13440"
     CDS             13803..14792
                     /locus_tag="VKU03_13440"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ72318.1"
                     /translation="MLKLTLLAATALALAAGPAAAADFSKDRIPVIVKATTSQYWATV
                     FDGADAAAKALGVQISKLGASAETDVAQEVSIMENAIQSKPTAIVIAATNAQALAQPI
                     ASATDAGIPVIVIDSNANTDKYVTFLATNNVTGGEKAADELAACVKRRTGKEEGDVAY
                     MTALAGAQSLNDRDKGFVQEMKKYPGLKIVEHRTGNNDPARALSDAEDILTRHPNLVG
                     LFADNELEGDGAGRAIQEKSLGPKMCAVAFDTSDQEVAFVRNGNLDGLIVQNPFMMGY
                     AGVWYALAAANGVVLPHYVDSGVHVVTKQNIDSPKMAGLLDPKKYQLSPFLGG"
     gene            14903..15889
                     /locus_tag="VKU03_13445"
     CDS             14903..15889
                     /locus_tag="VKU03_13445"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72319.1"
                     /translation="MASADTHAETSASGGELRLGLPVETWVVGCTALLWIGLAFASPY
                     FLTSGNIFNMMRQVSVDAIVAYGELFTIITAGIDLSVGAVAGFTGVAFAIFLAGGMNM
                     ALAAVLTLLIGAAIGLINGLAVNRLGIPAFIVTLAGLQGYRGLTMLISGGMTVAGLPD
                     SLGLVANTSILGVPTMFLIMAIFAIGSQYLLGVTRLGRYMYALGSNIEAARRVGVNVT
                     RVTLAAYGLASIFATVGGMLLVARLTMGNPNAGSGAELQAIAAAVVGGASLFGGRGTI
                     GGCFIGAVLFTTIGNGANLLGVNSFWQMVIEGLLIAGVVYLDNVQKRRGQGQ"
     gene            complement(15948..>16432)
                     /locus_tag="VKU03_13450"
     CDS             complement(15948..>16432)
                     /locus_tag="VKU03_13450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012453588.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="transcription termination factor Rho"
                     /protein_id="HLJ72320.1"
                     /translation="DVVILLDSITRLGRAYNTVVPSSGKVLTGGVDANALQRPKRFFG
                     AARNIEEGGSLTIIATALIDTGSRMDEVIFEEFKGTGNSEIILDRKVADKRTFPSMDI
                     TRSGTRKEELLVPADILKKMYVLRRILNPMGTVDAIEFLIGKLRETKNNAAFFESMNT
                     "
BASE COUNT         2660 a         5670 c         5293 g         2809 t
ORIGIN      
        1 tacagcgtga tcaatccgag gatccgccat gctcggtagg ttgacgtatc ctcccctgcg
       61 aagcggggga gggggaccgc ccgaagggcg gtggaggggg ccgccggagg ctgggtttta
      121 tcctgagaga ccggagcccc ctccaccatg cttcgcatgg tccccctccc ccgcttcgcg
      181 ggggaggatt gacggcaatc gccgatgacc gccgtccccg cgcccgtcgc ttccgccgag
      241 gcgcgcgagc gcgcggccga acttgccgcc ttctgggccg tctatcgcga gaacaaaggc
      301 gcggtgttcg gcctttgcgt gcttgcgctc atcgtccttt gcgcgatttt cgccaatgtc
      361 atcgcgccct attcgccgat cgaacaattt cgcgacgcga tccgcgcgcc gccggtttgg
      421 gacggcggca catggagatt cgcccttggc gccgacgggc ttggccgcga cattctctcg
      481 cggctgatct atggcgcgcg catttcgctg ttcatcggcc tcgccgtcat ggggctctca
      541 tcggcgctcg gcgtcgcgct cggcctctcg gccgcctatt tcggcggcct gatcgatgtc
      601 gccgtctcgc gtctcaccga cgtgatctgg gccttgccga gcctggtcct ggccgtactg
      661 gtcatcgcga ttatcgggcc gagcctgttc aacacgatca tcgccatcgc gatcgtctat
      721 acgccgactt tcgtacggct gatgcgcgcc tcggccctcg cggaattgtc caaggactat
      781 gtcgtcgccg cccgcgccgt cggcgtcggg ccgctgcgcc taatgctggc gaccgtcctg
      841 cccaattgcc tcgcccccta tatcgtgcag gcggcgatcg gcgtctccag cgccattctc
      901 gaagcggcgg gcctcggctt tctcggcctc ggcgcgcagc cgccaacgcc ggaatggggc
      961 gcgatgctcg ccgacgcgcg cgaatttatc cgctccgatc cctggaccgt caccctgccc
     1021 ggcctcgcca tcgtggtcac ggtgatcgcg atcaacctga ccggcgacgg cctgcgcgac
     1081 gcgctcgatc cgaaaatgcg gcggagctga gcatggcgct gctcgacatc cgcaatctct
     1141 ccatcgcctt cgccacccgc cacggcccgt tcaaagtggt cgacggaatc gagctttccg
     1201 tggagcgcaa cgaggtcctc gccattgtcg gcgaatcggg ctccggcaag tccgtcgcca
     1261 tgctcgcggt gatgggcctg ctgccgcata ccgccagcgt gaccgccgac cgcctcgcct
     1321 tcgaaggccg cgatttgcgg acgctgtcgc cgaaggagcg gcgcgcgatc atcggcaagg
     1381 atattgcgat gatcttccag gagccgatgt cctcgctcaa tccctgcttc accgccggct
     1441 tccagatcgg cgaggtcctg cgcacgcatc tcggcatgaa tcgcgcggcg cgcaagcggc
     1501 gcgtcttcga attgctcgaa gaggtcggca tcgccgatcc ggagcggcgc gcgcgggcct
     1561 tcccgcacca gctttccggc ggcatgagcc agcgggtgat gatcgcaatg gctttggcct
     1621 gccggcccaa gctgctgatc gccgacgagc cgaccaccgc gctcgacgtg accatccagg
     1681 cgcagatcct tgacctgctc ttaaagctgc gccgccagaa cggaatgggg ctcgtcctca
     1741 tcacgcacga tatcggcgtc gtcgccgaaa cggccgaccg ggtcgccgtg caatatgccg
     1801 ggctgcaggt cgagacaaat acggcgcgcg ctttgttcgc cgatccgcat catccttacg
     1861 ccgccgccct gctcgccgct ctgccggagc gggccaacgg ccggcggctg ccgtcgattc
     1921 cgggcgtcgt gccgggccaa ttcgacaggc cgagcgcttg cgtcttctcg ccgcgctgca
     1981 gcttcgcgtt cgagcgttgc cgccgcgaag cgccacggcc ggccagcgcg gcgctcgggc
     2041 gggcgctgtg ccatacgccg ctcatcgggg gcgtatcgtg accgccgccc tccaagcgcg
     2101 cggcctcagt cgcgattatg cgctctcgcg cggtctcttc acgccgcgca tgacggtgaa
     2161 ggcgctggtc gacgcctcct tcgatctcga tgccggcaag acgctcgccg tgatcggcga
     2221 atcgggctcc ggcaagtcga cgctggcgcg gctcctcacg cttctcgaac aaccgacggc
     2281 gggagcgcta accatcgacg gcgaggacgc gatcggcgcc ggcgcggaaa cccgcaagcg
     2341 gctgcgccgg caaatccaga tcgtcttcca gaatccctac ggctcgctca atccgcgcca
     2401 gcgcatcggc aaggcgttgg aggagccgct gctcgtcaac accaatctct cggccgccga
     2461 gcgcgagaac gcggcgcgcg ccatgatgga gaaagtcggc ctcaggccgg aatattatcg
     2521 ccgctatccg cacatgtttt ccggcggcca gcgccagcgc atcgccattg cccgggcgct
     2581 gatgctgaga cccaaaatcc tggtcctcga cgagccggtc tcggccctcg atgtctcgat
     2641 ccgcgcccag acgctcaatc ttctcgccgc tttgcaggag gagtatcgtc tcgcctatgt
     2701 cttcatctca cacgacctct cggtcgtgcg ccacatcgcg gacgaggtga tggtcgtcta
     2761 tctcggccat gtcgtcgaaa agggcgcgcg cgcggcgatc ttctcggcgc cgcagcaccc
     2821 ctatacgcgg gcgctcctct cggcgacgcc ggtcgccgac cccgccgccc agcgcgagcg
     2881 gatcattctc tcgggagaat tgccgtcgcc gttccatccg ccttccggct gcccgttcca
     2941 tcctcgctgt ccagcggcga tggatcgctg ccgcgccgag aacccggcgc tcgagcaaaa
     3001 gcagggccgc gacgtcgcct gctgggcggt ggcggcatga ggagtggcgc tgaaggcacg
     3061 tatccttccc cgcgaagcga gggaggggga ccgcccgaag ggcggtggag ggggccgcca
     3121 gcggttggga tttatcctga gagaccgaag ccccctccac catgcttcgc atggtccccc
     3181 tcccccgctc cgcgggggag gaaaggctgc gccgcgccat gaacgacatc cgtaccttcg
     3241 actatatcat cgtcggcgca gggacggcgg ggtgcttgct cgccaatcgc ctttccgccg
     3301 acccacgcaa tagagtcctg ctgattgaag caggcgggcg cgacaattgg atctggttcc
     3361 acatcccggt cggctatctc ttcgcgatcg gcgataagcg cgccgattgg ctgttcaaga
     3421 ccgcggccga gcccggcctc aacggcagag cgatcgccta tccgcgcggc aaggtgatcg
     3481 gcggctgctc ggcgatcaac ggcatgatct atatgcgcgg ccaggccgcc gattacgacg
     3541 gctggcgcca gctcggcctt gagggctggg gttgggacga cgttctgccc tatttcaaga
     3601 agcacgagga ttatcacggc cgcgccgacg ccctgcacgg cgtcggcggc gaatggcgca
     3661 tcgaacgcca gcgcctgcat tggccgatcc tcgacgccgt ccgcgaagcc gcggcggaga
     3721 tcggcatccg caagaccaat gacttcaatt ccggcgacaa cgaaggctcc gactatttcg
     3781 acgttaatca aaagcgcggc ctacgctgga gcgccgcgcg cggctttttg aagccgattc
     3841 tccatcggca gaatctgacg ctcgtcaccc atgcgcaggt cgggcagatc ctctttgcgg
     3901 acaagcgcgc cgtcgggctg cgctataagg gcgaaggcgc gagcatagaa gcccgcgccg
     3961 cgggcgaaat catcctcgcc gccggcgcga tcggcacgcc gcatctgctc gaattgtcag
     4021 ggatcggccg gccggaaatc ctagcgcgag tcggcgcgcc gctcgtccat gcgctgccgg
     4081 gcgtcggcga gaacctgcag gaccatctcc agcttcgcac gattttcaag gtctccggcg
     4141 cgcgcacgct gaacatggat tacaaatcgc tcgtccggcg cgccctgatg ggcctcgact
     4201 acgccttccg gcgccgcgga ccgctgacca tggcgccctc gcagctcggc attttcgcca
     4261 agacctcgcc cgattacgcc accgccaacg tcgaatggca tgtccagccg ctctcgctcg
     4321 accgcttcgg cgagccgctg cataggtttc cggcgatcac gctcagcgtc tgcaatctca
     4381 ggccggcgag ccgcggcagc gttcacgccg cgagcgccga ctcgtcggcg gcgcccgaga
     4441 tcaagccgaa ctatctttcc gaggaggagg accgccgcgt cgcggtcgac gcgatcaagc
     4501 tgacgcgccg catcgccgcc gcgccggccc tcgcgtctta taagccggaa gagctgctgc
     4561 cgggcgcgtc gaaggcgagc gacgaagacc tcgccaaagc ggccggcgag atcggcacga
     4621 cgatctttca tcccgtcggc acggcgaaaa tgggtacgca agacgaccca gccgccgtgg
     4681 tcgatgcgca gcttaaggtg cgaggcctcc gcggcttgcg catcgccgat gcttcggtca
     4741 tgccgcgcat cacgtcgggc aacaccaatt cgccggttct gatgatcgcg gaaaaagcgg
     4801 cggcgatgat tctcgaagag gccaagcggt gagcgtcgaa ggcttcgccg agccgccggc
     4861 ttctccctat gagccgggcc ttgccgacgg ccgccagtcc gagacggctt tgttcatcgc
     4921 gcgcggcttg cggcgcatgc tgcgcgccca tggctattca agcgtgacgg aattgccgct
     4981 gctcgatggc cgccgcgccg acgtcgtcgc gctgcgcggg gatggatcgc tggcgatcat
     5041 cgagatcaaa tcctcggtcg ccgatttccg cgccgacggc aaatggcgcg actatctcga
     5101 ccattgcgac cggctctact tcgcgatttg cgacacgatc ccgcccgaga tcatgccgcg
     5161 cgaagcgggc ctgatcatcg ccgacgccta tggcgcggcg atcgttcgcg aagcgccgga
     5221 gcagaggctg gcgccggcga cgcggcgcgc cgtgttgctg cgcttcgccc aggccgccgc
     5281 ggacaggctg catcggctcg ccgatccggg cggattggcc gacaggtaag tacaagaccg
     5341 tcattgcgcg cgaagcgacg caatccattc gtaaagcgcg cccgatgagc ggactgtttt
     5401 gtctcctcgc tacgctccgc tccccgcgat ggcggcggca tcagcggcgg cgatgctgca
     5461 acaccatcgg catgccgcct ttcggccgga gcgtgacctg ctggaccggc gccactttgt
     5521 gccccggcgc gagatcgaga cggtacgaac gcagaatcgc ggcgagaaaa atcgtcgcct
     5581 cctgcagcgc gaaaggcatg ccgatgcaag agcgcgcgcc cgcgccgaac ggcagatagg
     5641 cgaaccggtc gatcttctcg cgctcgccgg gcatgaagcg cgtcggatcg aaatggtcgg
     5701 gatcgcgcca cagcaatttg tgccgatgca ccacataggg cgagatcacg atcagcgtgc
     5761 cgggatggat tttctgtccg gccagcatgt cggcggccaa cgcttcgcgg ctcagcgacg
     5821 gcgccggggg gtaaagtcgc atcgcctcgt cgagcgtcgc gcgcacccgc ggcaagcgct
     5881 cgatcgcctc aacatcgagc gggcgctcgc cgagcgcgtc gtcgacttcg cgctcgacct
     5941 cttcgcgcca ggcgggatgc tggctcaaca ggaacagcga ccacgtcaag gcgttggcgg
     6001 tggtctcgtg cccggcgccg atgaaggtga cgacattggc cctgacctcg acctcgctga
     6061 ggccctcgcc ggtttgcgga tcgcgcgcgt cgagcagcat ggtcaggaga tcgcgcggcg
     6121 agccttgttc ggcgggcggc ggcgcgccat gggcgcggcg cgccgcgaga atatcgtcca
     6181 ccgcggcgtc gaagaatttc agcgccttga ccgggctgcg ctttccgacg cgcggtagcc
     6241 attccggcgc gtcgagcaga tcgaaaggat gcagcgcgcc cagcgccgtg acgagctccg
     6301 acatgccgcg cgcgaaatcg cgcggctcgc ggctgaggcc ggaaggaaaa atcgtacgct
     6361 ggagcgcttc aagcgtcgcg accgacattt cgccggctat gtcgagcctt cggcccggcc
     6421 gcagcagcgc ccagcgctcg atcagccatt cggcggtcgc gaccatcgca ggcgcgaagc
     6481 tcaacaccat gcgcggcgtg aacagcggcg ccagcgcgcg gcgctgcttg cgccagtcgc
     6541 cgccctcgct cgtcagcagc ccgtcgccga ggccaggcga gagcacgcgc agttgcagcg
     6601 ggtccttcgg gtagtttgcg gcgttgtcga tcagcacgcg cttgatcgcc gccggctcgc
     6661 agaccacggc gatgcggccg agcaggccat tggcctgggt gatcggccat tcgaaattga
     6721 agcgcgtcca agtgtcgagc ggattgcgcc agaagcggat caggagcgac agcgggccaa
     6781 gcggccgcgc attcggtatc ggcgccggcg gccgcgggcc gaggggcgac ggcttggcga
     6841 ggtcgctgac gctcatggcg ggctccttcg gctgatcgcg cgctttcaac gtatattttg
     6901 ccgccatggc gcgcagcaaa gagcgccggc gctgaattta tgaaacccgc cggacaggag
     6961 gctcgacttg agggcaaact cattataacg tcataaactc agttcgggag gaggaaccta
     7021 atgaacatgc gtttcggctt atcgatgacg gctcttgccg taaccatgct cgccagcgcc
     7081 ggcatggcca gcgccgaccc ggtcgccatc tctttcctca cccattggcc gccggagacc
     7141 gtcgccaagc tcaaggcggc gacggacgcc tattccaagg ctcatccgga cgtgaagatc
     7201 gacatccgag cggtgccgtt tggcgatctc ctgacgacga tccgctcgca aggggcgagc
     7261 gccaacggcc cgacgatcga cggcatttac gatctctggc tgcccgaact ggtgcgcgac
     7321 aagctggccg cgccggcgcc agccgatctg gcgacggatg tcgccaaaaa ctggccgaag
     7381 ggcgttgcga ccgcggccag catcggcggc gcgctctatg gaatccccaa cgagatcgac
     7441 ctctacacgc tcaattacaa caagaaactg ttcgccgagg cgggcgtcgc cgggccgccc
     7501 aagacctggg ccgagctcga agccgacgcc gagaagctca ccaaaaagag cggcgggacg
     7561 acgacgcagc agggcttcgg cctgatcaat tcctgggccg ccggcgtggt ccaccccttc
     7621 gcctcgctgc tcgcctcgaa cggcggcgcg ctgatcgtcg acggcaagcc cgcgctcgat
     7681 tccaagcagg ctggcgagac tttcgcgctt tacgagaagc tgatcaaatc cggcgcgagc
     7741 gttcccgcca tgggcacggc cgacgccaac acgaccgggc cgttcctcga caatttcgtc
     7801 tccggcaaga cggcgatgat catcatggcc aattggtggg agagcgcgct gaaagcgggg
     7861 atgaaggaca atttcaagga catcgccgcc gcgccgatcc cggtcgggcc gagcggcgac
     7921 gccccgcact cgatttcgta ctcgtggatg acggtggtca acgccaaggc ggagcccgcc
     7981 gcacaaaagg cggcctggga cttcctggcc tggctcaacg gtccgacctc cgggccgcac
     8041 ggcgcctcgg ccatgggcga catcctgatg tcgatgggca tccttccgtc gcgaacctcc
     8101 gacgtcgcgg cgttcaagga tcaactgagc tcgccgttcc tctccgccta tgtcgccgcc
     8161 ttgccggaag cgcggccttt ccccgacgtg ctcggcggcc aggaattcac cgaagccctg
     8221 cagcagcaca tcgaggcgat cgagttcggc aaggaaagcg ccgccgcggc gcagaaagcg
     8281 gcgcaggagg acgccgtctc gatcctgacc cgagcggcga agtaggggtc ttcccctcgc
     8341 cccgcgaagc ggagagggtg gcccgagggg ccgggtcggc agcgcggcgc ttggaactag
     8401 cgagtgatga tttgaggctg aacacttgca ccgtcattgc gaggagcgta gcgaccaagc
     8461 aatccaggag ccaaggcggt tcctggagtg cttcgcttcg cttgcaatga cggagcggct
     8521 tggcctaaga gcggcgcgtg gcggctctcg gaaggcgcat aaccttcgag tcctgcgcag
     8581 cccctcatcc ggcggcttcg ccgccacctt ctccccgcga agcggggaga agggatgtcg
     8641 cgcgtcatga tccgcagcgg cgcattcctc ctgcctcgca tcgcgctgct cgcgccgggc
     8701 gtcggcctga tcgcggcgtt catgttcgcg ccgatcctgc tgacggtctg gctctccttt
     8761 cacgcttggt cgacgcagac gccgttcagc gcggcgcgct ttgtcggcct cgccaatttc
     8821 gccgatttgt tcgggccgac atccgtcggc cgcgatttcc gcggcgcttt cgccaatacg
     8881 gcgctctacg ccatcctctc gatcgcgctg atcctgccgc tctcggcagc gctcggcgtg
     8941 ctcatccacc agagccgcgc gatcggccgc aacgcgctgc gcgccgtcct gttttcgacc
     9001 tatatggtcc cgtcgatcgc cgtcgccctc gtctggtcga agctctattc gccgaccgaa
     9061 gggccatacg atcaaatgct cgcctggctc ggcctgccga cctccgactg gctctcctcg
     9121 ccgaacaccg cgctggtttc gctcgtcctg ctcaacgtct ggcagcagac cggctatttc
     9181 accgtgctgg cggtcgccgg cctcacgcaa attccgacga cggtttacga agccgcgacg
     9241 cttgacggcg ccaatcgctg gcaggccttc ggccatgtca ctctgccgct gttgcggcgg
     9301 acgctgctgt tttcgagcgt catcgcgctg atcaacgccg tgcaggtctt cgagccggtg
     9361 gcgctgatca cgcaaggcgg cccggtcggc gcgaccaacg tcctgacgta ccatatccgc
     9421 cgcgtcggcg tcgagcgggc gcaaggcggc ctcggctcgg ccatggcggt gatgctgctt
     9481 ctcaccctga tcgccacggt cgccgtcctc ttcgccgccg cgcgcgagag gcgcgggcca
     9541 tgagcggcgc ggcggtttcg acggcgaccc gtccgatcgc gctcccccgg ccgcggcggc
     9601 ggcgcgacgc gccgactcag atcgcgcttg ccggcctcgt catcgccatc ggcctcgctg
     9661 ccgcctttcc gctcgcctgg atggcgctga gcagcctgaa aacgccgtcc gagacgatgc
     9721 agacgccgcc ggtctggtgg ccggcgacgc cgtcgctcga cgcctactcc aaggtcgcca
     9781 acgtcatcga ggcgggccgc tcgttcctga actctgcgat catcgcctcg gcggcgacgc
     9841 tcggcattct cgtcaccagc ctgatggccg gctacgcctt cgccaagcac caattcaggg
     9901 gccgcgacgc gctgttcgcc gtgatcgtcg cgaccatgtt cctgccgccg atcgtcacct
     9961 tgatcccgct ctaccgcatg gtcggctcgc tcggcctcga cggcagcctt ctcggcgtga
    10021 tcctgcccaa tctcgccaac gccttcggca ttttcctgat gcggcaattc atcgccggcg
    10081 tcccgaatga gctgatcgag gcggcgcgga tcgacggcgc ctcggagctg cgcatcgtct
    10141 tcggcatcgt cgcgccgaat gtcctgccgg cgatcgcgac gctcgcgatc ttcgccttct
    10201 gctaccattg gaacagctat ctctggccgc ttaccatcct gcagggcaat ggcgacgcct
    10261 atccgatcgt gctcagcctc agccggctgc tcagctacaa tcgcggcgcg atgaacacca
    10321 atctggtgat ggcgggggcg acgctcgccg tgctgccgcc gctgcttctg ttcatcgccc
    10381 tgcagcgcgc cttcatcagc agcatcatcc aatcggggct caacggatga gccgcgtcct
    10441 cgccctcgat ctcggcggca cgcatctgcg cgccggactc gcagccgcca acgaggccgc
    10501 ctccgtgcaa acgatcggcc gctggccggc gccgcgcgac atggaatcgt tccgcgtcct
    10561 gctcggcgag atcgtggcga gccgcgggcc gttcgaccgg gtcgggctcg ccgtccccgg
    10621 cctcgccgag gaaacgcgct gccgctggat acccaacctg ccctggctcg acggcgtcga
    10681 cctcgccgat ctcgtcgccg gggcgccgct ggcgctgggc aacgattcgc aattggcgct
    10741 gctcgccgag gcgacgcatg gcgcggcggc cggcttgagc gacgcgatcc ttttgtcgat
    10801 cggcaccgga atcggctcgg cggtcctttc cggcgggcgc atcgtcaaag gcgccgcggg
    10861 cggcgcctgc tcgttcggct gggcctgcgc ggatatggcc gatcaaggcc gccacaagga
    10921 cggctggctg gagcgcatgg ccgccggccg cgctttcgac gcggccgccg tcaagacctt
    10981 tggcgcggcg gacggagcgg ctctgatcgc cgcggcccgc gccggcgatc aagcggcgat
    11041 cgaggccatc gccaaaccgg ccgcggcgat cgggacggcg ctggccggcg ccgtcgcgct
    11101 gctcgatccg caggcgattc tcttcacagg cggcgtcgcc gaatcgctcg acgcgctagc
    11161 gccggccatc ctcgctgctt tgcgccgcca tttgccgccg catctgaagc gcgtcgcgtt
    11221 gcgggcggga cgattttccg gcagcgccgc gctgatcggc gcggcaatcg ccggcgcggc
    11281 tggccgcgat tggcggagcc tcagatgacc gcgatttttc cattgcccga ccgccgccgc
    11341 cccgagccgc tctggcatca atgcgagcgc tcgatccaag cgctgatcga aagcggcgaa
    11401 tgggcggccg gcgcgcaaat cccggccgag gaccggctct gcgccatgct cggcgtcagc
    11461 cgcatcacca tccgtcacgc tctgcgcaat ctcgaagaga gcgggctgct gcggcgcgag
    11521 catggccgcg gcacgttcgt gcgcagcgcc acgctgatcg ccggcgcgcg cggcctgacg
    11581 agctttaccg aggaaatgtc cagccttggc cttgccgccg gctcgcgcct gctcgatcag
    11641 gaggtgatcg cggcctccgc cgacatcgct gcggcgctcg acatcgaaga aggcgcttcg
    11701 gtgctgcgta tccgccgctt acggctcggc ggcgatgcgc caatcggcgt ccagaccgcg
    11761 cacttgccgc tcgaccgcgt cgcggcgctg cttggcgccg gtcccctcga cggctcgctc
    11821 tatgcggcgc tcgaaacgcg ctgcgggatc aagccggaag aagcgcgcga gacctatcgc
    11881 gtcgcgctga tcgacggggc cgacgcggcg ctgctcggcg tcgcgcccgg cagcccggtt
    11941 ttcgtcgtcg agcgcatcac cgccgacggc cgcggcgcgt tcgaattcac cctctccctg
    12001 atgcgcggcg atcgctacga gatccgctcg accctgcgcg ccaagccctt ttcacaggag
    12061 cgactatgac cgacctctat atcagccgcc tgaccgatct catcgagaag gcgagcaaga
    12121 ccaacgccgc gaccttcgac agcgtcgcca agcggctcgc cgagaccttg cagaatggcg
    12181 gcctgatcca tctctacggc tccggccatt ccgtcctgcc ggtccaggaa gcctttccgc
    12241 gctacggctc ctatgtcggc ttcaacccgc tgaccgatcc gcgggtaatg tggcacaacg
    12301 tgctgggcgc cggcggcgtg cgcgaattgc tgtggctgga gcggaccgag aaatacgcag
    12361 agaaattcct cgaccaccag ccgctcaacg ccggcgactg catcatcatt tacggccata
    12421 gcggccgcaa cgcgtccggc atcgatgcgg cgctctatgc gaaaaaacgc ggcctgttcg
    12481 tcgtcgcgat caccgccaag gccaatctcg acaagccggc gacgcattcg tccggcaaga
    12541 ggctcgccga cgccgccgac gcggtgatcg acacctgcgc gccgatcgag gacgcgatcg
    12601 tgccgattga aggctggagc cggccggtct ccggctcctc gacggttctc gccatgatca
    12661 tgacgcatga gttgatcgcg agaacggcgc aggcgttggc gaagcgcggc gtcgaactgc
    12721 cgaccttcgc ctcgccgacc atagccggcg tcaccctgca cgacacagat gtcatttacg
    12781 gggtgtatcg cgaccgcctg atcgaggcgc aaagaaagca cctgccggcg tttcaagcga
    12841 agatgcgtgg ggaaggctag ccggcgtccg gcgctcaatc gtaccttatt cattcgcccg
    12901 tttgcgggcc ggcgtgcgac cgattggcgc gggccataag ccgcggaatc aggaacttca
    12961 cacgcgccgg ccggttgatt tgggcagggc aggcggggca gggaggacga cgtgccgccg
    13021 ctgctcgaag tggcagacct ccataagagc ttcggaccga tcaatgtcct gcgcggcgtc
    13081 gatctgtcgc tcaatgccgg cgaggtgttg gcgctggtcg gcgacaacgg cgccggtaaa
    13141 tccaccctga tcaagctgat ttcgggcgtg cacaagccgg attccggaga aatccggttg
    13201 aacggcgcgc cgctcgacgt gcatggcccc gccgatgcgc ggcgcctcgg catcgagacg
    13261 gtctatcaag accttgcgct cgccgacgac ctctccgtcg gcgccaatgt ctttctcggc
    13321 cgcgagccgg tcaggcattg gctcggcctg ctgccggcga tcgacaaccg caagatccgg
    13381 atcgagaccg actcgctctt aaagcgcatc gaaagccgca tcccgcccaa tgcgcgcacc
    13441 gtctcgcgcc tttccggcgg gcagcgccaa gcggtggcca ttgcccgcgc gctctattgg
    13501 caatcgaaag tcgtcattct cgacgagccg acagccgcgc tcgcggtgat ggagcgcgag
    13561 catgtcatcc aactcgccag gcggatcgcc gaggagggga tcggcgtcat ctatatcggc
    13621 cacaatctca tcgagattct cgaagtcgcc gatcgcgtcg ccgtgatgtt ccaaggccgc
    13681 atcgttcacg tcaccccggt cgccgagacc agccagcgcg atttgatcaa atacatgacc
    13741 ggcttcaccg aatccaagga ggcgctgtct cacgaggggc gaacccaagg aaggagataa
    13801 acatgttgaa gcttacgctc ttagccgcga cggccctcgc gctcgcggcc ggtccggcgg
    13861 ccgccgccga tttcagcaag gaccgcattc cggtgatcgt caaggcgacg acgtcgcaat
    13921 attgggcgac ggtgttcgac ggcgccgacg ccgcggccaa ggcgctcggc gtgcagatct
    13981 ccaagctcgg cgccagcgcc gaaaccgatg tggcgcagga agtctcgatc atggagaacg
    14041 ccatccagtc gaagccgacc gcgatcgtca tcgccgcgac caacgcccag gctctcgccc
    14101 agccgatcgc cagcgcgacc gacgccggca ttccggtgat cgtcatcgat tccaacgcca
    14161 acaccgacaa atacgtgacc ttcctcgcca ccaacaacgt gaccggcggc gagaaggcgg
    14221 cggacgagct tgccgcctgc gtgaagcgac gtacagggaa agaggaaggc gacgtcgcct
    14281 acatgaccgc gcttgccggc gcgcaatccc tgaacgaccg cgacaagggc ttcgtccagg
    14341 agatgaagaa atatccaggc cttaaaatcg tcgagcatcg caccggcaac aacgatccgg
    14401 cgcgcgccct ctccgacgcc gaggacattc tgacccggca tcccaatctc gtcggcctgt
    14461 tcgccgacaa cgagctcgaa ggcgacggcg ccggccgcgc catccaggag aagagtctcg
    14521 gtccgaaaat gtgcgccgtg gcgttcgaca cgtccgatca ggaggtcgct ttcgtgcgca
    14581 acggcaatct cgacgggctg atcgtccaga atcccttcat gatgggctat gccggcgtct
    14641 ggtacgcgct cgccgcggcg aacggcgtcg tcctgccgca ttacgtcgat tccggcgtcc
    14701 acgtcgtcac caaacagaat atcgacagtc cgaaaatggc cggattgctg gacccgaaaa
    14761 agtatcagct ttcgccgttt ctcggcggat gaggaatagc ccgtcattgc gaggagcgaa
    14821 gcggcgaagc aatcccctca tagggcggcg ctctatgaac ggattgcttc gctcataatg
    14881 acggccaaac gggacgagaa tcatggcgag cgccgatacg catgccgaga caagcgcctc
    14941 cggcggcgag ttgcgcctcg gcctgccggt cgaaacctgg gtcgtcggct gcacggcgct
    15001 gctgtggatc gggcttgctt tcgcctcacc ctattttctg acctccggca acattttcaa
    15061 catgatgcgc caggtgtcgg tcgacgcgat cgtcgcctat ggcgagttgt tcacgatcat
    15121 caccgccggc atcgacctct cggtcggcgc ggtggcgggc tttaccggcg tcgccttcgc
    15181 gatcttcttg gccggcggca tgaacatggc cctcgctgcg gtgctgacct tattgatcgg
    15241 ggcggcgatc ggcttgatca acggcctcgc cgtcaaccgg ctgggcattc cggccttcat
    15301 cgtcaccctc gccggcttgc agggctatcg cggcctgacc atgctgattt caggcggcat
    15361 gacggtggcg gggctgccgg attcgctcgg cctcgtcgcc aacacgtcga tcctcggcgt
    15421 gccgaccatg ttcctgatca tggcgatttt cgcgatcggc tcgcaatatc tgctcggcgt
    15481 gacgcggctc ggccgctaca tgtacgcgct cggcagcaat atcgaggcgg cgcgccgcgt
    15541 cggcgtcaat gtcacgcggg tgacgctggc cgcctatggc ctcgcctcga tcttcgccac
    15601 agtcggcggc atgctcctcg tcgcgcgcct gacgatgggc aatccgaacg ccggctcggg
    15661 cgccgaattg caggcgatcg ccgccgccgt ggtcggcggc gccagcctgt tcggcggccg
    15721 cggcacgatc ggcggctgct tcatcggcgc ggtcctgttc accacgatcg gcaacggcgc
    15781 caatctgctc ggcgtcaatt ccttctggca gatggtgatc gaaggcctgc tgatcgccgg
    15841 cgtcgtctat ctggacaatg tgcagaaacg gcgcgggcag gggcagtgat ggggctggcg
    15901 tccgcgttgg gacggaatgc tttcagcgcc gcgcccgcca cgccgcgcta cgtgttcatc
    15961 gattcgaaga aagcggcgtt gttctttgtc tcgcgcagct tgccaatcag gaactcgatc
    16021 gcgtccaccg tgcccatcgg attgaggatg cggcgcagca cgtacatctt cttcagaatg
    16081 tcggccggga cgagcaattc ctccttgcgg gtgccggagc gggtgatgtc catcgacggg
    16141 aaggtgcgct tgtcggcgac tttgcggtcg agaatgattt ccgaattgcc ggtgcctttg
    16201 aattcttcga aaatcacctc gtccatgcgc gagccggtgt cgatcaaggc ggtcgcgata
    16261 atggtcagcg aaccgccttc ctcgatattg cgcgccgcgc cgaaaaagcg cttcggccgc
    16321 tgcaaggcgt tggcgtcgac gccgccggtc agcaccttgc cggaagaggg cacgaccgtg
    16381 ttgtaggcgc ggccgaggcg ggtgatcgaa tccaagagga tgaccacgtc gc
//
LOCUS       DATGJW010000447         3552 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1436386, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000447 DATGJW010000000
VERSION     DATGJW010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3552)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3552)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3552
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1436386"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(1..558)
                     /gene="cobI"
                     /locus_tag="VKU03_13455"
                     /pseudo
     CDS             complement(1..558)
                     /gene="cobI"
                     /locus_tag="VKU03_13455"
                     /inference="COORDINATES: protein motif:HMM:NF012799.4"
                     /note="internal stop; incomplete; missing C-terminus;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
     gene            complement(555..1184)
                     /locus_tag="VKU03_13460"
     CDS             complement(555..1184)
                     /locus_tag="VKU03_13460"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020094634.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HLJ72321.1"
                     /translation="MKPAYAYEKDPAAIYRRSFAIIRGEADLQRFSALEERVAVRVIH
                     ACGMVETARDMVFSPHAAEEARAALRGGAPIFCDARMVAEGITRARLPRDNDVICTLG
                     DPSVPALAAKLGTTRTAAAIDLWLPRLGGSLVAIGNAPTALFRLIEAIAAGAPRPAAI
                     VGMPVGFVGAAESKEALLESGLPAIVVRWRKGGSALAAAAVNALASDTE"
     gene            complement(1181..2491)
                     /gene="cobG"
                     /locus_tag="VKU03_13465"
     CDS             complement(1181..2491)
                     /gene="cobG"
                     /locus_tag="VKU03_13465"
                     /EC_number="1.14.13.83"
                     /inference="COORDINATES: protein motif:HMM:TIGR02435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0019250 - aerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HLJ72322.1"
                     /translation="MSAIAARRPPPPYRKGWCPGALRPMETGDGLLARVRAPQARLSL
                     DQALALAAAAMACGNGLMVLSARANLHLRGLSEPTLPELHARLGDAGLIDADPEIERL
                     RNIIISPVDDLDPDAEFDLGVSVSALETRLREDERLRQLPAKFGIVLDAKGRLPLDDV
                     DADIRFEAQRDGTFAVFLGGEDALCGECAPVQTGEIAARLGLSFMALAGAGEAAPRRM
                     RALIEETGAAAVFAEAACEAKPGARSKRGASLSDVLGGHAYGGLVVVGAAAAFGEFEA
                     VRFKALIERARALGATGLRLTPWRAFMIAGLEPRAAAAMEKACAKLGFICDSGDQRLR
                     VAACPGAPACMHGARPARDDAAQWAVLLPNGEGVVLHVSGCAKGCARPAATAATLTAT
                     AAGYDLIVAGRAGDPPARRGLKSAEVAALLAGEGTLMFGPRGRP"
     gene            complement(2488..>3552)
                     /locus_tag="VKU03_13470"
     CDS             complement(2488..>3552)
                     /locus_tag="VKU03_13470"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HLJ72323.1"
                     /translation="GRRAAQEILQRYAQDHGEWPKRIVLDLWGSATMRTGGDDLAQAF
                     ALLVCRPTWDVASTRVSGFEVLPLAMLGRPRVDVTLRISGLFRDVFPSQIALFAAAVR
                     AVAALEESAGDNPLASLREPARIFGAAPGAYGVGLGARIARGDWAGRSELAEAYLAAT
                     SHAYGGEGEAREAAAEFRQRVAGAEAFVHVQDLPGQDALDADAFAEHEGGFAAAAAEA
                     GATPALYHLDSTVAHAPKIRTLKQEIARALRGRAANPRWISGQMRHGHRGAAEIAQSL
                     DNLYCFAALTDAVESTQFDLMFDATLGDDSVRAFLLQANRRAASHMAGVFEEAARRGF
                     WRSRRNSSARILSSVLTEAA"
BASE COUNT          570 a         1251 c         1250 g          481 t
ORIGIN      
        1 gcggccgatc ttcatgatca ccgcggcgtc ggccagcctg aggcgccgag tgagctcatc
       61 gagcgtaagc gtcgcgggga ggacaacaag cgcatcgtcg ccccaggtca tcggcatggc
      121 cgccgcgccc caggcgccgc agatgctgga aacgccgggc acgatcgcga cctcgaaatt
      181 gtcgcgcaaa cggacgaaca gatgcatgaa cgagccgtag agcatcgggt cgccttcggc
      241 gagcagcgcg acgtccctgc cggcctcgag atgggcggcg atcgcgaccg tgctctcttt
      301 ttagaagcgc gccagcgcct cgcggtaggg cggatcgtcg aaatgcgatt cgtcagtcat
      361 cggataatag agcggcagct cgacatgcga gggcttgagc cagcagtcga cgatcgcgcg
      421 cgcgttgccg cgccgtcccg ccttggcaaa gaaggcgacg acgggggcca aggcgatgac
      481 ccgcgtcgcc ttgacggtga gcaattcggg atcgcccggg ccgatcccga cgccatagag
      541 acggccgaag gcggtcattc ggtatcactc gccagcgcat tgactgcggc ggccgcaaga
      601 gcgctgccgc ctttgcgcca gcgcacgaca attgccggga ggccgctttc aagcaacgcc
      661 tccttcgatt ccgcggcgcc gacgaagccg accggcatgc cgacgatcgc ggcgggacgc
      721 ggcgcccccg cggcgatcgc ctcgatgaga cggaacaagg ccgtcggcgc attgccgatg
      781 gcgaccagcg atccgccgag acgcggaagc cagagatcga tcgccgccgc cgtgcgcgtc
      841 gtgccgagtt ttgcagcgag cgcggggacg gagggatcgc cgagcgtgca gatgacgtcg
      901 ttgtcccgag gcaggcgcgc ccgcgtgatc ccttcggcga ccatgcgcgc atcacagaag
      961 atgggcgcgc cgccgcgaag ggccgcgcgc gcttcctccg ccgcatgcgg cgagaacacc
     1021 atgtcgcggg ctgtctccac cataccgcag gcatggatga cgcgcaccgc gacccgctct
     1081 tcgagggcag agaagcgctg aaggtcggcc tcgccgcgga taatcgcgaa cgaccggcgg
     1141 tagatcgccg ccgggtcctt ctcgtaggcg taagctggct tcacggccgc cccctggggc
     1201 caaacataag cgtgccttcg ccagcgagga gcgcggcgac ttccgcgctc ttgaggccgc
     1261 ggcgggctgg cgggtcccct gccctgcccg cgacaatgag gtcatagccg gccgcggtcg
     1321 cggtcagggt cgcggcggtt gcggcgggcc tcgcgcatcc cttggcgcag ccgctgacgt
     1381 ggagaacgac gccctcgcca ttgggcagca acaccgccca ttgcgccgcg tcgtcgcgcg
     1441 cgggccgagc cccatgcatg caggccgggg cgccggggca ggcggcgacg cgaagccgct
     1501 ggtcgccaga gtcgcagatg aatccgagct tcgcgcacgc cttctccatt gcagcggcgg
     1561 cgcggggctc gaggccggcg atcatgaacg cccgccacgg ggtgagccgc aggccggtcg
     1621 cgccaagcgc ccgcgcgcgc tcgatcagcg ccttgaagcg gaccgcctcg aactcgccga
     1681 acgccgccgc agcgcccacg acgaccaagc cgccataggc gtgccccccg aggacgtcgc
     1741 tgagcgacgc gccccgcttc gagcgcgcgc ccggcttcgc ttcgcatgcg gcctccgcaa
     1801 acacggccgc ggctcccgtc tcttcgatga gcgcgcgcat ccgccgcggc gccgcctcgc
     1861 cggcgccggc gagcgccatg aacgagaggc ccagccgcgc cgcaatttcg cccgtctgga
     1921 cgggcgcgca ttccccgcag agcgcatctt caccgccgag aaacaccgcg aacgtcccat
     1981 cacgctgcgc ctcgaagcgg atgtcggcgt cgacgtcgtc gagcggcagg cgccccttgg
     2041 cgtcgaggac aatgccgaac tttgccggca actggcgcag gcgctcgtcc tccctgaggc
     2101 gcgtctccag cgccgaaacg ctcaccccca gatcgaactc ggcgtcggga tcgagatcgt
     2161 cgaccgggct tatgatgatg ttgcgcagcc gctcgatctc gggatcggcg tcgatgagcc
     2221 cggcgtctcc aaggcgcgca tgcagttcgg gcagagttgg ctcgctcaag ccgcgcaggt
     2281 gaagattagc gcgcgccgag aggaccatta gaccgttgcc gcaggccatc gcggcggcgg
     2341 cgagcgcgag cgcctgatcg agcgacagtc gcgcctgcgg cgcacgtacg cgcgccagca
     2401 gcccgtcgcc ggtctccatt ggcctcaatg cgccgggaca ccagcccttg cgataaggcg
     2461 gcggcgggcg gcgggcggca atcgcgctca cgccgcctcc gtgagcaccg aggaaagaat
     2521 gcgtgcgctc gaattgcggc gcgagcgcca gaagccgcgc ctcgccgctt cctcgaacac
     2581 gcccgccatg tgcgaggcgg cgcggcgatt ggcctgaagc aggaacgcgc gcacggaatc
     2641 gtcgccgagc gtggcgtcga acatgagatc gaattgcgtg ctctcgacgg cgtcggtcag
     2701 cgcggcgaag caatagagat tgtcgagcga ctgggcgatt tccgccgcgc cgcgatgccc
     2761 gtggcgcatc tggccggaaa tccagcgcgg gttggcggcg cgtccgcgaa gcgcgcgcgc
     2821 gatctcctgt ttcagcgtac ggatcttcgg cgcgtgggcg acggtcgagt cgagatggta
     2881 gagcgccgga gttgctcccg cttccgcggc ggcggcggcg aaaccgccct cgtgctcggc
     2941 gaaggcgtcg gcgtcgagcg cgtcctgtcc gggcaagtcc tgaacgtgaa cgaaggcctc
     3001 ggcgccggcg acccgttgcc gaaactccgc ggccgcttcg cgtgcttcgc cctcgccgcc
     3061 ataggcgtgg ctggttgcgg ccagatacgc ctccgcgagt tcggagcgcc ccgcccagtc
     3121 gccgcgagcg atgcgcgcgc cgagaccgac cccgtaggcg ccgggcgcgg cgccgaatat
     3181 tcgcgccggc tcgcgaagcg aggcgagcgg attgtccccg gcgctctctt cgagcgccgc
     3241 gacagcgcga acggcggccg cgaacagcgc gatctggctt gggaacacgt cgcggaaaag
     3301 gccggaaatg cgcaaggtca catcgacccg cggcctcccc agcatcgcga gcggcaagac
     3361 ctcgaagccg ctgacccggg tggacgccac gtcccaggtc ggccggcaga cgaggagcgc
     3421 gaaagcttgc gcgagatcgt cgccgcccgt gcgcatggtc gccgatcccc agaggtcgag
     3481 cacgatccgc tttggccatt cgccatggtc ctgcgcgtag cgctgaagga tctcttgggc
     3541 ggctcggcgg cc
//
LOCUS       DATGJW010000448         7820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_87669, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000448 DATGJW010000000
VERSION     DATGJW010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7820
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87669"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(65..997)
                     /locus_tag="VKU03_13475"
     CDS             complement(65..997)
                     /locus_tag="VKU03_13475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018482296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-keto-5-aminohexanoate cleavage protein"
                     /protein_id="HLJ72324.1"
                     /translation="MSATDKVIITCAITGAIHTPTMSPYLPITPDQIAAEAIAAAEAG
                     AAILHLHARDPETGKPDQTPEAFARFLPRIKQGTKAAINITTGGSPYMRVDERVRPAA
                     TFKPEVASLNMGSINFGLYHLLAKYKDFKFDWEKPHLEATRDLVFRNSFKDIEYILET
                     CYGNGTRFEFECYDIAHLYNLAHFADRKLVKPPFFVQSVFGLLGGIGPHPEDVAHMKR
                     TADRLFGGDYRWSVLGAGASQLRIAAQAAAMGSNIRVGLEDSLWAGRGKLATSNAEQV
                     ALARKIIEGLGKQVATPDDAREILALKGADQVAF"
     gene            complement(1023..1970)
                     /locus_tag="VKU03_13480"
     CDS             complement(1023..1970)
                     /locus_tag="VKU03_13480"
                     /EC_number="1.1.1.35"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010909855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase"
                     /protein_id="HLJ72325.1"
                     /translation="MARVAIIGIGFIGRAWAIAFARAGHQVALWDQDPAAPAKALDFI
                     ASVLPDLAANDLLDGAKPETLLARLGAERDLAAALSGADHVQENAPERLDVKKALFAE
                     LDRLAPKDAILASSTSALLPSRFTENLPGRARCLVIHPLNPPYLIPAAEIVPAPWTSA
                     EIVERARSFLVAAGQAPIVMKREIDGFVMNRLQGALLEEAFRLVADGYATAEDVDIGL
                     REGLALRWSFMGPFETIDLNAPGGVRDYVERYQGIYENLFPQMQRRVDWGGPCLQDIE
                     RARRAKVPTEKLVDRQKWRDRRLMALAAHKRRAARDIGV"
     gene            complement(1981..2781)
                     /locus_tag="VKU03_13485"
     CDS             complement(1981..2781)
                     /locus_tag="VKU03_13485"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329784.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72326.1"
                     /translation="MANAAADRSLDAGLSGRRVLVTAGAAGIGLAIVQRLLRHGARIF
                     VCDVVEATLEAFTKAHPQAKAVKADVSSEADVARMFSAVKAEFGGLDALVNNAGIAGP
                     TGAVEDIDPAEWRRCLDIGLTGQFLCTRLAVPMLKAAGGGSIVNISSAAGRYGYAFRT
                     PYSSAKFGVIGFTQSLAKELGPANIRVNAILPGIIEGPRMESVIRNRAQQLGLSYDAM
                     EKAYLDRISLRRMTSPHDVAATIAFLISDAGINISGQSIGVDGNVESL"
     gene            complement(2797..4818)
                     /locus_tag="VKU03_13490"
     CDS             complement(2797..4818)
                     /locus_tag="VKU03_13490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018482293.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72327.1"
                     /translation="MLLGRHRGLITAAAVFVLLFVLVNAISAGHLTYFDISFMASGGA
                     TLALAAVGETIVILSGGFDLSAGAVISLVNVVLASSMDPTAPHASVALWTFAGIGVGM
                     ATGAFNGVFIAFLRLQPIVVTLSTMFILQGVTLLVMEKPGGFVNADLGGFYLGDAVPG
                     HLPMPVLLLVCVALLWLYLKSTRFGAALYAVGSDADAARSAGVRVAITRLLAYIVAGG
                     CYGLAGVFISAQTGSGDPLVGNPLLLSIFAAVVLGGTRLGGGRGGPVGSIIGAYILMS
                     VVNVLLVLNVSAYYSTIAEGVILLLAVLAASISADSELAKQIRFAALRFSAWRAGALP
                     SQIGGGDRRLRRAASPSAAAGVQEPPFWIRNQHALRYALPSYVLFVLVVAATELWLGH
                     AAFHWGYWNSIIVLSSFLAILALGQGAVILTGGLDLSVPWTIGLSGILLAGIVQGSDQ
                     ALLYALPIVIVIALAIGFVNGVGVVALGVSPIVMTLATNGILQGAALLYSNGTPAGFS
                     SPLLRWFMTGRLLGATPVVFFIILFVVVAVTLLAQTPFGRRVYAVGNGARAARLSGVP
                     VGRTLISVYMLSSLCAGLVGILLTGFSGQASLGMGDEYLLPSIAVVVVGGAIITGGRG
                     HYLGMLGGVLLLTALQTMLAGSNLPYATRAIVYGLVVLGAVIALRERRD"
     gene            complement(4875..6404)
                     /locus_tag="VKU03_13495"
     CDS             complement(4875..6404)
                     /locus_tag="VKU03_13495"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ72328.1"
                     /translation="MPNESAVAIKGLRKAFGATLAVDDVTFAIEAGAAHALLGENGAG
                     KSTVVKLLSGLIEPDRGAISLFGEEARLRSPRAAHAYGVETAFQEMTLAPDLTVLDNM
                     LLPYAPTGWTGMIRRRAAETAVRAHFDALGFDVELWREVGDLDLAVQQKIEIARAIFR
                     KPRILLLDEPTSTLAGRDVEWLGQVIARLKKQGVTILFISHRMREVRAFCDRVTILRN
                     GRHIATAASSDLSDAEIIEMIVGRSIAQTFPPRPARPPSSEAPETLGARDLAAGAKLK
                     AASFSLRKGEILGVAGLQGMGQLDLFLACFGMTDIHRGQVLIDGRPTAIASPTDALKP
                     NVGLGFLPEDRKTEALFLELNGRHNASLPVIERFCRFGLAQGKREKNAVAEAFARVEV
                     DKRALWTPVKAFSGGNQQKIAIAKWLLAESRILLLFDPTRGIDVGTKHELYILMRAYA
                     EAGGSILFHSTEIPELVHLCDRVVVLYGGRIAAEIGAGALSENEIMRAALGGEATEKA
                     A"
     gene            complement(6650..7771)
                     /locus_tag="VKU03_13500"
     CDS             complement(6650..7771)
                     /locus_tag="VKU03_13500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329780.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ72329.1"
                     /translation="MTGGARWLLAGALALGFAAPQAADAAQKHKIFLSMSYIGNDWQA
                     EAANMVKAMASHKSLADKVSLEVQVAGPNAQRQIQQINAMVQAGAEAIVVYPISPTAL
                     NQVVKNACEKGVKIFAYDAEITEPCAYNIHIDQEEAGRKTAEWLVDKLGDKGGNIVVI
                     TGVPGTSVDTLRTKAAKEVFAKYPNIKIVAEAVGMWSQAVARTELSKILATQNWDHID
                     GLWMQVGCYTANAMQLEAGKKPNQLIPCAGEGSNGGRIQMLPIGTQVEGAASPYAPLG
                     APRISYASPPYSGALALKLAVEALEGKDVAKTTILPLPLVTNATVKLCQEGTWEEMKA
                     GCNAFKPSLVPNPGWFASIFSEQTPEVGLEAALVGQPEK"
BASE COUNT         1389 a         2502 c         2675 g         1254 t
ORIGIN      
        1 cgacgagaac ttcgattcgc atgatctcct cccgcttgac cggcgcgccg tcatcggatc
       61 tggcctaaaa cgcgacctga tcggcgccct tcagcgcgag aatctcccgc gcgtcgtccg
      121 gcgtcgccac ctgcttgccc agcccttcga tgatcttcct ggcgagggcg acctgctcgg
      181 cgttggacgt ggcgagcttg ccgcggccgg cccagaggct gtcttcgagc ccgacgcgga
      241 tgttcgagcc catcgccgcc gcctgcgccg cgatgcgcaa ctggctggcc ccggccccga
      301 gcaccgacca gcgataatcg ccgccgaaca ggcggtcggc ggtgcgcttc atatgcgcga
      361 cgtcctccgg atgcgggccg atgccgccga gcaggccgaa gaccgactgg acgaagaaag
      421 gcggcttgac cagcttgcga tcggcgaaat gggcgagatt gtagagatgg gcgatgtcgt
      481 agcattcgaa ctcgaagcgc gtgccattgc cgtagcaggt ctcgagaatg tactcgatgt
      541 ccttgaagct gttgcggaag acaaggtcgc gcgtcgcttc gagatgcggc ttctcccaat
      601 cgaatttgaa gtccttgtat ttcgcgagga gatggtaaag gccgaaattg atcgagccca
      661 tattgagcga ggcgacttcc ggcttgaacg tcgccgccgg cctgacgcgc tcgtcgacgc
      721 gcatataggg gctcccgccg gtggtgatgt tgatcgccgc tttggtcccc tgtttgatgc
      781 gcggcaggaa gcgcgcgaaa gcctccggcg tctggtcggg cttgccggtc tcggggtcgc
      841 gggcgtgcag atgcaggatc gccgcgcccg cctcggccgc ggcgatcgcc tcggcggcga
      901 tctggtccgg cgtgatcggc agatagggcg acatggtcgg cgtgtgaatg gcgccggtga
      961 tcgcgcaagt gatgatgacc ttatccgtcg cgctcatggc ctcgttcctt tctttcaatc
     1021 gctcaaacgc cgatgtctcg cgccgcgcgc cttttatgcg cggcgagcgc catcaagcgc
     1081 cgatcccgcc atttttgccg gtcgaccagc ttttccgtcg gaactttggc gcggcgcgcg
     1141 cgctcgatat cctgtaggca aggcccgccc caatcgacgc ggcgctgcat ttgcgggaac
     1201 aggttttcgt aaatgccctg atagcgctcg acatagtcgc ggacgccgcc tggcgcgttg
     1261 agatcgatcg tctcgaacgg ccccatgaag gaccagcgca gcgccaagcc ctcgcgcagg
     1321 ccgatgtcga catcctcggc cgtcgcataa ccgtcggcga cgagacggaa ggcctcttcg
     1381 agtagggcgc cttgcagccg gttcatgacg aagccgtcga tctcgcgctt catcacgatc
     1441 ggcgcctggc cggcggcgac gaggaaggag cgggcgcgct cgacaatctc cgccgaggtc
     1501 cagggcgccg ggacgatctc cgccgccgga atgagatagg gcggattgag cggatggatg
     1561 accaggcagc gcgcgcggcc gggcaggttc tcggtgaagc gcgacggcag cagcgccgaa
     1621 gtcgagctgg cgagaatggc gtccttcggc gccagccggt cgagctcggc gaacagcgcc
     1681 tttttcacat cgagccgctc cggcgcgttt tcctggacgt gatcggcgcc cgaaagcgcg
     1741 gcggcaagat cgcgctcggc gccgagccgc gccagcagcg tttccggctt agcgccgtcg
     1801 agcagatcat tggcggcgag gtccggcaaa accgacgcga tgaaatcgag cgccttggcg
     1861 ggcgccgcgg gatcctggtc ccacagcgcg acctgatggc cggcgcgggc gaaggcgatc
     1921 gcccaagccc tgccgataaa gccgatgccg ataatggcga cgcgagccat gctcccctcc
     1981 tcacaagctc tcgacattgc cgtcgacgcc gatcgactga cccgaaatat tgatcccggc
     2041 gtccgaaatc agaaacgcga tcgtcgccgc cacgtcatgc gggctggtca tgcgccggag
     2101 cgaaatccga tcgagatagg ccttctccat cgcgtcgtag gagaggccca attgctgggc
     2161 gcggttgcgg atgacgcttt ccatgcgcgg gccttcgata atgcccggca ggatcgcgtt
     2221 gacgcggata ttggccggcc ccaattcctt ggcgaggctc tgggtgaagc cgatcacgcc
     2281 gaatttggcg gacgagtacg gcgtgcgaaa ggcgtagccg tagcggcctg cggcggaaga
     2341 gatgttgacg atcgagccgc cgccggcggc tttgagcatc ggcacggcga ggcgcgtgca
     2401 aagaaactgt ccggtcaggc cgatatcgag gcagcgtcgc cattcggcgg gatcgatgtc
     2461 ctcgaccgcg ccggtcggcc cggctatgcc ggcattgttg accagcgcgt cgaggccgcc
     2521 gaattcggct ttgaccgcag agaacatccg cgcaacatcg gcctcgctcg agacgtccgc
     2581 tttcaccgct ttcgcctgcg ggtgggcttt cgtgaaggct tcgagcgtcg cctcgacgac
     2641 atcgcaaacg aaaatgcgcg cgccatggcg cagcagacgc tggacgatgg cgaggccgat
     2701 tcccgccgcg cccgccgtca ccagcacgcg ccggccggaa aggccggcgt cgaggcttct
     2761 atccgctgct gcattggcca tggcgttctc tcatcttcaa tcgcggcgct cgcgcagcgc
     2821 gatcaccgct ccgagcacga cgaggccata gacgatggcg cgcgtcgcgt aggggagatt
     2881 gctgccggcg agcatggttt gcaaagcggt caaaagcagc acgccgccga gcatgccgag
     2941 atagtggccg cgcccgccgg tgatgatcgc gccgccgacc acgacgacgg cgatcgaggg
     3001 caggagatat tcgtcgccca tgccgaggct cgcctgtccg gaaaagccgg tcagcaggat
     3061 gccgacgagg cctgcgcaaa gcgaggacag catgtagacg ctgatcagcg tgcggccgac
     3121 cggcacgccg gagagccgcg cggctcgcgc gccgttgccc accgcgtaaa cgcggcggcc
     3181 gaatggcgtc tgcgcgagca acgtgaccgc gacgacgacg aaaaggatga tgaagaaaac
     3241 gaccggcgtc gcgccgagga gcctgccggt catgaaccat cggagcaagg gcgaggaaaa
     3301 gccggccggc gtaccgttcg aatagagcag ggccgcgcct tgcaggatgc cgttcgtcgc
     3361 gagcgtcatg acgatcggcg aaacgccgag cgcgaccacg ccgacgccgt tgacgaagcc
     3421 gatcgcgagg gcgatcacaa tcacgatcgg caaggcgtag agcaacgctt gatccgagcc
     3481 ttgcacgatt cccgcgagca gaatgccgga aaggccgatc gtccagggca cggaaagatc
     3541 gaggccgccg gtgagaatga ccgcgccctg gccgagcgcg aggatcgcca gaaagctcga
     3601 caggacgatg atcgaattcc aatagcccca atggaaggcg gcatggccga gccagagctc
     3661 ggtcgcggcg acgacaagca cgaaaagaac gtaggagggc agggcgtagc gcagagcgtg
     3721 ctggttgcgg atccagaagg gcggctcctg gacgccggcc gccgcgctcg gcgaggccgc
     3781 gcgccgcaag cgccgatcgc cgccgccgat ctggcttggc agagcgccgg cgcgccaggc
     3841 ggagaagcgc aaagcggcga agcgaatctg cttggcgagc tcggaatcgg cgctgatcga
     3901 ggctgcgagc accgccagaa gcaggatcac gccttccgcg atggtcgaat aataggccga
     3961 gacattgagt acgagcagaa cgttgacgac gctcatcagg atataggcgc cgatgatcga
     4021 gccgaccggg ccgccgcggc cgccgccgag ccgcgtgccg ccaaggacca cggcggcgaa
     4081 gatcgacagg agcagcggat tgccgaccag cggatcgccg gagccggtct gcgccgagat
     4141 gaagacgccg gcaaggccat agcagccgcc ggcgacgatg taggccaaga ggcgcgtgat
     4201 cgcgacgcgc acgccggccg agcgggcggc gtcggcgtcg ctgccgaccg catagagcgc
     4261 cgcgccgaag cgggtcgatt tcagatagag ccaaagcagc gcgacgcaga cgagcagcag
     4321 gaccggcatg ggcaaatggc cgggcaccgc gtcgccgaga tagaagccgc cgagatcggc
     4381 gttgacgaag ccgccgggct tctccatcac cagcaaggtc acgccttgca ggatgaacat
     4441 cgtcgagagc gtgacgacga tcggctgcag gcgcaggaag gcgatgaaca cgccgttgaa
     4501 agcgccggtc gccatgccga cgccgattcc ggcgaaggtc cagagcgcga cggacgcatg
     4561 cggcgccgtc gggtccatgc tcgaggcgag cacgacattg accagcgaga tcacggcgcc
     4621 ggctgagagg tcgaagccgc cgcttaggat gacgatcgtc tcgccgacgg ccgccagcgc
     4681 cagcgtcgcg ccgccgctcg ccatgaagct gatgtcgaaa taggtgaggt ggccggcgct
     4741 gatcgcgttg accaggacga acaacagcac gaagaccgcc gccgccgtaa tcaagccgcg
     4801 atggcggccg agcagcacag cgaaacctgg ccggcggcgg ccgccagaat cggagcggaa
     4861 cgcggccgag acgctcacgc ggccttctcc gtcgcttcgc cgcccagggc ggcgcgcatg
     4921 atctcgttct cgctcaaggc gccggcgccg atctcggcgg cgatgcggcc gccgtaaagc
     4981 accacgaccc ggtcgcagag atggacgagc tcgggaattt ccgtcgaatg gaacaggatc
     5041 gagccgccgg cctccgcata ggcgcgcatc aggatgtaaa gctcgtgctt ggtgccgaca
     5101 tcgatgccgc gggtcgggtc gaacagaaga aggatgcggc tctcagcgag cagccatttg
     5161 gcgatggcga tcttctgctg attgccgccg gagaaggcct tgaccggcgt ccacagcgcg
     5221 cgcttgtcga cctcgacgcg cgcgaaggcc tcggcgaccg cgtttttctc gcgcttgcct
     5281 tgcgcgaggc caaagcggca aaagcgctcg atcaccggca gcgaggcgtt gtgacggccg
     5341 ttcaattcga gaaacaacgc ctccgtcttg cggtcctcgg gcaaaaatcc gagcccgaca
     5401 ttcggcttca aggcgtcggt cggcgaggcg atggcggttg ggcggccgtc gatgaggacc
     5461 tgcccgcgat gaatgtcggt catgccgaaa caggcaagga acagatcgag ctggcccatg
     5521 ccctgcagcc cggcgacgcc gagtatctcg cccttgcgca aggaaaagct cgccgccttg
     5581 agtttggcgc cggcggcgag atcgcgcgcg ccgagcgttt ccggcgcttc gctcgaaggc
     5641 ggccgcgccg ggcgcggcgg aaacgtttgc gcgatcgagc ggccgacgat catctcgatg
     5701 atctcggcgt cggagagatc gctcgaagct gcggttgcga tatggcgccc gttgcgcagg
     5761 atcgtcaccc ggtcgcagaa agcgcggact tcgcgcatgc ggtgcgaaat gaacaggatt
     5821 gtgacgccct gctttttcaa gcgggcgatc acctggccga gccattcgac gtcgcggccg
     5881 gccaaggtgg aggtcggctc gtcgagcagg agaatgcgcg gcttgcggaa gatcgcccgg
     5941 gcgatctcga tcttctgctg aacggcgaga tcgagatcgc cgacctcgcg ccacaattcg
     6001 acgtcgaagc cgagcgcgtc gaaatgggcg cggaccgccg tctcggcggc gcggcggcgg
     6061 atcatgccgg tccagccggt cggcgcgtag ggcaggagca tattgtcgag cacggtgagg
     6121 tccggcgcga gcgtcatctc ctggaacgcg gtctcgacgc cataggcgtg cgcggcgcgc
     6181 ggcgatcgca gccgcgcctc ctcgccgaac aggctgatcg cgccccggtc gggctcgatc
     6241 aagccggaca gaagcttgac gacggtggat ttgccggcgc cgttctcgcc gagcaaggcg
     6301 tgcgcggcgc cggcctcgat ggcgaaggtg acatcgtcga cggccagcgt cgccccgaaa
     6361 gccttgcgca ggcccttgat cgcgacggcg ctttcgttcg gcatgattcg gcgctcattg
     6421 cccaatgcgg acgtacctat cgcgaggagg ataccaactc cgccggcgtg gcttaccctc
     6481 atccggccct tcgggtcccc ttctcccgcg agcgggagaa ggggctgtag ccgcggtcgg
     6541 cggccgcggc tacaaacgac cgctggataa agcccgctac gcgtcccctc tcccgcttgc
     6601 gggagagggt tggcgagggc gagccacggc cttgcagtac aacagaagcc tacttctccg
     6661 gttggccgac caaagccgct tcgaggccga cttccggggt ctgctccgag aagatcgagg
     6721 cgaaccagcc gggattgggc acgagcgagg gcttgaacgc gttgcagccc gccttcatct
     6781 cctcccaggt gccttcctgg cagagcttga ccgtcgcgtt ggtgacgagc ggcagcggca
     6841 gaatcgtggt cttggcgacg tccttgcctt ccagcgcttc gaccgcgagc ttcagcgcca
     6901 gggcgccgga atagggcggc gaggcgtagg agatgcgcgg cgcgccgagc ggcgcataag
     6961 gcgaggcggc gccttcgacc tgcgttccga tcggcagcat ttggatgcgg ccgccattcg
     7021 agccttcgcc ggcgcagggg atgagttggt tcggcttctt gccggcttcg agctgcatgg
     7081 cgttggcggt gtagcagccg acctgcatcc agagcccgtc gatatggtcc cagttctgcg
     7141 tggcgaggat cttcgacagt tcggtgcgcg ccaccgcctg gctccacatg ccgaccgcct
     7201 cggcgacgat cttgatgttc ggatacttgg cgaagacctc cttcgccgct ttggtgcgca
     7261 atgtgtcgac cgaggtgccc ggcacgccgg tgatgacgac gatgttgccg cccttgtcgc
     7321 cgagcttatc gaccagccat tccgccgtct tccgtccggc ctcttcctgg tcgatatgga
     7381 tgttgtaggc gcagggctcg gtgatctcgg cgtcgtaggc gaaaatcttg acgcccttct
     7441 cgcaggcgtt cttgaccacc tggttgagcg cggtcggcga gatcggatag acgacgatcg
     7501 cctcggcgcc cgcctggacc atggcgttga tctgctgaat ttgccgctga gcgttcggcc
     7561 cggccacctg cacttcgagg ctgaccttgt cggcgagact cttatggctg gccatggcct
     7621 tgaccatatt ggcggcctcg gcctgccaat cgttgccgat atagctcatg ctgagaaaaa
     7681 tcttatgctt ttgggcagcg tcagccgcct gcggcgcggc gaaaccgagc gccagcgcgc
     7741 ccgccaaaag ccaccgagct cctcccgtca tgatttctct cccgcgtttt tgcgctgccg
     7801 gttcttgccg gcagatttgt
//
LOCUS       DATGJW010000449         3082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_815686, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000449 DATGJW010000000
VERSION     DATGJW010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3082
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_815686"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..494)
                     /gene="coaE"
                     /locus_tag="VKU03_13505"
     CDS             complement(<1..494)
                     /gene="coaE"
                     /locus_tag="VKU03_13505"
                     /EC_number="2.7.1.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008873253.1"
                     /note="Dephospho-CoA kinase (CoaE) performs the final step
                     in coenzyme A biosynthesis; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004140 - dephospho-CoA kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015937 - coenzyme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dephospho-CoA kinase"
                     /protein_id="HLJ72330.1"
                     /translation="MIVIGLTGSLAMGKSTVAAMLREAGAPVFDSDEAVHLLYRGADA
                     AEIEQAFPGVTIDGVVDRGRLAEKVLNDPAAFARLEAIVHPKVAAARDQFLQRAAQAG
                     RRVAVADIPLLFESSAEKSVDLVVVVSAPESVQKARALSREGMTEKRFAAIVAKQMPD
                     SEKRR"
     gene            complement(491..1354)
                     /locus_tag="VKU03_13510"
     CDS             complement(491..1354)
                     /locus_tag="VKU03_13510"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173974.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0019632 - shikimate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HLJ72331.1"
                     /translation="MTERPALLRACIVGWPVAHSRSPLIHGFWLHSLGLAGAYDREAV
                     PPGAFASFAASIGKRGLVGANVTIPHKEAAYAACDKLTATAAELGAVNTLWREDGALW
                     GDNTDVAGFLANLDEEAPGWRGGAETVVLIGAGGAARAVISGLLSRAVKRIAIVNRTA
                     ERAQRLAAHFGAGISVHAPADLPRLLERAGLLVNTSSLGMAGQPALDIDLSPLAASAI
                     VADLVYVPLQTPLLAAARRRGLRVVGGLGMLLHQAAPGFERWFGVKPVVSPELRALIE
                     ADLVKTSERRP"
     gene            complement(1351..2010)
                     /locus_tag="VKU03_13515"
     CDS             complement(1351..2010)
                     /locus_tag="VKU03_13515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013168584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HLJ72332.1"
                     /translation="MPIDEAARESAAQPSPPPLWRGPRLVLASSSAGRAALLRSAGLD
                     FAVDPARLDERAVEEEFRRAGGAASGLAARLAEAKAIEVSARHPGALCLGADQTLTLG
                     ERVLHKPKDMAAARDHLQLLAGRVHRLISAFCFARNGRALFQAADEARMAMRPLDPGA
                     LSLYLSLAGEAALASVGAYQAEGLGVHLFERIEGDHATIIGLPLLPVLAWLRRQGYLA
                     L"
     gene            complement(2019..2852)
                     /locus_tag="VKU03_13520"
     CDS             complement(2019..2852)
                     /locus_tag="VKU03_13520"
                     /EC_number="2.7.11.33"
                     /EC_number="2.7.4.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173976.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004674 - protein serine/threonine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA];
                     GO_process: GO:0006470 - protein dephosphorylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, water dikinase regulatory protein"
                     /protein_id="HLJ72333.1"
                     /translation="MGRSYFHLHLVSDATGETLIAASRAAAAQYQGVASIEHVYPLVR
                     SHAQLDRVIAEIDNAPGIVLFTLVDPELSRRLEASCEASGSPCLSVLSPILNLFQSYL
                     GAVSTPRAGAQHMLNAEYFRRIDALNFTMMHDDGQLTENLEQADIVLLGVSRTSKTPT
                     SIYLANRGFKTANVPLVPNVPLPPAIENLRNPLPVGLIASPERIVQIRQHRLLALNAD
                     QQTPYIDRMAVAEEIAHSRRLFADRGWSVIDVTRRSIEETAAAIIDLYREHNLRFIAQ
                     A"
BASE COUNT          531 a         1008 c         1085 g          458 t
ORIGIN      
        1 cggcgcttct cgctatccgg catttgcttg gcgacgatgg cggcgaatcg cttctcggtc
       61 atcccctccc ttgagagagc gcgcgccttt tgcacagatt ccggcgcact gacgacgacg
      121 acgagatcga ccgacttttc cgccgacgat tcgaagagaa gcggaatgtc ggcgaccgcg
      181 acgcgccgcc ccgcttgcgc agcccgttgc aggaattggt cacgcgcggc ggcgactttc
      241 gggtgcacga tcgcctcgag ccgggcgaag gcggccgggt cgttcagcac tttctcggcg
      301 agacggccgc gatcgacgac gccgtcgatc gtcacgccgg gaaaggcctg ctcgatctcg
      361 gccgcgtcgg cgccgcgata gagcaggtgg accgcttcat ccgaatcgaa aaccggcgcg
      421 ccggcctcgc ggagcatcgc cgcgaccgtc gatttgccca tggcgagcga gccggtcagt
      481 ccgatgacga tcaaggccgg cgctcgctgg ttttgacgag atcggcttcg atcaacgccc
      541 tcagctccgg cgaaacgacc ggcttgacgc cgaaccatcg ctcgaagccg ggcgcggcct
      601 gatgcagcag catgcccaag cctccaacga cgcgcagccc gcgccggcgc gccgcggcga
      661 ggagcggggt ttgcagagga acatagacga gatcggcgac gatcgccgac gcggcgagcg
      721 gcgaaagatc gatgtcgagc gccggctggc cggccatgcc gagcgaggac gtgttcacga
      781 gaaggccggc gcgttcgagc aggcgcggca gatcggcagg cgcgtggacc gaaatcccgg
      841 cgccgaaatg ggcggcgagg cgctgcgcgc gctccgccgt ccgattgacg atggcgattc
      901 gcttgacggc gcgcgacagc aacccgctga tcaccgcccg cgccgcgccg cccgcgccga
      961 tcagcacgac tgtctcggcg ccgccgcgcc agcccggcgc ttcctcgtcg agattggcga
     1021 gaaacccggc gacatcggtg ttgtcgcccc agagcgcgcc gtcttcgcgc cacagcgtgt
     1081 tgacggcgcc gagctcggcg gcggtcgccg tcagcttgtc gcaagccgcg taggcggctt
     1141 ccttatgcgg aatggtgaca ttggcgccga ccaatccgcg cttgccgatc gaggcggcga
     1201 aagacgcaaa agcgcccggc ggcacggctt ctctgtcgta agcgccggca agcccgaggc
     1261 tatgcagcca gaagccgtga ataagcggcg agcgggaatg cgcgacaggc cagccgacaa
     1321 tgcaagcccg cagcagcgcg ggacgctccg tcatagcgcc aaatatcctt gccggcgcag
     1381 ccaggcgaga accggcagca ggggcaagcc gataatcgtc gcgtggtcgc cttcgatgcg
     1441 ctcgaacaag tggacgccga gcccttccgc ctggtacgcg ccgacgctgg cgagcgccgc
     1501 ctcgccggcg agcgagagat aaaggctgag cgcgcctggg tcgagcggcc gcatcgccat
     1561 ccgcgcttca tccgccgcct ggaacagggc tcggccgttg cgggcgaagc agaaggcgga
     1621 gatcaggcgg tggacgcggc cggccagcaa ttgcagatga tccctggcgg cagccatatc
     1681 cttcggctta tgcaagacgc gctcgccgag cgtcagcgtc tggtcggcgc cgaggcacag
     1741 cgcgcctggg tggcgggcgc tgacctcgat cgccttggct tcggccaggc gagcggcgag
     1801 gccggacgcc gccccgccgg cccgccggaa ttcctcctcg acggcgcgct cgtcgagccg
     1861 cgccgggtcg accgcaaaat caagcccggc gcttcgcagc agcgcggccc ggccggcgct
     1921 ggacgaggcc aggacgaggc gaggtccgcg ccagagaggc ggcggcgaag gctgcgcggc
     1981 gctctcgcgc gccgcttcgt cgatcggcat ggcggcgttc acgcttgagc gatgaagcga
     2041 agattgtgtt cgcggtagag atcgatgatc gccgccgccg tctcctcgat cgagcggcgc
     2101 gtcacgtcga tcaccgacca gccgcgatcg gcgaacaggc ggcgggaatg ggcgatctcc
     2161 tcggccaccg ccatccggtc gatatagggc gtctgctggt cggcgttgag cgccagcagg
     2221 cgatgctggc ggatttgcac gatccgctcc ggcgaagcga tcagcccgac cggcagggga
     2281 ttgcgcaagt tttcgatcgc cggcggcagg gggacattcg gcaccagcgg aacgttggcg
     2341 gtcttgaagc cgcgattggc gagatagatg ctggtcggcg tcttcgacgt gcggctgacg
     2401 ccgagcagca cgatgtcggc ctgctcgagg ttttcggtga gctggccatc gtcatgcatc
     2461 atggtgaagt tcagcgcgtc gatgcgccgg aaatattcgg cgttgagcat gtgctgcgcg
     2521 ccggcgcgcg gcgtcgagac ggcgccgaga taggattgaa acaggttcag gatcggactc
     2581 aagaccgaga gacagggcga accggacgct tcgcaactcg cttcgagccg gcgcgacaat
     2641 tccggatcga ccaacgtgaa cagcacgatg ccgggcgcat tgtcgatttc cgcgatcacc
     2701 cggtcgagtt gcgcgtggct gcgcaccagc ggatagacat gctcgatcga cgcgacgccc
     2761 tgatattgcg ccgccgcggc gcgcgaagcc gcgatcagcg tctcgccggt cgcgtcggag
     2821 acgagatgaa gatggaaata gctgcgtccc aatcctgtgg cgcctttcgc gtttagatgg
     2881 cttatagccg gggggcgagc ctgtggacaa atggggaccg cagggtccgc gctcggcctg
     2941 gccggcgccg gcccgggaaa ggccttgaag aagcccacag gcctgcggcg cgggaaaatt
     3001 cggcccacgt cgcgcgggaa aaatctcgcg cgtcgttttc gcccgccaat aaacatgacg
     3061 attcggtttt tgcggcctga aa
//
LOCUS       DATGJW010000450         3291 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1333543, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000450 DATGJW010000000
VERSION     DATGJW010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3291)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3291)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3291
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1333543"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..723)
                     /locus_tag="VKU03_13525"
     CDS             complement(<1..723)
                     /locus_tag="VKU03_13525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012252692.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HLJ72334.1"
                     /translation="MADYYPLLAKALAGLPSTTTPDARRAIYDRARKALIGQLRSLKP
                     PLSEDVIAREEAALDAAVARLEGERGAPPPAAAPEPAAAPNPPAAAPPPPAATPAASR
                     PTAQTPRPMTAPANPPPRPQTPPPATARPTPPAAGASARPSGSPAAPPPRPAASGATL
                     RYAPPPRPVAPPPIPGPAQVPLRPPGAGAPPPAAAPAGPPTPPPLDSPAAWTPGAPPP
                     VETPDAGFVEQNYAPPIAAAYPE"
     gene            complement(764..2059)
                     /locus_tag="VKU03_13530"
     CDS             complement(764..2059)
                     /locus_tag="VKU03_13530"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthase"
                     /protein_id="HLJ72335.1"
                     /translation="MANVVVVGAQWGDEGKGKIVDWLSLSADVVVRFQGGHNAGHTLV
                     IDGKTFKLALLPSGIVRPGKLSVIGNGVVLDPHYLADEIEKIRGQGVAVTPDNLKIAE
                     NIPLILPLHRELDAHRESSNVAGVKIGTTKRGIGPAYEDKVGRRAIRLMDLAEPDALD
                     AKIDRLLAHHDPLRRGLGLEPADRAAIRKDLMAVAPKILPFMAATYDLLDRERRAGKR
                     ILFEGAQGALLDVDHGTYPYVTSSNTVAGSAAAGSGLGPGAIGYVLGIAKAYTTRVGE
                     GPFPTELDNETGRLIGKRGNEFGTNTGRPRRCGWFDAVLVRQCVKTSGIDGLALTKLD
                     ILDGFDEIKVATHYRLDGETVDYLPAAQGAQARVEPVYETIEGWKGATGGARSWADLP
                     AQAIKYVRRIEELVGAPVALLSTSPEREDTILVHDPFRD"
     gene            complement(2215..2352)
                     /locus_tag="VKU03_13535"
     CDS             complement(2215..2352)
                     /locus_tag="VKU03_13535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72336.1"
                     /translation="MAMSADGASGDSGNAVKLVLAWAFVGVPLLWGVWNTLMAALQLF
                     K"
     gene            complement(2371..>3291)
                     /locus_tag="VKU03_13540"
     CDS             complement(2371..>3291)
                     /locus_tag="VKU03_13540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="OFA family MFS transporter"
                     /protein_id="HLJ72337.1"
                     /translation="PDRPGMATGMAIMGFGGGAFIASPLSVFLMRAFTTPTHIGVAET
                     FVVLGAIYFCFMMVGAVIVRIPAPGWKPEGYVAPAQPKKLITTKNVFVYQALKTPQFW
                     LIWWVLCLNVTAGIGVLGQASAMSQEMFPGRITPVAAAGFVGLMSLFNMGGRFAWASI
                     SDYIGRKNTYFIFFVLGFVLYAIVPYAGGTGNVALFVACFLIIISMYGGGFSTVPAYL
                     RDMFGTQYVGAIHGLLLTAWSMAGIFGPVLVNYIRQYNVTHGVARAQAYNVTMYVMDA
                     LLVIGFICNFLVREVDAKHHLAMREADATA"
BASE COUNT          561 a         1071 c         1149 g          510 t
ORIGIN      
        1 ttcgggatag gccgccgcga tcggcggcgc gtaattctgt tcgacgaatc cggcgtcggg
       61 cgtctcgacc ggcggcggcg ctccgggcgt ccatgcagcg ggcgaatcga gcggcggcgg
      121 cgttggcggg ccggccgggg cggcggccgg cggcggcgcc ccggcgcctg gaggccgaag
      181 cggaacttgc gccggccccg gaatcggcgg cggcgcgacc ggccggggtg gcggagcgta
      241 acgcagcgta gcgcctgaag cggccggccg cggcggcggc gcagcgggag agccgctcgg
      301 ccgggcgctc gcgccagcgg ccggcggcgt gggccgcgcg gtcgcaggcg gcggcgtttg
      361 cggccgcggc ggcggattcg ccggggcggt catcggccgc ggcgtctggg ccgtcgggcg
      421 cgaggcggcc ggcgtcgccg ccggaggcgg cggggcggcg gccggcgggt tcggggcggc
      481 ggccggttcg ggcgcggcag ctggaggcgg cgcgccgcgc tcgccttcga ggcgggcgac
      541 ggcggcgtcc aaggccgcct cttcgcgcgc gatcacgtct tccgatagcg gcggcttcag
      601 cgagcgcaat tggccgatca gcgccttgcg ggcgcggtca taaatcgcgc gccgcgcgtc
      661 aggcgtcgtc gtgctcggca aaccggcgag cgctttggcc aaaagcggat aataatcagc
      721 catgttcggg ttcggtcatc ctgtctcgcc cccgcgagag gccttagtcc cgaaacggat
      781 catgcacaag aattgtgtcc tcacgttccg ggctggtgga caataaggcc accggcgcgc
      841 cgaccagttc ttcgatccgg cggacatatt tgatcgcctg ggcgggcaga tcggcccagg
      901 agcgggcgcc gccggtcgcc cccttccagc cctcgatcgt ttcatagacc ggctcgactc
      961 gcgcctgcgc gccttgcgcg gccggcaggt aatcgacggt ttcgccgtcg agccgatagt
     1021 gggtcgcgac cttgatctcg tcgaaaccgt cgaggatgtc gagcttggtc agggcgaggc
     1081 cgtcaatgcc ggaggttttc acgcattgac gcaccagaac cgcgtcgaac cagccgcagc
     1141 ggcgcggccg cccggtattg gtgccgaatt cattgccgcg cttgccgatc agccggccgg
     1201 tctcgttgtc gagctcggtc gggaaagggc cttcgccgac cctggtcgta tacgccttgg
     1261 cgatgccgag cacatagccg atcgcgccgg ggccgagacc ggaccctgcc gccgcgctgc
     1321 cggccaccgt gttcgacgat gtgacataag ggtacgtccc atgatcgacg tcgagcagcg
     1381 cgccttgcgc gccttcgaac aggatacgct ttccggcgcg gcgctcgcgg tcgagcaggt
     1441 cataggtcgc cgccatgaaa ggcaggattt tcggcgcgac cgccatcaag tccttgcgga
     1501 tcgcggcgcg gtcggctggc tccaggccga gaccgcggcg caacggatcg tgatgagcga
     1561 gaaggcggtc gatcttggcg tcgagcgcgt ccggctcggc aaggtccatc aggcggatcg
     1621 cccgccttcc gaccttgtcc tcataggccg ggccgatgcc gcgcttcgtc gtgccgatct
     1681 tcacgccggc gacattggag gattcgcgat gggcgtcgag ctcgcgatgc agcggcagga
     1741 tcagcgggat gttctcggca attttgagat tgtccggcgt caccgcgacg ccttggccgc
     1801 ggattttctc gatctcgtcg gcgaggtaat gcggatcgag cacgacgccg ttgccgatca
     1861 ccgacaattt gccgggacgg acgatgccgg acggcagcag cgccagcttg aatgtcttgc
     1921 cgtcgatgac cagcgtgtgg ccggcattgt ggccgccttg gaaacgcacg accacgtcgg
     1981 cggaaaggga cagccagtcg acgatcttgc ccttgccttc gtcgccccac tgagcgccga
     2041 ccaccacgac attggccatt caagaacgcc tcaaaaggaa gccccggcgc gggcaacaag
     2101 cggcggcgcc ggggcttatt gcggcaaggt cctacgcgat ctaggcgcgg gagtaaaggc
     2161 gggcggcgcg atcggcgccg ccctgcgaca tcgccgcccg taatggcggc cgctttattt
     2221 gaacaattgc agcgcggcca tcagcgtgtt ccagacgccc cagagaagcg ggacgccgac
     2281 gaaagcccag gccaggacaa gctttacggc gttgcccgaa tcgcccgacg cgccgtccgc
     2341 actcatcgcc atgatcctcc cttaagcgct ttaagccgtc gcgtcggctt cgcgcatcgc
     2401 cagatggtgc ttggcgtcga cttcccgcac caggaaattg cagatgaagc cgatcaccag
     2461 aagcgcgtcc atcacataca tcgtcacgtt ataggcctgc gcccgcgcga cgccgtgggt
     2521 gacgttgtac tggcggatgt agttgaccaa aaccgggccg aaaatgccgg ccatcgacca
     2581 cgcggtcaga agaaggccgt ggatggcgcc gacatattgc gtgccgaaca tgtcgcgaag
     2641 ataggccggc accgtcgaga agccgccgcc atacatgctg atgatgatca ggaagcaggc
     2701 gacgaagagc gcgacattgc cagttccgcc ggcgtaaggc acgatcgcat aaaggacgaa
     2761 gcccagaacg aagaagatga aataggtatt cttgcgcccg atgtaatccg agatcgaagc
     2821 ccaggcgaac cgtccgccca tattgaacag gctcatcagg ccgacaaagc cggccgccgc
     2881 gaccggcgtg atccgtcccg gaaacatttc ctggctcatc gcggaggcct ggccgagaac
     2941 gccgatgccg gccgtcacgt tgaggcagag cacccaccag atcagccaga attgcggcgt
     3001 cttcagcgcc tgatagacga acacgttctt tgtggtgatc agcttcttcg gctgcgccgg
     3061 cgcgacatag ccctcgggct tccagcccgg cgccggaatc cgcacgatca ccgcgccgac
     3121 catcatgaag cagaaataga tcgcgccgag gacgacgaac gtctcggcga cgccgatgtg
     3181 agtgggggtg gtgaacgcgc gcatcaggaa caccgacaaa ggcgaggcga tgaaggcgcc
     3241 gccgccgaag cccatgatcg ccatgccggt cgccatgccc ggccggtcgg g
//
LOCUS       DATGJW010000451         8974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_605601, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000451 DATGJW010000000
VERSION     DATGJW010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8974
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_605601"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(383..2035)
                     /locus_tag="VKU03_13545"
     CDS             complement(383..2035)
                     /locus_tag="VKU03_13545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HLJ72338.1"
                     /translation="MPVSDDPQSQPRAIGGRAPDVFRPGGWVPMRCYSEDEDVDFCIV
                     GTGAGGGTLACKLAEYGFSVVALDAGPYWRPLEDFASDETHQAKLYWTDERIVDGENP
                     LQLGANNSGKSVGGSTVHFAMVSLRFRPEWFKARSKLGYGADWPLDWREMWSYYREVE
                     QTLKISGPVTYPWGPRRPRYPYRPHELNAAALVLAEGAEAMGIAWSPTPLATISAPRG
                     LAHPCVYRGMCVIGCSTNAKQSVLLTWLPRALAAGAEIRDLSMAGWIEHDEAGRATGV
                     HYHRQDGWRFQRARNVVVACYSIETPRLLLNSASELFPDGLANSSGLVGKNLMVQANQ
                     AVWGLMEREIRSYKGPPSLALTEHWNYQDEGKDFFGGYAYMSQGPLPLVWSGVESSKR
                     GLWGQRLIDEMEKYNHQVGLKMVGEFMPQERNRVTLADEKDQYGLPIPRVTYSYCDND
                     KQMIKHALGFMRQALQAAGATDIWDETDDTCHLNGTARMGDDPATSVVNADCRSWDIP
                     NLWICDGSVFPTVGGVNPSLTIQAIACRTADRIKALARRGDL"
     gene            complement(2019..2663)
                     /locus_tag="VKU03_13550"
     CDS             complement(2019..2663)
                     /locus_tag="VKU03_13550"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010683335.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconate 2-dehydrogenase subunit 3 family
                     protein"
                     /protein_id="HLJ72339.1"
                     /translation="MPDRYPGYDVLNKRNSPSWNDKTRAVIDKRLALAADEHRFFSDA
                     EWPTMHALCERIVPQPPERKNRAPIAAMVDEKLFENRTDGYRDSRLPSMREAWRRGLA
                     ALDAEAVRDGRRFHELTIKEQEAILAKVQKGEAHDEAWGDMPPALFFEKRLLHDIVSA
                     YYGHPSSWSEIGFGGPASPRGYVRMNFDRRDPWEASEARPGREEKARRENARVG"
     gene            complement(2656..3588)
                     /locus_tag="VKU03_13555"
     CDS             complement(2656..3588)
                     /locus_tag="VKU03_13555"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72340.1"
                     /translation="MIRRPTDILEALTPTSGKALRALPALPQAARLPAIFRRRGSDAA
                     AARQLNQGAAILALSVLADSAIEHYRGSFENRAMYAPLVASALALAASLAASTEERPQ
                     PRLWRDAIYGAATAVGVAGLGFHIYNIAKRPGGFSWLNFFYAAPIGAPGALVMAGLLG
                     RGAERVRKRSRRPMKRLGLPLGRLLALISATGIAGAVGEAGLLHFRGAFQNPAMILPL
                     SAPPVASALLGASALQRQGPRRLARWWLALTALLGFIGSGFHVYGVSRNMGGWRNWSQ
                     NVLNGPPLPAPPSFAGLALAGLAALLLIENEADA"
     gene            complement(3585..4703)
                     /locus_tag="VKU03_13560"
     CDS             complement(3585..4703)
                     /locus_tag="VKU03_13560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008965442.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enolase C-terminal domain-like protein"
                     /protein_id="HLJ72341.1"
                     /translation="MTAARPDAPIAGLSAAAYRIPTDAPEADGTFEWSATTLVVARIE
                     AAGQSGLGYTYAGASAAVLAREQLAEVLQGRECFDIPGALAAMRRKVRNLGRSGVAAC
                     AISAVDAALWDLKARLLDLPLARLLGRCRDHVPIYGSGGFTSYSDERLKEQLAGWAER
                     DGCRFVKMKIGSEPERDPRRARAAKSAIGDTQLFVDANGAFSVKQALRFIEACNDLDI
                     RWFEEPVTSDDPAGLRLVRDRTPAQTEVAAGEYIFTLDDARHLLEAGAVDVLQADATR
                     CGGVSGFLEVAALCQAHHIDLSGHCAPALHRHAACAAPRLRHLEWFHDHVRIEQMLFD
                     GAPVAKDGAIRPDLSRPGLGLDFKAQDAEQFRIDGAIR"
     gene            complement(4700..6493)
                     /locus_tag="VKU03_13565"
     CDS             complement(4700..6493)
                     /locus_tag="VKU03_13565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007535720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HLJ72342.1"
                     /translation="MAQTVGDFIVERLHAWGVRKMFGYPGDGINGVFGALNRAKGKIE
                     FIQARHEEMAAFMASAYAKFTGELGVCIATSGPGASHLITGLYDARLDHMPVLAIVGQ
                     QARNAMGGHYQQEVDLASLFKDVAGAFVQQASSPPQVRHLVDRAIRIAIAERRVTAII
                     LPNDLQEETYREPPRKHGSVHSGVGFARPKIEPYETDLRRAAAVLNAGSKVAILVGAG
                     ALRATDEVIAIADKLGAGVAKALLGKAALPDDLPFVTGSIGLLGTKPSYDMMMGCDTL
                     LMIGSGFPYSEFLPEEGQARGVQIDIAPDMLSLRYPMEVNLVGDAAETLRLLLPMVVH
                     KQDRGWRDEIEKNVKEWWKTLEDRAMQPANPVNPQRVTWELSPRLPDGVILTSDSGSC
                     ANWWARDLKIKRGMMASLSGGLASMGAAVPYAIAAKFAHPDRPVVALVGDGAMQMNNM
                     AELITVAKYWRKWSNRQWIVCVFNNEDLNQVTWEQRVMNGDPKFNASQEIPNVPYHRF
                     AELIGLKGIYVDDPEKLGAAWDEALASRRPVLLEVKTDPEVPPLSPHITLEQAKKFTE
                     TLIKGDPREGSVIRETARQVLSAVLPGGDKK"
     gene            complement(6665..7660)
                     /locus_tag="VKU03_13570"
     CDS             complement(6665..7660)
                     /locus_tag="VKU03_13570"
                     /inference="COORDINATES: protein motif:HMM:NF013215.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA topoisomerase IB"
                     /protein_id="HLJ72343.1"
                     /translation="MSARLRKIAVSELDIERRRVGRGFAYFDSAGRLIKDPATLARIR
                     KMAIPPAYSDVRIAFDERAHLQAIGRDAAGRWQYRYHPDWTDVREKRKVGRMAELVAA
                     LPKIRAAVKRDVAAKELSCEKAAACAVAMIDESCIRVGCEDYARADGGRGAATLLKQH
                     AAIGRDQIKLQFRGKGGRTIEREIADPALARALRRLAAIPGRRLLQFLDKEGRPRALT
                     AADVNAYLQRVSGAAVSAKDFRMTNASALAAEELAAVAPAASETGQRRQLSSVMRLVA
                     EQLANTPAIARKSYVHAIVEKSFRRGALAKTMKRAKPAAFRTRAESLLGELARAE"
     gene            8102..8494
                     /locus_tag="VKU03_13575"
     CDS             8102..8494
                     /locus_tag="VKU03_13575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091631.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042314 - bacteriochlorophyll binding
                     [Evidence IEA];
                     GO_process: GO:0019684 - photosynthesis, light reaction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HLJ72344.1"
                     /translation="MRNFVLASLSLCALAGVAAAETSAPRFINLQGGDMLSSNVVGLD
                     VYNDQKNDLGKIQDIVFNGRAVKGYILSVGGFLGVGTRYVAVDPSAVTVQYDAAAKKW
                     RASMNAAKDQLKAAPEFKYQGQWQASKS"
     gene            complement(8523..>8974)
                     /locus_tag="VKU03_13580"
     CDS             complement(8523..>8974)
                     /locus_tag="VKU03_13580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="EmrB/QacA family drug resistance transporter"
                     /protein_id="HLJ72345.1"
                     /translation="AQMAALPMLFLTITSFSYVGLPPGKSGQAAALINVARNLGGSIG
                     VSAAQTLLAQREQFHQSRLAENLYSSSLAYNHTLMQAASFFTGQGTSAEDARHQAIAW
                     IGRSLTTQAAYLSYIDVFAALSLFAAALIPIAFLLQKVDLGSAPRGH"
BASE COUNT         1532 a         2883 c         3047 g         1512 t
ORIGIN      
        1 gccgtttcgg gcagcaaaac gtcttggtag ccaataagcc acgccggacg atcgcagtca
       61 cccgaagggc cggaacgaag tgaaggaccc gccatagcgc cgcacgatcg ggcgttcgtt
      121 tgcgcgtaac tcgggaggcg ctatggcggg ttgacggcga tcggccggcg tggcgccgcg
      181 gcgctgaaag gttacgtctc agacgccgtg acaacctcag actttccccg gacagccctg
      241 cgcgaagcag gggagaggga ccatgcgaag catggtggag gggttccggt ctatcaggat
      301 aatcccaacc accgttgccc cctccaccgc gctgacgcgc ggtccccctc ccccgtcttc
      361 gacgggggag gatggccgta cgtcagagat cgccgcgtcg cgccagcgcc ttgatccgat
      421 cggcggtgcg gcaggcgatc gcttggatgg tcagcgacgg attgacgccg ccgacggtcg
      481 gaaacaccga gccgtcgcaa atccagagat tgggaatgtc ccacgagcgg caatcggcgt
      541 tgacgacgct ggtcgccgga tcgtcgccca ttcgcgccgt gccgttgaga tggcaagtat
      601 cgtcggtttc gtcccaaatg tcggtcgcgc ccgccgcctg caacgcttga cgcatgaagc
      661 cgagcgcgtg cttgatcatc tgcttgtcgt tgtcgcagta cgaataggtg acgcgcggga
      721 tcggcagccc gtattggtcc ttctcgtcgg ccagcgtcac gcggttgcgc tcctgcggca
      781 taaattcgcc gaccattttg agcccgacct gatgattgta tttctccatc tcgtcgatca
      841 ggcgctggcc ccagaggcca cgcttgctcg attcgacgcc ggaccagacg agcggcaggg
      901 gaccttggct catataggcg tagccgccga aaaaatcctt gccctcgtcc tggtagttcc
      961 agtgctcggt cagcgcgagc gagggcggcc ctttatagga gcggatctcc cgctccatca
     1021 ggccccatac ggcttggttg gcctggacca tcagattctt gccgaccagg ccggaactgt
     1081 tggcgagtcc gtcggggaat agctcgctcg ccgagttcaa aagcagccgc ggcgtctcga
     1141 tcgaatagca cgcgacgacg acattgcgcg cgcgctggaa gcgccagccg tcctggcgat
     1201 gatagtggac gccggtcgct cggccggcct catcgtgctc gatccatccg gccatcgaca
     1261 agtcgcggat ctccgcgccg gcggcgagcg cgcgcggcag ccaagtgagg agcacgctct
     1321 gcttggcgtt ggtcgagcag ccgatcacgc acatgccgcg atagacgcaa gggtgggcga
     1381 ggccgcgcgg cgccgagatt gtcgcaagcg gcgtcggcga ccaagcgatg cccatcgctt
     1441 cggcgccttc ggcaagaacg agggccgcgg cattgagctc gtgcggccgg tacggatagc
     1501 gcggacgccg gggcccccag ggataggtca ccggcccgga aattttcagc gtctgctcga
     1561 cttcgcggta atagctccac atttcccgcc aatcgagcgg ccagtcggcg ccatagccga
     1621 gcttgctgcg cgccttgaac cattccggcc ggaagcgcaa cgacaccatg gcgaaatgca
     1681 cggtcgaacc gcccaccgac ttgcccgaat tgttggcgcc gagttgaagc ggattttcgc
     1741 cgtcgacgat ccgctcgtct gtccaataga gcttcgcctg atgggtctcg tcggaagcaa
     1801 aatcttcgag cggccgccaa tacggtccgg cgtccaaagc gacgaccgaa aatccatatt
     1861 cggcgagctt gcaggcgagc gttccaccgc ccgcgcccgt gccgacaatg cagaaatcga
     1921 catcttcgtc ctcggaatag cagcgcatcg ggacccagcc gccaggccgg aacacgtccg
     1981 gcgcacgccc gccgatcgcg cgaggctggc tttgagggtc atccgacacg ggcattctcc
     2041 cgccgcgcct tctcttcgcg gcccggccgc gcttccgacg cctcccacgg atcgcggcga
     2101 tcgaaattca tccgcacata gccgcgcggg ctggccggcc cgccgaagcc gatttcgctc
     2161 cacgaggaag gatggccgta ataggcgctg acaatgtcgt gcagcaggcg cttctcaaaa
     2221 aaaagggcag gcggcatatc gccccaggct tcgtcatgcg cctcgccttt ttgtactttg
     2281 gcgagaatcg cctcctgctc cttgatcgtc aattcatgga agcgccggcc gtcgcgaacg
     2341 gcctcggcgt cgagcgccgc gaggccccgc cgccaggctt cgcgcatcga ggggaggcgc
     2401 gagtcccggt agccgtcggt gcggttctcg aaaagttttt catcgaccat cgcggcgatc
     2461 ggcgcccggt tcttgcgctc cggcggctgc ggaacgatgc gttcgcacag cgcgtgcatg
     2521 gtcggccatt cggcgtcgct gaaaaaccga tgctcgtcgg cggcgagcgc caggcgcttg
     2581 tcgatcaccg cccgggtctt gtcgttccag gacggcgaat tgcgcttgtt gagtacgtcg
     2641 tagccgggat aacggtcagg catcggcttc gttttcgatg agcaaaaggg cggcgaggcc
     2701 ggccaaagcc agcccggcga agctcggcgg cgccggcaga ggcgggccgt tgaggacgtt
     2761 ctggctccag ttgcgccagc cgcccatatt tcgcgacacg ccgtaaacgt ggaagccgga
     2821 gccgatgaag ccgagaagcg cggtcaaagc cagccaccag cgcgcgaggc gccgcggtcc
     2881 ctgtcgctgc agggccgagg cgccgagcag cgccgaagca acgggcggcg cgctcagcgg
     2941 caggatcatt gcggggttct ggaacgcgcc gcgaaaatgg agcaggccgg cttcgccgac
     3001 cgcgccggct atcccggtgg cgctgatcaa ggccagcaac cgcccgagcg gcaggccgag
     3061 gcgtttcatg ggccgacgag agcgcttgcg aacccgttcc gcgccgcggc caagcaggcc
     3121 ggccatgacc aaagcgccgg gcgcgccgat cggcgcagcg tagaaaaaat tgagccagga
     3181 gaagccgccc ggccgcttgg cgatgttgta aatgtgaaag ccgaggccgg cgacgccgac
     3241 cgcggtcgcc gcgccgtaaa tggcgtcgcg ccacagccga ggctgcgggc gctcctccgt
     3301 cgaggcggcg aggctcgcgg cgagcgccaa agccgaggcg acgagcggcg catacatggc
     3361 gcgattttcg aacgagccgc gataatgctc gatcgcgctg tcggcgagaa ccgaaagcgc
     3421 gaggatcgcc gcgccctggt tgagctggcg cgccgcggcc gcgtccgaac cgcgccggcg
     3481 aaagatcgcg ggcagccgcg ccgcttgagg cagggccgga agcgcgcgca gcgctttgcc
     3541 ggaggtcggc gtgagcgctt cgagaatatc ggtgggccgg cggatcatcg gatcgctcca
     3601 tcgatgcgga attgctcggc gtcctgcgcc ttgaaatcga gcccgaggcc gggccgcgaa
     3661 agatcgggcc tgatcgcgcc atctttggcg acgggcgcgc cgtcgaacag catctgctcg
     3721 atgcggacgt ggtcgtggaa ccattcgaga tgacgcaagc gcggcgccgc gcaggcggcg
     3781 tggcggtgca gcgccggcgc gcaatggccg gaaagatcga tgtgatgggc ttggcaaagc
     3841 gcggcgactt cgaggaaccc ggagacgccg ccgcatcgcg tcgcatccgc ttgcaacacg
     3901 tcgacggcgc cggcttcgag caggtgacgg gcgtcgtcca gcgtgaagat atattcgccg
     3961 gccgcgacct cggtctgagc cggcgtcctg tcgcgcacga ggcgcagtcc ggccggatcg
     4021 tccgacgtga ccggctcctc gaaccagcgt atgtcgaggt cgttgcaggc ttcgatgaag
     4081 cgcagcgcct gcttgaccga gaaggcgccg ttcgcgtcga cgaaaagctg cgtgtcgccg
     4141 atggcggatt tggcggcgcg ggcgcggcgc ggatcacgct cgggctccga gccgattttc
     4201 atcttgacga agcggcatcc gtctcgctcc gcccaacccg caagctgctc cttgagcctt
     4261 tcgtcggagt aggaggtaaa gccgccgctg ccgtaaatcg gcacatggtc gcgacagcgg
     4321 ccgagcagcc gggccaatgg cagatcgagc agtcgcgcct taaggtccca caaggccgcg
     4381 tccaccgcgg agatcgcgca tgccgcaacg ccggagcgtc cgagattgcg caccttgcgc
     4441 cgcattgcgg ccaaggcgcc gggaatatcg aaacattcgc gtccttgaag aacctcagcg
     4501 agctgctccc gcgccagcac ggcggcggaa gcgccagcat aggtatagcc gaggccggat
     4561 tgtccggcgg cttcgattcg cgccacaaca agcgtcgtgg cgctccattc aaacgtgccg
     4621 tcggcctcgg gcgcatcggt cggaatgcga taagcggcgg cgctcagtcc ggcgatcggc
     4681 gcgtcggggc gggccgccgt cattttttat cgccgccggg cagcactgcg ctgaggactt
     4741 gccttgccgt ctcccggatg acgctgcctt cgcgcggatc tcccttgatc agcgtctcgg
     4801 taaatttctt cgcctgttcg agggtaatgt gcggcgacag cggcggcact tccggatcgg
     4861 tcttgacctc cagcaggacc ggccggcgcg aggccaaagc ctcgtcccag gccgcgccaa
     4921 gcttttcggg gtcgtcgaca tagatcccct tgaggccgat caattcggcg aagcgatggt
     4981 acggaacgtt ggggatttcc tggctggcgt tgaatttcgg atcgccgttc atcacccgct
     5041 gctcccaggt gacctggttg agatcctcgt tgttgaagac gcagacgatc cattgccggt
     5101 tcgaccattt gcgccagtat ttggcgacgg tgatcagttc ggccatgttg ttcatctgca
     5161 tcgctccgtc gccgaccagc gcgacgaccg gccggtcggg atgggcgaat ttcgcggcga
     5221 tcgcataagg cacggccgcg cccatcgagg cgaggccgcc ggacagcgag gccatcatgc
     5281 cgcgcttgat cttgaggtcg cgcgcccacc aattggcgca ggagccggaa tcgctggtca
     5341 agatcacgcc gtcgggcagg cgcggcgaca actcccaggt gacgcgctgc ggattgaccg
     5401 gattggccgg ctgcatggcc cggtcttcga gcgtcttcca ccactccttg acattcttct
     5461 cgatctcgtc gcgccagccg cgatcctgct tgtggacaac catgggcagg agcaggcgca
     5521 acgtctccgc cgcgtcgccg acgagattga cctccattgg atagcgcagc gacagcatgt
     5581 ccggcgcgat gtcgatctgg acgccgcgcg cctggccttc ttcgggaagg aattcggaat
     5641 agggaaagcc ggagccgatc atcagcagcg tatcgcagcc catcatcatg tcgtagctcg
     5701 gcttcgtgcc gagcaggccg atcgagccgg tcacgaatgg aaggtcgtcg ggcagcgcgg
     5761 ctttgccgag cagcgccttg gcgacgccgg cgccgagctt gtcggcgatc gcgatgacct
     5821 cgtccgtcgc gcgcagcgcg ccggctccga ccaggatcgc gaccttgctg ccggcgttca
     5881 aaacggcggc ggcgcgccgc aaatccgttt cgtaaggctc gattttgggc cgcgcaaagc
     5941 cgacgccgga atgaaccgag ccgtgcttgc gcggcggctc acggtaggtc tcctcctgga
     6001 gatcgttcgg caggatgatc gcggtcaccc gccgctcggc gatcgcaatg cggatcgcgc
     6061 ggtcgaccag atggcgcact tgcggcgggg aggaagcttg ttgcacgaag gcgccggcga
     6121 cgtccttgaa caagctggcg aggtcgactt cctgctgata atggccgccc atcgcgttgc
     6181 gcgcctgctg gccgacaatg gcgagaaccg gcatgtgatc gagccgcgcg tcgtaaaggc
     6241 cggtgatcag atgggaagcg ccaggccctg aggtggcgat gcagacgccg agctcgccgg
     6301 tgaatttggc gtaggcgctg gccatgaagg ccgccatctc ctcatggcgc gcttggatga
     6361 attcgatctt gcccttggcg cgattgagcg cgccgaagac gccgtttatc ccgtcgccgg
     6421 gatagccgaa cattttgcgc acgccccaag cgtgcaaccg ctctacgata aaatcgccga
     6481 ctgtctgcgc catgacgaat ttccccgtgc tcgcctgatc cagccagcca ggcgcggaag
     6541 ctccggccgg aaaacgattc ggcgggcggg gagttccgaa ctaaaacgcc gcgccggagg
     6601 gagggtttcg cttcccgccg ccgccgcgcc acggagaagc cgcctttcgg cgcccgtgcg
     6661 aacctcactc ggcccgcgcc agctcgccga gcagcgattc ggcgcgggtg cggaacgccg
     6721 ccggcttggc gcgcttcatc gtcttggcca atgcgccgcg gcggaagctc ttctcgacga
     6781 tggcgtggac atagcttttg cgcgcaatgg ccggcgtgtt ggcgagctgc tcggccacca
     6841 ggcgcatgac cgaggacaat tggcggcgtt ggccggtctc gctggcggcg ggcgcgacgg
     6901 cggccagttc ctcggcggcc aaagcgctgg cgttcgtcat cctgaaatcc ttggcgctga
     6961 cggcggcgcc ggagacccgc tgcaggtagg cgttgacgtc ggcggcggtg agcgcgcgcg
     7021 gccggccttc cttgtcgaga aattggagca agcgccgtcc cggaatcgcc gcgagccggc
     7081 ggagcgcgcg cgccagagcc gggtcggcaa tttcgcgctc gatggttctg ccgcctttgc
     7141 ctctaaattg cagcttgatc tggtcgcggc cgatcgcggc gtgctgtttc agcagggtcg
     7201 ccgcgccgcg gccgccgtcg gcgcgcgcat aatcctcgca gccgacgcgg atgcagctct
     7261 cgtcgatcat cgcgacggcg caagccgcag ccttctcgca ggaaagctct ttcgccgcga
     7321 catcgcgttt gaccgccgcg cgtatctttg gcagggccgc gacgagctcc gccatgcggc
     7381 cgaccttgcg cttctcgcgc acgtccgtcc aatcggggtg gtagcggtat tgccatcggc
     7441 cggcggcgtc gcggccgatc gcttgaagat gcgcgcgctc gtcgaaagca atgcggacgt
     7501 cgctataggc cggcgggatg gccattttgc ggatgcgcgc gagcgtcgcg ggatccttga
     7561 tgagccggcc ggccgaatcg aaataggcga agccgcgccc gacgcgccgc cgctcgatat
     7621 cgagctcgga gacggcgatc tttcggagtc gggcgctcat ccgcgagcct caggcgaccg
     7681 ccggcggcgc ttcgggcggg agttcaggcg gaagcgtcgc cggaatctcc tcaggctcgg
     7741 gggcgggaac gacgtcgggg cccggttgcg gaacgccggc cggcaaatcc ggtttcggcg
     7801 tttcctgcgg cgtcggcgga tgcgccgggt tcggcgtcac atccggcggc gggcgcggaa
     7861 taggttgttt cggattcgcc ggcattttac ggacctgttc tgatcaaacc tcagaaaaga
     7921 caatgtccca cgcgatgctt tgttccggcg gcgagtctgc cggagcgccc gccgttgcca
     7981 aggcgcgccg ccaagagtca cccgagaggc gccaaggtac gagggcgcgg cggcctgcgg
     8041 aactttcgtc cgttcggccg cgttgctgcg cgacggcgcg agttgtgggg aggattgcct
     8101 gatgcgcaat ttcgtcttgg cgagcttatc gctttgcgcg ctggccggcg tcgcggccgc
     8161 ggagacgagc gcgcctcgct tcatcaatct tcaaggcggc gacatgctca gctccaatgt
     8221 cgtcggactg gatgtctaca acgatcagaa aaacgatctc ggcaagatcc aggatatcgt
     8281 cttcaatggt cgcgcggtga agggctacat cctctcagtc ggcggttttc tcggcgtcgg
     8341 cacgcgatat gtcgccgtcg atccgagcgc cgtgaccgtc caatacgacg ccgccgccaa
     8401 gaaatggcgc gccagcatga atgccgcaaa ggaccagctc aaagccgcgc ctgaattcaa
     8461 gtatcaaggc cagtggcagg cgagcaaaag ctgatctcga aaagagagcg cggcgggcgc
     8521 gctcagtgtc ctctcggggc cgatccaaga tcgacctttt gcagcaggaa ggcgatcgga
     8581 atcaacgcgg cggcgaaaag cgacagagcc gcaaagacgt cgatatagga aaggtaggcc
     8641 gcctgggtcg tcaggctccg cccgatccag gcgatcgcct gatggcgcgc atcctcggcg
     8701 ctcgtccctt gtccggtgaa gaaggacgcg gcttgcatga gggtatgatt ataggcgagc
     8761 gatgaactgt agaggttttc ggcgaggcgc gactgatgga attgctcgcg ctgggcgagc
     8821 agggtctgcg ccgccgagac gccgatgctg ccgccgaggt tgcgagcgac attgatcagc
     8881 gccgcggcct ggccggactt tcccggcggc agcccgacat aagagaagct ggtgatcgtc
     8941 aggaacagca tcggcaaggc cgccatttgc gcca
//
LOCUS       DATGJW010000452         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_192604, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000452 DATGJW010000000
VERSION     DATGJW010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_192604"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..213)
                     /locus_tag="VKU03_13585"
     CDS             complement(<1..213)
                     /locus_tag="VKU03_13585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72346.1"
                     /translation="MSMNRVAAGGDIAIQEGFEISSSNFCCRDSEKAGGPQGVFETTQ
                     VAMTQIPNAALPSTRRRVKVKPLRGRS"
     gene            complement(254..1255)
                     /locus_tag="VKU03_13590"
     CDS             complement(254..1255)
                     /locus_tag="VKU03_13590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008971179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72347.1"
                     /translation="MTSRPVVIVTGASAGIGRAIAQRFASADCRVGLIARDEAALEAV
                     RAEIVERGGTAAVAAADVADAASVFAAADRLEQALGPVDIWINDAMETVFSRVADITP
                     EEFRRVTEVTYLGFVHGTMAALKSMRPRGRGQIIQMGSALAYRGIPLQAAYCGAKHAI
                     RGFTNSLRTELIGEGSRIKVAIIELPAVNTPQFDWARTHMARTPRPMGAPIEPEVVAD
                     AVYRAAQGAWREYWLGLPTLLTILGNTVLPGYLDRYLARTGIKGQQTSTPVAADRQDN
                     LDRAVPALHRTRGSFSAEAGASAPLVPGEVARLGVVAAGAAVFFGLGALFGALRRSR"
     gene            1796..>2187
                     /locus_tag="VKU03_13595"
     CDS             1796..>2187
                     /locus_tag="VKU03_13595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015667071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing protein"
                     /protein_id="HLJ72348.1"
                     /translation="MRVGDFSVRMAGDHAGIMGKVADAFNEIVAANQRMARQLERVGQ
                     VVGREGKTRQRVRFGLSSGAWGEMEASVNTLIDDLLWPTTEVTRAIAAVAQGDLLQTV
                     RLDVDGRPLKGEFLRAAEIVNTMIKQLSV"
BASE COUNT          394 a          710 c          730 g          353 t
ORIGIN      
        1 ggagcgaccg cgaagcggct tgaccttgac gcgcctccgc gtcgatggca gcgcggcgtt
       61 tgggatttgc gtcattgcca cctgtgtagt ttcgaaaaca ccttgagggc cgcctgcttt
      121 ttctgagtct cgacaacaga aattcgatga agaaatctcg aaaccttctt gtattgcgat
      181 atcgccgcct gcagcaactc gattcatgct cattttcgcc ggacagccct gcttcgcgga
      241 ggaggaaggt acgtcagcgg cttcgccgca acgcgccgaa cagcgcgccc aggccaaaaa
      301 agacggctgc gccggcggcg accacgccga gccgcgcaac ctcgccaggc acaagcggcg
      361 cggaagcgcc ggcttcggcg ctgaacgagc cgcgcgtccg atgcaaagcg ggcacagccc
      421 gatcgagatt gtcctgacgg tcggcggcga ccggcgtcga cgtctgctga cccttgatcc
      481 ccgtgcgggc cagataacgg tcgagatagc ccggcaggac cgtgttgccg aggatcgtca
      541 gcaaagtggg caagcccagc cagtattccc gccaagcgcc ctgcgccgcc cggtagaccg
      601 cgtcggcgac cacttcgggc tcgatcggcg cgcccatcgg ccgcggggtc cgcgccatat
      661 gggtgcgcgc ccaatcgaat tgcggtgtgt tgaccgccgg caactcgatg atcgcgacct
      721 tgatccgcga gccttcgccg atcagttcgg tccgcagcga attggtgaag ccgcggatgg
      781 cgtgtttcgc tccgcaatag gcggcctgaa gcggaatgcc gcgataggcg agcgccgagc
      841 ccatctggat gatctggccg cgcccgcgcg gccgcatcga tttcagcgcg gccatggtcc
      901 catggacaaa gcccagatag gtgacctcgg tgacgcggcg aaattcctcc ggcgtaatgt
      961 ccgcgacacg ggagaagacg gtctccatcg cgtcgttgat ccagatatcg accgggccga
     1021 gcgcttgttc gaggcgatcg gccgcggcga aaaccgacgc ggcgtcggcg acatcggccg
     1081 cggcgacggc cgccgtcccg ccgcgctcga cgatttcggc ccgcacggct tcgagcgcgg
     1141 cttcatcgcg cgcgatcagg ccgacgcggc aatcggccga cgcgaagcgc tgcgcgatgg
     1201 cgcgcccgat ccccgccgat gcgccggtga cgatgaccac aggtcgggat gtcatgagcg
     1261 attgcttcgt tgcgatcttc ttttgcggaa acgtggcgct ttccgccaag ttcctgggcg
     1321 gcggggcgcg aacggcgcct tgcgccgaat ccaccagccc gatttgcaca atcaacaaac
     1381 cggccgttcg ccctgattaa acacgcaaaa aagatacgcc cgctccgccg aagcgggaac
     1441 ttttcaggcg caatcggcgt tcgggtgggc cactggtagg gcgcgcgttg gggactctca
     1501 ttgcaagcta acgaaacagc ggccggtttg cgccggggcg gccggcaagg ctgcgcctgc
     1561 attcgggccg acgcggcgct atggaaacgc cccgcaaggc catgggattg ctcgattgtt
     1621 gggcttctct ggctggaaat tcgctccgag cattcgatag ggagacacga tgagcgacca
     1681 aagtctcgct gagcgcggcg ccaagagcgg agcggccgcc caggcgcgcc ctgaaaagcg
     1741 gttgaacggc ggctccccga gcgaattgcg ggagcttttg aacgcgctcc aggcgatgcg
     1801 ggtcggcgat ttttccgtcc gcatggccgg ggaccatgcc gggatcatgg gcaaggtcgc
     1861 cgacgcgttc aacgagatcg tggcggccaa tcagcggatg gccaggcagc tcgaacgcgt
     1921 cggccaggtc gtcggccggg aaggcaaaac ccgccagcgc gtgcgcttcg gcctgtccag
     1981 cggcgcctgg ggggaaatgg aggcctcggt caacaccctg atcgacgacc tgctttggcc
     2041 gaccacggaa gtgacccgcg ccatcgccgc cgtggcgcaa ggcgacctgt tgcagaccgt
     2101 ccggctcgat gtcgacggcc ggccgctcaa gggcgaattc ctgcgcgccg ccgagatcgt
     2161 caacacgatg atcaagcagc tcagcgt
//
LOCUS       DATGJW010000453         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1226782, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000453 DATGJW010000000
VERSION     DATGJW010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1226782"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1942
                     /locus_tag="VKU03_13600"
     CDS             137..1942
                     /locus_tag="VKU03_13600"
                     /EC_number="2.7.7.65"
                     /EC_number="3.1.4.52"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0052621 - diguanylate cyclase activity
                     [Evidence IEA];
                     GO_function: GO:0071111 - cyclic-guanylate-specific
                     phosphodiesterase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional diguanylate
                     cyclase/phosphodiesterase"
                     /protein_id="HLJ72349.1"
                     /translation="MTNQDVSAGRRSLLRKPLIETLSLRGPAKAATPVASAPAAPAKP
                     VEHEPRDILNSIRAVVYNWDLTTDRICWGANVKDVLGKFSDSALECGDSFADLVADNS
                     ESSRYHAIVHGGGQASEEGAPYRAQYGLLTEGRDVIWVEDFGRWFADASGGPGRAHGV
                     VRIIADPSQRDDRGEIAKRDPLTGAVGRGRLIEHINAQCAEIARRGAPFAVLIVGIDR
                     LAALNERFGYETVDGAIVAVSRRLHDCVRATDIVARYLGGRFALVLDTCDAEQCALLG
                     RRILQAAKEPIRIAGREIRISLRIGAALAPKHGRSAQALLQRAEEAYDFSCRSRGGVM
                     VFTPALAARGAEARLSSMSDEIVAALNERRVVLAYQPVAPARGGRQRFYEALLRIREP
                     GGAFLGPSAILPVAERVGLIAQLDQRVLELALKRMSIEPDLRLSINASPATMIEPDWI
                     AHFANALAAHPGVARRLILEVTESQAIADLERTARLFEEIRRLGVKVAMDDFGAGHTS
                     FRNLRRLGVDIVKIDGAFVQNIARSADDRFFVRTLLELARNLNVETVAEWVEDAEAAR
                     ILIDWGVDYLQGHHFGAAEPQDEALAAPMLAAAIG"
BASE COUNT          338 a          740 c          712 g          373 t
ORIGIN      
        1 acgggggagg ataatccccg caatttggcg cgaattttac cgttcgttaa tcgctcgctt
       61 ccattgttcg cgtttgtcgc catgacgcgc catggaccgc ttcgtgcgcc tctcgcggca
      121 ataggagcgg gccgcattga ccaaccagga cgtctcggcc ggcaggcgat cgctcctccg
      181 caagccgctt atcgaaacgc tgagcctgcg agggccggcg aaagccgcga cgcccgtcgc
      241 ctctgcgccc gccgcgccgg caaagccggt tgaacatgag ccgcgcgaca ttctcaattc
      301 gattcgcgcc gtcgtttata actgggattt gacgaccgac cgcatctgct ggggcgccaa
      361 cgtcaaggac gtgctcggca aattttcgga cagcgcgctg gaatgcggcg attccttcgc
      421 cgatctggtc gctgacaatt cggagagctc gcgctaccac gcgatcgtcc atggtggcgg
      481 acaagccagc gaggaaggcg cgccctaccg ggcgcaatac ggcctcctca ccgagggacg
      541 cgacgtcatc tgggtcgagg atttcggccg ctggttcgcc gacgcttccg gcggaccggg
      601 gcgcgcccat ggcgtggtgc gcatcatcgc cgatccgagc cagcgcgacg accgcggcga
      661 gatcgccaag cgcgaccctt tgaccggcgc ggtcggccgc ggccgcctga tcgagcatat
      721 caatgcgcaa tgcgccgaaa ttgcgcggcg cggcgcgcca ttcgccgtgc tgattgtcgg
      781 catcgaccgc ttggccgcgc tcaacgagcg tttcggctac gagaccgtcg acggggcgat
      841 cgtcgccgtc tcccgccgtc tccacgattg cgtgcgcgcc accgatatcg tcgcgcgcta
      901 tctcggcggg cgctttgcgc tggtcctcga tacgtgcgac gccgagcaat gcgcgcttct
      961 cggccgccgc attctgcaag cggcgaagga gccgatccgc atcgccggac gcgagatcag
     1021 gatttcgctg cgcatcggcg cggcgctggc gccgaagcac ggtcgcagcg cccaggcgct
     1081 gctgcagcgc gccgaggagg cgtacgattt ttcctgccgc agtcgcggcg gcgtcatggt
     1141 gtttacgccg gctctcgcgg cgcgcggcgc cgaggcgcgc ttaagctcga tgagcgacga
     1201 gatcgtcgcg gcgctgaacg aaaggcgcgt ggtcctcgcc tatcagcccg tcgccccggc
     1261 ccgcggcggc cgccagcgct tttatgaggc cctgctgcgc attcgcgagc cgggcggcgc
     1321 ctttctcggc ccgtcggcga tcctcccggt cgcggagcgc gtcggcttga tcgcccaact
     1381 cgaccagcgc gtgctcgaac tcgcgttgaa acgcatgagc atcgagcccg acctgcgcct
     1441 ttcgatcaac gcctcgcccg cgacgatgat cgagcccgat tggatcgccc atttcgccaa
     1501 cgccctcgcc gcccatcccg gcgtcgcgcg gcgtctgatc ctcgaagtca ccgagagcca
     1561 ggcgatcgcc gatctcgagc ggacggcgcg gctcttcgag gagatccggc gtctcggcgt
     1621 gaaagtcgcc atggacgatt tcggcgccgg ccatacgtcg ttccgcaatt tgcgccggct
     1681 cggcgtcgac atcgtgaaga tcgacggcgc gttcgtgcaa aacatagccc gctccgccga
     1741 cgaccgcttc ttcgtgcgga cgcttctgga gctcgcgcgc aatctgaatg tcgagaccgt
     1801 cgccgaatgg gtcgaggacg ccgaagcggc ccgcatcctg atcgactggg gcgtcgacta
     1861 cctgcaaggc caccatttcg gcgcggccga gccgcaggac gaggcgcttg cggcgccgat
     1921 gctcgccgcg gcgatcggat agagcccgaa cgccgcgatc ggcgctcaaa agcagccgct
     1981 gcgccttacg acttgccggc cagcgagtcg agccgcttct gcacgtccga taattgcttc
     2041 ttcaattctt caaggtcgga attggcggag gccttttccg gcgccggcgc ggcgccggcc
     2101 tggggggcgg cgacgaaggg cgtgaacata ttcagcgcct gcgagaaagc ggcgagattt
     2161 ttg
//
LOCUS       DATGJW010000454         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1020756, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000454 DATGJW010000000
VERSION     DATGJW010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1020756"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1161
                     /locus_tag="VKU03_13605"
     CDS             <1..1161
                     /locus_tag="VKU03_13605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="integrase"
                     /protein_id="HLJ72350.1"
                     /translation="PGRKVMRRPNGRIDVYWVASPEAVRAGYEAKTRPLKGDPGERQA
                     PPEIAALCRLYWAEMLEFLAGRRAPAGSALVGTIAWLVERYERDEESAYHRIRPATKQ
                     GYDKALAIIRAEVGERRIDAVTAKDLWRWFKAWGRADDAGTLHNPRRAYGCIQILRLI
                     VKHGKGLRDQACRELSEILSETEFPVPRARKQAMTAAHVNAFIPKAHELGYPSVAFAV
                     ALQFSCSLRQKDVIGEWLNDQWGWGLRWGEHISPDLRLEKPTSKSGGKEVAEFDLRLL
                     PIVMREIERIPSTSRIGVVIVDERTRKPYRQREFARRFREIARAAGIPDEIWNMDARA
                     GAITEAYDKGATEVDAMSLATHRQLATSLRYKRGKIKSTSRVSVLRFGGKSE"
     gene            1458..>3062
                     /locus_tag="VKU03_13610"
     CDS             1458..>3062
                     /locus_tag="VKU03_13610"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72351.1"
                     /translation="MEKRESTVVTIAPVRMCGTTIRVYRYGLAPPLDWDDECDAEMAR
                     LDRFYNALVEIEEAAQAEYRRLSSSDETALLETRIAAAEEAKDWGAAKALRAALKEIR
                     AALRKANAAAIDAAEEKRKADAKAARQNCGAYWSSYNAVIRSVELARQKAIKEGAAFG
                     KRTHEPGKGDWRMTVQIQGGASVADVLGGKNSQLRIAAPAHFGALRDRPAGMSRKACR
                     HGRVTMVVHNTGGLRRVTWPLMMHRPIPPEAIIVGAEIVKRRRLGSRWDDWHLCVTVR
                     EPAPAPHESPDCAGVNIGWRRLSVERGLVVDGAGLRIAMIWDGATLNHVILPEEIISA
                     AWRCDELTSAIDKRVDAATARIFKDSPDHPVARQLGDTFVESGRLRVRDLWTFANAMT
                     PAPDWLLAMLRACSRDRRERAGLLRRMARRRRDIYRVAAKTIAENYGRIAICAVDWAK
                     LARLRESGKDNPLPPPARGYRKIAAPGEFEAELRRAMKARGATIMDIKDSVSFLCHAC
                     GKEHAPSERSAAHHTCPSCGATWDQDR"
BASE COUNT          593 a          920 c         1052 g          497 t
ORIGIN      
        1 ccagggcgta aggtgatgcg ccgcccaaat ggccgcatcg atgtctattg ggtggcgtcg
       61 ccagaggccg tcagggcggg ctacgaggcc aagacgcggc ctttgaaagg cgatccgggg
      121 gagcggcaag ccccacctga aatcgccgct ctgtgccgcc tctattgggc ggaaatgctc
      181 gaatttctgg cgggtcggcg agcgccggcc gggtcggctc tagtcgggac gatcgcgtgg
      241 ctggttgagc gatacgaacg agacgaggag agcgcctatc atcgcatccg gccggcgacg
      301 aagcaaggct atgacaaagc cttggcgatc atccgggcgg aggtcggcga acgccggatc
      361 gacgccgtga cggcgaaaga cctgtggcgg tggttcaagg cgtggggccg cgccgatgac
      421 gccgggacgc ttcacaatcc gcgccgcgcc tacggctgca ttcagatctt gcggctcatc
      481 gtcaagcacg gcaaggggct tcgggaccaa gcctgccgcg agctttcgga aatcctgtcc
      541 gaaaccgagt tcccggtccc gagggcgcgc aagcaggcta tgacggccgc gcatgtgaac
      601 gcctttattc cgaaggcgca cgagctcggt tatccaagcg tggccttcgc cgtcgcgctt
      661 caattctcgt gctcgctccg ccagaaagac gttatcggcg agtggctgaa cgatcaatgg
      721 ggctggggcc tgcgttgggg cgagcatatc tcgccggacc tgagactcga aaagccgacc
      781 agcaagagcg gcgggaagga ggtcgccgag ttcgaccttc gcctcttgcc gatcgtcatg
      841 cgggagatcg agcgcattcc gtcgacaagc aggatcggcg tcgtcattgt cgacgagcgg
      901 acaaggaagc cctatcgtca gcgtgagttc gcccggcgtt ttcgcgagat cgcccgcgcc
      961 gccggcattc cagacgaaat ctggaacatg gacgcgcgag ccggcgcgat caccgaagcg
     1021 tacgacaagg gcgcaacaga ggtcgacgcc atgagcctcg ccacgcatcg ccagcttgcg
     1081 acgagccttc gctataagcg aggcaagatc aagagcacga gccgggtaag cgtgctgaga
     1141 tttggaggta agagcgaatg agcaaaccca aaattatgtt cgttcttgat tccagcggtt
     1201 gcgttttctg cgatgtcgac atgccggcgc agggcggctg gcacatgctt aagagcggtc
     1261 ccattaagtg cactgtcgca ttagcgaacg cagcaaaaac aggacggcgt aacagcgcac
     1321 gtaacaaatc taagattaag aataacaaaa agcgtctaag tgttggtgcg gacggcggga
     1381 tttgaacccg catgactggt caagtcgagg gattttaagg ctgtcggcga gcctttgaaa
     1441 gattagggga cgttacgatg gaaaagcgcg aaagcaccgt ggttacgata gcgcccgtcc
     1501 gtatgtgcgg cacgaccatc cgcgtctatc gctacggcct cgccccgccg ctcgattggg
     1561 acgacgagtg cgacgccgaa atggcgcggc tcgatcgctt ttataacgct ctggtcgaga
     1621 tcgaggaggc ggcgcaggcg gaatatcgcc gcctatcctc atccgacgag acggcgctcc
     1681 tcgaaacccg cattgccgcg gccgaagagg cgaaggactg gggcgcggcc aaggcgctgc
     1741 gggcggctct gaaagaaatc cgcgccgcgc tgcgcaaggc caatgcggcg gcgatcgacg
     1801 ccgcagagga aaagcgcaaa gcggacgcca aggcggcgcg gcagaactgc ggcgcttatt
     1861 ggagttcgta taacgccgtg atccgctccg tggagctcgc gcggcaaaag gcgatcaagg
     1921 aaggcgcggc attcggcaag cggacgcatg agcccggcaa gggcgactgg cggatgacgg
     1981 tgcaaattca gggcggcgcg agcgtcgccg acgtgctcgg cggcaagaac tcgcaattgc
     2041 ggatcgcggc gccggcgcat ttcggcgcgc ttcgcgatcg gccggcgggc atgtcgcgca
     2101 aagcctgccg ccatggccgc gtgacgatgg tcgtgcacaa caccggcggt ttgcgccgcg
     2161 tgacttggcc gctgatgatg caccggccga ttccgcccga agcgattatt gtcggcgccg
     2221 aaatcgtcaa gcggcgtcgc ctcggctcgc gctgggatga ttggcatttg tgcgtcacgg
     2281 tgcgcgagcc cgctcccgcg ccacacgaga gcccggattg cgccggcgtc aatatcggct
     2341 ggcggcggct cagcgtcgag cgcggcctcg ttgtcgacgg ggccggcctc agaatcgcga
     2401 tgatctggga cggcgcgacg ctgaaccatg ttattctgcc ggaggaaatt atctccgcgg
     2461 cgtggcgttg cgacgagctg acgagtgcga tcgacaagcg cgtcgacgcc gcgaccgcgc
     2521 gcatcttcaa ggattccccg gaccatcctg tcgcgcgcca gttgggcgac acgttcgtcg
     2581 agagcggtcg gctgcgggtg cgcgatcttt ggacattcgc caacgccatg acgccggcgc
     2641 cggactggct tctggccatg ctgcgggcgt gctcgcgcga tcgtcgcgag cgggccggct
     2701 tgctgcggcg gatggcgcgg cgcaggcgcg acatctatcg cgtcgccgca aagacgatcg
     2761 ccgagaatta cggacgcatc gcaatctgcg ccgtcgattg ggccaagctg gcgcggctgc
     2821 gcgagagcgg caaggacaat ccgcttccgc cgccagcgcg cggctatcgc aagatcgccg
     2881 cgccaggcga gttcgaggct gaactccggc gcgcaatgaa ggcgcgcggc gcgacaatca
     2941 tggacatcaa ggatagcgtc agcttccttt gccacgcctg cggcaaggag catgcgccga
     3001 gcgagcgcag cgcggcgcat catacttgtc cgtcctgcgg cgcgacctgg gaccaggacc
     3061 gc
//
LOCUS       DATGJW010000455        11360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_499004, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000455 DATGJW010000000
VERSION     DATGJW010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11360
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_499004"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            94..1368
                     /locus_tag="VKU03_13615"
     CDS             94..1368
                     /locus_tag="VKU03_13615"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ72352.1"
                     /translation="MRSSRKIARRTVLKGMLASGLAGVAGKNVAFAAPRAIKIGLVMP
                     QTGILAAFCEHMPFVLAQVKKVTGDKLTINGAVHPFEIVIKDSQSDPNRASEVAQELI
                     LNDKVDIVSTFATPETVNPVSDQCELNGVPCVSNDAPLEPYFFGRHGDPKKGWDWTYH
                     FFFSGDELVKAMIPYWNRLPTNKVIGALWPNDGDGIAQSKGFPPLFEKAGYKTVDPGR
                     FDMPASSYNAQIAAFKSAGCEIITGVVPPPEMTTFWNGCAQQGFKPKCCYMGKALEFP
                     SAIAPLGKRVEGLTTEVWWAATSPYSSSWTGQTSQQLADAYEAASGRQWSQPLGFRHS
                     LFEVIFDTLKRTHDLDKAASIRDALKTTKLNTIVGPVDFATGPLPNCSLTPLVVGQWT
                     KGKKYPFDITIVDNTLYPNVPVGGAPSVIHYA"
     gene            1401..2123
                     /locus_tag="VKU03_13620"
     CDS             1401..2123
                     /locus_tag="VKU03_13620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019860235.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72353.1"
                     /translation="MLELVELSRRFGALKVIDGLSLSVAEGDILGVLGPNGAGKTTLF
                     NLVLGVLAPDSGRIAFQGRDITRMRPWDRCRAGIGRTYQVPRPFAHMSVYENVLVAAV
                     HGGRSSVRASREWARQVVDLTGLGRRLDHRAGDLSLLDLKRLELAKALACKPKLLLLD
                     EIAGGLTDLECDQLLDIVRNARQSGATIVWIEHVIHALRRIASRLAVLHGGRIISAGA
                     PDEVLADPRVREVYLGVEAEAL"
     gene            2120..2824
                     /locus_tag="VKU03_13625"
     CDS             2120..2824
                     /locus_tag="VKU03_13625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011925322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72354.1"
                     /translation="MSDALLNIENLSVFYGEFRALFGVDIAVKAGEIVSMIGANGAGK
                     SSLLKAIVGQVGRIEGRIAFDGDDLARLSTPEIVARGVALVPEGRRLFPSLTVEENLT
                     IGQEIGRGGDLGFDEIYRYFPILWEKRRQKTRELSGGQQQMAALARALLANPRLLLCD
                     EISLGLAPVAIAALYQVIPTIRDRGVAILLVEQDITRALAVADRFYCLLGGRVNLSGL
                     PRETTRETVAHHYFGG"
     gene            2833..3699
                     /locus_tag="VKU03_13630"
     CDS             2833..3699
                     /locus_tag="VKU03_13630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007199961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ72355.1"
                     /translation="MAAIWINQIVQGVLLGGLYAMFAMGLSLSIGVMQFVNIAHGDLI
                     VLVSFLLLTLTSGLGLPPLLAAAITLPIAFVGGFLLQRLLLQRVLGKGVLPIILVTFG
                     LSIIIQNGLLSAYGADTRKVAGGAIETATIPIGGGVNIGVLPFIIFLSAIVLIFALDR
                     LLYHTRLGARIRAVSDDVATANLIGLKSARIYAVAMGVVGATIAISAAFMSVWTNFDP
                     SSGPSRLLVAFEAVVLGGLGSLWGTLVGGVALGVAQAIGAQFDAAWQILAGHILFLVI
                     FVIRPQGLFPRN"
     gene            3713..4750
                     /locus_tag="VKU03_13635"
     CDS             3713..4750
                     /locus_tag="VKU03_13635"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HLJ72356.1"
                     /translation="MNASGTWLIAIGRLALILGAGVLAAAPFLFAPAQLQILTQFLSL
                     LVIAIMWNLLAGYADIVSIGQHAFVGIGAYAFFAFAALAGVNPYLSLLLGGVTAFVFA
                     VPIMLVIFRLRAAYLAVGSWVVAETLMLGAGKLSAFGGGSGVSVPISVVKAFGAQAAA
                     RYATIYWLAFALAALALVSTYLLMRSKVGIGLTAMRDNEEGAGSVGVNLIALRVLCFL
                     WTAPFLGIAGGVITLQKLRVAPPASFSITDWTVYVIFIVVIGGIGSFEGPIIGAIVFF
                     LIREYLAEIGLWHFILLGVLSIAVILVEPRGLWGLARRFVSDDLIPASFRPPRQPVMS
                     ARIASENAEAS"
     gene            4806..5339
                     /locus_tag="VKU03_13640"
     CDS             4806..5339
                     /locus_tag="VKU03_13640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2,4'-dihydroxyacetophenone dioxygenase family
                     protein"
                     /protein_id="HLJ72357.1"
                     /translation="MPEAATTEFWKGLKPLATVFQPDALPEIFTANAATDDERYYVPF
                     TETVSSRPLWISPAQNKWCDILMAKRAGLVNRHYHPHQVFAYTISGKWGYLEHDWTAS
                     AGDFVYETPGEGHTLVAYEHKDPMKAIFIVEGPLIWLDEAGEPDGYFDVHKYIAMCKA
                     HYEKVGLGAGLIDKLMR"
     gene            complement(5445..7157)
                     /locus_tag="VKU03_13645"
     CDS             complement(5445..7157)
                     /locus_tag="VKU03_13645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009209241.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-requiring protein"
                     /protein_id="HLJ72358.1"
                     /translation="MPSASAALLDALTEAGVSCIFANFGSDHPALIEAIAEARARRRA
                     IPKIVTSPHEFVAMSAAHGYAQLTGQAQAVFVHVECGTQTLGGAVHNAARARVPLFVF
                     AGLSPFTQEGELRGARNEFIHWLQDVYDQRGIVREYMKYNNEFRTGRNIKTIVRRAMQ
                     FAHSDPPGPVYLTGAREVMEEQVPHVSGEGQYWPTVEPAPLAESAVAELAGALAKAKR
                     PLVVTSYAGRSAEAVRALVRLCERLGIGVLESVPSRMNYPHREAMYQGNHWNHPFQNP
                     ALAEADVVLVIDSDVPWIPTINRPRADATIFHIDIDPLKASTPLWRINARRTFRVDAA
                     SALLQMNAYLDRDGFDEAAAEARRRFWREKHERRAADIAREEVAKDETITPEYLTTCL
                     RRQIGDDAIVLNEGITNYHVICDHMARNEPHSIFASGGGSLGWNGGAAIGMKLAAPER
                     TIVALTGDGSYMFSVPSTVHWMARRYQTPFLQVVYNNRGWKAPKFSALAVHPDGYASK
                     AADLDLSFEPSPDYAGLAAAAGGAFGLTLHKASEVELGVKEALRVIREENRAAVLDVW
                     LPPS"
     gene            complement(7183..7758)
                     /locus_tag="VKU03_13650"
     CDS             complement(7183..7758)
                     /locus_tag="VKU03_13650"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HLJ72359.1"
                     /translation="MKESETSKRPKGERGGGDHVVSPREAPQRAAKRGAARSGRAVSH
                     GEPKATINLGPLENYIAFHLRLAQEIAVRAFVRRSGKADFSPGWFAVLMTIRLNPGIS
                     QSALARAIGRDKSTVSPLIRQLHAAALVVRRPSIADRRSVTLALTKRGESELETLLRH
                     VEAHDRRLDEIVGEDKPKLIALLRKIADALA"
     gene            complement(7755..8513)
                     /locus_tag="VKU03_13655"
     CDS             complement(7755..8513)
                     /locus_tag="VKU03_13655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015342630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72360.1"
                     /translation="MGRLQDKVCLVTGGAGSLGAATAKLFLAEGAKVMLADLEEAALR
                     RAKAALAGGDVETIICDVTRAASVESAVEAAARRWGALDVLFSNAGNFGVVAPIAAYP
                     EEVFDAVYAVHVKGAFLLCRSAAPKMRDGGSIVIVSSVAGLRGDPGVHAYIAAKHAQI
                     GLMRCLAKELAPRRIRVNTLHPGPVDNSFQRNVEGGLTKTIGRDGGEFFNAMIPLGRH
                     ARPEEIAQSALYLASDQSSFTTGALLMVDGGMSA"
     gene            complement(9025..10131)
                     /locus_tag="VKU03_13660"
     CDS             complement(9025..10131)
                     /locus_tag="VKU03_13660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ72361.1"
                     /translation="MSLSIRALLLASVGALIFGTAARADDYVDMATKAAKAATARADK
                     WDGPTAGPKAAKGKKIVFVAGDLKNGGILGASEGVKEAAAAIGWTVTVIDGQGSVSGR
                     TAAMDQALTLKPDGIVVGGFDTNEQKVAFESAAKSGAAIVGWHSGTRPGPEPEAGIFA
                     NVTTLPQDVSNTAAYQAIAESDGKAGVVIFTDSQFEIAVFKAKAMEAAIKKCAGCTVL
                     EYVDSPIAESSQRMPQLTTTLLQKYGDKWTHSLAINDLYFDFMGPSLAAAGKKGDGAP
                     RAISAGDGSESAFQRIRAKQYQDGTVPEPLNMQGWQLVDELNRAFNKAPWSGYVSGIH
                     LVTAENVAYDGGDKNIFDPDNHYRDEYKKIWGVK"
     gene            complement(10128..11222)
                     /locus_tag="VKU03_13665"
     CDS             complement(10128..11222)
                     /locus_tag="VKU03_13665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015917925.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72362.1"
                     /translation="MASSVKSTALEPTRVELAGLSLWARIRRLAPVYGLVILTVLLAL
                     MFSILLPDTFPTMLNLRSIIADKAIIAILSLGVMIPMAAGKIDLTVGYGIVLWHILAI
                     SLQTKFGFPWPLAVLTVLLLGSGVGLINGVLVEVAQIDAFIATLGTGTVLYAIAMWHS
                     QGRQIVGIAPDAFYAINGANLFNLPITGFYVLALALALWIALEYLPIGRYFYAIGANP
                     KAAALNGVPVRRYTIYAFVASGLLTAFAGVILAAKLRIGQASVGLEYLLPALVGAFLG
                     STTIKPGRVNVWGTIVGVTILAIGISGIQQFGAMFFVEPLFNGATLLIAIGIAGYAQR
                     RRAIGGGRTATKAQDSSSGPAASSPRGGQE"
BASE COUNT         1902 a         3701 c         3607 g         2150 t
ORIGIN      
        1 gattcggcga accgctctaa gatagtttgg gctcaaacga ttaggcatgg cgcggccgtc
       61 gcccgccaat gctgcgtttg ggagggagaa acaatgcgct catctcgaaa aatcgctcgt
      121 cgtacggtcc tgaagggaat gctcgccagc ggccttgccg gcgtggccgg caagaacgtc
      181 gcctttgccg ccccgcgcgc gatcaagatc ggcttggtga tgccgcagac cggcattctc
      241 gccgcttttt gcgagcacat gcctttcgtg ctcgctcagg tgaaaaaggt caccggcgac
      301 aagctgacga tcaacggcgc cgtccatccg ttcgagatcg tcatcaagga cagccaatcc
      361 gatcccaacc gcgcctcgga agtcgcccag gagctgatcc tcaacgacaa ggtcgacatc
      421 gtctcgacct tcgctacgcc cgagacggtc aacccggttt ccgatcagtg cgagctcaac
      481 ggcgtgccct gcgtcagcaa cgatgcgccg ctggagccct atttcttcgg ccgccacggc
      541 gatccgaaga agggctggga ctggacctat catttcttct tcagcggcga cgaactggtg
      601 aaggcgatga ttccctattg gaaccgtctg ccgaccaaca aagtcatcgg cgccctttgg
      661 ccaaatgacg gcgacggcat cgcccagtcc aaaggctttc cgccgctgtt cgagaaggcc
      721 ggctacaaga ccgtcgatcc gggccgcttc gacatgcccg cgtcgagcta caacgcgcag
      781 atcgccgcgt tcaaatcggc cggctgcgaa atcatcaccg gcgtcgtgcc gccgccggaa
      841 atgacgacgt tctggaacgg ctgcgcgcag cagggcttta agccgaaatg ctgctatatg
      901 ggcaaagcgc tcgaatttcc cagcgccatc gcgcccctcg gcaagcgcgt cgaaggcctc
      961 accaccgaag tctggtgggc cgcgacctcg ccctactctt caagctggac cggccagacg
     1021 tcgcagcaat tggccgacgc ctatgaagcg gccagcggcc ggcaatggtc gcagccgctc
     1081 ggcttccgcc attccctgtt cgaagtgatt ttcgatacgc tgaaacgcac gcatgatctc
     1141 gacaaggcgg cctcgatccg cgacgcgctg aaaacgacca agctcaacac gatcgtcggg
     1201 ccggtcgact tcgcgacggg accgttgccg aattgcagcc tgacgccgct cgtcgtcggc
     1261 caatggacca agggcaagaa atatcccttt gacatcacga tcgtcgacaa tacgctctat
     1321 cccaatgttc cggtcggcgg cgctccctcg gtgattcact acgcctgatc tcaggagcgt
     1381 tcgcgaaaaa gggaggctgg atgctcgagc tcgtcgagct cagtcggcgc tttggcgcgc
     1441 tcaaggtcat tgacgggctc agcctctcag tcgccgaggg cgacatcctc ggcgtcctcg
     1501 gcccgaacgg cgccggcaag acgacgctgt tcaatctcgt gctcggcgtg cttgcgccgg
     1561 attcgggacg catcgccttt caaggacgcg acattacgcg tatgcggccc tgggaccgct
     1621 gccgcgccgg cataggccgg acgtaccagg tcccacggcc cttcgctcat atgtcggtct
     1681 atgagaacgt gctcgtcgcg gcggttcatg gcggccggtc gagcgtccgc gccagccgcg
     1741 aatgggcgcg ccaagtggtc gacttgaccg ggctcggccg gcggctcgac catcgcgccg
     1801 gcgacctttc cctgctcgac ctgaaacgcc tggagctcgc caaggcgctc gcctgcaagc
     1861 cgaagctgct gttgctcgac gagatcgccg gcggtctcac tgatctcgaa tgcgaccaat
     1921 tgctcgacat cgtccgcaac gcccgccaga gcggggcgac gatcgtttgg atcgagcatg
     1981 tgatccacgc tttgcgccgg atcgccagcc gcctcgccgt gcttcatggc gggcggatca
     2041 tctcggccgg cgcgccggac gaggttctcg ccgatccgcg cgtgcgggaa gtctatctcg
     2101 gcgtcgaggc cgaagccctg tgagcgacgc tcttctgaac atcgagaatc tctcggtttt
     2161 ctatggcgag tttcgcgcgc tgttcggcgt cgatatcgcg gtcaaagccg gcgagatcgt
     2221 ctcgatgatc ggcgccaacg gcgcagggaa gtcatccttg ctgaaagcga ttgtcggcca
     2281 ggtcggccgc atcgaaggcc gcatcgcctt cgacggcgac gacctcgccc gcctgtcgac
     2341 gccggaaatc gtcgcgcgcg gcgtcgccct cgttccggag ggacgcaggc tgtttccatc
     2401 cctgacggtt gaggagaatc tcacgatcgg ccaggagatc ggccgcggcg gcgatctcgg
     2461 cttcgacgag atctaccgct atttccccat tctatgggaa aaacgacgcc agaagacgcg
     2521 tgaattgtcc ggcgggcaac agcagatggc ggcgctcgcc cgcgctctct tggccaaccc
     2581 gcgcctgctt ctttgcgacg agatcagtct cggcctggcg cccgtcgcga tcgccgcgct
     2641 ctatcaggtc atcccgacga tccgcgaccg cggcgtcgcc attctcctcg tcgagcagga
     2701 catcacccgc gcgctggcgg tggccgatcg cttctattgt ctgctcggcg gcagggtgaa
     2761 cctttccggc ctgccgcgcg agacgacgcg ggaaaccgtc gcgcatcact atttcggagg
     2821 ctgagcaggc gaatggcggc gatttggatc aatcagatcg tgcaaggcgt cttgctcggc
     2881 gggctttacg cgatgttcgc gatgggcctt tccctgtcga tcggcgtcat gcaattcgtc
     2941 aatatcgcgc atggcgacct gatcgtcctc gtctcgtttc tcctcctgac gttgaccagc
     3001 gggctcggcc tgccgccgct cctcgccgcg gcgataacct tgccgatcgc tttcgttggc
     3061 ggcttcctgc tgcagcgcct gcttctgcag cgggtgctgg gcaagggcgt gctgccgatc
     3121 atcctggtga cgttcggcct gtcgatcatc atccagaacg ggctgctcag cgcctatggc
     3181 gccgatacgc ggaaagtcgc cggcggcgcg atcgagacgg cgacgattcc gatcggcggc
     3241 ggggtgaata tcggcgttct gcctttcatc atcttcctgt ccgccatcgt cttgatcttc
     3301 gcgctcgatc ggctcctgta ccatacccgg ctcggcgcgc ggatccgggc ggtctccgac
     3361 gacgtcgcca ccgccaatct catcggcctc aaatcggcgc gcatctatgc cgttgcgatg
     3421 ggcgtcgtcg gcgcgaccat cgcgatctcg gcggcgttca tgtcggtttg gaccaatttc
     3481 gacccgagca gcgggccgag ccgcctgctc gtcgccttcg aggcggtggt gctcggcggc
     3541 ctcggcagcc tttggggcac gctggtcggc ggcgtcgcgc tcggcgtcgc ccaggcgatc
     3601 ggcgcgcaat tcgacgccgc ctggcagatt ctcgccggcc atatcctctt cctcgtcatt
     3661 ttcgtgatca ggccgcaagg cctgttcccg cgcaattgag gcttcgacca aggtgaacgc
     3721 ttccggcaca tggctcattg cgatcgggcg tctcgccttg atcctgggcg ccggcgtgct
     3781 ggcggcggcg ccgtttctgt tcgcgccggc gcaactgcaa atcctcaccc agttcctctc
     3841 gctgctcgtc atcgcgatca tgtggaacct tttggccggc tacgctgaca tcgtctcgat
     3901 cggccagcac gcctttgtcg gcatcggcgc ctatgcgttc ttcgcctttg cggctttggc
     3961 gggagtcaac ccttatctct cgctgctgct tggcggcgtg accgctttcg tcttcgccgt
     4021 gccgatcatg ctggtgattt ttcgcctgcg cgccgcctat ctcgccgtcg gcagctgggt
     4081 cgtcgccgag accttgatgc ttggggccgg aaagctgtcc gctttcggcg gcggctcggg
     4141 cgtcagcgtt ccgatctcgg tcgtcaaagc cttcggcgcg caagcggccg cgcgctacgc
     4201 gacgatctat tggctcgcct tcgctttggc cgcgctcgcg ctcgtctcga cctatctgct
     4261 gatgcgctca aaagtcggca tcggcctcac cgcgatgcgc gacaatgagg agggggccgg
     4321 cagcgtcggc gtcaatctca tcgcgctgcg ggttctctgc tttctatgga ccgcgccctt
     4381 ccttggcata gccggcggcg tcatcacgct gcaaaagctg cgcgtcgcgc cgcccgcctc
     4441 gttcagcatc accgattgga cggtctatgt gatcttcatc gtcgtgatcg gcggcattgg
     4501 cagtttcgaa ggcccgatca tcggcgcgat cgtgttcttc ctgatccgcg aatatctggc
     4561 cgagatcggc ctttggcatt tcatcctgct cggcgttctt tcgatcgcgg taattctggt
     4621 cgagccgcgc ggactttggg gcctggctcg gcgcttcgtc tcggacgatt tgatcccggc
     4681 ctcgttccgc ccgccgcgtc aacccgtcat gtcggctcgc atcgccagcg agaacgccga
     4741 ggcgtcatag cggatcgccg agccgatcgc gttgttaaga gcccaggcac gaagaggaga
     4801 ctgcaatgcc cgaagcggcg acaacggaat tctggaaagg cctcaagccc ctcgcgaccg
     4861 tttttcaacc ggatgcgctg cccgaaatct tcaccgccaa cgcggcgacc gacgacgagc
     4921 gctattatgt gccgttcacc gagacggtct cgtcgcgccc gctctggatt tctcccgcgc
     4981 agaacaaatg gtgcgacatc ctcatggcca aacgcgccgg gctggtgaac cgccattatc
     5041 atccccatca ggtcttcgcc tacaccatct caggcaaatg gggctatctg gagcatgatt
     5101 ggaccgcgtc cgctggcgac ttcgtctacg agacgcccgg cgagggtcat acgctggttg
     5161 cgtacgaaca caaagatccg atgaaggcga ttttcatcgt cgagggcccc ctcatctggc
     5221 tcgacgaagc gggcgagccg gacggctatt tcgacgtgca caaatatatc gcgatgtgca
     5281 aggcgcatta cgaaaaggtc ggtctcggcg caggcttgat cgacaaactg atgcgatagc
     5341 tccgtcattg cgggcgaaac gaagcaatcc atttcgtagg gcgctgcgct cgatgaatgg
     5401 attgcttcgt cgcttcgccc ctcgcaatgc cggcgacgcg cgcctcagct tggcggcagc
     5461 cagacgtcga gcacggccgc acgattttct tcgcggatga cgcgtaaagc ctctttgacg
     5521 ccgagctcga cctcgcttgc cttgtgcaaa gtcaacccga acgcgccgcc cgccgccgcg
     5581 gcgagtccgg catagtcggg cgagggctcg aagctgagat ctaagtccgc ggctttgctc
     5641 gcatagccgt cgggatgaac ggcgagcgcc gagaatttcg gcgccttcca gccgcgattg
     5701 ttatagacga cctgcaggaa cggcgtctgg tagcgacgcg ccatccagtg caccgtcgag
     5761 ggcacggaga acatatagga gccgtcgccg gtcagggcga cgatcgtccg ctccggcgct
     5821 gcgagcttca tgccgatcgc cgcgccgcca ttccagccga gcgagccgcc gccgctggcg
     5881 aagattgaat ggggctcgtt ccgcgccatg tgatcgcaga tcacatggta gttggtgatg
     5941 ccctcattga ggacgatcgc atcgtctccg atctgccggc gtaggcaagt ggtcagatat
     6001 tcaggcgtga tcgtctcgtc cttggccacc tcttccctgg cgatatcggc ggctcgccgc
     6061 tcgtgctttt cgcgccagaa gcggcggcgc gcttcggcgg cggcctcgtc gaatccgtcg
     6121 cgatcgagat aggcgttcat ctggagaagc gcgctcgccg cgtcgacgcg gaatgtccgc
     6181 cgcgcattga tccgccagag cggcgtcgaa gctttcagcg gatcgatgtc gatgtggaaa
     6241 atcgtcgcgt cggcgcgtgg ccgattgatc gtcggaatcc acggcacgtc gctatcgatg
     6301 acaagaacga catcggcttc ggcgagcgcc ggattttgga acggatggtt ccagtgattg
     6361 ccctgataca tcgcctcgcg atgcggatag ttcatccgcg agggaaccga ttccaagacg
     6421 ccaatcccca gcctttcgca aagccgaaca agagcgcgca cggcctcggc gctgcgcccg
     6481 gcataggacg tgacgaccag cggcctcttc gccttggcca gggcgccggc cagctcggcg
     6541 acggcgcttt cggccagcgg cgccggttcg accgtcggcc aatattggcc ctcgccggaa
     6601 acgtgcggga cttgctcctc catcacttcg cgcgcgcctg tcaggtagac cggtcccggc
     6661 ggatcgctgt gcgcgaattg catggcgcgg cggacgatcg tcttgatgtt ccggccggtg
     6721 cggaattcgt tgttgtattt catatattcg cgcacgatgc cgcgctggtc gtagacgtcc
     6781 tggagccagt ggatgaactc gttgcgggcg ccgcgcagct cgccctcctg ggtgaacggc
     6841 gaaagcccgg cgaagacgaa gagcggcacg cgcgcccgcg ccgcgttgtg cacggcgccg
     6901 cccaaggtct gcgtgccgca ttcgacatgg acgaacaccg cctgcgcctg gccggtcagt
     6961 tgcgcatagc catgcgcagc gctcatcgcg acgaattcgt gcggcgatgt gacgatcttc
     7021 ggaatcgcgc gtctcctggc ccgcgcctcg gcgatcgctt cgatcaaggc gggatggtcg
     7081 ctgccgaaat tggcgaaaat gcaggaaacg cctgcttccg tgagagcgtc gagaagcgcg
     7141 gcgctggcgc tgggcatgga gcgctccttg ttctttcgaa attcaagcca gcgcgtcggc
     7201 gatcttgcgc aggagcgcga tcagcttggg cttgtcttcg ccgacgatct cgtcgaggcg
     7261 acggtcgtga gcttcgacat ggcgcagcaa ggtttcgagt tcgctttcgc cgcgtttcgt
     7321 caaagcaagc gtgacgctgc gccggtcggc aatggaggga cgacgcacga ccagcgccgc
     7381 ggcgtgcaac tgacggatga gcggcgagac ggtcgattta tcgcggccga tcgcccgcgc
     7441 cagcgcgctc tgcgaaatcc ccggattgag gcggatggtc atcagcacgg cgaaccatcc
     7501 cgggctgaaa tcggctttgc cggatcgcct gacgaaggcg cgcaccgcga tttcctgggc
     7561 aagacgcaag tgaaaagcga tgtaattttc gagcggccct aaattgattg tcgccttcgg
     7621 ctcgccgtgc gaaacggcgc ggcccgaacg cgctgcgccg cgctttgcgg cgcgctgcgg
     7681 cgcctcgcgc ggcgaaacga catggtctcc gccgccacgc tcgcctttcg gacgtttcga
     7741 cgtttcgctc tctttcatgc gctcattccg ccatcgacca tcaagagcgc tcccgtcgta
     7801 aagctgcttt ggtcggaagc aaggtagagc gccgattgcg cgatttcctc gggccgggcg
     7861 tgccgcccga gcgggatcat cgcgttgaag aattcgccgc cgtcgcggcc gatcgtcttg
     7921 gtcaagccgc cctcgacgtt ccgctgaaag gaattgtcga ccggcccggg atggagcgtg
     7981 ttgacgcgaa tgcggcgcgg cgccaactcc ttggcgaggc accgcattag cccgatctgc
     8041 gcatgcttgg cggcgatgta ggcgtggacg ccgggatcgc cgcgcaggcc ggcgacgctc
     8101 gacacgatga cgatgctgcc gccgtcgcgc attttcggcg cggcgcttcg acaaagaaga
     8161 aacgcgcctt tgacatggac ggcgtaaacc gcgtcgaaga cctcctccgg ataagccgcg
     8221 atcggcgcca caacgccgaa attgccggca ttgctgaaca gcacgtcgag cgcgccccag
     8281 cgccgcgcgg ccgcctcgac cgcgctctcg acgctcgccg cccgcgtcac gtcgcagatg
     8341 atggtttcaa catcgccgcc ggccaaagcc gccttggccc gccgcagggc ggcttcctca
     8401 aggtcggcaa gcatcacctt ggcgccttcg gcgaggaaaa gcttggccgt ggccgcgccg
     8461 aggctgccgg cgccgccggt caccaggcag accttgtctt gcagccggcc catgcccctc
     8521 tgtgatctcc tcctttggcc gtcgcgcctt ggcgatggcg ggtttcctat cgatagcagc
     8581 ctcggcgggt ttggcaactc gtttgaggcc aaactttctc cggccgctgc gccggccggc
     8641 gcagcggccg gggcgaggag cgtccgatcg gggggcgtca tggaggttgg gggaaaaggc
     8701 cgcactcgcc ggcgtaattc ccttctcccc ctgagcgcga cgttgcgcgg ggagaaggcg
     8761 gcgcgaagcg ccggatgagg cctgcgcagg attcgaaagg ttaggcgcaa gttagcagcg
     8821 ccccgtgccg gtttcgccgc cagcctcccg gcgctaaccc acaccggctt cgcaagtacg
     8881 gcgaggctgc aacgacgcac gaaatcgacc tcgcgccgga ttgccgcgtc tcgacgtata
     8941 ggtcgatgga ctatcggcgc cgcggcgcca cgccggccgt ctcctggagc gagggggcgt
     9001 cacgccacgc ccccgcccat gggcttactt cacgccccag attttcttgt attcgtcgcg
     9061 gtagtgattg tcggggtcga aaatattctt gtcgccgccg tcataggcga cgttctcggc
     9121 cgtcaccaga tggatgccgg agacgtagcc cgaccacggc gctttgttga aagcccggtt
     9181 cagctcgtcg accaattgcc agccctgcat gttcagcggc tcggggaccg tgccgtcctg
     9241 gtactgcttg gcgcgaatgc gctggaacgc cgattccgag ccgtcgccgg ccgagatggc
     9301 gcgcggcgcg ccgtcgcctt tcttgcccgc cgccgccagc gaaggtccca tgaaatcgaa
     9361 atagagatcg ttgatcgcca gcgaatgcgt ccatttgtct ccatacttct gcagcagcgt
     9421 ggtggtcagt tgcggcatgc gctgcgagga ttcggcgatc ggactgtcga catattcgag
     9481 cacagtgcag ccggcgcatt tcttgatcgc cgcctccatc gccttcgcct tgaacacggc
     9541 tatctcgaac tgcgaatcgg tgaagatgac gacgcccgct ttgccgtccg attcggcgat
     9601 cgcctgatag gccgcggtgt tcgagacgtc ctgcggcaag gtggtgacat tggcgaaaat
     9661 gcctgcctcc ggctccgggc cgggcctcgt gcccgagtgc cagccgacaa tcgccgcgcc
     9721 ggatttagcg gccgactcaa aggcgacttt ttgctcgttg gtgtcgaagc cgccgacgac
     9781 gatgccgtcc ggcttcaggg tgagcgcctg gtccatcgcc gcggtgcggc ccgaaaccga
     9841 gccctggccg tcaatcacgg tgaccgtcca accgatcgcg gcggccgcct ccttgacgcc
     9901 ttccgaagcg ccgagaatgc cgccgttctt caaatcgccg gcgacgaaga cgatcttttt
     9961 gccctttgcc gctttcggac cggcggtcgg cccgtcccat ttgtcggcgc gcgccgtcgc
    10021 cgccttggcg gctttggtcg ccatgtcgac ataatcgtcg gcgcgcgccg cggtcccgaa
    10081 tatcaaggcg ccgacgctcg ccaagagcag cgctcggatg gataggctca ttcttgtcct
    10141 cccctgggtg aagaagcggc cgggccgctg ctggaatcct gcgcctttgt cgccgtgcgg
    10201 cctccgccga tcgcgcgcct tctttgcgcg tagccggcga tgccgatggc gatgagcaag
    10261 gtcgcgccgt tgaacagcgg ttcgacaaag aacatcgcgc cgaattgttg gatgccggaa
    10321 atgccgatgg cgaggatggt cacgccgacg atcgtgcccc agacattgac ccggccgggc
    10381 ttgatcgtgg tcgagccgag aaacgcgccg accagagccg gcagcagata ttcgaggccg
    10441 acgctggcct ggccgatgcg caatttcgcg gcgaggatca cgccggcgaa agcggtcagc
    10501 aggcccgagg cgacgaaggc gtagatcgta tagcggcgaa ccggcacgcc gttgagggcg
    10561 gcggccttcg gattggcgcc gatcgcgtag aaatagcggc cgatcggcag atattcgagc
    10621 gcgatccaga gcgcgagcgc gagggcgagc acgtagaagc cggtgatcgg caggttgaag
    10681 agattggcgc cgttgatcgc gtagaaggcg tccggcgcaa tgccgacgat ctgccgtccc
    10741 tggctgtgcc acatggcgat ggcgtagaga acggttccgg tgccgagcgt cgcgatgaaa
    10801 gcgtcgatct gcgcgacctc gaccagcacg ccgttgatca ggccgacgcc tgagccgagc
    10861 agcagcacgg tcaagacggc aagcggccat ggaaagccga acttcgtctg gaggctgatc
    10921 gcgagaatgt gccagagcac gatgccatag ccgaccgtca ggtcgatctt gccggcggcc
    10981 attgggatca tcacgccgag cgacaaaatc gcgatgatcg ctttatcggc gatgatcgag
    11041 cgcaggttca acatggtcgg aaacgtgtcc ggcagcagga tgctgaacat gagcgcgagc
    11101 aggaccgtca ggatgaccag gccgtaaacc ggcgcgagcc gcctgatccg cgcccaaagg
    11161 ctcaagcctg caagctcgac tctcgtcggc tccagcgcag tcgatttgac ggaagaggcc
    11221 atgtttcctc tcctcaggcg gccgtcggcg cggcgacgga ggccgcgtga atcaagcgct
    11281 cgatcgtcaa atcggcgccg gcgatctcct cgacgatccg cccgcgcgaa aagaccaaag
    11341 cgcgatggca gagcctcgcg
//
LOCUS       DATGJW010000456        11203 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_606364, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000456 DATGJW010000000
VERSION     DATGJW010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11203)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11203)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11203
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606364"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(103..1845)
                     /gene="aroB"
                     /locus_tag="VKU03_13670"
     CDS             complement(103..1845)
                     /gene="aroB"
                     /locus_tag="VKU03_13670"
                     /EC_number="4.2.3.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383366.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003856 - 3-dehydroquinate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-dehydroquinate synthase"
                     /protein_id="HLJ72363.1"
                     /translation="MTQLAPLDRDRLVAALGGRSIVLVGMMGAGKTSVGRRLAAKLGL
                     PFVDADAEIEAGAQMTVPEIFARFGEAYFRDGERKVLARLMADGQKVLATGGGAFMND
                     STRQRIAESGVSVWLKPDFDVLMRRVRKRANRPLLQTANPEETVRKLLAERSPVYGLA
                     DLTIESRDGPHESVVDAIVAALTRRVAQRPREEDKARLAIPTAPEAASSRERREVEVA
                     LGSRAYKILIGEGLIGEAGAHIAHLAPGAHCAVVTDETVAGLHLDALTKSLAAAGLRA
                     TNIVVPPGESSKSYRQFERVCDAIIAAHIERRDAVIAFGGGVIGDLAGFCAAAVRRGV
                     RLVQIPTTLLAQVDSSVGGKTAINSVHGKNLIGAFHQPSLVLADTGVLDTLNAREFRA
                     GYAETIKYGLINDRPFFDWLEGAWRDVFAGGAARLNAIATSCAAKAAVVAADETEQGE
                     RALLNLGHTFGHAFERLVQYDGERLIHGEAVAIGMGCAFRFSRKLGLCSGQDTVRIEN
                     HLRAVGLPTEIGDIPGLEPTPDAILSAMRQDKKVERGRLTFILARGIGESFIAKDVSE
                     ADVHAFLSEDIPRA"
     gene            2362..4431
                     /locus_tag="VKU03_13675"
     CDS             2362..4431
                     /locus_tag="VKU03_13675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor G"
                     /protein_id="HLJ72364.1"
                     /translation="MVGLNGSSERRIAGPRLIAVVGPFQGGKTTLLEAILARGGASAR
                     QGSVRDGTSLGDSSPEARAHAMSVEPNAAHVDYMGDRMTFIDCPGSVEFIHDMRNVLP
                     VCDAAVVVCEADARKAPSLQVVLRELEELGVPRILFLNKIDLAGASVRETLEMLQPTS
                     RTPLLLRQIPIWENGVASGFIDLALERAFVYREHAPSTVIEMPDGHVPDEKEARYSML
                     ERLADYDDALMEELISEIEPPRDRVFEDLAKELRGGHVVPLLMGSADRGNGVTRLLKA
                     LRHEAPGVAQTRERLGVDAQGPALAHVARTIHTAHGGKLSLARVLRGAFPDGATVTSS
                     GGGEDRIAGLSRLIGASSAKIPRAEEGDTVAFARLEHIGTGETFTDAKKAPKRIVEIR
                     APAPTQVIALHVKDRKDDVRLAAGLAKLAEEDPALVYRHDQEAGELKLYGQGEMHLRV
                     TIERLSARFGVTVESSKPSIAYRETIRDAVSARGRHKKQSGGHGQFGDVAIEVRPRSR
                     GEGFAFEERVHGGSVPRQYFSSVEAGSRDAMARGPLGFPVVDVAVTLTDGSYHTVDSS
                     DMAFRAAAKIALGEALPKARPVLLEPILAVSIFVPSDAMARASALVSARRGQILGFDP
                     RPGWDGWDVLRAQIPEAEIGDLIVELRSATAGVGAFETKFDHLAEMSGRPAEMVIAAR
                     QKAAAQG"
     gene            4549..5571
                     /locus_tag="VKU03_13680"
     CDS             4549..5571
                     /locus_tag="VKU03_13680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384305.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I NDUFA9 subunit family protein"
                     /protein_id="HLJ72365.1"
                     /translation="MADFPFDSQRPYPTNRLITVFGGSGFIGRHVVRALVKRGWRVRV
                     AVRRPDLGFFLQPIGTVGQIAAVQANLRYPDSVAAALTGAEAAINVVGISAQRGAQTF
                     DAVHVEGARTIAKATKARAIPLIHVSGIGADASSASPYIASKGLAEQAIRAAAPEAVI
                     LRPSVVFGPEDQFFNRFGLLSRYSLALPLFGGGATKMQPVYVGDVAQAAANAIDGQAK
                     SEATYELGGPDQMTLRQVVEFVCRVTERRRLLFNVPFGVASMMAGVTEFASKISFGAF
                     PAALTTTRDEIALLKNDNVVSAEATAAGLTLAGLGVTPQSAEAIAPAYLYRYRKTGQF
                     ESSRFA"
     gene            complement(5743..6408)
                     /locus_tag="VKU03_13685"
     CDS             complement(5743..6408)
                     /locus_tag="VKU03_13685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018182341.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfate transporter family protein"
                     /protein_id="HLJ72366.1"
                     /translation="MIEDALAALAQIFTRPFRATLLKSLALTLGLLVILWFLLDKLVL
                     SFVGFGPPWLATTIAILAGLGLFVGLAFLIAPITSLTAGFFLDDIALIVEQEINPAGP
                     LGRPVPFMPAMLFGLRYAGLSLVVNVIALILLLAPGVNIVAFLAANGYLLGREYFELA
                     AMRYRSFEEAQAMRRHFGLQVFIAGLVIACFVAVPVLNLCAPLFATAFMTRLHKRLAG
                     EKG"
     gene            complement(6405..7559)
                     /locus_tag="VKU03_13690"
     CDS             complement(6405..7559)
                     /locus_tag="VKU03_13690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HLJ72367.1"
                     /translation="MSHHATPALSVIAPTYREAANVPVLFERLKKALDGLDWELIVVD
                     DDSPDGTSDVAFALATADRHIRCLRRVNRLGLAGAVIEGILSSSADFVAVIDADLQHD
                     ESILPRMYHALADGADLAIGTRISDEKAERGLSPTRQRLSNVGAELFKRMSGLSVRDP
                     MSGFFMVRRSIVSAIAPRLSPDGFKILADILLCAPVGIKVVETTYVFRKRQAGESKLS
                     AIVGLDFLGLIAHHATAGILPIRFALFAAVGAFGLIVHLGVLKILVNAYGASSFGAEQ
                     LFATLAAMTSNFLINNEVTYRENRYRNWRMLLGLLLFALVCSVGAVANINIASWVYNR
                     EEVWWVAGLAGALVGVVWNYAVSSAFVWRTRRTKAPLAVASPSSTTTLAS"
     gene            complement(7574..9091)
                     /locus_tag="VKU03_13695"
     CDS             complement(7574..9091)
                     /locus_tag="VKU03_13695"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HLJ72368.1"
                     /translation="MRLSRESALALAVILGFAAARIVFALLLDFGIDEAYTLTLARRL
                     NWSYFDHPPLHQWIAHFAALAFGENAAVRLPFIALFAATGWLVFALTRDLFGAVAGLW
                     ATFALNATPFFFASAGGWIVPDGTLAFALAASAYALTKLLFAPAPRVWPLWLLAGLFI
                     GLAGLSKYNAVFFPLGLLLYLVLSPNQRRWFFHPAPYGAALLALMLVAPVVIWNAEND
                     WVSFLFQSARGAPSPHWRPAQVGAMLLSEIALLSPWIFISLAAGLFAAFRNIARGEDD
                     GRRLLLLCLALPPIVLFTVTPLWGARGLPHWPMPGWLFVYPLLGAWVSESASRPQWRR
                     PFVIAATAALALIAALAIAQARTGFLIAFFPRLGDGADPTLESLSWSKVRESPVLRDH
                     GEAFIVATKWTEAGKIAQALGPERPVTVFSDDPRGFAFLADPAAFVGKDAVIIVSQKR
                     LAETIAALTPYFATLGDTQSVTLGRDGRDEIKLALIPARRLLRAYPLPYPRRASP"
     gene            9330..9746
                     /locus_tag="VKU03_13700"
     CDS             9330..9746
                     /locus_tag="VKU03_13700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72369.1"
                     /translation="MLPLIVFAMVSASVPNLDIAAGCKPEAQIEGQIVDLKACMDDER
                     ATKDKLLKEWSSYSAAARNTCATDQASLVNSYVELLACIEMQTWKADPAVMNPSGPPG
                     AAAAGGPVGSPPTPTQLGGGSLASHPLGGRPGGHMP"
     gene            complement(9743..10711)
                     /locus_tag="VKU03_13705"
     CDS             complement(9743..10711)
                     /locus_tag="VKU03_13705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014893049.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ72370.1"
                     /translation="MRLAATPENPVPPGAILSTARGIDGAPLRAARWVPPTAKRGTVA
                     ILHGRAEFIEKYYEVIGELLARGFAVATIDWRGQGGSTRQLRNRRKGHVDDFSLYERD
                     LSGLVDDVLGPSCPQPWFGLAHSMGAAIMLSIARAGRCPFERLVLTAPMIRLAGLSHP
                     WLARGLAETLDTLGLGGLFAPGGGSASPSILPFEGNVLTSDRARYARTAGMIITAPDV
                     ALGWPTIGWINAAFRVMSEFEDPDFAREITTPTLIIAAGADRVVDTRSAERFAAKMRT
                     GRLIVVDGAQHEILMERDRYRDLFWAAFDQFIPGVESERALGRRSA"
     gene            complement(10901..>11203)
                     /locus_tag="VKU03_13710"
     CDS             complement(10901..>11203)
                     /locus_tag="VKU03_13710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384759.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP phosphoribosyltransferase"
                     /protein_id="HLJ72371.1"
                     /translation="RIAAAQEALASRELRARLPGDAKGVLESAAAMFDAQTAFGEEPD
                     AHGRLTFHAPKQNVAALAAWLLAQGAERVSVSALEHVFSARNALYEGLERRIGARK"
BASE COUNT         2163 a         3457 c         3782 g         1801 t
ORIGIN      
        1 gccggagaaa aaggccgacg cgaccaagct cgccaggacg atcagcgccg cgaattcgat
       61 gctcatggcc ccctcgattg ttgttcttcg gtatatagcc gctcaagcgc gcggaatatc
      121 ttcactcaga aaggcgtgga cgtcagcctc gctgacgtcc ttggcgatga aactttcgcc
      181 aatgccgcgc gcaaggatga aagtcagccg gccgcgttcg actttcttgt cctgccgcat
      241 ggcggaaagg atggcgtcgg gcgtcggctc caggccggga atgtcgccga tctcggtcgg
      301 cagaccgacg gcgcgaagat gattttcgat gcggaccgtg tcctggcccg agcaaaggcc
      361 gagtttgcgc gagaaacgaa acgcgcagcc catgccgatc gccaccgcct cgccgtgaat
      421 gagccgctcg ccgtcatatt ggacgaggcg ttcgaaggcg tggccgaatg tatggccgag
      481 attgagcaag gcgcgctcgc cttgctcggt ctcgtcggcg gcgacgacag cggccttggc
      541 cgcgcaactc gtcgcgatcg cgttgaggcg cgccgccccg ccggcgaaga cgtcgcgcca
      601 ggccccctcg agccaatcga agaaagggcg atcattgatg aggccgtatt tgatcgtctc
      661 ggcataaccg gcgcgaaatt cgcgcgcgtt cagcgtgtcg agaacgcccg tgtcggcgag
      721 cacaagcgaa ggctggtgaa aagcgccgat cagattcttg ccatggaccg agttgatcgc
      781 tgtcttgccg ccgaccgagg aatcgacttg cgctaacagg gtcgtcggaa tttgtacgag
      841 gcggacgccg cggcgaacag ccgccgcgca aaatccggcg agatcgccga tcacgccgcc
      901 gccgaaggcg atcaccgcgt cgcgtctctc aatgtgagcg gcgatgatcg catcgcacac
      961 gcgctcgaac tgccggtacg atttcgagct ctcgcccggc gggacgacaa tattcgtcgc
     1021 gcgcaggccg gctgcggcga ggctttttgt cagcgcatcg agatggagcc cggcgacggt
     1081 ctcgtcggtc accacggcgc aatgggcgcc aggggcgagg tgcgcgatat gcgcgccggc
     1141 ctcgccgatc aggccttcgc cgatgaggat cttataggcg cgcgagccga gggcgacctc
     1201 gacttcgcgc cgctcgcggg aagaggccgc ttccggcgcg gtcgggatcg ccagcctggc
     1261 cttgtcctcc tcgcgggggc gttgcgcgac tcgccgggtc aaggccgcaa cgatggcgtc
     1321 gacgacgctc tcatgcggcc catcacgcga ttcgattgtg aggtcggcga ggccatagac
     1381 gggcgagcgc tcggccagaa gcttgcgcac tgtctcctcg ggattggcgg tctgcagcaa
     1441 ggggcgattg gcgcgcttgc gcacccggcg catgagcaca tcgaaatcag gcttcagcca
     1501 gaccgagacg ccgctttcgg cgatgcgctg ccgggtgctg tcgttcatga aggcgccgcc
     1561 gccggtcgcc agcactttct ggccgtcggc catcagccgc gcgaggactt tacgctcgcc
     1621 gtcgcgaaaa taagcctcgc cgaaacgcgc gaagatttcc ggcacggtca tttgcgcgcc
     1681 ggcttcgatc tcggcgtccg cgtcgacgaa aggcaggccc aatttggccg ccaggcgccg
     1741 gccgaccgat gtcttgccgg cgcccatcat gccgacgagc acgatcgacc gcccgcccaa
     1801 ggcggcgacc aggcggtcgc ggtcaagcgg cgccaattgc gtcatcccag cacccaaatc
     1861 cccaaacacc cgcccggcgc gcctctcccc gaagaccgtc aggccgaatt tgcggaggtc
     1921 catacaacgt tatcaggggg attgcgatgg gcgccaaaca cgcgtcgagc gataggcgtg
     1981 cgatcgccaa gcccccggac gcggcgctgt tggcatggat agcccgacac gcctagccgc
     2041 tttttgccgc tgctaaagca aacaaggcga gggcgacggc cgagcgatga tttgtgatgg
     2101 ccacccgacg ctatggagca ctgtatctcg gcgtgaccaa tgatctcggt cgcagggcgt
     2161 gggagcatga gaacgaagtc tacagaggat tttccgcaaa acataacgtc gatcgccgaa
     2221 cggtgctctc ctctcgaagc cagcgccgcc cccaaatccc atcttgcgct cggttgaagc
     2281 ttttgcgacg ctgaggtgac ttgtgcgggc ctcgccgccg gcgggcggtc tggtcctcgc
     2341 caaaaaatcg aggaggacgt catggtcggc ctgaatggct ccagcgaaag acgaattgcg
     2401 gggccgcggc tcatcgccgt cgtcggcccc ttccagggcg gcaagacgac gctgctcgag
     2461 gcgattttgg cgcgcggcgg cgcctcggcg cggcagggtt cggtgcgcga cgggaccagc
     2521 ctcggcgatt ccagccctga ggctcgcgcc catgcgatga gcgtcgagcc gaacgccgcg
     2581 catgtcgact atatgggcga ccggatgacc ttcatcgatt gcccgggctc ggtcgaattc
     2641 attcacgaca tgcgcaacgt cctgccggtc tgcgacgcgg cggtcgtggt ttgcgaagcg
     2701 gacgcacgca aggcgccctc gttgcaggtg gtgctgcgcg aactcgagga gctcggcgtg
     2761 ccgcgcatcc tctttctcaa caagatcgac ctcgccggcg cgagcgtgcg cgagacgctg
     2821 gagatgctgc agccgacgtc acggacgccg ttgctgctgc gccagatccc gatctgggag
     2881 aatggcgtcg cttcgggttt catcgatctc gccttggagc gcgccttcgt ctatcgcgaa
     2941 catgcgcctt caaccgtgat cgaaatgccc gatgggcacg taccggacga gaaggaggcg
     3001 cgttactcga tgctcgagcg cctcgccgat tacgacgacg cgctgatgga ggaattgatt
     3061 tcggagatcg agccgccgcg cgatcgcgtc ttcgaagatc tcgccaagga attgcgcggc
     3121 gggcatgtcg tgccgctgct gatgggctcg gccgatcggg gcaatggcgt cacgcgcttg
     3181 ctgaaagctt tgcgccatga agcgccgggc gtcgcccaga cgcgcgaacg gctcggcgtc
     3241 gacgcgcaag ggccggcgct cgctcatgtc gcgcgtacta ttcataccgc ccatggcggc
     3301 aagctctcgc tcgcgcgcgt gctgcgcggc gcttttccgg acggcgcgac ggtgacgtca
     3361 tcgggcggcg gcgaggaccg catcgccggc ttgtcgcggc tcatcggcgc ttcgagcgcg
     3421 aaaataccgc gcgccgagga aggcgatacg gtcgccttcg caaggctgga gcatatcggg
     3481 acgggcgaga cgttcaccga cgcgaaaaag gcgccgaaac gcatcgtcga aatacgagcg
     3541 cctgcgccga cccaggtcat cgcgctgcat gtgaaggacc gcaaggacga tgttcgtctc
     3601 gccgcagggc tggcgaaatt ggccgaggaa gatcctgcgc tcgtctatcg ccacgaccag
     3661 gaagccggcg aactgaagct ctatggtcag ggcgaaatgc atctgcgcgt gacgatcgag
     3721 cgtctgtcgg cgcgcttcgg cgtaacggtc gaaagttcga aaccgtcgat cgcctatcgt
     3781 gagacgattc gcgacgcggt atcggcgcgc gggcggcata agaagcaatc gggcggccac
     3841 ggccaattcg gcgacgttgc gatcgaagtc aggccgcgtt cgcgcggcga aggcttcgct
     3901 ttcgaagagc gtgttcacgg cggctcggtg ccgcggcaat atttctcgtc ggtggaggcc
     3961 ggctcgcgcg atgcgatggc gcgcgggccg ctcggttttc cggtggtcga tgtcgccgtc
     4021 accttgaccg atggttcgta ccacacggtc gattcctccg atatggcgtt tcgcgcggcc
     4081 gcgaagatcg ccttgggcga agccttgccc aaggcgcggc cggttctgct cgagccgatc
     4141 ctggcggtct ctatcttcgt gccctcggac gcgatggcgc gggcgagcgc tttggtcagc
     4201 gcgcgccggg ggcaaatcct cggctttgac ccgcggccgg gctgggacgg ctgggacgta
     4261 ttgcgcgcgc aaatccccga ggcggagatc ggcgatctga tcgtggaatt gcgctcggcc
     4321 actgctggcg tcggcgcttt cgagacgaaa ttcgaccatc tcgccgaaat gtccggccgg
     4381 cccgccgaaa tggtgatcgc cgcgcgtcag aaagccgcag cccagggata aaaagagccc
     4441 cgcgccaatg ccgcgattgc ggcgttggcg cgactataca gaggcgcgct cttcgcctat
     4501 tcacttgttc ggtgagactt aagcgtcgcc ggcgcggaga cgaccttcat ggctgacttt
     4561 ccctttgatt ctcagcggcc ttacccgacc aatcgcctga tcacggtttt cggcgggtca
     4621 gggttcatcg gccgccatgt ggtgcgcgcc ctcgtcaagc gcggctggcg cgtgcgggtc
     4681 gcggtgcgcc ggcccgatct cgggtttttt ctgcagccga tcggcaccgt tggccagatc
     4741 gccgcggtgc aggccaacct tcgctatccc gactccgtcg ccgccgcgct gaccggagcg
     4801 gaggcggcga tcaatgtcgt cggcatcagc gcccaacgag gcgcccagac tttcgacgct
     4861 gtgcatgtcg agggcgcgcg gacgatcgcg aaagcgacga aagcgcgcgc gatcccgctg
     4921 attcatgttt cggggattgg cgccgacgct tcatccgcct cgccctatat cgcgagcaaa
     4981 gggctcgccg aacaggcgat ccgcgcagcg gcgccggagg ccgtgatcct gcgtccctcg
     5041 gtcgtctttg gacccgagga ccagttcttc aatcggttcg ggttgctctc gcgctattcg
     5101 ctcgctttgc cgctgttcgg cggcggcgcg acaaaaatgc agccggtcta tgtcggcgat
     5161 gtcgcccagg cggcggccaa cgctatcgac ggccaggcca aatccgaggc gacctatgag
     5221 ctcggcggtc ccgatcagat gacgctcagg caagtggtcg aattcgtctg ccgcgtaacg
     5281 gagcggcgcc gcctgctctt caatgtgccc ttcggcgtcg cttcgatgat ggcgggggtg
     5341 acggaatttg cgagcaagat ttccttcggt gctttcccgg cggcgctgac gacgacgcgg
     5401 gacgaaatcg cgctgctcaa aaacgacaat gtcgtctcgg ccgaggcgac ggccgcgggg
     5461 ctgaccttgg cgggattggg cgtgacgccg caaagcgccg aggcgatcgc cccggcttat
     5521 ctctaccggt atcgcaagac ggggcagttc gagagcagcc ggttcgcgta ggggtggcgt
     5581 actttttgat gccgctgcgt accttatcgt ttcagcatcg agaccattgc tggtacaagc
     5641 gacaacatcg ctgcgcgcga ctccccgcat ccatcacgac ttcgtcgtga cacgttcctc
     5701 agcaaagagg gaggaagggc gctgccctaa ccgctcgtgc gatcaaccct tctcgcccgc
     5761 cagccgctta tgcaaccgcg tcatgaaggc cgtcgcaaac agcggcgcgc agaggttcag
     5821 gacggggacc gcgacgaaac aggcgatcac caatcccgcg ataaagacct gcaggccgaa
     5881 gtgccggcgc atcgcttgcg cttcctcgaa agatcgatag cgcatcgccg cgagttcaaa
     5941 atattcgcgc ccgaggagat agccgttggc ggcgaggaag gcgacgatat tgacgccagg
     6001 cgcaagcaaa aggatcagcg cgatcacgtt cacgacgagc gagagaccgg cgtagcgtaa
     6061 gccgaagagc atggccggca tgaaaggtac cggccgaccg agcggtcccg ccggattgat
     6121 ctcttgttcg acgatcagcg cgatatcgtc gagaaaaaag ccggcggtca gcgaggtgat
     6181 cggcgcgatc aggaaggcga ggccgacgaa aagcccaaga ccggcaagaa tggcgatcgt
     6241 cgtcgccagc cagggcggcc cgaagccaac gaaggacaag accagcttgt cgagcaagaa
     6301 ccacaggatg accagcaggc cgagcgtcag cgcaagcgac ttcagcaaag tcgcccgaaa
     6361 ggggcgcgtg aaaatctgcg cgagcgcggc gagcgcgtct tcgatcatga agcgagggtg
     6421 gtggtcgaag atggcgaagc gacggcaagc ggcgccttag tccgtcgtgt gcgccagaca
     6481 aaggcggaac tgaccgcata attccagacg acgccgacca gggcgccggc gagcccggcg
     6541 acccaccaga cttcttctct attatagacc cacgacgcga tgttgatatt ggcgaccgcg
     6601 ccgacggagc aaacgagtgc aaagagcaaa aggccaagca gcatccgcca attgcgataa
     6661 cggttctcgc ggtaagtcac ttcgttattg atcagaaagt tgctggtcat cgccgcgaga
     6721 gtggcgaaga gttgttcggc gccgaaactc gacgcgccat aggcgttgac caatattttc
     6781 agcacgccca gatggacgat caaaccaaaa gccccaaccg cggcgaagag cgcaaaccgg
     6841 atcggcagga tgccggccgt ggcgtgatgg gcgatcaagc ccaggaaatc gagaccgacg
     6901 atggcggata gtttggattc gccggcctga cgcttgcgaa atacgtacgt cgtctcaacg
     6961 acttttattc ccaccggcgc gcagagaaga atatcggcca aaatcttgaa cccgtccggg
     7021 gaaagccgcg gcgcgatggc ggacacaatg gagcggcgga ccataaagaa gccgctcatc
     7081 ggatcgcgca ccgagaggcc ggacatgcgc ttgaagagtt cggcgccgac gttgctgagc
     7141 ctttggcgcg tcggcgaaag gccgcgctcg gctttctcgt ccgaaatgcg cgtgcctatg
     7201 gcgagatcgg cgccgtcggc aagggcgtgg tacatacgcg gcagaatgct ctcgtcgtgt
     7261 tgcaaatcgg cgtcgatcac cgcgacgaaa tcggcgctag aggaaagaat gccttcgatc
     7321 accgcgccgg cgaggccgag gcggttgacc cggcgcaggc agcgtatatg ccggtcggcc
     7381 gtggcgagcg cgaaagcgac atcggacgtg ccgtcgggcg aatcatcatc gacgacgatc
     7441 aattcccagt cgagcccgtc gagagccttc ttcaaacgct caaaaagcac tggaacattg
     7501 gccgcctcgc gataggtcgg cgcaatgacg gaaagcgctg gggtcgcatg atggctcata
     7561 aagcggctcg agactacggc gacgcgcgcc gcggataagg caaaggatag gcacgcaata
     7621 agcgccgcgc gggaataagc gccagtttga tctcatcgcg gccatcgcgg ccgagagtga
     7681 cggattgagt atcgcccagg gttgcaaaat aaggcgttaa cgccgcgatc gtttcggcga
     7741 gccgcttttg cgagacgatg atcacggcgt ccttgccgac gaaagccgcg ggatcggcga
     7801 gaaaggcgaa gccgcgcggg tcgtcggaaa agaccgtcac gggccgctcc ggccccagag
     7861 cttgtgcgat tttgcctgct tcggtccatt ttgtggcgac gatgaaggcc tcgccatggt
     7921 cgcgcagcac aggcgactca cgaactttcg accagctcag gctttcgagc gtggggtccg
     7981 cgccatcgcc gagacgcggg aagaacgcaa tgagaaagcc ggttcgcgcc tgggcgatgg
     8041 cgagggccgc gataagggcg agcgccgccg tggcggcgat gacaaagggg cgccgccatt
     8101 gcgggcgcga tgcgctttcg ctgacccagg cgccaagcag cggataaacg aagagccagc
     8161 ccggcatcgg ccaatgcggc aggccgcggg cgccccacag cggggtcacg gtgaagagta
     8221 cgatcggcgg caaggcgaga caaagaagca agagccgccg cccgtcgtct tcaccccgcg
     8281 cgatgttacg aaaagccgcg aagaggccgg cggcgagaga aatgaaaatc cacggcgaaa
     8341 gaagcgcgat ttcgctcaaa agcatggcgc cgacttgcgc cggccgccaa tgcggcgaag
     8401 gcgcgccgcg cgcgctttga aacagaaacg agacccagtc gttttccgcg ttccaaatca
     8461 cgaccggtgc gacgagcatc agcgcgagca gcgccgcgcc atagggcgcg ggatggaaaa
     8521 accatcgacg ttggttgggc gagagcacga gatacaaaag gaggccgagg ggaaaaaaga
     8581 cagcgttgta tttcgagagg ccggccaagc cgatgaagag tccggcgagg agccaaagcg
     8641 gccagacgcg cggggcgggc gcgaagagta gtttcgtcaa cgcataggcg gatgcagcga
     8701 gcgcgaaggc gagcgtacca tccggcacga tccatccgcc tgccgaagcg aagaagaacg
     8761 gcgtggcatt cagcgcgaag gtcgcccaaa gcccggcgac cgcgccgaag agatcgcggg
     8821 taagcgcgaa gacgagccat cccgtcgccg cgaaaagcgc gatgaagggc aggcggacgg
     8881 cggcattctc gccaaaagcc aaagccgcaa aatgggcgat ccattgatgc agcggcggat
     8941 ggtcgaaata ggaccagttc aggcgccgcg cgagcgtcaa tgtgtaggct tcgtcgatcc
     9001 cgaaatcgag cagcagggcg aagacaatcc tcgccgccgc aaagccgagg atgaccgcca
     9061 gagccagggc gctttcgcgg gagaggcgca tcgcgcccgc cgacgccgcc ggggcggcgt
     9121 cgccagcggc tactttcacg cctggcgaat gcgacaaatt ccaggtccga atcagcccgg
     9181 cggatgaccc gcgaagctcg gatgaaatgg agtccgggcc gcgccctgtc aattgacgag
     9241 gccggcgcgc ctaagggcca gtctaaagaa ccttttatct ggtcggcgcg ttttgagcgg
     9301 tgagaattta tgccacccgg aggttcgata tgctgcctct cattgttttc gccatggttt
     9361 cggcgagcgt gcccaatctc gatattgcgg cgggctgcaa accagaggcc caaatcgagg
     9421 gccagatcgt cgatctcaaa gcctgtatgg acgacgagcg ggcgaccaag gacaagctgc
     9481 tgaaggaatg gtcgagctat tcagcggcgg cccgaaatac ctgcgcgacc gatcaggcca
     9541 gcctggtgaa cagctatgtc gagctcctcg cctgcattga aatgcagacc tggaaggccg
     9601 atccggcggt catgaatccc tccggaccgc caggagcggc cgcggccggc ggcccggttg
     9661 gatcgccgcc gacgccgact caactgggcg gaggctcgct cgcctcccat cccctcggcg
     9721 gcaggccggg cgggcacatg ccttaggccg atcgccggcc aagggcgcgt tcgctttcga
     9781 cgccaggaat gaattggtcg aaggccgccc agaacaggtc gcgatagcga tcacgctcca
     9841 tcaaaatttc gtgctgggcg ccgtcaacga cgatgagccg gccggttctc atcttggcgg
     9901 cgaaacgctc ggccgaacgc gtatcgacga cgcgatcggc gccggcggcg atgatcagcg
     9961 tcggcgtcgt gatctctcgc gcaaaatccg gatcctcaaa ttcgctcatc acgcggaaag
    10021 cggcgttgat ccagccaatg gtcggccagc cgagcgcgac atccggcgcg gtgataatca
    10081 tgccggccgt gcgggcgtag cgggcgcgat ccgaggtcag aacattcccc tcaaaaggta
    10141 ggatcgaggg gctggccgaa ccgccgcccg gcgcgaacag gccgccgagg ccgagcgtat
    10201 ctagcgtttc ggcgagccca cgcgccagcc agggatggct gaggccagcg agacggatca
    10261 tcggcgcggt gagaacgagg cgttcgaacg ggcagcgccc ggcccgcgcg atcgacagca
    10321 tgatcgcggc gcccatcgaa tgagcgagac cgaaccaggg ctgcggacag gaggggccga
    10381 gaacgtcgtc gacgaggccg ctgagatcgc gctcataaag cgagaaatcg tcgacatggc
    10441 ccttgcgccg gttcctcaac tgccgcgtcg agccgccttg gccgcgccag tcgatcgtgg
    10501 cgacggcaaa accgcgcgcc agcaattcgc cgatcacttc gtagtatttt tcaatgaatt
    10561 cggcccggcc atgcaggatc gccacggtgc cgcgtttggc cgtcggcgga acccaccgcg
    10621 ccgcgcggag cggcgcgccg tcgatgccac gcgcggtcga aaggatcgcg ccgggcggga
    10681 cggggttttc gggcgtggcg gcgagtcgca tgcagagggc cttgcggaga ggttgtggca
    10741 tggcgggaag gggagggcaa tgtgactatc gagcgcgccg cgcgggcgat caggaccgcg
    10801 gcggcagcct cgccgcacgt cgagaagaac gcccgtacca tcctgcgtga catcgtcgag
    10861 cgagacctgc gcaggggagg gccggcgaaa cgtggagctt ttatttccgt gctccgatcc
    10921 tgcgctcaag cccttcgtac agcgcgttgc gcgcgctgaa gacatgctcc aaagcgctga
    10981 cgctgacgcg ttcggcgcct tgggcgagga gccacgcggc gagtgcggcg acgttttgtt
    11041 tcggcgcatg aaaggtgagc cggccgtggg cgtcgggctc ttcgccgaaa gcggtttgcg
    11101 cgtcgaacat ggcggcggcg gattcgagga cgcctttggc gtcgccgggg aggcgggcgc
    11161 gtagttcgcg cgaggcgagc gcttcttgcg cggcagcgat gcg
//
LOCUS       DATGJW010000457        21181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_606402, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000457 DATGJW010000000
VERSION     DATGJW010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 21181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 21181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..21181
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_606402"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(225..689)
                     /locus_tag="VKU03_13715"
     CDS             complement(225..689)
                     /locus_tag="VKU03_13715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003568532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ72372.1"
                     /translation="MTYLLDTDIVSAVAPTKSDRPAALARWLDDASSGLYLSAATAAE
                     IQAGIAKAARLGAARKASLLRDWWDSVEHLYGARILPFDLRTAAVAGKIIDIARSAGH
                     EPGFADVVIAATAQTHALTILTRNIRHFEPLGGPVLNPIDQLPPPLSAGKRR"
     gene            complement(686..928)
                     /locus_tag="VKU03_13720"
     CDS             complement(686..928)
                     /locus_tag="VKU03_13720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003567966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0110001 - toxin-antitoxin complex
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system Phd/YefM family
                     antitoxin"
                     /protein_id="HLJ72373.1"
                     /translation="MREIQLKDAKATLSAIVDETLNGEAFAITRHGRKQAVVISWDTW
                     RRLSNVPSFGRLLASSPLEEGDIPARDRSPSRDVDL"
     gene            complement(997..2301)
                     /locus_tag="VKU03_13725"
     CDS             complement(997..2301)
                     /locus_tag="VKU03_13725"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018409681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HLJ72374.1"
                     /translation="MNPHDAILTRFAPLHPKKIDLSLGRIERLLADLGAPEKRLPPVV
                     HVAGTNGKGSTIAFMRAALEAAGKRVHVYTSPHLVRFNERIRLAGKLVDDERLAEAFD
                     RCERVNGGQAITVFEITTAAALLLFSEAPADCLLLEVGLGGRYDATNVIDKPAAAIIT
                     SISIDHVEFLGATVEKIAYEKAGVLKRGAPAIIAEQDARALAVIEREAQRVGAELIVN
                     GRDFSLREENGRLVFEDELGLIDLPLPRLPGRHQHGNAANAVAALRRIEPHISLRAFE
                     RGLTEAEWPARLQRLRSGKLVDLAPARAELWLDGGHNEDGGRVLAAAMADFEEQSPRP
                     LVVICGTLATKDTAGFLRHFKGLAQELLAVPIAGEHAGRSADEVAQIAASVGIEAAAS
                     PGVEAALRFLSARSWPAPPRVLIAGSLYLAGATLEANGTALE"
     gene            complement(2328..3191)
                     /gene="accD"
                     /locus_tag="VKU03_13730"
     CDS             complement(2328..3191)
                     /gene="accD"
                     /locus_tag="VKU03_13730"
                     /EC_number="6.4.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009317 - acetyl-CoA carboxylase complex
                     [Evidence IEA];
                     GO_function: GO:0003989 - acetyl-CoA carboxylase activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA carboxylase, carboxyltransferase
                     subunit beta"
                     /protein_id="HLJ72375.1"
                     /translation="MNWMSDVVPPKIRSFLRRDTPENLWVKCPESGQLVFHKDLEANL
                     FVVPGSGYHMAIGAKARLANLFDGGEYEELPTPEVPADPLKFRDVKRYIDRLKDYRTK
                     TGSADAVILAVGGLEGLTVTVAVQDFAFQGGTLGMAAGEAIIAGMTAAVERRTPFIIF
                     TASGGARMQEGMFSLMQMPRTTVAVQRLRAAKLPYIVVLTNPTTGGVTASYAMLGDIH
                     IAEPGAVIGFAGARVIEQTIRERLPEGFQRAEYLKKHGMVDMVVPRHQLRPVLARLCK
                     LLTSGAAAAAA"
     gene            complement(3211..4047)
                     /gene="trpA"
                     /locus_tag="VKU03_13735"
     CDS             complement(3211..4047)
                     /gene="trpA"
                     /locus_tag="VKU03_13735"
                     /EC_number="4.2.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004834 - tryptophan synthase activity
                     [Evidence IEA];
                     GO_process: GO:0000162 - tryptophan biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan synthase subunit alpha"
                     /protein_id="HLJ72376.1"
                     /translation="MTTRIDARFAALKAEGRAALVTFITAGDPDLETSTALLKALPGA
                     GADVIELGMPFTDPMADGPAVQASGLRALNGGQTMRKTLAMVAEFRKADPATPMVLMG
                     YYNPIYVHGVEAFLHDAKAAGVDGLIVVDLPPEEDEELCLPALKAGLNFIRMATPTTD
                     EKRLPAVLANTSGFVYYVSVTGITGAAAPDAGKVGAAVARLKAHSHLPVAVGFGVKNA
                     ASAAAIAAKADGVAVGSALVEAVRTSLNGGKATAGTVGAVTALVQELAAGVRGAKQKV
                     SA"
     gene            4193..5188
                     /locus_tag="VKU03_13740"
     CDS             4193..5188
                     /locus_tag="VKU03_13740"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72377.1"
                     /translation="MAFQNISLGAGRRGLAGLVGRPGGIRRPAAFFIIICIVAGVLAP
                     AFFSLNNFIALLTNSSFLIVVAAGESLVILVGMIDLGVESVLAASGMFIAWLTVFHGV
                     PAGAAIVLTLAAGAAVGVLVGLLVARGGIPSFIVTLATYWGLRGVALLFNGGNYISPD
                     AVSPPRPFGFAGIAANIGGVPILILIALIIVILAQAVVSLTPLGAWIRASGSNENAAR
                     AVGVRTDAIKILTFAISGFMAALAGIMITAWQDSIYPLTGEGYSLQAIAGVILGGIPF
                     NGGRGTIVGAALGVLVIGVINDVIVLIGLPALYEYIFVAAILVLAGLQARLASAK"
     gene            complement(5286..6032)
                     /locus_tag="VKU03_13745"
     CDS             complement(5286..6032)
                     /locus_tag="VKU03_13745"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72378.1"
                     /translation="MSAETAAAAPPPLLSVRGVVKTFGPVRAVDGVSLDFYPGEVVGI
                     VGDNGAGKSTFLALLTGFHHPDSGRFLYRGREVRVRSPRTARADLKIEMIYQRLELAP
                     DLKVWENLFLGAELRRFGVWLARRLMREQAAGVLKQLNTKILPEAIVGELSGGEQQAV
                     AIGRAILFERQIIIMDEPTAAISVQKVGDVLALIARLRAAGKTVLLVSHRLEDLLQVC
                     DRIVVFRQGKVRTILENKGLSVGDLVHAMF"
     gene            complement(6029..7081)
                     /locus_tag="VKU03_13750"
     CDS             complement(6029..7081)
                     /locus_tag="VKU03_13750"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ72379.1"
                     /translation="MKTLTAVCLAALAAGAAPALAATASSEAPKPVYFIFTGYAYPYF
                     APMAEAVKQAAAHYPDMTIKIVNANNSASQEIADINEAVAAGAKGIILNTIQESVTAT
                     AERAMKGGVPIVTVDRDVSRPDARVAFVGDNDETLGREETQYGVDTLAKAKVPKPWHV
                     AILQGTLGASVSIGRLKGAMDVLQPLIDKKEVDVVLNQSADFASDKAQAMMSAELAKT
                     TNIQLVVCGNDAMALGVIQALKAHNVTPGEKTYVVGADAQPESMDAIKAGTQLDTVTH
                     APFVEAYWAVEAMSAYLKNGEKPPEAKFPHGDIIIPMTLVDKANVGQIAAWGTPEEIP
                     PLPYGQAQAFKVTSGK"
     gene            complement(7250..7891)
                     /locus_tag="VKU03_13755"
     CDS             complement(7250..7891)
                     /locus_tag="VKU03_13755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HLJ72380.1"
                     /translation="MADRRAFILANTRVLPVPHAPEIKLHLADEATALWKKTEEELGR
                     IGLPPPFWAFAWAGGQALARYLLDHRRVVAGKRVLDFAAGSGLVGSAAAMGGAASVEA
                     CEIDEFAVEAIALNAALNGVQVAPRLADLVGTDEGWDVVLAGDVSYQKDMAEAVTDWL
                     ASLAKRRAEVLIGDPGRAYLARDRLEPIAEYRVPATRALEDAEIKRTAVYRFR"
     gene            complement(7901..8380)
                     /locus_tag="VKU03_13760"
     CDS             complement(7901..8380)
                     /locus_tag="VKU03_13760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0262 family protein"
                     /protein_id="HLJ72381.1"
                     /translation="MSAPADRRRLVDVALDDPLARGTPDQEHERAIAIYDLLQENSFG
                     LPDRDDGPYTLRIAAKEGRLVLDIEPVQGGPGATHIVPLASLRNLLKDYLLICDSYYA
                     AIRTATPAQIEALDHSRRAAHDEASAVLAERLAGKIEIDKETARRLFTLIVALHAKA"
     gene            complement(8626..9924)
                     /gene="hisD"
                     /locus_tag="VKU03_13765"
     CDS             complement(8626..9924)
                     /gene="hisD"
                     /locus_tag="VKU03_13765"
                     /EC_number="1.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004399 - histidinol dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidinol dehydrogenase"
                     /protein_id="HLJ72382.1"
                     /translation="MTFRLDMSAADFGPQFALLLGSKREASQDVDAVVAGIIEDVRKR
                     GDEALAEYSLRFDRIDINRLGLRVGDSEIGAAIEACDRHALAALELAHRRVRAYHERQ
                     KPQDVSFADALGVELGWRWRPIEEVGLYVPGGGAAYPSSVVMNAVPAKVAGSARVVMT
                     VPAPDGKINPLVLAAARLAGVDEIYRVGGAQAIAALAYGTASIRPVAKIVGPGNAYVA
                     AAKRRVFGLVGIDMIAGPSEVVILADSSADPDFVAADLLAQAEHDEAAQSILITDDRA
                     LAETVTAAVERQLAALPRAAIAGASWRDFGAIILVPSLAAAVPLVNRLAPEHLEIMAR
                     DAESLSGRIANAGAIFIGAATPEAIGDYVGGSNHVLPTARSARFSSGLGVLDFMKRTS
                     LLKCDPQSLKALGPAAIALGEAEGLSAHARSVALRLERGG"
     gene            complement(9948..10406)
                     /locus_tag="VKU03_13770"
     CDS             complement(9948..10406)
                     /locus_tag="VKU03_13770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2948 family protein"
                     /protein_id="HLJ72383.1"
                     /translation="MSEAATDLKLIALDGEDLAVISAHVQDAFVRRGEIAYLSGQKRF
                     ALGALRYDWAGSVAGRKERVGAVLRFDRVLKVSQTGLAGLKPDAMLNLLAVSFDENDP
                     PAGIVTLTFSGGAAIRLHVECLESELRDIGPRRPVKACPGHALANEGETG"
     gene            complement(10409..11698)
                     /gene="murA"
                     /locus_tag="VKU03_13775"
     CDS             complement(10409..11698)
                     /gene="murA"
                     /locus_tag="VKU03_13775"
                     /EC_number="2.5.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008760 - UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine
                     1-carboxyvinyltransferase"
                     /protein_id="HLJ72384.1"
                     /translation="MDRISIKGGARLNGEIRISGAKNAALPLMIASLLTEETLTLENL
                     PRLADVSMLVRILGNHGVDYSLNGKRAGEALDSGQTAHLTAREIVDTTAPYDLVSKMR
                     ASFWVIAPLLARAGEARVSLPGGCAIGTRPVDLLLMALENLGAKIEIDAGYVVATTPR
                     GFIGGEISFPKVTVGGTHTAMMAAATAHGSTLIRNAAREPEVKDLADCLNKMGARIHG
                     AGTATIEVTGVARLNGTRHSVLPDRIETGTYATAVAMTGGDVLLKGAVADHLQEALDA
                     LNAAGASVVSDNQGVRVKRNGAGISPVDIETAPFPGFATDLQAQFMALMTRAKGVSRI
                     RETIFENRFMHVQELARLGAHIKLEGDTAIVEGVGKLRGAPVMATDLRASVSLVIAAL
                     AAEGETIVNRVYHLDRGFENLEHKLGACGAEVKRISG"
     gene            complement(11740..11910)
                     /locus_tag="VKU03_13780"
     CDS             complement(11740..11910)
                     /locus_tag="VKU03_13780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72385.1"
                     /translation="MTSDKGREREAETKRRRLALALRENLKRRKAQQRGRQEAVAPNA
                     SPDVRPGDEQKI"
     gene            complement(12033..13703)
                     /gene="recN"
                     /locus_tag="VKU03_13785"
     CDS             complement(12033..13703)
                     /gene="recN"
                     /locus_tag="VKU03_13785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265678.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecN"
                     /protein_id="HLJ72386.1"
                     /translation="MLVRLTIRDIVLIDRLDLEFGPGLSILTGETGAGKSILLDSLAL
                     ALGARGDGALVRQGQSQGQVTAVFDVSPDLPALAQAREQGVAVDGELIMRRVQLADGR
                     TRAFVNDLPVGVQTLRTLGAGLVEIHGQHDERALIDAASHRALVDAFGGLAEEAAEAR
                     RAYGALKESEKRLAAETERLKAARADADFLRHAHEELKRLAPLAGEEAMLAERRAAMM
                     QSEKVVADIQAAHESLAGDAALAQRIAAAARRLQRRAVQAPALVEPCVKALDAALEAL
                     DAAQSAVEEALRAAEFDPKDLERAEERLFALRAAARKYSVPVDNLATLAETYAADLAA
                     LDAGEARLAALEKAAAEAARAYAKAAAALSRARARSARDLERAVNAELAPLKLERAEF
                     SVMIASDPANAAPEGIDRIEFHVRTNPGSRAGPLMKVASGGELARFMLALKVALADRG
                     SAPTLVFDEIDTGVGGAIADAIGQRLARLAARAQVLAVTHAPQVAARSTSHFRIAKAA
                     IGKGERVATSVAALDEKARREEIARMLAGATITEEARAAARRLIESAG"
     gene            complement(13722..14606)
                     /locus_tag="VKU03_13790"
     CDS             complement(13722..14606)
                     /locus_tag="VKU03_13790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265679.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamD"
                     /protein_id="HLJ72387.1"
                     /translation="MHFAFLPITSKGRAARLAAAILLAAPAAACGSFDINDYNPFGAE
                     KYKMKVEPDVPASQIYDQGLARLAKEDGSGAAKKFVDLGKNYPDSEWSKKALLMTAYS
                     QFKAGEYDNAEQSAEKYVKLYPESSDAAYAAYLEASSYYAQIPDISRDQDRTAKALEL
                     FSQIVQKYPNSEYVNDSKFKIQVARDQLAGKEMSVGRFYLHRRNYVAAINRFRNVLAN
                     YQTTRHAEEALYRLTEAYLGLGVTSEAQTAAAVLGHNFPDSQWYKDAYALLKGGGLEP
                     EENKDSWISKIYHTVVPG"
     gene            complement(14816..15766)
                     /gene="lpxC"
                     /locus_tag="VKU03_13795"
     CDS             complement(14816..15766)
                     /gene="lpxC"
                     /locus_tag="VKU03_13795"
                     /EC_number="3.5.1.108"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008759 - UDP-3-O-[3-hydroxymyristoyl]
                     N-acetylglucosamine deacetylase activity [Evidence IEA];
                     GO_process: GO:0009245 - lipid A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-3-O-acyl-N-acetylglucosamine deacetylase"
                     /protein_id="HLJ72388.1"
                     /translation="MKLGRQTTLRERVTISGVGVHSNEPVTVILHPADANSGVVFLRS
                     GLPGGRERLIEAKWSNVAQTQLCTVLGDPAKASVATVEHLLAALAGLGVDNVTIEIDG
                     PEAPILDGSAAPFVAAIDEVGLASFSAPRRYLKILKPVRVERGDAYAELRPAERALRL
                     DVEIDFATPAIGRQRKIFDLDAGAFRREIARARTFGFVGDVERLWKMGFALGSSLDNS
                     VALDGDRVVNPEGLRYRDEFVRHKMLDSIGDLALAGAPILGAFKAYRPGHAMNLAALQ
                     TLFADRSAYEFIEAPRHAPTPAHAVLSGRLPVAAYAAEVD"
     gene            complement(16045..17709)
                     /gene="ftsZ"
                     /locus_tag="VKU03_13800"
     CDS             complement(16045..17709)
                     /gene="ftsZ"
                     /locus_tag="VKU03_13800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HLJ72389.1"
                     /translation="MTINLKAPELRELKPRIMVCGVGGAGGNAVNNMIVSGLIGVDFI
                     VANTDAQALTSSKADRIIQMGLQVTEGLGAGSKPEVGRAAAEEAIEEIRDHLAGAHMV
                     FVTAGMGGGTGTGAAPVVARVAREMGILTVGVVTKPFQFEGARRMRVAEAGIEELQKC
                     VDTLIIIPNQNLFRVANEKTTFADAFAMADQVLYSGVACITDLMVKEGLINLDFADVR
                     SIMSEMGKAMMGTGEASGERRAIQAAEAAIANPLLDEVSMGGARGLLISITGGNDLGL
                     YEVDEAASRIRQEVDEDANIILGATFDQSLDGVVRVSVVATGIDQDKVEAKEPSASQQ
                     RIAEAAQRLRAQAAARLHAAAAAPAPAAPPPPQPIVEAPVEMAPVAAPVLPAAAEVEL
                     RPAAPKPAFYVEPATQQTLFEEPASEFVPPAPEQIVRPARMPQIEDLPPVVQQQMRIH
                     RGEQPAPAESRRKSLLEKLAAFGITRHEETGPPAPRAAPVLPAPPVRAAPPPQSHADY
                     GRPQPRALAPRVAQGALDPHGRPAPKTTAFEEDQLEIPAFLRRQSS"
     gene            complement(17813..19129)
                     /gene="ftsA"
                     /locus_tag="VKU03_13805"
     CDS             complement(17813..19129)
                     /gene="ftsA"
                     /locus_tag="VKU03_13805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383705.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HLJ72390.1"
                     /translation="MSFKPVTPRLRQIPPRKSAVLSVLDVGAAKIVCLIARLTPMPAS
                     DSLRGRTHRCKVLGIGHQRSRGVKAGAIVDLDAAETAMRLAIDAAERMAGVEVESIIV
                     NMTGGRLASQFYNARVAIAHKAVSEHDIHRVLEAASHASAPAGRIALHALPMGFSLDA
                     TRGVRDPKGMVAHELGAELHVASCDGAAARNLMLAVERCHLRVDAVVATPYAAGLSTL
                     VDDEAELGSALIDFGGGSTSIGVFVGGRLAHVDAVAVGGNHITTDIARGLAVSLADAE
                     RLKTLYGSCIASPSDERDTMTVARVGDDLDHPSVLPKSELVRIIRPRVEEILELVRDR
                     LKQAGHAAHAGRRLVLTGGACQLTGLPELARRIISSQIRIGRPLGVDGLPESAKSPAF
                     AAAVGLMVYPQVAGIEHFEPGRRSYSSSAAGDGYVARVGRWLKESF"
     gene            complement(19126..20073)
                     /locus_tag="VKU03_13810"
     CDS             complement(19126..20073)
                     /locus_tag="VKU03_13810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HLJ72391.1"
                     /translation="MRPIAEAFALGGLSAPLAPAAIGAGEIRAPLVLLRGERSAGRGL
                     TRLRRFLLSAPRGLGLALAVALLGGVAAFGAVRGGEYQAFIAREGDLRDVIARALGFD
                     IAIVTISGQVELREREILAAAHITPKDSLLFLDAAAARKDLEALPLVKSASVRKLYPN
                     HLVIDLVERAPYALWQKDGKVAIVAADGAPIDRMTDDRFIGLPFVVGDGANERLPEFM
                     SILDAAGELRPKIKAGVLVSGRRWDLAMTNGMVVKLPEADPEAAMATLVRLQRTDRVL
                     ERAALSLDFRVPGKMFVRLTEDAAAARAAAARPAKKGAT"
     gene            complement(20061..20981)
                     /locus_tag="VKU03_13815"
     CDS             complement(20061..20981)
                     /locus_tag="VKU03_13815"
                     /EC_number="6.3.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase"
                     /protein_id="HLJ72392.1"
                     /translation="MKHVAVLMGGWSGEREVSLRSGAGCAAALESAGYRVTRVDVQRD
                     IAEVLAKLKPDVAFNALHGRFGEDGTIQGVLEILSIPYTHSGVLASALALQKDLAKTV
                     MAAAGIPVAHGLVVDRHDAAKRHALEPPYVLKPLYEGSSLGVVIVKQGRSHPPQEVGR
                     PDWPYGDVLLAERYVAGLELTCAVMGDRVFDVIEVQPVSEAFYGYDAKYAKGGSIHVL
                     PAKIKPNIYHDIQQLSLRAHRALGCRGVSRADFRYDEAKGEKGELVCLEVNTQPGMTE
                     TSLVPEMAAYAGISFAELVSWMVEDASCDR"
BASE COUNT         3402 a         7061 c         7208 g         3510 t
ORIGIN      
        1 aacatgctgt accaagtgct gctcggcgag gaacgcggac cgcgcttcgg ctcgttcgtc
       61 gccctctatg ggatcgagga gacgcgcgcg ctgatcgccg cggcgctgag cggagcgctg
      121 gtcagagagg ggccgccagc ataagcttcg tccccctcgc tgcgctcggg atccgcgcaa
      181 tggcggggta tgtacgaagc aggcgttcgt cggtcttcgc cgctttacct tcgcttgccg
      241 gcgctcagcg gtggcggcaa ctggtcgatt ggattaagga cgggtccgcc gaggggctcg
      301 aaatgtcgta tgtttcgagt caggatcgtg agggcgtgag tttgagcggt cgccgcgatt
      361 acgacatcgg caaagcccgg ctcatgtccg gcgctccttg caatatctat gatcttgccc
      421 gcgaccgctg ccgtcctcag atcgaatgga aggatccgag cgccataaag atgctcgacg
      481 ctgtcccacc agtcccttag gagggacgct ttgcgggctg cgcccaggcg cgccgccttc
      541 gcgatcccag cctgtatttc agcagcggtg gcggccgaga gataaagtcc gctggacgcg
      601 tcatccaacc agcgcgccag cgcggccggg cgatcggatt ttgtcggcgc gacggccgag
      661 acgatgtcgg tatcgagaag atacgtcaaa gatcaacgtc gcgagagggc gatcgatcac
      721 gtgctggaat gtcgccttcc tcaaggggag aggaagcgag caggcggcca aagctaggaa
      781 cattcgacaa gcgccgccat gtgtcccagg aaatcacgac ggcttgcttc cgcccgtggc
      841 gcgtgatcgc gaacgcctcg ccattcaacg tttcatcgac gatggcggag agtgtcgcct
      901 tcgcgtcttt gagctgaatt tcacgcatag ccgtatcgtg ctaaaaatga ctaatatagt
      961 catattaccg cttgccgggc ttgtccagtg cgctcctcac tccagcgccg ttccattcgc
     1021 ctctagcgta gcgccggcga ggtagagcga gccggcgatc agcacgcgtg gcggcgccgg
     1081 ccagcttcgc gccgagagga agcgcaaagc cgcttcgacg cccggcgagg cggccgcttc
     1141 gatgccgacg ctcgcggcga tctgggcgac ctcgtcggcg cttcggccgg catgctcgcc
     1201 ggcgatcggc acggcgagga gctcctgcgc cagccccttg aaatggcgca ggaagccggc
     1261 cgtatccttg gtcgccagcg tgccgcagat cacgaccagc ggccgcggcg actgctcttc
     1321 gaaatcggcc atggccgcgg cgaggacgcg accgccgtct tcgttatggc cgccgtcgag
     1381 ccaaagctcg gcgcgggcgg gcgcaaggtc gaccagcttg ccgctgcgca ggcgctgcag
     1441 gcgcgccggc cattcggcct cggtgaggcc gcgctcgaac gcgcgaaggc tgatatgcgg
     1501 ctcgatcctg cgaagcgccg ccaccgcatt ggcggcgttg ccatgctgat ggcggcccgg
     1561 caggcgcggc agcggcagat cgatcaggcc gagttcgtcc tcgaagacga gccggccatt
     1621 ctcctcgcgc aaggaaaaat cgcggccatt gacgatcagt tccgcgccga cccgctgcgc
     1681 ttcgcgctcg atgacggcaa gagcgcgcgc gtcctgttct gcgatgatcg ccggcgcgcc
     1741 gcgcttcagg acgcccgcct tctcgtaggc gatcttctcg acggtcgcgc cgaggaattc
     1801 aacatggtcg atcgagatcg acgtgatgat cgccgccgcc ggcttgtcga tcacattggt
     1861 cgcatcgtac cgtccgccga gcccgacttc gagcagcagg cagtcggccg gcgcctcgct
     1921 gaacagcagc agcgccgccg cggtggtgat ttcgaacacc gtgatcgcct ggccgccatt
     1981 gacccgctcg cagcgatcga acgcctcggc cagccgctcg tcgtcgacca gcttgccggc
     2041 aaggcggatg cgctcgttga agcgcaccag atgcggcgag gtgtagacat ggacgcgttt
     2101 gcccgccgcc tccagcgccg cgcgcatgaa ggcgatggtc gagcccttgc cattcgtgcc
     2161 ggcgacatgg acgaccggcg gcagccgctt ctccggcgcg ccgagatcgg ccagcaagcg
     2221 ctcgatgcgg ccaagcgaaa ggtcgatctt cttcggatga agcggggcga aacgcgtcag
     2281 aatcgcgtcg tgcggattca tgggcggctt cgctcccaag gcgccgctca agccgccgcc
     2341 gcggccgcgc cggacgtcag cagcttgcag agccgcgcca aaaccggccg caattggtgg
     2401 cgcggcacga ccatgtcgac catgccgtgc ttcttgagat attcggcgcg ctggaatcct
     2461 tccggcaggc gctcgcggat cgtctgctcg atcacccgcg cgccggcgaa gccgatcacg
     2521 gcgccaggct ccgcgatatg gatgtcgccc agcatagcgt aagaagcggt gacgccgccg
     2581 gtcgtcggat tggttaggac gacaatgtag ggaagcttcg cggcgcgcag gcgctgcacc
     2641 gcgaccgtgg tgcgcggcat ctgcatcagc gagaacatgc cctcctgcat gcgcgcgccg
     2701 cccgaggcgg tgaagatgat gaagggcgtc cgccgctcga ccgcggcggt catgccggcg
     2761 atgatcgcct cgcccgccgc catgccgagc gtgccgccct ggaaggcgaa atcctgcacc
     2821 gcgacggtga cggtcaggcc ttcgagcccg ccgacggcga ggatgaccgc gtcggccgag
     2881 ccggtcttgg tccgatagtc cttcagccga tcgatgtagc gcttgacgtc gcggaatttg
     2941 agcggatcgg cgggcacttc cggcgtcggc aattcctcgt attcgccgcc gtcgaagaga
     3001 ttggcgagcc gcgccttcgc cccgatcgcc atgtgatagc cggagcccgg cacgacgaag
     3061 agattggctt cgaggtcttt gtggaagacg agttgcccgc tttccgggca tttgacccag
     3121 agattctccg gcgtgtcgcg cctgaggaag gagcggattt tcggcggaac gacatccgac
     3181 atccagttca tcgaaaagct gctcctgatc ttaagccgaa accttttgtt tcgccccgcg
     3241 cacgcccgcc gccaattcct ggaccagggc ggtcaccgcg ccgacggttc cggcggtcgc
     3301 cttgccgcca ttcagcgagg tccggacagc ctcgaccaga gccgagccga ccgccacgcc
     3361 atcggccttg gcggcgatcg ccgcggcgct ggcggcgttc ttgacgccga atcccaccgc
     3421 cacaggcaaa tggctatgcg ctttgagccg cgcaaccgcc gccccgacct tgccggcgtc
     3481 gggcgcggcc gcgccggtta ttcccgtaac cgagacgtaa tacacgaaac cggacgtgtt
     3541 ggcgagaacc gccggcaggc gcttctcgtc ggtggtcggc gtcgccatgc ggatgaaatt
     3601 cagcccggcc ttgagcgccg gcaggcacag ctcctcgtcc tcctccggcg gcaggtcgac
     3661 gacgatcagg ccgtcgacgc cggcggcctt ggcgtcgtgc aaaaaggctt cgacgccatg
     3721 gacatagatc gggttgtaat agcccatcag gaccatcggc gtcgccggat cggctttgcg
     3781 gaattccgcg accatggcca gcgtcttgcg catggtctgg ccgccattga gcgcgcggag
     3841 gccagaggcc tggaccgccg gcccgtcggc catcggatcg gtgaacggca tgccgagctc
     3901 gatcacgtcc gccccggcgc caggcagcgc cttgagaagg gcggtcgagg tctcgaggtc
     3961 cgggtcgccg gcggtgatga acgtcaccaa ggccgcgcgg ccttcggcct tgagagcggc
     4021 aaaacgagcg tcgatgcggg tcgtcatcgg ggcgaggtag catgagcggc ggccagaggg
     4081 aaccgcagcc gtgcgatggg ctcatctgct cgcgcgatgg cggccgccaa ggaacatgtc
     4141 ttgccgggag aatgttgagc cagggccagg ggctctgtcg cggtgcgtcg tcttggcatt
     4201 tcagaacatt tcgctgggcg ccgggcggcg cggccttgcc gggcttgtcg gccgaccggg
     4261 cggcattcgc cggccggcgg cgtttttcat catcatctgc atcgtcgcgg gcgtgctcgc
     4321 gccggccttc ttctcgctca acaatttcat cgcgctgctg accaattcga gcttcctcat
     4381 cgtcgtcgcg gcgggagaat ccttggtcat cctggtcggc atgatcgatc tcggcgtcga
     4441 gagcgtgctc gccgcctccg gcatgttcat cgcgtggctg accgtgtttc acggcgttcc
     4501 ggcgggcgcg gcaatcgtcc tgacgctcgc cgccggcgcc gcggtcggcg tcttggtcgg
     4561 cctgctcgtc gcgcgcggcg gcattccatc cttcatcgtc acgctcgcca cctattgggg
     4621 cctcagaggc gtcgcgctgc tgttcaacgg cggcaattac atcagtcccg atgcggtcag
     4681 cccgccgcga cctttcggct ttgccggaat cgccgccaac attggcggcg tccccattct
     4741 gattctcatc gcgctgatta tcgtcattct cgcgcaagcc gtggtttcgc tgacgccgct
     4801 cggcgcctgg atcagggcgt cgggctcgaa tgagaacgcc gcccgcgccg tcggcgtcag
     4861 aaccgacgcc atcaagattc tgaccttcgc gatttccggc ttcatggccg cgctcgccgg
     4921 aatcatgatc accgcctggc aagattcgat ctatccgctc accggcgagg gctattcgct
     4981 gcaagcgatc gccggcgtga tcctcggcgg gattccgttc aatggaggcc gcggcacgat
     5041 cgtcggcgcg gcgctcggcg tgctggtcat cggcgtgatc aacgacgtca tcgtcctgat
     5101 cggcttgccg gctttgtacg aatatatttt cgtcgccgcg atcctggttc tcgccggcct
     5161 gcaggcgcgg ctggcctcgg cgaagtagcc gcagcgacga cgcaatccct tcgtagagcg
     5221 cggagcctat gaatggatcg cgttgcttcg cttcgctcgc aatgacggcg gatcgcgtat
     5281 tcgcgtcaaa acatcgcgtg aaccaggtcg ccgaccgaga ggcccttgtt ctccaggatc
     5341 gtccgcactt tgccttgacg gaacacgacg atgcggtcgc agacctgaag caggtcctcg
     5401 agccggtggc tgacgaggag caccgtcttg ccggcggcgc gcagccgcgc gatcagggcg
     5461 agaacgtcgc cgactttctg caccgagatc gccgccgtcg gctcgtccat gatgatgatc
     5521 tggcgctcga atagaatggc gcggccgatc gccaccgctt gctgctcgcc gccggaaagc
     5581 tcgccgacaa tggcctccgg caggattttc gtattcaatt gcttgaggac gccggccgcc
     5641 tgctcgcgca tcaggcgacg cgccagccag acgccgaagc ggcgaagctc cgcgccgagg
     5701 aagaggtttt cccaaacttt caggtcgggc gcgagttcga gccgctgata gatcatctcg
     5761 atcttgagat cggcccgcgc ggtgcgcggc gagcgcaccc gaacctcgcg gccgcgatag
     5821 aggaagcggc cagaatccgg gtgatggaac ccggtgagca gggcgaggaa ggtcgacttg
     5881 ccggcgccgt tatcgccgac aatgccgacg acctctcccg gatagaaatc gaggctcacc
     5941 ccgtcgacgg cgcggaccgg cccgaaggtc ttgaccacgc cgcggacgga aaggagcggc
     6001 ggcggggcgg ccgccgccgt ctcggcgctc atttgccgct tgtcaccttg aaggcttgcg
     6061 cctggccgta gggcagaggc gggatctcct ccggcgtgcc ccaggcggcg atctggccga
     6121 cattggcctt atcgaccagc gtcatcggga tgatgatgtc gccatgcggg aatttcgcct
     6181 ccggcggctt ctcgccgttc ttcagatagg ccgacatcgc ctcgaccgcc caataggctt
     6241 cgacgaaggg cgcatgggtc accgtgtcga gctgcgttcc cgccttgatg gcgtccatgc
     6301 tctcgggctg cgcgtcggcg ccgacgacat aggtcttttc gcccggcgtg acattatgcg
     6361 ccttcaaggc ctggatgacg ccgagcgcca tggcgtcgtt gccgcagacg acgagctgga
     6421 tattggtcgt cttggcgagc tcggccgaca tcatcgcctg cgccttgtcg ctggcgaaat
     6481 cggcggactg gttgaggacg acatcgacct ccttcttgtc gatcagaggc tgcagcacgt
     6541 ccatcgcgcc tttcaggcgg ccgatcgaga cgctggcgcc gagggtgccc tggagaatgg
     6601 cgacatgcca gggcttcgga accttggcct tggccagcgt atcgacgcca tattgcgtct
     6661 cctcgcggcc gagcgtctcg tcattgtcgc cgacaaaggc gacgcgcgca tcgggccggc
     6721 tcacatcgcg gtcgacggtg acgatcggca cgccgccttt catcgcgcgc tcggccgtgg
     6781 cggtgaccga ttcctggatc gtgttgagga tgatcccctt tgcgccggcg gcgaccgcct
     6841 cgttgatgtc ggcgatctcc tgcgaggccg aattgttcgc gttgacgatc ttgatcgtca
     6901 tgtcgggata atgcgccgcg gcctgcttca ccgcctcggc catcggcgcg aaatagggat
     6961 aggcgtagcc ggtgaagatg aaatagacgg gcttcggcgc ctcggaagag gccgtcgcgg
     7021 cgagggctgg ggcggcgccc gcggcaagcg cggcgaggca gacggcagtc agggttttca
     7081 tggagaatcc tccctttctc gcccctgaga tgcgaaaatc aacgctccga aaagggctgc
     7141 gttccaaggc gcgcaaaaaa gggagggcgc gggccccgcg ctgaatcgtc gccatggccg
     7201 gatttatcgg cccttgattg ggcggcgccg cagcgcggcg gccatgcggt tatcgaaacc
     7261 gatacaccgc cgtccgcttg atctccgcat cctccagagc tcgcgtcgcc ggcacacgat
     7321 attcagcgat cggctccagc cggtcgcggg cgaggtacgc gcggcccgga tcgccgatca
     7381 gaacctcggc ccggcgcttg gccaagctcg cgagccaatc ggtaaccgcc tcggccatgt
     7441 ctttctggta cgacacatcg ccggccagga ccacgtccca gccttcgtcc gtcccgacga
     7501 gatcggcgag ccgcggcgca acctgaacgc cgttcagcgc cgcgttgagc gcgatcgcct
     7561 cgacggcgaa ttcgtcgatt tcgcaggcct cgacgctcgc cgcgccgccc atagccgccg
     7621 cgctgccgac aagaccggag ccggcggcga aatcgaggac gcgcttgccg gcgacgacgc
     7681 ggcgatgatc gagaagatag cgcgccagcg cctggccgcc ggcccaagcg aacgcccaga
     7741 aaggcggcgg caagccgatc cggccaagct cctcctcggt cttcttccac agcgccgtcg
     7801 cctcgtcggc aagatgcagc ttgatttcgg gcgcatgcgg gacgggaagg acgcgggtat
     7861 tggcgaggat gaacgcgcgg cggtcggcga tcgtcactcg tcacgccttc gcatgcagag
     7921 cgacgatcag ggtgaacagc cgccgcgccg tctccttgtc gatctcgatc ttgccggcga
     7981 ggcgctcggc gagcacggct gaggcttcat catgcgcggc gcggcgcgaa tggtccaagg
     8041 cttcgatctg ggccggcgtc gcggttcgga tcgctgcata atagctgtcg cagatcagga
     8101 gatagtcctt cagcaaattg cgtagcgaag cgagcggcac gatatgcgtc gcgcccggcc
     8161 cgccctggac tggctcgata tcgagaacca gccgcccctc cttcgcggca atgcgcagcg
     8221 tgtacggccc gtcgtcgcgg tccggcaggc cgaatgaatt ctcctggaga aggtcgtaga
     8281 tcgcgatcgc ccgctcatgc tcctggtcgg gcgtgccgcg ggcgagcggg tcgtcgagcg
     8341 cgacgtcgac caaccggcgg cgatccgcag gcgcgctcat gagagcacgc cgtcgctatg
     8401 tcgggctgac gagcgtgaga aaagtctcgg cgtcaggggc ccctctcctc gtggagcgaa
     8461 gcggaacgga gagaggtaag gagaggggct ggtcgagtcg ccccgaccgc cgaagctcag
     8521 cgacccgccc ctctcggtac ctctccccgc tgcgcgggga gagggggctt cgacgccggg
     8581 tctcgtcttc aaacgccagc gctggcgaca ccgagcgtcc atcgtctagc cgccccgctc
     8641 cagccgcagc gcgacggagc gcgcatgcgc gctgaggccc tcggcctcgc cgagggcgat
     8701 cgcggccggg ccgagcgcct tcagcgattg cggatcgcat ttgagcagcg aggtccgctt
     8761 catgaaatcg agcacgccga ggccggagga gaagcgcgcc gagcgcgcgg tcggcaggac
     8821 gtggttggag ccgccgacat agtcgccgat cgcctccggc gtcgcggcgc cgatgaaaat
     8881 cgcgccggca ttggcgatgc ggccgctcaa agactcggcg tcgcgcgcca tgatttccag
     8941 atgctccggc gccagccgat tgacgagcgg gacggctgcg gcgagcgaag ggacgagaat
     9001 gatcgcgccg aaatcgcgcc agctcgcgcc ggcgatcgcc gcgcgcggca gagccgcaag
     9061 ctgccgctcg accgctgcag tgacggtctc cgccagcgct ctatcgtcgg tgatgaggat
     9121 cgactgcgcc gcctcgtcat gctcggcttg cgcgagaaga tcggcggcga cgaaatccgg
     9181 atcggccgag gaatcggcga ggatgacgac ttccgaaggg ccggcgatca tgtcgatgcc
     9241 gaccaggccg aagacccggc gcttcgctgc cgcgacatag gcgttgccgg gcccgacgat
     9301 cttggcgacc ggcctgatcg aggccgtgcc ataggcgagg gcggcgatcg cctgggcgcc
     9361 gccgacgcga tagatttcgt cgacgcccgc gagcctggcg gcggcgagga ccagcgggtt
     9421 gatcttgccg tccggcgccg gcaccgtcat cacgacgcgc gccgagccgg cgaccttggc
     9481 ggggaccgca ttcatcacca ccgaggacgg ataggccgcg ccgccgcccg gcacgtacaa
     9541 gccgacctcc tcgatcggcc gccagcgcca gccgagctcg acgccgagcg cgtcggcgaa
     9601 ggagacatcc tgcggcttct ggcgctcgtg ataggcgcgg acgcgccgat gggcgagctc
     9661 cagcgccgcc agcgcatggc ggtcgcaagc ctcgatcgcg gcgccgatct cgctgtcgcc
     9721 gacgcgcagg ccgaggcggt tgatgtcgat gcggtcgaag cgcagcgaat attcggcgag
     9781 cgcctcgtcg ccccgcttgc gcacgtcctc gatgatcccg gcgaccacgg cgtcgacatc
     9841 ttgcgaggct tcgcgcttgg agccgagcaa aagcgcgaat tgcggtccga aatcggccgc
     9901 gctcatatcg aggcggaacg tcatggcttt tctccgggcg gcgaggctta gcccgtctcg
     9961 ccctcattcg ccagagcatg gcccggacag gccttgaccg gccggcgcgg gccgatgtcg
    10021 cggagctcgc tttcaaggca ttcgacgtgc agacggatcg ccgcgccgcc ggagaaggtc
    10081 aaggtgacga ttccggccgg cggatcgttc tcatcgaagc tgaccgccag gagattgagc
    10141 atcgcgtcgg gcttcaagcc ggcaaggccg gtctgcgaga ctttcaggac gcggtcgaag
    10201 cgcaacacag cgccgacccg ctccttgcgg cccgccacgg agcccgccca atcgtagcgc
    10261 aaggcgccga gcgcaaagcg cttctggccg gaaagatagg cgatctcgcc gcggcgcacg
    10321 aaagcgtcct gaacatgcgc ggagatcacg gcgagatcct cgccgtcaag cgcgatcagc
    10381 ttcagatcgg tcgccgcctc gctcatcgtc atcccgatat gcgcttgacc tcggcgccgc
    10441 aggcgccgag cttgtgttcg agattctcga agccgcggtc gagatgatag acgcggttga
    10501 cgatcgtctc gccctcggcc gccaaggcgg cgatgaccag cgagaccgag gcgcgcaaat
    10561 ccgtcgccat gaccggcgcg ccgcgcaatt tgccgacgcc ttcgacaatc gcggtgtcgc
    10621 cttcgagctt gatatgggcg ccgagccgcg ccagctcctg cacatgcatg aagcggtttt
    10681 cgaaaatcgt ctcgcggatg cgcgagacgc ccttggccct ggtcatcagc gccatgaatt
    10741 gcgcctgcaa atcggtggcg aaaccgggaa acggcgccgt ctcgatatcg accgggctga
    10801 tcccggcgcc gttgcgcttg acgcgcacgc cctgattgtc gctgacgacg ctcgctccgg
    10861 ccgcgttgag ggcgtcgagc gcctcttgca ggtgatcggc gaccgcgcct ttaagcagca
    10921 cgtcgccgcc ggtcatggcg accgcggtcg cgtaggtgcc ggtctcgatc cggtccggca
    10981 ggaccgaatg gcgcgttccg ttgagcctgg cgacgccggt cacctcgatt gtcgccgtgc
    11041 cggcgccgtg aatgcgcgcg cccatcttgt tgaggcaatc ggcgaggtcc ttgacctccg
    11101 gctcgcgcgc cgcgttgcgg atcaaggtcg agccatgcgc cgtcgcggcg gccatcatcg
    11161 ccgtatgggt gccgccgacc gtcactttcg ggaaggaaat ctcgccgccg ataaagccgc
    11221 gcggcgtggt ggcgacgaca tagccggcgt cgatctcgat cttcgcgccg agattctcca
    11281 gcgccatcaa caggagatcg accggcctgg tgccgatggc gcagccgccg ggcagcgaga
    11341 cgcgcgcttc gccggcgcgc gccagaagcg gcgcgatcac ccagaagctc gcccgcattt
    11401 tcgagacgag atcgtaaggg gcggtcgtgt cgacgatttc gcgcgccgtc agatgcgcgg
    11461 tttggccgga atcgagcgct tcgccggccc gtttgccgtt cagcgaatag tcgacgccgt
    11521 gattgccgag aatgcgcacc agcatgctga catcggcgag ccgcggcaag ttttcgagcg
    11581 tcagcgtctc ctcagtcagg aggctggcga tcatcaacgg caaggcggcg tttttggcgc
    11641 cagaaatccg gatctcgccg ttcagccgcg ctccgccctt gatgctaatg cgatccatgc
    11701 tggcccctgt tccgcgcctt gagacggcgc gattgacgtt cagatttttt gctcatcgcc
    11761 gggccggacg tccggcgaag cgttcggcgc aacggcctct tgccggccgc gctgctgcgc
    11821 cttgcggcgc ttgaggttct cccgcaaggc caaggccaga cgccggcgct tcgtctcggc
    11881 ctcacgctcg cgtcccttgt ccgacgtcat ttcggctccc ctgcagcctc taaggccgcg
    11941 tcacggctag ataggcgcgg cggcacagcg gagcaagccg caaggccgcg ccatccccgc
    12001 caacggcggg ccggcgcgcc tgttcccaaa aatcaccccg cgctctcgat cagcctgcgc
    12061 gccgccgcgc gcgcctcttc ggtgatcgtc gcgccggcca gcatgcgggc aatctcttcg
    12121 cgccgcgcct tttcgtcgag ggcggcgacg ctggtcgcga cccgctcgcc tttgccgatc
    12181 gccgccttgg cgatgcggaa atggctggtc gagcgcgcgg cgacctgcgg cgcatgggtg
    12241 acggcgagca cttgcgcgcg cgccgccagc cgggcgaggc gctggccgat cgcgtcggcg
    12301 atcgccccgc cgacgccggt gtcgatctcg tcgaagacga gggtcggggc cgagccgcga
    12361 tcggccagcg ccactttgag cgccagcatg aaccgggcga gctcgccgcc cgaggcgact
    12421 ttcatcaacg gaccggcgcg cgagccggga ttggtgcgca cgtggaactc gattcgatcg
    12481 atcccttccg gcgcggcgtt ggccggatcg ctggcgatca tcaccgagaa ttcggcccgc
    12541 tccagtttca gcggcgccaa ttcggcgttg accgcgcgct ccaaatcgcg cgcgctgcgc
    12601 gcgcgcgccc tagaaagcgc cgcggcggcc ttggcgtagg cgcgggcggc ttcggcggcc
    12661 gccttttcaa gcgcggcgag cctcgcctcg cccgcgtcga gagcggcaag atcggcggcg
    12721 taagtttcgg ccaaggtggc aagattatcg accggaaccg aatatttgcg cgccgcggcg
    12781 cgcaaggcga agagccgctc ctcggcgcgc tccaagtcct tcggatcgaa ttcggcggcg
    12841 cgcagcgcct cttcgaccgc gctttgcgcg gcgtcgagcg cttcgagcgc cgcgtcgagc
    12901 gccttgacgc agggctcgac tagcgccggc gcctggaccg cgcgacgctg caggcgccgc
    12961 gccgcggcgg cgatgcgttg cgccaaagcc gcgtcgccgg ccaggctttc atgcgccgcc
    13021 tggatgtcgg cgacgacctt ttccgattgc atcatggcgg cgcggcgctc ggccagcatg
    13081 gcctcctcgc cggccagcgg cgccaggcgc ttcaattctt catgggcatg gcggaggaaa
    13141 tcggcgtcgg cgcgggcggc cttcaggcgc tcggtctcgg ccgccagtcg cttttcgctc
    13201 tctttgaggg cgccgtatgc gcggcgcgcc tccgccgcct cctcggcgag gccgccgaac
    13261 gcgtcgacca gggcgcgatg gctcgccgcg tcgatcagcg cgcgctcgtc atgctggccg
    13321 tgaatctcga cgaggcccgc cccgagcgtg cgcaaggtct ggacgccgac cggcaagtcg
    13381 ttgacgaaag cgcgagtgcg gccgtcggcc aattgcacgc ggcgcatgat caactcgccg
    13441 tcgaccgcaa cgccttgctc gcgcgcctgg gcgagcgccg gcagatcggg ggaaacgtcg
    13501 aacaccgccg tcacctggcc ttggctctgg ccctgtcgaa ccagcgcgcc gtcgccgcgc
    13561 gcgccaagcg ccagcgccag ggagtcgaga aggatggatt ttccggcgcc ggtttcgccg
    13621 gtcaggatcg acaggccggg accgaattcg aggtccagcc gatcgatcaa cacgatgtcg
    13681 cggatcgtca gccgaacgag catgggcgca acgccttcag gtcagcccgg cacgaccgtg
    13741 tgatagattt tcgaaatcca cgaatccttg ttctcttccg gctccagccc gccgcccttc
    13801 aacagagcgt aggcgtcctt gtaccattgg ctgtccggga aattgtggcc gagcaccgcg
    13861 gcggcggtct gcgcctcgct ggtgacgccg aggccgagat aggcttcggt caagcggtac
    13921 agcgcctctt cggcgtggcg cgtcgtctgg tagttcgcaa gcacgttgcg gaagcggttg
    13981 atcgccgcca cataattgcg ccggtgcaag tagaagcggc cgaccgacat ttccttgccg
    14041 gcgagttggt cgcgggcgac ttgaatcttg aacttcgaat cgttcacata ttccgagttc
    14101 ggatatttct gaacgatttg cgagaacaat tcgagcgcct tggcggtacg atcctggtcg
    14161 cgcgaaatat cgggaatctg cgcgtaatag gagctcgcct caagataggc ggcgtaggcg
    14221 gcgtccgacg attcgggata gagcttgacg tatttttccg ccgactgctc ggcattgtcg
    14281 tattcgccgg ccttgaattg cgaataggcc gtcatcagca aggccttttt cgaccactcc
    14341 gaatccggat aattcttgcc gagatcgacg aatttcttgg ccgcgcccga cccgtcctcc
    14401 ttggcgaggc gcgccaggcc ctggtcgtag atttgcgagg ccggcacgtc cggctcgact
    14461 ttcatcttgt atttttcggc gccgaacggg ttgtaatcgt tgatgtcgaa agacccgcaa
    14521 gcggcggccg gcgcggcgag gagaatggcc gcggcaagcc gcgcagcgcg ccccttggac
    14581 gtgatcggaa gaaaagcgaa atgcatgctg gagaactcga tcgtccctgc cccgagggcg
    14641 ccccttttag atcggccggc cgtcgccggc tgctttagac caaagcgcct ttcagcgcca
    14701 ccgatccttg cgcgaagcgg cgattaaccg ggaattgcgg cgcaatggcg ccttcgccgc
    14761 tcgcgatccg ccgtattcgc cgtattcgcc ggccgcggcg cggctgcggt ccggctcagt
    14821 cgacctccgc cgcataggcc gcgacaggga ggcggccgga caggacggca tgggccggcg
    14881 tcggcgcatg gcgcggcgct tcaatgaatt cgtaagccga gcggtcggcg aagagcgttt
    14941 gcagcgccgc caaattcatc gcatggccag ggcgataggc tttgaaggcg ccgaggatcg
    15001 gcgcgccggc cagggccaga tcgccgatcg aatcgagcat tttgtgccgc acgaactcgt
    15061 cgcggtaacg caggccctcg gggttgacga cccggtcgcc gtcgagagcg accgaattgt
    15121 cgagcgagga gccgagcgcg aaccccattt tccacaggcg ctcgacatcg ccgacaaagc
    15181 cgaacgtgcg cgcccgcgcg atctcgcggc ggaaagcgcc agcgtcgaga tcgaaaatct
    15241 tgcgctggcg gccgatcgcc ggcgtggcga agtcgatttc gacatcgagg cgaagcgcgc
    15301 gctcggcggg acgaagctcg gcataggcgt cgccgcgctc gacgcggacc ggcttgagga
    15361 ttttgagata gcggcgcggc gccgaaaagc tcgccaggcc gacctcgtcg atcgccgcga
    15421 caaaaggcgc ggccgagccg tcgaggatcg gcgcttcagg cccgtcgatc tcgatcgtca
    15481 cattgtcgac gccgagaccg gccaaagccg ccagcaaatg ctcgaccgtg gcgaccgagg
    15541 ccttggccgg atcgccgagc acggtgcaga gctgggtctg ggcgacgttc gaccatttgg
    15601 cttcgatcag cctttcgcgc ccgccgggca ggccggaacg caaaaaaacg acgcccgaat
    15661 tggcgtcggc tggatgaagt atgacggtga cgggttcgtt cgagtgtacg ccgacgccgg
    15721 aaatcgtcac gcgctcccgg agcgtcgtct gtcgaccaag tttcatcaaa ccccacatcc
    15781 cctcaaccaa accctcagac cctgttctta ggtctcgccc cggtccgctt gacgcggtct
    15841 caggtcgggt cggcgcttca cgcgggcggc ggctttggac gaatcgcccg ctttcgaatc
    15901 gggggaagga taaagggcgg ccagattgcg gccaaatcac tcttcgtgac cgtctgttac
    15961 aacgccgccg cgcctgacgc cgagcaaaac caattgttta ccggccgcgg cggcggagcc
    16021 ccgccgccgc ggccggacca agccttagct cgactggcgc cgcaggaaag ccggaatttc
    16081 gagctggtcc tcctcgaaag ccgtggtttt cggcgcgggc cggccatgcg gatcgagcgc
    16141 gccctgggcg acgcgcggcg ccaaggcgcg gggctgcggc cggccgtaat cggcatgcga
    16201 ttgcggcggc ggcgcggcgc ggacgggggg cgcgggcagg accggcgcgg cgcgcggcgc
    16261 gggcggcccg gtctcttcgt ggcgggtgat gccgaaagcg gcgagcttct ccagcagcga
    16321 tttgcggcgg ctttccgccg gcgccggctg ctcgccgcga tggatgcgca tctgctgctg
    16381 gacgaccggc ggcaaatctt cgatctgcgg catgcgggcc ggccggacga tctgctccgg
    16441 cgcgggcggc acgaattcgc tcgccggctc ctcgaacaag gtctgttgcg tcgccggctc
    16501 gacatagaag gcgggcttcg gcgcggctgg gcgaagctcg acctcggcgg cggccggcaa
    16561 gacgggcgcg gcgaccggcg ccatctcgac cggcgcttcg acgatcggct gaggcggggg
    16621 cggcgcagcc ggggcgggcg ccgcggcggc cgcgtggagg cgcgccgccg cctgcgcccg
    16681 caagcgctgc gcggcttcgg cgatgcgctg ctggctcgcc gacggctcct tggcctcgac
    16741 cttgtcctgg tcgatgccgg tcgcgacgac cgagacgcgc accacgccgt cgaggctctg
    16801 gtcgaacgtc gcgccgagaa tgatgttggc gtcctcgtcg acctcctggc ggatgcggct
    16861 cgccgcctcg tcgacctcgt aaaggccgag atcgttgccg ccggtgatcg agatgagcag
    16921 gcccctggcg ccgcccatcg agacctcgtc gagcagcgga ttggcgatcg ccgcctcggc
    16981 cgcctggatc gcgcggcgct cgcccgaggc ttcgccggtg cccatcatcg ccttgcccat
    17041 ctcgctcatg atcgagcgca cgtcggcgaa gtcgagattg atcaggcctt ccttgaccat
    17101 caggtcggtg atgcaggcga cgccggaata gaggacctgg tcggccatgg cgaaagcgtc
    17161 ggcgaaggtc gtcttctcat tggcgacgcg gaagagattc tggttgggaa tgatgatcag
    17221 cgtgtcgacg catttctgca gctcttcgat gccggcctcg gcgacccgca tgcgccgcgc
    17281 gccctcgaac tggaacggct tggtgacgac gccgaccgtc aggatgccca tttcgcgcgc
    17341 cacccgcgcg acgaccggcg cagcgccggt gccggtgccg ccgcccatgc cggcggtgac
    17401 gaagaccata tgcgcccccg ccagatgatc gcggatttcc tcgatcgcct cctcggcggc
    17461 ggcgcggccg acttccggct tcgagccggc gccgagccct tccgtcactt gcaggcccat
    17521 ctggatgatg cggtcggcct tggacgaagt cagcgcctgg gcgtcggtat tggcgacgat
    17581 gaagtcgacg ccgatcaggc cagacacgat catgttgttg acggcgttgc cgccggcgcc
    17641 cccgacgccg cacaccatga tgcgcggctt caattcccgg agctccgggg ctttcaaatt
    17701 gatggtcatc cttggcctct tgacgttaga cgcccgccgg cgccgggttg aattgcggtt
    17761 ggtcgattaa ggactcggtc gggaaggcgc gcggcgcctt ccggttaggc gtttaaaaac
    17821 tctccttcag ccagcggccg acgcgcgcga catagccgtc gcccgcggcg ctcgatgaat
    17881 aggatcgccg gccaggctcg aaatgctcga ttccggcgac ttgcgggtag accatcaggc
    17941 cgaccgcggc ggcgaaagcc gggctcttcg ccgattccgg caggccgtcg acgccgagcg
    18001 gacggccgat gcggatctgg ctggagataa tccggcgcgc cagctcgggc aggccggtga
    18061 gctggcaggc gccgccggtc aggaccagcc ggcgcccggc atgggccgcg tggcccgctt
    18121 gcttcaggcg gtcgcggacc agttcgagaa tctcctcgac gcgcggccgg atgatgcgca
    18181 ccagctcgga tttcggcagc acgctcggat ggtcgaggtc gtcgccgacc cgcgccaccg
    18241 tcatcgtgtc gcgctcgtcc gacggcgagg cgatgcagct tccatagagg gtcttcaggc
    18301 gctcggcgtc ggcgagcgac acggcgaggc cgcgggcgat gtcggtggtg atgtgattgc
    18361 cgccgaccgc caccgcgtcg acatgggcga ggcggccgcc gacaaagacg ccgatcgagg
    18421 tcgagccgcc gccgaaatca atcaaggccg agccgagctc ggcttcgtcg tcgaccaggg
    18481 tcgaaagccc ggccgcatag ggcgtcgcca ccaccgcgtc gacgcgtaaa tgacaacgct
    18541 cgacggcgag catcaggttg cgcgccgccg cgccgtcgca ggaggcgaca tgaagctcgg
    18601 cgccaagctc gtgcgccacc atgcctttcg ggtcgcgcac gccgcgcgtc gcatcgaggg
    18661 aaaagcccat cggcaaggcg tgcagcgcga tccgccccgc cggcgcgctg gcgtgcgagg
    18721 ccgcctcgag cacgcgatga atgtcgtgct cgctcaccgc cttgtgggcg atggcgacgc
    18781 gcgcattgta gaactgcgag gcgaggcgtc cgccggtcat gttgacgatg atgctttcga
    18841 cctcgacccc ggccatgcgc tcggcggcgt cgatggcgag ccgcatcgcg gtctcggccg
    18901 cgtcgagatc gacgatcgcg ccggccttga cgccgcgcga gcgctgatgg ccgatgccga
    18961 gcaccttgca gcgatgggtg cggccgcgca gcgaatcgga ggccggcatc ggcgtcagcc
    19021 tcgcgatcag gcagacgatt ttcgcagcgc cgacatcgag caccgacagg acggcgctct
    19081 tgcgcggcgg gatttgccga aggcgggggg tcaccggctt gaagctcatg tcgccccctt
    19141 cttcgccgga cgcgcggcgg cggcgcgggc ggccgccgcg tcttcggtca agcggacgaa
    19201 cattttgccg gggacgcgaa agtcgagcga cagagccgcg cgttcgagca cgcggtcggt
    19261 gcgctgcagg cggaccagcg tcgccatcgc cgcctcagga tcggcctcgg gcagcttgac
    19321 gaccatgccg ttggtcatcg ccagatccca gcggcggccg gagacgagga cgcctgcctt
    19381 gatcttcgga cgcaattcgc ccgccgcgtc gaggatcgac atgaattccg gcagcctctc
    19441 attggcgccg tcgccgacga cgaaaggcag gccgatgaag cggtcgtcgg tcatccggtc
    19501 gatcggcgcg ccgtcggcgg cgacgatcgc gaccttgccg tccttctgcc acagcgcgta
    19561 aggcgcgcgt tcgacgagat cgatgacgag atggttggga tagagcttgc gcacgctcgc
    19621 cgatttgacg agcggcaagg cttcgaggtc cttgcgcgcc gcggcggcgt cgaggaacag
    19681 cagcgaatct ttcggcgtga tatgggcggc ggcgaggatt tcccgctcgc gcagttcgac
    19741 ctggccggaa atggtgacga tggcgatgtc gaagccgagc gcccgcgcga tcacgtcgcg
    19801 cagatcgccc tctctggcga tgaaggcttg atactcgccg ccgcgcaccg cgccgaacgc
    19861 cgcgacgccg cccaagaggg cgacggccaa agcgaggccg agaccgcgcg gcgccgagag
    19921 cagaaagcgg cgcagccgcg tcaggccgcg gccggcgctg cgctcgccgc gcagcaggac
    19981 gagcggcgcg cggatttcgc cggcgccgat cgcagccgga gccagaggcg ccgaaaggcc
    20041 gccgagcgcg aaagcttcgg ctatcggtcg catgacgcgt cctcgaccat ccaggaaacg
    20101 agttcggcga aggaaatgcc ggcataggcc gccatttccg gcaccaggga agtttcggtc
    20161 atgccgggct gcgtgttgac ctccaagcag accagctcgc ccttctcccc cttcgcctcg
    20221 tcgtagcgaa aatccgcccg gctcacgccg cggcagccga gcgcccgatg cgccctgagg
    20281 ctcaactgtt gaatgtcatg gtaaatattc ggtttaattt tcgccggaag gacatggatc
    20341 gagccgcctt tggcgtattt cgcgtcatag ccgtagaaag cttcggagac cggttgtact
    20401 tcgatcacgt cgaagaccct gtcgcccatc accgcgcagg tgagctccag cccggcgaca
    20461 tagcgctcgg cgaggaggac gtcgccatag ggccagtcgg gacggccgac ttcctgcggc
    20521 gggtgcgagc ggccctgctt gacgatgacg acgcccagcg aggagccttc gtagagcggc
    20581 ttcaacacgt aaggcggctc cagcgcgtgg cgcttggccg cgtcgtggcg gtcgacgacc
    20641 aggccatggg cgacgggaat tcccgccgcc gccatcaccg tcttggcgag gtccttctgc
    20701 aaggcgaggg ccgaagcgag cacgccggaa tgggtgtagg ggatgctcag gatttcgagc
    20761 acgccctgga tcgtcccgtc ctcgccgaac cggccatgga gggcgttgaa ggcgacgtca
    20821 ggcttgagct tggcgagcac ctcggcgatg tcgcgctgca cgtccacccg ggtgacgcga
    20881 tagcccgcgc tttcgagggc ggcggcgcag ccggccccgg agcggagcga gacttcccgc
    20941 tcccccgacc agccgcccat cagcaccgca acatgtttca tcgacgtaaa tgcgccccaa
    21001 ccggctcaag ccgtgattaa gcgaaaatta aaggcagggc gttaaggacg cgttaaccag
    21061 gggtttgcgg cgaggatatg ggcacgattt aacgttggcg accgccgcgc cattcgctcg
    21121 ccttcgataa tgaagcgcgg cgccgaagcc ccctctcccc gtggagcgaa gcggaacggg
    21181 g
//
LOCUS       DATGJW010000458         4776 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1127966, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000458 DATGJW010000000
VERSION     DATGJW010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4776)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4776)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4776
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1127966"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VKU03_13820"
     CDS             complement(<1..239)
                     /locus_tag="VKU03_13820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SsrA-binding protein"
                     /protein_id="HLJ72393.1"
                     /translation="MSGKDKPTYKIVADNRRARFDYEIGETFEAGIALTGTEVKSLRA
                     GKANVAESYAAADRNGEMVLYNANIPEYTQGNRFN"
     gene            complement(461..1354)
                     /gene="dapA"
                     /locus_tag="VKU03_13825"
     CDS             complement(461..1354)
                     /gene="dapA"
                     /locus_tag="VKU03_13825"
                     /EC_number="4.3.3.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008840 - 4-hydroxy-tetrahydrodipicolinate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate synthase"
                     /protein_id="HLJ72394.1"
                     /translation="MVEKAKFHGSMPALVTPFKDGKLDEQAFRGLIDWQISSGSHGLV
                     PVGTTGESPTLSHEEHRRAVSICIDEARGRVPVIAGAGSNNTHEAIELARHAEKAGAD
                     AVLVVTPYYNKPNQEGLYRHFKAVNDAVGVPIIIYNIPPRSVIDMSVETMKRLYELKN
                     IVGVKDATSNIGRVSLQRQAMGPGFIQLSGEDMTALACMAAGAHGCISVTANVAPALC
                     AELMEATFKGDYAHALRVQDRLTALHNALFIEPGVCGAKYALSLLGRVRNEMRLPLVP
                     IVEATEAAIRRAMVHAGILNA"
     gene            1536..3815
                     /locus_tag="VKU03_13830"
     CDS             1536..3815
                     /locus_tag="VKU03_13830"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLJ72395.1"
                     /translation="MAGAFLPTSVFFAVAIIAGGPPHAPLPLPSPSFGALVPLPKSAP
                     PAPNPTPAGAAPASDSQAPALAGGALQRAGSTIVPIAPDGFQDLIQLADLGARQLDMG
                     SNDVASAGRRGFAWLLTKTPLGGADFSILTAAAALYQKGDAAGGDALAARLDDPLQRL
                     ALEWVALRAAPREAGLTRLTSFMDAHPHWPSRGWLVSFREALLYADRNRPDVIGKAFA
                     GAPPATPAGILALATVERDGGHADKAAAMVRDLWRDGDLDGWLEGAILKDFGGLLTKA
                     DHRARAIRLLYAEKYGAAARAAALAGGDTPLLANSWVAASRGALSDKALAALPAAVKN
                     APGLLYARIQGLRRADRALEAAQLMRAAPKDPAALVDGDRWWAERRMIARRLLDGALA
                     KDAYQLCAEPAAVSVAARVDQDFHAGWIALRFLNDPADAARHFAALASLAQTPLTAAR
                     AAYWQGRAAEQAAQPEQAKLFYERAAAFSSAYYGQLSAERLDRPLAFHAAVHAAKADA
                     RDEATRVVELLYDAKLNVFARVLAIDAAKTYTDESQLAALARVVQRVADAPTAVEIGK
                     QAMLRGFSLDEAAFPTYGVPQFSPLDGSADLPLVYSVARQESEFATAAASGAGAKGVM
                     QLLPQTARDTARRAGIAFDLTRLITDPSFNVQLGAAFLGQLLADEGGSIVLALAAYNA
                     GGGRVQQWIQRYGDPRQPGVDPVDWVERIPFDETRDYVQRVMENWRVYRARFSEAAAA
                     SAPPPRSAELPADERFDVN"
     gene            complement(4087..>4776)
                     /locus_tag="VKU03_13835"
     CDS             complement(4087..>4776)
                     /locus_tag="VKU03_13835"
                     /inference="COORDINATES: protein motif:HMM:NF018527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HLJ72396.1"
                     /translation="AHSLALVYVLDAVTPWSMTMMKALSTSTCLAAVSFCAALAISQM
                     AVAQDNGASQKGHEQSGSQPTNKEVQTETSKPKSAPSAERHGAEKMQTGETGVKEKAK
                     PEMKKGDAAEQQRSEKRSGEAAQEKNGGAKMTTGRAAAESGHTMRLDQRQSADFRSRL
                     GQRGEAASLPSGFSVRTGVALPETVRLLELPPEIFVEYPQFRGYDYIVFRNEIIIIDP
                     RTREIVDVISG"
BASE COUNT          763 a         1641 c         1541 g          831 t
ORIGIN      
        1 tggttgaagc ggttgccttg cgtatattcc gggatgttgg cgttgtagag gaccatctcg
       61 ccgttgcggt cggcggcagc gtagctctcg gcgacattgg ccttgccggc gcgcagggat
      121 ttgacctccg tgccggtcaa ggcgatgccc gcctcgaacg tctcgccgat ttcgtaatcg
      181 aaccgggcgc ggcgattgtc ggcgacgatc ttataagtcg gcttatcttt gcccgacacg
      241 gatccagcct ctaagcgggt tgtgacttaa aaaagtacgg atggtcgcgc gacttaccct
      301 catccggcgc ttcgcgccac cttctcccgc aagcgggaga agggcgacag cggctacagc
      361 gcggcggcta atgaagtccg cagcgcgccc cctctcccgc ttgcgggaga gggttgaggt
      421 gagggcaaga agcgacatag gtcggcaaag tgaaatccga tcacgcgttc aaaatcccgg
      481 cgtgaaccat ggcgcggcgg atcgccgcct cggtcgcctc gacgatcggg acgagcggca
      541 ggcgcatttc gttgcgcacc cggccgagca ggctcagcgc gtatttggcg ccgcagacgc
      601 cgggctcgat gaagagcgcg ttgtgaagcg cggtcaggcg gtcctggacg cgcaaagcgt
      661 gcgcgtaatc gcccttgaac gtcgcctcca tcaattcggc gcagagcgcc ggcgcgacat
      721 tagcggtgac cgagatgcag ccatgcgcgc ccgccgccat gcaggcgagc gcggtcatgt
      781 cctcgccgga aagctggatg aagccgggcc ccatcgcctg gcgctgcaat gagacgcggc
      841 cgatattgct ggtcgcgtcc ttcacgccga caatgttctt gagctcgtaa agccgcttca
      901 tcgtctcgac cgacatgtcg atcacagagc gcggcgggat attgtaaatg atgatcggaa
      961 ccccgaccgc gtcgttcact gccttaaaat ggcgataaag tccctcttga ttgggcttgt
     1021 tataataagg ggtgacgacg agaacggcgt cggcgccggc cttttcagca tggcgcgcaa
     1081 gttcgatggc ttcatgcgtg ttgtttgatc ccgccccggc gatgaccggc acgcggccac
     1141 gcgcctcgtc gatgcagatg ctgacggcgc ggcggtgctc ctcgtggctc aaggtcgggc
     1201 tttcgccggt cgtgccgacc ggcacaaggc catgggagcc ggaggaaatc tgccagtcga
     1261 tcaggccgcg aaaggcttgc tcgtcgagct tgccgtcctt gaacggcgtc acgagagccg
     1321 gcatcgagcc gtgaaacttc gccttttcaa ccatggggcc ccatcaaagc cgaccgatcg
     1381 ccatcaaaag caatggcctc gcgctcaatc ggagcggcga gtcataaacc gtcggtcggc
     1441 aaggggaaag ggggacgagg gaccgtaaga aaacgccgat gtggttagcg attcgtaagc
     1501 aaaaggagcg caagctgaag ccgtgggggc ttcgcgtggc tggcgcattc cttccgacgt
     1561 ccgtcttctt cgccgtggcg atcatcgccg gcggccctcc gcatgcgccc ctgcccctgc
     1621 cgtcgccgag tttcggcgcg ctcgttccgc tgccgaaaag cgcgccgccg gcgccgaacc
     1681 cgacgccggc cggcgcggcg ccggcttcgg acagccaagc gccggcgctg gcgggcggcg
     1741 ctcttcaacg ggccggttcg acgatcgttc cgatcgcgcc cgacggcttc caggatctca
     1801 ttcaactcgc cgatctcggc gcgcggcaat tggacatggg gtcgaacgac gtcgcctcgg
     1861 cagggcgccg cggctttgcc tggctgttga cgaaaacccc gctcggcggc gccgatttct
     1921 ccatcctgac cgccgccgcc gcgctctacc agaagggcga cgccgccggc ggcgacgcgc
     1981 tcgccgcccg cctcgacgac ccgctgcagc gcctggcgct ggaatgggta gccttgcgcg
     2041 ccgcgccgcg cgaagcgggc ctgacgcggc tgaccagctt catggacgcc catccgcatt
     2101 ggccgtcgcg cggctggctc gtctcgttcc gcgaggccct gctctacgcc gatcgcaacc
     2161 ggcccgacgt catcggcaag gctttcgccg gcgcgccgcc ggccacgccg gcgggaatcc
     2221 tcgccctcgc gaccgtcgag cgcgacgggg gccatgccga caaggccgcc gcaatggtgc
     2281 gcgatctttg gcgcgacggc gatctcgacg gttggctcga aggcgccatc ctgaaggatt
     2341 tcggcggcct cctcaccaag gccgaccatc gcgcccgcgc cattcgcctt ctctatgccg
     2401 agaaatacgg cgccgccgcc cgcgccgcgg ctctcgccgg cggcgatacg ccgctcctcg
     2461 ccaattcgtg ggtcgccgcg tcgcgcggcg cgctttccga taaggcgctc gcggctctgc
     2521 cggcggccgt gaagaacgcg cccggcctgc tctacgcccg catccaaggg ctgcgccgcg
     2581 ccgaccgggc gctggaggcg gcgcaactca tgcgcgccgc gccgaaggac ccggccgccc
     2641 tcgtcgacgg cgaccgctgg tgggccgagc ggcggatgat cgcgagacgg ctgctcgacg
     2701 gcgcgctggc caaggacgcc tatcagctct gcgccgagcc ggcggccgtc tcggtcgcgg
     2761 cgcgcgtcga ccaggacttc catgccggct ggatcgccct gcgcttcctc aacgatccgg
     2821 ccgacgcggc gcgccatttc gccgcgctgg cgagcctcgc ccagacgccg ctcaccgcgg
     2881 cgcgggccgc ctattggcag ggccgcgccg ccgagcaggc ggctcagccc gagcaggcca
     2941 agcttttcta cgagcgcgcc gccgcctttt cctcggccta ttacggccag ctctcagccg
     3001 agcggctcga ccggcccctc gccttccacg ccgcggtcca cgccgccaaa gccgatgcgc
     3061 gcgacgaggc gacgcgcgtc gtcgagttgc tctacgacgc caagctcaac gtcttcgcgc
     3121 gcgtcttggc gatcgacgcg gccaagacct acacggacga atcgcagctc gccgccctcg
     3181 cccgcgtcgt ccagcgcgtc gccgacgcgc cgaccgccgt tgagatcggc aagcaggcga
     3241 tgctgcgcgg cttctcgctc gacgaggcgg ccttcccgac ctatggcgtg ccgcaatttt
     3301 cgccgctcga cggctcggcc gacctgccgc tcgtctattc ggtggcgcgc caggaaagcg
     3361 agttcgccac cgccgccgcc tccggcgccg gcgccaaggg cgtcatgcaa ttgctgccgc
     3421 aaaccgcgcg cgacacggcg cggcgcgccg gcattgcttt cgacctcacc cgcctgatca
     3481 ccgatccgag cttcaacgtg cagctcggcg cggcgtttct cggccagttg ctcgccgacg
     3541 aaggcggctc gatcgtcctc gccctcgccg cctacaatgc cggcggcggc cgcgtgcagc
     3601 aatggatcca gcgctacggc gatccgcgcc agccgggcgt cgatccggtc gattgggtcg
     3661 agcgcatccc gttcgacgag acgcgcgact acgtgcagcg ggtgatggag aattggcggg
     3721 tgtaccgcgc gcgcttcagc gaggccgccg ctgcgtccgc gccgccgccg cgcagcgccg
     3781 agcttcctgc ggacgagcgt tttgacgtaa actgaaagaa aattgcgcca atatcattga
     3841 agccgctgta accctatagg gaaagtaccc cgccaacgta tcggcgccgc cgcccatctc
     3901 atgcgcaacg cgaggggcga gattgagggt tagcgagcaa gcgggatcgg gcgggccttt
     3961 gacgaacgcg accgtagaaa tttgagccca tcgccgcgtc atcgcgattc ttgaaaggct
     4021 catcagtgaa acgccccctc ggccggtacc tcgccgagac gaacgctcag cgaggcagac
     4081 gggtgattat ccgctgatca catcgacgat ttcgcgcgtg cgcggatcga taatgatgat
     4141 ttcgtttctg aagacgatgt agtcatagcc gcggaactgc ggatactcca caaagatctc
     4201 tggcggaagc tccagaagcc ggaccgtctc agggagcgct actcctgtgc ggactgagaa
     4261 gccggagggg agggatgcgg cctcgccccg ctgtcccaac cggctacgga agtcagccga
     4321 ctggcgttga tcgagcctca tcgtatggcc agattcggcc gccgcccggc ctgtcgtcat
     4381 cttggcgccg ccattcttct cttgcgcagc ttcgccggac cgtttctcag atcgctgctg
     4441 ttcagccgcg tcgccctttt tcatttccgg cttcgccttc tctttgacgc ccgtctcgcc
     4501 ggtctgcatt ttttctgcgc catgcctttc agcagaaggc gcgctcttgg gcttgctcgt
     4561 ctccgtttgc acttccttgt tcgtaggctg cgaacccgat tgttcatgtc ctttctggct
     4621 cgcgccgttg tcttgagcga cagccatttg gctgatcgca agcgccgcgc aaaacgaaac
     4681 cgccgctagg caggttgatg tcgatagagc cttcatcatg gtcatactcc aaggggtgac
     4741 cgcgtccaga acgtaaacaa gcgcgaggct gtgcgc
//
LOCUS       DATGJW010000459         3732 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1334613, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000459 DATGJW010000000
VERSION     DATGJW010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3732)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3732)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3732
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1334613"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1785)
                     /locus_tag="VKU03_13840"
     CDS             complement(<1..1785)
                     /locus_tag="VKU03_13840"
                     /inference="COORDINATES: protein motif:HMM:NF012862.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="caspase family protein"
                     /protein_id="HLJ72397.1"
                     /translation="MTFERKVFRMLWGAALALAINGAAFAQQPPNPRIALVIGEAAYP
                     DNPLATTANDAGLVAQTLQAAGFDVVGARDLDEKSLRQAFRDFIDKANAAGPDGIDFV
                     YLSGRALQYAGDNYFAPVDARIARDVDVPIEAVKLGDFTHALAAISGRSHIIVIDGAR
                     ANNYAPETPLAPGLALVDAEKGELIAYNAAPGTLAPDETGAYGVYGKDLAGALRQGGV
                     PLEDIFAQVRLQVNQDTQGAVVPWSAANLAGPVYIFERAADTQPPPVIAATKALATRP
                     LRSLGAREAYAAAIERDTLEAYREYLEAYPHAAEARRIRAMLAARREASYWRRALREN
                     TPRAYWTYLRRYPNGPHVADASRRLAFLSAPEAPPSDFEPVEFADLPPPPPDEDFYEE
                     RPVAYFGGDDFGPPPPPPPPDFTPDYGEDWRDLPPPPPPREYGYLPVLPIAIPLIAGA
                     VAIDYLHHERHERRRAEERNGVAPPGQPSAPRFGQAGQPPAPKPAAPPPLPKGVTVKA
                     PPPQQQNAPKPLIPGTPGARPVSTAPAAIKATPPQPAPSQPAPAGGHAAPQPQPQAPA
                     GHALPQPVAPQPTSGGHAAPPQPQAAAGR"
     gene            complement(1943..2296)
                     /locus_tag="VKU03_13845"
     CDS             complement(1943..2296)
                     /locus_tag="VKU03_13845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72398.1"
                     /translation="MTARKMMGAAVVVLPLISFLFLLIVNPAAADWFARTLLARLTDP
                     IIVLETILSAAAGAARRGLIWPLALAMATGLRFAISYSMWGAGAGDAFAEHAVHAAEY
                     ALAFAFCGYLLGRAC"
     gene            complement(3577..3732)
                     /locus_tag="VKU03_13850"
                     /pseudo
     CDS             complement(3577..3732)
                     /locus_tag="VKU03_13850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333165.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="IS481 family transposase"
BASE COUNT          649 a         1174 c         1302 g          607 t
ORIGIN      
        1 gcggcctgcc gcagcttgcg gttgcggcgg cgcggcgtgg ccgcctgaag tcggttgcgg
       61 cgcgacaggc tgcggcaatg cgtggccagc gggcgcttga ggctggggct gcggcgcggc
      121 gtggccgccg gccggcgctg gttgggaagg cgcgggctgc ggcggggtcg ccttgatcgc
      181 ggccggcgcc gtcgagaccg ggcgggcgcc gggcgttccg ggaatgagcg gcttcggcgc
      241 gttctgttgt tgcggcggcg gcgctttcac cgtcacgccc ttgggcaaag gcggcggagc
      301 cgcaggtttc ggcgcaggcg gttgcccggc ttggccaaag cgaggcgcgc tcggctgtcc
      361 gggcggggcg acgccgttgc gttcttcggc ccggcggcgc tcatggcgct cgtgatggag
      421 atagtcgatc gccaccgcgc cggcgatcag cgggatggcg atcggcagga ccggaaggta
      481 gccatattcg cgcggcggcg gcggcggcgg caggtcgcgc caatcctcgc cgtagtccgg
      541 cgtgaaatcc ggcggcggcg gcggcggcgg cgggccaaaa tcgtcgccgc cgaaataggc
      601 gaccggccgc tcctcgtaga agtcctcgtc gggcggcggc ggcggcagat cggcgaactc
      661 gaccggctcg aaatcggacg gcggggcctc gggcgcggag aggaaggcga ggcgccgcga
      721 ggcgtcggcg acatgcggtc cgttcggata gcgcctcaga taggtccaat aggcgcgcgg
      781 cgtattctcg cgcaaggcgc gtcgccaata cgacgcctcg cggcgcgccg cgagcatcgc
      841 gcgaatgcga cgcgcctcgg ccgcatgcgg ataggcttcg agatattctc gataggcttc
      901 gagcgtgtcg cgctcgatcg cggcggcata ggcctctctc gcgccgagcg atctcagcgg
      961 gcgggtggct aaagccttgg ttgccgcgat caccggcggc ggctgcgtat cggcggcgcg
     1021 ctcgaaaata tagacgggcc cggcaagatt ggcggcgctc caaggtacga ccgcgccctg
     1081 cgtgtcctgg ttcacctgca ggcgcacttg cgcgaaaatg tcttcgagcg gcacgccgcc
     1141 ctggcgcaac gcgccggcga gatccttgcc gtagacgcca taggcgccgg tctcgtccgg
     1201 cgccagcgtg ccaggcgcgg cgttataggc gatgagctcg cccttctcgg cgtcgaccag
     1261 cgcgaggccc ggcgcaagcg gcgtctccgg cgcataatta ttggcgcgcg cgccatcaat
     1321 gacaatgata tgcgagcggc cggatatggc ggcgagcgca tgcgtgaaat cgccaagctt
     1381 cacggcttcg atcggcacat cgacatcccg cgcgatccgc gcgtcgacgg gcgcgaagta
     1441 attgtcgccg gcatattgca gcgcgcggcc ggatagatag acgaaatcga ttccgtcggg
     1501 gccggccgca ttggccttgt cgatgaagtc tcggaaggcc tggcgcagcg atttctcgtc
     1561 gaggtcgcgc gcgccgacca cgtcgaagcc cgccgcctgc aaggtctggg cgaccagccc
     1621 ggcgtcgttc gccgtcgtcg ccaagggatt gtcgggatag gcggcctcgc cgatcaccag
     1681 cgcgatgcgc ggattgggcg gctgctgcgc gaaggccgcg ccgtttatcg ccaacgccaa
     1741 agcggcgccc caaagcatgc ggaagacttt tctttcgaac gtcatgttgt cctggagcgc
     1801 tgaaggagat tcaggtgggc cgcgcaatct gcgccgccgc gccttgtttg acaagcgagg
     1861 ctgcaggcga cgcgagcgca cgggcgagaa cttggcgaaa atatggacct cgctccgcgc
     1921 gacgaggcgc gacaatgcgc cactagcagg ctcggcctaa aagatagccg caaaaggcga
     1981 aggctaacgc atattcggcg gcatggacgg catgctcggc gaatgcatcg ccggctcctg
     2041 cgccccacat ggaatagctg attgcaaacc tcaggccagt ggccatcgcc aacgccaacg
     2101 gccagatcaa gccacgacgc gccgcgcctg cggccgccga caagattgtc tccagcacga
     2161 tgatggggtc ggtcaatctt gcgagcagcg tccgggcaaa ccaatcggcc gcggccggat
     2221 tcacgatgag caagaacaga aaggaaatga gcggcagcac gacgaccgcc gcgcccatca
     2281 tctttctcgc cgtcatctct cgcgctccgt gacctgatgg ctttgcggct gaggctccac
     2341 tcagcgggtt cacgaccact gccgagaatg gctccattca aggagcttca acaggaaaca
     2401 gcaatcgacg agaagaaaaa ggtctggcgc gctacacagc gagcggagcg tacatcccgt
     2461 cgcgatgcag ccttgacgga tttcccgaga ggtcgtgcta ggcgacgacg ccttccgcga
     2521 aatagtggca gaccagcgcc ctccgcgata ggttgggatc gttgcgcggc gagccgccat
     2581 gcaccagatt ggcgcgccag aaaaagaaca tccccctttt tggcgaggaa gatctgtttc
     2641 gtcaggcctg cttcctcgca gagcctctgg accatcggct cgtatttctc gctacagatc
     2701 gcgtagcctt ttttccttga atgcctgcgg gcgggccgct cgcagggtga aatgtcttcg
     2761 aaggcgatcc aattgcgctt ggccgcatcg ccgcgccccg gcgcgacttt cgcgttgcgc
     2821 cctccggcct cagcgaaagg ttctctccaa gcgctttcaa ccccgcggac cgtcagcacc
     2881 gtccgagacg cgttcgtaac gggggctggt cacggatacg gccggaatat cggccggaaa
     2941 tattgcgttg cgacgcaatt cttggcctct tcaccccctg gactaaggcg ggttcatgag
     3001 atacaactct ttgtttttat tatctaaaac gcatagaggg ccgccgccgc aagaggtcac
     3061 ggggcaaccg tcggtcgcta ggaaatccca gggcggcatg gtcagcggac agcaatgatg
     3121 gcttttcttc tccaaagcca tcaggcggcg gacgagaatc gccgcctcgg cgtcggtgag
     3181 cggcttgacg cgacgcatct cgggctgcgc ccgaaaggcg gaggccagaa cgaccggcga
     3241 tgggagcgtt ttccagtgcg tgatcgcgcg ctcttggcaa accgccgaga agggatggcg
     3301 ccaggggcgc ggctcgtccg cagacgcgac gaacagccaa tcggcgtccg tcgtcgcctc
     3361 gctaacgcgg ccggaatggc gatcgtcgag ttcgcgcaga aattccgcgg cggtgtctcg
     3421 cgattcttcg ccccaacggc aaaagacgct ggccgagcac agttcgacgc cggcgaacaa
     3481 aaccggcgcg cccggcccgg cttgccgctg cgcgctgatc cagaagcggc cagggacatg
     3541 gcgagcgcgg gtcttggcga gggtggtggt cagcccggcg cggaggcggc caattccgtg
     3601 gcgctgaaag cagcgatgca gcgccgaacg ggtgagtttc ggcaggactt cgcgcagttt
     3661 ctcgaggcaa tcgtcgaggg tgagccaggt catcaggcgg tattggacga caaaggcctc
     3721 ctcggccgga tt
//
LOCUS       DATGJW010000460         9949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1537744, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000460 DATGJW010000000
VERSION     DATGJW010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9949
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1537744"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            339..749
                     /gene="sdhC"
                     /locus_tag="VKU03_13855"
     CDS             339..749
                     /gene="sdhC"
                     /locus_tag="VKU03_13855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174253.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, cytochrome b556
                     subunit"
                     /protein_id="HLJ72399.1"
                     /translation="MASTDVSPSPARARPLSPHLTVYRWTMTMAMSIFHRITGGALYV
                     GTILLAWYLIALSMDESAFGVVSGFLNSIIGQIILFGFTWALIHHALGGIRHAIWDTG
                     YGMEKPMRDQLACATLIGGVVLTIVVWIIGYAVR"
     gene            759..1148
                     /gene="sdhD"
                     /locus_tag="VKU03_13860"
     CDS             759..1148
                     /gene="sdhD"
                     /locus_tag="VKU03_13860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0045282 - plasma membrane succinate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0000104 - succinate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase, hydrophobic membrane
                     anchor protein"
                     /protein_id="HLJ72400.1"
                     /translation="MTSDDNSLRSPLARVRYLGSARSGARENWLMRVTSAALIPLVIA
                     FVWVMLSLLSKDYNGARAELGRPLPAIIVMLFVLVGIYHMQIGMRSIIFDYVHGRAKE
                     WALIGNLFFAGALALACVYSVLRIGFV"
     gene            1159..2994
                     /gene="sdhA"
                     /locus_tag="VKU03_13865"
     CDS             1159..2994
                     /gene="sdhA"
                     /locus_tag="VKU03_13865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004446645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006099 - tricarboxylic acid cycle
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase flavoprotein subunit"
                     /protein_id="HLJ72401.1"
                     /translation="MATNGKNGASAPAQNGRAYPITDHTFDVVVVGAGGAGLRATVGC
                     AQGGLRTACVTKVFPTRSHTVAAQGGVAASLGNMGLDDWRWHMYDTVKGSDWLGDQDA
                     IEYLCRHAPAAVYELDHWGVPFSRTEDGRIYQRPFGGMTTDYGKGIAQRTCAAADRTG
                     HAILHTLYGQALKAKAEFFIEFFAIDLIRDSDGRVRGVVCLKLDDGTIHRFRAHLTVL
                     ATGGYGRAYFSATSAHTCTGDGNAMALRVGLPLQDMEFVQFHPTGIYGAGCLITEGSR
                     GEGGYLTNSEGERFMERYAPSAKDLASRDVVSRAMTMEIREGRGVGKLKDHIYLHLDH
                     LDPKILHERLPGISESARIFAGVDVTKEPIPVLPTVHYNMGGVPTNYHGEVVTKANGD
                     PDKIVPGLMALGEAACVSVHGANRLGSNSLIDLVVFGRAAGLRARELVEPGESQPELP
                     KDSADFALSRLDRFRHANGGTPTAELRGRMQRTMQANCAVFRTGEVLHEGQKLIHDVW
                     RAGADLKVTDRSLIWNSDLVETMEFDNLIAQAVVTMDSAANRLESRGAHAREDYPNRD
                     DKEWMKHTLAWIDPEKQTVTIDYRPVHTYTMTNDVTYIEPKARVY"
     gene            3004..3249
                     /locus_tag="VKU03_13870"
     CDS             3004..3249
                     /locus_tag="VKU03_13870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920915.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72402.1"
                     /translation="MNALTIEKLSPQTWSNLSARARAHHNSIEDEAAAILESALNEGP
                     GKSERQRIVDRIAAMTPKGVVQDDSTLFIRAERDRQR"
     gene            3246..3692
                     /locus_tag="VKU03_13875"
     CDS             3246..3692
                     /locus_tag="VKU03_13875"
                     /inference="COORDINATES: protein motif:HMM:NF013970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ72403.1"
                     /translation="MNLIIDASVAVKWFVEEERRDLACDVLRDGFNLFAPDLALVEVA
                     NALRNKVRLGRMNYEQARASLTVFPGLFSRLVPQIETFREAFEIGCAINHSAYDCVYL
                     ACVKILNTALLTDDATLFQKASALDLGAKTLRLSDWKPGLPANTTV"
     gene            3697..4485
                     /locus_tag="VKU03_13880"
     CDS             3697..4485
                     /locus_tag="VKU03_13880"
                     /EC_number="1.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006467970.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008177 - succinate dehydrogenase
                     (ubiquinone) activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="succinate dehydrogenase iron-sulfur subunit"
                     /protein_id="HLJ72404.1"
                     /translation="MVQFNLPKNSRMTGGKTWPKPAGAKRTTEFRIYRWNPDDGANPR
                     IDTYTVDRDDCGPMVLDALIWIKSKIDSTLTFRRSCREGICGSCAMNIDGGNTLACTR
                     GMDEESKGGGPIKIYPLPHLAVVKDLVPDLSNFYAQHASIEPWLKTVTPAPEKERRQS
                     PADRAKLDGLYECILCACCSTSCPSYWWTGERYLGPAVLLQAYRWLIDSRDEATGDRL
                     DNLEDPFRLYRCHTIMNCAQTCPKGLNPAKAIAEIKNLMIERQM"
     gene            complement(4536..4832)
                     /locus_tag="VKU03_13885"
     CDS             complement(4536..4832)
                     /locus_tag="VKU03_13885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009762742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ72405.1"
                     /translation="MTKLGQHLIRAAKEARAIAKGEADPASYRVHVPDRIDVKAIRAK
                     LGLSQNAFAARFGISPGTLKNWEQGLRDPDAQARVLLTIIDREPEAVQRALRQM"
     gene            complement(4829..5197)
                     /locus_tag="VKU03_13890"
     CDS             complement(4829..5197)
                     /locus_tag="VKU03_13890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015822169.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HLJ72406.1"
                     /translation="MHPVIETPSYLGDLNSAGVTIEGAARIVAIIASDPEAGDLIVGT
                     GGARKVRIGGRGKGKSGGYRVVTYYAAKEVPAFALISKGERADLSQAERNALKAELAS
                     LADDYRAGLAAKVNKLRTSP"
     gene            5341..6030
                     /gene="tsaB"
                     /locus_tag="VKU03_13895"
     CDS             5341..6030
                     /gene="tsaB"
                     /locus_tag="VKU03_13895"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007566628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HLJ72407.1"
                     /translation="MRILAIDTSCGAASACVAESGAAEPVAVETLPMQRGHAEALAPM
                     LQRLMAGVEGGFASLDRIAATVGPGSFTGIRIGLATARAIGLGLSLPVVGVSSLVAFA
                     GPLLADPKPGVIAASIDAKHGKVYLQLLEANGRPLLPPRLAALRDAIRLIGAGPVRLT
                     GNGAALLAAEAARIGLAAEIAGNLDYPDIVAVARLGLVAEPSQSPPRPLYLKAADAQA
                     PENPAVARAGS"
     gene            6053..6532
                     /gene="rimI"
                     /locus_tag="VKU03_13900"
     CDS             6053..6532
                     /gene="rimI"
                     /locus_tag="VKU03_13900"
                     /EC_number="2.3.1.266"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008999 -
                     peptide-alanine-alpha-N-acetyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosomal protein S18-alanine
                     N-acetyltransferase"
                     /protein_id="HLJ72408.1"
                     /translation="MSWFRKAAQPVLRPLRNDRAGQCAALHAAAFAHAWSAEEFETLL
                     SAANVSGEAAVDPASGALRGFVLTRIAADEAEILTIAVDSALRRRGVGRALLAAHMAR
                     LPALGAKALFLEVERDNAAALALYAKFGFREVGQRKGYYRKRDGTAATALVLRRDLL"
     gene            6529..7380
                     /locus_tag="VKU03_13905"
     CDS             6529..7380
                     /locus_tag="VKU03_13905"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HLJ72409.1"
                     /translation="MTAFFRAGAAFCAKGGPLVLARLIAALAAAAAIILIGGPIRRLA
                     CWRGWRLGDSLPPLLHRVLCFGLGLRLRVHGQASDARPQLVVPNHVSWLDIVVLGAWR
                     PTEFLAKQEVGAGALARLLVGLQGAVYVERGRRRKIPLVNVEMARRMRAGAPLVLFAE
                     ATTNDGNRILRFKSSHFEAPRLALGGPAGAAIVQPVFIAYSRRGGLPLGRAQRPFVAW
                     YGDMRFFSHLWRFLLDGQVDCDLYCGAPLLVRRGEHRKEIARRAERAVRTLAEAARRG
                     PPRLAEA"
     gene            7481..8908
                     /gene="miaB"
                     /locus_tag="VKU03_13910"
     CDS             7481..8908
                     /gene="miaB"
                     /locus_tag="VKU03_13910"
                     /EC_number="2.8.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654834.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (N6-isopentenyl
                     adenosine(37)-C2)-methylthiotransferase MiaB"
                     /protein_id="HLJ72410.1"
                     /translation="MFDTLDPPLALAAEESRPQDGKKIFVKSFGCQMNVYDSRRMSDV
                     AAGQGYSPTAEIEEADLVILNTCHIREKASEKIFSELGKTRELKERRAAAGRDTTIVV
                     AGCVAQAEGGEITRRQPAVDLVVGPQNYHRLPDLLRQARATPGIVDTDFPIQSKFDHL
                     PAAEPAATRARGVSAFVTVQEGCDKFCSFCVVPYTRGAEFSRPIAQVVAEIASLTAAG
                     VREITLIGQNVNAYHGLGEGGEACDLAELLERAAACPGVERVRYTTSHPTDMSPSLIA
                     AHRDNPALAPFLHLPVQSGSNRVLEAMNRKHTVGDYLDIVAALRAARPDIALSSDFIV
                     GFPGESEAEFEETLALTRKVGFASSFSFKYSPRPGTPGAEMKEQIDEETKARRLALLQ
                     GALEEQRQAFNRAAVGRVFPVLIEKIGRHPGQLIGRSPYMQSVLTEGEPDMIGALAEI
                     EILSVGPHSLRGRIVGAKGVKETPH"
     gene            8956..9942
                     /locus_tag="VKU03_13915"
     CDS             8956..9942
                     /locus_tag="VKU03_13915"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PhoH family protein"
                     /protein_id="HLJ72411.1"
                     /translation="MQPEPRDSKPFETTLAFDDNGLASLVFGHYDQNLAAIERKLGVI
                     ANANGNHVVIKGPKEATEHARRVFEMLYERAKLGQGADLGDVEGAIAEGALQGSLFPN
                     DPDSRPAFQQIKTRKRGVVRARNAAQNLYLSALKHNELVFAEGPAGTGKTWLAVGYAV
                     SLLEQGAVERLILSRPAVEAGEKIGFLPGDMREKVDPYLRPIYDALYEFMDGRMVERG
                     IETGMIEIAPLAFMRGRTLANSLVLLDEAQNATAMQMKMFLTRLGEGSRMIVNGDPSQ
                     TDLPHGEKSGLSEAVALLARLEGVGHVVFRAGDVVRHDLVRRIVAAYEGARR"
BASE COUNT         1687 a         3326 c         3167 g         1769 t
ORIGIN      
        1 ggccagtttc ctcaccaaag ctcttggttg ccaataagcc acgccgggcg atcgcaagtc
       61 acccggaggg ccggagcgaa gcggaggacc cgccatagcg ccgcacaagc ggatatgatc
      121 ttgcacgtag cttgccggcg ccatggcggg ttgacggcga tcggccggcg tggcgccgcg
      181 gcgctgaaag attacgtcca agacgcgcca taacctcaga ctttccccgg acagccctgc
      241 gcaacgcagg gagaagaggt tcgcggctgc tttatttgcg atttcaggcc gattccgata
      301 ggggaaaagc cggttcgcca aacggacgcg tgaaagccat ggcctcgacc gacgtttcgc
      361 cttctcccgc tcgcgcaaga ccgctttcgc cgcatctcac cgtctatcgg tggaccatga
      421 cgatggcgat gtcgatcttt caccgcatca ccggcggggc gctctatgtc ggcacaatcc
      481 tgctcgcctg gtatctgatc gcgctgtcga tggacgaatc ggcgttcggc gtcgtctccg
      541 gctttctgaa ctctatcatc ggtcagatca ttctgttcgg cttcacctgg gcgctgatcc
      601 atcacgcgct cggcgggatt cgtcacgcga tttgggacac gggctacggc atggagaagc
      661 cgatgcgcga ccagctcgca tgtgcgacct tgatcggcgg cgttgtcctg acgatcgtcg
      721 tctggatcat cggctatgcg gtgcgctagg agcggctcat gacgtccgac gacaattcgc
      781 ttcgctctcc tctcgcccgc gtccgctatc tcggctcggc gcgttcgggc gcgcgggaaa
      841 actggctgat gcgggtgacg tcggcggcgt tgatcccgct cgtcatcgcc tttgtctggg
      901 tgatgctgtc gctgctgtcg aaggactata acggcgcgcg cgccgagctc ggccggccgc
      961 tgccggcgat catcgtcatg ctgttcgtgc tggtcggaat ctatcacatg cagatcggca
     1021 tgcgttcgat catcttcgat tatgtgcatg gccgcgccaa ggaatgggcg ctgatcggca
     1081 atctcttctt cgccggcgcg ctggcgctgg cctgcgtcta ttcggtgctg cgcatcggct
     1141 tcgtctgaag gctttaaaat ggcgaccaac ggcaagaacg gggcgagcgc gccggcccag
     1201 aacggcagag cctatccgat caccgaccat acgttcgacg tggtcgtggt cggcgccggc
     1261 ggcgcgggcc tgcgcgccac ggtcggctgc gcgcaaggcg gcctgcgcac cgcctgcgtg
     1321 accaaggtct ttccgacccg ctcgcacacc gtcgccgcgc agggcggcgt cgccgcctcg
     1381 ctcggcaata tggggctgga cgattggcgc tggcacatgt acgacacggt caagggctcc
     1441 gattggctcg gcgaccagga cgcgatcgaa tatctttgcc gccacgcgcc ggccgcggtc
     1501 tacgagctcg accattgggg cgtgcccttc tcgcgcaccg aggacggccg catctatcag
     1561 cgtccattcg gcggcatgac gacggattac ggcaagggca tcgcccagcg cacctgcgcc
     1621 gccgccgacc gcaccggcca cgccatcctg catacgctct acggccaagc gctgaaggcc
     1681 aaggccgagt tcttcatcga gttcttcgcc atcgacttga tccgcgattc cgacggccgc
     1741 gtgcgcggcg tcgtctgcct gaaattggac gacggcacga tccatcgctt ccgcgcccat
     1801 ctcaccgtgc tggcgaccgg cggctatggc cgcgcctatt tctcggcgac ctcggcccat
     1861 acctgcaccg gcgacggcaa tgcgatggct ttgcgcgtcg gcctgccgct gcaggacatg
     1921 gagttcgtgc aattccatcc gaccggcatt tacggcgccg gctgcctgat caccgaaggc
     1981 tcgcgcggcg agggcggcta tctcaccaat tcggagggcg agcgcttcat ggagcgctat
     2041 gcgccctcag ccaaggacct cgcctcgcgc gacgtggtca gccgagccat gacgatggag
     2101 atcagagagg ggcgcggcgt cggcaagctc aaggaccata tctacctcca tctcgatcat
     2161 ctcgacccga aaatcctgca tgagcgcctg ccgggcattt ccgagagcgc ccgcatcttc
     2221 gccggcgttg atgtgaccaa ggagccgatc ccggtcctgc cgaccgtcca ttacaatatg
     2281 ggcggcgtgc cgacgaacta tcacggcgag gtcgtcacca aggcgaacgg cgatcccgat
     2341 aaaatcgtgc ccggcctgat ggcgctgggc gaggcggcct gcgtgtcggt gcatggcgcc
     2401 aaccggctcg gctcgaattc tctgatcgac ctcgtcgtgt tcggccgggc ggcgggcctg
     2461 cgcgcccgag aactcgtcga gcccggcgaa tcgcagcccg aattgccgaa agattcggcc
     2521 gatttcgcct tgtcgcggct cgaccgattc cgccacgcca atggcggcac gccgaccgcc
     2581 gaactgcgcg gccgcatgca gcgcaccatg caggccaatt gcgcggtgtt ccgcaccggc
     2641 gaagttctgc acgaaggcca gaagctaatc catgacgtct ggcgcgccgg cgccgatctc
     2701 aaggtcacgg accgctcgct gatctggaac tccgatctcg tcgagacgat ggagttcgac
     2761 aatctcatcg cccaggcggt cgtcacgatg gacagcgccg ccaaccggct cgagagccgc
     2821 ggcgcgcatg cgcgcgaaga ctatcccaac cgcgacgaca aggaatggat gaagcacacc
     2881 ctggcctgga tcgatccgga gaagcagacc gtcacgatcg actaccggcc ggtgcatacc
     2941 tatacgatga cgaacgacgt gacgtatatc gagccgaagg cgcgggtgta ttgaggaggg
     3001 tcgatgaacg cgctcacgat tgaaaagctc agtccgcaga cctggagcaa tctctccgcg
     3061 cgcgcccgcg cgcaccacaa ctccatcgaa gatgaagccg ccgctatcct cgagtcggcg
     3121 ctgaacgaag gcccaggcaa gagcgagcgg cagcggattg tcgaccgaat tgcggcgatg
     3181 acgcccaagg gcgtggttca agacgatagc acgctgttca ttcgagcgga gagagaccgt
     3241 cagcggtgaa cctaatcatc gacgccagcg tcgcggtgaa gtggttcgtc gaggaggagc
     3301 ggcgcgactt ggcgtgcgac gttcttcgcg acggcttcaa tctgtttgcg ccggatctcg
     3361 cgctcgtcga ggtcgcaaac gctttgcgta ataaagtgcg tttgggtcgg atgaactatg
     3421 aacaggcgcg agcgtcgttg actgtctttc cgggtctctt cagccggctt gttccgcaga
     3481 ttgagacttt tcgcgaggct ttcgaaatag gctgcgccat caatcattcg gcttacgatt
     3541 gcgtttatct tgcatgcgtc aaaatcctta atacagcgct cctgacggat gatgcgacgc
     3601 tctttcagaa ggcgagcgcc ctcgacctcg gcgcgaagac gcttcgattg agcgattgga
     3661 agcctggact cccagctaac acgacggtgt gatccgatgg tccaattcaa ccttcctaag
     3721 aactcgcgca tgaccggagg caagacctgg ccgaagccgg ccggcgccaa gcggacgacc
     3781 gagttccgca tctatcgctg gaatccggac gacggggcca atccgcgcat cgacacgtac
     3841 actgtcgatc gcgacgattg cgggccgatg gtgctcgacg cgctgatctg gatcaagtcg
     3901 aagatcgatt cgaccctgac cttccgccgc tcctgccgcg agggcatttg cggctcctgc
     3961 gcgatgaaca tcgacggcgg caatacgctg gcctgcacgc gcggcatgga cgaggagagc
     4021 aagggcggcg gcccgatcaa gatctatccg ctgccgcatc tcgccgtggt caaggacctg
     4081 gtgcccgacc tctccaattt ctacgcccag cacgcctcga tcgagccctg gctgaagacc
     4141 gtgacgccgg cgccggaaaa ggagcggcgc cagagcccgg ccgatcgcgc caagctcgac
     4201 gggctctacg aatgcatcct ctgcgcctgc tgctccacct cctgcccgag ctattggtgg
     4261 accggcgagc gctatctagg gccggcggtt ctgctccagg cctatcgctg gctgatcgat
     4321 tcgcgcgacg aggcgaccgg cgacaggctc gataatctcg aagatccgtt ccgcctctat
     4381 cgctgccata cgatcatgaa ctgcgcccag acctgcccga aaggcctgaa cccggccaag
     4441 gccatcgccg aaatcaagaa cctgatgatc gagcggcaga tgtgaggcgc tcgctcttcg
     4501 caacgagcgc ctctctgctg gcggccgcgc cagcttcaca tctgtcgcaa ggctctttga
     4561 accgcttcgg gctctcgatc aatgatcgtc agcaacacac gggcttgcgc atccgggtcg
     4621 cggaggccct gctcccaatt tttaagagtg ccaggcgata tgccgaagcg tgcggcaaag
     4681 gcgttttggc tgaggcccaa tttagcgcgg atagctttca cgtcgatcct gtccgggaca
     4741 tggacacgat agctcgcggg atcggcctcg cctttcgcga tcgcgcgcgc ttctttagcc
     4801 gccctgataa gatgctggcc gagcttggtc acggggatgt ccttaatttg tttaccttgg
     4861 ctgcgaggcc ggcgcgataa tcgtcggcga gcgaggccaa ttcggccttg agcgcgttcc
     4921 gttccgcctg cgacagatcg gctctctctc ccttggaaat cagcgcaaaa gccggcacct
     4981 ctttcgcggc atagtacgtg acgacgcgat agccgcccga cttgcctttg ccgcgcccac
     5041 cgatccgcac tttgcgcgcg ccgccggttc cgacgatcag gtctccggct tccggatcgg
     5101 acgcgataat cgcaacgatc cgcgcggctc cttcgatcgt cacgccggcc gagttgagat
     5161 ccccgagata gctcggagtc tcgatgactg ggtgcataag ggctatatac gtcaatgacg
     5221 tatagaaatc aagacgcgcg cttcgctctc tcgccagcgc ccgaaaagcg aatcgaacga
     5281 cttctcttct cggccagctt ccttcttctc ctcttccttc gggtgagata cctagacccc
     5341 atgcgcattc tcgccatcga cacctcctgc ggcgcggctt cggcctgcgt cgccgaaagc
     5401 ggcgcggccg agccggtcgc ggtcgagacg ctgccgatgc agcgcggcca cgccgaggct
     5461 ttggcgccga tgctgcaaag gctgatggcc ggcgtcgagg gcggctttgc ctcgctcgac
     5521 cgaatcgcgg ccacggtcgg cccgggctcc ttcaccggca tcaggatcgg cctcgccacg
     5581 gcgcgggcga tcggcctcgg cctttcgctt ccggtggtcg gcgtctcttc gctcgtggct
     5641 ttcgccggcc cgcttctcgc cgacccgaag cccggggtta tcgccgcctc gatcgacgcc
     5701 aagcatggca aggtctatct gcaattgctc gaggcgaacg gccggccgct cctgccgccg
     5761 cgcctcgccg cattgcgcga cgccatccgc ctgatcggcg cgggaccggt gcggcttacc
     5821 ggcaacggcg cggcgctgct cgccgccgaa gcggcgcgga tcggccttgc ggcggaaatc
     5881 gccggcaatc tcgattatcc ggacatcgtc gcggtggcgc ggctcggtct cgtcgccgag
     5941 cccagccagt cgccgccgcg cccgctctat ctgaaagccg ccgacgccca ggcgccggaa
     6001 aatcccgccg tcgcccgcgc cggttcttga aagccggatc gagatcgaaa gcatgtcctg
     6061 gtttcgaaag gccgcccagc cggtgctgcg gccgctgcgc aacgaccgcg ccggccagtg
     6121 cgcggcgctg cacgctgcgg ccttcgccca tgcctggtcg gcggaggaat tcgagacgct
     6181 gctctcggcg gccaatgtct ccggcgaggc ggcggtcgat ccggcgagcg gcgcgctgcg
     6241 cggcttcgtc ctgacccgga tcgctgcgga cgaagccgaa atcctgacca tcgccgtgga
     6301 cagcgcgctg cgccggcgcg gcgtcggccg cgctctgctc gccgcccata tggcgcggct
     6361 gccggcgctc ggcgccaagg cgctgttcct cgaagtggag cgcgacaatg ccgcggcgct
     6421 ggcgctttac gccaaattcg gcttccgcga ggtcggccag cgcaagggct actatcgcaa
     6481 gcgcgacgga accgccgcga ccgcgcttgt cctgcgccgc gatttgctat gacggctttt
     6541 ttccgcgccg gcgccgcctt ctgcgccaag ggcggcccgc tcgtcctggc gaggctgatc
     6601 gccgccctcg ccgcggcggc cgcaatcatt ctgatcggcg gcccgatccg ccgcctcgcc
     6661 tgctggcgcg gctggcggct cggcgacagc ctgccgccgc tgctccacag ggtcttatgc
     6721 ttcgggctcg gcctgcgcct gcgcgtccat ggccaggcga gcgacgcccg gccgcaactc
     6781 gtcgtcccca atcacgtttc gtggctggat atcgtggtcc tcggcgcgtg gcggccgacc
     6841 gaattcctgg ccaaacagga ggtcggcgcg ggcgcgctgg cgcggctgct ggtcggcttg
     6901 cagggggcgg tctatgtcga gcgcggcagg cgccggaaaa ttcccctggt gaacgtcgaa
     6961 atggcccgca ggatgcgcgc cggcgcgccg ctggtgctgt tcgccgaggc gacgaccaat
     7021 gacggcaacc gcatcctgcg cttcaaatcc tcgcatttcg aagcgccgcg cctggcgctt
     7081 ggcggcccgg ctggggccgc gatcgtccag ccggtcttca tcgcctattc ccggcgcggc
     7141 ggcctgccgc tcggccgggc tcagcggccg ttcgtcgcgt ggtatggcga tatgagattt
     7201 ttcagccact tatggcgctt cctgctggac ggccaagtcg attgcgacct ctattgcggc
     7261 gcgccgctcc tggtccggcg gggcgagcat cgcaaggaga tcgcccggcg cgccgagcgg
     7321 gcggtgcgca cgctcgccga ggcggcccgg cgcggtccgc cgcgcctggc ggaggcttga
     7381 gcgcttcgat ttgaacgccc cacgccgaaa tcgcctattc tcgccgggcc gcaaaggctc
     7441 taaatgaagg tggatcggtc cggagaccct gttggcggcc ttgttcgata cgcttgaccc
     7501 cccgttagcc ctcgcggccg aagagtcgcg gccgcaagac ggcaaaaaaa tcttcgtcaa
     7561 gtcattcggc tgtcagatga atgtctatga ttcgcggcgc atgagcgatg tcgctgcagg
     7621 ccaaggctat tcgccgaccg ccgagatcga ggaggcggac ctcgtcatcc tcaacacctg
     7681 ccatatccgc gaaaaggctt ccgagaagat tttttccgag ctcggcaaaa cgcgcgagct
     7741 caaggagcgg cgcgccgccg cgggccgcga cacgacgatc gtcgtcgccg gctgcgtggc
     7801 gcaggccgag ggcggcgaaa tcacccgccg tcagcccgcc gtcgacctcg tggtcggacc
     7861 gcagaactat caccgcctgc ccgacctgct gcgccaggcg agagcgacgc cgggaatcgt
     7921 cgacaccgat ttcccgattc agagcaaatt cgaccatctg cccgccgccg agccggcggc
     7981 gactcgggcg cgcggcgtca gcgccttcgt caccgtgcag gagggctgcg acaaattctg
     8041 ttcgttctgc gtcgtgccct atacgcgcgg cgccgaattt tcgcgtccca tcgcccaggt
     8101 cgtcgcagaa atcgccagtc tcacggcggc cggggtgcgc gaaatcacgc tgatcggcca
     8161 gaacgtcaac gcctatcatg ggctcggcga gggcggcgag gcatgcgacc tcgccgaact
     8221 cctggagcgg gcggcggctt gcccaggcgt cgagagggtc cgctatacga cctcgcaccc
     8281 gactgacatg tcgccgtcgc tgattgcggc gcatcgcgac aatccggctc tcgctccctt
     8341 cctgcatttg ccggtgcaat cgggctcaaa ccgggtgctc gaagcgatga accgcaagca
     8401 tacggtcggg gattatctcg acatcgtcgc cgccttgcgc gcggcgcggc cggacatcgc
     8461 cttgtcgtcg gatttcatcg tcggctttcc cggcgagagc gaagcggaat tcgaagagac
     8521 gctggcgctg acgcgcaagg tcggcttcgc ctcgtcgttc tcgttcaaat attcgccgcg
     8581 ccccggcacg cccggcgccg agatgaaaga gcagatcgac gaagagacca aggcgagaag
     8641 gctggcgctt ctgcaagggg cgctcgaaga gcagcgccag gctttcaacc gcgccgcggt
     8701 cgggcgcgtc tttccggtcc tcatcgaaaa aatcggccgc catcccggtc aattgatcgg
     8761 ccgctcgccc tatatgcagt cggtgctgac cgagggcgag ccggacatga tcggcgcgct
     8821 ggccgagatc gaaattctga gcgtcggccc gcattcgctc agaggccgca ttgtcggcgc
     8881 gaaaggggtt aaggagactc ctcattgaga acgcaggatc gaacgatcgc caaaatccct
     8941 ccggcgaacg ccgacatgca acccgagccg cgcgattcca aacctttcga gaccacgctc
     9001 gccttcgacg ataacggctt ggcttccctc gtcttcggcc attacgacca gaatcttgcc
     9061 gcgatcgagc gcaagctcgg cgtcatcgcc aacgccaacg gcaatcacgt cgtcatcaag
     9121 gggccgaaag aggcgaccga gcatgcccgc cgggtcttcg aaatgctcta tgagcgggcc
     9181 aagctcggcc aaggcgccga tctcggcgac gtcgaaggcg cgatcgccga gggcgcgctg
     9241 cagggcagcc tgtttccgaa cgatccggat tcccgccccg cctttcagca gatcaagacg
     9301 cgcaagcgcg gcgtggtgcg ggcccgcaac gccgcgcaaa atctttatct ctccgccttg
     9361 aagcacaacg aattggtctt cgcggaaggc ccggcgggca ccggcaagac ctggctcgcg
     9421 gtcggctacg ccgtctcgct gctcgaacag ggcgcggttg agcggctgat cctgtcgcgg
     9481 cccgcggtgg aggccggcga gaagataggc ttcctgccgg gcgatatgcg cgagaaagtc
     9541 gatccgtacc tacgcccgat ctatgacgcc ctctacgaat tcatggacgg aaggatggtc
     9601 gagcgcggca tcgagaccgg catgatcgag atcgcgccgc tcgcctttat gcgtggccgg
     9661 accctggcca attcgctggt cctgctcgac gaggcgcaga acgccaccgc catgcagatg
     9721 aaaatgttcc tgaccaggct cggcgaaggc tcgcgcatga tcgtcaacgg cgatccgagc
     9781 cagaccgatc tgccgcacgg cgagaaatcc ggcctgagcg aggcggtggc gctgctggcg
     9841 cgcctcgaag gcgtcggcca tgtcgtcttc cgcgccggcg acgtggtgcg ccacgatctc
     9901 gtccgccgca tcgtcgccgc ttatgagggc gcgcggcgat gaggattac
//
LOCUS       DATGJW010000461         2582 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_403664, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000461 DATGJW010000000
VERSION     DATGJW010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2582)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2582)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2582
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_403664"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            134..994
                     /locus_tag="VKU03_13920"
     CDS             134..994
                     /locus_tag="VKU03_13920"
                     /EC_number="4.2.2.n1"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016798 - hydrolase activity, acting on
                     glycosyl bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lytic transglycosylase domain-containing
                     protein"
                     /protein_id="HLJ72412.1"
                     /translation="MMAIAVAVLASALTIVQADAPSLAEPVPTPSLNGGPNFCRVLDA
                     LAVQNDLPMPFFARLIWQESKFDPHAQSSAGAQGVAQFMPFTAASRGLLDPFEPTAAL
                     RESASYLRELRNTFGNLGLAAAAYNAGPYRVSQWLAHKASLPDETIAYVQIVTGHSVS
                     DWAGPDAAQWEASLPLDAGCDDVRQMLTLPPAGPSGPPSAPWKPWGVQLAGAWTRGPV
                     LAAFERLRRRFADILEDRPPLILRVRPAFAPALHYVVRIGEDSAAAANRLCGRLRAAG
                     GVCDVVRNPR"
     gene            1198..2289
                     /locus_tag="VKU03_13925"
     CDS             1198..2289
                     /locus_tag="VKU03_13925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2778 domain-containing protein"
                     /protein_id="HLJ72413.1"
                     /translation="MQGTLATFEQDSLDGGRDLRRLFLQTAPACAAAAALAACISAWA
                     LRARPAAAPDFTPPPVILPAAPPAPAVPPPTEAASNPYGALSNPYAPFGALVDRAFFA
                     EAGPAVAPPAGSQQASAEILPPAPAEAAPPAAAIPTPPQREAAEAGAPPLPPRRPAEL
                     DALAPAPPRVAAPAQSVATAPAAPADNRNLFEKLFDGGKPTSAAVAAAKPPEGRGGVR
                     GAGLLAALFASSGQPAGYDKWTAVYDISARTVYMPDGTRLEAHSGLGDRLDDPRFVNE
                     RMRGATPPHLYELEPREASFHGVQALRLIPIGNGDLFGRAGLLAHSYMLGPKGDSNGC
                     VSFKDYDAFLRAYQNGQIKRLAVVARLNG"
BASE COUNT          375 a          942 c          834 g          431 t
ORIGIN      
        1 tcattttgga cgatcgccaa gcgtcgaatg tggggtcttc cccgccgcgc tccagttgcg
       61 ccgccgcgcc gcattcgcct gagcgagcgc ggcggaatcc cttccagcgt ttgccgcctc
      121 gcctccagtc tccatgatgg ccattgcagt cgctgtcctc gcctcggcgc tgaccatcgt
      181 ccaggccgat gcgccatccc tggccgagcc ggtccccacg ccttctctga acggcggtcc
      241 caatttttgc cgcgtgctgg acgccctcgc cgttcaaaac gatctgccga tgcccttctt
      301 cgcgcgcctg atctggcagg aaagcaaatt cgacccgcac gcgcagagct cggccggcgc
      361 gcagggcgtc gcccaattca tgccctttac cgccgcctcg cgcggcctgc tcgatccgtt
      421 cgagccaacc gccgccctgc gcgaatcggc gagctacctc cgcgagcttc gcaacacgtt
      481 cggaaatctc ggcctcgccg ccgccgctta caacgccggg ccctaccggg tgagccaatg
      541 gctggcccat aaggcctcgt tacccgatga aacgatcgct tatgtgcaaa tcgtcactgg
      601 ccattcggtg tcggactggg cgggcccgga cgccgcccaa tgggaggcga gcctgccgct
      661 cgatgcggga tgcgacgacg tcaggcagat gctgaccctg ccgccggcgg ggccgtccgg
      721 gccgcccagc gcgccctgga aaccctgggg cgttcaattg gccggcgcct ggacgcgagg
      781 cccggtcctt gcagccttcg aacgcctgcg ccgacggttc gccgacatat tagaggaccg
      841 cccgccgctg attttgcggg tgaggcccgc cttcgcgccg gctctccatt atgttgtgcg
      901 gatcggcgag gattcggcgg ccgccgccaa tcgcctctgc ggccgcctga gagcggcggg
      961 cggggtatgc gatgtggttc gcaacccgcg ctgactggcg gcggccgacg tgttttggcc
     1021 gctttctttg gacagggttt gcccggcttc gatcgaggcg aagctggatt gggggattgc
     1081 gcgcgggagg cgtttctgaa aagactggcc ttgaaaagcg gtctgcgtta attgaagcta
     1141 aactcctcgc ctataattca attcggggtc ataaagaagc gtaagcgcta acgaggaatg
     1201 caggggacac tcgcgacatt cgagcaggat tcgctggatg gcggccgcga cctgcgccgg
     1261 ctcttcttgc agaccgctcc cgcctgcgcg gcggcggcgg ccttggccgc ttgcatcagc
     1321 gcctgggcgc tccgagcgcg gccggcggcg gcgccggact ttacgccgcc gcccgtcatc
     1381 cttccggcgg cgccccccgc cccggccgtt ccgccgccga ctgaggctgc atcaaatcct
     1441 tatggcgcgc tcagcaatcc ctacgcgccg tttggcgcgc tggtcgatcg ggcgtttttc
     1501 gccgaggccg ggccggccgt tgcgccgccg gccggctcgc aacaggccag cgccgaaatc
     1561 ctcccgcctg ctccggccga agccgccccg cccgccgccg cgatcccgac gccgccgcag
     1621 cgcgaggccg ccgaagcggg cgcgccgcct ttgccgccgc ggcggccagc ggaattggac
     1681 gcgctcgcgc cggcgccccc tcgggtcgct gcgcccgccc aaagcgtcgc cacggcgccc
     1741 gcagcgccag ccgacaatcg caatctcttc gaaaagcttt tcgacggcgg aaagccgacc
     1801 agcgcggccg tcgccgcggc caagccgccg gaaggtcgcg gcggcgtgcg gggcgcgggc
     1861 ctgctcgccg cgctcttcgc ctcttccggc cagccggccg gctacgacaa atggaccgcc
     1921 gtctacgaca tttcggcgcg caccgtttat atgcccgacg gcacccggct ggaggcccat
     1981 tccggcctcg gcgaccggct cgacgatccg cgcttcgtca acgagcggat gcgcggcgcg
     2041 acgccgcccc atctctatga gctcgagccg cgcgaagcgt cgttccatgg agtgcaggcg
     2101 ctgcggctca ttccaatcgg caacggcgac cttttcggac gcgccggcct gctcgcccat
     2161 tcctacatgc tcggcccgaa gggcgactcg aatggctgcg tctcgttcaa ggactatgac
     2221 gccttcctgc gcgcctacca gaatggccag atcaagcgcc tcgccgtcgt cgcgcggttg
     2281 aacggctaag cccgcccgca acgcgtcagg cggatcgtcc aaggtcaggc caaaagcctc
     2341 atggcgcgcc cttaggatca gcctatttat ctcgagtcag cgcaaatgcc ggcggcatgc
     2401 gcgttttccg catatggttc ggcgagcgaa gtcggccccg ccgaggcgcg cctggcgggg
     2461 ccgaccgagc gacgcgccgt gtgggaggcc cggcgcaaga cgccggatcg atcggcgatc
     2521 gcgcggcaag tccgcgctcg atctttgcaa ccgccaggcg aacatgcacg atcacgttcc
     2581 ct
//
LOCUS       DATGJW010000462         5558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_90395, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000462 DATGJW010000000
VERSION     DATGJW010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5558
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90395"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(23..682)
                     /locus_tag="VKU03_13930"
     CDS             complement(23..682)
                     /locus_tag="VKU03_13930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018854760.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HLJ72414.1"
                     /translation="MRRIIAAAFVSLDGVMQAPGGPDEDPTGGFAFGGWTAPHFDEAL
                     GASMGEIFGRPFDLLLGRKTYDIFAAHWPYVADPNDPIAATFNDVRKYVASRAKPNLT
                     WRNSQWLGDDVVASLKKLKSEDGPDLLVQGSSDFIQTLWRNRLIDEFSVLIFPIVLGK
                     GKRLFGGGAVPGGLKLVKAKSYPTGVIVANYAPDGDVKTGDFQLAEPSEAELTRRRNL
                     T"
     gene            complement(785..2047)
                     /locus_tag="VKU03_13935"
     CDS             complement(785..2047)
                     /locus_tag="VKU03_13935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013891936.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tm-1-like ATP-binding domain-containing protein"
                     /protein_id="HLJ72415.1"
                     /translation="MSDAPTILLIGAADTKSPELEFLKTCIAEAGGDTLFMDVGVLAN
                     GALAADFANGEVAAAAGATLAGLAAFGDENRAVAKMAEGAAILARRAYDARRIDGMLA
                     LGGTMGTDLALDVAAALPLGAPKCIVSTVAYSHLIPPERVPPDLMMMLWAGGLYGLND
                     ICRSALAQAAGAIVGACRAARPPRGGRPLIGMTSLGKSCLSYMETLLPALEARGYDVA
                     VFHTTGMGGRAFEALAAEGRFAAVMDFSLQELANHFSDSVVSAGPDRLEAAGRRGVPQ
                     LVAPGAVDMIDFPTWRPGTKASEARNYHAHNRLLASVTATAEERRGVARLISQKLAAA
                     KGPTAYLLPLGGVQGWDRPGGPLHQPEALAAFLDEARRTAPPSVALVEVDAHINDAAF
                     AEAALAIFDKWARDGLAPAATAQKVAEK"
     gene            complement(2067..2831)
                     /locus_tag="VKU03_13940"
     CDS             complement(2067..2831)
                     /locus_tag="VKU03_13940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006203981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IclR family transcriptional regulator"
                     /protein_id="HLJ72416.1"
                     /translation="MSTVEKALSLLDKFDRSTPELGLSEIAKQSGFDKATTRRLLLSL
                     AAGGFVEQDSESRLYRLGPSLLRLARLREAAFPFVDVSRPIVDDLSAETGETVHVSAL
                     AAAALSTIYVRESARAHRVAVALGTRLPFNCTASGVVCLAFGTENLRRAAFAQPLVKL
                     TRHSIASVAELKRRVAEARQSGFAANDQGFEDGVSSVAAPILAASGEAVGAISIASPT
                     ARVDTKTIERHGAAVRAAAARIAAGLGARPQRLKTA"
     gene            3049..4437
                     /locus_tag="VKU03_13945"
     CDS             3049..4437
                     /locus_tag="VKU03_13945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601635.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transaminase"
                     /protein_id="HLJ72417.1"
                     /translation="MTGATTRERTRIDRLIDREQARFAANHPKSRAAWAEGRRHFLYG
                     GPSHWMRRWAGGFPLYVESAEGARVKDIDGFDYVDFCLGDTGGMCGHAPEAVTAAISR
                     QLARGATTMLPTEDSLWVGAELARRFALPYWTLTTSATDANRAAIRIARMITGRDKVL
                     VFSRCYHGGVEEAHVELVDGRMALRNQIHPNGVDHRRVSKVVEFNDAPALAAALDDRD
                     VACVLAEPVMTNFGMIPPEAGFHAALREATRRTETLLILDETHTLSSGPGGYAREHGL
                     DPDMLTIGKAVAGGLPCGAFGVSQDVAERMWRVAPFVDPRERQSAHLGMGGTLAGSAL
                     SVAAMRAVLSEVLTPSAFATMIARAGRLAEEAGRVIAKHGLAWHVTRVGARSEIMFAP
                     HPPRNGAEAAAMRQGRLETFLHAFYLNEGVLATPFHTMLLTCPATGESDVDRHAEVFD
                     RFAAMAIAEGVA"
     gene            4434..5231
                     /locus_tag="VKU03_13950"
     CDS             4434..5231
                     /locus_tag="VKU03_13950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007601636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72418.1"
                     /translation="MTAPAFSLAGKTALVTGAGQGIGAAIVTRFAEAGARVVAANRTV
                     EQARALAAALARRGLNVTAEAFDDLSRAGLTRLVESAARDGLDILVHNAGGCLWAGLD
                     EMSEVALDAALDVNLRACFWLAQAAIPHIRAAGGGRILVTSSVTGPRAAMVRAAHYAA
                     AKAGVNGFIRSAALELARDRITVNGVEPGFIAKPGRGELGGAETMARIAHYIPLGAMG
                     EADDVAYAMLYLASPQAKYVTGQTIVVDGGALLPETGYAMDAARRAS"
     gene            5228..>5558
                     /locus_tag="VKU03_13955"
     CDS             5228..>5558
                     /locus_tag="VKU03_13955"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72419.1"
                     /translation="MTGRLAGRKALIAGAGTGIGRATAERFAKEGAEIVLFGLGGATL
                     AVAAEACGGRAAHGDVTSRADIAAAIAACGARLDILVNAVGFIQPDAPLTIADESWER
                     IFSVNVGG"
BASE COUNT          861 a         1863 c         1955 g          879 t
ORIGIN      
        1 ctcgcaatga caaacggtgc gctcaggtca gattgcggcg gcgcgtcagc tcggcttcgc
       61 tgggctcagc cagttggaag tcgccggtct tgacgtcgcc gtccggcgcg tagttcgcga
      121 cgatcacgcc ggtcgggtac gacttcgcct tgacgagttt caggccgccc ggaaccgcgc
      181 cgccgccaaa caggcgcttg cctttgccga ggacgatcgg gaagatcagc acgctgaatt
      241 cgtcgatcag gcggttcctc cacaaggtct ggatgaaatc gctcgaccct tggacgagca
      301 ggtcggggcc gtcttcgctc ttcagctttt tcagcgacgc gacaacgtcg tcgcccagcc
      361 attggctgtt gcgccaggtc aaattcggct tggcgcgcga ggcgacatat ttccttacat
      421 cgttgaaggt tgcggcgatc gggtcgttcg gatcggcgac atagggccaa tgcgcggcga
      481 aaatatcgta ggtcttgcgg ccgagcaaca agtcgaaggg ccggccgaaa atttcgccca
      541 tcgaggcgcc aagcgcttcg tcgaaatgcg gcgcggtcca gccgccaaac gcgaagccgc
      601 cggtcgggtc ttcgtctggc ccgcccgggg cttgcatgac gccgtcgagg ctgacgaaag
      661 cggcggcgat gatcctgcgc atggcgaatc tcctgcttca agcgcgtttc gatgccccga
      721 ggacggatcg cgcttcgccg ttccgacacg gccggtcaaa aattgtggcc tcggctcagg
      781 ctgctcattt ctccgcaacc ttctgcgccg tcgccgcggg cgccagcccg tcgcgcgccc
      841 atttgtcgaa aatcgccaac gccgcttcgg cgaaggcggc gtcattgata tgggcgtcga
      901 cttcgacgag cgcgaccgag ggcggcgcgg tgcggcgcgc ttcgtcgagg aatgcggcga
      961 gcgcttcggg ctgatgcagg gggccgccgg gccggtccca gccttgcacg ccgccgagcg
     1021 gcaggagata agcggtcggg cctttcgcgg cggcgagttt ttgcgagatg agtctcgcaa
     1081 cgccgcgccg ctcctcggcg gtggccgtca ccgaggcgag aagacggtta tgcgcatgat
     1141 agttgcgcgc ttcggacgct ttcgttccgg gccgccaggt cggaaaatcg atcatgtcga
     1201 cggcgccggg cgcgacgagc tgcggcacgc cgcggcggcc cgccgcttcg agccggtccg
     1261 ggccggcgct gaccaccgag tcggaaaaat gattggcgag ctcttgcagg ctgaaatcca
     1321 tcaccgcggc gaaacgtcct tccgcggcca gcgcttcgaa ggcgcgcccg cccatgccgg
     1381 tcgtatggaa gacggcgacg tcgtagccgc gcgcctcaag cgcaggcagc aaagtctcca
     1441 tataggagag gcagcttttg ccgagcgagg tcatgccgat cagcggcctt ccgccgcgcg
     1501 gcgggcgcgc cgcgcggcag gcgccgacga tcgcgccggc cgcctgggcg agcgccgatc
     1561 ggcaaatgtc gttgaggcca tagaggccgc cggcccagag catcatcatc aagtcgggcg
     1621 gtacgcgctc cggcggaatg agatgcgaat aggcgacggt cgagacgatg cattttggcg
     1681 cgccgagcgg cagcgccgcg gcgacgtcga gggcgaggtc cgtacccatc gtgccgccga
     1741 gcgccagcat gccgtcgata cgccgcgcgt cgtaggcgcg gcgcgcaagg atcgccgcgc
     1801 cctccgccat tttggcgacg gcgcggtttt cgtcgccgaa agcggcgagg ccggccagcg
     1861 tcgcgccggc cgcggcggcg acttcgccat tggcgaaatc ggcggcgagc gcgccattcg
     1921 cgagcacgcc gacatccatg aagagggtgt cgccgccggc ctcggcgatg caggtcttga
     1981 gaaattcgag ctccggcgat ttggtgtccg ccgcgccgat caacaaaatg gtcggagcat
     2041 cgctcatggc ccctcgcaaa taggcgtcaa gccgtcttga ggcgctgcgg ccgcgcgccg
     2101 aggccggcgg cgatccttgc cgcggcggcg cgcacggcgg cgccgtggcg ctcgatggtt
     2161 ttcgtgtcga cgcgcgccgt cgggctggcg atcgagatcg cgccgaccgc ttcgccggaa
     2221 gcggcgagaa tcggcgcggc gacgctcgat acgccgtctt cgaagccttg gtcgttggcg
     2281 gcgaagccgg attgccgcgc ctcggcgacg cgccgcttca attcggcgac gctggcgatc
     2341 gaatggcgcg tcagcttgac gagcggctgg gcgaaggccg cgcggcgcag gttctccgtg
     2401 ccgaacgcca ggcagacgac gccggacgcc gtgcaattga acggcaggcg cgtgccgagc
     2461 gccacggcga cgcggtgggc gcgagcgctt tcgcgaacat agatcgtcga cagcgccgcg
     2521 gcggccaacg ccgagacatg caccgtctcg ccggtctcgg ccgacaagtc atcgacgatc
     2581 ggccgagaga cgtcgacgaa aggaaacgcc gcctccctca gccgcgccag gcgcagcagg
     2641 ctcggcccta gccgatagag gcgcgattcc gaatcctgtt cgacgaagcc gcccgcggcg
     2701 agcgacagca acaggcggcg cgtggtcgct ttgtcgaagc cggattgctt ggcgatctcg
     2761 ctgaggccga gctccggcgt cgaccggtcg aacttgtcca aaagcgacag cgccttttcg
     2821 accgtgctca tcgtttggct cgtccccagc cgcgcgggcg cccccgagcg ggcgcccaac
     2881 ttgcttcttg actcgcgccg cccgcgtttg caagtctata ttcgaaataa aggttcatta
     2941 tgtgaaccta cggcgaggcg gctcgaggat gcggcggaac ggaatcgatc ggatcggcga
     3001 gggccttgct ccgccgcggc ggcgcgtccg tacggatgtg cgcaccgcat gaccggcgcg
     3061 acgacgagag agaggacgcg catcgaccgg ctgatcgacc gcgagcaggc gcggttcgcc
     3121 gccaatcatc ccaaatcgcg cgcggcctgg gccgagggcc ggcggcattt cctttacggc
     3181 ggtccgtcgc actggatgcg gcgctgggcc gggggcttcc cgctctatgt cgaaagcgcc
     3241 gagggcgcgc gcgttaagga cattgacggg ttcgactacg tcgatttctg cctcggcgat
     3301 acgggcggca tgtgcggcca cgcgccggag gcggtgaccg ccgcaatcag ccggcagctc
     3361 gcgcgcggcg cgaccacgat gctgccgacc gaggacagct tgtgggtcgg cgccgagctc
     3421 gcgcgccgct tcgctctgcc atactggacg ctgacgacct cggcgaccga cgccaatcgc
     3481 gccgcgatca ggatcgcgcg gatgatcacc gggcgcgaca aggtcctcgt cttctcccgc
     3541 tgctaccatg gcggcgtaga ggaggcgcat gtcgagctcg tcgacgggcg gatggctttg
     3601 cgcaaccaaa tccacccgaa cggggtcgat catcgccgcg tttcgaaagt cgtcgagttc
     3661 aatgacgcgc ccgcgctggc ggcggccttg gacgatcgcg acgtggcctg cgtcctcgcc
     3721 gagccggtca tgaccaattt cggcatgatc ccgcccgagg ccggctttca cgccgctctg
     3781 cgcgaggcga cgcggcggac ggagacgctg ctgatcctcg acgagacgca cacgctgtcc
     3841 agcgggcccg gcggctatgc gcgcgagcac gggctcgatc ccgacatgct gacgatcggc
     3901 aaggcggtcg cgggcgggct gccatgcggc gctttcggcg tttcacagga tgtcgcggag
     3961 cgcatgtggc gcgtcgcgcc attcgtcgat ccgcgcgagc ggcagagcgc tcacctcggc
     4021 atgggcggaa cgctcgccgg ctcggcgctg agcgtcgccg cgatgcgcgc cgtactctcc
     4081 gaagtactca cgccgtcggc ctttgcgacg atgatcgcga gagccggccg gctcgccgag
     4141 gaggccgggc gcgtcatcgc caaacacggc cttgcttggc atgtcacccg cgtcggcgcc
     4201 cggtcggaaa tcatgttcgc gccgcatccg ccgcgcaacg gcgccgaggc ggccgcgatg
     4261 cggcagggcc gactggagac ttttctgcac gctttctacc tcaacgaagg cgtgctggcg
     4321 acgccgttcc atacgatgct gctgacgtgc ccggcgacgg gcgagagcga tgtcgaccgc
     4381 cacgcggaag ttttcgaccg cttcgcggcc atggcgatcg cagagggcgt cgcatgaccg
     4441 cgcctgcttt cagcctcgcc ggcaagaccg ccctggtgac aggcgccggg cagggcatcg
     4501 gcgccgccat cgtgacccgc ttcgcagagg ccggcgcgcg cgtcgtggcg gcgaaccgaa
     4561 cggtcgaaca ggcgcgcgcc ctggccgctg cgctggccag gcgcggcctc aatgtgaccg
     4621 cggaagcttt cgacgatctc tcccgcgccg gactgaccag gctggtcgaa agcgctgcgc
     4681 gcgatgggct cgacatcctc gtccacaatg ccggcggatg tctctgggcc gggctcgacg
     4741 agatgagcga ggtggcgttg gacgccgcgc tcgacgtcaa tctgcgcgcc tgtttctggc
     4801 ttgcgcaggc ggcgattccg catatccgcg cggctggcgg cggacgtatt ctcgtcacct
     4861 cctccgtcac cggtccgcgc gccgcgatgg ttcgcgccgc ccattatgcg gccgccaaag
     4921 ccggcgtgaa cggcttcatc cgctccgcgg ctttggagct tgcccgcgat cgcatcaccg
     4981 tcaacggcgt cgagccgggc ttcatcgcca agcccggccg cggcgagctc ggcggcgccg
     5041 agacgatggc gaggatcgct cattacattc cgctcggcgc aatgggcgaa gccgacgacg
     5101 tcgcttacgc gatgctgtac ctggcgtcgc cgcaggcaaa gtacgtcacc gggcagacga
     5161 ttgtcgtcga tggcggcgcg ctgctgcccg agaccggcta tgcgatggat gcggcgagga
     5221 gggcgtcatg accggacgcc tcgccggccg caaggctctg atcgccggag cggggacagg
     5281 gatcggccgc gcgacggccg agcgtttcgc caaggaaggg gcggaaattg ttctgttcgg
     5341 cctcggcggc gcaaccctcg ccgtcgccgc agaggcctgc ggcgggaggg ccgcgcatgg
     5401 cgacgtgacc agccgcgccg atatcgccgc ggcgatcgcc gcctgcggcg ctcggctcga
     5461 tatcctggtc aacgccgtcg ggttcatcca gcccgatgcg ccgctcacca tcgcggacga
     5521 aagctgggag cggatttttt cggtcaatgt cggcggcg
//
LOCUS       DATGJW010000463         6495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1538538, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000463 DATGJW010000000
VERSION     DATGJW010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6495
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538538"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VKU03_13960"
     CDS             complement(<1..254)
                     /locus_tag="VKU03_13960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72420.1"
                     /translation="MLKTVLLTGAIAVSGGTAALANSPPIADNFQPFMWRSPQVIGQP
                     MSAGQLGMLDRVSKAPVAATAAAYVPRYASMPVAPTSYSAL"
     gene            complement(345..1664)
                     /locus_tag="VKU03_13965"
     CDS             complement(345..1664)
                     /locus_tag="VKU03_13965"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ72421.1"
                     /translation="MSSLRRAVLIWTTVLLAAIGAIAFGVSYKIGRQQAAEFLDGQLR
                     QIALNAGGSVGETFTPSVDQDEEDRLVVNVWSVPDGRLLTSFGGLLPQFGRSGLGTIQ
                     AGGESWRVFLARDSRRIVQVGQKMVVRDEIAATAGLEAGLPILIAIPLGWLVLSFALG
                     RVLRPLTIVAEAIASRKPESTEPIPLDHAPSELRPVLVAMNDLTTRLGRMVLRQRRFV
                     SDAAHELRTPLTALRIQIDNLAAETAGGDPEAFDEMKRGLDRASALVDQMLKLARLDA
                     SEAVRRDERVDLTDLVSACVAESLPLADAKGVDLSLDASPTGALLASREDLKLLVANL
                     LQNAIRYTGAKGRVDVRVFAQKGLATVEILDTGPGIPDAELPRVFERFYRAAPSDVEG
                     SGLGLAIAQSVARKYGFELTLANRSDRSGLCARARISTARLDMLTLP"
     gene            complement(1661..2326)
                     /locus_tag="VKU03_13970"
     CDS             complement(1661..2326)
                     /locus_tag="VKU03_13970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009797658.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLJ72422.1"
                     /translation="MRILLIEDDPMIGRGLRRSLGREGASVDWVRDGAEGAEAAASGE
                     HDMILLDLGLPGIGGLEVLRRMRAAGVRTPVVIVTARDEIEDRVRGLDLGADDYVVKP
                     FATEELLARIRAVSRRRSGAAESLLHAGDLCLNLAEHTLSRGENGVVLPAREFALMRA
                     LMERPGAILSRAQLEDRLYGWGEEVESNAVDVLIHSVRKKFGKNIIRNIRGAGWVVSR
                     NGS"
     gene            complement(2357..3037)
                     /locus_tag="VKU03_13975"
     CDS             complement(2357..3037)
                     /locus_tag="VKU03_13975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLJ72423.1"
                     /translation="MAQQVLIVDDEPQIQRFLGPALNAAGYEALKAASGREALRLIAV
                     AAPDIVVLDLGLPDMDGKDVLREARRFYAGPILVLSARDREAEKIAALDLGADDYVEK
                     PFAIGELLARLRAALRRHAHNPSATTRIEVDDLVVDLEKRRVVKNGAAIKLTPREYEV
                     LSVLARHAGCVVTHGQILTAVWGPAHKDDTQYLRVFIGQLRSKIENDPNAPTVIKTES
                     GIGYRFGE"
     gene            complement(3019..5739)
                     /locus_tag="VKU03_13980"
     CDS             complement(3019..5739)
                     /locus_tag="VKU03_13980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase KdpD"
                     /protein_id="HLJ72424.1"
                     /translation="MAQHDRDLDRRPDPDALLARAGQEKRGRLWVFLGAAPGVGKTYA
                     MLQRARGLKKAGVDIVVGLVETHGRAETAALLEGLEVLRRRNIAYRGHAIEEFDLDAA
                     LARKPQLMIVDELAHSNAPESRHPKRWQDVEELLDAGIDVWTALNVQHIESLADVVAR
                     IAGVAVRETVPDTVLRDAADIVLVDLTSDELIQRLKEGKVYLPETARRARENFFTPGN
                     LTALRELALRRAAARVDDQMVDYLKQNAIQGPWETAERLLVCIGPDALSETVVRAAAR
                     LATGLNASWVATTVQSPGREDTDPARIKRIDDALRLAERLGGETERLVGADLPAEIFA
                     YAQRENITQIVVGRSQAGWLARLRGRSLSSALVRRAQNINVHIITGERTLPAAPWRPA
                     LGRLSDLPIVIAAPAVSVAVAVAIGHALELWLRLPNLSMIFLAAVLLCAIRLGIRSAI
                     VAAILSFLAYDFFFVAPRYELTIAEPQEFFALVIFLIVAVLTGAIAGRARDQARSAQA
                     HAKTTQSLYELSRKLSGAASLDDILLAATVYAHKTLNARCVALLLPEEEGELDLLSSW
                     PPIDALDPGETSAAKWAFDKAEAAGWRTGTLPNVRFQFRPLATTRGAVGVCGVEPSDP
                     AAAFSPEQERVLTLILEQTAIAVDRSLLVRESVKTAALEENERLRDTLLASLSHDLRT
                     PLASITGAVTTLRQFGEKIASEQRADLLASIEEEAARMSRFVANLLDMSRIESGAINP
                     KRDYIAIADVVRAAVERSRKAFPDRPTTVNLARDLPFIRGDSHLLGQVLFNLLDNANK
                     YGGESGAIIHARRENDEIVVSVTDEGPGIKPADLERVFDKFYRGGRADGRKAGTGLGL
                     SICRGLVEAMGGKVHAESPAARRRGTRIVMRFPAVERPRRTEQPEWPNKS"
     gene            complement(5757..6386)
                     /gene="kdpC"
                     /locus_tag="VKU03_13985"
     CDS             complement(5757..6386)
                     /gene="kdpC"
                     /locus_tag="VKU03_13985"
                     /EC_number="7.2.2.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043829.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008556 - P-type potassium transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="potassium-transporting ATPase subunit KdpC"
                     /protein_id="HLJ72425.1"
                     /translation="MLNQIRPAIVMLALFSALTGLAYPLAVAGIAQMVAPSQANGGLL
                     RLKGKVVGSFLIGQNFASERYFHGRPSATQAQDPAHPDDPSKTIDAPDNAANSAGSNL
                     GPTSQKLVERVKADVAAQRAAGVTGPIPADSVTSSASGLDPDISPQYALDQVAAVAKA
                     RHLPAERVRALVQAHIEDRTVGIFGEPRVNVLLLNIALDELSSSSSSSS"
BASE COUNT         1165 a         2116 c         2136 g         1078 t
ORIGIN      
        1 agagcggaat aggatgtcgg cgcaaccggc atcgaggcgt atcgaggaac gtacgcggcg
       61 gcggtcgcgg cgactggcgc tttgctcacc cgatccagca tgcccaattg acccgccgac
      121 atgggttgtc cgatcacctg cgggcttcgc cacatgaacg gctggaaatt gtcggcgatg
      181 ggcggggagt tggccagggc tgctgttccg cctgaaacgg cgattgcgcc ggtcaaaagg
      241 actgtcttca acatgggtat ctccttgtcg aaccgatttt gctcagatcc ggccgtcgcg
      301 atgcgatcgc cgtcgagcgg tcttcggacg ttagcagccg attcttatgg cagagtgagc
      361 atatcgagcc gcgctgtgga aatgcgcgcc cgagcgcaga ggccggaacg atcggatcga
      421 ttggcgagcg tcaattcgaa cccatatttg cgagcgacgc tttgagcgat cgccagcccg
      481 aggccggagc cttccacgtc cgacggcgcc gcgcgataga accgttcgaa gacgcgaggc
      541 aattccgcat ccggaatgcc cgggcccgta tcgagaattt cgactgtcgc gagccccttc
      601 tgcgcaaaga cgcgtacgtc aacccggccc ttcgcgcccg tatagcggat cgcgttctga
      661 agcaggttcg cgaccaaaag cttcaggtct tcgcgcgagg ccagcagcgc cccggtcggg
      721 ctcgcgtcga gactgagatc gacgcctttg gcgtcggcga gcggcaggct ctccgcgacg
      781 caagcgctga cgaggtcggt cagatcgacg cgttcgtccc gccgcacggc ctcggacgcg
      841 tcgagtcgcg ccagcttgag catttgatcg accaaagcgg aggcgcgatc caatccgcgc
      901 ttcatctcgt cgaacgcctc gggatcgccc ccggcggtct cggccgccaa attgtcgatc
      961 tggatgcgta aagccgtcag cggcgtccgc agttcatgcg cggcgtcgga gacgaagcgc
     1021 cgctggcgaa ggaccatccg tcccaaccgg gtcgtcaagt cattcatcgc gaccagcacc
     1081 ggccgcaatt cgctcggcgc gtgatcgaga ggaatcggct ccgtcgattc gggcttccgc
     1141 gacgcgatcg cctcggcgac gatggtcaat ggccgcaaga cgcggccaag cgcgaagctg
     1201 aggaccaacc agcccaaagg aattgcaatc aggatcggca gaccggcttc gagccccgcc
     1261 gttgcggcga tttcgtcgcg caccaccatt ttctggccga cctggacgat ccgtcgcgag
     1321 tcgcgcgcca gaaacacgcg ccacgactcg ccgcctgcct gaatcgtacc aagcccagac
     1381 cgtccgaact gcggcagcag accgccaaag gaggtgagca gtcggccgtc cgggacgctc
     1441 cagacattaa cgaccaggcg gtcttcttcg tcctggtcga cagaaggcgt aaaggtttcg
     1501 ccgaccgagc cgccggcgtt gagggcgatc tggcgcaatt ggccgtcgag gaattccgcc
     1561 gcctgctggc gtcctatttt ataggaaacg ccaaaagcga tcgcgccgat cgccgcaagc
     1621 agaaccgtcg tccagatgag aaccgcccgc ctgagagaac tcacgatcca ttcctcgaca
     1681 cgacccagcc ggcccctcta atgttgcgga tgatattttt gccgaatttc ttcctcaccg
     1741 aatggatcag cacgtcgacg gcgttgcttt cgacctcctc gccccatcca taaagacgat
     1801 cttcgagttg agcgcgcgac aggatggcgc cgggcctctc catgagggcg cgcatcagcg
     1861 cgaactcccg ggccggcaag acgacgccgt tctcgccgcg gctgagcgta tgctcggcga
     1921 gattgaggca aagatcgcct gcatgcagaa gcgattcggc cgccccgctg cgccgccgcg
     1981 acacggcgcg aatgcgcgcg agaagctcct cggtcgcgaa cggcttcacg acataatcat
     2041 ccgcgccgag atcgaggccg cgaaccctat cctcgatctc gtctcgcgcc gtgacgatga
     2101 cgacgggagt gcgaacgccc gccgcgcgca tgcgccgcag cacttcaagc ccgccgatcc
     2161 cgggcaggcc caaatcgagg aggatcatat cgtgctcgcc ggaggcggcg gcctcggcgc
     2221 cctccgcgcc gtcgcgaacc caatcgaccg acgcgccttc gcgcccgagg ctgcggcgca
     2281 ggccgcgccc aatcatcgga tcgtcctcga tcaacaagat tcgcaacggc gtcctctccg
     2341 agacgaagcc gatcgcctat tcgccgaaac gataaccgat ccccgactcg gtcttaataa
     2401 ccgtcggggc gttgggatcg ttctctatct tggagcgcaa ctgcccgata aaaacccgga
     2461 gatattgcgt atcgtcctta tgcgccggtc cccagacggc ggtcaggatt tgcccatggg
     2521 tcaccacgca gccagcgtgg cgcgccagca cgctgagcac ctcgtattcg cgcggcgtca
     2581 gcttgatcgc cgcgccgttt ttgacgacgc gccgcttctc caggtcgacg accagatcgt
     2641 cgacttcgat cctcgtcgtt gcgctcggat tgtgggcgtg gcggcgcaag gcggcgcgga
     2701 gccgcgccag caattcgcca atcgcgaaag gcttctcgac ataatcgtcc gcgccgagat
     2761 cgagcgcggc gattttttcc gcctcgcggt cgcgcgctga aagcacgaga attggcccag
     2821 cgtaaaagcg ccgcgcttcg cgcaacacat ccttgccgtc catgtcggga aggccgagat
     2881 cgagcacgac gatgtcgggg gcggcgacgg cgatcagccg caaggcctcg cgtccgctcg
     2941 ccgctttcag cgcctcgtag ccggcggcgt tgagcgccgg gccgaggaag cgttggattt
     3001 gcggctcgtc gtcgacgatc aggacttgtt gggccattcc ggctgctccg tgcgccgcgg
     3061 gcgctccaca gcgggaaagc gcatgacgat gcgcgtcccg cgccgccgcg ccgcaggact
     3121 ctcggcgtgg accttcccgc ccatcgcctc gaccaggccg cggcagatcg aaagcccaag
     3181 accggtgccg gccttgcggc cgtcggcgcg gccgccgcgg taaaatttgt cgaaaacgcg
     3241 ctcaagatcg gcgggcttga tccccggccc ctcgtcggtg acgctgacga cgatctcgtc
     3301 gttttcccgc cgcgcatgaa taatcgcgcc ggattcgccg ccatatttgt tcgcgttgtc
     3361 gaggaggttg aagagaactt gcccgagcag atgcgaatcg ccgcgaatga aaggcaggtc
     3421 gcgggcgaga ttgaccgtcg tcggccggtc cgggaaagct ttgcgcgagc gctcgacggc
     3481 ggcgcgcacg acatcggcga tggcgatata gtcgcgcttc ggattgatcg cgcccgattc
     3541 gatgcgcgac atgtcgagga gattggcgac gaagcgcgac atccgcgccg cctcctcttc
     3601 gatcgaggcg agcaggtcgg cgcgctgctc ggaggcgatc ttttcgccga attgcctgag
     3661 cgtcgtcacc gcgccggtga tcgaggccaa aggcgtgcgc aaatcatgcg ataaggatgc
     3721 gagcaaagta tcgcgcagcc tttcgttctc ttccagcgcc gcggttttga ccgactcgcg
     3781 aaccagcagc gagcggtcga ccgcgatggc ggtttgctcc aggatcaagg tcaggacgcg
     3841 ctcctgctcc ggcgaaaaag ccgccgccgg atcgctcggc tcgacgccgc agacgccgac
     3901 ggcgccgcgc gtcgtcgcca ggggacggaa ctggaagcgc acattcggca aagtgccggt
     3961 ccgccagccg gccgcctcgg ctttgtcgaa agcccatttc gccgcgctcg tctcgccggg
     4021 atcgagcgcg tcgatcggcg gccaagacga caggagatcg agctcgcctt cctcctcggg
     4081 caaaagcagc gcgacgcagc gcgcgttcaa cgtcttatgc gcatagacgg tcgcggcgag
     4141 caggatatcg tcaagcgagg cggcgccgga gagcttgcgc gaaagctcgt agagcgattg
     4201 ggtcgtcttg gcgtgggcct gcgcgctgcg cgcctgatcg cgcgcccgtc ccgcgatcgc
     4261 gccggtcaag acggcgacga tgagaaagat caccagcgcg aagaattctt gcggctcggc
     4321 gatggtgagt tcgtagcgcg gcgcgacgaa gaagaaatcg taggcgagga aggagagaat
     4381 cgccgcgacg atcgccgagc ggatgccgag gcggatcgcg cagagaagga ctgcggcgag
     4441 gaaaatcatc gagaggttgg gcaggcgcag ccacagttcg agcgcatggc cgatcgcgac
     4501 ggcgacggcg accgagaccg cgggcgcggc gataacgatg ggtaaatcgc tcaatcggcc
     4561 tagcgccggg cgccaagggg cggcgggcaa ggtccgctcg ccggtgatga tatggacgtt
     4621 gatgttctga gcgcgccgga cgagggcgct gctcaaggag cggccgcgca gcctcgccag
     4681 ccagcccgcc tgcgagcgcc cgacgacgat ctgggtgatg ttctcgcgct gcgcataggc
     4741 gaatatttcg gccggcagat cggcgccgac cagccgctcg gtctcgccgc cgagccgctc
     4801 ggcgaggcgc aaagcgtcgt cgatccgctt gatcctggct ggatcggtgt cttcgcgccc
     4861 cggcgattgg accgtcgttg cgacccagct cgcgttgagg ccggtcgcca gacgggctgc
     4921 cgcgcgcacg acggtttccg acaaagcgtc cgggccgata cagaccaaaa gccgctccgc
     4981 cgtctcccag ggcccttgaa tggcgttctg tttgagatag tcgaccatct ggtcgtcgac
     5041 gcgcgccgcg gcgcgccgca gcgccaattc gcgtagggcg gtcagattgc ccggcgtgaa
     5101 gaaattttcc cgggcgcgcc gcgccgtctc cggcagatag accttgcctt ccttgagccg
     5161 ctggatcagt tcgtccgagg tgagatcgac aagaacgata tccgccgcat ccctcagcac
     5221 cgtgtcgggg acggtctcgc gcaccgcgac gccggcgatc cgcgcgacca cgtcggcaag
     5281 gctctcgatg tgctggacgt tgagcgcagt ccagacgtcg atgccggcgt cgagcaattc
     5341 ctcgacatcc tgccaacgct tgggatgccg gctttccggc gcgttgctgt gggcgagttc
     5401 gtcgacaatc atgagctgcg gcttgcgcgc cagcgccgcg tcgagatcga attcctcgat
     5461 cgcatggccg cgatacgcga tgttccgccg tcgcaggact tcgagccctt cgagcagggc
     5521 cgcggtctcg gcgcggccat gggtttcgac gaggccgacg acaatatcga cgccggcctt
     5581 cttaaggccg cgggcgcgct gcagcatcgc ataggtcttg ccgacgcccg gcgcggcgcc
     5641 aagaaagacc cagagccggc cgcgcttctc ctgcccagcg cgcgcaagca gcgcgtccgg
     5701 gtccggccgc cggtcgagat cgcggtcgtg ctgcgccaaa ggctccgtcc gtctcgtcac
     5761 gatgacgatg atgatgatga tgatagctca tccagcgcga tattgagcag gaggacattg
     5821 acccgcggct cgccgaatat gccgaccgtc cggtcctcga tatgcgcctg gactaaggcg
     5881 cgaaccctct cggcgggtag atggcgcgcc ttcgccacgg ccgcgacctg gtcgagggcg
     5941 tattgcggcg aaatgtcggg gtcgaggccg gaggccgaac tcgtcaccga atcggctgga
     6001 atcgggccgg tcacgcccgc tgcgcgctgc gcggcgacat ccgctttgac ccgttcgacg
     6061 agcttctgcg aggtcgggcc gagattggag ccggcggaat tggccgcgtt gtcgggcgcg
     6121 tcgatcgtct tgctcggatc gtcgggatgc gcgggatctt gcgcctgcgt cgccgacggg
     6181 cggccatgga aatatctctc cgaggcgaag ttttggccga tcaggaatga gccgacgact
     6241 ttgcccttca acctgagaag gccgccattg gcctggctcg gcgcgaccat ttgcgcaatg
     6301 ccggcgacgg cgaggggata ggccaaaccg gtcaacgccg aaaacaacgc gagcatgacg
     6361 atcgcggggc ggatttgatt caacattgtc cgttcctcac gccaaatgaa gcgccgagac
     6421 cagaagatcg atcagccaga tgccgatgaa cggcgcggcg aggccgccga gcccatagac
     6481 gagcagattg cggcg
//
LOCUS       DATGJW010000464         5300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1228173, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000464 DATGJW010000000
VERSION     DATGJW010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5300
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1228173"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..1028
                     /gene="lexA"
                     /locus_tag="VKU03_13990"
     CDS             333..1028
                     /gene="lexA"
                     /locus_tag="VKU03_13990"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HLJ72426.1"
                     /translation="MLTKKQNELLRFIHERLQEHGVPPSFDEMKEALDLRSKSGIHRL
                     ILALEERNFIRRLPNRARAIEVLRLPESATVATPRGGKFSPSVIEGHLGRARAGRGGA
                     HPAPAMIPVMGRIAAGTPISAIQTRTASIPMPPEFLGGGEHFALEVRGDSMIEAGIFE
                     NDTVIIRKQDTAETGDIVVALIDEEEATLKRLRRRGASIALEAANPAYETRIFGPDRV
                     RIQGRLVSLLRKY"
     gene            complement(1517..2527)
                     /gene="nagZ"
                     /locus_tag="VKU03_13995"
     CDS             complement(1517..2527)
                     /gene="nagZ"
                     /locus_tag="VKU03_13995"
                     /EC_number="3.2.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019903899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-N-acetylhexosaminidase"
                     /protein_id="HLJ72427.1"
                     /translation="MIRAFICGCAGPALSEEERLFIAEAQPWGLILFKRNVVEPAQMR
                     RLTQSFRALVGRPDAPVLIDQEGGRVQRMAPPHWPAYPSAASFETSGKDRSAALSAAR
                     LAARLIAHDLRDVGVTIDCAPVLDLSFKDAHSVIGSRAFSDDPARVAEFGRAVADGLM
                     AGAVAPVVKHMPGHGRAMVDSHLDLPIVEASRADLAAADFAPFVALKDLPIAMTAHVV
                     YRAIDPERPATTSKTIIETIIRGAIGFEGLLLTDDLSMKALGGSFEARTQAAFAAGVD
                     IALHCNGDLAEARAVAKAAPTLAGRSLRRAEAALATTAATAQPFDFRAARAELAAFAP
                     AA"
     gene            complement(2543..4474)
                     /locus_tag="VKU03_14000"
     CDS             complement(2543..4474)
                     /locus_tag="VKU03_14000"
                     /inference="COORDINATES: protein motif:HMM:NF016895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SPOR domain-containing protein"
                     /protein_id="HLJ72428.1"
                     /translation="MSETARRATPSVNIDEFERRLRATGSLGAAQDDPLAELARLLGA
                     EDPPARSGEIPDVKPAAPPPEPEASPAAPLLRPAPRIDGAYGQVVNVDFNAEPAASPS
                     EPPPLLAEPPPYAEHPAAPPPLLRAPVGGSRGEEPARRKVWPVAALVVIGVAGGAGAW
                     AYRGGVPGLVAKAPPLIMAAAGPTKVQPPSQATVSSPNDIASLIAKDEQNRAGPGKIV
                     SSEEQPVDLNERAKVQAAAEARAAAPPPSPPTIAAAPMVVVAAPSSASSAAPAAPPPA
                     SAAAASNSPPPSSLSPQPPAPSASPPAAQKPALAATPASAPHAAAAAPAPPPAPAAAP
                     SAPPQAAARSVALAAAAPSPVAPLDSTPIAPGAGTVGPTGAPPAPPTPFPEPKRVKTV
                     SVRPDGSIIAPDQPEQPVQPLEPPNLPANPPTPVPRPAIDASAAEPATPKLDLPTKLS
                     GKTTTRVAIPKIDTTAATTGQSPEAPLQIAPTTRESAASKKREAAHATQAEAAPPAAA
                     PAPAQAQESSGQSLFAALSDAAHRIVPGQENGTAGAPVRTASASPAAPASAAEGGGDY
                     AVQLAAPRSEAEARSIASRLESKYASELGGMQPVIRKAELKGKTIYRVRIAKLSHAAA
                     ISLCEKLKAAGGACFLARE"
     gene            complement(4519..>5300)
                     /locus_tag="VKU03_14005"
     CDS             complement(4519..>5300)
                     /locus_tag="VKU03_14005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265464.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DALR anticodon-binding domain-containing
                     protein"
                     /protein_id="HLJ72429.1"
                     /translation="DGGYTYFAADIAYHKDKVDRGFHTLIDVWGADHGGYVKRMQAAV
                     AAVSGGRATLDVRLCQLVRLMRAGEPVKMSKRAGDFVTLREVVDEVGVDAVRFMMLIR
                     RNDAPLDFDLAKVIEQSQDNPVFYVQYAHARGCSVLRQARAAFPDFDASPAVLARADL
                     ALLGDESERALLRLIAQYPRIVDQAAAAREPHRIAFFLHDLATGFHAHWNRGKDMPHL
                     RFVNERNRELTNARIALALSVTVVLSSGLALLGVGAPEEMR"
BASE COUNT          883 a         1781 c         1843 g          793 t
ORIGIN      
        1 acttttcggt caggcaagcc gacgggtcat acggattcgg ccatcaagaa gcagcggaag
       61 gacatgtgat catccggtat cctgcgcagc atagggcggc gacccttgaa gcgacgcgac
      121 gaaacggcgt cgtctatttc ggcaacgaac cgacaaattg cccctaaaac gcgccggccc
      181 atcgaagctg gcgcgatcct ttgcggattc cgcttgccag gaacacagca tgaacgtata
      241 agtccgttca cgatttgttt aagcagcgcc gcgagatttg gcgcgaaggc gagcgtaagc
      301 gattcgccgg ccggcaaaag gctgaaacgg acatgctgac caagaaacaa aacgaactgc
      361 tgcgcttcat tcacgagcgc ctgcaagaac atggggtgcc gccgtctttc gacgaaatga
      421 aggaggcgct cgatctgcgc tccaaatcgg gcatccatcg gctgatcctc gcgctcgaag
      481 agcgcaattt catccgccgc ctgccgaatc gcgcgcgcgc catagaggtg ctccgcctgc
      541 ccgaatcggc gacggtcgcg acgccgcgcg gcggcaagtt ctcgccgagc gtgatcgagg
      601 gccatctcgg ccgcgcccgc gccggccgcg gcggcgccca tcccgcgccg gcgatgatcc
      661 cggtgatggg ccggatcgcc gcaggcacgc cgatctcggc gatccaaacc cgcaccgcct
      721 cgattcccat gccgcccgag ttcttaggcg gcggcgagca tttcgcgttg gaagtgcgcg
      781 gcgattcgat gatcgaagcc ggcattttcg agaacgacac cgtcatcatc cgcaagcagg
      841 acacggccga gaccggcgac atcgtcgtcg cgctgatcga cgaagaggag gcgacgctga
      901 agcgccttcg ccgccgcggc gcctcgatcg cgcttgaagc cgccaatccg gcctatgaga
      961 cgcggatttt cgggccggac cgggtgcgca tccaaggccg gctggtgagc ttgctgagga
     1021 agtactgaag gcggtttcgt cccttcttcc ccgcaagcgg ggagaaggcg gcgcgaagcg
     1081 ccggatgggg ctgcgccgga ctcgaaaagt acgcactctt cgcaaggcaa atcggcggac
     1141 tcagcgcagc cccttgcacc cggccgtgct gcgcacggcc gcgcagcgtc cgcttcacgg
     1201 ggagaaggag aagggagcgc gctccctccc ggatggcgca gcgccaaaaa caggaggccg
     1261 gctggaaacg gctacgagga ggccactccc gccgtttcca agccggccga ccccacggac
     1321 ccaggagatg cggcggacct gagcactccg tagggtattc tttgcggggt ctcgctcgcc
     1381 ccctgacgcg ccaacgttac cgcgatggtt cgcgaaagca acggtttgat tgttcccgac
     1441 gttggaaacc ttttatttac ctaaagaaag tcggttaagc ggcaaattac agcgtggtta
     1501 accgtgacgc ggcaattcac gccgccggcg cgaaagccgc gagctcggcg cgggcggcgc
     1561 gaaagtcgaa aggctgagcg gtcgctgcgg tcgtcgccag cgccgcctcg gcgcggcgca
     1621 acgagcgccc ggcgagagtc ggcgccgctt tggcgaccgc cctcgcctca gcaagatcgc
     1681 cgttgcaatg cagggcgatg tcgacgccgg cggcgaaagc cgcttgcgtg cgcgcctcga
     1741 acgagccgcc cagcgccttc atcgacaaat cgtcagtgag cagcagcccc tcaaagccga
     1801 tcgcgccgcg gatgatcgtc tcgattatgg ttttcgaagt cgtcgccggc cgctcgggat
     1861 cgatcgcgcg atagacgaca tgggcggtca tcgcgatcgg caaatctttc agggcgacga
     1921 agggcgcaaa atccgccgcc gcgaggtccg cccggctcgc ttcgacgatc ggcaaatcga
     1981 gatggctgtc gaccatggcg cggccgtgcc ctggcatgtg tttgacgacc ggcgcgaccg
     2041 cgccggccat gagcccgtcg gcgacggcgc ggccgaattc ggcgacgcgg gccggatcgt
     2101 ccgaaaaagc gcgcgagccg atcaccgaat gcgcatcttt aaaggaaagg tcgagcaccg
     2161 gcgcgcaatc gattgttacg cctacatcgc gcaggtcatg cgcgatgagg cgggcagcca
     2221 gccgggcggc gctgagcgcc gcgctgcggt ctttgcctga ggtctcgaag ctcgccgcgc
     2281 tcggataggc cggccagtgc ggcggcgcca tgcgctgaac ccggccgcct tcctggtcga
     2341 tcagcaccgg cgcatcagga cgcccgacga gcgcgcgaaa cgattgcgtc agccgccgca
     2401 tctgcgccgg ctcgacgacg ttgcgcttaa acaggatcag gccccaaggc tgcgcctcgg
     2461 cgatgaaaag acgctcttct tcgctcagcg ccgggccggc gcagccgcag ataaacgccc
     2521 tgatcatctc cgccgccttc gcctattcgc gcgccaggaa gcacgcgccg cccgccgcct
     2581 tcagcttttc gcataggctg atggccgcgg cgtgtgacag cttggcgatc cgcacccgat
     2641 agatcgtctt gcctttcaac tcggctttgc ggatcaccgg ctgcatgccg ccaagttcgc
     2701 tcgcatattt cgactcaagg cggctcgcaa tgctgcgcgc ctcggcttcg gagcgcggcg
     2761 cggcgagttg aacggcatag tcgccgcccc cttccgccgc cgacgcgggc gcggccggcg
     2821 aagcggaagc ggtccgcacc ggcgcgccgg cggtcccgtt ctcctggccg ggcacaatgc
     2881 gatgcgcggc gtcgctcaac gcggcgaaga gcgattgccc ggaggattcc tgggcttggg
     2941 ccggcgccgg cgcggcggcc ggcggcgcgg cctcggcctg cgtggcatgc gccgcctcgc
     3001 gctttttcga ggctgcggat tcccgcgtcg tcggggcgat ttgcagcggc gcttccggcg
     3061 actggccggt cgtcgcggcg gtcgtatcga tcttcgggat cgccaccctg gtcgtggtct
     3121 tgccggaaag cttggtcggc aggtcgagct tcggcgtcgc cggctcagca gcggacgcgt
     3181 cgatcgccgg ccgcggcacg ggcgtcggcg gattggccgg caggttcggc ggctcgagcg
     3241 gctgaacggg ctgttccggc tgatccggcg cgatgatcga gccgtccggc cgcaccgata
     3301 ccgtcttcac ccgcttcggc tccgggaaag gcgtcggcgg cgccggcggc gccccggtcg
     3361 ggccgaccgt tccggcgccc ggcgcaatcg gggttgaatc gagcggcgcg accggcgacg
     3421 gcgcggcggc ggcaagggcg accgaacgcg ccgctgcctg gggcggcgcg gaaggcgcgg
     3481 cggctggcgc tggcggggga gccggcgcgg ccgcggctgc gtgcggcgcg ctcgcaggcg
     3541 tagcggccag ggcgggcttc tgcgcagccg gcggcgatgc agaaggcgcc ggcggttgag
     3601 gcgaaagcga agagggagga ggcgagttcg aagcagcggc cgcgctggcg ggcggcggcg
     3661 cggcgggagc ggccgacgac gccgatgacg gcgcggcgac gacgaccatc ggcgcggcgg
     3721 cgatggtcgg cggactgggc ggcggcgcgg cggcgcgcgc ttccgcggcg gcctggacct
     3781 tggcccgctc gttgaggtcg acgggttgct cttcgctcga gacgattttt ccagggcccg
     3841 cccggttttg ctcgtccttg gcgatcagcg aggcgatatc attcggcgac gaaacggtcg
     3901 cctgcgacgg cggctgcacc ttcgtcggcc cggcggccgc catgatcaag ggcggcgctt
     3961 tggcgacgag gcccgggacg ccgccgcgat aggcccaggc gccggcgccg ccggcgacgc
     4021 cgatcacgac cagcgcggcg accggccaaa ccttgcgccg cgccggctct tcgccgcggc
     4081 ttccgccgac cggcgcgcgc agcaagggcg gcggcgccgc cggatgctcg gcatagggcg
     4141 gcggctcggc cagaagcggc ggcggctcag aggggctcgc ggcgggttcg gcgttgaaat
     4201 cgacattgac gacttgaccg tacgcgccgt cgatgcgcgg cgcgggacga agcagcggcg
     4261 cggccggcga cgcttcaggc tcgggcggcg gcgcagccgg cttgacgtcc gggatctcgc
     4321 cggaacgggc cggcggatcc tcggcgccga gcaggcgagc gagttcggcg agcggatcgt
     4381 cctgagccgc gcccaaggac ccggtcgccc gcagccgccg ttcgaattca tcgatattga
     4441 ccgacggggt cgcgcgacgc gcggtctcgc tcatccaaaa tcctcttcgt tacgcccgcc
     4501 aaccccgccc gcttggcttc agcgcatctc ttccggcgcg ccgaccccaa gcagcgcaag
     4561 acccgacgac aggaccaccg tcacagagag cgccaacgct atgcgcgcat tggttaattc
     4621 tctgttgcgt tcattaacga aacgcaaatg cggcatatct ttgcctcggt tccagtgggc
     4681 gtgaaagccg gtcgccagat catgcagaaa aaaggcgatt cgatgaggct cgcgcgccgc
     4741 cgccgcttga tcgacgatgc gcggatattg cgcgatcagg cgcaaaagcg cgcgctcgct
     4801 ctcgtcgccc agcaaagcaa gatcggcgcg cgccaaaacg gcgggcgacg cgtcgaaatc
     4861 cggaaatgcc gcccgcgcct gacgcaagac gctgcagccg cgcgcatggg cgtattgtac
     4921 ataaaagacc ggattgtcct gactctgctc gatgaccttg gcgaggtcga aatcgagcgg
     4981 cgcgtcgttg cggcggatca gcatcatgaa gcggacggcg tcgacgccga cctcgtcgac
     5041 gacttcccgc aaagtgacga agtcccccgc ccgcttcgac attttgaccg gctcgccggc
     5101 ccgcatcagc cgaacgagct ggcaaaggcg cacatcgagc gtcgcccgcc cgccggaaac
     5161 cgccgcgacc gccgcctgca tgcgcttgac atagccgcca tggtcggcgc cccagacgtc
     5221 gatcagggta tggaagccgc ggtccacctt gtccttgtga taggcgatat cggcggcgaa
     5281 ataggtatag ccgccgtcca
//
LOCUS       DATGJW010000465         4415 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_818491, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000465 DATGJW010000000
VERSION     DATGJW010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4415)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4415)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4415
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_818491"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(134..1357)
                     /locus_tag="VKU03_14010"
     CDS             complement(134..1357)
                     /locus_tag="VKU03_14010"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011439891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ72430.1"
                     /translation="MTSNVSRFPARNLHGDAAEERDPRRLRSRAKEAADVAARHAAAV
                     DRDARFPSEAFAAIREQKLLGLLIPAALGGEEASAADVAEVCYILGGACASTAMIFAM
                     HQVKVACLARHSRGVAWQENLQRRIAAEQLLMASSTTEGSNGGNVRSSEAPVRTQGQH
                     ISLERATSCISYGEHADGIVTTARRNEAAAGSDQVLVAFLKDDYSLERTQSWDTLGMR
                     GTCSVGFILRAKGVAEQVLADPYERIHSQTMVPFAHMFWSAAWAGLAAGAVQRARDFV
                     RAAARASGGKMPPAAAHLTRARLSLETLRGAVHSAIESFERRSRDSEGLDDLNAQTAL
                     NLLKVEASELAVATVLSAMRACGLSGYRNDTEFTLGRHLRDVLSSPIMINNDRILSNI
                     ATASLMSAVPATLRD"
     gene            1642..1896
                     /locus_tag="VKU03_14015"
     CDS             1642..1896
                     /locus_tag="VKU03_14015"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphopantetheine-binding protein"
                     /protein_id="HLJ72431.1"
                     /translation="MLDRPNLTNRIFEMVREATNETAPSTTIGLDDDLGRRGLTSMGM
                     VKLMMAAEIAFDIAIPDEDLHPDNFRSVRSIEALVTRLAK"
     gene            1911..2846
                     /locus_tag="VKU03_14020"
     CDS             1911..2846
                     /locus_tag="VKU03_14020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ72432.1"
                     /translation="MPTLDRAARRFLDVARASVTQSAGQASLDDMRRATEALAAFASS
                     ERRRDVATRDASLRSPAADLPIRVYSPRAAQAKISPGLIYLHGGGWISGSLDSHDGIC
                     RALADEGACRVVAVAYRLAPKHPFPAALEDGAHAIEAIAAEAEGFAIDPRRIGVAGDS
                     AGGNLAAALCGALRGGPPIALQLLLCPVLDALGRTPSRRALAHGYFLEERTMVRYFEH
                     YQAEGLAPDDPRVSPLRAADFSGLPPTRIHAAEYDPLKDEAALYAERLAEAGVDVRLT
                     VHQGMIHHFYGLGDVIPYAGAALKTIGAEIREALS"
     gene            complement(2857..>4415)
                     /locus_tag="VKU03_14025"
     CDS             complement(2857..>4415)
                     /locus_tag="VKU03_14025"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015929924.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="long-chain-acyl-CoA synthetase"
                     /protein_id="HLJ72433.1"
                     /translation="AGDPVCLLMPNRPDYVAIWLGVTQIGGVVALLNANLSGAALAHC
                     VDAAAPRHIIVAGELAERFAEAAPHLKTRPRLWLHGDADLAGPRLDEMIERLDGGPLN
                     PSERRAITLADRALLIYTSGTTGLPKAAHVSHHRIMMWSRWFAAVLDVGANDRMYDCL
                     PLYHSVGGVVAIGSALVNGGSAVVAERFSARQFWDDVARWECTLFQYIGELCRYLLAA
                     PPHRAEKGHRLRAACGNGLSADVWEAFQARFAVGRIVEFYAATEANFSLTNVEGKVGA
                     IGRLPGFLAAQSRIALVRFDVAKGEPARGPDGFCIRAARGEAGEAIGRISAGAGDLAG
                     RFEGYTNAAETEKKILRNVFAPGDAWLRSGDLMRMDGEGFYYFVDRVGDTFRWKGENV
                     STLEVANALRTCPGVADAAVYGVVVPGAEGRAGMALVASDGKLDLDRLRRAMAEALPL
                     YARPLFLRIRPEIEITATFKHQKQALARDGFDPARIADPLYVFDREKDAYVALDQEVF
                     AAIASGAMRL"
BASE COUNT          746 a         1443 c         1461 g          765 t
ORIGIN      
        1 tcttgtggaa caatttgtcc gccagatgat cgaggggatc ggcgggctca ggggcgttct
       61 caagagagtc ggtgcggatg gttaggttca tcttacgacc ttcggtgatg ctgttcagct
      121 ctttctgttt ggctcagtcg cgcaaggttg cgggcacggc gctcatcagg ctcgcagttg
      181 cgatattgct gaggatgcgg tcattgttga tcatgatcgg cgacgacagc acgtccctca
      241 aatgccgccc caacgtgaat tccgtatcgt tgcggtagcc ggacaggccg caggcccgca
      301 ttgcgctcag gacggtcgca acggcgagtt ccgacgcttc gactttcaaa aggttcagcg
      361 cggtctgcgc attgagatcg tcaagcccct cgctgtcgcg gctgcggcgc tcgaaactct
      421 cgatggcgga atgaaccgcg ccgcgcaaag tctccaggct cagccgcgcc cgtgtcagat
      481 gcgccgcggc cggcggcatt ttgccgccgg aggcgcgcgc cgccgcgcgc acgaaatcgc
      541 gcgcccgttg caccgcgccg gccgcaaggc ccgcccaagc ggcgctccag aacatatgcg
      601 cgaacggaac catggtctgg ctatggatgc gctcgtaagg atcggcgaga acctgctcgg
      661 cgacgccctt cgcccgcagg atgaagccga cgctgcacgt gccgcgcatg ccgagcgtgt
      721 cccaggactg ggtgcgctcg agcgagtaat cgtccttgag aaaggcgacg agcacctggt
      781 ccgatcccgc agccgcctcg ttgcgcctgg cggtcgtgac gattccgtcg gcgtgttcgc
      841 cgtaggaaat gcaggatgtg gcgcgttcga gggaaatatg ctggccttgc gtgcgaaccg
      901 gcgcttcgct cgaacggaca ttgccgccat tcgatccttc ggtggtcgaa gacgccatca
      961 gcaattgctc ggcggcgatt ctgcgctgca gattctcttg ccaggcgacg ccgcgcgaat
     1021 gacgcgcgag gcaagcgacc ttgacctgat gcatggcgaa aatcatcgcc gtggaggcgc
     1081 aggcgccgcc gagaatgtag cagacctccg cgacatcggc ggcgctcgcc tcctcgccgc
     1141 ccaaagcggc aggaatgagc aatccgagca gcttctgctc gcggatagcg gcgaacgcct
     1201 cgctgggaaa gcgcgcgtcg cggtcgactg cggccgcgtg gcgcgccgcg acatcggccg
     1261 cttcctttgc tcggcttctc aaccggcgcg ggtcgcgctc ctcggccgca tcgccgtgca
     1321 gattgcgcgc cggaaagcgc gagacattgg aggtcatgat cgccgctctt tcgttctggg
     1381 cgccggcgca aatcgagacg cccgccccat tccccgaata ccgtcttgtt ttccggcaag
     1441 agctatgcca gtcgcggctt aacgatccat ccgggcgatc tgcgagattc cgcctgtatg
     1501 ccgttaaccc ggcatgaaac atgctgtgtt ctttttgcgc tcctgggggg cggaattgcc
     1561 ggaaattcta gaaatctccg acaatcgaaa cggcggaggc gagccgggcg acggcgtcgt
     1621 ctcgtaacgg gaaaaagcga gatgttggac cgtcccaatt tgaccaatcg tatatttgaa
     1681 atggtgcgag aggcgaccaa cgaaaccgcg ccctcgacga caatcggact cgacgacgat
     1741 ctcggtcgga gaggcctgac ctcgatgggc atggtcaaat tgatgatggc cgccgaaatc
     1801 gcgttcgaca tcgcgatacc ggacgaagat ctgcatcccg acaactttcg ctccgtccgc
     1861 tcgatcgaag cgctcgtcac gcgattggcg aaatgaatcg ctagacaggg atgccgactc
     1921 tcgaccgcgc cgcccggcgg ttcctcgacg ttgcgcgcgc gagcgtgacg caatcggccg
     1981 gccaggcttc gctggacgac atgcggcgcg caaccgaggc gcttgccgct ttcgcctcaa
     2041 gcgagcgccg gcgtgacgtg gcgacgcgcg acgcgtctct gcgcagtccc gccgccgacc
     2101 tgccgatccg ggtctattcg ccgcgcgcgg cgcaagcgaa gatttcgcct ggcctcatct
     2161 atcttcatgg cggcggctgg atttcgggca gtctcgactc gcatgacggg atttgccgcg
     2221 ccttggccga tgaaggcgct tgccgggtgg tcgccgtcgc ctatcgcttg gcgccgaagc
     2281 atccgtttcc ggcggcgctg gaggacggcg cccacgcgat cgaagcgatc gctgcggaag
     2341 cggaaggttt cgcgatcgat ccgcgccgca tcggcgtcgc cggcgattcc gctggcggca
     2401 atctcgccgc cgcgctgtgc ggcgccttgc ggggcggacc gccgatcgcc ttgcagctcc
     2461 tgctctgtcc ggtcctcgac gcgctcggcc gcacgccctc gcggcgcgcc ctggcgcacg
     2521 gctattttct cgaagagcgg acgatggttc gctatttcga gcattatcag gccgaaggat
     2581 tggcgccgga cgatccgcgc gtctcgccgc tccgcgcggc cgatttcagc ggcctgccgc
     2641 cgacgcgaat tcacgccgcc gaatacgatc ccctcaagga cgaagccgcg ctttatgcgg
     2701 agcggctcgc cgaagccggc gtcgacgtgc gcctgaccgt ccatcaaggc atgatccatc
     2761 acttctacgg attgggcgac gtcatccctt acgcgggcgc ggcgctcaag acgatcggcg
     2821 ccgagatccg cgaagcgctg tcataagcgc aagcggctat agccgcatcg cgccgctggc
     2881 gatcgcggcg aagacctctt gatcgagcgc gacataggcg tccttctcgc ggtcgaagac
     2941 ataaagcgga tcggcgatcc gcgccggatc gaagccgtcg cgcgccagcg cctgcttttg
     3001 atgcttgaat gtcgcggtga tttcgatttc gggccgaatg cgcaggaaga gaggccgcgc
     3061 gtaaagcggc agcgcttccg ccatggcgcg gcggaggcga tcgagatcga gctttccgtc
     3121 gctcgccacc agggccatgc cggcgcggcc ttcggcgccc gggacgacca cgccatagac
     3181 ggctgcgtcg gcgacgcccg gacaggtccg cagcgcgttg gcgacctcca gggtcgagac
     3241 gttttcgcct ttccagcgaa acgtgtcgcc gacccgatcg acaaaatagt agaagccctc
     3301 gccatccatg cgcatgaggt cgccggagcg aagccaggcg tcgcccggcg cgaacacgtt
     3361 tcggagaatc ttcttttcgg tctccgccgc gttcgtatag ccctcgaagc gcccggccag
     3421 atcgcccgcg ccggcgctga tcctgccaat cgcctccccg gcttcgccgc gagcggcccg
     3481 gatgcagaag ccgtcgggcc cgcgcgccgg ttcgcctttc gcgacgtcaa agcggaccag
     3541 cgcgatccgg ctttgcgcgg cgagaaagcc cggcagccgg ccgatcgcgc cgactttgcc
     3601 ttcgacattg gtcagcgaga aatttgcttc ggtcgcggcg tagaattcga ctatgcggcc
     3661 gacggcgaag cgcgcttgga aagcctccca gacgtcggcg ctcaggccgt tgccgcaggc
     3721 cgcgcgcagg cgatggcctt tttctgcgcg gtgcggcggc gcggcgagaa ggtaccggca
     3781 gagttcgccg atatattgga agagcgtgca ctcccagcgc gcgacgtcgt cccaaaactg
     3841 ccgggccgag aagcgctccg cgacgacggc cgaaccgcca ttgacgagcg ccgagccgat
     3901 cgccacgacg ccgccgacgc tgtgatagag cggcaggcaa tcgtacatgc ggtcattggc
     3961 gccgacatcg aggacggcgg cgaaccagcg gctccacatc atgatgcggt gatggctgac
     4021 atgcgccgcc ttcggcaggc cggttgtgcc tgacgtgtag atgaggagcg cgcgatcggc
     4081 gagcgtgatg gcgcgccgct ccgaaggatt cagcgggccg ccatcgaggc gctcgatcat
     4141 ttcgtcgagg cgcggtcctg caagatcggc gtcgccgtgg agccataggc gcggccttgt
     4201 cttcagatgc ggcgcggctt cggcgaagcg ttccgccaat tcgccggcga cgatgatgtg
     4261 gcggggcgcc gccgcatcga cgcaatgagc cagcgccgct ccgctcagat tggcgttgag
     4321 cagcgcgacg acgccgccga tctgggtgac gcccagccaa atcgcgacat agtccggccg
     4381 gttcggcatg agaaggcaaa ccgggtcgcc ggcgg
//
LOCUS       DATGJW010000466        13008 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1538828, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000466 DATGJW010000000
VERSION     DATGJW010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 13008)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 13008)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..13008
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1538828"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..284)
                     /gene="soxR"
                     /locus_tag="VKU03_14030"
     CDS             complement(<1..284)
                     /gene="soxR"
                     /locus_tag="VKU03_14030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011428862.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0051537 - 2 iron, 2 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensitive transcriptional activator SoxR"
                     /protein_id="HLJ72434.1"
                     /translation="METVLTIGETARRSGVAASALRFYEEKGLIASERAGSGRHRRFP
                     RSALRRIAFIVFAQKIGLSLEEIAAELAKLPAGRTPTRGDWEKLSGEWSKR"
     gene            370..1089
                     /locus_tag="VKU03_14035"
     CDS             370..1089
                     /locus_tag="VKU03_14035"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011428861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72435.1"
                     /translation="MRRENAFEDLRVAITGGTSGLGLALTRLMTRCGARVAFVARTVS
                     SVERVAQETGAHGIVGDVGRKDDIHPIALQILGNLGGLDVLINNASSLGPVPLALLSD
                     TECEELEQALAVNLLGVFRLTRALFGALAAAARDGRGALVVNISSDAAVTAYPGWGAY
                     GASKAALRHLTAIWDKEEKDDSVRFLSFDPGDMDTPLHALALPETDPATLKRPEDAAA
                     EIAEAIGASLRRAERFAGARP"
     gene            1086..2165
                     /locus_tag="VKU03_14040"
     CDS             1086..2165
                     /locus_tag="VKU03_14040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007537518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-adenosylmethionine:tRNA
                     ribosyltransferase-isomerase"
                     /protein_id="HLJ72436.1"
                     /translation="MIAARHFDRRGGKLLVVGAKGAMRRLPRTELASLFEAGDLIVAN
                     DAATLPASLKGTHRETGAPVEIRLAAWIRLGDPTGFVAIAFGAGDHRTRTEDRLAPPP
                     LAAGDRLALGPLQAIIERVLDHPRLLVLRFLGERAAIHAGLARHGRPIQYAHVPEPLA
                     LWDVWTRIAAAPAAFEAPSAGFALDWATLAAWRRRRIGFATLTHAAGISSTGDALLDK
                     RLPFDEPYQVPHSTAAAVAAAKARGGKVVAIGTSVVRALEAAADEAGVIRAGDGVARG
                     RIGQETRLRIVDALLTGAHQPGESHFELLKAFADEATLERMSAAFIANDFRGHEFGDS
                     VLIARARRSPEDRAMSALVPASLAE"
     gene            2276..3109
                     /locus_tag="VKU03_14045"
     CDS             2276..3109
                     /locus_tag="VKU03_14045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HLJ72437.1"
                     /translation="MQTLSIFQSLWAMERRHTDGLERSLEENVAMIAEAGFAGVSADW
                     RNRDFVKRLAGLLKAANMRAEGQCFPKTVDDLKPVLDIATEFGVHHLDIQPDVRPRRL
                     QDCVSLLEGWRRLAEDVDFPVFIETHRDRMTTDLYFTLDLLDCFPDLKLLADLSHFLV
                     GREFAWPVSDENHAMIHRILDNSWAFHGRVASREQVQIEISFPHHQMWVDLFLEWWEY
                     GFRSWRKRAGPNDDLAFTCELGPKPYAITGPDGNDTTDRWREALLMKEKVEQVWAKTE
                     A"
     gene            complement(3192..4229)
                     /locus_tag="VKU03_14050"
     CDS             complement(3192..4229)
                     /locus_tag="VKU03_14050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763145.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HLJ72438.1"
                     /translation="MNDIIKTDAVIVGAGPIGLFAVFELGLLDIKAHLIDILPKVGGQ
                     CAELYPEKPIYDIPGFAMVTGQGIVDNLLTQIKPFGPTFHLGEMVVGLDVLGEAGRPL
                     FRIATDADKVFEAKVVIIAAGGGSFQPKKPPIAGIDAYEGKSVHYAVRKIEQFRGRRV
                     LIVGGGDSALDWVLNLHPVAERVTLLHRRDAFRAAPHSVNAMRELAAAGKMDFILGQV
                     AGLKGEAGRLHAATIRGEDGSVFDHPADDMLPFFGLTMKLGPIADWGLNLHENLVPVD
                     TEKFETSAPGIFAIGDINTYPGKLKLILSGFHEAALAAQKVHRYVYPDKKLLFQYTTS
                     STSLQRKLGVA"
     gene            complement(4258..4578)
                     /locus_tag="VKU03_14055"
     CDS             complement(4258..4578)
                     /locus_tag="VKU03_14055"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020092346.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HLJ72439.1"
                     /translation="MIRITYNDSEGASRTVEAQEGSTVMETALRESVPGIEAECGGAC
                     ACATCHVYVAEEWMDVVGKPEQMEEDMLDFAFEVRPNSRLSCQIKVTPALDGLVVTTP
                     PRQG"
     gene            complement(4722..5135)
                     /locus_tag="VKU03_14060"
     CDS             complement(4722..5135)
                     /locus_tag="VKU03_14060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272593.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Hpt domain-containing protein"
                     /protein_id="HLJ72440.1"
                     /translation="MNDVAPILYKDWENAAWPSAAAVAAPSPPIDLVHLARQSLGDQD
                     LERELLAMFQQQAARLVGQMAAARGDDFKLRTALAHTLKGSALAVGANRVAEAAAQVE
                     AASAEGSGQVALAASLSSLAGAVSEAREAIARLLA"
     gene            5412..10994
                     /locus_tag="VKU03_14065"
     CDS             5412..10994
                     /locus_tag="VKU03_14065"
                     /inference="COORDINATES: protein motif:HMM:NF013600.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72441.1"
                     /translation="MAQQNKAQDAVAAAMSAIEEALNMSVADQAKEGKPAEPADEAKS
                     QAAKPLPNIAAAPAPSVASAAPERRAAAEPRAMPAPIRPPKAEAPKADAPSLTPPPLP
                     SAGEPPAAPPPPLPTTPPANDDRPSVGQLLLAFQSRPPSRAPVIAALAASVLWLLFCG
                     AFLAQHGDLFGGRSPVSREFWLQPQIALAALVVVGPVLFIFAIAALVRRSQELRGAAR
                     AISQVAMRLAEPETVASEQVATLSQAIRREVASMGDGIERALARASELETLVRGEVSN
                     LERSYSDNERRVRALIAELADQREAIVANGGKVRAAIAGAHQGVAADLDAVADRLNER
                     ILTAGQGLVASLGATAEGVGVALERAGADTIEKIGSHSAHLTQTLAAVGADVANRLAE
                     ATDRNAASLIERVADIDQRVKSTGEALTADLTVRGEDLVGRLDAAGARVSDAMIGHGE
                     SLVARVAQTSEAMLADFTARADDVAARLDNTGGRIAETILDRGEGLVSRLAETTDRMH
                     EVVAVRGEQFHARLAGAHDQLVAALSERADDAQRLFESTAANWAAEFDARHAALGAQL
                     EQHASDVNARFEETSQRTIAAIRDNSAEMHERLATTASDSIARMDEQTSRLADRFAAS
                     AGEAVAAIAIHSESVNETLNSQLANFEQTFSGRGAAAISALADQTGRLGEQLETLDRL
                     VGAAGEGFVERIGAHADRVTAHIDQQIGALDGAMAGRSAEIDRRLAEHSQKFMEVSGA
                     RLAEFETASVAYHADLDRALASHSERVDNTIREGIAGFESISGARHIDLATALGAHSA
                     RVDNAMREGLAGLETVSGARQADLNAALASHTSRVDATLRDGLAGFESVALAHHENLT
                     GALGAHSSRIDSTIRDGLAGFEAATAARQTELSEVLGSHAERIDGAIRGGLAGFETLA
                     ASHHGDLSEALNAHTGRVDSAMREGIAGFETASAAHHAELNSALATHSERVEHALREG
                     LVGFDAALSGRVGDMVDRVESQTDALVGRIGDKLSAIEETFTVHGPALDARLGARSAE
                     AIALFENNLKTLDDRTAVRSEHISKTMETLLDRVDNGLETRARALNDTLAQRTLEMAR
                     TMSLGGREVVSALDAKSKEIGEALQSRAATLTEALSGQAREINATLGGNLIALSGALG
                     ESVDRMQERVVAPLEGLSTTLGALVSGHADSIAATLGEHRKVIDETIGAHGAAIRRSL
                     ESGSAEIETGMAKAIAVLESAFAAHGGGLSKTIGEQIDDLRSIVEDKGGDFIARLGAR
                     GGDMSAELANAADRAAQAFDQRTTALINLLTRRSEDLLGALNAGSAASLQSLGALTAQ
                     IGQDAQNAAALLRTAADETAASASQAISGLSEKLRQEVEQSASQLRETLERSGGASVN
                     ALSGAGERLRNELLQVLDRLGATSSTLERVVESASSDLEAVRGGLADRVGEFERALGA
                     ISSQIAALGRASATTQKEAAGLADRLADHVNSLAGAAHDLASMQNTFDDAIARRRDSL
                     ANLIEEVASKSDAFENLMQTFTTTVEDSLNRALQRAKDVQTSIAQTTSGAARQITDQF
                     ETIRDHAGKERERTAAALEEAYEQATAQLSTIMNLANDRFKQTAASLRATTVEITREL
                     EATRQELQRGVLELPRETSEQAAAMRRVVGDQIKALNELAEIVARAGGTFDVAEPAAP
                     PIAAPPRALEAPRAEPARLQAEPAPQEPPRPRPAARSPAANPAPSAAARGQVGWLSDL
                     LSRASREESEPAVPAGAESRPSVEQFDGISLDIASLIDPAAAAEMWDRWRQGDTSAFS
                     RRLYTAQGQQTFDEIRRRYKADPQFRETVTKYVQEFERLLAKIGRDDRDGSHSREYLL
                     SDNGRVYTMLAHASGRLG"
     gene            11262..11735
                     /locus_tag="VKU03_14070"
     CDS             11262..11735
                     /locus_tag="VKU03_14070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015243097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HLJ72442.1"
                     /translation="MALRDLIPGGGGRDVATRRSDPFFALQRDMNRLFDDFFRGFDLT
                     PFSGDHGAGWPNIEVSETEKDVKVMAELPGLDEKDVQVDFANGVLAIKGEKKTETDDK
                     ERLFSERYYGRFERRIPVDDVDEDKVSATFKNGVLTVTMPKAAQSKVKRIAINGK"
     gene            12097..>13008
                     /locus_tag="VKU03_14075"
     CDS             12097..>13008
                     /locus_tag="VKU03_14075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017956947.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TauD/TfdA family dioxygenase"
                     /protein_id="HLJ72443.1"
                     /translation="MAAAGRFTLTAVSLELQEPSTRIRNAEPRERALTIHWSDGAAAD
                     FPYLFLRDNCPSGFHPQTQERLFDLLSIPDELKPRHVATDGRTLTIDWPGEPSHRTVL
                     DADWLADHRPGARPPDPANIVSVSWGGDFLPRLPRREAAALARDPAAMKDWLIALKRD
                     GLSVVTGLADDEEAGVEFGKTIGFLRRTNFGETFRVETKPDPNNLAYTSHALALHTDL
                     PNQEMPPGFQFLHCVRNDAAGGGSIFLDSFRIAERLRESDPKGFALLSTVPIPYRFHD
                     REYDIRIDRPLIGLDERGRLFDVRFSAH"
BASE COUNT         2209 a         4456 c         4300 g         2043 t
ORIGIN      
        1 cgtttcgacc attctccgga cagtttctcc caatcgccgc gagtcggcgt gcgcccggcc
       61 ggcagcttgg cgagctcggc ggcgatctcc tccaggctga ggccgatctt ctgcgcgaaa
      121 acgatgaagg cgatgcgccg caaggccgag cgcggaaatc ggcggtggcg gccggagccg
      181 gcccgctccg aggcgatgag gcccttttct tcgtagaagc gcaaagcgga ggcggcgacg
      241 ccgctgcgcc gcgccgtctc gccgatcgtc aggacggtct ccatgaaccc gtcgaagcac
      301 gaatccggct tgacttcaag ttcactttaa cttgtagtca agccgctcct tgaattgagg
      361 agctttttca tgcgcagaga aaacgcgttc gaagaccttc gcgtcgcgat caccggcggc
      421 acgtcggggc ttggcctggc gctgacgcgg ctcatgaccc gctgcggcgc gcgcgtcgcc
      481 ttcgtcgcgc gcaccgtttc gtccgtcgag cgcgtcgccc aagagaccgg cgcccatggg
      541 atcgtcggcg atgtcggccg caaagacgat attcatccga tcgccttaca gattttgggc
      601 aatctcggcg gcctcgacgt gctcatcaat aacgcttcga gcctcggccc ggtcccgctc
      661 gcgcttctgt cggacaccga atgcgaagag ctggagcagg cgctggcggt caatctcctc
      721 ggcgtcttcc ggctgacgcg cgctctgttc ggcgcgctcg ccgccgcggc gcgcgacggc
      781 cgcggcgcgc tggtcgtcaa catttcgagc gacgcggcgg tcacggccta tccgggctgg
      841 ggcgcctatg gcgcgagcaa ggcggcgctc cgccatctga cggcgatctg ggacaaggag
      901 gaaaaggacg acagcgtccg cttcctctcg ttcgatcccg gcgacatgga cacgccgctc
      961 cacgcgctgg ctttacccga gaccgatccg gcgacattga agcggccgga agacgcggcg
     1021 gcagaaatcg ccgaggcgat cggcgcatcg ctgcgccgcg cagagcgctt tgccggcgcc
     1081 cgcccatgat cgccgcccgg catttcgacc gccgcggcgg caagctgctt gtcgtcggcg
     1141 ccaagggggc gatgcggcgc ctgccgcgga ccgagctcgc gagcctgttc gaggccggcg
     1201 acctcatcgt cgcgaacgac gccgcaactt tgccggcgag cctgaaaggg acgcatcgcg
     1261 aaaccggcgc gccggtcgag atccggctcg cggcatggat ccggctcggc gatccgacgg
     1321 gcttcgtcgc catcgccttc ggcgccggcg atcatcgaac gcgtaccgaa gaccggctcg
     1381 cgccgccgcc gctcgcggcc ggcgaccggc tcgcgctcgg cccgctgcag gcgataatcg
     1441 agcgcgtcct cgaccatccc cgtctcctcg ttttgcgttt cctcggggag cgcgcagcca
     1501 ttcatgccgg cctcgcgcgt cacggccggc cgatccaata cgcgcatgtc ccggagccgc
     1561 tggcgctttg ggacgtctgg acgcgcatcg ccgccgcgcc ggctgcgttc gaggcgcctt
     1621 cggcgggatt cgcgctcgac tgggcgacgc tcgccgcctg gcggcggcgc cggatcggct
     1681 tcgcgacgct gactcacgcc gccggaatct cctcgactgg cgacgccctc ctcgacaagc
     1741 gcctgccctt cgacgagccg taccaagtcc cgcattcgac cgcggcggcg gtcgccgcgg
     1801 cgaaagcgcg aggagggaaa gtcgtcgcga tcggcaccag cgtagttcgt gcgctcgaag
     1861 ccgcggcgga cgaagccggc gtcattcgcg ccggggacgg cgtcgcccgc ggccgcatcg
     1921 ggcaggaaac caggctccgc atcgtcgacg ccctcctgac cggcgcgcat cagccgggcg
     1981 agagccattt cgaactgttg aaggccttcg ccgacgaggc gacgctcgaa cgcatgtccg
     2041 ccgctttcat cgcgaacgac ttccgcggcc atgaattcgg cgacagcgtg ctgatcgcgc
     2101 gcgcccggcg atcgcccgag gatcgggcga tgagcgcgtt ggtccccgcc tcgcttgccg
     2161 aatgaggcgc ttccgcccct tatggcgttt cggcggcctg tgctaagcaa gagcgctgcc
     2221 gcgcgccgac ttatcgcgac gcgaagcaat ggataggccg acgagacgca atctgatgca
     2281 gacgctgtcg atcttccaat cgctctgggc catggagcgc cgtcatacgg acgggctcga
     2341 gcgctcgctc gaagagaatg tcgcgatgat cgccgaagcc ggctttgccg gcgtcagcgc
     2401 cgattggcgc aatcgcgatt ttgttaagcg cctcgccggc ctgctcaagg cggccaatat
     2461 gcgggccgaa ggccagtgct tccccaagac cgtcgacgac ttgaagccgg tcctcgacat
     2521 cgcgacggaa ttcggcgtcc atcatctcga cattcagccg gacgtgaggc cgcgccgctt
     2581 gcaggattgc gtgtcccttc tcgaaggctg gcggcgtctc gccgaagatg tcgacttccc
     2641 ggttttcatc gagacgcatc gcgaccggat gacgaccgac ctctatttca cgctcgattt
     2701 gctcgattgc ttccccgatt tgaagctgct cgcggatctg tcgcatttcc tcgtcggccg
     2761 tgaattcgcc tggccggtct cggacgagaa ccatgcgatg atccatcgca tcctcgacaa
     2821 ttcctgggcc tttcacggcc gcgtcgccag ccgcgagcag gtgcagatcg aaatctcctt
     2881 cccgcatcac cagatgtggg tcgatctctt tctcgaatgg tgggaatacg gcttccggag
     2941 ctggcgcaag cgcgccggac caaacgacga tctcgccttc acctgcgagc ttgggccgaa
     3001 gccctatgcg atcaccggcc ccgacggcaa cgatacgaca gaccgctggc gcgaggcgtt
     3061 gctgatgaag gagaaggtcg agcaggtctg ggcgaagacg gaagcataac cgtcattgcg
     3121 agcgaagcga agcaagacgg cgctttgcga atggattgct tcgtcgcttc gcgcctcgca
     3181 atgacggata atcacgccac gccgagcttc ctttgcaggc tggtcgagga cgtcgtgtac
     3241 tggaacagca gcttcttgtc cgggtagacg tagcgatgga ccttctgcgc ggccagggcg
     3301 gcttcgtgga agcctgacag gatcagcttc agcttgccgg gataggtgtt gatgtcgccg
     3361 atggcgaaaa tgcccggcgc gctggtctcg aatttttccg tatcgaccgg cacgagattt
     3421 tcgtggaggt tgaggcccca atcggcgatc ggaccaagct tcatcgtcaa gccgaagaag
     3481 ggcagcatgt cgtcggccgg atggtcgaag acgctgccat cctcgccgcg gatcgtcgcc
     3541 gcgtgcaggc ggccggcttc gcccttgagg cccgcgacct ggccaagaat gaaatccatc
     3601 ttgccggccg cggcgagctc gcgcatcgcg ttgaccgaat gcggcgcggc gcggaacgcg
     3661 tctcttcgat gcagcagcgt gacccgctcc gccaccgggt gaaggttcaa gacccagtcg
     3721 agcgccgaat cgccgccgcc gacgatcagc acgcggcggc cgcggaactg ctcgatcttg
     3781 cgcaccgcgt aatggacgct cttgccttcg taagcgtcga tgccggcgat cggcggcttt
     3841 ttcggctgga acgagccgcc gcccgccgcg atgatgacga ccttggcttc gaaaaccttg
     3901 tcggcgtcgg tcgcgatgcg aaagagcggg cgacccgcct cgcccaagac atcgaggcct
     3961 acgaccattt cgccgagatg gaaggtcggg ccgaaaggct tgatctgggt gagaaggttg
     4021 tcgacgatgc cctggccggt caccatcgca aagcccggaa tgtcgtagat cggcttttcc
     4081 ggataaagtt cggcgcattg gccgccgacc ttgggcaaaa tgtcgatgag atgggccttg
     4141 atgtcgagca ggccaagctc gaacacggcg aagagcccga ttggcccggc gccgacaatg
     4201 acggcgtccg tcttgatgat gtcgttcatg aaaggtttcc gttggcgggc ggttggctca
     4261 accttggcgc ggcggcgtcg tcacaacgag gccgtcgagc gccggcgtca ccttgatttg
     4321 gcaggaaagc cgggaattgg gccggacctc gaaggcgaaa tcgagcatat cctcctccat
     4381 ttgctccggt tttccgacaa cgtccatcca ttcctcggcg acatagacat ggcaggtggc
     4441 gcaagcgcag gcgccgccgc attcggcttc gattccggga acgctttcgc gcagggcggt
     4501 ctccatgacg gtcgagcctt cctgcgcctc gaccgtccgg gaagcgccct cggaatcgtt
     4561 ataggtgatg cggatcatgg agctctggtt cctctatcgg cgcgagcgca tcgtgacggg
     4621 cgcgcgatat tgcaacgcgg aagccccggc cgccgccttc ataaagccag aaatcgagaa
     4681 gcggaaggcg gccgcgccgt ttccgaggcg ggccctttcg gctaggcgag cagcctggcg
     4741 atggcctccc gggcctcgct gaccgcgccg gcgagacttg acagggaggc ggcgagggcg
     4801 acctgcccgc tcccctcggc tgaagcggcc tcgacttgcg cggcggcttc ggccacccga
     4861 ttggccccga ccgccaaagc cgaacccttc agcgtatggg cgagagcggt ccggagcttg
     4921 aaatcgtcgc cgcgcgccgc cgccatctgg ccgaccaggc gcgccgcctg ctgctgaaac
     4981 atcgccagca attcgcgttc caggtcttga tcgcctaaac tttgacgggc aagatggaca
     5041 aggtcgatgg gcggcgaggg cgccgccacc gccgcggccg acggccaagc ggcgttctcc
     5101 caatctttat aaagaattgg cgcaacatcg ttcattcgcg cgcgctccgg aaatcgtgtc
     5161 gtgcggcaag aataagcgat ctccgatgaa gaaatatttg tcgcagccag ggtttcgcca
     5221 tggtccgcct tcacggttac tgaagcgtta acgacgtttg tggatcgaaa agacctaacg
     5281 tttcaatctc ggcggccggc ctgtaaactg ggcttggtta atcgactttt taagcgcgcg
     5341 cccaaaggcc acaggagggc ggcgactcgg ctcgaaccgc gcccggcgca ttgatggata
     5401 aggcgggatc aatggctcaa cagaacaagg ctcaggacgc cgtcgcggcg gccatgtcgg
     5461 cgatcgaaga agccctcaac atgtcggtgg ccgaccaggc caaagagggc aagccggccg
     5521 agccggcgga cgaggcgaag agccaagccg cgaagccgct gccgaacatc gccgcggccc
     5581 ctgccccgtc cgtcgcctcg gctgcgcccg agcggcgcgc cgcggccgag ccgcgcgcca
     5641 tgcccgcccc gatccgcccg ccaaaggccg aagcgccgaa agccgatgcg ccgagcctca
     5701 cgccgccgcc cttgccgagc gccggcgagc ctccagccgc cccgccgccg cccttgccga
     5761 cgaccccgcc ggccaatgac gaccggcctt ccgtcggcca gcttctgctc gcttttcaaa
     5821 gccggccgcc gagccgcgcg ccggtgatcg cggcgctggc ggcctcggtc ctttggcttt
     5881 tgttctgcgg cgcgtttctg gctcagcacg gcgatctttt cggcgggcgc tcgccggtaa
     5941 gccgcgaatt ctggctccag ccgcaaatcg cgctcgccgc gctggtcgtc gtcggccctg
     6001 tcctcttcat cttcgcaatc gcggcgctgg ttcgccgctc gcaggagctg cgcggcgccg
     6061 cgcgggcgat cagccaggtc gccatgcgcc ttgccgagcc ggagaccgtg gccagcgagc
     6121 aggtggcgac gctgtcgcag gcgatccggc gcgaagtcgc ctcgatgggc gacggcatcg
     6181 agcgcgctct tgcgcgggcc tcggagctcg aaacgctggt gcgcggcgaa gtctccaatc
     6241 tcgaacgctc ttattcggac aatgagcggc gcgtccgcgc tttgatcgcc gaattggccg
     6301 atcagcgcga ggcgatcgtc gccaatggcg gcaaggtccg cgccgcgatc gccggcgccc
     6361 atcagggcgt cgccgccgat ctcgacgcgg tcgccgaccg gctcaacgag cgcatcctca
     6421 ccgccggcca aggactggtc gcttcgctcg gcgcgaccgc cgaaggcgtc ggcgtcgcgc
     6481 tggagcgggc cggcgccgac acgatcgaaa agatcggcag ccacagcgcc catctgaccc
     6541 aaacgctcgc ggccgtcggg gccgatgtcg ccaatcgcct cgccgaagcg accgaccgca
     6601 acgccgcttc gctgatcgag cgcgtcgccg acatcgatca gcgcgtcaag agcacgggcg
     6661 aggccctgac ggccgatctg acggtgcggg gcgaggactt ggtcggtcgg ctcgacgccg
     6721 ccggcgcccg cgtctccgac gcgatgatcg gccatggcga gagcctggtg gcgcgggtcg
     6781 ctcagacgag cgaggcgatg ctggccgatt tcaccgcccg cgccgacgac gtcgccgcgc
     6841 gtctcgacaa taccggcggc cgtatcgccg agacgatcct tgaccgcggc gagggcctgg
     6901 tgtcgcgcct cgccgagacg accgaccgca tgcatgaagt cgtcgcggtg cgcggcgaac
     6961 aattccacgc tcggctcgcc ggcgcgcacg atcaactcgt cgccgccttg agcgagcgcg
     7021 ccgacgacgc ccagcgcctg ttcgagtcga ccgccgctaa ttgggccgcc gaattcgacg
     7081 cgcggcacgc cgcgcttggc gcccagctcg aacagcacgc cagcgacgtc aacgcccgtt
     7141 tcgaagagac ctcgcagcgc acgatcgccg cgatccgcga caacagcgcc gagatgcacg
     7201 agcgcttggc gacgaccgcc agcgattcga tcgcgcgcat ggacgagcag acgagccgtc
     7261 tcgccgaccg tttcgcggcc agcgccggcg aggcggtggc ggcgatcgcc atccatagcg
     7321 agagcgtcaa cgagacgctg aactcgcagc tcgccaattt cgagcagact ttctccggcc
     7381 gcggcgccgc cgccatctcg gcgcttgccg atcagaccgg ccggctcggc gagcagctcg
     7441 aaacgctcga ccgcctggtc ggcgccgccg gcgaaggctt cgtcgagcgg atcggcgcgc
     7501 atgccgaccg cgtgacggcg catatcgatc agcagatcgg cgcgctcgac ggcgcaatgg
     7561 ccggccgcag cgccgagatc gaccgccgcc tcgccgagca cagccagaag ttcatggaag
     7621 tttccggcgc caggctcgcc gaattcgaaa ccgcctcggt cgcctatcac gccgatctcg
     7681 accgcgcttt ggcgtcgcat tccgagcgcg tcgacaacac gatccgagaa ggcattgccg
     7741 gtttcgaatc gatatccggc gcgcgccata tcgatcttgc gacggcgctc ggcgcccatt
     7801 cggcgcgcgt cgacaatgcg atgcgcgagg gcctcgccgg actcgaaacg gtttccggcg
     7861 cccgccaggc cgatctcaac gccgctttgg cgtcccatac gtcgcgggtc gacgcgacgc
     7921 tgcgcgacgg ccttgccggg ttcgaatcgg ttgcgctcgc ccatcatgaa aatctcaccg
     7981 gcgcgttggg cgcgcattcg agccggatcg acagcacgat ccgcgacggt ctggccgggt
     8041 tcgaagccgc gacggcggcg cgccagaccg aactcagcga agtcctcggc tcgcatgcgg
     8101 agaggatcga cggcgcgatc cgcggcggtc ttgccggctt cgagaccctg gccgcgtctc
     8161 accacggcga tctcagcgaa gcgctgaacg cccataccgg gcgggtcgac agcgccatgc
     8221 gcgaaggcat agccggattc gaaaccgcct cggcggcgca tcatgccgaa ctcaacagcg
     8281 cgctggcgac ccattcggag cgggtcgagc acgcgctccg cgaaggcctt gtcggcttcg
     8341 acgcggcttt gtccggccgc gtcggcgaca tggtcgatcg cgtcgagagc cagaccgacg
     8401 ccttggtcgg ccggatcggc gacaaattgt cggcgatcga ggagacgttc accgtgcacg
     8461 ggccggcgct cgatgcgcgg ctcggcgcgc gcagcgccga ggcgatcgcc ctcttcgaga
     8521 acaatctcaa gacgctcgac gatcgcaccg cggtgcgcag cgagcatatt tccaagacga
     8581 tggagaccct gctcgaccgc gtcgacaacg gattggagac gcgggcgcgg gcgctcaacg
     8641 acacgctcgc ccagcgtacg ctcgaaatgg cgaggacgat gagccttggc ggccgcgagg
     8701 tcgtaagcgc gctcgacgcc aagtcgaagg agatcggcga agccttgcaa agccgcgcgg
     8761 cgaccctgac cgaagcgctg tcgggccagg cgcgcgaaat caacgcgacg ctcggcggca
     8821 atttgatcgc gctcagcggc gcgctcggcg aaagcgtcga tcgcatgcag gagcgcgtcg
     8881 tcgcgccgct ggaaggcctt tcgacgaccc ttggcgccct ggtctcaggt catgccgaca
     8941 gcatcgccgc cacgctcggc gagcatcgca aagtcatcga cgagacgatc ggcgcgcatg
     9001 gcgcggcgat ccgccggtcc ttggagagcg gctccgccga gatcgagacc ggcatggcca
     9061 aggcgatcgc cgtcctcgag agcgctttcg cggcgcatgg cggcggcctc agcaagacga
     9121 tcggcgagca gatcgacgat ctgcgctcga tcgtcgagga caagggcggc gatttcatcg
     9181 cccggctcgg cgcgcgcggc ggcgacatgt cggccgagct cgccaacgcc gccgaccgcg
     9241 ccgcgcaggc cttcgatcag cgcaccaccg cgctgatcaa tctcctgacg aggcgcagcg
     9301 aggacctgct cggcgcgctc aacgcgggct cggccgcgtc gctgcaatcg ctcggcgcct
     9361 tgaccgcgca gatcggccag gacgcgcaaa acgccgccgc tctgctgcgg accgcggccg
     9421 acgagaccgc ggcgagcgcc tcgcaggcga tcagcgggct cagcgaaaag ctgcgccagg
     9481 aggtcgagca atcggccagc cagttgcggg agaccctgga gcgcagcggc ggcgcgtcgg
     9541 taaacgcgct gagcggcgcc ggcgaacgcc tgcgcaacga attgctgcag gttctcgatc
     9601 ggctcggcgc gaccagttcg acgctggagc gagtggtcga atcggcgagc tccgatctcg
     9661 aagcggtccg cggcggcctc gccgaccggg tcggcgaatt cgagcgggcg ctgggcgcga
     9721 tctcctcgca gattgccgcg ctcggccgcg cctcggcgac gacgcagaag gaggccgccg
     9781 gccttgccga tcgcctcgcc gatcacgtca attcgttggc cggcgcggcg catgatctcg
     9841 cctcgatgca gaacacgttc gacgacgcca tcgcgcggcg gcgcgacagc ctcgccaatc
     9901 tgatcgagga ggtcgcctcg aagagcgacg cgttcgagaa cttgatgcag accttcacga
     9961 cgacggtcga agattcgctc aatcgcgcgc tgcagcgggc gaaagacgtg cagacctcga
    10021 tcgcccagac gaccagcggc gcggcgcggc agattaccga tcaattcgag acgatccgcg
    10081 atcacgccgg caaggagcgc gagcgcaccg ccgccgcgct ggaagaagcc tacgagcagg
    10141 ccaccgcgca gctctcgacg attatgaatc tcgccaatga ccgcttcaag cagaccgccg
    10201 caagcttgcg cgcgaccacc gtcgagatca cccgcgaact ggaggcgacg cgccaggaat
    10261 tgcagcgcgg cgtgctcgaa ttgccgcgcg agaccagcga gcaggccgcc gcgatgcgcc
    10321 gcgtcgtcgg cgatcagatc aaagccctga acgagctcgc cgagatcgtc gcgcgcgccg
    10381 gcgggacgtt cgacgtcgcc gagcccgccg ccccgccgat cgcggcgccg ccccgcgccc
    10441 tcgaagcgcc gcgggccgag ccggcccgtc tgcaggcgga gcctgcgccg caagagccgc
    10501 cgcgaccgcg tccggccgct cgctcgcccg cggccaatcc ggcgccgagc gccgcggcgc
    10561 gcggacaagt cggctggctc agcgacctgc tgtcgcgcgc gtcgcgcgag gagagcgagc
    10621 ccgccgtccc ggccggcgcc gaaagccggc cgagcgtcga gcagttcgat ggaatttcgc
    10681 tcgacatcgc ttcgctgatc gatccagcgg cggccgccga aatgtgggac cgctggcgcc
    10741 agggcgacac cagcgccttc tcgcgccggc tctatacggc gcaaggccag cagacgttcg
    10801 acgagatccg ccgccgctac aaggccgacc cgcaattccg cgagacggtg acgaaatacg
    10861 tccaggaatt cgagcgcctg ctcgccaaga tcggccgcga cgaccgcgac ggctcgcatt
    10921 cgcgcgaata cctcctctcc gacaatggcc gcgtctatac gatgctggcg cacgcctcgg
    10981 ggcgcttggg ctgagcggcg ggcggcgctc tcggcggaaa cgccggagag cgtcgccgcc
    11041 ggcggcggat ttctcggccg ctgacgctgc gcggccccct ccccgcgcct gaatttcgcc
    11101 gcggcgccgt caagcgccct cggcgcctct tgaaacagcc gcttcgtttt ctagctcaat
    11161 agcgagccgc gcgggacgcg cagcgttggc caaaaagggc ggcgcgggac cagtgtccgg
    11221 cgcgggacgg agacgtatgc atggaaagga ggtaggttga gatggccctt agagatctga
    11281 ttccgggcgg cggcgggcgc gacgtcgcca cgcgccgcag cgatccgttc ttcgccttgc
    11341 agcgggatat gaatcgcctt ttcgacgatt tcttccgcgg cttcgacctg acgccgttca
    11401 gcggcgatca cggcgcggga tggccgaaca tcgaggtgag cgaaaccgaa aaggacgtga
    11461 aggtcatggc ggaattgccc ggcctcgacg agaaggacgt tcaggtcgat ttcgccaatg
    11521 gcgtgctcgc cataaagggc gagaagaaga ccgagaccga cgacaaggag cggctgttca
    11581 gcgaacgtta ttacggccga ttcgagcgcc gcatcccggt cgacgacgtc gatgaagaca
    11641 aggtttccgc cacgttcaaa aacggcgtcc tgaccgtgac gatgcccaaa gcggcgcaat
    11701 ccaaggtcaa gcggatcgcc atcaacggca aatgaccgga cgggagcgac cgctcccgcc
    11761 cgcctcggcc tgtgcgcaac cgcgatgaca atcgccgcgg cgcggcgccg ttgggacggc
    11821 gccggctgcg gcggattgtc tgcggctcaa aacgactaaa cagttcgccg gttcgaactt
    11881 cgtaccgaga agggcagcat cgccgtcggt caaaggccct cgcatatttg tctcgaaccc
    11941 taactaaagc cccactgcag gtgagcggcc gatctctatt ttaagcaaac ggcggaccga
    12001 gccgggcccg aaggccgctt gcgcggacgc gcctcgcgtt gcagagtggc gggctatggt
    12061 tctcctgctc tacggctatc cgaagaagag cggctggtgg ccgccgccgg ccgcttcacg
    12121 ctgactgccg tttcccttga gcttcaggag ccatccacgc gaatccgtaa cgcggagccg
    12181 cgcgaacgcg cgctcacgat ccattggtcc gacggcgccg ccgccgactt cccgtatctt
    12241 ttcctgcgcg acaattgtcc ttcgggcttt cacccgcaaa cgcaggagcg gttattcgat
    12301 ctgctcagca tcccggacga gttgaagccg cgccatgtcg cgaccgacgg gcggacgctg
    12361 acgatcgact ggcccggcga gccgtcccac cggactgtcc tcgacgcaga ttggctcgcc
    12421 gatcatcggc ccggcgcgag gccgccggac cccgccaata tcgtttcggt cagttggggc
    12481 ggagatttcc tgccgcgcct gccccgccga gaagccgcag cgctcgcgcg tgatccggcg
    12541 gcgatgaaag actggctcat cgccctcaag cgcgacgggc tctcggttgt gaccggcctc
    12601 gccgacgatg aggaggccgg cgtcgaattc ggcaagacga tcggatttct gcgccggacg
    12661 aatttcggcg agacgttcag agtcgagaca aagcccgatc cgaacaatct cgcctatacg
    12721 tcgcacgccc tcgctttgca taccgacctt cccaatcaag agatgccgcc gggtttccag
    12781 ttcttgcatt gcgtccggaa cgacgcggcg ggcggcggct caattttctt ggacagcttc
    12841 cgcatcgccg agcgtttgcg cgaaagcgat ccgaagggat ttgcgctgct ttcgaccgta
    12901 cccattccct atcgtttcca cgaccgcgaa tacgacatcc gcatcgaccg gccgctgatc
    12961 gggcttgacg aaagaggccg cctcttcgac gtccgattca gcgcccat
//
LOCUS       DATGJW010000467         7254 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1336291, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000467 DATGJW010000000
VERSION     DATGJW010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7254)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7254)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7254
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1336291"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..490)
                     /locus_tag="VKU03_14080"
     CDS             complement(<1..490)
                     /locus_tag="VKU03_14080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020086533.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015658 - branched-chain amino acid
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HLJ72444.1"
                     /translation="MAGKFLGAAALALAVCFAAGGAEAQVKFGVAGPITGANAAFGAQ
                     LTNGTELAVEDINAAGGILGQKIVVETGDDVSDPKQGVSVANKFVGDGIKFVIGHFNS
                     GVTIPASDVYADSGVLFITPSATNPKVTERGLWDAFRTCGRDDQQGKLWADYALQRLK
                     GKK"
     gene            complement(703..1632)
                     /locus_tag="VKU03_14085"
     CDS             complement(703..1632)
                     /locus_tag="VKU03_14085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018389015.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HLJ72445.1"
                     /translation="MTTILLTHPASLDHHAGYGHPERPERIQAIERTLEEERFALLAR
                     ETAPLVSREALLRVHTEPYLAAIESAAPQEGLRRLDADTAMSPGTLEAARRAAGAATR
                     AVDAVMSGRCANAFVAMRPPGHHAETETPMGFCFFNNAAIAARHARAIHGAERVAVVD
                     FDVHHGNGTQHIFWGDKDAMYCSTHEMPLFPGTGAINERGEHDQIVNAPLRAGDGGAA
                     FREAFDIALLPRLEGFRPDLVIVSAGFDAHRLDPLGHLNLVEADFAWATRRLMDIAGR
                     ACKGRIVSLLEGGYDLDGLARSVAAHVGALMEG"
     gene            complement(1767..2369)
                     /locus_tag="VKU03_14090"
     CDS             complement(1767..2369)
                     /locus_tag="VKU03_14090"
                     /inference="COORDINATES: protein motif:HMM:NF018561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1254 domain-containing protein"
                     /protein_id="HLJ72446.1"
                     /translation="MKRAAATMRRTVIDIATIVLGAALTAGLVHIVAILIIPFYASND
                     AFARLSNLGPIDATIMLPEAGSPAARLFPNLDPAVAAAFCRYDLTDGPVRVKAPVARY
                     EFSSLSFHTRRGQIYYALTDRAATQNALEALVVTDKQLRAMSAHDSEDETPLELRIVS
                     ATNQGFVLMRAFSQEPSLLAEAQERVRALKCQPEPQPQPR"
     gene            complement(2366..2944)
                     /locus_tag="VKU03_14095"
     CDS             complement(2366..2944)
                     /locus_tag="VKU03_14095"
                     /inference="COORDINATES: protein motif:HMM:NF018452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1214 domain-containing protein"
                     /protein_id="HLJ72447.1"
                     /translation="MDVLAKTLIAGALGLAAGLWSAESALRLSRGAETTRIGAWSTAA
                     KAGTPEADPYTRDTIERSGEIPLAPGEGLQLVARVDDSGTALNPHCVYRIGRKVPAAR
                     FWTIGVVDRRGFPIPNPAQRLVFRSSEILRDSDGGFAIAVSASAHAGNWLPIGVSEPF
                     YLVLRLYDTPFSATATAIDKDALPRIVRESCR"
     gene            complement(3001..4020)
                     /gene="ilvC"
                     /locus_tag="VKU03_14100"
     CDS             complement(3001..4020)
                     /gene="ilvC"
                     /locus_tag="VKU03_14100"
                     /EC_number="1.1.1.86"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018319725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ketol-acid reductoisomerase"
                     /protein_id="HLJ72448.1"
                     /translation="MRVYYDRDADLNLIKGKKIAVVGYGSQGHAHVLNLRDSGAKEVA
                     TALREGSSSAKKAAAEGIKVLSVAEAAKWADVVMMLTPDELQADIYAKELAPNMKKGS
                     ALLFAHGLNVHFNLIEPRADLDVLMVAPKGPGHTVRSEYLRGGGVPCLLAIHQDASGN
                     AHDLGLSYAAAIGGGRAGVIETTFREECETDLFGEQVVLCGGLVELIRNGFDTLVEAG
                     YAPEMAYFECLHEVKLIVDLIYEGGIANMNYSISNTAEYGEYVSGPRIITPETRAEMK
                     RVLADIQSGKFTRDWMLENKVNQTSFKAMRAKHNSHQIEAVGEKLRAMMPWIGKNKLV
                     DKAKN"
     gene            4342..4719
                     /locus_tag="VKU03_14105"
     CDS             4342..4719
                     /locus_tag="VKU03_14105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72449.1"
                     /translation="MNSGIDIPSTAENEVTLRAAAAVEAVVATGAALLVAEPWRASRI
                     VRGAESMPLDALRAEVARRRALPPPADFNRALALAQLVRALDDEAFAAAWALTVGPPG
                     SADQISSVSSCAALSAASASHPA"
     gene            complement(4656..6485)
                     /locus_tag="VKU03_14110"
     CDS             complement(4656..6485)
                     /locus_tag="VKU03_14110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006019804.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016866 - intramolecular transferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HLJ72450.1"
                     /translation="MADALNLFPPKSEDHWRRRVATALKGAGFEKLISQSEDGFAIAP
                     LYERERGPRAGRPGGPWRVLARADHPNAETANAQAVDDLARGADGLEIVFAGAGEAYG
                     YGLSQSDAASFDILFRNVRFDNARIELDPGAAGEEAARNLADHVRLNGEPHRADIGFG
                     LDPIGRLALSGRGRAWSEEAAALARFARDLAAQSFVGPFVAADGRIVHAAGGSPAQEL
                     AFALSCAVTYLRALEAVGVALDEARCAIGFRLAADADEFISLAKFRALRLLWTRIEAA
                     CGLTPKSVRLHASSAWRMMTARDPYVNVLRGAMAAFAAGLGGADSISVLPFTQAIGLP
                     DAFARRLARNAQLILLDESHLGFVIDPAGGAGAFEALTEKLCEKAWSLFQSLEAEGGI
                     VAALERGAFQADVAAAADKRARHVARRKAAMTGLSDFPDLAEIKPETLAAARPRFAYP
                     GEARCAPLPPRRLAEPFEALRDASDARLKTKGARPRVFLANLGPVAAFNARANFAKSL
                     FEAGGVEALGNDGFADGAQAAAAFKASGAALACLCSSDEIYAASAEGAARALREAGAR
                     VWMAGRPGAAEARLREAGVTNFIYAGCEALAALSAAHELTDEI"
     gene            complement(6541..6948)
                     /locus_tag="VKU03_14115"
     CDS             complement(6541..6948)
                     /locus_tag="VKU03_14115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2267 domain-containing protein"
                     /protein_id="HLJ72451.1"
                     /translation="MENLIARVADAAGVTPDVARQATGLVADFIRREAPNEAVDKLLE
                     KAPALKSIIASLPDSGGEGMSGGIKGLMGIAAGAMGGGGLMALGGQLMSLGLDMGQIQ
                     TIGKEIFAYAREVAGDDVVGEIAGAIPGLSQFI"
     gene            complement(7035..>7254)
                     /locus_tag="VKU03_14120"
     CDS             complement(7035..>7254)
                     /locus_tag="VKU03_14120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018262683.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sodium-independent anion transporter"
                     /protein_id="HLJ72452.1"
                     /translation="TTIAGFARKARRRGALVFIAGASGAVRRALLTHGVRPPDVRYRA
                     RLPDAFAAARRETAADPPEPAAARPATA"
BASE COUNT         1195 a         2371 c         2484 g         1204 t
ORIGIN      
        1 tcttcttgcc cttcaggcgc tgaagcgcgt agtcggccca gagcttgccc tgctgatcat
       61 cgcggccgca ggtgcggaac gcgtcccaca ggccgcgctc agtgactttc ggattggtcg
      121 ccgacggcgt gatgaacagc acgccgctat cggcataaac gtccgaagcc ggaatcgtca
      181 cgcccgaatt gaaatggccg atgacgaatt tgattccgtc gccgacgaac ttgttggcga
      241 cggagacgcc ctgtttgggg tcggagacgt cgtcgccggt ctcgacgacg attttctgcc
      301 cgagaatgcc gccggcggcg ttgatgtcct cgaccgccag ctcggttcca ttggtcaatt
      361 gcgcgccgaa agccgcgttg gcgccggtga tcggcccggc gacgccgaat ttgacctgcg
      421 cctcggcgcc gcccgccgcg aagcaaaccg ccagagcgag ggcggcggcc cctaggaatt
      481 tacccgccat tctgacctcc caaaggttga atggcggtta agctaaggct gattgtttaa
      541 cctgtaaaga gtattttgaa ggttatgggc gcctgtcatt cccgcaagcg ggaatccagc
      601 atggccgcat cccgcaagcg cggcggcgcc ctggattccc gcttttcgcg agagtgacgc
      661 gggcttactg ttgaatttgt ccgtcgccgc gatgcggggc gctcacccct ccatcagcgc
      721 gccgacatgc gcggccacgg agcgggccaa gccgtcgagg tcgtagccgc cctccagcag
      781 cgagacgatg cggcctttgc aggcgcggcc ggcgatgtcc atcaagcgcc gcgtcgccca
      841 ggcgaaatcc gcttcgacca gattgagatg gccgagcggg tcgaggcgat gggcgtcgaa
      901 tcccgccgag acgatgacca ggtccggccg gaaaccttcg aggcgaggta gaagcgcgat
      961 atcgaaggcc tcgcggaacg ctgcgccgcc gtcgccggcg cgcaatggcg cgttgacgat
     1021 ctgatcatgc tcgccgcgct cgttgatcgc gccagtgccg ggaaagagcg gcatctcatg
     1081 ggtcgagcaa tacatcgcgt ccttgtcgcc ccagaaaatg tgctgcgtgc cgttgccgtg
     1141 atggacgtcg aaatcaacaa cggcgacgcg ctcggcgcca tggatggcgc gcgcgtggcg
     1201 cgccgcgatc gcagcgttgt tgaagaagca gaagcccatc ggcgtctcgg tctcggcgtg
     1261 atggcccggc gggcgcatcg cgacgaaagc gttggcgcag cgcccgctca tcaccgcgtc
     1321 gacggcgcgc gtcgcagcgc ccgcggcgcg acgcgccgcc tcgagcgttc ctggcgacat
     1381 cgccgtgtcg gcgtcgaggc ggcgcaagcc ctcttgcggc gcggcgctct cgatcgcggc
     1441 gagataaggc tcggtatgga cgcgcagcag cgcctcgcgg ctgaccagcg gcgccgtctc
     1501 gcgcgcgagc agcgcaaagc gctcctcctc aagcgtccgc tcgatcgcct ggatgcgctc
     1561 cgggcgttcg ggatggccat agcccgcgtg atggtcgaga gacgctggat gcgtgagcag
     1621 gatcgtcgtc acggcgtccc tcctttgcgc ggcgtcggcc gcgctgcgct tcaagatcgc
     1681 gccaggcagc ggggctgtcc atcggcgcaa aacgaatttg tgaaggatag gacgagaatg
     1741 tggagcggtc gcgaccgcgc cggcgtctac cggggctggg gctggggctc gggctggcat
     1801 ttcagcgcgc ggacccgctc ctgcgcctcg gcgagcaggc tcggctcctg gctgaaggcg
     1861 cgcatcaaaa cgaagccctg attggtcgcg gacacgatcc gcaattcgag cggcgtctca
     1921 tcctcgctgt catgggcgga cattgcgcgc aattgcttgt cggtgacgac cagcgcttcg
     1981 agcgcgttct gcgtcgcagc gcgatccgtg agagcataat agatttggcc gcgccgcgta
     2041 tggaaggaga gcgacgagaa ttcatagcgc gcgaccggcg ctttgacgcg aaccgggccg
     2101 tctgtgaggt catatcggca gaacgccgcc gcgaccgccg gatcgagatt ggggaagagg
     2161 cgggctgcgg gcgaacctgc ttcggggagc atgatcgttg catcgatcgg gccgagattt
     2221 gataggcgcg caaatgcgtc gttcgaagcg tagaacggga tgatcaagat ggcgacgatg
     2281 tggaccagcc cggccgtcag cgcggcgcct aagacgatcg tcgcgatgtc aatgacggtc
     2341 cggcgcatcg tcgcggccgc gcgcttcatc ggcagctctc cctgacgatg cggggcaggg
     2401 cgtccttgtc gatcgccgtg gcggtcgcgc tgaacggcgt gtcgtagagg cggagcacga
     2461 gatagaacgg ctcgctgacg ccgatcggca gccaattgcc cgcatgggcg ctcgccgaaa
     2521 ccgcgatggc gaagccgccg tcgctatcgc gtaagatttc gctcgaccgg aagaccagcc
     2581 gctgcgccgg attgggaatg ggaaaaccgc gacggtcgac gacgccgatc gtccaaaaac
     2641 gcgccgccgg aactttacgg ccgatacgat agacgcaatg cgggttgagc gcagtcccgg
     2701 aatcgtcgac ccgcgccacg agctgcaagc cctcgccggg cgcgagcgga atttcgccgc
     2761 tgcgctcgat cgtgtcgcgc gtgtaaggat cggcttccgg cgtgccggcc ttggccgcgg
     2821 tcgaccaggc gccgatccgc gtcgtctcgg cgccgcgcga caggcgcaag gccgattccg
     2881 ccgaccacag gccggcggca aggccgagag cgccggcgat gagcgttttg gcgagaacgt
     2941 ccaaagagta cgtccggagc ttcccttccc cgcttcgcgg gggagggaag acgccagccc
     3001 tcagttcttc gctttgtcga ccagcttgtt cttgccgatc cacggcatca tggcgcgcag
     3061 cttctcgccg actgcctcga tctgatggga attgtgcttg gcgcgcatcg ccttgaacga
     3121 ggtctggttg accttgttct cgagcatcca atcgcgggta aatttcccgg attggatgtc
     3181 ggcgaggacg cgcttcattt ccgccctggt ctccggcgtg atgatgcgcg ggccgctgac
     3241 atattcgcca tattcggcgg tgttggagat ggaatagttc atattggcga tgccgccttc
     3301 ataaatgagg tcgacgatca gcttcacttc gtgcaggcac tcgaaatagg ccatttccgg
     3361 cgcatagccg gcctcgacca acgtgtcgaa gccgttgcgg atcagttcga cgaggccgcc
     3421 gcaaagcacg acctgctcgc cgaagagatc ggtctcgcat tcctcgcgaa acgtcgtctc
     3481 gatcacgccg gctcgtccgc cgccgatcgc cgcggcatag gagaggccga gatcatgggc
     3541 gttgccgctc gcgtcctgat ggatggcgag caggcagggc acgccgccgc cgcgcagata
     3601 ctccgagcgg accgtatggc cggggccttt cggcgcgacc atcagcacgt cgagatcggc
     3661 gcgcggctcg atcagattga aatggacatt gagcccgtgc gcgaagagca gcgcagagcc
     3721 cttcttcata ttgggcgcga gctcctttgc gtagatgtct gcctgcaatt cgtcgggcgt
     3781 cagcatcatc acgacatccg cccatttcgc ggcttcggcg acgctgagca ccttgatgcc
     3841 ttccgccgcc gcctttttcg ccgaagaaga gccctcgcga agcgcagtcg ccacttcctt
     3901 tgcaccgctg tcgcgaaggt tgaggacatg ggcgtggccc tggctgccat agccgacgac
     3961 cgcgatcttc ttgcccttga tcaggttgag gtcggcgtca cgatcataat aaacgcgcat
     4021 gttcgatcct tcgctggcgt ttgaagaacg ctgggctttg gcggaaaatc tcgtgcggcc
     4081 cgcattgggc caagccgaag cctcataggg aaaaggctcc gcctgatcaa gcgatagcga
     4141 tcggagcgaa acggtccgcg cagtgtgatt cccgcggaac gcgggaatcc ggcgcggcct
     4201 atcctcggac gcggcggctc tctcgattcg cgcttgcgcg aacgacagca ggcgcgccta
     4261 acgcccggag aacgtttgcg tattcagaaa aatagaaaat ttgtttggaa actttctgat
     4321 tttcggaata ttcttaggcc gatgaactcc ggcatagata ttccctccac ggccgaaaac
     4381 gaagtcacac tgcgcgccgc tgcggccgtc gaggcggttg ttgcgaccgg cgccgcgctg
     4441 cttgtcgccg agccttggcg ggcgagccgc atcgtccgcg gcgccgaaag catgccgctc
     4501 gacgcgctcc gagccgaggt ggcgcggcgg cgggctctgc cgccgcccgc cgacttcaac
     4561 cgcgccctcg ccttggccca gctcgtccgc gccttggacg atgaggcctt cgcggcggcg
     4621 tgggcgttga cggttgggcc gcccggatcg gccgatcaga tctcatccgt cagctcatgc
     4681 gccgcgctga gcgcggcgag cgcctcgcat ccggcataga tgaaattcgt cacgccggcc
     4741 tcccgcaggc gcgcctcggc tgcgccgggc cgtccggcca tccagacgcg cgcgccggct
     4801 tcgcgcaggg cgcgcgcggc tccttcggcg ctggccgcgt aaatctcgtc cgaagagcac
     4861 aggcaggcga gggcggcgcc gctcgctttg aatgcggctg cggcctgcgc gccatcggcg
     4921 aagccgtcat tgccgagcgc ttcgacgccg ccggcttcga acaggctctt ggcgaagttg
     4981 gcgcgggcat tgaacgcggc gaccggaccg agattggcga ggaacacgcg gggcctagcg
     5041 cctttcgttt tgagcctagc gtcggaggcg tcgcgcaagg cttcgaaagg ctcggcgagg
     5101 cggcggggag gaagcggcgc gcaacgcgcc tcgcccggat aagcaaagcg cggcctggcg
     5161 gcggcgagcg tctccggctt gatttcggcg agatcgggaa aatcgctgag gccggtcatc
     5221 gccgccttgc gccgcgcgac gtggcgggcg cgcttgtcgg cggccgcggc gacgtcggct
     5281 tgaaacgcgc cgcgctccag cgccgcgaca atgccgccct ccgcttcgag actttggaac
     5341 aggctccacg ccttctcgca aagcttttca gtcagcgctt cgaacgcgcc ggcgccgccg
     5401 gccggatcga taacgaagcc gagatgggat tcgtcaagca gaatgagctg cgcattgcgg
     5461 gcaaggcgtc gggcgaaagc gtcgggcagg ccgatcgcct gcgtgaacgg caggacgctg
     5521 atgctgtcgg cgccgccgag gccggctgca aaagcggcca tcgcgccgcg caggacgttc
     5581 acgtacggat cacgcgccgt catcatccgc cacgcggaag aggcgtggag gcgcacgctt
     5641 tttggcgtca agccgcatgc ggcctcgatc cgcgtccaga gtagccgcaa ggcgcggaat
     5701 ttcgccaagc tgatgaattc gtcggcgtcg gcggcgaggc gaaagccgat cgcgcagcgc
     5761 gcctcatcga gcgcgacgcc gacggcctcc aacgcgcgca ggtacgtcac ggcgcaagaa
     5821 agcgcgaagg cgagctcctg cgccggcgag ccgccggcgg catggacaat gcgcccgtcg
     5881 gccgcgacaa aggggccgac gaagctctgc gcggcgaggt cgcgcgcgaa acgggccagc
     5941 gccgccgcct cttcgctcca ggcgcggccg cgcccgctca gcgccaaacg gccgatcgga
     6001 tcgaggccga agccgatatc ggcgcggtgc ggctcgccgt tgagccggac atgatcggcg
     6061 aggttgcgcg ccgcctcctc gccggccgcg ccaggatcga gctcgatccg cgcattgtcg
     6121 aaccgtacgt ttctgaacag gatgtcgaag gaggccgcgt cgctctggga gaggccatag
     6181 ccataggctt cgccggcgcc ggcgaagacg atttcaagcc catcggcgcc ccgcgcgaga
     6241 tcgtccaccg cctgggcgtt ggcggtttca gcgttaggat ggtccgcgcg ggccaagacg
     6301 cgccacggtc cgccgggccg gcccgcgcga gggccgcgct cgcgttcata gagcggagcg
     6361 atggcgaatc cgtcttcgct ttgggagata agcttctcga acccagcgcc cttcagcgcc
     6421 gtggcgacgc gccggcgcca gtggtcttcg cttttcggcg ggaaaagatt gagcgcgtcg
     6481 gccatggggc gctgggcggg gtcgctgcag ccgcggtcgc tgaccgcggc tgcgcccaac
     6541 tcaaatgaat tgcgacaggc ccggaatggc gccggcgatt tcgccgacga cgtcgtcccc
     6601 ggcgacctcg cgggcatagg cgaagatttc ttttccgatc gtctggatct ggcccatatc
     6661 gaggccgagg ctcatcaatt ggccgcccag cgccatcagg ccgccgccgc ccatggcgcc
     6721 ggcggcgatg cccatcaggc ccttgatgcc gccgctcatg ccttcgccgc cggaatccgg
     6781 caaggaggcg ataatcgatt tcagcgccgg cgctttctcc agcagtttgt cgaccgcctc
     6841 attcggcgcc tcgcgccgga tgaaatcggc gacgaggccg gtcgcctgac gcgcgacatc
     6901 cggggtgacg ccggcggcgt cggcgacgcg cgcgatgaga ttttccatgc ccttctccct
     6961 gcccggccga gcggccgttg atcttagcct gtgtcgccgc tcggcgcggc aaaatcaagc
     7021 gacgcgttgg cgggctacgc ggtcgccggg cgggccgccg ccggctcggg cgggtcggcg
     7081 gccgtctccc ggcgggccgc ggcgaacgcg tcgggcaagc gggcccgata gcgaacatcg
     7141 ggcgggcgca cgccgtgggt gagcaaagcg cgcctgacgg cgccgctggc gcccgcaatg
     7201 aacaccaaag cgccgcgccg ccgcgccttc cgggcgaagc ccgcaattgt cgtc
//
LOCUS       DATGJW010000468         5692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1229207, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000468 DATGJW010000000
VERSION     DATGJW010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5692
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1229207"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(319..609)
                     /gene="rpmB"
                     /locus_tag="VKU03_14125"
     CDS             complement(319..609)
                     /gene="rpmB"
                     /locus_tag="VKU03_14125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L28"
                     /protein_id="HLJ72453.1"
                     /translation="MSRRCELTGKAVQTGNKVSHSNRKTRTRFLPNLCNVTLISDSLA
                     RSVRLRVAASSLRSVEHRGGLDNFLLKAADGELSAGALALKSEIKKKLAVAS"
     gene            827..1291
                     /locus_tag="VKU03_14130"
     CDS             827..1291
                     /locus_tag="VKU03_14130"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ72454.1"
                     /translation="MLAIRTPNVGELPALGDLCMRSKAVWGYDEKFMAACRTELSFDA
                     FDLLVTRIAVVEQDNKLLGVAQITLVDTEAHLMKLFVDPTALRHGAGKVLFAWAVAAA
                     KDMGARRLLIDSDPAAASFYRRMGARDAGLAPSGSIAGRMLPKLLFALGSAT"
     gene            1300..2193
                     /locus_tag="VKU03_14135"
     CDS             1300..2193
                     /locus_tag="VKU03_14135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332353.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phytoene/squalene synthase family protein"
                     /protein_id="HLJ72455.1"
                     /translation="MTDEDRLDQAYAHCDALTRAHDRDRWLAALFAPAEARRHLHALY
                     AFSYEVGRLRDFVREPMAGEIRLQWWREAIEGERRTEAQASPVAAALIDTIARFRLPI
                     QAFDSMLAARVFDLYDDPMPDVAAFEAYCGETSSLLFRLASLILAGRDNGGADAAGHA
                     GVACATAGLLRALPIASMRGQVYLPMDILSENGVSREDIVGRREGPKLRAALRAMRDF
                     ARRHLEKASSATKPEGAAAAYLPLALVALYLKRMERADYRPFDTVVEVPQWRRQWAMW
                     RAARRVETGAVKAAKGAHEDE"
     gene            2196..4493
                     /locus_tag="VKU03_14140"
     CDS             2196..4493
                     /locus_tag="VKU03_14140"
                     /inference="COORDINATES: protein motif:HMM:NF025214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HLJ72456.1"
                     /translation="MPAPPPEKLRRAFAAFQKGDLDEAERLSRAVVSAKPDLFDAWHL
                     LAFVQAAMKRSDKAVQSYDRALALRSDIAEVHVNRGAALQDLSRFEEALAAYQKAIAL
                     KSGYPPAHANRGLAFHALGRYDEALAAHDRAIALHAGYADAHANRASTLYELKRYEEA
                     LASYDRALAINSGDVEAQFNRANTLRALERLDAALPAYDRVLAQRPDHVGALANRGAA
                     LHERRRFGEALASFDRLVALRPEDADAHYNRANALHSLGRYDEARAAFDRALALRPDM
                     VDALYNRANTLFVMKSYGSALDGFERVLQRKADHPFALERAASCVLHLCDWDKRPHYE
                     RALAANIRAESAIISPFTLLGYSDDPGLQLQCARHFASTIGGFAPAPRAAPRQPGKWR
                     IAYLSADFQEHPVAYLIAELFELHDRQEFEIFGFSFGRDDGGAMRKRLVKAFDRFEDA
                     SGLSDAEVAARLSALDVDIAVDLMGFTSESRSGALARRPAPIQISYLGYAGAMGAAFI
                     DYILADPVVAPPGSDAFFSEKVVRLPDCYLPNDRQRAIAEETPTREQAGLPPVGFVFA
                     CFNNSWKITPPFFDIWMRLLQAVDGSILWLAPTDTAAQANLRNAAITRGVDPARLIFA
                     ERKGQAEHLARHRLADLFLDTLPYNAHTTASDALWVGLPLITCPGRAFASRVAASLLT
                     AMGLPELIARDASEYEAMALKFAREPALLAAARERLAANRLTTPLFDSRRRTCGIEAA
                     YRTMIERYERGEGAASFDVAGIDED"
     gene            complement(4646..>5692)
                     /gene="trmFO"
                     /locus_tag="VKU03_14145"
     CDS             complement(4646..>5692)
                     /gene="trmFO"
                     /locus_tag="VKU03_14145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441615.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate--tRNA-(uracil(54)-
                     C(5))-methyltransferase (FADH(2)-oxidizing) TrmFO"
                     /protein_id="HLJ72457.1"
                     /translation="IPPDDWDSVIIATGPLTSPGLAEAIRQLTGEDSLAFFDAIAPIV
                     HRDTIDMDKAWLQSRYDKAGPGGSGADYVNCPLARDEYEAFVDALIAGEKTEFKEFEG
                     TPYFDGCLPIEVMAARGRETLRHGPMKPVGLTNAHQPTVKPYAVVQLRQDNALGTLFN
                     MVGFQTKLKYGEQARVFRMIPGLERAEFARLGGLHRNTFLNSPRLLDPMLRLKAMPRL
                     RFAGQITGCEGYVESAAVGLIAGRMAAAERLGAPVTIPPITTAIGALLNHITGGHIET
                     IDAGPRSFQPMNVNFGLFPPIAEPRVDASGKRLRGPERGVARKRALSARAGRDLEAWL
                     RGTSASDERPRAAE"
BASE COUNT          968 a         1807 c         1840 g         1077 t
ORIGIN      
        1 cagaccttcc cgatcttcaa cagggacgtg gtctatgtct ccaacgcgcc gctgaccgac
       61 atccagaagg tgatggaagt cttcaacctc gccgttaccc cggcggcttc agccgccgag
      121 atttactccg tcgtgccgag ataacgagat ttcgcctgtc ggggttgaga ccttcgtttc
      181 gaccgcgact tcgctatcgc ccggctcgac cctcgcgcga gcgcaggcgg gcgacccgca
      241 aagcaggctc gaatcgaaac tcagcacggc gatagcaatc gcgcgcgtga ccgaaggttt
      301 tgattttccg agcttgcctc agctcgctac cgcgagcttc ttcttaatct cgctcttcag
      361 cgccagggcg ccggcggaaa gttcgccatc ggcggccttg agcaagaaat tgtcgaggcc
      421 gccgcgatgc tcgaccgagc gcagcgacga ggcggcgacg cgcaggcgaa ccgaacgcgc
      481 aagcgaatcg gagatcagcg tgacattgca gaggttcggc aagaagcgcg tgcgcgtctt
      541 gcgattggaa tggctgacct tattgccggt ctgcacggcc ttgccggtca attcgcaacg
      601 gcgtgacata gcgcttgtct ttcccttgaa aagtgggatt ttcgagcgaa accagagata
      661 taaacgcctc aaagggccgc ccgaaagcgt cccgcggcct taaatccggg tttctgggtc
      721 gcggggtcta taggggaagg ggcgagaggc gtcaagcaag gcgccgaggc ggcgtcaaaa
      781 ttcggcggga tattgggcat aaatcgattg aactggaggg cgcgccatgc tcgccattcg
      841 tacgccaaat gtcggcgaac tgccggctct cggcgatctt tgcatgcgct caaaggcggt
      901 ctggggctat gacgaaaagt tcatggcggc gtgtcgcacc gaattatcgt tcgacgcttt
      961 cgacttgctt gtcacgcgga tcgccgttgt ggagcaagac aataaactcc tcggcgtcgc
     1021 gcaaattacg ctcgtcgata ccgaggccca tttgatgaaa ctctttgtcg atcctaccgc
     1081 gctccgtcac ggcgccggca aagtcctttt cgcctgggcc gtggcggccg cgaaagatat
     1141 gggcgcgcgc cggctcctca tcgattccga tcccgccgcc gcttcgttct atcgtcgcat
     1201 gggcgcgcgc gatgcgggcc tcgcgccgtc cgggtccatc gccggacgaa tgttgccgaa
     1261 attgctcttc gcgcttggct cggcgacttg aataccggga tgacggacga ggatcggcta
     1321 gaccaggcct acgcccattg cgacgccttg acgcgcgcgc atgaccgcga ccgctggctt
     1381 gcagcgcttt tcgcgccggc ggaagcgcgc cggcatctcc acgcgctcta cgctttctct
     1441 tatgaagtcg gtcggctgcg cgattttgtc cgtgagccga tggccggcga aatccgcttg
     1501 caatggtggc gcgaggcgat cgaaggcgag agacgtaccg aagcgcaagc ctcgcccgtc
     1561 gccgcggcgc ttatcgacac gatcgcgcgc ttccgcctgc cgatccaggc tttcgactcg
     1621 atgctcgcag cgcgcgtttt cgacctctat gacgatccca tgcccgatgt cgccgctttc
     1681 gaggcctatt gcggcgagac gtcctcgctg cttttccgcc tcgcttcctt gatccttgcg
     1741 ggtcgcgaca atggcggcgc cgacgcggcg ggacacgctg gcgtcgcctg tgcgacggcc
     1801 ggcttattgc gcgcgttgcc gatcgccagc atgcgcggcc aagtctatct gccaatggac
     1861 attctgagcg aaaacggcgt gtcgcgtgag gatattgtgg gaaggcgaga ggggcccaag
     1921 ctgcgcgcgg ccctgcgcgc gatgcgggac ttcgctcgcc ggcatctcga aaaagcctcg
     1981 tcggcgacaa aacctgaggg cgccgccgcg gcctatctgc cgcttgcgtt ggttgcgctt
     2041 tacctcaagc gcatggagcg cgccgactat cgtcccttcg atactgtcgt cgaggttccg
     2101 caatggcgcc gtcagtgggc catgtggcgc gccgcgcggc gcgtcgagac aggcgcggtg
     2161 aaagcggcta agggagcgca tgaggacgag taaaaatgcc ggcgccgccg cctgaaaaat
     2221 tgcgacgcgc cttcgcggcc ttccaaaaag gcgacttgga cgaggcggag cggctgagcc
     2281 gcgctgtcgt ctcggcaaag cccgatcttt tcgacgcctg gcatttgttg gccttcgtcc
     2341 aggccgcgat gaagcgcagc gacaaggcgg tgcagagcta tgatcgcgcg ctcgctctgc
     2401 gctccgatat tgcggaggtt cacgtcaatc gcggcgctgc gcttcaagac ctgagccgct
     2461 tcgaagaggc gctcgccgcc taccaaaaag cgatcgcgct gaagagcggc tatccgcccg
     2521 cccacgccaa tcgcggcctt gccttccacg cgctcggccg gtacgacgag gcgcttgccg
     2581 cccatgaccg cgccattgcg ctccatgcgg gctacgccga cgcgcacgcc aatcgcgcct
     2641 cgacgcttta cgaattgaag cgctatgagg aggcgctcgc cagctatgat cgcgctttgg
     2701 cgatcaattc cggcgatgtc gaagcgcagt tcaatcgcgc caacacattg cgcgccttgg
     2761 agcggctgga tgcggccttg ccggcgtatg atcgcgtctt ggcgcagcgg cccgatcatg
     2821 tcggcgcgct ggccaatcgc ggcgcggctt tgcatgaaag gcggcgcttc ggcgaggcgc
     2881 tcgcctcgtt cgatcggctc gtcgcgttgc ggcctgaaga tgcggacgcc cattacaatc
     2941 gcgccaatgc gttgcacagt ctcggccggt acgatgaggc gcgcgctgcg ttcgatcgcg
     3001 ctctggcgct gcggcctgac atggtcgacg cgctctataa tcgcgccaat acgcttttcg
     3061 tcatgaaaag ctacggctcg gcgctggacg ggttcgagcg cgtcctgcaa cgaaaggccg
     3121 atcacccctt tgccctggag cgcgcggcga gttgcgtcct tcatctctgc gattgggaca
     3181 agcggccaca ttacgagcgc gccctggccg cgaacatacg cgcggaaagc gcgatcattt
     3241 cgcccttcac cttgctgggc tacagcgacg atccagggct gcaattgcaa tgcgcgcgtc
     3301 attttgcttc gacgattggc ggattcgcgc cggcgccgcg cgccgcgccg cgtcaacccg
     3361 gcaaatggcg catcgcctat ctctccgccg attttcagga acatccggtc gcctatctca
     3421 tcgcggaatt gttcgaactt cacgatcgcc aggaattcga aattttcggc ttctccttcg
     3481 ggcgcgacga cggcggggcg atgcgtaagc gactcgtcaa ggcgttcgat cgtttcgagg
     3541 acgcgagcgg cctcagcgac gccgaggtcg ccgcgcgtct tagcgcgttg gatgtcgata
     3601 tcgccgtcga cctgatgggc tttacgagcg aatcccggtc gggcgccctg gcgcgccggc
     3661 ctgcgccgat acaaatcagc tatctcggct atgccggcgc gatgggggct gccttcatcg
     3721 attatattct cgccgacccc gtcgtcgcgc cgccgggaag cgacgctttc ttttccgaga
     3781 aggtcgtacg gctgccggat tgttatctgc ccaatgatcg tcagcgcgcg atcgctgaag
     3841 agacgccgac acgggagcag gcgggactgc cgcctgtcgg ctttgtcttt gcctgcttca
     3901 acaatagctg gaagattacg ccgccgttct tcgacatttg gatgcggctg ctgcaggcgg
     3961 ttgacgggtc gattttgtgg cttgcgccga ccgacacggc ggcgcaggcg aatttgcgca
     4021 acgccgccat aacgcgcggc gtcgacccgg cgcgcctgat tttcgcggaa cgaaagggac
     4081 aggccgaaca tcttgcccgc catcgcctgg cggatctgtt cctggatacg ctgccgtata
     4141 acgcgcatac gacggcgagc gatgcgctct gggttgggct gccgttgatc acctgtcccg
     4201 gccgggcatt cgcctcgcgg gtggcggcga gcctcctgac ggcgatggga ttgccggagc
     4261 tgatcgccag agatgcgagc gaatacgaag ccatggcgct gaaattcgct cgcgagccgg
     4321 cgttgctcgc ggcggcgcga gagcggcttg cggcgaaccg gctgacgacg ccgctgttcg
     4381 acagccgacg ccgtacctgc gggatcgaag ccgcatatcg gaccatgatc gagcggtacg
     4441 agcgggggga gggggcggcg agctttgatg tggcgggtat cgatgaggat taggcatttg
     4501 cgatgtggct ttatggcttt catggctttt gctacgcaaa gaccagggat ggcgaacggc
     4561 ggtcaaggcg cgcgaagcgc ccggctaatc cggcatggcc ttgacggcga gcaaggctca
     4621 ggcgccgcgg cctcgaaaat tgtcgctact ccgccgcgcg cggccgctca tcgctcgccg
     4681 acgtaccccg caaccatgcc tcgagatccc gccccgcccg cgccgacagc gctcgcttgc
     4741 gagcaacgcc gcgctccggc ccgcgcagcc tcttgccgct cgcatcgacg cgcggctccg
     4801 cgatcggcgg aaacagcccg aaattcacgt tcatcggctg aaacgagcgc ggcccggcgt
     4861 cgatcgtttc gatatggccg ccggtgatgt gattgagcag cgcgccgatc gcggtcgtga
     4921 tcggcggaat cgtcaccggc gcgccgagcc gctcggctgc cgccatccgc ccggcgatca
     4981 gtccgaccgc ggcgctttcg acatagcctt cgcagccggt gatctggccg gcgaagcgca
     5041 aacgcggcat cgccttcagc cgcagcattg gatcgagcag gcgcggcgaa ttgaggaacg
     5101 tgttgcgatg gagcccgccg agccgcgcga attcggcgcg ctcgaggccg gggatcatcc
     5161 ggaagacgcg cgcctgctcg ccatatttca gcttggtctg gaacccgacc atattgaaca
     5221 aagtcccgag cgcgttgtcc tgtcgcaatt gcacgacggc gtaaggcttg acggtcggtt
     5281 gatgcgcatt ggtgagaccg accggcttca tcggaccgtg gcgcaaggtt tcccgccccc
     5341 gcgccgccat cacttcgatc ggcaggcagc cgtcgaaata gggcgtgcct tcgaattcct
     5401 tgaactccgt cttctcgccg gcgatgagcg cgtcgacaaa ggcttcgtat tcatcgcggg
     5461 ccagcggaca attgacgtaa tcggcgcccg agccgccggg accggccttg tcgtagcgcg
     5521 actgcagcca ggccttgtcc atatcgatcg tgtcgcgatg gacgatcggc gcgatcgcgt
     5581 cgaagaaggc gagtgaatct tcgccggtga gctggcggat cgcttcggcg aggccaggcg
     5641 aggtcagcgg gcccgtcgcg atgatgacgc tgtcccaatc gtcgggcgga at
//
LOCUS       DATGJW010000469         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_608676, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000469 DATGJW010000000
VERSION     DATGJW010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_608676"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..631)
                     /locus_tag="VKU03_14150"
     CDS             complement(<1..631)
                     /locus_tag="VKU03_14150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TGS domain-containing protein"
                     /protein_id="HLJ72458.1"
                     /translation="MINITFKDGASRAFEPGLSGLDIAKTISPSLAKRTVAMILDGRL
                     SDLSDRVTQDATVEFISRDDPRALELIRHDGAHVLAEAVQSLWPGTQVTIGPVIENGF
                     YYDFFRNEPFTPEDFPAIEKRMREIIAADKPFTKEVWRRDQARDFFEKKGEAFKVELV
                     DTIPPGEDLKIYRQGEWLDLCRGPHMTSTGKVGDAFKLMKVAGAYWRGDS"
     gene            823..1227
                     /locus_tag="VKU03_14155"
     CDS             823..1227
                     /locus_tag="VKU03_14155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011157549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLJ72459.1"
                     /translation="MLTRRGFATCALCAITGFAATAAEAQSAAASGFKRTILQRVDGP
                     SPGYETVMVAVEIEPGVPVAKHTHPGVESSFVIEGETELSVEGQAPRTIAAGESFQIP
                     AGVPHSGKNGAKPTRIAAVYVVEKGKPLASPA"
     gene            complement(1185..1538)
                     /gene="yidD"
                     /locus_tag="VKU03_14160"
     CDS             complement(1185..1538)
                     /gene="yidD"
                     /locus_tag="VKU03_14160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009489450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="membrane protein insertion efficiency factor
                     YidD"
                     /protein_id="HLJ72460.1"
                     /translation="MSALRMAPRRVANGAIRVYQLTLSSILGRQCRYLPTCSEYVDEA
                     IRRHGVWAGGWMGVARICRCHPLGGAGFDPPPAATPERAHWLTPWRYGAWRTPPAEMK
                     SAQAGDASGLPFSTT"
     gene            complement(1535..1990)
                     /locus_tag="VKU03_14165"
     CDS             complement(1535..1990)
                     /locus_tag="VKU03_14165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="iron-sulfur cluster assembly scaffold protein"
                     /protein_id="HLJ72461.1"
                     /translation="MLDSIYNRRILELAGDIPRLGRLAAPDATARAHSKLCGSTVIVD
                     LCVKDGRVSEFAHDVRACALGQASSSLMARHIIGATPQELIALRDQMRRMLKEGGPPP
                     SGKWADFAALEPVRDYKARHASTLLTFEAVADALAQIEARQAEIAAPAK"
BASE COUNT          367 a          637 c          673 g          354 t
ORIGIN      
        1 tggaatcgcc gcgccaatag gcgccggcga ccttcatcag cttgaacgcg tcgccgacct
       61 tgccggtcga agtcatgtga gggccgcggc agagatcgag ccactcgccc tggcggtaga
      121 ttttcaggtc ctcgccgggc ggaatcgtat cgaccaactc aaccttgaag gcctcgccct
      181 tcttctcgaa gaaatcgcgc gcctgatcgc ggcgccagac ctccttggtg aagggcttgt
      241 cggcggcgat gatctcgcgc atgcgctttt cgatcgccgg aaaatcctcc ggcgtgaacg
      301 gctcgttgcg gaagaaatcg taatagaacc cgttctcgat caccgggccg atggtcacct
      361 gcgttcccgg ccagagcgac tgtaccgcct cggcaaggac gtgggcgccg tcatggcgga
      421 tgagctccaa agcgcgcggg tcgtcgcgcg aaatgaactc gacggtcgcg tcttgcgtca
      481 ccctgtcgga gaggtcgctc agcctcccgt cgaggatcat cgcgacggtt cgtttggcga
      541 gcgacgggga gatggttttg gcgatgtcga ggccggaaag gccgggctca aaagcgcgcg
      601 aggcgccgtc cttgaaagtg atattgatca tgaccgctcc ttgctcactc gccgacacca
      661 atcggcgtaa gcatcaggcg cgatatagcg ggcgagagag gcgccggcaa tgggcgcgcc
      721 aggcgtcgtc gcgggatcgc ggacccgctt caattacgcc ttgtcggcga tttttccttt
      781 tgacagaatt gcccggtcag cagcatgtgg ggagggatcg tcatgttgac ccgtcgtggc
      841 tttgcgactt gcgcgctttg cgcgatcacc ggcttcgccg ccaccgcggc ggaggctcaa
      901 tcggcggcgg ccagcggttt caagcgcacc atcctgcagc gcgtggacgg accgtctccg
      961 ggctacgaga cggtcatggt ggcggtcgag atcgaacccg gcgtcccggt cgccaagcat
     1021 acgcatccgg gcgtcgaatc cagcttcgtc atcgagggcg agacggaatt gtcggtcgaa
     1081 ggacaggcgc ccaggaccat cgccgccggc gaaagcttcc agattccggc cggcgttccc
     1141 catagcggca agaacggcgc gaagccgacg cggatcgccg ccgtctatgt ggttgagaaa
     1201 ggcaagccgc tcgcatcgcc ggcttgagcg gatttcatct cggccggcgg cgtccgccag
     1261 gcgccatagc gccacggcgt tagccaatgc gcgcgctccg gcgtcgccgc cggcggcgga
     1321 tcaaaaccgg cgccgcccag cggatggcaa cggcagattc tcgcgacgcc catccagcca
     1381 cccgcccaga cgccgtggcg gcggatcgct tcgtcgacat attcggagca ggtcgggaga
     1441 taccggcatt gccggccgag aatggaggaa agagtcagtt gatagacgcg gatcgcgcca
     1501 ttcgccacgc gacgtggggc catgcggagc gcgctcattt tgccggcgcc gcgatttcgg
     1561 cttggcgggc ttcgatctgc gccagcgcgt cggcgaccgc ttcgaaggtc agcaaggtcg
     1621 aggcgtggcg cgccttgtag tcgcggaccg gctcgagcgc ggcgaaatcg gcccacttgc
     1681 cggaaggcgg cggcccgcct tctttcagca tgcgccgcat ctgatcgcgc agcgcgatca
     1741 actcttgcgg agtcgcgccg ataatgtgtc gcgccataag cgaggacgac gcctggccca
     1801 acgcacaagc gcgcacgtca tgagcgaatt cgctgacccg gccgtccttg acgcataggt
     1861 cgacaatcac ggtcgaaccg cagagtttgg aatgcgctcg cgcagtcgcg tcgggcgcgg
     1921 cgagccggcc gagacgcgga atgtcgccag ccaattccaa aattcgcctg ttataaatac
     1981 tatcaagcat atgtgacttg gaaaatcgcg agggatcagg tggcgcatgt t
//
LOCUS       DATGJW010000470         4238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_300670, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000470 DATGJW010000000
VERSION     DATGJW010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4238
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_300670"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..167
                     /locus_tag="VKU03_14170"
     CDS             <1..167
                     /locus_tag="VKU03_14170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007876417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042586 - peptide deformylase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="peptide deformylase"
                     /protein_id="HLJ72462.1"
                     /translation="AREIEAEGLLATALQHEIDHLNGVLFIDHISKLKRDRVIKKFVK
                     QARQKESERA"
     gene            305..1072
                     /locus_tag="VKU03_14175"
     CDS             305..1072
                     /locus_tag="VKU03_14175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011923524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine amidotransferase"
                     /protein_id="HLJ72463.1"
                     /translation="MVTRNGSPKILVLLHQEHSTPGRIGRLLRAQGAELDIRRHSLDE
                     PLPEHLADHDGVVVFGGPMSANDDCAWICRQIGWLTTPLKEDKPFLGICLGAQMLTRA
                     LGARVFTYPDQRAEIGYAPIRPTAAADRLCAAPFPRWVYQWHRDGFDLPSGAELLAEG
                     GEAFPIQAYRYGGKAVALQFHPEVTYQMICRWTVRGHERLSLPGAQPRESHLDGWRQF
                     DGAVERWLHAFLPPWLEGRMPRAAAPAPAQEKALEFA"
     gene            complement(1129..2697)
                     /gene="xseA"
                     /locus_tag="VKU03_14180"
     CDS             complement(1129..2697)
                     /gene="xseA"
                     /locus_tag="VKU03_14180"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HLJ72464.1"
                     /translation="MPEIEPASTNLPEISVSELSSALKRVVEDRFAYVRVRGEISNYR
                     GPHASGHVYFCLKDQSARIDAVIWRSTFARMRVKPEEGLEVIAAGKVTTYPGKSSYQI
                     VVESLEPAGIGALMALLETRRVKLAAEGLFDASRKRLLPYLPRVIGVVTSPTGAVIRD
                     ILHRLADRFPAHVLVWPVRVQGETSALEIADAIAGFNALSAGGAIPRPDVLIVARGGG
                     SLEDLWGFNEEIVVRAAAASAIPLVSAVGHETDTTLIDHAADRRAPTPTAAAELATPV
                     RAELLAATADLGGRLSGAALRLGERRRAELRAIARALPSGEALLAIPRQRLDRAAGAL
                     RAAARADGHRRHIALERAARRLGAQSPYARMARAHERLESFGARLVRAGRIDRERRRE
                     RLQRLEARLAAALAARAQLAAHQAARFRERAAAAQGRLRRAWRSGLDRRRAALASLDQ
                     FLTALSYRNVLKRGFALVRDEAGAPLRGSAAISPGQPLRIEFADGEIAARAQGEAPPR
                     RRPAKTANDQGSLF"
     gene            2900..4138
                     /locus_tag="VKU03_14185"
     CDS             2900..4138
                     /locus_tag="VKU03_14185"
                     /inference="COORDINATES: protein motif:HMM:NF022515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2865 domain-containing protein"
                     /protein_id="HLJ72465.1"
                     /translation="MTNETRGGARRRRARTAWAAAAAALLALAAGDAGAQDAQYCQQL
                     RAQIAAAGQTRSGPAAAYDAAAQKQKAEIDKTIAYSRQIGCENRKFLFFGKDPPAQCG
                     ALYAQIARMQANLGQLQARGSGGGGEARAQLVARYQSECEQTRPRGFFEALFGNPREE
                     PSQRVTDVPLVPDPNAPGQPGDNTNPTDGVQAHAGSKEVCVRTCDGYFFPIGYSGSGG
                     HSGDLQEMCRALCPNAETALYSYSPAGDISQAVGADGTRYADLPNALKYRKSLDPTCT
                     CRRRGQSWAQALADAEALLGERKTDIIVTQQKSDEMSRARPDPKAKPSKAQAAEAAKN
                     PPPPADAKNPPDAGTQDPNNPTDALLGEQPTPVSRENSGIASGDAVGGPNYSKAQGQV
                     EEVAGPDGVKRRVRIIDPTL"
     gene            complement(4101..>4238)
                     /locus_tag="VKU03_14190"
     CDS             complement(4101..>4238)
                     /locus_tag="VKU03_14190"
                     /EC_number="6.1.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386574.1"
                     /note="charges one glutamine molecule and pairs it to its
                     corresponding RNA trinucleotide during protein
                     translation; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate--tRNA ligase"
                     /protein_id="HLJ72466.1"
                     /translation="GRKGRALYHPLRLALTGREAGPELANLLPLIGRAKALDRLSARA
                     A"
BASE COUNT          717 a         1481 c         1465 g          575 t
ORIGIN      
        1 aggcgcggga gatcgaggcc gaaggtctgc tcgccaccgc cctccagcac gagatcgatc
       61 acctgaacgg cgtgttgttc atcgaccaca tttcgaagct caagcgcgac cgcgtgatca
      121 agaaattcgt caagcaggcc aggcagaagg agagcgagag ggcttaagct catcgggccc
      181 ggcaaggccg ccatgcattg agccgaaatc ctggtcagat cggcgcaacc tttgctatct
      241 accgtcatag atcggcggca ttggagaatc ggctcgcgat tcggcagggt taggtctcga
      301 cgggatggtc acgcgcaacg gaagcccgaa aatccttgtt ctccttcatc aggagcattc
      361 gacccccggt cgcatcggcc gattgctccg cgcccagggc gctgaactcg acatccgccg
      421 ccattccctc gacgaaccgc tgcccgagca tctggccgac catgacggcg tcgtcgtgtt
      481 cggcggcccg atgagcgcca atgacgattg cgcctggatt tgccgccaga tcggctggct
      541 gacgaccccg ctcaaggagg acaagccctt tctcggcatc tgcctcggcg cgcagatgct
      601 gacccgggcg ctgggcgccc gcgtcttcac ctatcccgat cagcgcgccg agatcggcta
      661 tgcgccgatc cgcccgaccg ccgccgccga ccgcctctgc gccgcgccgt tcccgcgctg
      721 ggtctatcaa tggcaccgcg acggattcga tcttccctca ggcgccgaat tgctcgcgga
      781 aggcggcgaa gccttcccca ttcaggccta ccgctatggc ggcaaggcgg tggcgctgca
      841 attccacccc gaagtgacct atcagatgat ctgccgctgg accgtgcgcg gccacgagcg
      901 cctgagcctg cccggcgcgc agccgcggga aagccatctc gacggctggc gccaattcga
      961 cggcgcggtc gagcgctggc tccacgcgtt cctgccgcca tggctggagg gccgcatgcc
     1021 ccgggcggcg gcgcccgcgc cggcgcagga aaaagcgctg gaattcgcgt agcggcggcg
     1081 ctgcagccgc ggtcggccgc ggcggcacaa caggcgcgcc gccaagactc aaaacaaact
     1141 gccctggtcg ttcgccgtct tggccggccg cctgcgcggc ggcgcttcgc cttgggcgcg
     1201 cgcggcgatc tccccgtcgg cgaattcgat gcgcagcggc tggccggggc ttatcgccgc
     1261 agagccgcgc aacggggcgc cggcctcgtc gcgcaccagg gcgaagccgc gcttcaacac
     1321 gttgcgatag ctcagcgcgg tcaggaactg atcgagcgaa gccagtgcgg cgcggcgccg
     1381 gtcgagcccg ctgcgccagg cccgccgcaa ccggccttgc gcggccgcgg cgcgttcgcg
     1441 aaagcgcgcc gcctgatgcg ccgccaattg cgcgcgggcg gcaagcgcgg cggcgagccg
     1501 cgcttcgagg cgctgcaggc gctcgcgccg ccgctcgcgg tcgatcctgc cggcgcggac
     1561 gaggcgggcg ccgaagcttt ccagccgctc gtgcgcgcgg gccatgcggg cgtagggcga
     1621 ttgcgcgccg agccggcgcg ccgcgcgctc cagcgcgatg tggcggcgat ggccgtcggc
     1681 ccgcgccgcg gcgcgcagcg cgccggcggc gcgatcgagc ctctggcgcg ggatcgccag
     1741 caaggcctcg cccgagggca gcgcccgggc gatcgcgcgc aattcggcgc ggcggcgctc
     1801 gccgagcctg agcgccgcgc cgctcagccg gccgccgaga tcggcggtcg cagccagcaa
     1861 ttcggcgcga accggcgtcg ccagctcggc cgccgcggtc ggcgtcggcg cgcgccgatc
     1921 ggcggcgtga tcgatcaggg tcgtgtcggt ctcgtggccg accgcggaaa cgagcgggat
     1981 ggcgctcgcg gccgcggcgc ggacgacgat ttcctcgttg aagccccaga gatcttcgag
     2041 cgagccgccg ccgcgcgcga cgatgaggac gtccggccgc ggaattgcgc cgccggcgct
     2101 cagcgcattg aagccggcga tcgcgtcggc gatttccagg gccgaggttt cgccctgaac
     2161 cctgaccggc cagaccagga catgggctgg aaagcggtcg gcgaggcggt gcagaatgtc
     2221 gcgaatcacc gcgccggtcg gcgaggtgac gacgccgatg acgcgcggaa gataaggcag
     2281 aagccgcttg cgcgaggcgt cgaacaggcc ctcggcggcg agcttgacgc gccttgtttc
     2341 gagcaacgcc atcagcgcgc cgattccggc cggctccagc gattcgacga cgatctgata
     2401 agaggacttg ccgggatagg tggtgacctt gccggcggcg atcacctcca gcccttcctc
     2461 cggcttcacg cgcatccgcg cgaacgtgct tcgccagatc accgcatcga tccgagcgct
     2521 ttgatctttc aagcaaaaat agacatggcc ggaggcgtgc gggccgcgat aattggagat
     2581 ttcgccgcgc acccggacat aggcgaaacg gtcctcgacg acgcgcttca acgcgctcga
     2641 aagctcgctg accgaaattt cggggagatt ggtcgaggcc ggctcgattt cgggcatggg
     2701 tctatttgcc cgatcggccg cgccagcgcc agccgcccgc gacgcgctgg ctgcggccgg
     2761 ggaaagcggg taagaaggcg gcgttccggc cgaagcggcc atgttgaggc cagattgtcg
     2821 cgcgaagacg aggccattcg ccgaacggaa agagcgattc gatcgccgca gcggcagcat
     2881 tggatgagac gatccgccga tgacgaatga gacaaggggc ggcgcgcggc gtcggcgcgc
     2941 gagaaccgct tgggcggccg cggcggctgc gcttttggcg ctggcggccg gcgacgccgg
     3001 cgcgcaagac gcgcaatatt gccagcaatt gcgggcgcaa atcgccgccg caggccagac
     3061 gcggtccggc ccggccgcgg cctatgacgc ggcggcgcaa aagcagaagg ccgaaatcga
     3121 caagaccatc gcctatagcc gccagatcgg ttgcgaaaac cgcaaattcc tattcttcgg
     3181 caaggatccg ccagcccaat gcggggcgct ttacgcccag atcgcccgca tgcaggccaa
     3241 tctcggccag ctgcaggcgc gcggctcagg cgggggcggc gaggcccggg cccaactcgt
     3301 cgcgcgctac cagtcggaat gcgagcaaac gcggccccgc ggcttcttcg aggctttatt
     3361 cggcaatccg cgcgaggagc cgtcgcagcg cgtcaccgac gtgccgctcg tccccgatcc
     3421 caacgccccg ggccaacccg gcgacaacac aaatccgacc gacggcgtcc aagcccatgc
     3481 cggctccaag gaagtgtgcg tgcgcacctg cgacggctat ttcttcccga tcggctattc
     3541 gggcagcggc ggccattccg gcgatctcca ggaaatgtgc cgggcgctgt gccccaatgc
     3601 ggagaccgcg ctctacagtt attcgccggc cggcgacatc agccaggcgg tcggcgccga
     3661 cggcacgcgc tacgccgacc tccccaacgc gctcaaatac cgcaagagcc tcgatccgac
     3721 ctgcacttgc cggcgccgcg gccagagctg ggcgcaagcc ttggccgacg ccgaggcgct
     3781 gctcggcgag cgcaagaccg atattatcgt aacgcagcag aaatcggacg aaatgtcgcg
     3841 cgcgcggccc gatccgaagg ccaagccgag caaggcgcaa gcggcggaag ccgccaagaa
     3901 tccgccgccg cccgccgacg cgaagaaccc gcccgacgcc gggacgcaag acccgaacaa
     3961 tccgaccgac gccctgctcg gcgagcagcc gaccccggtc agccgcgaga attccggcat
     4021 cgcctcgggc gacgcggtcg gcgggccgaa ctattcgaag gcgcagggcc aggtcgagga
     4081 agtcgccggc ccggacggcg tcaagcggcg cgtgcggata atcgatccaa cgctttagcc
     4141 cggccgatca acgggaggag atttgcgagc tccgggcccg cctcgcggcc ggttaaggcg
     4201 aggcgcagcg gatgataaag ggcgcggccc ttgcgtcc
//
LOCUS       DATGJW010000471         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_91999, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000471 DATGJW010000000
VERSION     DATGJW010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91999"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(140..1033)
                     /gene="tsf"
                     /locus_tag="VKU03_14195"
     CDS             complement(140..1033)
                     /gene="tsf"
                     /locus_tag="VKU03_14195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892610.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HLJ72467.1"
                     /translation="MANVTAALVKELRERTGAGMMDCKNALTETDADIEAAVDWLRKK
                     GLAKAAKKSGRVAADGLVSVAVEANFGVVVEVNSETDFVARNDDFQALVRGITATAID
                     KGPGVDADTIRDAHFPGGGTVSEAVANAIATIGENITLRRAAGLHVPHGAIGRYVHGA
                     VVDGLGRIGVIVALESKGAAEPLAALGRQVAMHIAAANPLALDPSGLDPATVEREKSV
                     LREKNAGKPAHVLEKIVESGLKTYYKEVCLLDQSYVHDQAKTVAQAVKEAEKNVGAPI
                     AVKGFVRYALGEGIEKPAEEG"
     gene            complement(1121..2164)
                     /locus_tag="VKU03_14200"
     CDS             complement(1121..2164)
                     /locus_tag="VKU03_14200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590711.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HLJ72468.1"
                     /translation="MSLPDFSMRQLLEAGAHFGHQSHRWNPKMAPFIFGARNNIHIID
                     LAQTVPLLHQALKAVSDTVAKGGRVLFVGTKRQASDAIAEAAKRSAQYYVNARWLGGM
                     LTNWKTISGSISRLRKLDEMLAAGAVGLTKKERLMLSRERDKLEKALGGIKDMGGTPD
                     LVFVIDTNKEQLAIKEANRLKIPVAAIVDTNCDPDGIAYPVPGNDDAGRAIQLYCDLV
                     ARAAIDGISRGQGALGVDLGAAETPIAESLPEPAAAAAAEPEAPAEQFELLAAPRGAP
                     DDLAKLTGVGPQIVKKLNDAGVFHYWQIGALTQAEAEKLDADLKLNGRILRDHWIEQA
                     RALTAAPLEEAEV"
     gene            2351..>2641
                     /locus_tag="VKU03_14205"
     CDS             2351..>2641
                     /locus_tag="VKU03_14205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72469.1"
                     /translation="MRLQAAAGLSVALAQKDRGQISSPVNHAHDIDRKGSYPVKNNRW
                     VDGGSNETQAIRRPPTSFSATAIGVRRRSGEFSPRPTSRRLIGLDAYSPLSGC"
BASE COUNT          409 a          867 c          884 g          481 t
ORIGIN      
        1 tctcgctgat ggtgacggga gagaacgcaa acaggcgacg caaccttatt cgctcgccgc
       61 gtctttcgca tccgcccggc ggcgccggcc gcatgcaaaa agggcggctc gaaagccgcc
      121 ctttgaattt ccgtagcgct cagccttctt cggccggttt ttcgattcct tcgccgagcg
      181 cgtagcgcac gaagcccttg accgcgatcg gcgcgccgac gttcttctcg gcctccttca
      241 ccgcctgggc gaccgtcttg gcctggtcgt ggacatagga ctgatcgagg agacagacct
      301 ccttgtaata ggtcttcagg ccggattcga cgatcttctc aagaacatgg gccggcttgc
      361 cggcgttctt ctcgcgcagc accgatttct cgcgttcgac cgtcgccggg tcgaggcctg
      421 aaggatcgag cgccagcggg ttcgcggcgg cgatatgcat cgcgacctgg cggccgagcg
      481 cggcgagcgg ttcggccgcg cccttcgatt ccaaggcgac gatgacgccg atccggccga
      541 ggccgtcgac caccgcgcca tgcacatagc gcccgatcgc gccgtgcggc acgtgcaggc
      601 cggcggcgcg gcgcagcgtg atgttctcgc cgatcgtggc gatcgcgttg gcgacggctt
      661 cgctcaccgt gccgccgccg gggaaatgcg cgtcgcggat cgtatcggcg tcgacgcccg
      721 gccccttgtc gatcgccgtc gcggtgatgc cgcgcaccag cgcctggaaa tcgtcgttgc
      781 gggcgacgaa atcggtctcg gaattgacct cgacgacgac gccgaaattg gcctcgaccg
      841 cgaccgagac cagcccgtcg gcggcgacgc ggccgctctt cttggccgcc ttggcgaggc
      901 ctttcttgcg cagccaatcg accgcggcct cgatgtcggc atcggtctcg gtcagcgcgt
      961 tcttgcaatc catcattccg gcgccggtgc gctcgcgaag ctccttcacc agcgccgccg
     1021 tgacattggc catggccgtt cctatcgttt tcaagagagc gggcgcgtcg gcgcccttta
     1081 aaggaggcga cgcccggccg ggaaagccgg gcggcgaaga tcagacctcg gcctcttcca
     1141 gaggcgcggc ggtcaaggcg cgcgcctgct cgatccagtg gtcgcgcaga atgcggccgt
     1201 tgagcttcaa atcggcgtcg agcttctcgg cttccgcctg ggtcagcgcc ccgatctgcc
     1261 aatagtggaa gacgccggcg tcgttgagct tcttgacgat ttgcgggccg acgccggtca
     1321 gcttggcaag atcgtcgggc gcgccgcgcg gggcggcgag gagttcgaac tgctcggcgg
     1381 gcgcctccgg ctccgccgcc gcggcggcgg ccggctccgg caggctttcc gcgatcggcg
     1441 tctcggccgc gccgagatcg accccgagcg cgccctggcc gcgcgagatg ccgtcgatcg
     1501 ccgcccgggc gacgaggtcg caatagagct ggatcgcccg gccggcgtcg tcattgccgg
     1561 gcaccggata ggcgatgccg tcgggatcgc aattggtgtc gacgatcgcc gcgaccggta
     1621 ttttcagccg gttggcctcc ttgatcgcca attgctcctt gttcgtgtcg atcacgaaga
     1681 cgagatcggg cgtgccgccc atatccttga tgccgcccag agccttttcg agcttgtcgc
     1741 gctcgcgcga cagcatcagg cgctccttct tggtcaggcc gaccgcgccg gcggcgagca
     1801 tctcgtcgag cttgcgcagg cgcgagatcg agccggaaat cgtcttccaa ttggtcagca
     1861 tgccgccgag ccatcgggcg ttgacgtaat attgcgccga gcgcttggcc gcctcggcga
     1921 tcgcgtccga ggcctggcgc ttggtgccga cgaacaacac ccgcccgccc ttggcgaccg
     1981 tgtccgacac cgccttcaag gcctggtgca gcagcggcac ggtctgggcg aggtcgatga
     2041 tgtggatgtt gttgcgcgcg ccgaaaatga acggcgccat tttcgggttc cagcgatgcg
     2101 actgatggcc gaaatgggcg cccgcttcga gcaattggcg catggagaaa tcaggcagag
     2161 acatgccttt gaagtccttt ccggttaggc ctcggcgggt tgagaaggag ggttcagcct
     2221 cccaccggag cgacggcttg aagcagccgg cgcgaaaacc cgcctgtggg atgggcgggc
     2281 atataaggga gggagggagg gaaggcaagc gcggcgggag cgacctcctg tattcggcca
     2341 tggcggccgc gtgcgacttc aggcggcggc cgggttatct gtcgcccttg cgcagaagga
     2401 tcgcgggcaa atttcgtcgc ccgtcaacca cgcgcacgat atcgatcgaa agggctccta
     2461 cccggtaaaa aacaaccgtt gggtggacgg cggatcaaac gagacgcagg ctatccgtcg
     2521 tcccccgact tctttttcgg ctaccgcgat cggggtccga cgacgatctg gcgaattttc
     2581 ccctcgcccg acaagccgtc gactgatcgg gcttgatgcg tattcccctt tgtccggctg
     2641 c
//
LOCUS       DATGJW010000472         6181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_92186, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000472 DATGJW010000000
VERSION     DATGJW010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6181
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92186"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            228..2525
                     /locus_tag="VKU03_14210"
     CDS             228..2525
                     /locus_tag="VKU03_14210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532625.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLJ72470.1"
                     /translation="MKFGVGQAVKRVEDPRLITGRGHYTSDYWPEGTLHAAFLRSPHA
                     HARFSFSDLEAARGMPGVKAVYTLDDFAALGPLPCLAPLPNSDKSQTPLKPCPVMADG
                     EAFHVGDIVAMAVAESEREARDAVEAIGVDWEPLEAVIDMEAAIADGAPLLFAEAPGN
                     TAYDTHLGDPDKTKAVFAKAKHVVSLKVVNNRVIANFMETRGAIGEYDPGAKRYFLNV
                     GSQGVHGFRDSIANEVLKIPPDSLRVVTYDVGGGFGTKSILHREYPLVLEAAKRLGRP
                     VRWVADRFEHFVGDTQGRDNVTTAEMALDEKGKFLALRVDILGNLGAYLSQFGPYIPY
                     LGASMATGAYDIPVLHARVRGIYTHTTPVDAYRGAGRPEAAYVLERLVDLCARKLSMP
                     REEIRALNFVKPSQMPYHTQTDRTYDVGDFEGAMRASLAKADYAGFDKRAEAAKARGK
                     LRGIGFASYIECTAWNDNEEGSVELGPDGNFTVLIGTQSNGQGHETAYAQVVSQYLDV
                     PLERIKVVQGDTDRVKTGFGTGGSRSIPIGAVMVGKASEKLVESLKEIAADKLEAAPA
                     DLEVADGRIRIAGTDRSISYEEIAKLPGATPEKLKAGGEYAPADATYPNGTHTCEVEI
                     DPETGVVTIERYTVVDDFGFTLNPLLLAGQVHGGIAQGIGQALHERAVFSEEGQLLTA
                     SFMDYCMPRADDLPNFHFETRNIPSTTNPLGLKGAGEAGSIGSSPAVMNAVSDALWRR
                     YGEHRIDMPATPFAIFRAIQGAANT"
     gene            2642..3076
                     /locus_tag="VKU03_14215"
     CDS             2642..3076
                     /locus_tag="VKU03_14215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013502148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLJ72471.1"
                     /translation="MKRTLIIVATLALGVGAVYAASDAIAKRQALMKANGKAATAIVK
                     IMKGAPFDLAVVQASLKTFIDAAEKGPALFPDDSKTGDNTEALPAIWENKKDFEAHFA
                     KLGDDAKAALGAIKDEASLKAEMPKVFDDCGGCHEKYRAKKQ"
     gene            3239..4153
                     /locus_tag="VKU03_14220"
     CDS             3239..4153
                     /locus_tag="VKU03_14220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012497308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c"
                     /protein_id="HLJ72472.1"
                     /translation="MRRWLLLLLLVAVAAAIAAWFVSAPRPLYARADWRAYEDGGDPG
                     RGRIIFLAGGCESCHMTPGENDPLKLGGGYELKTPFGSFFPPNISPDPQDGIGGWKVV
                     DLANALLVGVSPEPAHLYPALPYTSYSHMKFEDVRDLMAYLRTLPPVKGRAPPHRLSF
                     PFNIRRGVGFWKLLYLKGGVPADDPAQSAAWNRGRYLVEGPGHCAECHSPRDIFGGIV
                     AAKRFSGGLTADGKHKVPNITQGGALAKWSQSDIETALSLGLTPDGDSLGGDMAPVVR
                     NLSQLPKDDIAAIAAYIKSLPAIPSAGK"
     gene            complement(4161..4589)
                     /locus_tag="VKU03_14225"
     CDS             complement(4161..4589)
                     /locus_tag="VKU03_14225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SufE family protein"
                     /protein_id="HLJ72473.1"
                     /translation="MAGIDEIRSDFSILDDWEDRYRYVIDLGRTLEPLSEAAHNDANK
                     VRGCVSQVWLEMTRAAGPDGEPVLIFRGDSDSHLVRGLVAIAIALFSGRTARKILATD
                     ALALFRELGLEQHLTPQRSNGVRAMIERIRADAAAAVQSA"
     gene            complement(4689..5030)
                     /locus_tag="VKU03_14230"
     CDS             complement(4689..5030)
                     /locus_tag="VKU03_14230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72474.1"
                     /translation="MMFLLRSAFWLCLVFSWMPSERTEMARVLGDAKTSLADHAEAAA
                     KAGCAGSAVACGAVLLAADRLATAPAERSAPKAGKDAKAAAKGLRPSANSLTASDRAP
                     RWRGRLLKSGA"
     gene            5325..>6181
                     /locus_tag="VKU03_14235"
     CDS             5325..>6181
                     /locus_tag="VKU03_14235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLJ72475.1"
                     /translation="MTSFIDLLSRRLSALPRLYAQRASGASFIYARLAASVAALILTP
                     LYLAAFGAPDAWAICALVWLILPLAAIVHVARTGKTVEAQSVSLASLLILAAIVALGA
                     RGSHDLIFAWLLLAPLEAAASGSAALLVAACFFVATLLAGLVGAEYWGLVPAGGALPG
                     PAAAALAAPAALYGLVLSFAALALHRRRADVEADHNENYRVLADAIGDLVLRHDQSGA
                     VISASAAAEPLFQTPPQDLLGRGFFERVHVADRPAYLQALDDAQQRDGAVATTLRLRV
                     GGGDEPVFA"
BASE COUNT         1042 a         2063 c         2007 g         1069 t
ORIGIN      
        1 ttgaaatccg gggcgtcctg catggcggcg gtccattggt taaccaaatg cttcaattgt
       61 gcttcagtcc gattaaggaa gcgttgacca aaccgggccg caatgtggcg gccgcgccgt
      121 ccgctggcgt cgcggcgctg acatgctaaa gtgaaagctc ttgaagccgc tggccgccgt
      181 cgccggggcg atccagccca ttcccagagg acttattaat gaagccgatg aaatttgggg
      241 tcggacaggc ggtgaagcgg gtcgaggacc cgcgtctgat caccggccgc ggccattaca
      301 cgtccgatta ttggccggaa gggacgcttc acgccgcttt cctgcgtagc ccgcacgccc
      361 acgccagatt ctccttcagc gaccttgagg cggcgcgcgg catgcccggc gtgaaggccg
      421 tctacacgct cgacgatttc gccgccctcg ggccgctgcc ctgtctggcg ccgctgccca
      481 attcggacaa gtcgcagacg ccgctgaagc cctgcccagt gatggctgac ggcgaagcct
      541 tccatgtcgg cgacatcgtc gccatggcgg tggccgagag cgagcgcgag gcgcgcgatg
      601 cggtcgaggc gatcggcgtc gattgggagc cgctcgaagc ggtgatcgac atggaggcgg
      661 cgatcgccga cggcgcgccg cttcttttcg ctgaagcgcc cggcaacacc gcctacgaca
      721 cgcatttggg cgatccggac aagaccaaag cggtcttcgc caaggccaag catgtcgtca
      781 gcctcaaagt cgtcaacaat cgcgtcatcg ccaatttcat ggagactcgc ggcgcgatcg
      841 gcgagtacga tccgggcgcc aagcgctact tcctcaatgt cggcagccag ggcgtgcacg
      901 gctttcgcga ttcaatcgcc aacgaggttt tgaaaatccc gcccgactcg ctccgcgtcg
      961 tcacctacga tgtcggcggc ggcttcggca cgaagagcat ccttcaccgc gaatatccgc
     1021 tcgtcctcga agcggcgaag cggttggggc ggccagtgcg gtgggtcgcc gaccgtttcg
     1081 agcatttcgt cggcgacacg caaggccgcg acaatgtcac gacggcggaa atggcgctcg
     1141 acgagaaggg caagttcctc gccttgcgcg tcgacattct cggcaatctc ggcgcttatc
     1201 tgtcgcaatt cggaccgtac attccctatc tcggcgcgtc gatggcgacc ggcgcctatg
     1261 acattcccgt tttgcacgcg cgggtgcgcg gaatctacac gcatacgacg ccggtcgacg
     1321 cctatcgcgg ggccggccgg ccggaagcgg cctatgtgct cgagcggctg gtcgatcttt
     1381 gcgcgcggaa attgtcgatg ccgcgcgaag aaatccgcgc gctgaatttc gtcaagccgt
     1441 cgcagatgcc gtaccacacg cagacggacc gcacctacga tgtcggcgat ttcgagggcg
     1501 cgatgcgcgc ctcgctggcc aaggccgatt acgccggctt cgacaaacgc gccgaggcgg
     1561 cgaaagcgcg cggcaagctg cgcggcatcg gctttgcgag ctatatcgaa tgcaccgcct
     1621 ggaacgacaa cgaggaaggt tcggtcgagc tcggtccgga cggcaatttc acggtgctga
     1681 tcggcacgca atccaacggc cagggccatg agacggctta cgcgcaggtc gtctcgcaat
     1741 atctcgatgt gccgctcgag cgcatcaagg tcgttcaggg cgacacggac cgcgttaaaa
     1801 ccggcttcgg caccggcggc tcgcgctcga ttccgatcgg cgcggtgatg gtcggcaaag
     1861 cgtcggaaaa gctcgtcgaa tcgttgaagg agatcgccgc ggacaagctc gaagccgcgc
     1921 cggcggattt ggaggtcgcc gacggccgca tccgcatcgc cggcaccgac cgctcgatct
     1981 cctacgaaga gatcgccaag ctgcccggcg cgacgccgga aaagctcaag gcgggcggcg
     2041 aatatgcgcc ggccgacgcg acctatccga acggcacgca tacatgcgaa gtcgagatcg
     2101 accccgagac cggcgtcgtc acgatcgagc gctacacggt ggtcgacgat ttcggcttca
     2161 cgctcaatcc gctgctgctc gccggccagg tccatggcgg catcgcgcaa ggcatcggcc
     2221 aggcgctgca tgagcgcgcc gtgttcagcg aggaaggaca attgctgacc gcgagcttca
     2281 tggactattg catgccgcgc gccgacgact tgccgaactt ccatttcgag acgcgcaaca
     2341 ttccctcgac caccaatccg ctcggcctca agggcgcggg cgaagccggt tcgatcggct
     2401 cttcgccggc ggtgatgaac gcggtcagcg acgcgctctg gcggcgctac ggcgagcatc
     2461 ggatcgacat gccggcgacg ccttttgcga ttttccgcgc gatccaaggg gcggcgaaca
     2521 cttgacggcc ggcaggtatt cgcaaaacgg gacttcacga aaaagagtac ggcccgccgc
     2581 cacgtcgcgc ttgcctccct ttgaagcttt cgccaacttc ggcttgaaag ggaggcggac
     2641 aatgaagcga acgttgatca tcgttgcgac cctggcgctc ggcgtcggcg ccgtctatgc
     2701 ggcttccgat gcgattgcca agcgccaggc tttgatgaag gccaatggca aggcggcgac
     2761 cgcgatcgtc aagatcatga agggcgcgcc gttcgatctc gccgtcgtgc aggcttcgct
     2821 gaagaccttc atcgatgcgg ccgagaaagg cccggctttg ttccccgacg acagcaagac
     2881 cggcgacaat accgaggcgc tgccggcgat ctgggagaac aaaaaggatt tcgaggcgca
     2941 tttcgccaag cttggcgacg acgcaaaggc ggcgctcggc gcgatcaagg acgaggcgag
     3001 cctcaaagcc gagatgccga aagtcttcga cgattgcggc ggctgtcacg agaaataccg
     3061 cgccaagaag cagtgagcgg ccggactttt cgcttgaatc ccgaaaggct ctggaacgaa
     3121 agcggatggc ggcttgcgcc tcccgctctc gccgaatgcg cagtcattta accggcgctc
     3181 cgggcgccgc aatctcgccg gcgcggcctt tgccgcgctg ccgcgccggg gccggacgat
     3241 gagacgttgg ctgcttcttc tgctgcttgt cgctgtcgcc gccgcgatcg cggcgtggtt
     3301 cgtctcggcg ccgcgccctc tctatgcgcg cgccgattgg cgcgcctacg aggatggcgg
     3361 cgatcccggt cgcggccgga tcatattcct ggccggcgga tgcgaatcct gccacatgac
     3421 gcctggagag aacgatccat tgaagcttgg cggcggctac gaattgaaga cgccgttcgg
     3481 ttcgttcttc ccgcccaaca tctcgcctga tccgcaggac ggaatcggcg gatggaaagt
     3541 cgtcgacctc gccaacgcgc tgctcgtcgg cgtctcgcct gaacctgcgc atctttatcc
     3601 cgcattgccc tacacgtcct acagccatat gaaattcgag gacgtgcgcg acctgatggc
     3661 ctatctgcgc acgctgccgc cggttaaggg ccgcgcgccg cctcatcgtt tgtcctttcc
     3721 cttcaacatc cgccgcggcg tcggcttttg gaaattgttg tacctcaaag gcggcgttcc
     3781 ggccgacgat ccggcccaga gcgcggcgtg gaatcgcggc cgctatctcg tcgaagggcc
     3841 cggccattgc gccgaatgcc actcgccgcg cgacatattc ggcggcatcg tcgccgcgaa
     3901 gcgcttctcc ggcggcctga cggcggacgg caagcacaag gtcccgaaca tcacccaagg
     3961 cggcgcgctg gcgaaatggt cgcaatcgga catcgaaacc gcgctgtcgc tcggcctgac
     4021 gccggacggc gattcgctcg gcggcgacat ggcgccggtg gtgcgcaatc tcagccaatt
     4081 gccgaaagac gacatcgccg cgatcgcggc ttacatcaag agcctgccgg cgatcccgtc
     4141 ggccggcaaa tagccgcgct ttacgccgat tggacggccg cggcggcgtc ggcgcggatg
     4201 cgctcgatca tcgcccgcac gccgttcgag cgctgcggcg tcagatgctg ttcgagcccg
     4261 agctcgcgga acagagcgag cgcgtcggtg gcgagaattt tccgcgccgt gcggccggaa
     4321 aagagcgcga tggcgatggc gaccaggccg cgcacgagat gggaatcgct gtcgccgcga
     4381 aagatgagga ccggctcgcc gtccggcccc gccgccctgg tcatttccag ccagacctgg
     4441 ctgacgcagc cgcgcacctt gttggcgtcg ttatgcgcgg cctcgctcag cggctccaag
     4501 gtccggccaa gatcaatgac atagcgatac cggtcctccc aatcgtcgag gatggagaaa
     4561 tcgctgcgaa tttcgtcgat accggccaag aaagcctcgt cgtggcggcc gcgtgagccg
     4621 ccggagggca aactaagccc tctcgtccta gatagcgagc caggtcaacg cctcaagggg
     4681 cgaaaaggtc aggccccgga tttcaatagg cgtccgcgcc agcgcggggc gcgatcggac
     4741 gccgtcaggg aattggcgga cgggcggagg cccttcgcgg cggccttggc gtccttgccg
     4801 gctttcggcg cagaccgctc ggccggcgcc gtcgccaatc ggtcggcggc cagtagaacc
     4861 gcgccgcagg cgacagccga gccggcgcag ccggctttcg ccgccgcctc ggcatgatcg
     4921 gcgaggctgg ttttggcgtc gccgaggacg cgcgccattt cggtccgctc cgacggcatc
     4981 caggagaaga ccaggcagag ccagaacgcc gatcgcaaca ggaacatcat gggtcaaacg
     5041 ccgcaaacgg gctgctccgg ctttcgtctc cggagcgcgt agaggatagc cgcgggttgc
     5101 aaaaaccccg tcgcgttttt gcggcgcatt cggccgccgc gattaaccga tccgtaagct
     5161 ttcccgctgg gcggaaacgc tttttcaacc atggcgccaa gcaagcgtcg agaagcggcg
     5221 ccttggcggc gtgtcggcct cgcctcgcgg ccttccttag cgttcattta agcctggctt
     5281 gggatcgtcg gcagcggccg ccgaagcggc cccggagcgg cggattgacc tcattcatcg
     5341 accttttgtc gcgacgtttg tcggccttgc cgcgcctcta cgctcagcgc gcgagcgggg
     5401 cttccttcat ttatgcgcgg ctcgccgcct cggtcgcggc gctgatcctc acgccgctct
     5461 atctcgccgc cttcggcgcc cccgacgcct gggcgatctg cgccctggtc tggctcattc
     5521 tgccgctcgc ggcgatcgtc catgtggcgc gaaccggcaa gaccgtcgag gcgcagagcg
     5581 tctcgctcgc gtcgctgctg atcctcgcag cgatcgtcgc gctcggcgca cgcggctcgc
     5641 atgacctcat cttcgcttgg cttttgctgg cgccgctgga agccgcggcg tcgggctcgg
     5701 cggcccttct cgtcgccgcc tgcttcttcg ttgcaacgct gctggcgggt ctcgtcggcg
     5761 ccgaatattg gggcctcgtc ccggccggcg gcgccttgcc cgggccggcc gcggccgccc
     5821 ttgccgcgcc cgctgctctc tacggcctcg ttctttcgtt cgccgcgctc gctttgcatc
     5881 gcaggcgcgc cgacgtcgaa gcggaccaca acgagaatta ccgcgtcctc gccgacgcga
     5941 tcggcgatct cgtcctgcgc cacgatcaga gcggcgcggt gatctcggcg agcgccgccg
     6001 ccgagccgct gtttcagacg ccgccgcaag acctcctcgg ccgcggcttt ttcgagcgcg
     6061 tccatgtcgc cgaccgtccg gcttacctgc aggcgctcga cgacgcccag cagcgcgacg
     6121 gcgcggtggc gacgacgttg cgcctgcgcg tcggcggcgg cgacgagccg gtcttcgcct
     6181 g
//
LOCUS       DATGJW010000473         5245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1540362, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000473 DATGJW010000000
VERSION     DATGJW010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5245
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540362"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VKU03_14240"
     CDS             <1..404
                     /locus_tag="VKU03_14240"
                     /inference="COORDINATES: protein motif:HMM:NF012705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="squalene/phytoene synthase family protein"
                     /protein_id="HLJ72476.1"
                     /translation="PTGEKLADHLGRALQTTNILRDLDEDAGLGRLYLPKDALAKAGI
                     TSRDPAAVLAHPALETAAQVLVVEARDRFEKARAVMASCPRGNVRAPRLMAAVYRMIL
                     DRLARRGFEPPRIKVSAPRLAVLFVLLREMI"
     gene            405..1661
                     /gene="hpnE"
                     /locus_tag="VKU03_14245"
     CDS             405..1661
                     /gene="hpnE"
                     /locus_tag="VKU03_14245"
                     /EC_number="1.17.8.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxysqualene dehydroxylase HpnE"
                     /protein_id="HLJ72477.1"
                     /translation="MKRPVVHIVGAGLAGLSAAVRLSENGAAIVLHEAARQAGGRCRS
                     FFDETINATIDNGNHLLLSGNRAALAYLDAIGARSELTGPGRCEIAFADMATNERWTI
                     RVNEGPLPWWLLVEGRRTPGVRMLDYLGALRLLWAPKGARLSEVIACSGRLYERLWRP
                     FFLAALNTEPKQASAALAAATLRETLARGGRACHPLVAAHGLSRAFVDPALRLLQKRG
                     AQIRFERRLRSIEFSDGRAASLEFEHDSIALGQGDVAILAAPAHIAAALVPDLTTPQK
                     TSAILNGHFNIAPPAGTPPIIGVVNGTVEWIFTYPDRISTTVSGADRFMDESREKLAA
                     DLWRDIAALTNLPDSLPSWRIIKEKRATFAATPEEAARRPPAATRWRNLLLAGDYVNT
                     GLPATIEGAIRSGETAAALARERIGA"
     gene            complement(1716..2861)
                     /gene="hpnH"
                     /locus_tag="VKU03_14250"
     CDS             complement(1716..2861)
                     /gene="hpnH"
                     /locus_tag="VKU03_14250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012335985.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosyl-hopene transferase HpnH"
                     /protein_id="HLJ72478.1"
                     /translation="MGIPFRQMAGVGAYVLRQKLKGVKRYPLVLMLEPLFRCNLACAG
                     CGKIDYPDKILNQRLSVDQCLEAIDECGAPVVVIAGGEPLLHRELDQVVDKALARGKY
                     VTVCTNALLLEKKLPAFRPHPRFNWSIHLDGDEEMHDRSVCQKGVYQRAVAALRAAKA
                     KGFRVNINCTLFDGADPSRVAAFLDETRRLGVEGVTISPGYAYERAPDQKHFLNRTRT
                     KQLFRDILAKGRGKKWAFYQSSLFLDFLAGNQSYHCTPWGNPTRTVFGWQRPCYLLGE
                     GYAKTFKELMETTDWDSYGVGAYEKCADCMVHSGFEASAVNDAFAHPLKLLKIAAKGI
                     NTGGAMAPEIRLDRQRPAEYVFSRHVEKKLAEIEADEARAPKRARAG"
     gene            3176..4480
                     /locus_tag="VKU03_14255"
     CDS             3176..4480
                     /locus_tag="VKU03_14255"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ72479.1"
                     /translation="MSSPSLTHRPRLAVLVDLPRSAEAGGHVKYWERIAGAAAEAPDF
                     PFDLTVYFSGGEADETPAAHVRLRHLRPVFSTERLKFLPYVPDHSDLASFHRRLARDL
                     PAYDLIHTTDAYFAFAKTAERIARRSGVPLTSSFHTDTPAYARIFTRQTIDRLLSRWP
                     WLRRKLIEDWRAPDRQGAKMERLLRRHLRAASHVFFTRAEDRRLAEEIRGAAGVSRLR
                     IGVDKNLFGPHRREQAALRRDYGIPEDRVIALFVGRVDIGKNVPVLIEAAQRALAADA
                     PLHLIVAGLGPMRTDLAERLAGHISLPGFVSPSELGRLYASADFVALPSEVEIGGMAV
                     AEAIAAGAPMLLARKASSASVFDHTEAIRLVEGGAEAWTAALAEMASSAESRRGMRQA
                     ALDYGARGLASWSDVLREDLTPGWRAALARASKEQAWRNGDA"
     gene            4473..>5245
                     /locus_tag="VKU03_14260"
     CDS             4473..>5245
                     /locus_tag="VKU03_14260"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HLJ72480.1"
                     /translation="MHERFGARILVLVPHPDDEVVAAAASLGRAKQQGAALFALYLTD
                     GCLSRETMWPWRRAHHGAAVARRRAEAERAAALLGLAPVGWGERPARFLWRDMAAVHA
                     EIRAAVAACKPDQIWVPAYEGGNPDHDALNALGQLLVGELSVFEFAEYNFFGGKARAQ
                     EFPYPNGKETIVELTAAEREKKRSLLRLYVSEQNNLGYVGIDRECFRPLAAYDYSRPP
                     HPGTLWYARFQWVPFRHPRVDFTPPEQVSKAICESLTSL"
BASE COUNT          839 a         1757 c         1707 g          942 t
ORIGIN      
        1 ggccgaccgg cgaaaagctc gccgatcatc tcggccgcgc cttgcagacg accaatatct
       61 tgcgcgatct cgacgaggac gccggactgg gtcggctcta tttgccgaaa gacgcgctcg
      121 ctaaagccgg cattacaagc cgcgatcccg ccgcggttct cgctcatccc gctctcgaaa
      181 ccgcggcgca agtcctggtc gtcgaggccc gagatcgttt tgaaaaggcg cgcgcggtca
      241 tggcctcctg tccgcgcggc aatgttcgcg cgccccgcct gatggccgcg gtctatcgca
      301 tgatcctcga tcgtcttgcc cgccgcggct tcgaaccgcc gcggatcaaa gtcagcgcgc
      361 cgcggctcgc cgtgctcttc gtcctgctcc gcgagatgat ttagatgaag cggccggtcg
      421 ttcatattgt cggcgccggc ctcgccggcc tttcggccgc ggtcaggctc tccgaaaatg
      481 gggcggcgat cgtcttgcat gaggccgcgc gccaagccgg cgggcgctgc cgctccttct
      541 tcgacgaaac catcaatgcg acgatcgaca acggcaacca ccttcttttg tccggcaatc
      601 gcgccgcgct cgcctatctc gacgcgatcg gcgcgcgcag cgagctgacc ggtccgggcc
      661 gctgcgaaat cgccttcgcc gacatggcga ccaacgagcg ttggaccatc cgggtgaacg
      721 aggggccttt gccctggtgg cttctcgtcg aggggcggcg cacgcccggc gttcgcatgc
      781 tcgattatct cggcgcgctg cgcctgctgt gggcgccgaa aggcgcccgg ctttcggagg
      841 tcatcgcctg ttcgggccgg ctctacgagc ggctgtggcg ccctttcttc ctcgccgccc
      901 tgaacaccga gccgaagcag gcttcggcgg cgctcgccgc cgccacgttg cgcgagactt
      961 tggcgcgcgg cggccgcgcc tgtcacccgc tcgtcgccgc ccacggcctg tcgcgcgcct
     1021 ttgtcgatcc ggctttgcgg ctgctacaga aacgcggcgc gcaaatccgc ttcgagcgcc
     1081 ggctgcgcag catagaattc tcggacggcc gagcggcgag cctggagttc gagcatgatt
     1141 cgattgcgct cggccaaggc gatgtcgcaa tcctggcggc gcccgcccat atcgccgccg
     1201 cgctcgtgcc cgacctgacg acgccgcaga agaccagcgc gatcctcaac ggccatttca
     1261 acatcgcgcc gccggccgga acgccgccga tcatcggcgt ggtcaatggt acggtcgagt
     1321 ggattttcac ctatccagac cggatctcga cgacggtcag cggggcggac cggttcatgg
     1381 acgaatcgcg cgaaaagctc gccgccgatc tctggcgcga tatcgccgcg ctgaccaacc
     1441 tccccgattc gctgccgtcc tggcgcatca tcaaagagaa gcgcgcaacc ttcgcagcaa
     1501 cgcccgaaga ggcggcgcga cgcccgccgg ccgcgacgcg ctggcgcaac ctcctgctcg
     1561 ccggcgatta tgtgaatacc ggcttgccgg cgacaatcga aggggcaatc cgctcgggcg
     1621 agacggcggc ggccttggcg cgggagcgga tcggggcttg agacgggcct tcagtgcttc
     1681 gcgtcgcccg caatgacagc taaagtcgct cggcgtcacc ccgctcgcgc ccgcttcggc
     1741 gctcgcgcct cgtccgcctc gatctcggcg agcttcttct cgacatggcg cgagaacacg
     1801 tactcggccg gccgctgccg gtcgagccgg atttccggcg ccatcgctcc tcccgtgttt
     1861 atccccttgg cggcgatctt cagcaacttc agcggatggg cgaaggcgtc gttcaccgcg
     1921 ctcgcctcga agccgctatg gaccatgcaa tcggcgcatt tttcgtaagc gccgacgccg
     1981 tacgaatccc agtcggtcgt ctccatcagc tccttgaacg tcttcgcata gccttcgccg
     2041 agcaggtagc aaggccgctg ccagccgaac accgtgcgcg tcggattgcc ccagggcgtg
     2101 caatgatagc tctgattgcc ggccaagaaa tcaaggaaca gcgaggattg gtagaaggcc
     2161 catttcttgc cgcggccttt ggcgagaatg tcgcgaaaca attgcttggt gcgcgtgcgg
     2221 ttgaggaaat gcttctgatc cggcgcgcgc tcataggcgt agccgggcga gatggtcacg
     2281 ccctcgacgc cgagccgccg agtctcgtcg agaaaggcgg cgacgcgcga aggatcggcg
     2341 ccgtcgaaca gggtgcaatt gatgttgacg cgaaagcctt tggctttggc ggctcgcagc
     2401 gccgccacgg cgcgctgata gacgcccttc tggcagaccg atcggtcatg catctcctcg
     2461 tcgccgtcga gatgaatcga ccagttgaag cgcggatgcg gcctgaaggc cgggagcttc
     2521 ttttcgagca gcagcgcgtt ggtgcagacg gtcacgtatt tgccgcgcgc cagagccttg
     2581 tcgacgacct ggtcgagctc gcgatgcagc agcggctcgc cgccggcgat gaccacgacc
     2641 ggcgcgccgc actcgtcgat cgcctccagg cactgatcga ctgaaagccg ctgattgagg
     2701 attttgtccg gataatcgat cttgccgcaa ccggcgcagg cgagattgca gcgaaacagc
     2761 ggctccagca tcaggacgag cgggtagcgc ttgacccctt taagtttctg gcgaagcacg
     2821 taggcgccga cgccggccat ttgtctgaag ggtatgccca agcggagagt cctttcgcga
     2881 agaagcgcgc ggcgatcaaa gaaagccatt tcaacgtctg tattttgctt ttccctacat
     2941 caaccggcgc ataaaggaaa ttgccgctcc ggcggggcgc gaattccgga tttgcagggg
     3001 tcatgatgcc gatgcgcaac ccggcgcttt cgccccgcac ggggcatggc gcgggcgcgc
     3061 gcctcctgct aagcaggctc cccatttcgc caaaggatgc gaccgtcctc gccgcgtccg
     3121 cttgccgtag cggaaggcgt cggcgcgcct ttcgggacga accgcctgat acgccttgag
     3181 ttcaccctcc ctcacccatc gcccgcgtct cgccgtcctg gtcgacctgc ctcgatcggc
     3241 cgaggcgggc ggccatgtca aatattggga gcgaatcgcc ggcgccgcgg ccgaagcgcc
     3301 agattttcct ttcgacctga cggtctattt ttccggcgga gaagcggacg agacgccggc
     3361 ggcgcatgtc cgcttgcgcc acctcagacc ggttttcagc accgaaaggc tgaaatttct
     3421 tccctatgtg ccggaccatt cggaccttgc ctcatttcat cggcggctgg cgcgcgatct
     3481 gccagcctat gatctgatcc atacgacaga cgcttatttc gccttcgcca aaacggccga
     3541 acggattgcg cgccgcagcg gcgttcccct gaccagctcc tttcacaccg acacgcctgc
     3601 ttatgcgaga attttcaccc gtcagacaat cgaccggctc ctcagccgct ggccctggct
     3661 gcgccgcaag ctgatcgagg attggcgcgc gcccgatcgg caaggcgcga aaatggagcg
     3721 gttgctgcgg cgccatttgc gggcggcgtc gcatgtcttt ttcacgcggg cggaggatcg
     3781 ccgccttgcc gaagagattc gcggcgccgc gggcgtcagc cggttgcgga tcggcgtcga
     3841 caagaatctc tttggcccgc atcgtcgcga gcaggccgcg ctgcgccgcg attacggcat
     3901 tcccgaggac cgcgtcatcg ccctgttcgt cggccgcgtc gatatcggca agaacgttcc
     3961 ggtgctgatc gaggcggcgc agcgcgccct cgcggccgac gcgccgctcc atctgatcgt
     4021 cgccgggctt ggcccgatgc gaaccgacct cgccgagcgc ttggccggcc atatcagcct
     4081 gccgggcttc gtctcgccca gcgagctcgg caggctctat gcgagcgccg atttcgtcgc
     4141 gcttccgtca gaagtggaga tcggcggcat ggccgtcgcg gaagcgatcg ccgccggcgc
     4201 gccgatgttg ctggcccgca aagcgagctc ggcgagcgtc ttcgaccata ccgaggcgat
     4261 ccgcctggtc gagggcggcg ccgaggcttg gaccgcggcg cttgcggaaa tggcctccag
     4321 cgccgaatcg cggcgcggca tgcggcaggc ggcgctcgat tacggcgcgc gcgggctggc
     4381 gagctggtcc gacgtgttgc gggaggatct gacgccgggc tggcgcgcgg cgctggcgcg
     4441 ggcaagcaaa gaacaggcgt ggcgaaacgg cgatgcatga gcggttcggc gcgcggattc
     4501 tcgttctcgt gcctcacccg gacgacgagg tcgtcgcggc ggctgcttcg ctcggccggg
     4561 cgaaacaaca aggcgcggcg ctgttcgccc tctatctgac cgatggctgt ctgtcgcgcg
     4621 agacgatgtg gccatggcgg cgcgcccatc atggcgccgc ggtggcgcgc cggcgcgccg
     4681 aggcggagcg cgcggcggcg ctgctcggcc ttgcgccggt cggctggggt gagcggccgg
     4741 cgcgctttct atggcgcgac atggccgcgg ttcacgcgga gattcgagcc gcggtcgccg
     4801 catgcaaacc ggaccagatc tgggtcccgg cttatgaagg cggcaatccg gatcacgacg
     4861 cgctgaatgc gctcggccaa ttgttggtcg gcgagctgag cgtcttcgaa ttcgcggagt
     4921 acaatttttt tggcggcaag gcgcgcgcgc aggaatttcc ctatccgaac gggaaggaga
     4981 cgattgtcga actgaccgcc gcggagcggg agaaaaagcg ctcgctgctg cgactctacg
     5041 tgtccgagca gaacaatctg ggttatgtcg gaatcgaccg cgaatgtttc cgtccgctgg
     5101 ccgcctatga ttatagccgg ccgccgcatc cagggacctt atggtacgcc cgcttccaat
     5161 gggtcccctt ccgccatccg cgcgtcgatt tcacgccgcc cgagcaggtg agcaaggcga
     5221 tttgcgagag cctgacttcc cttat
//
LOCUS       DATGJW010000474         5264 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1540428, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000474 DATGJW010000000
VERSION     DATGJW010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5264)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5264)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5264
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540428"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(241..325)
                     /locus_tag="VKU03_14265"
     tRNA            complement(241..325)
                     /locus_tag="VKU03_14265"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(289..291),aa:Leu,seq:caa)
     gene            479..1060
                     /locus_tag="VKU03_14270"
     CDS             479..1060
                     /locus_tag="VKU03_14270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029990.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YqaA family protein"
                     /protein_id="HLJ72481.1"
                     /translation="MFKRLYHWVLSLSESPHAPAALAAVAFAESSFFPLPPDIILVPM
                     SLAKPQRAWRYALICTIASVAGGVLGYAIGALLFDTIGQWLIHVYGYGQRIAELKDLY
                     ARWGALVILLKGLTPIPYKLVTIVSGLLGYNFAAFVLLSILTRGARFFILAGALNLFG
                     DPLRLALERHFALFMGVIAVTIVAGFWIAARVF"
     gene            1060..1581
                     /locus_tag="VKU03_14275"
     CDS             1060..1581
                     /locus_tag="VKU03_14275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174139.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="disulfide bond formation protein B"
                     /protein_id="HLJ72482.1"
                     /translation="MAGVGALPANPRFNIAIVILLIAAATIAGAWIFQAFGYEPCELC
                     LKQRYAYYGGVPIAALLAYASTGRARGIVAAGMIGLALIFAANAVLGAYHAGVEWRFW
                     PGPAGCTGVIAGPQNAADLLKTLQHAKVVACDEVQLRILGLSLAGWNALISAALAALA
                     VLGAMAKGGEAAK"
     gene            1705..2724
                     /locus_tag="VKU03_14280"
     CDS             1705..2724
                     /locus_tag="VKU03_14280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0104 family protein"
                     /protein_id="HLJ72483.1"
                     /translation="MGKTWAGPQRLERLVAALEGRQALLHRLGVAGSLIIVAVSLFFF
                     ARAIVGIDVRQFRAAIAGTGGDQIAGAFLLAGLSYLALTGYDALALRHLRAKTPYRLT
                     ALASFASYAISFTLGFPLITAGAVRYWIYGPAGLSAGKVASLTLIAGITFWLGMGLVV
                     GVGLVSEAPAISEINHFHIAINQIFGLGVLALLAAYLAFVSQRGVKPILFHVRLPGPL
                     LTLGQTALGVIDVCAAAGALYVLLPKGHGLAFVTFGALYSFACMLGIASHAPGGLGVF
                     EATMLKTVPAESGSVLASLLLFRAIYYLTPFILAMALLGAHEAGRRWRSLREAIEKSS
                     GADDE"
     gene            2787..3884
                     /locus_tag="VKU03_14285"
     CDS             2787..3884
                     /locus_tag="VKU03_14285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ72484.1"
                     /translation="MSDISLSDLQADVRSQFCADLIGAMPEPAVMVGNDLRLLAANAS
                     ARNLLPAAKMGEPLVLALRAPDVLDGLRRVLAQGVAETVSWSERVPVERLFDVHIAPL
                     IARGGAVGAALITLGDLTEARKVERMRVDFIANASHELRTPLASLLGFIETLQGPARE
                     DEKARQKFLAIMREQARRMARLIDDLLSLSRIEQNQHVRPETPVDLALIARHVADTLA
                     PLAREMAIDLNVAADRPVIVTGDRDELVRVAENLVENAIKYGAPPPGGERRGKIEIRV
                     ERLDHEGSLSVRDDGPGIAPEHLPRLTERFYRIDAGQSRSKGGTGLGLAIVKHVIARH
                     RGRLAIVSEIGRGSAFTAIIPLHPGDSAPKS"
     gene            4018..5049
                     /gene="pstS"
                     /locus_tag="VKU03_14290"
     CDS             4018..5049
                     /gene="pstS"
                     /locus_tag="VKU03_14290"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008143474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0042301 - phosphate ion binding [Evidence
                     IEA];
                     GO_process: GO:0035435 - phosphate ion transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter substrate-binding
                     protein PstS"
                     /protein_id="HLJ72485.1"
                     /translation="MFLRPLTSAAILLAAIAGANAADISGAGATFPYPVYAKWADAYK
                     KETGDNVNYQSIGSGGGIKQIKAKTVTFGASDMPLKPKDVEASGLVQFPTVVGGDVPV
                     VNLDGVKPGDITLDGPTLAKIFLGEITKWDDPAIKALNPNAKLPSDAILVVHRSDGSG
                     TTFIWTDYLSKVSADWKAKVGASTAVEWPTGIGAKGNEGVANNVANTKGAIGYVEYAY
                     VKQNKMTYVDMVNKNGKQTTPDAASFQAAAANADWAKSDHYYVILTDQSGDKSWPIAG
                     ATFILMHKQPSDPAAAAEALKFFNWAYTKGGEMAQSLDYVPLPPAVVAQIRATWASEI
                     KGADGKPLM"
BASE COUNT          899 a         1768 c         1633 g          964 t
ORIGIN      
        1 aacgacaacg actcgacatt cgaaatcgac caatcttgca acagactgac cgaatttaca
       61 aacttggttt ggccacaatg cggccgcgcg agcgcgcctc cgacggcgac gccggaacga
      121 aataaacgaa cggcgtaacg cggccagacc gcagggcatt ctgtaaaagc gcggccgcct
      181 atgcttcggc gggcaagaat cgggagagcg cgcatagcgc aggcgccgca accggcgcct
      241 tggtgcccct ggccggaatc gaaccagcac tccttgcgga actcgatttt gagtcgagcg
      301 cgtctaccag ttccgccaca ggggcgagcc aaaagctcgg ggccgcccta tatcgcgagc
      361 gggcggcgag ggtcaacacg cctccgcggg ccgctcgcgc ccggccgcta gccgcgatgg
      421 acagcgggcc gccgctcctc tatcagcgtc gaacctgccg gggcccttaa aggattcaat
      481 gttcaagcgc ctttaccact gggtcttgtc gctctccgaa agtccgcatg cgccggccgc
      541 cctcgccgcc gtcgccttcg ccgaaagctc ctttttcccg cttccgccgg acatcattct
      601 ggtgccgatg tcgctcgcca agccgcagcg cgcctggcgc tacgcgctga tctgcaccat
      661 cgcctcggtc gctggcggcg ttctcggcta tgcgatcggc gctttgctct tcgacacgat
      721 cggccagtgg ctgatccatg tctatggcta tggccagcgg atcgccgagt tgaaggacct
      781 ctacgcgcgc tggggcgcgc tggtcatcct gctcaaaggc ctgacgccga ttccctacaa
      841 gctggtgacc atcgtttcag gattgctcgg ctataatttc gcggctttcg tcctgctttc
      901 gatcctcacc cgcggcgcgc gctttttcat cctggccggc gcgctcaacc tgttcggcga
      961 cccgctgcgg ctggccttgg agcgccattt cgctctattc atgggcgtca ttgcggtcac
     1021 catcgtggcc ggattctgga tcgcagcgcg agtgttttaa tggccggcgt cggcgcgctt
     1081 cccgccaatc cgcgattcaa catcgcgatc gtcatccttc tcatcgccgc cgcgacgatc
     1141 gccggcgcgt ggatatttca ggccttcggc tatgagccat gcgagctctg cctcaagcag
     1201 cgctacgcct attatggcgg cgtgccgatc gccgcgctgc tcgcctacgc ctcgaccggc
     1261 cgggcgcgtg ggatcgtcgc cgccgggatg atcggcctcg ccttgatttt cgccgccaac
     1321 gccgtgctcg gcgcctatca cgcgggcgtt gaatggcgct tctggccggg cccggctggc
     1381 tgcaccggcg tcatcgccgg gccgcagaac gccgccgatc ttttgaagac attgcagcac
     1441 gccaaagtcg tcgcctgcga cgaagtccag ttgcgcattc tcgggttgtc gctcgccggc
     1501 tggaacgcgc tgatctcggc ggcgctggcg gcgctcgccg tccttggcgc gatggccaaa
     1561 gggggcgagg cggcgaaata aagccgaggc ggcgcggcgt cgcccggctt gcccgtccgg
     1621 tcctgtcccg ccatatacgc gccgggccgg gaaaccagca tcaggaggcg catggcgaga
     1681 acaacagaaa aacgcgcgct tcggatgggg aagacctggg ccggcccgca acggctcgaa
     1741 cgcctcgtcg ccgccctcga aggccggcag gcgctgctcc atcggctcgg cgtcgccggc
     1801 agcctgatca ttgtcgccgt ctcgctcttc ttcttcgcgc gcgccattgt cgggatcgac
     1861 gtcaggcaat tcagggcggc gatcgccggc accggcggcg accagatcgc cggcgctttc
     1921 ctcttggccg gcttgagcta tctcgccttg accggctacg acgcgctggc gttgcgccat
     1981 ttgcgcgcca agacgcctta tcggctgacc gcgctcgcct ccttcgccag ttatgcgatc
     2041 tccttcaccc tgggattccc gctgatcacg gccggcgccg tccgctattg gatttatgga
     2101 ccggccggcc tttcagccgg aaaagtggcg agccttaccc tcatcgccgg catcaccttc
     2161 tggctcggca tgggcttggt ggtcggcgtc ggcctcgtca gcgaggcgcc ggcgatcagc
     2221 gagatcaatc atttccacat cgccatcaac cagatcttcg gtctcggcgt tctggcgctg
     2281 ctcgccgcct atctcgcctt tgtcagccag cgcggcgtca agccgattct tttccatgtc
     2341 aggctgcctg ggccgctgct gacgctgggg caaacggcgc tcggagtcat cgacgtttgc
     2401 gccgcagccg gcgcgctcta tgtgttgctg ccgaagggcc atggcctcgc tttcgtgacc
     2461 ttcggggcgc tctattcctt cgcctgcatg ctcggcatcg ccagccatgc gccgggcggc
     2521 ctcggcgtct tcgaagcgac aatgctgaag accgtgcctg ccgaaagcgg ctcggttctc
     2581 gcctcgctgc tgctgttccg ggcgatctat tatttgacgc cgttcatcct cgccatggct
     2641 ttgctcggcg cgcacgaagc gggccggcgc tggcgctcgc tgcgcgaggc gatcgaaaag
     2701 tcgagcggag cggacgacga gtgaaggcgg cttcccttgc ccgcgcgccg tccgcctcca
     2761 atcggccctg aggggacgcg ccggccgtga gcgacatcag cctctccgat cttcaggcgg
     2821 atgtgagatc gcaattttgc gccgatctca tcggcgccat gcctgaaccc gcggtgatgg
     2881 tcggcaacga tctgcgcctc ctcgccgcca acgcgtcggc gcggaatttg ctgcccgccg
     2941 cgaaaatggg cgagccgctc gtcctcgcct tgcgcgcgcc ggacgtgctt gacggcttgc
     3001 gccgggtcct cgcccagggc gtcgccgaga cggtctcgtg gagcgagcgg gtcccggtcg
     3061 agcgcctctt cgatgtgcat atcgcgccat tgatcgcgcg cggcggcgcg gtcggcgccg
     3121 ccttgatcac gctcggcgat ctgaccgaag cgcgcaaagt cgagcgcatg cgcgtcgatt
     3181 tcatcgccaa cgccagccat gagctgcgca cgccgctcgc ctcgctgctc ggcttcatcg
     3241 agacgctgca agggccggcg cgcgaggacg agaaggcgcg gcaaaaattc ctcgccatca
     3301 tgcgcgagca ggcgcggcgc atggcgcggc tgatcgacga tctgctgtcg ctgtcgcgca
     3361 tcgagcagaa ccagcatgtg cggccggaaa cgccggtgga tctcgcgctc atcgcgcggc
     3421 atgtcgctga tacgttggcg ccgctcgccc gcgaaatggc gatcgatctc aacgtcgccg
     3481 cggatcgtcc cgtgatcgtg acgggagatc gcgacgaact cgtccgcgtc gccgagaatc
     3541 tcgtcgagaa cgcgatcaaa tatggcgcgc cgccgcccgg cggcgaacgg cgcggcaaaa
     3601 tcgaaatccg cgtcgaacgg ctcgatcacg aaggctcgct tagcgtcagg gacgacgggc
     3661 ccggcattgc gcccgagcac ttgccgcggc tgaccgagcg cttctatcgc atcgatgccg
     3721 gccagagccg ctcgaagggc ggcaccgggc tgggcctcgc catcgtcaag cacgtcatcg
     3781 cgcgccaccg cggccgcctc gccattgtga gcgagatcgg ccgaggctcg gcctttaccg
     3841 cgatcattcc gctgcacccc ggcgattccg cgcccaaaag ctgatatttc agaagcttac
     3901 attgtcatca aacagtagtg gacctgtcat agataaaaga ccttggctcc gtagaaagag
     3961 ggccgaccgc cggccttcgg gctggcgcgt tccgcaaacc gagccagaga ggcaatcatg
     4021 tttcttcgtc cgctgaccag cgcagcgatt ctgctcgccg ccattgccgg cgcaaacgcc
     4081 gccgacattt caggcgccgg cgcaactttc ccctatccgg tctacgccaa atgggccgac
     4141 gcgtacaaga aagagacagg cgacaacgtc aactatcagt cgatcggatc cggcggcggc
     4201 atcaagcaga tcaaggccaa gactgtgacc ttcggcgctt cggacatgcc tcttaagccc
     4261 aaggacgtgg aggcgtccgg cttggtgcag tttccgaccg tggtcggcgg cgacgtcccg
     4321 gtcgtaaatc tcgacggcgt caagcccggc gacatcacgc ttgacgggcc gacgctcgcg
     4381 aagatttttc ttggcgagat tacgaaatgg gacgatccgg cgatcaaggc tttaaatccg
     4441 aacgccaaat tgccgagcga cgccattctc gtcgtgcatc gttcggacgg atccggcacg
     4501 acgttcatct ggaccgacta tctctcgaaa gtgagcgcgg attggaaagc gaaagtcggc
     4561 gccagcaccg cagtcgagtg gccgaccggc atcggcgcca aagggaatga aggcgtcgcc
     4621 aacaacgtcg ccaacaccaa aggcgcgatc ggctatgtcg aatacgccta tgtcaagcag
     4681 aacaagatga cctatgtcga tatggtcaac aaaaacggaa agcaaacgac gcccgacgcg
     4741 gcgagctttc aagcagccgc ggcgaatgcg gattgggcga aatctgatca ctactacgtc
     4801 attttgaccg accaatccgg cgacaaatcg tggccgatcg ccggggcgac cttcattctc
     4861 atgcacaaac agccttctga tccggcggcg gcggccgaag ctctcaaatt cttcaattgg
     4921 gcttacacga aaggcggcga aatggcgcaa tccctcgact atgttccgct cccgcccgct
     4981 gtggtcgcgc agatcagggc gacgtgggcg agcgaaatca aaggcgcgga cggcaagcct
     5041 ctgatgtgaa cggacaaagg ccttgaacgc gcggacggcg ttggcccggc cgcgcgctcg
     5101 cgccgcgacg accaaccagg cgaagcgatt gagaatgtcc ggtggctgat atttcgatcc
     5161 cgatcgcgcc gattcttgca tcgaacaatg ccggctcgcg cgctcgcgcg ctgcgccgcc
     5221 tgattcttcg cgatcatgcg ttccgcttca tcacccgcgc ggcg
//
LOCUS       DATGJW010000475         3088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_301113, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000475 DATGJW010000000
VERSION     DATGJW010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3088
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301113"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..504
                     /gene="prs"
                     /locus_tag="VKU03_14295"
     CDS             <1..504
                     /gene="prs"
                     /locus_tag="VKU03_14295"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HLJ72486.1"
                     /translation="SAPVMVRDIKSRATFNDLMVVSPDVGGVVRARALAKRLDAPLAI
                     VDKRRERAGESEVMNIIGDVRGRNCLLIDDIVDSGGTLCNAADALLEKGALSVSAYIT
                     HGVLSGGAVARIAASKLRELVLTDTIQPTEAVRVAKNIRVISIAPLIGEAIARIAKEE
                     SVSVLFD"
     gene            849..1865
                     /locus_tag="VKU03_14300"
     CDS             849..1865
                     /locus_tag="VKU03_14300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009494558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HLJ72487.1"
                     /translation="MVNCKSGVSAAGVGATFAFALLASTALAADIKPAIIYDLGGKFD
                     KSFNEGVYHGAEKFKKDTGIAYRDLEIQNDAQREQALRKFVKDGFNPILTVGFAWATA
                     LDKIAAESPKTEFGIIDMVVNKPNVRSIVFKEQEGSFLVGVIAAKTTKTDKVGFVGGM
                     DIPLISKFECGYKQGVKYVNGKDEIFANMTGTTPAAWNDPVKGGELAKSQMDRGADIV
                     YAAAGATGQGVLKAAADAGKFGIGVDSDQNYLFPGHVLTSMMKRVDIATDRFFTDAKN
                     GTLKGGVEALGLKEGGVGYALDQYNEKILSPEAKAAADAAKADIISGKIQVHDYMSDN
                     KCPV"
     gene            2007..>3088
                     /locus_tag="VKU03_14305"
     CDS             2007..>3088
                     /locus_tag="VKU03_14305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758275.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72488.1"
                     /translation="MKVDAGKRPPAIELVGIDKRFGRVHANNNVNLKVERGVIHGIVG
                     ENGAGKSTLMSILYGFYEADAGEIRVNGEPVRIRSSREAISHGIGMVHQHFMLVQPLT
                     VLENVMLGAEGGPLLAAGAGQIRAKLAELAREYGLVVDPDAVVGELSVGLQQRVEILK
                     ALVRGAEILVLDEPTAVLTPAEADQLFEMLRVLRSQDKTVIFITHKLREIMRLTDRVS
                     VMRLGEMVANFETAKTSPPELADAMVGRKVILRIEKGQARPGEVALEARNLIVKDDRG
                     VVRIDNMSFVLRRGEIVGIAGVAGNGQSELLEALAGIRPLAGGEILIAGQAPPPEMRN
                     PKGMRKLGVFHVPEDRHRMGLVMPFQA"
BASE COUNT          586 a          992 c          994 g          516 t
ORIGIN      
        1 tcggcgccgg tcatggtgcg cgacatcaag tcgcgcgcga cgttcaacga tttgatggtc
       61 gtttcgcccg atgtcggcgg cgtggtgcgc gcccgggcgc tcgccaaaag gctcgacgcg
      121 ccgctggcga tcgtcgacaa gcgccgcgag cgggcgggcg aatccgaggt gatgaacatt
      181 atcggcgacg tgcgcggccg caattgcctg ctgatcgacg acatcgtcga ttccggcggc
      241 acgctgtgca acgccgccga cgcgctgctc gagaaaggcg cgctcagcgt ctcggcctac
      301 atcacccacg gcgtgctctc cggcggcgcg gtcgcgcgca tcgccgcctc gaagctcaga
      361 gagcttgtgc tgaccgacac gatccagcca accgaggcgg tgcgcgtcgc caagaatatc
      421 cgcgtcatct cgatcgcgcc gttgatcggc gaagcgatcg cccgcatcgc gaaggaggag
      481 agcgtctcgg tcctgttcga ctgaggcggg gcgccgagga cccctcccca gaaagcgggg
      541 agaggtacgg agaggggccg gtcgtctggt tcgagcctaa ccgacctgcc cctctcctta
      601 cctctcccgt tgagcatcgc cctacgggga gaggggtcgc ggacgctggg ctctacacac
      661 gatcgctcgc gcgatcattg accggcccga cttaaagccg atgcccgttc gccgaatccg
      721 cttgacttcg tttttgcgcc acgcaatcgt tttgaccaat tggtcagaaa gggagtgggt
      781 cagccgcatc gcccggcggc ggatcgtctg atcacgcgtg ggcgtttcgt ttggcgaggg
      841 gagacaacat ggtcaattgc aaatccgggg tttcggcggc gggcgtcggc gcgacattcg
      901 ccttcgcgct tctggcgtcg accgcgttgg cggccgatat caagcccgcg atcatctacg
      961 atctcggcgg caagttcgat aagtccttca acgagggcgt gtaccacggc gccgagaaat
     1021 tcaagaagga caccggcatc gcctatcgcg acctcgaaat ccagaacgac gcgcagcgcg
     1081 agcaggcctt gcgcaaattc gtcaaggacg gcttcaaccc catcctgact gtcggcttcg
     1141 cctgggcgac ggcgctcgac aagatcgcgg ccgaatcgcc gaagaccgag ttcggcatta
     1201 tcgacatggt ggtcaacaag ccgaacgtcc gctcgatcgt cttcaaggag caggaaggct
     1261 cgttcctggt cggcgtcatc gcagccaaga cgaccaagac cgacaaggtc ggcttcgtcg
     1321 gcggcatgga cattccgctg atctcgaaat tcgaatgcgg ctataagcag ggcgtcaaat
     1381 acgtcaatgg caaggacgaa atcttcgcca acatgaccgg caccacgccg gcggcctgga
     1441 acgatccggt caagggcggc gagctcgcca agtcgcagat ggatcgcggc gccgacatcg
     1501 tctacgccgc cgccggggcg accggccagg gcgtgctgaa agcggcggcg gacgccggca
     1561 agttcggcat cggcgtcgat tccgatcaaa attatctatt tcccggacat gttctgacct
     1621 cgatgatgaa gcgcgtcgat atcgcgaccg accggttctt caccgacgcc aagaatggta
     1681 cgctcaaagg cggcgtcgag gcgctcgggc tgaaagaggg cggcgtcggc tacgcgctcg
     1741 accagtacaa cgagaagatt ctctcgccgg aggcgaaagc ggccgccgac gccgccaaag
     1801 ccgacatcat ctcgggcaaa atccaagtgc acgactatat gtcggacaac aagtgcccgg
     1861 tctgatctga agggaattgc cgcgatgcgt cccgcgaacg atttgccccg gtcgcgggac
     1921 gctgacgatc gaagccggtc attgcttcgc ttggctcgca atgacggctg agggtctgcc
     1981 gtgaagagcc gagcgggggc cgcctcttga aagtcgacgc cggcaaacgg ccgcccgcga
     2041 tcgagcttgt cggcatcgac aagcgcttcg gccgtgtgca cgccaacaac aacgtcaacc
     2101 tgaaagtcga gcgcggcgtc attcacggca ttgtcggcga gaacggcgcc ggcaagtcga
     2161 cgctgatgtc gatcctctac ggcttctacg aggccgatgc cggcgaaatc cgcgtcaacg
     2221 gcgagccggt ccgcatccgc tcctcgcgcg aggcgatcag ccacggcatc ggcatggtgc
     2281 atcagcattt catgctggtg cagccgctga ccgtgctcga aaacgtaatg ctcggcgccg
     2341 aaggcgggcc gttgctcgcc gccggcgccg gtcagatcag agccaagctc gccgaactcg
     2401 ccagggaata cggcctcgtc gtcgatccgg acgcggtggt cggcgagctc tcggtcggct
     2461 tgcagcagcg cgtcgaaatc ctgaaggccc tggtccgcgg cgccgagatt ctcgtcctcg
     2521 acgagccgac tgcggtgctg acgccggccg aggccgatca gctcttcgaa atgctgagag
     2581 tgctgaggtc gcaagacaag acggtcatct tcatcactca caagctgcgc gagatcatgc
     2641 ggctgaccga ccgcgtctcg gtgatgcggc tcggcgagat ggtcgccaat ttcgagaccg
     2701 cgaaaacttc gccgcccgag ctcgccgacg cgatggtcgg ccgcaaagtc attctgcgca
     2761 tcgaaaaggg ccaggcccgt cccggcgagg tcgcgctcga agcgcgcaat ctgatcgtca
     2821 aggacgatcg cggagtcgtc cgcatcgaca atatgtcctt cgtcctgcgc cgcggcgaga
     2881 tcgtcggcat agccggcgtc gccggcaacg gccaatcgga attgctcgaa gccctggccg
     2941 gcatccgccc cttggccggc ggcgaaatcc tcatcgccgg ccaggcgccg ccgcccgaaa
     3001 tgcgcaatcc gaagggcatg cgcaagctcg gcgttttcca tgtgccggaa gaccgccacc
     3061 gcatgggcct ggtcatgccg ttccaggc
//
LOCUS       DATGJW010000476         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1540663, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000476 DATGJW010000000
VERSION     DATGJW010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540663"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..476
                     /locus_tag="VKU03_14310"
     CDS             <1..476
                     /locus_tag="VKU03_14310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007758225.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HLJ72489.1"
                     /translation="LGEVAEILDKDETEVTALLDAAAREITEHVATDVLIIEDEPIIA
                     LDLEAIVQDLGHRIVGVARTRKEAVNMIGEHRPSLVLADIQLADGSSGLDAVNDILLS
                     IDAAVIFITAYPERFLTGDRPEPAFLLTKPFEPEAVKAAISQALFFDRKAGRKAA"
     gene            complement(514..681)
                     /locus_tag="VKU03_14315"
     CDS             complement(514..681)
                     /locus_tag="VKU03_14315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1328 family protein"
                     /protein_id="HLJ72490.1"
                     /translation="MFGWAIAFLIIAIIAAILGFGGIAGTAMAAAKIVFIVALIAFAI
                     SGVLGLTRRSY"
     gene            905..2416
                     /gene="glgA"
                     /locus_tag="VKU03_14320"
     CDS             905..2416
                     /gene="glgA"
                     /locus_tag="VKU03_14320"
                     /EC_number="2.4.1.21"
                     /inference="COORDINATES: protein motif:HMM:NF001899.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004373 - glycogen (starch) synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycogen synthase GlgA"
                     /protein_id="HLJ72491.1"
                     /translation="MKILIVTSEFADFAKTGGLADVSAALPRALRDKGIDVRVLLPGY
                     QKVVDGLQNLRIVATLPPYAGLPRCLIGEGRTSDDLAIYVVIEPALFQRNGGLYGAAN
                     GGGDWPDNDVRYARLSLAGAEIGEGKAALGWKPDLVHAHDWQTGLTPAYLRWRGAGAS
                     SVFTIHNLAHQGLYPAERCAALGAPESAFSINGVEFHGRLSFMKAGVYYADHVTTVSP
                     NYAREITEEAHGGGLHGLTAGLAAQGRLTGIVNGVGPEWDPSNDPDIGQPFDKEDTSA
                     KAWIKDRVRTGLCLAHRDGPLFGVVSRLVHQKGLDLVAEVAGDIVERGGQIAILGVGD
                     PVTEEMLRGVVRAHPDDIAMLNGYNEPMARRIMAASDFYLMPSRFEPCGLSQMHAQLY
                     GALPIVHATGGLVDTVEDGETGFVFRPFGKQSFLEAIGRAFSTYGAPGEIERMRRAAM
                     SRRYGYDDIVGEYIKIYAHLTGKPLLRVANNSETPRSGGVSATLRVARAMSLL"
BASE COUNT          466 a          863 c          843 g          492 t
ORIGIN      
        1 cgcttggtga agtcgcggag attttggaca aggacgagac cgaagtgacc gccctgcttg
       61 acgccgccgc tcgggaaatc accgagcatg tcgcaaccga tgttctgatc atcgaggacg
      121 agccgatcat cgccctcgat ctcgaggcga tcgtccagga cctcgggcat cggatcgtcg
      181 gcgtcgcccg cacccgcaag gaggcggtca acatgatcgg cgagcaccgg ccgagcctgg
      241 tcctcgccga tattcagctc gccgacggca gttccgggct cgacgcggtc aacgacattc
      301 tgttgtctat cgacgcggcg gtgatcttca tcaccgccta tcccgagcgc ttcctcactg
      361 gcgaccggcc tgagccggcc ttccttttga ccaagccgtt cgagccggaa gcggtgaagg
      421 cggcgatcag ccaagcgctc tttttcgatc gcaaggccgg ccgcaaggca gcctgacctc
      481 cttttagcga tttggcgcgc cgcgcggcgc gccttagtag gatcggcggg ttaagccgag
      541 gacgcccgaa atcgcgaagg cgatcagcgc gacgatgaaa acgatcttag cggcggccat
      601 ggcggtgccg gcaatgccgc cgaagccgag aatggccgcg atgatcgcga taatcaggaa
      661 agcgatagcc caaccgaaca tcggtctctc ctccagctgc ggcggcgctg cgccgaacaa
      721 cgaagtccgg cggatataac gcgccctcgc cgggaggggt tcctgaatgg ggagccggtc
      781 tgtcgaagga cccggccggg aacacggcgc gggcgtccta agttgataat gaggggcttg
      841 cggcgtcgag cttcacaacg tcctcttgta tcaggcgtcc ctcgcaatcg gccgcgtcgc
      901 gttgatgaaa atccttattg tgaccagcga gttcgctgat ttcgccaaga ccggcggcct
      961 cgccgacgtt tcggccgcct tgccgcgcgc cttgcgcgac aaaggaatag acgtccgggt
     1021 gctgctgccc ggctaccaaa aggtcgtcga cgggcttcag aacttgcgca tcgtcgcgac
     1081 cttgccgccc tatgccggct tgccgcgctg cctgatcggg gaagggcgaa ccagcgacga
     1141 tctggcgatc tatgtcgtca tcgagccggc gctttttcag cgcaacggcg gcctctacgg
     1201 cgccgccaat ggcggcggcg attggccgga caacgacgtc agatacgcgc ggctttcgct
     1261 ggccggcgcc gagatcggcg aaggcaaggc ggcgctcggg tggaagcccg atcttgtcca
     1321 tgcgcatgat tggcagaccg ggctgacgcc cgcttatctc agatggcggg gggccggcgc
     1381 ctcgtccgtc ttcaccattc acaacctcgc ccatcagggc ctgtacccgg ccgagcgctg
     1441 cgcggccctt ggcgcgcccg aatcggcttt ctcgatcaac ggcgtcgagt ttcacggccg
     1501 gctctccttc atgaaggccg gcgtgtacta tgccgatcat gtgacgacgg tcagccccaa
     1561 ttatgcgcgc gaaatcaccg aggaggcgca tggcggcggc ttgcatggct tgaccgccgg
     1621 gctggcggcg cagggtcggc tgaccggcat cgtcaacggc gtaggcccgg aatgggatcc
     1681 gtccaacgat ccggatatcg gccagccttt cgacaaggag gacacgtctg caaaggcctg
     1741 gatcaaggat cgtgtccgca ccggcctctg cctcgcccac cgtgacgggc cgctcttcgg
     1801 cgtcgtctcc cggctggttc atcaaaaagg tctcgatctg gtcgccgagg tcgccggcga
     1861 tatcgtcgaa cgcggcggcc agatcgcgat cctcggcgtc ggcgatccgg tcaccgagga
     1921 gatgctgcgc ggcgtcgtca gggcccatcc cgacgatatc gcgatgctca acggctataa
     1981 tgagccgatg gcgcgccgca tcatggcggc gagcgatttc tatctgatgc cgtcgcgctt
     2041 cgagccgtgc gggctgtcgc aaatgcacgc gcagctctat ggcgccttgc cgatcgtcca
     2101 tgcaaccggc ggcctcgtcg acacggtcga ggatggcgag accggcttcg tcttccgccc
     2161 gttcggcaag caaagcttcc tcgaagcgat cggacgcgcg ttttcgacgt acggcgcgcc
     2221 cggcgaaatc gaacgaatgc gccgcgcggc gatgagccgg cgctatggct atgacgatat
     2281 cgtcggcgaa tacataaaaa tctacgccca tcttaccggc aagccattgc ttcgcgtcgc
     2341 caataacagc gagacgccgc ggagcggcgg cgtttcggcg acgctgcgcg tcgcgcgcgc
     2401 gatgagcctg ctctgatctt ttgaaaagga ccggcggcgc gccaggctaa tcacggccgg
     2461 atctcgcacg atcgaccgat gaccctgacc cttcatggtt atttcgccgc aattcacatc
     2521 ccgaaaggaa ctttgccgcg gcttcgccgt tgtccgacgc caatgcgctc aacgcatggt
     2581 ctttcggaga cgttttcatg ggccgcgctc ttctcctctg gcttctcggc gtgccgattc
     2641 cgatcatcat cttgatcgcc tttc
//
LOCUS       DATGJW010000477         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1540844, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000477 DATGJW010000000
VERSION     DATGJW010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1540844"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            179..586
                     /locus_tag="VKU03_14325"
     CDS             179..586
                     /locus_tag="VKU03_14325"
                     /inference="COORDINATES: protein motif:HMM:NF027249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system HicB family
                     antitoxin"
                     /protein_id="HLJ72492.1"
                     /translation="MRYFVLIDGEAGDYGVAFPDLPGCVAMGDTVEGALANAADALRD
                     WIDLTEENGDSVPPPRPLEALRTDGEVARALSDGASLAAVPLVRETGKSIKANLSLDA
                     GLLAAIDEEAQRRKLTRSAFIELMARRMLSEVA"
     gene            847..1671
                     /gene="map"
                     /locus_tag="VKU03_14330"
     CDS             847..1671
                     /gene="map"
                     /locus_tag="VKU03_14330"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012591085.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HLJ72493.1"
                     /translation="MTFVDSAALHGRKSGHIKLHGPEAFAGMRKAGRLTAEALDLLAD
                     KVRPGVTTAALDRLCFEFAMDHHAYPASLYYRGYRKSICTSINHVVCHGLPDDKPLRD
                     GDILNIDVTLIVDGWHGDSSRMYVAGEAPRRAQRLIEITYESLMRGVAAVKPGGTTGD
                     IGAAIQTYAEGERCSVVRDFCGHGLGRLFHDEPNILHYGRPGEGVPLRPGMLFTIEPM
                     INLGRPQVKVLSDGWTAVTRDRSLSAQFEHTVGVTETGVEVFTYSPKGLDKPPYRV"
     gene            1668..2363
                     /gene="radC"
                     /locus_tag="VKU03_14335"
     CDS             1668..2363
                     /gene="radC"
                     /locus_tag="VKU03_14335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919433.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HLJ72494.1"
                     /translation="MTDGDEAAAPHYHGHRERLRQRLREAGAEAVADYELLELVLFRA
                     IPRRDVKPLAKSLIARFGSFAEAIAAAPERLAEIAGMTQGAIGEFKIVEASAQRFAKG
                     AAKKRLAIGSSSALVDYCRTAIAFAEREEFHVLFLDKRNGLIADEKLGAGTVDHTPVY
                     PREVVRRALELSASAIILVHNHPSGDPTPSGADVKMTQEIIAMGAPLGVAVHDHLIVG
                     RQGHASLKALRVI"
     gene            2444..2650
                     /locus_tag="VKU03_14340"
     CDS             2444..2650
                     /locus_tag="VKU03_14340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72495.1"
                     /translation="MPSAYFINDSAHWRKRGEEMRALAEEIRDPDAKARMLRIAADYD
                     KLASRAEQRASGAPSSTAPSADQK"
     gene            2719..3191
                     /locus_tag="VKU03_14345"
                     /pseudo
     CDS             2719..3191
                     /locus_tag="VKU03_14345"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017276125.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus and C-terminus; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
BASE COUNT          561 a         1037 c         1046 g          547 t
ORIGIN      
        1 ggcttgaaca ggaaggttgg gaactggcgc gccacggctc ggagcacgat ctttatcgcc
       61 gccccgagag gcccgtcatc gtcgtgccgc gccaccgcac gctttcgccg ggcgtcgccc
      121 gcgccatcgc caaaaccgcc ggctggctgt gagcgaaccg aaagacaagg aattgaagat
      181 gcgctatttc gttttgattg acggcgaggc tggcgattac ggcgtcgcct tccccgatct
      241 gcccggatgc gtcgcgatgg gcgacacggt cgagggggcg ctcgcgaacg ccgccgacgc
      301 tttgcgggac tggatcgatc tgacggaaga gaacggcgat tcggttccgc cgccgcggcc
      361 gctcgaagcc ctccgcactg acggggaggt ggcgcgggct ttaagcgatg gcgcaagtct
      421 tgccgccgtt ccgctcgtgc gcgaaaccgg gaagtcgata aaagccaatt tgtcgctcga
      481 tgcgggactg cttgccgcca tcgacgagga ggcccagcgt cgaaagctga cccgctcggc
      541 cttcattgag ctcatggccc ggcggatgct gagcgaagtc gcttgagaaa gccgcgccgg
      601 ggttgtcatt gcgcgcgcag tgaagcaatc catgagggcg gcggggctgc cgcaagcgcc
      661 gcatggattg cttcgctccg ctcgcaatga cggggcgctt gcttcggccg gcgaaggcct
      721 tacattaaag gtcagcgcac ccgtcattgc gagccaagcg aagcaatcca ctcgtagggc
      781 tgtgtcccat gaatggaggg cttcgcttcg cgcctcgtca atgacgattt cataagacct
      841 gacgtgatga ccttcgtcga ttccgccgca ttgcatgggc gcaaaagcgg ccacatcaaa
      901 ctgcatgggc ccgaggcttt cgcaggcatg cgcaaggccg gccggctgac cgccgaggcg
      961 ctcgatctgc tcgccgacaa ggtgcggccg ggcgtgacca ccgccgcctt ggaccgattg
     1021 tgcttcgaat tcgcgatgga tcatcatgcg taccccgcct cgctctatta tcgcggctac
     1081 cgcaagtcga tctgcacctc gatcaaccac gtcgtctgcc acggcctgcc cgacgacaag
     1141 ccgctccgcg acggcgacat tctcaacatc gacgtgacct tgatcgtcga cggctggcac
     1201 ggcgattcca gccgcatgta cgtcgccggc gaggcgccgc ggcgcgcgca aaggctgatc
     1261 gagatcacct atgaatcgct gatgcgcggc gtcgccgcgg tcaagccggg cgggacgacc
     1321 ggcgatatcg gcgccgcgat ccagacctat gccgagggcg agcgctgctc ggtggtgcgc
     1381 gatttctgcg gccatgggct cggccggctg ttccatgacg agccgaacat cctgcattac
     1441 ggccggcccg gcgagggcgt gcccttgagg cccggcatgc tgttcacgat cgaaccgatg
     1501 atcaatctcg gccggccgca agtgaaggtc ctctccgacg gctggaccgc ggtgacgcga
     1561 gatcgctcgc tctccgcgca attcgagcac acggtcggcg tcaccgagac gggcgtcgag
     1621 gttttcacct attcgcccaa ggggctggac aagccgccct accgggtatg acggacggcg
     1681 acgaagccgc ggcgccgcat tatcacggcc atcgcgaaag actgcgccag aggctgcgcg
     1741 aggcgggcgc cgaggcggtc gccgattacg agttgctcga actggttctc tttcgcgcca
     1801 ttccgcggcg cgacgtgaaa ccgctcgcca aaagcctgat cgcgcggttc ggctcattcg
     1861 ccgaggcgat cgccgccgcg ccggagcggc tcgccgaaat cgccggcatg acgcaaggcg
     1921 caatcggcga attcaagatc gtcgaagcga gcgcgcagcg cttcgccaag ggcgcggcga
     1981 agaagcgcct ggcgatcggc tcgtcgagcg cgctcgtcga ttattgccgc acggcgatcg
     2041 ccttcgccga gcgcgaggag tttcacgtcc tctttctcga caagcgcaac ggcctgatcg
     2101 ccgacgagaa gcttggcgcc ggcacggtcg atcatacgcc ggtctatccg cgcgaagtcg
     2161 tgcgccgcgc gctcgaactc tctgcctcgg cgatcatcct cgttcacaac catccgtccg
     2221 gcgatccgac gccgtccggc gccgacgtga agatgacgca agagatcatc gccatgggcg
     2281 cgccgctcgg cgtcgcggtg cacgatcacc tgatcgtcgg ccggcaaggc catgccagct
     2341 tgaaggcgct gcgggtgatt tgacccttct ccccttcagg gccttatttt ccgctggacg
     2401 ggcgcctttt ttaaagacaa tgatcggcgt tcaggggcgg gccatgccgt ctgcttattt
     2461 catcaacgat tctgcgcatt ggcggaagcg cggcgaggag atgcgcgcgc tggcggagga
     2521 aatccgcgac cccgacgcga aggcgaggat gcttcgcatc gccgccgatt acgacaagct
     2581 ggcgtcgcgg gccgaacagc gcgccagcgg cgccccgtca tcgacggcgc cgtcggccga
     2641 tcaaaaatag gcgggctgct tcaggacgcc gcgctgaagg cgatcggcga aatcggctct
     2701 cgcgctccag gggcgccctt cacccagccg atgacggagc tcttctcgat cacggcgacc
     2761 gatcagggac ggccgatcac cgatttcgcc catcgcctcg aatacgatcg actgaccagt
     2821 gacgcccaaa cggtgctcgc tcagctgacg ccgatcaggc gcgagattcg cagccgcgac
     2881 ggccgctggt acgatgttcg gctgcggccc tatcggaccg tcgaggaaaa gatcgacggc
     2941 gtcgtgatca cgtttgtcga cataaccgag cggctgcaaa ccgaggaggc gctgcggcag
     3001 gtcaatcggc tggtggaggt ctcccgcaat cccatattcg tttgggattt cgatggcggc
     3061 atccttggat ggaatcgcgg ttgcgaggag ctttacggct ataaccgcga ggaagcgctc
     3121 ggcaagcaga aggatcagct gctggccacc gccgttcccg gcgcctccgt cgccgggttg
     3181 cgcgcgcggc t
//
LOCUS       DATGJW010000478         3167 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1230312, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000478 DATGJW010000000
VERSION     DATGJW010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3167)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3167)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3167
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230312"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1697
                     /locus_tag="VKU03_14350"
     CDS             <1..1697
                     /locus_tag="VKU03_14350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266295.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease E/G"
                     /protein_id="HLJ72496.1"
                     /translation="RTKAEVKRDFEYLLRMWETVREKTLASTAPTLVYEEGSLIKRAI
                     RDLYNKDIDEVMVAGEEGYREAKEFMRLLMPSHARNVQLYRDPQPVFAKAGVEAQLDA
                     LFSNHVTLKSGGYLVINQTEALVAIDVNSGRSTREHNIEDTALRTNLEAADEVARQLR
                     LRDLAGLIVVDFIDMEEGRNNRAVERRIKDALKNDRARIQVGRISHFGLLEMSRQRIR
                     TGVLEGSTVLCPHCMGAGVVRSTASIALHVLRVLEDALIKSATHDLNVRTRSEVALYI
                     LNQKRSHLRDLERRFGVTIMVMADDSLAGATYHALERGEPAIGVKLGETEAREAPPPV
                     EEADEIEAEAEAEAEEESEAESQEEEAEGADEGAGPEPRAEGAPRREDGEGGRRRRRR
                     RRRRGGGREPGDVSGADAPQPTDAGLAAVAEIDGEQGARPRDEAERPRRGDFGRRSGG
                     WRRDGRDRNRPRRGEEQPETAAPSGDDSPALVYGDEAAPSAAFPQPQDGEAAVDAATP
                     PEREPAVEPQPSEPPAEFADAAHESEPSAAEPAEASPSRPKRSGWWQRARASIVGE"
     gene            1834..2592
                     /locus_tag="VKU03_14355"
     CDS             1834..2592
                     /locus_tag="VKU03_14355"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011441902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsbA family protein"
                     /protein_id="HLJ72497.1"
                     /translation="MKSFLALAAAAALIFGAVSAPAAEFTPAQKDEIGGVVKDYLIAH
                     PEVLREALDALDKHEKQAAAEAQKKIVADESSLLFSSPHQATVGNPNGGATLVEFFDY
                     NCHYCKGALPDIARLIKGDPNLKVVLKDFPVLGPDSVEAARVAIAVRNQLTGDKFWQF
                     HQKLLGMHGRVGKAEALAVAKDLGVDMDRLNKDMATPEARTSIEEVMRMADALQINGT
                     PSFVVGQEVVIGAVGYDQLKDKIDSVHKCGHAVC"
     gene            2863..>3167
                     /locus_tag="VKU03_14360"
     CDS             2863..>3167
                     /locus_tag="VKU03_14360"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphate phosphatase"
                     /protein_id="HLJ72498.1"
                     /translation="MTTLQAIVMAVLQGLTELFPVSSLGHAVVLPRLLHWPIDQKAPD
                     YLPYLVVMHLGTAIALFLYFWRDWLAFLISLINPANPRGSADRRIFLLVVLATIPAV"
BASE COUNT          556 a         1050 c         1075 g          486 t
ORIGIN      
        1 tgcgcaccaa ggccgaggtg aagcgcgact tcgaatatct cctgcgcatg tgggagacgg
       61 tgcgcgagaa gacgctcgcc tcgactgcgc cgaccctcgt ctatgaggag ggctcgctaa
      121 tcaagcgcgc cattcgcgac ctctacaaca aagacatcga cgaagtgatg gtcgccggcg
      181 aagaaggcta tcgcgaagcc aaggaattca tgcgcctgct gatgccgagc cacgctcgga
      241 acgtgcagct ctatcgcgat ccgcagccgg tcttcgccaa agccggcgtc gaggcgcagc
      301 ttgacgcgtt gttctccaat cacgtcacgc tgaaatcggg cggctatctc gtcatcaacc
      361 agaccgaagc tttggtcgcg atcgacgtca attccggccg ctcgacgcgc gagcacaata
      421 tcgaggatac cgcgctccgc accaatctcg aagccgccga cgaagtggcg cggcaattgc
      481 gcttgcgcga ccttgccggc ctgattgtcg tcgacttcat cgatatggag gaaggccgca
      541 acaatcgcgc ggtcgaacgg cgcatcaagg acgcgctgaa gaacgaccgc gcccgcatcc
      601 aggtcggccg catatcgcat ttcggcctgc tcgaaatgtc gcgccagcgc atccgcaccg
      661 gcgtgctgga aggctcgacc gttctctgcc cgcattgcat gggcgccggc gtcgtccgct
      721 cgaccgcctc gatcgcgctg catgtcctgc gcgtgctcga agacgcgctg atcaagagcg
      781 cgacccacga tttgaacgtg cgcacccgca gcgaagtcgc gctctacatc ctcaatcaga
      841 aacgctcgca tctgcgcgac ctcgagcgcc gcttcggcgt gacgatcatg gtcatggccg
      901 acgattcgct cgccggcgcg acctatcacg cgctcgagcg cggcgaaccg gcgatcggcg
      961 tcaagctcgg cgagaccgag gcgcgcgagg cgccgccgcc ggtcgaggag gccgacgaga
     1021 tcgaggccga ggcggaggcg gaggcggagg aagaaagcga agccgaaagc caggaagaag
     1081 aggccgaggg cgccgatgag ggcgccggtc cggagccgcg cgccgagggc gcgccgcggc
     1141 gcgaggacgg cgaaggcgga cgccgccgcc ggcggcgccg gcggcggcgc ggcggcgggc
     1201 gcgagccggg cgatgtcagc ggcgccgacg cgccgcagcc gaccgacgcc ggccttgccg
     1261 ccgtcgccga aatcgacggc gagcaaggcg cgcgcccgcg cgacgaggcc gaacggcctc
     1321 gccgcggcga tttcggccgg cgctccggcg gatggcggcg cgacgggcgc gaccgcaatc
     1381 ggccgcgccg gggcgaggag cagccggaga cggcggcgcc gtccggcgac gacagtccgg
     1441 ctttggttta cggcgacgag gcggcgccca gcgcggcttt tccgcaaccg caggacggcg
     1501 aggccgcggt cgacgccgcg acgccgccgg agcgcgagcc ggcggtcgaa ccgcagccaa
     1561 gcgagcctcc ggccgagttc gccgacgcgg cccatgagtc cgagccgagc gcggccgagc
     1621 cggccgaagc ctcgccgtcg cggccgaagc gctccggctg gtggcagcgg gcgcgcgcca
     1681 gcattgtcgg cgaatgaagg tcgaccccac gagggttaac tggggccgcg gcttggcctt
     1741 gcgcgaatct cgccgtattg aggcgctagt aggctgtgtg cttgtcgtcg gagccggcgc
     1801 gcgtcgcgcg ccggagcccc agaagaggtc gatatgaaaa gctttctcgc ccttgccgcc
     1861 gcggcggcgc tcatttttgg cgccgtctcg gcgcccgccg ctgaattcac gccggcgcaa
     1921 aaggacgaga tcggcggggt ggtgaaagat tacctcatcg cccatccgga agtattgcgg
     1981 gaggcgctcg acgcgctcga caagcacgaa aaacaggccg cggccgaggc gcaaaagaag
     2041 atcgtcgccg atgaatcgag ccttctcttc tcctcgccgc atcaggcgac ggtcggcaat
     2101 ccgaatggcg gggcgaccct ggtcgagttc ttcgactaca attgccacta ttgcaaaggc
     2161 gcgctgccgg atatcgcccg gctgatcaag ggcgatccca atctcaaggt cgtcttgaag
     2221 gatttcccgg tcctcggccc tgattcggtc gaagcggcgc gggtcgcgat cgccgtgcgc
     2281 aatcaattga ccggcgacaa gttctggcaa ttccatcaga agctgctcgg catgcatggt
     2341 cgggtcggca aggccgaggc gctcgccgtc gccaaggatc tcggcgtcga catggatcgg
     2401 ctgaataagg acatggcgac ccccgaagcg cgaaccagca tcgaggaagt catgcgcatg
     2461 gccgacgcgc tgcagatcaa cggcacgccg agcttcgtcg tcggccagga ggtcgtcatc
     2521 ggcgcggtcg gctacgacca gctcaaggac aagatcgaca gcgtgcataa atgcggccac
     2581 gcggtctgct gacgctgctc gcttcttcca gagagtacgg ccatacgtcc gccgcgccgc
     2641 gacctgcgcg aaacatcggc gccgttgagg cttaccctca ccccagccct ctcccgcaag
     2701 cgggagaggg ggcgggttga gcgcttcatc gacgtcagcg cgctatttgc gaggcgcgag
     2761 caccccttct ctcgcttgcg ggagaaggtg gcgcgaagcg ccggatgagg gtgagccgct
     2821 gcgacggcta agtttgcatt caacgagggg gctctcttcg ccgtgaccac attgcaagcc
     2881 atcgtcatgg ccgtgctgca aggcttgacg gaattgttcc cggtcagcag cctcggccat
     2941 gccgtcgtcc tgccgcgcct gctgcattgg ccgatcgacc agaaggcgcc ggactacctg
     3001 ccctatctcg tcgtcatgca tctcggcacg gcgatcgcgc tgttcctcta tttctggcgc
     3061 gattggctgg ccttcctcat ttcgctgatc aatccggcca atccgcgcgg atcggccgat
     3121 cgccgcatct ttctgctggt cgtgttggcg acgattccgg cggtgat
//
LOCUS       DATGJW010000479         3315 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_820896, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000479 DATGJW010000000
VERSION     DATGJW010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3315)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3315)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3315
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_820896"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..449)
                     /locus_tag="VKU03_14365"
     CDS             complement(<1..449)
                     /locus_tag="VKU03_14365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018455066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ72499.1"
                     /translation="MTTRRDLIRSAAAGAGAASLFGGFGFNPLIATAMAKEAGRATRP
                     LKAAFSNAGLQATWCAQGKQAAEYWGKLFNVEVTWFDGQLDAVKQRSAIDNMASQKWD
                     FVAIQAFGIGTLTAPVNKMIDAGIPVIDLDTLIAPLDTINVQAFLAP"
     gene            complement(721..2349)
                     /locus_tag="VKU03_14370"
     CDS             complement(721..2349)
                     /locus_tag="VKU03_14370"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893100.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016614 - oxidoreductase activity, acting
                     on CH-OH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase N-terminal
                     domain-containing protein"
                     /protein_id="HLJ72500.1"
                     /translation="MTQTYDYIIVGGGSAGCVLANRLSEDEDVSVLLLEAGGRDWHPF
                     YRMPAGFAKMTKGIGSWGWHTVPQRHMKNRILRFTQAKVLGGGSTINAQIYTRGNPAD
                     YDGWVGCKGWSYREVLAYFKRAEDNQRFVNDYHSYGGPLGVSMPIAPLPICDAYFRAA
                     QEMGIPYNDDVNGARQEGVGFYQLTQRNAQRSSAASAYLAPIRSRKNLAVRAKTMTTR
                     ILLENSRAVGVEILENNATTAIRAEREVLIAAGAFGSPKLLMQSGIGPADHLKSVGVA
                     VAHDLPGIGGNLQDHLDLFVIAECAGEHTYDNVAKWRRSAWAGLQYFLFKNGPVASSL
                     FETGGFWYADKTARWPDIQFHLGLGSGIEAGVARMRNPGVTLNSAFLRPLSRGSVRLA
                     SADPQAAPLIDPNYWAEPYDRKISIEGLKLARDIMRQKALKPFILAERLPGPEKATDD
                     DLFDYGCRHAKTDHHPVGTCRMGNDELAVVTPDLKLRGIEGLRVCDASVMPRIVSSNT
                     NAAAIMIGEKASDLVRGREPLPAVVFSTNQRVGA"
     gene            complement(2465..3235)
                     /locus_tag="VKU03_14375"
     CDS             complement(2465..3235)
                     /locus_tag="VKU03_14375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010911385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-ketoacyl-ACP reductase"
                     /protein_id="HLJ72501.1"
                     /translation="MSGRPVALVTGARRGIGRACGEKLAAAGFDIAATDLSLDAQCEE
                     ARASFAKAGADAVFFAHDVANVAAHAGLIDAVLKRFGRLDCLVNNAGRGAVQRGDLLD
                     LKPENFDAVLAVNLRGTLFLTQAAAKAMLQRQPADDPRSIVTITSVSAAMASPERAEY
                     CVSKAGLSMAMQNFALRLAPHGIAVFEVRPGIIRTDMTAPVSDRYAEAIGSGLVPMAR
                     WGEPDDVGDIVAGLASGRFAFATGSVISADGGLSAPRL"
BASE COUNT          564 a         1075 c         1117 g          559 t
ORIGIN      
        1 tccggggcga ggaacgcctg gacgttgatc gtatcgagcg gagcgatcag cgtgtccaag
       61 tcgatgacgg gaatgccggc gtcgatcatc ttgttgaccg gcgcggtcag cgtgccgatg
      121 ccgaatgcct ggatcgccac gaaatcccat ttctgcgagg ccatattgtc gatcgccgag
      181 cgctgcttga ccgcgtcgag ctggccgtcg aaccaggtga cctcgacatt gaacagcttg
      241 ccccaatatt cggccgcctg cttgccctgg gcgcaccatg tcgcctgcaa gccggcgttc
      301 gagaacgccg ccttcaaggg ccgcgtcgcc cggccggctt ccttggccat ggccgtggcg
      361 atcaagggat taaagccgaa gccgccgaac aggctggccg cgccggcgcc ggccgccgcg
      421 cttcggatca gatcgcgtct cgtcgtcatg atttcctccc gatagggccc ttctggcctg
      481 gacttgatcg agcacgctag catgacacga ctgccgcagc gacttcaagc aattgtctga
      541 ttttccagcc cctcggcccg ggcggcgggg agagggtggc cgcgaagcgg ccgggtgagg
      601 ggcggcgccg agattgccga ttagcccctt catcagacgc tgactttcga gtcctgcgca
      661 gcccctcatc cggcgcttcg cgccaccttc tccccgcaag cggggagaag ggtgggtacg
      721 tcaagcgccg acgcgctggt tggtcgagaa caccaccgcc ggcagcggct cgcggccgcg
      781 caccaggtcc gaggctttct cgccgatcat gatcgcggcc gcgttggtat tcgaggagac
      841 gatccgcggc atgacggacg cgtcgcagac gcgcaggcct tcgatcccgc gcaatttgag
      901 atcgggcgtc accacggcga gctcgtcatt ccccatgcgg caggtgccga cgggatgatg
      961 gtcggtcttg gcgtgccggc agccatagtc gaacagatcg tcgtcggtcg ccttttccgg
     1021 gccgggcagg cgctcggcga ggatgaaggg cttcagcgcc ttctgccgca tgatgtcgcg
     1081 ggcgagcttc aagccctcga tcgagatttt ccggtcataa ggctcggccc agtaattggg
     1141 atcgatcagc ggcgcagctt gcggatcggc gctggcgagg cgcaccgagc cgcgcgacaa
     1201 cgggcgcaaa aaggcggaat tcaaggtcac gcccggattg cgcatgcggg cgacgccggc
     1261 ctcgatgccc gacccgaggc cgagatggaa ctggatgtcc ggccagcgcg ccgtcttgtc
     1321 ggcgtaccag aagccgccgg tctcgaacag gctcgacgcg actgggccgt tcttgaacag
     1381 gaaatattgc agacccgccc aagccgagcg acgccacttg gcgacattgt cgtaagtatg
     1441 ctcgccggcg cattcggcga tgacgaaaag atcgagatga tcctgcagat tgccgccgat
     1501 cccgggcagg tcgtgcgcga ccgcgacgcc gaccgatttg agatgatcgg ccggcccgat
     1561 ccccgattgc atcagcaatt tgggcgagcc gaaggcgccg gcggcgatca gcacctcgcg
     1621 ctcggcgcgg atggcggtcg tcgcgttgtt ttcgaggatt tcgacgccga ccgcgcggct
     1681 gttttcgaga agaatgcgcg tcgtcatcgt cttggcgcgc acggcgagat ttttacggct
     1741 gcggatcggc gccagatagg cgctcgccgc cgacgagcgc tgggcgttgc gctgggtgag
     1801 ctggtagaag ccgacgccct cctgccgcgc gccgttgacg tcgtcattgt acggaatgcc
     1861 catttcctgc gccgcgcgga aataggcgtc gcagatcggc agcggcgcaa tcggcatcga
     1921 gacgccgagc ggtccgccat aggaatggta gtcgttgacg aagcgctggt tgtcctcggc
     1981 gcgcttgaaa taggcgagaa cctcgcggta gctccagcct ttgcagccga cccagccgtc
     2041 gtaatcggcg ggattgccgc gcgtatagat ttgcgcgttg atcgtcgagc cgccgcccag
     2101 caccttggcc tgggtaaagc gcaggatgcg atttttcata tggcgctgcg gaaccgtatg
     2161 ccagccccac gagccgatcc ccttggtcat tttggcgaag ccggccggca tccggtagaa
     2221 cgggtgccag tcgcgcccgc ccgcctcgag aaggagaacg gagacgtcct cgtcctcgct
     2281 gagacggttg gcgagcacgc agcccgccga gccgccgccg acaatgatgt agtcgtacgt
     2341 ctgggtcatc gttgaccgcc ttcgccccgc gtcattgcga gcgaagcgga agcaatccat
     2401 tcaaagggct gagtcttacg aatggattgc tccgtcgcta cgctcctcgc aatgacggct
     2461 atcatcacag cctcggcgcc gataggccgc catccgcgga aatcacgctt ccggtcgcga
     2521 aggcgaaacg gcccgaggcg aggcccgcca cgatgtcgcc gacatcgtcc ggctcgcccc
     2581 agcgcgccat cggaacgagg cccgagccga tcgcctcggc gtaccggtcc gaaaccggcg
     2641 ccgtcatgtc ggtacggatg attcccggcc gcacttcgaa cactgcgatt ccgtgcggcg
     2701 cgaggcgcag cgcgaaattc tgcatcgcca tggaaagccc ggcctttgag acgcaatatt
     2761 cggcgcgctc cggcgacgcc atggcggccg acaccgaagt gatcgtgacg atcgagcgcg
     2821 gatcatccgc cggctgtcgt tggagcatcg ccttggccgc cgcctgggtc agaaacaggg
     2881 tcccgcgcag gttgacggcg agaaccgcgt cgaaattctc cggcttcagg tcgagcaggt
     2941 cgccgcgctg aaccgcgccg cggccggcat tgttgacgag gcaatcgagc cggccgaagc
     3001 gcttgagcac ggcgtcgatc aggccggcat gagcggcgac attcgcaacg tcatgggcga
     3061 agaacaccgc gtccgccccg gctttggcga aagaggcgcg ggcttcctcg cattgcgcgt
     3121 cgaggctgag gtccgtcgcg gcgatgtcga agccggccgc ggcgagcttc tcgccgcagg
     3181 cgcggccgat gccgcgcctc gcgccagtga cgagcgcgac gggccggccg ctcatcgcga
     3241 aagctcttgg cgcacgcgtt cggcggcgcg aagcgcgaga gctgcgacgg tgagcgacgg
     3301 gttgaccgcg ccgga
//
LOCUS       DATGJW010000480         3417 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1131584, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000480 DATGJW010000000
VERSION     DATGJW010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3417)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3417)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3417
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1131584"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..822)
                     /gene="argF"
                     /locus_tag="VKU03_14380"
     CDS             complement(<1..822)
                     /gene="argF"
                     /locus_tag="VKU03_14380"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015933751.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0042450 - arginine biosynthetic process via
                     ornithine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HLJ72502.1"
                     /translation="MTAPAPAAPVRHFLDLSQVPARDLRDMLAASSAMKAARVKGAPP
                     AVRPLAGKTLAMIFDQPSTRTRVSFEKAMRELGGDAMMLTGAEMQLGRGETLADTARV
                     LSRYVDAIMIRILDQRALEELARYASVPVINGLTKQSHPCQAMADVMTFEEHRGPIRG
                     RKIAWSGDYNNVLSSWIDASARFEFSLDIACPRELQPPPLRLEAARALGADVKLSDDP
                     FAAVAGAAAVISDCWVSMGDEDEGRHNLLAPYQVNQRLMSEAAKDAIFMHCLPAHR"
     gene            complement(819..2018)
                     /locus_tag="VKU03_14385"
     CDS             complement(819..2018)
                     /locus_tag="VKU03_14385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007562412.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLJ72503.1"
                     /translation="MSSAIAPVYARADLAFEKGEGAWLVSTAGERYLDFGAGIAVASL
                     GYSRPHLVEALTRQGAKLWHTSNLFQIPEGERLARRLVEATFADLVFFANSGAEANEA
                     AIKMARKWQSANGRPERYRFITFEGAFHGRTLATIAAGGQAKYLEGFGPKVEGFDQIP
                     VADLDAVEKAIGPETGAILIEPIMGEGGVRVVPPSFLRALRALCDKHDLLLVFDEIQT
                     GIGRTGKLFAYEHAGIRPDIMTIAKGIGGGFPMGACLATADAAKGFTTGVHGTTFGGN
                     PLAMAVGNAVLDVVLAPGFIERVARLGLLLRQRLAELKDRHGAIIEEIRGEGLMIGLK
                     VKVPNADFAAAARQEKLLTIPAGDNTVRLLPPLIVSEEELAEGVRRLDAACARLEEPA
                     AQKGAAE"
     gene            2619..3140
                     /locus_tag="VKU03_14390"
     CDS             2619..3140
                     /locus_tag="VKU03_14390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GcrA family cell cycle regulator"
                     /protein_id="HLJ72504.1"
                     /translation="MWTDEKVELLRQLWLDGKSASQIAAELGEGVTRNAVIGKVHRLG
                     LSGRVKASAPAAPRIRQRPAAPRPSAPRSGGGFRGNTALALARETQVEPEPEFREDVV
                     IPMSLRVTIVELKEAMCRWPLGDPTSSEFRYCGIQTQGDGPYCLHHGRMAYQPAQDRR
                     RERERERRAVRIA"
     gene            complement(3247..>3417)
                     /locus_tag="VKU03_14395"
     CDS             complement(3247..>3417)
                     /locus_tag="VKU03_14395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HLJ72505.1"
                     /translation="AQLLDGVWGLSSEIDERTVDVHVGRLRKALSRGRERDPIRTVRG
                     AGYAFDETFGKM"
BASE COUNT          585 a         1123 c         1159 g          550 t
ORIGIN      
        1 gcgatgggcg ggcaggcaat gcatgaagat cgcgtccttg gccgcttcgc tcatcagtct
       61 ttgattgacc tgataaggcg cgagcagatt gtgccgccct tcgtcctcgt cgcccatcga
      121 tacccaacaa tcggaaatca ccgcggcggc gccggcaacg gccgcgaagg gatcgtcgct
      181 cagcttgaca tccgcgccga gcgcccgcgc cgcttcgagg cgtaaagggg gcggctgcaa
      241 ttcgcgcgga caagcaatgt cgagcgaaaa ttcaaaacgc gccgacgcgt cgatccagga
      301 cgagagcaca ttattgtaat cgccgctcca ggcgatcttg cggccgcgga tcgggccgcg
      361 atgctcttcg aaggtcatca catcggccat ggcttggcag ggatgggatt gcttggtcag
      421 gccgttgatg accgggaccg acgcatagcg cgccaattcc tccagcgcgc gctggtcgag
      481 aatgcggatc atgatcgcgt cgacatagcg cgagagaacc cgcgccgtgt cggcgagcgt
      541 ttcgccgcgg ccgagctgca tttccgcgcc ggtcagcatc atggcgtcgc cgccgagctc
      601 gcgcatcgcc ttctcgaagg agacgcgggt gcgcgtcgac ggctggtcga aaatcatcgc
      661 cagggtcttg ccggcgagcg gccgcacggc cggcggcgcg cccttgacgc gggccgcctt
      721 catcgccgag gacgccgcga gcatatcgcg caagtcgcgc gccggcactt gcgaaaggtc
      781 gagaaaatgc cggactggcg ctgcgggcgc tggcgcggtc attcggccgc gcccttttgc
      841 gcggccggct cttccagccg cgcgcaggcg gcgtcgagcc ggcgcacgcc ctcggccaat
      901 tcctcctcgc tgacgatcag cggcggcagc aagcgcacgg tgttgtcgcc ggcgggaatg
      961 gtcagcagct tttcctggcg cgccgccgcg gcgaaatcgg cgttgggaac tttgactttc
     1021 aggccgatca tcaggccctc gccgcggatc tcttcaatga tcgcgccatg ccggtctttc
     1081 aattcggcga ggcgctggcg cagcaggagg ccgagccggg cgacccgctc gataaagccc
     1141 ggggcgagca ccacgtcgag cacggcgttg ccgaccgcca tggccagcgg gttgccgccg
     1201 aacgtcgtgc cgtgaacgcc ggtcgtgaag cctttggccg cgtcggcggt ggcgaggcag
     1261 gcgcccatcg gaaaaccgcc gccgatcccc ttggcgatgg tcatgatgtc gggccgaatg
     1321 ccggcatgtt cataggcgaa gagcttgccg gtgcggccga tgccggtctg gatttcgtcg
     1381 aagacgagca acaggtcgtg cttgtcgcaa agcgcgcgta aagcccgcag gaaggacggc
     1441 ggcacgaccc gcacgccgcc ctcgcccatg atcggctcga tcaggatcgc gccggtttcc
     1501 gggccgatcg ccttttcgac ggcgtcgaga tcggcgacgg gaatttggtc gaaaccctcg
     1561 actttcgggc cgaagccttc gagatatttg gcttggccgc cggcggcgat cgtcgccagg
     1621 gtgcggccat ggaaagcccc ttcgaacgtg atgaagcgat agcgctcggg ccggccattg
     1681 gccgactgcc acttgcgcgc catcttgatc gccgcctcgt tggcctcggc cccggaattg
     1741 gcgaagaaga cgagatcggc gaaggtcgcc tcgaccaggc gacgggcgag cctttcgccc
     1801 tcgggaatct gaaagagatt ggacgtgtgc caaagcttcg cgccttggcg ggtcaaggcc
     1861 tcgacgagat ggggacgcga atagccgagc gaggccacgg cgatgcccgc cccgaaatcg
     1921 agatagcgct cgccggcggt cgagaccagc caggcgccct cgcccttttc gaaggcgaga
     1981 tcggccctcg catagaccgg cgcgatcgcg gaagacacgt ctgctaagct ccaggttgcg
     2041 caaacaaaaa cgccgccccg ctgggacggc cgctcaacgg gtcggatcat aacgcgcgcc
     2101 ggccttttgg gtcaaatggt tgcggcagct ccttctcccc gcggagcgca gggctgtccg
     2161 ctctgcaaac tcttgcgaaa agttaagggc gcaacaacgc aaatcccaat cgcgccgcca
     2221 tcgccgcggg cgttggaagt taagtcgcca ttcagcggaa ccgcttgccg gcgatttttc
     2281 ccggacagcc ccgcgcggag cggggagaag ggtacgattg gtcgcggctc gcgccgcatc
     2341 gctcatcctc tgttgaaaac agccgccgct gttgaaaaca gccgggccgc ccaaggccgg
     2401 actcttgcgc gcgattcaac gcatagtgta gattcctttt cgtcggctac gagatgtcgt
     2461 ggggccgatt ttctagagag agcgtgaagt gcggagcgtt cgtcggcgcg accgaacgag
     2521 cgggggcgcc ggattccgtc caacgcgtcg aaggcgcgac ttaaggcgcg cctctcaggg
     2581 ccggccatgt ttggagcgcc ggcggaaacg aggccaaaat gtggactgac gagaaggtcg
     2641 agcttttgcg gcaattatgg ctcgacggaa agagcgccag ccagatcgcc gccgagctcg
     2701 gcgagggcgt gacgcgcaac gccgtgatcg gcaaagtgca tcgactcggt ctctccggcc
     2761 gggtcaaggc cagcgcgccg gccgcgccgc gcatccggca gcggccggcg gcgccgcgcc
     2821 catccgcccc gcgcagcggc ggcggcttcc gcggcaacac cgctttggcc cttgcgcgcg
     2881 agacgcaggt cgagccggag ccggaattcc gcgaggacgt cgtcatcccg atgtcgctgc
     2941 gcgtgaccat cgtcgaattg aaggaggcga tgtgccgctg gccgctcggc gatccgacct
     3001 cgtcggaatt ccgctattgc ggcatccaga cgcagggcga tgggccctac tgcctccatc
     3061 acgggcggat ggcctatcag ccggcgcaag accggcgccg ggaaagagag cgtgagcgcc
     3121 gcgccgtgcg gatcgcgtag ggccgcatct tctccgggcc gcaggctgcc gcgccgccag
     3181 aaaagcgcgg cgtcctacgg acccgttgct ttggcgcttc gctcggcgcg atgacgagat
     3241 tgcgcgctac atcttcccga acgtctcgtc gaacgcgtag ccggcgccgc gaacggtgcg
     3301 gatcgggtcg cgctccctgc cgcgcgacag cgccttgcgc agtcgcccga catgaacatc
     3361 gaccgtgcgc tcgtcgatct cgctcgacag gccccagacg ccgtccagca attgcgc
//
LOCUS       DATGJW010000481         3238 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_301771, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000481 DATGJW010000000
VERSION     DATGJW010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3238)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3238)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3238
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_301771"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(165..1517)
                     /locus_tag="VKU03_14400"
     CDS             complement(165..1517)
                     /locus_tag="VKU03_14400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009756256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLJ72506.1"
                     /translation="MTQADLIAEPGGGVPVVARSDLVVVGGGPAGIAAAISGARNGLS
                     VTLVERYPYLGGLASGGMVLVLDDMCNGAEITVRGLCGEMIERMERIGLCVAPPEEDR
                     RSDEALWRKWSRWGVFDFHSHQKPQPICYAAAFDPDGFKRVANKMVAEAGVNLRLHSW
                     FSRSIVEGGAIRGVVCDTKAGRQAIMGSLVIDATGDLDVAASAGAPFTHGSYIVTTVF
                     RLGGVDIETAERFEYEQPEAFNTIDRAIKKLMGGSWDKWWLRTPLPGIVWCNCPHMVG
                     YDGIKVEDLTRGDFEGRDRIEAVVTYARAHLPGFENAFVVDVAPQIGVRQTRLLDGVY
                     VVTKEDVVQRRHFADSVARGRDYYYPYRSMLPRNVGGLIVAGRHYSATSSAQRISREI
                     PPCMAMGEAAGVAAALALQDGVGVEAINVGKAQAILRRQGADPGDAPAPNATLDEAAA
                     "
     gene            complement(1569..2417)
                     /locus_tag="VKU03_14405"
     CDS             complement(1569..2417)
                     /locus_tag="VKU03_14405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402831.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72507.1"
                     /translation="MSAQTLEATPAEPALGEDDAAQRRFEAQRRYLAWRRKLLPFAAI
                     VLLLVLWEAGVAIFHVRPFIAPAPSAVAALLVKKIGLLLQNLAPTAVEAVSGFVLGNL
                     AAIMIATVFVYRRTVEEAFFPIAVMVHTIPVMAYAPILVLLLGNGIEAKIAIAAMICF
                     FPTLVNMTRGLRSVTPQQMELMRILSASPREIFFTLRLKNALPYLFSALKIAAATSVI
                     GAIVGEWIGATTGIGALIIQATYNFDSPLLYATIVVGSAFSALFFAVVSWAERFFLKW
                     NVGEAA"
     gene            complement(2417..3217)
                     /locus_tag="VKU03_14410"
     CDS             complement(2417..3217)
                     /locus_tag="VKU03_14410"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402832.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72508.1"
                     /translation="MSLAALKQDDTPAPPLVSLRGVSMRFATERGVITALERIDLDVE
                     DGGFLTLLGPSGCGKSTLLRVVSDLLEPSAGAVKVLGVAPSVARARRDIGFVFQDPAL
                     LPWRTALQNVELPLQVGGGKGRPGRRTPRDLLQLVGLSGWENALPHELSGGMRQRVAI
                     ARALVNDPKLLLMDEPFGALDEITRDRLNEELLRVWEETGATVLFVTHSIYEAAFLGQ
                     KVLLLAARPGRVREIVPVPLPSPRRLALRETPAFVELVAHLRRVLETC"
BASE COUNT          607 a         1074 c         1086 g          471 t
ORIGIN      
        1 gaccctctcc cgcaagcggg agagggggcg cgccgagccc gtcatcagcc gccgcgctgt
       61 agccgcggtc gctgacggcg gcgacatccc ttctcccgct tgcgggagaa ggtggcgcga
      121 agcgccggat gagggtaagc cgcggtcgcc gaccgcggct acggtcaagc cgccgcttcg
      181 tcgagcgttg cgttcggcgc cggggcgtcg ccgggatcgg cgccttgccg acgcaagatc
      241 gcttgcgcct tgccgacatt gatcgcctcg acgccgacgc cgtcttgcaa cgcgagcgcg
      301 gcagcgacgc cggccgcctc gcccatcgcc atgcagggcg ggatttcgcg cgagatgcgc
      361 tgggccgaag aggtcgccga ataatggcgc ccggcgacga tcaagccgcc gacattgcgc
      421 ggcagcatcg aacggtacgg ataataataa tcgcggccgc gggcgacgga atcggcgaaa
      481 tgccggcgct ggacgacatc ttctttcgtc acgacataga cgccgtcgag gagccgcgtc
      541 tggcgcacgc cgatctgcgg cgcgacatcg acgacaaagg cgttttcgaa gccgggcaga
      601 tgcgcgcggg cgtaggtcac gaccgcctcg atccggtcgc ggccttcgaa atcgcccctc
      661 gtcaaatcct cgaccttgat cccgtcatag ccgaccatat gcgggcaatt gcaccagacg
      721 attcccggca gcggcgtgcg cagccaccac ttgtcccacg agccgcccat cagcttcttg
      781 atcgcgcgat cgatcgtgtt gaacgcttcg ggctgctcat attcgaagcg ctcggccgtc
      841 tcgatgtcga cgccgccgag ccggaacacc gtcgtcacga tatacgagcc atgcgtgaac
      901 ggcgcgccgg ccgaggcggc gacgtcgaga tcgccggtcg cgtcgataac gagcgagccc
      961 atgatcgcct ggcggcccgc cttggtgtcg cagacgacgc ccctgatcgc gccgccttcg
     1021 acgatcgagc gggaaaacca ggaatgcagg cgcaggttga cgccggcctc ggcgaccatc
     1081 ttgttggcga cgcgcttgaa cccgtcggga tcgaacgcgg cggcgtagca gatcggctgc
     1141 ggcttttgat gcgaatgaaa atcgaacacg ccccagcgcg accatttgcg ccacagcgcc
     1201 tcgtcggagc ggcgatcctc ctccggcggc gccacgcaga ggccgatcct ctccattcgc
     1261 tcgatcattt cgccgcatag gccgcgcacc gtgatctcgg cgccgttgca catatcgtcg
     1321 agcacgagga ccatgccgcc cgaagcgagg ccgccgagat agggatagcg ctcgacgagc
     1381 gtcacggaga ggccgttgcg cgcgccggaa atagcggcgg cgattcccgc cggaccgccg
     1441 ccgaccacga caaggtcgga acgcgcgacg accggaacgc cgccgccggg ctcggcaatg
     1501 aggtcggctt gtgtcatggc gtttgttcct cgaatcctct aacttcgctc gcaatgacgg
     1561 cgtcccgctc atgccgcctc gccgacattc catttaagga agaaacgctc ggcccacgag
     1621 acgacggcga agaacagcgc ggaaaaggcc gagccgacga cgatcgtcgc atagaggagc
     1681 ggcgaatcga aattgtacgt cgcctggatg atcagcgcgc cgatgccggt cgtcgcgccg
     1741 atccactcgc cgacgatagc gccgatcacc gaggtcgcgg cggcgatttt cagcgccgag
     1801 aacagatagg gcagcgcgtt tttcaagcgc agcgtgaaga agatttcgcg cggcgacgcc
     1861 gacaggatgc gcatcaactc catctgttgc ggcgtcacgc tgcgcaggcc ccgcgtcatg
     1921 ttgacgaggg tcggaaagaa acaaatcatc gccgcgatgg cgattttcgc ctcgatgccg
     1981 ttgccgagca gcaaaaccag gatcggcgca taggccatga ccgggatggt atgaaccatc
     2041 acggcgatcg gaaagaacgc ctcttcgacc gtgcgccgat agacgaagac tgtcgcgatc
     2101 atgatagcgg cgagattgcc gaggacgaaa ccggatacgg cttcgacggc ggtcggcgcg
     2161 agattttgta agagcaggcc gattttcttg acgagaagcg ccgcaaccgc gctcggcgcc
     2221 ggcgcgatga acggcctgac atggaaaatc gcgacgcccg cctcccacaa gacgagcagc
     2281 agcacgatgg cggcgaaggg cagcagcttg cgccgccagg cgagatagcg gcgctgcgcc
     2341 tcgaaacgac gctgcgccgc gtcgtcttcg ccaagagccg gctcggccgg cgtcgcctcg
     2401 agcgtttgcg ccgacatcag caggtctcca gcacgcgccg caaatgggcg acaagctcga
     2461 cgaaagcggg cgtctcgcgc agcgcgagcc ggcgcgggct cggcaggggc acgggaacga
     2521 tctcgcgcac ccggccgggg cgcgccgcca gaagcaggac cttctggccg agaaaggccg
     2581 cctcgtagat cgaatgggtg acgaacaaga ccgtcgcgcc ggtttcttcc cagacgcgga
     2641 gcagctcctc gttgaggcga tcgcgggtga tctcgtcgag cgcgccgaaa ggctcgtcca
     2701 tcaggaggag cttcggatcg ttgaccagcg cgcgggcgat ggcgacgcgc tggcgcatgc
     2761 cgccggaaag ctcatgcggc agcgcgttct cccagccgga aaggccgaca agctgcaaaa
     2821 gatcgcgcgg cgtgcgccgt ccgggccgtc ccttgccgcc gccgacttgc agcggcaatt
     2881 cgacattttg cagcgccgtg cgccacggca gcagcgccgg gtcctggaag acgaaaccga
     2941 tatcgcgccg cgcgcgggcg acggacgggg cgacgccgag cactttgacg gcgccggcgc
     3001 tcggctcgag caaatccgag acgacgcgca gcagggtcga cttgccgcaa cccgaagggc
     3061 cgagcaaggt caggaagccg ccgtcctcga catcgagatc gatccgctcg agcgcggtga
     3121 tcacgccgcg ctcggtggcg aagcgcatgc tgacgccgcg gagagagaca agcggcggcg
     3181 caggcgtgtc gtcctgtttc agcgcggcga gcgacattcc tggattctcc ctcacccg
//
LOCUS       DATGJW010000482        20210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_922297, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000482 DATGJW010000000
VERSION     DATGJW010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 20210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 20210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..20210
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922297"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..843)
                     /gene="tyrS"
                     /locus_tag="VKU03_14415"
     CDS             complement(<1..843)
                     /gene="tyrS"
                     /locus_tag="VKU03_14415"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383037.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HLJ72509.1"
                     /translation="MAETFRPRSDFLATLYERGFVHQCSDPAGLDAKATDGSLIAYIG
                     FDCTAPSLHVGSLVQIMLLHWLQKCGGKPLALMGGGTTMVGDPSGRDESRKLLTVQQI
                     EANKAGIRKAFSRFMTFGESKTDAVMPDNAEWLAKLNYIEFLREVGRHFSVNRMLAMD
                     SVKLRLSRDQELSFLEFNYMCLQAYDFVELHRRYGCVLQMGGSDQWGNIVTGVDLGRR
                     LGTAQLYALTSPLITLASGAKMGKTAAGAVWLDADLLSPYDYWQFWRNTEDADVGRFL
                     RLFTT"
     gene            932..1915
                     /gene="thiL"
                     /locus_tag="VKU03_14420"
     CDS             932..1915
                     /gene="thiL"
                     /locus_tag="VKU03_14420"
                     /EC_number="2.7.4.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009030 - thiamine-phosphate kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine-phosphate kinase"
                     /protein_id="HLJ72510.1"
                     /translation="MPAPLSEDDLIARFFAPMAAPEGLGLKDDAALLAGRPGDLVLTK
                     DALVGGVHFFADDPPGAIARKALRVNLSDLAAKGAEPVGFLLALALPGGWTEDWLAAF
                     AAGLADDAATYRCPLFGGDTVKTPGPLMVSITAFGRIEAGRMARRDGVKPGDRLYVSG
                     TIGDAALGLALRLGAPALAGLEAASRAFLLDRYLLPRPRTALASAMRQFAHGGMDVSD
                     GFVGDLTKMLRVSGVSGRIDLSHLPLSPAAEAALAAENGLLETIATGGDDYEILAAIE
                     PAEAPAFERAAEEAGVRITRVGEAVAGKGAPQFTAPGSRAIVFQRGSFSHF"
     gene            1928..2737
                     /locus_tag="VKU03_14425"
     CDS             1928..2737
                     /locus_tag="VKU03_14425"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018011712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008934 - inositol monophosphate
                     1-phosphatase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006020 - inositol metabolic process
                     [Evidence IEA];
                     GO_process: GO:0046855 - obsolete inositol phosphate
                     dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inositol monophosphatase family protein"
                     /protein_id="HLJ72511.1"
                     /translation="MRLDATALQNRLLAACAVAREAGALARRRFLDRTSFTVGFKGPQ
                     DFITEVDGEVEKLIRARLIGIFEQDGFIGEEGGAAPGAEGAPSWVVDPIDGTANFARG
                     APFYCVSIALIRNREIEVGVIYDPMLDELFAARRGAGAVVNGVPLKVSETASLARATV
                     EVGWNMRFGAEDFLAVMGRIVATGAGVVRAGSGALGLAYIAAGRRDGYVETHIKAWDC
                     LAGVLLVREAGGYASDFLAGEGLSQGNPLIACAPGVKEALIEAAAIKDLAL"
     gene            2849..3739
                     /locus_tag="VKU03_14430"
     CDS             2849..3739
                     /locus_tag="VKU03_14430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265391.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldose 1-epimerase family protein"
                     /protein_id="HLJ72512.1"
                     /translation="MTDLVRLSAGEASLAIAPEGAEAREWRIAGRDLLWSGDPAFWNQ
                     ISPILFPVVGWTRDGARVDGRFYPLRLHGFAAAQLFALDKQEPAKARFVLTDNDETRA
                     VYPFAFRLAVEYSLTPTRLEIALEMENRGSGPAPYAFGLHPGFRWPFDGAGRDGCRVV
                     FEKPESGDIPVLAPGGMVSPRRRPIPMKGNVLDLNDELFAKDALCFLSPASRWLRFVG
                     ASGAAIEMALGDFPHAALWMRPGAPFLCMEAWTGYSDPEGFAGDLFEKPAMRVVAPGE
                     KQRCVAAYTYLPPSAAGASS"
     gene            3736..4692
                     /gene="folD"
                     /locus_tag="VKU03_14435"
     CDS             3736..4692
                     /gene="folD"
                     /locus_tag="VKU03_14435"
                     /EC_number="1.5.1.5"
                     /EC_number="3.5.4.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional methylenetetrahydrofolate
                     dehydrogenase/methenyltetrahydrofolate cyclohydrolase
                     FolD"
                     /protein_id="HLJ72513.1"
                     /translation="MNQAVTSILKPSAERALIIDGKAASQALLARLAEEARRLIAAGV
                     RPGLSVVLVGEDPASQVYVRSKGRVASELGFRSEQHDLSPATSEEDLLALVERLNADP
                     GVHGVLVQLPLPKHIRSARILEAIAPHKDVDGFHPINVGLAAIGEAKRALIPCTPAGA
                     MILIEKACAALGESLAGKEAVVIGRSNIVGKPLARLLLAANCTVTVAHSRTRDLAAVA
                     RRADLLIAAVGQPELVRGDWVKPGAIVIDVGVNRIPGEGLNASGQPKTRLVGDVAFAE
                     AAQRAAAITPAPGGVGPMTIAMLMANTLIAACRAQGLEEPKL"
     gene            4954..7095
                     /locus_tag="VKU03_14440"
     CDS             4954..7095
                     /locus_tag="VKU03_14440"
                     /EC_number="3.6.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011580480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009678 - diphosphate hydrolysis-driven
                     proton transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sodium-translocating pyrophosphatase"
                     /protein_id="HLJ72514.1"
                     /translation="MTIVLILIILSGLLSVAYGGFTISQLMAADAGTKRMQEISGAVR
                     EGAQAYLRRQYTTIAGVGVIVFIGLALLLSIDVAIGFLIGAVLSGAAGFIGMNVSVRA
                     NVRTAQAATKSLGAGLEIAFKSGAVTGMLVAGLALLGVAFYYWILTGPLGLTPASRTV
                     IDALVALGFGASLISIFARLGGGIFTKGADVGADLVGKVEAGIPEDDPRNPATIADNV
                     GDNVGDCAGMAADLFETYAVTVVATMVLASIFFAGGASLVPAMLYPLAICGCCIVTSI
                     IGAQFVQLGANNSIMGALYKGLIVTGVLSILGLAVATQFVIGWGDIGTVHDKAVTGAN
                     LFICGIVGLIVTGLIVVITEYYTGTGKRPVVSIAQASVTGHGTNVIQGLAVSLEATAL
                     PALVIIGGIIASFQLAGLFGIAVAVTTMLGLAGMIVALDAFGPVTDNAGGIAEMAGLP
                     KEVRQSTDALDAVGNTTKAVTKGYAIGSAGLGALVLFAAYSNDLTYFASNPDKFPYFK
                     DIGQVSFELSNPYVVAGLIFGGLIPYLFGGIAMTAVGRAAGSVVLEVRRQFREKPGIM
                     QGTDRPDYGRAVDMLTKAAIREMIIPSLLPVLAPFVVYFGVLFISGEKKEAFAALGAS
                     LLGVIVNGLFVAVSMTSGGGAWDNAKKSFEDGFTDKDGVKHLKGSDAHKASVTGDTVG
                     DPYKDTAGPAVNPAIKITNIVALLLLAILAH"
     gene            7355..8173
                     /locus_tag="VKU03_14445"
     CDS             7355..8173
                     /locus_tag="VKU03_14445"
                     /inference="COORDINATES: protein motif:HMM:NF015438.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MOSC domain-containing protein"
                     /protein_id="HLJ72515.1"
                     /translation="MRLVQIRRYPVKGLRGYSLQRAEVQRIGLAGDRRWMIVDEAGKF
                     VTQRQCPKLAQIDASPTARGVALRHATHGAIEIDVPDARAPIETVVVWRDAVQARVAE
                     SAGAYLSAFLEKPVRLVFLHDASERRVDPAFGRAEDRVSFADGFPMLITSAASLADLN
                     GRLPQAIGMDRFRPSLVIDGAPAWAEDTWRKVRIGGLSFRIAKPCSRCAVPTLDPLTG
                     EALDGNEPIRTLGAFHRAANGGIIFGQNAIPDDVGALQVGDPVEIVETGSSNLL"
     gene            complement(8279..8740)
                     /gene="bamE"
                     /locus_tag="VKU03_14450"
     CDS             complement(8279..8740)
                     /gene="bamE"
                     /locus_tag="VKU03_14450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589698.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane protein assembly factor BamE"
                     /protein_id="HLJ72516.1"
                     /translation="MGSSGALRTGAARVLCVLSLATSLCGCLGYDGDFDRGYQIDEKT
                     YSQVKIGATTKQEVLSLMGTPSTTSTVGGDAWYYIGQKMHRALAFMPVKMEDQNVLAI
                     YFAPKTGKVERIANYGLKDGEIFDFVSRTTPTGGQEPSFLRNMFNNLFRFS"
     gene            8833..9384
                     /locus_tag="VKU03_14455"
     CDS             8833..9384
                     /locus_tag="VKU03_14455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome C chaperone family protein"
                     /protein_id="HLJ72517.1"
                     /translation="MVLARWRARRANRDLIEQLHGEIVAAARRPALYADFHAPDTLDG
                     RFEMIALHAGLILRRLHAISGLGDEIAQDLADCLFRHFDVALREIGVGDTSIPKRMKR
                     MAEAFYGRNKAYAEGLAAPGNDRLAEALARNVYGAASLAEAPYAPALARFARDCAAAL
                     DRLGLEDFAAGRIAFPEPSAAQP"
     gene            9381..9920
                     /locus_tag="VKU03_14460"
     CDS             9381..9920
                     /locus_tag="VKU03_14460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018390989.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HLJ72518.1"
                     /translation="MTPPFSRPVRVEAIPRDGLETRIDADAGERAALAAFNGLPAVAA
                     LGAAFSLKHGGGGTILVRGQLTAEVTQTCVVTLEPFDASISAPIDLRFAPAGEASAGP
                     DSAASEAEAMLVVGEEDEPDPIVDGVIDLGAVASEFLTLNLDPYPRKPGAAFEAPASG
                     GDGASDSPFSVLAERKKDA"
     gene            10127..11188
                     /gene="plsX"
                     /locus_tag="VKU03_14465"
     CDS             10127..11188
                     /gene="plsX"
                     /locus_tag="VKU03_14465"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012386285.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HLJ72519.1"
                     /translation="MNKDVRIALDAMGGDYGPKVVAPAASIALTRRPDVSYLFFGDEK
                     LIRPLVEADANLKRKTRIIHTDVAVKMSDKPSQALRAGRRSSSMWLALDAVKKGEADV
                     AVSAGNTGALMAMSKICLRTMPQIDRPAIAAMWPTLRGESIVLDVGATIGADAQHLID
                     LAVMGSAMARIVFDIERPTVGLLNVGVEEIKGLEEVKAAGRALREANFPNLDYRGFVE
                     GDDLGKGTVDVIVTEGFTGNIALKTAEGTARQIAQYIRDAMRHSFFARIGYVFARRAF
                     EALRDKLDPSKVNGGVFLGLDGVVIKSHGGADAEGYAGAIEVGYDMVRHELLGKTRDM
                     IAQANESRAQPPPMLAAES"
     gene            11222..12199
                     /locus_tag="VKU03_14470"
     CDS             11222..12199
                     /locus_tag="VKU03_14470"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012563028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HLJ72520.1"
                     /translation="MKTLRSVVRGTGSYLPARIMSNAELSTMVETSDEWIQQRSGIRE
                     RHIAAEGETTSILATRAAEAALANAGLTGADIDLVIVATSTPDYTFPAAATQVQANLG
                     MRHGVAFDLQAVCSGFVFAVATADKFLSSGSHQRALVIGAETFSRILDWKDRTTCVLF
                     GDGAGAIVLEAQESDGRLGSPGVITAQLRSDGAHRAKLYVDGGPSSTQTVGHLRMEGR
                     EVFRHAVGMVTDVIKAAFAAADVTAADIDWFVPHQANRRIIDASAEKLGIAPEKVVKT
                     VDQHGNTSAASIPLALDIACRDGRIKKGDLVMIEAMGGGFTWGAALIRW"
     gene            12324..13259
                     /locus_tag="VKU03_14475"
     CDS             12324..13259
                     /locus_tag="VKU03_14475"
                     /EC_number="1.14.19.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020181427.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006629 - lipid metabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fatty acid desaturase"
                     /protein_id="HLJ72521.1"
                     /translation="MGIADFPQSTRRVEWPTLGLAILIYGLWFGATFFHDRLPLWLLT
                     PVAAWTVAWHMSLQHEIIHGHPTRLRWLNSLIGAWPLALWLPFEDYRHTHLTHHRDER
                     LTDPLDDPESFYWRQEDWDALGPIGRALVRLQATLLGRMVIGPAWSCAKLWQREIQRL
                     ARGEKRALRDVALHLAEMAAVLAWVVGVCKMPIVEYVLCFAYAGTSLALVRSFAEHRA
                     ESLVERRTAIVERSWLLGPLFLFNNLHLAHHMRPAMPWYKLPGWYAANRAALIERNGG
                     LLYVTYFDVLRRYLLRPHDQTLHPLQGEDGASRAA"
     gene            13442..14248
                     /locus_tag="VKU03_14480"
     CDS             13442..14248
                     /locus_tag="VKU03_14480"
                     /inference="COORDINATES: protein motif:HMM:NF024376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PhnD/SsuA/transferrin family substrate-binding
                     protein"
                     /protein_id="HLJ72522.1"
                     /translation="MDALVASLGMYDFPFLAEANRRLWAAIGGSLRAAGIDAPVALDR
                     SEPCEVWANPRLVFGQTCGYPFVTALRDRVSLLATPIYSFEGCEGPNHCSFVIARAGS
                     RGRSLAGFQGARAALNARDSNTGMNLFRALIAPLARGRPMFSQALVTGSHAASLEAVA
                     EGVADIAAIDCVTYGLLRRGRPDLAEKVVVIARSQASPGLPFVISAALGEPLAAAVRV
                     ALYAALNDPGLAEAREALGLKGAAILDETAYRKIAEIERGAVALGYPELA"
     gene            14379..14687
                     /locus_tag="VKU03_14485"
     CDS             14379..14687
                     /locus_tag="VKU03_14485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72523.1"
                     /translation="MPTLSLLRITATAVALTVSAAAFAQANEEGNAPENNAAATAPKM
                     HMTHRMRHHHHYRRYTRHRSSGSQRMGNGSNGTNPNNGVSGATSGEGNAQTTNPQSNQ
                     "
     gene            complement(14734..16014)
                     /locus_tag="VKU03_14490"
     CDS             complement(14734..16014)
                     /locus_tag="VKU03_14490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycerate kinase"
                     /protein_id="HLJ72524.1"
                     /translation="MTADHRALLKRMFDAAVAAAAPAHCVPSHLPPPPKGRTIVVGAG
                     KAAAAMAAAVEAHWRGPLEGLVVTRYGHGAPTTRIKVVEASHPVPDEAGPAAARAILD
                     LVSGLTADDLVLALISGGGSALLALPGGAITLADKQQINRALLKSGAHIGEMNCVRKH
                     LSAIKGGRLAAAAAPARVVSLIISDVPGDDLSVIASGPTVADATTLADARRVLEKYKI
                     EVSPAVRQHLSDPANETPKPGDLRLANVTNLLIATPQSSLEAAAKIARDAGFAPVILG
                     NAIEGEARECGIVHAGIARQCADYGQPAAPPCAVISGGETTVTVRGKGRGGRNAEFLL
                     SLAVALDGHLGISALAADTDGIDGSEDNAGAIIGPDTLARAARLGLDLKAMLADNDAY
                     TAFSALGDLLITGPTRTNVNDFRAILAVKEEPAA"
     gene            complement(16062..16952)
                     /gene="glxR"
                     /locus_tag="VKU03_14495"
     CDS             complement(16062..16952)
                     /gene="glxR"
                     /locus_tag="VKU03_14495"
                     /EC_number="1.1.1.60"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008679 - 2-hydroxy-3-oxopropionate
                     reductase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0046487 - glyoxylate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxy-3-oxopropionate reductase"
                     /protein_id="HLJ72525.1"
                     /translation="MTDIGFIGLGVMGRPMAANLIAAGHKLFLRSGRSVPGDLIKAGG
                     VACATNKEVAQKAEIVITMVPDTPQVEEVLFGENGVAAGLSKGKIVVDMSSISPIATK
                     EFAKKVNAIGADYLDAPVSGGEVGAKAASLTIMVGGPEAAFNRVKPIFEKLGKNITLV
                     GGNGDGQTTKVANQIIVALNIEAVAEALVFAAKAGADPAKVRQALMGGFASSRILEVH
                     GERMVKRTFDPGFRIELHQKDLNLALQGAKALGVSLPNTASAQELFNVCAAHGGKAWD
                     HSAMVRALEIMADHEVAKKS"
     gene            complement(17076..17861)
                     /gene="hyi"
                     /locus_tag="VKU03_14500"
     CDS             complement(17076..17861)
                     /gene="hyi"
                     /locus_tag="VKU03_14500"
                     /EC_number="5.3.1.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318004.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxypyruvate isomerase"
                     /protein_id="HLJ72526.1"
                     /translation="MPKFDANLTFLFNELPFLDRFGAARKAGFPGVEYMSPYEATKQE
                     IAARLKDNGLTQVLFNLPVGDWAKGDRGIAIFSDRTDEFKRGVAKALDYADALGCKQI
                     NCLAGLAPKDADQNALRKALVGNLSYAAAELGKAGVRLLIEPCNTRDMPGFFLNRSGQ
                     GFDIIKEVGSDNLFLQYDIYHMQIMEGDLARTIEANLPRIAHLQLADNPGRNEPGTGE
                     INYPFLFGYLDKIGYHGWIGCEYRPRTTTVEGLGWLKAMNAAA"
     gene            complement(18048..19853)
                     /gene="gcl"
                     /locus_tag="VKU03_14505"
     CDS             complement(18048..19853)
                     /gene="gcl"
                     /locus_tag="VKU03_14505"
                     /EC_number="4.1.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009028 - tartronate-semialdehyde synthase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glyoxylate carboligase"
                     /protein_id="HLJ72527.1"
                     /translation="MARMKAVEAAVRVLEKEGVTCAFGVPGAAINPLYAALKARGSIR
                     HILARHVEGASHMADGYSRAKAGNIGVCIGTSGPAGTDMITGLYAAAADSIPILCITG
                     QAPRAKLAKEDFQAVDIESIAKPVTKWAVTVREPALVPMVFAQAFHVMRSGRPGPVLI
                     DLPIDVQTAEIEFDDSTYQPLPAYKPAATRQQAERALAMLNAADQPLIVAGGGIINAD
                     ASDLLVEFAELTGVPVIPTLMGWGIIPDDHPLMAGMVGLQTSHRYGNATYLESDFVFG
                     IGNRWANRHTGSIEVYTKGRKFVHVDIEPTQIGRVFGPDFGIVSDAKAALKLFIDVAR
                     EMKKAGKLKDRSGWAAKCLHRKNNMLRKSHFDEVPLKPQRVYQEMNEAFGRDTCYVTT
                     IGLSQIAGAQFLKVYQPRNWINCGQAGPLGWTLPAALGVRAADPEKTIVALSGDYDFQ
                     FMIEELAVGAQHKLPYLHMVVNNSYLGLIRQSQRGFQMDFEVSLAFDNINAAPEGDAV
                     PGYGVDHVAVAEGLGCKAIRVKSPNEFKDAFAKARALMEEHQVPVVVEFILERVTNIS
                     MGTEVDNINEFEEILCLDPSLSLKKSQDLRMPEKV"
BASE COUNT         3280 a         6634 c         6630 g         3666 t
ORIGIN      
        1 agtcgtgaac aggcgcagga agcggccgac atcggcatcc tccgtgttgc gccagaactg
       61 ccagtaatcg tatggcgaca gcaggtcggc gtcgagccag accgcgccgg cggccgtctt
      121 gcccattttc gcgcccgagg ccagcgtgat gagcggcgag gtcagcgcat aaagctgggc
      181 ggttcccagc cggcggccga gatcgacgcc ggtgacgatg ttgccccact ggtcggagcc
      241 gcccatctgc aggacgcagc catagcgtcg atgaagctcc acaaaatcat aagcctgcag
      301 gcacatataa ttgaattcta agaaagaaag ctcctggtcg cggctcaggc gcaacttcac
      361 cgagtccatc gccagcatgc gattgacgga aaagtggcgg ccgacttcgc gtaaaaattc
      421 aatgtagttg agcttggcca gccattccgc gttatccggc atgacggcgt ctgtcttgct
      481 ttcgccaaat gtcataaacc gcgagaaagc cttacgaatc ccagccttgt tggcctcgat
      541 ctgctggacg gtcagaagct tccggctctc gtcccggccg gagggatcgc cgaccatcgt
      601 cgtgccgccg cccatcaggg cgagcggctt gccgccgcat ttctgcagcc aatggagcag
      661 catgatctgg accagcgagc cgacatggag cgacggcgcc gtgcaatcga agccgatata
      721 ggcgatcagc gagccgtcgg tcgccttggc gtcgagcccg gctggatcgg agcattgatg
      781 gacgaagccg cgctcataga gcgtcgccag gaaatcggat cgcgggcgga acgtctcggc
      841 caagcctttg ctcctaaaaa acgcgccgag cattagggac ttgagcagcg ttatgcaata
      901 agccgggcgt tgcgtccggt tctcgcccgc catgcccgcc cctttgagcg aagacgatct
      961 gatcgcccgt ttctttgcgc cgatggccgc gccggaaggc ctcggcctga aggacgacgc
     1021 ggcgctcctc gccggccggc ctggcgatct cgtcctgacc aaggacgccc tggtcggcgg
     1081 cgtgcatttc ttcgccgacg atccgcccgg cgcgatcgcc cgcaaggcgc tgcgggtcaa
     1141 tctctccgac ctcgccgcca aaggcgccga gccggtcggc tttctgctgg cgctcgcttt
     1201 gccgggcggc tggaccgagg attggctcgc cgccttcgcc gctgggttag ccgacgacgc
     1261 cgcaacctat cgctgcccgc tctttggcgg cgacaccgtc aagacgcccg ggccgctcat
     1321 ggtttcgatc accgccttcg gccggatcga ggcgggacgg atggcgcggc gcgatggggt
     1381 caagccgggc gaccgcctct atgtcagcgg aacgatcggc gacgccgctc tcggtctggc
     1441 gctgagactc ggcgcgccgg cgcttgccgg cctcgaagcg gcaagccgcg cctttctgct
     1501 cgaccgctat ctcctgccgc ggccgcgcac cgctctggcc tcggcaatgc gacaattcgc
     1561 gcatggcggc atggacgttt cggacggatt tgtcggcgat ctgaccaaga tgctccgcgt
     1621 cagcggcgtg tccgggcgga tcgatctctc gcacctgccg ctttcgcccg ccgctgaggc
     1681 cgcgctcgcc gctgagaacg ggcttttgga gacgatcgca acgggcggcg acgattacga
     1741 aatcctcgcc gcgatcgagc cggcggaagc gccggctttt gagcgcgccg cggaagaagc
     1801 cggggtcaga atcacacgcg tcggcgaagc tgtcgcgggg aagggcgcgc cgcaattcac
     1861 cgcgcccgga agccgcgcca tcgttttcca gcgcggctcg ttcagccatt tctaaggagt
     1921 tgatgacgtg aggctcgacg cgaccgccct gcaaaaccgc cttctcgccg cttgcgcggt
     1981 cgcgcgcgaa gcgggcgcgc tcgcccggcg gcgctttctc gatcggacgt ccttcacggt
     2041 cggcttcaag gggccgcagg atttcatcac cgaagtcgac ggcgaggtcg aaaagctgat
     2101 ccgcgcccgc ctgatcggca ttttcgaaca agacggtttc atcggcgaag aaggcggcgc
     2161 cgcgccgggc gccgaaggcg cgccgtcctg ggtggtggat ccgatcgacg gcaccgccaa
     2221 tttcgcccgc ggcgcgcctt tctactgcgt ctccatcgcc ttgatccgca atcgcgaaat
     2281 tgaagtcggc gtcatctacg atccgatgct ggacgagctc ttcgccgccc ggcgcggcgc
     2341 aggcgcggtt gtgaacggcg tcccgctcaa ggtttccgaa acggcttcgc tcgccagggc
     2401 gacggtcgag gtcggctgga acatgcggtt cggcgctgag gattttctcg cggtgatggg
     2461 ccggatcgtc gcgaccggcg ccggcgtcgt ccgcgccggc tcgggcgcgc tcggcctggc
     2521 ctatatcgcg gccgggcggc gggacggcta tgtcgagacc catatcaaag cctgggactg
     2581 cctggccggc gtgttgctcg tccgcgaggc cggcggctat gccagcgatt tcctggccgg
     2641 cgagggcctc agccaaggca atccgctgat cgcctgcgcg cccggcgtca aggaggcgct
     2701 gatcgaggcg gcggcgatca aggacctggc gctatgaccg agcgctcttc ggcgcgcgag
     2761 gcccacgcgg cgggacgagg tcccgccaat ttcgattggc gctgagcgcg ggaatgggct
     2821 agctgccaat cttcgcgacg gaggggacat gacagatctg gtgcggctga gcgcgggcga
     2881 ggcgagcctt gcgatcgcgc cggaaggcgc ggaggcgcgc gaatggcgga tcgccgggcg
     2941 cgatctgcta tggagcggcg atccggcctt ttggaatcag atcagtccga tcctgtttcc
     3001 cgtcgttggc tggacccggg acggcgcccg tgtcgacggg cgcttctatc cgctccggct
     3061 gcacggcttc gccgccgcgc agctttttgc gctcgacaag caagagccgg cgaaggcgcg
     3121 cttcgtcctg acggacaacg atgagacgcg agccgtctac ccgttcgcct tccgcttggc
     3181 cgtcgaatac agcctgacgc cgacgcggct ggaaatcgcg cttgaaatgg agaatcgcgg
     3241 ttcgggcccg gcgccttacg cgttcggcct tcatcccgga tttcgctggc ctttcgacgg
     3301 cgccggccgc gacggctgcc gcgtcgtgtt cgaaaagcct gagagcggcg acattccggt
     3361 tcttgcgccg ggcggcatgg tctcgccgcg ccgacggccg attccgatga aaggaaacgt
     3421 tctcgatctg aacgacgagc ttttcgccaa ggacgcgctc tgcttccttt cgcccgcgag
     3481 ccgatggctg cgctttgtcg gcgccagcgg cgcggcgatc gaaatggcgc tgggcgattt
     3541 tccgcacgcc gcgctgtgga tgcggccggg cgcgcccttc ctgtgcatgg aggcctggac
     3601 cggctatagc gatcctgaag gtttcgccgg cgatctcttt gaaaagccgg ccatgcgggt
     3661 cgtcgcgccg ggcgagaagc agcgttgcgt cgccgcctat acgtaccttc cgccgagcgc
     3721 cgccggagcg tcctcatgaa tcaagccgtg acctctatcc tgaagccctc ggccgagcgc
     3781 gcgctgatca tcgacggcaa ggcggcgtcg caggccctgc tggcgcgcct cgccgaagag
     3841 gcgcggcgct tgatcgccgc cggcgtcagg cccggcctct ccgtggtcct ggtcggcgag
     3901 gaccccgcca gccaggtcta tgtccgctcg aaagggagag tcgcaagcga gctcggcttt
     3961 cgctccgagc agcacgatct ttccccggcg accagcgagg aggacctgct cgctctggtc
     4021 gagcgcctca acgccgatcc aggcgtgcac ggcgtcctgg tgcaattgcc gctgcccaag
     4081 cacatccgct cggcccgcat cctcgaagcg atcgcgccgc ataaggatgt cgacggcttc
     4141 catccgatca atgtcggctt ggcggcgatc ggcgaggcca agcgcgcgct cattccctgc
     4201 acgcccgccg gcgcgatgat cctgatcgag aaggcctgcg ccgcgctcgg cgagagcctc
     4261 gccggcaagg aggcggtggt gatcggccgg tcgaacattg tcggcaagcc gctcgcccgc
     4321 ttgctgctcg ccgccaattg caccgtgacc gtcgcccatt cgcgcacgcg cgacctcgcg
     4381 gccgtggcgc gccgcgccga tctcctcatc gccgctgtcg gccagccgga actcgtgcgc
     4441 ggcgactggg tgaagcccgg cgcgatcgtg atcgatgtcg gcgtcaaccg cattccgggc
     4501 gaggggctca acgcctcggg ccagcccaag accaggctcg tcggcgatgt cgcctttgcg
     4561 gaagccgcgc agcgggcggc ggcgatcacg ccggcgccgg gcggcgtcgg cccgatgacc
     4621 atcgccatgc tgatggccaa cacgctgatc gccgcctgcc gcgcgcaggg cttggaagag
     4681 ccgaagcttt gacgccgagc cgcgccagaa tttcgtcgaa aaacctttcc gcccgcgaat
     4741 agatcaggcg gccgaattat cggacctcgt tgagcggagc gtcgaccggt aagtattgcg
     4801 cgtgagggag aggaattccc gtcggatttg cgctggcgcg ttgcaggcgc tcttgctttc
     4861 tggcaagagg gcgcccggaa ttcttgtcga tcgctggctc aggggcttga acaagccgaa
     4921 aggcgcggcg gaaaggcctt aaggactgaa cgaatgacca ttgtgctgat tctgatcatt
     4981 ctgagcgggt tgctgtcggt cgcctatggc gggtttacga tcagccagct gatggcggcc
     5041 gacgccggca ccaagcgcat gcaggaaatt tccggcgcgg tccgggaggg ggcgcaagcc
     5101 tatttgcggc ggcaatatac gaccatcgcc ggggtcggcg tcatcgtctt tatcggcctc
     5161 gctctgctcc tctcgattga tgtcgcgatc ggcttcctga tcggcgcggt gctctccggc
     5221 gccgccggct tcatcggcat gaacgtctcg gtgcgcgcca atgtacggac cgcgcaagcg
     5281 gcgacgaaat cgctcggcgc cggcctcgag atcgctttca agtcgggcgc cgtcaccggc
     5341 atgctggtcg ccggcttggc cctgctcggg gtcgctttct attactggat tttgaccgga
     5401 ccgctgggct tgacgccggc cagccgcacg gtgatcgacg cgcttgtcgc gcttggcttc
     5461 ggcgcttcgc tgatctcgat cttcgcccgt ctcggcggcg gcatcttcac caagggcgcc
     5521 gatgtcggcg ccgaccttgt cggcaaggtc gaggccggca ttccggagga tgacccgcgc
     5581 aatcccgcca cgatcgccga caatgtcggc gacaatgtcg gcgattgcgc cggcatggcg
     5641 gccgatcttt tcgagaccta cgcggtgacc gttgtcgcca ccatggttct cgcctcgatc
     5701 ttcttcgccg gcggcgcttc gctcgtgccg gccatgctct atcccctggc gatttgcggc
     5761 tgttgcatcg tgacctcgat catcggcgcg caattcgtcc agctcggcgc caacaattcg
     5821 atcatgggcg cgctctataa gggcctcatc gtcaccggcg tcctgtcgat cctcggtctg
     5881 gccgtggcga cgcaattcgt catcggctgg ggcgacatcg gaacggtcca cgacaaggcg
     5941 gtgacgggcg ccaacctgtt catctgcgga attgtcggcc tcatcgtcac cgggctgatc
     6001 gtcgtcatca ccgaatatta taccggcacc ggcaaacggc cggtcgtgtc gatcgcgcaa
     6061 gcctcggtga cgggacacgg caccaacgtg atccaaggcc tggcggtgtc gctcgaagcg
     6121 accgccttgc cggcgctggt gatcattggc ggcatcatcg cgtcgtttca acttgccggc
     6181 cttttcggca tcgcagtggc ggtgacgacg atgctgggcc ttgccggcat gatcgtcgcg
     6241 ctcgacgctt tcggaccggt caccgacaat gccggcggca tcgccgaaat ggccggcctg
     6301 ccgaaagagg ttcgccaatc caccgacgcg ctcgacgcgg tcggcaacac gaccaaggcg
     6361 gtgaccaagg gctatgcgat cggctcggcg ggccttggcg ccctcgtcct cttcgccgcc
     6421 tattcgaacg atctgaccta tttcgcctcg aatcccgaca agttccccta tttcaaggat
     6481 atcggccagg tctcgttcga actgtcgaat ccatatgtcg tcgccggcct gattttcggc
     6541 ggcctcattc cgtacctttt cggcggcatc gccatgaccg cggtcggccg cgcggcgggt
     6601 tcggtggtgt tggaagtgcg ccgccagttc cgcgagaagc cggggatcat gcaagggacg
     6661 gacaggcccg actatggccg agccgtcgat atgctgacca aggcggccat ccgcgagatg
     6721 atcattccct cgcttctgcc ggtcctggcg cctttcgtcg tctatttcgg cgttctgttc
     6781 atttctggcg agaagaagga ggccttcgcc gcgctcggcg cctcgctgct cggcgtcatc
     6841 gtcaacgggc tcttcgtcgc cgtctcgatg acctccggcg gcggcgcctg ggacaacgcc
     6901 aagaagagtt tcgaggacgg gttcaccgac aaggacggcg tgaagcacct caagggctct
     6961 gacgcgcata aggcgtcggt gaccggcgat acggtcggcg acccttacaa ggacaccgcc
     7021 ggcccggccg tgaacccggc gatcaagatc accaacatcg tcgcgctgct gctgctggcg
     7081 atcctggcgc attgaggcgg aaggtatgta tgggaaggaa gggcggggag gacccgccct
     7141 ttttcgttga ggggctaagg atcgatgtat ctttaaaccc aaccggttcg cgcagttctg
     7201 gttggacctg caatccgcct tcgatatccg tatcgcggaa cgcgaagccg gcgccgaaat
     7261 caaatggctc ccgacgcgat cgatctctca aaggcggaac agacggcgcg agccggcgcg
     7321 cgccgtcccg atttcagcaa agcgagctca atcgatgcgc ctcgtccaga tccgccgcta
     7381 tcccgtcaaa ggcctgcgcg gttattcgtt gcaaagggcg gaggtgcagc gcatcggcct
     7441 cgccggcgat cggcgatgga tgattgttga cgaagcgggc aagttcgtca cgcagcgcca
     7501 gtgtccgaag ctcgcgcaga tcgatgcttc tcctaccgcg cgcggcgtcg ccctgcgcca
     7561 tgccacgcat ggcgcgatcg agatcgatgt tcccgacgcg cgcgcgccga tcgagacggt
     7621 cgtcgtctgg cgcgacgcgg ttcaggcgcg ggtcgccgaa tccgccggcg cctacctttc
     7681 ggctttcctg gagaagcccg tccggctggt ctttctgcac gacgcgagcg agcgccgcgt
     7741 cgacccggct ttcggccgcg ccgaggatcg cgtcagcttc gccgacggct ttccgatgct
     7801 gatcacgtcg gccgcctcgc tcgccgacct caacggccgc ctgccgcaag cgatcggcat
     7861 ggaccgcttc cgcccgagcc tcgttatcga cggcgcgccc gcctgggcgg aggatacgtg
     7921 gcgcaaggtg cggatcggcg ggctctcgtt ccgcatcgcc aagccctgct cgcgctgcgc
     7981 cgttccgacg ctcgatcctt taaccggcga agcgctcgat ggaaacgagc cgatccgcac
     8041 gctcggcgcc tttcaccgcg ccgccaatgg cgggatcatt ttcggccaga acgcgattcc
     8101 cgacgatgtc ggcgcgctgc aggtcggcga tccagtcgaa atcgtcgaaa ccggctcctc
     8161 gaacctgctc taaggtagac gatcgtcatt gcgagcgaag cgaagcaatc cactcgtagg
     8221 tacagactta tgagcggact gcgtcgtcgc tgcgctcctc gcaacggcgg cgcccttctt
     8281 acgagaaccg gaacaaattg ttgaacatgt tgcgcaggaa gctcggctcc tggccgccgg
     8341 tcggcgtcgt gcgcgagacg aagtcgaaaa tctcgccgtc cttcaagcca taattggcga
     8401 tgcgctcgac cttgccggtc ttcggcgcga aatagatggc gaggacgttc tggtcctcca
     8461 tcttgaccgg catgaaagcc agcgcccgat gcatcttctg gccgatatag taccaggcgt
     8521 cgccgccgac cgtggaggtg gtcgaaggcg ttcccatcag gctgagcact tcctgcttgg
     8581 tcgtcgcgcc gattttgacc tgggaatagg tcttctcgtc gatctggtag ccccggtcga
     8641 aatcgccgtc atagccgagg cagccgcaaa ggctggtcgc gagcgacagg acgcacagaa
     8701 cgcgcgcggc gccggtccgc aaggcccctg aactccccat caaagcttct ccgattcccc
     8761 gcgctttttg cttgcgcttg cgcgccgcga tgcctaaccc aaggcgctcc tcaaatgcaa
     8821 ggcggcggcg acatggtcct tgcgagatgg cgcgcccggc gcgccaaccg cgatctgatc
     8881 gagcaacttc acggcgagat cgtggcggcg gcgcgccggc cggcgcttta tgccgatttt
     8941 cacgcccccg acacgctgga tggacggttc gagatgatcg cattgcatgc cggccttatc
     9001 ttgcgccggc tccacgccat atccggcctg ggcgacgaaa tcgcgcagga tttggcggat
     9061 tgcctctttc gccactttga tgtcgccttg cgcgagatcg gcgtcggcga cacttcgatc
     9121 cccaagcgga tgaagcggat ggccgaagct ttctacggcc ggaacaaggc ctatgcggag
     9181 gggttggcgg cgcccggaaa cgaccgcctg gcggaggcgc tcgcgcgcaa cgtctatggc
     9241 gcggcgagcc tcgccgaggc cccgtacgcg ccggctctgg cgcgctttgc gcgcgattgc
     9301 gcggccgcgc tcgatcgcct cggcctcgaa gatttcgcgg ccggccgcat cgcctttccg
     9361 gagccctcgg cggcccagcc atgacgccgc ccttcagcag gcctgttcgg gtcgaggcca
     9421 tcccgcgcga cgggctggaa acgcgcatcg acgcggatgc cggcgagcgc gcggccctgg
     9481 ccgccttcaa tgggctgccg gcggtcgccg cgctcggcgc ggcgttcagc ctcaagcatg
     9541 gcggcggcgg aacgatcctg gtccgcggcc aactcaccgc cgaagtcacc cagacctgcg
     9601 tcgtgaccct ggagcccttc gacgcctcca tttccgcgcc gatcgacctc cgcttcgcgc
     9661 cggcgggcga ggcttccgcg gggcctgact cagccgcgag cgaagccgag gcgatgctgg
     9721 tcgtcgggga ggaggacgag cccgatccga tcgtcgacgg cgtgatcgat ctcggcgcgg
     9781 tcgcgtcgga atttctcacg ctcaatctcg atccctatcc gcgcaagccc ggcgccgcgt
     9841 tcgaagcgcc agcctcgggc ggcgacggcg cttccgactc gccgttcagc gtgctcgccg
     9901 agcgcaagaa ggacgcctga tccggagcgc ggcgtttctt tcgcgaggcc ggcgcgaggg
     9961 gccgtttggt cagggcattc cattgaaatc cgtcgcgaaa cgggttgcgc cgaaacggct
    10021 gtgaaccctt tgcggccgac atttgcgttg cgcgccgaag cggttattgt ccgcgccgcc
    10081 ggggggcggg ggcgtttccg agaggcgcgg tcgggtggtc gctggcatga acaaggacgt
    10141 ccgcatagcg ctggacgcta tgggcggcga ttacggaccg aaagttgtcg cgccggccgc
    10201 ctcgatcgct ttaacccgcc ggccggatgt cagctatctg tttttcggcg acgagaagct
    10261 tatccgcccg cttgtcgagg ccgacgccaa tttgaagcgc aagacgcgaa tcattcacac
    10321 cgatgtcgcc gtgaagatga gcgacaagcc gagccaggcc ctgcgcgccg gccggcgctc
    10381 ctcctcgatg tggctggcgc tcgacgcggt caagaaaggc gaggctgatg tcgcggtttc
    10441 cgccggcaat accggcgcgc tgatggcgat gtcgaaaatc tgcctcagga ccatgccgca
    10501 gatcgatcgt ccggccatcg ccgcgatgtg gccgaccttg cgcggcgaat cgattgtgct
    10561 cgatgtcggc gcgacgatcg gcgccgacgc gcagcatttg atcgaccttg cggtcatggg
    10621 ctccgccatg gcccggatcg tcttcgatat cgagcggccg accgtcggcc tgctcaatgt
    10681 cggcgtcgag gagatcaagg gcctcgaaga ggtcaaggcg gccgggcggg cgctgcgcga
    10741 ggcgaatttc cccaatctcg attatcgcgg cttcgttgag ggcgacgatc tcggcaaagg
    10801 cacggtcgac gtgatcgtca ccgagggctt taccggaaat atcgcgctga agaccgccga
    10861 gggcacggcg cggcagatcg cgcaatatat ccgtgacgcg atgcgccaca gcttcttcgc
    10921 ccggatcggc tacgttttcg cgcgccgcgc tttcgaggcg ctgcgcgaca agctcgatcc
    10981 cagcaaggtg aatggcggcg tctttctcgg tcttgacggc gtcgtcatca agagccatgg
    11041 cggcgccgac gccgagggct atgccggggc gatcgaagtt ggctacgaca tggtccgcca
    11101 cgaactgctc ggcaagacgc gcgatatgat cgcccaggcc aacgaatcgc gcgcccaacc
    11161 tccgcccatg cttgcggcgg agtcttgatc gcccaaccag gataagattt tgacggcgcc
    11221 tctgaaaacg cttcgctcgg tcgtgcgcgg aaccggctcc tacctgccgg cgcggatcat
    11281 gagcaacgcc gagctctcga ccatggtcga gaccagcgac gaatggatcc agcagcgcag
    11341 cggcatccgc gagcgccata tcgcggccga aggcgaaacc acctcgattc tggcgacgcg
    11401 cgccgccgaa gccgcgctcg ccaacgccgg cctgaccggc gccgacatcg acctcgtgat
    11461 cgtcgcgacg tcgacgccgg attatacgtt ccccgccgcc gccacccagg tgcaggccaa
    11521 tctcggcatg cgtcacggcg tcgctttcga cctgcaagcg gtctgttccg gcttcgtctt
    11581 cgccgtcgcc accgccgaca agttcctgag ctcgggctcg catcagcgcg ctttggtgat
    11641 cggcgccgag accttctcgc gcattctcga ttggaaggac cggacgacct gcgttctgtt
    11701 cggcgacggc gccggcgcga tcgtgctgga ggcgcaggaa agcgacggcc ggctcggctc
    11761 gccgggcgtc atcaccgcgc aattgcgctc ggacggcgcg catcgcgcca agctctatgt
    11821 cgatggcggc ccgtcctcga cccagacggt cggccatttg cggatggagg gccgcgaagt
    11881 gttccgccac gcggtcggca tggtcaccga cgtgatcaag gcggccttcg ccgccgccga
    11941 tgtgaccgcc gccgatatcg actggttcgt gccgcatcag gccaatcgcc ggatcatcga
    12001 cgcttcggcc gaaaagcttg gcatcgcgcc ggaaaaggtg gtcaagactg tcgaccagca
    12061 cggcaacacc tcggccgcct cgatcccgct ggcgctcgac atcgcctgcc gcgacggccg
    12121 catcaagaag ggcgacctgg tcatgatcga ggcgatgggc ggcggcttca cttggggcgc
    12181 ggccttgatc cgctggtaaa agcgtccgcc agcaaatgat tatgcgacgg aaccgaaatt
    12241 ttctctgacc cgccaatatc ttcccgattg cacgactctc gccgcggtga ttcttttgaa
    12301 ggcgtctggg gacgccgaca aagatgggaa tcgccgattt tccacagagc acgcggcggg
    12361 tcgaatggcc gaccctcggc ctcgcgatcc tgatctacgg cttgtggttc ggcgcgacct
    12421 ttttccatga ccgcctgccg ctctggctgc tgacgccggt cgcggcctgg accgtcgcct
    12481 ggcatatgag cctgcagcac gaaatcatcc atggccatcc gacgcgcctg cgctggctca
    12541 actcgctgat cggcgcctgg cccttggcgc tgtggctgcc cttcgaggac tatcgccaca
    12601 cgcatctgac ccatcatcgc gacgagcggc tgaccgatcc gctcgacgat ccggaatcct
    12661 tctactggcg tcaggaggat tgggacgcgc ttgggccaat agggcgcgcg ctcgtgcgct
    12721 tgcaggcgac gctgctcggc cggatggtca tcggtcccgc ctggtcttgc gccaaactgt
    12781 ggcagcgcga gatccagcgc ctggcgcggg gcgaaaagcg ggcgctccgc gacgtcgctt
    12841 tgcacctcgc cgaaatggcg gccgtcctcg cctgggtcgt cggcgtctgc aaaatgccga
    12901 tcgtcgaata cgtcctttgc ttcgcctatg ccggcacgtc gctggccttg gtgcgctcct
    12961 tcgccgagca tcgcgccgaa agcctggtcg agcggcgcac cgcgatcgtc gagcgctcat
    13021 ggctgctcgg gccgctcttc ttgttcaaca atcttcatct cgcccatcac atgcgcccgg
    13081 cgatgccttg gtacaagctg ccgggctggt acgccgccaa tcgcgcggcc ctgatcgagc
    13141 gcaatggcgg gctgctttat gtgacctatt tcgacgtgct gcgccgctat ctgctgcgcc
    13201 cgcacgacca gacgctgcat cccttgcagg gcgaggacgg cgcgagcagg gcggcgtagg
    13261 aactccgcca ttgcgagggg cgcagcgacg aaatccattc attgagcgcc gcgtcctatg
    13321 aatggattgc ttcgcgtcgc tcgcaatgac ggcgcgccgc cgtgacgatg acggcgcgag
    13381 gctggcgcgc tatagcgaag cgccgcgcgg cggccctccc tcgattcgga gcggattatc
    13441 gatggatgcg cttgtcgcct ccttgggcat gtatgacttc ccctttctcg ccgaggccaa
    13501 tcggcgccta tgggcggcga tcggcggctc gcttcgcgcc gccggaatcg acgcgccggt
    13561 cgcgctcgat cgctctgagc catgcgaagt ttgggccaat ccgcgcctcg tcttcggcca
    13621 aacttgcggc tatcctttcg tcacggcgtt gcgcgatcgc gtgagcctgc tggcgacgcc
    13681 gatctattct ttcgaaggct gcgaaggccc gaaccattgc agcttcgtca tcgcccgcgc
    13741 cgggagccgc ggccgcagcc tcgccggctt ccagggggcg agagccgccc tcaacgcgcg
    13801 cgacagcaat accggcatga acctgtttcg cgcgctgatc gcgccgctcg cgcgcggccg
    13861 gccgatgttc agccaggcgc tcgtcaccgg ctcgcatgcg gcgagcctcg aagcggtcgc
    13921 ggaaggggtc gccgacatcg ccgcgatcga ttgcgtcacc tatggcctgc tgcgccgcgg
    13981 ccggccggac cttgccgaga aagtcgtcgt catcgcccgc agccaagcct cgccgggcct
    14041 gccattcgtc atcagcgcgg ccctcggcga gccgctggcg gccgcggtcc gcgtcgcgct
    14101 ctatgcggcc ctgaacgatc caggtctcgc cgaggcgcgc gaggccctcg gcctgaaagg
    14161 cgcggccatt ctggacgaga cggcttaccg gaagatcgcc gagatcgaac gcggcgccgt
    14221 cgcgctcggc tatccggaat tggcctagcg cttctcggag aggcggcccc gccgcggccg
    14281 caacgtcccc gctttccccg ctctttgcgg attggggacc agccgatgga acaaagcggc
    14341 ccgcaaggct ttgtcaggcg atcgagacgg aggctttcat gcccacacta tctctcttga
    14401 ggatcacggc gacggcggtc gcgctgacgg tctcggccgc agccttcgcg caggcgaacg
    14461 aggaaggcaa cgcgccggag aacaacgcgg cggcgacggc cccaaaaatg catatgacgc
    14521 atcgtatgcg ccatcatcat cactaccggc gctatacacg ccataggtcg agcggcagcc
    14581 agcggatggg caacgggtcg aacggaacca acccgaataa cggcgtcagc ggcgccacgt
    14641 cgggcgaggg gaacgcgcag acgacgaatc cgcaaagcaa tcagtagcgc gcctctggag
    14701 gaggttcggc gttcggcccc gaggcgggcg gcgtcaagcc gcaggttctt cctttaccgc
    14761 caagatcgcc ctgaagtcgt tgacattggt ccgcgtcggc ccggtgatca gcagatcgcc
    14821 gagcgcggaa aaggcggtgt aggcgtcatt gtcggcgagc atcgccttca gatccaggcc
    14881 gaggcgggcg gcgcgcgcca atgtatccgg gccgatgatc gcgccggcat tgtcttcgct
    14941 gccgtcgatc ccgtccgtat cggcggcgag ggccgagatt ccgagatggc cgtcgagcgc
    15001 gacggcgaga ctcagcagga attcggcgtt gcggccgccg cggcctttgc cacgcaccgt
    15061 caccgtcgtc tcgccgccgg aaatgaccgc gcaaggcggc gcggcgggtt ggccgtaatc
    15121 ggcgcattgc cgcgcgatcc cggcatgaac aatgccgcat tcgcgcgcct cgccctcgat
    15181 cgcattgccg agaatgaccg gcgcgaaacc ggcgtcgcgc gcgatcttcg cggcggcttc
    15241 gagggaggat tgcggcgtcg cgatcagcag gttcgtcaca ttggcgaggc ggagatcgcc
    15301 cggcttcggc gtctcgttgg ccggatcgga aagatgctgt cggaccgccg gcgaaacctc
    15361 gatcttgtac ttttcgagaa cccgtcgcgc gtcggcgagc gtcgtcgcat cggcgacggt
    15421 cgggccggaa gcgatcaccg agagatcgtc gccgggcacg tcggaaatga tcaggctgac
    15481 gacccgcgcc ggcgccgcag cggcggcgag ccttccgcct ttgatcgccg acagatgctt
    15541 gcgcacgcag ttcatctcgc cgatatgagc gccgctcttc agcagcgcgc ggttgatctg
    15601 ctgcttgtcg gcgagcgtga ttgcgccgcc gggcagggcg agcagggccg agccgccgcc
    15661 ggaaatcaac gccaagacga gatcgtccgc ggtcaggccg ctgaccagat cgaggatggc
    15721 ccgcgccgcg gccggcccgg cttcgtccgg caccggatgg ctcgcctcga cgactttgat
    15781 gcgcgtcgtc ggcgcgccat ggccgtagcg ggtcacgacc aggccttcga gcgggccgcg
    15841 ccaatgcgcc tcgaccgcgg cggccatcgc cgccgccgcc ttgcccgcgc cgacgacgat
    15901 cgtgcggcct ttcggcggcg gggggagatg cgacggcacg caatgcgccg gcgccgcggc
    15961 cgccaccgcg gcgtcgaaca tgcgtttgag cagggcgcga tgatcggcgg tcatgtcgta
    16021 atctgcgccc gttcctgtga gagcgtgtcg accggttcag ctcagctttt cttcgccacc
    16081 tcgtggtcgg ccatgatttc cagcgcgcgc accatcgccg aatgatccca cgccttgccg
    16141 ccatgggcgg cgcaaacatt gaacaattcc tgcgccgacg cggtgttggg caaggagacg
    16201 cccagcgctt tcgcgccttg cagcgcgaga ttcaggtctt tctggtgaag ctcgatgcgg
    16261 aagccgggat cgaacgtccg cttcaccatg cgctcgccat gcacttcgag aatgcgcgag
    16321 gaggcgaagc cgcccatcag cgcctggcgg accttggccg gatcggcgcc ggccttggcg
    16381 gcgaagacca aggcctcggc gaccgcctcg atgttcaacg cgacgatgat ctgattggcg
    16441 accttggtcg tctggccgtc gccattgccg ccgaccaggg tgatgttctt gccgagcttc
    16501 tcgaaaatcg gcttgacccg gttgaacgcc gcctcgggcc cgccgaccat gatggtgagc
    16561 gaagcggctt tcgccccgac ttcgccgccc gagaccggcg cgtcgagata atcggcgccg
    16621 atcgcgttca ccttcttggc gaattccttg gtcgcgatcg gcgagatcga gctcatatcg
    16681 acgacgatct tgcccttgct caatcccgcg gcgacgccgt tctcgccgaa caggacctct
    16741 tcgacctgcg gcgtatcggg caccatcgtg atgacgatct ccgctttctg cgcgacctct
    16801 ttattggtcg cgcaggcgac gccgcccgcc ttgatcaggt cgcccggcac ggagcggccg
    16861 gagcggagga aaagcttatg cccggccgcg atgagattgg cggccatggg acggcccatc
    16921 acgccaaggc cgatgaaacc gatgtcggtc attgctgaag agactcctta agttcatttt
    16981 tctccgtcat ttgcgaggag cgaagcgacg aagccatcct tcgtaaggcg acgactcatg
    17041 aatgggttgc ttcgtttcgc tcgcaatgac gggcctcacg ccgccgcatt catcgccttg
    17101 agccagccga gcccctcgac cgttgtcgtg cgcggccggt attcgcagcc gatccagcca
    17161 tgatagccga tcttgtcgag atagccgaac aggaacggat agttgatctc cccggtgccc
    17221 ggctcgttgc ggcctggatt gtcggcgagc tgaagatggg cgatgcgcgg caggttggct
    17281 tcgatggtgc gggcgagatc gccctccatg atctgcatgt gatagatatc gtactgcagg
    17341 aagagattgt cggagccgac ctctttgatg atgtcgaatc cctggccgga gcggttcagg
    17401 aaaaagccgg gcatgtcgcg cgtgttgcag ggctcgatca gcaggcgaac gcccgccttg
    17461 ccaagctccg ccgccgcgta tgaaaggttg ccgacgagcg ctttgcgcag cgcgttttga
    17521 tcggcgtctt tcggcgcgag gccggccagg caattgatct gcttgcagcc gagcgcgtcg
    17581 gcatagtcga gcgccttggc gacgccgcgc ttgaattcgt ccgtgcggtc ggaaaagatc
    17641 gcaatgccgc gatcgccctt ggcccagtcg ccgaccggca ggttgaaaag gacctgcgtc
    17701 aagccattgt ctttgagccg cgccgcgatc tcctgcttcg tcgcctcata gggcgacata
    17761 tattcaacgc cggggaatcc ggcctttcgc gccgccccga agcggtcgag gaacggcaat
    17821 tcattgaaca ggaaagtgag attggcgtcg aatttcggca ttggtttttc cttgggaatc
    17881 ccctcgcccc gcggagcggg gagagggtgg cgcgcagcgc cgggtgaggg gctgcgcggt
    17941 ggttggcgat tagcctattt cagcgagcat gtactttcga gtcctgcgcc gcccctcatc
    18001 cggcccttcg ggccgccttc tccccgcaag cggggagaag gggagtgcta gaccttttcc
    18061 ggcatgcgca aatcctggct cttcttcagc gacagcgacg gatcgaggca gagaatttcc
    18121 tcgaattcgt tgatgttgtc gacctccgtt cccatcgaaa tattggtgac gcgttcgagg
    18181 atgaattcga ccacgaccgg cacctgatgc tcctccatca aggcgcgcgc tttggcgaaa
    18241 gcgtccttga actcattcgg gcttttgacc cggatcgcct tgcagcccaa accttcggcg
    18301 accgcgacgt gatcgacgcc atagcccggc accgcgtcgc cttccggcgc ggcgttgata
    18361 ttgtcgaaag cgaggctgac ttcgaaatcc atctggaagc cgcgctgcga ctgccggatt
    18421 aatccaaggt acgaattgtt cacgaccata tggagatagg gcagcttgtg ctgtgcgccg
    18481 accgccagct cctcgatcat gaattgaaaa tcgtagtcgc cggagagcgc gacgatggtc
    18541 ttttccggat cggcggcgcg cacgccgagc gcggcgggca gcgtccagcc gagcgggccc
    18601 gcctggccgc aattgatcca gttgcgcggc tgatagactt tcaggaattg cgcgccggcg
    18661 atttgcgaca ggccgatcgt ggtgacgtag caagtatcgc gcccgaacgc ctcgttcatc
    18721 tcctggtaga cgcgctgcgg cttcagcggc acctcgtcga aatggctctt gcgcagcatg
    18781 ttgttcttgc gatgcaggca cttggcggcc cagccgctgc ggtccttgag cttgccggct
    18841 ttcttcatct cgcgcgcgac gtcgatgaag agcttaagcg cagccttggc gtcggagacg
    18901 atgccgaaat caggcccgaa cacccggccg atctgggtcg gctcgatgtc gacatggacg
    18961 aatttgcggc cctttgtata gacctcgatc gagccggtat ggcggttggc ccagcggttg
    19021 ccaatgccga agacgaaatc ggattcgagg tacgtcgcgt tgccgtagcg gtggctggtc
    19081 tgcaggccga ccatgccggc catgagcggg tggtcgtcgg gaatgattcc ccagcccatc
    19141 agggtcggga tcaccggcac gccggtcagc tcggcgaatt cgacgagcag gtcggaagcg
    19201 tcggcgttga tgattccgcc gccggcgacg atcaagggtt ggtccgccgc gttgagcatg
    19261 gcgagcgccc gctcggcctg ctggcgggtc gccgccggct tgtaggccgg caggggctgg
    19321 taggtggaat cgtcgaattc gatctcggcg gtctgtacgt cgatcggcag atcgatgagg
    19381 accgggcccg gacgtcccga ccgcatgaca tgaaaagctt gcgcgaagac catcggcacg
    19441 agcgccggct cgcgcaccgt caccgcccat ttggtcactg gcttggcgat cgattcgatg
    19501 tcgaccgcct gaaaatcctc cttggccagc ttggcgcgcg gcgcctggcc ggtgatgcag
    19561 aggatcggga tcgaatccgc cgccgcggcg taaaggccgg tgatcatgtc cgtgccggcc
    19621 gggcccgacg tgccgatgca gacgccgata ttgcccgcct tggcgcggct atagccgtcg
    19681 gccatatgcg aggcgccctc gacatgccgg gcgaggatgt gccggatcga gccgcgcgct
    19741 ttcaaagccg cgtaaagggg attgatcgcc gcgccgggca cgccgaaggc gcaggtcacg
    19801 ccttctttct ccagcacgcg gacagcggct tcgactgctt tcattctcgc catggcggcc
    19861 tcctggtccg gcgggcttgc gcccaagatc gcgaagccga ttttcgtgga cgcgaaggcc
    19921 ttcagcaatg gcgggccatt ttcttttccg tcagatggaa agactgcgat ttattttgca
    19981 atatcagggg gatacgagaa tgaaaatcta gaaccttcag gccttggccc gcccggcggc
    20041 atattcgagc ctgagctcga aggaatgtag aaatacgcta aacgcgaaag ccttcgagcg
    20101 gagggaagag gctacgcggc gcgcggccgg acatcccgtt cgatcgcgcc cccgctttcc
    20161 tgtttcgctt tggtgagatc gtgcatcgcc tggagattga tccagaattc
//
LOCUS       DATGJW010000483         4470 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_93076, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000483 DATGJW010000000
VERSION     DATGJW010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4470)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4470)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4470
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93076"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(144..1562)
                     /locus_tag="VKU03_14510"
     CDS             complement(144..1562)
                     /locus_tag="VKU03_14510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662132.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase subunit beta"
                     /protein_id="HLJ72528.1"
                     /translation="MGKVTGFLEIDRADRRYAPASDRIRHYREFVIPLSEESNRNQAA
                     RCMNCGIPYCHNGCPVNNQIPDWNDLVYRGDWAEAARNLHTTNNFPEITGRICPAPCE
                     ASCTLNLEDTPVTIKTIECAIADRAFAEGWIKPDPPAAKSGKKIAVVGSGPAGLACAQ
                     QLARVGHDVHVYEKNAKPGGLLRYGIPDFKLEKRHVERRVQQMKAEGVTFHCNTMIGR
                     DLSAQELVEKYDAVALAGGAEAPRDLKAQGRELSGVHFAMDFLPQQNRRVSGEPLGTN
                     EPITASGKHVVVIGGGDTGSDCIGTSIRQGAVKVTQLEILPKPPDKEDKLLTWPDWPL
                     KLRTSSSHEEGAERDFAVMTSAIEGENGAVTGLRCIRVDAKMQPIAGTEFLLKADLVL
                     LAMGFVSPVRDGLLADLGVALDNRGNVQASTKNYETSVNKVFACGDMRRGQSLVVWAI
                     REGRQCAQAIDKALMGTSVLPR"
     gene            complement(1614..>4470)
                     /gene="gltB"
                     /locus_tag="VKU03_14515"
     CDS             complement(1614..>4470)
                     /gene="gltB"
                     /locus_tag="VKU03_14515"
                     /EC_number="1.4.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glutamate synthase large subunit"
                     /protein_id="HLJ72529.1"
                     /translation="TYPVEKGAAGMEHALNRLCERAEAAVHGGYNIIILSDRQVGPDR
                     IPIPALLATAAVHHHLIRKGLRTSVGLVVETGEAREVHHFCCLAGYGAEAINPYLAFE
                     TLAAMARDLPEEISEYEIIKRFIKSVDKGILKVMSKMGISTYQSYCGAQIFDAVGLSS
                     VFVDRFFFGTATRIEGASLLEIAEETALRHADAFGASPVLRSALDVGGDYAFRLRGEA
                     HSWSPQTVSLLQHAVRGNAQDKYRAYAKLLNEQDEKLLTIRALFRIRGAAEDGRAPVP
                     LDEVEPAKDIVKRFSTGAMSFGSISREAHTTLAIAMNRIGGKSNTGEGGEETDRFKRL
                     ANGDSMRSAIKQVASGRFGVTAEYLVNSDMMQIKMAQGAKPGEGGQLPGHKVDAVIAK
                     VRHSTQGVGLISPPPHHDIYSIEDLKQLVFDLKNVNPEAQVSVKLVSEVGVGTVAAGV
                     AKCKADHVTISGYEGGTGASPLTSIKHAGSPWEIGLAETHQTLVLNRLRSRISVQVDG
                     GLRTGRDVVVGALLGADEFGFATAPLIAAGCIMMRKCHLNTCPVGVATQDPVLRKRFI
                     GQPEHVINFFFFVAEEVREWMAAMGYRRFDEMVGQMQMLDRAQAIEHWKAKGLDFTKL
                     FHKPKAGPGVGIYRSEPQDHGIDDVLDRQFLAEAREAIEHGRHVRLEKPIRNSDRSTG
                     AMLSGVIARRYGHAGLRDEAIHIKLKGTAGQSFGAWLAHGVTLELEGDANDYVGKGLS
                     GGRIVVYPPREATKLAPEQSIIVGNTVLYGAIAGECYFRGVAGERFAVRNSGAIAVVE
                     GAGDHGCEYMTGGVVVVIGETGRNFAAGMSGGIAYVLDEAGDFAERCNLSMVELEPVA
                     DEEELMQRLHHHGGDLEGHGRVDVMADMSGRDAERLHQLIVNHARYTGSARARRILEN
                     WDAYLPKFRKVMPVEYRRALAELAAQRGREPFLAAGE"
BASE COUNT          717 a         1472 c         1470 g          811 t
ORIGIN      
        1 agcccgctgc gcagagcttg gtcggttcgt ccaacggtcg gtcgggctat ggccgcttga
       61 cgatgccggg ccgatgtggc cccgcgtcga gatgcgtgca tgccgtagcg cttcgcgggg
      121 aaaggggatt ttctcgcgcc gcgctacctc ggcagcacgc tcgttcccat cagcgccttg
      181 tcgatcgcct gcgcgcattg gcggccttcg cggatcgccc agacgaccag cgattggccg
      241 cgccgcatgt cgccgcaggc gaagaccttg ttgacgctcg tctcatagtt tttcgtgctc
      301 gcctgcacgt tgccgcgatt gtcgagggcg acgccgagat cggcgagcaa gccgtcgcgc
      361 accggcgaga cgaagcccat cgccaacagg acgaggtcgg ccttcagcag gaactccgtc
      421 ccggcgatcg gctgcatctt ggcgtcgacg cggatgcagc gcagcccggt cacggcgccg
      481 ttctcgcctt cgatcgcgct ggtcatcacc gcgaaatcgc gctcggcgcc ttcttcatgg
      541 ctcgacgagg tgcgcagctt caacggccag tccggccagg tcaggagctt gtcctccttg
      601 tccggcggct tgggcaggat ttcgagctgc gtcaccttga ccgcgccctg gcggatcgag
      661 gtgccgatac agtccgagcc ggtgtcgccg ccgccgatca ccacgacatg cttgccgctg
      721 gcggtgatcg gctcgttggt gccgagcggc tcgccggaga cgcgccggtt ctgctgcggc
      781 aggaagtcca tcgcgaaatg cacgccggaa agctcacggc cctgcgcttt cagatcgcgc
      841 ggcgcttcgg cgccgccggc cagcgccacg gcgtcgtact tctccaccag ctcttgcgcg
      901 gaaagatcgc ggccgatcat cgtgttgcaa tgaaaggtca cgccctcggc cttcatctgc
      961 tggacgcggc gctcgacatg gcgcttttcg agcttgaagt ccggaatgcc gtagcgcagc
     1021 aagcccccgg gcttggcgtt cttctcataa acatgaacgt cgtgcccgac gcgcgcgagc
     1081 tgctgggcgc aggcgagccc ggccggcccc gagccgacca ccgcgatttt cttgccgctc
     1141 ttggccgccg gcgggtccgg cttgatccag ccctcggcga aggcgcggtc ggcgatcgcg
     1201 cattcgatcg tcttgatcgt gaccggcgta tcttcgaggt tcagcgtaca tgaggcttcg
     1261 caaggcgccg gacaaatgcg gccggtaatc tccggaaagt tgttggtcgt gtgcaggttg
     1321 cgcgccgcct ccgcccaatc gccgcgatag acgaggtcgt tccagtcggg gatctggttg
     1381 ttcaccgggc atccgttgtg gcaataggga atgccgcaat tcatgcagcg cgccgcctga
     1441 ttgcggttgc tctcctcaga cagcgggatg acgaattcgc gatagtggcg gatgcggtcg
     1501 gaggccggcg catagcgccg gtccgcgcgg tcgatctcca gaaaacccgt caccttgccc
     1561 attgctgtcg cccgccctgc ctctcgtgca aatgcttctt acgttccatc gttctattcg
     1621 ccggcggcga ggaacggctc gcggccgcgc tgcgccgcaa gctcggccaa ggcgcgccga
     1681 tattcgaccg gcatcacttt gcggaatttc ggcagatagg cgtcccagtt ctcgaggatg
     1741 cgtcgcgccc gcgccgagcc ggtgtagcgg gcgtgattga cgatgagctg gtgcagccgt
     1801 tcggcgtcgc gcccgctcat atcggccatc acatcgaccc ggccatggcc ctcgagatcg
     1861 ccgccgtgat gatggagacg ctgcatcagc tcctcctcgt cggcgaccgg ctccaattcg
     1921 accatcgaca gattgcagcg ctcggcgaaa tcgccggcct cgtccagcac ataggcgatg
     1981 ccgcccgaca tgccggcggc gaagttgcgc ccggtctcgc cgatcacgac gacgacgccg
     2041 ccagtcatat attcgcagcc gtgatcgccc gcgccctcga cgacggcgat cgcgcccgaa
     2101 ttgcgcacgg cgaagcgctc gccggcgacg ccgcggaaat agcattcgcc ggcgatcgcg
     2161 ccatagagca cggtgttgcc gacgatgatc gactgctccg gcgcgagctt cgtcgcttcg
     2221 cgcggcggat agacgacgat gcggccgccc gacaggccct tgccgacata gtcgttggcg
     2281 tcgccttcga gctccagggt gacgccgtga gcgagccagg cgccgaagct ctggccggct
     2341 gtgcccttga gcttgatatg gatcgcctcg tcgcgcagcc cggcatggcc gtagcgccgc
     2401 gcgatgacgc ccgacagcat cgcgccggtc gagcggtcgg aattgcggat cggcttttcg
     2461 agccgcacat gccggccatg ctcgatcgcc tcgcgcgcct cggcgaggaa ttgccggtcg
     2521 aggacgtcat cgatgccgtg atcctgcggc tcgctgcgat aaattccgac gccggggccg
     2581 gccttgggct tgtggaagag cttcgtgaaa tcgagaccct tggccttcca atgttcgatc
     2641 gcctgcgcgc gatccagcat ctgcatctgg ccgaccatct cgtcgaaacg gcgatagccc
     2701 atcgccgcca tccattcgcg cacttcctcg gcgacgaaga agaagaagtt gatgacatgc
     2761 tcgggctggc cgatgaagcg cttgcgcagg accgggtctt gcgtggcgac gccgaccggg
     2821 caggtgttga gatggcactt gcgcatcatg atgcagccgg cggcgatcag cggcgcggtg
     2881 gcgaagccga attcatcagc gccgagcagc gcgccgacca cgacgtcgcg cccggtgcgc
     2941 aatccgccgt cgacctgcac ggaaatgcgc gagcgcaagc ggttgagcac caaggtctga
     3001 tgcgtctcgg cgaggccgat ctcccagggg ctgccggcat gcttgatcga ggtcaaaggc
     3061 gaagcgccgg tgccgccttc gtagcctgaa atcgtcacat gatcggcctt gcacttggcg
     3121 acgcccgccg ccaccgtgcc gacgccgact tccgagacga gcttgaccga gacctgagcc
     3181 tcaggattga cgttcttcaa gtcgaagaca agctgcttca aatcctcgat cgaatagatg
     3241 tcgtgatgcg gcggcggcga gatcaggccg acgccttgcg tcgaatggcg caccttggcg
     3301 atgacggcgt cgaccttgtg gcctggcaat tggccgccct cgccgggctt ggctccttgc
     3361 gccatcttga tctgcatcat gtcggaattg acgagatatt ccgcggtcac gccgaagcgg
     3421 ccggacgcga cctgcttgat cgccgagcgc atcgaatcgc cattggccag ccgcttgaag
     3481 cggtccgtct cctcgccgcc ctcgccggta ttcgacttgc cgccgatccg gttcatggcg
     3541 atggcgagcg tcgtatgagc ttcgcgcgag atcgagccga aggacatcgc gccggtcgaa
     3601 aagcgcttga cgatgtcctt ggccggctcg acctcatcga gcgggaccgg cgcgcggccg
     3661 tcctccgcgg cgccgcggat gcggaacaag gcgcggatgg tcagaagctt ttcgtcctgc
     3721 tcgttgagca gcttggcgta ggcgcggtac ttatcctgcg cattgccgcg caccgcatgc
     3781 tgcagcagcg agacggtttg cggcgaccag gaatgagctt cgccgcgcag gcgaaacgcg
     3841 tagtcgccgc cgacatcgag cgcgctgcgc aagaccggcg aggcgccgaa cgcgtcggca
     3901 tgccgcaagg cggtctcctc ggcgatttcc agcaggctcg cgccttcgat gcgcgtcgcg
     3961 gtgccgaaga agaagcggtc gacgaaaacg ctggaaaggc cgacggcgtc gaaaatctgc
     4021 gcgccgcaat aggattggta cgtcgatatg cccatcttgg acatcacttt caggatgccc
     4081 ttgtcgaccg acttgatgaa gcgcttgatg atctcgtatt cgctgatctc ctcgggcagg
     4141 tcgcgcgcca tcgcggccag cgtctcgaag gcgaggtacg gattgatcgc ctcggcgccg
     4201 tagccggcga ggcagcagaa atggtggact tcgcgcgctt cgccggtctc gacgacgagg
     4261 ccgaccgagg tcctcaagcc cttgcggatc aggtggtgat ggactgccgc cgtcgccagc
     4321 agcgccggga tcgggatgcg gtccggcccg acttggcggt cggagaggat gatgatgttg
     4381 tagcctccat gcaccgccgc ctcggcgcgc tcgcaaaggc ggttcagcgc atgctccatg
     4441 ccggccgcgc ctttctcgac cggataggtg
//
LOCUS       DATGJW010000484         2133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_196707, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000484 DATGJW010000000
VERSION     DATGJW010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2133
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_196707"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..725)
                     /locus_tag="VKU03_14520"
     CDS             complement(<1..725)
                     /locus_tag="VKU03_14520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009028640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72530.1"
                     /translation="MLDLGAPAPALALRGLRKSFAGKPALFDLDLDVRAGEFYALLGP
                     NGAGKTTTLRIVAGLLQPDQGEIAVFGVDARRRPIDAKRIIAWLPDEPMLYDKLDPLE
                     YLEFVAGLWGVEPRRAQKLAEELLETLGLWEHRRERCEGFSRGMKQKVALAGALIHEP
                     KLLMLDEPLTGLDAAVARQVKDLLVARVKAGATIILTTHILEVAERLADRIGIIQRGR
                     LIAEGTLDELRRAAGARLGGASL"
     gene            complement(770..1675)
                     /gene="rsmA"
                     /locus_tag="VKU03_14525"
     CDS             complement(770..1675)
                     /gene="rsmA"
                     /locus_tag="VKU03_14525"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261034.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HLJ72531.1"
                     /translation="MSLAQDGLPPLREVVAAYDLSPKKALGQNFLFDLNLTGRIARAA
                     GPLEGVAVVEIGPGPGGLTRALLAHGAKKVIAVERDARCLAALQAVGAHWPGRLDLIE
                     ADALSLDDNVLIEKAAGAPIRICANLPYNIATVLLARWLEAEPWPPFYERLTLMFQRE
                     VAQRIVATPAERADYGRLAVLAGWRTEARILFDVPAAAFTPPPKVVSSVVELVPRPQP
                     AACDPRLLGAATQAAFGQRRKMLRQSLKGFAASLRTEAGVLLAAAGIDPTRRAEEVGV
                     DGFVALARIAARFAQPVNGAASCGP"
     gene            complement(1672..>2133)
                     /locus_tag="VKU03_14530"
     CDS             complement(1672..>2133)
                     /locus_tag="VKU03_14530"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019401501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLJ72532.1"
                     /translation="ALTCEAIIKTGRIVARDLQTRFKIARPRLAIAGLNPHAGEGGAI
                     GREEIDVIAPAIVALRGEGHDVVGPLSADTMFHAAARKKYDAAIVMYHDQGLIPLKAL
                     AFDEGINVTLGLPFIRTSPDHGTAFDIAGQGRANPSSLIAAARLAARLAAS"
BASE COUNT          353 a          700 c          759 g          321 t
ORIGIN      
        1 tcgaggctgg cgccgcccag gcgcgccccg gccgcccggc gcaattcgtc gagcgttcct
       61 tccgcgatca gccgtccgcg ctggatgatg ccgatgcggt cggcgaggcg ctcggcgact
      121 tcgagaatat gcgtcgtcaa gatgatcgtc gcgccggctt tgacgcgagc gacgagcagg
      181 tccttgacct gccgcgccac agcggcgtcg agcccggtca gcggctcgtc gagcatcaga
      241 agcttcggct catggatcaa cgcgccggcc aacgcgactt tctgcttcat gccgcgcgag
      301 aagccctcgc agcgctcgcg ccggtgctcc cagaggccga gcgtctccaa tagctcttca
      361 gcgagctttt gggcgcggcg cggctcgacg ccccagaggc cggcgacgaa ttcgagatat
      421 tcgagcggat cgagcttgtc gtagagcatc ggctcgtcgg gcagccaggc gatgatcctc
      481 ttggcgtcga tcggccggcg ccgcgcatcg acgccgaaaa ccgcgatttc gccctgatcc
      541 ggctgcagca ggccggcgac gatgcgcaag gtcgtcgtct tgccggcgcc gttcgggccg
      601 agaagcgcgt agaattcgcc ggcgcgcacg tcgagatcga ggtcgaaaag cgccggcttg
      661 ccggcgaagc tcttgcgcaa gcctcgcagc gcgagagccg gcgcgggcgc accgagatcg
      721 agcatggaaa gtctcatcga gccgacaaca gccggcggcc gaggcagttt taggggccgc
      781 agcttgcagc cccgttaacg ggctgggcga aacgcgccgc gatccgcgcc aaagcgacaa
      841 agccgtcgac gccgacctcc tcggcgcgcc gggtcggatc gatccccgcc gcggcgagca
      901 gaacgcccgc ttcggtccgc agggacgcgg cgaagccttt cagggattgg cgcagcattt
      961 tgcgcctttg gccgaaagcc gcctgggtcg cggcgccgag caggcgcgga tcgcaggccg
     1021 ccggttgggg ccgcgggacc agctcgacga cgctggagac gactttcggc ggcggcgtga
     1081 aggccgcggc gggcacgtcg aataaaatgc gcgcttccgt ccgccagccg gcgagcaccg
     1141 cgagacggcc gtaatcggcg cgctcggccg gcgtcgcgac gatgcgctgg gcgacctcgc
     1201 gctggaacat cagagtcaag cgctcataga agggcggcca tggctcggct tcgagccagc
     1261 gcgcgagcaa aaccgtcgcg atattgtatg ggagattcgc gcagatgcgg atcggcgcgc
     1321 ccgccgcctt ttcgatgaga acattatcgt cgaggctcag cgcgtcggct tcgatcaggt
     1381 cgagccgccc cggccaatgc gcgccgaccg cctgcaaggc ggcaaggcag cgggcgtcgc
     1441 gctcgacggc gatgactttt ttcgcgccgt gagcgagcaa agcgcgcgtc aggccgcccg
     1501 gcccgggccc gatctcgacg acggcgacgc cctcgagcgg gccggcggcg cgggcgatgc
     1561 ggccggtgag gttaagatcg aacaggaaat tctggccgag cgctttcttc ggcgacagat
     1621 cgtaagccgc cacgacttcg cgcagcggcg gcagcccgtc ctgggcgagg ctcatgaagc
     1681 ggcgagccgc gccgccaggc gcgccgcggc gatcaggctc gaaggattgg cgcggccctg
     1741 gccggcaatg tcgaacgccg tgccgtgatc gggcgaggtg cggatgaagg gcagcccgag
     1801 cgtcacgttg atcccttcgt cgaaagcgag cgcctttaaa gggataagac cctgatcatg
     1861 gtacatcacg atggcggcgt cgtatttctt gcgcgccgcg gcgtggaaca tcgtatcggc
     1921 ggataaggga ccgacgacgt catgtccttc gccgcgcaag gcgacgatcg ccggggcgat
     1981 gacgtcgatc tcctcacggc cgatcgcgcc gccttcgcca gcatgcggat tgagcccggc
     2041 gatcgcgaga cgcgggcgag cgattttgaa gcgcgtttgc aggtcgcggg cgacgatacg
     2101 gcctgttttg atgatcgctt cgcaggtcag cgc
//
LOCUS       DATGJW010000485         3546 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1230947, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000485 DATGJW010000000
VERSION     DATGJW010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3546)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3546)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3546
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1230947"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(63..650)
                     /gene="hisB"
                     /locus_tag="VKU03_14535"
     CDS             complement(63..650)
                     /gene="hisB"
                     /locus_tag="VKU03_14535"
                     /EC_number="4.2.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403599.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004424 - imidazoleglycerol-phosphate
                     dehydratase activity [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazoleglycerol-phosphate dehydratase HisB"
                     /protein_id="HLJ72533.1"
                     /translation="MRSANVTRKTKETDISVELLLDGAGKADIQTGIGFFDHMLDQVA
                     RHSLIDLTIRAKGDLHIDQHHTVEDVGIAVGQALTKALGDKRGIARYADCLLPMDEAL
                     TRVALDLSGRPFLVFKAEFPSGKIGVFDTELAREFFQALAVHAQMTLHIENLYGANSH
                     HIAESCFKGVARALGAAIAIDPRQAERIPSTKGAL"
     gene            801..1373
                     /gene="hslV"
                     /locus_tag="VKU03_14540"
     CDS             801..1373
                     /gene="hslV"
                     /locus_tag="VKU03_14540"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011083476.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004298 - threonine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0051603 - proteolysis involved in protein
                     catabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease subunit HslV"
                     /protein_id="HLJ72534.1"
                     /translation="MSSEPAPGAQSWHATTIVMVRKGGKAVIGGDGQVSLGQTIIKGN
                     AKKVRRLAKGEVIAGFAGATADAFTLFERLEAKLEQYPGQLARACVELAKDWRTDRYL
                     RRLEAMMLVADKTTGLVLTGTGDVLEPEASEHGAAMGIGSGGNYALAAARALMTTDLD
                     AETIARRALQIAAEICVYTNDNVVIESIAT"
     gene            1370..2455
                     /locus_tag="VKU03_14545"
     CDS             1370..2455
                     /locus_tag="VKU03_14545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72535.1"
                     /translation="MISDATLAQAVRDELISAAQADGLRALEAQTAASAAPAWAAAPT
                     PDDESLRLVSGFGDVFVAIGLALFFGAAGFFVQHFFGRLAMWLAVAVLAWLLAEFFTR
                     RRRMALPSIILLVVFAGCVFIALLQILTGSDLFASNNIIGVAKGGGEDRPIWLAGVAT
                     AGAAALHYWRFRVPITIAAGAAALAAAFVAFGLSLSLQPSRGLVSALVLVCGLAIFSL
                     AMAFDGSDRERATRRTDIAFWLHLLAAPMIVHPVISTFVNDTSGVETGGAIAILVVFL
                     VFALVAVAIDRRAMLVSGLVYAGVAFGSLLRQTGLSDMNIPATLLALGVFVLLLSAGW
                     QPLRRAILRFFPSPLARRLPHPVRNPS"
     gene            2452..>3546
                     /gene="hslU"
                     /locus_tag="VKU03_14550"
     CDS             2452..>3546
                     /gene="hslU"
                     /locus_tag="VKU03_14550"
                     /EC_number="3.4.25.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018265039.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent protease ATPase subunit HslU"
                     /protein_id="HLJ72536.1"
                     /translation="MTDFSPREIVSELDRYIVGQNEAKRAVAIALRNRWRRLRLEGAM
                     REEVMPKNILMIGPTGCGKTEISRRLAKLAGAPFLKVEATKFTEVGYVGRDVEQIVRD
                     LVEIGISLVRDKKRKALQAKAQLAAEERVLDALVGANASAATRDSFRRKLRDGELDEK
                     EIEIELPSASQGLPLFELPNMPGASVGAISIGDIFGKGFGQRAKPRRMTVKDSYEPLL
                     AAEADKLLDQDQLVREAIEEVESNGIVFLDEIDKICAREGRLGGDVSREGVQRDLLPL
                     IEGTTVTTKHGSVKTDHILFIASGAFHVSRPSDLLPELQGRLPIRVELASLTEEDFKR
                     ILTETEASLIKQYVALMRTEGVELSFAPDAI"
BASE COUNT          558 a         1146 c         1167 g          675 t
ORIGIN      
        1 tatatcggtc aacacatgaa ccggttagct ctttgacgaa gccgctttac cgccgctcgc
       61 ggtcagagcg cgcccttagt ggagggaatg cgctcggcct ggcgcggatc gatcgcgatc
      121 gccgcgccca gcgcgcgcgc cacgcccttg aagcaggatt cggcgatatg gtggctgttg
      181 gcgccgtaga ggttctcgat atgcagagtc atctgggcgt ggacggcaag ggcttggaaa
      241 aactcgcgcg ccaattcggt gtcgaagacg ccgatcttgc cgctcggaaa ctcggccttg
      301 aagaccagga acggccggcc ggaaaggtcg agcgcgacgc gggtcagcgc ttcgtccatc
      361 ggcagcaggc aatcggcgta gcgcgcgatc ccgcgcttgt cgccgagcgc cttggtcagc
      421 gcctggccga ccgcgatgcc gacatcctcg accgtatggt gctggtcgat atgcagatcg
      481 cccttggcgc ggatcgtcag gtcgatcagc gagtgacgcg cgacctgatc cagcatgtgg
      541 tcgaaaaagc cgatgccggt ctggatgtcg gccttgccgg cgccgtcgag caggagctcg
      601 acggaaatgt cggtctcttt ggtcttgcgc gtcacgttgg cgctgcgcat gtcgggtctc
      661 gggtctgggg ctgtttcggg aacgcggcgc tcaaatagca agatgcgagc cggaccgcca
      721 cctttgtcgg ggcttgcgcc ggccggacga ggctcctaga tcggagtttc cttcctcata
      781 gagcgctttc aaggccgaca atgagcagcg aaccggcgcc cggcgcccag tcctggcacg
      841 ccacgaccat cgtgatggtg cgcaaaggcg gcaaggcggt gattggcggc gacgggcagg
      901 tcagcctcgg ccagacgatc atcaagggca acgccaagaa agtgcgccgc ctcgccaagg
      961 gcgaggtgat cgccggcttc gccggcgcga ccgccgacgc cttcaccctg ttcgagcggc
     1021 tggaagccaa gctggagcaa tatcccggcc aattggcgcg ggcttgcgtc gaattggcca
     1081 aggattggcg gacagaccgc tatctgcgcc ggctcgaagc gatgatgctg gtcgccgaca
     1141 agacgaccgg cctggtcctg accggcaccg gcgacgtgct ggagccggaa gcaagcgagc
     1201 atggcgcggc gatgggaatc ggctcgggcg gcaattacgc cctcgccgcg gcgcgcgcgc
     1261 tgatgacgac cgatctcgac gccgagacga tcgcgcgccg cgcgctgcag atcgccgccg
     1321 aaatctgcgt ctacacgaac gacaatgtcg taatcgaaag catcgccaca tgatttcgga
     1381 cgcgaccctg gcgcaagcgg ttcgcgatga gctgatcagt gcggcgcagg ccgacggctt
     1441 gcgcgcgctg gaggctcaaa ccgccgcttc cgccgctccc gcctgggcgg cggcgccgac
     1501 gcccgacgac gagagcttgc gcttggtctc cggcttcggg gatgttttcg tcgcgatcgg
     1561 cttggctttg ttcttcggcg cggcgggctt ttttgttcag catttcttcg ggcggctcgc
     1621 gatgtggctc gccgtcgccg ttttggcttg gctgttggcg gaattcttca cccgccgccg
     1681 gcgcatggcg ctgcctagca tcatcctgct cgtggttttc gccggctgcg tctttatcgc
     1741 cttgcttcag attttgaccg gctcggacct gttcgcctcg aacaacatta tcggcgtcgc
     1801 caagggcggc ggcgaggaca ggccgatctg gctggccggc gtcgcgacgg ccggcgccgc
     1861 cgctttgcat tattggcgct ttcgcgtgcc gatcaccatc gccgccggcg cggcggcgct
     1921 ggcggcggcc ttcgtcgctt ttgggttgtc cctttcgctt cagccgagcc gcgggcttgt
     1981 cagcgctctc gtccttgtct gcggcctcgc catcttttcg ctggccatgg cctttgacgg
     2041 ctccgatcgc gagcgcgcga cgcggcggac ggacatcgct ttctggctgc atcttttggc
     2101 cgcgccgatg atcgtccatc cggtgatttc cactttcgtc aacgatacga gcggcgtcga
     2161 gactggcggc gcgattgcga tcctcgtcgt attcctggtc tttgcgctgg tcgcggtcgc
     2221 catcgatcgc cgcgcaatgc tggtttcggg cttggtctat gccggagtcg ctttcggctc
     2281 gctgctgcgc caaaccggcc tttccgacat gaacattccc gctacgctgc tggcgcttgg
     2341 cgttttcgtc cttctgttga gcgcgggatg gcagccgctg aggcgcgcga ttctgcgttt
     2401 ctttccctcg cccttggcgc gtcgccttcc gcatccggtc cgtaatccct catgacagat
     2461 ttttcccccc gcgagatcgt ctccgaactc gaccgctaca ttgtcggcca gaacgaggcc
     2521 aagcgcgccg tcgccatcgc cttgcgcaat cgctggcgcc gcctgcgcct cgaaggcgcg
     2581 atgcgcgaag aggtgatgcc gaagaacatc ctgatgatcg gcccgaccgg ctgcggcaag
     2641 acggaaatct cgcgccgcct cgccaagctc gccggcgcgc cgttcctgaa agtcgaggcg
     2701 acgaagttca ctgaggtcgg ctatgtcggc cgcgatgtcg agcagatcgt gcgcgatctc
     2761 gtcgaaatcg gcatttctct ggtccgcgac aagaagcgca aggccctgca ggccaaggcg
     2821 caactcgccg cggaggagcg cgtgctcgat gcgctggtcg gcgccaacgc ttccgccgcg
     2881 acgcgcgatt cctttcgccg caagctgcgc gacggcgagc ttgacgagaa agagatcgag
     2941 atcgaattgc cgagcgccag ccagggcctg cccttgttcg aactgccgaa catgccgggc
     3001 gcctcggtcg gcgcgatttc gatcggcgat attttcggca agggcttcgg gcagcgcgcc
     3061 aagccgcgcc gcatgacggt gaaggattcc tacgagcctc tgctcgccgc cgaggcggac
     3121 aagctgctcg accaggacca gctcgtccgc gaggcgatcg aagaggtcga gagcaacggc
     3181 atcgtgtttc tcgacgagat cgacaagatt tgcgcgcgcg aagggcggct cggcggcgac
     3241 gtgtcgcgcg aaggcgtcca gcgcgatctg ctgccgctga tcgaaggcac gaccgtgacg
     3301 accaagcatg gctcggtcaa gaccgaccat atcctgttca tcgcctcggg cgccttccat
     3361 gtgtcgcggc cgtcggacct gctgcccgaa ttgcagggcc ggctgccgat cagagtcgag
     3421 ctcgcttcgc tcaccgagga ggatttcaag cgcatcctca ccgagaccga agcaagcctg
     3481 atcaaacagt acgtcgccct gatgcgcacc gagggcgtcg agctctcttt cgcgccggac
     3541 gcgatc
//
LOCUS       DATGJW010000486         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_922889, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000486 DATGJW010000000
VERSION     DATGJW010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_922889"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..685
                     /locus_tag="VKU03_14555"
     CDS             110..685
                     /locus_tag="VKU03_14555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894693.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005267 - potassium channel activity
                     [Evidence IEA];
                     GO_function: GO:0015252 - proton channel activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TMEM175 family protein"
                     /protein_id="HLJ72537.1"
                     /translation="MGTERLQAFTDGVIAIVITIMVLEIKAPSDATLAALLASLPILL
                     TYVFSFVNVGIFWNNHHHMLHAAERVDGKALWANLFLLFWISLVPFVIRWIDETGFQA
                     LPTAAYGAVLAMSAFGYLLLEWALVSCNGPNSALALAVGRDRKAIISMALYGVAILLA
                     FWSPWAAIVVYVALSLAWFLPDRRIETLKRE"
     gene            792..1412
                     /locus_tag="VKU03_14560"
     CDS             792..1412
                     /locus_tag="VKU03_14560"
                     /EC_number="1.1.1.298"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonic semialdehyde reductase"
                     /protein_id="HLJ72538.1"
                     /translation="MTVHAKIDHDHAPLAEAALAQIFTEARTHNAFLDKPVPDELLRR
                     AVDLAKIGPTSANNSPLRVLFLKSKAAKERLRPFLAPGNLDKTMAAPVTAITAYDLQF
                     YEHLPFLMPHADYKGYFAGLPAEARRETAHKNGTLQVAYLLIALRAVGLDTGPMTGFD
                     NEKLDAEFFPDGRFKSNVLINIGYGDHAKLFPRSPRFSFEEMAQIL"
     gene            1516..1950
                     /locus_tag="VKU03_14565"
     CDS             1516..1950
                     /locus_tag="VKU03_14565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605565.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLJ72539.1"
                     /translation="MEWSVLPRNWAPYTQSLFRIMTGLLFLEHGTAKLLSFPPGAAPP
                     ESFGMLLVTGLIELIGGALIAAGFLTRPVAFVLSGYMAAAYFIAHAPRGLFPILNHGE
                     LAILYCFAFLYFAAAGAGAWAVDAWLARRAPKEQSHDAMYRA"
     gene            complement(2112..>2625)
                     /locus_tag="VKU03_14570"
     CDS             complement(2112..>2625)
                     /locus_tag="VKU03_14570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72540.1"
                     /translation="HKNDAITALLSRKLPSVITRGLGGALPKNALALADPETPQTPER
                     AALLAKAAALSAPSPLRPSPPALTPLERAARLYAPLPSLGPFKPALVAVKIGKSDFGV
                     MIAPKTPSAALGGTPPAAPASPHADALAVAFMPTAQIPDASGGKPLAHLRADVFAEPA
                     SEAGMLRGTD"
BASE COUNT          430 a          855 c          839 g          501 t
ORIGIN      
        1 agataagcgt cttatttgcc ggttgcaacg aaggctcggt tattgcgagc tcctcgcaaa
       61 gacggctgag aatttgcttt gcatagtttg ccatagatcg ggaggcggta tgggaaccga
      121 gcggctgcag gcgttcaccg acggcgtgat cgccatcgtc atcacgatca tggttctgga
      181 gatcaaggcg ccgtccgatg caacgctcgc cgcgctattg gccagcctgc cgatcctgct
      241 cacctatgtc ttcagcttcg tgaatgtcgg catcttctgg aacaaccatc accacatgct
      301 gcacgccgcc gagcgggtcg acggcaaggc gctgtgggcg aatttgttcc ttctcttctg
      361 gatatccctg gtgccgttcg tcatccgctg gattgacgag accggcttcc aggctttgcc
      421 gaccgccgcc tatggcgcgg ttctggcgat gtcggcgttc ggctatctgc tgttggaatg
      481 ggcgctcgtc tcgtgcaatg ggccgaattc ggccctagcc ttggcggtgg gccgcgaccg
      541 caaggcgatc atatcaatgg cgctttacgg ggtcgccatc ctgctcgcct tctggtcgcc
      601 atgggccgcg atcgtcgtct acgtggccct cagcctggcc tggttcctgc cggatcggcg
      661 catcgagacg ctgaaacgcg aatgaccgcg ctggcggtta tcacctgaac cgatagacac
      721 cagcagattt tcctcggttg aatcgatgcc gcggcgcccc attttgcgca ttgcaacagg
      781 ggagtccgcc aatgaccgtc catgccaaga tcgaccatga ccatgcgccg ctcgccgagg
      841 ctgcgctcgc gcaaatcttc accgaggcgc gcacgcataa cgccttcctc gacaagcccg
      901 tcccggacga gttgctgcgc cgggcggtcg atctcgccaa gatcgggccg accagcgcga
      961 acaattcgcc gctgcgcgtc ctctttctca agtcgaaggc ggccaaggag cggctgcggc
     1021 cttttctcgc gccgggcaat ctcgacaaga ccatggcggc cccggtgacc gcgatcacag
     1081 cctacgacct gcaattctac gagcatctgc cgttcctgat gccccacgcc gactacaagg
     1141 gctatttcgc cgggctgccg gcggaggcgc gccgcgaaac ggcgcataag aacggcacgc
     1201 tgcaagtcgc ttatctattg atcgcgctgc gcgctgtcgg actcgacacc ggcccgatga
     1261 ccggcttcga caatgaaaag ctcgacgccg aattctttcc cgacggccgc ttcaaatcga
     1321 acgtgctgat caatatcggc tatggcgatc acgcgaagtt gttcccgcgc agcccgcggt
     1381 tttcattcga agaaatggcg cagattctat aacggcgccg ttccgccgaa gcttcgcctc
     1441 attcggtttg ttcccggtgg gggcgaggcg ttcgcggctt gattcaggcc ggacaagcta
     1501 aagaggaatc gcggtatgga atggtcggtg ctgccccgca attgggcgcc ctatacgcag
     1561 tcgctgtttc gcatcatgac cggcctgctc tttctcgagc atggaacagc gaagctgctg
     1621 agctttccgc ccggcgcggc gccgccggaa tctttcggca tgctgctcgt caccggcctc
     1681 atcgaattga tcggcggcgc gctgatcgcg gccggcttct tgacccggcc ggtcgctttc
     1741 gttctttcgg gctatatggc cgccgcctac ttcatcgccc atgcgccgag gggcctcttt
     1801 ccgatcctca atcacggcga attggcgatc ctctactgct tcgccttcct ctatttcgcc
     1861 gcggccggcg ccggcgcctg ggcggtggac gcttggctcg ctcgccgcgc ccccaaggaa
     1921 cagagccacg acgcgatgta ccgagcgtag gccgcgcacg tatttcgcag gcgctaagga
     1981 tgtcggcgcg gtcgcggacg cgccactccc ggggcgaaaa ccgggcgggc gtttcgcgcc
     2041 tgtcgcggcg cggtcagcga ccgcggctac aagggccgta gcgcccggga attgcgtagg
     2101 cctttccggc ctcaatccgt cccgcgcaac atcccggctt cgctcgccgg ctcggcgaag
     2161 acgtccgcgc gcaaatgcgc gagcggcttg ccgccggaag cgtcgggaat ctgcgccgtc
     2221 ggcatgaaag caacggcgag cgcatcggcg tgcggcgacg ccggcgcggc cggcggggtt
     2281 ccgcccaagg cggcgctcgg cgtcttcggc gcgatcatca cgccgaaatc ggacttgccg
     2341 atcttgaccg ccaccaaagc cggcttgaac gggccgagcg acggcagcgg cgcatagaga
     2401 cgggcggccc gttcgagcgg cgtcaaagcg ggcggcgagg ggcgaagcgg cgacggcgcc
     2461 gacaaggcgg cggctttggc gagcaaagcg gcgcgctcag gcgtttgcgg cgtttccgga
     2521 tcggccaacg cgagagcgtt cttgggcagc gcgccgccga ggccgcgcgt gatgacggag
     2581 ggaagcttcc ggctgagcag cgccgtgatc gcgtcgttct tatgc
//
LOCUS       DATGJW010000487         2870 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_923063, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000487 DATGJW010000000
VERSION     DATGJW010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2870)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2870)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2870
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_923063"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1233
                     /gene="uxaC"
                     /locus_tag="VKU03_14575"
     CDS             <1..1233
                     /gene="uxaC"
                     /locus_tag="VKU03_14575"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004692190.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HLJ72541.1"
                     /translation="KPDHYVFRMLYSQGVRLEELGVQRRDGGATETDGRKIWRLFARH
                     WRLFRGTPTRLWFEHSLERVFAIRERLNEANADRLYDAIAQKLSTPDFRPRALYKSFN
                     IEAIATTDNPLDDLAAHDQIRASNWDGRVLPTYRPDFVVDPDRAGFRDNVEKLIGLAG
                     GGPATYANYLAAHHARRAYFKARGATATDHGHPTCRTADLSAAEAAALFDRVMTAGAT
                     PEEKELFRAQMLTEMARMSLDDGLAMQIHPGSFRNHNPAIFRDFGPDMGADIPTPTDY
                     VRALKPLLDKYGGDRRLTLILFTLDETAYGRELAPLAGHYPALRLGPPWWFFDSPEGM
                     RRFRELTTETAGFANTVGFNDDTRAYLSIPARHDMARRCDCAFLARLVADHRLDEDEA
                     HDVAHDLAYSLAKRAYRL"
     gene            1257..2765
                     /locus_tag="VKU03_14580"
     CDS             1257..2765
                     /locus_tag="VKU03_14580"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019997652.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mannitol dehydrogenase family protein"
                     /protein_id="HLJ72542.1"
                     /translation="MVPPRSDVSAPRLSDATLGRLPNAISRPGYDRNALQAGIVHLGL
                     GAFHRAHQAAYTEAVLNAGDRRWATIGVSLRSPETREALAPQDNLYTLALRDGAGERL
                     QIIGGLAACLVAPEDPRKLVEAMARPETRIVSLTVTEKGYCHNPATGELDESHPHIRH
                     DLAERSRPRTAIGFIVEALALRRERRLAPFTLLSCDNLPTNGETLKRVLTGYAALRDR
                     ELARFVADAVACPSTMVDRIVPATTDADRDSIAAASGLRDAWPVVAEPFAQWVIEDRF
                     PSGRPEWDRAGAMFVKEVAAFEMMKLRLLNGAHSTLAYLGYLAGCETIAETMAAPGFA
                     RLAEDFMAVEAAPTLPPLPGLDLAAYRRDLIARFCNPALRHRTWQIAMDGSQKLPQRL
                     LNTIRARLGANAPFPRLALGVAAWMRYATGVDEKGGAIDVRDPLAARIKEMTRGVSEA
                     SALVSAYFRLAEVFGADLPADARFRSAVTTALAALLTKGSARVVAGFEASAA"
BASE COUNT          457 a         1020 c          890 g          503 t
ORIGIN      
        1 aaacccgacc attacgtgtt tcggatgctc tattcgcaag gcgtgcgcct cgaagagctg
       61 ggcgttcagc ggcgggatgg cggcgcgacc gagacggacg ggcgcaaaat ctggcgactc
      121 ttcgcccgcc attggcggct cttccgcggc acgccgacgc ggctgtggtt cgagcattcg
      181 cttgagcgcg tcttcgccat tcgtgagcgc ctcaatgagg cgaatgccga tcgcctctac
      241 gacgcgatcg cccagaagct gtccacaccg gatttccggc cgcgcgcgct ctataaaagc
      301 ttcaacatcg aggcgatcgc gacgaccgac aatccgctcg acgatcttgc cgcgcatgac
      361 caaatccgcg cgtcgaattg ggacggccgc gtcctgccga cctaccggcc ggatttcgtc
      421 gtcgatcccg atcgcgccgg ctttcgcgac aatgtcgaaa agctgatcgg cctcgccggc
      481 ggcgggccgg ccacctacgc caattatctc gccgcccacc acgcccgccg cgcctatttc
      541 aaggcgcgcg gcgcgacggc gaccgatcat ggccatccga cttgccgcac cgccgatctc
      601 tccgccgctg aggccgctgc gctcttcgat cgagtcatga ccgccggcgc gacgccggag
      661 gagaaggagc tgttccgcgc ccagatgctg acggaaatgg cgcggatgag cctcgatgac
      721 gggctcgcga tgcagatcca tcccggctcg ttccgcaatc acaacccggc gattttccgc
      781 gatttcggcc ccgatatggg cgccgacatt ccgacgccga ccgattatgt ccgcgccttg
      841 aagccgctgc tcgataaata tggcggcgac aggcgcctga cgctgatcct gttcaccctg
      901 gacgagacgg cttatggtcg cgagcttgcg ccgcttgccg gccactatcc ggctttgcgc
      961 ctcgggccgc cctggtggtt cttcgacagt cccgaaggca tgcgccgctt tcgcgagctg
     1021 accaccgaga cggccggctt cgccaatacg gtcggcttca acgacgacac gcgcgcctat
     1081 ctctcgattc cggcgcggca cgatatggcg cgccgctgcg attgcgcctt cctcgcccgc
     1141 cttgtcgccg atcaccgcct cgacgaggac gaagcgcatg acgtcgcgca cgaccttgcc
     1201 tatagcttgg cgaaacgagc ctatcgcctg taagcatgat tggcctctga cccgctatgg
     1261 tcccgccgcg ctccgacgtc tccgctcccc gtctttcaga cgcgacgctc ggccgcctgc
     1321 cgaacgcgat cagtcggccg ggctacgacc gcaacgcgct gcaagccggc atcgtccatc
     1381 ttgggctcgg cgcctttcat cgcgcgcatc aggcggccta tacggaagcc gttctgaacg
     1441 ccggcgatcg gcgctgggcg acaatcggcg tctcgttgcg cagcccggaa acgcgagagg
     1501 cgctcgcgcc gcaggataat ctctacacgc tcgctctccg cgacggagcc ggcgagaggc
     1561 tgcaaatcat cggcggcttg gccgcgtgcc tcgtcgcgcc ggaagacccg cggaaactcg
     1621 tcgaagccat ggcgcgtccg gaaacgcgga tcgtctcgct gaccgtcacc gagaaaggct
     1681 attgccacaa tccggcgacc ggcgagctcg acgaaagcca tccccatatc cgccatgacc
     1741 ttgccgagcg aagccggccg cgcaccgcga tcggcttcat cgtcgaagct ctcgccttgc
     1801 gccgcgagcg gcggctcgcc ccgttcacgc tgctctcttg cgacaatctg ccgaccaatg
     1861 gcgagacgct gaaacgcgtc ctgaccggct acgcggcgct ccgcgatcgc gaactcgccc
     1921 gcttcgtcgc cgacgcagtc gcctgccctt cgaccatggt cgatcgcatc gtacccgcaa
     1981 ccaccgacgc cgatcgcgat tcgattgccg ccgcgagcgg gctccgcgac gcttggccgg
     2041 tcgtcgccga gcctttcgcg caatgggtaa tcgaggaccg cttcccgtca ggccgcccgg
     2101 aatgggatcg cgccggcgcg atgttcgtca aagaggtcgc ggcgttcgaa atgatgaagc
     2161 tgcgcctgct caacggcgcg cattcgacgc tcgcttacct cggctatctc gccggctgcg
     2221 agacaatcgc cgagaccatg gccgcgcccg gcttcgcccg cctcgccgag gattttatgg
     2281 cggtcgaagc cgcgccgacc ctgccgccgc tgccgggcct cgatcttgcg gcctatcgcc
     2341 gcgatctgat cgcccgcttt tgcaatcctg ccttgcgcca ccgcacctgg cagatcgcca
     2401 tggacggctc gcagaaattg ccgcagcgcc tgctcaacac gatccgggcg cgcctcggcg
     2461 cgaacgcgcc ttttccaagg ttggcgctcg gcgtcgccgc ctggatgcgc tatgcgacag
     2521 gcgtcgacga aaaaggcggc gcgatcgatg tgcgcgatcc tctggcggcg cggatcaagg
     2581 agatgacgcg gggagtgagc gaggcttcgg cgctggtttc ggcctatttc cgtttggcgg
     2641 aagttttcgg cgcggatttg ccagcggacg ctcggttccg cagcgccgtg acgacggctc
     2701 ttgccgcgct tttgaccaag ggttcggcgc gcgtcgtcgc gggattcgaa gcaagcgcgg
     2761 cgtgaagcgg cgcgtacttt ccctgctccc cgcgaagcgc tacggcatgt acacatcttg
     2821 aagcgcggcg cgcctgagcc gtgctcgccg tcaaggccaa gcgcgcttcg
//
LOCUS       DATGJW010000488         6507 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_407864, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000488 DATGJW010000000
VERSION     DATGJW010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6507)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6507)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6507
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_407864"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..715)
                     /locus_tag="VKU03_14585"
     CDS             complement(<1..715)
                     /locus_tag="VKU03_14585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267000.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HLJ72543.1"
                     /translation="MSFLRLYRRAFAQLAAARGAAFALAIANILVAAAQFAEPILLGR
                     IVDKLTAARAAMRTPQWRDVALPLAAWVGFGLFVILASVIVALNADRLAHRRRLAVMA
                     AYFEHALHLPLSFHVRAHSGRLLKVMIEGADGLFTLWLSFFREHCASVAALFILLPLT
                     LFLNWRLGSLLLALAIVFGLMMNFVLRRTERRQGEVGRYSSDLAERVSDALGNVPVIQ
                     SFARVDEETRAVRGLIEALL"
     gene            873..1295
                     /locus_tag="VKU03_14590"
     CDS             873..1295
                     /locus_tag="VKU03_14590"
                     /inference="COORDINATES: protein motif:HMM:NF018684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysozyme inhibitor LprI family protein"
                     /protein_id="HLJ72544.1"
                     /translation="MKPLLVSAFLILLAGAAFADAPRPSQLACDTKKIGARDLADCLR
                     AAADRSDKELAGALAAAIKSIDTRPGLLPAQKARWKRSLNDSQALWQNWRDADCQDVA
                     PFEAGMSAKGGDPRLGCIIDHDVQRVDELKARYQQNGS"
     gene            complement(1231..2175)
                     /locus_tag="VKU03_14595"
     CDS             complement(1231..2175)
                     /locus_tag="VKU03_14595"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HLJ72545.1"
                     /translation="MATRSAASASDPWPGFWRRRLIAAILAAADAFPHPGQDDGKRIH
                     EARKTLKEARALAKLMTAVIGPPAYDALRELEAARRRLGRARDLDVMAEALASLRGPL
                     DDRLIDTLGEAIAGERDAARAAHREIDAAAQIARLRALARAVDAWDLSAFDLFDLARS
                     LRDLYRAARRRGKKALADADPRELHELRVAIVDLGHCFDAMAPAWPAHFAAFAHEFRR
                     LREQLGRHNDLTVLGDFARSRDDIAPAEKGALSRAVERRQKRLAKRAQRFFDRLFAER
                     PNALFERVAAYMENPTASMSRSADSGPSAHRPAARRGR"
     gene            complement(2179..3816)
                     /locus_tag="VKU03_14600"
     CDS             complement(2179..3816)
                     /locus_tag="VKU03_14600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403315.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA synthetase"
                     /protein_id="HLJ72546.1"
                     /translation="MALSPYDIDLDRNPANFQPLTPLTFLERAAAVFPDHIAIIHGAL
                     RRNYRDFYARSRRLASALARRGFKRGDTVSALLANTPAMLECHYGVPMCGAVLNTLNT
                     RLDAAAIAFILDHGEAKALIVDREFSKLARDALAKCSVRPLIVDYDDPEYSGPGDRLG
                     ALDYEALLAEGDENFAWTAPTDEWDAIALNYTSGTTGDPKGVVYHHRGASLLAVGNVV
                     TADLGRHPMYLWTLPMFHCNGWCFPWSISVKAGTHVCLRQVRAKPMYDLIARHKVTHL
                     CGAPIVMSTLLNAPAGEKQPLPHVVRFFTAAAPPPASVLAAMKEAGFEVTHLYGLTET
                     YGPAVVNDWREAWSALSGPDQAALKARQGVRYHALEALDVLDPDTMRPAPADGETLGE
                     VMFRGNVVMKGYLKNKAATDKAFAGGWFHSGDLGVKHPDGYIQLKDRSKDIIISGGEN
                     ISSIEVEDALFQHPAVQLAAVVARPDEKWGETPCAFVELKPGMQASADELIAWCRERL
                     ARYKCPRHVVFSEIPKTSTGKVQKFVLRERARAIGQD"
     gene            complement(3879..4313)
                     /locus_tag="VKU03_14605"
     CDS             complement(3879..4313)
                     /locus_tag="VKU03_14605"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HLJ72547.1"
                     /translation="MTLQPDSIGARGALARQIPSFALIGALGFCVDAGLTVLIASGLG
                     VSPFLARLPAVVAATVATFLLNRAFTFGGRAFWLREFARYVLVAAGGQALNYAVYAAA
                     LTALGALGFGQAPAAITFSVACGAGAAMFLTFAGFRFFAFGP"
     gene            complement(4369..5127)
                     /locus_tag="VKU03_14610"
     CDS             complement(4369..5127)
                     /locus_tag="VKU03_14610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HLJ72548.1"
                     /translation="MVLDSSFYAAAVPAVILLGLSKGGFSGLNGLAMPIISLVISPVK
                     AAAILLPILIVQDWVSVWAFRHDWEKRNLLILLPASLIGVFLGWLLAAHVSEALVRLA
                     VGIISVGFVAYIVARALSGEPKPTEAKVAPGIFWGAVAGFTSFISHAGAPPFQVYVMP
                     QRLKPRLFAGTATMFFAAVNLLKVPPYFALGQFSHENLKAAATLFPLAILSTIAGVWL
                     IRRVDPERFYTFIYALTFLVGLRLVWEGLAELFV"
     gene            5238..>6507
                     /locus_tag="VKU03_14615"
     CDS             5238..>6507
                     /locus_tag="VKU03_14615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763407.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HLJ72549.1"
                     /translation="MPSRYDRVYAEWRADPQAFWAKAAQEIDWISPPRRIFDAEAGVY
                     GRWFPDATCNTCFNALDRHVAAGRGEQTAIIYDSPVTGAKRRISYAELLEETATLAAV
                     LSSLTVEKGDRVVIYMPMIPQALAAMLACARIGAIHSVVFGGFAARELAARIDDAKPK
                     LILAASCGVEPGRIVAYKPLLDAAIELSAHKPATCLVLQRPQAKAAMTAGRDVDWDEA
                     DAAAKAAGKRADCVELAATDPLYILYTSGTTGKPKGVVRDNGGHMVALKWSMANVYGV
                     APGETFWTASDIGWVVGHSYIVYGPLLHGCASVLYEGKPVGTPDAGAFWRVIDEHKVV
                     SFFTAPTAFRAVRKEDPEGALLKNYSTASLRTLFLAGERADPETLAWAGRLLNVPVVD
                     HWWQTETGWPIVANPVGLGLLPIKPGSPTVP"
BASE COUNT         1181 a         2100 c         2253 g          973 t
ORIGIN      
        1 cgagcagcgc ctcgatcagg ccgcggacgg cgcgcgtctc ctcgtcgacg cgcgcgaaac
       61 tctggatcac gggaacgttg ccgagcgcgt cggagacgcg ctcggcgaga tcggagctgt
      121 agcggccgac ctcgccttgc cgccgctcgg tccgccgcaa gacgaaattc atcatcaggc
      181 cgaaaacgat cgccaaggcg aggagcagcg agccgaggcg ccagttgaga aagagcgtca
      241 gcggcagcag gatgaacaaa gccgcgacgc tggcgcaatg ttcgcgaaag aacgacagcc
      301 acagggtgaa caggccgtcg gcgccctcga tcatcacctt cagcagccgg ccggaatggg
      361 cgcggacatg gaagctcagc ggcagatgca gcgcatgctc gaaataggcg gccatgaccg
      421 ccaggcggcg gcgatgcgcc aggcggtcgg cgttgagcgc gacgatcacg ctggcgagga
      481 tgacgaagag cccgaagccg acccaggcgg cgagcggaag cgcgacgtcc cgccattgcg
      541 gcgtccgcat cgccgctcgc gcggcggtga gcttgtcgac gatgcggccg agcagaatcg
      601 gctcggcgaa ttgcgccgcg gcgacgagaa tattggcgat ggccagggcg aaggctgcgc
      661 ccctggccgc ggcgagttgc gcgaaagcgc gcctgtagag gcgaaggaag gacatcggat
      721 gcgccggcct tgaccgcgcc gcttatcgag gagaggcggc gccgatgcaa gctcccgcaa
      781 ggccggaccg taaaaaacgc cgcaacattg acagagcgtc ctccaatgcg gttattgccg
      841 gcaaagctcg cctcgtcgtc agccgtgggc ccatgaaacc gcttctcgtt tcggcgtttt
      901 tgattcttct ggccggggcg gctttcgccg acgcgccacg cccgtcgcaa ctggcctgcg
      961 acacgaagaa aatcggcgcc cgcgatctcg ccgactgcct tcgcgccgcc gccgaccgca
     1021 gcgacaagga actggccggc gcgctcgccg cggcgatcaa gagcatcgat acgcgtccag
     1081 gtctgctgcc ggcgcaaaag gcgcggtgga agcgctcgct caatgactcc caggcgctgt
     1141 ggcagaattg gcgcgacgcc gattgccagg atgtcgcgcc gttcgaggcc ggcatgtcgg
     1201 ccaagggcgg cgatccgcgc cttggctgca tcatcgacca cgacgtgcag cgggtcgatg
     1261 agctgaaggc ccgctatcag cagaacggct catagaagcc gtcggattct ccatataggc
     1321 ggcgacgcgc tcgaacagcg cattcggccg ctcggcgaaa agccggtcga agaaccgttg
     1381 agcgcgcttg gcgagacgct tttgccgccg ctcgacggcg cgcgacagag cccctttttc
     1441 ggcgggcgcg atatcgtcgc gggacctggc gaagtcgccg aggactgtca aatcgttgtg
     1501 tcggccgagc tgttcgcgca ggcggcggaa ttcatgcgcg aaggcggcga aatgcgccgg
     1561 ccaggccggc gccatcgcgt cgaagcaatg gccaagatcg acgatcgcga cccgcaattc
     1621 gtgtaattcg cgcggatcgg cgtcggccaa ggcttttttg ccgcgccgcc gcgccgcccg
     1681 ataaaggtcg cgcagcgagc gggcgaggtc gaagaggtcg aacgccgaga ggtcccaggc
     1741 gtcgacggcg cgggcgagcg cgcgcaagcg cgcgatttgc gccgccgcgt cgatctcgcg
     1801 atgcgcggcg cgcgccgcat cgcgctcgcc ggcgatcgcc tcgccaagcg tgtcgatcag
     1861 ccggtcgtcg agagggccgc ggaggctggc gagcgcctcg gccatgacat cgagatcgcg
     1921 cgcgcggccg aggcggcggc gggccgcttc gagctcgcgc aaagcgtcat aagccggcgg
     1981 tccgatcacc gccgtcatca gcttggcgag ggcgcgcgcc tccttgagcg ttttgcgcgc
     2041 ttcgtggatt cgcttgccgt cgtcctggcc gggatggggg aaagcgtcgg cggcggcgag
     2101 gatcgcggcg atcagccggc gccgccagaa gcccggccac ggatcgctgg cgcttgccgc
     2161 gctgcgcgtc gccatcgcct agtcctggcc gatcgcgcgc gcgcgctccc gcagcacgaa
     2221 tttctgcacc ttgccggtcg aggttttcgg gatttcgctg aacacgacat ggcgcgggca
     2281 tttgtagcgg gcgaggcgtt cgcggcacca agcgatcagc tcgtccgcgc tcgcctgcat
     2341 gccgggcttg agctcgacga aagcgcaagg cgtctcgccc catttctcgt cgggccgcgc
     2401 cacgaccgcg gcgagctgga cggcgggatg ctggaacaac gcgtcctcga cttcgatcga
     2461 ggaaatattc tcgccgccgg aaatgatgat gtccttcgag cggtccttga gctggatata
     2521 gccgtcgggg tgcttgacgc cgaggtcgcc ggaatggaac cagccgccgg cgaaggcctt
     2581 gtcggtcgcc gccttgttct tgaggtaccc tttcatcacg acattgccgc ggaacatcac
     2641 ttcgccgagc gtctcgccgt cggcgggagc cggtcgcatc gtgtcgggat cgaggacgtc
     2701 gagcgcttcg agcgcgtgat agcgcactcc ctggcgcgct ttcagcgcgg cttgatccgg
     2761 accgctgagc gcgctccacg cctcgcgcca atcgttgacc acggccggcc cataggtctc
     2821 ggtgaggccg tagagatgcg tgacttcgaa acccgcctcc ttcatcgccg ccagcacgga
     2881 ggccggcggc ggcgccgcgg cggtgaaaaa gcgcacgaca tgcggcagcg gctgcttctc
     2941 cccggctggc gcgttgagca aggtcgacat gacgatcggc gcaccgcaaa gatgggtgac
     3001 cttgtggcgg gcgatcagat cgtacatcgg cttggcgcgc acctgccgca ggcagacatg
     3061 cgttccagcc ttgacgctga tcgaccaggg aaagcaccag ccgttgcaat ggaacatcgg
     3121 cagcgtccaa aggtacatcg gatggcggcc gagatcggcg gtcaccacat tgccgaccgc
     3181 caggaggctg gcgccgcgat gatggtaaac gacgcctttc ggatcgccgg tcgtgcccga
     3241 ggtgtaattg agcgcgatcg cgtcccattc gtcggtcggc gcggtccagg cgaaattctc
     3301 gtcgccttcg gcgagcagcg cctcgtaatc gagcgcgccg agcctgtcgc cggggcctga
     3361 atattcggga tcgtcgtaat cgacgatcag cggccggacc gagcatttcg ccagcgcgtc
     3421 gcgcgcgagt tttgaaaatt cccgatcgac gatcagcgcc ttggcctcgc cgtgatcgag
     3481 gatgaaggcg atggcggcgg cgtcgagccg cgtgttcagc gtgttgagca cggcgccgca
     3541 catcgggacg ccgtaatggc attcgagcat ggccggcgta ttggcgagca gggccgagac
     3601 cgtgtcgccg cgcttgaagc cgcgccgggc gagcgccgag gcgaggcggc gcgagcgggc
     3661 gtagaaatcg cggtaattgc gcctcaacgc gccatggatg atggcgatgt gatcgggaaa
     3721 aaccgccgcc gcgcgctcga ggaaggtcag cggcgtcagc ggctggaaat tggctggatt
     3781 gcggtcgagg tcgatatcat aaggcgacaa agccatggcg cggccccctt gtcggcggcc
     3841 atcataacca agccgaggcg aagcgcgaga ctgccccttt aaggaccgaa ggcgaaaaac
     3901 cggaagccgg cgaaggtcaa aaacatcgcc gccccggcgc cgcaggcgac cgaaaacgtt
     3961 atcgccgccg gcgcctgccc gaatcccagc gcgccgagcg cggtcaaagc agcggcgtat
     4021 accgcgtaat tcagcgcctg gccccccgcc gcgaccagga cgtagcgcgc gaattcgcgg
     4081 agccagaaag cccggccgcc gaaggtgaag gcgcggttga gcaggaaggt cgcgaccgtg
     4141 gccgcgacga cggccggcag gcgggcgaga aatggcgaca cgcccaatcc ggacgcgatc
     4201 agcaccgtca ggccggcgtc gacgcaaaag ccgagggcgc cgatcagggc gaaagagggg
     4261 atttggcgcg ccagcgcgcc gcgcgcgccg atcgaatccg gttgaagggt cacgcccaaa
     4321 actcccaggc gaccggcgaa ggttgcgcat ggggccgtta atgaagtctt agacgaacag
     4381 ttcggccagc ccctcccaga ccaggcggag gccgaccagg aaggtcagcg cgtagatgaa
     4441 cgtataaaag cgctcggggt cgacgcgccg gatcagccag acgccggcga ttgtcgacag
     4501 gattgcgagc ggaaataggg tggcggcggc ctttaggttt tcgtgcgaga attgcccaag
     4561 cgcgaaatag ggcggcactt tcaggaggtt gaccgcggcg aagaacatcg tcgcggtgcc
     4621 ggcgaagagg cgcggcttga gccgctgcgg catcacatag acctggaagg gcggcgcgcc
     4681 ggcatggctg atgaagctgg tgaagccggc gacggcgccc cagaaaattc cgggcgcgac
     4741 cttcgcctcg gtcggcttcg gctcgccgct caaggcgcgc gccacgatat aggcgacgaa
     4801 gccgaccgat atgatcccaa cggccaggcg gaccagggct tcgctgacat gggcggcgag
     4861 cagccagccg agaaagacgc cgatcagcga ggccggcagc aggatcagca ggttgcgctt
     4921 ctcccagtcg tggcggaacg cccaaacgct gacccaatcc tggacgatga ggatcggcag
     4981 caagatggcc gcggctttga ccggcgagat gaccagggag atgatcggca tggccaagcc
     5041 gttgaggccg gaaaaaccgc ccttggagag gccgagcaaa atcaccgccg ggacggcggc
     5101 ggcgtagaaa ctcgaatcca ggaccatgcc gccccttccg aacctcttgg ccgaacgtcg
     5161 catctgccgc agattgcgtc gaggcgcagc gcggcgcaga acgcgtcaat gacagaagag
     5221 ccgcaaggga gagaaaaatg cccagccgct atgatcgagt ctatgcggag tggcgggccg
     5281 atccgcaagc gttttgggcc aaggccgcgc aggaaatcga ctggatttcg ccgccgcggc
     5341 ggattttcga cgcagaggcc ggcgtctatg gccgctggtt tcccgacgcg acctgcaaca
     5401 cctgcttcaa cgcgctcgac cgccatgtcg ccgccggccg gggcgagcag accgcgatca
     5461 tctatgacag cccggtgacc ggagcgaagc gtcggatcag ctacgccgaa ttgctggagg
     5521 agactgcgac gctcgccgcg gtcctctcct cgctcactgt cgaaaagggc gaccgggtcg
     5581 tcatctatat gccgatgatc ccgcaagcct tggcggcgat gctcgcttgc gcgcggatcg
     5641 gggcgattca ttccgtcgtt ttcggcggct ttgcggcgcg agagctcgcc gcgcggatcg
     5701 acgacgccaa gcccaagctg attctcgccg cctcgtgcgg cgtcgagcct gggcgcatcg
     5761 tcgcctataa gccgctgctc gacgcggcga tcgagctcag cgcgcataag ccggccacct
     5821 gcctggtgct gcagcgtccg caggccaaag cggcgatgac cgccggccgc gatgttgatt
     5881 gggacgaggc cgacgccgcc gctaaagcgg ccggaaaacg cgccgattgc gtcgagctcg
     5941 ccgccaccga cccgctctat atcctttaca cgtccggcac gacgggcaag ccgaagggcg
     6001 tggtgcgcga caatggcggc catatggtcg cgctcaaatg gtcgatggcg aacgtctatg
     6061 gcgtcgcgcc cggcgagacg ttctggaccg cctcggacat cggctgggtc gtcggccact
     6121 cctatatcgt ttacgggccc ttgctccatg gctgcgccag cgtgctctat gagggcaagc
     6181 cggtcggcac gcccgacgcc ggcgcgttct ggcgcgtcat cgacgagcac aaggtcgtct
     6241 cgttcttcac cgcgccgacg gctttccgcg ccgtgcgcaa ggaggatccg gaaggcgcgc
     6301 tgctcaagaa ctattcgacc gcatcgctcc gtaccctctt cctcgccggc gagcgcgccg
     6361 atcccgaaac gctcgcttgg gccggtcggt tgctcaacgt cccggtcgtc gatcactggt
     6421 ggcaaaccga gaccggctgg ccgatcgtcg ccaatccggt cggcctcggc ctgttgccga
     6481 tcaagcccgg ctcgccgacg gttccca
//
LOCUS       DATGJW010000489         3806 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1026425, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000489 DATGJW010000000
VERSION     DATGJW010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3806)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3806)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3806
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1026425"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..256)
                     /locus_tag="VKU03_14620"
     CDS             complement(<1..256)
                     /locus_tag="VKU03_14620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003603905.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLJ72550.1"
                     /translation="MAIEAARNRKRPELRRSPRKQATLRGRYMLSDGREYPCRTFDIS
                     AGGVAIVSAVKGKLGERVVLYLDRIGRLEGTVARHFGGGFA"
     gene            619..795
                     /locus_tag="VKU03_14625"
     CDS             619..795
                     /locus_tag="VKU03_14625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72551.1"
                     /translation="MLTPWLIAAALGLAALVLFGSIERNLQNRDAIPTGPTTHHASIL
                     CPPQTGSGRAVVCR"
     gene            938..2656
                     /locus_tag="VKU03_14630"
     CDS             938..2656
                     /locus_tag="VKU03_14630"
                     /EC_number="6.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018453141.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="long-chain-fatty-acid--CoA ligase"
                     /protein_id="HLJ72552.1"
                     /translation="MERIWLKSYPAGVPADIDVAQYPSVVALLDEAFVKYRDLPAYIS
                     MGATLTYGEIDDLSKIFAAWLQSQGLRPGDRLALMMPNVLQYPVAMAAALRAGLIVVN
                     VNPLYTARELSHQLQDSGAKAIVILENFAATLEKALAGTAVERIVLTGIGDLMGFPKG
                     LLTNFVVRRIKKMVPAYDLPHAVRFKAAIAAGRRSRFTPPPIAPKDVAVLQYTGGTTG
                     VAKGAMLLHETLVAAALSAEAWMQPGLKRKAVSGQKTVVCALPLYHVFAFVVCALLGM
                     RTGALNILIVNPRDLAGVIKEISKYKFNMFPAVNTLFNALANHPDFAKLDFSGLFNST
                     GGGMAVQEAVAKKWLAVTGCPIVEGYGLSETAATGTCNPTDSTGFTGTIGLPLPNFDI
                     RIIDDSGADVPLGSPGEIAIKGPQVMSGYWGRPDETAKAFTADGFFKTGDIGVMDARG
                     YIKLVDRKKDMILVSGFNVYPNEIEEVVAHHPGVAECAAVGIPDKNSGEAVMLFVVKR
                     DPDLTAEALAAFCAAELTGYKRPRSIEFRKELPKTNVGKILRRALRDEAAAMMKDAAA
                     SKASAA"
     gene            complement(2772..3017)
                     /locus_tag="VKU03_14635"
     CDS             complement(2772..3017)
                     /locus_tag="VKU03_14635"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6489 family protein"
                     /protein_id="HLJ72553.1"
                     /translation="MKITFDCDCTPAEAREFFGLPDVRSMQAKVMEEIEKRIMAQLDS
                     LSPESVLRGWMSFAPDQFQTFFKNMMNYPGASKTGGG"
     gene            complement(3124..3672)
                     /locus_tag="VKU03_14640"
     CDS             complement(3124..3672)
                     /locus_tag="VKU03_14640"
                     /inference="COORDINATES: protein motif:HMM:NF018213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative glycolipid-binding domain-containing
                     protein"
                     /protein_id="HLJ72554.1"
                     /translation="MIVASMLWRRIDTPGHDACRLEKTPSGWSLRGVAVFRQAGEVAT
                     LSYTVNCDGDWKTVSGRIAGAVGERAVDDLIVRENGGWRLNGDPAPGLGRLQDLDLSF
                     TPATNVLQLKRVELPIGRPVRLPAAWFDLATRRLSELEQIYERRAALALSYRAPGVGY
                     EGLLELAPNGFIRLYPGLWEAE"
BASE COUNT          704 a         1277 c         1164 g          661 t
ORIGIN      
        1 cggcgaaacc gccgccgaaa tggcgcgcga ccgttccttc gagtcttcca atacgatcga
       61 gatagaggac gacgcgctcg ccgagcttgc ccttgactgc gctcacaatg gcgacgccgc
      121 cggcggaaat atcgaaagtc cggcagggat attcacggcc gtcggagagc atgtagcggc
      181 cacggagggt cgcttgcttg cgcgggctgc gccgaagctc cggccgcttg cgattgcgcg
      241 ccgcctcgat agccatgctc ggcaaccctt gacccgtcgt gccttccgat cgcgccaagc
      301 ttagcgaagg gagcgcaaac aaaggttaaa cccaccggta aaacgctcgc gagcgcccgt
      361 ctatggcgga acctttttcg aaccgcgcgc tgtttatttt tttcgggcga cgtcgggcct
      421 gacgccggcg aaggtctctt tgaagtcggg agcccgcggc cggcgatcgg cgcccgccgg
      481 tcggaacagc ggtcaggctg gtccgttgaa catgcgtcga gaccgtacgc cttcaagatt
      541 ggctgcaggc cagcaatgcc caccgaaccc cgacccagaa gcaaagttta tcgcacgcct
      601 gacgacaacc acagcgagat gctgacgcct tggctgatcg ccgccgcgct gggcctcgcc
      661 gccctggttc ttttcgggtc gatcgaacgg aacctgcaga atcgcgacgc catcccgacc
      721 ggcccgacga cgcatcacgc gagcatcctt tgtccgcctc agaccggatc gggccgcgcg
      781 gttgtctgcc gctaagactc gccttggcgg ccctcgtcct cacgcaaatg tgcgaaaccg
      841 ccgcccggcc gcgccgcatg ttgacgaaat cgcccgccct ttgaccgcag cggcaatgcc
      901 tttaagctgc cggcaaaagc aaggcgagag gaaacccgtg gagcgcatct ggctgaaaag
      961 ctatccggcc ggcgtgccgg ccgatatcga cgtcgcgcaa tatccgtcgg ttgtcgcgct
     1021 gctggacgaa gccttcgtca aatatcgcga tctccccgcc tatatctcca tgggcgcgac
     1081 gctgacttac ggcgagatcg acgatctttc caagattttc gccgcctggc tgcaaagcca
     1141 aggcttgcgg cccggcgacc gcctggcgct gatgatgccg aacgtcctgc aatatcccgt
     1201 cgccatggcg gcggccctgc gggccggcct catcgtcgtc aacgtcaacc ctctctacac
     1261 ggcgcgcgaa ctctcccatc agctccagga ttccggcgcc aaggcgatcg tcattctcga
     1321 aaatttcgcc gcgacgctcg aaaaggcgct tgccggaacc gcggtcgaac gcatcgtttt
     1381 gaccggcatc ggcgacctga tgggctttcc caaggggctc ctcaccaatt tcgtggtgcg
     1441 gcgaatcaag aagatggttc cggcctacga tctgccgcat gcggtgcgct tcaaggcggc
     1501 gatcgccgcc gggcggagat cgcgcttcac gccgccgccg atcgcgccga aggacgtcgc
     1561 ggttctccaa tataccggcg gcacgacggg cgtcgccaaa ggcgcgatgc tgctgcacga
     1621 aaccttggtc gccgccgccc tttccgcgga agcgtggatg cagccgggcc tcaagcgcaa
     1681 ggcggtcagc ggccaaaaga cggtcgtgtg cgccctgccc ttgtaccatg tcttcgcctt
     1741 cgtggtctgc gcgctgctcg gcatgcggac cggcgcgttg aacatcctga tcgtcaatcc
     1801 acgcgatctt gccggcgtga tcaaggagat ttcgaaatac aagttcaaca tgtttccggc
     1861 agtcaacacg ctgttcaacg cgctggcgaa ccatccggat ttcgccaagc tcgacttttc
     1921 gggactgttc aactcgaccg gcggcggcat ggcggtgcaa gaggccgtcg ccaagaaatg
     1981 gctcgccgtg accggatgcc cgatcgtcga aggctatggc ctatccgaga ccgcggccac
     2041 cggaacctgc aatccgacgg actcgaccgg ctttaccggc acgatcggcc tgccgctgcc
     2101 caatttcgac atccggatca tcgacgattc cggcgccgac gtcccgcttg gctcgcccgg
     2161 cgagatcgcc atcaaaggcc cgcaggtcat gtccggttac tggggccgcc cggacgagac
     2221 ggcgaaagcc ttcaccgccg acggcttctt caaaaccggc gatatcggcg tcatggacgc
     2281 gcgcggctac attaaattgg tcgaccgcaa gaaggacatg attctcgtct cgggcttcaa
     2341 cgtctatccg aatgagatcg aagaggtcgt cgcccatcat cccggcgtcg ccgaatgcgc
     2401 cgcggtcggc atacccgaca agaattccgg cgaggcggtt atgctgtttg tcgtcaagcg
     2461 cgatcctgat ctgaccgccg aggcgctcgc cgctttttgc gcggccgagc tcaccggata
     2521 caagcggccg cgctctatcg aattccgtaa agagctcccc aagaccaatg tcggcaaaat
     2581 cctgcgccgc gccttgcgcg acgaggcggc cgcaatgatg aaggacgccg ccgcgagcaa
     2641 ggcgtcggcg gcttaaccgc cgcgtcattg cgagaagcga aagcgacgag gcaaatccgt
     2701 tcgtagagcg acgccccatc aatggatcgc ttcgcttgcc tggcaatgac cgcattcgga
     2761 ctagccaagg actaaccgcc gcccgtcttg ctcgcgcccg gatagttcat catgttcttg
     2821 aaaaaggtct ggaattgatc gggcgcgaag gacatccaac cgcgcaggac cgattccggc
     2881 gacaagctgt cgagctgcgc catgatgcgc ttctcgatct cctccatcac tttcgcttgc
     2941 atcgatctga catcgggcag gccgaaaaat tcgcgcgcct cggcgggcgt gcagtcgcaa
     3001 tcgaaggtga tcttcatgcc tcggcgctcc catcgccggc gcagcgccgc cctgctggcg
     3061 cgcgctgtgc gtcggatgag cttgaccggg ccttgatcct cccgtcaagc cggcggcgcg
     3121 cggctattcc gcctcccaaa gacctggata gagtcggatg aacccgttcg gcgcgagttc
     3181 gagcaggcct tcgtaaccga cgcccggcgc gcgataggaa agagcaagcg cggcgcgccg
     3241 ctcgtagatt tgctcaagct cgctcaatcg gcgcgtcgcc aaatcgaacc acgccgcagg
     3301 cagccggacc ggccgcccga tcggcagttc gacgcgcttc agttgcagga cgttggtcgc
     3361 cggcgtgaag ctcaagtcga gatcctggag tctcccaagc cccggcgctg gatcgccgtt
     3421 gaggcgccaa ccgccgttct cgcggacgat cagatcgtcg acggcgcgtt cgccgacggc
     3481 gccggcgatg cggccggaca cggttttcca atcgccgtcg caattcaccg tataggagag
     3541 ggtcgcaact tcgccggcct ggcgaaagac ggcgacgccg cgcaagctcc agccggatgg
     3601 cgtcttttca agccggcagg cgtcatggcc gggcgtatcg atccgccgcc acagcatcga
     3661 ggcgacgatc atgcggcccc gcctcgggcg ccctgcccca ggaacgtcct ccggcgcgcg
     3721 gcgttgcgcc ggcagaaagt caggctcagg cgccgttcgc cggcgcaaag gagacgagcc
     3781 gtggtcaatc gcggcccaga gaacag
//
LOCUS       DATGJW010000490        11178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_408250, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000490 DATGJW010000000
VERSION     DATGJW010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11178
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_408250"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..176)
                     /locus_tag="VKU03_14645"
     CDS             complement(<1..176)
                     /locus_tag="VKU03_14645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043295 - glutathione binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione S-transferase N-terminal
                     domain-containing protein"
                     /protein_id="HLJ72555.1"
                     /translation="MVTLYHHPFCPHSRFVRLVLAEFGVEPELIEEKPWERRREFLLL
                     SPEGATPLMVEPEG"
     gene            complement(288..926)
                     /gene="pncA"
                     /locus_tag="VKU03_14650"
     CDS             complement(288..926)
                     /gene="pncA"
                     /locus_tag="VKU03_14650"
                     /EC_number="3.5.1.-"
                     /EC_number="3.5.1.19"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003606982.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional nicotinamidase/pyrazinamidase"
                     /protein_id="HLJ72556.1"
                     /translation="MDEVFTPRAGDALIVVDMQYDFMPGGALAVAGGDAIVPLVNRIA
                     AKFANVVLTQDWHPPGHASFASSHPGAKPFDTIRLSYGEQVLWPDHCVQKSRGAELHR
                     GLDIGQAILVLRKGANRDVDSYSAFVEADGASRTGLAGFLRERGIGRVFLCGLATDYC
                     VGFSALDARSAGFDALVVDDACRAIDLAGSLAAAWAKMEAAGVRKISAAQLG"
     gene            complement(937..1878)
                     /locus_tag="VKU03_14655"
     CDS             complement(937..1878)
                     /locus_tag="VKU03_14655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003512445.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbhN family protein"
                     /protein_id="HLJ72557.1"
                     /translation="MMRARDYAWTVVGLSAVAFSLWLLVRELRGLSLDQVVAGFSAIP
                     LHRWLLAFCSTIVAYAALAWYDRIGLSHLGHKLPWGFISIVSFTTYALSHNIGASILS
                     GAVVRYRAYSTKGLNAREVGLLVAFCSFTFILGELLLGGLILTFAPEIVDRWSQFPHW
                     AARLAGLVMLGMVGLYALGSLRHFPAMTIGGFELVYPRPKILVRQLLAAPIEIIGAAG
                     IIYFTLPVAGNPGFVAVLGVFLGSFAVALASYAPGGIGVLEFCFLKGMPDVPAADVLA
                     ALIAFRLLYLLIPLAFAVVAVFQFERGRLGEILRLHF"
     gene            2086..3048
                     /locus_tag="VKU03_14660"
     CDS             2086..3048
                     /locus_tag="VKU03_14660"
                     /inference="COORDINATES: protein motif:HMM:NF013247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class IV"
                     /protein_id="HLJ72558.1"
                     /translation="MQEQGESIGGSQDYVGDPRNDGVLISVDGALTPRDEAKVSVFDS
                     GFVLGDGVWEGLRLIDGKIAFLDKHLDRLWDGAKMLRIDIGLSRQALTARLFETLDAN
                     KMTDGVHIRLMVTRGKKRSPYQDPRLTLGKATIVIIPEWKLPLPAMRARGLKLFTVHV
                     RRTGPAEQDQKLNSHSKLNCVLACIQAMEAGADEGLMLDDRGFVATCNSTHFFIVRKG
                     EVWTSSGGYCLGGITRDAVLRAARRAGIPAYERDFSLFDVYGADEAFCTGTFAGLTPV
                     AMIDGRPIGDASARSADDASGPLTRRLRALYKALEAEEAQGRAA"
     gene            3049..3813
                     /locus_tag="VKU03_14665"
     CDS             3049..3813
                     /locus_tag="VKU03_14665"
                     /inference="COORDINATES: protein motif:HMM:NF040370.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72559.1"
                     /translation="MNEPLRIAMWSGPRNISTAMMRAFGNRRDCAASDEPFYAAYLVA
                     TGLDHPMRDDVIASQPSDWRAVVAELTGPVPGGKPIWYQKHMTHHMVEGFGRDWIDGF
                     ANAFLIRAPETVIASYAQKREEFSLEEIGLPAQLDLFERAAERLGRAPPVIEGNDVLA
                     DPRGALATLCSALGAPFDEAMLAWPAGRKPFDGVWAPVWYDAVEKSIGFGPPQNEARF
                     DDLPDRLKPLAEAARPYYEKLAQHRLFARSDVTRLI"
     gene            4017..4718
                     /locus_tag="VKU03_14670"
     CDS             4017..4718
                     /locus_tag="VKU03_14670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72560.1"
                     /translation="MAPLNYTTALIVGAGSGLSASLARLFAREGLRVALAARNAEKLG
                     DLKSSLGAEAFACNAADAGDVARLFEAVEKRLGAPDIVVYNASGRLRGPFVDLNPSEV
                     ERAIAVSAFGGFLVAQQAARRMLAKGRGAILFTGASASVKGYAQSAPFAMGKFALRGL
                     AQSMARELAPQGIHVAHFVIDGAIRNPGRSEAPDKPDSMLDPDAIAETYLHILRQPRS
                     AWTWEVELRPWVEKF"
     gene            4976..6469
                     /locus_tag="VKU03_14675"
     CDS             4976..6469
                     /locus_tag="VKU03_14675"
                     /EC_number="3.6.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404160.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003678 - DNA helicase activity [Evidence
                     IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="replicative DNA helicase"
                     /protein_id="HLJ72561.1"
                     /translation="MATVEQFPARLEPRSAEAVARIAPQNIEAEQALLGAILINNDAF
                     DRVSDFLQSEHFSHELHRRIYEMAGQLIRAGKLASPITVKGFLGEHDLGGVTVQQYLA
                     RLVAEATTIINAHDYGRTIHDLALRRELIRIGEDIVNVAYDSPVDSTPREQIEDAERR
                     LYQVAEGGRYDGGFARFADALKTAIDMAAKAYERDGHLSGIATGLVDLDHKMGGLQPS
                     DLVILAGRPGMGKTALATNIAFSIAKAYQGETRADGSRQTLDGGIVGFFSLEMSAEQL
                     ATRIIAEQSGVASNKIRRGDITEQDFHRISMAMRDMQSIPFYIDQTGGISIAQLTARA
                     RRLKRQRGLDLLVVDYLQLLSGSKTRGDNRVQELTEITTGLKALAKELNVPILALSQL
                     SRQVEQRDDKRPQLSDLRESGSIEQDADVVLFVFREEYYLKNREPRPGTEEYTAWQNE
                     MERAHGRAEVIIGKQRHGPTGTVELQFEAELTRFGNLARDEQMPERY"
     gene            6669..7853
                     /gene="alr"
                     /locus_tag="VKU03_14680"
     CDS             6669..7853
                     /gene="alr"
                     /locus_tag="VKU03_14680"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019404159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HLJ72562.1"
                     /translation="MLSLTPMTSSSDAGPALTAAGGVLTIDLSALADNWRRLAERVAP
                     AECAGVIKANAYGTGLEPAARALFAAGCRTFFVAQVAEGVRARAALGAAARIFVLNGL
                     QARAEPADYVAHRLAPILGSPEELARWAPFAADGPACAIHLDTGMSRLGFASLDALEA
                     ALAGYDHSRIALIMSHFVSSEEQGDPLNDRQIALFDKARALLPGVPASLANSSGIFLA
                     GRPFYDLVRPGYALYGGNPTPATPNPMRSVVRLEARIQQVRQIEPGASVGYNAQWMAK
                     RSTRLATLLIGYADGLPRSAGATDQRSGADVIIAGRRCPLVGRVSMDLCVADVTDIPE
                     SAPRPGDSAVILGEEISVDELGARAGTIGYQILTSLGARYRREYIPFSPQSGEKVARS
                     AG"
     gene            8021..9409
                     /gene="radA"
                     /locus_tag="VKU03_14685"
     CDS             8021..9409
                     /gene="radA"
                     /locus_tag="VKU03_14685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009736051.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HLJ72563.1"
                     /translation="MAKGKSVFVCQNCGAITRRWQGKCESCGEWNTIVEEAEPSGIGA
                     AAARRAEKGRAFALEGLEGGPAQEARTVIGLNELDRVAGGGLVPGSVALIGGEPGIGK
                     STLLIQACAALARRNGRVVYISGEESTAQVRLRAGRLGLADAKVELAAETSVEDIVAT
                     LSLGEPPSLVIIDSIQTMWSQAVESAPGTVTQVRASAQSLIRFAKSSGAAVILVGHVT
                     KDGQIAGPRVVEHMVDAVMSFEGEGSHAFRILRATKNRFGPTDEIGVFEMTGGGLSEV
                     VNPSALFLAGRDASSPGAAVFAGIEGARPLLVEFQALVAPTTLGTPRRAVVGWDSSRL
                     AMILAVLEAHGGLKLGQYDVYLNVAGGLKIIEPAADLAAAAALASSLVGASLPADAVY
                     FGEIGLSGAVRPVAHAALRLKEAAKLGFRRAVTPPPPKERDETVKIARTEISSVASLI
                     AEIAAMGVKSAR"
     gene            9510..10235
                     /locus_tag="VKU03_14690"
     CDS             9510..10235
                     /locus_tag="VKU03_14690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266270.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CvpA family protein"
                     /protein_id="HLJ72564.1"
                     /translation="MPSYLDLGVIVIVLISAFLSMVRGFTREVLAIGSWVAAAAAAYF
                     FYPYLMPYATPYIHKEIIAQAVCAAAVFFVTLVVVSTFTVRISDAILDSKIGALDRSL
                     GFLFGVARGFLLAVVAFAIFNWLVADKQQPQWVKDAKTRPILTATADRVIAMLPEDAE
                     HTIENWLKSGKAGVAPDQAPPPDEQQGTTPSGAAPATSPAAPAPAPAGSTPQKKTDAS
                     PDASSKQKLDAIINGKSGATKQN"
     gene            10281..>11178
                     /locus_tag="VKU03_14695"
     CDS             10281..>11178
                     /locus_tag="VKU03_14695"
                     /EC_number="2.4.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175479.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004044 - amidophosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidophosphoribosyltransferase"
                     /protein_id="HLJ72565.1"
                     /translation="MISEEPFLDLDADRLREECGVFGIFGHSDAAAITALGLHALQHR
                     GQEAAGIVAFDGGRFHSERRLGLVGDHFSKASTIERLPGSAAVGHVRYSTTGETILRN
                     VQPLFAELDGGGLAVAHNGNLTNGLTLRRELVRQGAICQSTSDTEAILHLVARSRRPT
                     IMERFIEALRQIEGAYALVALTNKKLIGARDPLGIRPLVLGEFEGCPILASETCALDI
                     IGAKFVRDVENGEVVVISQGGVESLRPFPPQRMRPCIFEYIYFARPDSIVHGRSVYDA
                     RKAMGAELAREAPADADVVVPVP"
BASE COUNT         1959 a         3690 c         3689 g         1840 t
ORIGIN      
        1 aaaccctccg gctcgaccat caagggcgtc gcgccttccg ggctgagcag caggaattcc
       61 cgccggcgct cccacggctt ctcctcaata agctccggct cgacgccaaa ttccgccagg
      121 accagccgga cgaagcgcga atgcggacag aaggggtgat gatagagcgt gaccatccaa
      181 cgcgaacttg tgctcgaaat gcgggcggct tctaagcagg agctttggat gcggccaatc
      241 tggttaaccg ggagtttagg cggctgaatt gacgaacgac gaagcgttca gccgagctgg
      301 gcggcgctga tttttctgac gccggccgcc tccatcttcg cccaggcggc ggcgagggag
      361 ccggcgaggt cgatggcgcg gcaggcgtcg tcgacgacga gggcgtcgaa gccggcggac
      421 cgcgcgtcga gcgccgaaaa gccgacgcaa tagtcggtcg cgaggccgca gagaaatacc
      481 cggccgattc cgcgctcgcg caggaagccg gcaagaccgg tcctggaggc gccgtcggcc
      541 tcgacaaagg cagaatagga atcgacgtcc cggttggcgc ccttgcgcag caccaggatg
      601 gcttggccga tgtcgagccc gcgatgcaat tcggcgccgc ggcttttctg cacgcaatga
      661 tccggccaaa ggacctgctc gccataggaa aggcggatcg tgtcgaaagg cttggcgccg
      721 ggatgggagg aggcgaacga ggcgtggccg ggcggatgcc aatcttgcgt cagcacgaca
      781 ttggcgaatt tcgcggcgat ccgattgacg agcggcacga tcgcgtcgcc gccggcgacc
      841 gccagcgcgc cgcccggcat gaaatcatat tgcatatcga cgacgatcag ggcgtcgccg
      901 gcgcgcggcg tgaaaacctc gtccacggac cttcctctaa aaatgcaggc gcaaaatctc
      961 gccgaggcgc ccgcgctcga attgaaacac cgcgaccacg gcgaaagcga gggggatcag
     1021 caaataaagc agccggaagg cgatgagcgc ggcgagaacg tcggccgccg gaacgtccgg
     1081 catgcctttc aagaagcaga attccaatac gccgatgccg cccggcgcat agctggcgag
     1141 cgcgacggcg aagctgccga gaaacacgcc gagtacggcg acaaagccgg gatttccggc
     1201 gacgggaagg gtgaaataga tgatgcccgc cgcgccgatg atctcgatcg gcgccgcaag
     1261 gagctgccga acgagaatct tcggacgcgg atagacgagc tcgaatccgc cgatcgtcat
     1321 cgccgggaaa tggcgcagcg aaccaagcgc gtaaagcccg accatcccca gcatgacgag
     1381 gccggcgagg cgcgccgccc aatgggggaa ttggctccag cggtcgacga tttcgggggc
     1441 gaaggtcaga atgaggccgc cgagcagcaa ctcgccgaga ataaaggtga acgagcagaa
     1501 cgcgacgaga agaccgactt cccgcgcatt caggcccttg gtggaatagg cgcggtagcg
     1561 cacgaccgct ccggagagaa tcgaggcgcc gatattgtgc gacagcgcat aggtcgtgaa
     1621 cgagacgatc gagataaagc cccagggcaa tttgtgcccg agatgggaga ggccgatgcg
     1681 gtcataccag gcgagggccg cataggcgac gatggtcgag cagaaggcga gcagccagcg
     1741 atgaagggga atcgccgaaa agccggccac gacctggtcg agcgacaggc cgcgcaattc
     1801 cctgaccagc agccacagcg aaaaggcgac ggctgagagc ccgacaaccg tccaagcgta
     1861 atccctcgcc cgcatgattc ccttgaaaac cggcgcgcct cgctgctttc cctgcacgag
     1921 ccgagagaga agggcaagcc gaagcgcgct tgcgaagacc cgatctttta tggaaagcac
     1981 aaggctccaa ggttgtttca gcgtccgcgg cgctgaagcc ttgccggaac gcgccggcgc
     2041 gctcatgctt cggcgcgaag ccgcgaccgc gatggaaacg aggcgatgca ggaacaaggg
     2101 gaatcgatcg gcggctcgca ggattatgtc ggcgatccgc gcaatgacgg cgtgctgatc
     2161 agcgtcgacg gcgcgctgac gccgcgcgac gaagcgaaag tgtcggtctt cgactcgggt
     2221 ttcgttttgg gcgacggcgt ctgggagggc ctgcgcctga tcgacggcaa gatcgcgttc
     2281 ctcgacaagc atctcgaccg gctatgggac ggcgcgaaaa tgttgcgcat cgacatcggc
     2341 ctttcgcgcc aggcgctgac cgcgcgtctg ttcgaaacgc tcgacgccaa caagatgacg
     2401 gacggcgtcc atatccggct gatggtgacg cgcggcaaga agcgctcgcc ctatcaggat
     2461 ccgcgcctga ccctcggcaa ggcgacgatc gtcatcatcc cggaatggaa gctgccgctg
     2521 ccggcaatgc gcgcgcgcgg cctgaaactt ttcaccgtcc atgtccgccg gacgggaccg
     2581 gccgagcagg atcagaagct caattcccac tccaagctca attgtgttct cgcctgcatc
     2641 caggcgatgg aggcgggcgc cgacgaaggc ttgatgctcg acgatcgggg cttcgtcgcc
     2701 acctgcaatt cgacgcattt cttcatcgtc cgcaagggcg aggtctggac ctcttccggc
     2761 ggctattgtc tcggcggaat cacccgcgac gcggtgctgc gcgcggcgcg ccgcgccggc
     2821 atccccgctt acgaacgcga cttctcgctg ttcgacgtct atggcgcgga cgaagcgttt
     2881 tgcaccggga cgttcgccgg cttgaccccg gtcgccatga tcgacggccg gccgatcggc
     2941 gacgcttcgg cgcgttcggc ggacgacgct tccggcccgc tgacgcggcg cctgcgggcg
     3001 ctttacaagg cgctggaggc cgaagaagcg caaggcaggg cggcttagat gaacgagccg
     3061 ctgcgcattg cgatgtggtc ggggccgcgc aatatttcca ccgcgatgat gcgcgcgttc
     3121 ggcaatcggc gcgattgcgc cgcgtcggac gagcctttct atgccgccta tctcgtcgcg
     3181 accgggctcg atcacccgat gcgcgacgac gtgatcgcct cgcagccgag cgattggcgc
     3241 gccgtcgtcg cggaattgac cggtccagtc cctggcggca agccgatctg gtaccagaaa
     3301 catatgaccc atcacatggt cgaaggcttt ggccgcgact ggatcgacgg cttcgccaac
     3361 gcctttctca tccgcgcgcc ggaaaccgtc atcgcctctt acgcgcaaaa gcgcgaagaa
     3421 ttctcgcttg aggaaatcgg cctgcccgcc cagctcgatt tgttcgagcg cgccgccgag
     3481 cggctcggca gggcgccgcc ggtgatcgaa ggcaatgacg tgctcgccga tccgcgcggc
     3541 gcgctcgcca cgctatgctc tgcgctcggc gcgccgttcg acgaggcgat gctcgcctgg
     3601 ccggcgggcc gcaagccttt cgacggcgtc tgggcgccgg tttggtacga cgcggtcgag
     3661 aagtcgatcg gcttcggccc gccgcaaaac gaggcgcgtt tcgacgatct gcccgatcgc
     3721 ctaaagccgc tcgccgaagc ggcgcggcct tactacgaga agctcgcgca gcatcggcta
     3781 ttcgcgcgca gcgacgttac acgcttaata tgatgagcgc tgcggcttac cctcatccgg
     3841 ccgcttcgcg gccaccttct cccgcaagcg ggagaaggga ttgtaatcgc ggtcgccgag
     3901 cgcggctccg gcgtcgatga cgcgcgcggc gcgccccctc tcccgcttgc gggagagggc
     3961 tggggtgagg gtgagcgttt gtccgaaggc aaaagcaaac gtcaaaggag agatcaatgg
     4021 ccccgctgaa ttacaccacc gccctcatcg tcggcgcggg ctcgggcctc agcgcctcgc
     4081 tggcgcggct ctttgcgcgc gagggcttgc gcgtcgcgct tgcggcgcgt aacgccgaaa
     4141 agctcggcga tctgaagtcc agcctcggcg ccgaggcctt cgcatgcaac gctgcggacg
     4201 ccggcgatgt cgcgcgtctg ttcgaagcgg tcgagaaacg gctcggcgcg ccggacatcg
     4261 tcgtctacaa cgccagcggc cgcttgcgcg ggccttttgt cgacttgaat ccgagcgaag
     4321 tggaacgcgc cattgcggtc agcgctttcg gcggtttcct cgtcgctcaa caagcggcgc
     4381 ggcgcatgct ggccaagggg aggggcgcaa tcctgttcac cggcgcttcg gcgagcgtca
     4441 agggctacgc ccaatccgcg cccttcgcga tgggcaagtt cgccctgcgc ggcctcgccc
     4501 agagcatggc gcgcgagctt gctccgcaag ggattcacgt cgcgcatttc gtcatcgacg
     4561 gcgccatccg caatcccggc cgaagcgaag cgccggacaa gccggattcg atgctcgatc
     4621 ccgacgccat cgccgagacc tatctgcaca tcctgcgcca gccgcgcagc gcctggacct
     4681 gggaggtgga gctcaggccc tgggtggaga aattctgagc gccgcggccg gcgcggcgcg
     4741 aaggccgcag tcaagcgcca attgcgggcc acatccagaa aaagcggccg cgtgggcata
     4801 atggggacaa ttgcggcttg cccccgccgc gccgtgttcc gcctcttcgc aaagacggac
     4861 aggtcgggca gatatttgcc gcgcctgctt ggtcgggcgt aggctaaaat tcgaacgaat
     4921 cgttttccag agtcggcgtt cgcgcgtttc ctcaaaaatc aaaaatcgaa gcatcatggc
     4981 gaccgtcgaa caatttccgg ctcggctcga accgcgttcc gccgaagccg tggcgcgcat
     5041 cgcgccgcaa aatatcgagg cggagcaggc gctgctcggc gcgattctga tcaacaacga
     5101 cgccttcgat cgcgtctcgg atttcctgca atccgagcac ttttcgcatg aattgcaccg
     5161 ccgcatctac gagatggcgg gccagctcat tcgcgccggc aagctcgctt cgccgattac
     5221 cgtcaaaggc tttctcggcg agcacgatct cggcggcgtc acggtccagc aatatctggc
     5281 gcgcctcgtc gccgaagcga cgacgatcat caacgcccat gattacggcc ggacgatcca
     5341 cgacctcgcc ttgcgccgcg agttgatccg gatcggcgag gacatcgtca atgtcgccta
     5401 cgattcgcca gtcgattcga ctccgcgcga gcagatcgaa gacgccgagc gccgcctcta
     5461 tcaggtcgcc gaaggcggcc gctatgatgg cggcttcgcg cgcttcgccg acgcgttgaa
     5521 aaccgccatc gacatggcgg cgaaagccta tgagcgcgac ggccatctca gcggcatcgc
     5581 caccggcctc gtcgatctcg atcacaaaat gggcggcttg cagccctccg atttggttat
     5641 cctcgccggc cggccgggca tgggcaagac cgcgctcgcg accaacatcg ccttctcaat
     5701 cgctaaggcg taccaaggcg agacccgcgc cgacggctcg cggcaaacgt tggacggcgg
     5761 catcgtcggt tttttctcgc tggaaatgtc ggccgagcaa ttggcgaccc gcatcatcgc
     5821 cgagcaatcg ggcgtcgcgt ccaacaagat caggcgcggc gacattaccg agcaggactt
     5881 ccatcgcatc tcgatggcga tgcgcgacat gcaatcgatc cccttctata tcgaccagac
     5941 cggcggcatc tcgatcgccc agctcacggc gcgggcgcgg cggctgaagc gtcagcgcgg
     6001 cctcgacctt ctcgtcgtcg actatttgca gcttctttcc ggctcgaaga cgcgcggcga
     6061 caaccgcgtt caggaattga ccgagatcac caccgggctg aaagccctgg ccaaggagct
     6121 gaacgtgccg atcctcgcct tgtcgcagct ttcgcgacag gtcgagcagc gcgacgacaa
     6181 gaggccgcaa ctgtcggatt tgcgcgaatc cggctcgatc gagcaggacg ccgacgtggt
     6241 tctgttcgtg ttccgcgagg aatattatct caagaaccgc gagccgcggc cggggacgga
     6301 ggagtacacc gcctggcaga acgagatgga gcgcgcccat ggccgcgccg aagtgatcat
     6361 aggcaagcag cgccacggcc cgaccggaac ggtcgagctg caattcgagg ccgagctgac
     6421 gcgtttcggc aatcttgcgc gcgacgagca aatgccggag cggtattgag agagcggcgc
     6481 gacgccgaag tcccctctcc ccgcggagcg gggagaggta cggagagggg cttggggagt
     6541 gaagctctaa gtttgaggct gaacgaccag cccctcaccg tactctcccc gtttcgctta
     6601 gctgcatggg gagaggggcc tggcggccgg gttagtctcg ctattccgag gttgctttta
     6661 gcgccgttat gctctctctc acgcctatga cctcgtcctc cgacgctggc ccggccttaa
     6721 ccgccgctgg cggcgttctc accatcgacc tttccgcgct cgccgacaat tggcgtcgtc
     6781 tcgccgagcg agtcgcgccg gccgaatgcg ccggcgtcat caaggcgaat gcctatggga
     6841 ccggtctgga gccggccgcg cgcgcgcttt tcgcggcggg gtgccgcacc tttttcgtgg
     6901 cgcaggtcgc cgagggcgtg cgcgcgcgcg ccgcgctcgg cgcggccgcg cgcattttcg
     6961 tgctcaacgg cctgcaggcc cgggccgagc ccgccgacta tgtcgcgcac cgcctggcgc
     7021 cgatcctcgg ctcgccggaa gagctcgcgc gctgggcgcc ctttgccgcg gacgggccgg
     7081 cttgcgcgat ccatctcgac accggcatga gccggctcgg cttcgcctcg ctcgacgcgc
     7141 tcgaagctgc gctcgccggc tatgaccaca gccggatcgc gctgatcatg agccatttcg
     7201 tttcctccga ggaacagggc gatccgctca acgatcgcca gatcgcgctg ttcgacaagg
     7261 cgcgggcgct gctgcccggc gttccggcca gcctcgccaa ttcatcgggg attttcctgg
     7321 cggggcggcc gttttacgat ctcgtccgtc ccggctacgc gctatacggc ggcaatccga
     7381 cgccggccac gcccaatccg atgcgttcgg tcgtcaggct ggaggcgcgc atccagcagg
     7441 tccggcaaat cgagcccggc gcgagcgtcg gctacaacgc gcaatggatg gccaagcggt
     7501 cgacgcgtct cgcgactctg ctgatcggct atgccgacgg cttgccgcgc agcgccggcg
     7561 cgacggatca gcggagcggc gccgacgtca taatcgcggg ccggcgctgt cctctggtcg
     7621 gccgcgtttc aatggacctt tgcgtcgccg acgtgacgga tattccggaa tccgcgccgc
     7681 ggccgggcga cagcgcggtc atcctcggcg aggagatcag cgtcgacgag cttggcgcgc
     7741 gcgccggcac gatcggctac cagatcctga ccagcctcgg cgcgaggtac cggcgggagt
     7801 acattccctt ctccccgcaa agcggggaga aggtggcgcg aagcgccgga tgaggggcag
     7861 cgccgagccc ggtgattggc gtttcgtaga gcgcgtacct ttcgagtccg gcgcagcccc
     7921 tcatccggcc gtgctgcgca cggccacctt ctccccgttt cacggggaga aggggattct
     7981 accgctcgac atccggcggc gaatccggct tccctggatc atggccaagg gcaaatcggt
     8041 tttcgtctgc cagaattgcg gcgcgatcac gcggcgctgg cagggcaaat gcgaatcctg
     8101 cggcgaatgg aacacgatcg tcgaggaggc cgagccttcc ggcatcggcg ccgcggcggc
     8161 gcggcgggcg gagaaaggcc gcgccttcgc gctcgagggg ctcgaaggcg gccccgcgca
     8221 agaggcgcgc acggtcatcg gcctcaacga gctcgatcgc gtcgccggcg gcgggctcgt
     8281 gcccggctcg gtggcgctga tcggcggcga gccgggcatc ggcaaatcga ccctgctgat
     8341 ccaggcctgc gccgctttgg cgcggcgcaa tggccgggtc gtctatattt ccggcgagga
     8401 atcgaccgcc caggtgcgtt tgcgagccgg ccggctcggc cttgccgacg ccaaagtgga
     8461 gctcgccgcc gagaccagcg tcgaggacat cgtcgcgacg ctttcgctcg gcgagccgcc
     8521 gagcctcgtc atcatcgact cgatccagac catgtggagc caggcggtcg aatcggcgcc
     8581 cggaaccgtt acgcaagtgc gcgcctcggc ccagagcctc atccgtttcg ccaaaagcag
     8641 cggcgccgcg gtgattctcg tcggccatgt caccaaggac ggccagatcg ccggcccgcg
     8701 cgtcgtcgag catatggtgg atgcggtcat gtcgttcgag ggcgaaggct cgcacgcctt
     8761 ccgcatcctg cgcgcgacca agaaccggtt cggcccgacc gacgagatcg gcgtcttcga
     8821 aatgaccggc ggcggcttga gcgaagtggt caatccttcc gctttgtttc tcgccggccg
     8881 cgacgcgtcg agccccggcg cagcggtctt tgccggcatt gaaggggcgc ggccgctgct
     8941 ggtcgaattc caggccctgg tcgcgccgac cacgctcggc acgccgcgcc gcgccgtggt
     9001 cggctgggat tcgagccggc tcgcgatgat tctcgccgtg ctggaagccc atggcggcct
     9061 gaagctcggc cagtacgacg tctatctcaa tgtcgccggc ggcctgaaaa tcattgagcc
     9121 cgcggccgac ctcgccgccg ccgccgcgtt ggcgtcttcg ctcgtcggcg ccagcctgcc
     9181 ggccgacgcg gtctatttcg gcgaaatcgg gctttccggg gcggtgcggc cggtggcgca
     9241 tgcggccttg cgcctcaagg aagcggccaa gctcggcttt cgccgcgccg tgacgccgcc
     9301 gccgcccaaa gaacgggacg agaccgtaaa gattgcgcgg accgaaattt cctcggtcgc
     9361 cagcctcatc gccgaaatcg cggcgatggg cgtgaaatcc gccaggtagc aaaaggatga
     9421 cgagatgagc gcgcttcgct ataaggcgcg gccaacgggg gcgccgcgtc ggccccgacc
     9481 tgctgcgcaa tccgcacgag gccgatgaaa tgccgtccta cctcgatctt ggcgtcattg
     9541 tgattgtcct gatctcggcc ttcctgtcga tggtgcgcgg cttcacccgc gaggtcttgg
     9601 cgatcggctc gtgggtggcg gcggccgccg cggcgtattt cttctatccc tacctgatgc
     9661 cctatgcgac gccctatatc cacaaagaga ttatcgctca ggcggtctgc gcggcggcgg
     9721 tattcttcgt gaccctggtc gtcgtctcga ccttcacggt gcgaatctcg gatgcgattc
     9781 tcgacagcaa gatcggcgcg ctcgaccgct ctctcggctt ccttttcggc gtcgcgcgcg
     9841 gctttctgct cgctgtcgtc gccttcgcga ttttcaattg gctggtcgcc gacaagcagc
     9901 agccgcaatg ggtcaaggac gccaagactc gcccgatcct caccgccacc gccgatcgcg
     9961 ttatcgccat gctgccggaa gacgctgagc acacgatcga gaattggctg aagagcggca
    10021 aagccggcgt tgcgcccgac caggcgccgc cgcccgacga gcagcagggg acgactccat
    10081 ccggcgcggc gccggcgact tcgcccgccg cgcccgcgcc tgcgccggcc gggtcgacgc
    10141 cgcaaaagaa gacggacgcc tcgccggacg ccagctccaa gcagaagctc gatgcgatca
    10201 tcaacggcaa gtccggagcg acgaagcaga actgatcggg cgcggacaat ttgacggagc
    10261 gagcggacgt taaggaggcg atgatttccg aagagccgtt tctcgatctc gacgcggatc
    10321 gcctgcgcga ggaatgcggc gtcttcggca tattcggcca ttccgacgcg gcggcgatca
    10381 ccgcgcttgg cctgcacgcc ctccagcacc gcggccagga ggcggcgggc atcgtcgcgt
    10441 ttgacggcgg ccgctttcat tccgagcgcc ggcttggcct ggtcggcgac catttctcca
    10501 aggcctcgac catcgagcgc ctgcccggct cggccgcggt cggccatgtc cgctattcga
    10561 cgaccggcga gacgattttg cgcaatgtgc agccgctttt cgccgagctc gacggcggcg
    10621 gcctggcggt ggcgcataac ggcaatctga ccaatgggct gaccctgcgc cgcgaattgg
    10681 ttcggcaagg ggcgatttgc caatcgacct cggataccga agcgatcctc catcttgtcg
    10741 cccgctcgcg acgacctacg atcatggagc gcttcatcga agcgttgcgg cagatcgaag
    10801 gcgcttatgc cctggtcgca ctgacaaaca agaagctgat cggcgcgcgc gaccccctcg
    10861 gcatacgccc gctcgtcctc ggcgagttcg agggctgtcc gatcctcgcc tcggaaacct
    10921 gcgcgctcga cattatcggc gcgaaattcg tgcgcgacgt cgagaatggc gaggtcgtcg
    10981 tcatctcgca gggcggcgtc gagtccttga ggccgttccc gccgcagcgc atgcgcccct
    11041 gcatcttcga atatatctat ttcgcccggc ccgactccat tgtgcacggt cgctcggtct
    11101 atgacgcgcg caaggccatg ggcgcggagc tggcgcgcga ggcgccggcg gacgcggacg
    11161 tcgtcgtgcc ggttcccg
//
LOCUS       DATGJW010000491        16637 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1133099, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000491 DATGJW010000000
VERSION     DATGJW010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 16637)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 16637)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..16637
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1133099"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1202
                     /locus_tag="VKU03_14700"
     CDS             <1..1202
                     /locus_tag="VKU03_14700"
                     /inference="COORDINATES: protein motif:HMM:NF012596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="enoyl-CoA hydratase/isomerase family protein"
                     /protein_id="HLJ72566.1"
                     /translation="AAVATIRGRARESRQRFLAAYAEKVYATLTHDFSRFVRVEELVL
                     DAADAFPGLTPSRAQLDGEADMLQRDKDGLEIDQGLLLSHILAHERSGRHLCHAMLLP
                     RPESAEALARFVADGALDLGRARLERKGRAAHLLAGNPRYLNAEDQTTIAAMEIAVDV
                     ATLDPASDIGVLRGAAVKHPKHAGRHIFGAGVNLTHIYRGKIPYVWFIQRDLGFVHKF
                     LRGVARPDSLPDDVHGFGVEKPWIAAIESFAIGGHCQTLLTMDYVLAASDAYLTLPAR
                     KEGIIPGASNLRLPRFVGDRIARQLIQYERRLECNSPEGRLICDEIAPPEEMDAAIDR
                     VVAGLASSGAVGAIGNRRAFRIGEEPLDLFRQYMAVYAREQAYCHFSPALIANLERYW
                     DAQNRKG"
     gene            1202..1888
                     /locus_tag="VKU03_14705"
     CDS             1202..1888
                     /locus_tag="VKU03_14705"
                     /EC_number="5.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008960781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047689 - aspartate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid racemase"
                     /protein_id="HLJ72567.1"
                     /translation="MPRRIGIVACSAEGAALCYRTICAEGAELFGPHAHPEIAIHSLS
                     LADYVACLENGDWRGVAELMLASAKKLATIGAEFLICPDNTIHQALPLVAPRSPLPWL
                     HIAETVAEEAVRRGFRKLAIVGTQWLVSGPVYPEKLAAHGLAYLMPNPTEREKINRII
                     MDELVYGVVKAEAAAYHARVFERMKREGCDAVVLGCTEIPLIVNDENSPLPTLDSTRL
                     LARAALREAA"
     gene            2008..2796
                     /locus_tag="VKU03_14710"
     CDS             2008..2796
                     /locus_tag="VKU03_14710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018245069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HLJ72568.1"
                     /translation="MDVIRRNNVHVAGRGVRPMVFAHGFGCDQNMWRYVAPAFERDFK
                     TVLFDHVGAGGSDLAAYSREKYSSLQGYADDVVEICRALALTDIVFVGHSVSSVIGIL
                     AAASAPDLFEALVLIGPSPRYVNDGAYVGGFTESQIEELLEFLDSNHMGWSAAMAPTI
                     MGNPDRPELAEELTNSFCRTDPEIAKHFARTTFLSDNRADLARVRARCLVLQCSEDII
                     APNCVGEYVHAHLPNSRLVVMKATGHCPNLSAPAETIDAIRAFV"
     gene            2824..4473
                     /locus_tag="VKU03_14715"
     CDS             2824..4473
                     /locus_tag="VKU03_14715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018116032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ72569.1"
                     /translation="MSVDLEDLFENAPCGYLSTGPGGRIERANATFLRWMGYVQGELA
                     GKPFQELLNIAGRIYYETHFAPLLRIQGFFNEVALDLVKRDGKRLPTLVNAVERRGAA
                     GELLFTRITIFNASDRRRYESELLEARKAAEQANAALRELNATLEARVASELEKRMNA
                     EEAFRQAQKMEAIGQLTGGIAHDFNNLLTIVIGGLDMIDRQLQKMPESPEAARIRRSR
                     AMALDGAKRAATLTSRLLAFSRRQPLAPRPLDLNRLVTGLGDMLQRSLGETIALETVS
                     GAGLWPAFADPSQLESAIVNLAVNARDAMPRGGKLTIETANAYLDQAYASTAPEPVKP
                     GQYVQIAISDTGVGMTPEVLAKAFDPFFTTKEVGKGTGLGLSQVHGFVRQSGGHIRIY
                     SEPGLGATVKLYLPRADGGVASKEGYEPTARTLAGGRETILVVEDDDMLREFSGSALS
                     ELGYRVLYAKSAPSALELLQSERVDLLFTDVVLPDGANGRQLAEEAERRRPGLKVLYT
                     TGYTRNAIIHCGRLDAGVSLIGKPFTFEELARSVRSILDRN"
     gene            complement(4498..5286)
                     /locus_tag="VKU03_14720"
     CDS             complement(4498..5286)
                     /locus_tag="VKU03_14720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003537240.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YafY family protein"
                     /protein_id="HLJ72570.1"
                     /translation="MRKASRLFEIIQILRLAKRPITAAAIAERLEVAPRSIYRDIAAL
                     QAMRVPIDGGRGVGYILRPGFELPPLMFSIEETEATVLALALLERTGDAELKQAAKRV
                     NQKIAAAVPPPLRGAFDARSLHAWGAIAPAPQGVDLGAVRRAIRDERKLAIAYRDEMG
                     RSTKRTIWPLALIYYSDVANIVAWCELRQALRNFRTDRVQQSAPTDDFFHGAGDRLRA
                     LWIAGWRINDEERPAGRRSAERQDRQPASQPVIRLAPGASRSRS"
     gene            5408..6049
                     /locus_tag="VKU03_14725"
     CDS             5408..6049
                     /locus_tag="VKU03_14725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010968961.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HLJ72571.1"
                     /translation="MSYTENLWLFLLLLFGIIVVPGMDMMFVLANALTGGRRAGLSAT
                     AGVMLGGAAHTLIGATGVGVLIKLAPSLYIGMLIAGAAYMAWIGASLARSSIIVGKVA
                     GAASRSLWTAFRRGTATCLLNPKAYLFVLSVYPQFLKPQYGALWLQALVMGAMTLLIQ
                     LCVYGGLALAAGRSRDFLVSNPRTTMLVGRAAGALLILIAALTAWHGWAGAAR"
     gene            6182..7180
                     /locus_tag="VKU03_14730"
     CDS             6182..7180
                     /locus_tag="VKU03_14730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018407525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ72572.1"
                     /translation="MTIALSFMAGLAAFLEAGPAFALDKVTFGANWLADPEAGGFYQA
                     AVDGTYAKYGLDVTILPGGPQANGGMLLLAGKVDFFMGGDMLGGFLAVARNIPIVAVA
                     AEFQKDPQIFMSHPGVGLDKWTDLPKATAYVSAGGVYSFYAWMIKAWGFKAGNVRPYD
                     FNSAPFIADKHSIQEGYVTSEPYAVEKQGGFKPNIFLLADYGYSTYATLIEATRATVE
                     KKPDLVQRFVDASAIGWYHYLYGVNRKANEAIKKANPDMTDGQIAYSIAALKKYGIVD
                     SGDALTLGIGAMTDARMKAFFDEMAKVGLLKPDLDYKKAYTLRFVDKGVGVGLRPK"
     gene            complement(7200..7766)
                     /locus_tag="VKU03_14735"
     CDS             complement(7200..7766)
                     /locus_tag="VKU03_14735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004519 - endonuclease activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Uma2 family endonuclease"
                     /protein_id="HLJ72573.1"
                     /translation="MANASKQLPDRRFTVAEVETMVAAGVMEEDERVELIHGELVPMS
                     PKGTQHEVLKVALLDRWYRLRPGDCALAPETTFRLSEDTYLEPDIVIYRRADGLKKLS
                     GTGVLLVVEIADSSLRYDMGRKARLYASFGVRELWVIDAVKLAVRMFRAPSTEGFRES
                     RDFGASERLVPLFAPAAFALRLDELQFD"
     gene            complement(7894..8583)
                     /locus_tag="VKU03_14740"
     CDS             complement(7894..8583)
                     /locus_tag="VKU03_14740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007597325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ72574.1"
                     /translation="MTASYGQFCPVAMAAEIFCTRWTALVMRELLCGSQRFNDIRRGV
                     PRMSATLLSKRLKELEQAGLVVVRQGAHGGSEYRLTAAGEDMRDLVMTLGFWGQRWIE
                     ARQSLKNLDPSLLMWDMRRHLDPTPIPPRRCTIRFHYPELPPAQRDWWLVVDGRAVDL
                     CRIDPGFDVDLHVETSLRTMTSIWMGLATLAEETAEGRIALSGDPAISRSMQQWLGLS
                     PFAKERPRKVA"
     gene            8779..9924
                     /locus_tag="VKU03_14745"
     CDS             8779..9924
                     /locus_tag="VKU03_14745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011581425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ72575.1"
                     /translation="MSAAETLAIASDPLSIARWLGPIFAERAEKAGDSDEFVAANFSD
                     LKASGLVEAGVPAELGGGGADVVALSAMLRELARHCGSTALAFSMHTHQVAIPAWRWT
                     RQKATAVAPLLKKVAQERLVLLSSGGSDWIAGSGKAERVEGGYRITARKIFCSASPAG
                     DMLMTAAVLDGEPEGPNVLHFAVPMHAPGVTILDNWRAMGMRGTASNDVLIEGYFAPE
                     ASIGATRKQGRWHPLFEITAMIAFPLIYAAYLGVAEGARTIAVKMAAKRRASRHVVEQ
                     VGRMETELRAAQYAHEAMVAAAARDEPGPATTNEIMIGRALVARHALAATELAIEAAG
                     GAAFYREHGLERRFRDIQGARYHPMQTGPQQEFAGRMALGLDVSTTF"
     gene            complement(9965..10975)
                     /locus_tag="VKU03_14750"
     CDS             complement(9965..10975)
                     /locus_tag="VKU03_14750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72576.1"
                     /translation="MEIEAPGRTDLVGLLARFAPLVFLVVLMIAFSLFQPRFLSPFNL
                     FNVMRQVSVYGLLAVGMTFVILTAGIDLSIGSLLAFAGLVAAAVDKGGISNRFSVGAG
                     QDALGYGWPLAALAAVAVGLAGGWLQGQAITKFKVPPFVVTLGGMSVFRGAALLFAGG
                     GPISGFDPNYVWWGQGKIGPVPVPVIIFVAFAVLAHIALRYARYGRQVYAVGGNPEAA
                     RLAGLNVLRVIGSVYVIMGFFAGLGGFVLSARLNSSEAVAGVGYELTVIASVVIGGTS
                     LFGGVGTIFGTVIGTILIGVLLNGLVLMEVSPYVQQIIIGVIIVLAVAFDTFAKSRKR
                     TA"
     gene            complement(11007..12536)
                     /locus_tag="VKU03_14755"
     CDS             complement(11007..12536)
                     /locus_tag="VKU03_14755"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ72577.1"
                     /translation="MTEPLLRMSGISKRFPGVQALDDVGLEVSPGEVHALLGENGAGK
                     STLLKILSGAQQPDAGSISLAGAPVMLASPQDAQRLGIVTIYQEFTLAPNMSVAENVF
                     IGREPGAGPFVNWRKMAAETRAITERLGLGLKPRALVRDLSVAEQQMVEIARALSMQS
                     RLIVMDEPTSALSSAEVEKLFRIIRDLKAQGLSIIFVTHRLEEMMEICDRYTVLRDGR
                     RVGAGKVSDTDIDGIIRLMVGRQVDALFAHRAPAKTGAVALKVEGLSRRGKAQDQNAT
                     VLDNVSLEVRRGEILGLAGLVGAGRTELARAIFGADPFDSGRVLIDGEPAAIRSPQDA
                     IRRGVGLVPEDRKQQALFLSLGVRFNLSMAAHEHILRGKFFIDEAAERRMVEEYRKAL
                     NIRMASQEQIIANLSGGNQQKVVLARWLALQPKVLIVDEPTRGIDIGAKVEVHNLLFE
                     MAQRGIAVIAISSELAEILAISDRIVTMREGRITGEIDRENANEERLMTLMTTLGAKA
                     A"
     gene            complement(12655..13623)
                     /locus_tag="VKU03_14760"
     CDS             complement(12655..13623)
                     /locus_tag="VKU03_14760"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006336774.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ72578.1"
                     /translation="MIFRNVAKAALVAGVASLALTFGAPAQAAGKHLTILVTFPDMAF
                     PFFTHMMAEVKAEAAKIGDIDIIESDGQRSSPKQTADIEAAITKGVNGMFLDPNEVDA
                     LAPAVQEAVDAKIPVVTVDRRVDKVPGVLAHVGADNVKGGEAQGELIEKLFPNGAKVM
                     NLQGQSGSSPAIDRNKGLHNVLDKAKDKYKFVFEDTAGFDRAKGLSMSETALDGMTSP
                     PDVIVCANDDMALGALEALKARNLIGKVKLIGFDALPEALGQIKAGNMTATIEQSPGG
                     QSQKALDILVAFLRDGKQPAEKVTLLTPVAITKDNLDKAERLSEVK"
     gene            13885..14721
                     /locus_tag="VKU03_14765"
     CDS             13885..14721
                     /locus_tag="VKU03_14765"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLJ72579.1"
                     /translation="MDYATRLARPFSGAAAERQARVSRRLAPSRFLAGPFQRLGYDSS
                     EAWAWENYRSVVLGLAQASRRRDGEPTRMLEIGGGRGPLLTPAEASAAGIAVTVNDVD
                     AQELAMGPQEFEKAQFDIAGEIDPALHGKFDLVVSRMVMEHVGDGRKAWANMAALLAP
                     GGVALSFHPTLYAPPYLINLAFPEALTARVLRFFFPNRHQGDYPKFPARYDLCFSDPA
                     KVEPVLKACGFSEVLIAPFWGHGYFRHIPLLRELDAALQRVAEAREWRGLTTYAFALA
                     RR"
     gene            complement(14758..16050)
                     /locus_tag="VKU03_14770"
     CDS             complement(14758..16050)
                     /locus_tag="VKU03_14770"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HLJ72580.1"
                     /translation="MTAAETVRTRPRTASLSIGKLQRALLWLFVASGALASIEPSPYE
                     LMFIPALLAFGWRDLLFDRAMAPLIVGLALFNVGGALSLLPYIDQRESYMFSAISLYI
                     SVTAVFFAALVAKAPLERLAVIRSGYVVAGMIAGLAGILGYFNVAGLAEHFTLYNRAS
                     GTFKDPNVLGPFLAPPLIWLFQAILLRQAKGFFRTYLPLLVMLTALFLSFSRGAWGVW
                     IASTLMMVGLTYLTTPSSALRRRIVILSILGAAILVVLLAIALSIPDIRSVFEIRFSL
                     SQDYDVGQTGRFGDQWRSIPMLLDRPLGFGPLQYRNHFEGVDPHNVYVNAFASYGWIG
                     GLCYAALIAATVALGWRLVFQRTPYQTEAIAIWSCLFVQILQGFQIDTDHWRHLFLLL
                     GALYGLLAATRAHNRRSLVADSVIHAKAGIRRAAAHEG"
     gene            complement(16180..>16637)
                     /locus_tag="VKU03_14775"
     CDS             complement(16180..>16637)
                     /locus_tag="VKU03_14775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018260600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HLJ72581.1"
                     /translation="NEVVEVFKFRSMRTDMLDFDASKLVTKEDPRVTRVGRFIRKTSL
                     DELPQLFNVVFKGNLSLVGPRPHALHAKAAERQYDEVVDGYFARHRVKPGITGWAQIN
                     GWRGETDTPEKIQHRVEHDLHYIENWSILLDLYILAITPYSLIKTKNAY"
BASE COUNT         2937 a         5368 c         5429 g         2903 t
ORIGIN      
        1 cggccgcagt cgcgacgatt cgcggccgcg cccgcgaatc gcgccagcgc ttcctcgccg
       61 cttacgctga gaaagtctat gcgaccctga cgcatgactt ttcgcgcttc gttcgcgtcg
      121 aggagcttgt cctcgacgcc gccgacgctt ttccgggcct gacgccgtcg cgcgcgcagc
      181 ttgacggcga agccgatatg ctccagcgcg acaaggatgg gttggagatc gaccaggggc
      241 tgctcctgtc gcacattctc gcccacgagc gctcgggacg ccatctctgc cacgccatgc
      301 tgctgccgcg gccggaaagc gccgaggcgc tggcgcgctt cgtcgccgac ggcgcgctcg
      361 atctcggacg agcgcggctc gagcgcaagg gcagggcggc gcatcttctc gccggcaatc
      421 cgcgctatct caacgccgaa gaccagacga cgatcgcggc gatggagatc gcggtcgatg
      481 tcgcgacgct cgatccggca agcgacatcg gcgtgctgcg cggcgccgcg gtcaagcatc
      541 ccaaacatgc cggccggcat attttcggcg ccggcgtcaa tctcacccat atctatcgcg
      601 gcaagattcc ctatgtctgg ttcatccagc gcgatctcgg cttcgtgcat aaattcctgc
      661 gcggcgtggc gcggccggat tccttgcctg acgacgttca tggcttcggc gtcgagaagc
      721 cgtggatcgc ggcgatcgag agtttcgcga tcggcggcca ttgtcagacc ctgctgacca
      781 tggattacgt ccttgccgcg agcgacgcct atctcaccct tccggcgcgc aaggaaggga
      841 tcattcccgg cgcctccaat ctccgcctgc cgcgcttcgt cggcgaccgc atcgcccgcc
      901 aactcatcca gtatgagcgc cggcttgaat gcaattctcc ggagggccgc ttgatctgcg
      961 acgagatcgc gccgcccgag gagatggatg cggcgatcga cagggtcgtc gccggtctcg
     1021 ccagttccgg cgcggtcggc gcgatcggca atcgccgcgc tttccgcatc ggcgaggagc
     1081 cgctcgatct cttccgccag tacatggcgg tctatgcgcg cgagcaggct tattgccatt
     1141 tcagcccggc tttgatcgcc aatctggagc gctattggga cgcgcagaac aggaagggct
     1201 gatgccgcgc cgcatcggaa tcgtcgcctg ctcggcggag ggcgcggcgc tatgctatcg
     1261 cacgatctgc gcggaaggcg ccgagctttt cggaccgcac gcccatccgg aaatcgcgat
     1321 ccattcgcta tctttggccg actatgtcgc ctgcctggaa aacggcgatt ggcgcggcgt
     1381 cgccgaattg atgcttgcct cggcgaaaaa actggcgacg atcggcgccg agttcctgat
     1441 ctgtcccgac aacacgatcc atcaggcttt gccgctcgtc gcgccgcgct cgccgctgcc
     1501 ttggctgcac atcgccgaaa ccgtcgccga ggaggcggtc aggcgcggtt tccgcaagct
     1561 cgcgatcgtc ggcacgcaat ggctcgtttc cggcccggtc tatcctgaga aactcgccgc
     1621 gcacggcctt gcctatctca tgccgaatcc gaccgaacgc gaaaagatca accgcatcat
     1681 catggacgag ctggtttatg gcgtcgtgaa ggcggaggcg gccgcctatc acgcccgcgt
     1741 cttcgagcgg atgaagcgcg agggttgcga cgccgtcgtc ctcggctgca cggaaattcc
     1801 actgatcgtg aacgacgaaa attcgccgct tccgacgctc gattcgacgc gccttctcgc
     1861 ccgcgcggcg ctgcgcgagg cggcgtagaa aaccgtccgc cgcggtcccc cgatatggga
     1921 cgcaaaacaa gcggttccgc cggcgttgga accgaacgcc ccgctgcggc gtttcttggc
     1981 gtttgcggag ttggagcgga ggggcgcgtg gacgtgattc ggcgcaacaa tgtccatgtc
     2041 gcggggcgcg gcgttcggcc aatggtgttc gcgcatggct tcggctgcga ccaaaacatg
     2101 tggcgctacg tggccccggc tttcgaacgc gatttcaaga ccgtgctctt cgatcatgtc
     2161 ggcgcgggcg ggtcggatct tgccgcctat tcgcgcgaga aatattcgtc tctgcagggc
     2221 tatgccgacg atgtggtcga gatttgccgc gcgcttgcct tgaccgacat tgtctttgtc
     2281 ggccactcgg tcagcagcgt gatcggcatc ctcgcggcgg ccagcgcgcc cgatctgttc
     2341 gaagcgctcg ttcttatcgg cccgtcgccg cgatacgtta atgacggcgc ctatgtcggc
     2401 ggtttcaccg aatctcaaat cgaagaattg ctggaattcc tcgacagcaa ccatatggga
     2461 tggtcggcgg caatggcgcc gaccattatg ggcaatccgg accggcctga actcgccgag
     2521 gaactgacga acagtttctg ccggaccgac ccggaaatcg ccaagcattt cgcgcgaaca
     2581 acgttcctgt ccgacaatcg cgccgacctt gcccgcgtcc gcgcgcgatg cctggtcttg
     2641 cagtgcagcg aggacattat cgcgccgaac tgcgtcggcg aatatgtcca tgcccatttg
     2701 ccgaacagca ggctggtggt gatgaaggcg accgggcatt gcccgaattt aagcgcgccg
     2761 gcagagacga tcgacgcgat ccgcgccttt gtctgatccg gcgtcgcatt gcgaagcggc
     2821 cgcatgagcg tcgatcttga agatctcttc gaaaacgcgc cctgcggcta cctgtccacc
     2881 ggtccgggcg gccggatcga gcgcgccaat gcaacgttcc tgcgctggat gggctacgtc
     2941 cagggcgagc ttgccggcaa gcctttccag gagcttctca acattgccgg gcggatctac
     3001 tacgagacgc attttgcgcc gctcttgcgc atccagggct tcttcaacga ggtcgcgctc
     3061 gatctcgtga aacgcgacgg caagaggctg ccgacgcttg tcaacgccgt cgaacggcga
     3121 ggcgcggccg gcgaattgct gttcacccgc atcacaatct tcaacgccag cgaccggcgg
     3181 cgctacgaaa gcgaactcct ggaggcgcgc aaggcggcgg agcaagcgaa cgccgccttg
     3241 cgcgagctca atgcgacgct tgaagcgcgc gtcgcatccg agctggagaa gcggatgaac
     3301 gcggaggagg ctttccggca ggcgcagaaa atggaggcga tcggccagct caccggcggg
     3361 atcgcgcatg atttcaataa tcttctgacg atcgtcattg gcggcctcga tatgattgat
     3421 cgacagctcc agaagatgcc ggaatcgccc gaagcggcgc ggattcgccg ctcgcgcgcc
     3481 atggcgcttg acggggccaa gcgggcggcg acgctgacgt cgaggctgct cgccttctcg
     3541 cggcgccagc cgctcgcgcc gaggccgctc gacctcaatc gcctcgtgac cgggctaggc
     3601 gacatgctgc agcgctcgct tggcgagacg atcgcgctcg agacggtgtc gggcgccggg
     3661 ttgtggccgg ctttcgccga tccctcgcag ctcgaaagcg cgatcgtcaa tcttgcggtg
     3721 aacgccaggg acgcgatgcc gcgcggcggc aagctcacca tcgaaacggc caacgcctat
     3781 ctcgatcagg cctatgcttc gacggcgccg gagccggtca agccgggaca atatgtccag
     3841 atcgccatat ccgacacggg cgtcgggatg acgccggagg tcctggcgaa agccttcgat
     3901 ccgttcttca ccacgaaaga ggtcggcaag gggaccggcc ttggcctcag ccaagtacat
     3961 ggctttgtgc gtcaaagcgg cggccacatt cggatctaca gcgagccggg gctgggcgcg
     4021 acggtcaagc tctatttgcc gagagcggac ggcggggtcg cctcgaagga gggttatgag
     4081 ccgaccgctc gcacgcttgc cggcggcagg gagacgatcc ttgtcgtcga agacgacgac
     4141 atgctgcgcg agttcagcgg gagcgcgctg agcgagctcg gctatcgcgt tctctatgcg
     4201 aagtctgcgc cgagcgcgct cgaattgctg caaagcgaac gcgtcgatct gctcttcacc
     4261 gatgtggtgc tgccggacgg agcgaatggg cggcaactcg ccgaggaagc cgagcgccgc
     4321 cggcccggtc tgaaagtgct ctacacaacc ggctatacgc gcaacgctat catccattgc
     4381 ggtcggctcg acgccggcgt cagcctgatc ggcaagccgt tcacgttcga ggagctcgcc
     4441 cggagcgtac gctccatcct cgaccgaaac tagaaggcgc aaattcttcg cgaagtttca
     4501 ggatcgagat cgagacgctc ccggggcgag gcggatgacc ggctgcgagg ccggttgacg
     4561 atcctgcctt tcggcgcttc gccgtcccgc gggccgctct tcgtcattga ttctccatcc
     4621 cgcgatccac aacgcccgca gccggtcgcc cgcgccgtgg aagaaatcgt cggtaggcgc
     4681 gctttgctgc acgcggtcag tccggaaatt gcgcaacgct tggcgcagct cgcaccaagc
     4741 gacgatgttc gcgacatcgg aatagtagat cagcgccagc ggccagatcg tccgcttggt
     4801 cgagcgtccc atctcgtcgc gataggcgat cgcgagcttg cgctcgtcgc gaatggcgcg
     4861 gcggacggcg ccaaggtcga cgccttgcgg agcaggggcg atcgcgcccc aggcgtggag
     4921 cgatctggcg tcgaaggcgc cgcgcaaagg cggcgggacg gcggcggcga ttttctggtt
     4981 gacgcgcttg gccgcctgct tgagctcggc gtcgccggta cgctccaaca gcgcgagcgc
     5041 cagcacggtc gcctcggtct cctcgatcga gaacatcaga ggcggcagct cgaatcccgg
     5101 ccgcaggata tagccgacgc cgcggccgcc gtcgatcggc acccgcatcg cctgcaacgc
     5161 cgcgatgtcg cggtagatcg agcgcggcgc gacttccagc ctctcggcga tcgccgcggc
     5221 ggtgatcggc cgcttagcga gcctcaaaat ctggatgatc tcgaacaggc gggacgcctt
     5281 gcgcatgccg aagctcccgg ccttcgccct cttcacgcaa ctgacagaag gctgtcagtt
     5341 ggcctcgtgt acatcgctcc gccccgattt ggaagacggg gcctcaaagg cgccaaggcg
     5401 gacgcgaatg tcgtacacgg aaaatctctg gctattcctg cttctcctgt tcggcatcat
     5461 cgtcgtgccg ggcatggaca tgatgttcgt gctcgccaac gccttgaccg gcgggcgccg
     5521 cgccggcctt tcggcgacgg cgggcgtcat gctgggcggg gcggcgcata cgctgatcgg
     5581 cgcgaccggg gtcggcgttc tcatcaagct ggcgccgtcg ctctatatcg ggatgctcat
     5641 cgctggcgcc gcctacatgg cctggatcgg cgccagcctg gcgcgaagct cgatcattgt
     5701 cggcaaagtc gccggcgccg caagccgctc cttgtggacg gcgttccggc gagggacggc
     5761 gacctgcctg ctcaatccca aggcttatct cttcgtgctt tccgtctatc cgcaattctt
     5821 gaagccgcaa tatggcgcgc tctggctgca ggcgctggtc atgggggcaa tgacccttct
     5881 gatacagctt tgcgtctatg gcggcctggc gctcgccgcc ggccggagcc gcgattttct
     5941 cgtctccaat ccgcggacga cgatgctggt cggccgcgcc gccggcgctc tgctcatcct
     6001 gatcgcggcc ctgacggcct ggcatggctg ggcgggcgca gcgcggtagc ggctttcaaa
     6061 cagcgccgat gaggcgtcgg cttggatcgg aaatcgcctc cgggcgttct tacccggaac
     6121 gaccctggat gtagctctct tgaacgcgca atcaggtaaa tcgagccggc tcgcgggccc
     6181 tatgacgatc gccctttcct ttatggcggg gcttgccgct ttcctggagg ccggacccgc
     6241 cttcgcgctc gacaaggtga cgttcggcgc caactggctc gccgatccgg aggccggcgg
     6301 cttctatcag gcggccgtcg acggcactta cgcgaaatac gggctcgacg taaccatcct
     6361 gcccggcggc ccgcaggcca atggcggcat gctgcttctc gccggcaagg tcgacttctt
     6421 catgggcggc gacatgctcg gcggctttct cgcggtggcg cggaacattc cgatcgtggc
     6481 ggtcgccgcc gaatttcaga aggacccgca aatcttcatg tcgcatccgg gcgtcggcct
     6541 ggacaagtgg accgatctgc ccaaggcgac cgcctatgtc agcgcgggcg gcgtctattc
     6601 tttctatgca tggatgatca aggcctgggg attcaaggcg gggaatgtcc ggccctacga
     6661 tttcaattcg gcgccgttca tcgccgacaa acattcgatc caggagggct acgtcacgtc
     6721 cgagccttac gccgtggaga agcaaggcgg cttcaagccg aatattttcc tgctcgccga
     6781 ttacggctac tcgacttatg cgaccttgat tgaagcgacg cgcgcgaccg tcgagaagaa
     6841 gcccgatctc gtgcagcgct tcgtcgacgc ctcggcgatc ggctggtatc actatctcta
     6901 cggcgtcaat cgcaaggcca acgaggcgat caagaaggcc aatcccgaca tgaccgacgg
     6961 ccagatcgcc tattcgatcg ccgcgctcaa gaaatacggg atcgtcgatt caggcgacgc
     7021 gctgacgctc ggcattggcg cgatgaccga tgcgcggatg aaggcgttct tcgatgaaat
     7081 ggccaaggtc ggccttttga agccggacct cgattacaag aaggcttata cgctgcgctt
     7141 cgtcgacaag ggagtcggcg tcggattgcg gccgaaatag agcccgcgag cggcgacgct
     7201 cagtcgaatt ggagttcgtc gaggcgcagc gcaaaggcgg cgggcgcaaa caacgggacc
     7261 aatcgctccg acgctccgaa atcgcggctt tcgcggaagc cttcggtcga aggcgcgcgg
     7321 aacattcgaa cggcgagctt gacggcgtcg atgacccata gctcgcgcac gccgaacgat
     7381 gcgtagagcc tcgccttgcg acccatatcg tagcgtaaag aggaatcggc gatttctacg
     7441 acgaggagca cgcctgttcc ggaaagcttc ttcaggccat cggcgcgtcg atagatgaca
     7501 atgtcgggct cgaggtaagt atcctcggag aggcggaagg tcgtctccgg cgccaaagcg
     7561 caatcgccag gccttaggcg ataccagcga tcgagcaggg cgaccttgag gacttcgtgc
     7621 tgggttccct tgggcgacat cggaaccagc tccccatgga tcagctcaac ccgctcgtct
     7681 tcctccataa cgccggcggc gaccatcgtc tccacctcgg cgaccgtgaa tcggcggtca
     7741 ggcaattgtt tggacgcgtt cgccatgatc tgtcctcaaa ctctcttgga gcgcccgctg
     7801 taagccgagc ctatcacgaa agctcgccgg ccggcgaggc gagcgttcct gagcgctatt
     7861 cgtgggcggc gcggcctgcg cctcatggct aaactatgcg acctttcgcg gccgctcctt
     7921 ggcgaagggg ctgaggccca gccattgctg catcgagcgc gaaatggcgg gatcgccgct
     7981 cagcgcgatc cgtccttcgg ccgtttcttc ggcgagggtg gcgaggccca tccagatcga
     8041 cgtcatggtc cgcagcgacg tctcgacatg caggtccacg tcgaagcccg gatcgatccg
     8101 gcagagatcg accgcccgcc cgtcgacgac gagccaccaa tcgcgttgcg cgggcggcag
     8161 ctccggatag tgaaaccgta tcgtgcagcg gcggggcgga atcggcgtcg gatcgagatg
     8221 gcggcgcatg tcccacatca gcagcgaggg atcgagattc ttcagcgatt gacgggcttc
     8281 gatccagcgc tggccccaga agccgagcgt catgacgaga tcgcgcatat cctcgccggc
     8341 ggcggtgagc cgatattccg agccgccatg cgcgccctgc cggacgacga ccaggccggc
     8401 ttgctcgagc tctttgagcc gtttcgacag cagggtcgcc gacatccgcg gcacgccgcg
     8461 gcgtatgtcg ttgaagcgct ggctgccgca gagaagctcg cgcatgacga gagcggtcca
     8521 ccgcgtgcag aagatttccg cggccatcgc caccggacag aactggccat agctggcggt
     8581 catctcgcgg cttctccctc tcgccggcag ggcgagagtt taagccagga ccgccgtcgc
     8641 gacgagtgca gaatctggac cagaaaatcg tcgcgaacgc ttgccggcgc gcgcgccgct
     8701 ccatttccag gactggacgg gacggagccc tcctggcgag attcgcgcca tgccgcgtct
     8761 ggcgagtgga gggacctcat gagcgccgca gaaaccttgg cgatagcaag cgatccattg
     8821 tcgatcgccc ggtggcttgg cccaatcttc gcagagcggg cggaaaaagc gggggattcg
     8881 gatgaattcg tcgccgcaaa tttcagcgat ctcaaggcgt ccggcctggt cgaggccggc
     8941 gtgccggcgg agctcggcgg cggcggcgcg gatgtcgtgg cgctaagcgc gatgctccgc
     9001 gaattggccc gccattgcgg ttcgacggcg ctcgccttct ccatgcacac ccatcaggtc
     9061 gccattccgg cctggcgctg gacccgtcag aaggcgaccg cggtcgcccc gctcctcaaa
     9121 aaggtcgccc aggagcggct ggtcctgttg tccagcggcg gctcggactg gatcgcgggc
     9181 tccggcaaag ccgagcgggt cgaaggcggc tatcgcatca ccgcgcgcaa aatcttctgc
     9241 tcggcctcgc cggccggcga catgctgatg accgccgcag tgctggacgg cgagcccgaa
     9301 ggcccgaacg tgctgcattt cgccgtgccc atgcatgcgc ctggcgtgac gatcttggac
     9361 aattggcggg cgatgggcat gcgcggcacg gcctcgaacg acgtgctgat cgaaggctat
     9421 ttcgcgcccg aggcctcgat cggcgcgacg cgcaagcagg ggcgatggca tccgctgttc
     9481 gagatcaccg cgatgatcgc cttcccgctg atctacgccg cctatctcgg cgtcgccgaa
     9541 ggcgcccgga cgatcgccgt caaaatggcg gcgaaacgcc gggcgagccg tcatgtggtc
     9601 gagcaggtcg gccgcatgga gaccgagctc cgcgccgcgc aatacgcgca cgaggcgatg
     9661 gtggccgctg cggctcgtga cgagccggga ccggcgacga cgaacgagat catgatcggg
     9721 cgcgcgctcg tcgcaaggca tgccttggcg gcgaccgagc tggcgatcga ggccgcgggc
     9781 ggcgccgcct tctaccgcga gcacggcctg gagcgccggt ttcgcgatat tcaaggcgcc
     9841 aggtaccatc cgatgcagac gggcccgcag caggaattcg ccggccgaat ggcgctcggg
     9901 ctcgacgtca gtacgacgtt ctgaagatcg cccgtttccg ccgcgccgaa accggcggcg
     9961 gccttcacgc cgtccgcttg cgcgattttg cgaacgtatc gaacgcgacg gcgagaacga
    10021 tgatcacgcc gatgatgatc tgctggacgt agggcgagac ttccatcagg acgaggccgt
    10081 tgagcagcac gccgatcagg atcgtgccga tcaccgtgcc gaatatcgtg ccgacgccgc
    10141 cgaacaggct ggtgccgccg atcaccaccg aggcgatcac ggtcagctca tagccgacgc
    10201 cggccaccgc ctccgacgag ttcagccgcg cggaaagcac gaagccgccg agcccggcga
    10261 agaagcccat aatcacatag acgctgccga tcacccgcaa aacgttgagg ccggcaaggc
    10321 gcgccgcctc gggattgccg ccgacggcat agacctgccg gccatagcgg gcgtagcgca
    10381 gcgcgatgtg agcgagcacg gcgaaggcga cgaagatgat caccggcacc ggcaccggcc
    10441 cgatcttgcc ctggccccac cagacataat tgggatcaaa accgctgatc ggtccgccgc
    10501 cggcgaacag caaggcggcg ccgcgaaaca ccgacatgcc gccgagcgtc acgacgaagg
    10561 gcggcacttt gaacttcgtg atcgcctgtc cctgcagcca gccgccggcg aggccgaccg
    10621 ccaccgcggc caaagccgcg agcggccagc catagccgag cgcgtcctgg ccggcgccga
    10681 ccgagaagcg gttggagatg ccgcctttgt cgacggcggc cgcgaccagg ccggcgaagg
    10741 cgagcagcga cccgatcgaa agatcgatcc cggcggtcag gatcacgaaa gtcatgccga
    10801 cggcgagcag gccatagacc gagacctggc gcatgacatt gaacagattg aacgggctca
    10861 ggaagcgcgg ctgaaagagg gagaaggcga tcatcagcac gacgagaaag accagcggcg
    10921 cgaagcgggc gagcaggccg acgagatcgg tccggcccgg cgcctcgatc tccatgcgtc
    10981 ttgcttcggt cacgcccgtc tccagttcac gccgctttgg cgccgagggt ggtcatcaaa
    11041 gtcatcagcc gctcttcatt ggcgttttcg cgatcgatct cgccggtgat ccgcccctcg
    11101 cgcatcgtca cgatccgatc gctgatcgcc agaatctcgg cgagctccga cgagatggcg
    11161 atgacggcga tgcccctctg cgccatttcg aagaggagat tgtgcacctc gaccttcgcg
    11221 ccgatatcga tgccgcgcgt cggctcgtcg acgatcagca ctttgggctg cagggcgagc
    11281 cagcgggcga gcaccacttt ttgctgattg ccgccggaaa gattggcgat gatctgctcc
    11341 tggctcgcca tgcggatatt gagggccttt cggtattcct cgaccatgcg ccgctcggcc
    11401 gcttcgtcga tgaagaactt gccgcgcaga atgtgctcat gcgcggccat gctcagattg
    11461 aaccggacgc cgagcgacag gaaaagcgcc tgctgcttgc ggtcctcggg cacgaggccg
    11521 acgccgcggc ggattgcgtc ctgcggcgag cggatcgccg ccggctctcc gtcgatcaaa
    11581 acgcggcccg aatcgaacgg atcggcgccg aagatggcgc gcgccagttc tgtccggccg
    11641 gcgccaacca agccggccaa gccgaggatt tcgccgcgcc gcacctcgag cgagacattg
    11701 tcgagaaccg tcgcgttctg gtcctgcgcc ttgccgcgcc gcgacaggcc ttccactttg
    11761 agcgcgacgg cgcccgtctt ggccggagcg cgatgggcga agagcgcgtc gacctgccgg
    11821 ccgaccatga gccggatgat tccatcgatg tcggtgtccg aaactttgcc ggcgccgacc
    11881 cggcggccgt cgcgcagcac ggtgtagcga tcgcagatct ccatcatctc ttcgagccga
    11941 tgggtgacga agatgatgct gagcccctgc gccttgagat cgcgaatgat gcggaacagc
    12001 ttttcgactt ccgccgacga gagggcggag gtcggctcgt ccatgacgat cagccgcgat
    12061 tgcatcgaca gcgcccgggc gatctccacc atctgctgct cggcgacgga gagatcgcgc
    12121 accagcgcgc gcggcttgag gccgagcccg agccgctcgg tgatcgctcg cgtctcggcc
    12181 gccatcttgc gccaattgac gaacggcccc gcgcccggct cgcggccgat gaacacgttc
    12241 tcggcgacgc tcatattcgg cgcgagcgtg aactcctggt agatggtgac gatgccgagc
    12301 cgctgcgcgt cctgcggcga ggccaacata accggcgcgc cggcaagcga aatcgagccg
    12361 gcgtcgggct gctgcgcgcc cgacaagatt ttcagcagag tcgattttcc ggccccgttc
    12421 tcgccgagca gcgcatgaac ttcgccgggc gagacttcga gcccgacatc gtcaagcgcc
    12481 tgcacgccgg gaaagcgctt gctgattccc gacatgcgga ggaggggctc ggtcatggtc
    12541 cggcagtcgg caatcggcag ccggcagtcg gcgagcgcag agggacggtc gggacccccg
    12601 cgggatgcgc ggtcgccccg attgccgact gccgacctgc cgactgccgt atcttcactt
    12661 cacctcgctt aggcgctcgg ccttgtcgag attgtccttg gtgatcgcga ccggcgtcag
    12721 cagggtcact ttctcggccg gctgctttcc gtcgcgcagg aacgccacaa ggatgtccag
    12781 cgccttctgg ctctggccgc ccggcgactg ctcgatcgtc gcggtcatgt tgccggcctt
    12841 gatctggccg agcgcctccg gcagcgcgtc gaagccgatc agcttgacct tgccgataag
    12901 gttgcgcgcc ttcagagcct ccaaggcgcc gagcgccatg tcgtcattgg cgcagacaat
    12961 gacgtcaggc ggcgacgtca tcccgtcgag cgccgtttcg gacatcgaca ggcctttggc
    13021 gcggtcgaag cctgccgtgt cctcgaagac gaacttgtat ttgtccttgg ccttgtcgag
    13081 cacgttgtgc aggcccttgt tgcggtcgat ggccgggctc gatcccgact gaccttgcag
    13141 gttcatcacc ttggcgccgt tcgggaacag cttctcgatg agctcgccct gcgcctcgcc
    13201 gcccttcaca ttgtcggcgc cgacatgggc gagcacgccc ggaaccttgt cgacgcggcg
    13261 gtcgaccgtc acgacgggga ttttggcgtc caccgcttcc tgaaccgccg gcgcgagagc
    13321 gtcgacctcg ttcgggtcga ggaacatgcc gttcacgccc ttggtgatcg ccgcctcgat
    13381 gtcggcggtc tgcttgggcg acgagcgctg cccgtcggat tcgatgatgt cgatgtcccc
    13441 gatcttggcg gcctcggcct tcacttccgc catcatgtga gtgaagaagg ggaacgccat
    13501 gtcgggaaag gtgacgagga tggtcaggtg cttgccggcc gcttgggcag gcgccccgaa
    13561 ggtcagcgcg aggctggcca cgcccgctac gagcgccgct ttggcgacat ttcggaagat
    13621 catcggtttc ctcccgtatg cgctccgccg gagcgcatac ggctcgtcgg cgagcgatta
    13681 agccgcccga accggacggc cgctctttat ccattcgcgg caaagcgcga gtcaagtgaa
    13741 atggctagaa tgttagtggc gcaatttctg gcggagcgga tcgcgcgatg cttcactgtc
    13801 cggtaaggtt aaggcgcgct cggcgttgcg cccatggccg ccccggccga tcttggctct
    13861 tctctttctt cgaagagccc ggcgatggat tacgcaaccc gacttgcgcg gcctttcagc
    13921 ggcgcggcgg ccgaacgtca ggcgcgcgtc tcgcgccgcc ttgcgccttc gcgatttctc
    13981 gccgggccgt tccagcggct cggctatgac agttccgaag cctgggcctg ggagaactac
    14041 cgctcggtcg tgcttggcct cgcccaggcg agccggcggc gcgacggcga gccgacgcgc
    14101 atgctcgaga tcggcggcgg ccgcggccca ttgctgacgc ccgccgaggc gtccgcggca
    14161 gggatcgcgg tgacggtcaa cgatgtcgat gcgcaagaac tggcgatggg tcctcaggag
    14221 ttcgaaaaag cgcagttcga cattgccggc gaaatcgatc cggctttgca cggcaagttc
    14281 gatctcgtgg tctcgcgcat ggtgatggag catgtcggcg atgggcgcaa agcctgggcc
    14341 aatatggccg cgttgctcgc gccgggcggc gttgcgctga gcttccaccc gacgctctac
    14401 gctccgcctt acctcatcaa cctggctttt cccgaagcgc taaccgcgcg cgtcctgcgc
    14461 ttcttcttcc ccaatcgcca tcagggcgac tacccgaaat tcccggcgcg ctacgatttg
    14521 tgcttctccg atccggccaa ggtcgagccg gtcctcaagg cttgcggctt ttcggaagtt
    14581 ctcattgcgc cgttctgggg ccatggctat ttccgccata tcccgctgct gcgcgaattg
    14641 gacgcggcct tgcagcgcgt ggccgaagcg cgcgagtggc gcgggctgac gacctacgcc
    14701 ttcgcgctgg cgcggcgata ggccctattc ccgcgaaagc ttggactcca gtacgctcta
    14761 tccttcatgc gcggcggctc tccggattcc cgctttcgcg tgaatgacag agtccgctac
    14821 gaggctgcgc cgattgtggg ctcgcgtcgc agccagcagt ccgtagagcg cccccagcag
    14881 caggaacaga tggcgccaat gatcggtgtc gatctggaag ccttgcagga tttgcacgaa
    14941 caggcaggac caaatggcga tcgcctcggt ctggtacggc gtgcgctgaa agaccaggcg
    15001 ccagccgagc gcgaccgtcg cggcgatcag cgccgcgtag cataagccgc cgatccagcc
    15061 gtaggaggcg aaggcgttca catagacgtt atgcggatcg acgccctcaa aatggttgcg
    15121 atattgcagc ggaccgaagc cgagaggccg gtcgagcagc atcgggatgg agcgccactg
    15181 gtcgccgaaa cggccggtct ggccgacatc gtagtcctgg ctcagcgaaa aacggatttc
    15241 gaagacgctg cgtatatccg ggatcgacag cgcgatcgcg agcagcacga ccagtatcgc
    15301 ggcgccgagg atagagagaa ttacgatgcg ccggcgcaga gccgatgagg gcgtcgtcaa
    15361 atacgtcaag ccgaccatca tcagcgtcga ggcgatccat acgccccagg cgccgcgcga
    15421 gaagctcaaa aacagcgccg taagcattac gagaagcggc agataggtac gaaagaagcc
    15481 tttggcctgc cttagcagaa tcgcctggaa aagccagatc agcggcggcg ccaggaaagg
    15541 gccgagcacg tttgggtctt tgaacgtgcc ggaggcgcgg ttatagagcg tgaaatgctc
    15601 ggcaaggccg gcgacgttaa agtagccaag aatgccggct aggccggcga tcattcccgc
    15661 gacgacatag ccggaacgga tgacggcgag ccgctccagc ggcgccttgg cgaccaaggc
    15721 ggcgaagaag accgccgtca ccgatatata gagcgagatc gccgagaaca tgtagctctc
    15781 gcgctgatcg atgtaaggca agagcgacag cgcgccgccg acattgaaca aagccagccc
    15841 gacgatcagc ggcgccatgg cgcgatcgaa caggaggtcg cgccagccaa aagccagcag
    15901 cgccggaatg aacatcaact cgtacggcga aggctcgatg ctggcgagcg cgccgctggc
    15961 gacgaacagc cagagcagcg cgcgctgcag cttgccgatc gagaggctcg cggtgcgggg
    16021 acgcgtcctg acggtttccg cggcggtcat tgtcggtaac ccggagaccg ggcgcccccg
    16081 ccgcgcctag ccgatgtcat tcccgcctgc gcggaaacga caagcaaggc tgacccgcaa
    16141 gtcggcgacg ggcgcggtcg cggcgacggg gagtccatac tagtacgcgt tcttcgtctt
    16201 gatcagcgaa tagggcgtga tcgcgagaat gtagagatcg agcaggatcg accagttctc
    16261 gatatagtgg aggtcgtgct cgacgcggtg ctggattttt tccggcgtat cggtctcgcc
    16321 gcgccagccg ttgatctgcg cccagccggt aatacccggc ttgacgcgat ggcgggcgaa
    16381 atagccatcg acgacctcgt catattggcg ttcggcggcc ttggcatgca gcgcatgcgg
    16441 ccgtgggccg acgagcgaaa gattgccttt gaaaacgaca ttgaagagct gcggcaattc
    16501 gtcgagcgag gtcttgcgga tgaagcggcc gacgcgcgtg acgcgcggat cctccttggt
    16561 cacgagcttt gacgcatcga aatcgagcat gtcggtgcgc attgagcgga atttgaagac
    16621 ttcgacaacc tcgttgt
//
LOCUS       DATGJW010000492         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1232158, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000492 DATGJW010000000
VERSION     DATGJW010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1232158"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(72..1883)
                     /locus_tag="VKU03_14780"
     CDS             complement(72..1883)
                     /locus_tag="VKU03_14780"
                     /inference="COORDINATES: protein motif:HMM:NF019474.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase fold domain-containing
                     protein"
                     /protein_id="HLJ72582.1"
                     /translation="MALLIERKALGRADGIIAADLSDKMNAIQAEQTYAEDDGSYDLN
                     HARLWIDEYSSLASRGFEPLGVSAEDRIVTTPLGPVTTRVYNPDEEGPILVFVHGGGW
                     AIGGVKSHDHIARWLAAETGFRVVQIEYALAPEHPYPAAMNQIAAVLSAMLAESRGAP
                     VLAAGDSAGANLAATAILSLTKDQRRQLAGFVSIYGAYAPEMNLSSHRLYGDGRFGLS
                     EAQMRWFWNLYAPHLPPEERAKRLSPLTADLSDFPPTLCIGAECDLLLDDTLAFYGEL
                     TKAGADVSLSLWSSLPHGCMHFVGTVPSVTEAASAIVQFVIARGRDPKARARLAPEGA
                     SIPAQKLGADAERAAPNPEALVDVEPLFTTSRSRLHGSLAHRIAGGIIKGELPSGSLL
                     PREESAGEAFGVSRSAYREAIRTLAAKGMVSALPKVGTKVAPRSAWHILDPDILAWHF
                     EMAPNEAFIRNLFELRKIVEPSAAALAAMRRNEQELSRLADALSRMARANPRTGAWLN
                     AIVAFHQELLSAGRNEALTSLWPAVQTTLRWSVKLQMMLPSLSLAHDPVADHARVFEK
                     VASQNAEGTLTEMALLIEAALADTLANMKRIETIKAA"
     gene            2083..>2337
                     /locus_tag="VKU03_14785"
     CDS             2083..>2337
                     /locus_tag="VKU03_14785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72583.1"
                     /translation="MIGPARTLLLALSAIALAAAASPAQAEDKPIAIAFVSGPLNDSF
                     FPPLYQGAQDAAKALGVKLNYIPIDEADIEATSARTMQTAI"
BASE COUNT          411 a          762 c          769 g          395 t
ORIGIN      
        1 gcgggcatgg cggcgctcct cttttgacaa agagaatgaa cgcgccaagc cgccgccggg
       61 tccgcccgcg ctcaggccgc cttgatcgtc tcgatgcgtt tcatattggc cagcgtgtcc
      121 gccagcgcgg cttcgatgag cagcgccatc tcggtcagcg tcccctcggc gttttgcgag
      181 gcgactttct cgaagacgcg ggcgtgatcg gcgacggggt catgcgccaa gctcaagctc
      241 ggcagcatca tctgcagctt gaccgaccag cggagcgtcg tctggacagc cggccagagc
      301 gaggtcagcg cctcgttgcg gccggcgctg agcaattcct ggtggaacgc cacgatcgcg
      361 ttgagccagg cgccggtgcg cggattggcg cgcgccatgc gcgaaagcgc gtcggcgagc
      421 cgtgaaagct cctgctcgtt gcgccgcatc gcagcgagcg ccgcggcgct cggctcgacg
      481 atcttgcgca gctcgaagag attgcgaatg aaagcctcgt tcggcgccat ttcgaaatgc
      541 caggcgagga tgtcgggatc gagaatgtgc caagctgagc ggggcgcgac cttcgtgccg
      601 actttcggca gcgccgagac catgcccttg gcggccaagg tgcggatcgc ctcgcgatag
      661 gcgctgcgcg acacgccgaa cgcttcgccg gcgctctcct cgcgcggcag cagcgacccc
      721 gagggcaatt cgcccttgat gatgccgccg gcgatgcgat gagcaagcga gccatgcagc
      781 ctggatcggc tggtcgtgaa cagcggctcg acatcgacaa gcgcctcagg attcggcgcg
      841 gcgcgttcgg cgtcggcgcc aagcttttgc gccgggatgc ttgcgccttc cggcgcgagg
      901 cgggcgcgcg ccttcggatc gcgcccgcgc gcgatgacga attgcacgat cgcgctcgcg
      961 gcctcggtga cgctcggcac ggtcccgacg aaatgcatgc agccatgcgg caggctcgac
     1021 caaagcgaca gcgacacgtc ggcgccggct ttggtgagct cgccgtaaaa ggccagcgta
     1081 tcgtcgagca agagatcgca ctcggcgccg atgcagagcg tcggcggaaa atccgacaag
     1141 tcggcggtca gcgggctgag ccgcttggcg cgttcctcgg ggggaagatg cggcgcataa
     1201 agattccaga accagcgcat ttgcgcttcc gaaaggccga accgcccgtc gccataaagc
     1261 cgatgggacg agagattcat ctccggcgcg taagcgccgt agatcgagac gaagccggca
     1321 agctggcggc gctgatcttt cgttaggctg aggatcgcgg tcgcggcgag attggcgccg
     1381 gccgaatcgc cggccgccag gacgggcgct ccgcgagact cggcgagcat ggccgagagc
     1441 accgccgcga tctggttcat cgcggcgggg tacggatgct ccggcgccag cgcatattct
     1501 atctggacga cgcgaaagcc ggtttctgcg gcgagccagc gcgcgatgtg gtcatggctt
     1561 ttgacgccgc cgatcgccca gccgccgcca tggacgaaga ccaggatcgg cccctcttcg
     1621 tcggggttgt agacgcgcgt cgtcaccggc ccgagcggcg ttgtgacgat ccggtcctcg
     1681 gccgagacgc cgagcggctc gaagccgcgc gaggcgaggc tcgaatattc gtcgatccag
     1741 agccgggcat ggttcagatc gtaggagccg tcatcctccg cataagtctg ctccgcctgg
     1801 attgcgttca ttttgtcgga caaatctgcc gcaatgatgc cgtctgcgcg gccgagcgcc
     1861 tttctttcga tcaggagagc catctgaact ccaggattag gcgcgtcatc tcccaccttt
     1921 catgacacaa agccgacatt aacctcaatt gaggttgacg atgtccgaca aattcattac
     1981 ccaaagagaa gccggtcggc aactgccgcg ccgcgagcgc cataaaacag acccgacgcg
     2041 tccgggctcg ggatctgggc attcgatttg gaggaacgcc atatgattgg acccgcccgc
     2101 actcttcttc tggctttgag cgcgatcgcc ctcgccgcgg cggcttcgcc ggctcaggcc
     2161 gaagacaagc cgattgcgat cgccttcgtc tccggtccgc tcaacgattc cttcttcccg
     2221 ccgctctatc agggcgcgca ggacgccgcc aaggcgctcg gcgtcaagct caactacatc
     2281 ccgatcgacg aggccgatat cgaggcgacc tcggcccgca ccatgcagac cgcgatc
//
LOCUS       DATGJW010000493        11162 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_823299, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000493 DATGJW010000000
VERSION     DATGJW010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11162)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11162)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11162
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823299"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            333..530
                     /locus_tag="VKU03_14790"
     CDS             333..530
                     /locus_tag="VKU03_14790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72584.1"
                     /translation="MDSPQDELAGLKRLLAALKSRNMTIYEGRLDVTPRETWKLENNI
                     SFLESILKREKSRPLSANKER"
     gene            complement(643..2361)
                     /locus_tag="VKU03_14795"
     CDS             complement(643..2361)
                     /locus_tag="VKU03_14795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004110513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase family protein"
                     /protein_id="HLJ72585.1"
                     /translation="MTFIQDKPEDFAAAALDGFCSAYPRWVRRVQGGAVRATQTPLGK
                     VAIVIGGGSGHYPAFAGYVGPGLADGAVAGDIFASPSARSAVNVIRAVERGAGVLLGF
                     GNYAGDVLHFGAAAERLAADGVDMRILRVTDDVASAPRDQRTLRRGVAGDVVVFKIAG
                     AAAERGLTLDDVERCARRANERTVSFGVAFSGCTLPGANAPLFTVPAGRMAVGLGIHG
                     EPGVAEAPIPSAAELAKMLVDRLLEERPERAGSRVATLLNGLGCVKYEELFVLWTHVE
                     ERLQAAGLTPVAPEVGEFVTSLDMAGCSLTLTFLDDELEDLWLAPADAPAFRRGAIIA
                     AEPAPPIAAESEREPPVPPASEASRADGRCVASLFGRIADAMRQAEEQLGRIDAVAGD
                     GDHGQAMSRGSAAAKEAAERACAEGAGAATVLARAGDAWADRAGGASGALWGALLRAW
                     SGAFADDEPIALERLAAGAMAARETVARLGGAKIGDKTLLDALDPFATSLAQDARSGR
                     SLASVWRKAAGAARWAAEGTAQLTPKLGRARLHGSRSLGHPDAGALSLALCAETVATF
                     LENSPT"
     gene            complement(2404..3240)
                     /locus_tag="VKU03_14800"
     CDS             complement(2404..3240)
                     /locus_tag="VKU03_14800"
                     /inference="COORDINATES: protein motif:HMM:TIGR01459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01459 family HAD-type hydrolase"
                     /protein_id="HLJ72586.1"
                     /translation="MTTSSRFLSIAAKFDGVFMDQYGVLHDGSRVYPGAEEALRGLKS
                     LGVKVAILSNSGRSGEENAQRMAKLGVGPDLYDRFVTSGDAAEALLKGEESPAPLTES
                     TRCLALSSDGGPDFAQALGVRSVRDGGEADLVVISGSQGDRFSLDHYRRILAPAAARK
                     VPCLCANPDRFMLTGAGVAFGAGRIAELYEELGGSVIWVGKPYLPIYRTAAALIGVAD
                     PARLLCVGDSVEHDVVGAHRFGAAALLVRTGILAQFDERALAAEFEKHQATPEYILAD
                     LR"
     gene            complement(3237..4496)
                     /locus_tag="VKU03_14805"
     CDS             complement(3237..4496)
                     /locus_tag="VKU03_14805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009208821.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLJ72587.1"
                     /translation="MRPCAIGVDVGTSGVRAAALALNGAVCGQSSVAMASLGDSRAPM
                     TWRRAVSTCLAELGATCDLSGVRGLAVDGTSGTMVAVDRQGAPIGVPLMYDAPCEVRD
                     VLRAIEAQAPAESAARGRTSALARVLILQKRPGAHRVLHQADWIAGWLLGRFDRSDAN
                     NALKTGYDPIAGRWPDWIERAGADLDKLPIVVEPGEPLGPIGAEGAALGLPAAAIVHA
                     GTTDGCASFLATGAAEIGDAVTALGSTLVIKLLCDKPISAPQYGVYSHRIGDAWLAGG
                     ASNTGGAVIERLFGRDRLPALSAALRPDRPTGLHYYPLSAPGERFPINDPDLPPRLEP
                     RPADQKTFFQGVLEGVAEIEALAYRKLAELGAPVVRSIRTVGGGAANAGWTEIRRRIL
                     NAPFAPARSQEACVGVAQLVLRSMRPA"
     gene            complement(4493..5557)
                     /locus_tag="VKU03_14810"
     CDS             complement(4493..5557)
                     /locus_tag="VKU03_14810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006201968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HLJ72588.1"
                     /translation="MTSAVGKDAEFRALLQLSAALGANPLRTQAAGGNTSIKRNGVMW
                     IKASGAWLADALQRDIMTPIRLAPLLEAMEAGDARAENAVAFIDGARGALGLRPSIET
                     SVHAALPQAVVVHIHCVDTIALAVRADAEAAIAARLPDLRKGEWIFIPYVKPGLPLAR
                     AIRSRLTEKTQVIVLGNHGLVVAAETVAEAAVRTERICRAFAAIPKAAPAPNLDRLAS
                     FAEGGDYRLPHDSAAHATATDPRRLAWARLGSLYPDHVVFLGPGIVEGTIVGGRLRAP
                     ALDLPAPMLVLPGLGVALDRGAPKGTDEMARCLAEVTGRIVDGAPVVPLAPAQETELV
                     NWEAEKYRRSLNAAPSASRP"
     gene            complement(5554..7068)
                     /locus_tag="VKU03_14815"
     CDS             complement(5554..7068)
                     /locus_tag="VKU03_14815"
                     /EC_number="1.1.5.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962096.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009331 - glycerol-3-phosphate
                     dehydrogenase complex [Evidence IEA];
                     GO_function: GO:0004368 - glycerol-3-phosphate
                     dehydrogenase (quinone) activity [Evidence IEA];
                     GO_process: GO:0006072 - glycerol-3-phosphate metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycerol-3-phosphate dehydrogenase"
                     /protein_id="HLJ72589.1"
                     /translation="MRDLGQFDLIVVGGGVNGAGIARDAVGRGLSVLLCEKDDLAEGT
                     SSRSGKLVHGGLRYLEYYEFRLVREALIEREVLLKAAPHIVWPMRFVLPHSPEQRPAW
                     MIRFGLFLYDHLGGRKTLPPTRNLDLTNAPEGRPLKRSFKRAFEYSDCWVDDSRLVVL
                     NAVDIRQRGGTVLTRTPMVSGRRSRDHWTIELQDARRNESLTARGKILVNAAGPWVED
                     VLQRAGRNGESRVRLVKGSHIVTRKFWDGEQAYLLQHDDKRVIFVNPYEGDLALIGTT
                     DIPFDDPAEDAEITAEEIDYLLAIVARYFEKAPTAKDVRHAFSGVRPLYDDKAANPSA
                     VTRDYVFDIDAPGDFAPILSIFGGKITTYRKLAEHALEKLAPFLPQMGANWTDKAPLP
                     GGDIPDADFPRFLDDLRRRRPWLPDDVSLGYARRYGLRAEQLLDGAQSLDDLGRHFGA
                     TFYEREARFLVETEWAMMADDILDRRTKHGLHLTALQRDDFGAWLKSVSMALTS"
     gene            complement(7097..8152)
                     /locus_tag="VKU03_14820"
     CDS             complement(7097..8152)
                     /locus_tag="VKU03_14820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008962094.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-hydroxyacid dehydrogenase"
                     /protein_id="HLJ72590.1"
                     /translation="MAAKRRTATISIIGDRFMLPAMFEKAIREKCDGELDIRSHEMPW
                     PDEPMQHGYAEPGMDGLKEYQGTADEIVAYADGADIVVTHLAPFSAGILDRLPALKLI
                     AVSRGGPVNIDMKAARERRVLVVNTPGRNASAVAEFTIGAILAETRLITRGHDSLRRG
                     EWRGDLYRADLTGEELSEMTIGVIGYGHVGTKVVRLLKVFGCRVLVCDPYVQLSADDL
                     RDGVEQVSIDRLLRDSDVVTLHARVTAETRGFIGAAQFAAMKRGAYFVNTARGPLVDY
                     AALTEALKSGHLRGAALETFAVEPTPPDLELLRLENVTLTPHIAGASVKTVKYAAAAA
                     AEEVRRYLAGEGPLNPC"
     gene            complement(8155..9720)
                     /locus_tag="VKU03_14825"
     CDS             complement(8155..9720)
                     /locus_tag="VKU03_14825"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019200 - carbohydrate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FGGY-family carbohydrate kinase"
                     /protein_id="HLJ72591.1"
                     /translation="MTRDILIGVDAGTSVIKSVAFSLAGEQIAAAARPNVYETPGPGQ
                     VEQDMARTWTDCVATIRELVERTPNLCERVAGLAVTAQGDGAWMIDSDGEPVGGGLLW
                     LDSRAAALIDDYLKTEAYQRHYRFTGSGVNACMASGQLAWMKKWQPERIAKAATCFHC
                     KDWLYFKLTGARVTDPAEGNFTFGNFRTRQYELSILDGMGLKDCARLLPPMIEGSRES
                     GRLSAAAAAATGLPEGLPISLAYLDVICTALGGGLYDCSGKVGVSIIGSTGMHMRYAP
                     TLDDVRLNDERSGYTMCFPVESSCASMQSNMAATLNIDWLLDIARQAAELGGVETSRA
                     TLLSGLDEKILAAAPGQAIYHPYIFEAGERGPFLNPNARAQFSGLSTGTNLLGLIRAV
                     YEGLAFAARDCYLAAGAIPQEVRLTGGAARSKAMRAIVASVLNANVRSLAREEIGAAG
                     AAMMAATATGVYKDINACVEAWVTPSLGDVTAPDPGVSRFYSNLYPVYRSIRERMPPA
                     WAALGQVRQEYAT"
     gene            complement(9761..10885)
                     /locus_tag="VKU03_14830"
     CDS             complement(9761..10885)
                     /locus_tag="VKU03_14830"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010969794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72592.1"
                     /translation="MPDIALSLNNVDKVYQRRGRPPVHAVKALSMTVERGEIVALLGS
                     SGCGKTSTLRMIAGFEAVSNGDIAIAARRIDQLPPARRGVAMAFEGYSLYPPLTVRDN
                     IAFALKSARLNAKEVEKSVAEISRLVEIEEILDRYPSSISGGQQQRASLARALVRRAD
                     LYLLDEPMGQLEPQLRAVLRARLKSLLVERRMTSIFVTHDQTEANALADRIAVMEGGL
                     LQQFASQKELREKPANLFVATFIGEPPMNVFEAAVENRESMRFAVKDGPTFDFASAEL
                     PPNVRQAIGGRDRAVIGIRPHAIRLGAGANEARIVSNQWLGDQSHLAMDVAGKLLVAV
                     SHKPIAAKAGEAIRYGVGAADLHVFDAATAAAVSHGLETA"
     gene            complement(10891..>11162)
                     /locus_tag="VKU03_14835"
     CDS             complement(10891..>11162)
                     /locus_tag="VKU03_14835"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HLJ72593.1"
                     /translation="CMLGLRPESILLGSHPSPESFDVDLVAATPLNEKTLLLLRTADG
                     RELLASEPSADEAPRRHGPAYARFDPSAILLFDAASGRRIAPQSA"
BASE COUNT         1961 a         3709 c         3704 g         1788 t
ORIGIN      
        1 gaacgcttgg cctgccgcca agggcaagcg acggacgcaa ccggacgtac tctccagggc
       61 tgccctcttc tccagggttg gcggggccgc gcattgtcct taccgcaaat cttcgcctcg
      121 aattgtgata gactgaaatg agtgagtcgt gcggcttgtc gatcttagga cgtcaccatg
      181 cccgccttgc tctggagcca gcttggcgcc tcggacggat tggttcgcct tgctcaaagc
      241 gggccgaacg ctgaagggct agcctcgacc gtccgcttgc cgccgcgttg gcgggcctta
      301 gtcctgccga tcccctagcg aggaagcggc ggatggactc tccccaagac gaattggctg
      361 gactgaaacg cctgcttgcc gcgctgaaat ccagaaacat gacgatctat gagggaaggc
      421 tcgacgtcac gccccgagaa acttggaaat tagaaaacaa catcagcttt ttagagtcga
      481 ttctcaagcg cgagaaatcg cgtcccctct ccgcgaacaa ggagcgctga ttcgatcagc
      541 gcgttcaacg cgcgacggaa agcgagaagg cgtgattgtg ccgtagcgct gcggcgtgtt
      601 tgcgtccgtc gcgcgcgtcg aagatccgga acgcgcaaag ctttaggtcg ggctgttttc
      661 cagaaaagtc gcgacggtct cggcgcaaag cgccagcgac agcgctccgg cgtcgggatg
      721 accgaggctc cgcgagccgt gcagccgcgc gcggcctaac ttgggcgtca attgcgcggt
      781 cccctccgcc gcccatcgcg ccgccccagc ggccttgcgc cagaccgagg cgagcgatcg
      841 tcccgaccgc gcatcttgcg cgagcgaagt cgcgaaaggg tccagggcgt cgagaagggt
      901 cttgtcgccg atctttgcgc cgccgagccg cgcgactgtc tcccgcgccg ccatggcccc
      961 ggcggcaagc cgttcgaggg cgatcggctc gtcatcggcg aaggcgccgc tccaggcgcg
     1021 caaaagcgcc ccccagagcg cgcccgaggc cccgccggcg cggtcggccc aggcgtcgcc
     1081 cgcgcgcgcc aggacggtgg cggcgcccgc tccctccgcg caggctcgct ccgccgcctc
     1141 cttcgccgcc gccgaaccgc ggctcatcgc ctgcccatga tcgccatcgc ctgccacggc
     1201 gtcgatccgc cccaattgct cctcggcctg gcgcatcgca tccgcgatgc gaccgaacaa
     1261 agaggcgacg catcggccgt ccgcgcgcga cgcttcgctc gccgggggaa ccggcggttc
     1321 gcgctcgctc tccgccgcga tcggcggcgc gggttcggcg gcgatgatcg cgccgcgtcg
     1381 aaacgccggc gcgtccgccg gcgccagcca tagatcttca agctcgtcgt caagaaacgt
     1441 gagcgtcagg gagcagccgg ccatatccag actggtgacg aactccccga cctccggcgc
     1501 gaccggcgtc agacccgcgg cttgcaaacg ctcctcgaca tgcgtccaca acacgaagag
     1561 ctcttcgtat ttgacgcagc ccagcccgtt gagcagcgtg gcgacgcggc ttccggcgcg
     1621 ctccggcctt tcttccagca gccggtcgac cagcattttg gcgagttcgg cggcggacgg
     1681 aatcggcgct tcggcgacgc ccggttcgcc gtggatgccg aggccgaccg ccatgcggcc
     1741 ggcgggaacc gtgaagagcg gcgcgtttgc gcccggcaac gtgcagccgc tgaaggcgac
     1801 gccgaacgat acggtgcgct cgttcgcccg gcgcgcgcaa cgctcgacat cgtcgagcgt
     1861 caagcctcgc tccgctgcgg cgccggcgat cttgaagacg acgacatcgc cggcgacgcc
     1921 gcggcgcaat gtccgttggt cgcgcggcgc gctggcgaca tcatcggtca cgcgcaagat
     1981 gcgcatatcg acgccgtccg ccgccagccg ctcggcggcc gcgccgaaat gcaggacatc
     2041 gccggcgtaa ttgccaaagc cgagcagaac gccggcgccg cgctcgacgg cgcggatcac
     2101 attgacggcg ctgcgcgccg aaggcgacgc gaaaatgtcg ccggcgactg cgccgtccgc
     2161 caagcctggg ccgacatagc cggcaaaagc cggatagtgg cccgagccgc cgccgataac
     2221 gatcgcgact ttcccgagcg gcgtttgcgt cgcgcgcacg gcgccgcctt ggacgcggcg
     2281 gacccagcgc ggataagccg aacaaaaacc gtcgagcgcg gccgcagcga aatcttccgg
     2341 cttgtcttgg atgaaggtca cgatcctctc tccttcgcca ggcgaaattc gtctcgagcc
     2401 gcttcagcgc aagtcggcga ggatgtattc cggcgtggcc tgatgctttt cgaactcggc
     2461 ggcaagcgcg cgctcgtcga attgcgccag gattccggtc cgcaccaaga gcgcggcggc
     2521 gccaaagcgg tgggcgccga cgacgtcatg ctccacgctg tcgccgacgc agagaaggcg
     2581 cgccggatcg gcgacgccga tcaaagccgc cgccgtccga tagatcggca ggtacggttt
     2641 gccgacccag atcaccgagc cgccgagctc ctcgtagagc tcggcgatgc ggccggcgcc
     2701 gaaagcgacg cccgccccgg tcagcatgaa ccgatccgga ttggcgcaaa ggcacggaac
     2761 cttgcgcgcc gcggccggcg ccaggatgcg ccggtaatga tcgagcgaga agcggtcccc
     2821 ctggctgccg ctgatcacga cgagatcggc ttctccgccg tcccggacgg agcgtacgcc
     2881 gagcgcctgc gcgaaatcgg ggccgccgtc cgacgaaagc gccaggcatc gcgtcgattc
     2941 ggtgagcggc gcgggcgatt cttcgccttt gaggagcgcc tcggcggcgt ctcccgatgt
     3001 gacgaaacga tcgtaaagat cggggccgac gcccagcttc gccatgcgtt gcgcgttctc
     3061 ttcgccggaa cggccggaat tcgaaaggat tgcgaccttg acgccaagcg acttcaatcc
     3121 gcgaagcgcc tcctcggcgc cgggataaac gcggcttccg tcgtgcaaga cgccgtattg
     3181 atccatgaac acgccgtcga actttgccgc gatggagagg aagcgcgagg aggtcgtcac
     3241 gccggcctca tcgagcgaag aaccaattgc gcgacgccga cgcaagcctc ctgggagcgc
     3301 gccggcgcga aaggcgcgtt caggatgcgg cgccggatct cggtccagcc ggcattcgcg
     3361 gcgccgccgc caacggtacg gatcgagcga actacgggcg cgccgagctc ggcgagcttg
     3421 cgataggcga gcgcctcgat ttccgcaacg ccttcgagga cgccctggaa gaaggtcttt
     3481 tggtcagccg gccgcggctc gagccgcggc ggaagatcag ggtcgttgat cggaaagcgc
     3541 tcgcccggcg cgctcagcgg atagtagtga agcccggtcg ggcgatccgg cctcagcgcg
     3601 gccgaaagag ccggaaggcg atcgcggccg aacaggcgct cgatcacggc gccgcccgta
     3661 ttcgacgcgc ccccggcgag ccacgcgtcg ccgatgcgat ggctgtagac gccatattgc
     3721 ggggctgaga tcggcttgtc gcaaagcagc ttgatgacca gggtcgaccc cagcgccgtc
     3781 accgcatcgc cgatttcggc tgcgccggtg gcgagaaacg aggcgcagcc gtcggtggtt
     3841 ccggcatgga caatggcggc agccggaagg cccaacgccg cgccttccgc cccgatcggg
     3901 ccgagcggct cgccaggctc gacgacaatt ggcagcttgt cgagatcggc gccggcgcgt
     3961 tcgatccaat ccggccagcg ccccgcgatc ggatcgtacc cggttttcag ggcgttgttg
     4021 gcgtcgctgc gatcgaaccg tccgagcagc cagcccgcga tccaatcggc ctggtggagg
     4081 acgcgatggg cgccgggcct cttttgcaag atcaaaaccc gcgccagcgc ggatgtgcgg
     4141 ccgcgcgcgg cgctctccgc gggcgcttgc gcctcgatcg cgcgcaacac gtcgcgaacc
     4201 tcgcaaggcg cgtcgtacat cagcggcacg ccgatcggcg cgccttggcg atcgaccgcg
     4261 accatcgtgc ctgaagtccc gtccaccgca aggccgcgca cgccgctgag gtcgcatgtc
     4321 gcgccgagct cggccaggca ggtggatacg gcgcgccgcc acgtcattgg cgccctggag
     4381 tcgccgagcg acgccatggc gaccgaactc tggccgcaaa ccgcgccgtt caaggccaaa
     4441 gctgcagcgc ggacgccgga cgtgccgaca tcgacgccga tcgcgcaagg cctcatggcc
     4501 tgctggccga cggcgccgcg ttcaggctgc ggcgatattt ttcggcttcc caattgacga
     4561 gctccgtctc ctgagccggg gcgagcggaa cgacgggcgc gccgtcgaca atgcgccctg
     4621 tgacctcggc gaggcagcgc gccatctcgt cggttccctt cggcgcgccg cgatcgagcg
     4681 cgacgcccag cccaggcaag accagcatgg gcgccggcag atcaagcgcc ggggcccgca
     4741 gacgcccgcc gacaatcgtc ccttcgacaa tgccggggcc caagaagacg acgtgatccg
     4801 gatagagcga gccgagccgc gcccacgcga gccggcgcgg atcggttgcg gtcgcgtgcg
     4861 cggctgaatc atggggaagg cggtagtcgc cgccttcggc aaaagacgcg agccggtcca
     4921 ggttgggcgc cggcgcggcc ttgggaatgg ccgcgaaggc tcggcatatc cgctcggtgc
     4981 gaaccgcagc ttcggcgacc gtctcggcgg cgacgacgag gccatgattg ccgagaacga
     5041 tcacctgggt tttctcggtc agccgggagc ggatcgctcg ggcgagaggg aggcctggct
     5101 tgacataggg aatgaagatc cactcgccct ttcggagatc gggcaggcgc gctgcgatcg
     5161 ccgcctcggc gtcggcgcgc accgccagcg cgatcgtatc gacgcaatga atatgaacga
     5221 cgacggcctg cggcagcgcg gcgtgcacgc ttgtctcgat cgagggacgg aggcccaaag
     5281 cgccgcgcgc gccatcgatg aaagcgacgg cgttttcagc gcgcgcgtcg ccggcctcca
     5341 tggcttcgag cagcggggcg aggcgaatcg gcgtcatgat gtcgcgctgc aaagcgtcgg
     5401 cgagccaagc gcccgacgct ttgatccaca tcacgccgtt gcgcttgatc gacgtgttgc
     5461 cgccggccgc ctgcgtacgc agcggattgg cgccaagcgc ggccgagagc tgcaagaggg
     5521 cgcgaaactc cgcatcctta ccgacggcgg aggtcatgac gtcagcgcca ttgaaacgga
     5581 tttgagccaa gccccgaaat cgtcgcgctg gagcgccgtc aaatgaaggc catgcttcgt
     5641 ccgccgatcg agtatgtcgt cggccatcat cgcccattcc gtctcgacca ggaaacgcgc
     5701 ctcgcgttca tagaaggttg cgccgaaatg acggccaagg tcgtccagcg attgcgcccc
     5761 gtcgagaagc tgctcggccc gaagcccata gcgtcgagcg tagcccaaag acacgtcatc
     5821 cggcagccat ggacgccggc ggcgcagatc atcgaggaag cgcggaaaat cggcgtcggg
     5881 aatatcgccg ccggggagcg gcgccttatc ggtccagttc gcccccattt gcggcaggaa
     5941 cggcgccagc ttctccaagg cgtgctcggc gagtttccgg taggtggtga tcttgccgcc
     6001 gaaaatcgac aggatcggcg cgaaatcgcc cggcgcgtcg atgtcgaaga cataatctcg
     6061 ggtgacggcg ctgggattgg cggctttgtc gtcatagagc gggcgaacgc cggaaaaggc
     6121 gtggcgcacg tccttggccg tcggcgcttt ctcgaaatag cgcgccacga tcgccaaaag
     6181 gtagtcgatc tcctcggccg tgatctccgc atcctcggcc ggatcgtcga agggaatgtc
     6241 cgtcgtcccg atcagagcga gatcgccctc ataggggttg acgaagatga cgcgtttatc
     6301 gtcatgctgc aagaggtacg cctgctcgcc atcccagaat ttgcgcgtga cgatatggct
     6361 gcctttgacg agacggacac ggctctcgcc attgcggccg gcccgctgca gcacgtcttc
     6421 gacccatggt ccggcggcgt tgaccaggat tttgccgcgc gccgtcaaag actcgttgcg
     6481 gcgcgcgtcc tgcagctcga tcgtccagtg atcgcggctc cgccgtcctg agaccatcgg
     6541 cgttcgggtg agcaccgtgc cgccgcgctg gcggatatcg acagcattga gcacgaccag
     6601 cctggaatcg tcgacccagc aatcggaata ttcgaacgcc cgcttgaaag aacgtttcag
     6661 cggccggcct tccggcgcgt tcgtcagatc gagatttcgc gtcggcggca acgtcttgcg
     6721 gccgccaaga tgatcgtaga gaaaaaggcc gaagcggatc atccaggccg ggcgctgctc
     6781 gggactatgc ggcaggacga agcgcatcgg ccagacgata tgaggcgcgg ccttgagcag
     6841 cacttcgcgc tcgatcagcg cttcccgcac caggcggaat tcgtaatatt cgagataccg
     6901 caagccgcca tggacaagct tgccggaacg ggacgatgtg ccttcggcca ggtcgtcctt
     6961 ttcgcaaagc aggaccgaga ggccgcggcc gaccgcgtcg cgggcaatac cggcgccgtt
     7021 cacgccgccg ccgacgacga tcagatcgaa ttgtccgagg tccctcatct tgagctttct
     7081 tcgcctaacg ccgtcctcaa cagggattga gcggaccttc gccggcgagg tagcgtcgca
     7141 cctcctcggc cgcggcggcg gcggcgtatt tgaccgtctt gaccgaagcg cccgcaatat
     7201 gcggcgtcaa ggtcacgttc tcgagccgca gcaattcaag atcgggcggc gtcggctcga
     7261 cggcgaacgt ctcgagcgcc gcgccgcgca gatggccgct tttcaaagcc tcggtcaagg
     7321 cggcgtaatc gacgagcggt ccgcgcgcgg tattgacgaa ataggcgccg cgcttcatcg
     7381 ccgcgaattg cgccgcgccg atgaagccgc gcgtctcggc ggtcacgcgg gcgtgcaagg
     7441 tcacgacgtc gctgtcgcgc agcagccgat cgatcgagac ctgctcgact ccgtcccgca
     7501 gatcgtcagc ggagagctgg acataggggt cgcacaccag gacccggcag ccaaagactt
     7561 tcagcagccg aacgactttc gttccgacat gcccatagcc gatcacgcca atggtcattt
     7621 ccgaaagctc ttcgccggtc agatcggcgc ggtagagatc gccgcgccat tcgccgcgcc
     7681 gcaagctgtc gtgcccgcgg gtgatgagcc gcgtttcggc gagaatggcg ccgatggtga
     7741 attcggcgac cgccgaagca ttgcggccgg gcgtgttgac gaccagcacg cggcgctcgc
     7801 gcgccgcttt catgtcgata ttgaccgggc cgccgcgcga cacggcgatc aatttgagcg
     7861 ccggcagccg gtcgaggatt ccggcggaaa agggcgccag atgggtgacg acgatatccg
     7921 ccccgtccgc ataggcgacg atctcgtccg ccgttccttg atattctttg agtccatcca
     7981 ttcccggctc agcataaccg tgctgcatcg gctcatccgg ccaaggcatt tcgtggctgc
     8041 ggatgtcgag ctcgccgtcg catttctcgc gaatcgcctt ctcgaacatc gccggcaaca
     8101 tgaagcggtc gccgatgatc gagatggtcg cagtccggcg cttcgccgcc atgctcacgt
     8161 cgcatactcc tgtctgactt ggccgagcgc cgcccaagcc ggcggcattc tctcgcggat
     8221 cgagcggtag accggataga ggttcgaata gaaccggctg acacccggat cgggcgccgt
     8281 gacatcgccg agtgacggcg tcacccaagc ctctacacaa gcattaatat ccttgtagac
     8341 gccggtcgcc gtcgccgcca tcatcgccgc gcccgcagcg ccgatttcct ctcgggccag
     8401 agacctgaca ttggcgttca gcaccgaagc cacgatcgcc cgcatcgcct tggagcgcgc
     8461 cgcgccgccg gtcagccgca cttcttgcgg aatggcgccg gcggcgagat agcagtcgcg
     8521 cgccgcaaag gcgagaccct catagaccgc gcggatcagg ccgagcaggt tcgtcccggt
     8581 ggagaggccg ctgaattgcg ctcgcgcgtt cggattgaga aacggcccgc gctcgccagc
     8641 ctcaaaaatg taaggatgat agatcgcctg gcccggcgcg gcggcgagga ttttctcgtc
     8701 cagtccggag agcaaagtcg cccggctggt ctcgacgccg ccgagctccg ccgcttggcg
     8761 ggcaatgtcg aggagccaat cgatgttgag cgtcgccgcc atgttcgact gcatcgacgc
     8821 gcaagagctt tcgaccggaa agcacatcgt gtagccggaa cgctcgtcat tgagccgaac
     8881 gtcgtcgagc gtcggcgcat agcgcatatg catgccggtc gagccgatga tcgagacgcc
     8941 aaccttgccg ctgcaatcgt acagaccgcc gccaagcgcg gtgcagataa cgtcgagata
     9001 ggcgagcgag atcggcaggc cttcgggcaa gccagtcgcc gcggccgcgg cggccgacaa
     9061 gcgaccgctt tcgcgtgacc cttcgatcat cggcggcaac aggcgcgcgc aatccttaag
     9121 tcccatgcca tcgaggatcg aaagctcgta ttgccgcgtg cgaaagttgc cgaatgtgaa
     9181 attgccctcg gccgggtccg tgacccgggc gccagtcagc ttgaagtaga gccaatcctt
     9241 gcagtgaaag caggttgcgg ctttggcgat ccgctcgggc tgccatttct tcatccaggc
     9301 gagttggccg gaggccatgc aggcgttcac gcccgagccg gtgaaacggt agtgccgctg
     9361 ataggcttcg gtcttcaggt aatcgtcgat gagcgcggcc gcgcgcgaat ccagccagag
     9421 caggccgccg ccgaccggct cgccgtcgga atcgatcatc caggcgccgt cgccctgggc
     9481 ggtgacggcg aggcccgcga cgcgctcgca gagattcggc gtccgctcga ccagttcgcg
     9541 aatcgtcgcc acgcaatcgg tccaggtccg cgccatatcc tgttcgacct ggcccggccc
     9601 gggcgtttca tagacattcg gtcgcgcggc ggcggcgatc tgttcgccgg caagcgaaaa
     9661 ggcgaccgac ttgatgaccg aagtgccggc gtcgacgccg atcaggatgt ccctggtcac
     9721 ggcggcggcg cctttcgaat cgccttttcg attgccggcc tcatgcagtc tctaatccat
     9781 gcgaaaccgc ggcggcggtc gcggcgtcaa agacgtgcag atcggcggcg ccgactccgt
     9841 agcggatcgc ctcgcctgct ttggctgcga tcggcttgtg cgaaaccgcg accaacagct
     9901 tgcccgcaac atccatcgcg aggtggcttt ggtcgccgag ccattggttg gagacgatcc
     9961 gcgcctcgtt cgcgccggcg ccgagcctta tcgcgtgcgg acgaatgccg atgacggccc
    10021 gatcgcgccc gccaatcgct tggcgaacat tcggcggtaa ttcggcggaa gcaaaatcga
    10081 atgtcgggcc atccttcacc gcgaaacgca tggattcgcg attctcgacg gcggcctcga
    10141 agacgttcat cggcggctcg ccgatgaagg ttgcgacgaa aagattggcg ggcttctcgc
    10201 gcaattcctt ctggctggcg aattgctgga gaagcccgcc ttccatcacc gcgatccggt
    10261 ccgccagcgc gttggcctcg gtctggtcgt gggtgacgaa aatcgaggtc atgcgccgct
    10321 cgaccagcag gctcttcagg cgggcgcgca gcaccgcccg caattgcggc tcgagttggc
    10381 ccatcggctc gtcgagaagg tagagatcgg cgcggcgcac gagcgcccgg gcgaggctgg
    10441 cgcgttgctg ctggccgcct gaaatcgacg aaggatagcg gtccaaaatc tcctcgattt
    10501 cgacaaggcg ggagatttct gcgacggact tctccacctc cttggcgttg agccgcgcgc
    10561 ttttcagcgc gaaggcgatg ttgtcgcgca cggtgagcgg cggatagagc gaatagccct
    10621 cgaaggccat ggcgacgccg cgccgcgccg gcggcaattg atcgatgcgg cgagcggcga
    10681 tggcgatatc gccgttcgag acggcttcga aaccggcgat catgcgcagc gtcgaggtct
    10741 tgccgcagcc ggacgagccg agcaatgcga cgatttcgcc gcgctcgacc gtcatgctta
    10801 aggccttcac ggcatggacc gggggcctgc cgcgccgctg gtagactttg tccacgttgt
    10861 tgagcgagag agcgatgtcg ggcatggatt tcaagccgat tgcggcgcga tgcggcgacc
    10921 gctcgcggcg tcgaaaagga gaatcgcgct cgggtcgaaa cgcgcatagg ccgggccgtg
    10981 gcggcgcggc gcctcgtcgg cgctcggctc cgaggcgaga agttcgcggc catcggcggt
    11041 ccgcagcaac aacaaggtct tttcgttgag aggcgtcgcg gcgaccaaat cgacatcgaa
    11101 gctttcaggc gagggatgac ttccgaggag aatcgattcc ggccgcagcc cgagcatgca
    11161 at
//
LOCUS       DATGJW010000494         8131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1340652, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000494 DATGJW010000000
VERSION     DATGJW010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8131
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1340652"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..254
                     /locus_tag="VKU03_14840"
     CDS             <1..254
                     /locus_tag="VKU03_14840"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013216815.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="chromate transporter"
                     /protein_id="HLJ72594.1"
                     /translation="GVLPFWTTLRQQPMVRAALRGVNASVVGLLIAALYHPVWTAGIV
                     NAADYAIAIAAFLLLFMWRTPPWLVVVLGALAGAALGAT"
     gene            complement(257..1513)
                     /locus_tag="VKU03_14845"
     CDS             complement(257..1513)
                     /locus_tag="VKU03_14845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6596 domain-containing protein"
                     /protein_id="HLJ72595.1"
                     /translation="MKGDENLAAYDAASAAARRSYGRLVAYLSARTRDVAGAEDALSE
                     AFAAALADWPKSGVPRNPEAWLLAAARRKSIDAARRRRTVAEASGHVQLLAEETAAMA
                     EESAPIPDERLSLMFACAHPAIEAAIRAPLILQTILGFDAATIASAFLVAPTTMGQRL
                     ARAKAKIRLAGIPFRTPEREDMADRLDAVLAAIYAAFAEGWTDPNGAEARRRNLAEEA
                     IWLGRLIVQLLPNEAEALGLLALMLYSEARRPARRDERGDFVPLDEQDMSRWDDRLID
                     EAEALLGSASAMKTIGRYQLEAALQSAHVVRRRGGGVDWSAIVTLYDALCALTGSPVA
                     AINRAVALAETQGAAAGLSALNAVAEDSRLAEYQPYWAARAELLTRTGAPGEARAAYD
                     QAIGLESDPAVRRFLQKKRALLRLHA"
     gene            complement(1524..1871)
                     /locus_tag="VKU03_14850"
     CDS             complement(1524..1871)
                     /locus_tag="VKU03_14850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008834979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YciI family protein"
                     /protein_id="HLJ72596.1"
                     /translation="MQYMLMIHSNENGFASLTKEQIEQAMGAYTSYTEALRKAGVLVS
                     SNRLQPVSAATTVRLADGKTQVLNGPYAETKEQIGGYYLIEASDLDGAISWASRCPGA
                     SHGTVEIRPVWPM"
     gene            2075..3277
                     /gene="pcaF"
                     /locus_tag="VKU03_14855"
     CDS             2075..3277
                     /gene="pcaF"
                     /locus_tag="VKU03_14855"
                     /EC_number="2.3.1.174"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA];
                     GO_process: GO:0019619 - 3,4-dihydroxybenzoate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoadipyl-CoA thiolase"
                     /protein_id="HLJ72597.1"
                     /translation="MIDVFLCEGARTPVGRYGGALAAVRPDDLLAHAIKATLAKAPKL
                     DAGAIDEVYAGCANQAGEDNRNVARMALLLADLPAEIPGATINRLCGSGLDAVGTAAR
                     AIRAGEIDLAIAGGVESMSRAPFVQGKATEAFSRQAEIFDTTIGWRFVNPLMKAQYGV
                     DSMPETAENVAEEFQISRADQDGFALRSQQRAAAAQKNGRLAREIAPVEIKDRKGNVT
                     KVERDEHPRETSLDKLAALATPFRKGGTVTAGNASGVNDGAAALIVASTEAVKRHGLT
                     PVARISGLATAGVAPRIMGYGPTPATQKLMARLKIAIGDFGVIELNEAFASQALACLR
                     ALGVPDDAEHVNPNGGAIAIGHPLGMSGARLALSAAIEMKERGAKRALATMCIGVGQG
                     IALGLEAV"
     gene            3299..4165
                     /locus_tag="VKU03_14860"
     CDS             3299..4165
                     /locus_tag="VKU03_14860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340667.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HLJ72598.1"
                     /translation="MARRKAPEPPGGRANLAEVKRFYGKLMAAAANSADPRLEDVFAQ
                     VPREAFMPPGPWRIMVNNRYFETPSADPAYLYQNALVALDPDKGVNNGEPFLHAGWIG
                     AVAPKPGETICHIGAGTGYYTAILAMLALPGGRVGAYEIDPDLAQKARDNLASFANVS
                     VMQADATEIPLPASDVIYVNAGVVAPPLSWLKALTSNGRMIFPWRPTNDIGLALVVRR
                     TNAGFAVKPLSSAWFIPCVGASDAEESVKAPSAAEAWAARSIWPLANRASDETAVAIY
                     REAWFSSAAPGD"
     gene            4343..5650
                     /locus_tag="VKU03_14865"
     CDS             4343..5650
                     /locus_tag="VKU03_14865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72599.1"
                     /translation="MTAIAIDPAMRRRVVWSSTIGNALEWFDFIVFGLFASVIGAQFF
                     PKSDATAQLLATYGLLGIAYFFRPIGGLVFGVWSDRIGRKRALIAIVIMMAIGTGLIG
                     VMPNYATIGVAAPLGLLIARLIQGFSAGGEYSTSTAMLVEFATPGRRGFYASFQHVAQ
                     FSAFVIGATFATLLNTFLSPEAFQSWGWRVPFLAGIIIGPVGAYLRTAVDETPEFKAF
                     LAKRASARNTPLRDVLRERPRELFALLLYVAGGTAFTYIMNIFLPTYAAKELGLKLTD
                     AQLGLLFVNIVAAIAIPFAGMVSDRYGRRPVMTPAIILFVIASLFFAEQMISAPSNAA
                     MWTLQAASLFMIFVTGPGNALMMETFPVHMRSTGAAIAYNLAVALFGGLSPWLVSNLI
                     AATGDKFMPFYYMDGCLILSLIGLAILPDPKGHAEEGTAASAR"
     gene            complement(5663..6550)
                     /locus_tag="VKU03_14870"
     CDS             complement(5663..6550)
                     /locus_tag="VKU03_14870"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HLJ72600.1"
                     /translation="MPRVLVVGDVMVDVIVRPEGPIAIGADRRATIRSKPGGSAANQA
                     AWLVHFGVAARLVGRVGAADKDAQENIFAKAGVEARLAPDETKETGRLVALIDPSGER
                     SFFTDRGANDGLVASDIPDALLDDIDHVHISGYSFVHPGPRGAMIDLIARAKRRDAPT
                     SVDPASSEFLREIGPANFLAWTRGVAMCFPNEDEARVLTDADAPSEQLARLAAHYPLV
                     VLKRGAAGCEAAEGAEGTRRWRATTPKVAAIDTTGAGDAFFAGFLAARLSGAPMEACL
                     QRAAQAGAKATLSLGGRPV"
     gene            complement(6550..7473)
                     /locus_tag="VKU03_14875"
     CDS             complement(6550..7473)
                     /locus_tag="VKU03_14875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014130251.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HLJ72601.1"
                     /translation="MTINRMLDIGADVAAAIAARRPVVALESTIITHGMPFPQNFETA
                     LKLEEIVRAQGAVPATIAVVDGRMVVGLDRAGLERLARLSGGVVKTSRRDLAAVLARG
                     GTGGTTVAATMYIAALAGVEIFATGGIGGVHRGAEETFDISADLVELSRSKVAVVCAG
                     AKSILDIPKTLEFLESQGVPVIGYRCDEFPAFYARSSGVKIDHRCEGLHDLARMIRLQ
                     SEIGPGGVLIANPIPEAHALDAAQIEARIEEAVREAKAQGVGQKDVTPFLLRRLYEMT
                     GGRSLEANMALVESNAALAAQVAGELARLSS"
     gene            7601..>8131
                     /locus_tag="VKU03_14880"
     CDS             7601..>8131
                     /locus_tag="VKU03_14880"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72602.1"
                     /translation="MEKTSETSRADDDPPRRTDWGRIGLLWLCGMGAGLQFAKASIGF
                     DALAAYYQASPALAGWLLSSVGAVGVVFGATAGLIVGRAGRRRSLVAALFGACLLSLI
                     QATLPSVGAFMTTRILEGATHLAIIVAAPVCLPDLTAPRHRAIVMALWGTFFNVAFLV
                     AGAVGPALLAGFGLKSL"
BASE COUNT         1363 a         2653 c         2599 g         1516 t
ORIGIN      
        1 tcggcgtctt gccgttctgg accacgctgc gtcaacaacc gatggtgcgc gccgcgctgc
       61 gcggcgtgaa cgccagcgtc gtgggccttc tcatagcagc gctctatcat ccggtctgga
      121 cggcagggat tgtgaacgcc gccgattatg cgatcgcaat cgccgccttc ttgctgcttt
      181 tcatgtggcg tacgccgcca tggctggttg tcgtgttggg agcattggct ggcgcggcgc
      241 tcggcgcgac ctaagcttaa gcgtgcaatc gtaacagcgc gcgcttcttc tgcagaaatc
      301 gccgcacggc gggatcagat tcaagaccga tcgcctgatc gtacgccgct ctcgcctcgc
      361 caggcgctcc ggtccttgtc agcagctccg ctcgcgccgc ccaataaggc tggtattcgg
      421 caagccttga atcctccgca acagcgttga gggcggataa acccgccgca gcgccttgcg
      481 tctcggcgag cgcgacggcg cggttgatcg cggcgacggg cgagcccgtc aaagcgcaaa
      541 gggcgtcata gagggtgacg atcgccgacc aatcgacgcc gccgccgcgc cgccgaacaa
      601 catgggccga ttgcagcgcc gcctccaatt gatagcggcc gattgttttc atcgcgctcg
      661 cactgccgag cagggcttcc gcttcgtcga tcaagcgatc gtcccaacgc gacatatcct
      721 gctcgtcgag gggcacaaaa tcgccgcgct cgtcgcgccg cgccggtctt cgcgcctcgg
      781 aatagagcat caaggcgaga aggccgaggg cttcggcctc gttcggcaaa agctgcacga
      841 taaggcggcc gagccagatc gcttcttccg caagattgcg ccgccgtgcc tcggcgccgt
      901 ttggatctgt ccatccttcc gcgaaggcgg cgtagattgc ggcgagcacg gcgtcgagcc
      961 tgtcggccat gtcctcgcgc tcaggggtac ggaagggaat gccggcgagc ctgatcttgg
     1021 ccttggcgcg cgccagcctt tgccccatcg tcgtcggcgc gacgagaaag gccgaggcga
     1081 tcgtcgccgc gtcgaagccg aggatcgtct gcaggatcag cggcgcgcgg atcgccgcct
     1141 cgatcgccgg atgcgcgcaa gcaaacatca ggctcaaacg ctcgtcggga atcggcgcgg
     1201 attcttcggc catcgccgct gtctcctcgg ccagcaattg gacatggccg ctcgcctcgg
     1261 cgacggtgcg ccgtcttcgc gccgcgtcga tcgatttccg ccgcgcggcg gcgagcagcc
     1321 aagcctcggg attgcgcggc acgccgctct tcggccaatc ggcgagcgcg gcggcgaaag
     1381 cctcggacaa agcgtcttcg gctcccgcga catcgcgcgt gcgcgcgctc aggtaagcga
     1441 cgagacggcc atagctacga cgcgccgcgg cggaagccgc gtcataagcc gccaggttct
     1501 catcgccttt cattcgcaga agattacatc ggccagacgg gcctgatctc cacggttccg
     1561 tgcgaagcgc cagggcatcg cgacgcccag gaaatcgcgc cgtcgagatc cgaagcctcg
     1621 attagataat agccgccgat ctgctccttc gtctccgcat aggggccgtt gagaacctgg
     1681 gtcttgccat cggcgaggcg caccgtcgtc gccgccgaga ccggctgcag acgattgctc
     1741 gagaccagca cgccggcctt tctcagggct tccgtatagg aggtgtaagc gcccatggcc
     1801 tgttcgatct gctctttggt cagcgaagca aagccattct cgttggaatg gatcatcagc
     1861 atgtattgca tcggaattct cctcatctcg tcagcgccgc gagggttgcg ccgctgaacc
     1921 catgacgcgc gacgatgccc cgtttcgaca atggcgggca aaaattagca ggccctgccc
     1981 ttatgtcatt cccgcttcga cgggaatgac atagcgcgcc cgatggcgga acgactatat
     2041 tcacggcgac gtttcacatt ccggagccgc ctccatgatc gatgttttcc tttgcgaggg
     2101 cgcacgcacg ccggtcgggc gctatggcgg cgcgcttgct gcggtcaggc ctgacgatct
     2161 cctcgctcat gcgatcaagg cgacgctcgc caaggcgccg aagctcgacg cgggcgccat
     2221 cgacgaagtc tacgccggct gcgccaatca ggccggcgag gacaatcgca atgtggcgcg
     2281 gatggccctg ctgctcgcgg acctgccggc ggaaattccg ggcgcgacga tcaaccgcct
     2341 ctgcggctcg ggccttgacg cggtcggcac tgcggcgcgc gccattcgcg ccggcgagat
     2401 cgatctggca atcgcgggcg gcgtcgaatc catgtcgcgc gccccttttg tccagggcaa
     2461 ggcgacggaa gcgttttcgc gccaagcgga aatcttcgat acgacgatcg gctggcgctt
     2521 cgtcaatccg ttgatgaaag cgcaatatgg cgtcgattcg atgccggaga cggcggagaa
     2581 tgtcgccgag gagtttcaga tttcccgggc ggaccaggat ggtttcgcgc tgcgcagcca
     2641 gcaacgcgcc gccgccgcgc agaagaatgg ccgcctcgcg cgcgagatcg cgcccgtcga
     2701 gatcaaggac cgcaaaggca atgtgaccaa ggtcgagcgc gacgaacatc cgcgcgagac
     2761 aagcctcgat aagcttgccg cgctggcgac gcccttccgc aaaggcggca ccgtcaccgc
     2821 gggcaacgct tccggcgtca atgacggcgc cgcggcgctg atcgtcgcct cgacggaagc
     2881 ggtgaaacgc catgggctga cgcctgtcgc tcgcatatcg ggccttgcga ccgcaggcgt
     2941 cgccccgcgc attatgggtt acggcccgac gccggcgacg cagaagctga tggcgcggct
     3001 caaaatcgcg atcggcgatt tcggcgtcat cgaactcaac gaagctttcg cctcgcaggc
     3061 gctcgcctgc cttcgcgcgc tgggcgtgcc cgacgacgcc gagcacgtca atccgaatgg
     3121 cggcgcgatc gcgattggcc atccgctcgg catgtcgggc gcgcggcttg cgctcagcgc
     3181 ggccatcgaa atgaaggagc gcggcgccaa acgggccttg gcgaccatgt gcatcggcgt
     3241 tgggcagggc atcgcgcttg gactcgaggc ggtttagcgc ttggaaacag gaggcgcgat
     3301 ggctcgtcga aaggcgcccg aaccgccagg aggccgcgcc aacctcgcag aggtcaagcg
     3361 tttctacggg aaactgatgg ccgcggcggc aaattccgcc gacccgcggc tcgaagacgt
     3421 cttcgcgcaa gtcccgcgcg aagccttcat gccgccggga ccttggcgga tcatggtcaa
     3481 taaccgctat ttcgaaacgc caagcgccga ccccgcctac ctctatcaga acgcgctggt
     3541 cgcgctcgat cccgacaagg gcgtcaacaa cggcgagccg ttccttcatg ccggttggat
     3601 cggcgcggtc gcgccaaagc ccggagagac gatctgtcat atcggcgcag gcacagggta
     3661 ctataccgct attctcgcaa tgctggcgct tccgggcggt cgcgtcggag cgtacgagat
     3721 cgatcccgac cttgcgcaaa aggcgcggga caatctcgcc tccttcgcaa atgtgtcggt
     3781 catgcaggcc gacgcgacag agatcccgct cccggcgtcg gatgtgatct atgtcaacgc
     3841 cggcgtcgtc gcgccgccgc tgtcctggct caaggcgttg acgtcaaatg ggcgcatgat
     3901 ctttccttgg cggccgacga acgacatcgg tctcgcgctc gtcgtgaggc gaacgaacgc
     3961 gggtttcgcc gtgaagccgc tatcgtcggc gtggttcatt ccgtgcgtcg gcgcgtcgga
     4021 cgccgaggag agcgtcaaag cgccgagcgc ggcggaagcc tgggccgcgc gttcgatctg
     4081 gccgcttgcg aatcgcgcgt cagacgaaac ggcggtcgcg atttatcggg aggcgtggtt
     4141 ctcgtccgcc gcgccggggg actaacaagc gccgccgatc gcttccgcat cggcgctcgt
     4201 gctgaagtac gatcgcctga caaaaattcg gcgcgcgcgg cgcatttgac acagatcgtt
     4261 tgatcctcaa cattctgcgc gacggcgcgg aacgcctggg gagggtcttg ttcgcacgac
     4321 cgattcggca aggggggttg gcatgacggc gatcgcgatc gatcccgcca tgcgacggcg
     4381 cgtcgtgtgg tcgtcgacga tcggcaatgc gctcgaatgg ttcgatttca ttgtcttcgg
     4441 cctattcgcc agcgtgatcg gcgcgcaatt ttttcccaag agcgatgcga ccgcgcagct
     4501 tctcgcgacc tacggccttt tggggatcgc ttatttcttt cggccgatcg gcggccttgt
     4561 cttcggcgtt tggtcggatc gaatcggacg taaacgggcg ctgatcgcca tcgttatcat
     4621 gatggcgatc ggcaccggcc tcattggcgt gatgccgaat tatgcgacga tcggcgtcgc
     4681 cgcgccgctc ggcctgctca tcgcccgtct aattcaaggg ttctccgcag gcggcgaata
     4741 ttcgacgtcg acggctatgc tggtggaatt tgcgacgcct ggacggcgcg gcttctacgc
     4801 cagttttcaa catgtcgcgc aattctccgc tttcgtgatc ggcgcaactt tcgcgacctt
     4861 gctgaacact tttctttcgc cggaagcctt ccagagctgg ggctggcgcg ttcctttcct
     4921 tgcgggaatc atcatcggcc cggtcggcgc ctatcttcgc acggccgtcg atgagacgcc
     4981 ggaattcaaa gcattcctcg ccaaacgcgc cagcgcgcgc aatacgccgc tccgtgacgt
     5041 cttgcgagag cggccgcgtg aactcttcgc cttgctgctc tatgtcgctg gcggaaccgc
     5101 cttcacctat atcatgaaca tttttctgcc gacctatgct gcgaaagagc tcggtctcaa
     5161 actgactgac gcgcagcttg ggcttctctt cgtcaatatc gtcgctgcga tcgccattcc
     5221 cttcgctggc atggtttccg atcggtacgg aaggcgtccc gtgatgacgc cggcgatcat
     5281 tctgttcgtg atcgcttcgc ttttcttcgc cgagcaaatg atcagcgcgc cgagcaacgc
     5341 ggcgatgtgg actttgcaag cagcgagcct cttcatgatt ttcgtgacgg gtcccggcaa
     5401 tgcgctgatg atggagactt tcccggtcca tatgcgctcg accggcgcag cgatcgctta
     5461 caatctcgcc gtcgcgctct ttggaggtct ctcgccctgg ttagtgagca atctcatcgc
     5521 cgcgaccggg gacaagttca tgccgttcta ttatatggac ggctgtctca tcctgtcgtt
     5581 gatcgggctc gcgatcctgc cggatccgaa gggacacgcc gaagagggta cggcggcgag
     5641 cgcgcgataa ccggatgcta aatcagaccg gccttccgcc aagcgacaaa gtcgctttcg
     5701 ctcccgcttg cgccgcgcgt tgcagacacg cctccatcgg cgcgcccgac aatcgcgcgg
     5761 cgagaaatcc cgcgaaaaag gcgtcgcccg cgccggtcgt gtcgatcgcg gcgactttcg
     5821 gcgtcgtcgc ccgccagcgc cgcgtccctt cggcgccttc cgcggcctcg catccggccg
     5881 cgccgcgttt caggaccacc aagggataat gcgcggcgag acgcgcgagt tgctcgcttg
     5941 gcgcatcggc gtcggtgagt acgcgcgcct cgtcctcatt cgggaaacac atggcgacgc
     6001 cgcgcgtcca ggcgagaaag ttcgccggcc cgatctcgcg aaggaattcg ctcgaggcgg
     6061 gatcgaccga cgtcggcgcg tcgcgccttt tggcgcgcgc gatgagatcg atcatggcgc
     6121 cgcgggggcc gggatggacg aaggaatagc ctgaaatatg gacgtgatcg atatcgtcga
     6181 gcagcgcgtc gggaatatcg ctcgcgacaa gaccgtcgtt ggcgccgcga tcggtgaaga
     6241 aactgcgctc gccggacggg tcgatcaagg cgacgagccg gcccgtctct ttcgtctcgt
     6301 cgggcgcgag acgcgcttcg acgccggcct tggcgaagat attctcctgc gcgtccttgt
     6361 ctgcggcgcc gacacggccg acgagccgcg ccgcaacgcc gaagtggacg agccaggccg
     6421 cctgattggc ggccgagccg cccggctttg agcgaatcgt cgcgcgccgg tcggcgccga
     6481 tggcgatcgg cccttcggga cgtacgatca cgtcgaccat gacgtcgccg acaacgagga
     6541 cgcgcggcat cagctcgaca gtcgcgccaa ttcgcccgca acttgcgccg cgagcgccgc
     6601 gttggattcg accagcgcca tatttgcttc gaggctgcgc ccgccggtca tttcgtagag
     6661 acgacgcagg aggaagggcg tgacgtcctt ctgacctacc ccttgcgcct tggcctcgcg
     6721 cacggcttct tcgatccgcg cctcgatctg cgccgcgtcg agcgcatggg cttcagggat
     6781 cggattggcg atcagcacgc cgcccggccc gatttcgctc tggaggcgga tcatgcgggc
     6841 gagatcgtgg aggccttcgc agcgatggtc aattttgacg ccgctcgaac gcgcgtagaa
     6901 ggcgggaaat tcatcgcagc gatagccgat gaccggcacg ccttgcgatt cgagaaattc
     6961 caaggttttc ggaatatcga gaatggattt ggcgccggcg cagaccaccg cgactttgct
     7021 tcgcgagagt tcgacgaggt cggcggaaat atcgaacgtt tcctccgcgc cgcggtggac
     7081 gccgccaatg ccgccggtcg caaaaatttc gacgccggcg agcgcggcga tatacatcgt
     7141 cgccgcgacc gtcgtgccgc ccgtgccgcc ccgcgcaaga accgccgcaa gatcgcgccg
     7201 cgacgtcttg acgacgccgc cggagagacg ggcgagcctt tccaagccgg ctcggtcgag
     7261 gccaacgacc atgcgtccgt cgacgactgc gatggtcgct gggaccgctc cttgcgcccg
     7321 cacaatctct tcgagtttca acgccgtctc gaaattctgc ggaaagggca tgccatgcgt
     7381 gatgatggtc gattcaagcg ccaccaccgg gcgccgcgcc gcaatcgccg ccgccacgtc
     7441 cgcgccaatg tcgagcatgc ggttgatcgt catgaataaa aggctccctt aaatgcgcgc
     7501 aaccaatggc ctctccatat cagtttcagg ccgcacgaac caaggcgcgc ctgcgcgttg
     7561 atagacgcga ccgccgcgca aggttagaac gccacgcttc atggaaaaga ccagcgaaac
     7621 gtcccgcgcc gatgacgatc cgccgcgccg caccgactgg ggccggatcg ggctgttatg
     7681 gctatgcggc atgggcgccg gcttgcaatt cgccaaggcc tctatcggtt tcgacgcgct
     7741 ggctgcttat tatcaggcct cgccggcgct tgccggctgg ctgctctcgt ccgtcggggc
     7801 ggtcggcgtc gtttttggcg ccacggccgg gctgatcgtt ggccgcgccg ggcggcggcg
     7861 cagtcttgtc gcggcgcttt tcggggcctg ccttctttcg ctgattcaag cgacgctgcc
     7921 gtccgtcggc gcattcatga cgacgcgtat tttggaaggc gcgacccatc ttgcgatcat
     7981 cgtcgccgcg ccggtgtgtt tgccggacct gaccgcgcct cgtcaccgcg cgatcgtgat
     8041 ggcgctttgg gggacattct tcaatgtcgc cttcctcgtg gccggcgccg tcgggccggc
     8101 cctcctggcc ggtttcggct tgaagagcct c
//
LOCUS       DATGJW010000495         8738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_924257, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000495 DATGJW010000000
VERSION     DATGJW010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8738
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_924257"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..828)
                     /locus_tag="VKU03_14885"
     CDS             complement(100..828)
                     /locus_tag="VKU03_14885"
                     /EC_number="2.1.1.130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018264330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030788 - precorrin-2
                     C20-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-2 C(20)-methyltransferase"
                     /protein_id="HLJ72603.1"
                     /translation="MKKGRLIGVGLGPGDPELLTVKAVETIRSSPVIAFFAKAGRRGN
                     ARQIADRWIPKESIELPLYYPMTTESRFDGPVYVEALRAFYEEAAAALAGHLDQGRDV
                     ALLAEGDPLFYGSFMHLYMRLRERFATAIVPGVTGMSGCWGAAGAPIAWGDDVMTVLP
                     GTLPEEELVARLANTHAAVVLKLGSNFAKARAAIVKAGLGERAIYVERGTMLGERVMR
                     LADKLDDKAPYFSLILIPGEGRRP"
     gene            complement(825..1454)
                     /locus_tag="VKU03_14890"
     CDS             complement(825..1454)
                     /locus_tag="VKU03_14890"
                     /EC_number="5.4.99.61"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016993 - precorrin-8X methylmutase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-8X methylmutase"
                     /protein_id="HLJ72604.1"
                     /translation="MNRYSYIRDGAEIYRRSFAIIRAEADLARFTQEQEPVAVRIIHA
                     CGQVEAANDLVFSDTFVSAAKAALDKGAPILCDSKMVADGVTRARLPAKNEVICTLAD
                     PATAEIAARIGNTRSAAALDLWRDRIAGSLVVIGNAPTALFRLLELLDEGWPKPAAAI
                     GMPVGFVGAAESKAALIADRRIPYLVAAGRRGGSAMAAAAVNALASAKE"
     gene            complement(1451..2791)
                     /gene="cobG"
                     /locus_tag="VKU03_14895"
     CDS             complement(1451..2791)
                     /gene="cobG"
                     /locus_tag="VKU03_14895"
                     /EC_number="1.14.13.83"
                     /inference="COORDINATES: protein motif:HMM:TIGR02435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016709 - oxidoreductase activity, acting
                     on paired donors, with incorporation or reduction of
                     molecular oxygen, NAD(P)H as one donor, and incorporation
                     of one atom of oxygen [Evidence IEA];
                     GO_process: GO:0019250 - aerobic cobalamin biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="precorrin-3B synthase"
                     /protein_id="HLJ72605.1"
                     /translation="MSAAAARQSEREALRRGWCPGALRPMASGDGLIARLRVVGGALA
                     PKAARAIAEAAASLGNGQIDLTSRANLQIRGVDERTLPALQAAIARLGLTDANPDAEA
                     VRNIVASPLAGLDPAALLDIRPPVAALDRRLRQDRALWLLPGKFAFLVDDGGSLSVAG
                     EPADIAFAAQRRGGEVVFALRLAGQPAGRCAVAEAADVAARLSEAFLALRGRNESAAR
                     RMRTLVERIGMAPILRAAGLNADAPTVPEAPRAAPLLLGLRDLGRFRALGVGAPFGRL
                     DATQLTALADAAESAEGALRLTPWRAVLIAGKRVDEALALRLREQGFVLDEDDPIRAV
                     AACPGAPACANASTETQKDAARLAPLARRLASHGVALHVSGCAKGCARAAATATTFVG
                     RSGWYDIVLDGCACDEPALLGVAIENLSAALEALAGAPRGQRAAALRAFAMERP"
     gene            complement(2788..6117)
                     /gene="cobN"
                     /locus_tag="VKU03_14900"
     CDS             complement(2788..6117)
                     /gene="cobN"
                     /locus_tag="VKU03_14900"
                     /EC_number="6.6.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173668.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobaltochelatase subunit CobN"
                     /protein_id="HLJ72606.1"
                     /translation="MHLLRTTTRLIEEAEAAVDLGQSPGDIVFLSFSDSDLGVVAAAL
                     ENETPASPRARLASLAMLRHPYSVDLYIDKVAAKARFVLVRLLGGLDYWRYGALELAA
                     AARRGGFDLAIVPGDGRPDPRLAELSTLAAEETEAIWRAFEAGGLGNIARLFGWIGRR
                     IGGDAAPPPAPERVPAYGLYAAACRPGTPDQALAAIVFYRAYLLAGDLAPIVALADAL
                     AARGARVLAAYAPSLKDAEAAAWLADLLRREQPDVVVNSTAFAARMERETSPLEAADA
                     PIIQAIHAGASRAAWARDPRGLVGADLAMNVVLPELDGRIIARTISFKDEAPRSEALQ
                     FSRLVHQPEPSRVAFVAELALAWARLRRLPRGERRLACIVSDYPAKAGRAGYAVGLDT
                     PASLAAIAARLKAEGYSIEAIADADEFFRTLAVAPLNEQFALADYEAEFARLPPAFRE
                     SVAAAWGEPERDLDCRDGGFHFRVAQLGAMIVALQPDRGGRAARKSEYHDPRLPPRHG
                     YVAFYLWLRRQRIDAMIQLGAHGTLEWLPGKAVALSEDCAPEALLGPTPLIYPFIVNN
                     PGEAAQAKRRAGAVIVSHLTPPLAEAGASGPLLEFESLFDEYAAAQQMDAKRAALLAK
                     TILERAAETGLAAESGAAADASDEDRLARLDAWLCDIKEMRIADGLHIFGSAPSGDVL
                     AGAEADDAARARLAACPESEMAGLLAALDGRFVPPGAAGAPGRGRLDVLPTGRNLFTI
                     DPRTIPTRTAWTIGQRAAEALVARHLQDHGDWPRRLVVDLWGSATMRTGGEELAQIFA
                     YLGATPVWDHGSGRVTGFTILPSAALPRPRIDVTLRLSGLFRDVFPAQIALLEQLIGA
                     VAGLDEPAEFNPLAAARAMGESPLRLFGPAPGAYGAEIGRLLADDPDADVQTLAEAYL
                     TASGYALGAADREARDLFRARVAEADVYVHIQDLPGQDALEASAFFEHEGGFAAAAKS
                     LGGKASLYHLDATRSERPAVRTLREEIARAVRGRAANPRWIAGQMRHGYRGGGEIAET
                     VDNLYGFAVSSGAVTPQLFDLVFEATLGDDAVAAFLAEANPQAARAIAARFDQALRRG
                     LWTCRRNSTAMRLAAFLERAA"
     gene            complement(6126..7163)
                     /gene="cobW"
                     /locus_tag="VKU03_14905"
     CDS             complement(6126..7163)
                     /gene="cobW"
                     /locus_tag="VKU03_14905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592245.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin biosynthesis protein CobW"
                     /protein_id="HLJ72607.1"
                     /translation="MASPDVSKIPTTIVTGFLGAGKTTLIRHLLENARGRRLALLINE
                     FGDVGVDGEILRGCGVETCPEENIIELSNGCICCTVADDFIPAIETLLNRPQKPDHIV
                     IETSGLALPKPLVKAFDWPSIRSRLTVDGVVTVIDGAAVAAGRFADDPAAVAAQRQSD
                     MALDHDNPLEEVFEDQLLCADMIVLNKTDLMSEAERDATRAKIASVLPRAVKIVATSE
                     GRIDAEVLLGLEAAAEDDVDSRKSHHDGEEEHDHDEFETFVVAVGPLADPDRFIARLA
                     QIAERHDVLRMKGFFAVDGKPMRLLVQGVGKRFRQHFDRAWRPGEERAGRLVVIGEAG
                     LDRAAIAAAIA"
     regulatory      complement(7276..7475)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00174"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="cobalamin riboswitch; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="adenosylcobalamin"
                     /db_xref="RFAM:RF00174"
     gene            7540..>8738
                     /locus_tag="VKU03_14910"
     CDS             7540..>8738
                     /locus_tag="VKU03_14910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="nodulation protein NfeD"
                     /protein_id="HLJ72608.1"
                     /translation="MTRLRSGLVFAAVALAALALPTATAAAAERALLLDIDGPIGPPI
                     AEYIVRELGAAKLGEVGLVILRMNTPGGLDASMRRIISAILASPVPVATYVAPNGARA
                     ASAGTYIAYASAIAAMAPSTNIGAATPVQLGGNPLAPEQKPQKEQKQGEGGSQDVETR
                     KIVNDAVAYIRSLAALNGRNADWAAEAVRSAASLPASEALSLHVIDTIADNVPDLLRK
                     IDGRTVTVDGKPQRLATAGLEVVAAPPGWRTELLALITNPNVAFILMLIGVYGLILEF
                     FNPGAVAPGLIGAISLLVALYALALLPINYAGAALVLLGVALMVAEAHIGAFGALGVG
                     GVAAFVVGALMMFPARAPGFALSGGVIAGAAIGSAALLLLILAALLRSRKQPVVTGGE
                     ALIGAEG"
BASE COUNT         1363 a         2971 c         3092 g         1312 t
ORIGIN      
        1 ccgatctgtc ggtcgccgct tcgatcagcg gctgggtctc gagcgtcatc cactcgcgcg
       61 gacccggtcc gagcccgaca atgtcgaggc gtcccgcgct cacggccgtc gcccttcccc
      121 tggaatgaga atcagcgaaa aatacggggc tttgtcgtcg agcttgtcgg cgagccgcat
      181 gacgcgttcg ccgagcatgg ttccccgttc gacataaatg gcgcgctcgc cgagccccgc
      241 tttcacgatc gcggcgcgcg ccttcgcgaa attgctgccg agcttcagca ccacggcggc
      301 gtgggtattg gcgaggcggg cgaccagctc ctcttcaggc agcgtccctg gaagcacagt
      361 catcacgtcg tcgccccagg cgatcggcgc gccggccgcg ccccagcagc cggacattcc
      421 ggtgacgccc ggtacgatcg ccgtcgcgaa gcgctcgcgg agtcgcatgt agagatgcat
      481 gaacgagccg tagaagagcg ggtcgccttc ggcgagcagc gcgacgtcgc ggccctgatc
      541 gagatggccg gcgagcgccg ctgccgcctc ttcatagaag gcgcgcaacg cctccacata
      601 gactgggccg tcgaaacgcg actcggtcgt catcggataa tagagcggca attcgatcga
      661 ctctttggga atccagcggt cggcgatttg ccgcgcattg ccgcgccgcc cagccttggc
      721 gaaaaacgcg atcaccggcg acgagcggat ggtctcgacc gccttgacgg tgaggagttc
      781 gggatcgccg ggtcccagcc cgacgccgat caatctgccc tttttcattc tttggcgctc
      841 gccaaggcgt tgacagccgc ggcggccatc gcgctgccgc cgcgccggcc ggccgcgacg
      901 aggtacggaa tgcgccggtc ggcgatcaaa gccgctttcg attcggccgc gccgacaaag
      961 ccgaccggca tgccgatcgc ggcggccggc ttcggccagc cttcgtcgag aagctccaga
     1021 agccggaaca aggcggtcgg cgcgttgccg atcacgacca ggctgccggc aatgcgatcg
     1081 cgccagagat cgagcgcggc cgccgagcgg gtattgccaa ttcgcgcggc gatctcggcc
     1141 gtggcgggat cggccaaagt gcaaatcact tcattcttgg cgggcagccg cgcgcgggtg
     1201 acgccgtcgg cgaccatttt cgaatcgcag agaatcggcg cgcccttgtc gagcgcggcc
     1261 ttcgcggcgg agacgaatgt gtccgaaaaa acaaggtcgt tcgccgcctc gacctggccg
     1321 caagcgtgaa tgatgcgcac cgcgaccggc tcctgttcct gggtaaagcg cgccaaatcg
     1381 gcctcggcgc gaatgatcgc gaaagagcgg cggtagattt ccgcgccgtc gcggatatag
     1441 gaatagcggt tcacggtcgc tccatggcga aagcgcggag cgcggccgcg cgctggccgc
     1501 gcggcgcgcc ggcgagcgct tccagagcgg cgctgagatt ctcgatggcg acgccaagga
     1561 gggccggttc atcgcaggcg catccatcga ggacgatatc gtaccatccg ctccgcccga
     1621 cgaaggtcgt cgccgtcgct gcggcgcggg cgcagccctt ggcgcagccc gagacatgca
     1681 gcgcgacgcc atgcgacgcc aatcggcgcg caagcggcgc caagcgcgcg gcgtcttttt
     1741 gcgtctcggt cgaagcgttg gcgcaggccg gggcgccggg acaggcggcg acggcgcgga
     1801 tcggatcgtc ctcatccaga acgaatccct gttcgcgcag gcgcagcgcg agcgcttcgt
     1861 cgacccgctt gccggcgatc aggaccgccc gccacggggt caggcgcaac gcgccttcag
     1921 ccgattcggc ggcgtcggcg agggcggtca actgcgtcgc gtcgaggcgt ccgaacggcg
     1981 cgccgacgcc gagcgcgcga aagcgcccaa gatcgcgcag gccgaggagg agcggggcgg
     2041 cgcgcggcgc ctccggtacg gtcggagcgt cggcgttcag gccggccgcg cggaggatcg
     2101 gcgccatgcc gattcgctca accaacgtcc gcattcgccg cgcggcgctt tcattgcggc
     2161 cgcgcaaagc gagaaaagcc tcgctgagcc gcgccgcgac atcggcggcc tccgcgaccg
     2221 cgcaacgtcc cgccggctgg ccggcgaggc gaagcgcgaa tacgacttcg ccgccgcgtc
     2281 tttgcgcggc gaaggcgata tccgccggct cgccggcgac cgacagcgag ccgccgtcgt
     2341 cgacgaggaa tgcgaacttg cccggcagaa gccaaagcgc gcgatcctgc cgcaaccgcc
     2401 ggtcgagcgc cgcgaccggc ggccgaatgt cgagcagtgc ggcgggatcg agcccggcga
     2461 gcgggctcgc gacgatgttg cggaccgcct cagcgtcggg attggcgtcg gtcaggccga
     2521 gccttgcgat ggcggcctgc aaggcgggga gcgtgcgctc gtcgacgccc cggatctgca
     2581 gattggcgcg gctggtgaga tcgatctgac cgttgccgag agacgcggcg gcttcggcga
     2641 tggcgcgcgc agctttcggc gcgagcgcgc cgccgacgac gcgcaggcgc gcgatcaagc
     2701 cgtcgccgct cgccatcggc cgcagcgcgc cggggcacca gccgcgccgc agcgcctctc
     2761 tttcgctttg tcgagcggcg gcggcgctca tgcagcgcgc tccaggaaag ccgccaagcg
     2821 catcgccgtc gaattgcggc ggcaggtcca aagcccccgc cgcagggcct ggtcgaaccg
     2881 cgctgcgatc gcccgcgcgg cttggggatt ggcctcggcg agaaaggcgg cgacggcgtc
     2941 gtcgccgagc gtcgcctcga aaaccaggtc gaaaagttgt ggcgtcacgg cgccgctgct
     3001 gacggcgaaa ccatagaggt tgtcgaccgt ttcggcgatc tcgccgccgc cgcgatagcc
     3061 gtgccgcatc tggccggcga tccagcgcgg gttggccgcg cggccgcgca cggcgcgggc
     3121 gatctcctcg cgcagagtcc gtacagccgg cctctcgctg cgcgtcgcgt cgagatgata
     3181 gagactggcc ttgccgccga ggcttttcgc cgccgcggcg aagccgccct catgctcgaa
     3241 aaacgcgctg gcttcgagcg cgtcctggcc gggcaaatcc tggatatgga catagacgtc
     3301 ggcctcggcg acgcgcgcgc ggaaaagatc gcgcgcctcg cgatccgccg cgccgagcgc
     3361 atagccgctc gcggtcaggt aggcctcggc gagcgtttgg acgtcggcgt cgggatcgtc
     3421 cgcaagaagg cggccgatct ccgcgccata ggcgccgggc gccgggccga aaagccggag
     3481 cgggctttct cccatggcgc gcgcggcggc gagcgggttg aactccgccg gctcgtccaa
     3541 gccggcgact gcgccgatca attgttcgag cagcgcgatc tgcgccggaa agacgtcgcg
     3601 gaacaggccg gagagccgca aagtcacgtc gatgcgcggc cgcggcagag cggcggaggg
     3661 aagaatcgtg aatccggtca cccggcccga accatggtcc cagaccggcg tcgcgccgag
     3721 ataggcgaaa atctgcgcca gctcctcgcc accggtgcgc attgtcgccg agccccagag
     3781 gtcgacgacg aggcggcgcg gccagtcgcc atggtcctgc aggtgacggg cgacgagcgc
     3841 ctcggcggcg cgctggccga tcgtccaggc cgtgcgcgtc ggaatcgtgc gcggatcgat
     3901 ggtgaagaga ttgcggccgg tcgggagaac gtcgagccgg ccgcggccgg gcgcgcccgc
     3961 ggcgccgggc ggaacgaagc gcccgtccaa tgcggcgagg aggccggcca tttcgctttc
     4021 cgggcacgcg gcgaggcggg cgcgcgccgc gtcgtccgcc tcggcgccgg caaggacgtc
     4081 gcccgacggc gccgagccga atatgtgcag gccgtcggcg atgcgcatct ccttgatgtc
     4141 gcaaagccag gcgtcgagcc gcgcgagcct gtcctcgtcg gaggcgtcag ccgccgcgcc
     4201 gctctcggcg gcgaggccgg tttccgctgc gcgctcgagg atcgtcttgg cgaggagcgc
     4261 ggcgcgcttg gcgtccatct gctgggcggc ggcgtattcg tcgaacaggg attcgaattc
     4321 gagcagcggc ccgctcgcgc cggcctcggc cagcggcggc gtcagatggc tgacgatcac
     4381 ggcgccggcg cggcgcttgg cctgggcggc ctcgccggga ttgttgacga tgaacggata
     4441 gatcagcggc gtcgggccga gcagcgcctc cggcgcgcag tcttcgctga gcgcgacggc
     4501 cttgcccggc agccattcga gcgtaccatg cgcgccgagc tggatcatcg catcgatccg
     4561 ttggcggcgc agccaaaggt agaaggcgac atagccatgg cgcggcggca ggcgcggatc
     4621 gtgatactcg ctcttgcgcg cggcgcggcc gccgcgatcc ggctggagcg cgacgatcat
     4681 cgcgccgagc tgcgcgacgc ggaaatggaa gcctccatcg cggcaatcaa ggtcgcgctc
     4741 aggctcgccc caggctgcgg cgacggattc gcggaaagcc ggcggcaagc gcgcgaattc
     4801 cgcctcatag tcggcgagcg cgaattgctc gttcaaagga gcaacggcaa gggtacgaaa
     4861 gaactcgtcg gcgtcggcga tcgcctcgat ggaatagccc tccgccttca gccgcgccgc
     4921 gatcgcggcg aggctcgccg gcgtatcgag gccgacggcg tagccggcgc ggcccgcctt
     4981 ggcgggatag tccgagacaa tgcaggcgag cctgcgctcg ccgcgcggca ggcggcgcag
     5041 ccgcgcccag gccaaagcaa gctcggcgac gaaagcgacg cgcgacggct ccggctggtg
     5101 gacaaggcgc gagaactgca aagcctcgct gcgcggcgct tcgtccttga acgaaatcgt
     5161 ccgcgcgatg atccggccgt cgagttcggg caggacgaca ttcatcgcga gatcggcgcc
     5221 gacgaggccg cgcggatcgc gcgcccaagc ggcgcgcgaa gcgccggcat ggatcgcttg
     5281 tatgatcggc gcgtccgccg cttccagcgg gctcgtctcg cgctccatgc gcgcggcgaa
     5341 agccgtgctg ttgacgacga cgtcgggctg ctcgcgccga agcagatcgg cgagccaggc
     5401 cgcggcttcg gcgtccttca ggcttggcgc ataagccgcg agcacgcgcg cgccgcgcgc
     5461 cgccagggcg tcggcgagcg cgacgatcgg cgcaagatcg ccggcgagca gataggcgcg
     5521 gtagaagacg atcgccgcca gcgcctggtc tggcgtaccg ggccggcagg ctgcggcgta
     5581 gaggccgtag gccgggacgc gctcgggcgc cgggggcggg gcagcgtcgc cgccgatgcg
     5641 ccggccgatc cagccgaaca ggcgggcgat gttgccgagg ccgccggctt cgaaggcgcg
     5701 ccaaatcgcc tccgtctctt cggcggcaag ggtcgagagt tcggcgaggc gcgggtcggg
     5761 ccgcccgtcg ccgggaacga tggcgagatc gaacccgccg cgccgcgccg cggcggcgag
     5821 ctccaacgcg ccatagcgcc agtaatcgag tccgccgagc agccgaacca ggacgaagcg
     5881 cgccttcgcc gcgaccttgt cgatatagag atcgaccgaa tagggatggc gcagcatcgc
     5941 cagcgaggcg agccgcgcgc gcggcgaagc gggcgtttcg ttttcaagcg ccgcggcgac
     6001 cacgccgaga tcgctatcgg aaaacgacag gaacacgatg tcgccgggcg actggccgag
     6061 atcgacggcg gcttccgcct cctcgatcaa tcgcgtcgtc gtgcgcaaca ggtgcatggc
     6121 ggcgatcagg cgatcgcggc ggcgatggcg gcgcggtcga gcccggcttc gccgatcacc
     6181 accaggcggc cggcgcgctc ttcgcccggc cgccaggcgc ggtcgaaatg ctggcggaat
     6241 cgcttgccga cgccttgcac cagtaagcgc atcggcttgc cgtcgacggc gaagaagcct
     6301 ttcatccgca gcacgtcgtg gcgctcggcg atttgcgcga gcctggcgat gaaacgatcc
     6361 gggtcggcaa gcggcccgac cgcgacgacg aatgtctcga attcgtcgtg atcgtgctcc
     6421 tcctcgccat catgatgaga tttgcgcgaa tcgacgtcgt cctcagcggc cgcttcgagg
     6481 ccgagcagca cttcggcgtc gatccgcccc tcgctcgtcg ccacgatctt gaccgcgcgc
     6541 ggcagcacgg aagcgatctt ggcccgcgtc gcgtcgcgct ccgcttccga catcaggtcg
     6601 gtcttgttga gcacgatcat gtccgcgcag agcaattgat cctcgaagac ctcttcgagc
     6661 ggattgtcgt gatcgagcgc catatcgctt tggcgctgcg cggcgactgc ggccggatcg
     6721 tcggcgaatc gtccggccgc caccgcggcg ccgtcgatca ccgtcacgac gccgtcgacg
     6781 gtcaggcgcg agcggatcga cggccagtcg aacgctttca ccagcggctt cggcagggcg
     6841 aggccggatg tctcgatgac gatgtggtcg ggcttttgcg gccggttcag cagggtttcg
     6901 atcgccggaa tgaaatcgtc ggccacggtg cagcaaatgc agccattcga caattctatg
     6961 atattttctt ccgggcaggt ttcgacgccg cagccgcgga ggatttcgcc gtcgacgccg
     7021 acatcgccga attcgttgat cagcagggcc agcctgcggc cgcgcgcatt ttcgagaaga
     7081 tgccggatca acgtcgtctt gccggcgccg agaaagccgg tgacgatcgt ggtcggtatc
     7141 ttcgatacat caggcgaggc catcgtctgc tccgcattgt cgcggggcgc gaagaccggc
     7201 gctctcttcg cggagagcat aacgcggcgc catcgaaagc cccgcgagct ccgcatccag
     7261 gcgccccgcc cgatgcgaaa acggtcaagg gacggcaggt ctcctggctc gcggttcagc
     7321 gcttcgccat ccgccttccc aggtttgaga cccagtggcc ttggattgaa gctcgccgct
     7381 tacagttgcg ggggcagccg cggcttgggg ctgaaagccc gcaccgcatt ccctcttgct
     7441 ctccgtgatg gagaaccgtc cggcgcgcac gttgccaccg gcgcggcgcc ttgtcaaagt
     7501 aagctaatcg gccggcggct gctgtatgag gcgatcgcca tgacgcgttt gcgctcgggt
     7561 ctcgtgttcg cggccgtggc tttggccgcg ctcgctttgc caacggcgac ggccgccgct
     7621 gccgaacgcg cgctcctcct cgatatagac ggcccgattg ggccgccgat cgccgagtac
     7681 atcgtgcgcg agctcggcgc ggcgaagctg ggcgaggtgg ggctcgtcat tttgcggatg
     7741 aacacgccgg gcggcctcga tgcttcgatg cgccggatca tcagcgcgat cttggcctcg
     7801 cctgtcccgg tcgcgactta tgtcgcgccg aacggggccc gcgcggcgag cgccggcacg
     7861 tatatcgcct atgcgagcgc aatcgcggcg atggcgccca gcaccaatat cggcgcggct
     7921 acgccggttc aactcggcgg caatccgctc gcgccagagc aaaagccgca aaaagagcaa
     7981 aagcaggggg agggcggatc gcaggacgtg gaaacgcgca aaatcgtcaa cgacgccgtc
     8041 gcctatattc gcagccttgc cgcgctcaac ggccgcaatg ccgactgggc cgccgaggcg
     8101 gtgcgttccg cggcaagctt gccggcttcc gaagccctct cgcttcacgt cattgatacc
     8161 atcgccgaca atgtcccaga ccttttgcgc aagatcgacg gacgcaccgt gacggtcgac
     8221 ggcaagccgc agcgtctggc gaccgccggc cttgaggtgg tggccgcgcc gcccggctgg
     8281 cgcaccgagt tgctcgcgct tatcaccaat ccgaatgtcg ccttcatcct catgctcatc
     8341 ggcgtttacg gcctgatcct cgaattcttc aatcccggcg ccgtcgcgcc gggcctgatc
     8401 ggcgccatca gccttctcgt cgcgctttat gcgctggcct tgctgccgat caattacgcc
     8461 ggcgcagctt tggtgcttct cggcgtcgcc ctgatggtcg cggaggccca tatcggcgcc
     8521 ttcggcgcgt tgggcgtcgg cggcgtcgcc gccttcgttg tcggcgcgct gatgatgttc
     8581 ccggcgcggg cgccgggttt cgccctttcg ggcggagtta tagccggcgc cgcgatcggc
     8641 agcgcggcgc tgctgcttct gatccttgcg gccctgttgc gctcgcgcaa gcagccggtg
     8701 gtgaccggcg gcgaagcgct gatcggcgcc gagggcga
//
LOCUS       DATGJW010000496         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_823520, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000496 DATGJW010000000
VERSION     DATGJW010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_823520"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..431)
                     /locus_tag="VKU03_14915"
     CDS             complement(<1..431)
                     /locus_tag="VKU03_14915"
                     /EC_number="3.4.21.105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HLJ72609.1"
                     /translation="MRPLEEASPAGEPAPLKGREPIFDAPFVVIALAVLLLAIHAGMG
                     LISDAWQDVVARDLAFIPGRLTVALWPDALQHLLERANDNPEALRQAAMARHFHILEG
                     GAKAWTLLTYAFLHGGWAHVGLNVIWLVAFGPPVARRIGAPR"
     gene            844..1461
                     /locus_tag="VKU03_14920"
     CDS             844..1461
                     /locus_tag="VKU03_14920"
                     /inference="COORDINATES: protein motif:HMM:NF018960.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HLJ72610.1"
                     /translation="MKLAPSRELFAYWNSLRGARRAPERAEIDPIAISGLLADTFILE
                     IDPRAGYPFRVAGDRTGSLFQCELRGRPFLDIWQVSARNEIVEMLKTVTDEASPVVAG
                     VAAQTVGFHPLELELLVLPLRHRGATHSRVLGLCSPASSPTWLGLAPVGPMALHSMRV
                     LRQGDDRVGPAAARAGAAVPSQLESLARLMRRGQLHFFSSADPRP"
     gene            1569..2180
                     /locus_tag="VKU03_14925"
     CDS             1569..2180
                     /locus_tag="VKU03_14925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012333040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0035438 - cyclic-di-GMP binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PilZ domain-containing protein"
                     /protein_id="HLJ72611.1"
                     /translation="MTAEAAPLVDVKAIERRRHQRVKLVLLGRYMLEDRHEYPCQTID
                     ISPGGVAIEGAVKGQIGERVVAYFNQIGRVEGVIARHLPNGFAIQMRLPVLKREKLAE
                     QLTWLVNRQALGLPEDRRHERITPNFARTTLKTPDGQEYAARLIDISISGAAMNVDAA
                     PPIGSPVVVGSTPAQVVRHFSGGVAVEFSRLLPADTFDESARL"
     gene            complement(2190..2510)
                     /locus_tag="VKU03_14930"
     CDS             complement(2190..2510)
                     /locus_tag="VKU03_14930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72612.1"
                     /translation="MRRRIEVSTARFAGLVVNAISASRLAYWLIIAAMAFGLYEAHNA
                     GQTMRAMAEERVQRIIADEDRAFCEKFGFSAAGDAFASCRRELAIIRQNQADRDHADA
                     ASLL"
     gene            complement(2494..2817)
                     /locus_tag="VKU03_14935"
     CDS             complement(2494..2817)
                     /locus_tag="VKU03_14935"
                     /inference="COORDINATES: protein motif:HMM:NF020317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GYD domain-containing protein"
                     /protein_id="HLJ72613.1"
                     /translation="MGHYLVQLAYTAEAAKAMVKNPQNREATARAAMESLGGKVHSFF
                     FAFGEYDAIIIAELPNNIAAAAGALATTAGGAISKFHTTPLLTAGESVEAMKLAQKAV
                     YAPPH"
BASE COUNT          526 a          975 c          960 g          513 t
ORIGIN      
        1 cgcggcgcgc cgatgcgccg cgcgacgggc ggcccgaaag cgacgagcca gatgacgttg
       61 agcccgacat gcgcccagcc gccatggaga aaggcgtagg tcagcaaggt ccaggctttc
      121 gcgccgcctt cgaggatgtg aaaatggcgg gccatcgccg cttggcgcaa ggcttccgga
      181 ttgtcgttcg cccgctccag cagatgctgg agcgcgtcgg gccaaagagc gacagtcagg
      241 cggccgggaa tgaaagcgag atcgcgcgcc acgacgtcct gccaggcgtc ggaaatgagg
      301 cccatgccgg cgtgaatcgc caagagcagg acggcgagcg cgataacgac gaaaggcgcg
      361 tcgaaaatcg gctcgcggcc tttgagaggg gccggctcgc ccgcgggact ggcttcttcg
      421 agaggacgca tcggggactc ggggactggc gagttcccga taacacggcc gcggagcaga
      481 cgcgaacaaa ggagggcttg gagcaggccc tcctttgtcg cagtcagtca cgatttccgg
      541 cgcagcgcgt cggtcggtct ctcgagcggg ggagcgggcc cgcgagaagc cttggtcaaa
      601 aaacgggacc gcgccacgga gcaggcgaag gctcgtttca gcaacaagag tgtggcgcaa
      661 gaccgaagat cggacaatct tgacctaact ttatcattaa ttcggcgccc gagctttcga
      721 cccgccgtta accagccgcc gctggcatat cgcctgctcc cttttgctcc ggcagaaccg
      781 cgatcgccaa aatcgtgccc aaacaggacg gaggcgttcg acatcggtac cgcgggggac
      841 gagatgaaac tggcgccgag ccgagaatta ttcgcctatt ggaacagctt gcgcggcgcg
      901 cggcgcgcgc ccgagcgcgc cgagatcgat ccaatcgcga tcagcgggtt gcttgccgac
      961 acgttcattc tcgaaatcga tccgcgcgcc ggctatccct tccgggtcgc cggcgaccgg
     1021 acaggttcgc tcttccaatg cgaattgcgc ggccgcccct tcctcgacat ttggcaggtc
     1081 tcggcgcgca acgagattgt cgagatgctg aagaccgtca ccgacgaggc gagcccggtc
     1141 gttgccggcg tggcggcgca aaccgtcgga ttccacccgc ttgagctcga actgcttgtc
     1201 ctcccgctgc gtcatcgcgg ggcgacgcat tcgcgggttc tcggtctctg ctcgccggcg
     1261 tcttcgccga cttggctcgg cctcgcgccg gtcgggccga tggcgctcca ttcaatgcgc
     1321 gttctgcgcc aaggcgacga tcgtgtcggc cccgccgcgg cgcgcgccgg cgcggcggtc
     1381 cccagccagc tcgaaagctt ggcccggctg atgcggcgcg gccagcttca ctttttctcc
     1441 tcggccgatc cccggccttg aatcgtttgc cgcgcttcaa gtaatctgac cttaacccgt
     1501 ccgatcgtaa tcttaaccgc gacgcggggg catacgccgc gcgaagcgaa ttgaggagcg
     1561 agcgcgaagt gaccgccgag gctgcgcctc tcgtcgatgt caaggcgatc gagcggcgcc
     1621 ggcatcaacg ggtcaaactt gtcctcctcg gccgttacat gcttgaggat cggcacgaat
     1681 atccgtgcca aaccatcgac atatcgccgg gcggcgtggc catcgagggc gccgtcaagg
     1741 gccaaatcgg cgagcgcgtc gtcgcttatt tcaaccagat cggccgggtc gagggcgtca
     1801 tcgcgcgcca tttgccgaac ggcttcgcca tccagatgcg gctgccggtg ctcaagcgcg
     1861 aaaagctcgc cgagcagctc acctggctgg tcaaccgcca agcgctcggc ctgccggagg
     1921 accgccgcca cgagcgcatc acgccgaatt tcgccagaac cacgctgaag acgccggacg
     1981 ggcaggaata tgcggcgcgg ctgatcgaca tttcgatttc cggcgcggcg atgaatgtcg
     2041 acgcggcgcc gccgatcggc tcgccggtcg tggtcggctc gacgcccgct caggtcgtgc
     2101 gccatttcag cggcggcgtc gcggtcgaat tctcgcgcct gctgccggca gacacgttcg
     2161 acgaaagcgc ccgcctctaa ggcgcgccgc tacagcaagc tcgcggcgtc cgcatggtcg
     2221 cggtcggcct gattttgccg aatgatcgca agctcccgtc ggcatgaggc gaaggcgtcg
     2281 ccggcggcgc tgaagccgaa cttctcgcag aatgcgcggt cttcgtcggc gatgattcgc
     2341 tgcacgcgtt cttccgccat cgcccgcatc gtctggccgg cattgtgcgc ctcgtagagg
     2401 ccgaaggcca tggcggcgat gatgagccaa taggccaggc gcgacgccga gatcgcgttg
     2461 acgacgaggc ccgcgaaccg cgccgtactg acctcaatgc ggcggcgcat agacggcttt
     2521 ctgagcgagc ttcatcgcct ccacgctctc gcccgcggtc agcagcggcg tggtatggaa
     2581 tttcgaaatg gcgccgcccg ctgtcgtcgc cagcgctccg gcggccgccg cgatgttgtt
     2641 cggcaattcg gcgatgatga tcgcgtcgta ttcgccgaaa gcgaaaaaga aggaatgaac
     2701 cttcccgccg agggattcca tcgccgcgcg ggccgtcgcc tcgcgatttt gcggattttt
     2761 caccatcgcc ttggcggctt cggccgtata ggcgagttga acgagataat ggcccatttc
     2821 acgctccctc cctccgcctc gaccacatag tcgagcgccg agcccgatct aagaacggca
     2881 gcttcttcaa agtataacgt cgcctcgctc aacgcgtctg gaaatgattg cgcctatcgc
     2941 ccacggccta taagctttga acattccggc gttg
//
LOCUS       DATGJW010000497         2191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1027367, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000497 DATGJW010000000
VERSION     DATGJW010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2191
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1027367"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..817
                     /locus_tag="VKU03_14940"
     CDS             <1..817
                     /locus_tag="VKU03_14940"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012329910.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="substrate binding domain-containing protein"
                     /protein_id="HLJ72614.1"
                     /translation="RLLTRTTRSMNLTEAGRVFFERCVEIVEAVERAEASVAQDGGAN
                     PHGTLRVTAPLGLGRRVIAPMLARYRALQPQTDLRLRLSDHLLDLVQEGVDIAVRMAR
                     MEDSSFTLRKIAELRRVLCAAPAYIEARGRPQTPQDLANHDCLLLRYPGSQQFRWTLD
                     YRNEAVTVPISGPFDVDDGDVLTAWALDGLGIVLKPLFEVAEHIAAGRLVEVLPEAPP
                     QAVTLGLLYPTKRMLAPRAKTFIDLSVEQLRAHVTAKLALLKSAASAPAAETV"
     gene            1127..1849
                     /gene="tolQ"
                     /locus_tag="VKU03_14945"
     CDS             1127..1849
                     /gene="tolQ"
                     /locus_tag="VKU03_14945"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015078 - proton transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein TolQ"
                     /protein_id="HLJ72615.1"
                     /translation="MNPADAAQALAAPETTLLDMFWSAHIVVKFVMVGLLMASFWCWA
                     IIVNKTLLFTRFRRSMDRFEQSFWSGESLEELYGQLSNQENTGIASLFVSAMREWKRS
                     FQSAAASFMGLHARIEKVLDVSIAREVERLESNLLFLATVASASPFVGLFGTVWGIMT
                     SFRSIAASKNTSLAVVAPGIAEALLATAIGLFAAIPALIAYNRLQGDVAKAQARMESF
                     ADEFSSILSRQIDQHLGKDRAA"
     gene            1879..>2191
                     /locus_tag="VKU03_14950"
     CDS             1879..>2191
                     /locus_tag="VKU03_14950"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003613988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biopolymer transporter ExbD"
                     /protein_id="HLJ72616.1"
                     /translation="MGMSIAAAGRKGGRRRRGVPRYGAMADINMTPFIDVMLVLLIIF
                     MVAAPMAAVSVKVDLPPAVAKASPNPPKPVYISLQPNGRIFIMDKQTDLASLGDDLRE
                     SL"
BASE COUNT          335 a          759 c          717 g          380 t
ORIGIN      
        1 ccggctgctg acccgcacca cgcgcagcat gaatctcacc gaggccggcc gtgtgttttt
       61 cgagcgctgc gtcgagatcg tcgaggcggt ggagcgcgcc gaagccagcg tcgcccagga
      121 cggcggcgcc aacccgcacg gcacgctgcg cgtcaccgcg ccgctcggcc tggggcggcg
      181 ggtcatcgcg ccgatgctgg cgcgctatcg cgccctccag ccgcaaacgg acctgcggct
      241 gcgcctctcg gaccatctgc tcgatctggt ccaggagggc gtcgacatcg cggtgcgcat
      301 ggcgcggatg gaggattcga gcttcacgtt gcgcaagatc gccgagctgc gccgcgtgct
      361 ctgcgcggcg ccggcctata tcgaggcgcg cggccggccg cagacgccgc aggacctcgc
      421 aaaccacgat tgcttgctgc tgcgctatcc cggctcgcag caattccgct ggacgctcga
      481 ttaccgcaac gaggcggtca ccgtcccgat ttccggcccc ttcgacgtcg atgacggcga
      541 tgtgctgacc gcctgggcgc tggatgggct cggcatcgtg ctgaagccgc tgttcgaagt
      601 cgccgagcac atcgccgccg gccggctggt ggaggtgctg cccgaggcgc cgccgcaggc
      661 ggtcacgctc ggcctgctct atccgaccaa gcgcatgctg gcgccgcggg ccaagacctt
      721 catcgacctt tcggtcgagc agttgcgcgc gcatgtgacg gccaagctgg cgctgctcaa
      781 atccgccgct tcggcccccg ccgcggagac ggtctgacgc catgcgcaac ggcccttgtc
      841 gccgcggtcg ctgaccgcgg cgggcgcgac gcgccccgtt tcgcgtaaaa tcggaggcgg
      901 cgcggtcaac ggccgcgcct ccagcccgcc cggaaatttg ccccggttgc gctaacgccc
      961 tgttaaccct atcaggcgct taactcgatc gctagaaatc gccgccggag cgggcgcgcg
     1021 cggcagccca agtttcggcc catttgcggc gcagatcgcc cgaacacccc attttgagcc
     1081 cgccgcgcga cgcccgctgg ggccgccaga agcgaggtta tgcgacatga atcctgccga
     1141 cgccgcgcaa gcgctcgccg cgcccgagac gacgctgctg gacatgttct ggtcggccca
     1201 tatcgtggtc aaattcgtca tggtcgggtt gctgatggcg tcgttctggt gctgggcgat
     1261 catcgtcaac aagacgctgc tgttcacgcg cttccgccgc tcgatggatc gtttcgagca
     1321 atccttctgg tcgggcgagt cgctcgaaga gctctacggc cagctctcga accaggaaaa
     1381 taccggcatc gccagcctct tcgtctccgc gatgcgcgaa tggaagcgct ccttccagag
     1441 cgccgccgcc tcgttcatgg gcctgcacgc gaggatcgag aaagtgctcg acgtctcgat
     1501 tgcgcgggag gtcgagcggc tcgaatccaa tcttttgttt ctcgccaccg ttgcatcggc
     1561 cagcccgttt gtcggcctgt tcggcacagt ctggggcatt atgacctcgt tccgctcgat
     1621 cgccgcatcg aagaatacgt cgctggccgt ggtcgcgccg ggcatcgccg aagcgctgct
     1681 ggcgaccgcc atcggcctct tcgccgctat cccggcgctc atcgcctata atcggctgca
     1741 gggcgacgtc gccaaggcgc aggcgcggat ggagagcttc gccgacgaat tctcgtcgat
     1801 cctgtcgcgc cagatcgatc agcatctcgg caaggaccgc gccgcctaag cggcgcgccg
     1861 ggcaagggga attctgagat gggcatgtcg attgcggcgg cgggacgcaa aggcgggcgg
     1921 cggcggcgcg gcgtgccgcg ctacggcgcg atggccgaca tcaacatgac gccgttcatc
     1981 gacgtgatgc tggtcctgct gatcatcttc atggtggccg cgcccatggc ggcggtctcc
     2041 gtgaaggtgg atcttccgcc ggcggtggcg aaagcctcgc ccaaccctcc gaagccggtc
     2101 tatatctcgc tccagccgaa cggacgcatc ttcatcatgg acaagcagac cgacctcgcc
     2161 agccttggcg acgatctgcg ggaatccctg g
//
LOCUS       DATGJW010000498         8341 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_95592, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000498 DATGJW010000000
VERSION     DATGJW010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8341)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8341)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8341
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95592"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(168..1421)
                     /locus_tag="VKU03_14955"
     CDS             complement(168..1421)
                     /locus_tag="VKU03_14955"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009026923.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclopropane-fatty-acyl-phospholipid synthase
                     family protein"
                     /protein_id="HLJ72617.1"
                     /translation="MGVILRRMLPSLIRVGSLEIETADGFVGAYGDGTGPKIAVKVTD
                     KAAERALLLDPALALGELYMDGRVLVTRGDLYDLLALGARNMALEGGPRWLKWIEKAR
                     VLTRALRQRNDRFRARQHIAHHYDLNVRLYDLFLDLDRQYSCAYFERPTATLEEAQLA
                     KKRHIAAKLLVDEGHDVLDIGCGFGGMALYLAEMAGARAVGVTLSKEQHAVATKRAID
                     SGLADRVQFALKDYRDVEEKFDRIVSVGMFEHVGAPGYDEYFRTVRRLLKDDGVMILH
                     AIGRSAPPGATNPWIAKYIFPGGYIPAVSEVMASIERVGLYVTDTEILRLHYADTLKA
                     WRERFMARRDEAKAMYDERFCRMWEFYLAGSESSFRVDGHMVFQIQLAKSQDIVPRTR
                     DYVYDREAMLRRREGQKPALRLAGE"
     gene            1627..2343
                     /locus_tag="VKU03_14960"
     CDS             1627..2343
                     /locus_tag="VKU03_14960"
                     /inference="COORDINATES: protein motif:HMM:NF013718.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphatase PAP2 family protein"
                     /protein_id="HLJ72618.1"
                     /translation="MFRILALAATLALAVAQPAAAADFSASPSLLRYLRPGEIDLARI
                     LPPPPKPDSRADRRDLAISLAWQRSRTPAMIDLARADQDRSVFRFGVIFGGGFSKAQL
                     PKTAAFFDAVLSDERIVGAEAKTYWRRPRPFAASNLIRPCVDEPPTGSYPSNHATTAM
                     LYAEILARMLPERRRRIFARARQYARNRVICGVHYQTDIEAGERAGVAEARAMFKSAD
                     FRRDFAAARREIRAALPPRQ"
     gene            complement(2662..3099)
                     /locus_tag="VKU03_14965"
     CDS             complement(2662..3099)
                     /locus_tag="VKU03_14965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009491387.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HLJ72619.1"
                     /translation="MGVAFSLTKDEPPQNGGTGEEATIGEVAREFAVSLRTLRFYEDR
                     GLLQPRRQGASRLYNAADRRRLRIILKGKQLGFTLTEIRALIGAERDGEAEIEERLNA
                     EQIADQLGHLERQRGEIDAAIARLRAAQQRRSAHFSAQQPVAN"
     gene            3361..6966
                     /locus_tag="VKU03_14970"
     CDS             3361..6966
                     /locus_tag="VKU03_14970"
                     /inference="COORDINATES: protein motif:HMM:NF019843.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72620.1"
                     /translation="MSRAAAWNIRGIGRETREAAHEAARRAGVSLSEWLDEAIAQRAT
                     EQGVDPEDFDAEDRLDAIADRLNDLSSREDGRAHRHRGDEDMESDAPVRRFASREDMK
                     RAEDLLEAAIARFETRAAKAEERAARALDTVADWVEKRQASDSAGAEEALSAIRQVET
                     VARDLDKRISDLAQRLDASERKNREAARARPRIDLAEAVSQIARRRQALDGDQPAGAA
                     PRGQAEMAGAWRNFGVDLPAAPKEGEAGGMRGEIERLNQRLEELRRLQSEKPGAPDAD
                     LAALRAELSAMSRTLADLAPRNAVVAIEGAVRDLSERVASLRDNSLRDSLAKPVDEIV
                     AAIRETLQTHDPGKAVAGLEREIGAIGGRLDALARAKIEPDALERIRQQIEEVRAMLS
                     AAALRPMPVDRLEKQIGELADRVDRLSSSRSPHVDSAQVVASLANAHAEIERSTPAVA
                     LNAIERRLEDIAARMDQTLARGQSASSIGAKAVEELARRIDSVRETIETRHPLPIVSA
                     SLEATMRELAAKLQEAQQPRFDAEPMEAWMREVSAKLETARPVALDTKPLESWMRELT
                     AKLDGARPVSLDTGPLEQALRAIDDKISQAAAPQFQPKLVDETAQALARQIESKLKPA
                     VDVGAIEALIRGLEDKLQQTGGAKVPPEALGALRDQIGRIDARLDQTQGDLAALNSMG
                     RLVGQFSAKLDEMRESLRKAAADMAARGDAVAARDIEGLRAAQEASERRVQTTLGGLQ
                     GLLEKLVDRLTSIEEDVSHARAPAEPRPAPIAPPLSPDKPEPPPAKRSEPPPVTLADR
                     AAPRLGGGQQRGSDSPDFLIEPGAGAPAVAQPKAAINAHIAAARRAAQAASGEAGAAA
                     DKKSAIGAAPSATGRPIAQARAFLNARRRPILLGLAFVILGAIAVIELGSMYVVHSQK
                     SEIAAPPPKSASEAPAAPAKKEARAIDMTPTGAIAPVAPPPAAHKPPVPAPADLVALI
                     PPSAPQTLRDLAASGDPAAQDELASRLFEGRGMPRDIHAAAQWFERAANQDFAPAQYR
                     IGACYEKGNGVERDLALAKAWYEKAAQAGNIRAMHNLAVLLAEGPGVKPDYAEAAIWF
                     RKAAQHGVKDSQYNLAILYARGMGVPLDLNQSWLWFSLAAQQGDADAGKKRDEVAGKL
                     DANALAADNATLAAFRPRQADPDANEVKAPEGGWDAKAAAQTSEPPSKSRHATAL"
     gene            7022..7510
                     /locus_tag="VKU03_14975"
     CDS             7022..7510
                     /locus_tag="VKU03_14975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72621.1"
                     /translation="MSDWQAFFSAQVSASAALLGLLFVSISINLGKILSFRSLPNRAF
                     GALLILLVVLIVSSLLLAPGQARAAIGGEVLVIGLFAWAAITASDVKMWKNTDRAAAY
                     HRRLIPIAAINQLAALLYFVAGAVILTRGMIGVYWLVPAFLFSFAKAAMDAWVLLIEI
                     NR"
     gene            complement(7611..>8341)
                     /locus_tag="VKU03_14980"
     CDS             complement(7611..>8341)
                     /locus_tag="VKU03_14980"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013897047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72622.1"
                     /translation="RVVFITGGGSGIGAAMVDAFALQRANVAFVDILEAESHALVDRL
                     RHSGNPPLFIRCDLTDLSALEAAMQEVRERLGSIQVLVNNAANDWRHEADEVTEEFWD
                     RTLALNLKHQFFAAQAARRQMRERGGGSIVNFSSIAWMGGAPRLSVYATAKAGVVGLT
                     NSLAREFGADNIRVNAIAPGAVVTERQKKLWLSEADIKAFVSRQCIGRVLLAEDVARL
                     ALFLAADDSAMISKQCLVIDGGLR"
BASE COUNT         1389 a         2901 c         2748 g         1303 t
ORIGIN      
        1 gtcaaggctc gcgagccatc gcgccgcgcc aactgatttt ctcctgagcc tgctttggcg
       61 agcgacgcgc gaagcgcgct tggccttgac ggcgagcacg gctcaggcgc gccgcgcttc
      121 aagatgtctg catgccgtag ccgtcttcga caggggagga atgtccctta ctcccccgcg
      181 agccgcaagg ccggcttctg gccttcgcgc cggcgcagca tcgcctcgcg gtcataaaca
      241 taatcgcgcg tccgcggcac gatgtcctgg cttttggcga gctggatctg gaacaccatg
      301 tggccgtcga cgcggaacga ggattccgag ccggcgagat agaattccca catccggcag
      361 aagcgctcgt catacatcgc cttggcctcg tcgcgccgcg ccatgaagcg ctcgcgccag
      421 gctttcaggg tgtcagcgta atgcaggcgg aggatttcag tgtcggtgac gtaaaggccg
      481 acccgctcga tcgaagccat cacctccgat acggcgggaa tgtagccgcc cgggaagata
      541 tatttcgcga tccaaggatt ggtcgcgccg ggcggcgcgc tgcggccgat cgcatgcagg
      601 atcatcacgc cgtcgtcctt caggagccgg cgcacggtcc ggaaatattc gtcatagccc
      661 ggcgcgccga catgctcgaa catgccgaca gaaacgatgc ggtcgaactt ctcctcgacg
      721 tcgcgatagt ccttgagcgc gaattggacg cggtcggcga ggccggaatc gatggcgcgc
      781 ttggtcgcca ccgcatgctg ctccttcgac agcgtcacgc cgacggcgcg cgcgccggcc
      841 atttcggcga gatagagcgc catgccgccg aagccgcagc cgatatcgag cacgtcatgg
      901 ccttcgtcga ccagcagctt ggcggcgatg tgccgcttct tggcgagctg cgcttcttcc
      961 agcgttgccg tcggccgctc gaaataggcg caggaatatt gccggtcgag atcgagaaac
     1021 agatcgtaga ggcgcacatt gaggtcgtag tgatgcgcga tgtgctggcg cgccctgaag
     1081 cggtcgttgc gctggcgcag ggcccgcgtc agaacccgcg ccttctcgat ccatttgagc
     1141 cagcgcggcc cgccctccag cgccatattg cgcgcgccga gcgcgagcag atcgtagagg
     1201 tcgccgcgcg tcaccaggac gcggccgtcc atatagagtt cgccgagcgc caaagccggg
     1261 tcgagcagca gcgcccgctc ggcggctttg tccgtcacct tgacagcgat tttcggaccg
     1321 gtcccgtcgc cgtacgcccc gacgaatccg tccgcggttt cgatctcgag cgatccgacg
     1381 cggatcaagc tcggcagcat acgccgtaag ataacgccca tcgctccgcc cgccacgccg
     1441 tttcgccggc ggccaagagc gccggatcgc acagcttatc cttcgggcaa aactatgatg
     1501 gtcgcgcaac ggattcaagt tgcctcgcgc atggccgggt tgcggcgtgg cgcttgcgag
     1561 ccgccatggg atgaaacctt ggcggaatat cgctagaaga gagcctcgcc gctcaaagca
     1621 gacccgatgt ttcgtatcct tgccctcgcc gcaacactag cccttgctgt cgcgcagccc
     1681 gcggcggcgg cggatttcag cgccagcccc tccctgctgc gctacctgag gccgggtgag
     1741 atcgacctcg ccaggattct cccgccgccg cccaagccgg attcgcgcgc tgaccgccgg
     1801 gatctcgcga tcagcctcgc ctggcaaaga agccgtacgc cggccatgat cgatctggcg
     1861 cgcgccgacc aggatcgctc ggtgtttcgt tttggcgtga ttttcggcgg cggcttctcc
     1921 aaggcgcaat tgccgaagac cgccgccttt tttgacgccg tccttagcga cgagcgcatc
     1981 gtcggcgccg aggcgaagac atactggcgc cggccgaggc ctttcgccgc gtcgaatctg
     2041 atccggcctt gcgtcgacga accgccgacc ggctcctatc cgtcgaacca tgcgacgacc
     2101 gcaatgctct acgcggagat tctcgcgcgg atgctccccg agcgcaggcg gcggatcttc
     2161 gcgcgcgccc gtcaatacgc ccgcaaccgc gtcatctgcg gcgtgcatta tcagaccgac
     2221 atcgaggccg gcgagcgcgc cggcgtcgcc gaagcgcggg ccatgttcaa aagcgccgat
     2281 ttccgccgcg acttcgccgc ggcgcgccgc gaaatccgcg ccgcgctgcc gccgcgccaa
     2341 tagcaggcgc cgctcggcgg cgcgcgagga gccgcggccg atccaagcgg acgaaccgca
     2401 cggcggatgt gagcctgccc gtacctttgc cggcgctatg gcgcgttgcc ggcgatcggc
     2461 cggcgcggcg ccgaaggcta cattttgctt caacgatgcc gcagagaggg aatcgagacg
     2521 cgaaatcaac tccacgcgcc ggccacgcgc cggagcggtc ctggatttcc cagacaatcc
     2581 tgcccaaagc gggagcccag ccgacctcgt cgcgcgaacg accggccgca ctgggctccc
     2641 gccgccctcg cccgcggagt attaattggc gacgggctgc tgagcgctga aatgcgcgct
     2701 ccgccgctgc tgcgcagcgc gcagccgagc gatcgcggcg tcgatttcgc cgcgctggcg
     2761 ctcgagatgg ccgagctgat cggcgatctg ctcggcgttc aacctttcct cgatctccgc
     2821 ctcgccgtcg cgctcggcgc cgatcaacgc cctgatttcc gtcaaagtaa aaccgagctg
     2881 ctttcctttg agaatgattc gcaagcgccg gcgatcggcc gcgttgtaca agcggctggc
     2941 gccctgccgg cgcggctgca aaaggccgcg gtcctcgtaa aagcgcaatg tgcgcaagct
     3001 cacggcgaat tcgcgcgcca cctcgccgat tgtcgcctcc tcgcccgtcc cgccattctg
     3061 cggcggctcg tctttcgtca gtgaaaatgc gacgcccatg gctcccctcg ccctcctctt
     3121 tcgtttcaac ccccttccgt cgacggccgg tttgcgccgg ccctgtcggt tcaggaataa
     3181 tagttcaaat tttatctcta tgcaatctag agttgcagcg gcgggcattt tcaatcgccg
     3241 aacgggctgg cgcgtcgccg attttggccg ttcttaaccg tttgttcacc acgtcagccg
     3301 aaacatggct gagcgatcag cgggcgatgg cgtctcgcgt cgcttcggcg ttgggccagc
     3361 atgagcagag cggctgcctg gaatatcaga ggaatcggcc gtgagacgcg cgaggccgcc
     3421 cacgaggccg cccgccgcgc cggggtcagc ctcagcgagt ggctcgacga ggcgattgcc
     3481 cagcgggcga cggagcaggg cgtcgatcct gaagatttcg acgccgagga ccgtctcgac
     3541 gccattgccg atcggctcaa cgatctctcg agccgcgagg acgggcgggc gcatcgccat
     3601 cgcggcgacg aagacatgga gagcgacgcg ccggttcggc gctttgcgtc gcgcgaggat
     3661 atgaagcgcg ccgaggactt gctcgaagcg gcgatcgcgc ggttcgagac tcgcgccgcc
     3721 aaggccgagg agcgcgccgc gcgcgccctc gacacggtcg ccgactgggt cgaaaagcgg
     3781 caggccagcg attcggcggg cgccgaagag gcgctgagcg cgatacgcca agtcgagacg
     3841 gtcgcccgcg acctcgacaa gagaatcagc gacctcgcgc agcggctgga cgcttccgag
     3901 cgcaaaaacc gcgaagcggc gcgcgcgcgg ccgcgcatcg atctcgccga agcggtctcg
     3961 caaatcgccc gccgccgcca ggcgctcgac ggcgatcagc cggctggcgc tgcgccgcgc
     4021 gggcaagcgg agatggcggg cgcctggcgc aattttggcg tcgatcttcc ggccgcgccg
     4081 aaagagggcg aagccggcgg catgcgcggc gagattgagc ggctcaacca gcggctcgaa
     4141 gagttgcggc gcctgcaaag cgaaaaaccg ggcgcgccgg acgccgacct tgccgcgttg
     4201 cgcgccgaac tgtcggccat gtcgcgcacg ctcgccgatc tcgcgccgcg caatgcggtg
     4261 gtcgcgatcg aaggcgcggt gcgcgacctt tccgagcggg tcgcgtcgtt gcgcgacaat
     4321 tctttgcgcg attcgctcgc caagccggtc gacgaaatcg tcgcggcgat ccgcgagacg
     4381 ctgcagacgc atgatcccgg caaggcggtc gccggcttgg agcgcgaaat cggcgccatt
     4441 ggcggccggc tcgacgcgct ggcgcgggcg aaaatcgagc ccgacgcgct cgagcggatc
     4501 cgccagcaga tcgaagaagt gcgggcgatg ctgagcgcgg cggcgctgcg gcccatgccg
     4561 gtcgaccgcc tcgaaaagca gatcggcgag ctcgccgatc gcgtcgaccg cttaagctca
     4621 agccgctcgc cgcatgtcga ttcggcgcag gtcgtcgcct cgctcgccaa cgcccatgcc
     4681 gagatcgagc gctcgacgcc ggccgttgcg ctgaacgcga tcgagcggcg tctcgaagac
     4741 atcgccgccc gcatggatca gacgctcgcg cgcggccaat cggcgtcgtc gatcggcgcc
     4801 aaagcggtcg aggaattggc gcggcgcatc gacagcgtcc gcgaaacgat cgaaacccgc
     4861 catccgctgc cgatcgtcag cgcttcgctc gaagcgacga tgcgcgagct cgcggcgaaa
     4921 ttgcaggagg cgcaacagcc gcggttcgac gccgagccga tggaggcttg gatgcgcgag
     4981 gtgagcgcca agctcgagac cgcacggccg gtcgcgctcg acaccaagcc gctcgaaagc
     5041 tggatgcgcg agctgaccgc caagctcgac ggcgcccggc cggtcagcct cgataccggg
     5101 ccgctcgaac aggctttgcg ggcgatcgac gacaagattt cgcaagccgc cgcgccgcaa
     5161 ttccagccca agctcgtcga cgagacggcg caagccttgg cgcgccagat cgaatccaag
     5221 ctcaagccgg cggtcgatgt cggcgcgatc gaggcgctga tccgcggcct cgaagacaag
     5281 cttcagcaga ccggcggcgc caaagtcccg ccggaggcgc ttggcgcgct gcgcgaccag
     5341 atcggccgca tcgatgcgcg gctcgaccag acgcaaggcg atctggcggc cttgaattcg
     5401 atgggccggc tggttgggca attctcggcc aagctcgacg agatgcgcga gtctctccgc
     5461 aaggctgccg cggacatggc ggcgcgcggc gacgcggtcg cggcgcgcga catcgaaggc
     5521 ttgcgcgccg cgcaagaagc ttccgagcgg cgcgtccaga cgacgctcgg cggcttgcag
     5581 ggattgttgg aaaagctggt cgaccggttg accagcatcg aagaggacgt ctcgcacgcg
     5641 cgcgccccgg cggagccgcg ccccgctccc atcgctccgc ccttgagccc ggacaagccc
     5701 gagccgccgc cggccaagcg cagcgagccg ccgccggtca cgctcgccga tcgcgcagcg
     5761 ccgcggctcg gcggcggtca acagcgcggc agcgattcgc ccgactttct gatcgagccg
     5821 ggcgccggcg cgccggccgt ggcccagccc aaggccgcga tcaacgccca tatcgcagcg
     5881 gcgcggcgcg ccgcccaggc ggcttccggc gaggccggcg ccgcggcgga caaaaagtcc
     5941 gccatcggcg ccgcgccgtc cgcgaccgga cgccccatcg cccaggcgcg cgcctttctc
     6001 aacgcccgcc gccgcccgat cctgctcggc ctcgccttcg tcattctcgg cgcgatcgcg
     6061 gtgatcgagc tcggctcgat gtacgtcgtc catagccaga aatccgagat cgccgcgccg
     6121 ccgccgaaat cggcgagcga agcgccggcc gcgccggcca agaaagaggc ccgggcgatc
     6181 gacatgacgc cgaccggcgc gatcgcgccc gtcgcgccgc cgccggccgc gcacaagccg
     6241 ccggtccccg cgccggccga tctcgtcgcg ctgatcccgc catcggcgcc gcagaccttg
     6301 cgcgatctcg ccgcctccgg cgatccggcc gcgcaagacg agctcgcctc gcgcctcttc
     6361 gaggggcgcg gcatgccgcg cgacatccac gcggccgcgc aatggttcga gcgggccgcc
     6421 aatcaggatt tcgcgccggc ccaatatcgg atcggcgcct gctacgaaaa gggcaacggc
     6481 gtcgagcgcg atctggcgct cgccaaggcg tggtacgaga aagcggcgca agccggcaat
     6541 atccgcgcta tgcacaatct cgccgtcctg ctcgccgaag ggccgggcgt caagcccgac
     6601 tatgccgagg ccgcgatctg gttccgcaag gcggcgcagc atggcgtgaa ggacagccag
     6661 tacaatctcg ccatcctcta tgcgcgcggc atgggcgtgc cgctcgatct caatcagtcc
     6721 tggctgtggt tctcgctcgc cgcgcagcag ggcgacgccg acgccggcaa aaagcgcgac
     6781 gaagtcgccg gcaagctcga cgccaatgcg ctcgccgccg acaatgcgac gctggccgcc
     6841 ttcaggccgc gccaggccga tcccgacgcc aacgaggtca aagcgccgga aggcggctgg
     6901 gacgccaagg cggcggcgca gacgagcgag ccgccgagca agtcgcgtca cgcgacggcg
     6961 ctttgagcgc gtcgcgccga cgcggagagc tgatgcgccg ccaaagccgc aacgcgagcc
     7021 gatgagcgat tggcaggcct tcttttccgc tcaagtcagc gcctcggcgg cgcttctcgg
     7081 cctgcttttc gtcagcatct cgatcaatct cggcaagatt ctatccttcc gatccttgcc
     7141 gaatcgcgcc ttcggcgcgc tgctgatcct gctcgtcgtt ctgatcgttt catcgctcct
     7201 gctggcgccc ggtcaggcca gggcggcgat cggcggcgag gttctggtca tcggcctctt
     7261 cgcttgggcg gcgatcactg cgtccgacgt caagatgtgg aaaaacaccg atcgcgcggc
     7321 cgcttatcac cggcgcctca tcccgatcgc ggcgatcaat cagcttgcgg cgctgctcta
     7381 tttcgtcgcc ggcgcggtca tcctgacccg cgggatgatc ggcgtttatt ggctcgttcc
     7441 ggccttcctc ttctctttcg ccaaagcggc gatggacgcc tgggtgctgc tgatcgaaat
     7501 caaccggtag cctgcttatc attcccgcgg aaagcggcaa tcagggagcc tcgcgctttc
     7561 aggacgggcg ctgcctggat tcccgttttc gcaggaatgg acatgtcctc tcagcgcaag
     7621 ccgccgtcga tcaccaggca ctgtttgctg atcatcgcgc tgtcgtcggc ggccaggaac
     7681 aaagccagcc tcgccacgtc ctcggcgagg agcacccgac cgatgcattg gcgcgagacg
     7741 aaagccttga tgtcggcttc gctcagccag agcttctttt gccgttcggt gacgaccgcg
     7801 ccgggcgcga tggcgttgac gcggatattg tcggcgccaa attcgcgggc cagcgagttg
     7861 gtcaggccga caacgcccgc cttcgccgtc gcatagacgg aaagccgcgg cgcgccgccc
     7921 atccaggcga tcgaggagaa attgacgatc gagccgccgc cgcgctcgcg catctgccgc
     7981 cgcgccgcct gcgcggcgaa gaattgatgc ttcagattga gcgcgagcgt acgatcccag
     8041 aattcctcgg tcacctcgtc ggcttcgtgc cgccagtcat tggcggcgtt gttgacgagg
     8101 acctgaatcg atccgagtcg ctcgcgcacc tcctgcatcg ccgcctcgag cgccgacagg
     8161 tcggtcaggt cgcagcggat gaagagcggc ggattgccgg aatggcgcag ccggtcgacc
     8221 aaggcgtgcg actcggcttc cagaatgtcg acgaaggcga cattggcccg ctgcagggcg
     8281 aaagcgtcga ccatggccgc gccaatgccc gagccgccgc cggtgatgaa cacgacccgg
     8341 t
//
LOCUS       DATGJW010000499         4583 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_824063, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000499 DATGJW010000000
VERSION     DATGJW010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4583)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4583)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4583
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_824063"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(778..1929)
                     /locus_tag="VKU03_14985"
     CDS             complement(778..1929)
                     /locus_tag="VKU03_14985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006022503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3095 family protein"
                     /protein_id="HLJ72623.1"
                     /translation="MASGDQNFYANVPVFESFGDVMKPATYRPLPDGWMIGFSDVIGS
                     TKAIADGRYKAVNMVGAGVIAAVSNALKRKPFPFVFGGDGASFAIAAEDAPAAAEALK
                     AMAVFAREELQIELRVAMVPVAAVRKAGRDVRVGRFAASRFCAYAMFGGGGLAWVEME
                     AKSGAYHLEPAAPGSRPDLSDLSCRWSVAPAAHGAILSLIVAPRGEDERFAALVEEIV
                     QLAMAAQDGGRPITLSSLALPWPEQGIALEAATAPPGAARLLRRLLAAINFLGARIAV
                     PLKLKAGKFDAAEYVHDVVANSDFRKFDDGLRMTLDCSEEFADALERRLQQASDFAEY
                     GAFRQSNALLTCYAPSLTDRSHIHFVDGAGGGYAMAAKAMKERRLRAAA"
     gene            complement(2155..3825)
                     /gene="hutU"
                     /locus_tag="VKU03_14990"
     CDS             complement(2155..3825)
                     /gene="hutU"
                     /locus_tag="VKU03_14990"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018460324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HLJ72624.1"
                     /translation="MTANPRIDNARIIHAPRGPEISAKSWLTEAPLRMLMNNLDPEVA
                     ERPSELIVYGGIGRAARDWDAFDRIAAALTTLEADETLLIQSGKPVGIFRTHADAPRV
                     LIANSNIVPHWANWDYFHELDRKGLMMYGQMTAGSWIYIGSQGIVQGTYETFVEVGRR
                     HYGGDLSGRWILTAGLGGMGGAQPLAAVMAGASMLAVECQASRIEMRLRTGYLDRQAR
                     DLDEALAMIEDSCRAKQPLSVGLLGNAAEIFPELVRRGVRPDVVTDQTSAHDPLNGYL
                     PKGWTAAEWEARRERDPKSVEKAARASMAEHVRAMLDFYRKGVPTLDYGNNIRQMAQE
                     EGVQDAFDFPGFVPAYIRPLFCRGIGPFRWCALSGDPEDIYRTDAKVKELLPNNKHLH
                     NWLDMAKKRIKFQGLPARICWVGLGDRHRLGLAFNEMVANGELKAPIVIGRDHLDSGS
                     VASPNRETEAMKDGSDAVSDWPMLNALLNCASGATWVSLHHGGGVGMGFSQHAGMVIV
                     ADGTPEAARRIERVLWNDPASGVMRHADAGYDEAIACARANGLNLPSL"
     gene            complement(3903..>4583)
                     /locus_tag="VKU03_14995"
     CDS             complement(3903..>4583)
                     /locus_tag="VKU03_14995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013532302.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aromatic amino acid lyase"
                     /protein_id="HLJ72625.1"
                     /translation="QVMGACLDLLRQAATTLAVEANGVSDNPLVFSETGEVISGGNFH
                     AEPVAFAADMIAMALCEIGSLAERRIAMLVDPALSGLPAFLTPKPGLNSGFMIPQVTA
                     AALVSENKSRAYPASVDSIPTSANQEDHVSMAAHAARRLMPMIDNAFAVIGIELMVAA
                     QGCDFHAPLQSSARLESLRAAIRARIAHLDDDRHLRPDIEAATEVIRSGAAIAAVGAP
                     LPAICEAV"
BASE COUNT          776 a         1517 c         1509 g          781 t
ORIGIN      
        1 gatggattga ttgatcccct tctccccgct ccgcgcaggg ctgtccgggg aaaaaagctg
       61 cgcctcggcg gagcagttga cttactttcg acgtcggggc gtcggggcgc cacgccgccc
      121 gatcgccgtc aacccgccat agcgcctccc acgtgcgcgc aaaccacatc cgctcgcctg
      181 gcgctatggc gggtcctcca ctccgtttcg gcccttcggg tgactgcgat cgggcagcgt
      241 ggcttgttgg cgatcaagac gttttgctgc cgaaaccggc cgttcggccg tttcggtcag
      301 caaaacgtct tggtcgcaaa aggccatcga cgcgggcgcg cgtcaaggtt cgcgaagcga
      361 agcggagcga ccggcggagc cggcttgacc ttgacgcgcg tccgcgtcga tggcggcgcg
      421 gtctttggga ttttcttcgt ttgcgtctgt actcttcgca gaggctacga agaaaaccat
      481 ggccgtcgcc tgtcgctttc tgagtctcga catccaaaat ccaacgacgg aagtctcgaa
      541 agtccctcat tttgcgagct cgcgcctgcg acaactctat tcgtggcgat ttttccacgg
      601 acagccaagg cggcgagaag gtggcgcgaa gcgccggatg aggggcggcg caggactcga
      661 agagtactcg cggttcgaat cgctaatccc caagctcggc gcagaccctc acccggcggc
      721 ttcgccgcca ccctctcccc gcttcgcggg gcgaggggat tcgcgcagcg cggggcgtca
      781 cgccgcggcg cgcagccgcc gctccttcat cgccttcgcg gccatggcgt agccgccgcc
      841 ggcgccgtcg acgaaatgga tatggctgcg gtcggtcagc gagggcgcgt agcaggtcag
      901 cagcgcgttg ctttgccgga aggcgccgta ttcggcgaag tcgctcgcct gttgcaggcg
      961 tctctccaac gcgtcggcga actcctccga gcaatccaag gtcatgcgca agccgtcgtc
     1021 gaattttcgg aagtccgagt tcgcgaccac gtcgtggaca tattcggcgg cgtcgaattt
     1081 cccggccttc agcttcaagg gcacagcgat acgcgcgccc aaaaaattaa ttgcggcaag
     1141 aaggcgacgc agcaggcgcg ccgcgccggg cggcgcggtc gcggcttcaa gcgcaatgcc
     1201 ctgttcgggc caaggcaagg cgaggctgga gagcgtgatc ggccggccgc cgtcctgcgc
     1261 cgccatcgcc agttgcacga tctcttcgac gagggcggcg aagcgctcgt cctcgccgcg
     1321 cggggcgacg atcagcgaaa ggatcgcgcc atgcgcggcc ggcgcgacgc tccaccggca
     1381 ggaaaggtcg gagaggtcgg gccgcgagcc gggcgccgcc ggctccagat gataggcgcc
     1441 gctcttggcc tccatctcga cccaggcgag gccgccgccg ccgaacatcg cataggcgca
     1501 aaatcgcgaa gccgcgaagc ggccgacgcg gacatcgcgc ccggctttcc ggaccgccgc
     1561 gaccggaacc atcgcgacac gcaactcgat ctgcaactcc tcgcgggcga agacggccat
     1621 cgctttgagc gcctcagccg ccgccggcgc atcctccgct gcgatggcga agctcgcccc
     1681 gtcgccgccg aacacgaacg gaaagggctt gcgcttcagc gcattggaga ccgcggcaat
     1741 cacgccggcg ccgaccatgt tcacggcctt gtagcgtccg tcggcgatcg ccttggtcga
     1801 gccgatgacg tccgaaaacc cgatcatcca accgtcgggc agcggccgat aggtggccgg
     1861 cttcatcacg tccccgaaac tctcgaagac gggaacattc gcgtagaaat tctgatcgcc
     1921 gctcgccatg gcgccctcca ccggttaggc ggctcggccc attctatcgt gattacgatc
     1981 gcggcgcagc ggagtttcac gctttcctcc cccgtgaagc gaagcggaac ggggggacca
     2041 tgcgaagcat ggtggagggg gcttcggcgg ttgagaatta tcctgagaga ccgaagcccc
     2101 ctccaccgcg ctgacgcgcg gtccccctcc cccgtctttc gacgggggag gatattacag
     2161 actcggcaga ttgagcccat tcgcccgggc gcaggcgatg gcttcgtcat agccggcgtc
     2221 ggcgtggcgc atgacgccgc tggccgggtc gttccacaag acgcgctcga tgcgccgcgc
     2281 cgcctccggc gtgccgtcgg cgacgatcac catgccggcg tgctgcgaga agcccatgcc
     2341 gacgccgccg ccgtgatgca gcgacaccca ggtcgcgccg gaggcgcagt tgaggagcgc
     2401 gttcagcatc ggccagtcgg aaaccgcgtc ggagccgtcc ttcatcgcct cggtctcgcg
     2461 attgggcgag gcgaccgagc cggaatcgag atgatcgcgg ccgatgacga tcggcgcttt
     2521 caactcgcca ttggcgacca tctcgttgaa ggcgaggccg aggcgatggc ggtcgccgag
     2581 gccgacccag cagatgcgcg ccggcaggcc ctggaacttg atgcgcttct tcgccatgtc
     2641 cagccaattg tggagatgct tgttgttggg cagcagctct ttcaccttgg cgtcggtacg
     2701 atagatatcc tccggatcgc cggaaagcgc gcaccagcgg aacggtccga tcccgcggca
     2761 gaagagcggc ctgatatagg cggggacgaa gccgggaaag tcgaatgcgt cctgtacccc
     2821 ttcctcctgc gccatctggc gaatgttgtt gccgtagtcg agcgtcggca cgcctttgcg
     2881 gtaaaaatcg agcatcgcgc gcacatgctc ggccatcgac gcgcgcgcgg ccttctcgac
     2941 gcttttggga tcgcgctcgc gccgcgcctc ccattccgcc gcggtccagc ctttcggcag
     3001 atagccgttg agcgggtcat gcgcagaggt ctggtcggtg accacgtccg gccgcacgcc
     3061 gcggcggacg agttcgggga aaatttccgc tgcgttgccg agcaacccga ccgaaagcgg
     3121 ctgcttggcg cggcaagagt cttcgatcat cgccaaagct tcgtcgagat cgcgcgcctg
     3181 ccggtcgaga tagccggtgc gcagccgcat ctcgatgcgg ctcgcctggc attcgacggc
     3241 gagcatcgaa gcgccggcca tgacggcggc gagcggctgc gcgccgccca tcccgcccaa
     3301 gccggcggtc agaatccacc tgccggaaag gtcgccgcca taatgccggc ggccgacctc
     3361 gacgaaggtc tcgtacgtgc cctgcacgat gccctggctg ccgatataga tccacgagcc
     3421 tgccgtcatc tggccgtaca tcatcaggcc tttgcggtcg agctcgtgga aatagtccca
     3481 attcgcccaa tgcggaacga tgttggaatt ggcgatcagc acgcgcggcg cgtcggcatg
     3541 ggtgcggaag attccgaccg gcttgccgga ttggatcagc agcgtctcgt ccgcttcgag
     3601 cgttgtcagc gccgcggcga tgcggtcgaa agcgtcccaa tcgcgcgcag cgcggccgat
     3661 gccgccatag acgatgagct cgctcggccg ttcggcgact tcgggatcga gattgttcat
     3721 cagcatgcgc agcggcgcct cggtcagcca gcttttagcg gatatttccg ggccgcgcgg
     3781 cgcgtgaatg atgcgggcgt tgtcgatgcg gggattggcg gtcatggcgg tcactaaggc
     3841 ctcgctgatc aaatttcgtc attgcttcgt cgcttcgctc gcaatgacgg tacatctttt
     3901 catcacacgg cttcgcagat cgcaggcaga ggcgcgccga ccgccgcgat cgccgcgccg
     3961 ctgcggatga cctccgtcgc cgcttcgatg tcgggccgca aatgccggtc gtcgtcgaga
     4021 tgggcgatcc gcgcccttat cgccgcgcgc aaggattcga gccgcgcgct cgattgtaaa
     4081 ggcgcatgga agtcgcagcc ttgcgccgcg accatcagtt cgatgccgat caccgcgaaa
     4141 gcgttgtcga tcatcggcat gagccgccgc gccgcgtgcg cggccatcga aacgtgatct
     4201 tcctgattgg cggaagtcgg aatcgaatcg acgcttgccg gataggcccg gctcttgttc
     4261 tccgagacga gcgcggctgc ggtgacctgc gggatcatga agccggaatt gaggcccggc
     4321 ttcggcgtca ggaaagccgg caggccggaa agagccgggt cgaccagcat ggcgatgcgg
     4381 cgctcggcga gcgagccgat ctcgcacagc gccatcgcga tcatgtcggc tgcgaacgcg
     4441 acgggctccg cgtggaaatt gccgcccgag atcacttcgc cggtctcgga gaagaccagc
     4501 ggattgtccg aaacgccatt cgcctcgacg gcgagcgtcg tcgcggcctg gcgcaagagg
     4561 tcgaggcaag cccccatcac ctg
//
LOCUS       DATGJW010000500        12184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_717121, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000500 DATGJW010000000
VERSION     DATGJW010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12184
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_717121"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..292)
                     /locus_tag="VKU03_15000"
     CDS             complement(<1..292)
                     /locus_tag="VKU03_15000"
                     /EC_number="2.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003577480.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acetyltransferase"
                     /protein_id="HLJ72626.1"
                     /translation="MSPADPIVIVGAARTPMGGFQGDLKDVAAPALGAAAIEAAVGRA
                     GVKPEAVEEVLFGCVLPAGQGQAPARQAAVAAGLPMAAGCTTVNKMCGSGMKA"
     gene            364..1164
                     /gene="moeB"
                     /locus_tag="VKU03_15005"
     CDS             364..1164
                     /gene="moeB"
                     /locus_tag="VKU03_15005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HLJ72627.1"
                     /translation="MTLSSEEIERYARHIVLRGVGGPGQNKLKAARVLAIGAGGLGSP
                     LLQYLAAAGIGKIGVVDDDVVSLSNLQRQVLHGTDDVGRPKVDSAAAALRRINPHVEV
                     ETIPERLDETNARALIAPYDVVADGSDNFATRYIVSDACFYERKPLVTAAIGEYDGSL
                     TVLKPYARNKDGALNPTYRCLFPEPPPPGMVPACAESGVLGALAGLVGSLMALEVVRE
                     IVGGFDEGDQGLVGKLLLIDTRAMRFETLTYAWDENNPLSGKASVLSR"
     gene            complement(1258..1737)
                     /gene="msrB"
                     /locus_tag="VKU03_15010"
     CDS             complement(1258..1737)
                     /gene="msrB"
                     /locus_tag="VKU03_15010"
                     /EC_number="1.8.4.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003543198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033743 - peptide-methionine (R)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (R)-S-oxide reductase MsrB"
                     /protein_id="HLJ72628.1"
                     /translation="MLTRRVLLAGAATGLAAGVFSRPGRAAEMFEVTHTDAEWRKLLT
                     PEQFAILRESATERPFTSPLLNEHRRGLFACAGCDRDLFSSETKFDSGTGWPSFWAPL
                     DKAVGTTEDTSFGMTRTAVHCSRCGGHLGHVFDDGPKPTGLRYCMNGLALSFKPATA"
     gene            1932..2007
                     /locus_tag="VKU03_15015"
     tRNA            1932..2007
                     /locus_tag="VKU03_15015"
                     /product="tRNA-Glu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1966..1968,aa:Glu,seq:ctc)
     gene            2083..2892
                     /locus_tag="VKU03_15020"
     CDS             2083..2892
                     /locus_tag="VKU03_15020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007614332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent protein deacetylase"
                     /protein_id="HLJ72629.1"
                     /translation="MTPFSTLEEFIERHRKLFILTGAGCSTRSGIPDYRDADGAWKRA
                     QPIRYQDFMADAAARRRYWARSLIGWRRFREARPNDAHRALARLESKDRIELLLTQNV
                     DRLHQAAGSVRVVDLHGRLDLVRCMGCGRRIARDEVQDELGRLNAGWLGLAAADAPDG
                     DADLDGADFSSFEPPSCRRCGGVLKPDVVFFGETVPRGRVDAAMRHLAQADAMLIVGS
                     SLMVYSGYRFAQTAVSAGKPAAAINLGRTRADGLLALKVEQACEGALAFLL"
     gene            complement(3053..3403)
                     /locus_tag="VKU03_15025"
     CDS             complement(3053..3403)
                     /locus_tag="VKU03_15025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614712.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72630.1"
                     /translation="MSLAYDLFAQAMHEEKQIFCMYDGYPRELCPIVLGHTNGQEKAL
                     TFQVGGRSSKGALPNGGEWRCLFLAKVRDARLRSGPLRAGDRHSRPQDCVEEVDLDIN
                     PDSPYHPKRRIRRS"
     gene            complement(3538..3849)
                     /locus_tag="VKU03_15030"
     CDS             complement(3538..3849)
                     /locus_tag="VKU03_15030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011665431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0030261 - chromosome condensation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HU family DNA-binding protein"
                     /protein_id="HLJ72631.1"
                     /translation="MATKLMSKSELIQKIADGHPSGLSRKDVKTVIESLAEIGHKELK
                     KAGAFLVPGMAKFVVIKKPATKARKGINPFTKEPTVFKAKPARKIIRARPVKAAKDAV
                     A"
     gene            complement(4143..6083)
                     /locus_tag="VKU03_15035"
     CDS             complement(4143..6083)
                     /locus_tag="VKU03_15035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894232.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 127 protein"
                     /protein_id="HLJ72632.1"
                     /translation="MPAPTPSKPRARFRPPATPQVEVGGFWGGRIDAVADRTADILYE
                     RCVAAGMLDQIDPDRPSPGVKIPFHIRDGETSTVTTQMFWDSDLGKTIETAAYSLYRK
                     ANPALEAKIDAVIDMYAKLQQPDGYLSSWYQRIQPGRRWTNLRDCHELYCAGHLIEGA
                     VAYDQATGKRKLLDVMRRYADHIASVFGPEPEKKKGYCGHEEIELALVKLARATGERK
                     YLDLAKYFIDQRGQQPHYFDIEARARGADPKDFHFNSYEYNQSHRPVREQDKVVGHAV
                     RAMYLYSGMADIATEYGDDELKAALDRLWADLTGKRLYITGGLGPSADNEGFTSDYDL
                     PNETAYAETCAAVGLVFWASRMLGMGPDSRYADVMERALYNGAISGLSLDGSRFFYEN
                     PLESRGAHHRWRWHRCPCCPPNIARLTASIGSYMYGVADDAIAVHLYGDNVARLAVAG
                     REIRLTQRSRYPWDGAVALTIDTEAPVFFALHLRIPGWSRKAALSLNGEPIDLAAAAE
                     NGYARLAREWRRGDRVQLDLDMAAVRISAHPEVRQDAGRVALMRGPLVYCLEGADNPV
                     GLNRLRAPAAARFECRFEPDLLDGVATLSAPAAADATDDWGDILYRTEPARTEPATIK
                     AVPYFAWDNRAPGEMLVWLRGD"
     gene            complement(6275..6874)
                     /gene="bcsS"
                     /locus_tag="VKU03_15040"
     CDS             complement(6275..6874)
                     /gene="bcsS"
                     /locus_tag="VKU03_15040"
                     /inference="COORDINATES: protein motif:HMM:NF028346.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cellulose biosynthesis protein BcsS"
                     /protein_id="HLJ72633.1"
                     /translation="MLRLVFCACAFLAFIAGARADEANDLLYFCGDLISGRNYLGVGW
                     RHAFSGLDASGLVFSMEGGAPAWRRFFAAGEAGWRFAQPGFALTFMAGAEVDPRPHPL
                     ASADLWIEPTPHWMAQANVAVATDWTSWRVATGWRANERWPWLGPEAAASAGFPRIGL
                     HATGLKLIASMEARLSIGASWRQGRRPGPYAELSAWRRF"
     gene            complement(7005..8264)
                     /gene="mtnK"
                     /locus_tag="VKU03_15045"
     CDS             complement(7005..8264)
                     /gene="mtnK"
                     /locus_tag="VKU03_15045"
                     /EC_number="2.7.1.100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271851.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046522 - S-methyl-5-thioribose kinase
                     activity [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5-thioribose kinase"
                     /protein_id="HLJ72634.1"
                     /translation="MASAYAAVDGAKLSSILAGIPDVRTRLGGEPDAWRVREVGDGNL
                     NLVFIVEGPEGGVVVKQALPYVRLVGESWPLPLERSWFEYNALVEQSRYAPRLTPKLF
                     HFDRDQAMIVMEYLSPHIIMRKGLIRGVEYPRFAADIAEFLAETLFNTSVLAGSAVEH
                     KRRVALFAANTELCRITEDLVFTDPYREAPLNRWNRPHLDADKRAFEADSALKFAAQA
                     RKYQFITATQALIHGDLHTGSIMLTTEDTRVIDPEFAFVGPIGFDVGAVIGNLFLAFC
                     AQEGHEASPGARDAYRAWILDQVTAVWTGFHDRFLTLWRKAKGGEAYVASLVVSPQDA
                     AAMDAERARFMRRLFEDALAFGGLKMIRRILGLAHVEDLERIADPAVRAVCERKALLI
                     GRELVLGATAFTDIADVTAAARAAIRA"
     gene            complement(8292..9326)
                     /locus_tag="VKU03_15050"
     CDS             complement(8292..9326)
                     /locus_tag="VKU03_15050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72635.1"
                     /translation="MDAPKANVVAAERPFRVGDFLRNQLRNIAPFITLLALVTFFSVA
                     SPSFATVDNLVNILQQVSVTGIIAVGLTFVILTAEIDLSVGAIANATAIVLTFFTLQP
                     DYVNIANIPLPGAVAIVFALLACFALGLVTAFGVTKIGIPSFIMTLAMMQIGAGISAV
                     LVRGQIAYTVPSLIQTLGSKSIAHVPWIIIVAALFLLVAHLVLTYTRFGRYVYMVGGN
                     REAAEYSGVNTKLIVGAVMVISAVCSGVAGMIGVAYFGSAQQNEFDTFLLDSIAAVVV
                     GGTSLFGGRGGIPNTIVGLFVLGVLNNGLDYVKIDSFLKILIRGLILLIALVINIYAE
                     RMRTASRSAG"
     gene            complement(9342..10838)
                     /locus_tag="VKU03_15055"
     CDS             complement(9342..10838)
                     /locus_tag="VKU03_15055"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008968440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ72636.1"
                     /translation="MQPALLELSGITKSFGGVQALRAVDFALNAGEIHGLVGENGAGK
                     STLMKIIAGVHADYEGSMRVDGREVHFRSSRDALAAGIGMVHQELSVAPELTVAENVF
                     LGMQPVNRLGIVAWRKMARQAKAQLQNLGLDIDPRARLGDFPIGVQQLVELSRVLFSG
                     ARIIILDEPTSALSPPEIERLFDVLVKLKQSGRSLIFISHFLDDILKVSDEITIFRNG
                     RKVATTPVTAEVDKGWIIERMIGAGREELEESYLGEIRLASKPGAPIVLEAKGLTLGA
                     AYRDVTFAARGGEVLGVYGFMGCGQLELARTLFGKLKPDRGSLAIGGAPARLRNTSAA
                     QKAGVAYVAESRRAMLFGEEPVYKNVSIAVLARLSRFLLKPDQERALAAEQVKALDIR
                     PPNVELRLGGLSGGNQQKVALAKWLTHPPRILLLAEPTRGMDVGAKEDVVKIVRALRD
                     QGIAIIVFSTEPETVLSLADRVLVMRKGEIAHEFADEAISKDRLLAAA"
     gene            complement(11046..12062)
                     /locus_tag="VKU03_15060"
     CDS             complement(11046..12062)
                     /locus_tag="VKU03_15060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ72637.1"
                     /translation="MTQTRRDLIRSAAVGGGAASLFGGFGFNPLITTAMAKEAGKATK
                     PLKAAFSNAGLQATWCAQGKQAAEYWGSLFNVEVTWFDGQLDAVKQRSAIDNMASQKW
                     DFVAIQAFGIGTLTAPVNKMIDAGIPVIDMDTLIAPLDQIKVLSFLAPDNEFMGASVT
                     QALVNAINGEGTIVMTQGALGHTGAQGRARGFKSVVSKFPKIQVLDEQPADWDVTKVA
                     RIWETLLTKYPKIDAAFFHNDDMALAAYKVMQAKGRTETKIGGVDAMPPAIKAVQDGQ
                     MYATVRNPSCRIHGGAIIAGVSAVINGAKTGTDIPKNIVTDGPVVTKANADGMLWMED
                     QFLI"
BASE COUNT         2111 a         3890 c         4127 g         2056 t
ORIGIN      
        1 ccgccttcat gcccgagccg cacatcttgt tgaccgtggt gcagccggcg gccatcggca
       61 ggccggcggc gacggcggct tggcgggccg gggcctggcc ctggccggcc ggcaggacgc
      121 agccgaacag cacctcctcg accgcttccg gcttgacccc ggcgcgcccg accgccgcct
      181 cgatcgccgc cgcgccgagc gcgggggcgg cgacatcctt cagatcgccc tggaagcctc
      241 ccatcggcgt gcgcgccgcg ccgacaatga cgatcgggtc tgcaggcgac atgggctttt
      301 cctttcaggg cgccgggcgg ccggattgca tgcccggctg atggcgatta aatggccgcc
      361 aggatgacgc tttcaagcga agagatcgag cgctacgccc gccatatcgt gctgcgcggc
      421 gtcggcggcc ccggccaaaa caagctgaaa gcggcgcgcg tcctggcgat cggggccggc
      481 gggctcggct cgccgctgct gcaatatctc gccgccgccg gcatcggcaa gatcggcgtc
      541 gtcgacgacg acgtcgtctc gctctccaat ttgcagcggc aggtcctgca cgggacggac
      601 gatgtcggcc ggccgaaagt cgacagcgcg gcggcggcgc tgcgccgcat caatccgcat
      661 gtcgaggtgg agacgatccc cgagcggctt gacgagacca atgcgcgcgc gctgatcgcg
      721 ccttacgacg tggtcgccga cggatcggac aatttcgcca cccgctacat cgtctcggac
      781 gcctgcttct atgaacgcaa gcccttggtg acggcggcga tcggcgagta cgacggctcg
      841 ctgacggtgc tgaagcccta cgcccgcaac aaggacggcg cgctcaatcc aacctatcgc
      901 tgcctgtttc ccgagccgcc gccgccgggc atggtcccgg cctgcgccga gagcggcgtt
      961 ctcggcgcgc tggccggcct ggtcggctcg ctgatggcgc tggaagtcgt ccgcgagatc
     1021 gtcggcggct tcgacgaggg cgatcaaggc ctcgtcggca agctgctgct gatcgacacg
     1081 cgcgcgatgc gcttcgagac gctgacctac gcctgggacg agaacaatcc gctgagcggg
     1141 aaggcgtcgg ttctgtcgcg ctgagcgccg tcgttgcgag gagcgcagcg acgaagcaat
     1201 cgcctcatag ggcggcgacc tgtgagggga tcgcttcgct cgcaaggacg gggcgcttca
     1261 cgccgtcgcc ggcttgaacg acagagcgag gccgttcatg caatagcgca ggcccgtcgg
     1321 cttggggccg tcgtcgaaga catggccgag atggccgccg cagcggctgc aatggacggc
     1381 ggtgcgggtc atgccgaagg acgtgtcctc ggtcgtgccg accgccttgt ctaacggcgc
     1441 ccagaagctc ggccagccgg tgccgctgtc gaacttggtc tccgacgaga agaggtcgcg
     1501 atcgcagccg gcgcaggcga agaggccgcg gcgatgctcg ttgaggagcg ggctggtgaa
     1561 gggccgctcg gtcgccgatt cgcgcaagat cgcaaattgc tccggcgtca ggagcttgcg
     1621 ccattcggcg tcggtatggg tgacctcgaa catttccgcc gcccggccag gccgcgaaaa
     1681 gacgccggcc gccaatcccg tcgcggcgcc ggccaaaaga acgcgtctgg tgagcatgat
     1741 gaaccctccc atttcaggaa cacgcttgcc ggcagatacg gcgggcgcgg cgattttgtt
     1801 acacgccaag ggcttatcac gggcttgtga gcgatttgag gccgcctctc cgcctgcggc
     1861 gcggggccga agtctttcgc ttgcgttcgc tctgcgccgc cgatatagac ccggcttcgc
     1921 ttgaaagcgc cgctcccttc gtctagcggt ccaggacgtc gccctctcac ggcgaaaaca
     1981 ggggttcgag tcccctaggg agcgccaatc atgcggcgac gccgcgccct cttggcgcgg
     2041 tttcaacggc ctcgccgcaa cgcgtacgcc ccagcggtac tcgtgacgcc atttagcacc
     2101 cttgaggaat ttatcgagcg ccatcggaag ctcttcatcc tgaccggcgc cgggtgcagc
     2161 acgagatcgg gcattcccga ttaccgcgac gcggacggcg cctggaagcg ggcgcagccg
     2221 atcagatacc aagacttcat ggccgacgcc gcggcgcggc gtcgctattg ggcgcggagc
     2281 ctgatcggct ggcggcgctt tcgcgaggcg aggccgaatg acgcccatcg cgccttggcg
     2341 cgcctcgagt ccaaagaccg gatcgagctt cttctgactc agaacgtcga ccggctgcat
     2401 caagccgccg gcagcgtccg cgtcgtcgac ctgcatgggc ggctcgacct tgtccgctgc
     2461 atgggctgcg ggcgcagaat cgcgcgcgac gaagtccagg acgagcttgg ccggctgaac
     2521 gccggctggc tgggactggc cgccgccgat gcgccggacg gcgacgccga tctcgacggc
     2581 gccgacttct cctcgttcga gccgccgtca tgccgccgct gcggcggcgt cctcaagccg
     2641 gacgtcgtct tcttcggcga gacggtcccg cgcggccgcg tcgacgcggc catgcgacat
     2701 ttggcgcaag ccgacgcgat gctgattgtc ggctcctcgc tgatggtcta ttccggctac
     2761 cgcttcgctc aaacagccgt ttcggccgga aagcccgcag ccgcgatcaa tctcggccgg
     2821 acgcgcgccg acgggctgct cgcgctgaaa gtggagcagg cgtgcgaggg cgcgctggct
     2881 tttcttttgt aagggatagg gtcggagggg aacgggtgct ttcaggacaa ggaccgcacg
     2941 cgctcgaagc gctcagaaga tacatgacca tcatctgcag cgcgtccgcc tctgcgatga
     3001 ttgtcgcgat cgcctggaat ttgctgacgc gctgagccgg gcgagccgcg gctcaagagc
     3061 gccggatgcg acgcttcgga tggtacggac tgtcgggatt gatgtcgagg tcgacctcct
     3121 cgacgcagtc ttgcggccgg ctatggcggt cgccggcccg caagggcccg ctccgcaagc
     3181 gcgcgtcgcg gactttcgcc aggaacaggc agcgccattc gccgccgttc ggcaaagcgc
     3241 ctttgctgct gcgacccccg acctggaagg tcagcgcttt ttcctgcccg ttggtatggc
     3301 cgagaacgat cgggcatagc tcgcggggat agccgtcata catgcagaag atctgcttct
     3361 cttcatgcat ggcttgggcg aacagatcgt aggcgaggct catggcggag cgttgccgag
     3421 cggccaaccg cgcatcgccg gcgattctcg gaagagaaag actggcgggc gcctgcggcg
     3481 ccgcgccagt cttaaaagcc gataacgcgc ccttattggc cgcgggcttt ccgcggctta
     3541 agccacggcg tccttggccg ccttgaccgg gcgggcccgg atgattttcc gcgccggctt
     3601 cgccttgaac acggtcggct ccttggtgaa gggattgatc cccttgcgcg ccttggtcgc
     3661 cggcttcttg atcaccacga acttggccat gccgggcaca agaaaagcgc cggccttttt
     3721 tagctccttg tgaccgatct cggcgagcga ctcgataacg gttttgacgt ccttgcggga
     3781 cagtccgctt ggatgcccat cagcgatttt ctggatgagc tccgatttcg acatcagctt
     3841 ggtagccatc ttttctcctc tgggtgcgaa tcagcgagac acacgttatg cagcggcgcg
     3901 ccctatgtat aggcgggcgc gcaacacttt tccccagcgc atagcgcgaa aatagcctaa
     3961 aaatgggctg ttccgggcgt tcggccgccc gaggcgggcg cgccgagccc gcctttggcg
     4021 gcgcaaacaa ggggcggcgt gggacgagcc gccgtccggg ccgcggcgcc gaagcggccg
     4081 aaatcccgct aaaaacggcc tgttcgggct cgatggccgc cgtggcgaac cgggcggagc
     4141 ggctagtcgc cgcgaagcca gacgagcatt tctcccggcg cgcgattgtc ccaggcaaag
     4201 tacggaaccg ccttgatcgt cgccggctcg gtcctcgccg gctcggtacg atagagaatg
     4261 tcgccccaat cgtcggtcgc atcggcggcc gcgggcgccg agagcgtcgc cacgccgtcg
     4321 agaagatcgg gctcgaagcg gcattcgaac cgcgccgcag ccggcgcgcg caggcggttg
     4381 aggccgaccg ggttatcggc tccttcgagg caatagacca gcgggccgcg catcaaggcg
     4441 acgcggccgg cgtcctggcg cacctccggg tgcgcgctga tccgcacagc cgccatgtcg
     4501 aggtcgagtt gaacgcggtc gccgcgccgc cattcgcgcg cgaggcgggc gtagccgttc
     4561 tcggcggctg cggcgagatc gatcggctcg ccgttgagcg acaaggccgc cttgcggctc
     4621 caaccgggaa tgcgcagatg cagcgcaaag aaaaccggcg cctccgtatc gatcgtcagc
     4681 gcgaccgcgc cgtcccatgg atagcggctc cgttgagtca aacgaatctc cctaccggcg
     4741 acggcgaggc gcgcgacatt gtcgccgtag agatggacgg cgatcgcgtc gtcggcgacg
     4801 ccgtacatgt agctgccgat cgaggcggtc aggcgcgcga tgttgggcgg gcagcatggg
     4861 cagcgatgcc atcgccagcg atgatgggcg ccgcggcttt cgagcgggtt ctcgtagaag
     4921 aaccgcgagc cgtcgagcga caggccggaa atcgcgccgt tatagagcgc ccgctccatc
     4981 acgtcggcat agcgggaatc cggccccatt ccgagcatcc ggctcgccca gaagacgagg
     5041 ccgaccgccg cgcatgtctc ggcataggcg gtttcgttgg gaaggtcgta atcgcttgtg
     5101 aagccttcat tatccgccga ggggccgagg ccgccggtga tatagagcct tttgccggtc
     5161 aggtcggccc ataatcgatc gagcgccgct ttcagctcgt cgtcgccata ttcggtcgcg
     5221 atgtcggcca tgcccgaata aaggtacatg gcgcgcaccg cgtggccgac caccttgtcc
     5281 tgttcgcgga ccggcctatg cgactggttg tattcgtagg aattgaaatg aaagtccttc
     5341 gggtcggcgc cgcgggcgcg ggcttcgatg tcgaaatagt gcggctgctg gccgcgctga
     5401 tcgatgaaat atttggcgag atcgagatat ttcctctctc ccgtcgcgcg ggcgagcttc
     5461 accagagcga gttcgatttc ctcgtggccg caatagccct tctttttctc cggctccggc
     5521 ccgaagacgg aagcgatgtg atcggcgtag cggcgcatca cgtcgaggag cttgcgcttg
     5581 ccggtcgcct ggtcataggc gacggcgccc tcgatcagat ggcccgcgca atagagctca
     5641 tggcagtcgc gaaggttggt ccatcttcgg ccgggctgga tgcgttggta ccagctcgac
     5701 agatagccgt ccggttgctg aagcttggcg tacatgtcga tcacggcgtc gattttcgcc
     5761 tcgagcgccg ggttggcctt gcggtagagc gaataggcgg cggtctcgat tgtcttgccg
     5821 agatcggaat cccagaacat ctgggtcgtc acggtcgagg tttcgccgtc gcggatgtgg
     5881 aacggaattt tcacgccagg cgaaggacgg tcgggatcga tctggtcgag catgcccgcg
     5941 gcgacgcagc gctcataaag aatgtcggcg gttcggtccg cgaccgcgtc gatgcggccg
     6001 ccccaaaagc cgccgacctc gacttgcggc gtcgccggcg ggcggaagcg ggcgcgcggc
     6061 ttggaagggg tgggcgctgg cattttctcg aactccatgt gccgtttcgt ctgcgcgcaa
     6121 cgccgcgcca gaaagatgaa gggactgatc ggtgggaaat cgttttccca aagccgcagg
     6181 cgctcccctc gcgggcgcgt aatagagcat gcgccaattc atcagaaaag attttcggcg
     6241 cggcgagggc gcgctctcgg gcatgctcgg cgactcagaa ccgtcgccaa gcagagagtt
     6301 cggcgtaagg accgggccgg cgcccttgtc gccaggacgc gccgatcgac agccgcgcct
     6361 ccatcgaggc gatgagtttg aggcccgtcg catgcaggcc gatccgcggg aagccagcgg
     6421 acgcggcggc ttccggaccg agccacggcc atcgctcatt cgcgcgccag cctgtggcga
     6481 cgcgccatga cgtccaatcg gtcgcgaccg cgacattcgc ttgggccatc caatgcggcg
     6541 tcggctcgat ccacagatcg gcgctggcga gcggatgcgg ccgcggatcg acctcggcgc
     6601 cggccatgaa agtcagggcg aagccgggct gggcgaaacg ccagcccgcc tcgccggcgg
     6661 cgaaaaagcg tcgccaagcc ggcgcgccgc cttccatcga gaagacaagg ccgctcgcgt
     6721 ccaagccgga aaaagcgtgc cgccagccaa cgcccagata gttgcgtccg ctgatcagat
     6781 cgccacagaa atagagaaga tcgttcgcct cgtcggcccg agcgccggcg atgaacgcaa
     6841 ggaaagcgca agcgcagaaa acgagccgca acatgggact gtcccccacg cgcctggccc
     6901 ccgaaggagc gcttcggccg tcccattttc aaagttacgc tgcggttctg gcgggcgaca
     6961 agatatcacg taacgtaagc ggacaatatt ttgccgctcg ggactcaagc cctgatcgcc
     7021 gcgcgcgccg cggcggtgac gtcggcgatg tcggtgaaag ccgtcgcgcc gagcacaagc
     7081 tcacgcccaa tcagcagcgc tttgcgctcg cagaccgccc gcaccgccgg atcggcgatg
     7141 cgctcgagat cctcgacatg ggcgaggccc aggatgcggc ggatcatttt caggccgccg
     7201 aaagcgagcg cgtcttcgaa cagtctccgc atgaagcggg cgcgctcggc gtccatcgcc
     7261 gccgcgtcct gcggcgagac gacgaggctg gcgacatagg cttcgccgcc tttggccttg
     7321 cgccagagcg tgaggaaccg gtcgtgaaag ccggtccaga ccgccgtgac ctggtcgaga
     7381 atccaggcgc gataggcgtc gcgggcgccg ggcgaggctt cgtggccttc ctgcgcgcaa
     7441 aaggcgagga acaggttgcc gatcaccgcg ccgacatcga agccgatcgg gccgacaaag
     7501 gcgaattcgg ggtcgatcac gcgcgtatcc tcggtcgtca gcatgatcga gccggtatgc
     7561 aggtcgccat ggatcagcgc ttgggtcgcg gtgatgaatt ggtacttgcg ggcctgggct
     7621 gcgaatttca gcgcgctgtc cgcctcgaag gcgcgcttgt cggcgtcgag atgcgggcgg
     7681 ttccagcgat tgaggggcgc ctcgcgatag ggatcggtga agacgaggtc ctcggtgatc
     7741 cggcacagtt cggtattggc tgcgaagagc gcaacccttc gcttatgctc gacggcggag
     7801 ccggccagga ccgaggtgtt gaacagggtc tcggcgagaa actcggcgat gtcggcggcg
     7861 aagcgcggat attcgacgcc gcggatcagc cccttgcgca tgatgatatg cggcgagaga
     7921 tattccatca cgatcatcgc ctggtcgcgg tcgaaatgga aaagcttggg cgtcagccgc
     7981 ggcgcgtagc ggctctgttc gacgagcgcg ttatattcga accatgagcg ctccagcggc
     8041 agcggccagg attcgccgac gaggcgcaca tagggcaagg cctgcttgac cacgaccccg
     8101 ccttccgggc cttcgacgat gaagacgaga ttgagattgc cgtcgccgac ctcgcgcacg
     8161 cgccaggcgt cgggctcgcc gccgagccgc gtccgcacat cgggaattcc cgccaaaatc
     8221 gaggaaagct tggcgccgtc gacggccgcg taggcggaag ccatgggtct tgctctcaaa
     8281 gatcgaaatg ctcagccggc gctgcgcgag gcggtgcgca tgcgctcggc gtaaatattg
     8341 atgaccaacg cgatcaaaag gatcaagccg cggatcagga ttttcaggaa gctgtcgatc
     8401 ttcacgtagt caaggccatt attgagcacg ccgagcacga acaggccgac aatcgtgttc
     8461 gggatgccgc cgcgtccgcc gaacagactg gtgccgccga ccacgaccgc ggcgatcgag
     8521 tcgagcagga acgtatcgaa ctcgttctgc tgagcgctgc cgaaataggc gacgccgatc
     8581 atgccggcga cgcccgagca cacggcggaa atgaccatca ctgcgccgac aatcagcttg
     8641 gtgttgacgc cggaatattc cgccgcctcg cggttgccgc cgaccatgta aacgtagcgg
     8701 ccgaaccgcg tataggtcag gacgagatgg gcgacgagga ggaacaaggc cgcgacgatg
     8761 atgatccagg gaacatgcgc gatcgatttc gagccgagcg tctggatcag cgacggcacc
     8821 gtataggcga tctggccgcg caccaggacg gcggaaatgc cggccccgat ctgcatcatc
     8881 gccagcgtca tgatgaagga gggaatgccg atcttggtga cgccgaaggc ggtgaccagg
     8941 ccgagggcga agcaggcgag cagcgcgaag acgatcgcaa cggcgcccgg cagcgggata
     9001 ttggcgatgt tcacgtaatc aggctgcagg gtgaagaagg tcagcacgat cgcggtggcg
     9061 ttggcgatgg cgccgacgga aaggtcgatc tcggcggtca ggatgacgaa ggtgaggccg
     9121 accgcgatga tcccggtcac cgacacctgc tgaagaatgt tgacgagatt gtcgaccgtc
     9181 gcgaaggacg ggctggcgac gctgaagaag gtgacgaggg cgagaagggt gatgaagggc
     9241 gcgatattgc gcaattggtt gcgcaggaaa tcgccgacgc ggaatggcct ctcggcggcg
     9301 acgacattgg ccttgggcgc gtccattgct cctcgctaaa atcaagcggc cgcgagaaga
     9361 cgatccttgc tgatcgcttc gtcggcgaat tcatgggcga tctcgccctt gcgcatcacc
     9421 aggacgcggt ctgcgagcga cagaaccgtc tccggctcgg tcgagaagac gatgatcgca
     9481 atgccctggt cgcgcaaggc gcggacgatt ttgacgacat cctccttcgc gccgacatcc
     9541 atgccgcggg tcggctcggc gaggagcagg atgcgcggcg gatgggtcag ccatttggcg
     9601 agcgcgacct tctgctggtt gccgccggaa agaccgccga gccgcaattc gacattcggc
     9661 gggcggatgt cgagcgcctt gacctgctcg gccgcgagcg cccgctcctg gtcgggcttg
     9721 agcaggaagc gcgacaggcg cgccagcacg gcgatcgaga cgttcttata gaccggctcc
     9781 tcgccaaaca gcatggcgcg ccggctctcg gcgacatagg cgacgccggc cttctgcgcc
     9841 gcgctggtgt tcctaagccg ggccggggcg ccgccgatcg ccagcgagcc gcgatcgggc
     9901 ttgagcttgc cgaacagggt gcgcgccagt tcgagctggc cgcagcccat gaagccgtag
     9961 acgccgagca cctcgccgcc gcgcgcggcg aaggtcacgt cgcgataggc ggcgccgagc
    10021 gtcaggccct tggcttcgag cacgatcggg gcccccggct tgctggccag cctgatctcg
    10081 ccgagatagc tctcctccag ctcctcgcgg ccggcgccga tcatccgctc gatgatccag
    10141 cccttgtcga cctcggcggt caccggcgtg gtcgcgactt tgcggccgtt gcggaaaatc
    10201 gtgatctcgt ccgacacttt taggatgtcg tcgaggaagt gcgaaatgaa gatcaggctg
    10261 cggccgcttt gcttcagctt gaccagcacg tcgaacagcc gctcgatttc cggcggcgac
    10321 agagccgatg tcggctcgtc gagaatgatg atgcgcgcgc ccgagaaaag cacgcgcgaa
    10381 agttcgacga gctgctgcac gccgatcgga aaatcgccga ggcgcgcgcg cggatcgatg
    10441 tcgagaccga gattctgcaa ctgcgccttg gcctgccgcg ccattttgcg ccaggcgacg
    10501 attccgagcc gattgaccgg ctgcatgccg agaaaaacgt tctcggcgac ggtcagctcc
    10561 ggcgcgaccg acaattcctg gtgcaccatg ccgatcccgg cggcgagcgc gtcgcgcgac
    10621 gagcggaaat ggacctcccg cccgtcgacg cgcatcgagc cttcataatc ggcatgcacg
    10681 ccggcgatga ttttcatcag ggtcgacttg ccggcgccgt tctcgccgac gaggccatgg
    10741 atctcgccgg cgttcagcgc gaaatcgacg gcgcgcagag cctgcacgcc gccgaacgat
    10801 ttggtgatgc cggaaagttc aagcagcgcc ggctgcatca gagtttagga ccccaagact
    10861 tgcggctcgc cctcacccca accctctccc gcaagcggga gagggggcgc gccctcacgc
    10921 ttcaaggcgg ggcgctggag ccgcggtcgc cgaccgcggc tccaacccct tctcccgctt
    10981 gcgggagaag gtggccgcga agcggccgga tgagggttcg ccaccaccga atgtacgcca
    11041 gtcgttcaga tcaggaactg atcctccatc cacagcatgc catcggcgtt ggccttggtg
    11101 acgaccgggc cgtcggtgac gatgttcttc gggatgtcgg ttccggtctt ggcgccgttg
    11161 atcaccgccg agacgccggc gatgatcgcg ccgccatgga tgcggcagga gggattgcgc
    11221 accgtggcgt acatctggcc gtcctgcacc gccttgatcg ccggcggcat ggcgtcgacg
    11281 ccgccgatct tggtctcggt gcggcccttg gcctgcatca ccttgtaggc ggcgagcgcc
    11341 atgtcgtcat tgtggaagaa ggccgcgtcg atcttcgggt atttggtcag cagcgtctcc
    11401 cagatgcggg cgaccttggt cacgtcccaa tcggccggct gctcgtcgag cacctggatt
    11461 ttcgggaatt tcgagacgac cgacttgaag ccgcgggccc ggccttgcgc gccggtatgg
    11521 ccaagcgcgc cttgcgtcat gacgatcgtg ccttcgccgt tgatcgcatt gaccagggcc
    11581 tgggtgacgg aggcgcccat gaattcatta tcgggcgcga ggaaggagag gaccttgatc
    11641 tgatcgagcg gcgcgatcag cgtgtccatg tcgatgaccg gaatgccggc gtcgatcatc
    11701 ttgttgaccg gcgcggtgag cgtgccgatg ccgaaagcct ggatggccac gaaatcccat
    11761 ttctgcgagg ccatattgtc gatcgcggag cgctgtttga cggcgtcgag ctgcccgtcg
    11821 aaccaagtca cctcgacgtt gaacaggctg ccccaatatt ccgccgcctg cttgccctgc
    11881 gcacaccagg tcgcttgcag gccggcgttg gagaaggcgg ccttcaaagg cttcgtcgcc
    11941 ttgcccgctt ccttggccat cgcggtggta atgagaggat tgaagccgaa gccgccgaac
    12001 aggctggccg cgccgccgcc gaccgccgcg ctgcggatga gatcgcgcct cgtttgagtc
    12061 ataggttcct ccctgaacat ggcgttgtcg ccatcgatta agcttagcgc ggaagcatga
    12121 catggagggc gcggcgactt caagcggctg ccgccgcgcc gaggcgatgc ggcgcgacgc
    12181 tgcc
//
LOCUS       DATGJW010000501         6571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_199616, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000501 DATGJW010000000
VERSION     DATGJW010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6571
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_199616"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..664)
                     /locus_tag="VKU03_15065"
     CDS             complement(<1..664)
                     /locus_tag="VKU03_15065"
                     /inference="COORDINATES: protein motif:HMM:NF028290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sodium:proton antiporter"
                     /protein_id="HLJ72638.1"
                     /translation="MRLVLILFAFLASTRAAEAAPLDGARLSLGWAAPFLGLLISIGL
                     GPLLAERFWHRHYGKIAFLWAAGLALALAFRVGADAAFAAVAEALIADYLPFILMMFA
                     LYVTAGGVVVRAAAHGRPAINTLLLAIGTVAASAIGVTAASMIMIRPLLHSNYERARK
                     AHVVVFFIFLVANIGGALTPLGNPPLFFGFINGVDFFWPAIHQWPQTLLTVLILLALF
                     FAV"
     gene            complement(841..1656)
                     /gene="dapB"
                     /locus_tag="VKU03_15070"
     CDS             complement(841..1656)
                     /gene="dapB"
                     /locus_tag="VKU03_15070"
                     /EC_number="1.17.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008839 - 4-hydroxy-tetrahydrodipicolinate
                     reductase [Evidence IEA];
                     GO_process: GO:0009089 - lysine biosynthetic process via
                     diaminopimelate [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxy-tetrahydrodipicolinate reductase"
                     /protein_id="HLJ72639.1"
                     /translation="MTDMRLVVTGAAGRMGRMLIKTIVDTKGVVLAAALERENSPALG
                     ADAGALAGLDRLGVPVAADALAAVVNADGIVDFSSPAASVEFAALAAQARIVHVIGTT
                     GLSDDDLKKIEAAARHAAIVRSGNMSLGVNLLAALTRKVAAALGEDADIEIVEMHHRM
                     KVDAPSGTALLLGEAAAAGRGVALKDHQARGRDGLTGARRRGDIGFAALRGGTVIGEH
                     SVIFAADGERIELRHIAEDRSLFARGAVRAALWAQGRKPGLYSMADVLGLDET"
     gene            complement(1874..2179)
                     /locus_tag="VKU03_15075"
     CDS             complement(1874..2179)
                     /locus_tag="VKU03_15075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HLJ72640.1"
                     /translation="MAKGYWIAHVDVSDPEAYKAYTAANAAPFAKYGARFLVRAGQSR
                     QAEGRLRARHVVIEFKDYETALACYNSPEYRAAMALRANASAADLVVIEGYEGPQPG"
     gene            complement(2182..2469)
                     /locus_tag="VKU03_15080"
     CDS             complement(2182..2469)
                     /locus_tag="VKU03_15080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008127436.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1330 domain-containing protein"
                     /protein_id="HLJ72641.1"
                     /translation="MPKGYCIVRVDIHDPEAYALYAKEATDAIKKHGGKVLARGGPYE
                     AMEGQARARNVVVEFDSFEAARAFYLSEEYQRAKKKRERAATVEYVIVEGV"
     gene            complement(2541..3248)
                     /gene="pyrF"
                     /locus_tag="VKU03_15085"
     CDS             complement(2541..3248)
                     /gene="pyrF"
                     /locus_tag="VKU03_15085"
                     /EC_number="4.1.1.23"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012332474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004590 - orotidine-5'-phosphate
                     decarboxylase activity [Evidence IEA];
                     GO_process: GO:0006207 - 'de novo' pyrimidine nucleobase
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0044205 - 'de novo' UMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="orotidine-5'-phosphate decarboxylase"
                     /protein_id="HLJ72642.1"
                     /translation="MSRPLSPRERLIVALDMPRVEEAERLVEELGNVVAFYKVGMELA
                     YGGGLALVSRLAAAGKKVFLDLKLHDIPNTVERATAQVARLGATFLTVHAYPQTMRAA
                     AAGAAGSGLKLLAVTVLTSADDADLAEAGYALGVTALVKKRGAQAKAAGIDGLVASAA
                     EAAALRAELGRDMLLVTPGIRPSGAAPGDQKRVATPAKAIAAGADFLVIGRPIAAAAD
                     PRGAAEAIVAEIGSAAG"
     gene            complement(3444..4250)
                     /locus_tag="VKU03_15090"
     CDS             complement(3444..4250)
                     /locus_tag="VKU03_15090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174343.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribose ABC transporter permease"
                     /protein_id="HLJ72643.1"
                     /translation="MYDPVKRLDRGSGARSRSKHPFVDEAHFIKTLFESPRVTGAVSP
                     SGRFLARAMARAVEARGAGLVVELGPGTGPVTKALIERGVPAEDLVLVEYETSFCRLL
                     AERFPQVRVLQGDAYRLAQSLAGLRGEPIRAVVSSLPLLNEPPSRRLDLIAQAFALMG
                     PRGVFVQFTYGVGSPAPRRVGALTLAAEASAPIWLNLPPARVWTYRVAAAEAAPPRRL
                     RDKASCLADEWAERAEAAARALKVRRDEIGAKARVRAKALAKLRGRTLDF"
     gene            complement(4292..5419)
                     /gene="dnaJ"
                     /locus_tag="VKU03_15095"
     CDS             complement(4292..5419)
                     /gene="dnaJ"
                     /locus_tag="VKU03_15095"
                     /EC_number="1.8.4.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA];
                     GO_process: GO:0009408 - response to heat [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaJ"
                     /protein_id="HLJ72644.1"
                     /translation="MAKRDFYELLGVAKTASDADLKSAFRKAAMQCHPDRNPGDRAAE
                     ARFKELNEAYQTLSDPQKRAAYDRFGHAAFEQGGGFGMGDGFASSMADIFDDLFGDMM
                     GRRGRNASGRERGADLRYNLEITLEEAYRGKAASIRLPTAVACEACAGSGAKPGSKPH
                     ACKTCGGHGRVRAQQGFFAIERTCPTCHGRGQMIDNPCDACAGSGRVTRERNLSVNVP
                     AGVEDGTRIRLSGEGEAGHRGGPPGDLYIFLSIKPHPFFQRDGADLYCRVPVSMVQAA
                     LGGEFVVRTLDGGEATVRIPEGCQSGRQLKLRGKGMPALRSRDIGDLYVQVNVETPQN
                     LTRRQRELLAEFEAESSTKTHPESAGFFSKMKEFFEGLGSS"
     gene            complement(5632..>6571)
                     /locus_tag="VKU03_15100"
     CDS             complement(5632..>6571)
                     /locus_tag="VKU03_15100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012383144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140662 - ATP-dependent protein folding
                     chaperone [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Hsp70 family protein"
                     /protein_id="HLJ72645.1"
                     /translation="GLAAGQIGEVVLVGGMTRMPKVQEIVKQFFGKEPHKGVNPDEVV
                     AIGAAVQAGVLQGDVKDVLLLDVTPLSLGIETLGGVFTRLIDRNTTIPTKKSQIFSTA
                     EDNQTAVTIRVFQGEREMAADNKLLGQFDLVGIPSAPRGVPQIEVTFDIDANGIVNVT
                     AKDKATNKEQQIRIQASGGLSEADINRMVKDAEMHAAEDKKRREMVDVKNRGESLIHD
                     AEKSLQEYGDKVSSADRSAIEAAVAALKSALEGEDVEAIKAKTNDLTQAQMKLGEAMY
                     KAQQASGPEAGAQAAKDDVIDADFKEVGDDDKKKSA"
BASE COUNT         1085 a         2213 c         2211 g         1062 t
ORIGIN      
        1 cgacggcaaa gaaaagcgcc agcaaaatca agacggtcaa aagcgtctgc ggccattgat
       61 ggatggcggg ccagaagaaa tcgacgccgt tgatgaagcc gaagaagagc ggcggattgc
      121 cgagcggcgt cagcgcgccg ccgatattgg cgacaagaaa gatgaagaag acgacgacat
      181 gcgccttgcg cgcgcgctcg tagttcgaat ggagcagcgg ccggatcatg atcatcgacg
      241 ccgcggtgac gccgatggcg ctcgccgcga ccgtgccgat cgccagcagc agcgtgttga
      301 tcgccggccg gccatgcgcg gcggcgcgta caacgacgcc gcccgccgtc acgtagagcg
      361 cgaacatcat caggatgaac ggcaggtagt cggcgatcag cgcctccgcg acggcggcga
      421 aggcggcgtc cgcgccgacg cggaaagcga gcgcgagagc aaggccggcc gcccagagga
      481 aggcgatctt gccatagtga cgatgccaga agcgctcggc gagaagcggg cctaacccga
      541 tcgagatcag caagccgaga aaaggcgcgg cccagcccag tgaaagccgc gcgccgtcga
      601 gcggcgcggc ctcggccgcg cgcgtcgagg cgaggaaggc gaagaggatg aggacaaggc
      661 gcaaacccct ctcccggacg gagaggggta ggggcgaggg gtacggcctc tcgagaaagg
      721 caaaggtcgc cgcagcgccg aaagtacgac ctcggaaatc ccaagccgcg gtccgctcgc
      781 gcccagtcct tctcccttcg ggagagaggg aagcgcctgc gccgccgccg gccgccgccc
      841 tcacgtctcg tccaggccca gcacgtcggc catggaatag agacccggct tgcgcccttg
      901 cgcccagagc gccgcccgca cggcgccgcg cgcgaacagc gagcggtctt cggcgatgtg
      961 ccgcaattcg atccgctcgc cgtcggcggc gaaaatcacg ctgtgctcgc cgatcacggt
     1021 gccgccgcgc agggcggcga agccgatatc gccgcgccgc cgcgccccgg tcaggccgtc
     1081 gcggccgcgc gcctgatgat ccttcagcgc gacgccgcgg ccggcggcgg ccgcctcgcc
     1141 gagcagaagc gcggtgccgg acggcgcgtc gaccttcatc cggtgatgca tttcgacgat
     1201 ttcgatgtcg gcgtcctcgc cgagcgcggc ggcgaccttg cgcgtcaagg cggcgaggag
     1261 attgacgccg aggctcatat tgccggagcg gacgatcgcg gcatggcgcg ccgccgcctc
     1321 gatctttttc agatcgtcgt cggagagccc ggtcgtgccg atgacatgga cgatgcgcgc
     1381 ctgcgcggcg agcgcggcga attcgacgct ggccgcgggc gaggaaaaat cgacaatgcc
     1441 gtcggcgttg accaccgcgg cgagcgcgtc ggcggcgacg gggacgccga gccggtcgag
     1501 cccggccagc gcgccggcgt cggcgccgag cgccggcgag ttctcgcgct ccaaagcggc
     1561 ggcgaggacg acgcctttcg tgtcgacgat cgtcttgatc agcatccgcc ccatgcgtcc
     1621 ggcggcgccg gtcacgacga gccgcatatc ggtcatagat tgctccaaca gaatcccatc
     1681 ggccgctacc ggaacttgcg gcgcaccctc gccccaaccc tctcccttaa agtgggagag
     1741 agggcgcgcc gcgccttcat cggctggcgc tgtagccgcg gtcgccgacc gcggctacaa
     1801 tcccttctcc cgctttaagc gagaacgtgg ctcgagggat agacgcggcg ttgacgatcc
     1861 cgattccatc gtctcagcct ggctgcggcc cttcatagcc ttcgatgacg acgaggtcgg
     1921 cggcgctggc attggcgcgc agagccatgg cggcgcgata ttccggcgaa ttgtagcagg
     1981 cgagcgcggt ttcatagtcc ttgaattcga tcacgacatg gcgggcgcgc agacgcccct
     2041 ccgcctgccg gctctgcccc gcccggacga gaaagcgcgc gccgtatttc gcgaagggag
     2101 cggcattggc ggcagtatag gctttgtacg cctccggatc gctcacatcg acatgcgcga
     2161 tccaataacc cttggccatg ctcacactcc ctcgacaatg acgtattcga ccgtcgcggc
     2221 gcgctcgcgc ttcttcttgg cgcgctgata ttcctcggag aggtagaagg cgcgcgcggc
     2281 ctcgaaactg tcgaactcga caacgacatt gcgcgcacgc gcctgtccct ccatcgcctc
     2341 ataggggccg ccgcgcgcca gcaccttgcc gccatgtttc ttgatcgcgt cggtcgcctc
     2401 cttggcgtaa agcgcatagg cttccgggtc atgaatatcg acccgaacga tgcaatagcc
     2461 ctttggcatg gcgttcctct cttctcgcga atggcgagtg ttctaaggca atatcgaacc
     2521 ggagcaaatc gcggacgggc tcagccggcc gccgaaccga tctcggcgac gatggcctcg
     2581 gcggcgccgc gcggatcggc ggccgcggcg atcgggcggc cgatgacgag aaaatcggcg
     2641 ccggcggcga tggctttggc cggcgtcgcg acccgcttct gatcgcctgg ggcggcgccg
     2701 ctcgggcgaa tgcccggcgt gaccagaagc atgtcccggc cgagttcggc gcgcagcgcc
     2761 gccgcttccg ccgcgctggc gaccagtccg tcgattcccg ccgccttggc ttgtgcgccg
     2821 cgcttcttga ccagcgccgt cacgccgagc gcatagccgg cctcggcgag gtccgcgtcg
     2881 tcggcggagg tgagaacggt gacggcgagc agcttcagtc cgctccccgc cgcgccggcg
     2941 gcggcggcgc gcatcgtctg cgggtaggcg tgaacggtca ggaacgtcgc gccgagccgg
     3001 gcgacctgcg cggtcgcgcg ctcgaccgtg ttgggaatgt cgtgcagctt gagatcgaga
     3061 aaaaccttct tgccggcggc ggcgaggcgc gagaccagcg ccagcccgcc gccataggcg
     3121 agctccatgc cgaccttata gaaggccacg acattgccga gctcctcgac cagccgctca
     3181 gcctcttcga cgcgcggcat atccaaggcg acgatcaggc gttcgcgggg ggacagtgga
     3241 cgagacatcg caggctcgct ttttcgttga cgtactcaat agcaacttgc ttgcgccgag
     3301 atttaccttc atccgggcgc ttcgcggccg ccttctcccg caagcggaag aagggaaaga
     3361 ggggaggccg ctgacaggct cggcgccctc tctctcccgc ttgcgggaga aggtttggat
     3421 aagcgccagc acgcggctcg acttcaaaaa tccaaagtac gtccgcgcag cttggcgagc
     3481 gccttggcgc ggacgcgggc tttggcgccg atttcgtcgc ggcgcacttt cagcgcccgc
     3541 gccgccgctt ccgcgcgctc ggcccattcg tcagccaagc agctcgcctt gtcgcgcaaa
     3601 cggcgcggcg gcgcggcttc ggcggcggcg acgcggtagg tccagacccg ggcgggcggc
     3661 agattgagcc agatcggcgc gctcgcctcg gcggccaaag tcagcgcgcc gacgcggcgc
     3721 ggcgccggcg agccgacgcc ataggtgaat tgcacgaaga cgccgcgcgg ccccatcagg
     3781 gcgaaagcct gcgcgatcaa gtcgagccgg cgcgacggcg gctcgttgag gagcggcagg
     3841 ctcgacacga cggcgcggat cggctcgccg cgcagaccgg cgaggctttg cgccaggcga
     3901 taggcgtcgc cttgcaggac ccgcacttgc ggaaagcgct cggcgaggag gcggcagaag
     3961 ctggtctcgt attcgacgag aaccaggtct tcggccggca cgccgcgctc gatcagcgcc
     4021 ttggtgactg gcccggtgcc cggcccgagc tcgacgacaa ggcccgcccc ccgcgcctcg
     4081 acggcccggg ccatggcgcg ggcgagaaat cgtccggacg gcgagacggc gccggtgacg
     4141 cgcgggcttt cgaacagggt cttgatgaaa tgggcttcat cgacaaaggg atgcttggaa
     4201 cgcgagcgcg cgccggagcc ccgatcgagc cgcttcaccg gatcgtacac gaaagcctct
     4261 taaattgtcg gcgtcccggc gctcttcggc gtcaagaact gccgagcccc tcgaagaatt
     4321 ccttcatttt ggaaaaaaag ccggcgcttt ccggatgagt tttcgtcgat gattcggctt
     4381 cgaactcggc caacaattcg cgctggcggc gcgttaaatt ctgcggcgtc tcgacattaa
     4441 cctgcacata gaggtcgccg atgtcgcggg agcgcaaggc cggcatgccc ttgcccctga
     4501 gcttcaattg ccggccggac tggcagcctt cgggtatgcg caccgtcgct tcgccgccgt
     4561 ccaaggtgcg cacgacgaat tcgccgccca acgccgcctg gaccatcgaa accggcaccc
     4621 ggcagtaaag atcggcgccg tcgcgctgaa agaagggatg cggcttgatc gacaggaaaa
     4681 tatagagatc gccgggcggg ccgccgcgat ggccggcctc cccctcgccg gaaaggcgga
     4741 tgcgggtgcc gtcctcgacc ccggccggga cattgacgga aaggttgcgc tcgcgggtta
     4801 cccggcccga gccggcgcag gcgtcgcagg gattgtcgat catctggccg cggccatggc
     4861 aggtcgggca ggtccgctcg atcgcgaaaa agccctgctg ggctcgcaca cggccatggc
     4921 cgccgcatgt cttgcaggca tgcggcttgg agccgggctt ggcgccggag ccggcgcagg
     4981 cttcgcacgc gaccgcggtc ggcagccgga tcgaggcggc cttgccgcga taggcttctt
     5041 cgagggtgat ttcgagatta tagcgcagat cggcgccgcg ctcgcggccg gaggcgttgc
     5101 ggccgcgccg gcccatcatg tcgccgaaca gatcgtcgaa tatgtcggcc attgacgagg
     5161 cgaagccgtc gcccatgccg aaaccgccgc cctgctcgaa cgcggcatgg ccgaagcgat
     5221 cgtaggcggc gcgcttctgc ggatcggaca gagtctggta cgcctcgttc agttccttga
     5281 agcgcgcttc ggcggcgcga tcgccgggat tgcggtccgg atggcactgc atcgccgctt
     5341 tgcggaaggc cgatttgaga tccgcatcgg aagcggtctt ggcgacgccc aacaattcgt
     5401 agaagtcgcg tttggccacg tctgcttcag cccggttcat cgctcaaggc gcctcgccgc
     5461 ggccgcgcgc cgccgcgacc cgaagtccgc cccgtcctaa gcgtccatcc atttcagata
     5521 ttcgctccaa tggaaaggtg gaagtcgccg ctctcccgcc tcggcgcggc ggatgcggcg
     5581 ccgggctcag caacgaagga cggcggcttt gagccgccgt ccttacaaag atcaggccga
     5641 tttctttttg tcgtcgtcgc caacctcttt gaagtcggcg tcgatcacat cgtccttggc
     5701 ggcttgcgcg ccggcctcgg gcccgctcgc ctgctgcgcc ttgtacatcg cctcgccgag
     5761 cttcatctgc gcctgcgtga gatcgttggt cttggccttg atcgcctcga catcctcgcc
     5821 ttcgagcgcg ctcttcagcg cggcgacggc cgcctcgatc gcgctgcgat cggccgacga
     5881 aaccttgtcg ccatattcct gcagcgactt ctcggcgtcg tggatcagcg attcgccgcg
     5941 gttcttcacg tcgaccatct cgcgccgctt cttgtcctcg gcggcgtgca tctcggcgtc
     6001 cttgaccatc cggttgatgt cggcctcgct gaggccgccg gaagcctgga tgcggatctg
     6061 ctgctccttg ttggtcgcct tgtccttcgc cgtgacattg acgatgccgt tcgcgtcgat
     6121 gtcgaaggtc acttcgatct gcggcacgcc gcgcggcgcg gagggaatgc cgacgagatc
     6181 gaactggccg agcagcttgt tgtccgccgc catttcgcgc tcgccctgga agacccggat
     6241 cgtcaccgcc gtttgattgt cctcggcggt cgagaaaatc tggctcttct tggtcgggat
     6301 cgtcgtgttg cgatcgatca gccgggtgaa gacgccgccc agagtctcga tgccgagcga
     6361 cagcggcgtc acgtcgagga gcagaacgtc cttgacgtcg ccctgcaaaa cgccggcctg
     6421 gaccgccgcg ccgatcgcga cgacttcgtc cggattgacg cccttgtgcg gctccttgcc
     6481 gaagaactgc ttaacgatct cttgaacctt gggcatgcgc gtcatgccgc cgacgagaac
     6541 gacctcgccg atctggccag ccgccaggcc c
//
LOCUS       DATGJW010000502         5019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_505832, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000502 DATGJW010000000
VERSION     DATGJW010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5019
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_505832"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(46..450)
                     /locus_tag="VKU03_15105"
     CDS             complement(46..450)
                     /locus_tag="VKU03_15105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130568.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72646.1"
                     /translation="MRREVSLFRFAGATAALALLATSLAGPAWAAPSPTAGFGSLDRD
                     LSLAQWQKPGPGKPGGKPGWKPGPGHGPGGGHWRHGGGGGHWRNGVWVPALAFGVLGA
                     AAAAAAFGAPPQPGMCWYYDDPFRTTGHWDYC"
     gene            complement(532..753)
                     /locus_tag="VKU03_15110"
     CDS             complement(532..753)
                     /locus_tag="VKU03_15110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019862056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4-oxalocrotonate tautomerase family protein"
                     /protein_id="HLJ72647.1"
                     /translation="MPFVNIRLVKEVIAADPSGKKAAIAKKVTAAITEATGLGADDVW
                     VVFEEVAARDWFVAATSVEERRKARAAAS"
     gene            complement(831..1544)
                     /locus_tag="VKU03_15115"
     CDS             complement(831..1544)
                     /locus_tag="VKU03_15115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012592268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cell cycle transcriptional regulator TrcR"
                     /protein_id="HLJ72648.1"
                     /translation="MSEAPLMPKATAVWLVENTSLTFDQIAEFCKLHPLEVKGIADGE
                     VATGIKGHDPIASGQLTREEIAEGEKNPERRLALAVAKVRLPEFKKPRGARYTPLSRR
                     QDRPNAILWLLRNHPELKDAQIMRLVGTTKSTLQAIRERSHWNSANLQPMDPVTLGLC
                     SQIDLDFEVNRAAKERPKVEEEGGQTLVPAEITTAKPAPAPTTRADVFGAPAKPKSEE
                     EKIDVDSVFGKLKTLKNAG"
     gene            complement(1717..2346)
                     /gene="upp"
                     /locus_tag="VKU03_15120"
     CDS             complement(1717..2346)
                     /gene="upp"
                     /locus_tag="VKU03_15120"
                     /EC_number="2.4.2.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006614847.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004845 - uracil phosphoribosyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil phosphoribosyltransferase"
                     /protein_id="HLJ72649.1"
                     /translation="MPAAHVVDHPLVQHKLTLMRDKRRSTKGFRQLMNEIGMLLCYEV
                     TRDLPIEMVEVETPLEKTMKPMIAGKKLVFAPILRAGVGFLDGMLELVPSARVAHIGI
                     YRDPQTLEAVEYYFKAPADVAERLVIVMDPMLATANSAIAAIDRLKRRGVKDIRFVCL
                     LAAPEGVERLSKAHPEVEIWTAAIDERLNDHGYIVPGLGDAGDRMFGTR"
     gene            2598..3191
                     /gene="pgsA"
                     /locus_tag="VKU03_15125"
     CDS             2598..3191
                     /gene="pgsA"
                     /locus_tag="VKU03_15125"
                     /EC_number="2.7.8.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003610489.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008444 -
                     CDP-diacylglycerol-glycerol-3-phosphate
                     3-phosphatidyltransferase activity [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-diacylglycerol--glycerol-3-phosphate
                     3-phosphatidyltransferase"
                     /protein_id="HLJ72650.1"
                     /translation="MASASTGAAPRQWTLPNLLTYGRLVAVPLVVALLFWPAEHWMRW
                     TALAIFVAAAITDFLDGYLARAWSQQSSLGRMLDPIADKLLVAATLLVLAADKVIAGW
                     SLWAAIVILCREILVSGLREFLAELRVPVPVSTIAKWKTTVQLVALGFLIAGPAGEQL
                     LPGTTQIGVILLWAAAILTLYTGWDYLKASFDHLASG"
     gene            3199..3450
                     /gene="moaD"
                     /locus_tag="VKU03_15130"
     CDS             3199..3450
                     /gene="moaD"
                     /locus_tag="VKU03_15130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018648370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin converting factor subunit 1"
                     /protein_id="HLJ72651.1"
                     /translation="MKALYFAWLRERVGKSEEEIAPPAGVATIGELIAWLSAKDEAYA
                     YAFEKKNVIRAAIDHAHVSHDAKIAGAKEIAFFPPMTGG"
     gene            3453..3914
                     /locus_tag="VKU03_15135"
     CDS             3453..3914
                     /locus_tag="VKU03_15135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920506.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HLJ72652.1"
                     /translation="MKAAPIIRVQREDFDAAAEADSLRKGRADIGALVSFVGLCRDEG
                     GRLEALEIEHYGAMALAEIERVAGEALTRWPLLGLTIVHRYGLIEAGGRIVLVAAAAA
                     HRGEAFEAANFLMDYLKTRAPFWKRAHLVGGGLGDWIEPKSADDEAAARWN"
     gene            complement(4027..4275)
                     /locus_tag="VKU03_15140"
     CDS             complement(4027..4275)
                     /locus_tag="VKU03_15140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004688036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GlsB/YeaQ/YmgE family stress response membrane
                     protein"
                     /protein_id="HLJ72653.1"
                     /translation="MGIIAWIILGLIAGFIASKIVNKTGEGFLMDIALGVIGALVGGF
                     LFSLIGHQGVTGLNVWSLFVSVIGAIVVLLVYRAVVKA"
     gene            4486..4929
                     /locus_tag="VKU03_15145"
     CDS             4486..4929
                     /locus_tag="VKU03_15145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318001.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YidB family protein"
                     /protein_id="HLJ72654.1"
                     /translation="MGLFDDALRGAVPGGNVAAPLAIAAGALLLGKYFSNRSAAPAQP
                     AAPAAPQGGGLLSGLNDLVQKFQNAGHGEAVNSWVGTGPNQPVSPSQVGSALGQQTIS
                     DLARQAGVSEQELLSQLSLVLPSLVDKLTPNGRLPTQAELAPHGY"
BASE COUNT          826 a         1639 c         1641 g          913 t
ORIGIN      
        1 actcaaacct caaaaaaagc ggcgcttggc gcgccgcttc tagggctagc aatagtccca
       61 gtgtccggtg gtccggaacg gatcgtcgta ataccagcac atgccgggct ggggcggcgc
      121 gccgaaggcc gcggcagcag cggccgcgcc aagcacgcca aaagcgagag ccggcaccca
      181 gacgccgttg cgccaatggc cgccgcctcc gccatgacgc cagtggccgc cgccgggccc
      241 atggcctggg ccgggcttcc agccgggttt gccgccaggc ttgccggggc cgggcttctg
      301 ccattgcgcc agggacagat cgcggtcgag cgagccgaag ccggcggtcg gcgacggcgc
      361 ggcccaagcc ggcccggcaa gggacgtcgc cagcagggcg agggcggccg tcgcgccggc
      421 gaaccgaaac aaagaaactt cccgtctcat ggagcgctcc ttgcaaaaat gccccgagac
      481 agttgagcgt ggaagcggcg gggcgtcaac ccgcggccgg gcggccgcga tttaggaggc
      541 ggcggccctg gctttgcgtc tctcctcgac gctggtcgcc gcgacgaacc aatccctcgc
      601 cgcgacctct tcaaaaacga cccacacgtc gtcggctccg aggccggtcg cttccgtgat
      661 cgcggccgtc actttcttgg cgatcgcggc ctttttgccg gacggatcgg cggcgattac
      721 ttccttgaca aggcggatgt tgacgaacgg catttcggcc ctccctactg gcgtcctgcg
      781 ccttcgcccc gccggttgtc gcgtccgaag gggccattgt cgccggcctt tcagccggcg
      841 tttttgagcg tcttgagctt gccgaagacc gaatccacgt cgatcttttc ttcttcgctc
      901 ttgggcttgg ccggcgcgcc gaacacgtcc gcccgggtcg tcggcgccgg ggccggcttg
      961 gcggtggtga tttcggccgg caccagggtc tggccgcctt cctcctccac tttcggccgc
     1021 tccttggcgg cgcgattgac ctcgaaatcg agatcgatct gcgagcagag gccgagcgtc
     1081 accgggtcca tgggctgcaa attggccgag ttccaatggc tgcgctcacg gatggcctgc
     1141 agcgtcgatt tggtcgtgcc gaccagccgc atgatctgcg cgtccttcag ctcgggatgg
     1201 ttgcgcaaca gccagagaat ggcgttgggc cggtcctggc gccgcgacag gggcgtgtag
     1261 cgggcgccgc gcggtttctt gaattccggc aggcgcacct tggcgacagc cagcgccagc
     1321 cggcgctccg ggttcttctc accctcggcg atttcctcgc gggtcaattg gccggaggcg
     1381 atcgggtcat gacccttgat gccggtcgcc acctctccgt cggcgattcc tttcacttcc
     1441 aacggatgga gtttgcagaa ttcggcaatt tgatcgaagg tgagcgaggt gttttcgacc
     1501 agccagacgg cggtcgcttt gggcattagc ggcgcttcgc tcatagtttc ggttctcctt
     1561 cgcctgtccc caagaataca tcgcggcgcg ctttcttagc cagcgcccgg gaggcggagc
     1621 ctcaagtcat catccatgcg ggaatggccc gatatatagg cgcggccaag cgggaaggca
     1681 attggaaggg cgatcaattg tcgggcgcgg ccgccctcat ctcgtcccga acatccggtc
     1741 gccagcgtcg ccgaggcctg gcacgatata gccgtgatcg ttgaggcgct cgtcgatcgc
     1801 ggcggtccag atttcaacct cgggatgagc cttgctcagt ctctcgaccc cttccggggc
     1861 cgccagaagg cagacaaagc gaatgtcctt gaccccgcgg cgcttgaggc ggtcgatcgc
     1921 agcgatcgcc gaattggcgg tcgccaacat cgggtccata acgatgacca ggcgctcggc
     1981 gacgtcggcc ggggccttga agtaatattc caccgcctcc agggtttgcg ggtcgcggta
     2041 aatgccgata tgggcgaccc gggccgaggg cacgagctcc agcatgccgt cgagaaagcc
     2101 gacgccggcg cgcaggatcg gcgcgaagac caatttcttg ccggcgatca tcggcttcat
     2161 cgtcttttcg agcggcgtct cgacctcgac catctcgatc ggcagatcgc gcgtcacctc
     2221 gtagcagagc agcatgccga tctcgttcat gagctggcgg aagcccttgg tcgagcgccg
     2281 cttatcgcgc atcagcgtca gcttgtgctg gaccagggga tggtcgacga catgcgcggc
     2341 gggcataggg gcctcgaaat cggcagtagg cagtcggcag tgggcagtcg gaaacggcaa
     2401 taggcaggag ccggcgagcg ctggcaaggc cggcggcggg ttcagcgccc tttacccacc
     2461 gcctcgacgc cgactgccga ctgccgattg ccgactgccg attttcctct cccgccttta
     2521 ttccgtccgg cctcgcccct atgtgacatt cgtggtcatt gaccgcgacc gctcgacaat
     2581 gtcacctcct gagggtcatg gcttctgctt ccaccggcgc cgcgccgcgg caatggactc
     2641 tgccgaactt gctgacctat ggtcggttgg tcgcggtgcc gctcgtcgtc gcgcttttgt
     2701 tctggccggc ggagcattgg atgcgctgga cggcgctggc gatttttgtc gccgcggcga
     2761 tcaccgattt cctcgacggc tatctcgccc gcgcctggtc gcagcaatcc tcgctcggcc
     2821 gcatgctcga tccgatcgcc gacaagttgc tggtcgcggc gaccttgctg gtgctcgccg
     2881 ccgacaaggt catcgccggc tggtcgctgt gggcggcgat cgtgatttta tgccgcgaaa
     2941 tcctggtctc gggcctgcgc gaatttctcg ccgaattgcg ggtgccggtg ccggtctcga
     3001 ccatcgccaa atggaaaacg acggtgcagc tcgtcgccct cggcttcctg atcgccggtc
     3061 cggccggcga gcagctcctt cccggaacga cccagatcgg cgtcatcctc ctctgggcgg
     3121 cggcgatcct gacgttgtac acggggtggg attatctgaa ggcgagcttc gatcatctcg
     3181 cctcgggctg agcggacgat gaaggcgctt tatttcgcct ggctgcgcga gcgcgtcggc
     3241 aaaagcgaag aagagatcgc gccgcccgcc ggcgtcgcga cgatcggcga actcatcgct
     3301 tggctctcgg cgaaggacga ggcttatgcg tacgccttcg aaaagaaaaa cgtgatccgg
     3361 gcggcgatcg accacgccca tgtctcgcat gacgcgaaaa tcgccggggc caaggagatc
     3421 gccttctttc ccccgatgac cggcggctga cgatgaaagc cgcgccgatc attcgcgttc
     3481 agcgcgagga tttcgacgcc gccgcggagg ccgattcttt gcgcaaaggc cgcgccgata
     3541 tcggcgcgct ggtcagtttc gtcggcctgt gtcgcgacga gggcgggagg cttgaagcgc
     3601 tcgaaatcga gcattacggc gccatggcct tggccgagat cgagcgcgtc gccggcgagg
     3661 ccttgacgcg ctggccgctt ctcggcctga cgatcgtcca tcgctacggt ctgatcgagg
     3721 cgggcggccg catcgtactc gtcgccgccg ctgcggccca tcgcggcgaa gcgttcgaag
     3781 cggcgaattt cctgatggat tatctgaaga cgcgcgcgcc cttctggaag cgcgcccatc
     3841 tcgtcggcgg cgggctcggc gattggatcg agccgaaaag tgcggacgat gaagccgcgg
     3901 cgcggtggaa ttgaactcgc ctgtcacttc cgcgaagtgg gaacccagca cgacctctct
     3961 ttcgatacgg cggttctctg gattcccgct tgcgcgggaa tgagggcggc gccccgcgcc
     4021 gacaattcag gctttcacga cggctcgata gacgagaagc acgacgatcg cgccgatcac
     4081 cgagacgaac aggctccaca cattgaggcc ggtgacgccc tgatggccga tgaggctgaa
     4141 caggaatccg ccgaccagcg cgccgatcac gccaagggcg atatccatca gaaagccttc
     4201 gccggtcttg ttaacgatct tgctggcgat gaatccggca atgagtccga gaatgatcca
     4261 ggctatgatt cccataatat ccacccctct gctggcgagc gccgcgctcg gctaaaaggc
     4321 gtatcgagtg cgccgacccg atacggcggc ggagcatccg cgatggacgc ataaaaggca
     4381 aggcggtatc gtcgcgccag gacgccttgc gagcggcgca tcgcgaaatc gtcgccgctg
     4441 acgcagcgtt gtggcgcgtt ccaaactgtt catgaggagg tcgctatggg acttttcgac
     4501 gacgctttgc ggggcgctgt gcccggcggc aatgtcgccg cgccgctcgc catcgcggcc
     4561 ggcgcgctcc tgctcggcaa gtatttcagc aatcgctctg cggcgccggc gcagcctgcc
     4621 gcgcccgccg cgccgcaagg cggcgggctg ctcagcgggc ttaacgatct cgtgcaaaaa
     4681 ttccagaacg ccgggcatgg cgaggcggtc aattcctggg tcggaaccgg acccaatcag
     4741 ccggtctcgc cttcccaggt gggctcggcg ctcggccagc agacgatcag cgatctcgcc
     4801 cgccaggccg gcgtcagcga acaggaattg ctcagccagc tttcgctggt cctgcccagc
     4861 ctggtcgaca agctgacgcc caacggccgc ctgccgacgc aagccgaact cgcgccgcac
     4921 ggttattgag ggacgcaggc gcacgcatcc cttctccccg caaagcgcag ggctgtccgg
     4981 ggaaaataac gggcggatcg aatccggcgc acgggtatt
//
LOCUS       DATGJW010000503        14428 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1028282, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000503 DATGJW010000000
VERSION     DATGJW010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 14428)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14428)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14428
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1028282"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(312..2624)
                     /locus_tag="VKU03_15150"
     CDS             complement(312..2624)
                     /locus_tag="VKU03_15150"
                     /inference="COORDINATES: protein motif:HMM:NF024267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ72655.1"
                     /translation="MARANAANGDMRAAGWRNFAAATAHSLRFRSPLQVRLWLLYVAP
                     ALAALIFAAATLTIAAQMRLERERALRAAEHEVGLRASLLAFRLNEALAANPAGAPAD
                     LLRVALGGDEALREGDALIAGADGGLSASEPPAAGANLAAVLGPGPLAIFGEKAGVLR
                     IVTADGADAFATVRSLRPPLAQLAYIMRAEGLLASWRASAIRAALLLTAAALLLVACF
                     AVPYRQAARARRIETLARSARNRVDSALNRGRCGLWDWDLSRGRIQWSRSMYELLDLP
                     PSLDLLSIGDVQAMVHPEDGDLSAIAQGVIDGEQPTVDHEFRLRNAAGDWVWLRARAE
                     LVSDSSSGRHLVGVALDVTEQKALAEISATADQRLREAIEAISEAFVLWDSSNHLVLC
                     NSKFQRLYNLPPEAVRPGVPYAELAAKGAPPMIRNEVALRASESGEAGKPGAYEARLT
                     DGRWLQINERRTKDGGYVSVGTDITALKRHEEQLMDSERRLLASVAELRRSRQALEAQ
                     TQQLADLAERYLEQKAQAESANRAKAEFLANMSHELRTPLNAIIGFSEMMSQEAFGPL
                     GSPKYHDYCSHIRDSGRYLHNVISDVLDMSRLEAGRVRLNRSNLAVETAIRNSVHQVA
                     EAARDKKLSIEVDVNPTEIVYADPKAIERVLVTLLRNAVKFTPDGGEISIGAQAFDEQ
                     IYIYVEDTGPGIPAADLSRLGRPFEQAEVTMANGMKGSGLGLAIATSLVELHGGSLRI
                     NSRPGDGAVVLVALPRSPREHHKLALAVGA"
     gene            complement(2934..5588)
                     /gene="pepN"
                     /locus_tag="VKU03_15155"
     CDS             complement(2934..5588)
                     /gene="pepN"
                     /locus_tag="VKU03_15155"
                     /EC_number="3.4.11.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402083.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase N"
                     /protein_id="HLJ72656.1"
                     /translation="MRTTTGQPIRLSDYRPPDYLIDDVRLDIALDRRAARVVSTLKLR
                     PNPAGRANVALDLDGDELRLLSVSLDGAPLDAGAFEATPDKLTLPNPPRRPFTLRIET
                     ELDPSANTKLMGLYRSGSAYCTQCEAEGFRRISYFLDRPDVLSTYVTRLEADKKEAPI
                     LLANGDLEETGEAADGRHYAVWRDPYKKPCYLFALVAGDLAHIEDRFVTRSGREVKLA
                     IYVEHGKEDRAYYAMDSLKRAMAWDERVYGREYDLSVFNIVAVSDFNMGAMENKGLNV
                     FNDKYVLALPETATDDDYAGVEGVIAHEYFHNWTGNRITCRDWFQLCLKEGLTVFRDQ
                     DFSADQRSAPVKRIADVRGLRAAQFPEDAGPLAHNVRPEVYHEINNFYTATVYQKGAE
                     VIRMLKLLIGADAFRRGMDLYFERCDGTAATVEDFLQCFADVSGRDLREFKRWYSQAG
                     TPLLTVRGAYDAAARTYTLDLTQATPPTRDQAVKAPVVIPIALGLVAPTGEEIALSSG
                     DATASETARGVFELNAASRRIVFKNVPARPALSLLRGFSAPVRVDDDLTESDLLILSR
                     HDRDSFNRWQAVQSLATRVLLRAVEAIRAGRPAEIETGFSAAYGGVLADALDGKLDFA
                     FAALTATLPSEADLARETAKNVDPDAIFAAREFLRASLGRAHAAALAQIYDKLFDAGP
                     FSPDARSAGRRALRNLALAMLTAGREPGCVERAQRQFAEANNMTERFAALAALAAIPG
                     EAREHALESFWRAHADDALILDKWFALQAQIPERETLDRVRGLMAHHSFSLSNPNRVR
                     ALIGGFAANQTQFNRADGLGYELLAEVVLKLDPSNPQIAARLLSALRSWRSFEPKRQE
                     RAEATLRRIAAQAGLSADLSDIVTRSLS"
     gene            complement(5659..6522)
                     /gene="murI"
                     /locus_tag="VKU03_15160"
     CDS             complement(5659..6522)
                     /gene="murI"
                     /locus_tag="VKU03_15160"
                     /EC_number="5.1.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008881 - glutamate racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate racemase"
                     /protein_id="HLJ72657.1"
                     /translation="MRILVFDSGLGGLTVYEQIAASRASDELFYAADDAAFPYGALAE
                     QALVERVVAIVGELIAALAPDLVVIACNTASTLALGPLRARHPALPFVGTVPAIKPAA
                     AITRSRLISVLGTPGTVARDYTQALAREYAAGCEVKLVGAPDLARLAEAHIKGLAVAD
                     EAIAKEIAPCFVARKGRRTDVVVLACTHYPLLAERLAALAPWPVDYIDPAPAIARRVD
                     HLLGPAEALAKRADRLAPAIFTSGAPPSPALAEALKRFSLEVRTLPEMTAALKPRSPT
                     AASARGLTRLA"
     gene            complement(6539..7960)
                     /locus_tag="VKU03_15165"
     CDS             complement(6539..7960)
                     /locus_tag="VKU03_15165"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HLJ72658.1"
                     /translation="MAIFMRPGPRLAFLIGAAAFLIGGDAAFAAQNASPPAAGAADQV
                     FQAQKAAYEALSEADRRAIQDALIWTGDYNGVVDGNFGKRTRDAILAYQADAKTPTTG
                     ILDPAALARLIAVAGKAKAAVKFQIVADDKSGVRIGAPLRLLDRRAALPNGSRLANAE
                     GSATLDLVSAGGDLAALYARLSADTPGRKTTLKLSRPDFFVLSGEEGGRKFYLRYAKA
                     PTNWPDPSQVRGFKLTYPAQSASFDRIAVAIADTFEPFPAAATPAANATTVPAKAPTP
                     SKPALAANGLIVAAGEALTALDSGACPHPSVDGKPAKYLREDKQAGLSLIGAGFAGKP
                     AAAPLAVAPLSDDLVALTYAADPAGAKPALQVAAIAPLAGATDAAKPRLLASLPQNAA
                     GSPVFDRSGALVAIVAPAAAEAKPVAGVIPLAPHPVVAASDIERFLAGADIALAKATE
                     AGASAAGRIAAEKGALVVPIVCD"
     gene            8125..13368
                     /locus_tag="VKU03_15170"
     CDS             8125..13368
                     /locus_tag="VKU03_15170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0010951 - negative regulation of
                     endopeptidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin"
                     /protein_id="HLJ72659.1"
                     /translation="MRLWLSSLLIIVVGAFAAGAQAAEIKPFARDYLASDAVRLVETL
                     RKEAGGAGQGKSAEQLRRDTSAAAARGDYSLAARLAAAAVTANPKDAANWLALARLAI
                     AADDAQWSNRWSLRDEGATAAYGAYERLATPPLQAEALALLGNLMARREMWRPALDAY
                     RASLDRRDDADTQAIYEDMREKHGFRITDYKVDNDSAAPRVCFQFSDPLARKTDFSPY
                     VAVSGATTGAISTEEQQLCVEGLKHGERYAIVIRQGLPSTVGESLLKDADYEIYVRDR
                     SPQVHFSGKSYVLPRDGQQGAPLVTVNTAKVAIDVYRIGDRNLLAATQRDDFLKEIPS
                     YRAKEIADSDGKKVWSGSMDVASELNKEVVTDFPIFQAVGKLEPGLYIVTARPWRGAS
                     DAASESDDSGGNLATQWLVVSDLGLATFSGEDGLHAVVRSLATAAPLAGVEVKLVARN
                     NEVLATKATNAEGRADFDPGLTRGAGGSAPSLIVASTAGGDYNFLSLLQGAFDLTDRG
                     VAGRDAPKALDAFVFTERGVYRSGETVFITALLRDAKGVAAAGLPLTLVAQRPDGVEY
                     KRVVLADQGQGGRAYALPLLPGAAAGAWRIEVYADPKGPSIGETSFLLEDYVPERLDV
                     QLKPAKPILAPGEPAEIGVDAKFLYGAPAAGLDVTGSVTLQAVDGAALAGYPGYAAGL
                     ADDEFDAVTNQLDAKVQTDAKGHADVSIDLPEGTAAKPLEAKIVLRVAEPGGRAVERV
                     VTLPIRAKSTLIGVKKDFDEALGEGQPALFDAIAVAPDGSRVARKGVEWSLYKIDNDY
                     QWYNSEGHWSYEPVKSSRRIAQGTIDIGADAPARISANVSWGRHRLDIKSPDGDQTSI
                     TFDVGWGGTVSADTPDNAVVTLDKASYSAGEEAKLRIASHYAGKASVLVVGDKLDRLI
                     ETDLKEGDNVVPFKVEADWGPGAYAVAITHRPLDVAAKRMPGRAVGLAWFSIDAAQRK
                     LNLSIDAPEKTRPREKLDLAVKIDGLAPGEEAEATVAAVDLGILNLTHFKTPDPADFF
                     FGQRKLPVDIRDLYGLLIDGMQGAAGAIRAGGDGGAALEGNLPTQEPVALFSGVVRVG
                     ADGTAHASFDMPAFNGSVRVMVAAWSKAKVGSAEATVIVRDPVVATGTLPRFLAFGDH
                     SQLHVDLDNVEGDAGDYRLDLDIHGPITAPADALTKTIQLAAHQRAAINVPLTAVGVG
                     TASIDLDLVGPKLEAKQHFVLGVESGAPELYRRTIRMLAGGASQTISSDLLADFVPGT
                     GAVSVAVSPFGALDAPAVLQALDRYPYGCSEQLVSRALPLLYYNRLASIENLGIDPDV
                     DKRIKDTIDRVMTRQDSNGAFGLWQAGAADDDMWLDAFVSDFLTRARERGFDVPKQGF
                     DQALERLRNEVANTADPSAESGQPLAYALYVLARNGRPVIGDLRYLADTKLEAFKTPL
                     ATAQIAAALAMLGDRTRAGKVFAASLQSLRDERDDGYSRPDYGSKLRDGAGALALLAE
                     ANLKGGEVADDALVKVSEVVEQARAERTYTSTQENTWMVLAAEALADRAPAHQFVVDG
                     QTKTGSVYRKWKGFALDAKPSTIVNSSQSPAQIVITTSGVPIAPEPAASSGYDVERSF
                     YKLDGGKFDASRVTQNDRFVVVLKVTEAEAKYARLLVVDRLPAGLEIDNPNLVDGGDV
                     EAFAWLKKDADPAHTEYRDDRFVAALDRNTDQSAFFNLAYVVRAVAPGKYVYPPATAE
                     DMYRPERFGRTAFSTIEVVAK"
     gene            13365..>14428
                     /locus_tag="VKU03_15175"
     CDS             13365..>14428
                     /locus_tag="VKU03_15175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020093719.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HLJ72660.1"
                     /translation="MRNERRWFAALGLAIFFAGLALPGAWFSYVQSLGPIDLASQRDG
                     STIVVDRDGRLLRAFTMPDGRWRLPVRSGDVDPRYLAMLLAYEDHRFYDHGGVDWRAM
                     ARALFQMATRGRIVSGGSTLTMQVARLIEPRQQRSLAAKLRQVARALDIESLVSKQGI
                     IDRYLALAPFGGNIEGVRAASLAYFGKEPRRLSIAEAALLVALPQSPEARRPDRSPDA
                     ARAARNRVIDRAVERGVISKAEGEAAKSEPVPREKKPFPMLAAHAAEEAAAADPRAHV
                     LRLSIDARLQEKLEMLARDSAERLGPKISVAIVAVDNKSGEIRARVGAADYMSIERGG
                     AIDMTRAPRSPGSALKPLIYA"
BASE COUNT         2442 a         4839 c         4868 g         2279 t
ORIGIN      
        1 gttctagcag ggaacgcggc gcggcccaat cgcaacggca agacagcgac tacaagaagt
       61 atccaggttc tagcagggaa cgcggcgcgg cccaatcgca acctgcgcga caaggagggc
      121 gagaattgaa gcgttctagc agggaacgcg gcgcggccca atcgcaaccg gaagcccatc
      181 gcgcgaggcg taaaaacgcc tcgcgcgatg ggctatggct gtcgccatgg gctttcaaga
      241 aatttgccac tcgcgctttg atgcttatag gtaatcgctg caagcatcgg tcgccggccg
      301 gccgcgcttc gctacgcccc gacggccaag gccagcttgt gatgctcgcg cggcgagcgc
      361 ggcagcgcga cgagcacgac cgcgccgtcg ccgggccgcg aattgatccg cagcgagccg
      421 ccatgcaact cgaccagcga ggtcgcgatg gcgaggccga gtcctgatcc tttcatgccg
      481 ttggccatgg tgacctcggc ttgttcgaac ggccgcccga ggcgcgagag atcggccgcc
      541 ggaatgcccg gcccggtatc ctcgacatag atatagatct gctcgtcgaa ggcttgcgcg
      601 ccgatcgaaa tctcgccgcc gtccggcgtg aacttcaccg cgttgcgcag cagcgtcacc
      661 agaacgcgct cgatcgcctt gggatcggca taaacgatct cggtcggatt gacgtcgacc
      721 tcgatcgaca gctttttgtc ccgcgccgcc tcggcgacct ggtggaccga attgcggatc
      781 gccgtctcca ccgcgaggtt ggagcggttg agccggacgc ggcccgcttc gagccgcgac
      841 atgtcgagca cgtcggaaat cacattgtgc agatagcggc cgctgtcgcg gatatgcgaa
      901 cagtaatcgt ggtatttcgg cgagccgagc gggccgaacg cttcctgcga catcatttcc
      961 gagaagccga taatggcgtt gagcggcgtt ctcagctcgt ggctcatatt ggcgaggaat
     1021 tcggccttgg cgcggttggc gctctcggcc tgggccttct gctcgagata gcgctcggca
     1081 agatcggcga gctgctgcgt ctgcgcctcg agcgcctggc gcgagcggcg caattcggcg
     1141 acgctggcga gaaggcggcg ctcggaatcc atcaattgct cttcatggcg cttcagcgcc
     1201 gtgatgtcgg tgccgaccga gacatagccg ccgtccttgg tgcggcgctc gttgatctgc
     1261 agccatcgtc cgtcggtgag ccgcgcctca taggcgcctg gcttgccggc ttcgccgctt
     1321 tcgctggcgc gcagcgcgac ctcgttgcgg atcatcggcg gcgcgccttt ggcggcgagt
     1381 tcggcgtaag ggacgccggg ccgcaccgct tccggcggca gattgtagag gcgttggaat
     1441 ttcgaattgc agaggacaag atggtttgaa gaatcccaca acacgaaggc ttcggagatg
     1501 gcctcgatgg cctcgcgcag cctctggtcg gcggtggcgc tgatctcggc cagcgccttc
     1561 tgctcggtca cgtcgagcgc gacgccgacg agatggcggc cgctcgaaga atcgctgacc
     1621 agttcggcgc gggcgcgcag ccagacccag tcgccggcgg cgttgcgcag gcggaattca
     1681 tgatcgacgg tcggctgctc gccgtcgatc acgccttgcg cgatcgccga cagatcgccg
     1741 tcctcgggat ggaccatcgc ctgtacatcg ccgatcgaga ggagatcgag tgatggcggc
     1801 aaatcgagca actcgtacat cgagcgcgac cattgaatcc tgccgcgcga caaatcccag
     1861 tcccagaggc cgcagcggcc gcgattgagc gccgaatcga cccggttgcg cgcgctgcgc
     1921 gccaacgtct cgatccgccg cgcccgcgcc gcttgccgat aaggaacggc gaagcacgcg
     1981 acaagaagca gcgccgccgc agtaagcagc agagcggcgc ggatggcgga ggcgcgccat
     2041 gaggcgagca ggccttcggc gcgcatgata taagcgagct gcgccagcgg cggccgcaac
     2101 gatcgtaccg ttgcaaaggc gtcagccccg tcggcggtga cgatccgcaa cacgccggct
     2161 ttctcgccga aaatcgccag cgggcccggt ccgagcacgg cggcgagatt cgccccggcc
     2221 gccggcggct cgctcgccga aaggccgccg tcagcgccgg cgatcagcgc atcgccttcg
     2281 cgcaaggctt cgtcgccgcc aagcgcgacg cgaagcaggt ccgccggcgc gccggcggga
     2341 ttggcggcga gcgcctcgtt caggcggaaa gcgagcaaag aggcgcgcag gcccacttca
     2401 tgctcggcgg cgcgaagcgc gcgctcgcgt tcgaggcgca tctgcgcggc gatcgtcagg
     2461 gtcgcggcgg cgaagatcag cgccgccaag gccggcgcaa catagagaag ccagagccgg
     2521 acctggagcg gcgagcggaa gcgaagcgag tgcgcggtcg ctgcagcgaa attccgccag
     2581 ccagccgcgc gcatatcccc attggcggcg tttgcacgcg ccatcgtggc cccctcgtcg
     2641 tcgctacgac ttatgattcg gcgaaatgtg ccgcatcacc ccgaatcagt ctcataagaa
     2701 tcccgatgcg aatcgttgtc cagagattaa tggaaggttg atgaaatttt taaactccgg
     2761 cgccgcaagg ccgcgcgccg cggttgcgcg ggcgccgcac aagcgcgctg tgcgagtcgc
     2821 cgccggtccc atcggcttgt ttcgagaggg tacgtgacgc tcgaaaagag ccggccgtta
     2881 acgattgtgg ccgaccaacc caaggccagg tacttgaggg gcggcgcgcc ggctcagctc
     2941 aagctgcggg tgacgatgtc gctcaaatcc gccgagagcc cggcttgcgc cgcgatgcgc
     3001 cgcagcgtcg cctcggcccg ctcctggcgc ttgggctcga aggagcgcca cgagcgcaag
     3061 gccgagagga gccgcgcggc gatttgcgga ttgctgggat cgagcttcag cacgacttcg
     3121 gccaagagct cgtagccgag cccgtcggcg cggttgaact gggtctgatt ggctgcgaag
     3181 ccgccgatca gcgcgcgcac ccggttggga ttgctcaggg agaaagaatg atgggccatc
     3241 agcccgcgca cgcggtcgag cgtctcgcgc tcggggatct gcgcttgcag ggcgaaccac
     3301 ttgtcgagga tcaatgcgtc atcggcatgg gcgcgccaga aggattcgag cgcatgctcg
     3361 cgcgcttcgc ccgggatggc ggcgagggcg gcgagcgcgg caaagcgctc ggtcatattg
     3421 ttggcctcgg cgaattggcg ctgcgcccgc tcgacgcagc ccggttcgcg tcccgccgtc
     3481 agcatggcga gcgccagatt gcgcaaggcg cgccggccgg cgctgcgggc gtccgggctg
     3541 aacggcccgg cgtcgaagag cttgtcgtag atttgcgcta aggcggcggc gtgggcgcgg
     3601 cctaggctcg cccgcaaaaa ctcgcgggcg gcgaaaatcg cgtcaggatc gacgtttttc
     3661 gccgtttcgc gggcgagatc ggcttcgctc ggcaaggtcg cggtcaaggc ggcgaaagcg
     3721 aaatcgagct tgccgtcgag cgcgtcggcg agcacgccgc cataggcggc ggaaaagcct
     3781 gtctcgatct cggccggacg accggcgcgg atcgcttcga cggcccgaag caggacgcgg
     3841 gtcgcaaggc tctgcaccgc ctgccagcga ttgaaactgt cgcgatcgtg tcgcgacagg
     3901 atcaagagat cgctctcggt cagatcgtcg tcgacccgca ccggcgcgga gaagccgcgc
     3961 agcagggaga gcgccggccg cgccggcacg ttcttgaaga cgatccggcg cgacgccgca
     4021 ttgagctcga aaacgccgcg cgccgtctcg ctggccgtcg cgtcgccgct cgaaagagcg
     4081 atctcctcgc cggtcggcgc gaccaagccg agcgcgatcg gaatcaccac cggcgccttc
     4141 accgcttgat cgcgggtcgg cggcgtcgcc tgggtgaggt cgagcgtgta ggttcgcgcc
     4201 gccgcgtcat aggcgccgcg caccgtcaga agcggcgtgc cggcctggct gtaccagcgc
     4261 ttgaattcgc gcagatcgcg tcccgagacg tcagcgaagc attgcaggaa atcttcgacc
     4321 gtcgccgccg tcccgtcgca acgctcgaag taaaggtcca tgccgcggcg gaacgcgtcg
     4381 gcgccgatca gcagtttcag catgcggatg acttcggcgc ccttctggta caccgtcgcc
     4441 gtgtagaaat tgttgatctc gtgatagact tccggccgca cattatgcgc gagcggcccg
     4501 gcgtcctccg gaaactgcgc ggcgcgcagg ccgcgtacgt cggcgatccg cttgaccggg
     4561 gccgagcgct ggtcggcgga aaaatcctgg tcgcggaaaa ccgtcaggcc ctccttgagg
     4621 catagctgga accagtcgcg gcacgtgatc cgattgcccg tccagttgtg gaaatattca
     4681 tgggcgatca cgccctcgac gccggcgtaa tcgtcgtcgg tcgcagtctc ggggagcgcc
     4741 aggacgtatt tgtcgttgaa gacgttgagg cccttgttct ccatcgcgcc catattgaaa
     4801 tccgagacgg cgacgatgtt gaagacgctc aaatcgtatt cgcggccata gactcgctcg
     4861 tcccacgcca tggcgcgctt cagggaatcc atcgcgtaat aggcgcggtc ttccttgccg
     4921 tgctcgacat agatggcgag cttgacctcg cgcccggatc gcgtgacgaa gcgatcttcg
     4981 atatgggcga gatcgcccgc aaccagggcg aagaggtagc agggtttctt gtacggatcg
     5041 cgccacacgg cgtaatgccg tccgtccgcc gcttcgcccg tctcttcgag atcgccattg
     5101 gcgagcagga ttggcgcttc cttcttgtcg gcttcgagcc gggtcacata ggtcgaaaga
     5161 acgtccggcc ggtcgaggaa atagctgatg cgcctgaacc cctcggcctc gcattgcgtg
     5221 caataggccg agccggagcg atagaggccc atcagcttgg tgttggcgga aggatcgagc
     5281 tcggtttcga tccgcagcgt gaacgggcgg cgcggcggat tgggcagggt gagcttgtcc
     5341 ggcgtcgcct cgaaagcgcc agcgtcgagc ggcgcgccgt ccagcgagac cgaaagcagg
     5401 cgcaattcgt cgccgtccag atcgagcgcg acattcgccc ggccggccgg atttggccgc
     5461 aacttcaacg tcgaaacgac gcgggcggcg cgcctgtcga gcgcgatgtc gaggcgcacg
     5521 tcgtcgatca gatagtccgg cggtcgatag tcggaaaggc ggatcggctg accggttgtg
     5581 gtgcgcaaga aagcgtcctc ttgagagatc caactcaggg cgcggcccgc cgttccccgg
     5641 cgttggatga atcgccgttc aggcgaggcg cgtcaagccg cgcgctgaag ccgcggtcgg
     5701 cgaccgcggc ttcagcgccg ccgtcatttc agggagcgtt cgtacttcca gcgaaaaccg
     5761 tttcagcgcc tcggcgagcg ccgggctcgg cggcgcgccg ctggtgaaaa tcgccggcgc
     5821 gaggcgatcc gcgcgcttcg ccaaagcctc tgccggcccg agcaggtggt cgacgcggcg
     5881 ggcgatggcg ggcgcaggat cgatgtaatc gaccggccaa ggggcaagcg cggcgaggcg
     5941 ctcggctagc aaaggataat gcgtgcaagc cagaaccacc acatcggtgc gccggccctt
     6001 ccgcgccacg aaacacggcg cgatctcctt ggcgatggcc tcgtcggcga cggccaggcc
     6061 cttgatatgc gcctcggcga gccgggcaag gtcgggcgcg ccgaccagct tgacctcgca
     6121 gccggcggcg tattcgcgcg ccagcgcctg ggtatagtcg cgcgcgaccg ttcccggcgt
     6181 gccaaggaca ctgatcaggc gcgagcgggt tatcgccgcc gccggcttga tcgccggcac
     6241 ggttccgaca aagggaagcg cgggatggcg cgcgcgcagc ggcccaagcg ccagggtcga
     6301 ggcggtgttg caggcgatga cgacgagatc cggcgcgagc gcggcgatca actcgccgac
     6361 gatcgcgacc acccgttcga ccaaagcttg ctcggcaagg gcgccatagg gaaaagcggc
     6421 gtcgtcggcc gcatagaaaa gctcgtcgga ggcgcgcgaa gcggcgatct gctcgtagac
     6481 ggtcaggccg ccgagaccgg aatcgaaaac gagaatccgc ataaggcgcg ccgctttgtc
     6541 agtcgcagac gatcgggacg acgagagcgc ctttttcggc ggcgatccgg ccggcggcgc
     6601 ttgcgccagc ctccgtcgcc ttggccaggg cgatatcggc gccggccaga aaccgctcga
     6661 tgtcggacgc ggcgacgacc ggatgcggcg cgagcgggat cacgccggcg accggcttgg
     6721 cttcggccgc cgccggcgcg acgatcgcga ccaaggcgcc gctgcggtcg aagaccgggc
     6781 tgcccgcggc gttctgcgga agcgaggcga ggagacgcgg cttggccgcg tccgtcgcgc
     6841 cggcaagcgg cgcaatcgcc gcgacctgca aggcgggctt ggcgccggcc ggatcggccg
     6901 cgtaggtcag cgcgacgaga tcgtcgctca ggggcgcgac ggcgagcggg gcggctgcgg
     6961 gcttgccggc gaagcccgcg ccgatcaggc tgaggcccgc ctgcttgtcc tcgcgcagat
     7021 atttcgccgg cttgccgtcg acgctgggat gcggacaggc gccggaatcg agcgccgtca
     7081 gcgcctcgcc cgccgccacg atcaagccgt ttgccgcgag cgccggcttg gacggcgtcg
     7141 gcgccttggc cggaaccgtc gtcgcattcg ccgcgggcgt cgcggccgcc gggaaaggct
     7201 caaacgtatc ggcgatggcg acggcgatgc ggtcgaagct tgcggattgg gcgggatagg
     7261 tcagcttgaa cccgcgcact tggctcggat caggccaatt ggtcggcgct ttggcgtagc
     7321 gcagatagaa tttgcggccg ccctcctcgc cggaaaggac gaaaaagtcg ggccgcgaca
     7381 atttcaatgt cgtcttgcgg ccgggcgtgt cggcgctgag gcgcgcatag agcgccgcga
     7441 gatcgccgcc ggcgctgacg aggtcgagcg tcgcactgcc ttccgcattg gcaagccgcg
     7501 agccgttcgg cagcgcggcg cgccgatcga ggagcctcag cggcgcgccg atcctgacgc
     7561 ctgatttgtc gtcggcgacg atctggaatt tcaccgccgc cttcgctttg ccggcgacgg
     7621 cgatcagccg ggccagagcc gccggatcga gaataccggt ggtcggcgtt ttggcgtcgg
     7681 cttgataggc gaggatcgca tcgcgcgtac gcttgccgaa attgccgtcg acgacgccgt
     7741 tataatcgcc ggtccaaatg agcgcgtcct gaatggcgcg gcgatcggct tcggacaggg
     7801 cctcataggc ggctttttgc gcttgaaaga cctgatcggc cgcgccagcc gccggcggcg
     7861 aggcgttctg cgccgcgaag gcggcgtcgc cgccgatcaa gaaagcggcc gcgccgatca
     7921 gaaaggcaag ccgcggaccc ggccgcataa aaatcgccat gaccccctcc atgcgccccc
     7981 gtcctcgtag catcgcggcg gcggcgcgtc acatgcgacc gccggtttat aaacgccgcc
     8041 gaaccagagg acccgacttt acggtctgcc cgcgcagacc gtaatgttat tgccgacgct
     8101 ccggcctacc agcgagaccg attcatgcgc ctctggctct cctcccttct tatcattgtt
     8161 gtcggcgctt tcgccgccgg ggcgcaagcc gccgagatca agcccttcgc ccgcgactat
     8221 ttggcgagcg acgccgtccg gctggtcgag accctgcgga aggaggccgg cggcgcggga
     8281 cagggcaaat cggccgagca attgcgccgc gacaccagcg cggccgccgc ccgcggcgat
     8341 tacagcctgg cggcgcggct ggccgcggcg gcagtcaccg ccaatcccaa agacgccgcg
     8401 aattggctgg ccttggcccg gctcgccatc gccgccgacg acgcgcaatg gagcaaccgc
     8461 tggtctctgc gcgacgaagg cgcgaccgcc gcctatggcg cctatgagcg cctcgccacg
     8521 ccgccgctgc aagccgaggc gctcgctttg ctcggcaatt tgatggcgcg gcgcgaaatg
     8581 tggcgcccgg ctttggacgc ctatcgcgcc agcctcgacc ggcgcgacga cgcagacacc
     8641 caggcgatct atgaagacat gcgcgaaaag catggcttcc gcattaccga ctacaaagtc
     8701 gacaacgatt cggcggcgcc gcgcgtctgc ttccagtttt ccgacccgct cgcccgcaag
     8761 accgattttt cgccctatgt cgccgtctcc ggcgcgacga ccggcgcgat cagcaccgaa
     8821 gagcagcagc tctgcgtcga aggtttgaag catggcgagc gctacgccat cgtcattcgc
     8881 cagggcctgc cctccactgt cggcgaatcc ctgctgaagg acgccgatta cgaaatctac
     8941 gttcgcgacc gctcgccgca ggttcatttc tccggcaaga gctacgtcct gccgcgcgac
     9001 ggccagcaag gcgcgccgct ggtcacggtc aacaccgcca aagtggcgat cgacgtctat
     9061 cggatcggcg accgcaacct gctcgccgcg acccaacgcg acgatttctt gaaggagatc
     9121 cccagctatc gcgccaagga gatcgccgac agcgacggca agaaagtctg gagcggctcg
     9181 atggacgtcg cctccgagct caacaaggag gtcgtcaccg attttccgat tttccaggcg
     9241 gtcggcaagc tcgagcccgg gctctatatc gtcaccgcgc ggccctggag gggcgcgagc
     9301 gacgcggcga gcgagtccga cgacagcggc ggcaacctcg cgacccaatg gctggtcgtc
     9361 tccgacctcg gcctcgcgac gttttccggc gaggacgggc tgcacgccgt ggtccgctcg
     9421 ctggcgacgg ccgcgccgct cgccggcgtc gaggtcaagc tcgtcgcccg caacaacgaa
     9481 gtgctggcca cgaaggccac caacgccgaa ggccgcgccg atttcgatcc gggcctgacg
     9541 cgcggcgccg gcggctcggc gcccagcctg atcgtcgcct cgaccgccgg cggcgactac
     9601 aatttcctca gccttttgca gggcgcgttc gatctcaccg accgcggcgt cgccggccgc
     9661 gacgcgccga aagcgctgga cgcctttgtc ttcaccgagc gcggcgtcta tcgctcgggc
     9721 gagacggtgt tcatcacggc tctgctgcgc gacgccaagg gcgtcgccgc cgccggcttg
     9781 ccgttgactt tagttgcgca gcggccggac ggcgtcgaat acaagcgcgt cgtcttggcc
     9841 gatcaaggcc agggcggacg cgcttacgcg ctgccgcttc tgcccggcgc ggcggcgggc
     9901 gcttggcgga tcgaggttta cgccgacccc aaggggccga gcatcggcga gacgagtttc
     9961 ctgctcgaag actacgtacc cgagcgcctc gacgtgcaat tgaagccggc gaaaccgatc
    10021 ctcgcgccag gcgagccggc cgaaatcggc gtcgacgcga aattcctcta tggcgcgccc
    10081 gccgcaggtc tcgacgtgac cggctcggtg acgctgcagg cggtcgacgg cgccgcgctc
    10141 gccggctatc ccggctatgc ggccgggctc gccgacgacg aattcgacgc cgtcaccaat
    10201 cagctcgacg ccaaggtgca gaccgacgcc aagggccatg ccgacgtttc gatcgacctt
    10261 cccgagggga ccgccgccaa accgctggag gccaagatcg tgctgcgcgt cgccgagccg
    10321 ggcgggcgcg ccgtcgaacg ggtcgtcacg ctgccgatcc gggcgaaatc gacgctgatc
    10381 ggcgtgaaga aggatttcga cgaagcgctc ggcgaaggtc agcccgcctt gttcgacgcc
    10441 atcgccgtcg cgcccgacgg aagccgcgtc gcccgcaaag gcgtcgaatg gtcgctctat
    10501 aagatcgaca acgactatca atggtacaac agcgaggggc actggagcta cgagccggtc
    10561 aagtcgtcgc ggcgcatcgc ccaagggacg atcgatatcg gcgccgacgc gccggcccgg
    10621 atcagcgcca atgtgagctg gggccgccat cggctcgaca tcaaatcgcc cgacggcgac
    10681 cagaccagca tcacgttcga tgtcggttgg ggcggaacgg tcagcgccga cacgccggac
    10741 aacgccgtcg tgaccttgga caaggcgagc tacagcgccg gcgaagaggc caagctgcgc
    10801 atcgcctccc attacgccgg caaggccagc gtgctggtcg tcggcgacaa gctcgaccgg
    10861 ctgatcgaga ccgacctgaa agaaggcgac aatgtcgtac ctttcaaggt cgaagccgat
    10921 tggggtcccg gcgcctacgc cgtggcgatc acccatcggc ctttggacgt cgcggccaag
    10981 cggatgcccg gccgcgccgt cggcctcgcc tggttctcga tcgacgcggc gcagcgcaag
    11041 ctcaatctgt cgatcgacgc gccggaaaag acgcggccgc gcgagaaact cgatctcgcc
    11101 gtcaaaatcg acggcctcgc gcccggcgag gaagccgagg cgacggtcgc cgcggtcgat
    11161 ctcggcatcc tcaacctcac ccatttcaag acgcccgacc cggccgactt cttcttcggc
    11221 cagcgcaagc ttccggtcga catccgtgac ctttacggcc ttctgatcga cggcatgcag
    11281 ggcgcggccg gcgcgatccg cgcaggcggc gacggcggcg cggcgctcga aggcaatctg
    11341 ccgacccagg agccggtggc gctgttctcc ggcgtcgtgc gggtcggcgc cgacggaacc
    11401 gcccatgcct ccttcgacat gccggccttt aacggctcgg tccgggtgat ggtcgcggcc
    11461 tggtcgaagg caaaagttgg cagcgccgag gcgacggtaa tcgtccgcga tccggtcgtc
    11521 gcaaccggga ccttgccgcg cttcctggcg ttcggcgatc attctcagct tcatgtcgat
    11581 ctcgacaatg tcgagggcga tgccggcgat tatcggctcg atctcgacat tcatggcccg
    11641 atcacggcgc cggccgacgc tctgacgaag acgatccagc tcgccgccca ccagcgcgcc
    11701 gcgatcaacg tgccgctgac ggcggtcggc gtcgggacgg cgagcatcga tctcgatctg
    11761 gtcgggccga agctcgaagc caagcaacat ttcgtgctcg gcgtcgaaag cggcgcgccg
    11821 gaactctatc gccggacgat ccgcatgctg gccggcggcg caagccagac gatttcaagc
    11881 gatcttctcg ccgatttcgt gccgggcaca ggcgcggtct cggtcgcggt ttcgcctttc
    11941 ggcgcgctcg acgcgccggc cgtgctgcaa gcgctcgacc gctatcccta cggctgctcc
    12001 gagcaactgg tcagccgggc gttgcccctc ctctactaca accgcctcgc ctcgatcgag
    12061 aatctcggca tcgatcccga tgtcgacaag aggatcaagg acacgatcga tcgcgtcatg
    12121 acgcgccagg attcgaacgg cgccttcggc ctctggcagg ccggcgctgc ggacgacgat
    12181 atgtggctcg atgctttcgt cagcgacttt ctgacccgcg cgcgcgaacg cggcttcgac
    12241 gtgcccaagc agggcttcga tcaggcgctg gagcgcttgc gcaacgaggt cgccaacacg
    12301 gccgatccga gcgctgaatc cggacagccg ctcgcctacg ccctgtacgt cctggcgcgc
    12361 aacggccgcc cggtgatcgg cgatttgcgc tatctcgccg acaccaagct cgaagccttc
    12421 aagaccccgc tcgccacggc gcaaatcgcc gccgctctcg ccatgctcgg cgaccgcacg
    12481 cgggccggca aagtcttcgc ggcctcgctg caatccctgc gcgacgagcg cgacgacggc
    12541 tattcgcggc ccgattacgg ctcgaagctg cgcgacggcg ccggcgcgct cgctttgctc
    12601 gccgaagcca atttgaaggg cggcgaggtc gccgacgacg cgctcgtcaa agtttcggaa
    12661 gtcgtcgaac aggcgcgcgc cgagcgcacc tatacgagca cgcaagagaa cacctggatg
    12721 gtgctcgccg ccgaggcgct ggccgaccgc gctccagccc atcaattcgt cgtcgatggg
    12781 cagacgaaaa ccggttcggt ctatcgcaaa tggaaaggct tcgcgctcga cgccaagcct
    12841 tcgacgatcg tcaattcgag ccagagcccg gcccagatcg tcatcaccac gtccggcgtg
    12901 ccgatcgcgc cagagccggc cgcctcgtcg ggctacgatg tcgagcggag cttctacaag
    12961 cttgacggcg gcaagttcga cgccagccgg gtgacgcaga acgaccgctt cgtcgtcgtc
    13021 ctcaaagtga cggaggccga ggcgaaatat gcaagactgc tcgtcgtcga ccgcttgccg
    13081 gcgggcttgg agatcgacaa tcccaatctc gtcgacggcg gcgatgtcga agccttcgct
    13141 tggctgaaaa aggacgccga tccggcccat accgagtacc gcgacgatcg tttcgtcgcg
    13201 gcgcttgatc gcaataccga tcaatcggcc ttcttcaacc tcgcctatgt cgtgcgcgcg
    13261 gtcgcgccgg gcaaatatgt ctatccgccg gcgacggccg aggacatgta ccggcccgag
    13321 cgcttcggcc gcaccgcgtt ttcgacgatc gaggtcgtcg ccaaatgagg aacgagcggc
    13381 gctggtttgc ggcgctcggc ctggcgatct tcttcgccgg gctcgccctg cccggcgcct
    13441 ggttcagcta tgtccagagt ctcggcccga tcgatcttgc ctcgcagcgc gacggctcga
    13501 cgatcgtggt cgatcgcgac ggaaggctgc tccgcgcttt caccatgccg gacggtcgct
    13561 ggcggctgcc ggttcgcagc ggcgatgtcg atccgcgcta tctcgccatg ctgctcgcct
    13621 atgaggatca tcgcttttac gaccatggcg gcgtcgactg gcgggcgatg gcgcgcgcgc
    13681 tgttccaaat ggcgacgcgc gggcgcatcg tttccggcgg ctcgaccctg accatgcagg
    13741 tcgcccggct gatcgagccg cgccagcagc gctcgctcgc cgccaagctg cgccaggttg
    13801 cgcgcgcgct cgacatcgag agcctcgtct ccaagcaagg aatcatcgac cgctatctcg
    13861 ctttggcgcc gttcggcggc aatatcgagg gcgtgcgcgc cgcctcgctc gcttatttcg
    13921 gcaaggagcc gcgccgcctc tctatcgcgg aagccgcttt gctcgtcgcc ttgccgcaat
    13981 cgccggaagc ccgccgcccc gaccgttcgc cggacgcggc gcgcgcggcg cgcaatcggg
    14041 tgatcgatcg ggctgtcgag cgcggcgtca tttccaaggc cgagggcgag gccgccaaaa
    14101 gcgagcccgt tccgcgcgag aagaagccgt tcccgatgct cgccgcgcat gccgccgaag
    14161 aggcggccgc cgccgatccc agggcgcacg tgctgcgcct ttcgatagac gcgcgcttgc
    14221 aggaaaagct tgagatgctg gcccgcgaca gcgccgagcg gctcggcccg aagatttccg
    14281 tcgccatcgt cgcggtcgac aataagagcg gcgaaatccg cgcaagagtc ggcgccgccg
    14341 attacatgtc gatcgagcgc ggcggcgcga tcgacatgac gcgggcgccg cgctcgcccg
    14401 gttcggcgct gaagcccctc atctacgc
//
LOCUS       DATGJW010000504         7382 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_96390, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000504 DATGJW010000000
VERSION     DATGJW010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7382)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7382)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7382
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96390"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..937)
                     /locus_tag="VKU03_15180"
     CDS             complement(<1..937)
                     /locus_tag="VKU03_15180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013850368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ72661.1"
                     /translation="MKRRDFLKLSTAVAGATLTTYLPAGWQAQAAAKWKIGFSQCTTL
                     EPWRVQFNKEIEAEAKKHPEVDLIITDGLDKTEKQVADVENLIVQQVNVLLISPKESA
                     GLTGVVDKAIDAKIPVIVLDRNVDTKRITQFIGGDNKLIGRKVGEYAVKLLGGPGKAQ
                     GNVVEIWGGMGTQPAHDRHDGFHEFTDKEPGIKMLLNNQSGDWKQDQAYNIMTTALRN
                     NEKIDLVYGHNDPMAYGAYLAAKDAGREKDIKFFLGIDGLPDEGVEWVNKGLLTATFR
                     YPTPGAEGLRQAIKLLNGEKIPPVITLPTLLITKEN"
     gene            complement(924..2258)
                     /locus_tag="VKU03_15185"
     CDS             complement(924..2258)
                     /locus_tag="VKU03_15185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72662.1"
                     /translation="MTALRVLSRTKLYWGLLLIFAIGAALSPHTSSGRNIFLSYANLT
                     DVLRQVSITGLVATGMTIVILLGGIDLSVGSVMGFSTVVCAMLLTKPGWTAASAIAVP
                     AVALVSFAAVFFLARFVLGGLARGRDAHVGRRREIDLGPWRGLWAPCLLGLIAAALAF
                     AFVTLQVPTKFGVVAVLLATPCVALLLGSLNGAIIVGGRLQPFIVTLAMMVAALGLGR
                     LTAGQDNAVVPVYTGTNATEAFETLRSMIGGVLPMPGVFFLAAIILFGAILRFTSFGR
                     YIYAIGGNIEAARLSGVNVVQVQFTAYLLSGLLAGVAGVLFVAQYRQGKPDAGTGLEL
                     DAIAAVVIGGTSLMGGRGGLAGTLVGVLIFGLLSDILQLQNIDSNVQLVMKGLIIVCT
                     VLVQEQNLGQLIARLRRIAPPPTREPAGSAGAGRPRELLQTVKVREDFDEAS"
     gene            complement(2255..3871)
                     /locus_tag="VKU03_15190"
     CDS             complement(2255..3871)
                     /locus_tag="VKU03_15190"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006325857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ72663.1"
                     /translation="MRLWRGASQISEEPLARAPEASAERGAIVLAAKGVSKAFSGVWA
                     LQGVDFDLKCGEVHALMGENGAGKSTLMKILAGVHGDYLGAISVAGQDVTFNGVRDAE
                     LAGIAMIHQELNLVPELSVAENIFLGREPLIAGLVIDRRRIVAVARALLRRLNIKIDP
                     EARVGSLRVGEQQLIEIAKALSLDTRILIMDEPTSALSASECETLFKVVRQLAASGVA
                     IIYTSHRLDEVIALADRVTVLRDGRRVLTSPIDKISLDGIISAMVGRDVTTSGRNEPA
                     GSAAPILSVENLTFDVATPRGWRRVLHGVSFDLRRGEILGIGGLLGSGRSEIVEAIFG
                     AGRGWRGGTITIDGAPATINSPADAYRLGVALVTEDRKTRGLHLGSTIRDNIALPSIG
                     AMSRFGLRAFAREAALAADVVGRLSVRCTGIEQVASTLSGGNQQKIVIGKWLATEPRV
                     LLLDEPTRGIDVGAKQEIYQLIFKLAAEGLAIVVVSSEMPELLLLSDRILVMCEGRQT
                     GILRRAEATQEAVMRLAAPGVAGDFRKMPA"
     gene            4034..4735
                     /locus_tag="VKU03_15195"
     CDS             4034..4735
                     /locus_tag="VKU03_15195"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HLJ72664.1"
                     /translation="MGTAMPSATFQSEAGRQSKADRVYLSLKQAIVSGELPPNALIDK
                     LELSARFQVSRLSVTAAVNRLAFEKLVFVEPQRGSYVAKIRLSDARQWMLVRRALEME
                     VVATCARELPDAAIERLGRNVAYQRTALDAGDMQGFHELDTVLHLQLVDALGWGRVAE
                     ILDPVRTHLERVRRTLLPEPGRMERTFAEHQEIYRAIAAREPDAAREAMERHLNQVLR
                     ELEIFVTRHPGFFEA"
     gene            complement(4773..5984)
                     /locus_tag="VKU03_15200"
     CDS             complement(4773..5984)
                     /locus_tag="VKU03_15200"
                     /EC_number="4.2.99.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012384038.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="O-succinylhomoserine sulfhydrylase"
                     /protein_id="HLJ72665.1"
                     /translation="MTPRKTRDHKISREATKLVHGGSLRSGFQETSEALFLTQSYLYD
                     TMEEAEARFKGDDPGFIYSRYSNPTVAMFEKRMALLEGAEAARATASGMAAITAALMG
                     QVKAGDHIVAARALFGACLYVVEELLPRFGVQSTLVDGTDLQQWRAAMRDNTKTLFLE
                     SPTNPTLEIIDIAAVAKIAREHGATLVVDNVFATPLMQHPLQLGADCVVYSATKHIDG
                     QGRTLGGVILASEAFIAAHVHTFLRQTGPSLSPFNAWVMLKSLETLAIRVKAQTRSAE
                     KIADFLSGHPHIERVLYPGRPDHPQHALAKRQMKGGGTLVTFEVKGGKPAAFKVANAL
                     SIIGISNNLGDAKSLVTHPATTTHQRLKPDARARLGIGDGMIRLSVGLEDVEDLIEDL
                     DQALGALGTKA"
     regulatory      complement(6037..6115)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00521"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00521"
     gene            6338..>7382
                     /locus_tag="VKU03_15205"
     CDS             6338..>7382
                     /locus_tag="VKU03_15205"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020173494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2'-deoxycytidine 5'-triphosphate deaminase"
                     /protein_id="HLJ72666.1"
                     /translation="MVGRKMIVSSQTIEESQFQPASLDLRLGKEAYRMRASFLPGRNR
                     SVLDHLESLKPERISLTGDGAVLEKGIVYIAALQERLDLLPSLSAAANPKSSTGRLDI
                     FTRLIVDRGELFDEAPLGYSGPLYAEISPRSFSVRVREGSKLNQIRFRSRNSQQKSLS
                     DFALPDKDLRSRHAASPLVDRELDLREGLILRVGLEGSPGQVVGYRAIKNSEVIDVDR
                     PGAYPAAEFWEPILARRDKRLILDPDEFYILASRERVQIPPDLAAEMAPIDPAIGEFR
                     VHYAGFFDPGFGHAADGRPSARAVLEVRSRDVPFLLEDGQPVGRLVFEKLADRTDALY
                     GSTAVSNYQGQGLK"
BASE COUNT         1286 a         2418 c         2416 g         1262 t
ORIGIN      
        1 cgttctcctt ggtgatcagc aaggtcggca gggtgatgac cggcgggatt ttctcgccgt
       61 tgagcagctt gatcgcctgg cgcaggcctt cggcgccggg cgtcggatag cggaacgtcg
      121 cggtcagcag gcccttgttc acccattcga cgccctcgtc gggcaggccg tcgatgccga
      181 ggaagaactt gatatccttc tcgcggcccg catccttcgc ggcaaggtac gcgccatacg
      241 ccatcggatc gttgtggccg tagaccagat cgatcttctc gttgttgcgc agcgccgtgg
      301 tcatgatgtt gtaggcctga tcctgcttcc agtcgcccga ctggttgttg agcagcatct
      361 tgatgcccgg ctccttgtcg gtgaactcgt ggaagccgtc gtgccggtca tgcgccggct
      421 gcgtgcccat gccgccccag atttcgacga cattgccctg ggccttgccc gggccgccga
      481 gcagcttgac cgcgtattcg ccgaccttac ggccgatcag cttgttgtcg ccgccgatga
      541 actgggtgat ccgcttcgta tcgacattgc ggtcgagcac gatcaccgga attttggcgt
      601 cgatcgcctt gtcgacgacg ccggtcagcc cggccgattc cttcggcgag atcagcagaa
      661 cgttgacttg ttggacgatc aggttctcga catcggcgac ctgcttttcg gtcttgtcga
      721 gcccgtcggt gatgatgaga tcgacttccg gatgcttctt ggcctcggcc tcgatctcct
      781 tgttgaactg cacgcgccag ggctccagcg tggtgcattg gctgaagccg atcttccatt
      841 tcgccgcggc ttgggcttgc cagccggccg gcaggtatgt ggtcagcgtc gctccggcga
      901 cggcggtcga caatttgaga aaatcacgac gcttcatcga agtcctcccg tactttcaca
      961 gtttgaagaa gttcccgcgg ccggccggcg ccggccgatc ccgccggctc gcgcgtcggc
     1021 ggcggcgcga tgcgcctgag cctggcgatg agctggccga gattctgctc ctgcacgagg
     1081 accgtgcaga cgatgatcag ccctttcatg acgagctgaa cgttcgaatc gatgttctgc
     1141 aattgcagga tgtcggagag gaggccgaaa atcagcacgc cgaccagcgt gccggcgagg
     1201 ccgccgcgcc cgcccatcag gctggtgccg ccgatcacca ccgccgcgat cgcgtcgagt
     1261 tcgaggccgg tccccgcgtc gggcttgcct tggcgatatt gggcgacgaa caacacgccg
     1321 gcgacgccgg cgagaagccc ggacaaaagg tacgccgtga attgcacctg gacgacattg
     1381 acgccggaga gccgcgccgc ctcgatattg ccgccgattg cgtagatata gcggccgaac
     1441 gaggtgaagc gcaggatcgc gccgaacagg atgatcgcgg cgaggaagaa cacgcccggc
     1501 atcggcagga cgccgccgat catcgagcgc aaggtttcga acgcttcggt ggcgttggtg
     1561 ccggtataga ccggcacgac ggcgttatcc tggccggcgg tcaaccgccc gagaccgagc
     1621 gcggcgacca tcatcgccag cgtcacgatg aaaggctgca gccgtccccc gacgatgatc
     1681 gccccgttca gcgagccgag caggagcgcg acgcagggcg tcgccaagag cacggcgacg
     1741 acgccgaact ttgtcggaac ttgcaaggtc acgaaagcaa aagcgagggc cgctgcgatg
     1801 aggccgagga ggcaaggcgc ccagaggccg cgccaaggcc cgagatcgat ttcccgccgc
     1861 cggccgacat gggcgtcgcg cccgcgcgcc aatccgccaa ggacgaaccg ggcgagaaaa
     1921 aacaccgccg cgaagctcac cagggcgacg gccggaaccg caatcgccga ggcggccgtc
     1981 cagccgggct tggtcagcag catcgcgcag accacggtcg aaaaccccat gaccgagccg
     2041 accgaaaggt cgatgccgcc aagcaggatg acgatcgtca tgccggtcgc aacgagcccg
     2101 gtgatcgaga cttgccgcag cacgtcggtg agattggcgt aggagagaaa gatgttccgg
     2161 cccgacgagg tatgcggcga gagcgcggcg ccgatcgcga agatcagcaa aaggccccaa
     2221 taaagcttgg tgcgggagag gacgcgcaga gcggtcatgc gggcattttc ctgaaatcgc
     2281 cggcgacgcc cggcgccgcg agccgcatca cggcttcctg cgtcgcctcg gcgcggcgca
     2341 aaatcccggt ctgccgcccc tcgcacatca ctaaaatccg atccgacagc aagagaagct
     2401 ccggcatttc cgagctgacc acgacgatcg cgaggccttc ggcggcgagc ttgaaaataa
     2461 gttggtagat ttcctgcttg gcgccgacat cgatgccgcg cgtcggctcg tcgagcagaa
     2521 gcacgcgcgg ctcggtcgcc agccatttgc cgatcacgat cttttgctgg ttgccgcccg
     2581 acagggtcga ggcgacctgc tcgatgccgg tgcagcgtac cgaaagacgg ccgacgacgt
     2641 cggcggcgag cgccgcctcg cgggcgaagg cgcgcaagcc gaaacgcgac atcgcgccga
     2701 tcgagggcaa agcgatattg tcgcggatcg tcgaacccaa atgcaggccg cgcgtcttgc
     2761 ggtcttcggt caccaaggcg acgccaagcc ggtaggcgtc cgccggcgag ttgatcgtcg
     2821 ccggcgcgcc gtcgatggtt atcgtcccgc cgcgccagcc gcggccggcg ccgaaaatcg
     2881 cctcgacgat ttcgctgcgg cccgagccga gcagcccgcc aatgccgaga atttcgccgc
     2941 gccgcaagtc gaagctgacg ccgtgcagca cgcgccgcca gccgcgcggc gtcgcgacgt
     3001 cgaaggtcag gttttcgacc gaaaggatcg gcgcggccga gccggccggt tcattgcggc
     3061 cgctcgtcgt cacgtcccgc ccgaccatcg ccgagatgat cccgtcgagc gaaatcttgt
     3121 cgatcggcga cgtgagcacg cgccgcccgt cgcgcagaac cgtgacgcgg tcggcgagcg
     3181 cgatgacctc gtcgaggcga tgcgacgtat aaatgatggc gacgccggac gcggccaatt
     3241 gccggaccac cttgaacagg gtctcgcatt ccgaggcgga gagcgccgag gtcggctcgt
     3301 ccatgatcag gatgcgcgta tcgagcgaca gcgccttggc gatctcgatc aattgctgct
     3361 cgccgacgcg caagctcccg acccgcgcct ccggatcgat cttgatgttg aggcggcgca
     3421 gcaacgcgcg ggcgacggcg acgatgcggc ggcggtcgat gacgaggccg gcgatcagcg
     3481 gctcgcggcc gagaaagatg ttttcggcga cgctcaattc cggaacgaga ttgagctcct
     3541 gatggatcat cgcgattccg gcgagctcgg cgtcgcgcac gccgttgaag gttacgtcct
     3601 gcccggcgac gctgatcgcg ccgagatagt cgccatggac gccggcgagg attttcatca
     3661 gcgtcgactt gccggcgccg ttctcgccca tcagcgcatg cacttcgccg catttcagat
     3721 cgaaatcgac gccctggagg gcccagacgc cagagaaagc tttggagacg cccttggcgg
     3781 ccaagacaat cgcgccgcgc tccgccgagg cctccggcgc ccgggcgagc ggctcctcag
     3841 agatttgcga cgcgccccgc caaagccgca ttgagccgct ccacccattg ccgaaagcgc
     3901 aacgcccgct tatcggtcgc cgccatcgtt gcttgccgcg tttctgatat gctagttata
     3961 ctagtgcaga tcggtcaagg ggttttgccg aggaggcgcg aaattgcttg ggggaaccaa
     4021 aggcgcccgc ttggtgggga ccgccatgcc aagcgcgacc ttccagagcg aggcggggcg
     4081 ccagtcaaag gccgatcgtg tctatctcag cttgaaacag gcgatcgttt ccggcgagct
     4141 tccgcccaac gctctgatcg acaagctgga gctcagcgcg cgctttcagg tctcgcgcct
     4201 ctccgtgacg gccgcggtca atcgtctcgc tttcgagaaa ctggtcttcg tcgagccgca
     4261 acgcggctcc tatgtcgcca aaatccggct atccgacgcc cggcaatgga tgctggtgcg
     4321 ccgcgcgctc gaaatggagg tggtcgccac ctgcgcgcgc gaattgccgg atgcggcgat
     4381 cgagcgcctc ggccgcaacg tcgcctatca gcgcaccgcg ctcgacgccg gcgacatgca
     4441 gggctttcat gagctcgaca ccgtgctcca tctgcaattg gtcgacgcgc tcggctgggg
     4501 ccgcgtcgcc gaaatcctcg atcccgtacg cacccatctc gagcgcgtcc ggcgcacgct
     4561 tctgcccgag cccggccgca tggagcgcac cttcgccgag caccaggaga tctatcgcgc
     4621 catcgccgcg cgcgagcctg acgctgcgcg cgaggcgatg gagcgccatc tcaaccaggt
     4681 gctgcgcgag ctggaaatat tcgtgacccg ccacccagga tttttcgagg cttaatcccc
     4741 gcagggcggg gagaagggag ctactccgcg gcctaggctt tcgtaccgag cgccccgagg
     4801 gcctggtcca agtcctcgat caggtcttcg acatcctcca agcccaccga taggcggatc
     4861 atgccgtcgc cgatgccgag ccgcgcgcgc gcatcgggct tcagccgctg atgggtcgtc
     4921 gtcgcgggat gggtgaccag gctcttggcg tcgccgagat tgttcgaaat gccgatgatg
     4981 gagagcgcgt tggcgacttt gaacgccgcc ggcttgccgc ccttgacttc gaacgtcacc
     5041 agcgtgccgc cgcccttcat ctgccgtttc gcgagggcat gttgcggatg atccgggcgg
     5101 cccggataga gcacgcgctc gatatgcgga tggccgctga ggaaatcggc gatcttctcc
     5161 gccgatcggg tttgcgcctt gacgcggatc gccagcgtct ccagcgattt cagcatcacc
     5221 caggcgttga agggcgacag cgacggtccg gtctggcgca ggaacgtatg gacatgggcg
     5281 gcgatgaaag cctcgctggc gaggatgacg ccgcccagcg tccggccttg gccgtcaata
     5341 tgcttggtcg ccgaatagac gacgcaatcc gcgccgagct gcagcggatg ctgcatcagc
     5401 ggcgtcgcga agacattgtc gacgaccagc gtcgcgccat gttcgcgcgc gatcttggcg
     5461 acggcggcga tgtcgatgat ttcgagcgtc ggattggttg ggctttccag gaacagggtc
     5521 ttggtgttgt cgcgcatggc cgcgcgccat tgctgcagat cggtcccgtc gacgagcgtc
     5581 gactgcacgc cgaaacgggg tagcaattcc tcgacgacat agagacaggc gccgaacagc
     5641 gcgcgcgcgg cgacgatgtg atcgccggcc ttgacctgac ccatcagcgc ggcggtgatc
     5701 gccgccatgc cgctcgccgt cgcgcgcgcc gcctcagcgc cttcaagcag cgccatgcgc
     5761 ttttcgaaca tcgccacggt ggggttggag tagcgcgaat agatgaagcc gggatcgtcg
     5821 cccttgaagc gggcttccgc ctcctccatc gtgtcgtaga gatagctttg cgttaagaac
     5881 agcgcttccg acgtctcctg gaagccggag cgcagcgagc cgccatggac aagcttggtc
     5941 gcctcgcgcg aaattttatg gtcgcgggtc tttctcgggg tcatcgcgtt ctcctgtccg
     6001 cgcaataacg cgtgagcggc gggcgtcaga cagggcgatt taaaagccgc ggtcttttag
     6061 ccccttgttt agcgtggcgg caagccgacc ggctcaaatg accacgttgt ccagagcttt
     6121 agcggggggg agccttggcg tcaatgcgac ggaagggtaa ggaggctgga gtttcccctc
     6181 ctccccgtga aacggggaga aggggagcgc gaccgaacgg gacccgatgc acgcgcgcag
     6241 ctccgagcct gaactcttcg acgcggtcgc gccgccggcg gacgacgcct atttcctcgc
     6301 gccttacggc ctgttcgcgc gccagaagat caaggcgatg gtcggccgca agatgatcgt
     6361 ctcgtcgcag acgatcgagg agagccagtt ccagccggcg agcctcgacc tgcgcctcgg
     6421 caaggaggcg taccgcatgc gcgcgagctt tctgcccggc cgcaaccgca gcgtcctcga
     6481 ccatcttgag agcctgaagc ccgagcgcat ttcgctgacc ggcgacggcg cggtgctgga
     6541 gaagggcatc gtctatatcg cggcgctgca ggagcggctc gaccttttgc cgagtctctc
     6601 ggcggccgcc aatccgaaaa gctcgaccgg ccggctcgat attttcaccc ggctcatcgt
     6661 cgatcgcggc gagcttttcg acgaggcgcc gctcggctat agcgggccgc tttacgccga
     6721 aatctcgccg cgctcgttca gcgtgcgcgt gcgcgaaggc tcgaagctga accagatccg
     6781 cttccgcagc cgcaattcgc agcaaaagag cctctcggac ttcgcgctgc ccgacaagga
     6841 cttgcggagc cggcacgccg cttcgccgct ggtcgatcgc gagctcgacc tgcgcgaggg
     6901 cctgatcttg cgcgtcggcc tcgaaggctc gcccggccag gtcgtcggct atcgggcgat
     6961 caagaacagc gaagtgatcg atgtcgaccg gcccggcgcc tatccggcgg ccgaattctg
     7021 ggagccgatc ctggcgcggc gcgacaagcg cctgatcctc gatccggatg agttctatat
     7081 cctggcgtcg cgcgagcgcg tgcaaatccc gcccgatctc gccgccgaga tggcgccgat
     7141 cgatccggcg atcggcgaat tccgcgtcca ttacgccggc tttttcgatc ccggtttcgg
     7201 ccacgccgcc gacggccggc cgagcgcccg cgccgtgctg gaagtacgca gccgcgatgt
     7261 ccccttcctg ctcgaagacg gccagccggt cggccgcctc gttttcgaaa agctcgccga
     7321 ccggacggac gcgctctacg gatcgaccgc cgtgtcgaac taccaagggc agggattgaa
     7381 gc
//
LOCUS       DATGJW010000505        18644 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_96591, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000505 DATGJW010000000
VERSION     DATGJW010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 18644)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 18644)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..18644
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96591"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..967
                     /gene="hydA"
                     /locus_tag="VKU03_15210"
     CDS             <1..967
                     /gene="hydA"
                     /locus_tag="VKU03_15210"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014329282.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HLJ72667.1"
                     /translation="LMVNDEEMFASFQRCAELGAMPLVHAENGDVVAMLQQKYLKAGI
                     TGPEGHALSRPPEVEGEAANRAIMIADQAGCPLYIVHVSCIQAHEAIRRARAKGMRVY
                     GEPLIQHLVLDESEYYNKDWTHAARRVMSPPFRDKKNQDDLWNGLRAGSLHVVATDHC
                     AFTTQQKMFGKDDFTKIPNGTGGLEDRMPVLWTYGVGTGRLTMNEFVAATSTNIARIL
                     NLYPKKGAIMPGADADIVVWDPKATKTISAKTQISAIDYNVFEGVKVRGLPRFTLSRG
                     EVPWRDGTLQVEPGRGKFVKRDPFPPEARALAAWKKHTEAKGVAR"
     gene            993..1811
                     /locus_tag="VKU03_15215"
     CDS             993..1811
                     /locus_tag="VKU03_15215"
                     /EC_number="7.6.2.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72668.1"
                     /translation="MLSPIVKQPRSAAAERVIDVADLSLTFETADGPVQALSEIDLAV
                     ERGEFVSFIGPSGCGKTTLLRAIADLETPTGGAIRVNGMSPSEARAKRAYGYVFQAPA
                     LYPWRTVARNIALPLEIMGFDKKERDERVAKGLDLVNLAGFGKKFPWQLSGGMQQRAS
                     IARALSFDPDLLLMDEPFGALDEIVRDKLNEQLLRLWDKTRKTVLFVTHSIPEAVFLS
                     TKIVVMSPRPGRVHDVIDCGFPRGRTLDIRETPEFLEVANRVRHGLREGHSYAD"
     gene            2007..2873
                     /locus_tag="VKU03_15220"
     CDS             2007..2873
                     /locus_tag="VKU03_15220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006934512.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72669.1"
                     /translation="MKNVLPVAIIVAAIVALWYVACLPMNAQLQRDLFANTDNTTYSF
                     SDFVTAEWNMDRPKLPAPHQIVAELDHTIFGLAPSSKRSLVYHAGITLQETILGFAIG
                     SALGILLAVLVVHLRGLDRSLMPWIVASQTIPIIALAPMIVVILNQFDITGVLPKAAI
                     AAYLSFFPVTVGMVKGLRSPDPLQLDLMRTYSATRAQTFFKLRAPASAPFFFASMKVA
                     VAAALVGAVVAEVTKSEDGGLGARLLAGSYYGQTVQIWAALFVAAACSAALVAIIGLA
                     DRITARRMGYAR"
     gene            2888..3646
                     /locus_tag="VKU03_15225"
                     /pseudo
     CDS             2888..3646
                     /locus_tag="VKU03_15225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012066595.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
     gene            3671..4663
                     /locus_tag="VKU03_15230"
     CDS             3671..4663
                     /locus_tag="VKU03_15230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181997.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ72670.1"
                     /translation="MKKMIFGLMATTALIAAGAAHAADKVTLQLKWVTQAQFAGYYVA
                     KDKGFYKDENLDVTIKPGGPDIAPVQVIAGGGADVVVDWMPDALSAREKGVPLVNIAQ
                     PFKHSGLELTCLASTGIKTPADLKNRTLGVWFYGNEYPFLNWMGKLGYKTDGSPGGVK
                     VLKQGFNVDPLLQKQADCISTMTYNEYLQVLDAGYKPNQLVVFKYEDEGVATLEDGLY
                     VMQDKLKDPAFVDKMARFVRASMKGWAWARANSDAAAKIVLDNDATGAQTEHHQTQMM
                     SEINKLTEGTDGKLDKADYERTVKTLLTGGSEPVITKEPTGAYTYAVTDKALAK"
     gene            5111..6388
                     /locus_tag="VKU03_15235"
     CDS             5111..6388
                     /locus_tag="VKU03_15235"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HLJ72671.1"
                     /translation="MAYLFTNVQIWDGENDERYPGEVLVENNRITAVARGHGQIHRDG
                     LAPVDGGGMTLMPGLVEGHCHLSFVNPLRNQDLGEIPPEEHLLRTCRNATLILNHGFT
                     SCYSAASAKLRIDVVVRQEIEDGYLAGPRYRAAGPEITVTGGLGDERKQHMHAESFGM
                     LADGPDEIRRAVRLCCREGVDNIKLNISGDEFVANARAEITSMSEIEVATAVEVAHEW
                     NKRVACHSRAAASVKRAVRNHVDCIYHCDFADEEALDMLEAAKDRVIVGPAFGLVHNA
                     VFEGEIIGMTKDVVEKMGLRRKLEATCRTYEQMRKRGMKVVVGGDYGFQLTPMGQNAR
                     DIGHFVKFFGYTPAEALRCATAVGGELMGHKGELGVIKPGALADILLVDGNPLADQSV
                     LVGPAHFAMIMKDGKMHRDPRNRQVEAGRIAAE"
     gene            6418..7935
                     /locus_tag="VKU03_15240"
     CDS             6418..7935
                     /locus_tag="VKU03_15240"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185040.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ72672.1"
                     /translation="MAQAALALEMLSISKSFPGVRALDGVSFACLRGEVHALCGENGA
                     GKSTLIKILGGVYRPDSGSLRLDGREVAFSHPVAARRAGISIIHQELSLLPERSVAEN
                     IYLGIEPKRFGMLDRATMRSGAGRLLSRLASTIAPDCRAGDLSIAQQQIVEIAKALAI
                     DARILVMDEPTAALDDVEAARLLDLVRRLSGEGVTIVYVSHRMPEIQAIADRVTVLKD
                     GRSVMTAPIAEAPTSRLVRAMVGRDLADFYPPRSPGSPGAAALAISGGSNGGLRGVDL
                     TLRAGEILGVAGLEGSGKRSLARAIFGDEPFESGTLEIRGEARIVKRPREAIALGIGC
                     LSDDRKREGLLLQQSVRDNAMLTQRAFAPSFRPPNARPMSIERTDAQLRKLDLRAAGF
                     DQEIRLLSGGNQQKTIIARWLAREPDILIFCEPTRGVDVATKAAIYKIMRDLAERGRA
                     ILMISSDLPEVIGVSDRILVMREGAIAGELPGGASEEDVMAIAVGHARETPAPAA"
     gene            7932..8918
                     /locus_tag="VKU03_15245"
     CDS             7932..8918
                     /locus_tag="VKU03_15245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72673.1"
                     /translation="MSAAQLAPAAIAVKRRGSLAPALLLAGSVALYIGAALWTGQTRM
                     LDTEGVVGLLQRMVALGIVALGQTFAILAGSIDLSVANLISVAAVLASFIMQGRPDMM
                     APAVAIVLAVSALVGLVNGALIARLQVNPLIATLGVGLILQGLLSASFTNFAGSVPPE
                     FQGVAYSGIGGVPYSVVALFVLGAFSAWLLRATRFGAHLYAVGGNPEGARLAGIRTDL
                     VMIGGHVICSVSAGLTGLYLASRLRSGAPWIGRDGVYDLESIAVVVIGGTLLAGGRGG
                     VAGTLAGVLLFATLDAVFNMLGVSAYPQLVLRGAIVILAVAAYTVRTKGHVA"
     gene            8915..9904
                     /locus_tag="VKU03_15250"
     CDS             8915..9904
                     /locus_tag="VKU03_15250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72674.1"
                     /translation="MTLALDRNRPGTRALALARKINVAIVVFLLLYLAIGLRQPTYLE
                     PAGVMNFLRRAAPLAILSSGQLFVLASGGFDLSVGSLVTFTVIGGSMLTANDPSKTWE
                     AIAILYAIGLAVGLINGAVVAYLKTPSIIATLGMLLSINGAAMMWSGGSPRGYLPDNF
                     RMFGRYVLHDVPVIKIFPLAIAVLAVCGALAYWGFHGTVFGRRILAVGDNPRAAELAG
                     VRVHAVRLSVFVISALSAVTAGIMLGGFGGVSVDVGLGLELQSIAACVVGGVQLMGGR
                     GAVIGAIAGALTLFALFTLLTLLGLPQPLKETAQGLILIAAVAVGAWRRRRAV"
     gene            9957..11090
                     /locus_tag="VKU03_15255"
     CDS             9957..11090
                     /locus_tag="VKU03_15255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018185043.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ72675.1"
                     /translation="MRKMIMRLALGAALASFAGAALAQNFDDPKEFQAAKELLAMQPE
                     GPAGKPWEQHLGGKEVDTTKYKKPGPYRVCFSNAGVDNPWRVVGWTDMQAQVDVLKSD
                     IKSFTAADAEGKDDKQISDINAFVSSGNCDILIVSPNTTAALTPAVEAACKKLPVVVF
                     DRGVLTDCPVTFVNPIGGYGWGIQTANFIVSKLPKGGKVLALRILPGVDVLETRWSAA
                     NRIFKEHGVQVVGAEFTNGDNAKTKSIVEDYINRFGKIDAVWMDAGATAVAALEAFED
                     AGQPYPIITGEDQEDFLMKWKDKHLTALAPTYPTYQWRSPIIAAVKILKGEPVIGPKW
                     KLPQPVITEADLDKYVDPKMPPLHYAMCGCKDLPGYPQRWGGK"
     gene            complement(11115..11681)
                     /locus_tag="VKU03_15260"
     CDS             complement(11115..11681)
                     /locus_tag="VKU03_15260"
                     /inference="COORDINATES: protein motif:HMM:NF020897.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ72676.1"
                     /translation="MGDEITTETAGVALIADLLAHLATGGGAAAAGFAKQVGAARSTV
                     YQAIERLEQTGFVERDARGVLAPGANAARLGLASLGLAALLPAAEALLPALRNEADAT
                     VSLMSDAQGTEIVLMRRAARWDTRESAGKELEAAIAEKARLRLRLRPRAGEIERRAAR
                     LCLDRTAEALRRALAEAADKAERIAGGA"
     gene            complement(11671..12840)
                     /locus_tag="VKU03_15265"
     CDS             complement(11671..12840)
                     /locus_tag="VKU03_15265"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ72677.1"
                     /translation="MAARREALLQAEDQRRLAPRPRPVHRARGAGCALSRCAAAAQWS
                     EAPLGRRPRFRIGFSNADMNNLWRVALVHSVEYGAVKYRSLVRSLAVRHAEGCAERQA
                     RDIGDLLDKGVDGLIVSAHRAPALAAAFAEAARRGVPLVLVDRGDEDAPHASFVTCDD
                     EVIGRVTAIWLAEKLEGRGDVLLLPGLQAAAPARARLGAALRTLRDFPQIRILDICWS
                     DWRQDLGRAIVEAVLARGARIDGVWCDSGLQAVGSLKAFIAAERRIPPHTGGDLNLAY
                     KLAIRHDAPLAAVDYPPAMGLKAVETLLDFLRGRSVAKRIHVPTEIVVTRGHATSSVR
                     PDLWADQHVRWDLPDDLILASGLGPSYDPRRFRVNYVGTRYNRSAAHPSERADGR"
     gene            12766..13134
                     /locus_tag="VKU03_15270"
     CDS             12766..13134
                     /locus_tag="VKU03_15270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72678.1"
                     /translation="MDGARPRRQPALILGLKQRLAPRRQSEPNGAEGEPAAAPAIDDP
                     PRGENARDPMQGGARRAKPHQGLGDRDRPLSGNQELEKADRNVLAALAHAAGSRVFDA
                     KFEDIEADIRDATVHAECSF"
     gene            13329..13988
                     /locus_tag="VKU03_15275"
     CDS             13329..13988
                     /locus_tag="VKU03_15275"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ72679.1"
                     /translation="MAKKLQPTLSKMLQVERAFYDHGYSELSMRGLAKACDLSTRALY
                     YYFSSKEEAFRATCRFRNELALNTCFAAARRRWDEGGDALDIIAELINIRYGDTRRMA
                     NASQHLVELNAETFKRCNDIITEVALHFEAALAKFIIEMEEGGLLQLRADATPEQLAQ
                     ALANGARGVNQRLPPAAPDALASCYREICCFVLFGGAEIAAANQPPRRQAPKRAGDPR
                     S"
     gene            13985..14320
                     /locus_tag="VKU03_15280"
     CDS             13985..14320
                     /locus_tag="VKU03_15280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72680.1"
                     /translation="MSGEAPPLDPESAEAAAGRAMIADALDRLPLTKLHVAILALCAM
                     GLAADIGEVALSNTFSALLLAPPSNAPRGAVSLHLAAVFAGGAIGAPLFGWPADKSGR
                     RAVAGKPVD"
     gene            14418..16646
                     /locus_tag="VKU03_15285"
     CDS             14418..16646
                     /locus_tag="VKU03_15285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0098561 - methyl accepting chemotaxis
                     protein complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methyl-accepting chemotaxis protein"
                     /protein_id="HLJ72681.1"
                     /translation="MLKSLTIKARIALISVVFVAGLAVIGGANLVGSRNVDEAFADAK
                     AYAALQVKAGKIAETAIGLKGVARDVRYRHESVELAKFADGLARLTGLVDDLAAAPRG
                     QAVAQQISDLKNQIAALAKQFATVQGLQKPLGDSGSAGLADRADSTGASLVDMLKANI
                     AEQDSLEAERLLAATFAMRRAQSEYARTFDDSLTGEWEVGYGRFDSTLKNAEIDAATK
                     RLIASTFKDYAKSFKAASAAETDYMRAAERVSGGLDLIAPVLQELDAKASQDAAAAGQ
                     RLAASEALTKKIIFAAMALALAGGLIAAAIVARTTAKPLGQLRDAMLGLASGDHATEV
                     PALGRADEIGQMAKAVQSFKEAALDRERLERDAAAQREASEAERRGAEAERAARERDQ
                     QRVVAVIAHGHEQLANGNLTFRIDETFPPAYQKMKDDFNAAIAQMQQAMSVISATTQG
                     IRANAGEVSQASDDLSRRTEQQAASLEESAAALSEITATVKKAAEGANLASRAVAAAD
                     SDARKGAEVVRHAVAAMDAIAKSAAKINQIIGVIDEIAFQTNLLALNAGVEAARAGDT
                     GRGFAVVAAEVRALAQRSAEAAKEIKGLISASTNQVNQGVKLVGESGQSLERVMAQVA
                     DLNRIILEIAEGARAQAAGLEEVNAAIGQMDKMTQQNAAMVEETTATSHSLSQETSDL
                     SSLIGAFQVGGAAEEAQLRHELQNAAPHAFSAPRNGAVRPRGTPPASGPRPRGPTLVR
                     SH"
     gene            16835..17566
                     /locus_tag="VKU03_15290"
     CDS             16835..17566
                     /locus_tag="VKU03_15290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006021996.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0008097 - 5S rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L25/general stress protein
                     Ctc"
                     /protein_id="HLJ72682.1"
                     /translation="MAATKQLAAVARSGIGKGAARSVRRAGRVPAVIYGGGETAAPIA
                     LDYKEVNQLINAGHFLTTIFELDVEGRKERVLPRDYQLDVVTDRPLHVDFLRLKAGSR
                     VRIATPVHVVNQDKAPGIKRGGALNLVLHAIELWAPAEAIPDSITIDLTGLDIHDSVH
                     LSNIQLPEGCKPVDTRTDFTIATVVPPTVYVEEAPVAAAAATTAAAAPAAGAAAPAPG
                     AAPAAPAAAAPAAPAKGAAAPAAKK"
     gene            17618..18220
                     /gene="pth"
                     /locus_tag="VKU03_15295"
     CDS             17618..18220
                     /gene="pth"
                     /locus_tag="VKU03_15295"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020186003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HLJ72683.1"
                     /translation="MILLAGLGNPGARHAKNRHNIGFMAIEAIAKAHRIGPFRARFQG
                     AAAEGLIGGERALLLMPQTYMNDSGRSLGEAMRFHKLDLADIVVIHDELDLAPAKVRV
                     KVGGGNAGHNGLRSISAHIGNEYKRVRLGIGHPGDKALVHQYVLSDFFKAETGWVAAV
                     CDAVARAADLLARGEDASFQNKVHLAVNAAGFGENAPAED"
     gene            18489..>18644
                     /locus_tag="VKU03_15300"
     CDS             18489..>18644
                     /locus_tag="VKU03_15300"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020091488.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HLJ72684.1"
                     /translation="MPASDRLLVLVRHGQSDWNLKNLFTGWRDVDLSPKGVEEAREAG
                     RRLKRQGL"
BASE COUNT         3122 a         6397 c         6084 g         3041 t
ORIGIN      
        1 gctaatggtc aatgacgagg aaatgttcgc ctcgttccag cgctgcgccg agctcggcgc
       61 catgccgctc gtccacgccg agaatggcga cgtcgtcgcc atgctgcagc agaaatacct
      121 gaaggccggc attaccggcc ccgagggcca tgcgctgtcg cggccgccgg aagtcgaagg
      181 cgaagccgcc aaccgggcga tcatgatcgc cgaccaggcc ggctgcccgc tctatatcgt
      241 gcacgtctcg tgcatccagg cgcatgaggc gatccggcgg gcccgcgcca agggcatgcg
      301 cgtctatggc gagccgctga tccagcatct cgtgctcgac gagagcgaat attacaacaa
      361 ggactggacc catgcggcgc ggcgcgtgat gtcgccgccc ttccgcgaca agaagaacca
      421 ggacgatctc tggaacgggt tgcgcgccgg ctctctccac gtcgtcgcca ccgaccattg
      481 cgccttcacc acgcagcaga agatgttcgg caaggacgac ttcaccaaga tcccgaacgg
      541 caccggcggt ctcgaagacc gcatgccggt cctctggacc tatggcgtcg gcaccggccg
      601 gctgacgatg aacgagttcg tcgccgccac ctcgaccaac attgcgcgca tcctcaatct
      661 ctatccgaag aagggcgcga tcatgccggg cgccgacgcc gatatcgtcg tctgggatcc
      721 gaaggccacc aagacgatct ccgccaagac gcagatttca gcgatcgact acaatgtctt
      781 cgagggcgtc aaagtccgag gtctgccgcg cttcacgctg tcgcgcggcg aggtgccgtg
      841 gcgcgacggc acgcttcagg tcgagcccgg ccgcggcaaa ttcgtcaagc gcgatccgtt
      901 cccgcctgaa gcgcgcgccc tcgcggcgtg gaagaagcat accgaagcaa agggcgtcgc
      961 ccgatgatca gccgcgcggc gccgggagcg tcatgctgag cccaatcgtc aagcagccgc
     1021 gaagcgccgc cgccgagcgg gtcatcgacg tcgccgatct gtccttgacg tttgagaccg
     1081 cggacggccc ggttcaggcg ctgagcgaga tcgacctcgc cgtcgagcgc ggcgagttcg
     1141 tctcgttcat cggcccctcg ggctgcggca agacgacgct tttgcgcgcc atcgccgatc
     1201 tcgaaacgcc gaccggcggc gcgattcgcg tcaacggcat gagcccttcc gaggcgcgcg
     1261 ccaagcgcgc ctatggctat gtgttccagg cgccggccct ctatccctgg cgcacggtcg
     1321 cgcgcaatat cgcgctgccg ctcgaaatta tgggcttcga caaaaaggag cgcgacgagc
     1381 gcgtcgccaa aggcctcgac ctcgtcaatc tcgccggctt cggcaagaaa tttccctggc
     1441 agctttccgg cggcatgcag cagcgcgcct cgatcgcgag agctctatcg ttcgatcccg
     1501 atctgctctt gatggacgag ccgttcggcg cgctcgacga gatcgtgcgc gacaagctca
     1561 acgagcaatt gctgcgcctc tgggacaaga cgcggaagac cgtgctcttc gtcacccact
     1621 ccattccgga ggcggtgttc ctatcgacca agatcgtcgt catgtcgccg cgccccggcc
     1681 gcgtccacga cgtgatcgat tgcggctttc cgcgcggccg cacgctcgac atccgcgaga
     1741 ccccggaatt cctggaggtc gccaatcgcg tgcgccatgg gctgagggaa gggcactcgt
     1801 atgcggattg agcgaggccg cgatcctcgg gctgtttctc tcctcccccg tcgcagacgg
     1861 gggaggggga ccgcgcgtca gcgcggtgga gggggccgcg gcgccgggga atttgcttcg
     1921 accgccgtcg ccccctccgc catgcttcgc atggtccccc tcccccgctt tgcgggggag
     1981 gatggcttcc gcgaagagcg aggccgatga agaacgtcct gcccgtcgcc attatcgtcg
     2041 ccgcaatcgt cgcgctctgg tacgtcgctt gtctgcccat gaacgcgcaa ttgcagcgcg
     2101 atcttttcgc caatacggac aatacgacct attccttctc cgatttcgtg accgcagagt
     2161 ggaacatgga ccggcccaag ctgccggcgc cgcaccagat cgtcgccgag ctcgaccata
     2221 cgattttcgg cttggcgccg tcctccaagc gcagcctcgt ctaccatgcc ggcatcacgt
     2281 tgcaggagac gatcctcggc ttcgccatcg gctcggcttt gggcattctc ctcgccgtgc
     2341 tggtcgttca cttgcgcggc ctcgatcgat cgctgatgcc gtggatcgtc gcctcgcaga
     2401 ccatcccgat catcgcgctc gctccgatga tcgtcgtcat tctcaaccag ttcgacatta
     2461 ccggcgtttt gcccaaggcg gcgatcgccg cctatctctc cttcttcccg gtcacggtcg
     2521 gcatggtcaa aggcctgcgc tcgcccgatc ctctgcagct tgatttgatg cgcacctatt
     2581 cggcgacgcg cgcgcagact ttcttcaaat tgcgcgcccc ggccagcgcg cccttcttct
     2641 tcgccagcat gaaggtggcg gtcgcggcgg ctctcgtcgg cgcggtggtg gcggaagtca
     2701 ccaagagcga ggatggcggc ctcggcgcgc gtctgctcgc cggctcctat tacggccaga
     2761 ccgtgcagat ttgggcggct ctattcgtgg cggcggcgtg ttcggcggct ttggttgcga
     2821 ttatcggcct ggccgaccgc atcaccgccc gccgcatggg ctatgcgcga tgaggagccg
     2881 cgcgctcgcc aatctcgccg ccccgacgct gttcggcgtc ggcgttctcc ttttgtggga
     2941 agcggtggtt cgcggcttca atgtcccgcg ggtcatcttg ccggcgccga gcgcgatcat
     3001 cgcccgcctc gccacctcgg cgccgaccct cgtcgcagac tttttgcaaa ccattcaggg
     3061 cgtgctcgcc ggctatttca tcggctgcgc cgccggtctc atcgtcgcca tcctgatcga
     3121 ccgcacgccg ttcctgcagc gcggcctgct gccgctcggc aatctggttt cggctctgcc
     3181 gatcgtcggc atcgcgccga tcatggtgat gtggttcggc ttcgactggc agtcgaaggc
     3241 ggcggtggtg gtggtgatga ccttcttccc gatgctggtc aacgccgtcg ccggcctcgc
     3301 ctcggccggg tcgatggagc gcgatctgat gaagacctac ggcgcgacct acggccagac
     3361 cttgaccaag ctgcgcctgc aagcggcctt gcccttcatc ttcaatgcgc tgaagatcaa
     3421 ttcgacgctg gcgatgatcg gggcgatcgt cgccgaattt ttcggcacgc cggtcgtcgg
     3481 catgggcttt cgcatcaata ccgaggttgg acgcatgaat atcgacatgg tctgggcgac
     3541 gatcgtcgtc gccgcggtga ccggctcgtt gttctatggc gtcgttgcgc tgatcgagcg
     3601 ccaagtcacg ttctggcatg catcctaccg ccagagaccc gcgtgaggag ctaaggaggg
     3661 agaggtacaa atgaagaaga tgatctttgg tttgatggcg acgacggctc ttatcgccgc
     3721 cggcgccgcg catgccgcgg acaaggtgac gctgcagctc aaatgggtga cccaggcgca
     3781 attcgccggc tattacgtcg ccaaggacaa gggcttctac aaggacgaga atctcgacgt
     3841 gacgatcaag cccggcgggc cggatatcgc gccggtgcag gtgatcgccg gcggcggcgc
     3901 cgatgtcgtc gtcgactgga tgccggatgc gctgtcggcg cgcgaaaaag gcgtgccgct
     3961 ggtcaatatc gctcagccct tcaagcattc gggcctcgaa ttgacctgcc tggcctcgac
     4021 cgggatcaag acgccggccg acctcaagaa tcgcaccctc ggcgtctggt tctacggcaa
     4081 tgaatatccg ttcctgaact ggatgggcaa gctcggctac aagaccgacg gctcgccggg
     4141 cggcgtcaag gtgctcaagc agggcttcaa cgtcgatccg ctgctgcaga agcaggccga
     4201 ttgcatctcg accatgacct ataacgaata tttgcaggtc ctggatgcag ggtacaagcc
     4261 caatcagctc gtcgtcttca aatacgaaga cgaaggggtc gcgacgcttg aggacggcct
     4321 ctatgtgatg caggacaagc tcaaggatcc ggccttcgtc gacaagatgg cgcggttcgt
     4381 gcgcgcctcg atgaagggct gggcctgggc gcgcgccaat tccgacgccg ccgccaagat
     4441 cgtgctcgac aatgacgcga ccggcgccca gaccgagcac caccagacgc agatgatgag
     4501 cgagatcaac aagctcaccg aaggcaccga cggcaagctc gacaaggccg actacgagcg
     4561 cacggtcaag acgctgctga ccggcggctc ggagccggtc atcaccaagg agccgacggg
     4621 ggcctacact tacgccgtga ccgacaaggc cctggcgaag taagacggct gcttcttctt
     4681 aaatccggcg cggcgcggtc gatgcgccgc gccaacgcgt tacgttcgac gccacgctgg
     4741 ccgaccgccg tcaatccgcc atagcgcctc gaacgctggt gaatacgacc gaacgcctac
     4801 gaggcgctat ggcgggtgct ccgcataacg cttcgcccgt tcgggcgacg gcgatcgccc
     4861 ggcgcggcag gatggggcgc tttttgcgcc ctctcgaccc tgtttggtcg aaacgctcac
     4921 agttggcgct gcccgccccc tcccacccga cgacccgcgg gccgctctcg cttcgcccga
     4981 atgtctcaaa tagtggattc caggcccgga ttgccggtag cttgccgagc cgggcttttc
     5041 tagactggcg gccagccggc ggaagaccgg cggcgcccaa ggcggcgcgt cgcggaggag
     5101 gacgtcagcg atggcctatc ttttcaccaa cgtgcaaatc tgggacggcg agaacgacga
     5161 gcgctatccg ggcgaggtcc tcgtcgagaa caaccgcatc acggcggtcg cgcgcggcca
     5221 tggccaaatc catcgggacg gcctcgcgcc ggtcgacggc ggcggcatga cgctgatgcc
     5281 aggcctggtc gagggccatt gccatctctc cttcgtcaat ccgctgcgca accaggacct
     5341 cggcgagatc ccgcccgagg agcatttgct gcgcacctgc cgcaacgcga cgttgatcct
     5401 caatcacggc ttcacctcct gctattcggc ggcttcggcc aagctgcgca tcgacgtggt
     5461 cgtgcgccag gagatcgagg acggctatct ggcgggcccg cgctaccgcg ccgccgggcc
     5521 ggaaatcacg gtgaccggcg gcctcggcga cgagcgcaag cagcatatgc acgccgagag
     5581 cttcggcatg ctggccgacg gccccgacga aatccgccgc gcggtgcgcc tgtgctgccg
     5641 cgaaggcgtc gacaacatca agctcaacat atcgggcgac gagttcgtcg ccaatgcgcg
     5701 cgccgaaatc acctcgatga gcgaaatcga ggtcgcgacc gccgtcgagg tcgcgcatga
     5761 atggaacaag cgcgtcgcct gccattcgcg cgccgccgcc tccgtcaagc gggcggtgcg
     5821 caaccatgtc gattgcatct atcactgcga tttcgccgat gaggaggcgc tcgacatgct
     5881 cgaagccgcc aaggaccggg tgatcgtcgg gcccgctttc ggcctcgttc acaatgcagt
     5941 cttcgagggc gagatcatcg gcatgaccaa ggacgtcgtc gagaagatgg gccttcgccg
     6001 caaactcgag gcgacctgcc gcacctacga gcagatgcgc aagcgcggca tgaaagtggt
     6061 ggtcggcggc gattacgggt tccaactgac gccgatgggg cagaacgccc gcgacatcgg
     6121 ccacttcgtc aagttcttcg gctatacgcc tgccgaggcg ctgcgctgcg cgaccgcggt
     6181 cggcggcgaa ttgatggggc ataagggcga gctcggcgtg atcaagccgg gcgcgctcgc
     6241 cgacatcctg ctggtcgacg gcaatccgct cgccgatcaa tcggtgcttg tcggccccgc
     6301 gcatttcgcg atgatcatga aggacggcaa gatgcatcgc gatccgcgca accgccaggt
     6361 cgaagccggg cgaatcgccg ccgaataaag cgcgcggcga aagggaggac ggtcaaggtg
     6421 gcgcaggcgg cgctcgctct cgaaatgctc tcgatcagca agagctttcc cggcgtgcgg
     6481 gcgctcgacg gggtcagctt cgcctgcttg cgcggcgagg tccatgcgct gtgcggcgag
     6541 aacggcgccg gcaaatcgac cttgatcaag attctcggcg gcgtctatcg gcccgatagc
     6601 ggcagcctgc ggctcgacgg acgggaggtc gccttttcgc atcccgtcgc ggcgcggcgg
     6661 gccgggatca gcatcattca ccaggagctc agcctgctgc cggagcgctc ggtcgccgag
     6721 aacatctatc tcgggatcga gccgaaacgg ttcggcatgc tggatcgcgc cacgatgcgc
     6781 agcggcgccg gccgcctgtt gagccgcctc gcctcgacca tcgcgccgga ctgtcgggcg
     6841 ggcgatcttt cgatcgcgca gcagcagatc gtcgaaatcg ccaaggcgct ggcgatcgac
     6901 gcgcgcattc tggtgatgga cgagccgacc gccgcgctcg acgacgtcga ggccgcgcgc
     6961 ctgctcgatc tcgtccgccg cctcagcggc gagggcgtga cgatcgtcta tgtctcgcac
     7021 cgcatgcccg agattcaggc gatcgccgat cgcgtcaccg tgctcaagga cggccgctcc
     7081 gtgatgacgg cgccgatcgc ggaggcgccg acgagccggc tggtgcgggc gatggtcgga
     7141 cgcgatctcg ccgatttcta tccgccgcgc tcgccaggat cgccgggagc ggccgcgctg
     7201 gccataagcg gcggctcgaa cggcggcctg cgcggcgtcg acctgacgct tcgcgccggc
     7261 gaaattctcg gcgtcgccgg cctggaaggc tcgggcaaaa gatcgctggc ccgggcgatt
     7321 ttcggcgacg agccgttcga gagcggaacc ctcgaaatcc gcggcgaggc gcgcatcgtc
     7381 aagcggccgc gcgaggcgat cgcgctcggc atcggctgcc tgtcggacga ccgcaagcgc
     7441 gaagggctgc tcctgcagca atcggtgcgc gacaacgcca tgctgacgca aagagctttc
     7501 gcgccgtcgt ttcgcccgcc caatgcgcgg ccaatgtcga tcgagcgaac cgacgcccag
     7561 cttcgcaagc tcgacctgcg cgccgccggc ttcgatcagg aaatccgcct gctttccggc
     7621 ggcaaccagc agaagacgat catcgcgcgc tggctcgcgc gcgagccgga catcctgatc
     7681 ttctgcgagc cgacgcgcgg cgtcgacgtc gcgaccaagg cggcgatcta caagatcatg
     7741 cgcgatctcg ccgaacgcgg acgggcgatc ctgatgatct cgtcggactt gccggaagtg
     7801 atcggggtca gcgaccgcat cctggtgatg cgggaaggcg cgatcgccgg cgaattgccg
     7861 ggcggcgcga gcgaagagga cgtgatggcg atcgcggtgg gccacgcccg cgaaacgccc
     7921 gcgccggccg cgtgagcgcc gcgcagctcg cgccggccgc gatcgccgtc aagcggcgcg
     7981 gctcgctcgc cccggcgctg ctgctcgccg gctcggtcgc gctctatatc ggcgccgcct
     8041 tgtggaccgg ccagacccgg atgctcgata cggagggcgt cgtcggcctg ctccagcgca
     8101 tggtggcgct cggcatcgtc gcgctcggcc agactttcgc gatcctggcc ggctcgatcg
     8161 acctctcggt cgccaatctg atcagcgtcg cggccgtgct cgcctccttc atcatgcaag
     8221 gccggccgga catgatggcg cccgccgtcg cgatcgtgct cgccgtctcc gccctggtcg
     8281 gcctcgtcaa cggcgcgctg atcgccaggc tccaggtcaa tccgctcatc gcgacgctcg
     8341 gcgtcggctt gattctgcaa ggcctgctct ccgccagctt caccaatttc gccggctcgg
     8401 tgccgccgga attccagggc gtcgcctata gcgggatcgg cggcgtacct tattccgtcg
     8461 tggcgttgtt cgttctcggc gcgttctcgg cctggctgct tcgcgcgacg cgattcggcg
     8521 cccatcttta tgcggtcggc ggcaatccgg aaggcgcgcg cctcgccggc atccgcaccg
     8581 atctcgtcat gatcggcggg cacgtgatct gctccgtttc ggctggcctg accggtctct
     8641 atctcgccag ccggttgcgg tcgggcgcgc catggatcgg ccgcgacggc gtctatgatc
     8701 tcgaatcgat cgccgtcgtg gtgatcggcg ggaccttgct ggcgggcggg cgcggcggcg
     8761 tcgccggcac gctggccggc gtgttgctct tcgcgacgct cgacgccgtg ttcaacatgc
     8821 ttggcgtctc ggcctatcct cagctcgtgc tgcgcggcgc gatcgtgatc ctggcggtgg
     8881 cggcttacac cgtccggacg aaagggcatg tcgcgtgacc ctcgccctcg accggaatcg
     8941 cccgggaacc cgcgccttgg cgctggcccg caagatcaac gtcgccattg tcgttttcct
     9001 gcttctttac ctcgcgatcg gccttcgcca gccgacctat ctcgaaccgg ccggcgtgat
     9061 gaacttcctc cgccgcgccg cgccgctcgc cattctttcg tcgggccagc tcttcgtgct
     9121 ggcgagcggc gggttcgatc tttccgtcgg ctctctggtc accttcaccg tgatcggcgg
     9181 atcgatgctg accgccaacg atccgagcaa gacctgggag gcgatcgcga ttctctacgc
     9241 gatcggtttg gccgtcggcc tgatcaacgg cgcggtggtc gcctatctca agacgccgtc
     9301 gatcatcgcg acgctcggca tgctgctttc gatcaacggc gccgcgatga tgtggtcggg
     9361 cggatcgccg cgcggctatc tgccggacaa tttccgcatg ttcggccgct acgtgctcca
     9421 cgacgtgccg gtcatcaaga ttttcccgct cgccatcgcc gtgctcgccg tctgcggcgc
     9481 gctcgcttat tggggcttcc acggcacggt gttcggccgc cgcatcctcg cggtcggcga
     9541 caatccgcgc gcggcggagc tcgccggcgt gcgcgttcac gccgtccgcc tcagcgtttt
     9601 cgtcatctcc gcgctttccg cggtcaccgc cggcattatg ctcggcggtt tcggcggcgt
     9661 gtcggtcgat gtcggattgg gcttggagct gcaatcgatc gccgcctgcg tcgtcggcgg
     9721 cgtgcaattg atgggcggcc gcggcgcggt gatcggcgcg atcgccggcg ccctcacctt
     9781 gttcgcgctg ttcacgctgc tcaccttgct cggcctgccg cagccgctca aggagacggc
     9841 gcagggcctt attctcatcg cggcggtggc ggtcggcgct tggcgccgcc gccgcgccgt
     9901 ttagcggggc gtccgcccca tgaagcgccg cgagtcggca aagggaggaa gacgatatgc
     9961 gtaagatgat catgcgcctc gcgctcggcg cggcgctggc gagcttcgcc ggcgcggctc
    10021 tggcgcagaa tttcgacgac ccgaaggagt ttcaggcggc gaaggagctc ctcgccatgc
    10081 agccggaagg cccggccggc aagccctggg agcagcatct cggcggcaag gaggtcgaca
    10141 ccacgaaata caagaagccc ggtccgtacc gcgtctgctt ctccaatgcc ggcgtcgaca
    10201 atccctggcg cgtcgtcggc tggaccgaca tgcaggcgca agtcgacgtg ctgaaatccg
    10261 acatcaagtc cttcaccgcc gccgacgccg aaggcaagga cgacaagcag atttcggaca
    10321 tcaacgcctt cgtctcgagc ggcaattgcg acatcctgat cgtctcgccg aacacgacgg
    10381 cggcgctgac gccggcggtc gaagccgcgt gcaagaagct gccggtcgtc gtcttcgacc
    10441 gcggcgtgct gaccgactgc ccggtgacct tcgtcaatcc gatcggcggc tatggctggg
    10501 gcatccagac cgccaatttc atcgtctcga aactgccgaa aggcggcaag gttctggcgt
    10561 tgaggatctt acccggcgtc gacgtgctcg agacgcgatg gtcggcggcc aaccgcatct
    10621 tcaaggagca cggcgtgcaa gtcgtcggcg ccgaattcac caacggcgac aacgccaaga
    10681 ccaagtcgat cgtcgaggat tacatcaacc gcttcggcaa gatcgacgcg gtgtggatgg
    10741 atgcgggcgc gacggcggtg gcggcgctgg aggcgttcga ggacgccggc cagccctatc
    10801 cgatcatcac cggcgaggat caggaagatt tcctgatgaa atggaaggat aagcatctga
    10861 ccgccttggc gccgacttat ccgacctatc agtggcgctc gccgatcatc gccgcggtca
    10921 agattttgaa aggcgagccg gtgatcgggc cgaagtggaa actgccgcag cccgtgatca
    10981 ccgaggccga tctcgacaaa tatgtcgatc cgaaaatgcc gccgcttcat tacgcgatgt
    11041 gcggctgcaa ggatctcccc ggttatccgc agcgctgggg cggcaagtaa ccgccgtaaa
    11101 ccgccggagg ggcgtcaagc ccctccggct attctctccg ctttgtccgc agcttcggcg
    11161 agcgctcgcc gcagcgcctc ggcggtacgg tcgagacaaa gcctcgcggc gcggcgctcg
    11221 atctcgccgg cgcgcgggcg caaccgcagg cgaaggcgag ccttttcggc gatcgccgcc
    11281 tccaattcct ttcccgcgct ctcgcgcgta tcccaacgcg ccgcgcggcg catcagtacg
    11341 atttccgtcc cttgcgcatc cgacatgagc gaaacggtcg cgtcggcctc gttgcgcaga
    11401 gccggcagaa gcgcttcggc ggcgggcagc agggcggcga ggccgagcga ggcgaggccg
    11461 agccgcgccg cgttcgcgcc cggcgcaaga acgccgcgcg cgtcacgctc gacgaagccg
    11521 gtctgctcca gccgctcgat cgcctgatag acggtcgagc gggccgcgcc gacttgcttg
    11581 gcgaagcccg ccgcagcggc gcctccgccc gtagcgagat gggcgagaag atcggcgatc
    11641 agcgcgacgc ccgccgtttc ggtcgtgatc tcatcgccca tcggcgcgct ccgagggatg
    11701 agcggcggag cggttatagc gcgtgccgac atagttcacg cgaaaccgcc gcgggtcgta
    11761 ggacgggcca agcccgctcg ccagaatgag gtcgtccggc aagtcccagc gcacatgctg
    11821 gtcggcccaa agatcgggcc gcaccgagga ggtggcgtgg ccgcgcgtca ccacgatttc
    11881 cgtcggaacg tggatgcgct tggcgacgga gcggccacgg agaaaatcca gcaaggtctc
    11941 gaccgccttc aggcccatgg ccggagggta atccactgcg gcgagcggcg cgtcgtggcg
    12001 gatggcgagc ttgtaggcga ggttgaggtc gccgccggta tgcggcggaa tgcgccgctc
    12061 ggcggcgatg aacgctttca gcgagccgac cgcctgaagc cccgaatcgc accagacgcc
    12121 gtcgatcctc gcgccgcgcg ccagaacggc ttcgacgatc gcgcggccga ggtcttggcg
    12181 ccagtcgctc cagcaaatgt cgaggatgcg gatctgcgga aaatcgcgca gcgttcgcag
    12241 cgccgcgccc aaccgcgccc ttgccggcgc ggccgcctgc aagccgggca gcaacagcac
    12301 gtcgccgcgc ccctccaact tttcggcgag ccaaatcgca gtgacccgtc cgatgacctc
    12361 gtcgtcgcag gtcacgaagc tcgcatgcgg cgcgtcctcg tcgccgcgat cgaccaggac
    12421 cagggggacg ccgcgccgcg ccgcctcggc gaaggcggcg gccagcgccg gggcgcgatg
    12481 cgcgctgacg atgagcccgt cgacgccctt atcgaggaga tcgccgatat cgcgcgcttg
    12541 acgctcggcg catccttccg cgtgcctgac cgccaaggag cggaccagcg agcgatattt
    12601 caccgcgccg tattcgacgc tatgcacgag agcgacgcgc cagagattgt tcatatcggc
    12661 gttcgagaac ccgatcctga agcgcggcct gcgcccaagc ggcgcctcgc tccattgggc
    12721 cgccgccgcg catcgggaaa gggcgcaacc cgcgccgcgc gcgcgatgga cggggcgagg
    12781 ccgcggcgcc agccggcgct gatcctcggc ttgaagcagc gcctcgcgcc gcgccgccaa
    12841 agcgagccca acggggccga aggcgagcca gccgcggcgc cggcaatcga cgatcccccg
    12901 cgcggcgaaa atgcgcgcga tccgatgcaa ggtggcgcgc ggcgcgccaa gccgcatcag
    12961 ggcctcggcg accgcgatcg gccgctcagc ggcaatcaag agctggagaa ggccgatcgc
    13021 aatgttttgg ccgcgctggc gcatgcggcc ggttcacgcg tcttcgacgc aaaattcgag
    13081 gatatcgaag ccgatatccg cgacgcgacc gttcacgccg aatgttcgtt ctgagaacgg
    13141 cgaaaccgaa atggacgtgg ccgctgcgaa acccattgcg tacccgcggc tttcctcgca
    13201 aggcgacggc tccgggttaa agagtttcca cggccctgac aagctcgggg cgcgccgcgc
    13261 cttgcctcgg caaggccgag atattatgca ttccgccgtt aagaggtcgt ctgtcgtcga
    13321 aaggatcaat ggcgaagaag ctccaaccta cgctcagcaa gatgctgcag gtcgagcgcg
    13381 cgttttacga ccacggctac agcgaattga gcatgcgggg cctcgcgaaa gcctgcgatc
    13441 tctcgacgcg cgctctctac tactatttca gcagcaagga ggaggcgttc cgcgcgacat
    13501 gccggtttcg caacgagctg gcgctcaaca cctgtttcgc ggcggcgcga cggcgttggg
    13561 acgaaggcgg cgacgcgctc gacatcatcg ccgagctcat caacatccgc tacggcgata
    13621 cgcggcggat ggccaacgcc tcgcagcatc tcgtcgagct gaacgccgag acgttcaagc
    13681 gctgcaacga catcatcacc gaggtcgccc tccatttcga agcggcgctg gcgaaattca
    13741 ttatcgagat ggaagagggc ggcctgctgc agcttcgcgc cgatgcgacg cctgagcaac
    13801 tcgcgcaagc gctggccaac ggcgcgcgcg gcgtcaatca acgcttgccg ccagcggcgc
    13861 cggacgcgct cgcctcgtgt tatcgcgaaa tctgctgctt cgtcctgttt ggcggcgccg
    13921 agattgcggc ggcgaaccag ccgccgcggc gccaggcccc caaacgggcg ggggacccgc
    13981 gttcttgagc ggcgaagcgc cgccgctcga tccggagagc gccgaggccg ccgcaggccg
    14041 cgcgatgatc gccgacgcgc tcgaccgcct gccgctgacg aagctccatg ttgcgatcct
    14101 ggcgctgtgc gcgatgggcc tcgccgccga tatcggcgaa gtcgcgctca gcaacacgtt
    14161 ctccgccctt ctcctcgcgc cgccttccaa cgccccgcgc ggcgccgtct ccttgcattt
    14221 agcggcggtc ttcgccggcg gcgcgatcgg cgcgcccctg ttcggctggc cggcggacaa
    14281 aagcggccgg cgcgcggtgg ccggcaagcc ggtcgactaa atcgccgccg cggcgcggcg
    14341 cgcgttgacc cggcgttaat gccgcccggc gatagtcccg tcattcatcg aaccggacgg
    14401 ggcggggcgc gacgtttatg ctgaaaagct tgaccatcaa ggcgcggatc gcgctgatca
    14461 gcgtcgtgtt cgtcgccgga ctcgcggtga tcggcggcgc caatctcgtc ggcagccgca
    14521 acgtcgatga ggctttcgcc gacgccaagg cctacgccgc cttgcaggtg aaggccggca
    14581 agatcgccga aactgcgatc ggcttgaaag gcgtcgcccg cgacgtgcgc taccgccacg
    14641 aatcggtcga gctcgcaaaa ttcgccgacg gcctcgcccg cctgaccgga cttgtcgacg
    14701 atctcgccgc cgcgccgcgc ggccaggcgg tggcccagca gatttccgat ctgaaaaacc
    14761 aaatcgccgc gctcgccaag caattcgcga ccgtgcaggg cttgcagaag ccgctcggcg
    14821 attccggatc ggccgggctc gccgaccgcg ccgattcgac cggcgcaagc ctcgtcgaca
    14881 tgctcaaggc caatatcgcc gagcaggatt cgctcgaagc cgagcggttg ctcgccgcga
    14941 cttttgcgat gcgccgcgcc caatcggaat acgcccggac gttcgacgat agcctgaccg
    15001 gcgagtggga ggtcggctat gggcgcttcg attcgacgct gaaaaacgcc gagattgacg
    15061 cggcgaccaa gcgcctgatc gcctcgacgt tcaaggacta tgcgaaatcc ttcaaggccg
    15121 cgagcgccgc ggaaaccgat tacatgcgcg ccgccgaacg ggtcagcggc ggcctcgact
    15181 tgatcgcccc ggtcttgcag gagcttgacg cgaaggcgtc gcaagacgcg gcggcggccg
    15241 gccagcgcct cgccgcctcg gaggccctga ccaagaagat catcttcgcc gctatggcgc
    15301 tcgcactggc cggcggcctg atcgccgcgg cgatcgtcgc gcgcaccacc gccaagccgc
    15361 tcggccagct ccgcgacgca atgctcggcc tcgcctcggg cgatcatgcg actgaggtgc
    15421 cggcgctcgg ccgcgccgac gagatcggcc aaatggccaa ggccgtgcag agcttcaagg
    15481 aggccgcttt ggaccgcgag cggcttgagc gcgacgcggc ggcgcaacgc gaagcgagcg
    15541 aagccgaacg ccgcggcgcc gaagccgagc gcgcggcgcg cgagagggat caacagcgcg
    15601 tcgtcgcggt catcgcgcat ggccacgagc aattggccaa cggcaacttg accttccgca
    15661 tcgacgagac gttcccgccc gcctatcaaa agatgaagga cgacttcaac gcggcgatcg
    15721 cgcaaatgca gcaggccatg tcggtgatct cggcgacgac gcaaggcatt cgcgccaatg
    15781 ccggcgaggt ctcgcaagcc tcggacgatc tctcgcgccg caccgagcag caggcggcga
    15841 gcctcgaaga gagcgccgcc gctttgagcg aaatcaccgc aacggtcaag aaagccgccg
    15901 agggcgccaa tctcgccagc cgcgcggtcg ccgccgccga cagcgacgcg cggaaaggcg
    15961 ccgaggtcgt gcgccatgcg gtcgccgcga tggacgcgat cgccaaatcg gcggcgaaga
    16021 tcaaccagat catcggcgtc atcgacgaga tcgccttcca gactaacctg ctggcgctga
    16081 acgccggcgt cgaggcggcg cgggccggcg ataccggccg cggcttcgcc gtcgtcgccg
    16141 ccgaagtccg cgccctcgcc cagcgctcgg ccgaggccgc caaggagatc aagggcctga
    16201 tctcggcctc gaccaaccag gtcaatcaag gcgtcaagct ggtcggcgaa tccggccaat
    16261 cgctcgaacg agtcatggcg caggtcgccg acctcaaccg gatcatcctc gaaatcgccg
    16321 agggcgcgcg cgcccaggcc gccggcctcg aagaggtcaa cgccgcgatc ggccagatgg
    16381 acaagatgac gcagcaaaac gcggcgatgg tcgaggagac caccgcgacc agccattcgc
    16441 tgtcgcagga gacttcggat ctgtcgagcc tgatcggcgc cttccaggtc ggcggggcgg
    16501 ccgaggaagc ccagttgcgg cacgaattgc agaacgccgc gccgcacgct ttctccgcgc
    16561 cgcgcaacgg cgccgtccgg ccgcggggga cgccgccggc cagcggcccg cggccgcgcg
    16621 gcccgacgct ggtccgctcg cattaacaaa ggcgcgaatc ttcgctggcc gcttcgattt
    16681 ccgcccgggc tcgcgcccat ctgatcgaga tttgcaattt tacagagcgg cgcggcggtc
    16741 ttgccagcgc cgcccttccc ccctatagac gcggcttcgc gcccgttccg acacccctgg
    16801 aggcggggcg cgtcaacttc aaaggatgat cgacatggcg gcgaccaaac agctcgcggc
    16861 cgtggcgcgc agcgggatcg gcaagggggc cgcccgttct gtccgccgcg caggccgcgt
    16921 tccagcagtg atttatggcg gcggcgaaac ggccgcgccg atcgcgctcg attacaagga
    16981 agtgaatcag ctcatcaacg ccggccattt cctcaccacg attttcgagc tcgacgtcga
    17041 gggccgcaag gagcgcgtcc tgccgcgcga ttaccagctc gacgtcgtca ccgaccggcc
    17101 gcttcatgtc gatttcctgc gcctgaaagc cggctcgcgc gtgcgcatcg cgacgccggt
    17161 ccatgtcgtc aaccaggaca aggcgcccgg catcaagcgc ggcggcgcct tgaacctggt
    17221 tctgcatgcg atcgagctgt gggcgccggc cgaggcgatc ccggattcga tcaccatcga
    17281 cctgaccggc ctcgacatcc acgattcggt ccatctttcg aatatccaac tgcccgaggg
    17341 ctgcaagccg gtcgatacgc gcaccgactt tacgatcgcg accgtggtgc cgccgaccgt
    17401 ctatgtcgaa gaagcgcccg tcgcagcggc cgccgcgacg accgccgccg cagcgcccgc
    17461 agccggcgca gccgccccag cgcccggcgc agcgcctgcg gctccggccg ccgcagcgcc
    17521 cgccgctcca gccaagggcg ccgcggcccc cgcggccaag aagtaacgct ggagccgcgg
    17581 tcgccgaccg cggctacgcc ctcctcgcgc gcggcgcatg atccttcttg caggcctcgg
    17641 caatcccggc gcgcgccacg ccaagaaccg ccacaatatc ggcttcatgg cgatcgaggc
    17701 gatcgccaag gcgcatcgca tcggcccgtt ccgcgcgcgc tttcagggcg cggccgcgga
    17761 aggcctgatc ggcggcgagc gcgcgctgct gctgatgccg cagacctaca tgaacgattc
    17821 cggccgcagc ctcggcgagg cgatgcgctt ccacaagctc gatcttgccg acatcgtcgt
    17881 catccacgac gagctcgacc tcgcgccagc caaggtgcgc gtgaaggtcg gcggcggcaa
    17941 tgccggccat aacggcctgc gctcgatcag cgcccatatc ggcaacgagt acaagcgcgt
    18001 ccgcctcggc atcggccatc ccggcgacaa agcgctggtg caccaatatg tgctcagcga
    18061 tttcttcaag gccgagacgg gctgggtcgc cgccgtctgc gacgccgtcg cccgcgccgc
    18121 cgacctgctg gcgcgcggcg aggacgccag cttccaaaac aaggtgcatc tcgccgtaaa
    18181 tgcggccggc ttcggcgaaa atgcgccggc cgaggactga cgctgcgctt ccgcctgacc
    18241 cgcgtcttgt aaaaacttca gcctgacgct tgcagcaata attttaacac gaagatcacg
    18301 aagccgggcg caaaggacaa aggcgcgtca gatttttgca aaagtagcca ggcagagagg
    18361 cgctcagccc gcgacaaacc gcgcccgttg cgacgattgg tatcgctctg ccggccgccc
    18421 tccgcttgtc gccgcgcccc gcttcatgct tctagaggcg caaccgcttg attccgtcga
    18481 ggccagccat gcccgcttcc gaccgcctgc tcgttctcgt ccgccacggc caaagcgact
    18541 ggaatctgaa aaaccttttc accggctggc gcgacgtcga tctctcgccg aaaggcgtcg
    18601 aggaggctcg ggaagcgggg cgccggctga agcgccaagg cctt
//
LOCUS       DATGJW010000506         9300 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1135668, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000506 DATGJW010000000
VERSION     DATGJW010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9300)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9300)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9300
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1135668"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(194..1588)
                     /gene="gor"
                     /locus_tag="VKU03_15305"
     CDS             complement(194..1588)
                     /gene="gor"
                     /locus_tag="VKU03_15305"
                     /EC_number="1.8.1.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011509994.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004362 - glutathione-disulfide reductase
                     (NADP) activity [Evidence IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutathione-disulfide reductase"
                     /protein_id="HLJ72685.1"
                     /translation="MTFDVDLFVIGAGSGGVRAARIAADYGARVMIAEEFRIGGTCVI
                     RGCVPKKLLVYASRFAEDFEDAAGYGWQVGETRFDWPSLVAAKEKEITRLSAAYRANL
                     ERARVAIVEERARLESPQAVRLASGRIVTAKHVLIATGAAPDYPLAIPGLEHAISSNE
                     IFDLATFPRRLLVAGGGYIGVEFASIFARLGAEVTLIARASNILRGFDEDMRDGLRDA
                     LTRAGVKFRFGCLPTQVERRGGARRVTLSDGETVIVDQFLNAYGRRPNTKDLGLDKAG
                     VALDALGAVKVNAFSQSNVESVYAVGDVTNRLNLTPVAIREGHAFADTVFGGKNVAMD
                     HRNVPSAVFATPEIGAVGLTEAQARLHHRIVDIYRANFTPMKATLSGRKERTIMKIVV
                     DGETDRVLGVHILGHEAGEMAQLLAIAVKMGATKADFDATVALHPTAAEELVTMRKRA
                     ARYERAEGESAYLP"
     gene            complement(1626..2132)
                     /locus_tag="VKU03_15310"
     CDS             complement(1626..2132)
                     /locus_tag="VKU03_15310"
                     /inference="COORDINATES: protein motif:HMM:NF021361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2059 domain-containing protein"
                     /protein_id="HLJ72686.1"
                     /translation="MNLRPPFAKPLAGALLLTALAFSPAYAADPSPAAIADARTIILT
                     SGIARSFDAVVPQMLGVLERNALATRPDIKDKLHATLLKIEPEFVKTEDQIINAAALS
                     LAKRMTEPDLKETAAFFQSPAGKKYVEAQPAALTDIVAALQGWQQKLSTDMMTRVREE
                     MKKQGIEF"
     gene            complement(2163..2867)
                     /gene="rpiA"
                     /locus_tag="VKU03_15315"
     CDS             complement(2163..2867)
                     /gene="rpiA"
                     /locus_tag="VKU03_15315"
                     /EC_number="5.3.1.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015666082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004751 - ribose-5-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0009052 - pentose-phosphate shunt,
                     non-oxidative branch [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-5-phosphate isomerase RpiA"
                     /protein_id="HLJ72687.1"
                     /translation="MPDVERLKREAARRALDLVRPGMRLGLGSGSTARHFIDLLGEKV
                     RQGLAVTGVPTSETTKRQAESLKVPLTSLDETPELDLAVDGADEIDPSLRLIKGGGGA
                     LLREKIVAAAAKRMIVIADQSKLVGRLGAFALPIEVVPFGLEATRRHIAQSAGQCGCR
                     GEIRLRLAAGGAPFVTDGGHYILDGAFGAIEDPARLAAALAAIPGVVEHGLFIGLAQG
                     AILAGAEGLKILGRID"
     gene            3036..3290
                     /locus_tag="VKU03_15320"
     CDS             3036..3290
                     /locus_tag="VKU03_15320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018897448.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapB family
                     antitoxin"
                     /protein_id="HLJ72688.1"
                     /translation="MPLYIRDREVDELAAKVQAAMKAPTKTEAVRLALEHELERQRAD
                     AAPLRSRLAKAIAMAEAMGVRKGSTKDFDMKAFTDEMWGC"
     gene            3290..3724
                     /locus_tag="VKU03_15325"
     CDS             3290..3724
                     /locus_tag="VKU03_15325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ72689.1"
                     /translation="MFLDASAIVSILAQEDDADLLIEQLEAAKTQRYVSPTTMFEAAM
                     ALARIAAKEAKTSVSRENIEEAQATLDEFVATVGAKEVEISGDIGRRAVEAAKLYGRL
                     VGHRAGLNFGDCFAYACAKAHRVPLLYKGNDFGHTGVNANPI"
     gene            complement(3755..4195)
                     /locus_tag="VKU03_15330"
     CDS             complement(3755..4195)
                     /locus_tag="VKU03_15330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266706.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72690.1"
                     /translation="MRLSKPMLVLAAAAAAASFSAPAPASEAAAYEFLAAPQINLSLV
                     YRLDKLTGEVIACQYGHNPGKTDIEPGAFGITVCYRSGEGAGKQEPGQYGLVATRHEQ
                     EGGVFRVDYRSGSLSICYLYFQKEKQGDREVITDQYVVCTPPAK"
     gene            4324..5028
                     /locus_tag="VKU03_15335"
     CDS             4324..5028
                     /locus_tag="VKU03_15335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918221.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAD-IA family hydrolase"
                     /protein_id="HLJ72691.1"
                     /translation="MDAKPPILVFDLDGTLADTAGDLIATLNAILTGESLPPVPLAHA
                     RKLIGAGAKALIQRGFAAEGRDLSPDKLDRLYAAFIQRYEDHVADETRLFPGVEAALD
                     RFAAAGYAFAVCTNKLERSSLLLLEALGVKSRFRAICGQDTFKIAKPDRRIFDATVDR
                     AGGDPATAIMIGDSATDIETARAARAPVVAVDFGYTDLPIENFGPDRVISHFDQLWDA
                     VAALRPAAGEANPPPP"
     gene            5074..5148
                     /locus_tag="VKU03_15340"
     tRNA            5074..5148
                     /locus_tag="VKU03_15340"
                     /product="tRNA-Val"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:5106..5108,aa:Val,seq:cac)
     gene            5239..7011
                     /locus_tag="VKU03_15345"
     CDS             5239..7011
                     /locus_tag="VKU03_15345"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018068496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ72692.1"
                     /translation="MSETRKLVAILVADVVGFSRLAGADEDRILARLRTLRSDLIDPT
                     VAVHHGRVVKRTGDGAVVEFRSVVDAVRCAIEVQNGMAERNAGLPEDRRIEFRVGIHL
                     GDVVEEDDGDLMGDGVNIAARLEGVAKPGAICLSEDAYRQVKARLDLKVSDLGPTPLK
                     NIAEPVRVYSVEVGILSQAPPKSATSEAPSRQLALPERPSIAVLPFQNMSGDPDHEYF
                     VDGMVEDIITGLSRIKWLFVIARNSSFVYKGNSVDVRQVGRDLGVRYILEGSVRRAGN
                     RLRVTGQVVEAETGRHVWAERYDRTLEDIFALQDELTTSVVAAIEPSLRQAEVERVKR
                     KRPENLDAYDLLLRALPDIYPAMPDGAAKALPLLKSALDLEPDYATAHGFAAWCNEIL
                     FIRGGAREENRLDGIRHAHAAIQYGRDDATALALGGFVMGMVGHDREAARRAFDAALT
                     LSPSCGAAFLFGSVIMATGGDAELGVEWGERAVRLSPLDPGNYGPYFAIALGKVQRGD
                     FAAAAEAARKCFQANPNFSYAHMLLAATHAQLGRTNEAGLAAKRVLELEPGYSISGMC
                     AAVGLHPSIAEPLSEALRKAGLPG"
     gene            7102..8811
                     /locus_tag="VKU03_15350"
     CDS             7102..8811
                     /locus_tag="VKU03_15350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72693.1"
                     /translation="MAVNLEKAAVPAPQWAALRLWLLAPALGLAFVGLAAWLQRGFWL
                     NSCPGQIGADCDGRWFDLHRVFFAETILCLAACAVAAFAVHSVGMNLSGQAVHREKQA
                     RRLAVFACALALAGPLLTAWFVLHRFANSGDEYSYIFQARTFAGLRLWENPPALGVDL
                     IPNRTFIFDGKWISQYPPGWAFVLAAGSRLGLPPWTINPLIGAASVAALALFCRRVAA
                     SWTSALAVLLYAATPFYVLNAASYFPHVFSSLLVLLLCLCLQGDEGGKTRLLAAGACL
                     GALAATRYFDVLALIPALSMWLRTAKPADWPRRIGLLAAGFAPFVALLGLYQAVLLGS
                     PFRSTYSVINSPDTFLSLDPSLLAISPLVAAVRLGELALWTSPLLLLAYFASLAAKWK
                     TRSFAFYDLIFPSFVIAYLAFANFGGNRYGPRYYLDAFPLAVATIASASPSLAARAPR
                     AFARLAPIGVAVFVLYLVAVWPLAAAGFSRQIWARQEPFRLAAEAGLANAIVIEDTTG
                     GPGLVLPDLVRNPPSMDAPVLYARAGADVGAIREAFPERSIWRYSRADPAKPGRLIRL
                     PPP"
BASE COUNT         1470 a         3062 c         3040 g         1728 t
ORIGIN      
        1 cgatttgatc cgacgggtca aatgatggcg ctttcctccc ccgcgaagcg ggggaggggg
       61 accatgcgaa gcatggtgga ggggtacggc ctctcaggat aacgcccaaa cgccgcagcc
      121 ccctccaccg cgctgacgcg cggtccccct cccccgttcg cttcgcttca cgggggagga
      181 tccggacgac cgtttagggg aggtacgcgc tctcgccctc cgcacgctca tagcgcgcgg
      241 cgcgcttgcg catcgtgacc agctcttcgg cggcggtcgg gtggagcgcg accgtggcgt
      301 cgaaatcggc cttggtcgcg cccatcttga ccgcgatagc gagaagctgc gccatctcgc
      361 cggcctcgtg gccgaggata tggacgccga gaacccggtc tgtctcgccg tcgacgacga
      421 ttttcatgat cgtgcgctcc ttgcggcccg acagcgtcgc cttcatcggt gtgaaattcg
      481 cccgataaat gtcgacgatc cggtgatgca ggcgggcctg cgcctcggtg aggccgaccg
      541 cgccgatttc cggcgtcgca aaaaccgcgc tcggcacgtt gcggtggtcc atcgcgacgt
      601 tcttgccgcc gaacaccgtg tcggcgaaag catggccctc gcgaatcgcg accggcgtca
      661 ggttcagtcg gttggtcacg tcgcccaccg catagacgct ctcgacattg ctctgtgaaa
      721 acgcgttcac cttgaccgcg ccgagcgcgt ccagcgcgac gccggctttg tcgaggccga
      781 ggtccttggt gttcggccgc cggccatagg cgttgaggaa ctgatcgacg atgacggtct
      841 cgccgtcgga aagcgtcacg cgtcgcgccc cgccgcgcct ttcgacctgc gtcggcaggc
      901 agccgaagcg gaatttcacg ccggctcggg tcagcgcgtc gcgcaggccg tcgcgcatat
      961 cctcgtcgaa gccgcgtagg atgttgctgg cgcgggcgat cagagtcacc tcggcgccca
     1021 ggcgcgcgaa gatcgaggcg aattcaacgc cgatatagcc gccgccggcg acgagcaggc
     1081 ggcgcggaaa ggtcgcgagg tcgaaaatct cgttcgagct gatcgcgtgt tcgaggccgg
     1141 gaatggcgag cggataatcg ggcgccgcgc cggtcgcgat caggacgtgc ttagccgtga
     1201 cgatgcgccc gctcgccagc cgcaccgcct gtgggctttc gagccgcgcc cgttcttcga
     1261 cgatcgcgac gcgggcgcgc tccagattgg cgcgataggc ggcggagagg cgggtgatct
     1321 ccttctcctt ggcggcgacc aggctcggcc aatcgaaccg cgtctcgccg acttgccagc
     1381 catagcctgc cgcgtcctcg aagtcctcgg cgaagcggct ggcgtaaacc agcagctttt
     1441 tcggcacgca gccgcggatc acgcaggtgc cgccgatccg gaactcctcg gcgatcatga
     1501 ctcgcgcgcc gtaatcggcg gcgatccgcg cggcgcggac gccgccggag cctgcgccaa
     1561 tcacgaaaag atcgacgtcg aacgtcatcg cgcctcgaag tacgacgctc gcgaaacgag
     1621 cgcgctcaaa attcgattcc ctgcttcttc atctcctcgc gcacccgcgt catcatgtcg
     1681 gtcgacagtt tctgctgcca gccttgcaag gcggcgacga tgtcggtgag cgcggcgggc
     1741 tgggcttcga catatttctt gccggccggg ctctggaaga aggccgccgt ctccttcagg
     1801 tcgggttcgg tcatgcgctt ggcgaggctg agcgccgcgg cgttgatgat ctgatcctcg
     1861 gtcttgacga attccggctc gattttcagc aacgtcgcgt ggagcttgtc cttgatgtcc
     1921 ggccttgtcg ccagggcgtt gcgctccagg acgccgagca tttgcggcac gaccgcgtcg
     1981 aaggagcggg cgattcccga ggtcaggatg atcgtccgcg cgtcggcgat cgcggccggc
     2041 gacggatcgg cggcgtaggc cgggctgaag gcgagagcgg tcagaagcag cgcgcccgct
     2101 aagggcttgg cgaaaggcgg tcgaaggttc acgtcaaaca tctcctttgg atcggttcaa
     2161 gatcagtcga ttcggccaag gattttcaag ccctccgccc cggcgaggat cgcgccttgc
     2221 gcaaggccga tgaaaagccc gtgctcgacc acgcccggaa tcgccgccaa agcggccgcc
     2281 agccgggccg ggtcttcgat ggccccgaaa gcgccgtcga gaatgtaatg gccgccatcg
     2341 gtgacgaagg gcgcgccccc ggcggcgagc ctcagccgaa tttcgccgcg gcatccgcat
     2401 tggccggcgg attgggcgat atgacggcgg gtcgcctcca ggccgaacgg aacgacctcg
     2461 atgggcaggg cgaacgcccc gagtcggccg accagcttcg attggtcggc gatgacgatc
     2521 atccttttgg ccgccgcggc gacgattttc tcgcgcagca gcgccccgcc gccgcctttg
     2581 atgagcctta gcgaggggtc gatctcgtcg gcgccgtcga cggcgaggtc gagctccggc
     2641 gtctcgtcga gggaggttaa gggcacttta aggctttcag cctgacgctt cgtcgtttcc
     2701 gaagtcggca cgccggtcac ggcgaggccc tggcgcacct tttcgccgag cagatcgatg
     2761 aaatgacgcg ccgtcgagcc gctgccgagc ccgagccgca tgcccggccg gacgaggtca
     2821 agcgcgcgac gggcggcttc gcgcttcagc cgttcgacgt ctggcaaggg gcggttccgg
     2881 gtgaggcggc ccatcgggcg cgcgttcgcg gcatggccct aacgccttaa gctttgaaac
     2941 gcaaatggaa gcgcggccca tgcttatagg gctcgaccca ttcaaacgcc gtgtatatcc
     3001 ttgctgaata ttcagcggcc gaaattcgag gatccatgcc cctctatatc cgtgatcggg
     3061 aagtcgacga gttggccgcg aaagtgcagg ccgccatgaa ggcgcccacg aaaaccgaag
     3121 cggtcaggct cgccctggag catgagcttg agcgccagcg cgcggacgcc gcgcctctgc
     3181 gctcccgtct cgccaaggcg atcgccatgg ccgaggctat gggggttcgc aagggctcca
     3241 ccaaagactt cgatatgaaa gcttttactg acgaaatgtg gggctgctga tgtttctcga
     3301 cgcgtcagcc attgtcagca tcctcgcgca ggaagacgac gccgatctgc tgatcgaaca
     3361 gctcgaagcc gcgaagaccc agcgctatgt ttcgccgacg acgatgttcg aagcggcgat
     3421 ggctctggcg cgcattgcgg ctaaggaggc gaaaacctcg gttagccgcg agaatattga
     3481 agaggcgcaa gcgacactcg atgaatttgt cgcgacggtc ggcgccaagg aagtcgaaat
     3541 ttccggcgat atcggtcgca gggccgttga agcggccaag ctgtacggtc ggctcgttgg
     3601 tcatcgggca ggtctgaatt tcggcgattg tttcgcttac gcgtgcgcga aagctcatcg
     3661 cgttccgctg ctgtacaaag gcaacgattt tgggcatacc ggcgtaaacg ccaacccgat
     3721 ttagtccgct gctttcgcgg cgcttaaggc gcttctactt cgccggcggc gtgcagacca
     3781 cgtattgatc ggtgatgacc tcgcggtcgc cctgcttctc cttctggaaa tagagatagc
     3841 agatgctgag cgagcctgag cggtagtcga cgcggaagac gccgccttcc tgctcgtggc
     3901 gggtcgcgac gaggccgtat tggccgggct cctgcttgcc ggcgccctcg ccggagcgat
     3961 agcagacggt gatgccgaac gcgccgggct cgatgtcggt cttgccggga ttgtggccgt
     4021 attggcaggc gatcacttcg ccggtcaatt tgtcgagccg gtagacgagg ctcagattga
     4081 tctgcggcgc ggcgaggaac tcgtaggccg cagcctcgct cgccggcgcg ggcgcgctga
     4141 aagacgcggc ggccgccgcg gccgcgagga cgagcatcgg tttcgataag cgcatgaaag
     4201 ccccgttttg cgcgaaagga cgctcggcga gagcgccgaa tcagcttcaa acgcattgta
     4261 ctcgccgtgg cggccgaaac ccatggcttg ccggccggcg cgccggcgct tatgcgtccg
     4321 gccatggacg ccaagcctcc catcctcgtc ttcgatctcg acggcacgct cgccgacacg
     4381 gccggcgatc tcatcgccac gctcaacgcc attctgaccg gcgaaagctt gccgccggtt
     4441 ccgctcgccc atgcgcgcaa attgatcggc gcgggggcga aagcgctgat ccagcgcggc
     4501 ttcgccgcgg aagggcgcga tttgtcgccc gacaagctcg accggctcta cgcggccttc
     4561 atccagcgct atgaagatca tgtcgccgac gagacccggc tcttcccggg cgtcgaggcg
     4621 gcgctcgacc ggttcgccgc ggcgggctac gccttcgccg tctgcaccaa caagctcgag
     4681 cgctcgtccc tccttcttct cgaagcgctc ggcgtcaaaa gccggttccg ggcgatttgc
     4741 ggccaggaca cgttcaagat cgccaagccg gaccggcgca ttttcgacgc gaccgtcgat
     4801 cgagccggcg gcgatccggc gacggcgatc atgatcggcg attcggcgac cgacatcgag
     4861 acggcgcggg ccgcccgcgc gccggtcgtc gccgtcgatt tcggctatac cgacctgccg
     4921 atcgagaatt tcggccccga ccgcgtcatc tcgcatttcg accaattgtg ggacgccgtc
     4981 gccgccttgc ggccagccgc aggtgaagcg aacccgccgc cgccttgact cgccggcgag
     5041 cgccgcccta aaccgtcggc cgtccggcct tttgggcgcg tagctcagcg ggagagcact
     5101 cccttcacac gggaggggtc acaggttcaa tccctgtcgc gcccaccagg acaatctcca
     5161 ttttgcgatg ttccgtcgcg gcagacgctc ttaatccgcc gattgtgcta tcttgccctt
     5221 gggcaggggg cacgcgctat gagcgaaacc cgcaagctgg tcgccattct ggtcgccgac
     5281 gtcgtcggct tctcgcggct ggccggcgct gacgaggatc gcattctcgc ccggctgaga
     5341 accctcagga gcgacctgat cgacccgacc gtcgccgttc atcacggccg ggtcgtcaag
     5401 cgcaccggcg acggcgccgt cgtcgaattt cgcagcgtcg tcgacgccgt tcgctgcgcg
     5461 atcgaggttc agaacggcat ggccgagcgc aacgccggcc tgccggagga ccgccgcatc
     5521 gagttccgcg tcggcattca tctcggcgac gtggtcgagg aagatgacgg cgacctgatg
     5581 ggcgacggcg tcaacatcgc cgcgcggctc gaaggcgtcg ccaagcccgg cgcgatctgc
     5641 ctctcggaag acgcctatcg gcaggtgaag gcgcggctcg atctcaaggt cagcgatctc
     5701 ggcccgacgc cgctgaagaa catcgccgag ccggtgcgcg tctattccgt cgaagtcggc
     5761 atattgtcgc aagcgccgcc gaaatcggcg acgtcggagg cgccttcccg tcagctcgcc
     5821 ttgcccgagc ggccgtccat cgccgtcttg ccgttccaga acatgagcgg cgaccccgac
     5881 catgaatatt tcgtcgacgg aatggttgag gacatcatca ccggcctgtc gcggatcaag
     5941 tggctcttcg tgatcgcgcg caattcaagc ttcgtttaca aaggcaactc ggtcgatgtg
     6001 cgccaagtcg gccgcgatct cggcgtccgc tacatccttg aagggagcgt acgaagagcc
     6061 gggaaccgct tgcgcgtcac cgggcaggtc gtcgaggcgg agaccggccg ccacgtctgg
     6121 gccgagcgct acgaccgcac gctcgaagac atctttgcgc ttcaggacga attgacaacg
     6181 agtgtggtgg cggcgatcga gccgagcctg cgtcaagcgg aggtcgagcg cgtcaagcgc
     6241 aagcgacccg aaaatctcga cgcttatgac ctcttgctgc gcgccttgcc cgatatttac
     6301 ccggctatgc cggacggtgc ggcgaaggcg ctgccgctgc tcaaaagcgc gctcgatctg
     6361 gagcccgact acgcgaccgc gcatggattt gccgcatggt gcaacgaaat cctcttcatt
     6421 cgcggcggcg cgcgggagga gaaccggctt gacggaatcc gccatgcgca cgcggcgatc
     6481 caatacgggc gggacgatgc gaccgctttg gcgcttggcg gcttcgtgat ggggatggtc
     6541 gggcatgacc gggaggcggc gcggcgcgcc tttgacgcgg ccttgacgct aagtccgtct
     6601 tgcggcgccg ccttcctttt cggcagcgtc atcatggcga cgggcggcga tgcggagttg
     6661 ggcgtcgaat ggggcgagcg cgccgtgcgc ttgagccctt tggacccagg aaattacgga
     6721 ccttattttg cgatcgcatt gggcaaggtt cagcgcggcg acttcgcggc ggcggccgag
     6781 gccgcgcgca aatgctttca ggcgaatccg aacttcagct acgcgcatat gctgctcgcg
     6841 gcgacgcacg cgcagctcgg cagaacgaac gaggccggct tggcggcgaa gcgggtctta
     6901 gagctcgagc ccggctattc gatcagcggc atgtgcgccg ccgtgggcct gcatccatcg
     6961 atcgccgagc ccttgtccga ggcgctgcgc aaggctgggt tgccgggata agcgcttgcg
     7021 cgcctcgcgc tgacctttgc gaattcttca ataagaagcg gcgacaatcg acccggctcc
     7081 gtcggtgagc cgaggatgga tatggccgtc aatttggaaa aagcagccgt gccggcgccg
     7141 caatgggcgg cgctgcggct ctggctgctt gcgccggctt tgggcctggc gtttgtcggc
     7201 cttgccgcct ggctgcagcg gggcttctgg ctgaattcat gtcccgggca gatcggcgcc
     7261 gattgcgacg gccggtggtt cgacctgcat agggtgttct tcgccgagac catcctctgc
     7321 ctcgccgcct gcgctgtcgc cgccttcgcc gtccacagcg tggggatgaa tctgtccggt
     7381 caagccgttc atcgtgaaaa gcaggcgagg cggctggccg tcttcgcctg cgcgctcgcc
     7441 ttggccggac cgctcctgac ggcttggttc gtgctgcatc gattcgccaa ttccggcgac
     7501 gaatacagct atattttcca ggcccgtact ttcgccggct tgcgtctttg ggagaatccg
     7561 ccggctttag gcgtcgacct gatccccaat cgcaccttca tttttgacgg caaatggatc
     7621 agccaatatc cgccgggatg ggcatttgtt ctggccgccg ggtcgcggct cggcctgccg
     7681 ccctggacga tcaacccgct tatcggcgcg gcgagcgtcg cggccttggc gctattctgt
     7741 cgccgcgtcg ccgcttcctg gaccagcgcg cttgccgttc tgctctatgc ggcgacgccg
     7801 ttctatgttc tgaacgccgc ctcgtatttc ccgcatgtct tctcgtcatt gcttgtcctt
     7861 ctcctctgcc tctgcttgca gggcgacgaa ggggggaaaa cgcgtttgct cgcggcggga
     7921 gcgtgcctcg gcgcgctggc cgcgacgcgc tatttcgacg ttctggcgct gatcccagcg
     7981 ctttcgatgt ggctaaggac cgccaaacct gccgattggc cgcgccgcat cggtttgttg
     8041 gccgcgggat ttgcgccgtt cgtcgcgctg ctagggctgt accaggccgt cctgctcggc
     8101 agcccgttcc gcagcaccta ttccgtgatc aattcgcccg acacgtttct ctcgctcgat
     8161 ccgtcgctcc tcgcgatcag tccgctggtc gccgccgtgc ggctcggcga attggcgttg
     8221 tggacgtcgc cgctcctttt gctcgcctat ttcgcgagcc tcgccgcgaa atggaaaacc
     8281 cggagcttcg ccttctacga tctgattttt ccatccttcg tcatcgccta cctcgccttc
     8341 gccaatttcg gcggcaaccg ctacggcccg cgttactacc tggacgcgtt tccgctggcg
     8401 gtagcgacga tcgcttccgc ctcgccctcg ctcgcggcga gggcgccgcg cgctttcgcg
     8461 cggctggcgc cgatcggagt cgcagtcttc gtcctctatc tggtcgccgt ctggcctctg
     8521 gccgccgcgg gcttctcgcg gcaaatctgg gcgcggcagg agccgttccg gctggccgcc
     8581 gaggccggcc tcgccaacgc aatcgtcatt gaggacacga ccggcggccc cggcctggtc
     8641 ctgcccgacc tcgtccgcaa ccctccgtcc atggacgcgc cggtcctcta tgcgcgcgcc
     8701 ggcgcggacg tgggagcgat ccgcgaagcc ttccccgagc gctcgatctg gcgctacagc
     8761 cgcgcggacc cggccaagcc cggccgcctg atccgccttc ctcccccgtg aagcgaagcg
     8821 aaacggggga ggggggacca tgcgaagcat ggtggagggg gtcgccggcg attgaggatt
     8881 atcccgagag accgggcccc ctccaccgcg ctgacgcgcg gtccccctcc ccgtctttcg
     8941 acgggggagg ggcgccactg tggcgccggc gccacttggg cgccttaaac ggcgcctcgc
     9001 gagagcgtga acgccttacg ccattgccag ctaagccttg cgccttctta atcgccgcgg
     9061 gttggagaca atgagtcgcg ggctgttgaa gcgaccattt cgaaactttt tccggctagc
     9121 ctcgtttgaa ggctaatcgc cggcgattgc attgattttg acggtaaagg ggtccttcca
     9181 tgcgccgtat ccctctgctt gttgcaggcg tcctggctgg cgttttcgcc gcctcctcgg
     9241 ccgcgcaagc gatgcaggac atcagcatct tcctctatcc tccaggtcag gcgagcagcg
//
LOCUS       DATGJW010000507         3566 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1136188, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000507 DATGJW010000000
VERSION     DATGJW010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3566)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3566)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3566
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136188"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(300..509)
                     /locus_tag="VKU03_15355"
     CDS             complement(300..509)
                     /locus_tag="VKU03_15355"
                     /inference="COORDINATES: protein motif:HMM:NF022603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2934 domain-containing protein"
                     /protein_id="HLJ72694.1"
                     /translation="MPDQEDDEEERIRAFAYRIWEEEGRPLGEADRHWEMARKAIEAE
                     DAERRRIAVGIDGPGKDAEKPEPGA"
     gene            complement(735..1744)
                     /locus_tag="VKU03_15360"
                     /pseudo
     CDS             complement(735..1744)
                     /locus_tag="VKU03_15360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009029029.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
     gene            complement(1867..2562)
                     /locus_tag="VKU03_15365"
     CDS             complement(1867..2562)
                     /locus_tag="VKU03_15365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017964691.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MgtC/SapB family protein"
                     /protein_id="HLJ72695.1"
                     /translation="MSLTLSWGDIALRIALTTLAGAAIGFDRGERGHAAGLRTTILVA
                     LAACLAMIEANLLMTTAGKAPDSFVVLDLMRLPLGILSGVGFIGAGAIFKRDNLILGV
                     TTAATLWFVTVIGLCFGGGQIGLGAAGSVLGIVIISGLKLIERQMKQHRNVEFAVKWR
                     GDDFGQDEALRLMAEAELEVMHMTVKYGADGEASEIRCALRERCRPDRHDLPKPLAEL
                     ARRANVVDLEWKT"
     gene            complement(2635..2865)
                     /locus_tag="VKU03_15370"
     CDS             complement(2635..2865)
                     /locus_tag="VKU03_15370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72696.1"
                     /translation="MNAYIVRARTEHRSAPLQHAFAISIAVCDTPEEAVSAAQKAFPE
                     WSDFAVIGRAPDSVIERRHLSAGNVERIAWRA"
     gene            3180..3467
                     /locus_tag="VKU03_15375"
     CDS             3180..3467
                     /locus_tag="VKU03_15375"
                     /inference="COORDINATES: protein motif:HMM:NF019452.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aa3-type cytochrome c oxidase subunit IV"
                     /protein_id="HLJ72697.1"
                     /translation="MRIERIDNPPADEDFAEHQRTFAFFLRLAAIAVLHIAACLIAVA
                     VGGLYGRWYLALFIFVIATIAAAMGVASENLAWKPGIAALAVSLLTFAVTA"
BASE COUNT          578 a         1192 c         1173 g          623 t
ORIGIN      
        1 cgagcgcttc gaccgcggcg ccttcgtcag gagtaaaaaa aatccacccg cccggcctga
       61 acggctcggg cggcgttccg gcatagggcc gccacggcaa gccgccggcc agcacgtccg
      121 cctgcgccac caggcgcccg ccgagaaaga aagcggcaga tgcaagcagt tctcgccggc
      181 gaaagcgcac cacgcctcac tcccgttggc gacaaaaacc atctgggagg cgtgttgttc
      241 cggcgcttat cccgatgaat gcgatcatcc tcggcgagcg acgggaaagc ttttccggct
      301 caggcgccgg gctccggctt ctccgcgtcc ttacccgggc cgtcgattcc gacggcgatg
      361 cggcggcgtt cggcgtcttc ggcctcgatc gctttgcgcg ccatctccca atggcggtcc
      421 gcctcgccga gcgggcggcc ctcctcctcc cagatgcggt aggcgaaggc tcggatacgc
      481 tcttcctcgt cgtcttcctg atcgggcatg agagtggtcc tccgttcgtc aacgcgcggg
      541 ccctcgaatt ggttcccggc ggctcatgcc cggccttgtg tcgggcgtcg acgcggcggc
      601 gccgcgtgga tggcaaggac aagcccggcc atgacgcgct gcggctgtac aaaattgacg
      661 cgctcgcccg ttcgtccgta cttcaaattt cctccgaagc gcggccgcgc gcgaaggtac
      721 gccggccggc gttctcaggg gccgccgcgg ccgccggtcg cgccatagac ctggccggtc
      781 gaatagctga gatcggacgc agcgagcgcg acatagatcg gggccagctc cgccggctgg
      841 ccgggccggc ccatcggcgt attggcgccg aattccggca gcttgtcggg cggctgtccg
      901 ccgctcggct ggagcggcgt ccaaaccggt cccggcgcga ccgcattgac gcggatgccc
      961 tttttcgcga tttgcttggc gagacctttg acgaagatca tgatcgcgcc cttggtcgcg
     1021 gcgtagtcga tgatgttctc gctcggctca taggcgttca ccgagctcgt gcagatgatc
     1081 gcggagccag gtttcaggtg cggcatcgcg gctttgatga tccagaacat cgcgtagaca
     1141 ttggtcttga aagtcgtatc gaattgctcg gtcgaaatgt cctcgatcga gtcgaccgcg
     1201 tgctgatgcg cggcgttgct gaccacaatg tcgagaccgc cgagctcgcg cgccgcgtcg
     1261 gcgaccagct tgcggcagaa ggcttcgtcg cgaatgtcgc cgggcagggg gaccgccttg
     1321 cggccggcct cgcggatgag ttgcacgacc tcgctggcgt cgggctcctc gaccgggaga
     1381 taaccgatcg ccacatccgc gccttcgcgg gcataggcga tcgcagccgc ccggcccatg
     1441 ccggaatcgc cgccggtgat cagagccttg cggccgagca ggcggccgct gcctttgtag
     1501 ctcgtttcgc catggtcggg ccgcgggatc atcttgaggg ccaggcccgg cggctcttgc
     1561 tgctggcgcg ggaaaggcgg gcgtggatac tcgtcgacgg gatttttcag ctctcatcgg
     1621 ccggacccgc ggcgccggcc ggcgccgcct gctgcgcgac cgccggcgcg ccggccatcg
     1681 ccaccgctcc ggcggcgccg gcaaggagcg cgcgtcgcga agcgtccatg ttctcgaagg
     1741 ccatggcttc tcctttgaag attgagcagc ggcgtcggca atgagccgtc tcgctctcgt
     1801 ggaccgagcg cgggcgccgc atcggcggtc aacccgagcc gcgcgcgcga gttccggtcg
     1861 tccgcgctaa gttttccatt ccagatcgac gacgttcgct cgccgcgcca attcggccag
     1921 aggcttggga agatcgtggc ggtcggggcg gcagcgctcg cgcaaggcgc agcggatttc
     1981 ggacgcctcg ccgtccgcgc cgtatttcac cgtcatatgc atgacttcga gctcggcctc
     2041 cgccatcagc cgcagcgcct cgtcttggcc gaaatcgtcc ccgcgccatt tgacggcaaa
     2101 ctcgacattg cggtgctgct tcatctggcg ctcgatgagc ttcagtccgg atatgatgac
     2161 gatgcctaat acggagcctg ccgctccgag cccgatttgg ccgccgccga aacaaaggcc
     2221 gatcacggtg acgaaccaca aagtcgccgc cgtggtgacg ccgaggatga gattgtcgcg
     2281 cttgaaaatt gcgccggccc cgatgaagcc gacgcccgac agaatgccga gcggcaggcg
     2341 catcaggtcg agcaccacga aggaatccgg cgccttgccg gcggtggtca tcagcaaatt
     2401 ggcctcgatc atcgccaggc aggcggcgag cgcgacgagt atcgtggtgc gcaggcccgc
     2461 cgcatggcct cgctcgccac ggtcgaagcc gatggcggct cccgcgagcg tcgtcagcgc
     2521 gatgcgtaac gctatgtccc cccagctcaa tgtcagcgac attgggcgcg ctccacgcgg
     2581 ctctccaaaa cggcgcgacc tcgcgtggtc ttcgcggccg gttgcaaggc gccgttacgc
     2641 ccgccacgcg atgcgttcga cgttccccgc cgatagatgg cgtcgctcga tcacgctatc
     2701 gggcgcgcgg ccgatcaccg cgaaatcgct ccactcggga aaagccttct gcgcggccga
     2761 caccgcttct tcgggcgtgt cgcagaccgc gatgctgatc gcaaaagcgt gctggagagg
     2821 ggcggacctg tgctcggttc gcgcgcggac aatataagcg ttcatcaggt ctcccgcccg
     2881 cgaggcttcg gcgtcttccc catgaatggt ctcgcctcgc cggaatttct gagagcaaag
     2941 ccgccgtcgc cgcgggcgca tgaaagaaag ccggcgactc cgcgaaccac cgcgacgttg
     3001 atcgtgtttt ccacgccagc cgtcacagca acccctcagg aacgactaaa gttccaaaaa
     3061 aggcgcaggt tgcggctcga atcatcgctg cgcgcgcctt cggcggccgg ttgcgcggga
     3121 aacaaaccgg tctttgaagc gttgggcgag caagggcttg cgctcgatga aggccagtta
     3181 tgaggatcga gagaatcgat aacccgcccg ccgacgagga tttcgccgag catcaacgga
     3241 ccttcgcgtt tttcctgcgt ttggcggcga ttgcggtgct gcatatcgcc gcctgcttga
     3301 tcgcggtcgc ggtcggcgga ctatacgggc gctggtacct ggccctcttc atcttcgtga
     3361 tcgccacgat cgccgcggcc atgggcgtcg ccagcgaaaa tctcgcctgg aagcccggaa
     3421 tcgcggcctt ggcggtgagc cttctgacct tcgcggtgac ggcatgagcg ggaaagcgcc
     3481 aattcccggc gtcgccgccg gggaaagagc ccaagccgag ccgatggaat ggacgctgac
     3541 gatcgcgccg gagaaagtct gagatc
//
LOCUS       DATGJW010000508         6003 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1136393, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000508 DATGJW010000000
VERSION     DATGJW010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6003)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6003)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6003
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1136393"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..795)
                     /locus_tag="VKU03_15380"
     CDS             complement(<1..795)
                     /locus_tag="VKU03_15380"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLJ72698.1"
                     /translation="MMVDELLTFKQTRDALAGGVSAVDIAKRTLDAIKRDNGVVNAFV
                     ALDEKRALIDAEHVDALIAAGVAPGPLAGMPIAVKDIIDVAGLPTKAGSPTRADAAPA
                     AADAPVVARLRAAGAIIVGKTHTVEYAFGGWGSNETTGAPLNPRDRGHPRTPGGSSSG
                     SGVAVAAGLVPAALGSDTGGSVRLPASFCGCVGLKTTIGLVETAGVVPLASMFDTIGP
                     LTNNVADAALMLAVMAPAQDRRSPGWSDRLAAIAQGAAGQAEGLRVA"
     gene            complement(856..1515)
                     /locus_tag="VKU03_15385"
     CDS             complement(856..1515)
                     /locus_tag="VKU03_15385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72699.1"
                     /translation="MPPPLRVAAILLGLSLLPLPLAGAYRLWRALHPAAVSGPVHATI
                     GGSTFVLPGGYFRPGSRGGGHLEKLSLAAFYPDFAPAGDLQDIASATDLAERHDRLVF
                     LTISEADSALDPADRPVKLYARFLDSNEWTQPGGLIARGFLPGSPFEGEELYFAAPEG
                     REFAARCGKPDQHRATPNFCIDDFRLGELNVELRFSANLLFGWEKLLAGARGLIEQAK
                     R"
     gene            1760..2272
                     /locus_tag="VKU03_15390"
     CDS             1760..2272
                     /locus_tag="VKU03_15390"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012336450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding protein"
                     /protein_id="HLJ72700.1"
                     /translation="MRNAVKLEETTRRSDRIAEISPVYLEALGMVERLHRRLLDVIKD
                     EFDRRGRSDVNAVQALLLYNIGDKELTAGELRTRGYYLGSNVSYNVKKMVEMGYLHHA
                     RSRIDRRSVRISLTEKGQEVHQIVAGLYEKHARTVEQIGGVSVDDFLRMNQSLARLER
                     FWTDQIKYRL"
     gene            complement(2348..3019)
                     /locus_tag="VKU03_15395"
     CDS             complement(2348..3019)
                     /locus_tag="VKU03_15395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020808183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysE family translocator"
                     /protein_id="HLJ72701.1"
                     /translation="MEIVVPSVVIVIPSAANLGLFAGAALVLLLIPGPAVLYIVARSA
                     EQGRLAGLVSDLGIHAATLVHALAAAVGLSALLASSALAFSAVKYAGAAYLVWLGLKK
                     IFGRDDGADAAMNLKRYSYRRLFRDGFIVNLLNPKTALFFFAFLPQFAEVGRGHVATQ
                     IVFLGLAFAGLGFITDGCYALIASAAGGWLKRGVFYRRFERYVSGALLVGLGVTAAIA
                     GNQRK"
     gene            3298..4581
                     /locus_tag="VKU03_15400"
     CDS             3298..4581
                     /locus_tag="VKU03_15400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLJ72702.1"
                     /translation="MKDVQLPRVCRRAFLSAAATFALTAFAGSAFAQQKFAAQAEWRQ
                     NYESSARLTVPRESTPVLSPETLAATEAAIQQYQGIVEHGGWTTVPTGQVLKLGAKSK
                     AVQALRQRLVVSGDLDSVAGDSPVFDSFVEAAVKRFQVRHGISPTGIVNDETFTELNV
                     PAETRLRQLQINVVRLRAFSGNLGERFVMVNIPAAAVETVENNTVYSHHVAGVGKIDR
                     QSPIMQAKAVEINFNPFWTVPASLIKKDLIPKMQADPNYLTEEKIRVFNKEGQEVSPS
                     SINWNSLDATQYKYRQDTGADINSLGSVRINIPNPYGVYMHDTPSKGIFGDDFRFVSS
                     GCVRVQDVRDYVAWLLKDTPGWSRDQIDQVIDSGQRLDVRLDKPVNVYWVYITAWATP
                     DGVVQFRPDVYQRDGAGPGPLATAVPEKPLATVQE"
     gene            4796..5656
                     /gene="kdsA"
                     /locus_tag="VKU03_15405"
     CDS             4796..5656
                     /gene="kdsA"
                     /locus_tag="VKU03_15405"
                     /EC_number="2.5.1.55"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019403638.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008676 - 3-deoxy-8-phosphooctulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-deoxy-8-phosphooctulonate synthase"
                     /protein_id="HLJ72703.1"
                     /translation="MDNHSIVEIGRGAAAVRFGARSPLAVIAGPCQLESRAHALEMAG
                     ALKAIAEKLGFGLVFKTSFDKANRTAGGAARGVGLEQALPIFAEIRESLGLPVLTDVH
                     EPSQCGLVAEAVDVLQIPAFLCRQTDLLVAAARTGRAVNVKKGQFLAPWDMKHVVAKI
                     AGAGGRDILVTERGASFGYNTLVSDMRALPIMAAETGAPVIFDATHSVQQPGGQGASS
                     GGQREFVAVLARAAVAVGVAGIFIETHQDPDRAPSDGPAMLPLGDFEALVGELMAFDR
                     LAKSRREPPQ"
     gene            5748..>6003
                     /locus_tag="VKU03_15410"
     CDS             5748..>6003
                     /locus_tag="VKU03_15410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72704.1"
                     /translation="MPAFIAGLVVLYLLLFGIKQFSRLSPAAAARLVRQGGGALGLLA
                     ALLLLLRGSVLLASLLASAALGFAGWRNAANLGAWFRGARR"
BASE COUNT         1079 a         1925 c         1979 g         1020 t
ORIGIN      
        1 ggcgacgcgc agcccctcgg cctgacccgc cgcgccttgc gcgatcgcgg caagccgatc
       61 gctccagccg ggcgagcgcc tgtcctgcgc cggcgccatg accgccagca tgagagcggc
      121 gtcggcgaca ttgttggtca ggggtccgat cgtgtcgaac atcgaagcga gcggaaccac
      181 gccggctgtc tcgacaaggc cgatcgtcgt cttcagcccg acgcagccgc agaacgaggc
      241 gggcaggcgc actgagccgc cggtgtcgct gccgagcgcc gccgggacca ggcccgccgc
      301 gaccgcgacg cccgagccgc tcgacgagcc gccgggcgtt cgcggatggc ctcgatcgcg
      361 cgggttgagc ggggcgccgg tcgtctcgtt cgagccccag ccgccgaagg cgtattcgac
      421 agtatgagtc ttgccgacaa tgatcgctcc ggcggcgcgg agacgcgcga cgaccggcgc
      481 gtccgcggcg gcgggggccg cgtccgccct tgtcggcgag ccggctttgg tcggcaggcc
      541 ggcgacgtcg ataatgtcct taacggcgat cggcatgccg gcgagcgggc cgggcgccac
      601 gccggccgca attagcgcgt cgacatgctc agcgtcgatc agcgcgcgtt tttcgtcgag
      661 cgcgacgaac gcgttgacga ctccattgtc gcgcttgatt gcgtcgagcg tgcgtttcgc
      721 gatgtcgacg gcgcttacgc cgccggccag ggcgtcgcgc gtctgtttga aggtaaggag
      781 ctcgtcgacc atcatcccgc catttcgaaa cgaccgtggc cgatcgcggg gcggcaggca
      841 aggggcagat cgacgctatc gtttcgcctg ctcgatcagg ccgcgcgcgc cggccagcag
      901 cttctcccat ccgaagagca gattggcgga gaagcgcaac tcgacattca gttcgccgag
      961 gcggaagtcg tcgatgcaga aattgggggt cgcgcgatgc tgatccggct tgccgcagcg
     1021 ggcggcgaat tcccggccct cgggcgcggc gaaataaagc tcctcccctt cgaacgggct
     1081 gccgggtagg aaaccgcgcg cgatcaagcc gcccggctgc gtccattcgt tcgagtcgag
     1141 aaagcgcgca tacagcttga ccgggcgatc ggcgggatcg agcgccgaat cggcctcgct
     1201 gatcgtcagg aaaacgagcc ggtcatgccg ctcggcgaga tcggtcgcgc tggcgatgtc
     1261 ctggaggtcg ccggccggcg cgaaatcggg atagaaggcg gcgaggctga gcttttcgag
     1321 atggccgccg ccgcgcgagc cgggtcgaaa atagccgcct ggcagcacga aagtcgatcc
     1381 gccgatcgtc gcgtggaccg ggccggagac ggccgccgga tggagcgccc gccataggcg
     1441 ataggcgccg gcgagcggca aaggcagcag gctgagcccg agcagaatgg cggcgacgcg
     1501 gagcggtggc ggcattggcg ccgatctcat ccagcgaatc aggatgtgct tggagagatt
     1561 agcgcgggcg gcccggcccg gtcgatgcga gtccttcaaa gtaaataatc gcttgattca
     1621 gttcaaattt acccaattga agaaaccata gattcagcct gacttttaaa ctcgtcttca
     1681 ttcacgaatg ttactgttca tgtcatcgta aggccgaaat aaataatcgg ctacgggcag
     1741 tcgaacaggc aggtgtgcaa tgaggaacgc ggttaaactt gaggaaacca caagacgatc
     1801 cgaccgaatt gctgagatca gcccggttta tcttgaggcg ctcggcatgg tcgagcggct
     1861 gcaccggcgg cttctcgacg ttatcaaaga cgaattcgac cggcgcggcc gctctgacgt
     1921 caacgccgtc caggctctgt tgctctataa tatcggcgac aaggaattga cggcggggga
     1981 actgcgcacg cgcggctatt atcttggctc gaacgtctcc tacaacgtca agaaaatggt
     2041 cgagatgggc tatctccatc acgcgcgttc gcgcatcgac cggcgctcgg tgcgcatcag
     2101 cttgaccgag aagggccagg aagtgcatca gatcgtcgcc gggctttacg agaagcacgc
     2161 ccgcacggtc gagcagatcg gcggcgtttc cgtcgacgat ttcctgcgca tgaaccagtc
     2221 gctcgcccgg ctcgagcgct tctggaccga ccagatcaaa taccgcctct gagcgcggcg
     2281 ctcgaaggat cagagggctt gagccgggct gcagccatgc ggcccggctg cttcgtccgg
     2341 aacgccgcta cttgcgctga ttgccggcga tcgcggcggt gacgccgagc ccgaccagca
     2401 gcgcgccgct cacatagcgc tcgaagcggc gataaaagac gccgcgcttc agccagccgc
     2461 cggcggcgct ggcgatgagc gcatagcagc cgtcggtgat gaagccgagg ccggcgaagg
     2521 cgaggccgag aaagacgatc tgcgtggcga catggccgcg gccgacctcg gcaaattgcg
     2581 gcaggaaggc gaagaagaac aacgccgtct tcggattgag cagattgacg atgaagccgt
     2641 cgcggaagag ccggcgatac gaatagcgct tcaggttcat cgccgcgtcg gcgccgtcgt
     2701 cgcggccgaa aatcttcttc aggccgagcc agacgagata agccgcgccg gcatatttga
     2761 ccgcgctgaa ggcgagcgcg gaggaggcga gcagcgccga gaggccgacg gcggccgcca
     2821 gcgcatggac caaggtcgcg gcgtggatgc cgagatcgga gacaagcccg gcgagccgtc
     2881 cctgctcggc cgagcgcgcg acgatgtaga gaaccgccgg tcctggaatc agcagcagga
     2941 ccaaagcggc cccggcgaac agcccaagat tggcggcgct cggaatgacg atcaccacgc
     3001 ttgggacgac gatctccatg acgaggctct cctgactttg gcgcgcagcg agcctcacgc
     3061 cgcccggcct gtcaatcccg gcttttcgcc gccgcaattt gtcgtgatcg cggcggcaaa
     3121 ggcttttccc gccccgcaag acataatatc gccaacctgc ttggcttttt taaccaggac
     3181 gcgataggaa atcgctggcg ttggccggac agggagggcg gtcgcgctga agcgacaagc
     3241 ggtccttcaa agaccgcgct ttcgcttaag atattttgac atggggcccg cctgccgatg
     3301 aaagacgtcc aattgccgcg cgtttgccgc cgcgcgtttc tttccgccgc tgcgactttc
     3361 gcgctgaccg cctttgccgg ctcggccttc gctcaacaga aattcgccgc ccaggccgaa
     3421 tggcgccaga actacgaatc gtccgcgcgt ctgacggtgc cgcgcgaatc gacgccggtc
     3481 ctgtcgccgg agactttggc cgcgaccgaa gcggcgatcc agcaatacca gggcattgtc
     3541 gagcatggcg gctggacgac ggtgccgacc ggacaagtgc tgaagcttgg agccaagtcc
     3601 aaggccgtcc aggcgctgcg ccagcgcctg gtcgtatcgg gcgatctcga ttccgttgcg
     3661 ggcgattcgc cggtcttcga ttcgttcgtc gaagcggcgg tcaagcgttt ccaggttcgg
     3721 cacggcatca gcccgaccgg aatcgtcaat gacgagacct tcaccgaatt gaacgtgccc
     3781 gccgagacgc ggcttcgtca attgcagatc aacgtcgttc gccttcgcgc cttttccggc
     3841 aatctcggcg agcggttcgt aatggtcaat attccggccg cggcggtcga gacggtcgag
     3901 aacaataccg tctattcgca ccatgtcgcc ggcgtcggca agatcgatcg ccagtcgccg
     3961 atcatgcagg ccaaggccgt cgagatcaat ttcaacccgt tctggaccgt gccggcttcg
     4021 ctgatcaaga aggacttgat cccgaaaatg caggccgatc ccaattatct gaccgaagag
     4081 aagatccgcg tcttcaacaa ggaaggccag gaagtctcgc cgagctcgat caattggaat
     4141 tcgctcgacg cgacgcagta caagtaccgg caggacacgg gcgccgacat caactcgctc
     4201 ggctcggtgc gcatcaacat ccccaacccg tacggcgtct atatgcacga cacgccgtcc
     4261 aagggcattt tcggcgacga tttccgcttc gtctcctcgg gctgcgttcg cgtgcaggac
     4321 gtgcgcgact acgtcgcctg gctgctgaag gacacgcccg gctggtcgcg cgaccagatc
     4381 gaccaggtga tcgattccgg ccagcggctc gacgtgaggc tggacaagcc ggtcaacgtt
     4441 tattgggtct atatcaccgc ctgggcgacg cccgacggcg tggtgcagtt ccggccggac
     4501 gtctatcagc gcgacggcgc cgggcccggc ccgctggcga cggcggtacc cgagaagccg
     4561 ctggcgacgg tgcaggaata agcaagccgc gcaaagacct tgttggtaag ttttgacaaa
     4621 cggcgcgaga agcatcgcgc cgttttcttg tctctcgggt gagttggcgg aaagcgcaaa
     4681 cgcctttaaa tctcgccgcc attcccgcga aagcgggaaa ccagaaaacc gctgtttctg
     4741 aggactggct tagtctgggc tcccgcgttc gcgggaatga cagtcgaagg ggtcgatgga
     4801 caatcattcg atcgtcgaaa tcggcagggg cgccgcggcc gtgcgcttcg gcgcgcgatc
     4861 gccgctcgcc gtcattgccg gcccgtgcca gctcgaaagc cgcgcccacg ctttggagat
     4921 ggcgggcgca ctgaaggcga tcgccgaaaa gcttggcttc ggcctcgtct tcaagacttc
     4981 gttcgacaag gccaaccgta cggctggcgg ggccgcgcgc ggcgtcggcc tcgaacaggc
     5041 gctgccgatc ttcgcggaaa tccgggaaag cctcggcctt cccgtcctca ccgacgtaca
     5101 cgagccgagc caatgcggcc tcgtcgccga ggcggtcgat gtcttgcaaa ttcctgcctt
     5161 tctctgccgg cagaccgacc ttctcgtcgc cgcggcgcgg acggggcggg cggtcaatgt
     5221 gaaaaaggga caattcctcg cgccttggga catgaagcat gtcgtcgcca agatcgccgg
     5281 cgccggcggg cgcgacattc tcgtgaccga gcgcggcgcg agcttcggct acaatacgtt
     5341 ggtctcagat atgcgcgcct tgccgatcat ggccgccgaa accggcgcgc cggtcatatt
     5401 cgacgcgacc cattcggtgc agcagccggg cgggcagggc gcctcttccg gcggccagcg
     5461 cgaattcgtc gcggtgctcg cccgcgcggc cgtagccgtc ggggtcgcgg gaattttcat
     5521 cgagacgcat caggatccgg atcgcgcgcc atccgacggg ccggccatgc tgccgctcgg
     5581 cgatttcgag gcgctggtcg gcgaattgat ggccttcgac cgcctcgcca aatcccggcg
     5641 cgagccgccg caatgacggc ccagcctatt gcggcaggtg cgcgagaccc cactttccca
     5701 tagggtatgt cgtcgccgct cttggcccgc cagggaagtt gtcgccaatg cccgctttca
     5761 tagccggcct ggtcgtgctc tatctgcttc tcttcgggat caagcaattc tcccgtttga
     5821 gccccgccgc ggcggcgcgc ctcgtccgcc agggcggcgg cgcgctcggc ctgctcgccg
     5881 ccttgctgct gttgctgcgc ggcagcgtgc tgctcgcgtc gctcctcgcc agcgcggcct
     5941 tgggctttgc cggctggcgc aacgccgcca atctcggcgc atggtttcgc ggcgccaggc
     6001 ggc
//
LOCUS       DATGJW010000509        12387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_826191, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000509 DATGJW010000000
VERSION     DATGJW010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12387
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_826191"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VKU03_15415"
     CDS             <1..477
                     /locus_tag="VKU03_15415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020180003.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein LutB
                     domain-containing protein"
                     /protein_id="HLJ72705.1"
                     /translation="CGACMNHCPVYHAVGGHAYGWVYPGPMGAVLTPTLIGVKEGGQL
                     PNASTFCGRCEEVCPVRIPLPGMMRHWREREFERHLSPASVRAGLAFWAFFAKRPALY
                     RLATSIGMRALHLLGRARGRFSFLPLAGGWTRYRDLPAPQGATFQSQWRAKSRPVQ"
     gene            474..1148
                     /locus_tag="VKU03_15420"
     CDS             474..1148
                     /locus_tag="VKU03_15420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009451549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lactate utilization protein"
                     /protein_id="HLJ72706.1"
                     /translation="MSARDDILANIRFSLGVAGAEAPRRLAVKERLAQAPAGIVPARG
                     QGDAAARAATFKAEAERAQASVAEVQSAADVPAEIARYLRDANLPATLRMGADERLAA
                     MPWSETALEVSRGASDGHDLNGVSAAFAGIAESGTLALCSGADNPTTINFLPDNHIVV
                     LFARDIAADYESVWRNLRVRYGKGAMPRTLNLITGPSRSADIEQTLLLGAHGPRRLHI
                     VMVKEG"
     gene            1223..2701
                     /locus_tag="VKU03_15425"
     CDS             1223..2701
                     /locus_tag="VKU03_15425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014892549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HLJ72707.1"
                     /translation="MIFRTMALSACAAALAFSAGRASADDLNNAPLPGELPPPVITAP
                     QAAQPATPSAAPAQSNPAPAPTPSAAGAEPEPVPIPTAPAMQGMPAPNQGAATAPKRK
                     RIVHPAPPRETALSSDPSPTLQPNTFFATAKASERYGAIADAGGWPVVPGPLKAGAKG
                     PAVAILRQRLAAEGDLEATAALGPNADQWDGAVTTAVKHFQSRMGLRETGIVEGATLK
                     EMNVPAATRFKQLASSAQRLAGVNFPFGDRYVVVNLPSLSVEAVENGRVAHHYVAIVG
                     GVEHQSPEISARISVINLNPTWTVPTSIIKKEIIPRMQRDPGYLAREKIRILDGAGNV
                     IDPRSVNWSTERAVNYTLRQDSGAGNSLGAIRIGMPNKLAVYMHDTPSKRLFGADYRF
                     LSHGCVRVQGVYDLAEWLLQGTPGGPTGVWDKPALLEKIKQGEREDIKLAKPVPVIWV
                     YLTGWANSDGLVHFRDDVYGVDSVGAAAASAAPKAASAAPAQ"
     gene            2800..3984
                     /locus_tag="VKU03_15430"
     CDS             2800..3984
                     /locus_tag="VKU03_15430"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017485853.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLJ72708.1"
                     /translation="MLHVNPAFSRIGEENAFAVLARAGELQRQGRDIINLGIGQPDFL
                     TPAHIVEAAVRALRDGQHGYTPATGLAPLREAVAADLKKRLNVDVSPDLVLIVPGGKV
                     TMYMAIVMFGVPGAEILYPDPGFPIYRSMIEYTGATPIPVPVREENGFAFSAEEVLSL
                     ITPRTRLVILNSPANPTGGVTPKAEIDKFVAGLARWPDVAVLSDEIYGQMTYDGLAHQ
                     TLLAYPEIRDRLILLDGWSKTYAMTGWRLGYSIWPKPLYDNARKLAVNSYSCVNAPAQ
                     YAGLAALTGPQDEVSAMVAEFDRRRAVVLEGLNKLPGVHAAAPKGAFYAFPNIAATGW
                     KAKPLASALLEEAGVATIGGPDFGVHGEGFIRLSYANSTENIQRALERIGDFLSRRNA
                     PR"
     gene            4012..4845
                     /locus_tag="VKU03_15435"
     CDS             4012..4845
                     /locus_tag="VKU03_15435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012494482.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LssY C-terminal domain-containing protein"
                     /protein_id="HLJ72709.1"
                     /translation="MQQERRRRSILRRAGIAALILAAIYLGLAYGLLPALWRHHERQK
                     GLDALPMTTVTALGIPGDALNVGLEGDEADVICAMEAAGWSPADPVTLKTSLKITGSV
                     LFDRPYAKAPVSPLYYLGRREDLAFEKAAGRSADERHHVRFWKVLDAGEDGSPVWLGA
                     ATFDRGVGVSHYTGQITHHIAPDIDAERDLISADLAAAKKVDTTYETSGVEPTVTLRN
                     GGGDFYYTDGEIRFSKLTQGCDSHNAQPASLPTSPLVQTRGWVWRHIVAPVAAFVHGA
                     G"
     gene            complement(4940..5803)
                     /gene="purU"
                     /locus_tag="VKU03_15440"
     CDS             complement(4940..5803)
                     /gene="purU"
                     /locus_tag="VKU03_15440"
                     /EC_number="3.5.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012045621.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008864 - formyltetrahydrofolate
                     deformylase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formyltetrahydrofolate deformylase"
                     /protein_id="HLJ72710.1"
                     /translation="MAGARYILTLSCANRPGIVAKVSTSLFESGANILDAQQFDDTET
                     GNFFMRVVFDAAAQPLELPALRGRFEAIAEPFAMTWSLRPSEEKKRVLILVSRFDHCL
                     VDLLYRWRSGELRMDVVGVGANYPRETYAHIDFGDIPFHYLPITKQTKLEQETQVWDL
                     VRTSKCDVVVLARYMQILSDGLVAKLQGRAINIHHSFLPGFKGAKPYHQAHERGVKLI
                     GATAHYVTSDLDEGPIIEQDVETITHRDTPEDLVRKGRDIERRVLSRALAHHLEDRVL
                     LNRNKTVVFAG"
     gene            6115..6789
                     /locus_tag="VKU03_15445"
     CDS             6115..6789
                     /locus_tag="VKU03_15445"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018261021.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042578 - phosphoric ester hydrolase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase family protein"
                     /protein_id="HLJ72711.1"
                     /translation="MRTYAVGDIHGCADLLIALLAAIRAAHPADRGCKLIFLGDLVDR
                     GPDSAKAAAMVHALQAQAPAIECLRGNHEQMMIDWLRAGEDLWLVNGGLDTIRSFGVE
                     AGGEEAFTEAVEWMESLPTWREDAAHVYVHAGLRPGRPYDRQSDQDRLWIREGFLDVE
                     HDFGKHVVHGHTPRLGGPERRPFRTNIDTGAVYGGALTAAVLDDDSPAPIGFLRVPDS
                     ASLRLA"
     gene            6839..7276
                     /locus_tag="VKU03_15450"
     CDS             6839..7276
                     /locus_tag="VKU03_15450"
                     /inference="COORDINATES: protein motif:HMM:NF021255.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antitoxin Xre/MbcA/ParS toxin-binding
                     domain-containing protein"
                     /protein_id="HLJ72712.1"
                     /translation="MGETSLYPQTRYAPSPLVDLTAKEERERLSPAAIRTFFNIMARW
                     GVRDEDARALLGGMSNGPFYDMKKNSERTLDADRLTRVSYLIGVFKALNILYSEALAD
                     RWVQLPNDNPIFAGRTPLAYMMKGGQPAMQTVRRLLDARRGGL"
     gene            7273..7953
                     /locus_tag="VKU03_15455"
     CDS             7273..7953
                     /locus_tag="VKU03_15455"
                     /inference="COORDINATES: protein motif:HMM:NF020387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RES family NAD+ phosphorylase"
                     /protein_id="HLJ72713.1"
                     /translation="MTLPPTSLVRRYDTHRLIPAKYSAGGDSVLVRVAASDADLPQIF
                     DLDNATNDRLLAENGYLPGIGVYELVFGVPYYRIVNAAFCHAHPLGSRFNGPDRGAWY
                     AAFELETSQAEIAYHKQCELLEVKWREEEALYDDYLADFSAELHDLRVSSEFQACLDP
                     DSYVASQELAQRLLEAGALGVVYPSVRKAGGTCLACFRPALVANVRKSGSYLFRWDGK
                     RATVEPRV"
     gene            complement(7980..8768)
                     /locus_tag="VKU03_15460"
     CDS             complement(7980..8768)
                     /locus_tag="VKU03_15460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72714.1"
                     /translation="MPAALGPEVLRAEHVEKSFGTIAALKDVNLQLRRGEVLGLLGDN
                     GAGKSTLMKIFTGCHQPSAGQLYFEGKPIQFRSVSHARSLGIEAVYQDLALVNQLNVY
                     RNMFLQREPVRGGFLGVLDERQMRRRAIELLERMQVRIPSVDVEVAKLSGGQRQAVAI
                     ARSVYQKAKLLLLDEPTAAMGAKESALILDLIQRLKEEGDIAMIIIAHNYAQIFDVCD
                     RINLVEGGVIAFDKRTSETSVQELTDRVVREYRSAREFVQQPRR"
     gene            complement(8768..9727)
                     /locus_tag="VKU03_15465"
     CDS             complement(8768..9727)
                     /locus_tag="VKU03_15465"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72715.1"
                     /translation="MLRRLGGIVLRWREASILLAGIILAAYFQSTNSAFLSLSNIENL
                     IDYTATTSIIAAGEVMVLVCGELDLSAGMVYALSPFIMYFAAAAGLPAGAAIVLALAA
                     AGLVGLINGAVTTLLSVPAFVTTLGTMFLINGLTLTISGGFPVQPNAAAAFAAAFGGN
                     AWARLFWALGIAVLLQFLLSRTRFGLHTIATGGNIVAAAEVGIRVRSIKIRNFVLCSA
                     LGGFAGVIDAFRIGSIDPLAGGSEITFAAIASAVIGGTLLTGGSGTLIGAFIGALVLG
                     ILKDGFTLSGVSAFTFDMILGAAILATMALNVRLTRWRDRGET"
     gene            complement(9812..10909)
                     /locus_tag="VKU03_15470"
     CDS             complement(9812..10909)
                     /locus_tag="VKU03_15470"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ72716.1"
                     /translation="MTKTFDINAPARLSPSVSRRSLFRGGLSSGAAFFALSALGGSEA
                     AWAAGASGGLPAHPEWKFVFVNHVTTNPFFVPTQYGIADACSLLGCTYQWTGSQTSVA
                     SEMVNAMNAAITAKADAIAVSLVDLHAFNEPVKRALEAGIPVFSYNADAKGNERLAYI
                     GQDLYASGQALGRRIVDLVGEGHVVGFIATPGQLNIQPRLDGAKDAIKASGKKIVLDE
                     VATGPTVNEEVSRVDAYYVGHKDVKGMFAVDAGSTMAVGKAMEKYGLHAKGVHAGGFD
                     LLPGTLDAIANDHLDFTIDQQPYLQGFYTLMEMAMFKLSGGLTGPADINTGLKFVTKS
                     SVEPYRTTHSRYEGNSSAEAVIPRSGPISGA"
     gene            complement(11461..>12387)
                     /locus_tag="VKU03_15475"
     CDS             complement(11461..>12387)
                     /locus_tag="VKU03_15475"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007198496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HLJ72717.1"
                     /translation="QIIVGTGGKHILFNALLATVNPGDEVIVPAPYWVSYPDMVLIAG
                     GTPVFVDTKIENGFKLKAEDLEKTITPKTKWLILNSPSNPSGAAYTEAELKAIADVLM
                     RHKHVWALTDDIYEHLVYGDFAFKTIAEVEPNLIERTLTMNGVSKSYAMTGWRIGYAG
                     GPDKLIKAMDMLQGQQTSGACSIAQWAAVEALNGPQDFIPKARKVFEERRDLVVSMLN
                     QARHLKCPTPEGAFYVYPSVAGAIGKKSPTGKVIGNDEDFVNELLETEGVAVVHGSAF
                     GLGPNFRVSYATSTEVLEDACKKIQRFTAELR"
BASE COUNT         2133 a         4065 c         4021 g         2168 t
ORIGIN      
        1 tgcggcgcct gcatgaacca ttgcccggtg taccatgcgg tcggcggcca tgcttacggc
       61 tgggtctatc ctgggccgat gggcgcggtg ctgaccccga ctctgatcgg cgtgaaagaa
      121 ggcggccaat tgcccaacgc ctcgaccttt tgcggccgct gcgaggaagt ctgcccggtc
      181 cgaatccccc tgccgggcat gatgcgccat tggcgggagc gcgaattcga gcgccatctg
      241 tcgccggcct cagttcgcgc cggcctcgcc ttctgggctt ttttcgccaa gcgtccggcg
      301 ctctaccggc tggcgacgag catcggcatg cgcgcgctgc atcttctcgg ccgcgccagg
      361 ggccgctttt cgttcctgcc gctcgccggc ggctggacgc gctaccgcga cctgccggcg
      421 ccgcagggcg cgacattcca gagccaatgg cgcgccaaga gccggccggt gcaatgagcg
      481 cgcgcgacga cattctcgcc aatatccgct tttcgctcgg cgtcgccggc gccgaggcgc
      541 ctcgacggct cgccgtgaag gaaaggctgg ctcaggcgcc ggcggggatt gtcccggcgc
      601 gcggccaggg cgacgccgcc gcgcgggccg cgaccttcaa agccgaagcc gaacgggcgc
      661 aggcgagcgt tgcggaagtg caaagcgccg ccgacgttcc ggcggaaatc gcgcgttatc
      721 tgcgcgacgc caacctgccg gcgacgctgc ggatgggcgc ggacgagcgt ctcgccgcca
      781 tgccgtggag cgagacggcg ctcgaagttt cgcgcggcgc ctccgacggc cacgatctga
      841 acggagtcag cgccgccttt gccgggatcg ccgaatccgg gacgctcgct ctgtgctcgg
      901 gcgccgacaa tccgaccacg atcaacttcc tgcccgacaa tcacatcgtc gtgcttttcg
      961 cccgcgacat cgccgccgac tacgaaagcg tgtggcggaa tttgcgcgtc cgctacggca
     1021 aaggcgccat gccgcgcacg ctcaacctga tcaccggccc gtcgcgctcg gccgacatcg
     1081 agcagacgct gctcctcggc gcgcatgggc cgcggcgcct gcatattgtc atggtgaaag
     1141 agggctgagc cggctgcgtt tacccctgaa acatgtttca aggccatgat cgcgcccgga
     1201 ttctaggcga atcggcagcg gaatgatttt tcgaaccatg gctttgagcg cctgcgcggc
     1261 ggccctcgcc ttctcggcgg gccgcgcctc ggcggacgat ttgaacaacg cgcccctgcc
     1321 gggcgagttg ccgccgccgg tcatcacagc gccgcaagcg gcccagccgg ctacgccgtc
     1381 cgcggcgcct gcccaatcaa atcctgcgcc cgcgccgacg cccagcgccg ccggcgccga
     1441 gcccgagccg gtgccgatcc cgaccgcgcc ggcgatgcag ggcatgccgg cgccgaatca
     1501 aggcgccgcg acggcgccca aacggaagag aatcgtccat cccgcgccgc cgcgcgagac
     1561 ggctttgtcg agcgatccca gtccgacgct gcagcccaat acgttcttcg ccaccgccaa
     1621 ggcctccgag cgctatggcg cgatcgccga tgccggcggc tggccggtcg tgccggggcc
     1681 gctgaaagcc ggcgcgaaag ggccggccgt ggcgatcctg cgccagcgcc tcgccgccga
     1741 gggcgatctc gaggcgacgg cggcgctcgg gccgaacgcc gatcaatggg atggggcggt
     1801 caccaccgcg gtcaagcatt tccaatcgcg catgggcttg cgcgagacgg gaatcgtcga
     1861 aggggcgact ctgaaagaga tgaacgtacc cgccgcaacg cggttcaagc agctcgcctc
     1921 gagcgcgcag cgcctcgccg gcgtgaactt tccgttcggc gaccgctatg tcgtcgtcaa
     1981 cctgccgtcg ctctcggtcg aggccgtcga gaacggcagg gtggcgcatc attacgtcgc
     2041 gatcgtcggc ggcgtcgagc atcaatcgcc ggaaatttcg gccaggatct cggtgatcaa
     2101 tctcaacccg acctggaccg tgccgacctc gatcatcaag aaagaaatca ttccgcggat
     2161 gcagcgcgat cccggctatc tcgctcgcga gaaaatccgc atcctcgacg gcgcgggcaa
     2221 tgtcatcgat ccgagaagcg tgaattggag caccgagcgc gccgtgaact atacgttgcg
     2281 gcaggattcg ggcgcgggca attcgctcgg cgcgatccgc atcggcatgc ccaacaagct
     2341 cgccgtctac atgcacgata cgccgtcgaa gcgccttttc ggcgcggact atcgcttcct
     2401 gtcgcatggc tgcgtgcgtg tccagggcgt ctacgacctc gccgaatggc tgctgcaagg
     2461 aacgccgggc ggcccgaccg gcgtttggga caaaccggcg ctgctcgaaa agatcaagca
     2521 aggcgagcgc gaggacatca agctcgccaa gcccgtgccg gtgatctggg tatacctgac
     2581 cggctgggcc aatagcgacg ggctggtcca tttccgcgac gatgtctatg gcgtcgattc
     2641 ggtcggcgcc gccgccgcca gcgccgcgcc gaaagccgcc agcgccgccc ccgcgcagtg
     2701 agtacctccc ttctcctcgc aaagcggggg gaagggaaag ccgcccttga tccgcgccgc
     2761 ccttcgcggc agataggcgc gtcaggaaga ggaagcgcca tgctgcatgt caatcccgcc
     2821 ttctcgcgca tcggcgagga aaacgccttc gctgttctcg cccgggccgg cgaattgcag
     2881 cgccagggcc gcgacatcat caatctcggc atcggtcagc cggatttttt gacgccggcc
     2941 catattgtcg aggcggcggt cagggccctg cgcgacggcc agcatggcta tacgccggcg
     3001 accggcctcg ctcctctgcg cgaagcggtt gcggcggacc ttaaaaagcg cctcaacgtc
     3061 gacgtctcgc ccgatctcgt cctgatcgtc ccgggcggca aggtgacgat gtatatggcg
     3121 atcgtgatgt tcggcgtgcc gggcgcggag atcctctatc ccgatcccgg cttcccgatc
     3181 tatcgctcga tgatcgaata taccggcgcg acgccgatcc cggtcccggt gcgcgaggag
     3241 aacggcttcg ctttctccgc cgaggaggtc ttgagcctga tcacgccgag gacccggctg
     3301 gtcattctca attcgccggc caatccgacc ggcggcgtca cgcccaaggc ggagatcgac
     3361 aaattcgtcg ccggcctcgc ccgctggccg gacgtggcgg tgctctccga cgaaatctac
     3421 ggccagatga cctatgacgg cctcgcccat cagacgctgc tcgcctatcc ggagattcgc
     3481 gatcgcctga ttctgctcga cggctggtcg aaaacgtacg cgatgaccgg ctggcggctc
     3541 ggctattcga tctggccgaa gccgctctac gacaatgcgc gcaagctcgc ggtcaattcc
     3601 tattcctgcg tcaacgcgcc ggcgcaatat gccggccttg ccgctctgac cggaccgcag
     3661 gacgaggtca gcgccatggt cgccgaattc gatcgccgcc gcgccgtcgt cctcgaaggg
     3721 ctgaacaaat tgccgggcgt ccatgcggcg gcgccgaaag gcgccttcta cgcgttcccc
     3781 aacatcgcgg cgaccggctg gaaggcgaag cccctcgcct cggctttgct cgaagaggcc
     3841 ggcgtcgcga cgatcggcgg accggatttc ggcgttcatg gcgaaggctt cattcgcctc
     3901 tcctacgcca attcgaccga gaacattcag cgcgcgctcg agcggattgg cgatttcctg
     3961 agccggcgca acgcgccgcg ctgacgcggc cttcgcttga ccgcgccacg cgtgcagcaa
     4021 gagaggcgcc gccgttcaat tctccggcgc gccggaatcg ccgcgctgat cctcgccgcg
     4081 atctatctcg gcctcgctta cggcctcctt ccggccctct ggcgtcatca cgagcgccag
     4141 aaaggactcg acgctttgcc gatgaccacc gtcaccgcgc tcggcattcc cggcgatgcg
     4201 ctcaatgtcg ggctcgaagg cgacgaagcg gacgtgatct gcgccatgga ggcggccggc
     4261 tggtcgccgg ccgatcccgt gacgctgaaa accagcctca aaatcaccgg cagcgtcctg
     4321 ttcgaccgcc cctacgccaa ggcgccggtc agccccctgt actatctggg ccgccgcgag
     4381 gacctcgcct ttgaaaaggc ggcggggcgc agcgccgacg agcgccacca tgtccgcttc
     4441 tggaaggttc tcgatgccgg cgaggacggc tcgccggtct ggctcggcgc ggcgacgttc
     4501 gatcgcggcg tcggcgtcag ccactatacc ggccagatca cccatcatat cgcgcccgat
     4561 atcgatgcgg agcgcgatct catttccgcc gatctcgccg cggcgaagaa agtcgacacg
     4621 acttacgaga cgagcggcgt cgagccgacg gtgacgctcc gcaatggcgg cggcgatttc
     4681 tactataccg acggcgagat ccgcttcagc aagctgacgc agggctgcga tagccataat
     4741 gcgcagcctg cgagcctgcc aacgtcgccg ctcgttcaaa cgcgaggctg ggtctggcgc
     4801 catatcgtcg cgccggtcgc ggcgtttgtg catggcgctg gttaagccgc cattgcgcgc
     4861 gaaatgaagc aatccatcca taggggcggc gccctgtgaa tggattgctg cgttgcttcg
     4921 ctcctcgcaa tgacgagttt tagcccgcaa acacaaccgt cttgttgcgg ttgagcagca
     4981 cgcgatcctc gagatgatgc gccagcgcgc gtgagagcac ccgccgctcg atgtctcgcc
     5041 ccttgcgcac gaggtcttcc ggcgtgtcgc gatgggtgat cgtctccacg tcctgctcga
     5101 tgatcggccc ctcgtcgaga tcggatgtga cgtaatgcgc cgtcgcgccg atcagcttga
     5161 cgccgcgctc atgcgcctga tggtacggct tggcgccttt gaagccgggc agaaacgaat
     5221 ggtggatatt gatcgcccgc ccttgcagct tggcgaccaa tccgtcggac aggatctgca
     5281 tatagcgggc gagcacgacg acgtcgcatt tcgacgtacg gaccaaatcc cagacttgcg
     5341 tctcctgttc cagcttcgtc tgcttggtga tcggcaggta gtggaacgga atgtcgccga
     5401 aatcgatatg ggcgtaggtc tcgcgtgggt agttggcgcc gacgccgacc acgtccatcc
     5461 tgagctcgcc gctgcgccag cgatagagaa gatcgacgag gcagtgatcg aagcgcgaga
     5521 cgaggatgag gacgcgcttt ttctcctcgc tcggccgcag ggaccaggtc atcgcgaaag
     5581 gttcggcgat cgcctcgaaa cgcccgcgta gggcgggcag ctcaagcggc tgggcggcgg
     5641 cgtcgaagac gacgcgcatg aagaaattgc cagtctcggt gtcgtcgaat tgctgcgcgt
     5701 cgagaatgtt cgcgccgctc tcgaagaggc tcgtcgagac tttcgcgacg atgccgggcc
     5761 ggttggcgca ggaaagggtg agaatgtatc gagcccccgc catacgattt cgcctttcct
     5821 gacgcgccgc cccggggcgg cttcgaccgc cttcaatggc cgcaacaacg ccttgatcgt
     5881 caagagaaaa ccttttttcc ggaggctcgc gcgccaagaa aattgagcta ggcttatgac
     5941 cggccgcctc gccgctccgg attggacctt gagccgcctc gtgatagcgc tcggagagct
     6001 tttggacgtg aagcgttaat ggcgtcgcgg ctggagcaat tcctcgacag gacggccggc
     6061 gaggccgtcg aggctttcgc ccgcgcctgc cgtggcgcgc ctcagtcggg cgcgatgcgg
     6121 acttacgcgg tcggcgacat tcatggctgc gccgatcttc tcatcgccct gctcgccgcc
     6181 attcgcgccg cccaccccgc cgatcggggc tgcaaactca tctttctcgg cgatctcgtt
     6241 gaccgcggtc ccgacagcgc caaggcggcg gcgatggtcc atgcgcttca ggcgcaggcg
     6301 cctgctatcg agtgcctgcg cggcaatcac gagcagatga tgatcgactg gctccgggcg
     6361 ggcgaggatc tgtggctcgt caatggcggc ttggacacga tccggtcgtt cggcgttgag
     6421 gccggaggcg aagaggcctt tacggaagct gtcgaatgga tggagagcct gccgacctgg
     6481 cgggaggacg cggcgcatgt ctatgtccat gccggcctgc gcccgggccg gccctatgac
     6541 cgccagagcg accaggatcg cctatggatt cgcgaaggct ttctcgatgt cgagcatgat
     6601 ttcggcaagc acgtcgtcca cggccatacg ccgcgtcttg gcggccccga gcgacgcccg
     6661 tttcgcacca atatcgatac tggcgcggtc tatggcggcg ccttgaccgc cgcggttctg
     6721 gacgacgatt cgcccgcgcc gatcggattt ttacgggttc ctgattcggc gagtttgaga
     6781 ttggcttgat ttttctccta ttcaggagta atttgttaca aatttaggag aaatcgcgat
     6841 gggcgaaacc tccctttatc cgcagacgcg ctacgcgccg tcgccgctcg tcgacttgac
     6901 cgccaaggag gagcgcgagc ggctgagccc ggccgcgatc cgaaccttct tcaacatcat
     6961 ggcgcggtgg ggcgtccgcg acgaggacgc ccgcgccttg ctcggcggca tgtcgaacgg
     7021 cccgttctac gacatgaaga agaattccga gcggacgctg gatgcggacc gattgactcg
     7081 cgtctcctac ctgatcggcg tcttcaaggc gctcaacatc ctctattcgg aggcgctggc
     7141 cgatcgctgg gtgcagcttc cgaacgacaa tccgattttc gccggccgaa cgcctctggc
     7201 ctatatgatg aaaggcgggc agccggcgat gcagacggtg cggcgcctgc tcgacgcgcg
     7261 ccgcggcggc ctgtgaccct gccgccgaca agccttgtcc ggcgctacga tacccatcgc
     7321 ctgatcccgg ccaaatacag cgcgggcggc gacagcgttc tcgttcgcgt cgcggcgagc
     7381 gacgccgacc tgccgcaaat tttcgacctc gacaacgcga ccaacgatcg cctgctcgcc
     7441 gaaaacggct acctgcccgg catcggggtc tacgagctcg tcttcggcgt cccctattat
     7501 cgcatcgtga acgccgcctt ctgccatgcg catccgctcg gcagccgttt caacgggccg
     7561 gatcgcggcg cctggtacgc ggcgttcgaa ttggagacgt cgcaggccga gatcgcctac
     7621 cacaagcaat gcgagctgct cgaagtgaag tggcgcgaag aggaggcgct ctatgacgat
     7681 taccttgccg atttcagcgc cgaattgcac gatctgcgcg tttcttccga attccaggcg
     7741 tgtcttgatc ccgacagcta tgtcgcctcg caagagctcg cccaaaggct cctcgaagcg
     7801 ggcgctctcg gcgttgtcta tccgagcgtc cgcaaggcgg gaggaacctg cctcgcctgc
     7861 ttccggccgg ccttggtcgc caatgtccga aaaagcggct cgtacctctt ccgatgggat
     7921 gggaagcggg cgaccgtcga gccgcgcgtt tgaggccggc ggccgcttcg cgccgattct
     7981 caccggcgcg gctgctggac gaactcgcgc gccgaccggt attcgcgcac gaccctgtcg
     8041 gtcagctcct gaaccgaggt ttcgctggtg cgcttgtcga aggcgatcac gccgccctcg
     8101 acaagattga tgcgatcgca cacgtcgaaa atctgcgcgt aattatgggc gataatgatc
     8161 atcgcgatat cgccctcctc cttcagccgc tggatcaggt cgaggatcag cgccgattcc
     8221 tttgcgccca tggcggcggt cggctcgtcg agcagaagca atttcgcctt ttgatagacc
     8281 gaccgcgcga tggcgacggc ttggcgctgt ccgcccgaca gcttggcgac ctcgacgtcg
     8341 acggacggaa tcctcacctg catgcgctcg agcagctcga tcgcccgccg gcgcatctgg
     8401 cgctcgtcga gcacgccgag gaatccgccg cggaccggct cgcgctggag gaacatgttg
     8461 cgatagacat tgagctggtt gaccagggcc aggtcctgat agaccgcctc gatgccgagc
     8521 gagcgggcgt ggctgaccga gcggaattga atgggcttgc cctcgaaata gagctgcccg
     8581 gcgctcggct gatggcagcc ggtgaaaatc ttcatcaggg tcgactttcc ggcgccgttg
     8641 tcgccgagca agccgaggac ctcgccgcgg cggagctgca gattgacgtc tttcagcgcc
     8701 gcgatcgttc cgaaggattt ttcgacgtgc tcggcgcgaa gcacttccgg tccgagggca
     8761 gccggcatca cgtctctccc cggtcgcgcc accgcgtcag gcggacattg agggccattg
     8821 tcgcaaggat ggcggcgccc aaaatcatgt cgaaggtgaa ggcgctcacc ccggaaagag
     8881 taaagccgtc cttgagaatc ccgagcacca gggcgccgat aaaggcgccg atcagcgttc
     8941 ccgagccgcc ggtcagcaag gtgccgccga tcaccgccga agcgatcgcg gcgaaggtga
     9001 tctcggagcc gccggcgagc ggatcgatcg atccgatacg aaaggcgtcg atgacgccgg
     9061 caaaaccgcc gagcgcgctg cacaggacga aattgcggat cttgatgctg cgcaccctga
     9121 tcccgacttc ggccgccgcg acgatgttgc cgccggtcgc gatcgtgtgc aggccgaagc
     9181 gggtgcggct cagcaggaac tgcagcagga cggcgatgcc gagcgcccag aacagccgcg
     9241 cccaggcgtt gccgccgaac gcggcggcga aggcggcggc ggcgttcggc tggaccggaa
     9301 agccgccgga gatcgtcaag gtcaaaccgt tgatcaggaa catcgtgccg agcgtcgtca
     9361 cgaaagccgg cacgctgaga agcgtcgtga ccgcgccgtt gatcaggccg accaggcccg
     9421 ccgccgccaa ggcgaggacg atcgccgcgc ccgccggcag accggcggcg gccgcgaaat
     9481 acatgatgaa gggcgacagc gcatagacca tccccgccga cagatcgagc tcgccgcaga
     9541 ccaggaccat gacttcgcca gcggcgatga tcgaggtcgt cgcggtgtag tcgatcagat
     9601 tctcgatgtt ggagagcgac aggaaggcgg aattggtcga ctgaaaatag gccgcgagga
     9661 tgatcccggc gagaaggatg ctcgcctcgc gccagcgtaa gacgattccg cccaggcggc
     9721 ggagcaaagg cggccgcccc tcgcgagcgc gatgcggacc ggacgcgccg gcaaggggcc
     9781 tcgcctcgtc ccgcatcgcc gcgctctttc gtcaggcgcc gctgatcggc ccagaccgtg
     9841 gaatgacggc ttccgccgac gagttgccct cgtagcggct atgggtcgtc cgatacggct
     9901 cgacgctgct cttggtgacg aacttcaatc cggtgttgat gtcggccgga ccggtgaggc
     9961 cgccggaaag cttgaacatc gccatctcca tcagcgtgta gaagccctgc agataaggct
    10021 gctggtcgat ggtgaaatcg agatggtcgt tggcgatcgc gtccagcgtg cctgggagga
    10081 gatcgaagcc gccggcgtga acgcccttgg cgtgaagccc gtatttctcc atcgccttgc
    10141 cgaccgccat cgtgctgccg gcgtcgaccg cgaacatgcc tttcacgtcc ttgtgcccga
    10201 cataataggc gtcgacccgc gagacttcct cattgacggt cggaccggtg gcgacctcgt
    10261 cgagaacgat cttcttgcct gacgctttga tcgcgtcctt ggcgccgtcg agacgcggct
    10321 ggatgttgag ctggccgggc gttgcaatga agccgacgac atggccttcg ccgaccagat
    10381 cgacgatccg ccggccgagc gcctgtccgg aggcgtagag gtcctgtccg atataggcga
    10441 ggcgctcgtt gcccttggcg tcggcattgt acgaaaagac cgggatgccc gcctccaggg
    10501 cccgcttgac cggttcgttg aacgcatgca ggtcgacgag cgagacggcg atcgcgtccg
    10561 ccttcgccgt gatcgccgcg ttcatggcgt tgaccatctc ggaggccacc gaggtctgcg
    10621 atccggtcca ttgatacgtg cagcctagca gcgagcatgc gtcggcgatg ccatattgcg
    10681 tcggcacgaa gaacggattc gtcgtcacat gattgacgaa gacgaatttc cactctggat
    10741 gggccggcag gccgcccgac gcgccggccg cccaggcggc ctcgcttccg ccaagcgccg
    10801 aaagcgcgaa aaaagcggcc cccgaactga ggccgccgcg gaaaaggctg cggcgcgaga
    10861 cgcttggcga aagccgagcc ggggcgttga tatcgaatgt cttggtcatg ttgggcactc
    10921 cctctcattg ggctcaaaac gcgacgcgca gggctgcggg tccctcacga gcgcgacgct
    10981 ttttgcgggg aagacagatg gggatcgcgg ccggggcgca ggtttcgccc gaccgaggag
    11041 ggccaacgtc gtcaggtgaa tccggaagag gccgcgcccc ggcggtggag acgccaagcg
    11101 cttcgcgtgg gcgtcgccgc ctggcgcggc gccaggcgat tcagggcgca agcgccgacg
    11161 cgcggtgctg cgctgctcgc aaagagagtc tgacggatcg cgccggcgaa gctcttcaca
    11221 aaagcgtcct cccggttgtg gcgtttcgga agatcgaagc gcgccaggaa gcaattgtca
    11281 gattatatga acggacaaga tcgccgattt gtggcgccga gattatccgg cgctgtccgg
    11341 caaattaaaa ctccgtaatc ctgccggaga accccggctc gcgcctgggc gcgcgtttct
    11401 tcgcccccat tcattgccgc tttcgcggca atgaatgggg cgggcgcttt cgcgcgccgc
    11461 ttagcgcaat tcggcggtga agcgctggat tttcttgcaa gcgtcttcga gcacctcggt
    11521 cgaggtggcg taggagacgc ggaagttcgg gccgaggccg aaggccgagc catgcaccac
    11581 ggcgacgcct tccgtctcga gcaattcgtt gacgaaatcc tcgtcgttgc cgatcacctt
    11641 gccggtcggc gacttcttgc cgatcgcgcc ggcgacggac ggatagacat agaaagcgcc
    11701 ttccggcgtc gggcatttca aatgccgcgc ctggttgagc atcgagacga cgagatcgcg
    11761 ccgttcctcg aagacctttc ttgccttcgg aatgaaatcc tgcggcccgt tcagcgcctc
    11821 gacggcggcc cattgcgcga tcgagcaggc gccggacgtc tgctggccct gcagcatgtc
    11881 catggctttg atcagcttgt ccgggccgcc ggcatagccg atgcgccagc cggtcatcgc
    11941 gtagcttttc gagacgccat tcatggtcag cgtgcgctcg atcaggttcg gctcgacctc
    12001 agcgatcgtc ttgaaggcga agtcgccata gacgagatgc tcgtaaatgt cgtcggtcag
    12061 cgcccagaca tgtttgtggc gcatcaagac gtcggcgatc gccttgagct cggcttccgt
    12121 gtaggccgcg ccggacgggt tcgacggcga gttgaggatc agccatttcg tcttcggcgt
    12181 gattgtcttc tcgaggtcct cggccttcag cttgaagccg ttctcgatct tggtgtcgac
    12241 gaagaccggc gttccgccgg cgatcagaac catatccggg tacgacaccc aataaggcgc
    12301 cggaacgatc acctcgtcgc cgggattgac cgtggcgagc agcgcattga acaggatgtg
    12361 cttgccgccg gtgccgacga tgatctg
//
LOCUS       DATGJW010000510         6192 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_614275, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000510 DATGJW010000000
VERSION     DATGJW010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6192)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6192)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6192
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_614275"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..403)
                     /locus_tag="VKU03_15480"
     CDS             complement(<1..403)
                     /locus_tag="VKU03_15480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018064047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mandelate racemase/muconate lactonizing enzyme
                     family protein"
                     /protein_id="HLJ72718.1"
                     /translation="MAKIDRVEVLMVDLPPKVKRTDAIQAFVSQETPIVRIFGDDGVV
                     GTGYSYTIGTGGHAVVDLIARHLAPCLIGRDPAMIEAIWRDLLFHTHATSVGAITSLA
                     LCAIDMALWDMRCLRNGRPLHIEAGGAQSAVP"
     gene            complement(396..1934)
                     /locus_tag="VKU03_15485"
     CDS             complement(396..1934)
                     /locus_tag="VKU03_15485"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ72719.1"
                     /translation="MRLAIDGIGKNFPGVVALDGVSFDVRPGEIHALMGENGAGKSTL
                     IKIITGLYRPDRGRLLIDDRPAAFASPRDAIAAGISAVHQERNLIPRFSVGENIMIEN
                     LPIRRGLVDYDRAHAEARRFLDLIDPSIDTKAEVRRLSVAQMQIVEIAKALSLEASLL
                     LLDEPTASITDHEAQALFAVLRRLKNEGKGIVFVSHKLEEVIAISDRVTVLRDGKIAA
                     AGEPIAAMSRSRIVSAMIGREERVADIGARKPSDARALEARSVATSLGHKDITFTLRR
                     GEILGLYGLVGSGRTELARALIGDAHILAGEIRLNGAPVSISSISNAVRRHRIGYISE
                     DRKSEGLILAHSVRANVAITIWRQIAARFGLLTRGREAGAAAPLARKLDIRTPSLEQQ
                     VGKLSGGNQQKVSIAKWLAAGVDILIIDEPTVGIDVRTKTAIHELIAEITRSGVAVLL
                     ISSDMPEMITLADRILVMRDFALVGEIENERRYESMSRRIMSFIHEGVDSSGGDAQAE
                     AGHG"
     gene            complement(1939..2892)
                     /locus_tag="VKU03_15490"
     CDS             complement(1939..2892)
                     /locus_tag="VKU03_15490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72720.1"
                     /translation="MTLSRRFFSQEWSTLAVAILIGFAALSLLSPAFLTEYNLYVMLR
                     SFCVVLVVAFAQMLTLAVGQLNLAIGALGGLVAIAVGGMMEKLGFPIGAAVALGLLIG
                     IGGGLLNGLLTVRTGINGFIITLATASVFAGINLGVTESIPFYNLPRSFVAFGVERFF
                     AAPYLLIPPVIAALLLALFLARTAPGRYLLAVGGNPHAAELSGIPRDRIIVLAHALSG
                     LLAAIGAILAEAQLGSAQPTIGADWLVMSFAAPIIGGAALTGGHISVAATALAVVLIV
                     LIQNGMVLARVDPYWVQFLLGALILAAVGLNRLRAARIEVG"
     gene            complement(2889..3923)
                     /locus_tag="VKU03_15495"
     CDS             complement(2889..3923)
                     /locus_tag="VKU03_15495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184886.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72721.1"
                     /translation="MAPSQQRKTGMSLSIDTEFRSGRNAWLTEQSGAIGLAAIVLLFW
                     IVFASLAPGFVSSFNLFAMSRTLAIDIVIGFSTMVVLATGGMNLAIGSIGVCSVMLAG
                     YLMQKWGLPSPLALAAGLLLGALLGWANGFAIVRSGVNSFIITLASASLFTGGMLILT
                     KAVIFNGLPPAIAAFGRMRFAIVSPLLIIALAIGAALIVLFRFTALGREILAAGANPR
                     AAELSGVPVGRVIVIAHTLSGLLAAAAGLMLLTRLGAAMPSIGQDWLLPSFLAPVLGG
                     ALLSGGSISVIGAIIGALLVTTIRSGLLIMQVGNFWLQLFLGVMLLIAVLLDRYRTVF
                     AERRSLGRRP"
     gene            complement(4028..5071)
                     /locus_tag="VKU03_15500"
     CDS             complement(4028..5071)
                     /locus_tag="VKU03_15500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018184887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ72722.1"
                     /translation="MKRSAGRALFRALPILAAILCLPVGAAEASNNKIALIPGGPHPY
                     FAPWEQAAADAKKDFNIAAVDFKVPTEWKLNLQTELIESLLTQGYNGFGIFPGDAVGI
                     NTTIEELAANNAPALALAGCAQDPTKAAFCLGTDVYNSAYLGTKALIKAMGGKGKIVH
                     LAGLLVDPNTKLRMDAVEKAVKETGGAVTLIQHITDTDSQEAADQKINALLGAQKDQI
                     DGLIATAYIPSVVAAKSLRNLGDKRIKLVGIDDDKIVLDAIKDGFAAGTMAQNPYGQG
                     YIGAYVLDLLASGVCKVKADAPFIKTPQTARFIDSGTLLISADNIGSYKDELKKITND
                     IRGSMKTKYLDCK"
     gene            complement(5295..>6192)
                     /locus_tag="VKU03_15505"
     CDS             complement(5295..>6192)
                     /locus_tag="VKU03_15505"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008872493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HLJ72723.1"
                     /translation="VRDHWHTMTRTGKSARLSQHLAEPYIEIHPADAARLGIAGADIV
                     RVSTAGEAILARALLSDRQARGAVFVPIHWSDQYAGAARVGRLAPGLVDPHSGQPASK
                     NAAVRIERFAAARYGFALLRERPSNLDADYWAIAKCAGGWRLELAFARAERDWPAFAA
                     QLLQAGPGAQILAYRDLGAGQQRFAAFAGDCLAGALFLAAAPVAASRDWAVSQLEGKF
                     SSPAARLAIVAGRPPRGFADRGAVVCSCFGIGANQIASAIGAGCVSVEAIGDATRAGT
                     NCGSCRAEIRAMMGARALQAAE"
BASE COUNT         1197 a         1928 c         2062 g         1005 t
ORIGIN      
        1 gcgggaccgc gctttgagcg ccgccggctt cgatgtgaag cggcctgccg ttcctgaggc
       61 agcgcatgtc ccagagcgcc atgtcgatcg cgcagagcgc gagcgacgtg atcgcgccga
      121 ccgatgtggc gtgagtatgg aacaacaggt cgcgccagat cgcctcgatc atcgcggggt
      181 cgcggccgat caggcatggc gcaaggtggc gcgcaatcag atcgacgacg gcgtggccgc
      241 cggtgccgat cgtataggag tagccggtcc cgacgacgcc gtcgtcgccg aaaatgcgga
      301 cgatcggcgt ctcctggctg acaaaggcct ggattgcgtc ggtacgcttc accttcggcg
      361 gcaaatcgac catcaagact tcaacgcgat cgatcttagc catggcccgc ctccgcctgc
      421 gcgtcgccgc ctgaggaatc gacgccttcg tgaatgaagc tcatgatccg ccggctcatc
      481 gattcgtagc gccgctcgtt ctcgatctcg ccgaccaagg cgaaatcgcg catgaccagg
      541 atacggtcgg caagcgtgat catttccggc atatcgctcg aaatcagcaa cacggcgacg
      601 ccgcttcgcg tgatttcggc gatcagctca tggatcgcgg ttttcgtcct cacgtcgatg
      661 ccgacggtgg gctcgtcgat aatcaggata tcgacgccgg cggccagcca cttggcgatc
      721 gatacttttt gttgattgcc gccggaaagc ttgccgacct gctgttcgag cgatggcgtc
      781 ctgatgtcga gctttcgcgc caaaggcgcc gcggcgcccg cctcgcgccc cctggtgagg
      841 aggccgaaac gcgcggcgat ttgccgccat atcgtgatgg cgacattggc ccggacggaa
      901 tgggcgagga tcaggccctc gctcttgcga tcctcggaaa tatagccgat gcggtggcgc
      961 cgaacggcgt tggaaatgct ggaaatcgag acgggcgcgc cgttgagccg tatttcgccg
     1021 gcaagaatgt gcgcgtcccc gatcaaagcg cgggcgagct ccgtgcggcc ggagccgacc
     1081 aggccgtaga ggccgagtat ctctccccgg cgaagcgtga acgtgatgtc cttatggccg
     1141 agcgaagtgg cgacgctgcg cgcttcgagc gcccgcgcgt ccgagggctt tcgcgcgccg
     1201 atatcggcga cgcgctcctc gcggccgatc atcgccgaga cgatccggct gcggctcatc
     1261 gcggcgatcg gctcgccggc ggcggcgatt tttccgtcgc gcagcaccgt cacgcgatcg
     1321 gagatggcga tcacctcttc gagcttgtgg ctgacaaaga cgatcccctt gccttcgttc
     1381 ttcaggcggc gcagcaccgc gaagagcgct tgagcctcat ggtcggtgat tgacgcggtc
     1441 ggctcgtcga gcagcagcaa actcgcttcg agcgacagcg ccttggcgat ttccacgatc
     1501 tgcatctgcg cgaccgaaag gcggcgcacc tccgctttcg tatcgatcga cggatcgatc
     1561 agatcgagaa aacgccgcgc ctcggcatgc gccctgtcat agtcgacgag gccgcgccgg
     1621 atcggcaaat tctcgatcat gatattttcg ccgacggaaa agcgcggaat gaggttgcgc
     1681 tcctgatgga cggcgctgat gccggcggcg atggcgtcgc gcggcgatgc gaaagccgcc
     1741 ggccgatcgt cgatgagaag tcgtccgcgg tcgggccgat agagcccggt gatgattttg
     1801 atcagtgtcg acttgccggc cccgttttcc cccattaggg cgtggatctc gccgggacgg
     1861 acgtcaaagg agacgccgtc aagcgccacc acgccgggaa agttcttgcc gatgccgtcg
     1921 atcgcgaggc gcatgatttc agccgacctc gatacgggcc gcgcgcaagc gattgagccc
     1981 gaccgctgcg aggatcaacg cgccgagcag gaactgtacc cagtacggat cgactctggc
     2041 cagcaccatg ccgttctgga tgagcacgat cagcacgacc gccaaggcgg tcgccgcgac
     2101 ggaaatatgg ccgccggtca gcgccgcgcc gccgatgatc ggcgccgcga aagacatgac
     2161 cagccaatcg gcgccgatgg tcggctgagc cgagccgagt tgcgcctcgg ccaggatggc
     2221 gccgatcgcg gcgagcaggc ccgacagcgc gtgggcgagg acgatgatcc gatcgcgcgg
     2281 tatgccggaa agctccgccg catgcgggtt gccgccgacc gccagcagat agcggcccgg
     2341 cgcggtgcgg gcgaggaaca gagcgagcaa caaagccgcg atgaccggag ggatcaaaag
     2401 atacggcgcc gcgaagaagc gctcgacgcc gaaagcgacg aaagaccgcg gcaagttgta
     2461 gaagggaatc gattcggtca cgccgagatt gattccggcg aagaccgagg cggtcgccaa
     2521 tgtgatgatg aatccgttga tcccggtgcg gacggtcagc aagccgttca gcaggccgcc
     2581 gccaatcccg atcagaaggc ccagagcaac ggccgcgccg atcggaaagc cgagtttctc
     2641 catcatgccg ccgaccgcga tggcgacgag gccgccgagc gcgccaatcg ccagattgag
     2701 ctggccgacg gcgagcgtca gcatttgcgc gaaggcgacg acaagaacga cgcagaacga
     2761 gcgcagcatc acataaagat tgtattcggt gagaaaggcc ggcgatagga gcgagagagc
     2821 cgcaaagccg atcaggatgg cgacggcgag cgtcgaccat tcctgcgaga agaaacgccg
     2881 cgaaagtgtc atggccgtcg ccccaggctg cgccgctcgg cgaatacggt tcgatagcga
     2941 tcgagcagga cggcgatgag cagcatcacg ccgaggaaaa gctggagcca gaaattgccg
     3001 acctgcatga tcaacaggcc ggagcgaata gtcgtgacga gcagcgctcc aattatcgcg
     3061 ccgatcaccg aaatcgagcc gccgctgagc aacgcgccgc cgaggaccgg cgcgaggaac
     3121 gagggcagca gccaatcctg cccgatcgac ggcatggcgg cgccgagccg cgtcagcagc
     3181 atcaagccgg cggcggcggc gagaagaccg gaaagcgtgt gggcgatcac gatcacgcgg
     3241 ccaaccggca cgccggacag ctcggcggcg cggggattgg cgccggccgc gagaatctcg
     3301 cgacccaaag cggtgaagcg gaacagcacg atcagcgccg cgccgatcgc cagagcgatg
     3361 atcagcaagg gcgaaacgat tgcgaagcgc atccggccga acgccgcgat ggctggcggc
     3421 aggccgttga agatgaccgc ctttgtcagg atcagcatgc cgccggtaaa caagctggcg
     3481 ctggccaaag tgatgatgaa cgagttgacg ccggagcgca cgatcgcgaa accattggcc
     3541 cagccgagca gcgcgccgag caacagcccg gcggccaaag cgagcgggct cggcaatccc
     3601 catttctgca tcaaatagcc ggcgagcatg acgctgcaga cgccgatcga accaatcgcg
     3661 agattcatgc cgccggtcgc aaggacgacc atggtggaga agccgatgac gatgtcgatc
     3721 gccaaagtcc gcgacatcgc aaagagattg aaggacgaga cgaagcccgg cgcgaggctc
     3781 gcaaatacga tccagaacag gaggacaatc gcggcgaggc cgatcgcgcc cgattgctcg
     3841 gtcagccagg cgttgcgccc tgagcggaat tccgtatcga tcgagaggct catgccggtc
     3901 ttgcgctgtt gggagggcgc caagctgttc agccgcctgt cgccgcgtgg cgaagtcgct
     3961 tgtcgaggtg atggaaaagg ctccggaccg gcgcgtcctg cgcgggtccg gaacgcattg
     4021 ccgcggctta tttgcaatcc agatatttgg ttttcatcga gccgcgaatg tcgttggtga
     4081 ttttcttcag ctcatccttg taggagccga tattgtcggc gctgatcagc aacgtgcctg
     4141 aatcgataaa gcgcgcggtc tgcggcgtct tgatgaaggg cgcgtcggcc ttgaccttgc
     4201 atacgccgct cgccagcaga tcgaggacgt aagcgccgat atagccttgg ccgtagggat
     4261 tctgcgccat tgtgccggct gcgaatccgt ccttgatcgc gtcgagcacg atcttgtcgt
     4321 catcgatgcc gaccagcttg atgcgcttgt cgccgagatt gcgcagcgac ttggccgcca
     4381 ccaccgaggg gatataggcg gtggcgatca gcccgtcgat ctgatccttc tgcgctccga
     4441 gcagcgcgtt gatcttctgg tccgccgcct cctgcgaatc cgtatcggtg atgtgctgga
     4501 taagcgtcac cgcgccgccg gtctccttga ccgccttctc gacggcgtcc atgcgcaatt
     4561 tcgtattggg gtcgaccaaa agcccagcaa ggtggacgat cttgcccttg ccgcccatcg
     4621 ccttgatcag ggccttcgtg ccgagatagg cggaattgta gacatccgtg ccgaggcaga
     4681 aggccgcctt ggtcgggtcc tgagcgcagc cggcaagagc caaggccggc gcgttgttcg
     4741 cggcgagctc ctcgatcgtg gtgttgatgc cgaccgcgtc gccagggaaa ataccgaagc
     4801 cgttatagcc ttgcgtcaac aggctctcga tcagctccgt ctgcagattg agcttccatt
     4861 ccgtgggcac tttgaaatcg accgcggcga tgttgaagtc cttcttcgcg tcggcggcgg
     4921 cttgctccca gggcgcgaaa tagggatgcg gcccgccggg aatcaacgcg atcttgttgt
     4981 ttgaagcctc ggccgcgccg acgggcaggc agagaatggc ggccaatatg ggcaaagcgc
     5041 gaaacagcgc gcgaccggcg cttcttttca tcggtttcct cccttgagcg atcgcctggc
     5101 gcgtttctgg acaggctcgg cgacgatcat cgctagcaca aacgtatatc gcatgcaata
     5161 gacgttttct ggcgcaggcg cgaacgctcg atttgacgcg gaaaatcggc aaatcatcgg
     5221 ttcgaagagt tttgaggcgc aggctgtcag gcgcggcgaa gcgagccgaa ttcaggctcg
     5281 ggtctacgcc cgctttactc cgccgcttga agagcgcgcg cgcccatcat tgctcggatc
     5341 tcggcgcggc atgagccgca attcgtaccg gcccgggtgg cgtcgccgat cgcctcgacg
     5401 ctgacgcagc ctgcgccgat cgcgctcgcg atctgattag cgcctatccc gaagcatgag
     5461 cagacgactg cgccgcggtc cgcgaaaccg cgcggcgggc gcccggcgac aatcgcaagt
     5521 cgcgcggcgg ggctggagaa ttttccctca agttgggaga ccgcccaatc gcgcgacgcc
     5581 gcaaccggcg ccgccgcgag gaacagcgcg ccggccagac aatcgccggc aaaagcggcg
     5641 aagcgctgct ggcccgcgcc gagatcgcga taggcgagaa tctgcgcgcc agggccagct
     5701 tggagcagtt gcgccgcgaa tgccggccaa tcgcgctcgg ctcgggcgaa agcgagttcg
     5761 agccgccagc cgccggcgca tttggcgatg gcccaatagt cggcgtcgag attcgagggc
     5821 cgctcgcgaa ggagcgcgaa gccgtagcgc gcggcggcaa agcgctcgat gcggaccgcg
     5881 gcgttcttcg acgccggttg gccggaatga ggatcgacaa ggccgggcgc gagccggccg
     5941 acgcgcgccg ccccggcgta ttgatcgctc caatggatcg ggacgaacac agcgccgcgc
     6001 gcctgccggt ccgacagcaa ggcgcgcgcc aggatcgcct cgcccgccgt cgagacgcga
     6061 acaatatcgg ccccggcaat gccgaggcgc gcggcgtccg cgggatgaat ttcgatatac
     6121 ggttcggcca gatgttgcga taggcgcgcg cttttcccgg ttcgcgtcat ggtatgccaa
     6181 tgatcgcgca cg
//
LOCUS       DATGJW010000511        12279 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1447560, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000511 DATGJW010000000
VERSION     DATGJW010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 12279)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 12279)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..12279
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1447560"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..525
                     /locus_tag="VKU03_15510"
     CDS             <1..525
                     /locus_tag="VKU03_15510"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72724.1"
                     /translation="FVARQTLAPISNVSFSTKEQFDSSTLAMKRFDFITSATYGNLTA
                     NLDFGRYAAQPELGWLFSREGLLTSAKYKFADHWSVNGTVVFDMSRHFYDVVGQSTPV
                     FYAPTYGFGLGYSDECTTVNLSYTSTYTDPIASTPPLRDQTFLLQITLRTLGEVRAST
                     SVGQSSPETTTPTP"
     gene            556..1563
                     /gene="pdxA"
                     /locus_tag="VKU03_15515"
     CDS             556..1563
                     /gene="pdxA"
                     /locus_tag="VKU03_15515"
                     /EC_number="1.1.1.262"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011158611.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050570 - 4-hydroxythreonine-4-phosphate
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0042823 - pyridoxal phosphate biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-hydroxythreonine-4-phosphate dehydrogenase
                     PdxA"
                     /protein_id="HLJ72725.1"
                     /translation="MTVPAPNSPLALTMGDPAGIGPELALKAWLQRETVGAPFFVIAD
                     PRALSDLARKLSLDVPIIAVDPEGAAEVFDAALPVVPLSNSAAAEPGKPDPKFAAATL
                     ESIERAVAYTHAGQAAAIVTNPIAKKVLYDAGFAHPGHTEFLGELAARWGAPARPVMM
                     LWSPELAVVPVTIHIALADAPKALTREAILETVRIAARDLQMRFKIARPRLALAGLNP
                     HAGEGGAMGREEIEIIAPAIAELRREGLDVVGPLPADTMFHAAARKTYDVAIAMYHDQ
                     GLIPIKTLAFDEGVNVTLGLPFIRTSPDHGTAFDIAGTGRANVTSLIAALRLAARLAA
                     A"
     gene            1560..2465
                     /gene="rsmA"
                     /locus_tag="VKU03_15520"
     CDS             1560..2465
                     /gene="rsmA"
                     /locus_tag="VKU03_15520"
                     /EC_number="2.1.1.182"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018267516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000179 - rRNA
                     (adenine-N6,N6-)-dimethyltransferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA
                     (adenine(1518)-N(6)/adenine(1519)-N(6))-
                     dimethyltransferase RsmA"
                     /protein_id="HLJ72726.1"
                     /translation="MSLAQDGLPPLREVVAAFGLSAKKSLGQNFLFDLNLTGKIARAA
                     GPLENVAVVEIGPGPGGLTRALLAAGAKKVIAVERDARCIAALNAIGAHWPGRLAIIE
                     ADALSLDRSELLAHAEGAPIRICANLPYNIATALLVRWLESEPWPPFYERLTLMFQRE
                     VALRIVATPKERADYGRLAVLAGWRTEARILFDVPPAAFTPPPKVVSSVVALEPRAAP
                     APCDLRLLTAATQAAFGQRRKMLRQSLKSFAASLGVEVGALLKAAGIEETRRAEEVGV
                     QGFVALAEIAKRFTKPVNGAASDAP"
     gene            2514..3290
                     /locus_tag="VKU03_15525"
     CDS             2514..3290
                     /locus_tag="VKU03_15525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008188755.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72727.1"
                     /translation="MAEIQPPPALILKSLRKSFAGKTAVDNLNLQVRAGEFYALLGPN
                     GAGKTTTLRIVAGLLQPDQGEIEVFGVDARRQSLEAKRIIAWLPDEPMLYDKLDPLEY
                     LEFVAGLWSVEPKRAENLARELLETLGLWEHRRERCEGFSRGMKQKVALAGALIHEPK
                     LLMLDEPLTGLDAAVARQVKDLLVARVKNGATIILTTHILEVAERLADRIGIIQRGRL
                     IAEGTLQELRAGAARDQDKATLEDLFLDLTGEARETERAA"
     gene            3287..4816
                     /locus_tag="VKU03_15530"
     CDS             3287..4816
                     /locus_tag="VKU03_15530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72728.1"
                     /translation="MRLKSGTFLWFVTHDLRQHWRGLQGLFGGLSRGKVALLLVAAIL
                     TLHALAYPAALWINGGKGDALPAYMASGVLFILPWIIAQAMTATTRALYTRGDLDLVF
                     AAPVSARAVLAARAFAIATDGVASVAILLLPLANMNALIGHWRWLAIYPALAATGLFG
                     AGVGIVLALALFSACGPRRARLASQIVATLIGACFVLSLQALSMLPAATRAVFFAALA
                     PPAAFSFADPRALLWLPAMAAGGDARALALWCLVALAVFCIAALTFGKRFATAALLSA
                     GAPSARTSDRRGETFRASLGATLRAKERRLLWRDPWLMSQIMLQIIYTLPVSVILWRN
                     GGATGSVGVAFAPSIVVIAAQLAGSFAWIALSGEDAPEFLATAPVTRGEIERRKIESI
                     ALPIIIFLALPLAGLTWASPWSALCAAGFALGAGVSTALLNLWRQAPARRTMVLRRHS
                     QSKLVGLIEHFLSILWAVGAVIAIIGSWTAVVPVAIAIGALWLNRPRAPAVPALVPSR
                     A"
     gene            complement(4822..5466)
                     /gene="gmk"
                     /locus_tag="VKU03_15535"
     CDS             complement(4822..5466)
                     /gene="gmk"
                     /locus_tag="VKU03_15535"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008836047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004385 - guanylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HLJ72729.1"
                     /translation="MTAIMATKRRGLLLILSSPSGAGKTTLTRMLLQDKALDLTLSVS
                     VTTRERRSSEADGIHYHFITARQFETMRADGDLLESADVHGNRYGTPRAPVEAVLSEG
                     RDMLFDIDWQGAQQLREALGSDVVSIFILPPSMRELRARLDRRAEDSASQIAARLENA
                     RKEIARWRHYDYVLVNDDLQRAYGQIVAIIAAERLRRHRVEKGVDEFVTRLLSD"
     gene            complement(5463..6350)
                     /locus_tag="VKU03_15540"
     CDS             complement(5463..6350)
                     /locus_tag="VKU03_15540"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YicC/YloC family endoribonuclease"
                     /protein_id="HLJ72730.1"
                     /translation="MRLSSMTGFARASGAHGAFHWVFEIKTVNAKGLDLRLRTPPGFD
                     RIESEARIRLGKAIARGSCFATLTVQRESAGAEVKINAEALAALASAARKAAESARLA
                     PPTIDGLLAVRGIVDVAEGDEDEAAVAAACEGALKSLDEALAALVAMRQTEGAALAAF
                     LGERLTAIAALTKAADVNPGRKPEAIRARLAQSVAALMDTGRGFDENRLYQEAVLLAA
                     KADIREELDRLATHVAAARELMNAGGPVGRRLDFLAQELAREANTLCAKANDVALTAI
                     GLDLRSQIEQLREQIQNVE"
     gene            complement(6450..7475)
                     /locus_tag="VKU03_15545"
     CDS             complement(6450..7475)
                     /locus_tag="VKU03_15545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008130097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ72731.1"
                     /translation="MEYRRLGSSGLKVPALTFGAGTFGGKGEFFQAWGDTDAAGARRI
                     VDICLDAGVTMFDSADVYSSGESERVLGEGVKGRRDKVLISTKATFRAGEGPNDVGSS
                     RFHLINACEGSLKRLGTDYIDLFQLHGFDALTPVEEVLSTLDDLVRAGKIRYIGVSNF
                     SGWHLMKSLAVSDRYGYARYVANQTYYSLVGRQYEWELMPLGLDQGVGAVVWSPLGWG
                     RLTGKIRRGAKLPEVSRLHKTAANGPQMTDEYLYKVVDALDAVAAETGKSVPQIALNW
                     LLQRPTVSTVIVGARDEAQLKENLGAIGWSLSPEQIAKLDKASEEPLAYPYWHQRGFT
                     ERNPAPV"
     gene            7662..8672
                     /locus_tag="VKU03_15550"
                     /pseudo
     CDS             7662..8672
                     /locus_tag="VKU03_15550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018269705.1"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="SPFH domain-containing protein"
     gene            8686..9135
                     /locus_tag="VKU03_15555"
     CDS             8686..9135
                     /locus_tag="VKU03_15555"
                     /inference="COORDINATES: protein motif:HMM:NF014062.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NfeD family protein"
                     /protein_id="HLJ72732.1"
                     /translation="MAEFSALAHVEYFVWLIAGFVLCVAETVAPGAFLIWIGAAALLV
                     GAVDFVWPMELTLQLLVFAVAAVALVFVGRRVYGSLERSAPAPSLSRAHALIGKEFFL
                     DRAIERGFGAIRVDDSIWRVAGTEMAAGAKVRVVAVEDGSLLRVEKA"
     gene            complement(9156..9857)
                     /locus_tag="VKU03_15560"
     CDS             complement(9156..9857)
                     /locus_tag="VKU03_15560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011579813.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aquaporin"
                     /protein_id="HLJ72733.1"
                     /translation="MQKSIAEFIGTFTLVFFGCGAAVIGGMGTGATAINVLGIAFAFG
                     LAIVAMAYGIGPISGCHVNPAVSLGVFTAGRMSFSDLISYVVAQVAGAIVGALVLYLI
                     LSGKASGWNGGLGQDGWGAGYLGEYNALSAFIFEVVATFLFLVCILGVTQEGAPSHLA
                     GLAIGMTLVAIHIVGINVDGVSVNPARSIGPALVGVGTNPHAVAQLWLFIIAPLIGGA
                     LAGLLFANGTLTAKK"
     gene            10125..10475
                     /gene="yajC"
                     /locus_tag="VKU03_15565"
     CDS             10125..10475
                     /gene="yajC"
                     /locus_tag="VKU03_15565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006466672.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit YajC"
                     /protein_id="HLJ72734.1"
                     /translation="MFITPAFAQAAGAAAGPQDMLIQFAPIVVMIGIFYFLLLRPQQR
                     RAKEQRATIMAARRGDTIVTNGGLIGKITKAIDDNEVEVEVAPNVRVRIVRSGISDVR
                     AKGEPVKDAPSKPA"
     gene            10530..12119
                     /gene="secD"
                     /locus_tag="VKU03_15570"
     CDS             10530..12119
                     /gene="secD"
                     /locus_tag="VKU03_15570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018408105.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HLJ72735.1"
                     /translation="MLRFAPWRVASIIALVVAAILLIVPSFLSPATLTGLQKSLPSFL
                     PMRAIVLGLDLQGGAHVLLEVDSPSVTRAQVEALRDDVRGKLREARISIAGGIAMQPR
                     GVQVRITDDAGREKALPLLEQLNQPIGNAIIGASGHTLDYNVTSDGAIQVTLTDAAIN
                     DKVRRAVAQSIEVLRRRVDALGTTEPNIQQQGADRVLVEVPGLQDTTRLKEILGTTAK
                     LEFRLVADPGDPPNEVENLPQQSSPGTIPVQKRVMVQGEDLVDAQAAFDQNGAPDVNF
                     RFNLHGGQRFGQVTSENVGRPFAIVLDGKVISAPRILGPITGGSGVITGSFSVEEADN
                     LAVLLRAGALPAKLTVVEERTVGPGLGQDSIDAGKRAAFVGGVLVVVYMLTTYGIFGV
                     FADLALAVHILFIFASMALLGATLTLPGIAGVVFTIGMAVDSNVLIYERIREESHLGR
                     SVISALDAGFKRAFATIIDSNVTMFVAAVILYLFGSGPVRGFAVSLGLGILTSVVTAV
                     TMTRMMIALWYRSRRPTKLPI"
BASE COUNT         2080 a         4041 c         3863 g         2295 t
ORIGIN      
        1 ttcgtcgcgc gtcagacgct tgcgccaatt tcgaatgtct ccttctcgac gaaagagcag
       61 ttcgacagca gcaccttggc gatgaagcgg ttcgatttca tcaccagcgc gacctatggc
      121 aaccttaccg ccaatctcga cttcggccgc tacgcggcgc agcccgagct cggttggctg
      181 ttttcgcgcg aaggtctgct gaccagcgcc aaatataaat tcgccgatca ttggtcggtg
      241 aatggcaccg tggtcttcga catgtcgcgt catttctatg acgtcgtggg ccagtcgacg
      301 ccggtctttt acgcgccgac ctatggcttt ggtctcggct acagcgacga atgtacgact
      361 gtgaacttga gctacacgtc gacctatacc gacccgatcg cctcgacgcc gccgctgcgt
      421 gatcagacct tcttgttgca gatcacctta aggacgttgg gcgaggtgcg ggccagcacg
      481 agcgtcggcc agtcgtcgcc ggagacgacg acgccaacgc catgagcgcc gctcagtctc
      541 catcgttctc agactatgac cgtaccggcg ccgaatagcc cgctcgccct gaccatgggc
      601 gacccggcgg gcataggtcc tgaactcgca ttgaaagcat ggctgcaacg cgagactgtc
      661 ggcgcgcctt tttttgtcat cgccgacccc cgcgcgcttt ctgacctggc gcgcaaactg
      721 tcgctcgatg ttccgatcat cgcggtcgat cctgaaggcg cggccgaagt gttcgatgct
      781 gcattgccgg tggtcccgtt gtcgaacagc gcggcggctg agcctggcaa acctgatccg
      841 aaattcgccg ccgcgacgct ggaatcgatc gagcgcgccg tcgcttacac ccatgccggc
      901 caggcggcgg cgatcgtcac caatccgatc gcgaagaagg ttttgtacga cgcgggcttc
      961 gcgcatcccg gccataccga gtttctcggc gaacttgccg cgcgctgggg cgctccggcg
     1021 cggccggtga tgatgctatg gtcgccggaa cttgccgtcg tcccggtgac gatccatatc
     1081 gcccttgccg acgcgccaaa ggcgctgacg cgagaagcga tccttgagac cgtacggatc
     1141 gcggcgcgtg atttgcaaat gcgtttcaag attgcgcggc cgagactcgc tctcgccggc
     1201 cttaatccgc atgcgggcga agggggcgcc atgggccgcg aggagatcga gattatcgcg
     1261 cctgcgatcg ccgagcttcg ccgcgaaggg ctcgacgtcg tcggcccgct gccggccgat
     1321 acgatgttcc acgcggcggc gcgcaagacg tacgacgtcg cgatcgcgat gtatcacgac
     1381 cagggcctca tcccaataaa gacactggct ttcgacgaag gcgtcaatgt cacgctgggc
     1441 ctacccttca tccgtacctc gcccgatcac ggcacagcct tcgacatagc cgggaccgga
     1501 cgcgccaatg tcacgagctt gatcgccgcc ttgcgcttag cggcgcgtct tgccgcggca
     1561 tgagcctcgc gcaggatggc ctgccgccgc ttcgcgaagt cgtcgcggcc ttcggactga
     1621 gcgccaagaa gtccctcggg caaaattttc tcttcgatct gaacctcaca ggaaagatcg
     1681 cccgcgccgc aggccccctc gagaacgttg cggtcgtcga gatcggacct ggccccggcg
     1741 gcctcacccg cgcccttctc gccgccggcg cgaagaaggt catcgccgtc gagcgcgacg
     1801 cgcgctgcat cgcggctttg aacgcgatcg gcgcacattg gccaggacgg ctcgcgatta
     1861 tcgaagccga tgcgctgagc ctcgatcgca gcgaactcct ggcgcatgcc gagggcgccc
     1921 cgatccgtat ctgcgcgaac ctgccgtaca atatcgccac tgctcttctc gttcgctggc
     1981 tcgagagcga accttggccg cctttctacg aacggctgac gctgatgttc caacgcgaag
     2041 tcgcgcttcg catcgtcgcg acgcccaagg aacgcgccga ttacggccgt ctcgctgttc
     2101 ttgccggctg gcgtaccgaa gcgcgtatct tattcgacgt gccgccggca gcctttacgc
     2161 cgccgccgaa agtcgtctcc agcgtggtcg cgctcgagcc tcgcgcagcg cccgcgccat
     2221 gtgatcttcg cttgctgacg gccgcgaccc aggccgcctt cggccaaaga cgaaagatgc
     2281 tgcgccagtc gctcaaaagc ttcgccgcca gtctcggcgt cgaagtcggc gcattgctca
     2341 aggcggccgg aatcgaggaa acccgccgcg ccgaggaagt cggagtccag ggcttcgtcg
     2401 ctttggctga aatcgccaaa agattcacca aacctgttaa cggggccgca agcgacgcgc
     2461 cctaagactg cccgtaacag gccggcggct cgcgtcggcg cgtgagcgtc tccatggctg
     2521 aaatccaacc gccgccggct ctgatcctga agagccttcg caagagtttt gccggcaaaa
     2581 ccgccgtcga caatctcaat ctccaggttc gcgccggcga attctatgct ttgcttggcc
     2641 ccaatggcgc gggcaagacc acgactttgc ggatcgtcgc gggcctgttg cagcccgatc
     2701 agggcgagat cgaagtcttt ggcgtcgacg cgcgccggca atcgctcgag gccaaacgca
     2761 tcatcgcctg gcttccggac gagccgatgc tctacgacaa gctggatccg ctcgaatatc
     2821 tcgaattcgt cgccggcctt tggagcgtcg agccgaaacg cgccgaaaat ctcgcgcgcg
     2881 aacttctcga aacgctcggc ctttgggagc atcgccgcga acgctgcgaa ggtttctctc
     2941 gcggcatgaa gcagaaagtc gcgctggcgg gcgcgctgat tcacgagccg aaattgctga
     3001 tgctcgacga acctttgacc ggcctcgacg ccgccgtggc gcgccaggtc aaggatcttc
     3061 tcgttgcgcg ggtcaagaat ggcgcgacga tcattttgac gacgcatatt ctggaagtcg
     3121 ctgaacgcct cgccgaccgt atcggcatca tccaacgcgg gcgcttgatc gcggaaggaa
     3181 cgctgcagga attgcgtgca ggcgccgcgc gcgatcaaga caaagcaacg ctcgaagacc
     3241 tcttccttga tctcaccggc gaggcgcggg aaacggagcg cgctgcttga ggctgaaatc
     3301 cggcactttt ctctggttcg tcacacatga tctgcgccag cattggcgcg ggctgcaggg
     3361 cctctttggc ggtctgtcgc gcggcaaggt cgccttgctt ctcgttgcgg cgatcctgac
     3421 cttgcatgct ctcgcctatc ccgcggcgct ttggatcaat ggcggcaagg gcgatgcgct
     3481 cccggcctat atggcctcgg gcgtactttt catcctgccc tggatcatcg cccaggcgat
     3541 gaccgcgacg acgcgcgcgc tctatacgcg cggcgacctc gatcttgttt tcgccgcgcc
     3601 ggtttcggcg cgcgccgtac ttgcggcccg cgccttcgcc atagcgaccg acggcgtcgc
     3661 ctcggtcgct atccttcttt tgccgctcgc caatatgaat gcgctgatcg gccattggcg
     3721 ctggctcgcc atctatccgg ctttggcggc gacgggcctg ttcggcgccg gcgtcggcat
     3781 cgttctcgcg ctggcgctct tttccgcctg tggcccgcgc cgcgcccgcc tcgcctcgca
     3841 aatcgtcgcg acattgatcg gcgcctgctt cgtcctcagc ctgcaagcgc tgagcatgct
     3901 gcccgcggcg acccgcgccg tcttctttgc ggcgctcgcg ccgccggccg ccttcagctt
     3961 cgccgatccg cgcgccttgc tctggctgcc ggccatggcg gcgggcggcg acgcgcgcgc
     4021 gctggcgctg tggtgcctcg tcgcgctcgc ggtattttgc atcgcggctt tgacgtttgg
     4081 aaaacgcttt gcgacggcgg cgcttctctc cgccggcgcg ccatccgcac gtaccagcga
     4141 cagacgcggc gaaactttcc gcgccagttt gggcgcgact ttgcgcgcca aggagcgacg
     4201 gcttctttgg cgcgacccct ggctgatgtc gcagatcatg ttgcagatca tctatacgct
     4261 gccggtgagc gtgatcctct ggcgcaacgg cggcgcgacg gggtccgtcg gcgtcgcctt
     4321 cgcgccttcc atcgtcgtga tcgcggcgca gctcgccgga tccttcgcgt ggatcgcctt
     4381 gtccggcgag gacgcgccgg aatttcttgc gaccgcgccg gtcacccgcg gcgagatcga
     4441 acgccggaaa atcgaatcca tcgccttgcc gattattatc tttctcgcgc tgccgctcgc
     4501 cggccttacc tgggcttcgc cctggagcgc attatgcgcc gccggctttg cgctgggcgc
     4561 gggcgtctcg accgcgctgc tcaatctttg gcgccaggcg ccggcgcggc gcaccatggt
     4621 gctgcgacgc cattcgcaat cgaagctcgt cggcctgatc gagcatttct tgtcgattct
     4681 ttgggccgtc ggcgccgtca tcgccattat cggcagttgg acggcggtcg tgcctgtcgc
     4741 catcgcgatc ggggcgctct ggctcaaccg cccgcgcgcg cctgccgtgc cggcgctcgt
     4801 accgtcacgc gcttgaacgc ctcaatcgct taacaagcgc gtcacgaatt cgtccacgcc
     4861 cttttcgacg cggtgacggc gcagtcgctc cgcagcgatg atcgcgacga tctggccata
     4921 ggcgcgctgc aaatcgtcat tgacgagaac atagtcgtag tgacgccagc gcgcgatttc
     4981 cttgcgggcg ttctccaacc gggcggcgat ttgcgacgcg gaatcttcag cgcggcgatc
     5041 gaggcgcgct ctcagttcgc gcatcgacgg cggcagaatg aaaatgctga cgacatcgga
     5101 gccaagcgct tcgcgcaatt gctgcgcgcc ctgccagtcg atgtcgaaca gcatgtcgcg
     5161 cccttcggac aggacggctt cgaccggcgc gcgcggcgta ccgtagcgat tgccatgcac
     5221 gtcggccgat tccaagagat cgccgtcggc gcgcatcgtc tcgaattggc gcgcggtgat
     5281 gaaatgataa tggatgccgt cggcttccga ggagcggcgc tcgcgagtcg tcaccgatac
     5341 cgagagcgtg agatcgagcg ccttgtcctg cagcagcatg cgcgtcagcg tcgtcttgcc
     5401 ggcgccggag ggcgaggaaa gtatcagcag gaggccgcgt cgcttggtcg ccatgatcgc
     5461 cgtcattcga cgttttgaat ctgctcacgc aattgctcga tctgactgcg cagatcaagg
     5521 ccgatcgcgg tgagcgcgac atcgttggct ttggcgcaaa gcgtgttcgc ttcgcgggcc
     5581 aattcctgcg ccagaaaatc gaggcgccgt ccgacagggc cgccggcgtt catcaattcc
     5641 cgcgcggccg cgacatgggt ggcgagcctg tcgagctctt cgcgaatgtc cgccttggcg
     5701 gcaagtagga ccgcctcttg atacagacgg ttctcgtcaa agccgcggcc cgtatccata
     5761 agggcggcga ccgattgcgc aagacgggcg cggatggcct ccggcttgcg cccagggttt
     5821 acgtccgccg ccttggtcaa ggcggcgatc gccgtcaggc gctcgccgag aaaagccgcg
     5881 agcgccgcgc cttcggtctg gcgcatcgcg acgagggcgg cgagcgcttc gtccaggctc
     5941 ttcagcgcgc cctcgcaagc cgccgccacc gcggcctcgt cctcgtcgcc ctcggcgaca
     6001 tcgacgatcc cgcggaccgc caggagccca tcgatggtcg gtggggcgag gcgagcgctt
     6061 tccgccgcct tgcgtgccgc ggaggccagc gcggcgagcg cctcggcgtt gatcttgacc
     6121 tcagcgcccg cgctttcgcg ctggacggtc agggtcgcaa agcaagagcc gcgggcgatg
     6181 gccttgccga gacggatgcg ggcttcgctc tcgatccgat cgaagccagg gggcgtgcgc
     6241 agccgcaggt cgaggccctt ggcgttgacc gtcttgatct cgaagaccca atggaaagcg
     6301 ccatgagcgc cggacgctcg ggcgaagccc gtcatactgg aaaggcgcat cggctctgga
     6361 cgattcgaca gcggaaaggg ccggcaccat aggcgtcagc gccgccccgc tcaaggcgga
     6421 ggcggcgcgc aaggtcgggc gcggccgcct cacaccggcg ccgggttgcg ctccgtaaag
     6481 ccccgctggt gccagtaagg ataggcgagg ggctcctcgc tcgccttgtc gagcttggcg
     6541 atctgctccg gcgacaggga ccagccgatc gcgccgagat tttccttgag ctgcgcctcg
     6601 tcccgcgcgc cgacaatgac cgtcgaaaca gtcgggcgct gcagcagcca gttgagcgcg
     6661 atctgcggca cggacttgcc agtctcggcc gcgacggcgt cgagggcgtc gacgaccttg
     6721 tagagatatt catccgtcat ctgcggtccg ttcgccgctg tcttatgcag ccggctcacc
     6781 tcgggaagct tcgcgccgcg cctgatcttg ccggtgagac gtccccagcc gagcgggctc
     6841 catacgaccg cgccgacgcc ttgatcgaga ccgagcggca tcagctccca ttcgtactgc
     6901 cgcccgacca gcgaataata ggtctggttg gcgacatagc gcgcatagcc gtagcgatcg
     6961 gatacggcga gcgacttcat caaatgccag ccggagaaat tcgagacgcc gatataacga
     7021 atcttgccgg cgcgtacgag atcatcgagc gtgctgagga cttcctcgac cggcgtcagg
     7081 gcgtcgaaac cgtgcaactg gaagagatcg atataatccg taccgagccg cttcaacgaa
     7141 ccttcgcagg cgttgatcag gtggaagcgg gaggagccga cgtcgttcgg cccctcgccg
     7201 gcgcggaaag tcgccttggt cgaaatcaga actttgtcgc gccggccttt aaccccctcg
     7261 ccgagcaccc gctcggattc gccgctggaa tagacgtcgg cgctgtcgaa catcgtcacg
     7321 ccggcgtcga ggcaaatatc gacgattcgt cgcgcgccgg cggcgtctgt gtcgccccag
     7381 gcctggaaga attcgccttt gccgccgaac gtgccggcgc cgaaggtcag cgccggaacc
     7441 ttgaggccag acgaacccaa acgtcgatat tccatgtctt tgtcctttca cgaagattaa
     7501 gggaaacgag gcggcagatt ttcgagccgt tgggcggccg ttagaatcgg gggatgaccg
     7561 atcccgctcg atggcttttc gcaaggccgg gcggtaaatc aggaacatcc cgtaaatcgg
     7621 gctcgcccgt tggccgcggg cttttcggct gggaggcttt aatgccgatg ctcgcaggat
     7681 tcagcgtctt tgcgatcctt atcgctttgt tcgtcatcat cacgatcatg atgggcattc
     7741 ggcaggtgcc gcagggatat aattatacgg tcgagcgctt cggccgctac aacaagacgc
     7801 tgaccccagg cctcgccgtc atcatcccct atttcgatcg catcgggcgt aaattgaatg
     7861 tgatggagca ggtgatcgat gtgccgagcc aggagatcat caccaaggac aatgcatcgg
     7921 tgaaggtcga cggcgtcgcc ttcttccagg tgctcgacgc aaggcgcgct tcttacgaag
     7981 tctccaatct ggagcaagcg ctgctgaacc tcgtcatgac caatatccgc accgtcatgg
     8041 gctcgatgga tctcgatcaa ctgctgtctc atcgcgacga gatcaacaca aggctgctga
     8101 cggtaatcga cgccgcggcg cttccctggg gcgtcaaagt gacgcgcatc gagatcaagg
     8161 acatcgagcc gccgaaggat ctcatcgatt ccatggcccg gcagatgaag gccgaacgcg
     8221 agaagcgcgc cgtcatcctc gaggccgaag gcctgcgcca ggcggcggtg ctgcgcgccg
     8281 aaggcgccaa gcaggcggtg gtgctcgaag cggaaggccg caaggaggcc gccttccgcg
     8341 acgccgaggc gcgcgagcgt caggcccaag cctaagcgac cgcgaccatg gcggtgagcg
     8401 aggcgatcgc caatggcgat ctggcggcgc tgaactattt cgtcgccgaa aaatacgtgc
     8461 gggccataca ggctttggcg acggcgccga accaaaaaac attcatcatg ccgctcgacc
     8521 ttgcttcgct cgccggcacg ctcggcggca ttgcggagat tgcctcgagc gcgctcggcg
     8581 aaggcagagc gagcgacgcc gtccgccatt cgcgacctcc cgtaccgcca acgcggcccg
     8641 ccccgccgcc gatcgttcct ccaaccgctt gagaaaggcg cgccgatggc ggagttcagc
     8701 gcgctggctc atgtcgaata tttcgtctgg ctgatcgccg gcttcgtact ctgcgtcgcc
     8761 gagaccgtcg cgccgggcgc ctttctcatc tggatcggcg ccgccgcgct tctggtcggc
     8821 gccgtggatt tcgtctggcc gatggagctg acgcttcagc tcctcgtctt cgctgtcgcg
     8881 gcggtcgcgc tggttttcgt cggccgccgc gtttacggct ccctcgagcg ttccgccccg
     8941 gcgccgtccc tcagccgcgc ccatgccctg atcggcaagg aattcttcct cgatcgcgcg
     9001 atcgaacgcg gtttcggcgc gatccgagtc gacgacagca tctggcgcgt cgccggcacg
     9061 gaaatggccg ccggcgcaaa ggtgcgggtc gtcgctgtgg aggacgggag cttgctgcgg
     9121 gtggagaagg cgtgagctcc cctctccagt cccttctatt tcttcgctgt caacgtaccg
     9181 ttggcgaaca gcaggcccgc taaggcgccg ccgatcagcg gggcgataat gaagagccag
     9241 agctgcgcga cggcgtgcgg attggtcccg acgccgacga gcgcagggcc gatcgagcgc
     9301 gccggattga ccgagacgcc gtcgacattg atgccgacga tatggatggc gaccagggtc
     9361 atgccgatgg cgaggccggc gagatgggac ggcgcgcctt cctgcgtgac gccgaggatg
     9421 cagaccagga agaggaatgt cgcgaccacc tcgaagatga aggccgagag ggcattgtat
     9481 tcgccgagat agccggcgcc ccacccgtcc tggccgagcc cgccgttcca gcctgacgcc
     9541 ttgcccgaga ggatgagata aaggacgagc gcgccgacga tggcgccggc tacctgggcg
     9601 accacataag aaataaggtc gctgaagctc atccggccgg cggtgaaaac gccgaggctg
     9661 accgccggat tgacgtggca gccggaaatc ggaccgatcc cataggccat ggcgacgatc
     9721 gccaggccga aggcgaaagc gatgccgagc acgttgatcg ccgtagcgcc cgtgcccatg
     9781 ccgccgatca cggccgcgcc gcaaccgaaa aagacaagtg taaacgtgcc gatgaattca
     9841 gcgattgact tttgcatgtg tggccccctc gtcgaatatt gtttttatgc gccttattac
     9901 tcggctgacg ggtgattggc gattaaaaac gattgatttc ttctcggcgc atggctgggc
     9961 gccgaagagc gcgcgagcgc cgacggatcg tcttttcgac agggaagggg cgatggcggc
    10021 gcgaaagcgg ctacgccgca ttgctttcac ggcgaccgtc gctatattcc gcccgccaaa
    10081 agccagtcta agcgcgcttc gtcgcgtttt cggagttccg ttccttgttc attacgccag
    10141 cattcgccca ggccgcgggc gccgcagccg ggccgcagga tatgcttatt cagttcgcgc
    10201 ccatcgttgt gatgatcggc attttttatt tcctcctgct gaggccgcaa caacggcggg
    10261 ccaaggagca gcgggcgacc atcatggcgg cgcgccgggg tgacacgatc gtcaccaacg
    10321 gcggcctcat cggcaagatc accaaggcga tcgacgacaa cgaggtcgaa gtcgaagtcg
    10381 cgccgaatgt gcgcgtgcgc atcgtccgat ccggcatcag cgacgtgcgc gccaagggcg
    10441 aacccgtcaa agacgccccc agcaagccgg cgtgaccttt cgggcttcgt ttgtcgaaat
    10501 gagaatgcgc gtggagaccc actagtccaa tgcttcgctt cgccccatgg cgggtcgcct
    10561 cgatcatcgc gcttgtcgtg gccgcgatct tattgatcgt gccgagcttt ctgtcgccgg
    10621 caaccctgac cggccttcaa aagagcctgc cgtctttcct accgatgcgc gcgatcgttc
    10681 tcggccttga tttgcagggc ggcgcgcatg tcttgctgga ggtcgacagt ccttccgtca
    10741 cccgcgccca ggtcgaggct ttgcgcgacg acgtccgcgg caagctgcgg gaggcgcgca
    10801 tttccatcgc cggcggcatc gccatgcagc cacgcggcgt tcaggtgaga atcactgacg
    10861 atgccggccg cgagaaggcg ctgccgcttc tcgaacaatt gaaccagccg atcggcaacg
    10921 ccatcatcgg cgcctccggc catacgctcg attacaatgt gacgagcgac ggcgcgatcc
    10981 aggtgacgct gacggatgcg gcgatcaacg acaaggtgcg ccgcgccgtc gcgcaatcga
    11041 tcgaagtgtt gcgccgccgc gtcgatgcgc tcggcacgac ggagccgaat atccaacagc
    11101 aaggcgccga ccgcgtcctc gtcgaagtgc cgggtctaca ggacacgacg cggctgaagg
    11161 aaatcctcgg cacgaccgcc aagctcgaat tccgcctcgt cgccgatccc ggcgatccgc
    11221 cgaacgaagt ggagaacctg ccgcagcaaa gctcgcccgg cacaattccg gtgcagaagc
    11281 gcgtcatggt ccaaggcgaa gatctcgtcg acgcccaggc ggcttttgac caaaacggcg
    11341 cgcccgacgt caatttccgc ttcaatctcc acggcggcca gcgtttcggc caagtgacca
    11401 gcgaaaatgt cggacgtcct ttcgctatcg ttctcgacgg caaagtgatt tccgcgccgc
    11461 gcattctggg cccgatcact ggcggctcgg gcgtgatcac gggcagtttc tcggtcgagg
    11521 aggccgacaa tctcgccgtg ctgctgcgcg ccggcgcttt gcccgccaag ctcacggtgg
    11581 tcgaggagcg cacggttggt cccggtctcg gccaggattc gatcgatgcc ggaaaacgcg
    11641 ccgcgttcgt cggcggcgta ctcgtcgtgg tctatatgct gacgacttac ggcatattcg
    11701 gcgtcttcgc cgatctcgcg ctcgcggtgc atattctctt catcttcgcc tcgatggcgc
    11761 tgctcggcgc gacattgacg ttgcccggca tcgccggcgt ggtcttcacc atcggcatgg
    11821 cggtcgactc gaacgttctg atctatgaac gcatcaggga agaatcgcat ctcggccgtt
    11881 cggtgatctc ggctctcgac gccggcttta agcgcgcctt tgcgacgatc atcgactcga
    11941 acgtgaccat gttcgtcgcc gcggtcattc tctatctctt cggctcgggc ccggtacgcg
    12001 gcttcgccgt ctcgctcggg ctcggcattc tgacctcggt cgtcaccgcc gtgaccatga
    12061 cccgcatgat gatcgcgctc tggtaccgtt cgcgccgtcc gacgaagctg ccgatatgag
    12121 tgagccggcg tttctcctgc aagcgctgca ggccccgtcc cgtaccttcc cccgccgcgc
    12181 gggagagggg gccccaagcg ctgcgcctgt atctttcacg ccctgcgacg tggaatcgtt
    12241 atccacggcg tcgaggcgcc gctctcccgc tcggcgggg
//
LOCUS       DATGJW010000512         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_98023, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000512 DATGJW010000000
VERSION     DATGJW010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98023"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..159)
                     /locus_tag="VKU03_15575"
     tRNA            complement(83..159)
                     /locus_tag="VKU03_15575"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(123..125),aa:Arg,seq:ccg)
     gene            complement(230..1132)
                     /locus_tag="VKU03_15580"
     CDS             complement(230..1132)
                     /locus_tag="VKU03_15580"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012255508.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acyltransferase"
                     /protein_id="HLJ72736.1"
                     /translation="MELQRIGVAGGPARPGFPLRLPAKLVHDFCGLPASLARIGALEV
                     RLAATRKEIRQAQRLRYRVFFEEGGATPDPAARLIRRDVCPFDEVCDHLIAVDHAPAG
                     GGEPQVVGVYRLLRQDAAEANFGFYSAGEFDIAPMLARHGGKRFLELGRSCVAPGYRN
                     KPTLELLWRGVWTYARHHAVSAMFGCASLDGAEPRAHALALTYLSRAAQAPEAWRVAA
                     APGRGVAMAMLDEGALDARKAIRALPPLIKGYCRLGAHFGAEAVIDGKFGTTDVFVIL
                     PVAEIEQRYLAYFGTERAGAGLAA"
     gene            complement(1194..2966)
                     /locus_tag="VKU03_15585"
     CDS             complement(1194..2966)
                     /locus_tag="VKU03_15585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HLJ72737.1"
                     /translation="MDSTTLSMLLSARALAIAAGPGLALAFACALALAHLRRGAEMRA
                     AKAETANEALRDEVWRLRAEAEARERAEAASEAKSRFLATVSHEIRTPLNGILGMADL
                     LREAPLDPEHASYVEAIRSSGAALVRLIDEILDFSKIEAGRLALVSEWFDLHQLVEGV
                     VELLAPRAQSKGLEIAASIAADVPRLAFGDAMRLRQVLTNLAGNAVKFTERGGVGVAV
                     ARVADGRLRFEVADTGPGVPADRRAAIFEDFEQGDGSNARRHEGAGLGLAISRRIVGL
                     MGGDLSLADNPGGGSVFAFTVAVKAQERAKAQDDPPTPKLAGARALIIAHSPFEAPAI
                     AARLGEAGVAVRRAEGLDEGLAALAAGPQPDLVIVDCALGVEATNRLALAARAAGAPK
                     SLVLFSPFERRAFGETSLRGFDGWLVKPVRARSLFDRLAAEFPAAPAAKPAIAAPPAG
                     RGLLRALLAEDNDINATIAQKALRRLGFDVARAHDGEEAASLGGAAARGERPRFDVIL
                     MDIKMPGLDGCEATRAIRREERERGARPTPIIALTANAMDSDRRACLAAGIDEFLTKP
                     VDLAKLAETIDAVRLASPIRAALS"
BASE COUNT          489 a         1051 c         1102 g          438 t
ORIGIN      
        1 tggccagtcg atcggcccac cgaagattct gcaacaatat gtccgaaagc catcctctgg
       61 cagctaagac ccggggatga gttggcgcgc ccgaagggat tcgaacccct gacctctgcc
      121 ttcggagggc aacgctctat ccagctgagc tacgggcgcg ggcggccagc gaggcggcct
      181 tgcgtgctcc ttagccgatt tatcggaaat gcgaaacccg ttttgacgct caggcggcaa
      241 gtcccgcgcc ggcccgctcg gtcccgaaat aggcgaggta gcgctgctcg atttcggcga
      301 ccggcagaat gacgaagacg tcggtcgtgc cgaatttgcc gtcgataacg gcttctgcgc
      361 cgaaatgcgc gccgagccgg caatagcctt tgatcagcgg cggcaaggcg cggatcgcct
      421 tgcgcgcgtc gagcgcgcct tcatcgagca tcgccatggc gacgccgcgg ccgggcgccg
      481 cggcgacgcg ccacgcctcc ggcgcttgcg ccgcgcggct gagataggtc agcgccagcg
      541 catgcgcgcg gggctcggcg ccgtccaggc tggcgcagcc gaacatcgcc gaaacggcgt
      601 gatggcgcgc ataggtccaa acgccccgcc acagcaattc gagcgtcggc ttgttgcggt
      661 agcccggcgc gacgcaggag cggccgagtt cgagaaagcg cttgccgcca tggcgcgcca
      721 gcatcggcgc gatgtcgaat tcgccggcgc tgtaaaagcc gaaattcgcc tccgccgcgt
      781 cctggcgcaa caggcgatag acgccgacca cttgcggctc gccgccgccg gccggagcat
      841 ggtcgaccgc gatcaaatgg tcgcagactt cgtcgaacgg gcagacgtcc cggcggatca
      901 gccgcgcggc gggatcgggc gtcgcgccgc cttcttcgaa aaagacgcga tagcgcagcc
      961 tttgcgcctg gcggatttct ttccgggtcg cggcgaggcg gacttcgagc gcgccgatcc
     1021 tggcgaggct cgccggcagg ccgcaaaaat catggacgag cttcgccggc agccgcaacg
     1081 gaaagccggg ccgggcgggc ccgcctgcca cgccgatccg ctgcaactcc atgtcgatcg
     1141 cccccgattc gccccctttt tataaagggc ggaacgcggc cgtccgaatg aatttatgac
     1201 aaagcggcgc ggatcgggga ggcgaggcgg acggcgtcga tcgtctcggc gagcttggcg
     1261 agatcgaccg gctttgtcag gaactcgtcg atcccggcgg cgaggcaggc gcgccggtcc
     1321 gagtccatgg cgttggcggt caaggcgatg atcggggtcg gccgggcgcc gcgttcgcgc
     1381 tcttcgcgtc ggatcgcgcg cgtcgcctcg cagccgtcga ggccgggcat tttgatgtcc
     1441 atgaggatga cgtcgaaacg cggcctctcg ccgcgcgccg ccgctccgcc aaggctcgcc
     1501 gcctcctcgc cgtcatgcgc gcgcgccaca tcgaagccga gccgccgcag cgccttctgc
     1561 gcgatcgtgg cgttgatgtc gttgtcttcg gcgagcaaag cgcggagcag gccgcggcct
     1621 gcgggcggcg cggcgatcgc cggcttggcc gccggcgcgg cggggaactc cgccgccagc
     1681 cggtcgaaca gcgagcgggc cctgacgggc ttgaccagcc agccatcgaa gccgcggagg
     1741 gacgtctcgc caaaggcgcg ccgctcgaac ggcgagaaga ggacgaggct cttcggcgcg
     1801 ccggcggcgc gggcggcgag cgccaggcgg ttggtcgcct cgacgccgag cgcgcaatcg
     1861 acgatcacca ggtcgggctg cgggcccgcg gcgagcgccg ccaggccctc gtcgagtccc
     1921 tcggcgcggc gaaccgcgac gccggcttcg ccgagtcggg cggcgatggc cggggcttcg
     1981 aacggcgaat gggcgatgat cagggcgcgc gcgccggcga gcttcggcgt cggcggatcg
     2041 tcttgcgcct tcgcccgctc ctgcgctttg acggcgaccg tgaaagcgaa aaccgagccg
     2101 ccgccaggat tgtcggcgag gctcaggtcg ccccccatca ggccgacaat gcgccgagaa
     2161 atcgcaaggc cgaggccggc gccttcgtgg cgacgcgcgt tcgagccgtc gccctgctcg
     2221 aaatcctcga aaatcgcagc gcggcggtcg gccgggacgc cgggacccgt gtcggcgacc
     2281 tcgaagcgga ggcggccgtc cgcgacgcgc gcaacggcga cgccgacgcc gccccgctcg
     2341 gtgaatttca ccgcgttgcc ggcaagattg gtgaggactt ggcgcaaccg catggcgtcg
     2401 ccgaacgcca ggcgcgggac gtccgcagcg atcgaggcgg cgatttcgag gcccttgctc
     2461 tgggcgcgcg gggccagcag ttcgaccacg ccctcgacga gttggtgaag atcgaaccat
     2521 tcgctgacca gcgccagccg gccggcctcg attttcgaga aatcgaggat ttcatcgatc
     2581 aggcggacca gcgccgcgcc cgagctgcgg atcgcttcga cataactcgc atgttcggga
     2641 tcgagcggcg cctcgcgcaa gagatcggcc atgccgagaa tgccgttgag cggcgtgcgg
     2701 atttcgtggc tgacggtcgc caggaagcgc gatttcgcct cgctcgccgc ctcggcccgc
     2761 tcgcgcgcct cggcctcggc cctcagacgc cagacctcgt cgcgcaaggc ctcgttggcg
     2821 gtttcggcct tggcggcgcg catctcggcg ccccggcgca gatgcgccag ggcgagcgcg
     2881 caggcgaaag ccaaagcaag gccgggcccg gcggcgatgg ccaaagcgcg cgccgagagg
     2941 agcatcgaga gggtggttga atccatagcc gccaggcgtt gcgcgccgcc gagacggccg
     3001 gcgccgcgcg tcaatttgac agagcgcggt tgagaaacgg tttcttcgat ggatcaaagg
     3061 acgcgcagtc gcggcgccca
//
LOCUS       DATGJW010000513         6994 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_202069, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000513 DATGJW010000000
VERSION     DATGJW010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6994)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6994)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6994
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_202069"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2278)
                     /locus_tag="VKU03_15590"
     CDS             complement(<1..2278)
                     /locus_tag="VKU03_15590"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLJ72738.1"
                     /translation="MNAPVEARPGVGGADPQARASDPRLSAWVTANAGSGKTHVLVQR
                     VLRLLLEGTPPSRILCLTFTKSAAANMAGRIFKTLANWTSVDDEALAGEIEKSGAKRP
                     DAARLALARRLFARTIESPGGLKIETIHGFCGRLLRMFPFEANVAAGFRVVEEREAKH
                     LLNAAQESALADLARDPVAAARLRALATETGRQGLDALVGQALEMRAAIQEAVLHWGG
                     VAGYGEALRARLGLAPGEDSAALEEAILGGLGDRDAKLRIAGQLGEGSANDQQRAEAL
                     RAAALAPDRAAAVDAYFKIFFTREGKPRGGEAGKLVTNGVAKKYPPLDGLLRREQNRL
                     IGLCAKLKAARTAERSLGLILVAERILAAYARLKAERGLLDFDDLIERANALLERTDA
                     AWVLFKLDSGIDHILVDEAQDTSREQWRILGKIAEDFTAGKGARETRRTFFAVGDDKQ
                     SIFSFQGAAPDMFAEMRRLLQKRHVDADMPFAPVSLTLSYRSAKVVLDSVDAVFLVER
                     TWRGLTADNEAPPPHVAFRAYLPGVVEVWPPVGGEKEPLGSDWRLPLDAPANQDPPVL
                     LARRIAKVIADWLKPGSTARVHDPQTKKPRPIRPGDILILVRARGAFFEAMIRALKEA
                     HVRTLGADRLLLSDHIAVMDLLAAGKAALNADDDLSLAAVLKSPLFGFNDDDLIALAP
                     ERKAPLADALAESVEEKHRGAARRLALWRERAQALSPYDFYARLLGEEGGKRAMLGRL
                     GPEAGDAIEEFLSLALAFE"
     gene            complement(2275..5466)
                     /gene="addB"
                     /locus_tag="VKU03_15595"
     CDS             complement(2275..5466)
                     /gene="addB"
                     /locus_tag="VKU03_15595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000724 - double-strand break repair via
                     homologous recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="double-strand break repair protein AddB"
                     /protein_id="HLJ72739.1"
                     /translation="MSSARARLRTSLSILLFFREAALPDLRLFSIPAGAPFLASLARA
                     LLDGRLVEGFPSGSGPFALANATIYVPTRRAARALGEALRQASGAASLILPRIAPLGA
                     FEPTQDSRLFESPEAAREAPPAISALDRRMALARLTQAWGRALKGAIRAVDASGALVF
                     LASESPLVVAGPAQAFALAGDLAALIDDMTIEGVPWERLETLAPGEFDAYWRITLDFL
                     KIAAAHWPKHLEERGLIDEAARGARLVEDEIARLEGGGFGGPVIVAGSTGARRATAAL
                     IAAVARAPQGAVVLPDLDLALDEKSWAMIGGQQIATHPQAGLSRLLEAIGATRQDVRE
                     IGAVSAPLRARARLLSEALRPADSTELWRRRDDALAADKIGEALSGLSVIEAADEAEE
                     ALALAIAMREALETPGKTAALISPDLGLTRRVCAELARWDVVPDVSAGRALRESAAGV
                     FASLILAAAQSRQPLDLIALLDHPLTRLGRPRPVLDAAARALELGALRGVLPASGLDD
                     PQRLFAAARDTAKAGREDGARARLTERDWRSAEALLADILRSFAPLGALGENAPLAAH
                     IDAHRAALAAFSADEAGADRLTATADGAALAALLAEWRQAAPADFSMGAADYRALFEA
                     VAGETAAPEAGRPQSRLQVLGLLEARLLSFDLALLAGLDETVWPPAAETDAFLNRPMR
                     EQLGLSPPERRIGQTAHDLVAALGAPEAILSRAKKRGGAPTVASRFVQRLAAAAGAEA
                     FDAALARGERYLALARAIDRPAAFIRAERPRPAPPVRLRPTKLSVTRIETLRRDPYAI
                     YAERILKLKPLDPIAQPAGFREIGVEWHAALSEFTTAYPAGDLPAEARDRLMAIARAR
                     FADLLEDANFRLLNWPRIEQGLDFFLSFDRARRENLERIFSEEKGSLAIALGDGSMFT
                     LAAIADRIETTRWGAAFLVDYKTGAAPGVNEVRVGFAPQLTLEAAMMARGAFPNAPAQ
                     APVNAMYLKLGGAKGGENRGLNFKDEATLAEVAEEHYAALVAMLDQFRDEATPYLPRP
                     YPKFVGSFGVYDHLARVKEWSASGGLADEDSAP"
     gene            5627..6316
                     /locus_tag="VKU03_15600"
     CDS             5627..6316
                     /locus_tag="VKU03_15600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012454351.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008955 - peptidoglycan
                     glycosyltransferase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biosynthetic peptidoglycan transglycosylase"
                     /protein_id="HLJ72740.1"
                     /translation="MSKRRSFFSALARGAAWLVLALVCLFVLLVALYAFVPPVSTLML
                     ARLAEGKSYVRVYTPLNAIAPTVLAAVITSEDGSFCTNDGVDWGALREVLREEGKNGP
                     RRGASTITMQVAKNLFLWPSRSRIRKAIEIGMALVLGKVWSKRRTIEIYLNIAEWGDG
                     LFGVEAAAERYFDKHASQLSAGEAALLATALPNPIKRNPAHPRALQRRLAASLIARAR
                     VEGERVACLKR"
     gene            complement(6539..>6994)
                     /locus_tag="VKU03_15605"
     CDS             complement(6539..>6994)
                     /locus_tag="VKU03_15605"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007813329.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HLJ72741.1"
                     /translation="IEGHVASTKPLSYQGTLIDGIKVRFEAGRIVEAKATKGEEVLNK
                     VLDTDDGARRLGEVALVPHSSPISKSGLLFYNTLFDENAACHIALGQCYSKCFLDGAK
                     LSQEQIATRGGNKSLIHIDWMIGSAEIDIDGLDVDGGRTPVFRKGEWAA"
BASE COUNT         1051 a         2394 c         2468 g         1081 t
ORIGIN      
        1 gttcgaaggc cagagcgagc gacaggaatt cctcgatcgc gtcgcctgct tcggggccca
       61 ggcggccgag catggcgcgc ttgccgcctt cctcgccgag cagccgggcg tagaaatcgt
      121 acggggaaag cgcctgggcg cgttcgcgcc aaagcgccag ccggcgcgcc gccccgcgat
      181 gcttctcctc gacgctctcg gcgagcgcgt cggcgagcgg agctttgcgc tccggcgcga
      241 gggcgatgag atcgtcgtcg ttgaagccga aaagcggcga tttcagcacg gcggcgagcg
      301 aaagatcgtc gtcggcgttg agcgccgcct tgcctgccgc aaggaggtcc atcaccgcga
      361 tatggtcgct gagcagcagc cgatcggcgc ccagcgtccg cacatgggcc tctttcaagg
      421 cgcggatcat cgcttcgaaa aaagcgccgc gggcgcgcac caggatgaga atgtcgcccg
      481 gccggatcgg tcgcggcttt ttcgtctgcg gatcatgcac ccgcgccgtc gaacccggct
      541 tcagccagtc ggcgatgact ttggcgatgc ggcgcgcgag gaggaccggc ggatcctgat
      601 tggccggcgc atccagcggc aggcgccagt cggatccaag cggctccttt tcgccgccga
      661 ccggcggcca gacttcgacc acgcccggca gataggcgcg aaacgcgaca tgcggcggcg
      721 gcgcttcatt gtcggcggtg aggccgcgcc atgtcctctc gaccaggaac acggcgtcga
      781 cgctgtcgag cacgactttc gcggagcggt acgaaagcgt cagcgagacc ggcgcgaacg
      841 gcatatcggc gtcgacatgg cgcttctgga ggagccggcg catctcggcg aacatatcgg
      901 gcgcggcgcc ctggaacgag aagatcgact gcttgtcgtc gccgaccgcg aagaacgtgc
      961 gccgggtctc ccgcgcgcct ttgcccgcgg tgaaatcctc ggcgatcttc ccgaggattc
     1021 gccattgctc gcgcgacgta tcctgcgctt cgtcgacgag gatgtggtcg atgccggaat
     1081 cgagcttgaa gagaacccag gccgcgtcgg tccgctccag aagcgcattc gcccgctcga
     1141 tcagatcgtc gaaatcgagc aggccgcgct cggctttcag ccgggcgtag gcggcaagga
     1201 tgcgctcggc gacgaggatc aggccgaggc tgcgctcggc ggttcgcgcc gccttcagct
     1261 tcgcgcagag cccgatcagg cgattctgct cgcgccgcaa caggccgtcc agcggcggat
     1321 atttcttggc gacgccgttg gtgaccagct tgccggcttc gccgccgcgc ggcttgccct
     1381 cgcgggtgaa gaaaatcttg aaatatgcgt cgaccgcggc ggcgcgatcc ggggcaagcg
     1441 cggccgcgcg caaggcctcg gcgcgctgtt ggtcgttcgc cgagccttcg ccgagctggc
     1501 cggcgatccg cagcttcgcg tcgcggtcgc cgaggccgcc gaggatcgcc tcttcaagcg
     1561 cggccgagtc ctcgcccggc gcaaggccga gccgggcgcg cagcgcttcg ccataaccgg
     1621 cgacgccgcc ccaatggagg accgcctcct gaatggcggc gcgcatttcc agtgcttggc
     1681 cgaccaacgc gtcgagccct tgccggccgg tctccgtcgc cagggcgcgc agcctcgccg
     1741 cggcgaccgg gtcgcgcgcc aggtcggcga gcgcgctttc ttgcgccgcg ttcagcaagt
     1801 gcttggcttc gcgctcctcg acgacgcgaa agccggccgc gacattcgct tcgaacggga
     1861 acatgcgcag caggcggccg caaaagccgt ggatcgtctc gattttcagg ccgcctggcg
     1921 attcgatcgt gcgggcgaaa agccgccgcg cgagcgccag ccgggcggcg tccggccgct
     1981 tggccccgga tttctcgatc tcgccggcca aggcctcgtc gtcgacgctc gtccaattcg
     2041 ccagcgtctt gaagatgcgg ccggccatgt tggccgccgc gcttttggtg aaggtcaggc
     2101 agaggatgcg cgacggcggc gttccttcaa gcaacagccg cagcacgcgc tgcacgagaa
     2161 cgtgggtctt gccggagccg gcattggcgg tgacccaggc cgagaggcgc ggatcggaag
     2221 cgcgcgcctg cggatcggcg ccgccgacgc ccggcctcgc ttcgaccggc gcgttcacgg
     2281 ggcgctgtcc tcgtccgcga gcccgcccga ggccgaccat tccttgactc gcgccaagtg
     2341 atcgtagacg ccgaaagagc cgacgaattt cggatagggc cgcggcaggt agggcgtcgc
     2401 ctcgtctctg aactgatcga gcatcgcgac caaggcggcg tagtgctcct cggcgacctc
     2461 ggccaaggtc gcctcgtcct tgaaattcag accgcggttt tcgccgccct tggcgccgcc
     2521 gagcttgagg tacatcgcgt tcaccggcgc ctgggccggc gcgttcggaa aggcgccgcg
     2581 cgccatcatc gcggcttcca gcgtcaattg cggcgcaaag ccgaccctca cctcgttgac
     2641 tcccggcgcg gcgccggtct tgtagtcgac gagaaacgcc gcgccccagc gcgtcgtctc
     2701 gatgcggtcg gcgatcgcgg cgagcgtgaa catggagccg tcgccgagcg cgatggcgag
     2761 gctgcccttc tcctcggaga agatgcgctc gaggttttcg cgccttgcgc ggtcgaaact
     2821 caggaaaaag tccagcccct gttcgatgcg cggccaattc aacagacgga aattcgcgtc
     2881 ttcgagcagg tcggcgaatc gcgcccgggc gatcgccatg agccggtcgc gcgcttccgc
     2941 gggcaggtcg ccggccggat aggccgtcgt gaattcgctc agcgcggcgt gccattcgac
     3001 gccgatctcc cggaagccgg ccggctgggc gatcgggtcg agcggcttca gcttcagaat
     3061 gcgctcggcg tagatcgcgt aaggatcgcg gcgcagcgtc tcgatgcggg tgacgctgag
     3121 cttggtcggc cgcagccgga cgggcggcgc cgggcgcggc ctttcggcgc ggatgaaggc
     3181 ggccggccgg tcgatggcgc gggccagagc gagatagcgt tcgccgcgcg ccagagccgc
     3241 gtcgaacgcc tcggcgccgg cggcggccgc gaggcgctgc acgaagcgcg aagcgacggt
     3301 cggcgcgccg ccgcgcttct tggcgcgcga gagaatggct tcaggggcgc cgagcgcggc
     3361 gacgaggtca tgcgcggtct ggccgatgcg tcgctcgggc ggcgagaggc cgagctgctc
     3421 gcgcatcggc cggttgagga aggcgtcggt ctccgcggcc ggcggccaga ccgtctcgtc
     3481 gaggccggcg agcaaagcaa gatcgaacga cagcagccgc gcttcgagca ggccgaggac
     3541 ctgcagccgg ctttgcggcc gccccgcctc cggcgccgcc gtttcgccgg cgaccgcttc
     3601 gaaaagcgcg cggtaatcgg ctgcgcccat gctgaaatca gccggcgcgg cttgccgcca
     3661 ctcggcgagc agggcggcga gcgccgcccc gtcggcggtc gcggtcagcc gatcggcgcc
     3721 ggcttcgtcg gcgctgaacg ccgcgagcgc cgcgcggtgc gcgtcgatat gcgccgcgag
     3781 cggcgcattc tcgccgagcg cgccgagcgg cgcgaagctg cgcaaaatgt cggcgagcag
     3841 cgcttcggcg ctgcgccaat cccgttcggt caggcgcgcc cgcgcgccgt cttcgcgacc
     3901 ggctttcgcc gtgtcgcgcg ccgccgcgaa gaggcgctgc ggatcgtcga ggccgctcgc
     3961 cggcaagacg ccgcgcagcg cgccgagctc cagcgctcgc gccgccgcgt cgaggaccgg
     4021 ccgcggccgg ccgagccggg tcaggggatg gtcgagcaag gcgatcagat cgagcggctg
     4081 gcgcgactgg gccgcggcga ggatgaggct ggcgaaaacg ccggcggcgc tttcgcgcaa
     4141 ggcgcggccg gccgagacgt cggggacgac gtcccagcgc gcgagctcgg cgcagacccg
     4201 ccgcgtcaag ccgagatcgg gggagatcaa agcggcggtc ttgccgggcg tctccaaggc
     4261 ttcgcgcatg gcgatggcga gggcgagcgc ctcttcggct tcgtccgccg cttcgatcac
     4321 gctcaaaccg gacagagcct cgccgatctt gtcggcggcc agcgcatcgt cgcggcgccg
     4381 ccagagctcg gtcgaatcgg ccggccgcag cgcttcgctg aggaggcgcg cgcgcgcccg
     4441 aagcggggcg gagacggcgc cgatctcgcg gacgtcctgg cgcgtcgcgc cgatcgcttc
     4501 gagcaggcgc gacaatcccg cctgcggatg cgtcgcgatc tgctgtccgc cgatcatcgc
     4561 ccaggatttt tcgtccagcg cgagatcgag gtccggcagc accaccgcgc cttgcggggc
     4621 gcgggcgacc gccgcgatca gcgccgccgt cgcgcgccgc gcgccggtcg agccggcgac
     4681 gatgaccggg ccgccgaacc cgcctccttc gagacgggcg atctcgtcct cgaccagccg
     4741 ggcgccgcgc gccgcctcat cgatcaggcc gcgctcttcg agatgtttcg gccaatgcgc
     4801 ggcggcgatt ttgaggaaat cgagcgtgat gcgccaatag gcgtcgaatt cgccgggcgc
     4861 gagcgtttcg agccgctccc agggaacgcc ctcgatcgtc atgtcgtcga tcagggcggc
     4921 gagatcgccg gccaaggcga aggcctgggc cggaccggcg acgacgagcg gcgattcgct
     4981 cgccaggaag accagcgcgc cgctcgcgtc gacggcgcgg atcgcgcctt tgagcgcgcg
     5041 gccccaggcc tgcgtcaggc gggccagcgc catacgccga tcaagcgcgg agatcgccgg
     5101 cggcgcctcg cgcgcggcct ccgggctctc gaacaggcgg ctgtcctggg tcggctcgaa
     5161 cgcgccgagc ggggcgatgc gcggcaggat caggctcgcg gcgccgctcg cttgccgtag
     5221 cgcttcgccg agcgcgcgcg ccgcgcgccg cgtcggcaca tagatcgtcg cgttggccaa
     5281 agcgaatgga ccgctgccgc tggggaagcc ttcgaccaag cgcccatcca gcagcgcgcg
     5341 ggccaagctc gcgagaaacg gcgcgccggc gggaatcgaa aaaagtcgca gatcgggcag
     5401 cgcggcctcg cgaaaaaaga gcaggatgga aagcgatgtt ctgagccggg cgcgcgccga
     5461 tgacaagcgg ggcgcgcgat tcgaaacgcg ttggtcccag gattttccgt tttgacgatt
     5521 cggcaaacag gagcgcgccg cggcccgcgc tcctccgcgc ttcgctgttt ccgattgccg
     5581 gaggtcgagc gcttgcctct ccgcctcggc ttggccaaac tgccgcatga gcaagcggcg
     5641 ctcgttcttt tctgctttag cgcgcggggc ggcgtggctt gtcctcgcgc ttgtctgtct
     5701 ttttgtcctt ctcgtcgcgc tctacgcctt cgtgccgccg gtctcgacgc tgatgctcgc
     5761 ccggctcgcc gaaggcaaga gctacgtgcg ggtttacacg ccgctcaacg cgatcgcgcc
     5821 gaccgtcttg gccgcggtga tcacgtcgga ggacggcagc ttctgcacga atgacggcgt
     5881 cgattggggc gctttgcgtg aagtgctgcg ggaagaaggc aagaacgggc cgcgccgcgg
     5941 cgcttcgacg atcaccatgc aagtcgcgaa gaaccttttt ctgtggccca gccgttcgag
     6001 gatccgcaag gcgatcgaga tcggcatggc gctggtcctc ggcaaggtct ggtccaagcg
     6061 ccgcaccatc gaaatctatc tcaacatcgc cgaatggggc gacggcctgt tcggcgtcga
     6121 ggcggcggcc gagcgctact tcgacaagca tgcaagccaa ctcagcgccg gcgaggcggc
     6181 gctgctggcg accgcgctgc ccaacccgat caagcgcaac ccagctcatc cgcgcgcgct
     6241 ccagcgccgt ttggcggcga gcctgatcgc gcgggcgcgc gtagaaggcg agcgcgtggc
     6301 ttgcttgaaa cggtgagagt cgtcttggcg tcgatccgag cgcagcgagg gtacggaagc
     6361 agctcattcg gagcgcggcg cctttcctcc cccgcgaaag cgggggaggg ggaccatgcg
     6421 aagcatggtg gagggggcct cggtctatca ggataaaccc cagccgccgc ggctcccacc
     6481 gcgctatcgc gcgttccccc tcccccgttt cgcttacgct tcacggggga ggatgggcct
     6541 aggccgccca ctcccccttc ctaaacaccg gcgtccttcc gccatccacg tcgagcccat
     6601 caatatcgat ctcggcggag ccgatcatcc aatcgatgtg gatcaggctc ttgttgcctc
     6661 cgcgcgttgc gatttgctct tggctcagct tggcgccgtc aaggaagcat ttcgaatagc
     6721 attggccgag cgcgatgtgg caggcggcgt tctcgtcgaa caacgtattg tagaatagta
     6781 ggccgctctt cgagatcggc gatgaatgcg gcaccagcgc gacttcgccg aggcggcgcg
     6841 cgccatcgtc ggtatcgagc actttgttca gcacttcctc gcctttcgtc gccttggcct
     6901 cgacgatccg ccctgcttcg aaacgcacct tgatgccgtc gatcagcgtg ccttggtacg
     6961 acagcggctt ggtgctggcg acatgaccct cgat
//
LOCUS       DATGJW010000514         7445 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_927923, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000514 DATGJW010000000
VERSION     DATGJW010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7445)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7445)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7445
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_927923"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1066)
                     /locus_tag="VKU03_15610"
     CDS             complement(<1..1066)
                     /locus_tag="VKU03_15610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018043500.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RNA degradosome polyphosphate kinase"
                     /protein_id="HLJ72742.1"
                     /translation="MVAPAPGDPEAPPSVADATQVDSLRNPARFINRELSWLEFNRRV
                     LEEACNRNHPLLEQLRFLSISADNLDEFFMVRVAGLIDQVASGLLAPSEDGLTPADQL
                     VRIGERVADLVRAQQTRWRELRAELASAGVVIVDVGDLADLEKRWLEDYFLLHVFPVL
                     TPLAIDPAHPFPFIPNLGFSLAMQLVRRSDQRAMSALVRVPGKIDRFVKLPDLAGGVQ
                     RFVMLEQIIVAFTHQLFPGYEVVGQGCFRIIRDSDIEVEEEAEDLVRQYESALKRRRR
                     GSVIRLEIDAAMPEPLRALVAADLDIVADGVFLVEGMLALNELSQLVSVDRPDLKYSA
                     YNPRFPERVRENGGDCFAAIR"
     gene            complement(1256..1939)
                     /locus_tag="VKU03_15615"
     CDS             complement(1256..1939)
                     /locus_tag="VKU03_15615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015931992.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA/Hda family protein"
                     /protein_id="HLJ72743.1"
                     /translation="MTGLARQLILDLPHEPRYSRDDFLAAPSNVEALRAIDAWPDWPG
                     RMLLLVGPAGSGKSHLGAIWAERAGASKLKGEALGEADLPQLAVAKALLIDDADRLGA
                     AEAALFHLVNLARERGASLLLTANAAPDLWGLEKADLLSRLRLAPLAALREPDEALAR
                     AVLVKLFDDRQLRVEPAVIDYIALRIERSLAAARAIVAALDREALARGRAVTRPMAAA
                     LLKDGETER"
     gene            complement(1995..2387)
                     /locus_tag="VKU03_15620"
     CDS             complement(1995..2387)
                     /locus_tag="VKU03_15620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018321186.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ72744.1"
                     /translation="MSQTIGALTFVVPDYDEAIAYFTETLGFGLVEDKPLAPGKRWVL
                     VAPPGGGARLLLAKAADDRQAARIGDQTGGRVFLFLHTDDFWRDHRRLLAKGVRFVEA
                     PRDESYGWVAVFEDRWGNRWDLLQPRRR"
     gene            complement(2384..2947)
                     /locus_tag="VKU03_15625"
     CDS             complement(2384..2947)
                     /locus_tag="VKU03_15625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176047.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HLJ72745.1"
                     /translation="MQFDKFFASLPNLITLARLLLAPLAVLMIVSQRFLPAFAIFVIA
                     GLSDGVDGFIAKRFDLRTELGAYLDPLADKALLVSIYVTLAVVGELPAAIAIIVVSRD
                     LMILIAVLISWLLDNPVAIRPVWVSKFNTVAQIAVAALVLAAKAFGFAPLPGEEFAVW
                     LVGASTIASGAVYVAQWLEHMNKGAAT"
     gene            3146..3604
                     /locus_tag="VKU03_15630"
     CDS             3146..3604
                     /locus_tag="VKU03_15630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72746.1"
                     /translation="MTDEAPDPAMHYGNLISAYTEAIRLSDFKANLTVLFVAIMMGPV
                     VASKDKFPHFLSLQIALAPFLVAFFCLLMCVFPRFPRRGRRNFLVSRNPQRGDFLYSE
                     HREEDVGQLKLRCAILSGILFWKTLFLKISFFICLATLIATFLLLTYSLL"
     gene            3620..4069
                     /locus_tag="VKU03_15635"
     CDS             3620..4069
                     /locus_tag="VKU03_15635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72747.1"
                     /translation="MASDAKTYYRNQIAAYTDTIRISDFKANVAIIYGAFTIGPVMGF
                     ADKFPPFLPLNAVLLPFVVVFFCLLVCLFPRYPRAGRETFLIKANAKPEDFVVPANQQ
                     VVLEGQQTLCVILCNILYWKTIMLQISFAIYILGTLLAAALLAYFKL"
     gene            4232..4627
                     /locus_tag="VKU03_15640"
     CDS             4232..4627
                     /locus_tag="VKU03_15640"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010686591.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3307 domain-containing protein"
                     /protein_id="HLJ72748.1"
                     /translation="MASLSPTVPLGSFCALLLALAVKHFVADFLFQTHWMAMGKERCE
                     GWLAPLAAHVLCHAVLTLCIALVVAPRLWWLALVDLAIHTCVDRCKSIVGHRSGWNMT
                     KPQYWWLLGFDQFLHQITNIGLAAAFYLL"
     gene            4686..5756
                     /gene="purM"
                     /locus_tag="VKU03_15645"
     CDS             4686..5756
                     /gene="purM"
                     /locus_tag="VKU03_15645"
                     /EC_number="6.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012317498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004641 -
                     phosphoribosylformylglycinamidine cyclo-ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylformylglycinamidine cyclo-ligase"
                     /protein_id="HLJ72749.1"
                     /translation="MTDAPRPSLTYAEAGVDIDAGAAMVEAIKPLVRATRRPGADAEI
                     GGFGGLFDLKAAGFADPVLVAANDGVGTKVKIAIDCAIHDTIGIDLVAMCVNDLVVQG
                     AEPLFFLDYYATGRLDPAVGAAVVKGIAAGCREAGCALIGGETAEMPGLYAGRDYDLA
                     GFAVGAAERGRLLPKGGLKPGDIALGLASSGVHSNGFSLVRRIVERRALPFDAPAPFA
                     PGLSLGQALLAPTRIYVKPLLQALAASPAILALAHITGGGFPDNLPRILPDGLGVAVD
                     LGAFAPPPVFGWLAEAGGLAQSEMLRTFNCGIGMVAFAAAEGAAEAAAALAAAGLAPV
                     QIGEVVAAASGPRVRFSGRLVL"
     gene            5756..6364
                     /gene="purN"
                     /locus_tag="VKU03_15650"
     CDS             5756..6364
                     /gene="purN"
                     /locus_tag="VKU03_15650"
                     /EC_number="2.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004644 - phosphoribosylglycinamide
                     formyltransferase activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoribosylglycinamide formyltransferase"
                     /protein_id="HLJ72750.1"
                     /translation="MLRKRTAILISGRGSNMAALIAAARAPDFPAEIVLVLSNKPDAE
                     GLARAESAGIAVAAVDHKIYAGRDEFEASMQALLDIHRVELICLAGFMRVLSPPFVTR
                     WRGRMLNIHPSLLPAFRGLDTHRRALDAGVKIHGCTVHFVEPELDSGPIVAQSFVSVC
                     DDDTPETLAARVIEKEHALYAEALALVAAGRITLESGVAPKS"
     gene            6521..6799
                     /locus_tag="VKU03_15655"
     CDS             6521..6799
                     /locus_tag="VKU03_15655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72751.1"
                     /translation="MRQMVSLSLCASIVIGSCGAAAAADVSTWRWKTSQALREQQDRE
                     ADAAKVYREASAFEVTPGMQQRGDPAAGADPLYSGDLKAAVEAQRSGQ"
     gene            complement(6808..>7445)
                     /locus_tag="VKU03_15660"
     CDS             complement(6808..>7445)
                     /locus_tag="VKU03_15660"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HLJ72752.1"
                     /translation="ASEIGAELKRHGFAPQILQDKRDALRAAFAEDAAVIVLDRLLLG
                     EDSLELVETMRKRGVVAPIVIISASESAEERIRGLRAGADDYLVKPFALGELMARIDA
                     IARRLGDNRATRLKAGPLEMDLIEQSVRRDGRPIELLPREFKILEYFMRHPGEVVTRE
                     ALLEHVWRYRSVRLTNTVDVHLSNLRRKLDVGADRPLIVNKRRAGFVLRTD"
BASE COUNT         1273 a         2516 c         2369 g         1287 t
ORIGIN      
        1 ggcggatcgc cgcgaagcaa tcgccgccgt tctcgcggac gcgctcggga aagcgcgggt
       61 tgtaggcgga atatttgagg tccggccgat cgacggagac gagctgcgac aattcgttga
      121 gcgccagcat gccttcgacg aggaacacgc cgtcggcgac aatgtcgaga tcggccgcga
      181 cgagggcgcg cagcggctcc ggcatcgccg catcgatttc gagccggatg accgagccgc
      241 ggcggcggcg cttcagcgcg ctctcgtatt gccgcaccag gtcttcggcc tcttcctcca
      301 cttcgatgtc gctgtcgcga atgatgcgga agcagccctg cccgaccact tcatagccgg
      361 ggaacaattg gtgcgtgaat gcgacgatga tctgttcgag catcacgaag cgctgcacgc
      421 cgccggcgag atcaggcagc ttgacgaaac ggtcgatttt gcccggcacg cgaacgaggg
      481 cgctcatcgc gcgctgatcg ctgcggcgaa cgagctgcat cgccaggctg aagccgagat
      541 tgggaatgaa cggaaacgga tgggccgggt cgatcgccag cggcgtcaag accgggaaaa
      601 cgtgcaggag aaaataatcc tccagccagc gcttttcgag atcggccagg tcgccgacat
      661 cgacgatgac caccccagcg ctcgccaatt ctgcgcgcaa ttcgcgccag cgcgtctgct
      721 gcgctcggac gagatcggcg acccgctcgc cgatgcggac cagttggtcg gccggcgtca
      781 gcccgtcctc ggacggcgcc agcaggccgg aggccacctg atcgatcagg ccggcgaccc
      841 gcaccatgaa gaattcgtca agattgtcgg ccgagatcga caggaaccgc aattgctcga
      901 gcagcggatg gttgcggttg cacgcctctt ccaaaacgcg acgattgaat tcgagccacg
      961 acaattcgcg attgatgaag cgcgccggat tgcgcaggct atcgacttgc gtcgcgtcgg
     1021 cgaccgaagg cggcgcctcg ggatcgccgg gcgccggcgc gaccacgacg agcggcgtct
     1081 cgatctgcgg gttggcttga gcccgcggat cctcaatttg cggcgccgcg gcggcgggac
     1141 gacgcgccaa aaacaagcct ccacgaaaag aaccaatcgg cgcgccggtt aaatacggcg
     1201 ccgaaccgct gttaaacgca ttgtgcgaca aatccgcgac gccgcaagag acggatcagc
     1261 gctcggtttc cccgtcctta aggagcgccg ccgccatcgg ccgggtcacg gcgcgcccgc
     1321 gcgccaaggc ctcgcggtcg agcgcggcga cgatcgcgcg cgccgcggcg agcgagcgct
     1381 cgatccggag cgcgatatag tcgatgacag ccggctcgac gcgcaattga cggtcgtcga
     1441 acaatttcac cagcaccgcc cgcgccaagg cttcatccgg ctcgcgaagc gcggcgagcg
     1501 gcgcaaggcg caggcgcgac aggagatcgg ccttttcgag accccaaagg tcgggcgccg
     1561 cattcgccgt cagcaacagc gaagcgccgc gctcgcgcgc cagattgacg agatggaaga
     1621 gcgccgcctc ggcggcgccg agccgatccg cgtcgtcgat cagcagcgcc ttggcgacgg
     1681 caagctgcgg caggtcagcc tcgccgagcg cctcgccctt cagcttgctc gcgccggcgc
     1741 gctcggccca gattgcgccg agatggctct tgcccgagcc ggccgggccg accagcagca
     1801 gcatgcggcc cggccagtcc ggccaagcgt cgatcgcgcg cagagcttcg acattcgacg
     1861 gagcggcgag aaagtcgtcg cgcgaatagc gcggctcgtg cggcagatcg agaatcagtt
     1921 ggcgcgccag accagtcatc gcgccgctcc cggacaagcc gccgcgccgg ccgcgccccg
     1981 cttcccttcg cctctcatcg ccgccttggc tgcagcaaat cccatcgatt gccccagcga
     2041 tcctcgaaaa ccgccaccca cccataagac tcgtcgcgag gcgcctcaac aaagcgcacg
     2101 cccttggcga gaaggcgacg atgatcgcgc cagaaatcgt cggtatgaag gaaaaggaac
     2161 acccggccgc cggtttgatc gccgatccgc gcggcctgcc ggtcgtcggc cgccttggcg
     2221 agaagcagcc gcgcgccgcc gcccggcggc gccaccagga cccagcgctt gcccggcgcc
     2281 agcggcttat cttcgaccaa gccgaatccg agcgtctcgg tgaaataggc gatcgcctcg
     2341 tcgtaatcgg ggacgacgaa ggtcagcgcg ccgatcgtct ggctcacgtc gccgctccct
     2401 tgttcatgtg ttcgagccat tgcgccacat agaccgcgcc ggaggcgatt gtggaggcgc
     2461 cgacgagcca aaccgcgaat tcctcgcccg gcaggggcgc gaagccgaag gctttggccg
     2521 ccaggaccaa agcggcgacg gctatctgcg cgaccgtgtt gaatttcgac acccaaaccg
     2581 gccggatcgc cacgggattg tcgagcaacc aggagatcag caccgcgatc aggatcatca
     2641 ggtcgcgcga gacgacgatg atcgcgatcg ccgccggcaa ttcgccgacc acggcgagcg
     2701 tgacatagat cgagacgagc agcgccttgt cggccagcgg atcgagatag gcgccgagct
     2761 cggtgcgcag gtcgaagcgc ttggcgatga acccgtcgac cccgtccgac aggccggcga
     2821 tcacaaagat cgcgaaggcg ggcaaaaagc gctgcgagac gatcatgaga accgccagcg
     2881 gcgccagcag cagccgcgcc agcgtgatca aattgggcag gctggcgaaa aacttgtcga
     2941 attgcatcga gcggcgcgtc ccctcgtttt tcattccgcc ggccggctga agggccagct
     3001 tagctcaggc gcatccgcgc cgccgtccat acaaggcaag gcttgcctcg cccgccgaac
     3061 cgtcgcacat tccgggccga aaacggcagt ctggacgcgc tggacctata gtttggcgtc
     3121 cctgggggag ctttaaaaac gccgcatgac cgacgaagcg ccggatccgg cgatgcatta
     3181 cggcaatctg atcagcgcct acacggaagc gattcgcctc agcgatttta aagcgaatct
     3241 cacggttctg tttgtcgcga tcatgatggg gccggtcgtc gcctcgaaag acaaattccc
     3301 gcattttctg tcgctgcaaa tagcgctggc gcccttcctt gtcgcgttct tttgcctgct
     3361 gatgtgcgtc ttcccgcgct tcccgcggcg cggccggcgc aatttcctgg tctcgcgcaa
     3421 tccgcagcgt ggcgattttc tctattccga gcaccgagag gaagatgtcg ggcagttgaa
     3481 gctccggtgc gccatcctct cgggaatcct tttctggaag acgctgttcc tgaagatcag
     3541 ctttttcatc tgtttggcga cgctgatcgc gacctttctc ctgctgacct attcgctgct
     3601 ttgatgggac gcggctgaca tggcgagcga cgcaaagacc tattatcgca accagatcgc
     3661 agcctacacg gacacgatcc ggatcagcga cttcaaggcg aacgtcgcga tcatttatgg
     3721 cgcgttcacg atcggccctg tcatggggtt cgccgacaag tttccaccgt tcctgccgct
     3781 gaacgccgtg ctgctcccgt tcgtcgtcgt cttcttctgc ctgctcgtct gcctctttcc
     3841 gcgctatccg cgcgccggac gcgagacgtt cctcatcaag gcgaacgcca agccggagga
     3901 tttcgtcgtc cccgccaacc agcaggtcgt cctcgaaggc cagcaaacgc tctgcgtgat
     3961 cctctgcaac atcctctact ggaagacgat catgttgcag atcagcttcg cgatctacat
     4021 cctcggcacg ctcctggcgg cggccctgct ggcctatttc aagctgtaac ggccgcggcc
     4081 tgaagtcggg ccgccgcggg atttcctaaa catctgaaat tatgcgaggt tgatcgagcg
     4141 gaatgtggcg aatctcacat ggattcgccg ccggccgagg aataattggc gtcgccgtct
     4201 gttggaggct cctccttgtc gcgggtcgct tttggcgtcg cttagcccca ccgtcccgct
     4261 cgggagtttc tgcgccctgc ttctggcgtt ggcggtcaag catttcgtcg ccgattttct
     4321 ctttcagacc cattggatgg cgatgggcaa ggagcgctgc gaaggctggc tggcgcccct
     4381 ggcggcgcat gtcctctgcc acgccgtcct gaccctgtgc atcgccctgg tcgtcgcgcc
     4441 gcgcctttgg tggctggctc tcgtcgacct tgccatccat acctgcgtcg accgctgcaa
     4501 aagcattgtc ggccatcgca gcggctggaa catgaccaag ccgcaatatt ggtggcttct
     4561 cggcttcgac cagttcctgc accagatcac caatatcggc ctcgcggcgg ccttctacct
     4621 gctctgagca tcgcgcgcct gccgcttgcg tccggcccgg ccagcctata ggcgaagccg
     4681 acagcatgac cgacgcgccg cgcccatcgc tgacctatgc cgaagccggc gtcgatatcg
     4741 acgccggcgc cgcgatggtc gaggcgatca agccgttggt ccgcgccacc cggcggccgg
     4801 gcgccgacgc cgagatcggc ggctttggcg gcctgttcga tctcaaagcc gcgggcttcg
     4861 ccgatcccgt cctggtcgcc gccaatgacg gggtgggcac caaggtcaag atcgccatcg
     4921 actgcgccat tcacgacacg atcggaatcg acctcgtcgc catgtgcgtc aacgacctcg
     4981 tcgtgcaggg cgccgagccg ttgttcttcc tcgactatta cgcgaccggc aggctcgatc
     5041 cggcagtcgg cgcggcggtg gtcaagggga tcgccgccgg ctgtcgcgaa gccggctgcg
     5101 ctctgatcgg cggcgagacc gccgaaatgc cgggtcttta cgccgggcgc gactacgacc
     5161 ttgccggctt cgccgtcggc gccgccgagc gcgggcgcct attgccgaaa ggcggactga
     5221 aaccagggga catcgcgctc ggcctcgcct cctccggcgt tcactccaac ggcttctcgc
     5281 tggtccgccg gatcgtcgag cgccgcgccc tgcccttcga cgcgccggcg cccttcgcgc
     5341 caggcctatc gctcggccaa gcgctgctcg cgccgacccg catctatgtg aagccgctgc
     5401 ttcaggcgct cgccgccagc cccgccattc tggcgctggc gcatattacc ggcggcggct
     5461 ttcccgacaa tcttccccgc atcttgcctg acgggctcgg cgtcgcagtc gatcttggcg
     5521 ctttcgcgcc gccgccggtt ttcggctggc tcgccgaggc gggcgggctc gcgcaatcgg
     5581 aaatgctgcg cacgttcaat tgcgggatcg gcatggtcgc cttcgctgcg gcggaaggcg
     5641 cggcggaagc ggcggcggcc ttggccgccg ccggcctcgc gccagtccaa atcggcgaag
     5701 tcgtcgccgc cgcgagcggg ccgcgcgtgc gtttttccgg caggctcgtc ctttgatctt
     5761 gcgcaagcgc accgcgatcc tgatttccgg ccgcggctcc aacatggccg cgctgatcgc
     5821 ggcggcgcgc gcgccggatt ttccggccga gatcgttctc gttctctcca acaagccgga
     5881 cgccgagggc ctggcgcgcg ccgagagcgc cggaatcgcc gtcgccgcgg tcgatcacaa
     5941 gatttacgcc ggccgcgacg agttcgaagc ctcgatgcag gctctgctcg acatccaccg
     6001 cgtcgagctg atctgccttg ccggcttcat gcgggtgttg agcccgcctt tcgtgacgcg
     6061 ctggcgcggc cggatgctca acatccatcc ctcgctcctg cccgccttcc gcggcctcga
     6121 cacgcatcgg cgcgccttgg acgccggcgt gaagatccat ggctgcacgg tgcatttcgt
     6181 cgagccggag ctcgattccg gcccgatcgt cgcgcaaagc ttcgtttccg tctgcgacga
     6241 cgatacgccg gagacgctcg ccgcgcgggt gatcgagaaa gagcacgcgc tctacgccga
     6301 ggctttggcc ctggtcgcgg cgggacggat cacgctcgaa agcggcgtcg cgcccaagag
     6361 ttgatcacag cttcgcgtct gccgccgtca tttcgacaca gccgattgct attcagaaag
     6421 attggcgaag cggcggacgt cgcgtagtcg ccgccgccca acgaacccgc gctttgtgag
     6481 cgcgctgcgt tctttagaac attttctgtg ggaggcggat atgcggcaga tggtctctct
     6541 ctccctttgc gcgagcatcg tcatcggaag ctgcggcgcg gccgcggccg ccgatgtttc
     6601 gacctggcgc tggaaaacct cgcaagcgtt gcgcgagcag caagaccgcg aagcggacgc
     6661 cgcgaaagtc tatcgcgagg cgagcgcgtt cgaagtcacc ccgggcatgc agcagcgcgg
     6721 cgacccggcg gccggcgccg acccgctcta ttccggcgat ttgaaagcgg cggtcgaggc
     6781 gcagcgctcc ggccagtaag cccgcattca atccgtgcgg agcacgaagc cggcgcggcg
     6841 cttgttgacg atgagggggc ggtccgcgcc cacgtcgagc ttgcgcctca gattgctcaa
     6901 atgtacgtcg acggtgttgg tcaagcgcac cgagcgataa cgccagacgt gttcgagcag
     6961 agcctcgcgg gtgacgacct cgccgggatg gcgcatgaaa tattcgagga ttttgaattc
     7021 gcgcgggagc agctcgatcg gccggccgtc gcgccgaacc gactgctcga tcaaatccat
     7081 ttcaaggggg ccggccttga gccgcgtcgc gcgattgtcg ccgagccgcc gcgcgatggc
     7141 gtcgatccgc gccatcaact cgcccagagc gaaaggtttc acaaggtaat cgtccgcccc
     7201 ggcgcgcaag ccgcggatgc gctcctcggc ggattccgag gcgctgatga tcacgatggg
     7261 cgcgacgacg ccccgcttgc gcatcgtctc caccagttcc agcgaatctt cgccaagcag
     7321 gaggcgatcg aggacgatga cagccgcgtc ctcggcaaac gcagcgcgca gcgcgtcgcg
     7381 tttgtcctgc aaaatttgtg gcgcaaatcc gtggcgtttc aactcggcgc caatttcgct
     7441 ggcga
//
LOCUS       DATGJW010000515         4820 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_720808, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000515 DATGJW010000000
VERSION     DATGJW010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4820)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4820)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4820
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_720808"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(336..1280)
                     /locus_tag="VKU03_15665"
     CDS             complement(336..1280)
                     /locus_tag="VKU03_15665"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019173318.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HLJ72753.1"
                     /translation="MSDITRAGAASPIPGSIDETLALLRKADYIGERSLATVLFLSLR
                     LGRPLFLEGEAGVGKTEIAKVLAATLGRRLIRLQCYEGLDVAAAVYEWSYASQMMAIR
                     LAEAAGEHDKERIEHDVFSERYLIKRPLLQALEPAPEGAPVLLIDEIDRADEAFEAFL
                     LEVLSDFQVTVPELGVIKAEHPPIVVITSNRTREIHDALKRRCLYHWLGYPDAAREIE
                     IVKAKAPGAPKRLTEELVAFVQSLRRQDLFKAPGVAETLDWASALTELDAVALDPAQV
                     SDTLGVLLKYQDDIAKIEGAKAAELVESVRSELRAGAR"
     gene            complement(1523..2320)
                     /locus_tag="VKU03_15670"
     CDS             complement(1523..2320)
                     /locus_tag="VKU03_15670"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012111885.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein subunit M"
                     /protein_id="HLJ72754.1"
                     /translation="MYQFTYHRPDSLSAAVAQLAADSDAKALAGGQTLLPTMKQRLAA
                     PSAIVDLGKIAELAGISREGDAIVIGAMTRHADVATSAAVHQAIPALAALAEGIGDPQ
                     VRNKGTMGGSLANNDPAADYPAAALALGATIVTNKREIAADAFFKSLFETALEPGEII
                     VKVRFPIPQKAAYVKFPNPASRYALVGVFVARNAGGVRVAVTGAGANGVFRANAIEAA
                     LASNFSASALAGLKIPADGLNSDIHADAAYRAHLIGVLAKRAVAAAG"
     gene            complement(2406..4772)
                     /locus_tag="VKU03_15675"
     CDS             complement(2406..4772)
                     /locus_tag="VKU03_15675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009763553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HLJ72755.1"
                     /translation="MSATGIGASVKRKEDIRFITGKGHYTDDINRPGQAYAYFIRSPH
                     AHATINKIDASAATKAPGVVAVFTGDDLAKDKVGGLICGWMIHSKDGSPMKAGAHPAL
                     AQGKVRYVGDHVAVVIADTYAQAKDAAEKVVIDYGVLPAAIDLAATTKAGAPQIHDVA
                     PNNTVYNWHLGDKAATDAAFAAAKHVTKLDLVNNRLIPNAMEPRAAVGEYDPGTDTFT
                     LYTTSQNPHVTRLVLAAFIGIAPEHKLRVIAPDVGGGFGSKIFIYAEETVSVWAAKKV
                     GRPVKWTAERTESFLSDAHGRDHLTHAELALDASGKITGLRVHTVANLGAYLSTFSSA
                     VPTYLYGPLLSGQYAIPAIYCEVDAVYTNTSPVDAYRGAGRPEATFVVERLVEIAARE
                     TGRDPAEFRRANFVTAFPHQTPVAFNYDAGNYAAALDKALALADYKGIGARKAASAAK
                     GKLRGIGFSAYIEACGLAPSAMVGSLGAGVGQWESAEVRVNPTGSVEVLTGSHAHGQG
                     HETTFAQLVSDRLGIPIENVSVVHGDTDKVQFGMGTYGSRSGAVGMAAISKALDKVEA
                     KAKKVATHVLEAAEGDIEFKDGKFTVKGTDRSIDFGSIALQAYVAHKFNGQELEPGLK
                     ETAFWDPTNFTFPAGVHICEVEIDPETGVTKIDRWTAVDDFGVLINPMIVEGQVHGGV
                     AQGVGQALLEGAVYDADGQLVTASYMDYCMPRADDLPSLTVATTETRCPSNPLGIKGC
                     GEAGAIASPPAVINAITDALGHEDVAMPATPQVVWRAAQKANLKRAAE"
BASE COUNT          783 a         1552 c         1631 g          854 t
ORIGIN      
        1 ggtcgccgac gcccgcggcc tgcaccgcgg ccagcgcgtc aagcaccgcg cccggcccga
       61 ccggcaggcc ggcctcgcgc aacgcgcggg cgaaatagag gatgttggtc gcgagttggc
      121 cggagccgga cgcggcggga gaggatggcg ggtctaagtc catcgataaa actgcaaagt
      181 acactccgcg tcgcggctca ccctcatccg gcgcttcgcg ccaccttctc ccgcaagcgg
      241 gagaagggat ttgagccgcg gcgcgccccc tctcccgctt gcgggagagg gttggggtga
      301 gggtacgccg cacgtttcga acaaatttat cctgctcacc gcgcgcccgc gcgcaattcg
      361 ctgcgcacgc tctcgaccag ttcggccgcc ttggcgccct cgatcttggc gatgtcgtct
      421 tgatacttca gcaagacgcc gagcgtgtcg gagacttgcg cgggatcgag cgcgacggcg
      481 tcgagctcgg tcagggcgct cgcccaatcg agcgtctcgg cgacgccagg cgccttgaag
      541 aggtcctgcc ggcgcaagct ctgaacgaag gcgacgagct cctcggtcag acgcttcggc
      601 gcgccgggcg ccttggcctt gacgatttcg atctcgcgcg cggcatccgg gtagccgagc
      661 caatgataga ggcagcggcg cttcagcgcg tcgtggattt cgcgggtgcg gttggaggtg
      721 atgacgacga tcggcggatg ctcggccttg atcacgccca gttccggcac cgtgacttgg
      781 aagtcagaga gcacttccag gaggaaggct tcgaaggctt cgtcggcgcg gtcgatctcg
      841 tcaatcagca ggaccggcgc gccttccggc gccggctcca aggcctggag caacggacgc
      901 ttgatcagat agcgctcgga gaagacgtcg tgctcaatcc gctccttgtc gtgctcgccg
      961 gccgcctcgg cgagacggat cgccatcatc tgagaggcgt agctccattc atagacggcg
     1021 gcggcgacat cgagcccctc gtagcattgc aggcggatca gccggcgtcc gagcgtcgcg
     1081 gcgaggacct tggcgatctc cgttttgccg acgccggcct cgccttcgag gaagagcggc
     1141 cggccgagcc ggaggctgag aaacagcaca gtcgccagcg agcgctcgcc gatataatcg
     1201 gccttgcgca aaagcgcgag cgtctcatcg atcgagccgg gtatgggcga agccgcgccg
     1261 gctcgagtga tgtctgacat gccttgaccg ctccaaaagt ccgtaccgcg tttccagaac
     1321 gcctgtcccg ggcctccgtt tcatacaagg cggcggaagg caaggcgcgg gccctctcct
     1381 cccccgtcgc agacggggga ggggaccatg cgaagcatgg tggagggggc cgccggcgca
     1441 tcaggataaa tcccaaccgc cgaggccccc ctccaccgcg cttacgcgcg gtccccctcc
     1501 cccgcttcgc gggggaggat attcaccccg ctgcggcgac ggcgcgtttg gcgaggacgc
     1561 cgatcagatg ggcgcgatag gcggcgtcgg cgtggatgtc gctgttgagc ccgtccgcgg
     1621 gaattttgag gccggccaaa gccgaagccg agaaattcga cgccagggcg gcttcgatgg
     1681 cgttggcgcg gaagacgccg ttggcgccgg cgccggtcac cgcgacgcgc acgccgcctg
     1741 cattcctggc gacgaacacg ccgaccagcg catagcgcga ggccggattg gggaacttca
     1801 cgtaagccgc cttctgcggg atcgggaacc ggaccttgac gatgatctcg cccggctcga
     1861 gcgcggtctc gaacaagctt ttgaagaacg cgtccgccgc gatttcgcgc ttgttggtga
     1921 cgatcgtcgc gccgagcgcc aaagccgccg ccgggtagtc ggcggccgga tcgttgttgg
     1981 cgagcgagcc gcccatcgtg cccttgttgc gcacttgcgg atcgccaatc ccttcggcga
     2041 gcgcggccaa agccgggatc gcctgatgca cggcggcgct ggtcgcaacg tcggcgtgac
     2101 gggtcatcgc gccgatgacg atcgcgtcgc cctcgcggct gatcccggcg agttcggcga
     2161 tcttgccaag atcgacgatc gcgctcggcg cggcgaggcg ctgcttcatc gtgggcagca
     2221 aggtttggcc gccggccagc gccttggcgt cgctgtcggc cgccaattgc gcgacggcgg
     2281 ccgagaggct gtcgggccga tggtaggtga attgatacat gggatgctcc ctagaattgg
     2341 tgagtggcga gtggtgaata gcgaatagcc attcgccact ccctattcgc tcttcgctct
     2401 ttgtctcact cggcggcccg tttcagattc gccttctgcg ccgcgcgcca gacgacttgc
     2461 ggggtcgccg gcatggcgac atcctcatgg ccgagcgcgt cggtgatcgc attgatcacc
     2521 gccggcggcg aagcgatcgc gccggcctcg ccgcagcctt tgatgccgag cgggttggac
     2581 gggcagcgcg tctccgtggt ggcgacggtg agcgacggca agtcgtcggc gcgcggcatg
     2641 caataatcca tgtagctcgc cgtcaccaat tggccgtcgg catcgtagac cgcgccttcc
     2701 aacaaggcct ggccgacgcc ctgggcgacg ccgccatgga cctgaccttc gacgatcatc
     2761 ggattgatca gcacgccgaa atcgtcgacc gccgtccagc ggtcgatctt ggtgacgccc
     2821 gtctccggat cgatctcgac ctcgcagata tggacgccgg ccgggaaggt gaagttggtc
     2881 ggatcccaga acgcggtctc cttgaggccg ggctcgagct cctggccgtt gaatttgtgg
     2941 gcgacatagg cctgcaaggc gatcgagccg aaatcgatcg agcgatccgt acccttgacc
     3001 gtgaacttgc cgtctttgaa ctcgatatcg ccttcggcgg cttcgagcac atgcgtggcg
     3061 accttcttcg ccttggcctc gaccttatcg agcgccttgg agatcgccgc catgccaacc
     3121 gcgccggagc gcgagccata ggtccccatg ccgaattgca ccttgtcagt gtcgccatgg
     3181 acgaccgaga cgttctcgat cggtatgccg agccggtcgg agaccaattg cgcaaacgtc
     3241 gtctcatggc cctggccatg ggcgtgcgag ccggtcagga cctcgacgct gcccgtcgga
     3301 ttgacgcgca cctccgccga ttcccactgg ccgacgccgg cgccgagcga accgaccatc
     3361 gccgagggcg cgaggccgca ggcttcgata taggcggaga agccgatgcc gcgcagcttg
     3421 cctttggcgg cggacgccgc cttccgcgcg ccgattcctt tatagtcggc gagcgcgagc
     3481 gccttatcga gcgccgcggc gtaattgccg gcgtcgtagt tgaaggcgac cggcgtctga
     3541 tgcgggaacg cggtgacaaa attggcgcgc ctgaactcgg ccgggtcgcg cccggtctca
     3601 cgcgcggcga tttcgacgag ccgctcgacg acgaacgtcg cctccggccg cccggcgccg
     3661 cgataggcgt cgaccggcga ggtgttggtg tagaccgcgt cgacctcgca atagatcgcc
     3721 gggatcgcgt attggcccga caacagcggc ccatagagat aggtcggcac cgccgaggag
     3781 aaggttgaaa gataggcgcc gagattggcg accgtatgga cgcgcaggcc ggtgatcttg
     3841 ccgctggcgt cgagcgccaa ttccgcatgc gtcagatgat cgcggccatg cgcgtcggag
     3901 aggaaggatt cggttcgctc cgcggtccat ttcaccggcc ggccgacctt cttggccgcc
     3961 cagacgctga ccgtctcttc cgcatagatg aagattttcg agccgaagcc gccgccgaca
     4021 tcgggcgcga tgacgcgcag cttgtgctcc ggcgcgatgc cgatgaaggc ggcgaggacc
     4081 agccgcgtca catgcgggtt ctggctggtc gtatagagcg tgaacgtatc ggttcccgga
     4141 tcgtattcgc cgaccgccgc gcgcggctcc atcgcgttcg gaatcagccg gttgttgacg
     4201 aggtcgagct tcgtcacatg cttggcggcg gcgaaggcgg cgtcggtcgc cgccttgtcg
     4261 ccgaggtgcc aattatagac cgtgttgttc ggcgccacgt catggatctg cggcgcgccg
     4321 gctttggttg tcgcggcaag gtcgatcgcc gcgggcagga ctccataatc gatcacgacc
     4381 ttttcggcgg cgtccttggc ctgggcatag gtatcggcga tgacgacggc gacgtgatcg
     4441 ccgacatagc gaaccttgcc ttgcgccaaa gccggatggg cgcccgcctt catcggcgag
     4501 ccgtccttgg aatggatcat ccagccgcaa atcaggccgc cgaccttgtc cttggcgaga
     4561 tcgtcgccgg tgaaaaccgc cacgacgccg ggcgctttcg tcgcggccga ggcgtcgatt
     4621 ttattgatcg tggcgtgggc gtggggagaa cgaatgaaat aagcgtaggc ttgccccggc
     4681 cggttgatgt cgtcggtgta atggcctttg ccggtgatga agcggatatc ttccttgcgc
     4741 ttgactgagg cgccaattcc ggtcgcgctc atgattttcc tcctgatgcc gccgatcttg
     4801 ccccaacggg tgatcggcaa
//
LOCUS       DATGJW010000516         2347 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_308891, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000516 DATGJW010000000
VERSION     DATGJW010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2347)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2347)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2347
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_308891"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            280..753
                     /locus_tag="VKU03_15680"
     CDS             280..753
                     /locus_tag="VKU03_15680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018096478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HLJ72756.1"
                     /translation="MAKNTVCLWYDKDAEAAARFYAETFPDSAVGAVHHAPSNYPSGK
                     EGDVLMVEFTVAGIPCIGLNGGPVFTHNEAFSFQIATDDQQETDRYWNAIVGNGGQES
                     ACGWCKDKWGVSWQITPRVLTEALAAGGAEAKRAFDAMMDMRKIDVAAIKAARRG"
     gene            complement(806..1345)
                     /locus_tag="VKU03_15685"
     CDS             complement(806..1345)
                     /locus_tag="VKU03_15685"
                     /inference="COORDINATES: protein motif:HMM:NF013625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan-binding domain-containing protein"
                     /protein_id="HLJ72757.1"
                     /translation="MAQAVIRRLAARPSRTIAGAAFAAIMTGIVINALFLQKTHRFAV
                     IAPPASTAPVRPPPAAAPPTQAAAPSEAAPAPPARPADLGALIEAAAAPHAGDPIRDL
                     LRAEPAAKESGEAKRLTLAAQNALIKLGFAIKADGVDGASTRQAIQQFERAHGFIPLG
                     EITPKLVRQLTAAASEAGR"
     gene            complement(1419..>2347)
                     /locus_tag="VKU03_15690"
     CDS             complement(1419..>2347)
                     /locus_tag="VKU03_15690"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016918196.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HLJ72758.1"
                     /translation="MRARRFAGAAGPCDVDGAAVISVARDISAAKAYEAALEAARAEA
                     EVANSWKDRLLANVSHELRTPLNAIIGFSEILASPDLKPRDPARQLEYAGIIHNSAEH
                     LLSVVNLVLDASKIEAGQFELLPEPFDIAALISACCDMVRLKADKGRVALVRAPVDCP
                     SELIADKRACRQIVLNLLSNAVKFTLPEGRVTIGAQSDGTMLTLYVEDTGIGIAQGHL
                     SRLGDRFFQVRSDADRLFEGTGLGLSVVRGLVGLHGGSVALESAAGKGTRVIVTLPLD
                     CRPTARSDAPPARLEANASSTFHPMMKDKKIA"
BASE COUNT          414 a          760 c          821 g          352 t
ORIGIN      
        1 ttcgacggcg cagcggcgga tataggcggc aacccagatg tcggcgcgca ggcgcatggg
       61 agagatgtag agcgcgcgaa agcaaagttg aagccggctt ttggcgcgac gagccgtcga
      121 gcgaagccta tatggcggga ggcgagaagc gcaggcggcc attctttgga acgaagccat
      181 gcgtggccag aaaagaaccg gctgccctct ggagaaaggc ggtctgcctc ccatctcagg
      241 caaacgcgcg aattgacctc aaagcggaaa ggataggcaa tggccaagaa cacagtttgc
      301 ctttggtatg acaaggacgc cgaggctgcg gcccgcttct acgccgagac ctttcccgac
      361 agcgcggtag gcgccgtcca ccatgcgccc agcaactacc cgtccggcaa ggaaggcgat
      421 gtgctgatgg tcgaattcac ggtcgccggg ataccctgca tcggcctcaa cggcggcccc
      481 gtcttcacgc acaacgaagc cttctcgttc cagatcgcca ccgacgatca gcaggagacc
      541 gaccggtact ggaacgcgat cgtcggcaat ggcggccagg agagcgcgtg cggctggtgc
      601 aaagacaaat ggggcgtctc gtggcaaatc acgccgcgcg tgctgacgga ggcgctggcg
      661 gccggcggcg ctgaggcgaa gcgcgccttc gacgccatga tggacatgag gaaaatcgac
      721 gtcgccgcga tcaaggcggc gcggcgcggc tgacgatcga gcctggcgcg gagatccccc
      781 ggagccaagc gtcagatttc agccgctaac gccctgcctc gctggcggcg gcggtcagct
      841 gcctgaccag cttcggcgtg atctcgccga gcggaatgaa gccgtgcgcg cgctcgaatt
      901 gctgaatggc ctgccgcgtc gaggcgccgt cgacgccatc ggccttgatc gcgaaaccga
      961 gcttgatcag cgcgttttgc gcggcgaggg tcaagcgctt cgcctcgccg ctctccttgg
     1021 cggcgggctc ggcgcggagc aagtcgcgga tgggatcgcc ggcatgcggc gcagcggcgg
     1081 cttcgatcag cgcgccgaga tcggcgggtc gcgccggcgg cgccggggcg gcctctgaag
     1141 gcgccgcggc ttgcgtcggc ggcgcggcgg ccggcggcgg cctcaccggc gcggtcgagg
     1201 ccggcggcgc aatgacggcg aagcgatgcg tcttctgcaa gaagagcgcg ttgatgacga
     1261 tgcccgtcat aatcgccgcg aaagcggcgc cggcgatcgt gcgcgaaggc cgcgccgcca
     1321 ggcgccggat gaccgcttgc gccaacgacc tgcgctcgct ccgctggccg cgcgccgagc
     1381 gtgaaagaac ggaatcatga tgcgagcggg cgagcgcctc aggcaatttt cttgtctttc
     1441 atcatcgggt gaaaggtcga cgaggcgttc gcctccagcc gcgccggcgg cgcgtcgctg
     1501 cgcgccgtcg gccggcaatc gagcggcagc gtgacgatca cccgggtgcc cttgccggcg
     1561 gcgctttcca gcgccaccga gccgccatgc aggccgacca ggccgcgcac caccgaaagg
     1621 ccgaggccgg tgccctcgaa cagccggtcg gcgtcggagc gcacttggaa aaaccggtcg
     1681 cccagccgcg acagatggcc ctgcgcaatg ccgatgccgg tgtcctcgac atagagcgtc
     1741 agcatcgtcc cgtcggattg cgcgccgatc gtcacgcggc cttccggcag cgtgaatttg
     1801 acggcgttgg acaaaagatt gagtacgatt tgccggcagg cgcgcttgtc ggcgatcagc
     1861 tcgctcgggc aatcgaccgg cgcgcgcacc aaggcgacgc ggcccttatc cgctttgagc
     1921 cgcaccatgt cgcagcacgc gctgatcaag gcggcgatgt cgaaaggctc gggcagaagc
     1981 tcgaattgcc cggcttcgat tttcgacgca tcgagcacga gattgaccac cgagagcagg
     2041 tgctcggcgg aattgtgaat gatcccggca tattcgagct gtcgcgccgg atcgcgcggc
     2101 ttgaggtcgg ggctcgccag aatctccgaa aaaccgatga tcgcgttgag cggcgtgcgc
     2161 aattcgtggc tgacattggc gagaaggcgg tccttccagg aattggcgac ttccgcctcg
     2221 gcgcgcgccg cttcgagcgc cgcctcatag gctttggcgg cgctgatgtc gcgcgccacc
     2281 gagatcaccg ccgccccgtc gacgtcgcac gggccggccg cgccggcgaa gcgccgcgcc
     2341 cgcattt
//
LOCUS       DATGJW010000517         8048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1236281, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000517 DATGJW010000000
VERSION     DATGJW010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 8048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8048
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1236281"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..857)
                     /locus_tag="VKU03_15695"
     CDS             complement(<1..857)
                     /locus_tag="VKU03_15695"
                     /inference="COORDINATES: protein motif:HMM:NF019128.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell wall hydrolase"
                     /protein_id="HLJ72759.1"
                     /translation="MESAQAPIRQALLAANFHLPGGVGAFGAPSLIQASLTLGSPEDL
                     AEVDDEIDPRADLKQPRRAFPVVDRSLKSDPFMALRPGFNAKLNRRLRRGDAAPVDPG
                     ALSSFDPDAGEAVLSYVDMPPVDQAAPWSDSNADDRPLTVSDGATPAVPLDVALNSAT
                     PQPSDDKPIVVASAAPEATSGDSIEVAKSLPNGQPDYASLIDPKDSARQQRCLAEAIY
                     FEARSEPEEGQAAVAQVVLNRVRSGLYPSNVCAVVYQDRNRPFACQFSFACEGKSLRI
                     EEPGAWAVAT"
     gene            complement(1180..1737)
                     /locus_tag="VKU03_15700"
     CDS             complement(1180..1737)
                     /locus_tag="VKU03_15700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018181988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupin domain-containing protein"
                     /protein_id="HLJ72760.1"
                     /translation="MAKQDPDVGQRLRQARRRLGLSQRELARRAGVSNGTISMIEANQ
                     VSPSVSALKQVLSAIDIGIADFFAPAETESEQIVFRADQLTEIAGGPVSYRQVGANLR
                     GRSLQMLHERYRPGAQSGRPMLSHQGEEAGIVIKGRLGLEVEGRRYELGPGDAYFFDS
                     RKPHAFRNLGDGELVVVSACTPPSF"
     gene            2047..3384
                     /locus_tag="VKU03_15705"
     CDS             2047..3384
                     /locus_tag="VKU03_15705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177029.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HLJ72761.1"
                     /translation="MSALASRIDSPNDLEAFWLPFTPNRAFKKRPRLIARSKDMHYYT
                     PEGRPILDATEALWCCNAGHNRPRIVEAIQRQAAELDFAPAFQFAHPQAFALSARLAA
                     LAPADLDHVFFANSGSEAADTALKIAIAYHNVRGQGARQRLIGRERAYHGVGFGGISV
                     GGMVNNRKFFGSLLAGVDHLPATYNRERQAFTKGEPEWGGHLADELERIVALHDASTI
                     AAVIVEPMAGSTGVLPAPKGYLQKLRAICDKYGILLIFDEVITGFGRLGAAFAAERYG
                     VIPDIITFAKGVTSGTVPMGGVIVRKHVHDAFMKGPEQAIELFHGYTYSAHPLACAAS
                     LATLDTYRDEKLFERAKALEPKWCDAIMSLKGLPNVLDIRCVGLTGAIDLAPSGGGAG
                     ARGYAAVEHSFHEEGIMIRISGDTIELTPPLIVSEDQIGEIVDKVGKTIKAVA"
     gene            3483..3899
                     /locus_tag="VKU03_15710"
     CDS             3483..3899
                     /locus_tag="VKU03_15710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016919115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF559 domain-containing protein"
                     /protein_id="HLJ72762.1"
                     /translation="MRGIRIPETKRARALRRDASDAERKLWRVLRARSLDGRKFVRQE
                     RIGPYYADFACREAKLVVEIDGATHSTEEERARDRGRTALMTRHGYRVVRFTNIEVYE
                     QIDRVCEAILVALAERQSPVDPPLSVPLPASRGEEA"
     gene            3952..4902
                     /locus_tag="VKU03_15715"
     CDS             3952..4902
                     /locus_tag="VKU03_15715"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006615469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72763.1"
                     /translation="MLLYLLQRLLLLLATLAFASLVVFGVLEILPGNAAQVMLGASAT
                     PEAVAALAHKLGLDQPFAVRYVAWLSGIAHGDLGLSYAYNTSIGALIADRLSVSGPLA
                     LIAMILTSVIALAAGLFAAQRRGKNGDFAVMAASQIGLAVPNFWFAILLVLIFAVKLR
                     IAHSGGFPGWSAGFGQALGALILPAISLGLVQAAILTRVTRSALLDVLNEDFIRTARA
                     KGLSRRAALWRHALPNALTPILTIMGLQFANLIAGAIIVENVFVLPGIGQLIFQAIAN
                     RDILVVEDCVLLLAAIVIVVNFVVDVACAAIDPRLRRRAL"
     gene            4899..5741
                     /locus_tag="VKU03_15720"
     CDS             4899..5741
                     /locus_tag="VKU03_15720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669018.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72764.1"
                     /translation="MSKDGFLSRAKRNPSFLIGAALVLLVACAAALSLIWTPYPPAAI
                     DVPHKLLTPSAQHWLGTDSLGRDVASELLAGARISILVGVIAVGIGLVFGVALGLFAA
                     SAKGLIEDIVMRFSDFAFAFPALLMAIMLTATFGAGVVNAIIAIGIFNVPLFARVTRA
                     SANTVLSREYILAARAAGKGPIAIAVEHVLPNIAPILIVQATIQFAIAVLAEAALSYL
                     GLGAQPPQTSWGRMLSEAQNLMYQAPRLAIFPGLAIVVAVLGLNLLGDGLRDLLDPRL
                     RARR"
     gene            5964..6938
                     /locus_tag="VKU03_15725"
     CDS             5964..6938
                     /locus_tag="VKU03_15725"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007593199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HLJ72765.1"
                     /translation="MLLEIAGLNIDLPTPDGPRRIVRDVSFSLARGETLGVVGESGSG
                     KTMTALALMGLSPEGAALSGAVRFDGEDLARIDEAAWRRLRGDRIAMIFQEPMTALNP
                     LQTIGRQIAEPLILHRGLRRGAAMQEAVKLLARVGLPDPARRARAYPHQLSGGQRQRA
                     MIAMALSCGPKLVIADEPTTALDVTVQAQILSLLAELREQTDMAMILVSHDLAVIAQN
                     CQRVVVMYGGMVMESGATADVLERPAHPYTRALLQARPRLGGTRGQRLRVIPGSAPTA
                     GETPKGCPFAGRCPLTIAACSAAPPPPFPVGSGRIARCIRLEEAEKVA"
     gene            6935..7897
                     /locus_tag="VKU03_15730"
     CDS             6935..7897
                     /locus_tag="VKU03_15730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015669016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HLJ72766.1"
                     /translation="MSEPLLQLIDLAKTYRLASEKPFGPKKLLRAVDGVSLAIAAGRS
                     FGIVGESGSGKSTLARCAIALEKPSSGEARLMGQPLGKASRRELRAMRARMQMIFQDP
                     YGSLDPRQTVARIIAEPLAVLDGVSRAETAKRVAESLEAVGLGAADGRKYPHEFSGGQ
                     RQRIAIARALITRPKLVVADEPVSALDVSVQAQVLNLMMDLQQSAGVTYLLISHDLAV
                     VEHVCEEIAVIYRGRFVETGPTEKLLAAPAHPYTRALLDAAPKLKVERPPAAAPDAPT
                     APESGAGCAYAERCPFAQERCRAEAPALRPLADGRQAAYHFPLD"
BASE COUNT         1241 a         2803 c         2631 g         1373 t
ORIGIN      
        1 gtcgcgaccg cccacgcgcc gggctcctcg atccgcagcg acttgccctc gcaggcgaag
       61 gagaactggc aggcgaacgg ccggttgcgg tcctggtaga cgaccgcgca gacattggac
      121 ggatagagcc cgctgcgcac gcggttgagc acgacctgcg ccaccgcggc ctggccttcc
      181 tcaggctcgc tgcgcgcctc gaaatagatc gcctcggcga gacagcgctg ctggcgcgcc
      241 gaatctttcg gatcgatcaa cgaggcgtag tcgggctggc cgttcggcaa actcttcgcg
      301 acctcgatcg aatcgccgct cgtcgcttcc ggggcggcgg aggcgacgac gatcggcttg
      361 tcgtcgctcg gctgcggcgt cgccgaattg agcgccacgt cgagcggcac ggccggcgtc
      421 gcgccgtcgc tgacggtcag cggcctgtcg tcggcgttcg agtcggacca gggcgcggcc
      481 tgatcgaccg gcggcatgtc cacataagag agaaccgctt cgccggcgtc ggggtcgaag
      541 gacgacaacg cgcccggatc gaccggcgcg gcgtcgcccc gcctcaggcg gcggttgagc
      601 ttggcgttga agccggggcg gagcgccatg aacgggtcgc tcttcaagct gcggtccacg
      661 accggaaagg cccggcgcgg ctgcttgaga tcggcgcgcg ggtcgatttc gtcgtcgacc
      721 tcggcaaggt cctccgggct gcccagcgtc aggctcgcct ggatcaggct gggcgcgccg
      781 aaagcgccga cgccgcccgg cagatgaaaa ttggcggcga gcagggcctg gcggatcggc
      841 gcctgggcgc tttccaaggc gaggctggcg ccccggctcg agtcttgccc cgcttgcgcg
      901 gttatcgaca caacgaggcc gccggccagg caccatggcg cgatcgcatt gcaagcccag
      961 cctaagcgcg aacgccccat acgcgaccca gctcggttcc ttcaagcccg tgatcaggcg
     1021 cgcttccgcc gcaaaacgac gcgcgtcgcc cccgtccggc gcggaaggaa tccctcaaat
     1081 ccgaacgacg atctttatcc cagcttaacg ttgcgagccg gttaccgcgc cgcacgtggc
     1141 cgcgtcccgg aatggctcat ccatgaagcg caggtaaaat caaaagcttg gcggcgtgca
     1201 ggcggagacg acgacgagct cgccgtcgcc gagattgcgg aacgcgtgcg gcttgcggct
     1261 gtcgaagaaa taggcgtcgc ccggtccgag ctcgtagcgc cggccctcca cttcgaggcc
     1321 gagccgaccc ttgatgacga tgccggcctc ctcgccttga tgcgagagca tcggccggcc
     1381 ggattgcgcg ccgggccggt agcgctcgtg cagcatttgc agcgagcggc cgcgaaggtt
     1441 ggcgccgacc tggcggtacg agaccgggcc gccggcgatt tccgtcaatt ggtcggcgcg
     1501 gaagacgatc tgctcgcttt ccgtctcggc cggcgcgaag aaatcggcga tgccgatatc
     1561 gatcgccgag aggacctgct tcagcgccga gaccgacggg ctgacctggt tggcctcgat
     1621 catcgagatc gtcccgttgg agacgccggc gcgacgggcg agctcgcgct gcgacaggcc
     1681 gagccgccgg cgcgcctggc gcaacctttg gccgacatcc ggatcctgct tggccatgtt
     1741 catgcgttca gtatttagaa cgccgtgaac agcgctgcaa atggacaaac ccgccttctg
     1801 ctggcactgt ttagaaaatc gatcatcgcg cgggtgcgct gagaaggcaa aagccgcggc
     1861 tcaccctcat ccgtccgctt cgcggccgcc ttctcccgca agcgggagaa gggactgcag
     1921 ccgcggtcgg cgaccgcggc tacagcgccg ctgagagggg attggccgcc gtctgcgacc
     1981 gcggctacag cgcaacgcgc cggccgacga aggcggcgta atttgggttc gctttaggag
     2041 aaagacatgt ccgcacttgc ttcgcgcatc gactcgccga acgaccttga ggcgttctgg
     2101 ctgccgttca cgccgaaccg cgccttcaag aagcggccgc ggctgatcgc gcgctccaag
     2161 gacatgcatt attacacgcc ggaaggccgg ccgatcctcg acgcgaccga ggcgctctgg
     2221 tgctgcaacg ccggccataa ccggccgcgc atcgtcgagg cgatccagcg ccaggcggcc
     2281 gagctcgatt tcgcgccggc ctttcaattc gcccatccgc aggctttcgc gctgtcggcg
     2341 cgcctcgccg ctttggcgcc ggccgatctc gaccatgtct tcttcgccaa ttccggctcg
     2401 gaggcggcgg acacggcgct gaaaatcgcc atcgcctatc acaatgtccg cggccaaggc
     2461 gcgcgccagc gcctgatcgg ccgcgagcgc gcctatcacg gcgtcggctt cggcggcatt
     2521 tcggtcggcg gcatggtcaa caaccgcaag ttcttcggct cgcttctcgc cggcgtcgac
     2581 catttgccgg cgacgtacaa tcgcgagcgg caggccttca ccaagggcga gccggaatgg
     2641 ggcggccatc tcgccgacga gctcgagcgg atcgtcgcct tgcacgacgc ctcgaccatc
     2701 gccgcggtga tcgtggagcc gatggccggc tcgaccggcg tcttgccggc gccgaagggc
     2761 tacctgcaga agctgcgcgc gatctgcgac aaatacggca tcctgctcat cttcgacgag
     2821 gtgatcaccg gcttcggccg gctcggcgcg gctttcgccg ccgaacgcta cggcgtcatc
     2881 cccgacatca tcaccttcgc taagggcgtc acctccggca cggtgccgat gggcggcgtg
     2941 atcgtgcgca agcatgttca cgacgctttc atgaaagggc cggaacaggc gatcgagctg
     3001 ttccacggct acacctattc ggcccatccg ctcgcttgcg ccgcctcgct ggcgacgctc
     3061 gatacctatc gcgacgagaa gctgttcgag cgcgccaagg cgctcgagcc gaaatggtgc
     3121 gacgcgatca tgagcctgaa gggcctgccc aacgtgctcg acatacgctg cgtcggcctg
     3181 accggcgcga tcgacctcgc cccgtctggc ggcggcgccg gcgcgcgcgg ctacgccgcg
     3241 gtcgagcatt ccttccatga ggaaggaatc atgatccgca tttccggcga cacgatcgag
     3301 ctgaccccgc cgctgatcgt cagcgaggac cagatcggcg agatcgtcga caaggtcggc
     3361 aagacgatca aagcggtggc gtagggcgaa gggagcgccg cgccgattca ccgcggcgcc
     3421 gggggacgcc tctccccgcg aagcggggag aggtacggag aggggccggt cggaaaggct
     3481 cgatgcgcgg cattcgcatt cccgaaacga aacgggctcg cgcgctgcgg cgcgacgcca
     3541 gcgacgccga acgaaaatta tggcgggtgc tgcgagcccg ttcgctggat gggcgcaaat
     3601 tcgttcggca ggagcgaatt ggcccttact atgcggattt cgcttgtcgg gaagcgaaac
     3661 tggtcgtcga aatcgatggc gccactcatt cgactgagga agagcgtgcc agggatcggg
     3721 ggcgcaccgc cctgatgact cggcacggct atcgcgtcgt tcgctttaca aatatcgaag
     3781 tctatgagca gatcgaccgt gtgtgcgagg caatacttgt cgcgcttgct gagcgtcaga
     3841 gccctgtcga cccgcccctc tccgtacctc tccccgcttc gcggggagag gaggcctaag
     3901 cgccgagccc aactgtgatc gtcgagcgca gctcgcgccg tctctgaaat aatgctcctc
     3961 tatctcctcc agcgcctcct tctcctcctc gccaccttgg ctttcgcctc gctggtcgtg
     4021 ttcggcgtgc ttgaaatcct gccgggcaat gcggcgcagg tgatgcttgg cgcgagcgcg
     4081 acgccggagg cggtggcggc gctcgcccat aagctcggcc tcgatcagcc tttcgccgtg
     4141 cgctacgtcg cctggctgtc gggcatcgcc catggcgatc tcggcctttc ctacgcctat
     4201 aatacctcga tcggcgcgct gatcgccgac cgcctgtcgg tcagcgggcc gctggccctc
     4261 atcgccatga tcctgaccag cgtgatcgcg ctggccgcgg gcctcttcgc cgcgcagcgg
     4321 cgcggcaaaa acggcgattt cgcggtgatg gcggcgagcc agatcggcct cgccgtgccc
     4381 aatttctggt tcgcgatcct gctcgtcctg atcttcgcgg tgaagttgcg catcgcccat
     4441 tccgggggtt ttccgggctg gagcgccggt ttcggccaag cgctcggcgc cttgatcctg
     4501 ccggcgatct cgctcggcct ggtgcaggcg gcgatcctca cccgcgtcac ccgctcggcc
     4561 ttgctcgacg tgctcaacga ggacttcatc cgcacggcgc gggccaaggg cctgtcgcgc
     4621 cgcgccgcgc tttggcgcca cgccctgccc aacgccctga cgccgatcct gacgatcatg
     4681 ggcctgcaat tcgccaatct catcgccggc gcgatcatcg tcgaaaacgt cttcgtgctg
     4741 cccgggatcg gccaattgat cttccaggcg atcgccaatc gcgacattct cgtcgtcgaa
     4801 gattgcgtcc tgctgctcgc cgcgatcgtc atcgtcgtca atttcgtcgt ggacgtcgcc
     4861 tgcgcggcga tcgatcctcg cctcaggcgg cgcgctctat gagcaaggac ggtttccttt
     4921 cccgcgccaa gcgcaatccg agcttcctga tcggcgcggc cctggtgctg ctcgtcgcct
     4981 gcgccgccgc cttgtcgctg atctggacgc cgtatccgcc ggccgcgatc gatgtgcctc
     5041 ataaattgct gacgccgtcg gcgcagcatt ggctcggcac cgattcgctc ggccgcgacg
     5101 tcgcctccga gcttctcgcc ggcgcaagga tctcgatcct ggtcggggtg atcgcggtcg
     5161 gcatcggcct cgtcttcggc gtcgcgctcg gcctcttcgc cgcctcggcg aagggcctca
     5221 tcgaagacat cgtcatgcgc ttcagcgatt tcgccttcgc ctttccggcg ctgttgatgg
     5281 cgatcatgct gaccgcgaca ttcggcgccg gcgtcgtcaa cgcgatcatc gcgatcggca
     5341 ttttcaacgt gccgctcttc gcgcgggtca cccgcgcctc ggccaacacg gttttgtcgc
     5401 gcgaatatat cctcgccgcc cgcgccgccg gcaaaggccc gatcgccatc gccgtcgagc
     5461 acgtcctgcc gaatatcgcg ccgatcctga tcgtccaggc gacgatccag ttcgccatcg
     5521 cggttctcgc cgaggcggcg ctctcctatc tcggcctcgg cgcgcagccg ccgcagacct
     5581 cgtggggccg catgctcagc gaagcgcaga acctgatgta ccaagcgccg cgtctcgcca
     5641 tctttcccgg cctcgccatc gtcgtcgcgg tgctcggcct caatcttctc ggcgacggct
     5701 tgcgcgacct gctcgatccg cgcctgaggg cgaggcgcta agcatggttt ccctggttgg
     5761 cccgatgcgg cttaccctca ccccaaccct ctcccgcaag cgggagaggg ggcgcgccga
     5821 atgcgtcctt ggcgttggcg cctattacgg cgcttcggcc agcattcctt cttccgcttg
     5881 cgggagaagg tggcggcgaa gccgccggat gagggtaagc cgcggcgctg gtcggcgacg
     5941 cgccaaccga actctattta accatgttgc ttgaaatcgc cggcctcaat atcgacctgc
     6001 cgacgccgga cggcccgcgc cgcatcgtgc gcgacgtttc cttcagcctc gctcgcggcg
     6061 agacgctcgg cgtcgtcggc gaatccggct ccggcaagac catgaccgcg ctggcgctga
     6121 tgggcctctc cccggaaggc gccgctctta gcggcgcggt ccgcttcgac ggcgaagatc
     6181 tagccaggat cgacgaagcc gcgtggcggc gcctgcgcgg cgaccgcatc gcgatgatct
     6241 tccaggagcc gatgacggcg ctcaatccgt tgcagacgat cggccggcaa atcgccgagc
     6301 ccttgatcct gcatcgtggc ctcaggcgcg gcgcggcgat gcaggaagcg gtgaaattgc
     6361 tggcgcgcgt cggcctgccc gatccggcgc ggcgcgcccg cgcctacccg catcagctct
     6421 ccggcggcca gcgccagcgc gcgatgatcg ccatggcgct gagctgcggc ccgaagctgg
     6481 tcatcgccga cgagccgacc accgcgctcg acgtgaccgt gcaggcgcaa atcctttcgc
     6541 tgctcgccga attgcgcgag cagacggaca tggcgatgat cctggtcagc cacgacctcg
     6601 cggtgatcgc gcagaattgc cagcgcgtcg tcgtcatgta tggcggcatg gtgatggaga
     6661 gcggcgcgac ggccgacgtg ctggagcggc ccgcccatcc ctatacccgc gccttgctgc
     6721 aagcgcggcc gcggctcggc ggaacgcgcg gccagaggct gcgggtcatt ccgggctcgg
     6781 cgccgacggc gggagaaacg ccgaaaggct gccccttcgc cggccgctgt ccgctgacca
     6841 tcgccgcctg ctcggcggcg cccccgccgc cattcccggt cgggagcggc cgcatcgcgc
     6901 gctgcatccg cctcgaagaa gcggagaaag tggcgtgagc gagccgcttc tccaattgat
     6961 cgatctcgcc aagacgtacc gtttggcgag cgaaaagccg ttcgggccga aaaagctcct
     7021 tcgcgccgtc gacggcgtca gcctcgccat cgccgcgggc cgctccttcg gcattgtcgg
     7081 cgagtcgggc tccggcaaat cgactttggc gcgctgcgcc atcgcgctcg aaaagccgag
     7141 ctccggcgaa gcccggctga tgggccagcc gctcggaaaa gcttcgcgcc gcgaattgcg
     7201 cgccatgcgc gcccgtatgc agatgatctt ccaggaccct tacggctcgc tcgatccgcg
     7261 ccagacggtg gcgcggatca tcgccgagcc gcttgcggtg ctcgacggcg tgtcgcgcgc
     7321 cgagaccgcc aagcgcgtcg ccgaatcgct ggaggcggtc ggcctcggcg ctgccgacgg
     7381 caggaaatat ccgcatgaat tttccggcgg ccagcgccag cgcatcgcga tcgcgcgggc
     7441 cctgatcacc aggccgaaac tggtcgtcgc cgacgagccg gtctcggcgc tcgacgtctc
     7501 ggtgcaggcg caagtgctca acctgatgat ggatttgcag caaagcgccg gcgtcaccta
     7561 tcttttgatc agccacgacc tcgccgtggt cgagcatgtc tgcgaggaga tcgccgtcat
     7621 ctatcgcggc cgcttcgtcg agaccggacc gacggagaag ctgctcgccg cgccggctca
     7681 tccctatacg cgggcgctgc tcgacgccgc gcccaagctg aaagtcgagc gcccgcccgc
     7741 cgccgcgccc gacgccccga cggcgccgga atccggcgcg ggctgcgcct atgccgagcg
     7801 ctgccccttc gcgcaggagc gctgccgcgc cgaggcgccg gcgctcaggc cgctcgccga
     7861 cgggcgccag gcggcgtacc atttcccttt ggattgatca tcctcccccg cgaaagcggg
     7921 ggaccatcct cccccgtcga agacggggta tctgtttagc gccttatcgg ctttaagctt
     7981 gcgcggcgtg gggcggcgtg attcacgata aggatgaatc gcgcatatct tgattcgctg
     8041 gacaaagc
//
LOCUS       DATGJW010000518         2951 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1449230, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000518 DATGJW010000000
VERSION     DATGJW010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2951)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2951)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2951
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1449230"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1464)
                     /locus_tag="VKU03_15735"
     CDS             complement(<1..1464)
                     /locus_tag="VKU03_15735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012783841.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="homocysteine S-methyltransferase family protein"
                     /protein_id="HLJ72767.1"
                     /translation="MTDTLDALFPEADRADALAALTAAARERILIIDGAMGTQIQGLG
                     FEEGHFRGERFASCDCLLRGNNDLLTLTQPDAIEAIHLAYALAGADIIETNTFSSTAI
                     AQADYRMQEAVYDLNRDGARLARRAVLKAQAQDGRRRFVAGALGPTNRTASISPDVNN
                     PGFRAVTFDDLRLAYAEQVRGLIDGGADLILIETIFDTLNAKAAIFAAQEVFAEKGVV
                     LPLMISGTITDLSGRTLSGQTPTAFWHSLRHAKPFSIGLNCALGASAMRPHLAELADI
                     ADTLVCAYPNAGLPNAFGQYDESPEAMAAQLAGFAADGLVNIVGGCCGSTPEHIRRIA
                     EAVRGKAPRAIPQVPTYMRLSGLEPFTLTPDIPFVNIGERTNVTGSAKFRKLITAGDF
                     PGALAVARDQVANGAQIIDVNMDEGLIDGEKAMAEFLNLLAAEPDIARVPVMIDSSKF
                     SVIEAGLKCVQGKPLVNSISMKEGEEAFLAQARRVRAY"
     gene            complement(1596..>2951)
                     /locus_tag="VKU03_15740"
     CDS             complement(1596..>2951)
                     /locus_tag="VKU03_15740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009341280.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72768.1"
                     /translation="VVIALGVTWILDGLEVTLAGSIAGALKASPALEFSDQDVGLAGS
                     AYLIGAVSGSLFFGWLTDRLGRKKLFFITLTVYLLATAATGLSWNAATFFLFRFLTGA
                     GIGGEYTAINSAIQELVPARYRGATDLAINGSFWIGAAIGALAAIVLLDPKLINPELG
                     WRLAFLIGAAIGLVIILMRMWIPESPRWLIIHGRPEEAERIVSQIEARVKAGGGSVEP
                     LDAARSIKIRGRSHTPLREVLDTLLKTHLRRTLVGLTLMASQAFLYNAIFFTYALILT
                     EFFHVASQSIGWYILPFAVTNFLGPLLLGPLFDSRGRRFMISLTYALSGVLLLASGAL
                     FFADLLTATTITLAWMAVFFFASPAASSAYLTVSETFPVEIRAMAIAAFYAIGTGIGG
                     VAAPALFGALIQSGSRSSVFAGYVFASALMLGAAVVAALFAVKAERRPLEDVARPLSA
                     E"
BASE COUNT          551 a          900 c         1037 g          463 t
ORIGIN      
        1 ataggcgcgc acgcgccggg cctgagcgag gaaagcctct tcgccctcct tcatcgagat
       61 cgagttgacc agcggcttcc cctgcacgca tttgaggccg gcttcgatca ccgagaattt
      121 cgaggagtcg atcatcaccg gcacgcgggc gatgtccggc tcggcggcga gcagattgag
      181 gaactcggcc atcgcctttt cgccgtcgat cagcccctcg tccatattga cgtcgatgat
      241 ctgcgcgcca ttggcgacct ggtcgcgcgc caccgcgagg gcgccgggaa aatcgccggc
      301 ggtgatcaat ttgcggaatt tggccgagcc ggtgacattg gtgcgctcgc cgatattgac
      361 gaagggaatg tccggcgtca gcgtgaacgg ctccaggcct gacaggcgca tatatgtcgg
      421 gacttgcggg atcgcgcgcg gcgccttgcc gcgcaccgcc tcggcgattc tcctgatgtg
      481 ctccggcgtc gagccgcagc agccgccgac gatgttgacg aggccgtcgg cggcgaatcc
      541 ggcaagctgc gccgccatcg cttcggggct ctcgtcgtac tgaccgaacg cgttgggcag
      601 gccggcgttc ggataggcgc agacgagcgt atcggcgatg tcggcaagct cggcgaggtg
      661 cgggcgcatc gcgctcgcgc cgagcgcgca attgagcccg atcgagaagg gtttagcgtg
      721 gcgcagcgaa tgccagaagg cggtcggtgt ctggccggaa agggtgcggc cggaaagatc
      781 ggtgatcgtg ccggagatca tcagcggcag gacgacgcct ttctcggcga agacttcctg
      841 cgccgcgaag atcgccgcct tggcgttgag cgtgtcgaaa atcgtctcga tcaggatgag
      901 atcggcgccg ccgtcgatca ggccgcgcac ctgctcggcg taggcgaggc gcagatcgtc
      961 gaacgtgacg gcgcgaaagc ccggattgtt gacgtcgggc gagatcgagg cggtgcggtt
     1021 ggtcggcccg agcgcgccgg cgacgaagcg gcggcggccg tcttgcgcct gggccttgag
     1081 gacggcccgc ctggcgagcc gcgcgccgtc gcgattgagg tcgtagaccg cctcctgcat
     1141 ccggtaatcg gcctgggcga tcgcggtcga ggagaacgtg ttggtttcga taatgtcggc
     1201 gccggccagc gcataggcga ggtggatcgc ctcgatcgcg tcgggctggg tcagggtgag
     1261 caggtcgtta ttgccgcgca gcaggcagtc gcacgaagcg aagcgctcgc cccggaaatg
     1321 tccctcctcg aagccaagcc cttgaatctg cgtgcccatc gcgccgtcga taatgaggat
     1381 gcgctcgcgc gcggccgcgg tcagagcggc gagcgcgtcg gcgcggtcgg cctcagggaa
     1441 aagcgcgtcg agggtgtcgg tcattggaac tcgcttacgc agagcatcgc tgacttaatg
     1501 acataaagat atgtttatat caaggtgtca ttccgtcatt ccgggaatcc ggggagcccc
     1561 ggcgcgcgaa agctggcgcg gcgactgacg ggcgtctatt ccgccgaaag cggccttgcc
     1621 acgtcctcca acggccggcg ctcggctttg accgcgaata aggcggcgac caccgccgcg
     1681 ccgagcatta aggcggaggc gaagacatag ccggcgaaga cgctgcttcg cgagccgctc
     1741 tggatcagcg cgccgaacaa ggcgggcgcg gcgacgccgc cgatgccggt gccgatcgca
     1801 tagaaggcgg cgatcgccat ggcgcggatt tcgaccggga aggtctcgct caccgtgagg
     1861 tacgccgaac tcgccgccgg cgaagcgaag aagaacaccg ccatccaggc aagtgtgatg
     1921 gtcgtcgcgg tgaggagatc ggcgaagaac agcgcgccgc tcgccagcag caagacgccg
     1981 gaaagcgcgt aggtgagcga gatcatgaac cgacggccac ggctgtcgaa gagcggcccg
     2041 agcaggagcg gcccgaggaa attggtgacc gcgaagggca ggatgtacca gccgatgctt
     2101 tgtgaagcga catggaagaa ctcggtcagg atcaacgcat aggtgaagaa gatcgcgtta
     2161 tagagaaacg cctgcgacgc catcagcgtc agcccgacca gcgtacgtcg aaggtgcgtc
     2221 tttagcagcg tgtcgagaac ttcgcgcagc ggcgtgtgag agcgcccgcg aatcttgatg
     2281 ctgcgggccg catcgagcgg ttcgaccgag ccgccgccgg ctttcacgcg cgcttcgatc
     2341 tgactgacga tccgctccgc ctcctcgggt cggccatgga tgatcagcca gcgtgggctt
     2401 tccggaatcc acatccgcat cagaatgatg acgaggccga tcgccgcgcc gatcaggaag
     2461 gcgaggcgcc agccaagctc cggattgatg agcttgggat cgagcagcac gatcgccgcc
     2521 agcgcgccga tcgccgcgcc gatccagaag ctgccgttga tcgcaaggtc cgtcgcgccg
     2581 cgatagcgcg ccggcacgag ctcctggatc gccgaattga tcgccgtgta ttcgccgccg
     2641 attccggcgc cggtgagaaa gcggaaaagg aaaaacgtcg cggcgttcca ggaaaggccg
     2701 gtcgcggcgg tggcgagcag atagacggtc agcgtgatga agaacagctt cttgcggccg
     2761 aggcggtcgg tcagccagcc gaaaaacagc gagccgctca ccgcgccgat cagataggcg
     2821 ctcccggcaa ggccgacatc ctgatcggaa aattcgaggg cggggctcgc tttcagcgcg
     2881 ccggcgatcg agccggccag cgtcacttcg agcccgtcga gaatccaggt gacgccgagc
     2941 gcgatgacga c
//
LOCUS       DATGJW010000519         2042 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_828260, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000519 DATGJW010000000
VERSION     DATGJW010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2042)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2042)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2042
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828260"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..895)
                     /locus_tag="VKU03_15745"
     CDS             complement(<1..895)
                     /locus_tag="VKU03_15745"
                     /inference="COORDINATES: protein motif:HMM:NF015523.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MltA domain-containing protein"
                     /protein_id="HLJ72769.1"
                     /translation="MPAPHRLEPLAFHSLAGWTEDDHQAALGAFRRTGETLAAAKDSP
                     RPAQPPPPELISIAHAALDQASAAGRDARRFFETRFRPFRVTPAAGGGFLTGYYQPCV
                     PASPVETEQFRWPILKRPADLVSFAPGPAPAGFPEGVTGARRLSDGSLVPYPDRAAIE
                     AERRDAIAWVRDAAEAFLIQVQGSAELEFPDGRRARLAYDGRNGLPYTSIGKILIETG
                     EIAESDMALRSLKAWLRRDCARGLALMRRNRSFVFFRLVEDYDPSLGPVAGAGAPLTP
                     LRSIAIDRAIWAYGLPFWIDAE"
     gene            complement(900..1607)
                     /locus_tag="VKU03_15750"
     CDS             complement(900..1607)
                     /locus_tag="VKU03_15750"
                     /inference="COORDINATES: protein motif:HMM:NF033779.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Tim44/TimA family putative adaptor protein"
                     /protein_id="HLJ72770.1"
                     /translation="MPEIDLPTVIFALVALFVAWKLRSVLGMRQDSQRPGGLLAPLRR
                     VPGSPAPPVVQPDPAAPAPAPQPAAERWKGVAEPDPAVWTGLDAIAATDRGFSPEAFL
                     SGARVAYDMVVHAFAAGDSATLRNLMTPEAFANFDNAIRARAAARQTMTTTVVSIDNV
                     TIVGAHLIGAMAQLRARFAAKLVSATHDAQGAVVDGSPSTVEDHIDLWTFARDVRSRD
                     PNWQLTATESESAAPQG"
     gene            1741..>2042
                     /locus_tag="VKU03_15755"
     CDS             1741..>2042
                     /locus_tag="VKU03_15755"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013167600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-dehydropantoate 2-reductase N-terminal
                     domain-containing protein"
                     /protein_id="HLJ72771.1"
                     /translation="MRRANVIGVAGAGAWGVALANAAAAAGRTVILWGRDEAAMRAMA
                     KTRASGRLPGVALAGAVEATSDLERLRSCEAIFVATPAQSTRETALRLAAAPGSAPL"
BASE COUNT          312 a          683 c          769 g          278 t
ORIGIN      
        1 attcggcatc gatccagaac ggcaggccgt aggcccaaat cgcccggtcg atcgcgatcg
       61 agcgcaaggg cgtgagcggc gcgcccgcgc cggcgaccgg accaagcgag ggatcgtaat
      121 cctcgacaag cctgaagaac acgaacgaac gattgcgccg catcagcgcc agtcctcggg
      181 cgcagtcgcg gcgcagccac gccttcaggc tcctgagcgc catgtcgctt tcggcgatct
      241 cgcccgtttc gatcagaatc ttgccaatcg acgtgtaggg caggccgttg cgcccgtcat
      301 aggcgaggcg cgcgcgcctt ccgtccggaa actcgagctc ggccgagccc tgcacctgga
      361 tgaggaacgc ctcggccgcg tcgcgaaccc aagcgatcgc gtcgcgccgc tcggcctcga
      421 tcgccgcccg gtcggggtag gggacgagcg agccgtcgct caagcgcctc gcgcccgtga
      481 ccccttcggg aaagccggcc ggcgccgggc cgggagcgaa gctcacgaga tcggccgggc
      541 gcttgaggat cggccagcgg aattgctccg tttcgaccgg cgaagccggg acgcaaggct
      601 ggtaatagcc ggtgagaaac cctccgccag ccgcgggcgt gacgcggaag ggccggaacc
      661 gcgtctcgaa gaagcgccgc gcgtcccgcc cggcggcgct tgcctggtcc agcgccgcgt
      721 gggcgatcga gatgagttcg ggcggcggcg gttgcgccgg ccggggactg tcctttgccg
      781 ccgccagcgt ctcgcccgtc cgcctgaagg cgccgagagc cgcttgatgg tcgtcttcgg
      841 tccagccggc gaggctgtgg aaggcgagcg gttcgaggcg gtgcggcgcc ggcaagggct
      901 taaccttgcg gcgcggcgct ctccgattcg gtcgccgtca gctgccagtt gggatcgcgg
      961 gagcgaacgt cgcgcgcgaa tgtccacaga tcgatatgat cctcgaccgt gctgggcgac
     1021 ccgtcgacga ccgcgccttg agcgtcgtgc gtagcggaca cgagcttggc ggcgaaccgc
     1081 gccctgagct gggccatcgc gccgatgagg tgggcgccga cgatcgtgac gttgtcgatc
     1141 gacaccaccg tcgtggtcat cgtctggcgc gccgcggcgc gggcgcggat ggcgttgtcg
     1201 aaattggcga aggcttcggg cgtcatcaga ttgcgcaggg tcgccgaatc cccggcggcg
     1261 aaagcgtgga cgaccatgtc gtaggcgacc cgggcgccgg aaaggaaagc ttcgggcgag
     1321 aagccgcgat ccgttgctgc gatggcgtcg agaccggtcc agaccgcggg gtcaggctcg
     1381 gcgacgccct tccatcgttc cgcggcgggc tgcggcgccg gcgccggcgc cgccgggtcc
     1441 ggctggacca ccggaggagc gggcgatccg gggacacggc gtaagggggc caaaaggccg
     1501 cccggccgct gcgaatcctg gcgcatgccg agcaccgagc gcagcttcca ggcgacaaac
     1561 agggcgacaa gcgcgaatat gacggtcggg agatcgatct ccggcatcgc taccaagctc
     1621 ctcgctgcgc cctgcggcca agtccgccgc cggcgccgct ccggccataa ctaggcgcgg
     1681 ccaagcgttt tggctaggag catggtctga cgggcaaatg agaatgaggc gcgaatcggc
     1741 gtgaggcgcg caaatgtcat cggcgtcgcg ggagccggcg cctggggcgt ggcgctcgcc
     1801 aacgcggccg cggcggcggg ccggacggtg atcctttggg gccgcgacga ggcggcgatg
     1861 cgcgcgatgg ccaagacgcg ggcgagcggc cggctgccgg gcgtcgccct ggcgggcgcg
     1921 gtcgaggcga cgtcggatct cgagcgcctt cgcagctgcg aggcgatttt cgtggcgacg
     1981 ccggcccagt cgacccgcga gacggcgctc agactcgcgg ccgcgccggg atcggcgccg
     2041 ct
//
LOCUS       DATGJW010000520         2193 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_828649, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000520 DATGJW010000000
VERSION     DATGJW010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2193)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2193)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2193
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828649"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1426
                     /locus_tag="VKU03_15760"
     CDS             <1..1426
                     /locus_tag="VKU03_15760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72772.1"
                     /translation="VGLAFMLERIGAPDGLVRVLPPLLALSSVVLIGALMRTTRMAAF
                     YAADRVGSPLYIGGAFTAVAVGLVASVSVTAPSAPPFIGLAIGFVLAAMVVGPLGRRT
                     GASSLRDIVSARFDNPLFGFIFGLAYFITGLLITSVGYESAVQALGPLLNASRHDAII
                     ITAIIVLLIVAPGGLAGFFWVAAATAGMIAFVLLLPIGLRIMADVGAGAPMLGGGAEA
                     AARARDMLTQYKAWPLADPWLNEVGAALGVAILPPLSAGAFVGLTSYQATRVGVYSLV
                     VILVFAIAFALGTPLWRAENGAAAQSLFASGLLMAALICAAAGAQTASRAVSAHAVYG
                     REGVLASQRLARARGLTILAVAFSGWLAYRQVIVPEKSFFIAIAIALSILGPPLALSF
                     SARAQAAHAIAAIVASLATMAGLAYANAWAVSAAQAPTIALIGAVAGFAVGWGFSAFG
                     APRPENLEPGRSALFIDSPFDGGA"
     gene            complement(1437..>2193)
                     /gene="hisN"
                     /locus_tag="VKU03_15765"
     CDS             complement(1437..>2193)
                     /gene="hisN"
                     /locus_tag="VKU03_15765"
                     /EC_number="3.1.3.15"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007615123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004401 - histidinol-phosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="histidinol-phosphatase"
                     /protein_id="HLJ72773.1"
                     /translation="TILPFFRTALTVENKKTVGFDPVTAADRAAEQAIRALIRKNFPD
                     HGIVGEEFGAERTDAEYVWVLDPIDGTKAFISGLPTWGTLIGLTHRGRPVYGMMSQPF
                     TRERYFGDGASASCRGPSAAAPGVRGEGSAVRPLRVRSCASLASATLMTTTPKMFDET
                     EAPAYRRVEDKVRLARYGADCYAYCMLAAGYIDIVIEANLKPHDIVALVPIVQGAGGI
                     VSTWDGGDAAGGGRIVAAGDRRVYEEALQLLAV"
BASE COUNT          309 a          725 c          732 g          427 t
ORIGIN      
        1 cgtcggcttg gccttcatgt tggagcggat tggcgcgccc gatggtctgg ttcgcgttct
       61 ccccccgctc ttggcgctct cctcggtcgt tctcatcggc gcgctcatgc gcacgacgcg
      121 aatggctgcg ttttacgccg ccgaccgggt cgggtctccc ctatacatcg gcggcgcctt
      181 cacggcggtc gctgtcggcc tggtcgccag cgtcagtgtg acggcgccga gcgcgccgcc
      241 ctttatcggc ctggcgatcg gcttcgtttt ggcggcgatg gtcgtcggac ccttgggacg
      301 gcgaacgggc gcctcctcgc ttcgcgatat cgtctcggcg cgtttcgaca atccgctgtt
      361 cggatttatc ttcggcctcg cctatttcat cactggcctt ctcataacct cggtcggcta
      421 cgagagcgcc gtccaggcgc ttgggccgct cctcaacgcg tcgcgccatg atgcgatcat
      481 catcaccgcg atcatcgtcc tgttgatcgt cgcgcctggc ggtcttgctg gtttcttttg
      541 ggtcgccgcc gcgacggccg gcatgatcgc ctttgtactg cttctgccga tcggactgcg
      601 gatcatggcc gacgtcggcg caggcgcgcc catgcttggc ggcggggcgg aagctgccgc
      661 ccgcgcaagg gacatgctga cgcaatacaa agcatggccg ctcgccgatc cctggctcaa
      721 tgaagtcggc gccgcgctag gcgttgcgat ccttccgcct ctgtcggcgg gcgccttcgt
      781 cggtctgacg tcgtaccaag cgacccgtgt cggcgtttat agcctcgtcg tcatccttgt
      841 cttcgcgatt gctttcgcgc ttgggacgcc gttatggcgc gccgagaatg gggccgcggc
      901 gcaaagtctc ttcgccagcg gcctgctgat ggcggctctc atttgcgccg ctgccggcgc
      961 ccaaacggcc tcgcgcgccg tgagcgcgca tgcggtttat ggcagggaag gcgtgctcgc
     1021 gagccaaagg ctggcgcggg cgcgaggctt gacgatcctt gctgtcgcgt tcagcggctg
     1081 gctcgcttac cgacaagtga tcgtaccgga aaagtcgttc tttatcgcca tagcgatcgc
     1141 gctctccatc ctggggccgc cgctcgcctt gtctttctcg gcgcgcgcgc aagcggcgca
     1201 tgcgattgcc gcgatcgtcg cgagcctcgc gacgatggcc gggctcgcct acgccaatgc
     1261 ctgggctgtc agcgccgcgc aagcgccgac gatcgcgctg atcggagcag tcgcgggctt
     1321 cgccgtcggc tggggcttca gcgctttcgg cgcgccgcgg cccgaaaatc tcgagccggg
     1381 gcgttcagcg ctctttattg attcgccctt cgacggcggt gcgtgaagaa cgcgcatcag
     1441 acggcgagaa gctgcagcgc ctcctcatag acgcgccggt cgcccgcagc gacaatacgg
     1501 ccgccgcccg cggcgtcgcc gccatcccat gtcgagacaa tgccgccggc gccctggacg
     1561 attggtacga gcgcgacgat gtcgtgcggt ttgagattgg cttcgatcac gatgtcgata
     1621 tagccggcgg cgagcatgca ataggcgtag caatccgcgc cgtaccgcgc gaggcgaacc
     1681 ttgtcctcga cgcgccgata ggccggcgcc tcggtctcat cgaacatttt cggggtcgtc
     1741 gtcatcaagg tcgccgaagc gagcgacgcg caggaccgca cgcgcaaggg gcggacggcc
     1801 gagccttcgc cccggacccc cggcgccgcg gcggaaggac cgcggcagga tgcgctggcc
     1861 ccatcgccga aatagcgctc gcgggtgaaa ggctgcgaca tcatgccgta gaccggccgg
     1921 ccgcgatggg tgaggccgat caaagtgccc caagtgggca ggcctgaaat aaaggctttg
     1981 gtgccgtcga tcggatcgag cacccagaca tattcggcgt cggtgcgttc ggcgccgaac
     2041 tcctcgccga cgatgccgtg atcgggaaaa ttcttgcgga tgagcgcgcg gatggcctgc
     2101 tcggcggcgc ggtcggcggc ggtaaccgga tcgaagccga cggtcttctt gttctcgacg
     2161 gtcaacgccg tgcggaagaa tggcaggatt gtc
//
LOCUS       DATGJW010000521         6876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_929065, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000521 DATGJW010000000
VERSION     DATGJW010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6876
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929065"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..191)
                     /locus_tag="VKU03_15770"
     CDS             complement(<1..191)
                     /locus_tag="VKU03_15770"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008141440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72774.1"
                     /translation="MIRFDGRVAIVTGAGGGLGRSHALGLAARGAKVVVNDLGRGGEA
                     PDSALAVADEIEEAGGVAMA"
     gene            complement(188..688)
                     /locus_tag="VKU03_15775"
     CDS             complement(188..688)
                     /locus_tag="VKU03_15775"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014495943.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MaoC family dehydratase"
                     /protein_id="HLJ72775.1"
                     /translation="MSETSLKMTTIDDLIARIGTETGVSRWFLIDQARIDAFADVTED
                     RQFIHIDPEAAKATPFGGTIAHGFLTLSMLSALAQDALPRPQGVAMGVNYGFEKLRFV
                     APVPSGRRIRGRFHLLDVVRRGPNEVQMRNKATVEIEGGDKPALVAEWLTLYVMAPDH
                     AKADAK"
     gene            complement(685..1500)
                     /locus_tag="VKU03_15780"
     CDS             complement(685..1500)
                     /locus_tag="VKU03_15780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015315792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72776.1"
                     /translation="MFTLDDLFSVKGRVALVTGGATGIGRICAEALLAAGARVLIASR
                     KSDACEAVARELSAIGPCEGFGGSLASEDGVRALGGELRRRTDRLDILVNNAGTTWGA
                     PFETFEWKAWERVLAINVTGLFTLTRDLSPMLFRAATEARPANVINIGSVMGTITQAD
                     RSYSYSASKAAVHHLTRILAAEFAGRHVTVNAIAPGPFKSKMTAFSLDKAEGLAAAME
                     SVPMKRVGRPEDIAGTVLYLAGLGGAYTTGAIIPVDGGGAAYPPPPLFFREAE"
     gene            complement(1733..2836)
                     /locus_tag="VKU03_15785"
     CDS             complement(1733..2836)
                     /locus_tag="VKU03_15785"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ72777.1"
                     /translation="MLLTEEQRLLRDSARAAVAAKAPVALWRKLRAENDEPGFSRDFW
                     RDCAEAGWAGILIPEAYGGLDFGFVGAGLIAREMGRTLAASPFLSTAIMAASALKIGG
                     SDAQKTFWLPRIAAGEAVISVAVDEDARHNPARIAATATETPQGWRLSGAKTCALDGA
                     AANAFLLAAKAGGEAALYLVQGDVPGLSRVPRRLVDDRRVAALRFENIEAREARLAGG
                     GALIEQVLDAGRVVLAASLCGVAEEAFARTLSYLKQRRQFDRTIGSFQALQHRVAIMH
                     VDIENAWSATLKALRALDSGAADAPLQIAMAKAKAGEVGRTATAECLQLHGGIGMTDE
                     FDIGLFLKKARVEAELFGDPAYHADRVATMLGY"
     gene            complement(3054..4229)
                     /locus_tag="VKU03_15790"
     CDS             complement(3054..4229)
                     /locus_tag="VKU03_15790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013504619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ72778.1"
                     /translation="MTLDRGALRQWLEANCPAEMRQPIRGDDDICWGGRHFAFQSPAQ
                     KLWLEKMAGRGFTVPTWPKAYGGAGLNPEEAAILGEEMRRLHARPPLTSFGISMLGPA
                     LLKFGNEEQKRTHLPRIARGEIRWCQGYSEPGAGSDLASLRTKAEDRGDYFLVNGQKI
                     WTSYADKADWIFCLVRTDPAAKKQQGISFLLIDMATKGVSTKPIVLISGKSPFCETFF
                     DNVEVPKENLVGTLNRGWDIAKYLLTHERGAISGGGLNVFDNEALDKAARAAAKAAGG
                     RLPDPVLRADIARAAIDSYASAATVQRYMQEAAAGGELGARSAVLKYDGAELNKRRCE
                     LLMSLAGAEGLTFDGNFGPESELAQRWLRSKGNSIEGGTSEIMLDIVAKRILELPSA"
     gene            4387..4875
                     /locus_tag="VKU03_15795"
     CDS             4387..4875
                     /locus_tag="VKU03_15795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020174671.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72779.1"
                     /translation="MKRLALALAALIAFSASAQAAGYITPAEKRWAPFSAALPACDDP
                     GVLSWITSGFGGKESEYWNSPLQITGYDRIREIGFRSNGVAYIPRRYCIARAALNDSR
                     FHTVIYQIEAEQGFASFGDGVEWCVAGLDRNLAYAPGCSVLRPFVTRYLGDDEKALFE
                     RY"
     gene            4923..5714
                     /locus_tag="VKU03_15800"
     CDS             4923..5714
                     /locus_tag="VKU03_15800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003612474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease T2"
                     /protein_id="HLJ72780.1"
                     /translation="MKGDRFLRWAFAGLLALASASPAGAEGWGDFWRKRPPPAAPCVL
                     DECLNGGGTQNPPPASPGDRAAPRPGEIAPGAFDYYLLTLSWSPGFCDTGGDSKAPDQ
                     CEAGSGLGFVVHGLWPQYAHGYPQDCDGFSRPVSRSALALARGVFPDESLARYEWRKH
                     GTCTGLSPEGFFATVRRARDSIAVPEAFKAPRQEQSFAPRDIERAFIAVNPGLRFEAM
                     AVTCRSGELQDVRLCLTKDLRSFVNCPEVARRSCRAPAISVAPVR"
     gene            5711..6574
                     /gene="rlmJ"
                     /locus_tag="VKU03_15805"
     CDS             5711..6574
                     /gene="rlmJ"
                     /locus_tag="VKU03_15805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016920525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008649 - rRNA methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0070475 - rRNA base methylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="23S rRNA (adenine(2030)-N(6))-methyltransferase
                     RlmJ"
                     /protein_id="HLJ72781.1"
                     /translation="MNYRHAYHAGNFADVFKHALLTRILVYLTRKEAPLRFMDTHAGI
                     GRYDLDGAEARRSGEWRGGVGKLNGAAPPPEIAALIEPYLRAVGPLDAEGRPRLYPGS
                     PALAQTLLRPQDRLALCELHPEDRAALVANMGRDGRLAIVGLDGYVALNAWLPPKEKR
                     GLVLIDPPFEAPDESKRLVDALANALRKWPRGVYALWRPIKDARDDARFLNALAALGA
                     PNMLRLELGVGEAPSASAASVPLSKTGLLVVNPPHVFFEEARLLLPYLANLLARGDGA
                     SWRCDWLTPPN"
BASE COUNT         1058 a         2398 c         2296 g         1124 t
ORIGIN      
        1 gccatcgcga cgccgccggc ctcctcgatc tcgtcggcga cggcgagggc cgaatccggc
       61 gcctcgccgc cgcggccgag atcgttgacc acgactttgg cgccccgcgc ggcgagtccc
      121 agggcatggc tgcggccgag cccgccgccg gcgccggtga cgatcgcaac gcgcccatcg
      181 aatctgatca tttcgcatcc gccttcgcgt ggtcaggagc catcacataa agcgtcagcc
      241 attcggcgac cagagccggc ttgtcgccgc cctcgatctc gaccgtcgcc ttgttgcgca
      301 tctggacttc gttaggcccg cgccggacca cgtcgagcaa gtggaagcgg ccgcgaatgc
      361 gccggccgga cggcacgggg gcgacgaagc gcagcttctc gaacccgtaa ttgacgccca
      421 tcgcgacgcc ttgagggcgc ggcagcgcgt cctgcgcgag cgccgagagc atcgacaatg
      481 tcaaaaagcc atgcgcgatc gtgccgccga acggcgtcgc cttggccgcc tcgggatcga
      541 tatggatgaa ttgacgatcc tcggtcacgt cggcgaacgc gtcgatccgc gcctggtcga
      601 tgaggaacca gcgcgagacg ccggtctcgg tcccgatccg ggcgatcaga tcgtcgatcg
      661 tcgtcatctt caagctcgtc tcgctcattc ggcctcgcgg aaaaagagcg gcggcggcgg
      721 ataggccgcg ccgccgccgt cgacgggaat gatcgcgccg gtcgtatagg cgccgccaag
      781 accggcaagg tacagcacgg ttccggcaat gtcctcgggc cggccgacgc gcttcatcgg
      841 cacgctctcc atggcggcgg cgaggccctc cgctttgtcg agcgagaagg cggtcatctt
      901 ggacttgaac gggccgggcg cgatggcgtt gaccgtgaca tgccggccgg cgaattccgc
      961 ggccagaatg cgggtgaggt gatgcacggc ggccttcgag gcggaatagg aataggagcg
     1021 gtcggcttgc gtgatcgtcc ccatgaccga gccgatattg atcacgttgg cgggccgcgc
     1081 ctcggtcgcg gcgcggaaca gcatcgggga gagatcgcgc gtcagcgtga acaggccggt
     1141 gacattgatc gcgagcacgc gctcccaggc tttccattcg aaggtctcga acggcgcgcc
     1201 ccaggtcgtt ccggcattgt tgacgagaat gtcgagcctg tccgtgcggc ggcgcaattc
     1261 gccgccgagc gccctgacgc cgtcctcgct cgccagcgag ccgccgaagc cctcgcacgg
     1321 gccgatggcg gagagctccc tggcgacggc ctcgcaggcg tcgcttttgc gcgaggcgat
     1381 cagcacgcgc gcgccggccg caagcagagc ctcggcgcag atgcggccga tgccggtcgc
     1441 gccgccggtg acaagcgcga cgcggccctt gactgagaac aggtcgtcga gagtgaacaa
     1501 gcgcgctcct tacgagaccg aatacatttg ctcggtttca cgacccctga tcgtagcgct
     1561 taccctcatc cggcgcttcg cgccaccttc tcccgcaagc gggagaaggg tttgtagccg
     1621 cggtcgccga ccgcggctac agcgcggctg atggagaccg cggcgcgccc cctctcccgc
     1681 ttgcgggaga gggttggggt gagggcaagc cgccgcatgt ggaggcggcc tctcaatagc
     1741 cgagcatggt cgcgacccgg tcggcatggt acgccggatc gccgaacagc tccgcttcga
     1801 cccgggcctt cttcaaaaag agaccgatgt cgaattcgtc ggtcatgccg atgccgccat
     1861 gcaattgcag gcattccgcg gtcgcggtcc ggccgacctc gccggccttg gccttggcca
     1921 tggcgatctg cagcggcgcg tccgccgcgc cgctgtcgag cgcccgcagc gctttcagcg
     1981 tcgccgacca ggcgttctcg atgtccacat gcatgatcgc gacgcgatgc tgcagcgcct
     2041 ggaaactgcc gatcgtgcgg tcgaattgcc tgcgttgctt gagataggag agcgtgcggg
     2101 cgaaggcctc ctcggcgacg ccgcagagcg acgcggcgag aacaacgcgg ccggcatcga
     2161 ggacttgctc gatgagcgcg ccgccgccgg cgagcctcgc ctcgcgcgcc tcgatgttct
     2221 cgaagcgcag cgccgcgacg cggcggtcgt cgacgagccg gcgcggcacg cgcgacaggc
     2281 ccgggacgtc gccctggaca aggtacaacg cggcctcccc gcccgccttg gcggcgagca
     2341 gaaaagcgtt cgcggccgcg ccgtcgagcg cgcaagtctt ggcgccgctc agccgccagc
     2401 cttgcggcgt ctcggtcgcg gtcgcggcga tgcgcgccgg gttatgccgc gcgtcctcat
     2461 cgaccgcgac cgagatcacg gcctcgccgg cggcgatcct cggcagccag aaagtcttct
     2521 gcgcgtccga gccgcctatt ttcagggcgc tcgccgccat gatcgcggtc gagagaaacg
     2581 gcgaagccgc gagcgtgcgg cccatttcgc gagcgatcag gccggcgccg acaaagccga
     2641 aatcgaggcc gccataggct tcggggatca ggatcccggc ccagccggct tcggcgcaat
     2701 cgcgccagaa atcgcgcgaa aagccgggct cgtcgttttc cgcgcgcagc ttgcgccaca
     2761 gcgcgaccgg cgccttggcc gccacggccg cgcgggccga gtcgcgcaag aggcgctgct
     2821 cctcggtcag cagcatccgg ccgctccagc ggcgcgcctc ccttctcccc gcaaggcggg
     2881 gagaaggtgg cccgaaggga cggatgaggg gctgcgccgg acgcgaaggg tacgcgcttt
     2941 atgaagcgca aatcgcgtag cgcggcgccg cctttcatcg ggccgcttcg cggccacctt
     3001 ctccccgcgt tgcggggaga aggggagcac gcgctttttc tgtcgaaaca tcttcatgcg
     3061 ctcggcagct ccaggatgcg cttggcgacg atatcgagca tgatctccga cgtgccgccc
     3121 tcgatcgaat tgcccttgct gcgcagccag cgctgggcga gctcgctttc cgggccgaaa
     3181 ttgccgtcga aggtcaggcc ttccgctccg gcgagcgaca tcagcaactc gcagcggcgc
     3241 ttgttgagtt cggcgccgtc atatttgagc acggccgagc gcgcgccgag ctcgccgccg
     3301 gccgccgcct cctgcatgta cctttgcacc gtcgccgccg aagcgtagct gtcgatcgcg
     3361 gcgcgggcga tgtcggcgcg caggaccgga tcgggcagcc gcccgcccgc cgccttggcc
     3421 gcggcgcgcg cggccttgtc gagcgcctcg ttgtcgaaga cgttcaagcc gccgccgctg
     3481 atcgcgccgc gctcatgggt caacaaatat ttggcgatgt cccagccgcg attgagggtc
     3541 ccgaccaggt tttccttcgg cacttcgaca ttgtcgaaga aggtctcgca gaacggcgac
     3601 ttgccggaaa tcagcacgat cggcttggtc gagacgcctt tcgtcgccat atcgatgagg
     3661 aggaaggaaa tcccctgctg cttcttggcg gcgggatcgg tgcgcacgag gcagaaaatc
     3721 caatcggcct tgtcggcgta ggaggtccag attttctggc cattgaccag gaaatagtcg
     3781 ccgcgatcct cggccttggt gcgcagcgac gcgaggtcgg agccggcgcc cggctcggaa
     3841 tagccctggc accagcggat ttcgccgcgc gcgatgcggg gaagatgcgt gcgcttctgc
     3901 tcctcattgc cgaatttcag caaagccggg ccgagcatcg aaatgccgaa actggtcaag
     3961 ggcggccgcg catggagccg ccgcatctcc tcgccgagga tcgccgcctc ttccgggttg
     4021 aggccagcgc cgccatacgc cttcggccaa gtcgggacgg tgaagccgcg gccggccatc
     4081 ttttcgagcc acagcttctg cgccggcgat tggaacgcga aatggcggcc gccccagcag
     4141 atatcgtcgt cgccgcgaat cggctggcgc atttcggccg ggcaattggc ctccaaccat
     4201 tggcgcagcg cgccgcggtc gagcgtcatc ggcggtcact ccgaacaagc gagagcgcgc
     4261 cggcgctctc cgctccgcct caatgttcac gttggcggcg ggcttgtcga gccccttgag
     4321 cgccgcttga ccctcgcggc gccgcgtcgc attgtccggc tccttatccg ctcgggattc
     4381 gctccgatga aacgtctggc gctcgccctc gccgccttga tcgccttcag cgcctcggcc
     4441 caagcggccg gctacatcac gccggccgaa aagcgctggg cgccgttctc ggccgcattg
     4501 ccggcctgcg acgatcccgg cgtcctctcc tggatcacct ccggtttcgg cggcaaggag
     4561 agcgaatatt ggaattcgcc tttgcagatc accggctatg accggatccg cgagatcggc
     4621 ttccgctcca acggcgtcgc ctacatcccg cggcgctatt gcatcgcccg ggcggcgctg
     4681 aacgattccc gctttcacac ggtcatctac cagatcgagg ccgagcaggg cttcgccagt
     4741 ttcggcgacg gcgtcgaatg gtgcgtcgcc ggcctcgacc gcaatctcgc ctatgcgccg
     4801 ggctgcagcg tgctgaggcc attcgtgacg cgctatctcg gcgacgacga aaaggcgctg
     4861 ttcgagcgct actaagcgct tggcctgacg cgccgcgcga accggcgtcc aggagcgcga
     4921 gcgtgaaagg cgatcgtttc ctccgctggg cttttgcagg actgctggcg ctcgcttcgg
     4981 cttcgcccgc gggcgccgag ggctggggcg atttctggcg caagcggccg ccgccggccg
     5041 cgccctgcgt gctcgacgaa tgtctcaacg gcggcggaac gcaaaatccg ccgccggcct
     5101 cgcctggcga tcgcgccgcg ccgcggccgg gcgagatcgc gccgggcgcg ttcgactatt
     5161 acttgctcac cctatcctgg tcgcccggct tttgcgacac gggcggcgac tcgaaagcgc
     5221 cggaccaatg cgaggctgga tcgggcctcg gctttgtcgt gcacggcctc tggccgcaat
     5281 acgcccatgg ctatccgcag gattgcgacg gcttctcccg tccggtctcg cgttcggcgc
     5341 tggcgctggc gcgcggcgtc ttccccgacg agagtctcgc ccgctacgaa tggcgcaagc
     5401 acgggacctg caccggcctg agcccggaag gctttttcgc caccgtcagg cgcgcccgcg
     5461 attcgatcgc cgttcccgag gccttcaagg cgccgcgcca ggagcagagc tttgcgccgc
     5521 gcgacatcga gcgcgccttc atcgccgtca atcccggcct gcgcttcgag gcgatggcgg
     5581 tgacctgccg cagcggcgaa ttgcaagacg tgcgcctctg cctgacgaag gacctgcgca
     5641 gcttcgtcaa ttgtcccgag gtcgcgcgcc gctcctgccg cgcgcccgcg atcagcgtcg
     5701 ccccggtgcg gtgaactacc gccacgccta tcacgccggc aatttcgccg acgtcttcaa
     5761 gcacgcgctg ctgacgcgca ttcttgtcta tctgacgcgc aaagaggcgc ctctgcgctt
     5821 catggatacg catgcgggga tcggccgcta cgaccttgac ggcgcggagg cgcggcgctc
     5881 cggcgaatgg cgcggcggcg tcggcaagct gaacggcgcc gcgccgccgc ccgagatcgc
     5941 cgcgctgatc gaaccctatc ttcgcgcggt cgggccgctc gacgcggagg gccggccgcg
     6001 cctctatccc ggctcgcccg cgctcgccca gaccctgctc aggccgcagg accggctcgc
     6061 gctctgcgag cttcatccgg aggaccgcgc cgcgctcgtc gccaatatgg gccgcgacgg
     6121 gcggctcgcg atcgtcgggc ttgacggcta tgtcgccctc aacgcctggc tgccgcccaa
     6181 ggagaagcgc ggcctcgtcc tgatcgatcc gcccttcgaa gcgccggacg aatcgaaacg
     6241 cctggtcgac gcgctcgcca acgcgctgcg caaatggccg cgcggcgtct acgctctgtg
     6301 gcgaccgatc aaggacgcgc gggacgacgc gcgcttcctc aacgcgctcg ccgcgctcgg
     6361 cgcgccgaac atgctgcggc tggagctcgg cgtcggcgaa gcgccgtcgg cgagcgcggc
     6421 aagcgtcccg ctgtcgaaga cgggcctcct cgtcgtcaac ccgccgcacg tgtttttcga
     6481 ggaggcgagg cttctcctgc cttacctggc gaatctgctg gcgcgcggcg atggcgcgag
     6541 ctggcgctgc gactggctga ccccgccgaa ttagtagaag cagtacgaga cgctttgcgc
     6601 ggagcattcc cttctccccg cttcgcgcag ggctctccgg ggaaagtaag aggcgagtcg
     6661 catcgtcgtc gacgaagtct cgtgaaataa cacgatttcg agatttcctc gttgagtttc
     6721 cggcgacgag acccagaaaa taaacatgcg gacctcgggg ctgctcacaa actcttgcga
     6781 gcagtacgga tgcaacgatg acgcaaatcc cctacaccgc gctgccatcg ccgcggggcg
     6841 gcgtcaatcc caagccggct ttcgccggtc gcttcg
//
LOCUS       DATGJW010000522         6280 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_828860, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000522 DATGJW010000000
VERSION     DATGJW010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6280)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6280)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6280
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_828860"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..859)
                     /locus_tag="VKU03_15810"
     CDS             complement(<1..859)
                     /locus_tag="VKU03_15810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015927185.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72782.1"
                     /translation="MSERVLITGGAGFIGRRLTQNLLNRGYRVRILDSLVEQVHGARR
                     RPADLPVEAELIAGDVRDAEALRKALAGVDSVVHLAAEVGVGQSMYAIERYVSANDLG
                     AAALLEKLAQKPVRRIVVASSMSVYGEGLYVDGDGNPVEDAERKNAVSLSWNPVDASG
                     APLTAVATPEWKRPSLASVYALTKFVQEQMTLTVAAAYGMEGVALRLFNVYGPGQALS
                     NPYTGVLAIFASRLANDKPPLVFEDGRQRRDFVHVDDVAEAFAVALEHPKAPGEIYNI
                     GSGQDRSILQ"
     gene            1111..1347
                     /locus_tag="VKU03_15815"
     CDS             1111..1347
                     /locus_tag="VKU03_15815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72783.1"
                     /translation="MNVKSPGRAHGKKSLVGFTAARSGEAFVIHIVDDAAEVFEIEAT
                     RENVESIVTNLQSLIAEAESVPSERGTDDAGLQG"
     gene            complement(1360..2163)
                     /locus_tag="VKU03_15820"
     CDS             complement(1360..2163)
                     /locus_tag="VKU03_15820"
                     /inference="COORDINATES: protein motif:HMM:NF013117.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008381 - mechanosensitive monoatomic ion
                     channel activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mechanosensitive ion channel family protein"
                     /protein_id="HLJ72784.1"
                     /translation="MGRDARAARHHRYRDCRSRRADRGLGRACRRGIDDRGGGLDETA
                     RPALAAAARYGVLILALVAALSQLGVQTASLLAALGAAGLAIGLALQSTLSNIAAGIM
                     LLWLRPFRVGDYIEVVAGNAIAGTVKEIGLFASRLETFDGLYVFAPNSALWNFAVRNH
                     SRNIGRIICFTVSLPAGADVDRARDVLLGAVRPLAMERPRPEIFIDNLSAGAPLLTCR
                     FWAEHDKITEAQRRVLDAARRALETASPDLRPTHIVRTVPPDSDPSRLL"
     gene            2452..3546
                     /locus_tag="VKU03_15825"
     CDS             2452..3546
                     /locus_tag="VKU03_15825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012605342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucuronic acid decarboxylase family
                     protein"
                     /protein_id="HLJ72785.1"
                     /translation="MGKVRKTCVVAGGAGFLGSHLCDALLSDGSEVVCVDNFRTGRID
                     NMRQLRREPRLVIVEADVINPLSSLLPRNVRRVDWVFNLACAASPPHYQSDPEHTMLT
                     NVIGTRNLLALSERMGARFLLASTSEVYGDPQVHPQPESYWGYVNPTGPRACYDEGKR
                     AAEALVFDYGRLGRGGIRVARIFNTYGPRMRADDGRVVSNVVTQALAGDDITVYGSGE
                     QTRSFCYVDDLIDGLLRLMAYEGDYAGAINLGNPAEITVRELVSRVLRLAGSESQIVR
                     RPLPVDDPQRRRPDIALATRLLGWTPRVSLERGLKKTIAYFAAEAAAVALPRTRGVGR
                     TKIGANGASGFLSKMKDAAVPGAERTLIRP"
     gene            complement(3478..4392)
                     /locus_tag="VKU03_15830"
     CDS             complement(3478..4392)
                     /locus_tag="VKU03_15830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLJ72786.1"
                     /translation="MTLDPRPLANAALRDVLASAAPPAIALMRLALASPNEAAFAAAV
                     GAIRQSAKDRRGGQEAERLAALVALADGAPDAWALVRALASVVPHAAREEGSMADRLA
                     ELAAAFDRAATLSPAASVALYSLGRPELLDAATADVVGYLETLGVIGPDRALLDIGCG
                     VGRFERALAGKVARIVGLDISPAMVEIARRDCAGLANIDIRLCSGADLSGVADASMDC
                     ALAVDAFPYVVLAAGGLPRRLFEEMARVLKPGGDVLIANYSYRADLAADPSDVARLAH
                     LNGLVVLRSDERPFRSWDGRVFHLGKKA"
     gene            complement(4382..5377)
                     /locus_tag="VKU03_15835"
     CDS             complement(4382..5377)
                     /locus_tag="VKU03_15835"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015319161.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ72787.1"
                     /translation="MSGLSIRAIDDLDGLAALESAWWDLWRRTPTATPFQSPAWLIAW
                     RRIFAPGPLRVGAVFDGGQLVALFPFYEEKNAAGSRLLPLGISVSDYLDILADPARPE
                     ELQSLAAFIAETPWRCCSFEELPPSAIARSLPPPSECSDDIDRHSACPVLVLRGGRDL
                     VGCVPARRRRQLRRARAAAARLGEVQIERADHDSDRFLAFAFSLHAARWRERGESGVL
                     SDEQVRRFHRTALPQLAAAGLARCYLLAIGGRNVAAYYGFLDRGRAFAYLGGFDPAFE
                     DASPGAILIGHAIEKAIDEDAREFHFLRGREAYKYSWGATDRWNQRRLWTRRDDA"
     gene            complement(5386..>6280)
                     /locus_tag="VKU03_15840"
     CDS             complement(5386..>6280)
                     /locus_tag="VKU03_15840"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008840579.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HLJ72788.1"
                     /translation="AGVHLPVESERGPLDDLARRRAWARHRRAINNARDCFPVDLVHL
                     HGVDFGAYCPASGATLVTLHLPLSWYDPNSLRPARADLWLNGVSQSQTRSAPAEARLL
                     PPIDNGVDVEALAARCGKRRFVLFLGRLCPEKGAHIAIAAARRASLPLIIAGQTFRYA
                     EHERYFAEEIRPHLGPACRFAGPLNFARKRRFLTAARCVALPSLVPETSSLVAMEALA
                     CGTPVVAFRAGALADIVAHGRTGFLVETAEEMAEAMIEAAALDPDTCREEARARFSNR
                     RMTAAYLDLYARIACKQALRA"
BASE COUNT         1120 a         2000 c         2167 g          993 t
ORIGIN      
        1 tttgcaggat gctgcggtcc tgcccgctgc cgatgttgta aatctcgccg ggagctttcg
       61 gatgctccag cgcgacagcg aaggcttcgg cgacatcgtc gacatgcacg aaatcgcggc
      121 gctggcgtcc gtcctcgaag acgagcggcg gcttatcatt ggcgagacgc gacgcgaaga
      181 tcgccaggac gcccgtatag ggatttgaaa gcgcctggcc gggcccatag acgttgaaca
      241 gtctcagcgc gacgccttcc attccatagg cggcggcgac ggtgagcgtc atctgctcct
      301 gaacgaactt ggtcagcgca tagacggagg cgagactggg ccgcttccat tccggcgtcg
      361 caaccgccgt cagcggcgcg cccgacgcgt cgaccggatt ccaggaaagg ctgacggcgt
      421 ttttgcgctc ggcgtcctcc accggattgc cgtcgccgtc gacatagagg ccctcgccat
      481 agacgctcat cgaggaggcg acgacaatgc ggcgcaccgg cttttgcgca agcttttcaa
      541 gaagcgcggc cgcgccgaga tcgttggctg agacgtagcg ctcgatggcg tacatgctct
      601 ggccgacgcc gacttcggcg gcgaggtgca cgacgctgtc gacgccggcc agcgctttcc
      661 gcagcgcctc ggcgtcgcgc acgtcgccgg cgatcaattc ggcctccaca ggaagatcgg
      721 cgggccggcg ccgggcgcca tgcacttgtt cgaccaggct gtcgagaatg cgaacgcgat
      781 acccgcgatt gagaagattt tgcgtcaggc gccgtccgat aaagccggcg ccgcccgtaa
      841 tgagaacgcg ttcagacacc gccgctcctg tggttccgcc gaatgcagaa aaacgtgtcg
      901 cgcttgggcg tgttccccgc ggccaaagga aattctcggg ggatttctgt tgaacgcacc
      961 ggcggttgaa aaagcgcggc gttcacagct cgcgccgccg agcgcgcgcc ggggaacttt
     1021 gccgccgccc ttccgttctg tcggccgaac gcggcccccg gtttcgcttc ggcttggcgt
     1081 cagcggcaag gagtggagct tctgctggaa atgaacgtca aatctccagg cagggcgcac
     1141 ggcaagaagt cgctcgtcgg gttcacggcg gcgcgcagcg gcgaggcttt cgtcattcac
     1201 atcgtcgacg acgcggccga ggtttttgaa atcgaggcga cgagagaaaa cgtggagtcg
     1261 atcgtcacga atttgcaatc gctgatcgcc gaagccgaat ctgtgccttc cgagcgcggc
     1321 actgacgacg ccggtcttca aggttaagca tcacgcgcgt cacaagagcc gcgacggatc
     1381 ggaatcgggc ggcacggtac gcacgatatg ggtgggcctg aggtcgggcg aagccgtttc
     1441 cagggctcgg cgagcggcgt cgagaacgcg gcgctgcgct tccgtgattt tgtcgtgttc
     1501 ggcccagaag cggcaggtca aaagaggcgc gccggcgctg agattgtcaa tgaagatttc
     1561 cggccgcggc cgttccatgg cgagaggccg aacggcgcca aggagaacgt cgcgcgcccg
     1621 gtcgacatcg gcgcccgccg gcaggctgac agtgaagcaa atgatccggc cgatattgcg
     1681 cgaatggttg cgcaccgcga aattccacaa cgcagagttc ggtgcgaaaa cgtacaagcc
     1741 gtcgaatgtt tcgagacggg aggcgaaaag accgatctcc ttaaccgttc cggcgatggc
     1801 gtttccggcg acgacctcga tatagtcgcc gacccggaat ggccgcagcc agagcagcat
     1861 gatgcccgcg gcgatgtttg acagcgtgct ctgaagcgct aggccgatcg ccaagccggc
     1921 ggcgccgagc gccgccagga gcgaagccgt ctggacgccg agttggctca gcgccgcgac
     1981 gagagcgagg atgagaacgc cgtagcgcgc cgccgcggcg agcgccgggc gcgccgtctc
     2041 gtcgaggccg ccgcctcgat catcgatgcc gcgacggcac gcgcggccca gcccgcgatc
     2101 agcacgccga gaacgacaat cacgatagcg atgatggcgc gcggcgcgag cgtcgcggcc
     2161 catgtccaaa gcgcggcggc gatagcgtcc ggatagttcg gaaggatagt catagccgtc
     2221 atgagcggga acttcatgac ggccgcgtcc tcttcgtgat tgcggccgcc ttatcgcgcg
     2281 atgaacacgg cgaagccaca attcgttcca tccgctgtgc tgcggagcgc tcctaggagg
     2341 cggggaaagc ccggaacttt cgtgtttttc gcctgttgtc ggctggtcga tactcgttca
     2401 atcccagttt cgcatggcga gccggggccc tccagaagaa aggtggacgg cgtgggaaaa
     2461 gtaagaaaga cctgtgtcgt cgccggcggc gccggctttc tcggttcgca tctttgcgac
     2521 gccttgcttt cagacggatc cgaagtcgtt tgcgtcgata attttcgcac ggggcgcatc
     2581 gacaatatgc gacagctccg tcgggagccg cgcctggtca tcgtcgaagc ggatgtgatc
     2641 aacccgctct cgtcactgct gccgcgaaac gtgcgacggg tcgattgggt cttcaatctc
     2701 gcctgcgccg cctcgccgcc gcattatcaa tccgatcccg aacatacgat gctcacgaac
     2761 gtgatcggga cgcggaactt gctggcctta agcgagcgga tgggcgcccg ttttctgctc
     2821 gcatcgacca gcgaggttta tggcgatccg caggttcatc cgcagccgga gagttattgg
     2881 ggctatgtta atccgaccgg cccgcgcgct tgctacgatg aaggcaagcg cgccgccgag
     2941 gcgctggtct tcgactacgg ccggctgggc cgcggcggga tcagggttgc gcgcattttc
     3001 aacacgtacg gaccgcgcat gcgcgccgac gacggccgcg tcgtctccaa cgtggtcacg
     3061 caggctttgg ccggcgacga catcacggtt tacggctccg gcgagcagac gcgctcgttt
     3121 tgctatgtcg atgatctgat cgacggcttg ttacgcttga tggcttatga aggcgattac
     3181 gccggcgcga tcaatctcgg caaccccgcc gaaatcactg tgcgcgaatt ggtctcccgg
     3241 gttttgcgcc ttgccggatc ggagtcccaa attgtccggc ggccgctgcc cgtcgacgat
     3301 ccgcagcgtc ggcggcccga tatcgcgctg gcgacgcgcc tgctcggctg gacgccgcgc
     3361 gtctccttgg agcgcggttt gaaaaagacg atcgcctatt tcgccgctga agccgcggcc
     3421 gtggcgctgc cgagaacgcg cggcgttggc cggacgaaaa tcggcgccaa cggcgcgtca
     3481 ggctttcttt ccaagatgaa agacgcggcc gtcccaggag cggaacggac gctcatccga
     3541 ccttagcacg acaagtccgt tcaagtgcgc caagcgggcg acgtcggagg ggtcggccgc
     3601 aaggtcggcg cgataggaat aattggcgat cagcacgtct ccgccgggct tcagaacccg
     3661 cgccatctcc tcgaagagcc ggcgaggaag acctccagcg gctaagacga cgtacggaaa
     3721 ggcgtccacg gcgagggcgc agtccatgct cgcgtcggcg acgccggaca ggtccgcgcc
     3781 cgagcacagc cgaatatcga tattggcgag gccggcgcaa tcccgcctcg cgatctcgac
     3841 catggccggc gagatatcaa ggccgacgat cctcgcgact ttgccggcga gcgcgcgttc
     3901 gaagcggccg acgccgcagc cgatatcgag cagcgcgcga tctggtccga tcacgccgag
     3961 ggtttcgaga tagccgacga cgtcggccgt ggcggcgtcg agcagttcag gccggccgag
     4021 cgaataaagc gcgacgctgg cggccgggga gagagttgct gcgcgatcga acgccgccgc
     4081 cagctcggcc agccggtccg ccattgagcc ctcttcgcgc gcggcgtgcg ggaccacgct
     4141 cgcgagcgcg cgcaccagcg cccaagcgtc cggcgcgccg tcggcgaggg cgacgagcgc
     4201 ggcgaggcgc tcggcctctt ggccgccgcg ccggtccttc gccgattggc ggatcgcgcc
     4261 gaccgccgcc gcgaaggcgg cctcgttcgg actcgccaag gcgaggcgca tcagggcgat
     4321 ggccggcggg gccgcgctcg ccagaacgtc gcgcagcgcc gcatttgcca aggggcgcgg
     4381 gtcaagcgtc atcgcgtcgt gtccaaaggc ggcgctgatt ccagcggtcg gtcgcgcccc
     4441 aggaatattt gtacgcttcg cggccgcgca ggaaatggaa ttcccgcgcg tcttcgtcga
     4501 tcgctttctc gatggcgtga ccgatcagaa ttgcgcctgg acttgcgtct tcgaaagccg
     4561 ggtcgaagcc gccaagataa gcgaaggcgc ggccgcgatc gagaaagccg taataggcgg
     4621 cgacgttccg cccgccaatg gcgagaaggt agcagcgcgc aagaccagcc gcggcgagct
     4681 gcggcagagc ggttcggtgg aagcggcgaa cctgctcgtc gctgagtacg ccgctctcac
     4741 cgcgctctcg ccaccgcgcg gcgtgcagtg agaaggcaaa ggcgagaaag cggtcgctgt
     4801 cgtgatccgc gcgctcgatt tgcacctcgc cgaggcgagc ggcggcggcg cgggctcggc
     4861 gcaattgcct gcgccggcgc gccggcacgc aaccaacaag gtctcgtccg ccgcgcagaa
     4921 caagaaccgg acaggcgctg tggcggtcga tgtcgtccga gcactcgctc ggcggcggta
     4981 gggacctcgc aatcgccgag ggcggcagct cctcgaagct gcagcagcgc catggcgtct
     5041 cggcgataaa ggcggcaagg ctttgcagtt cctccggccg cgccggatcg gcgaggatgt
     5101 cgaggtaatc gctgacggaa attccgagcg gcaggagccg ggatccggcc gcgttctttt
     5161 cctcgtaaaa cgggaagagc gcgacgagtt gtccgccgtc aaaaacggcg ccgacccgca
     5221 aggggccggg cgcgaaaata cgccgccagg cgatcagcca ggccggcgat tggaacggcg
     5281 tcgcggtcgg cgtccgccgc cataagtccc accaggcgga ttcgagcgcc gccagcccgt
     5341 cgagatcgtc gatcgcccgt atcgagaggc cggacatcgt cgcgctcagg cccggagagc
     5401 ctgcttgcag gcgatccggg cgtagagatc aagataggcc gcggtcatgc ggcgattgga
     5461 aaagcgcgcg cgcgcctcct ctcgacacgt atcggggtcg agcgcggccg cttcgatcat
     5521 cgcctcggcc atctcctccg ccgtctcgac aaggaagccg gttcgtccgt gcgcgacgat
     5581 gtcggccagc gcgccggcgc ggaaggcgac caccggcgtt ccgcaggcca gagcctccat
     5641 ggcgacgagc gagctcgtct ccgggacgag gctcggaagc gcgacgcacc gcgcggcagt
     5701 gaggaagcgg cgcttgcgcg cgaaattcag aggaccggcg aagcggcagg cggggccgag
     5761 atgcggccgg atttcctcgg caaaatagcg ctcgtgctcg gcgtagcgga aggtttggcc
     5821 ggcgatgatc aacggaagcg aagcgcgcct cgccgcggcg atggcgatat gagcgccttt
     5881 ttccgggcac agccggccga ggaaaaggac gaaccgcctc ttgccgcagc gcgcggcgag
     5941 cgcttcgaca tcgacgccgt tgtcgatcgg cgggagcagg cgcgcttcgg ccggagcgct
     6001 ccgagtctgg ctttgcgaaa cgccgttgag ccagagatcg gcgcgggccg ggcgcagaga
     6061 gttcggatcg taccaggaga gcggcagatg cagggtgacg agcgtcgcgc cgctcgcggg
     6121 gcagtaagcg ccgaaatcga cgccatgcag atgcacgaga tcgaccggga agcaatcgcg
     6181 cgcattgttg atggcgcggc ggtgccgcgc ccaagcccgc cgccgcgcca gatcgtcaag
     6241 cgggccgcgc tcgctctcga ccggcaggtg aacgccggcg
//
LOCUS       DATGJW010000523         9400 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1237305, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000523 DATGJW010000000
VERSION     DATGJW010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9400)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9400)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9400
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1237305"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(96..503)
                     /locus_tag="VKU03_15845"
     CDS             complement(96..503)
                     /locus_tag="VKU03_15845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015888413.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system VapC family
                     toxin"
                     /protein_id="HLJ72789.1"
                     /translation="MTFVDTNILIDIASNNSRWGDWSRRALAAAHERGPLVINVVVYA
                     GFAINFATAAECDAELESFQLTIADIPKAAAFGAAQAFKEYRLAGGARTNVLPDFFIG
                     AHALSLGAPLLTRDCRRYRTYFPKLTLLAPDAS"
     gene            complement(500..748)
                     /locus_tag="VKU03_15850"
     CDS             complement(500..748)
                     /locus_tag="VKU03_15850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011663468.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HLJ72790.1"
                     /translation="MTTTVTVKGQVTLPKAVREAAGIRPGDRVTVRVRPEGGVIVESA
                     ANHAGPHAYLQRLEAISRRKPIRNLTTEEVMAMTRNED"
     gene            complement(930..1046)
                     /locus_tag="VKU03_15855"
     CDS             complement(930..1046)
                     /locus_tag="VKU03_15855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AbrB/MazE/SpoVT family DNA-binding
                     domain-containing protein"
                     /protein_id="HLJ72791.1"
                     /translation="MATIVTVKGQVTLPKAAREAAGFRPGDRVVVRAGRKEA"
     gene            1210..2535
                     /locus_tag="VKU03_15860"
     CDS             1210..2535
                     /locus_tag="VKU03_15860"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008195845.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLJ72792.1"
                     /translation="MIHETDVLVVGSGPGGLAAALAAARAGVEVALIERFGCFGGNIT
                     TVGVEGFAWYRHAATIEAGGIGREFEERAKAMGAAVPESQSLSYELDSEGFKLVADRL
                     VEEAGVRPMLHRLFVAPIVDGDAVTGIIAESKAGREAILAKRVIDATGDADIAHRAGA
                     PTNKTPVEEMQAASVMFHLAGIDKRAFLEGVRADPQTYGDWSTGEWTVETSGKEDKMF
                     SPFLAKPFAEAIKAGLIPARLNTIGGTWGAVHDTGELTYMNLIHLAGCDGTDPDSLTR
                     FEIEGRRQAMLAIEALRRFTPGCAGARLRNFGMTIGIRDTRKIDALYNLTEQDVRNEA
                     RFEDSIGVYPEFIDGYGVLILPTTGRYMHIPYRALLPKQVENLLVAGRAIGGDRIAHA
                     ATRNMACCAVAGQGAGIAAALSVKTGRSPREVSLAAIHRELDRQGVRFQ"
     gene            complement(2644..3519)
                     /locus_tag="VKU03_15865"
     CDS             complement(2644..3519)
                     /locus_tag="VKU03_15865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72793.1"
                     /translation="MRNLVVLALLAAASPAFASGSEQAYLAARDHAVATLKHMKGDKQ
                     DAEERRLRAGLEAQLKSVINPPPLKDFPGPGTISPESLIQTDIGADGLDGLVFRNGDE
                     NTVLVTTDGLLKAWLKAHRNYWKDLPNPPVEPEAAFHSEAFYTQAASPDAAVSIFAML
                     PIRKPEGAATAAALLIQQAQDVAVDPPEQIAVAVAKGGRVYVAIVKAAAKPAPIAACA
                     AIWKDFQARSEAAAKAYQQAGKDDPKSADESTRLENEGAAAFHKCWSEKAGATAAFPA
                     LTQQAQSLVDTFAAQ"
     gene            3784..4956
                     /locus_tag="VKU03_15870"
     CDS             3784..4956
                     /locus_tag="VKU03_15870"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72794.1"
                     /translation="MNESISARASGWPVWAPIAAMFFVNGAVYGVWATQIPLAKLRLG
                     LDPSQLGATLFVLGLGAIAAMAASGWILQRIGAAALIRISGGVFCILLPLVGSAPSAR
                     ALAGLLFFFGASGGLMDVAMNVAAAEAERRAKRPYMSSFHGMWSLAGLTGAGGGTLLL
                     NVVSGPWQSFVMAALLAAVFVLGQARLRGEPEQRAEAAARRPALRPTLAALLVGLMAA
                     LCFSGEGAVLDWAAVYLRDSLGAAIERANLGYAAFSGAMAFGRFFGDRIRRSVDGPAL
                     VRGGCVLVFIGLLAGPLSNNFWIAVAGYLLTGLGFSNMVPVLFSAAGAMPHPETQIAA
                     VSTLGYAGLLAAPPLFGFVAQATSLAGIFYLVAAATILIAALAGVCAPARRVSPQS"
     gene            5067..6809
                     /locus_tag="VKU03_15875"
     CDS             5067..6809
                     /locus_tag="VKU03_15875"
                     /EC_number="6.3.5.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009339157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003952 - NAD+ synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD+ synthase"
                     /protein_id="HLJ72795.1"
                     /translation="MPAPADRLVIALGQLDATIGDIEGNAQRLRQARAEAAAFGADIL
                     MSPELFLSGYPPEDLALKPAFQEACRAACEALARETADGGPALLVGLPWAENGALYNA
                     YALLDGGRIEAVRFKVDLPNYGVFDEKRVFKPGPAPGPIVFRGVRIGVPICEDIWGPE
                     PVECIAETGGEILLVPNASPYERDKVGTRLNIAVNRVVESGLPLIYLNQVGGQDELVF
                     EGASFALNADRSLACQMPAFRQLVARTVWERQGETWCCVEAPREIVEEGDEADYAACV
                     LGLRDYVEKNRFPGIALGLSGGVDSALCAAMAVDAVGAARVHAIMLPYRFTSGESLND
                     AAACAKALNIRYDTIPIAGPVETIEKALQPLFAGRIRDVTEENIQSRARGLILMSVSN
                     KLGAMLATTGNKSELSVGYATLYGDMNGGFNPLKDLYKTQVFRLAQLRNRWKPFGALG
                     PDGEVIPTNILTKAPSAELRENQKDEDSLPPYAVLDAILEGLVEREMRVADIVAQGYD
                     AETVKKVERLLYLAEYKRRQSAPGVKVTSKSFGRDRRYPIVNRFRDSGEPKREPDAAI
                     APARPIGTSERFED"
     gene            complement(6806..7306)
                     /locus_tag="VKU03_15880"
     CDS             complement(6806..7306)
                     /locus_tag="VKU03_15880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013894267.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HLJ72796.1"
                     /translation="MTLAAPAPLANRHDLTRFDCGNASLDDWLKRRARANQISGATRT
                     FVACDDNVVAGYYALAAGGVSLAAATGRFRRNMPDPIPVALLARLAVDHRWQSKGIGR
                     ALFRDAAMRLASAAETLGIRGVLVHAISDEAKAFYLSLGFDPSPLEPMMLMATMADIQ
                     NVLGLT"
     gene            complement(7303..7569)
                     /locus_tag="VKU03_15885"
     CDS             complement(7303..7569)
                     /locus_tag="VKU03_15885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HLJ72797.1"
                     /translation="MARDTLNIRIRAEERGLIDRAAAALGKTRTDFMLEAAKRAAEEA
                     LLDRAVFSANPAAYAKFMERLDAPPRPNDRLRRALTTKAPWDAS"
     gene            complement(7714..8490)
                     /locus_tag="VKU03_15890"
     CDS             complement(7714..8490)
                     /locus_tag="VKU03_15890"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002717246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HLJ72798.1"
                     /translation="MVRNARIDAFDHVPRPVIAIGNDYPSGHVVLPHRHRRSQLLYGA
                     SGAVIFAAEQSAWVMPPQGGMWIPAGVTHEVRMLGAFSARSLYLEPEVAQGMPDHCQV
                     VAISPFMRSLLAEAVDLPADYDLGGRGGALMALVLHEIRRLPALPLSLPFPTHGALAE
                     RCRAFLEKPSPHATIDAWSEALGVSRRTFTRLIKRETGLSFALWRQRACLVAALPRLT
                     AGEKVTEVALDLGYDNPAAFTTMFKRVLGASPRNYLRENG"
     gene            8619..>9400
                     /locus_tag="VKU03_15895"
     CDS             8619..>9400
                     /locus_tag="VKU03_15895"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018266352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72799.1"
                     /translation="MSQAVIAQSKTGATAFSILGAISFCHLLNDMMQALLPAIYPILK
                     GGFDLSFGQIGLLTLTYQITASLLQPLVGLYTDRRPQPYSLPFGMGSTLLGLVALAFA
                     PNYLTLVAGSALLGVGSSIFHPESSRIARLASGGAHGLAQSLFQVGGNFGQSLGPLLA
                     AFVILPHGRASLAWFALAALGGMAILAALGNWSKRKGAARRSAHKLAPERSALPKARV
                     ARAVAVLIALIFSKYFYLASITSYYIFYLMRRFDLPTQTAQV"
BASE COUNT         1533 a         3089 c         3087 g         1691 t
ORIGIN      
        1 gcggggagag gaggccctgg cgctcagcct agatttcgac ccaccctggc ttcaatcagc
       61 gctgttgctc acttgcggac gatatcgcgt agaacctaag aagcgtcggg cgcaagcagc
      121 gtcaatttcg gaaaataagt acgatagcgc cggcaatcgc gcgtcagcaa aggcgcgccg
      181 agcgatagag cgtgcgcacc gatgaagaaa tcagggagga cattcgtgcg ggcgccgccg
      241 gccaatctat attccttgaa ggcttgcgcg gcgccgaaag ccgccgcctt cgggatgtcg
      301 gcgatggtca gttggaaaga ttccagttcg gcatcgcatt ctgcggcggt cgcaaaattg
      361 attgcaaatc ccgcatagac gacgacattg atgacgaggg ggccacgctc atgagcggcc
      421 gcgagcgccc gacgcgacca gtcgccccag cgcgaattgt tggacgcaat gtcgatcaga
      481 atattggtat cgacgaacgt caatcttcgt tccgagtcat cgccatgact tcttcagtcg
      541 tcagatttcg gatcggcttt cgccggctga tcgcttcgag ccgctggaga tacgcgtgcg
      601 gaccagcatg gttggcggcc gactcgacaa tcacccctcc ctccggccta acgcgaacag
      661 tgacgcggtc gccggggcga atgccggcag cctcgcgtac tgcctttgga agggtgactt
      721 gcccttttac ggtcactgtt gtggtcatct gtggttccgc ctgtgcgacg ctattaaaac
      781 gcgagtcaga tcggccgttc aggatgagat cgagaaggac attcgtgtcg acgaacgtaa
      841 cgaacgcagc ccagcgatgg cgcgatcgat ccgctttata ttctccgcaa ccgcttgcgg
      901 atccggctcc aacgtttcgc gctcgacaat caggcctcct ttcggccggc gcgtacgacg
      961 accctgtcgc cgggcctgaa gccggctgct tcgcgcgccg ctttcggcag ggtgacctgc
     1021 cctttgacag tgactatcgt agccatgacg tatcgtaata ctcttcacta taagggtatt
     1081 accggacgcc tgcggcgtca aacaattgcg catgcggcgc tccggactag ccttcactga
     1141 aagcgtcaca aaccaatcaa aatgtcgcgc catcaagcat ttatcacaga gcccgcgcgc
     1201 cgagtcgcgg tgatccatga gaccgacgtg ctggtcgtcg gctccggccc gggcgggctg
     1261 gcggcggctt tggccgccgc gcgcgccggc gtcgaggtcg cgctgatcga gcgcttcggc
     1321 tgtttcggcg gcaacatcac gacggtcggc gtcgagggct tcgcctggta ccggcacgca
     1381 gcgacaatcg aggccggcgg aatcggccgc gaattcgaag agcgcgccaa ggcgatgggc
     1441 gcggcggtcc cggaaagcca gtcgctgagc tacgagctcg attccgaagg cttcaagctg
     1501 gtcgccgatc gcctcgtcga ggaggcgggc gtccggccaa tgctgcacag gctcttcgtc
     1561 gcgccgatcg tggatggcga cgcggtcaca ggaatcatcg ccgaatccaa ggccggccgc
     1621 gaggcgatcc tcgccaagcg ggtgatcgac gcgaccggcg acgccgatat cgcccatcgc
     1681 gccggcgcgc cgaccaacaa gacgccggtc gaagagatgc aggccgcctc ggtgatgttc
     1741 catctcgccg gaatcgacaa gcgggcgttt ctcgaaggcg tgcgcgccga tccgcagacc
     1801 tatggcgact ggtcgaccgg cgagtggacg gtcgagacct ccggcaagga ggacaagatg
     1861 ttctcgccgt ttctggccaa gcctttcgcc gaggcgatca aggccgggct catcccagcg
     1921 cggctcaaca cgatcggcgg cacttgggga gccgtccatg ataccggcga gctcacctat
     1981 atgaatttga tccatctcgc cggctgcgac gggaccgatc ccgacagcct gacgcgcttc
     2041 gagatcgagg gccggcgaca ggcgatgctg gcgatcgaag ccttgcgccg ctttacgcct
     2101 ggctgcgccg gcgcgcggct gcgcaatttc ggcatgacga tcggaatcag ggacacgcgc
     2161 aagatagacg ccctctacaa tctgaccgaa caggacgtgc gcaacgaggc gcggttcgag
     2221 gattcgatcg gcgtctatcc cgaattcatc gacggctatg gcgtgctgat cctgccgacg
     2281 accggacgct acatgcatat cccttaccgc gcgctgctgc cgaagcaggt cgagaacctg
     2341 ctggtcgccg gccgcgccat cggcggcgac cgcatcgccc atgccgcgac ccgcaacatg
     2401 gcctgctgcg ccgtggcggg acagggggcg ggaatcgccg cagccttgtc cgtcaagact
     2461 ggccgaagcc cgcgcgaggt cagcctcgcc gcgattcatc gcgagctcga ccggcaaggc
     2521 gtacgattcc aatagggcgc ggagctttgc gggtttgccg gtgaatctgt acctcaatga
     2581 cgccgtgacg ggtgccgcct atcctcgata cagcgcatgg ctgtggcgcc agaacactcg
     2641 gcgtcactgc gcggcgaagg tatctacaag gctttgcgct tgctgggtca gagcgggaaa
     2701 agctgcggtc gcgccggctt tttcggacca gcatttgtgg aacgccgccg ccccttcgtt
     2761 ttccagccgc gtcgattcgt cggcgctctt cgggtcgtcc ttccccgctt gctggtacgc
     2821 cttggccgcc gcttcgctcc tggcttggaa gtctttccag atggcggcgc aggccgcaat
     2881 cggcgcgggc ttggccgcgg ccttgacgat tgcgacatag actctgccgc ccttggcgac
     2941 ggcgaccgcg atctgttcgg gcgggtcgac ggcgacgtcc tgcgcttgct ggataagcag
     3001 cgccgccgcg gtcgccgcgc cctctggctt tcggatcggc agcatcgcga agatcgagac
     3061 ggccgcatcg gggctggccg cctgggtgta gaacgcctcg gagtggaaag cggcctcggg
     3121 ctcgaccggc gggttgggca gatctttcca ataattcctg tgcgctttga gccaggcttt
     3181 caggagcccg tcggtcgtga ccagcacggt gttctcgtcc ccgttgcgaa agacgaggcc
     3241 gtccagcccg tcggcgccga tatcggtctg gatcagactt tccggactga ttgtcccggg
     3301 gccagggaaa tccttgagcg gcggcggatt gatcacgctc ttcaattgcg cctccaagcc
     3361 ggcgcgcaac cggcgctcct cggcgtcctg cttatcgccc ttcatgtgct tgagcgtggc
     3421 gacggcgtga tcgcgggcgg cgagataggc ttgctcggac ccggaggcga aagcggggga
     3481 ggcggcggcc agtaaggcga gcacgaccag gttgcgcaaa tccgcccctc cttctatagc
     3541 cggccggcat gttcacgctt cccgcgccga acgagggcgc ggccgataat cgcgaggccg
     3601 aatctggcga acacgacgtc ctagcctaaa gaccgggagc gcgcacgcga agagcggcgt
     3661 ctccagattc ggacagcgcc gagcggccga acgtccatcc aaagacaacc gacgcgcttc
     3721 gtcggggact gatcgcgcag cagcgcggcc acgatcgtcg atagcgcttc gccggaaatc
     3781 tccatgaatg aatcgatatc cgcccgcgcc tctggctggc cggtctgggc gccgatcgcc
     3841 gcgatgttct tcgtcaatgg cgcggtctat ggcgtgtggg cgacgcaaat tccgctggcc
     3901 aagctgcgcc tgggcctcga tccttcgcaa ctcggcgcga cgctgttcgt tctcgggctt
     3961 ggcgccatcg cggcgatggc ggcgagcggc tggatcctgc aacggatcgg cgcggcggct
     4021 ttgatccgca tcagcggcgg cgtgttttgc attctgctgc cccttgtcgg cagcgcgccg
     4081 agcgctcggg cgctggccgg gcttctcttc ttcttcggcg cgagcggcgg cttgatggac
     4141 gtcgccatga acgttgccgc cgcagaagcc gaacggcgcg ccaagcggcc ctatatgtcg
     4201 tcgttccacg gcatgtggag cctggccggg ctgaccggcg ccggcggcgg cacgttgctc
     4261 ttgaacgtcg tcagcggccc ctggcaaagc ttcgtcatgg ccgcgcttct cgccgcggtg
     4321 ttcgtacttg gccaggcgcg gctgcgcggc gagccggagc agcgcgccga ggcggcggcg
     4381 cggcggccgg cgctgcgccc gaccctcgcc gcgctcctcg tcggcctgat ggcggctttg
     4441 tgcttcagcg gcgagggcgc ggtgctcgat tgggccgccg tctatctgcg cgacagtctg
     4501 ggcgcggcga tcgagcgcgc caatctcggc tatgccgcct tttccggcgc gatggcgttc
     4561 ggcaggttct tcggcgaccg catccggcgc agcgtcgacg gcccggcttt ggtgcgcggc
     4621 gggtgcgtcc tggtgtttat cggcttgctg gcgggcccgc tctcgaacaa tttctggatc
     4681 gccgtcgccg gctacctttt gaccgggttg ggcttctcca acatggtgcc ggttctgttc
     4741 agcgcggccg gcgccatgcc gcatccggaa acacagatcg ccgccgtctc cactttgggc
     4801 tatgccggac tgctcgccgc gccgccgctc ttcggcttcg tcgcgcaggc gacctcgctc
     4861 gccggcattt tctacctggt ggccgcggcg accatcctga tcgcggcgct ggcgggcgtt
     4921 tgcgcgccgg cgcggcgcgt gtcgccgcaa tcctgattcg ccgcgcattc acgcgccgcc
     4981 gcgaagggcg cacgccgatc tgttgagcgg ccgggtcgta tgctcggcga tttgccgctg
     5041 tttggtccgc gcgctaaagc cgtcccatgc ccgcgcccgc cgaccgcctc gtgatcgccc
     5101 tcggacagct cgacgccaca attggcgata tcgagggcaa cgcccagcgg ctccggcaag
     5161 cgcgggccga agcggccgct ttcggcgccg acattctcat gtcgccggag ctctttctct
     5221 ccggctaccc gccggaagac ctggcgctca agccggcgtt ccaagaggct tgccgcgcgg
     5281 cttgcgaggc cttggcgcgc gagaccgccg atggcggccc ggctctgctc gtcggcttgc
     5341 cctgggccga aaacggcgcg ctgtacaatg cttacgccct gctcgacggc ggccgcatcg
     5401 aagccgtgcg cttcaaggtc gatctgccga attacggggt gttcgacgag aagcgcgtct
     5461 ttaagcccgg ccccgcgccg gggccgatcg tgtttcgcgg cgtcaggatc ggcgtgccga
     5521 tctgcgagga catttggggg ccggagccgg tcgaatgcat cgccgagacg ggcggcgaaa
     5581 tcctgctcgt gcccaacgcc tcgccttacg agcgcgacaa ggtcggaacc cggctcaaca
     5641 tcgcggtcaa ccgcgtcgtc gagagcggcc tgccgctgat ctatctcaac caggtcggcg
     5701 gccaagacga gctggtcttc gagggggctt ctttcgcgct caacgccgac cgctcgctcg
     5761 cctgccaaat gccggctttc cgccaactcg tcgcgcgcac cgtctgggag cggcaaggcg
     5821 agacctggtg ttgcgtcgag gcgccgcgcg agatcgtcga agagggcgac gaagccgatt
     5881 acgccgcctg cgtgctcggc cttcgcgact atgtcgagaa gaaccgcttt cccggaatcg
     5941 cgctcggcct ttcgggcggc gtcgattcgg cgctgtgcgc ggcgatggcg gtcgacgccg
     6001 tcggcgccgc ccgcgtccac gcgatcatgc tgccctaccg cttcacgtcg ggcgaatcgc
     6061 tcaatgacgc ggcggcttgc gctaaggcgc tgaacatccg ctacgacacg atcccgatcg
     6121 ccggtccggt cgaaacgatc gagaaggctt tgcagccgct ctttgccggg cgcatccgcg
     6181 acgtcacgga agagaatatc cagagccgcg cacgcggcct gatcctcatg tccgtctcca
     6241 ataagcttgg cgcgatgctg gcgacgaccg gcaacaagtc ggaactgtcg gtcggctacg
     6301 cgacgctcta tggcgacatg aatggcggct tcaacccgct gaaggacctc tataagacgc
     6361 aggtcttccg cctcgcccaa ttgcgcaacc gctggaagcc gttcggcgcg ctcgggccgg
     6421 acggcgaagt catcccgacg aatatcctga ccaaggcgcc gagcgcagag ctgcgcgaga
     6481 atcaaaagga tgaagattcg ttgccgccct acgcggttct cgacgcgatc ctggagggtc
     6541 tcgtcgagag ggagatgcgc gtcgccgaca tcgtcgcgca aggctacgac gccgagacgg
     6601 tcaagaaagt tgagcgcctg ctctatctcg ccgaatacaa gcgtcgccaa tcggcgcccg
     6661 gcgtgaaagt cacgtcgaag agcttcggcc gcgaccggcg ctatccgatt gtcaatcgct
     6721 tccgcgattc aggcgagccc aagcgcgagc cggacgcggc gatcgcgccg gcgcggccga
     6781 ttgggacgag cgaaaggttt gaggactagg ttaatccgag tacgttctgg atatccgcca
     6841 tggtcgccat caacatcatc ggttcaagcg gcgatggatc gaaaccaagc gacaagtaga
     6901 aagctttcgc ctcatccgag atcgcatgga cgagcacgcc tcggatgcca agggtctccg
     6961 cggcgctggc gagccgcatc gccgcgtcgc gaaagagcgc tcgtccaatc cccttgcttt
     7021 gccagcgatg atcgaccgca agcctggcga gcaaggccac tggaatcgga tccggcatgt
     7081 tgcgacgaaa gcggccagtg gcagcggcaa ggctgacgcc gcccgcagcg agcgcgtaat
     7141 agccggcgac cacattatcg tcgcaggcga cgaacgttcg cgtcgcgccg ctgatctgat
     7201 tggcgcgggc gcgtcgtttc aaccaatcgt cgagcgaggc gtttccgcag tcgaagcgcg
     7261 taagatcgtg ccgattggcg agcggcgcgg gcgccgccag cgtcacgacg catcccaggg
     7321 cgctttcgtc gtcagcgcgc ggcggagacg gtcgttcggc cgcggcggtg cgtcgagccg
     7381 ctccataaat ttggcgtagg ctgctggatt tgctgaaaat accgcccgat cgagcagcgc
     7441 ctcttcagcc gctcgcttcg cggcttcgag cataaaatcc gtgcgcgtct tgccgagcgc
     7501 cgcggcggcg cggtcgatca ggccgcgctc ctcggctctg atgcgaatgt tgagcgtgtc
     7561 gcgggcaata ctgcgcttgc cggctgggcg ctgtgcgccg cttcgatcgg atgtcttttt
     7621 tgcggcgggc cgcggcatgg aagttcctcc gacaattttg agtctagcac tgtgtatagg
     7681 cgtgtcatca caaaacaatc cgacgccgcg acctcaccca ttctccctca gatagttccg
     7741 cggcgaagcg cccagcaccc gcttgaacat cgtcgtgaac gccgccggat tgtcgtagcc
     7801 gagatcgagc gcgacctccg tcaccttttc gcccgccgtc aggcgcggca gcgcggcgac
     7861 gaggcaggcc cgctgacgcc acagcgcgaa gctcaggccc gtctcgcgct tgatcagccg
     7921 ggtgaaggtt cgccggctga cgccgagcgc ctcgctccag gcgtcgatcg tcgcatgcgg
     7981 gctcggcttt tcgaggaagg cgcggcagcg ctcggcgagc gcgccatggg tcgggaaggg
     8041 cagcgacagc ggcagcgccg gcaggcgcct gatctcgtgc aggaccagcg ccatcagcgc
     8101 gccgccgcgg ccgccgaggt cgtaatcggc cggcagatcg acggcctcgg ccaagagact
     8161 gcgcatgaag ggcgaaatcg ccacaacctg gcaatgatcg ggcatgcctt gggcgacctc
     8221 aggttcgaga tagaggctgc gcgcgctgaa cgcgccgagc atgcgcacct cgtgcgtcac
     8281 gccggccgga atccacatgc cgccctgcgg cggcatcacc caggcgctct gctcggcggc
     8341 gaaaatcacc gcgccggacg cgccataaag caattggctg cggcgatgac ggtgcggcag
     8401 gacgacgtgg ccgctcggat agtcattgcc gatggcgatg acgggacgcg gcacgtgatc
     8461 gaaagcgtca atgcgggcgt ttcggaccat ggcccaaact cataagacgg cggcctgatt
     8521 gcggtggcgg gcgccgcgct cccgccatag cctcccaggt cctgcgattc aaccgcgaat
     8581 ggccgggccg acgcgtcggc cggcagagag gcgctttcgt gagtcaagcc gtcatcgccc
     8641 agtccaagac cggcgcgacc gcattttcga ttcttggagc gatcagcttc tgtcacctgc
     8701 tcaacgacat gatgcaggcg ctgctgccgg cgatctatcc aatcctcaag ggcggcttcg
     8761 acctctcctt cggccagatc gggctgctga cgctgaccta tcagatcacg gcttcgctgc
     8821 tgcagccgct ggtcggtctc tataccgatc gccggccgca gccctattcg ctgcccttcg
     8881 gcatgggctc gaccttgctt ggcctcgtcg ccctggcctt cgcgcctaat tacctgacgc
     8941 ttgtcgccgg gtcggcgctg ctcggcgtcg gctcgtcgat ctttcatccg gaatcttcgc
     9001 ggatcgcccg actcgcctcc ggcggggcgc atggcttagc tcagtcgctg ttccaggtcg
     9061 gcggcaattt cggtcaatcg ctcggaccgc tcctggcggc tttcgtcatc ctgccgcacg
     9121 gacgcgccag cctcgcctgg ttcgcgctcg ccgcgctcgg cggcatggcc attctcgcgg
     9181 cgctcggcaa ttggtcgaag cgcaagggcg cggcgcggcg gtcggcgcat aaactcgcgc
     9241 ccgagcgaag cgccttgcct aaagcacggg tggcgcgtgc ggtggcggtg ttgatcgcgc
     9301 tgatcttctc taagtatttc tatctcgcga gcatcaccag ctactacatt ttctacctga
     9361 tgcggcgctt cgatctgccg acgcagacgg ctcaggtcta
//
LOCUS       DATGJW010000524         9371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_510797, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000524 DATGJW010000000
VERSION     DATGJW010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 9371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9371
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_510797"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..474
                     /gene="queF"
                     /locus_tag="VKU03_15900"
     CDS             61..474
                     /gene="queF"
                     /locus_tag="VKU03_15900"
                     /EC_number="1.7.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002711647.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033739 - preQ1 synthase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preQ(1) synthase"
                     /protein_id="HLJ72800.1"
                     /translation="MPNEPAAAILERVPNPHADADFVTRFTAPEFTSLCPVTGQPDFA
                     HLVIDFVPDRWLVESKSLKLYLASFRNHGAFHEDCTVRIGKDIAGLLKPRWLRIGGYW
                     FPRGGIPIDVFWQTGALPKGVWAPDQGVAPYRGRG"
     gene            497..1357
                     /gene="sseA"
                     /locus_tag="VKU03_15905"
     CDS             497..1357
                     /gene="sseA"
                     /locus_tag="VKU03_15905"
                     /EC_number="2.8.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020178325.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004792 - thiosulfate sulfurtransferase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-mercaptopyruvate sulfurtransferase"
                     /protein_id="HLJ72801.1"
                     /translation="MGSHNFVSTEWLAGRLNAPELGVIDASWHLPTAGRNGAEEFRQG
                     HIPGAVFFDIDTIADLSSGLPHMLPDEAAFAAAMGKLGLDENMTLVVYDSLGLFAAAR
                     VWWTLRAFGAGKVKILEGGLPKWIREGRPLEQGDAARPPRTFHARLDPRAVASRADVG
                     RALAGGAVQVVDARSADRFEGRAPEPRPGVKSGHMPGAFNLPFVEIVDAGKLKSPPAL
                     AETFAAHGLDLKRPMITTCGSGVTAAILALAAEEAGGKVVGLYDGSWAEWGSRDDCPI
                     ETGPGRVAAP"
     gene            2068..5196
                     /locus_tag="VKU03_15910"
     CDS             2068..5196
                     /locus_tag="VKU03_15910"
                     /inference="COORDINATES: protein motif:HMM:NF012789.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HLJ72802.1"
                     /translation="MRQAFHADLHIHSKYSRATSRDLDLERLAFWAARKGVAVVGTGD
                     CVHPAWLAELKDKLVPDGRGLFRLKDQIEREIFKTLPPACRASVRFMLSTEISTIYKK
                     GEKTRKIHHLIYAPDFDSADRLASSLARIGNIASDGRPILGLDSRDLLEIALQSGPDS
                     YLVPAHIWTPWFAALGSQSGFDSIEECYGDLAGHIFAVETGLSSDPAMNWRISSLDRF
                     RLTSNSDAHSPGKLGREATRFCCEPDYFAIRRALETGEGYVGTVEFFPEEGKYHLDGH
                     RACGVRLEPTETLALGGRCPACGGKVTIGVAHRVETLADRSESEAAPPPTAGAVTSLT
                     PLPEILSELLESGVASQSVTRAYDRVTATLGPELSILQEAPIEDVAQADPLLGEAVTR
                     LRNGAVIREAGYDGEYGIIRLFEEAELRRRTKGGLLFDAPAPRRRMASAAAVKPRKPA
                     PAEAAPAADPTPSSTSLGVLAGLDEDQRRAAAATDGAVIVLAGPGAGKTRVLTRRLAH
                     LVLECGASAAACLAVTFTRRAAAELQERLAALIPAEAGQCAVHSFHALGLAILRADGG
                     AIGLRPDFQVAEERRRAGALAREMQIPEAKAARLLKALSFSKRTGGMLTGADAEALAA
                     CRRIDAANNWIDFDDLIGLSTQLLTSDAGVAARWRERFRNICIDEFQDIDEQQYKLIR
                     LLVGDHANLCAIGDPNQAIYGFRGADASCFERLAGDFPGASIFRLARNYRSSGAIVQA
                     SAQAIGASAAAGVMRPLGAPIAVHIAADERAEVEYVAATIESLLGGHDLLAAVRHGAG
                     GAARPLSFADFAVLYRTDAQAAILREAFDEAGIPFKKSSPAAITGQPAVRAVLRALDR
                     QEDHSVPLMKRIDAAAEQVGAEGKEFDAADLAEARRWLAALACGDDEARLREQVALSN
                     EADFYDRRAERVSLLTMHAAKGLEFAVVFVVGLEDGLTPFSWSASRGDAREDDDGRDS
                     AAEERRLFYVAMTRAKERLFLTRAAQRFWRGQIRALPPSPYLAAIAADLIASDLLPRR
                     TKRTAPKQYSLF"
     gene            complement(5202..6035)
                     /locus_tag="VKU03_15915"
     CDS             complement(5202..6035)
                     /locus_tag="VKU03_15915"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ72803.1"
                     /translation="MKAAAKLAPAAKLANGLEMPRIGLGVYLVPSGRKTRDAVASALK
                     LGYRHIDTARLYDNEADVGQAMRDSGVPRGELFVTTKLWNADHGFDRTLAAFDESRAA
                     LGLEAVDLFLLHWPVPRLRAESWRALERLYDEGRVRAIGVSNFMIHHLEELMAGARIA
                     PMVNQIEVHPFFQQRDVRAWCAKHGIAVAAYSPLAKGAVARDPAVRKVAAEAGVTPAQ
                     AALAWALQHDMIVLPKSVRPERQAENLAAAQVKLSAAQIAALDGLDRGHVTDWDPRAA
                     P"
     gene            complement(6032..6784)
                     /locus_tag="VKU03_15920"
     CDS             complement(6032..6784)
                     /locus_tag="VKU03_15920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009799623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF502 domain-containing protein"
                     /protein_id="HLJ72804.1"
                     /translation="MTLAPNKISETAAAASARSLGARVRTWFFTGLVIFGPVAVTLYI
                     AWWAIDTVDNWVKPLVPARYWPDSYLPVHVPGFGVVIAFIGLTLLGFLTANIAGRTLV
                     RIGELILQRMPFVRGVYKSVKQIFETVFSQGGSHFRRVGLVEFPQGCWSLVFISAEPS
                     QVIAGGLPAGDHLSVFLPCTPNPTTGFYFFVPAEGVIELPMTPEDAAKLIMSAGLIQP
                     EGQAALAAMAKTAKEARAAAPQAALAGGASGA"
     gene            7064..7969
                     /locus_tag="VKU03_15925"
     CDS             7064..7969
                     /locus_tag="VKU03_15925"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009764138.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2189 domain-containing protein"
                     /protein_id="HLJ72805.1"
                     /translation="MANFHVYLGQDVRAGLPVIRKVAPSDIRDALIKGFDDFRAMPSH
                     LLFLALIYPLCGIVLAYVTSQQNLLQLLFPLASGFALVGPFAAIGLYEMSRRRELGLD
                     TSWRHAFEVIRSPSIPSIIALGLALVAIFALWLAAAQVLYSFLYGPIPPSSYGAFLGD
                     IFSSGRGWALIVIGCFIGFCFAVIAFSISVVAFPLLLDRDVGLAAAIGASVKCVLDNP
                     VTMALWGLTIAALLAIGSLPLFLGLVVIMPVLGHATWHMYRKAVERDPAHEHLADWPA
                     DRMGRPASQVASAHAVLFPWPKSEG"
     gene            complement(8675..9014)
                     /locus_tag="VKU03_15930"
                     /pseudo
     CDS             complement(8675..9014)
                     /locus_tag="VKU03_15930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335690.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
     gene            complement(9016..9318)
                     /locus_tag="VKU03_15935"
     CDS             complement(9016..9318)
                     /locus_tag="VKU03_15935"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010335689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1778 domain-containing protein"
                     /protein_id="HLJ72806.1"
                     /translation="MPKTAARKEHPLSMRLPEADIALIDRAATLRGRSRTDFVRDAAV
                     RAAEDVLMETRPIRMSPADFKAFMRALSEPAAPAPEMVELFHRAAPWEAGGSGGGR"
BASE COUNT         1564 a         3183 c         3008 g         1616 t
ORIGIN      
        1 cccaaagcgt catcccaacc gccgctcagc ggtctcacgc atctcggccg ctcctcgacg
       61 atcccgaacg agccggccgc cgccattctc gaacgcgtcc ccaacccgca tgccgacgcc
      121 gatttcgtca cgcgcttcac ggcgccggaa ttcacctcgc tttgtccggt gaccggccag
      181 cccgatttcg cccatctcgt catcgatttc gtccccgatc gctggctcgt cgaatcgaag
      241 tccttgaagc tctatctcgc cagctttcgc aatcacggcg cgtttcatga agattgcacg
      301 gtgcggatcg gcaaggacat tgcggggctg ttgaagccgc gctggctgcg catcggcggc
      361 tattggttcc cgcgcggcgg catcccgatc gacgtgttct ggcagaccgg cgccctgccg
      421 aaaggcgtct gggcgccgga ccagggcgtt gcgccctatc gagggcgcgg ctagagcgcg
      481 gaaggacgga cctcttatgg gctcgcacaa tttcgtttcg accgaatggc tcgcgggccg
      541 gctcaacgcg cctgagcttg gcgtgatcga cgcgtcgtgg cacctgccga cggccggccg
      601 caacggcgcg gaggaatttc gccaaggaca tattcccggc gccgtctttt tcgacatcga
      661 tacgatcgcc gatctgtcca gcggcctgcc tcacatgctg ccggacgagg ccgctttcgc
      721 cgccgcaatg ggcaagctcg gcctcgacga aaacatgacc ctggtcgtct acgactcgct
      781 cggccttttc gccgccgcgc gcgtctggtg gaccctgcgc gccttcggcg caggcaaggt
      841 gaaaattctc gaaggcgggc tgccgaaatg gatcagggag ggccggccgc tcgagcaagg
      901 cgatgcggcg cgtccgccgc ggactttcca cgctcggctc gatccccgcg ccgtcgcttc
      961 gcgcgccgat gtcggccggg ctctggccgg cggcgcggtt caggtcgtcg acgcgaggtc
     1021 ggcggatcgt ttcgagggcc gtgcgcccga gccgcggcct ggcgtcaaaa gcggccatat
     1081 gccgggcgcg ttcaatctgc ctttcgtcga aatcgtcgat gcgggcaagc tcaaatcgcc
     1141 gcccgcgctc gccgagacct tcgcggcgca tggcctcgac ttgaagaggc cgatgatcac
     1201 cacctgcggc tccggcgtca ccgccgcgat cctggcgctt gccgccgaag aagccggcgg
     1261 taaagtcgtc ggcctctatg acgggtcatg ggcggaatgg ggcagccgcg acgattgccc
     1321 catcgaaacc gggcccggac gggtcgcggc gccataaaat cgcggcgcgt acggcatcgt
     1381 acccaattga tcgatggcga agcgaattcc gcttggctgc ggagatgcgc ggcgaggaaa
     1441 aagcctttcg aaggtagaat gcgtttggac gtcgtcgcgg cgattcccgg cgcgccgaag
     1501 ccggcgccct gaaacgccgg gccgctcgcc gagcgatgtt taaaccgcgt tgaaagagga
     1561 acgatcgcgc ccaagcctcg ttgaccgctg cgagcgagcg ggcgtcatac gagatttcgt
     1621 tccggatgct tgccgctccg gccggccccg ctgtctccgt cggggtcggc cgcgcttttt
     1681 ccgcgacacc gccgcgccaa aaaacacgaa acgcggaacc gcgcgccgcc aaggcaagtt
     1741 gggccggcgg caggagcctt tcagcaagaa attcgcggac cctgacgaga ccaatgaccc
     1801 ggccgaagct gcttgccgcc gtcgcggcga tcagcccctc gttggccggc gcgccgccag
     1861 cagcatatcg accaaatcgc cggccagcat ggctgacgcc gcttcgccgt gaacgacgag
     1921 cgccgccgaa ggcggcgcct cctgggcagt cccgatccgg ctccgcgctt actccgcttg
     1981 caccgggcga gcgcttccag tggatgagtc gggcttggcg cggtcgcgcg gtttgaaccg
     2041 cgcgcgccgg ccgtctaacc ctatcggatg cgccaagcct tccacgccga ccttcacatc
     2101 cattccaaat attcgcgcgc caccagtcgc gacctcgatc tggagcgcct cgccttttgg
     2161 gccgcgcgca aaggcgtcgc cgtcgtcggc acgggcgatt gcgttcatcc agcctggctc
     2221 gccgagctca aggacaagct cgtccccgac ggacgcggcc tctttcggct gaaagaccag
     2281 atcgagcggg agattttcaa gacgctcccg cccgcgtgcc gggccagcgt gcgcttcatg
     2341 ctctcgaccg agatctcgac catatacaag aagggcgaga agacgcgcaa aatccatcac
     2401 ctgatctatg cgccggattt cgacagcgcc gaccggctcg cttcgagcct cgcccggatc
     2461 ggcaatatcg cctcggacgg acggccgatt ctcggcctcg actcgcgcga ccttctggaa
     2521 atcgcgctgc aatccggtcc ggattcgtac cttgttccgg cccatatctg gacgccctgg
     2581 ttcgcggcgc ttggctcgca atcgggcttc gactcgatcg aggaatgcta cggcgatctc
     2641 gcgggccata ttttcgcggt tgagaccggc ctctcttccg atcccgcgat gaattggcgc
     2701 atttcgtcgc tcgatcgctt ccgcttgacc tccaattccg acgcccattc gccgggcaaa
     2761 ctcggccgcg aggcgacgcg cttttgctgc gagccggatt atttcgccat ccgccgcgcc
     2821 ttggagaccg gcgaaggcta tgtcgggacg gtcgagtttt tccccgaaga gggaaagtac
     2881 catctcgacg gccatcgcgc ctgcggcgtc aggctggagc cgaccgaaac gctcgccttg
     2941 ggcggccgct gcccggcttg cggcggcaaa gtcacgatcg gcgtcgccca ccgcgtcgaa
     3001 accctggccg accgcagcga gagcgaggcc gcgccgccgc cgacggccgg ggccgtgacc
     3061 agcctgacgc ccctgcccga aatcctctct gaacttttgg agagcggcgt cgcctcgcag
     3121 agcgtgacgc gcgcctatga ccgcgtcacc gcgacgctcg gcccggagct gagcattctt
     3181 caagaagcgc cgatcgagga tgtcgcgcag gccgatccgc tgctcggcga ggcggtgacg
     3241 cgcttgcgca acggcgcggt gatccgcgaa gccggctatg acggcgaata cgggatcatc
     3301 cgtctgttcg aggaagccga gctgcgccgg cgcaccaagg gcggcctgct gttcgatgcg
     3361 ccggcgccgc ggcggcgcat ggcgtcggcg gcggccgtca agccgcgcaa gccggcgccc
     3421 gccgaagcgg cgcctgcggc cgaccccacg ccgtcttcga cgagcctggg cgttttggcc
     3481 ggtctcgacg aggatcagcg ccgcgcggcc gcggcgaccg acggcgcggt catcgtcctc
     3541 gccgggccgg gcgccggcaa gacgcgcgtg ctgacgcgaa ggctcgcgca tctggttctc
     3601 gaatgcggcg cttccgccgc ggcctgcctc gccgtcacct tcacccggcg cgccgccgct
     3661 gagctccaag agcggctcgc cgcgctcatc ccggccgagg ccggccaatg cgccgtgcac
     3721 agctttcacg cgctcggcct ggcgattctc cgcgccgatg gcggcgcgat cggccttcgt
     3781 cccgatttcc aggtcgccga ggagcgccgg cgcgccggcg ccttggcgcg cgagatgcaa
     3841 attccggaag ccaaggcggc gcgtctgtta aaagcgctgt ccttctcgaa acgtaccggc
     3901 ggaatgctga ccggcgcgga cgccgaagcg ctcgccgcct gccggcgcat cgatgcggcg
     3961 aataactgga tcgatttcga cgatcttatc gggctttcga cgcagctcct aacgagcgac
     4021 gcgggcgtcg cagcgcgttg gcgggagcgt tttcgcaata tctgtattga cgagttccag
     4081 gacatcgacg agcaacagta caagttgatt cgtctcctcg tcggcgacca cgccaatctc
     4141 tgcgcgatcg gcgatccgaa tcaggcgatc tatggctttc gcggcgccga cgcttcgtgc
     4201 ttcgagcgcc tggcggggga tttccccggc gcgtccatct ttcgcttggc gcgcaactac
     4261 cgttcgagcg gcgcgatcgt ccaagcgagc gcccaggcga tcggcgcgag cgccgccgcc
     4321 ggcgtcatgc ggccgcttgg agcgccgatt gccgtccaca tcgccgccga cgaaagagcc
     4381 gaggtggaat atgtcgcggc gacgatcgaa agcctgctcg gcggccacga tcttctcgcc
     4441 gccgttcgcc acggcgcggg cggcgcggcg cggccgttga gtttcgccga tttcgcggtt
     4501 ctctatcgta ctgatgcgca agcggcgatt ttgcgcgaag cgttcgatga agccggcatc
     4561 ccgttcaaga aaagctcgcc cgcggcgatc acgggtcaac cggcggtgcg cgccgtgttg
     4621 cgcgcgctcg accggcagga ggaccattcc gtaccgctga tgaagcggat cgacgcggcg
     4681 gccgaacaag tcggcgccga agggaaggaa ttcgacgcgg ccgatttggc ggaagccagg
     4741 cggtggctcg ccgccctcgc ctgcggcgac gacgaggcgc gattgcgcga gcaggtcgcg
     4801 ctgtcgaacg aggccgactt ctacgatcgc cgcgccgagc gcgtctcgct cctcaccatg
     4861 cacgcggcca agggtctgga attcgcggtg gtgtttgtcg tcgggcttga agacgggctg
     4921 acgccgttct cgtggagcgc gtcccgcggc gacgcgcgtg aggacgatga cggtcgggac
     4981 tcggcggccg aagagcgccg tctcttctat gtcgcgatga cccgggcgaa ggagcggctg
     5041 ttcctcaccc gcgcggcgca gcgtttctgg cgcggacaga tccgcgcctt gccgccgtcg
     5101 ccctatctcg ccgcgatcgc ggcggacctg atcgcgtccg atcttttgcc gcgtcggacg
     5161 aagcggaccg cgccgaaaca gtacagccta ttttgagcgc gtcacggcgc tgcgcgcggg
     5221 tcccaatcgg tgacgtggcc gcgatcgagt ccgtctagcg cggcgatctg cgccgcgctg
     5281 agcttgacct gcgcagcggc gagattctcc gcctgacgct ccggccgcac cgatttcggc
     5341 aggacgatca tatcgtgctg aagggcccag gcgagcgccg cctgggccgg cgtcacgccc
     5401 gcctccgccg ccacttttcg caccgccgga tcgcgcgcca ccgcgccctt ggccagcggc
     5461 gaataggccg caaccgcgat cccgtgcttg gcgcaccagg ccctgacgtc gcgctgctga
     5521 aagaacggat ggacctcgat ctggttgacc atcggcgcaa tccgcgcgcc ggccattaac
     5581 tcttcgagat gatggatcat gaaattgctg acgccgatcg cgcgcacgcg gccctcgtca
     5641 taaagccgct ccagcgcgcg ccaggattcc gcccgcagcc gcggcacggg ccaatgcagg
     5701 agaaagagat cgaccgcttc gaggccgagc gccgcgcggc tctcgtcgaa cgcggcaagg
     5761 gtacggtcga agccgtgatc ggcgttccac agcttggtcg tgacgaagag ctctccgcgc
     5821 ggaacgccgc tgtcgcgcat cgcctggccg acatcggcct cattgtcgta aagtcgcgcc
     5881 gtatcgatat ggcgataacc gagcttcagc gccgaggcga ccgcgtcccg cgtcttgcgg
     5941 ccggagggca cgagatagac gccgagcccg atgcgcggca tctccaagcc attggcgagc
     6001 ttcgccgccg gggcaagctt ggcggcggct ttcatgcgcc gctcgcccca ccggcgagcg
     6061 cggcttgcgg cgcagcggcg cgggcctctt tggcggtttt cgccatcgcg gcgagcgccg
     6121 cctgcccttc cggctggatg aggccggccg acatgatcag cttggccgcg tcttccgggg
     6181 tcatcggcag ctcgatcacc ccctcggccg gcacgaagaa atagaagccg gtggtcgggt
     6241 tgggcgtgca gggcagaaag acggaaaggt gatcgccggc cggcagcccg ccggcgatga
     6301 cctgcgaggg ctcggcggaa atgaacacga gcgaccagca gccctgcgga aattcgacaa
     6361 gcccgacccg gcggaaatgg ctcccgcctt gcgaaaacac cgtctcgaaa atctgcttga
     6421 cgctcttata gacaccgcgc acgaagggca tgcgttggag gatcaattcg ccgatccgca
     6481 cgagcgttcg cccggcaatg ttcgcggtca aaaagccaag gagggtcagg ccgataaagg
     6541 cgatgacgac gccgaagcca ggcacatgga ccggcagata ggaatcaggc caatagcgcg
     6601 ccggaacgag cggcttgacc caattgtcga ccgtgtcgat cgcccaccag gcgatataga
     6661 gcgtcaccgc gaccgggccg aaaatgacga ggccggtgaa aaaccaggtc cgcacccgcg
     6721 cgccgagact gcgcgccgaa gcggcggcgg cggtttcgga gattttgttc ggcgcaaggg
     6781 tcatcgaaaa gggctcgccc gggagtcaga ccgaccggcc cgctatccgg ccgccacgct
     6841 acaccaaggc gagaccagcc ggaagttatt ggcgaaatcg aggccgcggc gagcgcggcg
     6901 gcgccgttca attcctcgtc agcgcgctcc gccctgcagc ggcgcaaatg acaagcccgc
     6961 ctgaccagcg tattatccgc gtcgtcaagg ccgtcgcgcc tcttgagcct ccgccaacgg
     7021 cgccgcggct cgcggaacaa caacattccg cttggaggtg gtcatggcga atttccacgt
     7081 ctatcttggt caagatgttc gtgctggcct tccggtcatc cgcaaagtcg ccccgagcga
     7141 catcagagac gcgttgatca agggttttga cgatttccgc gccatgccct cgcatcttct
     7201 gttcctcgct ttgatctacc cgctttgcgg cattgtcctt gcctatgtga cgtcgcaaca
     7261 gaacctgttg cagctcctct ttccgctggc gtccggattc gccctggtcg gccccttcgc
     7321 cgcgatcggc ctttacgaga tgagccggcg gcgcgagctc ggcctcgaca cgtcctggcg
     7381 gcatgctttc gaggtgatcc gctcgccgtc cattccttcg atcatcgcgc tgggcctcgc
     7441 cctcgtcgcg attttcgcgc tatggctggc ggcggcgcag gtcctctatt ccttcctcta
     7501 cgggccgatc ccaccctcgt cctatggcgc gttcctcggc gacattttct cgtccggccg
     7561 cggctgggcg ctgatcgtga tcggctgctt tatcggcttc tgcttcgccg tcatcgcctt
     7621 ctcgatcagc gtcgtcgcct tcccgctgct gctcgaccgc gatgtcggcc tcgccgcggc
     7681 gatcggcgcc tccgtcaaat gcgtcctcga caatcccgtt acgatggcgc tgtggggcct
     7741 gaccatcgcc gcgctgctgg cgatcggctc gctgccgctc ttcctcggct tggtggtgat
     7801 catgccggtt ctcggccacg cgacttggca catgtaccgc aaggctgtgg agcgcgaccc
     7861 ggcgcatgag catctcgccg actggcccgc cgaccggatg ggccggccgg cgagccaggt
     7921 cgcctcggcg catgcggtgc tcttcccctg gccgaaatcg gaggggtaaa tttttaaagg
     7981 cgccgccaga cgaccaggcg cttcaggcgc gacgacatga aggctgacga aacgcggtcg
     8041 gcgtcaccgc agcgacgccg caatgtcggc ggtcgacctg aatggaagaa gcggatcatc
     8101 gttcgagggc gcacacggtg acagtgcact tttctctttg gagtcgaaaa aggcgcgcca
     8161 accgccaccc gcactgtctt cccgcaccgc cgcgcgaaca tcattgaatg cgctatcgct
     8221 ataaattgaa tgcgccatca ctttaagcgg caggcaagga gcagttctct cagtatgggt
     8281 caatttcgca gcaaatcgtg acggcggctc atcttgacca agaatgagca ttggtcgttc
     8341 gcgaaggggg cttcagattc taatcgtcgt cctgcaatct ttaccgaggc tcgtctccgc
     8401 aggtgggcct cttcagcgcg gaggcggcga gaggattggc tcagaggccc gtcaatcctc
     8461 tcatagcctg cgaaaatgcg gccgccctgc ggcgtggcgc aaacaagcct tggactctcc
     8521 cgacaaagca cggaggcctg ccgcggctct cgcgttcaca tcgcgcgcag caactccaat
     8581 aggatcagag cggcgactcc gtcgtcgagg ccgcgcgaat atcaattgag cgcaccggta
     8641 cgctgaattg atgagcgcgc ctcgacggga tcgctcagcg caacgacgct gcaatatcgg
     8701 cgatcgtcct gaacagaatg agcgggtcgt ctttcgaagg caggaaacca cgtcgcctcc
     8761 agaacgccgc cgcttccatg tcgacggcgt tgacgatcag cgcgcgacct ccgatcaggc
     8821 tcgcgggcct aagcgcaagt ccataatacc caacgacccg attcacttca tgaacaaacg
     8881 ataacggcgg taaatcctct ttcctgattg gagagcgctc gcgccttgag ccagtggccg
     8941 agagagggtt tgccgcaaga aaatccatcg agagcatgaa ccgccgtcag cggctcaggg
     9001 cgtgaaatcg ccacctcaac gcccgccgcc cgaaccgccg gcctcccacg gcgccgcgcg
     9061 gtgaaagagc tccaccattt ccggcgccgg cgcagccggc tcggacaatg cccgcataaa
     9121 agctttaaaa tcggcgggac tcattcggat gggccgcgtt tccatcaaca catcctcagc
     9181 cgcgcgcacc gccgcatcgc gcacgaaatc cgtgcgcgag cggccgcgca acgttgcggc
     9241 tcgatcgatg agagcgatat cggcttccgg cagtcgcatt gagagcggat gctccttgcg
     9301 tgcggctgtt ttgggcattg aggcctccaa cgacaattaa gaggcgttgt agcgtatttt
     9361 gtggaggcgc a
//
LOCUS       DATGJW010000525         4860 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_929775, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000525 DATGJW010000000
VERSION     DATGJW010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4860)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4860)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4860
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_929775"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..634)
                     /locus_tag="VKU03_15940"
     CDS             complement(<1..634)
                     /locus_tag="VKU03_15940"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72807.1"
                     /translation="MAIALSHAGGGVDASAPPVLRIEGLSKSFGGAQALDHVSLTVRQ
                     GEVHGLLGQNGSGKSTLIKILSGFHHPDGSPTIEIDGRPVSLPVSLAALRANGVSFVH
                     QHLGLLPSLTVLENLLLSDFAAENRWYVDWRAEARRARSLFERYDIRLDPTSPLAALT
                     PVERAQLAIVRAFDEVTRRKGQALTRGLLVLDEPTPFLPAQDVDALFKLIR"
     gene            complement(619..1719)
                     /locus_tag="VKU03_15945"
     CDS             complement(619..1719)
                     /locus_tag="VKU03_15945"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ72808.1"
                     /translation="MKSSIAAACVIAGIQLFAGAAFAESPEAAQAQKQLEPYRKLPTF
                     QPPGEAFDAAVCMKGKSILSLPASSAVPFIKTIQASMSKVAKEIGFNLKIWENQGQPT
                     QWVQGFDYAINNKFNLIDLLAGADPRFLEPQVKAAEAAGLKVVASHLTGYEQPLPGGV
                     TGVVPIDYKRAGALLADWAIWKTDGKVNAIIVGINDVLSTDSMMSGVKEEFAKCPNCK
                     STYVNVTIPDMATKTQTNVQAALTADPTVNYVIPIYDVLTQWVVPAVTITGRTDKVKV
                     ATFNGTPFAIGMVQEGKIEVDIGENLDWIGHAVVDSEMRMLCGLPTVKDPKIPLLIFD
                     KSNADTAGKPPQVNTGYGDAYIEGYRKLWRLH"
     gene            1882..3126
                     /locus_tag="VKU03_15950"
     CDS             1882..3126
                     /locus_tag="VKU03_15950"
                     /inference="COORDINATES: protein motif:HMM:TIGR01879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M20 family metallo-hydrolase"
                     /protein_id="HLJ72809.1"
                     /translation="MQGVDEDRAIADLRTLARFGKAGTGVNRPALSDADLEAREWLRA
                     RMAEAGLEAAIDGLGNVFGRAPRARAAVLLGSHSDSVPNGGWLDGALGVVFALEVARA
                     RQATQPDAAIGVDAIAFADEEGTFLACAGSRSFCGALAPEELEKARNPAQERLADRLR
                     DLGLSGRPLARLDCERHRAFLEAHIEQGPRLIESGVEIGVVAGIVGLRRRRVRFEGQA
                     DHAGTTPMAMRRDAGLAMHAFAVAAAETLRAAGSEDSVWNFGVVSVKPGAANVVPDDA
                     EIVVEFRDLSTAVMNRMDEALKALIAERDGAAGVRVTSATIGEIEPSLMDERLIARIE
                     AAADREGARRQRMQSGAGHDAMIVGRRIPAAMLFVPSIGGRSHHVSEDTDEKDIRRGL
                     RVYARAAQSILEAIEPDGQGPL"
     gene            3152..>4860
                     /locus_tag="VKU03_15955"
     CDS             3152..>4860
                     /locus_tag="VKU03_15955"
                     /inference="COORDINATES: protein motif:HMM:NF014071.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HLJ72810.1"
                     /translation="MKMIGVDVGGTFTDIVYCDMSSGKLAVHKVSTTPDDPSRAIMQG
                     VAEICAENGVAPDTIDYVLHGTTTATNAVLEHKGARTGMVTNQGFRDIVHIARHQRVE
                     HYSIMQELPWQNRPLAPRRHRKVVKGRLIPPHGEELEPLDEADVVAAARALKAEGVEA
                     VAVCFLFSYLNPAHEDQAKRILARELPGVFIATSSSVSPQFREFERFTTAALCAFVGP
                     KMRAYIGKLDASLREAGLGADLRIMASNGGVATPAMAEEKPVMTLLSGLAAGVLGGAW
                     VGELSGRRKLITFDIGGTSADIGLVVDGRYAETDPRSTSIAGFPLLLPMIDIHTIGAG
                     GGSIAYIDQGGAFRVGPRSAGAAPGPAAYGRGGEQPTVTDANVVLGRLDKDDFLGGGM
                     KLDARAADRVIDELAGRLGLGKLEAAEGVLTVINSNMANAIRSRTVQKGVDPREFALV
                     AFGGAGPLHGAEVARMLGIPEVIVPPYPGITSAMGLLTTDLKYDTIRTQFQVSGKIDL
                     DRLNADLKEMERRLAAQFAADHLDADKFRFVRDGDLRYVGQGYELKISFPDGTLAAAD
                     LEA"
BASE COUNT          817 a         1607 c         1614 g          822 t
ORIGIN      
        1 cgcggatcag cttgaacagc gcgtcgacgt cttgcgccgg caggaacggc gtcggctcgt
       61 cgagcacgag cagaccgcgc gttagggcct gccctttgcg cctcgtcacc tcgtcgaagg
      121 cgcggacgat cgcaagctga gccctctcga ccggggtcaa ggccgccagc ggactggtcg
      181 gatcgagacg gatgtcatag cgctcgaaca ggctccgagc gcgccgcgcc tcggcgcgcc
      241 agtcgacgta ccagcgattt tcggcggcga aatcggagag aagcagattt tcgaggacgg
      301 tcagcgacgg caacagcccg agatgctgat ggacgaagct gacgccgttg gcgcgaaggg
      361 cggccaagct taccggaagg gagacagggc gcccatcaat ctcgatcgtc ggcgatccgt
      421 ccggatggtg aaagccggac aggatcttga tcagggtgga cttgcccgat ccgttctgtc
      481 cgagcaagcc atgcacctcg ccttgccgga ccgtcaggct gacatggtcc agggcctgcg
      541 cgccgccgaa gctcttcgac aggccttcga tccgaagaac cggaggcgcc gaggcgtcga
      601 cgccgccgcc ggcatggctc agtgcaatcg ccacagcttg cgatatccct cgatatacgc
      661 gtcgccatag ccggtattga cctggggcgg cttgccggcc gtgtcggcgt tgctcttgtc
      721 gaaaatgagc agagggattt tggggtcttt caccgtgggc aggccgcaaa gcatgcgcat
      781 ctcggaatcg acgacggcgt ggccgatcca gtcgagattt tcgccgatat cgacctcgat
      841 cttgccttcc tgcaccatgc cgatggcgaa aggcgtgccg ttgaaggtgg cgactttcac
      901 cttatccgta cggccggtga tcgtcaccgc cgggacaacc cattgagtca gcacgtcgta
      961 gatcgggatc acataattga cggtcggatc ggcggtcaag gcggcctgaa cgttggtctg
     1021 cgtcttcgtc gccatgtcgg ggatcgtcac gttgacatag gtcgacttgc agttcgggca
     1081 tttggcgaac tcttccttga cgccgctcat catggaatcg gtcgaaagca cgtcgttgat
     1141 gccgacgatg atcgcattga ccttgccgtc ggtcttccag atcgcccaat cggcgagcag
     1201 cgccccggcg cgcttgtagt cgatcggcac aacgccggtc acgccgccgg gaagcggttg
     1261 ctcatagccg gtcagatgcg aggcgacgac tttgagcccg gccgcctccg ccgccttgac
     1321 ctgcggttcg aggaagcgcg gatcggcgcc cgccagcaga tcgatcagat tgaacttgtt
     1381 gttgatcgcg tagtcgaagc cctgcaccca ttgcgtcggc tgaccctggt tctcccatat
     1441 tttcaaattg aagccgatct ccttcgcgac tttgctcatg ctcgcttgga tcgtcttgat
     1501 gaacggcacc gcgctcgaag cggggaggga gaggatcgac ttgcccttca tgcagacggc
     1561 ggcgtcgaac gcttcgcccg gcggctggaa ggtcgggagc ttccgatagg gctcgagttg
     1621 cttctgggcc tgggcggctt cgggactttc cgcgaaagcc gctcccgcga aaagctgaat
     1681 tcccgcgatg acgcacgcgg ccgcgatgct gctcttcatt gggtctcctc ccctcgtcgc
     1741 tggcgacgaa ccgcgcgagc ccgatcgccc gggccggatc aatgcccgac agccctggac
     1801 atggaaatat caattatgta ttcgaaatgc aatctttgcg cgaaggacgc gcagcgagcg
     1861 aagtcgaatg ggagcggcgg catgcagggc gtggacgagg atcgcgcgat cgccgacctg
     1921 cgtacgctcg cccgcttcgg caaggcgggg acgggcgtca accgccccgc tttatcggac
     1981 gccgatctcg aagcgcgcga atggctgcgc gcgcgcatgg ccgaagccgg ccttgaggcg
     2041 gcgatcgacg gattgggcaa tgtcttcggc cgagcgccgc gcgcccgcgc ggctgtgctt
     2101 ctcggctcgc attccgacag cgttccgaac ggcggctggc tcgacggcgc gctcggcgtc
     2161 gtgttcgccc tcgaagtcgc gcgcgcccgc caggcgacgc aacccgacgc ggcgatcggc
     2221 gtcgatgcga tagccttcgc cgacgaggaa gggacttttc tcgcctgcgc cggaagccgc
     2281 tccttctgcg gcgcgctcgc gcccgaagag ctggaaaagg cgcggaaccc ggcgcaagag
     2341 cggctggccg accgcctccg cgatcttggc ctttccggcc gccccttggc gcggcttgac
     2401 tgcgaacggc accgcgcctt cctcgaagcg catatcgaac aggggccgcg cctgatcgaa
     2461 agcggcgtcg aaatcggcgt cgtcgccggc attgtcggct tgcgccgccg ccgtgtgcgc
     2521 ttcgaggggc aagccgatca cgcaggcacg acgccgatgg cgatgcgccg cgacgccgga
     2581 ttggcgatgc acgctttcgc cgtcgccgcc gccgagacgt tgcgagccgc cggttccgaa
     2641 gattccgtct ggaatttcgg cgtcgtctcg gtgaagccgg gcgccgccaa tgtcgttccc
     2701 gacgatgctg aaatcgtggt ggagttccgc gatctttcga ccgccgtgat gaaccgcatg
     2761 gacgaggcgc tgaaggcgct catcgccgaa cgggacggcg ccgccggcgt tcgcgtaacc
     2821 tccgcgacga tcggcgagat cgagccgagc ctgatggacg agcgcctcat cgcccgcatc
     2881 gaggccgccg ccgatcggga aggcgcgcgc cgccagcgca tgcagagcgg cgccgggcat
     2941 gacgcgatga tcgtcggccg gcgcattccg gcggcgatgc tgttcgtccc cagcatcggc
     3001 ggcaggagcc atcacgtttc cgaggacacc gacgagaagg acatacgccg cggcctccgg
     3061 gtctatgcgc gggccgcgca atccatcctg gaagcgatcg agccggacgg ccaggggccg
     3121 ctgtgagcga agttcgacga aagaggctac gatgaaaatg atcggcgtgg atgtcggggg
     3181 cacgttcacc gacatcgtct attgcgacat gtcgtccggc aaactcgccg tccacaaggt
     3241 ctcaaccacg cccgacgacc cttcccgggc gatcatgcag ggcgtcgccg agatttgcgc
     3301 cgagaacggc gtcgcgcccg acacgatcga ttacgtgctg cacggcacga cgaccgcgac
     3361 caacgccgtt ctcgagcata agggcgcgcg caccgggatg gtgaccaacc agggctttcg
     3421 cgacatcgtc catatcgctc gccatcagcg cgtcgagcac tattcgatca tgcaggaatt
     3481 gccatggcag aaccggccgc tggcgccgcg ccgccatcgc aaggtcgtca agggccggct
     3541 gattccgccg catggcgaag agttggagcc gctcgacgaa gcggacgtcg tcgccgcggc
     3601 gcgcgcgctg aaggcggagg gcgtcgaagc ggtcgccgtc tgcttcctct tctcctatct
     3661 caatcctgcg catgaggacc aggccaagcg catcctggcg cgcgaattgc ccggcgtgtt
     3721 catcgccacc tcgtcctccg tctcgccgca atttcgcgag ttcgagcgct tcaccacggc
     3781 ggcgctctgc gcctttgtcg gcccgaagat gcgcgcctat atcggcaagc tcgacgcctc
     3841 gctgcgcgag gcggggctcg gcgccgatct ccgcatcatg gcgtcgaatg gcggcgtcgc
     3901 cacgccggcc atggccgagg aaaagccggt catgactctg ctctcgggcc tcgccgccgg
     3961 cgtgctgggc ggcgcctggg tcggcgagct cagcggccgg cgcaagctga tcacgttcga
     4021 catcggcggc accagcgccg atatcggcct ggtggtcgac ggccgctacg ccgaaaccga
     4081 tccgcgcagc acgtccatcg ccggctttcc gctcctgctg ccgatgatcg acattcacac
     4141 gatcggcgcc ggcggcggat cgatcgccta tatcgaccag ggcggcgcgt tccgggtcgg
     4201 gccgcgcagc gccggcgccg cgcctgggcc ggccgcgtac ggccgcggcg gcgaacagcc
     4261 gacggtgacg gacgccaatg tcgtgcttgg acggctcgac aaggacgatt ttctcggcgg
     4321 cggcatgaag ctcgacgcgc gcgccgccga ccgcgtgatc gacgagcttg ccgggcgcct
     4381 cggcctcggc aagctggagg cggcggaagg cgtgctcacc gtcatcaaca gcaacatggc
     4441 caacgccatc cgctcgcgaa cggttcagaa aggcgtcgat ccgcgcgaat tcgcgctcgt
     4501 ggcgttcggc ggcgccggcc cgctccacgg cgcggaagtg gcgcggatgc tgggcattcc
     4561 ggaagtgatc gttccgcctt atccgggcat cacctccgcc atggggctgc tcaccaccga
     4621 tctcaaatac gacacgatcc gcacccagtt ccaggtgagc ggcaagatcg atctcgatcg
     4681 gctcaacgcc gatttgaagg agatggagcg ccggctcgcc gcgcaattcg ccgcggacca
     4741 tctcgacgcg gacaagttcc gcttcgtacg cgacggcgat ctgcgctacg tcggccaggg
     4801 ctacgagctc aagatttcct tccctgacgg gacgctcgcc gcggccgatc tcgaggcgat
//
LOCUS       DATGJW010000526         7482 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1450368, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000526 DATGJW010000000
VERSION     DATGJW010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 7482)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7482)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7482
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1450368"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VKU03_15960"
     CDS             <1..189
                     /locus_tag="VKU03_15960"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018900979.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3830 family protein"
                     /protein_id="HLJ72811.1"
                     /translation="LYPGGISETEILLAYGGVHFACKMGQLAGNHFITLTSGLENLAK
                     LGKMTLWEGAQKIRFERA"
     gene            complement(283..846)
                     /locus_tag="VKU03_15965"
     CDS             complement(283..846)
                     /locus_tag="VKU03_15965"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003562843.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="isochorismatase family protein"
                     /protein_id="HLJ72812.1"
                     /translation="MSADAALLVIDAQESFRRRPYWSDADAPLFLERLQALIDGAKAR
                     GMPIVQIFHVEESGDFSLASGHVTALAPLKIAPDAVFHKRRHSALIGTGLDVWLTQRG
                     VRRLIVSGIRTEQCCETTTRHASDLGYPVDFVSEATLTFPMTDAGGRSFSPAEIKART
                     ELVLAGRFARIATVEEALASRTAARAA"
     gene            983..1966
                     /locus_tag="VKU03_15970"
     CDS             983..1966
                     /locus_tag="VKU03_15970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004673375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72813.1"
                     /translation="MKALVTGSAGHLGEALVRALRGAGHETTGLDIRPSPFTTHVGSI
                     VDRAFVARCMKGVERVFHPATLHKPHVATHRRQDFIDVNVSGTLNLLEEAATAGVKSF
                     VFTSTTSVFGDALAPPPGAPAAWVTEEVAPFPKNIYGVTKAAAEDLCQLFHRNQKLAC
                     LVLRTSRFFPEADDSRDVREAYADENVKANEFLFRRVDIEDVVGAHLLAAERAPEIGF
                     GKYIVSATSPFLPEDLAELRRDAPAVARRRAPGYEKEYERRGWRMFPSIGRVYVNEKA
                     RRELDWRPRHDFAALIGRLAQGGDMRSPLAQAIGSKGYHAEAFAEGPYPVE"
     gene            2047..2487
                     /locus_tag="VKU03_15975"
     CDS             2047..2487
                     /locus_tag="VKU03_15975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012555342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="EVE domain-containing protein"
                     /protein_id="HLJ72814.1"
                     /translation="MRKYWIVVAAAEHVRRGLAGAFVQACHGKATPLKRMKAGDGIVC
                     YSPTEAFRGAGRLQAFTALGFLREREPYIADMGQGFRPARRDVAWMEGEAAPLAPLLE
                     TLQLTAGRRNWAYPFRFGVVEIGEPDFRLIAAAMTANAMAAAAA"
     gene            complement(2513..2950)
                     /locus_tag="VKU03_15980"
     CDS             complement(2513..2950)
                     /locus_tag="VKU03_15980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012653256.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HLJ72815.1"
                     /translation="MIDSRTAPYGLFGLRLALGILFLAHASLKIFVFTPAGFAHFFGM
                     LGLPAWFAYLTILWEALGGAALILGVWPRLAALAVAPILLGAIAFVHGSAGFFFTDPN
                     GGWEFPALWTVGLVVLALCGDGAFALVPTPLAQFGRDFARRAA"
     gene            complement(3089..3997)
                     /locus_tag="VKU03_15985"
     CDS             complement(3089..3997)
                     /locus_tag="VKU03_15985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018896940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0001216 - DNA-binding transcription
                     activator activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ72816.1"
                     /translation="MDRLDCDRAFVAVLETGSFSRAASRMGVSSGQASKLVARLEADL
                     GVRLINRTTRALSATEAGQAYYERLKGLIEEFDALDAAVRNVAGAAKGRLRVTAPLTF
                     GTMRLAPDLIEFAETYPDIELDVNFTDRIANLIDEGFDMAVRIGGPADSSLVARRLCE
                     VGIVTAASPAYLKAHKAPKAPADLAGHACIVDTNFRDPLVWRYRVNGETIGVPVKPRA
                     RFSNAEACLRAGEAGLGVVRLPNFAVGRSIARGALIPLLASFATEPGGAFALYPPGRG
                     LALKVRVLVDFLAARYRGGAVWESED"
     gene            4311..5282
                     /locus_tag="VKU03_15990"
     CDS             4311..5282
                     /locus_tag="VKU03_15990"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011160111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1C family serine protease"
                     /protein_id="HLJ72817.1"
                     /translation="MVEKSRLDAPPTQPQHDDLHFDLDRALKSVVALQAKIPDDAFTA
                     GILGSERSGSGVVIRDSGLVVTIGYLITEAESVWLTAADGRVVPAHPMAYDQETGFGL
                     VQALGPLDLPALEIGRMSEAAIGDPVVVAGGGRAPITRALIVGKQEFAGYWEYLLDEA
                     VLTAPAHPFWGGAGVISNSGKLLGIGSLQVQQVTEKGQQYDINMIVPIDLLPPILDDL
                     LKFGRVNKPPRPWLGVYSGESQGRIIVADVAEDGPAAAAGLRRGDIISAVRDSSVEDL
                     ADFYRKVWACGPAGVEIPMEIIRDGRSAWARIKTVDRASLLKKPRLQ"
     gene            complement(5453..6193)
                     /locus_tag="VKU03_15995"
     CDS             complement(5453..6193)
                     /locus_tag="VKU03_15995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004122054.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase"
                     /protein_id="HLJ72818.1"
                     /translation="MAKAKPVSAGAAPAETRVARYDWRALTDELGAYGAAVLPTLLTA
                     EECVAIAASYPDETRFRSHIVMARHGFGKGEYRYFKYPLPSLVAELRSALYPRLAPIA
                     NEWSERMGEETRYPAEHEAFLKLCHRAGQTRPTPLLLQYVPGDFNCLHQDLYGDLAFP
                     VQVAILLSEPGRDFTGGEFVMTEQRPRMQSRVEVVPLRQGDAVAFAVHNRPAKGTKGY
                     YRVNMRHGVSRLRSGRRHTLGIIFHEAR"
     gene            complement(6199..7284)
                     /gene="ada"
                     /locus_tag="VKU03_16000"
     CDS             complement(6199..7284)
                     /gene="ada"
                     /locus_tag="VKU03_16000"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859361.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional DNA-binding transcriptional
                     regulator/O6-methylguanine-DNA methyltransferase Ada"
                     /protein_id="HLJ72819.1"
                     /translation="MEFPAKPARAPALPIERDPRWAALLARDPAAEGAFVYSVATTGV
                     YCRPTCPSRRPKPENVTFYASPTEAENAGFRPCRRCRPNEAPAAERHAALVAEACRRI
                     ERAESPPSLDALAAKAGVSPSHFHRLFKSITGLTPKAYAAAHRAKRVREELSNGASSV
                     TEAIYGAGFNASSRFYEKAKDALGMTPSAYRAGGAGAEIKFAIGQCSLGAILVARSEK
                     GVCAILLGEDPEELVRDLQDRFPKAKLIGGDEEFEKLVAKVVGFVEAPALGLDLPLDV
                     RGTAFQQRVWEALRKIPAGATASYAEIAERIGAPKAVRAVAAACAANKIAVAIPCHRV
                     VRKDGDLSGYRWGVARKRALLAKEQAA"
BASE COUNT         1289 a         2475 c         2528 g         1190 t
ORIGIN      
        1 ctctatcccg gcggcatcag cgagacggaa atcctgctcg cctatggcgg cgtgcatttc
       61 gcctgcaaga tgggccagct cgccggcaat catttcatca cgctcacgtc cggattggag
      121 aaccttgcca agctcggcaa gatgacgttg tgggaaggcg cgcagaaaat caggttcgag
      181 cgggcgtagc cgtcattgcg acgagcggag cgacgaagca atctattcac agggcgtcgc
      241 ccttcgagtg gattgcttcg cggcgctcgc aatgacgttc gctcacgccg cgcgcgcggc
      301 ggtccggctg gccaaggctt cttcgacggt cgcgatgcgg gcgaagcggc cggcaaggac
      361 gagctcggtc cgcgccttga tctcggccgg gctgaaactc cgcccgccgg cgtcggtcat
      421 agggaaagtc agcgtcgcct cgctgacgaa gtcgaccgga tagccgagat cggaggcgtg
      481 gcgcgtcgtc gtctcgcagc attgctcggt acgaatccct gagacgatca ggcgccgaac
      541 gccgcgttgc gtcagccaga catcgagccc ggtgccgatg agcgcgctgt ggcggcgctt
      601 gtggaagacc gcgtccggcg cgatcttcaa aggggcgagt gcggtcacat ggcccgaggc
      661 gagcgaaaag tcgccgctct cctcgacatg gaatatttgc acgatcggca tgccgcgcgc
      721 cttggcgcca tcgatcagcg cttggagccg ctccaagaac aaaggcgcat cggcgtcgct
      781 ccaatagggg cggcggcgaa acgattcctg ggcgtcgatg acgagcaggg cggcgtcggc
      841 ggacattttt gggctccttc ggtgcttgcg cgatgatcat agccgcgcca agaagcgtgg
      901 caaatgcggg tggcggaccg gagtcgccgc tgtcattccc gcggaagcct ccacgcctta
      961 gaactgacat agagttgaag ccatgaaagc tctggtcacg ggaagcgccg gccatctcgg
     1021 cgaagctctg gttcgcgcct tgcgcggcgc cggccatgag acgacgggac tcgacatccg
     1081 gccttcgcct ttcacgactc atgtcggctc gatcgtcgac cgcgcctttg tcgcgcgctg
     1141 catgaaaggc gtcgaacggg ttttccatcc ggcgacgttg cacaagccgc atgtcgcgac
     1201 gcatcggcgg caggacttca tcgacgtcaa tgtcagcggg acgctcaacc tcttggagga
     1261 agcggccacg gccggcgtga aatccttcgt cttcacctcg acgaccagcg tcttcggcga
     1321 tgcgctcgcg ccgccgccag gcgcgccggc cgcctgggtg accgaagagg tcgcgccgtt
     1381 cccgaagaac atctatggcg tgaccaaggc cgctgccgag gacctttgcc aattgtttca
     1441 ccgcaaccag aagctcgcct gcctggtttt gaggacgtcg cgcttctttc ccgaagcgga
     1501 cgacagccgc gacgtgcgcg aggcttatgc cgacgagaac gtcaaggcca acgaatttct
     1561 ttttcgccgc gtcgatatcg aggacgtggt cggcgcgcat cttctcgccg ccgagcgcgc
     1621 gccggagatc ggcttcggca aatatatcgt cagcgcgacg tcgccgttcc tgcccgagga
     1681 ccttgccgag ttgcgccgcg acgcgccggc cgtcgcgcgg cggcgcgcgc ccggttacga
     1741 gaaagagtac gaacgcagag ggtggaggat gttcccgagc atcgggcgcg tctatgtcaa
     1801 cgaaaaagcg cgccgcgagc ttgattggcg gccgcgccac gatttcgcgg cgctgatcgg
     1861 ccggcttgct caaggcggcg acatgcgcag cccgctggcc caggcgatcg gctcaaaggg
     1921 ctatcacgcc gaggctttcg ccgaggggcc gtatccggtc gagtgacgag gcgattttcg
     1981 ctcctgccaa aatctggcgc cggcgcggct taagagctgc gcatacgagc ccgacaggag
     2041 agtcgcatgc gcaaatactg gatcgtcgtc gctgccgccg agcatgtccg gcggggcctc
     2101 gccggcgcct tcgtgcaggc ctgccacggc aaggcgacgc cgctgaagcg gatgaaggcg
     2161 ggcgatggaa tcgtctgcta ctcgccgacc gaagcgtttc gcggcgccgg ccggctgcaa
     2221 gccttcaccg cgctcggctt cttgcgcgag cgcgagccct atatcgccga catgggtcaa
     2281 ggcttccggc cggcgcggcg cgacgtcgcc tggatggagg gggaagccgc gccgctcgcg
     2341 ccgctgctcg agacgttgca attgacggcc ggccgccgaa actgggccta cccgtttcgc
     2401 tttggcgtcg tcgagatcgg cgaaccggat tttcggctga tcgccgcggc aatgacggcg
     2461 aacgcgatgg ccgccgcggc ggcctaagcc acgctccgtt cgatgcgtcc gatcacgcgg
     2521 cgcggcgggc gaaatcgcgg ccgaattggg cgagcggcgt cgggaccagc gcgaaggcgc
     2581 cgtcgccgca cagcgccagg acgacaaggc cgaccgtcca caaagccggg aattcccagc
     2641 cgccattcgg gtcggtgaag aagaagccgg ccgaaccatg cacgaaggcg atggcgccga
     2701 gcaggatcgg cgccacggcg agcgccgcga ggcgcggcca gacgccgaga atgagcgcgg
     2761 cgccgccgag cgcttcccaa aggatggtca gataggcgaa ccaggccggc aggccgagca
     2821 tgccgaagaa atgcgcgaag ccggccggcg tgaagacgaa aatcttcaac gaagcgtgcg
     2881 ccagaaatag gattccaaga gcgaggcgga ggccgaaaag gccatagggc gctgtgcggg
     2941 agtcgatcat cgtcattgcc tttctgatcg gagccgaaag gcgggcgcga ggcgctcgcc
     3001 gccgcggcgt cagcctgata cgcagaataa gcgtttccga tcgcgttatt acgccgccga
     3061 tttggcaatg ttcctttcca aaacggaact aatcctcgga ttcccaaacc gcgccgccgc
     3121 gatagcgggc ggcgagaaaa tcgaccagaa cccggacttt caaagcgagc cctcggcccg
     3181 gcggatagag ggcgaaggcg ccgcccggtt cggtggcgaa actcgccaaa agcggaatca
     3241 acgcgccgcg cgcgatgctg cgccccaccg cgaaattcgg caggcgaacc acgccgagcc
     3301 cggcttcgcc ggctctgagg caggcctcgg cgttcgagaa ccgcgcccgc ggcttgaccg
     3361 gcaccccgat cgtctcgcca ttgacgcgat agcgccagac cagcggatcg cggaaattgg
     3421 tgtcgacgat gcaggcatgt ccggccaggt cggccggcgc cttgggcgct ttgtgggcct
     3481 tcaaataagc cggcgaggcg gcggtgacga tgccgacctc gcacagccgc cgcgcgacga
     3541 ggctcgaatc ggccgggccg ccgatccgca ccgccatatc gaagccttcg tcgatcaggt
     3601 tagcgatgcg gtcggtgaag ttcacatcga gttcgatgtc gggataagtt tcggcgaatt
     3661 cgatcaggtc gggggcgagc cgcatcgtgc cgaaggtcaa tggcgcagtg acgcgcaagc
     3721 gccctttggc cgcgcccgcc acgttgcgca cggccgcgtc gagcgcgtcg aactcctcga
     3781 tcagcccttt cagacgctcg taataggcct ggccggcttc ggtcgcggac aacgcgcgcg
     3841 tcgtgcggtt gatcaggcgc acgccgagat cggcttcgag cctcgccacg agtttcgacg
     3901 cctggccgga actgacgccc atccgcgaag cggcgcgcga gaagctgccg gtctccagga
     3961 ccgcgacgaa agcgcggtcg caatccaatc ggtccattta aacattccaa accggaacgc
     4021 cggcggttat cgtgtcacgg attgcatccg caatggaagg ggcggcgatc ccccgccagc
     4081 gatggaacgg ccggccgcgc ggcgaattga ggttggcgat ccgatcctgc cgcgccaagc
     4141 gccgagacac tcgcgaaccg gccgcctccg cttttcggcg accgacgtac ttcggcgaac
     4201 gcgccaatcc ggcggaaagc gccaaaattg cgatccttgc gcccgcgcgt tagcgttcta
     4261 tctaagcgga agaggcggcg gcgaaaaata gaaaaaatag gatgacagcg atggtcgaga
     4321 aatcccgttt ggacgcgccg ccgacccagc cgcagcacga cgacttgcat ttcgatctcg
     4381 accgggcgct gaaatccgtg gtcgcgctcc aggccaagat accggatgac gcgttcaccg
     4441 ccggcattct cggctcggag cgctcgggca gcggcgtcgt cattcgcgac agcggcctcg
     4501 tcgtgacgat cggctatctg atcacggagg ccgaaagcgt ctggctgacg gccgccgatg
     4561 ggcgcgtcgt tccggcgcat ccgatggctt acgaccagga gaccggcttc ggcctcgtcc
     4621 aggctttggg cccgctcgat ttgccggcgc tggagatcgg ccggatgagc gaggcggcga
     4681 tcggcgatcc ggtcgtcgtc gcgggcggcg gcagggcgcc gatcacccgc gccttgatcg
     4741 tcggcaagca ggaattcgcc ggctattggg aatatctgct ggacgaagcc gtgctcaccg
     4801 cgccggccca tccgttctgg ggcggcgccg gcgtgatcag caacagcggc aaactgctcg
     4861 gaatcggctc gctgcaagtc cagcaagtga cggaaaaggg ccagcaatac gacatcaaca
     4921 tgatcgtgcc gatcgatctc ctgccgccga tcctggacga tctcttgaaa ttcggccgcg
     4981 tcaacaagcc gccgcggccc tggctcggcg tctattccgg cgaaagccag ggccgcatca
     5041 tcgtcgccga cgtggccgag gacgggccgg ccgccgccgc cggtctgcgc cgcggcgaca
     5101 tcatctccgc ggtgcgcgat tcgagcgtcg aggacctcgc cgatttctat cgcaaggtct
     5161 gggcctgcgg cccggccggc gtcgagattc cgatggagat tatccgcgac ggccgcagcg
     5221 cctgggcgcg catcaagacc gtggaccgcg ccagcctgct gaagaagccg cgcctgcagt
     5281 aaattcctcc cccgcgcagc gggggagggg aaccatgcga agcatggtgg agggggctcc
     5341 ggcggttgag gattatcctg agagaccgaa gccccctcca cgcgctgacg cgcggtctcc
     5401 ctcccccgtc ttcgacgggg aggacatcgc gcgcgacgcc atgggagcgc gctcaccgcg
     5461 cctcgtggaa gatgattccc agcgtgtgcc ggcgcccgga ccgcaggcgg ctgacgccgt
     5521 ggcgcatgtt cacgcgataa tagcctttcg tacctttcgc cggccggtta tgcaccgcga
     5581 aggccaccgc gtcgccttgc cgcagcggga cgacctcgac gcggctttgc atgcgcgggc
     5641 gctgctcggt catcacgaac tcgccgccgg tgaagtcccg tcccggctcg gagagcagga
     5701 tcgcgacctg cacgggaaag gcgaggtcgc cgtaaaggtc ctgatgcagg caattgaaat
     5761 cgcccggaac gtactgaagc agaagcggcg tcggccgggt ctggccggcg cgatggcaga
     5821 gcttcaggaa tgcctcgtgc tcggccggat agcgcgtctc ttcgcccatc cgctcgctcc
     5881 actcgttcgc gatcggggcg aggcgcggat agagggctga gcgcagttcg gcgacgaggg
     5941 acggcagcgg atatttgaaa tagcggtact cgcctttgcc gaagccatgc cgcgccatga
     6001 cgatatggct gcggaaacgc gtttcgtcgg gatacgaagc cgcgatcgcc acgcattcct
     6061 cggcggtgag cagcgtgggg agaaccgctg cgccatacgc gccgagttcg tccgtcagcg
     6121 cgcgccaatc gtagcgcgcg acacgcgttt cggccggcgc cgcgccggcc gaaacgggct
     6181 tcgccttggc cagcgcgctc acgccgcctg ctccttggcg agcagggcgc gcttgcgcgc
     6241 cacgccccag cgatagccgg aaagatcgcc gtccttgcgc accacccgat ggcagggaat
     6301 cgccaccgcg attttgttcg ccgcgcaggc cgccgccacc gcgcgcaccg ctttcggcgc
     6361 gccgatgcgt tcggcgatct cggcatagct cgccgtcgcg ccggccggga ttttccgcaa
     6421 cgcctcccag acgcgctgct ggaacgccgt gccgcgcacg tcgagcggca gatcgaggcc
     6481 gagcgccggc gcctcgacga agccgacgac cttggcgacc agtttttcga actcctcatc
     6541 gccgccgatc agcttggctt tcggaaagcg gtcctgcagg tcgcggacca gttcttccgg
     6601 atcctcgccg agcaggatgg cgcagacgcc cttctcgctg cgcgcgacga ggatcgcgcc
     6661 gagcgaacac tggccgatgg cgaatttgat ttccgctcca gcgccgccgg cgcggtacgc
     6721 cgagggcgtc atgccgagcg cgtccttggc cttttcatag aagcggctcg acgcgttgaa
     6781 gccggcgcca tagatcgcct cggtcacgct actcgcgccg ttgctcaact cctcgcgcac
     6841 ccgtttggcg cgatgggccg cggcataggc tttcggcgtc aggccggtga tcgatttgaa
     6901 caggcggtgg aagtgggagg ggctcacccc cgccttggcg gcgagcgcgt cgaggctggg
     6961 cggggactcg gcccgttcga tccgccggca cgcttcggcg accaaagccg cgtggcgctc
     7021 ggccgccggc gcctcgttcg gccggcatcg ccggcaaggc cgaaaccccg cgttttccgc
     7081 ctcggtcggc gacgcgtaaa acgtcacatt ctccggcttc ggccgccgcg acgggcaggt
     7141 cggccggcaa tagacgccgg tcgtcgccac cgaatagacg aacgcgccct ccgccgccgg
     7201 atcgcgggcg agcaaagcgg cccagcgcgg gtcgcgctcg atcggcaggg cgggggcgcg
     7261 ggccggcttg gcggggaatt ccatgacgag gctcattgta gcgattcctt cgtgtctgtg
     7321 tcgccctgga gataggcgct cccggcgctc cccgcactcc gcctcttgct ttcaaattcc
     7381 cgcccgtcat ggccgggctt gtcccggcca tccacgcccc gatgacgcgc gcaaccgcgc
     7441 ggagaattag cgactctgcg caaatggcat tcggcgaccg tt
//
LOCUS       DATGJW010000527         2746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1551165, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000527 DATGJW010000000
VERSION     DATGJW010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2746
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551165"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(146..676)
                     /gene="nusG"
                     /locus_tag="VKU03_16005"
     CDS             complement(146..676)
                     /gene="nusG"
                     /locus_tag="VKU03_16005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003597503.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination
                     protein NusG"
                     /protein_id="HLJ72820.1"
                     /translation="MSMRWYIVHAYSNFEKKVAESLKEQAAQRGLAEKFEEIMVPTEQ
                     VVEVRRGRKVSSERKFFPGYVLVKCDLTDDVYHLIKNTPKVTGFLGADKKPMPIPDVE
                     AERIKGQVAEGVERPKSSIRFDIGEQVRVADGPFASFSGVVEEIDEARSRVKVAVSIF
                     GRATPVDLEFGQVEKM"
     gene            complement(734..931)
                     /gene="secE"
                     /locus_tag="VKU03_16010"
     CDS             complement(734..931)
                     /gene="secE"
                     /locus_tag="VKU03_16010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018406587.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecE"
                     /protein_id="HLJ72821.1"
                     /translation="MTNPFEFIQQVRAEGAKVVWPSRRETLITTGLVLLMVVLASLFF
                     LTVDEIIHFAIGFALGIGRHG"
     gene            complement(1182..1535)
                     /locus_tag="VKU03_16015"
     CDS             complement(1182..1535)
                     /locus_tag="VKU03_16015"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008972965.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1236 domain-containing protein"
                     /protein_id="HLJ72822.1"
                     /translation="MSKAACLATLIAATPATAYAQEWVVETPVPPVVAPGAVGLAPPA
                     YYVPPGPVYAAPVYPAPAPWTAERVVVGDVLPPGVAYYPAPPQYGVTEYRYAIVNDEP
                     VLVEPRTRRVIEVLD"
     gene            complement(1718..1793)
                     /locus_tag="VKU03_16020"
     tRNA            complement(1718..1793)
                     /locus_tag="VKU03_16020"
                     /product="tRNA-Trp"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1758..1760),aa:Trp,seq:cca)
     gene            complement(1846..2166)
                     /locus_tag="VKU03_16025"
     CDS             complement(1846..2166)
                     /locus_tag="VKU03_16025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020176273.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbjQ family protein"
                     /protein_id="HLJ72823.1"
                     /translation="MIVSTTNDIAGHKIVRHLGLVRGITVRSRSVVGNFVGGLQSVFG
                     GKLSVYVGLAETARQEAFDHMCEHAAQGGANAVIGMRYDANEIMDGITEVLAYGTAVW
                     VEPV"
     gene            complement(2180..>2746)
                     /locus_tag="VKU03_16030"
     CDS             complement(2180..>2746)
                     /locus_tag="VKU03_16030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020629025.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HLJ72824.1"
                     /translation="FAEATSRDGALGELFRLKEQGLTKAVGLAAGPVTMMIPLLRDWD
                     FDALITHNRFTLANRNADAMLNFAASKGIAVLNAAPYAGGVLAKGSQSYRRYVYQEAS
                     DAMLDPIRRIEEICARHGVPPGAAALQFSMRDPRVASTICGVSKPERVRQTLEWAQFP
                     IPDALWRELQAVRFSTGDPEETREYRLG"
BASE COUNT          479 a          891 c          894 g          482 t
ORIGIN      
        1 cgtttgcgcg taactcggga ggcgctatgg cgggttgacg gcgatcggcc ggcgtggcgc
       61 cgcggcgctg aaaggttacg tctcagacgc cgtgacaacc tcagactttc cccggacagc
      121 cctgcgccgc gcggggcgaa gggattcaca tcttctcgac ctgcccgaac tccagatcca
      181 ccggcgtcgc gcggccgaaa atcgagacgg cgactttgac gcgcgagcgc gcctcgtcga
      241 tttcctcgac cacgccgctg aacgaggcga acggcccgtc ggcgacccgc acctgctcgc
      301 cgatgtcgaa gcggatcgag gatttcggcc gctcgacgcc ttcggcgacc tggcccttga
      361 ttcgctcggc ctcgacgtcg gggataggca tcggcttctt gtcggcgccg agaaagccgg
      421 tgactttcgg cgtgttcttg atcaggtggt agacgtcgtc ggtcaaatcg catttgacca
      481 gcacgtagcc gggaaagaac tttcgctccg aactgacctt gcggccgcgc ctgacctcga
      541 cgacctgctc ggtcggcacc atgatctctt cgaacttctc ggcgaggccg cgctgcgccg
      601 cctgctcttt cagcgattcg gcgaccttct tttcgaaatt cgaataagcg tggacgatgt
      661 accagcgcat gctcatatcg acatccgtag cgaaatgtcc ggagatgtag cgccttggct
      721 tgaaagctgc cgatcagcca tgacggccga ttcccagcgc gaagccgatg gcgaaatgga
      781 tgatttcgtc gaccgtcagg aagaacaggc tcgccaggac caccatcagc aggaccagcc
      841 cggtcgtgat cagcgtctcg cgccggctcg gccagacgac tttcgcgcct tcggcgcgca
      901 cctgctgaat gaattcgaat ggattggtca tgataagggt ccgcgagccg agccgaggcg
      961 acgccttgcg gccggccaga aagatttgcg gcgcgggacc tctcggccgc gcgccacgcc
     1021 tgtctcatat aggaaagcgc cagtccacgc caagggaaat ttgcaacccg gctcatcgtc
     1081 ctggaatgtc aggctttggc cgcgggtgtc gcgcctaatg ggcgccagaa gccccgcccg
     1141 gcggcaaggc cggatggggc cttcagcctc cgcggcgccg ctcaatcgag gacttcgatc
     1201 acccgccggg ttcgcggttc gaccaaaacc ggctcgtcgt tgacgatcgc atagcgatat
     1261 tcggtgacgc cgtattgcgg cggcgccggg taataagcga cgcctggcgg cagcacgtcg
     1321 ccgacgacca cgcgctcggc cgtccatgga gccggcgcgg gatagaccgg cgcggcatag
     1381 accggccccg gcgggacgta gtaggccggc ggggcgaggc caaccgctcc gggcgcgacg
     1441 acaggcggaa caggcgtttc aaccacccat tcctgggcat aggccgtcgc aggcgtcgcg
     1501 gcgattaagg tcgcgagaca ggccgctttc gacatggttt ctctccttgc cgtctgggtt
     1561 gcggccgaaa cgtctctgga atccgatggt tcccgccgcc gcaatggttt gcgcccgcgg
     1621 cgtgagctcg gcgatttcgg gggccggctt tggtcggcgc gcgggcgtct ctcaggcgga
     1681 agtggagatt gatgcgggcc cgtcggcgct taagcgctgg caggagtgga ggggctcgaa
     1741 cccccagccc ccggttttgg agaccggtgc tctaccaatt gagctacact cctagcgcgg
     1801 cgcgttattg cgcaaccggc ctgccgacgc aagacttcgc ggcgatcaga ccggctcgac
     1861 ccagaccgcc gtgccatagg cgaggacttc agtgatcccg tccatgattt cattggcgtc
     1921 gtagcgcatg ccgatcaccg cattggcgcc cccctgagcg gcgtgctcgc acatatggtc
     1981 gaacgcttct tgccgggcgg tctcggcgag accgacatag accgacagct tgccgccgaa
     2041 caccgattgc aagccgccga cgaaattgcc gacgacgctg cgcgagcgca ccgtgatgcc
     2101 gcgcaccaag ccgagatgac gaacgatttt atggccggct atgtcattgg tcgtggagac
     2161 gatcatccga agccctctct tagcctaaac ggtattcgcg agtctcctcg ggatcgccgg
     2221 ttgaaaaccg caccgcttgc agctcccgcc acagcgcgtc cgggatcggg aattgcgccc
     2281 attcaagcgt ctgccggacg cgctccggct tgctgacgcc gcagatggtc gaggccacgc
     2341 gcggatcgcg catcgaaaat tgcagcgccg ccgcgccggg cggaacgccg tggcgggcgc
     2401 aaatctcctc gatgcggcgg atcggatcga gcatggcgtc ggacgcctcc tgatagacat
     2461 agcgccggta ggattgcgag cccttggcca gcacgccgcc ggcataaggc gcggcgttca
     2521 gcaccgcgat tcctttcgac gcggcgaaat tcagcatggc gtcggcgttg cggttggcga
     2581 gcgtgaaccg attgtgggtg atcagcgcgt cgaaatccca atcgcgcaga agcgggatca
     2641 tcattgtcac cgggccggcg gcgaggccaa cggccttggt caggccctgc tccttgaggc
     2701 gaaacagttc gcccaacgcg ccgtcgcggc tcgtcgcctc ggcgaa
//
LOCUS       DATGJW010000528         5504 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_723167, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000528 DATGJW010000000
VERSION     DATGJW010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5504)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5504)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5504
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723167"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..144)
                     /locus_tag="VKU03_16035"
     CDS             complement(<1..144)
                     /locus_tag="VKU03_16035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018642790.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016702 - oxidoreductase activity, acting
                     on single donors with incorporation of molecular oxygen,
                     incorporation of two atoms of oxygen [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carotenoid oxygenase family protein"
                     /protein_id="HLJ72825.1"
                     /translation="MAASAVREMRGNFRPIAIECDAAFLPVRGELPRELNGCLYRNGP
                     NPQF"
     gene            238..945
                     /locus_tag="VKU03_16040"
     CDS             238..945
                     /locus_tag="VKU03_16040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014494046.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HLJ72826.1"
                     /translation="MPRSAKTVRSRSGARAVKRAPGGRQAKSARLYHHGALREALLQA
                     AERLLERDGVQGLTLRAAAREAGVSHAAPKHHFGDLSGLLTDLAIVGFERFRGYLATG
                     LQDDLSPREKLDAIGRGYVAFAKAHPGLFLMMFRSERLDMSRPALRAAARASQDVLAS
                     ALGESGAGGGRLTLEAAGRLARAWSLVHGFSILLIDGRWKPLLARLPEGSTAEDLLEA
                     AIDSASARRSGGQAAPI"
     gene            complement(942..1700)
                     /gene="hutC"
                     /locus_tag="VKU03_16045"
     CDS             complement(942..1700)
                     /gene="hutC"
                     /locus_tag="VKU03_16045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006332388.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine utilization repressor"
                     /protein_id="HLJ72827.1"
                     /translation="MREAIASEGDRSGADTAPSSLHQRILADIEARILSGEWPPGHRI
                     PFEHELTVQYRCSRMTVSKVLTQLAKAGLVARRRKAGTFVTRPHSQAAVLEIHDIKAE
                     AAALGRPYRFEIAARRKRKFARADRQRLDPPVSGEALEITCRHFAGAQPFCLEERLIN
                     LTAVPEAENEMFEDLSPGAWLVQRVPWTAAEHRIRATGADRRMATALELAEGAPCLVI
                     ERRTWRAAEPVTYVKLTYPAEAHELVARFAPSQR"
     gene            complement(1697..3049)
                     /locus_tag="VKU03_16050"
     CDS             complement(1697..3049)
                     /locus_tag="VKU03_16050"
                     /EC_number="3.5.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012654230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formimidoylglutamate deiminase"
                     /protein_id="HLJ72828.1"
                     /translation="MRAVHAKDALTPRGWAGDVRLVLADGTIGAVEIGVPPQPDDERC
                     GALVPGMPNVHSHAFQRGMAGLAETRGPASDNFWTWREAMYRFALAMTPDDVEAVAAM
                     LYVEMIEAGFTRVGEFHYLHHDRDGRPYSAIGEMASRIAAAASQSGVRLTLLPCFYAH
                     STFGGAPPRSEQRRFICDLDQFARILDEARRAASALAGSTIGLAPHSLRAVAPEELSA
                     LLAMAGEAPIHIHAAEQTKEVDDCVAWCGARPIEHLLKIAPVDRRWCLIHATHMTKIE
                     TVRLAASGAVAGLCPVTEANLGDGTFNAPVFRTQGGQFGIGSDSNVLIGVADELRQLE
                     YAQRLAHCERNVMGDARMSTGRALFEAALNGGGAALGAPARLESGAPADFVSLKTDHP
                     ITQHLKPDGVLDAWIFVERNLVDGVWIAGEKLVENGRHKRREAIEKRFRGAVQSLRAA
                     "
     gene            3118..4335
                     /gene="hutI"
                     /locus_tag="VKU03_16055"
     CDS             3118..4335
                     /gene="hutI"
                     /locus_tag="VKU03_16055"
                     /EC_number="3.5.2.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015318309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050480 - imidazolonepropionase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="imidazolonepropionase"
                     /protein_id="HLJ72829.1"
                     /translation="MAAQPAQMRVWRNARLATFAAASPGLGIIEPGALAAQDGRIVYA
                     GPEREAPAALLADAEDCEGRWITPGLIDCHTHLIFAGNRAQEFELRLAGASYEEIARA
                     GGGIVSSVKALRAASEAELVSEALPRLDALIKEGATTVEIKSGYGLDLANEAKALRAA
                     RRLADERAVAIRTTFLGAHALPPEMRDDRRGYIDKVAHEMIPWIARDGLADAVDGFCE
                     GVAFSPDEIARVFAAARAHGLPVKLHADQLSNLRGAALAARFGALSADHLEYADEDGV
                     AAMAAAETVAVMLPCAYYFIRETQRPPIDLFRKHGVKMAVATDCNPGTSPLTSLLLAM
                     NMAATLFRMTVEECLAGATREAARALGLAAETGTLEAGKWADLAIWNIERPAELVYRL
                     GFNPLHARVWRGR"
     gene            4332..>5504
                     /locus_tag="VKU03_16060"
     CDS             4332..>5504
                     /locus_tag="VKU03_16060"
                     /EC_number="4.3.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012331557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine ammonia-lyase"
                     /protein_id="HLJ72830.1"
                     /translation="MSATAIKPGQAALADWRAIYRGGGLALDPSYRENVAASAAAVAR
                     IVAKGEPVYGLNTGFGKLASLRIDAADLARLQRNIVLSHAAGVGAPTPEAIVRLMMAL
                     KLASLAQGASGVRLETFALLEAMLERSVVPVVPSQGSVGASGDLAPLAHMTAAMIGVG
                     EAYLRGQRLPAPQALAAAGLTPIELGPKEGLALLNGTQFSTAHALAALFEAEVLFRSA
                     LATGALSTDAARGSDAPFDPRIHALRRHRGQIEVAEALRALMQKSAIRASHLVGDERV
                     QDPYCLRCQPQVMGAALDVLRQAAATLADEANGVSDNPLIFAEDDAALSGGNFHAEPV
                     AFAADIVALGLCEIGSLAERRIAMLVDSALSGLPAFLTPRPGLNSGFMIAQVAAAAL"
BASE COUNT          838 a         1902 c         1885 g          879 t
ORIGIN      
        1 gaattgcggg ttgggtccgt tgcggtagag gcagccgttg agctcgcgcg gcaattcgcc
       61 gcgaactggg aggaacgcgg cgtcgcattc gatggcgatc gggcggaaat tgccccgcat
      121 ctcccgaacc gcgctcgcag ccatgatcgc gctccaaatc ttgacggtgt aaagatacct
      181 aggcgttgct cgccgccgcg tcaagctcta tcttgacagc gtcaagaagg atgaatcgtg
      241 ccgcgctcag ccaagaccgt ccgcagccgc agcggggccc gcgccgtgaa gcgcgccccg
      301 ggcgggcggc aagcgaaaag cgcccggctc tatcatcacg gcgccttgcg cgaggcgctg
      361 ctgcaagccg ccgagcggct gctcgagcgc gacggcgtgc aaggcctgac gctgcgcgcc
      421 gcggcgcgcg aagccggcgt ctcgcacgcc gcgcccaagc accatttcgg cgacttgtcc
      481 ggccttttga ctgatctcgc cattgtcggc ttcgagcgct tccgcggcta tctggcgacg
      541 gggctgcaag acgatctttc gccgcgcgag aagctcgacg cgatcggccg cggctatgtc
      601 gctttcgcga aggcccatcc cggcttgttc ctgatgatgt tccgcagcga gcggctcgat
      661 atgagccggc cggctctgcg cgccgcggcg cgggcgagcc aggatgtcct ggcgtcggcg
      721 ctcggcgaaa gcggcgcggg cggcggccgg ctgacgctcg aagccgccgg ccggctcgcg
      781 cgggcctggt cgctcgtgca cggcttctcg atcctgctga tcgacggacg atggaagccg
      841 ttattggcgc gcctgccgga gggctcgacc gcggaggatt tgctggaggc ggcgatcgat
      901 agcgcaagcg cgcgccgcag cggcggtcag gctgcgccga tctagcgctg cgagggcgcg
      961 aagcgcgcga ccagttcgtg cgcctccgca gggtacgtga gtttcacata ggtgacgggc
     1021 tcggcggcgc gccacgtgcg gcgctcgatc accagacacg gcgcgccttc ggcgagctcc
     1081 agcgccgtcg ccatccgccg atcggcgccg gtcgcgcgga tgcgatgttc ggcggcagtc
     1141 cagggcacgc gctgcaccag ccaggcgccg ggcgataaat cctcgaacat ctcgttctcc
     1201 gcctcgggga cggcggtcag gttgatcagc cgctcctcga ggcagaacgg ttgcgcgccg
     1261 gcgaagtggc ggcaggtgat ttcgagcgcc tcgcccgaga ccggcggatc cagccgctgc
     1321 cggtcggcgc gcgcaaattt gcgcttgcgt cgggcggcga tctcgaagcg atagggacgg
     1381 ccaagcgccg ccgcctcggc cttgatgtcg tgaatctcga gaacggcggc ctgcgaatgc
     1441 ggccgtgtga cgaacgtgcc ggccttgcgc ctgcgcgcga cgaggccggc cttggcgagc
     1501 tgggtcagga ccttgctcac cgtcatccgc gagcagcgat attggacggt caactcatgc
     1561 tcgaacggaa tgcgatggcc gggcggccac tcgccggaga ggatgcgcgc ttcgatatcg
     1621 gcgaggatgc gctgatgcag cgacgaaggc gccgtgtcgg cgccgctccg atcgccctcg
     1681 ctcgcaatcg cttccctcat gccgcgcgca gggactggac cgcgccgcga aagcgcttct
     1741 cgatcgcctc gcgccgcttg tggcgcccat tctcgacgag cttttcgccg gcgatccaga
     1801 cgccgtcgac gagatttcgc tcgacgaata tccaggcgtc gagcacgccg tcaggcttga
     1861 gatgctgcgt gatcgggtga tcggttttca gcgaaacgaa atccgccggc gcgccggatt
     1921 caagccgcgc cggcgcgccg agcgccgcgc cgccgccatt gagagccgcc tcgaacaggg
     1981 cccggcccgt cgacattctg gcgtcgccca tcacgttgcg ctcgcaatgg gcgagccgct
     2041 gcgcatattc gagctggcgc aattcgtcgg cgacgccgat cagcacgttg gaatcggagc
     2101 cgatcccgaa ctggccgccc tgggtgcgaa agaccggcgc gttgaacgtc ccatcgccga
     2161 gattggcttc cgtgacaggg caaaggccgg cgacggcgcc gctcgccgcc agccgaactg
     2221 tttcgatctt ggtcatatgc gtcgcgtgga tcaggcacca gcgccgatcg accggcgcaa
     2281 tcttgagaag atgctcgatt ggccgggcgc cgcaccaggc gacgcaatcg tcgacctcct
     2341 tcgtctgctc ggcggcgtga atatggatcg gcgcttcgcc ggccatcgcg aggagcgcgg
     2401 aaagctcctc cggcgcgacg gcgcgcagac tatgcggcgc gagcccgatc gtcgagccgg
     2461 ccagcgccga cgccgcccgg cgcgcttcgt cgagaattcg cgcgaattga tcgagatcgc
     2521 agatgaagcg ccgctgctcg gaacgcggcg gcgcgccgcc gaatgtcgaa tgggcgtaga
     2581 agcaaggcag cagggtaagg cgcacaccgc tctggctcgc cgcggcggcg atgcgcgacg
     2641 ccatttcgcc gatcgccgag taaggccggc catcgcgatc gtgatgcaaa tagtgaaact
     2701 cgccgaccct ggtgaagccg gcctcgatca tctcgacata gagcatcgcg gcgaccgcct
     2761 cgacatcatc aggcgtcatg gcgagcgcga aacggtacat cgcctcgcgc caggtccaga
     2821 aattgtccga ggcgggaccg cgcgtctcgg ccaatccggc catgccgcgc tggaaggcgt
     2881 gactgtgcac attgggcatg ccgggaacga gcgctccgca acgctcatcg tccggctgcg
     2941 gcggaacgcc gatctcgacg gcgccaatcg tcccgtccgc caggaccaag cgcacatcgc
     3001 ccgcccagcc ccgcggcgtc agcgcgtctt tcgcgtgcac ggctctcatt ggcgccttgc
     3061 cccctcagga ttatgtctat acattatctc gacggacaga caacaggacc accggccatg
     3121 gcggcgcaac cggctcaaat gcgcgtctgg cgcaatgcgc gcctggcgac tttcgccgcc
     3181 gcctcgcctg ggcttgggat tatcgagccg ggcgcgctcg ccgcgcaaga tggccgcatc
     3241 gtctatgccg gcccggaacg cgaagcgccg gcggcgctcc tggcggacgc cgaagattgc
     3301 gagggccgct ggatcacgcc cggcctgatc gactgccata cccatctgat cttcgccggg
     3361 aatcgcgccc aggaattcga attgcgcctc gccggcgcct cctacgaaga gattgcgcgg
     3421 gccggcggcg gcatcgtttc gtcggtgaaa gcgctgcgcg cggcgagcga agccgagctc
     3481 gtcagcgagg ccctgccccg gcttgacgcg ctgatcaaag agggcgcgac cacggtcgag
     3541 atcaagtccg gctatgggct cgatctcgca aacgaggcga aagcgctgcg cgcggcgcgc
     3601 cggctcgcgg acgagcgcgc ggtcgcgatc cgcacgacct ttcttggcgc tcatgcgctg
     3661 ccgccggaaa tgcgagacga tcggcgcggc tacatcgata aagtcgccca cgagatgatc
     3721 ccttggatcg cccgcgacgg cctcgccgac gcggtcgacg gcttttgcga aggcgtcgcc
     3781 ttctcgccgg acgagatcgc ccgcgtcttt gcggcggcgc gcgctcacgg cctgccggtc
     3841 aagctgcacg ccgaccaatt gtccaatctg cgcggcgccg cgctcgccgc gcgcttcggc
     3901 gcgctgtcgg ccgaccatct cgaatatgcc gacgaggacg gcgtcgccgc catggcggcg
     3961 gcggaaaccg tcgccgtgat gctgccgtgc gcctattatt tcatccgcga gacgcagcgc
     4021 ccgccgatcg atctctttcg caagcacggc gtcaaaatgg ctgtcgcgac cgattgcaat
     4081 cccggcacct cgccgctgac ctcgcttctg ctggcgatga acatggcggc gactctgttc
     4141 cggatgacgg tcgaggaatg cctcgccggc gccacccgcg aggcggcgcg cgcgcttggc
     4201 cttgccgccg agacgggaac gctcgaagcc ggcaaatggg cggacttggc gatctggaat
     4261 atcgagcggc cggccgagct cgtctaccgc ctcggcttca atccgctgca cgcccgcgta
     4321 tggagaggcc gatgagcgcg acggcgatca agcccggcca ggctgcgctc gccgattggc
     4381 gggcgatcta tcgcggcggc gggctggcgc tcgatccgtc gtaccgcgag aacgtcgccg
     4441 ccagcgccgc cgcggtggcg cggatcgtcg ccaagggcga gccggtctat gggctcaata
     4501 ccggcttcgg caagctcgcc agcctcagga tcgacgccgc cgacctcgcc cggctgcagc
     4561 ggaacatcgt cctatcgcac gccgcgggcg tcggcgcgcc gacgccggaa gcgatcgtgc
     4621 ggctgatgat ggcgctgaag cttgcttcct tggcgcaggg cgcctcgggg gtgcgtctcg
     4681 agaccttcgc cctgttggag gcgatgctcg agcgttcggt cgttccggtc gtgccgagcc
     4741 aaggctcggt cggcgcttcc ggcgatctcg ccccgctcgc ccatatgacc gccgcgatga
     4801 tcggcgtcgg cgaagcctat ctgcggggcc agcgcctgcc cgcgccgcag gcgctcgccg
     4861 ccgccggcct gacgccgatc gagcttgggc cgaaagaggg tttggcgctg ctcaacggca
     4921 cccaattctc gaccgcccac gccttggcgg cgttgttcga ggcggaagtc cttttccgct
     4981 cggccttggc gaccggcgcg ctttcgaccg acgcggcgcg cggctccgac gcgccgttcg
     5041 atccgcgcat ccacgccttg cgcaggcatc gcggccagat cgaagtcgcc gaggcgttac
     5101 gcgccttgat gcagaaaagc gccatccgcg cttcgcatct tgtcggcgac gagcgcgtgc
     5161 aggatcctta ttgcctgcgc tgccagccgc aagtgatggg cgcggcgctt gatgtgctgc
     5221 ggcaagcggc ggcgaccttg gcggacgagg cgaacggcgt ctccgacaac ccgctgattt
     5281 tcgcggagga cgacgcggct ttgtcgggcg gcaatttcca cgccgagccc gtcgccttcg
     5341 cggccgacat cgtcgccctg gggctgtgcg agatcggctc gctcgccgaa aggcgaatcg
     5401 ccatgctggt cgattccgca ctctcgggcc tccccgcctt cttgaccccc cggccgggcc
     5461 tcaattcagg cttcatgatc gcccaggtcg ccgcggcggc gctg
//
LOCUS       DATGJW010000529         6990 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1140948, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000529 DATGJW010000000
VERSION     DATGJW010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6990)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6990)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6990
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1140948"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..320
                     /locus_tag="VKU03_16065"
     CDS             <1..320
                     /locus_tag="VKU03_16065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GPW/gp25 family protein"
                     /protein_id="HLJ72831.1"
                     /translation="AQDLSAAPDVRSSILNYGLPDLSWRTLDENGLSDVAREIQTALA
                     DFEPRLDRNSIRARRDEAAREEELKLRFLVKADLVARPVNVPLEFVAEIELDSGKIKI
                     DRL"
     gene            326..2284
                     /gene="tssF"
                     /locus_tag="VKU03_16070"
     CDS             326..2284
                     /gene="tssF"
                     /locus_tag="VKU03_16070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318181.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssF"
                     /protein_id="HLJ72832.1"
                     /translation="MNREFLEFYNRELALLREQAAEFAQEYPGVAERLGGLIGERIDP
                     MIGGLLEGSAFLAARVQLKLKHEFSEFTTNLIDQLVPHYLAPTPSAMLAQVRPTFGDP
                     ALLDGKTIPRGAYLDAAYRELERDVACRFALAAPIQLWPFEIAAAEYFSAPAPLQALG
                     VPVGPECLAGLRLQLRMRAVPRVEDEPPDSAAEARPELRFSSCRIKNLALHFLGHEAD
                     AVLLYEQMFAHCRGVYFRYLDSFGDPVARAGGDAMLRQIGFGEDEALIFNDKRVFVGF
                     DYLREYFTFPRRFLGVELAGLDKIAPGLPAKTVDVVFAFDDVNGRLAAAINKDFFALY
                     AAPAVNLFEKTLDRTTVKSNQHEYHLVPDRGRALDYEVNRVVNVFAHIPGRPQKVAVE
                     PLYTAAAGRSATGLYYTLRRLPRRRSAQERRYGQQSDYTGTETFLSLGQRAGAEEPEQ
                     VAELSVEALCTNRHLTEHLPVGEGGADFRFLDDTSLDVRCVAGPTRPLEPIMTTMTGR
                     SADTSGGDIAWRAINMLSLNHLGLVQRAGGDSARSLKEMLALFGDMSDSATERKIRGI
                     RAVDSRPVVRRVQRAGAVAAARGTEITVTIDDKAYEGSGVFLMGAALDRFFCEYAGVN
                     HFTQTVIRTAERGEIMRWPPRIGRRGDL"
     gene            2281..3321
                     /gene="tssG"
                     /locus_tag="VKU03_16075"
     CDS             2281..3321
                     /gene="tssG"
                     /locus_tag="VKU03_16075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014493486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system baseplate subunit TssG"
                     /protein_id="HLJ72833.1"
                     /translation="MKAEERLLAEPWRFDLLDTLRRYEREHPERPRIGDAETLADEYV
                     VVTQNPYLEFPASTLEAAEREGNGRMRLVARFLGMFGPQGALPLTTTEEAYHWLLDRD
                     DAFPRFADIFQRRFLSLFFRAWADARPIAQQDRPDKDRFVAFVGSAIGVGAPSYRGAD
                     SLSDFAKMEFAGLIAPKAKSASRLRRMIAGLFDVKVEIDEFVGSWLALDRAERSRLGM
                     AQSALGRDLLVGAAVFSVSDKFRVRIFVKDFDQYLRFLPDGDLARGIADAVFLQLGDE
                     FDWDMELAIPAGEVKPVRLGQGARLGWTGWTAPNWSDTDRTIRADARFHVVSRLNRGG
                     GKAAGGADGRGH"
     gene            3305..5947
                     /gene="tssH"
                     /locus_tag="VKU03_16080"
     CDS             3305..5947
                     /gene="tssH"
                     /locus_tag="VKU03_16080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018318183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0033104 - type VI protein secretion
                     system complex [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0033103 - protein secretion by the type VI
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type VI secretion system ATPase TssH"
                     /protein_id="HLJ72834.1"
                     /translation="MGADISVEQVAGKLNRVGYDTFIQALRHAKSAGNRNVELAHWLA
                     HILQQERSDITQTADHFKLDRAKMLMDLGDAIGALRQNQTEMPEISDLVQELLDNGWY
                     YATLFFGETQIRTGHLLVGAFKRNELRRALTGLSKEFGKINVDTLAAEHRAIWRNSDE
                     ENLRPIDGSGIAAAGTEGAAAAGARGSTALDRFSQDLTARAQSGEMDPILGRDEEIRQ
                     IVDVLMRRRQNNPILTGEAGVGKTAVVEGFAQRIAAGDVPPPLRGVRLCALDVGLMQA
                     GASMKGEFEQRLRSVIDEVQSSPTPIILFIDEAHTLIGAGGAAGTGDAANLLKPALAR
                     GTLRTIAATTWAEYRQYFEKDPALTRRFQPIQIGEPDVARAATMLRGLVAPMEKHHKV
                     RVSDAAIVAAVQFSHRYIPARQLPDKAVSLLDTACARVAISQSAKPAAIEDAQVSIAA
                     LRQEKEALARERDLGEANEERLGEIETALKSQGESLAKLEEEWASEGKLVEEIQALRA
                     KLAEEKKEASEESVERAQLREKLAAIEAINPERRMIYPHVDEQAVASVVSDWTGIPVG
                     RMVADEVQTILNLADILNRRVVGQAHGLKMIAKRIETNRAKLDNPNKPIGVFMLCGPS
                     GVGKTETALALAETLYGGEQNMITINMSEFQEAHTVSTLKGAPPGYVGYGEGGRLTEA
                     VRRRPYSVILLDEVEKAHPDVHEIFFQVFDKGVMEDGTGRRIDFKNTLIILTSNVGTD
                     AIMQAAKGPLADDPEKLAAALRPDLLNVFPPALLGRIVTIPYYPLSPEMLGGIVRLQL
                     DRIGKRIEDNHGAKFVYDQAVVDHIVSLCNDPDSGGRVIDNVITNTILPSLSREILSR
                     SLARETISQAKTTIADGGFAYEVT"
     gene            6080..6370
                     /locus_tag="VKU03_16085"
     CDS             6080..6370
                     /locus_tag="VKU03_16085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018272191.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72835.1"
                     /translation="MANRFEQVDEVQPDAISIVLEQRGDGQWAKVHCPMSASPALSAD
                     KMSESLPPKDALSGAVKLANHLKLAIVVVDPDSIWKREWGELYRWSPDDEAS"
     gene            6367..6792
                     /locus_tag="VKU03_16090"
     CDS             6367..6792
                     /locus_tag="VKU03_16090"
                     /inference="COORDINATES: protein motif:HMM:NF018037.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1036 domain-containing protein"
                     /protein_id="HLJ72836.1"
                     /translation="MKARLLRKSGVPIALLTLLAAAAPAKADLHVCNRTKYLLNIAVG
                     YVDGEDFATEGWWSVTPNSCATPIKRPLAGRYVYLYATDVDAADVLQGTVSMCIGRRK
                     FKISGISDCWRRGLQAVNFQEIDTLNSSDWTVFLNDAAQ"
BASE COUNT         1240 a         2323 c         2255 g         1172 t
ORIGIN      
        1 cggcccagga cctctcggcc gcgccggacg tgcgcagctc gatcctcaat tacggcctgc
       61 ctgacttgag ctggcgcacg ctcgacgaga acgggctcag cgacgtcgcg cgcgaaatcc
      121 aaaccgctct ggcggatttc gagccgcgcc tcgaccgcaa ctcgatccgc gcacgccgcg
      181 acgaggcggc gcgcgaggag gagctgaagc tgcgcttcct ggtcaaggcc gatctcgtcg
      241 cccggccggt gaacgtcccg ctggaattcg tcgccgagat cgagctcgat tccggcaaga
      301 tcaagatcga ccgtctttag gggccatgaa ccgggaattc ctcgaattct acaaccgcga
      361 gctcgcgctt ctgcgcgagc aggccgccga attcgcgcag gaatatcccg gcgtcgccga
      421 gcggctcggc ggcctgatcg gcgagcgcat cgatccgatg atcggcggcc tgctcgaagg
      481 ctcggccttc ctcgccgcgc gcgtgcaatt gaagctgaag cacgaatttt ccgaattcac
      541 gaccaatctc atcgaccagc ttgtcccgca ttatctcgcg ccgacgccct cggcgatgct
      601 cgcgcaagtg cgtccgacat tcggcgatcc ggcgttgctc gacggcaaga cgattccgcg
      661 cggcgcctat ctcgacgccg cctatcgcga gctggagcgc gacgtcgcct gccggttcgc
      721 ccttgccgcg ccgatccagc tttggccgtt cgaaatcgcc gcggctgaat atttttcagc
      781 gccggcgccg ctccaggcgc tcggcgtgcc ggtcggcccg gaatgcctcg ccggcttgcg
      841 cctgcagctc cgcatgcgcg ccgttccgcg cgtcgaggac gagccgcccg attccgccgc
      901 cgaggcgcgg ccggagctca ggttttcctc gtgccggatc aagaaccttg ccctgcattt
      961 cctcggccac gaagcggacg cggtgctgct ctacgagcaa atgttcgccc attgccgcgg
     1021 cgtctatttt cgctatctcg atagcttcgg cgatccggtg gcgcgggccg gcggcgacgc
     1081 catgctccgc cagatcggct tcggcgagga cgaggcgctg atcttcaacg acaagcgcgt
     1141 cttcgtcggc ttcgactatt tgcgcgaata tttcaccttc ccgcgccgct tcctcggcgt
     1201 cgagcttgcc ggcctcgaca agatcgcgcc cggcctgccg gccaagacgg tcgacgtggt
     1261 cttcgccttc gacgatgtga acggccgcct cgccgccgcg atcaacaagg actttttcgc
     1321 gctttacgcc gcgccggcgg tcaatctctt cgagaagacg ctggaccgca cgacggtcaa
     1381 gtccaaccag cacgaatatc atctcgttcc cgatcgcggc cgcgccctcg attacgaagt
     1441 caatcgcgtc gtcaacgtct tcgcccacat tccggggcgg ccgcagaaag tcgcggtgga
     1501 gccgctctat accgctgcgg cgggccgcag cgcgacgggc ctctattata cgttgcgccg
     1561 gttgccgcgg cgccgatcgg cgcaggagcg ccgctacggc cagcagtcgg actataccgg
     1621 caccgaaacg tttctctctc tcggtcagcg cgccggcgcg gaagagccgg agcaggtcgc
     1681 cgagctcagc gtcgaagcgc tctgcaccaa ccgtcacctc accgagcact tgccggtcgg
     1741 cgaaggcggc gccgatttcc gctttctcga cgacaccagc ctcgacgtcc gctgcgtcgc
     1801 cggtccgacc cggcccttgg agccgatcat gacgacgatg accgggcgaa gcgccgacac
     1861 gtccggcggc gacatcgcct ggcgcgccat caacatgctg agcctcaatc atctcggcct
     1921 cgtccagcgc gccggcggcg acagcgcccg ctcgctgaaa gaaatgctcg ctctgttcgg
     1981 cgacatgtcg gacagcgcca ccgagcgcaa aatccgcggc attcgcgctg tcgattcgcg
     2041 gccggtcgtg cgccgggtgc agcgcgccgg cgcggtcgcg gcggcgcgcg gcacggaaat
     2101 cacggtgacg atcgacgaca aagcttatga aggctcgggc gtgttcctga tgggcgcggc
     2161 gctcgaccgg ttcttttgcg aatatgccgg cgtgaaccac ttcacccaga ccgtcatccg
     2221 caccgccgag cgcggcgaga tcatgcgctg gccgccgcgc atcggcagaa ggggcgacct
     2281 gtgaaggcgg aagagcgcct tctcgccgag ccttggcggt tcgacttgct cgacacgctg
     2341 cgccgttacg agcgcgagca tcccgagcgg ccgcgcatcg gcgacgccga gactctcgcc
     2401 gacgaatatg tcgtcgtcac ccagaatccg tacctggagt tccccgcctc cacgctcgag
     2461 gccgccgagc gcgagggcaa cgggcgcatg cgcctcgtcg cccgcttcct cggcatgttc
     2521 ggtccgcaag gcgctctgcc gctgaccacg accgaagagg cctatcactg gctgctcgat
     2581 cgcgacgacg cctttccgcg cttcgccgac attttccagc gccgttttct ctccctgttc
     2641 ttccgcgcct gggccgacgc gcggccgatc gcgcagcagg accggccgga caaggaccgt
     2701 ttcgtcgcct ttgtcggctc ggcgatcggc gtcggcgcgc catcttatcg cggcgccgat
     2761 tccctgtccg atttcgccaa gatggagttt gccgggctga tcgcgcccaa ggcgaaaagc
     2821 gcttcgcgcc tgcgccggat gatcgccggc ctgttcgatg tcaaggtcga gatcgacgaa
     2881 ttcgtcggct cgtggctggc gctggacagg gccgagcgct cgcggctcgg catggcgcaa
     2941 agcgcgctcg gccgcgatct ccttgtcggc gccgcggtct tcagcgtctc ggacaagttc
     3001 cgcgtccgca tcttcgtcaa ggatttcgat cagtacctgc gtttccttcc cgacggcgat
     3061 ctcgccaggg gaatcgccga cgcggtcttt ctgcagctcg gcgacgaatt cgattgggac
     3121 atggagctcg ccattcccgc cggcgaggtc aagccggtgc ggctcggcca gggcgcgcgg
     3181 ctcggctgga ccggatggac ggcgccgaat tggtcggaca cggatcgcac gatccgcgcc
     3241 gatgcgcggt ttcatgtcgt gagccgcctg aaccgcggcg gcggaaaagc ggcgggaggt
     3301 gccgatgggc gcggacatta gcgtcgagca ggttgccgga aagctcaacc gggtcggcta
     3361 cgacaccttc attcaagcgc tgcgccacgc caaaagcgcc ggcaaccgca atgtcgagct
     3421 ggcgcattgg ctggcccata tcctccagca ggagcgcagc gacatcacgc agacggcgga
     3481 ccatttcaag ctcgatcggg ccaagatgct gatggacctc ggcgacgcga tcggcgcgct
     3541 gcgccagaac cagaccgaaa tgccggaaat ctccgacctg gtgcaggagc tgctcgacaa
     3601 tggctggtac tatgcgacgc tgttcttcgg cgagacacaa atccgcaccg gccatctcct
     3661 cgtcggcgcc ttcaagcgca acgagctgcg gcgcgcgctg accggactgt cgaaagagtt
     3721 cggcaagatc aatgtcgata cgctcgccgc cgagcatcgc gccatctggc gcaattccga
     3781 cgaagagaat ctacggccga tcgacggatc cggcatcgcc gcggcgggaa cggaaggcgc
     3841 ggcggccgcg ggggcgcgag gctcgaccgc gctcgaccgc ttcagccagg atttgaccgc
     3901 gcgggcgcag tcgggagaga tggacccgat cctcggccgc gacgaggaaa tccggcagat
     3961 cgtcgacgtg ctgatgcgtc ggcggcagaa caatccgatt ctcaccggcg aagcgggcgt
     4021 cggcaagacc gcggtagtcg agggcttcgc gcagcggatc gccgccggcg acgtgccgcc
     4081 gcctctgcgc ggcgtcaggc tctgcgctct cgatgtcggc ttgatgcagg cgggcgcctc
     4141 gatgaagggc gagttcgagc agcgcctgcg ctcggtgatc gacgaggtgc aatcctcccc
     4201 gacgccgatc atcctgttta tcgacgaagc ccatacgctg atcggcgccg gcggcgcggc
     4261 cggcacgggc gacgccgcca atctcctgaa gcccgctctg gcgcgcggaa ccttgcgtac
     4321 gatcgctgcg acgacctggg cggagtaccg ccaatatttt gaaaaggacc cggctttgac
     4381 gcgtcggttc cagccgatcc agatcggcga gccggacgtg gcgcgcgccg ccaccatgct
     4441 gcgcggcctg gtcgcgccga tggagaagca ccacaaggtg cgcgtctccg acgcggccat
     4501 cgtcgccgcc gtgcaattct cgcaccgcta tattccggcg cgccagcttc ccgacaaggc
     4561 ggtgagcctg ctcgacacgg cttgcgcgcg ggtggcgatc agccaatcgg ccaagcccgc
     4621 cgcgatcgag gacgcgcagg tgagcatcgc cgccctgcgg caggagaagg aggccttggc
     4681 gcgcgagcgc gaccttggcg aggcgaacga ggagcgcctc ggcgagatcg aaacggcgct
     4741 gaagagccaa ggcgagagct tggccaagct cgaagaggaa tgggcgagcg agggcaagct
     4801 cgtcgaagag attcaggcgc tgcgcgcgaa actcgccgaa gagaagaagg aggcgagcga
     4861 agaaagcgtc gagcgcgcgc agcttcgcga aaagctcgcc gcgatcgagg cgatcaatcc
     4921 agaaaggcgg atgatctatc cgcatgtcga cgagcaggcg gtcgcctcgg tcgtctccga
     4981 ttggacgggc attcccgtcg gccgcatggt cgccgacgag gtgcagacca tcttgaactt
     5041 ggcggacatc ctcaaccgcc gcgtcgtcgg ccaggcgcat ggcctcaaga tgatcgctaa
     5101 gcggatcgag accaaccgcg ccaagctcga caatccgaat aagccgatcg gcgtgttcat
     5161 gctgtgcggc ccgtccggcg tcggcaagac cgagaccgct ctagcgctcg ccgagacgct
     5221 ctatggcggc gagcagaaca tgatcacgat caacatgtcg gaattccagg aggcgcatac
     5281 cgtctcgacg ctgaaaggcg cgccgcccgg ctatgtcggc tatggcgaag gcggaaggct
     5341 caccgaggcg gtgcggcggc ggccctatag cgtcatcctg ctcgatgagg tcgagaaggc
     5401 gcatcccgac gtgcatgaga tcttcttcca ggttttcgac aagggcgtga tggaggacgg
     5461 caccggtcgg cgcatcgact tcaagaacac gctgatcatc ctgacatcga atgtcggcac
     5521 agacgcgatc atgcaggcgg cgaaaggccc gctggccgac gatccggaaa agctcgccgc
     5581 cgcgctgcgg ccggacctgc tcaacgtctt tccgccggct ctgctcggcc gcatcgtgac
     5641 gatcccctat tacccgctgt cgccggaaat gcttggcggc atcgtcaggc tgcagctcga
     5701 ccggatcggc aagcgcatcg aggacaatca cggcgcgaaa ttcgtctacg accaggcggt
     5761 ggtcgaccat atcgtgtcgc tgtgcaacga ccctgattcc ggcgggcggg taatcgacaa
     5821 cgtcatcacc aacacgatcc tgccttcgct gtcgcgcgaa attctgagcc ggtcgctggc
     5881 gcgggagacg atcagccagg cgaagacgac gatcgccgac ggcggcttcg cctatgaggt
     5941 gacgtgagcc ggcgagccgt cattgcgaag agcgaagcga ccaagcaatc cattcgccgg
     6001 gcgacgcccc aagaatggat tgcttggctc gctatgacgg agtggctccg tttaaagtat
     6061 gaaccctggg gaggacgcca tggccaatcg gttcgaacag gtcgacgaag tccagccgga
     6121 cgcgatcagc atcgtgctcg aacagcgcgg cgacggccaa tgggccaagg tgcactgccc
     6181 gatgagcgcg tcgccggcgt tgagcgccga taagatgagc gagagtctgc cgccgaagga
     6241 tgcgctgtcc ggcgcggtca aactcgccaa tcacttgaag ctggcgatcg tcgtcgtcga
     6301 tcccgattca atctggaaac gcgaatgggg cgagctttac cgctggagcc ccgacgacga
     6361 ggcgtcgtga aggcgcggct tttgcgtaag agcggcgttc cgattgcgct tctgacgctg
     6421 ctcgccgcgg cggcgccggc gaaagccgat ttgcacgtct gcaaccggac gaagtatctc
     6481 ctcaacatcg cggtcggcta tgtcgatggc gaggacttcg ccaccgaagg atggtggtcg
     6541 gtcacgccca attcctgcgc cacgccgatc aagcggccgc tcgccggacg ctatgtctat
     6601 ctctacgcca ccgatgtcga cgccgccgac gtcctgcaag ggacggtctc gatgtgcatc
     6661 ggccggcgca aattcaagat ctccggcatt tccgattgct ggcggcgcgg ccttcaggcg
     6721 gtaaacttcc aggaaatcga tacgctgaac tcgtccgact ggacggtttt cctgaacgac
     6781 gcggcgcaat agccgccgcg tccattgcga gccccgctgc gcgcgatgac gaccagcggc
     6841 cgccggagga tttctcattt ccaggcacaa aattctttct gaatatcaga atagatgtta
     6901 ctctgctccg gcgtccattt cttcaaagcc ctcgggagag gaaagaaagt gccggctaac
     6961 tccttgatat gacaatttcc gcggaaatcg
//
LOCUS       DATGJW010000530        14261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1551791, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000530 DATGJW010000000
VERSION     DATGJW010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 14261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 14261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..14261
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1551791"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(198..1187)
                     /locus_tag="VKU03_16095"
     CDS             complement(198..1187)
                     /locus_tag="VKU03_16095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015340248.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2OG-Fe(II) oxygenase family protein"
                     /protein_id="HLJ72837.1"
                     /translation="MSSFPIFDLGAFEKGGAERRRALGRQVDEICRSTGFLAIANHGV
                     ADDVIDGVWSKAKAFFDLPFAEKDKAKAPYKGYPYGYLGPELEALAKSRNVDTPPDLK
                     ESFNGGPASAPAGMTDKEALAFCYAPTIWPQKPAGFVAAWRAYYAAMEDLAARIMKVF
                     AAALNLPEDYFARFIDAPISALRALNYPEQRVPPKPGQLRAGAHTDYGSLTILLPQEG
                     SKGLEIFSNGAWIPVPPIPGAFVINLGDLMQRWTNDRWVSTLHRVVNPPPEEGGLARR
                     QSLAFFHQPNWFAEIACLEACLAPGETPKYEPVFSGPYLMSKFRATVTPNAAA"
     gene            1296..3332
                     /locus_tag="VKU03_16100"
     CDS             1296..3332
                     /locus_tag="VKU03_16100"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012113855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HLJ72838.1"
                     /translation="MPMLPAANLLADATSPYLLQHAHNPVHWRQWGPAALEEARERDR
                     PVLISIGYAACHWCHVMAHESFENEEIAALMNRLFVNVKVDREERPDIDHIYMTALNA
                     LGEQGGWPLTMFLAPDGAPLFGGTYWPPAPRWGRPSFRQILLAVDKAWGEKREALLAQ
                     GANLKAFFAELSQAKGGGAVGPADLSAIAAAYLRQVDPVHGGIGGAPKFPNAPIFRFL
                     WSEAFRDNNQAAKAATRRLLVSLCQGGIYDHLGGGFARYSVDAEWHVPHFEKMLYDNA
                     QIFELLALAHADEPDALFAERAEETFGWLMREMRVGDGERFAFAASQDADQAGEEGSF
                     YTWTAEEIDAALGAESAAFKAAYDVRPEGNWEHRNVLRRTLAAHGDAAAEARLAQARR
                     RLFALREGRPRPALDDKILADWNGLMIAALARAAPFSFAPACLDAAQRAFAFVDENLR
                     DKKGRLVHAWRGRAGAAGLLDDYASMSRAALALFEATGERSYLRQAVALIEEARDLFG
                     EADGGFFITARDAADVPAARPRHAHDGATPSGIGLIAEVLARLHHLTGEKSWRDDCER
                     LIAAFAGPREALAQSPLLLAAADFLERAAVVVVGGDPGDPRAKALAAIALASPDPATC
                     LLRTRDGADWPEGSPGYGRAPIGGAPAAYLCRGAVCSLPVAAPEELRRLTANRS"
     gene            3488..4618
                     /locus_tag="VKU03_16105"
     CDS             3488..4618
                     /locus_tag="VKU03_16105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013530863.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HLJ72839.1"
                     /translation="MKHLGRVSGLALAGLLLSSVAVFAGPKTVSGPGADPECFKPWSN
                     TTKYFQWPKKQGPYRIAVANGFVGNTWRIQMIKTAKAFAELPEIKKDIKEFKVVSTGT
                     DVAAQLGAMEDFINQGYDAIIANVVSPDGFDRIIRLADKNNVVVVPFDNVLDTDKVMQ
                     INEDQKEMGRMSAEWLLKETGKTSGDILEVRGLPGNSVDRDRHVGFREVMEKAPNKFN
                     ITEVVGNWDTGTSQKVTADALAVHGHFDGVFTQGGSDGTVQAMMDAHHPFVPMSGEGE
                     NEYRKQIAEHWKQGLKGMSYGQSPALVAIAMKAAISALKGNVMPQLISIPIPVATYKD
                     LKPGVNYWPDLNVGFFADNQFKPCGVDFTAPQIMAQSEKNTQ"
     gene            4715..6265
                     /locus_tag="VKU03_16110"
     CDS             4715..6265
                     /locus_tag="VKU03_16110"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018183255.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ72840.1"
                     /translation="MKTATAGEYLVLSGVSKRYGGVRALENVDFSCQRGRIHAVLGEN
                     GAGKSTLIKIIAGVVQPDSGELRLDGAVRQFSYPAAATAAGIVCIFQELSLMPDLSVA
                     DNISIASPPTRFGVIDARAQRRRAEALLAEVGCEDVNPLLRVRDLPLSRRQMVEIAKA
                     LGQKPKLLILDEATSALTSADVEKVYALLARLKAENVAILYISHRMHEVEALADRASV
                     FRNGRHIETFDKGARSTAEIVQLMIGRDIATQYPPKPKRPRPAPALKIENLGWEKRLE
                     GISLDVGAGEIVGLGGLDGQGQSTLLLALFGVLRGVTGSIRVNGKAVRPGSPAAAKAA
                     DVGIALVPEDRKTEGLMLPMSIADNLAIASLDALSSGPFIDAAKERKAVDAGIARLQI
                     KIGAKTDAVSTLSGGNQQKVVIAKWLMTGPKIILLNDPTRGIDVGTKQELYRLMRELA
                     DQGAAILFYSTDYDELIGCCDRVAIMYDGRIVRELAGDDLTETNIVESALNIDPAAVR
                     TKQESARV"
     gene            6258..7412
                     /locus_tag="VKU03_16115"
     CDS             6258..7412
                     /locus_tag="VKU03_16115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018459504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72841.1"
                     /translation="MSSVASPTSRSERATQSDLVIRLRQNAGLITAVALFCLVYLFYH
                     LVHPKGFSSAVLVQNADEVFTLATLAMAQTVPVLAAGLDLSVGALMTLVDCLASYLLT
                     YSDKAQVFNIDIGALHLPVLLLPGGAAGVWLGAIICLLTGLLGGFINGCVVVYGRIQP
                     IIATLATGAIYMGLALYLRPTPGGKIGDDLNWAMTNTLGDFASTVHIFDDGAAGWFAP
                     IAGIPVPAILLLLIAAVVWAPFRRTVTGRTVYAIGSAEGAAYMSGLAIQRARLAAFTL
                     GGFFAGCGGLFLAIQTSSGNADIPQAGEYTLNSIAAVVIGGTSLLGGSGGAIGSLFGA
                     LILRVISFCFRIFDVPPLLQPLFEGIVLLVAVSLGALRVLRVKNTLELFR"
     gene            7409..8368
                     /locus_tag="VKU03_16120"
     CDS             7409..8368
                     /locus_tag="VKU03_16120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007596117.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72842.1"
                     /translation="MTTGSLTLSRDDRPIAIAALFIVAILIAGTAYTLERFGSAPLLS
                     PNYLLQQLQIGAFLGIVSAGMMIVILLAHIDLSVPWTLTAAAMMATAVSGPWALPVGL
                     GVGLAVGTVNGLGIAYLRVPSMIFTLGVNAVMRGLMVAYTGGFAPDTHATDLMRTLAA
                     ERTFGMPNALFVWLAVSAIVVIILQRTALGRYVYAIGNREAAAYLAGVNTRHVIVICF
                     ALCGLASGLAGVLLAGYSQKAYQGMGDPYLLPSIAAVVIGGTNILGGRGRYLGTLVGV
                     ILIVLLNSVLSIMEMPEAGRQVIYGGVIILMLLVYGRGERVTS"
     gene            8500..8685
                     /locus_tag="VKU03_16125"
     CDS             8500..8685
                     /locus_tag="VKU03_16125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014889816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72843.1"
                     /translation="MAKASKKTKRGNKAERAAGQAKVIGVTKDGVRILKPRGKATHFT
                     QKEIREAVAAARTARRA"
     gene            8685..9317
                     /locus_tag="VKU03_16130"
     CDS             8685..9317
                     /locus_tag="VKU03_16130"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020179284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72844.1"
                     /translation="MGARDVFINCPFSADYQEFFEAIVYAVIRSGFTPRCARENDDGG
                     EVRIDKICRIIGESRYGIHDISKTELDPASGLPRFNMPLELGLFLGARKFGGRGNANK
                     KALILDRESHRYQAFISDIAGQDIHSHNGNVRRAIECVATWLRDEAGDPDVPGGRAIA
                     TEFERFRGGLPSIAESKRLEPDELTFRDLTAIAAEWILAESGSNGSSSPL"
     gene            complement(9386..10303)
                     /gene="gcvA"
                     /locus_tag="VKU03_16135"
     CDS             complement(9386..10303)
                     /gene="gcvA"
                     /locus_tag="VKU03_16135"
                     /inference="COORDINATES: protein motif:HMM:NF008352.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional regulator GcvA"
                     /protein_id="HLJ72845.1"
                     /translation="MKNPSRLPSLNALRAFEAVSRRLSYARAADELFVTKAAVAQQVR
                     QLETEIGAILVERAGRGLRLTEAGQAGMRDLAEGFEFLARATRAMREASGRRFLVINS
                     SPSFAATWLVGRIGRFKAAHPDIDVLLDANPLEEPLDHPGVDALIRWGAGEFPGFATT
                     LLFEENVFPVCAPRLIEGANPLRAPEDLKSHTLLHLDWNPSFSTWPTWADWLKAAGAA
                     EVDASRGVWFNHMSMALYAAAQGQGVTLTTLAIAADELAAGRVVAPFTTEVHTPYGYY
                     FLCRPEQANSPRISALRDFLAAEAMKSAG"
     gene            10411..10611
                     /locus_tag="VKU03_16140"
     CDS             10411..10611
                     /locus_tag="VKU03_16140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72846.1"
                     /translation="MIHSRHPNENPIAGFGALGRSVCKQILNRLAEASNRAVVRSRFA
                     DLQLRDLEDIGLTIAERDAMLR"
     gene            complement(10668..11540)
                     /gene="copD"
                     /locus_tag="VKU03_16145"
     CDS             complement(10668..11540)
                     /gene="copD"
                     /locus_tag="VKU03_16145"
                     /inference="COORDINATES: protein motif:HMM:NF033808.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0006825 - copper ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper homeostasis membrane protein CopD"
                     /protein_id="HLJ72847.1"
                     /translation="MNLFLIVVRFVHFGSAMALFGASVFLAALAPPLLRAQVAPPLKR
                     IIVVASVAAAVSAALWLLLEAGLMTGDWRAALQTDALAAVLEETGFGHVWRARLALAL
                     LLLVLAFWRGVPPAIIAALSGLFLASLGLVGHAAMLEHAAGLFERANQALHLLAGGFW
                     LGALPPLLICLRRLGEPGFEAQAGVALRRFSGLGHVAVAAVLATGIANTFMILGRPPT
                     NFASPYQASLAMKILTVAAMILLALYNRYQLAPRLALKPEARLALARNTAIEIGLGGV
                     VLALVSVFATFDPA"
     gene            11505..11909
                     /locus_tag="VKU03_16150"
     CDS             11505..11909
                     /locus_tag="VKU03_16150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72848.1"
                     /translation="MDETDDDQEKVQKIAPYPIVPSRKTASARCSVWRSTMGESLAVV
                     AISDDTIDAPFDDLKPGKQSRLQCDAPSQPPVMPGLAPSIRARPQPQTLESVCSGAAW
                     MAGTNPAMTHSDCAGRQRSRRPRVPSFPTALS"
     gene            complement(11947..12324)
                     /locus_tag="VKU03_16155"
     CDS             complement(11947..12324)
                     /locus_tag="VKU03_16155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72849.1"
                     /translation="MTRLLPQVVDAPHDAFSFWSSQNADDHLFGSIAALSYPQPSAPR
                     GASIVLATPTKSGECEAAGVQVVPTARSCGAIQAALMNSGRAVANLEGLPLIENPAGV
                     RYLLAPSAGNGCIVIAVTVLRPK"
     gene            complement(12756..>14261)
                     /locus_tag="VKU03_16160"
     CDS             complement(12756..>14261)
                     /locus_tag="VKU03_16160"
                     /inference="COORDINATES: protein motif:HMM:NF015831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YadA-like family protein"
                     /protein_id="HLJ72850.1"
                     /translation="LAVGNGSTAPSTQSTAVGDATAASGAASSAFGWNSIASGDTSVA
                     IGNHAQSTAAAAQSVAVGNFAFVSGQNATAMGSQAQAAGSNAAAFGAISTASGVSSIA
                     TGYLAAANSFGDIATGAGAVAGAISGGGGNIAIGADSGTVDGAAVPVSAAAIGGDAIA
                     VGTASQGAGFKSTALGAYSSATGNLSTAIGANSQASSFSSAFGESSSASGNSATALGA
                     GSAASGIAATAVGYTSAASGDASSALGANSNASGTAATALGYLSTASATYATATGTAS
                     AASGSSGAAYGYSSNASGGFAVALGALSTASGNSSTATGVFSVASANLTSAYGANSAA
                     SGLNASAFGANARATAANSVAVGAGSTATRANTVSVGAPGAERQITNVAAGTAATDAV
                     NVTQLNAAVGASLGQSVANLENQISENAKAANAGIAEAAALGMIRYDDRPGKFSTGAA
                     VGLHTGQAALALGAGYTTEDGRWRVSAGASLSPTNRNADATVGASATYTWN"
BASE COUNT         2487 a         4697 c         4602 g         2475 t
ORIGIN      
        1 gagggccgga gcgaagcgga ggacccgcca tagcgccgca caagcggata tgatcttgca
       61 cgtagcttgc cggcgctatg gcgggttgac ggcgatcggc cggcgtggcg ccgcggcgct
      121 gaaagattac gtccaagacg caccataact cagactttcc ccaaacagcc ctgcgtgaaa
      181 cggggagaag ggtacgatca cgccgccgcg ttgggcgtca cggtcgcacg gaacttgctc
      241 atcaggtacg gtcccgaaaa aaccggttcg tatttcggcg tctcgcccgg cgcgaggcag
      301 gcttcgaggc aggcgatctc cgcaaaccag ttcggctgat ggaagaaggc cagcgattgg
      361 cgccgcgcca agccgccctc ctccggcggc ggattgacga cgcgatgcag cgtcgagacc
      421 cagcggtcat tggtccagcg ctgcatcaga tcgccgagat tgatgacgaa ggcgccggga
      481 atcggcggca cgggaatcca ggcgccgttc gagaagattt ccagaccctt cgagccctct
      541 tgcggcagaa ggatggtcag gctgccatag tcggtatgcg cgccggcgcg caattggccg
      601 ggcttgggcg gaacgcgctg ctcaggatag ttcagcgcgc gcaacgcgct gattggcgca
      661 tcgatgaagc gggcgaaata atcctccggc aggttcagcg cggccgcgaa gactttcatg
      721 atccgcgccg cgaggtcctc catcgccgca taataagcgc gccaggctgc gacgaagccg
      781 gccggctttt gcggccagat cgtcggcgcg tagcagaaag cgagcgcttc cttgtcggtc
      841 atccccgccg gcgccgaggc cgggccgcca ttgaagctct ccttcaagtc gggcggcgtg
      901 tcgacattgc gcgacttggc caaagcttcg agctcgggcc cgagatagcc gtagggatag
      961 cctttatagg gcgccttggc cttgtccttc tcggcgaaag gcagatcgaa aaacgccttc
     1021 gccttgctcc agacgccgtc gatcacgtcg tccgccacgc catgattggc gatcgcgaga
     1081 aagccggtcg agcggcatat ctcgtcgacc tgccgcccca gcgcgcgacg ccgctcagcg
     1141 ccgcccttct cgaaggctcc gagatcgaaa atagggaatg aggacatggg cgcggccttc
     1201 gtgcggagcc aaactcggcg ccgactatag gcgagcgcgg cctcgtcagc caatcggcgg
     1261 ctctctgccc gaactcttcg atgtctgtta cgttgatgcc catgctcccc gccgccaatc
     1321 tcctcgccga cgcgacctcg ccctatctcc tccagcacgc gcataacccc gtgcattggc
     1381 ggcaatgggg cccggcggcg ctggaggagg cgcgcgagcg cgaccggccg gtcctgatct
     1441 cgatcggcta tgcggcgtgc cactggtgcc atgtgatggc gcacgaatcc ttcgagaacg
     1501 aagagattgc ggcgctgatg aaccggctct tcgtcaacgt caaagtcgat cgcgaggagc
     1561 gcccggacat cgatcacatc tatatgaccg cgctcaacgc gctgggcgaa cagggcggct
     1621 ggccgctgac catgttcctt gccccggacg gcgcgccgct tttcggcggc acctattggc
     1681 cgccggcgcc gcgctggggc cggccgtcgt ttcggcagat tctcctcgct gtcgacaagg
     1741 cttggggcga aaagcgcgag gcgctgctcg cccagggcgc caatctcaag gcgtttttcg
     1801 cagagctttc gcaggccaag ggcggcggcg cggtcggtcc cgccgatctc tcggcgatcg
     1861 ccgcggccta tctgcgccag gtcgatcccg tccatggcgg catcggcggc gcgccgaaat
     1921 tcccgaacgc gccgatcttc cgctttctct ggagcgaggc gttccgcgac aacaaccaag
     1981 cggcgaaagc ggcgacgcgc cgattgctcg tctcgctctg ccagggcgga atctacgatc
     2041 atctcggcgg cggcttcgcg cgctattcgg tcgacgcgga atggcatgtg ccgcatttcg
     2101 aaaagatgct ctacgacaat gcgcagattt tcgaattgct cgccctcgct cacgccgacg
     2161 aacccgacgc gcttttcgcc gagcgcgccg aagagacgtt cggctggctg atgcgcgaaa
     2221 tgcgcgtcgg cgacggcgag cgcttcgcct tcgccgcctc gcaagacgcc gaccaagccg
     2281 gcgaagaagg ctcgttctat acgtggaccg ccgaagagat cgacgccgcc ctcggcgccg
     2341 aaagcgccgc cttcaaggcg gcttacgacg tgcggcccga aggcaattgg gagcatcgca
     2401 atgtgctgcg ccgcacgctc gcggctcatg gcgacgcggc ggcggaagcc cgccttgctc
     2461 aggcgcggcg gaggctgttc gctctgcgcg aggggcggcc gagaccggct ctcgacgaca
     2521 agattctcgc cgattggaat gggctgatga tcgccgctct cgcgcgggct gcgcctttct
     2581 cctttgcgcc ggcctgtctc gacgcggcgc aacgcgcttt cgctttcgtt gacgagaatc
     2641 tgcgcgacaa gaaaggccgc ctggtccatg cctggcgagg ccgcgccggc gccgccggcc
     2701 tgctcgacga ttacgccagc atgtcccgcg ccgccctcgc gctgttcgag gcgacgggcg
     2761 agcgttcgta ccttcgccag gcggtcgcgc tgatcgagga ggcgcgcgat ctctttggcg
     2821 aagcggatgg cggcttcttc atcaccgcac gcgacgccgc cgacgtgccg gccgcccggc
     2881 cgcgccacgc ccatgacggc gcgacgccgt ccggaatcgg cctgatcgcc gaagtcctcg
     2941 cgcggctcca tcatctgacc ggcgagaaaa gctggcgaga cgattgcgag cgtctcatcg
     3001 ccgccttcgc cggtccgcgc gaggcgctcg cccaatcgcc gctgctcctc gccgcagcgg
     3061 attttctcga gcgcgccgca gtcgtggtcg tcggaggaga tccgggcgac ccgcgcgcca
     3121 aggcgctcgc cgcaatcgcc ctcgcgtcgc ccgatcccgc gacttgcctg ctccggacgc
     3181 gcgacggcgc cgactggccg gaaggctcgc cgggctatgg tcgcgcgccg atcggcggcg
     3241 cgccggccgc ctatctctgc cgcggcgctg tttgcagcct gccggtcgcc gcgccggagg
     3301 agcttcgacg actgacggca aaccggagct gagcgctccc tctgcccagc gccgagcgcg
     3361 ccgcgcgctt gcaacctggt ccttcattgt ggcatgctag tcatgactga tccgggcgaa
     3421 agacccggga cggtttcggt cgtccgtaat gccgagcggg cgcgaagaaa aaagggagga
     3481 ttccggaatg aaacatcttg ggcgcgtctc aggcttggcg ttggccggcc tgcttttgtc
     3541 ctcggtcgcg gtcttcgccg gccctaaaac cgtctccggt cccggcgccg atcccgaatg
     3601 cttcaaaccc tggtccaata cgaccaagta tttccaatgg cccaagaagc agggtcccta
     3661 ccggatcgcg gtcgccaacg gctttgtcgg caacacttgg cgcattcaga tgatcaagac
     3721 cgccaaggcg ttcgccgagc tgccggaaat caagaaggac atcaaagagt tcaaggtcgt
     3781 ttcgaccggc accgacgtcg ccgcccagct cggcgcgatg gaggatttca tcaaccaggg
     3841 ttacgacgcg atcatcgcca acgtcgtttc gcccgacggc ttcgaccgca tcatccgcct
     3901 cgccgacaag aacaatgtcg tcgtcgtgcc gttcgacaac gtgctcgaca ccgacaaggt
     3961 gatgcagatc aacgaagacc agaaggaaat gggccgcatg tcggccgagt ggctgctgaa
     4021 ggagacgggc aagacttccg gcgacattct cgaagtgcgc ggtctcccgg gcaactccgt
     4081 cgatcgcgac cgccatgtcg gcttccgcga ggtgatggag aaggcgccca acaagttcaa
     4141 catcaccgaa gtcgtcggca attgggatac cggcacctcg caaaaagtca ccgccgacgc
     4201 gctcgccgtt catggccatt tcgacggcgt gttcacccag ggcggctccg acggcaccgt
     4261 gcaggcgatg atggacgcgc atcatccctt cgtgccgatg tccggcgaag gcgagaacga
     4321 atatcgcaag cagatcgccg agcattggaa acaaggcttg aagggcatgt cctacggcca
     4381 gtcgccggcg ctggtggcga tcgcgatgaa agcggctatt tcggcgctca aaggcaatgt
     4441 catgccgcag ctcatctcga ttccgatccc ggtcgcgacc tacaaggacc tgaagccggg
     4501 cgtgaactat tggcccgact tgaacgtcgg cttcttcgcc gacaatcaat tcaagccttg
     4561 cggcgtcgac ttcaccgcgc cgcagatcat ggcgcagagc gaaaagaaca ctcagtgaca
     4621 ggctgagggc gaaagcgaca atggcccggt tccgcgccgg gccattttgt tgccgccgcg
     4681 acgcgccaaa tattcccgtt caatcatggg cgccatgaaa acagcgacgg ccggcgaata
     4741 tctcgtgctc tcgggagtct ccaagcggta cggcggcgtc cgcgcgcttg agaatgtcga
     4801 tttttcctgc cagcgggggc gaattcacgc ggttctcggc gaaaacggcg ccggcaaatc
     4861 gacgctgatc aagatcatcg ccggcgtcgt ccaacccgat tcgggcgaac tgcgattgga
     4921 tggcgccgtg cgtcagtttt cctatccggc cgccgcgacc gcggccggca ttgtctgcat
     4981 tttccaagaa ttgtcgctga tgccggacct ttcggtcgcc gacaacattt cgatcgcctc
     5041 gccgccgacg cgcttcggcg tgatcgacgc gcgcgcgcaa aggcggcgcg ccgaggctct
     5101 tctcgccgaa gtcggctgtg aggacgtgaa tccgctgctt cgcgtgcgcg acctcccgct
     5161 gtctcgccgg cagatggtcg agatcgccaa ggcgctcggc cagaagccga agctgctcat
     5221 cctcgacgag gcgacctcgg ccctgaccag cgccgatgtc gagaaagtct acgccctgct
     5281 cgcccggctg aaggccgaga atgtcgcgat cctctacatt tcgcaccgga tgcacgaagt
     5341 cgaggcgctt gccgatcgcg cttccgtctt ccgcaacggc cggcacatcg aaacgttcga
     5401 caagggcgcg cgctcgaccg ccgagatcgt ccaattgatg atcggccgcg acatcgccac
     5461 gcaatatccg cccaagccga agcggccccg cccggcgccg gcgctcaaga tcgaaaatct
     5521 cggctgggaa aagcgcctgg aggggatctc gctcgacgtc ggcgcaggag agatcgtggg
     5581 gcttggcggc cttgacggcc aaggccaaag cactttgctt ctagcgcttt tcggcgtcct
     5641 gcgcggcgtc accggctcga tccgagtgaa cggcaaagcg gtgcggcctg gctcgcccgc
     5701 cgccgcaaag gcggcggatg taggcatcgc cctcgtgccg gaggatcgaa agaccgaagg
     5761 cttgatgctg ccgatgtcga tcgccgacaa tctcgccatc gcatcgctcg atgctttgtc
     5821 atcgggccct ttcattgacg ccgccaagga gcgcaaggcg gtcgacgccg gcatcgcgcg
     5881 gctgcagatc aagatcggcg ccaagaccga cgcggtctcc actctttcgg gcggcaatca
     5941 gcagaaagtc gtcatcgcca aatggctgat gactgggccg aaaatcatcc tgctcaacga
     6001 tccgacgcgc ggcatcgacg tcggcaccaa acaggagctc taccggctga tgcgcgagct
     6061 cgccgatcag ggcgcggcca ttctgttcta ttcgacggat tacgacgagc tgatcggctg
     6121 ctgcgatcgg gtcgccatca tgtacgacgg gcgcatcgtg cgcgaactcg cgggggacga
     6181 cttgaccgag acgaacatcg tcgagagcgc gctgaacatc gacccggccg ccgtccggac
     6241 aaagcaggaa tccgctcgtg tctagcgttg catcgccaac ctcgcgaagc gagcgcgcca
     6301 cccaatccga tctcgtcatc cggttgcgtc agaacgccgg cctgatcacg gctgtcgccc
     6361 tattctgcct cgtctacctt ttctatcatc tcgtccatcc gaaagggttt tcctcggcgg
     6421 ttctcgttca gaacgccgac gaggtcttca cgctggcgac tttggccatg gctcagaccg
     6481 tgccggtcct ggcggccggt ctcgaccttt ccgtcggcgc gctgatgacg ctggtcgact
     6541 gcctcgccag ctacctgttg acctattccg acaaagcgca agtgttcaat atcgacatcg
     6601 gcgcgctcca tctgccggtt ctcctcttgc ccggcggcgc cgccggcgtg tggctgggcg
     6661 cgatcatctg cctcctgacg ggccttctcg gcggtttcat caacggttgc gtggtcgtct
     6721 acggccgaat ccagccgatc atcgcgacgc tggcgaccgg cgcgatttat atgggccttg
     6781 cgctctatct gagaccgacg ccgggcggga agatcggcga cgacctgaac tgggcgatga
     6841 ccaacacgct cggcgatttc gcgtccaccg tgcatatttt cgacgacggc gcggcgggct
     6901 ggttcgcgcc gatcgcgggg attccggtac ccgccatcct cctgctgctg atcgcggcgg
     6961 tggtctgggc gccgttccgc cgcaccgtga ccggacggac ggtctatgcg atcggctccg
     7021 ccgaaggcgc ggcctatatg tccggcctgg cgatccagcg cgcgaggctc gccgccttca
     7081 cgctcggcgg tttcttcgcc ggctgcggcg gtttgttcct ggcgatccag acctcgtcgg
     7141 gcaacgccga cattccccag gccggagaat atacgctgaa ctcgatcgcg gcggtcgtca
     7201 ttggcggcac gtcgctgctc ggcggctccg gcggcgctat cggctcgctt ttcggcgcgc
     7261 tcatcctccg agtcatctcg ttctgcttcc ggattttcga cgtcccgcct ctcctgcagc
     7321 cgctttttga gggaatcgtc ctcctcgtcg ccgtgagcct cggcgccttg cgcgtcctgc
     7381 gcgtcaagaa cacgctggag ctgttccgat gacgaccggg agcttgacgc tgtcgcgcga
     7441 cgatcggccg atcgcgatcg ccgctctgtt catcgtggcg attctgattg caggaaccgc
     7501 ctatacgctg gagcgattcg gcagcgcccc gctgctctcg cccaactacc tgcttcagca
     7561 attgcagatc ggcgcctttc tcggcatcgt ctccgccggg atgatgatcg tcatcctcct
     7621 cgcgcatatc gacctctcgg ttccttggac tttgaccgca gcggcgatga tggcgaccgc
     7681 cgtaagcgga ccttgggcgc tgccggtcgg actcggcgtc ggcctcgccg tcggaaccgt
     7741 caacgggctc ggcatcgcct atctgcgcgt gccctcgatg atcttcacgc tcggcgtcaa
     7801 tgcggtgatg cgtgggctga tggtcgctta taccggcgga ttcgccccgg acacccacgc
     7861 gacggacctg atgcgcacgc tcgccgccga gcggactttc ggcatgccga acgcgctgtt
     7921 cgtctggctc gccgtgtcgg cgatcgtggt gatcatcctg cagcgcaccg cgctcggccg
     7981 ctacgtctat gcgatcggca atcgggaggc ggccgcctat ctcgccggcg tcaacacccg
     8041 ccacgtcatc gtgatctgct tcgcgctttg cggcctcgcc tccggcctgg ccggcgtcct
     8101 gctcgccggc tattcgcaga aagcgtacca aggcatgggc gatccctacc tcttgccctc
     8161 gatcgccgcc gtcgtgatcg gcggcaccaa tatccttggc ggccgcggcc gctatctcgg
     8221 cacgctggtc ggcgtgatcc tgatcgtgct gttgaacagc gttctctcga tcatggagat
     8281 gcccgaggcg ggtcggcagg tgatctatgg cggggtgatc atcctgatgc tgctcgtcta
     8341 tggccgcggc gagcgcgtga cgagctgaaa gcagcgacaa tggcgggtgg cgtgcgggcc
     8401 cgcctccggg gcggtcatgt tttggaatat cgctccgcga gcgctggata atttcaataa
     8461 gctcttgcca aaaatcgtgg cgcaaaccat tctgaggtca tggcgaaagc gtcgaagaag
     8521 acgaaacgcg gcaacaaggc ggagagggcg gccggtcagg ccaaggtgat tggcgtgacc
     8581 aaggacggcg ttcgcatttt gaagccgagg ggaaaggcga ctcacttcac ccagaaggaa
     8641 attcgcgaag ccgtagccgc cgccaggacc gcccggcgag cctaatgggc gcgcgcgacg
     8701 tttttattaa ctgtccattt tcggcagact accaggaatt ttttgaggca atcgtctacg
     8761 ccgtcatacg gtcgggcttt acgccccgtt gcgcgcgcga gaatgacgat ggcggggaag
     8821 tgcggatcga caaaatctgc cgaatcatcg gcgaaagtcg atacggaatc cacgatattt
     8881 caaagaccga actcgaccca gcctctgggc tgccccgttt taacatgccg ttggagctcg
     8941 gccttttcct tggggctcgc aagttcggcg gcagaggcaa cgccaacaag aaagcgctca
     9001 tcctcgatcg cgaaagccac cgctatcagg catttatttc cgatattgcg gggcaagaca
     9061 tccactccca caacggtaat gtgcgtcgag cgatcgaatg cgttgcaact tggctacggg
     9121 acgaagctgg agatcctgac gtaccgggcg gccgcgctat tgcgacggaa ttcgaacgat
     9181 tccgcggagg ccttccaagt atcgccgagt ccaagcgcct ggagcccgac gagctgactt
     9241 tcagagattt gacggcaatc gctgcggaat ggattctggc cgaaagcgga tcaaacggct
     9301 cctcctcacc tttgtaactg cgccgtcgtc ttgccgacaa gccaagctcc ccccagtaac
     9361 ggactaacca agggagcaac agcccctacc ccgccgattt catcgcctcg gcggcgagga
     9421 agtcgcgcaa ggcggagatg cgcggcgaat tcgcctgctc ggggcggcag aggaaataat
     9481 agccgtaggg cgtatgcact tccgtcgtga acggcgcgac gactcttccc gccgcgagct
     9541 cgtcggcggc gatggcgagc gtggtcagcg tgacgccctg gccttgcgcc gcggcgtaga
     9601 gcgccatcga catatggttg aaccaaacgc cgcgcgaagc gtcgacctcc gcggcgcccg
     9661 ccgctttcag ccagtccgcc caggtcggcc aggtcgaaaa gctcggattc cagtccaggt
     9721 gtaaaagcgt gtgcgacttc aaatcctccg gcgcgcgcaa tggatttgcg ccctcgatca
     9781 ggcgcggcgc gcagaccgga aagacgttct cctcgaacag cagcgtcgtc gcgaagccgg
     9841 gaaactcgcc ggcgccccag cggatcagcg catcgactcc gggatgatcg agcggctctt
     9901 cgagcggatt ggcgtcaagc aggacgtcga tgtccggatg agccgctttg aaccggccga
     9961 tgcggccgac cagccaggtc gcggcgaagg acgggctcga attgatcacc agaaagcgcc
    10021 ggcccgaagc ctcgcgcatc gcgcgcgtgg cgcgggcgag gaattcgaag ccctcggcga
    10081 ggtcgcgcat ccccgcctgc cccgcctcgg tcagccttaa gccgcgtccg gcccgttcga
    10141 ccaggatcgc gccgatctcc gtctcgagct gccgcacctg ctgggcgacc gccgccttgg
    10201 tgacgaaaag ctcgtcagcg gcgcgggcgt aggaaaggcg gcgcgaaacc gcctcgaaag
    10261 cccgcaacgc gttaagggag ggcagccggc tcggattctt catcgtatag tttttcttgt
    10321 tggttcacac ataaagcatg acttgagaat tgtcgagatc ggcgtatatc agcgacaaat
    10381 cctggggcat tcggtattcg gggctttaaa atgatccatt cgcgtcaccc aaacgaaaat
    10441 ccaatcgccg gcttcggcgc tctcggccgc agcgtatgta aacaaattct taaccgcctg
    10501 gcggaagcgt cgaaccgcgc ggtggtccgc agccgtttcg ccgacctgca gctccgcgat
    10561 ctcgaagata tcggcctgac gatcgccgag cgggacgcca tgctgcgctg agatttcccc
    10621 ggcctcgccg ccaaagccgc gaggccgggc ttgcgcccgg cttttgctta tgccggatcg
    10681 aaagtcgcaa aaacgctgac cagcgccagc acgacgccgc caagcccgat ctcgatcgcc
    10741 gtgttgcggg caagggccag cctcgcctcg ggcttcagcg ccaggcgcgg cgctagctga
    10801 tagcggttat agagcgccaa caggatcatc gccgcgacgg tcaaaatttt catcgccaag
    10861 ctggcctggt agggcgaagc gaaattggtc ggcggccggc cgagaatcat gaacgtattg
    10921 gcgattccgg tcgccagaac cgccgcgacg gcgacgtggc cgagcccgga gaaccggcgc
    10981 agcgccacgc ccgcctgagc ctcgaatccc ggctcgccga gccggcgcaa gcaaatcaag
    11041 agcggcggca gcgcgccgag ccagaaaccg ccggctagga ggtggagggc ctgattggcg
    11101 cgctcgaaga ggcccgccgc atgctccagc atcgcggcat gaccgaccag gccgagactc
    11161 gcgaggaaaa gacccgatag cgcggcgatg atggccggcg gaacgccgcg ccaaaacgcc
    11221 agaacaagca gcagcagggc aagcgcgagg cgcgcccgcc agacatggcc gaagccggtc
    11281 tcctcgagca ccgccgccag cgcgtccgtt tggagcgcgg cgcgccagtc gccggtcatc
    11341 aggccggcct ccaacagaag ccaaagcgcg gcgctcacgg cggcggcgac gctggcgacc
    11401 acgatgatgc gcttgagcgg aggcgcgact tgcgcgcgca aaagcggcgg cgcaagcgcg
    11461 gcgagaaaca cgctcgcgcc gaacaaggcc atagccgagc cgaaatggac gaaacggacg
    11521 acgatcagga aaaggttcaa aagatcgcgc cctatccgat cgtcccttcg cggaaaaccg
    11581 cttcggcccg ctgctcggta tggcgttcaa ccatgggaga gtcgctcgcg gttgttgcga
    11641 tcagcgacga cacaatagac gcgccatttg acgatttgaa gccgggaaaa caaagtcggt
    11701 tgcaatgcga cgcgccgtcg caacctcccg tcatgcctgg ccttgcgcca agcattcgcg
    11761 cccgcccgca gccgcaaacc ctggaaagtg tttgcagtgg cgccgcgtgg atggccggga
    11821 cgaacccggc catgacgcat tctgactgcg ccgggcgcca gcgctcgcgc cgtccgcgcg
    11881 taccttcttt cccgactgcg ctttcgtgaa cacattgcgc ggcaggttgc gattggcgaa
    11941 aggcgattat ttggggcgca aaacggttac ggcgatgaca atacagccat tgcccgccga
    12001 tggcgcgagc agatagcgaa cccctgccgg attctcgatc agcggcagac cttcgagatt
    12061 ggcgacggcg cggccggaat tcatcaacgc ggcttgaata gcgccgcaag agcgcgccgt
    12121 cggaacgacc tgtacgcccg ccgcttcgca ttcgcccgat ttcgtcggcg tcgcgagcac
    12181 gatagaggcg ccgcgcggcg ccgacggctg cggatagctg agcgccgcga tcgacccgaa
    12241 aaggtgatcg tcggcgtttt ggctcgacca gaaggagaag gcgtcgtgcg gcgcgtcgac
    12301 gacctgaggc agcaggcgcg tcaatgcgtc cgtgcaagcg gcgactccgg cttggcgcgc
    12361 atggacgagc gcctgaaaca gcggcgcttg cgggacctcg cccggttgaa ggaccggcgg
    12421 cggcgggtct tttttctcgg cggccttacc cttgtcttgt cccgggctgg ccggtttctt
    12481 ctctgacgcc ggctcggcgc gtttttccgg agctttggcg ataggctttg gcggggttcg
    12541 tttcggccgc gccggatcaa cgcctgaagc gggcgacgcc tgatcgagcg agggcgcctg
    12601 cccggtcgcc ggccttgcat agaccagaag gcttgcgacg ccgaatagcg acagcgcata
    12661 agccggccat ggcgacgcaa tgttacaccg ccggcggcgc cgccgctcat ggttttccgt
    12721 cctttcggct gccgctcgcc gcatcgggcc gccgcctaat tccaggtgta ggtcgcgctc
    12781 gccccaacgg tggcgtcggc gttgcggttt gtcggcgaga gcgacgcgcc ggccgagacg
    12841 cgccagcggc cgtcttcggt cgtatagccg gcgcccaatg cgagcgccgc ttggccggta
    12901 tgcaacccga ccgccgcgcc agtcgagaac tttcctggcc gatcgtcata gcggatcatg
    12961 cccaaggcgg cggcttcagc gattcccgca ttggccgcct tcgcgttctc gctgatctgg
    13021 ttttcgaggt tggcgacgct ttgacccagc gaggcgccga ccgccgcgtt gagttgggtt
    13081 acgttcaccg cgtcggtcgc cgccgtgccg gcggcgacat tggtgatctg cctctcggcg
    13141 ccgggcgcgc cgaccgaaac cgtattggct cgcgtcgccg tcgagcctgc gccgacggcg
    13201 accgaattgg ccgctgtcgc cctggcgttg gcgccgaagg cggaggcgtt gaggccggag
    13261 gcggcgctat tggcgccgta agccgaggtc agattggctg acgcgacgct aaagacgccc
    13321 gtcgcggtgg aagagttgcc ggacgcggtt gagagcgcgc ccagagcgac ggcgaagcct
    13381 cccgaagcat tgctgctata gccataagcc gcgcccgagg agcccgacgc ggcgctggcc
    13441 gtgccggttg cggtcgcata ggtggccgac gccgtgctga gatagccgag cgccgtggcg
    13501 gcggtccctg aagcattgct gttcgcgccg agcgccgagg acgcatcgcc cgaagccgcg
    13561 ctggtatagc ccaccgccgt ggcggctatg cccgaggcgg cgcttccggc gccgagcgct
    13621 gtcgccgaat tgcccgaggc gctgctgctt tcgccaaatg ccgagctgaa gcttgaggcc
    13681 tggctattcg cgcctatggc ggtcgaaaga ttgcccgtcg ccgagctgta ggcgcccagg
    13741 gctgtcgatt taaagcccgc gccctgggaa gccgtgccga cggcgatcgc gtcgccgccg
    13801 atggccgcag cgctcaccgg aaccgcggcg ccgtccacgg ttcccgaatc ggcgccgatg
    13861 gcgatgttgc cgcctccgcc ggaaatcgcg ccggcaaccg ctccagcgcc ggttgcgatg
    13921 tcgccgaaag agttggctgc ggcgaggtaa ccggtcgcta ttgagctgac gccgctggcg
    13981 gtgctgatcg cgccgaaagc cgccgcattc gagcccgccg cctgagcttg actgcccatg
    14041 gccgttgcgt tctgccccga aacgaacgca aaattgccga ccgcgacgct ctgcgccgcg
    14101 gcggcggtgg attgcgcgtg attgccgatc gcgacggaag tgtcgccaga cgcgatcgaa
    14161 ttccatccaa aggccgagct ggcggcgccc gacgcagcgg tcgcgtcgcc gaccgcggtg
    14221 ctctgggtac taggggcggt ggagccgttt ccgacggcca a
//
LOCUS       DATGJW010000531         2700 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1348436, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000531 DATGJW010000000
VERSION     DATGJW010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2700)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2700)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2700
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1348436"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(158..1744)
                     /locus_tag="VKU03_16165"
     CDS             complement(158..1744)
                     /locus_tag="VKU03_16165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003611317.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucan biosynthesis protein"
                     /protein_id="HLJ72851.1"
                     /translation="MPPLNRRALLVAMISASAARWTMPAVAQQNPPSPPAAAGPAAAP
                     HFGYDDVVKKARDLAAAPFDGRVAPLPDGLAHLDFDAWRDIRFRPDKALLGQNGDPFR
                     LELFHLGHLYKRPVVINILRDGIPAPIPYAANLFDYGHNKVDANLPINLGFAGFRLHY
                     PLNSPKVMDEVIAFLGASYYRFLGRAQRYGLSARSLGINTAVGQTEEFPFFREFWIDT
                     PEPGAQRAVIYGLLDSESASGAFRFELYPAQETTIEVTATVFARREIQTLDMAPLSSM
                     FLLGKNDHRIADDFRNELHDSDGLLIHNGSGEWIWRPLSNPVEPEMSVFLDKDIRGFG
                     LLQRDRNFADYQDIDLAYELRPSYWIEPRENWGEGRIELLELPTTDESNDNIVASWVP
                     KEPMEAGRTLSYGYRITALLNDARLAPGGRAVATYRTHPRALGSIEPLAPGSTRFIID
                     FSGGDLAYYQAQPSLVEVVPSASKGKITRSFLTANPHIQGFRAGVDVQIDPGQASDLR
                     AFLKAGNRALTETWTFPWRA"
     gene            complement(1859..2437)
                     /locus_tag="VKU03_16170"
     CDS             complement(1859..2437)
                     /locus_tag="VKU03_16170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72852.1"
                     /translation="MLTAVAVCGVLWAGGRAEAQTSSTPAEPTLPSLALNPEAPSPWA
                     GFYFGSEVEAAFARGSKPGFGGAAFAGYNRELPNNLVIGVQGSAGYAPYLFASGPGHG
                     FNFAETSAKIGYDMGRFMPYVTAGVVFAKANSFGVPAYTGAADSMNALFAPGGDLRAF
                     GVVGAGFDYAINNNLSFGMEVRAGAGRAPLAP"
BASE COUNT          496 a          815 c          970 g          419 t
ORIGIN      
        1 ggtaggccgg tttaaaccgc gaaaccctcc cgcacccccc ccgctttgcg ggcgagcgtc
       61 gcgtttgtac ctccaagccg cgccgcgcgc ggtcgttttc cgccgcggct gggcctccct
      121 ctcccgcgaa gcgggggagg tacgggggcg gcctcggcta cgcccgccag ggaaaggtcc
      181 aggtctcggt cagcgcgcga ttgccggcct tgaggaaggc gcgcaagtcg gacgcctggc
      241 cgggatcgat ctgcacgtcg acgccggcgc ggaagccttg gatgtgcgga ttggcggtca
      301 ggaacgagcg tgtgatcttc cctttagagg ccgacggcac gacttcgacc agcgatggct
      361 gggcttgata gtaggcgaga tcgccgccgg aaaaatcgat gatgaagcgc gtcgagccgg
      421 gcgcgagcgg ctcgatcgag ccgagggcgc gcggatgggt gcgataggtg gcgacggcgc
      481 ggccgcccgg cgcgagccgc gcgtcgttca aaagcgccgt aatgcgatag ccgtagctca
      541 gcgtgcggcc ggcctccatc ggctccttgg gaacccagga ggcgacgatg ttgtcgttgc
      601 tctcgtcggt ggtcggcagt tcgagcagct caatgcggcc ttcgccccaa ttctcgcgcg
      661 gctcgatcca atagctcggc cgcagctcgt aggcgaggtc gatgtcctga tagtcggcga
      721 aattgcggtc gcgctgcagc aggccgaagc cgcgaatatc cttgtcgaga aacaccgaca
      781 tttccggctc gaccggattg ctcagcggcc gccagatcca ttcgcccgat ccgttatgga
      841 tgagcaaccc gtcggaatcg tgcaattcgt tgcggaagtc gtcggcgatg cgatgatcgt
      901 tcttgccgag caggaacatc gacgacaagg gcgccatgtc gagcgtctgg atttcgcgcc
      961 gcgcgaaaac cgtcgccgtc acttcgatcg tcgtctcctg ggcggggtat agctcgaagc
     1021 ggaaggcgcc ggacgccgat tcgctgtcga gcagcccgta gatgacggcg cgctgcgcgc
     1081 cgggctcggg cgtgtcgatc cagaattcgc ggaagaacgg aaattcctcg gtctggccga
     1141 ccgcggtgtt gatgccgagg ctgcgcgccg agaggccata gcgctgggcg cggccaagaa
     1201 agcgatagta gctcgcgccg agaaaggcga tcacctcgtc catcactttc ggcgaattca
     1261 gcggatagtg cagacggaag ccggcgaagc cgaggttgat cggcagattg gcgtccactt
     1321 tgttatggcc gtaatcgaag agattggcgg cgtagggaat gggcgccgga atgccgtcgc
     1381 gcaggatgtt gatgacgacc ggccgcttgt agagatggcc gaggtggaac agctccaggc
     1441 ggaaggggtc gccgttctgg ccgagcaatg ccttatccgg acggaagcgg atgtcgcgcc
     1501 aggcgtcgaa atcgagatga gcgagcccgt ccggcagcgg cgcgacgcgg ccgtcgaacg
     1561 gcgcggcggc aagatcgcgg gctttcttga cgacgtcgtc atagccgaaa tgcggcgcgg
     1621 cggcgggacc ggccgcggcg gggggcgaag ggggattttg ctgagcgaca gccggcatgg
     1681 tccaacgcgc tgccgaagcg ctgatcatgg cgacgagcag cgcgcggcga ttgagaggcg
     1741 gcatggggtc caacaaaggc gagcggataa gcgacctata gcgggcggcg cgagggctcg
     1801 gcaacagcta agctcggcag agccgccatg cgccgaacaa accggccgag tacaaagatc
     1861 acggcgcgag tggggcgcgt ccggcgcccg cccggacctc catgccgaag ctgagattgt
     1921 tgttgatcgc atagtcgaag cccgcgccga cgacgccgaa cgcgcgcaga tcgccgcccg
     1981 gcgcaaacag cgcattcatc gagtcggcgg cgccggtata agccggcacg ccgaaggaat
     2041 tggccttggc gaagacgacg ccggccgtca catagggcat gaagcggccc atatcgtagc
     2101 cgatcttggc gctggtctcg gcgaaattga agccatgccc ggggccgctg gcgaaaagat
     2161 agggcgcata gcccgccgaa ccctggacgc cgatcacgag attgttgggc agttcgcggt
     2221 tatagccggc gaaggcggcg ccgccgaagc cgggcttcga gcctctggcg aaagccgcct
     2281 cgacctcgct gccgaaataa aagccggccc agggcgacgg cgcctcgggg ttgagcgcga
     2341 gcgagggcaa agtcggctcg gcaggcgttg aactggtctg agcttcggcg cggccgcccg
     2401 cccataagac gccgcatacg gcgacggccg ttaacaatct cagcgccatg cgcccctcac
     2461 ggatttgtcg ttggcgatca tattcagcag gcaagtgcgg cgtcaaagcg gcggtccggg
     2521 cgaaaggcgt ttatgcgcag acgcggcgct tgccaagcga aagcccgccg gaaaagaatt
     2581 gcgcgcggtg cggcggcgcg cctcggagca aagcgagttc ggagcatggc gggacgaggc
     2641 gcgttcgacg gacccgttct gcttggcgtc gatgtgggaa cgcagtcgat ccgcgccctc
//
LOCUS       DATGJW010000532         3945 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_723619, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000532 DATGJW010000000
VERSION     DATGJW010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3945)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3945)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3945
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_723619"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..769
                     /locus_tag="VKU03_16175"
     CDS             <1..769
                     /locus_tag="VKU03_16175"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF023780.4"
                     /note="catalyzes the DNA-template-directed extension of
                     the 3'-end of a DNA strand; the tau chain serves as a
                     scaffold to help in the dimerizaton of the alpha,epsilon
                     and theta core complex; the gamma chain seems to interact
                     with the delta and delta' subunits to transfer the beta
                     subunit on the DNA; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HLJ72853.1"
                     /translation="RLAAIGAATSAGTLARMWQMLLKAHEEARRAPDPADAAEMALIR
                     LAYAADLPSPEEALRKLAESGDRPAVAPLAAPRGPAGGASAALRAEPRPAPAAAPQAA
                     AAPGPRLARFEDVVALARSKRDIQLQAALERDVRLARFEPGSIDFSLIEGASPQIAQT
                     LARRLQEWTGERWMVALVAGAEAPTLREVAEAKEKERLSGVAAHPLVQKVLQRFPGAE
                     IVDVRRPDAPGDTPQSGAPAVDDDVAYGDVSLGEDDF"
     gene            783..1106
                     /locus_tag="VKU03_16180"
     CDS             783..1106
                     /locus_tag="VKU03_16180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003614007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HLJ72854.1"
                     /translation="MPDLMGLMKQAQAMQAKLQEAQAELDHIEVEGQSGGGMVRVILS
                     AKGAMKGIVIDPSLMKAEEKEIAEDLIVAAHADARAKAERVLEEKMKAMTAGMALPPG
                     LKLPF"
     gene            1339..1944
                     /gene="recR"
                     /locus_tag="VKU03_16185"
     CDS             1339..1944
                     /gene="recR"
                     /locus_tag="VKU03_16185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014890200.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HLJ72855.1"
                     /translation="MAESVAGPEIERLIQLLARLPGLGPRSARRAALHLIRKKEALLA
                     PLAEAMRTAHERVTTCAVCGNVDTRDPCTICADPRRDAATLVVVETVADLWALERAGV
                     VRARYHVLGGVLSPLDGVGPEDLNFSSLVARVAEGAVKEVILAVNATVDGQTTAHYVI
                     DLLAAHNVKVTRLAHGVPVGGELDYLDEGTLAAAINQRTAF"
     gene            1995..2237
                     /locus_tag="VKU03_16190"
     CDS             1995..2237
                     /locus_tag="VKU03_16190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013893674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0051983 - regulation of chromosome
                     segregation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system ParD family
                     antitoxin"
                     /protein_id="HLJ72856.1"
                     /translation="MPRSASISLGDHFDDFIESQVAAGRYDNASEVVRAGLRLLEERE
                     ARLAALRGALIEGERSGASEPFNFDDFIERRLGEAS"
     gene            complement(2425..3378)
                     /locus_tag="VKU03_16195"
     CDS             complement(2425..3378)
                     /locus_tag="VKU03_16195"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HLJ72857.1"
                     /translation="MASSLVAKPPPHGQNTVAAAVQRRRAIAIAIMCAAMVCFTGHDA
                     SAKWLARSLPTVEIVWARYVAAAIFGLIIMRPIKRPALLRSRRPYLQALRSLMLLGSS
                     MANLVAIRHLQLAETATISFLQPLLVALIAGPLIGEWVGPARMAAIGFGLVGVFVAMA
                     PGTHAFQPVALVAIGGVFCNTFYAIFTRMLAAHDAAETTLFFTPLAGVAALTPLLPFV
                     WVPPPTLFALGVVLLMAVCATAGHGLLILAHQRAPAPILAPFAYTQLLWMVGAGLIVF
                     GDRPPPTVLTGAAIVVACGLFLVWREKSGGRTPVVDVVDAR"
     gene            3803..>3945
                     /locus_tag="VKU03_16200"
     CDS             3803..>3945
                     /locus_tag="VKU03_16200"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72858.1"
                     /translation="MRDLKGRTAFVTGGASGFGLEFAKLFLAEGMKVFLADIEAPALA
                     RAL"
BASE COUNT          679 a         1301 c         1365 g          600 t
ORIGIN      
        1 gcgcctggcg gcgatcggcg cggcgacctc ggccgggacc ctggcgcgga tgtggcagat
       61 gctgctcaag gcccatgagg aggcgcgccg cgcccccgat ccggcggacg ccgccgaaat
      121 ggccctgatc cgcctggcct acgccgccga tctgccgtcg ccggaagagg ctttgcgcaa
      181 gctcgccgag agcggcgaca ggccggcggt tgcgccgctc gccgcgccgc gcggccccgc
      241 cggcggcgcc agcgccgcct tgcgcgccga gccgcggccg gcgccggccg cggcgcctca
      301 ggcggcggcc gcgccggggc caagactcgc ccggttcgag gacgtcgtcg cgctggcgcg
      361 ctcgaagcgc gacattcaat tgcaggccgc cttggagcgc gacgtgcgcc tcgcgcgttt
      421 cgagccgggc agcatcgact tctcgctgat cgagggcgcc tcgccgcaaa tcgcccaaac
      481 gctcgctcgc cgcctgcagg aatggacggg cgagcgctgg atggtcgctc tcgtcgccgg
      541 cgcggaagcg ccgactttgc gcgaagtcgc cgaggccaag gagaaggagc gcttgagcgg
      601 cgtcgccgcg catccgctgg tgcaaaaggt gctgcaacgc ttccccggcg ccgagatcgt
      661 cgatgtgcgc cggccggacg cgccaggcga tacgccgcaa tccggcgcgc cggccgtcga
      721 cgacgacgtc gcctatggcg acgtttcgct cggcgaggac gatttttagg agctttacgg
      781 acatgcccga cctcatggga ttgatgaaac aagcccaggc catgcaggcc aagctgcagg
      841 aagcgcaggc tgagctcgac cacatcgagg tcgagggcca gtccggcggc ggcatggtgc
      901 gcgtgatctt gagcgccaaa ggcgcgatga aggggatcgt catcgatccg tcgctgatga
      961 aggcggagga aaaggagatc gccgaggacc tgatcgtcgc cgcccatgcc gacgcgcgcg
     1021 ccaaggcgga gcgcgtgctc gaagagaaga tgaaggcgat gaccgccggc atggccctgc
     1081 cgcccggcct gaagctgccg ttttgagagc ggcgatgtgg tttagcgctt ttggcgcggc
     1141 gcaaatccct tctccccgct tagcggggag aaggtggccg cgcgaagcgc ggccggatga
     1201 ggggctgcgc aggactcgaa agacgagcgc tctccgaacg ctaatcccca attcagcgca
     1261 gcccctcatc cggccgcttc gcggccacct tctccccgct tcgcggggag aagggatacg
     1321 ccaagcttcc gcttgaccat ggccgagagc gtcgccgggc cggagatcga gcggctcatc
     1381 caattgctgg cgcgcctgcc gggcctcggc ccgcgctcgg cgcgccgcgc cgcgctgcat
     1441 ctcattcgca agaaggaggc tttgcttgcg ccgctcgccg aggcgatgag aaccgcgcat
     1501 gagcgggtga cgacctgcgc cgtctgcggc aatgtcgaca cgcgcgaccc ctgcacgatc
     1561 tgcgccgatc cgcgccgcga cgccgcgacg ctggtcgtgg tcgagaccgt cgccgacctc
     1621 tgggcgttgg agcgcgccgg cgtggtgcgc gcccggtacc atgtgctcgg cggcgtcctc
     1681 tcgccgctcg acggcgtcgg gccggaggat ttgaactttt cgtccctcgt cgcccgcgtc
     1741 gccgagggcg ccgtcaagga ggtgatcctc gccgtcaacg ccacggtcga cggccagacc
     1801 acggcgcatt acgtcatcga cctgctcgcc gcgcacaatg tcaaagtgac ccgcctcgcc
     1861 catggcgtgc cggtcggcgg cgaattggat tatctcgacg agggcacgct ggccgcggcg
     1921 atcaaccaga ggacggcgtt ttagccggtt tgagcgaatt cgcccatctg gtatattgct
     1981 tcacggagat tgtcatgccg cgcagcgcct ctatcagcct cggcgatcac tttgacgact
     2041 tcatcgagtc gcaagtcgcc gcgggtcgct acgacaacgc cagcgaggtc gttagggcgg
     2101 ggctgcgatt gctcgaagaa cgcgaggcgc ggctcgcagc attgcgcggc gccctcattg
     2161 agggcgagcg gagcggcgca tccgagccgt tcaatttcga cgattttatt gagcgccgcc
     2221 ttggcgaagc atcttaatgc ggtcgccgcc aaccccctcc ttggacgctc ctgcgagcat
     2281 gtccggccgg gctacaggaa attttccggc tggatcgcat gtactgtttt atcgagccga
     2341 agaatcctgc attaatgtcg tacgtatctt gcgcgcgcga atggatgttg atcggcagtt
     2401 ggagttcccg ctcgtcgccg accgtcaccg cgcgtccacg acatccacga ccggcgtcct
     2461 gccgccgctc ttctcgcgcc agaccaggaa caggccgcag gcgacgacga tcgccgcgcc
     2521 ggtcagcacg gtcggcggag gccggtcgcc gaagacgatc aggccggcgc cgaccatcca
     2581 gaggagctga gtataggcga agggcgcgag gatcggcgcc ggcgcgcgct ggtgggcgag
     2641 gatcagcaag ccgtgcccgg ccgtcgcgca gaccgccatc aggagaacga cgccgagcgc
     2701 aaagagagtc ggcggcggga cccaaacgaa gggcaaaagc ggcgtcaacg ccgcgacgcc
     2761 ggcgagcggc gtgaagaaca aggtcgtctc cgcggcgtca tgcgcagcca gcatgcgggt
     2821 gaagatcgcg tagaacgtgt tgcagaagac gccgccgatc gcgaccagcg cgaccggctg
     2881 aaaggcgtgg gttcccggcg ccatggcgac gaacacgccg accaggccga aaccgatcgc
     2941 cgccatccgc gccggcccga cccattcgcc gatcagcggg ccggcgatca gcgcgacgag
     3001 cagcggctgc aggaaggaga tcgtcgctgt ctcggccaat tgcagatggc ggatcgcgac
     3061 caggttggcc atgctcgatc caagcagcat cagcgagcgc aaggcctgca gatagggccg
     3121 gcgcgagcgc aacaaagctg gacgcttgat cggccgcatg atgatcaggc cgaaaatcgc
     3181 cgccgcgaca tagcgcgccc agacgatctc gacggtcggc aaggatcggg cgagccattt
     3241 ggcgctcgca tcatgaccgg tgaagcagac catcgcggcg cacatgatcg cgatggcgat
     3301 ggcgcggcgg cgctgcacgg cggcggcaac ggtgttttgc ccgtgaggcg gcggcttcgc
     3361 gacgagcgaa gaagccatat caaagtcttt gaacaaagcg gctttaggcg cctcgcgcag
     3421 cggccgaggc gcgttccttt tgagattcgc gtggcgggag gtttagaccc ggcgggcggt
     3481 ttcggcaatt gaggcgcggc cggcgctcct cttcaaagcg ccaaaaagcg caatccgtca
     3541 ttgttggaac gctggcccct gtgcgccgag cgaaaaggct gtcaaggacg cgcgctttgt
     3601 gcgcgccgcc gaaggcggtc gcgaagcgat ccttgacagc ctttccgctc gacgcgttgg
     3661 cttggcgttc caacaatgac ggattgcgct cttccgccgg cgaaactcca ttgaaagcga
     3721 gcttgcttat cggcgcagag aaacccgcaa tgacgcggcg agcgcttccc gttacagtcg
     3781 cgccgcccag gggagaaact ccatgcgcga tttgaaaggc agaacggcgt tcgtcaccgg
     3841 cggcgcgagc ggctttgggt tggaattcgc caagctgttc ctcgccgaag gcatgaaggt
     3901 cttcctcgcc gacatcgaag cgccggcctt ggcccgcgcg ctcga
//
LOCUS       DATGJW010000533         2028 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1034111, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000533 DATGJW010000000
VERSION     DATGJW010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2028)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2028)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2028
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1034111"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            443..886
                     /locus_tag="VKU03_16205"
     CDS             443..886
                     /locus_tag="VKU03_16205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72859.1"
                     /translation="MLARSLFSIQAAFLSLFVCAAPACAETFVFTGVAQSADIIDPNV
                     LEYGQTYPIPSPAYSVLRGYPHASWYESWRLRRSPSPVALYPHPCAWANELVELATPC
                     NFVAVPVTTVVEAPLMPLAHRHYRHARAYRSCLCRRRHAVDPPTP"
     gene            1002..1787
                     /locus_tag="VKU03_16210"
     CDS             1002..1787
                     /locus_tag="VKU03_16210"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320012.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1194 domain-containing protein"
                     /protein_id="HLJ72860.1"
                     /translation="MRVTRRRAAALLSSASVLSLIGGKARAGDPVDAAIVLAADVSRS
                     INDEEFGLQRQGYAVAITNARVMEAIRSGPRAAIALCFLEWAGEGEQRVLVEWATIKT
                     DADASRFAAALSAAPRAFVGRTAIGSAIDFSMGVLGESGIAADRRVIDVSGDGTSNQG
                     RFVTEARDAAVKTGVVINGLAIFNRRAAAMGGYLALHTNPSGGLAKYYHDNVIGGPGA
                     FVLPIDDFKSFGEAMIRKLVSEIAGAYPPRSAQRGEGGGEAAG"
BASE COUNT          303 a          707 c          639 g          379 t
ORIGIN      
        1 gcaggctgcg actcctctgg accgaatcct taaggcttcg cctcctccca tcccgtcggc
       61 gtccagcgca gcaccgccgt cgagagcggc ttctgcgccc gcgccgcgcg tcgatcgttt
      121 ctccatcgcc agccttctcc cgccccttgc atttcgttgg atcgtaccgg acgttttgga
      181 gtatagcgtc aggcgcgacg aaatgacgtg gcgtcgcctt tgttcttgcc gcgacgcgcc
      241 ggctttcatc gcaaaagccg gccgaacggt acgacggagc gcatggctca atgcgattgt
      301 cgcgccgtcc agcgcggcgg cgcctcatta aatccgctcg gacatacgca aatttgaaca
      361 gtctccattt acctcccgta aagccgattg gcgcaggctt gaggcaggcg aggggcgttc
      421 cgcgggacgg aggaaccggt ccatgcttgc tcgctctctc ttttcaatcc aagcggcgtt
      481 tctgagcctt ttcgtctgtg cagcgcccgc atgcgccgag acgttcgtct ttaccggcgt
      541 ggcgcaaagc gccgacatca tcgaccccaa tgtgcttgaa tacggacaga cctatccgat
      601 cccgtcgccg gcctattcgg tcctgagggg ctatccgcac gcgtcctggt acgaaagctg
      661 gcggctgcgg cgaagcccca gcccggtcgc gctctatccg catccttgcg cttgggcgaa
      721 tgagctggtc gagctcgcca cgccttgcaa tttcgtcgcg gttcccgtca cgactgtcgt
      781 cgaagcgccg ctgatgccgc tggcccatcg tcactatcgg catgcgcgcg cctatcggtc
      841 atgcctttgc cggcggcgcc acgccgtcga tccgccgacg ccatgaccta ggcgcgcgtc
      901 gccggctgcg gcgccgcctt cacgcaatct tgccttgacg cgctccgccg cgcccgctgt
      961 catccgcgtc catcgggcag agcctcttca gggagggctt gatgcgcgtc acgcgtcgca
     1021 gggccgccgc tcttctctcg tccgcctcgg tcctcagctt gatcggcggg aaggcgcgcg
     1081 ccggcgatcc ggtcgacgcc gctatcgtcc tcgccgccga cgtctcgcgc agcatcaatg
     1141 acgaggagtt cggcctgcag cggcagggct atgccgtcgc gatcacgaac gcgcgcgtga
     1201 tggaggcgat ccggtccggc ccgcgcgccg cgatcgccct ctgcttcctg gaatgggcgg
     1261 gcgaaggcga gcagagggtc ctggtcgagt gggcgacgat caagaccgac gccgacgcga
     1321 gccggttcgc cgcggcccta agcgccgcgc cgcgcgcctt tgtcggccgc acggcgatcg
     1381 gaagcgccat cgacttttcg atgggcgtgc tcggcgagag cggcattgcg gccgaccggc
     1441 gcgtcatcga tgtttccggc gacgggacga gcaatcaggg ccgcttcgtc accgaagcgc
     1501 gcgacgccgc cgtcaaaacc ggcgtcgtca tcaacggcct cgccatcttc aaccgccgcg
     1561 ccgcggcgat gggcggctat cttgcgctgc ataccaaccc ttcgggcggg ctggcgaaat
     1621 attaccacga caacgtgatc ggcggccccg gcgccttcgt cctgccgatc gacgatttca
     1681 agagtttcgg cgaggcgatg atccgcaagc tggtcagcga gatcgccggc gcgtatcccc
     1741 ctcgctccgc gcagcgggga gagggtggcg gcgaagccgc cgggtgaggg gctgcgcgga
     1801 ggattgctga ttagcgtctt ataacacgta cctttcgaat cctgcgcagc ccctcgtcag
     1861 gcccttcggg ccaccttctc cccgcttcac gcagggctgt ccggggaaag tctgaggtta
     1921 tggcgcgtct tggacgtaat ctttcagcgc cgcggcgcca cgccggccga tcgccgtcaa
     1981 cccgccatag cgccggcaag ctacgtgcaa gatcatatcc gcttgtgc
//
LOCUS       DATGJW010000534         5455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1239145, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000534 DATGJW010000000
VERSION     DATGJW010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5455
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239145"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..686)
                     /gene="gpt"
                     /locus_tag="VKU03_16215"
     CDS             complement(183..686)
                     /gene="gpt"
                     /locus_tag="VKU03_16215"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013652367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HLJ72861.1"
                     /translation="MSEAQQKKAFPVSWDQFHRDARALAWRLTSAGPFEAIVCVTRGG
                     LVPAAVVARELGTRIIETVCVASYHDYKTQGGLQVLKPISPDVVKLGDGKGVLIVDDL
                     VDTGATAKVVREMLPKAHFAAVYAKPLGRPMVDTFITEVSQDTWIYFPWDMGLAFQPP
                     IAKGSEG"
     gene            complement(888..1640)
                     /locus_tag="VKU03_16220"
     CDS             complement(888..1640)
                     /locus_tag="VKU03_16220"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012779226.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-binding protein"
                     /protein_id="HLJ72862.1"
                     /translation="MNETYKTVTAAILVIGDEILSGRTKDRNIGYIAEYLTNMGVELR
                     EARVVPDVEEEIVAALNALRARYTYVFTTGGIGPTHDDITADAVAKAFDVAIGEDPRA
                     IALMMERYKPGELTEARRRMARIPEGADLVANPISKAPGFRIGNVIVMAGVPSVMQAM
                     LDDAAKGLETGQKVLAETIEAGNIAEGRYGGPLGKIAAAHPGVSIGSYPAYIEGKFRN
                     QIVIRAKEAALIAAARIEVEAMLAALGEEAPR"
     gene            complement(1647..2513)
                     /locus_tag="VKU03_16225"
     CDS             complement(1647..2513)
                     /locus_tag="VKU03_16225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019904688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HLJ72863.1"
                     /translation="MSSALAQNSAAADPAPFGHYAPTGLCAAALRITRRCSDRWLAKR
                     LAFFLRSNAIKWLGGRPIDVETLGVRMRLRPYNNVAEKRLLFTPQYFDRRERALIAER
                     MGEGSIFIDAGASVGGYALSIAAAAPRARILAIEPLPEVFERLVYNIDQNALANVKAL
                     SCALADRDGEVTLFVNEDNRGETSLRVVSEEASSIRLRVPAKSLLSVLRGEGYERVDV
                     IKLDIEGAEDLALDTFFRDAPASLWPRLIVMEFNLLPPEGGLEERLRGLGYREILRTA
                     ENVAYERDQPMA"
     gene            complement(2587..3270)
                     /locus_tag="VKU03_16230"
     CDS             complement(2587..3270)
                     /locus_tag="VKU03_16230"
                     /EC_number="4.2.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018271394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HLJ72864.1"
                     /translation="MEIGFLVFPKVQQLDLTGPYEVFASWPGARVRLVAKSRAPIASA
                     TGLVLTPDLTFEDCPQLDVICVPGGVGINPLLNDEETLAFLRRQASGARFVTSVCTGA
                     LVLGAAGLLRGKRATTHWASHNFLSAFGAIPVEARVVRDGDLMTGGGVTAGIDFALAL
                     VAELAGREAAEAIQLNFEYAPAPPFDSGRPDTAPAEIVAAVRKLSATVRAEREVLVAE
                     AARKLSIAA"
     gene            3331..4326
                     /locus_tag="VKU03_16235"
     CDS             3331..4326
                     /locus_tag="VKU03_16235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015928529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ72865.1"
                     /translation="MTSIQHFQPNRRAVEFVAFGDFQLLDVAGPSQVFATANDLARLA
                     RQPAPYTLQVVAGSDGLIASSSGMTLGAAALPAPGAPLDTLIVSGGYGVDKACKDEAL
                     LAWLKLRAAHARRLCSVCSGALILAAAGLLTGRRVATHWGRCAQLQRQYPEIKVENDP
                     IFVRDGHVWTSAGVTAGVDLALALVEEDLGRATALAVARQLVVFLKRPGGQAQFSAAL
                     ELQAADERFETLNAWISENLNADLSVPRLAEKAGMSERNFARRYKQATGVSPARAIER
                     LRVEAAQRRLCETREPIKRVAARCGFGGEETMRRSFQRIACIGPQDFRARFSREA"
     gene            4410..5237
                     /gene="map"
                     /locus_tag="VKU03_16240"
     CDS             4410..5237
                     /gene="map"
                     /locus_tag="VKU03_16240"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014891178.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HLJ72866.1"
                     /translation="MTFVDSAALHGRKSGHIKLHGPEAFAAMRVAGRLTAEALDLLAD
                     NVRPGVTTAALDKLCFEFAMDHHAYPAPLYYRGYRKAICTSINHVVCHGLPDDKPLRD
                     GDILNIDVTLIVDGWHGDSSRMYVAGEAPRRAQRLIDITYESLMRGIAAVKPGATTGD
                     IGAAIQTYAEGERCSVVRDFCGHGLGRLFHDEPNILHYGRSGEGLPLRPGMLFTIEPM
                     INLGRPQVKVLSDGWTAVTRDRSLSAQFEHTVGVTETGAEVFTYSPNGLDKPPHTAA"
BASE COUNT          987 a         1831 c         1679 g          958 t
ORIGIN      
        1 ttaagtccgt tgcgtctacc agtttcgcca cgcccgcatg ctgccggttc tagcggccaa
       61 acgccaaaga gccaatgaaa aatcttgggg ttaatatcgt gcgggtcggc cataaattct
      121 tcgcctgcgc ccctctgctg cgagcggtgt gtttagacgc ggcgcttcgt ggcgaaatag
      181 ccctaccctt cgctcccctt ggcgatcggc ggctggaacg ccagccccat gtcccaggga
      241 aagtaaatcc aagtatcctg cgagacttcc gtgatgaacg tgtcgaccat cggccggccg
      301 aggggtttgg cgtagacggc ggcgaaatgg gccttgggca gcatttcgcg caccactttc
      361 gcggtcgcgc cggtgtcgac gagatcgtcg acgatcagca cgcctttccc atcgccgagt
      421 ttgacgacgt cgggcgagat cggcttcagg acctgcaggc cgccctgggt tttatagtca
      481 tgataggagg cgacgcagac cgtctcgatg atccttgtgc cgagttcgcg ggcaacgacc
      541 gccgccggca ccaggccgcc gcgggtgacg cacacgatcg cctcgaaagg cccggcgctg
      601 gtcaggcgcc aggcgagcgc ccgcgcgtcg cggtggaatt gatcccagga gacgggaaag
      661 gcctttttct gctgggcttc tgacatggct catccatcgc gggcttgggc agctttgata
      721 gcaagccaac gccgccctcg cgagggtcga ggcctgtgac tcacaggatt tacgcctcct
      781 cccctgcgaa gcgggggagg gggaccatgc gaagcatggt ggagggggta cggcggttgc
      841 ggataagacc taaaggaaac cgccgctacc gcggcgcctc tccgccgcta ccgcggcgcc
      901 tcttcgccaa gcgccgccag catcgcctcc acctcgatcc gcgccgccgc gatgagcgcc
      961 gcctctttgg cccggatcac gatctgattt cggaacttgc cctcgatata ggccgggtaa
     1021 gaaccgatcg aaacccccgg atgcgccgcc gcgatcttgc ccaagggtcc gccgtagcgc
     1081 ccttccgcga tattgccggc ttcgatcgtc tcggcgagga ctttctgccc cgtctccagc
     1141 cctttggccg cgtcgtccag catcgcctgc atcacgctcg gcacgccggc catgacgatg
     1201 acattgccga tccgaaagcc cggcgctttc gatatcggat tggcgacgag atcggcgcct
     1261 tcggggatgc gcgccatgcg ccttcgcgcc tcggtcagtt cgccgggctt ataacgctcc
     1321 atcatcaaag cgatggcgcg cgggtcttcg ccgatcgcca cgtcgaaagc cttggcgacg
     1381 gcgtcagcgg tgatgtcgtc atgcgtcggg ccaatgccgc cggtggtgaa cacgtacgta
     1441 tagcgcgccc gcagcgcatt cagcgccgcg acgatctcct cttccacatc aggcacgacg
     1501 cgcgcctcgc gcaattcaac gcccatattg gtgagatatt cggcgatata gccgatattg
     1561 cgatccttgg ttcgtccaga gaggatttca tcgccgatga ccaggatcgc ggccgtgacg
     1621 gttttgtacg tttcgttcat ttggttttac gccatgggtt gatcgcgttc ataagcgaca
     1681 ttttccgccg tgcgcaggat ttcgcgatag ccgagaccgc gcaagcgttc ctcgagcccg
     1741 ccctcgggcg gcagaagatt gaactccatc acaatcaagc gaggccagag cgaagccggc
     1801 gcgtcgcgga aaaaggtgtc gagcgccaga tcctcggcgc cctcgatatc gagcttgatg
     1861 acgtcgaccc gctcatagcc ctcgccgcgc aataccgaga gaagactttt cgccggcacg
     1921 cgcaggcgta tcgaactcgc ttcctcactg acgacgcgga gcgacgtctc gccgcgattg
     1981 tcctcgttga cgaagagcgt gacttcgccg tcgcgatcgg caagcgcgca ggacaaagcc
     2041 ttcacattgg cgagcgcatt ctgatcgata ttgtagacga gccgttcgaa cacttccggc
     2101 aaaggctcga tcgccaggat gcgcgcccgc ggcgcggcgg cggcgatcga caaagcgtag
     2161 ccgccgacgc tggcgcccgc gtcgatgaag atggagccct cgcccatccg ctcggcgatc
     2221 agcgcgcgct cgcggcggtc gaaatattgc ggcgtgaaca gaaggcgctt ttccgcgaca
     2281 ttattgtagg gcctgagccg catgcgcacg ccgagcgttt cgacatcgat cggtcgcccg
     2341 cccagccatt tgatcgcatt cgagcgcaaa aagaacgcca ggcgcttggc gagccaccgg
     2401 tcggagcagc ggcgggtaat gcgcagcgcc gccgcgcaca ggccggtcgg cgcataatgg
     2461 ccaaaaggcg ctggatcggc agcggcggaa ttctgggcga gggccgacga catggggcgt
     2521 cggcccatac cacgcacagc gacggcaagg ccagcgccgc gcgtgcggtt ttgcaacgtc
     2581 ttttcgtcag gcggcgatgc tgagctttcg cgccgcttcg gcgaccagaa cttcgcgctc
     2641 cgccctcacc gttgcgctaa gcttgcgaac agcagcgaca atctcggccg gcgccgtgtc
     2701 gggcctgccg gaatcgaaag gcggcgccgg cgcatattcg aaattgagct ggatcgcttc
     2761 ggccgcctca cggcccgcaa gctcggcgac gagcgccagc gcgaagtcga tccccgccgt
     2821 gacgccgccg ccggtcatca gatcgccgtc ccgcacgacg cgcgcctcga cgggaatagc
     2881 gccgaaagcg gagaggaaat tgtgcgaggc ccaatgcgtc gtcgcccttt tgccgcggag
     2941 caagccggcg gcgccgagaa ccaaggcgcc cgtacagacc gaggtgacga aacgcgcgcc
     3001 cgacgcctgc cgccgcaaaa aggcgagcgt ctcctcgtca ttgagaagcg gattgatccc
     3061 aacgccgccg ggaacgcaga tgacgtcaag ctgcggacaa tcttcgaaag tcaggtccgg
     3121 cgtcagcaca agcccggtcg ccgaagcgat cggcgcgcgg ctcttggcga ccagccgaac
     3181 ccgcgcaccc ggccaggagg cgaagacttc gtaaggccct gtcagatcga gctgctggac
     3241 cttggggaaa acaagaaagc cgatttccat tgcgtgacgc tcctaaatct gttgccgccg
     3301 gcgaccttaa tcggctatgg attggctgaa atgacaagca tccaacattt tcagccaaac
     3361 cgacgcgccg tcgaattcgt cgcctttggc gattttcaat tgctcgatgt cgccggcccc
     3421 tcccaggtgt tcgccacggc gaacgatctc gcgcggctcg cgcgccagcc cgcgccttac
     3481 acgctgcaag tcgtcgccgg cagtgacggc ctcatcgcaa gttcgtcagg catgacactc
     3541 ggcgccgccg ccctgcccgc ccctggcgcg ccgctcgata cattgatcgt ctccggcggc
     3601 tatggcgtcg acaaagcctg caaggacgaa gccttgctgg cttggctgaa actgcgagcg
     3661 gcgcacgcgc gccgcctctg ctcggtgtgc tccggcgcgt tgatcctggc cgcggcgggc
     3721 ctgctcaccg gccggcgcgt cgccacccat tggggtcgct gcgcccagct tcagcggcaa
     3781 taccccgaga tcaaagtcga aaacgacccg attttcgttc gcgacgggca cgtctggaca
     3841 tccgccggcg tcaccgccgg cgtcgatctc gccttggcgc tggtcgagga agaccttggc
     3901 cgcgcgaccg ccctcgccgt cgcccgccag ctcgtggtct ttctcaaacg tcccggcggc
     3961 caggcgcaat tttcagccgc tctcgagctc caagccgccg acgaacgctt cgaaacgctg
     4021 aacgcctgga tcagcgaaaa cctcaacgcc gatctctcgg tgccgcgcct cgccgagaag
     4081 gccggcatga gcgagcgcaa tttcgccagg cgctacaagc aggcgacggg cgtgagcccg
     4141 gcgcgggcga tcgaacgcct gagggtcgaa gcggcgcagc ggcggctttg cgagacgcgc
     4201 gagcccatca agcgcgtggc ggcccgctgc ggttttggcg gcgaagagac gatgcggcgc
     4261 agcttccagc gcatcgcctg tatcggaccg caggatttcc gcgcccgctt ctcacgcgaa
     4321 gcgtgaaacg gcgccgcttg cgcgcgccgc cgaagacctt acattaggtc gataatccac
     4381 tgggcttgct gggctttacg cgttacgcca tgacattcgt cgattctgcc gcgctgcacg
     4441 gccgcaagag cggccatatc aaactgcacg gacccgaggc tttcgcggcc atgcgcgtcg
     4501 ccggccgtct gactgccgaa gcgcttgatc tgctcgccga taatgtgaga cccggcgtaa
     4561 ccaccgccgc gctcgacaag ctctgcttcg aatttgcgat ggaccaccac gcctatccgg
     4621 ccccgctcta ctaccgcggc tatcgcaagg cgatttgtac ctcgatcaat cacgtcgtct
     4681 gtcacggcct gcccgacgac aagccgcttc gcgatggcga tattctcaac atcgacgtca
     4741 cgttgatcgt cgacggctgg cacggcgatt cgagccggat gtacgttgcg ggcgaagcgc
     4801 cccgccgcgc gcaacgcctg atcgacatca cttatgaatc cttgatgcgc ggcatcgccg
     4861 ccgtgaagcc cggcgcgacg acaggcgata tcggcgcggc gatccagact tacgccgaag
     4921 gcgagcgctg ctcggtcgtg cgcgacttct gcggccacgg cctcggccgg ctcttccacg
     4981 acgagccgaa tattctccac tacggtcgct ccggcgaagg cctgccgtta cggcccggca
     5041 tgctcttcac gatcgagccg atgatcaatc tcggccggcc gcaggtgaaa gtcctgtccg
     5101 acggatggac cgccgtgacg cgcgaccgtt cgctctccgc gcagttcgaa catacggtcg
     5161 gggtcaccga gaccggcgcc gaagtcttta cctattcgcc gaatggcctg gacaaaccgc
     5221 cccataccgc cgcgtaaatt gaagcccatt ctagccatca gatttcgacg caaattaatt
     5281 tactggaagc ggaaatgtac gaaccgccct atggcgcatg acggccgagg ctgagccgcc
     5341 gccccactat ctcggccatc gcgagagact gaaaagccgg ctgcgcgacg ccggccctga
     5401 agcgatcgcc gattacgaac ttgtcgagct cgtacttttt cgcgccattc cccgc
//
LOCUS       DATGJW010000535         2655 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_205550, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000535 DATGJW010000000
VERSION     DATGJW010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2655)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2655)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2655
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_205550"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(26..1246)
                     /locus_tag="VKU03_16245"
     CDS             complement(26..1246)
                     /locus_tag="VKU03_16245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019859398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HLJ72867.1"
                     /translation="MDSLITAAARALAIGDPLEALNKVALREDAPALALRGIAMAQLG
                     ELARAKALVKRAARAFGPREAVARARCAVVEAEIALASRDLASSAKALDAAAAILERR
                     GDRANAAHARLLEIRRLILIGRLDEAEGRLASIDLTALTPALRAIHELALAGIAMRRV
                     RAHAAQAALGRAEEAARWARIPALAAEVEQALAVLDTPVARRIARGQERPLRLEDVEA
                     LFASKALVVDACRNLVREKDKLAPLARRPVLFALVRALGEAWPDDVSRDVLLTRAFGA
                     KHADESHRARLRVEIGRLRRLLRAVARVNATKRGFALEPLSAHEVVVLARPVEERHAA
                     VLAFLADGESWSGSALALALALSQRTVQRALKELVTTGKAQSYGRGRARRWMTPPLPG
                     FATILLLTAPPPGG"
     gene            1333..2097
                     /locus_tag="VKU03_16250"
     CDS             1333..2097
                     /locus_tag="VKU03_16250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015007182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DUF899 domain-containing protein"
                     /protein_id="HLJ72868.1"
                     /translation="MITHTVGTREQWLAARLELLDAEKALTRQSDDLAQKRQELPWVR
                     VDKDYRFDTDEGSASLADLFRGRSQLLVYHFMFGPDYKAGCPSCSMIADGFDGFAVHL
                     ANHDVTLMAVSRAPLAKLQTYKQRMGWTFPWVSSHGGDFNFDFNVSYTEAQEKEGPVE
                     YNYARGAHAMDITPVPPPVAQFAATCGVDASTYRRDRPGMSAFVREDGVIYHTYSTYA
                     RGLDGLWGAYQWLDRAPKGRNEQGIWWRRHDEYARA"
     gene            2105..2347
                     /locus_tag="VKU03_16255"
     CDS             2105..2347
                     /locus_tag="VKU03_16255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234731.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72869.1"
                     /translation="MRQKRAGSGQIASGVARWLGLAGAPTFAVMALLTYGLGGDAGMM
                     CATAHGLSPLSGMAPMYLLMSAFHSGPWLRLVSGGK"
BASE COUNT          461 a          892 c          853 g          449 t
ORIGIN      
        1 ctttggatgt cttcattctt ccgtcctagc cgcccggcgg cggcgccgtg agtaacaaga
       61 tcgtcgcgaa tcccggcaag ggcggcgtca tccagcgccg ggcgcggccg cggccatagg
      121 attgcgcctt gcctgtcgtc acaagctcct tgagcgcgcg ttgcacggtg cgctgactca
      181 gcgccaacgc cagcgccagc gccgagcccg accaggattc gccatcggcc agaaaagcga
      241 gcacggccgc atgccgctcc tccaccggcc gcgccagcac cacgacttcg tgcgcgctca
      301 gcggctccag cgcaaagccg cgtttcgtcg cgtttacccg tgcgaccgcc cgcagcaggc
      361 ggcggagccg cccgatctcg acccgcagcc gggcgcgatg agattcgtcg gcgtgcttgg
      421 cgccaaaggc gcgggtaagc aagacgtccc gagatacgtc gtccggccac gcttcgccaa
      481 gcgcacgtac aagcgcgaac agcaccggac gccgcgcgag cggcgcgagc ttatccttct
      541 cacgtaccag gttgcgacag gcgtcgacga caagcgcctt cgaggcgaac agcgcctcga
      601 catcctccag ccggagaggg cgctcttggc cgcgcgcgat ccgccgcgcc accggcgtat
      661 cgagaaccgc gagcgcctgt tcgacctccg ccgccaacgc cggaatgcgc gcccagcgcg
      721 cggcttcttc ggcgcggcca agcgccgcct gggcggcgtg cgcgcggacg cgccgcatgg
      781 cgattccagc gagcgcgagt tcatgaatgg cgcgcagcgc cggcgtcagc gcggtaagat
      841 cgatcgacgc gagcctgccc tcggcctcgt cgagccgtcc gataaggatc aggcgccgga
      901 tctcgagaag gcgcgcatgg gccgcgttcg ccctgtcgcc gcgcctttcc agaatcgccg
      961 cggccgcatc gagcgccttg gcggacgagg cgagatcacg cgaagcaagc gcgatctccg
     1021 cttcgacgac agcgcatcgc gcgcgcgcca ccgcctctct tgggccgaag gcgcgcgccg
     1081 cgcgtttcac caaagctttg gcgcgggcaa gctcgccgag ctgagccatg gcgatgccgc
     1141 gcagcgcgag cgccggcgcg tcttcgcgaa gcgcaacctt gttcagcgcc tcgagcggat
     1201 cgccgatggc gagcgcccgc gccgccgccg tgatcaagga atccatcgaa atcccgtcac
     1261 acttgtcact cccagggccg gcaagttcag tcctagtcta agtcccagca atattcataa
     1321 cggagaatga cgatgatcac gcatacggtc gggactcggg aacaatggtt agcggcgcgg
     1381 ctcgaattgc tcgatgccga aaaggcgctg actcgacaga gcgacgacct cgcccagaaa
     1441 cgccaagaac tgccttgggt ccgcgttgat aaggactatc gtttcgacac cgacgaagga
     1501 agcgcctcgc tcgcggacct tttccggggc cgttcgcagc ttctcgtcta tcatttcatg
     1561 ttcggaccgg actacaaggc gggctgtccg tcttgctcga tgatcgccga cggttttgac
     1621 gggttcgccg tccatctcgc caatcacgat gtcactttga tggcggtgtc gcgggcgccg
     1681 ctggccaagc tccagacgta caagcagcgc atgggttgga cttttccctg ggtctcgtcg
     1741 catggcggcg acttcaattt cgatttcaat gtctcctata ccgaggcgca ggagaaggaa
     1801 ggtcccgtcg aatataatta tgcgcgcggc gcccatgcaa tggatatcac gccggttccg
     1861 ccgcctgtgg cgcagtttgc cgcgacttgc ggcgtcgacg cctcgaccta tcgtcgcgac
     1921 cggcccggca tgagcgcctt cgtgcgggag gatggcgtca tatatcatac ctactcgacc
     1981 tatgcgcgcg gcctggacgg cctctggggc gcctatcaat ggctcgatcg cgcgccgaaa
     2041 ggtcgtaacg agcaaggcat ctggtggcgc cgtcacgacg agtacgctcg tgcttgaggc
     2101 gtcgatacgc cagaagcgcg ccgggagcgg tcagatcgct tccggcgtgg ccagatggct
     2161 cggccttgcc ggcgcgccga ccttcgccgt catggcgctt ctcacctatg ggcttggcgg
     2221 cgacgccggc atgatgtgcg cgacggcgca cgggctgtcg cccctcagcg gcatggcgcc
     2281 aatgtatttg ctgatgagcg ccttccattc agggccctgg ctgagattgg tgtccggcgg
     2341 caaataaagc cgcggcgccg agacatgagc acgacgccag ggcctcctct ccccgtgcag
     2401 cgaagcaaaa cggggagagg taaggccggg tgtttaagta cgaccgtcgc agccggacga
     2461 cgttccgtac ctccatcaga gagtgcgcgt tctcccgctt cggcttcagc ggcaattgtc
     2521 gacatgtcgg tacacgatat cgtcaaggtg acgcaataac tgacgcgcgc cgaggatagg
     2581 cgcacaattc ctcatctcga tgctttgcgg ccgtagtgga ggatcaccgc gccggtgtcc
     2641 aacggcgtga cggcg
//
LOCUS       DATGJW010000536         3598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1452114, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000536 DATGJW010000000
VERSION     DATGJW010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 3598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3598
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1452114"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..545
                     /locus_tag="VKU03_16260"
     CDS             <1..545
                     /locus_tag="VKU03_16260"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HLJ72870.1"
                     /translation="PGYGDPGAIAQGGEAQRSDAGASASVKSARTVDRRGGAPYHLTN
                     MLNEAPSSRAKSDPLSATFAALADPTRRAILAALAGGEQTVGALALPFPISGPAISRH
                     LRVLAEAGLIERKASARWRICSLRADAMRGAHQWLSDYQRYWEASLDRLAELVEAPER
                     KAAAQPKKTKDSKSERRRGK"
     gene            542..1654
                     /locus_tag="VKU03_16265"
     CDS             542..1654
                     /locus_tag="VKU03_16265"
                     /inference="COORDINATES: protein motif:HMM:NF019928.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SRPBCC domain-containing protein"
                     /protein_id="HLJ72871.1"
                     /translation="MKEAATFHLEIERFIKAPREKVFAAFTQESLLKAWHCPRGMTVA
                     EASVDARVGGAYRIVMASRDGSRFVVVGAYQAIEPPDFIAYTWRAEGGPASDLTTLIE
                     VSLTAKDGGTALRMRHTGFPNAQASASHRQGWQSCFNRLSDLVDAEGSAGTLTLFGDP
                     RSSYCWTARLGLAEKGVKYRLAPHAPHTPEQRAVHPFGLVPALRDGETEIYETRAILS
                     YVDEAFDGPPLAPTGGVTARARNEQWISLINCYGYDAMVRRCLFQHMAESGPPLVAAI
                     AAKSRAGRPVGEVVAEALREIDMQLDLFEKGYGANDYLTGSSPATADLFLAPLIHYFG
                     QLPQGRELLQKYPAVRHGHDVMKARPSFVATLPQAD"
     gene            1858..2691
                     /locus_tag="VKU03_16270"
     CDS             1858..2691
                     /locus_tag="VKU03_16270"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003615720.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ScpA family protein"
                     /protein_id="HLJ72872.1"
                     /translation="MAGQLAFETELEVDRGEGEPSFMVDLDGFEGPLDLLLELARRQK
                     VDLARISVLALAEQYLAFIEQARKVRLELAADYLVMAAWLAYLKSRLLLPDPPKPEGP
                     GAAELAEALAQRLRRLEQIRRAAEALINRPRLGRDFFGRGSPESIAVARRPAYQATLY
                     DLLSAYAARRQTQALTRVRFKERVVWSLEEARQALNRLVGVAVDWTEFDGWLFAYCLD
                     PKMRRTARASSFSAALEFAREGVIELRQDRAFAPIWVRAKPADRSEPVTPLEQGENQN
                     G"
     gene            2684..3463
                     /gene="scpB"
                     /locus_tag="VKU03_16275"
     CDS             2684..3463
                     /gene="scpB"
                     /locus_tag="VKU03_16275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020175644.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SMC-Scp complex subunit ScpB"
                     /protein_id="HLJ72873.1"
                     /translation="MGEVARLFEEPIEDEAAAIEPEALRIAEALVFAAAEPLDEAAIA
                     SRLPEGSDVSAVMAELQRVYAGRGVNLVRVARKWTFRTAADLSWLLSREDKEQKKLSR
                     AAIETLAIIAYHQPVTRADIEEIRGVAVSKGALDVLMETGWVRMRGRRKAPGRPITYG
                     TTMDFLVHFGLESIGDLPGLDELKGAGLFDGRLPAGFGVPSPSDDPSLTADEDPLEES
                     AALVEAYGAQATDESAEAGQAAADEPQGEEKTEEGARAPRR"
BASE COUNT          616 a         1183 c         1197 g          602 t
ORIGIN      
        1 agcctggata tggcgatccc ggcgcgatcg cgcaaggcgg cgaggcgcag cgatccgacg
       61 ccggcgccag cgcttcagtc aaatccgcgc gaacggttga caggcgtggc ggcgccccct
      121 accatttaac caacatgtta aatgaagcgc catccagcag ggccaaaagc gatccgcttt
      181 cagcgacttt cgccgcgctg gccgatccga cacggcgtgc gatccttgcc gctttggccg
      241 gcggcgagca gacggttggc gcgctggcct tgccttttcc aatttccggg ccggcgattt
      301 cccgccattt gcgggttctg gccgaagccg gcctgatcga gcgcaaggcg agcgcgcgct
      361 ggcgcatctg ctcgctcagg gccgacgcga tgcgcggcgc ccatcaatgg ctcagcgatt
      421 accagcgcta ttgggaggcc agcctcgatc gactcgccga gctcgtcgaa gcgccggagc
      481 gaaaagcggc ggcgcagccc aagaagacga aggattcgaa atcggagcga aggagaggga
      541 aatgaaggaa gccgcgactt ttcatctcga gatcgagcgt ttcatcaagg cgccgcgcga
      601 aaaggtcttc gccgccttta cgcaggaatc gctcctgaaa gcttggcatt gtccgcgcgg
      661 catgaccgtg gcggaggcga gcgtcgatgc gcgggtcggc ggcgcctacc gcatcgttat
      721 ggcgagccgc gacggctcgc gcttcgtcgt cgtcggcgcc tatcaggcga tcgagccgcc
      781 ggatttcatc gcctatacct ggcgcgccga gggcggccca gcctccgatt tgacgacgct
      841 gatcgaagtc agcctgacgg caaaggacgg aggaacagcg ctgcggatgc gccataccgg
      901 cttcccgaac gcccaagcga gcgcttcgca tcgccaaggc tggcaatcgt gcttcaaccg
      961 gctcagcgat ctcgtcgacg ccgagggcag cgccggaacc ctgaccctat tcggcgaccc
     1021 gcgcagctcc tattgttgga cggcgcgtct gggcctcgcg gagaagggcg tcaaataccg
     1081 gctcgcgccg catgcgccgc atacgcccga gcagcgcgcg gtgcatccgt tcggactcgt
     1141 tccggcgctg cgcgacggcg agaccgaaat ttacgaaacg cgcgccatcc taagctatgt
     1201 cgacgaggcg ttcgacggcc cgccgctggc gccgaccggc ggcgtgacgg cgcgggcgcg
     1261 caacgagcaa tggatcagtt tgatcaattg ctatggctac gacgccatgg tgcggcggtg
     1321 tcttttccag cacatggcgg agagcgggcc gccgctcgtc gccgcaatcg ccgccaagag
     1381 ccgcgctggc cggccggtcg gagaggtcgt cgctgaggcg ctgcgcgaga tcgacatgca
     1441 actcgacctt ttcgagaaag gctatggcgc gaacgattat ctgaccggct cgtcgccggc
     1501 gacggcggat ctgttcctcg cgccgctgat ccattacttc ggccaattgc cacagggccg
     1561 ggagttgctt caaaaatacc ctgcggtgcg ccatggccat gacgtgatga aggcgcgacc
     1621 gagctttgtc gcgacgctgc cgcaagcgga ttgacccgag acaaatggtc taacctgagg
     1681 cggccggcat gaccccgcct cagcgttagc ggcccgccac catcaatttg ccgcgaatct
     1741 attgaaatag cagccgaatt ttttcacgcg cgccgccctg tggagtctag tcgggcggcg
     1801 cttgcgcatg gccgctggct cggccaaaca agcttaacgg ctgtttaagg gtgattcgtg
     1861 gccggccaat tggctttcga gacggaactg gaggtcgatc gcggcgaagg cgagccttcg
     1921 tttatggtcg atctcgatgg attcgagggt ccgctcgatt tgctgctcga attggcgcgg
     1981 cggcaaaagg ttgatctcgc ccgcatttcg gtcctcgccc tcgccgaaca atatctcgcg
     2041 ttcatcgagc aggcgcgcaa agtgcggctc gaattggcgg cggattatct cgtgatggcc
     2101 gcctggctcg cctatctcaa gtcgcgcctg cttctgcccg atccgcccaa gcccgagggg
     2161 cccggcgcgg ccgagctcgc cgaggccctg gcgcaaaggc tgcgccggct cgaacagatc
     2221 cgccgggcgg cggaagcgct catcaaccgg ccgcgcctgg gccgcgattt cttcggccgc
     2281 ggctcgccgg aatcgatcgc cgtcgcccgc cggccggcct atcaggcgac cctttacgat
     2341 ctgctctccg cctatgcggc gcgccgccag acccaagcgc tgacccgcgt ccgcttcaag
     2401 gagcgggtgg tctggtcact ggaggaggcg cgccaggcgc tgaaccggct ggtcggcgtc
     2461 gcggttgatt ggaccgagtt cgacggctgg ctcttcgcct attgcctcga cccgaaaatg
     2521 cggcggacag cccgcgcctc gtccttctcc gccgcgctcg aattcgcccg cgagggcgtc
     2581 atcgaattgc gacaggaccg cgctttcgcg ccgatctggg tgcgcgccaa gcctgccgac
     2641 cgttccgaac cagtgacccc actcgagcaa ggcgagaacc agaatgggtg aagtcgcgag
     2701 acttttcgaa gagccgatcg aggacgaggc tgcggcgatc gaaccggaag cgctgagaat
     2761 cgccgaagcc ctggtcttcg ccgcggccga gccgctcgac gaggcggcga tcgcctcccg
     2821 cctgcccgag gggagcgacg tctcggcggt gatggccgaa ttgcagcgcg tctatgccgg
     2881 gcgcggcgtc aatctcgtcc gcgtcgcccg caaatggacg tttcgcaccg ccgccgatct
     2941 ttcctggctg ctttcgcgcg aggacaagga gcagaaaaag ctctcccgcg ccgcgatcga
     3001 aacgctggcg atcatcgcct atcatcagcc ggtgacgcgc gccgatatcg aagaaatccg
     3061 cggcgtcgcc gtctcgaagg gagcgctcga cgtgctgatg gagaccggct gggtgcgcat
     3121 gcgcggccgg cgcaaggcgc cgggccgtcc gatcacctat ggtacgacta tggatttcct
     3181 tgtccatttc ggcctggagt caatcggcga cttgcccggc ctcgacgaat tgaagggcgc
     3241 cggccttttc gacggacgat tgccggccgg tttcggcgtg ccgtcgccga gcgacgatcc
     3301 gtcgctgacc gccgacgaag acccgctcga agagagcgcg gcgcttgtcg aagcctatgg
     3361 cgcgcaagcg acggacgagt ccgccgaggc gggccaagcc gccgccgacg agccgcaagg
     3421 cgaagagaag acggaggagg gcgcccgcgc tccgcggcga taagctcgtc gtcggcgcgg
     3481 ctcattcctg ttccgccgcg atgagcctgc ccagcaaagc cgcaagctga tcggctcgcc
     3541 gtcgttgcgc agctttgcgc cgacgtggcg ctggatggcg gcgcgatagg ccggccac
//
LOCUS       DATGJW010000537        11516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_103010, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000537 DATGJW010000000
VERSION     DATGJW010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 11516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11516
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103010"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(210..958)
                     /locus_tag="VKU03_16280"
                     /pseudo
     CDS             complement(210..958)
                     /locus_tag="VKU03_16280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008551907.1"
                     /note="frameshifted; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
     gene            complement(1276..1656)
                     /locus_tag="VKU03_16285"
     CDS             complement(1276..1656)
                     /locus_tag="VKU03_16285"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RidA family protein"
                     /protein_id="HLJ72874.1"
                     /translation="MAKALGPRTNHQISFAVLAGGMLHATIIPVDMQSGAFVDGPMEV
                     QARQTLQNMQALLREAGGDMQHVVHLTMYLIDAKDMTALNAVYREFFVHEPYPARAAV
                     VVRELVGPPGLRLQIAAQACLRDD"
     gene            2174..3181
                     /locus_tag="VKU03_16290"
     CDS             2174..3181
                     /locus_tag="VKU03_16290"
                     /EC_number="2.7.7.65"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GGDEF domain-containing protein"
                     /protein_id="HLJ72875.1"
                     /translation="MKSLIKRTLQIAVILGIAVLGALCATKTSAVPEVEFYVLALIAL
                     GILGVGLGEIRDVVVAPGEPVFERLGSLKKGDPMAQQFESALTVIVKLIQAHLEENSH
                     YSDSLERANRDLPTLDKPEQIRAVVSLLIDENEKIQDKMSELSKNLESSREQVAKLRS
                     NLAEANELGLRDPLTSLGNRRFFDANLVKEIDEAHSENSDMCLVLADLDHFKKVNDRF
                     GHPVGDMVLKLFGELLSSNIKGRDIAARYGGEEFAIIFPNTKLRDAAQLIDDIRTQLE
                     TKQWMLSGTGQRIGMITASFGVACLRRGEDSEALLRRADAKLYEAKISGRNRVAVDTA
                     A"
     gene            3242..3682
                     /locus_tag="VKU03_16295"
     CDS             3242..3682
                     /locus_tag="VKU03_16295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72876.1"
                     /translation="MKNSLRQKPDRGFHRVLAEIQRTRREEDRDRRLRSPAAGAAFPF
                     VESETHEALDWRDESDFFVDDEPPPAESENLLSDSPEAIALELGLDRMLSEEELSRAR
                     RRFMWRNHPDRCSEAQRPLADRRVAVANMLIDRARAKLVGARRR"
     gene            complement(3697..4323)
                     /locus_tag="VKU03_16300"
     CDS             complement(3697..4323)
                     /locus_tag="VKU03_16300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72877.1"
                     /translation="MTAAMALGSAQQFEPSRKPKSLLKLTGDSALVGGGFALALCSLT
                     FAGYMIGDRDRQPYFPGEQYLAIFAKPSHGRGVQVAARPSPALARLTPPGDAGGVDLT
                     PTGSIRATLRADPPDGAGLPPPRYRLVSAKQDAAWVESALGFRQVRPGDVLSGFGRVA
                     AIERRNGGWALVADSGSILQLSDAAVLSPAEQSPDGRFARRLIFGRQP"
     gene            complement(4320..5645)
                     /gene="fliI"
                     /locus_tag="VKU03_16305"
     CDS             complement(4320..5645)
                     /gene="fliI"
                     /locus_tag="VKU03_16305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590675.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar protein export ATPase FliI"
                     /protein_id="HLJ72878.1"
                     /translation="MAWGRKNLEPVRVSGVVTEVAMSHYRVAGLSRFLKLGECVSLDI
                     GERVQYGEVVRIEDGHVTLKPFESRCDAGIGTRAYRAENLSLSPHPKWKGRAVDALGR
                     PIDGGDPLELGDRVMPLDAEPPAALRRARVKTPIKTGIRVIDLFSPLCAGQRIGVFAG
                     SGVGKSSLLAMLARAPEFDTVVIALVGERGREVREFLDDALASHRSEAIAVVSTGDES
                     PMMRRLAPKAAMTIAEYFRDRGDAVLLIIDSVTRFAHAARDVALAAGEPAVARGYAPS
                     VFSDLPRLLERAGPGEEGGGSITGFFSVLVDGDDHNDPVADSIRGTLDGHIVLDRAIA
                     DQGRYPAVNILSSISRLAHFGWTADQRALVARLRTLIARFEDTRDLRLMGGYTPGADP
                     TLDQAVGIVPKIYEAMTQTASAPPSGDAFQELAAALTAETKPEKPAKSG"
     gene            complement(5731..6456)
                     /gene="flgF"
                     /locus_tag="VKU03_16310"
     CDS             complement(5731..6456)
                     /gene="flgF"
                     /locus_tag="VKU03_16310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar basal-body rod protein FlgF"
                     /protein_id="HLJ72879.1"
                     /translation="MQSGLYVSLSGQVALERRLETIANNIANMNTAGFRADGVSFSAE
                     LAKAGEQQVAFASAGDTYITRGAGPLAKTDNPLDLAIQGDGWFGIQTPNGIAYTRDGR
                     MQMTETGALQTVNGYQVLDAGGAPIGLDPNAGPPTISADGMITQDGKQISAVGLFSIP
                     DDAKLTRTVNSGVIPDKPANPILDFTQNGVVQGSVEGSNVNPIMEMTKLITVTRNFDS
                     LSAAMSQSESSLQDAIKTLGGAT"
     gene            6606..7493
                     /gene="motA"
                     /locus_tag="VKU03_16315"
     CDS             6606..7493
                     /gene="motA"
                     /locus_tag="VKU03_16315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012385724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor stator protein MotA"
                     /protein_id="HLJ72880.1"
                     /translation="MNFIIGLVIAVGCILGGYAALGGHLQVLMQPYEFVIIAGSALGI
                     FVVANPLPTIKDCGKAILEAILDKAPKSRDFLDVLSVLHALMRELRGKARNEVEGHFD
                     NPKESEIFKAYPNLLANHDVTTFICDYCRLIIIGNARTHEIEALMDEEIETLTYDRQK
                     PVHALTQISDALPALGIIAAVLGVIHAMGALDQSPELLGGLIGAALVGTFGGIFLSYG
                     LVSPLAQKVKVVRRKQMRTFTLVKQTLLAFMNGAMPQVAVEFGRKTIPSSERPTIDTV
                     ENETISAGAPADAAIKEAA"
     gene            7497..8441
                     /locus_tag="VKU03_16320"
     CDS             7497..8441
                     /locus_tag="VKU03_16320"
                     /inference="COORDINATES: protein motif:HMM:NF014235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FliM/FliN family flagellar motor switch protein"
                     /protein_id="HLJ72881.1"
                     /translation="MTPDVASQSARRPQDPLIDQSNFSVERLPGLAVVFDQFAESLAQ
                     ALAPLCRGDAVFSVETIETSGLFETLSACRGLLAGMLYCPDLDARAAAIFDRPFVDAF
                     AHVVFGATQAPPARRAEKPERPFTRIESQLVEKASRAAAQALSAGFAGFAEAAFAFEG
                     QEPVADVQILGRRDMPVIAARLRFAAAGESGGLRVVIPQTTLLPIRPRLSKDPAGEAP
                     AIDPRWAKQLKAGVSSALIPINGVLEEFEMTLGDIAELAVGRVLDLRGDGSGRVRLES
                     GGHNLFWCKLVQGEGRYKLEIEEPIQPEEGMLDAALAN"
     gene            8496..8828
                     /gene="fliN"
                     /locus_tag="VKU03_16325"
     CDS             8496..8828
                     /gene="fliN"
                     /locus_tag="VKU03_16325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012589073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009288 - bacterial-type flagellum
                     [Evidence IEA];
                     GO_function: GO:0003774 - cytoskeletal motor activity
                     [Evidence IEA];
                     GO_process: GO:0001539 - cilium or flagellum-dependent
                     cell motility [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliN"
                     /protein_id="HLJ72882.1"
                     /translation="MTDSEEAYAATADKFDDPPKDVAVNYETILKIPVTVQVVLGTTT
                     MPVASLMKLGRGAVVALDQRVGEPVNVVVNGRIVARGEVVVVDEATSRFGVSLTEIVA
                     SRNAEKAA"
     gene            8841..9869
                     /locus_tag="VKU03_16330"
     CDS             8841..9869
                     /locus_tag="VKU03_16330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020177009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flagellar motor switch protein FliG"
                     /protein_id="HLJ72883.1"
                     /translation="MSAIASAEYPVQSRMLSGAEKAAALLLAMGRPLAARLLKRFDQL
                     ELRQITRAAAHLGAISSEALETLVEEFTENFTIGADLLGNAGEAEQLLTGALPPEEVA
                     DILSDVLGSSNSGLWEKLANASEASLAAYIEKEHPQTAAFILAKVNPASAAKVVALLP
                     RDLRNELLLRMIGPAEVSGEALRIVEAALQEDLLKNAGRAAGADSRARIADIVNRLDP
                     NAVEDVLQSIGEAQPKDAAILKKMLFTFDDLVNLSNRARALLFDSAPTETVVLALRGT
                     ETAFRDAVLSSMASRARRLVESELSNGASVPQRDIVKARRAIAELVLKMAQRGEIEIG
                     AAESEEAA"
     gene            9885..10979
                     /locus_tag="VKU03_16335"
     CDS             9885..10979
                     /locus_tag="VKU03_16335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011662592.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EscU/YscU/HrcU family type III secretion system
                     export apparatus switch protein"
                     /protein_id="HLJ72884.1"
                     /translation="MAEEQDKESQTEEPTEKRLADAIERGVVPMSREVGLLASLVSML
                     IVMAFLLRDAAGRLVGALVHFIDDPAGWRLDQGSDAIALAKLILLKALAFLAPIFALF
                     IVAGIVASAAQNAPRFVLDRIIPDFSRISIRNGFSRIYGLHGLTEFLKSVVKFSAVVA
                     IVAMVLNGQRILLVNAMFYEVGALPDRALNFAVRLVAASVAATLVVAAADIVWARLHW
                     LRGQKMSRQEVKEEIKQAEGDRLVKARLRSIRLDRSRKRMLAAVPKATMVVANPTHYA
                     VALRYVRSEGGAPIVVAKGQDLIALKIRAIAEEHEIPVIEDKPLARSLYDAVKVDAQI
                     PSEFYRAIAELIHFIHQKKSNWPIVRERRA"
     gene            10979..11422
                     /locus_tag="VKU03_16340"
     CDS             10979..11422
                     /locus_tag="VKU03_16340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72885.1"
                     /translation="MTVEHFRNFLRAQEKVVADGIVDVATELRLADAAELMVMIRNNQ
                     NANIADLVNSSTELFFKPGSLKYALSAGCSVGWDSAPTIALDMEFRNAPVCVFFRLML
                     GRSRAGVEVMDIIFDEKGLDQAEKTQRLAEAIADARLPVKRAKAS"
BASE COUNT         2056 a         3685 c         3733 g         2042 t
ORIGIN      
        1 caggcctgcg gcgttaaagt atttctcaac attgtgatct cggcgatgga aaccgagaag
       61 gacaggtccg gagcgtcccc gctgagggac atccgatggc cggcgcggag ggcgcacggc
      121 cggcaagcct aacccttgac gaaatccccg cagcggcgct gcggggactc gtgcgttgat
      181 tccgacctgg cgctgagcgg tccggcgcgt caccagccca gcggccagga ggcgaagcgc
      241 gaatcggccg gcttctcgac cgcggccacg ctcggcgccg aaaccgccgg aactcgatcg
      301 aggcaatagc cgagatagcg cttcgaatcg atcgggtcga agccgaggcg gtgcttgagc
      361 ttcttgcgca gcttggaaat gtggctctcg accacgttct cgtcgacgtc ttcgtcgaag
      421 agcccataga tcgaattgaa tatctgcgat ttggtgacgc ggcggccgcg attgctgacg
      481 atatattcca ggattctgcg ctcgcggcgc ggcagcggca gcggttcgcc cttcacttcg
      541 gggtcgcggc cgtcgaagaa cacgcaaaga tcgccgatcg tgacatgatc ggtctcgctc
      601 ttgttgcgcc ggttgatggc gccgacccgg gcgaggattt cccgcacatg gacgggcttg
      661 cgcagcacgt cgtcgacgcc ggcggcgaaa agctccaaag tctggtcgag cgactggcgc
      721 tcgttgaggc agatcaccgg cgcgcgcgag cgctcgcgga tgaggcgggg ataggattcg
      781 cgctgatcgc aatcgccgat caggaacgct tcgatggcgt tgacatcctc ctcgctgacc
      841 ttgccgatcc aatcgcgaaa ttctgtcggt cgatgccggc ggacgaaatt ccctcgcgat
      901 caaaactgga ggcgtagcct ccggttacga cgcttcgctc gtccacaata atatacatcg
      961 gcgctcctcg ttcgcagcaa gttcgtggaa aaacgcggga gactcggaca ctcggcacgg
     1021 cggccgcgat ccggcggcaa gcgggattgt cgagaggccg cgcgtcggca tggcgccgcg
     1081 attaccggac gcgcacgcgc gtccggccgg acgtttctcg acagcttgtc gtagaacccc
     1141 agccgcgcgg tcgagcctcg ggcctcgaaa gcgcccgcgc cggctcaacc gcgcgtcaaa
     1201 ttgataaggg cgatatggtt aacagcccct taatgcttat gtcagaaagg gttttaaaaa
     1261 gcggcgggtt tcggctcagt cgtctctgag acaggcttgc gcggcgattt gaaggcgcag
     1321 accgggcggg ccgaccaact cgcgcacgac caccgcggct cttgccgggt aaggctcgtg
     1381 gacgaagaac tcccgataga cggcgttcag cgccgtcatg tcctttgcat cgatgaggta
     1441 catggtcaaa tggacgacat gctgcatgtc gccgccggct tcgcgcagca aggcttgcat
     1501 gttctggagc gtctgccggg cttggacctc catagggccg tcgacgaagg cccccgactg
     1561 catgtcgacg ggtatgatcg tcgcgtgcag catgccgccg gcaagcacgg cgaagctgat
     1621 ttgatggttc gttcgcggtc ctagagcttt cgccatctgt ttcccgaccg gtccgcggcg
     1681 ttgctgtcac aaggcgtgag ccgcacgatt cgtctgatca atggtatgcg aatcgctccg
     1741 ccgaaagcta ccggccgttc gccggaagct tcccgtagcc tcgcgcgggg ctgcggcaat
     1801 ttgaccttgc cgagaaggcg cgactcagca aaaaagtcca aagggggagc ggtaaaaccg
     1861 ccggggcgag aaattgttta ctcgttttcg tttaatttaa agcattgccg cgccggtccg
     1921 ggttggattt ggctccggac ctggctcggc gcgcaaggct gttaggtcgc cggcgttcag
     1981 cgcctggcga cgggggcgaa acggcaatgg gtccatctcg aaagagccgg acgcaaacac
     2041 ggtcaggcgg cgttccggcg ccgcctcccg agccgcgcgc gcctttgatc gcccacaacg
     2101 ccgccgcagc tcgccggcgg ccggcgaatt tcgccatccc tggcttctct ttccgcgctg
     2161 ccgcccattc gccatgaaat ctctcatcaa gcggacgctg caaatcgccg tcattctggg
     2221 gatcgcggtt ctcggcgcgc tgtgcgcgac gaaaaccagc gccgtgcccg aagtcgaatt
     2281 ttacgtcttg gcgctgatcg ccttgggaat cctcggcgtc ggcctcggcg agatcaggga
     2341 cgtcgttgtc gctcccggcg agccggtctt cgaacgcctc ggttcgctca aaaagggcga
     2401 tccgatggcg cagcaattcg aaagcgccct cacggtgatc gtcaaactga ttcaggcgca
     2461 tcttgaagag aactcccatt attcggattc gctggagcgc gccaatcgcg atttgccgac
     2521 gcttgacaag cctgagcaaa tccgcgccgt cgtctcgctg ctgatcgacg agaatgaaaa
     2581 aatccaggac aagatgagcg aactgtcgaa gaacctggaa tcgtcgcggg agcaggtggc
     2641 gaagctgcgc tccaatcttg ccgaggccaa tgaattgggc ctgcgcgatc cgctgacctc
     2701 gctcggcaac cggcgcttct tcgacgccaa tctcgtcaag gagatcgatg aggcgcatag
     2761 cgagaacagc gatatgtgcc tggttctcgc cgacctcgac catttcaaga aggtcaatga
     2821 ccggttcggc catcccgtcg gcgacatggt gttgaagctg ttcggcgagc tcctatcgag
     2881 caatatcaag ggccgcgaca tcgcggcgcg gtacggcggc gaggaatttg cgatcatttt
     2941 cccgaatacg aagcttcgcg acgccgctca gctcatcgac gatatccgca cccagctcga
     3001 gaccaagcag tggatgctga gcggcaccgg tcagcgcatc ggcatgatca cggcctcttt
     3061 cggcgtcgcc tgcttgcggc gaggcgaaga ttccgaggcg ttgctccggc gcgccgacgc
     3121 caagctctat gaggccaaga tcagcggacg aaaccgcgtc gcggtcgata cggccgcctg
     3181 acaggctcct tttgagcatc gcccgtccgc ccccctccat tcggctttcg tctaacgggt
     3241 catgaaaaac tctctgcgac aaaagccgga ccgaggcttt catcgggtac tcgccgagat
     3301 ccagagaacg cggcgcgagg aggatcgcga ccggcgcttg cgcagcccgg cggccggcgc
     3361 ggcttttccg ttcgtcgaaa gcgaaacgca tgaggcgctg gattggcggg acgagtccga
     3421 cttctttgtc gatgacgagc cgccgcccgc cgagagcgaa aatctcttaa gcgatagtcc
     3481 ggaggcgatc gcgctcgaat tggggctcga tcgaatgtta agcgaagagg agctcagtcg
     3541 cgccaggcgc cgtttcatgt ggcgcaacca tcccgatcgc tgcagcgaag cgcaacgtcc
     3601 tctcgccgac cggcgggtcg cggtcgccaa tatgctgatc gatcgcgccc gcgccaagct
     3661 ggtcggcgcc cggcgccgct aggcccctcc ctggggctat ggctgtcgac cgaagatcag
     3721 ccgccgggcg aagcgtccgt ccgggctttg ctcggcgggc gacaaaacgg cggcgtcgct
     3781 gagttgaaga atcgagccgc tatcggcgac aagcgcccag ccgccattgc gccgctctat
     3841 cgccgcaacg cgaccgaatc ccgacaagac atcgcccggc ctcacctgcc ggaagccgag
     3901 cgcgctctcg acccaggcgg cgtcttgctt ggccgagacg aggcggtagc gcggcggcgg
     3961 cagcccggcg ccgtcgggcg gatcggcccg cagcgtcgcg cggatcgagc cggtcggcgt
     4021 caggtcgacg ccgcctgcat cgcccggcgg agtgagccgc gcgagagccg gcgacgggcg
     4081 cgcagcgacc tggacgccgc ggccatggct cggctttgcg aaaatcgcca gatattgctc
     4141 gcctggaaaa taaggctgcc ggtcgcgatc gccgatcata tagccggcaa aggtcagcga
     4201 gcagagcgcc agggcgaagc cgccgccgac caaggcgctg tcgccggtca gcttcaaaag
     4261 cgatttcggc tttcgcgaag gctcgaattg ctgcgcgctg cccaaggcca tggcggccgt
     4321 cacccgctct tggccggttt ttccggcttg gtctcggcgg tcagcgccgc ggcgagctcc
     4381 tggaaggcgt cgcccgatgg cggcgccgaa gcggtttggg tcatcgcctc gtagattttc
     4441 ggtacgatcc cgacggcttg atccaaggtc ggatcggcgc cgggcgtgta gccgcccatc
     4501 aggcggaggt cgcgggtatc ttcgaagcgc gcgatcaacg tgcgcagcct cgccacaagc
     4561 gcgcgctgat cggcggtcca gccgaaatgg gcgaggcgcg agatcgagga gagaatgttg
     4621 accgccggat agcggccctg atcggcgatg gcgcggtcga gcacgatatg gccgtccaga
     4681 gtgccgcgga tcgagtcggc gaccggatcg ttgtgatcgt cgccgtcgac gagcaccgag
     4741 aagaatccgg tgatcgagcc gccgccttct tcgccgggcc cggcgcgctc cagcaggcgc
     4801 ggcaaatcgc tgaagacgct cggcgcatag ccgcgcgcca cggccggttc gcccgccgcg
     4861 agcgccacgt cgcgcgccgc atgggcgaag cgcgtcaccg aatcgatgat cagcaggacg
     4921 gcgtcgccgc ggtcgcggaa atattcggcg atggtcatcg ccgcctttgg agcgagccgg
     4981 cgcatcatcg ggctttcgtc gccggtcgag accaccgcga tggcctcgct gcgatgggag
     5041 gcgagggcgt cgtcgagaaa ttcgcgcacc tcgcggccgc gctcgccgac cagggcgatc
     5101 accaccgtgt cgaattccgg cgctcgcgcc agcatcgcca gcagcgagga tttgccgacg
     5161 ccggagccgg cgaagacgcc gatgcgctgg ccggcgcaga gcggcgaaaa caaatcgatc
     5221 acgcggatgc cggtcttgat cggcgttttg acgcgcgccc ggcgcaaagc cgccggcggt
     5281 tcggcgtcga gcggcatgac gcgatcgcca agctccagcg gatcgccgcc gtcgatcggc
     5341 cggccgagcg catcgaccgc ccggcctttc catttcggat gaggagagag cgaaagattt
     5401 tccgcgcgat aggctctcgt tccaatgccg gcgtcgcaac gcgattcgaa cggctttagc
     5461 gtgacgtggc cgtcctcgat gcgcaccact tccccgtatt gaacgcgctc gccaatatcg
     5521 aggctgacgc attcgcccag cttcaggaag cgcgataggc cggcgacgcg ataatgcgac
     5581 atagccactt cggtgacgac gccgctcacg cggaccggtt caagattctt gcgtccccaa
     5641 gccatggcgc gctcgagccg gtcgagcgcg ttcaacggcc gctcttgccc cgtcacgctt
     5701 cgcccctctc tgtccggcca acccgggccg tcacgtcgcg ccgcctaggg ttttgatcgc
     5761 gtcctggaga gaggattccg attggctcat cgccgcgctg aggctgtcga aattgcgcgt
     5821 gaccgtgatg agcttcgtca tctccatgat cggattgacg ttcgatcctt cgaccgagcc
     5881 ctggacgacg ccattctggg tgaaatcgag aatcggattg gccggcttgt cggggatgac
     5941 gcctgaattg acggtgcgcg tcagcttggc gtcgtcgggg atcgagaaca ggccgaccgc
     6001 cgaaatctgc ttgccgtctt gggtgatcat gccgtcggcc gagatggtcg gcggcccggc
     6061 attagggtcc aggccgatcg gcgcgccgcc cgcgtcgagc acctgatagc cgttcacggt
     6121 ttgcagcgcg ccggtctctg tcatttgcat ccggccgtcg cgcgtatagg cgatcccgtt
     6181 cggcgtctgg atgccgaacc agccgtcgcc ttggatggcg aggtcgagcg gattgtcggt
     6241 cttggcgagc gggcctgcgc cgcgggtgat gtaggtgtcg ccggcggagg cgaaagcgac
     6301 ttgctgctcg ccggccttgg cgagctccgc cgagaaggaa accccgtccg cccggaagcc
     6361 ggccgtgttc atgttcgcga tattgttggc gatcgtctcc agccggcgct ccagcgcgac
     6421 ttggccggaa agagacacgt agagcccaga ctgcatgaac gccccatcgg cgtgcgcctt
     6481 ccggcgccgt tctcgccgtc gggcactgtt ccatcgccga cttgcgcgaa gctggcgtcg
     6541 cctcaaacag cttcgcgcaa gctcagcgcg gcaagtttgt taacgaaagg cgcgggagag
     6601 cgattttgaa ttttatcatc gggttggtca tcgccgtcgg ttgcattctc ggcggctacg
     6661 ccgcgcttgg cggccatttg caagtgttga tgcagcctta tgaattcgtg atcatcgcgg
     6721 gttcggcgct cggtattttc gtggtcgcca atccgctgcc gacgatcaag gattgcggca
     6781 aggcgatttt ggaggccatt ttggacaagg cgccgaaatc gcgcgacttt ctcgacgtgc
     6841 tcagcgtgct gcatgcgctg atgcgcgaat tgcgcggcaa ggcgcgcaac gaggtcgaag
     6901 gtcatttcga caatccgaag gaatcggaaa tcttcaaggc ctatcccaac ctcctcgcca
     6961 accacgacgt gacgacattc atctgcgact attgccgact catcatcatc ggcaacgcgc
     7021 gcacgcatga aatcgaagcg ctgatggacg aggagatcga gaccctgact tatgatcggc
     7081 agaagccggt ccacgccttg acccagatca gcgacgcgct gccggcgctt ggcatcatcg
     7141 ccgcggtgct cggcgtcatt cacgcaatgg gcgcgctcga tcaatcgccc gaactgcttg
     7201 gcgggctgat cggcgccgcg ctggtcggta cgtttggcgg catttttctt tcgtacggcc
     7261 tcgtttcgcc tttagcccag aaggtcaaag tggtgcgtcg caagcagatg cggacgttca
     7321 ccctggtcaa gcagacactg ctcgccttca tgaatggcgc catgccgcaa gtcgccgtcg
     7381 agttcggccg caagaccatc ccctccagtg agcgcccaac gatcgacacg gtggagaacg
     7441 agacgatctc cgccggcgcg cccgctgacg cagcgatcaa agaggcggcc tgatccatga
     7501 cgcctgacgt cgccagtcaa tcggcgcgac ggccgcaaga cccgctcatc gaccagtcca
     7561 atttctcggt cgagcgtctg ccgggcctcg ctgtggtgtt cgaccagttc gccgagagct
     7621 tggcgcaagc tctcgcgccg ctctgccggg gcgacgccgt cttttccgtc gagacgatcg
     7681 aaacgagcgg ccttttcgag accttgagcg catgccgcgg ccttttggcg ggaatgctct
     7741 attgccccga cctcgatgcg cgggcggcgg cgattttcga tcgtcccttc gtcgatgctt
     7801 tcgctcacgt cgtcttcggc gcgacgcagg cgccgccggc gcgccgggcg gaaaagccgg
     7861 agcgcccctt taccagaatc gagagccaac tggtcgagaa ggcgtcgcgc gccgccgcgc
     7921 aagctctgtc ggccggcttt gccgggttcg ccgaggccgc cttcgccttt gagggccagg
     7981 agccggtcgc cgacgtacaa attcttggcc gccgcgacat gccggtcatc gccgcccggc
     8041 tccgctttgc cgcggccggc gaaagcggcg gattgcgcgt cgtgattccg cagaccacgc
     8101 tgctgccgat tcggccgaga ctgtcgaagg atccggcggg cgaagcgccg gccatcgatc
     8161 cgcgctgggc caagcagctg aaggccggcg tgagctcggc tttgatcccg atcaacggcg
     8221 tcctcgaaga gttcgagatg actttgggcg acatcgccga actcgccgtc ggccgcgttc
     8281 tcgatttgcg cggcgacggc tcgggccgag tccggctcga aagcggcggc cacaatcttt
     8341 tctggtgcaa gctcgttcaa ggggaagggc ggtacaagct ggaaatcgag gagccgattc
     8401 agccggagga aggcatgctc gacgcggcgc tcgcgaatta gcggcttcgg ccggctttca
     8461 tccgcgaggg aagcgtcaaa ccaaggcgtt caatcatgac cgacagtgaa gaggcgtacg
     8521 ccgcgacggc ggataaattt gacgacccgc cgaaagacgt cgccgtcaac tacgagacga
     8581 ttctgaaaat accggtgacc gtccaagtgg tcttgggcac cacgaccatg cccgtggcga
     8641 gcctgatgaa gctcggccgc ggcgccgtgg tcgcgctcga tcagcgggtc ggcgagccgg
     8701 tcaatgtcgt ggtcaacggc cggatcgtcg cccgcggcga ggtggtggtc gtcgatgaag
     8761 cgacctcccg attcggcgtc tccctcaccg agatcgtcgc ctccaggaac gccgagaagg
     8821 cggcttaacc gggcaaagcc atgagcgcga tcgcgtccgc cgaatatccc gtccagtcgc
     8881 gcatgctgag cggcgcagag aaggcggctg cgctgctcct cgccatgggc aggccgctcg
     8941 ccgcgcgcct gctgaagcgc ttcgatcaat tggaattgcg ccagatcacc cgcgccgcgg
     9001 cgcatctcgg cgcgatctcc agcgaggcgc tcgaaacgct ggtcgaagag ttcaccgaga
     9061 acttcacgat cggcgccgat ctcctcggca atgccggcga ggccgagcaa ttgctgaccg
     9121 gcgccttgcc gccggaggaa gtcgccgaca ttctttccga cgtcctcggc agctcgaatt
     9181 cgggcctctg ggaaaagctc gccaacgcgt ccgaggcgtc ccttgccgcc tatatcgaga
     9241 aggagcatcc gcaaaccgca gccttcatcc tcgccaaggt gaatccggcc agcgccgcga
     9301 aagtcgtcgc gcttctgccg cgcgatctgc gcaacgaact gcttcttcgc atgatcgggc
     9361 cggcggaggt ttcgggcgaa gcgttgagaa tcgtcgaggc ggcgctgcag gaggacctcc
     9421 tgaagaacgc cggccgcgcc gccggcgccg atagccgggc gcgcatcgcc gatatcgtca
     9481 accggcttga ccccaacgca gtcgaagacg tgctgcagag catcggcgag gcgcagccga
     9541 aggacgccgc gatcctgaag aaaatgctct tcaccttcga cgacctggtc aacctctcca
     9601 atcgcgccag ggcgctgctc ttcgacagcg cgccgaccga aaccgtcgtc ctcgcgctcc
     9661 gcggcaccga aacggctttc cgcgatgcgg tcctttcctc gatggcttcg cgggcgcggc
     9721 gcctggtcga gagcgaactc tcgaacggcg cctccgtgcc gcagcgcgat atcgtcaagg
     9781 cgcgccgggc gatcgccgag ctcgtcctta aaatggcgca gcgcggcgag atcgagatcg
     9841 gcgccgccga aagcgaagaa gcggcgtagc ggggcggcgc cggcatggcc gaggagcaag
     9901 acaaggaaag ccagaccgag gagccgaccg aaaagcggct cgccgacgcc atcgagcgcg
     9961 gcgtcgtgcc gatgtcgcgg gaagtcggcc tgctcgcctc gctcgtttcg atgctgatcg
    10021 ttatggcgtt cctgctgcgc gacgcggccg ggcgcctggt cggcgcgctc gtccatttca
    10081 tcgacgatcc cgccggctgg cgcctcgacc aaggcagcga cgcgattgcg ctcgctaagc
    10141 tgatccttct caaggcgctc gcctttctgg cgccgatctt cgctctgttc atcgtcgccg
    10201 gcatcgtcgc ctccgccgcg cagaatgcgc cgcgcttcgt gctcgaccgc atcatccccg
    10261 atttctcgcg catctcgatc aggaacggct tttcgcgcat ctacggcctg cacggtttga
    10321 ccgagtttct caaaagcgtc gtcaagttca gcgccgtcgt cgcgattgtc gcgatggtcc
    10381 tgaacggaca gagaatcctg ctcgtcaacg cgatgttcta cgaagtcggc gctttgcccg
    10441 accgggcgtt gaatttcgcc gtccggctcg tcgcggccag tgtggcggcg accctcgtcg
    10501 tcgccgcggc cgacatcgtc tgggcgcggc ttcactggtt gcgcgggcag aagatgagcc
    10561 gccaggaggt caaggaggag atcaagcagg ccgagggcga tcgcctagtc aaggctcgcc
    10621 tgcgttcgat ccgcctcgac cgctcgcgaa agcgcatgct ggcggcggtg ccgaaagcaa
    10681 cgatggtcgt cgccaacccg acccattacg ccgtggcgct gagatatgtc cgtagcgaag
    10741 gcggcgctcc gatcgtcgtg gcgaagggac aagacctgat cgccctgaaa atccgcgcga
    10801 tcgccgagga gcacgagatt ccggtgatcg aggacaagcc tctcgcccgc tccctttacg
    10861 acgccgtcaa ggtcgacgcg caaatcccgt cggaattcta ccgcgcgatc gccgaactca
    10921 tccatttcat ccaccagaag aagagcaact ggcccatcgt cagagagagg agggcgtaat
    10981 gaccgtcgaa catttcagaa atttcttgcg tgcgcaggag aaggtcgtgg cggacggcat
    11041 cgtcgacgtc gcgacggagc tcaggctcgc cgacgccgcc gagctcatgg tgatgatccg
    11101 caacaaccag aacgccaata tcgccgatct ggtcaattcc tcgaccgagc tatttttcaa
    11161 acctggatcg ctgaaatatg cgctctccgc cggctgcagc gtcggctggg attcggcccc
    11221 gaccatcgcg ctggatatgg agttccggaa cgctccggtc tgcgtgttct tccggctgat
    11281 gctcgggcgc tcgcgcgccg gggtcgaagt catggatatc atcttcgacg aaaagggatt
    11341 ggaccaggcg gaaaagacgc agcgtctggc ggaggcgatc gccgacgccc gattgccggt
    11401 gaagcgcgcc aaagccagct gacgatcgat tggcaaatcg gccagggtct cggtgaaatg
    11461 cgaacgttcc gccttcgcac tttatccgca aacgtctaga atggagcggg cgaaag
//
LOCUS       DATGJW010000538         4251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1239892, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000538 DATGJW010000000
VERSION     DATGJW010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4251
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1239892"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1171)
                     /locus_tag="VKU03_16345"
     CDS             complement(<1..1171)
                     /locus_tag="VKU03_16345"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HLJ72886.1"
                     /translation="MLVGISFAANALANFLVGLLVAKFLGPTEYGRFALAFATAVLIN
                     AAGFDWLRLSAIRFYSERARLERPEVRATLDDCVATIAFIISLIVLAVAFSGVELPIS
                     TGLLAMAAATGVASGLYDYTTALARARFLDKTFTGIIIVKNLLGLLLTVGGAWWLHSA
                     ALALAGVTLSVAGALAAAGRPLLDRGAGPALGRWSMAKSFMAYGLPFVGSGILFQAIP
                     LINRTLVSSLFGFGETGQFSLANDLGVRVLAAIASTLDVLLFQLAVRADEAHGPARAR
                     AQLAENMTTVLAIVLPTAVGLWLILPSFEALIVPEAYRGPFAHYLALMLPGLAAYVML
                     QYTISPIFQIAKRPRPMIVVGLTACAADGLFILLLPRVDGFSFAVAQSGAQIIGLS"
     gene            complement(1366..2424)
                     /locus_tag="VKU03_16350"
     CDS             complement(1366..2424)
                     /locus_tag="VKU03_16350"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008126206.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="D-TA family PLP-dependent enzyme"
                     /protein_id="HLJ72887.1"
                     /translation="MSFDSLDTPCVLIDIDRVEANLKRAQAYADAHGLKLRPHIKTHK
                     LPRFALRQVELGAVGITCQKLGEAEVMADAGVKDIFLPYNILGARKLARLRALAERVN
                     LSVTADSRDTVEGLSRAFADAARPLPVLVECDTGMGRCGVQTPEEALALARAIAASPG
                     LVFGGLMTYPAAGKIEANAAWLDAARALLAREGLPPPVVSNGGTPDMWRADAVTAATE
                     HRPGTYIYLDRYQVAKGAGWLGDCALTVLATVVSLPTADRAVIDAGSKALTSDTLGMD
                     GFGLILGYPDAVIRSLSEEHGVVDLSASARKPAIGERLRIVPNHACVVSNLFDEVALI
                     SGDKVVDRVAVAARGRVD"
     gene            complement(2428..3309)
                     /locus_tag="VKU03_16355"
     CDS             complement(2428..3309)
                     /locus_tag="VKU03_16355"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MurR/RpiR family transcriptional regulator"
                     /protein_id="HLJ72888.1"
                     /translation="MIATLQRPAIDVVARLREIAASGSRSDQRLAAEILADPDFATHA
                     AIDRLAERAEVSEPTVTRFCRALGCSGLRDFKVRLAQAVATTGRYIRPAGREGRAPQL
                     IAAGAHSAIEAVCGAISAADLSRAADLIVRARMVRAYGSGGASSLAAVELENRLFRLG
                     VLAASHVDGEVQQMTAAAADPGMVVVAYSVSGEIRSVVEAVATARLYGAAAIAFTAPG
                     SHLAAAASLVFPFRIDEGTDIFRPSPARYALLAMTDMLAMSVAERIGAPAVERMRRIK
                     HHQALSNRDADRLPLGD"
     gene            complement(3306..>4251)
                     /locus_tag="VKU03_16360"
     CDS             complement(3306..>4251)
                     /locus_tag="VKU03_16360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003526380.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72889.1"
                     /translation="KSTLLKLIAGLDELTAGEIAIGGRVVNDRSPADRDIAMVFQSYA
                     LYPHMTVADNLGFHLKIARMPRQERDRRIARVAENLGIAQFLNRFPRDLSGGQRQRVA
                     MGRAIVRDPQVFLFDEPLSNLDAKLRVQMRTEIKALHQAVRTTVIYVTHDQIEAMTMA
                     DRIVVMHAGRIEQIGAPLELYDRPGNRFVAGFLGSPAMTFVPGQVERGDGPPLLRTKS
                     GLALPLGETSAASGQSVEIGIRPEHYRLCGEGEGFPFKALVIEPTGAETLLYGTIDGA
                     EARCVFRERIAAAPGDVLRLNVDAAHIHVFDQLTGARL"
BASE COUNT          766 a         1392 c         1473 g          620 t
ORIGIN      
        1 ttgaaaggcc gatgatttgg gcgccgcttt gcgcgacggc gaacgagaat ccgtcgacgc
       61 gcggcaaaag gagaatgaac agcccatccg cggcgcaggc cgtcaggccg acgacgatca
      121 tcggccgcgg ccgcttggcg atctggaaga tcggcgagat cgtatattgc agcatgacgt
      181 aggcggcgag gccgggcagc atcagcgcga gataatgcgc gaacgggccg cgataagcct
      241 ccggcacaat cagcgcctcg aagctcggca ggatcagcca cagcccgacc gccgtcggca
      301 ggacgatcgc cagcaccgtc gtcatattct cggcgagttg ggcgcgcgcg cgcgccggcc
      361 catgcgcctc gtcggcgcgc accgccaatt gaaacagcag cacgtccagg gtcgaggcga
      421 tcgccgccag aacccggacg ccaagatcgt tggcgagcga gaactgtccc gtctcgccga
      481 agccgaacag gctgctgacc agcgttcgat tgatcagcgg gatggcctgg aaaagaatcc
      541 cagagccgac gaacggcagg ccataggcca tgaagctctt ggccatgctc cagcgcccaa
      601 gcgccggtcc ggcgccgcgg tcgaggagcg gacggccggc ggcggcgagg gcgccggcga
      661 cgctcagcgt cacgccggcc aaggcgagcg cggcggagtg gagccaccag gcgccgccga
      721 ccgtcagcag caggccgagc aggttcttga cgataatgat cccggtaaag gtcttgtcca
      781 gaaagcgggc gcgggccagc gcggtcgtat agtcatagag gccgctggcg acgccagtcg
      841 ccgcggccat ggccagcagg ccggtcgaga tcggcaattc gacgcccgaa aaagcgaccg
      901 ccaggacgat gaggctgatg atgaaggcga tcgtggcgac gcaatcgtcg agcgtggcgc
      961 gcacttccgg ccgctcgagc ctggcgcgct ccgaatagaa gcggatggcc gaaagccgga
     1021 gccagtcgaa ccccgctgca ttgatcagca cggccgtcgc aaaggcgagg gcgaagcggc
     1081 catattcggt cgggccgaga aatttggcga ccagcaagcc gaccaggaaa ttggccagcg
     1141 cgttcgcggc aaacgagatt ccgactagca tgaaaccggc ctatggcggc aaacgccgcc
     1201 gcccggcgcg ggccgagcga cccttcattg ctagccgagg cgcgataaga aagtcttgac
     1261 gaagccatgg ccgagacggg gccggtcctt cgcgaccttc gctccgcctt tgtgtccttc
     1321 gtgtttcaga tttcacaaag gtcacgagcc gcgaaagccc tgcccctaat cgacccggcc
     1381 gcgcgcggcg acggcgaccc tgtccacgac cttatccccc gaaatcagcg ccacctcgtc
     1441 gaagaggttg gagacgacgc aggcgtgatt ggggacgatc cgcagcctct cgccgatcgc
     1501 cggctttcgc gccgaagcgg agagatcgac gacgccatgc tcctcgctca gtgagcggat
     1561 cacggcgtcg ggatagccga ggataagccc gaacccatcc atgccgagcg tgtcgctggt
     1621 cagcgccttg gagccggcgt cgatcacggc gcgatcggcg gtcggcaggc tcaccaccgt
     1681 ggcgagcacg gtcaacgcgc aatcgccgag ccagcccgcg cctttggcga cctggtagcg
     1741 atcgagatag atgtacgtgc cggggcgatg ctcggtcgcc gccgtcacgg catcggcgcg
     1801 ccacatatcg ggggtgccgc cattggagac gaccgggggc ggcaagcctt cgcgcgccag
     1861 caatgcgcgc gcggcgtcga gccaggcggc gttcgcctca atcttgcccg cggcggggta
     1921 cgtcatcaat ccgccgaaga cgagtcctgg cgaggcggcg atggcgcggg caagcgccaa
     1981 ggcttcttcc ggcgtctgta cgccgcagcg gcccatgccg gtgtcgcatt cgacgagcac
     2041 cggcagcggg cgggccgcgt cggcgaacgc tcgtgaaagc ccttccacag tgtcgcgact
     2101 gtcggcggtc acagagagat tgacgcgctc cgccaaagcg cgaagccggg cgagcttgcg
     2161 cgcgccgaga atattatagg gaaggaaaat atccttcacg ccggcatcgg ccatgacctc
     2221 ggcttcgccg agcttctggc aggtgattcc gaccgccccg agctcgactt ggcgcaaggc
     2281 gaagcgcggc agcttatgcg tcttgatatg cgggcgcagc ttgaggccat gcgcgtcggc
     2341 ataggcctgg gcgcgcttga gattggcctc gacccggtcg atgtcgatca ggacgcaagg
     2401 cgtatcgagg ctgtcgaagc tcattgatca atctccgagc ggcaatctgt cggcgtcgcg
     2461 attgctcagg gcctgatggt gcttgatgcg gcgcattcgc tcgaccgccg gcgcgccgat
     2521 ccgctcggcg accgacatgg ccagcatgtc ggtcatcgcc agcagcgcgt agcgcgccgg
     2581 cgacggccgg aatatgtcgg ttccctcatc gatgcggaag ggaaaaacca gcgaggccgc
     2641 agcggccaga tgcgagcccg gcgcggtgaa ggcgatcgcc gccgcgccgt aaaggcgcgc
     2701 ggtcgcgacc gcttcgacca ccgagcggat ttcgccggac acggaataag cgacgacgac
     2761 catgcccgga tcggccgccg ccgcggtcat ttgctgaacc tcgccgtcga catgggaggc
     2821 ggcgagcacg ccgagccgga acagccggtt ctcgagctcg accgcagcaa gcgacgaggc
     2881 gccgcccgat ccataggccc gcaccatccg cgcccggacg atgagatcgg ccgcgcgcga
     2941 gagatcggcg gcgctgatcg cgccgcacac cgcttcgatc gcgctgtggg cgccggcggc
     3001 gatgagttgc ggcgcccggc cctcgcggcc ggccggcctg atgtagcgcc cggtcgtcgc
     3061 caccgcctgg gccaggcgca ccttgaagtc gcgcaggccc gagcagccaa gcgcgcggca
     3121 aaagcgcgtc acggtcggct cgctcacctc ggcgcgctcg gccaggcggt cgatcgcggc
     3181 gtgggtggcg aagtcgggat cggccaggat ttccgcggcg agccgctggt ctgagcggct
     3241 gcccgacgcg gcgatctcgc gcagccgcgc aaccacatcg atggccgggc gctgcagcgt
     3301 ggcgatcaca gccttgcgcc tgtcagttga tcgaagacat ggatgtgagc cgcgtccacg
     3361 ttcagccgca gcacgtcgcc gggcgcagcg gcaatgcgct cgcgaaaaac gcagcgcgcc
     3421 tcggcgccgt cgatggttcc gtagagaagg gtctcggcgc cggtcggctc gatgaccagc
     3481 gccttgaagg gaaagccctc gccttcgccg cataagcggt aatgctccgg ccggatgccg
     3541 atctcgacgc tttgcccgga tgcggcggag gtctcgccaa gcggcaaggc cagccccgat
     3601 ttcgtccgca agagcggcgg gccgtcgccg cgctcgactt ggccgggcac gaaggtcatg
     3661 gcgggcgagc cgagaaagcc ggcgacgaag cggttgcccg gccggtcgta caattcgagc
     3721 ggcgcgccga tctgctcgat gcggccggca tgcatcacca cgatgcgatc ggccatggtc
     3781 atggcctcga tctgatcgtg ggtgacatag atcaccgtcg tcctgaccgc ctgatgcagc
     3841 gccttgatct cggtgcgcat ctgcacgcgc aatttggcgt cgagattgga cagcggctcg
     3901 tcgaacagaa aaacctgcgg atcgcggacg atcgcgcggc ccatcgccac gcgctggcgc
     3961 tggccgccgg agaggtcgcg cgggaagcgg ttcaggaatt gagcgatgcc cagattctcc
     4021 gccactctgg cgatgcgcct gtcgcgctcc tgcctcggca tgcgtgcgat cttcagatgg
     4081 aaaccgaggt tgtccgccac ggtcatgtgg ggatagagag cgtagctctg gaataccatg
     4141 gcgatgtcgc ggtcggccgg cgatcggtcg ttcacgacac gtccgccgat ggcaatctcg
     4201 cccgcggtca attcgtcgag accggcgatc agcttgagca gagtggattt t
//
LOCUS       DATGJW010000539         4549 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_619642, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000539 DATGJW010000000
VERSION     DATGJW010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4549)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4549)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4549
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_619642"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1499
                     /locus_tag="VKU03_16365"
     CDS             <1..1499
                     /locus_tag="VKU03_16365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003609872.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HLJ72890.1"
                     /translation="ITDKAYDGDGRMINSRFLDGMTTSEAFEETARRLSAQTLSGEPQ
                     GKRQVNYKLRDWGISRQRYWGCPIPIVHCPACGAVPVPEKDLPVELPPDVTFDAPGNP
                     LDRHPTWKHVRCPSCGEPAERETDTMDTFVDSSWYFARFTDPRNAAAPTDPAIANRWL
                     PVDQYIGGVEHAILHLLYSRFFTRAMRRCGHLGMDEPFAGLFTQGMVVHETYRSEGGE
                     WIAPADVRIESGPDGRKAFLLEGGAPLEIGPIEKMSKSKRNTVDPDEIIATYGADTAR
                     WFMLSDSPPDRDVIWSEAGVQGAARQAQRLWRLTLEIERIVGASRPAKPAGFSPKALE
                     IRRAAHGALARVEDDIEKLRFNVCIAAIYELGNALAASVGAIEEAPVADDLRFAFAEA
                     GDILIQLFAPMMPHLAEECWEVLGHDKLIAESPWPVCDRALIVTDTITLPVQVNGRKR
                     ADLTIAREADAKAVEAAALALDAVRRAMDNMPAKKVIVVPQRIVNVVV"
     gene            1486..2061
                     /gene="lptE"
                     /locus_tag="VKU03_16370"
     CDS             1486..2061
                     /gene="lptE"
                     /locus_tag="VKU03_16370"
                     /inference="COORDINATES: protein motif:HMM:NF016290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0019867 - outer membrane [Evidence IEA];
                     GO_process: GO:0043165 - Gram-negative-bacterium-type cell
                     outer membrane assembly [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPS assembly lipoprotein LptE"
                     /protein_id="HLJ72891.1"
                     /translation="MSWSDAARLAGAMAIAASLGGCFQPLYGEAAHPGLVEDMRAIQI
                     AEVNPPNPGVKPNAVGLDPQIARQDRVGHYLRNDLIFNFNGTGTTPPPKYRLSVTTTE
                     TTTTPTIESQIGAADAATLTIKAVYVLTPVGGGAPILSSEAVSSAVYDLSMNRFANLR
                     ASRDAEIRLAKSLADEMELRIAAALAEKRGS"
     gene            2218..3492
                     /locus_tag="VKU03_16375"
     CDS             2218..3492
                     /locus_tag="VKU03_16375"
                     /inference="COORDINATES: protein motif:HMM:NF024895.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="outer membrane beta-barrel protein"
                     /protein_id="HLJ72892.1"
                     /translation="MRRYFLLLSLSALAPCSAFAADLPTSKPAPAIFSPAPAADWRGF
                     YAGSFVGATVGVYTTRETGSASFSGSGAGFTTGALAGYNWQTGAFVYGVEGDIGSNSA
                     EHKFAAAPGFVANEVENIYALHGRARLGYDLGAFMPFVAGGLAYGRSEQFQRGALTFD
                     GDTKSRVGWTIGAGVDAKVNLPILGPSILRGEYLYDSYPATSFDLNGPVMRTNIADHT
                     FRVALISRFDEAWRPPAAPDKIDWSGDYFGVLGGGAWDSLTTTGLGASTKFSGSGPIG
                     GVYSGHNWMFGDMVLGIEGATMLADISGHGPQPGAATTNYRDYFQSDFRGRAGYAIGR
                     FLPFFAAGIAYGASRQIDVATGNNQGLVPAWAWTVGLGADYMLTERLAVRAEYLYSRS
                     FSNDQTHLDSVTCCAQRRTDDSLRVGLAYFFH"
     gene            3608..4141
                     /gene="thpR"
                     /locus_tag="VKU03_16380"
     CDS             3608..4141
                     /gene="thpR"
                     /locus_tag="VKU03_16380"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012590937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HLJ72893.1"
                     /translation="MPRLFTGLEIPPDIAADLAMLRGGVAGARWVDVANYHITLRFIG
                     DIDDRTAGEAAAILDEIHRPSFTVTLDGLSWFGADKPRAIVAKVKPTAPLVELQADQE
                     RKLRRVGLEPEPRKYTPHVTLARLRQTSIHAVADYLSSRGYFPSRQFEAKRFVLFSSR
                     ASTGGGPYLVEAAYGLL"
     gene            4336..>4549
                     /locus_tag="VKU03_16385"
     CDS             4336..>4549
                     /locus_tag="VKU03_16385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020012316.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome P450"
                     /protein_id="HLJ72894.1"
                     /translation="MNLSIDAPRRRVALNPRDPAFFNDPYPAYRAIRAAAPVFFWEDY
                     GFWCFARHRDVSALLRDRRFGRQILHV"
BASE COUNT          719 a         1576 c         1469 g          785 t
ORIGIN      
        1 cgatcaccga caaggcctat gacggcgacg gccgcatgat caattcgcgc ttccttgacg
       61 gcatgacgac gagcgaagct ttcgaagaga ccgcgcgccg gctctcggcg caaaccttga
      121 gcggcgagcc gcagggcaag cgccaagtca attacaagct gcgcgactgg ggcatttccc
      181 gccagcgcta ttggggctgc ccgatcccga tcgtccattg cccggcctgc ggcgcggtcc
      241 cggttccgga aaaggacctg ccggtcgaat tgccgcccga cgtgacgttc gatgcgcccg
      301 gcaatccgct cgaccgccat ccaacctgga agcatgtccg ctgcccgtcc tgcggcgagc
      361 ctgcggagcg tgagaccgac acgatggaca cgttcgtcga ttcgtcgtgg tatttcgccc
      421 gtttcaccga tccgcgcaac gccgcggcgc cgaccgatcc ggccatcgcc aatcgctggc
      481 tgcccgtcga ccaatatatc ggcggcgtcg agcacgcgat cctgcatctg ctctattcgc
      541 gcttcttcac ccgggcgatg cggcgctgcg gccatctcgg catggacgag cccttcgcgg
      601 gtctcttcac ccaaggcatg gtggtgcacg agacctatcg ctcggaggga ggcgaatgga
      661 tcgcgccggc cgacgtgcgc atcgaatcgg ggcccgacgg gcgcaaagcc ttcctcttgg
      721 agggcggcgc gccgctcgaa atcgggccga tcgagaagat gtcgaagtcg aagcgcaaca
      781 cggtcgaccc cgacgagatt atcgcgacct acggcgccga cacggcgcgc tggttcatgc
      841 tgtcggattc gccgcccgac cgcgacgtga tctggtcgga ggccggcgtg cagggcgcgg
      901 cgcgccaggc gcagcggctg tggcggctga ccttggagat cgagcggatc gtcggggcga
      961 gccggccggc caagccggcg ggcttctcgc ccaaagcgct ggagatccgg cgcgccgccc
     1021 atggcgccct cgcccgggtc gaggacgata tcgaaaagct gcgcttcaac gtctgcatcg
     1081 cggcgatcta cgagctcggc aatgcgctcg cggcttcggt cggcgcgatc gaggaagcgc
     1141 cggtcgccga cgatctgcgc ttcgccttcg ccgaggccgg cgacattctg attcagctct
     1201 tcgcgccgat gatgccgcat ctcgccgagg agtgctggga ggttctcggt cacgacaagc
     1261 tgatcgccga atcgccctgg ccggtctgcg accgcgcgct catcgtcacc gacacgatca
     1321 ccctgcccgt gcaggtcaac ggccgcaagc gcgccgattt gaccatcgcc cgcgaagcgg
     1381 acgccaaggc ggtggaggcc gcggccctgg ctctcgacgc ggtccggcgc gccatggata
     1441 atatgccggc caagaaggtg atcgtcgtgc cgcaaaggat cgtcaatgtc gtggtctgac
     1501 gccgcccggc tcgccggggc gatggcgatc gcggcctctc tcggcggctg ctttcagccg
     1561 ctttatggcg aggccgcgca tcccggcctg gtcgaggaca tgcgggcgat ccagatcgcg
     1621 gaggtcaatc cgcccaatcc cggcgtcaag ccgaacgcgg tcggcctcga tccgcagatc
     1681 gcccgccagg accgcgtcgg ccattacctg cgcaacgatt tgatcttcaa tttcaacggc
     1741 acggggacaa cgccgccgcc caaatatcgt ttgagcgtga cgacgaccga gacgacgacg
     1801 acgccaacga tcgaatcgca gatcggcgcg gccgacgccg ccaccctgac gatcaaggcc
     1861 gtctatgtcc tgacgccggt cggcggcggc gcgccgattc tctcgagcga ggcggtctcc
     1921 tcggccgtct acgatctgtc gatgaaccgc ttcgccaatt tgcgcgcttc gcgcgatgcg
     1981 gagatccggc tggccaaatc gctggccgac gagatggagc tgcgcatcgc ggcggccctc
     2041 gccgaaaagc gggggagttg acgaagcggc cggaagacgg tctcgttatg aggcagcatc
     2101 gaaacggcgt cattggcgat ttggcgacga gacgtcgtta cgttgaagcg gggcggggct
     2161 aagatcaggg gcaagatgga ttattcgtcc gcttgcccga gtcggggatc agatcgaatg
     2221 cggcgttact tcttgctcct cagcctttcc gccctcgcgc cttgcagcgc cttcgccgcc
     2281 gatcttccga cctccaagcc tgccccggcg attttctcgc cggcgccagc cgcggattgg
     2341 cgcggcttct acgccggctc gttcgtcggc gcgacggtcg gcgtctatac gacccgcgaa
     2401 accggctccg cctcgttttc gggctcgggc gccggtttca ccaccggcgc cttggcgggc
     2461 tacaattggc agaccggcgc ctttgtctat ggcgtcgaag gcgatatcgg ctcgaacagc
     2521 gcagagcata aattcgccgc cgcgcccggt ttcgtcgcca acgaggtcga gaacatctat
     2581 gcgctgcatg gccgggcccg gctgggctac gacctcggcg cgttcatgcc cttcgtcgcc
     2641 ggcggcctcg cctatggccg cagcgagcaa ttccagcgcg gcgccctgac tttcgacggc
     2701 gacacaaaga gccgcgtcgg ctggacgatc ggcgccggcg tcgacgccaa ggtcaacctg
     2761 ccgatcctcg gcccctcaat tttgcgcggg gaatatctct atgacagcta tccggcgacg
     2821 agcttcgatc tgaacgggcc ggtcatgcgc accaacatcg ccgaccatac gtttcgcgtg
     2881 gcgctgatct cgcgcttcga cgaggcttgg cggccgcccg ccgccccgga caagatcgac
     2941 tggtcgggcg actatttcgg cgtgctcggc ggcggcgcct gggattcgct gaccacgacc
     3001 gggctcggcg cgagcacgaa attcagcggc agcggcccga tcggcggcgt ctattcgggc
     3061 cacaactgga tgttcggcga tatggtgctc gggatcgagg gcgcgaccat gctcgccgac
     3121 atttccggcc acgggccgca gcccggcgct gcgacgacca attatcgcga ctatttccag
     3181 agcgatttcc gcggccgcgc cggctatgcg atcggccgat tcctgccctt cttcgccgcc
     3241 gggatcgcct atggggcgag ccggcagatc gatgtcgcga ccggcaataa tcaaggcctc
     3301 gtcccggctt gggcttggac ggtcggcctc ggcgccgatt acatgctgac cgagcgcctc
     3361 gcggtgcgcg ctgaatatct ttattcgcgc tccttctcga acgatcagac ccatctcgac
     3421 agcgtgacct gctgcgcgca gcggcgcacc gacgacagcc tcagggtcgg cctcgcctat
     3481 ttcttccact gaccgaaagg ctccgagcgg cggcgcggcg gcgggccgtc gcgccagagt
     3541 cgcgaatcgc ggctctcgat cgctccgaaa aacattggcc tccggccgtc aaaaggcgtt
     3601 cttagaaatg ccccgcctgt ttaccggcct cgaaatccct cccgacatcg ccgcggatct
     3661 cgcgatgctg cgcggcggcg tcgccggcgc gcgctgggtg gatgtcgcga attaccacat
     3721 caccttgcgc ttcatcggcg atatcgacga tcgcaccgcc ggcgaggccg ctgcgatcct
     3781 cgacgagatt catcgcccgt ccttcaccgt gacgctggac ggcctgagct ggttcggcgc
     3841 cgataagccg cgcgccatcg tcgcgaaggt gaagccgacc gcgccgctcg tcgaattgca
     3901 ggccgaccag gagcgcaagc tgcgccgcgt cgggcttgag ccggagccgc ggaaatatac
     3961 cccgcatgtg acgctggcgc gtctgcgcca gacgtcgatc cacgccgtcg ccgactatct
     4021 cagctcgcgc ggctatttcc cctcgcggca attcgaggcg aaacggttcg ttctgttctc
     4081 ctcgcgcgcc tcgaccggcg gcggcccgta cctggtcgag gcggcctatg ggctattgta
     4141 ggggatcatt cccttctccc cgcgcagcgg ggagaagtca gcccgaaggg ccggatgagg
     4201 ggctgcgcag gactggaagg gtacgcgccc tgcaaacgct aaccggcaag ctcggcgcag
     4261 cccctcaccc ggcggcttcg ccgccaccct ctccccgctg cgcggggcga ggggactttc
     4321 gcgcatatcg ctgacatgaa cctctcgatc gacgctcccc gccgccgcgt cgccctcaac
     4381 ccgcgcgatc cggccttctt caatgacccc tatcccgcct accgcgcgat ccgcgcggcc
     4441 gcgccggtct ttttctggga ggattacggc ttctggtgct tcgcgcgtca tcgggacgtc
     4501 agcgccctgc tgcgcgaccg ccgcttcggc cggcagattc tgcatgtcg
//
LOCUS       DATGJW010000540         4307 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1035152, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000540 DATGJW010000000
VERSION     DATGJW010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4307)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4307)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4307
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1035152"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1416)
                     /locus_tag="VKU03_16390"
     CDS             complement(<1..1416)
                     /locus_tag="VKU03_16390"
                     /EC_number="3.5.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008973547.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidase"
                     /protein_id="HLJ72895.1"
                     /translation="MTPHQLSAVELLAAYRDGKLSPLEAMKDVLARVERFEPHIKATY
                     LLNPERALADARASEARWRRGEPIGPLDGVPATVKDNIPTKGEPTPLGTAASELIPAP
                     ADAPPAARLREAGAIIFSKTTMPDYGMLSSGLSSFHPLARNPWDLSRNPGGSSAGAGA
                     AAAAGYGPLHLGTDIGGSVRLPAGWCGVFALKPSLGRVPIDPPYLGRVAGPMTRRVED
                     SALMMSVLSKPDPRDYMNLPPQELDWRIKPAALRGLKIGLLLDVGCGAPPSPETRAAI
                     EKAARDLALAGAVVEPLPPFLTQEMLDGWDHFFRMRSYLDIAALPIERRVKILPYILA
                     WAESAAPLSGQDVGRGYSQIMAMRRATVAATLPYDFVLSPTAPMVAFAAEDPSPGNDP
                     LDPFTHIGFTLPFSVSEQPAASINCGYSPEGLPIGLQIIGRRFDDLGVLSFARAFEEM
                     RRGEARPWPEPPTYFSSPAKAG"
     gene            complement(1597..3081)
                     /locus_tag="VKU03_16395"
     CDS             complement(1597..3081)
                     /locus_tag="VKU03_16395"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012046322.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HLJ72896.1"
                     /translation="MRKSFRLIIAAGAALFASAAFAAPKDSVTIGMVLEPPGLDPTTG
                     AAAAIGEITHYNIYEGLTKINADFSVTPLLADSWTLSPDLKTFTFKLKSGVKFQDGEP
                     FSSKDVKFSFERAASKDSTNKDKAFFASIDKIDTPDPQTVALTFKQPSFEALFHLGMN
                     TAVIVDEKSEATNATNPIGTGPYKMTNWNKGSSVTLEKWAGFRDPSKIAIAKATFRII
                     NDPAAQVAALLSGDVDDFPRFGGVQSVDQFKNDPRFQVIIGGTEGKTILAMNNKRKPL
                     DELKVRQAIAYAIDRKAIIDGAMNGYGVPIGSHLTPNDPGYIDLTGEYPHDPAKAKAL
                     LKEAGVTTPLSLSLILPPPAYAREGGQIIAAELAEVGIQAKIENVEWAQWLSGVYKGH
                     NYDLTIISHVEPLDIDIYANPDYYFGYDNKDFQAIIAKMNAAPDLATFKKYIGEAQKK
                     IADDCVNAFLFQLQQITIANKNLQGLWKDSPIFANDLSAMSWKQ"
     gene            complement(3428..3937)
                     /locus_tag="VKU03_16400"
     CDS             complement(3428..3937)
                     /locus_tag="VKU03_16400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012067439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2852 domain-containing protein"
                     /protein_id="HLJ72897.1"
                     /translation="MSTAYGAAAGGSGPSQEWASNDWRGGAWRSHRCGFARWTPIELL
                     AMILGFIVFWPIGLAILGFKVWQRKSGYPGDLGTAAQEKWREARSAFSAGPWGGRAPW
                     RATGNMAFDEWRAAEIQRLEEERRKLDEAQKEFAAFVENIRRAKDREEFERFMNERRK
                     GPEQPGPSA"
BASE COUNT          736 a         1345 c         1456 g          770 t
ORIGIN      
        1 ccccgctttc gcgggggagg aaaagtacgt cggcggctcg ggccagggcc gcgcttcgcc
       61 gcgccgcatc tcctcgaagg cccgggcgaa gctcagcacg ccgagatcgt cgaatcgcct
      121 gccgatgatc tgtagcccga tcggcaggcc ttccggcgaa tagccgcaat tgatcgaggc
      181 ggccggctgc tcggagacgc tgaagggcag ggtgaagccg atatgggtga aggggtcgag
      241 cggatcgttg cccggcgacg ggtcctcggc ggcgaaggcg accatcggcg cggtcggcga
      301 cagcacaaaa tcatagggaa gcgtcgccgc gaccgtcgcg cggcgcatcg ccatgatctg
      361 gctgtagccg cggccgacat cctggccgga tagcggcgcc gccgattccg cccaggcgag
      421 gatgtaggga aggattttca cgcggcgctc gatcggcaag gccgcgatgt cgagatagga
      481 gcgcatgcgg aagaaatggt cccagccgtc gagcatttcc tgggtgagga aaggcggcag
      541 gggctcgacg accgcgccgg caagggcgag atcgcgcgcc gccttctcga ttgccgcgcg
      601 cgtctccggc gaaggcggcg cgccgcagcc gacatcgagc agcagcccga ttttcaggcc
      661 gcgcagcgcg gccggcttga tccgccaatc caattcctgc ggcggcaggt tcatgtagtc
      721 gcgcggatcg ggcttcgaca gaacgctcat catcagcgcg gaatcctcga cgcgccgcgt
      781 catcggtccg gccacccggc cgagataggg cggatcgatc ggcacgcggc cgaggctggg
      841 cttgagcgcg aaaacgccgc accagccggc cggcaggcgg accgagccgc cgatatcggt
      901 gccgagatga agcgggccgt agccggccgc cgccgcggcg ccggcgccgg ccgacgagcc
      961 gccgggattg cggctcaagt cccatggatt gcgagcgagc gggtgaaagc tcgataggcc
     1021 ggacgacagc atgccgtaat ccggcatggt ggtcttcgag aagatgatcg cgccggcttc
     1081 gcgcaagcgc gccgcaggcg gcgcgtcggc gggcgccggg atcagttcgc tcgccgccgt
     1141 gccgagcggc gtcggctcgc ccttggtcgg aatgttgtcc ttcaccgtcg ccggaacgcc
     1201 gtcgagcggc ccgatcggct cgcctcgccg ccagcgcgct tccgaagccc ttgcgtcggc
     1261 gagcgcgcgc tcgggattga gcaggtaggt cgccttgata tgcggctcga agcgttcgac
     1321 gcgggcgagc acgtccttca tcgcttcgag cggcgagagc ttgccgtcgc gataggcggc
     1381 gagcagttcg acggcggaga gttgatgtgg ggtcatttcg agaatggctt cgccgcgcgt
     1441 tgcgtcacga gcgggcggcg ttggtgtgat gacgaatcga aagaagctgc gcccgtcact
     1501 gcgagcgaag cgaagcaaat ccagtcatag ggcggcgctc tacgagtgga ttgctttgtc
     1561 gcttcgcttc tcgcaatgac gattgcgcaa gtcgttctac tgcttccagg acattgccga
     1621 caggtcgttg gcgaagatcg gcgaatcctt ccacagccct tgcaggttct tgttggcgat
     1681 ggtgatctgc tggagctgga acaggaacgc gttgacgcaa tcgtcggcga tcttcttctg
     1741 cgcctcgccg atatacttct tgaacgtcgc gaggtcgggg gcggcgttca tcttcgcgat
     1801 gatcgcctgg aagtccttgt tgtcgtagcc gaaataatag tcgggattgg cgtagatatc
     1861 gatgtcgagc ggctcgacat ggctgatgat ggtcaggtcg taattgtggc ccttatagac
     1921 gcccgacagc cattgcgccc attcgacgtt ctcgatcttg gcctggatgc cgacttcggc
     1981 gagctcggcg gcgatgatct gaccaccctc gcgggcatag gccggcggcg gcaggatcag
     2041 cgacaggctg agcggcgtcg tcacgcccgc ttctttgagc agcgccttcg ccttcgccgg
     2101 atcgtgcgga tattcgccgg tcaggtcgat atagccggga tcgttcggcg tcaggtgcga
     2161 gccgatcggc acgccgtagc cgttcatcgc gccgtcgatg atcgccttgc ggtcgatcgc
     2221 ataggcgatc gcttggcgta ctttgagctc gtcgagcggc tttctcttat tgttcatcgc
     2281 gaggatcgtc ttgccctcgg tcccgccgat gatcacctgg aagcgcggat cgttcttgaa
     2341 ctggtcgacg ctctgcacgc cgccaaagcg cgggaaatcg tcgacgtcgc cggacagcaa
     2401 ggcggcgact tgcgcggccg ggtcgttgat gatgcggaac gtcgccttgg cgatggcgat
     2461 cttgctcgga tcgcggaagc cggcccattt ctccaaagtg acggacgagc ccttgttcca
     2521 attggtcatc ttatacgggc cggtgccgat cggattggtt gcgttggtcg cttcgctctt
     2581 ctcgtcgaca atcacggcgg tgttcatgcc gagatggaac agagcctcaa agctcggctg
     2641 cttgaaggtg agcgcgaccg tctgagggtc gggcgtgtcg atcttgtcga tcgaggcgaa
     2701 gaacgcctta tccttgttgg tcgagtcctt cgacgccgcg cgctcgaagg agaatttcac
     2761 gtccttcgac gagaagggct cgccatcttg gaacttgacg ccggacttga gcttgaacgt
     2821 gaaggtcttc agatcgggcg acaaggtcca gctatcggcg agcaaaggcg tgaccgagaa
     2881 gtcggcgttg atcttcgtca agccctcata aatgttgtag tgcgtgattt cgccgatcgc
     2941 cgccgcagcg ccggtcgtcg gatcgaggcc gggcggctcc agcaccatgc cgatggtgac
     3001 gctgtccttc ggcgcggcga aagccgccga ggcgaataat gcggcgcccg ccgcgatgat
     3061 gagacgaaac gacttacgca tcggatctcc taaagccccg catcattctt caaaaaagct
     3121 gagcgagcgg cttgaggccg aatcatcgtc attgcgagga gcgaagcgac gaagcaatcc
     3181 agaagcctcg gcggttcctg gattgcttcg cttcgctcgc aatgacggtt gaggtcccac
     3241 gccaagccat cgcgcgccgt ctatagcaat cccagggcgc gctcgccgtc aagcgccacg
     3301 ccgcggtcgc tcgccgcggc cagagcgcaa ggcgcatgac tttggccgcg gtcgacgacc
     3361 gcggctacaa aaaaaccgcg gcgccggagg acggcgccgc ggttgcggca acgatagaac
     3421 ggatcgctca ggcgctcggg cccggctgct ccggcccttt gcggcgctcg ttcatgaacc
     3481 gctcgaactc ttcgcggtct ttcgcccggc ggatgttttc gacaaaggcg gcgaattcct
     3541 tctgcgcctc gtcaagcttg cgacgctcct cttcgagtct ttgaatctcg gcggcgcgcc
     3601 attcgtcgaa ggccatgttg cccgtggcgc gccacggggc gcggccgccc caaggacccg
     3661 cggagaaggc gctgcgcgcc tcgcgccatt tttcctgcgc cgccgttccg agatcgcccg
     3721 gatagcctga tttgcgctgc cagaccttga agcccagaat cgcgagcccg atcggccaga
     3781 agacgatgaa tccgaggatc atcgccagga gctcgatcgg cgtccagcgc gcgaagccgc
     3841 agcgatggct gcgccaagcg ccgccgcgcc agtcgttcga cgcccattcc tgggaaggac
     3901 cagaaccgcc ggcggcggcg ccgtaagccg tgctcatcaa gagcctccca taagacgccg
     3961 cttccgcctt cgcgcgccac gcgcggagcg aacgacggca aaccaacgct gagacagatt
     4021 tggtccaaat ggggcgcccg caagagggcg tcgcgaatat ttttcagagc cgcctgatcc
     4081 tcccccgtga agcgaagcga aacgggggag ggggaccgcg cgtcagcgcg gtggaggggg
     4141 cctccggcgg ttggggttat cctgctagac cggcgccccc tccaccatgc ttcgcatggt
     4201 ccccctcccc cgtcttcgac gggggaggat ggtcccccgc tttctcgggg gaggatgatc
     4261 aatccaaagg gaaatggcac gccgcctggc gcccgtcggc gagcggc
//
LOCUS       DATGJW010000541         5930 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_933089, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000541 DATGJW010000000
VERSION     DATGJW010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5930)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5930)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5930
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_933089"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1180
                     /gene="betC"
                     /locus_tag="VKU03_16405"
     CDS             <1..1180
                     /gene="betC"
                     /locus_tag="VKU03_16405"
                     /EC_number="3.1.6.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR03417.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="choline-sulfatase"
                     /protein_id="HLJ72898.1"
                     /translation="PADYGWTPDWDMPLERPDWYHNMSSVTDAGLCVRTNQLDFDDEV
                     TFAAERALYDIARSNDKRPFLLVASLTHPHDPFAITERYWNLYRDDEIDMPGPALPKD
                     KLDPHSLRLRHVCAMDAQEITQDQIRNARRAYYGAISYVDENLGRLMHALRAAGLADD
                     TIVLFLGDHGEMLGERGLWFKMTFFEGGARVPLIVSAPQRFAPRRVKGSVSLVDILPT
                     LVDLAGGDVAALSGATDGRSLRPHLESGAGHDEVIGEYLAEGAVAPIVMIRRDKWKFI
                     HSPADPDQLYDLAADPKERDNLAARPEHTGRVAEFRAEIARRWDLAALDAEVRESQRR
                     RRVVNAALTKGETRAWDFQPFKDAGKSYVRNTIPLDDLEAMARFPRVTSPLPKGEEQG
                     "
     gene            1302..3338
                     /locus_tag="VKU03_16410"
     CDS             1302..3338
                     /locus_tag="VKU03_16410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011747520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADH:flavin oxidoreductase"
                     /protein_id="HLJ72899.1"
                     /translation="MSTDPLLQPYRLKHLTLKNRIMITAHEPSYAEDGMPKERYRAYH
                     AERAKGGVALTMTAGSAAVSRDSPPAFNNILAYKDEVVPWIRDLADACHEHGTAVMIQ
                     LTHLGRRTRWDYGDWLPALSPSHKREPAHRAFPKKLEDWDIERIVSDYADAAERMKAG
                     GLDGIELEAYGHLMDQFWSPLTNTLDGPYGGALDNRLRFTFDVLSAIRRRVGPDFIVG
                     LRYSADEVAEGGLTKKDGLEISRRLAASGMVDFLNIIRGHIDTDAALTDVIPIQGMAS
                     APHLDFAGEIRAATGFPTFHAARIADVATARYAIASGKLDMVGMTRAHMTEPHIVRKI
                     MERREDDIRPCVGANYCLDRIYQGGAAFCIHNPSTGREETVPHEIVKAPAARKIVIVG
                     AGPAGLEAARVAAERGHEVVVFETASAPGGQVRLTARSQRRREMMGIIDWRMAQCEKN
                     GVAFRFNLWAEAEDALAQNPDVAIVATGGFPQTEILSAGDELVVSAWDIISGDVKPGA
                     NVLLFDDAGDHAALQAAEFIAESGAKLEVMTPDRAFAPEVMGMNLVPYMRSLQKRDVT
                     FTVTFRLEAVRRDGNELIAQIGSDYGGVRQERRVDQVIVNHGTLPLDDLYFALKPLSR
                     NLGAVDYAALLAGRGQNMVRNAAGKFELFRIGDAVSARNTHAAIYDALRLVRTL"
     gene            complement(3437..4129)
                     /locus_tag="VKU03_16415"
     CDS             complement(3437..4129)
                     /locus_tag="VKU03_16415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012651714.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72900.1"
                     /translation="MVSNNSFAQVGLAIRSAGLVPRGALRLDEAERHGPLAECATIVL
                     IGVAGRQGWSAFAGSPERLDGAPHPLDRWSQRVIGALAKALGCRALYPFGGPPYWPFP
                     RWAMRAEPLHVSPLGMLIHPDYGLWHSYRGALAFSEALQIPALESRPSPCESCAARPC
                     LKACPVGAFSSDGYDAARCAAHLRSGTGRDCMEGGCLARRACPIGVEFAQSEEQARFH
                     MKAFLAAREGGG"
     gene            complement(4153..>5930)
                     /locus_tag="VKU03_16420"
     CDS             complement(4153..>5930)
                     /locus_tag="VKU03_16420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006315386.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HLJ72901.1"
                     /translation="EMGVLRESDSSWYMREEAGGLLLGPYEVGAPACYVDGPSPGSEY
                     ELFQEDLERLTPHIETAIARVPAFGEVGIKKVYNGAIAYTPDGSPIVGPAPGLRNFWL
                     NEGHSFGVTAAGGAGWQLAEWITEGEPTIDMLGVDPRRFGPYAQRGFLVEKNEEAYAK
                     VFTNHYPDEERAAARPLRQAPCYGRMRDLGAVFGSVYGWERPNWFAPPDYALSEKDLN
                     KPDVLLNENHPPAGAGGRVREKWSFRRSNYFDFVGAECRNVHENVGLQDMSPFAKFEV
                     SGPGAEGWLNAILTNRIPKALHRTALTYLLTARGGVRAEFTLTRLGPQRFYLVSAGAL
                     EAHDYDCLQKLLPSDGSVRLDKVTTQYGVLVLAGPRSREVLAKVADIDVSDKAFPWLT
                     ARPLSIGAAGLLALRVNFVGELGYEFHHPIEMQNYIFDRLMAAGASFGIKPYGIRAMD
                     SLRLEKSYKLVGRELSIEYAAFESGLDRFVDLKKGDYLGREALLAWRAKGFANRLVTM
                     EVLDPRDADARGSEPVFRNGASVGRTTSGGYGWRTGKSLALAMVRPDCAEIGTELDIQ
                     VLGEKRRAVVIHDSPFDPENARLRA"
BASE COUNT         1064 a         2028 c         1885 g          953 t
ORIGIN      
        1 cccggccgat tacggttgga cgccggattg ggacatgccg ctcgaacgac ccgattggta
       61 ccacaatatg agctcggtca ctgacgccgg tctctgcgtg cgaaccaatc agctcgattt
      121 cgacgacgag gtgaccttcg cggcggaacg cgccctctat gatatcgccc gctcgaacga
      181 caagcgcccc tttttgcttg tcgcctcgct cacccacccg cacgatcctt tcgcgatcac
      241 cgagcgctat tggaatctct accgcgacga cgagatcgat atgcccggcc cggccttgcc
      301 gaaagacaag ctcgatccgc attcgctgcg cttgcgccat gtctgcgcca tggacgcgca
      361 agaaatcacg caggaccaaa tccgcaacgc ccgccgcgcc tattacggcg cgatttctta
      421 tgtcgatgaa aatctcggcc ggctcatgca cgccctacgc gcggccggcc tggccgacga
      481 cacgatcgtc ctttttttgg gcgatcacgg tgaaatgctt ggcgagcgcg gcctctggtt
      541 caagatgacc ttcttcgagg gcggcgcgcg cgtgccgctg atcgtctcgg cgccgcagcg
      601 cttcgcgccg cggcgggtca agggcagcgt ttcgctggtc gatatcctgc cgactttggt
      661 cgacctggcg ggcggcgacg tcgccgcgct gtcgggcgcg accgacggcc gcagtttgcg
      721 gccgcatctc gaaagcggcg ccggccatga cgaggtcatc ggcgaatatc tcgccgaagg
      781 cgcggtggcg ccgatcgtga tgatccgccg cgacaagtgg aaattcatcc actcgccggc
      841 cgatccggac cagctctacg atttggccgc cgatcccaag gagcgcgaca atctcgccgc
      901 gcggccggaa cataccggcc gggtcgcgga gttcagggcg gagatcgcgc ggcgctggga
      961 ccttgcggcg ctcgacgccg aggtgcgcga gagccagcgt cgccgccgcg tcgtcaacgc
     1021 cgccctgacc aagggcgaaa cgcgcgcctg ggatttccag ccgttcaagg acgccggaaa
     1081 atcgtacgtg cgcaacacca tcccgctgga tgatctcgaa gccatggcgc ggtttccccg
     1141 tgtaacctcc cctctcccga agggagaaga gcaggggtga ggggtacggc ggtcgtggag
     1201 aaaagcgcga tggtcgaata agcacattat caaaatattc ctggcggcga tcccctcacc
     1261 cctgcccctc tcctccgcga gagaaggaaa ggctccgctc catgtccacc gaccctctcc
     1321 ttcagccgta ccgcctcaag catctgacgc tgaagaaccg gatcatgatc accgcgcatg
     1381 agccgtccta cgccgaggac ggcatgccga aagagcgcta ccgcgcctat catgccgagc
     1441 gggcgaaggg cggggtcgcg ctgaccatga cggcgggctc ggccgcggtg tcgcgcgaca
     1501 gcccgccggc cttcaacaac atcctcgctt acaaggacga ggtggtgccg tggatcaggg
     1561 atttggccga cgcatgccac gagcacggga cggcggtgat gatccagctc acccatctcg
     1621 gccgccgcac ccgttgggat tacggcgact ggctgcccgc gctctcgccc tctcacaagc
     1681 gcgagccggc ccatcgcgcc tttccgaaaa agctggagga ttgggacatc gagcggatcg
     1741 tcagcgacta tgccgacgcc gccgagcgga tgaaggcggg cgggctcgac gggatcgagc
     1801 tcgaagccta tggccacctc atggaccagt tctggtcgcc gctgaccaac acgctggacg
     1861 gaccttacgg cggcgcgctc gacaatcggc tgcgctttac cttcgacgta ctctccgcga
     1921 tccgccgccg cgtcggcccc gatttcatcg tcggcctgcg ctacagcgcc gatgaagtcg
     1981 cggaaggcgg gctgacaaaa aaggacggtt tggagatttc gcggcgcctg gcggcgagcg
     2041 gcatggtcga cttcctcaac atcattcgcg gccatatcga caccgacgcg gctttgaccg
     2101 atgtcattcc gatccagggc atggcgagcg cgccgcatct cgattttgcc ggcgaaatcc
     2161 gcgccgcgac gggattcccg accttccacg ccgccaggat cgccgatgtc gccaccgcgc
     2221 gatacgccat cgcctcgggc aagctcgata tggtcggcat gacgcgggcg catatgaccg
     2281 agcctcatat cgtccggaaa atcatggagc gacgcgagga cgacatccgc ccttgcgtcg
     2341 gcgcgaatta ctgcctcgac cgcatctacc aaggcggcgc ggcgttttgc atccacaacc
     2401 cgtccaccgg ccgcgaggag acggtcccgc atgaaatcgt caaggcgccg gccgcgcgca
     2461 aaatcgtgat cgtcggcgcc ggaccggccg ggctcgaagc ggcgcgggtg gcggccgagc
     2521 gcggccatga ggtcgtcgtc ttcgagacgg cgagcgcgcc cggcggccag gtgcgcctca
     2581 ccgcgcgcag ccaacgccgc cgcgagatga tggggatcat cgattggcgc atggcgcaat
     2641 gcgaaaaaaa cggcgtcgcc ttccgtttca atctctgggc cgaggccgag gacgccctgg
     2701 cgcaaaatcc cgatgtcgcg atcgtcgcca ccggcgggtt tccgcaaacc gagatcttaa
     2761 gcgccggcga cgagcttgtc gtctcggcct gggacatcat ttccggcgac gtcaagccgg
     2821 gcgccaacgt gcttctgttc gacgacgccg gcgatcacgc ggcgttgcag gcggccgagt
     2881 tcatcgccga atccggcgcc aaattggagg tgatgacgcc ggaccgcgcc ttcgcgccgg
     2941 aagtcatggg catgaatctc gttccctata tgcgcagcct gcagaagcgc gatgtgacct
     3001 tcaccgtgac tttccggttg gaggccgtgc gccgcgacgg caacgaattg atcgcgcaga
     3061 tcggcagcga ctatggcggc gtgcgccagg agcggcgcgt cgaccaggtg atcgtcaacc
     3121 acggcacgtt gccgctggac gacctctatt tcgcgctgaa gccgctgtcg cgcaatctcg
     3181 gcgcggtcga ctatgcggcg ctccttgccg gccgcggcca gaatatggtg cgaaacgccg
     3241 ccggcaaatt cgaactgttc cggatcggcg acgccgtgtc agcgcgcaac acccacgcgg
     3301 cgatctacga cgcgctgaga ctggtgcgca ctctctgaaa ccgccatcgc gcgcgaagcg
     3361 cgccaatcct cccctcgagc gcgccctgtg actgcattgc ttcgtcgctt cgctccttga
     3421 aaaggacggg acaacgctac ccgccgcctt cgcgagcggc caagaatgct ttcatatgga
     3481 agcgcgcctg ctcttcgctt tgcgcaaatt cgacgccgat cggacacgcc cgccgggcga
     3541 gacagccgcc ttccatgcaa tcgcgccccg tcccgcttcg caggtgcgcg gcgcaacgcg
     3601 ccgcgtcgta gccgtccgat gagaaagcgc cgaccgggca agccttcagg cagggccgcg
     3661 cggcgcagct ttcgcacgga cttggccgcg attcgagcgc cggaatttgc agcgcttccg
     3721 aaaaagccag cgcgccgcga taggaatgcc agagcccgta atccggatgg atcaacatgc
     3781 cgagcggcga gacatgcagc ggctcggcgc gcatcgccca gcgcggaaaa ggccaataag
     3841 gcggcccgcc gaaagggtac aaggcgcgac agcccaaagc cttcgcgagc gccccgatca
     3901 cgcgctgcga ccagcggtcg agcggatgcg gcgcgccgtc aaggcgctcg ggcgatccgg
     3961 cgaaggcgct ccagccctgg cggccggcga cgccgatcag cacgatcgtc gcgcattcgg
     4021 ccagcgggcc atgccgctcg gcttcgtcga gccgcaacgc gccgcgcgga acgagaccgg
     4081 cgctgcggat tgcaagaccg acctgagcaa aagagttgtt cgagaccatt gtcttgcggg
     4141 aacgacaggc cgctatgccc gcaggcgcgc attctccgga tcgaacgggc tgtcatggat
     4201 caccaccgcc cggcgctttt cgccgagaac ctgaatgtcg agctcggtcc cgatctcggc
     4261 acagtccggc cgcaccatcg ccaaggcgag cgacttgccg gtccgccagc catagccgcc
     4321 cgaggtcgtg cgcccgaccg acgcgccgtt cctgaagacc ggctccgatc cgcgcgcatc
     4381 cgcatcgcgc gggtccaaca cctccatcgt gaccaggcga ttggcgaagc ccttggcgcg
     4441 ccaggcgagc agcgcctccc ggccgaggta atcgcctttc ttgaggtcga cgaaacggtc
     4501 gagcccggac tcgaacgccg catattcgat cgacaattcg cggccgacca gcttgtacga
     4561 tttctccaag cgcaacgaat ccatcgcccg gatgccgtag ggcttgatgc cgaacgacgc
     4621 gccggcggcc atcaggcgat caaaaatgta attctgcatc tcgatcgggt ggtggaattc
     4681 atagccgagc tcgccgacga aattgacccg cagcgccaga agcccggccg cgccgatcga
     4741 cagcggccgc gccgtcagcc aggggaaggc cttgtccgaa acgtcgatat cggcgacctt
     4801 ggcgaggact tcgcgcgagc gcggcccggc caggaccagg acgccatatt gcgtcgtcac
     4861 cttgtcgagc cgcacgcttc cgtcgctcgg caagagcttc tgcaggcaat cgtaatcatg
     4921 cgcttcgagc gcgcccgctg aaaccaggta gaagcgctgc ggcccgagcc gcgtcagcgt
     4981 gaattcggcg cgcacgccgc cgcgcgcggt gagcagatag gtcagcgccg tgcgatggag
     5041 cgccttgggg atgcgattgg tgagaatggc gttgagccag ccttccgcgc ccgggccgct
     5101 gacctcgaac ttggcgaagg gcgacatgtc ctgcaggccg acattctcat gcacgttgcg
     5161 gcattcggcc ccgacaaagt cgaaatagtt cgagcggcgg aagctccatt tctcgcgcac
     5221 ccgcccgccc gcgccggccg gcgggtgatt ttcgttgagc agaacgtcgg gcttgttcaa
     5281 gtccttttcg ctcagcgcgt agtccggcgg cgcgaaccag ttcggacgct cccagccata
     5341 gaccgagccg aacacggcgc cgagatcgcg catccggccg tagcacggcg cctggcgcag
     5401 cggccgcgcc gcggcgcgct cctcgtcggg gtaatggttt gtgaagacct tggcgtaggc
     5461 ctcctcgttc ttctcgacaa gaaagccccg ttgcgcgtaa gggccgaacc gccgcggatc
     5521 gacgccgagc atgtcgatcg tcggctcgcc ctccgtgatc cattccgcca attgccagcc
     5581 agcgccgccg gccgcggtga cgccgaagga atggccctcg ttgagccaga aattcctcaa
     5641 gcccggcgcc gggccaacga tcggcgagcc gtccggcgta taggcgatgg cgccgttata
     5701 gactttcttg atgccgacct cgccgaaagc cgggacgcgc gcgatcgccg tctcgatatg
     5761 cggcgtcagg cgctccaaat cctcctggaa cagttcgtat tcgctgcccg gcgacggccc
     5821 gtcgacatag caggccgggg cgccgacctc ataagggccg agcagcaggc cgcccgcctc
     5881 ctcgcgcatg taccaggaac tgtcggattc gcgcagcacg cccatttccg
//
LOCUS       DATGJW010000542         6251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_725402, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000542 DATGJW010000000
VERSION     DATGJW010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 6251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6251
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_725402"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(569..1129)
                     /gene="cobU"
                     /locus_tag="VKU03_16425"
     CDS             complement(569..1129)
                     /gene="cobU"
                     /locus_tag="VKU03_16425"
                     /EC_number="2.7.1.156"
                     /EC_number="2.7.7.62"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018698247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0043752 - adenosylcobinamide kinase
                     activity [Evidence IEA];
                     GO_process: GO:0009236 - cobalamin biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional adenosylcobinamide
                     kinase/adenosylcobinamide-phosphate guanylyltransferase"
                     /protein_id="HLJ72902.1"
                     /translation="MQTASAPLACRSVLILGGARSGKSRYALDLVEKAAPSRLFIATA
                     SPGDAEMAARIKRHQAERGEGWTTLEVERDLVAALGAAREGRAVLVDCLTLWLANLAF
                     EKSDIAAEIERLCDAAGALQGPVVFVSNEVGLGIVPETPLGRDFRDWQGRLNQSMAAS
                     CDAVLFIAAGLPISLKPAPAPSLALR"
     gene            1225..2373
                     /locus_tag="VKU03_16430"
     CDS             1225..2373
                     /locus_tag="VKU03_16430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003604733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase"
                     /protein_id="HLJ72903.1"
                     /translation="MNPIFANVETSVFETMSRLARERQAVNLGQGFPDDPGPADVRAK
                     AAEAVIDGWNQYPPMMGLAELREAVAAHYKRFQDLDLDPSAEIMVTSGATEALAGALL
                     ALIEPGDEVVLFQPMYDAYLPLVRRAGGVPRFVTLRPPDWRIRESDLAKAFSDRTRVV
                     LFNNPLNPAGVVYDREALALLARFCVRRDAIAVCDEVWEHVLFDGRAHIPLIAMDGMR
                     ERTVKIGSAGKIFSLTGWKVGFVCAAPALMRALAKAHQFITFTTPPNLQSAVAYGLGK
                     EDGYFLSMRAALQRSRDRFSRGLTERGFTVLPSAGTYFLNIDIAPLGRSDDLAFCTEL
                     VKEQGVAAIPVSAFYAEDAVKTVVRFCFAKRDSTLDDALARLDAIRPR"
     gene            2383..3537
                     /locus_tag="VKU03_16435"
     CDS             2383..3537
                     /locus_tag="VKU03_16435"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HLJ72904.1"
                     /translation="MKLALATIALAFCASFAVAAERSPFAPPAKPQPHFIHLLAFGDY
                     FDANALAEFESRSGRQIAYDAYGAVSEIAAKLRDGHYDLVVLPGPALREEIAAGALQK
                     IDEARLANAGAVAPRVAAKLEAYDPSGAYALPYMWLVTGLLLDESKIQARLGAGPVSW
                     GLIFSPDLARRFADCGVATPNDRDNMFMAAWRYMGVNPARLNALEIKRAAEILIRLKM
                     GARAFSSPDIDGALANGSACVSVGAEIDARRAMERAKQSGQPISVRFVIPREGAPMSI
                     DSFAIPKDAPHPDDAYALLDFLLRPDIAARNARATGVASGDDGGDEEAFKRLSPEGAI
                     SPALEALVDQEWTRIKGGEKEGASGKKANPHILTIEPRGGGKAKRAPRPR"
     gene            3728..4141
                     /locus_tag="VKU03_16440"
     CDS             3728..4141
                     /locus_tag="VKU03_16440"
                     /inference="COORDINATES: protein motif:HMM:NF016701.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GFA family protein"
                     /protein_id="HLJ72905.1"
                     /translation="MTITGSCLCKQIRYSIAAAPLITRACWCRVCQYFAAGNAAISAC
                     FRRDAVTIEGETKDYVSIADSGAVMHRRFCPTCGVHLFSEAESRPHLIFVRVGTLNDP
                     ELVRPMANIWTASAPSWACMDEGLPRFEGQPPPAG"
     gene            4212..5993
                     /locus_tag="VKU03_16445"
     CDS             4212..5993
                     /locus_tag="VKU03_16445"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018238230.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HLJ72906.1"
                     /translation="MAGTRKLVAILAADVVGFSRLASKDEDRTLARLRALRSDLIDPT
                     VAIHNGRVFKRTGDGALVEFRSVVDAVRCAIEVQNAMVERNAGVPPERRIEFRVGIHL
                     GDVVEEADGDLMGDGVNIAARLEGVAKPGAICLSEDAYRQVKARLDISVSDLGATPLK
                     NIAEPVHAYLLQVGLPAQAKGGAPALAEKPAGPPAPLDKPSIAVLAFQNMSGDPEQEY
                     FADGIAEDVLTDLSKIGGLLVIARNSSFAYKGKPVDIRAIARELGVRSVLEGSVRRAG
                     NRVRITAQLIDAETGGHLWADRFDREISDIFAVQDEVTQRIVDALKIKLTPSETARLG
                     AAGTANSKAHDLFLLARSLLFSSSRSREMFARVTAFLDRAIAEDSNYGEAFAGLAMAH
                     ALNWQNHWTEDWSRSLEESKRLAELATGKSPNSTFVRYVASLAYIWSGDHDRFESEIY
                     AALQLSPNDAPSLGTLGFGKTYGGEPLAAIPHIEQAIRLDPLFKQQYVHFLGTAYLVA
                     GDFKAASEQYRERIRLNPKTDLSRAMLAVALGHLGEAEEARQVWRELMEINPKYSFSE
                     HLARLPFRDPADGKRLAEGLRKAGIEA"
BASE COUNT         1082 a         2052 c         2006 g         1111 t
ORIGIN      
        1 aaaatcgcca gcgatgcgcc ttgcgctgaa tggcgactta acttccagcg ctgtggcgcc
       61 acgccgcccg atcgccgtca acccgccata gcgctcccaa cgcccgtatg aacggacacg
      121 cggcggaaag gcgctatggc gtgtcctcca cttcgttccg gcccttcggg tgactgcgat
      181 cggtcggcgt ggcttattgg ctaccaagac gttttgctgt cgaaaccggc cattcggccg
      241 tttcgagcag caaaacgtct tggcggcaaa cggccatcga cgcggaggcg tgtcaaggtt
      301 cgcgaagcga agcggagcga ccgcgaagcg gcttgaccct gacgcgcctc cgcgtcgatg
      361 gcggcgcgtc gttttgagat ttgcgtcatt gcgctccttt aggttcgaaa agccttgaga
      421 gccgcgcctc ctttttctga gtctcgacaa cagaagttcg acgaagaaat ctcgaaacct
      481 tcttgtttcg cgatatcgcc gcctgaggcg actcggttca tgctcatttt ccccggacag
      541 ccctgcgcga agcgaagaga aggggatttc accgcagcgc caagctcggc gccggcgcgg
      601 gcttgagcga gatcggcaag cccgcggcga tgaatagaac ggcgtcgcac gaagcggcca
      661 tggattgatt gagccgccct tgccaatcgc gaaagtcccg tccgagcggc gtttccggca
      721 caattccgag tccgacttcg ttcgacacga agacgaccgg tccctgcaag gcgccggcgg
      781 cgtcgcagag ccgctcgatc tcggccgcaa tatcgctctt ctcgaaagcg agattggcga
      841 gccacagcgt caggcaatcg accagaaccg cgcggccttc gcgcgccgcg ccgagcgcgg
      901 cgacaagatc gcgctcgact tcgagcgtcg tccagccttc gccgcgctcg gcttgatgac
      961 gcttgatccg tgccgccatc tccgcgtcgc cgggcgaagc ggtcgcgatg aacaggcgcg
     1021 aaggcgcggc tttttcgaca agatcgagcg cgtagcggct cttacccgag cgggcgccgc
     1081 ccaggatcaa tacgctgcgg caggcgagcg gcgcggaggc ggtttgcatc aatcttttct
     1141 ggcgccaaag aggcggcgcg gcaatcggcc ttcaaggacc gcgcggccga tgataggaag
     1201 agccgtcgca atcggctttc ccgcatgaat cccatttttg caaatgtcga gaccagcgtc
     1261 ttcgagacca tgtcgcgcct ggcgcgcgag cgacaggcgg tcaatctcgg ccaaggcttt
     1321 cccgacgatc ccggccccgc cgacgtgcgc gccaaggccg cggaggcggt gatcgacggc
     1381 tggaaccaat atccgccgat gatggggctc gccgagctgc gcgaggcggt cgccgcgcat
     1441 tacaagcgct tccaagacct cgatctcgat cccagcgccg agatcatggt cacgtccggc
     1501 gcgaccgaag ccctggccgg cgccttgctc gccttgatcg agccgggcga cgaggtcgtg
     1561 ctgttccagc cgatgtatga cgcctatctg ccgctggtca ggcgcgccgg cggcgttccg
     1621 cgcttcgtca ccttgcggcc gcccgattgg cggatcaggg aatccgatct cgccaaggcg
     1681 ttttccgacc gcacccgcgt cgtccttttc aacaatccgc tcaatccggc aggcgtcgtc
     1741 tacgatcgcg aggcgctcgc tctgctcgcc cggttctgcg tcaggcgcga tgcgatcgcc
     1801 gtctgcgacg aggtctggga gcatgtcctg ttcgacggcc gcgcgcatat tccgctgatc
     1861 gccatggacg gcatgcgcga gcgcaccgtg aagatcggct cggcgggcaa gattttctcg
     1921 ctgaccggct ggaaggtcgg ctttgtctgc gccgcgccgg ctttgatgag ggctttggcc
     1981 aaggcgcatc aattcatcac cttcaccacg ccgcccaatc tgcagagcgc cgtcgcctat
     2041 ggcctcggca aggaggacgg ctatttcttg tcgatgcgcg ccgccttgca acgcagccgc
     2101 gaccgcttca gccgcggcct gaccgagcgc ggctttaccg tcctgcccag cgccggcacg
     2161 tatttcctca atatcgatat cgcgccgctc ggtcgaagcg acgatctcgc cttctgcacc
     2221 gagctcgtca aagagcaagg cgtcgcggcc attccggtct ccgcttttta cgccgaggac
     2281 gcggtcaaga ccgtcgtgcg cttctgcttc gccaagaggg actccactct cgacgacgct
     2341 ttggcgcgtc tcgatgcgat ccggccgcgt tgaggcgccg ccatgaagct tgctctcgcg
     2401 acgatcgcct tggccttctg cgcctcgttc gccgtcgcgg cggagcgatc gcctttcgcg
     2461 ccgccggcca agccgcagcc gcattttatc cacctgctgg cgttcggcga ttatttcgac
     2521 gccaatgccc tggccgaatt cgaaagccgg agcggccggc agatcgctta cgacgcctat
     2581 ggcgcggtga gcgagatcgc tgcgaaattg cgcgacggcc attacgacct cgtcgtgctg
     2641 ccgggcccgg ccttgcgcga ggagatcgcc gcgggcgcgc tgcagaagat cgacgaggcg
     2701 cggctggcga atgccggcgc cgtcgcgccg cgcgtcgcgg ccaagctcga agcctatgac
     2761 ccgtccggcg cctacgccct gccctatatg tggctcgtca ccgggctttt gcttgacgag
     2821 agcaagatcc aggcgcggct cggcgccggg ccggtctcct ggggcttgat cttctcgccc
     2881 gatctggcgc gccgcttcgc cgattgcggc gtcgcgacgc ccaacgatcg cgacaatatg
     2941 ttcatggccg cctggcgcta tatgggcgtc aatccggccc ggctcaacgc gctcgagatc
     3001 aagcgcgccg ccgagattct catccggctc aaaatgggcg cgcgcgcctt ttcgtcgccc
     3061 gatatcgacg gcgcgctggc caacggctcg gcctgcgtca gcgtcggcgc cgagatcgac
     3121 gcgaggcgcg cgatggagcg ggccaagcaa agcggccagc cgatatcggt gcgtttcgtc
     3181 atcccgagag aaggcgcgcc gatgtcgatc gactctttcg cgatccccaa ggatgcgccg
     3241 catcccgatg acgcctatgc gctgctcgat ttcctgctgc gccccgacat cgccgcgcgc
     3301 aacgcccgcg ccaccggcgt tgcgagcggc gatgatggcg gcgacgaaga ggcgttcaag
     3361 cgcctgtcgc cggaaggggc gatcagcccg gctctcgaag cgctggtcga tcaggaatgg
     3421 acgcggatca agggcggcga gaaagagggg gcgagcggca aaaaggccaa tccgcatatc
     3481 ctcacaatcg agccgcgcgg cggcggcaag gctaaacgcg ccccgcgccc gcggtagaat
     3541 tcccctcgcc ccgcggagcg gggagagggt ggccgcgaag cggccgagtg aggggctgcg
     3601 caggaggtac gcgcctttgt acgctcacca gccggctcgg cgcggcccct catccggccg
     3661 cgcttcgcac ggccgccttc tctccgcttg gcggataagg tgtgttaaag aaccttggag
     3721 gcttattttg accatcaccg gctcctgtct ctgcaagcaa atccgctatt cgatcgccgc
     3781 cgcgccgttg atcacgcgcg cctgctggtg ccgcgtctgc caatatttcg ccgccggcaa
     3841 tgcggcgatc agcgcctgct tcagaaggga cgcggtgacg atcgagggcg agaccaagga
     3901 ctatgtcagc atcgccgata gcggcgcggt gatgcatcgc cgcttctgcc cgacctgcgg
     3961 cgtgcatctc ttcagcgaag ccgaatcgcg gccgcatctg atcttcgtgc gcgtcggaac
     4021 gctaaacgat cccgagcttg tccggccgat ggcgaatatc tggaccgcct ccgcgccgtc
     4081 ctgggcctgc atggacgagg gcctgccgcg cttcgaaggc cagccgcctc cggcggggtg
     4141 agcgcagccg cctattcgag gcgccgccgc cggcgcagcc atgctaaggt aaagcgactt
     4201 aagggggggc tatggcggga acccggaaac tcgtcgcgat cctcgccgcc gacgtggtcg
     4261 gcttcagccg gctcgccagc aaggacgagg atcgcacgct ggcgcggctg cgcgcactgc
     4321 gcagcgacct gatcgatccg accgtcgcga tccataacgg acgcgtcttc aagcgcacgg
     4381 gcgacggcgc gctggtcgaa ttccgcagcg tggtcgacgc ggtgcgctgc gcgatcgaag
     4441 tgcaaaacgc gatggtcgag cgcaatgccg gcgtgccgcc ggagcgccgc atcgaattcc
     4501 gcgtaggcat ccatctcggc gatgtcgtcg aggaagccga tggcgatttg atgggcgacg
     4561 gcgtcaatat cgccgcgcgg ctggaaggcg tcgccaaacc cggcgcgatc tgcctctccg
     4621 aggacgctta tcgacaggtc aaggcaaggc tcgacatttc cgtcagcgat cttggcgcga
     4681 cgccgctcaa gaacatcgcc gagccggtcc atgcctatct gcttcaggtc gggcttcctg
     4741 cccaggcgaa ggggggcgcg cccgccctgg ctgaaaaacc ggccggaccg cccgccccgc
     4801 tggacaagcc ctccatcgcc gttctggctt tccagaatat gagcggcgat ccggagcagg
     4861 aatatttcgc cgacgggatc gccgaggacg ttctcaccga cctctccaag atcgggggac
     4921 ttctcgtgat cgcgcgcaat tcgagcttcg cctacaaggg caagccggtc gacatacggg
     4981 cgatcgcgcg cgagctcggc gtgcgttcgg tccttgaggg aagcgttcgg cgcgccggca
     5041 atcgcgtgcg gatcaccgcg cagctcatcg acgccgaaac gggcggccac ttgtgggccg
     5101 accgcttcga ccgtgaaatc agcgacattt tcgcggtcca ggacgaggtc acgcagcgga
     5161 ttgtcgacgc cctgaagatc aagctgaccc cgagcgaaac ggctcggctg ggcgcggcgg
     5221 gaacggcgaa cagcaaagcg catgacctct ttctgctggc gcgctcgttg ctgttctcct
     5281 cttcgagaag ccgcgagatg tttgcgcgcg tgaccgcgtt tcttgatcgg gcgatcgccg
     5341 aggattcgaa ctacggcgag gccttcgccg gcttggcgat ggcccatgcc ttgaactggc
     5401 aaaaccattg gacggaggac tggagccgct cgcttgaaga gtcgaagcgc ctcgcggagt
     5461 tggcgaccgg aaagagcccg aattccacct tcgtgcgcta tgtcgcttcg ctcgcttata
     5521 tctggagcgg cgatcacgac cgatttgaat cggaaatcta cgccgctctt cagctcagtc
     5581 cgaacgatgc tccctccctc gggacgcttg gtttcggcaa aacctatggc ggcgagccgc
     5641 tagccgccat tccgcatatc gagcaggcga tccgtttgga tccgctgttc aagcagcaat
     5701 acgtccactt tctcggtaca gcctatctag tcgcagggga cttcaaggcg gcgtcggagc
     5761 agtacaggga gcgcatccgg ctcaatccga aaaccgatct ttctcgcgcc atgctcgcgg
     5821 tcgcgctcgg ccatttggga gaagccgaag aagcccgtca ggtctggcgc gagctgatgg
     5881 agatcaatcc gaaatactcg ttcagcgagc atctcgcgcg gcttccattc cgcgatccgg
     5941 ccgatggcaa gcgcttggcc gaagggctga ggaaggcggg catcgaagct tgaactaagc
     6001 gtcattcccg cgaaacaggt catgagaaaa cttcgtgacg attcttgatt tgcctcaaaa
     6061 tcgtcgctca gagcgccgaa cgcggcgaaa ttttctgctc cgagggctga aaagcctcga
     6121 aattagacca cgcgccgttt agcgttagaa atcgcgtccc gcgcccggcc aggtgtcggc
     6181 gaggcggtag ccggcggggt acggatcgct cggatcgagc atgagctgat aggtcccggt
     6241 gatccaggcg c
//
LOCUS       DATGJW010000543         4757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_514303, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000543 DATGJW010000000
VERSION     DATGJW010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4757
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_514303"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..831)
                     /locus_tag="VKU03_16450"
     CDS             complement(<1..831)
                     /locus_tag="VKU03_16450"
                     /EC_number="4.1.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012752516.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009228 - thiamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphomethylpyrimidine synthase ThiC"
                     /protein_id="HLJ72907.1"
                     /translation="MNEHVTPKSIQPAPQSVTTGPIAGSRKIYASVKARPDISVPLRE
                     IALSDPAEPPVRVYDPSGPYTEADARIDLANGLRPVREGWIAARGFDAVQGRAVRPED
                     NGNVSDEKLAPHCPAERTLRAGRQGQFVTQFEFARAGIVTEEMIYVAHRENLAREAAL
                     ENAAETLADGESFGAAIPQFVTPEFVRQEVARGRAIIPANINHVELEPMAIGRNFLVK
                     VNANIGNSAVTSGVAEEVEKLVWAIRWGADTVMDLSTGRNIHNIRSWIVRNSPVPIGT
                     V"
     regulatory      complement(897..1003)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1083..2006)
                     /locus_tag="VKU03_16455"
     CDS             complement(1083..2006)
                     /locus_tag="VKU03_16455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016734562.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysR family transcriptional regulator"
                     /protein_id="HLJ72908.1"
                     /translation="MPPPDLNLLIALDALLTESSVAGAARRLGLSASAMSRTLARLRA
                     VTGDPLLAPAGRKMVPTPRAEALRAEVSELSEAVQAVLRPEPSPDIRSLERTFVIRAN
                     DGFVDVFAARLVAAVAAEAPGIRLRFAPKPDKDPRPLREGRIDLDIGVLGETAPEFKT
                     KAMFRDVFIGVARANHPLLTDGPITPERFIAFGHVVASRSGRPHGPVDDALAVLGLSR
                     TIAAVVPSFPAVFSVVSASDLLGLAPRSYFALDHSSETARRGPIVSFALPVRVDGITV
                     SQIWHPRHDADPAHRWLRGLIFDACRRSDSS"
     gene            2144..3541
                     /locus_tag="VKU03_16460"
     CDS             2144..3541
                     /locus_tag="VKU03_16460"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003569799.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HLJ72909.1"
                     /translation="MQRKGGARPLPIDPAGPSADGLAPPRLFLAIGAILAATVLVVLD
                     GAIANVALPSISRALRVSAADCVWVVTSYQLGVVIALLPCAALGESFGGRRIFLAGAA
                     LFTGASMLCALSGSLAELAAARFIQGIGAGAIMALGAMMLRFAFPQRLLGRAIAWNAM
                     VIALSAAAGPGVGSAILAVADWPWLFAVNLPIGALVIAAGQALPRPEGTGRSLDLVSA
                     ALNAGLFAMAVIGADSVATRPALGMALALGAALFLVALVRRERRRAAPLIPIDLLREG
                     SFRLAAIASVCCFSGQMMAIVALPFYFQRELGQSVFSTGLLMTPWPLAVAIAAPISGR
                     LTERVSTAKICAAGGACLALGTALAGAWPIGRDFAPIVVGMILAGAGFGLFQTANNRI
                     LLLSAPKARSGAAGGAQGTARLLGQTIGAVGMALLFEMAPAGAAPRIGLIVAALCALA
                     ASGVSALRMRREGRG"
     gene            3677..4303
                     /locus_tag="VKU03_16465"
     CDS             3677..4303
                     /locus_tag="VKU03_16465"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016846188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HLJ72910.1"
                     /translation="MSEASRQAHWRQVYAGKGEQDVSWFQENPAPSLDMIAQAGAGLD
                     AKIIDIGGGAARLVDALLDKGFRDVAVLDLSEAALAIAKARLGERARAARWIVADATA
                     WRPEPASYDVWHDRAAFHFLVDAADRDAYIARLTEAVKPGGHAIIATFAPDGPEKCSG
                     LPVARYDGDTLAGTIGDGFALIETRRHDHTTPWGSEQRFQFSLFRRRG"
     gene            4351..4617
                     /locus_tag="VKU03_16470"
     CDS             4351..4617
                     /locus_tag="VKU03_16470"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003608524.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HLJ72911.1"
                     /translation="MRFRQKQHVSGDAQRLGASWAMTEGFKEKALRNVVDRLAEFYTP
                     DETSFWLRARHPMLNNERAIDLINQGRTNEVLALIEALDSGTYA"
BASE COUNT          736 a         1555 c         1623 g          843 t
ORIGIN      
        1 caccgtgccg atcggcacag gcgaattgcg gacgatccag gagcggatgt tgtggatgtt
       61 gcggccggtc gaaaggtcca tcaccgtatc ggcgccccag cggatcgccc agaccagctt
      121 ctcgacctct tcggcgacgc cggaggtgac ggcggaattg ccgatattgg cgttgacctt
      181 gaccaggaaa ttgcggccga tcgccatcgg ctccaattcg acgtgattga tgttggccgg
      241 aatgatggcg cggccgcgcg ccacttcctg ccgcacgaat tccggcgtca cgaattgcgg
      301 gatggcggcg ccgaagctct cgccgtcggc gagcgtctca gccgcatttt ccaacgccgc
      361 ctcgcgggcg agattctcgc gatgggcgac atagatcatc tcctcggtga cgatgccggc
      421 gcgggcgaat tcaaattgcg tgacgaactg gccttgcctg ccggcgcgca gcgtgcgctc
      481 ggccggacaa tgcggcgcca gcttttcgtc cgaaacattg ccgttatcct cgggccgcac
      541 ggcgcggccc tggacggcgt cgaagccgcg ggcggcgatc cagccttccc gcaccggccg
      601 taagccattg gcgagatcga tgcgcgcgtc ggcttccgtg tagggaccgg acggatcgta
      661 gacgcggacc ggcggctcgg cgggatcgct cagcgcgatt tcgcgcaatg gcacgctgat
      721 gtctgggcgc gctttgaccg acgcgtaaat tttccgtgag ccggcgatcg gtccggtggt
      781 cacgctctgc ggcgccggct ggatggattt cggcgtgacg tgctcgttca taggctttcc
      841 ttcccgcgtt tgccgccggc cggtctggct tggggatttg ggaaagccga tggtttcggc
      901 ttaaagaacc tcgtccctac gccggcacga cccggatcag gttcaaaggg tccggcgcga
      961 accgccgtct cagccctgga aaggggctcc cctcgagcga gcttgaatct gggcctggag
     1021 gggccgtgag tcaatgggga catatgagag gggcgggaaa agcgggttct gcggcggcca
     1081 tcctaagagc tgtctgaccg gcgacaggca tcgaagatca ggccgcggag ccatcgatgc
     1141 gcggggtcgg cgtcgtgacg cggatgccaa atctgcgaga ccgtgatccc atccacccgg
     1201 accggcaggg caaaactcac gatcggtccg cggcgcgccg tctcgctcga atgatcgaga
     1261 gcaaaatacg atcgcggcgc gagcccgagg aggtcggagg ccgagacgac cgagaagacc
     1321 gctggaaaac tcggcacgac cgccgcgatc gttcgactga gacccaagac ggcgagcgcg
     1381 tcatcgaccg ggccatgcgg ccgaccgctg cgtgacgcca cgacatggcc gaatgcgatg
     1441 aaacgctccg gcgtgatcgg gccgtccgtc aacaaagggt gattggcgcg cgcgacgccg
     1501 atgaagacat cgcgaaacat cgccttggtc ttgaattccg gcgccgtctc gccgagcacg
     1561 ccgatgtcga ggtcgatccg cccctcgcgc aaaggccgtg ggtccttgtc gggtttcggc
     1621 gcgaagcgga gcctgattcc gggcgcctcg gcggcgaccg ctgcgacaag ccgcgccgcg
     1681 aagacatcga cgaatccgtc gttcgcgcga atgacgaagg tccgctcgag gcttcgtatg
     1741 tcggggctcg gttcggggcg caacaccgcc tgaaccgctt cgctcaattc gctcacctca
     1801 gcccgcaagg cttcggcgcg cggggtcgga accatcttcc ggccggccgg cgccaggagc
     1861 gggtcgccag tcactgcgcg cagacgcgcc agcgtgcggc tcatcgccga ggcgctcagg
     1921 ccgagccggc gcgccgcgcc agcgacgctg ctctccgtca acagcgcgtc gagcgcgatc
     1981 agcaggttca agtcgggcgg cggcatggaa gcggcatcag acatggcgtt tcatgcacga
     2041 gtcaagtgcg tccgatgcgc cttccgccag tcctgcaatc ctctatctgt cccccgcagc
     2101 tctggaggtt tccatgtcgg ccgttctctc acgcgaaagc gcaatgcaaa gaaaaggcgg
     2161 cgcccggccg ctgccgatcg acccggcggg gccgtcggcg gacggcctcg ccccgccgcg
     2221 cctattcttg gcgatcggcg cgatcctcgc cgcgaccgtg ctggtcgtcc tcgacggcgc
     2281 gatcgccaat gtcgccttgc cttcgatctc gcgcgccctg cgcgtgtcgg ccgccgattg
     2341 cgtctgggtg gtcacgagct accagctcgg cgtggtgatc gccctgctgc cttgcgcagc
     2401 gctcggcgag agtttcggcg gccggaggat tttccttgcg ggcgcggctt tgtttactgg
     2461 ggcctccatg ctgtgcgcgc tgtcgggcag tctcgccgaa ttggcggcgg ctcggttcat
     2521 ccaggggatc ggcgccggcg cgatcatggc gcttggcgcc atgatgctgc gtttcgcctt
     2581 tccgcagcgt ctgctcggcc gcgcgatcgc ctggaacgcg atggtcattg cgctttccgc
     2641 agcggcggga cctggcgtcg gctcggcgat cctcgccgtc gccgactggc cctggctgtt
     2701 cgcggtgaat ttgccgatcg gcgcgttggt gatcgccgcc ggccaggcgc tcccgcgtcc
     2761 tgaagggacc ggccgatcgc tcgatcttgt cagcgcggcg ctcaacgccg gtctgttcgc
     2821 gatggccgtc atcggagccg acagcgtcgc cacgaggcct gcattgggca tggcgttggc
     2881 gctcggcgcc gctctctttc ttgttgcgct ggtgcgccgc gagagaaggc gcgcggcgcc
     2941 gctcatcccc atcgatctct tgcgcgaggg gtcgttccgc ctggccgcca tcgcctcggt
     3001 ctgctgcttc tcggggcaga tgatggcgat tgtcgcgctg ccattctact tccagcgcga
     3061 gctcggtcag agcgtgttct cgaccggatt gttgatgacg ccatggccgc ttgcggtggc
     3121 gatcgccgcc ccgatttccg gccgtctcac cgaacgcgtc tcaaccgcca agatatgcgc
     3181 ggccggcggc gcctgcctgg cgctcggtac ggcgctggct ggggcctggc cgatcggtcg
     3241 cgattttgcg ccgatcgtcg tcggcatgat cttggccggc gccggattcg gcctcttcca
     3301 gacggctaac aaccgcatct tgctgctgtc tgcgccgaag gcgcgcagcg gcgcggccgg
     3361 cggcgcgcaa ggtacggcgc gcctgctcgg ccagacgatc ggagccgtcg gaatggcgct
     3421 gcttttcgaa atggcgccag ccggcgcggc gccgcggatc gggttgatcg ttgcggcgct
     3481 ctgcgcgctg gccgcgagcg gggtcagcgc gttgcggatg cggcgggagg ggcgagggtg
     3541 aaggaccctg gccgctctcc cggaagaccg ctgtcattga gcgcgcagcg aagagcaatc
     3601 cattcgcagg acgcccgctg tcttcggccg agtccagatg taagctgacc ctcccattgt
     3661 cgcgcgaggc gatcccatga gcgaggcgag ccgtcaggcc cattggcgcc aggtctatgc
     3721 cggcaagggc gagcaggacg tgagctggtt ccaggagaat cccgcgccct cgctcgacat
     3781 gatcgcacag gctggcgccg gcctcgacgc gaagatcatc gacattggcg gcggcgccgc
     3841 gcggctggtc gacgcgctgc tcgacaaagg gtttcgcgac gtcgccgtgc tcgacctttc
     3901 agaggccgcg ctggcgatcg ccaaggcgcg gctcggcgag cgggcgcgcg cggcgcgctg
     3961 gatcgtcgcc gacgcgacgg cctggcggcc cgagcctgcc tcttatgacg tgtggcacga
     4021 ccgcgccgcg tttcatttcc tggtcgacgc cgccgaccgc gacgcctata tcgcccgtct
     4081 caccgaggcc gtgaagcccg gcggccacgc catcatcgca accttcgcgc cggacgggcc
     4141 ggagaagtgc agcggcctgc cggtggcgcg gtacgacggc gacaccctcg ccgggacgat
     4201 cggcgacggg ttcgcgctca tcgagacgcg ccgccatgat cacacgacgc catggggctc
     4261 ggagcagcgg ttccagttca gcctgttccg gcggcgcgga taatgtcggt ccggcttccc
     4321 gtgagcttta caatataaaa tcgagatcaa ttgcgcttcc ggcaaaaaca acatgtcagc
     4381 ggcgatgcac agcgtttggg agcgtcttgg gcaatgacgg aaggcttcaa ggaaaaagcg
     4441 ctccgcaatg tcgtcgaccg actggctgaa ttttacacgc ccgacgagac atcgttttgg
     4501 ctccgcgctc gccatccgat gctcaacaac gagcgcgcca tcgacctcat caatcaaggc
     4561 cggactaacg aagtcctcgc cctcattgag gcgttagatt ctgggactta cgcttgagtg
     4621 gccgaggacg aagagcgcga cctcggattg ctcgactcag gtccgcgcgc cgttcgatta
     4681 attttccaca gagtgtttga aggcgcggct ttctcgccga gcgggattaa atttgaaact
     4741 tccagggggg agcgcaa
//
LOCUS       DATGJW010000544         4260 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1554160, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000544 DATGJW010000000
VERSION     DATGJW010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 4260)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4260)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4260
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1554160"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1101)
                     /locus_tag="VKU03_16475"
     CDS             complement(97..1101)
                     /locus_tag="VKU03_16475"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329373.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HLJ72912.1"
                     /translation="MRMIWTGALVGLAAILAAPAAAQESEFGPANAKETYYWVSNKAN
                     LPLFVQYDYVGMKKIAEELGVKVRVAGPTDFDLPGFIAAVDQVCAQKPAGVSVVGGWD
                     PSLTESVNKCIEMGVPTVVDDGDLPESKRLAYIGTNWTKIGEAQAKVVMAKLPNGGEV
                     AMTSIINAGNMREAVAGFTAYINAKGGGKYKIVANEDDNGDASKAAQVTAALLAAHPK
                     IAAIAGFDSESGAGIVRALQEGGKKPGEVVVTAMEQTPDFFKTAKQGWTQAIVVQNRE
                     LFIYYAVRMLYDYNHNGLKTSGLSAKEGGVPIPPTLDTGVLVVNGDNVDKVLAALKVK
                     "
     gene            complement(1194..2216)
                     /locus_tag="VKU03_16480"
     CDS             complement(1194..2216)
                     /locus_tag="VKU03_16480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329374.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HLJ72913.1"
                     /translation="MMASTLPNTDAAASARAKPVAASIGARAIARLASSRELTLSILI
                     LVLVGAMSAVYPDNFPTRANIAAVLLNAAQNGILVTGMMLLMIAGAFDLSIGSILALT
                     GVWAGVVVGWWNWPAPAGVAVGLVVGATAGLINGLIVTRIGINALIATLATMTIFRGL
                     TYLTAGTGVTPIGDAFKLYGQSVFFGLQTPFWVMLVVVLGGAWAVARTRFFRQFYFIG
                     GNPRAAKLSGIRVQRLTLIGFVIMGGLAGLAGVLGASRLNSAVVSAGIGVELSVITAT
                     VLGGASLKGGEGTVLGGVLGVLFIALVQNAMIINGIGIFWQPVIVGFVLLLAVSLDRF
                     KRTHRS"
     gene            complement(2213..3736)
                     /locus_tag="VKU03_16485"
     CDS             complement(2213..3736)
                     /locus_tag="VKU03_16485"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006329375.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HLJ72914.1"
                     /translation="MRLDVRDVSKSFPGVRALEKVSLTVGSGEIHALVGENGAGKSTL
                     GKIVGGVYEADEGTVWLDGAEMGRVDEAAAGQLGVGMVHQEGSLAPQLSVAENIFAGR
                     QPTRRFGQVDRRRMRESAADLLAQLGVGIDPNAKVADLSPAQGQVVEIAKALSRDLKV
                     LILDEPTAALTLTETQRLFSVVRRLAAGGVSIVYVSHRLAEIFELCDSVTVLKDGRMA
                     GRRDVAATSPDEIIRLMVGRDVRFERNGAAPPKGEIVLEARNVSAAPHVRDASIAVRR
                     GEIVCLAGLIGSGRTEFCEAIFGARKIDEGEVRINGRLLAPTGPWDAIAAGIGMVCED
                     RKESGLFLNMDIVANISAAVLDKVSTGIRVSAAKAEALARRYVEELRVATPSVRQLVG
                     NLSGGNQQKILLAKWLARQPDLLIVDEPTRGVDVGARSEIYRLLRDLAARGMALLVVS
                     SDLPEVLALADRIVVMADGRTVGELSGEGADEESVLRLATRFTKLDQGATQLEKVQG"
     gene            complement(3750..>4260)
                     /locus_tag="VKU03_16490"
     CDS             complement(3750..>4260)
                     /locus_tag="VKU03_16490"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HLJ72915.1"
                     /translation="TILVNNAGISIPGGLEEMTEEEWSATFAVNVGAIHLLGRRVIPM
                     MRAAGGGAIVNIASESAFIGFPMHPAYCASKAAVVHLSRSMAVRYAPDRIRVNALCPG
                     TIDTELYRNFIARQPDPEEAHRRILAMHPLGLGKPEDIAAAAVYLASDEAAYVTGAPM
                     LVDGGATAQ"
BASE COUNT          737 a         1399 c         1392 g          732 t
ORIGIN      
        1 gaggcttcgt ctttcatggg gatgacggca aactcggccc ggcgaggaaa ctgagaaaaa
       61 gcgccgggcc gttttgcgcg cgaatgacgg gtttcgctat ttgaccttca aagcggccag
      121 aaccttgtcg acattgtcgc cattgacgac gagcacgccg gtatcgagcg tcggcgggat
      181 cggcacgccg ccctccttgg cgctcagtcc cgaggttttc aggccgttgt ggttgtagtc
      241 atagagcatg cgcacggcgt aatagatgaa cagctctctg ttctgcacca cgatcgcctg
      301 cgtccagcct tgcttggcgg tcttgaagaa gtcgggcgtc tgctccatcg cggtcacgac
      361 gacctcgccg ggcttctttc cgccttcctg caaggcgcgc acgatgccgg cgcccgattc
      421 cgaatcgaag ccggcgatcg ccgcgatctt gggatgcgcg gcgagcagcg ccgccgtcac
      481 ttgcgccgct ttggaggcgt cgccgttgtc gtcctcattg gcgacgatct tgtactttcc
      541 gccgcccttg gcgttgatat aggcggtgaa gccggcgacg gcctcgcgca tattgccggc
      601 gttgatgatc gaggtcatgg cgacttcgcc gccattcggc agcttggcca tcacgacctt
      661 cgcttgcgct tcgccgatct tcgtccaatt ggtgccgata taggcgaggc gcttggattc
      721 cggcaagtcg ccgtcgtcga ccacggtcgg cacgcccatt tcgatgcact tgttcaccga
      781 ttcggtcagc gaaggatccc agccgccgac caccgaaacg ccggccggtt tctgggcgca
      841 gacttgatcg accgccgcga tgaagcccgg caggtcgaag tcggtgggcc cggcgacgcg
      901 taccttgacg ccgagctcct cggcgatctt tttcatgccg acatagtcgt attggacgaa
      961 cagcggcagg ttggccttgt tcgacaccca atagtaggtt tccttggcgt tcgccggacc
     1021 gaattccgat tcctgcgccg ccgccggcgc cgccaatatc gcggcgagcc cgaccagggc
     1081 cccagtccag atcatacgca tatcgtttcc tccttttttg agccgtgggc tcgcctcggg
     1141 cggctttgcg cgccgccttc ttctcgcgtt gggggcgacc aaccccggaa agatcaggac
     1201 cgatgcgtcc gcttgaagcg gtcgagagag accgccagca acaatacgaa gccgacgatc
     1261 accggctgcc agaagatgcc gatgccgttg atgatcatcg cgttctgcac cagggcgatg
     1321 aacagcacgc cgagcacgcc gccgagcacg gtcccttcgc cgcctttgag gctggcgccg
     1381 cccaggacgg tcgcggtgat caccgaaagc tcgacgccga tcccggccga gaccacggcg
     1441 ctattcagcc gcgacgcgcc gagcacgccg gcaagcccgg caaggccgcc catgatcacg
     1501 aacccgatca aggtcagccg ctggacgcgg atgcccgaca gcttggcggc gcggggattg
     1561 ccgccgatga aatagaattg gcggaagaac cgggtgcgcg cgacggccca ggcgccgcca
     1621 agcacgacga caagcatcac ccagaagggc gtctgcaggc cgaagaagac gctctggccg
     1681 taaagcttga acgcatcgcc gatcggcgtc acgcctgtcc cggcggtgag ataggtgagg
     1741 ccgcggaaaa tcgtcatcgt ggcgagagtc gcgatcagcg cgttgatgcc gatccgcgtc
     1801 acgataaggc cgttgatcag gccggcggtc gcgccgacga ccaggccgac ggcgacgccg
     1861 gcgggggccg gccaattcca ccagccgacc accacgcccg cccatacgcc ggtcaaggcg
     1921 aggatcgacc cgatcgataa atcgaaggcg ccggcgatca tcagcagcat catgccggtg
     1981 accaggatgc cgttctgcgc cgcattgagc agaaccgcgg cgatattggc gcgggtcggg
     2041 aaattgtcgg gatagacggc gctcatcgcg ccgacaagaa cgaggatcag gatggagagg
     2101 gtgagctcgc gcgaactcgc caggcgcgca atcgctcgcg cgccgatcga cgccgcgacc
     2161 ggcttggcgc gcgcgctcgc ggcggcgtcg gtattcggca atgtcgaggc catcatcctt
     2221 ggaccttctc gagttgggtg gcgccttggt cgagcttggt gaaccgcgtc gccaggcgaa
     2281 gcacgctctc ctcgtccgcg ccctctccgg aaagctcgcc gaccgtgcgg ccgtcggcca
     2341 tgacgacgat ccggtcggcc agcgcgagga cctcgggcag atcggacgaa acgaccagca
     2401 gggccatgcc tcgcgcggcg agatcgcgta acaggcgata gatttcggaa cgggcgccga
     2461 catcgacgcc gcgggtcggc tcgtcgacga tgagcaggtc gggctgtcgc gccagccatt
     2521 tggcgagaag aatcttctgc tgattgccgc ccgagagatt gccgaccaat tggcgcaccg
     2581 aaggggtcgc gactctgagc tcctcgacat agcggcgggc gagcgcctcg gctttggccg
     2641 ccgaaacgcg tattccggtc gagaccttgt cgagcaccgc ggcggatatg ttcgccacga
     2701 tgtccatatt gaggaagagg ccgctttcct tgcgatcctc gcagaccatg ccgattccgg
     2761 ccgcgatcgc gtcccagggg ccggtcggcg ccagcagccg gccattgatg cggacctcgc
     2821 cttcgtcgat ctttcgcgcc ccgaatatcg cctcgcagaa ttccgtccgg ccggagccga
     2881 tcaggccggc gaggcagacg atttcgcctc gccggaccgc gatcgaggcg tcgcgcacat
     2941 gcggagccgc cgaaacgttg cgcgcctcga ggacgatttc gcccttcggc ggcgcggcgc
     3001 cattgcgctc aaagcgcacg tcgcggccga ccatcagccg gatgatttcg tcggggctcg
     3061 tcgccgcgac atcgcggcgg ccggccatcc ggccgtcctt taggacggtg acgctgtcgc
     3121 agagctcgaa aatctccgcg aggcggtgcg agacatagac gatcgaaacg ccgcctgcgg
     3181 cgagccgcct gacgacggag aaaagccgtt gcgtctcggt gagcgtcagc gccgctgtcg
     3241 gctcgtcgag aatgagaact ttgaggtcgc gcgaaagcgc cttggcgatc tcgacgacct
     3301 ggccttgcgc aggcgacaga tcggcgactt tggcgttggg gtcgatgccc acgccaagct
     3361 gagcgagcaa atccgcggcg ctctcgcgca tccggcgccg gtcgacttgc ccgaagcgcc
     3421 gcgtcggctg gcgtccggcg aagatattct cagccacgct cagttggggc gcgaggcttc
     3481 cctcctgatg gaccatgccg acgccgagct ggccagccgc agcttcgtcg acccggccca
     3541 tttccgcgcc gtcgagccag accgtccctt cgtcggcttc gtacacgccg ccgacgatct
     3601 ttcccaaagt ggatttgccc gcgccgttct cgccgaccag cgcgtggatt tcgccgctcc
     3661 caacggtgag cgacaccttt tccaaggcgc gcacgcccgg aaagctcttg ctgacgtcgc
     3721 gaacgtccaa ccgcattggc atgctgcgcc tattgagcgg ttgcgccgcc gtcgaccagc
     3781 atcggagcgc cggtgacata ggcggcttcg tctgaggcga ggtacacggc cgccgcggcg
     3841 atgtcctccg gcttgcccag gccaagcgga tgcatggcga ggatgcggcg atgggcctcc
     3901 tccgggtccg gctgccgtgc aatgaaattg cgatagagct cggtgtcgat cgtgccgggg
     3961 caaagcgcat tgacccgaat gcggtcgggc gcgtagcgga ccgccatcga gcgcgacaga
     4021 tgcacaacgg cggctttcga ggcgcaatag gcgggatgca tcggaaagcc gatgaaggcg
     4081 ctctcgctgg cgatgttgac gatcgcgccg ccgccggccg cccgcatcat cgggatgacg
     4141 cggcggccga gaagatggat cgcgccgaca ttcaccgcga atgtggcgct ccattcctct
     4201 tcggtcattt cctcaagacc gccgggaatg gagattcccg cattgttgac gaggatcgtc
//
LOCUS       DATGJW010000545         5510 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_621120, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000545 DATGJW010000000
VERSION     DATGJW010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5510)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5510)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5510
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_621120"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..529)
                     /locus_tag="VKU03_16495"
     CDS             complement(<1..529)
                     /locus_tag="VKU03_16495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018388144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HLJ72916.1"
                     /translation="MLHFENVGLRYGMGSEVVRDLTFSVEAQSFQFLSGPSGAGKTTL
                     LRLMLLSLKPTRGIIRIFGRDADSLDKDAITSLRRRIGVVFQDFRLLDHLTTYENVAL
                     PLRVQGRSEASYRAEVVELLKWVGLGERMNALPPVLSGGEKQRAAIARALIARPELLL
                     ADEPTGNVDPSLARRL"
     gene            719..1285
                     /locus_tag="VKU03_16500"
     CDS             719..1285
                     /locus_tag="VKU03_16500"
                     /inference="COORDINATES: protein motif:HMM:NF023334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3426 domain-containing protein"
                     /protein_id="HLJ72917.1"
                     /translation="MFAACGNCGRQFESGGGVRFCPRCGGSAAADGFFRRRVRAAAPV
                     LALGLALAGAMGAVGARDRLVRWVPATAPAYAAIGLPVNLRGIAIDGVGASIVETAGR
                     RVLTVEGSMVNLRGQKAEIPDMRIALRDANAHEIYVWTARPPKAALDPREETSFRLRL
                     ASPPAAAHDVLVRFASAIEKSAPAGDKL"
     gene            1279..1821
                     /gene="hpt"
                     /locus_tag="VKU03_16505"
     CDS             1279..1821
                     /gene="hpt"
                     /locus_tag="VKU03_16505"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012320927.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HLJ72918.1"
                     /translation="MVSGERQVQVLIDEKAIAERTQKLADEIAAAKPKDLLVVAILKG
                     SFMFAADLLRAMHRAGLDPHVEFVHLASYFEGTVSTGSVRILKDIESSVKDRDVLLVD
                     DILESGRTLAFAKDLLAARGARRVMVCAMLEKPGKRAVQISADFVGFVCPDVFVVGYG
                     MDVAHSYRQLPFIGRVVTGE"
     gene            1971..3785
                     /locus_tag="VKU03_16510"
     CDS             1971..3785
                     /locus_tag="VKU03_16510"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019402718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family protein"
                     /protein_id="HLJ72919.1"
                     /translation="MFQSLYQSFDDVADPSLGAPRVKALREELARRDLAGFIVPRADQ
                     HQNEYVPASEERLRWLTGFSGSAGLAIILADRAALFVDGRYTLQAAEQVDAKLFEIRH
                     VVDDPPAAWIERHLGGGKLGYDPWLHTPDAVERFSRAAAAAAGELVAAEPNPIDAIWT
                     DRPAPPLGAVRMHKMSLAGESARSKLKKVQAKLKSDGLVVSDAHDVAWLFNIRGSDVA
                     HTPLALSYAFVPRAGKPKLYIDGRKLSNRVRAALSVLAEIGEERELVADLKALGAKRA
                     RLAFDAASAAALLAQTLKEAGGAPEIGADPIALLKAAKNPAELAGACAANLRDGAALT
                     RFLAWFDREAPKGRLTEIAAAEALESFRRDTGLLKDVSFPSISAAGPNSAIPHYRVTT
                     ASNRTIDKGIFLIDSGGQYEDGTTDITRTIAVGAPTKEMRDRFTRVLKGHIAIACAVF
                     PKGVAGAQIDAFARKALWEAGLDFDHGTGHGIGAYLSVHEGPQRIAKTGATALEPGMI
                     VSNEPGYYAAGRYGVRIENLLVVEEREIAGAERKMLGFATISLAPIDLRLIEPKLLTK
                     DERDWLDAYHARVRKELAPLVDAPTRAWLKKATRRLAG"
     gene            complement(3888..5495)
                     /gene="pgi"
                     /locus_tag="VKU03_16515"
     CDS             complement(3888..5495)
                     /gene="pgi"
                     /locus_tag="VKU03_16515"
                     /EC_number="5.3.1.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018234955.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004347 - glucose-6-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-6-phosphate isomerase"
                     /protein_id="HLJ72920.1"
                     /translation="MQALSRQVLARSVADEFAADPERFESMHVVLDDLLFDYSKQRLN
                     KAVLAELVALAAACEVEEKRDAMFRGDPINVTERRAALHTALRNLSGDPVFVGGRDVM
                     PDVIAERAKVEAFASDVREGRLRGALGQPMTDIVNIGIGGSDLGPAMATKALSPYAAP
                     NLRSHFVSNVDGADIADTLRGLDPSRTLFIVSSKTFTTQETMTNAASARSWIAGALGD
                     AAVGDHFAAVSTRLDKVAEFGIEAGRVFGFWDWVGGRYSIWSSIGLPLTIAIGPQHFE
                     EFLAGGYDIDRHFRAAPIAENIPILMGLISVWNRDILGHASQAVIPYDQRLSRFPAYL
                     QQLQMESNGKSVRLDGAPASDATGAVVWGEPGTNGQHAFFQLIHQGTEIVPVDFLVAA
                     RPTNADGRHHDLLVANCFAQSEALMRGRTTAQADAINRGLGLSAEEAARLAPHKTFSG
                     SRPSSTLLYDKLTPRMLGRLIALYEHKVFVEAAIWGINPFDQWGVELGKELASRLAPI
                     VSDAGADLSKLDASTAGLVAHLRKLRK"
BASE COUNT          896 a         1848 c         1873 g          893 t
ORIGIN      
        1 tcaggcggcg tgccaggctc ggatcgacgt tgccggtcgg ctcgtcggcg agcagaagtt
       61 cgggccgggc aatcagcgcc cgggcgatgg cggcgcgctg cttttcgccg cccgacagga
      121 ctggcggcag cgcgttcatg cgctcgccga ggccgaccca tttgaggagc tccaccacct
      181 cggcgcgata gctcgcctca ctgcggccct gcacgcgcaa gggcagggcg acgttctcgt
      241 aggtggtcag gtggtcgagc agccgaaaat cctggaacac cacgccgatg cgccgccgca
      301 gtgaggtgat cgcgtccttg tcgagactgt ccgcgtcgcg gccgaaaatc cggatgatgc
      361 cgcgggtcgg cttcagcgac agcagcatga ggcgcagcag cgtcgtcttg ccggcgccgg
      421 atgggccgct gaggaattgg aagctctgcg cttcgaccga aaaggtgagg tcgcgcacga
      481 cctccgatcc cattccgtat cgtaagccca cattttcgaa atggagcaag gcgcgactca
      541 aagctctggc gggtcagaaa gagccgaacc tgctccttgc gtcaaatgtg aggaaaagcg
      601 ccgcggcgcc ggcagaaatc ggggcaaaaa gcgccgccgg ccttaaccgc gccttaacca
      661 tgattgcgcc caatggcggc gacggctctt ttcgccggac ttctcaatag gagcagccat
      721 gtttgccgct tgcgggaact gcggccggca gttcgaaagc ggcggcggcg tccgtttttg
      781 cccccgttgc ggcggcagcg cggcggcgga cggattcttc cgacggcggg tccgcgcggc
      841 ggcgcccgtc ctcgccctcg gcctggcgct ggccggggcc atgggcgcgg tcggcgcccg
      901 agacaggctc gtccgctggg tcccggcgac ggcgccggcc tatgcggcga tcggcctgcc
      961 ggtcaatctc aggggcatcg ccattgacgg cgtcggcgcc agcattgtcg agacggccgg
     1021 ccggcgggtg ttgaccgtcg agggctcgat ggtcaactta cggggtcaaa aggcggagat
     1081 cccggatatg cgcatcgcgt tgcgcgacgc caacgcccac gaaatctatg tctggacggc
     1141 gcggccgccc aaggcggcgc tcgatccgcg ggaggagacc tcgttccggc tgcgcctcgc
     1201 ctcgccgccg gccgccgcgc acgacgtcct cgttcgtttc gcctcggcca tcgagaaaag
     1261 cgcgccggcc ggggacaaat tgtgagcggc gagcgccagg tccaggttct catcgacgaa
     1321 aaggcgatcg cggagcggac gcaaaagctc gccgacgaga tcgccgccgc caagccgaag
     1381 gacttgctgg tcgtcgcgat cctcaaaggc tcgttcatgt tcgccgccga cctgctcagg
     1441 gcgatgcacc gggccggcct cgacccgcat gtcgaattcg tgcacttggc gagctatttc
     1501 gaggggacgg tttcgaccgg ctcggtcaga atcctcaagg acatcgaatc ctcggtcaag
     1561 gaccgcgacg ttctgctcgt cgacgacatt ctcgaatcgg gccgcaccct cgccttcgcc
     1621 aaggaccttc tggcggcgcg cggcgcgcgg cgggtgatgg tctgcgcgat gctcgaaaag
     1681 ccgggcaagc gagccgtgca gatctccgcc gacttcgtcg gctttgtctg ccccgacgtc
     1741 ttcgtcgtcg gctatggcat ggacgtcgcc cattcctacc ggcaattgcc gttcatcggg
     1801 cgcgtggtga caggggagta ggcgggttta ccgacttgct ctgcggcgta ccctcacccc
     1861 agccctctcc cgcaagcggg agaggggggc cgcgaagcgg ccggatgagg gtaagtcgcg
     1921 gcgttcattg ttcgccggcg atcgcgtgcg atgttgtgag gcgttctcct atgttccaaa
     1981 gcttgtacca atcgttcgac gatgtcgctg acccttctct cggcgcgccg cgcgtcaagg
     2041 cgctgcgcga agaattggcg cgtcgcgacc tggccggctt catcgtcccg cgcgccgacc
     2101 agcatcagaa cgaatatgtg ccggcttcgg aagagcgcct gcgctggctg accggctttt
     2161 ccggctcggc cgggctcgcg attatccttg ccgaccgcgc cgcgctgttc gtcgacggac
     2221 gctacacgct gcaggcggcc gagcaggtcg acgccaagct tttcgaaatc cgccacgtcg
     2281 tcgacgatcc ccccgccgca tggatcgagc gccatctcgg cggcggcaag ctcggctacg
     2341 atccctggct gcacacgccc gacgcggtcg agcgcttctc gcgcgccgcc gctgccgccg
     2401 ctggcgagct cgtcgcggcc gagccgaatc cgatcgatgc gatctggaca gaccggccgg
     2461 cgccgccgct cggcgcggtt cgcatgcaca agatgagcct tgccggcgaa agcgcgcggt
     2521 cgaagctcaa aaaagttcag gcgaagctga agtccgacgg ccttgtcgtc tccgacgccc
     2581 atgacgtcgc ctggctgttc aatattcggg gaagcgacgt cgcccatacg ccgctggcgc
     2641 tgtcctatgc tttcgtgccg cgcgccggca agccgaaact ctatattgac ggccgcaagc
     2701 tctccaatcg cgtcagggcc gccctctcgg tcctcgccga aatcggcgag gagcgcgagc
     2761 tcgtcgccga tctcaaggcg ctcggcgcta agcgcgcgcg cctcgccttc gacgcggcga
     2821 gcgccgccgc gcttcttgcg cagaccctga aggaggctgg cggcgcgccg gaaatcggcg
     2881 ccgatccgat cgcgcttttg aaggcggcga agaatcccgc cgaactggcc ggcgcctgcg
     2941 ccgccaattt gcgcgacggc gccgcgctga cccgttttct cgcctggttc gaccgcgaag
     3001 cgccgaaagg ccgcctgacc gagattgcgg cggccgaggc gctggaatcg ttccgccgcg
     3061 acaccggcct gctgaaggac gtctcgtttc cctcgatctc cgccgccggc ccgaattcgg
     3121 cgattccgca ttatcgcgtc accacggcgt cgaaccgaac gatcgacaag ggaattttcc
     3181 tcatcgacag cggcggtcaa tacgaggacg gcacgaccga catcacccgc acgatcgcgg
     3241 tcggcgcccc gaccaaggaa atgcgcgacc gcttcacccg cgtgctgaaa ggccacatcg
     3301 ccatcgcttg cgccgtcttt ccgaagggcg tcgccggcgc gcagatcgac gctttcgcgc
     3361 gcaaggcgct gtgggaggcc gggctcgatt tcgaccatgg aacggggcac ggaatcggcg
     3421 cctacctctc ggtgcatgaa gggccgcagc gcatcgccaa gaccggcgcg acggcgctcg
     3481 agccgggcat gatcgtctcc aacgagcctg gctattacgc cgccggccgc tacggcgtcc
     3541 gcatcgagaa tctgctcgtc gtcgaggagc gcgagatcgc cggggccgag cgcaagatgc
     3601 tgggctttgc gaccatctcg ctcgcgccga tcgacctgcg gctgatcgag ccgaaactgc
     3661 tgacgaaaga cgagcgggat tggctcgacg cgtaccatgc gcgggtgcgc aaagagttgg
     3721 cgccgctggt cgacgcgccg acgcgagcgt ggctgaagaa agcgacgcgg cggctcgcag
     3781 gataatcaat gttgcagacg gcgcgcgcgc cgcgagatgt gagcgcgacg gctgcaggat
     3841 gagcacggcg tgcgggcgac gccgagcctc gtcctcggac gcactcgtta tttccttagc
     3901 ttgcgcaaat gcgccaccag ccccgccgtc gacgcgtcga gcttggaaag atcggctccg
     3961 gcgtcggaga cgatcggcgc gaggcgcgag gccagctcct tgccgagctc gacgccccat
     4021 tgatcgaacg ggttgatgcc ccagatcgcc gcctcgacga agactttgtg ctcgtagagc
     4081 gcgatcagcc ggccgagcat gcgcggcgtc agcttgtcgt aaagcaatgt gctcgacggc
     4141 cggctgccgg agaacgtctt gtgcggcgcg agccgcgccg cctcctccgc cgaaaggccg
     4201 aggccgcgat tgatcgcgtc ggcttgcgcc gtcgtgcggc cgcgcatcaa ggcttcgctc
     4261 tgcgcgaagc aattggcgac gagcagatcg tggtggcggc cgtcggcgtt ggtcggccgc
     4321 gccgcgacga ggaagtccac cggcacgatt tccgtgccct gatggatcag ttggaagaaa
     4381 gcgtgctggc cattcgtgcc gggctcgccc cagaccaccg cgccagtggc gtcggaagcg
     4441 ggagcgccgt cgaggcggac gctcttgccg ttgctctcca tctgcaattg ctggagatag
     4501 gccggaaagc gcgacaggcg ctggtcgtac ggaatcaccg cctggctggc gtggccgaga
     4561 atgtcgcggt tccagacgct gatcagcccc atcaggatcg gaatgttttc cgcgatcggc
     4621 gcggcgcgga aatggcggtc gatgtcgtag ccgccggcga ggaattcctc gaaatgctgc
     4681 ggcccgatcg cgatcgtcag cggcaggccg atgctcgacc agatcgaata gcggccgccg
     4741 acccagtccc agaagccgaa gacgcggccg gcctcgatgc cgaattcggc gaccttgtcg
     4801 agccgggtcg acaccgccgc gaaatggtcg ccgaccgccg cgtcgcccag cgcgccggcg
     4861 atccagctcc gcgcgctcgc cgcattggtc atcgtctcct gcgtcgtgaa cgtcttggac
     4921 gaaacgatga acagggtgcg cgacgggtcg aggccgcgca gcgtgtcggc gatgtcggcg
     4981 ccgtcgacat tggagacgaa atggctgcgc agattcggcg cggcgtaggg cgacagcgcc
     5041 ttggtcgcca tggccgggcc cagatccgag ccgccgatgc cgatattgac gatgtcggtc
     5101 atcggctggc cgagcgcgcc gcgcaagcgc ccctcgcgca cgtcgctggc gaacgcctcg
     5161 accttggcgc gctcggcgat cacgtcgggc atgacgtcgc ggccgccgac aaagaccgga
     5221 tcgccggaaa ggttgcgcaa ggcggtatgc agcgcggcgc ggcgctcggt gacgttgatc
     5281 gggtcgccgc ggaacatcgc gtcgcgcttc tcttcgacct cgcaggccgc cgccagcgcg
     5341 acgagctcgg ccaagacggc cttgttcagc cgctgcttgg aatagtcgaa gagcagatcg
     5401 tcgagcacga catgcatgct ctcgaaacgc tccggatcgg cggcgaactc gtcggcgacg
     5461 ctccgcgcca ggacctgccg gctcagcgct tgcagagcgc tcttgcgcgc
//
LOCUS       DATGJW010000546         5796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_726117, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000546 DATGJW010000000
VERSION     DATGJW010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5796
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_726117"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            26..1405
                     /locus_tag="VKU03_16520"
     CDS             26..1405
                     /locus_tag="VKU03_16520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015926969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AarF/UbiB family protein"
                     /protein_id="HLJ72921.1"
                     /translation="MQDNEANRFSARAARYARVGANVGAFAARMASNRLRGAEDPRSQ
                     AQALAGALGSLKGPLMKVAQMLATIPDALPPDYAEELMRLQAEAPPMGAAFVKRRMQA
                     ELGPDWRKSFAEFDLHPAAAASLGQVHRAVALDGERVACKLQYPDMASAVEADLAELD
                     LLFSIHRRMGPAIDTREIAAEIGARIREELDYRREAKLARLYRLMLADRPAVRVPRVR
                     AELSTRRLLTMEWLEGDKLTKFEKAPQSARNAIALALFEAWWRPFSQYGVIHGDPHLG
                     NYTIAAKGDEIAGLNLFDYGCVRIFPASFVGGVVDLYQGLKTKDEARIVHAYETWGFV
                     KLKRETIDALNIWARFIYGPLIDDRVRTIADGVKPGHYGRREAFTVHQALKAQGPLTV
                     PREFVFMDRAAIGLGGVFLRLGAALNFHRLFEQAIEGFAVEEVAKRQAAALDAAELGE
                     ESPRFAQRG"
     gene            1605..3035
                     /locus_tag="VKU03_16525"
     CDS             1605..3035
                     /locus_tag="VKU03_16525"
                     /inference="COORDINATES: protein motif:HMM:NF013584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amidase family protein"
                     /protein_id="HLJ72922.1"
                     /translation="MTDLLLEPAAALLEKLRARKLSSRDLTELTLARIASLNPKINAF
                     CAVDEASARQAAARADSRLAQGEARPLEGLPIGIKDVFDTVGFTASAGAPAYKDRRPE
                     QDAPAVARLRAAGAIILGKTNVPVFASDFQAFNPIHGATNHPWKADFSPGGSSGGSAA
                     SLASGMSALELASDLGGSIRWPAHCCGLYGLKTSWGLVTSYGHIPPPPDKRTERDADC
                     LVVGPMARSAGDLELMLKIIAGPRHAERPVSLRPARARSAKGLRVALWASDPFAPADK
                     SVRDKVEEAARRLAALGAIIDDRARPAFPFSEAFEVFALYNHAVIGWGLPGPLRDKIA
                     ATAPRYKRGDLSHRALQARGVRITPGEYRDLELRRRKLQAHWARFFERCDVVLCPPAP
                     VGPIRHDHAPDIHNRKLIVDGEERPYLDFLVWSSLASGAGLPAAVAPVGRGPDNLPRG
                     VQIIAAMGEDLTATAVAAMIGAAEGL"
     gene            complement(3142..3891)
                     /locus_tag="VKU03_16530"
     CDS             complement(3142..3891)
                     /locus_tag="VKU03_16530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012334629.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72923.1"
                     /translation="MSSMQRYVGRGVIVTGGASGIGLKAAERFASEGAAVALWDADQG
                     RLDAAKASLGPKAHIFRLDVANPVAVEEAARAANEALGRIDALVCSAGIAGKNALVVD
                     YPIEEWKRVLDINLNGLFYCNRYVAPYMTKRGYGRIVNVASIAGKEGNPTASAYSASK
                     AAVIGLTKSLGKELAKTGVTVNCITPATVDTPILAQVSQAHIDYMRSKIPMERFGTVD
                     EMAAIICWLASEECSFTTGAAFDVSGGRATY"
     gene            4044..5255
                     /locus_tag="VKU03_16535"
     CDS             4044..5255
                     /locus_tag="VKU03_16535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018237367.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CaiB/BaiF CoA-transferase family protein"
                     /protein_id="HLJ72924.1"
                     /translation="MKPLEGLKVLELARILAGPWAGQTLADLGAEVIKVERPGAGDDT
                     RGWGPPFVAGADGGRGDAAYYHACNRGKRSVAVDFERPEGRDIVRRLAARADVVIENF
                     KTGGLAKYGLDYPSLKALNPRLVYCSITGFGQTGPYAKRAGYDFMIQGMGGIMDLTGE
                     PDGEPQKPGVAYADIFCGLYSVIAIQAALARRAATGEGASIDMALMDSQVAVLANQAL
                     NFLVSGVAPRRLGNAHPNLVPYQVFKVADGHIIIATGNDRQTRDLCRVLGVEELADDP
                     RFLRNADRIAHREAFIALLETRTRTFRAAELLAALEKAVVPAGPIKSVAEVFADPQVQ
                     ARGMRIELESKAAAAGTVPNVRAPILLDGEAMIPVRAAPALGEDTGAVLAELGLGEAE
                     IAALRAKGVVG"
     gene            complement(5324..5770)
                     /locus_tag="VKU03_16540"
     CDS             complement(5324..5770)
                     /locus_tag="VKU03_16540"
                     /inference="COORDINATES: protein motif:HMM:NF025063.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="integrase core domain-containing protein"
                     /protein_id="HLJ72925.1"
                     /translation="MAPPARTLREQLERLRRFQRLYNEERPHEALGNKTPAKHYAVSP
                     RRFDGVLREPNYDAEHTVRRVRHNGEIRWQGATIYISGALAGEPIGLVENDDGAWLVS
                     YGPILLGVIVHRGDRLRALPPRVCGLVDNAARCPQGPQTKQQQRRA"
BASE COUNT          881 a         1994 c         1964 g          957 t
ORIGIN      
        1 cccccgtctt cgacggggga ggataatgca agacaacgaa gccaaccgtt tctcggcgcg
       61 cgccgcgcgc tatgcgcggg tcggcgccaa tgtcggcgcc ttcgccgcgc gcatggcgtc
      121 gaaccggctg cgcggcgccg aggatccgcg cagccaggcg caggcgctgg ccggcgcgct
      181 cggctcgctg aaagggccgc tgatgaaggt cgcccagatg ctggcgacca ttcccgacgc
      241 gctgccgccc gactacgcgg aagaattgat gcggctgcaa gccgaagcgc cgccgatggg
      301 cgcggccttc gtcaagcgcc gcatgcaggc cgagctcggg ccggactggc gcaaaagctt
      361 cgccgaattc gatctccatc ccgccgccgc cgcctcgctc ggccaggtcc atcgcgccgt
      421 cgcgctggac ggcgagcgcg tcgcctgcaa attgcaatat cccgatatgg cctcggcggt
      481 cgaagcggac cttgccgagc tcgacctgct cttttcgatc catcgccgca tggggccggc
      541 gatcgacacg cgcgaaatcg ccgccgagat cggcgcgcgc atccgcgagg agctcgacta
      601 ccgccgcgag gccaagctgg cgcggcttta ccgtttgatg ctggccgatc ggccggccgt
      661 gcgcgtgccg cgcgtgcgcg ccgaattgtc gacgcgccgc ctgctgacca tggaatggct
      721 ggaaggcgac aagctgacga aattcgagaa ggcgccgcaa agcgcgcgca acgccatcgc
      781 gctcgctctg ttcgaggcgt ggtggcggcc gttctcgcaa tacggcgtca ttcacggcga
      841 tccgcatctc ggcaattaca cgatcgcggc gaaaggcgac gagatcgccg gcctcaatct
      901 gttcgattac ggctgcgtca ggattttccc ggcgagcttt gtcggcggcg tcgtcgattt
      961 gtaccaagga ctcaagacga aggacgaggc gcggatcgtc cacgcctacg agacctgggg
     1021 cttcgtgaaa ttgaagcgcg agacgatcga cgcgctcaac atctgggcgc gcttcatcta
     1081 tgggccgctg atcgacgatc gcgtccgcac catcgccgac ggcgtcaagc ccggccatta
     1141 cggccggcgc gaggcgttca cggtgcatca ggcgttgaag gcgcaaggcc cgctgaccgt
     1201 gccgcgcgaa ttcgtcttca tggatcgcgc cgcgatcggc ctcggcggcg tgttcctgcg
     1261 gctcggcgcg gcgctgaatt tccaccggct gttcgagcag gcgatcgagg gcttcgccgt
     1321 cgaggaggtc gcgaagcgac aggcggcggc gctcgacgcg gccgagctcg gcgaagaatc
     1381 ccctcgcttc gcgcagcggg gatagggtgg cggccgggta ggctgcgcgg agcctgccgg
     1441 ttagcgtctc ggagcgcgta cctttcgagt gcggcgccgt ccctcatccg gcgcttcgcg
     1501 ccgccttctt cccgctttgc cgggagaaag ggtttcgacc caagcgggac cgggttgctt
     1561 tttgcgctgg cgcgggcgag cctacgatcg agctgggata gttcatgact gatctgctcc
     1621 tcgagcccgc agccgcgctc ctcgaaaagt tgcgggcgcg aaaattgtcg agccgcgacc
     1681 tgacggagct gacgctcgcg cgcatcgcga gcctcaatcc caagatcaac gccttttgcg
     1741 ccgtcgacga agcgtcggcg cgccaggcgg cggcgcgggc cgactcgcgt ttggcccaag
     1801 gcgaggcgcg gccgctcgaa ggcctgccga tcgggatcaa ggacgtgttc gacacggtcg
     1861 gcttcaccgc cagcgccggc gcgccggcgt acaaggaccg ccggcccgaa caggatgcgc
     1921 cggccgtcgc gcggcttcgc gccgccggcg cgatcatcct cggcaagacc aacgtcccgg
     1981 tcttcgcctc ggattttcag gccttcaatc cgatccatgg cgcgaccaat catccgtgga
     2041 aagcggattt ttcgcccggc ggctcgtcgg gcggctcggc cgcttccttg gcgagcggca
     2101 tgtcggctct ggagctcgcc tccgatctcg gcggctcgat ccgctggccg gcccattgct
     2161 gcgggcttta cggcctgaag accagttggg gcctcgtcac tagctacggc catatcccgc
     2221 cgccgcccga caagcgcacc gagcgcgacg ccgattgcct cgttgtcggg ccgatggcgc
     2281 gctcggccgg cgatctcgaa ttgatgctga aaatcatcgc cggcccgcgc catgccgagc
     2341 ggccggtttc cttgcggccc gcccgcgcgc ggagcgccaa aggcctgcgc gtcgcgctct
     2401 gggcgagcga tcctttcgcg ccggcggaca aaagcgtgcg cgacaaggtc gaggaggcgg
     2461 cgcgccgcct cgccgcgctc ggcgcgatca tcgacgatcg cgcgcggccg gctttcccgt
     2521 tcagcgaggc gttcgaagtc ttcgcgctct acaatcacgc ggtgatcggc tggggcctgc
     2581 cggggccttt gcgcgacaag atcgccgcga cggcgccgcg ctacaagcgc ggcgatctct
     2641 ctcatcgcgc gctgcaggcg cgcggcgtgc gcatcacgcc cggcgagtat cgcgacctcg
     2701 aactcaggcg gcgcaagctg caagcccatt gggcgcgttt tttcgagcgc tgcgacgtgg
     2761 tcctttgtcc gccggcgccg gtcgggccga tccgccacga ccacgcgccc gatattcata
     2821 accgcaagct gatcgtcgac ggggaggagc gtccgtacct cgatttcctc gtctggtcgt
     2881 cgctggcgag cggcgccgga ttgccggcgg cggtcgcccc ggtcgggcga gggccggaca
     2941 atctgccgcg cggcgtccag atcatcgccg cgatgggcga agacctcacc gcaaccgccg
     3001 tcgccgcgat gatcggcgcg gccgagggtt tgtagccgcg gtcggcgacc gcggctacag
     3061 cgcggcggcg taaaatcgcc ggcgccgcgc ttgccatcgc cgcgctcggc gccaagccca
     3121 agccgcttcg cgttttgtac cttaatacgt cgcccgtccc cccgacacgt cgaacgccgc
     3181 gcccgtcgtg aacgagcatt cctcgctcgc cagccagcag atgatcgcgg ccatctcgtc
     3241 gaccgtgccg aagcgctcca tcgggatttt cgagcgcata tagtcgatat gggcctggct
     3301 gacctgggcg aggatcggcg tatcgaccgt cgccggcgtg atgcaattga cggtgacgcc
     3361 ggtcttggcg agctccttgc cgagcgactt ggtgaggccg atcaccgccg ccttggaggc
     3421 cgaataggcc gaggcggtcg gattgccttc cttgcccgcg atcgaggcga cgttgacgat
     3481 gcggccatag ccgcgcttgg tcatgtaggg cgcgacatag cgattgcagt agaacagccc
     3541 gttgagattg atgtcgagga cgcgcttcca ctcctcgatc ggatagtcga cgacgagcgc
     3601 gttcttgccg gcgattcccg ccgagcagac cagcgcgtcg atccggccca acgcctcatt
     3661 ggcggcgcgc gccgcctcct cgaccgcgac cggattggcg acgtcgagcc ggaaaatatg
     3721 cgctttcggc ccgagcgagg ctttggccgc gtcgagcctg ccctggtcgg cgtcccaaag
     3781 cgcgaccgcc gcgccttcgc tggcgaaacg ctcggccgcc ttgagcccga tccccgacgc
     3841 gccgcctgtg acaatgacgc cgcggccgac ataacgttgc atcgaggaca taggacgttt
     3901 ccttcagggt tgagaaaggg cgccagctta ccggctgcgc ggccgctcgt cacggctccg
     3961 ggttgacgcc gccctcgccc gagacgacaa gctcggccgc gcgggccgct gcgccggccg
     4021 gacgacattc cggggacttg ccgttgaaac cgctcgaagg cctcaaagta ctcgaactcg
     4081 cccgcattct cgccgggccc tgggcgggcc agacgctcgc cgatctcggc gccgaggtga
     4141 tcaaggtcga gcggccgggg gcgggcgacg acacgcgcgg ctggggcccg cctttcgtcg
     4201 ccggcgccga tggcggccgc ggcgacgcgg cctattacca tgcctgcaat cgcggcaagc
     4261 gctcggtggc ggtcgacttc gagcggccgg aagggcggga catcgtccgc cggctggccg
     4321 cccgcgccga cgtcgtgatc gagaatttca agaccggcgg cctcgccaaa tacggcctcg
     4381 attatccgag cctgaaggcg ctcaacccgc gcctcgtcta ttgctcgatc accggcttcg
     4441 gccagaccgg cccctacgcc aagcgcgccg gctacgattt catgatccag ggcatgggcg
     4501 ggattatgga tttgaccggc gagccggacg gcgaaccgca gaagcccggc gtcgcctatg
     4561 ccgacatttt ctgcggcctc tattcggtga tcgcgatcca ggctgctttg gcgcgccgcg
     4621 ccgcgaccgg cgagggcgcc tcgatcgaca tggcgctgat ggattcgcag gtcgcggtcc
     4681 tcgccaacca ggcgctgaat ttcctcgtct ccggcgttgc gccgcgccgg ctcggcaacg
     4741 ctcatcccaa tctcgttccg taccaggtct tcaaggtcgc cgacggccat atcatcatcg
     4801 cgaccggcaa tgaccgccag acgcgcgatc tttgccgcgt gctcggcgtc gaggagctcg
     4861 ccgacgaccc gcgcttcctg cgcaacgccg accgcatcgc tcatcgcgaa gcgtttatcg
     4921 cgctgctcga aacgcgtacg cgaacctttc gcgcggccga gctcctggcg gctctggaaa
     4981 aggcggtggt gccagccggg cccattaagt cggtcgcgga agtcttcgcc gacccgcaag
     5041 tgcaagcgcg cggcatgcgg atcgaattgg aatcgaaagc cgcggcggcg gggacggtcc
     5101 cgaatgtacg cgcgccgatt ctgctcgacg gcgaagcgat gatcccggtc cgcgccgcgc
     5161 cggcgctcgg cgaggacacc ggcgccgtgc tcgcagagct tggcctcggc gaggccgaga
     5221 tcgcggcgct tcgcgcgaaa ggcgtggtgg gataactcct gagcaaccgg caacataggt
     5281 aacacttctg accggggaca tgggtgacat tttctcccct cgttcaggcc cgccgctgtt
     5341 gctgtttcgt ctgtgggccc tgtgggcaac gcgcagcgtt gtccacaagt ccacagacgc
     5401 gcggcggcag ggcgcgcagg cgatccccgc gatggacgat gacgccgaga aggattggtc
     5461 catacgagac gagccaggcg ccgtcgtcgt tttcgacgag gccgatcggc tcgccggcca
     5521 gcgcgccgct gatgtagatc gtggcgcctt gccagcggat ttcgccgtta tggcgcacgc
     5581 gcctgaccgt atgctcggcg tcgtagttcg gctcgcgcaa gacgccgtcg aagcggcgcg
     5641 gcgagacggc gtaatgtttg gccggcgttt tgttgccaag cgcttcgtgc ggccgctctt
     5701 cgttgtagag gcgctgaaag cgacgcaatc gctcgagctg ttcgcgcagc gtcctggccg
     5761 gcggcgccac gccggccgat cgccgtcaac ccgcca
//
LOCUS       DATGJW010000547         5175 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1243617, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000547 DATGJW010000000
VERSION     DATGJW010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 5175)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5175)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5175
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243617"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            4..909
                     /locus_tag="VKU03_16545"
     CDS             4..909
                     /locus_tag="VKU03_16545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_014331188.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing GNAT family
                     N-acetyltransferase"
                     /protein_id="HLJ72926.1"
                     /translation="MRSFNRFYTRRIGLLEEGLSNSPFSLVEARVLYELARGEAGTAA
                     DLGRALDMDKAHLSRVLARLRGRGLVSAQPSPRHAKHRLLSLTEAGRDAFAALERGTK
                     AQMRALLSPLDREANEKLSGAMSAIRSILDRPAPQPDRSFTLRAPRVGDLGWVIHRQA
                     ALYAEEYGWDWTFEGLVATIVGRFVAEFDGAKEQAWIAERHGAIVGSIFLVRGDEEGV
                     GKLRLLYVEPSARGLGIGAALTRACIERAKASGYRKLVLWTNDVLVAARRIYQAAGFT
                     LVAEERRHSFGRDLVGQNWALDLQS"
     gene            complement(919..3126)
                     /locus_tag="VKU03_16550"
     CDS             complement(919..3126)
                     /locus_tag="VKU03_16550"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016734379.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="amylo-alpha-1,6-glucosidase"
                     /protein_id="HLJ72927.1"
                     /translation="MEKSGAKLHVAGAEGLEALPAPFFIPAATPLQERRPFTLKYGDI
                     FAVFDHNGDAASAPGRPEGIFYRDTRHLSHLFLTINGARPMLLSSALRDDNATLTCDV
                     ANPDLYDPEGRLVFAHDLIHIRRSRFLWNAACFERIFVRNFDERPREIQIGLAFAADF
                     ADLFEVRGNHRDKRGETHSPAVERSFVTLSYTGLDEVRRSTVLRFDPAPTRLLRDQAI
                     FRFELGPRESRSLFLEVNCASEGPGQLTRRAFLGGLRDARRALRRSALRAAAVATSNA
                     VFNEAVRRSVSDLYMLITDLPEGPYPYAGIPWFSTVFGRDGLITALETLWLDPMIARG
                     VLGHLAANQATAIDPEADAEPGKILHEVRHGEMAALGEVPFRKYYGSIDSTPLFVMLA
                     GAYLERTGDVAFLRKVWPHVAAALDWIERFGDRDGDGFIEYGRRSGEGLVNQGWKDSS
                     DSIFHADGALARGPIAVVEAQAYVYAAWRAAEAIAQRLGDPDGAAKFAAKAERLRRHF
                     DESFFDEKLGTYILALDGDKQPCRVRSSNAGHALFAGVAYPERAAKVVRSLMGSALFC
                     GWGVRTIATTEARYNPMSYHNGSVWPHDNALIAAGFARYGFRQDAAKIFEGVFAASTF
                     VDLRRLPELFCGFPRQRTQGPTSYPVACIPQAWAAAAPLSMIQSCIGLVFNPDELRVT
                     FEEPVLPSFLDEIVLYGLRLKDASANIALRRSGRHVVVDVLDRTRAIKVLASS"
     gene            complement(3152..4240)
                     /locus_tag="VKU03_16555"
     CDS             complement(3152..4240)
                     /locus_tag="VKU03_16555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003576789.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HLJ72928.1"
                     /translation="MRIAQIAPLAESCPPRLYGGTERIVAYLTEALTRQGHDVTLFAS
                     GDSLTSARLESCCETALRLDPNVKDPIAHHLIMLDKVRRRAGEFDILHFHTEILHYPL
                     IRDFSERTVTTLHGRLDLPDLKAVYAAFADLPVVSISKDQRRPIPNANWVGTVHHGLP
                     RDLLPFNPNPGGYLAFLGRISPEKGPDRAIEIATRAGMKLKMAAKIDKSEEAYWRGAI
                     KPMVESNPNVEFVGEIAERHKADFLGNAAAVLFPIDWPEPFGLVMIEAMACGTPVIAF
                     RMGSAPEIIEDGVSGFLVDNIEEAVAAVKRVPLLDRAEGRRAFEERFTVDRMTQDYLA
                     IYRARAGLQTGAFRVRRPAINRDLHVVA"
     gene            complement(4409..>5175)
                     /gene="prpE"
                     /locus_tag="VKU03_16560"
                     /gene_synonym="yahU"
     CDS             complement(4409..>5175)
                     /gene="prpE"
                     /locus_tag="VKU03_16560"
                     /gene_synonym="yahU"
                     /EC_number="6.2.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_012114483.1"
                     /note="catalyzes the formation of propionyl-CoA using
                     propionate as a substrate; PrpE from Ralstonia
                     solanacearum can produce acetyl-, propionyl-, butyryl- and
                     acrylyl-coenzyme A, and Salmonella enterica produces
                     propionyl- and butyryl-coenzyme A; not expressed in
                     Escherichia coli when grown on propionate/minimal media;
                     ATP-dependent; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="propionyl-CoA synthetase"
                     /protein_id="HLJ72929.1"
                     /translation="ETLAWAGRLLNIPVVDHWWQTESGWPIVANPVGLGLLPIKPGSP
                     TVPMPGYDVAVVDDTAKPVKAGAMGSIVIKLPLPPGALPTLFQNDQRMVESYLSEFPG
                     YYKTSDAGYCDEDGYLFVMGRTDDIINVAGHRLSTGGMEEALASHRDVAECAVIGVKD
                     EIKGEAPVGFLVLKAGVNRAPGEIEREVVALVRERIGPVAAFKRAIAVPRLPKTRSGK
                     ILRGTMKKIADGENWAMPATIDDPAALDEIAEALKR"
BASE COUNT          967 a         1680 c         1678 g          850 t
ORIGIN      
        1 gaaatccgga gcttcaaccg cttctacacg cgccggatcg gcctgctcga agagggcctt
       61 tccaacagcc cgttctcgtt ggtcgaggcg cgggtccttt acgaattggc gcgcggcgaa
      121 gccggcacgg cggccgatct cggccgcgcg ctcgatatgg acaaggcgca tctcagccgc
      181 gtcctcgcgc gcctgcgcgg ccgcggcctc gtctcggccc agccgagccc tcgccacgcc
      241 aagcaccggc tgctctcttt gaccgaggcg gggcgtgacg cctttgcggc gctggagcgc
      301 ggaaccaagg ctcagatgcg cgccctgctc tcgccgctcg atcgcgaggc gaatgaaaaa
      361 ctgagcggcg cgatgagcgc gatccgctcg attctcgacc ggcccgcgcc gcaaccggac
      421 cggtccttca cgctgcgcgc gccgcgcgtc ggcgatctcg gctgggtcat tcaccgccag
      481 gcggcgctct atgccgagga atatggctgg gattggacgt tcgaaggctt ggtcgccacg
      541 atcgtcggac ggttcgtcgc ggaattcgac ggcgcgaaag aacaagcctg gatcgccgaa
      601 cgccacggcg cgatcgtcgg ctcgattttc ctggtccgcg gcgacgagga gggcgtcggc
      661 aagctgcgcc tcctctatgt cgagccgagc gcgcgcggcc tcggcatcgg cgcggcgttg
      721 accagagcct gcatcgagcg agccaaagcc agcggctatc gaaagctggt cctatggacc
      781 aatgacgtgc tggtcgccgc ccgccgcatc taccaagccg ccgggttcac gctcgtcgcc
      841 gaggagaggc gccattcttt cggccgcgat ctggtcgggc agaattgggc gctcgacctg
      901 caaagctgac atgcgccctc aacttgaggc cagcacctta atcgccctgg tgcggtcgag
      961 cacatcgacc accacatgac ggccggagcg ccggagcgcg atgttggccg aggcgtcttt
     1021 cagccgcaat ccgtagagca cgatttcgtc gaggaagctc ggcaagacag gctcctcgaa
     1081 cgtcacgcgc aattcgtcgg gattgaagac caggccgatg caggactgaa tcatcgacag
     1141 cggcgcggcg gcggcccagg cttgcgggat gcaggcgacg ggatacgagg tcgggccctg
     1201 cgtgcgctgg cggggaaagc cgcagaacag ctccggcaag cgcctgagat caacaaacgt
     1261 cgaggcggcg aacacgcctt cgaaaatctt cgccgcgtcc tgccggaatc cgtaccgggc
     1321 gaagccggcg gcgatcagcg cattgtcatg cggccagacc gagccgttgt gatagctcat
     1381 cggattgtag cgagcctcgg ttgtggcgat ggtgcgcacg ccccagccgc aaaaaagcgc
     1441 gcttcccatt agcgagcgaa cgaccttggc ggcccgctcg ggataggcga cgccggcgaa
     1501 gagggcgtgg ccggcattgg acgagcgcac cctgcagggc tgcttatcgc cgtcgagagc
     1561 gagaatatag gttccgagtt tctcgtcgaa aaagctctcg tcgaaatggc ggcgcaagcg
     1621 ctccgccttg gccgcgaact tcgccgcgcc gtcgggatcg ccgaggcgct gcgcgatcgc
     1681 ttcggcggcg cgccatgcgg cgtagacata ggcctgcgcc tcgaccacag cgatcggccc
     1741 gcgcgccaag gcgccgtccg cgtggaaaat ggaatcggaa ctgtccttcc agccctgatt
     1801 gaccaggccc tcgccggagc ggcggccata ttcgatgaag ccgtcgccgt cgcgatcgcc
     1861 gaagcgctcg atccaatcga gcgccgccgc gacatgcggc cagaccttgc gaagaaaagc
     1921 gacatcgccg gtccgttcaa gataagcgcc ggcgagcatg acgaagagag gcgtcgaatc
     1981 gatgcttccg taatatttgc ggaacggcac ttcgccaagc gccgccattt cgccgtgccg
     2041 aacctcgtga agaatcttgc cgggctcggc gtcagcctcg ggatcgatcg ccgtcgcctg
     2101 gttggcggcg agatggccga gcacgccgcg ggcgatcatc ggatcgagcc agagcgtttc
     2161 gagcgcagtg atcagcccgt cgcgaccgaa gacggtgctg aaccacggta tgcccgcata
     2221 gggataaggc ccctccggca ggtcggtgat cagcatgtag agatcggaga cgctgcgccg
     2281 caccgcttcg ttgaaaacgg cgttcgacgt cgcgaccgcc gccgcgcgca gcgccgagcg
     2341 ccgcagggcg cggcgcgcgt cgcgcaaccc gccgagaaac gcccgccgcg tcaattggcc
     2401 ggggccttcc gaggcgcaat tgacctcgag gaacagggag cgcgattcgc gcggccccaa
     2461 ttcgaatctg aagatggcct ggtcgcgcag caggcgcgtc ggcgccggat cgaagcggag
     2521 aaccgtcgag cgacgtactt cgtccaatcc cgtatagctc agcgtgacga agctccgctc
     2581 gaccgccggg ctatgcgttt cgcccctttt gtcgcgatga ttgccgcgaa cctcgaaaag
     2641 atcggcgaaa tcggccgcga aggcgaggcc gatctgaatc tctctcggcc gctcatcgaa
     2701 atttctgacg aagatccgct cgaagcaggc ggcgttccag agaaagcgcg aacggcggat
     2761 atggatgaga tcgtgcgcga agacgagccg gccttcggga tcatagagat cgggattggc
     2821 gacgtcgcag gtcagcgtcg cattgtcgtc gcgcaaagcc gagctcagca gcatcggccg
     2881 cgcgccgtta atggtcaaaa acagatggga gaggtggcgc gtgtcgcgat agaatattcc
     2941 ctctggccgg ccgggcgcgg aggcggcgtc gccattatgg tcgaacacgg cgaagatatc
     3001 gccgtattta agcgtgaacg gccgccgctc ctgcagcggc gtggcggccg gaatgaaaaa
     3061 cggcgccggc aaagcttcaa gaccctccgc gccggccacg tgcaacttgg cgccgctctt
     3121 ttccattcag cccccaaacg aaagccgccg cttaggcgac gacgtgaaga tcgcgattga
     3181 tcgccgggcg gcgaacacgg aaggcgccgg tctgcaagcc ggcgcgcgcg cggtagatcg
     3241 cgagatagtc ctgcgtcatg cggtcgacag tgaagcgctc ttcgaaggcg cgccgtcctt
     3301 cggctcgatc gagcaaaggg acgcgcttca ccgccgcgac cgcctcctcg atattgtcga
     3361 caagaaatcc cgacacgccg tcctcgatga tttccggcgc cgagcccatc cgaaacgcga
     3421 tgaccggcgt gccgcaagcc atcgcctcga tcatgaccag cccgaacggt tcgggccaat
     3481 cgatcggaaa aagcaccgcc gccgcattgc ccaaaaaatc cgccttgtgt cgctcggcga
     3541 tctcgccgac aaattccaca ttgggattgc tttcgaccat cggcttgatc gcgcctcgcc
     3601 agtaggcctc ttcgctcttg tcgattttcg ccgccatttt cagcttcatg ccggcccgtg
     3661 tggcgatttc aatggcccga tcggggcctt tctcgggcga aatccgcccc aaaaacgcaa
     3721 gatagccgcc gggattggga ttgaagggca aaagatcgcg cggcaggccg tgatgcaccg
     3781 tcccgaccca attggcgttg ggaatcggcc ggcgctgatc cttggagatc gagacgaccg
     3841 gcaggtcggc gaaggccgcg tacaccgctt tgaggtccgg cagatcgagg cgcccatgca
     3901 gcgtcgtcac cgtccgctcg gaaaaatcgc ggatcagcgg ataatgcaaa atctcggtat
     3961 ggaagtggag aatgtcgaac tcgccggccc ggcgccggac cttgtcgagc atgatcagat
     4021 gatgggcgat cgggtccttg acattgggat cgaggcgcag cgccgtctcg cagcacgatt
     4081 cgaggcgggc tgaggtcagc gaatcgccgc tggcgaaaag cgttacgtca tggccctgac
     4141 gcgtcagcgc ctcggtcagg taagcaacga tacgttccgt gccgccatag agccggggcg
     4201 ggcagctttc ggcaagcggg gcaatttgag caatcctcat ccatcatctc ctcgcatgcg
     4261 atgccggtgc tgaacggcaa cgccgcgcgg agcatcacgc gccgagaaac gcgcgaggcc
     4321 gcccgggagt tcatcataag cgcattagcg aggcgaaagc tatgcgccgc gcagcccatc
     4381 tgaaaccgcg aaattttaca gacgtccgtc agcgcttgag cgcctcggcg atctcgtcca
     4441 gggcggccgg atcgtcgatc gtcgccggca tcgcccaatt ttcgccatcg gcgatcttct
     4501 tcattgtgcc gcgcagaatc ttgccggatc gcgtcttcgg cagacgcggg acggcgatcg
     4561 cccgcttgaa cgccgcgacc ggcccgatgc gctcgcgcac cagcgcgacg acttcgcgct
     4621 cgatctcgcc cggcgcgcgg ttgacgcccg ccttgagcac cagaaagccg accggcgcct
     4681 cgcccttgat ctcgtccttg acgccgatca ccgcgcattc ggcgacgtct cgatgcgacg
     4741 cgagagcttc ctccatgccg ccggtcgaca gccgatggcc ggcgacattg atgatgtcgt
     4801 cggtgcggcc catcacgaag agataaccgt cctcgtcgca atagccggcg tccgacgtct
     4861 tgtagtagcc gggaaattcg gacaaatagc tctcgaccat ccgctgatcg ttttggaaca
     4921 aagtcggcag cgcgccgggc ggcagcggca gcttgatcac gatcgagccc atcgcgccgg
     4981 ctttgaccgg tttcgctgta tcgtcgacca ccgcgacgtc gtaccccggc atgggaaccg
     5041 tcggcgagcc tggcttgatc ggcaacaggc cgaggccgac cggattggcg acgatcggcc
     5101 agccgctctc ggtctgccac cagtgatcga cgaccgggat attgagcaat cggccggccc
     5161 aggcgagcgt ttcag
//
LOCUS       DATGJW010000548         2516 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Roseiarcus sp. isolate SMAG_U9843 k141_1243882, whole
            genome shotgun sequence.
ACCESSION   DATGJW010000548 DATGJW010000000
VERSION     DATGJW010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37965411
            Sequence Read Archive: SRR5579872
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Roseiarcus sp. (soil metagenome)
  ORGANISM  Roseiarcus sp.
            Bacteria; Pseudomonadota; Alphaproteobacteria; Hyphomicrobiales;
            Roseiarcaceae; Roseiarcus.
REFERENCE   1  (bases 1 to 2516)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2516)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/03/2024 12:25:22
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 3,315
            CDSs (total)                      :: 3,288
            Genes (coding)                    :: 3,265
            CDSs (with protein)               :: 3,265
            Genes (RNA)                       :: 27
            rRNAs                             :: 1 (16S)
            partial rRNAs                     :: 1 (16S)
            tRNAs                             :: 22
            ncRNAs                            :: 4
            Pseudo Genes (total)              :: 23
            CDSs (without protein)            :: 23
            Pseudo Genes (ambiguous residues) :: 0 of 23
            Pseudo Genes (frameshifted)       :: 8 of 23
            Pseudo Genes (incomplete)         :: 10 of 23
            Pseudo Genes (internal stop)      :: 5 of 23
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2516
                     /organism="Roseiarcus sp."
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1243882"
                     /isolate="SMAG_U9843"
                     /isolation_source="soil"
                     /db_xref="taxon:1969460"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="40.7999 N 76.3402 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..798
                     /locus_tag="VKU03_16565"
     CDS             <1..798
                     /locus_tag="VKU03_16565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008961531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ72930.1"
                     /translation="CQGYSEPGAGSDLASLRTKCEDRGDHFLVNGQKIWTSYADKADW
                     IFCLVRTDPAAKKQQGISFVLIDMATKGVSTKPIVLISGKSPFCETFFDNVQVPKENL
                     VGTLNRGWDIAKYLLTHERGAISGGGLNVFDNEALNKAASAAAKASGGRLADPILRAD
                     IARAAIDTYASAATVERYMQEAAAGGELGARSATLKYDGAELNKRRNELMMSLAGHEG
                     LYFDGNLGPASDIAQRWLRSKGNSIEGGTSEIMLDIIAKRILELPGE"
     gene            818..1927
                     /locus_tag="VKU03_16570"
     CDS             818..1927
                     /locus_tag="VKU03_16570"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011661987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HLJ72931.1"
                     /translation="MLVLSEEQRLLRDSAEAALAAKAPVSLWRKLRAENDEAGYSRDF
                     WRECAAAGWAGILVPEAYDGLDFGLAGAGLVAREMGRTLAASPFLSTAVMAASALKSG
                     GSESQKKLWLPKIAAGEAVVAVAIDEEARHNPAHIATIATKTEKGWRLDGAKACVLDG
                     AIADVLLIAAKSGDDVGLYLVDAHASGLSRVARTLVDGRRVATVRLDNVEAPADAKLG
                     GGAALIDNILDAARGVLAASLCGVAEEAFARTLDYMKQRKQFDRTIGSFQSLQHRVAT
                     THVAIENAWSASFKALQALDAGAADSGLQIAMAKAKAGDTGRTTTAECLQLHGGIGMT
                     DEHDIGLFMKKARVDAELFGDPAYHADRVATMLGY"
     gene            1939..>2516
                     /locus_tag="VKU03_16575"
     CDS             1939..>2516
                     /locus_tag="VKU03_16575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_009210934.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HLJ72932.1"
                     /translation="MFKLEDLFSVKGRTALVTGGATGIGRVCAEALLAAGARVLIASR
                     KADACEAVAKELSAYGPCEGFGGTVASDDGVKALSAELHRRTDKLDILVNNAGTTWGA
                     PFETFEWKAWERVLSVNVAGLFQLTRDAAPILFKAATADRPANVINIGSVMGTVTQSG
                     SAYSYSASKAAVHHLTRILAAEFAGRFVTVNAL"
BASE COUNT          465 a          799 c          819 g          433 t
ORIGIN      
        1 tgtcaaggct attcggagcc gggcgccggc tccgacctcg cctccttgcg caccaaatgc
       61 gaggaccgcg gcgatcattt cctcgtcaat ggtcagaaga tatggacgtc ctacgccgac
      121 aaggccgatt ggattttctg cctcgttcgt accgatcccg cggccaagaa gcagcagggc
      181 atttccttcg tcctgatcga catggcgacg aagggggtca gcaccaagcc gattgtcttg
      241 atctccggca agtcgccgtt ctgcgagacg ttcttcgaca atgttcaggt tcccaaggaa
      301 aatctcgtcg ggacgctcaa tcgcggctgg gacatcgcca aatatctttt gacccatgag
      361 cgcggcgcga tcagcggcgg tggcctcaat gttttcgaca atgaggcgtt gaacaaggcc
      421 gccagcgccg cagccaaggc ttccggcggc cgtctcgccg atccgatttt gcgcgccgac
      481 atcgccagag ccgcgatcga cacttacgcc tccgccgcga ccgtggaaag gtacatgcag
      541 gaggcggcgg ccggcggcga actcggcgcg cgttcggcga cgctcaaata tgacggcgcc
      601 gagctcaaca agcgccgcaa cgaattgatg atgtccctcg ccgggcacga aggcctctac
      661 ttcgacggca atctgggtcc ggccagcgat atcgcgcagc gctggctgcg cagcaaaggc
      721 aattcgatcg aaggcggcac gtcggaaatc atgctcgaca tcatcgccaa gcgtattttg
      781 gagttgccgg gggaatgagc cagtcgaagg aatcagcatg ctcgtcctaa gcgaagaaca
      841 gcgcctgctg cgggactcgg ctgaggccgc gctcgccgcc aaggcgcccg tctcgctgtg
      901 gcgcaagctg cgcgccgaga atgacgaggc gggctattcg cgcgatttct ggcgcgaatg
      961 cgcggcggcg ggctgggcgg gaatcctggt tccagaagcc tatgacggtc tcgatttcgg
     1021 gctcgccggc gcagggctcg tcgcccgcga gatggggcgg accctcgcgg cttcgccctt
     1081 cctctcgacg gcggtgatgg cggcgagcgc gctgaagagc ggaggttcgg agagccagaa
     1141 gaagctttgg ctgccgaaga tcgccgccgg cgaagcggtc gtcgcggtcg ccattgacga
     1201 agaggcgcgg cataatcccg cgcatatcgc gaccatcgca acgaagaccg agaagggctg
     1261 gcgtttggat ggcgccaagg cctgcgtgct cgacggcgcg atcgccgatg tgctccttat
     1321 cgcggcgaag tcgggcgacg acgtcggatt gtacctggtc gacgcgcacg catccggcct
     1381 ttcgcgcgtg gcccgcacgc tggtcgacgg ccgacgcgtc gcgaccgtca ggctcgacaa
     1441 tgtcgaagcg ccggccgatg caaaactcgg tggcggcgct gctttgatcg ataatatcct
     1501 cgacgcggcg cgcggcgttc tcgccgcctc gctttgcggc gtcgccgagg aggcattcgc
     1561 ccgcacgctc gactatatga aacagcgcaa gcagttcgat cggacaatcg gcagcttcca
     1621 gtcgctgcag catcgcgtcg cgacgacgca tgtcgcgatc gagaacgcct ggtcggcgag
     1681 tttcaaggcg ctgcaggcgc tcgatgcggg cgccgccgac tcagggctcc agatcgctat
     1741 ggccaaagcc aaggccggcg acactgggcg cacgacaacc gccgaatgtc tgcaattgca
     1801 cggcggcatc ggcatgaccg acgaacacga catcggcctc ttcatgaaaa aggcccgtgt
     1861 cgacgccgaa ttgttcggcg atcccgcgta tcacgccgat cgcgtcgcga ccatgctcgg
     1921 ctactgaagg ggaaagagtt gttcaagctc gaggacttgt tctcggtcaa aggccgcacc
     1981 gcactggtca ccggcggtgc gacgggcatc ggtcgcgtct gcgccgaagc tttgcttgcc
     2041 gccggcgcgc gagtgctgat cgcgtcgcgc aaagccgacg cctgcgaggc cgtggcgaag
     2101 gaactctcgg cgtacggccc ttgcgagggc tttggcggga cagtcgccag cgacgacggc
     2161 gtcaaagcgc tcagcgccga actccaccgc cgtaccgaca agctggacat tctcgtcaac
     2221 aacgccggta caacctgggg cgcgccgttc gagaccttcg aatggaaagc ctgggagcgc
     2281 gtgctgtccg tcaatgtcgc cgggctcttt caattgaccc gcgacgccgc gccgattctc
     2341 ttcaaggccg cgacggccga caggccggcg aatgtcatca atatcggctc cgtcatgggc
     2401 acggtcacgc aaagcggcag cgcctactcc tattcggcat cgaaagcggc ggtgcatcat
     2461 ctgacgcgca ttctggcggc ggaattcgcc ggccgcttcg ttacggtcaa tgcgct
//
